LOCUS       DAEVZA010000001        99776 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEVZA010000001 DAEVZA010000000
VERSION     DAEVZA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 99776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.001"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..89)
                     /locus_tag="LAF92_000001"
     CDS             complement(<1..89)
                     /locus_tag="LAF92_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically inducible protein OsmC"
                     /protein_id="HBI9789074.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSG"
     gene            391..1023
                     /gene="crp"
                     /locus_tag="LAF92_000002"
     CDS             391..1023
                     /gene="crp"
                     /locus_tag="LAF92_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBI9789075.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEDQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            1074..3164
                     /locus_tag="LAF92_000003"
     CDS             1074..3164
                     /locus_tag="LAF92_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI9789076.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(3231..4451)
                     /gene="argD/dapC"
                     /locus_tag="LAF92_000004"
     CDS             complement(3231..4451)
                     /gene="argD/dapC"
                     /locus_tag="LAF92_000004"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBI9789077.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(4537..5100)
                     /gene="pabA"
                     /locus_tag="LAF92_000005"
     CDS             complement(4537..5100)
                     /gene="pabA"
                     /locus_tag="LAF92_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBI9789078.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(5132..5734)
                     /locus_tag="LAF92_000006"
     CDS             complement(5132..5734)
                     /locus_tag="LAF92_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBI9789079.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(5724..5891)
                     /locus_tag="LAF92_000007"
     CDS             complement(5724..5891)
                     /locus_tag="LAF92_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBI9789080.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(5996..6568)
                     /gene="ppiA"
                     /locus_tag="LAF92_000008"
                     /gene_synonym="rotA"
     CDS             complement(5996..6568)
                     /gene="ppiA"
                     /locus_tag="LAF92_000008"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBI9789081.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            6839..8020
                     /gene="tsgA"
                     /locus_tag="LAF92_000009"
     CDS             6839..8020
                     /gene="tsgA"
                     /locus_tag="LAF92_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBI9789082.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            8282..10825
                     /gene="nirB"
                     /locus_tag="LAF92_000010"
     CDS             8282..10825
                     /gene="nirB"
                     /locus_tag="LAF92_000010"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBI9789083.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            10822..11148
                     /gene="nirD"
                     /locus_tag="LAF92_000011"
     CDS             10822..11148
                     /gene="nirD"
                     /locus_tag="LAF92_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBI9789084.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            11276..12082
                     /gene="nirC"
                     /locus_tag="LAF92_000012"
     CDS             11276..12082
                     /gene="nirC"
                     /locus_tag="LAF92_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBI9789085.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            12101..13474
                     /gene="cobA"
                     /locus_tag="LAF92_000013"
     CDS             12101..13474
                     /gene="cobA"
                     /locus_tag="LAF92_000013"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBI9789086.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            13729..13896
                     /locus_tag="LAF92_000014"
     CDS             13729..13896
                     /locus_tag="LAF92_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBI9789087.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            14140..15528
                     /gene="frlA"
                     /locus_tag="LAF92_000015"
     CDS             14140..15528
                     /gene="frlA"
                     /locus_tag="LAF92_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HBI9789088.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            15549..16571
                     /gene="frlB"
                     /locus_tag="LAF92_000016"
     CDS             15549..16571
                     /gene="frlB"
                     /locus_tag="LAF92_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBI9789089.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            16621..17451
                     /gene="frlC"
                     /locus_tag="LAF92_000017"
     CDS             16621..17451
                     /gene="frlC"
                     /locus_tag="LAF92_000017"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBI9789090.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            17448..18233
                     /gene="frlD"
                     /locus_tag="LAF92_000018"
     CDS             17448..18233
                     /gene="frlD"
                     /locus_tag="LAF92_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBI9789091.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            18333..19064
                     /gene="phoB"
                     /locus_tag="LAF92_000019"
     CDS             18333..19064
                     /gene="phoB"
                     /locus_tag="LAF92_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBI9789092.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(19216..20301)
                     /locus_tag="LAF92_000020"
     CDS             complement(19216..20301)
                     /locus_tag="LAF92_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI9789093.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(20313..21617)
                     /locus_tag="LAF92_000021"
     CDS             complement(20313..21617)
                     /locus_tag="LAF92_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBI9789094.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(21629..21982)
                     /locus_tag="LAF92_000022"
     CDS             complement(21629..21982)
                     /locus_tag="LAF92_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBI9789095.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(21993..22871)
                     /locus_tag="LAF92_000023"
     CDS             complement(21993..22871)
                     /locus_tag="LAF92_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBI9789096.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(22868..24094)
                     /locus_tag="LAF92_000024"
     CDS             complement(22868..24094)
                     /locus_tag="LAF92_000024"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBI9789097.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(24094..25257)
                     /locus_tag="LAF92_000025"
     CDS             complement(24094..25257)
                     /locus_tag="LAF92_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBI9789098.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(25341..25703)
                     /locus_tag="LAF92_000026"
     CDS             complement(25341..25703)
                     /locus_tag="LAF92_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBI9789099.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(25720..26625)
                     /locus_tag="LAF92_000027"
     CDS             complement(25720..26625)
                     /locus_tag="LAF92_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789100.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGCNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(26894..27898)
                     /gene="trpS"
                     /locus_tag="LAF92_000028"
     CDS             complement(26894..27898)
                     /gene="trpS"
                     /locus_tag="LAF92_000028"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBI9789101.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(27891..28649)
                     /gene="gph"
                     /locus_tag="LAF92_000029"
     CDS             complement(27891..28649)
                     /gene="gph"
                     /locus_tag="LAF92_000029"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBI9789102.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQEMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(28642..29319)
                     /gene="rpe"
                     /locus_tag="LAF92_000030"
     CDS             complement(28642..29319)
                     /gene="rpe"
                     /locus_tag="LAF92_000030"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBI9789103.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(29337..30173)
                     /gene="dam"
                     /locus_tag="LAF92_000031"
     CDS             complement(29337..30173)
                     /gene="dam"
                     /locus_tag="LAF92_000031"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBI9789104.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(30280..31566)
                     /gene="damX"
                     /locus_tag="LAF92_000032"
     CDS             complement(30280..31566)
                     /gene="damX"
                     /locus_tag="LAF92_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBI9789105.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(31658..32746)
                     /gene="aroB"
                     /locus_tag="LAF92_000033"
     CDS             complement(31658..32746)
                     /gene="aroB"
                     /locus_tag="LAF92_000033"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBI9789106.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(32803..33324)
                     /gene="aroK"
                     /locus_tag="LAF92_000034"
     CDS             complement(32803..33324)
                     /gene="aroK"
                     /locus_tag="LAF92_000034"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBI9789107.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(33725..34963)
                     /gene="hofQ"
                     /locus_tag="LAF92_000035"
     CDS             complement(33725..34963)
                     /gene="hofQ"
                     /locus_tag="LAF92_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBI9789108.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(34875..35279)
                     /gene="hofP"
                     /locus_tag="LAF92_000036"
     CDS             complement(34875..35279)
                     /gene="hofP"
                     /locus_tag="LAF92_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBI9789109.1"
                     /translation="MRVKRWLLAGIGLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(35269..35709)
                     /gene="hofO"
                     /locus_tag="LAF92_000037"
     CDS             complement(35269..35709)
                     /gene="hofO"
                     /locus_tag="LAF92_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBI9789110.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(35693..36232)
                     /gene="hofN"
                     /locus_tag="LAF92_000038"
     CDS             complement(35693..36232)
                     /gene="hofN"
                     /locus_tag="LAF92_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBI9789111.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(36232..37011)
                     /gene="hofM"
                     /locus_tag="LAF92_000039"
     CDS             complement(36232..37011)
                     /gene="hofM"
                     /locus_tag="LAF92_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBI9789112.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            37131..39683
                     /gene="mrcA"
                     /locus_tag="LAF92_000040"
     CDS             37131..39683
                     /gene="mrcA"
                     /locus_tag="LAF92_000040"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBI9789113.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(39848..40408)
                     /gene="nudE"
                     /locus_tag="LAF92_000041"
     CDS             complement(39848..40408)
                     /gene="nudE"
                     /locus_tag="LAF92_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBI9789114.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            40728..42863
                     /gene="igaA"
                     /locus_tag="LAF92_000042"
     CDS             40728..42863
                     /gene="igaA"
                     /locus_tag="LAF92_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBI9789115.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            42928..43596
                     /gene="yrfG"
                     /locus_tag="LAF92_000043"
     CDS             42928..43596
                     /gene="yrfG"
                     /locus_tag="LAF92_000043"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBI9789116.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLKHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYERHPSLNDYRRLI
                     PSLM"
     gene            43607..44008
                     /gene="hslR"
                     /locus_tag="LAF92_000044"
     CDS             43607..44008
                     /gene="hslR"
                     /locus_tag="LAF92_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBI9789117.1"
                     /translation="MKEKPVAEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            44033..44911
                     /gene="hslO"
                     /locus_tag="LAF92_000045"
     CDS             44033..44911
                     /gene="hslO"
                     /locus_tag="LAF92_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBI9789118.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMSLAVINGNNKQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(44974..46698)
                     /locus_tag="LAF92_000046"
                     /pseudo
     CDS             complement(44974..46698)
                     /locus_tag="LAF92_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            47076..48698
                     /gene="pckA"
                     /locus_tag="LAF92_000047"
     CDS             47076..48698
                     /gene="pckA"
                     /locus_tag="LAF92_000047"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBI9789119.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            48815..49132
                     /locus_tag="LAF92_000048"
     CDS             48815..49132
                     /locus_tag="LAF92_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBI9789120.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            49191..49487
                     /locus_tag="LAF92_000049"
     CDS             49191..49487
                     /locus_tag="LAF92_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI9789121.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(49519..50871)
                     /gene="envZ"
                     /locus_tag="LAF92_000050"
     CDS             complement(49519..50871)
                     /gene="envZ"
                     /locus_tag="LAF92_000050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBI9789122.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(50868..51587)
                     /gene="ompR"
                     /locus_tag="LAF92_000051"
     CDS             complement(50868..51587)
                     /gene="ompR"
                     /locus_tag="LAF92_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBI9789123.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            51815..52291
                     /gene="greB"
                     /locus_tag="LAF92_000052"
     CDS             51815..52291
                     /gene="greB"
                     /locus_tag="LAF92_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBI9789124.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            52388..54709
                     /locus_tag="LAF92_000053"
     CDS             52388..54709
                     /locus_tag="LAF92_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBI9789125.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            55147..55374
                     /gene="feoA"
                     /locus_tag="LAF92_000054"
     CDS             55147..55374
                     /gene="feoA"
                     /locus_tag="LAF92_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBI9789126.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            55391..57712
                     /gene="feoB"
                     /locus_tag="LAF92_000055"
     CDS             55391..57712
                     /gene="feoB"
                     /locus_tag="LAF92_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBI9789127.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            57712..57948
                     /gene="feoC"
                     /locus_tag="LAF92_000056"
     CDS             57712..57948
                     /gene="feoC"
                     /locus_tag="LAF92_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBI9789128.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            58151..59029
                     /gene="rpnA"
                     /locus_tag="LAF92_000057"
                     /pseudo
     CDS             58151..59029
                     /gene="rpnA"
                     /locus_tag="LAF92_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
     gene            complement(59058..59828)
                     /gene="bioH"
                     /locus_tag="LAF92_000058"
     CDS             complement(59058..59828)
                     /gene="bioH"
                     /locus_tag="LAF92_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBI9789129.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            59866..60549
                     /gene="gntX"
                     /locus_tag="LAF92_000059"
     CDS             59866..60549
                     /gene="gntX"
                     /locus_tag="LAF92_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBI9789130.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            60608..61183
                     /gene="nfuA"
                     /locus_tag="LAF92_000060"
     CDS             60608..61183
                     /gene="nfuA"
                     /locus_tag="LAF92_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBI9789131.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            61543..62859
                     /gene="gntT"
                     /locus_tag="LAF92_000061"
     CDS             61543..62859
                     /gene="gntT"
                     /locus_tag="LAF92_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBI9789132.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(62904..64988)
                     /gene="malQ"
                     /locus_tag="LAF92_000062"
     CDS             complement(62904..64988)
                     /gene="malQ"
                     /locus_tag="LAF92_000062"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBI9789133.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAIRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(64998..67391)
                     /gene="malP"
                     /locus_tag="LAF92_000063"
     CDS             complement(64998..67391)
                     /gene="malP"
                     /locus_tag="LAF92_000063"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBI9789134.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            68003..70708
                     /gene="malT"
                     /locus_tag="LAF92_000064"
     CDS             68003..70708
                     /gene="malT"
                     /locus_tag="LAF92_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBI9789135.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFNHQEAKQFFDRRLTSPIEAAESSRMCDDVSGW
                     ATALQLIALSARQNSHSAHQSARRLSGINASHLSDYLVDEVLNNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(70751..71767)
                     /gene="rtcA"
                     /locus_tag="LAF92_000065"
     CDS             complement(70751..71767)
                     /gene="rtcA"
                     /locus_tag="LAF92_000065"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBI9789136.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(71771..72997)
                     /gene="rtcB"
                     /locus_tag="LAF92_000066"
     CDS             complement(71771..72997)
                     /gene="rtcB"
                     /locus_tag="LAF92_000066"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBI9789137.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDEADQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            73186..74784
                     /gene="rtcR"
                     /locus_tag="LAF92_000067"
     CDS             73186..74784
                     /gene="rtcR"
                     /locus_tag="LAF92_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBI9789138.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(74766..75524)
                     /locus_tag="LAF92_000068"
     CDS             complement(74766..75524)
                     /locus_tag="LAF92_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBI9789139.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(75541..76371)
                     /gene="glpG"
                     /locus_tag="LAF92_000069"
     CDS             complement(75541..76371)
                     /gene="glpG"
                     /locus_tag="LAF92_000069"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBI9789140.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(76416..76742)
                     /gene="glpE"
                     /locus_tag="LAF92_000070"
     CDS             complement(76416..76742)
                     /gene="glpE"
                     /locus_tag="LAF92_000070"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBI9789141.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            76932..78437
                     /gene="glpD"
                     /locus_tag="LAF92_000071"
     CDS             76932..78437
                     /gene="glpD"
                     /locus_tag="LAF92_000071"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBI9789142.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(78491..79096)
                     /locus_tag="LAF92_000072"
     CDS             complement(78491..79096)
                     /locus_tag="LAF92_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789143.1"
                     /translation="MIKFQLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(79099..79866)
                     /locus_tag="LAF92_000073"
     CDS             complement(79099..79866)
                     /locus_tag="LAF92_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789144.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(79869..80579)
                     /locus_tag="LAF92_000074"
     CDS             complement(79869..80579)
                     /locus_tag="LAF92_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789145.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(80594..82102)
                     /locus_tag="LAF92_000075"
     CDS             complement(80594..82102)
                     /locus_tag="LAF92_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBI9789146.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(82227..84674)
                     /gene="glgP"
                     /locus_tag="LAF92_000076"
     CDS             complement(82227..84674)
                     /gene="glgP"
                     /locus_tag="LAF92_000076"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBI9789147.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDSQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(84693..86126)
                     /gene="glgA"
                     /locus_tag="LAF92_000077"
     CDS             complement(84693..86126)
                     /gene="glgA"
                     /locus_tag="LAF92_000077"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBI9789148.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(86126..87421)
                     /gene="glgC"
                     /locus_tag="LAF92_000078"
     CDS             complement(86126..87421)
                     /gene="glgC"
                     /locus_tag="LAF92_000078"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBI9789149.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(87439..89412)
                     /gene="glgX"
                     /locus_tag="LAF92_000079"
     CDS             complement(87439..89412)
                     /gene="glgX"
                     /locus_tag="LAF92_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBI9789150.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(89409..91595)
                     /gene="glgB"
                     /locus_tag="LAF92_000080"
     CDS             complement(89409..91595)
                     /gene="glgB"
                     /locus_tag="LAF92_000080"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBI9789151.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(91868..92971)
                     /gene="asd"
                     /locus_tag="LAF92_000081"
     CDS             complement(91868..92971)
                     /gene="asd"
                     /locus_tag="LAF92_000081"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBI9789152.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            93163..93756
                     /gene="yhgN"
                     /locus_tag="LAF92_000082"
     CDS             93163..93756
                     /gene="yhgN"
                     /locus_tag="LAF92_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBI9789153.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(93813..95153)
                     /gene="gntU"
                     /locus_tag="LAF92_000083"
     CDS             complement(93813..95153)
                     /gene="gntU"
                     /locus_tag="LAF92_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBI9789154.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(95157..95684)
                     /gene="gntK"
                     /locus_tag="LAF92_000084"
     CDS             complement(95157..95684)
                     /gene="gntK"
                     /locus_tag="LAF92_000084"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBI9789155.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(95823..96818)
                     /gene="gntR"
                     /locus_tag="LAF92_000085"
     CDS             complement(95823..96818)
                     /gene="gntR"
                     /locus_tag="LAF92_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBI9789156.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(97042..97737)
                     /gene="yhhW"
                     /locus_tag="LAF92_000086"
     CDS             complement(97042..97737)
                     /gene="yhhW"
                     /locus_tag="LAF92_000086"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBI9789157.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(97860..98897)
                     /locus_tag="LAF92_000087"
     CDS             complement(97860..98897)
                     /locus_tag="LAF92_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI9789158.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDELYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            99231..99719
                     /gene="yhhY"
                     /locus_tag="LAF92_000088"
     CDS             99231..99719
                     /gene="yhhY"
                     /locus_tag="LAF92_000088"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI9789159.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        24061 a        27075 c        25147 g        23493 t
ORIGIN      
        1 cctgagttgc cgtccattaa aatctgatga ccagaggcgg attcgcccag aaaagttaac
       61 ccttcgaccc acttcactcg cgcttgcatt tttgctactc cactgcgtca attttcctga
      121 cagagtacgc gtactaacca aatcgcgcaa cggaaggcga cctgggtcat gctgaagcga
      181 gacaccagga gacacaaagc gaaagctatg ctaaaacagt caggatgcta cagtaataca
      241 ttgatgtact gcatgtatgc aaaggacgtc acattaccgt gcagtacagt tgatagcccc
      301 ttcccaggta gcgggaagca tatttcggca atccagagac agcggcgtta tctggctctg
      361 gagaaagctt ataacagagg ataaccgcgc atggtgcttg gcaaaccgca aacagacccg
      421 actctcgaat ggttcttgtc tcattgccac attcataagt acccatccaa gagcacgctt
      481 attcaccagg gtgaaaaagc ggaaacgctg tactacatcg ttaaaggctc tgtggcagtg
      541 ctgatcaaag acgaagaggg taaagaaatg atcctctcct atctgaatca gggtgatttt
      601 attggcgaac tgggcctgtt tgaagaggac caggaacgta gcgcatgggt acgtgcgaaa
      661 accgcctgtg aagtggctga aatttcgtac aaaaaatttc gccaattgat tcaggtaaac
      721 ccggacattc tgatgcgtct gtctgcacag atggcgcgtc gtctgcaagt cacttcagag
      781 aaagtgggca acctggcgtt cctcgacgtg acgggccgca ttgcacagac tctgctgaac
      841 ctggcaaaac aaccagatgc tatgactcac ccggacggta tgcaaatcaa aattacccgt
      901 caggaaatcg gtcagattgt cggctgttct cgtgaaaccg tgggacgcat tctgaagatg
      961 ctggaagatc agaacctgat ctccgcacac ggtaaaacca tcgtcgttta cggcactcgt
     1021 taatcccgtc ggagtggcgc gttacctggt agcgcgccat tttgtttccc ccgatgtggc
     1081 gcagactgat ttatcacccc gatatcaact atgcacttcg acaaacgctg gtgctatgtt
     1141 tgcccgtggc cgttgggtta atgcttggcg aattacgatt cggtctgctc ttctctctcg
     1201 ttcctgcctg ttgcaatatt gcgggccttg atacgcctca taaacgtttt ttcaaacgct
     1261 taatcattgg tgcgtcgctg tttgccacct gtagcttact gacacagctg ctactggcaa
     1321 aagatgttcc cctgcccttt ttgctgaccg gattaacgct ggtacttggc gtcactgctg
     1381 agttggggcc attgcacgca aaattgcttc ccgcatcgct gctggcagcc atttttaccc
     1441 tcagtctggc gggatacatg ccggtctggg aaccattgct catctatgcg ttgggcactc
     1501 tctggtacgg attgtttaac tggttttggt tctggatctg gcgcgaacaa ccgctgcgcg
     1561 agtcactaag tctgctgtac cgtgaactgg cagattattg tgaagccaaa tacagcctgc
     1621 ttacccagca caccgatcct gaaaaagcgc tgccaccgct gctggtgcgc cagcaaaaag
     1681 cggtcgattt aattactcag tgctatcagc aaatgcatat gctttccgcg caaaacaata
     1741 ccgattacaa gcggatgctg cgtattttcc aggaggcgct ggatttgcag gaacatattt
     1801 cggtcagttt gcatcagccg gaagaggtgc aaaaactggt cgagcgtagc catgcggaag
     1861 aagttatccg ctggaatgcg caaaccgtcg ccgctcgcct gcgcgtgctg gctgatgaca
     1921 ttctttacca tcgcctgcca acgcgtttta cgatggaaaa gcaaattggc gcactggaaa
     1981 aaatcgcccg ccagcatccg gataatccgg ttgggcaatt ctgctactgg catttcagcc
     2041 gcatcgcccg cgtgctgcgc acccaaaaac cgctctatgc ccgtgactta ctggccgata
     2101 aacagcggcg aatgccatta cttccggcgc tgaaaagtta tctgtcacta aagtctccgg
     2161 cgctacgcaa tgccggacga ctcagtgtga tgttaagcgt tgccagcctg atgggcaccg
     2221 cgctgcatct gccgaagtcg tactggatcc tgatgacggt attgctggtg acacaaaatg
     2281 gctatggcgc aacccgtctg aggattgtga atcgctccgt gggaaccgtg gtcgggttaa
     2341 tcattgcggg cgtggcgctg cactttaaaa ttcccgaagg ttacaccctg acgttgatgc
     2401 tgattaccac cctcgccagc tacctgatat tgcgcaaaaa ctacggctgg gcgacggtcg
     2461 gttttactat taccgcagtg tataccctgc aactattgtg gttgaacggc gagcaataca
     2521 tccttccgcg tcttatcgat accattattg gttgtttaat tgctttcggc ggtactgtct
     2581 ggctgtggcc gcagtggcag agcgggttat tgcgtaaaaa cgcccatgac gctttagaag
     2641 cctatcagga agcgattcgc ttgattctta gcgaggatcc gcaacctacg ccactggcct
     2701 ggcagcgaat gcgggtaaat caggcacata acactctgta taactcattg aatcaggcga
     2761 tgcaggaacc ggcgtttaac agccattatc tggcagatat gaaactgtgg gtaacgcaca
     2821 gccagtttat tgttgagcat attaatgcca tgaccacgct ggcgcgggaa caccgggcat
     2881 tgccacctga actggcacaa gagtatttac agtcttgtga aatcgccatt cagcgttgtc
     2941 agcagcgact ggagtatgac gaaccgggta gttctggcga tgccaatatc atggatgcgc
     3001 cggagatgca gccgcacgaa ggcgcggcag gtacgctgga gcagcattta cagcgggtta
     3061 ttggtcatct gaacaccatg cacaccattt cgtcgatggc atggcgtcag cgaccgcatc
     3121 acgggatttg gctgagtcgc aagttgcggg attcgaaggc gtaatgcagg ctaaaccatc
     3181 ggataaggca ttcacgccgc atccgacatc ttttgcctga tgcttcgtat ttacgcccca
     3241 accaccttcg ccaccgcgtg ggcgaaacgt tgcatccctt catcgatatc cgcatcttcc
     3301 accaccagcg acggtgcaaa acgcatcaca tccggtccgg cattcagcac cattacgcca
     3361 gcccctgcgc ccgcatacag gaaatcacgc gcccgacctt tgaactgtgg cttaagctcc
     3421 gcgccaatca acagccccat accgcgaata tcgctaaaca catcgtactg ctgatcgatc
     3481 ttctgcaaat ggtcaacaaa acgctggcgt ttcgcctgaa tgccttccag cacttcaggg
     3541 gtattgatga tatcaaacgc cgcgcccgct actgcacagg ccagaggatt accgccgtag
     3601 gtggaaccgt gagaaccagg atgaaacgca gaagcaattt ccgccgtggt cagcatggcg
     3661 ctaatcggga agccgccgcc taacgctttc gcagaggtca gaatatccgg cgtcacgccg
     3721 tagtgcatgt aagcaaacaa atcgccggtc cgccccatcc cgcactgcac ttcatcaaac
     3781 accaataatg cctgatgttg atcgcacagc tcgcgcaagc cctgcaaaaa ctctggcgtc
     3841 gcagccgtca cgccgccctc gccctggatc ggctcaacca ccaccgcaca ggtgtgatca
     3901 tccatcactg ctttcactgc gtggagatcg ttaaagggaa cgtggatgat gtctgccggt
     3961 ttcggcccaa agccgtcgga atattttggc tgcccaccca ccgaaacggt aaacagcgag
     4021 cgaccatgaa aagcgttatg gaaggcaata attttggttt tgaacgggct atgacgcaca
     4081 caggcgtaat ggcgtgccag tttaaaggcg gtttcgttag cttccgtgcc ggagttcata
     4141 aacaccacgc gttcggcaaa cgttgcctca atcagtttac gcccaagacg cagcgccggt
     4201 tcattggtga aaacgttact gatatgccac agagtttcgc cctgggtttt taacgcgttc
     4261 accagcgcag gatggcaatg gcccaacgcc gtaactgcaa tgccacccgc gaaatcgaca
     4321 tactccttgc cttgctgatc ccagattcgg ctgccctgac cttttaccgg aatgaactct
     4381 gccggagcat aaatcggcag gatcacttca tcgaaggtcg cgcgtgtaat tgctgtttgt
     4441 tcaattgcca tctcatgatc accctgttac gcataaataa ttgtgaaatt ataatcacaa
     4501 aatatgcata aaaaatcact aaatggcaat cagaaatcag cgatgcagga aattagccag
     4561 cagttgatgt ccttgttcgc taagaatact ttctggatgg aactgcacac cttccagatc
     4621 ccactggcga tggcgaatcc ccataatctc gcgggtttcg ctccaggccg tcacgtcaaa
     4681 gcacgctggt aatgagtcag gttccaccac cagcgaatgg tagcgtgtca cggtaagtgg
     4741 atttgccagc ccccgaaata cgccctcacc gttatgtgta atcggcgagg ttttgccgtg
     4801 catgaccttt gcggcgcgca caactttacc gccaaatgcc tgcgccattg cctgatgacc
     4861 gaggcagacg ccaagaatcg gcaagcgccc ggcatagtgg cgaataacgt caagggagat
     4921 cccggcttca tctggcgtac aggggccagg tgagatgaca attttttgtg gtttaagggc
     4981 gtcgatatcc gccagcgtca acgcatcgtt gcgcttaacc agcacatccg cccccagttc
     5041 acaaaagtac tggtagaggt tccaggtaaa agaatcgtag ttatctataa gcaggatcat
     5101 ggcggctccg gtacaaaaga accgcgctat tttactcaga ttccccggct tcgcttacca
     5161 ctttgctaaa tatcatctgc agtgcggtga gatcccccat tgccccactc tgatttgcct
     5221 gattccaggc ctctttttcg ataccctgcc agctcagttg atatccggca tgaatcgcca
     5281 gttgctcgaa gaagatccgc tgtgccagac cacttcccac ccggaaggga tgcagcacgt
     5341 tgatttcaca atagtaatgc gccagccgct cgacgaactt cgctttctcc aggccaacca
     5401 gataaccttc ttcctccaga tcctgcatca gggcattgcc ctctttttcg atataagcaa
     5461 agtggcagaa cggcgtatca ccctgataaa tatcaacttc acgcagttgc cccgcccagt
     5521 cgaaaatatc ctgatacagc tggcgatgga tagtgcgcaa atgcggtaaa ccgcgcacca
     5581 gcggaccaag ctcaatggtc gcagcacgca gcgccgtcat ttcgtaagcg gcctgttcca
     5641 gccgctgctg ctggtggatg ttcagccggt tacgcatgat atcaaggcca ggataaagat
     5701 acggatcgcg cccttcgccg aatttatcgc tcatagtgcc tcctcagctc ttcaaggcgc
     5761 gctaaagcct ctgcggcagt aagagtgact aaaggcatct cgacgccttc aagacggcga
     5821 ctggcctgga aattacgatt acgctgaagc tcccagagac gggacttttg cttatcggtg
     5881 agtttcttca cttgatgcct ccctgaacgt gtccatttgc cacaagtata agcggcaaat
     5941 ccgggttacg ccgggagagc agaagcgcgg gcaagattgc ccgcgcgaga aatcattacg
     6001 gcaggacttt agcggaaagg ataactaccg gttttgacgg cacattctgg tacggaccaa
     6061 cgtcatgagt cggcacctgg gaaatcttat cggccacgtc catgcctttc accactttac
     6121 caaataccgc gtaaccgaaa tcacgctgac catggtcaag gaaggcgtta tcggcaacgt
     6181 tgataaagaa ctggctggtg gcgctgtctt tgtcagcggt acgcgccatc gcgatggtac
     6241 cacgcgtgtt gcgcaggccg ttatcggctt catttttgat tggcgggttt ggttttttct
     6301 gctgcatctg ctcggtgaaa ccgccgccct gaatcataaa gccaggaatg acgcggtgaa
     6361 aggtagtgtt gttataaaaa ccgctgttca cataatcgac aaagttttgc acagacactg
     6421 gcgctttttg tttatccagc tccagttcga tgttaccagc tgaggttgtc aacaatacgt
     6481 gcgggtcccc tttcgctgcc attgctgcgg gagaaagagc agaaagagcg aaaacagcag
     6541 ccatcgccgc cagggtcgat ttgaacatga gatttcctta acaaacagag cagaaaaaaa
     6601 gcaagtgaag agattctaaa tagccttcaa gaacaaggcc atccctttac ctaattttac
     6661 gtatctgaaa tatatgtaac ctgacgatca ctttttattc cgtgatcaaa atcacctctt
     6721 aaaatgcaat ttagcaaccg attgcaataa aacatttaaa cagatcacaa aatcacctaa
     6781 aatcgcccgc tcgcggcata tcccgctatg ccattttatt tttgaacgca ggccaatcat
     6841 gactaacagc aatcgcatca agctcacatg gattagcttt ctctcctacg cactgactgg
     6901 tgcgttggtt attgtcaccg ggatggtgat gggaaacatc gccgattatt tcaatctgcc
     6961 tgtttccagt atgagtaaca ccttcacctt cctcaacgcc ggcattttaa tctctatctt
     7021 cctcaacgcc tggctgatgg aaatcgtccc gttgaaaacg cagttacgtt ttggctttct
     7081 cctgatggtg ctggcggttg ccggtttgat gttcagccac agcctggcgc tgttctcggc
     7141 ggcgatgttc attctcgggg tggtcagcgg catcaccatg tcgattggta cattcctggt
     7201 aacacaaatg tatgaagggc gtcagcgcgg ttcccgcctg ttatttaccg actccttctt
     7261 cagtatggct gggatgattt tcccaatgat cgccgcgttt ctactggcgc gcagcattga
     7321 gtggtactgg gtttatgcct gcatcgggct ggtgtatgtc gctattttta ttctgacctt
     7381 cggctgtgag ttcccggcgc tgggcaaaca tgcgccaaaa acggatgctc cggtagagaa
     7441 agaaaagtgg gggatcggcg tactgtttct ctctgttgcg gcgctgtgct acatcctcgg
     7501 tcagttaggt tttatctcct gggtgcctga gtatgccaaa ggcctgggca tgagcctgaa
     7561 cgacgcgggc acgctggtga gtaacttctg gatgtcatac atggtcggca tgtgggcgtt
     7621 cagctttatt cttcgcttct ttgatttgca acgcattctg accgtactgg ctggtctggc
     7681 tgcgattctg atgtacgtct ttaacaccgg aacaccagca catatggcgt ggtcaattct
     7741 cgctctgggc ttcttctcca gcgcgatcta taccaccatc atcaccctgg gttcacagca
     7801 gaccaaagta ccgtcgccaa aactggttaa ctttgtcctg acctgcggaa ccatcggtac
     7861 tatgttgacc tttgtggtta ccggcccgat tgttgaacat agcggtccgc aggcggcact
     7921 gcttacagca aacggtctgt acgctgtcgt ctttgtgatg tgcttcctgt taggtttcgt
     7981 cagccgtcac cgtcagcata acaccctgac ctctcattaa ttgctaatgc cggacggcac
     8041 tatcgtcgtc cggccttttc ctctcttccc ccgctacgtg catctatttc tataaacccg
     8101 ttcattttgt ctattttttg cacaaacatg aaatatcaga caattccgtg acttaagaaa
     8161 atttatacaa atcagcaata taccccttaa ggagtatata aaggtgaatt tgatttacat
     8221 caataagcgg ggttgctgaa tcgttaaggt aggcggtaat agaaaagaaa tcgaggcaaa
     8281 aatgagcaaa gtcagactcg caattatcgg taacggtatg gtcggccatc gctttatcga
     8341 agatcttctt gataaatctg atgcggccaa ctttgatatt accgttttct gtgaagaacc
     8401 gcgcatcgct tatgaccgcg tacacctctc gtcttacttc tctcaccaca ccgccgaaga
     8461 gctgtcgctg gtgcgcgaag gcttctacga gaaacacggc atcaaagttc tggtcggcga
     8521 acgcgctatc accatcaacc gtcaggagaa ggtgattcac tccagcgccg gacgtaccgt
     8581 tttttatgac aagctgatca tggcaaccgg ttcctacccg tggatcccgc caatcaaagg
     8641 ttctgatact caggactgct ttgtctatcg caccattgaa gacctcaacg ccattgaatc
     8701 ctgcgcccgt cgcagcaagc gcggtgccgt tgttggtggc ggcctgttag gtctggaagc
     8761 cgctggcgcg ctgaaaaact taggtattga aacccacgtt atcgaatttg cccctatgct
     8821 gatggcggaa cagcttgatc agatgggtgg cgagcagctg cgtcgcaaaa tcgaaagtat
     8881 gggcgtgcgc gttcacacca gcaaaaacac ccttgagatt gtgcaggaag gtgtcgaagc
     8941 gcgtaaaacc atgcgttttg ccgacggcag cgaactggaa gtcgacttta tcgtcttctc
     9001 taccggtatc cgtccgcgcg ataagctggc aacccagtgt ggtctggacg ttgctccgcg
     9061 tgggggtatt gtcattaatg attcctgcca gacttccgat ccggatatct acgccatcgg
     9121 tgaatgcgca agctggaaca accgtgtatt tggtctggta gcaccaggct acaaaatggc
     9181 gcaggtcgcc gttgaccata ttctgggtag cgaaaacgcc tttgaaggtg ctgaccttag
     9241 cgccaagctg aaactgctgg gcgtagacgt aggcggtatt ggtgatgcgc acggtcgcac
     9301 gcctggcgca cgtagctacg tttacctcga cgaaagtaaa gagatctaca aacgcctgat
     9361 tgtcagcgaa gacaacaaaa ccctgctcgg tgcggtactg gtgggcgata ccagcgacta
     9421 cggtaacctg ctgcaactgg tgctgaacgc tatcgaactg ccggaaaacc cggattccct
     9481 gatcctgcca gcacactctg gtagcggcaa gccgtctatc ggtgttgata aactgccgga
     9541 cagcgcgcaa atctgctcct gcttcgacgt caccaaaggt gatctgattg ctgccatcaa
     9601 caaaggctgc cacaccgttg cggcgctgaa agctgaaacc aaagcgggta ctggctgcgg
     9661 tggctgtatc ccgctggtta ctcaggtact gaacgcggaa ctggcgaaac agggcatcga
     9721 agttaacaac aacctgtgcg aacactttgc ttattcgcgt caggaactgt tccatttgat
     9781 ccgcgttgaa ggcattaaaa ccttcgaaga actgctggcg aaacacggca aaggctacgg
     9841 ttgtgaagtt tgtaaaccaa ccgtcggttc gctgctggcc tcctgctgga acgaatacat
     9901 tctgaagccg gaacatactc cgctgcagga ttctaacgat aacttcctcg ctaacatcca
     9961 gaaagacggc acctactcgg tgatcccgcg ctctccgggc ggtgaaatca ccccggaagg
    10021 gctgatggcg gtaggtcgta tcgcgcgtga atttaatctc tacaccaaga tcactggctc
    10081 ccagcgtctg gcgatgtttg gcgcacagaa agacgatctg ccggaaatct ggcgtcagct
    10141 gattgaagct ggcttcgaaa ccggtcatgc ctatgcgaaa gcactgcgta tggcgaaaac
    10201 ctgcgtgggt agcacctggt gccgctacgg tgttggcgat agcgtcggcc tcggcgtgga
    10261 actggaaaac cgctacaaag gcatccgtac gccgcacaaa atgaagttcg gtgtctccgg
    10321 ctgtacccgt gaatgttcag aagctcaggg taaagacgtg ggtattatcg ccactgaaaa
    10381 aggctggaac ctgtatgttt gcggtaacgg cggcatgaaa ccgcgtcatg cggatctgct
    10441 ggcagcggat atcgatcgcg aaacgctgat caaatatctc gaccgcttca tgatgttcta
    10501 catccgtacc gccgacaaac tgacgcgtac cgcgccgtgg ttagaaaacc tcgaaggcgg
    10561 catcgattat ctgaaagcgg tgatcattga cgacaaactg gggctgaacg cacatctgga
    10621 agaagagatg gcgcgcctgc gtgaagcggt agtgtgtgag tggactgaaa cggtcaatac
    10681 accgtccgcg cagactcgct tcaaacactt catcaacagc gacaagcgtg acccgaacgt
    10741 acagatggtg ccagagcgcg aacagcaccg tccggcaacg ccgtatgaac gtatcccggt
    10801 aactctggtg gaggacaacg catgagccag tggaaagaca tctgcaaaat cgatgacatc
    10861 ctgcctgaaa ccggcgtctg cgcgctgtta ggtgacgagc aggtagcaat tttccgcccg
    10921 tatcacagcg atcaggtgtt tgcgatcagc aacatcgacc cgttcttcga gtccagcgtg
    10981 ctgtcacgcg ggctgattgc ggaacaccag ggcgagctgt gggtcgccag cccgctgaaa
    11041 aaacagcgtt ttcgcttaag tgatggcttg tgcatggaag acgaacagtt ttccgtcaag
    11101 cattacgaag cacgagtgaa agacggcgtg gtgcagctgc gcggttaatt gttttaacgg
    11161 gaggcgcaat gcctcccctt tttgcatggt cctgtaatga ccttcggtat attgcaggac
    11221 attttttaaa ctttttgttt tatttttttg tttttatttt ttaaaggata atcaaatgtt
    11281 tacagacact attaataagt gtgcggctaa cgctgcgcgc attgcacgcc tgtcggcaaa
    11341 taacccgctc ggcttttggg tcagctccgc catggcgggc gcgtatgtgg gtcttgggat
    11401 catcttgatt ttcacgctcg gtaatttgct cgacccgtcc gtacgtccgc tggtgatggg
    11461 cgcgaccttt ggtatcgcct taacgctggt gattatcgcc ggttctgaac tgttcaccgg
    11521 acacaccatg ttcctcacct ttggggtaaa agcgggcagc atcagccacg ggcaaatgtg
    11581 ggcaatcctg ccgcaaacct ggctgggtaa cctggtcggt tccgtcttcg ttgccatgct
    11641 ctatagctgg ggtggcggta gcctgctgcc ggtagatacc agcatcgttc actccgtcgc
    11701 gctggctaaa accactgcac cggcaatggt actgttcttc aaaggtgcat tgtgtaactg
    11761 gctggtttgc ctggctatct ggatggcgct gcgcactgaa ggggcggcga aatttatcgc
    11821 tatctggtgg tgtctgctgg catttatcgc gtccggctac gagcactcca tcgctaacat
    11881 gacgctgttc gcgctctcct ggttcggcaa ccacagcgaa gcctacacgc tggcgggtat
    11941 tggtcataac ctgctgtggg tgacgctggg taatacttta tcaggtgccg tattcatggg
    12001 attgggttat tggtatgcta cgccgaaagc gaatcgtccg gttgcggaca aatttaatca
    12061 aactgaaacg gctgccggtt aattactaag gggtttttac gtggatcatt tgcctatatt
    12121 ttgccaatta cgcgatcgcg actgtctgat tgtcggcggt ggtgatgtcg cggaacgcaa
    12181 agcaaggttg ctgttagacg caggcgctcg cttaacggtg aatgcattag cgtttattcc
    12241 acagttcacc gcatgggcag atgcaggcat gttaaccctc gtcgaagggc catttgatga
    12301 aagccttctc gacacctgct ggctggcgat tgcagcgacg gatgatgacg cgcttaacca
    12361 gcgcgtcagc gaagccgctg aagctcgtcg catcttctgt aacgtggtcg atgcgccgaa
    12421 agctgccagc ttcattatgc cgtcgattat tgaccgctca ccgctgatgg tggcggtttc
    12481 ctctggcggt acttctccgg ttctggcgcg cctgttgcgc gaaaaactcg aatcactgct
    12541 gccgctgcat ctgggccagg tagcgaaata cgccgggcag ttacgcggtc gggtaaaaca
    12601 acagttcgcc acgatgggtg aacgccgccg cttctgggaa aaactgttcg ttaacgaccg
    12661 tctggcgcag tcgctggcga acaacgatca gaaagccatt actgaaacca ccgaacagtt
    12721 aatcaacgaa ccgctcgacc atcgcggtga agtggtgctg gttggtgcag gtccgggcga
    12781 tgccgggctg ctgacgctga aaggactgca acaaattcag caggcagatg tggtggtcta
    12841 cgaccgtctg gtttctgacg atattatgaa tctggtacgc cgcgatgctg atcgcgtttt
    12901 cgtcggcaaa cgcgcgggat accactgtgt accgcaggaa gagattaacc agatcctgct
    12961 gcgggaagcg caaaaaggca aacgcgtggt gcggctgaaa ggtggcgatc cgtttatttt
    13021 tggccgtggt ggcgaagagc tggaaacact gtgcaacgcg ggtattccgt tctcggtggt
    13081 tccgggtatt accgcagctt ctggttgctc tgcctattcg ggtattccgc tcacgcatcg
    13141 cgattatgcc cagagcgtgc gcttaattac cggacactta aaaaccggtg gcgagctgga
    13201 ctgggaaaac ctggcggcag aaaaacagac gctggtgttc tatatggggc tgaatcaggc
    13261 tgcgactatt cagcaaaagc tgattgaaca cggtatgcct ggcgaaatgc cggtagcaat
    13321 tgtcgaaaac ggaacagcag tcacgcagcg cgtgattgac ggtacgctca cacaactggg
    13381 cgaactggcg cagcaaatga acagtccatc gctaattatt attggtcggg ttgttggcct
    13441 gcgcgataaa ctgaactggt tctccaacca ttaatttaat cagggccaga gaattctggc
    13501 ctttttcgtt tacaactctc tttatatatt cagcagataa aaatataact cattaacaaa
    13561 aatcatttgt ttttgccccg ttatattcac cgttacagcg acttacaaaa ttaagtcatt
    13621 cactcactga cgccgattaa atattcctgt atcgttctta atgagcagtc tacttatatt
    13681 catataatca atgaatatta atcaatatat aatacatagg gaatgtaaat gaacaaattt
    13741 attaaagttg cactggtagg tgcggtactg gctacgttaa ctgcatgtac tggtcatatt
    13801 gaaaaccgtg ataagaactg ctcttacgac tacctgctgc acccggcaat ttctatttct
    13861 aaaatcattg gcggttgcgg tcctactgca cagtaagtct ctgacagaaa tcggctaaca
    13921 ccggtttaat tgcccgacgc cctctggatt ttccgagggc gtatttgttt ctatgtcctt
    13981 cttgcccccg cccgtaaaaa tattttcact ttaaattcaa tttgataaat acatccatta
    14041 gccacagcgc gatcctgtgc gaaattttgt gatcttcctc cacattacat aacatcatat
    14101 gttgttatat tcatcatgca ttgtcatgtt accttttaaa tgactgcaaa ctctccccta
    14161 caacgtattg gacaagaaaa aggtatcgct atgggaagcc aggaactcca acgcaagctc
    14221 ggattttggg ccgttcttgc aatcgccgtc gggacaaccg tcggctccgg tatttttgta
    14281 tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc ttacggtgct cgcgtttgtc
    14341 attggcgggt taattgtgat cccgcaaatg tgcgtctatg cggaactatc caccgcttat
    14401 ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg gaagccgacc gctggctttc
    14461 ctctccggct gggccagctt ctgggccaac gatgcgccgt cattgtcgat tatggcgctg
    14521 gcgattgtca gcaatcttgg ctttttaacg cctatcgatc cgttgctcgg taaatttatc
    14581 gccgccggat taattatcgc ctttatgttg ctacacctgc gctccgttga aggcggcgca
    14641 gcgtttcaga cgctaattac catcgccaaa attatcccgt tcactatcgt cattggcctt
    14701 gggatcttct ggtttaaagc ggagaatttt gccgccccta ccaccactgc gattggcgca
    14761 acgggcagct ttatggcgct gctggcgggg atctctgcca ccagttggtc gtataccggc
    14821 atggcctcta tctgttatat gaccggcgaa attaaaaacc ccggaaaaac catgccacga
    14881 gcgctgattg gttcctgtct gctggttctg gtgctctaca ccctgctggc gctggtgatt
    14941 tccggcctga tgcccttcga caaactcgcc aattctgaaa cgccgatttc cgacgccctg
    15001 acctggatcc ccgcactcgg cagcaccgct gggatctttg ttgccatcac ggcgatgatc
    15061 gtcattcttg gttcgctttc cagctgcgtg atgtaccagc cgcggctgga atacgcgatg
    15121 gcgaaagaca acctgttctt taaatgcttc ggccatgtgc atccgaaata caacacgccg
    15181 gatgtctcca tcatcctgca aggggcgctg gggatcttct tcatcttcgt ttccgatctc
    15241 accagcctgc tgggttattt caccctggtg atgtgtttca aaaataccct caccttcggc
    15301 tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc tgtggcgtac tccggctttc
    15361 gggctgatga ccaccctcgc cattgcgtca agcctcattc tggtcgcctc aacctttgtc
    15421 tgggcaccga ttcccggcct tatctgcgcc gtcatcgtta ttgctactgg tctgcctgct
    15481 tacgccttct gggcgaagcg tagccgccag ctcaacgctt tgtcgtaatg ttatctggag
    15541 aaaataaaat gttggatatt gataaaagca ccgtggactt tctggtcacc gaaaatatgg
    15601 ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct ggtgcacacc atcgtggaag
    15661 agatggtgaa gcgcgacatt gatcgtattt atttcgttgc ctgcggatcg ccactcaacg
    15721 cggcgcaaac ggcgaaacat ctggcggatc gcttttccga tcttcaggtc tacgccattt
    15781 ccggctggga gttctgcgat aacaccccgt atcgcctcga cgatcgttgc gcggtgattg
    15841 gcgtttctga ctacggtaaa acggaagagg taatcaaagc gctggagctg ggccgggcct
    15901 gcggcgcact cactgcggcg ttcaccaaac gcgcggacag cccgattacc tcggcggcgg
    15961 aatttagcat tgattatcag gccgactgta tctgggaaat tcacctgctg ctctgctaca
    16021 gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc ggaaatcggc aagatcaaaa
    16081 acgatctcaa gcaattgccg aatgcgctcg gtcatctggt acgcacctgg gaagaaaaag
    16141 gccgccagct tggtgaactg gccagccagt ggccgatgat ttataccgtt gctgcgggtc
    16201 cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct gatggaattt acctggacgc
    16261 acggctgcgt gattgagagc ggagagttcc gccatggccc gctggagatt gtcgaaccgg
    16321 gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg ccacaccacc gaacgcgcca
    16381 ttaactttgt taaacagcgt actgacaacg tgatcgtcat cgattacgcc gaaatttcgc
    16441 aagggctgca cccgtggctg gcaccgttcc tgatgttcgt gccaatggag tggctctgct
    16501 actacctgtc tatttacaaa gatcacaacc cggatgaacg ccgctattac ggtggtctgg
    16561 tggaatatta atccctctcc cggcccggca acgggccgaa tttacgcaag gagttacccg
    16621 atgaaaacag gtatgtttac ctgcggccac cagcggctgc ctattgaaca cgcatttcgt
    16681 gatgcaagcg agctgggtta cgacggcatc gaaatttggg gcggtcgccc gcacgcgttc
    16741 gcgccggact taaaagcggg cggcatcaaa caaatcaagg cgctggcgca gacgtatcag
    16801 atgccgatta tcggctatac gccagaaacc aacggctatc cgtataacat gatgctgggc
    16861 gatgaacata tgcgtcgcga aagcctcgac atgatcaagc tggcgatgga tatggcaaaa
    16921 gagatgaacg cgggttatac gctgatttcc gcggcccacg cgggctatct cacgccacct
    16981 aatgttatct ggggacggct ggcagagaac ctaagcgaac tgtgtgagta cgcggaaaac
    17041 atcggcatgg atctaatcct cgaaccgtta acgccgtatg aatcgaacgt cgtgtgtaat
    17101 gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc cgcgcctgtt cagcatggtc
    17161 gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga gttatttcga caaactgggc
    17221 gataaattac gtcatctgca tattgtcgac agcgacgggg ccagcgacac gcattacatt
    17281 cctggagaag gcaaaatgcc gctgagggaa ctgatgcgcg atattatcga ccggggctat
    17341 gagggttact gtacggtgga gctggtgacg atgtatatga acgagcccag actctatgcc
    17401 cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg atgagagatg aaaaccctgg
    17461 cgacaatcgg cgataactgc gtcgatatct acccgcaact gaataaagcg ttttctggcg
    17521 gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg catacagccg ggatgcatta
    17581 cctgggtggg tgacgatgac tacggcacaa agctgaagca ggatctcgcc cgcatgggcg
    17641 tcgatatcag ccatgtccat acgaaacacg gcgttaccgc acaaactcag gtggaactgc
    17701 acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt gatggccgac tttgccctga
    17761 gtgaagagga ttacgcctgg ctggcgcagt atgacattgt gcacgcggca atctggggac
    17821 atgcggaaga cgcattccca cagctgcacg ctgcgggcaa acttaccgct ttcgacttct
    17881 ccgacaagtg ggacagctcg ctctggcaga cactggtgcc gcatctcgat tttgcctttg
    17941 cctccgcacc gcaagaagac gaagcgctgc gtctgaagat gaaagcgatt gttgcccgtg
    18001 gcgcaggcgt ggtgattgtc acgctgggtg aaaacggcag cattgcctgg gatggcgcgc
    18061 agttctggcg tcaggctcct gaaccggtga cggttatcga caccatgggt gccggagatt
    18121 cgttcattgc cggattcctt tgcggctggt ctgcggggat gacattaccg caggcgatgg
    18181 cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca cggtgcctgg taggtataac
    18241 gttggcgtga gcatcttcac gccaacgtgc tgttacttgc cggaaaacga ccctataatc
    18301 cgagtaattc attctttatt tcagggtcga ttatgtcagc tacggaccgc tactctcatc
    18361 aactcctcta cgctaccgtc cgccagcgac tgctggatga tatcgcgcag ggggtttacc
    18421 aggccgggca acagatccct accgaaaacg agctttgtac acaatataac gtcagccgca
    18481 ttaccattcg caaagccatc agcgacttag tggcagacgg cgtactgatc cgctggcagg
    18541 gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc cctgcttact gtcagtggtt
    18601 ttaccgattt tggcgtctca caaggcaagg cgacgaaaga gaaagtgatc gaacaggaac
    18661 gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc cggaaacagc gaagtgttcc
    18721 atctctgccg ggtgatgtat ctcgataaag agccgctgtt tattgatagt tcatggatcc
    18781 cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga aggaagctcc acctatcagt
    18841 tatttcagga gcgttttgac acgcgagtgg tcagcgacaa aaagaccatc gatatctttg
    18901 ccgccacccg cccgcaggca aaatggctga aatgcgaact gggcgaaccg ttgtttcgca
    18961 tcagcaaaat cgcctttgat cagaatgaca aaccggtgca cgtctccgaa ctcttctgcc
    19021 gcgccaatcg catcacctta actattgata ataaaagaca ttaaccgtag gccggataag
    19081 atgcgccagc atcgcatccg gcgatgctgg cgcgttgaat tttacatccc gtacgttccc
    19141 ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt cagtacggaa tttagcttga
    19201 cggtgctatc cagcattaaa tactggcaat actctcacgc aaaatccgca gcaccgtctc
    19261 ttcaccgctg cgattcgggt taatgcgaat cgcacaatgt tctgattgtg gattcgcctg
    19321 gcgaaacgtt ccggaaaggc gataaaagag cggcgggatt tcatatttcg actctgcacc
    19381 caccgggtaa ggcaaggcac cgcgcttttg cgcctcttcc agcactctgg cggcaatcgg
    19441 ctgatgaaac tcgacaatca acaccttcga ctgcgcatta gcaatcaccg cgcttttcac
    19501 ttccggcacc gcaccaccgt taagcaaagc cagcaaccgt tcagataccc ctgcctgcac
    19561 cgcgtgcatc actggcgcaa acaccagacc acgcaatact tccagcgcct gtgcgccctg
    19621 gatctggcta ccgccggagt aaagcgtggc gcgaatacgg ttgataacat cagcatcgcc
    19681 gaccactgca ccaacaccct ctggcccaaa tagcttgaag caggaaaatg tcgagacatt
    19741 cgcgccgcat tcacagccga ttcgcgccac cttcatcacc gcatagttgt catcggttaa
    19801 cgctggaaca cctgccgcgc gcaacgttgc cagcacatct gccagcacgt agctgtcctg
    19861 cggctgctgg cgcgtatgct gcaccagcgc cgcatccggt tgttgctcgt cgacgacctg
    19921 cttcagtgcc gacaggtcat tgaaatcaac agtaataagc gtcagcccca tctgctcaat
    19981 aataacccgt gtcgtcgggt aaacaggcgc gtcatgcacc agaagacgct gccccggttt
    20041 gagcaaagcc gccagcccgg cgcgaatcgc gccagtcccc gcgccctgca ccagcgccgc
    20101 agcctgtgcg tgaaatgcat cagccagcac ctgttccaca cgctgggtaa cgcgcggttg
    20161 attcagtcct ggcgttaagc ctaaatcacc gccagtaaga aactcgctgc cggggaaatg
    20221 gcgacaaatg ctatccacca gcgcaaactg cttttgctgc gcctcaataa tcgtcaggct
    20281 ttgcagagga aacgtcttca taggtgcctt ccttaagccg gaacgaacag gccaagccag
    20341 taaagaacat tcagcagaat accggtgatc attactgcca cgaccggtgc cgccattttc
    20401 tgtaccggac ggcccaggga ttcgttgagg aagtaaatgg caaccgcgat agagaatccg
    20461 gtataacccg ccatcttaat tgctgcgaaa atcgaaccga ccagcagcgc cacttccatc
    20521 agcatattca tggcgttacg gatgttatcc gacgcattac gcaccgacgg gtagcgtccc
    20581 agccatttgc cgatcgaacg aagcagcaag acttccgccg aaataaccac tgcgcctaat
    20641 accgctgcaa ccatcggatt cggcgagaga tagcccaccg cataaacaaa ggtaaagccc
    20701 gcaactgcat acacacccgt tgctaaggcg gtggtggcaa tcaacggtac aaaacccagc
    20761 ccgcgcataa attctgccag tgccgcctgg ttgatcagcg tttgcgattg ttccggcgtt
    20821 acgcctgcgg agtacgcttt ctccagagtg aagatcgaca cttcactgcc ggcgaaaatc
    20881 ttcatgctgg caacggcggc aatcaatgct cccacgatgg cgatataggg taagtttttg
    20941 ataatccgcg acgtgcgttc ttcaaacacc gaaagcccgc tggcatcaat atcattttca
    21001 tcacgatgac gcaggtcgtg agttatcgcg atccccagca gcatcaccat gccaataaag
    21061 atttcgatgg attcagggtt aagatgtggg aaatagcgca cgactaccac acgggtcatc
    21121 agtaccacca cggcggcgat cagactttgc ttccagccaa actggtagaa aatcgccacc
    21181 agcgggaaca gtgcaaaagc tgaaaccacc ggcgagctta attcccccag gctacctaat
    21241 acatccaccg gcagcgcggt cagcagctgg tttactggca acaggcaagt aaggatcaac
    21301 acgccccaga tagcgccaag accaaacgcc atcaggctgt ttatcgccag gacgccgagg
    21361 atatcggtag gaagaaagag taaccatgcg ttgagcagcc cggttttcag ggtaaaagag
    21421 atccccaccg aggccacaaa accaatgctc agaccaaaag cgatactccc cgcctcgcga
    21481 cggttcatat agccttcaat cagttgcggc aggatcgggc ggatgccgtc atgaaaaaca
    21541 gccgccgagc gatgcgccag aagcgatgtc atacccgtca ggcacgccac cacgataatc
    21601 tgaatataca gatccatagt cgtgcctttt atttcagatg gttaatcagc atcggaatcg
    21661 cgtgttcgac gtgctcaacg gaaaggccaa acgccacttt tccttcagcg atcattttgg
    21721 cgatatgctc gtctttcgct ttaatgcctg gtttggcaat ggtgcaactt ttgttatagc
    21781 caatcaccgc gatggcaatc gacaatgccg cgcccgcacc ggtattacac gcaccgatgt
    21841 agtaatccag ttgcccggat ttcactttca ttgccgcttc catgtcgttg tgaatgaaaa
    21901 cttcaaaact gccaggagcc gtcgcttcaa tggttttttt aatctgctca cgctgtaagc
    21961 ctgcaacgcc aatctttttc atgagtctgt ccttattgga aaaattgaga gggagtttca
    22021 cgtaacatca catccacatc ggcctgactg aatcctgact ggcgcaattg cggaataaag
    22081 gtggtcaata aatagtcata gccataacca ccattggctt ttaaatggga gcggcgcgta
    22141 atatccatcg acagcatgac gcggttcagc aacccacggt cacgtagcgc atgaagcatc
    22201 gcaatacgct tttcgtccgg gtagtaactg ttcttaccaa tggtgtcgaa ctgcacgtac
    22261 gcgccgagat cgatcatctt caaaatgttg tcgaggttgt ctttcagatc gcagtgacca
    22321 acggtgacgc gcgaaagatc aaccccgtgg gcttgtagca acgccagttg ctccagcccc
    22381 atcgtgctga acgacgtatg cgtggagatc gggcgtccgg tctggttatg cgccagcgca
    22441 gcggcaataa ataccttctc ttccagcggc gtaatctttc cttcgctggt gccgatctcc
    22501 gcgatgatcc cggctttcag ctccgtgcca tcgataccct gttcaatttc atcgaccatc
    22561 tcctgcgcca gttcctgcac gctgcgggtc accacatgtt ccgggaaaaa cgcgtcctgg
    22621 taataaccgg tacaggccac cacgttgatc cccgtctcgc gcattacatc aagcataaat
    22681 tgcgcattgc gccccatgta acggttggtc atctcaatca cattacgcac gccccgggtc
    22741 atcaggtcgt tcatctcctg gcaaatgaac gcatactgat caaggcggca gtccacgttg
    22801 tttttaaagc cggagagatc aatatgcaga tgctcatggg cgagggtgta acccgtcgga
    22861 tcaaaactca tagggtgtct cctgcaaacc ggagggagga aagaaaagag cgaccatttt
    22921 gtggcggtgg cgcgcggaaa aattcacaca cggtagcccc cacatcagaa agcgtggtgc
    22981 gcacaccgag ctgcgtagcg atcatccctt gctgataaac cagcactggc accacttcgc
    23041 gggtatggtg gctgtgacca atggtcggat cgttgccgtg atccgccatc acgaccaggc
    23101 aatcatctgg ctgcatcgcc tcaacaagcc gggcaaggtt acggtcaacg acctgcaaac
    23161 gttcggcata acgtgcgacg tcttctgcat gaccagcgag gtcggtttcc tgaatgttgg
    23221 tgcaaataaa cgccgtcgga tgggtgttaa attcgttgag ggtgatatcc ataatccgct
    23281 ggctatccac cagattttgc cagctcacgc cataaggatt gttgacgata tctgccacct
    23341 tacccaccag cacggttggc acgcctgctt catacagttt ttgtggcacc tgcacttttt
    23401 tatcgacgcc atagcccata tgcacgacct ggaaaccgtt gtcataagcg ccagaacgcg
    23461 gcgcattgat accaataaag cgcccttctt tgctttctgc ggcatcgaga atgcgttgac
    23521 tgtcggttaa caggccacca aatgtaatga cccgaccgac ctgtacctgc tcacgcacga
    23581 tacgaccaat tttgattgcg tcgtcaaaag agatcacaga gagattggcg gtaatgttat
    23641 agacctggcc taaatccgcc tcgagattat cgccaatcgc aaccgcctga ttgacccaca
    23701 gaaattgcag atcatcgcca cggcgctcca cctgccagcc agcggaaact aatgcctgct
    23761 caacacggcc aatcacatcg cgaaagggca tccgcagcgg cggtaacggg cgcgtgccta
    23821 aaatttcctg atgccccata aaggtatcgc caccttcatg ttgcagctct gccacccccc
    23881 aggttgcgga atctgacggt tgcatatcac ctggtgcata acccaatgcg ttgattagcc
    23941 ccagcttctc cagcgctggt agctgcaaat gcggcaactg gctcaggatg tgaccacagg
    24001 tattcgcccc ggcatcttgc gggcgcacca gcgtgacatc tttcattgcc cctacgccaa
    24061 agctatcaat cactaacacc acaaatcgcg ccattacgcc cccagagagt ttccaagact
    24121 gtcataacga ccgacgattt ctggttcgcc acggtgaata ccggacacca gcaccacatc
    24181 gctacgagtg acaaaaatct gcgtgcggaa acagagcacc actgcactgc tcaccggata
    24241 ctcgcctgcc agcggcaggg tgtagtcgat actgctgtca tccacagttt tgagattggt
    24301 ttcagtaatc ttttgatttt ctggcgtaaa caccagcgca tgttgcgcat gaccacgacg
    24361 gtagtaaccg ccgccgtagc agtagctgtc gccacggaaa tgatgggaga tttcacttaa
    24421 ccagagcatc gcgatacgtt caggctgatc gccctgctgg tttgccggaa tagtgcccgt
    24481 cagcgcatga ccgggttcgg catgagtcac accgtattcc gccagcaatg gcagcgaagt
    24541 gcagctggtc gctgaaggcg cgttcagttg ctcaagtgca ataccagatt tcgccagttg
    24601 atcccgtgcc tgtatcagcg tgtgaagatt cggtgtcggc aaaacttttc cggcagcctc
    24661 atcccaaagc aggcaaggga aatgggtaag tccggctaaa tgcagccccg gcagtttctt
    24721 aatttcagca accacctcgt gcagcgaatg ctgtacaaaa ccgctctcct gccccggata
    24781 aagaaaatca tcgtcgctat aaactttgag cagcacaaac tgcacacgtc cggccttcac
    24841 cgctgccgca gaaacttccc gcgctttgtc gagagtaaac acggtgatga cgtcggtgcc
    24901 ctgttcaacg gcgtcagcaa cctgatgaca agggatttgt accagatgcc cctgatgcgc
    24961 caccgacaaa ccagcgcggc gcatgactcg cgcctctttg taatccaccg ccacaatgcc
    25021 gctgtaacct aatgccagca atttttccgc cagccatggg ttacggccaa actgcttggt
    25081 catcagatac agttcaatgc cgtaaagccg cgccgtctca atcagtcgct taccgttttc
    25141 cagtacctga tccacgtcga tcacccagct gtccggggcg atcttgccct gctgccacaa
    25201 acttagtgcg gcggaaatca gcgccgggtt ctggcgtttc aatgcttcta caaacatctt
    25261 gttgcctcgt ttctgttcct tgaattaaat attcattttt ttgaatattt aggttgtgcg
    25321 acgtggattt gtcgggagct tcaaacctct tcgttagcag ccatccataa tccataaagg
    25381 ttggccagca aatagccttc ttcacacggg ttaacttcca gtgcgaattc cttcaacaac
    25441 acctgatgca attgcacaac ggcttgccag tggctggatt gcgccagctc tgccagcaat
    25501 tcgttatcca gcggctctat ttcttcacca cggcgactgc gcatcagtgc actcgccata
    25561 tgtgtcatcg ccatcgttcc ttgctcactg cgcaccggca gatggcactc tgtttccagt
    25621 acgttgacga cctgcatcat gcctttgcag atgtccttat caataacgcc tgcctcgcaa
    25681 agcaggttga gtctggtttc catatcagat gcctttcgtt taataactcg gttcctgttc
    25741 cccactcact acccgttgtt gatgggcgag cagggcgtgt ttatctacaa cggcacgcag
    25801 cagttgttgc attgggtaat catcgactcg cgtcaggacc acggcttcgg tggcctgtaa
    25861 aaatcgcgga tcgtctgtga gcggcgtcgc ctctaatccc agcatggtca gttcgttttc
    25921 cgccaccacg ttccagatca ccgcatcgac atcgccttta acaatgcgtt gtaaactctc
    25981 gtgataagag agatcgactc gttccacatc actaccgcca aaaaaaacat cggtcatgat
    26041 tttctgatcc gccgaacggt tatccagccc cacgcgcttc acgtttgcgg actcgccttt
    26101 acggcaaatc aactggtgct cgccaacgta ggtgtgcggc cccaactcca gcgcgaggca
    26161 taaccctttt tgcgtgagat aactttccgc cgccagtcgg gaaaccaccg ccatgtcata
    26221 cacgccgtta agtaaacact ccacgcgaat atccgcgccg cgcatatgcg catagtaaaa
    26281 aggaatgcca tcaaactgag ctttcagccc gctcgctaaa ccttcgtaca agcgggtata
    26341 gggcaaaggc atcgcacata ccacgttgtt gatatccaca tgactcagca atgctttgtt
    26401 atccatctcg accagataac tgccattgca cccacggcgt tcaatccgta tcgctccgct
    26461 tgattccagc gttttcaacg cggcctgcgt caggccaacc gatgaacggc attcagttgc
    26521 cagctcatct atggttttca acctattacc gcatttttca cccaacaaat aacgggccag
    26581 cgtcgtgata acgacgcctt cttttttgat aaacgttcga cgcataatat attttcaata
    26641 aaatgaatgt ttatatcttc attaaaatga agataagtgg caactgtgag atgtggaaac
    26701 ggcgaggcgc ttcacatttt ttcggattat tgcaaatgag gataaaaaaa ccgggtttcc
    26761 ccggtttcag agtgatgacg aagtgattta ttcgacaatg cgagcaaagg gtaattccca
    26821 ctggcgcttg cctgctcccc tcaccctaac cctctcccca aaggggcgag gggaccgtcc
    26881 gagcgttttg atattacggc ttcgccacaa aaccaatcgc ttcgtacacc gctttcagcg
    26941 tacgggaagc gtgtacactg gctttttctg cgccgtcttt catcacctgt tgcaggaagg
    27001 cttcatcgtt gcggaaacgg tgatagcgtt cctgcaattc agtcagcata ccggaaacgg
    27061 catcagccac ttcacctttc agatgaccat acatcttgcc ttcgaactgt ttttccagtt
    27121 ctgggatgct ctggcccgtt accgcagaaa ggatatccag caggttggaa acgcccgctt
    27181 tgttctgcac atcgtagcga actaccggcg gctcgtcgga gtcagtgacc gcgcgtttga
    27241 ttttcttcac taccgatttc ggatcttcca gcaggccgat aacgttattg cggttatcgt
    27301 cagacttgga catcttcttg gtcggctcca gcagcgacat tacgcgcgcg ccagatttcg
    27361 gaataaacgg ctccggcacc ttaaagatct cgccatacag cgcgttgaaa cgctgggcga
    27421 tatcgcgact cagttccagg tgctgtttct ggtcttcacc caccggtacc agattagttt
    27481 gatacagcag gatgtccgca gccatcagca ccggatagtc aaacagacca gcgttgatgt
    27541 tctcggcata acgcgcagat ttatctttaa actgggtcat gcggctcagt tcgccgaagt
    27601 aggtatagca gttcagtgcc cagcctaact gcgcatgttc tggtacgtgg gactgaacaa
    27661 aaatggtgct tttctcagga tcgataccac aagccagata caaggccagc gtatccagcg
    27721 tcgctttacg cagcttctgt gcatcctggc gcacggtgat cgcgtgttgg tcaacgatac
    27781 agtaaatgca atggtagtca tcctgcatgt ttacccactg acgcagcgca cccatgtagt
    27841 taccaatggt caattcacct gagggctgtg cgccactaaa aacgatgggc ttagtcattt
    27901 ttcgattcct gattttcgct atgcggaagc cctaatgcgg gcagaaggtc atttatagac
    27961 tgataaatta catcaggctg gctgagatcg atagcctcgc cgtagttata tccgtaggtt
    28021 aagccaactg atgggcaacc tgccgctttt gccgcctgaa tatcattgcg tgagtcgccg
    28081 acaaacagca tctcttgtgg ggcaattccc atccgctcag ccaccagtaa cagcgggtcc
    28141 ggatgcggtt ttttgttttg cacatcatcg ccgccgataa ccacgctaaa gtatttggca
    28201 atatctaagg cttcgagcag cggcgcgacg aacggcgtcg gtttgttggt gaccaggcct
    28261 agcggcaggc ctttagcctg caacgcgccc aacgtatcgg caacgtgcgg gaacaaaaac
    28321 gtcccctctt cggcaacctc gctatagtag cgatcgaaca gtttacgcag aatacgtacc
    28381 tgttcttctg ccggaatgtc gtcatcaacg ggcggtttac ccattgtttt acgcagagtc
    28441 gcacgttcct gacgcgccca ggtcaatgcg cgctccatca gaacatctgc gccgttacca
    28501 atccaggtaa taacgcgttc ttcacctgcg atgggcaact ccagcgcata cagcgccata
    28561 tctaccgcag cagcaagacc gggagcactg tcgaccagcg taccatcaag atcaaaagcg
    28621 acgccgcgaa tatcttcaaa cttattcatg acttaccttt gccagttcac tgcgcatttc
    28681 atcaatgact tttttgtagt ctggctggtc gaagattgcc gaaccggcga cgaacatatc
    28741 cgcgcccgcc gcagcgattt cgccaatgtt gttcaccttc acgccaccgt ccacttccag
    28801 acgaatgtca aagccagact cgtcgatacg gcgacgtact tcgcgcagtt tatccagtgt
    28861 ttgaggaatg aaagactgac cgccgaaacc aggattgacg gacatcagca ggatcacatc
    28921 cagcttatcc atcacgtaat ccagatagct cagaggtgtc gccgggttaa ataccagacc
    28981 cgctttacag ccattttctt taatcagttg cagcgtgcgg tcaacatgct cggaggcttc
    29041 tggatgaaag gtaatgatgc tggcaccagc ggcagcgaaa tcaggcacaa tgcgatcgac
    29101 gggtttcacc atcagatgta cgtcgatagg ggcggtaatg ccatagttac gcaaggattt
    29161 cagcaccatt ggcccaatcg tcagattggg aacatagtgg ttatccatga cgtcaaaatg
    29221 cacgacatca gcgccagctg ccagggcttt tgcggtatct tcacccaggc gggcaaaatc
    29281 agccgacaga attgaggggg caatcaaata ctgtttcatc cgcttctcct tgagaattat
    29341 tttttcgcgg gtgaaacgac tcctggtttg tacaaagcca gcagttcgtc caccttttta
    29401 cgtgtgccgc cgttgctgct tatactgcgt cgaactttga cgacatgcaa ttttgcgcgc
    29461 tgataccact cacgcgttaa catcgtatcg tgattggaga tcagcactgg aatatggcgc
    29521 tcaaccagac cttcggcgat ctccgccaga tgcgcttgtt gttcaagcgt aaaactgttt
    29581 gtgtgatacg ccgtaaagtt ggcggtcgca gacagcggtg cataaggcgg atcgcaatag
    29641 acgacggatg catcatctgc gcgcgccatg ctatcggcgt aagactcaca atagaaaaag
    29701 gcattctgcg ctttttcagc gaagtgatac aactctgctt ccgggaaata gggttttttg
    29761 tagcggccaa acggcacatt aaactcaccg cgcagattgt aacgacacag gccgttgtaa
    29821 ccgtagcggt tcaaatataa aaacagtacc gcccgacgga acggatcctg gcttttgttg
    29881 aactcttcgc ggaactgata gtaaacctcg gcgcaatttg tttcgggaac aaacagctcg
    29941 cgtgcggcct gtacgtactc atcagtacgc atcttcacaa tgttatagag actgatcagg
    30001 tcgctattga tatcggcaag gatataacga gaaaagtcgg tgttgagaaa caccgacccg
    30061 gcacctacaa aaggctcaac cagacattcg cccttgggca aatgccgttt aatatcatca
    30121 agcaggggat acttgccccc tgcccacttc aaaaaagcgc gatttttctt catgctgact
    30181 aactaattac accttctccg gctgtggaga aagctccgac agcatccagc gcttcaggca
    30241 actcccgcaa cgataaacca ttgcgggaga taaccttgat tacttcagat cggcctgtac
    30301 ctgacgcagc ggtttcgccc acgggttttt ggcctggaca tctgctggca atgtagatac
    30361 cgcttttttc gcctcttctt tcgaagcgta cacgccagaa accaggacat accacggctg
    30421 accattacgc gtcgtttcat agacaacgta gtttttcaga ttctctttct tcgcccaacc
    30481 gttcaggttg tcgtagttag aggaactgct cagctgcaga gtgtaatggc tggacggtgc
    30541 cgatttcaac gaacccacat tacctgcggt cttccctcca gcggctggtg ttgccgtggt
    30601 ttgcgccggg gatgccgtct gtactggagc cgtagtcgcc gtctcttttg gtgctggcgt
    30661 agaagtcgca gccggtgcct tcgtgctcgc cacaggagca gctggttcag tacgcttcgg
    30721 cgtctgtgct accggcttcg gttccgtttt cacggttgct tgcggttttt tcggttcaat
    30781 caccgcctgc tgacgagctg gacgcgtagt ggccggacgt tcagcagttt gcgttttcgc
    30841 cgtgtcacgc gatgcattgc cattgcgtac tggcgcgaca gttgctggtt cagtcggcaa
    30901 tgtagaattg accgccacat tgttcaactg ttgctgattt tgtggctggg tcagcgcatt
    30961 gttcaggtca ccctgcactt caacacgttg ttgaccatcc gttgccgccg gggtttgccc
    31021 ttgagtcggc gtagaagaga tcggtggcag agaaacatcc tgctgagtat tttccgcaga
    31081 cgtggttccc ggcgctggct gcaccccatt cgcctgatcg gtcgcattgc ctgcaagatc
    31141 aatactcttc tcgccagacg cggtttgatc gctggaagag gtcgaggggg cttttagcgc
    31201 agaaccgata ccgatgatca acagcagtag aaccaaaatg ccgacgccca tcatcatata
    31261 ctgacgagaa gcgggtttac tggctgcttt tttgcgctta cgcggacgac gctctacgcg
    31321 ctcttcatcc acggtttcat cttcggattc gtcaatttct tcttcgattt ccggttcctc
    31381 attgcgctct ttttgcgcac gagtcggacg gcgatcgtca gtgtcatcaa gttcaatatc
    31441 atcaaaattg atctgcggtt cgccacgttc agtacgctca gaacgttcag aagattgacg
    31501 agaacgacca gtacgacgat cgctgggatc gggtttcagc tcgtcttctg gtttgaattc
    31561 atccatttaa caccccacta aaaggttaat gcttaccacg ttgcaattaa cctgaagcta
    31621 atagaccgct tgataagcgg cctgaccttt cttgttgtta cgctgattga caatcggcaa
    31681 tggcgttaag aacaagctcg tgcgaaacgc cgctgcgaac ttcactctta ccaattgcca
    31741 acggaagaat taagcgtatc tctcccgcaa ggactttctt gtcacgcagc atatgcggta
    31801 aatacgcctg cgcggacatt tcgcgcggcc cattgaccgg taacccagcc cgcttgagca
    31861 gggttataat acgctgcgtt tcggcagaac taaactgccc gagacgttcc gacgtccgcg
    31921 ccgccatcac catacccgca gcgaccgctt caccatgtaa ccaattgcca taccccattt
    31981 cagcttcaat ggcatgacca aaggtgtgtc ccagattcag taaagcacgt aacccggttt
    32041 cgcgctcgtc ggcggcgaca acttctgcct tcagttcaca acaacggcga atacagtacg
    32101 ccattgccgg accgtccaga cgcaacaacg catccagatt ctcttccagc cagttaaaaa
    32161 acgcaccgtc aagaataatg ccgtatttga tgacttctgc cagccccgac gctaactcac
    32221 gcgggggaag cgttttcaga cagtcgagat ccaccaccac tgaagcaggc tggtagaacg
    32281 cgccaatcat gtttttaccg aggggatggt tgaccgcagt tttgccgcca acggaggaat
    32341 cgacctgcga cagtaacgtc gtcgggactt gaatgaaacg aacaccgcgc tgataactcg
    32401 ccgccgcgaa gccggtcaga tcgcccacta cgccgccgcc aagcgccacc agcgtagtat
    32461 cgcgaccatg cggcttttgt aacaacgccg taaagacggt atcgagtaca gccaggcttt
    32521 tatactgctc gccgtcaggg aggataacgc tatcgacgtt aacacccgcc tgttcaagta
    32581 cgccgcggac cttatcgaga tacagaggag ccagggtttc gttggtgacc aacatgacct
    32641 gctcgcccga tttcagcggt aagaatgaag ctggttcatt aaacaaacca gatgcgatgg
    32701 taattgggta actacgttcc ccgagagtaa cgacaatcct ctccataacg cgacatccac
    32761 cttaattact gtacccgcag acgagtgtat ataaagccag aattagttgc tttccagcat
    32821 gtgaataatc tggtttgcaa ccactttagc gctttgatca tcagtacgaa tggtcacgtc
    32881 ggcaatctct tcatacagcg gattgcgttc attggccaac gcttccagaa cttcacgcgg
    32941 cggtgtttca acgtgcagca acgggcgttt tttatcacgc tgcgtgcgtg caagttgctt
    33001 ttcgatggtc gtttcaagat aaacgacaac gccacgagcg gaaagacggt tacgcgtttc
    33061 acgggatttc acagagccgc cgccagtagc cagcacaata ccctgtttct cggtcaactc
    33121 attgatgacc ttttcttcgc gatcgcggaa gccttcttcg ccttctaaat cgaaaaccca
    33181 gcccacatca gctccggttc gtttctcaat ctcttgatcg gaatcgtaaa attccatatt
    33241 gagttgttga gctaactggc gcccaatagt gctttttccg gcacccatag gcccaaccag
    33301 aaagatattg cgtttctctg ccattttttc ggtactacta agactattcg ttaatgataa
    33361 acccgcttcg ctcagagagc gccgcaggac atgaactgaa acctcataag atattgcgag
    33421 agtcagactg aaaattatct caatactcca gcgggtttgg caactgaata aatcacccat
    33481 ccctctgcat atctggtcgc tgcaagcgcg ctgccttgct accaccgctc tggcgataaa
    33541 tcaccgggta agattagcgt aaaaaagaca gcaaaatgcc gcctgaatga taaatcatca
    33601 tcatggaacg caggcagcga agagcaccaa ctctcaaatc ggtactcctt gtatgctaaa
    33661 tacctgcgcg cgtcaaatag atgaaacacg ttcagcataa aaacattacc gcttttacgg
    33721 ctgtttactc actggaaacc agtcgtggcg tgataaacac cactaactcg cgtcgttcat
    33781 cttcttttcc gtcatgacga aataattgcc cgaaccaggg aatgtcgcca agcaacggta
    33841 cgctatcctg acccgattta tttttacggg taaaaatgcc gcccagcgcc aacgtttctc
    33901 cgcttttgac ctcgacctgc gtttcgatct cctgcttatc aatcgccagc acttcgccat
    33961 cggcctgctg tagcacctgc cccggaacgt tctggctgat gtgtaatttc agccggatgc
    34021 gacctttttg taacaccgtg ggcgtgacct ccatccccag gacggcctct ttaaattcca
    34081 ccgacgtcgc gccactttcc ccgctggaaa cctgatatgg aatttcgctc ccctgtttaa
    34141 tgctggcagg ctgaagatgt gaggccagca gacgcggact ggcgataata tccagctgct
    34201 gtttttgttc gagcgcggaa agctcaagat ccagcaagcg tccgttgatg cgcccaatgt
    34261 taaaaccgac atgcgttgtc gccgtcgcta cggagaggtc gctaccaagc gtggtgactt
    34321 gcccaacacc accagcgtgt tgcgcatcgg ccagcgtcca tttcacgcct aactcacgca
    34381 aacttttttc attaatggtg acaatatgcg ccgacagctc aacctgcccg accggaagat
    34441 ccatttgcgc tacccactgt tcaagcgcgc ttaacgccgt tttgttatct cgtagcaaaa
    34501 ggcgattggt gcgtttatcg acggtcatac tccctttggc actcagtagc ttctcccccg
    34561 ctttcgccag ttctcccgcg tcggcgtatt gcagggttat actgcgattt tccagcggca
    34621 gatttgcctg cgcccgcgcc tgctccgcct cctggcgggc gatattgtca ttctgccagg
    34681 caatagaatg cactgagaga atgttgccct cctggcgcgt tatcagcccg gcgcttttca
    34741 ctacagtttg tagtgcctgc ttccagggaa catctgttag atgtaacgac accgtaccgc
    34801 tgacgtctgg cgacacgacc aggttcaact tctcctgttc agccagcgcc tgcaacacct
    34861 gagccaccgg aacgtcatcc accatcagcg tcactttttg cggctttgcc gcctgtacgc
    34921 cgggtatcag catcaacagt agtgcggcta tccattgctt catttgtatc tccttgccgt
    34981 tgccacaacc attgtggcgg ttcgcagttt gtcccggtac ccagcgttag tacgtctggc
    35041 gtcagctgta aaattgtcca gccgttttcc agcacatcgt tttgctgcac ccgtcgccat
    35101 ttcttttgcc cgtcttttat tacaccgatg atgcgctcgc ctcgccctac catcccctga
    35161 tagcgccact ggctaagttc gctaatccgg catagatctt ccggcggttt aaaagggtca
    35221 cgcataccgg ttaaaaggca caatccaata cctgccaaca accagcgttt aaccctcatg
    35281 cggcgtctcc agttgtagcg tgaacaaaag atcatcacct tccacgctta acgaaaaacg
    35341 gctcacgctg acgttgcgct ctgccagccg tgtaaatgcc gacggcactg cttcccacag
    35401 cgttttcaac gccaactcgc ctccctgcgc ggatggatgc caggaaacca gttgcgcgcc
    35461 ggataactga aaatccagtg gcgaaaaggg cagcgttttt tcctcgctga agggagtggt
    35521 gtctaccagg cgatacagtg cggcccactg ctggtgatga cttgcccgta gtcgaattaa
    35581 tgcgtcgcgc tcttcatggt gtgtcgatga tagaaaaatg agcgtaacta acatcagcaa
    35641 ccagaatgcc cagcaaagct ggcggaggcg gggtgatgtg gcgaaccacc agtcaaagaa
    35701 catgttcatc gctaaccttc cttgttaact gatactcaaa ttgccagcgt ccctgcgcat
    35761 cctgctgcgt ggctccccgc tgattgagat gaaaagacgc atcctggcgg agtgacgttt
    35821 ctagtgcgtt taacgcggta atgcttgttg tcagcccctt gatctccagc gttccctgct
    35881 gccagcttat cgttgtcagc caggcgtgct cgggtaaaag cgccgccagt gcttccagcg
    35941 cagattgcca gtcgcgggta aattgtcgct ggcgctgccg ttgcgaacgc tgttcgcgta
    36001 actgctgttg ctccagcaaa cgtggcttcg ttatctgtaa gctgcgggcg agttgttgtt
    36061 ccgcctgaag caaaactgcg tctatgcgcg cttcggcgct gcctgtcaga cgcagtatta
    36121 gcgttatccc gacggccagc agcagaggcg caacgaacat cagcaaccag aaacgcagaa
    36181 aagcggtccg gcgttgctgt cgccagggca aaaaattaat tggcgggttc atcagtacgc
    36241 cttcccaagc gccagcccca gcgcgatggc aaagtctcca cctggcggcg gtagcggcgg
    36301 ctgacgaaca gaaacggcct cccagggatc aaatccccct tcgccacata tcgcgacgct
    36361 ttcgggatca acggataacg ctgccgccag ctccttcgcg ctagtcatcc ccaccgccag
    36421 tttgcgcccc cagcgacagc gtgtcgccca cagccactgt tcgttatcac gccaggccag
    36481 acattgctga tgagaaggta aaaaaggcag gaattgctgt aatgcactgg catccggggt
    36541 gatcgcgctc acatgaacac gcaacctttc tgccagagta agtagcgttg ccagctcttt
    36601 gctttgcgcg gcagtcacgt tataggcggg gctgagtgag tcttcgctgt aatcgaagcg
    36661 cagggagtcc ggatccatat ccagctcgcg ggccatcgtc cctgacagcc aggccgtttg
    36721 ctcccgctca ccaagggaca tcgacgggcg cggaaatgac cgctgtaatg tgcgactggc
    36781 gggaaacgcc aacataatgt gatgacgctg cggcagttcg cgactccacg gtaacaacgt
    36841 tttagccagc tgctgcgcat caacaatccg cccatctttg ataatgtcgt tctccagcgg
    36901 caaccgccac cagcgttgca aaaagcattc ttttgctccc cgcacgatcg caaccgctac
    36961 cgcttcttgc tgttgtaaat gcaaaccaat ttgccagatc ttaaatgcca ttgtgatgat
    37021 ctccttatca cccgtcactc tgacgggtat atcaatgcgt ctggcttgcc tttatactac
    37081 cgcgcgtttg tttataaact gcccaaatga aactaaatgg gaaatttcca gtgaagttcg
    37141 taaagtattt tttgatcctt gcagtctgtt gcattctgct gggagcaggc tcgatttatg
    37201 gcctataccg ctacatcgag ccacaactgc cggatgtggc gacattaaaa gatgttcgcc
    37261 tgcaaattcc gatgcagatt tacagcgccg atggcgagct gattgctcaa tacggtgaga
    37321 aacgtcgtat tccggttacg ttggatcaaa tcccaccgga gatggtgaaa gcctttatcg
    37381 cgacggaaga cagccgcttc tacgagcatc acggcgttga cccggtgggg atcttccgtg
    37441 cggcaagtgt ggcgctgttc tccggccacg cgtcacaagg ggcgagtacc attacccagc
    37501 agctggcgag aaatttcttc ctcagtccag aacgcacgct gatgcgtaag attaaggaag
    37561 tcttcctcgc gattcgcatt gaacagttgc tgacgaaaga cgagatcctc gagctttatc
    37621 tgaacaagat ttacctcggt taccgcgcct atggtgtcgg tgctgcggca caagtctatt
    37681 tcggtaaaac ggtcgaccaa ctgacgctga acgaaatggc tgtgatcgcc gggctgccga
    37741 aagcgccttc cacctttaat ccgctctact caatggatcg tgccgtcgcg cggcgtaacg
    37801 tcgtactgtc gcggatgctg gatgaagggt atatcaccca acaacagttc gatcagacac
    37861 gcactgaggc gattaacgct aactatcacg cgccggagat tgctttctct gcgccgtacc
    37921 tgagcgaaat ggtgcgccag gagatgtata accgttatgg cgaaagtgcc tatgaagacg
    37981 gttatcgcat ttacaccacc atcacccgca aagtgcagca ggccgcacag caggcggtac
    38041 gtaataacgt gctggactac gacatgcgcc acggctatcg cggcccggca aatgtgctgt
    38101 ggaaagtggg cgagtcggcg tgggataaca acaagattac cgatacgctg aaggcgctgc
    38161 caacctatgg tccgctgctg cctgccgcag tcaccagcgc caatcctcag caagcgacgg
    38221 cgatgctggc ggacgggtcg accgtcgcat tgagtatgga aggcgttcgc tgggcgcgtc
    38281 cttaccgttc ggatactcag caaggaccga cgccgcgtaa agtgaccgat gttctgcaaa
    38341 cgggtcagca aatctgggtt cgtcaggttg gcgatgcatg gtggctggca caagtgccgg
    38401 aagtgaactc ggcgctggtg tcgatcaatc cgcaaaacgg tgccgttatg gcgctggtcg
    38461 gtggctttga tttcaatcag agcaagttta accgcgccac ccaggcactg cgtcaggtgg
    38521 gttccaacat caaaccgttc ctctacaccg cggcgatgga taaaggtctg acgctggcaa
    38581 gtatgttgaa cgatgtgcca atttctcgct gggatgcagg tgccggttct gactggcagc
    38641 cgaagaactc accaccgcag tatgctggtc caattcgctt acgtcagggg ctgggtcagt
    38701 cgaaaaacgt ggtgatggta cgcgcaatgc gggcgatggg cgtcgactac gctgcagaat
    38761 atctgcaacg cttcggcttc ccggcgcaaa acattgtcca caccgaatcg ctggcgctgg
    38821 gttcagcgtc cttcacccca atgcaggtgg cgcgcggcta cgcggtcatg gcgaacggcg
    38881 gcttcctggt ggacccgtgg tttatcagca aaattgaaaa cgatcagggc ggcgtgattt
    38941 tcgaagcgaa accgaaagta gcctgcccgg aatgcgatat tccggtgatt tacggtgata
    39001 cgcagaaatc gaacgtgctg gaaaataacg atgttgaaga tgtcgctatc tcccgcgagc
    39061 agcagaatgt ttctgtacca atgccgcagc tggagcaggc aaatcaggcg ttagtggcga
    39121 agactggcgc gcaggagtac gcaccgcacg tcatcaacac tccgctggca ttcctgatta
    39181 agagtgcttt gaacaccaat atctttggtg agccaggctg gcagggtact ggctggcgtg
    39241 caggtcgtga tttgcagcgt cgcgatatcg gcgggaaaac cggtaccact aacagttcga
    39301 aagatgcgtg gttctcgggt tacggtccgg gcgttgtgac ctcggtctgg attggctttg
    39361 atgatcaccg tcgtaatctc ggtcatacaa cggcttccgg agcgattaaa gatcagatct
    39421 caggttacga aggcggtgcc aagagtgccc agcctgcatg ggacgcttat atgaaagccg
    39481 ttcttgaagg tgtgccggag cagccgctga cgccgcctcc gggtattgtg acggtgaata
    39541 tcgatcgcag caccgggcag ttagctaatg gtggcaacag ccgcgaagag tatttcatcg
    39601 aaggtacgca gccgacacaa caggcggtgc acgaggtggg aacgaccatt atcgataatg
    39661 gtgaggcaca ggaattgttc tgattaaaaa ggcgcttcgg cgccttttca gttggctgac
    39721 aaagtgcatt tgtttatgcc ggatgcgggt gaacgcgtta tccggctaac aaaatcgtga
    39781 aaaatcaata tattgcagga accccataag cctgataagc gttgtgcatc aggcaaactt
    39841 cacgcattta cactcgcccc tgccctttca accattcgcg cacgaggaac agcgcgctga
    39901 cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc catcatatgc gccagcggcc
    39961 agcgcacctg tggtagcggc tctggctcat cgccttccag cgattccggg tagagatctt
    40021 gcgctaccac gatattcatt ttgctggaaa aataagacgg tgccatgctg agcttcttca
    40081 aaaaagtcag atcgttcgca ccaaatccaa cctcttcttt tagctcgcgg ttagcggctt
    40141 cgtagacgct ttcacccgga tcaattaatc ctttcgaaaa acctaattcg taggattcag
    40201 ttcccactgc gtattcgcgg atcaggatca ggtgatcgtc cacaatcggc acaatcatca
    40261 ctgcttcccg gttggttgga cgcattcgtt cataaacacg ccgcacgcca ttgctgaact
    40321 ccagatccac gctctcgacg gtaaacagtc gggaacgggc tacagtttca acattcagaa
    40381 tggtgggttt ttgtaatgat ttgctcatcg tgggatctat gctgtgaaat cagcggttat
    40441 tgtgcgatat cggacacgct ttcggcaatg tgaattgcat gttatttaca tttatgtaac
    40501 ttaataaata atcgtcctca aatcaaatta aaagtcaata ggttgaaata actccaggaa
    40561 tttgctgata ttccgccttc agagggtttg ctatgatcag cggttactgt gatgtgctta
    40621 atgatgctca agttaaactc cacgcttgcc gatagccaac cgcagaatca tgtattgtgt
    40681 ctggtgcgac tgaccacgcc tgacagacta agtaagatgg ggaaagcatg agcaccattg
    40741 tgattttttt agctgctttg ctggcctgct cactacttgc gggatggctg ataaaagtgc
    40801 gatccagacg gcgtcagctg ccctggacca acgccttcgc ggatgcgcaa acgcgtaaac
    40861 tcacacctga agaacgtagc gccgttgaaa attatcttga gagcctgacg caggtattac
    40921 aggtgcctgg cccaacggga gctagcgcgg caccgatctc tctggcgctg aatgccgaaa
    40981 gcaacaacgt catgatgctg acacacgcta tcacgcgtta cggcatctct accgacgatc
    41041 cgaataaatg gcgttactac ctcgattcgg tagaagtcca cctgccccct ttctgggaac
    41101 aatacatcaa cgatgagaat accgttgaac tgatttatac cgattcgctg ccgctggtta
    41161 tttcactcaa cggtcatacg ctgcaggagt acatgcagga aacgcgcggc tatgccttgc
    41221 aacctgttcc gtccacgcag gcgtcgattc gcggggaaga gagtgagcaa atcgagctac
    41281 tcaatattcg caaagaaacg catgaagaat atgcgctgag tcgtccgcgc gggctgcgtg
    41341 aagcgttgct gatcgtcgcc tccttcctga tgttcttttt ctgcctgatt accccggatg
    41401 tatttgttcc gtggctggca ggcggcgcat tactgctgct gggcgcaggt ctgtgggggc
    41461 tattcgcgcc cccggcaaaa tcctccctgc gagaaattca ttgtctgcgc ggtacacccc
    41521 gtcgttgggg attgtttggc gaaaacgatc aggaacagat caacaatatt tcactcggta
    41581 ttatcgacct ggtctatccc gcacactggc agccatacat tgctcaggat ctcggtcaac
    41641 aaaccgatat tgatatctat ctcgaccgcc acgtagtgcg tcaggggcga tatctttcgc
    41701 tgcatgatga agtaaaaaac tttccgttac agcactggct gcgcagtacg attatcgctg
    41761 cgggctcgct gctggtgctg tttatgctgt tattctggat cccgctggat atgccgctga
    41821 aattcactct ctcatggatg aaaggcgcgc agaccattga agccaccagc gtaaaacaac
    41881 tggctgacgc tggcgtgcgg gtaggcgata cattgcgtat tagcggtacg ggaatgtgta
    41941 atattcgcac ttccggaacc tggagcgcga aaaccaattc acctttttta ccgtttgact
    42001 gctcgcagat catctggaat gacgcccgct cattaccatt accagaatct gaactggtca
    42061 acaaagcgac ggcattgact gaagcagtta atcgccagct gcaccctaaa ccggaagatg
    42121 aatctcgcgt cagtgcctca ttacgttcag cgattcaaaa atccggcatg gtattgcttg
    42181 atgattttgg cgacattgta ctgaagacag cggatttatg ttctgccaaa gatgactgtg
    42241 tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa agactgggac gcgctggtaa
    42301 aacgcgccaa cgccgggaag ctcgatggcg tgaatgtgtt attacgcccg gtgagtgcgg
    42361 aatcgctgga taacctggtg gcaacctcca ccgcgccgtt tatcacgcat gaaacggcgc
    42421 gagcggcaca atcactaaac agtccggccc ccggcggatt cctgattgtc agcgacgaag
    42481 gcagcgattt tgttgatcag ccctggcctt cggcatcact ttacgactac ccgccgcaag
    42541 aacagtggaa cgctttccag aaactggcac aaatgctgat gcatacgccg tttaacgccg
    42601 aaggtatcgt cacaaaaatc ttcactgacg ccaatggtac gcagcatatt ggccttcatc
    42661 cgatcccgga tcgttccggc ctgtggcgct atctcagcac cacattgctg ctactgacga
    42721 tgctgggtag cgccatttac aatggcgtac aggcctggcg tcgttaccag cgtcatcgca
    42781 ctcgcatgat ggagattcag gcctattatg aaagctgcct gaacccgcaa ctgatcaccc
    42841 cttcagaaag ccttatcgaa taacacgttt gcgcggcagg ttatgctacc ctgtcgcgca
    42901 aattgcttca ctctggagat ttccctcatg catatcaaca ttgcctggca ggacgtagat
    42961 accgttctgc tggatatgga cggcacattg ctcgacctcg ccttcgataa ctatttctgg
    43021 caaaagctgg tgcctgaaac atggggcgcg aaaaacgggg ttacgccaca ggaagcgatg
    43081 gaatatatgc gccagcaata tcacgacgta cagcatacgc taaactggta ctgtcttgat
    43141 tactggagtg agcaactggg tctggatatc tgtgcgatga ccaccgagat gggaccgcgt
    43201 gccgtactgc gtgaagatac cattccgttt cttgaggcac tgaaagccag cggtaagcag
    43261 cgaattttgc tcaccaatgc gcatccgcac aacctggcgg taaaacttaa gcataccggt
    43321 ctggacgcac accttgattt attactttcc acccacacat ttggttatcc gaaagaggat
    43381 cagcggttat ggcatgcggt ggccgaagct acgggtctga aagctgaaag aacgctgttt
    43441 attgatgaca gcgaagctat tctcgatgct gccgcacaat ttggaattcg atactgtctc
    43501 ggcgtcacaa accccgattc cgggattgcc gaaaaacagt atgagcgcca tccgtcactg
    43561 aatgactacc gccgtctgat cccctcgcta atgtgaagga gatgccatga aagagaaacc
    43621 tgtagctgag gttcgactgg ataaatggct atgggccgcc cgtttttata aaacccgcgc
    43681 gctggcccgt gaaatgattg aaggcggtaa ggtgcattac aacgggcagc gcagtaagcc
    43741 gagcaaaatc gtcgagctga atgccacgct cactctgcgc cagggaaatg acgaacgcac
    43801 ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc agcgaggcag ccttgctgta
    43861 tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg gcgctggcac gtaaacttaa
    43921 tgccttaacc atgccgcatc cggaccgacg cccggacaaa aaagagcgcc gcgacctgtt
    43981 acgatttaaa cacggcgaca gtgaataact gtcacctgca agagagatga ttatgccgca
    44041 acatgaccaa ttacatcgct atctgtttga aaactttgcc gttcgcggcg aactggtaac
    44101 cgtttcggaa accctgcaac agatccttga gaatcacgat tatccgcagc ccgttaaaaa
    44161 cgtgctggca gaactgctgg ttgcgaccag cctgttaacc gctacgctga aatttgatgg
    44221 tgatatcacc gtacagctgc aaggcgacgg cccgatgagt ctggcggtca tcaacggaaa
    44281 caacaaacag cagatgcgtg gcgtcgcgcg cgtacagggc gaaattccgg aaaatgccga
    44341 cctgaaaacg ctggtcggca atggttacgt ggtgatcacc atcaccccga gcgaaggtga
    44401 acgctatcag ggcgtggttg gtctggaagg tgataccctg gcggcctgcc tggaagatta
    44461 ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt cgcaccggcg acgtagacgg
    44521 caaaccggct gcgggcggta tgttgttgca ggtaatgcct gcacaaaatg cccagcagga
    44581 cgacttcgac catctggcga cgctaaccga aaccatcaaa accgaagaac tgctgacctt
    44641 accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa gaggtgacgg tttacgatcc
    44701 gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt tgcgccgatg cgctgaaaac
    44761 gctgcctgat gaagaagttg atagcatcct ggcggaagat ggcgaaattg acatgcattg
    44821 tgattactgc ggtaaccact atctgttcaa tgcgatggat attgctgaaa tccgcaacaa
    44881 cgcgtctccg gcagatccgc aagttcatta atgagtatgt ccggcagaga gggctctgcc
    44941 ggattttacg actaatacac ctgatgcgct ttgtcactca ttcagattca catccagcct
    45001 ttcaccatcc actacaagat cgcgccacgc tttaggcttc gttcccagtt ttccatcctt
    45061 cgcagctgtg agtaattttt ctttcgttat atcacgcgga agtaaactca tcgtaagctc
    45121 ttgccattct ttcttgtcag gatcaaagcc atagacaatg tatctttcat catcaaaggc
    45181 aaagaggatc cgctctgccc tgccatcact attcagatct tgctcgacca gtacgcaggc
    45241 gtctttttca atacaggtca tcacgttata gcgttctttg attaatgccg accagaatgc
    45301 cgcatccggt ttgccagaac ctggtgcaat taacacattt tcggctaatg atttttccga
    45361 caccaccttc tgaagacttt gctccccatc taacgccatc agcagatccc gcgcgcgttt
    45421 cgggtctttc ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg
    45481 accgctctgt tcgagcatat agagacttac ctggtctggc gtgtttttgc cgctctaata
    45541 acgcgccata tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag
    45601 caccagtatc accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag
    45661 gggattttgc ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagcaa
    45721 caccagcacc gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg
    45781 cagccataac gcccaggcgg cgacgaacac atacagcgga gcaaccagca aagcggtttt
    45841 aatcaggcaa cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat
    45901 agccattaag atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg
    45961 gcgagaaatc gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa
    46021 tgatactaac ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat
    46081 taaacgtgac tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac ctaatgtaag
    46141 atacataaac cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt
    46201 aaacaactca ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac
    46261 cagtaatata agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc
    46321 attgcggata cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag
    46381 atagcagcca aaatcccaaa gcgccttttc agctctccag gggttcatgc catcagtttg
    46441 ccacttcaac cagccgctca tccccagcgt ggcaataaac accagcgcca gccatcccca
    46501 aaggcgcttc tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt
    46561 cgcaggcacg ccataaacaa tccaactgtg attttttccg gcaagccagg cgatcaacag
    46621 gtagcaaacc agcccctgaa gtaatccggt agcaatcatc ccccagcgtg ttgccggtga
    46681 aagttcaacg ttatccatgt tggttatcca gaatcaaaag gtgggttaat tatcgcatcc
    46741 gggcagtagt attttgcttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat
    46801 actttaccga ttgaatttgc atcaatttca ttaaggaatg cgattccact cacaatattc
    46861 ccgccatata aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt
    46921 ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt
    46981 tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact
    47041 attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc
    47101 cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct
    47161 acgacctgct gtatcaggaa gagctcgatc cgagcctaac aggttatgag cgcggggtgt
    47221 taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag
    47281 ataagtatat cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca
    47341 aaggtaagaa cgacaacaaa cctctctctc cggaaacctg gcagcatctg aaaggcctgg
    47401 tgaccaggca gctttccggc aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc
    47461 cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca
    47521 aaaacatgtt tattcgcccg agcgatgaag agctggcagg tttcaaacca gactttatcg
    47581 ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga acagggtctg aactccgaaa
    47641 acttcgtggc gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg
    47701 gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg
    47761 cttctatgca ctgctccgcc aacgttggtg agaaaggcga tgttgcggtg ttcttcggcc
    47821 tttccggcac cggtaaaacc accctttcca ccgacccgaa acgtcgcctg attggcgatg
    47881 acgaacacgg ctgggacgat gacggcgtgt ttaacttcga aggcggctgc tacgcaaaaa
    47941 ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgtgatgcgt
    48001 tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa
    48061 ccgagaacac ccgcgtttct tatccgatct atcacatcga taacatcgtt aagccggttt
    48121 ccaaagcggg tcacgcaacg aaagttatct tcctgaccgc agacgccttt ggtgtgctgc
    48181 cgccggtttc tcgtctgact gccgatcaaa cccagtacca cttcctctct ggcttcaccg
    48241 ccaaactggc cggtactgag cgtggcatca ccgaaccgac accaaccttc tccgcttgct
    48301 tcggcgcggc gttcctgtcg ctgcatccga ctcagtacgc agaagtgctg gtaaaacgta
    48361 tgcaagcggc aggtgcgcag gcgtatctgg ttaacactgg ctggaacggc actggcaaac
    48421 gtatctcgat taaagatacc cgcgctatta tcgacgccat cctcaacggt tcgctggata
    48481 acgcagaaac tttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg
    48541 tagacacgaa gatcctcgat ccgcgtaaca cctacgcttc cccggaacag tggcaggaaa
    48601 aagccgaaac cctggcgaaa ctgtttatcg acaacttcga taaatacact gacaccccag
    48661 cgggtgctgc gctggtagcg gcaggtccga agctgtaatc agctgatatc tcattgaccc
    48721 cttatccaaa cggataaggg gtttttttat atctgattag ctatcaatat gtacacggta
    48781 cacttataat aatcaataga ccgcaggatg cattatgttc acctttattg aactacaagg
    48841 atttagcaaa cgccgccagt tattgttacc tgatgatgag ttccgggctt ttcaggaact
    48901 actcattcaa gatcccactg ccggtgacat tatcgcaggc acaggcagtt ttagaaaaat
    48961 tcgctggaac cgttctggaa tggggaaacg gggtggcatt cggataattt attacaacgt
    49021 aacacgcaat ggccgaattt atctggcgtt gatttatcct aaaaatgaac aggatgattt
    49081 aacagaagaa caaagaaagg cattgaaact attgtcagag aagctgcttt aatgttgaag
    49141 ctccacaaat tccaccatgg tcgaaatgcc aacggagaca ttgaaaacag atgaaagatg
    49201 aattatttgc tgatcttctt gccagcgcag aagaaatggt acgcatcgaa aagggtgaag
    49261 aaacgcctga accagagcac gtccatactt ttagcgagat cgatgtaaaa gcgatccgtg
    49321 aagcaacggg tttacgtcag caagattttg ctgtcgccgt aggggtaagt tatgacctgg
    49381 tcaaaagctg ggaaaccaaa cgccgtcagc ctaccggagc acctcgaaaa ttgctcctgc
    49441 ttttgcaagc gaatcctttc attatcaatc aattgaaaac aatctaaaaa cgggagacac
    49501 cttcgcctcc cgttttattt acccttcttt tgtcgtgccc tgcgcccgcg ttaccggcac
    49561 tggcagccag gcgcgaatgg aaagcccgcc ccgctcgctg gtgccaagct ccagcatccc
    49621 gttatggtta tccacgatac gctgcacaat tgccagccct aatcccgtgc cgctaatggt
    49681 gcgcgcactg tcgccgcgga caaacggctg gaacaggtgc ttacgttgtt ccggcgcaat
    49741 tcccggaccg tcatcttcca cctggaacca ggcgcgattc ggctccgttc cgctgctgac
    49801 tttgatccag ccattgccat aacgggcggc gttgaccacc atattcgcca ccgcgcgttt
    49861 gatcgacagc gggtgcattt tcacttcaat gctgccgggg taaagcgcgg tttcaatttc
    49921 ccgctcatag ccactttcgg cagcaatcac ctcaccgagt actgcattaa gatccgccat
    49981 ttccatcggc atctcctgcc cggtgcgcag gtagtcgata aactgctcaa tgatggcgtt
    50041 gcactcttcg atatctttat tgatcgattc tgccagatag ccatcctgct cgctcatcat
    50101 ctcagtcgcc aggcgaatac gcgtcagcgg cgtgcgcaag tcgtgactta cccccgccat
    50161 cagcagcgtg cggtcatccg ccagttgctt aacaccagcc gccatatggt taaaggcacg
    50221 ggtaacggaa cgcacctccg aagcgccata ttcacgcagc ggcggcggaa taatcccttt
    50281 accaacctgc aaggctgcgt gttcgagatc gaccaacggt cggttctgga tacgaataaa
    50341 cagccacgcc ccgcctatcg ccaatagcat aatcgccagc gtatagcgga acagcggaga
    50401 gaaatcgccc tgatgaattt cggtcagcgg cacgcgtacc cagatattgg gcgacagcca
    50461 ggttttcagc cagacgacag gcgaactttt gttgacctca acgcgcactt ccgtcgggcc
    50521 gcccagttgc tgtgccatct gatggcttaa gaattcatag tgttgcgccc aacgcagacc
    50581 tgcctcttcg gcagcctcgt tggagtagag agagatcccc agctcacggt agatctcccg
    50641 acggaaagcg ggaggcacaa ccaactgcgt gccgtcctcc agttgcagtt tgtcggtcat
    50701 caacatacgc acttcgtacg cgaggacttt attaaactgc tggaggctcg gcaaaatcgc
    50761 gaagttcagc accaccagat aagttgtcac caggctggcg aacagcaagg tgacgatgag
    50821 caataacgta cgggcaaatg aacttcgtgg cgagaagcgc aatcgcctca tgctttagag
    50881 ccgtccggta caaagacgta gcccaggccc caaacggtct gaatgtaacg cggatgcgct
    50941 ggatcttctt ctaccatgcg gcgcagacgc gaaatctgca cgtcgatgga gcgttccatt
    51001 gcggaatatt cacgaccacg ggcaaggttc atcagcttat cgcgggagag cggctcacgc
    51061 ggatggctga ccagtgcctt cagtaccgca aactcaccgc tggtgagcgg catcggctcg
    51121 tcttcgcgga acatttcgcg cgtaccgagg ttaagtttga acttaccgaa agcaattacc
    51181 gcctcttcct gtgacggtgc gcctggcagt tcgttcgcct gacgacgcag caccgcacgg
    51241 atacgggcca gcagttcacg cgggttaaac ggttttggaa tgtaatcgtc agcgccaatc
    51301 tccaggccaa cgatacggtc cacttcttcc cctttcgccg tcaccataat gatcggcatc
    51361 gggttgctct gactacgaag acgtcggcaa atcgacaagc catcttcacc aggtaacatc
    51421 aaatccagta ccataagatg gaaagattca cgagtcagca ggcgatccat ctgttctgca
    51481 ttagcgacgc ttcgaacctg gaagccttgt tcggtgagat aacgttccag cagcgcacgc
    51541 aggcgcatgt cgtcatcgac caccagaatc ttgtagttct cttgcattgt ttgtactccc
    51601 aaaggttcgc aacaatttgt aagcgtgtat tcttaaaaaa gctcacgttc gtcaccagct
    51661 aaatctggta tgaatttcag cctaaattgt tacaaagcat attaaacagc agcttaagta
    51721 tacaatttat tcggcgaaac attattgatt ctgttgatat gatcacgtta tacccaatgt
    51781 gcgcattatc aaacagacaa agggaatcaa cgagatgaaa acgcccctgg ttacccggga
    51841 agggtatgaa aaactcaaac aagagcttaa ttatctctgg cgtgaagaac gcccggaggt
    51901 cacaaaaaag gtgacctggg ccgcaagtct gggcgaccgc agcgaaaatg ctgactatca
    51961 gtataataaa aagcgtctgc gtgaaatcga ccgtcgcgtg cgctatctca ctaaatgcat
    52021 ggaaaatctc aaaatcgtcg attactcccc gcagcaggaa ggcaaagtct tttttggcgc
    52081 gtgggtggag attgaaaacg acgatggcgt gactcaccgt ttccgtattg tcggctacga
    52141 tgaaattttt ggccgtaaag attacatctc tatcgattcc ccgatggccc gcgcattgct
    52201 gaaaaaagaa gtcggcgatc tggcggtggt gaatacccct gccggggaag cgagctggta
    52261 tgttaatgct atcgagtacg tgaaaccgta aggaagagtc ttaacctcct gccgatggct
    52321 ggcatttttg ccagccagtc cgtataacta tcccctgatt tttgatccga aaagatgaac
    52381 tcaaaccatg atgaatgatt cgttctgccg cattattgcg ggtgaaattc aggcgcgccc
    52441 ggaacaggtt gacgctgccg ttcgcctgct tgacgaaggg aataccgtgc cgtttatcgc
    52501 acgttatcgt aaggaaatca ccggcggtct ggatgacacg cagctgcgta atctggaaac
    52561 gcgtctggga tacctgcgcg aactggaaga gagacgtcag gcgatcctca aatccatttc
    52621 cgagcaaggc aaactcactg acgagctggc gaatgccatc aacgccacct taagcaaaac
    52681 cgaactcgaa gacctctacc tgccctacaa acctaaacgc cgcacccgcg ggcaaatcgc
    52741 cattgaagca gggcttgagc cgttggctga cctgctgtgg agcgatccgt cacacacgcc
    52801 agaagtcgcc gctgcacaat atgttgatgc cgataaaggc gtggcagata ccaaagccgc
    52861 gctggacggc gcgcgctata tcctgatgga acggtttgcc gaagatgccg cgctgctggc
    52921 aaaagtgcgt gattatctgt ggaagaacgc gcatttggtt tctacggtgg tgagcggtaa
    52981 agaagaggaa ggggcgaaat tccgcgacta tttcgatcat cacgaaccgc tgtcaacggt
    53041 gccttctcac cgcgcgctgg cgatgttccg tgggcgtaac gaaggcgtac tccaactttc
    53101 gctgaatgcc gatccgcagt tcgatgagcc gccaaaagag agctattgcg agcaaatcat
    53161 tatggatcat ctcggcctgc gcctgaacaa tgccccggcg gatagctggc gcaaaggcgt
    53221 ggtgagctgg acgtggcgca tcaaggtgct gatgcatctg gaaaccgaac tgatgggcac
    53281 cgtgcgcgaa cgcgcggaag atgaagcaat caacgtcttt gcccgtaacc tgcacgatct
    53341 gctgatggcg gccccagccg gactgcgtgc aacgatgggc ctcgatccgg gtctacgtac
    53401 cggggtaaaa gtagcggtgg tcgatgcaac tggcaaactg gtggcgaccg ataccattta
    53461 cccgcacacc ggacaagccg caaaagcagc gatgaccgtt gctgccctgt gtgaaaaaca
    53521 taatgttgaa ctggtggcga tcggtaacgg tacagcttcc cgcgaaactg agcgtttcta
    53581 tctcgacgtg cagaagcagt tcccgaaagt gaccgcgcag aaagtgatcg tcagcgaagc
    53641 tggcgcgtcg gtttactcgg cttccgagct ggcagcgcag gagttcccgg atctcgacgt
    53701 ttcgctgcgt ggtgcggtat ctatcgcccg ccgtttgcag gatccgctgg cggagctggt
    53761 gaaaatcgat ccgaaatcta tcggcgtagg tcagtatcag catgacgtca gccagacgca
    53821 actggcccgc aaactggatg cagtagtaga agactgcgta aacgccgttg gcgtcgatct
    53881 caacaccgct tctgttccgc tattaacccg cgtggcgggc ctgacgcgca tgatggcgca
    53941 aaacatcgtg gcctggcgcg atgagaacgg ccagttcaag aaccgtcagc aactgctgaa
    54001 agtcagccgt ctggggccga aagccttcga gcagtgcgcg ggcttcctgc gcattaacca
    54061 cggtgataac ccgctggacg cctctaccgt tcacccggaa gcctatccgg tggtggaacg
    54121 cattctggca gcaacacagc aggcactgaa agatctgatg ggtaacagca gcgaactgcg
    54181 taacctgaaa gcgtctgact ttactgatga aaaattcggt gtgccgacag taactgacat
    54241 catcaaagag ctggaaaaac cgggtcgcga tccgcgtccg gaatttaaaa ccgctcagtt
    54301 tgccgatggc gtcgagacaa tgaacgacct gcaaccgggt atgatcctcg aaggcgctgt
    54361 caccaacgtc accaactttg gcgcgtttgt cgatattggc gtgcatcaag acggcctggt
    54421 tcacatctct tcattgtcga acaagtttgt ggaagatccg cataccgtgg tgaaagcggg
    54481 cgacattgtg aaggtgaaag tgctggaagt ggatcttcag cgtaaacgta tcgccctgac
    54541 catgcgtctg gacgagcagc ctggcgaaac caacgcccgt cgcggcggcg gtaatgaacg
    54601 cccgcaaaac aaccgcccgg cagccaaacc acgcggtcgt gaagcgcagc ctgccggtaa
    54661 tagcgcgatg atggatgcgc tggcggcggc aatgggcaaa aaacgttaaa cgccctgatt
    54721 gacccctaaa cctgcgcgat aaggtgtcca cgccgcatct ggcaacgaac gccttgtctg
    54781 acataaaagt gccggagaat atctccggca tttttattcc acagccaaac tcacaatata
    54841 ttccggcaac atttatcact tcattaacaa ctgaaacctt aattaaacat tagccagtcc
    54901 tggtaattcg ctattcgaat tatattttcg ctgcgatata accttgagcc acatcaacat
    54961 tgagtcagat tattattcaa accaacattc gcacacattt taagtattgc tgatagaaac
    55021 cattctcatt atcattgtgc tgttgattat ttaatctctc cttcgttggc aaatcatctg
    55081 gtctcatgtc gctgtcaaac gccccatgag gtagttatcc agttaatgag aaacaagtag
    55141 gcacctatgc aatacactcc agatactgcg tggaaaatca ctggcttttc ccgtgaaatc
    55201 agcccggcat atcgccaaaa actgctttct cttggcatgt tacctggctc ctcttttaat
    55261 gtggtgcgcg tcgctccact cggcgacccc attcatatcg aaacccgtcg tgtgagcctg
    55321 gtattacgca aaaaagatct ggccttatta gaagtggaag cggtttcctg ttaatacggt
    55381 gataacaaca atgaaaaaat taaccatagg cttaattggt aatccaaatt ctggcaagac
    55441 aaccttattt aaccagctca ctggcgcacg tcagcgtgta ggtaactggg ctggcgttac
    55501 cgtcgaacgt aaagaagggc aattctccac caccgatcat caggtcacgc tggtggacct
    55561 gcccggcacc tattctctga ccaccatctc atcgcagacc tcgctcgatg agcaaatcgc
    55621 ctgtcactac attttgagtg gcgacgccga cctgctgatt aacgtggtgg atgcgtctaa
    55681 ccttgagcgt aacctgtacc tgacgctaca actgctggaa ctcggcattc cctgcatcgt
    55741 ggcactgaac atgctcgaca ttgccgagaa gcaaaatatt cgtattgaaa ttgatgctct
    55801 gtcggcgcgt cttggctgcc cagtgatccc gctggtttca acccgtggtc gcggtattga
    55861 agcgctcaag ctggcgattg atcgctataa agctaacgag aatgtggaac tggtgcatta
    55921 cgcacagccg ctactcaacg aagcagattc actggcaaaa gtgatgcctt ccgacatccc
    55981 gctgaaacaa cgtcgctggc tgggcctgca aatgctggaa ggcgatatct acagccgcgc
    56041 ctacgccggt gaagcgtcgc agcatctgga tgccgccctc gcccgtctgc gtaatgagat
    56101 ggacgatccg gcgctgcaca ttgccgatgc gcgttaccag tgcattgctg ccatctgtga
    56161 tgtggtaagc aacaccctga cggcagaacc cagccgtttc accactgcgg tagataaaat
    56221 cgtgctcaac cgtttcctcg gtctgccgat tttcctcttt gtgatgtacc tgatgttcct
    56281 gctggctatc aacatcggcg gggcgttaca gccgctgttt gacgtcggct ccgtggcgct
    56341 atttgtgcat ggtattcaat ggattggcta cacgctccac ttcccggact ggctgactat
    56401 cttcctcgcc cagggcctgg gtggcggtat taacaccgtg ctgccactgg tgccgcagat
    56461 tggcatgatg tacctgttcc tctccttcct tgaggactcc gggtatatgg cgcgtgcggc
    56521 gtttgtgatg gaccgtctga tgcaggcgct gggcttgccg gggaaatcct ttgtgccgct
    56581 gatcgtcggt ttcggttgta acgtaccgtc ggtaatgggt gcacgtacgc ttgatgcacc
    56641 gcgtgaacgt ctgatgacca tcatgatggc accgtttatg tcctgcggcg cgcgtctggc
    56701 tatcttcgca gtattcgcgg ctgccttctt cgggcagaac ggtgcgctgg cggtcttctc
    56761 gctgtatatg ctgggtattg tgatggcggt gctgactggc ctgatgctca agtacaccat
    56821 catgcgcggt gaagcgacgc cgtttgtcat ggagttgccg gtctatcatg taccacacgt
    56881 taaaagcctg attatccaga cctggcagcg tctgaaaggc ttcgttctgc gtgctggtaa
    56941 agtgatcatc atcgtcagca ttttcctgag cgctttcaac agcttctcgc tgagcgggaa
    57001 aatcgtcgat aacatcaacg actcggcgct ggcgtccgtc agccgggtga tcaccccggt
    57061 cttcaagcca attggcgtgc atgaagataa ctggcaggca acggttggcc tgtttacagg
    57121 tgccatggcg aaagaagtgg tagtgggtac gctcaacacc ctctacaccg cagaaaatat
    57181 tcaggacgaa gagttcaatc cggcagagtt taacctcggt gaagagctgt tcagtgcgat
    57241 agatgaaacc tggcagagcc tgaaagacac cttcagcctt agcgtactga tgaaccccat
    57301 tgaagccagc aaaggcgacg gcgaaatggg taccggggcg atgggcgtga tggatcagaa
    57361 attcggtagc gcagcagcag cttacagcta cctgattttc gtcctgctgt atgtaccatg
    57421 tatctcggtg atgggagcca tcgcccgtga atcaagccgt ggctggatgg gcttctccat
    57481 cctgtggggg ctgaatatcg cttactcgct ggcaacattg ttctatcagg tcgccagcta
    57541 cagtcagcac ccaacttaca gcctggtgtg cattctggcg gttatcctgt ttaacatcgt
    57601 ggttatcggt ctgctgcgcc gcgcgcgtag ccgggtggat atcgaactgc tggcaacccg
    57661 caagtcggta agcagttgct gcgcagccag caccaccggt gattgccatt aatggcttca
    57721 cttattcagg tgcgcgattt gctggcgtta cggggccgta tggaagcggc ccagataagc
    57781 cagacattga acactccaca gccaatgatt aacgccatgc tgcaacaact ggaaagtatg
    57841 ggcaaagccg tgcggattca ggaagaacct gacggctgcc tctctggcag ttgtaaaagc
    57901 tgcccggaag gaaaagcctg tctgcgcgag tggtgggcgc tgcgttaacc ttactccatc
    57961 gcctgttttg gaaagcccgg tatgcgtctg catccgggct tttttgcgtg cggctttcca
    58021 taaaaatgca actcttgcag cacggcgtaa gttcctttga aagcatctcg cagggatgaa
    58081 aactcgctaa tacacaggtg tggagtggcg cgtagagtcg cggcattcaa acaacaggtg
    58141 aaggaacgcc atgagcaaaa agcagagttc caccccacac gatgcgctgt tcaaactctt
    58201 tttacgccaa ccggacacgg ctcgtgattt tcttgcgttt catttaccgg cacccattca
    58261 cgcgctttgt gatatgaaaa ccctcaagct ggagtcgagc agctttattg atgacgatct
    58321 gcgtgaaagc tattccgatg tgctgtggtc ggtgaaaacg gaacaaggac caggatacat
    58381 ctattgtctg attgaacatc aaagcacctc aaacaaactg atcgcatttc gcatgatgcg
    58441 ttaagctatt gccgcaatgc aaaatcacct tgatgctgga tacaaaacgt tgccgatggt
    58501 ggtgctattg ttgttttacc acggtattga aagcccctat ccctattcgc tgtgttggct
    58561 ggattgtttc gccgatccca aactggcaag gcagctttat gcctccgcat ttccgctgat
    58621 tgatgtcacc gtcatgcctg atgatgaaat catgcagcac cgacgtatgg cgctgctgga
    58681 gttaattcaa aaacatattc gtcaacgcga cctgatgggg ctggtagagc aaatggcctg
    58741 cttattaagt agtggatacg ctaatgacag acaaatcaaa gggctgttta attacatact
    58801 gcaaaccggc gacgcggtac gttttaacga ttttatcgac ggcgttgccg aacgttcacc
    58861 gaaacacaag gagagtttaa tgacaattgc ggaaagattg cggcaggagg gggaacaatc
    58921 caaagccctg catatagcca aaataatgct tgaatccgga gtccctctta cagacatcat
    58981 gcgctttacc ggggtgtcag aagaagagtt ggctgcggcg agccagtaaa tttctgtctc
    59041 gccatttcaa aagttgccta caccctctgc ttcaacgcca ccagcaggtg acaaaactcg
    59101 accggatgcg aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga
    59161 ggccaaagtt tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca
    59221 tacaatcgca aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc
    59281 aggatttcca gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt
    59341 ttcttcaacg cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg
    59401 aaccgctcca ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg
    59461 tccggtttta tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg
    59521 accagcgcct gaacacgctc gggatgggtt aaaacaattt ggcttgccac cagcccgccc
    59581 agactccagc ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata
    59641 tcagcaagtg acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg
    59701 tgcagcgtaa aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt
    59761 ccccatccgt gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg
    59821 ttattcatcc gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg
    59881 ggattatgct ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc
    59941 tgttcacgcg ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca
    60001 cactcccatc ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc
    60061 acggttgccg actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg
    60121 cgcagtgaaa tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt
    60181 cgcaccaccg ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt
    60241 cactggcgtc ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg
    60301 cattgccaat gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt
    60361 ctcagtgccc ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg
    60421 caaggtcgcc atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag
    60481 attgcgcagt tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga
    60541 accttgtaga gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc
    60601 cattactatg atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa
    60661 tcaggaagaa gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga
    60721 atgtggtgtt tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt
    60781 tgacctgctg accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat
    60841 cgactttgtt accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat
    60901 gcgtaaagtg gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca
    60961 gatcaaccca cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga
    61021 cggttacgcc attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac
    61081 gctgaaagaa gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg
    61141 cgatctcacc gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt
    61201 agattgcccg atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg
    61261 ttggcgcagc attgctttgt caaatcctcg gttcgtttct ccgcgataat atgaccaacc
    61321 tctcataatt taaatttacc ccgctctggt gattctcaaa cgccagatgt tacccgtatc
    61381 attcacatgg gtaccaaaca tactcctgac atctgactac aataattagt tttagtgggt
    61441 atcagtcgtg gtgccgcaat atctctgttc ccgattggga taattagagt ttgtcgtcag
    61501 aaaattgacg ttacccataa caaatgaaag gccaggtaaa tcatgccatt agtcattgtt
    61561 gctatcggtg taatcttgtt gttgctcctg atgatccgct tcaaaatgaa cggcttcatc
    61621 gctctcgtcc tcgtggcgct tgctgttgga ttaatgcaag gaatgccgct ggataaagtt
    61681 attggctcca tcaaagccgg tgtcggcggg acgctcggta gccttgccct gatcatgggt
    61741 tttggcgcaa tgctgggcaa aatgctggca gactgcggtg gcgcacaacg tatcgccacc
    61801 acgctgattg ccaaatttgg taaaaagcac atccagtggg cggtggtgtt aaccggtttt
    61861 accgttggtt ttgccctgtt ctatgaagtg ggcttcgtgc tgatgctgcc tctggtgttt
    61921 accatcgcgg cttccgcgaa tattccactg ctttatgttg gtgtaccaat ggcggctgca
    61981 ctgtctgtga cccacggctt cctgccaccg catccgggcc cgactgcgat tgccaccatt
    62041 ttcaatgccg atatgggtaa aaccctgctg tacggtacta ttctggcaat cccgaccgtg
    62101 attctcgccg gtccggttta cgctcgcgtg ttgaaaggta tcgataagcc aatcccggaa
    62161 ggtctctaca gcgcgaaaac cttcagcgaa gaagagatgc cgagctttgg cgtcagcgtc
    62221 tggacctctc tggtgccggt agtgctgatg gcgatgcgtg cgattgccga aatgatcctg
    62281 ccgaaaggtc acgctttcct gccggtagcg gagttcctcg gtgacccggt aatggctacg
    62341 ctgattgccg tgctgattgc gatgttcacc tttggtctga accgtggtcg ttcaatggat
    62401 cagattaacg acacgctggt ttcttcgatc aaaatcattg cgatgatgct gttgatcatc
    62461 ggtggtggcg gtgcgttcaa gcaggtgctg gtagacagcg gcgtggacaa atacattgct
    62521 tccatgatgc acgaaaccaa catttctccg ctgctgatgg cctggtcgat tgctgccgta
    62581 ctgcgtatcg cgctgggttc cgcaaccgtt gcagcaatca ctgcgggtgg tatcgcggca
    62641 ccgctgattg caacgacggg tgttagccca gagctgatgg ttattgcggt gggttccggt
    62701 agtgtgattt tctctcatgt gaacgatccg ggcttctggc tgttcaaaga gtactttaac
    62761 ctgactatcg gcgagaccat caaatcctgg tcgatgctgg aaaccattat ctcggtgtgc
    62821 ggtctggtag gctgtctgct gctgaatatg gtgatttgat aacactgact gccggatgca
    62881 tcgcatccgg catcaaccgc actctacttc ttcttcgctg cagctctgcg ccgtctgtcc
    62941 aaatccttca gcaacttgtt cacgccatca tcggcaaaca tcgactcaag cgttgcggaa
    63001 agcttgcgtc gccagttttt atactggtaa ctggtgccag gaatattcac cggttcggcc
    63061 atatccagcc agtcttccgg ctgtagtcct aacagagcac tgttactgtc ggcaatgtag
    63121 cgctgcaaac cacggttcag cgtcggcgtc atcgacatca acgatgcctt atgcccggca
    63181 cgttttggca gacaaccata tttatgcagt gcatccagca gcccttgctt cgccagttcg
    63241 cgatcctgat acagaccgcg cagtaccact tcatccggat acagccccag ggttttgccc
    63301 aacgttagat ccccgctctc ccagtaaccg cgcagcgtag gcaggtcatg tgtcgccgca
    63361 accgccatcg actgctccgg atacgctttc ggtgcacgga acgtcttctc gtggtcgttt
    63421 tcgaaataga gcactttgta ggagtacaca ccgctgctgc gcagcttacc gacaatctct
    63481 accggtacgg taccgagatc ttcaccaatc accatacagc gatgacgttt actttcgagt
    63541 gccagaatcg agagcagatc atccaccgga tagtgaacat acgcgccctg atctgccgtc
    63601 tcgccatacg gtatccacca caaacgcagc atcgacatca catggtcaat tcgtaatgcg
    63661 ccgcagtttt gcatattggc acgcaacagc tcgataaacg gttcataggc acgcgcggtg
    63721 atgatatgcg ggtccattgg cggtaatccc cagttctgcc ccaacgggcc gaggatatcc
    63781 ggcggcgcgc caaccgacgc tttcaggcaa tatagttcac ggtcacacca ggtttccgcc
    63841 ccaccttccg ctacgccaac cgccagatca cgatacaagc caatcggcat ttcatagccc
    63901 tggcttatct cccagcaggc ggcaaactgg ctgtaagcca gccactgcaa ccagagataa
    63961 aaatcgacgt cattacgatg ttcttcgcag aactgacgca cttctggtga atccacgttc
    64021 tgatacatct ctggccatgc aggccagccc cagcgcattt cgtcctcttt cacttgctgg
    64081 gcatgtagcg catcaaaggc tgcctgccag aacaggctgt cgccctgctc tgcaacaaac
    64141 tggcgaaacg cggccatctg ctcatcatca cgttgcgcga aacctttcca cgccattcgt
    64201 aatgctgtca tttttagggc ggtaaccgtg gagtaatcga cccagtcggc atcgcgcgcc
    64261 tgttgcagcg tctgttgcgt ggtcggcaac tgccaccagg cctgagcctc ttcgctaaga
    64321 tggaaatctt caacggcgtt aacgtcgata taaatcacat tcagccaacg gcgagaagac
    64381 gggctgtatg ggctggcact ctccggattt gccggataga gcgcatgaat cgggttcagg
    64441 ccgatgaacg acccgccacg tattgccaca tccaccagca tcgctttgag atcgccaaaa
    64501 tccccaatac cccagttttt ttccgatcgc agcgtataaa gctgaacgca ggcaccccac
    64561 agcttttgtt tattcagcaa cgcctgcggt tcgtaacagc gtttcggggc gacaatcacc
    64621 cggcaatgcg cgcgctggtc gtcctgggtg agtgtcagcg tgtgataacc ttccggcagc
    64681 ttcgtcggta gattgaacgc tttgcccccc gttacatggc ctttgtactg tgttccttct
    64741 tcggtggtca gcagccagct atattcgccg ctgccctcca ccaccatcgg catttttttg
    64801 ccgctggtat aaaccatgac attcgggact ggcgttaccg ccactttcgt ggcggtacgt
    64861 tgatgcatcg cgtcaagcaa acgccgtttg gtttcggcgc taatcgactg cggtttaccg
    64921 tgggcattga tgtaattggg gctaatcccc gccgccagcg cggcattatc cagacgtttg
    64981 ctttccatcg agcttcctta gcgttttgcc tgccagatac gagcctgata atcgcgaata
    65041 gagcgatccg agctaaacat accgcagcgg gcggtattga ggatcgccgc gcgagtccag
    65101 gcctcctggt cgcggtacag cacatccacc tgcttttgtg cctcaacata ggctgcgaaa
    65161 tccgccatca ccagatacgg atcgccgccc tgtttgccga tactgtgcag catctggtcg
    65221 aaggcatgct tatcgccgtc gctgtattta ccgctttcca gctctttcaa tactgcgtcc
    65281 agcaccttat ctttcttccg ccatttcacc gggtcgtagc ctttggccag aatagccttc
    65341 acttgttcca cggtatgacc aaaaataaag atattttctt caccgacttt ctcggcgatt
    65401 tcaacgttcg caccatccag cgtaccgaca gtaagcgcac cattgagcgc cagtttcata
    65461 ttgccggtac cggaagcttc tttacctgca gtcgaaattt gttcggagat atccgccgcc
    65521 gggatcagtt tttccgccgc cgaaacgcaa taatccggca ggaacaccac cttcaactta
    65581 tcgccaacca gtggatcgtt gttgatcacg tcagccactt tgttgatcgc aaagataata
    65641 ttcttcgcca ggtagtagcc cggtgccgct ttcgcgccga agaggaagac gcgcggtacg
    65701 cgatcagcct gcgggttttc acgaatttct ttgtacaacg ccagaatatg cagcagattc
    65761 aggtgctggc gtttgtactc atgcagacgt ttgatctgaa tatcgaaaat cgcctgtgga
    65821 ttgatctcaa taccggtacg aactttcaca aactccgcca gacggacttt attcgcctgc
    65881 ttgatctcgc gatattgctg acggaatttc gcatcatcag cgaatttttc cagattgatc
    65941 agctgatcga gatcgttagc ccactctttt tgcagtgatt tatccaacag agccgccagt
    66001 gcagggttgc actgtttgat ccagcgacgt ggggtaatac cgttggtgac gttatggaat
    66061 ttgttcggcc atagctggtg atattccggg aacagatctt tcaccaccag atccgagtgc
    66121 agcgccgcaa caccgttcac cgcgaaaccg ccaaccacac acaggttcgc catatgcact
    66181 tgtttatcgt gcaccaccgc cagtttggcc cacacttttt catcgcccgg ccaggttttc
    66241 tccaccagcg ttttaaagcg agtattaatt tcgttaataa tctgcatatg gcgcggcagt
    66301 aagcctttca ccagtttcac atcccagcgt tccagcgctt ctggcatcag ggtatggttg
    66361 gtgtaagcga aagttttgct ggtaatggcc caggcgtcat cccagctcat ctggtgctca
    66421 tcgatcagca cgcgcagcag ttctggaatc gcgatagttg ggtgggtatc gttcagctga
    66481 ataacttcgt aatccgccag ttcgtgcagt ttacgccccg ccagatgatg gcgacgcaaa
    66541 atatccgcta ccgaacaggc acactggaag tactgctgca tcaagcgcag ctttttaccg
    66601 gcagtatggt tgtcgtttgg atagagaact ttggtcagtt tttccgcatt gatgccctgc
    66661 tgttcggcac gcaggaaatc accgtcgtta aatttagtca gatcaaacgg atgcgcgtgc
    66721 gtcgcctgcc acagacgcag cggctgcgcc acgccattac gatagccgac aacggggaga
    66781 tcccacgctt gaccggtaat ggtaaactcc ggctcccagc gtccgtcttt cgtcacttta
    66841 ccgccaatcc ctacctgcac atccagtgct tcgttgtggc ggaaccacgg gtagttactg
    66901 cggtgccagt catccggcgc ttcaacctgt ttgccatcga caaaagactg gcggaacaaa
    66961 ccatattgat agttcagacc gtaacccgtc gcagactgac cgacagttgc cattgagtcg
    67021 aggaagcacg ccgccagacg tcccagacca ccgttaccca gcgccgggtc gatctcttct
    67081 tccagcaggt ccgtcagatt gatgtcataa gccttcaacg aatcctgtac atcctgatac
    67141 cagccgagat tcaacaggtt gttgcccgtc aggcgaccaa tcaaaaactc cattgagatg
    67201 tagttaacat gtcgctgatt cgccaccggc ttggcgaatg gctgagcacg cagcatttcg
    67261 gccagtgctt cactcactgc tagccaccac tggcgaggag tcatttcagc cgcagaattt
    67321 aagccataac gctgccactg acgtgaaagc gcttcctgaa attgcttatc gttaaaaata
    67381 ggttgtgaca taggagttcc acttttctta gattttcaac acaacgttat cgctagtttg
    67441 ccaggctcga tgttgacctt cctcatcctg cgggggatta ggcagggagg agttgcgggg
    67501 atgagcaagg aaatgtgatc tcaaccactt aaagctagtg caaaccacag gattagcagc
    67561 aaatcaatgc aatacagcgc agaaaatctg tatctaagtg caaaaaatgg ccgttgcgta
    67621 ttttcaaaaa gcggaaggta actctataaa ttaagtaaag gagtgaaaca gtttcataag
    67681 taaaatatcc agtgtgctcc atctcattct taatagattt attaagatca tctttttaga
    67741 tggcactttc atcaggaatg aagaagaaac ccttgcttaa atgaatctga tgaacataag
    67801 ggaaaccagt attcacgctg gatcagcgtc gttttaggtg agttgttaat aaagatttgg
    67861 aattgtgaca cagtgcaaat tcagacacat aaaaaaacgt catcgcttgc attagaaagg
    67921 tttctggccg accttataac cattaattac gaagcgcaaa aaaaataata tttcctcatt
    67981 ttccacagtg aagtgattaa ctatgctgat tccgtcaaaa ctaagtcgtc cggttcgact
    68041 cgaccatacc gtggttcgtg agcgcctgct ggctaaactt tccggcgcga acaacttccg
    68101 gctggcgctg atcacgagtc ctgcgggcta cggaaagacc accctcattt cccagtgggc
    68161 ggcaggcaaa aacgatatcg gctggtactc gctggatgaa ggtgataacc agcaagagcg
    68221 tttcgccagc tatctcattg ccgccgtgca gcaggcaacc aacggtcact gtgcgatatg
    68281 tgagacgatg gcgcaaaaac ggcaatatgc cagcctgacg tcactcttcg cccagctttt
    68341 cattgagctg gcggaatggc atagcccact ttatctggtc atcgatgact atcatctgat
    68401 cactaatcca gtgatccacg agtcaatgcg cttctttatt cgccatcaac cagaaaatct
    68461 caccctggtg gtgttgtcac gcaaccttcc gcaactgggc attgccaatc tgcgtgttcg
    68521 tgatcaactg ctggaaattg gtagccaaca actggctttt aatcaccagg aagccaagca
    68581 gttttttgac cgtcgtctga catcacctat cgaagctgcg gaaagcagcc gtatgtgtga
    68641 cgacgtctct ggctgggcca cggcactgca acttatcgcc ctttctgccc gacaaaacag
    68701 ccattcggcg catcagtcag caaggcgcct ctcaggtatt aatgccagcc atctttcgga
    68761 ttatctggtg gatgaggtgt taaacaacgt cgatctcgca acgcgccatt ttctgttgaa
    68821 aagcgccatt ttgcgctcaa tgaacgatgc cctcatcacc cgtgtgaccg gcgaagaaaa
    68881 cgggcaaatg cgtctcgaag agattgagcg tcaggggctg tttttacagc ggatggatga
    68941 taccggcgag tggttctgct atcacccgct gtttggcaac ttcctgcgcc agcgctgcca
    69001 gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc gccgcagaaa gctggatggc
    69061 ccagggattt cccagcgaag cgattcatca tgcgctggcg gcaggcgatg cgctgatgct
    69121 gcgcgatatt ctgcttaatc acgcctggag tttgttcaac catagcgaac tgtcgctgct
    69181 ggaagagtcg cttaaggccc tgccgtggga cagtttgctg gaaaatccgc agttagtgtt
    69241 attgcaggcg tggctaatgc aaagccaaca tcgctatggc gaagttaaca ccctgctagc
    69301 ccgtgctgaa catgaaatca aggacatcag agaaggcacc atgcacgcag aatttaacgc
    69361 tctgcgcgcc caggtggcga ttaacgatgg taatccggat gaagcggaac ggctggcaaa
    69421 actggcactg gaagagctgc cgccgggctg gttctatagc cgcattgtgg caacctcggt
    69481 gctgggtgaa gtgctgcact gcaaaggcga attgacccgc tcactggcgc taatgcagca
    69541 aaccgaacag atggcacgcc agcacgacgt ctggcactac gccttatgga gtttaatcca
    69601 gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc gcgtgggaaa cacaggaaaa
    69661 agcgttccag ctgatcaacg agcagcatct ggaacagctg ccgatgcatg agtttctggt
    69721 gcgcatccga gcacaactgt tatgggcctg ggcacggctg gatgaagccg aagcgtcggc
    69781 gcgtagcggg attgaagtct tgtcgtctta tcagccacag caacagcttc aatgcctggc
    69841 aatgttgatt caatgctcgc tggcccgtgg tgatttagat aacgcccgta gccagctgaa
    69901 ccgtctggaa aacctgctgg ggaatggcaa atatcacagc gactggatct ctaacgccaa
    69961 caaagtccgg gtgatttact ggcaaatgac cggcgataaa gccgccgctg ccaactggtt
    70021 gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc ctgcaaggtc aatggcgcaa
    70081 cattgcccgc gcacaaatct tgctgggcga gtttgaacct gcggaaattg ttctcgaaga
    70141 actcaatgaa aatgcccgca gtctgcggtt gatgagcgat ctcaaccgta acctgttgct
    70201 gcttaatcaa ctgtactggc aggccggacg taaaagtgac gcccagcgcg tgttgctgga
    70261 cgcattaaaa ctggcgaatc gcaccggatt tatcagccat tttgtcatcg aaggcgaagc
    70321 gatggcgcaa caactgcgcc agctgattca actcaatacg ctgccggaac tggaacagca
    70381 tcgcgcacag cgtattctgc gagaaatcaa tcaacatcat cggcataaat tcgcccattt
    70441 cgatgagaat ttcgttgaac gtctgctaaa tcatccggaa gtgccagaac tgatccgcac
    70501 cagcccgttg acccaacgtg aatggcaggt cctggggctg atttactccg gttacagcaa
    70561 tgaacaaatt gcgggcgaac tggaagtggc ggcaaccacc atcaagacgc atatccgcaa
    70621 tctgtaccaa aaactcggcg tcgcccaccg tcaggctgcc gtgcagcacg cgcagaaact
    70681 gctgaagatg atgggttatg gggtgtgagt ttagccggat aatgcattgg atccggttta
    70741 catctttaca tcattcaata ctcacccgcg ttacgccatc tgtttctatc aaaccaaacc
    70801 gcaccggcaa gaaacgctcc accaccgcga tattggtcag cagatggcat gaaggatggg
    70861 cgaccttaaa ttctcccgcg cccgccagcg ccatcggcag taccagttgg tcggcgagat
    70921 attcccccac cgccgctggg cttgccaggt agcgtttcac ctctttcact aactgcgcgg
    70981 ctaccacctc ggcactgacg cgcttttcac cgacgacgaa aaagcgttca gtgatatttt
    71041 cactttcgac ttccagcgag acggtattac ccggcccctg gtcgcgcggc aggttatgaa
    71101 tattctgttc atgcagggaa aaactccccg ccagtgtagc gatttcacgc tcagcaacat
    71161 ggcgcggcac accagctaat agaacttctc cacgcatctg cacaatgttc ccgcgctcgc
    71221 caagttgcaa ggtgttaaac gatgccacgg gcgagacttc cgttgccacc acgccgcctc
    71281 cggcaggata aaaaccgtgg cgtaacagcg tggtttgctg atgaattcct atttttgcca
    71341 gcagcggctc cagcacccgg cggataaaat ccgcaggtgg cgccgacggg ttatcggtac
    71401 cgccgctcac ttcaacacgc gaaggtccat cggcaaacca cagcgcgggc agcacagttt
    71461 gcagcaccag cgtacaactt ccggcgctac cgatagcaaa gcggtaatcg ccgccgcgca
    71521 ctgtaccggg ccggaagacc agacgctgcg accccagctc cgcgccttcc accgttgctc
    71581 ggcaaatttc cgtcgccgcc ttaaccgcgg tcagatgctg gcgcaacagt cccggtttcg
    71641 cccgcccggc acgaatgccg gtgatggtaa atggctggcc ggttatcatc gacagactca
    71701 gtgccgagcg caggatctgc ccgccgcctt cgccctgtgc gccatccagc gcaatcatcc
    71761 ttttcatcat ttatccttta acgcacacca cctgacgcag ggtatagata acttccacca
    71821 gatcgctttg tgccgccatc accgcatcaa tatctttata cgccatcggg atttcgtcga
    71881 tcacttcggc atctttacgg cattccacat gcgcggtggc acgaatttga tcttccacgc
    71941 tgaacagttt tttcgcttta gtacggctca taacccgccc ggcaccgtgg ctgcacgaac
    72001 agaacgactc ttcatttccc agcccacgga cgataaagct ttttgctccc atcgaccctg
    72061 gaataattcc atattgacca gcacgcgcag acaccgcgcc tttacgcgtc acgtagatct
    72121 cttcaccaaa gtgctgttct ttttgcacat agttgtggtg acagttgatc tcttccatcg
    72181 ccagcgtttg tggctgtctg accgttttct gcgtaatgct ctgcaacgcc gttaccacgt
    72241 tttccatcat cgcatcgcgg ttaaggctgg cgaaaagctg cgcccaggcc acggctctta
    72301 ggtaatcatc aaagtattcc gtaccttcca taaagtacgc cagatcacgc gacggcaacg
    72361 tctcaagcgt ttcctgcatc tctttttgcg ccagatcgat aaagtaagtc ccgatggcgt
    72421 taccaattcc gcgtgaaccg gagtgcagca taatccacac ctggtccgcc tcatcaaggc
    72481 agatttcaat aaagtggtta cccgttccca gcgttcccag gtgtttatag ttgttagtgt
    72541 taaggaaacg tggatatttt tgcgttaacc actgataacc ggcttccagc tcagcccatt
    72601 tagcatcgac gttaacaggt ggattttccc aggcaccttt atcacgttta caacggccag
    72661 tggtacgccc gtgcggcacg gccgtttcaa tcgcctgacg cagctctgcc aggttttcag
    72721 gcaggtcttc cgccgttaac gcggtacgca gcgcgttcat tccacagcca atatccacgc
    72781 ccaccgccgc cggaataatc gcccctttgg tcgggatcac gctaccaatg gtggaacctt
    72841 tacccaggtg tacatcaggc attaccgcaa tatgtttgaa aataaacggc atcttcgccg
    72901 tattaataag ttgctgacgc gcatcggcct ctaccggcac gcctttggtc cacattttta
    72961 ccggggcatt ttcagtggtc agtaattcgt aattcatttt gtttttctct tttcgttgtt
    73021 tgctgtcctg ataattgcaa ccgtcgtgcc agaaaattaa ataagcagct taatttttta
    73081 attcattgtt ttaaaaaaga ttatatcttt acgtccgtaa ccggagattt cccgcaaagc
    73141 caatttaccg ataatgaaat atcgtctttt ataaggatat ctaagatgcg taaaacagtg
    73201 gcttttggct ttgtcggtac cgtactggat tatgccgggc gcggcagtca gcgctggtca
    73261 aaatggcgtc cgacactctg tttatgccag caagaatcgt tggtcatcga tcgactggaa
    73321 ttgttgcacg acgcccgctc gcgctcgcta tttgaaacgc ttaaacgcga tatcgccagc
    73381 gtttcgccag aaacagaagt ggtgagcgtt gagattgaac tgcataaccc gtgggatttc
    73441 gaagaggtct acgcctgcct gcatgatttc gcccgtggtt acgagtttca gccagaaaaa
    73501 gaagactatt taattcacat caccaccggt acccacgtcg cgcagatttg ctggtttctg
    73561 ctggcagaag cgcgttacct gcccgcccgg ctgatacaat cttcacctcc gcgcaaaaaa
    73621 gaacagccgc gcggcgcagg tgaagtgacg attatcgatc tcgatttaag ccgttataac
    73681 gccatcgcca gccgctttgc cgaggaacgc cagcaaacgc ttgattttct taagtccggc
    73741 attgccacgc gtaaccccca cttcaaccgc atgattgagc agatcgaaaa agtggcgatc
    73801 aaatcccgcg cgccgattct gcttaacggt ccaaccggcg cgggcaagtc atttctggcg
    73861 cgacgcatct tcgagttaaa acaggcgcgg catcagttta gcggcgcgtt tgtggaagtg
    73921 aactgcgcca ccctgcgcgg cgataccgcc atgtcgacgc tgtttggtca tgtaaaaggc
    73981 gcgtttaccg gggcgcggga atctcgtgaa ggtttattac gcagcgccaa cggcggaatg
    74041 ttgtttcttg atgagattgg cgaactgggc gcagacgaac aagcaatgct gctaaaagcc
    74101 attgaagaga aaacctttta cccgtttggc agcgatcgcc aggtgagcag tgattttcag
    74161 cttatcgccg gaacggtgcg cgatttgcgc cagctggttg ccgaaggcaa atttcgcgaa
    74221 gatctgtacg cgcggatcaa tctctggacc ttcaccctgc cgggtctacg ccagcgccag
    74281 gaagatattg aaccgaacct tgattatgaa gtggagcgcc acgccacact cactggcgac
    74341 agcgtgcgtt ttaacaccga agcgcggcgc gcctggttgg ctttcgcgac ctctccccag
    74401 gcaacatggc gcggtaactt tcgcgaactt tctgccagcg tcacgcggat ggcaaccttt
    74461 gccactagcg gacgcatcac tctggacgtg gttgaagatg agataaaccg tctgcgctat
    74521 aactggcagg agagccgccc ctccgcgctt acggcgttgc tgggcgcaga ggcagaaaac
    74581 atcgatctct tcgaccgtat gcaactggaa cacgttatcg ctatctgccg ccaggcaaag
    74641 tcgctttccg cagccggacg ccagcttttt gacgtttcgc gccagggcaa agccagcgtc
    74701 aatgacgcgg atcggctacg caaatacctg gcgcgttttg gtctgacgtg ggaagccgtg
    74761 caggatcagc acagctccag ttgaatatgg tggtccgtca gcacctgcat cacgcttact
    74821 ggcggcgggg cgtcggtgta gacggcatct accatgctga tgctgcccat attgaccatc
    74881 gcgttacggc caaatttcga gtgatcgaca accagcataa cgtggcgcga gttctcaata
    74941 atggcgcgct tggtgcgaac ttcgtggtaa tcgaactcca gcagcgagcc gtcactgtca
    75001 atgccgctga tccccagaat gccgaaatca aggcggaact gggagataaa atcgagtgtc
    75061 gcttcgccaa tgatcccgcc atcgcggctg cgtaattcgc caccggcgag aataatacgg
    75121 aaatcctctt ttaccatcag cgtgttagca acgttaagat tgttggtgac aatacgcaaa
    75181 ttgctgtgat taagcagcgc gtgcgccacc gcttccggcg tggtgccgat atcgataaac
    75241 agtgtcgagc cgttggggat ttgctccgcc actttgcggg caatcctctc tttttcttcg
    75301 gtctgggtgg ctttgcgatc gtgccacggc gtgttaaccg aactggaagg cagcgccgca
    75361 ccgccatggt gacgcaggat caggttttgc tccgccagct cattgaggtc gcggcgaata
    75421 gtctgcgggc tgacggagaa atgctctacc agctcttcag tactgacata accctgctgt
    75481 ttaaccagtt cgataatacc gttgtgacgt tgtgtttgtt tcatttataa atccctggaa
    75541 ttattttcgt tttcgcgcat tgagcgaatc aacaaaagcc atcgctaaac ccacggctaa
    75601 cccggcgatg tgtgctccgt tcgccatcga catcccaaac aaatcaaacc atccggcgac
    75661 aatccagatc agcgcaaaga taattaaccc acgttgcagg taaatgccac tttgcggatc
    75721 gcgttcacca cgtagccaga cgtagcccat cagcgcatac accacgccag aaagcccgcc
    75781 aaaccacggc ccgctgaatt tttgctgcac atagccgctt aacagggcgc taatgagagt
    75841 aataacaatt agcttaccgc taccgaggcg tttttccacc gcaccgccga gataccacca
    75901 ccagagcagg ttaaagagga tatgcatcag cgagaagtgc attaacgcgt gggtgaagta
    75961 acgccagaac tcaaatttca gtgttggatc gaatggccag gccagccata acatcacttc
    76021 ctgatcgccg agaatttgca tggcgataaa caccaccacg caggcgatca tcaccaccca
    76081 ggttaccgga cctgcgcgtt cacgcaaggc ggcaaagaaa ggataacggc gataatgcag
    76141 gccactgccg gtatggcctg cctgccagct ggccgccaga taacgcggat ctgccgggtt
    76201 ttcgagaaaa cgcgccagct ccgcccgtac gcgctcggcc tgggactcat ccgccagcca
    76261 gacatcgctt tggttatgtt gttgaatcgt gaggataaca ccctgcgtcg ccatgtaatc
    76321 aacaaacgcc tgcgccacgc gggggttagc aaaagaggta atcatcaaca ttgttgctgt
    76381 cgcttattcc acacaaaagg ggacagtata aagcgttacg cgccgtacgc cacctctgcg
    76441 ggaaactgac gttgccaggc ttcaaagccg ccgtcaatgc tatagaccac atcgtagccc
    76501 tgttgcagca gatactgcgc cgcgcctttg ctgctattgc cgtgataaca catcaccatc
    76561 accggagtgt caaagtcgtt atcacgcata aacgcgccca gcgtgtcgtt ggttaaatgg
    76621 aaagcctgca ccgcatgccc catagcgaaa ctctgtggat cgcgaatatc gaccagcacc
    76681 gcctcttttt cctgcaactt ctggtgcgcg tcggcaacgt taatacattc gaactgatcc
    76741 atgcgtctct ctttctttac aaacaagtgg gcaaatttac cgcacagttt acgtcgaagc
    76801 ggcagataaa cgccataatg ttatacatat cactctaaaa tgttttttca atgttaccta
    76861 aagcgcgatt ctttgctaat atgttcgata acgaacattt atgagcttta acgaaagtga
    76921 atgagggcag catggaaacc aaagatctga ttgtgatagg tggcggcatc aatggtgctg
    76981 gtatcgcggc agacgccgct ggacgcggtt tatccgtgct gatgctggag gcgcaggatc
    77041 tcgcttgcgc gacctcttcc gccagttcaa aactcattca cggtggcctg cgctaccttg
    77101 agcactatga attccgcctg gtcagcgagg cgctggctga acgtgaagtg ctgctgaaaa
    77161 tggctccgca tatcgccttc ccgatgcgtt ttcgcctgcc acatcgtccg catctgcgcc
    77221 cggcgtggat gattcggatt ggtctgttta tgtacgatca tctgggtaaa cgcaccagcc
    77281 tgccgggatc aaccggtttg cgttttggcg caaactcagt gttaaaaccg gaaattaagc
    77341 gcggattcga atattctgac tgttgggtag acgacgcccg tctggtactc gccaacgccc
    77401 agatggtggt gcgtaaaggc ggcgaagtgc ttactcggac tcgcgccacc tctgctcgcc
    77461 gcgaaaacgg cctgtggatt gtggaagcgg aagatatcga taccggcaaa aaatatagct
    77521 ggcaagcgcg cggcctggtt aacgccaccg gcccgtgggt gaaacagttc ttcgacgagg
    77581 ggatgcatct gccttcgcct tatggcattc gcctgatcaa aggcagccat attgtggtgc
    77641 cgcgtgtgca tacccagaag caggcctaca ttctgcaaaa cgaagataaa cgtattgtgt
    77701 tcgtgatccc gtggatggac gagttttcca tcatcggcac caccgatgtg gaatacaaag
    77761 gtgatccgaa agcagtgaag attgaagaga gtgaaatcaa ttacctgctg aaagtgtata
    77821 acacgcactt taaaaagcag ttaagccgtg acgatatcgt ctggacctac tccggtgtgc
    77881 gtccgctgtg tgatgatgag tccgactcgc cgcaggctat tacccgtgat tacacccttg
    77941 atatccatga tgaaaacggc aaagcaccgc tgctgtcggt attcggcggt aagttgacca
    78001 cctaccgcaa actggcggaa catgcgctgg aaaaactgac gccgtattat cagggcattg
    78061 gcccggcgtg gacgaaagag agcgtgctgc cgggtggtgc cattgaaggc gaccgcgacg
    78121 attatgccgc tcgcctgcgc cgccgctatc cgttcctgac tgaatcgctg gcgcgtcatt
    78181 acgctcgcac ttacggcagc aacagcgagc tgctgctcgg caatgcggga acggtaagcg
    78241 atctcgggga agatttcggt catgagttct acgaagcgga gctgaaatac ctggtggatc
    78301 acgaatgggt ccgccgcgcc gacgacgccc tgtggcgtcg cacaaaacaa ggcatgtggc
    78361 taaatgcgga tcaacaatct cgtgtgagtc agtggctggt ggagtatacg cagcagaagt
    78421 tgtcgctggc gtcgtaagga tgatgcccgg ttgtgaagtg caaccgggct atttggattc
    78481 cttagactat tcattgtaaa taattgaata acggcccctg agctaaaatc gccaaactta
    78541 agcacgttac ccaatcgccc cacttcttcc tctggtttct attttttctg caccaatggc
    78601 aataatatcc caatgcaaaa ccaagctgtg cactaatcgg gccgacaaaa tcagtccaag
    78661 gctgcggcca aaaaggggaa aaattcaggg gaagccagac aaacgtcaga aagaaatttc
    78721 ggatattttc cagcaacgct gatttctctg gagaaatata aagaatgaaa ttcgagagaa
    78781 ttaaccagaa atagaccggc gaaaagacca gccagcaatg cagtctgttt tttaattcag
    78841 gaggcgttct gcgggagaat tgccaaccga tgccagccat tacactgaaa aaaagtgcat
    78901 attcaaccat taactcaagt tgtagttcct gaagagaaat acgcacaacc aggagcggaa
    78961 aggagaatat tatttctgcc agaaaagtta acgtgaatat aagtacggct aagacaataa
    79021 ccgtcggcca taccaataat ggcacgataa caaaaccgag aattacaggg ggaatataaa
    79081 gctggaattt aatcattttt attttcctgg tgattcggat gatgcgtcat acatcgcgca
    79141 taaacactca tcaattatcc agtcgtctac ccagacctga tgctgtgtgc cctgttctgg
    79201 ctgctttggt gggttctcaa tcgcctgccg cgcgcgttct ttcaagatgg attgattgtt
    79261 gaaggcccag gcattgattt gggtggtaat gacgttatcg atatcgtcct ggttatctct
    79321 gatataatcc tcacaatatc tgttcaataa acaggcaaga gaaacggtcg accaaaagcc
    79381 gggaactgaa ttaatggcat ccagcaatgg cgcaagagcg atatcggcac ggaaaaacat
    79441 ttccagcata tactgtaatt cgacagcttc atcgccggat acccaatcag tcatatactg
    79501 gcaggcaaat cgttgcaaaa ttttccattc atcctcacgc caattttctt tactggcaag
    79561 gtttatacgc cgtagagaaa atacttcgtg aatattgtca aactgcccct gtgcaacaaa
    79621 ttccagtaaa cgcggcagaa aatagcggac ctcatcgcta ttttgcggat caggccctgg
    79681 gctactattc catgcattga ttaaggaata cgagatcgct cgtaacgatg tactccgtaa
    79741 taccttttgt tcctgctgac tcaggcaata ttcacaacaa accacaaatt tctgcggcac
    79801 cggataacga cgaaaaatat gataaatctg ctcaattaat gcctgcatgg cggggctggg
    79861 ttgcataatt accccctcgc ccgctgatcg cgtgtaaacg gttgataaac aaaacgagca
    79921 ttcgccacgt tcaggcgaat aatggcacta cgagagaggg aaaagctggg gcgaatgcaa
    79981 ctattgcagt ctcgacagct ttcttcagcg caattttcca ctgtgtaata acgagcgtag
    80041 agtttcagtt ccgcttgctt atcacgaaat ccgcgtaaac tgttatcgtt atgaaatttc
    80101 caggcatctt taaagacctg aaaaatgtag atatcctcaa tccaggactc acatttttta
    80161 agcgggcgct ttgctatgat cacccgctca tccgggttta gcatcgccgt aaattgaacg
    80221 gtgaggtcat ttgtcgtgtt cagatcacat tgaacatctg gtacatcact gatatagggg
    80281 tcaaatccaa agtcagcacc acaggcacaa agacggctaa tggtggcggt atcgagggaa
    80341 aatcccggcc atacattgct ggacgttaag ccaatattta atgtgacatt atatcgaagc
    80401 aatccctcaa gaccgtcagc accgacgaac ttttcccctt catccagggc ctggttaatg
    80461 agttgctgaa caatgccttc atcccagtat gccgatttgc agttggcaat cgtaatatcc
    80521 acgcctgtga agggatcagt accgacaagg cttgcaaata atgtattaac taattccatt
    80581 tttattatcc gccttaatgc aacgttttat acatcggcac ataacctaca tcttccacta
    80641 ggctctgccc ctgcggcgtt aaaaaccact ccaccaattt ttgtgtttct gacgtcatat
    80701 tttcccgcgt taccataaag gcatcgacga tatagggata tttgccgttg cgaatgtttt
    80761 cggcagtcgg tgcaataccg ttaatcgcta gcagttttat atttttatcg gcgttcattt
    80821 gtgttgcgta ataacggaag gtgtaaccta ttgcgttgtt tgtattacga tattcggcaa
    80881 cgactttaat cattccctcc attacgcttg ccacttcggt ttcctgcggc gagatcatac
    80941 ggacattttt catgacctgg gattgcatca ccgtctggct accagagtct tccgggcgtt
    81001 gccaggtctg gatctcctga tcgttaccgc caaccgtgcg ccagttggta attgcaccac
    81061 tgaagatgtc acgcacttgt tgttcggtca gggaattaac cgggttatct acattgacga
    81121 tgaaaacaaa cgcttcacgg gcaaaaggcg tgtaaatcaa agtgacgccc gattcctccg
    81181 cgcgtttttt ctgcccaccg gaaggttgag ccacaaagat aatatcggca ttacctttaa
    81241 caatcttgtt atatgcttcg ggagtacgag agttcgccag atattcccat tcgtgaaaat
    81301 cttccggcaa aacgcttagt gcataaaagg cagaggcata aatggggtac gccgccgttg
    81361 cgccatcaag tcgcggccag ttttgcgtga actgaatttg cggtttacca cgcagtggtg
    81421 tcagttgatt attgagttta tcgggtcgcc aggcccaggt atcaatattt tcacgcacac
    81481 cgacgccggg attaagctgt ttatataaat aagcctggta accggcgaca aaaccaagca
    81541 tgagaaggat aaacaagaga gaattacggc aacgtaaccc ggacgtgccc gttaaaggat
    81601 atctggtcgg ccagccgtat cccagcgaaa aactgatatg cgcgccaaca ggtataatga
    81661 ctaaccacca gtactcacca ccgactaatg aggctaacaa aacgcccagc caggggagca
    81721 taagatcagc caaccaaccc gctcccatct ggcgaaattg ataatctgcc agatgcagcg
    81781 cgattatcca gacccccagg gttaataatg cgggtactaa aaaaagacat tgtcgccaga
    81841 cggctatttc tcttttttct ggtgcactat cgtccgccca cactcgcccc gagaaaaata
    81901 gcattaatgc gcccagcgcc agcataaaaa tgttgagcag aaggtaaatt gttggcgtga
    81961 taaagatatt acaaacttca ataataccgg caagtccgac acccagcatg gcaataaccg
    82021 ccgccaaaaa ctgcgccaga atggggatgc cgaaaaaagt cattaccagc gaggtcaaaa
    82081 tccattttct gttttgcatt attctttcca ttctttctga atggcgaaat tatactcccc
    82141 gagtcccctt gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccctt
    82201 tggccccgtt ctatttatta gtgaaattac aatctcaccg gatcaatatg ccagatatga
    82261 tcggcgtact ctttgatagt acggtcagaa gagaagtagc ccatattggc aatgttcagc
    82321 atcgcttttg cggtccactc ttcctgaagc tcgtagagtt cgtcgacttt atcctgacaa
    82381 tcgacatagc tgcgataatc cgccagaacc tgatagtgat cgccgaagtt gatcagcgaa
    82441 tcaaccagat cgcgatagcg acccggatct tccggactga atacaccgct gccgatttgc
    82501 gtcagcacct gatgcagctc ctcatctttc tcgtagtatt cacgcggttt gtagccctga
    82561 cgacgcagtt cttccacttc ttccgcagtg ttaccaaaga taaagatatt gtcagcaccg
    82621 acatgatcca gcatctcgac attcgcaccg tccaacgtac cgatagtaag cgcaccgtta
    82681 agcgcaaact tcatgttact ggtgccggaa gcttccgtcc ccgccagcga aatctgttca
    82741 gacagatcgg ccgccggaat gatcaactgc gccaggctaa cgctgtagtt cgggatgaac
    82801 acgactttca gcttgtcgcc aatctgcgaa tcgttgttga tcactttcgc tacgtcattg
    82861 atcaaatgaa taatgtgctt cgccatgtaa taggccgaag ccgccttacc gccaaaaata
    82921 ttcacgcgcg gtacccactt cgcatccggg tcggccttga tgcggttata gcgggtaatc
    82981 acatgcaaca cattcatcaa ttgacgtttg tattcgtgaa tacgtttgat ttgtacatcg
    83041 aacaacgcct ttggattcac caccacattc agctgctggg cgatatactc tgccagacgc
    83101 tttttgttct ccagcttcgc ctgatgcaca gcgtgattaa ccattgggaa atcacagtgt
    83161 tgttgcagct cattaagcag gctaaggtcg gtgcgccagt tacggcccag gtgttcgtcc
    83221 agcacggctg aaagcgatgg gttcgctacc gctagccagc gacgcggcgt cacaccgttg
    83281 gtgacgttgg tgaaacgacc cgggaagatt ttcgcaaagt cggcaaacaa cgattgcacc
    83341 atcagattag agtgcagttc cgatacaccg ttaactttgt ggctcacaac aaccgccagc
    83401 caggccatac gcacacgacg accgttggat tcatcaatga tcgacgcccg tcccagcaga
    83461 tcggtatcgt tcggatactg ttcctgcaag gttttcagga aatagtcgtt gatttcaaag
    83521 atgatctgca ggtgacgcgg cagaatttta cccagcatat caaccggcca ggtttccagc
    83581 gcctcgctca tcagcgtgtg gttagtgtag gagaagacct gacaacacac ctcaaacgcg
    83641 tcgtcccagc taaattggtg ctcatcgatc agcagacgca tcatctcagg aatcgacagt
    83701 accggatggg tatcattgag atgaatcgcg attttatccg ccaggttatc gtaggtttta
    83761 tgcaactgat aatggcggct taaaatgtcc tgaatggtcg aggaaaccag gaagtattcc
    83821 tgacgcaggc gcagctcacg cccggagtag gtggagtcat ccggatacag tacgcgagat
    83881 acgttctcgg agtggttttt atcttccact gccgcgaagt agtcaccctg gttgaattta
    83941 ccgaggttaa tttcgctact ggcttgcgca ctccacaaac gcaacgtgtt ggtcgcgtcg
    84001 gtgtcgtaac cagggattat ctgatcgtaa gcgactccca gaatctcttc ggtttcaatc
    84061 cagcgcgttt ttttaccttc ctgctgaatg cgaccaccaa aacggacttt atagcgcgtg
    84121 ttgtggcgtt tgaattccca cgggttaccg tattccagcc agtagtctgg cgactctttc
    84181 tggctaccgt taacgatgtt ctgcttgaac ataccgtagt catagcggat gccgtaaccg
    84241 cgccccggca accctaacgt cgccagagaa tcaaggaagc aagccgccag acgtcccagg
    84301 ccaccgttac cgaggcctgg gtcattttct tcatcaatca gctcttcgag atttaacccc
    84361 atcgcttcca gtgcgccctg tacatcttcg taaattccta gcgacaacat ggcgttggag
    84421 agcgtacggc caatcaaaaa ctccatcgac aggtagtaaa cctgacgagt ttcttgcgac
    84481 aactgggcac ggtttgaacg taaccagcgc tccacgagac gatcgcgcac agcaaataac
    84541 gttgcgttca gccattcatg tttattggcg acgaccgggt cctttccaat cgtaaacatc
    84601 agcttgtaag cgatagagtg cttaagagct tctacgctaa gcgtgggcga tgaatatgta
    84661 aacggagcat tcatataggc gtttcctgaa aactatttca agcgatagta aagctcacgg
    84721 tacgacttcg ccgcgacctg ccagctaaaa tccattgcca tagcctgacg ttgcacaaac
    84781 cgccacagtg aaggacggga ccacagtaca aaagcacgtc gaatagcccg taacagcgac
    84841 caggcattac tatcttcaaa gacaaaccca ctggcgacgc catctgcaag gttctcaaga
    84901 gaacagtcag aaaccgtatc agcaagccca ccggtgcgcc gcactaacgg cagcgtaccg
    84961 tacttcaatc cataaagttg cgttaagccg cacggttcaa aacggctggg caccagaatg
    85021 acgtccgcgc cgcccataat gcgatgcgaa aatgcttcgt gatagccaat ctgaacgccc
    85081 acctgaccgg ggtattccgc tgccgccgca aggaaacctt cctgcagcac cggatcgccc
    85141 gcgccgagta gcgccagctg cccgccctgc tccagaagac ccggtaaggc ttccagcacc
    85201 aggtcgagac ctttctggct ggtcagacgg ctcaccactg caaaaagcgg cactttatcg
    85261 tcaaccttaa gccccattgc gatttgtaac tggcgcttat tttccgcttt atcttccaac
    85321 gtatcgcggg tgtaacgcga ggccaacagt aagtccgtct ctggactcca gattttctcg
    85381 tccacgccgt tcagtacgcc ggaaagacgc ccttcacggt gacgctgttg caacagacct
    85441 tccataccgt aggcaaactg cggttcggtg atctcgcgag cgtaggttgg actgaccgcc
    85501 gtaatgtgat cggcatagta cagaccggcc ttcaggaaag agatttgtcc gttgaattcc
    85561 agcccatgaa tattaaagaa tgaccatggc aattggatgt cattcatatg atgtgcataa
    85621 aacatgcctt gataggccag gttgtgcaca gtaaacaccg acttcgccgg acgcccgcgc
    85681 gccgccagat acgcaggcgc aaggcctgca tgccagtcgt gcgcatgcac cacatcagga
    85741 cgccagaatg ggtcaagccc gctggccatt tctgccccaa cccaccccag cagcgcaaaa
    85801 cgcaatacgt tgtcggtata ggcaaataag ttggtatcgt gatacgggct tcccggacga
    85861 tcatagagat gcggcgcgtc aatcaggtaa atgccaaccc cgttgtaatg accgaacaac
    85921 agcgtgatat gtccggcgaa ggtatcacga cgggatacta cctgcgcatc ggtcacgcca
    85981 cggcgaatat cgggaaatgc aggcaacagt acgcgagcgt caacgccgtc tgcgatttgt
    86041 gctgcgggta atgccccaat aacatcagcc agaccgccgg ttttaagcag cgggaacatc
    86101 tctgaacata catgtaaaac ctgcattatc gctcctgttt atgccctaac ttccgtagca
    86161 tttcgcgcgt taccagcacg atgccttctt ctgaacgata gaaacgacgt gcatcttcct
    86221 ctgcgttttc accaatcacc atgccttccg gaataacaca agcacgatcg atgacgcagc
    86281 ggcgcagacg gcacgagcga cctacccata cttccggtaa caatacggcg gaatcaatgt
    86341 tgcagaatga attcacgcga acgcgcgaga acagaacgga ctgcaccacc accgaaccgg
    86401 agatcacaca accgccggaa accagtgagt taagggtcat cccgtggcta ccggagcgat
    86461 cctgcacgaa tttcgctggc ggtaatgatt cattgtaggt gcgaattggc caattgcgat
    86521 cgtacatatc cagttccggc accacagagg ccagatcgag gttcgctttc cagtaagctt
    86581 ccagcgtacc cacatcgcgc cagtacggct cggcatccgg gtcggattgt acgcaagaga
    86641 gcgggaacgg gtgcgcatag gccagaccgg cttcggtgat cttgggaatc aaatctttgc
    86701 caaagtcgtg gctggagttc tcatcgcgat cgtcttcttc cagcagttca tacagatagt
    86761 cggcgtcaaa gacgtagata cccatactcg ccagagattt gctcggatcg ttcggcattg
    86821 acggcgggtt agcaggtttt tcaacgaatt cgataatttt atcgttctca tcaaccgcca
    86881 taacgccaaa tgcggaggct tcttcaatcg gtactggcat acaagcaacg gtgcaacgtg
    86941 cgcctttttc gacgtgatcg ataagcatac gcgagtagtc ttgcttgtag atatggtcgc
    87001 ccgccaggat caccacgtat tccgctttat aacggcggat aatgtcgagg ttttgggtga
    87061 ccgcatctgc ggtgccgcga taccagtttt cccctttcat tctctgctgt gctggcagca
    87121 gatcgacaaa ctcgttcatt tcttcattga agaatgacca gccgcgctga atgtgctgca
    87181 ccagagtgtg ggactggtac tgggtgatca cgcccatacg acggatcccg gagttgatgc
    87241 agttagacag cgcaaagtcg ataatgcgga acttaccgcc gaagtgtacg gccggttttg
    87301 ctcgcttatt ggttaaatcc ttcaggcggg taccacgtcc tcccgccagt atcagggcaa
    87361 cagatttcaa tggcagctgg cgcgccaaca ttaagtgatc gttcttctct aaactaacca
    87421 tgactaactc cttttttatc atctctggaa cacacacaat ccgtgtgcag gtccctgcca
    87481 gacagccgta atcactgggt tatcctctcc agcgaatggg ggaatggcgt gccactcccc
    87541 agcaggtaaa acaatctctg ttacctcaag cgtggcgtta attgcgatca aaaagcgatc
    87601 cgagagcaga atttgcagct gtttcggccc gttttgccac tcatccgtgc ttaaaggttg
    87661 agcatatcga tttagccaac ggacattgcc gtcgccttct tcccaccagc gattctccac
    87721 caaagcggga atgcgcttgc gcagatggat taacgcggcg gtaaatgcgg ttaaaccact
    87781 gcttgcctgc gaccagtcca accaggttaa ttggttatcc tgacagtagg cattgttatt
    87841 gccatgctgg ctgtgaccat gttcgtcacc ggccagtaac atcggcgtac cctgggagag
    87901 caacaacgtt gttaacaggg cgtgaatgct gtcgcgccgc cgttcaacca ggtcaagaga
    87961 accgcctaac ccttctttac catgattgtt actgtaattg ttgttggtcc cgtcgcgatt
    88021 ttcttctccg tttgcttcat tgtgtttatg gttgaagcaa acgcagtcgc gaagcgtaaa
    88081 accgtcatgc gcggtgacga gattaatcgc ggcactcggc agacgaccat tacgtttaaa
    88141 aacatcgctg gaggcagcaa aacgcccggc aaacgccccc agaggcaaat cataatgtag
    88201 ccagaaacga cgggcagcat cgcggaaatg atcgttccac tcggcaaaca gcggcgggaa
    88261 atttcccacc tgataaccac caggagcgat atcccacggt tcagcaatta acttcacctg
    88321 cgagagcacc gggcagttct ggatagcggt aaacaacggc gcatcctgac ggaactctgg
    88381 cgtacggccc atgactgccg ccagatcaaa gcggaaacca tcgacgtggc aggtttctac
    88441 ccaataacgc aggcaggcgc tggcataatc caccaccgcc ggatgactca aattgagcgt
    88501 gttgccgcaa ccggtccagt tgtgataatc gccgtcttct cttatccaat aatagctacg
    88561 gttatcgatc ccacgcagcg agaataacgg gccgtcgagg tccagttccg cactatggtt
    88621 gagcacgata tcaagaatga cttcgatacc cgctttatgc agtgctttga ttgcatcgcg
    88681 aaactcatcc agcgccgttt ctggcgagca ggcatacgcc ggatgcagcg caaacatcgc
    88741 caccgggttg taaccccagt agttacttag ccccatgcgt tgcagacgtg gttcactggc
    88801 aaactgcgcc actggcagca gttccagcgc ggtaatgccc aattgtttca aatagttgat
    88861 catcaccgga tgcccgaggg ctttataagt gccacggatc tcgaccggga tctccgggtg
    88921 caagtacgtt aatcctttga catgggcttc ataaatgatg gtgctgcccc acggcgtgcg
    88981 cggcggggca tcatcttccc agtcatagtg atcaaccacc actacgcatt tcggcgcaat
    89041 ggcggcgttg tcgcgatagt caggttcatt atgaccggcg tgcagcagcg ggttatcttt
    89101 aaactccccg tcaatttgcc gcgcgcaagg atcaatcaac aacttcgccg ggttaaagcg
    89161 atgcccctcg gcgggttgcc aggggccatg aacgcgataa ccataacgca aacccgggcg
    89221 cgcatccggc agataaccgt gccaaatgtc gccactgtgc cctggcaagt catagcgatg
    89281 ttcctggcca ttggcgtcaa agacacacag ttctacccgc tcggcatgag cggagaaaag
    89341 tgtgaagttg acgccctgac cgtcgtaatg cgcgccgagg ggagcgggtt tgccaatggc
    89401 gagttgtgtc attctgcctc ccgaaccagc cagatagtgg ccagcggtgg tagcgtcagg
    89461 cttagtgaat gctgacgacc gtggctggca atctcatcgc tgtgtaccgt gccgccattg
    89521 cctgcattac tgccgtgata gtgcatggaa tcggtattga ggatttcacg ccatttgccc
    89581 ggctggttta tgccgaagcg ataatcatga cgcggtaccg gcgtaaagtt actggcaacg
    89641 atgatttcgt taccctcttt atcgcgacgc acaaagatca gcaccgagcg ttctttgtca
    89701 tccaccacca gccattcaaa gccgtacggg tcaaaatcca gttcatgcat tgctttatgg
    89761 tggcggtagg tgaggttcag atcgcgcacc agacgctgga caccgtggtg ccagttatcg
    89821 ccgccttcca acagatgcca gtcgaggctg gcgtcatggt tccactcgcg gccctgggca
    89881 aattcgttac ccatgaacag tagtttcttg cccgggaatg cccacatcca gccatagtag
    89941 gcgcgcaggt tcgcgaattt ctgccatgcg tcgcccggca tgcggtcgag aatcgatttt
    90001 ttaccgtgga ccacttcatc atgcgacaac ggcaggacga agttttcagt gtagttgtag
    90061 agaatcccga aggtcagttt atcgtgatga tactgacgat aaaccgggtc gagcttcatg
    90121 tagtccaggg tgtcatgcat ccagccgagg ttccacttgt accagaagcc cagaccgccc
    90181 atatcctgcg gacgagaaac gccagggaaa tcggtagact cctcagccat tgtcaccgca
    90241 ccggaaacct gctcaccaag aatacggttg gtattacgca agaattcaat cgcttcaaga
    90301 ttctcgcgcc cgccaaattc gttcgggatc cactccccct ctttacggct gtagtcgcga
    90361 taaatcattg acgccaccgc atcgacgcgc agcgcatcaa taccaaaacg ttcaatccag
    90421 taaagcgcgt taccgacgag gaagttactg acttcacggc gaccatagtt gtagatcagc
    90481 gtgttccagt cctgatgata gccttcacgc ggatcgctgt gttcatacaa gttcgtgcca
    90541 tcaaattcgg caagcgcaaa gtcatcagtc gggaagtggc ctggcaccca gtcgagaatc
    90601 acgttcagac cagctgcgtg tgcggcatca atgaaataac ggaagtcgtc gcgagtacca
    90661 aaacggcggg ttggtgcata caggccggtt ggctgataac cccaactgcc atcgaaggga
    90721 tgctcgttaa tgggcagtag ttcgaggtgg gtaaagccca tccatttagc ataaggcacc
    90781 agttgatcgg ccagctcgcg gtagctcaac cagaaattgt tgtcggtgtg gcgacgccag
    90841 gaacccaggt gaacttcata aatagagatt ggcgcatcaa actgattcgc ttttttgcgc
    90901 tcttcagtct gtacaacctt ttccggcagc ccgcaaataa gagacgcggt ttccgggcgc
    90961 atttgcgctt cgaaggcata agggtcggac ttcagacgca agttgccatt ggcatcaatc
    91021 atctcgtatt tatagagctg accgttatgc gccccaggga taaacagttc ccagatgccg
    91081 ctctctttac gcaggcgcat cgggtgacgg cgaccgtccc agtagttgaa ttgcccaacc
    91141 accgagaccc gacgggcgtt tggagcccag acagagaaac gcgtacctgt gacgccatcc
    91201 atagtatctg catgcgcgcc taaggtttca tacgggcgca ggtgagtacc ttcagataat
    91261 agccaggcat ccatttcctg gattagcgga ccaaaacggt aaggatcatc aatcaggttt
    91321 tgctgaccat gccagacaac agccaactga tagcggaaaa aattcttacg tcgcggaatg
    91381 acgccgctaa agaatccccg tgagtcgaga cactccagtt ttgcgagttt gcgcccggtt
    91441 ttcggttcaa tcacccacac atcggtagcg tcgggtaaaa gggcacggac ttccagtccc
    91501 gcggtggttt tatgcattcc cagtacggaa aaaggatccg caaaatggcc tgcaattagc
    91561 gcgttaatca cgtctctatc gatacgatcg gacatgcttg tcttcctgtt ttattgtgtc
    91621 accccatcca attggatttt tgacttctgg ttgtgacatt tttttgacct gaacggcgca
    91681 gcactctgtg catcctctct gcgtcgtcct cacttcaggt aaggctgtga atactcatgt
    91741 attcagccac ccttaaagaa tagccaatgc tctatttaac tcccggtaaa tcatgaaaca
    91801 tctgcgctta ctcctgtatt acgcactaac aggggcggca tcgcgcccca gatttaatga
    91861 ataaagatta cgccagttga cgaagcatcc gacgcagcgg ctccgcggcc ccccacagca
    91921 gctggtcgcc cacggtaaag gctgacagga actctggtcc catattcagc ttacgcaggc
    91981 ggcctaccgg cgtggtcagc gtgccggtaa cggcagctgg ggttagctca cgcatagtga
    92041 tttcccgatc gttcggaacg actttcgccc acggattgtg cgcagccagc agttcttcca
    92101 cggtcggaat agacacatct tttttcaatt taatagtgaa tgcctggctg tggcagcgca
    92161 atgccccgac acgcacacat aaaccatcta ccggaattac ggaagatgtg ttgaggatct
    92221 tgttggtttc cgcctgccct ttccactctt cgcggctctg accgttatcg agctgtttgt
    92281 cgatccacgg aatcaggcta cccgccagcg gcacgccaaa gttatccacc ggcagctcac
    92341 cgctacgggt taaggttgtg actttgcgtt cgatatcgag aatagcagag gacggggtcg
    92401 cgagttcatc tgccacatgg ccatacagat ggcccatctg ggttaataac tcacgcatat
    92461 gtcgcgcacc accgccggaa gcggcctggt aggttgcaac ggacacccaa tcaacaagat
    92521 cattggcgaa taaaccaccc aacgacatca acatcaggct tacggtacag ttaccgccaa
    92581 caaaagtcct gatgccatta tttaatccgt cggtaatgac gtcctgattg acggggtcaa
    92641 gaatgatgat ggcgtcatct ttcatgcgca gagacgatgc tgcgtcaatc cagtaacctt
    92701 gccatccgct ttcacgaagc tttggataga tttcgttggt ataatcgccg ccctgacagg
    92761 tcacaatgat atcgagggcc tttagcgcct ccagatcaaa ggcatcctga agtgtgccag
    92821 tggttccgcc aaaagacggc gcagcctggc caagctgaga agtagaaaag aagacagggc
    92881 gaatggcgtc gaagtcgcgc tcttcaacca tgcgttgcat gagaacggag ccgaccatac
    92941 cgcgccagcc gataaaacca acatttttca taagcgtttt ttcctgcaaa gatgtgtgct
    93001 gtataaatgt gccggtctcc tcttggcaca tctttcacca tacaaaaagc agccaaagtc
    93061 gcaagtgaaa ttaatcaatg atagcgaagc catcagtaat gcgacttatc ctgctttgtt
    93121 agcacgcaga aagtccgcgg caattatcag ggaatttgag ttatgaatga aatcatttct
    93181 gcagcagttt tattgatcct gattatggat ccgctcggaa acctacctat tttcatgtcc
    93241 gtactgaaac atactgaacc gaaaagacgg cgggcaatca tggtgcgaga gttgcttatt
    93301 gctctcctgg tgatgctggt gttcctgttt gcgggtgaga aaattctggc atttctcagc
    93361 ctgcgagcag aaaccgtctc catttctggc ggcatcattc tgtttctaat cgccattaag
    93421 atgattttcc ccagcgcttc aggaaatagc agcgggcttc cggcaggtga agagccattt
    93481 atcgtgccgt tggcaattcc gttagtcgcc gggccgacta ttctcgccac gctgatgttg
    93541 ttgtctcatc agtacccgaa tcagatgggg catctggtga ttgctctgct gctggcctgg
    93601 ggcggcacct ttgtcatcct gctacagtct tcgctatttt tacgtctgct gggcgagaaa
    93661 ggggtgaacg cacttgaacg cctgatggga ttgattctgg tgatgatggc aacccagatg
    93721 ttcctcgacg gcattcgaat gtggatgaag gggtaacgag gagagttttt cgaaaagaac
    93781 ccggtagcgt cacaactacc gggcgaacaa acttaactca acagctggaa cgcaatcatc
    93841 ccaacgatgg caccgacagt gccgaggatg gtttccatca tggtccaggt tttcagcgtt
    93901 tcggcttcgg tcgcgccggt aaatttaccg aacaaccaga aaccggcgtc gttaacgtgg
    93961 ctgacaacaa tcgaaccacc agcgatacaa atcgacagcg ccgccatttg cgcaccggag
    94021 tagttcagtt gttcaataac cggcatcacc agtcctaccg ccgttaaaca ggctacggtg
    94081 gcagaaccct gaatgatgcg cactgcagcg gccagcacga agcaggtgat ggctatcggc
    94141 aggcccatgc cagttaacgc ttcgcccagt gccggaccta cgccagagtc aaccagcacc
    94201 tgtttgaaca cgccacctgc accaatcacc agcagaatga tccccgccgg ttgcagcgcg
    94261 tgaccgcaaa tctccatcac tttgtctttt ggcatgccct gacgcattgc caggccataa
    94321 atcgctacca gacaagcaac cagaatcgcg gtaaacggat gaccaataaa ctcgaaccat
    94381 tcgtaagcgg tagagccttc tggcacaaaa cgcgcggcaa tggttttcag ccccaccagc
    94441 accagcggca acaggatcag cgacaggctg aatccgaaag atggcatttt gccttcgccg
    94501 agatgcggtt cgctgatgtc gtcaggaata tgcaactcca cgtagcggct gatgaaatta
    94561 ccccacagcg gcccggcaat aatcattccc ggaattgccg cacacaggcc aatcaggatc
    94621 atccagccaa aatcggcgtt catctgcgat gccagcagca ttggcgctgg ccctggcacc
    94681 aggaacgccg ccgctgccgc cacgcctgcg aataatggga ttaccagctt caccaggttc
    94741 gtaccggtgt ggcgcgccat tgagaaagca acgctaatca gcagaacaat cgccacttca
    94801 aagaacagcg gtagcgcaca gaccagcccc gcaaggccga tggcataatg cgcgcggctg
    94861 tgaccgaagg atttaagcat tttgacggca atctgatcga ctgcgccggt ttcatgtaag
    94921 atcttgccaa acatagctcc cagggcgaca accaccgcca ggaagccgag ggtgcctccc
    94981 atcccttttt ccatcgtcgc tgcgatttta tcgagcggca taccggaaaa aaggccagcc
    95041 cccatggaca ccaccatcaa agccaggaaa gcgtgcatac gcgccttcat gactaaaaac
    95101 agcagcagta aaacagaccc tactgctgtt aaaacaagcg ttaatgtagt cactacttat
    95161 ttgccttttt taataacctc aatggtgctt gccacaacac cttccagcgg ttgatcgata
    95221 tccaccacca gtacatcagt ttcgtccgca cccggctcct gcagcgtttc aaactgcgtc
    95281 accaacattt gggttttaaa gaaatggcct ttgcgcgctt tcaggcggct ttcaatcacg
    95341 tcaaaatcgc ctttcaggta gatgaaagag agattcggat taccttcacg cagcaagtcg
    95401 cgatagtgtt ttttcaatgc agaacagacg atcagtgaca ctttattagt gcgctgcata
    95461 gcaaacgcgg cgtcgttcag cgcctgcaac cacggtttgc gatcgtcgtc attcagcggt
    95521 tcgccagacg ccattttttc gatattgcgc cgtggatgga ggaaatcgcc atcaagaaac
    95581 gcggcatgaa gttgatgcgc cacttcactg gcgaccgcag atttgccgct gcccgatacg
    95641 cccatcaaga cgtaaatgtg gtgatcatgg ttagtcgtgc tcaaagtggt gcccccacaa
    95701 tacaagaatt aaaaatgtta cgggtaactg ttatcggtaa cattgtccag ccggacaata
    95761 tcagaagcaa tatccatccg tgccttaagt gtataagtgt gagctacttc aaatttgtag
    95821 gcttaaatag atccgcccgg tgacaaggtg aaacctaaat ctaacatttt cggtgtcaca
    95881 gattcgccac gaatacgcgc cagcaggcgt tcagcgccaa tactgcccat ccgctcacgc
    95941 ggcgtcagca cgctcgctaa ccgcggctcc atcacctggc caatgtcatg accgtggaaa
    96001 ccggcaatcg ccatatcgtc aggaaccttc aatcccagac gctgacattc aaacgccgcg
    96061 ccgaccgcca ggtcatcgtt ggtacagaac acgccatcca gctgcggata ttcccgccgc
    96121 gcctggcgaa tcagttcaat accggaagag taagaagaag attgctcaac catcacgcta
    96181 tacggcacca gacctgcatc cagcatcgcc tgttcgtatc ccttctgttt gatgatagta
    96241 cgttcgtcga gacgcgcgcc gagataggca atgtggcaat gcccgcgagc aataatggcg
    96301 gtggtcatct ggcgtgccgc ttcaaagtta tcaaaaccga cggcgatatc gaggcacggc
    96361 gactggctgt ccatgagttc caccaccggg atccccgcca cttcaatcat ctttaaggtg
    96421 cgcggcgtgt gggtacgttc ggtgaggatc agtccgtcga tattccatga gagcatggat
    96481 tcgagacgtt cttgctccat ttccggttta taaccgtagt gcgccagcat ggtctgataa
    96541 ccgtgcgcgt cggtgacgct ttcgattccg cgtaatactt ccgcgaaaac ctggttggtg
    96601 agagaaggta acaggacgcc aatcgcccgg ctggtggcgt tagagaggat atcgggcgca
    96661 cgattgggaa tatagcccag ttcatcaaga gcggcggcaa tcttgccgcg tagagcgacg
    96721 gaaacctgct ccgggttgcg taaaaaacgg ctgaccgtca ttttggtcac gcctacacgg
    96781 tcagccacat cctgaagtac gggtcttttc tttttcatcg tcctgaaggt acaaaagaga
    96841 tagattctct tagtttaaca cggacgcatc agaaccttcc cggggaaaac agtgcttgtt
    96901 tacaattatt agatatacac taaatcaatt gcatcgcatt gtgctaacga cgcggcaaaa
    96961 ccccggaagc gtgcatcagc acgcgaccgg agcgagcgag gaagcctgca aaaaggcagg
    97021 cttcaaaaat gccgtggagc tttaaaccgg cggcagatcg aacagtaaca cttcgctgtc
    97081 gctatccgca tggatggaga ttgcctgctc atcccagatt gccagaccat cgctggtcga
    97141 ggctttcacg ccgttaatgg tgacattgcc tttcaccacc tggatccaga cgcggcgttc
    97201 agcggcaatc tgatgcaccg actgctcatc tttcagcaac gcccaacgat acagttccat
    97261 atcctgatgc actttcagcg aaccatctcg cgcatccggc gagagcacca gctgtttgcc
    97321 ctgcacggca tcgaagcgac gctgttcata acgcggcgta ataccgtttt cttcaggcat
    97381 gatccagatc tgatacagat gcagacgctc ggtgctgctt gggttgtact ctgagtgacg
    97441 aatacccgta cccgcactca taatctggaa ctcacccgcc ggaacctgct ctttattgcc
    97501 catgctgtcc tgatgctcaa cagtaccttc cagcacgtag gtcaaaattt ccatatcttt
    97561 atgcgggtga gtgccgaagc cctgccctgc ttcaatcacg tcgtcgttaa tcacgcgcag
    97621 cgcggagaag cccataaagt tcggatcgta atagttggca aaagagaaag tatgccagga
    97681 gtccagccag ccatgatttg catgaccacg ttcatttgct ttgcgtaagt agatcatttg
    97741 tattcgcccc ctgaatgatt tcgatgggct tagtgtggac ccgatctgcc tgggatgata
    97801 gagggtgaaa attgacccct ctgttcaaaa aaattgaact attcgagggg ccatcaaact
    97861 taattcgcga gagtcactgt ggagggagag gcttgctcaa atccgcgttc aaggatttcc
    97921 agattggtaa gaacttcaga ttccttgacg taatttggcg caccgtgggt gatggtttga
    97981 tacaactcat cataaacgcg cccgtaatcg cccatctccg gcttcatctc ttctctgacc
    98041 gtcacgccct cgtcattgac atactccagc acaccgaccg aattatccgc tgcgaatccc
    98101 ggttcgcccg gcataatatt agccttcagg ctggtttcct gctggtcgat accgtattta
    98161 ataaacgaac ctttcttacc gtgaacgata aatttcggat aatcgatttt caccagatgg
    98221 ctggttttga cgatggcttt caggtcgcca taaaacagtt gcgcttcaaa ggtgtcgtca
    98281 ggattggctt tattacgcag gctgcggatg tcataagcga cgtgatccgg gcgaccgaac
    98341 agagaaataa tctggtccat cgtatgcaca ccaaggccat aaaacgcgcc atcctgcggc
    98401 agcccaggtt tggtctctgc caccgggcgg taatagtcaa aatggctttc cacttcaaca
    98461 atctctccca acttgccact ttcaatcgct tttttcgctg tcaggaagca ggagtcaaag
    98521 cgacgattct gatacggcgt gacggtcagc cctttgcttt tcgccaacgc aaacagctcc
    98581 ttcgcctggg caagcgtcgg ggtgaacggt ttttcgacca gcacattttt cccggcttcc
    98641 agcgcgcgtt tcgcgtactc gaaatggctg tccgcgtggg tgcagacaac aaccagctta
    98701 acatcgggat cgtttagtac ttcgtcgaga tcgctggtga aatggatatg ggaataaatg
    98761 ggagcctgtt cttccggctt cgcatggcga cgaaaaatat gcgcgacatg ccagctatcc
    98821 ttgcggttaa gtacatacgg cagatggtaa cgggtggtgc ttttgccgaa gccaataaag
    98881 gcgcagttga tgaccatgat ttcgtccttt ttaaggtggt tatttacacc ttagcgcaaa
    98941 gcggacgtgg ttcctactgg agtgcgcata acgaacacaa gcacttcccg aggataaatt
    99001 gagaacgaaa gatcaaaaaa aaagccagca cccggctggc taagtaatac tggaagcaat
    99061 gtgagcaatg tcgtgctttc aggttctccg cgagggtctt cctgatcgcg agacaataat
    99121 aatcattctc attcgcactt gtccaacact ttttgcaaaa aaatgcattt gactcgcatt
    99181 tgaaagtcaa tgatgttgaa agggacattt accccaaaga ggacaaagga atgagtgaga
    99241 tagtaatacg ccacgcagaa acacgggatt acgaggccat caggcagatt cacgcccagc
    99301 cggaggtgta ttgcaacaca ctacaggtgc ctcatccttc cgatcatatg tggcaggagc
    99361 gactcgccga tcgtcccggc atcaagcaac tcgtcgcctg tattgatgga gacgtcgtgg
    99421 gccatctcac cattgacgtg caacagcgcc cacgccgcag tcatgttgcc gattttggta
    99481 tctgtgtcga ctctcgttgg aagaaccgcg gcgtcgccag cgccctgatg cgagagatga
    99541 ttgaaatgtg cgacaactgg ttgcgggtag atcgcattga actaaccgtg tttgtcgata
    99601 acgccccggc aattaaggtc tataaaaaat acggctttga aattgaaggg actggtaaga
    99661 agtacgcatt gcgtaatggt gaatatgtcg atgcatatta tatggcgcgg gtgaagtaag
    99721 atagtgccct ttttctgaga tggaaaaagg gtgtcattca aaatcgacat accttc
//
LOCUS       DAEVZA010000002        98535 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEVZA010000002 DAEVZA010000000
VERSION     DAEVZA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 98535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.002"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(1..314)
                     /locus_tag="LAF92_000089"
                     /pseudo
     CDS             complement(1..314)
                     /locus_tag="LAF92_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            587..1382
                     /locus_tag="LAF92_000090"
                     /pseudo
     CDS             587..1382
                     /locus_tag="LAF92_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
     gene            complement(1453..1902)
                     /gene="rplI"
                     /locus_tag="LAF92_000091"
     CDS             complement(1453..1902)
                     /gene="rplI"
                     /locus_tag="LAF92_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBI9789160.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1944..2171)
                     /gene="rpsR"
                     /locus_tag="LAF92_000092"
     CDS             complement(1944..2171)
                     /gene="rpsR"
                     /locus_tag="LAF92_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBI9789161.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(2176..2490)
                     /gene="priB"
                     /locus_tag="LAF92_000093"
     CDS             complement(2176..2490)
                     /gene="priB"
                     /locus_tag="LAF92_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBI9789162.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(2497..2892)
                     /gene="rpsF"
                     /locus_tag="LAF92_000094"
     CDS             complement(2497..2892)
                     /gene="rpsF"
                     /locus_tag="LAF92_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBI9789163.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            3219..3494
                     /locus_tag="LAF92_000095"
     CDS             3219..3494
                     /locus_tag="LAF92_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI9789164.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3623..4309)
                     /gene="ulaF"
                     /locus_tag="LAF92_000096"
     CDS             complement(3623..4309)
                     /gene="ulaF"
                     /locus_tag="LAF92_000096"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBI9789165.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4309..5163)
                     /gene="ulaE"
                     /locus_tag="LAF92_000097"
     CDS             complement(4309..5163)
                     /gene="ulaE"
                     /locus_tag="LAF92_000097"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBI9789166.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5173..5823)
                     /gene="ulaD"
                     /locus_tag="LAF92_000098"
     CDS             complement(5173..5823)
                     /gene="ulaD"
                     /locus_tag="LAF92_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBI9789167.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5837..6301)
                     /gene="ulaC"
                     /locus_tag="LAF92_000099"
     CDS             complement(5837..6301)
                     /gene="ulaC"
                     /locus_tag="LAF92_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBI9789168.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6311..6616)
                     /gene="ulaB"
                     /locus_tag="LAF92_000100"
     CDS             complement(6311..6616)
                     /gene="ulaB"
                     /locus_tag="LAF92_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBI9789169.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6632..8029)
                     /gene="ulaA"
                     /locus_tag="LAF92_000101"
     CDS             complement(6632..8029)
                     /gene="ulaA"
                     /locus_tag="LAF92_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBI9789170.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8384..9448
                     /gene="ulaG"
                     /locus_tag="LAF92_000102"
     CDS             8384..9448
                     /gene="ulaG"
                     /locus_tag="LAF92_000102"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBI9789171.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9556..10311
                     /gene="ulaR"
                     /locus_tag="LAF92_000103"
     CDS             9556..10311
                     /gene="ulaR"
                     /locus_tag="LAF92_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBI9789172.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10308..11057)
                     /gene="yjfP"
                     /locus_tag="LAF92_000104"
     CDS             complement(10308..11057)
                     /gene="yjfP"
                     /locus_tag="LAF92_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI9789173.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11239..11568
                     /gene="bsmA"
                     /locus_tag="LAF92_000105"
     CDS             11239..11568
                     /gene="bsmA"
                     /locus_tag="LAF92_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBI9789174.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11717..11992
                     /gene="yjfN"
                     /locus_tag="LAF92_000106"
     CDS             11717..11992
                     /gene="yjfN"
                     /locus_tag="LAF92_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBI9789175.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12109..13734)
                     /locus_tag="LAF92_000107"
     CDS             complement(12109..13734)
                     /locus_tag="LAF92_000107"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBI9789176.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13818..14981)
                     /locus_tag="LAF92_000108"
     CDS             complement(13818..14981)
                     /locus_tag="LAF92_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBI9789177.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14984..15622)
                     /locus_tag="LAF92_000109"
     CDS             complement(14984..15622)
                     /locus_tag="LAF92_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBI9789178.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15632..16030)
                     /locus_tag="LAF92_000110"
     CDS             complement(15632..16030)
                     /locus_tag="LAF92_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBI9789179.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(16048..16707)
                     /locus_tag="LAF92_000111"
     CDS             complement(16048..16707)
                     /locus_tag="LAF92_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HBI9789180.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16758..17456)
                     /locus_tag="LAF92_000112"
     CDS             complement(16758..17456)
                     /locus_tag="LAF92_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HBI9789181.1"
                     /translation="MGILKSLFTLGKSFIFQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(17475..17876)
                     /locus_tag="LAF92_000113"
     CDS             complement(17475..17876)
                     /locus_tag="LAF92_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HBI9789182.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(18003..18734)
                     /gene="rlmB"
                     /locus_tag="LAF92_000114"
     CDS             complement(18003..18734)
                     /gene="rlmB"
                     /locus_tag="LAF92_000114"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBI9789183.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(18914..21355)
                     /gene="rnr"
                     /locus_tag="LAF92_000115"
     CDS             complement(18914..21355)
                     /gene="rnr"
                     /locus_tag="LAF92_000115"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBI9789184.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(21394..21819)
                     /gene="nsrR"
                     /locus_tag="LAF92_000116"
     CDS             complement(21394..21819)
                     /gene="nsrR"
                     /locus_tag="LAF92_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBI9789185.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(22024..23322)
                     /gene="purA"
                     /locus_tag="LAF92_000117"
     CDS             complement(22024..23322)
                     /gene="purA"
                     /locus_tag="LAF92_000117"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBI9789186.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(23426..23623)
                     /locus_tag="LAF92_000118"
     CDS             complement(23426..23623)
                     /locus_tag="LAF92_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBI9789187.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(23705..24709)
                     /gene="hflC"
                     /locus_tag="LAF92_000119"
     CDS             complement(23705..24709)
                     /gene="hflC"
                     /locus_tag="LAF92_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBI9789188.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(24712..25971)
                     /gene="hflK"
                     /locus_tag="LAF92_000120"
     CDS             complement(24712..25971)
                     /gene="hflK"
                     /locus_tag="LAF92_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBI9789189.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(26057..27337)
                     /gene="hflX"
                     /locus_tag="LAF92_000121"
     CDS             complement(26057..27337)
                     /gene="hflX"
                     /locus_tag="LAF92_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBI9789190.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(27413..27721)
                     /gene="hfq"
                     /locus_tag="LAF92_000122"
     CDS             complement(27413..27721)
                     /gene="hfq"
                     /locus_tag="LAF92_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBI9789191.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(27807..28757)
                     /gene="miaA"
                     /locus_tag="LAF92_000123"
     CDS             complement(27807..28757)
                     /gene="miaA"
                     /locus_tag="LAF92_000123"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBI9789192.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(28750..30597)
                     /gene="mutL"
                     /locus_tag="LAF92_000124"
     CDS             complement(28750..30597)
                     /gene="mutL"
                     /locus_tag="LAF92_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBI9789193.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIALWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(30607..31944)
                     /gene="amiB"
                     /locus_tag="LAF92_000125"
     CDS             complement(30607..31944)
                     /gene="amiB"
                     /locus_tag="LAF92_000125"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBI9789194.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(31963..32424)
                     /gene="tsaE"
                     /locus_tag="LAF92_000126"
     CDS             complement(31963..32424)
                     /gene="tsaE"
                     /locus_tag="LAF92_000126"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBI9789195.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(32396..33943)
                     /gene="nnr"
                     /locus_tag="LAF92_000127"
     CDS             complement(32396..33943)
                     /gene="nnr"
                     /locus_tag="LAF92_000127"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBI9789196.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            33942..35081
                     /gene="queG"
                     /locus_tag="LAF92_000128"
     CDS             33942..35081
                     /gene="queG"
                     /locus_tag="LAF92_000128"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBI9789197.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(35064..35117)
                     /locus_tag="LAF92_000129"
     CDS             complement(35064..35117)
                     /locus_tag="LAF92_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789198.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(35351..35426)
                     /locus_tag="LAF92_000130"
     tRNA            complement(35351..35426)
                     /locus_tag="LAF92_000130"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35391..35393),aa:Gly,seq:gcc)
     gene            complement(35462..35537)
                     /locus_tag="LAF92_000131"
     tRNA            complement(35462..35537)
                     /locus_tag="LAF92_000131"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35502..35504),aa:Gly,seq:gcc)
     gene            complement(35574..35649)
                     /locus_tag="LAF92_000132"
     tRNA            complement(35574..35649)
                     /locus_tag="LAF92_000132"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35614..35616),aa:Gly,seq:gcc)
     gene            complement(35860..36405)
                     /gene="orn"
                     /locus_tag="LAF92_000133"
     CDS             complement(35860..36405)
                     /gene="orn"
                     /locus_tag="LAF92_000133"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBI9789199.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            36500..37552
                     /gene="rsgA"
                     /locus_tag="LAF92_000134"
     CDS             36500..37552
                     /gene="rsgA"
                     /locus_tag="LAF92_000134"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBI9789200.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            37649..38617
                     /gene="psd"
                     /locus_tag="LAF92_000135"
     CDS             37649..38617
                     /gene="psd"
                     /locus_tag="LAF92_000135"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBI9789201.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            38639..41962
                     /gene="mscM"
                     /locus_tag="LAF92_000136"
     CDS             38639..41962
                     /gene="mscM"
                     /locus_tag="LAF92_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBI9789202.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(42113..43615)
                     /gene="yjeM"
                     /locus_tag="LAF92_000137"
     CDS             complement(42113..43615)
                     /gene="yjeM"
                     /locus_tag="LAF92_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBI9789203.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(43834..44811)
                     /gene="epmA"
                     /locus_tag="LAF92_000138"
     CDS             complement(43834..44811)
                     /gene="epmA"
                     /locus_tag="LAF92_000138"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBI9789204.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            45136..46944
                     /gene="frdA"
                     /locus_tag="LAF92_000139"
     CDS             45136..46944
                     /gene="frdA"
                     /locus_tag="LAF92_000139"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBI9789205.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            46937..47671
                     /gene="frdB"
                     /locus_tag="LAF92_000140"
     CDS             46937..47671
                     /gene="frdB"
                     /locus_tag="LAF92_000140"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBI9789206.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            47682..48077
                     /gene="frdC"
                     /locus_tag="LAF92_000141"
     CDS             47682..48077
                     /gene="frdC"
                     /locus_tag="LAF92_000141"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBI9789207.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            48088..48447
                     /gene="frdD"
                     /locus_tag="LAF92_000142"
     CDS             48088..48447
                     /gene="frdD"
                     /locus_tag="LAF92_000142"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBI9789208.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            48510..49643
                     /locus_tag="LAF92_000143"
     CDS             48510..49643
                     /locus_tag="LAF92_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBI9789209.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTTGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNHRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            49732..50265
                     /gene="blc"
                     /locus_tag="LAF92_000144"
     CDS             49732..50265
                     /gene="blc"
                     /locus_tag="LAF92_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBI9789210.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(50262..50579)
                     /gene="sugE"
                     /locus_tag="LAF92_000145"
     CDS             complement(50262..50579)
                     /gene="sugE"
                     /locus_tag="LAF92_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBI9789211.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(50754..50900)
                     /gene="ecnB"
                     /locus_tag="LAF92_000146"
     CDS             complement(50754..50900)
                     /gene="ecnB"
                     /locus_tag="LAF92_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBI9789212.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(51011..51136)
                     /gene="ecnA"
                     /locus_tag="LAF92_000147"
     CDS             complement(51011..51136)
                     /gene="ecnA"
                     /locus_tag="LAF92_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBI9789213.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(51188..51754)
                     /gene="efp"
                     /locus_tag="LAF92_000148"
     CDS             complement(51188..51754)
                     /gene="efp"
                     /locus_tag="LAF92_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBI9789214.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            51796..52824
                     /gene="epmB"
                     /locus_tag="LAF92_000149"
     CDS             51796..52824
                     /gene="epmB"
                     /locus_tag="LAF92_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBI9789215.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            53214..54083
                     /locus_tag="LAF92_000150"
     CDS             53214..54083
                     /locus_tag="LAF92_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBI9789216.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(54286..54639)
                     /locus_tag="LAF92_000151"
     CDS             complement(54286..54639)
                     /locus_tag="LAF92_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBI9789217.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(54777..56423)
                     /gene="groL"
                     /locus_tag="LAF92_000152"
     CDS             complement(54777..56423)
                     /gene="groL"
                     /locus_tag="LAF92_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBI9789218.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(56467..56760)
                     /locus_tag="LAF92_000153"
     CDS             complement(56467..56760)
                     /locus_tag="LAF92_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBI9789219.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            57036..58292
                     /gene="yjeH"
                     /locus_tag="LAF92_000154"
     CDS             57036..58292
                     /gene="yjeH"
                     /locus_tag="LAF92_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBI9789220.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWVGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(58308..58784)
                     /gene="fxsA"
                     /locus_tag="LAF92_000155"
     CDS             complement(58308..58784)
                     /gene="fxsA"
                     /locus_tag="LAF92_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBI9789221.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            59121..60557
                     /gene="aspA"
                     /locus_tag="LAF92_000156"
     CDS             59121..60557
                     /gene="aspA"
                     /locus_tag="LAF92_000156"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBI9789222.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            60675..61976
                     /gene="dcuA"
                     /locus_tag="LAF92_000157"
     CDS             60675..61976
                     /gene="dcuA"
                     /locus_tag="LAF92_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBI9789223.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFLLGSFML"
     gene            62092..62430
                     /gene="cutA"
                     /locus_tag="LAF92_000158"
     CDS             62092..62430
                     /gene="cutA"
                     /locus_tag="LAF92_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBI9789224.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            62406..64103
                     /gene="dsbD"
                     /locus_tag="LAF92_000159"
     CDS             62406..64103
                     /gene="dsbD"
                     /locus_tag="LAF92_000159"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBI9789225.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            64140..64715
                     /locus_tag="LAF92_000160"
     CDS             64140..64715
                     /locus_tag="LAF92_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI9789226.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            64822..64897
                     /locus_tag="LAF92_000161"
     tRNA            64822..64897
                     /locus_tag="LAF92_000161"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64855..64857,aa:Phe,seq:gaa)
     gene            65514..67052
                     /gene="cadC"
                     /locus_tag="LAF92_000162"
     CDS             65514..67052
                     /gene="cadC"
                     /locus_tag="LAF92_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HBI9789227.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPIKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            67418..68752
                     /gene="cadB"
                     /locus_tag="LAF92_000163"
     CDS             67418..68752
                     /gene="cadB"
                     /locus_tag="LAF92_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBI9789228.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            68832..70979
                     /gene="cadA"
                     /locus_tag="LAF92_000164"
     CDS             68832..70979
                     /gene="cadA"
                     /locus_tag="LAF92_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBI9789229.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGEVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            71038..72495
                     /gene="dtpC"
                     /locus_tag="LAF92_000165"
     CDS             71038..72495
                     /gene="dtpC"
                     /locus_tag="LAF92_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBI9789230.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            72732..74249
                     /gene="lysS"
                     /locus_tag="LAF92_000166"
     CDS             72732..74249
                     /gene="lysS"
                     /locus_tag="LAF92_000166"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBI9789231.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(74368..74541)
                     /gene="ghoT"
                     /locus_tag="LAF92_000167"
     CDS             complement(74368..74541)
                     /gene="ghoT"
                     /locus_tag="LAF92_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HBI9789232.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(74569..74865)
                     /gene="ghoS"
                     /locus_tag="LAF92_000168"
     CDS             complement(74569..74865)
                     /gene="ghoS"
                     /locus_tag="LAF92_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBI9789233.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            complement(75092..75364)
                     /locus_tag="LAF92_000169"
     CDS             complement(75092..75364)
                     /locus_tag="LAF92_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI9789234.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            complement(75376..75606)
                     /locus_tag="LAF92_000170"
     CDS             complement(75376..75606)
                     /locus_tag="LAF92_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBI9789235.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            75787..77418
                     /gene="dcuS"
                     /locus_tag="LAF92_000171"
     CDS             75787..77418
                     /gene="dcuS"
                     /locus_tag="LAF92_000171"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBI9789236.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            77415..78134
                     /gene="dcuR"
                     /locus_tag="LAF92_000172"
     CDS             77415..78134
                     /gene="dcuR"
                     /locus_tag="LAF92_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBI9789237.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            78705..80045
                     /gene="dcuB"
                     /locus_tag="LAF92_000173"
     CDS             78705..80045
                     /gene="dcuB"
                     /locus_tag="LAF92_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBI9789238.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            80123..81769
                     /gene="fumB"
                     /locus_tag="LAF92_000174"
     CDS             80123..81769
                     /gene="fumB"
                     /locus_tag="LAF92_000174"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBI9789239.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            81892..82521
                     /locus_tag="LAF92_000175"
     CDS             81892..82521
                     /locus_tag="LAF92_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBI9789240.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(82660..84081)
                     /gene="melB"
                     /locus_tag="LAF92_000176"
     CDS             complement(82660..84081)
                     /gene="melB"
                     /locus_tag="LAF92_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBI9789241.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVAYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(84184..85539)
                     /gene="melA"
                     /locus_tag="LAF92_000177"
     CDS             complement(84184..85539)
                     /gene="melA"
                     /locus_tag="LAF92_000177"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBI9789242.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            85822..86730
                     /gene="melR"
                     /locus_tag="LAF92_000178"
     CDS             85822..86730
                     /gene="melR"
                     /locus_tag="LAF92_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBI9789243.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            86929..89196
                     /gene="adiA"
                     /locus_tag="LAF92_000179"
     CDS             86929..89196
                     /gene="adiA"
                     /locus_tag="LAF92_000179"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBI9789244.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            89521..90282
                     /gene="adiY"
                     /locus_tag="LAF92_000180"
     CDS             89521..90282
                     /gene="adiY"
                     /locus_tag="LAF92_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBI9789245.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            90419..91756
                     /gene="adiC"
                     /locus_tag="LAF92_000181"
     CDS             90419..91756
                     /gene="adiC"
                     /locus_tag="LAF92_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBI9789246.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            91860..93503
                     /gene="eptA"
                     /locus_tag="LAF92_000182"
     CDS             91860..93503
                     /gene="eptA"
                     /locus_tag="LAF92_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBI9789247.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            93500..94168
                     /gene="basR"
                     /locus_tag="LAF92_000183"
     CDS             93500..94168
                     /gene="basR"
                     /locus_tag="LAF92_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBI9789248.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            94169..95269
                     /gene="pmrB"
                     /locus_tag="LAF92_000184"
     CDS             94169..95269
                     /gene="pmrB"
                     /locus_tag="LAF92_000184"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBI9789249.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(95245..95334)
                     /gene="pmrR"
                     /locus_tag="LAF92_000185"
     CDS             complement(95245..95334)
                     /gene="pmrR"
                     /locus_tag="LAF92_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBI9789250.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(95446..96948)
                     /gene="proP"
                     /locus_tag="LAF92_000186"
     CDS             complement(95446..96948)
                     /gene="proP"
                     /locus_tag="LAF92_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBI9789251.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIASKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(97212..98090)
                     /locus_tag="LAF92_000187"
     CDS             complement(97212..98090)
                     /locus_tag="LAF92_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBI9789252.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(98087..>98535)
                     /locus_tag="LAF92_000188"
     CDS             complement(98087..>98535)
                     /locus_tag="LAF92_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBI9789253.1"
                     /translation="VISRQEATDEQSTRLGVVRETFSRWLNQPDWGRGNEKSPTETVD
                     YSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQTRLRESLA
                     LRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT        24460 a        25840 c        24123 g        24112 t
ORIGIN      
        1 ggggttttac ggcgcaccag ataaaccagg ccaagcgtat gctggttgat caaataatat
       61 cccccaagcg cagcaatatt aatattcacc tgcaatgggt cgatacctgg cttaaaaata
      121 ttgtatttct tcccttcatc caataaggat tccattaact gcaagtgcgc atggttaatt
      181 tctaagagac gctatgattt tgcataatgc acacctttac tttgattttc actatgcaca
      241 atttttaaaa accaggggtt agcaatataa taatcccatg taaaatcaat aattttctca
      301 atggcttttt gtggctggtt aaccagatga ttaacaaacg caccgatacc aacagcgtag
      361 aataattaca taccgcacaa caagaatgta tggtggaagg cgtggaagtc attttctccc
      421 cgggggcatc gttatatcca taatgagcga tttttgatat taatatcttt caccatccgc
      481 tattttcacc acttcagaaa acaaacttat ttttattaat atcaattctt tttctttgaa
      541 cattcaactg cgatatttgt aaaaatatta aacaggcaac aataaaatga cattaccaac
      601 aactatttat tcattccccg catatttacc tcgtttttcg tccaccgaca aaccagcaaa
      661 actaaaattt catcaatacg cacgcgctac attactgtca aatcgtggaa gagatcataa
      721 ttgcgatggc agacgtactg ttgagatcca caaactagat ctctctgact ggcaagtcat
      781 tcaataagtt agcaacgaag tgtaatgctt acgatggaat aacaatgaat ggtgataatt
      841 cttttggatg gaaccatgaa gctacattag ataatattca cgcccagaaa tacaataaag
      901 cttacgcggg tgcgcggtta accgcagaac tgaaatattt attgcaagat gtcgaatcct
      961 tcgaacccaa cagtaaatac acaatacacg aagtggtttt gggacctggt tatggtactc
     1021 cagactatac cggccaaacg ataggatacg ttgtaaccct tcctgcccaa atgcctaatt
     1081 gctggtcatc tgaactacca acaatcgatc tttatatcga ccaacttcgc actgttacag
     1141 gcgtttctaa tgccctcgga ttcatcattg ctgcattgct aaacgcgtat tctgatttac
     1201 cccatgattt gaaaattggt ctgcgttcct tatcaagttc agtcgcaatc tactcgggtt
     1261 tgggtttcga gcgagtgcca caggagcgag acatcagttg cgctcgtatg catctaacgc
     1321 cagcaaacca tcctgactta tggatacaag agaatggtga atggatctac ttgcgaaact
     1381 aatacctgac gaaaaataga aagcaaaacg ccgaccaatg gtcggcgttt ttacgtctcg
     1441 ttgaataacg aattattcag ctactacgtt tacgatcact ttcgcgaata cttcgctgtg
     1501 aacctggaag ctcacttcgt gttcgccagt ggtacgcaga acgccgttcg gcagacgaac
     1561 ttcgctctta gccacttcaa cgccagctgc agttacagcg tcagcgatgt cgcgagtacc
     1621 gatggaaccg aacagtttac cttcgtcgcc agctttagac gcgatggtaa cagtttccag
     1681 tgcattgatt ttctcagcgc gagcattagc agctgccaga acttcagcca gtttagcttc
     1741 cagttcagcg cgacgtgctt cgaagaattc aatgtttttc ttggtagctg gaacagcttt
     1801 accctgcggt accaggaagt tacgagcata gcccgcttta acgtttacct gatcacccag
     1861 gctacccagg tttgctactt tatcaagcag aataacttgc attaccttat cctctcaaag
     1921 tcgtattaat ggaccgtgac cgattactga tggcgatcag tgtacggcag cagggacagg
     1981 tagcgagcgc gtttgatagc gcgagccagc tgacgctggt attttgcacg ggtaccggtg
     2041 atacggcttg ggacaatctt accgctttcg gtgatgtagt ttttcagcgt agcgatatct
     2101 ttatagtcga tctcttgaac gccttccgcg gtgaaacggc agaacttgcg acgacggaaa
     2161 taacgtgcca tatggctagt ctccagaatc tatcaattca atctgctcgg catgcaaaac
     2221 cattttgctc agtccgttct ttgccttgtg gcatgaaatg aacccctgaa cggttatgcg
     2281 actgccgacc gttatactgt gagtaatggc ctggttttcg tgtccgctaa caataacggg
     2341 catttgacac catgcctgcc ggtgaaagcc ggcttcctcc tgcacagaac gatgctcaag
     2401 cacgaactgg cagtgaggaa ttcctgatgg actgaccttt cgaaggggag tcctgcacac
     2461 ggtgccggac aacaccagac ggttggtcat cagaaattac tcttcagaat ccccagcttc
     2521 agcatcatca gcggtttcgt ttgcgaaatc atcgcgacgc tcacggcgct cgtctttcgc
     2581 tttaaccatc ggagatgctt cggtaacagc gtgcttggta cgcataacca tgctgcggat
     2641 aacggcatcg ttgaagcgga aggtagtttc cagctcatcg atcacttcct gcggagcttc
     2701 aacgttcatc agaacgtagt gtgctttgtg cagtttgttg atcgggtaag ccagctgacg
     2761 gcgaccccag tcttccagac ggtggatctt gccttctgca ccagtgatgg cagcagtgta
     2821 gcgctcgatc atgcccggaa cctgttcgct ctggtcagga tggaccataa aaacgatttc
     2881 gtaatgacgc atcgaattgc tccttacgga ttattcagcc tcctgtctgg gtcagccgaa
     2941 tcccggggag gcaaggaacg tgttaaaggt cggctgaaaa atgacgcgtt atagtacttg
     3001 tatgccccgc taaactcaag gccagccgac aaataaatct catctttcgc tcaaaagaac
     3061 taactccctg atttgaagtt catcacattc cgtcacagtg ttttttcgaa caactgcatc
     3121 agaaatggtc accatcaact tctttatcat tgattaaaat taaatcagaa gatcatcaca
     3181 gaggagagca cacaaagtca gccgtcgagg aggtatctat gttcagtcga gttttagccc
     3241 ttctggctgt gcttttgcta agtgcaaata catgggcagc cattgaaatt aataaccacc
     3301 aggccagaaa catggatgat gtgcaaagct taggcgtgat ttatatcaat cataatttcg
     3361 ccactgaaag cgaagcgcgt caggcattaa atgaagagac agatgcgcag ggcgcaacgt
     3421 actaccacgt aattctgatg cgggaaccgg ggagtaacgg caatatgcac gccagcgcgg
     3481 atatttatcg ctagcaccag gtataaccaa cgaaacattg ccatagtttg ctttgccccc
     3541 ttcacagggg gctttttttg aaaaacggct gcggaattag accagttatc tcccgaggaa
     3601 ggaaatttcc gcagcgcgtg ttctacttct gcccgtaata agcgttagga ccgtgtttac
     3661 gcatgaagtg tttattcatg aggtagctgt cgatgtgatt cagttgtggg ttaatgccgc
     3721 gggcaatcca cgccattttc gccacttctt ccatcaccac cgcgttatgc accgcatcgt
     3781 gagcatcttt cccccaggcg aacggcccgt gctgatacac cacaattccc ggcgtatgca
     3841 gcggctcggc gttgcccagc gtttcgataa tcactttgcc ggtgttcagt tcatactcgc
     3901 cctgcacctc ttcttcgctt aacccgcgcg tacacggaat gtcgccaaag aagtagtcgg
     3961 cgtgcgtggt gcctaacgcc gggatcgcca gccccgcctg cgcccatgcg gtggcatgag
     4021 tggagtgggt atggacaatg ccgccaagcg acgggtaacg acggtagagt tcaagatgcg
     4081 tcgcggtgtc ggaagacgga cgatattccc cttccaccac cttaccgctc atatcgacca
     4141 ccaccatatc gtctgctttc atggtttcgt aggcgacgcc gctgggcttg atcaccacca
     4201 gcccgcgttc gcggtcgata gcgctgacgt tgccccaggt aaaggtcacc agcccatagc
     4261 gcggcagatc catgttggct tcaaatacct gctgttttag cttttgcatt atgccgcctc
     4321 caccatgccc gctttcgcca tgcgcgcttt cacccaatca cgcgctttcg ccacttctgc
     4381 cgccgggtct tccgccgttt cgctccacat ctcaatcagg tacggcccgc aatagccact
     4441 ctgtttgagc gtttcgaaac aacgttcgaa atccactaca ccttcgccaa acggcacgtt
     4501 tttgaagacg ccaggtttgg tgtctttcac atgtaccgcg acgatatgcc cgattccggc
     4561 ctgcaactcc atctgcacat cgttgtccca cgccgacagg ttgccgatat ccgggtagag
     4621 ctggaaccac gggttgttga gatagtgcgc gtaacccagc gccttgctga tggagttcat
     4681 caacggataa tccatgatct ccatcgccag cgtcacctgc gcgcggctcg ccatctcaac
     4741 gctctctttc aggccgtcac ggaaacgacg acgcgtttcg ttattggctt cctgatagta
     4801 aacgtcatag cccgccagct ggatcacgcg aatgccgacg tcctgggcga actggatagc
     4861 tttacgcata atctccagcc cctgcgcccg caccgcgtca tcttcactgc ccagcgggaa
     4921 acgacgatga gcagaaaggc acatggacgg cacgcgcacg ccggtttcaa caatcgcatt
     4981 caccagcgcc agacgctgct cgcggctcca gtcgaggcgc gacaggcgct cgtccgtttc
     5041 atctaccgac atctcgacaa aatcgaagcc caacgttttt gccagttgca ggcgttccag
     5101 ccagcactcc ccggcgggga gcgctttttc atagatgcca agcgggattt gtttggacaa
     5161 catatccgct ccttagcccc acagttcggc gatggaacgt ttgaactgac gtgcagcttc
     5221 aaccggagaa gcggcatcac ggatgctgcg accagcgata aacacatgaa tcggaatacc
     5281 tttgaacagc ggcagatctt ccagcgccag gccaccggtg acggtgactt tgaagcccat
     5341 atcggaaaga cgtttgatcg cggtaatgtc cgcttcgccc cacgctacgc ctgcggcctg
     5401 cgcgtcacgg ctgcggtgat aaaccacctg ctgaatgcct gcgtcacgcc actgctgcgc
     5461 ctgttcccag gtccagtagc cggtcaattc gatctgcacg tcgccgttaa actcttttgc
     5521 tacgtccagc gcgcctttgg cggtgttgat atccgcacag caaattaccg tcacccagtc
     5581 agcgttggct tcgaagcaca tacgcgaaag gattttgccc gcatcagcaa ttttagcgtc
     5641 tgccagtacg attttgtgcg ggtagagcgc tttcaggtca cgaaccgcac gcacgccttc
     5701 gcccacgcac agaatggtac ccacttcgat aatgtcgact tcttcggcaa tcaggcgagt
     5761 ggtttcgtag gcgctatcca tagtctggtt gtccagcgcg acttgcaaca tcggtaatga
     5821 catcttcaat tccttcttaa gctgccgcgt tggtgcggtc aatgagatcc agtacttcct
     5881 gctcggtacg gcaggcacgt aaacggtcga aattttcttc atcttcaaac aggttgacga
     5941 tctgcatgat gcccacttcc tggtgcgtgt tggcatcgac cgccgccatg gtgatgagga
     6001 tatccaccgg gtcgttatct tcgtggttga actccagcgg ctttttcagc gtcaccagcg
     6061 caaaaccggt tttcttaacg ccctcttccg ggcgaccgtg cggcattgcc aggcctggtg
     6121 cgagcacaaa gtacggaccg tgctgttcaa ccgcatccag aatcgcctgg tagtaacgcg
     6181 gctcgacaac atccgccgcc accagcaggt caacgccgat ttttactgcg tcctgccatg
     6241 tctcagcttc agcctgcagg cggatggatt tattctccgc cagcgaatca cgtaatttca
     6301 tggcgcgtcc ttacttcaca tcctgcggga aatgcgcttt gatcacttcc agcagtttcg
     6361 ggccaaagtc ggcaggagag agcatgttgc gcacgccaac cacgtatttg ttgccggtca
     6421 cggtgatttc gcccgcaatg tgcgtagaag cgatgatgat atccgcgccg ctcaactcgc
     6481 ttttgtattc gccaaccgcg cagctattta ccgtgtggtc aatgtttgat tgggttaaaa
     6541 actggtccac tttcatcttc atgatcatgg aactgccttg tccgttgcca cacacagcca
     6601 gaatacgtac ggtcataatc aaaactcctt attaagcaga ctgttctgcc agttgttttt
     6661 ctgcatcttc ttctgcgcgc agtgcgcggc cagcgaagaa catataagcc agtgcaatta
     6721 caatgatgac ggccataaag gcgataccga tggagaagaa gccctgcatc atcggcggtg
     6781 ccagaatcga ccagtccgcc atgcccatcc aggcgctcat accggtgagt ttcaccgccc
     6841 agacgcagcc aaagatttca atcatcccca tcaccagaca tatcttcagc gccgcacgcc
     6901 agccgccgaa gtggttagcg aacacgccga tggtggcgtt ggagaagaac atcgggataa
     6961 agccgggaat aatcaggatc gaggaaccgc aggcgaccag gatgccaacc gcaatcagct
     7021 gaccgatggt gccccacata aagccccaaa ccacggcgtt cggcgcgaag ctatagatag
     7081 ccgcacagtc aatcgccaga accgcaccag ggatcaggcg ctgggagata ccgttaaatg
     7141 cttcagagag ttccgccaca aacatgcgca cgccctgagt gatgatgaag atcgccaccg
     7201 cgaaggagaa accagtttgc aggatgtaca ccgtccagtt cactttgcct gccatcgcct
     7261 gcacggtgtc gataccgaag gagagcagaa tggcaccaaa gaagatggtc atcacaatcg
     7321 ccgtggagac gatgttgtcg tggaaaatat tcagccagcc tggcagtttg aggtcttcaa
     7381 cgctttcctc ttttttgccg aggaacggcg caactttata ggcaatccat gatgcaaact
     7441 gttgctggtg accgatggag aaaccgcagc cgtccgtcac ttcctgagtc ggcttgtaca
     7501 tcatgttgga ggtaatgccc cagtagagcg aaaccagaat cgccgtgcag ataatggttg
     7561 tccacatgga gtagccgaaa ataaacaacg taacggcaat cagccccgcc tgctggaaca
     7621 tgatatgacc agtcagcatg atggtacgaa taccggtaat gcgacgcagc agcacgtaac
     7681 agatgttcag cgccagcgct aacaacaccg cgtaacctac ccagctatag gcatcgccca
     7741 tgcggtcgat ggttgccatc atcgacgcgt aagtatcgga aattgcgccg ttaatgccgt
     7801 agacttcgga cattttcgcc accaccggtt tgaaggtgct ggtgaggatg ccggaccctg
     7861 cctgcaacaa catgaaacca attatggttt taatcgtgcc tttaataata acgctgacac
     7921 ttttgcgcag taggatgtag cccagacagg tcacaatacc cagcaacaac ggggcattgg
     7981 tcatgacctg gttaaaaaac acggtaaaga tgttgtagag gatctccata acgatctcct
     8041 gaagaagagg taaccgggta ttccacacac ccggaatgtt gtgcactcac tctaattttc
     8101 aaaagtaatc acaacaagat tatttatgat taaatgtgac gcgacccgca aataattcca
     8161 catggataga atgggattca cacaacgaaa caactattca tttaaattca tttagttaat
     8221 tgaattacac attccattaa atctttccac actcaaaatc aacaaccact tccctggcaa
     8281 aaagtcaaaa aatggtcatt gccttcctga aaaaatagtg tcaggattaa tcaaaaccaa
     8341 tcacatattg attcgaattg aactatcatg aaggtaaatg gcgatgagta aagtgaaaag
     8401 tatcacccga gaatcctgga tcctgagcac tttcccggag tggggtagct ggctgaatga
     8461 agaaattgaa caagagcagg tcgcccctgg cacatttgcg atgtggtggc taggctgcac
     8521 cgggatctgg ttgaaatcag aaggtggcac caacgtttgc gttgatttct ggtgcggcac
     8581 tggcaaacaa agtcacggta acccgttaat gaaacagggt caccagatgc agcgcatggc
     8641 tggcgtgaaa aaactgcagc caaacctgcg caccaccccg tttgttcttg atccgtttgc
     8701 gattcgccag atcgacgcgg tactggcgac tcacgatcac aacgatcata tcgacgttaa
     8761 cgttgctgct gccgtgatgc agaactgtgc tgatgacgta ccgttcattg gaccgaaaac
     8821 ctgtgtggat ttgtggattg gctggggcgt accgaaagag cgttgcatcg tggtcaaacc
     8881 gggcgatgta gtaaaagtga aagacattga aattcatgcg cttgacgctt tcgaccgtac
     8941 tgcactgatc accctgcctg ccgatcaaaa agcggctggc gtactgccag atggcatgga
     9001 cgatcgcgca gtaaactacc tgttcaaaac ccctggcggt tccctatatc acagtggcga
     9061 ttcccactac tccaactatt atgcgaagca cggtaacgaa catcagatcg atgtggcgtt
     9121 aggatcgtat ggtgagaacc cgcgcggtat taccgacaaa atgaccagcg ccgatatgct
     9181 gcgtatgggt gaagccctga atgcgaaagt agtgatcccg ttccaccacg atatctggtc
     9241 aaacttccag gccgatccgc aagagatccg cgtgctgtgg gagatgaaaa aagatcgcct
     9301 gaagtatggc ttcaagccgt ttatctggca ggttggcggc aaatttacct ggccgctgga
     9361 taaagacaac ttcgagtacc actatccgcg cggtttcgat gattgcttca ctattgaacc
     9421 ggatctgccg ttcaagtcat tcctgtaatc tgtcgtttat accggatagc gtggagaagt
     9481 tcgcgctatc cggcgtagta tttcctggct atttcaaata tcatctaaaa aaatcaaatt
     9541 ttatcggaat agctcatgac tgaagcacaa agacatcaaa tcctcctgga aatgctcgca
     9601 caattgggct ttgtgaccgt tgagaaagtc gttgagcgtc tgggaatttc gcctgccact
     9661 gcgcgacgcg atatcaataa acttgacgaa agcggcaaac tgaaaaaggt gcgcaatggc
     9721 gcagaagcta ttacccagca gcgcccgcgc tggacgccga tgaatctgca tcaggcgcag
     9781 aatcacgatg aaaaagtacg tatcgctaaa gcagcctcgc agctggttaa tccgggcgaa
     9841 agcgtagtca tcaactgcgg ctccaccgcg tttctgcttg ggcgggaaat gtgtggcaag
     9901 ccagtgcaaa tcatcactaa ttatctaccg ctggcaaatt acctgatcga tcaagaacat
     9961 gacagcgtga tcattatggg tggacagtac aacaaaagtc agtccatcac tttaagcccg
    10021 cagggcagcg aaaacagtct ctatgccggg cactggatgt ttaccagcgg aaaagggctg
    10081 accgcagaag ggttgtataa aaccgatatg ctgacagcaa tggcagagca gaagatgctg
    10141 agcgtggtag ggaaactggt ggtactggtt gatagcagta agattggcga acgcgcggga
    10201 atgcttttta gccgtgccga tcaaatcgat atgctgatca ccggcaaaaa tgctaacccg
    10261 gaaatcctgc aacaactgga agcgcaaggg gtcagcattc tgcgtgttta aagatgctgg
    10321 cggaaaaatg tcacggcagc atctaacgcc tcaggcgtaa tgcggtgacg cacgcctggc
    10381 tgccatgaac aggttagcag tttatcccgt cccgtctcgc ttaaggcctg ctgcaaacgt
    10441 agtgattcgt cggcaggcac aacgtcatcg tcgaggccat gccacagcag tagaggtctg
    10501 tccccaagtt gttccaggtg gtttgtcgct tcccactctg ccagcggcgc gataatgtta
    10561 ttgaattcat tctgctgtgc tgccgtttca ggtatcagcg gtggaaacag tgaacgggcg
    10621 agcgatgtaa aatagcccga tcccatcatg ctggcggtac atttcaccgt ggggtggcga
    10681 gcggtaattc ccagtgccgt catcgcgccc atcgacgcgc caccgactgc cagacggtca
    10741 tcaagcagcc agttttcttc ggctattgcc gcacgtaaag tagtgaattc ctgcatactt
    10801 tgtagcaaga tttgccagaa ttgatttaac cgccgcgctt cgtcgctact aaaacggcta
    10861 ccgtgatcgg gcgcatccgg catgatcacc cgcaaaccag cctgcgccag cgcaacggca
    10921 aaatagctat acaccaggct ggatgaagta aagccgtgat aaaaaattac gcacggtaac
    10981 ggggtatctt tttgcccgac aggataagca tgaagaacgg gaatatctgc cagctcgcgt
    11041 gattctattt caatcatctg tctctccttt ttgtctggta tgacatgaag aaatccttag
    11101 cacaagtttc tgctggcggt attagttcat aatgttgaga tgtgggttac gctttcgtca
    11161 cgttttcatt cgaaaatcgc gtaccaggta acaattcggg aacattcccc caaagttaaa
    11221 ttaataaggc actacactat ggttagcagg aaacgtaata gcgttattta ccggtttgcc
    11281 agtttattat tggtgttgat gttaagtgcc tgtagcgcac tgcaaggtac gccacagcca
    11341 gcaccgccag taacgaacca tccgcaagag attcgccgcg accagacgca agggttacag
    11401 cgaataggta gcgtaagcac gatggttcgg ggttctccgg atgacgcatt agcagaaatc
    11461 agagcaaaag ctgtcgctgc aaaagctgat tattacgttg tcgtcatggt ggacgaaacc
    11521 atcgtgacag gacagtggta ttcacaagcc attttgtacc gtaaataaat cagacaactt
    11581 acatagtcag ctttacattg ctttgcgtcc ctatgcgttt acctgtgcac aataaattac
    11641 cggctggaag cctgggcgtt tattaccgcg atggaatgcc ctgcaacatg tggggaaacg
    11701 aaaaatggag ctgacgatga aacaattact tgcctcaccc tcgctgcaat tagtgactta
    11761 tcctgcgagc gccacggcac agtctgcaga attcgccagt gctgactgcg tgacgggcct
    11821 gaatgaaata ggtcagatct cagttagcaa tatttcaggg gatccgcagg acgtggaacg
    11881 tatcgtggcg ttaaaggccg atgaacaagg tgcgtcatgg taccgcatta ttaccatgta
    11941 cgaagaccag cagcctgata actggcgcgt acaggcgata ctgtatgcat gagtgtcatc
    12001 tacagcaaat gaacaatgcc ggatgcggcg cgagcgcctt gtccggccta caattttcac
    12061 atatttcatt gagttaatcg aaaccagcgt cgcatcagtc gtatacgctt acacacacac
    12121 tccccccgtc gcccgcagca ataaatcatt ctggatctgc tctgacaacc gtacgccgcc
    12181 gcgcgtatct aacatcacct gacaccacgc ctgtgccatt ggcggagagg catatttcaa
    12241 catttgcgca ccgcagccca gcaggaatag ctgatgagta atctctcgcc ccaattcttc
    12301 agctggcttg cgcagctgct gctgtaaacg acgaaccgcg cgatcaaaat agcgatcctg
    12361 ccctttcact tccacaaacg cttccgacaa taagtcgtat acgcccgctt gcttattgag
    12421 aacgcgcagc acatccaggc acataatatt gccggaacct tcccaaatac tgtttaccgg
    12481 catctcccgg taaagccgcg gcagctcgct ctcctcgcaa taaccaatgc cgcccagcac
    12541 ctccatcgct tcggccacaa acggcatacc tcttttgcag atcacaaatt ttgccgcagg
    12601 cgtaaataaa cgcgcccaca gggcttcttt ggcatcggca cgccggtccc acgctcgcgc
    12661 aagacgaaac agcaacgccg tttgcccttc aagctgaagt gccatgcgac ttaagacatg
    12721 acgcataagg ggctgttgga tcaatggatt accaaaaaca tggcgttgat gtgcatgata
    12781 aatcgccagc gaaaatgcac ggcgcatcat ggcatggcta cccagggcgc aatcaaaacg
    12841 cgtcatccca cccattttca ggatcagacg aattccttcc ccttcctgcc ccaacaacca
    12901 gccaatggca tcctgaaact ccacttcgca gctggcgtta gagcgattac ccagcttatc
    12961 tttcagccgc tcgaggcgaa tcgcgttgcg ttgcccgtca ggcaaaaagc gcggcacaaa
    13021 aaagcaggac aaaccgcccg tagtctgcgc cagcaccaga tgcgcatcgc tttgcggcac
    13081 cgagaaaaac catttatgcc ccaccagccg ataagagcca tcttccaggc gctctgcgcg
    13141 ggtggtgttg ctcatgacat cggaaccgcc ctgcttttcc gtcattccca tgccaatcaa
    13201 caaaccgcgt ttttgcccac ctggcaataa gtgagaatcg tagcgatcgc tcagcagcgg
    13261 cgtggtccag tcctgaaacg gcgcgggtaa catctgtaac agcaatggcg tggcggcaaa
    13321 ggtcatggtt atcggacata acgaccctgc ctcaacctgt gcgtgcaaca taaaacgcgc
    13381 cgcgcgcgcc acaaatgcgc cggagcgagc gtcttcttcc caggcaagat tgtgcacccg
    13441 attggtacat agcgcctgca tcagcaggtg ccaggcgggg tgaaaacgca catcgtccag
    13501 acggcgtcct tgcgcatcgt agcgcaataa ttcaggcgga ttcacattcg ccagccgccc
    13561 cagttcaagg gattcagccg ttcctaactg ctgaccaata ctggctagaa aatcgctatc
    13621 ccagccggca ccttcacgcg ttaccgcttc gcagagcgcg ccatcagaca ggtataagtt
    13681 gctgttattt aatggtatag gttgattaaa aacggtgtga gtttgccagt gcactgtgtc
    13741 tccctccatc aatggcagtc accattagta tggtcactgc catggattcc tgacagaaag
    13801 agattgctaa aacattctta gccagcaatg taatgcggaa cgaaacgaga ggtgtctttg
    13861 gtgatcagcg tgttatcttc acggatcccc attccgcacg cttcatcatc gacaatccag
    13921 ctaccgatga gtgtgtagct atcgccaaat cgcggcagag gttgaaacgc ctggtagatc
    13981 atcggttcat cggcgtaatc accatcagcg tggtcaacga cgttattctg accgtcgaaa
    14041 atggtgacgt ttccgccttc gcgcgagtag atcggtttac gcacatagct ttcgccagcg
    14101 gcaatctgcg atttttcacc ctcaaaccag gatgcaagaa ggttaggatg accagggaag
    14161 aagcgccaaa gcagcggcat tagcccttta ttactcaaga tacttttcca taacggctcc
    14221 acccattgct cgcgacgctt gcacagcagc ggaccgttat cgtcacgcat catccactcc
    14281 agcggataca gcttaaatgc acgctggatg acattatcat caagatcggt cagtacaccg
    14341 ccgacgccca aaccgagatc ttcaatgtag ataaaccgcg actcctgccc tgcctgctga
    14401 gcgcagtcct gcaagtacag cacggtagtc ctgtcttcat cggtgtcctg acagcagcag
    14461 aaataaaacg gctcccggct ataaagctcg ctaaagcgtg aaatcaggcg ttcctgaatg
    14521 gcattgtact gatcggcatc acgcggaata atgccgctgc gccgggcatc ctccagccac
    14581 aaccactgga aataagccga ctcatacaac gaggtcggcg tatcggcgtt gtactccagc
    14641 agcttcaccg gcgcattgcc acaccaggca aaatccatcc ggccatacag cgaaggatcg
    14701 cgtgcgcgcc agctttcggc gatcacatcc cagtacaacg gcgggatcgc cagttgcgtc
    14761 aggatctctt cgtctttaac cgcgcgatcc accacctcaa ggcacatctg atgcagttcc
    14821 gcagtcggtt tttcgatctg ctcttcaatc tggcgcagag taaaacggta agcccgactc
    14881 tcgtcccaat agatttcatt gtcgatgata tgaaagtcga aaccgttgtt agcggcgatc
    14941 tggtccagat cccgtcgcac aggaacgttg tgtctcagca tgattagcct ccccaatgcc
    15001 cacgggcgct ggaagaacga ccatagccgc cgcgcgaaac ggttgacgct tttttggtgg
    15061 taaagccgcc tgaagagtaa gactcttttt tgccagaacc ggagcgccag gagtaatcac
    15121 cggaagtgtt gcgccagacg gggcgggaag caaatgagga tccgccgctg ttatagacga
    15181 acggctcatc gcgatcttta cgaataactc ggcttaacaa aaatccagaa acgaccggga
    15241 tccaactctg ttcaacattg tcgtagtagc aattttcgta cttagactga caattctgct
    15301 gagtcatatt cttcggaaca tcggcataaa atgccgcttt ggcgttgttc cagccacgcg
    15361 cgcaaatgtc ggcattatta ccgtcatcaa tacaatcctg cactgtcgaa taaaacgttc
    15421 cgtcgccgtc gttatcgaca tcgccacttt cactccatcc tttgactatt aaaaaaacgg
    15481 caccgcccat tagcaccaac gttaagtatt tttgtgcgta gcgattgcgg cgcggctcaa
    15541 aaggattatg cggtcttcca atgcgactga tcgctccgtg accaatttta ctgttatttc
    15601 ttgatttgcg ttttctggcc atgatcgtcc attaccatgt catacaagct gcgttaaaaa
    15661 taccaccagc cagtgcggcg gttcccataa acattcctgc tgcggtattg tgattaataa
    15721 ttttttcgct taatgcgggc atataaagtc tgacaccagc aaaaacgagt aactgaatca
    15781 ccagtgcaat cccgccccag gcgaaatagt ctggaatact caccgcattg attgccgcac
    15841 tggataaggg gatcacgtaa cccaacaatg taccgctgaa tgccagtgac gccgcggtat
    15901 tattgttttt gattaattgc cattcgttgt gcggtgtaat tttggagtag ataaacagaa
    15961 aaataatcac catagccacg ccaataaaaa aataggcact aaaggcaaga agtgaatcca
    16021 gtatatgcat tgtaaacgtc cttgtaatta accaataata tgaagtgatg tcagcgggat
    16081 atcgacaccc aacgcgcgcg aaaataacca ctctggctcg ccgagatcgt taaaagattc
    16141 ctcaccattt aacagcaaat attcgtaagt atcttcggtt acttggcgtt ggtagcccat
    16201 ggtaaaatta tggacctccc atttggcatg gttttgattt tctacttttt ccagcatgta
    16261 gacgggttcg atatttcccg gttcttcgct gttaaaaaat cgctgccagc gtttttcctg
    16321 ccagtttaag atcattgccc ccatcacttt ggcgttgatc gcctcgcgcc agtgactctc
    16381 tttactgata ccgtagctct cttcatagac aaagagcttg atatcatcaa tgtcatcaat
    16441 atcttcgccg ccggtggtat tgatttgcag aaattcatcg cctgaagtgt agtagcggaa
    16501 aatctgacta ccgccgccca gatcgatgtg gctgacagcg gctaccgtaa attcctcacc
    16561 cggcagcgca atcagcaatt catcttccag caaacgaaac gctaacgtgt cgagcgtaaa
    16621 gccactattg agatgaagtc ccagcggacc gcgagcgata gcaggcttat tatccttgcc
    16681 aaacagacgc tggaaaaaac cagacatatt ttatccttaa aatagttgcc agccttttag
    16741 cgaggctggc aaaaaaatta ctcgccctgt tggcgttgca gtcttgctaa tacatcctgg
    16801 gcgctacttt tattgctacc gccaattccg gcttccgcca gcttttcatc aagatcgcga
    16861 ccgtctgcga ctttttccaa ctgtgcagca gcatccaggc gagcctgacg ttcggcctga
    16921 cgcgtttgca ggcgttttaa ggattctgcc gctgtcgaaa cgctggaaga tgcgccaacg
    16981 gtagaggttg ttacagcctg ttgtgcacgc tgcatggctt cagtggcttt aacgacttcc
    17041 atttgctgct caaactgagc gatacgctgc gctgtcgctg taactgcttt ttccacgcca
    17101 tcacgggaga cttccagatt cgacaacact tgctcttcag cggtaatgag attctcaagg
    17161 cgcgcgattt cttcagcaac ttcgttaatc aacgacggat taacgttctt gcttaacgct
    17221 tccagcgcac gggcttccag actggctttg cgctcacgta aatctttcag cttatcgtga
    17281 cttaatttca cccgcgccag cagatcaacg cgagattttc cggctttatc gagttcagct
    17341 ttagcgtcac gaatatgctg ttccagcatg cgcacgccct gggtttcttc aatggattct
    17401 tccgcctgga agataaacga tttccccagc gtaaataaac tttttaaaat tcccataaat
    17461 acccgtcctt gtaattagtg tgaatattct tctgtaattt cagccagatc caatgcgtta
    17521 tctaccagcg aggttatctc aagcaggata tcttcaagag aagatttaag cgataacgcc
    17581 ccgaaaacaa tgtaatactc ttcctgttgc acgctggaga ttcctaccga tgacaacggc
    17641 atcatcttct gatttcttaa taagaaggta ttaaattcat ctggattgct gatactactt
    17701 accggacaaa taaaggtttc gataatcatt tggcgggaag taaaaagaat attgatttgc
    17761 aaatcgccat aatcattcat tttaataatt aatgcgctct cgctatttgt cacatcaata
    17821 ttttgttcag gtacagtttg cagcgccgtc gccagcgcca acggattcca tgtcatatat
    17881 tattccatat agatttagtt taaatattaa taaaatgaat atttgcagta cgtaattatc
    17941 ttaccagcta tagacaaaaa aaaaccatcc aaatctggat ggcttttcat aattcagacg
    18001 aactagctgc gctgacgaac cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc
    18061 agggaagaaa cacttcctgc cattgggatg ctgatcaact catcgcaatg ttcacgggtc
    18121 aggcgacgca taccttcacc ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg
    18181 ctctgataga gtgtatgatc cgcctcgcct gccgtaccga cgatccagat attctcttcc
    18241 tgcaacatac gcatggtgcg cgcaaggtta gtcacccgaa tcagtggaac gctttctgcc
    18301 gcgccgcagg ctactttttt cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca
    18361 atcaccgcgt gaacgccagc agcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga
    18421 tcggttacac cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat cagatccggc
    18481 agatcgtttt cctgatactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg
    18541 gcaccgtcgc ttttctcgtc gagatattgg cggtttgcca actggataac cacgccctga
    18601 gactcaaggg cgtgaatcag cggtaacaga cgtttatctt cacggccttt taaaataaag
    18661 acttcctgaa aacgttcagg ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa
    18721 atcatttcgc tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc
    18781 ggcctacatg atctctgcaa tatattgaat ttgcgtgatt atgtaggccg gataaggcgt
    18841 tcacgccgca tccggcatga acaaagcgca ttttgtcagc aatctaaccc tcttctttta
    18901 aagagggtat tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg
    18961 cggctatttt ctgcgttttc gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg
    19021 ctttcggctt cgtttttttc tcaccgcgga aggcgctgtc tggctcaaag tttacctttt
    19081 taccgacctg acgacgcttg ccgccttttt tgcctgcatc gccttttttc gctttctcgc
    19141 gcgccgtttt accgacgttg cgcggtgcgc gttcactgga gatcagacta aagtcgattt
    19201 tgcgctcgtc catattaacc gcttcgacgc gaacttccac gcgatcgccc aggcgataag
    19261 tctggccgct ggattccccc atcaggcgtt gccctacctg gtcaaagcga tagtagtcat
    19321 tgtccagcga agagacatgg accagaccat caatgaacaa gtcgtccaga cggacgaaga
    19381 agccaaagcc agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca
    19441 tgaagtcaca cttcagccag tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg
    19501 ccatcgaaca gtgctgaccc agttgcaaca tctcttccat cgaataatgg tagccgccgg
    19561 tttcagtggt gttgccctga tgcccctgct ctttcgccag cagatactta atggcgcggt
    19621 gcagcgtcag gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg
    19681 ccaggccaaa gtgaccacgg ttttcaggat cgtaaatcgc ctgtttcatc gagcgcagca
    19741 gcatggtttg cagcatttct gcatctggac gatcggcaac cgactccagc agctccgcgt
    19801 agtcacgcgg ttccggctta ttaccgcccg gcagttccag ccccagctcc gccagaacag
    19861 aacggaaaga ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt
    19921 ctttggcttt ctcaacaaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa
    19981 tcaacttgtg tgcgtcgtta cgctgagtct gttcgatacg ttcaatacgg cgttcagcgt
    20041 tgaaaatgaa cttcgcttct tcgctctcaa atgagatccc gccgcgttct tcacgggctt
    20101 tatccagcac tttatagagg ttatgcaact cttcgagatg cttaaccagt ggggcgtact
    20161 gctcgcgcag atcctgatcg ccctgcagaa tatgccagac tttggtgtag gtcagacgcg
    20221 cgtgagagct catcaccgct tcgtagaatt tgtagccagt caggcggcct ttcgacgaaa
    20281 ccgtcatctc gcacaccata cacaggcggt ctacctgcgg gttgagcgag cacaggccgt
    20341 tagagagcac ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtaccac
    20401 ggttacgcgc ttctctgtcc agcggcgttg gcggacgcac atagtagctg acgtcggcaa
    20461 tcgcgaccca taaacgccag ccgccgccgc gttttttctc gcagtaaacc gcatcgtcaa
    20521 agtcacgggc gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc
    20581 ccgcttttgc ttcttccggc acttcttctt tcagcccggc aacctgttgc tcaacagcct
    20641 gcggccagat gtacggaatt tcatgggtac gcagagcgat atcaaccgcc atgccggtgc
    20701 ccatattatc gccaaggact tcgacgattt tacccaccgc tttggtgcgg cgagtcggac
    20761 gctgagtcag ttcgactacg accacaaagc ccatccgcgc gcccatgatc tgatcgggcg
    20821 ggattaagat atcgaagctc agacggctat cgtcaggaac cacaaagccg acgcccgctt
    20881 cggtaaagta gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg
    20941 cttcacgacg acctttacgg tcagcgccca gcggctgagc cagcacctga tcgccatgaa
    21001 tgcaggtttt catctgctcg ctggagagat acaaatcatc tttacgccct tcaacccgca
    21061 gaaagccgta gccatcacgg tggccaataa cggtaccttt caccaggtcg aggcgttccg
    21121 gcagcgcata gcactgacga cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca
    21181 ggcggcgacg caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct
    21241 catcacggct ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag
    21301 ggatgggatt cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg
    21361 ttcctccgtt gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag
    21421 cggttgattc tcttcaacca aatcggcaag cgtgtagtta tccagttccg taagaaaact
    21481 ttgcacggcc ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc
    21541 actgctgcaa ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat
    21601 cgcactcgcc gatttaccca ggcgaatgcc gccatttttt ccacgaacag cagtcacgta
    21661 gccggcacga ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac
    21721 gtcagtcact tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag
    21781 cgcacgtaat ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc
    21841 gggaaaaaat acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt
    21901 cagttcgggg aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt aatggcttaa
    21961 ccgacacagc aatgtatcac actgttagcc cggcaggcaa aatatctgcc aggcgtacca
    22021 gaattacgcg tcgaacggat cgcgcagaat catggtttca gtacgatccg gaccggtaga
    22081 gatgatatcg atcggcacac cagtcagctc ttcaatacgc ttgatgtagt tcagcgccgc
    22141 ctgcggcaga ccgctacgat ctttcacgcc gaaggtggat tcagaccagc ccggcatggt
    22201 ttcgtaaatc ggctctacac ctttccagtc gtcagctgcc agcggagtgg tagtcacttc
    22261 gcgaccatcc ggcatacggt aagccacgca gagtttaacc tctttcaggc catccagaac
    22321 gtccagttta gtcaggcaga agccagacag ggagttcagc tgtaccgcac gacgaacggc
    22381 aacggtgtcc agccagccgg tacgacgacg acgccccgta gttgcgccga attcgttacc
    22441 ctgcttgcag aggaactcgc cagtttcatc aaacagttcg gtcgggaacg gacctgcacc
    22501 tacacgagtg gagtaagctt tgaggatacc cagaacgtaa tcaacataac gcgggcccag
    22561 gccggaaccg gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggataagt
    22621 accgtggtcg atatccagca gcgtaccctg cgcaccttca aacatgacga aatcgccacg
    22681 ctgacgcgcc tggtcgagca ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc
    22741 aacagccatc gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt
    22801 aaccaactgg aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt
    22861 gtcgaaaagg tcgccaacac gcagaccgcg acgtgctact ttatcttcat aagcaggccc
    22921 gataccacga ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc
    22981 cagcgcaacg tgataatcaa ggatcagcgg acatgcttca gacagcagca gacgctcacg
    23041 aacggggatg ccacggtctt ccagttcttt catctctttc atcagcgcgg ccggagacag
    23101 cacaacaccg ttaccgatga tgctggttac attctcgcgg agaatacctg atggaataag
    23161 atggagaacg gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg
    23221 gtagcgtaca acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc
    23281 gtcaccccat tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt
    23341 gcttaaaaat ggattctacc atcgcttttt cagagttaca gcactttttg cactcaaaaa
    23401 tgacctcgat cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca
    23461 caacgcccgc aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg
    23521 tcatcgcaga gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca
    23581 gtaccaaaac cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa
    23641 aaagaaaacc accgacatcc ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt
    23701 tatattaacg cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc
    23761 tcatgaccat cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga
    23821 tgaatgcgta gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttcgg
    23881 catcgccttc accacgcatg atgcggccct gacgctcagc ttctgccagc gttctggtca
    23941 cttcatagtc ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac
    24001 gacgcgctac cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg
    24061 tcggcaggtt gatctgcttg atacgcacat cgacaacttc aatacccagc gccgccatac
    24121 tgttcgggtt gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt
    24181 cggcaatggc gttatctgcc gccggggtag taacttcatc ttctgtaccc gcagaaccgg
    24241 agttcagcgc gtcacgtact tcgagggtca gacgaccacg ggaatcggtg acgatatctt
    24301 tcacgtccag gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca
    24361 cttccgcttg cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg aaatcgctga
    24421 tgcgccattt gatgtaagag tcgacgatca ggtctttctt ctctttggtc acaaagcggt
    24481 cggcctggtt gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg
    24541 gtatcttgaa atgcagacca ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta
    24601 ccttaccaaa acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt
    24661 aaagcactac cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc
    24721 ctgacgctgg tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg
    24781 actggtggac gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcagacg
    24841 cagcagattg ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat
    24901 ctggtctaac ggcagaacca tcaggttgcc acctttatcg ttaaccagca ctttgcgggt
    24961 gttacccaac actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc
    25021 ggctttatat tccggcagaa gtttagcaaa gcgcgccact tcaccctgag cttccaggat
    25081 ggtctgggcc ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg
    25141 cggctgaact tcgttggtat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg
    25201 cgcggcaatc gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa
    25261 gttgacgtcc agcagcgtga tacccatgtc atacggacga atcgtctctt ccagttcgcg
    25321 ctgagtatcg ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt
    25381 accgataact ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat ccgggctggt
    25441 cacgctatac agatattttt ccggattggt gacgcggtac tgcacgttca tctcaacgcg
    25501 cactacgttc tcgtccgacg tcagcatcac accagaagcg gccagttcac gcacggcttc
    25561 cacgttcacc ggtttgactt cgtcgataaa cgtcggtttc cagttcagac ccggctcaac
    25621 cagatggctg aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata
    25681 gaaaccactg gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacacgacc
    25741 gccaagctgc gggcgcgggc cttgcgatga actgccaccg ccagatccgg tgcctttacc
    25801 gccgcccaga ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg
    25861 cccttgatcg cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct
    25921 tccccacggg tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct
    25981 ccatatttgt tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc
    26041 ttcagacgct acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc
    26101 gacgccagtc aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc
    26161 actctttttc tattgcctga agctgataaa aacgacttct cagacgccct tcctgcggtg
    26221 gcagacgcaa tgtatgctgc gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa
    26281 gctgtggtat ccccgctccg gtctgtgcgg aaagccagac acggatcggt ttgttctctt
    26341 cgtcccgatc aatacgcggt tcgaaatctt ccagcatatc gatcttgttc atcaccagca
    26401 gggttgggat ctcgtgagcg tcgatctctt caagaaccgt attcaccgct tcgatgtttt
    26461 cttgtacacg cacatccgcc gcatcaatga cgtgcagcag taatgtggct tgccgcgtct
    26521 cttgtaacgt ggctttaaat gctgcaacca gatcgtgcgg caggtggcga ataaacccta
    26581 cggtatctgc caacacggtt tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt
    26641 cgagggtggc aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa
    26701 gggtagattt accggcgttg gtatatccca ccagcgaaac agtaggaacg tcggctttga
    26761 tacgcgattg ccgcccctgc tcacgctgct tttcaactct ttccaggcgc gactgtatct
    26821 gcacgatgcg attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac
    26881 gcaaacctat cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag
    26941 ccagatggcg cagctgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt
    27001 gggcgaaaat atctaaaata aggccggtgc ggtcgataac acgacactcg cacaaacgct
    27061 ccaggttacg ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcacccg
    27121 tcgctttgac agcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt
    27181 gcggcgcttt acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca
    27241 gagattcaaa ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca
    27301 gtaccgcctg ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc
    27361 agcggggaac gcaggatcgc tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg
    27421 tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac
    27481 tgctggtacc gccaccggcg ttgttactgt gatgagaaac cggacgagac gggacaacag
    27541 tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact
    27601 gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg
    27661 gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca
    27721 ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc
    27781 gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact
    27841 tcgtcacgcg cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc
    27901 agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt
    27961 tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga
    28021 atggaaggca agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca
    28081 aaacctgaag ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg
    28141 ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc
    28201 gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt
    28261 tgtggatcat ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga
    28321 tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg
    28381 tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg
    28441 cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg
    28501 cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc
    28561 ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa
    28621 ataagggcag aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct
    28681 aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc
    28741 ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa
    28801 cagaccaccc ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag
    28861 ggttatggcc tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca
    28921 cagcgcaata ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg
    28981 aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg
    29041 ctgtgcatct gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa
    29101 tgccgatttt tcttcggcag aaacttttag ccgcaacgga atcagcagcg gctgggcgca
    29161 aacgggcgct tcacccggcg tcaattgtgc ctgacgtagc caacgttctg ccactggcaa
    29221 ggataaaagt gaaatgttgc catcgcgctc cagcaacgca cagtcggaat ggacgatagt
    29281 cagtacccga ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc
    29341 ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg
    29401 tttctggtag cctggctgcg cattcggcca gggggcagcc ggacgactgc ctgaggcggg
    29461 cgcaggagag tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg
    29521 ccccgccgcc acgcggtttt ccggaatggg acgcggtgca ggttggggtt catcgtccag
    29581 cggtagcggc gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc
    29641 atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc
    29701 cacctgatgc gggtcgatct ccagatacaa cacaaacgcc ggttgttgat cggcccccag
    29761 tttgtcttcg caggcctggc ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc
    29821 attcacgtag caatactgaa tttccgccag tgcgggcgta gtgtgatttg gatcggccac
    29881 ccagccgcgc agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa
    29941 agcggtgcca caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg
    30001 gtactgacgc acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag
    30061 cgcaatgcgg cggatgattt catcaatatg gctaaattcg gttttctcgg tgcgcaggaa
    30121 tttgcgccgc gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg
    30181 atgcgccgcc ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc
    30241 ttcctgctgt tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc
    30301 cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat
    30361 tttactggtg gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc
    30421 gttatcacga atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt
    30481 cgcacctgca tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac
    30541 cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa
    30601 tcgtccttag tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt
    30661 tgccccctgc ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta
    30721 aatggcttct gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt
    30781 gttgctgata aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc
    30841 gagactcgcg tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat
    30901 catactggtc gctacatcat accctacccg ttgggaatga ccgaactgta aatccagcac
    30961 cgcctggctt aaataggggt cagactgact gttcgccagc acatcacccg ccccacccag
    31021 cagctccgac tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg
    31081 gttagagagc acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg
    31141 aatcgacacg aggaaattgg cgttttgctt acgtgccaca tcactgcgcc ccatcaccga
    31201 gataaagtaa tccccgtcac gggttaaaac gcccttaaac atcggatcgt cattgagcaa
    31261 agtacgcaat ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc
    31321 gatagcgcca gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc
    31381 agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt
    31441 agtgcggtta ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac
    31501 aaccgcaggc gtttcaacgc gtttcgcaac cacgggcggc ggaggaggag gtggcggcgc
    31561 atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc
    31621 ggttttaccg ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt
    31681 tccagagcga atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat
    31741 cacgcccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata
    31801 atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg
    31861 aatatcagag agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa
    31921 ccaatttctg atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc
    31981 cagcaacaat tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg
    32041 ataatcaatg tgtatttcga catccgggtc aggaagaaca ccagtacctt gttgtggcca
    32101 ctccaccagg cagatggcat cgttggcaaa ataatcgcga atccccataa actccagctc
    32161 ctcgggatcg gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata
    32221 gggttcgacc agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa
    32281 aaagccccgg ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt
    32341 tgcgccatcg caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg
    32401 gagcggaatt actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct
    32461 gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta
    32521 cgtcagctgc agcaccgtgc gcgacacagc ccgcacaggc ggcatcatac ggcgacagtt
    32581 tttgcccaag caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca
    32641 tgcctgcatt tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc
    32701 cggcaccttt cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta
    32761 agcggtcact ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat
    32821 gcggcgtgat cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat
    32881 cggcatccca caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct tttttccccc
    32941 actcttgctg gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg
    33001 taagagagtc catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg
    33061 caatgttttc actgcgggtc agtactcgga ctaaaccagc accagcacgt agcgccgctt
    33121 cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc accgataatt accagccgcc
    33181 cgtgatcgcc tttatgcgaa gtcgggcgac gcggttttag ccagtgagaa agttgttctg
    33241 ctgaaaaccg ctgaattttc gtctcctgac ctgccagcca actatccagc cccagtgagt
    33301 caaaatgcag ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg
    33361 caataaaagt gatggtgtga tcggcgttga tcaccgcgcc tggcgtagcg ccagtttcag
    33421 ccagcaggcc ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt
    33481 cgattaactg gctaatggat tcgcggggcg cttgccgcaa accggtaccg agcagcgcat
    33541 caactatcag atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg
    33601 cgtttaacca tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct
    33661 gggccaacaa cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat
    33721 cgccgccgtt attaccatga ccgcacagca ccagccagtg gcgggcgtca ggatacgccg
    33781 aacgacacac ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc
    33841 ccagcacatc tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt
    33901 gtggtatact tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc
    33961 tcaatcagtt agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag
    34021 gtattaccga taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac
    34081 aataccacgg cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgccctcatg
    34141 agttattgcc cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg
    34201 ccgcatttgc cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg
    34261 gccgtgacta tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc
    34321 aacattgtgt ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc
    34381 cgttagctga aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg
    34441 aggccggttc tttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc
    34501 aaccagtcga ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg
    34561 ccatcgtcga gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac
    34621 ttgaaggggc gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg
    34681 atgactgcca gcttatctgc ccgtggaatc gctattcaca actcaccaca gaagaggatt
    34741 tcagcccgcg taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag
    34801 agaagttttt aaaagtcacg gaaggatcgg cgattcgtcg tattggtcac ctgcgttggc
    34861 tgcgtaatat cgccgtagca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc
    34921 tggaaagtcg taaaggtgag cacccacttc tcgatgagca catagcgtgg gcgattgcgc
    34981 agcaaataga gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc
    35041 tggttcgggt gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa
    35101 taaaaataaa aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca
    35161 aaacggaatc ttaattttta acttatctat caataggtta aaaaaatact attcaccgtg
    35221 cgaagtttct ttgaaattaa gattacagcc tgaggctgtg gataactctg tttacaagag
    35281 tttgtcagag acaacgaaaa ccagcaataa cgcatcgcat cgctgtggat attttattga
    35341 gagaagaatt tggagcggga aacgagactc gaactcgcga ccccgacctt ggcaaggtcg
    35401 tgctctacca actgagctat tcccgcttgg gtggtctgtg ccttacagca ctttcaaatt
    35461 ttggagcggg aaacgagact cgaactcgcg accccgacct tggcaaggtc gtgctctacc
    35521 aactgagcta ttcccgcttg ggtggtctgt gctttgcagc acttttcaaa ttttggagcg
    35581 ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc
    35641 tattcccgcg tactacttaa ttttgctttc gtaattttcg catttctgcg tcgttacggg
    35701 aggcgcatta tacgagaaat ctttccctct gcaacccccc aaaaaagttt tttttgcgtt
    35761 tttatctcaa gtgctgaaat tatcagcaag gcgttcaatt tagcgtcaaa atcacgccgc
    35821 cgcaagtgga ttccggcgct tacgtgacca ggaaaaattt tacagcttga taaaatgctc
    35881 gcggtagtaa gccagctccg ccaccgattc acggatatca tccatcgcct gatgcgtccc
    35941 ctgcttggta aaaccatcca gaatttccgg cttccagcgg cgcgccagct ctttcagggt
    36001 gctgacatcg agataacggt agtggaagta ggcttccagc tccggcatgt atttaaacag
    36061 gaaacgacgg tcctgaccga tgctgttacc gcaaatcggc gattttcccg caggcaccca
    36121 ctgttttaaa aattcgagcg ttgccagttc agcttcccga tcgcccatcg tgctcgcttt
    36181 cacgcgctct accagcccgc tggcggtatg ggtgcgcacg ttccagtcat ccatcagcgc
    36241 cagctgttca tcagactggt gtactgcaat ggtcggccct tctgccagaa tattcaggtt
    36301 ggcatcggtc accagcgtgg caatctcaat aatgcgatcg cgctcgggat ccagaccggt
    36361 catctcaaga tcgatccaaa tcaggttgtt ttcattggca ctcatgctat tttccaccca
    36421 ttcacagtga ctatattgat tcacaattgc gtgtatcata gatgttttgc ccatcagggg
    36481 cgaccaggag tctgtacgat tgagtaaaaa taaactctcc aaaggccagc agcgccgcgt
    36541 gaacgccaat caccagcgtc gtcttaaaac gtctaaggag aagcccgact acgacgacaa
    36601 tctgtttggc gagcctgatg aaggtatcgt catcagccgc tttggtatgc acgctgatgt
    36661 ggaatccgcc gatggcgacg ttcaccgctg caatattcgc cgtaccatcc gttcgctggt
    36721 aaccggcgac cgcgtagtct ggcgtccggg taaaccggcg gcggaaggcg taaatgtcaa
    36781 agggatcgtg gaagcggtgc atgagcgtac ctcggtgttg acgcgtccgg atttctacga
    36841 cggcgtaaaa cctattgccg ccaacatcga ccagattgtc attgtctccg ccattttgcc
    36901 ggagctgtcg ctcaatatta tcgaccgtta cctggtggcc tgcgaaacct tgcagattga
    36961 gccgattatt gtgctcaaca agatagacct gctggacgac gaaggcatgg cgttcgtcaa
    37021 cgagcagatg gatatctacc gcaatatcgg ttatcgcgta ttgatggttt ccagccatac
    37081 tcaggatggg ctaaaaccgc tggaagaggc gttgaccggg cgcatcagca tttttgccgg
    37141 gcagtctggc gtcggcaaat ccagcctgct gaatgcgttg ctggggctgc aaaaagagat
    37201 cctgaccaac gatgtctctg acaactcggg gctcggtcag cacaccacga ccgccgctcg
    37261 gctgtatcac ttcccgcacg gcggtgatgt gattgattcc cctggtgtgc gtgagttcgg
    37321 cctctggcac ctggagccgg aacaaatcac tcagggcttt gtcgaattcc atgactattt
    37381 aggtctgtgt aaatatcgcg attgcaaaca cgataccgat ccgggctgcg ctatccggga
    37441 agcggttgag gaagggaaaa tcgcggaaac ccgtttcgaa aactatcacc gtattctgga
    37501 aagcatggcg caggtaaaaa cgcgtaaaaa cttttctgat acggatgact gacaattaag
    37561 ctaaccgtcg ttagaatcgt cccctttttt tcaggatccg gcatgttggc cggatcagga
    37621 acgacaaaac aatggcctgg aggctacctt gttaaattca tttaaacttt cgctacagta
    37681 cattctgccg aaactatggc ttactcgcct ggcgggttgg ggcgcaagca agcgggcagg
    37741 atggctgaca aaactggtta tcgatctgtt cgttaaatac tacaaggtcg acatgaaaga
    37801 ggcgcaaaag ccggacaccg ccagctaccg cacctttaac gaattctttg tccgtccgct
    37861 gcgtgacgaa gtacgcccaa tcgataccga tccgaatgtc ctggtcatgc ctgccgatgg
    37921 cgttatcagc cagttgggta aaatcgaaga agataaaatc ctgcaagcca aaggccacaa
    37981 ctacagcctc gaagccttgc tggcaggcaa ctatctgatg gcggacctgt tccgcaacgg
    38041 tacgtttgtg accacttacc tctccccgcg tgactaccac cgcgtacaca tgccgtgcaa
    38101 cggtattctg cgtgagatga tctacgtgcc gggcgatctc ttctccgtta accatctcac
    38161 cgctcagaac gtgccgaatc tgtttgcccg taacgaacgc gtgatttgcc ttttcgatac
    38221 cgaatttggc ccaatggcgc agattctggt cggagcgacg attgttggca gcattgagac
    38281 ggtctgggcg ggcaccatta cgccgccgcg cgaaggtatc atcaagcgct ggacctggcc
    38341 tgccggggaa aacgacggtt ctgtggcact gctgaaaggc caggaaatgg gtcgctttaa
    38401 actcggttcc accgttatca acctgtttgc accgggtaaa gtgaatctgg ttgagcaact
    38461 ggaaagcctg tctgtcacga aaattggcca gccgctggca gtatctaccg aaacctttgt
    38521 tacgccagac gctgaacctg ccccgcttcc tgctgaagag atcgaagcag aacacgacgc
    38581 cagcccattg gttgacgaca aaaaagacca ggtctaatcc atcaaaggaa acgctgacgt
    38641 gcgcctgatt atcacttttc tgatggcctg gtgcctcagt tggggggcgt acgccgcgac
    38701 ggcccccgat agcaaacaaa tcactcagga actggagcag gcaaaagcgg cgaaacccgc
    38761 acagccggaa gtcgtagagg cgctccagtc tgccttaaat gcgcttgagg aacgaaaagg
    38821 ttcccttgag cgcatcaaac aatatcagca agttatcgat aattatccga aactctccgc
    38881 tactctgcgc gcacaattaa acaacatgcg tgacgagccg cgcagcgtgt cgccgggaat
    38941 gtctaccgac gcgctgaatc aggaaattct ccaggtcagc agccagttgc tggataaaag
    39001 ccgtcaggcc cagcaagagc aggagcgcgc ccgcgagatt gccgattcgc tgaatcaact
    39061 gccgcaacag caaaccgacg cccgccgtca gttaaatgag atcgagcgcc gcctggggac
    39121 gcttactggc aatactccgc tcaatcaggc acaaaatttc gcgttgcagt ctgactctgc
    39181 acgtcttaag gcgctcgttg atgaactgga gctggcgcag ctgtctgcca ataaccgcca
    39241 ggaattagcg cgcttacgct cagagctggc ggaaaaagag agccagcaac tggatgcgta
    39301 tttgcaggcc ttgcgtaatc aattaaacag ccaacgtcag cttgaggcgg agcgggcgct
    39361 ggaaagtacc gaattactgg cagaaaacag tgccgatttg ccgaaagata tcgtcgcgca
    39421 attcaaaatt aaccgcgaac tatcggcggc tttgaatcaa caggcgcagc ggatggatct
    39481 cgttgcctcg caacagcgtc aggctgccag ccagacgtta caggtccggc aggcgttgaa
    39541 tacgctgcgt gaacagtcgc aatggctggg atcgtccaat ctgctcggcg aagcgctgcg
    39601 ggcgcaggtg gcacggctgc cagaaatgcc aaaaccacaa cagcttgata ccgaaatggc
    39661 gcagttgcgt gtgcaacggt tacgttatga ggatctgctt aataaacagc cgctgctacg
    39721 gcaaatccat caggccgacg gtcagccgct gaccgccgag caaaaccgta ttctggaagc
    39781 acaactgcgc actcagcgtg agttgctgaa ctcattgttg cagggtggcg acacgctact
    39841 attggaactg accaagctga aagtctccaa cgggcaactg gaggatgcgc tgaaagaggt
    39901 gaacgaagca acgcaccgct atctgttctg gacctctgac gtgcgcccga tgaccatcgc
    39961 ctggccgctg gaaatcgccc aggatctgcg tcgtctcatt tcgctggaca ccttcagtca
    40021 gttgggcaaa gccagtgtga tgatgctgac cagcaaagag acaattttgc cgctgtttgg
    40081 cgcgttgatt ctggtcggtt gcagtattta ctcgcgccgc tatttcaccc gttttcttga
    40141 acgttcggcg gcgaaagttg gcaaagtgac tcaggatcac ttctggctga cgttgcgcac
    40201 tcttttctgg tcgattctcg tcgcgtcacc gctgccggtg ctgtggatga cgctgggtta
    40261 cggcttgcgc gaggcgtggc cttatccgct ggcggtcgcg attggcgatg gcgtaacagc
    40321 caccgtgccg ctgctgtggg tagtgatgat ttgcgctact tttgcccgcc caaacggctt
    40381 gtttatcgct cattttggct ggccgcgcga acgggtttcc cgtgggatgc gctactacct
    40441 gatgagcatc gggcttattg tgccactgat tatggcgctg atgatgttcg ataacctcga
    40501 cgaccgcgag ttttccggtt cgctgggacg gctttgcttt atcctcattt gcggtgcgct
    40561 ggcggtggtc accctcagcc tgaaaaaggc cgggatcccg ctgtatctca acaaagaagg
    40621 cagcggcgac aacattacca accatatgct gtggaacatg atgattggcg cgccactggt
    40681 cgccattctg gcgtcagcgg tgggttatct ggcaacggcg caggcactgt tagcgaggct
    40741 tgaaacctcg gttgccatct ggttcctgct actggtggtt tatcacgtta tccgccgctg
    40801 gatgctgatc cagcgtcgca ggctggcgtt tgaccgggcg aagcatcgcc gggcagagat
    40861 gttagcgcaa cgcgcgcgtg gcgaagagga agcgcatcat cacagtagcc cggaaggggc
    40921 aattgaagtc gatgaaagcg aagtcgatct cgatgccatc agtgcgcaat ccttgcggct
    40981 ggtgcgctca attttgatgt tgatcgccct gctttctgtc attgtgctgt ggtcagaaat
    41041 ccattccgct ttcggcttcc tcgaaaatat ttcgctgtgg gatgtcacct ccacggtaca
    41101 gggcgtagaa agtctggagc caattaccct cggtgcggtg ctgattgcca ttctggtgtt
    41161 tatcatcacc acgcagctgg tgcgcaacct gcccgcgctg ctggaactgg cgattttgca
    41221 gcacctggat ttaacgccgg gtacgggcta cgccatcacc accatcacca aatatctgct
    41281 gatgctgatt ggcgggctgg tcggcttctc gatgattggt attgagtggt cgaaattgca
    41341 gtggctggtt gccgcgctcg gtgttggtct cggttttggt ttgcaggaga ttttcgccaa
    41401 ctttatctct ggcctgatta tcctgttcga aaaaccgatt cgcattggcg atacggtaac
    41461 gatccgcgat cttaccggta gcgtgacgaa aattaacacc cgcgccacca ccatcagcga
    41521 ctgggaccgt aaagagataa tcgtgccgaa caaggcgttt attaccgagc agtttatcaa
    41581 ctggtcgctc tctgactcgg tcacgcgcgt ggtgttgacg ataccggccc ctgccgatgc
    41641 caatagcgaa gaagtgacgg aaatcctgct caccgcagcg cgtcgctgct cgctggtgat
    41701 cgacaacccg gcaccggaag tcttcctggt ggatctgcaa caggggattc agattttcga
    41761 gctgcgtatt tacgccgctg agatgggtca ccgtatgccg ctacgccatg agatccacca
    41821 gctgattctg gcaggcttcc acgcccacgg tatcgatatg ccattcccgc ccttccagat
    41881 gcgtctggaa agcctcaacg gtaagcaaac ggggagaaca ttaacttctg cgggcaaagg
    41941 tcgtcaggcg ggaagtttgt aatgcatgaa gctgccaaat gatgtcaacg tggctcattt
    42001 agcagcttaa catcatcttc agttatcacc ggccctggag agtatcatcc atacgcatta
    42061 cttcctgaca actcatcgcc ggagagacga tgagttgcag gatttcatat gatcagtcga
    42121 gagctaactc aggtttattc gccatgcgac tgcaatagtt ctggtaaatc gccatcgcta
    42181 acagcgagaa gaagacaggg ccgccaatca tccacaatgt gctgtcccag tctccggctt
    42241 caaccacagg ttgaatgatg gtgaagacgt tcgcaaatgt caccaccagt acgacaacca
    42301 ccgtcgcaat cattgccgac atacgcgttt tgaaaatcac aaatggtctg tcgagatcct
    42361 gacgtgcttt aaagaatggg aaagccagcg cgaggaacag gtaaggaagc gtcatagaca
    42421 cgttcgccat cagcgtcagc ttgttaaaga acgcggatgc ggtaccgcca ccaaacgaaa
    42481 ccagcaggat gaagacagta accaacccgc actgcatcca catggcgata gacggcatcc
    42541 ccatcgcatt caggcgcgtc atcggttccg gccacaacgc tttcggcgtc ccctggatga
    42601 tggctttcag cggtgaatag caaagcgtaa agaacgcacc ggtataagcg aggaacatcg
    42661 aaagtccggt aatacgcgca aaccatacgc ccagcgacaa tgacgcttca ggtgacaaat
    42721 gcagtgcgtt acccagcgtc accccgaggc tcttcatcag cacataggta atattgccaa
    42781 ggttaacgga accattactt aatacctgct gccagtttgt gctgacaccc cataaaaata
    42841 ttgccagcga ataaccgatt gaaataacaa tagcggcaaa aacaatacct ttggcaaagt
    42901 tcttttctgg attttccgtt ttatcgacca gaccaccaac cgcttcaatt ccgccatagg
    42961 caaaaatagc aaataccaca aacgatagca ttgccagacc ggactgataa cctgggttcg
    43021 gtgatgcaag gaaattaata tcctgcgcga aatgcccacc atttaataac aaaatagtaa
    43081 tgcttactaa cagcaatact aaattcagac acattactgc aataccgccc accgcagtaa
    43141 tgcgggcaat tttattaatc ccctttgaag caacgacggt gaccagaatc atccatgcca
    43201 ctgccagcag accaaccacc tgcgtaggct ccagtccagc aatacgccag tgctgggtca
    43261 tgtcgctacc atagaggaat gttgagaacg gtacccaaac tttcgcggag gtactcacca
    43321 tccagatgat ataagaggaa aaccacataa acgtaccaat gaaggcaaaa cgtggtccga
    43381 cactattatt catccaggaa tagataccac cttcttcttt gcgataagca gctcccattt
    43441 cagccatcat taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa
    43501 tcgcactata acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag
    43561 taaagatcat caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac
    43621 catccttcat ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga
    43681 gcaggcagcg taaaaaccaa tacagaattg atacctggat gatatcaaat atccagtctt
    43741 aaattgtggc ctttaacgga aaaatgaaaa catatgctac agaatggcgc ttatcacgcc
    43801 attcttcgct gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat
    43861 gacttcagcc agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc
    43921 cacgccggaa cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg
    43981 gtgctgcggc aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg
    44041 ctcacgggca tccgtcaatt catggaaacc attcgccagc tcaatacctt tataataaac
    44101 ctcaaagcgt tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct
    44161 ggctggaaag tggtacacaa aggtcggttt ttctttgcca atatttggct ctacgccaaa
    44221 ggtaaacagc aattgtagca gcgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa
    44281 atccagtttc gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc
    44341 cagataacgc aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac
    44401 ctgttgtaag agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata
    44461 ccactccagc atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct
    44521 gcggcacagc tggaataccg gcccacaacc ggcaaccagc aggcgtttca tatggtattc
    44581 cgggctggtc attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt
    44641 ctcaaacggg accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac
    44701 ctccagcact ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt
    44761 taataagtta ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc
    44821 tccagttttt gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg
    44881 caggaatcaa acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt
    44941 aaattaatca tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc
    45001 aaatttcaga cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc
    45061 gttcgcagac cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca
    45121 atctggagga atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc
    45181 gcgggattac gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta
    45241 atctcaaaag tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct
    45301 gtcgcgcagg atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac
    45361 tggttgtgtg agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc
    45421 caactggaac tgtgggggtg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt
    45481 cgcttcggcg gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat
    45541 atgctgcaca cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa
    45601 catttcgtgc tggatattct ggttgatgat ggtcatgttc gcggcgtggt agcaatgaac
    45661 atgatggaag gcacgctggt gcagatccgt gctaacgcgg tcgttatggc tactggcggt
    45721 gcgggtcgcg tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt
    45781 atggcgctaa gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc
    45841 ggtctgccag gttccggtat cctgatgacc gaaggttgcc gtggcgaagg cggtattctg
    45901 gtcaacaaaa atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg
    45961 ggcgagccga aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc
    46021 tggcacgaat ggcgtaaagg caacaccatc tccacgccgc gtggcgatgt ggtttatctc
    46081 gacctgcgtc acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg
    46141 gcgaaagcgt acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgaccgca
    46201 cactacacca tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg
    46261 ttcgccgtgg gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggttctaac
    46321 tccctggcgg aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca
    46381 gcaactgccg gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa
    46441 cgtctgaaag atctggttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa
    46501 atgggcctgg caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa
    46561 accatcgaca agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc
    46621 tccagcgtgt tcaacaccga cctgctctac accattgaac tgggccacgg tctgaacgtt
    46681 gctgaatgta tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt
    46741 ctggacgaag gttgcaccga gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc
    46801 cgcgatgctg atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg
    46861 ccagctaaac gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat
    46921 aagaaggaga aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa
    46981 cccggaagtc gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac
    47041 ctcattactg gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg
    47101 ctggtcctgc cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa
    47161 actggcatgt aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc
    47221 taacttcccg attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga
    47281 agcgatcaaa ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca
    47341 gaccccggcg cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg
    47401 ctacgccgcg tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac
    47461 gctggcgcat cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca
    47521 gttgaacagc cagaacggcg tatggagctg tactttcgtg ggctattgct ccgaagtctg
    47581 cccgaaacac gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga
    47641 ctttcttatc gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac
    47701 cgtatgtacg gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca
    47761 tgctgcgcga aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc
    47821 tgtttgccct gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc
    47881 cggttatcgt gatcattaac ctgatcactc tggcggcagc tctgctgcat accaaaacct
    47941 ggtttgaact ggcaccgaaa gcagccaata tcattgtaaa agacgaaaaa atgggaccag
    48001 agccaattat caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg
    48061 ttgccctgta ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa
    48121 ccggtattct ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg
    48181 atgatcctgc tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc
    48241 tacgagcgcg ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg
    48301 atcgttctgc cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa
    48361 attcacgtgc ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc
    48421 acgctgattg gtatcgttac aatctaacgt atcgccaatg taaatccggc ccgcctatgg
    48481 cgggccgttt tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa
    48541 ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca
    48601 caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc
    48661 agggtaaacc ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca
    48721 cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg
    48781 gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cacaacaaaa tactggcctg
    48841 aacttaccgc taaacagtgg aatgggatca cactattaca tctcgcaacc tacactactg
    48901 gcggcctgcc attgcaggtg ccggatgagg tgaaatcctc aagcgacttg ctgcgcttct
    48961 atcaaaactg gcagcctgca tgggcgccag gaacacaacg tctgtatgcc aactccagta
    49021 tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc
    49081 aaacacgtgt cttccagcca ctcaaactca accatacgtg gattaatgta ccgcccgcag
    49141 aagaaaagaa ttacgcctgg ggatatcgcg aaggcaaggc agttcatgtt tcgccagggg
    49201 cgttagatgc tgaaacttat ggtgtgaagt cgaccattga agatatggcc tgctgggtac
    49261 gaagcaatat gaatcaccgt gatatcaacg acaaaacact tcaacaaggg atacaactgg
    49321 cacaatctcg ctactggcaa accggcgata tgtatcaggg tctgggctgg gaaatgctgg
    49381 actggccggt aaatcctgac atcatcgtta acggcagtga caataaaatt gcactggcag
    49441 cacgccccgt aaaagcgatt acgcccccaa ctcctgcagt acgcgcatca tgggtgcata
    49501 aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg
    49561 gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctggc
    49621 agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg
    49681 cttttccttg ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg
    49741 ctccctctcg ttgccgcagc gacagctgca tttctggtcg ttgcctgcag ttctcctacg
    49801 ccgccgcgtg gcgtaacagt agtgaataat ttcgacgcca aacgttatct cggtacctgg
    49861 tatgagattg cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca
    49921 tacagcctgc gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga
    49981 ggaatgtggc agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg
    50041 ctgaaagtgt cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg
    50101 gaataccgcc atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc
    50161 acgccaacca tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg
    50221 tttgatgtca gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc
    50281 agaccaataa tccccaatac gattaacgcc agactcgcca ggcgcatcgg gttagcggac
    50341 tcaccgagca gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg
    50401 gcataagccg tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact
    50461 atcatcgccg tcacagtaat aacacttggc gtcaaacgac taaagccgtg ggtatatttc
    50521 aggccaacgg cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata
    50581 tcaggctccg gagaattggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc
    50641 aaagctgatg gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt
    50701 gtgactatcg caaaaaaaca aacggcacga cataactgtc gtaccgttat tgcttattgc
    50761 tgcgctttcg ttgctgcacc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg
    50821 ccacgcgtgg tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct
    50881 gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat
    50941 agcaaattaa taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag
    51001 agaagaaaaa ttagctggca gcgcgagaga tggagttgcc gagatgtttg atgtcttcgc
    51061 cgaaaccgcg agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga
    51121 taaggcgttt catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac
    51181 aaccgcatta cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt
    51241 cgccgatttg tacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt
    51301 tgccgccagt acctgcggta tcacctttca ggcccggatc ggtatcaacg atttccagtt
    51361 caacgaagtt cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact
    51421 ctgcctgatc cagcagccat ttagcgttgt cgccaattgc ttttgcatca gcagacagct
    51481 gctcgaaagt ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca
    51541 ggttcatatc gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta
    51601 cgcgagtacc ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg
    51661 gttttacgaa ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac
    51721 cagcacgaaa atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg
    51781 tagctaagcc acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg
    51841 ttaacgcaac ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct tttgaatata
    51901 gacgcggacg aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg
    51961 ccccgctcat ttatcgatcg catggagaaa ggcaatccgg acgatcctct tttgcgtcag
    52021 gtacttacct cgcaagatga gtttgtcgtc gcgtccggat tctccaccga cccgctggaa
    52081 gaacagcaca gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg
    52141 gtcaaaggcg gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc
    52201 gaaaatcagg gcaacaagcg taactggcag actgctcttg agtatgttgc tgcgcatccg
    52261 gaactggacg agatgatttt ctccggcggc gatccgctga tggcgaaaga tcacgagctg
    52321 gactggttgc tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc
    52381 cgtctgccga ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt
    52441 tctacgctgc aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg
    52501 ttccgtcagg cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt
    52561 ctgttacgtg gtgtgaacga taacgcacaa acgttggcaa acctgagtaa tgcgttgttc
    52621 gatgccggcg tgatgccgta ttacctgcat gtgctagata aagtacaggg cgcggcgcat
    52681 tttatggtga gtgatgacga agcacggcag attatgcgtg agttgctgac attggtgtcg
    52741 ggatatctgg tgccgaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg
    52801 gatctccagc tacgccagca gtaactgatt aaacagggaa gttaaacatt agcctgatgc
    52861 gctacgctta tcaggcctac attgtgcatt gaaattgaat ggatttgcaa tgaactgtag
    52921 gccggataag gcgtttacgc cgcatccggc aagagcaaca aacgcaagta cagcaacctg
    52981 tcattaaact gggaaattta atctttgccc gttgcataac ctttaattat tcatatacat
    53041 tgctgttgtt actctttata tagccaacac caataattaa cctttctttt ctgtttatat
    53101 agagtgagta cggctattta tgttatttta acaactatta ctaccaagcc catttatctg
    53161 gcttataaat taaaattaat tatttaattg agaaatggtt agggagaacc tacatggcca
    53221 taagcatcaa gggtgttaat accggtgtta ttcgcaagag taataatttc attgcactgg
    53281 cgctgaaaat taaagaacct cgcaataaag aatcgttgtt ctttatgtct gtcatggaac
    53341 tgcgcgacct gctgattgcg ctggaaagtc gtctgcatca aaagcataaa ctggatgctg
    53401 ctgcacgcct tcagtatgag caagctcgcg ataaagtcat taagaaaatg gcagaaaaca
    53461 tcccagaaat tctggttgat gagcttaaaa atgccgatat taatcgtcgg gttaacacgc
    53521 tggaacttac cgataatcaa ggcgaaaatc ttaccttcgt attaacgcta catgacggta
    53581 gcaaatgtga gttagtcgtc aatgagttgc agattgaaat gctggcgcga gcaatcattc
    53641 atgcaattaa taatgcggaa atgcgtgaac tggcattacg tattacctca ctgttagatt
    53701 tcctgccgtt atatgacgtt gactgccagg aaaatggcaa tctggaatat gacacctatt
    53761 cccaaccgga atggaaacat aacctgttcg accattatct ggcggtgctt taccgcttta
    53821 aagatgaaag tggcaaagaa caatttagcg gcgcagtggt gaaaacgcgt gaagcaacgc
    53881 cgggtaaaga aatagaggcg atcactcgcc gaatgcttga tttcagccca cgactgaaaa
    53941 aactcgcggg tgtgccatgt caggtctatg ttcgcaccgt agcggcgaat aacgcccaac
    54001 cgctaaccca ggatcaatgc ttacgcgcgc tgcatcactt acgtgttcaa tccaccagca
    54061 aaactgcacc acaggcgaaa taatctcatc tttgctaatg cgaggctgga gccatccagc
    54121 ctgaaatgac tgataacgtg atcgttggtc atgccggatg cggcgtaaac gccttatccg
    54181 gcctacaaat tctcaagcat tcaatcagtt gcaatgcctg ctgtaggcct gataagcgaa
    54241 gcgcatcagg caatgatacg tttgtcaccc agcctctgga tcacatcagt tcgggcactt
    54301 ataaacctga ccgataatct ggctatccgt cgggacaaaa ctggacaaca ttccctgcga
    54361 cgggctgctg atgccataaa tcacgttacc gcccattgca gccgcctggt tgcgcagatc
    54421 gtttgctgcg ccgcgcatag aaccgccctc ttctccgtgt tgcccggaaa gccagttgct
    54481 ttgcttacct gtcgcagtac caatcagctg gcactctgcg cctggctgct cgtccacaat
    54541 gcgtacactc tgaccggcag cactcaattc gttgctggag ctacaacccg ccatcagtag
    54601 cgcggcaccg acaatccctg ctaagtattt tacgtgcatg ttattcccca taatcaatga
    54661 gctggacgcc actcgcgtcg tccgtgtctg aatcttatac tagaaagatg accaaaagaa
    54721 aaacccccag acatttctgc ccgggggttt gtttatttct gcgaggtgca gggcaattac
    54781 atcatgccgc ccatgccacc catgccgccc ataccgccag cagcgcctaa gtcagctgca
    54841 tcatttttcg gcaggtcggt aaccatgcac tcggtggtga tcatcaggcc agccacagaa
    54901 gccgcgtact gcagagcaga acgggttact ttggttgggt ccaggatacc catgtcgatc
    54961 atgttgccgt attcttcggt tgctgcgttg taaccgtagt tgccgtcgcc gcctttaacg
    55021 gtgttagcaa caacagacgg ttcttcgccg cagttcagga cgatctgacg cagcggagct
    55081 tccattgcac gcagtgcaac tttgataccc acgttctggt cttcgttctg accacgcagg
    55141 tcagccagtt tagacgctac gcggatcagc gcaacaccac caccagcaac cacgccttct
    55201 tctaccgcag cacgggtcgc gtgcagggcg tcttcaacgc gtgctttttt ctctttcatt
    55261 tcaacttcgg tagcagcacc tactttgata actgcaacgc cgcctgccag tttcgctacg
    55321 cgttcctgca gtttttcacg gtcgtagtca gaagttgctt cttcaatctg ctgacggatc
    55381 tgagcaacac ggccctggat tgcagcttct tcacccacgc catcaatgat ggtggtggtg
    55441 tctttgttga tcacaacacg tttagcctga cccaggtctt ccagggttgc tttttccagc
    55501 tccataccga tctcttcaga gattacggta ccgccagtca gggttgcgat atcctgcagc
    55561 atagctttac gacgatcgcc gaagcccgga gctttaaccg cagcaacttt cacgatgcca
    55621 cgcatggtgt taacaaccag agttgccaga gcttcgcctt caacatcttc agcgatgatc
    55681 agcagcggtt tgcctgcttt ggcaacagct tccagaaccg gcagcatttc gcggatgtta
    55741 gagattttct tgtcagccag caggatgaac gggctttcca gttctactgc gccagtttcc
    55801 ggcttgttga tgaagtaagg agacaggtag ccacggtcga actgcatacc ttcaaccacg
    55861 tccagttcgt cctgcagacc ggtaccgtct tcaacggtga taacgccttc tttaccgact
    55921 ttgtccatcg cttcagcgat cagtttacct acggtttcgt cggagttagc ggagatggta
    55981 ccaacctgag caatcgcttt agagtcagag cacggtacgg acagcgcttt cagttcttca
    56041 actgcagcgg taacggcttt gtcgatacca cgtttcaggt ccatcgggtt catgcccgca
    56101 gcaacagctt tcagaccctc agtgatgata gcctgagcca gtacagttgc agtggtggta
    56161 ccgtcgcctg cagcgtcgtt cgctttagag gcaacttctt tcaccatctg cgcacccata
    56221 ttttcgaact tgtcttccag ttcgatttca cgagcaacgg aaacaccatc tttggtgatg
    56281 gtcggtgcac cgaaagattt atccagaact acgttacggc ctttcggacc gagggtaact
    56341 ttcactgcat ctgccagtac gtttacgccg cgcagcattt tcacacgagc gtcgttaccg
    56401 aattttacgt ctttagctgc cattatcttt attccttaaa ttcgtatgtt cagtgtcgtg
    56461 cgaggattac gcttcaacaa ttgccagaat gtcgctttcg gacatgatca acacttcttc
    56521 attgtcgatc ttctcagatt tcacaccgta gccatcgttg aaaataacga tgtcgccaac
    56581 tttcacatcc agcggcttca cttcgccatt ttcaaggata cggccattgc cgacagccag
    56641 cacttcgccg cgggtggatt tagccgctgc agagccggtc agaacgatgc cgccagcaga
    56701 tttagtttca acttctttac gcttgacgat cacgcgatca tgcaatggac gaatattcat
    56761 tgataactct cctttgagaa agtccgtatc tgttatgggt gacgccggag cttacgtggt
    56821 ttcccggctg gtgaccagag aaatggggat gaggcttcgc cccttcaagg gggaaaaaga
    56881 aaaaaaattc tgatcagcac aaaatcgggt gaaaaccctg attcacctca catttcatcg
    56941 caatttcttc atcgccgtca taagcgaatc tgattgtgct accatcgaaa atctacgcag
    57001 ttgcccaaaa tttgggcgca atcggacatc aacccatgag tggactcaaa caagaactgg
    57061 ggctggccca gggcattggc ctgctatcga cgtcattatt aggcactggc gtgtttgccg
    57121 ttcctgcgtt agctgcactg gtagcaggca ataacagcct gtgggcgtgg cccgttttga
    57181 ttatcttagt gttcccgatt gcgattgtgt ttgcgattct gggtcgccac tatcccagcg
    57241 caggcggcgt cgcgcacttc gtcggtatgg cgtttggttc gcggcttgag cgagtcaccg
    57301 gctggctgtt tttatcggtc attcccgtgg gtttgcctgc cgcactacaa attgccgccg
    57361 ggttcggcca ggcgatgttt ggctggcata gctggcaact gttgttggca gaactcggta
    57421 cgctggcgct ggtgtggtat atcggtactc gcggtgccag ttccagtgct aatctacaaa
    57481 ccgttattgc cggacttatc gtcgcgctga ttgtcgctat ctggtgggtg ggcgatatca
    57541 aacctgcgaa tatccccttc ccggcacctg gtaatatcga acttaccggg ttatttgctg
    57601 cgttatcagt gatgttctgg tgttttgtcg gtctggaagc atttgcccat cttgcctcgg
    57661 aatttaaaaa tccagagcgt gattttcctc gcgcattgat gattggtctg ctgctggcag
    57721 gattagtcta ctggggctgt acggtagtcg tcttacactt cgacgcctat ggtgaaaaaa
    57781 tggcggcggc agcatcgctt ccaaaaattg tagtgcagtt gttcggtgta ggagcgttat
    57841 ggattgcctg cgtgattggc tatctggcct gctttgccag tctcaacatt tatatacaga
    57901 gcttcgcccg cctggtctgg tcgcaggcgc aacataatcc tgaccactac ctggcacgcc
    57961 tctcttctcg ccatatcccg aataatgccc tcaatgcggt gctcggctgc tgtgtggtga
    58021 gcacgttggt gattcatgct ttagagatca atctggacgc tcttattatt tatgccaatg
    58081 gcatctttat tatgatttat ctgttatgca tgctggcagg ctgtaaatta ttgcaaggac
    58141 gttatcgact actggcggtg gttggcgggc tgttatgcgt tctgttactg gcaatggtcg
    58201 gctggaaaag tctctatgcg ctgatcatgc tggcggggtt atggctgttg ctgccaaaac
    58261 gaaaaacgcc ggaaaatggc ataaccacat aatccggcgt ttcgacatta atcctggcga
    58321 tcgtctttat gatcaaggcg gtcgcgctca tcatcctttc gctggtactc accatcaaaa
    58381 gtattaccgc caccggtccc ggcgctaaaa ccgccgcctg gcatacgaga aaagcgcaaa
    58441 tgcggcatca acttcactgt cagatgcttt tgcaccggcg gtaataaaag cagaagaccg
    58501 aggaagtcgg tgaaaaagcc cggtaataaa agcagcaaac cggcaatgat cagcgaaaca
    58561 cttttaatca tctccgccgc tgggttttca cccgccgcca ttttttgctg catcagcacg
    58621 aaattcttaa agccctggtt acgtaccagt gacatcccga taactgacgt gaatataacc
    58681 agcacgaggg tcagcaatac ccccaataca tgggcaacct gaataaagat tgaaatctca
    58741 atatagacat aaaggaaaat ggcaataaaa ggtaaccagc gcaaaggttt ctcctgtaat
    58801 agcagccggt aaaacccggc tacctgaatg ggttgcgaat cgcgttcagc ttatattgtg
    58861 gtcattagca aaatttcaag atgtttgcgc aactattttt ggtagtaatc ccaaagcggt
    58921 gatctatttc acaaattaat aattaagggg taaaaaccga cacttaaagt gatccagatt
    58981 acggtagaaa tcctcaagca gcatatgatc tcgggtattc ggtcgatgca ggggataatc
    59041 gtcggtcgaa aaacattcga aaccacatat attctgtgtg tttaaagcaa atcattggca
    59101 gcttgaaaaa gaaggttcac atgtcaaaca acattcgtat cgaagaagat ctgttgggta
    59161 ccagggaagt tccagctgat gcctactatg gtgttcacac tctgagagcg attgaaaact
    59221 tctatatcag caacaacaaa atcagtgata ttcctgaatt tgttcgcggt atggtaatgg
    59281 ttaaaaaagc cgcagctatg gcaaacaaag agctgcaaac cattcctaaa agtgtagcga
    59341 atgccatcat tgccgcatgt gatgaagtcc tgaacaacgg aaaatgcatg gatcagttcc
    59401 cggtagacgt ctaccagggc ggcgcaggta cttccgtaaa catgaacacc aacgaagtgc
    59461 tggccaatat cggtctggaa ctgatgggtc accagaaagg tgaatatcag tacctgaacc
    59521 cgaacgacca tgttaacaaa tgtcagtcca ctaacgacgc ctacccgacc ggtttccgta
    59581 tcgcagttta ctcttccctg attaagctgg tagatgcgat taaccaactg cgtgaaggct
    59641 ttgaacgtaa agctgtcgaa ttccaggaca tcctgaaaat gggtcgtacc cagctgcagg
    59701 acgcagtacc gatgaccctc ggtcaggaat tccgcgcttt cagcatcctg ctgaaagaag
    59761 aagtgaaaaa tatccaacgt accgctgaac tgctgctgga agttaacctt ggcgcaacag
    59821 caatcggtac tggtctgaac acgccgaaag agtactctcc gctggcagtg aaaaaactgg
    59881 ctgaagtcac tggcttccca tgcgtaccgg ctgaagacct gatcgaagcg acctctgact
    59941 gcggcgctta tgttatggtt cacggcgcgc tgaaacgcct ggctgtgaag atgtccaaaa
    60001 tctgtaacga cctgcgcttg ctctcttctg gtccacgtgc cggcctgaac gagatcaacc
    60061 tgccggaact gcaggcgggc tcttccatca tgccagctaa agtaaacccg gttgttccgg
    60121 aagtggttaa ccaggtatgc ttcaaagtca tcggtaacga caccactgtt accatggcag
    60181 cagaagcagg tcagctgcag ttgaacgtta tggagccggt cattggccag gccatgttcg
    60241 aatccgttca cattctgacc aacgcttgct acaacctgct ggaaaaatgc attaacggca
    60301 tcactgctaa caaagaagtg tgcgaaggtt acgtttacaa ctctatcggt atcgttactt
    60361 acctgaaccc gttcatcggt caccacaacg gtgacatcgt gggtaaaatc tgtgccgaaa
    60421 ccggtaagag tgtacgtgaa gtcgttctgg aacgcggtct gttgactgaa gcggaacttg
    60481 acgatatttt ctccgtacag aatctgatgc acccggctta caaagcaaaa cgctatactg
    60541 atgaaagcga acagtaatcg tacagggtag tacaaataaa gaaggcacgt cagatgacgt
    60601 gccttttttc ttgtgagcag taacttaaaa ataacaacct aatatcaact tgttaaaaaa
    60661 caaggaaggc taatatgcta gttgtagaac tcatcatagt tttgctggcg atcttcttgg
    60721 gcgccagatt ggggggaata ggtattggtt ttgcaggcgg attgggggtg ctggttcttg
    60781 ccgctattgg cgttaaaccc ggtaacatcc cgttcgatgt catttccatt atcatggcgg
    60841 ttatcgccgc tatttctgcc atgcaggttg ctggcggtct ggactatctg gttcatcaga
    60901 cagaaaagct gctgcgccgt aacccgaaat acatcacgat cctcgcaccg atcgtgacct
    60961 atttcctgac tatctttgct ggtactggca acatatctct ggcgacactg ccagttatcg
    61021 ctgaagttgc gaaggaacaa ggcgtcaaac cttgccgtcc gctgtctact gcagtggtat
    61081 ccgcgcagat tgcgatcacc gcatcgccaa tctcagcggc agtggtttac atgtcttccg
    61141 tgatggaagg tcatggcatc agctacctcc atctgctctc cgtggtcatc ccgtccaccc
    61201 tgctggcggt tctggtgatg tcctttctgg tcactatgct gttcaactcc aaactctctg
    61261 acgatccgat ttatcgcaag cgtctggaag agggcctggt tgaactgcgc ggtgaaaagc
    61321 agattgaaat caaatccggt gcaaaaacgt ccgtctggct gttcctgctg ggcgtagttg
    61381 gcgtggttat ctatgcaatc atcaacagcc caagcatggg tctggttgaa aaaccgctga
    61441 tgaacaccac caacgcaatc ctgatcatca tgctcagcgt tgcaactctg accaccgtta
    61501 tctgtaaagt cgataccgac aacatcctca actccagcac cttcaaagca ggtatgagcg
    61561 cctgtatttg tatcctgggt gttgcgtggc tgggcgatac tttcgtttcc aacaacatcg
    61621 actggatcaa agataccgct ggtgaagtga ttcagggtca tccgtggctg ctggccgtca
    61681 tcttcttctt tgcttctgct ctgctgtact ctcaggctgc aaccgcaaaa gcactgatgc
    61741 cgatggctct ggcactgaac gtttctccgc tgaccgctgt tgcttctttc gctgcggtgt
    61801 ctggtctgtt cattctgcca acctacccga cgctggttgc tgcggtacag atggatgaca
    61861 cgggtactac ccgtatcggt aaattcgtct tcaaccatcc gttcttcatc ccgggtactc
    61921 tgggtgttgc cctggccgtt tgcttcggct tcttgctggg tagcttcatg ctgtaatgac
    61981 caatcgcggg gcgttcacgc cccgctttct ttcccgccga ctaacatcct tcccccgtcc
    62041 gttgtatagt gacctctctc ttgcggttcc atctgttctt gcgaggtgtt tatgcttgat
    62101 gaaaaaagtt cgaataccgc gtctgtcgtg gtgctatgta ccgcaccgga tgaagcgaca
    62161 gcccaggatt tagccgccaa agtgctggcg gaaaaactgg cggcctgcgc gaccttgatc
    62221 cccggcgcta cctctctcta ttactgggaa ggtaagctgg agcaagaata cgaagtgcag
    62281 atgattttaa aaactaccgt atctcaccag caggcactgc tggaatgcct gaagtctcat
    62341 catccatatc aaaccccgga acttctggtt ttacctgtta cacacggaga cacagattac
    62401 ctctcatggc tcaacgcatc tttacgctga tcctgctact ttgcagcact tccgtttttg
    62461 ccggattatt cgacgcgccg ggacgttcac aatttgtccc cgcggatcaa gcctttactt
    62521 ttgattttca gcaaaaccaa catgacctta atctgacctg gcagatcaaa gacggttact
    62581 acctctaccg taaacagatc cgcattacgc cggaacacgc gaaaattgcc gacgtgcagc
    62641 tgccgcaagg cgtctggcat gaagatgagt tttacggcaa aagcgagatt taccgcgatc
    62701 ggctgacgct tcccgtaacc atcaaccagg cgagtgcggg agcgacgtta actgtcacct
    62761 accagggctg tgctgatgcc ggtttctgtt atccgccaga aaccaaaacc gttccgttaa
    62821 gcgaagtggt cgccaacaac gcagcgtcac agcctgtgtc tgtttcgcag caagagcagc
    62881 acaccgcgca attgcccttt tccgcgctct gggcgttgtt gatcggtatt ggtatcgcct
    62941 ttacgccatg cgtgctgcca atgtacccac tgatttctgg catcgtgctg ggtggtaaac
    63001 agcggctctc cactgccaga gcattgttgc tgacctttat ttatgtgcag gggatggcac
    63061 tgacttacac ggcgctgggt ctggtagttg ccgccgcagg gttacagttc caggcggcgc
    63121 tacagcaccc atacgtgctc attggcctcg ccatcgtctt taccttgctg gcgatgtcaa
    63181 tgtttggctt gtttactcta caactcccct cttcgctgca aacacgtctc acgttgatga
    63241 gcaatcgcca acagggcggc tcacctggcg gtgtgtttgt tatgggggcg attgccggac
    63301 tgatctgttc accatgcacc accgcaccgc ttagcgcgat tctgctgtat atcgcccaaa
    63361 gcgggaacat gtggctgggc ggcggcacgc tttatctcta tgcgttgggc atgggcctgc
    63421 cgctgatgct aattaccgtc tttggtaacc gcttgctgcc gaaaagcggc ccgtggatgg
    63481 aacaagtcaa aaccgcgttt ggttttgtga tcctcgcact gccggtcttc ctgctggagc
    63541 gagtgattgg tgatgtatgg ggattacgct tgtggtcggc gctgggtgtc gcattctttg
    63601 gctgggcctt tatcaccagc ctacaggcta aacgcggctg gatgcgcgtg gtgcaaataa
    63661 tcctgctggc agcggcattg gttagcgtgc gcccacttca ggattgggca tttggtgcga
    63721 cgcataccgc gcaaactcag acgcatctca actttacaca aatcaaaacg gtagatgagt
    63781 taaatcaggc gctcgttgaa gccaaaggca aaccggtgat gttagatctt tatgccgact
    63841 ggtgcgtcgc ctgtaaagag tttgagaaat acaccttcag cgacccgcag gtgcaaaaag
    63901 cgttagcaga cacggtctta cttcaggcca acgtcacggc caacgacgca caagatgtgg
    63961 cgctgttaaa gcatcttaat gtccttggcc taccgacaat tctctttttt gacggacaag
    64021 gccaggagca tccacaagca cgcgtcacgg gctttatgga tgctgaaacc ttcagcgcac
    64081 atttgcgcga tcgccaaccg tgaacgacac ttgcagtggg aaagacggag gaaaatagcg
    64141 tgcaacgtga agatgtactg ggagaagccc tgaaattatt agaattacaa gggattgcca
    64201 acaccacgct ggagatggtt gctgaacgtg tggattatcc actggacgag ctacgccgct
    64261 tctggccaga caaagaggca atcctctacg atgcgctgcg ttatcttagc caacagatag
    64321 atgtctggcg tcgtcagctg atgctggacg aaacgcaaac cgccgaacaa aagctgctgg
    64381 cacgttatca ggcgttatcg gagtgcgtta aaaacaaccg ctatccgggc tgtctattta
    64441 tcgctgcctg tacgttttat cccgatcctg gccaccctat tcatcaactg gccgatcagc
    64501 aaaaaagcgc ggcctacgat ttcacccacg aactgttaac cacactggaa gttgacgatc
    64561 cggcgatggt agcaaagcag atggaactgg tgctggaagg ctgtttaagc cgaatgctgg
    64621 tgaatcgtag ccaggcggat gtcgacactg cacatcggct ggcggaagat atcctgcgct
    64681 tcgcccgctg ccgccagggt ggtgcattga cctgacagaa acacagaaaa gaagcgattt
    64741 gccgcaatct taagcagttg aatcgctttt actgaaatta ggttgacgag atgtgcagat
    64801 tacggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
    64861 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatataaac ggacctccac
    64921 ggaggtccgt ttttcgtttc aggacgccac gatttaagcg ttctgcctcc aaatcaattc
    64981 tcccgaactc aaccagattc tcccctcatc accagcaatt tgcgggcata tcccaattcg
    65041 ggaaaatttg ttcctgagcg atagcactaa ctgacgtgaa atgtcgtgcg gccccgtgat
    65101 gttgttgaac gtcaaatgac gtcatcagga gcgtaacgca tccataaagc acaacaccgg
    65161 tctgaacgcc gactgatgag attttctgaa tgagaacaaa gagaaatgta tcagtccgct
    65221 tgctcatgca aagactaaca atccattaaa atagtaagcg ctccggacaa ttttccatgg
    65281 attattttct gaacattttt ctttggcaaa gatgatgaat tttgatggta aggaatatta
    65341 cttctggttc tcagtaaaat cctttcgtaa tactatgtaa tcaagaagtt tatggctggt
    65401 aaaaataacg tcttacattc accaataata tgtaaataaa cccatctata gatggtaaaa
    65461 ataggttgtg gcaattatca ttgcatcatt cccttttcga atgagtttct attatgcaac
    65521 aacctgtagt tcgcgttggc gaatggcttg ttactccgtc cataaaccaa attagccgca
    65581 atgggcgtca acttaccctt gagccgagat taatcgatct tctggttttc tttgctcaac
    65641 acagtggcga agtacttagc agggatgaac ttatcgataa tgtctggaag agaagtattg
    65701 tcaccaatca tgttgtgacg cagagtatct cagaactacg taagtcatta aaagataatg
    65761 atgaagatag tcctgtctat atcgctactg taccaaagcg cggctataaa ttaatggtgc
    65821 cggttatctg gtacagcgaa gaagagggag aggaaataat gctatcttcg cctcccccta
    65881 taccagaggc ggttcctgcc acagattctc cctcccacag tcttaacatt caaaacaccg
    65941 caacgccacc tgaacaatcc ccaattaaaa gcaaacgatt cactaccttt tgggtatggt
    66001 tttttttcct gttgtcgtta ggtatctgtg tagcactggt agcgttttca actcttgata
    66061 cacgtcttcc tatgagcaaa tcgcgtattt tgctcaatcc acgcgatatt gacattaata
    66121 tggtaaataa aagttgtaac agctggagtt ccccgtatca gctctcttac gcgataggcg
    66181 tgggtgattt ggtggcgaca tcacttaaca ccttctccac ctttatggtg catgacaaaa
    66241 tcaactacaa cattgatgaa ccgagcagtt ccggtaaaac attatctatt gcctttgtta
    66301 atcagcgcca ataccgtgct caacaatgct ttatgtcgat aaaattggta gacaatgcag
    66361 atggttcaac catgctggat aaacgttatg tcatcactaa cggtaatcag ctggcgattc
    66421 aaaatgattt actggagagt ttatcaaaag cattaaacca accgtggcca caacgaatgc
    66481 aggagacgct ccagcaaatt ttgcctcatc gtggtgcgtt attaactaat ttttatcagg
    66541 ctcatgatta tttactgcat ggcgatgaca aatcattgaa tcgtgccagt gaattattag
    66601 gtgagattgt tcaatcatcc ccagaattta cctacgcgag agcagaaaaa gcattagttg
    66661 atatcgtgcg ccattctcaa catcctttag atgaaaaaca attagcagca ctgaacacag
    66721 aaatagataa cattgttaca ctgccggaat tgaataacct gtccattata tatcaaataa
    66781 aagcggtcag tgctctggta aaaggtaaaa cagatgagtc ttaccaggcg ataaatactg
    66841 gcattgatct tgaaatgtcc tggctaaatt atgtgttgct tggcaaagtt tatgaaatga
    66901 aggggatgaa ccgggaagcg gctgatgcat atctcaccgc ctttaattta cgcccagggg
    66961 caaacaccct ttactggatt gaaaatggta tattccagac ttctgttcct tatgttgtac
    67021 cttatctcga caaatttctc gcttcagaat aagtaacccc cgggttgatt catgctcggg
    67081 aatatttgtt gttgagtttt tgtatgttcc tgttggtata atatgttgcg gcaatttatt
    67141 tgccgcataa tttttattac ataaatttaa ccagaagacg tcacgcaatc tattgtaaac
    67201 attgaatgtt tatcttttca tgatatcaac ttgcgatcct gatgtgttaa taaaaaacct
    67261 caagttctca cttacagaaa cttttgtgtt atttcaccta atctttagga ttaatccttt
    67321 tttcgtgagt attcttagcg ccagtttggt ctggtcagga aatagttata catcatgacc
    67381 cggactccaa attcaaaaat gaaattagga gaagagcatg agttctgcca agaagatcgg
    67441 gctatttgcc tgtaccggtg ttgttgccgg taatatgatg gggagcggta ttgcattatt
    67501 acctgcgaac ctagcaagta tcggtggtat tgctatctgg ggttggatta tctctattat
    67561 tggtgcaatg tcgctggcgt atgtatatgc ccgactggca acaaaaaacc cgcaacaagg
    67621 tggcccaatt gcttatgccg gagaaatttc ccctgcattt ggttttcaga caggtgttct
    67681 ttattaccat gctaactgga ttggtaacct ggcgattggt attaccgcgg tatcttatct
    67741 ttccaccttc ttcccagtat taaatgatcc tgttccggcg ggtatcgcct gtattgctat
    67801 cgtctgggta tttacctttg taaatatgct cggcggtact tgggtaagcc gtttaaccac
    67861 tattggtctg gtgctggttc ttattccggt agtaatgacc gctattgttg gctggcattg
    67921 gtttgatgcg gcaacttatg cagctaactg gaatactgcg gataccactg atggtcatgc
    67981 gatcattaaa agtattctgc tctgcctgtg ggccttcgtg ggtgttgaat ccgcagcagt
    68041 aagtactggt atggttaaaa acccgaaacg taccgttccg ctggcaacca tgctgggtac
    68101 tggtttagca ggtattgttt acatcgctgc gactcaggtg ctttccggta tgtatccatc
    68161 ttctgtaatg gcagcttccg gtgctccgtt tgcaatcagt gcttcaacca tcctcggtaa
    68221 ctgggctgcg ccgctggttt ctgcattcac cgcctttgcg tgcctgactt ctctgggctc
    68281 ctggatgatg ttggtaggcc aggcaggtgt acgtgccgct aacgacggta acttcccgaa
    68341 agtttatggt gaagtcgaca gcaacggtat tccgaaaaaa ggtctgctgc tggctgcagt
    68401 gaaaatgact gccctgatga tcctcatcac tctgatgaac tctgccggtg gtaaagcatc
    68461 tgacctgttc ggtgaactga ccggtatcgc agtactgctg actatgctgc cgtacttcta
    68521 ctcttgcgtt gacctgattc gttttgaagg cgttaacatc cgcaactttg tcagcctgat
    68581 ctgctctgta ctgggttgcg tgttctgctt catcgcgctg atgggcgcaa gctccttcga
    68641 gctggcaggt accttcatcg tcagcctgat tatcctgatg ttctacgctc gcaaaatgca
    68701 cgagcgccag agccactcaa tggataacca caccgcgtct aacgcacatt aattaaaagt
    68761 attttccgag gctcctcctt tcattttgtc ccatgtgttg ggaggggcct tttttacctg
    68821 gagatatgac tatgaacgtt attgcaatat tgaatcacat gggggtttat tttaaagaag
    68881 aacccatccg tgaacttcat cgcgcgcttg aacgtctgaa cttccagatt gtttacccga
    68941 acgaccgtga cgacttatta aaactgatcg aaaacaatgc gcgtctgtgc ggcgttattt
    69001 ttgactggga taaatataat ctcgagctgt gcgaagaaat tagcaaaatg aacgagaacc
    69061 tgccgttgta cgcgttcgct aatacgtatt ccactctcga tgtaagcctg aatgacctgc
    69121 gtttacagat tagcttcttt gaatatgcgc tgggtgctgc tgaagatatt gctaataaga
    69181 tcaagcagac cactgacgaa tatatcaaca ctattctgcc tccgctgact aaagcactgt
    69241 ttaaatatgt tcgtgaaggt aaatatactt tctgtactcc tggtcacatg ggcggtactg
    69301 cattccagaa aagcccggta ggtagcctgt tctatgattt ctttggtccg aacaccatga
    69361 aatctgatat ttccatttca gtatctgaac tgggttctct gctggatcac agtggtccac
    69421 acaaagaagc agaacagtat atcgctcgcg tctttaacgc agaccgcagc tacatggtga
    69481 ccaacggtac ttccactgcg aacaaaattg ttggtatgta ctctgctcca gcaggcagca
    69541 ccattctgat tgaccgtaac tgccacaaat cgctgaccca cctgatgatg atgagcgatg
    69601 ttacgccaat ctatttccgc ccgacccgta acgcttacgg tattcttggt ggtatcccac
    69661 agagtgaatt ccagcacgct accattgcta agcgcgtgaa agaaacacca aacgcaacct
    69721 ggccggtaca tgctgtaatt accaactcta cctatgatgg tctgctgtac aacaccgact
    69781 ttatcaagaa aacactggat gtgaaatcca tccactttga ctccgcgtgg gtgccttaca
    69841 ccaacttctc accgatttac gaaggtaaat gcggtatgag cggtggccgt gtagaaggga
    69901 aagtgattta cgaaacccag tccactcaca aactgctggc ggcgttctct caggcttcca
    69961 tgatccacgt taaaggtgaa gtaaacgaag aaacctttaa cgaagcctac atgatgcaca
    70021 ccaccacttc tccgcactac ggtatcgtgg cgtccactga aaccgcagcg gcgatgatga
    70081 aaggcaatgc tggtaagcgt ctgatcaacg gttccattga acgtgcgatc aaattccgta
    70141 aagagatcaa acgtctgaga acggaatctg atggctggtt ctttgatgta tggcagccgg
    70201 atcatatcga taccactgaa tgctggccgc tgcgttctga cagcacctgg cacggcttca
    70261 aaaacatcga taacgagcac atgtatcttg acccgatcaa agtcaccctg ctgactccgg
    70321 ggatggaaaa agacggcacc atgagcgact ttggtattcc ggccagcatc gtggcgaaat
    70381 acctcgacga acatggcatc gttgttgaga aaaccggtcc gtataacctg ctgttcctgt
    70441 tcagcatcgg tatcgataag accaaagcac tgagcctgct gcgtgctctg actgacttca
    70501 aacgtgcgtt cgacctgaac ctgcgtgtga aaaacatgct gccgtctctg tatcgtgaag
    70561 atcctgaatt ctatgaaaac atgcgtattc aggaactggc tcagaatatc cacaaactga
    70621 ttgttcacca caatctgccg gatctgatgt atcgcgcatt tgaagtgctg ccgacgatgg
    70681 taatgactcc gtatgctgcg ttccagaaag agctgcacgg tatgaccgaa gaagtttacc
    70741 tcgacgaaat ggtaggtcgt attaacgcca atatgatcct tccatatccg ccgggagttc
    70801 ctctggtaat gccgggtgaa atgatcaccg aagaaagccg tccggttctg gagttcctgc
    70861 agatgctgtg tgaaatcggc gctcactatc cgggctttga aaccgatatt cacggtgcat
    70921 accgtcaggc tgatggccgc tataccgtta aggtattgaa agaagaaagc aaaaaataat
    70981 tagctcgtac aagggaagtg gcttgccact tccctttttt gctcataagg agaacacatg
    71041 aaaacaccct cacagccgcg cgcgatatac tatatcgtgg cgatccaaat ctgggagtac
    71101 ttcagttttt acggcatgcg tgccttactc attctctatc tcacccatca gcttggtttt
    71161 gatgataacc atgccatcag cctgttcagc gcatatgctt ctctggttta cgttacccct
    71221 attctcggcg gctggcttgc cgaccgcctg ctcggcaacc gcactgcagt gattgccggc
    71281 gcgctgttaa tgacccttgg ccatgtggtg ctgggcattg atacaaattc aacctttagc
    71341 ctgtatctgg cgctggcaat cattatttgt ggctacggtt tattcaaatc aaacatcagc
    71401 tgtttgcttg gcgagctcta cgacgagaac gatcatcgac gtgatggcgg tttttcgctg
    71461 ctgtatgctg cgggcaatat cggttctatc gccgccccaa tcgcctgcgg cctggctgct
    71521 cagtggtatg gatggcatgt tggctttgcc cttgcgggtg gcggcatgtt tatcggtttg
    71581 ttgattttct taagcgggca tcgtcatttc cagtccacgc gtagtatgga taaaaaagcg
    71641 ctcactagcg tgaaatttgc cttaccagta tggagctggt tagtggtgat gctctgttta
    71701 gccccagtat tttttaccct gctgctggag aacgactggt ccggatattt gctggcgatc
    71761 gtttgcctca ttgccgcaca aatcattgcc cgcatgatga tcaaattccc cgaacaccgc
    71821 cgtgctcttt ggcaaattgt attgttgatg tttgtcggga cgttgttctg ggtactggca
    71881 caacagggcg gcagtaccat cagcttgttt atcgatcgct ttgtgaatcg tcaggcattc
    71941 aatattgaag tacctacagc actattccag tcggtgaatg caattgcagt gatgctcgct
    72001 ggggttgtac tggcctggct ggcatcgcca gaaagccgcg gcaactcaac attgcgcgtc
    72061 tggctgaagt ttgcctttgg cttactgctg atggcttgtg gctttatgtt gttggcattt
    72121 gatgcccgac atgcagcggc tggcggtcaa gcgtcaatgg ccgtgatgat atccgggctg
    72181 gcgctaatgg gctttgccga actctttatt gacccggtgg cgattgcgca aatcacgcgt
    72241 ctgaaaatgt ctggcgtatt aaccggtatt tatatgctgg caacaggcgc ggtcgccaac
    72301 tggctggcag gcgtcgtggc acagcagacg acagagtcgc aaattagcgg tatggcaatt
    72361 gcagcttacc agcgattctt ttctcagatg ggagagtgga cgttggcttg tgtcgcgatc
    72421 atcgtcgtat tggcctttgc tacccgtttt ctgtttagca cgccgacgaa tatgatacag
    72481 gagagcaacg attaactgtc gtaaaaacaa ttggttatga ataaattaac tttcagttta
    72541 taacactagc aaatctagat taataagtca tttaaaccat tttgatggtt atttattagt
    72601 gatatcaact tgaggtaagc gttagtttcg ataagataaa ctgagttatt aatagtcgag
    72661 gcagataata cagtgtaccg atctgtctct ttatctacgc taaattgaaa gctggattta
    72721 gaggaaccaa aatgtctgaa caagaaacac ggggagccaa tgaggctatt gattttaacg
    72781 atgaactgag aaatcgccgc gaaaaactgg cggcactacg tcagcaaggt gtggcgtttc
    72841 ccaatgattt tcgccgcgac catacctctg accagctgca cgaagagttt gatgcgaagg
    72901 ataaccagga actggaatcc ttaaacattg aagtctcggt tgctggccga atgatgaccc
    72961 gtcgtatcat ggggaaagcc tcctttgtaa cgttgcagga tgtcggtggc cgtattcaac
    73021 tgtacgttgc aagagatagc ctgccagaag gtgtttataa cgatcagttt aaaaaatggg
    73081 atctgggtga cattatcggt gcccgcggta cgctgtttaa gacgcaaacc ggtgagcttt
    73141 ccattcactg tactgagttg cgcctgctga caaaagcact acgtccttta ccagataaat
    73201 tccatggtct gcaggatcag gaagtccgtt atcgtcaacg ttatctggac ctcatcgcta
    73261 acgataaatc ccgtcaaacg tttgttgtcc gttcaaaaat tctggccgct atccgccagt
    73321 tcatggtcgc gcgcggcttt atggaagtag aaaccccgat gatgcaggta attccaggtg
    73381 gggcatctgc tcgcccgttt attacccatc ataacgctct ggatttagat atgtacctgc
    73441 gtatcgcgcc ggagctgtat ctgaaacgtc tggttgtagg cggttttgaa cgggtattcg
    73501 aaatcaaccg taacttccgt aatgaaggta tttctgttcg ccataatcct gagttcacaa
    73561 tgatggaact ctacatggcg tatgcggatt accacgattt gattgaactg acagagtccc
    73621 tgttccgcac cctggcacaa gaggttctgg gtaccactaa agtcacttat ggcgagcatg
    73681 tgtttgattt cggcaaaccg tttgaaaaac tcaccatgcg cgaagcaatc aaaaaatatc
    73741 gtccagaaac cgatatggcc gacctggata attttgatgc tgctaaagca ttagctgaat
    73801 ctatcggaat tacggtagag aaaagctggg ggctgggacg tattgtcacg gagatctttg
    73861 atgaagtggc agaagcacat ctgattcagc caacctttat tacggaatat ccggcagaag
    73921 tgtcaccgct ggcgcgccgt aatgatgtta acccggaaat caccgaccgt tttgaattct
    73981 tcatcggtgg tcgtgaaatc ggtaatggtt ttagcgaatt aaacgacgca gaagatcagg
    74041 ctgaacgttt ccaggaacag gttaacgcta aagctgcagg tgacgacgaa gccatgttct
    74101 atgacgaaga ttacgtgact gcgctggaat atggtctgcc gccaaccgct ggtctgggta
    74161 ttggtatcga ccgaatgatt atgctgttta caaacagtca tactattcgc gacgttattc
    74221 tcttcccggc gatgcgccca cagaaataaa tttcacttta atgaacgaag cagtcaggcg
    74281 actgcttcgt tcgtcgttcc gcaaatccag ttttgaggag agacgcttcg tttcacgata
    74341 accagcgggt ccactcaact acagctccta aaagagagaa aaaagtaatg ccacaggcag
    74401 actcattggc caggttattc cgaccaggaa tgcactaagt aaacgaatat gtgttttctc
    74461 atgtgagata aaccagataa tgacaaagga tatgttcaca ccaatcacat aaaaaattaa
    74521 tattttagag aatagtgcca ttttttatgt tcttcttatc cttcctggct acttgtaaaa
    74581 ctgacggaaa tagtcatcaa ctgtcataat gttgaggtca aaatcaatat ccggtaagtc
    74641 taagcattta agcctgatta aatcttcgat ttcacttgcg gctaacgatg tcgttattgc
    74701 gaaggagttt tcattcaatt gtcgtggtat cccatattca tcagccacga tagaggagaa
    74761 attcatatca tacatcaatg atcttaacct taagaacact gaggaataag atgatggata
    74821 atcaaaagaa acaacataag tattgaactt gtttttacct tccataatat gactcctatc
    74881 aatattgaat gctctgggga cgcaaaaatt attaaccccg attgatgatt ctcatatagg
    74941 agatattctt ttttttgctc agtaaagggg atgacttggg acaaaaaaac gcgctctctg
    75001 cccattcttt gtgcatcccc ctacccttca gatgctctcc tgcatcactc cgcggagagc
    75061 atgaaaaata tcgtgactct gtactctccc atcaactgcg aatatcatca tactcccgcg
    75121 ttttatcaaa ttcgtgtttc gcaaatgggc atagtgggat aatttttcgt ttttcccgac
    75181 gcattttttc cacgactttc gcaaccagct gtttaccaat cccctgccct ttcaggcttt
    75241 catcgacatc ggtatgttcg ataatcgcta aattctctcc ggtcggcaca aagacaattt
    75301 cagcgatttg cttgccctgt tcgtcattaa tgtaaaattt attgtggcct tcgcgtattt
    75361 ccattttacc ctcgcttatt tatgacggta tttcagcgcg ccgctcgggc acgtatcaat
    75421 cactttaacc acagtgacga cgtcgacttc atccggcatg atccacggct ttcgtttgag
    75481 attaaataac ttgccgttgc cacgtacgca attgccagaa tgctgacata tcgcagtgtt
    75541 gaaatagaca tcgatctttt cgccggtata acagcgataa ccctcgtcca gtagcgcctg
    75601 atccatgact ttccctctct catcagtatg attatcgcta taagcatagc cctggaatct
    75661 tcatacagca ctggcagttt ccgttgccag agtctcttcc cctatatagc caatcattct
    75721 tactcccttt gaattacccg cctcatcaga gataatgctt aagaaatttg tcacacaagg
    75781 aagctgatga gacattcatt gccctaccgc atgttacgca aacgtccgat gaaattgagt
    75841 accacagtga tcttaatggt cagcgcggta ctgttctcgg tgctattggt ggtgcatctg
    75901 atttacttct cgcaaatcag tgatatgacg cgagacgggc tggccaacaa ggcactggca
    75961 gtggcgcgta ccctcgccga ctcgccggaa atccgtcagg gcttgcagaa aaaaccgcag
    76021 gagagcggaa tccaggccat cgcggaagcc gtgcgcaaac gcaacgatct gctgtttatt
    76081 gtcgttaccg atatgcaaag tcttcgctac tcgcatcctg aagcccagcg tattggtcag
    76141 ccatttaaag gtgatgacat ccttaaagcg ctgaatggca aagaaaatgt cgctatcaat
    76201 cgcggttttc tggcgcaggc tttacgcgta tttaccccca tctacgatga aaatcataaa
    76261 caaattggcg tggtggcgat cggccttgag ttaagccgcg tgacccaaca aatcaatgac
    76321 agtcgctgga gcattatctg gtcggtatta tttggcatgc tggtcggact gattggcacc
    76381 tgcattctgg ttaaggtact gaaaaaaatc cttttcggcc tggaacccta cgaaatctcc
    76441 acgctgtttg agcagcgcca ggccatgttg cagtctatca aagaaggcgt cgttgccgtg
    76501 gacgatcgcg gcgaggtcac gctgatcaac gatgccgcac aagaattgct gaattaccgt
    76561 aagtcgcagg acgatgagaa actgtcgacg ctcagccact catggtcaca ggtggtagat
    76621 gtctcggaag tgttacgcga cggtacgccg cgccgcgacg aagagattac gattaaagac
    76681 cggctattac tgatcaacac cgttccggtg cgcagtaatg gcgttatcat cggtgccatt
    76741 tcaaccttca gggacaaaac tgaagttcgt aaactgatgc agcgactcga cggtctggtc
    76801 aactatgctg atgcacttcg tgaacgatcc cacgaattta tgaataaatt gcatgtgatt
    76861 ctcggattat tgcatctgaa gagttataag cagttggaag attacattct caaaacagcc
    76921 aataactatc aggaagagat tggctctctg ctgggtaaga tcaaatctcc ggttatcgct
    76981 ggttttttaa tcagcaagat taaccgcgcg accgatttag gccatacgct gattttaaac
    77041 agtgaaagcc tgctgccaga cagcggcagt gaggaccagg tcgcgacgct gattaccaca
    77101 ttgggaaatc tgatagaaaa cgcgctggaa gcattagggc cggaacccgg tggcgaaatt
    77161 agcgtaacat tgcactaccg tcacggctgg ctgcactgcg aagttaacga tgatggaccg
    77221 gggatcgcac ccgacaaaat cgatcacatt tttgacaaag gtgtctcgac aaaaggaagc
    77281 gagcgaggcg tcggtttagc acttgtcaaa caacaggtag aaaatctcgg cggcagcatc
    77341 gccgtggaat cggaacccgg gattttcaca caattttttg tccagatacc ctgggacggg
    77401 gagaggtcga acagatgatc aatgtattaa ttatcgatga cgacgcaatg gtcgcggagc
    77461 tgaatcgccg atacgtagca caaatcccag gctttcaatg ctgtggaaca gcctcgacgc
    77521 tggagaaagc caaagagatt atcttcaata gcgatacgcc tatcgacctg atattgctcg
    77581 atatctatat gcaaaaagag aacgggctcg atttactgcc tgtcctgcat aacgcgcgtt
    77641 gcaaaagtga tgtgattgtc atctcctccg cagccgatgc ggcaaccatt aaagattcgc
    77701 tgcattacgg tgtcgtggat tacctgatca aacccttcca ggcttcccgc tttgaagagg
    77761 cgctcaccgg ctggcggcaa aagaaaatgg cgctggaaaa acatcagtat tatgaccagg
    77821 ccgagcttga tcagctaatc cacggcagca gctccaacga acaggatcct cgtcgcttgc
    77881 caaaaggctt aacgccgcag acgttacgca cgctgtgcca gtggattgac gcgcatcagg
    77941 actatgagtt ttcaaccgac gagctggcta acgaggttaa catctcgcgt gtttcctgcc
    78001 gaaaatacct catctggctg gtcaactgcc acatcttgtt caccagtatc cattatggcg
    78061 tcacggggcg tccggtttac cgttaccgta ttcaggcaga acactactca ctactgaaac
    78121 aatattgcca ataacggatc gctggttatc tgtaagtaat aattattacc gtaatgcaaa
    78181 atacccctgg cacaagttac atccttcatc agtaaaactt gtgccagatc aaatataatt
    78241 atccctccat catcgctaaa aattaatatc tcttcaggtg aacggtgttt ttaatttcaa
    78301 aacgctaaca aaagttaatt aactattatg tcacccgcat tatgtgtatt tttacccaca
    78361 aatgggtaga tcagattaat ctttaaacct aatgatatct gccctgagaa caaaaaatag
    78421 accgataaat atcaataaga taacagcaaa caaaacatta acatctgcgc agtacaaact
    78481 ataaacccat cgccagagag tttttctctc tgaaaaagcc gcttatcaca gtgcataaat
    78541 ttgccgctgc tttaatcagc caatatttac tgtgaggtat ttgctaaagc cggtaacgac
    78601 caaacggata tttagtcagg ctctgaaaac agttcataca aaacagaacg tgactgtgat
    78661 ctattcagca aaaatttaaa taggattatc gcgagggttc acacatgtta tttactatcc
    78721 aacttatcat aatactgata tgtctgtttt atggtgccag aaagggtggt atcgcgctgg
    78781 gtttattagg cggtatcggt ctggtcattc tggtcttcgt cttccacctt cagccaggta
    78841 aaccaccagt tgatgtcatg ctggttatca ttgcggtggt ggcggcatcg gcgaccttgc
    78901 aagcttcggg cggtcttgat gtcatgctgc aaattgccga gaagttgctg cgccgcaacc
    78961 cgaaatatgt ctcaattgtc gcgccgtttg tgacctgtac actgaccatt ctttgcggta
    79021 cgggtcatgt ggtttacacc attctgccga tcatctacga cgtcgccatt aagaacaaca
    79081 tccgtccgga acgtccgatg gcggcaagtt ctatcggtgc acagatgggg attatcgcca
    79141 gtccggtgtc ggttgcggtc gtgtctctgg ttgcaatgct gggtaatgtc acctttgatg
    79201 gtcgccatct tgagttcctc gacctgctgg caatcaccat tccatcgacg ttaatcggta
    79261 tcctggcgat cggtatcttc agctggttcc gcggtaaaga tctggataaa gacgaagagt
    79321 tccagaaatt catctccgta ccggaaaacc gtgagtatgt ttacggtgat accgcgacgc
    79381 tgctcgataa aaaactgccg aaaagcaact ggctggcaat gtggattttc ctcggtgcaa
    79441 tcgctgtagt cgcacttctt ggtgctgatt cggacctgcg tccatccttc ggcggcaaac
    79501 cgctgtcgat ggtactggtt attcagatgt ttatgctgct gaccggggcg ctgattatta
    79561 tcctgaccaa aaccaatccc gcgtctatct caaaaaacga agtcttccgt tccggtatga
    79621 tcgccatcgt ggcggtgtac ggtatcgcat ggatggcaga aaccatgttc ggtgcgcata
    79681 tgtctgaaat tcagggcgta ctgggtgaaa tggtgaaaga gtatccgtgg gcctatgcca
    79741 ttgttctgct gctggtttcc aagtttgtaa actctcaggc tgcggcgctg gcggcgattg
    79801 ttccggtcgc gctggcgatc ggcgttgatc cggcatacat cgtggcttca gcaccggctt
    79861 gctacggtta ttacatcctg ccgacttatc cgagcgatct ggcagcgatt cagtttgacc
    79921 gttccggcac cacccacatc ggtcgcttcg tcatcaacca cagctttatt ctgccggggt
    79981 tgattggtgt gagcgtatcg tgcgtcttcg gctggatctt cgccgcgatg tacgggttct
    80041 tataaatgca ctttgcgtgc cgcccgtgac tacgcggcac gccattttcg aataacaaat
    80101 acagagttac aggctggaag ctatgtcaaa caaacccttt atctaccagg cacctttccc
    80161 gatggggaaa gacaataccg aatactatct actcacttcc gattacgtta gcgttgccga
    80221 cttcgacggc gaaaccatcc tgaaagtgga accagaagcc ctgaccctgc tggcgcagca
    80281 agcctttcac gacgcttctt ttatgctccg cccggcacac cagaaacagg ttgcggctat
    80341 tcttcacgat ccagaagcca gcgaaaacga caagtacgtg gcgctgcaat tcttaagaaa
    80401 ctccgaaatc gccgccaaag gcgtgctgcc gacctgccag gataccggca ccgcgatcat
    80461 cgtcggtaaa aaaggccagc gcgtgtggac cggcggcggt gatgaagaag cgctgtcgaa
    80521 aggcgtctat aacacctata tcgaagataa cctgcgctat tcacagaatg cggcgctgga
    80581 catgtacaaa gaggtcaaca ccggcactaa cctgcctgcg caaatcgacc tgtacgcggt
    80641 agatggcgat gagtacaaat tcctttgcgt tgcgaaaggc ggcggctctg ccaacaaaac
    80701 gtatctctac caggaaacca aagccctgct gactcccggc aaactgaaaa acttcctcgt
    80761 cgagaaaatg cgtaccctcg gtactgcagc ctgcccgccg taccatatcg cgtttgtgat
    80821 tggcggtacg tctgcggaaa ccaacctgaa aaccgtcaag ttagcaagcg ctcactatta
    80881 cgatgaactg ccgacggaag gaaacgaaca tggtcaggcg ttccgcgatg tccagctgga
    80941 acaggaactg ctggaagagg cccagaaact cggtcttggc gcgcagtttg gcggtaaata
    81001 cttcgcgcac gacattcgcg ttatccgtct gccacgtcac ggcgcatcct gcccggtcgg
    81061 catgggcgtc tcctgctccg ctgaccgtaa cattaaagcg aaaatcaacc gcgaaggtat
    81121 ctggatcgaa aaactggaac acaacccagg ccagtacatt ccacaagaac tgcgccaggc
    81181 cggtgaaggc gaagcggtga aagttgacct taaccgcccg atgaaagaga tcctcgccca
    81241 gctttcgcaa tatccggttt ccactcgttt gtcgctcacc ggcaccatta tcgtgggccg
    81301 agatattgca cacgccaagc tgaaagagct gattgacgcc ggtaaagaac ttccgcagta
    81361 catcaaagat cacccgatct actacgcggg tccggcgaaa acccctgccg gttatccatc
    81421 aggttcactt ggcccaacca ccgcaggccg tatggactcc tacgtggatc tgctgcaatc
    81481 ccacggcggc agcatgatca tgctggcgaa aggtaaccgc agtcagcagg ttaccgacgc
    81541 gtgtcataaa cacggcggct tctacctcgg tagcatcggc ggtccggcgg cggtactggc
    81601 gcagcagagc atcaagcatc tggaatgcgt cgcttatccg gagctgggta tggaagctat
    81661 ctggaaaatc gaagtagaag atttcccggc gtttatcctg gtcgatgaca aaggtaacga
    81721 cttcttccag caaatcgtca acaaacagtg cgcgaactgc actaagtaac ctcttcggcc
    81781 cagcgcctgg cagcatgctg ccaggtgatc cccctgggcc acctcttttg cgattgtaat
    81841 ttcacgcttg ctggtgaata gtcagtattt tccctgactt gcgaactcac catgacccgc
    81901 acactcaagc cgttaattct taacaccggc gcactggcgc taacattaat cctgatttat
    81961 accggcattt cggcccatga caaactcacc tggctgatgg aagtgacacc ggtgattatt
    82021 gtcgtgccgc tactgcttgc caccgccaga cgttatccgt taacgccgct gctctatacg
    82081 ctcattttct ttcacgccat catcctgatg gtcggcgggc aatacaccta cgcgaaagtc
    82141 cccgttggtt ttgaggtgca ggaatggctg gggttgagtc gtaatccgta tgacaagctg
    82201 gggcactttt tccaggggct ggtgcctgca ctggtggcac gagaaattct cgttcgcggg
    82261 atgtacgtgc gcggacgtaa aatggtggcg ttcctggtct gctgcgtggc gctggcgata
    82321 agcgcgatgt atgaattaat cgagtggtgg gcagcgctgg caatgggtca gggagcggat
    82381 gattttctcg gcacccaggg cgaccagtgg gacacgcaat ccgatatgtt ctgcgcgctg
    82441 cttggcgcat taacgacggt gatattcctc gctcgctttc attgccgcca gctacggcgc
    82501 tttggcttga taaccggata acaacttgcg ctttgttgat gccggatgcg gcgtgaattc
    82561 cttatccggt ctacaaaatt tgcaaatcca aagcattaca ttaagacgtg gcctgataaa
    82621 catatcgcat caggccattt tcgttagcta ttgtccacgt caggctttca catcactcac
    82681 tgcaccgacc ggaatatcag catgaacagg ctcgggcggt actttgcgat atttatccag
    82741 cagatgaatc tggatcctgc gcagcgtgtc gccattgagg cgatagaaac ggaagtagag
    82801 aatcagcgtt accatgaaaa acagagttgg tagagcaatc ataataaact gcatacctaa
    82861 tagggcttgc gtagactgtt caacattcgg tacatagcca atcatcccta acacaaccgc
    82921 aatgaaaaaa gccgcaaagg ctgaaccgcc cttcaccacc atagtctgca cggagtaagc
    82981 gatactttca cagcgtacgt gcagtttata ttcaccgtaa tcaacggtat ctgccaccat
    83041 gatgacctgt aatacccaga aaagcgccgt tcccacattc agcaaaatac ccgcaatcac
    83101 aatgaggacg acgttgtgat agctcattaa tgccattaac aggagaacac cacagcttaa
    83161 caccggaaga atagatgctc cggcccataa aatgcgtcgg gataatgatt taactaagcg
    83221 tgggaagaat actaacgtca ccaggttagc agctcccgca tacgacagat aataggggaa
    83281 caaatccgca tcaccgataa catatgagaa ataatagata gcaaatccgg taataatgtt
    83341 gctggctaca ttataagcaa gagccatacc caagaggcat gaaagctgat cgtttttata
    83401 aattagcgca acgatggctt taagtgtcag atggcttcct tcagcagacg gttgattgtc
    83461 tgacgaaaag acttcatgca cattgcgcag agtgatgatg gttgaaacaa taaaaaaggc
    83521 gatcagtacc agagtgaaca tctgaaagcc aaatccccga tcgccaccgc cgacataatt
    83581 aacaaatggt agcgtcacac ctgccgtaac aaagcctgcc agactggcaa aaaaacgcgg
    83641 ataaggaacc agttgttcgc gctcacgttt atcgagcgtg atggttggaa ccagcgacca
    83701 gaagggaata tccataatgg tgtaagtcat gccccagagg atgtaggcca cgcaaacaaa
    83761 gacaatctga gtagtacctt caaacagatg cgcactaaag aggagaaata agattacaga
    83821 gtttgccaac gtaccgatca ggatccaggg tttgaactta ccccatcgcg atcgcgtagc
    83881 atttacaatc catcccataa tcggatcgtt aatagcatcc cagatcctcg ccaccagaaa
    83941 caaagtaccg accaaaccca cagacagccc gacgacatcg gtgtagtaat acatgaggta
    84001 catatacaca atgccgatcg caaaatcctt cccgaacgct ccaaatccat aactgagttt
    84061 tgtagtcatt gaaatgctca tagggtatcg ggtcgctgtt accaacgccc gccttctgtt
    84121 attggtatcg tcagaggtgt gcgccggaat aaatcccccg gcgcagtagc gtttagtcgc
    84181 gttttaacgg tgcaaccagc ctggcagcca gtcgccgtgg gcggcaatca ggtcgtcaac
    84241 aagagcatat atttcatcaa tgcccagcac ggcggcagta tgcgggtcca tcatcgcggc
    84301 gtggtaaaca cggtcgcgat tttccgtaag aatggcttcg gtcagcagcg tctgtacgtt
    84361 gatgttggtt tgcatcaggg cggccagatg cgaaggtagc gtaccgactt tggtcggctg
    84421 aatgccatta gcatcaacca gacaggctac ttccacgcaa catccttgtg gcaggttatc
    84481 aatcaaacca tcgttacgga cgttgccgta aatcacactc ggctcgccag tccagatagc
    84541 gttcatgatt gtgctggcat attcccgtga cggtttaata tcaatccggg aggcgttttt
    84601 atactcctcc agctctttat gccagttcgc cagctgctcg acgcagcgtt tcgggtactc
    84661 atccagcggt actttataac gctcaatcaa atcctcacga cctggcttaa taaaccacgg
    84721 tgtgtactca gcaaaatgtt ctgacgattc cgtgacgaag tagcccagct ttttgaacat
    84781 ttcgtagcgc acaatattct ggcagcgagt attaccatga atatttggct tcggtgcctg
    84841 ccctgcttca taagccgcca gcagttccgg gtagagattc acgtaactgc cgtcggcggt
    84901 tttgcgctcc agctccaggt aaaacgccat atggttgata cctgcgcagc ggtaacgcag
    84961 cgtagctggg tcgatattga gatcacgcgc cagctcttcc gccgttccct gcaccgaatg
    85021 gcacagcccg acctgtttga tatgcggata acgggcatac atcgcccagg tattcatcgc
    85081 catggggtta acatagttga gcatggtggc atcggggcag acttccgtca tgtcctcgca
    85141 aatttgccac agatgcggaa tggtacgtag cgcgcgcata ataccgcccg gccccaacgt
    85201 atcggcaatg gtttgttcca gaccatgccg cttacagacc tcgaaatcag tcaccgtgca
    85261 aggttcataa ccgccaatct gaaatgccac cacgacaaaa tcggcatcct gtaaggcttc
    85321 tttctgttgg gtgtggcagg tgattttgcc gctggcccct gctgaatcca tcagcttacg
    85381 caccacaata tgcgactctt ccaggcgggt gggatcaatg tccatcaggg caatatgcgc
    85441 cgttttcagc gcctcgcgat ggaacacatc accaagaata tttttaacga aaatcgtcga
    85501 accagcgccg ataaatgtaa ttttgggtgc agtcatcatg cagatctcct ggcttgcttg
    85561 aataacttca tcatggcagg cgaatctgca gaaaacctcc ggcttccagg ttgagcattc
    85621 ccataaactc agatcctgcg tgaagcagca gtaaatctga gtttatggta ataagttacc
    85681 tgaaaagcag agttttcggc ctcatgatgc tcggacgctc ccctcgcctc tctgggaaga
    85741 gggtgaaaac cgtgctccca ctcgcagtca tcctccctca ctcctgccat aattctgata
    85801 ttccaggaaa gagagccatc catgaataca gatacgttta tgtgcagcag cgacgaaaaa
    85861 cagacccgca gcccgctgtc gctgtattcg gaataccagc ggatggagat tgagtttcgc
    85921 gcaccgcata tcatgcccac cagccactgg catggtcagg tcgaagtgaa tgtgcctttc
    85981 gatggcgatg tggaatacct gatcaacaat gaaaaagtga atatcaatca gggtcatatc
    86041 acgctgttct gggcctgtac accgcaccaa ctaacagata ccggaacctg tcagagcatg
    86101 gcgattttta atctgccgat gcatctgttt ctctcctggc cgctggataa agacctgatt
    86161 aaccacgtca ctcacggcat ggtgatcaaa tcactggcca cacagcaact tagcccgttt
    86221 gaagtgcgcc gctggcagca ggaattaaac agtccgaacg agcaaattcg ccagctcgcc
    86281 attgatgaaa ttggcctgat gctcaagcga tttagcctct ctggctggga accgattctg
    86341 gtcaataaaa cctcgcgcac acacaaaaac agcgtctcgc gccatgcgca attttatgtt
    86401 agccagatgc tgggctttat tgccgaaaac tatgatcagg cgctgaccat caacgatgtg
    86461 gctgagcacg tcaaacttaa cgccaactat gcaatgggga tattccagcg ggtcatgcaa
    86521 ttgacgatga aacagtacat taccgcgatg cgcatcaacc acgttcgcgc gttactgagc
    86581 gataccgata aaagtattct cgatattgcc ctgacggcag gctttcgttc aagtagtcgt
    86641 ttttacagca cgttcggcaa atatgtcggc atgtcgccgc aacaataccg caaacttagc
    86701 caacagcgcc gccagacgtt tcccggctaa agacgatatc agtatcagcc aaaaaaatag
    86761 tttagccgtc gataataagc agtgaaaata atttaaaata atcacataac gtattgttta
    86821 taaaactaaa tatttcaaaa atgtttgttt ttcacgcgct ttacagcccg aaaaggccgg
    86881 aagatacttg cccgcaacga agattccttc ataaccgggt aagcaatgat gaaagtatta
    86941 attgttgaaa gcgagtttct ccatcaagac acctgggtcg gtaacgccgt tgagcgtctg
    87001 gcagatgctt taagccagca aaatgttacc gtgattaaat ccacctcctt tgatgatggt
    87061 tttgccattc tctcttcaaa cgaagccatt gactgcctga tgttcagcta tcaaatggaa
    87121 catccggacg aacatcaaaa cgtcagacaa ttgatcggta agcttcatga gcgccaacaa
    87181 aacgtgccgg tcttcctgtt gggcgatcgg gaaaaagccc tcgccgcaat ggaccgggat
    87241 ctgctggagc ttgtcgatga attcgcctgg attctggaag ataccgccga ctttatcgcc
    87301 ggacgcgccg ttgccgcgat gacccgctac cgccagcagc tgttgccgcc actgttcagc
    87361 gcgctgatga aatatagtga catccatgaa tattcctggg cagcgccagg ccaccagggc
    87421 ggcgttggtt ttaccaaaac acccgccgga cgtttctacc atgactacta tggtgaaaat
    87481 ctgtttcgca ccgacatggg catcgaacga acttccctcg gttctttgct tgaccatact
    87541 ggcgcatttg gcgaaagcga aaaatatgcc gcacgcgtat ttggtgccga tcgctcctgg
    87601 tcggtagtcg tcggtacttc cggctctaac cgcaccatca tgcaggcttg catgaccgat
    87661 aacgatgtcg tggtcgttga ccgtaattgc cataaatcca tcgaacaagg cttgatgctg
    87721 acaggcgcga aaccagtcta catggtgcca agccgcaacc gctacggcat tatcgggcca
    87781 atctatccgc aggaaatgca acctgaaacc ttgcagaaga aaatcagtga aagcccgctg
    87841 accaaagaca aagccgggca aaaaccgtct tactgcgtgg tgaccaactg cacctatgac
    87901 ggcgtgtgtt ataacgctaa agaagcgcag gatctgctgg aaaaaacctc cgatcgtctg
    87961 cactttgacg aagcctggta cggctatgca cgtttcaacc cgatctatgc cgatcactat
    88021 gccatgcgcg gcgaacctgg cgatcacaac ggtcctaccg ttttcgccac ccactccacc
    88081 cacaaactgc tgaatgcgct gtcacaggct tcttatattc atgtacgtga aggtcgtggg
    88141 gcgattaact tctcccgctt caaccaggcc tacatgatgc atgccaccac ctccccgctg
    88201 tatgccatct gcgcatccaa cgacgtggcg gtgtcgatga tggacggcaa cagcggcctg
    88261 tcactgacgc aggaagtgat cgacgaagcg gttgatttcc gtcaggcgat ggcgcggctg
    88321 tataaagagt tcaccgctga cggtagctgg ttcttcaaac cgtggaacaa agaagtcgtc
    88381 accgacccac aaaccggcaa aacctatgac tttgctgacg caccaaccaa actgctgacc
    88441 accgttcagg actgctgggt aatgcatccg ggcgaaagct ggcacggctt caaagatatt
    88501 ccggataact ggagtatgct cgacccgatt aaagtcagca tccttgctcc gggaatgggt
    88561 gaagatggtg aactggaaga aaccggtgtt ccggcggcgc tggtcactgc ctggcttggt
    88621 cgccacggca ttgtgcctac ccgcaccact gacttccaaa ttatgttcct gttctctatg
    88681 ggcgtaaccc gtgggaaatg ggggactctg gttaacaccc tttgctcctt caaacgccac
    88741 tatgacgcca acacaccgct ggcgcaggtg atgccggaac ttgttgaaca atatcctgac
    88801 acttacgcga acatggggat tcacgatctg ggtgacacca tgtttgcctg gctgaaagaa
    88861 aacaaccctg gcgcacggtt gaacgaagcc tattccggcc tgccggtggc ggaaatcacc
    88921 ccgcgtgaag cgtacaacgc gattgtcgac aacaatgtcg aactggtatc cattgaaaat
    88981 ctgccaggac gcatcgcggc aaactcagtt atcccatatc cgccaggaat cccgatgctg
    89041 ctgtctggtg aaaacttcgg cgataaaaac agtccgcaag taagttattt acgctcgctg
    89101 caatcctggg accaccattt ccctggattt gaacacgaaa ctgaagggac tgaaattatt
    89161 gacggtattt accacgttat gtgtgtgaaa gcgtaaccac tattccgctg aaggcgtaat
    89221 tgtttaaata acattacgcc gcctggcctt aggccttttg agtatggcaa cgttttcata
    89281 aaaattgctg caaacaaaaa tgtcatactt tttgcgcggc cccaccccgc gctttttttt
    89341 gcctgttatt tatcctgtaa aaaaatatgt acatgagaaa ttactataaa aatttgtaat
    89401 attagtaaaa ctcgttattt ttatgcatgt ttatattcat catacaatta tataaccatt
    89461 tcccggtatc gctttgcttt agcgagaact ggtcttttat ttgcactcag gagtacatgt
    89521 atgaggattt gcagcgacca accttgtatt gttttattga ctgaaaaaga tgtctggata
    89581 agggtgaatg ggaaagaacc tattagcctt aaagctaacc atatggcgtt attaaattgt
    89641 gaaaataata ttatcgacgt ctcctctctt aacaacactt tggttgctca tattagtcac
    89701 gacatcatca aagattacct ccggtttctg aataaagatc tctcgcaaat accagtatgg
    89761 caacgtagcg ctacgcccat actcaccctg ccatgcctga cgccagacgt ctttcgcgtt
    89821 gccgcgcaac acagcatgat gcccgcagaa actgagtcag aaaaggaacg aacacgcgca
    89881 ttattattca cggtgctatc ccgttttctc gacagtaaaa aattcctttc attaatgatg
    89941 tatatgttac gtaattgtgt aagtgacagc gtttatcaaa ttattgaaag cgatattcac
    90001 aaagactgga atcttagtat ggtagccagt tgtttatgtc ttagcccaag tctgttaaag
    90061 aaaaagctga aaagcgaaaa caccagttat agccaaataa tcaccacctg ccgcatgcgt
    90121 tatgccgtaa atgaattaat gatggacggt aaaaatatct ctcaggtatc acagtcctgc
    90181 ggctacaaca gtacgtcgta ctttatttct gtctttaaag acttctacgg tatgacgccg
    90241 ctgcattatg ttagtcagca cagagaacgc actgtcgcct gatttttaac cttaacgaag
    90301 agctatatta ataacggcat cagcgataac ccggtcgata ataattcaac tatcgaatgc
    90361 aggcgtatga tatgacgtaa tttattgtca cgaagctcgc cttcgcagga gtttaattat
    90421 gtcttcggat gctgatgctc acaaagtggg cttaatcccc gtcaccctga tggtgtcggg
    90481 gaatattatg gggtcaggtg tttttctgtt acctgcaaac ctggcctcta ctggcgggat
    90541 tgccatttat ggatggttgg tgacgattat cggtgcactg gggctctcga tggtatacgc
    90601 caaaatgtcg ttcctcgacc caagtcctgg tggttcttac gcttacgccc gccgctgctt
    90661 tggcccgttt ctcggttatc aaaccaacgt cctctactgg ctggcctgct ggatcggcaa
    90721 tatcgccatg gtggtcattg gcgtaggata tttaagttac ttcttcccga ttctgaaaga
    90781 tccattggta ttaaccatca cctgcgtcgt ggtgctgtgg atcttcgtcc tgctgaacat
    90841 tgtcggtccg aaaatgatca cccgtgtgca ggcagttgcc accgtgctgg cgctgatccc
    90901 catcgtcggg attgccgtat ttggctggtt ctggttccgt ggtgaaacct atatggcggc
    90961 atggaacgtc agcggcctgg gcaccttcgg tgcaattcaa agtaccctta acgttacgct
    91021 gtggtcgttc atcggtgtgg aaagtgcctc cgttgccgca ggtgtggtga aaaacccgaa
    91081 acgcaatgtc cctatcgcca ccattggtgg ggtattgatt gccgccgttt gctatgtact
    91141 ttctaccacc gcgattatgg ggatgatccc taatgccgca ctgcgcgttt ctgcttcgcc
    91201 attcggtgat gccgcacgga tggcgttggg tgacaccgcc ggggccattg tttccttctg
    91261 cgcagctgcg ggttgcttag gttcactggg cggctggacg ttgctggcgg gtcaaacggc
    91321 gaaagccgct gccgatgacg gactgttccc accaattttt gcccgtgtaa ataaagcggg
    91381 tacgccagtg gcggggctga ttatcgtcgg tattttgatg accatcttcc agctcagcag
    91441 catttcacca aacgcgacca aagagttcgg tctggtttct tccgtctcgg tcatctttac
    91501 actggtgcca tatctttaca cctgtgcggc gttactgctg ctcggacacg gtcactttgg
    91561 taaagcacgc ccggcatatc tggcagttac taccattgcc ttcctctact gcatctgggc
    91621 cgtggtgggg tccggagcga aagaggttat gtggtcattt gtcaccctga tggtcatcac
    91681 cgccatgtat gcgctgaatt acaaccggct acataaaaac ccgtatccct tagatgcacc
    91741 aataagcaaa gattaattcc ccttaatcca gcaaacataa aagccaacct taagaactta
    91801 aggttggctt aattttgctt tgcgagcata tgcgcacttt gttcgatgga aacaccgtga
    91861 tgttgaagcg cctactaaaa agaccctctt tgaatttact cgcctggcta ttgttggccg
    91921 ctttttatat ctctatctgc ctgaatattg ccttttttaa acaggtgttg caggcgctgc
    91981 cgctggattc gctgcataac gtactggttt tcttgtcgat gccggtcgtc gctttcagcg
    92041 tgattaatat tgtcctgaca ctaagctctt tcttatggct taatcgacca ctggcctgcc
    92101 tgtttattct ggttggcgcg gctgcacaat atttcataat gacttacggc atcgtcatcg
    92161 accgctcgat gattgccaat attattgata ccactccggc agaaagttat gcgctgatga
    92221 caccgcaaat gttattaacg ctgggattca gcggcgtgct tgctgcgctg attgcctgct
    92281 ggataaaaat caaacctgcc acctcgcgtc tgcgcagtgt tcttttccgt ggagccaata
    92341 ttctggtttc tgtactactg attttgctgg tcgccgcact gttttataaa gactacgcct
    92401 cgttgttccg caataacaaa gagctggtga aatccttaag cccctctaac agcattgttg
    92461 ccagctggtc atggtactcc catcagcgac tggcaaatct accgctggtg cgaattggtg
    92521 aagacgccca ccgcaacccg ttaatgcaga acgaaaaacg taaaaatttg accatcctga
    92581 ttgtcggcga aacctcgcgg gcggagaact tctccctcaa cggctacccg cgtgaaacta
    92641 acccgcggct ggcgaaagat aacgtggtct atttccctaa taccgcatct tgcggcacgg
    92701 caacggcagt ttcagtaccg tgcatgttct cggatatgcc gcgtgagcat tacaaagaag
    92761 agctggcaca gcaccaggaa ggcgtgctgg atatcattca gcgagcgggc atcaacgtgc
    92821 tgtggaatga caacgatggc ggctgtaaag gtgcctgcga ccgcgtgcct caccagaacg
    92881 tcaccgcgct gaatctgcct ggtcagtgca tcaacggcga atgctatgac gaagtactgt
    92941 tccacgggct ggaagagtac atcaataacc tgcagggtga tggcgtgatt gtcttacaca
    93001 ccatcggcag ccacggtccg acctattaca accgctatcc gccgcagttc aggaaattta
    93061 ccccaacctg cgacaccaat gagatccaga cctgtagcaa agagcaactg gtgaacactt
    93121 acgacaacac gctggtttac gtcgactata ttgttgataa agcgattaat ctgctgaaag
    93181 aacatcagga taaatttacc accagcctgg tttatctttc tgaccacggt gaatcgttag
    93241 gtgaaaatgg catctatctg cacggtctgc cttatgccat cgccccggat agccaaaaac
    93301 aggtgccgat gctgctgtgg ctgtcggagg attatcaaaa acggtatcag gttgaccaga
    93361 actgcctgca aaaacaggcg caaacgcaac actattcaca agacaattta ttctccacgc
    93421 tattgggatt aactggcgtt gagacgaagt attaccaggc tgcggatgat attctgcaaa
    93481 cttgcaggag agtgagtgaa tgaaaattct gattgttgaa gacgatacgc tgttattgca
    93541 gggattgatt ctggcggcgc aaactgaagg ctacgcgtgc gatggcgtga caaccgcgcg
    93601 gatggcggaa caaagccttg aggccggtca ttacagcctg gtggtactgg atttagggtt
    93661 acccgacgaa gatggactgc attttctcgc ccgtatccgg cagaaaaaat acaccctgcc
    93721 agtactgatc ctcaccgctc gcgatacgct gaccgacaaa atcgctgggc tggatgtcgg
    93781 tgccgatgac tatctggtga agccttttgc gctggaagag ttacacgccc gtatccgcgc
    93841 cctgctacga cgccataata atcagggcga aagtgagctg attgttggca atctgacgct
    93901 gaatatgggt cgccgtcagg tatggatggg cggtgaagag ttgattctga cgcccaaaga
    93961 atatgctctg ctgtcacggt taatgctcaa agcaggcagt ccggtgcatc gggaaattct
    94021 ctacaacgac atctataact gggacaatga accctcgacc aacaccctgg aagtgcatat
    94081 ccacaatctg cgcgacaaag tgggcaaagc ccgtatccgc accgtgcgcg gctttggcta
    94141 tatgctggtc gcgaatgagg aaaactaatt gaatctgatg cattttctgc gccgaccaat
    94201 atcgctgcgc caacggctga tattgaccat cggggccatt ttgttggtgt ttgagctgat
    94261 cagcgtcttc tggctatggc atgaaagtac cgagcagatt cagctgtttg agcaggcgct
    94321 acgcgacaat cgcaacaacg atcgccacat catgcgtgag atccgcgagg cggtcgccag
    94381 cctgattgtc cccggcgtct ttatggtcag cctgacgcta tttatctgct atcaggcggt
    94441 gcgccgcatc acccgcccgc tggcggagct gcaaaaagag ctggaagcgc gcactgccga
    94501 caacttaacg cccattgcca ttcacagcgc caccctcgaa atcgaagcgg tggtttcggc
    94561 gttaaacgat ctggtcagtc gcctgaccag cacgctggat aacgaaaggt tgtttaccgc
    94621 tgacgtcgcg cacgaactgc gaacgccact ggctggggtg cgtttgcatc tggaactgct
    94681 ggcgaaaacg catcacattg atgtagcacc gttagtggca cggcttgatc agatgatgga
    94741 gagcgtctcc cagctgctgc aactggcgcg tgccggacag tcattttctt ccggtaatta
    94801 tcaacatgta aaactgctgg aagatgtcat tctcccctcg tatgacgaac tcagtaccat
    94861 gctcgaccag cgacagcaaa ccctgctatt gccagagagc gccgccgaca tcaccgttca
    94921 gggtgatgca accctgctgc ggatgttatt gcgaaacctg gtagaaaacg cccatcgtta
    94981 cagcccgcaa ggcagcaaca ttatgattaa gctgcaagaa gatggcggag cggtcatggc
    95041 agttgaagat gaaggaccag gtattgatga gagtaaatgc ggggagttga gtaaagcgtt
    95101 tgtacgtatg gacagccgtt atggcgggat tggtctgggg ttaagtattg tcagccgcat
    95161 tacacagttg catcacgggc agtttttcct gcaaaaccgg caagagactt ccggcacgcg
    95221 ggcctgggta cggctgaaga aagatcagaa cgtggcaaac cagatataaa gaaagctgct
    95281 gaccaccagc acgctgaaca cggtagttaa actttcataa acacggtttt tcattgcact
    95341 ctcctccgtt aacctggagg agagtatgcg cgtcagagat taagtgaacc ttaagagttc
    95401 aattggcctg ataagacagc gtcacatcag gccatccgtt tcagcttatt catcaattcg
    95461 cggatgttgc tgcaccaggc gggtacgttt cgcctgcaaa tcggcaatct cgtggtcaat
    95521 attatcgatt ttctgctcga tattatcgta atgctcgacg agaatttcct tcgcttcctg
    95581 tatatctgac gccgccggtg tcgcaccttt caacggacga tttgccgtct ctttcatggt
    95641 tacgccggtg attaaaccaa tcaccgccac taccatcagg taataggcag gcatcatcag
    95701 attctgcgag ctttcgacca gccaggccgc cagtgttggc gtcagaccgg caaccagcac
    95761 cgaaatatta aatgccgccg ccagcgcgct gtaacggata tgcgtcggga acatcgctgg
    95821 caaggtagaa gccataacgc ccgtaaagca gttaaggatc accgccagca tcagtaaccc
    95881 ggcaaaaatc aggccgatga cgttactgtt aatcagaata aacgccggga tcgccaacac
    95941 aaacagggca acactaccaa gtagcacaaa cggacgacgg ccaaaacggt cactcagcaa
    96001 gcccatcacc ggctggacaa acagcatacc gatcataatg gcgataataa tcagcacccc
    96061 gtggtcttcg gagtaatgca ggttatgcga caaataactc ggcatatagg tcagcaacat
    96121 gtagtaagtc acgttggtgg caattaccag accaatacat gtcaacaggc tgcgccagtg
    96181 tttggaggca atctctttaa acgagacttt cgggccatcc tgcaaacctt cgcggtcgcc
    96241 ctgttccagt ttatcgacat gctgctggaa cgccggagtt tcttccagcg catggcgcag
    96301 gtaaagcccg ataatcccta acggcagagc aataaagaac ggaatacgcc agccccagtc
    96361 gaggaagttc gcttcgccga caatggtcga aattaacacc actacgccag cacccagcac
    96421 aaacccggca atagaaccaa agtccagcca gctgcccata aagccacgtt tacggtcagg
    96481 ggagtattcc gcaacaaata tcgacgcccc ggtatattca ccgccgaccg agaaaccttg
    96541 tgccatctta cagatcaaca gcagaatcgg tgcccaaata ccaatcgtgt cgtaggacgg
    96601 tattaagcca atacagaacg tactgatcga cataatcaca atagtgatag cgaggatctt
    96661 ctggcgacca tatttatcgc ccaacatacc aaagaagagt ccgccaagcg gtcgaatcag
    96721 aaagggaacg gagaacgtgg caagtgcagc aaccatctgc acgctggggt cagcccccgg
    96781 gaaaaaaact ttacctaatg cgtaagcaac aaaaccataa acaccaaaat caaaccattc
    96841 cattgcatta cccagtgatg ctgcggtaat ggctttacgc agtttaccgt catcaataat
    96901 ggtgacatca cgaagggtaa tcggttttac ttttttcctt ttcagcatag ctttcctcgc
    96961 agagttggcc catttccgca aacgccagaa aaatcagcgc gacggccgca aaaggggagc
    97021 gggaattgca ggatgcaatc tctgtaacaa gcgtagcggg tttgcttaca ccctccggtt
    97081 aaggaatgta caatcaaacc agttgacgcc tgaattgggc agttaatgac ctttttaccc
    97141 taccagtgtt taaatttgtg atcaacttca cacatggatt tcgtttagga ctcattgatg
    97201 taactgatta tttacgccaa atttacagag aagatttcct cgaccattgc ccccgccaga
    97261 cgctgcgctg aacagagtcg tggcatcccc agcgcgacag tttgccagcg ttgggtcact
    97321 gaccagcaaa cgggttgttt atctgccgtg gtccactttc ccagccaact taaaaaatca
    97381 gcatgatccc cgcccgtcgg aacggtcgaa gaggcaaacc actgatggta atagtgacgc
    97441 gtggtcgggg ccgcattgat actctctgcc agatgttgtg tcgctttcac gcgcaaatca
    97501 cccagcaaca acgccaccgc ttgcgggctt cccagacgac aggcatcgcc aaatgccccc
    97561 caacgcaact cttcgagaac gataaaacag cgcccaggca acgaccaggc gttatacgcc
    97621 ccagcacgcc agcgggtaaa aatttgctcg ctctgttcac ccgcctgcac cagtaggcct
    97681 cgttgcgtta atgccttttc cttccagcga caacgggctg aaaaccactc atacaaggcg
    97741 gcaatttgct gggggagctc cgggaaagca gagtgctggc ggtgatccat tttttgcagc
    97801 agcgccagat gctgcgcgat ctgcgtcagt gccagatgac cgggatccag catcgacgcc
    97861 agtttttcca ccaatgtcag acgcaggcta agcggcgcat ttaacatgcc gccgttcgtc
    97921 catgtctgaa gctgcacgcg cgcaagaagt tcctgcatca gtcgttcacg aaactgctgc
    97981 tgatgggctt gtggaaggcg ggcatcatcg ccctgcgcca gatccactaa aaagcgggga
    98041 taaaccgact ccagcacgcg accggggccg tcaagtaacg tcttggtcaa tatcgttctg
    98101 ccgtgaaaag tgtttgaata tcatcgcgta acagctgggc gtcggtgtaa atccagccgt
    98161 gagtcatcac agtctgctgc aattgctgct gcatcagcct gaccaccgat tcattttgtt
    98221 gacgcagagc cagactttcg cgtaaacgcg tctgtaattc cgtcaaacat gaagcgaact
    98281 cagcgaaaaa agtattcatg cctgccgtaa cagattcatc gacctgctct gccagaactt
    98341 tagccatttg ttggcaataa agatcgactt ctgcgcttaa agctcgttgc aacacactgt
    98401 aatcaaccgt ttctgtcggg gatttctcat ttccccgtcc ccagtcgggc tgattcaacc
    98461 agcgcgaaaa agtctcacgc acaacgccta aacgcgtgct ctgctcgtcc gttgcttcct
    98521 ggcgcgaaat gactt
//
LOCUS       DAEVZA010000003        84391 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEVZA010000003 DAEVZA010000000
VERSION     DAEVZA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.003"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..182)
                     /locus_tag="LAF92_000189"
     CDS             complement(<1..182)
                     /locus_tag="LAF92_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI9789254.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGT"
     gene            complement(179..1456)
                     /gene="livM"
                     /locus_tag="LAF92_000190"
     CDS             complement(179..1456)
                     /gene="livM"
                     /locus_tag="LAF92_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBI9789255.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(1453..2379)
                     /gene="livH"
                     /locus_tag="LAF92_000191"
     CDS             complement(1453..2379)
                     /gene="livH"
                     /locus_tag="LAF92_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBI9789256.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(2427..3536)
                     /gene="livK"
                     /locus_tag="LAF92_000192"
     CDS             complement(2427..3536)
                     /gene="livK"
                     /locus_tag="LAF92_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBI9789257.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            3960..4343
                     /gene="panM"
                     /locus_tag="LAF92_000193"
     CDS             3960..4343
                     /gene="panM"
                     /locus_tag="LAF92_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBI9789258.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(4542..5645)
                     /gene="livJ"
                     /locus_tag="LAF92_000194"
     CDS             complement(4542..5645)
                     /gene="livJ"
                     /locus_tag="LAF92_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBI9789259.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(5916..6770)
                     /gene="rpoH"
                     /locus_tag="LAF92_000195"
     CDS             complement(5916..6770)
                     /gene="rpoH"
                     /locus_tag="LAF92_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBI9789260.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(7015..8073)
                     /gene="ftsX"
                     /locus_tag="LAF92_000196"
     CDS             complement(7015..8073)
                     /gene="ftsX"
                     /locus_tag="LAF92_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBI9789261.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(8066..8734)
                     /gene="ftsE"
                     /locus_tag="LAF92_000197"
     CDS             complement(8066..8734)
                     /gene="ftsE"
                     /locus_tag="LAF92_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBI9789262.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(8737..10230)
                     /gene="ftsY"
                     /locus_tag="LAF92_000198"
     CDS             complement(8737..10230)
                     /gene="ftsY"
                     /locus_tag="LAF92_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBI9789263.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            10380..10976
                     /gene="rsmD"
                     /locus_tag="LAF92_000199"
     CDS             10380..10976
                     /gene="rsmD"
                     /locus_tag="LAF92_000199"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBI9789264.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            10966..11235
                     /locus_tag="LAF92_000200"
     CDS             10966..11235
                     /locus_tag="LAF92_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBI9789265.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(11238..11597)
                     /locus_tag="LAF92_000201"
     CDS             complement(11238..11597)
                     /locus_tag="LAF92_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBI9789266.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGAK
                     GTLSYKGTRFVSFVGEQ"
     gene            11738..12364
                     /locus_tag="LAF92_000202"
     CDS             11738..12364
                     /locus_tag="LAF92_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBI9789267.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            12438..14636
                     /gene="zntA"
                     /locus_tag="LAF92_000203"
     CDS             12438..14636
                     /gene="zntA"
                     /locus_tag="LAF92_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBI9789268.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTAVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(14738..14983)
                     /gene="tusA"
                     /locus_tag="LAF92_000204"
     CDS             complement(14738..14983)
                     /gene="tusA"
                     /locus_tag="LAF92_000204"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBI9789269.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            15204..15869
                     /locus_tag="LAF92_000205"
     CDS             15204..15869
                     /locus_tag="LAF92_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBI9789270.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            15942..16499
                     /gene="dcrB"
                     /locus_tag="LAF92_000206"
     CDS             15942..16499
                     /gene="dcrB"
                     /locus_tag="LAF92_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBI9789271.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16503..17720)
                     /locus_tag="LAF92_000207"
     CDS             complement(16503..17720)
                     /locus_tag="LAF92_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9789272.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            17852..18901
                     /locus_tag="LAF92_000208"
     CDS             17852..18901
                     /locus_tag="LAF92_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI9789273.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            18956..19543
                     /gene="acpT"
                     /locus_tag="LAF92_000209"
     CDS             18956..19543
                     /gene="acpT"
                     /locus_tag="LAF92_000209"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBI9789274.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            19654..21228
                     /gene="nikA"
                     /locus_tag="LAF92_000210"
     CDS             19654..21228
                     /gene="nikA"
                     /locus_tag="LAF92_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBI9789275.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            21228..22172
                     /gene="nikB"
                     /locus_tag="LAF92_000211"
     CDS             21228..22172
                     /gene="nikB"
                     /locus_tag="LAF92_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBI9789276.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            22169..23002
                     /gene="nikC"
                     /locus_tag="LAF92_000212"
     CDS             22169..23002
                     /gene="nikC"
                     /locus_tag="LAF92_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBI9789277.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            23002..23766
                     /gene="nikD"
                     /locus_tag="LAF92_000213"
     CDS             23002..23766
                     /gene="nikD"
                     /locus_tag="LAF92_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBI9789278.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            23763..24569
                     /gene="nikE"
                     /locus_tag="LAF92_000214"
     CDS             23763..24569
                     /gene="nikE"
                     /locus_tag="LAF92_000214"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBI9789279.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            24575..24976
                     /gene="nikR"
                     /locus_tag="LAF92_000215"
     CDS             24575..24976
                     /gene="nikR"
                     /locus_tag="LAF92_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBI9789280.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHELSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(25096..25455)
                     /locus_tag="LAF92_000216"
     CDS             complement(25096..25455)
                     /locus_tag="LAF92_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBI9789281.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(25452..25727)
                     /locus_tag="LAF92_000217"
     CDS             complement(25452..25727)
                     /locus_tag="LAF92_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBI9789282.1"
                     /translation="MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(25800..26924)
                     /locus_tag="LAF92_000218"
     CDS             complement(25800..26924)
                     /locus_tag="LAF92_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9789283.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(26924..29659)
                     /gene="rbbA"
                     /locus_tag="LAF92_000219"
     CDS             complement(26924..29659)
                     /gene="rbbA"
                     /locus_tag="LAF92_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBI9789284.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(29656..30723)
                     /locus_tag="LAF92_000220"
     CDS             complement(29656..30723)
                     /locus_tag="LAF92_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI9789285.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(31089..32711)
                     /locus_tag="LAF92_000221"
     CDS             complement(31089..32711)
                     /locus_tag="LAF92_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBI9789286.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ILQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(32973..34562)
                     /locus_tag="LAF92_000222"
                     /pseudo
     CDS             complement(32973..34562)
                     /locus_tag="LAF92_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            34946..35998
                     /locus_tag="LAF92_000223"
     CDS             34946..35998
                     /locus_tag="LAF92_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HBI9789287.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(36313..37515)
                     /locus_tag="LAF92_000224"
     CDS             complement(36313..37515)
                     /locus_tag="LAF92_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBI9789288.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            37747..39246
                     /gene="pitA"
                     /locus_tag="LAF92_000225"
     CDS             37747..39246
                     /gene="pitA"
                     /locus_tag="LAF92_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBI9789289.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(39317..39652)
                     /gene="uspB"
                     /locus_tag="LAF92_000226"
     CDS             complement(39317..39652)
                     /gene="uspB"
                     /locus_tag="LAF92_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBI9789290.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            40043..40477
                     /gene="uspA"
                     /locus_tag="LAF92_000227"
     CDS             40043..40477
                     /gene="uspA"
                     /locus_tag="LAF92_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBI9789291.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            40795..42264
                     /gene="dtpB"
                     /locus_tag="LAF92_000228"
     CDS             40795..42264
                     /gene="dtpB"
                     /locus_tag="LAF92_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBI9789292.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(42313..43065)
                     /gene="rsmJ"
                     /locus_tag="LAF92_000229"
     CDS             complement(42313..43065)
                     /gene="rsmJ"
                     /locus_tag="LAF92_000229"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBI9789293.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(43073..45115)
                     /gene="prlC"
                     /locus_tag="LAF92_000230"
     CDS             complement(43073..45115)
                     /gene="prlC"
                     /locus_tag="LAF92_000230"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBI9789294.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTLVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            45318..46160
                     /gene="rlmJ"
                     /locus_tag="LAF92_000231"
     CDS             45318..46160
                     /gene="rlmJ"
                     /locus_tag="LAF92_000231"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBI9789295.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            46232..47584
                     /gene="gorA"
                     /locus_tag="LAF92_000232"
     CDS             46232..47584
                     /gene="gorA"
                     /locus_tag="LAF92_000232"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBI9789296.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(47638..47721)
                     /locus_tag="LAF92_000233"
     CDS             complement(47638..47721)
                     /locus_tag="LAF92_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBI9789297.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            47749..47922
                     /locus_tag="LAF92_000234"
     CDS             47749..47922
                     /locus_tag="LAF92_000234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789298.1"
                     /translation="MHPLTHPLPVTAHALLLDNGILTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            48461..48814
                     /gene="arsR"
                     /locus_tag="LAF92_000235"
     CDS             48461..48814
                     /gene="arsR"
                     /locus_tag="LAF92_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBI9789299.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            48868..50157
                     /gene="arsB"
                     /locus_tag="LAF92_000236"
     CDS             48868..50157
                     /gene="arsB"
                     /locus_tag="LAF92_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBI9789300.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            50170..50595
                     /gene="arsC"
                     /locus_tag="LAF92_000237"
     CDS             50170..50595
                     /gene="arsC"
                     /locus_tag="LAF92_000237"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBI9789301.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(50718..51014)
                     /locus_tag="LAF92_000238"
     CDS             complement(50718..51014)
                     /locus_tag="LAF92_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9789302.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            51122..52123
                     /locus_tag="LAF92_000239"
     CDS             51122..52123
                     /locus_tag="LAF92_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI9789303.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCALAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            52704..53820
                     /locus_tag="LAF92_000240"
                     /pseudo
     CDS             52704..53820
                     /locus_tag="LAF92_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            54173..54739
                     /gene="slp"
                     /locus_tag="LAF92_000241"
     CDS             54173..54739
                     /gene="slp"
                     /locus_tag="LAF92_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBI9789304.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            54895..55425
                     /locus_tag="LAF92_000242"
     CDS             54895..55425
                     /locus_tag="LAF92_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9789305.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(55467..56114)
                     /locus_tag="LAF92_000243"
     CDS             complement(55467..56114)
                     /locus_tag="LAF92_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBI9789306.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(56178..56504)
                     /gene="hdeB"
                     /locus_tag="LAF92_000244"
     CDS             complement(56178..56504)
                     /gene="hdeB"
                     /locus_tag="LAF92_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBI9789307.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(56620..56952)
                     /gene="hdeA"
                     /locus_tag="LAF92_000245"
     CDS             complement(56620..56952)
                     /gene="hdeA"
                     /locus_tag="LAF92_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBI9789308.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            57207..57779
                     /gene="hdeD"
                     /locus_tag="LAF92_000246"
     CDS             57207..57779
                     /gene="hdeD"
                     /locus_tag="LAF92_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBI9789309.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            58578..59105
                     /gene="gadE"
                     /locus_tag="LAF92_000247"
     CDS             58578..59105
                     /gene="gadE"
                     /locus_tag="LAF92_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBI9789310.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(59106..59384)
                     /locus_tag="LAF92_000248"
     CDS             complement(59106..59384)
                     /locus_tag="LAF92_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789311.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            59444..60601
                     /gene="mdtE"
                     /locus_tag="LAF92_000249"
     CDS             59444..60601
                     /gene="mdtE"
                     /locus_tag="LAF92_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBI9789312.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            60626..63739
                     /locus_tag="LAF92_000250"
     CDS             60626..63739
                     /locus_tag="LAF92_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBI9789313.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(64102..64830)
                     /gene="gadW"
                     /locus_tag="LAF92_000251"
     CDS             complement(64102..64830)
                     /gene="gadW"
                     /locus_tag="LAF92_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBI9789314.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(65198..66022)
                     /gene="gadX"
                     /locus_tag="LAF92_000252"
     CDS             complement(65198..66022)
                     /gene="gadX"
                     /locus_tag="LAF92_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBI9789315.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            complement(66392..67792)
                     /locus_tag="LAF92_000253"
     CDS             complement(66392..67792)
                     /locus_tag="LAF92_000253"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI9789316.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            complement(68003..69400)
                     /gene="ccp"
                     /locus_tag="LAF92_000254"
     CDS             complement(68003..69400)
                     /gene="ccp"
                     /locus_tag="LAF92_000254"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBI9789317.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            69805..71454
                     /locus_tag="LAF92_000255"
     CDS             69805..71454
                     /locus_tag="LAF92_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBI9789318.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(71505..72107)
                     /locus_tag="LAF92_000256"
     CDS             complement(71505..72107)
                     /locus_tag="LAF92_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI9789319.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            72627..73526
                     /locus_tag="LAF92_000257"
     CDS             72627..73526
                     /locus_tag="LAF92_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9789320.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            73575..74588
                     /gene="yhjD"
                     /locus_tag="LAF92_000258"
     CDS             73575..74588
                     /gene="yhjD"
                     /locus_tag="LAF92_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBI9789321.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            74999..76321
                     /locus_tag="LAF92_000259"
     CDS             74999..76321
                     /locus_tag="LAF92_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBI9789322.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(76555..78615)
                     /locus_tag="LAF92_000260"
     CDS             complement(76555..78615)
                     /locus_tag="LAF92_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBI9789323.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(78685..79452)
                     /gene="pdeH"
                     /locus_tag="LAF92_000261"
     CDS             complement(78685..79452)
                     /gene="pdeH"
                     /locus_tag="LAF92_000261"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBI9789324.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVAHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            79684..80613
                     /gene="kdgK"
                     /locus_tag="LAF92_000262"
     CDS             79684..80613
                     /gene="kdgK"
                     /locus_tag="LAF92_000262"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBI9789325.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(80709..82205)
                     /locus_tag="LAF92_000263"
     CDS             complement(80709..82205)
                     /locus_tag="LAF92_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI9789326.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVNIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(82426..83712)
                     /gene="dctA"
                     /locus_tag="LAF92_000264"
     CDS             complement(82426..83712)
                     /gene="dctA"
                     /locus_tag="LAF92_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBI9789327.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(83895..>84391)
                     /locus_tag="LAF92_000265"
     CDS             complement(83895..>84391)
                     /locus_tag="LAF92_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI9789328.1"
                     /translation="LSVNLSALQLMHPNMVADMLELLTRYRIQPGTLILEVTESRRID
                     DPHAAVAILRPLRNAGVRVALDDFGMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPED
                     SSMIAAIIMLAQSLNLQMIAEGVETEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESY
                     LEEK"
BASE COUNT        20721 a        21861 c        20551 g        21258 t
ORIGIN      
        1 gtgccgccgg tgggtttgta gaatccggtc agacagttaa aaaccgtggt ttttccggca
       61 ccgttagggc cgattaacga gacgatctcc tgcgggtaca gttcaagatt gacgttgttc
      121 accgccagca ggccgccgaa gcgcatcatc aggccgttca cagataataa tggctgactc
      181 atgcctgctc tcctttcgct gcaccgtttt tcagcttcag ttgcgggcgc gtcatgggca
      241 gcaagccctg cggacgccag atcatcatca gcaccatcaa accaccgagc attaacatgc
      301 tgtattcgtt gaaatcacgc atcaattcgc gcgataccac cagcaaaatt gccgccagaa
      361 tcaccgcaaa ctgcgatccc ataccgccga gcaccactat cgccagtaca aacgccgatt
      421 cagcaaaggt gaaggattcc gggctgacaa agccctggcg cgcagcaaac agcgttccgg
      481 caaaaccggc aaacgcggcg ctgatggtaa aggcggtcag cttgatacga cgcgggctta
      541 agcccagcga acggcaggca atttcatctt cacgcaatgc ttcccacgca cgccccagcg
      601 gcatccgcag caggcggtta atgacaaaca ggcttagcac caccagcagc aacgccacca
      661 gatacaggaa gatgacgcga tcggagggat cgtatttcag gccaaagaaa ttactgaacg
      721 tgtcccagcc gccttcacga gcggtacggc tgaactcaag gccgaagaaa gtcggtttcg
      781 ggatctgact gattccgttc gggccgccgg taatttcggt gttattgagc agcaatatgc
      841 gcacaatttc gccgaaaccg agggtaacga tcgccagata gtcgccgcgc aagcgaagca
      901 ccgggaagcc gagcaggaag ccggccgccg ctgccattaa cccggcaatc ggcagacagg
      961 tccagaagcc caggccgtaa tagtgattga gcagcgcaaa agtgtaagcg ccgatggcgt
     1021 aaaagccgcc gtaccccagc accagcagac cagaaagacc aacaaccacg ttcagcccga
     1081 ggccgaggat aatgtagatc atggtcaggg tggcgatatc caccgtccca cgcgacacca
     1141 taaacggcca cgccaccgca agcaccagca gcgccacgag gaatagtttc tgcttcaccg
     1201 tggagccatc aatcgcgggc agaataaact tcggtccgga aacgcttttt agccctttct
     1261 ggaaagccgg tcgcaaaagc tggaagaaaa agaccaccgc cgtgccgata aacacccact
     1321 gccaacggat gtctgaagcc gtgtcgacca ccagtttggt gccatccagc tccagttgca
     1381 cgcccataaa gacgccagcg aggacaaaga acatcgcggc agagagcagc gccattgcaa
     1441 tatgcatcgg tttcatactt tctctacctc cgggcgaccc agaataccgg tcggcatcac
     1501 cagcagcacc agaatcagca gggcaaatga aaccacatcc ttatattccg tactcagata
     1561 ggcagaagag agcgcctccg caatccccag gatcagcccg ccaatcatcg cccctggaat
     1621 gctgccaatc ccaccgagca ccgcagcggt aaaggctttc atcccggcca taaagccgat
     1681 ataggggtta atgacgccat agaactgacc gagcagtacg ccagccactg ccgccatcgc
     1741 cgcgccaata acaaaggtca aagcaatcac ccggtcggtg ttgatgccaa gcaggctcgc
     1801 cattttcaga tcttccgcgc aggcacgaca ggcgcgcccc atacgggaat agcgaatgaa
     1861 aatcgtcagc gccagcatgg cgaggaaggt gacaatccag atcaccgcct gcatggtggt
     1921 aatagaggca gagaagtttt cgctatggcc caccacccac tgaccgttaa acaggctggg
     1981 cagcgccaca tcgcgcgaac cttcggtcag gctgacgtag ttttgcagga agatggacat
     2041 accgattgcg gaaataagcg caatcaggcg cttagaatta cgcaccgggc ggtaagccac
     2101 ccgttcgata ctccagccgt aggcgctggc aatgacgatt gcgccgacga atcccgccgc
     2161 caccagcagc cagccggtat caatgcccat catcatcagc gcggcgatga tcataaatga
     2221 gacgtagctg ccaatcatat aaacctcgcc gtgggcgaag ttgatcatgc cgataatgcc
     2281 gtaaaccatg gtgtagccga tggctatcag cgcgtaggta ctgcccagcg tgacgccgtt
     2341 aaacatctgc tgcaagaaat acaaaaactg ctcagacata aggtaacctt tctaaacccg
     2401 cccgcatttt acgggcggtg ggatgatcac ttggctgccg tggatgaacc gtcggcgtgc
     2461 cactggaaga caccaaaatc aaatccctta agatcgcctt tttcatccca gttcagcggc
     2521 ccaatcacgg tgtttgcacc gttagctttt aaatctttca ccagcgccag cggctcatcg
     2581 ctgccagtac gctcaagggc agtcgccaga gattgcaccg ccgcgtaggt gatccagaca
     2641 taaggcccgg acggatcttt cttgtctgct ttcagcgcat caacgatgcc ctggtttgcc
     2701 ggatcctggt catagcgttt tggcatagtg accaacatgc cttcggcagc atcaccggca
     2761 atattcgaca atgatgcgtt acccacacct tccggcccca taaactgagt tttcaggcca
     2821 acggaacggg cctggcgcag catctgcccc atttccgggt agtaaccgcc gtagtaaacg
     2881 aagtcgatgt tttctttttt caggcgggcg atcagcgcgg agaaatcttt ctccccggcg
     2941 gtaataccgt cgaagaagac gacgttggcg ttagccgctt tcagcccgtc ctgcaccgaa
     3001 cgcgccagcc cttcgccata ctgttgtttg tcgtgaatga tggcgatgcg ctggggcttc
     3061 accgtctcaa gaatgtattt tgccgccgtt ggcccctggg aagagtccag cccggcagta
     3121 cgcataatgt gttgataacc gcgttgggtc agctccgggt tggtcgctcc cggcgagatc
     3181 atcagaatac cttcgtcttc gtagatatct gatgcaggtt gggtagaaga agaacacaga
     3241 tgaccaataa cgtatttaat gccgtcgtta acgattttgt tggcgaccgc aacggcttgt
     3301 ttcgggtcgc aggcgtcgtc atattccacg cccaccagct tatcgccttt aattccccct
     3361 ttggcattaa tgtctttaat cgcctgacgc gcgccgttaa attccatatc gccccactgg
     3421 gcaatcgggc cggacatcgc gccgacaacg gcgactttaa tatcgtcagc catagcggtg
     3481 tgtgaaattg ccagtgcaat catccctgcg atgatagttt tcgcattccg tttcatagtc
     3541 aaaaatcccc attcgtgatg ttgtgttgct ttgtttttat gtgttaacaa atcagactgt
     3601 tcttttttta tactgcactg tttttgcctg tctgattaag ggtttagcgc agtattttgt
     3661 gataatagtg attaaaatcc ctatttttca gtcgattaag aacagataat attctgaatt
     3721 tattgataga taaacagaaa aaagtgcctt tgtcagcata aaataacggc acaaagggcg
     3781 gaataattca ctatcattca ggggattatg ctgggtattt ttcattctat aatgttttaa
     3841 ctttgtaatt attgctgtta aaaaattaat cacctgccaa aagaaataaa aaagagaaag
     3901 cctccgatta aattatttcg ctacactggt tccacttttg tgatttacac gggttaccca
     3961 tgaagctgac catcattcga ttagaaaact ttagcgacca ggaccggatt gacctgcaaa
     4021 agatctggcc ggagtattcc ccttcctctt tacaggttga cgataaccac cgcatctacg
     4081 ccgcgcgttt taacgagcgc ctgctagcgg ccgtgcgggt aaccttaagc ggcaccgagg
     4141 gagcactgga ttccctgcgc gtgcgggaag tcacccgccg tcgcggtgtg gggcaatatc
     4201 tgctggaaga ggttttgcgt aacaatcctg ccgtttcatg ctggtggatg gcggatgcag
     4261 gcgtggaaga tcgcggtgtg atgacggcgt ttatgcaggc gctggggttt acggcgcaac
     4321 agggcggctg ggagaagcgt taatcgtcaa gttgatttca aaagtgatga ccaacgagaa
     4381 attgcctgat gcgctacgct tatcaggcct acaatgtgtg ttgcaattta ctgatttctt
     4441 tggatcttgt aggccggata aggcgtttac gccgcatccg gcatgaagca acgtactcga
     4501 tattagcaat ttggcggcaa cccaaagttg ccgattaatg attacttcgc atcggtcgcc
     4561 gtgccgttgg cgtgccagtc aaatacgccg aactcaaagc ctttcagatc acctttctca
     4621 tcccacgtca gcggacccat cacggtatcc acggagttcg ctttcaggta tttggcgatc
     4681 tcagccggat cgtcagactg attcaggccc gcctgcaaag attgcagcgc ggcgtaggtg
     4741 gtccaaacga atgcgccgct tgggtcctgt tttttcgctt tgatggcgtc aacaatgggt
     4801 ttgttcgccg gaacctgatc gtagttcttc ggcttggtca ccagcagccc ttccgctgat
     4861 tcgcccgcaa tgttagacag cgaaacgtta gccacacctt ccggccccat aaactgagtt
     4921 ttcagccctg ccgcgcgtgc ctgacgcagg atttgcccca tttccgggtg ataaccgccg
     4981 tagtaaacga agtcgatatt ctcttttttc agacgggcca ccagcgttga gaaatctttt
     5041 tccccggcgg tgataccatc aaagaacacc acgttagcat tgcctttctt caggccgtcc
     5101 tgcaccgctc gcgccagacc ttcgccgtat tgctgtttgt cgtgaacgat agcaatacgc
     5161 tgcggtttca ctttctcaag aatatatttc gccgccgtcg gcccctggtc ggagtccagg
     5221 ccggtggtgc gcaggatcag ctgatagcca cgggcggtca gctccggcgc ggttgccgct
     5281 ggggtgatca ttaaaatgcc ttcgtcttcg tagatgtcag acgcaggctg cgttgatgaa
     5341 gaacagaggt gaccaatcac atatttaatg ccgtcgttaa cgactttgtt cgccaccgca
     5401 accgcctgtt tcgggtcaca ggcatcgtca tattttacga tttgcagttt gttgccttta
     5461 atgccgcctt tagcgttgat atccgcaacc gcctgctctg cgccggtaaa ctcctggtca
     5521 ccgtactgcg caaccggacc ggacattgcg cccacgaccg cgactttaat atcttctgcc
     5581 agagccatat tgctgaatgc cagcgcgata catcctgcca gtaacgcttt accctttatg
     5641 ttcatcctga gaatccccat tcttctggtt attacgtgtg ttgtggtgtt gtgattcagc
     5701 actttatttc gttttatgca tgactacccg tgctttagca gcatactctg ctaaaacata
     5761 ccccattttt atgatattgg aatagctatt ttgacagttt attaacaatc tgcgtgggga
     5821 ttgacgtttt gccggagggg gaattgatta ttgtagaggc ccaaaaaaca aaaaccccgg
     5881 actctcatcc agggttctct gcttaatagc ggaaattacg cttcaatagc ggcgcgaagt
     5941 tttttcatcg cgttcttttc cagttggcgc acacgttcag cggaaacacc gtaacggtca
     6001 gccagttcct gcaacgtgga cttgttgtct tcgtccagcc agcgcgcgcg gatgatgtcc
     6061 tggctgcgtt cgtccagacc ttgcatcgcg tcggtcagac ggtttgccgc ctgctcttcc
     6121 cagttatcat cttcgatgcc gtcggcaaag ttagatgatt tatcctgcag atagagcacc
     6181 ggtgccatcg gctggctgtc ggaatcgtcg tcggaagaca ggtcaaaggt catgtcctgt
     6241 gccgccatac gtgattccat ctcacgaacg tctttgctgg ttacgcccag ttcacgggcc
     6301 accatttcga cttcatcctg gttaaaccag cccagacgct gcttagtttt acgcaggttg
     6361 aagaacagtt tgcgctgcgc tttggtggtc gcaactttga cgatacgcca gttacgcaga
     6421 acgtattcgt ggatctctgc tttgatccag tgaacggcga aggagaccag gcgcacaccc
     6481 acttccgggt tgaaacggcg cactgctttc atcaggccga tgttaccttc ctgaatcaaa
     6541 tccgcctgtg gcaggccata gcccgcataa ttacgagcaa tatgaacaac aaaccgcagg
     6601 tgagacagga tcagcgtttt agctgcttcc agatcgccat ggtaatgcag cttttcagcc
     6661 agcgcccgct cctcgtcagc cgacaacatc ggccacgcgt tagctgcccg gatgtaggaa
     6721 tccaggttgc caactggggc taaagctaaa ctttgcattt tgtcagtcat tcaaatcctc
     6781 tcaatcgata tcttctggcg cttcagtggt agcaacaact gtgccagagc ttaagagcaa
     6841 cgaggttatc attcactgtt ttatcagacc gtgattttat ccacaagttc aatgcaagct
     6901 tgtgaataaa ttacgcacaa aatgtgacat agagatgaaa taccgggaag agacaacggg
     6961 atctctttcc ctgctacgga acccattgca gggaaagagt ataacacgct tttattattc
     7021 aggcgtaaag tggcgtaaat gttgtaccgt ggcaagccac gctgccaccc agccaatcat
     7081 cgagcatacc agcagcaata gcaggcattc atcgaacgat aaaccattga tatcaaactt
     7141 cgttccgaaa acctgtgcca cttctgcaac cgccgatgac aaacgcagta ccagaatttc
     7201 tgacaaaatt aatgacagca atgcgccaga aaatcccagc aatgcgcctc cgtacaggaa
     7261 cgggcgcagg atgaaaccat ccgtcgcacc aatcagtttc tgcacgttaa tggagtcacg
     7321 gcgagcaaag atgctcagac gcacactgtt gccgatgacg aggaacactg ctgccaccat
     7381 caacacgccg atcatcgcgg aaacgcgccc gaccagcccg gtcaacgccg ccagacgggc
     7441 aaaccagctg tcatccatcc gcacttcgtc aatgccgtta atctgggtga tacgatcacg
     7501 cagcgtattc agtgattccg tcccctgaaa atcgagtttc gggatcacca ccgccactgc
     7561 cggaagcggg ttttcttcca gcatatccag cgcaccacca aaaccagacc agttacggaa
     7621 ctcgcccagt gcgtcttcac gagaaagata gttcactttc tccacgccct gctcggcctg
     7681 tagctgtgct accacgcctg ctgcggcgtc atcgtccagc gttttttgca agtaaacagt
     7741 gatttgcggt gacggataat attgcgtcgc cgcctggtta acgtttttgt acaccatata
     7801 acagacgctg ggcagcgtca gggagatggc gataaccatc accgttaaaa acgtggcgaa
     7861 cggtttgctt ttcagatcct gcaatgcgcc gtggaaggca tagcgcacct gttcgttgaa
     7921 aacgttggtt ttgcgattta ccggttttgg cgaggatttc gcgcgttttg gcgcgttacg
     7981 accgccgtcg ccagagccgc cgaccgattt acggaagcga tccagacgcc caccaaactg
     8041 ccgaatatga ttgattgcat cgcgtttatt catggcccac gcctccatgc aagtgaccat
     8101 cgctcagggt gagcatgcga taggaacgcc gcgaaatcag gttgatgtcg tgcgttgcca
     8161 tcaatacggt taccccaacg cggttaaact cttcaaacag acgtagaatc ccttccgaca
     8221 acgcgtcgtc caggttacca gtcggttcgt ccgccagcag taccgcgggc ttattcacca
     8281 ccgcgcgggc aatgccaaca cgctgttgtt caccgccaga aagctgaata gggaagttct
     8341 tcgctttgtc cagtagcccg actttatcca gtgccgccga cacccggcga cgaatatcgt
     8401 caccgctggc accggcgata atcagcggga ttgccacgtt atcgtagaca gtacggtcca
     8461 tcagtagatg gtgatcctgg aaaatcatgc caatctggcg gcgcagaaac ggaacttcac
     8521 ggtttttcag acgcgtgatg tcatggccgc taaaccagat tttcccggcg ctgggccgct
     8581 caatcccaca gatcagcttc aggagggtac ttttccctgc gccggaatga ccggtcagaa
     8641 acgccatctc acccggctgc atatggaacg taacgccctg cagcgcctgt ctcccaccga
     8701 gataagcctt gctgacatgt tcaaagcgaa tcattgttaa tcctctcggg caaaaagtgc
     8761 ctctataaag tcgtccgcct taaacggacg caaatcctca atacgttcgc cgacaccaat
     8821 gtagcggata gggataccaa actggtcagc caccgagaaa attaccccgc ctttcgccgt
     8881 gccgtccagt ttcgttagcg tgatgccggt taagccaaca gcttcatgga acagtttggc
     8941 ctggcttacc gcgttctgcc cggtgctggc atcgatagtc agcataactt catgcggcgc
     9001 ttcaacgtcg agtttcttca tcacgcggac gattttcttc aactcttcca tcaggtgcga
     9061 tttgttctgc aggcgtccgg cggtatcggc aatcaggacg tcgatattac gcgctttagc
     9121 ggcctgaatg gcgtcgaaga taacagaggc ggaatccgca ccggtatgct gggcaatcac
     9181 cggaatattg ttgcgctgcc cccagacctg aagctgttca accgctgctg cacggaaagt
     9241 atcgcccgcc gccagcatca ccgatttacc ctgctgctca aactgacgcg ccagcttacc
     9301 aatcgtcgtg gttttaccca caccgttgac gcctaccatc aggatcacga acggcgtttt
     9361 accttcaaca ttcagcggct catcgacttt cgccagaatc tcgcccatct cttctttcag
     9421 caggccatag agggcctcgg catcacgaag ctgcttgcgg gatgcgcctt ccgtcagatt
     9481 ggtgatgatt ttacgtgtgg tttccacgcc cacatcggcg atcaacagct gttcttccag
     9541 ctcttcaaac agatcatcgt cgattttttt accgcggaac aggctgataa atccggaacc
     9601 gagattttct ttggttttta acaggctgcg tttcaggcgc gcgaagaaac cttctttggt
     9661 cggtttttcc tgctcctgag cgatttcttc caccggctgc tcttctaccg gaggaaccac
     9721 catcaccgcc tcttctgccg cttcggcagc cagcgcctgt gcttccagct cttcgtcggt
     9781 gatttcttct ttagccgctt cttcttccgc cgcttcgaca atctctacgg tttccgcttc
     9841 agcctgccac tcttctggcg aaacctcttc ggcgttgacg tcttccggca acggcagctc
     9901 ttcacgctcg atagccactg gctccggcgt ttcttctacg accggttccg gctgtgcaac
     9961 gacttccgct tcaggctgcg ctttttcact ttcagcaacc tgttcagtga cttccacaac
    10021 gtcggcagca aaagtttccg cctcggcttc agtatgcgcc tgcggctgct cttcgacggc
    10081 ttgttcagaa gccttcacag gctcttgcgc ctgaacgatt tcttctacaa ccggttgttc
    10141 attctgaact tctgtctctt tttccggggt ctgctctttt tgaccaaagc ccagccaaga
    10201 aaaaaagcca cgtttttttt ctttcgccat ttgcgactac actcctcgct gttgcttcat
    10261 ggcacagcgt taacgctatg tacatagcag ctaaaaaaat gatgaaatag tctatcactt
    10321 aacttaattc acatcaccgc ctgcaagtat gtgttatctg gcggattgag caatttatca
    10381 tgaaaaaacc gaatcattcc ggcagcggcc aaatccgcat tattggcggg cagtggcgag
    10441 gccgtaaact cccggttcct gatagcccag gtctgcgccc caccaccgac cgcgtacgcg
    10501 aaacgttgtt taactggctg gctccggtca ttgttgacgc ccaatgtctg gattgcttcg
    10561 ccgggagcgg cgcgctgggg ctggaagcgt tatcgcgcta cgctgcgggg gccacgttga
    10621 ttgagatgga tcgcgcagtt tctcagcagt taattaagaa tctggcgaca ctaaaagcag
    10681 gcaatgcacg cgtggtgaac agcaacgcaa tgtcattcct ggcgcaaaaa ggcacaccgc
    10741 ataatatcgt gtttgtcgat ccaccgttcc gccgtggctt gttagaagag acgataaatt
    10801 tactggaaga taacggctgg ctggctgacg aagccctgat ttatgtcgaa agcgaagtcg
    10861 aaaacggtct gcccactgtt ccggcaaact ggtcattaca tcgggaaaaa gtggcgggtc
    10921 aggtggctta tcggctgtat caacgcgaag cacaaggaga aagtgatgct gattaatatt
    10981 ggtcgtttgt taatgctctg cgtttgggga tttttaatcc tcaatctggt gcatcccttc
    11041 ccgcgcccgc tgaatatttt cgttaacgtg gcgctgattt ttaccgtgct gatgcatggt
    11101 atgcaactgg cgctattgaa atccacttta ccgaaagatg gcccgcagat gaccaccgcc
    11161 gaaaaggtgc ggattttcct tttcggcgtg tttgaactgc tggtctggca gaagaaattt
    11221 aaagttaaaa aataacctta ttgttcgcct acaaagctga caaagcgcgt tcctttatag
    11281 ctcagcgtac ctttagcgcc cactgtcagg gcgtggtact gctgggcgtc gaggcgaaac
    11341 gtctgctcca ttcctccgct ttgcggtttg aagcttgcct catagcgcat actggtgcct
    11401 gccggagtca cttcctgctg gcgcgaacgg cgatcgttaa tcggtttttc ccgcttgttg
    11461 ctcaccacca ccagcttttg ctggagcgga gccatatcat tatcagcttt ttcccgtcgc
    11521 tgttgcataa aacgaaacga tgcggcgacg acaattaagc caatgataac aataaagaaa
    11581 agaggtggtt tgctcatctt tatccctcat cggaaaatgc ggaaataagc ataccctgcc
    11641 agttatggtg ttgtcatccg tccaccctcg ccactaaact ggaagcaaga ccgtaggcat
    11701 tccgcttacg aaaaaataac gaattcaagg aactaagatg ctttggtcgt ttatcgctgt
    11761 ctgtctttcc gcatggctat ctgtggatgc atcgtatcgt gggccaacct ggcaacgctg
    11821 ggtgtttaaa ccgttaaccc ttcttctcct gctgttactg gcctggcaag cgccgatgtt
    11881 cgacgccatt agctatctgg tgctggcagg gctgtgcgcc tcactgctgg gcgatgcgct
    11941 aaccctgttg ccacgtcaac gtctgatgta cgccatcggc gcgtttttcc tctcgcacct
    12001 gctgtacacc atctatttcg ccagtcagat gacgctctct ttcttctggc ctctgccact
    12061 ggtgttgctg gttctgggcg cgctgttact ggcgattatc tggacgcggc tggaagagta
    12121 tcgttggcct atctgcacgt ttatcggcat gacgctggtg atggtgtggc tggcaggtga
    12181 actgtggttc ttccgtccga ccgctccggc gctctctgcg tttgtcggcg cttcgttgct
    12241 gtttatcagt aactttgtct ggctgggtag tcactatcgc cgacgcttcc gtgcggataa
    12301 cgcgattgct gcggcctgct actttgccgg tcacttcctg atcgtccgct cgctgtatct
    12361 ctgataaaac ttgactctgg agtcgactcc agagtgtatc cttcggttaa tgagaaaaaa
    12421 cttaaccgga ggatgccatg tcgactcctg acaatcacgg caagaaagcc cctcaatttg
    12481 ctgcgttcaa accgctaact gcggtacaga acgccaacga ctgttgctgc gacggcgcat
    12541 gttccagctc gccaactctc tctgaaaacg tctccggcac ccgctatagc tggaaagtca
    12601 gcggcatgga ctgcgccgcc tgtgcgcgca aggtagaaaa tgccgtgcgc cagcttgcag
    12661 gcgtgaatca ggtacaggtg ttgttcgcca ccgaaaaact ggtggttgat gccgacaatg
    12721 acatccgtgc acaagttgaa tctgcggtgc aaaaagcggg ctattccctg cgcgatgagc
    12781 aggccgccga cgagccacaa gcatcgcgcc tgaaagagaa tctgccgctg attacgctta
    12841 tcgtgatgat ggcaatcagc tggggtctgg agcaatttaa tcatccgttc gggcaactgg
    12901 cgtttatcgc gaccacgctg gttgggctgt acccgattgc tcgtcaggca ttacggctga
    12961 tcaaatccgg cagctacttc gccattgaaa ccttaatgag cgtagccgct attggtgcac
    13021 tgtttattgg cgcaacggct gaagctgcga tggtgttgct gctgtttttg attggtgaac
    13081 gactggaagg ctgggccgcc agccgcgcgc gtcagggcgt tagcgcgtta atggcgctga
    13141 aaccagaaac cgccacgcgc ctgcgtaacg gtgagcggga agaggtggcg attaacagcc
    13201 tgcggcctgg cgatgtgatt gaagtcgccg caggtgggcg tttgcctgcc gacggtaaac
    13261 tgctctcacc gtttgccagt tttgatgaaa gcgccctgac cggagaatcc attccggtgg
    13321 agcgcgcaac gggcgataaa gttcctgcag gagccaccag cgtagaccgt ctggtaacgc
    13381 tggaagtgct gtcagaaccg ggtgccagcg ccattgaccg gattctgaaa ctgatcgaag
    13441 aagccgaaga gcgccgcgcc cccattgagc ggtttatcga ccgtttcagc cgtatttata
    13501 cgccagcgat tatggccgtc gccctgctgg tgacgctggt gccaccgctg ctgtttgccg
    13561 ccagctggca ggagtggatt tataaagggc tgacgctgct gctgattggc tgcccgtgtg
    13621 cgttagttat ctccacgccc gcggcgatta cctccgggct ggcagcagcg gcacgtcgtg
    13681 gggcgttaat taaaggcgga gcggcgctgg aacagctggg tcgtgttact caggtggcgt
    13741 ttgataaaac cggtacgctg accgtcggta aaccgcgcgt taccgcgatt catccggcaa
    13801 cgggtattag tgaatctgaa ctgctgacac tggcggcggc ggtcgagcaa ggcgcgacgc
    13861 atccactggc gcaggccatc gtacgcgaag cacaggttgc tgaactcgcc attcccaccg
    13921 ccgaatcaca gcgggcgctg gtcgggtctg gcattgaagc gcaggttaac ggtgagcgcg
    13981 tattgatttg cgctgccggg aaacatcccg ctgatgcatt tgctggtttg attaatgaac
    14041 tggaaagcgc cgggcaaacg gttgtgctgg tagtacgtaa tgatgacgtg ctgggtatca
    14101 ttgcattgca ggataccctg cgcgccgacg cagcaactgc catcagtgaa ctgaacgcgc
    14161 tgggggttaa aggagtgatc ctcaccggcg ataatccacg cgcagcggcg gcaattgccg
    14221 gggagctggg gctggagttt aaagcgggcc tattgccgga agataaagtc aaagcggtga
    14281 ccaagctgaa tcaacatgcg cccctggcga tggtcggtga tggtattaac gacgcgccag
    14341 cgatgaaagc tgccgccatt gggattgcaa tgggcagtgg cacagacgtg gcgctggaaa
    14401 ccgccgacgc agcattaacc cataaccacc tgcgcggcct ggtgcaaatg attgaactgg
    14461 cacgcgccac tcacgccaat atccgccaga acatcactat tgcgctgggg ctgaaaggga
    14521 tcttcctcgt caccacgctg ctagggatga ccggactatg gctggcggtg ctggcagata
    14581 ccggtgcgac cgtgctggta acagcgaatg cgttaagatt gttgcgcagg agataaggca
    14641 aaccgatcgc aacattgagc gcgatcggtc ccctcgctcc tctggggaga gggttagggt
    14701 gaggggaaaa ggcggcatcg aagccaatct ttccccatca accgccttta cgaatcaaat
    14761 aacgataagg cagtccatcc gtctctttcg caaccagttc gtgttccata aaggtacaaa
    14821 acccaggaat atcgcgggtc gtcgccggat cgtcggcgat aatcagtaaa gtttcgccag
    14881 gctgcatatt gcgcacggtt ttgcgcacca tcatcaccgg ttccgggcag cgcaggccaa
    14941 gcgcgtcgag tgtgtggtca gggctggaaa agagatcggt cattttcttc tcatcactta
    15001 aaaaaacggc gctagtttac gccctgtgag tccgtaagca aaccaggtta acgattgcgt
    15061 gaaaattaac cattgcattg tcaacgtaaa gcagtatcat gcggcggctc gaaaaaaggg
    15121 taagcacgtt attatgttaa gataacagac gtgtcgtacg tattgggttc cctcacccca
    15181 atggttaatc aaaaaggtac aatatgaacg ttttctcgca aactcaacgc tataaggcgt
    15241 tgttctggtt atcgttattt catctgctgg tgatcacctc cagtaactat ctggtccagc
    15301 ttccggtctc catttttggt tttcatacca cctggggcgc gtttagtttt ccgtttatct
    15361 ttctcgcgac cgacctgacc gtgcgtattt ttggcgcacc gctggcccga cgcattatct
    15421 tcgcggtaat gatccctgcg ttattaatct cctatgtcat ctcgtcgctg ttctatatgg
    15481 gttcatggca gggattcggc gcactcgccc acttcaacct gtttgtcgcc cgtatcgcca
    15541 ccgccagttt catggcctac gcgctggggc aaatcctcga cgtgcacgtt tttaaccgcc
    15601 tgcgtcaaag tcgccgctgg tggctggcac cgacagcgtc cacactgttc ggtaacgtca
    15661 gcgacacgct ggcctttttc ttcattgcct tctggcgtag cccggatgcc tttatggctg
    15721 aacactggat ggaaatcgcg ctggtcgatt actgtttcaa agtgttaatc agtatcgttt
    15781 tcttcctgcc aatgtatggc gtattactca atatgctgtt gaaaagactg gcagataaat
    15841 ccgaaatcaa cgctttgcag gcgagttaaa ggttcgttat cagagttgtg ataagatgga
    15901 tgaatgagcc gttatggccg tttatcgaaa ggaagaagtc aatgcgcaat ctggttaaat
    15961 atgtcggaat tggcctgctg gttatggggc ttgcggcctg tgatgataaa gacactaacg
    16021 ctacggcgca gggttcggtc gcggaaagta acgctaccgg gaatcccgtc aacctgcttg
    16081 atggcaagtt aagtttctcg ctgccagcgg atatgaccga ccagagcggt aagctgggaa
    16141 cgcaggccaa taacatgcat gtctggtccg acgccaccgg gcagaaagca gtcatcgtca
    16201 tcatgggcga tgatccgaaa gaagatctgg cggtgctggc gaagcgtctg gaagatcagc
    16261 aacgtagccg cgatccgcag ctgcaagtgg taaccaataa agccattgag ctgaaaggtc
    16321 acaaaatgca gcagttagac agtattatct ccgcgaaagg ccagacggcg tactcttccg
    16381 ttattctggg taacgtgggt aatcaactgc tgaccatgca aattacgctg cccgctgacg
    16441 atcagcaaaa agcgcagacc accgcagaaa acatcattaa tacgctggtt attcagtaag
    16501 ttttaagatg atgaggcggc ctcagggacg tgttccggag gccgtttttt taatcgccac
    16561 gtcagtaata acgcgattgc gaccagtccc gccgccgcca gataaatcac cggtacgccc
    16621 gcccagctca tcaccagccc agccagtggt ccagtcacgc caagcgataa atccataaat
    16681 acggtgtaag ttgccagcgc cgccccctga ttttgctgcg gaaccgcttt taccgccact
    16741 acacccaatg ccgggaacac cagcgaaaac cctgcccccg ccagtaagac gccgattttc
    16801 gccatccacg gcatagtcgc cacgccaacc agtagcaggc cgattatctc aacgctaaag
    16861 caaatcatcg ctacgtttaa gccgccgata cggttaatgc cgttagggaa taacaaacgc
    16921 gtaccgacaa acgcacagct aaacagcgtc agcgcgaaag ccgcaccgtc ccaaccttta
    16981 gcgtcataaa acagcgtgat aaaggtggcg atgacgccaa atccggcgga agccagtgcc
    17041 agtgccatac cgtacagcca gacgcgccca agcaccgcgc gaaacggcag cggtttgcct
    17101 ttactggctt ttaccgtcgg acgcgggatc gccaacaaaa tggccaccag cgccacgccc
    17161 ataatgatta acgctaacgc ctgcaagccg ccccagtgat aaaacacgac gcctaacggc
    17221 gcacccatcg ccatcgcccc gtaagtgaca atgccgttcc acgaaatcac tcgcccgata
    17281 tgcagcgagc caaccacgcc aacgccccac agggtcgatc ccgttccggc aaaactttgc
    17341 ccaatcccaa ggatcacgcg tcccaggcaa agtaataaca ggctgatgac gggcagactg
    17401 gcggttaatc ctgccgtcag atatcccaga ccgctcaaaa agcagccgca taaaccgaag
    17461 acgacaatct ttttggggcc cagcaaatcg gcgtaacgtc cggcatgagg acggctcagc
    17521 aaggtggcga aatattgcag gctgataacc aatcctgccc agaaagcgct aaagcccatc
    17581 acatcatgga catagcccgg taatacagcg agcggcaacc cgatggtgag gtagctggcg
    17641 aagttaaaca tgacaatgga gacaatgcgc aaattcaggc gcaatccgtt tagcgcgggt
    17701 tcggctacgg gttcgggcat gaggatcacc acatttttac aacagtgttt catttttacc
    17761 atgtgctgac gtgaaaatca gcagtaagaa tcagaatatt gctggcgtga ctcccgctac
    17821 acttaataca aaaagtcaca aggaagcccc aatggaaacc cctcaacccg ataaaacggg
    17881 catgcacatt ctgctcaagc tggcctcgct ggtagtgatc ctcgcgggca ttcacgcagc
    17941 ggcagatatc attgtgcagc tgttactggc gctgtttttt gccatcgtcc tcaacccgct
    18001 cgttacctgg tttattcgtc ggggagtaca acgccccgtt gccattacga ttgtagtggt
    18061 ggtgatgctg atcgcactaa ccgcgctggt cggcgtactg gcggcatcgt ttaacgaatt
    18121 tatctctatg ctgccgaagt ttaataagga gctgacgcgc aaacttttta aattgcagga
    18181 gatgttgcct tttcttaatt tgcatatgtc gccggagcga atgctgcagc ggatggactc
    18241 ggaaaaagtg gttaccttca ccacagcgct aatgaccggg ctttccgggg caatggcgag
    18301 cgtgcttttg ctggtgatga ccgtagtttt tatgctgttt gaagtgcgcc acgtccctta
    18361 caaaatgcgt tttgcgctga ataatccaca gattcacatc gcgggattac accgcgcact
    18421 taaaggcgtt tcgcactatc ttgcattgaa gacgctactc agtttatgga caggtgtcat
    18481 cgtctggctg gggctggagc tgatgggggt acagtttgcg ctgatgtggg cagtactggc
    18541 gtttttgctt aactacgtgc ccaatatcgg cgcggtaatt tccgccgtac cgccaatgat
    18601 tcaggtgctg ctgtttaatg gtatttacga atgtattctg gtcggcgcat tgtttttagt
    18661 tgtccatatg gtcatcggca atattttaga accacggatg atgggccatc gcctggggat
    18721 gtccaccatg gtggtatttc tttcattgtt aatttgggga tggctgctcg gcccggtagg
    18781 gatgctactt tcggtgccat taaccagtgt gtgtaaaatc tggatggaaa ccaccaaagg
    18841 cggtagcaaa ctggcgattt tactggggcc aggcagaccg aaaagtcggt taccgggatg
    18901 aggcgacaag tgatacgata cgcactttca ttttccatta aacgttggcc ctgatatgta
    18961 tcggatagtt ctggggaaag tttcgacctt aagcgcagct ccactgccac cgggtttacg
    19021 cgagcaagca ccgcaaggtc cacgacgcga acgctggctg gcggggcgtg cattgctttc
    19081 gcacacgctt tccccgctac cggagatcat ctatggcgaa caaggcaaac ctgcattcgc
    19141 gccggaaacg ccgctatggt tcaacttaag ccatagcggt gacgatatcg ctctgctgtt
    19201 gagcgatgaa ggcgaagtcg gctgcgatat cgaagtgatt cgcccgcgcg ccaactggcg
    19261 ctggctggcg aacgccgtat tcagcctcgg ggaacacgct gagatggacg ccgtgcatcc
    19321 tgatcagcaa ctggaaatgt tctggcgcat ctggacgcgc aaagaagcca tcgttaaaca
    19381 gcgtggcggc agcgcctggc aaatcgtcag cgtagacagc acctatcact cctcgctgtc
    19441 agtcagccat tgtcagcttg aaaatttaag cctggcgatc tgcaccccca ccccctttac
    19501 gctcaccgcc gacagtgtgc aatggatcga ttcagttaac tgatccgccc acccgactgc
    19561 ccatctattg atccagaaca ggtaatcagt atgacgaata cttaaaatcg tcatacttat
    19621 ttccgccatc tattttaatc cattggggtt accatgctct ccacactccg ccgcactcta
    19681 tttgcgctgc tggcttgtgc gtcttttatc gtccatgccg ctgcaccaga tgaaatcacc
    19741 accgcctggc cggtgaatgt cgggccacta aacccgcacc tttacacgcc taaccagatg
    19801 ttcgcccaga gcatggttta tgaaccattg gtgaaatatc aggcagacgg ttcggtgatc
    19861 ccgtggctgg caaaaagctg gactcattca gaagatggta aaacctggac cttcaccctg
    19921 cgtgatgacg tgaaattctc caacggtgaa ccgttcgatg ccgaggcggc ggcagaaaac
    19981 ttccgcgcag tgctcgataa ccgtcaacgt cacgcctggc tggagctggc aaaccagatt
    20041 gttgatgtta aagcactcag taaaacagag ctgcaaatta ccctgaaaag cgcctactat
    20101 cctttcctgc aagaactggc cctgccccgt cctttccgtt ttatcgctcc ctcgcagttt
    20161 aaaaaccatg aaaccatgaa cggaattaaa gcgccgattg gcaccggacc gtggattttg
    20221 caggaatcga aactgaatca gtacgatgtc ttcgtccgta acgaaaacta ctggggcgaa
    20281 aagccagcga ttaaaaagat cacctttaac gtcatcccgg acccgactac ccgcgcggtg
    20341 gcgtttgaaa ctggcgatat cgacctgctg tacggaaacg aagggttatt accgctcgat
    20401 accttcgccc gctttagcca gaatccggct taccacaccc aactgtcaca gccgatcgaa
    20461 accgtgatgc tggcgctcaa taccgccaaa gcccccacca acgagctggc agtacgtgaa
    20521 gctcttaatt acgcggtaaa caaaaaatcg ctgattgata acgcgttata tggcacccag
    20581 caggtcgccg acaccctgtt tgccccttct gtgccttacg ctaatctcgg cctgaaaccg
    20641 cgccagtacg atccgcagaa agcgaaagag ttgctggaaa aagccggttg gacgctgcct
    20701 gcgggcaaag acatccgcga gaaaaatggt cagccgctgc gcattgaact gtctttcatc
    20761 ggcaccgatg cgttaagcaa atcgatggcg gaaatcattc aggctgatat gcgccagatt
    20821 ggcgcagatg tctcgctgat tggcgaagaa gagagcagta tctatgctcg tcagcgcgac
    20881 ggtcgttttg gcatgatttt ccaccgcacc tggggcgcgc catacgatcc acacgccttc
    20941 ctcagttcaa tgcgcgtacc gtcacacgct gacttccagg cacagcaagg attagccgac
    21001 aaaccgctga ttgataaaga gatcggcgaa gtgctggcga cccatgacga aacgcaacgt
    21061 caggcgctgt atcgcgacat tctgacccgt ctgcatgacg aggcggttta tctgcctatc
    21121 agttacatct caatgatggt ggtatcaaaa ccggagctgg gtaacatccc ctacgcgccg
    21181 atcgccactg aaattccgtt cgaacagatt aaaccggtga aaccttaatg ttgcgttacg
    21241 tattacgccg ctttctgctg ctgatcccga tggtgctcgc cgcctcggtg atcatttttc
    21301 tgatgctgcg cctcggtacc ggcgacccgg cgctcgatta tctgcgttta tctaacctgc
    21361 cgccgacgcc ggagatgctg gcctctaccc gcaccatgct cgggctggat cagccgctgt
    21421 acgtccagta cggcacctgg ttgtggaagg cgctgcatct tgactttggt atctcattcg
    21481 ccagccaacg cccggtgctg gacgatatgc tgaacttcct gcccgccacg ctggaacttg
    21541 caggtgcggc gctggtatta attctgctca cttccgtacc gctcggtatc tgggcggcgc
    21601 gccatcgcga ccgtctgccg gatttcgccg tacgtttcat cgcgtttctt ggcgtgtcga
    21661 tgcctaactt ctggctggcg tttttactgg tgatggcgtt ttcggtgtat ctgcaatggc
    21721 tacccgcgat gggttacggc ggctggcagc acatcatttt gcctgcggtt tccattgcct
    21781 ttatgtcgct ggcgattaac gcgcgtttac tgcgcgccag tatgctggac gtcgccggtc
    21841 agcgtcacgt cacctgggcg cgtctgcgcg gcctgaacga caaacagacc gaacgtcgcc
    21901 acatcctgcg caatgcctcg ctgccgatga tcaccgccgt ggggatgcac atcggcgaac
    21961 tgattggcgg gacgatgatt atcgaaaaca tctttgcctg gccgggcgtc gggcgctatg
    22021 cggtgtcggc gatttttaac cgtgactatc cggtgatcca gtgctttacg ctgatgatgg
    22081 tggtggtttt tgtggtctgt aatttgattg tcgatttgct caacgccgcg ctggacccgc
    22141 gcattcgtcg tcatgaagga gcgcacgcgt gaactttttc ctctcttccc gctggtcggt
    22201 acgcctggcg ctgatcatta tcgccctgct ggcgctgatt gcgctcacca gccagtggtg
    22261 gctgccgtat gacccccagg cgattgattt gccgtcgcgc ctgctttcgc cggatgcgca
    22321 gcactggctg ggcaccgatc acttaggtcg cgatattttc tcgcggctga tggcagcgac
    22381 ccgcgtgtcg ctcggttcgg taatggcctg cctgctgctg gtgctgacat tagggctggt
    22441 tattggcggc agcgccgggt tgattggcgg gcgcgttgat caggccacca tgcgcgtcgc
    22501 cgatatgttt atgaccttcc cgacctcgat tctgtcgttc tttatggttg gcgtgctcgg
    22561 caccgggctg accaacgtaa ttatcgccat cgccctgtcg cactgggcgt ggtatgcacg
    22621 catggtgcgc agcctggtga tttcactacg ccaacgcgag tttgtgctgg cgtcacggct
    22681 ttctggcgcg ggccatgtgc gggtgtttgt cgatcatctg gcaggcgcgg tgatcccttc
    22741 gctgctggtg ctggcaacgc tggatatcgg ccatatgatg ctgcacgtcg cggggatgtc
    22801 tttccttggc ctcggtgtga ccgcgccgac cgccgaatgg ggcgtgatga ttaacgacgc
    22861 gcgccagtat atatggaccc agccgctgca aatgttctgg ccggggctgg cgctgtttat
    22921 cagcgtgatg gcctttaacc tggtgggtga cgcactgcgc gatcatctgg accctcatct
    22981 ggtgacggag cacgcacact aatgccgcaa cagattgaac tacgtaatat cgcgctacag
    23041 gccgcgcagc cgctggtaca cggtgtatcg ttaaccctgc aacgcgggcg cgtgctggcg
    23101 ttagtcggcg gtagcggcag cgggaaatca ttaacctgcg ccgcgacgct gggcattttg
    23161 cccgctggcg ttcgccagac ggcgggggaa attttagccg atggcaaacc ggtttcgcct
    23221 tgcgccctgc gcggcatcaa aattgccacc atcatgcaga acccgcgcag cgcctttaat
    23281 ccactgcaca ccatgcacac ccacgcgcgg gaaacctgcc tggcgttagg gaaacccgcc
    23341 gatgacgcta cgcttaccgc tgccatagaa gcggtggggc tggaaaacgc cgcgcgcgtg
    23401 ctgaagctgt acccgttcga gatgagcggc ggcatgttgc agcgcatgat gattgcgatg
    23461 gcggtgctgt gtgaatcacc gtttatcatc gccgatgaac cgaccaccga cctcgacgtg
    23521 gtagcacagg cgcgcatcct cgatctgctg gaaagcatta tgcaaaaaca agcgccggga
    23581 atgctgctgg tcacccatga tatgggcgtt gtggcgcgtc tggcggatga cgtggcggtg
    23641 atgtcacaag gtaaaattgt cgaacagggc gatgtagaaa cgctgtttaa cgcccccaaa
    23701 catacggtga cgcgcagcct ggtttccgct catctcgccc tctacggtat ggagctggca
    23761 tcatgacctt acttaacgtc tgcggccttt cccatcacta tgcccacggt ggatttagcg
    23821 gaaaacatca acatcaggcg gtgctgaata acgtttccct gaccctgaaa agcggcgaaa
    23881 ccgtcgccct gctgggacgc agcggctgcg gaaaaagcac cctcgcgcgg ctgctggtag
    23941 gcttagagtc tccctcacaa gggaatatta gctggcgcgg cgaaccgctg gcgaaactca
    24001 atcgcgccca gcgtaaagcg ttccgccgcg atattcagat ggtatttcag gactccatca
    24061 gcgccgtgaa tccgcgcaaa accgtgcgcg agatcctgcg tgaaccgatg cgccacctgc
    24121 tatcactgaa aaaatccgaa caactggcgc gcgccagcga aatgctgaag gcggtcgatc
    24181 tcgatgacag cgttctcgac aaacgcccac cgcagttaag cggcggccag ctccagcgcg
    24241 tctgcctggc tcgcgcgctg gcggtcgaac cgaaactact gattctggat gaagccgttt
    24301 ctaaccttga tctcgtgtta caggcgggcg tcattcgcct gctgaaaaag ctacaacaac
    24361 agtttggcac cgcctgcctg ttcatcaccc acgacttacg cctggtggaa cgcttctgtc
    24421 agcgggtgat ggtgatggac aacggacaaa tcgtcgaaac ccaggtggtg ggagataaat
    24481 taaccttttc ctctgacgcc ggacgtgtgc tacaaaacgc ggtattaccc gcattccccg
    24541 tgcgccgtcg caccacagaa aaggtttaac gcaaatgcaa cgagtcacca tcacgcttga
    24601 tgacgattta ctggagacgc tggacagcct gagccagcgt cgtggttata acaaccgttc
    24661 cgaagctatc cgcgatattc tgcgtagcgc cctggcgcaa gaagctaccc agcagcacgg
    24721 cacacaaggt ttcgcggtgc tgtcgtatgt gtatgaacac gaaaaacgcg acttagccag
    24781 ccgtattgtt tccacccagc atcatcacca cgaactctcc gtcgccacgc tgcatgtgca
    24841 catcaaccac gacgactgtc tggaaatcgc cgtgttgaaa ggtgacatgg gtgacgtgca
    24901 gcattttgcc gatgacgtta tcgcccagcg cggcgtgcgg cacgggcatt tgcagtgctt
    24961 gccgaaggaa gattgagtta tttctgaaac gaggaaagaa aagatattaa ctgaacttaa
    25021 tttataaaat taagttcagt tgcatagata gatcctgtaa aggcagatat tctgcgtaat
    25081 gtacgccgct aaaatttata aatgattcaa acgctcattc agtgtttcaa taatataggt
    25141 attaacagag acttgttgct gcgcagccgc attattaagg cgttcactca gcgattctgg
    25201 atagcgtaac gtaaaggtct taatcttttc tgtacgcgca tacggctcaa tacccgcagc
    25261 tttacaatcc tcaagatact ctcgcaggga aagttcccct tctttttgca gcccctgaat
    25321 gctgtcagag acaaaatcac aataacctga caagcccagg aatttgccac gaaatgcatt
    25381 cagttctggc acgtaagtga taactgcagg ttgcccggca atctccatag agtttggcgt
    25441 cttcagtttg atcatggttt tacccctata cttagtaacc agtcacgcac gctttctaca
    25501 gcgcctttat cggtatctgg cgacggatga ggccgatgga aacacgcaac gctcatattt
    25561 agtatgaact tgcaacgcga acctcttcct tccttaattt ctccgcctaa tgctttaacc
    25621 agtgactcta tatcagacca cttgatccct tgaggaacag gagttttaac tatttgatcc
    25681 aatgtatttt tttgtttctt ccgtaaagcc tgtaccttgg ttctcatggc atgtccttat
    25741 gacttcactt tatgacatca atagtatgac atcacagtat gaagtcaagt ttcccttcct
    25801 tacgccattg tcccaatcgt cttcctgaat cgcagcagtg caatggtgaa aaacgcgccg
    25861 ccaattgcca tcagcgtcag gaactgcggc cagacgattt cgaatccggc accccggtag
    25921 aggatggcct gcgcgaggct aacaaagtgt gtcgtcggca tggtcagcat aatgtcctgc
    25981 accatctgcg gcatactttc gcgcggcgtg gatccaccgg aaagcatttg cagcggcagc
    26041 agcaccagaa tcaccagcag ccccagttgc ggcattgaac gcgctatcgt ccccataaaa
    26101 atgccgattg acgtggtggc aaacagactg agcgccacgc ccagcataaa cagcgggatc
    26161 gagccttcaa tcggtacacc cagcacgcct ttcaccatca gcaccagtga taatcccgac
    26221 accaccagca ccaccagccc catcgaccag acctttgcca tcatgatctc aaacggcgtt
    26281 atcggcatca ccaacaagtg ttccaccgtg ccgtgttcac gctcgcggat cagcgccgat
    26341 ccggtcaata caatcgccag catggtaatg ttgttgatga ttgccatcac cccgccaaac
    26401 cacgcgggat caaggttcgg gttaaagcgc atccgggttt ccagcgatac caacggttcg
    26461 ctgttatcac ggtagcgcgc gacaaagctg ttcacttcac cgttgataat attctggata
    26521 tacccattgc cggtaaatgc ctggctcatg cgcgtggcat cgacgttcac ctgaatatcc
    26581 ggctggcgtc cggcgaggac atcacgctga aaattaggcg gaatatttat cgcaaaggta
    26641 taacgcccgg cgtccagtcc ggcatccatc tcatcggcgg tgatcatctc cggtggcaaa
    26701 aaccacgggc gatagaagct gttaacgatc cggttcgata actgcgattg atccatatcg
    26761 gcaatggcga tcggcgcgag gttcaacgat cctggcgtaa cggtcgctga cgaatacacc
    26821 gacaccgtaa acgagaagac aatcagcgtc agcatcgctt tatcaccgag cagactgcgc
    26881 aactctttga tacccagatt aaaaatattg cgtaaatggc gcatcatccc tcctgttttt
    26941 tcagcagcag gatacttaag cccatcacca gcgggatggc tatcagcaac gggataaaaa
    27001 gttgccacaa atcagtcaga tccagcgctt tcgagaacgt tccgcgggcg atagtcagaa
    27061 aatgactggt cgggtaaacc tcgccgatcc aacgtccagg cccttccagc gaagctaccg
    27121 gatcgatcat cccggaaaac tgtgtcgccg ggatcaacgt gataatcgcc gttccgaaaa
    27181 tggcggcaat ctggcttttc ataaaggtgg agatcagcag ccccattccg gtggcaatga
    27241 tgatatacag cagcgccgcc agggtgagcg tcaggaaact gcctttatgc ggtacgccaa
    27301 acacaaacac cgacaggccg cagagcagga aaaagttcag catccccagc gcgatgtatg
    27361 gcaactgttt accaagcaaa aattcactac gcgtggtggg ggtcacgtaa aggttgataa
    27421 tcgacccaag ctctttttcc cgcaccacgc taagggcgct tagcattgac gggatcatca
    27481 tcagcagaag cgggatcacc gccggaacaa tcgctggcag gctttttacg tccgggttat
    27541 agcgatagcg cgtctcaata ttcatcagcc cgctttggct ggcgggtgtg gattgtcggc
    27601 tcgccacatc ctgtagccag ctctggtgca ttgcctgcac gtaacctttt accgtttcgg
    27661 cacggctcgg catcgctccg tcgatccaga cgccgagttc cacaggcgta ccacgcgcga
    27721 tatcgcgccc gaaattggga gggatctcaa ttgccaccgt tatatcgccc gcacgcatcc
    27781 gacgatcaag ctcgtcataa ctggtgagcg gcggctgttc gataaagtaa cgggaaccgg
    27841 agagattgag cgtccacgcc tggctactga cggtctggtc gcggtcgagc accgcaaagc
    27901 gcaggttttc cacatccata ctgatgccgt aacccattat cagcatcagg atcaccgttc
    27961 ccatcagcgc cagcgtcgaa cgtactggat cgcgtcgcag ttccagcgct tcgcggcggc
    28021 tgtagctaaa cagacggcgc aggctaaatc cctgacgcgg cgcgtgggtg gtgtcgtgta
    28081 ccacgggcgg cgcttcggct tcgttgctct gccctgccgc ttcctgcaaa taggcgataa
    28141 atgcctcttc cagactggcg gctccgcgtt tctcaaccag ttcctgcggt gtaccgctgg
    28201 caagcacttt tccggcgtgc atcagtgaga tgcggtcgca acgttccgct tcgttcatga
    28261 agtgggtgga gatgaagata gtcactttgt cctggcgcga gagatcgacc atcaactgcc
    28321 agaacatatc cctcgccacc ggatcgacac cagaagtagg ctcatcgagg attaacatct
    28381 ccgggcgatg aatcaccgcc accgccagcg aaagccgctg gcgaatgccg agcggcaatg
    28441 actccggcag aatatcttca acgtcgttga gcttaaaacg ctcgctcatt tcagccactc
    28501 ttgcgggaat ttccgcttcc gggatgtgaa acaaacgggc atgtaactca aggttttgcc
    28561 gcacggtgag ttcgttatag agcgaaaacg cctgcgacat atagcccacc cgacggcggg
    28621 tatcgatatc ttttggatca accggttgcc cgaacagcca cgcctcacct tcgctggcgg
    28681 gcagcagtcc ggtgagcatt ttcatggtgg tggatttacc gcagccgttc gaaccaagga
    28741 atccaaaaat ctccccgcgt ggaatgcgga aattaacgtg atcaacggca acgaaggaac
    28801 caaaacgcat ggtcagatcg cgcgcttcga tggcaatctc tgcgttttca ggttgatacg
    28861 gtgggatcac taccgcctga tgcgcctggc gttgcgcttg cggtaacaga tttataaatg
    28921 cttcttccag cgtagcgctt tgcgtttgct gccgtagctc ttcggcgctg ccagttgcca
    28981 gcacttctcc ggcattcatc gctaccagcc agtcgaagcg ttcggcctct tccatatagg
    29041 cggtggcgac cagcacgctc atattgctct gccgctgacg aatactgtcg atcagatccc
    29101 agaactgggc gcgggagagc gggtcaaccc ccgttgttgg ctcatcaagg atcaacagtt
    29161 ccgggtcgtg gattaacgcg cagcacagcc caagtttttg cttcatcccg ccggagagtt
    29221 tccctgccgg acgatcgcga aacggtgcta accccgtgct ggtcagcagc tcattaattc
    29281 gcacttcccg ctccgctttg tcgtgaccga acaggcgagc gaaaaaatcg acgttttcat
    29341 acaccgacaa ggtgtggtag aggtttttgc ccagcccctg cggcatccag gcgatgcgcg
    29401 ggcagacgtc gcggcgatgc ctcgggtcgc gcatatcgcc gcccagcacc atcacattgc
    29461 cctgctcaat gacgcgggca ccggaaatca acgacaataa gctcgacttc ccgacgccat
    29521 ccggaccaat cagcccgacc atacagcggg ccggaatatc gagagtaata ttgttcagcg
    29581 caacggtttt tccataatgc tggctcacgc ccgccagttg cgcgacaggc gggacgggaa
    29641 ccagttccag atgcgtcatt gcggcaacct caccacgagg tcgtcaggcc acggaagttc
    29701 ttcattcacc cgcacccacg ctacgcccgg taaaccggtt ttgacatatt ccagatgctg
    29761 ctggagtaat tccggtggga tacgcgcttt gacgcggaac atcagtttca gccgttcatc
    29821 gctggtttcg acggtttttg gcgtgaactg ggcgacactg gcgacaaaac tgatggtcgc
    29881 cggaatacgc agatctggcg cggcatcgag gatcagccgg gcttcaccac ccagtttcag
    29941 cgtgcccgcc tgttcggttg gcaggaagaa ggtcatatag acgtcgctga gatcgaccat
    30001 attcagcacc cgaccgcctg ccgccagcac ttcccctggc tcggcaaccc gatactgcac
    30061 gcgtccgtca cgcggggctt tcagttcgct gtcatcgata tctgcggcaa tgcgccgttc
    30121 agtggcttgt gccgcttcga cgcgggtttg cgcctgaatg atattggtac gtgccgcctc
    30181 tatagcggct ttagaagcag aaacctgagc ttttgccgat tccagcgcag ctcgggcgct
    30241 ctcagcggcg gcgcggtcgt catccagctg ttgcgcagaa atagcccctc gttgggccag
    30301 tgaacgggaa cgcatatgac gttttgctac ggagtccagt tctgcctggc gttgattaac
    30361 cagcgactgc gcggcgcgag tttcgctttg tcgttgctcc agcaaagcct gcgcggcggc
    30421 aacggcgctt tgtgcctctt tgatttgcgc gatggcttcc agtcgctgtt cctgcaacac
    30481 gcgagtatcc atcttcgcca gcacttcacc ttcgcgaaca aactggcctt ctttcaccag
    30541 aatggtgtcg atacgcccgg caattttgct ggcgatatcc acttccgtcg cttcaatgcg
    30601 cccattgctg acagcaaagc cttccggcac acccgccggg cgcaacagcc accacgccac
    30661 gacggcagcc accgccagtg ccccgacaac ccaccacgcc agatggcgct tactcttatc
    30721 cataatcggc ccgccataat ccctgtaagc aaacgacgcg tgttacaact gccgccgaac
    30781 gacgttcagc ggattcttcc atccgggata agaaggaaac gacaaatacg accagcgcgc
    30841 agagcattga gcgccaacgg ggaaggctta gtgaaaacgg gtagtaatgt tgtcatcatc
    30901 agcattcctg gccgtaaatg aaacacgcat actcataagt gtgatctata aaactggctg
    30961 acaaaacggt atccatcctg ttggatcgcg tttcacgcct gttcagttgc aggaacagag
    31021 cattcctttt tacgttttat caacagtctc aaccagatag taacattaat attcatgttg
    31081 tctatggttc agagctgtaa cattgtgcgc cttctaatca aaggcataag tccatttctg
    31141 tttttttcat gctggtaaga cttccaggga gtggttatct caggccctgc accagggtta
    31201 atttggcaat tatgcatgct gaacattctt ccagcatgtt tactgtcacg ggcgttaaga
    31261 cctaaaactg tccccgtttc acctatacat tctcttccac cattaccgtt atgtgcaaca
    31321 acttgattta tacctaacat ttgtgagcat gctaatagtg cttcaagttt aggtctgtaa
    31381 tctgtcgaaa cagaaaaata acgattaaag gatcgacaag agatagcccg gctattttta
    31441 aactttttgc cattaattac agcatggtgc tttttattga taattgccgc taattcgacc
    31501 ggatcgagag cattggtcat ttcatcacta gagactgtta tcggtcctat aatgtaacgt
    31561 tttcgctgat catcaaaagt tatcccatga tgatttgcca ttattttgta tttggggcca
    31621 tagaaacata caggataatc ttttatccca gcgtaggtat cctcatcaag tggtttcatg
    31681 cgcgtgaagt tctgcatata gttaccattg caattcgttt catgatttcc agctaataca
    31741 tgaacatttt cattgatact cattatgcgt tttaacaaat cgatgataaa ttgatcgcca
    31801 cagatattac taaatcgatc gccggtttgg tcgccaagaa acgtacatgg cgtcacagcg
    31861 tctttattaa taactaaata aggctgaaga ctttctaaaa ctatatctct tctgttcaga
    31921 atactgatta ttgtattaac attagtgtca agttgctcat taaagttctc ccttagcaat
    31981 acgttgagaa cttcatcttc tatattaagc aactggctaa ggaaattttc tccttcagcc
    32041 atgattctca aatgtcccgt tgcaaaaagc gcgtacaaaa ctgcaccgac tgagccatcg
    32101 gtatcgccaa aataggttgg tctggtactt gtgggatcaa caggtgaaca attggagaaa
    32161 taggttctgt cagcgaactg ggggtttatc attgtttcga atttaatatc agatacattg
    32221 aattgcaata ttttccagtc ccagacgggt ttgtacgact cagtaactgc aacatcatta
    32281 gcatcatcat ttactgtcat attattaagt aaataaacaa tgctgcacca attaatatcg
    32341 gtattatcaa gatagcagcc gtaattttta ctattaattg tcacaataaa ctgataatta
    32401 ttatccctac gtagaatatg agcgcgcatg tccgatagca tactatattt ttctgaatga
    32461 ttcgtttgta tggtaaatga tattttgtca tctcgaatag attgttggaa acgttctatt
    32521 tgccaatcat caattttttc cacaacttca ccgagcattg tcctggcaat acttgatata
    32581 tcatcaccta taccatttgc aatataccca aacagttctt tagtgaagtt agtaacatgc
    32641 tcttgcacca tatcatttgt tgcgattgtc gttgtactgt cgttagtgcc tgccacagtt
    32701 ccaattttca tcttttacct catataattc accgcgagga atgcatagtt aacatttaat
    32761 cttatgacat acacctcatg ttcgatggaa aaataattat attttgcaca aatcattcag
    32821 ccttaaagca taatatgaaa tttcttatct catagcagag aaagtcccta aagattgtag
    32881 aggcgtcatc agaacatgcc tctacaatcg attaagaaaa cctatgaaat actggcagga
    32941 ttaaaaagat ataccacagc actcatggag ttttaatgtg gttgaaaaca actaagccct
    33001 gggacacctt ttttatcacc acgttcgtta ggagctatct tccaggaatt tagccccatg
    33061 gtataattat caattttttg atgctgatct acccctaatc cataatggcc atatttctgt
    33121 ttagtttttt tgagcgtctg tccacatttg aagatatctt caggttttgg tctgaaagca
    33181 gtagtgttat taaaataatt gtcatataca ggtgtgggtg ctatagcatt gcttctaatt
    33241 agatggataa aacgatcatc catgtgttct ttatgttttt tattaaaaat attggctaat
    33301 tcctcaggat cagtaggatt tttcatctga ttaaatggaa cctgcaaagc accgagacaa
    33361 taacattttt tttcttcatc tcttattatt ccgtgatgac tggttaaaac ttgcctttct
    33421 gagtcataat tacaaacatc gagcgtttta attaagttat acgtatcacc ggcagagagt
    33481 ttatgatttg ctagtcgtgc agtatagtta ccattaaaat tgatttcatg atttccagcc
    33541 agaacgacga catttttatt aatacggcta tctttattac cctccatatt tcgcatagaa
    33601 tttagcaaag taaggatata tttatcacca aaaatagtac taaatctatc gcctgtatga
    33661 tccccaagga agatacaagg ggttatcgca tcgtccttta aaaaaacatg ttgatgcaac
    33721 tcattcaaga tcatgtctct cgtatttaat aaatcgtaga gcactttatt atttttatgt
    33781 ttgtatgcag atgaagccat gttttcgtgt tttaataatt cacataataa actccaaccc
    33841 ttttccctga tgcctatatg ccctgaaacg agcaatgcat aaagtacagc gcctactctt
    33901 ccatctgtat cgccaaaata agccgtgttg gcattttgtg ggttaatcac accacactgt
    33961 ttataaaagt ctattttaga aaacacagat ttaacatagc tatcaaactt atccttagga
    34021 aacgtctctt tagagacatc ccaacgccag tcagctttaa aattaccgct aagaagagta
    34081 aagaaatttt caggtatgat agaaaggcca ttaactttat aaattatgtc tgatttatct
    34141 ccggagaaat taataaaata ataaccatcc ttacactcaa tcgtaacggt aatgggagac
    34201 gataatttta tcacatagtt attgtctggt ataattgtaa agataaacgt gttagtagta
    34261 ttcttggcga aactattata ttcttgaata tctatagatg gctcactatt gtaaaaccat
    34321 attttctcca tctgaagact atgttcattt accctccagt gaccatgttg caggaaatag
    34381 atgatattca ggcctaaatc gttatctatt tgcgaaaagt cattcagcat attaacttaa
    34441 actgtgtttg atcgatattt tttatcaacc ttgccatggt gttgatgact gttaacttta
    34501 tctgaatgct tatcttgatg gtttgaagag aagtgcttaa atatattgtg aaatactttc
    34561 ataaataacc tgtaatattt ttacttgtta gtaacaaaat aaacaataca tgtctgaaag
    34621 acatatttac gatgatacaa agcaataata cttatatgtt cgatgatttt aagcaccgag
    34681 actatccggt cttttactaa ataatggaaa acaatccatc cctggaggat aattgtaaaa
    34741 aaaacaacag aataagaatt aatcttagga taaatattta tttatcatgg ccttttaacc
    34801 ggtaaaaaat ggcactcggc acagtaaacc ccgctatagt tatgctatgc atttgtcttt
    34861 aatgatatct cgtcgttata ttaacaggct gaaattatca ttaatgcaca tttcattatt
    34921 agcagggata cctgaggagt ttaaagtgaa catatatatc gggtggcttt tcaaattaat
    34981 ccctttgatt atgggattaa tttgcatcgc gttaggtggc tttgtgctgg aaagttcagg
    35041 gcaaagcgag tatttcgtcg cgggtcatgt gctgatttct ctggcggcca tatgcctggc
    35101 attattcact accgcattta ttatcatttc gcagctcacg cgcggcgtta atacgtttta
    35161 caatacattg ttccccatta ttggctatgc ggggtcaatt atcaccatga tatggggttg
    35221 ggcactgtta gcaggcaatg atgtgatggc agacgagttt gtcgccggcc atgttatttt
    35281 cggcgttggt atgattgccg cctgtgtatc gacggtggca gcgtcatccg gtcactttct
    35341 gctcattccc aaaaatgcag cggggagcaa gagcgatgga acgccggtac aggcttattc
    35401 ttcattaatc ggtaattgcc tcattgccgt tcccgtttta ctcaccctgc tcggtttcat
    35461 ttggtctatt acgctgttac gtagtgctga cataactccg cattatgtcg cgggtcacgt
    35521 attgcttggg ttaaccgcaa tctgtgcctg tctaattggc cttgttgcca caattgtcca
    35581 tcaaacacgt aatacgtttt caactaaaga acactggctg tggtgttatt gggttatttt
    35641 tctcggctca atcacggtac tgcaggggat atacgtctta gtcagttccg atgcaagcgc
    35701 ccgactggct cccggcatta ttcttatttg cctcggaatg atctgttaca gcatattctc
    35761 aaaagtctgg ctactggcac tggtctggag acgtacctgt tcgttagcca acagaatacc
    35821 gatgataccc gtcttcacct gcctgttttg ccttttcctg gcatcgtttc ttgcggaaat
    35881 ggcgcagacc gacatgggat attttattcc ttcgcgagtt ctggtcggtt tgggagcggt
    35941 atgctttacg ttgttctcaa tcgtttcaat attagaagcg ggttctgcta aaaaataatt
    36001 gcaacgtacc ggataaaacc agcgttgacc atttgcgtaa cgctggtttt tcttaggcat
    36061 catgaaataa cgcagcatta atgcatagtg gttaagtata aaaaacagca aagttactgt
    36121 ttttttcaac ctgttcatat ttcataaaga tctggccact gggtgagttt tcaatcacct
    36181 ttccctccac cttatattaa gcatggaggg tttcagctca cgggctcatt agaaaataat
    36241 cacaagatag ccactatcaa aattagctca tttttaatgc gactctaata attttcatct
    36301 ttaggaaata ggtcaggacg actttgctgc aatcaaatcc tgcgcacaag cccacgcact
    36361 cgaccacgcc cactggaagt tatagccccc cagccagccg gtgacgtcca tcacttcgcc
    36421 gatgaagtac agcccaggca ctttgcgcgc ttccatcgtc cgtgaagaga gttcgttggt
    36481 gtccacaccg ccgagcgtca cttcggcagt gcgatagcct tcagtgccgt tgggttgtac
    36541 gcgccagtcg gtcaatgtgc taatcagtgc ctgttggtca cgcacgttga gctgttttag
    36601 cgaaacatcc gggatttgcc cgagttgctg taagcgttca accaaccgct tcggtagatg
    36661 aaccgccagt gtgtttttca ggctctgatt cggatgtgcg ttacgctgct cattcaggaa
    36721 ggtttcgaga tccacatccg gtagcagatt gatactgaca aattcacccg gttgccagta
    36781 gctggaaatc tgcaacaccg ccggtccaga caagccgcga tgggtgaaga gtaagttctc
    36841 acggaaaacg atgccgtttt cagcggtaat cacggaaggc accgccacgc ccgccagcac
    36901 ctgtaactct tcgagcaacg gtttatgcag agtgaatggc accagacccg cgcgggtcgg
    36961 cagcacgttg aggccaaatt gttcggcaat cttataacca aacggcgacg cgcccagccc
    37021 cggcattgac agcccaccgg tcgcgatgac cagcttttcg caaccgacag tcatgccgtt
    37081 cagttcaagc gtgaagcctg tttcatcctt cgccacactc agcacttcgc tacgcaatct
    37141 gaaggttaca ttgcccttct cgcactcatc caccagcatg tcgacaatct gctgcgcgga
    37201 gtcatcgcag aagagttgtc ctaacgtttt ctcgtgccag gcgatgccgt gtttattgac
    37261 cagatcaatg aaatcccact gggtaaaacg cgcgagtgca gacttacaaa aatgcggatt
    37321 ctggctcaga taagcgcctg gttcgacata aaggttggta aagttgcagc gcccaccgcc
    37381 agacataagg attttgcgcc ctggtttttt accattatcg atcagcagaa cccggcgtcc
    37441 tgcctgacct gccagcgcag aacagaacat acccgccgca ccagcgccta taataatggc
    37501 atcaaacctt tccacgttgc gctcctctta gaaaaaacgg gcgtgaattg taaagattcc
    37561 tcagtggtcg caccagcatc aatattacta aaaggaagta tttgcctgaa ttatataaga
    37621 taattatttt ttgagtgaaa tccatacagg aggcaaatca aaaaaagtct atatttcact
    37681 ttgcccgcgc cgcgaaagtc actgataatg cgccgcgttc atgtcctcaa aatggcgtaa
    37741 cgtcctatgc tacatttgtt tgctggcctg gatttgcata ccgggctgtt attattgctt
    37801 gcactggctt ttgtgctgtt ctacgaagcc atcaatggtt tccatgacac agccaacgcc
    37861 gtggcaaccg ttatctatac ccgcgcgatg cgttctcagc tcgccgtggt tatggcggcg
    37921 gtgttcaact ttttgggtgt tttgctgggt ggtctgagtg ttgcctatgc cattgtgcat
    37981 atgctgccga cggatctgct gcttaatatg ggatcgtctc atggccttgc catggtgttc
    38041 tctatgttgc tggcggcgat tatctggaac ctgggtacct ggtactttgg tttacctgca
    38101 tccagctctc atacgctgat tggcgcgatc atcgggattg gtttaaccaa tgcgttgatg
    38161 accgggacgt cagtggtgga tgcactcaat atcccgaaag tattaagtat tttcggttct
    38221 ctgatcgttt cccctattgt cggcctggtg tttgctggcg gtctgatttt cttgctgcgt
    38281 cgctactgga gcggcaccaa gaaacgcgcc cgtatccacc tgaccccagc ggagcgtgaa
    38341 aagaaagacg gcaagaaaaa gccgccgttc tggacgcgta tcgcgctgat cctttccgct
    38401 atcggcgtgg cgttttcgca cggcgcgaac gatggtcaga aaggcattgg tctggttatg
    38461 ttggtattga ttggcgtcgc gccagcaggc ttcgtggtga acatgaatgc cactggctac
    38521 gaaatcaccc gtacccgtga tgccatcaac aacgtcgaag cttactttga gcagcatcct
    38581 gcgctgctca aacaggctac cggtgctgat cagttagtac cggctccgga agctggcgca
    38641 acgcaacctg cggagttcca ctgccatccg tcgaatacca ttaacgcgct caaccgcctg
    38701 aaaggtatgt tgaccaccga tgtggaaagc tacgacaagc tgtcgcttga tcaacgtagc
    38761 cagatgcgcc gcattatgct gtgcgtttct gacactatcg acaaagtggt gaagatgcct
    38821 ggcgtgagtg ctgacgatca gcgcctgttg aagaaactga agtctgacat gcttagcacc
    38881 atcgagtatg caccggtgtg gatcatcatg gcggtcgcgc tggcgttagg tatcggtacg
    38941 atgattggct ggcgccgtgt ggcaacgact atcggtgaga aaatcggtaa gaaaggcatg
    39001 acctacgctc aggggatgtc tgcccagatg acggcggcag tgtctatcgg cctggcgagt
    39061 tataccggga tgccggtttc cactactcac gtactctcct cttctgtcgc ggggacgatg
    39121 gtggtagatg gtggcggctt acagcgtaaa accgtgacca gcattctgat ggcctgggtg
    39181 tttacccttc cggctgcggt actgctttcc ggcgggctgt actggctctc cttgcagttc
    39241 ctgtaatcgt acacaccaaa acgagcgggt cagtttactg gcccgctttt tttatgcgtt
    39301 attattgctg aagtgcttaa tgccaaatca tcaatgcaat caggctgacc accaccagac
    39361 cacacaatgc gctggtcaga ataaactgcc gacgcacccg ctcacagcgg cgaataaatt
    39421 catcatcgtg atgatcgcga taacgttggg catagatata ccaaacgaga cgcacctgtt
    39481 tgttgggttg gccatgtgag gtaaaaaagc cccctccatc aacatattga tagagcaatg
    39541 gatcgcagtt acgcagtacc actaacaacg cgcgtagtga tgagaaatag cgcgccatgt
    39601 taacaatgca aacgacacat aaagcccaaa ataatgcgac ggtgcttatc atacctcctc
    39661 cccggcgacc tgcccgcgga gttccacccc ggggctaccg ctcccgatac gctgccaatc
    39721 agttaacacc aggtcctgga gaaaccgctt ttgtggtgac caacatccga gcggctctat
    39781 agatagtgta ggagatcagg ttgttttttt tccagaaggt taaccactat caatatattc
    39841 atgtcgaaaa tttgtttatc taacgggtaa gcaaggcgga ttgacggatc atccgggtcg
    39901 ctataaggta aggatggtct taacactgaa tctttacggc tgggttagcc ccgcgcacgt
    39961 agttcgcagg acgcgggtga cgtaacggca caagaaacgc tagctggcca gtcatcgaca
    40021 actttatgga aggagtaaca ctatggctta taaacacatt ctcatcgcag tcgatctctc
    40081 cccggaaagc aaagttctgg tagagaaagc agtctctatg gctcgcccct acaatgcgaa
    40141 agtttctctg atccacgtag atgtaaacta ctctgaccta tacaccgggc ttattgatgt
    40201 gaatctgggt gatatgcaga aacgcatctc tgaagagaca catcatgcgc tgaccgagct
    40261 ttccactaat gcaggctacc caatcactga aaccctgagc ggcagcggcg acctgggcca
    40321 ggttctggtc gatgcaatca agaaatacga tatggatctg gtggtttgtg gtcaccacca
    40381 ggacttctgg agcaaactga tgtcttccgc acgtcagctg atcaacaccg ttcacgttga
    40441 tatgctgatt gttccgctgc gcgacgaaga agaataatca tccctccacg acgtgttcct
    40501 gaacgcccgc atatgcgggc gttttgcttt ttggcgcgcc ttgttacctg atgcagcgta
    40561 aaccccttat ctggcctacg atctgcgtac gcaatcaaaa tctcctgcca atacaacatt
    40621 taacaccatc atattttcca tcattagtgt gatcagctgg ttattttctg ttgtaatagt
    40681 gtattaatct attcaccgca tcaatattaa gaatctctga cagatgtaaa ctttttcgcg
    40741 cgttatccct tacgcgttca tacttttcag gatggtattg gaaggttaat aaatatgaat
    40801 acaacaacac ccatggggat gctgcagcaa cctcgcccat ttttcatgat cttttttgtc
    40861 gagttatggg agcgattcgg ctactacggc gtgcagggcg tactggcggt tttcttcgtt
    40921 aaacagcttg gattctcgca agagcaggct tttgtcactt ttggtgcttt tgctgcgctg
    40981 gtctatggcc tcatttccat tggcggctat gtcggcgacc acctgctggg gaccaaacgc
    41041 accattgttc tcggagcact tgtgctggcg attggctact tcatgaccgg catgtcgcta
    41101 cttaagcctg acctgatttt catcgccctg gggactatcg ctgtcggtaa cggcctgttt
    41161 aaagctaacc cagccagctt gctttcgaag tgctatccgc cgaaagatcc gcggcttgat
    41221 ggcgcattca ccctgttcta tatgtcgatc aatatcggct cgttgatagc gttatcgctg
    41281 gcccctgtga tcgctgatag attcggttat tcagtcacct acaacctgtg cggggcgggg
    41341 ttaattatcg cattactggt ttacatcgcc tgtcgtggaa tggtgaaaga cattggttct
    41401 gaacccgact tcaagccgat gagcttcagc aaactgttgt acgtattact tggcagcgtg
    41461 gtgatgatct tcgtatgtgc atggctgatg cacaacgtag aagtcgccaa tctggtgctg
    41521 attgttctct ccatcgtcgt caccatcatc ttctttcgtc aggcattcaa gctggataaa
    41581 accgggcgca ataaaatgtt tgtcgccttt gtcctgatgc tcgaagcggt ggtgttttac
    41641 attctctacg cccagatgcc aacatcgctg aacttctttg ccatcaacaa cgtgcatcat
    41701 gaaattctcg gtttttccat caacccggtc agcttccagg cgcttaaccc gttctgggtg
    41761 gtactcgcca gcccaatact ggcaggcatt tacacgcatc tgggtaacaa aggcaaagac
    41821 ctctcgatgc cgatgaaatt tactctcggc atgtttatgt gctctctggg ctttttgacg
    41881 gctgcagctg cgggaatgtg gtttgcggat gcccaggggc tgacatcgcc atggtttatc
    41941 gtgctggtgt acttattcca gagcttaggt gaactgttta ttagcgccct tggcctggcg
    42001 atgattgctg ccctggtgcc gcagcatttg atgggcttta ttctcgggat gtggttcctg
    42061 acgcaggctg ccgcgttctt gctgggcggc tatgtggcaa catttaccgc ggtgccggac
    42121 aacattaccg atccgcttga gacgttgccc gtctatacca acgtgtttgg taagattggt
    42181 ctggtcacac tgggcgttgc agtagtgatg ctgttgatgg tgccgtggct gaaacgcatg
    42241 attgcgacgc cggaaagcca ttaattattc ttgcagaaag cagggtagcg ttatcgctac
    42301 cctggtttac ttttacactg gcgtacctgc ataaatatca aacctatgcc ctttagtgac
    42361 taccgcgttt ggcgtggcga cattcgccag cggtggcgcg tagtccgggc gctttaccac
    42421 cacgcgtttg gtcgccagca agcgtgcagg ctccagtaat ccatcggcat caagatccgg
    42481 ccccaccagc gactgaaaga cacgcatctc ttttttcact agcgcgcttt tctgcttatg
    42541 cgggaacatc gggtcgagat aaaccacctg cgggcgcggg gtaatatcag tcagcgccgt
    42601 caggctggag gcgtgaatta actgcaaccg ctcctgcaac cagccgccga tttccgcatc
    42661 cgcataacca cgcgccaggc cgtcgtcgag caatgcggca acgactggat tacgctccag
    42721 catccgcacg cggcagccga ctgaagccag cacaaaggca tcgcgcccca gccctgcggt
    42781 ggcatccacc acatccggca aataatcgcc tttaatgccc accgctttcg ccaccgcctc
    42841 accgcgacca ccgccgaatt tgcgtcggtg cgccatcgct ccgccaacaa aatcaacaaa
    42901 gatgccgcca agttttggct catcacgctt gcgcaattcc agatgttccg gcgttaacac
    42961 cagcgccatc aggttgtctt catcgtgctc cagcccccag cgggccgcca gaacagataa
    43021 ggcgccgtct ccggcgcctg tttcatcaat taagcagatt ttcactgaat gatcagccct
    43081 taatgccgta atgctccagc atcgcatcca gctgcggttc acgaccacgg aagcgtttga
    43141 acagatccat cggctcttct gaaccgccac ggctcagaat gttgtcgagg aacgactgcc
    43201 cggtttcacg gttgaaaatg ccctcttcct caaagcgcga gaaagcatct gccgccagta
    43261 cgtcagccca caggtagctg tagtaacctg ccgcataacc accggcgaaa atatggctga
    43321 aagcgtgcgg gaaacggccc caggacggag atggcaccac ggcaaccagt ttcttgattt
    43381 ctgccagagt ttcgaggatt ttcgctccct gatccgggcg gaactcggca tggaggcgga
    43441 aatcgaacag gccgaactcc agctggcgca gaataaacag cgccgcctgg tagttcttcg
    43501 ccgccagcat tttatccagc aactctttcg gcagcggttc gccggtttcg tagtgaccgg
    43561 agataaacgc cagcgcctcc ggctcccagc accagttttc cataaactga ctcggcagtt
    43621 cgaccgcatc ccacggcaca ccgctgatac cggaaacacc agcggtttcg atgcgggtca
    43681 gcatatggtg caggccgtga ccgaactcgt ggaacagggt gatcacttcg tcgtgagtga
    43741 acagcgccgg tttaccattt accgggcggt tgaagttaca agtcaaatac gcgaccggtt
    43801 tttgcagaga accatcagct ttacgcatct ggcctacgca gtcatccatc cacgccccgc
    43861 cgcgcttgtt ttcacgggca tacagatcga ggtagaagct gccgcgcagt tcgttatttt
    43921 cgtcatacag ttcgaagaaa cgtacatccg gatgccagac atcaacatct ttacgctctt
    43981 tagcggtgat gccgtaaata cgtttcacca cttcaaacag gccgttaacc gctttgtttt
    44041 ctgggaagta cggacgcagc tgctcgtcgc tgatgctgta gaggtgctgt ttttgttttt
    44101 cgctgtagta cgcgatatcc cacggctgca actcatcgac gccaaattcc gctttggcga
    44161 aggcgcgcag ttgtgccagc tctttttcgc cttgcggacg cgcgcgtttt gccagatcgg
    44221 ttaagaaatc cagcacctgc tgcgggtttt ctgccatttt agtggcaagg gatttaaagg
    44281 cgtagttttc aaagcccagc agttgcgcca gttcgtgacg cagagcgagg atctcttcca
    44341 tcaccttgct gttatcccat ttaccggcgt tcgggccttg atcggaggcg cgggtgctgt
    44401 aagcgcgata catctcttca cgcaaggcct ggttgtcgca gtaggtcatt accggcaggt
    44461 agcttgggat atccagcgtc agcaggtagc cttccagctc tttcgcttcg gcctgggctt
    44521 ttgccgcagc cagcgcgctt tcaggcatcc ccgccagctc cgcttcgtcg gtaacgagtt
    44581 tggtccagcc catcgtcgca tcgaggacgt tgttgctgta ctggttgccc agctcggaaa
    44641 gacgagtggc aatctcaccg tagcgttgtt gtttctctat tggcagaccg atgccagaga
    44701 gttcgaagtc gcgcagtgcg ttatcaaccg cttttttctg agccgtgttc agcgtggcgt
    44761 aatgatcgcc atcgcgcagg tcgcgatacg ctttatacag cccttcatgt tgccctacca
    44821 aggtgctgta ttccgacagc agcggcaggg tttgttcgta cgcttcacgc agttccgggc
    44881 tatttttcac cgagttcagg tggctgaccg gggagaagat acgccccagc acatcgtcca
    44941 cttccgccaa cggctggcag agattttccc aggtgtacgg tgctccttgc gctactacgc
    45001 gctccacatt ttcgcggcag tcgttcagcg ccttagtcac ggctggaacg acatgttccg
    45061 ggagaatttt agaaaacgga ggcaattcaa agggagtcag taacggattc gtcattcgcg
    45121 cagtcctggt taaagaggtt aaggaagcgc tcaacaggcg ctttacataa tgtgtgtagc
    45181 atggggttaa gtgtagtgaa tttcaatgag aaacgttacg ctttcgcggc ggcggcctct
    45241 tttcggtata ctgtcctgat acgcttttgt gcgccccgaa tacgggccga tttttactta
    45301 ccggaacacc tttacccatg ctcagttatc gccacagctt tcacgctggc aaccacgccg
    45361 acgtccttaa acataccgtt cagagcctga tcatcgagtc gctgaaagag aaagataaac
    45421 cgtttctcta tctcgacacc cacgcagggg ccgggcgtta tcagttaggc agcgaacatg
    45481 ccgagcgtac cggcgaatat ctcgaaggca tcgcccgtat ctggcagcag gacgatttgc
    45541 ccgcagaact ggaggcgtac atcaatgtgg taaaacactt caaccgtagc ggtcagttgc
    45601 gttactaccc cggttcgcca ttgattgctc gccagctact gcgcgaacaa gacagcctgc
    45661 aactgaccga actgcacccg agcgattacc cgttgctgcg ttctgaattt cagaaagaca
    45721 gccgtgcgcg cgtggaaaaa gccgatggtt tccagcagct taaggccaaa ctgccgccgg
    45781 tttcccgccg tggtttaatc cttatcgacc cgccgtatga aatgaaaacc gactatcagg
    45841 cggtggtcag cgggatagca gaaggttaca aacgtttcgc cactggtact tacgcactgt
    45901 ggtatccggt ggtgctgcgt cagcaaatta agcgcatgat ccacgatctg gaagcgaccg
    45961 gtattcgcaa aattctgcaa attgaactgg cggtactgcc agacagcgat cgccgtggca
    46021 tgaccgcttc cggcatgatt gtgattaacc cgccgtggaa actggaacag cagatgaata
    46081 acgtgctgcc gtggctgcac agcaaactgg ttccggcagg caccgggcac gccaccgtaa
    46141 gctggatcgt gccggagtaa ttgcagccat tgctggcacc tattacgtct cgcgctacaa
    46201 tcgcggtaat caacgataag gacactttgt catgactaaa cactatgatt acatcgccat
    46261 cggcggcggc agcggcggta tcgcctccat caaccgcgcg gctatgtacg gccagaaatg
    46321 tgcgctgatt gaagccaaag agctgggcgg cacctgcgta aatgttggct gtgtgccgaa
    46381 aaaagtgatg tggcacgcgg cgcaaatccg tgaagcgatc catatgtacg gcccggatta
    46441 tggttttgat accactatca ataaattcaa ctgggaaacg ttgatcgcca gccgtaccgc
    46501 ctatatcgac cgtattcata cttcctatga aaacgtgctc ggtaaaaata acgttgatgt
    46561 aatcaaaggc tttgcccgct tcgttgatgc caaaacgctg gaggtaaacg gcgaaaccat
    46621 cacggccgat catattctga tcgccacagg cggtcgtccg agccacccgg atattccggg
    46681 cgtggaatac ggtattgatt ctgatggctt cttcgccctt cctgctttgc cagagcgcgt
    46741 ggcggttgtt ggcgcgggtt acatcgccgt tgagctggcg ggcgtgatta acggcctcgg
    46801 cgcgaaaacg catctgtttg tgcgtaaaca tgcgccgctg cgcagcttcg acccgatgat
    46861 ttccgaaacg ctggtcgaag tgatgaacgc cgaaggcccg cagctgcaca ccaacgccat
    46921 cccgaaagcg gtagtgaaaa atgccgatgg tagcctgacg ctggagctgg aagatggtcg
    46981 cagtgaaacg gtggattgcc tgatttgggc gattggtcgc gagcctgcca atgacaacat
    47041 caacctggaa gccgctggcg ttaaaactaa cgaaaaaggc tatatcgtcg tcgataaata
    47101 tcaaaacacc aatattgaag gtatttacgc ggtgggcgat aacacgggtg cagtggagct
    47161 gacaccggtg gcagttgcag cgggtcgccg tctctctgaa cgcctgttta ataacaagcc
    47221 ggatgagcat ctggattaca gcaacattcc gaccgtggtc ttcagccatc cgccgattgg
    47281 tactgttggt ttaacggaac cgcaggcgcg cgagcagtat ggcgacgatc aggtgaaagt
    47341 gtataaatcc tctttcaccg cgatgtatac cgccgtcacc actcaccgcc agccgtgccg
    47401 catgaagctg gtgtgcgttg gatcggaaga gaagattgtc ggtattcacg gcattggctt
    47461 tggtatggac gaaatgttgc agggcttcgc ggtggcgctg aagatgggtg caaccaaaaa
    47521 agacttcgac aataccgtcg ccattcaccc aacggcggca gaagagttcg tgacaatgcg
    47581 ttaaatgtta aagggctaag agtagtgtgc tcttagccct taattacgtt tccgctatca
    47641 gttcagaagc tgaagcagaa agcggatcag ttccagcagc gcaattaacg ccccaagaac
    47701 gatgattgct ttatcaatca cccgttttct ccatgcgatg gagtgagaat gcatccgctt
    47761 actcatccac tgcctgtcac ggcacatgcc ctattgttag ataatggcat tctcactccg
    47821 gccagagcat cagttaacgg caccacccgt acttctgacc aggactttga aagcgtttat
    47881 gcgcattacc agagtgaaaa tgcctcagag ctaactggat aatcatacag tacattcagg
    47941 ttataaaacc agcatgtcct tgcaatagtt tcagtatggt attagcattg atgcgttaga
    48001 taatggcatt ctcactccag ccagtgccac caactcaggg ctggaaagta aaaaaccgac
    48061 gcaaagtcgg tttttttaca tccggattcg gacaaggctt aatatgacga tgacccagtg
    48121 aaagtatata aatcgtcatt gcgatatata ccgaagtgcc ccctccgcca gctgaagaaa
    48181 tcgctaattc ttgcaatgtt agccactggc taatagtatt gagctgttag ataagaactc
    48241 tctcactcca gccagagcca ccaactcagg gctggaaagt aaaaaaccga cgcaaagtcg
    48301 gtttttttac gtcctgattc agacctcctt tcaaatgaat agccaactca aaattcacac
    48361 ctattacctt cctctgcact tacacattcg ttaagtcata tatgtttttg acttatccgc
    48421 ttcgaagaga gacactacct gcaacaatca ggagcgcaat atgtcatttc tgttacccat
    48481 ccaattgttc aaaattcttg ctgatgaaac ccgtctgggc atcgttttac tgctcagcga
    48541 actgggagag ttatgcgtct gcgatctctg cactgctctc gaccagtcgc agcccaagat
    48601 ctcccgccac ctggcattgc tgcgtgaaag cgggctgttg ctggaccgca agcaaggtaa
    48661 gtgggttcat taccgcttat caccgcatat tccagcatgg gcggcgaaaa ttattgatga
    48721 ggcctggcga tgtgaacagg aaaaggttca ggcgattgtc cgcaacctgg ctcgacaaaa
    48781 ctgttccggg gacagtaaga acatttgcag ttaaaaattt agctaaacac atatgaattt
    48841 tcagatgtgt tttatccggg aggcattatg ttactggcag gcgctatctt tgtcctgacc
    48901 atcgtattgg ttatctggca gccgaaaggt ttaggcatcg gctggagtgc aacgctcggc
    48961 gcagtactgg cgttagttac gggcgtggtc catccgggtg atattccggt ggtgtggaat
    49021 atcgtctgga acgcgacggc tgcgtttatc gccgtcatta tcatcagcct gctgctggat
    49081 gagtccggct tttttgaatg ggcggcgctg cacgtctcac gctggggtaa tggtcgtggt
    49141 cgcttgctgt ttacctggat tgtcctgctc ggtgctgccg ttgccgccct gtttgccaat
    49201 gatggcgcgg cgcttatttt gacaccgatt gtcatcgcca tgctgctggc tttagggttc
    49261 agtaaaggca ctacgctggc gttcgtgatg gcggccggat tcattgccga taccgccagc
    49321 ctgccgctta ttgtctccaa cctggtgaat atcgtttccg ctgatttctt tggcctcggc
    49381 tttcgcgaat acgcctcggt gatggtgccg gtggatatcg ccgcgattgt tgccacgctg
    49441 gtgatgttac atctctattt tcgcaaagat attccgcaga actacgatat ggcgctgctg
    49501 aaatctcccg cagaagcgat caaagatcct gctacgttca aaactggctg ggttgtttta
    49561 ctgcttctgc tggtgggatt tttcgtcctg gaaccgctcg gcattccggt gagcgccatt
    49621 gcagctgtgg gcgcgctgat attatttgtc gtcgctaaac gcggtcatgc gattaatacg
    49681 ggtaaagtcc tgcgcggtgc cccctggcag attgtcatct tctcgctcgg catgtatctg
    49741 gtggtttatg gcctgcgcaa tgccggatta acggaatatc tttctggcgt actcaacgtg
    49801 ctggcggata acggcctgtg ggccgcgacg ctcggcaccg gattcctcac cgccttcctc
    49861 tcttctatta tgaacaatat gccgacggta ctggttggcg cgttgtccat tgatggcagc
    49921 acggcatctg gcgttatcaa agaagcgatg gtttatgcca atgtgattgg ctgcgatttg
    49981 ggaccgaaaa ttaccccaat tggtagcctg gctacgctac tctggctgca cgtactttcg
    50041 cagaagaata tgactatcag ctggggatat tacttccgta cagggattat catgaccctg
    50101 ccggtgcttt ttgtgacgct ggctgcgctg gcgctacgtc tctctttcac tttgtaatga
    50161 gatattgata tgagcaacat taccatttat cacaacccgg cctgcggtac gtcgcgtaat
    50221 acgctggaga tgatccgcaa cagcggtaca gaaccgacca ttatctatta tctggaaaca
    50281 ccgccaacgc gcgatgagct ggtcaaactc attgccgata tggggatttc cgtacgcgcg
    50341 ctgttgcgta aaaacgtcga gccgtatgag gaactgggcc ttgcagaaga taaatttact
    50401 gacgatcggt taatcgactt tatgcttcag cacccgattc tgattaatcg cccgattgtg
    50461 gtgacgccgc tgggaactcg cctgtgccgc ccttcagaag tggtgctgga aattctgcca
    50521 gatgcgcaaa aaggcgcatt ctccaaggaa gatggcgaga aagtggttga tgaagcgggt
    50581 aagcggttga aataaagcgg cgatatccac tgcccatagt tggtaaagtg cgctttgttt
    50641 atgtcggatg cgacgctaac gcgtcttatc cgacctacaa aaccgtgcat ggcatgaaat
    50701 ctgtagcgcg tagcgcatca ggcaattttg ctatttacgc agcactcctc gcgtaagaat
    50761 tcaattatcg cttccagcat tgcgtattgc gaaacacaca tcagcgtccg actctcacgg
    50821 ttttgtttaa ccagccctac cgataccagt gctgaaatat ggtgactgag tgtagagccg
    50881 ggtatcccaa gctgcttttg caactcaccg accggcaaac cttgctcccc cgctttgacc
    50941 aggtgtttga atatgaacaa acgggtcggg tgacctagct ctttcagcgc tttagcaact
    51001 tcttccaact gcatatgaca ccccctttgt ctggttgata tttcgataat accagaaata
    51061 tcttgatctg aatttcattt cgattattct ggaaatatag atttatccaa tgaggttacg
    51121 tatgagtagt tggcttgcga tgctgcaaga tgccgcagag atgtttgtgt ttctcgccgt
    51181 cgagctttcg ttgctgttta tagtgattag tgccggtgtc agcctgataa gacaaaaggt
    51241 gccagaccat aaaatccagc agatgatggg ggccagaaaa gggagaggct atctcctggc
    51301 tgctctgttg ggagccgtta ccccgttctg tagttgctcg acaataccca tgttacgtgg
    51361 attgttgtca gcgaaagccg ggtttggtcc gactctcact tttttatttg tttcaccatt
    51421 acttaatccc attatcgtcg gactaatgtg gatgaccttt ggttggaaag tcaccttgtt
    51481 gtacgcgatt atcgccgccg gcgtctccgt tcttgccagt attatcctgg attctctggg
    51541 gtttgaacgt catatcattg ccagtaaaag ctcatcagca aattgttgtg ctctagcaaa
    51601 aacttcgccg ggaacgacat atacgcccat aaaagtgagt tgttgtagtc cagcggctaa
    51661 agccattgag aaccccgtag ttacctgttg cagtaccaaa gctgtagtaa gtattaatcc
    51721 cataaaacta gccaccaaag atgcgttgca acagtttaaa gatgtactgc catatctttt
    51781 gttaggagta ttaataggat cttttattta tggctttatt ccctcagcgt ggattgccgc
    51841 tcatgcagga gcagataatc ccttcgccat cccattaagc gccgttgtcg gtattccgct
    51901 gtatatccgg gctgaggcag ttatccctct ggcatctgtt ctgatgacaa aaggaatggg
    51961 tctgggagca ttaatggcat taatcatcgg cagtgccggc gcaagcctga cggaagtgat
    52021 attacttaaa tcaatgttca gaatgccgat gatagctgca ttcctgacgg ttatattagg
    52081 tatggctatc ttgatgggct atttgactca attgctattt taaatagatg ccagcagtaa
    52141 actataagat gcatagttag tgccatatcc ttctaagtag aaaatcatag acaggataat
    52201 ccttaaaaat cacaaatgat ttaactaatg cattgagaat gatgacggct cggtcaacaa
    52261 gtcatcaaca gacaataacc acgtaatcat gcttctctga cattttatcg gagaagcaat
    52321 gactctttgt ctcagtaccc tcctcctttt taaaaggcag atttcagtcg ctaatacaac
    52381 acatttttaa atacacttta acctggttca ttattacaca tctaaaacat cacaaaaata
    52441 ttacttcaca cactatcgga gaacataata actttttaaa atgagcgctt tttgatagtt
    52501 gttaacgttt tcctttacga tactatcact gccttttttt tatacacaaa tattatcggg
    52561 tggaaatgaa agggaattta aaggtaatta tatcaggaag atataacgca ttatatttat
    52621 tgtgtgtaga agaaaatcca ttcctgttct aattattcaa tttaaaaacc ttagcataaa
    52681 acagatgatg aataaggaat gttatgtcaa ttgactttac cccaggtata ataaatacat
    52741 atcacggcga tatttataac tgcacaacaa ataccgacaa tgttaaaaca ccagacacac
    52801 cgatgtggcc ttgtgataat tgggaagaac aacaacccat taattccata ttctctggag
    52861 aaggatacaa ccctgaacaa tttgatttag cgcaacaaca actccaacaa attaatgcat
    52921 gccacacgaa caccacatat acaaatgcag actactcaaa agttgtggtt caacttgtaa
    52981 gtcttattaa taacattgaa acgataagtt cgactccact tacgcaacag acacaatcaa
    53041 tacttaatca gattaataac attagatatg aaaaaaacaa aagtgctgaa tgtcgaatca
    53101 ttgttgtcgc taatcctaaa cccgacaagg caatcataac taaaattagc gtagaagagg
    53161 gaatacccat aacattcagt gtccaaacca tgttttcaga tactaatttt attgctgaac
    53221 aacgagctga cttaccaact aatataaaag atattcaaag cctgtatcaa aaaatgacga
    53281 agttatatat tgagcacagt gaaaataaaa acaggatgaa agtctttgcc ggaaccaact
    53341 ttattgattt caatatgaca ggacagaacc tgtccgggtt cgttctaacc ttgtcaaggt
    53401 tttatttcga agatctactt aatataaatt tcactgacgc aaatttaggt gacactattt
    53461 tttacataaa gaacacccta cccccaaatt atataaagat ggacaatatc ttgacaaaca
    53521 aatcgaaggt ttattttcaa cattattaac cattaatgac aattttttac gagcaaaagc
    53581 ggaaattgct tcgacaataa ttaaattttt agaggctagg ataaccaatc tttcatacaa
    53641 tgacatatta acatatcagc aagaattcca aaaacaatgt tataagcaag ttaaagcctt
    53701 tgcgacactc tctcgataca ataaaattca aacatgggca gaaatgtctg aatatcagtt
    53761 cgaagtattt caatacgaga cgcttaaccc caaaaaaaat gtcacacacg ccttatttaa
    53821 agagccactt cctaatgaaa aagatataaa ttatggggtg gaaatagaaa taccatctgg
    53881 caaaagaatt cgcttatcaa atcattatca gaatataata ccataatatt gattattagc
    53941 actttataat cattgaataa aaatagattt tatgtaattt taaaacaaag ggttatatta
    54001 ttgggcgatg gatactcata ccacgcccca aaataacgga attgaactaa acagaaggga
    54061 tataaacatc agacaggttt acgttactat caggcatatc acctcagaat cagatgaaaa
    54121 ctataaagaa atatctatta tggttttaat atttgttgat aaggatagta acatgaacat
    54181 gacaaaaggt gcactcatcc tcagcctttc atttttgctt gccgcatgta gttcaattcc
    54241 gcaaaatatc aaaggcaata accaacctga tattcaaaaa agttttgttg ctgttcataa
    54301 ccagccgggg ttatatgttg gtcaacaagc gcgctttggt gggaaggtta tcaacgttat
    54361 caatggcaaa acggatacgt tgttagaaat cgctgtatta ccgttggata gctatgcgaa
    54421 gcctgatatt gaagccaact atcagggccg actgctcgcc agacaaagcg gcttccttga
    54481 tccagtgaac tatcgtaatc actttgttac catcctcgga accattcagg gtgaacaacc
    54541 aggctttatc aataaagtcc cgtataactt cctggaagtg aatatgcagg gcatccaggt
    54601 gtggcatttg agagaagtgg ttaataccac ctataacctg tgggattacg gctatggtgc
    54661 attctggccg gaaccgggct ggggtgcgcc ttactacacc aatgcggtga gtcaggtaac
    54721 acctgagctg gtcaaataac accaccgaaa gatgcagatg ctttctcagc atctgcatca
    54781 tgtattacat aaaattaata tacagtaagc taactattat tattataagc cctgtcctgt
    54841 taattacctt tggcaaactg attataaagt taatgtccgc accaggagtc ggttatgttt
    54901 cttataatta ccagggatac gatgttcttc accgcgatga aaaacattct gagtaaaggt
    54961 aatgtcgttc atatacagaa cgaagaagag atcgacgtaa tgttgcatca gaatgccttc
    55021 gtcattattg atacattaat gaataatgta tttcattcta attttctcac tcaaattgaa
    55081 cgattaaaac ctgtccatgt gattattttc tcccccttta atattaaacg ctgtctgggg
    55141 aaagtaccgg tgacctttgt tccgcggact atcactatca ttgattttgt cgcactcatc
    55201 aatggcagtt actgctctgt gcctgaagcg aatgtgtcac tttcgcgcaa gcaacatcag
    55261 gttctgagct gcattgcgaa tcaaatgaca acggaagata ttctggagaa actgaaaata
    55321 tcgctaaaaa cgttctactg ccataaacac aatatcatga tgatcctcaa tcttaagcgg
    55381 atcaatgagc tggtacgcca tcagcatatt gattatctgg tgtgaatttc aggcttacgg
    55441 tgagtctggc tacgctgcca cacagattag ctaattgaaa cgcctttcac ccctgccata
    55501 ccttttaata atcgcaacag gtcttctatc gacgttgtgg catgaagctg gatatcaatg
    55561 gcgaccactt catgatcttc attttcctgc aacgaaacta aatccgtttt tattttttgc
    55621 tgcttaaacc agtcgagcat tgataccgta ttaccgttca ctaatgtcag ctgtaaatgg
    55681 taatttttgt tcatcaggcg gaaggttaat tgatggaaga cttccagcac caacaaggtc
    55741 atcactgaac cataaatacc cagttcgtac ataccgctgc caataaccat acctatggcg
    55801 gcggtcaccc agatatccgc tgccgtcgtc agacctacaa tattttggtt acgaacgaga
    55861 atgttacctg caccgataaa cccgacgccc gtcaccacct gagcagcaat acggctgggg
    55921 tcgagtccga cgtgatctaa agacagcacg tcagcaaaac catatttcga aacaatcata
    55981 aacagggcgc ttcccatgcc aattaataca tgtgtgcgta accctgctcc tttgccgcgc
    56041 atttgccttt ccatgccaat agcgccacag gcaattgccg cgagtataag acgtataata
    56101 aattctgctg tcatataaaa atgcaaaaag gagcagcaag atggctcaac ttgctactcc
    56161 tttttacttg tacctcatta attcggcaag tcattagatg cttgattttt gaaggtatac
    56221 aaatttttct gcgggttttt cttacagtat tcgatcactt taggaatttg agtgagatcg
    56281 gtttcattta aagtaacggt atcgccacct ttatatactg tttcttcatg cagcatccac
    56341 catgcaaccg gggtcattgc ttttggattc agatcaataa attcctggca ggtcatatct
    56401 ttagcggatt cattggctgc cagcgcagat tgtgcgttca ccagtgacaa agccgctaca
    56461 gcgcccataa aaataaacgc tttacggaga gatgaaatat tcattttgta acccattcaa
    56521 tatagagatt atatatgaac aggaggaata gctgctttta aagaagatat tccggcctgt
    56581 tcaatatgca aggaagtacg atgtaacgca ttcccggaat tacatatctt tcttaatttt
    56641 gtcccattcg cctttaactt tatctttaaa gttggcttgt ttatcctgag tacaagcctg
    56701 aacgatagct ggggttacgg ttgcaatacc ctgaacatct aaaaccgcat cttctggttt
    56761 atctttgttg ttcagcgctt cagcaaaacc aactgcagtt ggctggaagg attcgtccac
    56821 agccaggaaa tcttcacagg tccaggagtt gaccggtttt ttgttatcag ctgctttttg
    56881 cgcatccgct gcattgctta caactggcag aagaagcaga ccaccaagaa taacgcctaa
    56941 tacttttttc atcgtaatat cctcaactat aaagtgaaag agccgtcacg aatcaatttc
    57001 gacactgagg ttataacctg gttttctgta tatgtcatgt tgatagaaaa tatcaaaatc
    57061 agatattttt atttcaatac aatgagttac agatgcatca gatactgtaa ttaggaaatt
    57121 tttattaaat cgactgcatt cttagacgcg tttttggcat agattgatag caggggattt
    57181 tcttcttaat tttatagggt ggttctatgt tatatataga taaggcaaca attttgaagt
    57241 ttgatctgga gatgcttaaa aaacatcgca gagcaatcca gtttattgcc gtgctgttgt
    57301 ttatcgtcgg gttgctgtgt atcagtttcc cgttcgtctc tggcgatatt ttaagcacag
    57361 tagtgggtgc attattaatc tgctcgggca ttgcgcttat tgtcgggtta ttcagcaacc
    57421 gcagtcataa tttctggccg gtattatccg gtttcctcgt cgcagtcgcc tatttattga
    57481 tcggctattt cttcatccgc gcaccggagc tgggcatttt tgccattgcg gcatttattg
    57541 ccggtttgtt ctgtgttgca ggggttattc gcctgatgag ttggtatcgt cagcgttcaa
    57601 tgaaaggcag ctggctacag ctcgttattg gtgtgctgga tatcgtcatt gcctggatat
    57661 tccttggtgc aacgccgatg gtgtctgtaa cgctggtttc cacactggtg ggaattgaac
    57721 tgatatttag cgccgccagc ctgttcagct tcgccagttt gttcgttaag cagcaataat
    57781 tatcccggat gtcacccgga tcatagtcac ttgatgtgac tatgatccga ttaatactct
    57841 caccgctacg cagcgttgta gatcaattgc gcactatcat tgaaataatt acctgctagt
    57901 gattatttca acctactgaa tttcatctaa tttttttcac tccatggcaa attagccatt
    57961 tcaaacatta tcatggctga tattttccgt agtcaggttt aatgttttaa aagtgcagtg
    58021 ggaaagtgaa caaagagttc cgtaagcgtt gatgctatgg gcggttaaat aagtaatccg
    58081 ggttcatttt tttgcaactg gcattgatta cattgcataa atatccgtgt ctccagacgc
    58141 tatataaaaa cctgaagaca tgaatgcgtt atttactcag gtaatttcaa tgcgttaaaa
    58201 gaaagctggc aatccaattg ccagcttaag tcgaaacaag gagactcgat atttaaatcg
    58261 gattacattt taactttagt aatattcttc agagatcaca aactggttat tgataactta
    58321 ttcttgggca gtaatccgca aacgttaact ttttgtttac tgtttacaaa ctgataacaa
    58381 ccaggaatct tacttaggat caatatatgg agtgcgtgat ggataaatct gaagtattga
    58441 ttagtgttaa tagacgtatt agttcacgaa gggcaaagtt cttataggcg tttactatat
    58501 tgaacaacga ttcggacaag gatgtaaata atgaaaagga tgacatgttc gaaacgataa
    58561 cggctaagga gcaagttatg atttttctca tgacgaaaga ttcttttctt ttacagggct
    58621 tttggcagtt gaaagataat cacgaaatga taaaaatcaa ttccctgtca gagatcaaaa
    58681 aagtaggcaa taaacccttc aaggttatca ttgataccta tcacaatcat atccttgatg
    58741 aagaagcgat taaatttctg gagaaattag atgccgagag aattattgtt ttggcacctt
    58801 atcacatcag taaactaaaa gctaaagcgc ctatttattt tgttagccgc aaagaaagta
    58861 tcaaaaatct tcttgagatt acttatggta aacacttgcc ccataagaat tcacaattat
    58921 gtttttcaca taatcagttc aaaattatgc aactgattct gaaaaataaa aatgaaagca
    58981 atatcacgtc gacgctcaat atttcgcaac aaacattaaa gattcagaaa ttcaacatta
    59041 tgtacaagct gaaactaaga cgtatgagcg acatcgtcac cctgggtatc acatcttatt
    59101 tttagtcagg acataagcaa ctgaaattga tggctggcat gacgagggat gcagatgctg
    59161 atttcattac ccccggtgat tactaaagga gaggctaaaa cgactttatt cccctggtat
    59221 gtgtatccac cagtagaacc cttcgttgcc cgaatgctgg caggaactgt tggcagaacg
    59281 gcaacatttt ttttgtcgtt gacctcacca tgtcgatcac tatacctgta tcccacctta
    59341 ctggctgaca accccactat gccgctggtc tgtaaatccc tcatatctct cctcgcgcgc
    59401 aatttaaaga accgttattt ctcaagaatt ttcagggact aaaatgaaca gaagaagaaa
    59461 gctgttaata ccgttgttat tctgcggcgc gatgctcacc gcctgcgatg acaaatcggc
    59521 ggaaaacgcc gccgccatga cgcctgaggt cggtgtcgtc acactctccc ccggttcggt
    59581 caatgtgttg agcgaattgc ccggtagaac cgttccttat gaagttgccg agatacgtcc
    59641 ccaggtgggc ggtattatca ttaaacgcaa ctttatcgaa ggcgataaag tgaaccaggg
    59701 cgattcgctg tatcagattg atcctgcacc tttacaggcc gagctaaact ccgccaaagg
    59761 ctcgctggcg aaagcgctct ctaccgccag caatgcccgc atcaccttta accgccaggc
    59821 atcgttgctg aagaccaact acgttagccg tcaggattac gacaccgcgc gcacccagtt
    59881 gaatgaagca gaagccaatg tcaccgtcgc caaagcggct gttgaacagg cgacgatcaa
    59941 tctgcaatac gcgaatgtca cctcgccgat tacgggcgtc agcgggaaat cgtcggtgac
    60001 cgtcggcgca ctcgttaccg ctaatcaggc agattcgctg gttaccgtac aacgtctgga
    60061 cccgatttat gtcgatctca cgcagtcggt gcaagatttc ttacgcatga aagaagaggt
    60121 cgccagtggg caaatcaaac aggttcaggg cagtacgcca gtacagctca atctggaaaa
    60181 tggtaaacgc tacagccaga ccggcacgct gaaattctcc gacccgacgg tggatgaaac
    60241 cacgggctcc gtgacgttac gggcgatttt ccccaaccca aatggtgact tgctgcctgg
    60301 catgtacgtc acggcattag tggatgaagg tagccgccag aatgtattac tggtgccgca
    60361 ggaaggcgtc acccacaacg cccagggtaa agcaacggcg ctcattctgg ataaagacga
    60421 tgttgtgcag ctacgcgaaa ttgaagccag caaagccatc ggcgaccagt gggttgtcac
    60481 ctctggcttg caggctggcg atcgggtgat cgtttccggt ttgcaacgca ttcgtccggg
    60541 tatcaaagca cgagcaattt cctccagcca ggaaaacgcc agcaccgaat cgaaacaata
    60601 acgttgcagg cttaagggga ctttcatggc taactatttt attgatcgcc cggtttttgc
    60661 ctgggtactt gccattatta tgatgcttgc aggtggtctg gcgatcatga acttaccggt
    60721 tgcgcagtat ccgcagattg cgccaccgac cattaccgtc agcgctacct atccaggtgc
    60781 cgatgcgcaa acggtagaag actcggtcac tcaggtgatt gagcaaaata tgaatgggct
    60841 tgatggcctg atgtacatgt cttcaaccag tgatgcggcg ggtaatgcct ctatcactct
    60901 gaccttcgag actgggacat ctcctgatat cgcacaggtt caggtgcaaa ataaactgca
    60961 actcgctatg ccttcattac ctgaagcggt gcagcagcag gggattagcg ttgataagtc
    61021 gagcagtaat attctgatgg tagcggcgtt tatttctgat aacggcagcc tcaaccagta
    61081 cgatatcgcg gactatgtag cgtctaatat caaagacccg ctaagccgta ccgcgggcgt
    61141 tggtagcgta cagctctttg gttccgagta cgccatgcgt atctggctgg acccgcaaaa
    61201 actcaataaa tacaatctgg taccttccga tgttatttcc cagattaagg tgcaaaacaa
    61261 ccagatttcc ggtggtcaac tgggtggcat gccacaggcg gcagaccagc aactaaacgc
    61321 ctcgatcatt gtgcagacgc gtctgcaaac gccggaagaa tttggcaaaa tcctgttgaa
    61381 agttcagcaa gatggttcgc aagtgctgct gcgtgatgtc gcccgcgtcg aacttggagc
    61441 ggaagattat tccaccgtgg cgcgctataa cggcaaacct gctgccggga tcgccatcaa
    61501 actggctacc ggagcaaacg ccctggatac ctcgcgggca gtcaaagagg agctgaaccg
    61561 cttatcagcc tatttccccg caagtctgaa gacggtttat ccttacgaca ccacgccgtt
    61621 tatcgaaatt tctattcagg aagtttttaa aacactggtt gaggctatca tcctcgtctt
    61681 cctggtcatg tatctgtttt tgcagaattt ccgtgccaca atcatcccga cgattgccgt
    61741 accggtggtt attctcggga cgtttgcgat cttgtcggcg gtcggtttca ccatcaacac
    61801 gttaactatg ttcgggatgg tgctggcgat agggctactg gtggatgacg ccatcgtggt
    61861 ggtggagaac gtcgagcgtg tcattgcgga agataagcta ccaccgaagg aagcgacgca
    61921 taaatcgatg gggcagatcc aacgtgcgct ggtcggtatt gctgttgttc tttccgcagt
    61981 gtttatgccg atggccttta tgagcggcgc aaccggggag atctaccgcc agttctccat
    62041 cacgctgatc tcctccatgc tgctttcagt atttgtggca atgagcctga cccctgccct
    62101 gtgcgccacc attctgaaag ccgcgccgga aggcggtcac aaacctaacg ccctgttcgc
    62161 acgcttcaac acgctgtttg aaaaatcaac tcagcactat accgatagca cccgctcgct
    62221 gttgcgttgt accggtcgct acatggtggt ctacctgctg atttgcgccg ggatggcggt
    62281 gctgttcctg cgtacaccga cctctttctt accagaagag gatcaggggg tatttatgac
    62341 caccgcgcag ttaccttccg gtgccaccat ggttaacacc acgaaagtgc tgcaacaggt
    62401 gacggattat tatcttacaa aagagaaaga taatgttcag tcggtgttta ccgttggcgg
    62461 ctttggcttc agcggccagg ggcaaaacaa cggcctggcg tttatcagtc tcaagccgtg
    62521 gtctgaacgt gtcggtgagg aaaactcggt taccgcgatc attcagcggg caatgattgc
    62581 gttaagcagt atcaataaag ccgtcgtctt cccgttcaac ttacccgcgg tggctgaact
    62641 gggtaccgcg tcaggttttg atatggaact gctggacaac ggtaacctgg ggcacgaaaa
    62701 actaacccag gcacgaaacg agctgttatc actggcagcg caatcgcctg accaggtcac
    62761 cggggtacgc ccgaacggcc tggaagatac gccgatgttc aaagtgaacg tcaacgccgc
    62821 gaaagctgag gcaatgggtg tggcgctgtc tgatatcaac cagacaattt ccaccgcctt
    62881 cggcagcagc tacgtgaacg acttcctcaa ccaggggcgg gtgaaaaaag tgtatgtcca
    62941 ggcaggcacg ccgttccgta tgttgccgga taacatcaac caatggtatg tacgcaacgc
    63001 ctctggcacg atggcaccgc tttctgccta ctcgtctacc gaatggacct atggttcacc
    63061 gcgattggaa cgctacaacg gcatcccgtc aatggagatt ttaggtgaag cggcggccgg
    63121 gaaaagtacc ggtgacgcta tgaaatttat ggcagacctg gtcgctaaac ttccggcagg
    63181 cgtcggctac tcatggaccg gactgtcgta tcaggaagcg ttatcctcaa accaggctcc
    63241 tgcactgtat gccatttcac tggtcgtggt gttcctcgcc ctcgccgcac tctatgagag
    63301 ctggtcaatt ccgttctcgg tgatgttggt tgttccgtta ggcgtcgttg gcgcattact
    63361 ggccaccgat ctgcgcggct taagtaatga cgtctacttc caggttggtt tgctgaccac
    63421 catcgggctt tccgccaaga acgccatcct gattgtcgaa tttgccgttg agatgatgca
    63481 gaaagaaggg aaaacgccga tagaggcaat catcgaagcg gcgcggatgc gtttacgccc
    63541 aatcctgatg acctctctgg cctttattct cggcgtgctg ccgctggtta tcagtcatgg
    63601 tgccggttct ggcgcgcaaa acgcggtagg taccggcgtg atgggcggga tgtttgccgc
    63661 aacagtgctg gcaatttact tcgttccggt ctttttcgtt gtagtggaac atctctttgc
    63721 ccgctttaaa aaagcgtaac gtgtaaatga gagtaaggtt gaacgtgaag gttcagcctt
    63781 actctttcct gctaaccatt caccactcaa caaccagcta accgtcagga ataccaggct
    63841 tagtacacag cactgaaagt agaaaccact ccagccattc gtcatcaatt tgaacagcac
    63901 aatactgacc cacattcccg taatcgtatg aattgtcagc ttaataatcc caaacatgcc
    63961 gagtcttttc ctcctgaaaa tacctatacc gcctggcaaa ctgcgaaaga gaactagccg
    64021 gtagcccggc agaaatcatc agggaagagt ttcacatgaa gcaggtgtga gatcctgacc
    64081 aatattcaaa tgcaaaatat gtcaggaaaa ggtacctggc gaatgttgcg caaactgatg
    64141 tggcgttaca ccataatatt gtcgaaatgt gtttataaag tacgacgtac tgctatagcc
    64201 acatttttct gcaatagtat gcagaggaat ttgacgtaac tcgagtaatc gtctggccat
    64261 cgacatcctg gaagcgagta atattttact gaaacaggta ttttcatcct gcaacttttt
    64321 tttgatgaga ctctcgctgg tatacattct ttccgcgata tcgcgcagat accaacgttt
    64381 ggcgatatca aagctaataa ggcgttcaac ttttcctgaa acagtgctga tgctattgaa
    64441 aagtaagggg atcagttctt tcttatgaga aaacatcgat aaacaggaaa ggtagagcat
    64501 attgctcagt atcggtgaat ttaccccgga ttcattgaag ctatcaaaaa tcgccgtcac
    64561 cagcggcatg gggggtgact ggttaattaa tagacggtct ttgctatgta accctaaatt
    64621 gcgtggtagc tgacgaatat tttgtaagta atggcaaact gtcagctcat cgatatccag
    64681 tcgtcgcgta tccggtgaac aaaacgcaaa atcgtctgcc aaatttttct ccagcagcac
    64741 gatactctcg ttatgcagcg atattttatg ctgttcatga taaatatcga atgaacgacg
    64801 aatgaggatc accgagcaga catgagtcat gattatccct tatatttcat actgcgatta
    64861 tttcaatttc agtatactaa tgaaatgttg ccagactgtt tcttagcgca aaaactgcca
    64921 gatttggtag gaccaaaatt tatccacaaa gtagttttgc ataacagtta attgataata
    64981 agtaaatgca gcacgaatat attttcgcac agcgtatagc ttatgtttat aaaaaaatgg
    65041 ctgatcttat ttccagtaaa agttatattt aacttactga gagcacaaag tttcccgtgc
    65101 caacagggag tgttataacg gtttattagt ctggagacgg cagactatcc tcttcccggt
    65161 cccctatgcc gggttttttt tatgtctgag taaaattcta taatcttatt ccttccgcag
    65221 aacggtcagt gccgtaaaaa tttccttgcg caacaatact tgccgccgag tcacggttcg
    65281 gcaatccctg cgccgatcgc tcctgatact ctgtgggcgt catcccataa taatttcgaa
    65341 agacgtaaat gaaatacgac acgctgtgat atccacagga cactgcgact cgcttaattg
    65401 aaaagccatg tataacaata agttgcaaag cacgttgcat tctacactca gtaagcaact
    65461 gtgaatatga tgtctcttct tcgcgcaatt ttttctttaa caggcttgga ctcatcaaca
    65521 gctcgctggc gattcgagct agtgtccact catgggcgat attattattg ataaccgtac
    65581 aaactcgtgt tcgcatgttc ggttgcaaaa cgttcaaaag cagcggtata aagtgctcat
    65641 cctcaagaaa aacagataac aacgcaaaga ttaatgctct tttacgtagc atttctgcat
    65701 agcgaagatt cttattctgc gatagttgcg caacttcctg aaaaacagga atatctctgc
    65761 tacagtgatg aacgagtaat gaagaaattt ttttatgtgc atgaagattt aatgcctcct
    65821 ccttgagcat cggcagaaat aatgaaagcg tatcccttga tactaataca aaattttcaa
    65881 cacacttatc tactcgaatt tggcttgcat ccgcaaaaac caggtcaccg ccattaaaat
    65941 agcgatattc accattaacc atggtgagaa tatatttatg tcttgcgtac gcaattagac
    66001 aattcccatg taatgattgc atagttgact taatattaca taaacatatt actgttaatt
    66061 aatgtagcac gccgccctct taagtcaaat tgattgataa ataaatttaa acgaagcggg
    66121 agaacaggat gataatgtgt gatgttctac gggcaggatg actggattta taatacaaaa
    66181 acgtgtttaa gaacgcagtg aaattaatag caagcaaata atcccttttc gtgacattaa
    66241 aggcaatcgc tacatttaat aaacattcat ataacatata tcttatcaac acgatgaata
    66301 gacagccaat atattattgc gattaataag caaccgaatg cccagctgtt tttttaaagg
    66361 ctgggcattc ggtttttaca acgttatgtt atcaggtgtg tttaaagctg ttctgctggg
    66421 caataccctg cagtttcggg tgatcgctga gatatttcag ggaggctttg tagtcttcca
    66481 gcaacagttc agcaaagtcc atttcgaagc cgcgacgaca cataatgcgc atcaccacga
    66541 tgtcggtggc ttcaccgccg agagtgaagg ccggaacctg ccagccgcgc agacgcagac
    66601 gttcagagag gtcgtacagg gtgtatcccg gatcttcacc atctttcagt ttgaagcaaa
    66661 ccgccgggat gccttcgtcc gggcgacccg tacagatgaa ctcatacggc cccagtttgg
    66721 cgatttcatc cgccagataa gcggcaacct ggtaagaggc gttctgtact ttggtatagc
    66781 cttcacgacc gaggcgcagg aattcatagt actgtgcaat tacctgaccc gccgggcggg
    66841 agaagttgat ggcaaaagta ccaatttgac cacccaggta gtcaacgttg aacaccagtt
    66901 cctgcggcag cgcttcttcg tcacgccaga taacccagcc gcagcccagc ggagccagac
    66961 cgaatttatg gcctgaagca ctgatcgatt tcacacgcgg caggcggaag tcccagacga
    67021 tatccggggc gacgaacggt gccaggaagc caccgctggc agcgtcgatg tgcatgtcga
    67081 tgtcgatacc agtgtcggcc tggaatttat ccagcgcatc gtgcagcggt tgtgggaact
    67141 catagttacc ggtgtaggtc acgccgaaag tcggcaccac gccgatggtg ttttcgtcac
    67201 aggcttcaat catgcgtttc gggtccataa acaactgacc ggggcgcata gggatctcac
    67261 gcagctccac atcccagtag cgggcgaatt tatgccagca gatttgtacc ggaccgcaca
    67321 ccaggtttgg tttatccgtt ggtttgcctg cagcttccat acgcttgcgc caacgccatt
    67381 tcatcgccat cccgccaagc atacaggcct cggaagaacc aatggtgttg gtgccaacgg
    67441 cctgaccatt tttcggcgca ggcgcatgcc acagatcggc aaccatattt acgcaacgca
    67501 ggtcgatggc tgcggattgc ggatattctt ctttgtcgat ccagttttta ttgatcgaca
    67561 aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg gccaggttct
    67621 gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg acatcatcgc
    67681 gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc tttgcgccaa
    67741 aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc atttcgaact
    67801 ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga agcaaggcat
    67861 tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta taatataaac
    67921 atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg cccaaacatt
    67981 cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg tacaccccgt
    68041 tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc tctttgccta
    68101 cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca ccgcgatgga
    68161 agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc aatttatcac
    68221 gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc ccaaagttaa
    68281 agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata ccaccatgac
    68341 aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc gccgtcagcg
    68401 cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt aatgttttct
    68461 caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga tagacgccga
    68521 ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg tcccaggatt
    68581 tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc aatgttgccg
    68641 cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc ggcgcgttaa
    68701 tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg ccccccgcat
    68761 tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg ggatcgtgat
    68821 acagcgcaaa gcccaacgcc actttttgcg catcggtagg caatttttgc gggatgggct
    68881 gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat tccgcgcgct
    68941 gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta cccgcccagt
    69001 gtagcgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat tcaatcttat
    69061 tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg gcctcgaggt
    69121 taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg ccaggaatat
    69181 aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct ttttcgcgga
    69241 gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg gcctgtttac
    69301 tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa atcgcgacgc
    69361 cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc tcacggtggt
    69421 gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg agacaaagcc
    69481 atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca tgaatataaa
    69541 ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc atgattgcgg
    69601 cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta tttttataaa
    69661 ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat ccaccgcacg
    69721 ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg ataattagcg
    69781 acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc taatccagac
    69841 gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt aaaactggat
    69901 gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc ctctgatgcg
    69961 ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc gaaaattttc
    70021 cccgacagta aaacctttcc cgactgcgca cccaaaatgg acccgctgga tattttaatc
    70081 cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt tgaaaatcac
    70141 ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa ttccctgaaa
    70201 gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca cattccgtgg
    70261 tcttctctgc tggcgctacc gcagtcatat attgtcccgg gcggccgttt tagcgaaacc
    70321 tactattggg attcctattt caccatgctg gggctggcgg aaagtggtcg ggaagatttg
    70381 ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca catccccaac
    70441 ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct gatggtggag
    70501 ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct taaaatggaa
    70561 tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta tcgccatgtt
    70621 gtgcggatgc cggacggatc gctgctcaac cgttattggg acgatcgcga cacgccgcgt
    70681 gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc gcccaacgag
    70741 gtgtaccgcg atttacgcgc gggagcggcc tcaggttggg attactcttc ccgttggctg
    70801 cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat cgatctgaat
    70861 gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa aggcgagaaa
    70921 gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt aaaccgttac
    70981 ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga acaactggcg
    71041 ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca tgaacaggcc
    71101 gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat tctggcaagc
    71161 gagtacgaaa ccggtgaaca gtgggataaa cccaatggct gggcaccgtt acaatggatg
    71221 gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat cgcgcgcagc
    71281 tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga gaaataccat
    71341 attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca ggatgggttt
    71401 ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc ataatatttt
    71461 tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga ggagatattt
    71521 aacatcattg ccgcctgggt gcgatttttc acttccagac ggcgatacag ggattccaga
    71581 tgcgctttta ccgttccggt actgatattc agcgctctgc cgatctcctt atttgattcg
    71641 cccgccgcta acatggttaa aatctcccgc tggcgggcgc ttaacgattt gagatcttta
    71701 atgtcctttt ccggcgtcgt ccgccagtct ccaggcagaa acatcatccc catcgccgca
    71761 ctatttaccg ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat ggccagcaca
    71821 ttaaaatgaa taacttcctg taaccaccgt ttattgcaat ccgtcgccgt gattaacacc
    71881 ttaacctcag gaaactgcac cacggttttt tgcagcaacc agtagcaaaa ctcaccatcc
    71941 tgatcgccat cgagcataac taaggcttca gggtaacttt ccagcttttg ccataactcg
    72001 tctgcctgac tggccccctg aatactcact cctggaatac gctgctgtaa actgattttc
    72061 attccatgaa taaatattga ctgcctgtca aacatgacta tttgcataac tgaatctcca
    72121 cctgaatacg ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga aaaaataaga
    72181 attcgccatt tggcggtggc cattctacag agatgacgcg tagaaaatag ttaccgatat
    72241 aaatagttac agctaaacgc ctgaaattac atgtcgaggg cactatttaa aacaattttg
    72301 aggatttcct tatattggtg gttagtacgc atgcaattaa aaatgaaact ccgcgaccac
    72361 aagcaaaaat aacaaacggc aatgagacaa aaataagcac aaatagccaa cacgtcctct
    72421 attcacttta aagggaatcg ctgaaaaata cgctctgttt agggggattc acctttctca
    72481 gaaagctatt ccgccctttt cctgctgaga aatcgccaca ttcgtcatga caacattgtg
    72541 aaacccggca ttagatgtta gaaaaaacaa taaacaatgc gatatgcgcg ttactgtttc
    72601 gctgtgaaca acaatcggtc aaagaaatgg ataaaattca cgcaatgcag ttgttcatca
    72661 aagtcgcgga gctggaaagt ttttcccgcg cggcggattt ctttgctttg ccaaaaggaa
    72721 gtgtttcgcg ccagatacag gcactggaac atcaacttgg cacccagctt ctccagcgca
    72781 ccacgcgacg ggtcaaactc acgccagaag gcatgaccta ttatcaacga gcaaaagatg
    72841 tgttgagtaa tctcagcgaa ctggacggtc tgtttcaaca ggatgccacc agtatcagcg
    72901 gtaaattacg catcgacatc ccgccaggaa tcgcgaaaag cctgttactg ccgcgcctgt
    72961 cggaatttct ctatctgcat ccgggaattg agctggaact gagtagccat gaccgtccgg
    73021 tagatattct tcatgatggt tttgattgcg tgatacgcac tggcgcgtta ccggaagatg
    73081 gcgttatcgc ccgtcccctc ggcaaactga ccatggtcaa ctgtgccagt ccgcactatc
    73141 tgacgcgctt tggttatccg caaagccccg atgatctgac ttcacacgca atagtgcgtt
    73201 atacaccgca cctgggtgta catccgttag gttttgaggt tgccagcgtt aatggcgtcc
    73261 agtggtttaa gtctggcggc atgttgacgg taaacagtag cgaaaactat ctcgccgccg
    73321 gtcttgccgg tctggggatt attcagatcc cgcgcattgc cgtgcgcgaa gccctgcgtg
    73381 ccgggcggct tattgaagtg ttgccaggct accgtgccga gccgctctcc ctttcgctgg
    73441 tttacccgca gcgtcgggag ctttcccggc gtgtaaacct gtttatgcag tggctggctg
    73501 gcgtaatgaa agagcacctg gactgaccga ctatactttt taagaatgac cacgacagaa
    73561 ggacaaaaga gcggatgacg caggaaaacg agatcaaacg tcccacccag gatctggagc
    73621 acgagccgat taagcagttg gataatagcg agaaaggcgg caaagttagc caggcgctgg
    73681 aaaccgtcac caccactgcc gaaaaagtcc aacgccaacc ggttattgcg cacctgattc
    73741 gtgcgacaga acgctttaat gatcggctgg gtaaccagtt tggtgcggct atcacctatt
    73801 tctcattttt gtcgatgatc ccgattttga tggtgtcgtt tgccgcaggg ggctttgtgc
    73861 tggcctccca tcccatgttg ctacaggata tcttcgacaa aattctgcaa aacatcagcg
    73921 atccgacgct agccgccacg ttgaaaaaca ccatcaacac cgccgttcag cagcgtacga
    73981 ctgtagggct tgtcggcctg gcggtggcgc tttattccgg catcaactgg atgggtaacc
    74041 tgcgtgaagc gattcgcgcc cagtcgcgcg atgtctggga acgctcgccg caagatcagg
    74101 agaagttctg ggtaaaatat ctgcgtgatt ttatttcgct gattggtttg ttgattgcgc
    74161 tgattgtgac gctttcgatc acctcggttg ccggttcggc gcagcaaatg attattagcg
    74221 ccctgcacct gaacagcatt gagtggctga aaccgacgtg gcggttgatt ggcctggcga
    74281 tttccatctt cgccaactat ctgcttttct tctggatctt ctggcggttg ccgcgccacc
    74341 gtccgcgcaa aaaagccctg attcgcggaa catttctggc tgctattggt tttgaagtga
    74401 ttaaaatcgt gatgacctac accttgccat cgctgatgaa atccccctct ggcgcagcat
    74461 ttggttccgt gctggggctg atggcatttt tctacttctt cgcccgtttg acgctgtttt
    74521 gcgcggcgtg gattgccacc gccgaatata aagacgaccc gcgaatgcca gggaaaacgc
    74581 agccttaaaa taggccggat gcggcgtaaa cgccttatcc ggcatacgat tttattcggc
    74641 ctggctcccc gtaggccgga taagatgcgc cagcatcgca tccggctata atgcgcacat
    74701 aacctcttga aactcttccc cagagcctct tcagccatct atttgggagc aaacaatttc
    74761 attccaactc ataaccccag catataaatc cagttggtaa cttttattta acctgaaacc
    74821 agttttatcc actatttata aaattatgtg aagcatttca tagaagaaaa atcactggcc
    74881 taaacattat cccctttttg cctggttttt gaccatttcc gcgatttgtt acacattgaa
    74941 atatcacttt tgctgtgcgt aatatggcta ttcgttagcc aaaaaataag aaaagattat
    75001 gcaagcaaca gccacaacac tcgaccacga gcaagaatac acgccgatca actcgcgtaa
    75061 taaagtcctt gtcgcctctc tcattggcac ggccattgag ttcttcgact tttacattta
    75121 cgccactgcg gccgttattg tgtttccgca tatcttcttc ccgcagggcg atccaacggc
    75181 agcaacgcta cagtcgctcg ccaccttcgc catcgccttc gtcgcgcgcc ccattggctc
    75241 tgccgttttt ggtcattttg gcgatcgcgt tgggcgtaaa gcgacgctgg tcgcctcgtt
    75301 gctaacgatg gggatttcga ccgtggtgat tggtctgctg ccgggctatg ccacgattgg
    75361 tattttcgcc ccactgctgc tggcgctggc tcgatttggt cagggtctgg gcttaggcgg
    75421 tgaatggggc ggcgcggcgc tgctggcgac tgaaaacgcc ccaccgcgca aacgtgcact
    75481 gtatggctcc ttcccacagc tgggcgcacc gattggcttc ttctttgcca acggcacttt
    75541 cttgctgctt tcctggctac tgaccgatga gcagtttatg agctggggct ggcgtgtgcc
    75601 atttatcttc tcggcggtgc tggtcattat cggcctgtat gttcgcgtgt cgctgcatga
    75661 gtcgccggtg tttgagaaag tcgctaaagc gaaaaaacag gtgaagatcc cgctgggtac
    75721 gctgctgacc aaacatgttc gcgtaaccgt actgggtacg ttcatcatgc tggcaacata
    75781 tacgctgttt tacatcatga cggtctactc tatgaccttc agtaccgccg ccgcgccagt
    75841 tgggcttggc ctgccacgta acgaagtgtt gtggatgctg atgatggcag ttattggttt
    75901 tggcgtgatg gtgccagtcg ccggattact ggctgatgcc tttggtcgcc gtaaaagcat
    75961 ggtaattatc accacgctga tcatcctgtt cgcgctgttc gcctttaacc cgctgctcgg
    76021 ttctggcaac ccgattctgg tttttgcctt cctgctgctg gggttaagtc tgatggggct
    76081 gaccttcggg ccgatgggcg cgctgttacc agagctgttc ccaacagaag tgcgttacac
    76141 cggagcatcg ttctcttaca acgtagcgtc gattctcggg gcttccgttg cgccgtatat
    76201 cgcagcctgg ttgcagacta actacgggtt aggtgcggtg gggttatatc tggcggcgat
    76261 ggctggcttg acgttaatcg ccctgctgct gacccatgag acgcgacatc agtcgttgta
    76321 agtatcgtgt gggcagccac cctcacccca gccctctccc tgaaagggag agggagaaaa
    76381 gcggagcgag atgttatatc gcgaacataa cagccctccc ccagaaagtg aacggaaaag
    76441 cggtactcgt tgtttcaaca cgcacaaaac agtcccctcg cccctttggg gagagggtta
    76501 gggtgagggg aacacgctgc tcccgaactc accagcaaac acaatccaac ctcatcactt
    76561 cttcatctgc gacaatatcg tccgacactg attagcctcc ccttcggaag gtgagatcag
    76621 tgccagtaac gccgccgccg gtgttaccag cgttgccagt gccgcagcaa cagcaccacg
    76681 ggcaatcaac ggcccggctt tcaccccagc ctgcggattt ttaaacgtcc cacgcacata
    76741 cagcggcgaa cgcagtgtga taatccgaat ccctttactc tccggatcaa tagtcaaatc
    76801 cagctgttcc gaagcaaaac ttgccgtgcc ggtaacatta atcaacgcgt tctcagtatc
    76861 gaaagcaaaa atctgcgggc gcgccacgcc gttggcaata ttcagattcg ccgccgcgca
    76921 gttcacccgc acctcatcgt caccaaatat cgcaccgaca atgtagttgc cgacattcag
    76981 cccaacaatc tccatcaggt tgcggctcac cagcccgtca ttcatcaaca gtttcaggtt
    77041 gccgttactg ttgcctaaaa gcgccgccac cgagttaccg ctaccgcgta gttccgcgtc
    77101 accgttcatt tcccccagcg tcttctgcat cagttccaca tcgggcatca gttctttcag
    77161 tttcagtcga cgagcctgaa tatctgcccg cccctgcatc ggctttttat cgccttccag
    77221 atggatattc gccgcaatgc tgccgcccgc catgccaaat ttcagcggtt gcaggcgcag
    77281 gtcagcattt ttgaggatga tatgagtaga aagatcgcta atcggcaggc tactgccatg
    77341 ctcaatgcgc cgccctttga agcgaacatc ggcatccata acgtcccatt tatcggtttc
    77401 gaagcggtca taaggcagca ctttgcccgc aggctgaacg cttttttcgc ccttcttctg
    77461 ttcagaccgt ttcgactttt ctgccccttt cccggagtca acgccaatta acggtcccag
    77521 gtccgccagc cgcaattgcc gcgactcgac atcaccttcc agttttggtc gtggctttcc
    77581 ggtggtgtag accagagaac cgtggatatc gctatcgcca attcgcccat taaaaccgcg
    77641 ataatcaaag accgacgatt tttcagtgtc gattttcgct accagccgac catccgtttc
    77701 aaacggcggg gtatcgggca gcagaacgcc cgtcagttca tagagatcac ccagtgaatc
    77761 gccagaaaat ttaagccgta aatcgacacc gcccatcttc attgggtcat tcacgacgcc
    77821 atcaaaagca acacgggtgt taccagagcg gaaatcagcc tgtaccggaa acggcgtccc
    77881 ttcgccacgc agcgccagca taccgcctat ttttcccgta cccgtgagcg gttcaccgtt
    77941 ataacgtccc tgcgccttca ggccaaaaac gtaatcgccc accttttctt tatccgcttt
    78001 acctttcgat ccagtaactt cgctgaacgg cagcggcttg cctaacggat caacaaaaat
    78061 ctccagatcc gctttgctta ctttgtcatc aatagcgatc cgcccttgat cgaaaagaat
    78121 attatccagc cgaaacgacc atgccgacgg ctttgcattc gcgtctttgt tatcatcgtt
    78181 ggcgagatta aacgtccagt tattgttctt ttcggagagg cgaatcaggc gcgcgtcggg
    78241 cttttcgagc ttgatccacg gcagccagac cgttttggtc agcagcgcca gcggggccag
    78301 cgttgcctct acgcgtggca aatgcaccat cgtgacttcg ggaatatccg gtggattgcc
    78361 aagaatgatg tcttccgcgt gtacatgggg ccacggcacc cagctgcgcc agccagtttc
    78421 ttgtttttgc cgctcccaca ccacgcccag atcgccacgg atagcgaacg gacgattcaa
    78481 ctccgcagag actttctggt tgatggtcgg tttgagtcga ttccagtcaa aagttgcaat
    78541 caaaatgatc gccacgacaa tcaacaacaa gaaagccact gaaatcgcag cggttatttt
    78601 gcctgccttg ctcatcgttc gcttcctcat attcttcctg tgccagtcct aaagatagtc
    78661 cagccaggcg gaaaatgagg cagcttatag cgccagaacc gccgtattca gcgtttctat
    78721 cggtgccggg cgtgaaagaa accagccttg tgcggcgaat gcgggcgagt tctgaacatc
    78781 acgccactct tccggcgttt ctacgccctc aacaatcacc ccgcgacaat agcgattcat
    78841 tagatgtaaa agctgagaaa agagtgtgcg tccttccggc gactgacgca gcatcacaaa
    78901 cagttctcgc gcgattttga tgtagtcata acgcacttca cttagtgcag agaaatttgc
    78961 catcccggta ccaaaatcat ccagccacag cgggccaaat tcacacatcg aggcaaaggt
    79021 tgaatctttc ggcagacgga tatgctccac cagttcgaaa cgcagccagg gaagacgctc
    79081 aatctggcgc aggatttttg gttgctgacg cagggcgatg agcgtagggc catcaatatt
    79141 gaccgatgcc agcaggccgt gctctataaa gaagtcggct ttttgcgcca gcaaatcaat
    79201 ctgctctttc acaacctcca tccgatggct gacggtgatt tcagtaaaat agcgatccgg
    79261 cggcaggcgt tgcgaagggt tcaagggatg cgcgaccacc gttaatagct ccacggccat
    79321 taaccgcccg catgtttgat agatcggctg ccaggtgtaa gcacgctcac actgcaacca
    79381 aaaacgccgt tcctgcaagc tctcgatgct tgcttcaggg ttgcttattc gctggataac
    79441 ctgccttatc atcaaagatg tcctgtgttt aaaagtgatg ctgcccggac tcgacaaaga
    79501 ttatcggcgc gtaagggcag aactttaccg cctggccggg gtaaatttcc tcactccaca
    79561 acggaatctt tcaggaatac gtcctggctc aaaaaaataa tggaacactg ttttaatatg
    79621 gttgaccagc aaaccaccac agcgcaaact aacgctaatt ttttacagat caggttcacg
    79681 actatgtcca aaaagattgc cgtgattggc gaatgcatga ttgagctttc cgagaaaggc
    79741 gcggacgtta agcgcggttt cggcggcgat accctgaaca cttccgtcta tatcgcccgt
    79801 caggtcgatc ctgcggcatt aaccgtccat tacgtaacgg cgctgggaac ggacagtttt
    79861 agccagcaaa tgctggacgc ctggcacggc gagaacgttg atacttccct gacccaacgg
    79921 atggaaaacc gtctgccggg cctttactac attgaaaccg acagcaccgg tgaacgtacg
    79981 ttctactact ggcgtaacga agccgctgcc aaattctggc tggagagcga gcagtctgcg
    80041 gcgatttgcg aagagctggc gaatttcgat tatctctacc tgagcgggat tagcctggcg
    80101 atcttaagcc cgaccagccg cgaaaagctg ctttccctgc tgcgcgaatg ccgcgccaac
    80161 ggcggaaaag tgattttcga caataactat cgtccgcgcc tgtgggccag caaagaagag
    80221 acacagcagg tgtaccaaca aatgctggaa tgcacggata tcgccttcct gacgctggac
    80281 gatgaagacg cgctgtgggg tcaacagccg gtggaagacg tcattgcgcg cacccataac
    80341 gcgggcgtga aagaagtggt ggtgaaacgc ggggcggatt cttgcctggt gtccattgct
    80401 ggcgaagggt tagtggatgt tccggcggtg aaactgccga aagaaaaagt gatcgatacc
    80461 accgcagctg gcgactcttt cagcgccggt tatctggcgg tacgtttgac gggcggcagt
    80521 gcggaaaacg cggcgaaacg cgggcacctg accgcaagta ccgttattca gtatcgcggc
    80581 gcgattatcc cgcgtgaggc gatgccagcg taaacgcagc atatgcaaaa ggctggataa
    80641 agtgttaccc ctctatccag ctcatccata aatacctgat ggttgtgcta actcatcacc
    80701 atcaggcatt actgtgcagg tggaatatcc gtcacttcag gatgtacgtc atccgtggcg
    80761 acagaggtgg cggcagcagc agtcgatggg gccatgattt gatcccaggc cgcctgcaac
    80821 gctttcatgt taaattccgg ctcgcctttc ggctgcagca gtattaacgc catatcattc
    80881 gacaactgct gacgcagatc ctgatttaac atctccaccg tcaggctgtt aaggaaatcc
    80941 tgccgcagtt tctgatactg ctccggcgcg atattgacga cctgattttg caacgaacgc
    81001 atccgctgcc ccatcagaat atcggtatca gcccgcgcat aggcggcaaa cagtttctgc
    81061 agctccagtt tcttttgcgc cactaacgca ttgaactctt cttccggcag acctttatcg
    81121 cgaaccttcg ccagttcacg cgccaccaga ttaaggttgc tgttcagctt gtcgtttggt
    81181 gattcgatgt tgatggcaca ctgcgcacgc agatacagca cacggcagtc aaatccaaga
    81241 ccgatgtctt tgctgttact ggcgcttaac gcttgctgaa catgccagaa cagcgcctcg
    81301 cgggccaggt ccgcacgcca gtagcgcagc agtgcggctg attcacgaat cggctgccac
    81361 ggcgtatccc acatgataga taaccggtcc tgacgcaccg cgtcagtcat aatactcacc
    81421 gcttccgcac gcagcggaga aagcgtcggc accggagctg gcgtttcacg tttgcctttc
    81481 agttcgccaa acgttttgtt gatttggtcg acaaccgagc gcgcatccac gtttcccacc
    81541 accagtagcg tcattgcatc cggggtgtac catttctgat agaaatcttt aattttttcc
    81601 gcttcaacgg gttgtttcag cggatcggca ggatcgtgac ctaacaaggt tgatcctttc
    81661 agacgataac gccaccagcc ctctttagta tcggcaggcc aggttgccac catgtcctga
    81721 ctttgcagcg cgtggttgat ggtttctggc gtgatagtca gtttgccagt ggcatttgcc
    81781 aggtaagaga gcgcctcttt cagcaaatcg ttacggttat tgggcaaact cagattaaac
    81841 agcgtagtgt catacgagac aattaccggc ggcatcgggc gtttagggtc gatcccctgc
    81901 tgccacaatg aacgcgcctg tgctgcgtca aggccaccgc tttgcgttag cgcaatacga
    81961 gggatggcgt gactgtaacc gctctgttgt gtactttcgg cgagcgaacc ggtattaacc
    82021 agcaggcgaa tttcaacacg atcgctggga cgctgggggg tggtcagcac ttgccactgt
    82081 aaaccgttgg aaagcgtccc ctgttgccat gctggatcag gctggagcgc atctgcctgc
    82141 acatagccag cagtggccat catcagcaaa ccgcccgcta aaagtcgaat ttttgtgccc
    82201 tgcatgtgaa cccctgatca actatcctgg taataaaaaa actgcccgtt gtcggtcagt
    82261 ctaaatgacg ttaaaaacac ttcgtgttag accgcaagaa catgaaaatg tcacggaaga
    82321 agtgaaataa acctgaactc acccaggtgg taagttcagg cacaggggtc aattatgcgc
    82381 aaacacccgc actcggggaa gggagtgcgg gcataagtga tgagattaag aggataattc
    82441 gtgcgttttg ccatccggcg cacgattatt cagcacatcg tccagttttt tgtggtccag
    82501 ttctttcacc cacttagcaa cgacaatggt cgctacgccg ttaccgacca ggttagtcag
    82561 cgcacgagct tctgacataa agcggtcgat accgaggatc agcgccagac ccgctaccgg
    82621 caaatggccc accgcagaga gcgtcgccgc cagcacgata aagccactac ccgttacccc
    82681 tgccgcccct ttagaagaaa gcagcaacac gattaacagc gtgatttggt ggacgatatc
    82741 catctgactg ttagtggcct gggcgataaa caccgccgcc attgtcaggt atatcgatgt
    82801 gccatcaagg ttaaacgagt agcctgtcgg gatgaccagc cccaccaccg atttgcggca
    82861 gccgagtttc tccatcttgt cgagcatacg cggcagcgcc gactcggaag atgaagtccc
    82921 cagtacaatc agcagttctt cacggatgta gcggataaat ttgaagatac tgaaaccagt
    82981 cgctttagcg attgaaccca ataccagcac cacaaacagg atacaggtaa tgtagaaaca
    83041 gataatcagc tgccccagtt gcaccagtgt gccgacgccg tatttaccga tggtaaacgc
    83101 cattgccccg aacgcaccaa taggcgccag acgcatgatc atattgatga tgccgaagat
    83161 gacctgcgag aaactttcga tgacgttaaa aatcagttgg cctttgctgc ccagacggtg
    83221 gagcgcaaaa ccaaacagta cggcaaacag cagcacctgc agaatgttac cgctggcaaa
    83281 tgcgccaatg acgctcgccg ggatgacatc cataatgaag gcgacaatgc cctggtcttt
    83341 cgcctgatcg gcgtaaaccg ctaccgcttt cgcatcaagc gttgccggat cgacgttcat
    83401 tccggcacca ggctgcacga cgttaacgat gataagacca ataatcagcg cgatggtact
    83461 gacaatttca aagtaaagca gtgcgactgc gccggtacga ccgaccgcct tcatgctttc
    83521 catgcccgca atgcccgtta cgacggtaca aaagatgaca ggagcgatga tcatcttaat
    83581 gagcttaacg aagccgtcgc caagcggttt catttgctcg cctatttcag gatagaaatg
    83641 gccaaggaga ataccaatgg ctatcgctgt caggacctga aagtaaaggc ttttaaacag
    83701 agaggttttc atagggtgtc ctttagtaaa accacaggtc ttgtaaggtt atggggtacc
    83761 tgcggcctta aaataacacc cagacaacat cacagaaatg tacctggatc ataattgaaa
    83821 caaaaaggtt aaaaagtttg agctggctcg cacaaaccag cactttttaa agttttgtaa
    83881 tcagtttggg gtagctactt ttcttccagg taactctctt cgaagatttc aatagggagt
    83941 gggcgagcaa aaaggaagcc ctgggcaata ccaacgcccg cttttgccag ccagtcgcgt
    84001 tgtgcttcag tctccacgcc ttcggcaatc atttgtaagt tcaggctctg cgccagcatg
    84061 ataattgcag caatcatgct gctatcttcc ggcaagcctt caacaaacat tttgtcgatt
    84121 ttcagtacgt cgattggcaa cgatttcata tgctgcagct gacgcagccc tgcgtagccc
    84181 atgccgaaat catccagcgc cacccgaact ccggcattgc gcagcggacg gaggattgcc
    84241 accgcagcat gagggtcgtc aatacgtcgg ctttctgtca cttccagaat cagtgttccc
    84301 ggctgaatgc gatagcgggt taacagttcc agcatatccg ccaccatatt cgggtgcatc
    84361 agttgcagcg cagagaggtt taccgacaag g
//
LOCUS       DAEVZA010000004        78964 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEVZA010000004 DAEVZA010000000
VERSION     DAEVZA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.004"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(52..1386)
                     /gene="srmB"
                     /locus_tag="LAF92_000266"
     CDS             complement(52..1386)
                     /gene="srmB"
                     /locus_tag="LAF92_000266"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBI9789329.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            1518..2255
                     /gene="trmN"
                     /locus_tag="LAF92_000267"
     CDS             1518..2255
                     /gene="trmN"
                     /locus_tag="LAF92_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBI9789330.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(2240..3862)
                     /gene="nadB"
                     /locus_tag="LAF92_000268"
     CDS             complement(2240..3862)
                     /gene="nadB"
                     /locus_tag="LAF92_000268"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBI9789331.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            4118..4273
                     /gene="rseD"
                     /locus_tag="LAF92_000269"
     CDS             4118..4273
                     /gene="rseD"
                     /locus_tag="LAF92_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBI9789332.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            4270..4845
                     /gene="rpoE"
                     /locus_tag="LAF92_000270"
     CDS             4270..4845
                     /gene="rpoE"
                     /locus_tag="LAF92_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBI9789333.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            4878..5528
                     /gene="rseA"
                     /locus_tag="LAF92_000271"
     CDS             4878..5528
                     /gene="rseA"
                     /locus_tag="LAF92_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBI9789334.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            5528..6484
                     /gene="rseB"
                     /locus_tag="LAF92_000272"
     CDS             5528..6484
                     /gene="rseB"
                     /locus_tag="LAF92_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBI9789335.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            6481..6960
                     /gene="rseC"
                     /locus_tag="LAF92_000273"
     CDS             6481..6960
                     /gene="rseC"
                     /locus_tag="LAF92_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBI9789336.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            7158..8957
                     /gene="lepA"
                     /locus_tag="LAF92_000274"
     CDS             7158..8957
                     /gene="lepA"
                     /locus_tag="LAF92_000274"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBI9789337.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            8973..9947
                     /gene="lepB"
                     /locus_tag="LAF92_000275"
     CDS             8973..9947
                     /gene="lepB"
                     /locus_tag="LAF92_000275"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBI9789338.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            10220..10900
                     /gene="rnc"
                     /locus_tag="LAF92_000276"
     CDS             10220..10900
                     /gene="rnc"
                     /locus_tag="LAF92_000276"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBI9789339.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            10897..11802
                     /gene="era"
                     /locus_tag="LAF92_000277"
     CDS             10897..11802
                     /gene="era"
                     /locus_tag="LAF92_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBI9789340.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            11814..12542
                     /gene="recO"
                     /locus_tag="LAF92_000278"
     CDS             11814..12542
                     /gene="recO"
                     /locus_tag="LAF92_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBI9789341.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            12554..13285
                     /gene="pdxJ"
                     /locus_tag="LAF92_000279"
     CDS             12554..13285
                     /gene="pdxJ"
                     /locus_tag="LAF92_000279"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBI9789342.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            13285..13665
                     /locus_tag="LAF92_000280"
     CDS             13285..13665
                     /locus_tag="LAF92_000280"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBI9789343.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            14085..14165
                     /gene="shoB"
                     /locus_tag="LAF92_000281"
     CDS             14085..14165
                     /gene="shoB"
                     /locus_tag="LAF92_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBI9789344.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(14359..14619)
                     /gene="yfhL"
                     /locus_tag="LAF92_000282"
     CDS             complement(14359..14619)
                     /gene="yfhL"
                     /locus_tag="LAF92_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBI9789345.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            14834..16231
                     /locus_tag="LAF92_000283"
     CDS             14834..16231
                     /locus_tag="LAF92_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI9789346.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            complement(16228..16428)
                     /locus_tag="LAF92_000284"
     CDS             complement(16228..16428)
                     /locus_tag="LAF92_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI9789347.1"
                     /translation="MSEKPDDLLTPDEVCQKLGITQKTLCEWNIKHRHRAILAPIRFS
                     AKVVRYERRNVDAFIQKCRSQY"
     gene            complement(16425..17816)
                     /locus_tag="LAF92_000285"
     CDS             complement(16425..17816)
                     /locus_tag="LAF92_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI9789348.1"
                     /translation="MITFKPRPYQDLIINHQIETLRGNIWAGMGMGKTVATLTTLEDL
                     FMAGAETRPALVLAPLRVAASTWPDEALKWKHLRNIEVQPIVGNVKARSTALANSNAS
                     VFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVRR
                     WINLTGTPAPNGLVDLWGQSWFVDLGQRLGRTYGAFTSRWFKSIQFPGQSWTKLEPFA
                     HSQGEIQRALADVTLSLDATDWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELNG
                     EGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVAY
                     HWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNILV
                     FFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTVQ
                     DILLDAMKKRGIA"
     gene            17855..18565
                     /locus_tag="LAF92_000286"
     CDS             17855..18565
                     /locus_tag="LAF92_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789349.1"
                     /translation="MNVKDLSITTVLLAALYFMAFSFYKGYSSFYGFPVSFISIGIEE
                     IVKFSVIALGLLCTLVALLHIDTEEKNIPWGVYLFFFILASLLSYWTMYLYGGSSYLY
                     EKSIRDVATQAILLGFISPISVRAVSVFIRNEFRIKNKTHGVMFFISIAMLPSVLGWA
                     WAYLSDEPLFYSKKYDAYIIESYNGKFVLGNCKKNSSEFMSVEGIEGKLIPVSIKETQ
                     QLKVCFLKASKLKKNRSE"
     gene            complement(18571..18840)
                     /locus_tag="LAF92_000287"
     CDS             complement(18571..18840)
                     /locus_tag="LAF92_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBI9789350.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGHRSVPDRIVLLPG
                     GRIVFVECKSPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            complement(18830..19129)
                     /locus_tag="LAF92_000288"
     CDS             complement(18830..19129)
                     /locus_tag="LAF92_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HBI9789351.1"
                     /translation="MIVYVAGPMSGYEQFNRPAFHSAAKRLTDKGYVVLNPATLPDGL
                     TQAQYMDICLAMLRCADTIYMLKEWEYSAGARAENALAEKLELNVLFEEWDSDGL"
     gene            complement(19126..19341)
                     /locus_tag="LAF92_000289"
     CDS             complement(19126..19341)
                     /locus_tag="LAF92_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBI9789352.1"
                     /translation="MSFKYRDNPLYYRAAREALRLEQSGEYDRAAKVWAKANRESHHE
                     LNQEWSERRSDFCLMQNMREKRKVVEE"
     gene            complement(19347..21410)
                     /locus_tag="LAF92_000290"
     CDS             complement(19347..21410)
                     /locus_tag="LAF92_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBI9789353.1"
                     /translation="MSNILWGDLETYCDIPINNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIADPETLLYFHNSHFDRTVLRYTMPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDMQLARAAIEAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPDLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYDESVKRKSTYGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEKGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITEAPDTEDFNDKALSALLSTNPEWAPDIPLNAGGFEAY
                     HYRKD"
     gene            21472..22161
                     /locus_tag="LAF92_000291"
     CDS             21472..22161
                     /locus_tag="LAF92_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789354.1"
                     /translation="MTVGNKRQPNGLPEYYCHRGNDIKDAIVRDFIIQPVAHLKLITG
                     QTKTSYSGYPLTDQYYCFSFKKRSDENCTGAFFCGRDVAKDFIRRIGCTELPLFNPMK
                     SDTSAAIERSGSGSGSSGITVKPNADKKLFIDAARLLMTLWDSTSKGQTPLASVLNEA
                     ISGGIDKPPEIRHVVAVNTMLGNTKNTLTGLLDKARAKYPDVLFRDFDFTPLNEIVKR
                     SKSYNKHPPCY"
     gene            complement(22155..22796)
                     /locus_tag="LAF92_000292"
     CDS             complement(22155..22796)
                     /locus_tag="LAF92_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789355.1"
                     /translation="MSQPVLTNHLKTQIINNALEKAGIPKRKSALRAARVEWAERVRL
                     AAIGGVEKEAEILKNIKKIESMVTKIPDSLKTNSSLIRKDCCIYLNLAGARANIYFNG
                     NYRGYESGAPEHINKIAPAEFTLLGEDPLVTEFYSFDALYTQIKSDETEIIQNVSAAL
                     NKVRTVKRLLDVWPEAKELLPTDAPPVPLAPAVHRETLNEMIGLPTNTVSDTQ"
     gene            complement(22848..23396)
                     /locus_tag="LAF92_000293"
     CDS             complement(22848..23396)
                     /locus_tag="LAF92_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBI9789356.1"
                     /translation="MKLKLNNVRLAFPSLFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(23412..24713)
                     /locus_tag="LAF92_000294"
     CDS             complement(23412..24713)
                     /locus_tag="LAF92_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBI9789357.1"
                     /translation="MPDVHAQLSPSSAHRWMRCPGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGERAQEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWIDEEAARALLKDQFRYKTEEVFDFKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLTADLI"
     gene            complement(24716..25618)
                     /locus_tag="LAF92_000295"
     CDS             complement(24716..25618)
                     /locus_tag="LAF92_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789358.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATETENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            complement(25615..25764)
                     /locus_tag="LAF92_000296"
     CDS             complement(25615..25764)
                     /locus_tag="LAF92_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789359.1"
                     /translation="MKPEHLYRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            complement(26398..27021)
                     /locus_tag="LAF92_000297"
     CDS             complement(26398..27021)
                     /locus_tag="LAF92_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI9789360.1"
                     /translation="MSLAARFKARRLELGMTQVEVANSAGVSQQSIESIESGRTRKPR
                     NLLDLAKALKCSPDWLLNGKNIMPLAEISTRRIPILSYVQAGELTEARDITDLTGEFE
                     YILADSDIPETCFALRIDGDSMQPEFKEGDIVIIDPDLCPAPGEFVVAKNDGHEATFK
                     KYRPLGIGIDDFELIPLNPDYPIFRSADMNLQIIGVMIEHRIYRRKR"
     gene            27142..27354
                     /locus_tag="LAF92_000298"
     CDS             27142..27354
                     /locus_tag="LAF92_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9789361.1"
                     /translation="MTLATRVKERRKELKMTQVTLAELTGVSQQAINRIESGVIARPR
                     YLLEMSIALDCDPNWLLYGSQNDKKA"
     gene            27358..29550
                     /locus_tag="LAF92_000299"
     CDS             27358..29550
                     /locus_tag="LAF92_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBI9789362.1"
                     /translation="MSDSKPWGATPDEWFHFDLVLGRTDHLLPVVCNPGATISPDSKL
                     KALGKTPSRYNRDRQVTGIAQWTGHVVTEHDFARWSNEPDYGICVRTGHGWLALDCDS
                     EDEDIQADIRKTLVQLLGELPPRRWRANSNKCLYLLAVDGDFRKRIHRLAGDMGIIEL
                     LANGQQFVACGTHSSGARIEWDGGLPDEPPAITGEQLETLWQRLAEQLPVSVTTEAGN
                     TKMRDRSAFTPGATDDTAEYLDANGWTLLDGANGERYIRCPFEDGHSSGGDPTSTVYF
                     PAGTAGFEQGHFKCLHASCAHRDDGDFLNAIGIRNDDFEDLTSTEVAEPLPLPAFERD
                     KWGRIEATISNAAKAVVRPDFVDIDIRFDLFRDEIMFAPAGSGQWRAFTDADYARLRI
                     TMEKRGFKPVGRELIRDVVLLAADEQPFDSAIAWLNGLEWDGVPRIECFYHTHFGTAD
                     TPYTRAVSMYMWTALAGRVLEPGIKADMVPILVGPQGCGKSSGVEALSPDPAFFTEIS
                     FAEKDDDLARKMRGRLVAEIGELRGLNTKELESIKAFVTRTHENWIPKYREFATQFPR
                     RLVFVGTTNEDEFLADKTGNRRWLPVEVSKVDVKAIKRDLLLLWAEAREVFQRLGGIQ
                     FREAEQLAASVHEQYTIKDAWLETVEKWLDTPDLMTNELPRNCEFLRASDVLRDAIGL
                     NPDRIGKREEMRISNVLQNCGYKRAQRRIGGKMTRIFEAVSQPVPASK"
     gene            29832..30254
                     /locus_tag="LAF92_000300"
     CDS             29832..30254
                     /locus_tag="LAF92_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBI9789363.1"
                     /translation="MRNIQQVLERWGGWAASEGGSVYFPPVAAGFKNLLPATQSGRLK
                     CSDNDGLIINSAMSCLKKKDPYLCTLLEWHYVQAMPVRAMGEKLGVSHTHVLKRLQAA
                     EGFIDGCLAMLDVVLEMDQSVQSKPQAIRTLRRSCSAA"
     gene            30286..30627
                     /locus_tag="LAF92_000301"
     CDS             30286..30627
                     /locus_tag="LAF92_000301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789364.1"
                     /translation="MALISVRNRFESFMEQRYPDLSLQVKGNISASMKAQLGIRIERE
                     LYSEDVTYCDSAVQLMWTLFQAGVLAERRATSVTLPALKAKPDSFYDAGYNEGIQDCR
                     KHLTASGIKVR"
     gene            31073..31414
                     /locus_tag="LAF92_000302"
     CDS             31073..31414
                     /locus_tag="LAF92_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HBI9789365.1"
                     /translation="MKMHNAPHSWPDLLELLQSWWRGDTPLGAVVMSIVMAGLRIAYF
                     GGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGGSKE"
     gene            31418..31894
                     /locus_tag="LAF92_000303"
     CDS             31418..31894
                     /locus_tag="LAF92_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBI9789366.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLISRFKDAGGVVNEADL"
     gene            31878..32402
                     /locus_tag="LAF92_000304"
     CDS             31878..32402
                     /locus_tag="LAF92_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789367.1"
                     /translation="MKLTYKIVIAAFFFSAFGALVWSANHYHSKYRAEKLRADKAEGE
                     AEYQGKVIANQALNFNRFNQIAENASRLNSLVDIGHEKTVIKYREVLLREKNCDFPVP
                     VDIAVGVLNYANRLRASALHADSGDIDSAGDRATTTRTLTYCQAVLWINPLLAAIEKA
                     NNQLAGVRQVEQSR"
     gene            32464..33036
                     /locus_tag="LAF92_000305"
     CDS             32464..33036
                     /locus_tag="LAF92_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBI9789368.1"
                     /translation="MTKKLKAKHEVFCREFLVDLNATQAAIRAGYASRRAHVTGAELY
                     GKPEIRARINELKQERIDQLDIDANYVLMRLVEIDRLDVADILEDDLSIKPLSAWPES
                     WRRYLSGFNLAEMFEGRGDDREMVGILKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            33039..34661
                     /locus_tag="LAF92_000306"
     CDS             33039..34661
                     /locus_tag="LAF92_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HBI9789369.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWSNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWSEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDEAAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNAF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAVRRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMAYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMINYAPMNSALNIWELLGRQA"
     gene            34661..36127
                     /locus_tag="LAF92_000307"
     CDS             34661..36127
                     /locus_tag="LAF92_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBI9789370.1"
                     /translation="MARNKQALRRTAQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMIAPVLTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPLSNQPTQDSAGGRGHRKWSLRWFK"
     gene            36018..36752
                     /locus_tag="LAF92_000308"
     CDS             36018..36752
                     /locus_tag="LAF92_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBI9789371.1"
                     /translation="MTNRRNRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIE
                     ALERYSEIITPWATKVAENFTADIVRKNDEQWRKHSKTISRELRNLVNSAPPGQVMKS
                     IVAEQVKYIKSLPLEAADRVYDIQNRAIDAVVTGGRAEHFAKEIAASGDIAKSRADLI
                     ARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDGM
                     TGHAGELPNCRCYKEIVFPTSHSYPA"
     gene            36767..37987
                     /locus_tag="LAF92_000309"
     CDS             36767..37987
                     /locus_tag="LAF92_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBI9789372.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITVLHPEDENGDVRLVNPENWKELAVGH
                     LQNVRRGAGVQSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYKQAEPGKAQQVDI
                     TGNHVALVSKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRATTGDAQRPNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            37991..38497
                     /locus_tag="LAF92_000310"
     CDS             37991..38497
                     /locus_tag="LAF92_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789373.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGSGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
     gene            38509..39450
                     /locus_tag="LAF92_000311"
     CDS             38509..39450
                     /locus_tag="LAF92_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBI9789374.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETLSYVDGI"
     gene            complement(39447..39680)
                     /locus_tag="LAF92_000312"
     CDS             complement(39447..39680)
                     /locus_tag="LAF92_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789375.1"
                     /translation="MFYLVTRVCGFANGYFRVRTSEPVRGNVFLHSMTAFNKLAPRTV
                     GKREHKRGMYVYYCIIFCHRVILNGPARGPYGC"
     gene            39679..40086
                     /locus_tag="LAF92_000313"
     CDS             39679..40086
                     /locus_tag="LAF92_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HBI9789376.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            40083..40637
                     /locus_tag="LAF92_000314"
     CDS             40083..40637
                     /locus_tag="LAF92_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789377.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSERDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKTAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            40624..41013
                     /locus_tag="LAF92_000315"
     CDS             40624..41013
                     /locus_tag="LAF92_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HBI9789378.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVEKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            40988..41551
                     /locus_tag="LAF92_000316"
     CDS             40988..41551
                     /locus_tag="LAF92_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789379.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPAETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            41555..42700
                     /locus_tag="LAF92_000317"
     CDS             41555..42700
                     /locus_tag="LAF92_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HBI9789380.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDADIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMTNGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQTEAGITRLLSSVEKSLDQAVQNGLIAPGVWNGGDLGQLSSGDTLP
                     KGYYVYAQPLDEQAQSEREARKAPVIQAAIKLAGAVHYADVQINVVR"
     gene            42711..43151
                     /locus_tag="LAF92_000318"
     CDS             42711..43151
                     /locus_tag="LAF92_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HBI9789381.1"
                     /translation="MSTYSFMDVTATLTGPTGSIDLGYGSASSEEGIVVAMGGPKNTM
                     TIGADGEVMHSLHADKSGTITVNLLKTSPTNKKLSLAYNAQSQSSATWGNNVIVIRNK
                     VSGDIITARSVAFQKQPDNANAKTGNTMPWVFDCGKIDQVLGEF"
     gene            43155..43607
                     /locus_tag="LAF92_000319"
     CDS             43155..43607
                     /locus_tag="LAF92_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789382.1"
                     /translation="MEFEIKGVKYRVAKLSVFDQLKVTRKLLPVLAGMMSDFGSIRSR
                     LPADGKIDTVKFEQLKPVFETMLPRIAEELSSLTEDDTDAIIHPCLAVVSRRHMDGWV
                     PVFTRGELMFDDIDLLVMLQLVARVVADSLGNFLPTPLTSTTQSLQQG"
     gene            43785..45773
                     /locus_tag="LAF92_000320"
     CDS             43785..45773
                     /locus_tag="LAF92_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HBI9789383.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GAALSVVAFTAKIASGLDNLYWASQRTGATVQGIQSIGYAVSQVGGSVDAARSSLESL
                     SRFIRNNPGAEGFLNRLGVQTRDASGNMRDMAAIFTGVGQKLSSMPYYRANQYAQMLG
                     IDENTLMAMRRGVGGFSGQYSAMAKAIGFNADEAARSSNKFMTSLREFSAMAGMARDK
                     IGSNLAGGLAGSLDTLRRHILDNFPRIEQTLTKAIKGILALGDIIGRLFFRLIEGTSG
                     LITWWQSLDKQTRELISLFGALTIALRILNSTFWMSPVGLITALAAGIALLWEDYQTW
                     KEGGDSLIDWGKWKPEVDAALKMVRDLKTTVNDLAKALAKLLNIDPKSWSLKWDFSNF
                     IDQMGEFSKMLNMIADLLNAIKDGRWADAVSIGKQILNQGSENPSAMPMVTDSANSTA
                     DWIKEHWGFDPRSVGRTVRGWFGDDEPEQHAQATKRGERNNNPGNLNFAGQAGASLER
                     PGGRFARFETAFDGLRALARQLMLYAGRGINSVEKIISTWAPASDNNNTTAYIRAVSQ
                     RLGVDPRAALNMSDPQTMSALMSSIIQHENGRNIYSRELINKAAVAGISGKMTEVNQQ
                     NTYHIYGGGDPHAVGNEVARRQQSANAQVMRSNQVRVG"
     gene            45773..46360
                     /locus_tag="LAF92_000321"
     CDS             45773..46360
                     /locus_tag="LAF92_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789384.1"
                     /translation="MDILSTLFHQQSRKIGMIVPSVVISEKHTDMLEITEHPVEVGAA
                     VADHAYKKPSEVVMEVGFAGGGALLDFASNLTATSLLGLSPQQTYQELLDLQENRIPF
                     DVVTGKRLYSNMLIRALEVTTDKTTENVLSAVLTLREVIISRTQQITVADKTNMKEGA
                     STSSVQNSGNKTTKPPDTSLLKSITGNVASLLGGG"
     gene            46360..46662
                     /locus_tag="LAF92_000322"
     CDS             46360..46662
                     /locus_tag="LAF92_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789385.1"
                     /translation="MTIQEIPLTADNQQFSIVLGGVTWRISIIWRDLYWIMDLQNDRG
                     EPVISGIPLVTGADLLAQYACMELGFKLVVVCDDNTQDYPTKTDLGGRSHLLVSTE"
     gene            46665..47726
                     /locus_tag="LAF92_000323"
     CDS             46665..47726
                     /locus_tag="LAF92_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789386.1"
                     /translation="MSQNWMRHFELQLVDGNGQGIELSDFKVTFTIDWFNISSASRVG
                     TIKIYNLSADTVNRITGQEFSKVRLIAGYDGIAPEVSASDVGTVREVDAADVGQRDGR
                     NYGLIFSGEIRYSVTGKDSPVDTYVLIQAADTDLAFATSITSQTLAAGYTVADVNRAL
                     MKDFEAKGATEGLTPEMPATVFPRGRVLFGMTRHLMDNVAGQCGATWQFVDGQRQMVA
                     NNEYVHEAIVLNSATGLIGMPQQTIGNGVNVRALINPNIRVNGLIQLDQASVYRTALS
                     NNDIAMVGGQITDQNTDGNITLSGTTSQPASIATDGVYIVRGIMYTGDTRGQAWYMDM
                     MCEARGAADLYTQSALQRG"
     gene            47730..48074
                     /locus_tag="LAF92_000324"
     CDS             47730..48074
                     /locus_tag="LAF92_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789387.1"
                     /translation="MRGIIFLLAAFSACSAWADGFTVKCGGYTMVANQGELSTINGER
                     VTSQKITELGTNGLKVDMGLMPAKDGNNYGFEYIRRPGTETRFLNVQLLQNSMDAPKI
                     IGSFPCKKVAGR"
     gene            48400..48972
                     /locus_tag="LAF92_000325"
     CDS             48400..48972
                     /locus_tag="LAF92_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HBI9789388.1"
                     /translation="MSSKPRKQAAVLKKHQENKASQKAVPDSDGIDEDIDSSEYDESQ
                     EVVEKLSAVANLDPELKSKLTPELLERVSKNPKVIAMFQQESFQGPLPPPAMLLQYNQ
                     ILPGGAERIFRLTEKEQEHRHKTQSDALLGAVSRDKRGQWMGFCIAMVVLIMAAFFAN
                     RGNTWFAGTLATLDLVGLTTVFVYGRKKPK"
     gene            49030..49782
                     /locus_tag="LAF92_000326"
     CDS             49030..49782
                     /locus_tag="LAF92_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBI9789389.1"
                     /translation="MAVSDQTRSGDLAETFKSERETTKNQIRVALPGIIQSFAPDAVT
                     AVVQPAIRYVEIDNDGNRITKNYPLLVDVPVVFPRGGGCTLTFPVKAGDECLVIFADR
                     CIDFWWQSGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDGVN"
     gene            49782..50135
                     /locus_tag="LAF92_000327"
     CDS             49782..50135
                     /locus_tag="LAF92_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789390.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETQGVKSILSFNTTVDTTTRRVMFSA
                     EIDTLYGITTVTSEA"
     gene            50135..51334
                     /locus_tag="LAF92_000328"
     CDS             50135..51334
                     /locus_tag="LAF92_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBI9789391.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDSNNVIWRLPASVVVGVDGTVMVTATCSVSGAVAALA
                     GTITEINTPTRGWVSVTNPAAATVGTPAETDAELRIRQSQSVALPSLTPFEALDGAVS
                     NVIGVTRHKLYENDTGSEDGNGLPPHSVAVIVDGGDVTDIAQAIRGNKGQGTATHGTT
                     SVTVPDKYGNPHVIKFSRSSDVPVYARIKLKVFTGYTSQIGQQIQQAISDYINSLTIG
                     DSVLLSRIYSPANLGVVSGGNARYYDIQELTIGKSPGALSSSNIDIRYNESASCTPEN
                     IVITVES"
     gene            51331..52011
                     /locus_tag="LAF92_000329"
     CDS             51331..52011
                     /locus_tag="LAF92_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBI9789392.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITVFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFRWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGLRMQIVDNQDMTISVWVFPDT
                     DISDVSRELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     LGAWGVLL"
     gene            52011..52856
                     /locus_tag="LAF92_000330"
     CDS             52011..52856
                     /locus_tag="LAF92_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBI9789393.1"
                     /translation="MAKNDFKAFATGKNANVMSQEEWEALPALLSGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALENLGLGEGSALPVGAPVPWPSETPPTGWLKCNGAAFSAEEYPELAKAYPT
                     NKLPDLRGEFIRGWDDSRGIDTGRSLLSGQAATFIRTALQDYYGYDLNTNVKVGIAFA
                     TADSVITVGNPANPKAGNNSDYVPASADNSITGTQRTAEDNFTGAWISMRPRNLSFNY
                     IVRAA"
     gene            52856..53458
                     /locus_tag="LAF92_000331"
     CDS             52856..53458
                     /locus_tag="LAF92_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI9789394.1"
                     /translation="MDNAVLNSEFIATKAGDITVYNYNGETREYISTSTEYLAVGVGI
                     PACSCLDAPGTYKAGYAICRSADLNSWEYVPDHRGEIVYNTETGDAKEITTPGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQPENTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(53430..53873)
                     /locus_tag="LAF92_000332"
     CDS             complement(53430..53873)
                     /locus_tag="LAF92_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI9789395.1"
                     /translation="MMKYLYANNLAYPYELQSLYVEKGEWPEEKGVDIDEVIFREYFY
                     DTPPEGKYRCVGEDGLPAWADIPPPTREEQIASAETKKQQLINQANEYINSKQWPGKA
                     SIGRLKGEELAQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
     gene            complement(53873..54247)
                     /locus_tag="LAF92_000333"
     CDS             complement(53873..54247)
                     /locus_tag="LAF92_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9789396.1"
                     /translation="MMPVSRNLCIFLNVGLGEAAKRNVGTGENQIPDMSSYASGSGWR
                     KMPDGSIEQWGRISFPGEHGPVSANVSFPIPFTQTPGIVIVCDGGFGGGNMWGATNWS
                     TTGFIAHCNYGLEGGAFFAKGW"
     gene            54275..54670
                     /locus_tag="LAF92_000334"
     CDS             54275..54670
                     /locus_tag="LAF92_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI9789397.1"
                     /translation="MLIGYVRVSTNDQNTELQRNALECAGCELIFEDKISGTKSARPG
                     LKKLLRTLSEGDPLVVWKLDRLGRSVRHLVVLVEELREQGVNFSSYVITGGGFLPLMQ
                     IFSLTIGEVAIFFCFLKNYLQLYNCNNVH"
     gene            complement(54594..55439)
                     /locus_tag="LAF92_000335"
     CDS             complement(54594..55439)
                     /locus_tag="LAF92_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789398.1"
                     /translation="MNKNPWDIPAWHALGREASLVTQLIGSGATAIGKANYADKIGEY
                     YTAFFGLSVGFERLAKLILVAHYAIEHKGKMPDENIVRKFGHKLIELTNEVDSISKKM
                     QLSLRYSRPTHKITYKILECLDSFADARRGRYANFSSLSSPNLTNNEPINKWWGEVAE
                     SILEKHYYYTVEQRRVEENARFINATLSPYTTVMYMNESTNIMQDLISASIRTGQNTI
                     VQKWGRFYSLSIARWLATVLAELSDIASHKYGIISFYGLSEHCCSYIVEDSFLKNRKI
                     WPLRQ"
     gene            complement(56319..57167)
                     /locus_tag="LAF92_000336"
     CDS             complement(56319..57167)
                     /locus_tag="LAF92_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBI9789399.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLYECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            57376..58011
                     /gene="yfhb"
                     /locus_tag="LAF92_000337"
     CDS             57376..58011
                     /gene="yfhb"
                     /locus_tag="LAF92_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBI9789400.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            58036..58572
                     /gene="tadA"
                     /locus_tag="LAF92_000338"
     CDS             58036..58572
                     /gene="tadA"
                     /locus_tag="LAF92_000338"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBI9789401.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(58569..60125)
                     /gene="mltF"
                     /locus_tag="LAF92_000339"
     CDS             complement(58569..60125)
                     /gene="mltF"
                     /locus_tag="LAF92_000339"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBI9789402.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            60384..64271
                     /gene="purL"
                     /locus_tag="LAF92_000340"
                     /gene_synonym="purI"
     CDS             60384..64271
                     /gene="purL"
                     /locus_tag="LAF92_000340"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBI9789403.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILNDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            64847..66274
                     /gene="qseE"
                     /locus_tag="LAF92_000341"
     CDS             64847..66274
                     /gene="qseE"
                     /locus_tag="LAF92_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBI9789404.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            66438..67151
                     /gene="qseG"
                     /locus_tag="LAF92_000342"
     CDS             66438..67151
                     /gene="qseG"
                     /locus_tag="LAF92_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBI9789405.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTLAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            67141..68475
                     /gene="glrR"
                     /locus_tag="LAF92_000343"
     CDS             67141..68475
                     /gene="glrR"
                     /locus_tag="LAF92_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBI9789406.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            68536..68874
                     /gene="glnB"
                     /locus_tag="LAF92_000344"
     CDS             68536..68874
                     /gene="glnB"
                     /locus_tag="LAF92_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBI9789407.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(68919..70109)
                     /gene="hmpA"
                     /locus_tag="LAF92_000345"
     CDS             complement(68919..70109)
                     /gene="hmpA"
                     /locus_tag="LAF92_000345"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBI9789408.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKQEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            70438..71691
                     /gene="glyA"
                     /locus_tag="LAF92_000346"
     CDS             70438..71691
                     /gene="glyA"
                     /locus_tag="LAF92_000346"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBI9789409.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(71755..72948)
                     /locus_tag="LAF92_000347"
     CDS             complement(71755..72948)
                     /locus_tag="LAF92_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBI9789410.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            73066..76347
                     /locus_tag="LAF92_000348"
     CDS             73066..76347
                     /locus_tag="LAF92_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBI9789411.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGLQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            76444..77427
                     /locus_tag="LAF92_000349"
     CDS             76444..77427
                     /locus_tag="LAF92_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBI9789412.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            77450..78961
                     /locus_tag="LAF92_000350"
     CDS             77450..78961
                     /locus_tag="LAF92_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI9789413.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
BASE COUNT        19226 a        19126 c        21020 g        19592 t
ORIGIN      
        1 gttttgcgtt tgccagcact tttaatacca ggcttaaacc cggcattgag attactcttc
       61 tgtcgtttgc ggtggcacgc ccgttccgct tggtttacgg cgcttaccaa tatttttggt
      121 gtcgcgatgg cgttttttca cacgcggttt ctctttctct ttagcttttt tcttctcggc
      181 gcgttttgcc agtactttct tcgatggctt gccggtctgc ttctcgcttg gcgcacgcgt
      241 tttcgggcgt aactcatcaa taacgcgagc tttaatcggc tcttcaatgt aacggccaac
      301 cttgcccagc agcaaatggt catgggcttc caccagcgaa attgcggtgc ctttacgacc
      361 ggcgcgcgcg gtacgtccga tacggtgcaa ataagtatcg ccactgcgcg gcatatcgaa
      421 gttaaagacg tggctgacgt caggaatgtc gataccgcgc gcggcaacat cggttgcaac
      481 cagtacgttt acgcgacctt cggtcaaacg cttgatcgct tcgttacgct tgccttgtac
      541 catctcacct tcgagatagc agttgttgat gcccgcttcg cgcagccagt ttgccagctc
      601 atgcacacgt tcacgcttac gcacaaacac aattgagcgg gtcgcttccg gctgttttaa
      661 cagatgcacc agcaatgcgg ttttatgttc caggtcatcg gcgcggtaat accactgatg
      721 aatttttttg cgctcacggg tggacggatt ggcagaaacc tccaccggat cttccagcag
      781 acgctcggca aagtcctgaa tcgcatcgcc ttccagcgtt gccgaaaaga gcagggtctg
      841 tttacgccag cgcgtttcgc cagcaatatg ttcgatatcc tgagcgaagc ccatatccag
      901 catacggtct gcttcgtcga ggatcagcgt ttcaaccgcg cggcaatcga agttctcttc
      961 ttttatgtat tgcagcagac gtccggtcgt ggcgaccacg atgtcctgat tttcgctgaa
     1021 cacttccgcg tggttcatat aggctacgcc gccggtgatg gtggcgatat ccagatgcgt
     1081 atgtttcgcc agttcgcggg catgatcggc cacctgcatc gccagctcgc gagttggggt
     1141 gaggatcaaa atacgcggcg gaccggattt cttacgcggg aaatcgagca ggtgctgcaa
     1201 cgctggcagc agatacgccg ccgttttacc ggtgcctgtc ggcgcagaac cgagtacatc
     1261 acggccatcg agcgcaggcg gaatggcggc agcctgaatg gcggtcgggc gagtgaaacc
     1321 tttatcctgg agggcttcca gcaggctttc gtcgagttca agttcggaaa aagtcgttac
     1381 agtcatgttc tacctctgtg tggggcgctg attatagacg ttacggctgc aatcttcatc
     1441 tgtttgtatg gatatcgctt ctcgggtatt gttctcaccc ggtgctatcg ccgtttttcc
     1501 tgcaaggttt tattttcatg tcacagtcta catccgtgct tcgtcgtaat ggatttactt
     1561 ttaaacagtt ttttgttgct cacgatcgct gtgcgatgaa agtgggaacg gatggcattt
     1621 tgctgggcgc atgggcaccg gtggctgggg tgaaacgttg ccttgatatc ggcgcgggta
     1681 gcgggttgct ggcactgatg ctggcgcagc gaaccagtga cagcgtgata attgatgccg
     1741 ttgagctgga aagtgaagct gccgcgcagg cgcaggaaaa tatcaaccag tccccgtggg
     1801 cagagcggat taacgtccat acggcggata ttcagcagtg gatcacacag cagacagtac
     1861 gcttcgattt aatcatcagt aacccacctt actatcagca gggagtagag tgcgcgacac
     1921 ctcaacggga acaggctcgc tataccacta cgcttgatca cccatcgctg ctgacctgtg
     1981 cggcggagtg cattaccgaa gagggatttt tctgcgtggt gctgccagag cagattggta
     2041 atggttttac tgaactggca ttaagcatgg gctggcattt acgtttgcgg acggatgtgg
     2101 cggaaaacga agcgcgactg ccgcatcggg tgctgctggc attctccccg caggcgggag
     2161 aatgctttag cgatcgctta gtcattcgtg ggccagacca gaactattcc gaagcgtata
     2221 cggcgctgac ccaggctttt tatctgttta tgtaatgatt gccgggggaa aggatcgatg
     2281 gaccggaatg cgtgagcagt tccgggtaat ccagcgtaaa atgcagcccc cggctttctt
     2341 tgcgcatcat cgcgcagcga acaatcaact cggcaacctg taccagatta cgcagctcca
     2401 gcaaattatt tgagacgcgg aaatgggcgt aatattcgtc tatttcttgt tggagcatgg
     2461 ttatccgccg cagggcgcgt tccaggcgct tcgttgtgcg cacaatgcca acgtaatccc
     2521 acataaacag ccgtagctcg tgccagttat gctgaattac tacccgttcg tcagggttct
     2581 caacgcggct ttcatcccac ggcggtaacg tactgacgcc gtgggcatca ggcatacgtc
     2641 tggtgatatc ttctgccgcc gaccagccat agaccagaca ctccagcaat gaattcgagg
     2701 ccatgcggtt agcgccgtgt aagccggtat aactcacctc gccaatggca tacaagccct
     2761 cgacgtccgt acgcccatga tcatcaacca ttacaccacc gcaggtataa tgtgcagcag
     2821 gcacaatcgg taccggttct tgtgtgagat caatccccag cccgagcaat ttttcataaa
     2881 tcatcgggaa atgctggcga ataaaatcgg cgggcttatg gctgatgtcg aggaacatac
     2941 aatctgcgcc gaggcgtttc atttcatggt caatggcgcg ggcgacaata tcgcgcgggg
     3001 ccagttcgcc gcgctcatca aaatcgggca taaaacgcgt gccatccggg cgcttgagat
     3061 aagcgccttc gccgcgcagt gcttccgtta acaggaaatt gcgtgcctgt gggtgataca
     3121 gtgcggtagg gtggaactga ttaaattcga gattggcaac ccgacagcct gcgcgccacg
     3181 ccatagcaat gccatcgcca gaagaaatat ccggattggt ggtgtactga taaaccttcg
     3241 acgcaccgcc ggttgccagt accactgctt ttgcgtggca ggtttccacc gtttctttat
     3301 ttcggttcca tacccacgcg ccaacaaccc gtcgcgtgcc cgggaggcca attttgtcag
     3361 aaacaatcag atcaactgcg ttgctgcgct ccagcacacg aatattcgga tggttctgcg
     3421 ccttgctcac cagcgtggat tgtacttctc taccggtggc gtcggcggca tgaagaatac
     3481 gacggtgact atgtccacct tcacgggtca gatggtagct ttcttcgcca ttcggttgaa
     3541 cgtgggtatc aaacaaaacc ccttggtcaa ttaaccattg cacacaggat cgtgcattgc
     3601 tggcgacaaa ttcaactgca tggcgatcgc aaataccagc cccggcaatc aatgtgtctt
     3661 ccacatgcga gtcaatgctg tcagtttcat caaacacggc ggcaataccg ccctgggcat
     3721 aaaatgttga accttccgtt accgggcctt tacttagaac gatgacctga tgctggtcag
     3781 ccaggcgtag cgccagtgaa agtccggctg cgccgctacc gataatcaac acgtcacatg
     3841 aatgttctgg gagagtattc attttctttg tttaatttac taaacaccgt ttggtcagca
     3901 tagcatcatg ttgtgcgaat aaacacctgc tattttaata tttgttacag tggctaaaca
     3961 cgctgacgca gggcggcgag aaaaagagaa gttactggct ggtggaggat taggtggtga
     4021 aataaaaagg ccgttgggtt actcttcagg cagttaaatg ggcatttcta cacagataat
     4081 gcgatgttca gattctgtag acttataatg atagataatg atccgtctac agcatgacaa
     4141 acaaaaacag atgcgttacg gaactttaca aaaacgagac actctaaccc tttgcttgct
     4201 caaattgcag ctaatggagt ggcgtttcga tagcgcgtgg aaatttggtt tggggagact
     4261 ttacctcgga tgagcgagca gttaacggac caggtcctgg ttgaacgggt ccagaaggga
     4321 gatcagaaag cctttaacct actggtagtg cgctatcagc ataaagtggc gagtctggtt
     4381 tcccgctatg tgccgtcggg tgatgttccc gatgtggtac aagaagcttt tattaaagcc
     4441 tatcgtgcgc tggattcgtt ccggggagat agcgcttttt atacatggct gtatcggatt
     4501 gctgtaaata cagcgaaaaa ttacctggtt gctcaggggc gtcgtccacc ttccagtgat
     4561 gtggatgcca ttgaagctga aaacttcgaa agtggcggcg cgttgaaaga aatttcgaac
     4621 cctgagaact taatgttgtc agaagaactg agacagatag ttttccgaac tattgagtcc
     4681 ctcccggaag atttgcgcat ggcaataacc ttgcgggagc tggatggcct gagctatgaa
     4741 gagatagccg ctatcatgga ttgtccggta ggtacggtgc gttcacgtat cttccgagcg
     4801 agggaagcta ttgataacaa agttcaaccg cttatcaggc gttgacgata gcgggatact
     4861 ggataagggt attaggcatg cagaaagaac aactttccgc tttaatggat ggcgaaacgc
     4921 tggatagtga gctgcttaac gaactggctc ataacccaga aatgcagaaa acctgggaaa
     4981 gctatcactt aatccgtgac tcaatgcggg gtgatactcc cgaggtgctc catttcgata
     5041 tctcttcacg cgtgatggcc gccattgaag aagagccagt acgtcaaccg gcgacattga
     5101 tcccggaagc ccagcctgcg ccgcatcaat ggcagaaaat gccattctgg cagaaagtac
     5161 gtccgtgggc ggcacagctt acccaaatgg gcgtagcagc atgcgtatcg cttgcagtta
     5221 tcgttggcgt ccagcactat aatggacaat ctgaaacgtc ccagcagccc gaaacgccgg
     5281 tatttaatac actgccgatg atgggtaaag ccagcccggt aagcctggga gtaccttctg
     5341 aagcgaccgc aaacaatggt caacagcagc aggtacagga gcagcgtcgt cgcattaatg
     5401 caatgttgca ggattacgaa ctgcaacgcc gactccactc tgaacagctt cagtttgagc
     5461 aggcgcaaac ccagcaagcc gctgtacagg tgccaggaat tcaaacttta ggaacgcaat
     5521 cgcagtaatg aagcaacttt ggtttgccat gtcattagtg acaggtagcc tgttattctc
     5581 tgctaacgcc tcggccactc ccgcgtccgg ggcgttatta cagcagatga acctggccag
     5641 tcagtcactg aattacgagc tgtcattcat cagcatcaat aaacagggtg ttgagtctct
     5701 gcgttatcga catgcacgcc tcgataaccg tcctcttgca caattgttgc aaatggatgg
     5761 cccgcgccgg gaagtggtac agcgcggcaa tgaaatcagc tattttgaac cgggacttga
     5821 accgttcacg cttaatggcg attacattgt tgattctctg ccatcgctta tctataccga
     5881 tttcaaacgc ctttctcctt actacgactt tatctccgtc gggcgcacgc gtattgctga
     5941 tcgtctttgc gaagtcattc gcgtggttgc ccgagatggt acacgctaca gctacatcgt
     6001 gtggatggac accgaatcga aattaccgat gcgggttgat ctccttgatc gcgatggtga
     6061 aacgctggaa caatttcgcg tgattgcttt taacgtcaat caggatatca gcagcagtat
     6121 gcagacgctg gcgaaggcaa atttgccgcc gttgctttct gttcctgtag gtgaaaaagc
     6181 taaattcagc tggacgccaa cctggttgcc acagggtttt agcgaagttt ccagtagtcg
     6241 acgtccgcta ccgacgatgg acaacatgcc tatcgaatca cgtctctatt ccgacggatt
     6301 attcagcttc tcggtaaacg ttaaccgcgc tacgccatcg agcaccgatc agatgttgcg
     6361 caccggacgc agaaccgtca gtacaagcgt acgtgataac gccgaaatca ccattgtcgg
     6421 tgaactgccg ccgcaaacgg cgaaacgcat tgccgagaat attaagttcg gggcagcgca
     6481 atgatcaaag agtgggctac cgtcgtctcc tggcaaaacg ggcaggcgct ggtcagttgt
     6541 gatgttaaag cttcatgcag cagctgcgct tcacgcgccg gttgcggtag ccgcgtgtta
     6601 aataaacttg gcccgcaaac cacgcatacc attgtcgtac cctgtgatga gccgttagtg
     6661 ccggggcaaa aagtggaatt ggggatcgcc gaaggcagcc tgcttagctc cgcattactg
     6721 gtttatatgt cgccgctggt gggattattc ctcatcgctt cgctatttca gctactcttt
     6781 gcttctgacg tcgcagcatt atgtggtgcg gtcctcggcg gtattggcgg gttcctgatt
     6841 gcgcgcggct actcgcgtaa gtttgctgcc cgggcagaat ggcaaccgat catcttaagc
     6901 gtggcattgc cgcctggcct tgtgcgattt gaaacatctt cggaagacgc gagccagtga
     6961 tttcactggc tttgttgcat tttgcataac aaatgcgagg acgtttccag ataccacaat
     7021 ccatacggta tgaacatttc ctcgcctcaa tgttgtagtg tagaatgcgg cgtttctatt
     7081 aatacagacg ttaagctcag aacagcgact tctaaaagcc tggttaaccg ggcattaagg
     7141 cacaataatc atactttatg aagaatatac gtaacttttc gatcatagct cacattgacc
     7201 acggtaaatc gacgctgtct gaccgtatta tccagatctg cggtggcctg tctgaccgtg
     7261 aaatggaggc gcaggttctc gattccatgg atcttgagcg tgagcgtggc ataaccatca
     7321 aagcgcaaag cgtgacgctg gactacaaag cgtctgacgg cgaaacctat cagctgaact
     7381 ttatcgacac cccgggccac gttgacttct cctatgaagt ttcccgttcg ctggctgcct
     7441 gtgaaggtgc attgctggtg gtcgacgccg ggcagggcgt agaagcgcaa accctggcaa
     7501 actgctacac cgccatggaa atggatctcg aagtggtgcc ggtactgaac aagattgacc
     7561 tgccggcagc cgatcctgaa cgcgtggcgg aagaaattga agatatcgtc ggcatcgacg
     7621 ccaccgacgc ggtgcgctgt tcagcgaaaa ccggcgttgg cgtgcaggac gttctcgaac
     7681 gtctggtgcg cgacattccg ccgccggaag gcgatccgga aggcccgttg caggcactaa
     7741 ttatcgactc atggttcgac aactacctgg gcgttgtttc acttatccgt attaaaaacg
     7801 gcaccctgcg taagggcgac aaagtgaaag tcatgagtac cgggcagacc tataacgccg
     7861 accgtctggg catcttcacg ccgaaacagg ttgaccgcac tgaactgaaa tgtggcgaag
     7921 taggctggct ggtatgtgcg attaaagata tccacggcgc tccagtcggc gataccttaa
     7981 cgctggcgcg taatccggca gaaaaggcgc tgcctggctt taagaaagtc aaaccgcagg
     8041 tatacgccgg tctgttcccg gtaagttccg acgactatga agccttccgt gacgcgctgg
     8101 gtaaactcag cctgaacgat gcttcactgt tctatgagcc ggaaagctcc agcgcgctgg
     8161 gctttggttt ccgctgcggc ttcctcggcc tgctgcacat ggagatcatc caggaacgtc
     8221 tggaacgtga atacgatctg gatctgatca ccactgcgcc gaccgtagtg tatgaagttg
     8281 aaaccacgtc aagagaagtt atttacgtcg acagtccatc caagctgcca gcggtaaata
     8341 acatctacga actgcgcgag ccgattgccg aatgtcacat gctgctgccg caggcatatc
     8401 tcggcaacgt tattacgctg tgcgtagaaa aacgcggcgt gcagaccaat atggtttacc
     8461 acggtaatca ggtggcgctg acgtacgaga tcccgatggc ggaagtggtg ctcgacttct
     8521 tcgatcgcct gaaatctacc tcgcgtggtt atgcgtctct ggattacaac ttcaaacgct
     8581 tccaggcgtc cgacatggta cgtgtagacg tattaatcaa cggtgaacgt gttgatgcgt
     8641 tggcgctgat cacccaccgt gataattcgc aaaaccgcgg tcgcgagttg gtggagaaga
     8701 tgaaagatct gatcccacgc cagcagtttg atatcgccat tcaggcagcg attggtacgc
     8761 acattattgc gcgatccacc gtgaaacagc tgcgtaaaaa cgtactggct aaatgttatg
     8821 gcggcgatat cagccgtaag aaaaagctgc tgcagaagca gaaagaaggt aagaaacgca
     8881 tgaagcagat cggtaacgtc gagctgccgc aggaagcgtt cctcgccatt ctgcacgtcg
     8941 gcaaagacaa caaataaccc ttaggagttg gcatggcgaa tatgtttgcc ctgattctgg
     9001 tgattgccac actggtgacg ggcattttat ggtgcgtgga taaattcttt ttcgcaccta
     9061 aacggcggga acgtcaggca gcggcgcagg cggctgccgg tgactcactg gataaagcaa
     9121 cgttgaaaaa ggttgcgccg aagcctggct ggctggaaac cggtgcttct gtttttccgg
     9181 tactggctat cgtattgatt gtgcgttcgt ttatttatga accgttccag atcccgtcag
     9241 gttcgatgat gccgactctg ttaatcggtg attttattct ggtagaaaag tttgcttatg
     9301 gcattaaaga tcctatctac cagaaaacgc tgatcgaaac cggtcatccg aaacgcggag
     9361 atatcgtggt ctttaaatat ccggaagatc caaagcttga ttacatcaag cgcgcggtgg
     9421 gtttaccggg cgataaagtt acttatgatc cggtctcgaa agagctgacg attcaaccgg
     9481 gatgcagttc cggccaggcg tgtgaaaacg cgctgccggt cacctactca aacgtggaac
     9541 cgagcgattt cgttcagacc ttctcacgcc gtaatggtgg ggaagcgacc agcggattct
     9601 ttgaagtgcc gaaaaacgaa accaaagaaa atggaattcg tctttccgag cgtaaagaga
     9661 cactgggtga tgtgacgcac cgaattctga cagtgccgat tgcgcaggat caggtgggga
     9721 tgtattacca gcagccaggg caacaactgg caacctggat tgttcctccg ggacaatact
     9781 tcatgatggg cgacaaccgc gacaacagcg cggacagccg ttattggggg tttgtgcctg
     9841 aagcgaatct ggtcggtcgg gcaacggcta tctggatgag cttcgataag caagaaggcg
     9901 aatggccgac tggtgtgcgc ttaagtcgca ttggcggcat ccattaatag ctcatcttcg
     9961 ttcacgttgt cgccgttatg gcgacaacgt gaattattta tgagataaat ctcccgtggc
    10021 taacgacatc ccccgtcgtt gtgtatagaa tattcccccg aagtttaagg ttggcacctc
    10081 caggttgcca cggcacacga aacagcgttg gtccccatat accggtaaac tgaaactgca
    10141 gcgaagcagt tagcagagcc atgtatatca ggtctgtttc gtgtgctgaa ttgttgacgc
    10201 atttatttat tggtatcgca tgaaccccat cgtaattaat cggcttcaac ggaagctggg
    10261 ctacactttt aatcatcagg aactgttgca gcaggcatta actcatcgta gtgccagcag
    10321 caaacataac gagcgtttag aatttttagg cgactctatt ctgagctacg ttatcgccaa
    10381 tgcgctttat caccgtttcc ctcgtgtgga tgaaggcgat atgagccgga tgcgcgccac
    10441 gctggtccgt ggcaatacgc tggcggaact ggcgcgcgaa tttgagttag gcgagtgctt
    10501 acgtttaggg ccaggtgaac ttaaaagcgg tggatttcgt cgtgagtcaa ttctcgccga
    10561 caccgtcgaa gcattaattg gtggcgtatt cctcgacagt gatattcaaa ccgtcgagaa
    10621 attaatcctt aactggtatc aaactcgttt ggacgaaatt agcccaggcg ataaacaaaa
    10681 agatccgaaa acgcgcttgc aagaatattt gcagggtcgc catctgccgc tgccgactta
    10741 tctggtagtc caggtacgtg gcgaagcgca cgatcaggaa tttactatcc actgccaggt
    10801 cagcggcctg agtgaaccgg tggttggcac aggttcaagc cgtcgtaagg ctgagcaggc
    10861 tgccgccgaa caggcgttga aaaaactgga gctggaatga gcatcgataa aagttactgc
    10921 ggatttattg ccatcgtcgg acgtccgaac gttggcaaat ccacattgtt gaacaaactg
    10981 ctggggcaga agatctccat cacctcccgc aaagcgcaaa caactcgtca ccgcattgtg
    11041 gggatccata ctgaaggcgc gtatcaggcg atctacgttg atacccctgg cctgcatatg
    11101 gaagaaaaac gcgctattaa ccgcctgatg aacaaagcgg cgagcagctc tattggcgac
    11161 gttgagctgg tgatttttgt cgttgaaggc acccgctgga cgccggacga cgaaatggtg
    11221 ctcaacaaac tgcgcgatgg caaagcgccg gtgatcctcg cggtgaacaa agtggataac
    11281 gtgcaggaaa aagccgatct gctgccgcac ttacagttcc tggcaagtca gatgaacttc
    11341 ctcgatatcg tgccgatctc tgctgaaacc gggctgaatg tcgacactat cgcagcaatc
    11401 gtgcgtaagc atctccctga agcgacccat cacttcccgg aagattacat caccgatcgc
    11461 tcacagcgtt ttatggcgtc tgaaatcatc cgcgaaaaac tgatgcgttt cctcggcgcc
    11521 gaactgccgt actccgtgac cgtggagatc gaacgtttcg tctctaacga acgcggtggt
    11581 tatgacatca atggtttgat tctcgttgag cgtgaagggc agaagaagat ggttattggc
    11641 aacaaagggg ccaagatcaa aaccatcggg attgaagcgc gtaaagacat gcaggaaatg
    11701 ttcgaagcgc ctgtccacct tgaactgtgg gtaaaagtga aatccggttg ggccgacgac
    11761 gaacgcgcac tgcgcagtct cggttacgtt gacgatcttt aagagtcact ccgatggaag
    11821 gctggcagcg cgcatttgtc ctgcatagtc gcccgtggag cgaaaccagc ctgatgctgg
    11881 acgtcttcac ggaggaatcg ggtcgcgtgc gtctggttgc caaaggcgca cgctctaaac
    11941 gctctactct gaaaggtgca ttacagcctt tcacccctct cttgctacgt ttcggcgggc
    12001 gtggcgaagt caaaacgctg cgcagtgctg aagctgtctc gctggcgctg ccattaagcg
    12061 gtatcacgct ttacagcggt ctgtacatca acgaacttct ctcccgcgta ctggaatacg
    12121 agacgcgctt ctctgaactc tttttcgatt acttgcactg cattcagtct cttgcaggtg
    12181 ccactggtac gccagaaccc gcgctgcgcc gctttgaact ggcactgctc gggcatctgg
    12241 gctatggcgt caattttacc cattgtgcgg gtagcggcga gccggtagat gacaccatga
    12301 cgtatcgtta tcgcgaagaa aaagggttta tcgcaagcgt cgttatcgac aataaaacgt
    12361 tcaccggaag gcagttaaaa gcgttaaacg cgcgggaatt tcctgacgca gacacactac
    12421 gcgccgcgaa acgctttacc cgcatggcgc ttaagccgta tcttggcggt aaaccattaa
    12481 agagcaggga actgttccgg cagtttatgc ctaagcgaac ggtgaaaaca cattatgaat
    12541 gatgaggatt gtcatggctg aattactgtt aggcgtcaac attgaccata ttgcaacgct
    12601 gcgtaacgca cgcggtactg cttacccgga tccggtgcag gcggcgttta ttgctgagca
    12661 ggcgggagcg gacggcatta ccgtgcattt acgtgaagat cgccgccaca ttactgaccg
    12721 tgatgtgcgc atcctgcgtc agacgctgga tacccgcatg aatctggaga tggcggtgac
    12781 cgaagagatg ctggcgatcg ccgttgagac gaagccacat ttttgctgtc tggtaccgga
    12841 aaagcgtcag gaagtaacaa ccgaaggcgg cctggacgtc gcagggcagc gtgacaaaat
    12901 gcgcgatgcc tgcaaacgtc tggcagatgc cgggatccag gtttctctct ttatcgacgc
    12961 cgatgaagag cagatcaaag ctgcggcaga ggttggcgca ccgtttatcg agatccacac
    13021 cggttgctat gctgatgcca aaactgacgc cgaacaggcg caagagctgg cgcgtatcgc
    13081 caaagccgcg acctttgccg ctagtctcgg tctgaaagtt aacgctggtc acggtctgac
    13141 ctatcacaac gtgaaagcta ttgccgccat ccctgagatg catgaactga atatcggtca
    13201 tgccattatt ggtcgtgcag tgatgaccgg actgaaagat gcggtggcag aaatgaagcg
    13261 tctgatgctg gaagcgcgtg gctaatggca atattaggtt taggcacgga tatcgtagag
    13321 atcgctcgca tagaagcagt gatcgcccga tccggtgagc gcctggcacg ccgcgtatta
    13381 agcgataacg aatgggcaat ctggaaaacg caccaccagc cggtgcgttt tctggcgaag
    13441 cgttttgctg tgaaagaagc cgcagcaaaa gcgtttggca ccgggatccg caatggtctg
    13501 gcgtttaatc aatttgaagt attcaatgat gagctcggca aaccacggct acggctatgg
    13561 ggtgaggcat taaaactggc ggaaaagctc ggcgttgtaa atatgcatgt aacgctggca
    13621 gatgaacgcc actatgcttg tgccacggta attattgaaa gttaaaataa taccgttctc
    13681 tttaaaaagt aaaacccccg aactggtaag gaacgggggt tttacttttt aactttaatt
    13741 ttgtgcagca tgcaccctca acaacagcga ttatggatca gtgtacttat cttttacaag
    13801 caacttgcaa tctttagcat aaaaactcga gcctttacga agaaagcaat attgatggaa
    13861 agattaacgt gaccgccaat tcgtaagtac attaaaattg gcttcgttat tgaagatttt
    13921 gctgtgcttt acaccatgcc acagaattcc cccattgaaa cgagtggtgt cgtcaaagct
    13981 ctggtgtgga gtgcagcatg caccctcaat aactcgcacg ttcagttttg gggagatgta
    14041 agggctaatc tgaacggctg cattccttgt ttaaggaaaa acgaatgact gattgccgat
    14101 acctgattaa acgggtcatc aaaatcatca ttgctgtttt acagctgatc cttctgttct
    14161 tataacacaa ggaaacgtac ttaaggtgcg tccggtgaac caatcggacg cgcctttaat
    14221 aactataaat aagtgtcttg gcagatacta tataaattaa cttagtgaat gattatgcta
    14281 atgtcatcaa ttaaataaat ataatggcgt taaggcttcc cagtaatata attaatactc
    14341 tacttccaga gtagaatatt aaattttatc cgcgtggtgc atcagcacaa atttatccca
    14401 caactgttct tctgtctcga catgcgccgg atctttcaca atagtattgg ggatcgggca
    14461 caccttctgg caggttggtg tctcgtagtg ccctacgcat tcggtacact tatcgctgtt
    14521 aatctcgtag atatgatctc ccattgaaat cgcctcattc gggcattcgg gttcacacat
    14581 atcacaatta atacagcgtt tagtaattag taaagacatt tcaatggatt accgttaaat
    14641 cattttaaaa tcagtaagtt gtatcgagtt tgtatgcttt actgtcatta acttactgta
    14701 tgttgatcca gtgtatttaa ccttgataaa ctcagtccag caacacaaaa ccgcaacaca
    14761 ttgcattttg tcccgtagaa aagacttgta tgtgtgagct tgttttctgc gcctacgcag
    14821 ataaggattg agaatgccgc gcactgtaac acataatccg gatagcccca ataatgacga
    14881 tgttttagcc gcatctgaaa aatgggacgc ctgtaaaccc ccctatacca gcgcacacat
    14941 gaaaatctgt gttgctgccg ccaaaatcat cctcgctgct tccggcgtgg ctcgccgttc
    15001 caaatacgaa aaagagaact atctccgtat cgatttcagc aaagccggta aggttacatt
    15061 ttacgccgag tttccaaaaa agatgggcct caagggtaaa aagctcggcg agtggccgga
    15121 gctcgctatc cagctggcgc gcgaaaaagc gctaggtatg gctgacggtg gcctgcgggc
    15181 agagtccgta catgcagcgc tggaaatgta ccgggatgac ctcaaagcca aagtcgcccg
    15241 gcagaagctg agcccggaca gtttcacaac ctacggggtg cgtatcgacc ggattaaagc
    15301 cacgttcggc gagcgcgagg tgttcagcga cgtaacatac aatcggctgg tggaagtgct
    15361 ggacgagtgg atcgccactc gctcgaacaa taacgccctg gagttgtttg ccgagctccg
    15421 tcggttctgg aagttctgcg cacctactct ttgcaatggc cgcaatgttg ccgccagtct
    15481 gccagatgat tatgtttcct cccgcgtaca gaaacccacc cccacacggc tttttaccga
    15541 tattgaatca atcgcccgac tctggctcaa tgttgctgcc tgcacctctg tacaccagaa
    15601 gaatgctgtt cgcttcatga tcatcactgg tgttcgtccg attaatgtcc ataacctgcg
    15661 ctgggattac gttcacgagg aggctggtga aattgtttat ccggaagggg ttatcggcat
    15721 gcgaggggct atgaaaacac aaaaggcttt ccgcctgccg ataacgcctg agatccggcg
    15781 gattatcgac gagcagaaag cctggcgtga ttcagttcct gagtgcaaca gggattatgt
    15841 atttttgcag ccacgtgatc caatgcagcc attttcaaaa cgatcactgg ataagctggt
    15901 gaaaacatac agcccggacg gggctgtaaa aggaataaaa catgatggga ctgttaaagg
    15961 gaaagacggt gcatttaata cgatgtgccg taaattcctt aagagcaatg ttattgcctt
    16021 gatgaaggaa agaggctatt cccgctcaga ccgaagggaa atcagcctcc tttgccttca
    16081 ccactccagc aagtcagatg acccgatggc agaacattac gacttttctg atgagatttt
    16141 acaggaagag attgcgttga agcgcgaagc tttcgaggct cacgagcgga gcatacttgc
    16201 gcaggtggca ttgctacggc ggcgaggtta atactggctg cgacattttt gaataaaagc
    16261 gtcgacattt cggcgctcat aacgaactac ttttgcactg aaacgaattg gtgccaggat
    16321 agcccgatga cgatgcttaa tattccactc acatagcgtt ttctgtgtaa tacctaactt
    16381 ttggcatact tcatccgggg tgagtaaatc gtcgggtttc tcgctcatgc tatacctctc
    16441 tttttcatgg catcgagcag gatgtcctgc actgttcgtt ttgagttgcg ccgctccatc
    16501 accatttcgt ccatagtgtc ggcagcaata atgtggtgaa taaataccgg acgattgtgt
    16561 ccggcctgta tctgtctggt tgggccgata cgttcaataa tttgctgata ctgctccagg
    16621 tcccaccaat gtgagaaaaa taccagtatg tttccgccgt cctgcatgtt cagaccgtgg
    16681 cccgcgctgg ctgggtgtgc aaagagaaca ggaatctttc cggaattcca gtcacgtagt
    16741 gtctgtggat cctggtcgag gtgacgaccg cgaggaaatg ctttaagcaa gcgttcaaga
    16801 tcgtgtttcc agtgataagc aaccagcaca ggtgcgccag ctgcttcggt cagtatgctg
    16861 tccagcgcct gtagtttggt gtcatgcagt tctgaccaac ttccggtgtc atctgtgtat
    16921 actgcgccgc tggcgatttg cagacacttc agtgtctttg ccgcggcgtt cggtgcttct
    16981 atgccttcgc cattcagctc gaggaacatt tccttttcca tttcacgata ctgctgacgg
    17041 gccttcggcg gcatatccac gcggattacg ttatggatgg ggtctttgat atcgaaccag
    17101 tcggtcgcat ccagcgagag ggtcacatcg gctaacgctc gctgtatttc accctgtgag
    17161 tgagcaaaag gctccagttt ggtccagctc tgccccggaa actgtatcga tttgaaccag
    17221 cgtgaggtaa acgcgccgta agtgcgcccg agacgttgcc ccaggtccac aaaccacgat
    17281 tgtccccaca aatctaccag gccgttcggt gctggcgtac cggtgagatt tatccagcgc
    17341 cggacatact tgtgcgccac tttgcccagc gccgccgcgc gcttaccacc acctcgcagc
    17401 cggaaggatt ttagccgggt gctttcatcc ggaatgacag taccgaacgg ccatcgttct
    17461 cccaattctt caaccagcca gacaaggtta tcgtagttga tggtgaacac gcttgcgttg
    17521 ctgttcgcca gcgccgtaga gcgcgctttg acattaccaa caatcggctg tacctcgata
    17581 ttgcgcagat gcttccattt cagcgcttca tccggccatg tgcttgctgc aacgcgtagc
    17641 ggcgcgagga ccagtgcggg gcgtgtttct gcccctgcca taaagagatc ttccagcgta
    17701 gtgagcgttg ccacggtttt acccatcccc atccctgccc agatgttgcc gcgcagggtc
    17761 tcgatttgat gattgatgat gagatcttgg tagggacgtg gtttaaaagt tatcatgaag
    17821 aaaatccctg attagaaagg tgctcttgaa catcatgaat gtaaaagatt tatcaattac
    17881 aacggttctc ttggccgccc tttattttat ggctttttct ttttacaaag gatacagctc
    17941 attttatgga ttccctgtaa gttttatttc tattgggatt gaggaaatag ttaagttttc
    18001 tgttattgca ttaggacttt tgtgcacctt agtcgctttg ctgcatattg atactgaaga
    18061 gaagaatatt ccatgggggg tgtatttatt tttcttcatt ttggcctctt tgctttcata
    18121 ctggacaatg tatttatatg gcggtagctc atacctttat gaaaaatcca taagggatgt
    18181 ggctacccaa gcaatactat tgggatttat tagccctatc agtgtcaggg cagtatctgt
    18241 ttttattagg aatgaattta gaataaaaaa caaaactcat ggggttatgt tttttatatc
    18301 catagcaatg cttccttcag tccttgggtg ggcatgggca tacttatcag atgagcctct
    18361 tttttattct aaaaaatatg atgcttatat tatcgaaagc tataacggaa aatttgtctt
    18421 ggggaactgt aaaaaaaata gctctgaatt tatgagtgtc gaaggaattg aggggaaatt
    18481 aattcctgta agcataaaag aaacacaaca actaaaagta tgttttttaa aagcttctaa
    18541 gctcaagaaa aatcgctcag aatgattgtc tcacaatatc ccctctaaat ttttgctatc
    18601 cagtactacc acggtaaagc ccagctttcg cagacgttca tgctcgcgca actggtcagg
    18661 ccgtggtggt ttgccggggg atttacattc aacgaaaacg atacgaccac cgggtagcag
    18721 gacaatgcga tccggtaccg agcggtgacc gggagataca aacttaaagg ccactccgcc
    18781 agcttttttc acttcagcga cgaggtgctt ttcgataagg ctttcacgtt cataggccat
    18841 ctgaatccca ctcttcaaaa agcacattca attccagttt ctctgccaac gcgttttctg
    18901 cacgtgcgcc ggcagagtac tcccactctt tgagcatata aatcgtatcg gcacagcgaa
    18961 gcattgcgag gcagatgtcc atgtactgag cctgtgtcaa accatcgggt aaagttgcgg
    19021 ggttaagcac aacatatccc ttgtccgtta gcctctttgc tgcggaatga aaagccgggc
    19081 ggttaaattg ctcgtaaccg ctcatcggcc cggcaacgta aacgatcatt cttcgaccac
    19141 cttacgcttt tcacgcatat tctgcatcag gcaaaaatca gatctgcgtt cactccattc
    19201 ctgattcaat tcgtgatgtg attcgcggtt ggcttttgcc cagaccttcg ctgcccggtc
    19261 atattcgccg gattgttcaa gtcgcaaagc ctcccttgcg gccctgtaat aaagtggatt
    19321 gtcccggtat ttaaatgaca taggagttaa tccttacggt agtggtacgc ctcaaaaccg
    19381 ccagcgttca gtgggatatc gggtgcccat tcggggttag tggagagaag cgcggaaagc
    19441 gctttatcgt tgaaatcttc tgtgtcaggt gcttcggtga tcacctcgtc gtgtaccgtc
    19501 aacacaatgc tgtaaccggc atcttcgata agcggcatgt ttccggccag aacgtcgcgg
    19561 gcggctgcct gggtgacgtt ctccaccagc tttccgccgt aggttttgag tcgttgccat
    19621 ttacgcgaat aagagttaac acccatgtag gtgatattcc ctttttcgat aaccggagac
    19681 gggtagcata cagcgcgtcc ggatggtagc tgtatgcgca gccacgcgcc atcacgtcgg
    19741 attttaagat agccgcaata caatgttttt tgcggtgtgg cgattgctgt gcggacggtg
    19801 cgctccagtt cgtaccagaa atcgcaggtc gccgggtgcg ccctgcgcca gagacgttta
    19861 agtgagtcgc aggcgataaa tacccgttca gaaagcccgt aggttgactt gcgtttaacc
    19921 gattcgtcgt accagctttt cgcctcgcgg ataacatcgc ggggaatgtt tggtagtgcg
    19981 gcgttcgcca gctcgtcgag atcgagaccg taaaccagtg caaaagtgat gaaggccgac
    20041 acaccaccac cataacccag accgagttcc atgactttac cgatctgacg catgtgttta
    20101 tcaacatcat ctggtgcaat atcgaaagct tttgcatacg ccagtttata taagtccgga
    20161 cccgttccgg cgtcgtactc tctgaatgca ttcagtttcc attcttctcc cgccagccat
    20221 gccagcatac ggccttcaat gttcgacaag tcacttacca ccagcttttt gcctgttggc
    20281 gcgataatgc agccacgtaa cgctgaactg gttagttcca tgatattgtc aaacagcagg
    20341 tctgcacatc cggctttcag tgcttcgatg ccttcgtcta tttgttcctg ttttagtgaa
    20401 gggcggggaa ggttctgggg ctggaatagc cgtccggccc aacgaccggt acgtgacgcc
    20461 ccgcagaact gtagcgtacc gcgtaagcgc ccgtcgtggc ttacgccttt catcagtgcc
    20521 ttgtatttac tggtgctggt agtactggcc tgcaggcgga tagccagcag ttctttcacg
    20581 gcagatggta aatcggggtc ggcaatacga cgttccagag tactgcgttg catgtctggt
    20641 agctccacac cgtaggattc aacaatgtgc ttaatcaacg cgtctcgttg tgtggctgcc
    20701 tgcacttcgc catcagtcat ttcctgtgta cgctttgcta ggcgcttttg ttcctggtct
    20761 accgcttcga tcgcagcgcg tgcgagttgc atgtccatgc agacgccccg gtcattgatc
    20821 tgctgatcac gatgccagag cgccagttct gtcccctgat aattccactt cggcagacgt
    20881 ttatagactt cgcgcattgc ctcgatatcc agtccggcgt aagcaacaaa gcgccgccat
    20941 tcttccgggt gggttttact ggtggcccga cgcagtttgc tgtttttcgg gcgtggctta
    21001 cagaacagct ggatcagcgc tttaccttct ttgtccttcg ctttgtcttg cgggacgcct
    21061 agtacttcgc agagttcccc cagagacccc gggagaccgt gcgccagcgc ctgcaccatc
    21121 gtgtcgcgcc aacgttcgac tggcggtgcc agtcgcggca ttgtataacg cagaacggtg
    21181 cggtcgaagt gagagttatg gaaataaagc agggtttcag ggtctgcgat tgcttcgtat
    21241 aagccgtgtg gaataccacc accggcagtg atatcccaca cgtttactgg cccgtcgttg
    21301 atagcccatg cgaaaagcat cacttcaacg ccttcggcat acgcatgggt accgttgttg
    21361 atcggaatgt cacaataggt ttccaggtcg ccccatagta tattggacat aaaatatcct
    21421 catatggtat atggttaaat gctagatatc atgaattaaa ggagacaact aatgactgtg
    21481 ggtaataaga gacagcctaa tggattacct gaatattatt gtcaccgtgg taacgacata
    21541 aaagatgcga tagttcggga ttttattatt cagcctgttg cacacctgaa acttattact
    21601 ggacaaacta aaaccagtta ttccggttat ccgttaaccg atcagtacta ttgtttttca
    21661 tttaaaaaac gctctgatga aaattgtaca ggggcttttt tctgtggtag agatgttgca
    21721 aaggatttca ttcggcgtat cggttgtaca gagctgccgt tatttaatcc tatgaaatcg
    21781 gataccagtg cagcaataga acgttccggt tctggctcag gtagttctgg tattactgtc
    21841 aagccaaatg ctgacaaaaa gctatttata gacgcagctc gtctgttaat gacgctgtgg
    21901 gattcaacaa gtaagggaca aaccccactg gcgtcggttc ttaatgaagc aatttctggt
    21961 ggtatagata aacctccaga aattagacat gtagtggcgg ttaatacaat gttgggtaac
    22021 acaaaaaata ctttaactgg tttgctggat aaagcaagag cgaagtatcc tgatgtgtta
    22081 ttcagagatt ttgatttcac gcctcttaac gagattgtaa aacgttcaaa atcatataat
    22141 aagcatcccc cttgttattg agtatcagac actgtattgg taggaaggcc tatcatttca
    22201 tttaatgtct ctcgatgtac tgctggtgct aaagggactg gcggtgcgtc tgttggaagc
    22261 agttctttag cttcgggcca tacatccagt aatcgtttta ctgtccgaac tttatttaga
    22321 gccgcgctga cgttctgtat gatttcggtt tcatcacttt taatttgtgt gtagagagca
    22381 tcaaagctat aaaactccgt taccagagga tcttcgccaa gtagagtaaa ttctgcaggt
    22441 gctattttat taatatgctc gggggctcct gattcataac ctctgtagtt gccattgaaa
    22501 taaatgtttg ctctggctcc agccagattg agatatatgc agcaatcttt tcgaattaaa
    22561 gaactattgg tttttaatga atcagggatt tttgttacca tggattcaat cttttttata
    22621 ttttttaata tttcagcttc tttctctacg cccccgatag ctgcaagccg aacacgttcg
    22681 gcccattcaa cacgagcagc tcgtagtgct gatttacgtt tggggatacc cgctttctca
    22741 agtgcgttat tgatgatttg agttttaagg tgattagtta atacgggctg tgacattatt
    22801 ctttccctca tgagataccc cagcacgtag ctgggggatg aatattttta aattagtgct
    22861 tcagcatcag caccttcgct gatatcgtcg aaatcgtcag cgcttgccac tccgccgcca
    22921 gcgaatgcat cgccgtctcg caggaactgg actcctccga gtgaggcatt aatgcgttta
    22981 ccgaaattat tgtcctgtgc ccagatatcg ataacggcgt ttacatagca ccctgcatag
    23041 ggacgtccat cagcctgaat tagtggcgaa cgatcgcgat caagaacagc tgggcgcgct
    23101 ttgttagcag cgttcaggaa gaaattgccg ggaaagcctt catactctgc tttttcatca
    23161 ccatcatgca ggcacagatt gagttttttc tccagctggt tataaatgga ctcccacttc
    23221 tccccccatt tttccttcgc tacctgcttc atagctttac ggatttcttc cagttgtggg
    23281 tgtttgggtg acattaaaaa tactgcggag aaacgtggat cgccttcgcc gtttactgtt
    23341 ttagcttcaa acagagacgg gaaggccaga cgaacattgt tcagcttcag tttcatgggt
    23401 atttccttaa atcagatgag gtctgcggtt agcgtatcgt cggatacgtc gtcgaaatca
    23461 tttacagggt tgatattgag tgcggggcgt gggtctgact cgggaacgac ggtgggttta
    23521 ccatcagctc gtgttatcag tgcctcgact tttgaccaac ggcgcggact ggcctttttg
    23581 ataagttttt cggcttttgt gggactaata agtttaaagt cgaatacttc ttcagttttg
    23641 tacctgaact ggtccttcag aagtgcgcga gctgcctctt catcaatcca ggcccggtta
    23701 ccttgttttc ctgttaccag tttaaacccc ggtaccggat gtccggcatt gagttcattg
    23761 tgaacccggt cccgtactgc ctttagccag gattcaataa agtcggcctg gctatagatc
    23821 tccgcaagct gctcaatggt taacagaggt acacgtgcac tggcattggt gattatttcg
    23881 ctgacaggct ttgtcagatc ttcaaaatcg ctggccgctg tttgtaaatg ctgcattttc
    23941 tgggcagtgc aaatagcttt tgctttacag aagcggcact gtttttctcc aggtatgaag
    24001 ttttccagcg gtagtgtctc aatgccttcg cattcagcaa tattgagaac aaggatcgca
    24061 ctggttgcgg cctcctgtgc ccgttcaccg aaagactgaa gttcctgtac ggttaacgac
    24121 cattctgaaa cgtggttgag ccttggctgg tgaataaata atcttacagt ctcaaagtca
    24181 tacagcatgc tgaattgttc gagcgcaccc agagcataca gttgtagttg ctcattttgt
    24241 tctgcatcaa tgcggacgcc tttgccatat ttcaggtcgt ggatttgtaa ttcgttaccc
    24301 gcgatgatta tgccgtcggc agttccgaaa gattcttcca cacccgttat atgtgagaaa
    24361 tcaacacgtt gttcaaccaa tagttcatta ttctgtgcaa gagtccagac cgtatcaaca
    24421 taccggccaa cggcttcgac catttcatca tccacctgtg ggccagatgt atcatcagga
    24481 ttttcgcgaa gggggtatga gccgagaaac atagaaacat tgcatccggc gtagtgttcc
    24541 ggatggcttt gcctgtttcg tagaactttt tcagcaagcg cgtgcgctgc agtgccctcg
    24601 attgcaaaag ttgtttcttt atccggttgt gtagcctcca gcgccagact tcctgggcag
    24661 cgcatccatc gatgcgctga tgatggagaa agttgtgcat gaacgtctgg catgattaac
    24721 cctccagtgc tttttcagcc agggtgatta cttcagcgag attttcatcc gttacttcac
    24781 caagtttcct ggctccctgt ttttccagaa ttgcaatagc ttctgcccgg taaccccctt
    24841 ttgctaactg gaggatcaac ccttcagctt gtttgcgtag tgccgcgaaa tcaattgtat
    24901 ggtcatcttt ggcgtcatta ttctggctgg aatttgctgc gtctctgcgt gcaaattctt
    24961 cctgcagctg aaggtactca acacggttga tctcgatatg gccttttttc agcatctcgt
    25021 tcaacttgcg taaggtgtgg agttcactgg ctgctgtgcc ggatacattt ttgacgtaaa
    25081 acggccccgt gcgttctcca tctttgttac tggccttttt cggcttaact tcatcacgcc
    25141 catccgcagg tgcatcaagt agctgctcgg caaaagcacg ccgctcgccg atggttggca
    25201 ggtcgtccca gaacttaagg atgttacggg acaggtccag gagagctggt ttaagcagcg
    25261 ccctggctcg tttgacgccc tgcaatgcgc tgtcgagagc atcaatctga actactcgtt
    25321 tatcgccttc agcatcacgg taggcaacag cacgttgcag catgtcttct gtgatagggg
    25381 tggctaccgg gtagaaacca gccagtgcga taacgtcgct gaactccaga tcatccagtg
    25441 tcattgccgc tgacatattt tcagtttcag ttgctgtatc ccgacattcc tgcactcgtg
    25501 aaatcgtgtc aggatgcata acaatacctg atgccattgt gcggataaga cgttcaagca
    25561 gcgcattatg ttgtgccaga agttgattat taagttcgag actggtttct aaactcatac
    25621 tgtggtcctc gctacaagga gaatgaaagt gatgatcaga ccgagcgcag tggcaacggc
    25681 cagaccggtc atcaaatcga agtttttacg gcgataacgg agaacatcgc gccccgtcag
    25741 tcgatagagg tgttcaggtt tcatcggttg tattcctttt ttcatatcgg ggagcgcgct
    25801 gttgcgagtg cgctttcaga cataaaaaag cccgtcactt gcggcgggca aagactacac
    25861 acagcaatta catggatgat tcagttggta ttgtgcgtac gtggtaccac aaggttgaag
    25921 cggacacgcc agtgcgttgc aatggtatgc aggaaagcac cacatccagg gcagtgctca
    25981 taacattcgt tgatttggta tcgttttggc tttggtgtct gaagctgtgt ttggcaagca
    26041 gggcattcac ctttgagcgg tttatacgtt ttcacgccgt ttcctcattc agttcacttt
    26101 ggtggttcgg cagcttcgac ttgtatcagt agacagtgct tcgccgcacc ccaaagggaa
    26161 cttactgacc cgtattgccg acatcctgtc ccgccacggt cccgacgcat ggtttagagt
    26221 cgcgccgttc gacttgtggc taaagataca atttgagttg tattgtgtaa accacaaatg
    26281 tggtattttg tggggtgtga atactacttt attgatattt aagtgaattt aatttgtaag
    26341 actttacaaa tggggtaagg gaggctgtta cgcctcccga tatttacggt gggaatacta
    26401 acgtttacgg cggtaaatac ggtgttcgat cattacacct atgatctgta agttcatatc
    26461 tgcactacga aaaatagggt aatcaggatt tagggggatt aattcaaagt cgtcgatgcc
    26521 gattcctaat ggacggtatt ttttaaatgt agcttcgtga ccgtcgtttt tggcgacaac
    26581 aaactcccct ggtgcaggac atagatcagg atcgatgata acaatgtctc cttctttaaa
    26641 ctctggttgc atgctgtcgc catcgatacg taatgcgaag catgtttctg gaatgtcaga
    26701 gtctgccaaa atatattcaa attcccctgt cagatctgtt atgtctcttg cttctgtaag
    26761 ctctcctgcc tgaacgtaac ttaatatagg tatgcgtctg gtacttatct ccgcgagagg
    26821 cataatattt ttaccgttca gtagccagtc tgggctacat ttcaaggcct tagccagatc
    26881 cagaagattg cgtggctttc tggttcgtcc actttcgatg gattctatgg attgttggct
    26941 aacccctgca gaattcgcta cttccacttg agtcattcct aattccaaac gacgtgcttt
    27001 aaaacgggct gcgagagaca tgggattagt ccttctgggt tgtattaatg tgtatctcca
    27061 cattaaaaac aatttttgtt gtatttgaca aacctcgttt gttgttgcta aataccacta
    27121 aaattgtatg aggtgaaagc aatgacctta gcgacccgag taaaagaacg acgtaaagaa
    27181 ctcaaaatga cgcaagttac gttggctgag ctcacaggag tgagccagca ggcaataaac
    27241 agaattgaaa gtggtgttat tgctcggcca cgttatcttc ttgaaatgtc tatagcattg
    27301 gattgcgacc ctaattggct gttgtatggc tcacaaaacg ataaaaaggc ttaacccatg
    27361 tccgatagca aaccatgggg agctacgcct gatgagtggt ttcatttcga cctggtattg
    27421 gggagaactg atcatcttct gccagttgta tgtaaccccg gtgcgaccat atcccctgat
    27481 agtaaactga aagcgttggg taagacgccg agtcgctata accgggaccg ccaggtcacc
    27541 ggtattgctc aatggaccgg gcatgttgtt actgagcatg attttgcccg ctggtcgaat
    27601 gaaccggatt atggcatctg cgtgcgtaca ggccatggct ggctggcgct ggactgtgat
    27661 agcgaagatg aagacattca ggcagatatt cgtaaaacgc ttgtgcaact tctgggtgag
    27721 ttgccgccac gacgctggcg agcaaacagt aataagtgtc tgtatctgct ggccgttgat
    27781 ggtgatttcc gtaagcgtat ccatcgcctg gcgggggata tgggcattat cgagttgctg
    27841 gcgaacgggc agcagttcgt tgcctgcggt acgcacagca gcggcgcgcg tattgaatgg
    27901 gacggcggtt tgccggatga acctccggct attactggtg agcagcttga aacgctgtgg
    27961 cagcgcctgg ctgaacaact ccctgtgtcg gtaaccaccg aagcgggcaa cacgaagatg
    28021 cgcgaccgat cagcattcac gcccggcgcg acggatgata cagctgaata tcttgatgcc
    28081 aatggctgga cgctgctgga tggtgcaaat ggtgaacgat atatccgctg tccgtttgaa
    28141 gacggccaca gtagcggggg cgatccaaca agcacagttt attttcctgc gggaaccgcg
    28201 ggctttgagc aggggcattt taaatgcctg catgccagtt gtgcgcatcg tgatgacgga
    28261 gatttcctta atgccatcgg gatccgcaac gacgatttcg aagatctgac cagcaccgaa
    28321 gtggcggaac ctttaccgct gcctgctttc gagcgtgata aatgggggcg tatcgaggca
    28381 accatcagca acgcagccaa agcagtagta cgccctgatt ttgtggacat cgatattcgc
    28441 tttgacctgt tccgcgacga aatcatgttt gccccggcag gatccggaca atggcgggca
    28501 ttcaccgatg cggattatgc gcgcctgcgc atcacgatgg aaaagcgggg atttaaacct
    28561 gttggccgtg aacttattcg cgatgtggtg ttacttgcag ccgatgaaca accattcgat
    28621 tcagcgatcg cctggctgaa cggactggag tgggatgggg tgccgcgcat cgaatgtttc
    28681 taccatacgc acttcggtac cgccgacacg ccttataccc gtgcggtgtc tatgtacatg
    28741 tggaccgcgt tggcggggcg agtactggag ccaggcatca aagcggatat ggtgccgatc
    28801 ctcgttggtc cgcagggctg cggtaagtct tccggagtgg aggcactgag ccctgatcct
    28861 gcgtttttta ctgaaatctc ttttgccgaa aaagacgatg atctcgctcg aaaaatgcgt
    28921 ggtcggctgg tggcagagat tggtgaactg cgcggactta ataccaaaga gctggagtca
    28981 atcaaagcgt ttgtgacgcg tactcacgaa aactggatcc cgaaataccg ggagttcgcc
    29041 acccagtttc ctcgtcgcct ggtgttcgtt ggtaccacca atgaggacga attccttgcg
    29101 gacaagactg gtaaccgtcg ctggctcccc gtggaagtgt cgaaagtcga cgtgaaagcg
    29161 ataaaaagag atctcctttt actttgggct gaggctcgtg aggtgtttca gcgtctgggg
    29221 ggtatccagt tccgtgaggc tgaacaactg gcagcgagtg tccatgaaca gtacaccatc
    29281 aaggatgctt ggcttgaaac ggtagagaaa tggctcgaca cgccagacct gatgactaat
    29341 gaacttccgc gaaattgcga atttttacgc gcaagtgatg ttttgcgtga tgcgattggg
    29401 ctaaatcctg accgcatcgg aaaacgcgaa gaaatgcgaa ttagtaatgt tttgcaaaat
    29461 tgcgggtata agcgtgccca aaggcgaatt ggggggaaaa tgactcgaat ttttgaggcg
    29521 gtgtcccaac ctgtaccagc ctcaaaatag aggttggtac attttaacta attgaattta
    29581 aaggtcagta ccaactgtac catctgtacc aacctaatta ctaagaaccc catatatatg
    29641 tataagtcgc ttggggaaaa ggtttgaaaa gagctggtac aggtgggtac aggttggtac
    29701 aggccgaaca aggaattttt tgcacataac agcgtgtaat aagcgaatcg gaactgcgtt
    29761 atccacaccc acggataaac agacgtggtt ctcagaaaat tttttcgtag caaaacgtag
    29821 aggtcagagc tatgcgtaat attcaacagg ttttagagcg ctggggtggc tgggcagcga
    29881 gtgaaggtgg tagcgtctac tttcctcctg ttgcagccgg gtttaagaat ctgctacccg
    29941 cgacgcagtc tggaaggctg aaatgcagtg acaatgacgg tcttatcatc aactccgcta
    30001 tgagctgcct gaagaaaaaa gatccgtatc tgtgcacgct ccttgagtgg cattacgtcc
    30061 aggccatgcc cgtgcgggcg atgggtgaga agctcggcgt atctcacacc cacgttctga
    30121 agaggcttca ggcggcagag ggatttattg acggttgctt agccatgctg gatgtggtgc
    30181 tcgaaatgga tcagtcggtt cagtcaaagc ctcaggctat caggactttg cgtaggagct
    30241 gctcggcggc ataatatcca gcaatcaatc acgtaaggga accagatggc tctgatcagc
    30301 gttcgcaaca gatttgaaag cttcatggaa cagaggtacc cagacctgtc gttgcaggtc
    30361 aaaggtaata ttagcgcatc aatgaaggct caacttggca tcaggattga acgagaattg
    30421 tacagtgaag atgttactta ctgtgattca gcagttcagt tgatgtggac actctttcag
    30481 gcaggtgttc tggccgaacg aagagctacc agcgttacgc ttcctgcgct gaaggcaaag
    30541 ccggatagct tctacgatgc gggttataac gaaggtattc aggactgtcg taaacatctg
    30601 acggcatcag gcatcaaggt aagataaaaa atagttgtgg aattccaaaa agccgattag
    30661 cctgatatcc gttgaaaaca gttcatcacg aagaggcttc cgcaaggggg ccttttttat
    30721 tgccccattc tggggaaaag ttaataaaac agggctttcg ctgcgaaaaa acgctatgca
    30781 gtttttgccc ttttttatgc accttttatt cactcgaatt tcgtcattct ggaccactta
    30841 agttgattaa ataggccttt catcgcaaat ctattgcgag cggtgatcgt gtggttccta
    30901 taacgtacat tatgttaaat aacttccttt tttaacaaat ttaacaaggt tcgctatggc
    30961 gaactttttt tgtattcagg gcccaccgaa ggacggctca taacccaatc ctacgggcgt
    31021 atacgcaggg cccgcctttc aacaacaccc cgtaatggcg gaggtgggaa gtatgaaaat
    31081 gcacaatgct cctcattcct ggcctgactt actggaactc ttacaaagtt ggtggcgtgg
    31141 agatacgccg ttgggcgcag tggttatgtc aattgttatg gctggcttgc gcattgccta
    31201 ttttggcggt ggcggcggct ggaaacgaaa aacgcttgag attttgctct gtggtgctct
    31261 gacgctgacc tttgcatccg ctcttgagta tgttggatgg cctaaatctc tttctgttgc
    31321 cattggtggt ggtgttgggc tgatcggtgt cgatgctatt cgtggggctg caatgcgagt
    31381 aatcggtaac aagtttggtg gctctaagga gtaattcatg cagacactaa attcccaacg
    31441 taaagctttc ctggatatgg tggcatggtc agaaggaacg gataacgggc gacaaccgac
    31501 acgtaatcac ggttatgacg ttatcgtcgg aggtgagttg ttcactgatt actccgatca
    31561 ccctcgcaaa cttgtcacgc taaacccgaa gcttaaatca acagctgcag gccggtatca
    31621 acttctttca cgctggtggg atgcctaccg caagcagctt ggcctgaaag atttttcgcc
    31681 agaaagtcag gacgctgtgg cgctgcagca gattaaagag cgtggcgctt taccgatgat
    31741 tgaccgtggc gatattcgtc aggcaatcga ccgttgcagc aatatctggg cgtcgttacc
    31801 tggtgcaggt tacggtcagt atgaacataa aatcggtgac ctgatttcca ggtttaaaga
    31861 tgctggtggg gtggtaaatg aagctgactt ataagattgt catcgcggca tttttcttct
    31921 ctgcctttgg ggcgctcgtc tggtctgcaa accattacca cagcaagtat cgggcagaaa
    31981 agttgcgggc tgataaagcg gaaggtgaag ctgaatatca agggaaagtg atagctaatc
    32041 aggcattaaa cttcaatcgc tttaaccaga tagcagaaaa cgcaagccga ttaaattctc
    32101 tggtcgacat cggtcacgag aagacagtca tcaaataccg tgaggttctg ctccgtgaaa
    32161 agaactgtga tttccctgtt cctgttgata ttgctgtcgg ggtgctcaac tacgcgaacc
    32221 gtttacgtgc cagcgcattg cacgccgatt ccggggacat tgactcagcc ggtgatcgtg
    32281 ccactaccac cagaacgttg acatattgcc aggctgttct gtggattaac ccactgttgg
    32341 cagccatcga gaaggcgaat aaccagttgg ctggtgtccg acaagtagaa cagtcccggt
    32401 aatagcatta cagaagctct tccaggaggg gcttcgataa tgacctgata actggaaaat
    32461 aaaatgacta agaagctgaa agcaaaacac gaggtgtttt gtcgcgagtt tcttgtcgat
    32521 ctgaatgcta cacaagcagc tattcgcgca ggctacgcct ccaggcgagc acatgttacg
    32581 ggggctgaac tatacggtaa acctgagata cgcgcccgca tcaacgagct aaagcaggag
    32641 cgtattgatc aactggacat tgatgcgaat tatgtgctga tgcgactggt tgagatcgac
    32701 aggctcgatg tggctgacat cctggaggac gatttaagta ttaagcctct gtctgcgtgg
    32761 ccggaatcgt ggcgtcggta cctgagtgga tttaacctcg ctgaaatgtt tgaggggcga
    32821 ggagatgaca gagaaatggt cgggatcctt aaaaagatta agtggcctga taaggttaaa
    32881 aaccttgagt tgcttgggcg tcatgtttct gttcaggcgt ttaaagacaa cgtcaaaaat
    32941 gaagtgactg gcgctgatgg aggaccagtc agaacagaaa ttaccaactt aacgccggag
    33001 caggctgcag aggcgtatag aaaaatgatg ggctaagtat gccgttacca ttccccttcg
    33061 attttaaaca tcctgattac cagatggttt ttgaatggcg gatggaacgc ctacagcgca
    33121 ttcgccagaa tcctgaaata ttgcctgcac taaaacagtt ttaccgaacc aatccggctc
    33181 agttcatcat cgactggggc atgacaacgg acccgcgtaa tattgattat ggcctgccgg
    33241 tgaccattcc gtttttactc ttccctaagc aggaggagtg gatccactgg attatggaac
    33301 gctggagcaa tcgggagaat ggtattaccg aaaaatcccg tgaaatggga ctcagttgga
    33361 ccgcgatcgg actggcatgt tcgctttgtc tcttcaacaa agaaatggtt atcggtttcg
    33421 gctcccgtaa agaggaatac gtcgacagca ccggtgaccc gaaagcattg ttctggaagg
    33481 cgcgcaagtt cgtggaaaca ctgcctgtag agtttcgcgg ttcgtggagc gagaagaagc
    33541 acgcgccata tatgcgtgtt gagtttcctg aaactggtgc cgttatcaaa ggcgaggctg
    33601 gcgataatat tggtcgtggt gaccgtacca cgctttatct ggttgatgag gctgcattcc
    33661 ttcagcgtcc tctgctgatt gatgcggcgt tgtcacaaac gacgcgttgc cgtatcgacc
    33721 tgagttcagt taacggcatg gcgaacccgt tcgctcagaa gcgtcatggc gggaagatac
    33781 cggtattcac attccactgg cgggatgatc ctcgcaagga tgaagagtgg tatcgcaggg
    33841 aatgcgagaa aatcgataat ccggtggtgg tggcacagga acttgatctg aactacagcg
    33901 catcagcgga aggcgtcctg attccatccg aatgggtaca ggctgccgtt gatgcgcata
    33961 tcaaactggg tatccagcca acaggcaaac gacttggcgc gatggatgtc gccgacgaag
    34021 gcagggacaa aaatgccttt tccacccgtc atggcttcct cctggaaaat gtgcgggaat
    34081 ggtccggtgt gggcagcgac atttatcagt ccgtcgagaa ggttttcggc ttttgcgaac
    34141 aggataacct cgaagagttt cgctttgacg aggacgggct gggcgctggc gttcgcggcg
    34201 atgcacgcgc tatcaacgaa ctgcgtaacg ctgtgcgtcg accgtcaata ctcgccacac
    34261 cgtttcgagg tagtggcgcg gtatttgatc cggatgatga agctgttcgc ggggacaacg
    34321 ggcaagccgc acgtctgaac aaggacttct tcgctaacgc caaagcccag agctggtggc
    34381 ggttacgtaa actttttcag aatacctggc gcgccgtggt tgaaggtatg gcttacaacc
    34441 cggacgaaat catctcaatc agcagtagca tggcactcaa agataaactc atcatcgagc
    34501 tttcgcagcc gacctattcc attaatggtg tgggaaaaat cgttattgat aaacagcctg
    34561 atggaacccg atcgccaaac cttgccgact cggtgatgat caactatgcc ccaatgaatt
    34621 cagccctgaa catctgggag ctgctaggga gacaggcctg atggcacgaa acaaacaagc
    34681 cctgcggcga actgcgcagg ccacagctga tggttatgag aattttattg cccgcgtagg
    34741 gatgcagaca cctaaccagc actcagcatc cacctaccgg gctaatttca ccagtcgtaa
    34801 ccgcatgctg gtggaatggt cctatcgttc atcctggatc atcggcgaag cagtcgatgc
    34861 tatcccggat gatatgaccc gcaaaggcat tcgcatcact tcggaaattg atgcaaaaga
    34921 tcgtggcatt ctcgaatcac aactggatga gttgcaaatc tgggatgcgc tgaatgacgt
    34981 gctgaaatgg tcgcgcctct acggcggcgc ggtgggtttc atcatgattg aggggcaggc
    35041 accaatgacc ccgctgcgac ccgaaaccat cggtaagggc aagtttaagg ggattctccc
    35101 gctcgaccgc tggatgattg ccccggtact gacccgccgc attaaagata tggggccgga
    35161 cctgggtaaa cctgagtttt atgatgtggt gaccacagca acgggaattc ctgcctggcg
    35221 cattcatcac agtcgcctga ttcgctttga tggcgtcacg ctgccatttc agcagaagat
    35281 gaccgagaac gaatggggaa tgtcggttgt agagcgtatc tgggatcgtc ttaccgcgtt
    35341 cgacagcgct actgtcggtg cggcgcagct ggtctacaag gcgcatttgc gtacctacag
    35401 cgtggagaag ctacgcgagc ttatcgcact tggtggtcct gcgtatgaag cgttgctgaa
    35461 gaatattgac ctgattcgac agttccagag caatgaaggt atgacgctca tggactcgcg
    35521 ggataagttt gaaacccatc agtacagctt cagtggtctg gatgacatcc tttcacagtt
    35581 tgcagaacag attagtggcg ctgttggtat cccactggtg cggttgttcg gacagtcccc
    35641 gaagggattt tctactggcg atgcagacct tgccaactat tacgaccggg taagttcgtt
    35701 gcaggagagg cgtttacgtc ttccggtgcg gcggatactg gacatcatgc atcgttcgga
    35761 acttggcaag ccgctcccgg atgatttcac atttgagttt aacccgctct ggcaaatgtc
    35821 tgatgtcgat cgttcaacgg tggcattaaa taccaccaac gcaatcagta cagcgctggg
    35881 tgatggtctg atgacactga aagctgctat gactgatttg cgcgaaaatt ctgacgtaac
    35941 cggcataggg gcatccatta ccgacgagga catcgagaat gccgaagatg aagcgccgcc
    36001 cggcatcggc gaacctgatg acgaaccgca ggaaccgtca ggcggaaatc cgctatcgaa
    36061 ccagcctacg caggatagcg cgggcggtcg gggacatcgt aaatggtcgc tacgatggtt
    36121 caaatgacag tatcacggaa attattgagg cgctggaacg ctacagtgaa atcataaccc
    36181 cctgggcgac aaaggtcgcg gaaaacttta ccgccgacat tgtgcgcaag aatgatgagc
    36241 agtggcgtaa acacagcaaa accatcagcc gtgagctacg caatctggta aacagtgccc
    36301 ctccagggca ggtgatgaaa tccatcgttg ctgaacaggt taagtacatc aaatcgctac
    36361 ccctcgaggc ggctgacagg gtgtacgaca tccagaatcg ggcgattgat gctgttgtga
    36421 ccggtgggag agcggaacat tttgctaaag aaatagccgc atcgggtgat atagcaaagt
    36481 ccagagctga cctgattgcc cgtactgaac ttggacgtgc aaccggcgcg ctggatcagg
    36541 cgcgtgcgct gtcaattggt tcgaatggtt atatctggcg tacagccgaa gatggtgacg
    36601 tcaggcattc tcatcgggaa atggaaggta aatttgtcga atggggcaaa cctccaacgc
    36661 ttgatggcat gaccggtcac gctggcgagc tcccgaattg tcgctgttat aaagaaatcg
    36721 tttttcccac ctcccattct tatcccgcct gaatcgcagg taacacatga aatatttttt
    36781 caatacccgg ctgggggaaa cccgctatca gctggctgac ggctcgttgc tgtgcaaaga
    36841 cgtgccgata ggacgaacag gtaagcagct ctatggtgct gatgacctgc caaaactgaa
    36901 acccgataag ttcggtgaaa tagtcgtcac gcgttctcct gagcaggtat tccatcccgc
    36961 cacgcttgcc tcattcgaag gaatgagtat cacggtgttg catcccgagg atgaaaacgg
    37021 ggatgtgcgg ctggtgaatc cagagaactg gaaagagctc gcggtcgggc atcttcagaa
    37081 tgtccggcgc ggggcgggtg tgcagtctga tttgatgctg gctgacctta tcgtcaaaga
    37141 cgaaaacgcc attcagctga tcgaagatgg cctgcgcgaa gtgtcgtgtg gctatgacgc
    37201 ggaatataaa caggctgagc cgggtaaggc tcagcaggtc gatattaccg gaaaccatgt
    37261 ggctcttgtc tctaaaggca gagccggaaa tcgttgtgca attggagaca gagacacaat
    37321 ggcaaatcaa aagaaaagct ggtggacccg catgcgcacg gccatcaaaa cgggtgacgc
    37381 tgacaccatg aacgaactgg tggagtcggc tcccgcatcg gttacaggag atgaggggga
    37441 tttgccgcag ggcgttaatc tcaacatcaa cctgtccccg cagcaaccgc taccggacaa
    37501 agcaccagag atgggcggag gtccaaccgg cgacagtgat gatgacctca aaacattact
    37561 gaaagccctg ctggctaagc tggaaggaaa tgcgacgggc gataacgaca ataagcctga
    37621 cgataatccg accggtgacg gcgaggacga tgaagaggaa accacgatta ctggtgactc
    37681 agcctggcgt gccgaggtta tcgttccggg tatcgatctg agccgtaaga tgaaaccgac
    37741 cgcgttcaaa cgcgaggttc tggcttctgc tgacaaaacg ctggttcgcc agatagtcgg
    37801 tgatgcggat atccgcaaat taccgaaaca atcggtcgac atggcgttta atgccgtgtc
    37861 tgagattgcc aaagggcgaa acacccgcgc caccaccggc gatgcacagc gcccaaacat
    37921 gggcatgacc agtatcgctt ccctgaacaa acaaaacgct gaattctggg caaaccgtaa
    37981 agggtaaaaa atgaataatg tatttctgta ccggatgcct gttggcattg ccggggccgt
    38041 ctctcgcccg caggacttaa ccgtcgaacc ggtggtcctt aaatccgata acgccttcgc
    38101 tgcctatggg ctggctggta aatacgatga tgacggtttt ttcgtaccgc tggcagatgg
    38161 tgataccgca gacaaggtga aggggatcta cgtgcgccct tatccgacca cttcgcagcc
    38221 ggacatggtt cgccaggtgg ggagtggcaa gaacttcccg ggcgacgcca tgaagcgtgg
    38281 ctacgtgacc gttaatctcg gttctgattt tgatgccagc accatcaaaa aaggcgaccc
    38341 ggtatacgtt gtcgtctcca ctgatgaatc catcaaagtg ccgctgggtg gattcatgtc
    38401 cacgtcagtc agtggcaaaa atgtggtgct gaccaacgct gaattcacag gtgccggtga
    38461 tgctgacggc aatgcagaaa tttcctggaa gatttaagga acagacgaat gattactttt
    38521 gatcaggcaa ccgttgacag ctctggtgcc tttctcatcg gggagctgga gcgactcgac
    38581 cagacgctga acctgccact ggtggggtac acctggaccc gcgatattca gttgcgtgaa
    38641 gatgtctcca tcgcagatga catttccagc tggacgaata ccagcttcgc cgctgcgggt
    38701 actggcgcaa atccgaatgg gaaaaactgg gtaggcaaag actcaaccgc tattgctggc
    38761 gtgaacgtgg atatcggcaa atccggtaac ccgctgaacc tgtgggggat ggaacttggc
    38821 tggacggtca tagaattgca ggctgctcag caggtcggcc gcccgattga tacgcagaag
    38881 tatgacggta tgcaactgaa atggcagatg gataacgatg aacaggtata tgttggcgat
    38941 tcagcattaa acctgaaagg ccttgttacc ctggacggcg tgcctgtcaa caacgctgcc
    39001 aaaacgtggg caacctcaac accggacgaa atccgcgcga gcattaacca ggtactgtct
    39061 gatgcgtggg ccgcttccgg ttactctgtg gttccgcgtg atttgctgat cccgcctgag
    39121 cagtttgctc tgttgtccag catcatcgtt tcatctgcgg gtaaccagtc cctgttgacg
    39181 taccttcaga ccaacaccat cagctatcac cagaacggtg ttccgctgaa tatccgcgcg
    39241 gttaaatggc tgaaaggccg tggtgtgggg aataaggatc gcatggttgc gtacaccaac
    39301 gataaaaaat acgtccgcta cccgctggtt ccgcttcaga gcgtgccggt gcagtatcgc
    39361 ggtctgtatc agatcgtcac ttactacggc aagctgggtg cagtcgagcc agtgtacaaa
    39421 gaaacccttt cgtacgttga tggcatttaa cagccatatg gccccctggc ggggccatta
    39481 aggatgaccc gatggcaaaa aataatgcag taatacacgt acataccccg tttgtgctca
    39541 cgcttcccga cggttcgcgg cgcgagtttg ttaaaggccg tcatgctgtg gaggaagacg
    39601 ttgccacgca ctggttcact cgtgcgcacg cggaagtatc cgttggcaaa gccacagacg
    39661 cgcgtaacga ggtaaaaaat gccaaagaat caaagtctgc cagcggtaag tgattttcgc
    39721 cgcgacttcc cgcagtttgc tgaccctgcc aaatatcccg aagcgcaaat ccagtttcgt
    39781 ctgaatctgg ccgatgaact gctgagtgaa aacgtcaccg gcaaaaagtt gtttccgtac
    39841 tttgccggat tgttcgttgc gcactacatg acgctctggg cggcagacag cagagcgatg
    39901 ctggctggtg gtccgggcgg ttcaaccaat ggtgttcagt cctcaaagtc cgttgacaag
    39961 gtaagcgtca gctatgacac cagcgcgacg ctgaatcctg atgcaggttt ctggaataac
    40021 acccgatatg gcgctgaatt ttatcagttg atcacgatgt tcggtgcagg cggtcgccag
    40081 ctatgagttt caaaagcggt gtaacaacga gggtggataa cgctaaggcc attctggatg
    40141 cgctcaggtc gttaaccaaa aaagatgtgc tggtcggcat cccttcggaa gacagcgagc
    40201 gggatgatgt tccgtttggt aatgcgggca tcggttacct caacgaatac ggctcaccag
    40261 agcagaacat cccgccacga cctcatctgg tccccggcgt taaatcggca gaagagcaga
    40321 cggtgccgca gctcaaaacc gcggcgcagg ctgctcttga tggtaatgct gcgggagcag
    40381 aacgcgcact caaccgtgcc ggaacgctgg ccgctaatgg cgtcaggcgt tacatgacta
    40441 ttaccggctt tacgccgctt gctgacagca ctgttgaagc ccgggctcgt cgggggcgca
    40501 agggggcaac actggaactt gcccggcgtg ttgctggcga atctcccgga accgatctgg
    40561 cgaaaccatt aattgacacc gggcaatatc gcagagctat tacccatgta gtgagggata
    40621 aagatgccga ctcttgatgt aacagatgtg ctttttgacc ccgatttttg cgacttcaat
    40681 ttgtgggtaa cacgccgagt gcaaacggtg gatgaggacg ggatcggcag cgacagtgaa
    40741 gttgaaaagc agtttgccgg agtcgtaact gttgatcgct ctctggaaaa ccgccgtatg
    40801 caggcagggc aggtaatcag tggtgcaatt ctgattgtga cgactgagcg actgacgcag
    40861 ggacagactg gccgtgatgc cgatatcgtg acgtatcagg gccgtgatta tcgtgtgacc
    40921 ttcgtcgacc cgtatacagc ttatggggcc ggattcgttc aggcgcattg tgagttgatg
    40981 ccgtttgatg ggggaactcc ggttgagcaa taacaccagt acagagcgcg gatggttaat
    41041 accaaccagt ggcgatccgg attatgacga agcgctcgac aggcttttaa gccagtggat
    41101 gcgtaacgtt tccggtctgt ctgccgggat ggttcgtccg cgctggcaga aagagcaacc
    41161 gccactgcta ccggctgaaa cgaactggtg tgcgtttggg gttatcggat ggtcaggtga
    41221 tgacagtccg gcattcacca gacagaccga tgatggctct cagctctggc ggcatgaaac
    41281 gattgagtgt atggcttcgt tttatggtcc ggcggggatg gtgtatgcgt cccggtttcg
    41341 tgacggtata tctgtaccgc agaacaacgc agcactgaat gcgctggggc tgtctcttgg
    41401 cgattacaca ggtctgactc ccttccctga acttattaat cagcaatggg tccgccgcta
    41461 cgatatgacg gtgcgtctgc gccggaaggt tgtgcgcgag tacggtatta aatcgctggt
    41521 ggaagcacca gtcatctttt tcggagatta agctatggca cagggcttgc ctgtatcaaa
    41581 cgttgttaat gttgatgtga tcatgtcgcc gcgtgcagca tcagggcgaa attttggtgc
    41641 attactcatt ctcggcccgt ccacaatcat tccggtaagt gagcgcattc gccgttattc
    41701 tgccgcggaa gatattggaa aagattttgg cgtggaatca ccagaatata aggctgcgca
    41761 ggtgtttttc tcacaatcac cgaagcctca ggaggttttt gttggtcgtt gggtgaaaac
    41821 gaagggagac agcgaacagg ccacgcctga gacgctggag caggctgtga atgccatgct
    41881 tgattatact tcatggtatg ggctggggat tgcagacgat gcagatattc cggatgcaga
    41941 ctggctgaaa gtggctgcgg cgatcgaatc ctcttctgta agccgtattc tggcgattac
    42001 gacaagcgat gagaaatgcc tgcagactgc atccagcgat gatttggcat caaaactgaa
    42061 aaccgccgga tattcacgca gttttattca gtattcatcg ggtaataaat acgctgcgtt
    42121 atctgcattt ggccgggcat tcacggttaa ttttaatggc agtaataccg cgattacgct
    42181 caagtttaag caggagccgg gtgtcgggta tgaaacactg acagtcagcc aggcatcggc
    42241 acttgatgca aaaaactgca atgtattcgt gtactaccag aatgatacag ctatcctcca
    42301 gcagggagtg atgaccaacg gtgatttctt tgatgaacgc cacggcctgg actggttaca
    42361 gaattatgtg cagaccaacc tctataacct gctttatacc agcaccacga aagttcccca
    42421 gactgaagcc ggtattaccc gactgttatc aagtgttgaa aaatcactgg atcaggccgt
    42481 tcagaatgga ctgattgctc cgggcgtatg gaacgggggc gaccttggcc agttgtcatc
    42541 aggtgacacg ctgcccaaag gttattacgt atacgcccag ccgctggatg aacaggcaca
    42601 atcagaacgt gaagcccgta aggctccggt gattcaggct gcaataaaac ttgcaggcgc
    42661 ggttcattac gctgacgtac agattaacgt tgttcgctaa ggggaagtga atgtctacct
    42721 attcttttat ggatgtcact gcgacgctga ccgggccgac cggttcgatt gacctcgggt
    42781 acggttcggc aagttctgaa gaggggattg tggttgcgat gggcggtcct aaaaacacca
    42841 tgaccatcgg tgctgatggc gaagtgatgc acagtctcca tgcagataaa agcgggacga
    42901 ttaccgttaa ccttctgaag acatcaccga caaataaaaa attgtcgctg gcgtataacg
    42961 cacagagcca gtcttctgcc acatggggga ataacgttat cgtgatccgc aacaaggtca
    43021 gcggcgacat catcacggca cgcagtgttg cgttccagaa acaaccggat aacgccaacg
    43081 ctaaaaccgg taatacgatg ccgtgggtgt ttgactgcgg caagattgac caggttctcg
    43141 gggagtttta atacatggaa ttcgaaatca aaggcgtgaa atatcgcgtg gcaaaactca
    43201 gcgtttttga ccagctgaaa gtgacccgca aacttctgcc ggtgctggca ggaatgatgt
    43261 cagatttcgg gagcattcgc tcccgtttgc ctgctgatgg caaaatcgac accgtgaaat
    43321 tcgagcagtt aaaaccggtg tttgaaacca tgctcccgcg tatcgctgag gaactgtctt
    43381 ccctgaccga agatgacacc gatgcgatta ttcatccctg tcttgcggtg gtatcgcggc
    43441 gtcatatgga cggatgggtt ccggtattta cccggggcga actgatgttt gatgatattg
    43501 acttgctggt catgctgcag ctggtggcgc gggtggtcgc cgattcgctg ggaaattttt
    43561 tgcctacacc ccttaccagc acgacgcaga gcctgcaaca gggctgacgt ttaacagcct
    43621 gccggacggg ctgtcctacc ttctcaatcc ggttgacgcc gggttaattc cttatacagc
    43681 acttaaagat ggctctgtcg atttgtacga tattgctctt ttgaatgacc atctggcggt
    43741 aaaagcggat aaccagcggc gcattgagaa atggagagag gataatgaac gctgaaacta
    43801 ttaaagattt cctcgtctcg cttggcttca gtgtggatga tgcaggagcg aaaaagttcg
    43861 gttctgtcct cgccggtaca actgcaaatg tcatcaaaat ggggctggcc gttgaaggag
    43921 ccgcgctgtc cgtggtggcc ttcacggcta agatcgcctc cggtctggat aatctttact
    43981 gggcgtcaca gcgcactgga gcgacagtcc agggaattca gtctattggc tatgcggttt
    44041 cgcaggttgg cggcagcgtg gacgctgcgc gatcttctct ggaaagcctc tcccggttta
    44101 ttcgcaacaa tcccggtgca gaaggctttc tgaatcgcct gggcgtacag acccgtgatg
    44161 ccagcggtaa catgcgtgac atggccgcta tctttacggg cgttggacag aaactcagta
    44221 gcatgccgta ttaccgggct aaccagtatg cgcagatgct gggtattgac gaaaataccc
    44281 ttatggcgat gcgtcggggt gtgggtggtt tctccgggca gtacagcgca atggcgaaag
    44341 ctatcggctt caatgctgac gaggcggcca gaagctccaa caaatttatg acctccctgc
    44401 gtgagtttag cgcgatggca ggcatggccc gtgacaaaat cggctctaat cttgcgggtg
    44461 gacttgcggg ttcgctggac accctgcgcc gccatatcct ggacaacttc cctcgtatcg
    44521 agcagaccct gacgaaagcc ataaaaggca ttctggcgct cggggatatt atcgggcggc
    44581 tgttcttcag actgattgag gggacatcag gccttatcac ctggtggcaa tctctggata
    44641 agcaaacgcg ggagttgatc tcgctgtttg gcgcactgac gattgcgctg cgcattctga
    44701 acagtacgtt ctggatgtcg ccggttggcc tcattaccgc gctggcggcg gggattgccc
    44761 tcctgtggga ggactatcag acctggaagg aaggcggcga cagcctgatt gactggggca
    44821 agtggaaacc ggaggtcgat gccgcgctga agatggttcg tgaccttaaa acgaccgtta
    44881 acgatctggc gaaagcgctg gcgaaactac tcaatattga ccccaaatca tggtccctga
    44941 agtgggattt cagcaacttc atcgaccaga tgggcgaatt cagcaaaatg ctgaacatga
    45001 tcgccgacct gctcaacgct atcaaagatg gacgctgggc tgatgccgtc agcatcggca
    45061 aacagatact taatcagggc agcgaaaatc cgtcagcgat gccgatggtt acagacagcg
    45121 ctaacagtac tgccgactgg attaaagagc actggggatt cgatccccgc agcgtgggcc
    45181 ggacggtacg cggctggttt ggtgatgatg agccggaaca acatgcacag gctacgaaac
    45241 gaggagaacg gaataacaat ccgggaaacc ttaattttgc tggtcaggca ggggcttctc
    45301 ttgaacgccc gggcgggcga tttgccagat ttgaaactgc ctttgatgga ttacgggctc
    45361 ttgctcgtca gttaatgctg tacgccggac ggggaataaa cagtgtggag aaaattatct
    45421 ctacctgggc acctgcgtct gataataaca acacaactgc gtatatcagg gctgtatcgc
    45481 aacgactggg agtggatccc cgggctgccc tgaatatgag cgatccgcaa accatgtcag
    45541 cattgatgag cagcattatc cagcatgaaa atggaagaaa tatctattct cgagagctga
    45601 ttaataaggc tgccgtggcg ggaattagtg gcaaaatgac agaggttaac cagcaaaata
    45661 cttaccacat ttacggtggc ggagatccgc acgctgtcgg taatgaggtt gcacgtcggc
    45721 aacagtctgc aaatgctcag gtcatgcgaa gtaatcaggt gagggtgggt tagtggatat
    45781 tctctctaca ctttttcatc agcagagcag aaaaatagga atgattgttc ccagtgttgt
    45841 tatttcagag aagcatacag atatgcttga aataacagag catccggtag aggttggggc
    45901 cgctgtcgct gatcatgcct ataaaaaacc gtcagaagtg gtgatggagg ttggtttcgc
    45961 cggtggcggc gcattgctgg attttgccag taatctgacg gctaccagcc tgctcggcct
    46021 gagtcctcag cagacgtatc aggagctact ggatctgcag gaaaaccgta tccccttcga
    46081 tgtggtaacc ggtaaacgac tgtacagcaa catgttgatc cgggcgttgg aagtgacgac
    46141 ggacaagaca accgaaaacg tcctgtccgc cgtcctcacc ctgagggagg tcattatctc
    46201 ccggacacag cagattaccg tcgcggataa aaccaacatg aaggaagggg ccagcacgtc
    46261 gtcggtacag aacagcggca acaaaaccac aaagcctcca gatacttcac tgctgaaaag
    46321 catcacgggt aacgtggcgt cattactggg gggcggctaa tgacaattca ggaaattccg
    46381 ctgacagcgg acaaccagca gttcagcatc gtcctggggg gggtcacctg gcggattagc
    46441 atcatatggc gcgatctgta ctggattatg gacctgcaga acgacagagg ggagccggta
    46501 atctccggta ttcctcttgt cactggtgct gacctgctgg cgcagtacgc ctgtatggag
    46561 cttggtttta agctggtggt ggtctgtgat gacaacacac aggattaccc cacaaaaact
    46621 gacctgggcg gtcgcagcca tttactggta tcaacggagt aagcatgtca cagaactgga
    46681 tgagacattt cgagctgcag cttgtggacg ggaacggtca gggaattgag ctaagtgatt
    46741 tcaaagtcac ctttacgatc gactggttca acatcagcag cgcgtcccgg gtagggacta
    46801 tcaaaattta taacctctcg gcagatactg tgaaccgaat taccgggcag gagttttcga
    46861 aagtgcgtct gattgcgggt tacgacggta tcgcgccgga ggtgtcagca agcgacgtag
    46921 ggaccgtgcg ggaagttgac gcggcggacg tgggccagag agatggccgc aactacggac
    46981 tgattttcag cggtgaaatt cgctactcgg tcacaggaaa agacagtccg gttgatacct
    47041 acgtcctgat tcaggcagca gatactgatc tggcttttgc caccagtata acctcacaga
    47101 cgctggctgc cggttacacg gtcgctgatg tgaaccgtgc gctgatgaaa gacttcgaag
    47161 ccaaaggcgc gaccgaaggc ctgacgcctg aaatgcctgc tactgtattc ccccgggggc
    47221 gggtactctt tggcatgacg cggcatctaa tggataacgt agccggacaa tgtggcgcaa
    47281 catggcaatt cgtggacggt cagcgccaga tggtggcgaa taacgaatat gttcacgaag
    47341 cgattgtgct caacagcgct accgggctta ttggcatgcc gcagcagact atcggtaacg
    47401 gcgtaaacgt ccgcgcgctt attaatccga acatccgggt taacgggctc attcagctgg
    47461 atcaggcttc cgtctatcgt accgcgttgt cgaacaacga tattgcgatg gttggtggtc
    47521 agatcaccga ccagaacacg gacggaaata tcacgctcag cggcaccaca tcgcagcctg
    47581 ccagcatcgc aacggatggc gtttatattg tgcgcgggat tatgtacact ggcgacacaa
    47641 ggggccaggc gtggtacatg gatatgatgt gcgaagcgcg tggcgcggcg gatctgtata
    47701 cgcaatcggc tttgcaaagg ggatgagcaa tgaggggtat tatttttctg ttagctgcct
    47761 tttctgcgtg cagcgcgtgg gcggatggct tcacggttaa atgcggtggc tacactatgg
    47821 ttgcaaacca gggcgagtta tcgactatca acggtgaaag agttacctct caaaaaatca
    47881 ccgaactggg taccaatggt ttgaaagtag acatggggct tatgcctgcc aaagacggta
    47941 acaactacgg ctttgaatac attcgtcgcc ctggtaccga aacgcgattc ctgaatgtcc
    48001 agcttctgca gaacagcatg gatgcgccga aaatcatcgg atctttccct tgtaaaaaag
    48061 tggctgggcg atgatttttg acgtccataa tattatggat gaactgttgc caattgcgct
    48121 aaatttaaca aaataatact ggttaacgca ttttttgaac catctgtaca cagtagttgt
    48181 ctatacttta ccttaaaggt aaactctgcg ctccactgat gttcactatg ggaggggata
    48241 tgaggaaaat cgttaatcgt tacttagccg ctgccggtag tgttatggac attatgccga
    48301 ctacagatta ctggcaatat gctgcttctg gatctgattg tcgggcaata agaagtgatg
    48361 ttaaagtcat tgcaagtgac atgaacagag ttatcaggta tgagttcaaa accccgcaag
    48421 caggcggccg ttctcaaaaa gcatcaggaa aacaaagcgt cgcaaaaagc cgttcctgat
    48481 tcggatggta tcgacgaaga tattgattcc tcggaatatg atgagtctca ggaagtagtg
    48541 gaaaagctgt ctgcagttgc caaccttgat cctgagctaa aatcgaagtt aacacctgaa
    48601 cttcttgaga gagtttctaa gaacccgaag gttattgcca tgtttcagca ggagtctttt
    48661 cagggaccac tcccacctcc tgctatgctc ttacaatata atcaaatatt acccggcggc
    48721 gctgagagaa ttttcaggct aaccgaaaaa gagcaagagc ataggcataa gacgcaaagt
    48781 gatgcattac ttggtgcggt ttcacgcgac aaacgtgggc aatggatggg attttgcatt
    48841 gcaatggtag tgcttataat ggctgcgttt tttgctaatc gcggtaacac ttggttcgca
    48901 ggtactcttg ctactttaga tctcgtagga ttaactacag tgtttgttta tggtaggaag
    48961 aaacctaaat aatccataca tcctttcaaa cccgcttcgg cgggtttttt gctttctgga
    49021 gcctactaaa tggccgtatc tgaccagacc cgcagcggcg accttgccga aacattcaaa
    49081 tctgaacggg aaacaacaaa gaaccagatc cgtgtcgcct tgcctggcat tattcagtca
    49141 ttcgctcctg atgcggtgac ggcggttgta cagcctgcta tccgttacgt tgaaattgat
    49201 aacgacggca accgcattac caaaaattac ccattgctgg tggatgtgcc agtggtattc
    49261 ccgcgtggcg gaggctgtac gctaacgttt ccagttaaag ccggtgatga atgtttggtg
    49321 atttttgccg atcgttgtat tgatttctgg tggcagagtg gcgggataca ggagccggtc
    49381 gatgacagaa tgcatgattt atcggatgcg ttttgtattg tcggtcccca gtcgcaggca
    49441 aggaagatta gcggtattaa taccagtgcc acacagttgc gtagtgatga cggcagcacc
    49501 tattttgagc ttaatcctga taccaggaaa attaaaattg tcgctccggg gggccttgat
    49561 gtggttgccc ctctggctga tttttctgag aaagtaacca ttcatggcct gttaacctgg
    49621 atgggaggca tggtggggtc tgttgtttct ggtgtggctt caaaaatcac tggtgctgtt
    49681 gagtttttgg gtagcgtgaa ggctaacggc aagccaatcg atgatacgca cactcatggt
    49741 ggtgttcagc gcggtggaag caataccgac ggagtaaact gatgcgatac agacgtgaag
    49801 acgccgatgg cgattacacc tttggcagcg gtgatgacac ctggctgatt aactcaccgg
    49861 aggccgtggc gcaggcggta aaaacgcgat tcgaattgtg gtatgggcaa tggtttctcg
    49921 acaccactga ggggactccg tggatccagt ccgtactcgg taagcagaag ccggaaacct
    49981 acaacctggc gatccgtaag cgcattctgg aaacgcaggg cgttaaatca atcctctctt
    50041 tcaatacgac ggtggatacc acgacccgac gtgtcatgtt ttccgctgaa atcgacactc
    50101 tctatggaat aacgactgtt acatcggagg cgtaatggct ctgaaccttg attctctcgg
    50161 tttatctgca aaggtaaccg cggaggggat cagtgcgcct gattatcaga cgatactcag
    50221 caccctgatt agctattttc agcagattta tggcagtgat gcctacctcg aaccggacag
    50281 caaagacggc cagatggtgg ctctgatggc gctggcgatt catgatgcca ataatatggc
    50341 gataactgtc tacaactgtt tttcaccggc aaccggctat ggggctgcac tgaccagtaa
    50401 cgtgaaaata aatggtattt cacgtaaagg cgcgacgaac tctacggttg atttgcttct
    50461 tacaggaact gccggaacaa ccatcattaa tggcagcgtg aaagacagta ataatgtgat
    50521 atggcgtttg cctgcttcag tggtggtcgg cgtggatggt acagtgatgg tgaccgcaac
    50581 atgttccgtc agtggtgcag tggcggcgct ggctggaact atcactgaaa ttaatacgcc
    50641 aacccgtggc tgggtttcgg taactaatcc tgctgcggct actgtgggca ctccggcaga
    50701 aactgacgct gagttacgta tccgccagtc gcaaagtgtt gcgttgccat cattaacccc
    50761 atttgaagca ctggatggtg ctgtttctaa tgttatcggt gtaacccgcc acaaactcta
    50821 tgaaaacgat actggttcgg aggacggtaa cggattaccg ccacactctg ttgctgtaat
    50881 tgtggatggc ggtgatgtga cggatattgc tcaggctatc agagggaata aaggccaggg
    50941 gacagccact cacggtacaa catccgttac ggttccggat aaatacggca atccccatgt
    51001 aatcaaattc tcgcgttcca gtgatgtgcc tgtttatgcc cggattaaat taaaagtttt
    51061 tacgggttat acctcacaga taggacagca gatccagcag gctatttccg actatatcaa
    51121 tagtctgacg attggtgatt cggtcctttt gagtcgcatt tactcaccgg cgaatcttgg
    51181 cgtggtgagt ggcgggaatg cacgctatta cgatattcag gaactgacga ttgggaaatc
    51241 cccgggggct ttgtcgtcat caaacattga tatcagatac aacgaatctg cgtcctgtac
    51301 cccggaaaat atcgttataa cggtggagtc atgagcaaat acaccgaact aatcacgaac
    51361 taccacgcca ccaaacctaa atttcttgca catgttgatc tgatgacccg gccgcttatt
    51421 gatgttgcgg ctgccaccag agggctgatt actgtatttg atattgactc tgcggttggt
    51481 gtgcaacttg acattcttgg attgtggatc ggacgtagcc gtgttgtcag ccagcctatc
    51541 tcaggtgtct atttccgctg ggataccgac gggcttggat atgatcaggg ggtatggcag
    51601 gggccatatg atcctgattc aggatacatg tatctcagcg atgaaactta tcgtgtcatt
    51661 cttaaagcga agattgcgat taataactgg gacggacgga atgattcgct tccggcaatc
    51721 cttgacgctg caacagcagg atccggactg cgaatgcaga ttgtcgataa ccaggacatg
    51781 acgatatcgg tctgggtctt tcctgatact gatatttcag atgtatcgcg tgagttaatt
    51841 gctgcaatta aacagggata tctcacagta aaagccgccg gggtgtgggc gggtggcatt
    51901 gaaacacctt cggtggaaac cccatcggaa ggttcaaaat tttttggttt tgatatggat
    51961 aacgaattta tcagtggttt tgatctaggg gcatggggag tattactctg atggcaaaaa
    52021 atgactttaa agcattcgca acgggtaaaa atgccaatgt tatgtcgcag gaggaatggg
    52081 aagcgttgcc tgcgctttta tctggattta cagcagggaa agcctccagt gcgcaagtca
    52141 ataaggttat tcggcaggcc agctttattg ctgcagctct ggcccagttt gtaagtgata
    52201 aaacgcaacg ggatgtgctt gataatggtg atctgcccgg ttttgttgaa ttgctgggat
    52261 cggggtttgc tgttgaatac ctgagccgca agaatccgtt tggtgatatc aaatcggatg
    52321 gcacggtgaa aacggctctc gaaaaccttg gtttgggaga aggttcagca ttacctgttg
    52381 gtgcacctgt tccgtggcct tcagagacac ctccaacagg ctggctgaaa tgcaacggag
    52441 cagctttttc tgctgaagaa tacccggaac tggcaaaggc ttacccgaca aataaattgc
    52501 ctgatttacg tggtgagttt attcgtggct gggatgattc tagagggatt gatacggggc
    52561 gttcattgct aagtggtcag gctgcaacat ttattcgtac agctttgcag gattattacg
    52621 gttacgatct gaatactaat gtcaaagtag gtatcgcttt tgctactgct gattctgtta
    52681 taactgttgg aaacccagct aatcctaaag caggaaataa tagcgattat gttcctgcat
    52741 cagcagataa ctccataacg ggtactcaaa ggacagcgga ggataatttt accggggcat
    52801 ggatatcaat gcgccctcgt aacctttcat ttaattacat cgtgagggct gcataatgga
    52861 taatgccgta ttaaatagcg agtttattgc cacgaaggcg ggggatatta ctgtctataa
    52921 ttataatggt gaaacacgag aatatatttc cacatcaact gaatatcttg ctgttggtgt
    52981 cggtattccg gcatgttcct gtttagatgc accaggaaca tataaagctg gttatgccat
    53041 ctgccgctct gcggatttaa actcatggga atatgtgcca gaccatcgcg gagaaatcgt
    53101 ctataacacc gaaacgggag acgccaaaga aatcacaact ccgggtgatt accccgaaaa
    53161 tacaaccact atcgccccat taacgccata cgataaatgg gatggtgaga aatgggtgac
    53221 cgataccgag gcacagcata gcgccgcagt agaagcggca gaagcacagc gccagtcgct
    53281 gattgatgct gcaatggctt ccatcagtct gattcaactg aaattacagg ctgggcggaa
    53341 gctgacgcag ccagaaaaca cccgacttaa cgctgtgctg gattacattg acgcggtgac
    53401 ggcaacagat accagcacag cgccggacgt catctggcct gaactgccgg aggcgtaggc
    53461 cattcaatat ctggcgcacc ggaagtatcg accagttcca gtgcgtccag ataatccagc
    53521 cacaaattat attgcgccag ttcctcacct ttcagacgac caattgatgc tttacctggc
    53581 cattgtttac tgttgatgta ttcgttggcc tggttaatca attgctgctt tttagtttcg
    53641 gctgatgcaa tttgttcttc acgtgttggt ggaggaatat ctgcccatgc aggcagtcca
    53701 tcctctccga cacatctgta ttttccttct ggtggtgtat catagaaata ttccctgaaa
    53761 attacttcgt ctatatcaac acctttttct tcaggccatt cacctttttc aacataaaga
    53821 gactgaagtt cgtaaggata tgccaggttg tttgcgtaca gatatttcat cattaccagc
    53881 ccttagcgaa aaacgcacca ccttcaagac cataattgca gtgagctatg aagccggtag
    53941 tgctccagtt ggtcgccccc cacatattcc cgcccccgaa accaccatcg cacacaatta
    54001 caatgcccgg tgtctgtgta aatggaattg ggaatgaaac attggcggat acaggcccgt
    54061 gttcaccagg aaaactaatt cgtccccact gttcaattga accatctggc atttttcgcc
    54121 agcctgaacc tgatgcatat gatgacatat caggtatctg attttcccct gttcccacat
    54181 ttcgttttgc cgcttctccc aaaccaacgt ttaagaaaat gcagagatta cggctaactg
    54241 gcatcatccc cggtttttat tcaggggatc catcatgctt attggctatg tccgcgtatc
    54301 aacaaatgac cagaatacag aattgcagcg taacgcgctg gagtgcgcag gatgtgaact
    54361 gatttttgaa gataaaatca gcggaacaaa atcagccaga ccgggattga aaaaactgct
    54421 cagaacgcta tcagaaggag atccgctggt tgtctggaag ctggacagac tggggcgcag
    54481 tgtgcggcat cttgtcgtgc tggtggagga gttgcgcgaa caaggtgtta attttagtag
    54541 ttatgttatt acaggtgggg gatttctccc ccttatgcag attttctcgt taactattgg
    54601 cgaagtggcc atatttttct gttttttaaa aaactatctt caactatata actgcaacaa
    54661 tgttcactaa ggccataaaa actaattatt ccatatttat gagaagctat atcagacagt
    54721 tctgctaata ctgtggcaag ccaacgagcg atgctaagag aatagaatct tccccacttt
    54781 tgaacaatgg tattttgtcc agtacggatg gaggctgata taaggtcttg cataatgttg
    54841 gtactttcat tcatatacat gaccgttgta tacggtgaaa gagtggcatt gataaaccta
    54901 gcattttctt caacacgtct ttgttcaact gtataataat aatgtttttc aaggatactc
    54961 tcggctactt ctccccacca tttattaata ggttcattat tggttaaatt ggggctgctt
    55021 aatgaggaga aatttgcgta acgtccccgt cgggcatcag caaaggaatc taaacattca
    55081 agtattttgt aggtaatttt atgtgtagga cgtgaatatc tcagagatag ttgcattttt
    55141 ttagaaatac tatcaacctc gttggttaat tcaatgagct tgtgtccaaa ttttcttaca
    55201 atattctcgt caggcatttt tcctttgtgt tcaatggcat agtgtgcaac aagtattagt
    55261 ttagcaagtc tctcaaaacc tacagaaagc ccaaaaaatg ctgtgtaata ttcacctatt
    55321 ttgtctgcat aattggcttt acctatggct gttgccccag aaccaatgag ctgtgttact
    55381 aaagatgctt ctcggcccag agcgtgccat gctggaatat cccagggatt tttattcact
    55441 ttattatttt cctttgctca taaatttaaa ttgtttgagt atctcataaa atataaggag
    55501 gtggttaagg ttgttgatcg catataatag ctattgaaag tcaagaactc ggttttatgt
    55561 tctaccacta attaatatat tgtgagtctg acaatatatt aattagtatg atcaaaccag
    55621 ccatcaactg attccttgta tctttcagag tctccagaac aaaagcctta acgaattcct
    55681 tatttgtgag tgcattacaa aaagccattg ttgcagggaa cgagtataaa actcgccagc
    55741 tactggagca ggctacctga ttgtcgatcg attcctgcgt ccatatagtc gatggtcttc
    55801 tggagctctt cgattagggc aacagctttt gctcgagaaa tacatattag ttgatccgga
    55861 aattcttgta agggccagtt tggtatacag gccatgttat cagtgaagga tgcagacaag
    55921 taaacttcat tggtaaccag agaatagctt acctcaaaat tggtgagttc cggcaaactg
    55981 ctaacgtttg actcttttct gcgcataata ttatccatga ctgtgattat gtacaggtga
    56041 tggtcttcaa tttcgaagag ggagtcaagg gggagtctca aatgaaccgt tatagaaagg
    56101 gggaggatct ttgtgttgct gaacttatgc aaaatttttt tgcaccataa atccaacaac
    56161 acattcaata acacattgct gtatcactat gaaaataaat gtgtttttat ttgatttcac
    56221 acatatcaca attgatgcat tttttagtga ttaacaacgc cataaggaaa ctctcaaaac
    56281 aacacaaacc gggcgggcat tatacgcgca ttctttactc agaccagttt tttcaccagc
    56341 gcttcactat gacgaatacg ttctggtgcc agttccagat cctgctgaat cagcgcaatg
    56401 aacagcaaat ccgttaacat tccctgagcg tgacaagcag agattgaagc actgtttgtc
    56461 gcttgttctt cggcaatggt atacaggcaa tgagaagcac gctgttgcag ggcattcgga
    56521 gtaaagccgg taatcgccag cacttttccg cccactcgca gcatctcatc tgccgccagg
    56581 tttaactcgc gtcgtacacc ggtgtaggaa atggctaata acagatcgtc aggggacgac
    56641 gcctgtactg ttgcgagtag cgcatgcata tcgcgcactg cggcagcatt gaagccaatc
    56701 ttcatcagct tccaggcaaa gttttgcgcc accagacccg aagcgccaat accggtcaga
    56761 attatccgcc gcgcagagcg caacattgtt acgcattcat acagtttctc ttcactatta
    56821 acgtttagcg ttgcgtacat agcggcagta ttttctttaa tcagtttttc gccgaccagc
    56881 cgtaacggat catcaccgcg gatttggtta tgaatgggca cggagggcga ttccggctgg
    56941 cttgccagcg cttcactcaa cgccaactta agcgccggaa aacctttata gccgagtttt
    57001 tgcgcgaact tcacgacgct ggactgactg actccggctt cgttggccag ttgctgagag
    57061 cttaaatggc gcgccgtatc aggttgtagc agcagataat ccgccagttt tttatcactc
    57121 tgggcaagcc cctggtaacg ctgacggata cgaagtaagc cgttcatatc tttcctcgcg
    57181 atgaaatagt ggcgacaaca ggcaatatga gagacacgcg agtgaatttt atattccatt
    57241 gccgctaatt gccatgaatt aaaaattcca gttagccatg atgcggtcga ttgttgccgg
    57301 aaaagtgatg atctatctgt taggctatgt agtagcgaaa attaatcagg atgtttcagt
    57361 ccagaggagt atggtttggc aactcacgag cgtcgtgtgg tgttttttga cttagatgga
    57421 acattgcatc agcaggatat gttcggcagt tttctgcgct atttactgcg tcgccaaccg
    57481 ttgaatgcgt tacttgtcct gccgttgtta ccgattatag ccattgcgtt attgataaaa
    57541 ggtcgtgcgg cacgctggcc gatgagtctg cttctgtggg ggtgcacttt tggtcacagc
    57601 gaagcacgtt tacagacgtt gcaggccgat ttcgtgcgct ggtttcgcga caatgttacc
    57661 gcctttccgc tggttcagga gcgattaacc acctacctgt taagttccga tgctgatatc
    57721 tggttgatta ccggctctcc gcagccgctg gttgaagcgg tttatttcga tacgccctgg
    57781 ctgccgcggg ttaatcttat cgccagccaa attcagcgtg gctatggtgg ttgggtattg
    57841 acgatgcgtt gtctgggaca tgaaaaggtc gcacaactgg agcgcaaaat cggcactccg
    57901 ctgcggctgt acagtggcta tagcgacagt aatcaggaca atccgctgct ttatttctgt
    57961 cagcatcgtt ggcgagtaac cccgcgcggt gaactccagc aactggaata gagtaaagca
    58021 tagcgtccgt gtataatgcg ccgcgctttt ataaccggag ttttcttttt gtctgaagtc
    58081 gaatttagcc acgaatactg gatgcgtcac gcgctgacgc tggcgaaacg tgcctgggat
    58141 gagcgggaag tgccggtcgg cgcggtatta gtgcataaca atcgggtaat cggcgaaggc
    58201 tggaaccgcc cgattggtcg ccatgatccc accgcacatg cagaaatcat ggccctgcgg
    58261 cagggtggtc tggtgatgca aaattatcgt ctgatcgacg ccacgttgta tgtcacgctt
    58321 gaaccatgtg taatgtgtgc cggagcgatg atccacagtc gcattggtcg cgtggtcttt
    58381 ggtgcgcgtg acgcgaaaac tggcgctgcg ggatctttaa tggatgtgct gcatcatccg
    58441 ggtatgaatc accgagtgga aattacggaa ggaatactgg cggatgagtg cgcggcgttg
    58501 ctcagtgact tctttcgcat gcgccgccag gaaattaaag cgcagaaaaa agcgcaatcc
    58561 tcgacggatt aattttgttt ctcttcactc cctttcctgg aaaacagcag agagggagaa
    58621 tgggtcaaat agtttgatga cgactgggaa agaaacgaga gaaaaggaaa tttctctttg
    58681 cccaactccg taggcgatac cgccggataa tcctgcgcca gttgcatcgc cgcttctgta
    58741 gcctgcttct ctttctcttg cagataaccc accaggctaa tctgatactt acgaatattt
    58801 tcgacataag cgtaggcttc atgtccacga gcgtagccgt aagtcagctt gctgtaatag
    58861 ggtttctggc taagtaaagg cagacgctgt tttacgtcag cccaactgtc aggattccct
    58921 ttggtttttg ccgtcagggc gcgggcatcc agcatatgcg catagcccat attgtacgca
    58981 gcgagggcaa accagatccg ctcgttctcc ggcacacttt ccggcacttt actcatcata
    59041 tcctgcaaat aacgcacgcc accgctgatg ctctgttcgg catcggtacg atccgtaatg
    59101 ccgaggcttt gcgcggtatt tttggttaac atcatcatgc cgcgcacacc cgtcggtgaa
    59161 gtggcctgtg catcccagtg cgattcctga taagcaatag cggccagcaa acgccagtca
    59221 atttcttcgg cgtatttctc aaacaggggc tttaactgcg gcagtaccgc atcgacggcg
    59281 cgtaaaaatg tgcgcgtatc gacgtaatca aaatcatcgc catgccctag gtatttctct
    59341 tcaatgcgtg ccagcgtacc gtcttcattc atttcgttga agaagtcgag cagggcggcg
    59401 gaaagggtat tatcgccatc taacgggcta aaccaggtca ccggttgttc atcggtgata
    59461 tcgagcgcta cggcgagctc cgggtgaacg cgctgaaaca ggctgatggc gacagaatca
    59521 gcaatggtgt aatcgagttt tccttcgatg acatcttcca ttaattccgc agagcctttt
    59581 ttgtcgtcta ccttccagct taattccggg aattttgtgt ctttcagggt ttggagatcg
    59641 ttaaccacca cgtgacccgg tgcaacggtg agttgctccg ccgtcaggtt gcccagtgtg
    59701 cgcgggcgat actgacccac tttataaacc agttgttgtg acacggaata ataggtaggg
    59761 ccaggctgat aattttttac ccgctcactg ttatagacca gtcctgccgc cagcaggtcg
    59821 gcgttaccat tatcgaggtc gtcaaacagc tggctgatat tctgccgcac ggtcactttc
    59881 agttttacac cgaggtaatc ggcaaactgt ttcgccagtt cgtaatccag gccaaaaggt
    59941 ttcccgttga tttcgttata agtcagggga gtatgaatgg tgctcacacg caactctccc
    60001 cgcgcttgaa tggcggcgat acggttgtcg gctttaccaa accagggaat ggatggccag
    60061 agagcgaccg cgagcagcag tgccagaatg ccgatgaaca gataattaat ctttaatttt
    60121 ttcaattagt taattctctg tgtcgtgcgc gtcccagctt gaaaaaacgt aataatagtg
    60181 aaaggtttac tcataaatga gcggcatttt gcgtaaacct gcgccagatg gcaacttatt
    60241 acagccattg gcggcacgcg ttgctaattc acgatggtga ttttatttcc acgcaaacgg
    60301 tttcgtcagc gcatcagatt ctttataatg acgcccgttt cccccccctt gggtacaccg
    60361 aaagcttaga agacgagaga cttatgatgg aaattctgcg tggttcgcct gcactgtcgg
    60421 cattccgaat caacaaactg ctggcacgtt ttcaggctgc caggctcccg gttcacaata
    60481 tttacgccga gtatgtccat tttgctgacc tcaatgcgcc gttaaacgat gatgagcacg
    60541 cacaacttga acgcctgctg aaatatggtc cggcactcgc cagccacgcc ccgcaaggca
    60601 aactcctgct ggtgaccccg cgtcctggca ccatctctcc ctggtcttcg aaagcgaccg
    60661 atattgccca taactgcggg ctacaacagg taaaccgcct tgagcgcggc gttgcttact
    60721 atatagaagc cggtacgctg accaatgaac aatggcagca ggttaccgct gaactgcacg
    60781 accgcatgat ggaaacggtc ttttttgctt tagatgatgc agagcagctg tttgcacacc
    60841 atcaaccgac tccggtcacc agcgttgatt tgctggggca gggccgtcag gcgctaatcg
    60901 acgctaacct gcgtcttggc ctggcactgg cggaagatga aattgactat ctgcaggatg
    60961 ctttcacaaa gcttggtcgt aacccgaacg acatcgagct gtatatgttc gcccaggcga
    61021 actccgagca ctgccgccac aagattttta acgccgattg ggttatcgac ggtgagcaac
    61081 agccgaaatc gctgtttaag atgatcaaaa acaccttcga aaccacgcca gattacgttc
    61141 tctctgctta taaagataac gccgccgtaa tggaaggttc tgaagtgggc cgctactttg
    61201 ctgaccacga aacgggccgc tacgatttcc atcaggaacc ggcgcatatt ctgatgaaag
    61261 tcgaaactca caaccacccg acggcgattt ctccgtggcc gggcgcggcg accggttccg
    61321 gcggtgaaat ccgcgatgaa ggtgccaccg ggcgcggcgc gaagccgaaa gcgggtttgg
    61381 ttggtttctc cgtttccaac ctgcgaattc ctggcttcga acagccgtgg gaagaagatt
    61441 tcggtaagcc tgagcgcatt gtcaccgcgc tggacatcat gaccgaaggc ccgctgggcg
    61501 gtgcggcgtt taacaacgaa tttggtcgtc cggcactgaa cggctacttc cgtacttatg
    61561 aagaaaaagt gaacagccac aacggcgaag agctgcgcgg ttatcacaaa ccgatcatgc
    61621 tggcgggcgg gatcggcaac attcgtgccg atcacgtaca aaaaggcgag atcaacgttg
    61681 gtgcgaaact ggtcgttctc ggcggcccgg cgatgaacat cggtcttggc ggcggcgcgg
    61741 cgtcttctat ggcgtctggt cagtctgatg ccgacctcga ctttgcttct gttcagcgcg
    61801 acaacccgga gatggagcgt cgctgccagg aagtgatcga ccgttgctgg cagcttggtg
    61861 atgctaaccc aattctgttt atccacgacg tcggcgcagg cggtctttct aacgcgatgc
    61921 cggaactggt gagcgacggc ggacgcggcg gtaaattcga actgcgcgat attctgaacg
    61981 acgaaccggg catgagcccg ctggaaatct ggtgtaacga atcccaggaa cgctacgtgc
    62041 tggcggttgc tgccgatcag ttaccgctgt ttgacgaact gtgtaagcgt gaacgcgcgc
    62101 cctacgcggt gattggtgaa gcgaccgaag aactgcatct ttctctgcac gatcgtcatt
    62161 ttgataatca gccgatcgat ctgccgctgg acgtcctgct tggtaaaacg ccgaagatga
    62221 cccgcgatgt acaaacgctg aaagcgaaag gcgacgcgct ggcccgtgaa gggatcacca
    62281 ttgcagatgc ggtgaaacgt gtgctgcatc tgccgactgt ggcggaaaaa accttcctgg
    62341 tgaccattgg cgaccgcagc gtaaccggca tggtagcgcg cgatcagatg gtggggccgt
    62401 ggcaggtgcc ggtcgctaac tgcgcggtca ctaccgccag cctcgacagc tactacggtg
    62461 aagcgatggc gattggcgag cgtgcgccgg ttgcgctgct ggatttcgcc gcctctgccc
    62521 gtctggcggt cggtgaagcg ttaaccaata ttgccgcaac acagattggt gatatcaaac
    62581 gcattaaact ttccgccaac tggatggcgg cggcaggcca ccctggtgaa gatgcgggcc
    62641 tgtatgaggc cgttaaagcc gtgggtgaag agctttgtcc ggcgctgggt cttacgatcc
    62701 cggtgggtaa agactctatg tcgatgaaaa cccgctggca ggaaggtaac gaagagcgcg
    62761 aaatgacgtc gccgctgtcg ctggtgattt ccgcatttgc ccgcgtggaa gatgtacgtc
    62821 acaccatcac gccgcagctt tctaccgaag ataacgcact gctgctgatt gatttgggca
    62881 aaggcaataa cgcgctgggc gcaacggcgc tggcgcaggt ttatcgtcag cttggcgaca
    62941 aaccggcaga tgtacgcgac gtcgcgcaac tgaaaggctt ctatgacgcg attcaggcgc
    63001 tggttgcaca gcgtaagctg ctggcgtatc acgaccgctc tgatggcggt ctgctggtaa
    63061 cgctggcgga aatggccttt gctggtcatt gtggcattga cgcggatatc gccactctgg
    63121 gtgacgatcg cctggcggcg ttgtttaacg aagaactggg tgcggtgatt caggttcgtg
    63181 ccgctgaccg tgaagcggtc gagtccgtac tggcacagca tgggcttgct gattgtgtcc
    63241 attatgttgg gcaggcggtt tccggtgacc gttttgtgat taccgccaac gggcagactg
    63301 tattcagcga aagccgcact acgttgcgtg tctggtgggc agaaactacc tggcagatgc
    63361 agcgcctgcg tgacaacccg gagtgtgctg accaggagca ccaggcgaaa tcaaacgacg
    63421 ccgatccggg cctgaatgta aaactgtcgt tcgatatcaa cgaagatgtg gcagcaccgt
    63481 ttattgccac tggcgcgcgc ccgaaagttg ccgtactgcg tgagcagggc gtgaactcgc
    63541 atgttgaaat ggcggcagct ttccaccgtg caggctttga tgctatcgac gtgcatatga
    63601 gtgacctgct ggctggacgc acgggcctgg aagatttcca cgccctggtc gcgtgcggtg
    63661 gtttctccta cggtgatgtg ctgggtgccg gtgaaggttg ggcgaagtca atcctgttca
    63721 atgaccgtgt acgcgatgag tttgcaacct tcttccaccg tccgcaaacg ctggcgctgg
    63781 gggtatgtaa cggttgccag atgatgtcta atctgcgtga actgatccca ggtagtgagt
    63841 tgtggccacg ttttgtgcgc aatacctccg atcgctttga agcgcgtttc agcctggttg
    63901 aagtgaccca aagcccgtcg ctgctgttgc aggggatggt gggctcgcaa atgccgattg
    63961 ctgtctctca cggtgaaggg cgcgtggaag tgcgtgatgc ggcgcatctg gcggcactgg
    64021 aaagcaaagg gctggtggca ctgcgctatg tcgataactt cggcaaagtc actgaaacct
    64081 acccggctaa cccgaacggt tctccgaacg gtattacggc agtcacgact gaaagtggtc
    64141 gagtcaccat tatgatgccg cacccggaac gtgttttccg tactgtcagc aactcctggc
    64201 atccggaaaa ctggggcgag gatggcccat ggatgcgcat tttccgcaat gcgcgtaagc
    64261 agttggggta agtcatcagt tcattggttt tgcagcccgg atgcggcatt tacgccacat
    64321 ccggcattaa gcaccaacca ctatcataag ctcccaactc cgggagcttt tttgtgtctg
    64381 taaatcacga caatgggtgg tttaccgtgt cgctttctgg cgacacttaa atgattgatt
    64441 ttattgtaat ctaataagtt tatcttaagt tgtctctttt tagcgacaca gtggctgatt
    64501 tacattcaaa tagcgaagaa gccataaatc cctcaaagca acgtataatc agtacgttac
    64561 caaactattt tctttattgg cacagttact gcataatagt aagcagtggc tcattcaccg
    64621 acttatgtca gccccttcgg gacgtgctac ataaaattcg aatgacgcac aacaaggtgc
    64681 ctgccgtcca acttctgata tcagcgtagc tatatcaacc atcgggcgaa acgtcgagtt
    64741 aggcaccgcc ttattccata acaaagccgg gtaattcccg gctttgttgt atctgaactt
    64801 cccctcggtt agcatcaggc tattcgcgtc tgacgagagt aacaccttga aacgctggcc
    64861 tgtttttccc cgctcattac gacaactggt aatgctggca tttttgctaa ttctactacc
    64921 cctgttggtg ctggcatggc aagcctggca aagcctgaat gcgcttagcg atcaggcggc
    64981 gctggttaac cgcactacgc ttattgatgc ccggcgtagt gaagcgatga ccaacgcggc
    65041 gctggagatg gagcgtagct accgtcagta ttgcgtgctg gacgatccta cgcttgcgaa
    65101 ggtttatcaa agccagcgca agcgttacag cgaaatgctc gatgcccacg caggcgtact
    65161 gccagacgat aaactttacc aggcattgcg tcaggatttg aacaatctgg cccaacttca
    65221 gtgtaacaac agtggtcccg atgctgccgc cgccgcgcgt cttgaagcct ttgccagtgc
    65281 caataccgaa atggtgcagg ccacgcgcac agtggtgttc tctcgtgggc agcaacttca
    65341 gcgtgaaatc gccgaacgtg ggcaatattt tggttggcaa tcgctggtgc tatttctggt
    65401 gagtctggtg atggtgctac ttttcacgcg gatgattatc gggccggtga aaaatatcga
    65461 gcgcatgatc aaccgtctgg gggaagggcg ttctctgggc aatagcgttt cgttcagtgg
    65521 accgagcgag ttacgctcgg ttgggcaacg tattctttgg ttaagtgagc gcctgtcatg
    65581 gctggaatcc caacgccatc aatttttaag acatttatct catgaattaa aaacgccact
    65641 ggcgagtatg cgcgagggca ctgaattact ggctgaccag gttgtcgggc cgcttacgcc
    65701 agagcaaaaa gaggtggtga gcattcttga tagcagcagc cgcaatttgc aaaaactgat
    65761 cgaacaactg cttgattaca accgtaaaca ggcggacagt gcggtggaac tggagaatgt
    65821 tgaattagca ccgctggtgg agacagtggt ttctgctcat agcctgcccg cacgggctaa
    65881 aatgatgcat accgacgtcg atctcaaagc aacagcttgc ctggcggagc caatgctgct
    65941 gatgagcgta ctggataatc tttactccaa tgcggtgcac tacggggctg aatccggtaa
    66001 catttgcctt cgcagcagtt cacatggtgc gcgggtttat attgatgtca tcaatacagg
    66061 cacgcccatt ccgcaagagg aacgcgccat gatcttcgaa cccttttttc agggaagcca
    66121 ccagcgaaaa ggggcggtga agggcagcgg tctgggatta agcattgcca gggattgtat
    66181 tcgccgtatg caaggggaac tgtatctggt cgacgagagc gggcaagacg tttgtttccg
    66241 cattgaatta ccgtcgtcga aaaacacgaa ataatgctaa cagcctatcc tataggctat
    66301 tttatttgtc agttcggccc cgggcagtgc tcgaaatctt cacgtacgat atgtacgctc
    66361 cggtttctcc gcgctgtcca tgtccagact gtctgcaaca attacgccca ctgggatagg
    66421 ctttaagtct ggtgaatatg cgacacattt ttcaacgatt attgccccga cggctgtggc
    66481 tggcagggct gccatgtctg gcattgctgg gttgcgtgca gagtcacaat aagccagcca
    66541 ttgatacgct agcagaagaa aaaattccgg tttatcaact ggctgactat ctttctactg
    66601 aatgtagcga catctgggcg ctgcaaggta aatcaaccga aaccaatccg ctttactggc
    66661 tgcgggcgat ggattgtgct gatcgtttaa tgcctgcgca gtcacgtcag caagctcgcc
    66721 aatatgacga cggcagctgg caaaatacct ttaaacaagg gattttgctc gccgacgcca
    66781 aaattacgcc gtatgagcgc cgccagctgg tcgcgcgcat tgatgcgcta agtaccgaga
    66841 tcccggcaca ggttcgccct ctgtatcaac tctggcgcga cggtcaggcg ctgcaactgc
    66901 aactggcgga agagcgccaa cgctacggca agctccagca ggccagcgac agcgagctgg
    66961 atacattgcg ccagcaacat cacgttctac agcagcaact ggaactcacc acccgcaagc
    67021 tggaaaacct gactgatatt gaacgccagc tctcgacccg caaaccggct ggaaatttct
    67081 cgccggatac gccgcacgag ggtgaaaagc ccgcgccatc cacccatgag gtcactcctg
    67141 atgagccata aacctgcgca tttattattg gtcgatgacg atccgggatt gctgaaactg
    67201 cttggcctgc gcctgaccag cgaaggctac agtgtggtca cggcggaaag tggcgctgaa
    67261 ggattacggg tactgaatcg cgaaaaagta gatttagtta tcagcgacct gcggatggat
    67321 gaaatggacg gtatgcagct gtttgctgaa atccagaaag tgcagccggg aatgccggta
    67381 attattctta ctgcgcatgg ttctatcccc gatgccgttg ctgcaacaca gcagggcgtt
    67441 tttagtttcc tcaccaagcc tgtcgacaaa gacgcgctat atcaggcaat tgacgatgcg
    67501 ctggagcaat ccgcgccagc caccgatgaa cgctggcgcg aggcaattgt cacccgcagc
    67561 ccgctgatgc tgcgtttgct ggaacaggcg cggctggtgg cgcaatcaga cgtcagcgtg
    67621 ttgattaatg gccagagcgg caccgggaaa gagattttcg cccaggctat ccacaacgcc
    67681 agcccgcgta acagcaaacc atttattgct attaactgtg gcgcattgcc cgagcagttg
    67741 ctggagtcgg agctgtttgg tcatgcgcgt ggcgcgttta ctggcgctgt cagcaatcgc
    67801 gaaggtttat tccaggcggc ggaaggcggt acgctatttc tcgatgagat tggcgatatg
    67861 cccgcaccat tgcaggtcaa actgctgcgc gtgttgcagg agcgtaaagt gcgcccgctg
    67921 ggcagcaacc gcgatattga tatcgacgtg cggattattt ccgccactca ccgcgacctg
    67981 ccaaaagcga tggcgcgcgg ggaattccgt gaagatctct attaccgcct caacgttgtc
    68041 agcctgaaaa ttccggctct ggcagagcgc acagaagaca ttccgcttct ggcaaatcac
    68101 ctgttgcgcc aggcggcaga gcgacataaa ccgtttgtcc gcgcgttctc cactgatgcg
    68161 atgaaacgcc tgatgaccgc tagctggccg ggtaatgtgc gccagttggt caacgtgatt
    68221 gaacagtgcg tggcgctgac ctcatctccg gttattagcg atgcattagt agagcaggcg
    68281 ctggagggtg aaaacacggc gctgccaacc tttgttgagg cacgtaatca gtttgaactc
    68341 aactatttgc gtaagctgct gcaaattacc aaaggcaacg tcacccacgc ggcgagaatg
    68401 gcggggcgca accggacaga attttataaa ctgctttccc gacacgagct ggatgcaaac
    68461 gatttcaagg aatgaattgg cgttatgtgt tacgtttagc agatcaaaag acaggcgacc
    68521 ttttcaagga atagcatgaa aaagattgat gcgattataa aacccttcaa gctggacgat
    68581 gtccgcgaag cactggccga agtcggtatt accggcatga cggtgaccga agtgaaaggc
    68641 tttggtcgcc agaaaggcca taccgagctg taccgcggcg cggagtatat ggtggatttt
    68701 ctgccgaaag tgaaaattga gattgtcgta ccggacgaca ttgtcgatac ctgtgtcgat
    68761 accattattc gcacggcgca aaccggcaaa atcggtgacg gtaaaatctt cgtctttgac
    68821 gtggcacggg tcattcgcat ccgtaccggt gaagaggacg acgcggcaat ttaaaccgcg
    68881 tcaagggttg ccggatgttt ccatccggca acatcaaatc acagcacctt atgcgggcca
    68941 aagcattcgt aatgaatgtt ttcctgcttc acgcccagat ccactaactg tttcgcggca
    69001 aactgcatga agccaaccgg gccgcagaga tagaactgca ttgtcggatc gctgaacgca
    69061 ccttcctgtt tgctcaaatc catcagacct tcgctatcaa actgaccttt agcgcgatcg
    69121 gcttcgctcg gctgacgata ccaggtgtgc gcggtaaagc gcggcagtga ctgccccagt
    69181 tccttaactt catcggcaaa ggcgtgaaca tcgccatttt ctgccgcatg gaaccagttc
    69241 acttgtgctg tgtggcctgc ttttgccagc gtgtcgagca ttgccagcat tggcgtttga
    69301 ccaacaccgg cagagattaa cgtcactggt gtgtcatctg cgacagccat aaagaaatca
    69361 cctgccggag cgaccagttt cacgacatcg ccaacattgg cgtgattgtg caaccagttg
    69421 gatacctgcc caccctcttc gcgtttcacc gcaatacgat agcctttgcc atccggtttg
    69481 cgagtcaaag agtactgacg aatttcctga tgcgggaaac cttccggctt cagccagacg
    69541 ccgagatatt gccccggacg gtattctgcc actgcgccac cgtcgaccgg ctccagttcg
    69601 aagctggtga taagcgcgct gcgcggtgtt ttagccacaa tgcggaaatc gcgagtacct
    69661 tcccaaccac cggctttgct ggcgttttcg ttatagattt ccgcctcgcg attgataaat
    69721 acattagcca gtacaccata ggctttaccc cacgcgtcca gcacttcctg ccccgggctg
    69781 aacatttcgt ccagcgttgc caacaggtgt tcaccgacga tgttgtactg ttccggtttg
    69841 atctggaagc tggtgtgctt ctgcgcgatt ttttctaccg ctggcagcag cgcaggcagg
    69901 ttttcaatat tactggcgta ggcggcaata gcgttaaaca gggcttcacg ttgatcgcca
    69961 ttacgctggt tactcatgtt aaaaatttct ttgagttctg ggttatgagt aaacatacgg
    70021 tcgtagaaat gggcggttaa ctttggcccc gtttccacca gtaaagggat ggtggctttt
    70081 actgtagcga tggtttgagc gtcaagcata tggtcttcct tttttttgca tcttaattga
    70141 tgtatctcaa atgcatctta taaaaaatag ccctgcaatg taaatggttc tttggtgttt
    70201 ttcagaaaga atgtgatgaa gtgaaaaatt tgcatcacaa acctgaaaag aaatccgttt
    70261 ccggttgcaa gctctttatt ctccaaagcc ttgcgtagcc tgaaggtaat cgtttgcgta
    70321 aattcctttg tcaagacctg ttatcgcaca atgattcggt tatactgttc gccgttgtcc
    70381 aacaggaccg cctataaagg ccaaaaattt tattgttagc tgagtcagga gatgcggatg
    70441 ttaaagcgtg aaatgaacat tgccgattat gatgccgaac tgtggcaggc tatggagcag
    70501 gaaaaagtac gtcaggaaga gcacatcgaa ctgatcgcct ccgaaaacta caccagcccg
    70561 cgcgtaatgc aggcgcaggg ttctcagctg accaacaaat atgctgaagg ttatccgggc
    70621 aaacgctact acggcggttg cgagtatgtt gatatcgttg aacaactggc gatcgatcgt
    70681 gcgaaagaac tgttcggcgc tgactacgct aacgtccagc cgcactccgg ctcccaggct
    70741 aactttgcgg tctacaccgc gctgctggaa ccaggtgata ccgttctggg tatgaacctg
    70801 gcgcatggcg gtcacctgac tcacggttct ccggttaact tctccggtaa actgtacaac
    70861 atcgttcctt acggtatcga tgctaccggt catatcgact acgccgatct ggaaaaacaa
    70921 gccaaagaac acaagccgaa aatgattatc ggtggtttct ctgcatattc cggcgtggtg
    70981 gactgggcga aaatgcgtga aatcgctgac agcatcggtg cttacctgtt cgttgatatg
    71041 gcgcacgttg cgggcctggt tgctgctggc gtctacccga acccggttcc tcatgctcac
    71101 gttgttacta ccaccactca caaaaccctg gcgggtccgc gcggcggtct gatcctggcg
    71161 aaaggtggta gcgaagagct gtacaaaaaa ctgaactctg ccgttttccc tggtggtcag
    71221 ggcggtccgt tgatgcatgt aatcgctggt aaagcggttg ctctgaaaga agcgatggag
    71281 cctgagttca aaacttacca gcagcaggtc gctaaaaacg ctaaagcgat ggtagaagtg
    71341 ttcctggaac gcggctacaa agtggtttcc ggcggcactg ataaccacct gttcctggtt
    71401 gatctggttg ataaaaacct gaccggtaaa gaagcagacg ccgctctggg ccgtgctaac
    71461 atcaccgtca acaaaaacag cgtaccgaac gatccgaaga gcccgtttgt gacctccggt
    71521 attcgcgtgg gtactccggc aattacgcgt cgcggcttca aagaagcaga agcgaaagaa
    71581 ctggctggct ggatgtgtga cgtgctggac agcatcaatg atgaagccgt tatcgagcgc
    71641 atcaaaggta aagttctcga catctgcgca cgttacccgg tttacgcata agcgaaacgg
    71701 ttaatctgtt gaaaaaaggc ttccttcggg aagcctttct acgttatcgc gccatcaaat
    71761 ctgtcgtaac tgcgcctcaa catacaaata gccaattccc agcacctgtt gtgcgcggct
    71821 taattgcccg aagccaattt gcgtcgcttt cacgctcggt ccttcgtcgc ggtcgaacgg
    71881 gttaaatcct gtctggcgaa taatagtatt aagccagttc tcaccaaagg cacaactgcg
    71941 accgtacaac caaatctgat taatattgag gatgttgagg aagttataca gactcaaacc
    72001 aatggcattg gcactgcggt caacccagct ggtgatccac ggttctccac tctgccaggc
    72061 agcgattaac tgcgcggttg tcagtttttc aggatcaagc tgagtattaa ccggttgtga
    72121 ttttagccat acccgcgcct gtttttttaa agcacttaac gaggcgacgg tttccaggca
    72181 gccgtaacgt ccacagtcgc agacgacgcc atccggatta acgatggtgt gaccaatctg
    72241 tccgctacca tacaaactgc cgcgataaat ttgctcgtta atcacgaacg acgagccaat
    72301 gccgtaatca acgttgatca cgcagaaatc ccgttcctgc gaattatttt gccatttctc
    72361 cgccagcgcc agcatcacgc agtcattatc gaccatcacc cgaatgccga gcttctcttc
    72421 cagcagatat ttcacctcca ccggcgttgt ccacggcgct tgcggcatgg tttgcgacac
    72481 gccggtcacc ggatcaacct gaccgtggat tgccagcgcc agattgatgg tgcggtccgg
    72541 ccacaattta cggtggcgat gccagcattt ttcgatttcg gacagcagcg cctgcggcgt
    72601 cggtgcatca atctgtaaat actcaaactc gcctttcgga cttaaacaag cattagcaac
    72661 ctgacactca atactggtgg gcgtcacgtt caggcacagc gtccagtcac cttccggcgc
    72721 aatcagccat gtaccgctac tatgcccgcg cgtctggctt tcatcgtcga tattcaccac
    72781 ccgtttttcg ctttccagtt cctgcaaaat attactgact gccggaatcg aaatttgcgc
    72841 cagccgggcc agcgttgatt tattggcgcg cttttgccgg tacagcagtt ccagaatcac
    72901 gcatttgtta tggtggcgaa tctgttgatt gttaatgcag gctctcatct tcactaaacc
    72961 ttgtttagtt aattgcgtga ttattccgcg aaaacattgc cagattgttc aatacactgc
    73021 cacaaatctt ttaattcagt atgtcttgtt aatattgagg gcaccatgac tccagtaaaa
    73081 gtgtggcaag agcgcgttga gatcccgacc tatgaaaccg ggccgcagga tatacatccc
    73141 atgttcctgg aaaatcgcgt ttatcaggga tcgtccggcg cggtttatcc ctacggcgtg
    73201 accgatacgc tgagcgagca gaaaaccctg aaatcctggc aggcggtgtg gctggaaaac
    73261 gactacatca aagtgatgat cctgccggaa ctgggcggtc gtgtgcatcg cgcatgggat
    73321 aaagtgaaac aacgcgattt tgtctatcac aatgaagtca ttaaacctgc gctggtgggg
    73381 ctgctgggac cgtggatctc tggcgggatt gagtttaact ggccgcaaca ccatcgcccg
    73441 accaccttta tgcccgttga tttcaccctc gaagcccatg aagacggcgc acagacggtg
    73501 tgggtcggcg aaacggagcc gatgcacggt ttacaggtga tgacaggttt cacactgcgt
    73561 cctgaacggg cggcgttgga aattgccagc cgcgtctata acggcaacgc cacgccgcgt
    73621 catttcttgt ggtgggccaa cccggcagtg aaaggggggg aagggcatca gagcgtcttc
    73681 ccgccggatg taacggcggt gtttgatcac ggcaaacggg ccgtctccgc tttccccatc
    73741 gccaccggca cttactacaa agtggactac tccgccggag tggacatttc tcgctataaa
    73801 aatgtgcccg ttccaacctc atatatggct gaaaaatcac agtacgattt tgttggcgcg
    73861 tggtgtcacg atgaagatgg cggtttgcta cacgttgcca accaccatat cgcgccaggt
    73921 aaaaaacagt ggagctgggg acacagtgaa ttcggccagg cgtgggacaa gagtctgacc
    73981 gacaataacg gcccgtatat cgaactgatg accggtattt ttgccgataa ccagcctgat
    74041 tttacctggc ttgatgctta cgaagagaag cgttttgagc agtatttcct gccttatcat
    74101 tctttgggca tggtgcaaaa tgcctcccgc gatgcggtga taaaactcca gcgtagtgag
    74161 cgggggattg agtgggggct gtatgccatc tctccgttga acggataccg cctggcgatc
    74221 cgcgaaatcg gcaaatgcaa cgcgttactt gatgatgccg tggcactgat gcctgcgacc
    74281 gccatccagg gcgtgttgca cggtatcaat cctgaaaggc tgaccattga gctctccgat
    74341 gccgacggca atattgtcct gagttatcag gaacatcagc cgcaagagtt gccgttgccg
    74401 gacgtcgcca aagcgccact gtcagcacaa gacattacca gtacagatga agcctggttt
    74461 atcggtcagc atctggagca atatcatcac gccagccgtt caccgttcga ttactacctg
    74521 cgcggcgtgg cgctggatcc gctggattac cgctgtaacc tggcgctggc gatgctggaa
    74581 tataaccgtg ccgatttccc gcaagcggtg gcgtatgcca ctcaggctct gaaacgcgca
    74641 catgcgctga acaaaaatcc gcagtgcgga caggcgagtt tgattcgcgc cagtgcttac
    74701 gaacgtcagg gacaatatca acaagccgaa gaggatttct ggcgggcggt ctggagcggc
    74761 aacagcaaag ccggtggcta ttatggcctg gcacgactgg ctgcgcgtaa tggtaacttc
    74821 gacgcgggtc tggatttttg ccaacaaagt cttcgcgcct gcccaaccaa tcaggaagtg
    74881 ctttgcctgc ataacctgct gctggtgtta agtggtcttc aggacaacgc gcgtttgcag
    74941 cgcgagaaac tgctgcgcga ttatccgctg aacgccactc tgtggtggct gaactggttc
    75001 gatggtcgta gcgaatcagc cctcgcgcag tggcgcggtc tgtgtcaggg acgcgacgtt
    75061 aacgctctga tgaccgccgg gcaactgatt aactggggaa tgcccaccct ggcggcagag
    75121 atgctgaacg cactggactg ccagcgcacg ctgccgcttt acctgcaagc cagcttgctg
    75181 ccgaaagccg aacgtggcga actggtcgca aaagccattg atgtcttccc gcagtttgtc
    75241 cgtttcccga atacgttgga agaagtggcg gcgctggaga gtattgaaga gtgctggttt
    75301 gctcgccatt tactggcctg cttctactac aacaaacgta gctacaacaa agccattgcc
    75361 ttatggcaac gttgcgtaga gatgtcgccg gagtttgccg acggctggcg cgggttagcg
    75421 atccatgcgt ggaataagca acacgattat gagctggccg cgcgttatct tgataatgct
    75481 tatcagcttg cgccgcagga tgcacgtctg cttttcgaac gggatttgct tgataagtta
    75541 agtggagcca caccggagaa acgactggcg cgtctggaaa ataatcagga aattgcgctg
    75601 aaacgcgacg acatgaccgc agaactgctc aatttgtggc atctcacggg tcaggcagac
    75661 aaagcggcgg acattctcgc cacgcgtaaa ttccacccgt gggaaggcgg ggaagggaag
    75721 gtcaccagtc agtttatcct caaccagtta ttacgcgcct ggcagcatct tgatgccaga
    75781 gagccgcagc aggccagcga actgcttcat gccgcgctgc attatccgga gaatttaagc
    75841 gaaggccgtt taccggggca aactgataac gacatctggt tctggcaggc gatatgcgcc
    75901 aacgcgcagg gcgatgaaac tgaagcgacg cgttgtttac gtctggcggc gaccggcgat
    75961 cgcaccatta acatccacag ttattacaac gatcagccgg ttgattatct cttctggcaa
    76021 ggaatggcgc tgcgactgct gggtgaacag caaaccgcac agcaactgtt tagtgaaatg
    76081 aaacagtggg cgcaagagat ggcgaaaacc agtattgagg cggatttctt tgctgtttca
    76141 caacctgacc tgttatcgct gtatggcgat ttacaacagc agcataaaga aaaatgcctg
    76201 atggtggcga tgctggcgtc cgcgggactc ggggaggttg cgcaatatga gtctgctcgc
    76261 gctgaattga cggcgattaa tccggcctgg ccgaaagcgg cattattcac caccgtgatg
    76321 ccttttattt ttaactacgt tcactaaacc ctattaactg taatatttga gcggcacgcg
    76381 ctgccgctca tcacaaaata attagaataa gccgttgtta ataaaaaccc tacaggaggc
    76441 actatgccta aaaaaatgag aacaacccgt aatctattat taatggctac gctattaggt
    76501 agcgcactat ttgccagggc tgcggataaa gaaatgacca ttggtgcaat ataccttgat
    76561 acccagggat attacgctgg agtgcgtcag ggcgttcagg atgcggcaaa agattcttca
    76621 gtacaggtac agttaattga aactaacgcc cagggtgata tttcgaaaga aagtaccttt
    76681 gttgataccc tcgtggcgcg taatgtcgat gccattattt tatcggcagt gtctgaaaat
    76741 ggcagtagcc gtaccgttcg tcgcgccagt gaagcgggta ttccggtgat ttgctacaac
    76801 acctgtatta atcaaaaggg ggtcgataaa tatgtctcgg cgtatctggt cggcgatcca
    76861 ctggaatttg gtaaaaaact gggtaacgct gccgccgatt attttattgc caataaaatt
    76921 gaccagccga aaattgccgt catcaattgc gaagcctttg aagtttgtgt gcagcgacgt
    76981 aaaggatttg aagaagtatt aaaatcccgc gttcccggcg cgcaaattgt cgctaatcag
    77041 gaagggactg ttttagataa agcgatttcc gttggtgaaa aactgattat ctccacgccg
    77101 gatctcaacg ccattatggg ggagtcgggc ggtgcgacac tcggcgcggt aaaagcggta
    77161 cgtaatcaaa atcaggccgg aaaaattgct gttttcggtt cggatatgac aaccgaaatt
    77221 gctcaggagc tggaaaacaa tcaggtgctg aaagcggtag tggatatttc cggtaagaaa
    77281 atgggcaatg ctgttttcgc gcaaacattg aaggttatca ataaacaagc cgacggtgaa
    77341 aaagtgattc aggtgccaat cgatctctat accaaaacgg aagatggtaa acagtggctg
    77401 gcaacgcacg ttgatggtct gccctaatcg ctgattcgca gaggtcccta tgttcacggc
    77461 aacagaggca gtcccggtag caaaagtggt ggcaggaaat aagcgttacc ccggcgtcgt
    77521 tgcgctggat aacgttaact tcacgctcaa taaaggcgaa gttcgtgcgc tgttaggtaa
    77581 aaacggcgcg ggcaaatcga ctctcattcg aatgcttacc ggcagcgaac gcccggatag
    77641 cggtgatatc tggattggcg agacgcgact ggaaggtgac gaagctacgt tgactcgccg
    77701 tgccgctgaa ctgggggttc gtgcggttta tcaggaatta agtctggtgg aagggctgac
    77761 ggtggcagaa aacctctgcc ttggtcaatg gcctcgccgc aacggcatga ttgattacct
    77821 gcaaatggcg caggatgccc aacgttgctt acaggcgctg ggcgttgacg ttagtcctga
    77881 acaacttgtt tcaacgctaa gcccggcgca aaagcaactg gtggaaattg cacgggtgat
    77941 gaagggcgag ccgcgcgtgg tcattcttga tgaacccacc agctcgcttg ccagtgcaga
    78001 agttgaactg gtgatcagtg cggtgaaaaa gatgtcggca ctgggcgtgg cggtgattta
    78061 tgtcagccac cggatggaag aaatccgccg cattgcctcc tgtgccaccg ttatgcgcga
    78121 tggtcaggtg gcgggcgatg tgatgctcga aaacacctcc acgcatcata ttgtgtcgct
    78181 aatgctcggg cgtgatcacg ttgatattgc gccggttgcc cctcaggaaa ttgtcgatca
    78241 ggccgtactg gaagtccgtg cgttacgcca taagcccaag ctggaggata tcagctttac
    78301 gctacgtcgt ggcgaagtgc ttggcattgc tggcctgctg ggggcagggc gcagtgaatt
    78361 gctgaaggcg attgttgggc tggagacgta tgaacagggc gaaattgtta tcaacggcga
    78421 gaaaatcatg cgccccgatt acggcgacat gctgaaacgc ggcattggct atacgccaga
    78481 aaaccgcaaa gaagcgggga tcattccctg gctgggcgtt gacgaaaata cagtgctgac
    78541 caatcggcaa aaaatcagca ccaacggtgt gctgcaatgg tccaccatcc gccgcctgac
    78601 cgaagaggtg atgcagcgga tgacggtcaa ggctgccagt agcgaaacac ccatcggcac
    78661 gctttctggt ggcaatcagc aaaaagtggt gatcggtcgt tgggtctatg ccgccagcca
    78721 gattttgttg ctcgacgagc caacgcgcgg cgtcgatatc gaagccaaac agcagattta
    78781 ccgtattgtc cgtgagctgg ctgccgaagg aaaaagcgtg gtgtttatct ccagtgaagt
    78841 ggaggagttg ccgctggtgt gtgaccgcat cctgttatta cagcacggca cgttctcgca
    78901 ggagtttcac tcgccggtca atgtggatga gctgatgtcc gccattctgt ctgtgcactg
    78961 atga
//
LOCUS       DAEVZA010000005        77424 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEVZA010000005 DAEVZA010000000
VERSION     DAEVZA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.005"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1035
                     /locus_tag="LAF92_000351"
     CDS             <1..1035
                     /locus_tag="LAF92_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBI9789414.1"
                     /translation="QAEFINNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITT
                     TPGVYDSTGLLNLKELPGHLGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDR
                     DIADNIATILRDQGVDIILNAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATA
                     SLHPENAGIAVNERGAIVVDKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELL
                     GEGRRSTDDRKNVPYSVFMTPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMND
                     TRGVLKAIVDNKTQRILGASLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSE
                     SLNDLFSLVK"
     gene            1144..1380
                     /locus_tag="LAF92_000352"
     CDS             1144..1380
                     /locus_tag="LAF92_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI9789415.1"
                     /translation="MFKKSVLFATLLSGVMAFSANADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            1392..1985
                     /gene="rclC"
                     /locus_tag="LAF92_000353"
     CDS             1392..1985
                     /gene="rclC"
                     /locus_tag="LAF92_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HBI9789416.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(2145..3014)
                     /locus_tag="LAF92_000354"
     CDS             complement(2145..3014)
                     /locus_tag="LAF92_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBI9789417.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            3263..4120
                     /locus_tag="LAF92_000355"
     CDS             3263..4120
                     /locus_tag="LAF92_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI9789418.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKPTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(4241..8494)
                     /gene="fdeC"
                     /locus_tag="LAF92_000356"
     CDS             complement(4241..8494)
                     /gene="fdeC"
                     /locus_tag="LAF92_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HBI9789419.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAANTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLNLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADTATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAAIYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            9610..9711
                     /locus_tag="LAF92_000357"
     CDS             9610..9711
                     /locus_tag="LAF92_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789420.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            10074..10337
                     /locus_tag="LAF92_000358"
     CDS             10074..10337
                     /locus_tag="LAF92_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBI9789421.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            10337..10477
                     /gene="ykgO"
                     /locus_tag="LAF92_000359"
     CDS             10337..10477
                     /gene="ykgO"
                     /locus_tag="LAF92_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HBI9789422.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(10512..10739)
                     /locus_tag="LAF92_000360"
     CDS             complement(10512..10739)
                     /locus_tag="LAF92_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789423.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            11562..12104
                     /gene="ecpR"
                     /locus_tag="LAF92_000361"
                     /gene_synonym="matA"
     CDS             11562..12104
                     /gene="ecpR"
                     /locus_tag="LAF92_000361"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HBI9789424.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            12179..12766
                     /gene="ecpA"
                     /locus_tag="LAF92_000362"
                     /gene_synonym="matB"
     CDS             12179..12766
                     /gene="ecpA"
                     /locus_tag="LAF92_000362"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HBI9789425.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            12824..13492
                     /gene="ecpB"
                     /locus_tag="LAF92_000363"
                     /gene_synonym="yagY"
     CDS             12824..13492
                     /gene="ecpB"
                     /locus_tag="LAF92_000363"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HBI9789426.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGYLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            13518..16043
                     /gene="ecpC"
                     /locus_tag="LAF92_000364"
                     /gene_synonym="yagX"
     CDS             13518..16043
                     /gene="ecpC"
                     /locus_tag="LAF92_000364"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HBI9789427.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            16033..17676
                     /gene="ecpD"
                     /locus_tag="LAF92_000365"
     CDS             16033..17676
                     /gene="ecpD"
                     /locus_tag="LAF92_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HBI9789428.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            17645..18355
                     /gene="ecpE"
                     /locus_tag="LAF92_000366"
     CDS             17645..18355
                     /gene="ecpE"
                     /locus_tag="LAF92_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HBI9789429.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            18668..18997
                     /locus_tag="LAF92_000367"
     CDS             18668..18997
                     /locus_tag="LAF92_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBI9789430.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(19245..19859)
                     /locus_tag="LAF92_000368"
     CDS             complement(19245..19859)
                     /locus_tag="LAF92_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HBI9789431.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            20277..20966
                     /gene="paoA"
                     /locus_tag="LAF92_000369"
     CDS             20277..20966
                     /gene="paoA"
                     /locus_tag="LAF92_000369"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HBI9789432.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            20963..21919
                     /gene="paoB"
                     /locus_tag="LAF92_000370"
     CDS             20963..21919
                     /gene="paoB"
                     /locus_tag="LAF92_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HBI9789433.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            21916..24114
                     /gene="paoC"
                     /locus_tag="LAF92_000371"
     CDS             21916..24114
                     /gene="paoC"
                     /locus_tag="LAF92_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HBI9789434.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            24124..25080
                     /gene="paoD"
                     /locus_tag="LAF92_000372"
     CDS             24124..25080
                     /gene="paoD"
                     /locus_tag="LAF92_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HBI9789435.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNWELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            25131..25469
                     /locus_tag="LAF92_000373"
                     /pseudo
     CDS             25131..25469
                     /locus_tag="LAF92_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(25596..25671)
                     /locus_tag="LAF92_000374"
     tRNA            complement(25596..25671)
                     /locus_tag="LAF92_000374"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25636..25638),aa:Thr,seq:cgt)
     gene            complement(25786..27039)
                     /gene="proA"
                     /locus_tag="LAF92_000375"
     CDS             complement(25786..27039)
                     /gene="proA"
                     /locus_tag="LAF92_000375"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBI9789436.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(27051..28154)
                     /gene="proB"
                     /locus_tag="LAF92_000376"
     CDS             complement(27051..28154)
                     /gene="proB"
                     /locus_tag="LAF92_000376"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBI9789437.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            28442..29497
                     /gene="phoE"
                     /locus_tag="LAF92_000377"
     CDS             28442..29497
                     /gene="phoE"
                     /locus_tag="LAF92_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBI9789438.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(29536..29937)
                     /gene="crl"
                     /locus_tag="LAF92_000378"
     CDS             complement(29536..29937)
                     /gene="crl"
                     /locus_tag="LAF92_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBI9789439.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(29995..31239)
                     /gene="frsA"
                     /locus_tag="LAF92_000379"
     CDS             complement(29995..31239)
                     /gene="frsA"
                     /locus_tag="LAF92_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBI9789440.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(31331..31789)
                     /gene="gpt"
                     /locus_tag="LAF92_000380"
     CDS             complement(31331..31789)
                     /gene="gpt"
                     /locus_tag="LAF92_000380"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBI9789441.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            32050..33507
                     /gene="pepD"
                     /locus_tag="LAF92_000381"
     CDS             32050..33507
                     /gene="pepD"
                     /locus_tag="LAF92_000381"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBI9789442.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(33564..34178)
                     /locus_tag="LAF92_000382"
     CDS             complement(33564..34178)
                     /locus_tag="LAF92_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBI9789443.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(34175..35314)
                     /locus_tag="LAF92_000383"
     CDS             complement(34175..35314)
                     /locus_tag="LAF92_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBI9789444.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTVTQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(35560..36012)
                     /locus_tag="LAF92_000384"
     CDS             complement(35560..36012)
                     /locus_tag="LAF92_000384"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI9789445.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQY"
     gene            complement(36009..37064)
                     /gene="dinB"
                     /locus_tag="LAF92_000385"
     CDS             complement(36009..37064)
                     /gene="dinB"
                     /locus_tag="LAF92_000385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBI9789446.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(37135..38058)
                     /gene="lafU"
                     /locus_tag="LAF92_000386"
     CDS             complement(37135..38058)
                     /gene="lafU"
                     /locus_tag="LAF92_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBI9789447.1"
                     /translation="MRKTGNRRDRGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(38061..38924)
                     /gene="motA"
                     /locus_tag="LAF92_000387"
     CDS             complement(38061..38924)
                     /gene="motA"
                     /locus_tag="LAF92_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBI9789448.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(38937..39653)
                     /locus_tag="LAF92_000388"
     CDS             complement(38937..39653)
                     /locus_tag="LAF92_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBI9789449.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(39673..40140)
                     /locus_tag="LAF92_000389"
     CDS             complement(39673..40140)
                     /locus_tag="LAF92_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBI9789450.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(40148..41209)
                     /locus_tag="LAF92_000390"
     CDS             complement(40148..41209)
                     /locus_tag="LAF92_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBI9789451.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(41206..41517)
                     /locus_tag="LAF92_000391"
     CDS             complement(41206..41517)
                     /locus_tag="LAF92_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBI9789452.1"
                     /translation="MEKQRRQLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLCAVKARLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(41522..41914)
                     /gene="fliS"
                     /locus_tag="LAF92_000392"
     CDS             complement(41522..41914)
                     /gene="fliS"
                     /locus_tag="LAF92_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBI9789453.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(41937..43253)
                     /gene="fliD"
                     /locus_tag="LAF92_000393"
     CDS             complement(41937..43253)
                     /gene="fliD"
                     /locus_tag="LAF92_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBI9789454.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(43459..44373)
                     /gene="lafA"
                     /locus_tag="LAF92_000394"
     CDS             complement(43459..44373)
                     /gene="lafA"
                     /locus_tag="LAF92_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HBI9789455.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            44859..45701
                     /locus_tag="LAF92_000395"
     CDS             44859..45701
                     /locus_tag="LAF92_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBI9789456.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNPVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            complement(45756..46733)
                     /locus_tag="LAF92_000396"
     CDS             complement(45756..46733)
                     /locus_tag="LAF92_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HBI9789457.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQVHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESMNQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(46750..47679)
                     /gene="flgL"
                     /locus_tag="LAF92_000397"
     CDS             complement(46750..47679)
                     /gene="flgL"
                     /locus_tag="LAF92_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBI9789458.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQDPNADPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(47694..49070)
                     /gene="flgK"
                     /locus_tag="LAF92_000398"
     CDS             complement(47694..49070)
                     /gene="flgK"
                     /locus_tag="LAF92_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBI9789459.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(49374..49673)
                     /locus_tag="LAF92_000399"
     CDS             complement(49374..49673)
                     /locus_tag="LAF92_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HBI9789460.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHR"
     gene            complement(49673..50773)
                     /locus_tag="LAF92_000400"
     CDS             complement(49673..50773)
                     /locus_tag="LAF92_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBI9789461.1"
                     /translation="MQNWIKAAAIAVSLALPGVVLAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(50788..51453)
                     /gene="flgH"
                     /locus_tag="LAF92_000401"
     CDS             complement(50788..51453)
                     /gene="flgH"
                     /locus_tag="LAF92_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBI9789462.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(51725..52510)
                     /gene="flgG"
                     /locus_tag="LAF92_000402"
     CDS             complement(51725..52510)
                     /gene="flgG"
                     /locus_tag="LAF92_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBI9789463.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(52689..53426)
                     /locus_tag="LAF92_000403"
     CDS             complement(52689..53426)
                     /locus_tag="LAF92_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HBI9789464.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNAEGMLITADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(53426..54628)
                     /gene="flgE"
                     /locus_tag="LAF92_000404"
     CDS             complement(53426..54628)
                     /gene="flgE"
                     /locus_tag="LAF92_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlgE"
                     /protein_id="HBI9789465.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     AAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(54713..55426)
                     /locus_tag="LAF92_000405"
     CDS             complement(54713..55426)
                     /locus_tag="LAF92_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HBI9789466.1"
                     /translation="MNTLALNAQSPQPTATQESSNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSGQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPQGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(55426..55857)
                     /gene="flgC"
                     /locus_tag="LAF92_000406"
     CDS             complement(55426..55857)
                     /gene="flgC"
                     /locus_tag="LAF92_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBI9789467.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(55860..56195)
                     /gene="flgB"
                     /locus_tag="LAF92_000407"
     CDS             complement(55860..56195)
                     /gene="flgB"
                     /locus_tag="LAF92_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314272.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBI9789468.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAVVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            56277..57014
                     /gene="flgA"
                     /locus_tag="LAF92_000408"
     CDS             56277..57014
                     /gene="flgA"
                     /locus_tag="LAF92_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBI9789469.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDTPLTANDVELKPYNVSAQRRDVLMVLDDAI
                     GFSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGISASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            57095..57373
                     /gene="flgM"
                     /locus_tag="LAF92_000409"
     CDS             57095..57373
                     /gene="flgM"
                     /locus_tag="LAF92_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBI9789470.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTATTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            57386..57814
                     /locus_tag="LAF92_000410"
     CDS             57386..57814
                     /locus_tag="LAF92_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBI9789471.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRERLQRQRICMIRRASEEL
                     LAVNEEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            57941..58870
                     /locus_tag="LAF92_000411"
     CDS             57941..58870
                     /locus_tag="LAF92_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HBI9789472.1"
                     /translation="MSFIECYEPEYVRKFLARHPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSRASKMPGRDGDILARLTTLPLALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDVIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            59049..61493
                     /locus_tag="LAF92_000412"
     CDS             59049..61493
                     /locus_tag="LAF92_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBI9789473.1"
                     /translation="MPTPTIIKTLNTHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAACEDENGQRGRRLSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQLSNDEHSLEDVWQPLLRADKWLACYPSLCYQRPGFSDIEKKVTDN
                     ISHYFNKLPVSTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQAHSQRVKCLVSPYHTPALNGLSPVNIRAM
                     YGLAKETWNHADWNRFYQRILCYSHYSQRALEHFGNAKVVGNPRFDAWHNGTFDRALP
                     ENIQPDSCKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTSSRPQEAASL
                     ALARRHLKQLTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMTELLD
                     GEKSYSTPESADQRIREILPVAHEVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(61611..62006)
                     /locus_tag="LAF92_000413"
     CDS             complement(61611..62006)
                     /locus_tag="LAF92_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HBI9789474.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(62009..62443)
                     /gene="fliJ"
                     /locus_tag="LAF92_000414"
     CDS             complement(62009..62443)
                     /gene="fliJ"
                     /locus_tag="LAF92_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBI9789475.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTIELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(62446..63783)
                     /gene="fliI"
                     /locus_tag="LAF92_000415"
     CDS             complement(62446..63783)
                     /gene="fliI"
                     /locus_tag="LAF92_000415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBI9789476.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(63776..64486)
                     /locus_tag="LAF92_000416"
     CDS             complement(63776..64486)
                     /locus_tag="LAF92_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HBI9789477.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(64489..65499)
                     /locus_tag="LAF92_000417"
     CDS             complement(64489..65499)
                     /locus_tag="LAF92_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBI9789478.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(65477..67123)
                     /gene="fliF"
                     /locus_tag="LAF92_000418"
     CDS             complement(65477..67123)
                     /gene="fliF"
                     /locus_tag="LAF92_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBI9789479.1"
                     /translation="MNAQIKKLTQAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNGESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(67128..67469)
                     /locus_tag="LAF92_000419"
     CDS             complement(67128..67469)
                     /locus_tag="LAF92_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBI9789480.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(67484..68470)
                     /locus_tag="LAF92_000420"
     CDS             complement(67484..68470)
                     /locus_tag="LAF92_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBI9789481.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            68857..69708
                     /locus_tag="LAF92_000421"
     CDS             68857..69708
                     /locus_tag="LAF92_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBI9789482.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            69701..70072
                     /locus_tag="LAF92_000422"
     CDS             69701..70072
                     /locus_tag="LAF92_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBI9789483.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            70069..70821
                     /gene="fliP"
                     /locus_tag="LAF92_000423"
     CDS             70069..70821
                     /gene="fliP"
                     /locus_tag="LAF92_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBI9789484.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            70824..71096
                     /gene="fliQ"
                     /locus_tag="LAF92_000424"
     CDS             70824..71096
                     /gene="fliQ"
                     /locus_tag="LAF92_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliQ"
                     /protein_id="HBI9789485.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            71098..71880
                     /gene="fliR"
                     /locus_tag="LAF92_000425"
     CDS             71098..71880
                     /gene="fliR"
                     /locus_tag="LAF92_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HBI9789486.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            71870..73009
                     /gene="flhB"
                     /locus_tag="LAF92_000426"
     CDS             71870..73009
                     /gene="flhB"
                     /locus_tag="LAF92_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBI9789487.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIRRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFAAFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            72993..75086
                     /gene="flhA"
                     /locus_tag="LAF92_000427"
     CDS             72993..75086
                     /gene="flhA"
                     /locus_tag="LAF92_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBI9789488.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RNQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            75191..75469
                     /locus_tag="LAF92_000428"
     CDS             75191..75469
                     /locus_tag="LAF92_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBI9789489.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            75462..75818
                     /locus_tag="LAF92_000429"
     CDS             75462..75818
                     /locus_tag="LAF92_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBI9789490.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(75875..76648)
                     /locus_tag="LAF92_000430"
     CDS             complement(75875..76648)
                     /locus_tag="LAF92_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBI9789491.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            76834..77094
                     /gene="dinJ"
                     /locus_tag="LAF92_000431"
     CDS             76834..77094
                     /gene="dinJ"
                     /locus_tag="LAF92_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HBI9789492.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            77097..77375
                     /gene="yafQ"
                     /locus_tag="LAF92_000432"
     CDS             77097..77375
                     /gene="yafQ"
                     /locus_tag="LAF92_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HBI9789493.1"
                     /translation="MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
BASE COUNT        17939 a        20854 c        19902 g        18729 t
ORIGIN      
        1 caggcggagt ttatcaataa tcatagcctg cgtgttcatc ggcctgaggg aaatctggaa
       61 attcatggcg agaaaatttt tattaatacc ggtgcacaaa ccgtggttcc gccaattcct
      121 ggaattacca ccacgcctgg tgtatatgac agcaccggat tacttaatct aaaagaattg
      181 cctgggcatt taggtatttt gggcggcgga tatattggcg ttgagttcgc ctctatgttc
      241 gctaattttg gcagcaaggt aaccatttta gaagcagctt cgctgttttt gcctcgggaa
      301 gatcgggata ttgctgataa tatcgcgacg attttacgcg atcagggcgt cgatattatc
      361 ctcaatgccc atgtggagcg aatcagtcac catgaaaatc aagtgcaagt gcatagcgag
      421 cacgcccaac tggcggtgga tgcactgtta atagcttccg gtcgtcaacc ggctaccgct
      481 tcgttacatc cagaaaatgc cggtatcgca gtaaacgagc gcggggcaat tgtcgttgac
      541 aagcaattac ataccaccgc agacaatatt tgggcgatgg gagatgttac cggcgggctg
      601 caatttactt atatttcact ggatgattac cgcattgtgc gtgatgagtt gctgggagaa
      661 ggcagacgta gtactgatga tcggaaaaat gtgccttatt ccgtattcat gacaccgcca
      721 ctgtccaggg ttggtatgac agaagaacag gccagagaga gtggtgctga tattcaggtg
      781 gtgacattgc ctgtagctgc aattccgcgt gccagagtga tgaatgatac tcgtggggta
      841 ttaaaagcga ttgttgataa taaaacccaa cgtatattag gggcatcact gctgtgtgtt
      901 gactcccacg agatgatcaa tatagtgaaa atggtgatgg atgccgggct gccttatagc
      961 atattacgcg atcagatatt tactcatccg tcgatgagcg aatcactcaa tgatctattt
     1021 tcattagtca aataaactca aaatcagacg ccagaacaaa tattctggcg tctcagagaa
     1081 aagaatctta ttaattccct gttactctat aacttcttaa tcacttcatt gatggtattt
     1141 tatatgttta aaaaatctgt tttatttgca acactattat ctggcgttat ggcattttcc
     1201 gccaatgcag atgataaaat aattctgaaa catatcagcg tctcgtcagt atcagcatca
     1261 ccgacagttc tggaggatac cattgctgat atagccagaa aatataatgc ttcatcctgg
     1321 aaagtcacat cgatgcgaat tgataataat tcaaccgcaa cagcagtatt gtataaataa
     1381 ggatgttcac aatggaaaaa tacctgcacc tgttaagtcg gggagataaa attggcctga
     1441 cattgattcg tctgagtatt gcaattgttt ttatgtggat tgggttatta aagtttgtcc
     1501 cttacgaggc agacagcatt acaccattcg tcgcaaacag tccactaatg tcgttctttt
     1561 atgaacaccc ggaagactat aaacagtatc tgactcacga aggcgaatac aaaccagaag
     1621 caagggcatg gcaaacggcc aataatacct atggtttttc caacggtctt ggcgtcgtgg
     1681 aggtgattat tgctctgctg gttttggcta atcctgtcaa tcgctggtta ggtttattgg
     1741 gaggactgat ggcatttacc acgccgctgg taacactctc atttttaatc accaccccgg
     1801 aggcatgggt acccgcattg ggtgacgctc atcatggttt cccttattta tccggtgctg
     1861 gtcgcctggt attgaaagat actctgatgc tggcaggtgc tgtcatgata atggcagatt
     1921 cggcgcggga aattcttaaa caacgcagta atgaatccag ttcaacgtta aaaactgaat
     1981 attgataatc cgaacactgt ctgttgttca ctttttcagg cgtcaggccc cctttctgat
     2041 gatgaagcct gacgccatat taccggagat atgacagagt gaagatcaat atcactcccc
     2101 tgttaaaaga ttcttgtcgt ctcaggtaaa atatatgatt aatatcaatc atgtattttt
     2161 acagcaagac aaccacgaac aaattcagga ttaaaatgtt ttaccgactc cccaacataa
     2221 cccaaatcaa gcgaactaat ttgtgccatt tctttctctg tcagtgagaa atcccagata
     2281 gcaaaatttt cttcgatacg ctgttgatgt gtcgatttcg gaataacgac cactccccgt
     2341 tgaatattcc aacgcagaat gacttgagaa atcgattttt gatgggcatc tgcaatactc
     2401 tgaagcagat tattttcaaa gggtttatgt cgtccaccac ctaacggagc ccatgcttca
     2461 ggctgcacgt tataatactt catggttgcc agcgcctctg gttgggcaaa atagggatgc
     2521 aactcgacct ggttgaccat cggtttaact cttaccgttt cacaaaagtt cgccagaaca
     2581 tgaggataga aattggaaac cccaattgct tttaatttgc cctcttcata ggcatcttcg
     2641 agtgcacgcc acgcactgaa ataatcgcgc atagcctggt gcaataaata gaggtcgaag
     2701 tactctagtc ccgatttttt taatgatgct tcaatacctg ctgctgccgt atcctgattc
     2761 aacatatcct gcacccacaa tttagacgtt ataaataact catctcgggt acataaacct
     2821 tcagatatag cttcacgaac ggcttcaccg acggcatctt cattaccgta taccgctgca
     2881 gtgtcgatga gtcgataacc tgtgcggata gcgttcaaca ctgactgttt gcacacgttt
     2941 ttatcggtaa cctgaaaaac accaaatccc atcatcggca ttttcagatt gttacttaat
     3001 acggaaaatt ccactgtcag tccctccatt aggcatgaac aatgagtcta cgttaaaacg
     3061 taacctcaaa ttagtacgtg gattttgata tcacttatgc aaaaaattca ttaataatgt
     3121 aggactgaaa cctctctatt tttggggaca acgaagcaga cgctaccagt gcttttgcct
     3181 tcgcccttgc tatttttgat acacttaggg cccagggtat aacgaaaatg tgcgatatga
     3241 caaagaatta acggagaatg agatgatcag gcagaagatt ctacagcagc tcctggagtg
     3301 gattgagtgc aatcttgagc accctatttc aatcgaagat atcgcacaga aatctggcta
     3361 cagcagacgc aacatccagc ttctgttccg aaatttcatg catgtgcctt tgggagaata
     3421 cattcgcaaa cgaaggcttt gtcgtgccgc cattcttgtc cggctcaccg cgaaatctat
     3481 gcttgatatt gcactctctt tgcattttga ttcacagcag tcattcagcc gcgaatttaa
     3541 aaagttattc ggctgctctc cccgtgaata ccgccaccgt gattattggg atctcgcaaa
     3601 tatcttccct tcttttttaa tacgtcaaca gcaaaagacg gagtgtagat taatcaactt
     3661 tcctgagaca cctatttttg gcaactcatt taaatatgac attgaagtgt cgaataaatc
     3721 accggatgaa gaagtcaaac tacgacgtca tcatttagcc agatgtatga agaattttaa
     3781 gacggatatc tatttcgttt ccacgtttga accgtcaaca aaatcggtcg atttgctcac
     3841 ggttgaaact tttgctggta cggtatgcaa accaaccgat tcagagatgc cgaaagagtg
     3901 gacaattaac cgaggactgt atgcctcttt ccgctatgaa ggtgagtggg agcactaccc
     3961 tgaatgggcg cgaaacctct atctgatgga attacccgcc agggggttag ccagagtgaa
     4021 cggcagtgat attgagcggt tttattacaa tgaagatttc gtagaaaagg atagcaatga
     4081 tgttgtttgc gaaattttta tccccgttcg tccggtttag ttttcgctat atctaaatga
     4141 atttataacg agactactgt caggaaattc aataaataat ggcgttgaag atgtcaacgc
     4201 cattattgtt tattagaatg ttactttcat attcttaaaa ttatttcatc gcctcctcgt
     4261 cctttgccgt atcggcatcc accgcggtaa tggtcaccgt cactggcttc atttcgtacc
     4321 cgccataggt cagggcgtta aatgtgaacg tatagacacc aggcttgctg gtactaaatt
     4381 tacgcgtgat ttttccgctc acgaaatggt catttttcga cggcaggaac tgataatcct
     4441 tctccgtcac cccttccggc gtctgaatgt cgacccacat attcccgcca acggggttgt
     4501 ttgccgacat cagtgtcacc tccagcttcg cagtcgattt cccatccgcc accagagatt
     4561 gcatatcaga tgatgcggtg agtaccgcgt ttttatcatc cgcaacgaat ttagattcgg
     4621 cagttttcgt tgatacctga ccgttggtct gactcacttg cgccttgagt gtgtatatcg
     4681 ccgcaacagt agtggtagcg gtgaaaatag cctgcccttg ctcattcgtt gtcgcagtgc
     4741 cattcggttc caggtttaac gaagccgggc tggcagtcag cgtcacttcg ctatctttca
     4801 ccaaattgtt attggcatca gtcaccgtaa ctttatacgt ttgtttggct ttgccgtccg
     4861 ccagcgcata gttgcccatc acctcaatac cggagacggt tgcggtagcc gtatcggcta
     4921 caaacgttat agtctgcgta ttcgaaggct ggctattccc cgccgatgcc gtgatctcat
     4981 acgttccggc agtcactgaa accacctgca actctgcttt cccctcatcg ttggctttca
     5041 cccagacatt atcgcctgta agcggcttga caccccgggg cagattaaag gtcaccaggc
     5101 tccctggaac agggttacca tttttatcct caacggtcgc cgtcagcgta aaggcatctt
     5161 tgccgtttgc tactttttga acggcggtgt ctacctgcag gtttgcctga ccggtgctgg
     5221 catccgcgtt gaatttcacc gtgaccgtct tctgagagtt tttcaccgcg gccgcgattt
     5281 caagttcccc tgcaacggtt gatatcaact caatatccgc tttacctgcc gcattggttg
     5341 ttactgtatt ccccgtcttg ctggtcacac cctgcggtaa attcagcgta acttcttgcc
     5401 cctgcaacgg gttaccatag ctgtccacga cggtcagggt tatctggtta gcagactgtc
     5461 cattagccag ttggttatta accttcactg tcatatcacc aatctcagcc ttagatgcgt
     5521 cacctgcaac attcagcacc agtggctgag caacggcatt ttggccgttc actcgcggca
     5581 tcacagacaa ctgacccgcg gcagatccca gcgttaaggt cgacacgtag accccattac
     5641 ctttctctgt ccagtttcct gctgaaggac gctcactccc cgtgttggcg gcaccgctaa
     5701 acactggtgc atctggcttc atcccggtga ccgggttccc gtacgcatcc ttcacggtga
     5761 aggtgagttc cgtcgtcgtt ttgacggtcg gagccttatt gtccgcaaca agcgtagagt
     5821 tcgctgatga catctctccg gcgatgaccg tcaactgcgc ggcttcggtg gctgctggct
     5881 ggccgttgaa tagcggcatg acacgaagct cgcccgtctt tccgcctgta gttaacgtag
     5941 caacatagga accgtcacct ttttcggtcc aactggaaac ggtcgcccct tcagaggcgg
     6001 tccccgtcaa acttgccgac aacgaaagac cactgatagc gttgccatgc gcatctttcg
     6061 ccaccagcgt cacggttgta ctttcgccgg cttttacgtg atcttctgcg acagagactt
     6121 tcgactggtt tgaagataac gcatcagcca ccacggttac ttttgccgca tttgctgccg
     6181 catcctgtcc atttagcttc ggcataactt ctaattcacc cgccgtagtg ccgagagaaa
     6241 tctgcgcagt ccaggtcccg tcgccattat ccgtccagcc tgatgccgta gaacctacag
     6301 cagctgcgcc cgataatgac ggtgcatccg gattgagacc agttacaggg ttgtcattag
     6361 catcttttgc cgtccagatt gccgtaactg taccgccaac caccggttta tcaggattca
     6421 gtgtgatgga cgaatgtgct gcatcaagcg gcccggcaac aaacttcagc gtttgttgta
     6481 atgtgcccag ctgatactgc tcgctgaaag cacgcacaac aacgtttccg gcacgggtcg
     6541 aagaaaccgt ggcgctgtaa acgcctggtt ttatttccga aatggcacca acggttacac
     6601 cattagtgtc ttgcggaaca aatcgcaagc ggctggcttc tcccgtcacc ggattaccct
     6661 cggagtccac cgcagtcaac gtcaacgtat aggcttgctg accatcagca accacgtcac
     6721 ctgacggctc gttagcgctc agggtggagt ttgccacatc catcatcgtg gcccgcagtt
     6781 ctgcagtgac ggttttgctc atgccatcaa cactaacagt aatcgttgcc tcacctgact
     6841 gcgttccagt agtaaagaca gactgataca cccctgcttc agtttcggtg aactcaccca
     6901 gtgttggctt tgcctgtgat ttagtggcct tcagggtacg agtcacaata tttccagccg
     6961 gtttgaaagt tagttcagtc ttgatctgat ctttcatgcc cgtgactggc tggccctcgg
     7021 cgtcgcgcag agacagcacc agcggtcttt gctcattacc gttagcaagc atttgaatac
     7081 ggctctgacc gtcaagcgtt aacgccgtgc gatcggcgct cataccagct ccggtaatga
     7141 ccacctctgt ctgcacgcgt tttgaggtat tgcctttgtt atcgtaggca accgcagaaa
     7201 tcgcataata attgtctttg cctggacgat aagccgggag cgttacttgc cactgactac
     7261 cctgaccggt aattttgcca ccttcagcca gtaatgacgg cgcttcccac tgcacatttt
     7321 tcagtccgtg agttgctttg ctgaccacaa gccccaggga aagtgtctga ccgcccttac
     7381 cttcaatacg ctcaggcaga gcaatacgga tcacttcaga tttgcggtac tcaagaacga
     7441 tgttgttatt acgctcaacc aggtcatagc ggctgcctgc cagtacccga cgctcgcgaa
     7501 tgctatccgt atcgagttgt ttctccagag gttcgccaat tcggtagtta acttccaggc
     7561 caaagcgagt gtcattctca ccgctcttgc cctgcttatg cccggcgctc agtgtcagaa
     7621 gaggcactgg cgtataggtc acctcggcag aaatagcatg cgggtctttc tggcgcttat
     7681 ctttaccaaa cagcccgact tcatcgccat aatactgttc atacatcagg cttgcgccaa
     7741 gctgcggcca ggcaggtaaa tagccctctg cgcggatatc ccaaccattc gccgggcgtt
     7801 cctgataatc ctcaatatcc ggcgattttt tccagccaga agcccgaata taaccattgg
     7861 cgctcagttt cagataatcg cgccagtatt ccgcaccaac accaatgcgg gtatgactac
     7921 gggataaatc atggtcgata aaggtgttca cccccgccat ccagtcattt cctgaaaaat
     7981 gacgccagcc aaaaccaata tttgactgag tacgatcgtc ggtacgatgt attgctccct
     8041 gagtgaacaa catatttgtt ggcgtatcat aaatcggata aagcatttcc agcgaagaat
     8101 ccttcagcga gaaatcttta tcgacattca gtttgacgcg cgcagtacca tatttcccga
     8161 gccactcctg tatttcctgg ttagctttag ctgtggccat tccggtaata aagttacgtg
     8221 tcgcatcgct atctggctga ctgcttaaaa atgtcccggc atttgcggca aacgacgcga
     8281 catttttctc cacgttatta tcagcagtta ccgtggtatt ttccatgctc aaccgtggct
     8341 gaaccgcatg ctgcgcacgt gccgccatta ctggggtaaa ggtgacagcg agtggaaaaa
     8401 gaacctgaac agagatattt gcccacgcca cgcagcgggc cagaactgaa taacgaaatc
     8461 gtggttgttt atgacctgtt ttataacgcg acattgagat aatttcctga tgaacgaatt
     8521 aacgaacata ggcagacaca aataatggcg tctgcttgtc agaaaaaccg gggatttccc
     8581 gatttccttt tccgcagaaa aggaaatacc tttatgtaag aaagtgcacc gccacctaat
     8641 atatccagtg tctgtagcca ctcatcaggt ggttgggcgc gggattatat ttttatttat
     8701 gggtgaaaat ttagcttttt gtgcaagtca ttaaatagac acctaagtta tattttttac
     8761 tctgcggaca gtgaataatt cattattcag agtgaataat aatggcctgc ctttaattaa
     8821 caactattgg aaatacgtcg aagtaggtat atacgccaga ataaatcgat atatttttta
     8881 tggatattca ttttccttca tcggactgta aattgcggag atttcgaact tgcactaccc
     8941 atcgcaacac tcaaacagat ttatgaattt cgttcataac ccctgacaaa tcctgccgtc
     9001 taatcgagct tttcccttta cgttatgctt ctcatgaacg agaacacaac ttattcatga
     9061 attaaccatt ccggataaac tatggaatca aattcaggca tcgaaaggaa ctggctggtt
     9121 tccgttaata gattaaatat ctattactct cccgagagta aaactatcaa atcaatgagt
     9181 aaccgataca gcctcacaaa ttctttcaga taattaaccc gtcactgcgg cacgttctct
     9241 cataaggcgt gcctgcagtg taatccagta tgtatccggc atagttgacg gtttaataga
     9301 actcaattcc tttcccaggt taccaacaac gatatcttgt ataacacccc aggagatacg
     9361 acattatgat gaaatgaggt atgctcctca gcgccaatca acgcccggac tgggccagtc
     9421 ttgagatcag actcattccc ggaactgact tcaggcgaca tatatcaatg acgttggtgc
     9481 aataaaagat agccgatgta atacatctat tcaaatttgt gatacatgtc aaaaaattgg
     9541 ttgaccaaac tcgttattta tataagggca cttacgaagt gcgctctttt ttaaagcgag
     9601 gaagtaccaa tgaaagagaa taaagtacaa caaatcagtc ataaactgat taatatcgtt
     9661 gtttttgtcg caattgtaga atacgcctat ttatttctcc atttctatta ataacggcaa
     9721 taaactgttc acttcagtga tatttaaaat atgcatcctc tccctttttg taagtaatta
     9781 ttatatccgt gggagaggaa tacacgctgt caggtaatca atcatactgc gataaatcat
     9841 cggccagtaa agtggagata acctccattg tcgaaaaatc catactctca gcaaaaccat
     9901 catcaatcac tcatccaggc gtttatggga gcgtcaccaa tggctgctaa caatgccaga
     9961 gttccccgtt gcgaaaattc cacatccaca aagagtcaca gggattgggt gttgaaatga
    10021 tccggatgag catgtatctt tatggttatg ttataacata acaggtaaaa atgatgaagc
    10081 ccaatatcca tcctgagtat cgtactgtgg tgttccacga caccagtgtt gatgagtact
    10141 ttaaaatcgg ctcgactatc aaaacagacc gtgagattga gctggatggc gtaacgtatc
    10201 catacgtgac aattgatgtc tcttctaaat cgcacccgtt ctatacaggg aagctgagaa
    10261 cagtggcatc agaaggaaat gttgcacgat tcacccaacg ttttggtcgt tttgttagca
    10321 cgaaaaaggg gtcgtgatga aagttcttaa ctctctgcgt accgcaaaag aacgccatcc
    10381 agactgtcag attgtgaagc gaaaaggacg gctatatgtg atttgtaaat ctaatccacg
    10441 ttttaaggcc gttcagggtc gtaagaaaaa acgttgattc aaaattcgac ggattaacga
    10501 tatttgtctg attaataatc agatcggatt aatgttggtg tgtttataac accaacatta
    10561 attttcctgg ggatatattc ctcctgttca tttgaggcca actgcctgac gtttctctcc
    10621 gaatattcca ttatcttaat gttgacttgt tgaccagctt cgcccctgta tgctggcatc
    10681 aactctcttt tagactgaac acgccactca gtctcctccc tttgcggcgc agcctgcatt
    10741 ttcactcaaa ctgttaagat gataaatgtg gtaaatctgt tggtactaac ataaaaacgt
    10801 ttacgccaca ggaacagtct gatccaccgg taaccccgtc gccgacgttc gagtgccagt
    10861 tagagtaacg cacacagata actgcactca gtgccctgac aaagaggcca tcgttcctgt
    10921 gacagctggc agccttcgtt taacttcact taatctggct cttgggggct taccgaacag
    10981 atgacgtaca tacgcccgtt caattttcca tcacttattg taatgaacac ctataaccat
    11041 tttgtgcggc atgttaatcc attaaaattt cttactgatt ggcaaatcat cttcaatgac
    11101 agctcattat agttttatat tctatccctt acccttaaaa cttgtttttt tactagtcca
    11161 tcacacagcg cattaagact attcctaaca cttcagggca aagttcctga ccaatataaa
    11221 atgcaagtaa gaattgaacg ttatattgcc aataacctta tgaaaccaaa tgtctttttc
    11281 ttcttatcaa aaaagcaata ttttcagttt ttctaaatat tgacttaacc attgaattcc
    11341 ttttccgttc acatattgac actcatcggg aaaaaaacat aaatttaagc ccaatcgaaa
    11401 ataattaaac ttaatctcgt ttaaccttta ttgatatgta ctacgtatct tatttacttc
    11461 cggtttacta aggaaactga atgcacctgt aaaaattaca ggtttggaaa gtagtgacat
    11521 ggcaaagtga ttacagcagg gactatgagg ttaaaaacca tatggaatgt caaaaccgtt
    11581 ctgataaata catctggtct ccccatgacg cctacttcta taaaggacta tctgaactga
    11641 ttgtggatat cgacagatta atttatctat cgttggagaa aattagaaaa gatttcgtgt
    11701 ttatcaatct caatacggat tctttatctg aatttataaa ccgtgataat gaatggttat
    11761 ccgcggtaaa ggggaaacag gtcgtattga ttgcggccag aaagtcagaa gccttagcaa
    11821 attattggta ttacaacagc aatattaggg gcgtggtata cgcaggactg agtcgtgata
    11881 ttagaaaaga actggcctat gtgattaatg gcaggttcct gagaaaagat attaagaaag
    11941 ataaaatcac ggaccgggaa atggaaatta tccgcatgac ggcccaggga atgcaaccta
    12001 aatcgattgc cagaattgaa aattgtagtg tgaagacagt gtatacccat cggcgtaatg
    12061 ctgaggccaa gctgtactca aaaatatata agttggttca gtaaactcca ggcaagttag
    12121 ttttaaaaaa tgactcactg ggacatcacg tcctcaattc aactcgggaa gaaatgcaat
    12181 gaaaaaaaag gttctggcaa tagctctggt aacggtgttt accggcatgg gtgtggcgca
    12241 ggctgctgac gtaacagctc aggctgtagc gacctggtcg gcaacagcca aaaaagacac
    12301 caccagtaag ctggttgtga cgccactcgg tagcctggcg ttccagtatg ccgaaggcat
    12361 taaaggtttt aactcacaga aaggtctatt tgacgtggct atcgagggtg actcaacggc
    12421 taccgccttt aaactgacct cacgtcttat caccaacaca ttaacccagt tggatacctc
    12481 aggttccaca ctgaatgtgg gcgtggatta taacggcgcg gcagtcgaaa aaactggcga
    12541 taccgtgatg atcgataccg ccaacggcgt actgggcggc aaccttagcc cgctggctaa
    12601 cggttacaat gccagcaatc gtaccaccgc acaggatggt ttcaccttct ccatcatcag
    12661 cggtaccacc aatggtacca ccgcagtaac agattacagc actctaccgg aaggcatctg
    12721 gagcggcgac gttagcgtac agttcgacgc gacctggacc agttaatctc tctgatgtac
    12781 cagcaggggt agcccccctg ctttattcct ggacggaatt gttatgaaaa agcaccttct
    12841 gcctctcgct ctgctgtttt ccggaatatc tccggcccag gcgctggatg tcggcgatat
    12901 atcatcgttt atgaacagtg acagcagcac gctgagcaaa acgatcaaaa acagtaccga
    12961 cagtggtcgc cttatcaata tccgtctcga acggctctct tcaccgcttg acgacgggca
    13021 ggttatctca atggacaagc cggatgagtt gctactcact cccgccagct tgctgctacc
    13081 cgcccaagcc agcgaagtga tccgcttctt ctataaggga cccgcagatg aaaaagagcg
    13141 ctactaccgc attgtctggt ttgatcaggc cctcagtgat gcgcagcgcg ataatgccaa
    13201 ccgcagcgct gtggccactg cttccgcccg catcggcacc attctggtcg tcgccccccg
    13261 ccaggcgaac taccactttc agtacgccaa cggctacctg acaaatacag gaaatgcgac
    13321 gctgcggatc ctcgcctacg gcccttgcct gaaagccgcc aacggtaagg agtgtaaaga
    13381 gaattactac ctgatgccgg gcaagtcgcg tcgttttacc cgcgtggaca cggcggataa
    13441 caaaggacgg gttgcacttt ggcagggtga taagttcatt cccgtgaaat agatagctgt
    13501 gcagatggat aacgacaatg cctttacgac ggttctcccc aggactgaaa gcccagtttg
    13561 ccttcggcat ggtctttttg ttcgttcagc ccgatgccag cgctgctgac ataagtgcgc
    13621 agcaaatagg tggggtgatt attccgcagg ccttcagtca ggcgcttcag gacggcatga
    13681 gcgtcccgct ctatattcat ctcgccggta gccagggtcg ccaggacgat cagcgaatcg
    13741 gcagcgcttt tatctggctg gatgatggac agctacgcat ccggaaaata cagctggaag
    13801 agagtgaaga taacgccagt gtcagcgaac aaactcgaca gcagctgatg gctctggcca
    13861 acgccccgtt caatgaggcc cttaccatcc ccctgactga caacgcgcag ctggatctca
    13921 gcttgcgcca actgctgctg cagctggtgg tcaagcgcga agcgctgggc accgtactac
    13981 gctcacgtag cgaagacatc gggcagtcca gtgttaacac cctcagcagt aatctgagct
    14041 ataacttggg cgtctataac aaccagttgc gtaacggcgg gagcaacaca tccagctatc
    14101 tgtcgctgaa taacgttact gcgctgcgcg aacaccatgt ggtgctcgac ggctcgctgt
    14161 acgggatcgg tagcggtcaa caggacagtg aattatataa agcgatgtat gaacgcgatt
    14221 ttgccggtca ccgatttgcc ggtggaatgc tcgacacctg gaacttgcag tccttagggc
    14281 cgatgaccgc catttcagca gggaagattt acggcctttc ctggggaaac caggccagct
    14341 ccaccatctt cgacagcagc cagtcagcca cgccagtgat cgccttttta ccggcggcgg
    14401 gcgaagtaca tctcacccgt gatgggcggc tactaagcgt tcagaacttc actatgggca
    14461 atcatgaagt ggatacccgg ggtctaccgt acgggattta cgatgtggaa gttgaggtga
    14521 tcgttaacgg tcgcgtgatc agcaaacgcc cccagcgggt caataagctg tttagccggg
    14581 ggcgcggcgt cggtgcacca ctggcgtggc aggtatgggg cggtagcttt catatggatc
    14641 gctggtcgga aaacgggaaa aagacgcgac cagctaaaga gagttggcta gcaggtgcct
    14701 cgacctccgg ctcattgagt acgcttagct gggcggcaac gggatatgga tacgataatc
    14761 aggcggtggg tgaaacccgt ctgacgctgc cgcttggggg ggcgatcaac gttaacctgc
    14821 aaaatatgct ggccagtgac agctcatgga gcagcatcgg cagcatcagc gccactctac
    14881 cgggaggctt tagttcgctg tgggttaacc aggaaaaaac ccgcattggc aatcaattgc
    14941 gacgtagcga tgccgacaac cgtgcaatcg gcggcacact caacctgaac tcactgtggt
    15001 cgaagctggg cacattcagc atcagctaca atgatgaccg ccgttacaac agccattatt
    15061 acacggcaga ttactatcaa aatgtctaca gcggtacctt tggttcgctt ggcctgcggg
    15121 ccggtattca gcgctataac aacggcgaca gcaacgccaa tacagggaaa tatatcgctc
    15181 tcgatctctc gctaccactg ggcaactggt ttagcgcagg gatgacccat caaaacggct
    15241 acaccatggc aaacctgtca gcacgcaaac agtttgatga aggaaccatt cgcactgttg
    15301 gtgccaatct gtcacgtgcc atctccggcg ataccggtga tgacaaaacc ctcagcggtg
    15361 gggcgtatgc acagttcgac gctcgttacg ccagcggaac gctgaacgtc aatagcgcgg
    15421 cggacggcta catcaatacc aacttgaccg ccaatggcag cgtcggctgg cagggtaaaa
    15481 acatcgctgc cagcgggcgg actgatggca acgctggggt gatattcaac accgggctgg
    15541 aggacgacgg tcagatcagc gccaaaatca acgggcggat tttcccgctt aacggcaagc
    15601 gtaactatct cccgctctct ccctatggaa gatatgaggt ggagttacag aacagcaaaa
    15661 actcactcga cagctacgat atcgtcagcg gccgcaaaag ccatctgact ctctatccag
    15721 gcaatgtcgc tgtcattgag ccagaggtga agcagatggt taccgtctcc ggtcgtatcc
    15781 gtgcggaaga cggcacactg ctggctaacg cacggattaa caaccatatc ggccgaaccc
    15841 gaaccgatga aaacggcgag tttgtcatgg acgtggataa gaaatatccc actatcgatt
    15901 ttcgctacag tggcaataaa acctgcgaag tggcactgga actcaaccag gcgcgcggtg
    15961 ccgtctgggt cggtgatgtg gtctgcagcg gcctctcatc gtgggcggcg gtgacgcaga
    16021 caggagaaga gaatgagagt taacctacta ataacgatga taattttcgc gctaatctgg
    16081 ccagtaactg agctcagagc ggcagtgagc aaaacaacct gggcggatgc accggcacgc
    16141 gagtttgtgt ttgtcgaaaa caactcagac gacaactttt tcgtcactcc tggcggggcg
    16201 ctggatccgc gcctgaccgg tgccaaccgc tggaccggtt taaaatacac tggttcagga
    16261 accatctatc agcaaagcct cggctacatt gataacggtt acaacaccgg cctttatacc
    16321 aactggaagt ttgatatgtg gctggaaaat tcaccagttt catctccttt aactggcttg
    16381 cgctgcatca actggtacgc tgggtgtaat atgaccacca gtcttatcct gccgcaaacc
    16441 accgacgcca gtggatttta tggcgcgacg gtgaccagcg gcggcgcgaa gtggatgcac
    16501 ggcatgttgt cagacgcgtt ttaccagtat ctgcaacaaa tgcccgtcgg cagcagcttt
    16561 acaatgacca tcaatgcctg ccagacctct gtgaactatg acgccagcag cggcgcacgc
    16621 tgtaaggatc aggcctccgg caactggtat gttcgcaacg tcacccatac gaaagcagca
    16681 aatctgcggt taataaatac ccactcgctg gcggaggtat ttatcaacag cgacggagta
    16741 ccgactctgg gcgaagggaa cgccgactgc cggacacaga ccatcggcag cctttcagga
    16801 ttaagttgta agatggtcaa ctataccctg caaacaaacg gactcagcaa cacctcaatc
    16861 catatattcc cggcgatcgc caactcgtcg ttagcctcgg ccgtcggggc gtacgatatg
    16921 cagttcagtc tgaatggcag ttcatggaaa ccggtgagca ataccgccta ttactacacc
    16981 ttcaacgaga tgaagagcgc agactcgatc tatgttttct tctcgagcaa cttctttaag
    17041 cagatggtga acctcgggat cagcgatatc aacaccaaag atctattcaa ctttcgcttt
    17101 cagaacacca catcaccgga gtctggctgg tatgaatttt ctacctccaa cacgctgatt
    17161 atcaaacccc gtgatttcag catcagtatt atctccgatg aatatactca gacaccgtcg
    17221 cgggagggat atgttggcag cggcgagtcg gcactcgatt tcggctatat cgtaaccacc
    17281 agcggtaaaa cagctgccga cgaagtgctg atcaaggtga ccggacccgc gcaggtgatt
    17341 ggcgggcgct cctattgtgt cttcagctcc gatgacggta aggcgaaagt accgttcccg
    17401 gcgacacttt cctttattac ccgcaacgga gctacaaaaa cctacgatgc cgggtgcgat
    17461 gatagctggc gggatatgac cgatgcgctg tggttgacca caccgtggac tgatatctct
    17521 ggcgaagtgg ggcagatgga taagaccaca gtcaaatttt cgattccaat ggataacgcc
    17581 atttcgctgc gtacggtaga tgataacggc tggtttggcg aagtcagcgc ttcaggagag
    17641 attcatgttc aggcgacgtg gcgtaacatt aactaaggcc ctgctgacag cggtctgtat
    17701 gctggcggca cctttgacac aggcgatttc ggtcggcaat ctgacatttt cgctgccgtc
    17761 cgagactgac tttgtcagca aacgtgtagt gaataacaac aaaagcgcac ggatataccg
    17821 tattgccatc agtgctattg atagcccggg cagcagtgaa ttgcgcaccc gaccggtgga
    17881 tggtgaactg cttttcgccc cccgccaact ggcgttgcag gctggtgaga gcgagtattt
    17941 taaattttac tatcatggtc cacgggataa ccgcgagcgc tactaccggg tctcatttcg
    18001 cgaagtcccc actcgtaacc agacaagacg tagcccaacc ggcggcgagg tcagcacgga
    18061 gccggtggtg gtgatggata ccattctggt agtacgacca cgtcaggttc agtttaaatg
    18121 gtccttcgac aaggtgacag gaacggtaag taacactggc aacacatggt ttaagctact
    18181 gattaagcca ggatgtgatt cgaccgaaga ggaaggcgat gcctggtatc tacgtccggg
    18241 agacgtggtt catcagcctg agttacgtca gccggggaat cattatctgg tctataacga
    18301 caaattcatt aagattagcg attcctgtcc ggctaagccc ccttcggcgg actaagactt
    18361 atccatcggt cgagggaaga attccacctc agagccccaa aaatcgttta aatgatgtgg
    18421 aagcgatcgt caatatcacc tgtaacgatg ggctttctca tatgcacacc atagtgttca
    18481 ggagacacgg aaaggtatca ggcagcctgc taacgagtgg tttaatcacg aattagtacg
    18541 taaaatcggt aacggctgga aatcattcaa tactcacact atcgaaagtt ccccagccaa
    18601 ccgcggtacg ttcttacata cgatgtaccg ctgttctctt tacgattttt agctgtactg
    18661 gtgaattatg agcaatctga atccatgcat gacgtgtggt gcctgttgtg catttttccg
    18721 cgtctctttt tactgggccg aagccgacga tgctggcggt actattcccg ccaggctcac
    18781 tgaacaaata tccccttttc accgatgcat gagcggtacc aatcagaaaa acccccgatg
    18841 tattgccctt gcaggaaccc cgggcaaaaa tgcctgctgc acgatatata aaaatcgatc
    18901 gtccacatgc agagaatttg ccatgtctgg tgagaacgga gtcgtcaatg aggcttgcaa
    18961 tcgtgcaagg gctaaatacg ggctgacacc gttataaaca tacaataatt aattgcactg
    19021 ccccgccagc gataatagcg gggcttcgtt tttcaggggt aacaaaaccc gatacttctt
    19081 ctatttgcca gcaacaatgc ctctcttctg tagcgttctt cggatctaca tcatcctgaa
    19141 gtagcgcgat atactcactg actatctgaa tcagaatata aaaagcaatg tttttaacct
    19201 ataaaaatgg cgctgtattt gcgccatttt tatcattcaa tgcattatct gtttgagcct
    19261 aaagggatct cagggtctgg ctcatgagta attctgtttc gtaaatctct gcgaataatt
    19321 tcaatagacc agaaccagac taaatgtcca aaaatttcag aaacattctc ataccacggg
    19381 agatcaaaca gaggtggcgt cagtcccatg agagggaatg aaatcatatg aacaaaaagt
    19441 tgggctaaag cacctgccag taagccctgc cagagtttaa tttttggaaa tacttcagcg
    19501 accacacaat aaccgacagc aaacactatc gaaaagataa tgtgcgtaac accaacccag
    19561 ttaaagacat gcccggcaaa ggtataaaca gccgcattgg gatctgtcag ccccaaccag
    19621 tctcgaagaa aaatatacgg tggattgaga aaattacgcg agcaatcaat ttggcctgca
    19681 gccctgatta atgattccgg gccacacgct gcattaaaca tatccaccgg gctacgtggc
    19741 ggcaatggaa cttcagcccc ccacttcacg aatgcggaaa caacgccagc aatcagccca
    19801 atgaatgcag caagaccata acgtctgcgg ttcggtggag tttgttcaaa tatattcata
    19861 tctaccctgc ttgtaccatt atgttataca cctcttcagg agtattcata aaacaaggca
    19921 aatgtaaaga actgtattgt tttgtataac aagatggttt cctaatcgcc aatgaatata
    19981 agctccatca tttctcccta tttttatatt agaagtgaca gagatttgca gggtgatgca
    20041 gggctgaaaa tcacaggttt ccttattggt ttttgcatcg tacaactaaa gcaataaacc
    20101 agctcccatc atcatcaaca tcccggcgaa catgaattta ttagccgggc tcatagctgc
    20161 attcgcgttt taagaataat cctcctgctg tcgccgacta tgcttaacat ttaaaaaagc
    20221 atcagcactc tcgcaacgca ctcttatttt cccctttaga ataccggagg cctggtatga
    20281 gcaaccaagg cgaatacccc gaagataatc gggttgggaa gcacgagccg cacgatttaa
    20341 gtttgacccg tcgcgatctg attaaagtga gcgccgcaac agcggcgacc gccgtggttt
    20401 atcctcattc tacgctggcg gcaagcgttc cggcagctac acccgcgcca gagataatgc
    20461 ccctgacact gaaggtgaac ggcaaaaccg agcagcttga ggtggatacc cgaaccacgc
    20521 tactggacgc tttgcgtgaa aatctgcatt tgatcggtac caagaaaggt tgcgatcacg
    20581 gacagtgcgg agcctgtacc gtgctggtca atggtcgcag gcttaatgcc tgcctgacgc
    20641 ttgcagtcat gcatcagggg gccgagatca ccaccattga aggcctgggt tcgccagata
    20701 atcttcaccc catgcaggcg gcctttatca agcatgatgg cttccagtgc ggctactgca
    20761 cctccgggca aatttgctca tcagtagcgg tgctaaaaga gattcaggac ggcattccca
    20821 gtcacgtcac ggtcgatttg gtttccgctc cagaaacaac tgccgatgag atccgtgaac
    20881 gtatgagcgg caacatctgt cgctgtggtg catacgctaa catccttgcc gccattgaag
    20941 atgctgcggg ggagataaaa tcatgaaggc gtttacctat gaacgagtga acaccccagc
    21001 cgaggcggca cttagcgctc agcgcgtacc cggcgcaaaa tttatcgcgg gcgggaccaa
    21061 tctgctggac ctgatgaagc tggaaattga aacgcccacc caccttatcg atgtgaacgg
    21121 cctcgggctc gataagattg aagtgaccga cgcgggtggg ctgcgcatcg gcgcactggt
    21181 acggaacacc gacctggcgg ctcacgagcg cgtgcgtcgt gattacgcgg tactctcccg
    21241 cgccctgctc gctggcgcat ctggtcagtt acgtaatcag gcaaccaccg caggtaatct
    21301 gctccagcgc acgcgctgcc cctattttta cgacaccaat cagccctgca ataagcgcct
    21361 gcccgggagc ggctgcgcgg cgcttgaagg ctttagccgt cagcacgcgg tggtaggcgt
    21421 aagtgaagcc tgcatagcca cccatccgag cgatatggcg gtcgcaatgc ggttgctgga
    21481 tgcggtggtg gaaaccatca cgccggaggg aaagactcgc agtatcacac tggctgattt
    21541 ttatcaccca ccggggaaaa cgccgcacat tgaaaccgcc ctgcttcccg gtgagcttat
    21601 cgttgcggtg acgttacctc caccgctcgg cggaaaacat atctaccgta aggtgcgcga
    21661 tcgcgcctcc tacgcctttg ccctggtatc ggtcgcggcg attattcagc ctgacggcag
    21721 cgggcgcgtc gcgctgggcg gagtagcaca taagccctgg cgcattgagg ctgcggatgc
    21781 tcagctatcc cagggggcgc aggccgtata tgacacgctg ttcgccagcg cccatcccac
    21841 cgctgaaaac acctttaaac tcctgttggc gaagcgaacg cttgcctccg tactggctga
    21901 agcgagggca caggcatgaa atttgataaa cccgcagggg aaaacccgat cgatcagctg
    21961 aaggttgtcg gtcgtcccca tgaccgcatc gacggaccgc tgaaaactac cggcacggca
    22021 cgctacgcct acgaatggca tgaagaggcc cccaacgccg cctatggcta tatcgtcggt
    22081 tccgccattg ccaaaggacg cctcaccgcc cttgatacgg acgccgcgca aaaagcgccg
    22141 ggcgtactgg ctgtcattac cgccagtaac gccggggcac tcggcaaagg cgacaaaaac
    22201 accgccaggc tgttaggcgg ccccactatt gagcactatc atcaggccat tgcgctggta
    22261 gtggccgaga ccttcgaaca ggcgcgagcg gcggcctcgc tggtgcaggc acactatcgc
    22321 cgtaataaag gagcttactc cctggcggac gaaaaacagg ccgtcaatca gccgccggaa
    22381 gacacgcccg acaaaaacgt cggtgacttt gacggggctt tcacctccgc tgcggtgaag
    22441 attgatgcta cctacacgac cccggaccag agccatatgg cgatggagcc gcatgcctcg
    22501 atggccgtct gggatggaaa taagcttacc ctctggacct caaatcagat gattgactgg
    22561 tgccgcaccg atctggcaaa aacgctgaaa gttcccgtgg agaatgtgcg tattatctcc
    22621 ccgtatatcg gcggagggtt tggcggcaag ctgttcctga gaagcgatgc gctgctggcg
    22681 gccctcgccg cccgagcggt gaaacgtccg gttaaagtga tgctcccccg cccctctatt
    22741 cccaataaca ccacgcaccg ccccgccacc cttcagcact tgcgtatcgg tgccgaccag
    22801 agcgggaaaa tcaccgctat ctcacatgaa agctggtctg gaaacctgcc cggcggcacg
    22861 ccggaaacgg cggtacagca aagcgaatta ctctacgccg gggcgaatcg tcataccggc
    22921 ctgcggctcg ccacgcttga tttgccggaa gggaacgcca tgcgtgcgcc cggcgaagcc
    22981 cccggtctga tggcgctcga aatcgcgatc gacgaactgg cggaaaaagc gggcatcgat
    23041 cccgtcgagt ttcgcatcct gaatgacact caggttgacc ccgccgaccc gacgcgctgc
    23101 ttctctcgcc gtcagcttat cgagtgcttg cgcaccggag cggataaatt tggctggaag
    23161 cagcgcaacg ccacacccgg acaggtgcgc gacggggagt ggctagtcgg ccacggtgtt
    23221 gcggcgggct ttcgcaataa tctgctggaa aaatcgggtg ctcgggttca cctcgaacaa
    23281 aacggcaccg ttaccgtaga aacggacatg accgacattg gcaccggcag ctacaccatt
    23341 ctggcccaga cggcagcgga aatgcttggc gtaccgctgg agcaggttgc ggttcacctc
    23401 ggcgattcca gtttcccggt ttctgcgggt tctggtggac aatggggcgc gaatacctcc
    23461 acctccggcg tttacgccgc ctgtatgaag cttcgcgaaa tgattgcctc ggcagtcggg
    23521 tttgatcctg agcagtcgca gtttgccgac ggcaagatta ccaacggtac ccgaagcgcc
    23581 acgctacatg aagccaccgc aggcggcaga ctgacagcgg aagagagcat tgaattcgga
    23641 acactgagca aagagtacca gcagtcgacc tttgccgggc attttgtgga ggtcggcgtg
    23701 catagcgcga cgggagaagt tcgggtccgg cgtatgctcg ctgtgtgtgc tgcaggacgc
    23761 atcctgaatc cgaaaactgc gcgcagccag gtcattggcg caatgactat gggcatgggt
    23821 gcggcactga tggaggagct ggcggtggat gaccgtttgg gctacttcgt taatcacgat
    23881 atggcggggt atgaggtgcc ggtccatgcg gatatcccaa aacaggaggt gattttcctg
    23941 gatgataccg accccatatc ctccccgatg aaggccaaag gtgtcggtga gttgggcctg
    24001 tgcggcgtga gcgcggctat cgccaacgcg gtgtataacg ccaccggtat tcgggtacgg
    24061 gattatccca tcactctgga taagctgctc gataagctgc cggatgtggt ttaaggagaa
    24121 acaatgtcat acccgctttt tgataaagac gaacactggc ataagccaga gcaggcgttt
    24181 ctcaccgatg accaccggac cattctgcgc ttcgccgtag aggcgctaat gtccggtaaa
    24241 ggagcggtgc tggtgacgct ggtggagata cacggcggcg cggcgcgccc gctcggggcg
    24301 cagatggtgg ttcgcgaaga tggtcgttac tgcggttttg tctctggcgg ctgcgtggag
    24361 gccgctgccg cttttgaagc gctggagatg atgggctcag gccgcgatcg cgaaattcgc
    24421 tatggcgaag gttcgccgtg gtttgacatc gttctgccct gcggcggtgg gatcacgctg
    24481 acgctccata aactacgctc ggcacagcct ctgctcgccg tgctgaaccg tctggaacag
    24541 agaaaaccgg cggggctgcg ctacgatccg caagcacaat cgttggtgtg cctgcccacg
    24601 caaacccgaa cgggctggaa tctcaatggc tttgaggtgg ggttcaggcc atgcgtcagg
    24661 ctgatgattt acggacgttc tcttgaggcg caggcaaccg cgagtcttgc agcagccaca
    24721 ggctatgaca gccatatctt cgatcttttt ccggcctcag ccagcgctca gatcgatacc
    24781 gacacggcgg tcattttgct gtgccatgat ctcaactggg agctgccagc attgcaggcc
    24841 gcgcgagaag caaaaccctt ttatctcggc gcattgggca gccatcgaac ccacacgtta
    24901 cgtctgcaaa agctccacga gctgggatgg tccagggagg agacagcgca aatccgggcc
    24961 cccgtcggga tattccccaa agcccgggat gcgcatactc tggcactctc cgtgctggca
    25021 gaaatcgcct ctgtacgtct ccatcatgag gaggattcat gcctgccccc gtcgtcctga
    25081 tccttgcggc cgggcgtgga gagcgctttc tcgcctccgg gggaaatacc cataagtgta
    25141 tcggctggcg tcagtccccg gaggttgcgc cttatcgctg gccatttgaa gaaaacggga
    25201 gaactttcga ccttgcgatt gaaccgcaga ttacgactaa tgatctgcgt ctgatgttga
    25261 ggctggctct tgccggcgga ggaataacaa ttgccactca ggaaactttc aggccatata
    25321 ttgaaagcgg taagcttgta tcgctgcttg atgactttct tccacaattt ccgggcttct
    25381 atctgtattt cccacagcgt cgcaatattg caccaaagct ccgcgccctg attgactacg
    25441 tcaaagaatg gcggcagcaa ttggtttaaa tagctgcacc tgcactgcct gatgtcagaa
    25501 cagtattttg atgaattgcc aaggttacaa tggcacaaat gcggcacagg aggaaacgtg
    25561 gtgtatttaa atatggggta actctttgat tttaatggtg ccgataatag gagtcgaacc
    25621 tacgaccttc gcattacgaa tgcgctgctc taccaactga gctatatcgg ccctgaaagg
    25681 acatgttcac gaacgtgaat cacggtggac aaggttaaaa ctaaccgggc gatgcgtcaa
    25741 tggccttgtg aatcaaatgg ctacttttgc atcacccggt tttatttacg cacgaatggt
    25801 gtaatcacca atgccgatcc acttgtaagt ggtcagtgct tccagcccca ttgggccacg
    25861 cgcgtggagt ttttgtgtgc ttaccgccac ttccgcaccc agaccaaact ggccgccgtc
    25921 ggtaaaacgc gtagaggcgt taacgtaaac agcggacgaa tccacttcgt taacaaaacg
    25981 ctgggcgttg cgcatatcgc gggtcaggat cgcatcggag tgttgtgtgc cgtgttcacg
    26041 aatatgggcg atggcatcgt caagatcgct gacgattttg acgttcaaat ctaatgacag
    26101 aaactcatcg tcatactctt cggctttaac agcaaccacc ttcgcagggc ctgcctgcaa
    26161 ctgcgccagt gcagctgcat ctgcgtgtaa tgtcacgccg ctttccgcca tttgtttgct
    26221 taatgcgggc aggaagctat cggcgatgtt tttattcacc agcaacgttt caaccgtatt
    26281 acatgtgctc ggacgctgag ttttcgcgtt gacgatcact tttaatgctt cagcgatctc
    26341 tacactttca tcaacgtaaa tatggcatac gcctatacca cctgtgatca ccgggattgt
    26401 cgactgttca cggcacagtt tatgcaaacc agcgccacca cgcgggatca gcatgtcgat
    26461 gtatttatcc atacgcagca tttcactgac cagcgcacgg tcaggattat caatcgcctg
    26521 cacggcaccc gccggtaagc cgcaggattt cagggcgtcc tgaatcaccg ccaccgttgc
    26581 agcgttagtg cgacacgttt ctttgccacc gcgcaggatc accgcattac cggttttcag
    26641 gcacagcgaa gcgacatcaa ccgtcacgtt cgggcgcgct tcataaatca cgccaataac
    26701 ccccagcggt acgcgacgac gctcaagacg caggccgctg tccagtacgc tgccatcgat
    26761 tacctgcccc accggatcgg cgaggttaca cacctggcgc acatcatcgg caatgccttt
    26821 cagccgtgcg ggcgtcagtg ccagacggtc aagcatcgct tcgccaaggc cattggcacg
    26881 cgcgtcagca acatcctggg cgttagcgtt gaggatgatt tcgctttgtg cttccagttc
    26941 atcggcgatt ttttccagca cgcgattttt ttcgcggctg gagagttgcg ctaatttata
    27001 cgaggcttgc ttcgcggcaa tgcccatttg ttccagcatc agcctgctcc ttaacgggta
    27061 atcatgtcat cacggtgaac ggcaaccggg ccgtattcat atcccagtat tgcatcaatt
    27121 tcttgcgagt ggtgcccggc aatacggcgt aatgcatcgc tgttgtaacg actgacgccg
    27181 tgggcgatat cgcgaccttc gaggttgcaa atgcggatga cttcaccacg cgagaaattg
    27241 ccagtcacgc ttttaatgcc tttcggcaac agggagctgc cgcgttcaag aatggcggca
    27301 gttgcccctt catctaccgt gatttcaccc gccggcggcg caccgaaaat ccagcgttta
    27361 cggttttcaa gcggagtcgc ctgggcatgg aacagcgtac cgacggaaat gccttccatc
    27421 acatcaccaa taacgcccgg cttgctgccc gcggcaataa tggtgtcgat acccgcacgg
    27481 caagccacgt cagcggcctg caatttggta ctcatgccgc cagttccgag gcctgaaacg
    27541 ctgtcaccgg caatcgcgcg cagtgcgtca tcaatgccgt aaacatcttt aatcagttct
    27601 gcctgcggat tgctgcgcgg atcagcggta tacaaacctt tttgatcggt cagcagcaac
    27661 agtttatcgg cacccgccag aatcgccgcc agcgcagaaa ggttatcgtt atcgccgacc
    27721 ttaatctctg ccgtagcgac agcatcgttc tcattgatta ccggaacgat attgttatcg
    27781 agcaacgcac gcagggtgtc gcgggcgttc aggaagcgtt cacggtcttc catatcagca
    27841 cgggtcagca gcatctgccc gacgtgaatg ccataaatcg aaaacagctg ttcccacagt
    27901 tgaatcagtc gactctgccc taccgccgcc agcagttgtt tcgaggcgat agtcgctggc
    27961 agttccgggt accccaggtg ctcacgtccg gcggcgatcg cgcccgacgt cacaataaca
    28021 atccgatgcc cggcggcatg taactgcgcg cactggcgaa caagttcaac gatatgggca
    28081 cggttcagac ggcgcgatcc gcctgttagc acactggtgc cgagttttac caccagcgtc
    28141 tggctgtcac tcatgattct ctgccattca attttaggaa aaatgatatc aaacgaacgt
    28201 tttagcagga ctgtcgtcgg ttgccaacca tctgcaagca aagcatggcg ttttgttgcg
    28261 cgggatcagc aagcctagcg gcagttgttt acgcttttat tacagattta ataaattacc
    28321 acattttaag aatattatta atctgtaata tatctttaac aatctcaggt taaaaacttt
    28381 cctgttttca acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa
    28441 aatgaaaaag agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca
    28501 ggctgcagaa atatataata aagacggtaa taaactggat gtctatggca aagttaaagc
    28561 catgcattat atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg
    28621 ttttaaaggt gaaacacaaa ttaacgatca actgactggt tatggtcggt gggaagcgga
    28681 gtttgccgga aataaagcgg agagtgatac cgcacagcaa aaaacgcgtc tcgcttttgc
    28741 cgggttgaaa tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta
    28801 tgacgtggaa gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac
    28861 cgacaacttt atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt
    28921 cggcgttatc gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga
    28981 cgttaaaaag caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag
    29041 cgatttcgcc attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca
    29101 aagccgtggc acaggcaagc gtgcagaagc atgggctaca ggtctgaaat acgatgccaa
    29161 taatatttat ctggcaactt tttattctga aacacgcaaa atgacgccaa taactggcgg
    29221 ctttgccaat aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct
    29281 gcgtccatcg ctgggttatg tcttatcgaa agggaaagat attgaaggta tcggtgatga
    29341 agatctggtc aattatatcg atgtcggggc tacatattat ttcaacaaaa atatgtcagc
    29401 gtttgttgat tataaaatca accaactgga tagcgataac aaattgaata ttaataatga
    29461 tgatattgtc gcggttggca tgacctatca gttttaatga ttattgccgg atgtgatgca
    29521 tccggcacat ttcactcacg ccgttaactt caccggctcg tcacgaaaat catccgccgg
    29581 ttccagcttc agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag
    29641 ggtgtgctcc agtcgttcaa ccacttcagt gtcttttacc ggaacgctct tccagtcgcc
    29701 tgctttatcg aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag
    29761 ctccatccac cagccccaga actcacgcac ttccggtgcc ggtttcacgt tgacgcatac
    29821 agccagacaa tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc
    29881 tagtgcggta aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc
    29941 aatctcctgt tgtgatgcaa ctgttttacc aaattggtaa aatttagcaa atttttaaca
    30001 caaacgtttt tcgatccagt cggtgatttc ctgaagacct ttgtcaaaat tccgatacac
    30061 cgggttaaat gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga
    30121 gtcctcttcc gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca
    30181 gcgacgtccc agcaatcctt gcacttttaa tgaatagcga ttcaactcca cgcgcaacgc
    30241 ttcgtcggaa gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg
    30301 cacctgttgc tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc
    30361 aaccgctttc agacgcggcg attcaagata tgccagacgc acggcaacgt tagcgccgaa
    30421 acggaaacca aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt
    30481 taagacgtgc tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc
    30541 caccgatggc atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata
    30601 caggctgtaa tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa
    30661 cgggccatcg cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt
    30721 aaactccatc tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt
    30781 tgataaagcc tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat
    30841 gttgtacaac gtagccgcat gcagccagtg tcgaccactg agttgcggat cttcttccgc
    30901 acaggctttt tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc
    30961 acggtagcca atcacagtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac
    31021 aatacgtgcc tggacgtcta ggatttcacg tgggtcaata ccgcgccaga tccacatcag
    31081 acggttaatc atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg
    31141 cggttgtgcg ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg
    31201 gggtttgaac agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact
    31261 tactggaaac tattgtaacc cgcctgaagt taaaaagaac aacgcccggc agtgccaggc
    31321 gttgaaaaga ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct
    31381 gttcgatcca ggtatcttgc gggatatcaa caacataatc atcaaccagc ggacgaccag
    31441 ccggttttgc aaagatggtg acaaagtgcg cttttggata catttcacga atcgcaaccg
    31501 cagtaccacc ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg
    31561 cgcgtttcag cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg
    31621 tatcgacatg acgaataccc agttcacgcg ccagtaacgc acccggtacc agaccgccac
    31681 ggcttacggc aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac
    31741 gtgcatggat ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc
    31801 cagcctgttt atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat
    31861 agagatctgg cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc
    31921 ctctccctga cgcgggataa agtggtattc tcaaacatat ctcgcaagcc tgtcttgtgt
    31981 tgacaacatt ttctgctaac cctgtgacct gcaatactgt tttgcgggtg atcgacaagg
    32041 agacttaacg tgtctgaact gtctcaatta tctccacagc cgctgtggga tatttttgcc
    32101 aaaatctgtt ctattcctca cccgtcctat catgaagagc aactcgctga atacattgtt
    32161 ggttgggcaa aagagaaagg tttccatgtc gaacgcgatc aggtaggtaa tatcctgatt
    32221 cgtaaacctg ctaccgcagg tatggaaaat cgtaaaccgg tcgtcttaca ggcccacctc
    32281 gatatggtgc cgcagaaaaa taacgacacc gtgcatgact tcacgaaaga tcctatccag
    32341 ccttatattg atggcgaatg ggttaaagcg cgcggcacca cgctgggtgc ggataacggc
    32401 attggtatgg cctctgcact ggcagttctg gctgacgaaa acgtggttca cggcccgctg
    32461 gaagtgctgc tgaccatgac cgaagaagcc ggtatggacg gtgcgttcgg cttacagagc
    32521 aactggttgc aggctgatat tctgattaac accgactccg aagaagaagg tgaaatctac
    32581 atgggttgtg cggggggtat cgacttcacc tccaacctgc atttagatcg tgaagcggtt
    32641 ccagctggtt ttgaaacctt caagttaacc ttaaaaggtc tgaaaggggg tcactccggc
    32701 ggggaaatcc acgttggcct gggtaatgcc aacaaactgc tggtgcgctt cctggcgggt
    32761 catgcagaag aactggatct gcgccttatc gatttcaacg gcggcacact gcgtaacgcc
    32821 atcccgcgtg aagcctttgc gaccattgct gtcgcagctg ataaagtcga cgtcctgaaa
    32881 tctctggtga atacctatca ggagatcctg aaaaacgagc tggcagagaa agagaaaaat
    32941 ctggccttgt tgctggactc tgtagcgaac gataaagctg ccctgattgc gaaatctcgc
    33001 gataccttta ttcgtctgct gaacgccacc ccgaacggtg tgatccgcaa ctcagacgtg
    33061 gcaaaaggtg tggtcgaaac ctccctgaac gtcggtgtgg tgaccatgac tgataataac
    33121 gtagaaatcc actgtctgat ccgttcactg atcgacagcg gtaaagacta cgtggtgagc
    33181 atgctggatt cgctgggtaa actggctggc gcgaaaaccg aagcgaaagg cgcatatcct
    33241 ggctggcagc cggacgctaa ttctccggtg atgcatctgg tacgtgaaac ctatcagcgt
    33301 ctgttcaaca aaacgccgaa catccagatt atccacgcgg gcctggaatg tggtctgttc
    33361 aaaaaaccgt atccggaaat ggacatggtt tctatcgggc caactatcac cggtccacac
    33421 tctccggatg agcaagttca catcgaaagc gtaggtcatt actggacact gctgactgaa
    33481 ctgctgaaag aaattccggc gaagtaatta tttgatttgc tgccggatgg cgtttaatcg
    33541 ccttccggca gtttcatcct tcattatcct tcgataaaag ccatccctgt aaatgtccgt
    33601 ctcgggttgc cacgttcaat ctggtgatgg aacattcgcc gctgcgattt cagcaccgcg
    33661 ctattttcct gttgctgctg ctccagcttc caggcaatca gcaatcgtgc cagccgcttg
    33721 ttagcatgct gactacgctc tgactgaacc ttcacgctaa taccggatgc caaatgcgtg
    33781 gcgcgtaccg ccgagtcggt tttattgaca tgttgaccgc ccggccccga cgaacgcaac
    33841 gtctcataac ggattgcatc cgattgttcc tgctcatcag cggtaaaacg cccaatgccc
    33901 agaaaccagt ttttgcgccc atgatgaggc cgatacggac tcggacaaat ccactgaata
    33961 gtgccgcacc agctttcgct taatgcccat gcgttatcgc catccagaga aaccagagct
    34021 gaacgcagcg tgtcagagta gcggcccgtt tctgtttcca acaccgttac cgcgacgtct
    34081 tgtcgggcag cttctttaat taatcgctcc agcgcctttt tcactgcgag acaacattct
    34141 tccggcccct gcgctgagga gagttgtagc aagatcatcc ttttttccct ccactgtttt
    34201 tgagcgttag caccggacgc agccgcgcga caggaataat taaaccggcc tgcaccagac
    34261 attgcaccac gctttcagcc gatttatagg cttgtggcgc ttcttcaaag atgagttgtt
    34321 tatcgcgaca aattacccga ctgccaagtt cagtccgcga gagttgcgtc accgtgtatt
    34381 tcgctgccag acgcccttta cactcggtgc gcccccattt acgccctgcc ccatgcgcca
    34441 gcgagtggag cgttttttcg ttcgcaacag gctgtacaat ccaggagtaa tcaccacgtg
    34501 aaccgggaat aatcaccaga ccgttgtcat ccggcgtggc acctttacga tgcaaccagc
    34561 cctgttgatc gccgatttga cacgcgctaa caaagttatg cgccacatcc agaaccggac
    34621 ttcccgtggc cttaacttgt tgcattatgc gcaaagcgat catctggcga ttaatacacg
    34681 caaacgccag cgcatcgtca tgttccgcaa tataccgtag cgcgtcgtca ctgccttgag
    34741 gcaaaccatg atgcgagaac gaggcaatat gccgctgtaa aatagactgc cccacccccc
    34801 gcgagccgct atgaaccaac agttgcagat gttgtgcatc cagacctgcc agtgcaaaca
    34861 gttcagcgtt gatgatttga tcaacttgtt gcagttcagc gaaatgattg ccgccaccga
    34921 tagagccaag cgagctgcgc cacggatgct gtgcaaatgc tgacggcagg ttttcctcca
    34981 gccagctttc ttcagcaacg tcatccagat ctgataatcg cttttcaaac ttatcggcgt
    35041 tgtatttgcg agtgagaata tctgtttgcc atagcgccat accgcagccg atatcattgc
    35101 cgaccagtgc cggataaaaa cgaccaacag agaagaacgc tgcgccaatc ggatagccgc
    35161 gtccgggatg taaatctggc atccccacta cgcgctgcat gttgggtaaa tttgccgtgg
    35221 tgtgtaattg ttggatcgct aaactctcga tccacaggtc atcagatgcg atggtaaata
    35281 ccgcatcgga taagggacga atataattgc ccatatacca ttccgtaaat agactcgtca
    35341 tacttcaagt tgcatgtgtt gcgacttgaa ttattgagag taacaattaa aaaatcagga
    35401 aatggcagac gaatccaggg caagaaaaaa agaattaatc ctggaaaggt ctgcaaagag
    35461 tcattgataa aggatttgtc atgatttacc tccttgcagt ttgtggaata agaaaacgcg
    35521 cacatactag gcttgcgtta ttttcattgc aagctggatt taatattgct gtttataacg
    35581 catataaaaa ttaataaacc actctccccg acattcaacg cgttgctgct taactgtctg
    35641 aaaaccataa cgttcaaaaa agggttttgc ggttatgctt gcgtccaccg taagttcgga
    35701 ttcagactta atcaaaggtt ttaacaaagc gctggcaacc ccacggcggg tgtattcagg
    35761 gtcaacaaat aacatatcga tatagtgttc aacgcaggta ataaagccga ccagttttgc
    35821 attaatgact gcaacccgca cttgtgattt cgcgagtttc tccttccagc gagattcgtc
    35881 aatctgcgcc caggcggcaa tttgttgtgg tgaataatgc tgactggcag tcatcgtaac
    35941 cgctctaatg aaaatagcgc atagttgctg aaaatcgcca ggctgatagt ctcttatttg
    36001 tatgttattc ataatcccag caccagttgt ctttccattt gcgggtcaag caacgtcaca
    36061 tgcagcccca ccagtcgcac accgcgcccg ccgcggcgtt catcccaggt tttacgcgcg
    36121 gtggcgatta agtcagcttt attcagccgc ggccagacgt gctcctgagt ggtttgctga
    36181 aaatcgtcga actttaattt caccccctgg cgagcaatca gtaaatcagg tttcaccttt
    36241 gccagacggc gttcaagttc cggatacagc cgctcgataa tcgcttcaca ttcagaccag
    36301 tggtggatat cttccgccat cgtgcgttcc acgccgacgg attttcgcaa ccgttcgctg
    36361 ttaacgtcgc gctcgtcaat cccctgacta cgctcccaca aaatgcggcc aaatttgcca
    36421 aagcgtttaa gcagcatcac cagatcacac ttttgtacat caccgcaggt ccgcagcccc
    36481 atcgcttcca gttttgccgc cgagactttg ccgacgccgg ggatttttgc cagcggtaag
    36541 gtttgtaaaa atgccggaac ttctgccggc gtaatcacaa actgaccgtt gggtttattc
    36601 atgtcggagg cgattttggc gagaaacttt actggtgcca cgcccgcaga tgccgtcagt
    36661 tgcagctcgt tgaagatcgt ctggcggatt tcctgggcga tgagggtcgc agaaccgtgg
    36721 caatggacgc tatcggtgac gtcgagataa gcctcatcca gtgacaacgg ttcaatacgc
    36781 gaggtgtagc gcgagaagat ttcacggata tgatttgagg cttctttgta ggcgtcaaag
    36841 cgccccggaa gcaaggtgag atgcgggcat aatttgagcg ccatccctgt cggcattgcg
    36901 ctacgtacgc caaatttacg cgcgggataa ttggcggtgc tgatcacccc ccgacgttcg
    36961 cggctgccgc caatagcaat agggatatcg cgcagggcgg gattgtcgcg catctccacc
    37021 gctgcgaaaa agcagtccat atccacatga atgattttac gcattgctca cctctcaata
    37081 ctggtaaagt atacagtgat ttcagggttt gagaaatgcg taaagattca gcatctagcg
    37141 cgttcgctgt gaaagcacct gctgcttatc cagcttttgc accagcggct gcaccacttt
    37201 atccccatgc tgaccaaagt attgatacag cgtatcggac gcacttttgg tcagcaccat
    37261 aatctcaatg cgccggttgc ccgcgctttg cggatttttg gaatccagca gcatctggtc
    37321 cgccattgcg cttacctgca tcactttatc ttccggcatt ccggcctctt ccagcacccg
    37381 acgagctgaa agcgcgcgat cgcccgaaag gttccagttg ttgtagatat tgtttttgta
    37441 ggccatcgca tcggtatgcc cggtaataat aattttgtta tcgagcgagt cgaacactgg
    37501 cgcgagctcc accagcagcg ttttaaagaa cggcataatc tgcgcgctgc cgcgttcgaa
    37561 catattgcga ttctggtcgt ctttaatcag cacacgtaat ccctggggaa caatctccat
    37621 ctccagattc gcttccatat gcgcatcacg ggcgatggtg ttgatactgg ttgccagctc
    37681 gccaagctcg gtggcggatt tcttcttcag cagcgccgtt ttctcattca catcacgcgc
    37741 ttttttctcc gtctcttccg gcaccgccac ggtggcaggt tttggcggat gagacgggct
    37801 gattttattt aaaggcgaca gcccgccacc attgaaaatc gactgcccgt gcagcgcggc
    37861 gataatgctt tcacgctcgg atttactgac gctgttgaca atccacagcg tcataaacag
    37921 cgccatcatt gccaaagtaa agtcggcgaa cgccaccttc cacgccccgc cgtgatgccc
    37981 ggcatgattc tttttaatct gccgccggac gatggtggtt tttgccccac gatcgcggcg
    38041 gttgcccgtc tttctcatgc ttattcttgc tccagcatcg cattcaccca cgcgtcgagg
    38101 ttggcgaagg tcggtttgga cgccagatgc agtagcttac gcccggcgtc caccgccagc
    38161 agcgtcggtt tgccgcctgc ctgcgccacc agcacggtgc gcacgcattc cagcaaagaa
    38221 tgttccgccc gcgcctgttg ttccatggcg ttagcgagcg ggtccatcag gcagtagcag
    38281 ataaagacgc ctaaaaacgt ccccaccaac gctgccgcca ctttcaggcc aatcagggcg
    38341 atagagccgt caatcgactg catggtgatg atgatgccca acaccgccgc acaaatgcca
    38401 aaccccggca tcgcttcggc ggtacgttgc agcgagcgcg acggcgtgag cagcgactct
    38461 tccgcagtgt ccagttcctg atcgagaatg ccttccagtt catgggcgtc aatcttgccc
    38521 atcgccatca ggcgaaagtt atcggcaata aaggtcacca gccgcttctg ggttaacacc
    38581 agcgggtatt tctggaaaat ggtgctctct tccggttgct caatatgttg atccagcatg
    38641 cgcaggccac cgttttgcac catttcgagc aactcataca ggcacattaa tagctgacgc
    38701 tgaaactccg gcccgagctg ttttttactg atcacgcctt taatttgatg ggcaatctct
    38761 ttcagtacat gtttcgggtt accaataatc atcgccccga aaccggcacc caaaataatg
    38821 atcatctccg ccggttgcca gatagccact aattgcccac ccgcttcaaa ataaccgcca
    38881 aatacgcagc cgagcaccac cagtaaacct aatatttttt gcatgatttc ttcactttaa
    38941 ttcggataga caaaatcacg aattttctgc gcgatctgtt tattcagttg acaaattcgg
    39001 gcctcggtta gccctaatac cagcgcaatc tctttgagat tcatctcatg ctggtaatac
    39061 atggagagga tcagttgctc tttttcgctt aattgtgaaa gcgcgtgttg caacatttgc
    39121 tgggtgacaa actgatcttc cagttcccgg cctgcaagcg gaataccagg cccttcgccg
    39181 ttcagcagtt cgtccaggct ggcgagcgtt tccgcccctt ccagttgctg atattcgaga
    39241 taatcttcat gcgagatccc ttccacggcc agttctgacc actccggttc gtgccccagt
    39301 tttttgcgcg tttcgcgaat cagatctttc atctgatgat atttctgtcg cagctggcgc
    39361 ggtcgccagt cgagagcgcg cagctcatcc aggatcgcgc cgcgaatacg gtgcacggca
    39421 tagcccgcaa aaccttcgtc cggcagcccg tagcggcgga tggcattaag cagccccatt
    39481 aacgccgttt gttccatatc ctgacgatca ataatgcaat tacactgcgg ggcaagttgc
    39541 cgcaccactt tacgcaccaa cggtaaatag gcctgcaagt agtggctctc ctgctgcggg
    39601 gttaataccg gcgcggcagc aaattcttcc gactcgaata catcctgtag catatttata
    39661 tccgcctggt gattactgga agaccacttt tttcaccagc aaatccttat acggtgcgct
    39721 cattttgcga cgctgaagat ctttctttaa cgcgtcagaa atgagtttgc ggatggcgga
    39781 tattttcatg ccacgcaaat cttcaaattt catttccgtc aggcattcaa ccgcaatact
    39841 ttgatacagc ggctcctgat gttttatttt ttccgcttct ttgtcactgg cgaccaccat
    39901 cactaattcc gccgacaaat aatggtccgc gccgttattg tcatgcagcg tgacaatggt
    39961 ttccggcaac gtcacaaaca cgcttttgct ttcatccagg gtttccaccg tttccgtctg
    40021 cgcggcggca gtcggttttc cttttccggc ataatgatgc cagacacccc atccagctcc
    40081 ggcccccacc accaacgcca gaacggcgct gaccacgccc gccaccacga ttttcttcat
    40141 attgccttta cactttgatc aaaacggatt cgttctgttg aaattgaggc gcatcatcaa
    40201 gctgtaaggc ggcactcacc tcttcctgct gatggcccga ctgttgttgt tgctgcgact
    40261 gcccttcgga ggaaacctgt acgttcacct ccataaaatt ttgcgccgtc agatgctggc
    40321 ggagatcgtc gctaaactgc tgtaacgcgc ggcaaacttc actttggttc gcgccgatgt
    40381 gcaccatcag tttgccgttg tcgagttgta cggcgatctc gagcttgcca agcgacggtg
    40441 gatcgagacg gatggtggag atctgctgtt gtttgttgag ctgaaagtgg atctgatctt
    40501 tcaacagcgt cgtcagtttt tcgcccagtt catccatcga aagcgtggct ggtttggcga
    40561 caatctcctg atgatcgctg accagctccg gcgtcttaac ctgcaacacc gcacgcgcaa
    40621 taactggttc gctgtccggc actttttccg ttttacgctc gacctgcggg cgcgcggtca
    40681 cacgggttag ttcaggtttc gggcgagctg gctgtgttga gacgcgcggc tgcgtcggcg
    40741 taatggcatg taaatcttca ctagcgtaag tcgccagtct ggcctgctgt tcgggcttcg
    40801 cctgcggcaa ctgactgatc agttcctgca actgcggcgg caacggcgtg agttcctgct
    40861 gttgcgtctg gcgttgcggc gcgttttgca ccaccgcttt cgtcaattgc tggataaccg
    40921 gcgatgttgc gacgtttcgc ggctgcggcg tgtcctgatg cggcgcggca ggttgcggaa
    40981 gtaacagcgc cattagcgcc tgcatcgcca ccggatcgtt ctcctgcggg cgcgtcgctt
    41041 gttgctgact ggttttagcg ctatgaacgc gctccgccac cgccgcgacc gccattttcg
    41101 gcagggtaaa cgcgggcgcg ttttcaccgc tcaccggcgg taaaatatcc gccttcaacg
    41161 aagcggtttc cgcaagcgtt ccgagcgtgg caagcaatgc cggattcatg acttctccga
    41221 aagtaaatta acaattgagt aggcgagcac accttcctga tgccgctgat gctgttccat
    41281 acgcgccttc aacacttctg cgcgctgctc gcgccgttta atcagggcat taaacgcgcc
    41341 gtacagtcgc gccttcaccg cacacaactg cgccgcgtcg gtttccgccg ccgcgtaatc
    41401 gtgaaactgg cgtatcaact gctgatgcag tgctggaagg cgtcgccagc gttgcttgtc
    41461 gagagcttca ttcatcgcct cgaccagtgc gaaaagttgc ctgcgttgtt tttccatcgt
    41521 cttaacccag cttgcccgaa agcccttccc agccttcgcg caggttgctt aagatcagca
    41581 tcacttcatc gattttctct gccgacagtt cgccgctggc ctcatacaat cggtaaacgc
    41641 agtggtcata gagtcgcgaa aggttgacca ccagctcgcc gccagtttca aactccagcg
    41701 aactggtgag cgcattgaga atgtcgatgc atttattgat gctctgggct ttcttctcga
    41761 aacgccgccc ttcaatatgg cttttggcgc gttccagttc gtccatcagg ccagaaaaca
    41821 gcaccagcac cagttccaga ggcgatgccg ccgcagtacg cgccgcgaga tcgatctctt
    41881 tatattgcga atagccgtcc tgtacttggt acatctttag ggatattcct tctgagttac
    41941 gcgaatgccg ccatgctggc tttcatggtg tacacctcga ccagcgtgtt ggtgtactcc
    42001 tcgagataac ggtcgtagtt ggcgttatag gtatcagtaa gctgatcgcc ttcgtcctga
    42061 attttgctca tctgatcgtc aatgttctgc tggcgcaggg tgatgatgcc gttgctggag
    42121 tccgtgtagg tgttaataag gtcgtccatc tgcgccacca tgctgttgtc gccaacgaaa
    42181 atagaggtca gaccgtcagg atttttcgcc atttcgtcgt tgaactggtc ggagtcaatc
    42241 tgtaagtggc cgtgagaatc gagggtgatg ccatagtcaa caatcgacac gccgttgtag
    42301 ctggcatggg cgatgtcatc gagctggttc gccagtgaac tgagtccggc gtcgcctgca
    42361 aataccccgg cgctggtact gtcatcaccg tgggtggtta gcgaatcaac cgtatcaatc
    42421 aaggtgttat aggcatcgac aaaggtctgt actttctcct ggctggcgct ggaatcttcg
    42481 ctgatgttaa aggtagtgag atcgctgtca gaatcgctga cttcggtgaa agtcatggtg
    42541 acaccgggga tcacatcatc aaacgtattg ctgctgttgg tgatctcagg ccccgtggcg
    42601 ctgccaagat gaataatcgc gtcctgagcg gaggaaacat ccgtggcaac gggcgcgctg
    42661 gtgctatcgt tgctggcatc atgccccgtt acgctcaccg aaaacgcgct ctgcgccccg
    42721 gtgctatccg aggtgagcat aatcgttgtg gtgccgtcgg tttttaccag cgccgccgac
    42781 acgcccggat tatcatcgga gtcgttaatc gcattcacca gttcactggc gtcgataaaa
    42841 ccgtcgccgt caccattctg atctgccgcc gacagatcga tatccatggt gctgtcgccc
    42901 atcgtcagtt caaaagtgcc ggtggcggaa aaggcgtcgt cgcccatact gaacgtggtt
    42961 tgctgcccct gtgccagttg ctcaacgaaa aatgagtagc ttcccgcctg cgcctgggaa
    43021 ttggcggaaa cggtcgccga gtcattattg ctggtggcgg caaacgtcac cgggccgtcg
    43081 gtatcgctgt tcagcgcgtc aacggcgctc tgaaaatcgc tcaacgctga actgagcgaa
    43141 tccaggccgc tgctttcagc atccagctct gtttgcttct cctgcaagtt tgccgcctga
    43201 gtggcgacat ccgcatacgc gatttcttgc gcaatggttc ttgggttgat cattgatgac
    43261 tccggataac gatgtcaggt tattggcaaa aagcgtgcca ataattttta tgtgaataat
    43321 caataaattg aaaaagaggc ggaaatggcg tttccgacct ggcggaaaag tatctgccgc
    43381 agaaagcaca acaccgcccg caggcggtgt tgttcgtggc cggataaggc gtttacgccg
    43441 catccggcaa gtccgccatt actgcagcag gctggaaacc atgctggaca tgctgttgga
    43501 ctgtttcagc atggtgatgc cagtctgcat cagcacctgc tgtttggtca tattggacgc
    43561 ttccgtcgcg tagtcggtat ccataatgtt accgatagcg acttcggtgt tgtcctgcat
    43621 actggtcagg ttgttggcgg tgtcgttcag acggttgata gacgcaccca gcttggactg
    43681 gatctgcgaa acgtcatcca tcgcggtaga gatgctggtg atcatctggt tagccgagcc
    43741 agacgcggtc agctcagtac cgccagaaac acctgcagta cccgtggtgt ctgcctgatc
    43801 cgcgctaaag gagttgctga tagcagaaag atcggtcacc agcgtgttca gttggctgga
    43861 gatgttcacg gtcatggtgt cggaagattc cgcgcccacc tggaaagtca ccgcgctctg
    43921 gaacagaccg tcagtaccgg tgttgctggt gccggaaaca ccgaacaggt tggtgccgcc
    43981 gtaggtggtg ttttgcagca tgtcagacat ctgctgaccc agttcgtcgt attcgtcctg
    44041 catcgcctgc agatcgtcgt cgctgtaggt gccgttcgct gcctgagttg acaggtcttt
    44101 catacgaccc agcacatcag acatttcatc gaacatacta tcggcggttt gcagcatcgc
    44161 ggttgcgtca ttgatgttgc tcagcgccac gcccataccg ctggactgtg ctgtcagacg
    44221 gttagcaatt tgtttgcctg cggcgtcatc ggcagaagag ttaatccggt tacctgtcgc
    44281 caggcgttcc atagatgtag acaaagaaga gctgcttttg ctgattgcat taacggcggc
    44341 cattgaggcg ttattggtgt taatagataa catgatactg ctccttggga ttaatcgttt
    44401 tcgtttcgat accctgtaaa aacgacacct aaggagcaaa cttaaaaccg ccgttaaaaa
    44461 aatttcactg tgatttatct gatgccactt ttagctaaaa aaagcagagt ttaaattcag
    44521 ataaaaagat tattagcaat atctgccaca tggccaggta tcaaaatatg tctttatcaa
    44581 acgcttgata tagttaagat taccatcaat taacgcgttt tcttctcaat gtactgatgc
    44641 caaaatttac attgcgttgc aaatagtaca catttggcta ttgtacctgt cctgtcattt
    44701 cgttacattt ccacgccata tagtgattaa gaataatctt attaccataa aatattagtt
    44761 atcacgcaga aaaatcatct tctcagggac gtataagact gtgctttatg caaagcacta
    44821 tcaggaaaca tttatccctt gctaaggtaa actcaactat gagtattatt atcaacaact
    44881 ggcggatgga cccgtcgctt aatgccttaa tacattgcga aacgggtgaa acacgtcgtc
    44941 tcggcgaata ccattttatt ttgctggaaa cattagcaaa aaatgccgat gtggttttat
    45001 cgcgatctta tttatgtgcc gaagtatgga agaaccgtat tgtcggcgga aacagtctgc
    45061 cgacggcgat ccatgcgttg cgcgttgcta tcgatgatga tggcaaacaa cagaacatta
    45121 ttaaaacaat tccgaagaaa ggttacttat gcaataaaga gtatgtgtct ttgccggact
    45181 catcgcctgc gaaaaaacta attattaccg accagataca ggaaacagta ccagaagaga
    45241 tatcttctac aacaccgcct gtgcctgtta gaaaaaaaca taaagtaata atggggttag
    45301 cgttagccgc ggctgttata tttattggct cgaccgtggg gtattcacac ttgaaaagta
    45361 cacctgatgc cccgcaactg gtaaaagagt caattaacag tccaagaata aaaatatttc
    45421 accttagttc tggaaaagaa aataatcccg taccgttact gtcacaaacc ctcgcaccag
    45481 ggaaagacaa acttgataat ttattgtcag cgcataatat gacaatgaca acctattata
    45541 aatatgttcg taaccgactg gaaagtgata ttgttctgcg caaccagtgc aatggtagct
    45601 ggcaattaac ttttaatgtg gaaggctggc agaacagcga tattaatagc gcgatgtatc
    45661 agaatttaga gaagttgtta aatactttgc agaaatgctg atgagtgcca atatatgccg
    45721 gatgcgacgc taaacgcgtc ttatccggct gataattacc ccaataaatt cctcaccgtc
    45781 tggctggaaa ttcgcgcctg accattcacc gcctgcaata aaacctgata gttatgattc
    45841 gcgctatcca gcacgccgcc gaggttttcc gacgcctgca ccgcgttttc cgtttgcggg
    45901 aagcgcgaca tgccgtcgat caactgccgc gccttttcct gctgtaccgc catctgcgca
    45961 cgctgctgat tcatgctttc taacgtctga gagatccccg caccgccctg ttgcaggctt
    46021 tgagctaaac ggtctgcctc agacggctcg gcaaaagttt tcagcggcat aaacgccgac
    46081 ttatcaccat cggtgcgcac gcttaaggtg cgctcgatat gaggccactg tttttcagtg
    46141 gtggagaaac tgatgccatc cgcttgctga tgtacctgca cgcccacgcg gcgcagggcg
    46201 ttggtcagcc gcgtctgata ctgcgcggcg ctgtcatcct cagacagcat caccgccgaa
    46261 agctgagtct gccgcccgtc agagacgctg aacatgcgcg ttcccggtgt agggttgtgt
    46321 accagattcg ccagatccgg cgagtgaaag gtcacccgcg cttcgccctg caacaccggc
    46381 tgcaactggc ggtccaccgc accgccggaa agcgcggtgc gtttgtccag catctgcatc
    46441 agcgccgtac tctgcgcctg cccaccctta cgctggctat gacgataatc gagtaactgc
    46501 tgctccagtt gcccgaggta gtgatccgcc tgctgcaacg tggtgagctg gtcgttaagc
    46561 tgcacgctgt aacgctgcgg gcgagtggta atcagcggcg aggccggata ttcactcgcc
    46621 gtcgccggga gtttttgccg caccgcctga gtgggcgcaa cggttgacga cgataccgca
    46681 gcgctatgcg cgccgccggt ggcgagtgac gaagatgtaa gtccgacctg catactgttc
    46741 ctgaaagagt tagagaacgc tgaacaaatt caactcactc actttcgagt aagtcttgag
    46801 ggtagcttcc attgccactt ccagtccggt aaaggtgatc gacgccgggc cgtaatcgag
    46861 atcctgtaat gaaccaatca actcatcatt agaagtagag atatcggtct gcgcatcgct
    46921 caacatcgac atggtgttct gcgtctcgcc gagcgacgcg atcgaggcgt tgaggtcgtc
    46981 cgaggcggta tccaccacat caacggcgtt ctgaatatcg ctctgcacct gcggatcggc
    47041 ggggtcagcg ttggggtctt gcaactcctg agagagcgaa ttcagggtgt tgagcacgtc
    47101 taaattgctg ccaaagaaat cactcgccgc cacgttggtg tccacttcca cgccgttcga
    47161 caccgtggtc tggcggtggt cgctgttacc ctgatagctg tagctgtcgg tgacgccatc
    47221 gccgtcatcg tccactgcca caatcggcgg ctgatcgttg atggtgccgc caaacacgta
    47281 gtgcccgtct tcgtcctgat aattgagcgc cgccaccatt gattcggtga gcgactcaat
    47341 atcctgcccg aggcttgcga gtgaatcggc ggtattggtg ccattcgccg cttccagcag
    47401 gtcgtcgcgc accgccagca ggctgttgtt gacgccatca aggatcgact cctgctggct
    47461 taagcccgcc gacgcggagt cgatattgct ctgatactgc tcaatcgccg actgctggcg
    47521 gtttaactgg gtgattcgcg tggcggcgat cgggtcatcc gacggctgca aaatctgctt
    47581 gccggttgcc atctgctcga caacatgcgc cagatcgcca gagagattat taaaactttg
    47641 cgtcatcgag acgtaggttt gttgggtagt tactcgcatt ttcgcgctcc cgcttagctg
    47701 cacatttcca gcaccgaatc gaaaatctcg gccccggcgg aaatgacttt cagattggct
    47761 tcataaattt gttgataggt gatcaggttc accgcctctt cgtccatgct gacgccgctg
    47821 acgctgctct gctggttttg cgctgcggaa tagacattgg acgcggcatc gacttccgtc
    47881 tggttttgct gactgtaaat gccgatattg ctgatgattg acgagcacgc ctgcccgacc
    47941 gtcacgctgc cgaggttggc tatttccagc ggttcggtgg agatattgat cagcgcctgc
    48001 aggttgtcgc tgttgccgct ttcgtccggt gaactggaaa acgccagctc atcggcggtg
    48061 atattcgggt taacggtcag cgggccatcg gcattgctgg cgtcataaat aaacagcggc
    48121 tcgccggggt taccgttgag atcgtagccc tgcgccagct ggttgttgac cgcatcggcg
    48181 aactgcgacg ccatgctgtt gatggtgtcg gtcagcggcg tcagcacatc gttttgataa
    48241 tcaaacagtg cgcctaatga accgccggtg tcggtggtca tcgtcgaggt ggtgccagcg
    48301 aaggtcagcg acatggtcga cgtgccatcg gcgttggttt ccagcgcgat ggtcgaactt
    48361 tgctgcccgc tcaccagcgg ctgaccgttt ttcagggtaa cgttgtagtt gccctggtcg
    48421 tcgatattga cctgcacatc catcatcccg ctcagttctt ccaccatctg gtcgcgggcg
    48481 tcgtacagcg cggaggcgtt atcgccattg gcttcagcct gagcgatttg ctggttatag
    48541 ctggcgatgc cgctggtgag cgtattgatt tgcgacacca tcgcctgctg ctggctgatg
    48601 atttccgtgc tttgcgagtc gatgtaatcc agcgtgttat caatacgcaa cgacagcgcc
    48661 ccggcttcac tgatcacctg ctcgcgcagg gcggaatcat cggggctggt ggttgcttcg
    48721 ttaagggcgg cgaagaagtt atcgaagccg ccgctcaggc tgctgttatc gtcgctcagt
    48781 accgcttcca gttggctgag atacccctgc tgggtgctgt aatagccgta atcgctggcg
    48841 gcataccaca cctgattcac ctgatactgg ttggagacgc ggcgaatgct gtcgacctgt
    48901 acgccgttac cggcgctgtt cggcgaaccg ccgctggcac cgatggtgct gatctccgcc
    48961 acctgacggg tgtagcccgt ggtcatggcg ttggcggtgt tttgcgccgt gacgttaagc
    49021 tccacctgcg cggttgatgc accgctgtag ccgatgttaa tcatgtccat gttggatcct
    49081 taccggtaaa tcgtgccggt taaaagcggc gtgaagatgg ggattcttaa attccgtaaa
    49141 ccgggtgccg gacgccggat gcggcgtaca tacgggcaca aacataaaaa tatgtgcgaa
    49201 ctcgtaggcc tgataagacg cgacagcgtc gcatcaggca ccgtgcaccg aacgccggat
    49261 gcggcgtaaa cgccttatcc ggcctacaaa cgggcgcaaa cataaaaata tgcgcggact
    49321 cgtaggcctg ataagacgcg ccagcgtcgc atcaggcacc gaacgtcgga gcctcaccta
    49381 tgcttcggcg acaactgctt cacaatcatc gccgcgatcc caatcccgtg ctgctgcgcc
    49441 agggtgttac acaactcatc gtcataaaac ccctgcatca tgcgcaccga atcgctgtta
    49501 aatgggttat ctttcgaatc gaacagctcg ttggttttac gcatctcttt cagcatgttg
    49561 cgtaaaaaaa ttgcctcaaa ctgctccgcg gcctgcttga tatcattggc ctgaaccttc
    49621 ggccccatta acgcatcgct gccgtcaatc ccgccgctgg cgttgacttt catcagatca
    49681 cctccaggtc ggcatccagc gcgcccgctt catgcagcgc ctgcaaaatt gacataatgt
    49741 catccggcgt cgcacctaaa ctgttcaacg cattgaccag cgttttcagg ctgctcgact
    49801 ccggcaaact caccacgccc ggacgggcat gattcaccgc gatattgctc tgcggcgtta
    49861 ccgccgtgcg cccgcccgca aaggcattcg gctggctgac gttgctggtt tcgttaatcg
    49921 aaacggtcaa actaccgtgc gacaccgccg ccgcgtgcag cgcaacgcca tcgcccatca
    49981 ccaccgtgcc ggtacgcgag ttaaacacca cgcgggcgcg gattttctcc gcctgcacct
    50041 gtacatcgtc cagttgcgac ataaacgcca cccgcgcgcc ggggctggtg ggcgcgcgca
    50101 cggtaacatt ggtggaactc tgcgcggtgg caatgccgcc aaacgaggcg ttaatcgccg
    50161 ccgcaatgtt gttggcgtct ttaaacgacg ggcgtttcag gttgagggtg atggtgtcgc
    50221 ccatctgaaa atcactgggg atctcgcgct ccaccgttgc gccgttagga attaacccgg
    50281 cggtcggtgt attaaccgtt acgctcgagc cgctggcccc ggtggcgttc atcccaccta
    50341 ccaccacgct tccctgcgcg agggcataca cttcgccatc cgcgccgtgc aactgggtca
    50401 gcagcaaggt gccgccacga atacttttgg catcgccaat cgacgaaacg gtgacatcaa
    50461 tggtctgccc gcgcgagtac atcggcggca atgtggcgct caccgccacc gccgcgacgt
    50521 ttttcacctt cgggtcgatt ttattcggca actgcacgcc aaactggcgc agcatattgg
    50581 tgatggtctg gttggtgaat ttgacctggt ttttatcacc ggtaccgtcg aggccgacca
    50641 ccaggctgta gccgaccagt tggttttcac gcacgccctg tacgttgacc agtgactcca
    50701 gcgattgcgc cagcaccacg ccgggtagcg ccaggctcac ggcgatcgct gcggccttta
    50761 tccagttttg cattgcactc tccggcatta aatcgggaac agaggatggt taaacagccg
    50821 cgtcagccag cctgcggcgt tggcgtcgct taacgcgccg cgtccggcat aggagatacg
    50881 ggcgtcggcg atccgctggg aggaaaccga gttgtcgttc tggatgtcgt cggcgcgcac
    50941 caggccgctt aagcggatgt actcatcgcc ctggttgagg gtgagccatt tctcgccgcg
    51001 aatttccagg atgccgttgg gctgcaccgc atggacggaa acggtgatct ccccgcgcag
    51061 gctgttctgc tgctgggaag tggcgctgcc gtcaaagtcg cggttggcat cgatagagcc
    51121 ggaaagctta tctttggtgt gtccaaaaat ggtcggcgcg ccgatatcga cggtgttgct
    51181 tttgccgaag ttggttttcg cctgtttgct cgactgggtg gattcttcga ggatcaccgt
    51241 cagaatatcg cccacgcgat aagcccgccg atcggcggtc agcgaccagt tgtagccagt
    51301 ttcaaataca ccgccagcac gcccgtctgc cggaggcggt gcttcggttt tcggtggcgc
    51361 aaagtatgcg tcatcttttt tgaccagaat ggcctgcgat tcacacccgc taagcagggg
    51421 aagcagcgcc accagccaga gataattttt cacgttctgc ctgtttagtg ttgcaggcca
    51481 cggggtggcc ggttgtttgg tgggggcgga gctgtttgtt tcccgcctca ccctaaccct
    51541 ctccctcaag ggagagggga ctgatttgtg cgcgttgtta catcgcgcac cgcttttctc
    51601 cctctcccca aaggagcctg gggactgatc tgtgcgcgtt gttacatcgt gcaccgcctt
    51661 tctccctctc cctgtgggag agggccgggg tgagggcatc agaccgcacg ttgcccccac
    51721 gcccttacag cgtctggctg atgtactgca acatatcgtc cgccgccgac accattttgg
    51781 cgttcatctc gtacgcacgt tgcaccgtaa tcatcgccac catttcgttg acgatatcta
    51841 cgttcgagcc ttcgagggca ttgtcctgca aagtgccaag cccatcttcg cccggcacac
    51901 cttcggtcgg ctgaccgctg gcggcggttt ccagatacag gttgtcaccc tcagcggaaa
    51961 gcccagccgg gttagcgaag ttaaccagcg tcagttgccc taactcggtc acgtcgctgt
    52021 cgccaggaag aatcgccgtc accgtgccgt cctcaccaaa cgcaacattc gtcgcccccg
    52081 ccggaacgtc gatttccggc tgcaatggca agccctgtga gttggtcagt acgccatcgg
    52141 cgttcacctg caaattgccg tcgcgggtat aggcgatatc gccgttggcc ttttgcacct
    52201 gcaaaaatcc ctgccccata atggcgacat tcatcgcgtt gtcagtggtt tcgacgttgc
    52261 cttcggtgaa ggttttctgg gtgccgacaa tgcgcacgcc gctaccgaat tgcagcccgg
    52321 tcggcatgat gttgttctgg tcgagcatcg cgcccggcgc ttcctgggtc tggtagaaca
    52381 gatcctgaaa catcacccgg tcacgcttga agccggtggt gttaacgttg gcgatgttgt
    52441 tggcaatcgc gctcatttca gcatcctgcg ccgagaggcc ggttttgctg atccataatg
    52501 ctgcgttcat ggtggtaaat tccgtgttat ttttgggccg cggttgccgg atgcggcgtg
    52561 aacgccttat ccgacctacg atttgagcac aggcttgtag gcctgataag acgcaaagcg
    52621 tcgcatcagg caccgcagca tagctgccgg atgcggcgtg aacgccttat ccggcctacg
    52681 atgtgcggtt acgatccgcg caataagcgg ttgcccgcgt cgctgatatc ttcggcggtt
    52741 ttcatcatct tgatttgcgc ttcaaactgg cggttcatag cgatggacga catcatctcg
    52801 ctcaccgccg agacgttgct gccttcgaga aaaccgccgc tgactttgat gttttcatcg
    52861 cgctgggccg gaacgccgtc agccgttatc agcatccctt cagcattttt cgccaggtta
    52921 gcgaccggga tatccaccag cttcaggcga tcgatatcca tcgtcgcggt cacgtcgccg
    52981 tcatcgggcg tcactgacag cgtgccgtcg ctgccaaacg aagcaatggc attcggcggc
    53041 agaatgatcg gcccgttatc tcccagcacc agattgccgt cgatggtcag ttgcccctgg
    53101 tcgtcctgct gaatgttgcc gttgcgggta tacacttcgt taccgttttt atcctgcacc
    53161 gcaatgtaac ccgcgccctg aatcgcaacg tccagcgggc gttcggtttt ctccgcaaca
    53221 cctgtgctgt cgttcacgcc gctggcccct tcctgcgcca tatagcgcgt gtcgaagccg
    53281 ccacctttca ccttgtcgtt ggttgccatt gccatatcgg cgcgaaaacc attggtgttg
    53341 acgttcgcca ggttgttggc gctgatctgc tgctcgtaca gggtctgaga agcaccgctc
    53401 agggcggtat aaatcaaacg gtccattaca ttgcctggaa cagcgcgtca tcgagctgcg
    53461 tgctggtggc gataactttg gtgttggcct gatagttacg ctgggcggtc atcaggttga
    53521 ccagttcgct ggtgatatcg acgttagagc tttcgagcac gccggacgac agcgtgccgc
    53581 aggtgccgga gccgggaacg ccaatcagcg gggtgccgga ttcgccggtt tgtacccatg
    53641 cggtgccgct caccgcttcg aggccgtttt cattcgggaa agtcgccagc accacttgcc
    53701 cctgcaacat gcgctcgccg ttgctgtagg tggcgtaaac tttgccatcg tcatcgacct
    53761 gcacaccgtt ttgcgtggcg gaggcgtagc cgttggcggc gttggtggtg acagaaaatt
    53821 cagagccgta ttgcgtacag gtcgagtaat cgacggttaa gtcgatgggc gcggcggcgt
    53881 cggtctgaaa ctcaatagtc tgcggcgtag ttggcgaggt cagcttcccg gtgttcgggt
    53941 cgaaggttaa ggtggtcgcc ggaacaccag tctgttgctg accgtcgaag gtgtactgca
    54001 cttcccaggt gttgtcgctg gttttggtga agtactggca taccgagtgt tcgttgccta
    54061 atgagtcata caccgtggtg gtgtagctgt cggtatacga gctgctgttg gtcgcgtcga
    54121 acggtacggt tgtacggtcg atagccgcat cgctggcatc gaagttggcg gtaaaggtca
    54181 ggctgctgct ggcctgcgcc ggaatattgc cggttttgat ctggatatcg gtgaccgtac
    54241 cggtttgcag ggtgccagtg tcatcgaccg gataaccctg caaataagcg cccgaggcgt
    54301 tgacgatata gccgtttttg tcggtttcaa acgatccggc gcgggtataa gaaatgttgc
    54361 ccgcgctgtc gcaggtaaca aaaaagccat catcgttaat cgccagatcc agcgcgttgc
    54421 cggtggagac cagcgaaccg ccgcgcgaaa tactttgcgc cgtacccgcc acgctgacac
    54481 ccatcgcctg gcttccggca tacatggcgg aaaactgggt ggtcatcgac ttatagccca
    54541 ccgttccggc gttggcgatg ttgttactga tcccgtccag ctgttcgtta acggcattca
    54601 gccccgtcgc ggcaatttca taactcattg tttcgtcctg ttcattcaaa ttttgacagc
    54661 gtgcccgttg ttttgccgga tgcggcgtga acgccttatc cggcctacaa aatcaggcca
    54721 catcactgtc ggcatcgggt acgccaaact ggctaatcat ggtgaacggc acttcgccga
    54781 cgcctgcaac gttgagcacc ggagagctgc cgtcgagcgg gatgcgcaca tcttccactt
    54841 cgccggagac ttcaatcggc acctcttctt ccccggtgtt ggtgaccacc gagacgtcgt
    54901 aatcgcccgg cgggataccg taatccgcgg gattcaccga gaaatccacc tggcctggcc
    54961 cgacggacga actcccttgc gggatcagcg agacggtgta gtcatcgcct gcggcatcgg
    55021 taaagtggag atcggcggtg gtacaggcgt cgtccagcgt agcgcgacca ttaatggtct
    55081 ggccgctcac ctgcaaggcg gtggtctgca ccataatgtc gtcgcccacc aggttaccga
    55141 gcgccatcac ctgaatgttg ctcatcagca ccgcgttggt gttggcctgc acgctcatct
    55201 cttccatcat cgccacctgc gccatcgacg acagctggtc gatatattcg gtggcgtcgg
    55261 tcgggtcggt cgggtcctgg ttttggatct cggcgacaaa cagcgtcagg aaggtatcga
    55321 cgggcgagct atcgctgctg ctttgcgtgt cactggccgc cacattactg ctttcctgcg
    55381 tggccgttgg ctgcggcgat tgcgcattca gcgcgagggt attcatcagg cttctcccag
    55441 tttcagcaaa ctttgctgca tacttttcac gttgttgagc acatcgacgt tggtttcgaa
    55501 atcgcgcgag gccgacatca tgtccgccat ctgctccacc acgttgacgt cgggatacca
    55561 gacgtcaccg ttggcgtcgg cgagcggaga atgcggttca tagcgtttca ccgccccgcc
    55621 ggtttgcagc acgtcctgta cctgcacgct ggcaccgtca atggcatgac gatgtgtacc
    55681 cgccagcagg ctgtggtgat acaccgcggc aaacaccggg ctgcgcgctt tatacgcctg
    55741 cgcctcgctg ctggcgggcg cattggcatt cgccaggtta ctggcaacgg tattcagacg
    55801 tagcgtttgc gccgtcatcg ccgagccgga aatctgatag atatcagtaa atgacatgct
    55861 tatttccctt caatggcctc tctgatgccg ctgatttgca gattcagaaa ggtcaaactg
    55921 ctttgataat ccatactatt ttgtgaaaac cgcgcctgct cgctgttcag ttccacggtg
    55981 ttgccatctt ccgacggctg catcggcaca cggtactgga catccaccac cgcgttattt
    56041 gcccgttgca tctccctggc aaaatcaata tctttagcct tgaaatttgg cgtatcgaca
    56101 ttcgccagat tcgcagtcag tagctcggtc ctctcaagac gtaacttcac tgcctgcgga
    56161 tgtactccca atgcctgttg aaaactgatc cccatttcct gcgtcactcc tgcttcaatc
    56221 cgcatacgct aagcaatcgc catgccattt tttaagtcac tgatgagaaa gggtttatga
    56281 gttttcaatt ttgcaggcgg aagttttatt tccttctggc gctagcctta ccgggctacg
    56341 ccgccgtcca tcccgtgcaa cacagcgcgc gggaacaagt caacgcccag gtgctgaatg
    56401 ccgcgagcca aaaaattgaa tcgctggcgc agcagcggca gtggcatgac tatcgctaca
    56461 cctttaagat ctacattccc tcgcagattg cgaccgccgc gccctgtact aaaacacccg
    56521 gcgtcacgct gacttcgcct gcggaaatcg ccctcaaccg gatgaacttc accgtcagtt
    56581 gcccgcaaag ctggcagatg aacgtggcgg tgcgcccgga tgtactggtg ccggtggtaa
    56641 tgacgaagtc actggttgcc cgcgatacgc cgctcaccgc gaatgatgtg gagcttaagc
    56701 cctacaacgt cagcgcccag cgccgcgatg tgttgatggt tctcgatgac gcgattggtt
    56761 tcagcagtaa acacgccctg caacccggca ggccaatcac caaagaagaa ctcgtgtctc
    56821 cggtgctggt cgggcgcgat caaccggtga tgattgttta ccagagcgcg gggatcagcg
    56881 cctcgatgcc cggcgtggcg ctgaaaaatg gtcgtaaggg cgagatggtg aaagtgcgta
    56941 acgccagcag ccagcgggtc atcagcgcaa tggtggcgga aagcggcgtg gtgacaacgg
    57001 taagcgcgga gtaatttgcg ctaacgatta aagttttgcc gcctgttaac cgctttaccg
    57061 cagtaaccca cccaattacc gagacgaggt tgcgatgaaa atcaccccga ctatgccagg
    57121 caatcgcccg acagccacaa cggcaggctc cagccagccg cgcaaaacca cggcgaccac
    57181 caccacgacg ctgagcgccg acgacattac tcaggcggga ttgcagtccg cccagcaaac
    57241 gctgaacgac cagcaagaga gcgatatcga cagcgacaaa gtggcgcaaa tgcaggcgat
    57301 gctggcctcc ggcagcctgc aagtggacac cgaacagtta gccagcgaca tgctgagttt
    57361 tttccagaac taagaggccg atgaagtgag tatccggtta caacaggtga aggcgttgct
    57421 gcagggaatt cgtgacgacg acgcgctgta tgattccctg cgcgaacgtc tccagcgcca
    57481 gcggatctgc atgattcgcc gcgccagcga ggagctgctg gcagtaaacg aggagataac
    57541 gcatcactat gagcagttgc atggacatag ccatcaacgg cacagcctgc tgaaaatgct
    57601 cggtgttagc gtaaatcgtg acggtctggc gcaggtattt gcctggcttc cggcggtaca
    57661 aaaagcggca gcacagcagt tgtggcaacg ccttgagcaa aaagcggaac gctgcaaagc
    57721 ctacaacgat aaaaacggcg agctgttgat tcgccagtat gaatttattc agtcgttttt
    57781 aggcagcgaa gcagattttc tctatcagga ataacgcgaa taccttcccc atagcggaaa
    57841 tggattttcc gcctgatttc caatcccttg tttttgatta caaaaatacc cggcacgctt
    57901 attgcatatc ctgcacagca ttaaagactg gaagccacct atgagtttta ttgaatgtta
    57961 cgaaccggaa tacgtgcgca aatttttagc gcgtcacccc ggttcgcctt tatacgtccg
    58021 caaggtgtgg aaaaacgaga ttcgcctgag cctgtcattg accgatattg ccagctgcga
    58081 agcggtgctc aacgatgtca gggcctttga tttacagctc acctaccgcc ctgtggaaga
    58141 taattccgca gagctttccg cgcgcgatgc gatagtaaac caggtgattt tgagtaccct
    58201 cacgctccgt gatttaaccc cggagctttc cctgtatgct gtcgggattt tgctctcccg
    58261 cgccagcaaa atgccgggca gagacggaga catcctggcc cgtttgacga cgctgccact
    58321 ggcgctggcc gatcatgcga aaaaaggtac gctacaggca cagtacgccc agctcccgcc
    58381 cgtgccacag ctggcgcgcc agttgatgac gctgctcggc ggttgtgcgt ttgactggtc
    58441 gattttgccg gaaagcccgc gcaaagcctc gctgccgttg caggttactt tactgacgct
    58501 gcatgacgcc aacagcgaag ccctgttaca acagcagctt cagacgcagt ggcaaaccac
    58561 ctggcaacag cattttgcca ccgcgccctg gatgatgcgt aactggctga tttaccgcgt
    58621 ttaccacgat gtgattgggc agactgacgg ggcggattat ttcccgctgg tgtgcgattt
    58681 ttatctcctc cgcacgttaa tcagtttatg gacgctggat ggttctccgc tgcggcagga
    58741 ggatattttc gccctctttg ccatgtttga acgctggcga gccagtgaaa atgcgctgtt
    58801 ggttcgccag caaattcagt cgttatgtgc cgccgacccg ctgctttcag cgttttcgct
    58861 gctaacgtga gtcgctctgt gcgcgctttg cccctcaccc taaccctctc cccaaagggg
    58921 cgaggggacc gatcgagcac agtgttaaaa tcggcgctga ttttctccct ctccctgtgg
    58981 gagagggttg gggtgagggc atcagcgcgc acattcatca cattcaccca ttaactcacc
    59041 caaacaccat gccaacacca accatcatca aaacactcaa cacccaccgc gaaaaaccgt
    59101 ttgtcagcgt cgtcacgcca acctggaacc gcggcgcatt cctgccgtac ctgctctata
    59161 tgtaccgcta tcaggattac ccggcagacc gccgcgaact catcatcctc gacgattccc
    59221 cacaaagcca tcaacacatc atcgaccgcc tgaccaatgg cacgccggaa gcctttaaca
    59281 tccgctacat ccatcacccg gaaaaactgc cgctgggcaa aaaacgcaac atgctcaacg
    59341 aactggcgcg cggcgaatac atcctctgca tggatgatga cgactactat ccggcagata
    59401 aaatctctta caccatcgct atgatgcaaa aacaccgggc gctcatctcc ggttccgacc
    59461 agatccccat ctggtacagc catatcaacc gtatctttca aagccgcagc tttggcccac
    59521 ataatatcct caacggcacc ttttgctatc accgtaacta cctgaaaaag caccgttacg
    59581 acgatgattg caatttaggc gaagagaaaa gtttcaccga caatttcagc gttaacccgc
    59641 tgcaactgcc cggcgagcgc acgattttgt gtatctcgca tagccacaac acctttgata
    59701 aagactttat tatcggggcc agcacgccag taaacacggc gcttacggat atcgtccgcg
    59761 atccgctgct gcgcaatgct tatctgagtc tgcataacgc cactcatcac caggcgatca
    59821 accatcaggc gatcgatcag gtggtcgtcg tgaatctgga taaacgcccg gatcgcttac
    59881 aacagatccg tgaagaactg gcattgctgc atattccacc cgagaagatc acccgccttg
    59941 ccgcctgtga agatgaaaac ggtcaacggg gccgccgcct gtcgcacttg caggcgctgc
    60001 gactggcgca acagcatggc tggcaaaatt acctgttgct ggaagatgac gcggttattc
    60061 tcaagcagga aaaacatatt caggtgctga acacgctgct ggcgagcctg gcgaaaattc
    60121 cctggcaggt gatgatcctc ggcggcgaaa tttcgcaggg aacgatgcta aaaagtttgc
    60181 cggggctggt gcacgcgcgg gactgccgca aagtgtgcgc ttatctggtg aacagccgct
    60241 actacccgca actggcgcag cagttgagca acgatgagca ctcgctggaa gacgtctggc
    60301 agccgctgct acgcgccgac aaatggctgg cctgttaccc gagcctttgt taccagcgac
    60361 cgggctttag cgatatcgag aaaaaagtga ccgacaacat tagtcactat ttcaataagt
    60421 tacctgtatc cacaaagcca tccgcattac ccatcgccga caccatcggc ttttttatgg
    60481 aaacctcatt ccactacgcg ctgtatcgtc caatcatcac cgctctacag gcgcaggggc
    60541 agagctgcac actagtgatc aatgaccgcg tgtttaaacc gtttcttgat gagatgctgg
    60601 aaacactgaa aaacatcgac gatccgcagc ttaaaggtat gcgcttgtct gagatgcagg
    60661 cacatagtca gcgggtgaaa tgcctggtca gcccctacca cacgcctgcg ctcaatggcc
    60721 tttcacccgt gaacatccgc gccatgtacg gcttagctaa agagacgtgg aaccatgcag
    60781 actggaaccg tttttatcag cgtattttgt gttacagcca ctacagccag cgggcgctgg
    60841 aacatttcgg caatgcaaaa gtggttggca acccgcgctt cgacgcctgg cataacggca
    60901 cgttcgaccg cgctctgccg gaaaatattc aacccgattc ctgtaaacct acggtgctct
    60961 acgcccccac gtttggcgca ttaagctccc tgccccactg ggcagaaaaa ttgggccgct
    61021 taagcggtga tgtgaacctg atttgcaaac tgcaccacgg caccagctcg cgcccgcaag
    61081 aagctgcatc gctggccctg gcgcgcaggc acttaaaaca gctcaccgac tccgcccgcc
    61141 acacgctggc gctactggca aaggccgatt acgtgctcac cgataacagt gggtttattt
    61201 tcgatgcgat ccacgttgat aaacgggtga tcctgctcga cttcccgggc atgacggaac
    61261 tgctcgacgg tgagaaaagt tattccacgc cggagagcgc cgaccagcgg attcgcgaaa
    61321 tattgccggt ggctcatgag gttgcggaac tgcgttactt attgtcagag gcgtttgact
    61381 gggggtcggt gcaggcgcgg cttacggaga ttcgccatca ctattgcgat gcgtttatgg
    61441 atggcaaggc gggagaacgg gcggcgattg tgattgtgga ggcgctgggg taaaccgcgc
    61501 aactgcctga tgcgcttcgc ttatcaggcc tacggatcga tcacgtcctg taggccggat
    61561 aaggcgttta cgccgcatcc ggcaagaaaa tacatctctg ttttagtact ttataaaagc
    61621 cctctaatca cctcaataat ccccgttgtc gacaccgacg gcgtgcgggg gaaatagacc
    61681 acctcacaaa tatacgcaaa gctgtcaaac ttccctgccc agtcatcccc catcactaaa
    61741 atatccgcac tgtacccgcg caaatactcc gctttctgct ctagcgattc ttccagaaat
    61801 acgccatcca cgcacgccag cccggcgatg atcgccatgc ggtcatcctg atggtaaacc
    61861 ggtgccctgc cctttttggc gatgttcagg gcgtcggacg atacgccgac cagcagccgc
    61921 tcgcccaatg cgcgggcgcg ttgtaaaagg cgcagatgac cgacatgaaa cacatcgaag
    61981 gtgccgaagg tgatgaccgt tttcatggct actccgctaa tttgtgccgc agccagcact
    62041 gcacggcgat gtcgtccacc gctttttgcg ctttcgccgt ggcgctttct tgttgctcgc
    62101 ggcgtttgtc gtccagcgtc agggcgacta ctttctcttc gcacgccgcc gccgtgaggt
    62161 ttttctgcat tcgcgtggct ttgatattgg ccagcgtttt ttcctgctcc tgccaggcga
    62221 ttaccttgcg cagggtgcct ttgtagccgc tgacgttttt cagcgactcc accgatgccg
    62281 ccgcgcccgc agacgttttc tccaccaggt agcctaacgc cttgatattg ttgtcatagc
    62341 ccgcgcaaat ctgtttttgc tttgccagct cgatggtttt gtctttgacc gatttatccc
    62401 gcaggcgctg caactgcgcc agggtgtcga tgatttgtcg catagttact gttctgcctt
    62461 tttgacacca ggaaagagaa tttgcaggcg gctttgcacc agttcgaggg aggcgggttc
    62521 gcgcatctcc tggcgcaaaa atcgctcaat ggccggaaac attttcaccg ctttgtcgac
    62581 gctggcgtcg gcacctgcca catagccgcc gagcgggatc agaggtttga tctccatata
    62641 ggcggcatag ttttgcttta acgcgcgggc ggattgctga tgttccaggc tggtgacctg
    62701 gttcatgcag cggctgatcg actggccgat atcaatggcc ggatagtgcc ccgcttccgc
    62761 cagtttgcgg gttaatacga tatgcccgtc cagcaccgcg cgcgcgcagt cgacaatcgg
    62821 atcttgctgg tcgtcacctt ccgccagcac ggtgtagatg gcggtcatcg atcccgcgct
    62881 ttcgctgttg cctgcgcttt cgaccagttt ggggatcatg ccgaatgccg atggcggata
    62941 acctttggtg gcgggcggtt cgccgagcga gagggcgatt tcccgctgcg ccatcgcgta
    63001 gcgggtcagg gaatccacca gcagtaagac gtgatgcccg cggtcgcgaa accaggcggc
    63061 gatggagtgg cacagttcgg tggctttcag acgcatcagc ggcgattcat cggcaggcgc
    63121 gacgacgacg atagactttg ccagcccgtc cgcgcccagc gagtggtcga taaactcttt
    63181 cacttcgcgc ccgcgttccc caatcagccc aaccaccacg atatcggctt tggtctggcg
    63241 ggtgatcatc cccagcagca cgcttttccc gacgccgctg cccgccatta gtccgacgcg
    63301 ctggcctttg ccgatggtca gcaggccgtt gatcgctttc acgcccacgt ccagcggttc
    63361 gtctaccgaa cgacgagtta agggattaac cgagggcggc aacggcggca gaaggtcgtt
    63421 gccgttcagg cggcctttgc catccagcgg ttcgccgagg ccattcacca cgcggcctaa
    63481 ccagctttcg cctatcagga tgtcgtgggc tttttcttcc gggaagacgc gtgcgcctgc
    63541 catcaggccg accggctgtt taaacggcat taaataggtg atgtcgcgat taaagcccac
    63601 ggcctgcgcg tcgatcagcg tgtgattggc actttcgatg cggcacagct gcccggtcat
    63661 tagcgggcag ccgacactct ccagcaggat gccgttaagg cgcaccagcc gcccggcgac
    63721 gcgggccagc gggatcgact ccagcgagcg cagggcgtta tcgaacgcag aaaaatcact
    63781 caccctgcga ctccggcgtg agtgtttctt tcagcgcggt catgcattgt tcaaggcgat
    63841 gctcgcagcc gatatccagt tcggatttgt cggtgatcac ccggcattcg cccggtggca
    63901 aatccggcga aggcgtcagc ccccattcgc tgaccttttc cggcaccgcg tcgcgcaagc
    63961 ggttaaactc ttccgtgctc agcattactt gcagggtttc cggcatcgcc gggaaggccg
    64021 ccagcgcctc ttccaccagc gccagcaact gcgtgggttg cagcgccagt tcacaacgga
    64081 tcacctggcg ggtgactttt tccaccagtt gcagcaaatc ttcccgctgt ttacgttcga
    64141 tatgagcgag gaagtcgttc actttaccgg tgatggcttc cagcggctgc gccgcctcaa
    64201 caaactgttt gcgcccttcc tgctgcccgg cgaggctgcc ttcggtgtaa ccctggcgtc
    64261 gcccctcggc aaaaccttgc tgatgacctt cgctaaagcc ctgctcctgg ccttcggtca
    64321 ttccctgagc aaaccctttt tgcagccctt cctgaaatcc gtccatcaac tggcgctggt
    64381 aatcggcggc gctaatactg gtagtgagcg gttctccggt caccgcacgc tggcgcggcg
    64441 ggaaacggtg caggcgatag cgcccgcgca gtgtttcaat ggtcatggtt actccgccgt
    64501 ctgttccgca aagagctgta gctgaatttc gcccgcctct tccagttcac gggcgatgcc
    64561 catgatttcg cggcggatct gttcgatacg gctgaccggc accggcccca gacgcgcggt
    64621 gatggcttcc agttgctgcg cctgccgttt tggcattacc gcgtaaatcg aacggcgcag
    64681 cagggcttcg gtgcctttca gcgccaccgc ccagtcttcc atcggcactt cgtcaagcag
    64741 acgtcgacgc acctcttcgt tctggcggct gaggataaag aagtcgtaca tctcatcctg
    64801 gagctgctcc agcacctctt cgtcgcgctc gcgcatctgg tcgaggatca cctgctggtt
    64861 gccctggaag cggttgacga tatccgccgc ctgtttaatg cctttcacct tcgagccatg
    64921 ttccgaaagt accgacaggc cacgttcgat cagcctgtcc agttcatcca ccacgtcgcg
    64981 gttaacgtcg ttaagtttcg ccacccggta gagaatttcg ttttgcaccg attcattgag
    65041 atacgagagc accgccgccg agatttccgg cgtcagaaac gcgaggaaca ccgcctgtaa
    65101 ttgcaaatgc tcttcggaga tcagcatcgc cagctggcgt ggttccaccc attgcaggcg
    65161 cgccatccgc gagcggatct catcgccgta gatgccgttg atcacgctgc tggcgatctc
    65221 ggtgcctagc gctttgttga gaataccctg caacatggat cgcgatgcgc cgttgatccc
    65281 gctctgttcg cggaactcat caaagaagtt attgatcact ttgcgcgcca tgctggtttt
    65341 cacgccggaa agccgcgcca tgttttcgct caggcgcact acttcttcgc ggctgagttt
    65401 ttgcatcacc gtcgccgccg cctcttcgcc cagacacagc agcaggatcg ccgcctgttc
    65461 caggtagctg ttgctcttat ttgttgtcaa ttcgctcatt gctggtgatc cattgtctga
    65521 gaacttcggc tacgcgatcg gtgtcgctca tggccagttt ttgcaggaac tcgagtttta
    65581 cttccaggcc ggaactctga gacggcaggc tgtcatcgcc gggcagcgac ggcagatcga
    65641 cgtttttgcg ctcctcttcc ggcatggcaa tccacggttc gctgacggct tccgattctg
    65701 gtgtgataat cggtgcacgt accgccgtca gccgtttcat taccgggcgc accacgaaga
    65761 acagcaacaa cagcgccagc aggccgcagc caatcaggcg cacccaggcc agcacgctgt
    65821 catctttcca cagcggaata attggttcga ccggcaccgc ctgcggcaca aagttaagca
    65881 gtgaaagcgt caggttatcc ccacgttgcg cgtcaatacc ggcggcattg ttcagcaacg
    65941 cggtaagctg cgcggtctgc tccggcttcc agtttttcag cgccggagcg ttttgattga
    66001 gcaccaccgc cacattgagg cgtttcaccg caaagccggg atgctgaata tgctggacgc
    66061 tacggtcgta agagtaatcg cgcttgcttt cgctgtgttt ggataacgct tccggcgagc
    66121 gattttcttc cgtaccgtta gtcatttgat tcgccgcaac cggcggacgg ttgctgagag
    66181 agccgggcac acccatcgcc acctgattgg tgtcgctgtc cagcacgctc tcttcgcggt
    66241 ttattttcgg cgcgtcgccg tagtgttcct gagtttcgtc gatggtgctg aggtcgagat
    66301 ccggcatcac gctgacacgg taattcccgc tgccgaccag cgaatccagc acgttcgcca
    66361 cgctggcgtg ggttttgtcc tgaatgtctt tgaggatctg atcgcgttta cgggtagcgg
    66421 cagagaccgc ctcgcccgcg ccgatgccgt cggtcagcag atttcccgcc tgatcaacca
    66481 cgctaacttt gctggcgtgc agccccggaa tgctgccgga aaccagatgc acaatggcgt
    66541 tcacctggtc catattcagc ttcgcgccgt agtgcagacg caccaccacc gaggcgctgt
    66601 tttgcggctc atcgctgacc acaaacgagc tttctccgtt gagcgccaga tgcacccgcg
    66661 cgctctccac cgcgtccagc gacataatgc tctgcgccag ctccccttcg aggctgcgtt
    66721 tatagcgcac gttctgcacg aactggctgc tgccaagcac ttcgtctttg tccatcagct
    66781 cgtagccgct gggcaaaatc gcctgcacgc ctttggcggc aagggtcatg cgcgttttcg
    66841 acagcgcctc ttccggcacc agtatctgcc cgctttgcgg gtcaatgcgg taggtgagct
    66901 tttcgccgtc cagcacggtg acaatctgcg acacgggcag attttcctgg cgtccgtaga
    66961 gcgagacgta gccgcgattg ccggtccaca gcacgctaac gataatcgcc gtcgccgcca
    67021 cggcaagccc cacacccgcc atcagcgccc agcgtttgtt atctgcgaga cgcagtcgaa
    67081 acgccggaaa cgcctgggtt aacttcttta tttgtgcatt catagggtta gatcgacata
    67141 ctcatcagat cgttaaatcc ggtggcgatt ttgttgcgca cctgcaccag cgcggagaaa
    67201 gagagcgacg cctgctggct ggcgatcatc gcacctgcca gatcatcgct tttgcccatg
    67261 tcgatagccg tttgctgctg ctccgccacc tgctggaact ggtcaacgtg accgagcgcg
    67321 ccctgcataa tgcggtgaaa cgaaacgtcc gggtcggtgg aactgaaggt catggcgggc
    67381 agcaccggcg cctgggcgtt ggcctgcatc cgctgcacgt cctgcatgat ctgcgcctgc
    67441 atggtgctgt tgattgtggt aatgctcatt tttaacttcc gggtcaggag aaaacttcga
    67501 taccgtgctt gcgcatggag gccagtcgat agcgcagtgc gcgcggggta atacctaaca
    67561 ggtcggcgat tttgctgcgg ttgccctgat atttacgcat caggtcagcg atatattgat
    67621 actgggcgct tcgtccgtgc tggcctaaat cgctggtttc ggcaatgtgt acggcgggct
    67681 gagcaggcca ttgcagttcg ctgtggtcgg caatgtcatt ttccggcagg cctaaggcgt
    67741 ccgggtaaat cacgccgtcg cgatttaaaa tcattccccg ctgaatggcg ttctccagct
    67801 ggcgcacatt gccgggccag cggtaattca gcatcgcacg gcgggtagat tccgaaaggg
    67861 tgatattttt caccagcacc gtggagtatt tttttataaa tgactctgcc agcggaataa
    67921 tatcgttcag acgttcacgc agcggcggca tggtaatagg aataaccgcc agacgataat
    67981 agagatcttc acgaaaacgc ccggcggcga cttcctgctc aaggttttta ttggtgcagg
    68041 caatcaggcg aaaattgagt ttaatttgtc ggttgctgcc taatcgctca acctgttgtt
    68101 cctgcaatac gcgtaatatt ttggcctgta acgccagcgg catatcgcca atttcatcga
    68161 gtaataaggt gccgttattc gccagttcca tttttcctgg cacactggca attgcgccgg
    68221 taaatgcgcc tttgtcgtag ccaaataagg ttgcttccag catgttttcc gggatcgcag
    68281 cgcagttcac gccgatatag ggcgcgtttt cattttcacc aaaggcgact gtgtgaatat
    68341 atttcgccac gcactctttg cccgcgccgg tttcgccctg aatgagcacc ggcacgttaa
    68401 atgcggcgac acgctttgcc agagtaaaag cgttaatgct ggaagcggca gttgcaataa
    68461 gttcagacat agggtgaacg atataagtag cgacaataat caggatgttc tgaagcgtat
    68521 caccggcggg attaacaagt tcgaattaaa cgataataaa aatattattt gtttataaac
    68581 aatgatttat ccatgtaggc cggataaggc gtttacgccg catccggcaa ttgtgcaccg
    68641 gtgcctgatg cgatgcggcg cgtcttatca ggcctacggt tcgtgcaatg tgttatgacg
    68701 atgcctgata tctcgccctt taataattta attatttttt caccctatta ataatttgcc
    68761 cctttcacgc cctctctatt taatacctca ccttgtgtca ctgccggatt tgcgtgcaca
    68821 aaatcgccca taccattaca gcagagaaag taagccatgc tgaagtacag taaaacgcca
    68881 ggcatcttta aattagaagg taatcggctc ggacgcccct accatcgcct gccgaccctg
    68941 tttacgggga attttgacgt gattgattcg catctgggca gctactttct caaaaagcat
    69001 cgcagcaata tcaccttaaa aaagattggc tgcgaaatgg atattattaa taaaaacgct
    69061 gaactgatgg tttcccaggt cgggcatctg gcgtttgata ttgatcgctc tttgctgtta
    69121 atgctgctgg gcaattttta tggtctggag tcctctcttg aagaggcgaa agcgcataac
    69181 gggctgccga ccaaaaccga aacgcggtta aaaaaccgtc tggcgctgga tatctgcacg
    69241 ctgattttta atctgcaaac ctccggtatt gcgctgaaat taaagctgga cagcagcacg
    69301 gtaattaccc actgggcgta tcaattaacc tttacccttg cgggcgatga agagagctgt
    69361 tttcgtattt tgctcgacga cgcccacacc gattttattt taaatctgat ccgccacagc
    69421 gagcacagta aaccgcagca ggtggcgaaa tcggtcaata aaccggccct gattaaagag
    69481 atcatccgct ccctgccgct gaccctgaac gtcaaaatcg ccgagctatc gatgaatgtc
    69541 gcggatctca cgcagataaa agccggggac attttaccca tctcgctggg agaaacgttc
    69601 ccggtggcga ttgggcaatc tgaattattt agcgccctga ttgtggaaga caaagacaaa
    69661 ctgtttttgt cggagctggc gggcaaaaat gagaatagcc atgagtaagc aagaagatat
    69721 tttagccggg gattttggcc tcaccgatga agttgcgcct gtcgcaaaat ccgccgacgc
    69781 tgaaacgctg gtcacccgcc tggaagaccg tttttccgac tcgatgacgc tgctaaaacg
    69841 cattccggtg accttaacgc tggaagtgtc atcagtagaa ataatgttag ccgacctcct
    69901 taacatcgac gacgacacgg tgattgagct ggataaactc gccggggagc cgctggatat
    69961 caaagtgaac aatatcctgc tgggcaaagc ggaagtggtg gtggtcaacg agaagtacgg
    70021 cctgcgcgtg ctggaattta acacccgcga tatcaacgac ctggcaccat gaaacgccgc
    70081 actcaactga cgctgggcct tgggctgctg gcgcttgcgc cggtggcgtt ggcccagggc
    70141 ggcgatattg cgctgcttaa cgtggtcacc cacggcaaca ctcaggagta cagcgtcaaa
    70201 atccaggtgc tgattctgat gaccctcgtc ggtttgctgc ctacgatggt gctgatgatg
    70261 acctgcttta cgcgctttat tatcgtgctg tcgctgctgc gccaggcgct agggctacaa
    70321 caaacgccgc caaaccgcat tctgattggc attgcgctct ccctcaccat gctggtgatg
    70381 cgccccatct ggctcaatat ttacgaccac gcagtagtgc cgtttgaaaa tgatcagatt
    70441 accctgaccg atgcgttaag caccgccgcg acgccactca aacgctttat gctggcgcag
    70501 actgacaaaa aagcgatggc gcaaatcatg actatcggcg gcgcgaaggg ccatgccgcc
    70561 gaccaggatc tctcgattgt ggtgcccgcc tatgtgttga gcgagctgaa aaccgccttc
    70621 cagattggct ttatgattta catcccgttt ctggttatcg acctgattgt cgccagcgta
    70681 ctgatggcga tggggatgat gatgctgtcg ccgctgatcg tttcgctacc ctttaagctg
    70741 atgctgtttg tgctgatcga cggctggtcg ctgaccatcg gtacactcac caccagcatt
    70801 cgcgggctgg ggctgggctg atgatgctga ccatagacgt agccgccgac attgtcgcca
    70861 gcggcataaa agtggtgatt ttgctggttt cggtgctggt agtgccgagc ctgctggtcg
    70921 gcctgctggt gagcgtgttc caggcggtaa cgcagattaa cgaacagacc ctaagctttt
    70981 tgccgaggct gattgtcacg ctggtggtgc tgggcgtgtg cggtaaatgg atgattatcc
    71041 agttgcatga tctctgcatt cacctgttta cccaggccgc gttgctggtt cattaagatg
    71101 cgcacaagcg acgttacgca actgatggat ctggcgctgg ggctgtggtt tccctttgtg
    71161 cgcattatgg cgtttctgcg ctatgtgcct gtgctggata acagcgccct gaccgtgcgc
    71221 gtgcggatta ttttgtcgct ggcgctggca atcatcatca cgccgttgat cccccatcct
    71281 gttccccacg acctgatatc gctcaacagc ctgatcctga cggtggagca aattttatgg
    71341 ggcatgttat tcgggctgat gtttcagttt ttgtttctgg cgctacagct ggccgggcaa
    71401 attctctcgt tcaatatggg gatgagcatg gcggtgatga acgacccgag cagtggcgca
    71461 tcgaccaccg tgctggcgga gctcatcaac gtctacgcga tcctgctgtt ttttgccatg
    71521 gacgggcatc tgctgctggt gagtgtgctg tataagggct ttacctactg gccgattggc
    71581 aacgcgctgc atccgcaaac cctgcgcacc atcgcgctgg cctttagctg ggtgctggcg
    71641 tccgcatcgc tgctggcgct gccaaccact tttatcatgc tgattgtgca gggctgcttt
    71701 ggcctgctca accgcattgc gccgccgctg aacctttttt cactcggttt tcccatcaac
    71761 atgctggcgg ggctggtctg ctttgccacc ctgctctaca acctgccgga tcactatctg
    71821 catctggcga attttgtgtt gcagcaactc gacgcgctga aaggtcacta tggcggatag
    71881 cagcagcgaa gaaaaaacag agaaaccctc ggcgcaaaag ctgcgtaaag cgcgcgaaga
    71941 ggggcaactg ccgcgctcga aggatatggg gctggcggcg agcctgtttg cggcatttgt
    72001 ggtgatctcc agcagcttcc cgtggtatgc cgattttgtg cgcgagagtt ttatcagcgt
    72061 gcaccagtat gcacaggaga ttaataaccc agaggtgatc ggccagtttc tgcgccatca
    72121 cctgttaatt ctcggcaagt ttattctcac cttgctgccg atgcccgccg ccgcgctact
    72181 ctcctcacta gtgcccggcg gctggctgtt tctgcctaaa aaaatcctcc cggatttcag
    72241 caaaatcagc ccgttaaaag gcattaggcg gctgttttcc agtgagcatc tggcggaaac
    72301 gggaaagatg acggtgaagt cggtggtggt gctggtgatg ctgtgggtaa gcctgcgcaa
    72361 taactttgcc gcctttctcg gcctccaggc gctaccgttc aaactggcga tgaatgaggg
    72421 tttatcgctc tacgcctgcg tgatgcgcaa ctttgtcatc ctgtttatct tctttgcgtt
    72481 aatcgacgtg ccgctggcga aagccctgtt taccaaaggg ctgaagatga ccaaacagga
    72541 gctgaaagaa gagtataaaa accaggaagg caagccggaa gtgaaggcgc gcgtgcggcg
    72601 tctgcaacgg cagttggcga tggggcaaat tcgcaaagtg gtgccgaaag ccaacgtggt
    72661 gatcaccaac ccgacccact acgccgtggc gttgcagtat gactcgtcgc gcgccgccgc
    72721 gccgtttgtg gtggcaaaag gcaccgatga aatcgccctc tatattcgcc aggtcgccgc
    72781 tgagaatcag gtggaagtgg tcgagttccc gcgactggcg cgctcggttt actacaccac
    72841 ccaggtgaac cagcaaattc cgtttcagct ttacagggcc atcgcccacg ttctgaccta
    72901 cgtcctgcaa atgaaacact ggcgcgaggg tgcacagccg cgcccggccc tgaacagaca
    72961 tatttccatt cccaaagagg tgcttaaact ggatggcgaa aacaactaaa tcgttcctcg
    73021 cgctgctgcg cggcggcaat cttggcgtgc cgctggtgat actgtgtatt ctggcgatgg
    73081 ttattttgcc gctgccgcct gcgctgctgg atattctgtt caccttcaac attgtgctgg
    73141 cggtgatggt gctgctggtg gcggtgtcgg cgaaacgacc gctggaattc agcctgttcc
    73201 cgaccatttt gctgatcacc accttaatgc gcctgacgct caacgtcgcc tctacgcgcg
    73261 tggtgctgct gcacgggcat ctcggcgcgg gcgcggcggg taaggtgatt gagtcatttg
    73321 gtcaggtggt gattggcggc aactttgtcg tcggtttcgt ggtgtttatc atcctgatga
    73381 tcatcaactt tattgtcgtc accaaagggg ccgagcgtat ctccgaggtt tctgcccgct
    73441 ttaccctgga cgcgatgccc ggtaagcaga tggcgattga cgccgatctt aatgccgggt
    73501 tgatcaatca ggcgcaggca caaacccgac gtaaagatgt tgccagcgag gccgatttct
    73561 acggcgcgat ggacggggca tcgaaatttg tgcgcgggga tgctatcgcc gggatgatga
    73621 ttctggcgat caacctgatc ggcggcgtct gtatcgggat cttcaaatac aacctgagcg
    73681 ccgacgccgc cttccagcag tatgtgctga tgaccatcgg cgacgggctg gtggcgcaaa
    73741 tcccttccct gctactctcc accgcggcgg cgattatcgt cacccgcgtc agcgacaacg
    73801 gcgatatcgc ccatgacgtg cgtaaccagt tgctggcaag cccatcggtg ctctacaccg
    73861 ctaccggaat tatgttcgta ctggcggtag tgccgggaat gccacatctg ccgtttttgc
    73921 tgttcagcgc cctgcttggc tttaccggct ggcggatgag caaacgcccg caggcggcgg
    73981 aagcggaaga gaaaagtctc gaaacgctga cccgcaccat caccgaaacc agcgagcaac
    74041 aggtcagttg ggaaaccatt ccgctgatcg agcccatcag cttaagcctc ggctacaagc
    74101 tggtggcgct ggtggacaaa gcccagggca acccgctcac ccagcgtatt cgcggcgtgc
    74161 gacaggtgat atccgacggc aacggcgtgc tgctgccgga gatccgcatt cgggaaaact
    74221 tccgccttaa gcccagccag tacgctattt tcatcaacgg cattaaggct gatgaagcgg
    74281 atattccggc ggataaactg atggcgctgc cctccagcga aacctacggc gagatagacg
    74341 gcgtgctggg gaatgacccg gcgtacggga tgccggtgac ctggatccag cctgcgcaga
    74401 aagcgaaggc gctgaatatg gggtatcagg tgatcgacag cgccagcgtg attgctaccc
    74461 atgtaaataa gattgtgcgc agctatattc ctgatttatt taactatgac gatattacgc
    74521 agttgcataa ccgtttggcg tcgatggcac cgcgcctggc ggaggattta agcgcggtgc
    74581 tcaattacag ccagttgctg aaagtgtacc gggcgctgct gaccgaaggc gtttccctgc
    74641 gcgatatcgt caccatcgcc accgtgctgg tcgccagtag cgcggtgacc aaagatcata
    74701 ttctgctggc agccgatgtg cgcctggcgc tgcggcgcag cattacccat ccgttcgttc
    74761 gcaagcagga gctgacggtg tataccctga ataatgagct ggaaaatctg ctgactaacc
    74821 tagtgaatca ggcgcaacag ggcggtaaag tgatgctcga cagcgtgcca gtggacccga
    74881 atatgctcaa ccagttccag agtacgatgc cacaggtgaa agagcagatg aaagcggcgg
    74941 ggaaagaccc ggtgctgctg gtgccaccgc agctgcgccc tttgctggcg cgttatgcaa
    75001 ggttgtttgc gccggggctg catgtgctgt cgtataacga agtgccggat gagctggagt
    75061 tgaagattat gggggcgttg agttgatggg ggattctttt ccatgctttg cagcacctga
    75121 caccattaat tgacgtcaca ctgcaaagag actacgatga caccatacaa tgacgtcatg
    75181 gattgatagt atgattaagg tcagcgcgct aggttctcgt catagaaaaa cattcgacga
    75241 tgtaatgacg gagccgccaa aatctggaat taaatgggat gatgttgttg cactcattaa
    75301 agctgtgggt ggaacaataa aaaataacaa cggctccaga cgaaaatttc agattggaac
    75361 gaccaaattc agcacacatg agccacatcc aaaaaatgtg atggataaag gcgcagtagc
    75421 aggtcttaag gagtggtttg ttaactgtgt aggggtagat tatgaataaa gcatcgaaca
    75481 ttatcacaat taaaggtcaa ccagccgcag taacatttga agccgatatc aacgcatttc
    75541 gcggcaagtt cctcaatgta aacggttatt gtgactttgt agccacaagt attgaggcat
    75601 tatatcgtga aggcgaaagc gcgctggatg agtggatggc ggattgtgaa gaggacggta
    75661 ttgccccttt tcgtgaagaa gaagagcaaa aacgcctgac gcttcgtgtt cctcatcaca
    75721 ttgattcgcg gctgaccatc gtcgccagac aacattccat ttcaaaaaac cagctcattg
    75781 ttgatgtctt agaacgtgag ttttcagccc agatgtaaac aggtcggatg ccgcgtggca
    75841 tccgaccttt tgccagttcg tcaatattac ccatttacaa aatcgtctcc tccgtcaata
    75901 tccgccgcgc gcctaaaaag tgatcctgcc agaaaggttc agctaaccga cttacccgaa
    75961 tggtttcccc ggtgcgcggt gactcaataa actgaccgtc gcccagatac acccccatat
    76021 ggtcggcaat ctcgcggctg tggatatgga aaaatagcaa atctccccgg cgcaggtcgt
    76081 tattcgccac aatggttgcc cggcgatagt gatacatctc attagccgtg cgtgggagtt
    76141 tcgcctcaag gatcttgttg taggcataaa aaactaaccc gctacagtcg aagccttgat
    76201 cgggccgcgt accgccccag acgtaaggct tgccaagctg ttgctccaga cggtgaatcg
    76261 ctacttcggt gatgttatgc agatggtcgc tgcttaagaa atggttgttt tccgccagcg
    76321 cctgccagcg tctgtcactc gccttgagcg gcgcaggaaa ccattctgga tgctgtttta
    76381 tctgttcgcg gttgtgctgg cgtagcgccc ttctgctttc ggcattgcct tccacaatat
    76441 agctggcctg ttttttcaga tgggtttggt actgttttaa taaacgcgca cgattttgca
    76501 tccgctggcg ggcggcgtag ctgaaagtga tatgtgatgt ggtggcgctg gcaaatgaag
    76561 aaaacggcaa gcaaagcaac agtaatcccg acaacacaaa agagggaatc gacggcaagg
    76621 acatactcaa taatggctga tcatgcattg atgttgcagt gtatcccggc ccgtacctga
    76681 acaaacttaa taaaccatta ttataaaatt aataaatata tttcagttaa ttatgtaaaa
    76741 acatccgttt attaaattac tcatggattg tcgctgttgc tcatttgagc tacaattcaa
    76801 gctgaataaa tatacagcac aggagatacc ccaatggctg ctaacgcatt tgttcgcgcc
    76861 cgaatcgatg aagatctgaa gaatcaggcg gcggacgtac tggccgggat ggggctgacc
    76921 atctctgacc tggttcgcat aaccctcaca aaggtcgcgc gtgaaaaggc attgccgttt
    76981 gatttacgcg agcctaatca attaaccatt caatcaatca aaaacagcga agctggcgtt
    77041 gatgttcata aggccaaaga cgccgatgat ttatttgata aattaggaat ttaaatatga
    77101 ttcaaaggga tattgaatac tcgggacaat tttcaaagga tgtaaaagtt gcacaaaagc
    77161 gtcataagga tatgaataaa ttgaaatatc ttatgacgct tcttatcaat aatactttac
    77221 cgcttccagc tgtttataaa gaccacccgc tgcaaggttc atggaaaggt tatcgcgatg
    77281 ctcatgtcga accggactgg atcctgattt acaaacttac cgataaactt ttacgatttg
    77341 agagaactgg aactcacgcg gcgctctttg ggtaaagcac gctgccgatg aaatttgtaa
    77401 accctgcgcc gacaggcttg cgta
//
LOCUS       DAEVZA010000006        67477 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEVZA010000006 DAEVZA010000000
VERSION     DAEVZA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.006"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..939
                     /locus_tag="LAF92_000433"
     CDS             <1..939
                     /locus_tag="LAF92_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI9789494.1"
                     /translation="NDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSLNLSI
                     PLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHNSSLN
                     ASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASG
                     VRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMV
                     AARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISSKSQS
                     WTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            950..2041
                     /locus_tag="LAF92_000434"
     CDS             950..2041
                     /locus_tag="LAF92_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9789495.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(2084..2944)
                     /gene="rsmI"
                     /locus_tag="LAF92_000435"
     CDS             complement(2084..2944)
                     /gene="rsmI"
                     /locus_tag="LAF92_000435"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBI9789496.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            3008..5044
                     /gene="lpoA"
                     /locus_tag="LAF92_000436"
     CDS             3008..5044
                     /gene="lpoA"
                     /locus_tag="LAF92_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBI9789497.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            5002..5397
                     /locus_tag="LAF92_000437"
     CDS             5002..5397
                     /locus_tag="LAF92_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBI9789498.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTIFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5417..6007
                     /gene="diaA"
                     /locus_tag="LAF92_000438"
     CDS             5417..6007
                     /gene="diaA"
                     /locus_tag="LAF92_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBI9789499.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            6017..6592
                     /gene="yraP"
                     /locus_tag="LAF92_000439"
     CDS             6017..6592
                     /gene="yraP"
                     /locus_tag="LAF92_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBI9789500.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6706..7746)
                     /locus_tag="LAF92_000440"
     CDS             complement(6706..7746)
                     /locus_tag="LAF92_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI9789501.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7819..8454)
                     /locus_tag="LAF92_000441"
     CDS             complement(7819..8454)
                     /locus_tag="LAF92_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBI9789502.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            8582..9100
                     /gene="yhbO"
                     /locus_tag="LAF92_000442"
     CDS             8582..9100
                     /gene="yhbO"
                     /locus_tag="LAF92_000442"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBI9789503.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9080..9523)
                     /locus_tag="LAF92_000443"
     CDS             complement(9080..9523)
                     /locus_tag="LAF92_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBI9789504.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            9574..9876
                     /gene="yhbQ"
                     /locus_tag="LAF92_000444"
     CDS             9574..9876
                     /gene="yhbQ"
                     /locus_tag="LAF92_000444"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBI9789505.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAESAGFAELLSSLQTPEIKSD"
     gene            complement(9863..10366)
                     /locus_tag="LAF92_000445"
     CDS             complement(9863..10366)
                     /locus_tag="LAF92_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI9789506.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10360..10884)
                     /locus_tag="LAF92_000446"
     CDS             complement(10360..10884)
                     /locus_tag="LAF92_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBI9789507.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11093..12088
                     /locus_tag="LAF92_000447"
     CDS             11093..12088
                     /locus_tag="LAF92_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBI9789508.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12097..12975
                     /locus_tag="LAF92_000448"
     CDS             12097..12975
                     /locus_tag="LAF92_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBI9789509.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAICSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13181..14188
                     /locus_tag="LAF92_000449"
     CDS             13181..14188
                     /locus_tag="LAF92_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HBI9789510.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14306..15550)
                     /gene="mtr"
                     /locus_tag="LAF92_000450"
     CDS             complement(14306..15550)
                     /gene="mtr"
                     /locus_tag="LAF92_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBI9789511.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15704..17593)
                     /gene="deaD"
                     /locus_tag="LAF92_000451"
     CDS             complement(15704..17593)
                     /gene="deaD"
                     /locus_tag="LAF92_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBI9789512.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17586..17666)
                     /gene="yrbN"
                     /locus_tag="LAF92_000452"
     CDS             complement(17586..17666)
                     /gene="yrbN"
                     /locus_tag="LAF92_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBI9789513.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17773..18657)
                     /gene="nlpI"
                     /locus_tag="LAF92_000453"
     CDS             complement(17773..18657)
                     /gene="nlpI"
                     /locus_tag="LAF92_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBI9789514.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18766..20901)
                     /gene="pnp"
                     /locus_tag="LAF92_000454"
     CDS             complement(18766..20901)
                     /gene="pnp"
                     /locus_tag="LAF92_000454"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBI9789515.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21148..21417)
                     /gene="rpsO"
                     /locus_tag="LAF92_000455"
     CDS             complement(21148..21417)
                     /gene="rpsO"
                     /locus_tag="LAF92_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBI9789516.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21566..22510)
                     /gene="truB"
                     /locus_tag="LAF92_000456"
     CDS             complement(21566..22510)
                     /gene="truB"
                     /locus_tag="LAF92_000456"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBI9789517.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22510..22911)
                     /gene="rbfA"
                     /locus_tag="LAF92_000457"
     CDS             complement(22510..22911)
                     /gene="rbfA"
                     /locus_tag="LAF92_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBI9789518.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23075..25747)
                     /gene="infB"
                     /locus_tag="LAF92_000458"
     CDS             complement(23075..25747)
                     /gene="infB"
                     /locus_tag="LAF92_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBI9789519.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25772..27259)
                     /gene="nusA"
                     /locus_tag="LAF92_000459"
     CDS             complement(25772..27259)
                     /gene="nusA"
                     /locus_tag="LAF92_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBI9789520.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(27287..27739)
                     /gene="rimP"
                     /locus_tag="LAF92_000460"
     CDS             complement(27287..27739)
                     /gene="rimP"
                     /locus_tag="LAF92_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBI9789521.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27946..28022)
                     /locus_tag="LAF92_000461"
     tRNA            complement(27946..28022)
                     /locus_tag="LAF92_000461"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27986..27988),aa:Met,seq:cat)
     gene            28370..29713
                     /gene="argG"
                     /locus_tag="LAF92_000462"
     CDS             28370..29713
                     /gene="argG"
                     /locus_tag="LAF92_000462"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBI9789522.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKVK"
     gene            29897..30850
                     /locus_tag="LAF92_000463"
     CDS             29897..30850
                     /locus_tag="LAF92_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9789523.1"
                     /translation="MNGEYFVRLALHTLKCTQKDLASHLGVSSTQISKWKKGEHMSAD
                     MEKKFRNITQIGDYSPQLVEWTGSVANAEKWDRLIHFLARRAMDDAETGYITQLLTDE
                     DGFLVEETIDVLKRIGFPTPLSFPEGLNIDDDNADEEEAFWEILESNAHCSVINDIYH
                     ALNDVYGFYIAYVDELIQDDDLDVYSSEAINIQSSLISLAACKIEIDTPVASNFKEFR
                     YRVKKDYENWLNQLKMMAFRAGIPLRAELLEMVYNTADQLSVAAEAERFDFNKSRIHP
                     DIYMNEILTGMRIIHQVLPVIMQKLEITDFKLDETDLCLGK"
     gene            complement(30925..31011)
                     /locus_tag="LAF92_000464"
     tRNA            complement(30925..31011)
                     /locus_tag="LAF92_000464"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30975..30977),aa:Leu,seq:gag)
     gene            complement(31026..31358)
                     /gene="secG"
                     /locus_tag="LAF92_000465"
     CDS             complement(31026..31358)
                     /gene="secG"
                     /locus_tag="LAF92_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBI9789524.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(31586..32923)
                     /gene="glmM"
                     /locus_tag="LAF92_000466"
     CDS             complement(31586..32923)
                     /gene="glmM"
                     /locus_tag="LAF92_000466"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBI9789525.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32916..33764)
                     /gene="folP"
                     /locus_tag="LAF92_000467"
     CDS             complement(32916..33764)
                     /gene="folP"
                     /locus_tag="LAF92_000467"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBI9789526.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(33854..35788)
                     /gene="ftsH"
                     /locus_tag="LAF92_000468"
     CDS             complement(33854..35788)
                     /gene="ftsH"
                     /locus_tag="LAF92_000468"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBI9789527.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35888..36517)
                     /gene="rlmE"
                     /locus_tag="LAF92_000469"
     CDS             complement(35888..36517)
                     /gene="rlmE"
                     /locus_tag="LAF92_000469"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBI9789528.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            36643..36936
                     /gene="yhbY"
                     /locus_tag="LAF92_000470"
     CDS             36643..36936
                     /gene="yhbY"
                     /locus_tag="LAF92_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBI9789529.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37092..37568)
                     /gene="greA"
                     /locus_tag="LAF92_000471"
     CDS             complement(37092..37568)
                     /gene="greA"
                     /locus_tag="LAF92_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBI9789530.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            37816..39249
                     /gene="dacB"
                     /locus_tag="LAF92_000472"
     CDS             37816..39249
                     /gene="dacB"
                     /locus_tag="LAF92_000472"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBI9789531.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(39289..40461)
                     /gene="cgtA"
                     /locus_tag="LAF92_000473"
     CDS             complement(39289..40461)
                     /gene="cgtA"
                     /locus_tag="LAF92_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBI9789532.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(40477..41442)
                     /locus_tag="LAF92_000474"
     CDS             complement(40477..41442)
                     /locus_tag="LAF92_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI9789533.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(41569..41826)
                     /gene="rpmA"
                     /locus_tag="LAF92_000475"
     CDS             complement(41569..41826)
                     /gene="rpmA"
                     /locus_tag="LAF92_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBI9789534.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(41847..42158)
                     /gene="rplU"
                     /locus_tag="LAF92_000476"
     CDS             complement(41847..42158)
                     /gene="rplU"
                     /locus_tag="LAF92_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBI9789535.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            42417..43388
                     /gene="ispB"
                     /locus_tag="LAF92_000477"
     CDS             42417..43388
                     /gene="ispB"
                     /locus_tag="LAF92_000477"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBI9789536.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            43616..43894
                     /gene="sfsB"
                     /locus_tag="LAF92_000478"
     CDS             43616..43894
                     /gene="sfsB"
                     /locus_tag="LAF92_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBI9789537.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43942..45201)
                     /gene="murA"
                     /locus_tag="LAF92_000479"
     CDS             complement(43942..45201)
                     /gene="murA"
                     /locus_tag="LAF92_000479"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBI9789538.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45256..45510)
                     /gene="ibaG"
                     /locus_tag="LAF92_000480"
     CDS             complement(45256..45510)
                     /gene="ibaG"
                     /locus_tag="LAF92_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBI9789539.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(45670..45963)
                     /gene="mlaB"
                     /locus_tag="LAF92_000481"
     CDS             complement(45670..45963)
                     /gene="mlaB"
                     /locus_tag="LAF92_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBI9789540.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45963..46598)
                     /gene="mlaC"
                     /locus_tag="LAF92_000482"
     CDS             complement(45963..46598)
                     /gene="mlaC"
                     /locus_tag="LAF92_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBI9789541.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(46617..47168)
                     /gene="mlaD"
                     /locus_tag="LAF92_000483"
     CDS             complement(46617..47168)
                     /gene="mlaD"
                     /locus_tag="LAF92_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBI9789542.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47173..47955)
                     /gene="mlaE"
                     /locus_tag="LAF92_000484"
     CDS             complement(47173..47955)
                     /gene="mlaE"
                     /locus_tag="LAF92_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBI9789543.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47963..48772)
                     /gene="mlaF"
                     /locus_tag="LAF92_000485"
     CDS             complement(47963..48772)
                     /gene="mlaF"
                     /locus_tag="LAF92_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBI9789544.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48982..49959
                     /locus_tag="LAF92_000486"
     CDS             48982..49959
                     /locus_tag="LAF92_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBI9789545.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49973..50959
                     /gene="kdsD"
                     /locus_tag="LAF92_000487"
     CDS             49973..50959
                     /gene="kdsD"
                     /locus_tag="LAF92_000487"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBI9789546.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50980..51546
                     /gene="kdsC"
                     /locus_tag="LAF92_000488"
     CDS             50980..51546
                     /gene="kdsC"
                     /locus_tag="LAF92_000488"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBI9789547.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            51543..52118
                     /gene="lptC"
                     /locus_tag="LAF92_000489"
     CDS             51543..52118
                     /gene="lptC"
                     /locus_tag="LAF92_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBI9789548.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52087..52644
                     /gene="lptA"
                     /locus_tag="LAF92_000490"
     CDS             52087..52644
                     /gene="lptA"
                     /locus_tag="LAF92_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBI9789549.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            52651..53376
                     /gene="lptB"
                     /locus_tag="LAF92_000491"
     CDS             52651..53376
                     /gene="lptB"
                     /locus_tag="LAF92_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBI9789550.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            53424..54857
                     /gene="rpoN"
                     /locus_tag="LAF92_000492"
     CDS             53424..54857
                     /gene="rpoN"
                     /locus_tag="LAF92_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBI9789551.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPIYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54880..55167
                     /gene="hpf"
                     /locus_tag="LAF92_000493"
     CDS             54880..55167
                     /gene="hpf"
                     /locus_tag="LAF92_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBI9789552.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55285..55776
                     /gene="ptsN"
                     /locus_tag="LAF92_000494"
     CDS             55285..55776
                     /gene="ptsN"
                     /locus_tag="LAF92_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBI9789553.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            55822..56676
                     /gene="rapZ"
                     /locus_tag="LAF92_000495"
     CDS             55822..56676
                     /gene="rapZ"
                     /locus_tag="LAF92_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBI9789554.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            56673..56945
                     /gene="npr"
                     /locus_tag="LAF92_000496"
     CDS             56673..56945
                     /gene="npr"
                     /locus_tag="LAF92_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBI9789555.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57159..57791
                     /gene="yrbL"
                     /locus_tag="LAF92_000497"
     CDS             57159..57791
                     /gene="yrbL"
                     /locus_tag="LAF92_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBI9789556.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(57788..58516)
                     /gene="mtgA"
                     /locus_tag="LAF92_000498"
     CDS             complement(57788..58516)
                     /gene="mtgA"
                     /locus_tag="LAF92_000498"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBI9789557.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(58513..59166)
                     /gene="elbB"
                     /locus_tag="LAF92_000499"
     CDS             complement(58513..59166)
                     /gene="elbB"
                     /locus_tag="LAF92_000499"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBI9789558.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(59396..61732)
                     /gene="arcB"
                     /locus_tag="LAF92_000500"
     CDS             complement(59396..61732)
                     /gene="arcB"
                     /locus_tag="LAF92_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBI9789559.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(61828..62757)
                     /locus_tag="LAF92_000501"
     CDS             complement(61828..62757)
                     /locus_tag="LAF92_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBI9789560.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            63432..>67477
                     /gene="gltB"
                     /locus_tag="LAF92_000502"
     CDS             63432..>67477
                     /gene="gltB"
                     /locus_tag="LAF92_000502"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBI9789561.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFG"
BASE COUNT        16476 a        18345 c        16710 g        15946 t
ORIGIN      
        1 aacgataaaa acaggaatat ctcagttggg gtttccggtc agcaatgggg aattggttac
       61 agcctgaatt atcaatacag tcgctacacg gatcaaaata atgaccgcgc actctctttg
      121 aatctcagta ttccgttaga acgctggtta ccgcgtagcc gggtttccta tcagatgacc
      181 agccagaaag atcgcccaac ccaacatgaa atgcgtcttg atggctcact gctggatgat
      241 ggtcgcctga gctatagcct ggaacaaagt ctggatgacg ataacaacca taacagtagc
      301 ctgaacgcca gttaccgttc accttatggc accttcagtg ccggatacag ctacggtaat
      361 gacagcagcc aatacaatta cggcgttacc ggcggcgtgg ttatccatcc tcatggcgtg
      421 acgctctcgc aatatctggg caacgctttt gcgcttatcg atgctaatgg ggcttctggc
      481 gtgaggatac aaaactatcc ggggattgct actgatccct ttggctatgc agtggttcct
      541 tatctcacga cttaccagga aaaccgtctc tcggtagata ctacgcagct gcccgataac
      601 gtcgatcttg agcaaacaac acagtttgtg gtgcccaaca gaggtgcaat ggtagcggcg
      661 cgtttcaacg ccaatatcgg ttatcgcgta cttgttacag tcagcgatcg caacggtaaa
      721 ccgttgccct ttggcgctct tgccagcaac gatgagacgg ggcaacaaag tatcgtcgat
      781 gagggcggca tactatatct ctctgggata tcgagtaaat cacaaagctg gactgtacgc
      841 tggggaaatc aggcagatca acaatgtcag tttgctttta gtacaccgga ttcagaacca
      901 acaacctctg tattacaagg cacggcgcag tgccattaag gataaaaaaa tgaaaagagc
      961 gcctcttata acaggacttt tgttgatatc cacatcctgc gcttatgcct cctcaggagg
     1021 gtgtggagct gacagcacta gcggtgcgac aaattacagc agtgtggttg atgatgttac
     1081 ggtgaaccag acagataacg tgacaggacg ggagtttacc tctgccacgc taagtagcac
     1141 taactggcaa tacgcctgta cctgctctgc gggtaaggca gttaaacttg tctatatggt
     1201 cagccctgta cttaccacca ctggacatca agcaggatat tacaaactca atgatagtct
     1261 ggatattaaa accacattac aggcgaatga cattccagga ctcgtgactg accagaccgt
     1321 ttctgttaac acccgattca cacagataaa aagcaacacc gtatattctg ctgcaaccca
     1381 aacgggtgtt tgccagggtg acacgtctcg ttatggaccc gttaatattg gtgcaaatac
     1441 cacctttacc ctgtatgtca ccaagccatt tctcggctcg atgaccattc cgaaaacgga
     1501 tattgccgtc attaaaggcg catgggtcga tggaatggga agcccgtcta caggtgactt
     1561 ccatgattta gtcaagttat cgattcaggg aaatctcacc gccccacagt cgtgcaaaat
     1621 taatcagggc gatgttatta aggttaattt tggattcatc aatggtcaga agtttaccac
     1681 ccgcaatgcc atgccagacg gttttactcc agtagacttt gatatcactt atgactgtgg
     1741 tgatacttca aagattaaaa actcgttgca aatgcgcatc gacggtacaa ctggggtagt
     1801 agaccagtac aacctggtcg ccagacgaag aagttcagac aatgcgcccg atgtcggtat
     1861 tcgtattgaa aatctcggcg gcggagttgc aaatattcct tttcagaacg gtatccttcc
     1921 cgttgatcct tccgggcatg gcaccatcaa catgcgcgcc tggccagtta atctggtcgg
     1981 tggtgagctg gaaacaggaa aatttcaggg caccgccacc attaccgtca tcgtgcggta
     2041 aaagaagacg ttgcgttttg attaacgcaa cgcttcggca ctgttacccc tgctgctcca
     2101 gcgcatactt atacagcgca tttttcttca cgccgtgaat ttctgccgcc agcgccgccg
     2161 cttttttcaa cggcagttct gcctgtagca gcgccagcgt gcgcagggca tcggcgggta
     2221 agtcttcttc ctgtgcttta tgaccttcga taatcagcac catttcgcct ttgcgacggt
     2281 tttcatcttc ttttacccac gccagcagct cgccaacggg cgcgccgtga atggtttccc
     2341 aggttttggt cagctcacgc gccagaacca cgtagcggga ttcgcctaat accgcaacga
     2401 tatcttccag gctatctaac agacggtggg tggattcata aaaaatcagc gtgcgcggct
     2461 ccgcttcaat ggcttttagc gcatcacggc ggccttttga tttggcaggt aaaaagcctt
     2521 cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa cgcagtgata gcagcacacg
     2581 gcccgggtag cggcaccacg caaatccccg cttcacggca ggtacgcacc agatggtagc
     2641 caggatcgtt aattagcggc gttccggcat cggaaaccag cgcaatgttc tgcccttctt
     2701 gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc agcgcaaaca
     2761 accgggcatt aatcccaaaa tgttgcagca ataaaccggt gtgacgagta tcctcggcgg
     2821 caatcagatc aacggcctgt aatacctcta acgcacgctg ggtgatatcc gccagattgc
     2881 cgattggcgt cggtacaatg taaagctggc cctgagaatt atccgccgat tggtgttgtt
     2941 tcattgtgtc gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa atatcactgg
     3001 atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc tgcctgttgt
     3061 tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt ccactgctta
     3121 tatgcagggc acggcgcagg ctgattctgc cttttatctt cagcagatgc agcaaagctc
     3181 tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga aagaaggtaa
     3241 aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg attctcagcg
     3301 tcgcgagaaa acactgctgg cggtagagat taaactggcg cagaaagatt ttgctggcgc
     3361 gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc aagcccgtta
     3421 ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac tgcgcgcgtt
     3481 aattgctcag gaaccgctgc tcggcgcgaa agaaaagcag cagaatattg acgccacctg
     3541 gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca acgccgacga
     3601 aaatattctg caaggctggc tggatctgca gcgcgtctgg tttgataacc gtaacgatcc
     3661 cgacatgatg aaagccggga tcgccgactg gcaaaaacgt tatccgaaca atccgggcgc
     3721 gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct cgaccaacaa
     3781 aatcgccctg ctgttgccac tgaatggcca ggcagcggta tttggtcgca ctattcagca
     3841 aggctttgaa gcggcgaaaa atatcggcac tcagccagtg gcagctcagg tagctgccgc
     3901 acctgccgca gacgtagctg aacaacctca gccgcaaacc gtggatggcg ttgccagccc
     3961 ggcacaagcc tcggttagcg atctgaccgg tgaacagcct gcagcccagc cggtgcctgt
     4021 aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc gcaaatccat ccgcagagct
     4081 gaaaatctac gatacctcat cacaaccact tagccagatc ttaagccagg ttcagcagga
     4141 tggcgcgagt attgtggtcg gtccgttgct gaaaaataac gttgaagagt tgctgaagag
     4201 caacactccg ctgaacgtac tggcactgaa ccagccggag aatatcgaaa atcgcgtcaa
     4261 tatttgttac ttcgcgcttt caccggaaga cgaagcgcgc gatgcagcgc gtcatattcg
     4321 tgaccagggt aaacaagcgc cgctggtgct gatcccacgc agttcattgg gcgatcgcgt
     4381 agccaatgcg tttgcgcaag agtggcagaa actaggcggc ggcaccgttc tgcaacaaaa
     4441 atttggttcc accagcgaat tacgcgcggg tgttaacggc ggttctggta ttgctttaac
     4501 gggtagcccg attactccca gagcgacaac cgactccggc atgacgacca acaatccaac
     4561 gctgcaaacc acgccaaccg atgaccagtt caccaataat ggcggtcgtg tcgatgcggt
     4621 gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa ccgatgatcg ccatgcgtaa
     4681 cggtagccag agcggtgcaa cgctgtacgc cagctcccgc agtgcgcaag ggaccgctgg
     4741 cccggatttc cgactggaga tggaaggctt gcagtacagc gaaatcccga tgctggcagg
     4801 cggtaatcta ccgttaatgc agcaggcact cagcgcggtg aataacgatt attcactggc
     4861 tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca aatcatttct cacaaatgcg
     4921 ccaggttcag ggttttgaaa tcaacggtaa taccggaagc ctgacggcta acccggattg
     4981 cgtgattaac aggaagttat catggctaca gtaccaacaa ggtcaggtag tccccgccag
     5041 ttaaccacca aacagaccgg cgatgcgtgg gaagtacaag cgcgtcgctg gctggaaggc
     5101 aaaggactgc gatttgtcgc cgctaacgtg aacgagcgtg gcggcgagat cgatctgata
     5161 atgcgtgaag gccggaccac catttttgtc gaggtacgct atcgccgctc tgcgctttat
     5221 ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca aattattaca gactgcccgc
     5281 ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg attgccggtt cgatgtggta
     5341 gccttcaccg ggaatgaggt tgagtggatt aaggatgcct ttaatgacca ctcataatta
     5401 aggtttaagg attagcgtgc aagaaagaat taaagcttgc ttcactgaaa gcattcaaac
     5461 tcaaattgcg gcggcagagg cgcttccgga tgccatctcc cgtgcagcca tgacgctggt
     5521 tcagtctctg ctcaatggca acaaaatcct ctgttgtggt aatggaactt ccgctgccaa
     5581 tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa acggagcggc ccagcttacc
     5641 tgccattgca ctaaatactg ataatgttgt cttaacggcg attgccaacg atcgcttaca
     5701 tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat gcgggagatg tattgttagc
     5761 catttccacc cgtggcaaca gccgcgatat tgtaaaagcg gtagaagctg ccgttacgcg
     5821 tgatatgacc attgtggcat tgaccggcta tgacggcggc gaacttgcag gtttgttagg
     5881 gccacaagat gtggagatcc gcattccttc gcatcgtagt gctcgcattc aggaaatgca
     5941 tatgctgacg gtaaattgcc tgtgcgatct gatcgataac acgcttttcc ctcaccagga
     6001 tgattaagga gaatacatga aggcattatc gccaatcgca gtccttattt ccgcgctgct
     6061 gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct gctgtgggta ccaaagccgc
     6121 aactgaccca cgcagtgtcg gcacccaggt ggacgatggt accctggaag tgcgcgtgaa
     6181 cagcgcattg tcgaaagacg aacagattaa gaaagaagcg cgcattaatg taacggccta
     6241 tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct gaactttcgg ctcgcgccaa
     6301 acagattgct atgggcgtag acggtgccaa cgaagtgtat aacgagattc gtcagggcca
     6361 gccgattggt ctgggcgaag cgtctaacga tacgtggatc accaccaaag tgcgttcgca
     6421 gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa gtgaccaccg aaaacggtga
     6481 agtgttcctg atggggctgg tgactgaacg tgaagcgaaa gcggcggcag atattgccag
     6541 ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg tttattaaat agcagcccct
     6601 tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc tacaacgatg caaaccgtag
     6661 gtcggataag gcgtttacgc cgcatccgac atgttttccc tcaaatcaga acaacagcgc
     6721 cagcccgccg acaatcacac cagacactgc caccatcgcc cctgtcagcc ataaggcttt
     6781 cgctgggaac gctttgcgca gcataatcag tgacggcaaa ctcaccgccg ggagcgtcat
     6841 caacaatgcc agcgccggag cagttcccat acctgccagc atcatcgttt gtacaatcgg
     6901 aatttctgct gccgtgggaa tgacaaacaa gcatcctgct accgccatcg ccatcaccca
     6961 catcaggctg ttatcgacag caccatcggc atgggggaat aaccagacgc gagcggcacc
     7021 caacaccagt actgcaagga tgtaaaccgg gatcgtactc cagaaaagcg tccatagcgc
     7081 cctgccccag cggctaaaaa aaccgccctg tgcttccggt atgtcaattt cgaccggtgc
     7141 ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc gtcgcaatca gcaacaccat
     7201 caccagcccg gccaccagac gaatcgccgc aaaaccccag ccgaggacaa accccataaa
     7261 caccagcgtc gccgggttta acaccggatt gcccatccag aatgccagcg caccgcccat
     7321 cgacacctgt tggcgacgca ttcccgccgc gaccggagcc gcacagcagg tacacatcat
     7381 gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga aagcgcgatt gcccaagcgt
     7441 acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac gaaccgagga tcacccccag
     7501 cactgccgct ttccataccg cgaggaaata gatcatcgcg taatccaacg ccgcctgcca
     7561 cggattagca tccgcctgcg caaggataga tttaccgata ctgtgggttt cggcagcagt
     7621 aaacgctttg ccgtagtaag gttgccattt cacataccag agaccggcaa tgacaacgag
     7681 aaagaaaagc gcgggtttcc accactgaat gggcgttgcc gcctgagatg aagactgacc
     7741 agtcatagca ttccccagga ataagttatg ataattgagc gcgtgaatat tacgctcact
     7801 atcaattctt gggaataatt attcagctct tttgcgtaat tctgaagagc ttaaaatcgt
     7861 cacaccttca tgcccgggcc gcatcgcctc tgccagcatg acccgcgcca cgtctctcgc
     7921 atcaatggat ttccagttac cgggtaataa acggaacagc ggcgcaaaaa gcgtttcgtt
     7981 catccgctgt ttgctacgat cgcccagtaa catcgacggg cgagcaatgg tcaatttcgg
     8041 ccagttctgg gcgattaatg cttcttccat ctcccctttg acgcggttat agaaaaacgg
     8101 cgagtgggca ttggcaccca tagcactgac caccagcata tgctgcgcgc ccagtcgccg
     8161 cccggttaat gcggtatcca ctaccagcgt gtaatcggca tgaataaacg cctctttgct
     8221 ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca atgtcgatgg gatcggtgac
     8281 ctgcgccagc gcgtcagtca gttgcggatc atggggatta aacaccccgg gcatatcgcc
     8341 caacggacgt cgcgtcggcg cggctatggc gttaactttc ggttcgttaa tcaacatccg
     8401 cagcaggtga ccgcccacca gccccgttgc gcctgtaatc agtacctgac tcatcttcgc
     8461 tcctttacag aattgtctgc cttgcgctcc acggctcatg caccaggctt aatagaccat
     8521 gaggtaatta tccccgattg tgggaaattc gcctcatcca atgcaacaac gcggaggaag
     8581 catgagtaag aaaattgccg ttttaatcac tgatgaattt gaagattcag aatttacttc
     8641 acccgcagac gagttccgta aagccggaca cgaagtgatt accattgaaa aacaagcggg
     8701 taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc atcgataaat ccatcgatga
     8761 agtgacgcca gcggagtttg atgccctgct gctaccgggc ggccattcac cggattatct
     8821 gcgtggcgac aaccgttttg tcacctttac ccgtgatttt gtgaatagcg gcaaacctgt
     8881 tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc gatgtgattc gggggcgcaa
     8941 actgaccgcg gttaaaccga tcattattga tgttaaaaac gcgggcgcgg aattttacga
     9001 tcaggaagtc gtggtcgata aagatcagct ggttaccagc cggacaccgg acgatctgcc
     9061 agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga gccgcgtagc caaatcattt
     9121 ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc atcgagccgg atttcccaca
     9181 ccggggccga cagcattctg gcaaccggaa agcgacgatt gtacgccttg cgcgcgaggt
     9241 cgctttcctc accttccagc ctgcggatct cacctttaaa ctgcacgccg cgaattaacg
     9301 ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc agcctgcggc ccactcatct
     9361 gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc gactttctgc gcatcaaaca
     9421 gataaaaagc attagcgcac cagagttccc cttcctgctg cacacaccag gtgacaacat
     9481 gttgttttgc caaccaacgg ctgatggtga tgagtgtttc cattgctgtt ctcttttata
     9541 ctgtgggcct gaccttaaca tgcgacgcag acgatgacac cctggtttct ttacttgatc
     9601 cgtaccgccg acaataagct ttataccggg atcaccacgg atgtcgaacg ccgctatcag
     9661 cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact gacgctggcg
     9721 ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg cggaatatcg cgttaagcaa
     9781 ctgaccaaac ggcaaaaaga gcgtctggta gcggaaagcg cagggtttgc agagctgtta
     9841 agcagtctgc aaaccccgga gattaaaagc gattgaaatg ctcgtgatac tcaaccagac
     9901 cagttacgcc attcaacgcg tcatccgcta aacgatgtac ctggaaggcg ctttcagtgc
     9961 ctggccagcg gcaacgcagg tcgtgatgtg ccgccagttc aaagccaaaa cggctgtaca
    10021 gcgccggatc gcccagcgtc accactgcgg catagccgaa ctcattaagc gaatcgagtc
    10081 cttcatagac cagttggcgt gccagcccct gtccacggta tttttcatca accgccaacg
    10141 gtgccatgcc gacccattgc aggtcttcgc cctgcacatc aaccggacta aatgccacat
    10201 agccaatgac ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga aagccatctt
    10261 cacgcagatc gtgaaccagc ttcgcttccg catcactttc gaatgagcga cgtagcaggg
    10321 catcaatacc cggcgcatca atgggaattt ctactcgaat tagcatggtt cacctaccga
    10381 tgtctgtttg gtttcaggcg cggttttcat tcccgcctca acaaaatccg ccagttgcag
    10441 cagcatcatg cgtaaggctt tcggcatttg ctccagttca atggcgtcca tcaggttttt
    10501 cacatacagc cccagctccg tatcgccttc aatcaccagc cgacgctgga agaagagcgt
    10561 atccggatct tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg cactaaaact
    10621 cacatcagct tgcgcgttct ggctgaccac cagtttgcca ttcaccaccg aggtaaacca
    10681 ttgcaggtca atatcacgca catgaatact taaccagcgg ccttcaagaa actccagctc
    10741 gccatcatcc agcgcctgac ggaattgcca gcttaagacc tgctcaagaa cctggcgttt
    10801 tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga gatggcccca aatgcacaat
    10861 acgggaacgc agtttatcca acacgagttt tactccctgt ttcaacaatc atcctatttt
    10921 gccatatcag aaaaataaca tagcggtata aatcaacaat tccatatgaa attgctgcta
    10981 ccaccaatac aactttaact gccttaaatc aaaaattgtc gcagcaaggt taactaaaat
    11041 cccagttcgt taacattttt gcgttttgat agcgcaacct tcaggaaaaa ttatggagct
    11101 gctctgccct gccggaaatc tcccggcgct taaggcggcc atcgaaaacg gcgcagatgc
    11161 tgtttatatc gggctaaaag atgataccaa tgcccgtcac ttcgccggcc ttaactttac
    11221 cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa catcgccgca aacttcacat
    11281 cgcgattaac acttttgcgc atccggacgg ttacgcccgt tggcagcgtg ccgtggatat
    11341 ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc tagagtacgc
    11401 cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg caggcttcgg cgaccaatga
    11461 agaggcgatt aacttttatc atcgccattt tgacgttgct cgcgtggtgc tgccgcgcgt
    11521 gttgtcgatt catcaggtga aacaactggc acgggtcaca cctgtaccac tggaagtctt
    11581 tgctttcggc agcctgtgca ttatgtcgga aggtcgttgc tatctgtcgt cgtatctgac
    11641 gggtgagtcg cccaacaccg taggcgcgtg ttctccggcc cgtttcgtgc gctggcaaca
    11701 aacgccgcag gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt atcaggacgg
    11761 cgaaaacgca ggttatccga cgctatgtaa agggcgttat ctggtggacg gcgagcgcta
    11821 tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg agttaatggc
    11881 ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt agcccggcgt atgtcagcca
    11941 ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag gccgatccgc aaaacttcat
    12001 accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc gaaggcacgc agaccaccct
    12061 tggcgcgtat caccgtaaat ggcagtgaga agagcaatga aatattcctt agggccagtg
    12121 ctgtggtact ggccaaaaga gacgctggaa gatttttatc agcaggccgc caccagcagc
    12181 gccgacgtga tttatcttgg tgaagcgata tgcagcaagc gccgggcaac caaagttggc
    12241 gactggctgg agatggcaaa atcgctcgcc ggaagtggca agcagattgt gctctccacg
    12301 ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga aacgctatgt tgagaacggt
    12361 gagtttctga ttgaagccag cgatctcggc gtggtgaata tgtgcgctga acgcaaactg
    12421 ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg tgacgctgaa aatattgctc
    12481 aaacagggca tgatgcgctg gtgtatgccg gtggagcttt cccgcgactg gctggtgaat
    12541 ctgcttaatc agtgcgatga gctgggcatt cgcaaccagt ttgaagtgga agtcctgagc
    12601 tacggtcatc taccgctggc ctactccgcc cgctgcttta ccgcgcgttc ggaagaccgc
    12661 ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga acgggcgcaa cgtgctgtcg
    12721 caggaaaacc aacaagtgtt tgtactcaat ggcattcaga ccatgagcgg ctacgtttac
    12781 aacctcggta acgagctggc atccatgcag gggctggttg atgtggtccg cctgtcaccg
    12841 cagggtactg acactttcgc gatgctcgac gccttccgcg ctaatgaaaa tggcgctgcg
    12901 ccactaccgt tgacggcaaa cagcgactgt aacggctact ggcggcggct ggccgggctg
    12961 gagctgcaag cctaagtaaa tatgctcgtc atacttcaag ttgcatgtgc tgcatctgca
    13021 ttcgttcacc ccagtcactt acttatgtaa gctcctgggg attcactctc ttgtcgcctt
    13081 cctgcaactc gaattattta gagcacagct cactttgtta acaactttaa ctactcttta
    13141 atgcagtatt aaagattaat cggtaacaaa gtgagctgtt atgactgata aaaccattgc
    13201 gttttcgcta ctcgatctgg cccccattcc cgaaggttct tcagcgcgag aagcattctc
    13261 ccactctctc gatctcgccc gtctggctga aaagcgcggc tatcatcgct actggctggc
    13321 agaacaccac aatatgactg gcattgccag tgctgccacg tcggtattga ttggctatct
    13381 ggcggcgaat accaccacgc tgcatctggg gtctggcggc gtgatgttgc ctaaccactc
    13441 accgttggtc attgccgaac agttcggcac gctcaataca ctctatccgg ggcgaatcga
    13501 tttggggctg ggtcgtgctc cgggtagtga ccaacggaca atgatggcgc tacgtcgtca
    13561 tatgagcggc gatattgata atttcccccg cgatgtggcg gagctggtgg actggtttga
    13621 cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc tatggcgaga aaatccccgt
    13681 gtggttgtta ggctccagcc tttacagcgc gcaactggcg gcgcagcttg gtctgccgtt
    13741 tgcgtttgcc tcacacttcg cgccggatat gctgttccag gcgctgcatc tttatcgcag
    13801 caacttcaaa ccgtcagcac ggctggaaaa accatacgcg atggtgtgca tcaatattat
    13861 cgccgccgac agcaaccgcg acgctgaatt tctgtttacc tcaatgcagc aagcctttgt
    13921 gaagctgcgc cgtggcgaaa ccgggcaact gccgccgccg attcaaaata tggatcagtt
    13981 ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg agtatgtcgt tggtaggtga
    14041 taaagcgaaa gtgcgtcatg gcttgcagtc gatcctgcgc gaaaccgacg ccgatgagat
    14101 tatggtcaac gggcagattt tcgaccacca ggcgcggctg cattcgtttg agctggcgat
    14161 ggatgttaag gaagagttgt tgggatagtg tgtcttaacg cgggaagcct tatccgagct
    14221 ggcaacgctg tcctacgtag acctgataag cgaagcgcat caggcattgt gtaggcagca
    14281 gaaatgtcgg ataaggcacc gctgattact gatacaccgg cagtaaatta aagctcgata
    14341 aaatatggac cagtgcgttg ccaacgccaa acaccagaat cagcgtaatc atcggcttgc
    14401 cgccccagac gcggaatttc gggctgccaa agcgtttacg cgatgcgcgg gctaacagcg
    14461 ccggaacaat tgccgcccag atggtagctg ctaaaccagc ataaccaatg gcgtacagga
    14521 atccgttcgg gaacagtaga ccacccacaa caggcggggc aaaggtcagc aatgccgttt
    14581 tcaagcgccc cagggctgag tcatcgaaac caaacagatc tgccagatag tcaaacaaac
    14641 ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt tgagaacacg accagcagaa
    14701 ggtccaggct acggctgttc agcacgccgc ttaatgcctg taccagcaca tcaatattac
    14761 cgcccttctc tgcaataccg ataaactccg gacgcgggat gttacccatc gtcgccagca
    14821 accagatggt atacagcgcc agtgccatca gcgtaccgta caccaggcat ttcacgatgg
    14881 ttttcggatc tttgccgtaa tacttcatca ggcttggcac gttaccgtga taaccaaacg
    14941 atgccagaca gaacggcaag gtcatcaaca gatacggcgc ataagatgca ttgctttcag
    15001 cgacgttgaa caatgtcgca ggctgcacat gccccagcag gctaccgaag gtgaggaaga
    15061 aggtaatgac tttcgccccc agcacaatcg ctgtcatgcg actgacggct ttagtgctca
    15121 accacaccac aaacgctacc agcaatgcaa aaccaaaacc cgccgcccgt gccgggacgt
    15181 ttagtgacat ctctgcgaag gtgtgatgca gaatcgaacc actggcagaa atataggcat
    15241 aggtcaggat atagagcaca aaggcaatgg aaatgccgtt gaccacgttc cagcctttgc
    15301 ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat tcggtaattc aggttagctt
    15361 ccagaatcat caagccggaa tgcagcatac agaaccaggt aaagatcagc gccgccattg
    15421 accagaaaaa ccacgccccg gacatgacca ctggcagaga aaacatccct gcgccaataa
    15481 tggtgccgcc gataatcacc acgccgccaa gcagcgacgg tgacgtttgg gtggtggtta
    15541 gtgttgccat gagggcttct ctccagtgaa aaatagtgcg actgcgttat tatgcattgc
    15601 actgtaccag tacacgagta caaaagacag aaaaaaagcc ccgatggtaa aaatcggggc
    15661 tgtatatatt attttacaga ttgtgttcgc tgttcagcga tgattacgca tcaccaccga
    15721 aacgacgacg accggtagag tcatcacgac gcggtgcgcg gccttcacga cgttcgccgc
    15781 taaaacgacg accatcacca cgaccacctt cacggcgttc accgctgaag ttacgaccgc
    15841 cttcacgacg ctcgccaccg aaaccacgac caccgccacg acgctcaccg ccagtatgcg
    15901 gctgcgcatc gcccagcaac tgcatattca tcggcttgtt cagaatgcga gtacgcgtaa
    15961 agtgttgcag tacttcaccc ggcatacctt tcggcagttc gatagtggag tgagaagcaa
    16021 acagcttaat gttaccgatg taacggctgc tgatgtcgcc ttcgttagca atcgcgccaa
    16081 cgatatgacg aacttcaaca ccatcatcgc ggcccacttc aatgcggtaa agctgcatgt
    16141 caccaacatc acgacgttca cgacgcggac gatcttcacg gtcgccacgt gggccacggt
    16201 cgttacgatc gcgcggacca cggtcatcac ggtcacggaa ttcacgtttc ggacgcatcg
    16261 gcgcatctgg cggaacaatc agagtacgtt caccctgtgc cattttcagc agtgccgcag
    16321 ccagagtttc gagatcctgc tcttcacctt ctgcagtcgg ctgaattttg ctcagcagtg
    16381 cgcggtattg atccagatcg ctgctttcca gctgctgctg tactttagcg gcgaattttt
    16441 ccagacggcg tttgcccagc agttctgcgt tcggcagttc tacttccgga atagtcagct
    16501 tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg gttctcaacg aacagcagcg
    16561 cgcggccagc acgacccgca cgaccggtac gaccgatacg gtgaacgtaa gactcagaat
    16621 ccatcgggat atcgtagtta actaccaggc tgatacgctc aacgtccagg ccacgggctg
    16681 caacgtcggt cgcaatcagg atgtccagac gaccatcttt caggcgttcc agtgtctgct
    16741 cacgcagcgc ctggttcatg tcaccgttca gcgcggcact gttgtagccg ttacgctcaa
    16801 gagcttcagc cacttccaga gtcgcatttt tggtacgaac gaagataatc gccgcatcaa
    16861 aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt acgcataccc cagacagtcc
    16921 agtagctctg gctgatgtca ggacgggtag tcacgctgga ctgaatgcgc acttcctgcg
    16981 gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc cggcatggtt gcagagaaca
    17041 gagcggtctg atgaccttcc gggatctgcg ccataatggt ttcaacgtct tcgatgaagc
    17101 ccatgcgcag catttcgtca gcttcatcca gaaccagacc gctcagttta gagaggtcca
    17161 gagtgccacg tttcaggtgg tccagcagac ggcccggagt accgacaacg atctgcggcc
    17221 cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc gccgtacaga gcaaccacat
    17281 ttacgccgcg catgtgttta gagaaatccg tcattgcttc agcaacctgt accgccagtt
    17341 cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag ctcaggatca agattctgca
    17401 acagaggtaa agagaatgct gcagtttttc cgctccccgt ctgggccata cccagaacgt
    17461 cgcggccatt cagcagatgt ggaatacact ctgcctgaat tggagatggt ttttcgtaac
    17521 ccagatcgtt aagggcttca aggataggag ccttcaggcc cagatctgca aaagtggttt
    17581 cgaattcagc catgtagtac gtgtgcctca aaattaatgg cggccagtct acataactca
    17641 tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac cggctcaaag taggtgtatt
    17701 aacgaacaac aacgccctca cccgttaagg tgatggcaat caaaaaagat tacgggctga
    17761 tgtgtacgtc agctattgct ggtccgattc tgccaggtca tcttggtcct ggcccaggag
    17821 cgataattcc aacaatgcgt atcggtgctc aacaaagtta tgaacgttgt tggcaaccgc
    17881 cagtttgaac agtgccgtgg cgctgtccaa atcccccaga cttaggtagt acttacctaa
    17941 atagaagttg gtttcactga gatgctcagc gagcgaggtg ttatccgttg cgtccgcctt
    18001 gagcctttcc attaacgttt gttcgctaat gttgcccagg tagaactcga caatgttcca
    18061 tccccactgt tccttatccg atttttcgaa gtgctgtttc aacacttctt tagcctgctt
    18121 ctcatcgagc ttctgctcgg cgagataaag ccacagacta cggaaaggat cattgggatc
    18181 gtcttgataa aacgccagca gatcatcttg cgctaacttg tcacgaccgc cgtaatataa
    18241 tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga tcaagctcaa gtacagaatc
    18301 aaacgcttca taggcagcat caaaattgcc tgcctgcgtt aaatatatgc ctaagtaatt
    18361 gaatacttca ggcatatccg gtcggattgc cagcgcttgc gaaaaatcgt tacgcgctaa
    18421 tgccctcaga ccgagactat catacaacac tccgcgctca tataaaagct gtgcgcgttc
    18481 gtcatcggtt aaagcccgac tggcaaggat ttgttccata cgtgccagaa tcacttcctg
    18541 ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt ttacgccagg aagtattact
    18601 gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag cgcaaaaaag gcttcatttc
    18661 ccactcccga agaccacggt tgaatgaacg tcctgttccc ggttgctaac aaggcgtcct
    18721 gcccggttaa aagccccccg ccgcagcgga gggcaaatgg caaccttact cgccctgttc
    18781 agcagccgga gcttccggtg ctgcagcagg ttgagactgc tcagtcgctt ctttaatgct
    18841 cagacggata cggccctggc gatcaacttc cagaactttc accggtactt cctgacccat
    18901 ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg atttgagaga tgtggaccag
    18961 accttcttta ccgccgccga tggcaacaaa tgcgccaaag tcaacgatac gggtcacttt
    19021 accagtgtag acgcggccca cttcgatttc tgcagtgatc tcttcgatac gacgaatagc
    19081 atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca gtaccgtcat cttcgatttc
    19141 gatggtagtg ccagtttctt cggtcagggc acggataaca gagccgcctt taccgataac
    19201 gtctttgatc ttatccgggt tgatcttgat ggtatggata cgcggtgcga actcagagat
    19261 atcgccacgc ggcgcgttga tcgcctgttc cattacgccc aggatatgca gacgcgcacc
    19321 tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg ataccttcaa ttttgatatc
    19381 catctgcagt gcagagatac cgtcgcggga acctgcaact ttgaagtcca tatcgcccag
    19441 gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag ttgtcgcctt ctttcaccag
    19501 acccattgcg atacccgcaa cggcagcttt gattggcaca cctgcgtcca tcagcgccag
    19561 agacgcgccg cacacggaag ccatagaaga agaaccgttg gattcggtga tttcagacac
    19621 tacacgtacg gtgtacggga atttgtccat atccggcatg actgccagca cgccgcgctt
    19681 cgccagacga ccgtgaccaa tttcacgacg cttcggagaa ccgaccatgc cggtttcgcc
    19741 tacggagtac ggagggaagt tgtagtggaa caggaaggta tcggtacgtt cgcccatcag
    19801 ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt gcagtgacca gtgcctgcgt
    19861 ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc agcacgccag tacgcacatc
    19921 cagaccacgg atcatatctt tttcacgacc gtcgatacgc ggttcgcctg ccagtacgcg
    19981 gctacgaaca acgtttttct cgatagcgtg cagaatttca cccagttcgt tttcgtccag
    20041 ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat ttgatgacat caacctgcgc
    20101 ataacgctct tgtttgtcgg tgatgcggta agcatcgctc aggcgagctt cagccagtgc
    20161 agcaacgcgc gcgttcagcg cttcgtttac cggctccggc tgccagtccc aacgtggttt
    20221 accggcttct ttcaccagtt cattgatgtt ctgaataaca acctgctgtt gttcatgacc
    20281 gaacactact gcgcccagca tctggtcttc gctcagcagt tcagcttcag attcaaccat
    20341 cagaacagcg gcttcagtac cggcaacaac cagatccagt ttgctctctt tcagctcgtc
    20401 ctgagtcggg ttcagtacgt actggtcatt gatgtaacct acgcgagcag caccaatcgg
    20461 accattgaac ggaataccag acagagacag tgctgcggaa gcaccaatca tcgcgacgat
    20521 atccgggtta acttgcgggt taacagaaac cacggtggcg ataacctgaa cttcgttgac
    20581 gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc agacgcgcga tcagggtttc
    20641 gccttcgctt gggcggcctt cacgacggaa gaagctaccc gggatacgac cagcagcgta
    20701 ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc tgacctggtt tggctttttt
    20761 ctggccaaca acggtaacaa ataccgcggt gtcatccatg ctaaccataa cagcggcagt
    20821 agcctgacga gccatcatgc cggtttccag agtcacggtg tgttggccgt actggaattt
    20881 acgaacgatc ggattaagca atgtaatatc ctttctcttt cttagacagt accttacggc
    20941 actggtgtta atacccgatc ttctgcgcat cctcgcgact aatgacaacc ctaacccagc
    21001 tctatgtggg taaagcctct cattagccgc gcgaacctct gcaacggaag atcattcata
    21061 gcaacaatac attagtttcc agtgaattgc tgccgtcagc ttgaaaaaag gggccactca
    21121 ggcccccttt tctgaaactc gcaagaatta gcgacgcaga cccaggcgct cgatgagctg
    21181 ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc agtttacgac gctgagaaac
    21241 catgcgcagc agaccacgac ggctgtggtg atcttttttg tgctctgcaa agtggccctg
    21301 caggtggttg atctgtgcag tcagcagtgc tacctgaact tcggtagaac cggtgtcgtt
    21361 tgcgtcacga ccaaactcag aaacgatttt agctgttgct tcagtactta gagacatttt
    21421 aaaactccaa agtatataga atgaaaggac gccgatctct aattcagcga tcccagtgta
    21481 cgttacgcaa agtgttaaac aatttacgcg acgttaagcg gcagtattct actcgtagcg
    21541 acctgttatc gcaagacggt taacattacg ccgggtattc aaccaccagg cgacgaggcg
    21601 caacgcggcc ttcatcgtca atttcgccca taccgataaa tttgccgttc tcaccttctg
    21661 taacgcgaac cagtccttcc agcggcgcac cggatgtacg aactgggtta ccatttttga
    21721 agtaaacaga agacgttaac ggaagattca ccaccgggta gtccgaagct ggactgtcca
    21781 ttggcatcag taatggatca agtaactccg cagccggaat atcctgctgt tcggcttgtt
    21841 cgaccagttc acgcagatgt tccagggtca ccatccgttc aaccggatat ttacttaccg
    21901 ccagacgacg caggtaaata acatgcgcgc cacaaccgag tttttcaccc aggtcatcaa
    21961 tgatggtgcg gatataagtg ccttttgagc agtgaatttc cagctccagc tcattgcctt
    22021 cgtggcgaat aaacagcaat tcataaacgg taatcgggcg cgcttcacgc ggtacttcaa
    22081 tgccctgacg cgcatattcg tacagttttt taccctgata tttcagtgct gaatacatcg
    22141 aagggatctg ttcgatatcg ccacggaaag tatccagcgc cgccgccagt tgctctgcgc
    22201 taaaggttac cggacgttct tcaacgattt gtccgtcggc atcagaagta tcggtacgct
    22261 gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc cagcagatac tgggaaaact
    22321 tcgtcgcttc cccgaggcaa attggcaaca tgccagttgc cagcgggtcc agcgcaccgg
    22381 tatgcccggc acggttggcg ttataaatcc gtttcacttt ttgcagcgca tcattgctgg
    22441 acataccctg cggtttatcc agcaacaaaa cgccgttaat gtcgcgaccg cgacgacgag
    22501 gacgactcat tagtcctcct tgctgtcgtc cgggttaaca cgacgttctt cgtcatgttt
    22561 gaccacgctg gtcaccaggt ttgacatgcg catcccttca accagagagt tgtcgtagaa
    22621 gaaggtcagt tccggcacga tacgcaggcg catcgctttc cccagcaggc tgcggatgaa
    22681 accagaagct tcttgcaacg ctttgatgcc cgctttaacc gcgtcttcat ctttgtcgtt
    22741 gaggaacgtc acatatactt tggcatacgc caggtcgcga gacatttcga caccggaaac
    22801 ggtggtcatc atgcccaggc gaggatcttt aatttcacgc tgcaggatga gagcgatctc
    22861 tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat tctttcgcca taataaattc
    22921 tcctgacaaa aaaggggctg ttagcccctt tttaaaatta atttcaggtg gaagggctgt
    22981 tcacgttggc ctgataagac gcgccagcgt cgcatcaggc agtccatgca ggatgcggcg
    23041 taaacgcctt atcccgcatg gaaccctaaa aaccttaagc gatggtacgt tggatctcga
    23101 tgatttcgaa tacttcgatc acatcgccag tgcggacgtc gttgtagttc ttaacgccga
    23161 taccacattc cataccgtta cggacttcgt taacgtcatc tttgaagcgg cgcagggact
    23221 ccagctcgcc ttcgtagata accacgttgt cacgcagaac gcggatcggg ttgtgacgtt
    23281 taaccacacc ttcggtaacc atacagcctg cgatggcacc aaatttcggt gatttgaaca
    23341 cgtcacgaac ttccgccaga ccgataatct gctgtttcag ttccggagac agcataccgc
    23401 tcatcgccgc tttcacttcg tcaatcaggt tatagatgac ggagtagtaa cgcagatcca
    23461 ggctttccgc ttcaatcact ttacgtgcag aggcatcagc acgtacgttg aagccaacca
    23521 ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc ggtgatacca cctacgccag
    23581 aaccgatgat cttcacttta acttcgtcag tagacagttt cagcaaggag tcggagatcg
    23641 cttccacaga accctgtacg tctgccttca ggacgatgtt cacttcgtga acttcgcctt
    23701 cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg ctgacgcgcc agtttaactt
    23761 cgcggaattt accctgacga tacagtgcaa cttcacgcgc tttcttctcg tcacgtacaa
    23821 cggtaacttc atcacccgca gccggtacgc cggacaggcc gaggatttcc accggaatgg
    23881 acggacccgc ttccagcact tcctgaccca gttcgttacg catcgcacga acacgaccgt
    23941 attcgaagcc acacagaacg atatcgccct tgtgcagagt accttcacgt accagaacgg
    24001 tagcaaccgg accacgacct ttatcgagga aggattcgat aaccgcaccg ctcgccatac
    24061 ctttacgtac cgctttcagc tccagaactt ccgcctgcag caggatagcg tccagcagtt
    24121 catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa ctggctttca ccgccccact
    24181 cttccggcag gatgccatac tgggagagtt cgttcttaac acgatccgga tcggcttctg
    24241 gtttatcaat cttgttcact gcaaccacca ccggtacctg cgccgctttc gcgtgctgga
    24301 ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc aacaaccagg actacgatgt
    24361 ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc ggcgtgcccc ggggtgtcca
    24421 ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc accaatgtgc tgggtaatgc
    24481 cgcccgcttc gccagaggca actttcgttg aacgaatgta gtccagcaga gaggttttac
    24541 cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg cggttcagcc gcagcacccg
    24601 tgtcacggtc gctcattacc gcctcttcca gctcgttttc acgacgcagg ataactttgt
    24661 ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc gataacctgg ttgatggttg
    24721 ccattgcgcc cagtttcatc atcgctttga tgacctgaga gcctttaacc gccatcttgt
    24781 tcgccagttc gccaacggtg atggtttcgc cgatcacaac gtcacggtta acggcctgag
    24841 ctggcttctg gaagccttgc tgcagggaag aacctttacg ttttccgcct ttaccgccac
    24901 gtactgctgc gcgtgcttct tcacgatcag cttttgattc agcgtgtttg ttgcctttct
    24961 tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc gccttcgact tcacgatcgc
    25021 tttcgtcttc tgcctggcga gcatgttgag aagtagtgac gtgataatcg ctggaatctt
    25081 cagtcggttc cgcgttatca gtccatttgt tttcttccgc catacgacgt gcttcttcag
    25141 caacgcgacg tgcttcttct tcgagtttac gacgcgcttc ttcttcagct ttacgcttga
    25201 gctctgcagc ttcctgctca cggcgggctt tttcagcctg ggcgttttta gtcatatcgt
    25261 cttgttgatt gctcactttg tctttttccg cagcttcacg tttcgcttgt tcagcagctt
    25321 cacgcttagc ttgttctgcg gcctcacgtt cagctttttg ttgcgcctcg cgtttagccg
    25381 attcttctgc ctcacgacgg gcttgctctt ccgcttcacg ctgcgcttgc tcttccgctg
    25441 caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga
    25501 tttgtaccga tttgcttttt ccaccggtac caggaatgtt aagggtgctg cgtgttttac
    25561 gttgcagcgt caatttgtcc gggcctgaat ttttctgatt caggtggtca atcaaagtct
    25621 gtttctcttg tgcagacaca gagtcgtcag cagacttccg gatacctgca tcagcaaatt
    25681 gctgtaccag gcgttccacg gaggtctgtc gctctgcggc cagcgtttta atcgttacat
    25741 ctgtcatgct gttccttcct gctacagttt attacgcttc gtcaccgaac cagcaaatat
    25801 tacgggcagc cataatcagt gctccggctt tttcgtcggt caacccttcg atatcagcca
    25861 gatcatcaat gccctgttcg gcgagatctt ccagcgtaca aacgccacgg gcggccagtt
    25921 tgaatgccaa atcacgatct atcccttcaa ggttcagcag atcgtcagcc ggtttgttat
    25981 caccgaggct ttcttcctgg gcctgtgcaa tggtggccag tgcattttta gcacgctcgc
    26041 gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc caacagctct ttcatcggca
    26101 cataggccag ttcttccagc gtcgagaagc cttcttctac cagaacagtc gcgaagtctt
    26161 cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct
    26221 tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca accgctcagc tgcgaagcca
    26281 gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag gttaccggct tcaacggcga
    26341 tatccatggt gtgtttatct tcatccacca cgatagaagc aacgtctgcc ggtgccattg
    26401 cgttaatcac aaactgcgcc gggttatcat cccacaggac gatatcgata cgctcgccgc
    26461 ccagttcagt agaaaccgcc tgaacacgcg cgccacgcat acctacacag gcacctaccg
    26521 gatcgatacg tttatcgttg gttttcaccg cgattttcgc acgagaaccc ggatcgcgag
    26581 ccgctgcttt aatttcaatc acttcttcgc cgatttctgg cacttcaata cggaacagtt
    26641 cgatcagcat ttccggcttg gaacgagtga cgaacagttg cgcgccacgc gcttccgggc
    26701 gaacggaata gagcacgcca cgaacgcggt cgccagggcg gaagttttca cgcggcagca
    26761 tatcttcgcg caggatcacg gcttcagcat tgttgcccag atccagagag atgttgtcgc
    26821 ggtttacttt tttcaccacg ccggtgatga tttcaccttc gtgttcacgg aactgatcaa
    26881 ccaccatcgc acgttcggct tcacgcactt tctgcacgat aacctgtttt gccgtctggg
    26941 tagtgatacg gtcaaaggta acagactcaa tctgatcttc aacgtaatcg cccaggttca
    27001 ggctttcatc ttcataacgt gcggcttcaa gggtgatttc cttggtcggc tgggtgactt
    27061 catcaacaac taaccagcga cggaaggtgt caaaatcacc gcttttgcga tcgatctgta
    27121 cgcggacgtc gatctcttgt tcatattttt tctttgttgc tgtcgccagc gcgctttcca
    27181 atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt ggatacggct tcaactacag
    27241 ccaaaatttc tttgttcatc gcgggctttt cacctcatcc agactattaa aagtggggaa
    27301 ccaggttcgc cttctggata ttactcagcg cgaacacttc atctttacct tcgacggtaa
    27361 ctgtgatcat ttcaccgtct accgctttga taacgccctg ccatttacga cggttttgta
    27421 ccgccatacg gagaaccaga gtcacctctt ctccgacaaa acgggcgtag tgttcagccg
    27481 tgaacagtgg gcgatcgaga cccggtgagg agacttccag gttataagca acggtgatgg
    27541 gatcttcaac atccagcaca gcacttacct ggtggctcac atcagcacaa tcatcaacat
    27601 tgatgccatc ttcactatca atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt
    27661 cgatgccaac cagttcaaaa cccagggcct caactggcgc agtaatcatc tctgttaatt
    27721 tttgctctaa tgtggacaag cccaccccca agacataaaa aaagggccta aagcccagtt
    27781 attctgtagt cagataacaa aaaaccccga taaatcgggg ctttatataa ctgaacccta
    27841 taaccgcaac tgcggtctgg agcactttcc agaaggattt tttcaaatcc cactacgaag
    27901 gccgaagtct tcacagtata tttgaaaaag gactctaagg gaaagtggtt gcgggggccg
    27961 gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg
    28021 cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt
    28081 tggtgtaccg agacgggacg taaaatctgc aggcattata gtgatccacg ccacattttg
    28141 tcaacgttta ttgctaatca tgtgaatgaa tatccagttc actttcattt gttgaatact
    28201 tttgccttct cctgctcccc cttaagcgca ttattttaca aaaaacacac taaactcttc
    28261 ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa aattcagtga
    28321 gagcggaaat ccaggctcat catcagttaa ttaagcaggg tgttatttta tgacgacgat
    28381 tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttccggcg gtctggacac
    28441 cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat atactgcaaa
    28501 cctgggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca tggaatacgg
    28561 cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag gtattgccgc
    28621 tattcagtgt ggcgcatttc ataacaccac cggcggcctg acctatttca acacgacgcc
    28681 gctgggccgc gccgtgactg gtaccatgct ggttgctgcg atgaaagaag atggcgtgaa
    28741 tatctggggt gacggtagca cctataaagg aaacgatatc gaacgtttct atcgttatgg
    28801 tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg actttattga
    28861 tgaactgggc ggccgtcatg agatgtctga atttatgatt gcctgcggtt tcgactacaa
    28921 aatgtctgtc gaaaaagcct actccacaga ctccaacatg cttggtgcaa cgcacgaagc
    28981 gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccaatta tgggcgtgaa
    29041 attctgggat gagagcgtga agatcccggc agaagaagtc acagtacgct ttgagcaagg
    29101 tcatccggta gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga tgctggaagc
    29161 taaccgcatc ggcggtcgtc acggcctggg catgagcgac cagattgaaa accgtatcat
    29221 cgaagcgaaa agccgtggta tttacgaggc tccggggatg gcactgctac atatcgctta
    29281 tgaacgtctg ttgaccggta ttcacaacga agacaccatt gagcagtatc atgcacatgg
    29341 tcgtcagttg ggccgtctgc tgtaccaggg gcgttggttt gactcccagg cgctgatgct
    29401 gcgtgactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta ccctggagct
    29461 gcgccgtggg aacgattatt caatcctgaa taccgtctca gagaacctga cctacaagcc
    29521 agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc gtattggtca
    29581 attgaccatg cgtaatctgg atatcactga tacccgagag aaactttttg gttatgccaa
    29641 aactggcctg ctctcctcct ctgccacttc aggcgtgccg caggtggaga atctggaaaa
    29701 caaagttaag taatatcata gctttcgtgc taaaggccgc aaatgcggcc tttttttacg
    29761 gctaagagaa agtgactgcg tttaggggcc accatgtccg aattcggaat taaagtaaaa
    29821 gttaaatgaa ataaagtaaa tttcagcgca ttctattttt actttatgtt tttatgctca
    29881 gtaaaaggaa tgaccgatga atggtgaata ttttgtacgc ctcgcattac atactctgaa
    29941 atgcactcaa aaagatttag caagccactt aggtgtttcc tcaacacaaa ttagcaagtg
    30001 gaaaaaaggg gaacacatgt ccgctgatat ggaaaaaaag ttcagaaata ttacacaaat
    30061 aggagattat tctcctcaac tagttgaatg gacgggctca gtcgcgaatg ctgagaaatg
    30121 ggatcgactt atccattttc ttgctcgacg agctatggac gatgctgaaa cgggttatat
    30181 aacacaactt cttactgacg aagatggttt tttggttgaa gaaacaattg atgtattaaa
    30241 gcgaataggt tttcctacac ctctttcctt tcctgaagga cttaatattg acgacgataa
    30301 tgctgatgaa gaagaggctt tttgggagat attagagagc aatgctcatt gttctgtaat
    30361 taatgatatt tatcatgctc tgaatgatgt ttatggtttc tacatagcat atgttgatga
    30421 gttgattcag gacgacgatt tagatgtcta ttcaagcgaa gccatcaata ttcagagttc
    30481 attgatatca cttgctgcat gcaaaattga aattgatact cctgttgcgt caaactttaa
    30541 agaatttcgt tatagagtta aaaaagacta cgaaaattgg ttgaatcaac tcaagatgat
    30601 ggcattcagg gcgggaattc cgttaagagc cgaacttctg gaaatggttt ataacaccgc
    30661 tgaccaactt agcgttgcag cagaagccga acgtttcgat ttcaataaat ctcgcattca
    30721 tccagatata tacatgaacg agatccttac tggcatgaga ataattcacc aagtgcttcc
    30781 ggtaataatg caaaagttag aaattacaga tttcaaacta gatgaaacag atttatgcct
    30841 gggtaaatag ttatcaaatt aagaatactt agcctggctt ataaaacgaa aaaagacgct
    30901 tttcagcgtc tcttttctgg aatttggtac cgaggacggg acttgaaccc gtaagcccta
    30961 ttgggcacta ccacctcaag gtagcgtgtc taccaattcc accacctcgg cacggatact
    31021 actttttagt tcgggatatc gctggtcggc ttagccggag cagctggttg agtttgttcg
    31081 gttttcaccg gtgcactcag attttcccat tcgctacctt tattggtttt gttgctattg
    31141 atgttaccca gcaccagact gatgatgaag aataacgttg ccagcagcgc cgtcatgcgg
    31201 gtcatgaagt taccagaacc acttgaacca aacagcgtag cggaagcgcc tgctccgaag
    31261 gaggctccca tatcagcgcc tttaccttgc tgcagcatga tcagaccaac aaggccaatt
    31321 gccacaataa ggaaaactac taaaagagct tcgtacataa tcaacctgtt ccttgcggag
    31381 ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt ttaatctttc ccactgaagc
    31441 gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg caattttatc gcattgtatc
    31501 aactgcggaa aaaaacagca aaaccgatgt gttcgctgag aaaaaaggcg acaatcgccg
    31561 cctttttagc cagttatcta acgctttaaa cggcttttac tgcatcggcg atgcggtgtg
    31621 caaattcagt cacctgcgct tcgtcttcgc cttccaccat cacgcgaatt aacggttcgg
    31681 tgccggattt acgcagcaac acgcgtccac ggttgcccag cgcagcttca acctctgcgg
    31741 tcacggcttt aactgactca tgctcaagtg gatcgccgct acctgcggtg taacgtacgt
    31801 taaccagaat ctgcgggaac attttcatgc cgctgcaaag gtcgtgcagg ctcatatggt
    31861 tacgtgccat cgccgccagc acctgcaagc cagcaacgat gccgtcaccg gtagtagttt
    31921 tatccagcag gatcacatga ccggaattct ctgcaccgat acgccagcct ttctcctgca
    31981 ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc aaatggaatt cctaactgtt
    32041 tcagcgccag ttcaagcccc atgttgctca tcaatgtacc cacagcgcca ccacgcagct
    32101 ggccctgacg aagaccttca cgcgcgatga tatacatgat ctgatcgcca tcgactttat
    32161 tgccttcatg gtcaaccata atcacgcgat cgccatcgcc gtcgaaggca ataccgagat
    32221 ccgctttttc agccagcaca cgagcctgga gcgcgcgaac gtcggtagcc cccacttcgg
    32281 cattgatgtt tacaccgttt ggctcacaac cgatagcgat aacgttcgcc cccagttcgc
    32341 gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc acaatccacc acaatcttca
    32401 gttcactgag gctaagttcg ttcgggaacg tggctttgca aaactcgata tagcgacccg
    32461 cggcatcaac gatacggctg gctttaccca gttctgccga atcaacgcag ctgatctcct
    32521 tttccatttc cgcttcgatg gcctcttcta ccgcatccgg cagtttggtg ccgtcgatag
    32581 agaagaattt aatgccatta tcgtagaacg ggttatgcga tgcagatatc acaattccgg
    32641 cctctgcgcg gaaggtacgc gtcagataag ccacggccgg tgttggcatc gggccagtga
    32701 agagtgcgga aaggcccgct gccgccagac ccgcttccag tgctgactcc agcatatagc
    32761 cagaaatacg cgtgtcttta ccaataataa tcttacggga gccgtggcgc gccagcactt
    32821 tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt gatcggcgca tcccctacac
    32881 gaccacgaat cccatcggta ccgaaatatt tacgattact catagcgttt gttttccttt
    32941 gcagacagag tggcttccac cacccgcatc gcttctacgg tttctttgac gtcatgaaca
    33001 cgaatgatgt gcgcgccttg cattgcggca atgaccgcac aggccagact accgctcagg
    33061 cgctcggacg gccccacgtt cagcagctgc ccaatcatcg attttcgtga catacccacc
    33121 aacagcggca ggttgaaatg gtgaaattca gccaggcgcg ccagtaatga atagttatgg
    33181 gagagatttt taccgaaacc gaatccgggg tcgagcaaca atttctcttt tgcgataccc
    33241 gcctgctcgc aacgtgctat ttgctcaata aagtagcgat tcacttctgc aaagacatcg
    33301 tcatacttcg gagcttcctg catggttttt ggatttccct gcatatgcat cagacaaacc
    33361 ggtaaaccgg tttctgcagc cgcctccaga gcgccaggtt cggaaaggga gcggatatca
    33421 ttaataatgt gagcgccaac tttcgctgac tcacggatga cttctggttt ggatgtatcg
    33481 accgagatcc agacttcgaa gcgttgagca attgcctcaa ccacaggaat aacacgttgc
    33541 aactcttctt caacgctaac ttccgccgcc cctgggcgcg tggactcgcc accaacgtca
    33601 atgatcgtcg cgccagcatt gatcatcaga ttcgcatgtt tcaccgcatc tatcagcgag
    33661 ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt tgaggatccc cattacgtga
    33721 ggatggctaa ggtccagtga agtaccctgg gcaaagagtt tcatgatgtt atccctggta
    33781 tgaattgata agaaaaaacc ccggagcacg ccccggggtt ttcggtacaa atacagtcat
    33841 ctgatgcggg aacttacttg tcgcctaact gctctgacat ggtgttaccc gggttcggcg
    33901 tacgcggttc atcaaccgga cgaggagcct ttggactacc attgtcgcca gaattgttag
    33961 aagcgcctgg ttcttcccag cccgctggcg gacgtacatc gcgacgtgcc atcaggtcat
    34021 caatctgcgg tgcgtcgata gtctcatatt tcatgagagc atctttcatc gcatgcagaa
    34081 tatccatatt gtcggtcaga agctgacgcg cacgattata gttacgctca atcagtgctt
    34141 tcacttcctg gtcgatgata cgtgcagttt catcggacat atgtttcgct ttcgctacgc
    34201 tacggccgag gaatacttca ccttcttctt ccgcgtacag cagtggaccc aatttctcag
    34261 agaagcccca ctgggtcacc atgttacgtg ccagattggt cgcaacttta atatcgttgg
    34321 acgcaccggt agaaacatgt tccggcccat agatgatctc ttctgccaga cgaccaccgt
    34381 acagcgtaga aatctggctt tccagtttct gacggctggc gctgattgcg tcgccctcag
    34441 gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat aatcgtcact ttgtgcaccg
    34501 gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc cgcttcgtgg taagccgttg
    34561 attctttctg cgcttccgtc atcaccatgg agcgacgttc cgcacccatc atgattttgt
    34621 ctttcgcttt ctcgaactca accatcgaca caacgcgttt gttgccacga gcagcgaaca
    34681 gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga gaaaccagga gtaccacggg
    34741 caatgattgc cgcgtcgata tcgggggcca atggtacgcg acgcatgtga actttcagga
    34801 tctgctcacg accgcgaaca tctggcaagc cgaccacaac ctgacggtcg aaacggccag
    34861 gacgcagcag ggccgggtcg agaacgtccg gacggttagt cgcggcgata acgatgatac
    34921 cttcgttacc ttcgaagcca tccatctcaa ccagcatctg gttcagagtc tgttcacgtt
    34981 catcgtgacc accgcccaga ccagcgccac gctggcggcc tacggcgtcg atttcatcga
    35041 taaagatgat gcacggtgcc gctttcttcg cctgttcgaa catgtcacga acacgggatg
    35101 cacccacacc gacgaacatt tctacgaagt cagaaccgga gatagtaaag aacggaactt
    35161 tcgcttcgcc tgcaatcgct ttcgccagca acgttttacc ggtacccgga ggaccgacca
    35221 tcaggacgcc tttcgggatc ttaccgccga gtttctggaa gcggctcggc tcgcgcagat
    35281 actctaccag ttcagcaact tcttcttttg cttcgtcgca gcccgcaacg tcagcaaagg
    35341 tcgttttgat ctgatcttcc gtcagcatgc gcgctttgct cttaccaaac gacatggcac
    35401 ctttgccacc gccgccctgc atttgacgca tgaagaagat ccagacacca atcagcaaca
    35461 gcatcgggaa ccaggagatg aagatagaag ccagcaggct tggttcttca ggcggttcac
    35521 cgacaacctt gacgttcttg gtcaacaggt tatccagtaa tttcggatcc tgaaccggaa
    35581 tgtaagtggt ataacggtta ctatctttct tggtaacgtt gatttcacgt ccgttgatac
    35641 gcgcttcacg aacctggtcg ttattgacct cttgtaggaa ggtagagtaa tccaccttac
    35701 ggccattaga ctcgctgggc ccaaagctct ggaatactga catcagcaca acggcaatga
    35761 ccagccagag tattaggttt ttcgccatgt cactcaaggg attaacctct tattacaact
    35821 gtgttaaaaa cagcgtcagg atactctata tccagcatct ttcaaacttt cgtctgaaat
    35881 ctcccggtta gggtttacgc ccggtcgcta caatatacac ttcccgcgaa cgtgcacgag
    35941 aagagtccgg cttacgaact ttgaccttcg taaacaggga gcgaatttcc cttagatact
    36001 catcgaaacc ttcgccctgg aacaccttca ctacaaaact gccacctggc gctaatacat
    36061 cacgacacat ttctaacgcc agttccacca gatacatggc acgggggata tccaccgccg
    36121 gtgttccgct catgtttggt gccatatcgg acatgacaac ctggactttg ctgtcgccaa
    36181 cgcgctccag cagtgctttc atcaccagtt catcacgaaa atcgccctga agaaagtcca
    36241 caccaacgat aggatccata ggtaaaagat cgcaagcgat gatgcggcct ttgccgccaa
    36301 tttgggtgac cacatattgt gaccaaccac ccggagcagc accaaggtcg acaaccgtca
    36361 ttcccggttt aaagagtttg tcactttgct gtatttcatc aagtttaaac caggcacggg
    36421 aacgtaaccc ctttttctgt gcctgttgaa catatttatc gctaaagtgt tcctgaagcc
    36481 agcggctgga gctggcagaa cgcttcttac ctgtcattta actttcccat ggggataact
    36541 catcgtaacc aattgcgtaa atttttacgc gcctatttgg tgatatatgg gagatggcgg
    36601 tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata cgatgaatct gagtactaaa
    36661 caaaaacagc acctgaaagg tctggcacat ccgctcaagc cagttgttct gcttggcagt
    36721 aatggtttga ccgaaggggt gctggccgag attgaacaag cgttagagca ccatgaactc
    36781 atcaaggtga aaatcgccac cgaagatcgc gaaactaaaa ccttgatcgt ggaagctatc
    36841 gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta aaacgctggt gctttatcgc
    36901 ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt atcctaaagt tacacacatt
    36961 tgctgtgtaa aacgaggggt tttccgcagg caggagagca aaatgccacg ctctgttcgt
    37021 tgataaaagg ccgcatagcg gcctttttcc tttctttaca atacatcaac atcttgagta
    37081 ttgggtaatt cttacaggta ttccacctta attacttcaa attctacttc gccgcccggc
    37141 gttttgatga ccacaacatc atcttcttct ttgccgatca ggccacgagc aataggcgag
    37201 tttacagaaa tcaggttttg tttaaagtca gcttcgtcat cgccaacgat gcgataagtc
    37261 tgttcttcgt cagaatccag attcagcacc gttacggtag caccaaaaat aacgcgccca
    37321 ttgttgggca ttttggtgac atcaatcacc tgcgcgttcg acagcttggc ttcgatgtct
    37381 ttaatacggc cttcgcagaa accctgctgt tcacgagctg cgtggtattc ggcgttttct
    37441 ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa tgatttcagg acggcgcaca
    37501 gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc cgcgtaaggt catcggaata
    37561 gcttgcattt gttatacctc ttgaatattc ctgatggggc aagtcttcac ccaccccggc
    37621 tgttcggcct gcccggcata actcctcacc gggaccagaa gcaaaaaaat accgacccgg
    37681 gtacaagtcc caggtcagct acaattcaca ttttgatagt cattttaccc tgaagttccc
    37741 gaagggtcat cgtttacttt atagggcgtt gcgccgtagt atgacggctc gattccaggt
    37801 tgttagcgcg agattatgcg attttccaga tttatcatcg gattgaccag ctgtatagcg
    37861 ttcagtgttc aggccgcgaa tgttgatgag tacgttactc aactccccgc tggtgccaac
    37921 cttgccctga tggtgcaaaa agtcggcgcg tcggcccccg ctattgatta ccacagtcag
    37981 cagatggcgc tgcccgccag tacgcagaaa gtgattactg cgctggcggc gttgattcaa
    38041 ctcggccccg attttcgttt caccacgacg cttgaaacca aaggcaatgt ggaaaacggc
    38101 gtacttaagg gtgacttagt ggcgcgattt ggtgccgatc cgacgttaaa acgtcaggat
    38161 attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca accaaatcga tggcaatgtg
    38221 ttgatagata cctccatttt cgccagccac gataaagccc ccggctggcc atggaatgac
    38281 atgacacaat gctttagcgc tccgcctgcc gctgccatag ttgaccgcaa ctgtttctcc
    38341 gtctcgctct acagtgcccc aaaacctggt gatatggctt ttatacgcgt ggcatcttat
    38401 taccccgtta cgatgttcag ccaggtacgc accctccccc gaggttctgc cgaagcgcaa
    38461 tactgcgaac tggatgtcgt accaggcgac ctgaaccgct ttacgctgac gggatgcctg
    38521 ccacaacgtt ctgagccgct cccgttggct tttgccgtgc aggatggagc cagctatgcc
    38581 ggggcaattc tgaaagatga gttaaaacag gcaggtatca cctggagcgg aacactgctg
    38641 cgccagactc aggttaacga acctggaacg gtagttgcca gtaaacagtc ggccccgttg
    38701 cacgatctgc ttaagattat gctgaaaaag tcggacaaca tgatcgccga tacggttttc
    38761 cgcatgatag gccatgcgcg cttcaatgtg cctggaacat ggcgggccgg gtcggacgcc
    38821 gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg gaaacaccat tattgccgat
    38881 ggttcagggc tttcgcggca taacctgatt gcccccgcca ccatgatgca ggtgttgcaa
    38941 tacattgccc aacacgacaa tgaacttaac tttatctcca tgctgccgct ggcgggctat
    39001 gacggctctt tgcagtaccg tgcaggtctg catcaggcgg gcgtggatgg aaaagtctca
    39061 gcgaaaaccg gttcgttgca gggggtatat aacctggcgg gattcattac cacagcgagc
    39121 gggcaacgaa tggcgtttgt acaatatctt tctggctatg cagtagaacc ggcggatcag
    39181 cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt tgtataaaga tatttatcag
    39241 aacaattagt caaaaagaaa ccccggcaca tggccggggt tttgctgatt aacgcttgta
    39301 aatgaactca acgccttctt cgtcgtcttc gtcccagtca tcatcccagt cctcatcatc
    39361 ctcttcagca atctcttcaa gctgctggcg atgataatca tcccacatga attcgacttt
    39421 ctctggctgt ttcgcttctt cagcctgcac gaccgggttt tcaatgataa aggtcatcac
    39481 atcccagcag agatctttca cgcccagtcc actcgccgca gagatcagat aatatttatc
    39541 ttcccagccc agcgcttcag caatcgcttt cgctttctct tcagcttctg ccttatccag
    39601 cagatcgatc ttattgaaca ctaaccaacg cggtttcgcc gccagatcct ggctgtattt
    39661 ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga tcggtgccgt caatcggatc
    39721 gatatcgatg aggtgcaaca ggacgcggca acgttccagg tgcttcagga agcggatacc
    39781 cagacctgcg ccttccgcag cgccttcaat cagtcctgga atatcggcaa caacgaagct
    39841 cttttcgttg tccattcgta ccacacccag acttggcacc agagtggtaa acggataatc
    39901 cgccactttc ggtttagccg ccgataccgc acgaataaag gtcgatttac ccgcgtttgg
    39961 catccccaac ataccgacgt cagccagcag catcagctcc agcagcagct cacgcttatc
    40021 gcccggcgta ccgttagttt tctgccgtgg tgtacggtta actgacgatt tgaaacgggt
    40081 attgcccaga ccgtgccagc cgcccttagc aaccagcaga cgctgaccgt gtttggtcat
    40141 atcgcccatg gtttcaccag taccctggtc gattacacgc gtacctaccg gcactttaat
    40201 tgtgacgtct ttaccgcgct taccggtaca atcgcggctt gcgccgttct gaccgcgctc
    40261 tgcacggaaa gatttttcaa aacgataatc gataagcgtg ttcaggtttt cgtcggcttc
    40321 catccatacg tcaccaccat caccaccgtc gccgccatcc gggccgcctt tcggaatata
    40381 cttttcgcgg cggaagctca cgcaaccatt accgccatca cctgcaacga ccagaatcga
    40441 tgcttcatca acaaacttca ttttattctc cgtaaatcat tcgcctgcgc ggggttgcga
    40501 taccaccgtt gtatgcttac gtaatccgcc ccaaatacga tgaccaatgg cggaatacat
    40561 cgcgcccgca accacgacaa acgcaccgag ataacctaaa aggtttaaca tcggtctggc
    40621 gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa aacagcgtaa acagtggggt
    40681 gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga gccatcgctt ccgccagggc
    40741 gccatatcct accaatgtat tcagtccgca aaaaattaaa catgcgagct gccagtggct
    40801 aagctgcgct atcactccag gctttgccag agggaagagc gcaattgtac ataaagtgta
    40861 cagtaaaaac aggatctgcg gtgaggccag ccgacgcaat aaaacctttt gcgccacgcc
    40921 ataactcacc caaaccgtcg ccgcaccgac cccaaagata actccccagg tgtaatcggt
    40981 gagctttgta aatatctcga ccagactggt gttaaaaaac atcaccaggc cgctcaggag
    41041 catcaatgcc cctacaacct gagtgctgcg cattttctct ttcaggataa atacgctggc
    41101 aaccatcatg ccaactggcg agagttgccc aatcacctgc gaagcggtcg gactcaggta
    41161 ttgcaaggat gagctgaaca ggatgaagtt cccaaacagc ccggcggtcg ccactgccaa
    41221 caaaatcaac cagcgtggct tacgaaacac gcgtaatggc ggcaacctct tcttcaccgc
    41281 aagaatggca ccaaggccaa tactcgccat caagaaacgg taaaacacga ttgtcggagg
    41341 ttccatcacc tccagcacct gcttcattgc gattggcaac gccccccagc aaattgctgt
    41401 ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc ataccgtatt ccctgcaaag
    41461 cgcatttgcg gattaacgtc gcaccatgcg catttttacc ggttatcgaa tgtaaaaagc
    41521 cccgcaacgt gttgcggggc tttcatccgt taccgggacg cgaaaaactt attcagcttc
    41581 gatgctgata aatttacggt ttttcgggcc tttaacttcg aatttcactt taccgtctgc
    41641 tttagcaaac agagtgtggt cacgaccgca acctacgtta gcgccagcgt ggaatttggt
    41701 accacgttga cgaacgatga tgctacccgc cagaacggat tcgccaccga aacgcttaac
    41761 gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg gagccgccag cctttttatg
    41821 tgccatttga aatctctcct caggtcttag gcgctgatgc cagtaatttt cacatcagtg
    41881 aaccactgac gatggccctg ctgcttacga tagtgtttac gacgacgaaa cttaacgatt
    41941 ttaactttct cgccacgacc gtgagcaaca acttcagctt tgattacgcc gccatcaacg
    42001 aaaggaacgc cgattttgac ttcttcaccg tttgcgatca tcagcacttc agcgaactca
    42061 acagtttcgc cagttgcgat gtccagcttt tccaggcgaa cggtctgacc ttcgcttact
    42121 cggtgttgtt taccaccact ttggaaaacc gcgtacatat aaaactccgc ttccgcgcac
    42181 accttttcaa tgattcagag tgcgctataa atattcacaa tagggcgcga atattacgca
    42241 aaacgcacgc ctttgacaag tgctacagtc aatacacgaa gaaaaaaaac acaacttgta
    42301 cggtaacgtt tatctgtgcc attttttcag tacaatcact ctatattcct aaccataaac
    42361 cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa aagcccggct tttgcgatga
    42421 atttagaaaa aatcaatgag ttaaccgcgc aagatatggc gggtgttaat gcggcaatcc
    42481 ttgagcagct taattccgac gtccagctga tcaatcagtt aggctattac atcgtcagcg
    42541 gcggcggtaa acgtattcgt ccgatgattg ctgtactggc tgcacgagct gttggctatg
    42601 agggaaatgc gcatgtcacc attgctgccc tgatcgagtt tatccacacg gcgactctgc
    42661 tgcacgacga cgttgtggat gaatcagata tgcgcagggg taaagctacc gccaacgctg
    42721 catttggcaa tgccgccagc gtgctggtag gcgattttat ttatacccgc gcgttccaga
    42781 tgatgaccag cctcggttcg ctcaaagtgc tggaagtcat gtcagaagcc gtaaacgtca
    42841 tcgcagaagg tgaagttctg caactgatga acgttaacga tccggacatc actgaagaaa
    42901 actacatgcg cgttatctat agcaaaaccg cgcgtctgtt tgaggctgcc gcgcagtgtt
    42961 ccgggattct ggctggctgt acgccggaag aggagaaagg cctgcaggat tatgggcgct
    43021 atctcggcac tgctttccag ttgatcgacg atttactcga ttacaatgcc gatggcgaac
    43081 agttaggtaa aaatgtcggc gacgatctga acgaaggtaa accgacgctg ccgctgctgc
    43141 atgcgatgca tcatggcaca ccagaacagg cacagatgat ccgtaccgcc atcgaacagg
    43201 gtaacggtcg ccatcttctg gaaccggttc tggaagcaat gaacgcttgt ggatctcttg
    43261 aatggacgcg tcagcgtgcc gaggaagaag cagacaaagc catcgcagca ttacaggtgc
    43321 tcccggacac cccttggcga gaagcactca tcggcctcgc gcacatcgct gttcaacgcg
    43381 atcgttaatc ccctcccttc atcccgcgca gtgcgcggga tgattccagt aaattccata
    43441 aaaacactaa ttcagcgcta taacacctga aaaaaagggc tgtgcatctt tatatattct
    43501 gaatattcac actttttaca ggaacttttt agagcaatag gccatcagga gtatagtgat
    43561 gcccgacaga agaagtgttc tgaatgaaag cgactaactt aaggagtgag gaaagatgga
    43621 aagtaatttc attgactggc atcccgctga catcattgcg ggtttgcgca agaagggaac
    43681 gtcaatggcg gcggagtctc gcagaaatgg tttgagttcc tcaacgctgg caaacgcatt
    43741 atcgcgccca tggccgaaag gagagatgat tattgcgaaa gccctgggaa ctgacccctg
    43801 ggttatctgg ccatcacgct accatgatcc gcagacccat gagtttatcg acagaacgca
    43861 gttgatgcgt agctacacta aaccgaaaaa atgagtggtc tggcggtagc cccgcgaacg
    43921 gggctgccag ctctcagacg attactcgcc tttcacacgc tcaatatttg cacctaaagc
    43981 gcgcagtttg tcttcaatgc gttcgtagcc acgatcgatg tgataaatac gatcaaccac
    44041 cgtcgtccct tccgcaatac agccagccag caccaggctt gctgatgcac gcagatcggt
    44101 tgccataacc tgtgcgccag aaagtttttc aacaccgtga caaataacgg tattgctttc
    44161 gatttcggca tgcgcgccca tacggctaag ctccggcaca tgcataaagc ggttttcaaa
    44221 gaccgtttcg gtgatgaatc cggtcccttc tgccaccagg ttcaacagcg tgaactgggc
    44281 ctgcatatcg gtcgggaatg ccggatgcgg cgcggtacgt acgttaacag ccttcggacg
    44341 tttgccatgc atatccaggc taatccagtc ttcgccgact tcgatgtccg ctccagcgtc
    44401 acgcagtttc gccagcacgg cgtcgagagt atctggctgc gcgttacggc agataatttt
    44461 gccgcgagaa atcgccgccg ccaccaggaa agtaccggtt tcgatacgat ccggtagcac
    44521 gcgatagaca ccgccgccta aacgttccac accttcgatg acgatacgat cggtgccctg
    44581 accgctaatt ttcgcaccca gcgtaatcag gaagttcgcg gtatcgacga tttccggttc
    44641 acgcgctgcg ttttcaataa tcgtggtacc ttccgccagg gttgcagcac acatgatggt
    44701 caccgttgcg ccaacgctga ctttatccat cacgatatgt gcgcctttca gacgaccatc
    44761 gacggaagct ttaacgtaac cttcttccag tttgatggtc gcgcctaatt gttcgaggcc
    44821 agaaatgtgt agatcaaccg gacgcgcacc gatcgtacaa ccgccaggca gtgaaacttg
    44881 cccctgacca aagcgcgcta ccagcggccc cagcgcccag atagaagcac gcatggtttt
    44941 aaccagatcg taaggtgcgc agaatacatt aacgtcgcgg gcatcaatat gcacagaacc
    45001 attacgttct actttcgcac ccagctggct tagcagcttc attgatgtat cgacgtcttt
    45061 cagtttcggg acgttctgga tctctaccgg ttcttccgcc agtagtgcgg caaaaaggat
    45121 aggcagagca gcatttttag cgccggaaat tgtgacttcg ccctggagct tcgttggccc
    45181 ctgaacacga aatttatcca tttagtttgt tctcagttaa caattcatat ccgctaccgg
    45241 cgaatcgccc atagctcaaa agccgttcag tttgcgatcg cgcgcccact ccgcaggggt
    45301 atacgctttg atcgacacag catgaatgcg gttatccgca atatattcca tcagcgggcc
    45361 atagaccgtc tgctgttttt taacccgact catgccgtca aacaactcac ccacggcaat
    45421 aacctgaaag tggctgccat cgccggaaac gtggacttcc tggagggaga gagcgttcat
    45481 caacacgctc tgaatttcat tattttccat gggatcttca atcatcagtt aataaaccag
    45541 cgaaacatct tagagcaaag ttgcgctggc ataaataagc aaaaagcctc gctgataaat
    45601 cagacaaggc tcgacttgca ggcaggtttg ccggacaggc ggttaacgcc atatccggcc
    45661 tgaaaaaatt taacgaggca gaacatcagc aggcaaatta tacaatttcg ccagggtata
    45721 cactttgtcg tttaccccct gaagcgtcac attgttgccc tgctttttcg ccagatcgat
    45781 aagatggagc agcagtgcca gtccacccgt atccacgcgg gacacacggc taagatcgat
    45841 gcaggtaacc cccttcaccg cttcctcacg catttcccaa agcggtagca gaacgtcctg
    45901 atccagctct ccggataacg ccagcgtgtc acccgtctgc atccagctca gtgactcgct
    45961 cattattttt tctcttccag agtgattttc tgttgagaaa tcgatttcag ttgcgcagtc
    46021 aggccgtcga tacctttggt acgcagcagc gttccccact cgttttgttt ggtggtgatc
    46081 atactgacgc cttcagcaat catgtcgtaa gcctgccagt ttcccgtctg ggagttttta
    46141 cgccactgga agtccagacg caccggcggg cggccattcg ggtcaataat ggtaacgcga
    46201 ataggcacaa tggttttatc gcccagcggc tgttctggcg caatctgata ggtttgaccg
    46261 tgatacatcg ccagcgcctg accgtaagcc tgcttcaggt actcacggaa agcggcaaag
    46321 taggcttcac gttgagcagg ggtcgcactc ttgtaatact ggcccagcac cagcgcaccg
    46381 gcgtatttca cctgtacgta tggcagcagt tcctgatcaa caatggtacg cagataatcc
    46441 gggttggccc gaatttgcgg ttgctcattc ttcaggcgat cgaacgtttt ctgcgccgcc
    46501 tcgtccatca gcttatacgg attggtctgg tctgccgcgg ttgccgcact cagaggtgca
    46561 atcaccagca aagcgaccat cattaaacgt ttaaacatgc gtcggttctc ctgaaattat
    46621 ttcgttgtac ccacaggttc agtggtttca ttattacctg gcgcagcagc tggcgcatcg
    46681 ccactattct tattgtcatc gcctttacta ccgtaaagga actgaccaat gagatcttcc
    46741 agcaccatcg cagacttagt gtcctgaatt gtatcgccat ccttcaggat agcagtcccc
    46801 agttccgggt cttcaaaacc gacgtttaat gccagatatt gttcccccag caggccggaa
    46861 gtacgaatgc tcagcgaact ggtatctgga atgtggttat aacgttgttc aatttccagc
    46921 gttacgcgcg gcagataggt tttcgggtcc agcgtaatat ccgccacccg acccacaaca
    46981 acgccaccaa tactgaccgg agagcgcgct ttcaggccgc caatgttatc gaacgtcgca
    47041 taaagcgtgt aggtcggttc agtacgtatg gacgtcacgt tcgccgcctt caggcaaaca
    47101 aacagcgccg ccagcagtgc tgctaataaa aagataccca cccaaatttc attttttttc
    47161 gtttgcatga actcaattcc caaacatcaa tgcggtcagc acaaaatcca gccccagaac
    47221 agccagagac gagtggacaa cggtgcgagt ggttgcccgg ctaatcccgg cagacgtcgg
    47281 gatggcgtcg tagccgttaa acaacgaaat ccacgtcacc gtgatggcga acaccacgct
    47341 cttaatcaga cagttgacca gatccatacg ccagtcgacg gcattttgca ttgccgacca
    47401 gaagaacccg ctatcaatgc ctttccaact gacgccgacc agagatccgc cccagatccc
    47461 cacggcgacg aaaataaccg tcaacagtgg taatgaaata accccagccc agaaacgggg
    47521 agaaataacc cgacgcagcg gatccaccgc catcatctcc atactggaga gttgctctgt
    47581 agcgcgcatc aggccgattt ctgcggttag cgccgaacca gcacgcccgg caaacaacaa
    47641 cgcggcaacc accggcccca gttcacgcag tagcgataac gccaccagca tacccagact
    47701 ggtttccgca ctataagtgg tcagaaccag ataaccttgc agccccaaca ccattccgat
    47761 gaacacgcca gaaaccacaa taatcagcat cgacaggacg ccgacattat agagctggcg
    47821 caccagcagc ggcgcatgtt tgcgaaattc cggtttgccg accagcgcat tgaataacat
    47881 taacccggcc cgcccgaacg ttctcagggt tttaatccct ttatgtccga gcgacgccag
    47941 cgcatttaac agcatgagtg gcttaactcc ctggtaaaag atcagcgtga taatcgccgg
    48001 caggatagcg gaacggaaca ggcccgtcag ctatcccgtc cagaaactga cgtacgcgcg
    48061 gatcaggatt cgcctgcaac gcctgggcac tgccatgagc gacaattttt ttgtccgcca
    48121 ggatccaggc gtgatccgca atacttaaca cttccggcac atcgtgagaa accaccacac
    48181 aagtcacgcc cagcgcgctg ttcagctcag aaatcagctt caccagtacg cccatggtga
    48241 tgggatcttg cccaacaaaa ggttcatcaa acatgatgag atccggctcc agcgcaatcg
    48301 cacgcgccag cgctgcacgc cgcgccatcc caccagaaag ttcagaaggc attagcttag
    48361 ccgctccacg cagccccacg gcctccagct tcatcatcac tgtactatgc aacaatggcg
    48421 cgggaagttg ggtatgttcg cgcagtggat aggcgacgtt gtcaaatacg ttcatatcag
    48481 tgaacaacgc cccggactga aataacatgc tcatccgttt gcgcactgta tacaggcgcg
    48541 aacgagacat cgccggaata ttctcaccat cgaaaaggat ctcaccatga tctggtgcga
    48601 tttgcccgcc aatcagacgg agtagcgtcg ttttaccgat gcccgatggc cccatgattg
    48661 ccgtgatctt ccctcgcggc acggtcaggg aaatattatc gaagatgcag cgattgccac
    48721 gcgtaaaact gacatcgcgc atatcgacta aattcgccac agactgctcc ataattcacc
    48781 cttcgtcttg cgttgatttt ctaagcatgg cgctcaattt aaccttgaac ccaacatatt
    48841 tacagaatat tacccgccgt ggttagcgaa agctggcatt tgttttactt tttagccgca
    48901 taaagtcaaa attaagaatc cgttacggct ttctgaaaat cttcagcgga ccggcgagta
    48961 tacctgaaga aaggatgtta gatgctttta gctacggcac tgttaattgt tggtttactt
    49021 ttggtcgttt acagtgccga ccgcctggtt tttgccgcgt ctattctttg ccgaaccttt
    49081 ggcatcccgc cgctgatcat cggcatgacg gtggtcagta ttggtacatc gttaccagaa
    49141 gtcatcgtct cgcttgccgc gtctctgcac gaacaacgcg atttagccgt tggtacagcc
    49201 ctcggctcaa acattatcaa tatattgctg atcctcggtc tggctgcgct ggttcgtcct
    49261 tttaccgtcc attctgatgt tctacgccgt gaattaccct taatgttgtt ggtcagcgtg
    49321 gtggccggtt ccgtactcta tgacggacaa cttagtcgaa gcgatggtat ctttctcctc
    49381 tttctggctg tgctatggct gctgttcatt gttaaacttg cacgtcaggc tgaacgtcag
    49441 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    49501 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    49561 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatgggtctg
    49621 acggcaattg ctatcggaac cagcctgccg gaactggcaa ccgcaatagc gggggttcgc
    49681 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    49741 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccatt ggcgtacagt
    49801 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    49861 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    49921 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    49981 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50041 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50101 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50161 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50221 tggtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    50281 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    50341 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    50401 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    50461 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    50521 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    50581 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    50641 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    50701 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    50761 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    50821 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    50881 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    50941 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51001 cttgcgacct gttacggccc tgtcagcgcc gacgttatag caaaagcaga gaacattcgt
    51061 ctgctgatcc tcgatgtcga tggcgtactg tcagatggcc tgatttatat gggcaataat
    51121 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51181 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51241 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    51301 gatttgctgg aaaaactggc gattgccccg gaaaatgtgg cttatgtcgg cgatgatctc
    51361 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    51421 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    51481 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    51541 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    51601 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    51661 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    51721 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    51781 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    51841 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    51901 tcgtgccgga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    51961 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52021 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52081 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52141 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52201 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52261 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggttgttac ccgtccgggc
    52321 ggcgaacaag gtaaagaagt gattgacggc tacggtaaac cggcaacgtt ctaccagatg
    52381 caggacaacg gtaaaccggt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    52441 gattttgtcg ttctgacggg taatgcttat ctgcagcagg ttgatagcaa cattaagggc
    52501 gataagatca cttacctggt aaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    52561 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    52621 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    52681 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    52741 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    52801 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    52861 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    52921 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    52981 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53041 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53101 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53161 atctcggtta tcgacattaa acgcatcatt gagcacctgc gcgacagcgg cctgggcgtg
    53221 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    53281 agtcaggggc atttgatcgc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    53341 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    53401 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    53461 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    53521 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    53581 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    53641 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    53701 ccgctggtac accatccggc accagcggtg actacattga cgatgagctg ccgatctatc
    53761 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    53821 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gacaccggtt
    53881 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    53941 acgaggttga agccgtcctt aagcggatcc aacggtttga tccggtcggt gtagcggcaa
    54001 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataaaacc acgccgtggc
    54061 tggaagaggc cagactgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54121 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54181 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54241 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    54301 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    54361 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    54421 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    54481 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    54541 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    54601 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    54661 gcgaagcttc ctccacggcg attcgtgcgc tggtgaagaa attaatcgcg gcggaaaacc
    54721 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    54781 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    54841 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    54901 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagccaaatt tgccaaactt
    54961 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55021 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55081 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55141 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55201 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55261 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    55321 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    55381 gaaatcatca gcgagctggc ggcgaaacaa cttagtctgc cgcctcaggt ggtttttgaa
    55441 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    55501 catggcaaac tggaagaaga tactctgcgc gccgttggcg ttttcgttca gctcgaaacg
    55561 cctatagctt tcgatgccat cgacaaccaa ccggtggatt tactttttgc cctgctggtg
    55621 ccggcagacc aaactaaaac gcacctgcat actctgtcac tggtggcgaa acgtctggcg
    55681 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    55741 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    55801 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    55861 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    55921 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    55981 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56041 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56101 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56161 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56221 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    56281 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    56341 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    56401 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    56461 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    56521 gaaaccaaca accgtagcta cttgacggtc gccattggtt gtaccggcgg gaagcaccgt
    56581 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    56641 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    56701 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    56761 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggccgaagcc aacagcgtta
    56821 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggcc
    56881 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taactctggt tttgatgaag
    56941 attaatcttc atcactttga catacaactc ccttcaaact ccccccgaca ataagaaaat
    57001 cacgtactga aatcattctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57061 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57121 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57181 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57241 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    57301 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    57361 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgca gattttgacg
    57421 gcaaaccctc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    57481 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    57541 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    57601 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    57661 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    57721 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    57781 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    57841 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    57901 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    57961 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58021 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58081 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58141 ttttggccgt ctgctgagaa atcgttgaag caccgcgaat acggttttca ttgcgctcgt
    58201 tgtgcgccag ggctttctca atggaagcga catcaaagcc ccagtgctca ggaaatttct
    58261 gatcttctgc ggcaatcacc gccagtccca tccacggcga gatttgatcc atactgaccc
    58321 agtcagaatg tgccacgtaa cgaaaattgc catgcagcca ggcgctgacc tgtcgctcga
    58381 ccattaccgc tgagaaggga acaggcgcaa cactaaacaa cgcgatgccc ccgccccaga
    58441 aaacggcgag gacaaccatt aaccgcaaaa gaaaacggcg aacgaaacta aacaccgtta
    58501 agcggctttt actcattcag ccagaaccag cacgcgggaa accagcttat caatgccgct
    58561 cgccgcttct gcaatgttct gcgccagcat atatgctggg gtggtgacaa tcttattgtc
    58621 ttcatcaacc acgatatcat cgacaggaca gggcacatgc tccgcgccca tctcttccag
    58681 cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat cgaaaatttt
    58741 cggcagcatc gccggggcaa tacacataaa accaagcggt tttccggcct gatgcatcgc
    58801 ttgtgccagc gcctttaatt cacggtcaac ggtgcattcg ctaccaagag tggcaaaatt
    58861 gcttaaattc ttcgccgcgc caaacccccc cggcacaatc aacgcatcca gttcagcggc
    58921 atcggcctgg gccagaggac ggatttcacc gcgcgttatt cgtgccgctt caatcagcac
    58981 gttgcgcgtt tccgtcatcg cttcgccagt taaatggttg ataacatcaa cctgctgctt
    59041 atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca acgtcaacac
    59101 cgcttcatga atttcagaac cgtcatagac gccgcatccg ctcagaatta cgccaatttt
    59161 cttcattgtg atcatccttt cgcaattgac tgaaacacat taattttttt aataaaaatg
    59221 gtacgcatca cacatttaac tgattcatgt aacaaatcat ttaagttttg ctatcttaac
    59281 tgcgtgcggc ctgaaaaaca gtgctgtgcc cttgtaactc atcataataa tttacggcgc
    59341 agccaagatt tccctggtgt tggcgcagta ttcgcgcacc ccggtctagc cggggtcatt
    59401 ttttagtggc ttttgccacc cacgctttca gcacttctac gtcgtgacgc cactcttctt
    59461 tcatctcttc aatccattca ccgacgttat cttcccaggc aggaaggtcc ggagactgaa
    59521 tttgctgacc cagctgttgc agatggcgta accccactga ccccgccgca cctttaattt
    59581 tatgtccttc ctctacaatg ccttttttat cctgcgccgt cagattcgac tccagcacgc
    59641 tgacatagcc cggcatcatc ttctcaaaca ccgctaaccc gtcggtgatc agcttcggtc
    59701 ctacaagttc gagatactgt tccagcatgg gaatatcgag caatgcttct gatttactgt
    59761 tctcttctgt cgtcaccgta ctctcctcat catcctgggt atcccagaat ttcttgatca
    59821 tcgcggttag cgccggaacc gaaagcggct tactcagcac atcatccatt ccagcattga
    59881 ggtactcttg tttgtctttc agcacgttag cggttaaggc caccagcggc ggtaaatcct
    59941 cgcgcggata acgcttcgtc agttcacgag agatatccag cccggtcata tctggcaact
    60001 gaatatccag caacaccagg tcgtattcgc ccggtttaaa catctccagc gccgccttgc
    60061 cggtcatggc gacatcaacg ctgttaccta atttttccag cacagaacgc gcaacaatca
    60121 cgttcagttc aatgtcttcc accagcagca cattcagcgc cggtaaaggc atatcgtctt
    60181 catcaaacgc atcatcgacc tcttctgcta ccgacggtgc gtggatcgtc aacgtaaagg
    60241 ttgaaccttt gccctgttcg ctggtaaccg taatatcgcc gcccatattt ttcgccagac
    60301 gacgagaaac ggccagacca ataccggtgc cggtggcagg tttaccgcca tgactgtctt
    60361 tcacctggta atacatggcg aaaattttat ccagctcatc ctgcggaatg ccgataccag
    60421 agtcttccac ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc acggtaacct
    60481 ggccttgctg ggtgaatttg acggcgttac tgatgaggtt ccacaggatc tgccgtaaac
    60541 gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt cggctccagg ttaaagcgca
    60601 atcctttttg ttgcgcctgc aatgcggaga gattttccag atcggcaagg aagctggtga
    60661 aatcaaccgg ttgattatca agctggacct tgcgccgttc catcttatcc atgtcgataa
    60721 tatcgttaaa gatattcccc agcgtgacgg ccgaaacatg gatggtcttg agatattttt
    60781 cctgctcggc ggtgagttcg gtatccagca gaatacggct caggccgacg ataccgttca
    60841 gcggtgtacg caattcgtga ctgatggtgg agataaacgt cgttttgtcg cggctggccc
    60901 gttcaagcgc atcctgatac cgcttacgct cggtaatgtc gcgaccaaag cccatcaaac
    60961 cgtgacgttt acccacgcgg tcgtagtacg gcactttacg gatttcaaag caggctttgc
    61021 gcccgtccgg gtaatccagc cactgttcat aggtcagtga cacattatga cggaacactt
    61081 tttcatcggt ttcaatgact tttgcggcgg cttccggtga gtaaacatca gcaggtttca
    61141 ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg ttacagccgg
    61201 aaaactcttt atcttcgtta cgataaaaaa ccaggtcggg tgaagcatca aggaaggaac
    61261 gtaagaatga ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa
    61321 ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga acggcgattt
    61381 cctgatttag ctgggcaata ttatctttta actgaacgtt gaggctcaaa tcgcgctcgc
    61441 gcatctcctc cagtttttgc accagccgtg acagacgttg tcgtgactcc tccagttgct
    61501 cgacgaccac cgatagaaag tagaccgccc acggcgtaat cagcaaacca aagaagatag
    61561 aacgaataac atcaatgctt tcgacctgac catgcagcac catggttacc gccatttgta
    61621 ccacaatggc aagaacgacg agggccagcg ccagcaacat tgagaagcgc accagaccta
    61681 acttcatcat caggtcaaca taatactgcg ccagcagacg aatttgcttc attagggaat
    61741 tccttcacga caacctgccc caataatacc caattctgac agttacgtta taaattgtgt
    61801 gagaaatgcg gaattcccct caacgaccta ctccgttgga ggtatccacg gacgttccag
    61861 cgccgatccc tgtacgccat gttcattcag atagcgatcc agctcgacca ttcccgtcca
    61921 tcgattttcg caccacagag gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg
    61981 gtggtagatc acttccggtg gcgtatggcg aatcatttct ccggcagtga gcgtgtaatc
    62041 ctccagttca ataccgttca aacgccccgc ttcccaggct ttcgccataa tgctgccttt
    62101 cacaatatgc agcggatgca gctttatgcc gtccacgccg gtttcaacca ctcgctccag
    62161 cgtttgcaaa cattcagcct gcccttcgcc aggcaggccg acaattaagt gggaacacac
    62221 cttcagcccg cgctgacgtg ccagctgggt tgtacgctga taacaggcaa aatcatggcc
    62281 gcggttgatg cgatgcagtg ttttgtcgtg ggcggtttgt agccccagct ccagccacac
    62341 ttcgtaaccc tggtccttat attcgcaaag cagatccagc accgcatccg gcacgcagtc
    62401 cgggcgggta ccaacacaca aaccgacaat attggcctgg ctcaccgcct gctgatacat
    62461 agaacgcagc acctgaactt ccgcaaaggt gctggtatac gcctgaaagt aggccagata
    62521 gcgtttagcg cggttaacta aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg
    62581 ctgctgcgct tcatcggcaa acgaggcaac attacagaat gtgcagccac cacgcccgat
    62641 ggtaccgtca cggttagggc agctaaaacc gccgtgtagc gtcagcttat gcaccttttg
    62701 cccataacga cgggtgagat caccaccaaa catattgact aatttctgta actgcataat
    62761 ctgatagacc gcgccttgaa aagaggccaa agcctgccat ttttagctca attcggcgat
    62821 gacctggatc aatcgtcccg acccgctttt atcaactgca taatcaatca aaattaccga
    62881 aatttcatgc ataatcacat aaatcacttt tgcttatctt gtgtcagatt tttttatctc
    62941 cttatggatt ttaggcaaaa acagtagcat gaaacgtcat taccaattaa ggcagtataa
    63001 aatgctggtt ttctcgtcag ttcaaggcag gataagggtt aacacgcctt tatgacagtc
    63061 aggaattgac tgtttctcta acgacttccc ttttagcctt aaagataaaa tcaattttaa
    63121 tttcagtaat ttaataaaga attttgcgct aaagcacatt tctgtaccaa taagcttgcc
    63181 atttgacctg tatcagcttt cccgataagt tggaaatccg ctggaagctt tctggatgag
    63241 cagcctgctc atcatattta tgcagtaatt gagatcccct cttcaccgta ttaaccgatg
    63301 cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc
    63361 acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag agcctggggg
    63421 aggttcacga tatgttgtac gataaatccc ttgagaggga taactgtggt ttcggcctga
    63481 tcgcccacat agaaggcgaa cctagccaca aggtagtgcg tactgcaata cacgcactgg
    63541 cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa aaccggcgac ggttgcggct
    63601 tgctgttaca aaaaccggat cgcttttttc gcatcgttgc gcaggagcgc ggctggcgtt
    63661 tagcaaaaaa ctacgctgtc gggatgctct tcctgaataa agatcctgaa ctcgccgctg
    63721 ccgcacgccg catcgttgaa gaagagctgc aacgcgaaac cttgtcgatt gtgggctggc
    63781 gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct ctgccccaca
    63841 ttgagcaaat tttcgttaac gctccggcag gctggcgtcc gcgcgatatg gagcgccgtc
    63901 tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac ttctacgtct
    63961 gtagcctgtc gaatctggtg aacatctata aaggtctgtg tatgccggcg gatctgccgc
    64021 gcttttatct ggatcttgcg gacctgcgtc tggaatcggc catctgcctg ttccaccagc
    64081 gcttctccac taacaccgta ccgcgctggc cgctggcgca gccgttccgc tacctggcgc
    64141 ataacggtga aatcaacacc atcaccggta accgccaatg ggcgcgtgcg cgtacctata
    64201 aattccagac gccgcttatc cctgatctgc acgacgccgc accgttcgtc aatgaaaccg
    64261 gctctgactc cagttcgatg gataacatgc tggaactgct gctggcaggc ggaatggata
    64321 tcatccgcgc catgcgtctg ttagtgccac ccgcctggca gaacaacccg gatatggacc
    64381 cggaactgcg tgccttcttt gactttaact ccatgcatat ggagccgtgg gatggcccgg
    64441 cgggcatcgt gatgtccgac ggtcgcttcg ctgcctgtaa cctcgaccgt aacggtctgc
    64501 gtccggcacg ctacgtcatt accaaagaca agctcatcac ctgcgcctct gaagtcggta
    64561 tctgggatta tcagcctgac gaagtggtcg aaaaaggccg cgtcgggcca ggcgaactga
    64621 tggtgatcga cacccgcagt gggcgtattc tgcactcggc agaaaccgat gacgatctga
    64681 aaagccgcca tccatataaa gagtggatgg agaaaaacgt ccgccgactg gtaccgtttg
    64741 aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg cttgccagct
    64801 accagaaaca gtttaactac agcgcggaag agctggactc cgtaattcgc gtactgggcg
    64861 aaaacggtca ggaagcggtc ggttcgatgg gcgatgatac cccattcgcc gtgctctcca
    64921 gtcagccgcg cattatttac gactacttcc gccagcagtt tgcccaggtg actaacccgc
    64981 caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc caccagtatc ggtcgtgaaa
    65041 tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt aagctttaaa tcgccgattc
    65101 tgctctactc cgatttcaaa cagctcacga cgatgaaaga ggagcactac cgcgcagata
    65161 cgctggatat cacctttgac gtcactaaaa ccacgctcga agcgacagtc aaagagctgt
    65221 gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgct gctggtgctc tccgaccgga
    65281 atatcgctaa agatcgcctg ccggttccag ccccgatggc ggttggcgcg atccagaccc
    65341 gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc gccagcgccc
    65401 gcgatccgca ccacttcgcc gtgttgctgg gcttcggcgc gacggctatt tatccatacc
    65461 ttgcctatga aacgctgggc cgcctggtag acacccatgc gattgccaaa gattatcgta
    65521 ccgtgatgct caactaccgt aacggcatca acaaaggctt gtacaaaatc atgtccaaaa
    65581 tgggcatctc caccatcgcc tcttaccgct gctcgaaact gtttgaagcg gtcggtctgc
    65641 acgatgatgt agtgggcctg tgcttccagg gggcggtcag ccgcattggt ggggcgagct
    65701 ttgaagactt ccagcaggat ctgctgaacc tgtcgaaacg tgcctggctg gcgcgtaagc
    65761 ccatcagcca gggcggcctg ctgaaatacg tccacggcgg cgaataccac gcttacaacc
    65821 cggacgtggt gcgcacgctg caacaggcgg tacaaagcgg cgagtacagc gactatcagg
    65881 aatacgcgaa gctggttaat gagcgtccgg caaccacgct gcgcgatctg ctggcaatta
    65941 cgccgggtga aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa ctgtttaaac
    66001 gctttgatac cgccgcgatg tctatcggcg cgttaagccc ggaagcccac gaggcgctgg
    66061 cggaagcgat gaatagcatc ggcggtaact cgaactccgg tgaaggcggc gaagacccgg
    66121 cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt ggcttctggt cgcttcggcg
    66181 ttactccggc gtatctggtc aatgccgacg tcattcagat taaagtcgcc cagggcgcga
    66241 agccgggcga aggcggtcag ttgccgggcg acaaagtcac tccttacatc gccaaactgc
    66301 gctattcggt gcccggagtg acgctgatct ccccgccgcc gcaccacgat atctactcta
    66361 tcgaggactt agcgcagctc attttcgacc tcaagcaggt taacccgaaa gcgatgatct
    66421 ccgtgaagct ggtttccgaa ccgggcgtag gcaccatcgc gactggcgtg gcaaaagctt
    66481 atgccgattt aatcaccatc gcaggctatg acggtggcac cggcgcaagc ccgctgtcgt
    66541 cagtgaaata cgcaggttgc ccgtgggagc tggggcttgt tgaaacccag caggcgctgg
    66601 ttgccaatgg tctgcgtcat aagatccgtt tgcaggtcga tggcggcctg aaaaccggtg
    66661 ttgatatcat caaagcggcg attctcggcg cagaaagctt cggcttcggc actggcccga
    66721 tggtggcgct cggctgtaaa tatctacgta tttgccatct gaacaactgc gcaacgggtg
    66781 tagcaactca ggatgacaaa ctgcgtaaga accactatca cggcctgccg ttcaaggtta
    66841 ccaattactt tgaatttatt gcccgtgaaa cccgcgagct gatggcacag ctgggcgtaa
    66901 cgcgtctggt ggatctgatt ggtcgcaccg acctgctgaa agaactggac ggtttcaccg
    66961 ccaaacagca aaaactggcg ctgtcgaagc tgctggagac cgccgaaccg catccaggta
    67021 aggcactcta ctgcaccgaa aacaacccac cgtttgataa cggcctgctg aacgcgcagt
    67081 tgctgcaaca ggcgaaaccg tttgtcgatg aacgccagag caaaaccttc tggttcgata
    67141 ttcgcaacac cgaccgttct gtcggcgcgt cgctttcagg ctatatcgcc cagacgcacg
    67201 gcgatcaggg gctggcagcc gatcctatca aagcgtactt caacggcacc gcaggccaga
    67261 gtttcggtgt gtggaacgcg ggcggcgtgg aactgtacct gaccggggat gccaacgact
    67321 atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg tcctccggtt ggttccgcct
    67381 tccgcagcca tgaagcaagt attatcggca acacctgcct gtatggcgcg accggtggtc
    67441 gtctgtatgc cgcaggccgc gcgggtgaac gtttcgg
//
LOCUS       DAEVZA010000007        66043 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEVZA010000007 DAEVZA010000000
VERSION     DAEVZA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.007"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..601)
                     /gene="recF"
                     /locus_tag="LAF92_000503"
     CDS             complement(<1..601)
                     /gene="recF"
                     /locus_tag="LAF92_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBI9789562.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIA"
     gene            complement(601..1701)
                     /gene="dnaN"
                     /locus_tag="LAF92_000504"
     CDS             complement(601..1701)
                     /gene="dnaN"
                     /locus_tag="LAF92_000504"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBI9789563.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(1706..3109)
                     /gene="dnaA"
                     /locus_tag="LAF92_000505"
     CDS             complement(1706..3109)
                     /gene="dnaA"
                     /locus_tag="LAF92_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBI9789564.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            3406..3486
                     /locus_tag="LAF92_000506"
     CDS             3406..3486
                     /locus_tag="LAF92_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789565.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            3716..3856
                     /gene="rpmH"
                     /locus_tag="LAF92_000507"
     CDS             3716..3856
                     /gene="rpmH"
                     /locus_tag="LAF92_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBI9789566.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            3873..4232
                     /gene="rnpA"
                     /locus_tag="LAF92_000508"
     CDS             3873..4232
                     /gene="rnpA"
                     /locus_tag="LAF92_000508"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBI9789567.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            4196..4453
                     /gene="yidD"
                     /locus_tag="LAF92_000509"
     CDS             4196..4453
                     /gene="yidD"
                     /locus_tag="LAF92_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBI9789568.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            4456..6102
                     /gene="yidC"
                     /locus_tag="LAF92_000510"
     CDS             4456..6102
                     /gene="yidC"
                     /locus_tag="LAF92_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBI9789569.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            6208..7572
                     /gene="mnmE"
                     /locus_tag="LAF92_000511"
     CDS             6208..7572
                     /gene="mnmE"
                     /locus_tag="LAF92_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBI9789570.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            7815..7889
                     /gene="tnaC"
                     /locus_tag="LAF92_000512"
     CDS             7815..7889
                     /gene="tnaC"
                     /locus_tag="LAF92_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBI9789571.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            8110..9525
                     /gene="tnaA"
                     /locus_tag="LAF92_000513"
     CDS             8110..9525
                     /gene="tnaA"
                     /locus_tag="LAF92_000513"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBI9789572.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            9616..10863
                     /gene="tnaB"
                     /locus_tag="LAF92_000514"
     CDS             9616..10863
                     /gene="tnaB"
                     /locus_tag="LAF92_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBI9789573.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            10995..12170
                     /gene="mdtL"
                     /locus_tag="LAF92_000515"
     CDS             10995..12170
                     /gene="mdtL"
                     /locus_tag="LAF92_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBI9789574.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIADDLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            12145..13104
                     /gene="yidZ"
                     /locus_tag="LAF92_000516"
     CDS             12145..13104
                     /gene="yidZ"
                     /locus_tag="LAF92_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBI9789575.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            13261..14010
                     /locus_tag="LAF92_000517"
     CDS             13261..14010
                     /locus_tag="LAF92_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBI9789576.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDMQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            14032..14598
                     /gene="yieF"
                     /locus_tag="LAF92_000518"
                     /gene_synonym="chrR"
     CDS             14032..14598
                     /gene="yieF"
                     /locus_tag="LAF92_000518"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBI9789577.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQVLVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(14652..15989)
                     /gene="adeP"
                     /locus_tag="LAF92_000519"
     CDS             complement(14652..15989)
                     /gene="adeP"
                     /locus_tag="LAF92_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBI9789578.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            16156..16821
                     /gene="yieH"
                     /locus_tag="LAF92_000520"
     CDS             16156..16821
                     /gene="yieH"
                     /locus_tag="LAF92_000520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBI9789579.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            16888..17361
                     /locus_tag="LAF92_000521"
     CDS             16888..17361
                     /locus_tag="LAF92_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBI9789580.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            17410..17997
                     /locus_tag="LAF92_000522"
     CDS             17410..17997
                     /locus_tag="LAF92_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBI9789581.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(18059..18781)
                     /locus_tag="LAF92_000523"
     CDS             complement(18059..18781)
                     /locus_tag="LAF92_000523"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBI9789582.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(18796..19965)
                     /locus_tag="LAF92_000524"
     CDS             complement(18796..19965)
                     /locus_tag="LAF92_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBI9789583.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(19992..21608)
                     /gene="bglH"
                     /locus_tag="LAF92_000525"
     CDS             complement(19992..21608)
                     /gene="bglH"
                     /locus_tag="LAF92_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBI9789584.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDVALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(21694..23088)
                     /gene="bglB"
                     /locus_tag="LAF92_000526"
     CDS             complement(21694..23088)
                     /gene="bglB"
                     /locus_tag="LAF92_000526"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBI9789585.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(23107..24984)
                     /gene="bglF"
                     /locus_tag="LAF92_000527"
     CDS             complement(23107..24984)
                     /gene="bglF"
                     /locus_tag="LAF92_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBI9789586.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(25118..25954)
                     /gene="bglG"
                     /locus_tag="LAF92_000528"
     CDS             complement(25118..25954)
                     /gene="bglG"
                     /locus_tag="LAF92_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBI9789587.1"
                     /translation="MNIQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(26240..26965)
                     /gene="phoU"
                     /locus_tag="LAF92_000529"
     CDS             complement(26240..26965)
                     /gene="phoU"
                     /locus_tag="LAF92_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBI9789588.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(26980..27753)
                     /gene="pstB"
                     /locus_tag="LAF92_000530"
     CDS             complement(26980..27753)
                     /gene="pstB"
                     /locus_tag="LAF92_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBI9789589.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(27844..28734)
                     /gene="pstA"
                     /locus_tag="LAF92_000531"
     CDS             complement(27844..28734)
                     /gene="pstA"
                     /locus_tag="LAF92_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBI9789590.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(28734..29693)
                     /gene="pstC"
                     /locus_tag="LAF92_000532"
     CDS             complement(28734..29693)
                     /gene="pstC"
                     /locus_tag="LAF92_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBI9789591.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(29780..30820)
                     /gene="pstS"
                     /locus_tag="LAF92_000533"
     CDS             complement(29780..30820)
                     /gene="pstS"
                     /locus_tag="LAF92_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBI9789592.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(31068..32141)
                     /locus_tag="LAF92_000534"
     CDS             complement(31068..32141)
                     /locus_tag="LAF92_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9789593.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(32152..34674)
                     /locus_tag="LAF92_000535"
     CDS             complement(32152..34674)
                     /locus_tag="LAF92_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI9789594.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(34699..35430)
                     /locus_tag="LAF92_000536"
     CDS             complement(34699..35430)
                     /locus_tag="LAF92_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBI9789595.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(35478..36050)
                     /locus_tag="LAF92_000537"
     CDS             complement(35478..36050)
                     /locus_tag="LAF92_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HBI9789596.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            complement(36352..38181)
                     /gene="glmS"
                     /locus_tag="LAF92_000538"
     CDS             complement(36352..38181)
                     /gene="glmS"
                     /locus_tag="LAF92_000538"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBI9789597.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(38343..39713)
                     /gene="glmU"
                     /locus_tag="LAF92_000539"
     CDS             complement(38343..39713)
                     /gene="glmU"
                     /locus_tag="LAF92_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBI9789598.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     ELRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(40064..40483)
                     /gene="atpC"
                     /locus_tag="LAF92_000540"
     CDS             complement(40064..40483)
                     /gene="atpC"
                     /locus_tag="LAF92_000540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBI9789599.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(40504..41886)
                     /gene="atpD"
                     /locus_tag="LAF92_000541"
     CDS             complement(40504..41886)
                     /gene="atpD"
                     /locus_tag="LAF92_000541"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBI9789600.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(41913..42776)
                     /gene="atpG"
                     /locus_tag="LAF92_000542"
     CDS             complement(41913..42776)
                     /gene="atpG"
                     /locus_tag="LAF92_000542"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBI9789601.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(42827..44368)
                     /gene="atpA"
                     /locus_tag="LAF92_000543"
     CDS             complement(42827..44368)
                     /gene="atpA"
                     /locus_tag="LAF92_000543"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBI9789602.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(44381..44914)
                     /gene="atpH"
                     /locus_tag="LAF92_000544"
     CDS             complement(44381..44914)
                     /gene="atpH"
                     /locus_tag="LAF92_000544"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBI9789603.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(44929..45399)
                     /gene="atpF"
                     /locus_tag="LAF92_000545"
     CDS             complement(44929..45399)
                     /gene="atpF"
                     /locus_tag="LAF92_000545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBI9789604.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(45461..45700)
                     /gene="atpE"
                     /locus_tag="LAF92_000546"
     CDS             complement(45461..45700)
                     /gene="atpE"
                     /locus_tag="LAF92_000546"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBI9789605.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(45747..46562)
                     /gene="atpB"
                     /locus_tag="LAF92_000547"
     CDS             complement(45747..46562)
                     /gene="atpB"
                     /locus_tag="LAF92_000547"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBI9789606.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(46571..46951)
                     /gene="atpI"
                     /locus_tag="LAF92_000548"
     CDS             complement(46571..46951)
                     /gene="atpI"
                     /locus_tag="LAF92_000548"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBI9789607.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(47568..48191)
                     /gene="rsmG"
                     /locus_tag="LAF92_000549"
     CDS             complement(47568..48191)
                     /gene="rsmG"
                     /locus_tag="LAF92_000549"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBI9789608.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(48255..50144)
                     /gene="mnmG"
                     /locus_tag="LAF92_000550"
     CDS             complement(48255..50144)
                     /gene="mnmG"
                     /locus_tag="LAF92_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBI9789609.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(50523..50966)
                     /gene="mioC"
                     /locus_tag="LAF92_000551"
     CDS             complement(50523..50966)
                     /gene="mioC"
                     /locus_tag="LAF92_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBI9789610.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(51056..51514)
                     /gene="asnC"
                     /locus_tag="LAF92_000552"
     CDS             complement(51056..51514)
                     /gene="asnC"
                     /locus_tag="LAF92_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBI9789611.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            51666..52658
                     /gene="asnA"
                     /locus_tag="LAF92_000553"
     CDS             51666..52658
                     /gene="asnA"
                     /locus_tag="LAF92_000553"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBI9789612.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRHLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(52663..54114)
                     /gene="viaA"
                     /locus_tag="LAF92_000554"
     CDS             complement(52663..54114)
                     /gene="viaA"
                     /locus_tag="LAF92_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBI9789613.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(54108..55604)
                     /gene="ravA"
                     /locus_tag="LAF92_000555"
     CDS             complement(54108..55604)
                     /gene="ravA"
                     /locus_tag="LAF92_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBI9789614.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            55827..57695
                     /gene="kup"
                     /locus_tag="LAF92_000556"
     CDS             55827..57695
                     /gene="kup"
                     /locus_tag="LAF92_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBI9789615.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            57862..58281
                     /gene="rbsD"
                     /locus_tag="LAF92_000557"
     CDS             57862..58281
                     /gene="rbsD"
                     /locus_tag="LAF92_000557"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBI9789616.1"
                     /translation="MKKGTVLNSDISSMISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            58289..59794
                     /gene="rbsA"
                     /locus_tag="LAF92_000558"
     CDS             58289..59794
                     /gene="rbsA"
                     /locus_tag="LAF92_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBI9789617.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            59799..60764
                     /gene="rbsC"
                     /locus_tag="LAF92_000559"
     CDS             59799..60764
                     /gene="rbsC"
                     /locus_tag="LAF92_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBI9789618.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            60789..61679
                     /gene="rbsB"
                     /locus_tag="LAF92_000560"
     CDS             60789..61679
                     /gene="rbsB"
                     /locus_tag="LAF92_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HBI9789619.1"
                     /translation="MNMKKLATLVSAVALSATVSANVMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            61805..62734
                     /gene="rbsK"
                     /locus_tag="LAF92_000561"
     CDS             61805..62734
                     /gene="rbsK"
                     /locus_tag="LAF92_000561"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBI9789620.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            62738..63730
                     /gene="rbsR"
                     /locus_tag="LAF92_000562"
     CDS             62738..63730
                     /gene="rbsR"
                     /locus_tag="LAF92_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBI9789621.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(63696..65123)
                     /gene="mdtD"
                     /locus_tag="LAF92_000563"
     CDS             complement(63696..65123)
                     /gene="mdtD"
                     /locus_tag="LAF92_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBI9789622.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(65146..65838)
                     /locus_tag="LAF92_000564"
     CDS             complement(65146..65838)
                     /locus_tag="LAF92_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBI9789623.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT        16296 a        16859 c        16249 g        16639 t
ORIGIN      
        1 ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc agcgggatca
       61 gttctttatc ccacgggcgt agctgttcgt aacgtgtcac ctggcgcagc gccgcattgc
      121 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa
      181 agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
      241 cttcaggcgt tatcagctgc atcggcatca ggtgcgccaa ctccgcaacc ttatgcccgt
      301 cagtaccgtc aatacggact ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg
      361 tctcgcgctc ttcgccctgt aatcgcccat ggagaacaaa cgcctcctgc tcatggcgaa
      421 tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt
      481 ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag ccgggagata
      541 aagcgagatc cgcggtttca atgttgcgga aatcgcggat caacaagcgg gtgagggaca
      601 ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat cttcaatctg
      661 cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca gcgcgttcag
      721 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
      781 gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcggtgattt tcagctggtt
      841 ttcgctgaca taaagacgta cgccgcggaa tttctcgtta gagagaatcg ccgcgcgagc
      901 aaacgcctgc ttgagcagat cgcagccagc ttccagatgt ttgtccgggt tcttcggcag
      961 aacgcggcga taatccggga agcgaccatc caccagtttg gaggtgaaga taaagtcgcc
     1021 aacgtgggcg cgaatgttgt tgctgccaat ctgtacgcgc agcggattgt cgccgccgtc
     1081 gagcatacgc atcagttcaa tcacgccttt acgcggtacg atcaccgaat ggcttggcaa
     1141 agattgacca attggcattg aacagaccgc cagacggtgg ccgtcggttg ccacggtgcg
     1201 cagttcttca ccttcggttt caaacagcat accatttaag taatagcgaa cgtcctgatg
     1261 cgccatagaa aactgggtcg cttcaatcag acgcttcatc gttgcctgcg gcagggtaaa
     1321 ttcgacttca ctctgccagt catcgaggtt cgggaaatcc gccgctggca gggtagacag
     1381 cgaaaaacgg ctacgcccgg agcgtaccag catccgttca ccttccagct gcacggcaat
     1441 ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
     1501 cgctcctggc tcgtgtggct gaaccagcgc aacacgtgcc accatttcca tctcgagatc
     1561 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
     1621 tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc
     1681 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa tttgaaaaat
     1741 cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag gcatgaagca
     1801 ccgtcgtgtg gtcacgacca ccaaacgcat cgccaatctc cggcagactg tggttagtca
     1861 gctctttcgc cagcgccatc gccatctggc gcggacgcgc caccgagcgg gatcgacgct
     1921 tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
     1981 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
     2041 cgatggtgat cgcccgtccg gtaaagttgg cattggcaat gacgcggttc agcgcccctt
     2101 ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact tcgcccggca
     2161 aacgaatgtc gttttcgtca gcctttttca tcaggatcgc cacacgggtt tccagctctg
     2221 gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga tcctcaacgc
     2281 cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta ccttccagca
     2341 gggcgttgaa ggtatggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
     2401 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
     2461 tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
     2521 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac
     2581 ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg ttatccgcca
     2641 cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
     2701 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg acgttatccc
     2761 aacctgagcg cgtagaaggc gcagcgcgtt gcggctgcgt ttgcgccacc tgtgcagggg
     2821 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
     2881 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
     2941 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg
     3001 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
     3061 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
     3121 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
     3181 aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
     3241 tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt
     3301 ctcacagatt ttcccgattt atccacagga ctttccagac ctcgctaagt gtaaacgatc
     3361 ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa
     3421 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc
     3481 gtataatcct ccacccggcg cgccatgctg gttttcactg gtgtgaggtc gtacattttc
     3541 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggagtgta
     3601 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
     3661 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
     3721 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
     3781 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
     3841 gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt
     3901 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
     3961 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
     4021 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc
     4081 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
     4141 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
     4201 gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
     4261 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
     4321 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
     4381 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
     4441 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
     4501 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
     4561 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
     4621 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
     4681 ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag
     4741 ccgttccagc tgctggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
     4801 ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa
     4861 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
     4921 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
     4981 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctccac ctttggtcag
     5041 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
     5101 cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtatgagaa atacaagttc
     5161 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcgatg
     5221 ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa caacttctat
     5281 accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc ggtactggtt
     5341 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
     5401 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
     5461 atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc
     5521 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg
     5581 cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag
     5641 cgtctgggtg atgacaaaca gcgtatcagc caggaaatga tggcgctgta caaagctgag
     5701 aaggttaacc cgctgggtgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg
     5761 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
     5821 cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg
     5881 ttcttcattc agaagatgtc gccgaccaca gtgaccgacc cgatgcagca gaagatcatg
     5941 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtgctg
     6001 tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta ccgtggtctg
     6061 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
     6121 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcgca
     6181 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
     6241 acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa
     6301 gttgccgaaa ccgtgctggg taaactacct aagccgcgct acgccgatta tcttccgttt
     6361 aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac
     6421 tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac
     6481 ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc tggtgagttt
     6541 tccgaacgcg cgtttcttaa cgataaactc gacttagccc aggccgaggc gattgccgat
     6601 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
     6661 ttctccgcac gggtcaacca tctggtggaa gccctcaccc acttgcgcat ttacgtcgaa
     6721 gcggcaattg atttccctga tgaagagatc gatttcctct ccgacggcaa aattgaagcc
     6781 cagcttaatg acgtgattgc cgatctcgat gcagtgcgtg ctgaagcacg tcagggtagt
     6841 ttgttgcgcg aagggatgaa ggtggtgatt gccggacgtc ctaacgccgg taaatcgagc
     6901 ctgttaaacg cgctggcggg ccgtgaagca gcaatcgtaa ctgatatcgc cggaaccaca
     6961 cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgatacc
     7021 gctgggctac gtgaagccag tgacgaagtg gaacgtattg gtatcgagcg cgcgtggcag
     7081 gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacaac agacgccgtg
     7141 gatccggcag agatctggcc ggaatttatc gcccgtctgc cagcgaaact gccgatcacc
     7201 gtggtgcgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga agtgaacggt
     7261 cacgcgttaa ttcgtctctc ggcaaggaca ggtgaaggcg tggaggtgct gcgtaaccat
     7321 ctcaaacaga gcatgggctt tgacaccaac atggaaggcg gcttcctggc gcgccgtcgc
     7381 cacctacagg cgctggaaca ggcggcagag catttgcagc aaggtaaagc gcaactgtta
     7441 ggtgcctggg cgggcgaact gctggcggaa gagttgcgcc tggcacagca gaacttaagc
     7501 gaaatcaccg gggaatttac ttctgatgac ctgctggggc ggattttctc cagcttctgt
     7561 attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg cttcgcttca
     7621 ttgttagcac tcctgttatt cctcaaccct ttttttaaac attaaaattc ttacgtaatt
     7681 tataatctta aaaaaaagca tttaatattg ctccccgaac gattgtgatt cgattcacat
     7741 ttaaacaatt ttagaataga caaaattctt gagtattata ctgtagcctc gtgtctttcg
     7801 aggataagtg cattatgaat atcttacata tatgtgtgac ctcaaaatgg ttcaatatcg
     7861 acaataagat tgtcgatcac cgcccttaat ttacccttct gtagccatca ccagagccaa
     7921 accgattaga ttcaatgtga acgattagtt tgctatatct taattttgcc ttttgcaaag
     7981 gccatctctc gtttatttac ttattttagt aaatgatggt gcttgtatat atacatggcg
     8041 aattaatgga tattgcagat gtaatattca cagggatcac tgtaattaaa ataaatgaag
     8101 gattatgtaa tggaaaactt taaacatctc cctgaaccgt tccgcattcg tgttattgag
     8161 ccagtaaaac gtactactcg cgcttatcgt gaagaagcaa ttattaaatc cggtatgaac
     8221 ccgttcctgc tggatagcga agatgtgttt atcgatttac tgaccgacag cggcaccggg
     8281 gcggtaaccc aaagtatgca ggcagcgatg atgcgcggcg acgaagccta cagcggcagc
     8341 cgcagctact atgcgttagc cgagtcagtg aaaaatatct ttggttatca atatactatt
     8401 ccgactcacc agggccgtgg cgcagagcaa atctatattc cggtactgat taaaaaacgc
     8461 gagcaggaaa aaggcctgga tcgcagcaaa atggtggcat tctctaacta tttctttgat
     8521 accacgcagg gccatagcca gattaacggc tgtaccgtgc gtaacgtcta tatcaaagaa
     8581 gccttcgata cgggcgtgcg ttacgacttt aaaggcaact ttgacctcga aggattagaa
     8641 cgcggtattg aagaagttgg cccgaataac gtgccgtata tcgttgcaac catcaccagt
     8701 aactctgcag gtggtcagcc ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg
     8761 aagaaatacg atattccggt ggtaatggac tccgcacgct ttgctgaaaa cgcctatttc
     8821 atcaagcagc gtgaagcaga atacaaagac tggaccatcg agcagatcac ccgcgaaacc
     8881 tacaaatatg ccgatatgct ggcgatgtcc gccaagaaag atgcgatggt accgatgggc
     8941 ggcttgctgt gcatgaaaga cgacagcttc tttgatgtgt acaccgagtg cagaaccctt
     9001 tgcgtggtgc aggaagggtt cccgacatat ggcggcctgg aaggcggcgc gatggagcgt
     9061 ctggcggtag gtctgtatga cggcatgaat ctcgactggc tggcttatcg tatcgcgcag
     9121 gtacagtatc tggtcgatgg tctggaagag attggcgttg tctgccagca ggcgggcggt
     9181 cacgcggcat tcgttgatgc cggtaaactg ctgccgcata tcccggcaga ccagttcccg
     9241 gcacaggcgc tggcgtgcga gctgtataaa gtcgccggta tccgtgcggt agaaattggc
     9301 tctttcctgt taggccgcga tccgaaaacc ggtaaacaac tgccatgccc ggctgaactg
     9361 ctgcgtttaa ccattccgcg cgcaacatat actcaaacac atatggactt cattattgaa
     9421 gcctttaaac atgtgaaaga gaacgcggcg aatattaaag gattaacctt tacctacgaa
     9481 ccaaaagtat tgcgtcactt caccgcaaaa ctgaaagaag tttaattaat actacagagt
     9541 ggctataagg atgttagcca ctctcttacc ctacatcctc aataacaaaa atagccttcc
     9601 tctaaaggtg gcatcatgac tgatcaagct gaaaaaaagc actctgcatt ttggggtgtt
     9661 atggttatag caggtacagt aattggtgga ggtatgtttg ctttacctgt tgatcttgcc
     9721 ggtgcctggt ttttctgggg tgcctttatc cttatcattg cctggttttc aatgcttcat
     9781 tccgggttat tgttattaga agcaaattta aattatcctg tcggctccag ttttaacacc
     9841 atcaccaaag atttaatcgg taacacctgg aacattatca gcggtattac cgttgccttc
     9901 gttctctata tcctcactta tgcctatatc tctgctaatg gtgcgatcat tagtgaaacg
     9961 atatcaatga atttgggcta tcacgctaat ccacgtattg tcgggatctg cacagccatt
    10021 ttcgttgcca gcgtattgtg gataagttcg ttagccgcca gtcgcattac ctcattgttc
    10081 ctcgggctga agattatctc ctttgtgatc gtgtttggtt ctttcttctt ccaggtcgat
    10141 tactccatcc tgcgcgatgc caccagcacc actgcgggaa cgtcttactt cccgtatatc
    10201 tttatggctt tgccggtgtg tctggcgtca tttggtttcc acggcaatat tcccagcctg
    10261 attatttgct atggaaaacg caaagataag ttaatcaaaa gcgtggtatt tggttcgctg
    10321 ctggcgctgg tgatttatct cttctggctc tattgcacga tggggaatat tccgcgcgaa
    10381 agctttaagg cgataatctc ctcaggcggc aacgttgatt cgctggtgaa atcgttcctc
    10441 ggcaccaaac agcacggcat tatcgagttt tgcctgctgg tgttctctaa cttagctgtt
    10501 gccagttcgt tctttggtgt cacgctgggg ttgttcgatt atctggcgga cctgtttaag
    10561 attgataact cccacggcgg gcgtttcaaa accgtgctgt taaccttcct gccacctgcg
    10621 ttgttgtatc tgatcttccc gaacggcttt atttacggga tcggcggtgc cgggctgtgc
    10681 gccaccatct gggcggtcat tattcccgca gtgcttgcaa tcaaagctcg caagaagttt
    10741 cccaatcaga tgttcacggt ctggggcggc aatcttattc cggcgattgt cattctcttt
    10801 ggtataaccg tgattttgtg ctggttcggc aacgtcttta acgtgttacc taaatttggc
    10861 taaatccttc aagaagccag ccattcgctg gcttcttgcc tctcaggaaa tcacttatgt
    10921 ccaaatggca actcgcctga ttctccttca ccacgtatgc tttgcgtcac cttactatca
    10981 ggacgcttta gcccatgtcc cgctttttaa tttgtagttt tgccctggtt ttactttatc
    11041 ccgccgggat tgatatgtac ctcgttggtt taccgcgcat cgccgacgat ctcaatgcca
    11101 gcgaagcgca gttgcatatt gcgttctccg tttatctggc gggcatggca gctgcgatgt
    11161 tatttgccgg taaagtggcc gatcgttcag ggagaaagcc tgtcgccatc cccggcgcgg
    11221 cgctatttat tatcgcttcg gtgttctgtt cactggctga aaccagcacg ttatttcttg
    11281 caggccgatt tctacagggg ttgggcgcag gctgttgtta cgtagtggcg ttcgccattt
    11341 tgcgcgacac gctggatgat cgacgtcggg ctaaagtgct gtcattactc aacggtatta
    11401 cctgcatcat tccggtgtta gcgccagtgc tcggacatct gattatgctt aaattcccgt
    11461 ggcagagtct gttctgggcg atggcaatga tgggcatcgc ggtactgatg ttgtctttgt
    11521 ttattttaaa agaaacgcgc ccagcgtccc ccgctgcttc ggacaaacca cgagaaaata
    11581 gcgagtcgct gcttaatcgg tttttcctca gccgtgttgt tatcaccacc ctcagcgttt
    11641 cggtgatcct cactttcgtc aatacatcgc cggtattgct gatggaaatc atgggttttg
    11701 agcgcggaga atacgccacc attatggcgt tgactgctgg cgtcagcatg accgtttcat
    11761 tctccacgcc atttgcgctg ggaattttta agccacgtac gttgatgatc acctcgcagg
    11821 tgttattcct tgcagcgggg atcacccttg ccgtttcacc ttcccatgcg gtttctctgt
    11881 ttggtatcac gctgatttgc gccggtttct cggtaggttt tggcgtagcg atgagtcagg
    11941 cgttaggacc attttcatta cgcgcgggcg tagccagctc gaccttaggt attgcgcagg
    12001 tttgcggttc gtcactgtgg atttggctgg cagcggtggt tggtatcggc gcatggaata
    12061 tgctgatcgg gattctgatt gcctgtagca tagtgagcct gttgctgatt atgttcgtcg
    12121 cgcctggacg ccccgttgcc gctcatgaag aaatccatca ccacgcttga tctcaatctg
    12181 ctgctctgtc tgcaactgct gatgcaggag cgcagcgtga ccaaagcggc gaagcggatg
    12241 aacgtgaccc cttcagcggt gagtaagtcg ctggcaaagt taagagcgtg gtttgacgac
    12301 ccgctctttg tgaactcacc gctgggcctg tcgcccacac cgctaatggt cagcatggag
    12361 caaaatctgg cggaatggat gcaaatgagc aatctgctgc tggataaacc gcaccaccag
    12421 acaccgcgcg gcctgaagtt tgagttggcg gcggaatcgc cgctgatgat gatcatgctt
    12481 aatgcgctgt cgaaacggat ctaccaacgt tacccgcagg cgaccatcaa actacgtaac
    12541 tgggattacg attccttaga tgccattact cgtggtgaag tggatattgg tttttccggt
    12601 cgcgaaagcc atcctcgctc gcgggagctg ttaagctcgc taccgttagc cattgattat
    12661 gaagtgctgt ttagtgatgt accctgcgtc tggttacgca aagatcatcc ggcactgcat
    12721 gaaacgtgga atctggacac ctttttacgt tatccgcata tcagcatttg ctgggaacag
    12781 agcgacacct gggcgctgga caatgtgtta caggagctgg ggcgcgaacg cacgattgct
    12841 atgagcctgc cggaatttga gcagtcactg tttatggcag cgcaacccga caatctgcta
    12901 ctggcgaccg cgccgcgcta ctgtcagtac tacaatcaac tccatcaact gccgttggtt
    12961 gctcttcctc tcccgtttga cgaaagccag caaaaaaagc tggaagttcc ttttaccctg
    13021 ctgtggcata aacggaacag ccataatccg aagatcgtct ggttacggga aaccattaaa
    13081 aacctttacg cgtcgatggc ataaccgaat cgtatgaaac gcgacccaat ttcacaataa
    13141 aatgtaaaaa agttgtaata agcttgtctg aatcgaactt ttagccgctt tagtctgtcc
    13201 atcattccag taaatgatta ctcttgtatt cataatggac cattaagcat ggagcgaaaa
    13261 atggcgactc actttgcccg agggatttta acggaaggac atctgatttc tgttcgtctc
    13321 ccctcccagt gtcatcaaga agcccgaaac attccccctc atcgtcaaag ccgttttctg
    13381 gcgtccagag gtttactcgc agaactgatg ttcatgctgt atggcattgg cgaattgccg
    13441 gaaatcgtca ccctgccgaa aggtaaaccg gttttcagtg ataaaaattt gccttcgttt
    13501 tctatttcct atgccgggaa tatggttggc gtggcgttaa caaccgaagg tgaatgtggc
    13561 ctcgatatgg aactacagcg tgcgacgcgc gggtttcata gcccacacgc gcccgataac
    13621 cacacctttt ccagcaatga atcgctatgg atcagtaaac aaaacgatcc taacgaagcg
    13681 cgggcgcagc tcatcacgct gcgccgaagc gtgctaaaac taaccggtga tgttttgaat
    13741 gacgatccgc gcgatatgca gctgctgccc attgccggac gcctgaaatg tgctcatgta
    13801 aatcatgtag aagcgttatg cgacgcggag gacgtgctag tgtggtccgt ggcggtcacg
    13861 cccgcgattg aaaagctcag tgtctgggag ttagatggca aacacagctg gaaaagcctg
    13921 ccagatattc acagccgcgc caacaatcct accagccgga tgatgcgttt tgcccagctc
    13981 tctaccgtga agtctttttc gccaaattga tagacaacca caggagtcat tatgtctgaa
    14041 aaattgcagg tggttacgtt actggggagc ctgcgcaaag gctcatttaa tggcatggtt
    14101 gcacgtaccc tgccgaaaat tgctccggcg agcatggaag tcaatgcgtt accatccatt
    14161 gccgacattc ccttgtatga cgctgacgta cagcaggaag aaggttttcc agcaacggtt
    14221 gaagctctgg cggaacagat ccgtcaggct gacggtgtgg tgatcgtcac gccggaatat
    14281 aactactcgg taccgggtgg gctgaaaaat gccatcgact ggctttcccg cctgccggat
    14341 caaccgctgg caggtaaacc ggtgttgatt cagaccagct caatgggcgt gattggcggc
    14401 gcgcgctgtc agtatcacct gcgccaggtt ctggttttcc tcgatgcaat ggtgatgaac
    14461 aagccggaat ttatgggcgg cgtgattcag aacaaagttg atccgcaaac cggagaagtg
    14521 attgatcagg gtacgctgga ccacctgacc gggcaattga ccgcatttgg tgagtttatt
    14581 cagcgagtta agatctaaat aaaaaacccg ccagtaatca tgcatggcgg gtttttaatg
    14641 cgctatcgat tttaatgagc gtcgataaat acaatcttca ggataaacat cagcgcaacg
    14701 atgattacgc acgggctaag gtcacgcaga cgcccggtac caatcttcat cacgcagtag
    14761 gagataaagc ccagcgcaat accttcggta atcgaaaagc tgaacggcat catcacggcg
    14821 gtaataaacg ccggaacaga ttcagtaaga tcctgccagt tcacgcgagc aagacttgag
    14881 gtcatcaaca cgccaacgta gatcaacgcg ccagctgcag cgtagcctgg caccattccc
    14941 gccagcggcg acagaaagat aaccagcagg aacagcagac caacaaccac tgccgtcaga
    15001 ccggtacgac caccaacaga aacgccggaa gaagactcaa tatacgccgt aacagaagaa
    15061 gtaccgataa acgaaccggt caccgaagag atactgtcga catacagcgc ctgcttcatg
    15121 cgcgggaatt tccccttctc atctgccaga cctgctttat cggtcacacc aatcagcgta
    15181 ccggaggagt caaacaggtt gaccagcata aaagagaaaa tcacccctgc caggccgagg
    15241 ttaaacgacc cggctaaatc tacatgaccc acgacagtca ttacgctcgg cggcgcagaa
    15301 acgatgccat tatagtggac atcacccagc atccagccca gcagcgttgt caccacgatg
    15361 gaaaccagca ccgccgcgtg aatgttgcgc gaggccagaa tggcgatgat gaagaaaccg
    15421 aggatcccca gcagcacgct gtgagaagtc agattaccga tgctgaccag cgtttccggg
    15481 ttagcgacaa tcacacctgc gtttttcagc cccatcatgc caatgaacag accgataccg
    15541 ctggtaatac ccacacgcag actcaccgga atgttggcta tcatccagta gcgaacgcgg
    15601 aaaatcgtca gtaacagcag acctatcgcg ccccagaaga ttgcgcccat cccgacctgc
    15661 cacggcaagc ccatcgcctg tacaacgaca aaagcgaaga acgcattcag gcccatagcg
    15721 ggtgccagtg caactggcag gttagcaaac agtcccatca taatactgcc gaatgcagcg
    15781 atcagacagg tagtgacgaa gacggcgctg gtatccatgc cagcaacgcc aagaatttgc
    15841 gggttaacaa aaacgatgta aaccatcgtc aggaaggtgg taaaaccggc gatcacttcg
    15901 gtccgtgccg tcgtgccatg ttcgcgcagt ttaaacacgc gttccagcat cccctggcca
    15961 gaagcctggg ttgtgtgttg ttgactcatt atctatttcc gaacaaggag ggaaaatccg
    16021 tcgctatcgt ataccaaaat gagacaatat ggacggctga agataacttt ttttcattgt
    16081 atttgcttat acggcaacga ttgcgtcatg cgaattggca tgacgaaaac gttaaactaa
    16141 cgataaagga aagacatgtc ccagatagaa gcggtatttt tcgactgcga cggtacgctg
    16201 gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt
    16261 acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc
    16321 gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga acacgtttac
    16381 cgtgcagaag tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctggagcg
    16441 ctcctgtcag cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg
    16501 cagcattcta tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc
    16561 tacgatattc agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg
    16621 aatgtaaatg tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt
    16681 atcgacgcag gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt
    16741 cacccgaaag tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt
    16801 ggttgggata ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg
    16861 ctcaaaggta agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga
    16921 ctttattttg cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc
    16981 ttcgcaaaag aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta
    17041 ttgtcgatta ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg
    17101 ccggaaaaga tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc
    17161 gttatcaccg agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag
    17221 ttgtttgaaa acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg
    17281 gcaggtgtgg tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct
    17341 gaaacagact ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag
    17401 gatgttacta tgactcaaaa tatcaggccg ttaccccaat tcaaatatca tcccaagcca
    17461 ctggaaacag gcgcatttga acaggataaa accgtagagt gcgattgctg tgaacaacag
    17521 acgtcagttt attactcggg tcccttttat tgcgttgatg aagttgaaca tctctgtccg
    17581 tggtgtattg cggacggttc tgctgctgaa aagtttgcag gtagttttca ggatgatgcc
    17641 agcatagaag gtgttgaatt tgagtatgat gaagaggacg aatttgccgg tattaagaac
    17701 acatatcctg atgaaatgct gaaagagttg gttgaacgca cgccaggtta tcatggatgg
    17761 caacaggaat tctggcttgc gcattgtggc gatttctgtg cttttatcgg ctatgtgggc
    17821 tggaatgata taaaagatcg cctcgatgaa tttgccaacc ttgaagaaga ttgtgagaat
    17881 ttcggtatta gaaattctga tctagctaaa tgcctgcaaa agcgtggtga ttgtcagggt
    17941 tatctcttcc gctgtctcca ctgcggcaag ctgagactgt ggggtgattt ttcgtagtta
    18001 tttaaataat gagaacaggc cggagcgtaa ttcatacatc cggccttatt tcttcagctt
    18061 aaccgagcgc taattctgcg gcagcggctt tatcagcgat taccatcagc gaaggatgca
    18121 gctgcaacac ggaagctgga acatcttcgg taaccggacc ttgcagcaca tttttcagtg
    18181 cctgcgcttt acctgcgcca ctgacaatta tcagcaagtt tttcgccgcc atgatgcttt
    18241 tcggccccat cgtcacgtag ctgtcaggca ctaacgaaaa gtcgccgccc aactcaccat
    18301 gcgccacaat atcaaccatc tctccctgga tcgggaactc cacggtttgc tcatggaaat
    18361 gggtcgtatt cggcaaattg ccacaaaaat gaccatctgc acctaatccc agcaccacca
    18421 aatccaggcc gccttcacgc gccagtttct gatcatgctc gcggtagtta tcaatagtga
    18481 gcttctggat attctcttct ttgatccccg caggggtgaa aaacagatta cgcagattgg
    18541 taatcgttac accttctccc tctttgccgc gaaagggaat ttcatcgaaa ttatagaaat
    18601 agcagttatc ataccagggc ttacctttaa ccagggtggt gaggtattca tacatgcctt
    18661 tgggcgtgct accggcggta attgccaggt taacacgacg cgtcttcgac atataaccaa
    18721 gcagatggtg tgccgcgaca cggctcattt cctggtaatc ttcggtaatg attaatttca
    18781 tcttagtgcc ttattttatt taaataattt ctgtacgaag gctgcatagg caggccgcca
    18841 gacatccatt tcatggttca ggccgggata ttcctggtaa tcaaacttaa tttttttctg
    18901 ctcaagctca gttttcagcc cggcgatatc cttgccggtt acgacatctt tatcaccaac
    18961 gaccacagta aaattacgta gttgctggtt aatagctgcc ggatcgttca gccgggccgc
    19021 gacaccttca tccggtacgg ttgtcgtggt aacaccactg aatgtggcca gccagccaaa
    19081 gctttccaga tgattcattc cggaaaccag cgcctggtac ccgccttgtg aaagccctgc
    19141 cagcgcgcga ccatcggcat ctttacggac attaaaacgc ttgctaatca gcgggataat
    19201 atcgttcatc agttcgcgat ctgccgcttt agcattcagc ggataaaaga ctttacgtct
    19261 ttcctgaggc acgaaatctt cgggaataat gcccttcgca tcggtttcgg tatccgggat
    19321 caccaccagc atcggtttaa ttttcccttc agccagcagg ttatccatga tttgcgggat
    19381 acgcccctga tcgatagcgg aacgtccggt atcaccaaaa ccgtgataga aatagagcac
    19441 tggcaaaggc tcgcccatgc cggtgtatcc tggcggggtc cagacataca tctgacgttc
    19501 agattgcaat gcgttggagt ggtaagttat ggcgatcaaa tcaccatgcg caacagaacg
    19561 cgtatccaga taactgcctg gcaccagaat catactggag ttaacctggc gctgaggctt
    19621 agtcattgcg gtgcctgtat caatgctgcg tacaccatca acattgaaaa aatactcgta
    19681 caggttgcct ttcaggatag gtgtgcgcca cgaccagact cctgcttcgt ctttggtcat
    19741 cgggtgaata ttgtccggaa ccggaacacc caccactaca gagacatttt ttgccccagg
    19801 ggcaaagtag cgaaaggtca cgctgttatc ggcattgacc tgagtcacat actgtttcac
    19861 cgggatagtg ggtgccgggc tggctggcat atccgctgct atggcccact gagcagaaat
    19921 accgctggca atagccagcg ttaaagcagc aatttttata ttcatcgtaa tattcctttc
    19981 attaagttcc attaccacca gatttcagcc tgggcaccga cagcaaactg gtcgtcttta
    20041 ttgtcttcga aggtgaagcc atccagttcg ttttccaggg ctttgatata cgtggcgtag
    20101 aaacgaattt ccggacgcga ggtcaacata ctggtattga ctttaaaggt atggaagagc
    20161 gtggttttat aaccagactc attaaattta ttactattcg catctttgtt ttgctgggtg
    20221 aaatagccca gctcaacacc tgtctggtta tattggtccc aaatataggc cgggcgaaca
    20281 accgcacgaa tagattcgaa atcagagtgt gcgcctgttt cgtagctata aatatcgtta
    20341 ccgaaggagt aaacaatcgc gttagccaca atgaaatgat cgccgatata ggcttcgccc
    20401 tgcgaagtca gacgaactgc tgttccgccg gtgtgatcac cataataacg accattaaag
    20461 gtagtaaatg gacttgcgcc agcataacgg ccaaagttac tggcgataga attattcgcg
    20521 accaggaagg agaattcgtt gaagccacct ttatcaaatt tctgcgttaa agatgtgcca
    20581 aacatccagg tatctttcca gtcataatac ccgttattat cctgattatc tttttccgat
    20641 gcactttcgt ttgcagtgac ataacgaccg ctcaccatta aggtggcttt atcccataac
    20701 gggatatctt tatagcgtaa atcaatggta ttagtattta tctgctgttt gttttgcagg
    20761 ctgcgatcgt agtcatcgac atcttcacga accagcgcga catcaatttt acccggaccg
    20821 actttccagt tttccagacc tacacctgcg gcagcatcgg tacgctgggt tttccagtca
    20881 agcatctgga tttcaatttt cggcgcacca tgtttaccca cccagaaatc agcctctggc
    20941 gcaaagggca ggaaaccttt ggtggtaacg tacatatcgg agaactgcat aaagttctcg
    21001 ccaccggcgt tatcgccaaa ccagccggta gagtactgct gaccaacgtt accatccatc
    21061 atcacaacgg catcaacccg tttgccgttt tcgttgtaga cacgttgttt taactgcaaa
    21121 tcaaaccagc cggagtattc gttaccaaag cggcccagag aaccaatcgc ccatgattta
    21181 ggtgaaccat gagaggcggt cccccaacca gaacggtaat agccgttgta actaaagcca
    21241 atttcatctt tcacaaactt actgaaatct ttcagagtca tggaagaata aatcgagcct
    21301 gtctcactgg catttttctc ttcatttttg accagtacag catcaggttt tgcggcacta
    21361 ctggtcggga acggattggc ggcataccct tgatcattcg tacttacgct acgattcacc
    21421 gtcgctggcg tatacttttt cttctcttca tctttatatt ttttgagttc gctttgcgtt
    21481 tctcttaacg ctttttctaa taactcaagg cgatgttcca ccgttaatga ttcagccaat
    21541 gattgtgcag aaaaggctaa cggtgccatt aataagatgg cagaggtaat aagatttcgt
    21601 ctaaacataa tatcccttta tggtgcaaag aaagaattaa cgtatcgcat ccagactgtt
    21661 ctgaatgcga cgataattaa ggtgctttat tggttatttt tttaatgaca gcccccgcgt
    21721 tttgatcact tctgcatacc atccgaagct tttcttacgc gttcttgtga ggcttccttc
    21781 gccattatca tcacgatcca cataaataaa gccgtagcgc ttagacattt gtgaatgaga
    21841 tgcactgact aaatcaattg gcccccaact ggtgtacccc ataatatcca caccatcggc
    21901 aatcgcttca tttacctgta ccaggtgatc gtttaaatag gcaattcgat aatcgtcctg
    21961 tatcgaacca tccgcttcaa cgctgtcttt tgcgcctaat ccgttctcga caataaataa
    22021 cggtttttga taacgatccc aaagcgtatt taacagaacc cgtaatccaa ccgaatcaat
    22081 ttgccacccc cactctgaac ttttcagatg cggattgggg atcatattca gtatgttgcc
    22141 ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa ccagtcatgt aataactaaa
    22201 agagatgaaa tcgacggtat gttttaaatc ttctgcgtca ctttcagtca tctcaatggt
    22261 gatattgtgg tcgcggaaga aacgctgcat atagccggga tactggccac gcgcctgaac
    22321 atcaccaaag aacatccagc gccggttctc ttccatggcc tgcaacatat cctgtggctg
    22381 gcaggtgagg gggtaaacca gcccaccgag aagcatattg ccgattttcg cttcggggag
    22441 caggctatga caggctttaa ctgcccgcgc actggcaacc agttgatggt ggatagcctg
    22501 ataaacttcc gcctcgccac tctcttctgc cagccccacg cccgtgaatg gctcgtgtaa
    22561 cgacatgttg atttcattaa acgtcagcca taacgccact ttatgttggt agcgagtaaa
    22621 gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgct cgattagccc aaccgccgta
    22681 gtttttcacc agcccatatg gcatttcgta atgggataac gttaccagcg gcttgatccc
    22741 cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct aaccccgctt cattcggttc
    22801 gacttcgtcg ccctgaggga aaattcgcgc ccaggcaatg gaaatacgca gacaggtgaa
    22861 gcccatctcg gcaaataacg cgatatcttc cgggtaacgg tgataaaaat cgatggcgac
    22921 atctttgata ttctctttcc ccaggatgcg cggttccatt tttcccatta cgccatgagg
    22981 ctgtaaatct gaggtcgaga tccctttgcc atcttcctgc caggcacctt ccacctgatt
    23041 ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat gctttcataa ttaactcctt
    23101 ttatcgttag cgaatgatgg ataacagagg ttcacctgcg cttatctgcg ccgtgccgtg
    23161 gggtaatacg tccgtaaaat catcgctatt actgattaat accggcgtcg tcagatcaaa
    23221 tccggcctcg cgaatagcag ggatatcaaa agaaatcagc cgatcgcctg tattgacctt
    23281 gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc agttttacgg tgtcgatacc
    23341 gacatgaatc aggagctcca caccatcatc tgactcaatg ccaatggcgt gtaatgtggc
    23401 gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact tcaccaaccg agggcagaat
    23461 ggcgatacct ttacccaaca ggccactggc aaacgtggta tcagcgacgt gaatgagcgg
    23521 cacaatctct cccgtcatcg gtgaacagat accgccctgc tcaggtggtg taataacctc
    23581 tggtgttttc tcttgcgggg caccctgcgc tggctgacgt ttagcggtga tgaaatgaag
    23641 catcaccgta ccgacaaatg cgcaaccgat ggcaatgaca ccgccaataa cgctggccca
    23701 gacggtgaaa tcaattcccg ttgacgggat ggtttgcatg aaggtgaaaa tacttggcaa
    23761 accaaaggag tagactttcg tttgcgcgta gccaatgatg gtggccccca aagccccact
    23821 gatacaggcg ataacaaagg ggtacttacg cggcagatta acgccatata ccgctggttc
    23881 ggtgatacca aacaggcccg tcaacgccgc tgatcccgcc accacttttt tctgcgcatc
    23941 gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc gccataatgg cgggcattaa
    24001 cagcgggatc atggtgtcgt agcccagcac ggtgaagtta ttgatacata gcggcaccag
    24061 gccccagtgc agtccgaaca tgacgaagat ttgccagaag ccgcccatta ccgcgcctgc
    24121 aaatgcagga accgcctgat aaagccagag ataaccggcg gcaatcagtt cgcttatcca
    24181 ggttgatagc ggccccacca gcagaaaggt gacgggtgtg ataaccatca gacatagcaa
    24241 tggtgtgaag aaatttttga ttgccgacgg taaccacgca ttaagtcggc gttccagaat
    24301 gctgcacaac caggcagaaa aaataatggg aataaccgat gacgagtaat tcaacaatgt
    24361 gaccggaata cccaggaaat ccagccccag ctcatccgct ttttgcccgt tctcgaaagc
    24421 agtcagaatt aatggatgca ctaacgctcc accaatcacc atggcagtaa atggattacc
    24481 gccgaagcgt ttccccgcgg tgtatcccag gattatcggg aagaaccaaa acaaggcatc
    24541 actggcgctg aataaaatta aataagtacc actttgttcg gtcgtccact gaaaagtgag
    24601 cgccagagcc agcatacctt tcaggatccc ggttgccgcc atcaaaccga tcagaggcgt
    24661 aaaaatacct gaaataacat aaacaaagcg gtttagcaga ttacctttct catcattttc
    24721 cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg ttgaccgcca ggaagacatc
    24781 ggccacatgg ttacctatga ccacctgaaa ctgaccaccg ctttccacca ccataataat
    24841 accgggggtc tttttcagta cctcagcttg cgctttgctt tcatccttta acttaaaacg
    24901 taatcgcgtt gcgcaatgca tcagactcac aatgttatct gcgcccccga ctcctgcgac
    24961 tatttttctg gctaactccg tcataacttg ccctctaccg ctttgcggca aaactccaaa
    25021 aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat ttttttcagg ttttgcccgc
    25081 ttagtgcggt aacaatcctt tactcagtaa taatatttca gtgttctttg cgcacgcgct
    25141 ctatatttat ggctaaaaac ataatctctg cgggtgaaat tttacgttga tactgcaaac
    25201 caataaaaat ggcgatccgt tccgcacatt gccatgcttg cgggtaattt tgttttactg
    25261 cttgttgtaa tgattcatca ctatcgttaa ttgaagcatg ttcaagaata cgccaggata
    25321 aaaacttcag atgtgtaacc agtcgctgat aactcaagct ttcttcctgg taattaaggc
    25381 tgaactgaaa ttttattaat tgcagcattt cgcgcattaa ctgcgtgaca cctgcaacat
    25441 cctccatatt tccgctcatt tgggcactga ccagatgcat ggcaataaag cccacttcat
    25501 ctttcggtaa ctgcacgccc aaccgtttat caataatggt taacgcttct tcccctagct
    25561 ggaactcttt cgggtaaagc cgctggatat cccacagcaa cgggttgggc agcaacacgt
    25621 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
    25681 cctgtaattt tcccaagcgc tcctgcgcta aagagataat acgatcacag gttgccatca
    25741 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
    25801 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
    25861 caattcctcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
    25921 tattgttgag aattttggtg atttgtatgt tcatagcaag gacctttttt ataaacaaaa
    25981 aaacccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa tgcagtaaca
    26041 atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat taatttaatt
    26101 gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc gcagttatta
    26161 actttgtgta attttaggaa tttataaagt tatatataac aaatcccaat aattaagtta
    26221 ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt ttatccagct
    26281 catcaccgcc tacatgacgg aaatcctgcc ccttcacgta gtagaagata aactcacaaa
    26341 tattctggca gcggtcgcca atacgttcga tagaacgcgc gcagaacagc gcagttagta
    26401 cgctcggaat ggtacgcgaa tcttccatca tgtaggtcat cagttgacga acaatacctt
    26461 cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg tcaatgtcca
    26521 tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg cccagcgact
    26581 ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca gtacggcaga
    26641 ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg atcaccataa
    26701 ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg caggcttcat
    26761 cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc ttcgccagat
    26821 cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc accatgccgc
    26881 ccatggtcat cacctgcgtg cggatacttt ccagttcggc attgaactgg ccggaaatat
    26941 gcttattaag attgagactg tccataacgc actcctgaat caaccgtaac gaccggtgat
    27001 gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt tgctgaactc
    27061 aatcaattcg cccaggtaca taaacgccgt gtggtcggaa caacgcgcag cctgctgcat
    27121 gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca gctcttcaat
    27181 acgcccggta gagatagggt cgagcgccga acacggttcg tcgagcagca gcacttccgg
    27241 gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag agagagagta
    27301 accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg tcaatgccca
    27361 ctgcacgcgc tcgtccatgt cggcacggga gagcttctca aacagacgaa cgccaaaagc
    27421 gatgttgtcg tagatggaca tcggaaacgg cgtcggtttc tggaacacca tgcccacttt
    27481 cgcacgcagc agggcgatat cctgagagtt ggtcaggatg ttgtcgccat caagcagaat
    27541 ttcaccttcc gcacgctgct ccgggtacag ttcaaacatt ttgttgaagg tacgcagcag
    27601 cgtcgattta ccgcagccgg acggcccgat aaacgccgtt acctggtttt tagcgatatc
    27661 caggttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat tacgaacctg
    27721 aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat cgtacctgat
    27781 gcttttcgct aatcaggctt accgtaggcc gggtaaggcg ttcacgccgc atccggcaaa
    27841 gtgttaaccg tgtttattct tcgcaaaaac aacgcgcgcc agaatgttca gcagcaggac
    27901 gcacagggta atgatcaata ccccggccca ggccagttgt tgccattcgg caaacgggct
    27961 catcgcaaac ttaaagatcg tcaccggcag gttggcgatc ggctgcatca tgtccgtgct
    28021 ccagaactgg ttggagagcg cggtaaacag cagcggcgcg gtttcaccgg caatacgggc
    28081 aatcgccagc aggataccgg tcataatccc ggacaccgac gctttcagcg taatcgcgga
    28141 gatcatcttc cacttcggtg tacccagcgc ataagccgct tcacgcaggc tgtccggcac
    28201 cagtttcagc atgttctcgg tggtgcggat aacgatcggc acctgcaaca gcgccagcgc
    28261 aattacgccc gcccagccgg agaagtgctc catctgtgcc accacgatgg tgtagacaaa
    28321 cagaccaacc acaatcgacg gcgcggagag cagaatgtcg ttaatgaagc gaatcacttc
    28381 tgccagccag gatttacgac catattccgc cagataaatc cccgccataa tgcccagcgg
    28441 cgtaccgaat accgtggccc acaaaattaa cagcccgcta cccgccaggg cgttagccag
    28501 accaccacct tccgtattgg gcggcggcgt catttcagtg aacagcgcca gcgacatacc
    28561 gtcgataccg cgagtgatgg tggacattaa aatccagatc agccagaaca ggccgaaggc
    28621 catcgtcgcc atcgagagcg tcagcgcaat acggtttttg aggcggcgac gcgcctgcat
    28681 tttgcggcga gattcagcca gcgccgcagt ggtttgcatt tcaaccatag ccattagcgt
    28741 gccccctcat tcttagccag gcgcataatc ataaacttcg atgcggcgag gacgatgaag
    28801 gtaatcacaa acaggatcag gcccagttcc atcagtgcgg caacgtgcag accggattcc
    28861 gcttccgcaa attcgttcgc cagcgcagag gtgatactgt tgcccggcat atacagcgag
    28921 gcgctgtcga gctggtaggt gttaccgatg ataaaggtca ccgccatggt ttcaccgagc
    28981 gcgcggccca gccccagcat gatgccgccg ataacaccat ttttggtgaa cggaagaacg
    29041 atacgccaga taacttccca ggtggtgcag ccaataccgt aggccgactc tttcatcatc
    29101 accggggttt gttcgaacac atcacgcatt accgccgcaa tgtacggaat aatcatgatg
    29161 gcgaggatca cgcctgccgc gaggataccg ataccaaatg cggggccaga gaacagcgcg
    29221 ccaacaatcg ggatattcga catgatattg ccgaccggct cctgaaagta aacggcgaac
    29281 agcggcgcaa agataaacag gccccacatg ccgtaaacga tacttggaat ggctgccagc
    29341 agctcaatgg cgatacccag cgggcgtttc agccagccag gcgcaagctc agtcaggaac
    29401 agggcgatac cgaaactcac cgggacggcg atcagcagcg cgataaacga agtcaccaac
    29461 gtaccgtaga tcggcaccag cgccccgtag atatcgttcg gtgcatccca ctctttggtc
    29521 cataggaaag ccagaccaaa tttctgaatg ctcggccagg aggagatgat cagagagaca
    29581 ataatgccac ccaacatcaa tagcacaatc agcgccgcca gttttaccag cacgctgaaa
    29641 attatgtcgc cctttttacc cggtgggtta aaagcaggct tggttgcagc cataagttac
    29701 tcttcagtta aacgcgttta caaagtcatt ttgtaatgcc ggatgcggcg taaaacaccg
    29761 tacccggcct ggagttttat tagtacagcg gcttaccgct actgtcttta atattggtct
    29821 tccacgcagc gcgaacctgt tcaactacac tatccggcag gctggcgtaa tccaggtcgt
    29881 tcgcctgttt agccccggtt ttgtacgccc agtcgaagaa tttcagcact tctgtgcctt
    29941 gttctggttt cttctgatct ttgtggatca gaatgaacgt ggtagaggta ataggccatg
    30001 catcttcgcc tttctggttg gtcagatcct gagcgaaggt tttgctccag tctgcacctt
    30061 ttgctgcatt agcgaagttt tcttcggtcg gactaaccgg tttaccatca gcggagatca
    30121 gtttggtgta cgccaggttg ttctgcttcg cgtaagcata ttcaacataa ccaattgcac
    30181 ccggcagacg ctgaacgaac gcggcgatac cgtcgttacc tttaccgccc agaccgatcg
    30241 gccattttac ggtagagcca gtaccaacgt tgtttttcca ctcttcgttc actttcgcca
    30301 ggtagctggt gaagacgaag gaagtcccgg agccatctgc gcggcgtact acagcaatgt
    30361 tttgtgaagg cagtttcaga cccggattca gtttggcgat ggcttcatca tcccacttct
    30421 tgattttgcc caggtagatg tcgccgaggg ttttaccatc cagcaccagt tcgccagact
    30481 tcagccctgg aatgttaacc gccagcacca cgccgccaat cacggtcggg aactggaaca
    30541 gaccttcctg agccagtttt tcgtcagaca gcggcgcgtc agaggcacca aaatcaacgg
    30601 tattagcgat aatctgtttt acgccaccgg aagaaccgat accctggtag ttaactttat
    30661 taccggtttc tttctggtaa gtgtcagccc atttggcata caccggcgca gggaaggttg
    30721 cacctgcacc tgtcaggctt gcttctgcaa acacagagaa agcgctcatc gataaggtcg
    30781 cggcgacaac agttgcgacg gtggtacgca taactttcat aatgtctcct gggaggattc
    30841 ataaagcatt gtttgttggc tacgagaagc aaaataggac aaacaggtga cagttatatg
    30901 taaggaatat gacattttta tgacagaaag gaattaccaa ataacttact gattttaaat
    30961 gtgaagatga aaatcccaca tgtcatcttt ttgtcatgat gcatttaaaa gtgataaaga
    31021 aatgggtgag atgacaacga tgtgatgaac aatcgttgtc agacctatta gcgcacaatc
    31081 tcaacagtta tcgtcgctgt tgcagtaaat tgccccgggg ctgggcgagc tccagtaagg
    31141 ctcgcaggag cagctgaaaa agttacacta cctgattttt caccatacac tgttgattta
    31201 cccatgtctg ttggcaactc accaccgttc actttgattt ccttattatt tttgtcgtaa
    31261 atttttatcc ccacgttaga gtttgaagtt acgaccattg aattgtccgt attgggttcg
    31321 gtagcataaa aagacgtact gatatcatcg gtattcagta tccccaggtt ttcacactca
    31381 atagtgaccg ttttagtaat tttatgtgtt gttaccgcac ttccaacaac agatgaaaaa
    31441 tctgcagcag gtatttcttt aaaatcaaaa taaactggct gtaaattatt tatttcgcaa
    31501 ctttgcggag ctgtaatatc gccccgaata taaacatcag ccatcggctg tgagtaatcc
    31561 accggggtgt cacggctaat tgttccatac aatgtcacta tctttgtttc aggaataatc
    31621 aattttcctg caaatgtctt cttgatataa aaatatacta tcgctttagc cccactagat
    31681 atacttaaag gttcaatgcg gttgcaattg tactttgtat taccagacgg gttgttagca
    31741 tgatattcaa aaggtgcatt cacataccca accccaagaa tatctatagc aatcccaaca
    31801 tcaaggttcg gattaagatc ataataatta atattagagt gaaccccagc ataagttaat
    31861 gcctggttta tttttgacgt ataatagata taggggaatt ctcctacgtt accagtatca
    31921 caatgacagc gtccaggata tgaggatcca ttagttacag tggtgttaaa tgtcgttcca
    31981 accttatttt cgttcgcggt aataagatat cccgagaaat caagcgtact gtgaaatacg
    32041 ccattatcag gggtacagat acctttagga gctgcaagtg ctacactact taacgagagc
    32101 ataaacacag caaagcacca ctgtttgata tacttgttca taaaaatcct tttaatgaca
    32161 tgtcacaccc tgttcaatta atccgctctc actttctggt gtcgaaagtg aataattgaa
    32221 gatgcatttt gttgaggcgt tttctcccca ggataacgta acctgtcctt tttctggtag
    32281 accagaaaga taaagttcgc cacggtcacc gacaattcct gaatctgcca aacttgcatc
    32341 ttgtatcgca gcaatggctc caaaaggtac cggtttacca tttgcattta acactctgaa
    32401 gagaacgcga taacccacat gagtcacaaa ctccgcttta accactgcac cacgcgtggg
    32461 aaccatcgta acgacggtat tatcaagttc tatattcgac ttcgagaaat agttatcgct
    32521 gattgctacc cgattttcat cataagggtt tagctgtgtc ttgaccgtat agccgcgcca
    32581 gtccgtcgca gcaccgcgca tattatctat ttctaacccg gcggccccag gtgctttaac
    32641 aagagcaata gtttcaccta actcctgact taacgttacc ccttcgctgt gtgccagaat
    32701 tccccctctg ataccataat tcagcctctg ggaattttgg ctgtagttat agctgccgtt
    32761 caccgtgcca taagagccat cataggtcag ggacatatta ccgccaacct catcatcatg
    32821 actgttatag gactgcatta aattccatga aaggttattt ctttccagcg ccgtaccgct
    32881 gagtccgagt tgatgtgagc taccggaatt atcgttttgc gaaacagaat aggtggcgta
    32941 actattaccc caccagcccg ataaaggaac agataaagag agcatgtaga tctgctcact
    33001 ggtgttatca ctggcatggt tgttccccgt cccctgtctg gcagtattgg aataactcca
    33061 ggagacgttc cacgagagac gcttccagct actgctgtag ccaaattgca ttaatcgttc
    33121 gacatcatcg gtatgccagt attgttgccg gcttaatgtc aggtataaat tgccataatc
    33181 tctccccaac gactgcgtta gcgttccctc cacgcgacta cgtcggttac ctgtttccca
    33241 aaagtcttca gggctgttgc gccgcgatgc ccactcattg agggtatagt agccctcggt
    33301 ggagtaacga tagccaacca ggcgcaagct agtgtctgtg tcgtcaaatc gttttgaata
    33361 gagaaagcga tatgactgac ccgtttctgt atcatcatgg ctaaaattcg cacgagcatg
    33421 tgtcacatcg aaagaaattg cgcccaaagc gccaagatct ttaccgacac ccagagctgc
    33481 agaaccataa ttttcagcag cctgcatacc tccatataaa gtgatcccgt gggaaaaacc
    33541 atgaagtatt tgcgcctgaa ggacgtccgg tgtaaatccg tcttcatcgc gcaattcacc
    33601 cacgctaaca tcaacatctg tctgaccttc acgtttaaga atcgccaatg aagcataagg
    33661 ttgggtgaat cgttgttcgc taccgtcttc ttctttgatg gttacgtcca aatcgccacc
    33721 tgtactggcg gtgttgagat cggtgatctc aaaagcacct tgtggaacgg cagactgata
    33781 aatcacgtag ccattctgcc gaatgatgac ggtggcgttg gactttgcaa tcccacgaac
    33841 cacaggagca aagccattct ggctggcggg tagcatatcg ttatcagtat acaaacgcgc
    33901 gccgcgaatt tgcgtactgt cgaaaatatc gctcgccgtc catgtatcac caatcataat
    33961 ctggcttctt aacgcggcaa tatcgcgctg tatccaggta ctaatggagt tgtattccgc
    34021 acggccatcg ctggtgttta aagtactgta attacgtagc cgccacggtc caatattcag
    34081 tccattgcgc agattgagat aactggtatc tgtctcttgt gaaccataat ctgaacgtag
    34141 tgtatgtgaa ccagaaaaat cgtagttgat ggtgagcgcg ttaatcccct catcccaacg
    34201 cgtggggctg atgtaatcat aagccatcgc gtccatgtag atttggggaa tggatatatt
    34261 cagctgcaat ttatcactgt caaactgcca ggatgagcct tcaatacgtg gagcaggatc
    34321 gaggcaaacc gtctcatcca catcattgaa cgatttgagc gcatcaactt ttatgccaaa
    34381 ggaggacagt gtttttttaa tggcaataaa gcaagcgtgc agatcagcat ctggattatc
    34441 ctgaacaaat gtcacatcct gacgttcaaa cattttaccg ttgataaaaa tatcaacgct
    34501 ataaacacct gggggcatca ggtttgcccg attaaactgg cgaatatcga ctccggattg
    34561 atggtcgatt tccagaagcg ccggattgaa tacactgacc tcggccatca ggttttgcga
    34621 gaaaatcaga cacgtccctg ccgtgaggcc aaccactatt ctggtcgtca tcataatacg
    34681 cctatcctta tcccttgctt acagcgtcat ttttatcggg tcactgactg cgccgtaatc
    34741 attaataaat cgccaggtca tttggcctcc cgcagaatta gcaggtaaag gtaacgtgag
    34801 cgtttcaaaa ggacggatca tattgagggg cacatcatgg ccatcaacga tgacatccat
    34861 cacactgata tgaattgggt tgggattctc tgctatcaga tttcgccctt cccggcgcca
    34921 tttcactttc ggtgcagcat cttcgggaat aaggcggata tttgctggtc gccagaacag
    34981 cttgatacgc gtttttactg ctatttccag cgagttcgtc gctttgttgc tcgctgatgg
    35041 cggaatattt ttaatattca gccagaagac tgactcgcga tcatcaggca atgacacacc
    35101 tttggtgtga ataatgcgta atattttttc ttgcgaaggt tctaatcgtg atactggcgg
    35161 aataacggta aacggcgctt tgcttttatc ctctttatta aatggatcta cccaagcctg
    35221 gatgagataa ggtatttttt cttctttgct aaatacagaa atagatttat cagcattatt
    35281 acttaagtaa accacccgcg taccaccaat gacaacgcct gcataagtta atgctggttg
    35341 gcctaaaaaa acgctgagta taatcgcggt aaataactta catgaaactg ttgttttaaa
    35401 gagcctgatt aaacaacttt ttctggacat aacgactccc tggccaaagc caactgaata
    35461 aaaaggccct tgataaatta tttcttatat tcgacagtaa attgtgaatc agcgttcgcc
    35521 gtaccagccg ttacggtcgc attggttgcc acatagcgtg caataaactt catttcagcc
    35581 ttgccttcag catcgatagc ggtataagca gagtcgttat aaagtttaac ctgcgaatta
    35641 tcggactggt taaaaatacg aataccaacg ccggttgccg caatcgcttg tcctgtaccg
    35701 gtaaaatcac cagggttgcc tgctgtaagc ggcgtaccca ctttcaggtc gccgtcgccg
    35761 ccaggcgctt ctgtaccatc aaaacgaacg gctgcttgag tgtacgttgc cgggcaattt
    35821 tcaagcttaa tactgaactt ctgtggcgac gccagactac ctacacctga aaatgcattt
    35881 ttactgactt tacccagtgg gacaatttga tcggctgtat cggaggtaac ttcacaagta
    35941 gtgtcgacaa tttcaccgac gaagtgaaca acaccatctt ctgccagtgc atgcccggcc
    36001 agcattagag atgccagagt gaatgcaccg cctataatat tacgcttcat aaaaactcca
    36061 ttgatataat atatccttta ccatgaatca tcacttatat taataagtgt atttttgatt
    36121 caaaggaata gtacaccaaa gagacggctt tatctaaaaa taatattaat tacgcaaaaa
    36181 taaaatataa atttattgtt taaatattat ttaaacagta aaaaatcaag tttgttatca
    36241 gacattaatg ataattcaga atatatttat atagaaagat agatgaattt attgatgtca
    36301 gttataaaaa agggccaccg gtcggcagcc cttttttatc tatgatagtg attactcaac
    36361 cgtaaccgat tttgccaggt tacgcggctg gtcaacgtcg gtgcctttga tcagcgcgac
    36421 atggtaagcc agcagctgca gcggaacggt gtagaagatc ggtgcaatca cctcttccac
    36481 atgcggcatc tcgatgatgt gcatgttatc gctacttaca aaacccgcat cctgatcggc
    36541 gaagacatac aactgaccgc cacgcgcgcg aacttcttca atgttggatt tcagtttttc
    36601 caacagttcg ttgttcggtg caacgacgat aaccggcata tccgcgtcaa tcagcgccag
    36661 cgggccgtgt ttcagctcgc ctgcagcgta ggcttcagcg tgaatgtaag agatctcttt
    36721 cagcttcaat gcgccttcca gcgcgattgg gtactgatcg ccacggccca ggaacagcgc
    36781 gtgatgtttg tcagagaaat cttctgccag agcttcaatg cgtttgtcct gagacagcat
    36841 ctgctcaata cggctcggca gcgcctgcag accatgcaca atgtcatgtt caatggaggc
    36901 atccagacct ttcaggcgag acagcttcgc caccagcatc aacagcacag ttaactgagt
    36961 ggtgaatgct ttagtggatg ccacgccgat ttccgtaccc gcgttggtca tcagcgccag
    37021 atcggattcg cgcaccagag aagaacccgg aacgttacag attgccagcg aaccaaggta
    37081 acccagctct ttcgacagac gcaggcccgc cagggtatcc gcggtttcgc cagactgtga
    37141 caaggtgatc atcaggctgt tacgacgcac ggcagatttg cgatagcgga attcagaggc
    37201 gatttcgacg tcgcacggaa tacctgccag cgattcaaac cagtaacggg aaaccatacc
    37261 ggagttataa gaagtaccac aggcgaggat ctgaatatgc tcaaccttcg acagcagttc
    37321 gtcggcgttc ggtcccagct cgcttaaatc aacctgaccg tggctgatgc gcccggtaag
    37381 ggtgtttttg atcgcgttcg gctgttcgta gatctctttc tgcatgtagt gacggtaaat
    37441 acctttatcg cccgcgtcat attgcagatt ggattcgata tcctgacgtt ttacttccgc
    37501 gccagtttta tcgaagatgt ttaccgaacg gcgagtaatt tccgcaatat cgccctcttc
    37561 aaggaagata aagcgacggg tcaccggcaa cagcgccagc tggtcagaag cgataaagtt
    37621 ttcgcccata cccaggccaa tcaccagcgg actaccagaa cgtgccgcca gcagggtatc
    37681 cgggtgacgg gagtccatga tcactgtacc gtacgcacca cgcagttgcg ggatagcacg
    37741 cagaacagcc tcacgcagag taccgccctg tttcagctcc cagttaacca gatgggcaat
    37801 tacttcggtg tctgtttcag aaacgaaggt atagccacgc gcttttaact cttcgcgcag
    37861 cggttcatgg ttttcgatga tgccgttatg caccaccaca atgtgttcag aaacatgcgg
    37921 atgcgcattc acttctgaag gttcaccgtg ggtcgcccag cgagtatgag caatacccgt
    37981 accgccatgc agaggatgtt cttccgctgc ctgagccagc atctggactt taccgaggcg
    38041 acgcaggcgg gtcatatgac cttccgcatc aacaacggcc agaccggcag agtcatatcc
    38101 gcggtattcc agacgacgta aaccttcaag aaggatttct gctacatcac gttgcgcgat
    38161 cgcgccaaca attccacaca tagtttttga ttccgattta tatcgttgtc ggtcaacctg
    38221 tatgcccgtt ttttcgggcg ccccgagcct tgtagagagt ggggttattt ttatagttac
    38281 tgcttgtggg agggagatta tgttatctcc tcatcccatg tgaccggatt acccggccag
    38341 aatcactttt tctttaccgg acgacgccag ccttctttct gagtctgcgg cacacggctg
    38401 atagctaatg cattttcgcc gacattacgc gtcacagttg tacccgcagc aatggtcgcg
    38461 cctttgccta ctgttaccgg ggccaccagc tgagtgtcgg aaccgacaaa cacatcgtcg
    38521 ccgataatgg tcttaaattt attcgcacca tcgtagttgc aggtaatggt tcccgcgccg
    38581 atgttaacgt tatcgccaat ttccgcatcg cccaggtaag tcagatgacc agctttcgag
    38641 cctttaccca gacgcgcttt tttcatctca acaaagttac cgacgtgtgc accttccagc
    38701 aactcagcac caggacgcag tcgggcaaac gggccaatag tacaggccgc tgccagattc
    38761 gcgtcttcca caacggtata cggactgatt tcgcaatcat cgccaatcac gctgttttta
    38821 atcacgcaac cggtgccgat tttcacgcga tgaccgagag tcacgttgcc ctcgatgata
    38881 acgttagtat caatttcaac atcgcgcccg tgagtaagcg taccgcgcag atcaaaacgc
    38941 gccggatcgc gcagcataac gcctgctaac agcagttttt cagcctgttc ggactggtaa
    39001 acgcgctcca gacgggagag ttgcaggcgg ttattcacac cttctacttc gcttaaacgt
    39061 tgcggatgaa cggcgacgat ttcacgccct tcctgatacg ccagcgcaat aatgtcggtg
    39121 atgtagtatt caccctgagc gttattgttg gtcagcttcg ccagccagcg tttcatatct
    39181 gcgccgttag cgatcagaat gccggtgttg atctcctgaa tctgacgcag ctcgtcggtg
    39241 gcatctttgt gctcaacaat gccggtaact ttgccatttt cacgggtgat acgtccataa
    39301 ccggtcggat catccagttt caccgtcagc agaccaatgc caccctgcgg tttagcatca
    39361 cgcaggcgct ggagtgtttc gacagagatc agcggcacgt cgccgtagag cattaaaatg
    39421 tcttcatcat cggcaaagaa aggtgcggcc tgctgcattg catgacccgt acccagctgc
    39481 tctgcctgaa gcacccagtt aaggttgtcg tctttcagcg cctgtttaag cagatcgccg
    39541 ccgtgaccgt acaccaggtg aacgtgcgct gcgcctaatt cattcgcagc atcaatgaca
    39601 tgctgaacca tcgctttccc ggcaagggta tgcagcactt tcggaagatc ggaatacatg
    39661 cgcgtgcctt tgcctgcggc aaggatcact acgctcatag cattattcaa catacgcgtc
    39721 ctgactgtaa tttgagaacg aatttaaacc gcttcacctt gaaaaaacta catttttttc
    39781 atcgtgaaat ggacagagga taaattgttc aataacggat tatccccgct gacgaaagtg
    39841 ccattttcga ccatcgtcac gccttttgtc tgcagaaaat taaggcagta ccttcattta
    39901 agcgtcaggt ggatgttttt gctcttattt cgatcaatga ataaacggga aaacaggggt
    39961 gttttatatc agcaggatct atgtgaacgc tcttcaggac ggatcacgcg taaaaaaaag
    40021 ccagcctgtt tccagactgg cttttgtgct tttcaagccg gtgttacatc gcttttttgg
    40081 tcaactcgat aacgcgcagc tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt
    40141 aatctacgtc gccgtgagag ctgctaatgt gctcttcagc cttacgtttc gcttccatgg
    40201 ctcgcgcttc gtcgagatcc tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc
    40261 caggctgcac ttcaagaatg ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt
    40321 tcacgatgcg aatcatacca ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga
    40381 tccccagttc accttcgcta cccgttacct ggattttctc gaccagacca gagaacattt
    40441 gttgctctgc gctgacgacg tccaggtggt aagtcattgc catatcaccc tccgattaag
    40501 gcgttaaagt tttttggctt tttccacagc ttcttcgatg gaaccgacca tgtagaacgc
    40561 ctgctccggc aggtgatcgt attcgccttc catgatgcct ttaaagccac ggatggtgtc
    40621 tttcagggag acgtatttac ccggagaacc ggtgaatact tctgccacga agaacggctg
    40681 ggacaggaag cgctggatct tacgagcacg cgctaccacc agtttgtctt cttcagacag
    40741 ttcatccata cccaggatgg cgatgatgtc tttcagttcc tgataacgtt gcaggatgga
    40801 ctgaacgcca cgcgcggtgt cgtagtgttc ctgaccaacc accagcgggt ccagctgacg
    40861 gctggtggag tccagcgggt caacggccgg gtagataccc agagacgcga tctgacggct
    40921 cagtaccacg gttgcgtcaa ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc
    40981 atccgcaggt acgtataccg cctgtacgga ggtgatagaa ccagttttgg tggaggtgat
    41041 acgttcctgc agaacgccca tctcttccgc cagggtcggc tgataaccta ccgctgaagg
    41101 catacggccc agcagtgcgg atacttccgt accggccagg gtgtaacgat agatgttgtc
    41161 aacgaacagc agaacgtcac gaccttcgtc acggaatttc tcagccatgg tcagaccggt
    41221 cagagcaacg cgcagacggt ttcccggcgg ctcgttcatc tggccataca ccagggatac
    41281 tttgtcgata acgttggagt cggtcatttc gtggtagaag tcgttaccct cacgagtacg
    41341 ttcacctacg cccgcaaaca cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag
    41401 ctccatcatg tttacggttt tacctacacc cgcaccaccg aacagaccaa ctttaccgcc
    41461 cttagcgaac ggacacatca ggtcgataac tttgataccg gtttccagca gttcctgaga
    41521 gtttgacagc tcttcgtagg aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc
    41581 gatctcgcct ttcatgtcga ccggttcacc cagtacgttc atgatacggc ccagagtcgc
    41641 tttacctacc gggacttcga tcgggtgttc gaggtctttt acatccagac cgcgacgcag
    41701 accgtcggag gaacccattg cgatggtacg cacgataccg ccgccgagct gctgctgaac
    41761 ttccagcacc agacgctcat taccattttg cacctcaaga gcatcgtaca cgcgcggtac
    41821 ggcatcctga gggaattcga cgtcaactac ggcgccgatt acctggacaa tctttccagt
    41881 agccatctta aatcctctac gaaataacct gtttaaaccg cggcggcccc cgagacgatc
    41941 tcggtgagtt cctgagtaat gctggcctga cgagctttgt tgtataccaa ctgcagctct
    42001 ttaatcaggc tgccgccatt gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc
    42061 tcgctggcca ggttttcaac cacgccctga taaacctgag attcgacata acgacgcagc
    42121 agggtatcca gcaacgcctt cggatcgggt tcgtacaggt aatcccagga tttatgtttc
    42181 agatcatcat catctgatgc cggtaacggc agcagctggc tgatggtcgg aacctgagac
    42241 atggtgttaa taaatttgtt gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag
    42301 gcctgcaaca tcacttttac cggaccgatc agttcggaca gggaagggtt atcccccatg
    42361 ccggtgacct gggcaacaac attgccgccc acggagttga agaacgacac gcctttcgag
    42421 ccgatcattg cgaggtcgca ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc
    42481 agttttttga acaggttaat gttcaaacca ccgcacaaac cacggtcggt cgacaccacc
    42541 aggtagccca cgcgtttaac gtcgcggtct tccaggtaag ggtgcttata ttccagatta
    42601 ccgtgtgcaa ggtgaccaat cactttgcgc atggtttctg cataaggacg gctggccgcc
    42661 atgcgatcct gcgatttacg cattttggaa gcggcgacca tctccatcgc tttagtgatc
    42721 ttttgcgtgt tctggacgct tgcgatctta ctacgtatct cttttgcgcc ggccatgagc
    42781 ttctcctcaa tgccttgcgg cctgccctaa ggcaagccgc cagacgttac caggattggg
    42841 ttgctttgaa ggaatcgagg atgcctttca gcttgccttc gatttcgtcg ttgtagccac
    42901 cggtctggtt gatctcttgc atcaacggag cgtgatcacg gtcgacgtaa gccagcagag
    42961 cggcttcgaa gctgccaatt ttcgacagtt caacatccgc caggtaacca cgttctgctg
    43021 cgaacagaac cagagactgc tgcgcaacgg acatcggcgc atactgtttc tgtttcagca
    43081 gttcggtcac tttctgaccg tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg
    43141 caaactgaga gaacgctgcc agttcacgat actgtgccag agcggtacgg ataccaccgg
    43201 acagtttttt catgatcttg gtctgtgctg caccaccaac acgggatacg gaaatacccg
    43261 ggttaaccgc aggacgaata ccggcgttga acaggttggt ttccaggaag atctgaccat
    43321 cggtaatgga gattacgttg gtcggaacga acgcagaaac gtcacccgcc tgagtttcga
    43381 taatcggcag tgcggtcaga gaaccggttt tccctttcac ttcacctttg gtgaaggctt
    43441 caacgtattc ggcgttaaca cgtgcagcac gctccagcag acgagagtgg aggtagaaaa
    43501 cgtcgcccgg gaatgcttca cgtcctggcg gacgacggag cagcagggag atctgacggt
    43561 aagcaacagc ctgtttagac aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac
    43621 ggaagtattc gcccattgcg caaccggcat acggtgccag gtattgcagt gcagcggatt
    43681 cagacgcggt tgctaccaca acgatggtgt tagccagtgc gccgtgctct tccagtttac
    43741 gtaccacgtt agaaatggtg gacgctttct ggccgatagc gacatagata catttgatac
    43801 cggaatcgcg ctggttgatg atggcatcga tagccagtgc ggttttacct gtctgacggt
    43861 caccgatgat caattcacgc tgaccacgac cgattgggat catggagtca acggctttat
    43921 aaccggtctg taccggctga tctacggact gacgttcgat aacgcccgga gcgattgctt
    43981 ctacagcaga gaagccgtcg tgatccagcg gacctttacc gtcgattggt gcacccagag
    44041 tgttaaccac acggcccagc aggccacggc caaccggaac ttccaggata cggccagtac
    44101 acttaacttt catgccttcg gcaaggtcag cgtacggacc cataacaacc gcacctacag
    44161 agtcgcgctc gaggttcagt gcgatagcgt aacggttacc cggcagggag atcatttcac
    44221 cctgcataca atcggccagg ccgtgaatgc ggataacacc gtcacttaca gaaacaatag
    44281 taccttcgtt gtgagcttca ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt
    44341 cgctgatttc ggtggaattc agttgcatgc tccagtcccc ttaagactgc aagacgtctg
    44401 caaggcgctc aagacgaccg cgtacgctgc catcaatgac catatcaccc gctcggatga
    44461 taacgcctgc cattacagac ttatcgattt tgcaattcag cttaactttg cgtgacagac
    44521 gtttttccat cgcagcagaa attttcgcga gctgttgttc actcagtgcg gcagcggaaa
    44581 tgacgtctac ctcagcggta gcctcactca cggcacgaag gtgaataaac tgctccagaa
    44641 catccgggag cgcgttaaga cgaccatttt cagccattac ccgaatcagg ttctgaccgt
    44701 tttcgtccag ttgctcacca caaactgcga taaacgactc ggcgagcgtt tctggcgcaa
    44761 gcgcgccaga gagaagctct gccatttgtt cgtttttggt tacctcggcg gcaaacgcca
    44821 gcatgtcctg ccagcgttct acactttggt gttcgacggc aaagtcaaaa gctgctttgg
    44881 cgtaggggcg agctaccgta ataaattcag acatcagccc ctccctcctt acagttcagc
    44941 gacaagttta tccacgatgt cgctgttagc agcttcatcc acggaacgtt cgatgatctt
    45001 ctcggcgcca gcaacagcca ggatagcaac ttgcttacgc agctcttcac gggcacgttt
    45061 acgctcggct tcaatttctg cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc
    45121 ctcagctttc gcttcgtcca gaatctgcga gcggcgtttg ttcgcctgct cgatgattac
    45181 ctgggcttcc gctttcgctt ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag
    45241 gtccttatgt gctcgttctg cggaagcaag gccgtcagca atttcttttt gacgtttttc
    45301 gatggctgcc attaatggcg gccatacgta cttcatgcag aacagaacga acaggacaaa
    45361 cgcgatggcc tggccgagga ttgttgcgtt aagattcaca gcacaatgcc tctatttagt
    45421 taacgttctg atattgctct ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca
    45481 cgtacagacc cagacctaca gcgatcatcg ggatagcatc caccagaccc ataacgataa
    45541 agaactgagt acgcagcaga ggaatcagat caggttgacg cgctgcgcct tccaggaatt
    45601 tacccccgag gatgccgata ccgatcgcag caccgattgc cgccagaccc atcatcacag
    45661 cggcagccat gtacagcaga tccatattca ggttttccat gacagtctcc agtttgtttc
    45721 agttaaaacg tagtagtgtt ggtaaattaa tgttcttcag acgccatcga cagatagacg
    45781 atcgtcagaa ccatgaagat gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc
    45841 cacggcacat tcaggatcca ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa
    45901 atcagctcac cggcatacat gttaccgaac agtcgcaaac caagtgaaac tggtttggac
    45961 agcaggctta ccccttcaag gattaagttg acaggaatga acgcccagtg attgaacggc
    46021 tgcagcgtca actctttcgt gaagccgccg atgcctttca ttttgatgct gtagaacaga
    46081 atcaggataa atacgcccaa tgccatagac agcgttacgt tcacgtccgc agacggaacc
    46141 acacgcagtg caggcagacc cagtacatgt tcagcaatgt acggcagcag gtcgataggc
    46201 agtaaatcca tcaggttcat caggaatacc cagacgaaga tcgtcagggc cagcggagca
    46261 atcagcttgc ttttgccatg gtacatgtct ttcacgctac cattaacaaa gccgatcacc
    46321 agctcaatcg cggtctgaaa cttacctggc acaccgctgg tcgccttttt ggctacgcta
    46381 cggaataaaa ccaggaacaa cagacccagc accaccgaga agaacatgga gtcaatattg
    46441 attgtccaga aggtggctgg ggggttttgt ggatccacca gcgagaatgt acgcaggtcc
    46501 agctgaaggt tattcaggtg gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc
    46561 atgatgcctt ttaccctttg ttgttaatta cagccggtgc cagtatctga accaccagca
    46621 ccaaaaccca cgtaacgatc agcggcaaga ataccgcctt taaaaccgcc aacgccacca
    46681 ccagtaacac caacatcgcc agaactttga aagcttcgcc aaatgcgaat gtccaggcca
    46741 cccggccttt cgctggtgta tgcgcctggt gacgccaggc aaatatcata aacaaaacgt
    46801 taggcagaaa gactgccagg cccccgctta ttgcagagac gccccagaag gggtctttga
    46861 ggctgaacag caatccactt gctatcacca ccagtaactg aacgagcaga agcttccgag
    46921 caacgtttcg actcacgagc gacacagaca tcacgttttt cactcctgct cccttcgagg
    46981 tatgccgcgt gtcgtataaa actttcttta aggcttagag tcaagcatca aaaagcggtc
    47041 aaattatacg gtgcgccccc gtgatttcaa acaataagta gccaaaaggt gaataaatgt
    47101 ttaaatattt ttccagtgca tacaattgcg acttttctgc taaccctgtt cgatcatgaa
    47161 aaactgtaaa taacgcgtaa acactggtga taaagcgtgc ttcagatcac atattgcgca
    47221 tgttcgcgca cagcatattt atttacttgg caaatgatgc ctttgcaagt ttatgatatt
    47281 tcagtctaaa aacagatact gttttaataa atgacattta cacaacaaaa accacccatt
    47341 gacattttta ataatgtttt aacagccaat gatggttctt agcgccgatt tttagcagac
    47401 tgatattttc actaatgact tattttctgc ttaccaaaaa aagccacgtt atcttgttga
    47461 tgcaaaagag tgaacgtggc gttaaatgta accagttata tcagtagaaa acctggttgt
    47521 tgttaacagt ctaaccggtc aattttttat gatttttttg ataaaaatta aattttattt
    47581 gctttaatca ccaccagatg acgttcgcca tccagggctg gaacctgaag tttaaccact
    47641 gattcgacct gatattcttc gggcaacaaa gcgatttcat cttccggcat ttgccctttc
    47701 agcgcgtaga aacggccttg ctcaccagga agatggtggc accagctcac catatcgttc
    47761 agagaggcaa aagcgcggct aattacgcca tcaaatggcg gctctgaagg aaactcttct
    47821 accctgctct gtactggttc aatattctcc agtttaagct catgttgcac ctgacgaagg
    47881 aaacgcacgc gtttaccaag gctatccaac agagtgaaat gggcttcagg acgcacgata
    47941 gagagtggaa tgccaggcag tccaggaccg gtgccgacat cgataaaccg ttcaccttgc
    48001 agatacggtg ccaccacaat gctatcgaga atatggcgta ccagcatctc attaggatcg
    48061 cggaccgaag tcaggttgta cgctttgttc catttatgca gcatattcac gtaggcaata
    48121 agctggtttt tctggtgatc ggtaagcgaa atacctgcgt ctttcagcag taaggagagt
    48181 ttgttgagca cggtgattac ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag
    48241 gcatttttaa tgcgttatgc gctacgacgc agcataccct gttttttcag ccacaccagc
    48301 agaatggaga tggccgcagg cgtgacgcca gaaatacgcg aagcctggcc gatagaggct
    48361 ggtttgtgat cgttaagttt ggcgatcact tcgttagaaa gaccggatac ctggcggtaa
    48421 tccagtgtcg cgggtagcag ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc
    48481 tggcgcgcga tataaccttc gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg
    48541 tctgtcaatg caggggcaaa cggcgtcagc gtggttaatt tttcataagt catttccgga
    48601 cgacgcagca gatcttcacc actggcttca cgggaaagcg gcgcagtcag gtgagcattc
    48661 acttcggctg cagcttccgc cgacggggtt acccaggtcg atttcagacg ctgacgctca
    48721 cgctcgatat tctcaagttt ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc
    48781 agttcacgac cgatttcagt caaacgcaga tccgcattat cttcgcgtag catcagacga
    48841 tattctgcgc gcgaagtaaa catacgatac ggttctttgg ttcctaaagt gcacaggtca
    48901 tcaaccagta cgccgagata cgcctgagaa cgcgccggag cccaaccttc tttgtcagca
    48961 gacagacggg cagcgttaag accggccagc aaaccttgcg cagcggcttc ttcgtaaccg
    49021 gtagtgccgt taatctgacc agcaaagaac agcccctgga taaacttgct ctccagcgtc
    49081 ggtttcaggt cgcgtggatc gaagaagtca tactcaatgg cataacccgg acgcacgatc
    49141 ttcgcgtttt ccatcccctg catggagcgg acgatttgca tctgcacatc gaacggcagg
    49201 ctggtggaga taccgttcgg ataaatttcg ttagaagtca gcccttccgg ttcaaggaag
    49261 atctgatgct gatttctgtc ggcgaagcgc atgactttgt cttcgatcga cgggcagtag
    49321 cgtgggccga caccttcgat cacccctgcg tacattgggc tacgatcgag gttactgcgg
    49381 atcacatcat gggttttctc attggtatga gtgatataac acggcacctg ctggggatgc
    49441 tgggacgcat tgcccataaa cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc
    49501 agtacgctaa agtcgatggt tcgagcatca atacgcggtg gtgtcccggt tttcagacga
    49561 ccaacgcgca gcggcagttc acgcaaacgg cgagaaagcg gaatggacgg cggatcacca
    49621 gcacggccac cgctgtaatt atccagaccg atatgaattt taccgtcgag gaacgtccca
    49681 acggtgagca cgacggcttt ggcacggaac ttcagcccca tttgggtaac agcaccgacc
    49741 acgcgatcgt tttcgacaat aagatcttca accgcctgct ggaagatcat caggttcggt
    49801 tggttctcca gcgccgtacg taccgcctga cggtagagca cacgatccgc ctgagctcgg
    49861 gtagcgcgaa ctgccggtcc cttgcttgcg tttagtatcc taaactggat acccgcctga
    49921 tcgatcgctt tcgccatcag accgccgagt gcatccactt cttttaccag atgtcccttc
    49981 ccaataccgc cgatcgccgg gttgcagctc atctgcccca gagtgtcgat attgtgtgtc
    50041 aaaagcagag tctgttgacc catacgcgcc gcggccatcg cggcctcggt gcctgcatga
    50101 cccccgccaa tgatgatgac gtcaaaagga tccggataaa acatggtgat tgcctcgcat
    50161 aacgcggtat gaaaatggat tgaagcccgg gccgtggatt ctactcaact ttgtcggctt
    50221 gagaaagacc tgggatcctg ggtattaaaa agaagatcta tttatttaga gatctgttct
    50281 attgtgatct cttattagga tcgcactgcc ctgtggataa caaggatccg gcttttaaga
    50341 tcaacaacct ggaaaggatc attaactgtg aatgatcggt gatcctggac cgtataagct
    50401 gggatcagaa tgaggggtta tacacaactc aaaaactgaa caaccgttgt tctttggata
    50461 actaccggtt gatccaagct tcctgacaga gttatccaca gtagatcgca cgatctgtat
    50521 acttatttga gtaaattaat ccacgatccc agccattctt ctgccggatc ttccggaatg
    50581 tcgtgatcaa gaatgttgat cttcagtgtt tcgcccgtct gttttgcacc ggaatttttg
    50641 agttcagcct cgagtttatc aatagcccca caaaaggtgt catattcacg actaccaata
    50701 ccgattgcgc caaagcggac cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag
    50761 aaaggagaaa ggttgtccgg aatatctccg gcaccgtggg tggagctgat aatcagccag
    50821 atccccgagg cagataaatc ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc
    50881 gcctcttcca gcttttcagc caggtgttct gctacatatt cggcaccgcc gagggtgctg
    50941 ccgctgataa gagtgatatc tgccataaac cgccaccttt attaagagtg gcgtattgta
    51001 cgctgtgaac gcgttgggat ctacctgtgg aaaagtatgg gattaaaaaa gccgatcagg
    51061 gcttgatggt acgcatgatc gggttttgca ggacgatcaa tgtctcggtg gactgaattt
    51121 catcaattgt ttggatcttg ttgataagta catgctggag agcgtcgatc gaacggcaca
    51181 tcacttttat aaagatgctg tagtggccgg ttgtgtaata ggcttcagtg acttcatcaa
    51241 ggctttccag ctttgccagc gcggaagggt agtctttggc gctctttaat ataatgccga
    51301 taaagcagcc tacgtcataa ccgagctgct tcgggctgac atcaatacgc gccccggtaa
    51361 tgatccccgc ctgcttcatt ttctctactc gaacgtgaat cgtccccgga ctgacgccaa
    51421 attgtttcgc cagttcggcg taagcggtgc gcgcattgcc cattaatgct tccaggatgc
    51481 cacggtccag attgtcgatc agataatttt ccataggatt ttcttatgca gattgatgat
    51541 tcattctatt ttagccttct tttttaatga atcaaaagtg tgttaggctt tttattgaat
    51601 gattattgca tgtgtgtcgg tttttgttgc ttaatcataa gcaacaggac gcaggagtat
    51661 aaaaaatgaa aaccgcttac attgccaaac aacgccaaat tagcttcgtg aaatctcact
    51721 tttctcgcca tctggaagaa cgtctggggc tgatcgaagt ccaggcgccg attcttagcc
    51781 gtgtggggga tggcacgcag gataacttgt cgggctgtga aaaagcggtg caggtaaaag
    51841 tgaaagctct gcctgatgcc cagttcgaag tggttcattc actggcgaag tggaaacgtc
    51901 agaccttagg gcaacacgac ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc
    51961 ttcgccccga tgaagagcgt ctttctccgt tgcactcggt ctatgttgac cagtgggact
    52021 gggaacgcgt aatgggcgac ggtgagcgtc aattctcgac tctgaaaagc acggtagagg
    52081 cgatctgggc gggaattaaa gcaaccgaag ctgcagttag cgaagagttt ggtttggcac
    52141 cgttcctgcc ggatcagatc cacttcgtac acagccagga gttactgtct cgttatccgg
    52201 atctcgatgc caaagggcgt gagcgggcga ttgcgaaaga tcttggcgcg gtattcctcg
    52261 tcgggattgg cggcaagctg agtgatggtc atcgtcacga cgtgcgcgca ccggattatg
    52321 atgactggag caccccgtca gagctgggcc atgcgggtct gaacggcgat attctggtat
    52381 ggaaccctgt actggaagat gcgtttgagc tttcttccat ggggatccgc gtggatgccg
    52441 acacgttgaa gcatcagttg gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc
    52501 atcaggcgct gctgcgcggt gaaatgccgc agaccatcgg cggcggtatc ggccagtctc
    52561 gtttgaccat gctgctgctg caactgccgc atatcggcca ggttcagtgt ggtgtatggc
    52621 cagctgctgt tcgcgagaac gtcccttctc tgctgtaata atttatcgcc gccagcgtct
    52681 gagcaggcgg cttcgcatcc cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat
    52741 gccgggtttg ccgtgtgccg acatcgccac ggcatgaaag cgatgctgat gtacccgctg
    52801 cagctctttc actttactcg tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat
    52861 caccaccgcg tcggcatcaa accattccct gctttgcaag cgttccataa tggcgcgaaa
    52921 acaactggca agatcggtgc cgccacgaaa ctgctggctt aaaaaacgga ttgcttgttc
    52981 gatgccttgt gggcctgaaa gctcataacg gacgatctcg gtggaaaata gcataatata
    53041 gcagcgccgg ttttcagcga gagcaatgcg catcaaggcc aggcagaacg ctttcgcaca
    53101 ctgttcatta aagccgccca ttgagccgga agtatccaca cagacaataa acggcccgcg
    53161 cggctgttca tcgtaatctt tatgtaccac cggacgttcg atcacttttt cacgccacga
    53221 ctcaccgtgc aggcgatagg tgagcaactg tttttccacc agccgacggt aaaactcata
    53281 ctccagttcc gttatcccta gtgtcgccag ttctggcggc aggagacgta aaatatcatc
    53341 gctttgttgc agaccatcaa cctgctcagg aacagttgcc ggttcgcgca ccatggtgcg
    53401 gaaggcttcc atctgcgcat cgttacgcgg tattgatttg gcttcccggg aacgccccag
    53461 ctgttctgcc aggcgtttca gttccggctg ttcgttaaga aattcaccgt atttcacaat
    53521 caactgatag tcgccacgtt taagctgacc ggcgctcata tcccacagac gaccagctgc
    53581 ggtattgtta tctgcgagaa tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac
    53641 ttcacttaac agttgttcgc gttcttcttc taatagctgt tgatttaacg tcgttgcttg
    53701 cacgatcaga cttaaacgcc aacgctggag gaaaagcgta tgtaacgctg aagtgatcgt
    53761 gctgttagca tcaaccaact gtcgggcttg ttctgcccat ggggaattca gacgatgcag
    53821 taagtccagg atctgtggta gctgcacaat aaactgcggc gtggagagga gctggctttg
    53881 ctgatagcac atcacctctt cggtgagttc cggcgggact cgggcatctt tcagccgact
    53941 gcgcagcgcc tcacgccagc ggggaacatc atcagtgatt gccgccttca gtcgtgggaa
    54001 tttttcaaag aagactgcca gctgcggtga ggccagcagc gcgatgatca tctcttcgat
    54061 caatccctct tcgctgacgg ccagcatcac attaagcgta tccagcgtta gcattgttgt
    54121 gcctggcgaa tctgtgcgcc gacatcctgt aggctggctt caatgcgacc taaccagtcg
    54181 ccagggataa acagacattt ttgctgttcg ctgaataaag cgtgttgctt gcgccagtca
    54241 ctttcaagct cttccagttg ttgttttatt tcacctggca gaccttcagc cgatgaaccg
    54301 ggaagtgcca gcgtactgcc ttgtaaactt acatcgcgta caacaagatg ttgggcgcta
    54361 tccacttcca gattcaattt ctgggcaaag ccgataccgt tcagtttgcc gcgaatttca
    54421 ccacctttac tcagccactg ttccagcgcg ttacgctcaa aggagatatg aaccacttcc
    54481 atatcatgca attttaacgg tttttgcagc agcagagtca gagtggaagc agtaacgtta
    54541 acaggaagtt gatactgctg acgacggctg aaaatgccgc ccagacgaat taccgttaag
    54601 gctgttttat cgctttgttg ctgctggagt tgcaggtgac gttgcacaat cgcgccaagg
    54661 cgagtcaaca tcccttgctg ttgccaggcg tgaccggtca tcaatacatc aatttgttgt
    54721 tgtatcaaat tcaggctttg cgcgtcatac cacaggcaat ctttcagcaa aatgagatct
    54781 accggggcaa cagcactgcg accgctaaaa aaggcgctgg cctgcaataa tcggatcgct
    54841 tttttccagc gacgatccga gacataaggc gcatccggta atttatccag ttgctggcgc
    54901 agcataaaaa tcagctcaaa tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc
    54961 cagcgttcat attcttcatc tgtgacctgc aaggcatcag gaaccggatt gtcgttttca
    55021 tcctgttgac tggtcagcat ggagcggaaa ttcgctttat cctgcacttt atctaaccac
    55081 agacgaatca gcatgcggtc atataacgct tccagactgc tgtctgcttc cggcagctcg
    55141 ttggaggccg ccaccagcag acgcatcggg attttttcta cgtgtgcacc gttgcggaac
    55201 tggcgctcgt taatggcggt gagcaaggta ttaagaattg ccgggcccgc tttccagatc
    55261 tcatccagaa agacaatttc tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc
    55321 ccttcatctt ttagcgcctg aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag
    55381 cgggtcatca gatattcaaa cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg
    55441 atcaaacttt tggcaatacc tggcgggcca aggaggaaca cactttcacc acttaatgcc
    55501 gctaataaac acaagcggat ggcgtggcta cgttcataaa gccccttttc caacgaactg
    55561 ctcaggcggg aaattctttc cgctaataaa tgagggtgag ccataatgaa gtggcgtcct
    55621 ttcgtcaaaa gttctgcgta aattgcgagt atagacgttt cttgctggtg gctaaaatag
    55681 tctcaaaggg ggggtatttt tctttgagcc aggttaatgt ggccgcattt aggagtacga
    55741 ttttgccgtt aatcgtgcat actgtgcgct tttttgtggg ccaagggact aagcacacat
    55801 ttcatatttc aacgaaagac tagtctatga gcactgataa taagcaatca ttgcccgcga
    55861 ttaccctcgc ggcgattgga gttgtctacg gcgatattgg taccagcccg ttatatacac
    55921 ttcgtgaatg tttgtccggc cagtttggtt ttggcgttga acgcgatgcc gtgtttggct
    55981 ttttatcgct gatcttctgg ctgctaatct ttgtggtttc cattaaatat ctcaccttcg
    56041 tgatgcgggc agataacgcc ggtgaggggg ggatcctgac gttgatgtcg cttgccgggc
    56101 gtaatacgtc ggcgcgaacc acatcaatgc tggtgattat ggggctaatc ggcggcagct
    56161 ttttctatgg tgaagtcgtc ataacacccg ctatttcggt gatgtcagcc attgaaggtc
    56221 tggaaatcgt cgccccgcag ctggatacct ggatagttcc cctctcaatt atcgttctca
    56281 ctttattatt tatgattcaa aaacatggca cggctatggt cggtaagctg tttgcgccga
    56341 tcatgctgac ctggtttttg attctggcag gactggggtt acgtagcatt attgctaacc
    56401 cggaagtgct gcatgcgctg aatccaatgt gggcggtgca tttcttcctt gaatacaaaa
    56461 cggtttcttt tattgcatta ggggcagtgg tgttgtcgat tacgggggtc gaggcgctgt
    56521 atgctgatat ggggcacttt ggtaagttcc ctattcgcct ggcgtggttc accgtcgtat
    56581 taccttcctt gacccttaat tacttcggtc agggagcgct gttgttaaag aacccggaag
    56641 cgattaagaa cccgttcttc ctgttggcac cggactgggc gctgatcccg ctgctgatca
    56701 tcgccgcact ggcgacggta attgcctcgc aggcggttat ctctggcgtc ttctcattga
    56761 cgcgtcaggc ggtacgtctg ggatatttgt cgccgatgcg cattattcac acctccgaaa
    56821 tggagtcagg gcaaatctat attccgtttg tgaactggat gctctatgtc gcggtcgtga
    56881 ttgtgattgt cagctttgag cactccagca acctggcggc ggcgtacggg attgcggtga
    56941 ccggaaccat ggtgctgacg tctattctct cgactaccgt ggcacgtcag aactggcact
    57001 ggaataagta ttttgttgcg ctgatcctga ttgctttcct ttgtgtcgat attccattgt
    57061 tcaccgctaa cctcgataaa ctgctctccg gcggctggtt gccattgagc ctcggtactg
    57121 tgatgtttat cgtgatgacc acctggaaga gcgagcgttt ccgcttgctg cggcggatgc
    57181 atgaacatgg taactctctg gaagcgatga ttgcttcgct ggagaaatca ccgcctgttc
    57241 gcgtgcccgg gaccgcggtg tatatgtcgc gtgcaatcaa cgtcattccc tttgcgctga
    57301 tgcataacct taaacataac aaggtattgc atgagcgggt gattctgtta actctgcgta
    57361 ccgaagacgc gccatatgtc cataatgtcc gtcgggtaca gattgaacaa ctgtcgccca
    57421 ctttctggcg cgtggtggca agttatggtt ggcgagaaac gccaaacgtg gaagaagttt
    57481 tccaccgctg cggtctggaa ggattaagtt gccggatgat ggaaacctcc ttctttatgt
    57541 cgcatgagtc gttgatcctc ggcaaacgcc cgtggtattt gcgtctgcgc ggcaagctgt
    57601 acttgctgct gcaacgtaat gcgctgcgtg caccagatca atttgaaatt ccgccaaaca
    57661 gggttatcga actgggtact caggtcgaaa tctaacgcca gacgcctcct ttctccataa
    57721 gggggcgttt ttgttttcat ggttaatcac catgtaaaac gtttcgaggt tgatcacatt
    57781 tccgtaacgt cacgatggtt ttcccaactc agtcaggatt aaactgcggg tcagcgaaac
    57841 gtttcgctga tggagaaaaa aatgaaaaaa ggcaccgttc ttaattctga tatttcatcg
    57901 atgatctccc gtctgggaca taccgatacg ctggtggtgt gtgatgctgg tttacccatc
    57961 cccaaaagta caacgcgtat cgatatggca ttaacccagg gtgtaccttc ttttatgcag
    58021 gtgctgggcg tcgtcacaaa tgaaatgcag gtcgaggctg ccattatcgc ggaagagatc
    58081 aaacaacata atccgcaact ccacgaaacg ttgctcactc accttgagca gctgcaaaaa
    58141 caccagggaa ataccattga aattcgttac accacgcatg aacaattcaa acaacaaacc
    58201 gcagaaagtc aggcggtaat tcgcagcgga gaatgttctc cgtatgcgaa tatcattctc
    58261 tgtgctggcg tgacgttctg aggccgtcat ggaagcatta cttcagctta aaggcatcga
    58321 taaagccttc ccgggcgtaa aagccctctc gggcgcagcg ttaaatgtct atccgggccg
    58381 cgtgatggcg ctggtgggcg aaaacggcgc gggtaaatcc accatgatga aagtgcttac
    58441 tggtatctat gctcgcgatg ccggcacgct tttatggctg gggaaagaaa cgacatttac
    58501 cgggccgaaa tcttcccagg aagccgggat tgggattatc catcaggaac tgaacctgat
    58561 cccgcagttg accattgccg aaaacatttt cctcggtcgt gagtttgtta atcgctttgg
    58621 caaaattgac tggaaaacca tgtatgccga agcggataaa ttgctggcta aacttaacct
    58681 gcgctttaaa agcgacaagc tggtgggcga tctttccatc ggtgaccagc aaatggttga
    58741 aatcgccaaa gtgctgagct ttgagtcgaa agtcatcatt atggatgaac cgaccgatgc
    58801 gctgaccgat accgaaaccg aatccctgtt ccgcgtcata cgcgagctga aatcgcaagg
    58861 ccgcggtatt gtctatatct cccaccgcat gaaagaaatc ttcgagattt gcgatgacgt
    58921 taccgttttt cgtgacgggc aatttattgc tgagcgcgaa gtggcatcac tgaccgaaga
    58981 ttcgctgatt gagatgatgg tgggccgcaa gctagaagat caatatccac acctggacaa
    59041 agcgccggga gatatccgcc tgaaagtcga taatctctgc ggacctggcg ttaacgatgt
    59101 ctcttttact ttacgcaaag gcgaaattct tggcgtctct ggtttgatgg gcgcaggtcg
    59161 taccgaactg atgaaagtgc tctacggcgc actaccgcgc accagcggtt acgtcaccct
    59221 ggatgggcat gaagtcgtta cccgttcacc gcaggatggc ctggcaagcg gcattgtgta
    59281 tatctccgaa gaccgtaaac gtgacggttt agtgttgggc atgtcagtaa aagagaacat
    59341 gtcgctgaca gcgctgcgct acttcagccg cgctggcggc agtttgaagc atgccgatga
    59401 acagcaggct gtgagtgatt tcattcgtct gtttaatgtg aaaacaccgt cgatggaaca
    59461 ggcaattggt ctgctttccg gtggcaatca gcaaaaagtg gcgattgccc gcggtctgat
    59521 gacacgcccc aaagtgttga tccttgatga acctacccgt ggcgtagatg tcggcgcgaa
    59581 aaaagagatc tatcaactga ttaaccagtt caaagccgat ggcttgagca tcattctggt
    59641 gtcatcggag atgccagaag tattaggcat gagcgatcgc atcatcgtca tgcatgaagg
    59701 gcatctcagc ggggaattta ctcgtgagca ggccacccag gaagtgttaa tggctgccgc
    59761 tgtgggcaag cttaatcgcg tgaatcagga gtaaaaaaat gacaacccag actgtctctg
    59821 gtcgccgtta tttcacgaaa gcgtggctga tggagcagaa atcgcttatc gctctgctgg
    59881 tgctgatcgc gattgtctcg acgttaagcc cgaacttttt caccatcaat aacttattca
    59941 atattctcca gcaaacctcg gtgaacgcca ttatggcggt cgggatgacg ctggtgatcc
    60001 tgacgtcggg catcgacttg tctgtaggtt ctctgttggc gctaaccggc gcagttgctg
    60061 catctatcgt cggcattgaa gtcaatgcgc tggtggctgt cgctgccgct ctcgcgttag
    60121 gtgccgcaat tggtgcggta accggggtga ttgtagcgaa aggtcgcgtt caggcgttta
    60181 tcgctacgct ggttatgatg cttttactgc gcggcgtgac catggtttat accaacggta
    60241 gcccagtgaa taccggcttt actgagaacg ccgatctgtt tggctggttt ggtattggtc
    60301 gtccgctggg cgtaccgacg ccagtctgga tcatggggat tgtcttcctc gcggcctggt
    60361 acatgctgca tcacacgcgt ctggggcgtt acatctacgc gctgggcggc aacgaagcgg
    60421 caacgcgtct ttctggtatc aacgtcaata aaatcaaaat catcgtctat tctctttgtg
    60481 gtctgctggc atcgctggcc gggatcattg aagtggcgcg tctctcctcc gcacaaccca
    60541 cggcggggac tggctatgag ctggatgcta ttgctgcggt ggttctgggc ggtacgagtc
    60601 tggcgggcgg aaaaggtcgc attgttggga cgttgatcgg cgcattaatt cttggcttcc
    60661 ttaataatgg attgaatttg ttaggtgttt cctcctatta ccagatgatc gtcaaagcgg
    60721 tggtgatttt gctggcggtg ctggtagaca acaaaaagca gtaataacga ctacaggaca
    60781 tcttgaatat gaacatgaaa aaactggcta ccctggtttc cgctgttgcg ctaagcgcca
    60841 ccgtcagtgc gaatgtgatg gcaaaagaca ccatcgcgct ggtggtctcc acgcttaaca
    60901 acccgttctt tgtatcgctg aaagatggcg cgcagaaaga ggcggataag cttggctata
    60961 acctggtggt gctggactcc cagaacaacc cggcgaaaga gctggcgaac gtgcaggact
    61021 taaccgttcg cggcaccaaa attctgctga ttaacccgac cgactccgac gcagtaggta
    61081 atgctgtgaa gatggctaac caggcgaaca tcccggttat cactcttgac cgccaggcaa
    61141 cgaaaggtga agtggtgagc cacattgctt ctgataacgt actgggtggc aaaatcgccg
    61201 gtgattacat cgcgaagaaa gcgggtgaag gtgccaaagt tatcgagctg caaggcattg
    61261 ctggtacatc cgcagcccgt gaacgtggcg aaggcttcca gcaggccgtt gctgctcaca
    61321 agtttaatgt tcttgccagc cagccagcag attttgatcg cactaaaggt ttgaacgtaa
    61381 tgcagaacct gttgaccgct catccggatg ttcaggctgt attcgcgcag aatgatgaaa
    61441 tggcgctggg cgcgctgcgc gcactgcaaa ctgccggtaa atcggatgtg atggtcgtcg
    61501 gatttgacgg tacaccggat ggcgaaaaag cggtgaatga tggcaaacta gcagcgacta
    61561 tcgctcagct acccgatcag attggcgcga aaggcgtcga aaccgcagat aaagtgctga
    61621 aaggcgagaa agttcaggct aagtatccgg ttgatctgaa actggttgtt aagcagtagt
    61681 tttaatcagg ttgtatgacc tgatggtgac ataaatacgt catcgacaga tgaacgtgta
    61741 atataaagaa aagcagggca cgcgccaccc taacacggtg gcgcatttta tggacatccc
    61801 gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc attaatgctg accacattct
    61861 taatcttcaa tcttttccta ctccaggcga aaccgtaacc ggtaaccact atcaggttgc
    61921 atttggcggc aaaggcgcga atcaggctgt ggctgctggg cgtagcggtg cgaatatcgc
    61981 gtttattgcc tgtacgggtg atgacagcat tggtgagagc gttcgccagc agctcgccac
    62041 tgataacatt gatattactc cggtcagcgt gatcaaaggc gaatcaacag gtgtggcgct
    62101 gatttttgtt aatggcgaag gtgagaatgt catcggtatt catgccggcg ctaatgctgc
    62161 cctttccccg gcgctggtgg aagcgcaacg tgagcgtatt gccaacgcgt cagcattatt
    62221 aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg gcgaaaatcg cccatcaaaa
    62281 taagactatc gttgcgctta acccggctcc ggctcgcgaa cttcctgacg aactgctggc
    62341 gctggtggac attattacgc caaacgaaac ggaagcagaa aagctcaccg gtattcgtgt
    62401 tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg catgaaaaag gtatccgtac
    62461 tgtactgatt actttaggaa gtcgtggtgt atgggctagc gtgaatggtg aaggtcagcg
    62521 cgttcctgga ttccgggtgc aggctgtcga taccattgct gccggagata cctttaacgg
    62581 cgcgttaatc acggcgttgc tggaagaaaa accattgcca gaggcgattc ggtttgccca
    62641 tgctgccgct gcgattgccg taacacgtaa aggcgcacaa ccttccgtac cgtggcgtga
    62701 agagatcgac gcatttttag acaggcagag gtgacgcttg gctacaatga aagatgttgc
    62761 ccgcctggcg ggcgtttcta cctcaacagt ttctcacgtt atcaataaag atcgcttcgt
    62821 cagtgaagcg attactgcca aagttgaagc ggcgattaaa gaactcaatt acgcgccatc
    62881 agccctggcg cgtagcctca aactcaatca aacacatacc attggcatgt tgatcactgc
    62941 cagtaccaat cctttctatt cagaactggt gcgtggcgtt gaacgcagct gcttcgaacg
    63001 cggttatagt ctcgtccttt gcaataccga aggagatgaa cagcggatga atcgcaatct
    63061 ggaaacgctg atgcaaaaac gcgttgatgg cttgctgtta ctgtgcaccg aaacgcatca
    63121 accttcgcgt gaaatcatgc aacgttatcc gacagtgcct actgtgatga tggactgggc
    63181 tccgttcgat ggcgacagcg atcttattca ggataactcg ttgctgggcg gagacttagc
    63241 aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc tgtattaccg gtccgctgga
    63301 taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg gcgatgaaac gtgcgggtct
    63361 caacattcct gatggctatg aagtcactgg tgattttgaa tttaacggcg ggtttgacgc
    63421 catgcgccaa ctgttatcac atccgctgcg tcctcaggcc gtctttaccg gaaatgacgc
    63481 tatggctgtt ggcgtttacc aggcgttata tcaggcagag ttacaggttc cgcaggatat
    63541 cgcggtgatt ggctatgacg atatcgaact ggcaagcttt atgacgccac cattaaccac
    63601 tatccaccaa ccgaaagatg aactggggga gctggcgatt gatgtactca tccatcggat
    63661 aacccagccg acccttcagc aacaacgatt acaacttact ccgattctga tggaacgcgg
    63721 ttcggcttag atttacgctg tcttttgatc aaattattac catcggttgt tttcagaagc
    63781 atgaacattg ctgctgaagc aacagtaata atgcccatcg tgataaacgt atagtggaat
    63841 tgttcgacag tcgttgtgcc ttccatccct tcataaacgc gaaggacggc cgcacttaca
    63901 gcaacgccta aactaatcga cagttgctgc gtgaccgcca gaacactgtt accgctgctg
    63961 gcgttgtcat cggtcagatc ggcaagtgtg atggtattca tcgcggtaaa ttgcgtcgac
    64021 atagccatcc ctaatataaa caacggcaag atcagcatcc atatcgccat tgccggtgat
    64081 tgtaaagaga actgagcgat cattagccca ataatcaccg tgatccccac taacgtatgg
    64141 cgatagccca gacgacgtaa gacttgggta accatcgatt ttgcaataat ggaacctaac
    64201 gctgtcggtg ccatcataca gccggcaata aacgcctgat aaccaaatcc tacctgtaac
    64261 atcaatggca taaggaacgg tacacaaccg gtccccagac gggttgcaat attgcctacg
    64321 ataccgatcg agaaagtgcg ggttttaaat aaatctaatg aaattaatgg gtttggcgtg
    64381 tgtcgcgcat ggagaatata gagaagcagt aacccgatgc tggtgacaat taccgtcaag
    64441 gcaatccagc tggcgacaat cttttccccg aatagctcta ttccgcttga gaagagaaca
    64501 aggctgaggc caaacagcaa aaagccagtg atatcgaatc tgcgtcgtgc ggtggtgaaa
    64561 ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac ctatggggat attgattaaa
    64621 aatatccagt gccaggttgc ccaggtgacc agcacgccgc caagaacggg gcctaaaatt
    64681 ggccccacca gacccggcat ggcgacaaaa ttcaatactg gaagaagttc attacgagga
    64741 taagcgcgca gtaaggccag ccgagcaaca ggcatcatca ttgcgccgcc tatcccctga
    64801 ataacccgga agacaaccag ctgtggtagc gaattagaaa gtgcgcaggc cagagaaccc
    64861 aatgtgaaca gactcacggc aagggtaaaa atgcgacgcg taccgaagcg atcggctagc
    64921 catccgctta ccggaataag catcgccacc gtcagcgtat aactgatgat ggctgattgc
    64981 atcgcgagag gagaacgatt aaggctatga gcgattgcgg gtaaggcggt attcagaata
    65041 gtggcatcaa gtgcctgcat gaagaaggcc atcgccgcga tccacggcaa acccgccata
    65101 ctgcgattct ttttatcgct cattcaatgt cctgttatcg ggttgtcact tatcaggtga
    65161 gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca tcgctttgga caatcgcatc
    65221 gacaatcgcc tggtgcagat ccagctttat cactgtgtcg ctggtaattg acgtgaagta
    65281 agtgtgataa accgaatgga atagcgaggc gaatgaggtc aaaaacggat tggcgctcat
    65341 ttcatagata tgctcatgcc aggccatatc gacttcgatc cagcgttcac ggcgaaagtt
    65401 ctcttttaat gccgccattt cggccattaa cgtattgaga tgcgccttct gttccgcggt
    65461 gccaaccgtt gcagccagta ggcaagcttg cggctccaga cagatacgca taaccagaaa
    65521 gtgatcgatg acctgatgaa agttctcttc tgtcatccac caggtaagca attcctgatc
    65581 aagaaaattc cagtttgatt gtggcatgac ccgggtacca attcgcggtc gcggtaaaac
    65641 catccctttt gccgttaacg ttttgaccgc ttcgcgtacc gctgtacgac tcactccaaa
    65701 ttgctcgccc agctcaatct caccaggcaa aatggtgccg ggttcatatt caccttttaa
    65761 gatgcgttgc gccagcttct cagccagaac atacgaaagg tttttctgtg cagctaactg
    65821 ttgtgcgctt aaaggcatta ctaatcttcc tttttctttt tattcctcct tagtatgcca
    65881 ccaggaagtg tgattacggt tgcaaaaacg gcaaattgct tgttttatgg cacattaacg
    65941 ggggttttgc tgaaaaaatg cgcggtcaga aaattatttt aaatttcctc ttgtcaggcc
    66001 ggaataactc cctataatgc gccaccactg acacggaaca acg
//
LOCUS       DAEVZA010000008        65546 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEVZA010000008 DAEVZA010000000
VERSION     DAEVZA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.008"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..969
                     /locus_tag="LAF92_000565"
     CDS             <1..969
                     /locus_tag="LAF92_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBI9789624.1"
                     /translation="KVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETH
                     NGVVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQ
                     EGLSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSG
                     NQGITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGA
                     AAGMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALD
                     DTAVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(1181..1345)
                     /locus_tag="LAF92_000566"
     CDS             complement(1181..1345)
                     /locus_tag="LAF92_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789625.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1368..2069)
                     /locus_tag="LAF92_000567"
     CDS             complement(1368..2069)
                     /locus_tag="LAF92_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBI9789626.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2174..3070
                     /gene="yhaJ"
                     /locus_tag="LAF92_000568"
     CDS             2174..3070
                     /gene="yhaJ"
                     /locus_tag="LAF92_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBI9789627.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            complement(3121..3477)
                     /locus_tag="LAF92_000569"
     CDS             complement(3121..3477)
                     /locus_tag="LAF92_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBI9789628.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(3719..4084)
                     /locus_tag="LAF92_000570"
     CDS             complement(3719..4084)
                     /locus_tag="LAF92_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBI9789629.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(4377..5363)
                     /gene="yqjG"
                     /locus_tag="LAF92_000571"
     CDS             complement(4377..5363)
                     /gene="yqjG"
                     /locus_tag="LAF92_000571"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBI9789630.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(5433..5825)
                     /locus_tag="LAF92_000572"
     CDS             complement(5433..5825)
                     /locus_tag="LAF92_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBI9789631.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(6011..6310)
                     /locus_tag="LAF92_000573"
     CDS             complement(6011..6310)
                     /locus_tag="LAF92_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBI9789632.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(6300..6704)
                     /locus_tag="LAF92_000574"
     CDS             complement(6300..6704)
                     /locus_tag="LAF92_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBI9789633.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(6707..7012)
                     /locus_tag="LAF92_000575"
     CDS             complement(6707..7012)
                     /locus_tag="LAF92_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBI9789634.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(7050..7418)
                     /locus_tag="LAF92_000576"
     CDS             complement(7050..7418)
                     /locus_tag="LAF92_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBI9789635.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(7565..7948)
                     /gene="mzrA"
                     /locus_tag="LAF92_000577"
     CDS             complement(7565..7948)
                     /gene="mzrA"
                     /locus_tag="LAF92_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBI9789636.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(7952..8614)
                     /locus_tag="LAF92_000578"
     CDS             complement(7952..8614)
                     /locus_tag="LAF92_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI9789637.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(8959..9735)
                     /gene="exuR"
                     /locus_tag="LAF92_000579"
     CDS             complement(8959..9735)
                     /gene="exuR"
                     /locus_tag="LAF92_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBI9789638.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            10031..10747
                     /locus_tag="LAF92_000580"
     CDS             10031..10747
                     /locus_tag="LAF92_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI9789639.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            10777..11277
                     /locus_tag="LAF92_000581"
     CDS             10777..11277
                     /locus_tag="LAF92_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9789640.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            11369..14053
                     /locus_tag="LAF92_000582"
     CDS             11369..14053
                     /locus_tag="LAF92_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBI9789641.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKVFSEQDEDSQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            14050..15138
                     /locus_tag="LAF92_000583"
     CDS             14050..15138
                     /locus_tag="LAF92_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBI9789642.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(15226..16524)
                     /gene="exuT"
                     /locus_tag="LAF92_000584"
     CDS             complement(15226..16524)
                     /gene="exuT"
                     /locus_tag="LAF92_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBI9789643.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            17007..18419
                     /gene="uxaC"
                     /locus_tag="LAF92_000585"
     CDS             17007..18419
                     /gene="uxaC"
                     /locus_tag="LAF92_000585"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBI9789644.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            18434..19921
                     /gene="uxaA"
                     /locus_tag="LAF92_000586"
     CDS             18434..19921
                     /gene="uxaA"
                     /locus_tag="LAF92_000586"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBI9789645.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGVHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            20004..20555
                     /locus_tag="LAF92_000587"
     CDS             20004..20555
                     /locus_tag="LAF92_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBI9789646.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(20560..21804)
                     /gene="sstT"
                     /locus_tag="LAF92_000588"
     CDS             complement(20560..21804)
                     /gene="sstT"
                     /locus_tag="LAF92_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBI9789647.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(22009..22311)
                     /locus_tag="LAF92_000589"
     CDS             complement(22009..22311)
                     /locus_tag="LAF92_000589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789648.1"
                     /translation="MASKSPKRSLEAGATRMDARRGRILQGRYLAPDPVAARDKLREP
                     RSGDFVRQSLGVQGAAAIGRPLRAPCASDFIWHNTLKERNRSHIPNSFAHMLSNQP"
     gene            complement(22420..23385)
                     /gene="alx"
                     /locus_tag="LAF92_000590"
     CDS             complement(22420..23385)
                     /gene="alx"
                     /locus_tag="LAF92_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBI9789649.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(23668..24654)
                     /locus_tag="LAF92_000591"
     CDS             complement(23668..24654)
                     /locus_tag="LAF92_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI9789650.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(24733..25416)
                     /locus_tag="LAF92_000592"
     CDS             complement(24733..25416)
                     /locus_tag="LAF92_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBI9789651.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(25493..25996)
                     /locus_tag="LAF92_000593"
     CDS             complement(25493..25996)
                     /locus_tag="LAF92_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBI9789652.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            26081..27217
                     /gene="rlmG"
                     /locus_tag="LAF92_000594"
     CDS             26081..27217
                     /gene="rlmG"
                     /locus_tag="LAF92_000594"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBI9789653.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            27408..27491
                     /locus_tag="LAF92_000595"
                     /pseudo
     CDS             27408..27491
                     /locus_tag="LAF92_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            27488..27904
                     /gene="higA"
                     /locus_tag="LAF92_000596"
     CDS             27488..27904
                     /gene="higA"
                     /locus_tag="LAF92_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBI9789654.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(27949..29967)
                     /gene="fadH"
                     /locus_tag="LAF92_000597"
     CDS             complement(27949..29967)
                     /gene="fadH"
                     /locus_tag="LAF92_000597"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBI9789655.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(30393..32744)
                     /gene="ygjK"
                     /locus_tag="LAF92_000598"
     CDS             complement(30393..32744)
                     /gene="ygjK"
                     /locus_tag="LAF92_000598"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBI9789656.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(32761..33831)
                     /gene="ygjJ"
                     /locus_tag="LAF92_000599"
     CDS             complement(32761..33831)
                     /gene="ygjJ"
                     /locus_tag="LAF92_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HBI9789657.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(33965..35398)
                     /locus_tag="LAF92_000600"
     CDS             complement(33965..35398)
                     /locus_tag="LAF92_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI9789658.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDSMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(35461..35910)
                     /locus_tag="LAF92_000601"
     CDS             complement(35461..35910)
                     /locus_tag="LAF92_000601"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBI9789659.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(35907..38999)
                     /gene="ebgA"
                     /locus_tag="LAF92_000602"
     CDS             complement(35907..38999)
                     /gene="ebgA"
                     /locus_tag="LAF92_000602"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBI9789660.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(39183..40166)
                     /gene="ebgR"
                     /locus_tag="LAF92_000603"
     CDS             complement(39183..40166)
                     /gene="ebgR"
                     /locus_tag="LAF92_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBI9789661.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            40385..40717
                     /locus_tag="LAF92_000604"
     CDS             40385..40717
                     /locus_tag="LAF92_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBI9789662.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(40759..42138)
                     /gene="ygjG"
                     /locus_tag="LAF92_000605"
     CDS             complement(40759..42138)
                     /gene="ygjG"
                     /locus_tag="LAF92_000605"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBI9789663.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            42616..44076
                     /gene="aer"
                     /locus_tag="LAF92_000606"
     CDS             42616..44076
                     /gene="aer"
                     /locus_tag="LAF92_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HBI9789664.1"
                     /translation="MSTTDLQSYITHANDTFVQVSGFTLQELQGQPHNMVRHPDMPKA
                     AFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPMVREGKISGYMSIRTRATDEE
                     IAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLPLRWRARGVMTLMFILLAAML
                     WFVAAPVVTYFLCVLVVLLASTCFEWQIVRPIENVARQALKVATGERNSVEHLNRSDE
                     LGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAKGTDELNEHTQQTVDNVQQTV
                     ATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAMTTVIKTMDDIADSTQRIGTI
                     TSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGEVRHLASRSANAANDIRKLID
                     ASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQISHSTLEQADGLSSLTRAVDEL
                     NLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(44230..44853)
                     /gene="nfeR"
                     /locus_tag="LAF92_000607"
                     /gene_synonym="yqjI"
     CDS             complement(44230..44853)
                     /gene="nfeR"
                     /locus_tag="LAF92_000607"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBI9789665.1"
                     /translation="MSHHHEGCCKHEGQPRHEDCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            45130..45894
                     /gene="nfeF"
                     /locus_tag="LAF92_000608"
     CDS             45130..45894
                     /gene="nfeF"
                     /locus_tag="LAF92_000608"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBI9789666.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(45948..46023)
                     /locus_tag="LAF92_000609"
     tRNA            complement(45948..46023)
                     /locus_tag="LAF92_000609"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45988..45990),aa:Ile,seq:cat)
     gene            46148..46654
                     /gene="mug"
                     /locus_tag="LAF92_000610"
     CDS             46148..46654
                     /gene="mug"
                     /locus_tag="LAF92_000610"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBI9789667.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKRAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(46733..48574)
                     /gene="rpoD"
                     /locus_tag="LAF92_000611"
     CDS             complement(46733..48574)
                     /gene="rpoD"
                     /locus_tag="LAF92_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBI9789668.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(48769..50514)
                     /gene="dnaG"
                     /locus_tag="LAF92_000612"
     CDS             complement(48769..50514)
                     /gene="dnaG"
                     /locus_tag="LAF92_000612"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBI9789669.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(50625..50840)
                     /gene="rpsU"
                     /locus_tag="LAF92_000613"
     CDS             complement(50625..50840)
                     /gene="rpsU"
                     /locus_tag="LAF92_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBI9789670.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            51077..52090
                     /gene="tsaD"
                     /locus_tag="LAF92_000614"
     CDS             51077..52090
                     /gene="tsaD"
                     /locus_tag="LAF92_000614"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBI9789671.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(52133..53596)
                     /gene="ttdT"
                     /locus_tag="LAF92_000615"
     CDS             complement(52133..53596)
                     /gene="ttdT"
                     /locus_tag="LAF92_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBI9789672.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(53644..54249)
                     /gene="ttdB"
                     /locus_tag="LAF92_000616"
     CDS             complement(53644..54249)
                     /gene="ttdB"
                     /locus_tag="LAF92_000616"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBI9789673.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(54246..55157)
                     /gene="ttdA"
                     /locus_tag="LAF92_000617"
     CDS             complement(54246..55157)
                     /gene="ttdA"
                     /locus_tag="LAF92_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBI9789674.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            55364..56296
                     /gene="ttdR"
                     /locus_tag="LAF92_000618"
                     /gene_synonym="dan"
     CDS             55364..56296
                     /gene="ttdR"
                     /locus_tag="LAF92_000618"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HBI9789675.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(56309..56926)
                     /gene="plsY"
                     /locus_tag="LAF92_000619"
     CDS             complement(56309..56926)
                     /gene="plsY"
                     /locus_tag="LAF92_000619"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBI9789676.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            57031..57399
                     /gene="folB"
                     /locus_tag="LAF92_000620"
     CDS             57031..57399
                     /gene="folB"
                     /locus_tag="LAF92_000620"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBI9789677.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            57489..58310
                     /gene="bacA"
                     /locus_tag="LAF92_000621"
     CDS             57489..58310
                     /gene="bacA"
                     /locus_tag="LAF92_000621"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBI9789678.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(58473..59711)
                     /gene="cca"
                     /locus_tag="LAF92_000622"
     CDS             complement(58473..59711)
                     /gene="cca"
                     /locus_tag="LAF92_000622"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBI9789679.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(59775..60395)
                     /locus_tag="LAF92_000623"
     CDS             complement(59775..60395)
                     /locus_tag="LAF92_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI9789680.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            60637..61938
                     /gene="ygiF"
                     /locus_tag="LAF92_000624"
     CDS             60637..61938
                     /gene="ygiF"
                     /locus_tag="LAF92_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBI9789681.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            61961..64801
                     /gene="glnE"
                     /locus_tag="LAF92_000625"
     CDS             61961..64801
                     /gene="glnE"
                     /locus_tag="LAF92_000625"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBI9789682.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            64849..>65546
                     /locus_tag="LAF92_000626"
     CDS             64849..>65546
                     /locus_tag="LAF92_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="catalyzes the phosphorylation of
                     D-glycero-D-manno-heptose 7-phosphate to form
                     D,D-heptose-1,7-bisphosphate and catalyzes transfer of ADP
                     to D-glycero-D-manno-heptose 1-phosphate forming
                     ADP-D,D-heptose; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HBI9789683.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRS"
BASE COUNT        15792 a        16630 c        17405 g        15719 t
ORIGIN      
        1 aaagtctccg ttaagatcca ggaaccttgc aatgaaatcc tcttctcacg cgccaaagtc
       61 tggaacggtg agaagtgggc gtgtgtcacc atcgtcggcg ggcataccaa cattgtgcat
      121 attgagacgc acaatggtgt ggtgtttacc cagcaggcgt gtgtggcaga gggcgagcaa
      181 gagtctccgc tgacggtgct ttccagaacg acgctggctg agatcctgaa gttcgtcaat
      241 gaagtcccgt ttgcggcgat ccgctttatt ctcgattccg cgaagctaaa ttgtgcgtta
      301 tcgcaggaag gtttgagcgg taagtggggg ctgcatattg gcgcgacgct ggaaaaacag
      361 tgcgagcgcg gtttgctggc gaaagatctc tcttcatcca ttgtgattcg taccagcgcg
      421 gcatccgatg cgcgtatggg cggcgctacg cttccggcta tgagtaactc cggctcgggt
      481 aaccagggga tcaccgcaac aatgcctgtg gtggtggtag cagaacactt cggagcggat
      541 gatgaacggc tggcgcgtgc gctgatgctt tctcatttga gcgcaattta catccataac
      601 cagttaccgc gtttgtctgc actttgtgcc gcaacgaccg cagcaatggg ggccgccgcc
      661 gggatggcat ggctggtgga tgggcgttat gaaaccatct cgatggcgat cagcagtatg
      721 atcggcgatg tcagcggcat gatttgcgat ggtgcgtcga acagctgcgc gatgaaggtt
      781 tcgaccagtg cttcggctgc gtggaaagcg gtgttaatgg cgctggatga taccgccgtg
      841 accggcaatg aagggatcgt ggcgcatgat gttgagcagt cgattgccaa cctgtgtgcg
      901 ttagcaagcc attcgatgca gcaaacggat cggcagatta tcgagattat ggcgagcaag
      961 gccagataaa cgccaggttt tgagtattgt gccggatgcg atgctggcgc accttatccg
     1021 gcctacgggg tgctcaactt gttgatgttg ttgtgttttg tacctgatgc cggatgcgat
     1081 gctggcgcat cttatccggc ctacgggttg tttaattcgt taatgttgtt ggattttgta
     1141 ggccggataa ggcgttttcg ccgcatccgg caacctgtct ttacgcggta gcttcatcct
     1201 cggctaaacg cgtttcagca tccgcgacga tggcttccag ctcgctcaac atctcttcat
     1261 agccaaaatt tttggcggtg cgagcaggtt ctttcgccac cacgggcttc actggctggc
     1321 gctttttggc cgatttttta ctcatcatca tctccctgta aaaacaacta gacaggcaaa
     1381 tctatcagca aagcgcgcag tggggaatcg gcaaccagcg taatgttagc ctcgtcacga
     1441 ataaacgccc catcaccgca ggtcagcgcc gctttctctt catgatgcgt aagcgcatga
     1501 aatttcccgt gaatcgattg caaatacgcg cgtggcccat gcaactggaa attcgcactt
     1561 tcgcctttgt cgagcacgat atggtgcagc cacacttgct ggcgtaattg caggcttccc
     1621 atcgtaccct ctggcgaggc gattaattgc tgcttgccca tattaagcgc cagtttttga
     1681 atcagcggat tctctcgctg cgggcaggcg tccagccaaa gctgcattcg tgttaacggt
     1741 ttgtctttgc tgagattgtg ttcgctgtag ctgacacccg gctgggtaga gagcagcaac
     1801 gcctcaccgg cgctggcctg aacatgattg ccttcgctgt cgcgatactc tgcttctcca
     1861 tccagaatca catttaaaat atcgactttg ggataggttc gcggctgaaa ggcggcacct
     1921 ggggccagca cttcctggtt aagcacacgc agggaggcat agcctaacaa tttcgggtcg
     1981 aagtagtgtc caaaggaaaa agtataccgg gcctgcaacc atccgtagtc tgcttgtcca
     2041 cactgtctgg cagttcgggt agtaatcata attcttgacc tctctttact tccttttatg
     2101 gtaaggggct ggacgctaca ttgttagcca gatattctgc ccggtatgtt caaatttcct
     2161 gaatgagaac gaaatggcca aagaaagggc attaacgctg gaagcactac gggttatgga
     2221 tgcgatcgat cgccggggca gttttgcggc ggcggcggat gagctgggac gcgtgccttc
     2281 cgcacttagc tacaccatgc aaaaactgga agaagagctg gatgtggtgc tgtttgaccg
     2341 ctcgggccat cgcaccaaat tcaccaatgt cgggaggatg ttgctggagc gagggcgcgt
     2401 tttgctggaa gccgcagata aactgactac cgatgcggaa gccctcgcgc gcggttggga
     2461 aacgcatctc accattgtga ccgaggcgct ggtaccgaca cctgcctttt tcccgttaat
     2521 cgacaaactg gcggcaaaag ccaataccca actggcaatc atcaccgaag tgctggcggg
     2581 ggcgtgggaa cgactggagc aggggcgggc ggatatcgtt atcgcgccgg atatgcattt
     2641 tcgttcctcg tcggagatca actcgcgcaa gctctatacg ttaatgaacg tctacgttgc
     2701 cgcgcctgat cacccgattc atcaggagcc ggaaccgtta tctgaagtga cgcgcgtgaa
     2761 atatcgtgga attgcggtag cggataccgc tcgtgagcgc ccggtgttga ccgtacagct
     2821 gctggacaaa cagccgcgct taacggtaag cacgattgaa gataaacgtc aggcattact
     2881 ggcggggctt ggcgtggcga cgatgccgta tccgatggtc gaaaaagata ttgcggaagg
     2941 gcggttgcgt gtcgtcagcc cggaatcgac cagcgagatc gatattatta tggcctggcg
     3001 tcgtgacagt atgggggaag cgaaatcctg gtgtctgcgg gaaattccca aactttttag
     3061 cggaaaataa tatttgttat tattaataaa aaggggcgaa atgtcgcccc aattctaaga
     3121 ttaatttgaa ccaaacttag gatcgtttcc gtaacgattg ctgccagaat tgccttctaa
     3181 acaggcaaat acaaacagaa ctaaccagcc aatgatcggt accagataga gaagcgccca
     3241 cgcacctgaa cggtcagtat cgtgcagacg acgaacacac agagcaatga ccggaatgat
     3301 tgttgctgcc aggtaaatga gagaaaggaa tggaaactct aaacctaaaa tcaattgaat
     3361 gacattgata atagcgccga cgatggcgtt aatcagagta aacatccagt actctttacg
     3421 acgtgcgcga ccagagaaac caacataatt ttttaatacg gctaaatacc actgcataaa
     3481 aacatccctt taattaaaag tgacgaaagt ctattccggg tattgtgtaa atgctattcg
     3541 tatctttctt tttcggatgt tataaaggtt ttgatattta agataaatac atggttgttt
     3601 tttataggta atttatataa cgacattgaa atattttaaa taaacattag taaattattc
     3661 attcatcaac atgatgataa aagccggatg agtcagacat ccggcgagca gatttgtttt
     3721 aaggttccag tttcggatct ggtccaaagc ggttttcgcc tggcgtaccc gcctggcagt
     3781 taaagacgat gataataagc cagccgataa acgggattaa gaacaacagt gcccaccacg
     3841 ccgagcggtc ggtgtcgtgc aggcggcgga actgaactgc ccaccacggt aaaaacacca
     3901 gaataccgta gattgtcgtc aggatgcctt cgccaccagc gcgttgccag cctaacattt
     3961 tatccagcag ccccagcacg aacgtaaaga tgatgttgac cagaataaac atccagtact
     4021 ctttgcgccg cgcacgaccc cggaaaccga cataattttt tagtactttc agataccagt
     4081 ccatttttgc ctcaattttg ctcaatcact tgtttttaaa gcagcaaatt aagaatagac
     4141 gtgaatgaaa tggcagtaaa tattcgttca aggaattaat aaagtgctgt gaaggcgcgc
     4201 taagactgat gacaagcgca aaatcgccag atgcgctacg cttatctggc ctgcgcggtc
     4261 attgcaacat attgaatata aatgttttgt aggtttgata aggcgttcac gccgcatccg
     4321 acatgaacaa catgcactta gctaattatc agaggcacct cgtaaggtgc ctttttttaa
     4381 ccgaagcgaa catctcgtcc atgcggttca tcgagatcct gccacggacc aattgaaata
     4441 atccccgtag ggttgatggt cttatggctg cggaagtaat gattacggat gtgatcgaaa
     4501 ttgacagttt cggcgatccc cggcatctgg tagatatcgc gcaggaagcc atacagattc
     4561 aggtaatcgc tgatgcggtg cttatcacac ttgaagtggg tcacatacac tggatcaaaa
     4621 cgcaccagcg tagtccacag gcgaatatcg gcttcggtta gctggttgcc ggtcaggtaa
     4681 cgatgctggc ctaaaatctg ctccagtcgc gccagcgatt caaacacttt cgccaccgcc
     4741 tcgtcgtagg cttgctggct ggtggcaaaa ccagctttat acacgccgtt gttaacggtg
     4801 tcataaatcc agccgttaag ttcgtcaatt ttcgtttgca gggctggtgg gtagtaatca
     4861 cccgcttttg cgcccagcgc atcaaacgcg gtattgaaca tgcggatgat ttctgctgat
     4921 tcgttgctga cgatggtgtg gttctttttg tcccacagca cgggaacagt aactcgtccg
     4981 ctgtagtgtg gatcggcgtg gagataaagc tgatacagaa attcatgttg atagagcgta
     5041 tctccggttg ctcccggaaa actgtcatca aaggtccagc cgttttccag catcagcggg
     5101 ttcactacgg aaacggaaat aaacggttcc agtcctttga gtttgcgcat gatcagcgtg
     5161 cggtgcgccc acgggcaggc gagtgaaaca tagagatgat aacgatcttt ctctgcgata
     5221 aaaccgcctg tgccagtggg gccaggtgcg ccatcggcag tgagccagtt acgaaatgcg
     5281 gaagctgaac gttgaaattt accgccggta gatttggtat cgtaccaggt gtcatgccag
     5341 acgccgtcaa tcagttgccc catttttctc tcctccaata gcaaaaacga ggagcttcct
     5401 cctcgttttt tatcgttaag tatagcgcgt gcttaccact ttttattcag caggcggtcg
     5461 atgctatacg cgcccggacc ggtaattgcc agcagcagga atccgccaga aattgtcagg
     5521 tttttcatga acatcagcga gttgacgcct tcagcaaagt tgctgtgaaa taaaaatgcc
     5581 gtcagcagcg taaagcccgc agtaaacagg gctgtggtgc gagtcaggaa accgaacagg
     5641 attgccagac caccaccgaa ctcaagcaga atcaccagcg gcagcataaa acccgggaca
     5701 cccattgctt ccatatattg ttgggtacca gagtaagcgg tgatttttcc ccagcctgcg
     5761 gtaataaaca gaatcggcat taaaatgcgc gctaccagta caccaacatc ttctaatttt
     5821 ttcatcatac tctccactgc gccactcagg ctgctgattg tttttttgtg caagggcgcg
     5881 gtattagcgt cgttgctgtc gatggagaga atcataaacg tggtgaatga tgattgttag
     5941 caaggaaaac tgtcaaaaat cttcaagaaa tttgagggat aaggccggaa ttgctccggc
     6001 cagagggaag ttaaccgcga agctgttgct gcttgagggt cgttttaacc agacgccagg
     6061 cgctccatac gccaaaaccg cgtctggccc agcggaccag catattagga tggcgaatcg
     6121 tccagatcgc catcacgcta ctgccaacca gcgcccagga gcgcagactt agcagcatat
     6181 tccagcgacg atcgtaagcg cctgttgcct ccagccattc acgacgactg gcggaaagat
     6241 ccagccgttg ttgctggatc tggctaagca gttgcgcctt acgtcgttca cgttcgactt
     6301 tactgctcac gggactcctc ctcgagcagt tgccgatcgt ttgccagctc atggcgtgta
     6361 tggcgcagca aggtggattt acgcgattta cgtaacgtcc agataccgcc aattagtgcc
     6421 agtagcagca acaccacggt ggtggcaatc atcgcattca ggcgatattg cggatcaacc
     6481 gcccaaataa ttagcaccat caggctcata agaccaaatg cagcgaaaag catcgtcagg
     6541 cccagcatca gtaaaagttg aaagagatta gctttttcct cttccagctc caccaccgcc
     6601 agccgcagac gtgtttccac catttcaacc atgatggaaa caattcgctg cccgatgccc
     6661 agaacgcttt taccgggccc ttgcgcgtga tgagtgtccg ccataattaa cgacgcgaca
     6721 gcagaacgcc gagcactaca ccgattgcag cgccaatgcc cacgccggtc cacggatttt
     6781 cacgcacata ctcatcagca cgcgccgccg cgacacgggt ttgtttggcg atggcatcac
     6841 cggtttcacc caggcgataa cggctctgtt tcagtgcctg ctccgctttg ctacgaatct
     6901 tactcaactc ttctttcgac ttctcgccag atgagctaag cacctcttcc agcgtatcgg
     6961 aaagggattt caactcagca cgcagatgtt ccgtagtgtg ttctttcgac atagttttct
     7021 ccaggtgagt aagtagtgac tattgtgagt tagtagtcgc gcgcttccag ctttttcagc
     7081 tcttcctgcg cttctgccag tttccgttcg cgtttggcaa tcttatcggc atcgcctttt
     7141 tgcttcgcct cggctaaatc ttgctggcgt tccgccacct catctttctg ctttgcgatt
     7201 ttcttgtgat gatcggcacg cagctggcta tctgaacagt tggcccggac ttcactcagg
     7261 gctttattca gaccgtcaat acgattctgg ttttggtgtt tttcggcata gctgatctcc
     7321 ttaaggatat tttgctcctt ttcctgacac agggtagtgg cataactacc ggcactaaga
     7381 gcaaagagag aaacagctaa agcgatgcgg tatttcattc ttgaaccttc cattgtgatt
     7441 cggacctatt acaaaggaac gattgtccag acaacgaagc ggtagtgtta accaccgcct
     7501 cattaatcat agacagttac ggtgaaatca ccaaagtgag tgatatgttt cggataacag
     7561 gaagttatcc gaagcgatga gagttatccc gtaaccgggt cagccactgc atagcctgac
     7621 tgttattgtc ctgctgcgca atgatgtagc catggggcaa tgttctgtcg aggaccgctt
     7681 ttgcggcggc gctctggtcg ctggagtcaa atgtaattaa cagagtgtcg tttttggggg
     7741 tgatactttt gaaaggaatg ccatgagcgt caaggtgatg ccagatcgaa aaaccgtctg
     7801 gcatcgttgt gccttgatga acggcacgaa tcgccagcgt agactcttgc tggcgaaccg
     7861 cggaccaggc cagcaacagc gtgccgacca gaagtaaaac agtgccggac caggctagct
     7921 ggcgaagcga catgcgaggt atttgcatcc cttacccccg atttccatat ttctttttcc
     7981 ataacacgac cagagaacct gccaggccaa acaccagcag caccaccggg agcagcatca
     8041 ggcatgacat cagctggtcc tcgtacttta aaaataccgg cgttttgccg agcatgtaac
     8101 ccagagttgt caggatcaat acccacagca gaccgctcat ccagttgaaa aactgaaagc
     8161 gcgcgttatt cagccctgat aacccggcaa tcgtcggcag cagtgttctg acaaacgcaa
     8221 taaagcgacc aattaacagc gccgataaac cgtgtttatg aaaaagatgg tgtgcgcgtt
     8281 gatgataatg cgcgggtaaa tgagatagcc agttttgtac ggtgcgggta ttgcccagcc
     8341 atcgcccctg aatatagctg acccagcagc cgaggctggc ggcaacggtc agcagcagaa
     8401 tcgtttgcgg gtaacccatc gcgcctttcg caatcaacac gccgaccaat accagtaaac
     8461 tgtcgcccgg taaaaaggcc gctggaagca agccgttttc aaggaacaaa attacaaaca
     8521 agacaaaata caacatgcca atcatcgatg gattggccag ggtttcaaaa tcctgcgccc
     8581 acagggcttg cagcaattgg gtcaaaagtt ccattcatta ttcctggtga atcggttaac
     8641 gccacgctgg ttttaaaaga tgcactaccg caatgttatt gctgttttgg tatgttctgc
     8701 gggtttgata catcaacaca gcctgaacta ttccctgtta aatcgtgaga taagaaagca
     8761 ttcaaaaaca taaattcaaa ttgaatctaa ttgtaacaaa ggtcaacgtt gttcgttatc
     8821 aaaattacat gtccgtggag tctgattttg ctgaatgcag gggagggtgg cgagaggatt
     8881 tacacaggct gacactttat acttttcgct taccgacgag acaaacaggc gcgtcgcagc
     8941 gcctgtcgga caggtatttc atttactgcc actggtggcc gtatcgagat gcaccaccgg
     9001 gttttcggcg aatagatagc ggtcggcatt gaactcgaag tcgtcgctgg tttcgttgaa
     9061 taacatgatc ttggtgtttt ccaggtgctg ccacattgcc agcttagcag catgaggatc
     9121 tttgcgaatc agcgccttga ggatttgatc gtggtcatca caccagttat cgacggtacg
     9181 ggaatcaatg tgttcgtgca gttttttcca gtacgggtta tggctacgtt gtgtccacat
     9241 tttttcaacg atggccgcca gggcggagtt ctgcgtcgcc agggctacct gaatgtggaa
     9301 ctgtaaatcc cactcggaat cacggaagca ttgttcgccg cgcgcctgtt cctgaatggc
     9361 catcagtttc atgatgtcct gtttcgttac ctgagttgcc gcgaattcgg caatattact
     9421 ttcgatgagc tggcgagcct gaagcaactc aaacggaccg taattggcga attccatatt
     9481 attgtcagct gcctgctgat ggcgcggctg gttggaaacc acatgaatac ctgaaccttt
     9541 acgcacttcg acatagcctt caacttccag catgatgatg gcttcacgaa ctaccgtacg
     9601 gctgacgttc ttttcatcgg caataaagcg ttctgcaggc agtttatcac ctaccagata
     9661 gacgccctgt tcgatgcgct ctttcaggtc agcggcaagt tgttgataca aacggcgtgg
     9721 ttcagtgatt tccatatgcg tctcccggag tggaatgcgg acgaagttat cataccactt
     9781 tattcatggg tatgccattg gtcatatata aatttggtga ttgaattaat gtattgaatt
     9841 aatattgtga atatttataa attcctaaaa aataagtatc gtcattgtga aagctactaa
     9901 atttcagtaa gttaattccc agcctttaaa tttaatatta actaaaaatg cttttttata
     9961 ttgacttaca ataaatctac atctctaata caccaacttt ttggaaacaa tattgtaaga
    10021 tcaaatttat atggtggcga aaattaatct cttatcacgg cttacgccgc ttttattcgt
    10081 gttcgctcct tttttggcac agtcaaatat gactgtttat cctatggctg tttccattaa
    10141 tagtcagggt gaaggcaacg ttcgcgtaat atctaaaagt aatgaagttc agtacatcaa
    10201 ggcgacagta ttccgtatcg ataatccatc gacgcctcag gaaaatgaag ttgagattaa
    10261 atccggtgat gcaaatcatc tggttgttat gccacctaaa ttcgctttac cagccggtag
    10321 tagcaaaacc gtacgttttg ttgcgatgga accagagcaa aaagagaaaa attatcgcgt
    10381 taaatttgaa gcggttccca gtattgatga cgttgccaca gataaaaaag atctctctat
    10441 gcagttaaca gttaacttaa tttgggggat tgttgttagt gttccacctc agcaacctat
    10501 tgctaagtta gaagtaaatg ctgcccaaaa attagttaat gcagggaatc aacgcttaaa
    10561 gattttaacg atcgcttatt gtaaaaataa tagcaaagaa aattgtaaga tacagaccgt
    10621 aaataaaaac atcttccctg gtcaggaaag aaatcttgaa agcatatctg gctacgacaa
    10681 aatcgttgtc aaatataata actggatcac caaagataat ggcgagtttg aactggcggt
    10741 ccattaatta tcattgtaat aaacgaagga atagtaatga aaaaggtatt tgcaaaatct
    10801 cttctggtcg cagcgatgtt ttctgttgca ggctccgcat tggcagtaca aaaggatatt
    10861 actgtaacgg ccaacgttga tgccgctctg gatatgacac agaccgataa caccgcgttg
    10921 ccgaaagcag tagaaatgca atatctgccg gggcagggtc tgcaatctta ccagttgatg
    10981 accaaaatct ggtctaacga cgttaccaaa gatgtaaaaa tgcagttagt ttccccggcg
    11041 caactggttc aaagcctgga tgctagcaag attgttccgc tgacagtaac ctggggcggt
    11101 gaagaaatta aagctgatgc ggcaaccact tttaccgcaa ccaaaatctt tgcttccgat
    11161 gcgttaacta acggttctct ggctaaaaac ctgatgtttg ctcaaaccac caaaggtgtt
    11221 ctggagacag gcatctaccg tggtgtagta agcatttatc tgtctcagga tatctaatcc
    11281 agataacaga attaacacgt aacaaaggga ggggtagccc tcccttattt taactgtttt
    11341 agtatttgtt atggatttca ggattgccat ggataaaaaa ttactggctc ttttgatcct
    11401 ggcgagtctc agcccggcag aggcgacatt aaccaaaatt cccgcagggt ttgaggttat
    11461 tgctcaggga cagcaggagt atatcgaggt ttatttttca gggaaaagtc tcggtaaata
    11521 ttatgcaatg gttaatcttg ataccgtaac atttcttgat ccagcaagtc tatataacaa
    11581 gctggaactg gatgtagacg atcagaaaat cgcgcatata gtgaaagaaa aattatcgca
    11641 gccgctagct cgccacggtg aattggcttg cggttatgta cgtactgact cagggtgtgg
    11701 ttttctgaat accgatacgc tggaaataat ctataatgat gaagaaagtt cggcaacgtt
    11761 gtttattaat ccgcaatgga attcagcttt cgatgcgaag tcattatatt taaatccaga
    11821 caaaaatact gttaatgctt ttatacatca gcaagacatc aatgttctgg cacaggatga
    11881 ttaccaatcg ttgtctattc agggaaacgg tgcgctggga ataacagaaa atagttatat
    11941 tggtgcacac tggaatttca acggttatga tgcagatgat gtcagtgaca gtaatgctga
    12001 cgtcagcgat ctctattatc gttatgattt tttacgtcgt tattatgtgc aggcgggtcg
    12061 catggacaac cgcacactat ttaatgcaca aggcgggaac tttaccttta actttctgcc
    12121 actcggtgca attgatggga tgcgtatcgg gtcgacactg agctatttaa accaggcgca
    12181 aagccagcaa ggaaccccag taatggttct gctttcgcgc aattctcgtg ttgacgctta
    12241 tcgtaatgag caacttttgg gatcgtttta tctcaatagt ggttcgcaat ttattgatac
    12301 cagttccttt ccgcctggca gctacagcgt ggcattaaaa gtctacgaaa ataaccaact
    12361 cacccgcacc gagcttgtgc cgtttaccaa aaccggcggt ctgactgacg gaaatgcgca
    12421 atggttctta caggcaggta aaactacatc acaggtttct gatgatgaaa gttcagctta
    12481 tcagctagga gtacgcctgc cattacatcc gcaatatgag ctctacgcag ggctggcgaa
    12541 tgccgatgat gtgagtgctt tcgagttagg taataactgg acggcagatt taggcggggt
    12601 ggggaatctt gcaatcagcg ccagcgtgtt ccgtaacgat gacggcggca aaggtgatat
    12661 gcaacaggcc aactggagta atccgggatg gccgacgttg ggcttttatc ggaccaactc
    12721 tgacggcgat gcttgtacaa ccgacagcag agagagctat aacgccttaa gctgttatga
    12781 aagtatttcc gcgacggttt cacagaattt tgtcggctgg aatatgatgc tgggttatac
    12841 ccgcacacaa aataacactg atgatagttt gcgttgggat aaacagcaga gctttgaaaa
    12901 taactatctt cgccagacaa cagcgcaaag tatctctgaa actgtacaac ttagcgcttc
    12961 ccgcgctttt gtgatgcgtg actggatttt gagtacgtca gtcggtgttt tccatcgtaa
    13021 tgacaacggt ggcgataacg acgacaacgg cttgtactta tcgttttcgt tatctgacac
    13081 gccaacgatg gatagcaata acaacagcca ttcaacaaat gtgtctacgg attatcgtta
    13141 tagcgaacag gatggcgatc aaacgtcatg gcagttatcg catacctttt ataacgattc
    13201 attcagccat aaagaacttg gcgtaaccgt cggaggcctg aacaccgata ccataaacag
    13261 cgcggttaac gggcgttggg atgggcaata cggaaatgtc tacgctaccg tatctgatag
    13321 ttatgaccgt aagaatcatg atcatctttc ggcctttacg ggcacttata gctctacgct
    13381 ggctgtcagt cgctatggcg ttaatttggg agccagtggt acagacgatt tgctgggtgc
    13441 ggtactggtg gatgtgaaag tcttctctga acaggatgaa gatagtcagg atctgcaact
    13501 cgaagcgcgg gtggcaggca gccgaacttt gcagcttggt caaagtgaca gtgtgttgtt
    13561 cccttatcct ggatttcagt ctggttttgt tgaggttaac gacagtagcc agggcaatca
    13621 gcaggggaca acaaacatca ttaacggtgc agggaatcgt gaattaatgt tgttgcctgg
    13681 caagctgcgc tatcgcgaag tgtctgccag ctttaactac aactatatcg gtcgtttatt
    13741 actgccggca gcggtgaaaa aattcccgat tgtggggctg aacagcgcca tgttactggt
    13801 agctgaagat ggcggattta cacttgaaat taacggcagc gaaaaagagc tctatctgct
    13861 ttccgggcag caattcctta agtgtccact gagtgttgta aagaaacgcg ccagcattcg
    13921 ttacagcgga gatgttactt gtagtgtggt gacttattca caattaccgg agtcgattca
    13981 ggttcaggca cagttgaaac agcctaaatt acgtggaaac gttcagacgg cgcaaaggga
    14041 ggttgcacca tgagaaatcg attgattgcg gcgatattgg gcttgtttgg cacgctcact
    14101 ggcgttcagg cagctcctga cgtgaccagt gaaattacgt atgatttggc atctggtaga
    14161 gcggattatt acttctggaa ggatgaagcg tcggcaggaa ataatggata tatgtggtat
    14221 gaatgttcgt atcctgacct ccaacaaacc tgtacagcta atggaaatat atcgacagta
    14281 caaatctatt taactgaaca acgcagtggg atgcgttggc cggtaaaact caaaggattt
    14341 aaaacagcca ttgtaagtag cgatgaagcg ccgccaggat gcaagggggg caaagggctt
    14401 cagacgaatc ttaaggattc taatagatct tcatgtacag aagatggtca acattattat
    14461 atatacgata caaagtttct tacgctctac cttgagcaga cagagatgaa gaatttgccg
    14521 attggtggcg tctggaaggg gaaagttaaa ttacattcga acagcccggc ccaggactat
    14581 ttcgcaaata ttaccctgaa tacgctcgac cccaaccata ttgacgtgtt cttcccggag
    14641 ttcgcccacg ccacgccaag ggtgcagtta gacttgcatc caacaggaag cgttaacggc
    14701 agcaactacg cgcaagatct gaccatgttg gacatgtgcc tgtacgatgg ttttaacggt
    14761 aatgccatca gttatgaaat catgctcaaa gatgaagggc gaccagctgc agggcgcaga
    14821 gacggttact tctctatcta tcgtcaggga gggaccacca ccgacgaggg agaacgcatt
    14881 gattaccggg tcaaaatgta caacccggaa accggtgggc aaattgatgt gcgcaataat
    14941 gaaaatatgg tctggaacag cattaacctg aaacgtgtgc gtccggtggt actgcccggt
    15001 attcgctatg ccgtgatgtg tgtgccgaca cccttgactc tggcagtcga taaattcagc
    15061 gtgatggata aacaggccgg gtactacatg ggcaaattgt cagtaatctt tacgccttcc
    15121 ttgccaacca tcaattaacc ctgcaaaccg gacaaacgct tttttctata taaacgacga
    15181 agccgtctga taaagcggct tcgtcattac aacttaagtt aaacgttaat gttgcggtgc
    15241 gggatcgtta tgtgtttcct gcgccacctc aatcgccggt ttgttctgca acacggtcca
    15301 gataaccagc gcacctaaca ggtcgaacac tgccagaact gcgaacagcg ggctgaagcc
    15361 gatggtgtca gccagtgcac cgacaaccag cgcaaacagc gtacttgcca gccatgcgga
    15421 catcccggtt aaaccgttcg ccgtcgccac ttcgttacga ccgaacacgt cagaagagag
    15481 cgtaatcagc gcgccagaca gtgcctggtg ggcaaaacca ccgatacaca gcagcataat
    15541 tgcgacatac gggttggtga acagaccgat catacccggg ccaatcatca gcacagcacc
    15601 cagcgttacg accatcttac gggaaacgat caggttcaca ccaaaccaac gctggaacag
    15661 cggcggcagg taaccaccga ggatacaacc gaggtcagca aacagcatcg gcatccaggc
    15721 gaacatcgcg atctctttca ggttaaagcc gtaaacttta aacatgaaca gcgggatcca
    15781 cgcgttaaaa gtaccccagg ccggttctgc caggaaacgc ggcagcgcga taccccagaa
    15841 ctgacggtta cgcaggatct gaccaacgga cattttcttc gccgtgctca cctggtgctg
    15901 ggcttcctga ccattaataa tatagtcgcg ttcttcatcg gtcagatgct tctggtcgcg
    15961 tggatgttta tagaaaatca gccatgccat cgcccagata aagctcaatg caccggagat
    16021 gataaatgcc atctgccagc tgtgcattac gattgcccat accaccagcg gcggcgcaat
    16081 catcgcacca atcgaagaac ctacgttaaa gtaacctacc gcgatggaac gctctttcgc
    16141 cgggaaccat tcggagctgg ctttcagacc cgccgggatc atcgctgctt ccgcggcacc
    16201 gactgcacca cgagcaatcg ccaggccacc ccagctacca gccagcgcgg ttgcaccaca
    16261 gaacacggcc cacagcacag caaacattgc ataaccgatt ttcgtaccca gcacatccag
    16321 tacatagccc gctaccggtt gcatgaccgt ataagcagca gaataggctg cgatgatata
    16381 ggaatactgt tgggtggaga tgtttaactc ttccatcaga gttggcgcag ctgccgccac
    16441 agtgttacgc gtcaggtaac caagcacggt gccaagcgtc accagtgcga tcatatacca
    16501 acgtaaccct ttaattttac gcatgtaaaa cctcatcgtt ttgttatatc cgcaggaagc
    16561 gcggccatca tcccgccaga ggctgaagat gttagttacg accgggtaac cgaaaaaaaa
    16621 ccggcacgcc ccgaacgttg ccatgaaagt ttcgtgctaa ttcggcttcc gtaccggtta
    16681 acccgattca tctaacaaat gaatgaatcg gcaatttgca ttccgtcgac ttatgatttg
    16741 cgacggcaga aagataactt gtcatacaac tttaaaaggt gagagccatc acaaatgtgc
    16801 gaatatttgt agggacatta cctgacgaca gcgaggccag tactggcgcg gcctgcagag
    16861 agatttacca ctttgagagt aattttttta actacgttta ttgatctaac tcacgaaaat
    16921 atcttcggac tctggaaatt ggtgtgataa ctttgtcagc atcgcaccat aagcaagcta
    16981 gctcactcgt tgagaggaag acgaaaatga ctccgtttat gactgaagat ttcctgttag
    17041 ataccgaatt tgcccgccgt ctgtatcacg actacgcaaa agaccagccg attttcgatt
    17101 accattgcca tttgccgccg cagcagattg cggaagacta tcgttttaaa aacctgtatg
    17161 acatctggct gaaaggcgat cactacaaat ggcgcgctat gcgtaccaac ggtgtggccg
    17221 agcgtctgtg taccggtgat gcgtctgacc gtgaaaaatt tgacgcctgg gcggcgactg
    17281 ttccgcacac tatcggcaac ccgttatacc actggacgca cctcgaactg cgtcgtccgt
    17341 ttggtatcac tggcaaattg ctttctccgt caactgccga tgaaatctgg aacgaatgta
    17401 acgaactgct ggcgcaggat aacttctccg cgcgcggcat catgcagcag atgaacgtga
    17461 aaatggtcgg caccaccgat gacccgatcg attctctgga gcatcacgca gagatcgcca
    17521 aagacggctc tttcaccatc aaagtgctgc cgagctggcg tccggacaaa gccttcaaca
    17581 tcgaacaggc gacctttaac gactacatgg cgaagctggg cgaagtttcc gataccgaca
    17641 ttcgccgctt tgctgacctg caaactgcct tgaccaaacg tctggatcac tttgccgctc
    17701 acggctgtaa agtgtctgac cacgcgctgg acgtggtgat gtttgctgaa gcgaacgaag
    17761 cagaactgga cagcatcctc gcgcgtcgtc tggcaggtga aaccctgagc gagcacgaag
    17821 tggcacagtt caaaactgcg gtgctggtgt tccttggtgc cgaatatgca cgtcgcggct
    17881 gggtacagca gtaccacatt ggcgcgctgc gtaataacaa cctgcgtcag ttcaaactgc
    17941 tggggccgga tgtaggcttt gactccatca acgaccgtcc gatggcggaa gagttgtcta
    18001 agctgctgag caagcagaac gaagaaaacc tgctgccgaa aaccattctc tactgcctga
    18061 acccgcgcga taacgaagtg ctgggcacca tgatcggtaa cttccagggc gaaggcatgc
    18121 cgggcaaaat gcagttcggt tccggctggt ggtttaacga tcagaaagac ggtatggaac
    18181 gtcagatgac ccaactggcg cagctcggtt tgctgagccg ctttgtcggt atgctgactg
    18241 acagccgtag cttcctgtca tacacccgtc acgaatactt ccgccgcatt ctgtgccaga
    18301 tgatcggtcg ctgggtggaa gcgggcgaag caccggcgga catcaacctg ctgggcgaga
    18361 tggtgaaaaa tatttgcttt aacaatgcgc gtgactactt cgccattgaa ctgaactaag
    18421 gtctggggtt gatatgcaat acatcaagat ccatgcgctg gataacgtcg cggtcgcttt
    18481 agcagatttg gctgaaggca cagaagtcag tgtcgataac cagactgtta cgctgcgcca
    18541 ggatgttgct cgtggacata aatttgcgtt aacggatatc gcaaaagggg ccaacgtcat
    18601 caaatatggc ctgccgattg gttatgcatt ggcggatatt gcggcaggtg ttcacgttca
    18661 cgcccacaat acgcgcacga atctgagcga tctggatcag tatcgctatc aacctgattt
    18721 tcaggatctg cctgcgcaag cggcagatcg tgaagtgcag atctatcgtc gcgctaacgg
    18781 cgatgtcggg gtgcgtaatg agctgtggat cctgccaacc gtgggctgtg tcaacggcat
    18841 cgcgcggcaa atccagaacc gtttcctgaa agagaccaac aacgccgaag gtaccgacgg
    18901 cgtgttcctc ttcagccaca cctacggctg ctcacaactg ggcgacgatc acatcaacac
    18961 ccgcaccatg ctgcaaaaca tggtgcgcca cccgaacgcg ggcgcagtgc tggtgattgg
    19021 tctgggctgt gaaaacaacc aggttgccgc attccgtgaa acgttgggcg atatcgatcc
    19081 tgaacgcgtt catttcatga tctgccaaca gcaggatgat gagatcgaag ccgggatcga
    19141 gcatttgcat cagctgtata acgtgatgcg caacgataaa cgcgagccag gcaaactcag
    19201 cgaactgaag tttggtctgg agtgcggtgg ttctgacggt ctttctggta ttactgctaa
    19261 cccgatgctg gggcgtttct ctgactacgt gattgctaac ggcggtacta ccgtactgac
    19321 cgaagtgccg gagatgtttg gcgcagagca gttgctgatg gaccattgcc gcgacgaagc
    19381 aacgtttgaa aaactggtca ccatggtcaa cgacttcaaa cagtacttta ttgcccatga
    19441 ccagccgatc tatgaaaacc catcgccggg gaacaaagcg ggcggtatca ccacgctgga
    19501 agataaatcc cttggctgta cccagaaagc gggttccagc gtcgtggttg acgtgctgcg
    19561 ttacggcgag cgtctgaaaa cgccggggct gaacttgtta agtgcgccgg gtaacgatgc
    19621 cgtagcgacc agcgccctgg cgggtgcggg ctgccatatg gtgctgttca gtactggtcg
    19681 tggcacgcca tacggcggct ttgtaccgac ggtgaaaatc gctaccaaca gtgaactggc
    19741 ggcgaagaaa aaacactgga tcgactttga cgcgggtcag ctgatccacg gtaaagcgat
    19801 gccgcagttg ctggaagaat ttatcgacac catcgttgag tttgccaacg gtaaacaaac
    19861 ctgcaacgag cgtaacgact tccgcgaact ggcgatcttc aaaagcggcg taacgctata
    19921 aagtcgaaat gagttgttaa taaaaaacgg cgtttcataa tgtgaagcgc cgtttgcttt
    19981 tccatacctg ataaggattt atcatgaccg cgtattggct ggcccagggc gtgggtgtca
    20041 tcgcctttct gattggtatc acaacatttt tcaatcgtga cgaacgtcgc ttcaaaaagc
    20101 agctttcggt ctatagcgcc gttattggcg tacatttttt tcttctgggc acctatcccg
    20161 ctggtgccag tgccatcctt aatgccattc gtacattgat taccttacgc acgcgcagct
    20221 tatgggtaat ggcgattttt attgtgctga ctggcggaat tggcctcgcg aagttccatc
    20281 atcctgtcga actattgccg gttatcggta cgattgtcag tacctgggcg ctgttccgct
    20341 gtaaagggct caccatgcgc tgcgtgatgt ggttttcaac gtgttgctgg gtgattcaca
    20401 acttctgggc ggggtcgata ggcggcacaa tgattgaggg tagttttctg cttatgaatg
    20461 gcttgaatat cattcgtttc tggcggatgc agaaaagggg aattgatccg tttaaagtag
    20521 agaaaacccc tcccgccgta gacgaaaggg gttaaacaat taattacgca gggcgctatt
    20581 tgccagacga tcgtcttccg cctggcaagc tgccgcagtg aacagcacgt cagttgaaga
    20641 gttcagcgcg gtttcgcaag agtcctgcaa tacgccgatg ataaagccga cggcaaccac
    20701 ctgcatggcg atatcgttcg aaataccgaa catattacag gccagcggga tcagcagcag
    20761 agaacccccc gccacgccgg atgcgccaca ggcacacaga gaagccacca cgctcaacag
    20821 cagcgccgtg ggcagatcga ccgggatacc cagcgtatta accgcagcca gcgtcaacac
    20881 ggtaatggtg attgctgcgc ccgccatatt gatggtggca cccagcggaa tagaaacgga
    20941 ataggtatcg cgatccagat tcagcttttc acacagtgcc atattcaccg gaatgttcgc
    21001 cgcagagctg cgggtgaaga aggcatacac gccgctttcg cgcaggcaca gcagcaccag
    21061 cgggaacggg ttacgacgaa ttttccacca caccagcaat gggttaacca ccagcgccac
    21121 cagtaacata cagccaacca gcacgaccag cagttgcgcg tagccccaca gtgtggagaa
    21181 accggtggtt gccagggtag aagaaaccag accaaaaata ccaatcggtg cgaagcgaat
    21241 gaccagtttc accataaagg tgacggcatt cgacatatcg ttgaccaggt ttttggtggt
    21301 ctcgttaccg tgacgcagtg cgaaaccgag gccaattgcc cacaccagga tcccgatgta
    21361 gttacctttc agcagcgcgt cgatggggtt ggaaaccatg ctcattacca gcccgcgcat
    21421 cacttcgaca atgcctgacg gcggcgaaat atcacccgcg ctactggata aatgcagggt
    21481 agaagggaag gcaaagctga agactactgc ggccagagca gcagagaagg tacccagcag
    21541 atagaggaac aaaatagggc ggatattggt tttctgcccg tgctggtggt tagcaataga
    21601 tgccatcacc agcatcaaca ccaggatggg ggcaacggct ttcagtgcgc cgacgaacaa
    21661 agtacctaac agaccaacag cttccgccgc gggttttgag atccatgcca gaagaatccc
    21721 cagaacaagg ccgaccagga tttgttttac caggctgcca tgagccagac gccggaatag
    21781 ccccggtgaa cgttgcgtag tcatttttcg atcctttcat tgtgttgtcg cacatccctg
    21841 gtgcgttctg tcgacgcatc tttcaggatg taacaaagtg tttgcaaggt cgagtataag
    21901 gaaagttaca gcttcgggaa gcgaaaaatg ctggatttta aagagaggtc atattttgca
    21961 acttaattgt tacatttgtt tgacataaac agagggaagg actggctact aaggctgatt
    22021 agataacata tgggcaaaac tatttggaat atgagaacgg ttgcgctcct ttaatgtgtt
    22081 atgccatata aagtcactgg cgcagggagc gcgcaggggg cggccaatcg ccgccgcccc
    22141 ctgcaccccc aggctctggc gaacaaaatc gccgctgcgc ggttccctca gcttatccct
    22201 tgcggctacc gggtcgggcg caaggtaacg tccctgtaag atgcgccctc gccgcgcatc
    22261 catgcgcgtc gccccggcct ccagggaacg cttcggcgat tttgacgcca ccaacacccg
    22321 tgcggtcttt ttgtaaagag ctataagccg attacataat taatagctca cctttgtctg
    22381 gaatgagtta tctctagtaa tggcttaata tacagcggat cattctcccc gctgcttatc
    22441 atgccgataa ttcacccagg cgttgataat aaacgtcatc accagaatgc caaacaccac
    22501 gcccagcgag acggcgattg gaatatggta gaagtcgaca atcagcatct tgataccgat
    22561 aaacaccaga atcaccgcca ggccatattt gagcatcgag aaacgctctg ctacgcccgc
    22621 cagcaggaaa tacatcgcac gcaggccgag gatcgcaaac aggtttgagg tcagcacaat
    22681 gaacgggtca gtggtcacgg cgaagatagc cggaatgcta tccacggcga aaatcacgtc
    22741 gctcaattcc accagaatca gtaccagcat cagcggtgtg gcgtacagca atccattctt
    22801 acgcacaaag aaatgctcgt tgtcgatggt gtcggtcatg cgcaagtgac cgcgtagcca
    22861 gcgcaccagc ggcttgtcgc caatgcctga ttcatcttca tgggcgagcg ccatcttcac
    22921 gccggtaaac agcaggaagg caccgaagat atacaggatc cagtcgaact gtgaaatcag
    22981 ccagctgcct gtgaagatca tgatggtacg cagaacaatc gccccgagca cgccatacac
    23041 cagcacgcgg cgttgtaatg ccgccggaac agagaaatag ctgaacaaca tcagccagac
    23101 aaagacgtta tcgaccgcca gcgatttctc aatcagatag cccgtgagaa aggccagtgc
    23161 ctgtggatcg gcaacggcgc gaccttcagt ttgcaccaga taccaccaga aagcggcgtt
    23221 aaacagtaac gacagcgtca cccagaccag cgaccaggcc gcagcctgtt tcatggtcat
    23281 ggcatgtgcc ccacgacgcc cctgcaacaa caggtcgata gccagcataa tggcgacaac
    23341 aacagcgaat ccgccccata gcaacggcgt gccgacagta ttcatagaag ttccttacac
    23401 ataaaaaaac ggccaacgtc ggaagacgtc agccgctgct ttttatgcat agacctcgcc
    23461 ttccggcaag gtctcactta caacaaaaaa ggaatacgtc tgcgtattcc tttcgggttg
    23521 cccggtgacc ggatgtggtt tttcacacat cgtaatgacg atcgaccggc aatgaagtta
    23581 ctcccctttg caggtaacaa agtatgacag accattcagc cagtcaatgg tctgttacat
    23641 accttttaca ctgttttact ttggcaatta tagttttacg ctatctgccg gaaaaatcac
    23701 cccagtctgg cggcggatct cggtcagcag tttggcggtg atgcgactga ccgccagccc
    23761 cggatgatcc accagatgct catccaccag ggtagcgaac agctctgctt cgtagagcat
    23821 ggtattaata tgctgcggct gggtgagatc ctgcatttgg ctgccacgcg gcacgaagca
    23881 cactttctgg cattcagaca gtttttcaat caccagcgat cctgcttcgc cctgaatctc
    23941 gctcgccagg acagaatcac tgacttttga gtgctgcaag gtgacgctga aatcaccgta
    24001 atccatcacc accacgccgt gggcatctac accgcttgcc agcaaactgg cggttgcctg
    24061 cacgcttttc ggctcaccaa ataacgccac cgccgacgcc aggcagtaaa agccgatatc
    24121 cataattgaa ccgttagaga atgacggatt aaaggtgttg ggattttcac cgtccaggta
    24181 acgttgatag cgcgaggaat actggcaata gttgaaaaag actttacgca atttgccgac
    24241 tttcggcagc gcctggcgca gcaaatgaaa gttcggcagg caggcggttt taaatgcctc
    24301 aaacagcacc acctgatttt cccgcgcaca ggcaatggcg gcatccactt ccgccagatt
    24361 cgacgccagt ggtttctcgc aaatcacatg aattttatgg ctaaggaaaa gttgtgtctg
    24421 ggaaaagtgc agggcattcg ggctggcaat atacaccgcg tcaatggcat cgctttccgc
    24481 catcgcttcc agcgaggtaa acagatgctc gacagaaaaa tcattggcga agtgctgagc
    24541 ctgctcaagg ctgcgggaat atacggcggt taacttgtat ttaccgctct catgggcggc
    24601 ctcgacgaac tggcgagtga tccagttcgt accaatcaca gcgaaacgta tcataacgcg
    24661 tggatactcc ataaagggat tctgtcgtca atgtagcacg cctttttcat cttcgacgtg
    24721 cgcagatacg cattattctt ttgccggaca gccatgctca ctaaacggcc caatcatgac
    24781 cgatggcccg agatatttcg gctggcgatg gaggattttg gcgtcaatca cggcgctgac
    24841 acgagcgagt ttctcccgca gttgagtata aaaaccgtgc ttcatattga ggtcaggcgc
    24901 gttaaaaccg atagcgtcga tgctgtactg ttttgccagc cagatggcgc gttggttatg
    24961 aaattcttgc gagataatgg tgatatggtt ttcaccaaaa acctttttgg cacgcactac
    25021 cgaatccagc gttgagaatc cggcatagtc gcagaagatc actttcgccg gtacgccttt
    25081 agcgatcaaa gccttctgca tacctgatgc ttcatcgtaa tttttacgtc cgttatcgcc
    25141 acttaccagt agccatttca cttttccggc gtggtacaac gctgctgcgg tatcaatacg
    25201 tcgggtaaag tagcgattac ccggcctggc ccccagcaat aagcccacgt tacgctccgg
    25261 aactgcgttg acatcgctcc aggtcagctg cttgctggcg ttgaccataa cgcgatcggc
    25321 aataaaaatg gtcgctcctg ctataagcaa caacaaacag ccaatcttaa gagtccggcg
    25381 agaaaaacaa atacggagaa gaaggcgggg ctgcatggtc gttatcctta acgaaagggg
    25441 ggaggtcgat tttaggcgct aaagaaagaa aagataacgc ctgacatcat tttcagattt
    25501 tgtcctgccc aagcgacaac tgcgttagcc acagccgggt gtcgaactca agctggtggt
    25561 actgcggctc catatggcag cacaactggt aaaacgcttt gttatgctct ttctccttca
    25621 ggtgcgccag ttcgtgcacc acgatcatac gtaaaaacgg ttccggcgcg ttgcgaaaca
    25681 cagtagcgac gcggatctct gcttttgctt tcagcttgcc gccctgcaca cgagaaacag
    25741 cggtatgtaa cccgagcgcg ttttttagca catggatctt attgtcatac atcactttat
    25801 tgatcggcgg ggcattacgc agaaactgat ttttcagatc ctgagtatat tgccagaggg
    25861 ctttatcggt ggcgtagtcg tgtgttcccg gatagcgttt tgccagcaca tcgcccagac
    25921 gctgttcgtt aatcagcgtt cgcacctggg aaagtagctg ctcgggataa ccctgaagat
    25981 aagtaagatt gttcataacc gcccacgaaa aagagaaaaa gggtatactc acgcaccctt
    26041 ttcaggggca acgtcgaaat tttatcattc aggagggccg atgagccact tagacaacgg
    26101 tttccgttca ctgacactac aacgttttcc ggcgacggat gacgttaacc cgctacaggc
    26161 gtgggaagcg gcggatgaat atttgctgca acagttggac gacacagaaa tccgcggccc
    26221 ggtgttgatc ctgaatgatg cctttggtgc gttaagctgc gcgctggcgg aacataagcc
    26281 gtacagcatt ggcgactcat acatcagtga actggcgacg cgcgagaatt tacgcctcaa
    26341 cgggattgat gagtcgagcg tgaagtttct cgacagcacc gccgactacc cgcaacagcc
    26401 gggtgtggtg ctgatcaaag tgccgaaaac actggcattg ctggaacagc aactgcgtgc
    26461 gctgcgcaaa gtggtcacgt cggatacacg tattattgcc ggtgccaaag cccgtgacat
    26521 tcacacttcc acgctggagc tgttcgagaa agtgctcggc ccgaccacca ctacgctggc
    26581 atggaaaaaa gcgcgcctga ttaattgcac tttcaatgaa ccgccgctgg ccgatgcgcc
    26641 gcagaccgtt agctggaagc tggaaggtac tgactggact atccacaacc atgcgaatgt
    26701 cttctcccgc accgggctgg atattggcgc acgcttcttt atgcaacatc tgccagagaa
    26761 tctcgaaggt gagattgtcg atctcggctg tggtaacggc gttattggcc tgacgctgct
    26821 tgataaaaac ccgcaggcga aagtggtgtt tgtcgatgaa tcgccgatgg cggtcgcttc
    26881 cagccgaatg aatgttgaaa ccaacatgcc agaggcgttg gatcgctgcg agtttatgat
    26941 caacaacgcg ctctccggag tggagccttt ccgctttaat gctgtgctct gcaacccgcc
    27001 gtttcatcag caacatgcgc tgaccgataa cgtcgcctgg gagatgttcc accacgcccg
    27061 ccgctgcctg aaaatcaacg gcgaactgta tatcgttgcc aaccgtcacc tggattactt
    27121 ccataaactg aagaagattt tcggcaactg caccaccatc gccacgaata ataaatttgt
    27181 ggtgctgaaa acagtgaagc tggggcgtcg tcggtaagat tgggagttac ctgccggatg
    27241 cggcgttaac gccttatccg gcctacgttc agcacgttac tcccaggccg gataagacgc
    27301 ggcaagcgtc gcatccggca atgtctgccc agttctggaa tcgcgcttcc ataaaacaaa
    27361 aaatccctgt caccatcctc tacattctct tgtttagcgt tgttcaacgt ttatgctaca
    27421 tacgtgaagt tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg
    27481 ggaaaaaatg attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgcggtggc
    27541 accttttctg gcgggtattc agaacgagga acaatacacc caggcgctgg aactggtaga
    27601 tcatctgctg ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac
    27661 cgcgtgggaa gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc
    27721 tggcggtata gccgtgattc gtacccttat ggatcaatat ggtttaaccc tttccgatct
    27781 gccggaaatt ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac
    27841 gctggaacac gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga
    27901 ttaacgcgac gtgccggatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag
    27961 cgccagccgt gttccctggg caattgcccg tcgtgcgtcc agctccatag ccacatcgca
    28021 gccgccaatt aaatgcacag ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt
    28081 tggctcttgc cctgcgcaga tcaccacatt gtccactgct aatacctgcg tttcgccgtt
    28141 gatcaccaca tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt
    28201 cacaccccgc gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg
    28261 tcccggtttg ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg
    28321 cattccctgc gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt
    28381 acagaacctg gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt
    28441 atcaaaacca atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc
    28501 gcgcagtaca tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac
    28561 gcgcggcacg atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt
    28621 cacggtgtga ttgagtttca gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg
    28681 cagcgtttcg taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc
    28741 gatctcgctg tgcgcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc
    28801 aaacgccagc ccagcaggtc cagcgccgac caccgccaga ttttttttct gcacggcggg
    28861 aaggattggc attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt
    28921 gccaacgaag atctgatcga ggcaggcctg gttgcagcca atacaagtgt tgatctcatc
    28981 ggctcgccct gattgcgctt ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat
    29041 cgatactata tcggcatcgc cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat
    29101 ccggttggtg gttaccagcg gcagcgagac gtggcctttc agtttgcgcg tgacccagct
    29161 aaatgcgccg cgcggcacgg gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat
    29221 tccggtgttg ataatggtcg cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc
    29281 ggcaaaagtg ccgccgcctt cgaccaggtc gagcatcgac aaccggtaaa taataatgaa
    29341 gtcgttgccg acgcgttcgc gcaccgtacg cactacttct acggcaaatc gcatccggtt
    29401 gcggtaatcg ccgccccact ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt
    29461 gatcaaatac ccttcagagc ccatcacttc gacgccatcg tatccggcct cccgcgccag
    29521 ttgcgcgcag cgggcgaaat cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg
    29581 aggaacgaaa cggttgatgg gggcctgcaa tgcggacggt gcgaccagat gcggttggta
    29641 gctgtagcgc ccggtatgca aaatttgcag ggcgattttg ccgccttcct gatgtacggc
    29701 ttcggtaatg gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgccaccttc
    29761 catgccaacg cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg
    29821 acgggcgcgt tcggcataaa acgctgccag ccgttcagca ccgtccgggt attcctccag
    29881 cccggtgtgc attgaaccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag
    29941 cggggcgaac agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt
    30001 ggtcggatga gttctaattt agccgtcgcc ggagaaaagg gaaaagggga gtgcggggtt
    30061 tgtgatggga ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt
    30121 ttaatccggc ctgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc
    30181 agtggcgcgg tgcagatgcc ggatgcgacg cttgtcgcgt cttatccgac ctacgggaac
    30241 gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg tgcagttgcc
    30301 ggatgcaacg cttgccgcgt tttatccggc caggaggatt gcacatgtag ggcggataag
    30361 gcgtttacgc cgcatccgcc agcagttgca aattattgct tacggaaaaa atcgttatac
    30421 aacatataca aatgcgcggc actccaggag aaatttggtg cgccttgctg tgcgccagtc
    30481 agcgggttgt aattctcctg aattgggcca tcggcggtta atcctttggc gtgacggaag
    30541 aacgtatccg ccagtttcag ggcatcatcg cgataaccgt aacgctccat ccctttcaga
    30601 ccaaaccaga actgatccac ccatacgcgc ccgcgccagt agatatcagc gccaaaagct
    30661 ggattggtta acgccgccgt tcccagcggg acaaaggtgt tgaactcttt aggatcgagc
    30721 atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg caccgttaaa cagcggcgac
    30781 cagccttccg gccctttacc gcgctcaaca atcggtttgc ccgcgcagcc gttcgccagt
    30841 ggtttatctt caatacgtac gtcatagtag aactgggtag tcgggtcgaa catacaggtg
    30901 ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc gtttggcctc ttctggctta
    30961 ccgaggatgg ttgccatctc cgccagataa tggttatcgc tgtacatata gctggcctga
    31021 tccaccgact cctgcaatag cgagtagccc agcaacgttc cgtcctgact gcggttttcg
    31081 gcgaatttca ccgtccagtc gctacgtttg ccgccattag cgacatattt atccaactgt
    31141 tctttgtcga taaaaccaaa gacggcggca tcatcacgcc ccgattccca cgacgcagcg
    31201 acctgtgccg gaatttccag actgtcgtac tggcctttct ccaccacgcg ggcgtagttg
    31261 ttcagcccag actgcgtctc ttctttgttg cctttcttca ccgtaaacag catctcgccg
    31321 ctctcagtgt tgtgggcttt gtcgcgggtc gcgccatatt ccggcacgcc gttgccgttg
    31381 tgatcgcggt tacgtaacca ccagtcgtga taggccacca gtttcgggta catctctgcc
    31441 aaccaggctt tatcctgggt gacgttgtac acttccatca ccgaccaggc ggcaaggctg
    31501 ggtttggtat tacgttcgtt ccagttgccg ccatcgccac cacgctcggg gctaaggttc
    31561 cacgctatca ggtcggggac aaagcccaca tcctgcggac gcacgctatc gccaggctgg
    31621 atctgccagg agaagaccgc gcggatattc tctttggcga tgtccggatt gaaatgcgcc
    31681 atcgcaaacg cctgcttcca ggtatcccac ggccaggtct gattgccgga gaaccagcgc
    31741 ccggtcaccg acggtgtaac ggtgttaaat ttcaccgcac cgcccggtga gcgccagtta
    31801 ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga
    31861 ttggttaacc ctttcttcag gtattcttcc cagcgttgct gcgaggcggt gagataaaac
    31921 gccggacgcg ccagaatatc gcggatctgc atttgctctt tgctaacttc ctgagcggtc
    31981 agcagatggg aataggtggt gtagagcgtg gtcgaaccgt tgatatgcgc cttactggta
    32041 aagcgattac cgttgatttc agtctgcacc ggcagggatt tatgcacctg atattccgat
    32101 tcgccggagg tcagcagatc ccaggtggcg cgcactttgc caaaggtgac tttcaggcca
    32161 tcacgggtgg cgctgatttt gcgctgatag tcagggtatt cgccagcaat ggttttatcg
    32221 gaaagcggtt tcccttcttt cgcttccagt ttttccagca gttcgccgtc ccacaccaga
    32281 tccagcggtt tattgctggt gattttggtt tccagtagtg acgtgcgcgg cgtggcgaag
    32341 cgcagagtca tttcgacttg cacatctttt gctgtcagtt tttgcaccag cgcaccggga
    32401 atactgtatg cctccagcgt aaagtcgact ttcttgccgt cctgccagac ggtcaggcgg
    32461 tcgaaattgc tggccataaa gttgatgtac tcttccgtca gcagcgcaac gcccggaaag
    32521 ccgcccatgg tgttagggcc gtctggcaac agatgaccat gccaggctcc gagatcgaaa
    32581 aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg cggcgcgcca
    32641 gtacggttaa tcacgttttt ataattgtcg gcgttagcgg catgggcgga aaaacttatc
    32701 agcagagcgc aggttactgg cgttaaaata gtttttattt tcatgaaatt acagtcctta
    32761 ttaccagtag aaatattgca tcaggaagat ttggtcgctg ggatcgtgag aggcattttt
    32821 catgtagaag cggctatcaa aagccgtggc gaaacgtccg ccgccgtatt cgtaccagat
    32881 accaaactgc atgaaagagg tggtttcatc gtcagcgtgg tcagggcgat gcttggcgta
    32941 ggtgtaagcg gtggagagat aaacgccctt taacgcttca tacatggcgc tcaccatcat
    33001 cccactttca cttcccggta cgtccagccc gtcggcgcgt ccggtatggt gccaggcgcg
    33061 agcggcaaag ttaggagtta gcaggcccgt gagatagagc tgtcctgtgc cgtcggtgat
    33121 ttccagaccg ttcatccagg tgatgccttc ggcaaggtca tactggatgt aaccgttcat
    33181 cattgccgga taggtgtatt tgtcgtcata gcgatcgtac ttgccaaaat gtagccaggc
    33241 tttgctttcg tcatgatgtc cggcaggtgt ggcggtgacg ctataacgaa agttgccgct
    33301 taagttctgc accttcagcg cggtcataat gtcgcgggta ttgggaatga cgtagcccag
    33361 atcgggggtg aagtcgcccc accattgcag gtcatccagc gaggaatcct gacgcagact
    33421 gagcatgatt tcggtgccat cattggtacg ataaccgcca tagaaacggt taattccgcc
    33481 ctcgaaacca ccccagctat ggtctggcac ccaggcattg ccgtcgtggt cggcctgcac
    33541 ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg tacttcatct ccaggccacc
    33601 ttcaatatag gtaccgtcgc tgtatttatg tttattctca ccctctaaat ccatatatcc
    33661 accaacccct aattcgccgt aaaaacgaaa tgaaggagaa gatgtcgagg taattgcagg
    33721 tgtttttgtg tcgtcatgtt ctgccggacg tgcttcttct cctgccgcag aaaaggcgta
    33781 gcaaaacggc agagcaagta atgctctgca tggtgcagtg attaatttca ttgtgttaat
    33841 ccatgagcta aagaaaaagt acagcctttt atcgttgtaa ttataaaaat ataaaggcag
    33901 aaactatttt ccctctcctc atccaggaag agggaaaatg cctgctcatt ttttattatt
    33961 ctgcttaatc aacattgctg gctggcgtcg cttcaatatg gcgctcttca gccgttaatc
    34021 cctttatata tttactgtat ttccaccacg caaagccgag gaagataaca ataccgccga
    34081 cgttataaaa aatgatggtc agaatattcg cgccagtcgg gaatgtcgaa gcgacaaacc
    34141 ctacggcaaa tatcgcaatc agcattgaaa caacgataat accggtgcgg cgggagccca
    34201 tacggaaatc gcgtggcagg tgatctaatt tggcgcgtaa attcaggtaa gccagcatga
    34261 tgaataacgg cggaagcatg gacgctgcgg cggtcatatt aataatagta ttcatcagat
    34321 cctgcacggt attggaaccg agcatcggga taatcatcag cgggatgacg atcaggaact
    34381 ggatccacgc tgcgcgcgcc ggaacgccgt tttcgttcag ttcgacggtt ttcttaccaa
    34441 agatgccttc cgggatttcg gagaagaaaa ttttcaccgg cgttgcggtc cacatcagca
    34501 gggaaccgaa catcgcggta aaggacacca gaccgacaaa gcgattcatc aacgcttccg
    34561 gtagaccaaa atacgccgcc atgccgtgga atacctgcac cgatccgccg gtaaatttca
    34621 actctttgct gctgacgaag acgttaatca gcaccgagga gacggaatac agtacgccga
    34681 taaaaatccc ggcgaggatg atcactttaa cgaacgattt cgaaccgcct ttgacgtcgt
    34741 taacgtacac agcgacggac tccgcgccac ctgcggccat aaagatccag gtggtaacgc
    34801 cgaggaacgc ccagttgaag ttcgggatca tcgagtcaac ggtgatgggg tcagcaggct
    34861 gtacgccgcc aaccagcgcc gtacctgcca gtaaaatgta ggagagcgtt aacagcagca
    34921 tcagcgttga agtgacggag gtaattggcc ccaacatttt cgccccgttg gtggaaaccc
    34981 aggtggagaa ggcgaacagc accatactga taatggtggt ggcaaccggc gtcataatat
    35041 attcgtagcc gaggaaggca tacgaagcat aagcaataac gcgcggcaac agtgaggtga
    35101 aaaagaacag gtttacgaac cagtaggtat aggcagtaat aaatgcccaa cgtccgccca
    35161 gcgaactttt tacccacgcg tagacaccgg cttctgagtt tttatttaac gaaacaaatt
    35221 ctgcgatgat cagacagaag ggaataaaat aaaaaatcgt cgcgaggaaa aacatcggtg
    35281 ccgaggccag tccaagctca atattattat tgataacgtt gttaaagctg taaacggcgg
    35341 caaaagtcag cgagagcaag ccgaatttgc cgattgtatt acgtttggta tcagacataa
    35401 cacatctccg aaaatgtgta gggtagcggt ggcggcaaac tccttttgcc gccgtagttg
    35461 ttatttgtta tggaaataac catcttcgat ggtgactttg agaaccactt ttttgaccgc
    35521 gttattgcag ataaaccgat acgcttcatg gatatcgcaa ataacgattt gcccttcgtg
    35581 gacctcaacg gtttctccgc agccttttaa atattcacgg tcagtttcat cacgataata
    35641 ttccactacc tgtaatgtct ctttcggcgc atattcaatt ttttgctgcc cttgcaggta
    35701 gtaatgcact tcaaaatagc gtcgatgccc ggtaaatagc gcatcggtcg cggagtctgt
    35761 ctccacgcgg taagtcaatg agtcaccgat ggagtgggcg acgccaggct gaatgttgtc
    35821 gatattttca atcgcttcaa cgcagcgttg ccacttcttg ccagaggcgt aaatctggcg
    35881 gaactgttct aagttatcga tgatcctcat tgcttattct cgctgtgcaa attcgtggaa
    35941 aagaaccctg cgccgaactc atacgacgcc aggctttgcg cggtagcttc tccgccagaa
    36001 accggcagca acgtaaagcc gtagctgaag tcacggaacc agacgcgcca ggagtccagc
    36061 acctcgctgc cccaggagtt ggagccgagg ccaagcagct ggtgatcgag gttcagggtg
    36121 atgtcatcac tgcgctgcag ctcgttacag tgctgggcag cgtggatgtt ttcctgggta
    36181 tagtgccagg cgctgaagtt aattgggcgc tgcggaacca ccagcaaacc gttgccgtgg
    36241 cggttagtca gtgccgtcca gcggacatgc tgacggttgc cgttgttctg cgggaaggga
    36301 tagttctcga acatggcatc gacggtgctg cgccagatat cgatgatgtt agcctgctgg
    36361 ctgtcggcgt agttttcgcc cggtccacga ccgtaatacg ccacctgatc gtattcgccg
    36421 ttaatcccca tggtgaaacc gatgcacgga atgatgtgcg gatagtcgcc gtaacgctcg
    36481 ccggaaagcg ccacgttaac ctggccatcg gcagcgatgc gccagatgta ggtgcagcgc
    36541 atcccgaagt caaacaccgg cggggcaata actgtgcggc tgatgatcaa cacttcacca
    36601 tcgctctgtt ctacggcaaa gtcgcgcaga tgttcctgca tgatctgcaa atgattcggt
    36661 tgccacagcc cttcgtactc ctgcttgtgg ttgtcgatca ttggcttgaa gaagttgatc
    36721 tttggctcgc gggtcagcag cgactcgcca ttcacctgcc aggatgtcgg tttgccactc
    36781 atttttgaga aggtgatcgc gaagttgtag ccgcgaacgg tgcagctcaa acgatcgtct
    36841 tccagcgtca gcggacgcgc attatttggt gcgaaaggca ctggttgcgc ggtgttttcc
    36901 ttcagcggga actgataagt ggcgatagaa tgcccggctt cgctgtagcg ggtgcgggaa
    36961 tctttggtca ccgtaatgtt gaggaacgct tcgcgggcgt ccagctgcgg cagcgtgatc
    37021 tgcaaggggg cttcgctgtt cggcgcaacg tcgcgcagtt taatctgctg cgtcgcgagc
    37081 gtttcacctt cgacgcgcac ctctgcgtgc agggtgtagt catcaagcgt ggtaaaccac
    37141 agtttatttt cgactttcag ctcgccgtga gtcagatcca gcgcgtggat ttttaccggc
    37201 gcgataacct gtttgtactc tttcaggccc ggtcccggcg tctgatcgga atagatcaaa
    37261 ccatcaagac agaagttata gttgttggga tagtcgccgt agtcgccgcc gaatttatac
    37321 cagacgttgc cgttgtcatc ctgcgcctgg atcccgtggt cgcaccattc ccagacataa
    37381 tgtccctgaa tgcaatcgtg cttatagaag acgttctggt actccgtcag cccgcctggt
    37441 ccgttcccca tcgcatgagc atattcacag atgatgcgcg gcttcggatg cgggtattca
    37501 ccaaactcat tcatcagcgg cacgcgggtg tacatggtgg aaataatatc gaccacttca
    37561 gcatcgcgat cttcttcgta atgcaccagt cgcgtgtcat ccagcgcctt cgctgcgtgg
    37621 tacatcgcgc ggatgttaca gccatagccg gattcattgc ccagcgacca gatgatgatc
    37681 gacggatggt ttttctgcgc gtgaatatgg cgaacaatgc gctcgacgta gactttttcc
    37741 cactgcggat cgtcggtaat acggctgata tcgccgacat tagcaaagcc gtgcgattcg
    37801 acgtcggttt ccgccatcac aaacaagccg tagatatcac acagttcgta aaaacgcgga
    37861 tcgttcgggt agtgagcggt acgcacggag ttgatattgt gctgcttcat caactggaga
    37921 tctttctcga cgcgatccat tccaacggcg cggcctttgc gatgatcgtt gtcgtgacgg
    37981 ttgacgccgt gcagcatcac ataacggtta ttgatccaga acagaccgtc gcgcactttg
    38041 atatcacgga agccaacgcg ttgtggcacc acttccagaa cgttgccgtt ggcgtctttc
    38101 agcgtcatga ccagatggta aagataaggg gattctgctg accattgctg cggctgttcg
    38161 acagtaaaag caaagctggc gctggtcagt ttttcaattg ccaaatgatc aatggcgctg
    38221 ctgtgcacca cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac gacaggggag
    38281 gcggcgagat tttccagcac cacttcgcag gaaagcgtgg catcgcaata ggcttcgtca
    38341 aagtcggtac gcacagtgaa atcgttaata tgcgttaggt gttttccgac cagataaaca
    38401 tcgcggaaga tccccgctga ccaccacata tcctggtctt ccacgtaggt agagtccgcc
    38461 cactgcatca cgcgcacaca caacaggttg tcgccggttt taaccatcgc gctgatgtca
    38521 aactctgcgg tcaggcgact gcccttgctg aaacccacat actgaccgtt aacatagact
    38581 tcaaaatagg tttcgacgcc gtcaaattta atcagcgtct gtttaccctg ccagccgtcg
    38641 ctgagggtga aaatacgttg ataggcaccg gttgggttat cgctggggac aaacggcaca
    38701 tcgatgggga acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc ttccatttgc
    38761 cacatggcgg ggacggtaat atgcccccag tcagccatta actcagaggt gaaggcttct
    38821 ggtacttgca gcggatggtc aaaaaagtgg aaattccact gaccgcttaa gggcagaaac
    38881 aggctgctgg tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata tgaaaaaaag
    38941 tacgcacgcg gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca gcgattcata
    39001 gggcctcccg gtagagagaa atcggaacca ttcatggtga tggtagtttg tgtaattgct
    39061 ggggagttta gtaaaatttt tactaaaaag tagcaggtaa atgcatttac tttaactgga
    39121 tcacgaaaat aaacggcagc actgtccggc aaaaacgatt gccggacagt gtgtaagggg
    39181 atttaacggg tcgtgccgcg cagttttaat ttgctgggaa cgaagactaa cagcggcagc
    39241 gcgcgaccat cgcgggcttt ttcatacacc aggttaacgc cctgacttcc catcatctcg
    39301 gaatggatgc gcacggtgga gagcggcgga aaggtaaatc gcgcggtggg gatatcgtta
    39361 acgctgataa gcgaaatatc ctgtgggatg ttcaggcctc gttcatgaat tgcccgcagt
    39421 acgccgatag caatggaatc ggaagcaaca aacagtgcct tcggatagtc ttcccgcgcc
    39481 agcatttgtt ttgccagttc ataacccgac gaactggaaa aaccgccgcg ccagatatcc
    39541 tcttcgcgta ccacttgttt cagtcggcca tattccgcaa aggcgacctc acgaatatcc
    39601 gccttgccag gctcatcttc accgccaata aaaccaatac gattaacgcc ctggttgata
    39661 tagaagtcga tgatttcttt actgatgcgt gccagatcga tatccaccgc atcgtaaccg
    39721 ctgccgggtt cgtgaaagtc gataaaacag atattgtcgg tcaacgcaca ggcggcggcg
    39781 cgcagggcgg gcgtgggttt gccgacaatt aaaataccgg tgacgttttt aatgtctggt
    39841 aatccgctgt gttcataaca gttggtgagc tcaatgccca gcttttcgca ctgagtttca
    39901 atgccgtggc ggatcgccag atagtaagga tcgttgatct ccagctcctg ttggtagctg
    39961 tagatagcca gaatatggtg ttggttgact gcacctgtct ggagtttacg ggcactactg
    40021 gtcttgtact ccagcttttc ggcgatctcg agaatgcgat gtttcgtctc ttctttcaca
    40081 ttcaatgtcg gatcgtcatt taagaccctg gatactgtcg ccagggatac gccagcttcg
    40141 attgcgatgt cttttagtgt tgccatgttt acctttttta ctttccctga tgtgcgcccc
    40201 cattgtagag gaagtgctta tcgggtcacc tgttttcagt aaataaagcg cgtttctttg
    40261 acgctgtaac ggtaacacag cggctcacga atgtgatcac gcctgcattc tccattttta
    40321 gcctacagga ttgagaaaaa cttttgctat agtttagtaa aattttaccc gaagggagga
    40381 aacaatggaa accgtggctt acgctgattt tgcacgtctg gaaatgcgcg tcggaaagat
    40441 tgtggaagtg aaacgccatg aaaacgccga caagctgtac atcgtacagg ttgatgtggg
    40501 ggaaaaaaca ctgcaaaccg tgaccagcct ggtgccgtac tacagcgaag aagagctgat
    40561 ggggaaaacg gtggtggtct tatgcaatct gcaaaaagcg aaaatgcgtg gtgaaacgtc
    40621 ggaatgcatg ctgttgtgtg cggaaaccga tgacggcagt gaaagcgtgt tgttaacgcc
    40681 ggagcggatg atgcctgcgg gcgtacgcgt cgtataaaaa gatcggatgg cgacgtcgta
    40741 tcgccatccg atttgatatt acgcttcttc gacacttact cgcatggccg ccagcgcctt
    40801 acgcgccgct ttgatcacca gttcacactg ttcaatggtc agtgtcagtg gcggttcaat
    40861 gcggatcgtt ttggcgttat tgagcgttcc ggccaccagt acgcgctggc ggaacatctc
    40921 gctggcaaag ttatagccga tttcgttatc aacaaactca attgccatca acatcccttt
    40981 accacgcgct tcctgtacca gatcgggata ttcccgcgcc agttgacgga aaccgtccag
    41041 caacatatcg cctttttgct cagcctgagc cggtaagttc tgctccagca acacattgat
    41101 ggtcgccagc gccgccgcac aggccagcgg gttgccgcca aaggtggtgg tatgcaggaa
    41161 tgggttgtcg aacagaactg aaaacacctc ttcagtggcg atggtcgcgc caatcggcat
    41221 cacgccgccg ccgagcgctt tggcaaggca gaggatatcc ggctgtacgt tctcatgctc
    41281 gcaggcgaac atcttgcccg tgcgccccat gcccgtttgt acttcatcga ggatcatcag
    41341 tgcgccgaac tcatcgcata gcttacgtac ggcggtgaga tagcccggcg gcggcagaat
    41401 tacgccacct tcaccctgaa tcggttcgag gatcaccgca gccacatcat caccggtttt
    41461 tttgcactcg ttaagagccg tgcgcatggc ttcgatattg ccaaacggca catgacggaa
    41521 gcccggcagc agtggcataa acggtttgcg gaaggtcgat ttcgccgtgg ccgacagcgc
    41581 gccaagtgat ttaccgtgga acgcgccgct ggtggcaata aaagtaaact tgccgcgcgg
    41641 tgactggtaa gctttcgcca gcttcagcgc tgcttcaacg gactcggtgc cgctattaca
    41701 gaagaagctg tatttcagtt taccgggcgt tagcgcagca agggttttcg ccaacatcgc
    41761 ccgtaacgga tcgagcagct cctggctgtg cagcggttgt ttcgcaagtt gattctgtac
    41821 ggcggaaacc acaactggat tacggtgccc cacgttgaaa attccaaaac ctcccaggca
    41881 gtcgataaac tcctgtccct gggtgtcgac aagcgtattt aaacctcccg cttgccactc
    41941 tacggctccg taatccccgc cggcggtaac agatttgcga tactctaaaa accccggatt
    42001 gacatgctct ttgaagtatt caatcacctc tcggttaagt gctttcatct cctcatgatc
    42061 cagcgttcgc ttttcaatga gattcagggc gtgggcgctg cacgctaaag ccgatgcgct
    42121 cgaaggtaac ctgttcaaaa tatgctccgg aggctcgcgt atcacatgat accgatttaa
    42181 gtattgcagg gattgcgcca ctccggctgc gatcgcgaaa atcgggataa acacaaattc
    42241 ggttatcatt tgtgcaatat ttaatgctat ttgttaacat ttgttgttat tttgcacggt
    42301 ttttgtcggg tatggatttg atgattgctg cgcaaatgca cgttttcagg gcaataattg
    42361 cctgattttt gggcaagata attaacgttt tcgtatagta acagagtgaa agtattttaa
    42421 atcaaagaat tagatttgtg ctttaatcgc aaattgcgat ctaaatcaaa ttaatcggtt
    42481 aaagataacc gcagcggggc cgacataaac tctgacaaga agttaacaac catataacct
    42541 gcacaggacg cgaacatgtc ttctcatccg tatgtcacct agcaaaatac cccgctggcg
    42601 gacgatacca ctctgatgtc cactaccgat ctgcaaagct atatcactca tgctaatgac
    42661 acttttgtgc aggtgagcgg ctttaccttg caagagttac aagggcagcc gcacaacatg
    42721 gtgcgtcacc cggatatgcc aaaagcggcg tttgcggata tgtggttcac cctgaaaaaa
    42781 ggggagccct ggagcggcat cgtgaaaaat cgccgcaaaa atggcgacca ttattgggtg
    42841 cgggccaatg cggtaccgat ggtgcgcgag ggaaaaatca gtggctatat gtcgattcgt
    42901 acccgggcga cggatgaaga gattgcggcg gtggagccgc tgtacaaagc gctgaacgcc
    42961 ggacgtacca gtaagcgtat tcataaaggc ctggtggtgc gtaaaggctg gctgggtaaa
    43021 ctgccttcat taccgcttcg ctggcgggcg cgtggagtga tgaccctgat gtttatcttg
    43081 ctggcggcca tgctttggtt tgttgctgcc ccggtggtga cgtatttcct ctgtgtgtta
    43141 gtggtattgt tggcaagcac ttgttttgaa tggcagattg tgcgcccgat agaaaatgtc
    43201 gcccgtcagg cactgaaggt ggcgaccgga gagcgtaata gtgttgagca cctgaatcgc
    43261 agcgatgagc tggggctgac attacgcgcg gtagggcagc ttggcctgat gtgccgttgg
    43321 ctaattaacg atgtctcaag ccaggtgtcc agtgtcagaa atggcagtga gacgctggcg
    43381 aaaggcaccg atgaactgaa cgaacatacc cagcagacag ttgataacgt tcagcaaaca
    43441 gtggcgacca tgaaccaaat ggcggcgtcg gtgaaacaga actctgccac ggcgtcggct
    43501 gccgataaac tttctatcac cgccagtaat gcggcagtgc agggtgggga ggcgatgacc
    43561 acggtgatca agacaatgga cgatatcgcc gacagtaccc agcgcattgg caccattact
    43621 tcgctgatta acgatattgc gtttcagacc aatattctgg ccctgaatgc ggcggtggaa
    43681 gcggcgcgtg ccggcgaaca gggcaaaggt tttgcagtgg tggcagggga agtgcgtcat
    43741 ttagccagcc gcagcgctaa tgctgccaac gatattcgca agctgattga tgccagtgct
    43801 gataaggtgc aatccggttc gcagcaggta cacgccgccg gacggacgat ggaagatatt
    43861 gtggcacagg tgaaaaacgt cacccagttg atcgcccaga ttagccattc aacgctggaa
    43921 caggccgatg ggctttccag cctgacccgt gcagtggatg agcttaacct gatcacccag
    43981 aaaaatgccg agctggtgga agagagtgcg caggtgtcgg cgatggtgaa acaccgcgcc
    44041 agccgactgg aagacgcggt gacggtgctg cattaatcgg tatgccggat ccggcggttg
    44101 gagcacaatg cctgatgcga tgctggcgca tcttatcagg cctgcggggt gtgtagcggg
    44161 tgtaggccgg ataaggcgcg tcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg
    44221 atgcggcgat taatccagtt gcgtaatatc aaaagcggcg cggtcgatca ccgcaatgat
    44281 cttttttatt tgtgcatcac tgatatcgct ctggttgacg cgtaaatcca gcaccgcttt
    44341 aaaattatcc agcgcccgct tcatttgcgg gttctggcgc agcgccgcgc caacgcaacg
    44401 cgctttgatg cgttcttcaa tcatctccac ctgttcgcgg ttttcttcca gccactgcgc
    44461 gccttgttcg gtcagcgcaa tctgcttctt accaccttcc tcttcgcgga tggtaatcag
    44521 cgactgctcc tgcagaaaat ccagcgttgg gtagatgacg cccgggcttg gggtgtaatt
    44581 cccctgggtt aggttctcaa tcgctttaat caattcgtaa ccgtggctgt catcgcgcga
    44641 gagaatatcc agaatcacca gacgcaattc accgtgacca aagaaacgtt gccgacgacc
    44701 gccgccgcga ccgtggcgac caccgcagca ttgaccgtgt tcatgctggt gaccgtgtcc
    44761 gcagtgctcg tgttctgact tctcaccttt gcagcagtcc tcatggcgtg gctggccttc
    44821 atgtttacaa cacccttcgt gatgatggct catatcagcc tccttttgct tgattagata
    44881 tatctaattt atatctgatg tgagcattac tgcaagtaca aaaatgattt ttaaacgtgg
    44941 aagcacctca catccaggcc ggataagacg ttcacgccgc atccggcagc attgctatgg
    45001 cactgaatgc cttcacatac ttcagatata tcagttctac aaatcgcttg catttatcat
    45061 gattaacaat cattatcatt tacgaaaatt atttagatat atctgattac tatcacgaag
    45121 gcgataacaa tgaataactc cccccgctac ccgcagcgcg ttcgcaatga tctgcgcttc
    45181 cgtgaactga ctgtgttacg cgttgagcgc atcagcgccg gttttcagcg cattgtcctt
    45241 ggcggcgagg cgctggacgg ttttacatcg cgcggctttg acgatcacag caaactcttc
    45301 tttcctcaac ctgacgctca ctttgtgccg ccaacggtaa cggaagaggg catcgtctgg
    45361 ccggaaggac cacgcccacc gtcgcgtgac tatacgccgc tgtatgacga actacgccat
    45421 gaactggcga ttgatttctt tattcacgac ggtggggtcg ccagcggctg ggcgatgcag
    45481 gcgcaaccgg gcgataaact tacggtggca ggtccgcgcg gttcgctggt ggtgccggaa
    45541 gattacgcgt atcagctgta tgtctgcgat gaatccggaa tgcccgcatt gcgccgccgc
    45601 ctggaaacgt tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt tagcgtgcgg
    45661 gataacgcct gtcaggatta tctcgcgcac cttgatggtt ttaatatcga atggctggca
    45721 catgatgagc aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc cgatgattac
    45781 ttcatctgga taaccggcga aggtaaagtc gttaagaatt taagccgccg ctttgaagcg
    45841 gaacagtatg acccacagcg ggtacgtgca gcggcttact ggcacgcaaa gtaactgata
    45901 tataaaaaga aaaaggctga cgatttctcg tcagcctttg ctatatctgg tggcccctgc
    45961 tggacttgaa ccagcgacca agcgattatg agtcgcctgc tctaaccact gagctaaggg
    46021 gccatggtag cggattataa agtaactccg tgtcgcaatc cagccattac cgtgcgcctg
    46081 ctgtttttat aaacaatgta ttttcaatcc gttatacttt tcctggtgat gttagaaagg
    46141 agtaaatatg gttgaggata ttttggctcc agggttacgg gtcgtgtttt gcggtatcaa
    46201 ccctgggctt tcatccgccg ggactggttt tccctttgct catccagcaa atcgcttctg
    46261 gaaggtgata tatcaggccg ggtttaccga ccgtcagttg aagccgcagg aggcacagca
    46321 tctgctggat tatcgttgtg gcgtcaccaa actggtagac cgtccaacgg tgcaagccaa
    46381 tgaagtttca aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa aaattgaaga
    46441 ttatcagccg caggcgttgg cgattctggg caaacgagca tatgaacagg gattcagcca
    46501 gcgcggtgca cagtggggga aacaaacgct caccattggt tcgacgcaga tttgggtgct
    46561 gccaaatccc agcggtttaa gtcgcgtttc actagagaaa ctggttgaag cgtatcgcga
    46621 gctggaccag gcgctggtag tgcgtgggcg ataaaaaacg ccaccgaaaa ggtggcgttt
    46681 gtgcaaaggg actagtgccg ggtgcggcgt aacgcctgat ccggcctacc gattaatcgt
    46741 ccaggaagct acgcaacact tcagaacggc tcgggtgacg cagtttgcgc agcgccttcg
    46801 cttcgatctg acggatacgt tcgcgggtaa cgtcgaactg tttacccact tcttccagcg
    46861 tgtggtcggt gttcatatcg ataccgaaac gcatacgcag aactttcgct tcacgcgcgg
    46921 tcaggccagc cagcacgtcg tgcgttgccg cacgcaggct ttcggtggtc gcagaatcca
    46981 gcggcagctc gagggtggta tcctcgatga aatcccccag atgcgaatct tcatcatcac
    47041 cgatcggcgt ttccatggag attggctctt tggcgatctt cagcactttg cggatcttgt
    47101 cttccggcat cagcatacgt tcagccagtt cttccggcgt cggttcacgg cccatctctt
    47161 gcagcatctg gcgagaaata cggttgagtt tgttgatagt ctcaatcata tgcaccggaa
    47221 tacggatggt gcgcgcctga tccgcgatag aacgggtgat cgcctgacgg atccaccagg
    47281 ttgcgtaggt ggagaacttg taaccacggc ggtattcgaa tttatcaacc gctttcatca
    47341 gaccgatgtt gccttcctga atcaggtcaa ggaactgcaa gccacggttg gtgtatttct
    47401 tggcgataga aataaccaga cgtaagttcg cttcaaccat ctctttcttc gcacggcggg
    47461 ctttcgcttc accgatggac atacgacggt tgatatcttt aacctgctcg atggtcaggc
    47521 cggtttcttc ttcaatctgc tgcagtttct gcagggcgcg atgcacttct tcagagacat
    47581 cgtgcagttt ttccgaccac ggcttgttca tcgcaattgc cgcgttgaac caggtatcgc
    47641 tggtttcgtt gccggtaaac agggtaatga agtttttctt cggcattttg cactgctcaa
    47701 cgcagagctt catgatcaga cgttcttgcg tacgaacgcg gtccatcatg acgcgcatgc
    47761 tgttgaccag gtagtcaaac tgcttcggca ccaggcggaa ctgtttgaat acttcagaca
    47821 gtttcaggat ctcttcctga gcggcagcgt gactgcgacc tttcgctttg atggtgtcac
    47881 gcgttacaac gtactgagcg cgtagttccg caaatttttc gcgagccagt tccgggtcga
    47941 tgctgttgtc atcatcggcg ctgtcgtcat cgccatcttc ttcgtcttca tcttcgtcat
    48001 cgtccagatc ttcctgggaa agctcagaac cgacgtgagt ggcggtaggt gccagatctt
    48061 cttctgcgtt cgggtcaaca aagccggtga tcagatcgga cagacgcgct tcttctgctt
    48121 caacacgatc gtactgttcc agcagatagg tgatcgcttc cggatattca gcaacggagc
    48181 attgaacctg gttgatcccg tcttcaatac gcttagcgat gtcaatttcg ccttcgcggg
    48241 tcaacagttc aacggtgccc atttcacgca tgtacatgcg taccgggtca gtcgtgcgcc
    48301 cgatttcaga ttccacgctg gaaagcacct gcgcggcggc ttcggcagca tcttcgtccg
    48361 cggtgttttc agccagcatc agatcatcgg catccggtgc ttcttccatc acctgaatgc
    48421 ccatgtcgtt gatcatttgg atgatgtctt cgatctgatc tgaatcgacg atatcttccg
    48481 gcagatggtc attgacctcg gcataggtca gatagccttg ctccttacca cgggtgacaa
    48541 gaagtttcag ctgtgactgc gggttttgct ccataagacg gtatccacac ttatttcatg
    48601 aggttggtgt tgggcgattg acccgatgtc cttcagccgt taacgctgaa atccaacggt
    48661 tgtttaccgc caacgataat tacgagggcg tacttatata tttgccgctg cctctcagtg
    48721 cggctgtcgg gggcttcccg atcgctcttc ggcacttaag ccgttaaatc actttttcgc
    48781 cagctcctgg tttaatgtcc agagctccag gcgttcttcg ttgcttaaac catgcgtgcg
    48841 ctcacgagcg attaactctt cctggcgcag ttcaagcagc gaatcaaaca tatggttgag
    48901 tgagtcggtg aaggtttgct cagcaatatt cttatctgct atatcgtccc acatcgacag
    48961 tttttcaagg gtggcagcat tatttgtacc acgatagtgc tctaaaagtt gcccggtggt
    49021 cagacctggc tgggagagac aagtgttgac cagttctctg aataagccaa gtccagggag
    49081 cttattttca tccagattct caagcggcgg gaccaacgtc gctaattctg gattttgcac
    49141 cagcaaccct ataagtatac gcatggtcgt gcgttttagc tgcggaacag ggcgagaaac
    49201 gccgctctct gccgcttttg gcattaatcg ttcaagctgg ctgtcatcaa gtatgcctaa
    49261 tttgttgcct aattcctgac gaagatatat tcgcagcgtt tcgcccggca cttgcgatat
    49321 caatggcagt gccagcgtac tcaaacgtgc gcgcccgtca ggggtactca gatcaacttg
    49381 cggcatcagg ctgttaaaca gaaatgcgga gagtggcatc gcctgctcca tccgcgcttc
    49441 aaacgcttct ttaccttctt ttcgtaccag cgtgtcaggg tcttcgccat ccggcaaaaa
    49501 cataaagcgt agctgacggc cgtctgtcat gtaaggcagc gccgtttcca gcgctcgcca
    49561 tgcggcatcg cggcctgcac ggtcgccgtc ataacagcaa atgacattgt tggtcgcgcg
    49621 gaacaacagt tgtatgtgat cggcggtggt tgacgtacct aacgacgcaa cggcgtaatt
    49681 aatgccgtat tgcgccagcg ccaccacgtc catatagcct tcgaccacaa gcagacgatt
    49741 gggttcagcg ttatcctgct gcgcttcata aagaccgtaa agctggcggc ctttatggaa
    49801 aatgtctgtt tctggcgagt tcaggtattt gggggtatcg ttgcccagca cgcgcccgcc
    49861 aaaaccaatc acccgaccgc gtttatcgcg aatggggaac atcacccgct cgcggaaacg
    49921 atcgtaactg cgtccctgat cgttagtgac caacatcccc gcatcaatca atgactggcg
    49981 attttctgga ttgccgccaa accgcttcag gacgttgtcc cagccggggg gcgcaaaacc
    50041 aatcgcaaag cgagcgataa cctcgtggct taatccgcgt ttttccagat actggcgcgc
    50101 agacgtggca acaggttgtt gtaaagattg ttggtaaaac gtattcagac cgtccatcaa
    50161 ctgataaagc gtttgcctct gatggcgctc gatctggctg gggccgctgc ctgcttcaaa
    50221 tggcacttca agattgtgca ttgctgccag ctcttcgacc gtttcaacga actcgagctt
    50281 gtcgtagttc atcaggaagt cgatcgcgtt gccgtgcgcg ccacatccaa agcagtggta
    50341 aaactgtttc tcaccgttaa cggtgaatga cggggttttc tcgttgtgga atggacaaca
    50401 cgcgtggaaa ttcttgccct gctttttcag cttcacacgg gcatcgatca gatcgacgat
    50461 gtcagtgcgt gccagcagat cattaatgaa tacgcgtggg attcgtccag ccataggccc
    50521 cgatttttag caattcataa acgaaaataa gccgcgcatt cctttcggaa gcacggcctt
    50581 acaactacaa ctcggtctga tcggagagca acgctctcgg ggaattagta cagacgagtg
    50641 cggcgtgcgt tttcgcgagc cagtttcttc gcgtgacgtt tcactgcaga agctttagcg
    50701 cgcttacgtt cggtagtcgg tttttcatag aactcacgac gacgaacttc cgccagaaca
    50761 cctgcttttt cgcaggaacg cttgaagcga cgcagagcta cgtcgaacgg ctcgttttca
    50821 cgtactttaa ttaccggcat gtgcctctca cctttgatta attcggtttg ccgctggcat
    50881 caacgccagc ttatttcaaa atggtgcgga attttactgc aattgctgct gctttgtaaa
    50941 gcaccgcggc ctttttgaaa gggactttta taagggtgag gagtatacac gaggctttct
    51001 ccaggggcga acaaagtttt acatcaaccc gcattggtcc tacactgcgc ggtaataaag
    51061 cgaggtaaaa caagtcatgc gtgtactggg tattgaaact tcctgcgatg aaaccggcat
    51121 cgccatttac gacgatgaaa aaggtttgtt agccaaccaa ttgtatagtc aggtgaaatt
    51181 gcacgctgac tacggcggcg tcgtgcctga actggcctcc cgcgatcacg tgcgcaaaac
    51241 cgtaccgttg atccaggcgg cgctaaagga gtctggctta acggcaaaag acattgatgc
    51301 tgtggcctat accgcaggcc ctggattagt cggcgcactg ctggttggcg caaccgtggg
    51361 gcgttctctg gcgtttgcct ggaacgttcc ggcaatcccg gtacaccata tggaagggca
    51421 tctgttagcg ccgatgctgg aagataaccc gccggaattt ccgtttgtcg cgctgctggt
    51481 gtccggcggt catacgcagt taatcagcgt gactggcatt ggtcagtacg agctgctcgg
    51541 cgagtctatc gatgatgccg ccggtgaagc gtttgataaa accgcgaagc tgctggggct
    51601 ggattatcct ggcggaccgt tactgtcgaa aatggcggct cagggtactg ccgggcgctt
    51661 tgttttcccg cgtccgatga ccgaccgtcc ggggctggat ttcagcttct ccggtctgaa
    51721 aaccttcgcg gcaaatacca ttcgtgacaa cggcaccgac gaccagacgc gtgctgatat
    51781 cgcccgcgcc tttgaagatg cggtggtcga tacgctgatg attaagtgca agcgagcgtt
    51841 ggatcagact ggctttaagc gactggtcat ggcaggcggc gtgagtgcta accgtacgtt
    51901 acgggcgaag ctggctgaaa tgatgaaaaa acgccgcggc gaagtgttct acgcgcgtcc
    51961 ggagttttgt actgataacg gcgcgatgat cgcctatgcc ggaatggtgc ggtttaaagc
    52021 aggcgcgacg gcggatctcg gcgttagcgt gcgtccgcgc tggccgctgg cggagttacc
    52081 ggccgcgtaa aactatcggg tgccggagag caatttccgg caccgtcctt acttaaagca
    52141 acaccacggg catccacagt aagccggtaa tcaccagcaa tacgaggaat atcagcccaa
    52201 aaatcgcccc cagtcgccag taatccgccg ttggcagata accactaccg tagtaaatcg
    52261 gactgggtcc ggtggcgtat ggtgtaagaa tgctccccag accaattgcc gcgccaacca
    52321 tcaggcagaa caccggcagc gggatttccg gcatcgccag cgcagcggca atcatcatcg
    52381 gtgcgagggc ggaggtatac gccgtggcgc tggcgaaaaa gtagcgcagc agataaaaca
    52441 ccacaatcaa cgccaccatc accatcgttg gcgaataacc gcttaagctg cctgctaaca
    52501 gtttgccaaa ccagctaata aaaccggtgt tgttgagtcc ggtagcgagg gtgataagcg
    52561 aggccagcca gaagaaaacg ttccacgccg ctttattact gacaatgtcg tcccaactga
    52621 taatgcgcag aagcagcatc agtgccacca cgctgtaacc gaccatcgcg gcatcgatat
    52681 aatcaccgcc gaaaatccac agcaccagcg cgcctaccat cagccccagc atccgttttt
    52741 cacgcgaaca gagcgggccc attgcctgca gttccgtctc tgcccagcgc ggcacctgat
    52801 cgccagactt cagtaccggc gggtacagca cataagccag ccagggaacc agcagcacca
    52861 gcaaaatact gagcggcaac atcccgagga accagtcgcc ccaactcagc gtggcgtgag
    52921 atgcgctttt catcagtcca attaacagca agtttggtgc catcgccgtc aagaaaatgg
    52981 cgctggtcac gcagtcggca acaatcccca tccacatgat gtacgagcca atgctgcgcg
    53041 aactgctgtc gtttggttgt gattgataga gcggtggcag gttacggatg atgggataga
    53101 taatccccgc accacgcgcc gagttggacg gtgttacagg tgcgaggatc agctcggaga
    53161 acatcaccgc atagccgaga aacagcgtgc gatgtcccat ctttttcacc agaatcagcg
    53221 caatgcggcg tccaagcccg gttttttcat agcctgtgcc aaacataaag gcggcgaaaa
    53281 tcagccagat aaccgaatta gaaaaaccgg aaaccgccca cgagagggat tttgcagtga
    53341 atttaaagcc tggctgggcg agctgttcgg ggctgaacag cagccaggga gagagtatgg
    53401 cgatgatgga gatacccacc atcgccacca cggcacccgg cacgggttcg aggatcagtc
    53461 caacgatcac gccagtaaaa acggcaaagt agagccaggt atggttctcc agccctgcgg
    53521 gaaccggaag tagagcaata atggcgatga ccgccagcgg cgcaagatat cgccaccatt
    53581 cagtggaagg tttcatcgtg ttatgcctgc gaaaaaggag aggggtgagc ctctccggga
    53641 gggttatttg atgtaatgga cgtgctcgca gatctcttcc acgatgggat cgcggcgttc
    53701 ggcgaacagc tttttgtttt cggctatcag gttgttgccg tgggtatcaa tagagacaat
    53761 cagcgggccg aactctttga cccggcagac ccacagtgac tccggcatcc cgagttctgt
    53821 ccagtgcact tcttcaatct cttctacctg ggttgccgcc agcaccgcgc agcctgccgg
    53881 gaaaatcaca tgtagcgcct tgaatttctg gcagccttct tcggtcagcg gccccatacc
    53941 acctttgcca accaccagtt tcacgccggt ctgctcaata aattcacgtt caaaactttc
    54001 catacgcata ctggttgtcg ggccgacgga gaccatctcc catttatcgc cgtttttgcg
    54061 cacgatgggg ccagcgtgga aaatcgcttt gccgttgaga tcgtaaggga tcggacgttt
    54121 cagttcgatc aagcggcggt gacaaacgtc acggcaggtc accagcgtac cggtcaggta
    54181 gatcacatcg ccgacgcgaa tatcttgcag atcttcagct ttgatcgggg ttgtcaggat
    54241 ctttttcata acgcgctccg ggtgtgagac agattttcaa aggtgagatc ggcatgaacc
    54301 agcagagtgc cgcgacgatg cgcccagcag ccggtagaga cagcaacgcc gatggttgac
    54361 ggatggcggg cggcagattc gatatgtacg cccatcactg aactgttgcc ggtcagccct
    54421 tgtggaccaa tccccagacg gttgagtcct tcttccaggc gtagctccag ctctgccgct
    54481 tttggattgg gatggcggct gccaatcgga cgcaaaatgg ctttacgcga gagtacggcg
    54541 gcggtttcca ccgaggtggc gatgcccacg cccaccagta ccggtggaca ggcgtttacg
    54601 gcgagggtgg agatattttc gaagacgaat ttcaccacgc cttcgtagcc ttctgacggc
    54661 attaacactt tcgagcggcc aggtagcgtg cagccgccgc ctgccatgta aacttcgatt
    54721 tctgcatcgt cattgtcggg gatgatgtcc caggtgaccc acggtacgcc gctaccggta
    54781 tttttgccgg tgtttacttc gtcaaaaatt tctaccgcat tgtgacgtag tggcgctttg
    54841 acggttgcct cttccacggc ttgtttgagt atgctttgca gctcgccaag cagtgggaag
    54901 cgggaaccga ctttaacgaa gaacataatc tccccggtgt cctgacaggc aggacgattc
    54961 aggtcaatcg ctttttgcat gttgtcgaac atcgtatggt agataatttt ccccatcgac
    55021 gacgtttcgg catcctttag ctgttttagt ttatccacca cgtcatcagg cattcgggta
    55081 gaaatcatgg cggtaaagtt agcgacaatc tctgtcaact tattcactgc ctgttgctta
    55141 ttactttcgc tcatcatggc aactccagtt tttgtatttg tctggaaaaa ttcaccgcca
    55201 caaactacca ctaaaaatgt ggttttatat tttttgccac cgtgaatatg taattaacag
    55261 ttcgtgaatc aacgcgaaaa taagaatgaa tattgcgcca gagcagggaa tggcaatgtt
    55321 ttgcgaaata cactgcgctt atcacaactg cggataatta acgatgctga atagctggcc
    55381 tttagccaaa gacttgcagg tactggtaga aatcgtccac agcgggagtt ttagtgctgc
    55441 agcggcgacg cttgggcaga cgcctgcatt tgtcaccaag cggatccaaa tccttgaaaa
    55501 tacgctggca acaacgctgc tgaaccgctc cgctcgcggc gtggcgctga ctgaaagtgg
    55561 ccaacgttgt tacgaacatg cgctggaaat cctcacccaa tatcagcgac tggtcgatga
    55621 cgtcacgcaa atcaaaacgc gcccggaggg gatgattcgt attggctgta gttttggttt
    55681 tgggcgcagc catattgcgc cagccattac cgaacttatg cgcaattatc ctgagttaca
    55741 ggtgcatttt gaattgttcg atcggcaaat tgatttagtg caggataata ttgatctgga
    55801 tattcgtatt aatgacgaaa ttcctgatta ttatattgcg catctgttaa cgaaaaataa
    55861 aagaatatta tgcgcagcac ctgagtatct gcaaaaatat ccgcagccac aatcattaca
    55921 ggaattaagt cgtcatgact gtctggtgac caaagagcgc gatatgaccc acggaatatg
    55981 ggagttgggg aacggtcagg agaaaaaatc ggtgaaggtt agcgggcatc tttcctccaa
    56041 tagcggcgag atagtgttgc aatgggcgct ggagggcaaa gggattatgc tgcgttccga
    56101 gtgggatgtg ttgccgtttc tggagagtgg caaactggtg caagtattgc cggagtatgc
    56161 acaaagcgcc aatatctggg ctgtttaccg ggagccgctc tatcgcagca tgaaattacg
    56221 tgtctgcgtg gaatttctgg cggcatggtg ccagcaacgg ctgggcaagc ccgatgaagg
    56281 ctatcaggtc atgtagatcc accagaaatc actcgggatc cttttcgcgc tttcttttga
    56341 atttcgtcca gatttttgtc tcctgacgac gccacagacg ctggatgttg tcatgatgac
    56401 gcagcaggat caggcaagag agcatcgaaa ccgggaaggt gaattgtggc ttaaaccacc
    56461 atacataaaa cggagcaatc agtgcactga caatcgctcc cagcgacgag tatccgctca
    56521 atagcacggt cagtaaccag gttcccgcca ttactccggt gagatcccag ccaatgggtg
    56581 cgatggcacc aaaagcggta gcaacgcctt ttcctccttt aaatccgaag aaaacgggcc
    56641 agatgtgtcc aagacaggcg gcgatggcaa ttaagcctag ccagaagggg ctgacaccta
    56701 attcatacgc gccccagacg ggcaacattc ctttcagaac gtcgaaaatc agtactgcta
    56761 cggctgctcc cttgccaccg atacgtaaca cattggttgc gcctggattg ccggagccgc
    56821 tggttcgcgg atcgggcagc ccacacaagc ggcaaaccag aatggcactg gaaatggagc
    56881 cgcagaggta cgcgatgagg atcattccag gcgcgattgc actcataagc tgttccgttt
    56941 tgaaaattcg tgttcaacga tgaatctgtg gataatacgc acatttcgcc ggaagtggta
    57001 tccggttagc caaaaagcag gcaggacgtg atggatattg tatttataga gcaactttcg
    57061 gtaatcacca ctattggtgt ttacgactgg gaacagacca tcgaacagaa gttagtgttc
    57121 gatatcgaaa tggcatggga taaccgtaaa gcggcgaaaa gtgatgatgt ggcggattgc
    57181 ctcagttacg ctgacattgc agaaacggtg gtcagccacg tcgagggggc gcgttttgcg
    57241 ctggtggaac gcgtggctga agaggtggcg gagctgctgt tagcacgctt caactcgccg
    57301 tgggtgcgta tcaaactcag caagccaggc gcagtggcgc gggcggcgaa tgttggcgta
    57361 atcattgagc gtggcaataa tctgaaagaa aataattaat tttacagctg ttaaaccaaa
    57421 cggttataac ctggtcatac gcagtagttc ggacaagcgg tacattttaa taatttaggg
    57481 gtttattgat gagcgatatg cactcgctgc tgatagcggc aatattgggt gtggtcgaag
    57541 gattgacaga atttctgccg gtatccagca cgggccatat gattattgtc ggtcacttgt
    57601 tggggttcga gggcgacacg gcgaaaacct ttgaagttgt gatccagtta ggatcaattc
    57661 tggcggtagt agtgatgttc tggcggcgtc tgtttggcct gattggcatc cactttggcc
    57721 gcccgttgca gcacgaagga gaaagcaaag gtcgtttaac gctgatccac attttgctgg
    57781 ggatgattcc ggcggtggta ctggggctgt tgttccacga cacgattaag tcattgttta
    57841 acccgataaa tgtgatgtat gcactggtcg ttggcggttt gttgctgatt gccgccgaat
    57901 gcctgaagcc gaaagagccg cgtgcgccgg gtcttgatga tatgacctat cgtcaggcgt
    57961 ttatgattgg ctgtttccag tgtctggcgc tgtggcctgg tttctcccgt tccggggcca
    58021 ccatttcagg tgggatgctg atgggcgtga gccgttacgc tgcttccgag ttctcgttcc
    58081 tgctggcggt accgatgatg atgggcgcaa cggcgctcga tctctacaaa agctggggct
    58141 tcctgacaac cggcgatatc ccgatgtttg ccgttgggtt tatcaccgct tttgtggtgg
    58201 cgctgatagc gattaaaacc ttcctgcaat tgattaagcg catttcgttt atcccgttcg
    58261 ccatttatcg ctttattgtg gcggctgcgg tgtacgtcgt gttcttttga ttgagaaaag
    58321 gttcgggagc aggcctggtt ttctccctcg ctctgtaggg agagggccgg ggtgggggtg
    58381 attgttcgca ccggtgctgg tctgttcccc tcaccctaac cctctccccg aaggggcgag
    58441 gggatggttc gtgttaacgt ttagttttat actcagtcag gctttgggca acgttgttcc
    58501 ttccaactgg ctaccgccgc aatccgccgt cgggtcagct cctcgcgaat ctccacacct
    58561 ttaaatcccg cttcaacgac cgcttttgtc ggcactgact gcgccacttc ccaggcttcg
    58621 cgcaaccaac ggccttgcgg gtagtccgct gattcaaaac cggttctgcc gcgcacgtca
    58681 gcctcgctgg tcagcgccag ttgctcgaca cgctgcggtt tacgccaggc atcgatggaa
    58741 tcaaacaatt tgacgatagt ttttgggttc agcattggga aggtgtggat gagatcgtga
    58801 aactcagcca ccagtctggc taaatcgcga atttcatttg gcacgcgcag acgctggcag
    58861 agttgttcca ctaatttcac acccgccgga ccgtgaccat gatgacgcgg ccaaagttct
    58921 ggcggcgtca gtcctttacc gagatcgtgg cataaagttg cgaaacggac atcgacctgc
    58981 ggactcagca tcgccgccat tgagagcgtc attaaggtat gaatacccgt gtcgatttcc
    59041 ggatgccacc tggcaggggc cggaacgcca aacagtgcgt caatttccgg gaataaaacg
    59101 cgcagtgcgc cacaatcgcg cagtacctgg aagaacacct gtggattgcg ggtggtgagg
    59161 gcgctttccg tctctttcca tacccgttca ggcgtcaggt gttccagttc acccgcatgg
    59221 gtcatctcgc gcatcagcgt cagagtttca tcggcaatac gaaaaccgag gtgggcataa
    59281 cgcgcagcaa aacgcgccac gcgcagaacg cgtaacggat cttcgccaaa agcgggggaa
    59341 acatggcgca acagacgatt ttgcagatcg cccagaccgt tgtacgggtc gataatctcg
    59401 ccgttatcgt cctgggccag cgcattaatg gtcagatcgc gacgcttaag atcatcttcc
    59461 agcgtgacat ccggtgcggc atagcaagta aaaccggtgt aaccggaacc ggatttccgt
    59521 tcggtacgtg ccagcgcata ctcttcatgc gtttgcggat gcagaaacac aggaaaatcg
    59581 cggcctacct gctggtagcc cgcgtcgagc atctcctgtg gcgtactgcc gaccaccacc
    59641 caatctctgt ctttgaccgg tagccctaac aatgcatccc gaacagcacc accgaccaga
    59701 taaatcttca cgccatcatc ccttcgtcaa atacacaatt cctaataata agacagtgca
    59761 acgaagaagg cgatttagtt catccagcga tctttgcgtt tgcgcgttgg gatcaggtgt
    59821 ggcagtacca gaccgagcag caagccaagc cccagcacgc caccgccata cataaaccat
    59881 tgcatgatga tggtgcgctg tttgtcatcc aactgtacgc tggcggcatc gacctttttc
    59941 tgcgcgacaa tcagctcgtt tttcaatttc tgattttctt cttttaatcc gttgatcacg
    60001 ctgtcgctct gcgccacttt ttgctgcatt tctgcggtgc gctgattcca ggtgttatcg
    60061 atattggtga gtttatcggt cagggttttg acctgatttt ccagatctgg cacacgggag
    60121 cgcaggcttg gctcagtgct aagttgtttc aacgggatcc aggcggtacg gccagagctg
    60181 tctttcacct gggcataatt ggtgttggcg tcagtttgta ataaggtcac ttcctcgccg
    60241 gcgttaaccg tgcccacgag gcgataatga tctcccggac cgctacggac ccaggtattc
    60301 agttcatcag aaacatagcg cgtttcttca gcgtgtgaga cggcagtggc gctaagtgcg
    60361 agtaaagtta atccgatcag gcgtaatttt ggcatcaggc tgtcgttatt gtcatggaaa
    60421 gtggaacgat agtagtggca tcagtgtcgc tacgcaaagc attcgacatc aatcggaatc
    60481 ctctgtgtca ggtcttgcca ctgcttcaaa cttttacgcc cgtcaaattg cccgttgcat
    60541 ggcaatttgg cgcaaaatac tatctactga caaaaaagat cgcattaagt tcgccgttca
    60601 tcgtcactta tgtgacataa ccgataagta aaggccatgg ctcaggaaat cgaattaaag
    60661 tttattgtta atcacagtgc cgttgaggcg ttgcgtgacc atctcaatac gctgggcggc
    60721 gagcaccatg accccgtgca gttgctgaat atttactacg aaacgccgga taactggctg
    60781 cgtgggcacg atatgggctt acgtattcgt ggcgaaaacg gtcgctatga gatgaccatg
    60841 aaagttgcag gaagagtgac aggcggctta caccagcgcc cggaatataa cgtggcgttg
    60901 agcgaaccga cgctcgacct ggcgcagtta ccgacggaag tctggccgaa cggcgaattg
    60961 cccgccgatc tcgcttcccg cgtacaaccg ctgttcagca ccgattttta tcgcgaaaaa
    61021 tggctggtgg cggtcgatga cagccgaatt gaaatcgctc tcgatcaggg ggaggtgaaa
    61081 gcgggggaat ttgctgaacc tatctgtgag ctggaactgg aactgcttag cggcgacacg
    61141 cgcgcggtgc tgaaactggc gaaccaactg gtatcgcaaa ccggattacg ccagggcagc
    61201 ctgagcaaag cggcgcgtgg ctatcatctg gcgcagggca atccggcgcg tgaaatcaaa
    61261 ccgaccacca ttttgcatgt tgcggcaaaa gccgatgtgg aacaggggct ggaagcggcg
    61321 ttcgagctgg cgttagcgca atggcagtat catgaagaac tgtgggtacg cggcaacgat
    61381 gcggcgaaag agcaggtgct ggcagccatt ggccttgttc gccatgcact gatgctgttc
    61441 ggtggcattg tgccgcgtaa agcgagcact cacttacgtg atttgctgac tcaatgcgaa
    61501 gcgaccatcg cttctgcggt ttctgccgtg acggcggtct actctaccga aacggcaatg
    61561 gcaaagctgg cgttgaccga atggttggta agcaaagcct ggcagccgtt tttagatgcc
    61621 aaagcgcagg gtaaaatcag cgattccttc aaacgctttg ccgatatcca tctttcccgc
    61681 catgccgctg aactgaaaag cgttttctgc cagccgttag gcgatcgcta ccatgatcag
    61741 ttgccacgcc tgacgcgtga tattgactca atactgctgc tggcaggtta ctatgatcct
    61801 gtcgtcgcgc aagcctggct ggagaactgg caggggctgc gtcacgctat tgcgaccggg
    61861 caacgcattg aaattgaaca tttccgtaat gaggcaaaca atcaggaacc gttctggttg
    61921 cacagcggaa aacgttaatc aggcaaggat aaaatcgctt atgaagccgc tctcttcacc
    61981 gttacagcag tactggcaga ccgttgttga gcggctgcca gagcctttag ccgaggaatc
    62041 acttagcgca caggcgaagt cagtacttac ttttagtgat tttgtgcagg acagcgtgat
    62101 tgtgcatcca gagtggctga cggaactgga aagccaaccg ccgcaggccg acgaatggca
    62161 gcattacgcg gcatggttgc aggaggcgct cagtaatgtg agtgacgaag ccgggttaat
    62221 gcgcgagctg cggctattcc ggcggcgcat tatggtgcgc atcgcctggg cgcaaacgct
    62281 ggctttggtt actgaagaga gcatattgca gcagctcagc catctggcgg agacgctgat
    62341 tgtcgccgcg cgtgactggc tgtatgacgc ttgctgccgc gagtggggaa cgccgtgcaa
    62401 tgcgcagggc gaagcgcaac cgctgctgat tttaggcatg ggtaagctgg gcggtgggga
    62461 actgaatttc tcctcagata tcgatctgat ttttgcctgg ccggaacatg gttgtacgca
    62521 gggcggacgc cgggaactgg ataacgcgca gttttttacc cgcatggggc agcggctgat
    62581 taaagtgttg gatcaaccaa cgcaggatgg cttcgtctat cgcgtggata tgcggctgcg
    62641 tccgtttggc gaaagtggcc cgctggtgct gagctttgcc gcgttggagg attattacca
    62701 ggagcagggg cgcgactggg agcgttacgc gatggtcaag gcgcggatta tgggcgatag
    62761 cgaaggcgtc tatgctaacg agttgcgtgc gatgctgcgc ccgtttgttt tccgtcgtta
    62821 catcgatttc agcgtgattc agtcgctgcg caacatgaaa gggatgattg cccgtgaagt
    62881 gcgtcgacgt ggtttgaccg acaatatcaa actcggcgca ggcggcattc gcgaaattga
    62941 atttatcgtt caggtgttcc agctcattcg cggtgggcgc gaaccgtcgc tgcagtcgcg
    63001 ctctttactg ccaacgctca gcgtcattgc cgcgctgcat ctgctttctg aaaacgatgc
    63061 tgaacaattg cgagtggcgt atctgttcct gcggcgtctg gaaaacctgc tgcaaagcat
    63121 taacgacgaa caaacccaga cgcttccttc tgatgagctt aaccgtgcgc ggctggcgtg
    63181 ggcgatggac tttgctgact ggccgcaact gaccggggcg ctgaccgcac atatgaccaa
    63241 tgtgcgccgg gtgtttaatg aattgattgg cgacgacgaa agtgaaactc aggaagagtc
    63301 gctgtcggaa cagtggcgtg agctgtggca ggatgcgttg caggaagatg acactacgcc
    63361 agtgctggcg catcttagcg aggatgatcg caaacaggtg ctaacgctga ttgccgattt
    63421 ccgcaaagag ctggataagc gcaccatcgg gccgcgagga cgtcaggtgc tcgaccatct
    63481 gatgccgcat ctgctaagtg atgtctgtgc gcgtgaagac gctgccgtta cgctgtcgcg
    63541 cattaccgcc ttgctggtgg ggattgttac ccgcaccacc tatttagaat tgctaagtga
    63601 attccccgcg gcgcttaaac atttgatttc tctgtgtgcc gcgtcgccga tgattgccag
    63661 ccaactggcg cgttatccat tattgctgga tgaattgctc gatccaaaca ccctttacca
    63721 gccgacggcg accgatgcct accgcgatga gttgcgccag tatttgctgc gcgtgccgga
    63781 agatgacgaa gagcaacagc ttgaggcgct gcgtcagttc aaacaggcgc agctgttacg
    63841 catcgccgca gcggatatcg ctggtacgct accggtgatg aaagtgagcg atcacttaac
    63901 ctggctggca gaggctatga tagatgccgt cgttcagcag gcgtgggttc aaatggtggc
    63961 ccgctacggt aagccaaatc acctcaacga tcgcgaaggc cgtggttttg cggtggtcgg
    64021 ctacggcaag ctgggcggct gggagttagg ctacagttcc gatcttgacc tgatcttcct
    64081 tcatgattgc ccaatggatg cgatgactga cggcgagcgg gaaatcgacg ggcgacagtt
    64141 ttatctgcgt ctggcgcaac gcattatgca tctgttcagt acgcgtacct cttccggcat
    64201 tttgtatgaa gtggatgcac ggctacgccc gtccggggcc gcgggaatgc tggtgacatc
    64261 cgcagaagca tttgccgatt atcagaaaaa cgaggcctgg acgtgggaac atcaggcgct
    64321 ggtgcgtgcg cgggtagtgt acggcgatcc gcagctcacc gcgcactttg acgcggtgcg
    64381 tcgcgagatt atgacgctgc cgcgtgaagg taaaactctg caaacggaag tgcgggaaat
    64441 gcgcgagaaa atgcgcgctc atctcggcaa taaacatcgc gatcgctttg atatcaaagc
    64501 cgatgaaggc ggaattaccg atatcgaatt tattacccaa tatctggtgt tgcgctacgc
    64561 tcatgaaaaa ccgaagttaa cgcgctggtc agacaacgtg cgcattctgg aactactggc
    64621 gcaaaacgac attatggaag agcaggaagc gatggcgctg acccgtgctt atactacgct
    64681 gcgcgatgaa cttcatcatc tggcattaca ggaattgccg ggccatgtgc cggaggattg
    64741 cttcaccgca gagcgtgaac tggtgcgggc aagctggcag aagtggctgg tggaagaatg
    64801 aagtatggta ttatcgcgcg caaattttga atctctcagg agacaggaat gaaagtaacg
    64861 ctgccagagt ttgaacgtgc aggagtgatg gtggttggtg atgtgatgct ggatcgttac
    64921 tggtacggtc ccaccagccg tatctcgccg gaagcgccgg tgcccgtggt taaagtcaat
    64981 accatcgaag aacgtccggg cggcgcggct aacgtggcaa tgaacatcgc ttctctcggt
    65041 gccaatgctc gcctggtcgg gctgacgggc attgacgatg cggcgcgtgc gctgagtaaa
    65101 tctctggccg acgtcaacgt caaatgcgac ttcgtttctg taccgacgca tccgactatc
    65161 accaaattac gggtactttc ccgcaaccaa cagctgatcc gcctggattt tgaagaaggt
    65221 ttcgaaggtg ttgatccgca gccgctgcac gagcggatta atcaggcgct gagttcgatt
    65281 ggcgcgctgg tgctttctga ctacgccaaa ggtgcgctgg caagcgtaca gcagatgatc
    65341 caactggcgc gtaaagcggg cgttccggtg ctgattgatc caaaaggtac cgattttgag
    65401 cgctaccgcg gcgctacgct gttaacgccg aatctctcgg aatttgaagc tgttgtcggt
    65461 aaatgtaaga ccgaagaaga gattgttgag cgcggcatga aactgattgc cgattacgaa
    65521 ctctcggctc tgttagtgac ccgttc
//
LOCUS       DAEVZA010000009        62530 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEVZA010000009 DAEVZA010000000
VERSION     DAEVZA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.009"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(9..683)
                     /gene="yiiM"
                     /locus_tag="LAF92_000627"
     CDS             complement(9..683)
                     /gene="yiiM"
                     /locus_tag="LAF92_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBI9789684.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(832..1815)
                     /gene="kdgT"
                     /locus_tag="LAF92_000628"
     CDS             complement(832..1815)
                     /gene="kdgT"
                     /locus_tag="LAF92_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBI9789685.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(2075..2695)
                     /gene="sodA"
                     /locus_tag="LAF92_000629"
     CDS             complement(2075..2695)
                     /gene="sodA"
                     /locus_tag="LAF92_000629"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBI9789686.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2980..4014
                     /gene="rhaT"
                     /locus_tag="LAF92_000630"
     CDS             2980..4014
                     /gene="rhaT"
                     /locus_tag="LAF92_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBI9789687.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(4011..4877)
                     /gene="rhaR"
                     /locus_tag="LAF92_000631"
     CDS             complement(4011..4877)
                     /gene="rhaR"
                     /locus_tag="LAF92_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBI9789688.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4933..5769)
                     /gene="rhaS"
                     /locus_tag="LAF92_000632"
     CDS             complement(4933..5769)
                     /gene="rhaS"
                     /locus_tag="LAF92_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBI9789689.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            6057..7526
                     /gene="rhaB"
                     /locus_tag="LAF92_000633"
     CDS             6057..7526
                     /gene="rhaB"
                     /locus_tag="LAF92_000633"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBI9789690.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYQRECRSLTLREIHRFKNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            7523..8782
                     /gene="rhaA"
                     /locus_tag="LAF92_000634"
     CDS             7523..8782
                     /gene="rhaA"
                     /locus_tag="LAF92_000634"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBI9789691.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            9024..9848
                     /gene="rhaD"
                     /locus_tag="LAF92_000635"
     CDS             9024..9848
                     /gene="rhaD"
                     /locus_tag="LAF92_000635"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBI9789692.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9858..10172
                     /gene="rhaM"
                     /locus_tag="LAF92_000636"
     CDS             9858..10172
                     /gene="rhaM"
                     /locus_tag="LAF92_000636"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBI9789693.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            10473..10919
                     /locus_tag="LAF92_000637"
     CDS             10473..10919
                     /locus_tag="LAF92_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBI9789694.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            10930..12381
                     /locus_tag="LAF92_000638"
     CDS             10930..12381
                     /locus_tag="LAF92_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HBI9789695.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            12371..13441
                     /locus_tag="LAF92_000639"
     CDS             12371..13441
                     /locus_tag="LAF92_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI9789696.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAVLKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            13441..15189
                     /locus_tag="LAF92_000640"
     CDS             13441..15189
                     /locus_tag="LAF92_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HBI9789697.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDIWPQYPGNEIKQIV
                     EQCGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(15239..16279)
                     /locus_tag="LAF92_000641"
     CDS             complement(15239..16279)
                     /locus_tag="LAF92_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HBI9789698.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRVRDKVPYSDWDKEQLQDANSSWMVDDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(16432..17265)
                     /gene="fdhD"
                     /locus_tag="LAF92_000642"
     CDS             complement(16432..17265)
                     /gene="fdhD"
                     /locus_tag="LAF92_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBI9789699.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            17459..20509
                     /gene="fdnG"
                     /locus_tag="LAF92_000643"
     CDS             17459..20509
                     /gene="fdnG"
                     /locus_tag="LAF92_000643"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:18044..18046,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBI9789700.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            20522..21424
                     /gene="fdoH"
                     /locus_tag="LAF92_000644"
     CDS             20522..21424
                     /gene="fdoH"
                     /locus_tag="LAF92_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBI9789701.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            21421..22056
                     /gene="fdoI"
                     /locus_tag="LAF92_000645"
     CDS             21421..22056
                     /gene="fdoI"
                     /locus_tag="LAF92_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBI9789702.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            22053..22982
                     /gene="fdhE"
                     /locus_tag="LAF92_000646"
     CDS             22053..22982
                     /gene="fdhE"
                     /locus_tag="LAF92_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBI9789703.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(23312..23554)
                     /locus_tag="LAF92_000647"
     CDS             complement(23312..23554)
                     /locus_tag="LAF92_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789704.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(23772..23990)
                     /locus_tag="LAF92_000648"
     CDS             complement(23772..23990)
                     /locus_tag="LAF92_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBI9789705.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(24847..25788)
                     /gene="fabY"
                     /locus_tag="LAF92_000649"
                     /gene_synonym="yiiD"
     CDS             complement(24847..25788)
                     /gene="fabY"
                     /locus_tag="LAF92_000649"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBI9789706.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(25833..26270)
                     /gene="dtd"
                     /locus_tag="LAF92_000650"
     CDS             complement(25833..26270)
                     /gene="dtd"
                     /locus_tag="LAF92_000650"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBI9789707.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(26267..27139)
                     /locus_tag="LAF92_000651"
     CDS             complement(26267..27139)
                     /locus_tag="LAF92_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBI9789708.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(27133..27732)
                     /gene="yihX"
                     /locus_tag="LAF92_000652"
     CDS             complement(27133..27732)
                     /gene="yihX"
                     /locus_tag="LAF92_000652"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBI9789709.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(27831..28616)
                     /locus_tag="LAF92_000653"
     CDS             complement(27831..28616)
                     /locus_tag="LAF92_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI9789710.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(28650..29546)
                     /gene="yihV"
                     /locus_tag="LAF92_000654"
     CDS             complement(28650..29546)
                     /gene="yihV"
                     /locus_tag="LAF92_000654"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBI9789711.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            29714..30610
                     /gene="yihU"
                     /locus_tag="LAF92_000655"
     CDS             29714..30610
                     /gene="yihU"
                     /locus_tag="LAF92_000655"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBI9789712.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            30634..31512
                     /gene="yihT"
                     /locus_tag="LAF92_000656"
     CDS             30634..31512
                     /gene="yihT"
                     /locus_tag="LAF92_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBI9789713.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            31529..32770
                     /gene="yihS"
                     /locus_tag="LAF92_000657"
     CDS             31529..32770
                     /gene="yihS"
                     /locus_tag="LAF92_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBI9789714.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            32908..33810
                     /locus_tag="LAF92_000658"
     CDS             32908..33810
                     /locus_tag="LAF92_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBI9789715.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYHLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            34009..36045
                     /gene="yihQ"
                     /locus_tag="LAF92_000659"
     CDS             34009..36045
                     /gene="yihQ"
                     /locus_tag="LAF92_000659"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBI9789716.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            36091..37476
                     /locus_tag="LAF92_000660"
     CDS             36091..37476
                     /locus_tag="LAF92_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9789717.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPASVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            37519..38922
                     /locus_tag="LAF92_000661"
     CDS             37519..38922
                     /locus_tag="LAF92_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9789718.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            38990..39682
                     /gene="ompL"
                     /locus_tag="LAF92_000662"
     CDS             38990..39682
                     /gene="ompL"
                     /locus_tag="LAF92_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBI9789719.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(39773..41038)
                     /locus_tag="LAF92_000663"
     CDS             complement(39773..41038)
                     /locus_tag="LAF92_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9789720.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(41140..42120)
                     /locus_tag="LAF92_000664"
     CDS             complement(41140..42120)
                     /locus_tag="LAF92_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBI9789721.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDAPLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(42128..42838)
                     /locus_tag="LAF92_000665"
     CDS             complement(42128..42838)
                     /locus_tag="LAF92_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBI9789722.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSSVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(43055..44878)
                     /gene="typA"
                     /locus_tag="LAF92_000666"
     CDS             complement(43055..44878)
                     /gene="typA"
                     /locus_tag="LAF92_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBI9789723.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            45251..46660
                     /gene="glnA"
                     /locus_tag="LAF92_000667"
     CDS             45251..46660
                     /gene="glnA"
                     /locus_tag="LAF92_000667"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBI9789724.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     NDRVRMTPHPVEFELYYSV"
     gene            46834..47883
                     /gene="glnL"
                     /locus_tag="LAF92_000668"
     CDS             46834..47883
                     /gene="glnL"
                     /locus_tag="LAF92_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBI9789725.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            47895..49304
                     /gene="glnG"
                     /locus_tag="LAF92_000669"
     CDS             47895..49304
                     /gene="glnG"
                     /locus_tag="LAF92_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBI9789726.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            49416..49526
                     /locus_tag="LAF92_000670"
     CDS             49416..49526
                     /locus_tag="LAF92_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBI9789727.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(49716..51089)
                     /gene="hemN"
                     /locus_tag="LAF92_000671"
     CDS             complement(49716..51089)
                     /gene="hemN"
                     /locus_tag="LAF92_000671"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBI9789728.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(51278..51787)
                     /gene="yihI"
                     /locus_tag="LAF92_000672"
     CDS             complement(51278..51787)
                     /gene="yihI"
                     /locus_tag="LAF92_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBI9789729.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            52369..53001
                     /locus_tag="LAF92_000673"
     CDS             52369..53001
                     /locus_tag="LAF92_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBI9789730.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(53383..56169)
                     /gene="polA"
                     /locus_tag="LAF92_000674"
     CDS             complement(53383..56169)
                     /gene="polA"
                     /locus_tag="LAF92_000674"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBI9789731.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            56533..57465
                     /locus_tag="LAF92_000675"
     CDS             56533..57465
                     /locus_tag="LAF92_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBI9789732.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(57506..58936)
                     /locus_tag="LAF92_000676"
     CDS             complement(57506..58936)
                     /locus_tag="LAF92_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBI9789733.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(59091..59717)
                     /gene="dsbA"
                     /locus_tag="LAF92_000677"
     CDS             complement(59091..59717)
                     /gene="dsbA"
                     /locus_tag="LAF92_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBI9789734.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(59734..60720)
                     /gene="srkA"
                     /locus_tag="LAF92_000678"
     CDS             complement(59734..60720)
                     /gene="srkA"
                     /locus_tag="LAF92_000678"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBI9789735.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(60797..61066)
                     /locus_tag="LAF92_000679"
     CDS             complement(60797..61066)
                     /locus_tag="LAF92_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBI9789736.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            61136..61720
                     /gene="mobA"
                     /locus_tag="LAF92_000680"
     CDS             61136..61720
                     /gene="mobA"
                     /locus_tag="LAF92_000680"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBI9789737.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            61702..62229
                     /gene="mobB"
                     /locus_tag="LAF92_000681"
     CDS             61702..62229
                     /gene="mobB"
                     /locus_tag="LAF92_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBI9789738.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(62333..62448)
                     /gene="rrf"
                     /locus_tag="LAF92_000682"
     rRNA            complement(62333..62448)
                     /gene="rrf"
                     /locus_tag="LAF92_000682"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        15308 a        16053 c        15519 g        15650 t
ORIGIN      
        1 tccgggggtt attttcccca caaccggcgg gaaaaatctt cgatcttgcc gctcagtcgg
       61 cgcttttgca tcgtccgcgt ccagcttttc gataacccgg cggcggagag taaacggtga
      121 tattgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg cacggtcaca
      181 tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc tggcgctatc
      241 acgctataca accaccccac cttacctgtg ttttgcatca actgcgctat atcgctgata
      301 tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg aattaatgcc
      361 tctccccagc ggaaaatatc gcccatataa acattacttt ccgtgagacc gtcggttgag
      421 agattttcgc caaacgcagg cgcaacaaac aactccgcct gttccggaaa ttcccgcgcc
      481 cagtagagat aatgctcacg aggataatga cacagcgctc tgtctggtcc gccgtgaacc
      541 tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa ctcaccatca
      601 acctggattt tggcaattgc gctgggtttg ccttcgggat aagcctgaat cttgcctgtg
      661 tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc cattagacca
      721 tatttatttt atgtcaggcg tgctgttttc gcggtaagta tccaggcagg aactcaatag
      781 caaaccggac gaacgttagt tcatccggct gtggggatta aggaagcagg tttatttcac
      841 cgtacctaaa atttcgattt tcgctgctct ggctttgact ttacgtgacc agatagaggt
      901 aagaattggc accagaatcg aagtcacaat gaccgccgtc gctaccagcg aagttgctgc
      961 cggagccatc ggtttaaacg caggcaccat ttctgcaatc agcacaggtg tcgctaccgc
     1021 ggcccctgcg gaactggaag cggcaattcc ggcagtgcca tcgccaccgc caatcaattt
     1081 atcggcgata atcaacggaa taccggtcac gataattact gccacaccca acaggatccc
     1141 cagcaaacca gtctgggcaa tcacagtcaa atcaatggta ttgcccagcg cgaaggcaaa
     1201 gaatggaatc agcgtttgca ccgctttgct gaaaaattct cgtaattcag ggtcaaggtt
     1261 ccccagggca aagcccacca ggaacggtaa tactgcgccg acgaaaacat gcggttcaaa
     1321 cgaggcaatc cctgcagtgc ccagaataat catcgtcatg agcggcccgg actccaacga
     1381 catcaacaca aatgccccag cttcttcttt tgtgccgtac tgctgcatga tggaagcgta
     1441 aagtccgcca ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa gtccggcaaa
     1501 gaatccaact tcaacaccat gttccggaat aatgcgcgag gcaattgccg caaccaccca
     1561 cgcgacggca atttttgtta ccaccagcgt accggattta cgcaataccg ttcccgtcgc
     1621 gcttaatttt attgacgccc ccatgcaaaa aaaccacacc gccagaatgg gcaccgtacc
     1681 ggtaatcatc ccgttggtga atgatccaaa atatttcccc gcccccggcg agaaggtgtg
     1741 gcacagtgcg ccaaggaata gcgggacgag catcatcccc cccgggattt tctcaatcga
     1801 gcgttttatc tgcatttcca tcacctgtcg gcatattttt aagggctgat gcgtcattcc
     1861 tcaatggagc atcagtgttg atggaaacga agatagacat tcatttaatc aataaaagtg
     1921 atcaaaacca catcaattga aacactgttt tatttttatc ggatcattgt ttttattttg
     1981 aaattgatca caaaaaaacc accgccgttg gcgatggttc attttttaag ctgatatgcg
     2041 gcctatacgc ctcattgcag caggcggcaa atgattattt tttcgccgca aaacgtgccg
     2101 ctgcttcgtc ccagttcacc acgttccaga actctttaat gtagtccgga cggcggttct
     2161 ggaatttcag gtagtaagca tgttcccaca catccaggcc cagaatcggg aagccggaag
     2221 cgccagaaat agcttcaccc atcagcggag aatcctggtt agcagtagaa accaccgcca
     2281 gtttatcgcc tttcagcacc agccatgccc agccggaacc aaagcgggaa gctgccgctt
     2341 tttcaaattc tgctttgaag ttatcaacgg agccgaagtc acgttcgata gccgctttca
     2401 ggtcaccctg cagggtggtg ccttttttca gacctttcca gaacaggctg tggttagcgt
     2461 gaccgccagc gttgttgcgc agtacggttt tcttgtctgc tggcagctgg tccagtttgg
     2521 tgatcagctc ttcaaccggc aggttggcaa attctggcag gctttccagc gccgcgttgg
     2581 cgttgtttac gtaggtctga tggtgtttgg tgtggtggat ttccatggtc tgcttatcga
     2641 agtgcggttc cagggcatcg taagcatacg gcagggatgg cagggtatag ctcatattca
     2701 tctccagtat tgtcgggcgg ccgattgtta atgccgcgta agcagttggt tcattatagt
     2761 taattaaatg atattgaaaa tgattatcaa tgccgtactt ttcgtaaggg tatggttttg
     2821 caggaaaatg cccgagatgt gaagcaaatc acccacttaa tgccgtgatt gccagtaaat
     2881 cgacaacggc ggcaacaggc gaaaggttaa tcgacagcac gatttttaca ctcatctcgt
     2941 cggagatgtg aagcgacgaa aaatgatgag gataagaaga tgagtaacgc gattacgatg
     3001 gggatatttt ggcatttgat cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa
     3061 aaagtaaaaa aatggtcatg ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt
     3121 attctgccgt gggccatcag cgccctgtta ctaccgaatt tctgggcgta ttacagctcg
     3181 tttagtctct ctacgctgct gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat
     3241 atcaactatg gcctgaccat gcgttatctc ggcatgtcga tgggaattgg catcgccatt
     3301 ggcattacgt tgattgtcgg tacgctgatg acgccaatta tcaacggcaa tttcgatgtg
     3361 ctgattaaca ccgaaggcgg acgcatgacg ttgctcggcg ttctggtggc gctgattggc
     3421 gtagggattg taactcgagc cgggcagttg aaagagcgca agatgggcat taaagccgaa
     3481 gagttcaatc tgaaaaaagg gctggtgctg gcggtgatgt gcggcatttt ctctgccggg
     3541 atgtcctttg cgatgaacgc cgcaaaaccg atgcatgaag ccgctgccgc actaggcgtc
     3601 gatccactgt atgtcgctct gccaagctat gtcatcatca tgggcggcgg cgcgatcatc
     3661 aacctcggtt tctgttttat tcgtctggca aaagtgaagg atttgtcgct aaaagccgac
     3721 ttctcgctgg caaaaccgct gatcactcac aatgtgttac tctcagcact gggcgggttg
     3781 atgtggtatc tgcaattctt tttctatgcc tggggccacg cccgcattcc ggcgcaatat
     3841 gactacatca gttggatgct gcatatgagc ttctatgtat tgtgcggcgg tatcgtcggg
     3901 ctggtgctga aagagtggaa caatgcagga cgccgtccgg taacggtgtt gagcctcggt
     3961 tgtgtggtga ttattgtcgc cgctaacatc gtcggcatcg gcatggcgaa ttaatctttc
     4021 tgcgaattga gatgacgcca ctggctgggc gtcatcccgg tttcccgggt aaacaccacc
     4081 gaaaaatagt tactatcttc aaagccacat tcggtcgaaa tatcactgat taacaggcgg
     4141 ctatgctgga gaagatattg cgcatgacac actctgacct gtcgcagata ttgattgatg
     4201 gtcattccag tctgctggcg aaattgctga cgcaaaacgc gctcactgca cgatgcctca
     4261 tcacaaaatt tatccagcgc aaagggactt ttcaggctag ccgccagccg ggtaatcagc
     4321 ttatccagca acgtttcgct ggatgttggc ggcaacgaat cactggtgta acgatggcga
     4381 ttcagcaaca tcaccaactg cccgaacagc aactcagcca tttcgttagc aaacggcaca
     4441 tgctgactac tttcatgctc aagctgaccg ataacctgcc gcgcctgcgc catccccacg
     4501 ctacctaagc gccagtgtgg ttgccctgcg ctggcgctaa atcccggaat cgccccctgc
     4561 cagtcaagat tcagcttcag acgctccggg caataaataa tattctgcaa aaccagatcg
     4621 ttaacggaag cgtaggagtg tttatcgtca gcatgaatgt aaaagagatc gccacgggta
     4681 atgcgataag ggcgatcgtt gagtacatgc aggccattac cgcgccagac aatcaccagc
     4741 tcacaaaaat catgtgtatg ttcagcaaag acatcttgcg gataacggtc agccacagcg
     4801 actgcctgct ggtcgctggc aaaaaaatca tctttgagaa gttttaactg atgcgccacc
     4861 gtggctacct cggccagaga acgaagttga ttattcgcaa tatggcgtac aaatacgttg
     4921 agaagattcg cgttattgca gaaaaccatc ccgtccctga cgaatatcac gcggtgacca
     4981 gttaaactct cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc cacagcgata
     5041 ggcgatgtca gtaacgctgg cctcgctgtg gcgtagcaga tgtcgggctt tcatcagtcg
     5101 caggcggttc aggtatcgct gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag
     5161 cgtacgcagt gaaagagaaa attgatccgc cacggcatcc caattcacct catcggcaaa
     5221 atggtcctcc agccaggcca gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc
     5281 ctgcaaactg cttttacgca gcaagagcag taattgcata aacaagatct cgcgactggc
     5341 ggtcgagggt aaatcatttt ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac
     5401 ctgctgcaat acgctgtggt taacgcgcca gtgagacgga tactgcccat ccagctcttg
     5461 tggcagcaac tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc gatacagcac
     5521 attggtcaga cacagattat cggtatgttc atacagatgc cgatcatgat cgcgtacgaa
     5581 acagaccgtg ccaccggtga tggtataggg ctgcccatta aacacatgaa tacccgtgcc
     5641 atgttcgaca atcacaattt catgaaaatc atgatgatgt tcaggaaaat ccgcctgcgg
     5701 gagccggggt tctatcgcca cggacgcgtt accagacgga aaaaaatcca cactatgtaa
     5761 tacggtcata ctggcctcct gatgtcgtaa acacggcgaa atagtaatca cgaggtcagg
     5821 ttcttacctt aaattttcga cgggaaacca cgtaaaaaac gtcgattttt caagatacag
     5881 catgaatttt caggaaatgc ggtgagcatc acaccaccac aattcagcaa tttgtgaaca
     5941 tcatcacgtt catctttccc tggttgccaa tggcccattt tcctgtcagt agcgagaagg
     6001 tcgcgaattc aggcgctttt tagactggtc gtaatgaaat tcagcaggat cacattatga
     6061 cctttcgcaa ttgtgtcgcc gtcgatctcg gcgcatccag tgggcgcgtg atgctggcgc
     6121 gttaccagcg tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt aaaaatgggc
     6181 tgcatagcca gaacggttat gtcacctgga atgtggatag cctggaaagt gccattcgcc
     6241 ttggattaaa caaggtgtgc gaggaaggga ttcgtatcga tagcattggg attgatacct
     6301 ggggcgtgga ctttgtgctg ctcgaccaac agggtcagcg tgtgggcctg cccgttgctt
     6361 atcgcgatag ccgctccaat ggcctaatgg cgcaggcaca gcaacaactc ggcaaacgcg
     6421 atatttatca acgtagcggc atccagtttc tgcccttcaa tacgctttat caactgcgtg
     6481 cgctgacgga gcaacaacct gaacttattc cacacattgc tcacgctctg ctgatgccgg
     6541 attacttcag ctatcgcctg accggcaaga tgaactggga gtacaccaat gccaccacca
     6601 cacaactggt caatatcaat agcgacgact gggacgagtc gctactggcg tggagcgggg
     6661 ccaacaaagc ctggtttggt cgcccgacgc atccgggtaa tgtcattggg cactggattt
     6721 gcccgcaggg taatgagatt ccggtggtcg ccgttgccag ccatgatacc gccagcgcgg
     6781 ttatcgcctc gccgttaaac ggttcacgcg ccgcttatct ctcttctggc acctggtcat
     6841 tgatgggctt cgaaagccag acgccattta ccaatgacac ggcgctggca gccaacatca
     6901 ccaatgaagg cggggcggaa ggtcgctatc gggtgctgaa aaatattatg ggcttatggc
     6961 tgcttcagcg agtgcttcag gaacggcaaa tcaacgatct cccggcgctt atcgccgcga
     7021 cacaggcact tccggcctgt cgcttcatca tcaatcccaa tgacgatcgc tttattaatc
     7081 ctgaagcgat gtgcagcgaa attcaggctg cgtgtcggga aacggcgcaa ccgatcccgg
     7141 aaagtgatgc tgaactggcg cgctgtattt tcgacagtct ggcgctgctg tatgccgatg
     7201 tgttgcatga gctggcgcag ctgcgcggtg aagatttctc gcaactgcat attgtcggcg
     7261 gcggctgcca gaacacgctg ctcaaccagc tatgcgccga tgcctgcggt attcgggtga
     7321 tcgccgggcc tgttgaagcc tcaacgctcg gcaatatcgg catccagtta atgacgctgg
     7381 atgaactcaa caatgtggat gatttccgtc aggtcgtcag caccaccgcg aatctgacca
     7441 cctttacccc taatcctgac agtgaaattg cccactatgt ggcgcagatt cactctacac
     7501 gacagacaaa ggagctttgc gcatgaccac tcaactggaa caggcctggg agctggcgaa
     7561 acagcgtttc gcggcggtgg ggattgatgt cgaggaggcg ctgcgccaac ttgatcgttt
     7621 acccgtttca atgcactgct ggcagggcga tgatgtttcc ggttttgaaa acccggaagg
     7681 ttcgctgacc ggtgggattc aagctactgg taattatccg ggcaaagcgc gtaatgccag
     7741 tgagctacgt accgatctgg aacaggctat gcggctgatt ccggggccga aacggcttaa
     7801 tttacatgcc atctatctgg aatcagatac gccagtctcg cgcgatcaga tcaaaccaga
     7861 gcacttcaaa aactgggttg aatgggcgaa agccaatcag ctcggtctgg attttaaccc
     7921 ctcctgcttt tcgcatccgc taagcgccga tggctttacg ctttcccatg ccgacgacag
     7981 cattcgccag ttctggattg atcactgcaa agccagccgt cgcgtttcgg cctattttgg
     8041 cgagcaactc ggcacaccat cggtgatgaa catctggatc ccggatggta tgaaagatat
     8101 caccgttgac cgtctcgccc cgcgtcagcg tctgctggca gcactggatg aggtgatcag
     8161 cgagaagcta aaccctgcgc accatatcga cgccgttgag agcaaattgt ttggcattgg
     8221 cgcagagagc tacacggttg gctccaatga gttttacatg gggtatgcca ccagccgcca
     8281 gactgcgctg tgcctggacg ccgggcactt ccacccgact gaagtgattt ccgacaagat
     8341 ttccgccgcc atgctgtatg tgccgcagtt gctgctgcac gtcagccgtc cggttcgctg
     8401 ggacagcgat cacgtagtgc tgctggatga tgaaacccag gcaattgcca gtgagattgt
     8461 gcgtcacgat ctgtttgacc gggtgcatat cggccttgac ttcttcgatg cctctatcaa
     8521 ccgcattgcc gcgtgggtca ttggtacacg caatatgaaa aaagccctgc tgcgtgcgtt
     8581 gctggaacct accgccgagc tgcgcaagtt ggaagcggcg ggcgattaca ctgcgcgtct
     8641 ggcactgctg gaagagcaga aatcgttgcc gtggcaggcg gtctgggaaa tgtattgcca
     8701 acgtcacgat accccaacgg gtagcgaatg gctggagagc gtgcgggctt atgagaaagc
     8761 gattttgagc cagcgtgggt aaacactgcc ggatgcggca cgagcgcctt atccggccta
     8821 cgggtcgaat gtccatgcgg gctgtcaccc tcaccccggc cctctccctg aaagggcgag
     8881 ggagaaaaac atagcctcgt gcggcaacgg agctgaacac tgctccggac agtcccctcg
     8941 cccctacggg gagagggtta gggtgagggg aacaagccgc accatgtcta aacaaggcaa
     9001 cctaatttac aggaacacag aacatgcaaa acattaccca gtcctggttt gtccagggaa
     9061 tgatcaaagc caccaccgat gcctggctga aaggctggga cgagcgcaac ggcggcaacc
     9121 tgacgctgcg cctggatgac gccgatatcg cgccttataa agacaacttc catgcacaac
     9181 cgcgttatat cccgctcagc caacccatgc ctttactggc aaatacaccg tttattgtca
     9241 ccggctccgg caaattcttc cgtaacgtcc agcttgatcc tgcagctaac ttaggcgtcg
     9301 taaaagtcga cagcgacggc gcgggctacc atattctctg ggggttaacc aacgaagccg
     9361 ttccaacttc tgaacttccg gctcactttc tctcccactg cgagcgcatt aaagccacca
     9421 acggtaaaga tcgggtgatc atgcactgcc acgccaccaa cctgatcgcc ctcacctatg
     9481 tactggaaaa cgacaccgcg gtctttactc gccaactgtg ggaaggcagc accgagtgtc
     9541 tggtggtatt cccggatggc gttggcattc tgccgtggat ggtgcccggc acggacgaaa
     9601 tcggccaggc gaccgcacaa gagatgcaaa aacattcgct ggtgttgtgg cccttccacg
     9661 gcgtcttcgg cagcgggtcg acgttggatg aaacattcgg tttaatcgac accgcagaaa
     9721 aatcagcaca agtattagtg aaggtttatt cgatgggcgg catgaaacag accatcagcc
     9781 gtgaagagtt gatagcgctc ggcaagcgct tcggcgttac accactagcc agtgcgctgg
     9841 cgctgtaagg agcaaacatg atccgcaaag cctttgtcat gcaggttaac cccgacgccc
     9901 acgaagagta tcagcgtcgg cataacccca tctggccgga actggaagca gtgttgaaat
     9961 ctcacggtgc gcataactac gccatttacc tcgacaaagc gcgtaatctg ctgtttgcca
    10021 cggtagagat tgaatctgaa gaacggtgga atgcggtagc cagtactgat gtttgccaac
    10081 gttggtggaa atatatgacc gatgttatgc ccgctaaccc ggataacagc ccggtgagta
    10141 gcgagctgca agaagtgttt tacctgccgt aattgcccaa tgtgccggag atgcctccgg
    10201 cacaatattt taaatttcga tttgaaaaat taaacatatt acatctcccg gtaagttata
    10261 tttccctgat acattgtgag taaatcacaa aaataatgaa taacccatta atgattcatg
    10321 tggtttattt aaataaccat tatgtgcatt actccgcaaa tctgaccttt cactctgtct
    10381 caaattgtca ttgtttaccg acatatcgcc tctcatcact taatcgctcc accattacaa
    10441 ttgattaaaa tatattttat ggagtccgat taatggcagc tcttactgca agctgtattg
    10501 acctgaatat tcagggcaat ggcgcttatt ccgttctgaa gcagttggcg acaatagcgt
    10561 tacaaaacgg ttttatcacc gactcacacc agttcctgca aaccctgctg ctgcgcgaaa
    10621 aaatgcactc tactggattt ggttccggtg tcgccgtgcc gcacggtaaa agcgcctgcg
    10681 ttaaacaacc gttcgtatta ttcgcccgca aagcgcaggc tattgactgg aaagccagcg
    10741 atggcgaaga cgtcaattgc tggatctgcc tcggcgtgcc gcaaagcggc gaagaggatc
    10801 aggtcaaaat catcggcaca ctgtgtcgca aaattatcca ccaggatttt attcatcaac
    10861 tgcaacaggg cgacgccgac caggtgcttg ccttgttaaa tcaaaccctc agctcataag
    10921 gaagtggcga tggagtcatc cttacgtatt gtcgcgatca ccaactgccc cgccgggatc
    10981 gctcacacct acatggtggc ggaagccctg gaacagaaag cccgttctct cggccatacc
    11041 ataaaagtgg aaactcaagg gtccagtggc gttgaaaacc gcttatccag cgaagagatt
    11101 gccgctgccg attacgtcat tctcgctacc gggcgtggcc tgagcggtga tgatcgcgcg
    11161 cggtttgccg ggaagaaagt ttatgagatt gccatctccc aggcgttgaa aaatatcgac
    11221 cagattttca gcgaattacc gacaaactcg cagctttttg ccgcagatag cggcgtgaag
    11281 cttggcaaac aggaagtgca aagcggcagc gtgatgagcc acctgatggc gggcgtctct
    11341 gcggcgctgc cgtttgtcat cggcggcggt attctggtgg cgcttgccaa catgctggtg
    11401 cagttcggtt tgccatatac cgatatgtcg aaaggtgcgc cgtcgtttac ctgggtagtt
    11461 gaatccatcg gttacctcgg cttcaccttt atgatcccca tcatgggcgc ttatatcgcc
    11521 tcgtcgattg ccgataaacc ggctttcgct ccggcgtttc tggtttgcta tctggcgaac
    11581 gacaaagccc tgctcggcac ccagtcgggt gcgggattcc tcggcgcggt ggtgctgggg
    11641 ctggcgattg gctatttcgt cttctggttc cgcaaagtgc gccttggcaa agcattacaa
    11701 ccgctgctcg gttcaatgct gatcccgttc gttactctgc tggttttcgg cgtgctcact
    11761 tactacgtta tcgggccggt gatgtccgac ctcatgggcg ggctgctcca cttcctgaat
    11821 accattcctc catcaatgaa gtttgcggca gcgtttctgg tcggtgcgat gctggcgttc
    11881 gatatgggcg gccccatcaa taaaaccgcc tggttcttct gcttctcact gctggaaaaa
    11941 cacatttatg actggtacgc catcgtcggc gttgtcgcgt tgatgccgcc cgttgcggcg
    12001 ggtcttgcga cgttcattgc gcccaaactg tttacccgac aggaaaaaga ggctgccagt
    12061 agtgccattg tggtcggtgc cacagtcgca accgaaccgg ctatacctta cgctctggca
    12121 gctccgttgc cgatgattac cgccaatacg ctggcgggtg gcattaccgg cgtgctggtg
    12181 atcgctttcg gaatcaaacg tctggcacct ggtttgggta tctttgaccc gctgatcggc
    12241 ctgatgtcgc cggtgggttc gttttatctg gtgctggcga ttggcctcgc gctgaacatt
    12301 tcattcatta tcgtcctgaa aggattgtgg ttacgccgca aagcgaaagc cgcgcaacag
    12361 gagcttgtcc atgaacattg agttactgca acagttgtgc gaagccagcg ccgtcagcgg
    12421 cgatgaacag gaagttcgcg acattctgat aaacacgctg gaaccttgcg ttaatgaaat
    12481 cacctttgat ggtctgggca gctttgttgc ccgtaagggg aataaaggtc caaaagttgc
    12541 cgttgtcggg catatggatg aagtcggctt tatggtcacc cacatcgacg agagcggttt
    12601 tctgcgcttc accaccattg gcggctggtg gaatcagtcg atgctcaacc accgggtaac
    12661 catacgcaca cacaagggat ttaaaatccc tggtgtgatt ggttccgtcg cgcctcatgc
    12721 gttaacggaa aagcaaaagc aacaaccgct gtcatttgat gagatgttca ttgatattgg
    12781 cgcgaacagt cgcgaagaag cggaaaagcg cggcgttgaa attggcgatt ttattagccc
    12841 ggaagccaat tttgcctgct ggggcgaaga taaagtagtc ggaaaggcgc tggataaccg
    12901 catcggctgc gcgatgatgg cggagctact acagacagta aataacccag aaattacgct
    12961 gtacggcgtt ggcagtgtgg aagaagaagt tgggctacgc ggggcacaaa cctcggcgga
    13021 acacattaaa ccggatgtgg tgattgtgct ggataccgct gtcgcaggtg atgttccggg
    13081 cattgataac attaaatacc cgctgaaact gggccagggg ccggggctga tgctgtttga
    13141 caagcgctac ttccccaacc agaaactggt agcagtgtta aaaaactgtg ccgcacataa
    13201 cgatttaccg ctgcaatttt ccaccatgaa aaccggagcg acggatggcg ggcgctacaa
    13261 cgtgatgggc ggcgggcgtc cggttgtcgc gctgtgtctg ccaactcgtt atctgcacgc
    13321 caacagcggt atgatttcaa aagccgatta cgatgctctg ctcacgctga tacggggttt
    13381 tctgacgacc ttaactgcgg agaaagtcaa cgcgtttagc cagttccgtc aggtggatta
    13441 atgctaaacg aacgccagtt aaagattgtc gatctgctgg agcaacagcc gcgcacgcct
    13501 ggagagctgg cgcaacagac tggcgtttca ggcaggacca tcctgcgtga tattgactat
    13561 ctcaacttca cccttaacgg caaagcccgc attttcgcca gtggtagtgc gggctatcag
    13621 ctggaaatct tcgagcgccg cagctttttt cagttgctgc aaaagcacga taacgacgat
    13681 cggctgctgg cactgttatt actgaatacc tttactcccc gtgcgcaact cgcctcggcg
    13741 cttaatttgc cagaaacgtg ggtcgcagag cgtctgcccc ggttaaaaca gcgttatgaa
    13801 cgcgcttgtt gcctggccag ccgccccggt ttgggccatt tcattgatga gacagaagag
    13861 aagcgcgtta tcttgctggc gaacttgctg cgcaaagatc cgtttttaat tccgctggcg
    13921 ggcgtaacac gagacaacct tcagcattta tccacggcct gcgacaacca acaccgctgg
    13981 ccgctgatgc agggtgatta tctctccagc ctgattctgg cgatttacgc cctgcgtaat
    14041 cagcttactg acatatggcc gcaatatccc ggtaacgaga taaaacaaat cgttgaacag
    14101 tgcggcctgt ttctcggtga taacgctgta agaaccctga cgggtttgat agagaaacag
    14161 catcagcaag cgcaggtaat ttcagccgat catgtgctgg ggttgctgca aagggtgcca
    14221 ggcatcgcgt cattgaatat tattgatacg cagttggttg agaatattac cgggcattta
    14281 ttacgttgcc ttgccgcacc ggtgtggatt gctgagcacc gccaaagcag catgaataac
    14341 ctgaaagccg cctggcctgc ggcctttgat atgagtctgc actttattac gctactgcgt
    14401 gaacagctcg atattcccct tttcgacagc gatctgatcg gtttgtattt tgcctgtgcg
    14461 ctggaacggc atcaaaacga acgccagccg atcattttgc tctcggacca gaacgcgatt
    14521 gccactatta atcagctcgc cattgagcgc gatgttttac attgtcgggt gattattgcc
    14581 cgtagcttaa gcgaacttgt tgccattcgc gaagagattg agccgttatt gatcattaac
    14641 aacagccatt atttactgga tgacgcggta aataattaca tcaccgtaaa aaatatcatt
    14701 acggctgccg gtatcgaaca aataaaacat tttctggcga cggcatttat tcgccaacag
    14761 ccggagcgtt ttttctctgc ccccggaagt tttcattatt cgaatgtacg cggtgaaagc
    14821 tggcaacata ttacccggca aatttgtgcg caattagtgg cacaacacca tattaccgcc
    14881 gatgaagcac aacgcatcat cgcccgcgaa ggcgaaggtg aaaacctgat tgttaatcgc
    14941 ctcgccatcc cacattgctg gagcgaacag gagcgacgtt ttcgtggatt ttttattacc
    15001 ctcgcccaac cagttgaggt gaataacgaa gtcattaacc atgtcttgat cgcctgcgcc
    15061 gccgccgatg cgcgtcatga gctaaaaata tttagctatc tggcaagcgt attgtgtcag
    15121 catccggcag aggttattgc cgggttaaca ggatatgagg catttatgga gttacttcac
    15181 aaggggtgaa ctgtgtcgga tgcgccgcat ccgacactgc atttatcatc aaaaacgttt
    15241 aacgcaggct attatagtca tcaaccattt cgttgtactc gcgtaatgct ctcgggaaag
    15301 agtcgtcgac catccagctt gagtttgcgt cctgcagttg ctctttatcc cagtcagagt
    15361 acgggacttt atctctgacg cggcgaacgt attttttagc ctcgagttga tactggcctg
    15421 ccgaattaat aaacgagaaa ttcatgccgc ctttatccgc ttctttggcc tgcgccacca
    15481 gcgtttccag ttcagacact ttcttcattg ccgcttctgc atcaaactta tcttgcgata
    15541 tcaggttatt aatttgtttc gctgaaatca tgactgccag agagtaatag tggaatgttt
    15601 tcccttctct ttcttcaata tttttcagtt ccgcaagctg gcgcttatcg ttaatttcct
    15661 gaatcgccgc atgataagat tctgctacag cttcaaaggc ttccagattt tttaagaatg
    15721 tctggtgcag cgttttccct ttcgcaaacg catcgtcttt ataattttcc tgggtgtaat
    15781 atttatccat ttcgttaatg gtattaccca acgccacggc agcatcgata taactcaccg
    15841 ccacgccatc aataggttgc agcgcaggcg ttaacgccac agcacttttt acgccttttt
    15901 cacactcagc gaggttggat tcactaatgc cgtagatgcc ataaacagta cgctcttcac
    15961 cggtaggccc ctgtttaaaa tctttcagcc agtcagcata acgcgccaga ctgctctgta
    16021 cagggatctg taacttgtta tagcacttga tatagacatc cattttttcg tcgaaaaccg
    16081 cttgttcatc gaccaccggt tgcgcaggtg tttcaggttt agcttctgct tttgcaacgg
    16141 gagcttcagg agctggtgcg gcaggttgac tattggcagg cgggggcgtt tctgtttcgg
    16201 cttttttatc atcacaaccc gtcagaccga gggccatgat ggcgactata atagcggaag
    16261 ataataaatt ccttttcatt taattgcctt ataaatatat agataagaga gtgaacataa
    16321 taaatgaaaa aagaaaacgc cactacacgc gttttattta taaataaaat aataaaattc
    16381 acgaaatgaa taagtcctgc aatttaatat atatcgcagg acttatttta attaattgct
    16441 caaacgctgc ggatgggtat aaaccgttgc ccgacccggc ttacaaaaac ccaccagcgt
    16501 cagattacaa cgctcagcca cttctacagc aagcgtggtc gcggcagaca ccgcaaacaa
    16561 aatctctacg ccgcacatcg ccgacttttg caccatttca taactggcac gactggaaac
    16621 cagtaccgca ccttgctgcc agctttcccc ttcttgtgac cggcggccta acagtttgtc
    16681 cagcgccaca tggcgaccca cgtcttcatg cccgccgacc agttcgccag atggcaacat
    16741 ccaggcagcg gcgtgagtac aaccagtcag ttgccccact ggctgaaaat cgttgagatg
    16801 acgtaatgca tcatccagtt tgttgagatc aaacgtctgg gtgaacggta gcggctgcac
    16861 cggttttccg atgtcattaa gttgctccac gccgcatacg ccacagcccg tacgtcccgc
    16921 cagcgcccgg cggcgctcct tcaaccccat aaagcggcgg ctggaaagct caatttgtac
    16981 ttcaagacca ttacaggaag gaacgacatc catgccgaag atatcgcgcg gactttctat
    17041 tatcccctcg gaaagcgaaa aaccgagcgc aaagtactca aggtctttgg gcgacgccat
    17101 catcaccaca tgcgaaatgc cgttgtagac cagcgcaacg ggaacttctt ccgcgacctc
    17161 atctaaccgt gggtgttgta aatcatcacg ccgccataac tcaatttgac gaacacctgt
    17221 gatatttgtc acattttcga tttctttttg ctgtgttttt ttcactttct cttaaccaga
    17281 taagaacaca cacaccaaca ttatggtatt ctgttacaaa cccttcctgg atggagggaa
    17341 attgagccaa ttctggacct ttgcggcccc ttccgcaaag aaaaataact cccactccct
    17401 gcacacgcag caagcgaatg taaatgggac gtgacaatgt cgaaacaagg agcaatccat
    17461 gcaggtcagc agaaggcagt tctttaagat ctgcgctggc ggtatggcag gcaccacggc
    17521 agcggcactg ggttttgcac ccagcgtagc gctcgcggaa acccggcagt ataaactgct
    17581 gcgcacccgc gaaacccgta atacctgcac ctattgttcc gtaggctgtg ggctgttgat
    17641 gtacagcctc ggtgacggag caaaaaacgc caaagcatct atcttccata tcgaaggcga
    17701 tccggatcac ccggtcaacc gcggtgcact ttgtccgaaa ggcgctggcc tggtggattt
    17761 catccactct gaaagccgtc tgaagtttcc ggaataccgt gcgccaggtt ctgataaatg
    17821 gcagcaaatc agttgggaag aggcgtttga tcgcatcgcc aaactgatga aagaagaccg
    17881 cgatgctaac tacattgcgc aaaacgccga aggcgtgact gttaaccgct ggctctccac
    17941 cgggatgctg tgtgcttccg cgtcgagtaa cgaaaccggc tatttaacgc aaaaattctc
    18001 ccgcgcgctg ggtatgctcg cggtcgacaa ccaggcgcgt gtctgacacg gaccaacggt
    18061 agcaagtctt gctccaacat ttggtcgcgg tgcgatgacc aaccactggg tcgacatcaa
    18121 gaacgccaac ctcgtcgtgg tgatgggcgg taacgccgct gaagctcacc cggtcgggtt
    18181 ccgctgggcg atggaagcca aaattcacaa cggcgcgaag ctgattgtga tcgatcctcg
    18241 ctttacgcgt acggcggcgg tagctgacta ctatgcccct attcgttccg gtactgacat
    18301 tgctttcctg tcaggcgtat tgctgtacct gctgaacaat gaaaaattca accgcgaata
    18361 caccgaagcc tataccaacg ccagcctgat agtgcgtgag gattacggct ttgaagatgg
    18421 cctgttcacc ggctacgacg cggaaaaacg caagtacgat aaatcctcct ggacttatga
    18481 actggacgaa aacggcttcg ccaaacgcga taccacgctg caacatccgc gctgcgtgtg
    18541 gaacttgctg aaacagcacg tttcccgcta cacgccagat gtggttgaaa acatctgtgg
    18601 tacgccaaaa gacgcgttcc tgaaagtctg cgaatacatc gcagaaacca gtgctcacga
    18661 taaaactgcc tcgttcctgt atgccctcgg ctggacgcaa cactccgttg gtgcgcaaaa
    18721 cattcgtacg atggcgatga tccagctgct gctcggcaat atggggatgg ccggcggcgg
    18781 cgttaacgcc ctgcgcggtc actccaatat tcaggggctg acggacctgg ggctactgtc
    18841 gcagagtctg ccaggttaca tgacgctgcc aagcgagaag cagaccgatc tgcaaaccta
    18901 ccttaccgcc aacacgccaa aaccgctgct ggaaggccag gtaaactact ggggcaacta
    18961 cccgaaattc ttcgtctcta tgatgaaggc cttctttggt gataaagcga cggcggaaaa
    19021 tagctggggc tttgactggt tgccgaagtg ggataaaggc tacgacgtcc tgcagtactt
    19081 cgagatgatg aaagagggca aggtcaatgg ctatatctgc cagggcttta accctgttgc
    19141 ctcattcccg aacaaaaaca aagtgatcgg ttgtctgtcg aaactgaagt tcctcgtcac
    19201 catcgaccca ctgaacactg aaacctctaa cttctggcag aaccacggtg agctgaacga
    19261 agttgactcg tcgaagatcc agaccgaagt gttccgtctg ccatcgacct gcttcgcaga
    19321 agagaacggt tcaatcgtta actcaggtcg ctggttgcag tggcactgga aaggtgcgga
    19381 cgccccgggg attgcgctga ctgatggcga gatcctctcc ggtatcttcc tgcgcttgcg
    19441 caagatgtat gccgaacagg gtggcgcgaa cccggaccag gtgctgaaca tgacctggaa
    19501 ctacgccatt ccgcatgagc cgaagtcgga agaagtagcg atggagagca acggtaaggc
    19561 gctggccgat attaccgatc cggcaaccgg tgcggttatc gtcaagaaag gccaacaact
    19621 tagctcgttc gcccaattgc gcgatgacgg tacaacctcc tgtggctgct ggattttcgc
    19681 cggtagctgg acgccggaag gcaaccagat ggcgcgtcgt gataacgccg atccgtctgg
    19741 cctcggtaac acgctgggct gggcatgggc atggccgctt aaccgccgca ttctgtataa
    19801 ccgcgcctcc gcagatccgc agggtaaccc gtgggatccg aagcgtcagt tgctgaaatg
    19861 ggacggcact aagtggaccg gctgggatat tccggactac agcgcagcgc ctccgggtag
    19921 cggcgtcggg ccgtttatca tgcagcagga aggcatgggg cgtctgtttg ccctcgataa
    19981 gatggcggaa ggtccgttcc cggaacacta cgagccgttt gaaacgccgc tgggaactaa
    20041 cccgctgcat ccaaacgtta tctcgaaccc ggctgcacgt atctttaaag acgacgccga
    20101 agcattgggt aaagccgata agttcccgta tgtcggaacc acctatcgtc tgaccgagca
    20161 cttccactac tggaccaaac acgcgctgtt gaacgcgatt ttgcaaccag agcagtttgt
    20221 ggaaatcggg gagtcgctgg cgaataaact tggcattgcc cagggcgata ccgtgaaagt
    20281 ctcctccaac cgtggctata tcaaagccaa agcggtggtg accaaacgta ttcgtacgct
    20341 gaaagcgaac ggcaaagata tcgataccat cggtattccg attcactggg gttatgaagg
    20401 cgtagctaaa aaaggcttta ttgccaatac gttaacgcca ttcgttggtg atgcgaacac
    20461 gcagacgccg gagtttaagt ccttccttgt gaatgtggaa aaggtgtaac ggagacgaca
    20521 tatggcttat caatctcaag atatcattcg tcgttccgcg actaacggtc tgacccccgc
    20581 gcctcaggcg cgggacttcc aggaagaagt ggcgaaactc atcgacgtca ccacctgtat
    20641 cggctgtaaa gcctgtcagg tggcgtgttc agagtggaac gacattcgcg ataccgtcgg
    20701 caataatatt ggggtgtacg acaaccccaa tgatttaagc gccaaatcgt ggacggtgat
    20761 gcgcttctcg gaagtggagc agaacgacaa actggaatgg ctgatccgca aagacggctg
    20821 tatgcactgt tccgatccag gctgcctgaa agcgtgcccg gcggaaggtg caattattca
    20881 gtatgccaac ggtatcgtcg acttccagtc cgagcagtgc attggctgcg gttattgcat
    20941 tgcgggctgt ccgttcgaca ttccgcgcct caacccggaa gacaaccgcg tctacaaatg
    21001 tacgctgtgc gttgaccgcg tggtggttgg gcaagaacca gcctgcgtga agacctgccc
    21061 aacgggcgcg attcacttcg gtacgaaaga gtcgatgaaa acgctggcga gcgagcgcgt
    21121 tgctgagctg aaaacccgcg gttacgacaa tgcgggtctg tacgatccgg caggcgtcgg
    21181 tggtacacac gtcatgtacg tgctgcacca tgctgacaag ccaaatctgt atcatggctt
    21241 gccggagaac ccggaaatca gcgaaaccgt gaaattctgg aaaggcatct ggaaaccgct
    21301 cgcggctgtt ggctttgcgg ctacctttgc ggccagtatc ttccactacg tgggtgtcgg
    21361 tccgaaccgt gcggatgagg aagagaataa tctgcacgaa gagaaagacg aggagcgcaa
    21421 atgaaacgac gtgacaccat cgtgcgctac accgcgccgg aacgtatcaa ccactggatc
    21481 accgccttct gcttcatcct ggcggcggtg agcgggctgg gctttttgtt cccgtccttc
    21541 aactggttga tgcaaatcat gggcacaccg cagctggcgc gaattctgca cccgtttgtc
    21601 ggcgtggtta tgtttgcctc gttcatcatc atgtttttcc gttactggca tcacaaccta
    21661 atcaatcggg atgatatctt ttgggcgaag aatattcgta agatcgtcgt caacgaggaa
    21721 gtaggtgaca ccgggcgtta taacttcggt cagaaatgcg ttttctgggc ggcgattatt
    21781 ttcctggttc tgctgctggt gagcggcgtg attatctggc gtccttattt tgcgcctgct
    21841 ttctcaatcc cggtgatccg attcgcgtta atgctgcatt catttgccgc agtggcgtta
    21901 attgtggtta tcatggtgca tatctacgcc gccctttggg tgaaaggcac gattaccgcc
    21961 atggtggaag gatgggttac cagcgcctgg gcgaagaaac atcacccgcg ctggtaccgt
    22021 gaggtccgca agacaacgga aaagaaagct gaatgagtat tcgcataatc ccgcaagatg
    22081 agctgggttc gagcgagaaa cgtacggcgg atatgattcc gccgttattg ttccctcggc
    22141 tcaagaattt atacaaccgc cgcgccgaac gtctgcgcga gctggcagaa aataatccgc
    22201 tgggtgatta cctgcgcttt gctgcgctta tcgcccacgc ccaggaagtg gtgctgtacg
    22261 accatccgct ggagatggat ctgaccgcgc gcattaaaga agccagcgca cagggcaagc
    22321 ccccgctgga tattcacgtt ctgccgcgtg ataagcactg gcaaaagctg ctgatggcgc
    22381 tgattgctga gctgaaacct gaaatgagcg gcccggcgct ggcagtgatt gagaatctgg
    22441 agaaggcatc gactcaggag ctggaagata tggccagcgc actgtttgcc tctgatttct
    22501 catccgtcag cagcgataaa gcgccgttta tctgggctgc actgtcgctc tactgggcgc
    22561 agatggccaa tctgatcccc ggcaaagccc gcgctgaata cggcgagcaa cgtcaatatt
    22621 gcccggtttg tggttctatg ccggtgtcca gcatggtgca aattggcacc actcagggtc
    22681 tgcgttacct gcactgcaac ctgtgtgaaa ccgaatggca cgtagtgcgc gtaaaatgca
    22741 gcaactgcga gcagagcggc aaactgcatt actggtcgct ggatgacgaa caggccgcga
    22801 ttaaagccga aagctgcgat gactgcggca cttacctgaa gattctttat caggaaaaag
    22861 aaccgaaagt tgaagccgtg gcagatgacc tcgcttctct ggtactggac gcgcgaatgg
    22921 agcaagaagg ctatgcccgc agttccatca acccgttcct gtttccgggt gaaggggagt
    22981 aatctctgac gttttccggg tggcgctggt cgcccggatt acagctcata gggtgtgacg
    23041 acactatctc tcgtattccg cgttaccacc tcaagctatg ccgccagtgc cggtttggcc
    23101 tcaatttacc caccaccaca tcaataaggt tatgggcatg agactgatga caagcgtaag
    23161 attgcctgat gcgctgcgct tatcaggcct accaataaac tgcaatacat tgaatttata
    23221 cgactttgta ggccggataa ggcgttcacg ccgcatccgg catgaacaac gcgcacgttg
    23281 tcagcaatct gattgacatc attttttccc attaccattc ctcgcgcagc ttacgctgat
    23341 attcgacacc atcttcctca cacccttgcc agatgccagg aacactggtt cttgctgttt
    23401 gcgattgatt tatcaggtat tgatctaccg cttcccttaa taggtccgca cgaggaagat
    23461 tacgctgcac ctcgagatca tcaagttgct taatcacctc attcgataaa tcgagtaaaa
    23521 ttctgcccat atctatacct gccagagagt tcataaatat ctcgccaata tcatttttga
    23581 atctatggag agaaaagtac ccttgccgaa tctttaaaga aagctcattt acgcatcatt
    23641 ttttattttt gcgaggcgtt ttccagatat gatgtaacag ttgcagttat tataagttca
    23701 agtaaaacag ccttgtatta ataccagaat aagggcatct gttttttata ttcaagaatg
    23761 aaaaattttt gtcattcctt atgctcctta cactgatcat caacaccaac tacctccccc
    23821 ttttgcctgc ctccctttgc tttacgcacc gggaattgct gacgttccag atattgctct
    23881 accgcttcgc gtaaaattgc agatcgtgaa accttacgcc agccccgaag cttgttcagc
    23941 cgtttaattg cctcttctga taaatgaaga aaaaccgtat tcatcgccat taccaccacc
    24001 ttacctatat tactcattaa tgtatgtgcg aaaattacgc cggaaaatca tttaaaaaaa
    24061 gcggattttt gcatgttgtt ttgtttttgc gaggcgtttt ccagaaataa taagagtgtt
    24121 gcagttgttt catttattac tcaaaaatgg tttagttatc gcccaggatg aattatgggt
    24181 gctatgaata tattatattc agagatattt atttgcataa tctgaatggt gtagccgaaa
    24241 aacgtcagca tcctaacagc accaggattt aaggtgaaat taatctttca tcacagaccg
    24301 gtcagtactg ccaccaaccg gtctttactt gctcatcgtt ataattttgt tggtaacagt
    24361 tcgggaaagt tgtttttcta aattaacaac agaataactt tcttgtttaa tgagattaat
    24421 attggcaacc attatagcct ccttcccacg gtttaagagc aaacatacaa cataattatt
    24481 cataacatac agagcagtaa atgaatgata ctgacaatga tgctagtcca ttatctttgc
    24541 ataatatttg ttagctatag attttaaaaa tatttatgag aaaagtatgg caccagaggg
    24601 ggttttgact atcaaaaatc gctcaaacct gaactaagat gatgagttaa gcattatctg
    24661 cgataattgc cactcagctc tacaaataat atttaaatgc tcctgaacag tgatgtagca
    24721 agacacgtat ggagccaacg aagaataaaa atttatagag ttcttattca atataaccgg
    24781 aatagtcagg ggctgatgta aatgaccgga atgagtaaga gaggcagcac cgcccctctc
    24841 catcagctac tcttcttcgt tcccgccctc ttcatacggg ccaaatggct tcgcgggcag
    24901 aacgatatac gtgccttcaa acactgcacc cggcgtctcg tcgccaaaga tttcaacttg
    24961 catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg agatcgccgc ttaaggcacc
    25021 aaggtcggct accgcatgag gtttaccgct aatcggcttg ctgtagcgga tatgcgcatc
    25081 cgccagaata atcgttccgc cgagatggcg ttcgcgcagc atcagccaga taagccccca
    25141 accggtgagc gtcgccagcg agaataaact cccggcaaac agcgtatggt gcggattctg
    25201 attgccggtt tccggcatgg tggtgataaa tttttgcccg gtatattgtt gaatacgcac
    25261 gcccattttt tcactaagcg ggatatgttc gtaccacgcc tgttgcagct gcgcgcacca
    25321 gtcgccgcga tgcaaaatat catccagagt ggcgacgggc ttaatcatca aaaaatggcg
    25381 aatcggcgtg gtggttggcg tggtgatttc tccctgatta acaaacccca gcttggcgaa
    25441 aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc ttaacgcctt cctgacgcgc
    25501 caccgactcc agggtcatcg ccatcagcgt tcctaaccct ttgtcctgca cgtccgggtg
    25561 aacggccata aagcgaatgg acgcttcatt gtcggcatta atatacagtc ggcctaccgc
    25621 caccagatta ccctgctcgt cgacgaccat ctggtgatgc gccatcgcat cccacgcgtc
    25681 gcgttccgaa ccttttggtt gatgcagggg cttacgcaac atttcccagc gaaactgata
    25741 gtaacgctct aattcttctt ctgtttgtgg aacccgaagg tgatacatag cgatactctc
    25801 tcttgtttcc cgtgacaacc ctggaagctg gctcatacct gcaaccagaa tgtcacgggg
    25861 ccatcattga ccagcgatac ctgcatatcg gcagcgaagc gtcctgtttg cgtgttcatc
    25921 tcttgctgac ggcagcgttc gacgaaatag tcatataacg cctctgcgcg atccggtgat
    25981 gcacctttgg agaaacttgg gcgcatcccc cgttcggtat ctgcggcgag ggtaaactgg
    26041 gaaaccacca gcacactgcc gcccgcctgt tgcacgttga ggttcatctt gccttcggca
    26101 tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac ggtttgcttt ctgttcgtcg
    26161 tcatcctttt cgacacccaa taacaccaaa agtcccgcgc caatttcgcc cgtcacttct
    26221 ccctccacgg tgacgctggc acgggttacg cgttgaatta atgcaatcat ggttcgtcgt
    26281 cttcttcttg ttcagctgct tgttttagtt tgcggtattc cccgagagtg acagtaattt
    26341 ccgcgccaag caagacgata caccatgtcc agtagaccca aacaaagaga atggggatca
    26401 ccgccaacac gccgtaaatg agctgatatg acggaaacat ggtgatataa agcgcgaaac
    26461 ctttctttcc tgcttcgaac aggagcgcgg cgacaaacgc gccgacaatc gcgtcgcggt
    26521 taggtacgcg gatggtagga acaatgctgt acagcaacca gaaggagatc cacgacaaca
    26581 gcagcggaaa aatacgcagc acgttatcga tgacagtatt gagatcgctc gcccagcgca
    26641 gggagagcaa ataggaactg atcgccagac tggcccctgc cagcagcggc cccagcgtta
    26701 aaatcatcca gtacacggcg aacgagtaaa tttttggtcg cgctcgttta ctgcgccaga
    26761 tggtattcaa cgcgctatcg atggagtaca tcagcaataa cgcggtgacg atcagcccgc
    26821 acgccccaac ggcggtcatc ttgttggaat tggcaacaaa ttgttcgata taccgctgaa
    26881 taacatcgcc agtagcagga agaaagttgg caaaaataaa gtgacgtaac tgaatgctga
    26941 cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac aacggcaacc agcggcacta
    27001 atgagagcaa cgacacatag gcaaggttac ctgccagggt tgtcatgttg tcctcatcaa
    27061 tgcgttgcca gagtagtttt agccaggccc atagtggacg ggtacggtgc ctggctttgt
    27121 cctgaatggt ttttagcata acaccttcgc gaaatagtcc gggatggtgg ttttatcttt
    27181 caccagaata ctggtaatgc ccagctgatt ggctccttct atattatcgg cgttatcgtc
    27241 gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc aaaacatgct ggtaaattcg
    27301 tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag atatggtcag cagcatcacg
    27361 aatttccggg tattcttccg gccagaaggt ggtatgcagg cggttggtat tggaaagcac
    27421 caccacgcga tgcccctgct cacgcagttt atgcatgatg gcgatcactt ccgggcgcag
    27481 cgcaacaaac accgcctgcc agccgtgaga gaactgctcg tagcttagcg gtagagccat
    27541 ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt tccccacgct catgctgatg
    27601 aaacgcctcc cccatatgaa aactcttctt aagcgatgcc agcggaatac gcgttaaatc
    27661 gctccaggct cccagcacac ggttaaagtc gatatcgaca atcacattac ctaaatcaaa
    27721 gatatagagc atttttgcct cctttcgcgc cacaagaaat taactgtagc gggaaaggat
    27781 gggtttggct atgtggcaag gtatgaagcc aggttaacgg tcacaaaaat ctacgcgtct
    27841 tcctggggaa gaatgatttc aatttggctg tcttgtaagc gtgattttag cgcagcgggc
    27901 ggcagctcgt cggtaaagag cgcagtgacc tgtgccacgt taccaatttc aaccgccgcc
    27961 gaagcatgat acttagtgtg atcggcgacc agcagaatat ttctcgcgtg cgccatcatc
    28021 gttttcacca cgttagcttc gtttacatca aactccatca acgcgccatc gctctcaatc
    28081 gccccaacgc ttgttaccag ataatcagca cgaaaatcag ccacaaagga cgccgctgaa
    28141 gggccaatga tcccgctatt atgagagcgc aacgtaccgc cgggcaccat cacttcaaag
    28201 cgcgggttgt ggtaaagaat atgcgccaca cgcaggctgt tggtgattat ccgcaaatga
    28261 ttatggttaa gtaacgcccg ggcaacatgc tcaacagtcg taccaatggt gataaatatt
    28321 gttgaaccat caggaatata gtctgccacc gcttcggcaa tcgctttttt ttcctcggtt
    28381 tgcgaaacct cacgctgctc gaacgccgta ttaacgacgc tggaagcccg acccgcgcca
    28441 ccgtgatggc gcgtaatcag gccttgctcg cttaatttac gaatatcccg gcggaccgtc
    28501 tgcgtggaga catccagcag atttgccagc tcatcaatat tcatataccc acgctcggcg
    28561 atcagcatga ggagttggtc gtgccgcggg ttaccggtta gttcggtaag gctcatgaaa
    28621 attcctcgaa aaaccatcac cctggcattt tatacaaaaa gtgacaaaaa agatcgggtt
    28681 tgatcacagt cagggatccc ggctcgtcca ccgggacgtg tgcattttaa cgccgctaca
    28741 ccgctggcga agcggactga ctccgctaaa tccccacttg ttgccagcgc caccgccaaa
    28801 gcgccgtgaa aaacatcacc cgcacctgtg gtatctacca catcaacttt gaaggccggt
    28861 tgatgctgac gcccaccatt ttccagccag tcgcagcctg cgctaccctg ggtcacatag
    28921 acatgaccat ttgtgagcgt ttgtgcctgt tttagcgcac tggccatctc tttcacgccc
    28981 gttaagcgcg ccagacccgg ttctgaaaag gccgcgtgat cgcttaatgc caccagctca
    29041 ctgatatcct gcggcgtaat gtccccgtcc agaacggtca tcacacccgc ctgacgggcc
    29101 agggtgaagg cttttttagc gccgtcgtgc cagcgtacat ctgccagcac aacatcccac
    29161 tgagagaaat caatttcctc caaccactct gcgtcaggca gcagatccgg gctggggtag
    29221 ttaatgatta tccgctcgcc tttggtatcc accatgatgg cggattgcga agatttcgcc
    29281 tggttatacc gtttggtgta acgggtgtta acgccccagg attccagctc tgccagcagg
    29341 ctgttgcccg tgtcgtcatc acctacgcga ccaataaaat cgacctgcgc ccccagcctt
    29401 gccgccgcaa ccgctgccgt cgccgctggc ccgccaccaa cttccgtata atttctcgcc
    29461 acgtatttac cgctctccgt cggtaaccct tccacgtaat agatgcgatc catcacggtt
    29521 atacctacac aagcaacacg aatcatggtc attccttaaa cattttggga tgcgttcatt
    29581 ttaatttcac caattgttta aaaagtgacg gctgtcaatt ttttgacgat aaattacaaa
    29641 tacgatcaaa aacagacaag caaagcatgt cataaatgac aaaaagtgac atcactgtat
    29701 tcaggagagg gttatggcag caatcgcgtt tatcggttta ggacaaatgg gttcgccaat
    29761 ggcgagcaat ttattgcagc aagggcatca acttcgcgtc tttgatgtga acgccgaagc
    29821 tgtgcggcat ctggtagaca aaggtgcgac tcccgccgcc aacccggcgc aggccgctaa
    29881 agatgccgaa tttatcatta ccatgttgcc gaatggcgat ctggtgcgca acgtgttgtt
    29941 cggtgaaaac ggcgtttgcg aaggcttatc taccgatgcg ctggtcattg atatgtccac
    30001 catccatccg ctgcaaaccg ataaattgat tgccgatatg caagccaaag gcttcagcat
    30061 gatggatgtt ccggtaggcc gtacttctgc aaatgccatt accggtactc tgttactgct
    30121 ggctggcggc accgctgaac aagttgaacg tgccacaccg atcctgatgg cgatgggcag
    30181 tgagttgatc aactctggcg gtccgggcat ggggatccgc gttaagctca tcaacaacta
    30241 catgagcatc gcgctcaatg cgctttcggc agaagccgcc gttttgtgcg aagccctgaa
    30301 tcttcccttc gatgttgccg tcaaagtgat gagcggtacc gccgccggta aaggtcactt
    30361 caccacttcc tggccaaaca aagtcctcag cggagatctt tctcccgcct tcatgatcga
    30421 tcttgcccat aaggatcttg gcatcgccct tgatgtcgcc aaccagctgc atgtgccaat
    30481 gccgctgggg gccgcctcac gggaggttta tagccaggcg cgcgcagcgg gtcgcggtcg
    30541 ccaggactgg tccgccattc tggaacaggt ccgtgtcagt gccgggatga ctgccaaagt
    30601 aaaaatgtaa cgactggata aaggaataaa tgaatgaata agtacaccat caacgacatt
    30661 acgcgcgcat cgggcggttt tgccatgctg gcggtcgatc agcgcgaagc catgcgcatg
    30721 atgtttgccg cggctggggc acccgctccg gtagccgata gcgttttaac tgatttcaaa
    30781 gttaacgctg caaaggccct ctcgccttat gcctcggcga ttctggtaga tcaacaattc
    30841 tgctaccgcc aggtggttga gcaaaacgcg attgccaaaa gttgcgccat gattgtcgcc
    30901 gccgatgagt tcattcctgg caacggtatt ccggtcgata gcgtggttat tgaccgcaaa
    30961 atcaatccgc tacagatcaa acaagacggt ggcaaagcct taaaactgct ggtgctgtgg
    31021 cgtagtgatg aagatgcgca gcaacgtctg gatatggtga aagagttcaa cgaactgtgc
    31081 cactcacacg gtctggtaag catcattgag ccagtcgtcc gcccaccgcg tcgtggcgat
    31141 aaattcgatc gcgaacaagc gatcatcgat gccgccaaag agctgggcga cagtggcgct
    31201 gacctctaca aagttgaaat gccgctttat ggcaaaggtc cgcaacaaga gcttctctgt
    31261 gcttcacaac gtctgaatga ccatatcaat atgccatggg tgatcctctc ttccggtgtc
    31321 gacgaaaaac tgttcccgcg tgccgtacgc gtggcaatga cggcgggcgc atcgggattc
    31381 ctggcaggcc gtgcagtctg ggcatcggtc gtcggtttac cagacaacga gctgatgctg
    31441 cgtgacgttt gcgcaccgaa attacaacaa cttggcgata tcgtcgacga aatgatggct
    31501 aaacgccgct aagatgagga taaaaggaat gaaatggttt aacaccctaa gccacaaccg
    31561 ttggctggaa caggaaaccg accgcatctt tgattttggt aaaaattccg tagtgccgac
    31621 tggttttggc tggttaggca ataaagggca aatcaaagaa gagatgggca cccatctgtg
    31681 gatcaccgct cgtatgttgc acgtttattc cgttgctgcg gcgatgggtc gacctggcgc
    31741 ttactcgttg gttgatcacg gtatcaaagc catgaacggc gcactgcgtg ataaaaaata
    31801 tggcggctgg tatgcctgcg tgaatgacga gggcgtggtg gatgcctcca aacagggcta
    31861 tcaacacttc tttgctctgc tgggtgccgc cagcgccgtc acaacgggtc acccggaagc
    31921 gcgcaagctg ctcgattaca ccattgaaat tatcgagaaa tatttctgga gcgaagaaga
    31981 gcagatgtgc ctggaatcct gggacgaagc cttcagcaaa accgaagagt accgcggcgg
    32041 caatgccaat atgcacgcgg tggaagcttt cttgattgtt tatgacgtca ctcacgacaa
    32101 aaaatggctg gatcgcgcga ttcgcgtggc ttccgtgatt atccacgacg tcgccagaaa
    32161 taatcattat cgcgttaacg aacacttcga tacccagtgg aatccgctgc cggattacaa
    32221 caaagataac ccggcgcatc gcttccgcgc gttcggaggt acaccaggcc actggatcga
    32281 atggggccgt ttaatgctgc acatccatgc tgccctggaa gcccgttgcg aacaaccacc
    32341 agcatggctg ctagaagatg ccaaaggtct gtttaacgcc accgtgcgcg atgcctgggc
    32401 acccgatggc gcggacggga ttgtttatac cgttgactgg gaaggaaaac cggtggtccg
    32461 cgaacgtgta cgttggccta tcgtcgaagc aatgggtacg gcctacgcgc tctacaccgt
    32521 cactggcgat cgccagtatg aaacctggta tcaaacatgg tgggagtact gcattaagta
    32581 cctgatggac tatgaaaatg gttcctggtg gcaggagctg gatgcggaca ataaggtcac
    32641 caccaaagtc tgggacggca aacaggatat ttatcacctg ctgcattgcc tggtgatccc
    32701 gcgtatcccg ttagcgcccg gcatggctcc agcggttgca gcgggtctgc tggatattaa
    32761 tgcgaaataa ctgatctgaa aatagggtgc gggttttccc ctcaccctaa ccctctcccc
    32821 aaaggggtga ggggaccgtc cggtgcagtt tttgggtttg acatcagcct cacgggaggc
    32881 tgtatgtcgt taattaaggt gccttgtatg caaatcacca atatgcattg cagtggacag
    32941 accgtttcac ttgccgcggg ggattatcac gcgaccattg tgaccgtcgg tgctggtctg
    33001 gctgaattaa cctttcaggg ttgtcattta gtcatcccgc acaaacccga agagatgccg
    33061 ctggcgcatc tgggtaaagt gcttattccg tggccgaacc ggatcgccaa cggctgttac
    33121 cgctatcagg ggcaggaata tcatttaccc attaatgaac atagttcaaa agccgctatt
    33181 cacgggctgc ttgcctggcg agactggcaa attagtgaat taaccgccac cagcgttacc
    33241 ctgacagctt ttctgccgcc cagttacggc tatccgttta tgctggcttc acaagttgtt
    33301 tattcgctca acgcacacac gggtctttcc gttgagatcg caagccagaa tatcggcact
    33361 gtcgcagcgc cttatggcgt gggtattcat acttatttaa cctgcaactt aacatctgtt
    33421 gatgaatatc tgttccagct tccggcaaac caggtttatg ccgttgatga acacgtaaat
    33481 ccgacaacgc ttcaccacgt tgatgagctg gatttaaatt tcactcaggc gaaaacaata
    33541 gccgcgacaa aaatcgacca cacctttaaa accgctaatg atctgtggga aataaccatt
    33601 acccatccac aacaggcgct ttcggttagc ctttgttgcg atcaaccctg ggtacaaatt
    33661 tatagcggtg aaaaattaca gcgtcaggga ctggcagttg aacctatgag ttgcccgcct
    33721 aacgccttta attcaggtat cgacttatta ttactggaac caggaaaacc gcaccgcctg
    33781 ttttttaaca tttacggaca gagaaaataa cttgtattga ttagcagtca aaatcattga
    33841 agaacatttc actcgctaaa ggcaacatta tgtatcttca atttattaag ttaaaaacag
    33901 ctcagcttct gtaaagtttt tatatatcag ttttatgaga atcattttac ataagcctgt
    33961 aataacagga aggcaacaat ctcgttctca actctatgga gattagttat ggatacgcca
    34021 cgtccacagt tattagattt tcaatttcat cagaataacg acagttttac cctacatttt
    34081 caacaacgtc ttattttaac ccatagcaaa gataatcctt gtttatggat tggctcaggt
    34141 atagcggata tcgatatgtt ccgcggtaat ttcagcatta aagataaact acaggagaaa
    34201 attgcgctta ccgacgccat cgtcagccag tcaccggatg gttggttaat tcatttcagc
    34261 cgtggttctg acattagcgc cacgctgaat atctctgccg acgatcaggg ccgtttattg
    34321 ctggaactac aaaacgacaa ccttaaccac aaccgtatct ggctgcgcct tgccgctcaa
    34381 ccagaggacc atatctacgg ctgcggcgaa cagttttcct acttcgatct gcgtggcaaa
    34441 ccgttcccgc tatggaccag tgaacaaggc gttggtcgca acaaacaaac ctatgtcacc
    34501 tggcaggccg actgcaaaga aaatgcgggc ggcgactatt actggacttt cttcccacag
    34561 cctacgtttg tcagcacgca gaagtattac tgccatgttg ataacagttg ctatatgaac
    34621 ttcgacttta gtgccccgga ataccatgaa ctggcgctgt gggaagacaa agcaacgctg
    34681 cgttttgaat gtgctgacac atacatttcc ctgctggaaa aattaaccgc cctgctggga
    34741 cgccagccag aactgcccga ctggatttat gacggagtaa cgctcggcat tcagggcggg
    34801 acggaagtgt gccagaagaa actggacacc atgcgtaacg cgggcgtgaa ggtcaacggc
    34861 atctgggcgc aggactggtc cggtattcgt atgacctctt ttggcaaacg cgtgatgtgg
    34921 aactggaagt ggaacagcga aaactacccg caactggatt cacgcattaa gcagtggaat
    34981 caggagggcg tgcagttcct ggcctatatc aacccgtatg ttgccagcga taaagatctc
    35041 tgcgaagaag cggcacaaca cggctatctg gcaaaagatg cctctggcgg tgactatctg
    35101 gtggagtttg gcgagtttta cggcggcgtt gtcgatctca ctaatccaga agcctacgcc
    35161 tggttcaagg aagtgatcaa aaagaacatg attgaactcg gctgcggcgg ctggatggct
    35221 gacttcggcg agtatctgcc caccgacacg tacctgcata acggcgtcag tgccgaaatt
    35281 atgcataacg cctggcctgc gctgtgggcg aagtgtaact acgaagccct tgaagaaacg
    35341 ggcaagctcg gcgagatcct tttctttatg cgcgccggtt ctaccggtag ccagaaatac
    35401 tccaccatga tgtgggcggg cgaccagaac gtcgactgga gtctcgacga tggcctggcg
    35461 tcggttgtcc cggcggcgct gtcgctggca atgaccggac atggcctgca ccacagcgac
    35521 attggcggtt acaccaccct gtttgagatg aagcgcagca aagagctgct gctgcgctgg
    35581 tgcgatttca gcgccttcac gccgatgatg cgcacccacg aaggtaaccg tcctggcgac
    35641 aactggcagt ttgacggcga cgcagaaacc atcgcccatt tcgcccgtat gaccaccgtc
    35701 ttcaccaccc tgaaacctta cctgaaagag gccgtcgcgc tgaatgcgaa gtccggcctg
    35761 ccggttatgc gcccgctgtt cctgcattac gaagacgatg cgcacactta caccctgaaa
    35821 tatcagtacc tgttaggtcg cgacattctg gtcgctccgg tgcatgaaga aggccgtagc
    35881 gactggacgc tctatctgcc ggaggataac tgggtccacg cctggacggg tgaagcgttc
    35941 cggggcgggg aagttaccgt taatgcgccc atcggcaagc cgccggtctt ttatcgcgcc
    36001 gatagcgaat gggcggcact gttcgcgtcg ttaaaaagca tctaatcaca tccgcccgtg
    36061 gggaaactca tgggcaaagg gagaacatcg atgagtcaca tcacaacgga agatccagca
    36121 actttacgcc tgccctttaa agagaaactc tcttacggta ttggcgacct ggcctctaac
    36181 atcctgctgg atatcggtac gctttatctt ttgaagtttt ataccgacgt tctggggctg
    36241 ccaggcacct atggcggcat tatctttttg atttcaaaat tctttactgc gtttaccgat
    36301 atgggtaccg gcattatgtt ggattcccga cgcaagatcg gtccaaaagg taagttccgt
    36361 ccttttattc tgtatgcgtc attcccggtc accttactgg cgatcgccaa ctttgtcggc
    36421 acaccgtttg atgtcaccgg taaaacggtg atggccacta ttctgtttat gctttacgga
    36481 ctgtttttca gcatgatgaa ctgctcctac ggcgcaatgg ttcccgctat caccaaaaac
    36541 cccaacgaac gcgcctcact ggcggcatgg cgtcagggcg gcgctacgct gggcctgctg
    36601 ctgtgcacgg tgggattcgt gccagttatg aatcttatcg aaggtaatca gcaacttggc
    36661 tatatcttcg ccgccacgct gttttcactg tttggcctgc tgtttatgtg gatctgctac
    36721 tcgggcgtga aagagcgtta tgtcgaaacc cagcctgcta atccggcgca aaagccgggc
    36781 ctgctgcaat ctttccgcgc aattgcgggt aaccgcccgc tgttcattct gtgcattgcc
    36841 aacctctgca ctttaggggc gtttaacgtc aagctcgcca tccaggtcta ttacacccag
    36901 tacgtgctta acgatcccat cctgttgtcg tatatgggat ttttcagcat gggctgtatt
    36961 ttcatcggcg tattcctgat gcctgcctca gtcagacgtt ttggcaagaa gaaagtttat
    37021 atcggcggcc tgctgatttg ggtgctgggc gatctgctca actatttctt cggcggcggt
    37081 tcggtcagct tcgtggcgtt ctcctgcctg gcgttctttg gctcagcgtt tgttaacagc
    37141 ctgaactggg cgctggtttc cgacaccgtc gagtacggcg agtggcgcac cggcgtgcgt
    37201 tcggaaggaa cggtctacac cggttttacc ttctttcgca aagtgtctca ggcgctggct
    37261 ggtttcttcc ccggctggat gctgacgcaa attggctatg tgccgaacgt cgcacaggct
    37321 gaccacacca ttgaagggtt acgccagttg atcttcatct acccaagcgc actggcggta
    37381 gtcaccattg tggcgatggg ttgcttctac agcctgaacg agaagatgta tgtccgcatt
    37441 gtggaagaga tagaagcccg taaacgcacg gcgtaataat aataaacgac gccctgcggg
    37501 gcgttataag gagtgattat gtctgaccat aatccactga cattaaaact gaatctgcgg
    37561 gaaaaaatcg cctatggtat gggcgacgtc ggttcgaatt taatgctctg catcggtact
    37621 ctgtatctcc tcaaatttta taccgatgaa ttagggatgc ctgcttacta tggcgggatt
    37681 atctttctgg tggcgaagtt ttttaccgcg tttaccgata tgctcaccgg atttttactc
    37741 gactcgcgga aaaatattgg gcctaaaggc aaatttcgtc cctttatttt atatgcagcg
    37801 gttccggcgg cgttaattgc aacgctccag tttatcgcca ccaccttttg tttgccggtt
    37861 aaaacgacga ttgccaccgc gctgtttatg atgtttggac tttcatacag tctgatgaac
    37921 tgctcgtatg gtgcgatgat cccggcaatt accaaaaacc cgaatgagcg cgcgcagctc
    37981 gcggcttacc gccagggtgg tgcaaccata gggctattga tttgtaccgt ggcgtttatt
    38041 ccgttgcagt cgcttttttc tgactcaacc gtcggttatg cctgtgcggc acttatgttc
    38101 tccattggcg gctttatttt tatgatgctg tgctacagag gcgtcaaaga gcattatgtg
    38161 gacacagcgc caaccggaca taaagccagt attctcaaat ctttttgcgc gatatttcgt
    38221 aatccaccat tgctggtttt atgcattgct aacctgtgta ctctggcggc atttaatatc
    38281 aaactggcga ttcaggtcta ttacacccag tatgtgctga atgatattaa tttattgtcg
    38341 tggatgggat tcttcagtat gggatgcatc ctcgtcggcg tattactggt gccagtaact
    38401 gtaaaatgtt ttggtaaaaa acaggtttat ttagctggca tggtgctatg ggcggtgggt
    38461 gatatactga attatttctg gggaagtaac tctttcactt tcgtcatgtt ctcttgtgtc
    38521 gccttttttg gcacggcctt tgtaaacagc ctgaactggg cactggtacc agataccgta
    38581 gattacggtg aatggaaaac cggtattcgt gccgaaggtt ctgtttatac cggttatacc
    38641 ttctttcgta aaatttctgc cgcacttgct ggcttcttgc caggcattat gctgacgcaa
    38701 attggttatg ttcccaacat cgcgcaaagc gatgcgacat tacagggttt gcgtcagctc
    38761 atttttatct ggccttgtgc actggcaatt attgctgcat tgacgatggg attcttttac
    38821 acactcaatg aaaaacggtt tgcattgatt attgaggaaa tcaaccaacg caaaaataaa
    38881 gagatggcga cagaagaaaa aacggcttcc gtaacgttat aaatgtcaat agccgctatt
    38941 tccattctgg tgggtggcgg cctccctacg tttaaaaaat ggacttatta tgaaaaagat
    39001 taatgcaata attttattat cttctctgac ctctgcctcg gtatttgctg gcgcatacgt
    39061 cgaaaacagg gaggcatata atcttgcctc cgatcagggc gaggttatgc tacgtgtggg
    39121 ttataacttc gatatgggcg cgggtattat gttaaccaat acctacacct ttcagcgaga
    39181 agatgaacta aaacatggct ataatgaaat tgaaggttgg tatcctttat tcaaaccaac
    39241 cgataaatta accatccagc ccggtggctt aattaatgat aagagtatcg gttcgggtgg
    39301 tgcagtgtat ctggacgtca actataaatt tgtaccatgg tttaatctga cagtacgaaa
    39361 tcgctataac cataataact atagttcaac agatttgagc ggggaactgg ataataatga
    39421 cacctatgaa attggcacct actggaattt taaaatcacc gataaatttt cctatacatt
    39481 tgagccacat tatttcatac gcgtaaatga ctttaatagc agcaatggaa aagatcatca
    39541 ctgggaaatc actaatacct tccgttatcg cattaatgaa cactggctgc cttatttcga
    39601 attacgttgg ttagatcgta atgtcgaacc gtaccaccgc gagcaaaacc agattcgtat
    39661 cgggacgaag tatttcttct gatgtgaaac tgccggatgc ggcgtaaacg ccttatccgg
    39721 cctacggttg atacggtttt cgttggccgg attaagcaca tccggccatt aatcaagctt
    39781 cagccgccag catctctttc ttacgttggc taattcgctt aaccagcatt acggaaacca
    39841 ccgcaccaca gaatgcgaag caggccataa tgccaaacac gattttgtag ccaataatcc
    39901 ccggatttaa atccagaatg tagccataca gactgaagca gaacatcgcc ggagcgtaac
    39961 caataaagct acccaacgcc atcgccgcgc ctgttttatt ttcagcaatt tttgcttcgc
    40021 cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag cccagcgtac
    40081 atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac agtacgagcg
    40141 cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc aggatcttat
    40201 ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg ttaatgatgc
    40261 cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat ggaatgaaga
    40321 atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt aaaagcaata agccagatag
    40381 ttttgtcttt cagcaccgag gtcatactgg tgtttttcga tgctccgtct tcttttttca
    40441 cctcgacgga cggtgcctct tctttgtcat tcagaacaaa gaaaatgata atgcctacgg
    40501 caatcacaat aagggaatag aaccagatgc ctgctttaaa acccaataag ccactgccaa
    40561 accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg ccacgccctg
    40621 tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga ctcaccgact
    40681 tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc cagacaaaga
    40741 ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct gtcgcaatga
    40801 gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata gacagaaaaa
    40861 aacccactgt cgtgacaaat gagtttaccg acatagcatt accaatttga ccattggtca
    40921 aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt aatttataaa
    40981 ttgtaccgcc acacaatgtt aatagactaa ataacgccca tttctttttc gtgagcataa
    41041 catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc gaccattcat
    41101 atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca gaagatggtt
    41161 tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat taattgcatc
    41221 ttctttttct ttacgtaacg gcgcatccag taaatgattt tctggcaatg gtgtttcact
    41281 catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga acgccggcgc
    41341 atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat catcacccag
    41401 gcatataagg tgcgttaata acgctttgaa cggcatatca ccctgacctg aaatacaggc
    41461 tttatgaccc aggccacccg gttctttaac gatcaaggca tctttgatat ggacctgggt
    41521 aatatgcggc gccatcgttt ttaaagcgtc gatgggatgc tcatttgcat tgatcatgtt
    41581 cgcaaaatca aacagtaagg aaagagattc catctcactt tctttgacca gcgacaccag
    41641 ctcatggctt tttaaatctt catgctgttc gatcgtaaaa gtcaggccgc tgtcctgata
    41701 cgtttcccgt acataggcaa tgtcgttagc gatgatcgat aatacgtcgc gcaaattacc
    41761 ttcataacgt ggataaaaac gtacggacga tgccccagtt ttcaacgcaa tggcgacggc
    41821 ttcgtcaata gatgccttat ctgaggcgct ggtttcaata tgaatatcaa ggttcagacg
    41881 gcgggcttta tctccaaagg cagagagttc tttatcgtcc atattaccaa gagaaaaacg
    41941 ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcacttg cgatatcaag
    42001 taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat aagcatgtaa
    42061 ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta ttgtcaccat
    42121 aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat tcaatatcta
    42181 tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc gctggcatac
    42241 ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt ttgaaaatca
    42301 atttttttac taccgtctct ttttggtagc acttttccca gactgaagaa aatgagtggt
    42361 tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt tcgtgataga
    42421 aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca gttgatggcg
    42481 tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggctt ttctcggtaa
    42541 acccccagga aggttcccgc ccttgctcaa ttgcggcccg ctgaaagctc gccgacagct
    42601 ccgggctgta attaaatcgc ggctgagtca cgaaccaccc tttacgatcc ttacggaaga
    42661 ttttggattc tgcttcgagg ttcagcaacg cctggcgcag cgtcatacgt ttaatgccca
    42721 gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg atgccatctt
    42781 tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc tccgccattg
    42841 tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa aaagaaagca
    42901 attaacggcg tatgactttg cagataattg tgacaaagct ctctattgac gtaatcccag
    42961 acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa aaacctgcca
    43021 gcgatggcag gttttttata actaaaaaac gaaattaatc gtctttcggt gcgcggttgg
    43081 cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag gtcggagtca
    43141 cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc atgcggatag
    43201 gcggaaccag aacaacggct tcgtcagtac cggaagcacg catgttggtc agtttcttac
    43261 cggtcaggca gtttaccgtc aggtcgttag agcggctatg aataccgata atctgacctt
    43321 cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga ccgaacagcg
    43381 cgaacgcgac cgctttaccc tgaccgttag agatcagtac gccgttctga cgctgaccca
    43441 cttcacccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga ccagtaccgg
    43501 aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg atcacgtagt
    43561 cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct ttacgttcgc
    43621 ccagcgcctg cattacagaa ccctgatgct gttcttcaac gtccagagtc acgttttcat
    43681 acggctcttg tttacgaccg tcgatttcac ggaagataac tttcggacgg gataccgcca
    43741 gttcgaaacc ttcacgacgc atgttttcga tcagaacaga caggtgcagt tcgccacgac
    43801 cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca acgttgtgta
    43861 ccagttcttt gttcagacga tccaggatct gacgagacgt tacgaactta ccttctttac
    43921 cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca tcaacggaga
    43981 gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg ttcagttcgc
    44041 caaggcccgt gatcgcaacg atatcgccag cttccgccag atcggtttcg atacgttcca
    44101 gaccgaggtg gcccagcact ttaccgactt tcgcgttgcg ggttttgcct tcgctatcga
    44161 tgatagtgac ctgctggttc ggcttcactt taccgcgctt gatgcggcca atgccgataa
    44221 cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga ccgtcaaggt
    44281 caacgtccgg cgcaggaacg tggtcaacaa tcgcctggta cagcggggtc atgtcttccg
    44341 ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa acgatcggga
    44401 agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc tgatccacaa
    44461 cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc ggcttcaggc
    44521 cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg tcaaatgcgt
    44581 caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca ccaccgaagt
    44641 cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg atagcggtgt
    44701 ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc atcacgcgct
    44761 cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg tctaccaggg
    44821 tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt tcgatcacaa
    44881 ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc actacaaaac
    44941 aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat cgctttcacg
    45001 gagcataaaa agggttatcc aaaggtcatt gcaccaacat ggtgcttaat gtttccattg
    45061 aagcactata ttggtgcaac attcacatcg tggtgcagcc cttttgcacg atggtgcgca
    45121 tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta tcttttttac
    45181 ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgaccatgac caatccagga
    45241 gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg aagtgaagtt
    45301 tgttgatttg cgcttcaccg atactaaagg taaagaacag cacgtcacta tccctgctca
    45361 tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct cgattggcgg
    45421 ctggaaaggc attaacgagt ccgacatggt gctgatgcca gacgcatcca ccgcagtgat
    45481 tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg aacctggcac
    45541 cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag attacctgcg
    45601 ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct tcctgttcga
    45661 tgacatccgt ttcggatcat ctatctctgg ttcccacgtt gctatcgacg atatcgaagg
    45721 cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc cggcagtgaa
    45781 aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt ctgaaatgtg
    45841 tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag tagcgactgc
    45901 tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg acgaaattca
    45961 gatctacaaa tatgttgtgc acaacgtagc gcaccgcttc ggtaaaaccg cgacctttat
    46021 gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt ctctgtcgaa
    46081 aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc aggcgctgta
    46141 ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa acccgaccac
    46201 caactcttat aagcgtctgg tcccgggcta tgaagcaccg gtaatgctgg cttactctgc
    46261 gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag cacgtcgtat
    46321 cgaagtacgt ttcccggatc cggcagctaa cccgtacctg tgctttgctg ccctgctgat
    46381 ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg acaaaaacct
    46441 gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct ctctggaaga
    46501 agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg tgttcactga
    46561 cgaagcaatt gatgcgtaca tcgctctgcg tcgcgaagaa aatgaccgcg tgcgcatgac
    46621 tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt tgtactaccc
    46681 ggcgcaacaa cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct
    46741 tttttctccg tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac
    46801 taaaatggtg caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct
    46861 gggcagatcc tcaactcgct gattaacagt attttgttaa tcgatgacaa cctggcgatc
    46921 cattacgcca accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt
    46981 acaccgttac cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt
    47041 ctggaggcgg ggcaaggttt taccgataac gaagtgacgc tggtcatcga cgggcgctcg
    47101 catatccttt ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggct
    47161 ccgatggata accagcgccg cttaagtcag gaacagctac agcacgccca gcaggttgct
    47221 gcccgtgatt tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt
    47281 ggcgcggcgc agctactcag caaagcgtta cctgacccat cactactcga atataccaaa
    47341 gtgattatcg aacaggcgga ccggctgcga aatctggtcg accgtctgtt ggggccgcag
    47401 ctgcccggta cgcgcgttac cgaaagtatt cacaaagtag ctgaacgcgt ggtaacgctg
    47461 gtgtcgatgg aactgccgga caacgtgcgg ttgattcgtg attacgatcc cagcctgccg
    47521 gaactggcgc acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg
    47581 ctacaggcgc tggggccgga gggtggtgaa atcattctgc gtacccgcac cgcgtttcaa
    47641 ctgaccttac acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg
    47701 ccgggcattc cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa
    47761 ggtggcaccg ggcttggctt atccatcgct cgtaatttga ttgatcagca ttcaggcaaa
    47821 attgaattta ccagttggcc agggcatacc gagttctcgg tttacctgcc tatcaggaaa
    47881 taaaggtgac gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc
    47941 gttgggtgct tgaacgtgcg ctcgctgggg caggtttaac ctgtacgacg tttgagaacg
    48001 gcgcagaagt gctggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc
    48061 gtatgccggg aatggacggg ctggcgctgc tcaagcagat taaacagcgc catccaatgc
    48121 ttccggtcat cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac
    48181 aaggggcgtt tgattatctg cccaaaccgt ttgatatcga cgaagcagtg gcgctggttg
    48241 agcgcgctat cagtcattac caggaacagc agcagccgcg taatgttcag cttaacggcc
    48301 caacgaccga tatcatcggc gaagcgccag ccatgcagga cgtgttccgt attatcggtc
    48361 ggctttcgcg ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac
    48421 tggtcgctca tgccctgcat cgccacagtc cgcgcgccaa agcgccgttt atcgcgctga
    48481 atatggcagc tatcccaaaa gatttgatcg aatcagaact gtttggccac gagaaaggcg
    48541 cgtttactgg cgcgaatacc attcgtcagg ggcgttttga acaggccgat ggcggtacat
    48601 tattcctcga cgaaattggt gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc
    48661 tggcagacgg tcagttttac cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga
    48721 ttatcgctgc cactcaccag aatctcgaac agcgagtgca ggaaggtaag ttccgtgagg
    48781 atctgttcca ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg
    48841 aagatattcc ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag
    48901 aagcgaagtt actgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca
    48961 acgtgcgcca gctggaaaac acctgccgct ggctaacggt gatggccgcc gggcaggaag
    49021 tgttgattca ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc
    49081 aaatgcaacc ggacagctgg gcgacgcttc ttgcgcagtg ggcagacaga gcgctgcgtt
    49141 ccggtcatca aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga
    49201 ccgcgttgcg acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc
    49261 gcaacaccct gacgcgtaag ttaaaagagc tggggatgga gtgattcaca gcttgtgtgt
    49321 aaagattgat tattgagcgc aaattgctgg tattttacgc tttactgttc cgataagttc
    49381 agtatgatct tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg
    49441 tatcgagtgg cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg
    49501 ccgcaatgat tgggctgttt agttgagttt cagattgctg atgaagtgct ctttgttcat
    49561 gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc aatattttca acaggttttg
    49621 taggcctgat aagcgtagcg catcaggcaa tcttgttctc agcacattga tggcgctttc
    49681 gttttctact ttgtaaacga agcgccattc actatttaaa tcacccgtga gaactgctgc
    49741 atccgcgctt tctggcgcag ataggtatca aagcacatgc aaatgttgcg gatcagcaag
    49801 cgacctttcg ccgtcacctg tattcccttc tcatccacat ccaccagccc atcttttgct
    49861 aacggggcga gcagcttgag atcttccgca aagtaatcag cgaagtgcaa atcccactgt
    49921 ttctcaatag gggcgtaatc cagacggaag ttgcagatga gcgacttaat cacatcgcgg
    49981 cgaatacagt catcacgcgt tagcgcaata ccacgccaca gcgcattgcc ttgttcatcc
    50041 acttgctgat agtactgctt caactctttc tggttctgcg cgtagcagtc gccaatcatg
    50101 ctgatggcgg aaacgcccat ccccagcaga tcggtatcgc cctgagtggt gtagccctgg
    50161 aagttacgat gcagcacgcc ttcacgctgg gccaccgcca gctcgtcatc cggacgggca
    50221 aagtgatcca taccgataaa ctgatagccc gattgcgtca ggaaggcgat ggtttcctgc
    50281 aggatatcga gtttttgctg cggactcggc aggtcagcat ctttgatttt gcgctgagca
    50341 gcaaaaatgg tcggcagatg cgcgtagtta aagacactca gacgatcggg gttcagctcc
    50401 gccacacgtt tcagggtaaa ggcgaaactc tccggcgtct gtttcggcag gccgtaaatc
    50461 aggtcgatgt tggtggaggt aaaaccaatc tcacgcgcat ggttaagcag tgcaaagatg
    50521 aactcttcat cctgctcgcg gttaaccaga cgttgcactt ctttgttgaa gtcctgcacg
    50581 cccatgctca ggcgattaaa gccttcggcg cgtaaatgat cgagtacatc cagttcgatt
    50641 tcccgcggat cgacttcgat cgaaatctcc gcatcggcat tgaactggaa gttttcgcgc
    50701 agcagcttca tcaggcggct gatttgcgct ttattcagat acgtcggcgt tccgccgccc
    50761 cagtgcaatt ggctgacgtg acgcccggcg aacagcggtg cacgatggac gatttcttgc
    50821 tccagcgcgt ccagatactg atcggccttg tgctgctggc gagtaacaat cttattgcaa
    50881 ccgcagaagt aacaaagctt atggcagaac gggatatgta cgtagagaga taatggacgc
    50941 tcaggatagc gcgccacggc ttgtaaaaac gcctgttcgc cgaagtcttc tgaaaactcc
    51001 agcgcggtcg ggtacgaggt gtatcgtggc ccggaatagt tatatttctg gatcagggcc
    51061 agatcccagt cgatttgctg tacagacatg ctcactcctt ccgatggcgt ctctggcggc
    51121 tacggcgtac cggcggatgc ccgtttcggg tcatcagccg gttgcgcagc cacttttgtc
    51181 gccgcgacaa ccgctgtagt ttaacgaata accacaccag ataacatata accggaaggg
    51241 ttatcagcag aacgggaagg cccacggcgg caatccgtta gttgccccgc agcagacgca
    51301 tcatgtcttc ttgcttctcg tcttcttcct cttcttcgtc atcgtcataa gagaggccga
    51361 gtttctgcat caactcatca atacgatcca gtttagcatc cacccaggat tgctcttcgg
    51421 cgctcagggt ttcgcctgct tccagacgtt ccagcagcgc atccagacgc tcatccgttt
    51481 ccagtaattc caactccgcc tgcggtgaaa gcataggttt ctcactcttc ggtttgtgct
    51541 gtttggtgac tttttcagtc acgcccaatg gaataggggt tttactgcca atacgtggat
    51601 cttttggtgc gttctggcct ttgctgcctg acgtggtgtt tccgcccgct gcgcggctgc
    51661 ccggcgcatg accacgacgt tttttctgac gcttgcgatc acgagcttcc tgatccagct
    51721 cctcgcgtgt ttttcgacgc gcttttgcat gacctttgct gcgtgagttt gaagatgatg
    51781 gtttcataat ggttcgtctt ttagccgttt tattcagtat agatttgcgg cggaatctaa
    51841 cagaaagcaa gcaaagaaaa aaggcgacag attactctgt cgcctttttt cctgactcat
    51901 aacccttaac gggtcttaca atccttgcag gccgccaaca caccctgttt ttcctttagc
    51961 cggaacaatc cgtgttgatt ccatttccgt ttaattacgt ctccggacgt ttgtcttcct
    52021 gacaatcctc tgtcttcgcc tcctggcgct cctgaccctc atcctgagtc attgttcctg
    52081 ttagcgtcct cgctctatgg gtgctacttt acgcctttgc tttaaacaaa caatcaaccg
    52141 ccatcagtat ttgcacattt tcagagtttt atataaaagc aattcactga tttatatagc
    52201 ttatttgaca tgcatcgcag aattttctct gctatttcgc ataaagacta tcggcaatgt
    52261 ctcacaaagc gtatggcttt tacttacaac gctcacgccg aaaaatccgc cttttattat
    52321 ggttcaggta taatcgccac aaagctttga ctgacggaga cgaccgcttt gactaatttg
    52381 aattatcaac agacgcattt tgtgatgagt gcgcctgata ttcgccacct accttccgat
    52441 accggaattg aagtggcttt tgcaggccgt tccaacgcag gtaaatccag cgcgctgaac
    52501 acgctgacta accagaaaag cctggctcgt acctcaaaaa ccccagggcg cacccagctt
    52561 atcaacctgt ttgaagtggc tgacggcaag cgtctggttg acttgcctgg gtacggttat
    52621 gcggaagtcc cggaagagat gaagcgcaaa tggcagcgtg cgctcggcga atacctcgaa
    52681 aaacgtcaga gcctgcaagg tctggtggtg ctaatggata ttcgccatcc gctgaaagat
    52741 ttggatcagc agatgattga gtgggcggta gacagcaata tcgccgttct ggtgctgctg
    52801 accaaagcgg acaaactggc aagcggcgca cgtaaagcgc aattgaatat ggtgcgtgaa
    52861 gctgtactgg cgtttaacgg tgatgtgcag gttgaaacgt tttcttcgtt gaagaaacaa
    52921 ggcgtggaca agctgcggca gaaactggat acctggttta gcgagatgca gcctgtagaa
    52981 gaaacgcagg acggcgaata attttcttgc cttaatgctt gtgccggatg tggcgtatcc
    53041 ggcccgtaaa ttcaatagat tacaattcta ccgttggcct gataaacata gcgcatcagg
    53101 cattacggat cttttctttc gcccaataaa aaacgcccca gtcattactg actggggcgg
    53161 ctaaaatatt cagccaaatc cgattacgtg aagtaaaagg tctgaaagat agaacatctt
    53221 acctctgtac cctacgcgac taactttact cttttttgtt cagttcagaa agcacttttt
    53281 gtaatttttt ttcagttgtt gcataggaat ttctaatagc catcacaaaa cgtgacagct
    53341 tatgttgctt acttacgaaa aaagggcatg ttcaggcgaa tcttagtgcg cctgatccca
    53401 gttttcgcca ctccccactt ccaccagcaa cggcacatcc agacgggtac agttttccat
    53461 cagttgatga atctgcttcg cgacggcatc aacatcatct ttatgaactt caaataccag
    53521 ttcatcgtgt acctgcatga tcatacgtac acgcggttgc tcagcctgta accacgcatc
    53581 aacggcaatc atcgcccgtt tgataatgtc ggcggcggtt ccctgcattg gcgcgttaat
    53641 ggctgcacgt tcagccgctg cacgacgagc accattgctg gatttgatat ccggcagata
    53701 cagacggcgt ccgtccagcg tttcaacgta gccctgctct ttcgcctgag cacgggtgcg
    53761 ttccatatac tccagcacgc cagggtagcg ttcgaagtaa aggtccatgt acttctgcgc
    53821 ttctttacgt ggaatgttca attgccgcgc cagaccgaaa gcactcatgc cataaatcag
    53881 accaaagttg atcgctttcg cgctacggcg ttgctcgctg gtgacggttt ccagtggcaa
    53941 accaaacact tctgccgccg ttgcccggtg gatatctttt ccttccgcga atgcggtcag
    54001 caagcctttg tcacgcgaaa gatgcgccat aatgcgcagt tcaatctgcg agtagtccgc
    54061 tgagacaatc acataatcct ctggcgcaat aaacgcctgg cggatacgac gaccttcttc
    54121 gttacgcacc ggaatgtttt gcaggttagg atcggttgac gataaacgtc ccgttgcagt
    54181 tactgcctgg tgataagagg tatgcacacg cccggttttc gggttgatca tcagcggcag
    54241 cttgtcggtg taggtcgatt tcagcttcgc cagaccacga tactccagaa tcacttttgg
    54301 caacggatag tccagcgcca gttcttccag tacctcttcc gacgttgacg gcgcgccacc
    54361 cggcgttttc ttcagcggtt taataccctg tttttcaaac agaatggttt gcaactgctt
    54421 ggtggaagaa aggttaaatt cttcacctgc aatttcatgc gctttctttt ccagctcagc
    54481 cagacgcaag gtgagctctt cagaatgatt gtgcagcact ttcggatcga tcttcacacc
    54541 gttacgttca atgcgtgaaa gcactggcac cagcggcatt tcgatattct cgaagacgtt
    54601 caacggccct ttgtgttttt gcagatccgg ccacattttc agatgcaact gcaaggtgac
    54661 atcagcatct tcggcggcgt aacgtccggc ttcttcgagg gcaatctggt taaaggtcag
    54721 ttgatttttg cctttacccg caatctcttc aaaagtgatg gttttgtgct tcaaccaacg
    54781 ttccgcgagg ctgtccatat cgtgacgccc ggcaacgcta ttgagaatgt aggactccag
    54841 catggtatca aacgcaatcc cacgcagctc aatgccgtaa ttcgccagaa taccgcgatc
    54901 gtatttcagg ttttgcccga ccttcagcgc cttttcatct tccagcagcg gttttagcaa
    54961 ctcgagtgca cgctcgcgag agatttgatc gggcgcatca agataatcat gagcaaccgg
    55021 aatatatgcc gctacgcctg gctcgatagc aaaagaaagc ccgaccaggt tagcagagat
    55081 gttatcaagg ctgtcggttt cggtatcaaa tgcaaatacc ggcgcttttt ccagcttcgc
    55141 aatccacgct ttcagtgttt cttcatcaag gatggtgacg tagttgtcat aagaaatcac
    55201 cgttgccgtc acttctggcg cttcgtctgc aacactggtt tcctgcggct tcgcggctgg
    55261 ttttgcccct ttggcctgta accatttgcc cgcttcgaca tcagcagtcc agcgtttgaa
    55321 ctcatacttt ttgaacagcc ccaacaactc ttccgctgcc ggttgctgca cttccagttg
    55381 ttcacaggtc agctccagtt caacgtcggt tttaatcgtc gccagctggt atgagagata
    55441 agcaacttct ttgttttgct cgagcttcgc tgccattgtt ttcgcgccac ggaagctcaa
    55501 cccagcaatt ttttctggct cggcatacag cgtatccagc ccgcccagac cttgcagcaa
    55561 tgcctgcgcg gttttttcac cgacgcccgg tacgccagga atgttatcag aggagtcacc
    55621 catcagcgcc aggaaatcga tgatcagttc tggcggcacg ccgtacttat tcaccacctc
    55681 ttccggtccg aggatggtat tcgtcatggt attgataagc gtaatatttg gcgtcaccag
    55741 ctgcgccata tctttatcgc cagtgctgat cagcaccgga cgcccggctt tttcggcttc
    55801 gcgcgccaga gtaccgataa cgtcgtccgc ttctacgcca gaaaccgcca gcagcggcag
    55861 tcccatcgct ttaaccatcg cgtgcagagg ttcgatttgt gctctcagat cgtccggcat
    55921 tggcgggcga tgtgatttgt aatgttcaaa cagttcatca cgaaaggttt ttcccttggc
    55981 gtcaaagacc accgctgcat gcgtcggttt atattgcatg atcagactgc gcagcatgtt
    56041 gaggacacca tacatcgcac cggtcggctc gcctgcgctg ttagtcagcg gaggaaacgc
    56101 gtgatatgcg cgataaagat aagatgaacc atctacaagg ataagtggat tttgggggat
    56161 ctgaaccata atgtccgtgc ctgtttatca gattatgggt aaaggatgcc acagacagga
    56221 tgaaaacatg agtttttcac cgcttttcgt gaaaatcttt tgtagatctt ctggatcgct
    56281 cgcaaatcgt catgtggata actttgtgca taaattattt acatcgcttt tgatttatta
    56341 tcatgctttt tacagcatga tgtttgtctt tgtttttcat gcagttaaaa ataaagccat
    56401 ccctaataat tgagataatg ggattttttc taatgtggat agatatgaat tatttttctc
    56461 cttaaggatc atccgttatt tgggtcgttt tctgctaaaa tctgcgcctt gcgcttcttt
    56521 agcgcactgt ctatggctaa ccttttgaat aaattcatta tgacgagaat actcgctgcg
    56581 ataacccttt tgctgagtat cgtattgacc attttggtca ctattttttg ttccgtcccg
    56641 atcatcattg ccgggattgt aaaactattg ctacctgtgc cagtcatttg gcgaaaggtt
    56701 tcgcgttttt gtgattttat gatgtattgc tggtgtgaag gtctggcggt attactgcat
    56761 ctgaacccac acctgcaatg ggaagttcac gggctggaag ggttaagtaa gaagaactgg
    56821 tatctgctta tttgtaacca ccgtagttgg gcagatattg tcgtactgtg cgtgctgttt
    56881 cgtaagcaca ttccgatgaa taaatatttc ctcaagcaac agctggcctg ggtgccattt
    56941 cttggcctgg cgtgctgggc gctggatatg ccgtttatga agcgttattc ccgcgcttat
    57001 ttattacgtc atccggagcg acgtggtaaa gatgttgaaa ccactcggcg ttcttgtgaa
    57061 aagtttcgcc tgcatcccac caccattgtc aatttcgttg aaggctcccg tttcacgcaa
    57121 gaaaaacatc agcaaaccca ctccactttt caaaacttgt tgccaccaaa ggcggcaggc
    57181 attgcgatgg cgctaaatgt actgggtaaa caattcgata aactgcttaa tgttacgctg
    57241 tgttatccgg acaataaccg tcagccgttc ttcgatatgt taagcggtaa actgacgcga
    57301 attgtcgttc atgtggattt acagcctatt gccgatgagt tacatggcga ctacatcaat
    57361 gacaaaagct ttaagcgcca ttttcagcaa tggctgaact cactgtggca ggaaaaagac
    57421 cgacttctga cgtcgttgat gtcatcacag cggcaggaca agtaaatttt gctcgcctct
    57481 gaatgagcag aggcgagtga gtattttaat gaacaaaacg tccggcacga gacataaatt
    57541 cttcttcgct catctcctgt ccgttaaaaa caactttacc cggtgtataa cgcagttgca
    57601 gcgaggcggt attgttgtca atcgtgatta actgaaacat ctgacctaag gtcatcatcc
    57661 cgctgatttg tttatcagcc cgcttttgcg ctttttccgc atccattcct tcagataaat
    57721 taagctgttt tgcggtttct gtggcgacat taagcggtaa cttcacatca aaattgagcg
    57781 atttaatatc tttgtttgtg gatgatgaag atttggctgg gtcggcaata ctgatatcca
    57841 gattggcatt tagttcgccg agtgcgttct tccattttac cggttgttta atggtcggct
    57901 cacttttttg taataacggt aaatactctt tgaacaatgc agcattcact tcttgcagag
    57961 caacttcatc gtttgctaac tcagggtgtg cagcaagctg cttctgcatt gcaatgttat
    58021 actggatgat aaattggcgc accgctgagg gatcaataga ttcagcaatc aatgaccact
    58081 caccactacc gagttgctga ttcccttttg ttaacttatc aagttcgtag gtcagattga
    58141 cattgatata atctttgtct ttaacgcgat ccagcatggt ttttgcaacg aaagcatcga
    58201 tttgggcaac gtcttccccg tgattgataa tgttaatctg attaaatttc gactcgcttt
    58261 caccaaccgg aaatttcttt tcttctaatc gcgccagaga atcaatggta aaactttttg
    58321 ccgttacttt gttcattggt gataattgca gaacaatatc ttcgactttg ccttcaatat
    58381 ccaggttaga caatgaacta ccatcaccat taaaagtaaa ctggccctca ccaaaagaaa
    58441 actttccata ctctgcaggg gcaacatcta atgtcgtaga gaatgagcca ccaaacgcga
    58501 atcgcaatgt cgcttccacg ggcgatttat ttttcgcggc gataaatagc ggttgtgtca
    58561 gttcattatt cactaagttc acttttgccg ctgccagtgc tgggatgaca ttaccatgca
    58621 ttaacattgt gatgggcaac ggaccgtgct caacatccac atcaaataca actttctggc
    58681 ctggcttgat attgagatcg ggtgcaccgt tatcgaatgt catttgcatt tgaaaacggg
    58741 aattaaaaaa gcctttgtca aaattttgat aacttaacgt catatcgtag gcatgttcac
    58801 ctttcttttt agcatcgtta aaatctttaa taaatttttc gacaccaggc tgaatttgtg
    58861 tacctgtgta ccatgtgcca ccaccccaga ttacggctaa cgcaacaata acacctgtag
    58921 ctgacttacg tatcatataa atattccctg ataaaacagc aaaactttaa atatccgctt
    58981 atgctgaaat aatgtccagc ggcaggacgc attatcagaa tgtatttata tttattaaat
    59041 aataaaaaaa acccgtgaat attcacgggc cttatgtaat ttacattgaa ctattttttc
    59101 tcggacagat atttcactgt atcagcatac tgctgaacaa aaacatccat attgctggta
    59161 tccataccct gcggattcag ctgatattta ccgttaacaa acatcgccgg aacgccacgc
    59221 aattgcacgt cagctgcagc tttttcctgc tgagcgacca gagatttgac cacgaagctg
    59281 ttccacgccg cgtcgtactc ttcaccttta atacctgcgt tgataaatac atcgcggata
    59341 tcagatgctg aacgaatggt ctgggttttc tgtacgcctt caaacagcgg aacagtgact
    59401 ttgtcttcca cgcccagcgc catcgccaca gcccatgcct gagtcagatc tttgcccagg
    59461 tcaccaccca tgaagttgac gtggtattta gtcatcttca cgccttccgg cagttttttc
    59521 ttcacattat cagaaatatg cagaacttct tcaaactgat agcagtgcgg acagaagaaa
    59581 gagaaaaact ccagcacttg cggcgcgcca gctaccggtt tttccagggt agtgtactgt
    59641 ttaccatctt catactgcgc cgccgatgcg ctaaacgcta aaactaaacc agccagcgcc
    59701 agccaaatct ttttcatgat ctactctctc cgattaatac ataggtgtta attgtaaagg
    59761 gggttcttgt agaacttttg cctgttctat aaaagtcgcc gtttgtcgca gccagtaatc
    59821 ttccccggtt aaccacggga aatttttcgg gaacgcggga tcagcccaac gccgcattag
    59881 ccaggcaaga taataaacca aacgcatggc gcgtaaaggt tcaatcagtc cgatttcagc
    59941 ggtgtcgaac tcgctaaatt cttcataagc ttcaataata gtttccagtt gcatccgctg
    60001 ctcggcttta tcgccattga gcaacatcca caaatcctga atagctggac cattacgtgc
    60061 atcatccaga tcaacaaaca ttggaccatc gcgccagaga atattcccgg cgtggcagtc
    60121 tccatgtagc cgcagaacgg tgaagtcttc ccgccagtgt gctgtaacgg cggcgatcag
    60181 ctcatctgtc gctttcagga atgccgcttt caacccggaa ggcatcagtg tagcgtcctc
    60241 aaacagcttg cgtggctcaa tgagatattc attcagaccg atggtcgggc gatggataaa
    60301 aagctgtttg cgccccgtct ggtgcatacg ccctaaataa cgcccaaccg cttccatctg
    60361 atcgatatta tcagcttcga actggcgacc accgacgctt ggaaaaacag cgaaataaaa
    60421 tccctgatga ttcaataaag tctgaccgtt aaaggccaca ggtgctgcga ccggaacttc
    60481 atcatttacc aactgcaacg caaattgatg ttcttcgagg atttgatcgg ctgtccaacg
    60541 ttcagggcga taaaatttga cgacaaaacg tcgacgatct tcgtcctgaa attgatagac
    60601 acggttttca tagctgttaa gcggggtaag accggaatcc acccggatcc cttgctcaaa
    60661 tagagcatcc atgatggtat ccgggtgtag tgtctggaaa gtaaaagcgc tgttgttcat
    60721 cccatcatcc ggaaaacatt acgaatgatg aaggatatca tttcacgatg attttggtgg
    60781 caccgcttac aagcttttac tctttaatta cgccacgagc gcgtaacagc gccgttttga
    60841 agtcttcttc ataatctttc tgcaaacccg ggatcaccgc atctttcgcg gaatcacgca
    60901 ttttcaggtg atagatcaga atgtcatccg tcaaatccgc cagttcgccg tcaaaacctg
    60961 actctttcgc cagtttctgc aaaaattgca acaggttaag gtctggctct ttttgccagg
    61021 ctggctggag gagttcaata acttcattca gacgtttaca tttcatagta atgctcctta
    61081 ctcttgagac agacacgtta gcagggtcaa tcccacaata aaagaggcga tatcggtgaa
    61141 tctgatgacg acgataacag gcgttgtgct ggcaggcggt aaagccagac gaatgggcgg
    61201 cgtagataaa ggattgcttg aattaaacgg caaaccatta tggcaacatg tcgctgacgc
    61261 gcttatgacg cagctctctc acgtcgtggt taatgccaat cgccatcagg aaatctatca
    61321 ggcaagcggt ctgaaagtga ttgaagattc actggcggat tacccaggcc ctctggcagg
    61381 aatgctttca gtaatgcagc aggaagcagg cgagtggttt ttgttttgtc cgtgcgatac
    61441 tccttatatt cccgaggatt tagcagcccg gcttaatcat cagcgcaaag atgcgcctgt
    61501 cgtgtgggtc cacgacggtg aacgcgatca cccgactatt gctctggtaa accgcgcaat
    61561 tgagccttta ttactggaat atctgcaagc aggagaacgc cgggtaatgg catttatgcg
    61621 tctggctggc ggtcatgcgg ttgatttcag cgataataaa gatgcattta ttaacgtgaa
    61681 tacgccagag gagcttgccc gatggcagga aaaacgatga taccgttact cgcctttgcc
    61741 gcgtggagtg gtaccggaaa aactacgctt ttgaaacaac tgatcccggc attatgcgcc
    61801 agagggatcc gtccagggct gattaagcat acgcaccatg atatggatgt tgataagcca
    61861 ggcaaagata gctatgagct gcgcaaggct ggcgcggcac aaaccatcgt tgccagccag
    61921 caacgatggg ccttgatgac ggaaacacca gacgaagaag agctggatct acagtttctc
    61981 gcaagtcgaa tggatacctc aaagctggat ttgattctgg tcgaagggtt taagcatgaa
    62041 gagatcgcaa agattgtgct gtttcgcgat ggagccggac atcgacctga agaattagtg
    62101 atagacaggc atgttattgc tgtagccagt gatgtgccgc ttaatcttga tgtcgcatta
    62161 ctggatatta atgatgttga ggggctggct gattttgtgg tggagtggat gcaaaagcag
    62221 aacggataag caagagtagg ctggataaga cgcgccagcg ttgcatccgg catttttttc
    62281 tgcgccaatg caaaaaggcc atcctgacgg atggccttct gcttaatttg atgcctggca
    62341 gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc gtttcacttc
    62401 tgagttcggc atggggtcag gtgggaccac cgcgctaagg ccgccaggca aattctgttt
    62461 tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg ctgaaaatct ttctctcatc
    62521 cgccaaaaca
//
LOCUS       DAEVZA010000010        60753 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEVZA010000010 DAEVZA010000000
VERSION     DAEVZA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.010"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..561)
                     /gene="fimE"
                     /locus_tag="LAF92_000683"
     CDS             complement(<1..561)
                     /gene="fimE"
                     /locus_tag="LAF92_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBI9789739.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNN"
     gene            complement(1038..1640)
                     /gene="fimB"
                     /locus_tag="LAF92_000684"
     CDS             complement(1038..1640)
                     /gene="fimB"
                     /locus_tag="LAF92_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HBI9789740.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            3094..3810
                     /gene="nanC"
                     /locus_tag="LAF92_000685"
     CDS             3094..3810
                     /gene="nanC"
                     /locus_tag="LAF92_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBI9789741.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            3830..4936
                     /gene="nanM"
                     /locus_tag="LAF92_000686"
     CDS             3830..4936
                     /gene="nanM"
                     /locus_tag="LAF92_000686"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBI9789742.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLAPVASPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            5001..5981
                     /locus_tag="LAF92_000687"
     CDS             5001..5981
                     /locus_tag="LAF92_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBI9789743.1"
                     /translation="MDTIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(5989..6111)
                     /locus_tag="LAF92_000688"
     CDS             complement(5989..6111)
                     /locus_tag="LAF92_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI9789744.1"
                     /translation="MISFVFLLPVYPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            complement(6658..7605)
                     /locus_tag="LAF92_000689"
     CDS             complement(6658..7605)
                     /locus_tag="LAF92_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016160008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBI9789745.1"
                     /translation="MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTH
                     QLVFAGGTALAKSSVALNRMSEDVDIKLVPKDAFQALSRTQRKNTRKEIVQAVSSAIA
                     SSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
                     DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHI
                     YDTNCIVETSHVDMSQLVDFVQQTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNP
                     LYEMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA"
     gene            complement(7598..7993)
                     /locus_tag="LAF92_000690"
     CDS             complement(7598..7993)
                     /locus_tag="LAF92_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBI9789746.1"
                     /translation="MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREG
                     QLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVETLERLKVHYRFAGATQAYNSGT
                     STQIPASPEIKTPRRFKRVLSVGNSRLNG"
     gene            complement(8063..8896)
                     /locus_tag="LAF92_000691"
     CDS             complement(8063..8896)
                     /locus_tag="LAF92_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBI9789747.1"
                     /translation="MQTEAEVLTDHNELICSTSIERIVTGRDSALNQIATLIQKLHDI
                     SSLTSSIGGDVAGTWAMRNGYAFDCWLMQPTDKAMPVITRNIDRSIWRDLMLKSGMLT
                     LMDAEARSQWAKNLEEGDLPAISEANILSTFEQLHHNKQDVFERGIINVFKGLSWDYK
                     TNNPCYFGKKIIVNNLVKHDRWGYSLNWGWRRDQLADLERMFYLLDGKTIPDNRHDVS
                     IRFMDFVRDNPHQQIFEDELFTIRYFQKGSGHITFKRLDLVEKMNDIVAKHFPSALSA
                     K"
     gene            complement(9027..9362)
                     /locus_tag="LAF92_000692"
     CDS             complement(9027..9362)
                     /locus_tag="LAF92_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBI9789748.1"
                     /translation="MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFN
                     NEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGFSWQEQTPYLRAVDILRARQATG
                     LLRRCHNTP"
     gene            complement(9393..9728)
                     /locus_tag="LAF92_000693"
     CDS             complement(9393..9728)
                     /locus_tag="LAF92_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBI9789749.1"
                     /translation="MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITG
                     KFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQRTVTLYAKGLTCEADTLGSCGYV
                     YIAIYPTQR"
     gene            complement(9728..10201)
                     /gene="radC"
                     /locus_tag="LAF92_000694"
     CDS             complement(9728..10201)
                     /gene="radC"
                     /locus_tag="LAF92_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBI9789750.1"
                     /translation="MSKLILTPVFSVSEQRVIRRAMRLLEKYQRNPGESFLSSDATKT
                     WLQLHLARQEREMFVVLYLDNQNRLLEHETLFLGTINSTEVHPREIVKSALRYNAAAV
                     ILAHNHPSGVAEPSRADRLITTNIVKTLDLVKVRVLDHFIIGAGDVLSFAERGWL"
     gene            complement(10231..11049)
                     /locus_tag="LAF92_000695"
     CDS             complement(10231..11049)
                     /locus_tag="LAF92_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBI9789751.1"
                     /translation="MKLASRFGHVNQIRRDRPLTHDEMMQYVPSVFGENKHESRSEHY
                     TYIPTITLLDNLQREGFHPFFACQTRVRDVGKREHTKHMLHLRREDQITRNQVPEIIL
                     LNSHDGSSSYQMLPGLFRSVCCNGLVCGESFGEVRVPHKGNVVEKVIEGAYEVLSVFD
                     QVEEKRDAMQSLMLPPPAQQALAKAALTYRFGEEHQPVTESQLLAPRRWQDESGDLWT
                     TYQRIQENLIKGGLNGRTAKGGRTHTRAIKGIDGDIKLNRALWVMAENMLQLAS"
     gene            complement(11285..12238)
                     /locus_tag="LAF92_000696"
     CDS             complement(11285..12238)
                     /locus_tag="LAF92_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789752.1"
                     /translation="MAVNVRSSIAVKDIRTSRFLFWYIRKNIHGANQFAFDGNNPDTS
                     LSERIINTALEAGYPDPIQRSNFIRSLELAINTTLLPEKYFSWLKENERATFWLWGVM
                     CLDYRCMESINEKLGTNLNTNWYQKAGITDSPASHSERYSIIVALLDYINIETNQAPL
                     MRQWLYERLVFWRANEHQRIKLRWLNEDNDEVCIWAYNYINKFQKEHGSNTGSQLDPV
                     QIPEPLNSIETYHAIYAMLDLWSADDDLQQKAVKKINKAFYQKNFRRKLSEKRERESI
                     SDTHKERLDFLVKFYKSDKISVIERLIDERVQGIETRLSRG"
     gene            12831..13445
                     /locus_tag="LAF92_000697"
     CDS             12831..13445
                     /locus_tag="LAF92_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBI9789753.1"
                     /translation="MKLNERYGKPNAHYMKRLEEITRYAMDDYARILAFRVDLLLPVG
                     NSHKGYDSAVITRFIASLKAQIAAYLQRRRRDGKRVYPCKLRYAWVREFGELNGNKHY
                     HMLLLVNREVFRNTMLNTGNSAQPRGLLVGMVARAWAVALRRTGDMANKVPFHLPGVS
                     YELDRRNGTNTDFFRSYIYHISYLAKEYTKNHDDGERNFGCSQG"
     gene            13563..13781
                     /locus_tag="LAF92_000698"
     CDS             13563..13781
                     /locus_tag="LAF92_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBI9789754.1"
                     /translation="MNNQPNTDTDLMNDKLVTMAFITTFTGLTDKWFYKLISEGKFPK
                     PIKLGRSSRWRESEVKRWLTERIAESRY"
     gene            complement(13984..14769)
                     /locus_tag="LAF92_000699"
     CDS             complement(13984..14769)
                     /locus_tag="LAF92_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789755.1"
                     /translation="MKPIIIPALFVSLFAFQPAYAVGAYDLQCLLDNGEQMTLSHVSN
                     TVYISFENPGGDSEESGSVIKLDIPSGEAKQTLAANPGAGTASFTLRGENEDIEGAVA
                     VNYSEYDGTGDAYYTAMNAMGQEISTVSCKPGTIKVSRSLLQNGINGVGSQQANKPAP
                     SQQQQAQQSMTPPFKVQIGSSVSNEGWNTRYGVIQLTITDDNVVLKSIRVNRGNCKME
                     SVGNRTLPAKYKFGDVATFKYMKCDRIIEADIVTDTGSWTFNS"
     gene            complement(14781..15794)
                     /locus_tag="LAF92_000700"
     CDS             complement(14781..15794)
                     /locus_tag="LAF92_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBI9789756.1"
                     /translation="MDDPVKGEGRMITDASGVTRREGGILGMWSNIFPERNGSNEQKG
                     DSKPHIPDIRDILDIFPQPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKHSLKIIDIPGVGENAQRDKEYEDLYRNLLPSLDL
                     ILWIIKGDDRAFSADEHFYNNVLLPAGGGERVLFVLNQVDKIEPFREWDTLLHQPSPA
                     QRINIEKKEAYITERFGFTHYPVTPVSADEGYNILRLVETIIRALPDRAKSSTASQFK
                     DEFKTEEVKTEAKNGFGSVISGILDDVIDSFPLPKPVKELTRKGKDILIEWAGNAWDH
                     FFG"
     gene            complement(15833..16516)
                     /locus_tag="LAF92_000701"
     CDS             complement(15833..16516)
                     /locus_tag="LAF92_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBI9789757.1"
                     /translation="MKTQNDHYDRLSVRLSIIITRLLAGESLELKALADEFGVSERTL
                     QRDFQQRLIHLDLEHSSGSYRLTRRTGREHSPNTFAFIRNSGISGIIPAQNRRLIRLI
                     TSNSGSSPCLIGHGGLPSPVIQTTSFLELAEAIRDYRAVTLLVNGKSYEAVAPRRLIF
                     QSKNWFLVATHTRNLQVFRMEDVNAVTVLDTSFRRKPELDALTASEEFISALPHFRFI
                     SDVIQTFRE"
     gene            complement(16831..17808)
                     /locus_tag="LAF92_000702"
     CDS             complement(16831..17808)
                     /locus_tag="LAF92_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBI9789758.1"
                     /translation="MISLVFSYSHVDEALRNELEKHLSPLKRMGKITTWHDRRIIPGQ
                     EFENQIDHYFSQADIILLLISSDFIASDYCYQVEMTNALERHKRGEAVVIPVILRECA
                     WKMLPFGRILAATVDGKPIEKFASHDEGYVQVVDAVSRAIANLEAKKPQQIVHAPAPA
                     NPILQATDTVFTPRSSNLSLPKRFTDLDKDRACRDGFEYVARYFEGSLTELKKRHTGL
                     EVDFNKRDANAFTCAIYIGGRKVGQCAIWLNSGRNMGIGDIGYSQDGVIGNAYNEILT
                     IDNNGQNLGFRPSMGVFSHGYSRDTLLTNEGMADHLWDMFFEPIKQRAR"
     gene            18022..19728
                     /locus_tag="LAF92_000703"
     CDS             18022..19728
                     /locus_tag="LAF92_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBI9789759.1"
                     /translation="MDNAEQQFLNDLDTKFWKAADKLRANMDAANYKHVVLGLIFLKY
                     VSDAFDARQKELKGLFEDKSNPDNIYALSRDDYDTEEAYQQAIAEELEVQDYYTEKNI
                     FWVPKLARWETLKTNAALPVGTVLGKDDSGKDIVMTSVSKLIDIALDTIENSNPKLKN
                     ILNRIGHYQLGNDLLISLINVFSDTSFSEPKYNGVKLNLKSKDILGHVYEYFLGQFAL
                     AEGKQGGQYYTPKSIVTLIVEMLQPYNGRVYDPAMGSGGFFVSSDRFIEAHADEKHYN
                     AAEQKRNISVYGQESNPTTWRLAAMNMVIRGIDFNFGKQNADTFLNDQHPDLRADFVM
                     ANPPFNMKEWWNAKLEDDVRWQYGTPPQGNANFAWMQHMIHHLAPKGSMALLLANGSM
                     SSNTNSEGEIRRAIIEADLVECMVALPGQLFTNTQIPACIWLLTKDKSGGNGKAHRKG
                     EVLFIDARQIGFMKDRVLRDFSTDDIKKIADTFHAWQTDKNYEDEAGFCFAATLDDIK
                     KNDFILTPGRYISEVNQDDDNEQFAEKMARLTAQLKDQLEEREKLEVQIKVNLAEMGY
                     EF"
     gene            19718..21052
                     /locus_tag="LAF92_000704"
     CDS             19718..21052
                     /locus_tag="LAF92_000704"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBI9789760.1"
                     /translation="MNSDWKIVKLGDVCTIRRGSSPRPIVDYMAPKGMPWVKISDATE
                     SKSRYISKTKEFIKPEGISRSVIVNKGDLILSNSGTAGLPKFMEITACIHDGWQVFTE
                     LVDIDKEFLYYVLLNIRSKLLHNAYDSTMKNLTLDMVRDAQVRLPQIKEQKRIAGVLS
                     SIDGMIECNQNVNQTLEQMAQALFKSWFIDFEPVKAKIAVLEAGGSQEDATLAAMTAI
                     SGKDADSLTIFKREYPEKYAELRATAELFPPTMQESELGDIPAGWSSQRMSNIATLHY
                     GKALKKTERIDGPYPVYGSGGITGSHNAYLVEGPGIIVGRKGSIGTIYWEDGKFHPID
                     TVYYVATKEEIPLSYLYYLMKTLNLPSMNTDAAVPGLNRDNVYRLEVLKPTQSILNRF
                     DNHIATIRNSIQKNNNTIVSLVGLRDTLLPKLLSGEIPLPEAEELAKEADYV"
     gene            21045..22238
                     /locus_tag="LAF92_000705"
     CDS             21045..22238
                     /locus_tag="LAF92_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HBI9789761.1"
                     /translation="MFKVDQPSNSLQPLQETTFTNAGFTERYHLQEWLAKHPQALTRS
                     SDDELLIIQKEFAGFDDTKERLDLLAIDKDGNLVIIENKLDDSGRDVVWQALKYAGYC
                     ANLRKEHIVEIFQRYLDQNDPEETRPASDVLAEFLEHESLDEVQINQSRSQRVMMVAA
                     NFRKEVTNTALWLMQFNIRVQCFKVTPYRYGNDVFIDIRQVIPTPEAESYMIGMAQKE
                     AEEQSTSGELKQSQRLRKAFWTQMLEKFHASDCTLYNNISPSMDHWLCAGSGISGMPY
                     SLIFSKKEIRVELWISRASADINTMALNWLEQHRTSVEEVFGNPLEWLSLPGKKACRI
                     QYRQKIDGFQQENWPQMIDWLVEHMTRFENALSPYLPQLNQLIKQTFNGHDEAENTTE
                     EAEHV"
     gene            22254..25388
                     /locus_tag="LAF92_000706"
     CDS             22254..25388
                     /locus_tag="LAF92_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBI9789762.1"
                     /translation="MLTEDDLEQLCLGWFAEQGWEIYHGPDIAPDGSNPQRASYHDVF
                     LTPVLNACLQKINPHLPTDVFEQVVSRITRAESPDLIASNKAFHHWLLEGVPVQYRRD
                     EELIHDHALLVDFNQQQNNRFMVVNQMTISGTKQPRRPDIICFINGLPLAVVELKSPI
                     DDKVDIWDAYHQLQTYKDEIRDLFISNEALIVSDGYLARVGSLTATEERFMPWKTISN
                     ENDKPLLEWQLETMVRGFFNRELLLDYIRYFVLFENNDERLIKKIAGYHQFHAVREAV
                     QATIVASTGKQLVLHSNIQPGSKKAGVVWHTQGSGKSISMCCYVGKLLQQPEMGNPTI
                     VVVTDRNDLDGQLYGTFCQAQELLKQMPVQADDRETLRDLLATRDAGGIIFTTVQKFS
                     PEEGENAHPILNGRSNIVVISDEAHRSQYGMKANLDRKTGVYKYGYAKHMRDALPNAS
                     FLGFTGTPVSAEDRDTRAVFGDYVSIYDIQDAVDDGATVPIHYESRLAKLDLNHEELA
                     ALSDQVEELIEDDELDQREKTKGEWSRLEKLVGAKPRLQQIAADLISHFEKRNTVMDG
                     KAMIVAMSREICVHLYDEIIALRPEWHDDDPEKGQIKIVMTGTASDKAMLQPHIYNKR
                     TKKRLESRFKNVNDPLKLVIVRDMWLTGFDAPCCHTMYIDKPMRGHNLMQAIARVNRV
                     FKDKPGGLVVDYIGIANELKLALKTYTDANGKGDATVNAEEALAIMLEKLDIIHGMFA
                     KTATSAGFDYTGFEAHAQKLLVPVANYVLGLEDGKKRFLDNVLAITKAWSLCCTLDAA
                     QPYKLEIAFLSAVKAAISKFTSVDKKISQAEKNSALKQILDNAVIANGVDDIFTLAGL
                     DKPNIGLLSDEFLQEVRDMPYRNLAVELLEKLINDGIQSRTKNNVVQERKYSERLQAV
                     LIKYNNRAIETAQVIEELIQMAKDFQSAMERDDALGLNPDEIAFYDALAENESAVREL
                     GDEVLKKLAIEVTLKLRQSTTVDWQVRESVRARLRILVRQTLRRYKYPPDKTPGTVEL
                     ILKQAEVISNHWTM"
     gene            25444..25941
                     /locus_tag="LAF92_000707"
     CDS             25444..25941
                     /locus_tag="LAF92_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789763.1"
                     /translation="MNTAQLPKKANIYKAMFYFFLNKEKFITLGAINKMAFELEKDEY
                     KRKLEAGKYSLNPDDHKHSLEEYCDDLRKSILASVLIMLGVLFIVIAIGFYSGKFRII
                     YPLEWKDAVSATGIFILSWSTLFQLGWRNASWKGCRLDELVGLAIFRHVFIFGLFLSL
                     LCFAI"
     gene            complement(26630..27508)
                     /locus_tag="LAF92_000708"
     CDS             complement(26630..27508)
                     /locus_tag="LAF92_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI9789764.1"
                     /translation="MTSDKALKQAISNITIWRKGEQRAPHKPLLLLHVLSHYRQGHGR
                     LFNYGSEIYEPLLDLLERYGPQRRDQRPDMPFWRLKGDGFWELQNAQLCSTSGSRQPP
                     RRELIEYNVAGGFDADNFALVAKNHRLIDTLAQQLLEAHFPASIQEEIADEMGFDIRT
                     SLRQRDPKFRQMVLRAYNYQCAICGFNMRHDNAPIALEAAHIRWKQHHGPCEVPNGLA
                     LCAIHHKAFDRGSIGLDENMRVVVSGAVNGGGVVQRLFWDFAGKAIALPPVKENYPGE
                     QFVEWHRKEVFRGEGI"
     gene            complement(27724..28992)
                     /locus_tag="LAF92_000709"
     CDS             complement(27724..28992)
                     /locus_tag="LAF92_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBI9789765.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLVDARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWFVTNQKWSEDHANRVKKSLEDNIFPAIGTRNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     SKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPRKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANRERAITPFDYAKINRGNGE"
     gene            complement(29565..31898)
                     /locus_tag="LAF92_000710"
     CDS             complement(29565..31898)
                     /locus_tag="LAF92_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBI9789766.1"
                     /translation="MSGMKVSQAVKAARGHWARILPALGVNILKNCHQPCPVCAGKDR
                     FRFDDLEGRGTWFCNQCGAGDGLALVSKVLNVDISEAADRIHGLTHSLSSASSGVWTS
                     TADTDGGKQAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSGHKTGGVMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRMWIAEGYAT
                     ALTIHHLTGEAVMVAFSSVNFLSLARVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ACQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSHYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPARHLIGFRNGVLDTRTGLFSPHCKENWLRTVCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
     gene            complement(31913..32236)
                     /locus_tag="LAF92_000711"
     CDS             complement(31913..32236)
                     /locus_tag="LAF92_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HBI9789767.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSVCHQQNRHPQLTLDEIEAA
                     IACELEGFYLRQHGQTKGMEIACALLSDLMESGTLMPCPALSQLGIAVMDELCVRHII
                     KPVLH"
     gene            complement(32233..32457)
                     /locus_tag="LAF92_000712"
     CDS             complement(32233..32457)
                     /locus_tag="LAF92_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789768.1"
                     /translation="MNHAAISYDDIVRLKHLRNVGEFVTGMAVLQDCHEKPAGAQCEQ
                     LASLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            complement(32457..33002)
                     /locus_tag="LAF92_000713"
     CDS             complement(32457..33002)
                     /locus_tag="LAF92_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBI9789769.1"
                     /translation="MMPERINPHFSGLHPAVISRYSFSAAAKSAAGRRNPSYLLATPD
                     APCVFFYVVAQAHPFFGLRCLYLSPCQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHER
                     RNSGGGIYRDLLEVALMATILAPSHPQFIFVFAAIRRAERKPRISMLRIVASNEHHAR
                     LSLVHEYVLCLAARLPVREVM"
     gene            complement(33002..33262)
                     /locus_tag="LAF92_000714"
     CDS             complement(33002..33262)
                     /locus_tag="LAF92_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBI9789770.1"
                     /translation="MHDISVSAPAPAAPLFPVLNQHERFLRLPEVMHLCGLSRSTIYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMAERISARGRGCDA"
     gene            34176..34928
                     /locus_tag="LAF92_000715"
     CDS             34176..34928
                     /locus_tag="LAF92_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HBI9789771.1"
                     /translation="MTTVNQISDANITSSLPPKIREAVEKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            34925..35479
                     /locus_tag="LAF92_000716"
     CDS             34925..35479
                     /locus_tag="LAF92_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HBI9789772.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEELRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEVRTPVISAAYKSIIDETGLTVDASMYADAS
                     ASFTPAQHKVFLARLNRLNEKGGY"
     gene            35481..35753
                     /locus_tag="LAF92_000717"
     CDS             35481..35753
                     /locus_tag="LAF92_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HBI9789773.1"
                     /translation="MALKCPECGTVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPWRNEQESDKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            36100..37086
                     /locus_tag="LAF92_000718"
     CDS             36100..37086
                     /locus_tag="LAF92_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI9789774.1"
                     /translation="MARGELMKKLLASYGNDDEFRSVAERIINEEEKKNNKVLANSLR
                     RTLENSRAIGSNYSLKGLERFSPMNEATNDFVERVEPSHFHKDLVLSFDNVRILKGLI
                     EEYRFAEAIRKNGLSLRTKLLFCGPPGCGKTLTAEVFASTLGLPMFIVKLDKLMSSYL
                     GETATNIRSIFEFANNKPCVLFLDEFDAIARSREDSNEHNELKRVVNSLLLFIERLSP
                     KGFMIAATNLDKSIDPAVWRRFDEVIWFKNPDKGMIRRFLKSRLKNIKYNFNPDDYVY
                     KLEKMSFSSLERVLNQAIKSMIIGKHKVLNERYFVDAINDEHRRNSGVEQMR"
     gene            37104..39461
                     /locus_tag="LAF92_000719"
     CDS             37104..39461
                     /locus_tag="LAF92_000719"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBI9789775.1"
                     /translation="MAEYEHLRLVKIPQQLERRKKPGYGSAPKRNSQERTIHGTSLIH
                     QIDSTVAYVRSKRIPGNIDPSLIMKVKYDGSAADEDWLRLGLQVLSHDDDKTLVLFSS
                     NDSLADLKDRINAYSGPIPAKQKNPSYNNFVGNLISIETITSTDRIGIRFKESGFNAI
                     DDFDEMENYIVDIELWDFGGRIEREAKIETIKNLIVNNGGDFLDSYIGPSISLLRAQC
                     NISALKLVLEIPEVYTIDLPPMPDMETEKYFKFELDDTPQPIDAYEDLPIIGIIDSGL
                     NNHPFLDNVIVGTVSYLNDAGQDSWGHGTSVAGVAVFGDLRHQLAQNQLIRYARICSA
                     KVVDDEGRFPDDVVIPKLMSQAIRQLHHNFNCRIFNISLGDPKAPFKGDRNGQWASIL
                     DDLARDLDILIIVSAGNGSSWGAEPEDAISEYPNYLIEKRNRFLEPAGAVNVLTVGSL
                     SHGDGLPPDLAEYVGVIPTARMDEPSIFTRSGPGILGMCKPDLVDYGGTALYDTAIHN
                     IRPGGDIPESGILSLNNKYLDSLFKASSGTSVAAPMVANKAALLLKQIPNSTANLLRA
                     LLVNASSHPLENQKRINLLDGKSKNDIYGKGLVRVKNLISSEDNRVVLYSQDVLQPDY
                     FAVYEIPIPDNFKNTKGKKNISITLAFDPPVRHTRKDYLGCEMNFRLIRGKTADDVFN
                     FFKVKEEGDAISEMPKKYNCDLKPGANSRDFATIQSASVSYDNAIENYGNTYHLVVRC
                     QAGWADFIQQQRYAIVVELTHTENVNIYQTIQQRVQPRVQNRNRI"
     gene            complement(39506..40762)
                     /locus_tag="LAF92_000720"
     CDS             complement(39506..40762)
                     /locus_tag="LAF92_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBI9789776.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSTKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRIMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMILLEQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNSVRAAYIHTSEHLDERRL
                     MVQWWSDYIDATKKQMITPYEFAKKT"
     gene            complement(40926..41010)
                     /locus_tag="LAF92_000721"
     tRNA            complement(40926..41010)
                     /locus_tag="LAF92_000721"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40974..40976),aa:Leu,seq:caa)
     gene            41245..41454
                     /locus_tag="LAF92_000722"
     CDS             41245..41454
                     /locus_tag="LAF92_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBI9789777.1"
                     /translation="MLAGHKAIGRVAALGSAAQNKGLQVGQRTAHGSGYCDTFIRCNQ
                     LNFRQGAAPTIMNRGGFAGSLLPSL"
     gene            41537..43039
                     /locus_tag="LAF92_000723"
     CDS             41537..43039
                     /locus_tag="LAF92_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBI9789778.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(43158..44240)
                     /gene="lptG"
                     /locus_tag="LAF92_000724"
     CDS             complement(43158..44240)
                     /gene="lptG"
                     /locus_tag="LAF92_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBI9789779.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(44240..45340)
                     /gene="lptF"
                     /locus_tag="LAF92_000725"
     CDS             complement(44240..45340)
                     /gene="lptF"
                     /locus_tag="LAF92_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBI9789780.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            45607..47118
                     /gene="pepA"
                     /locus_tag="LAF92_000726"
     CDS             45607..47118
                     /gene="pepA"
                     /locus_tag="LAF92_000726"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBI9789781.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            47252..47695
                     /gene="holC"
                     /locus_tag="LAF92_000727"
     CDS             47252..47695
                     /gene="holC"
                     /locus_tag="LAF92_000727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBI9789782.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            47695..50550
                     /gene="valS"
                     /locus_tag="LAF92_000728"
     CDS             47695..50550
                     /gene="valS"
                     /locus_tag="LAF92_000728"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBI9789783.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(50605..51801)
                     /locus_tag="LAF92_000729"
     CDS             complement(50605..51801)
                     /locus_tag="LAF92_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBI9789784.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIIDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            51994..52497
                     /locus_tag="LAF92_000730"
                     /pseudo
     CDS             51994..52497
                     /locus_tag="LAF92_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(52543..52959)
                     /gene="rraB"
                     /locus_tag="LAF92_000731"
     CDS             complement(52543..52959)
                     /gene="rraB"
                     /locus_tag="LAF92_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBI9789785.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            53121..54125
                     /gene="argF"
                     /locus_tag="LAF92_000732"
     CDS             53121..54125
                     /gene="argF"
                     /locus_tag="LAF92_000732"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBI9789786.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRTLAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(54226..54456)
                     /locus_tag="LAF92_000733"
     CDS             complement(54226..54456)
                     /locus_tag="LAF92_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789787.1"
                     /translation="MKQPDFAKWYFYQLLKDYEGEQLYLNELGYVYGNEEKTNEIVKN
                     NPGYVVKIFEEKMVNELKIRTRMMKILRKIYV"
     gene            complement(54443..55786)
                     /locus_tag="LAF92_000734"
     CDS             complement(54443..55786)
                     /locus_tag="LAF92_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBI9789788.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKMFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFPQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNISQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDN
                     EINDIKVNLSHILIASLDDAKVNLTPVIDSILEIFLKFPYINDVRILDWCFNKRMQYF
                     GDSEKIKYACSVINHIDFSRDQSKDFSCDQSKIKIAETLFFNLDKEHYKNSRKLQELI
                     WDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEVPVSILVNDIFFEGFFHEAT"
     gene            complement(55909..56361)
                     /gene="tabA"
                     /locus_tag="LAF92_000735"
     CDS             complement(55909..56361)
                     /gene="tabA"
                     /locus_tag="LAF92_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBI9789789.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(56506..57099)
                     /locus_tag="LAF92_000736"
     CDS             complement(56506..57099)
                     /locus_tag="LAF92_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI9789790.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            57170..57883
                     /gene="bdcA"
                     /locus_tag="LAF92_000737"
     CDS             57170..57883
                     /gene="bdcA"
                     /locus_tag="LAF92_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI9789791.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            58014..58409
                     /locus_tag="LAF92_000738"
     CDS             58014..58409
                     /locus_tag="LAF92_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBI9789792.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            58690..58824
                     /gene="pyrL"
                     /locus_tag="LAF92_000739"
     CDS             58690..58824
                     /gene="pyrL"
                     /locus_tag="LAF92_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBI9789793.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            58828..59763
                     /gene="pyrB"
                     /locus_tag="LAF92_000740"
     CDS             58828..59763
                     /gene="pyrB"
                     /locus_tag="LAF92_000740"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBI9789794.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            59776..60237
                     /gene="pyrI"
                     /locus_tag="LAF92_000741"
     CDS             59776..60237
                     /gene="pyrI"
                     /locus_tag="LAF92_000741"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBI9789795.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            60310..60696
                     /gene="ridA"
                     /locus_tag="LAF92_000742"
     CDS             60310..60696
                     /gene="ridA"
                     /locus_tag="LAF92_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBI9789796.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
BASE COUNT        16965 a        14200 c        14012 g        15576 t
ORIGIN      
        1 attatttctt tcccataatc cggcaaaacg agcagcatta ctggcggtat aacgcacagt
       61 atggcgaata tttcgatgcc cgagataatc ctgaattaaa cgagtatctg caccacgctc
      121 cgccagttca taaccgcaag catgccttaa catatgagga tgagtctgcg ttacggttcc
      181 agcttcaata ccggcatcgc gaataatgcg ataggcctgc tggcgagaaa gccgactccc
      241 gcggcgagaa ataaatatag cgtcggtccg gtcagcgcct ttccagttag cacgttcctg
      301 ggtccagcgt tccacggctt cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc
      361 gttcttcagt cggcgaatat ttattcgacc ttcattaagg tcaaggtcct gataatgcag
      421 atcaagcagt tcactaatac gcatcccatg ccgatatgcc aacagaataa gacaataatc
      481 tctggctccc gttgccccgt aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt
      541 aagataacga cgtttactca caatagtagt actcctaact gagatatatt tgaatgaata
      601 cctataggaa acctcaatcg gtcaaaatta gcccaataga taaaaagata accacacacg
      661 aagtgatgtg gctatcagtc aattacatta aatatcatac aaaaataaaa tatcactgat
      721 gtgacaacat ctattttcat tgatttgatt caatataaca atagattgca gtggattaac
      781 ttccagttgc ttaaaaaata aagaaaaaat gtgaaaacag ataaatttaa gtgatgtgta
      841 aatttatgaa tgattttgtt tatttatttt gaagaacata aaccattgta ttttgtatgg
      901 atatggttaa cttaatgttt attatggtaa ttaagaatca cacttacaat aacatcaatt
      961 aataaatggt taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc
     1021 tggtatctca actatctcta taaaacagcg tgacgctgtc gtcctctggc tctatcccag
     1081 atgccgtaaa aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga
     1141 tgcccaagat aatcctggat aagtcgcgta tctattccca tattcgccaa agcaaaacca
     1201 cacgaatggc gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccggaa
     1261 gtcgagataa tatggtaaaa ctgttgccga gaaagcggat ttcccttacg agataaaaat
     1321 acccactcgc tctcagcatg cgggtacgaa gtacggatac tcaaccagtt ttttaaagcc
     1381 tgaacttctt tgttcaatag cggatgcgtt gttgaaaagc ctttttttaa tcgatggata
     1441 tatatacact ttgccttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc
     1501 cggaaaccat gaataaaaca aagcaaagtc agacaataat tacgtgctgc atgaggcccg
     1561 gtatttgctg ctttaaggag tgattcgatt tcactatggg tcaggaagtt cctttttttg
     1621 ttatcagcct tattcttcat cgtttttccc ttataattac agacgcgcac tagctgtgct
     1681 gggttagcaa taatccaaca tttttatcct gattatgttt ataaaagcga acgtttgctt
     1741 aataactaac gtaagtggac cagttcttct gagtgaactt aaatggagta gcaacagtta
     1801 attataataa cgttaccata caaacgccat gctcttgcat gctatgtacc tttatatatt
     1861 tatcaatcgg atacgaatct aataataacc ctcatcatta atgcaactaa tcttatctat
     1921 atatcatgtg atatgttctg taacaagtaa aatcaacata aaaaattcag caaaatttaa
     1981 tattcgttca actagccgtc tcacatatga cattattagt caactccatt tgccacaaaa
     2041 tggctatttc atggagcgca gtaatttctt agtggctatt atcatgctaa ttcttaggtc
     2101 cctagcgatt attcctggct gattcgtcag cttacgccat gagattgtta cattcctttt
     2161 tatcctggag atatggggaa cgcaggagat gattttttca tctacattcg ttggtaaata
     2221 tagctttggt cactacgact taaaccttcc tcaatcacag ccattcgccg ggcgatacgg
     2281 caaacaaact aagtgaaaca tcatcctgaa aacaccaaca tcaacaagcc tctccagatc
     2341 gacttcagaa gtgaccagtt acaagccaca aaccaggaaa catatttcat gatgagaatt
     2401 atgctcagcc tgatggcggg taaacactga ttttcaccac gctcactgag ttagcttgca
     2461 aatctttcct tgtttatact ttaaataaat tacttataaa acagataatt aaacaacaat
     2521 ctttggtttg tgaaggcctc cgcaagtgtt cgtctggctt cacatggcat cttcttctta
     2581 gaaaaatatc gacatatttt gtgacacgaa ttgcaaatct ggttttgttg tatggattgc
     2641 gtgatttttg atctggtata acaggtataa aggtgcacca cgatagtcaa tgagactggg
     2701 catctcgtaa tctatggcaa acatcacttc agttctttct catcgggtga tgaaaacgca
     2761 cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactccggg
     2821 taaaatggat tgagtaagtg atatagctta cgaacattca aatcaattaa acatcagaag
     2881 agattttata ctcaggtatt taatctggct ctctgtttat ttaaataatg tggaaagaga
     2941 tttttcacag gagacattat acaaaaaata taaaatacag ctaccggttg ccaaagacac
     3001 tataagcctg gcaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta
     3061 ttgctttctg tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc
     3121 gtattattac tgtgcttttc gtccccatta atttctcagg ctgcgacact ggacgtacgt
     3181 ggtggatatc gtagtggaag ccacgcctat gagactcgac tcaaagtcag tgagggatgg
     3241 caaaatggat ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa
     3301 aaggaaaatg ccgcactcaa tgatgttcag gttgaagtta attacgcgat taaacttgat
     3361 gatcaatgga cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac
     3421 ggaccctacg taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat
     3481 cgttacgact ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac
     3541 gttcatcgtt gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg
     3601 caaacgacgc tatacagcaa acagaacgat tatcgctatg caaaccataa gaaatgggcg
     3661 acggaaaatg catttgttct acaataccat atgacgcccg atattacgcc atacatagaa
     3721 tatgactacc tggaccgtca gggtgtttac aacggcagag ataatttatc ggaaaacagt
     3781 tatcgcattg gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac
     3841 aataacggcg cttgctatca tgatggcttc atttgccgca aacgcgtctg tattacctga
     3901 aactcctgtg ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg
     3961 tttaggtagc gcaggtacgg catggtacaa gctggataca caggccaaag ataaaaaatg
     4021 gacagcgtta gctgcattcc ctggtggacc aagagatcaa gcaacctctg catttattga
     4081 tggcaatctg tatgtgtttg gcggcattgg caaaaacagc gagggcttga ctcaggtatt
     4141 taatgacgta cacaaataca accccaaaac caatagctgg gttaaattga tgtcgcacgc
     4201 gccgatgggc atggcgggcc atgtgacttt tgtacacaac ggcaaggctt atgttactgg
     4261 cggtgttaac cagaatatct tcaatggcta ttttgaagat ctcaacgaag ctggaaaaga
     4321 ttcaaccgct atagataaaa tcaacgccca ctactttgac aaaaaagcag aagattattt
     4381 cttcaataag tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga
     4441 atctccctgg tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct
     4501 tattaatggc gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgacttcac
     4561 cggtgataat ttaaaatgga ataagcttgc tcccgtcgca tcaccagatg gcgtcgctgg
     4621 cggttttgcg gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg
     4681 ttcacgagaa aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata
     4741 tagcactgat attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca
     4801 aggtcgggcc tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga
     4861 aactgcaggc ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt
     4921 cacagtacaa aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttaccat
     4981 ttaaattacg cgaaaattat atggacacaa taatatcgcc cgattattac tatgttctta
     5041 ccgttgctgg tcagtctaat gctatggcgt atggcgaagg actgccatta ccggacaggg
     5101 aagatgcgcc tcatcccaga attaaacaat tagcaagatt tgcgcatacg catcccggag
     5161 gcccgtcatg tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg
     5221 atatgcaggg ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg
     5281 gccaggcact gcatattgca cggaaattac tgcccttcat tcctgataat gcagggattc
     5341 tcatcgttcc gtgttgccgt ggcggatcgg cttttaccgc aggcagcgaa gggacatatt
     5401 cagaacggca cggagccagc catgatgctt gtcgttgggg aacggatact ccgctgtacc
     5461 aggatttagt cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg
     5521 gcgtatgctg gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc
     5581 aacactttaa tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc
     5641 ttaataatat tactgacgcg ccgtggtttt gcggcgatac cacctggtac tggaaagaaa
     5701 atttcccgca ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata
     5761 ttattttcgt cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag
     5821 acccggacga tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga
     5881 cgacggcact gcgaagcagt catttcagca cggcagcccg tcggggggtt atttctgaca
     5941 ggtttgtaga agcaattttg cagttttggc gcgaaaagtg agcgttcatt aatttgattt
     6001 attcgcatcc atcaacgctt ttaactcctc cggcgtaaaa ggtggtttgc gccggtgcag
     6061 catcgcatgg caattagggt acacaggcag caaaaaaaca aatgagatca aagtaatata
     6121 tcttcacttg caggcttgaa cagccgaaaa tttgcgagca tgatcgcaat tatttcactg
     6181 ccagggatat caatggcttc cagtaaatcg ctacagcagg caattgccaa cataaaaatc
     6241 tggcacaaag gtgaacagcg cgcgccgcat aagccattgt tattgctata cgtattagcg
     6301 ggatacctga atggacatcc acgccttttc gattattgta gtggaacagt ctacgaggat
     6361 gcagtttgaa gtgagtaagt aaaggttgct ggaaacgatc aatgccgcca tacaatgata
     6421 tttgaggatc accaatgagt aataaaaact acgaaagtca ccgcaaagca atagttagta
     6481 aaggtatacc acccactcta ttaaataggc tcaccaattc ggatgttcag gtgatcaata
     6541 ccttcttaac acgagtgagc aaactggaac tatctcaaca agagaaagac tggatcataa
     6601 aaatcatctc tatggtttag ataatttcag aatttcaagt aaagacaatg ctgcatatca
     6661 agcattcagg atggctagcg ctaagctcct aaacctggag taagcctcat cccatgtgat
     6721 ctttcgctct ccatacacca ttggcgaaac aaactgctga tagcgcatct cgtagagagg
     6781 attgttcgcc aactcatcca gtcccatttt tagctcatca actggagacg cgcaaaactg
     6841 cgggtactgg ttgccgtaac gctgaatgtc ctgctcaata gtttgttgaa caaaatcaac
     6901 gagctgactc atatcaacat gactcgtctc aacaatacag tttgtatcgt agatatgacg
     6961 taccagcgat gcatcatcaa atcgctcaac atctcgcaca actgccgcag tacgacgtaa
     7021 catcgccacc agcttttctg cctgtgtgct aatgatagtt gcgcatggaa acgctgaaac
     7081 aatagccgct tttcccgtga tccctgttac cagtgaatga atatcccgta attcagaagg
     7141 ctccagtaac ttcgcttcca tcaattccag ccggataaaa ggacgcaaac aaggtgcctg
     7201 caccactcgc tgtggataac gaacaggtat gtcattataa cgatattcat cccgcgtcac
     7261 ttttgggtaa tcatcatcaa aattgaacac acccgatgaa gctattgcac tggaaactgc
     7321 ctgaacaatt tctttacggg tattttttcg ctgagtgcgg gaaagtgcct gaaatgcatc
     7381 cttaggcacc agtttgatat ccacatcttc cgacatgcga ttaagtgcga cacttgattt
     7441 tgccagagcg gttcctccgg caaacaccaa ttgatgagta tcgaacacca gaggtttaag
     7501 caattgcaaa agctcaacga tatagtgatc tttttccaca attgcgacag actcaatacc
     7561 aagcgcgtca gccacttcag aaaaaagagt ggttaaatca tccatttaat ctgctgttcc
     7621 ctacagataa tacgcgttta aaacgccggg gggtttttat ctcgggtgaa gctgggatct
     7681 gcgtagaagt accgctattg tatgcctgcg ttgcgccagc aaaacgatag tgtactttca
     7741 ggcgttccag cgtttcgaca atcaccgctg aagagcctcc gggagccgct ggcattacct
     7801 tgcctgtaat accgttccgc cgtgctttgg tatacacgcc ataaccaacc ttcaacagtt
     7861 gcccttccct gacaagatcg cgcaataccc gaccaacctg atcgtagcct gcaatatctt
     7921 taaagtcgtc acgagtgaat acgtaacgtt tagaacgttt cagtcgtgac tgaatgcgtg
     7981 cttttatcgt catattcgcc tcctgagtca ttgcataaag agtagcaaaa atacgacatt
     8041 ttagatagca aacatacgac attcattttg ctgataacgc tgacggaaag tgcttcgcca
     8101 cgatatcatt catcttctcc accagatcca aacgtttgaa agtgatatgc ccactgccct
     8161 tctgaaagta gcgaatagtg aataactcat cttcgaaaat ctgctgatgc ggattatcgc
     8221 gcacaaaatc cataaaccgg atggagacat catgccggtt atcgggaatg gtcttcccat
     8281 ccagcaggta gaacatccgt tccagatcgg caagctgatc tcgccgccag ccccagttca
     8341 ggctgtagcc ccatctgtca tgcttcacca gattgttaac gataatcttc ttaccgaaat
     8401 aacaaggatt attggttttg tagtcccagc tcaatccctt aaatacattg ataatcccac
     8461 gttcgaaaac gtcctgctta ttgtggtgaa gttgttcaaa ggtactcagg atgttggctt
     8521 cgctaatcgc agggagatcg ccctcctcca ggttctttgc ccactgactg cgagcttcgg
     8581 catccatcag agtcaacatc ccagacttca gcatgagatc gcgccagatg ctgcgatcga
     8641 tatttcgggt gatcaccggc attgctttgt cggttggctg catcagccag cagtcaaagg
     8701 catatccatt ccgcattgcc caggttccag ccacatcacc gccaatactg gatgtcaggc
     8761 tggagatatc atgaagtttt tggattaacg tcgctatctg gttcagtgca ctatcacgac
     8821 ccgtcacgat acgctcaata ctggtcgagc aaattagctc gttatgatcg gttaagactt
     8881 cagcttctgt ttgcataatc ttttgtccgt ataaaagcaa atgcgccagc catgaacagc
     8941 aggcgcatta tgaagggagt tatgagagaa gtaattttgg cgatgtaaat cgggaaggag
     9001 actcgcagat tcaccgtgcc gtggtgttat ggcgtgttat ggcagcggcg cagcagacct
     9061 gtagcctgcc gcgcccgtaa aatatcgacc gcacgaagat aaggcgtctg ttcctgccag
     9121 ctaaatcctt tcctgtcgat acggatcaac tcgtacttct ccaccagaaa attgacggca
     9181 tcgaccagag tgatcccggc gtctatgtgt tcctgaatca ccttttcatt gttgaatggt
     9241 gtgtcattaa gattcagccc gtagtgctgc tccagcaggc gggccagtaa tgtttgccag
     9301 accacgacag gcgacagata tgacgtcgtc gcccgcagcg tcgttgcagg aataattttc
     9361 ataacgatgt tccgtattaa ggaggttaaa actcagcgtt gagtgggata aatagcgata
     9421 tagacgtaac cacaggaacc cagcgtatcg gcttcgcagg ttagtccttt ggcgtatagc
     9481 gtgacggtac gttgttggca gggggagagt tcaccgtcag ccagtttcag ctccagttgt
     9541 ttcatcagca ccggaaaagc ctggtcgata tgcagcagat ccggggagct gaatttcccg
     9601 gtgattgcgg cccgatcagc caggtaatgc aggttatgtc tttcctgtac cagtcgagca
     9661 gtgaagcgtg gtgttacggt acagggaagc ccccattctt gttgcatgac cttgtccttt
     9721 tgcagcatta cagccagccc cgttcggcaa aagagagaac atcacccgcc ccaataatga
     9781 aatgatccag caccctcact ttcaccaaat ccagtgtctt aacaatattc gttgtaataa
     9841 gtctgtcggc ccgactgggt tccgccacgc cggagggatg gttatgcgca aggatcactg
     9901 ctgcggcatt gtaccgtagc gcagatttca caatctcacg gggatgtact tctgtactgt
     9961 taatcgtgcc gagaaatagc gtttcgtgtt ccagcagtcg gttctggtta tcgaggtaga
    10021 gcacaacaaa catttcccgc tcctgtcgtg ccagatgcag ttgcagccag gtttttgtgg
    10081 cgtcagagga aagaaaagat tcgccaggat tgcgttgata tttctccagc agacgcatcg
    10141 cacggcggat aacgcgttgt tcactcaccg aaaataccgg tgttagtatc agtttagaca
    10201 tcgtatttcc tttactatta atagcgttgt ttatgaggcc agttgcaaca tgttttcagc
    10261 catcacccac agggcgcggt tgagtttgat atcgccgtcg atgcctttaa tggcgcgggt
    10321 gtgcgtgcga ccaccttttg ccgtgcgccc gtttaacccg cctttgatca ggttctcctg
    10381 aatacgctgg taggtcgtcc acagatcgcc ggattcatcc tgccagcggc gcggagccag
    10441 caactgggat tccgtcactg gctggtgctc ttcgccaaag cggtaagtca gcgccgcctt
    10501 tgccagcgcc tgttgcgcag ggggtggcag catcagcgac tgcatggcat cgcgtttctc
    10561 ttcaacctgg tcaaaaacac tgaggacttc ataagcgcct tcaatcactt tctccaccac
    10621 attgccttta tgcggtaccc gtacttcacc aaacgactcg ccacagacca gcccgttaca
    10681 acaaacagaa cggaacagcc ctggcagcat ctgataagaa ctggaaccgt catggctgtt
    10741 gagcagaata atttccggca cctggttgcg agtgatctgg tcttcccggc gcagatgcag
    10801 catgtgtttt gtatgctcac gtttacccac atcacgaacg cgggtctggc aggcaaagaa
    10861 aggatgaaat ccttcacgct ggaggttatc gagcagagta atggtgggaa tataggtata
    10921 gtgttcgctg cgtgattcgt gcttattttc cccaaatacg ctggggacat actgcatcat
    10981 ttcgtcatgc gttaatgggc gatcgcgacg gatttgattt acgtgaccaa agcggctggc
    11041 taatttcatg gtgaattcct taataaagaa tataaaaaac aggccgcatc ttctgaaaaa
    11101 ttgcggctaa tttaatgcgt gattaaaacg gacggtagta taggtgggat aaaataacag
    11161 gtacgtgcag atattaaatt acattccggc agtgcatata tgaaagtgat atagtgctga
    11221 aataatacat atctgaattc tcccggtgat agtcaccggg agaatttaag tttgaaatga
    11281 cacctcaacc acgggataat cttgtttcta ttccctgaac tctttcgtct attaaccttt
    11341 caataacaga tattttatct gacttgtaaa atttaaccag aaaatccaga cgctctttat
    11401 gcgtatcact gatagactcc cgttcccgtt tttcagaaag tttacgtctg aagttttttt
    11461 ggtaaaacgc tttatttatt ttctttaccg ctttctgctg gaggtcatca tccgctgacc
    11521 ataaatccag catggcataa atggcgtgat aggtttctat actattcagt ggttccggga
    11581 tctgaacggg gtccaactgg cttcctgtat tactaccgtg ctctttctgg aatttattaa
    11641 tgtaattata agcccatata cagacttcgt cattatcctc attaagccag cgtaatttta
    11701 ttctctgatg ttcatttgcc ctccagaata caagcctctc atatagccac tgtctcatca
    11761 acggtgcctg attagtctca atattaatat agtccagtaa tgcaacgatg attgagtatc
    11821 tttcgctatg acttgcagga gaatctgtta ttccggcttt ctgataccag ttagtgttta
    11881 aatttgtgcc cagcttttcg ttaattgact ccatacagcg gtaatcaaga cacatcacgc
    11941 cccatagcca gaacgtcgct ctttcatttt cttttaacca actaaaatat ttctccggca
    12001 gaagtgtcgt attaattgcc agctccagtg agcgaataaa attacttcgc tgaatggggt
    12061 cgggataccc ggcctcgagg gctgtattaa taattctctc cgaaagagat gtatctggat
    12121 tgttcccgtc aaacgcaaat tgatttgctc catgaatgtt tttcctgatg taccagaaga
    12181 ggaacctgct ggttcggatg tccttcactg ctattgatga gcgcacatta acggccattg
    12241 ttctacctcg tcatccttgt taaatcagca aaaagcaata gcgtaattat gacgtgtttt
    12301 cgaagtaaaa ccgtagcaat aaatcaataa aagtggaaaa tacgtagtaa tatcgtgtta
    12361 ttccatagta atagagtacc attaacgtag ttttttattt atcaccatac tcacagcttc
    12421 cgcccgtaga aaacggctgg atagtcttaa atttttccac gtatagccct ggtggcttta
    12481 cgggtgaaac ctcccaggat aacgagttga catctggaag atgtcattta tgaccgcaac
    12541 tcatagatta gatcaatgta ttaattataa cttaagagtg aaattcttta tcttcaggaa
    12601 cagataatta actacgaaca ccagtaaata ccgggcttct actcttttta tccccctttc
    12661 tttatgaatc aacattgccg gtattaatat tatgtttatc actcattcat tagattcagc
    12721 atatagaggg atgacaccag agacagggaa aagtaatcca gatcggcata gctcagtgaa
    12781 aagttactac agcacttatg gatatgtaac caaccacaag gaactgaagc atgaaactga
    12841 atgaacgtta tggcaagcct aatgcacact atatgaaacg tctggaagag attactcgct
    12901 atgcgatgga tgattacgcc agaatactgg ctttcagagt ggatttatta ctgccagtgg
    12961 gtaatagcca taaagggtat gattcagcgg ttattacccg ttttatagcc tcactgaaag
    13021 cacagatagc cgcatattta caacgccgca ggagagacgg taaacgtgtt tacccctgta
    13081 agctgcgata tgcatgggta agagaatttg gcgaactgaa tggcaacaag cattatcaca
    13141 tgttgttgct ggttaatcgg gaagtattca ggaacaccat gctgaatacg gggaattcag
    13201 cgcaacctcg cggattgctg gtgggaatgg tggcgcgggc atgggctgtg gcgcttaggc
    13261 gtaccggaga tatggcaaat aaagtcccct ttcatttacc aggcgtctct tatgaacttg
    13321 atcgcagaaa tggcacaaat acggactttt tccgcagcta catataccac atcagttatc
    13381 tggcgaagga gtacaccaaa aaccacgatg acggtgaacg taactttggt tgtagtcagg
    13441 gataagtaat ttattaccgg agaagtgatt gtaatggcgg agtatgcaac atcatcttaa
    13501 atcaatgggt tagtgtcgct gctcctgtct tttatcaacg taaagacaga aggacaatcg
    13561 tcatgaacaa tcaaccaaat accgatactg acctgatgaa tgataaactg gtgacgatgg
    13621 catttattac cacgtttact ggcctgaccg acaaatggtt ttataaactg attagtgaag
    13681 gcaagtttcc aaagcctatc aaactggggc gtagttcccg ctggcgggaa agtgaagtta
    13741 aacgctggct gacggaacgt attgcagaat cccgctatta atccccctga cactatctct
    13801 acacgcttta cccataacgc tatatcagtg agcgcctgac agggccgttg ctgtgcatct
    13861 atcgacgcat aacttcatta tgcccggatg aaatacgttt cgtttaacat tgtcgggaaa
    13921 taattaaccg gagaatagcc tgcttattta gcaggaattc cccggataca gccacagata
    13981 aaattaggaa ttaaatgtcc agctacccgt atcggtcaca atgtcagctt caatgatacg
    14041 gtcacacttc atatatttga acgtagccac atcaccgaat ttatatttgg ctggcagggt
    14101 tctgttaccg acgctctcca ttttacagtt ccctctgttt acccgaatgg atttcagcac
    14161 cacgttgtca tcagtgatag tgagctgaat aacaccatat ctggtattcc agccttcatt
    14221 gctgacagag ctgccgatct gaactttaaa tggtggtgtc atagattgct gtgcctgctg
    14281 ttgttgagag ggagcaggtt tattagcctg ctggctacca acgccattaa tgccattctg
    14341 aaggaggctc ctggaaacct taatggtacc cggcttgcat gatactgtgc tgatttcctg
    14401 ccccattgca ttcatggcag tgtaataagc atcacctgta ccatcatatt ctgagtaatt
    14461 gactgcaacc gcgccttcga tatcttcatt ttctccacgt aatgtgaaag aagcggttcc
    14521 tgctcccgga ttagccgcca gagtctgttt cgcttcgccg gaaggaatat ccagtttaat
    14581 aacagagccg ctctcttcgg agtcaccgcc tggattttca aaactgatat aaacggtatt
    14641 gctaacgtgg ctgagtgtca tttgctcacc gttatccagc agacattgca ggtcgtaggc
    14701 cccgactgca taagcaggtt ggaatgcaaa taaagataca aacaatgcag gtataatgat
    14761 tggtttcatt aagcacttcc ctatccaaaa aaatggtccc aggcattacc agcccactcg
    14821 ataagtatgt ctttaccctt acgggtaagc tctttgacag gctttggtaa cggaaaagaa
    14881 tcaataacgt cgtcgagtat cccggagatt acgctgccga agccgttttt tgcttccgtc
    14941 ttgacctctt cggttttgaa ctcatcctta aactgcgaag ccgtgctact tttagcccga
    15001 tcaggcaaag cccgaataat ggtctctacc agacgaagaa tgttgtagcc ctcatcggcg
    15061 gagacaggtg tcaccggata gtgcgtaaag ccaaatcgtt cagtaatgta ggcttctttc
    15121 ttttcgatgt tgatgcgctg tgcaggagat ggctgatgca gaagagtatc ccactcgcgg
    15181 aatggctcaa ttttatcaac ctggttaagg acgaataaaa cgcgctcacc cccaccagca
    15241 gggagcagaa cattgttgta gaaatgctca tccgcagaaa aggcccgatc gtctcctttt
    15301 attatccaca ggataagatc cagggaggga agcaaattgc ggtataaatc ttcatactcc
    15361 ttatcccgct gggcattctc accgacacca ggtatatcaa tgatctttag cgaatgtttg
    15421 ccgaagcgta tacgcaattc ctgaacttcg cgagtacatg cctcaacgtc gctaactgca
    15481 caaacctccc ctttaaacag cgcattacat acgctgcttt tccccgcgcc agtttttccc
    15541 atgacaccaa tcacgggttc atagtcgatg gcttcactga ttttgttcaa cagcttttcg
    15601 cgcagtggct gagggaaaat atcaagaata tccctgatgt cgggtatgtg cggcttgcta
    15661 tcaccttttt gctcgttgct accattacgt tctggaaata tattgctcca cattcccaaa
    15721 atcccgcctt cccttctggt gacgccactg gcatcagtaa tcatccggcc ttcgcccttt
    15781 accgggtcat ccatacgatg tctccttttt aatttgaaat gaacgttgtc tgttactctc
    15841 tgaaggtctg aattacgtca cttatgaaac gaaagtgagg gagggctgaa ataaactctt
    15901 cactggcagt cagagcgtca agttcaggct ttctgcgaaa agaggtatcc agaacggtta
    15961 cggcgttaac gtcttccatc cgaaacacct gtaagtttct ggtgtgggta gcgacaagaa
    16021 accagttttt ggactgaaat atcagacggc ggggagctac tgcttcgtag cttttaccgt
    16081 taactaataa tgtaacggcg cggtagtcac ggatagcttc cgccagttcc agaaaacttg
    16141 tggtttgtat aactggagat ggcagccctc catgccctat aaggcacggc gagcttccgg
    16201 agttactggt aatcagtcga attaacctgc gattctgagc cggaataatc cctgaaatcc
    16261 cggagttacg gataaacgca aaggtgttgg gtgagtgctc ccggcccgtt cggcgggtaa
    16321 gtcgatagct tccgctggaa tgctcaagat ccagatggat caggcgttgc tgaaaatccc
    16381 gctgtagggt gcgttctgag acaccaaatt catcagctaa cgctttaagc tccagagatt
    16441 cgccagccag taagcgagtg ataatgatcg ataacctgac cgacagacgg tcataatgat
    16501 cgttttgtgt tttcatggtg gggacagtcc tgttctccca aaatatcatg gtattgatac
    16561 gggagattat gccgtacagg tacgacacat tatgtcgtac caaaggggag tggatagaaa
    16621 aaacggcgaa gaagcgaagc gttgattaat tcattgaaat aaaatgaatt aataatgatt
    16681 ttgggtaaaa gcgacgtcaa aatacgacac aacctgtcgt attttgtgtt agccgggaaa
    16741 ggatatctgc tacttactaa atgatttagt gctgaaaata tttataagcc aatcaataga
    16801 caagcattac ggagtaacgc ccaggctatg ttaacgagcc cgctgtttta ttggctcaaa
    16861 aaacatatcc catagatgat cagccatacc ttcgttagtg agtaaggtat cccttgagta
    16921 gccatggctg aagacgccca ttgatgggcg aaaaccaagg ttctggccgt tattgtcaat
    16981 ggttaagatt tcattgtaag cattgccaat cacgccatcc tggctataac caatatcgcc
    17041 tatccccata tttcgcccgc tattaagcca gattgcacat tgacctactt tccttccccc
    17101 aatgtagatc gcacaagtga aggcatttgc gtcacgctta ttaaaatcga cctctaaccc
    17161 ggtatggcgt tttttgagtt cagtcagcga gccttcaaaa taacgggcga catattcaaa
    17221 accgtcacgg caggcacgat ccttatcaag atcggtaaaa cgttttggca gtgaaaggtt
    17281 gctcgagcgt ggtgtaaaaa cggtatccgt tgcctgaagt attggattag ccggagctgg
    17341 ggcgtgcaca atctgttgcg gctttttcgc ttccaaattg gcaatagcgc gggaaacggc
    17401 atcgacaacc tgaacatagc cttcatcatg gctggcaaat ttctcaattg gcttaccgtc
    17461 aacagttgcc gccagaatgc ggccaaacgg taacatcttc cacgcacact ctcttaagat
    17521 cacaggaatg accaccgcct cgccccgttt atgtcgctcc agagcgttgg tcatttctac
    17581 ctgatagcaa tagtcagaag cgatgaaatc actgctaatc aacaacagga tgatatcagc
    17641 ctgagaaaag taatgatcta tttggttctc aaactcttgt cctggaatga tgcgacgatc
    17701 atgccatgtc gtgatcttac ccatgcgctt caggggggaa agatgcttct caagctcatt
    17761 gcgcaatgct tcgtctacat gagaatagga aaaaacgaga gagatcatag tgcgtaagat
    17821 gctccgatag gaaagtggaa aataccgtta ttttgcatct acaagtgtaa aagtgtaatc
    17881 ccccttgcca caaagtattg atgcggaaat cagtccttca tcttggtatc actctgacgt
    17941 cagggtatga tctctggcaa tttccccgat tttacacaac ctggacatcg aaccaggcat
    18001 aaatgatttt ttgaggtttt gatggacaac gctgaacagc aatttctgaa cgatctggat
    18061 actaagttct ggaaagccgc agacaagcta cgcgccaata tggacgcagc caactacaag
    18121 catgtggtac ttgggcttat cttcctgaag tatgtttcag atgcctttga cgcccgtcag
    18181 aaggaactga aaggactgtt tgaagacaag tccaacccgg acaacattta cgcgctgtct
    18241 cgtgacgatt acgacacaga agaggcatac cagcaggcga tcgcagaaga gctggaagtt
    18301 caggattact acactgagaa aaacatcttc tgggtaccca aactggctcg ctgggaaacg
    18361 cttaaaacca atgctgctct gcctgtaggg acggttctgg ggaaagacga tagcggcaaa
    18421 gatatcgtca tgacgtcagt atcgaagctg attgatatcg ctctggatac tatcgaaaac
    18481 agcaacccga aactgaaaaa catccttaac cgtatcgggc actatcagct tggtaacgac
    18541 ctgctaatta gcctgattaa cgttttctcc gataccagtt tcagcgaacc caaatacaat
    18601 ggcgtgaagc tcaatttgaa gagcaaagat attctcggcc acgtttacga atacttcctt
    18661 ggtcagtttg cactggcgga aggcaaacag ggcggccagt attacacgcc gaaaagcatc
    18721 gttacgctga ttgttgaaat gctccagccc tacaatggcc gcgtgtatga cccagcgatg
    18781 gggtctggtg ggttcttcgt ttccagcgat cgctttatcg aagcgcacgc agatgaaaag
    18841 cattacaacg ccgctgaaca aaaacgcaat atctccgttt atgggcagga gtccaaccct
    18901 actacctggc gtctggctgc gatgaacatg gtgattcgcg gtattgattt taacttcggt
    18961 aaacagaacg ccgatacgtt cctgaacgat cagcacccgg atttacgtgc cgattttgtt
    19021 atggcgaatc cgcccttcaa tatgaaagag tggtggaatg cgaagctaga agatgacgtg
    19081 cgttggcagt acggtacacc accgcagggt aacgccaact ttgcctggat gcagcacatg
    19141 atccaccacc tggcaccgaa aggttcgatg gcgttgttgt tggcaaatgg ttcgatgagc
    19201 tctaacacca atagtgaagg ggaaattcgt cgggctatta ttgaggcgga tctggtggaa
    19261 tgtatggtgg ctttaccggg gcagttgttt accaataccc aaatcccggc ctgtatctgg
    19321 ctgctgacca aagataaatc tggcggtaac ggtaaggcgc accgtaaagg cgaagtgttg
    19381 ttcatcgacg ctcgccagat tggttttatg aaagaccgcg tactgcgtga tttctcaacg
    19441 gatgatatca aaaagattgc cgatactttc cacgcctggc agacggataa gaattatgaa
    19501 gatgaggctg gtttttgttt tgctgccaca ctggacgata taaagaaaaa cgattttatc
    19561 ttgaccccgg ggcgctacat tagtgaggtt aatcaggacg acgacaatga gcaatttgcc
    19621 gagaagatgg cgcgattgac cgcacaactt aaagaccagt tggaagaacg cgaaaaactg
    19681 gaagtacaga ttaaggtaaa tctggcggag atgggttatg aattctgatt ggaaaatagt
    19741 taaacttgga gatgtctgca ctatccgacg cggttcttct cctagaccaa ttgttgatta
    19801 tatggcccct aagggaatgc catgggttaa aatatctgat gctactgaga gcaagtctag
    19861 gtatatatct aaaacgaaag aatttataaa gccagagggt attagtcgta gtgttattgt
    19921 gaacaaaggc gatttaattc tttccaatag tggaacggct ggattaccaa agtttatgga
    19981 gataacagca tgtatccatg atggatggca agtatttact gagctagttg atattgataa
    20041 agagtttctt tattacgtat tgttaaatat aaggagcaag cttcttcata atgcttatga
    20101 cagcactatg aaaaacctga ccttagatat ggtgcgtgat gcacaggtta ggctcccaca
    20161 aattaaagaa caaaagagaa tcgcaggtgt tttgtcatca atagatggaa tgattgagtg
    20221 taatcaaaac gtcaatcaaa ccctcgaaca aatggcacag gccctgttca aaagctggtt
    20281 tattgatttt gaaccggtaa aagccaaaat cgcagtgctg gaagcaggtg gttcgcagga
    20341 agatgcaaca cttgctgcca tgacggcaat ttctggaaag gatgctgatt ctttgactat
    20401 ttttaagcga gagtatcctg aaaaatatgc tgaactcagg gctacggcag agttgttccc
    20461 gccgactatg caggaaagtg aattagggga cattccggct ggatggagtt cgcaacgaat
    20521 gtcaaatata gcaacccttc attacggaaa agcattaaaa aaaacagaac gtattgatgg
    20581 gccatatcct gtctatggtt cgggtgggat tacaggttct cataacgctt accttgttga
    20641 agggcctggt attatagttg gcagaaaagg ctcaattggt acaatttatt gggaagatgg
    20701 aaaattccat ccaattgata ctgtctatta cgtagccact aaagaagaaa ttccattaag
    20761 ttatttatat tatttaatga aaacattgaa tctgcccagc atgaacaccg atgctgctgt
    20821 tcctgggtta aatagagata atgtttatcg tcttgaagta ttgaaaccaa ctcaatctat
    20881 cttgaataga ttcgacaatc acatagcaac tatcagaaac agtattcaaa aaaataataa
    20941 cacaatagta tccttagtgg gtttacgcga caccttactc cccaaacttc tctccggtga
    21001 aatccccttg ccggaagccg aggaattagc aaaggaagcg gactatgttt aaagtcgatc
    21061 agcccagtaa tagcctgcaa ccgctccaag aaaccacctt cactaacgcg ggtttcaccg
    21121 aacgctacca cttacaggaa tggttggcga agcatccgca ggcattgacc cgcagcagcg
    21181 atgatgaatt acttattatt cagaaagaat ttgccgggtt tgacgacacc aaagagcggc
    21241 ttgatctgct ggcaatcgac aaagacggta atctggtgat catcgagaac aagctcgatg
    21301 attccgggcg tgatgtcgtg tggcaggcgc tgaagtacgc gggctactgc gcgaacctgc
    21361 gcaaagaaca tatcgttgag atcttccagc gttacctcga tcagaacgat ccagaagaaa
    21421 cacgcccagc ctctgatgtg ctcgcagaat tcctcgaaca tgaaagcctg gatgaagtgc
    21481 aaattaacca aagccgttcc cagcgcgtga tgatggttgc ggcaaatttc cgcaaggaag
    21541 ttaccaatac tgcgctctgg ctgatgcagt tcaatatccg tgtccagtgc tttaaggtca
    21601 cgccttaccg ttatggaaat gatgtattca tcgatatccg ccaggttatt cccacaccgg
    21661 aagccgaatc ctacatgatc ggtatggcgc agaaggaagc agaagaacaa tcaaccagcg
    21721 gtgaactgaa acagagccaa cgcctgcgca aggccttctg gacgcagatg ctggagaaat
    21781 ttcatgccag cgactgtacg ctgtataaca acatcagccc gtctatggat cactggttat
    21841 gtgcaggctc tggcataagt ggtatgccgt atagcctaat attcagcaag aaggaaatcc
    21901 gcgtagaatt atggatttcg cgagcatcgg cagacatcaa caccatggcg ttgaactggc
    21961 tggaacaaca ccgaacatct gtcgaagagg tattcggcaa cccactggag tggctatcgc
    22021 tacctggtaa aaaagcctgc cgtattcaat atcgccaaaa aatagatggc ttccagcagg
    22081 aaaattggcc gcaaatgatc gactggctgg tggaacatat gacccgtttt gagaatgcgc
    22141 tttcgccgta tctgccacag cttaaccagt tgatcaagca gacctttaac gggcatgatg
    22201 aagctgaaaa tacaacagaa gaagccgaac acgtataaca caggacgtta ccgatgctta
    22261 ccgaagacga cttagaacag ctttgcctgg gctggtttgc cgaacaaggc tgggaaattt
    22321 atcacgggcc cgatattgcc cccgatggca gtaacccgca acgcgccagc tatcatgatg
    22381 tattcctgac acccgtgttg aacgcctgcc tgcaaaaaat caacccgcac cttcccactg
    22441 acgtcttcga acaggttgta tcccgtatta ctcgtgctga aagcccggat ctgattgcca
    22501 gtaacaaagc cttccatcac tggctgctgg aaggtgtacc ggttcagtac cgccgcgacg
    22561 aagaacttat ccacgatcac gctctgctgg tggattttaa ccagcagcaa aacaaccgtt
    22621 ttatggtggt caatcagatg accatcagcg gaacgaaaca accccgccgc ccggatatca
    22681 tctgctttat taatggactg ccgctggcgg ttgtggaact gaaaagcccc atcgatgaca
    22741 aagtagatat ctgggatgcg taccaccagt tacagaccta taaagatgaa attcgcgatc
    22801 tttttatcag caatgaagcg ctgattgtca gcgatggtta tctggcgcgt gtggggtccc
    22861 tgaccgccac ggaagaacgt tttatgccgt ggaagaccat cagcaatgaa aatgacaagc
    22921 cgttgctgga atggcaactg gaaactatgg tacggggttt cttcaaccgg gaattgctgc
    22981 tcgactatat ccgctatttc gttctgtttg aaaataacga tgaaaggcta attaagaaaa
    23041 tagctggtta tcaccagttc cacgcggtgc gtgaggctgt gcaggcgacg atcgttgctt
    23101 cgaccggtaa gcaactggta ctgcacagta atattcagcc aggcagtaag aaagcaggcg
    23161 tggtctggca tacgcagggt tccggtaaaa gcatctccat gtgttgctat gtcggcaaac
    23221 tgttgcaaca gccggagatg ggcaacccga cgattgttgt tgtgaccgat cgtaacgatc
    23281 tggacggcca gctttatggt acattctgcc aggcacagga gctgctcaag caaatgccgg
    23341 tacaggcaga tgaccgtgaa acattgcgtg atctgcttgc aactcgcgat gctggcggca
    23401 ttatctttac caccgtgcag aaattctccc cggaagaagg ggaaaacgcc catccgattc
    23461 tcaatggacg cagcaatatc gttgttattt ccgatgaagc gcaccgcagc cagtacggca
    23521 tgaaagccaa cctggatcgt aaaaccggcg tctataaata cggttacgct aaacatatgc
    23581 gcgacgcgct acctaacgcc tcgtttctgg gatttaccgg tacaccggtt tcggcagagg
    23641 atcgcgatac tcgtgctgta tttggcgatt acgtctcaat ttatgatatt caggacgctg
    23701 tggacgatgg cgcaacggtg cctattcact atgaatcccg gctggcaaaa ctcgatctca
    23761 atcatgaaga gctggcggcg ctgtccgatc aggttgaaga actgattgag gacgatgagc
    23821 tggatcagag agaaaaaacc aaaggtgaat ggagccgtct ggagaaactg gttggtgcta
    23881 aaccacgtct tcagcagatc gctgccgatc tgatttctca ctttgagaag cgcaataccg
    23941 taatggacgg caaggcgatg attgtcgcca tgagccgtga aatctgtgtc catctttacg
    24001 atgaaattat tgcgttgcgt ccggagtggc acgatgacga tcctgaaaaa gggcaaatca
    24061 aaatcgtgat gaccggtaca gcatctgata aggcaatgct gcaaccgcat atctacaaca
    24121 aacggactaa aaaacggctg gagtcccgct tcaagaatgt gaacgacccg ctgaagctgg
    24181 tgatagtgcg cgatatgtgg ctgaccggct ttgatgcccc ttgctgtcac actatgtata
    24241 tcgacaaacc gatgcgcggc cataacctga tgcaggctat tgcccgtgtc aaccgggtat
    24301 tcaaagacaa gcccggcggg ctggtggtgg attatatcgg tatcgccaat gagctgaaac
    24361 tggcgctgaa aacttatacc gatgcaaacg gtaaaggcga tgcgaccgtc aacgctgaag
    24421 aagcactggc aatcatgctg gaaaagctgg acatcatcca cggtatgttt gccaaaacgg
    24481 caacgtcagc gggttttgat tacaccggct ttgaagctca tgcgcagaag ttgctagtac
    24541 cagttgcaaa ctatgtgctc ggccttgaag acggtaagaa gcgttttctc gataacgtac
    24601 tggcaattac taaagcctgg tcgctttgtt gtacgctgga tgcggcgcag ccttataagc
    24661 tggagattgc tttcttgtct gcggtgaaag ccgctatcag taaatttacc agtgtggaca
    24721 aaaaaataag ccaggcggag aagaactccg cgctcaaaca gattctggat aatgccgtta
    24781 ttgctaacgg cgtggatgat attttcacgc tggctggtct ggataaaccg aatattggct
    24841 tgctttcaga tgaattcttg caggaagtcc gcgatatgcc atatcgaaat ctggcggtag
    24901 agctgctgga aaaactgatt aatgacggta tccagtcccg tacaaaaaac aatgtagtgc
    24961 aggagaggaa atattcagaa cgtcttcagg ctgtattgat taaatacaac aaccgggcta
    25021 ttgagacggc gcaggtgatt gaagagctga tccagatggc gaaggatttt cagtctgcaa
    25081 tggagcgtga tgatgcgctt ggcctgaatc ctgacgaaat cgctttctac gacgcactgg
    25141 cagaaaacga aagtgccgtc cgagagttag gggatgaggt gcttaagaaa ctggcgatag
    25201 aagtcacctt gaagttgagg caatccacta ccgttgactg gcaggtgcgc gagagcgtgc
    25261 gtgcgagatt acgtatcctg gttcgccaga ctctgcgccg ttataagtat ccgcccgata
    25321 aaacaccggg gacggtggag ttaatattaa aacaagcgga agttatttcg aatcactgga
    25381 cgatgtgaat ttcatgttga cagcttcttg ctgtcaacat cgttacttat tttttgtgat
    25441 aatatgaata cagcacaact acctaaaaaa gcaaatattt ataaagcaat gttttatttt
    25501 ttcctcaaca aggaaaaatt tattacgctt ggtgctatca ataagatggc ttttgaactt
    25561 gagaaggatg aatataagag aaaacttgag gcggggaaat acagtctaaa tccagatgat
    25621 cacaaacact ccctcgaaga gtattgtgat gacttaagaa agtccattct tgcgtcagtg
    25681 ctaataatgt tgggtgtgct ttttatagta attgctattg gattttactc agggaaattt
    25741 cggattatct atccattaga atggaaagac gccgttagcg ccaccggaat atttattcta
    25801 tcatggtcaa cattatttca gctaggctgg aggaatgcat catggaaagg ctgtcgcctt
    25861 gatgaactgg ttggattagc aatatttaga catgttttta ttttcggctt gttccttagc
    25921 ttattatgct tcgcaatata atacaggatt ttaaaaatga caaataagat taggtcgtat
    25981 ctttataaag atacatagca ttgaggaaag atgctaacaa agaaaaaaca ttaataattg
    26041 aagaatccta taaaaccgtg aacaactcca aattttatta aaggagagat aatttatcag
    26101 ccaatactta tttaagaaca aaaaatcatt ttcggcacat tgaagcagca gaacgacaaa
    26161 tttaactaat aaaactggaa tgacatctat gaattagtta tataaaccca catttaacca
    26221 tttattaatg acatgcattt tatctaatat aagcacatga taagcagaca aggtatataa
    26281 actaaaaaaa agacaatatt tctttccaga atagtgagct tagccccttg cttaaaaaac
    26341 aaaacaaatt tcttcaacct aagaaaaaca atgaatttat tatttaaccc catcttattt
    26401 acaaaagttg aaattcttat atagctatac aacgcataaa tacagcaagt acagtaaagg
    26461 ccgacagatg gaaccgttca ataaacgacc acccgcgaaa taaagacaga atagaaataa
    26521 aaataaagaa agacaaccat ttcacattac gcctaaccat ataccctcca cataataaag
    26581 ccgttatcaa taactaactt aataatctaa agaaaaaaat caatctattt cagattccct
    26641 cgcctctgaa cacctctttc ctgtgccatt ccacaaactg ttcgcccgga taattttctt
    26701 taaccggtgg caacgcaatc gcttttcccg caaaatccca gaacagtcgc tgtaccactc
    26761 caccgccatt taccgcaccg gaaaccacca cacgcatatt ttcatccagg ccaatcgaac
    26821 cgcgatcaaa cgctttatga tggattgcgc acaatgccag gccattaggt acttcacaag
    26881 ggccatggtg ctgcttccat ctgatatgag cagcttccag agcaattggc gcattgtcgt
    26941 ggcgcatgtt gaagccgcag atcgcacact gataattgta agcacgtagc accatctggc
    27001 ggaacttcgg atcgcgttga cggagagagg tgcgaatatc aaaacccatc tcgtcagcta
    27061 tctcttcctg aatactggcc gggaaatgcg cctccaggag ctgctgcgcc agcgtatcaa
    27121 ttaacctgtg attttttgcc accaacgcaa aattatcggc atcaaatcca ccagcgacat
    27181 tatattcaat gagttcacgt cggggcggct ggcggctacc actggttgag cacagttgtg
    27241 cgttttgtag ttcccagaag ccatcgccct tcagacgcca gaacggcatg tccgggcgct
    27301 ggtcacggcg ctgcggtcca taacgttcca gaagatccaa caggggctcg tagatttcag
    27361 aaccatagtt aaacaggcga ccatgaccct gccgatagtg tgagaggacg tgcagcagca
    27421 ataatggctt atgtggcgca cgttgttcgc ctttgcgcca gatggtgata tttgatatcg
    27481 cttgctttag ggctttgtca gaggtcattg cgttgaagaa tccatgagca catatttgcg
    27541 atgatgctcg caactatatg tacattcaag gcattattgc agacatcctt tttgcctgac
    27601 tgctctgtac ctggcagatg aaattctgat aacaacggta aaaaatacag cgaccaacca
    27661 gtcctaagcg ggctggttgg tcttttaaat ttaaacatca gccgaggaat aacggcattc
    27721 aactcactcg ccattacccc gattgatctt cgcatagtca aacggcgtaa tcgctctctc
    27781 ccgattggca tccagaaaat cagcccacca ctgcaacatc agcttgcgct catccaggtg
    27841 ttccgcttta tggatatacg ccgccctcac cgagttgcgt tccatatggc tcatctgcct
    27901 ttccaccgca tccttcgacc ataaccctga ctcaatcaat gagctacagg ccattgtgcg
    27961 gaagccgtgg ccgcaaactt caaccttagt gtcataaccc atcacccgta gcgcactgtt
    28021 taccgtgttc tcgctcatgg gcttacgcgg atcgtgatcg ccaataaaaa tcagctcatg
    28081 ttcgccacag aactgtttta tctgcttcag gatcgccagc gcctgctttg aaagcggcac
    28141 cagatgcggt gtacgcatct ttgaacctcg ctgagaatgt ttcacaccgg gaataggctc
    28201 tcgctcaggc gggatagtcc acattgacgt ttcaaaatcg atttctgacc agcgtgcaaa
    28261 acgcagctca ctggatcgaa taaaaataag cagagtaagt tcggttgccc agcgggttag
    28321 tggcctgccg gtgtattcat cgattttctg gagtaattcc gggatacgct ttaactccag
    28381 cgccggacga tgctgtcgat tactggaagc aaccgccccc gccatctctt gtgccgggtt
    28441 ataatcaatt aatccacttt gcactgcata acgcatgatg gccgtggtgc gctgttgaag
    28501 gcgggaagcc acttcaagac gtccggattt ctccacggct ttaataggaa ttaacagatc
    28561 gcgggtgccc agttcagcaa tattgcgagt accaattgcc gggaagatat tgtcctccag
    28621 gctctttttt acacgattag catgatcttc cgaccatttt tggttggtta cgaaccactc
    28681 tctggcaacc ttctcgaaag taataatctc tttcgcctgc tcttctttca cagcacgctt
    28741 atgctcacga ggatcgatac cagcagctac cagctttctg gcctcttccc ttttcttccg
    28801 cgcatccacc agtgaaacat ccgggtaaac gccgaacgcc atcaaatgtt gtttaccgcc
    28861 aaaacggaaa cgaaatcgcc aatactttga accgccaggt tttacaagta aggacatgcc
    28921 gtcgccgtca acaagtgaat actctttctc ctctggtttc gcagaacgaa ctttagtatc
    28981 cgttagggcc atagtggtct tccttccatt ggtatctcgt gagtatacaa gcaatatcga
    29041 atcggcaaat atactcatta gtatactcac aagcctgttg atgtgggttg agtcaggttg
    29101 acttcagttg aggagaaaag ctggaaaagc cttgtgtggc gcggatttca ggcataaaaa
    29161 aagacctcag ttgaggtcta tttacatact tttggtgcga aggccggact cgtatacaca
    29221 tttaacatat tgtaaaaatt gaaatttaat tataattaat tcaattatgc ccctttttgt
    29281 gcccccacca ttctctgttc acattttgat ttttgttgat tatttatcca aaaatcatca
    29341 gagaaatagt caatctagtt aggttccgtt aaacggatcc aaaccgggac ttgactctac
    29401 aaaaaatact tttttttcgc aaaaccatgc acactattca ccctgtcatt tttaacttat
    29461 atatcaattg tttatgtgtt aacagctagg tgaagagtga acagctatct ctacatttgt
    29521 gcgtttccaa taagaagccc ggcatcagcc gggtcagtta atcgttattt taatattggc
    29581 tcatcacact tgggcagcca gtctgcatta ctctcctctt tcaaagtgag gttagtttgc
    29641 attccctgat tagttcgccg tttctcataa tgtagcccgt actccttcag catgactggc
    29701 aaccctttgc caaacatcgt aagactcagg gtgtttttat agccattagc ctccatgtat
    29761 acgaggtagg catggtaaag gtatgtacgt ggctggcgtg ggacaatatt ggcgttcccc
    29821 ataaacatac cgttggtgtc aggtaatgcc tcaagatagc cacaaaaatc aaatgctgga
    29881 tcagcatcac gcttgatggt gagagcctca tctgaattct gctgcgactg aagcaatgtt
    29941 ctggcgctca tcggatcgct gaaacgctgc atgagctggc gaacaatcac agccagttct
    30001 cgtgcaattt tatctttcag ctgcggatcg cgttcttccg gtgctatctg ctcaggaaaa
    30061 tgcagaatca ctctccggcg ggacacacca ccactacgat cagtgaagcg cataggatta
    30121 ttatttacgg ccaggataac cgccggaata tgtgttgaat aagcattctg atatttgggg
    30181 tccaccgata ccgcatcgcc accagttata gccttaagcc cggcgccgtc accgctccac
    30241 ttctcctgat caggcaggcg aataagagaa aaacctatca gagccgcacg ttctcgcggg
    30301 gactccagcg tttcaatggt cgcagaggtg gcgttatcct ctccagcaag catggtcgca
    30361 atttcagcca gaatactttt gccactacca ccagggccag tcacttccag aaagagctgc
    30421 cagtcatagc ggttcgccag caccataaat aacgccgcaa gaataatgtc gcgttttgct
    30481 ggcttgtgtc cggcggcccg gtccagccaa cgccagaatg ccggggcatg agtttcaagc
    30541 gtttccccct ttaccggtgg cgtgaaatcg acttcacaca cggtacgcag ccagttctct
    30601 ttacagtgcg gactaaaaag ccctgttcgc gtatcgagga cgccattacg aaaaccaatc
    30661 agatgacgtg ccggattttg ctgctgcgga acaattaact ttaatgtttc caccagtgag
    30721 gcaatttttc ctgaggaaaa cggtgcaccg aggcgctgga aaagggctgc gacatctcgg
    30781 gcaaaatctg actgagaaat cactttccac gctccagact cgtagtggga taaaagctgc
    30841 ccgttcggat caaccgccag tgcatccctg taatgctcac gaaccctctg agctttttcg
    30901 ctgacactca tcgctgtgaa ttctgcttca ctcattgtgt cgaaaggact ggcgttatgt
    30961 ggcttcaaag cctcaagaat tgccttccgg gtggattcct ctccgtattg ggtaagcgca
    31021 tcattccagt caccaaaaac cggtggcagc acaatgtcac actgacacgc ttttgcagca
    31081 gtttcagccc tgttctggcc tgaaccattc agatcgcggt ccgctgcaat aattaactga
    31141 tatcccggat acttgttatg ggcaactctg gccaaagaaa gaaagtttac cgacgaaaat
    31201 gccaccatga cggcttctcc tgtcagatga tgaatagtaa gcgccgtggc gtaaccttcc
    31261 gcaatccaca tccttcttac agaactcccg cccccttcta tcagatgata ggcctcctta
    31321 acctgacctc ctttaagaaa acacttgcta ccattgccac tgataagctg aatattcacc
    31381 agctccccgt cagcattata gagcgggacg atcaaatcac cggggcggaa catcacccca
    31441 ccggttttat gacctgaagt cagctcatgg caaatatgct ccgggaaacc tttatgtgta
    31501 agataagtat tcccggcaga ttcacgcgag gcttgcagta gtcgcgcggc aagtgctgct
    31561 gccgcctgtt tcccgccatc tgtatctgcg gttgacgtcc agactccaga agaggctgaa
    31621 gacagactat gtgtcagtcc atgtatcctg tctgctgctt cactgatatc cacattcagt
    31681 accttgctga caagcgctaa accgtcccca gccccgcact ggttgcagaa ccatgttcca
    31741 cgaccttcca gatcgtcgaa gcgaaaacga tctttaccgg cgcagaccgg acaaggctga
    31801 tggcaatttt tcagtatgtt cacacccagc gctggcaaaa tccgcgccca gtgaccgcgg
    31861 gcagccttca cggcctgact gactttcatt cctgacatga tgcagttctc cctcagtgta
    31921 aaaccggctt tataatgtga cggacgcata actcatccat tacggctatt ccgagctggg
    31981 agagtgcggg acagggcatt agagtgccgg attccatcag gtctgaaagc agggcgcagg
    32041 cgatttccat gccttttgtt tgcccgtgtt gtcgcaaata aaacccttca agctcacaag
    32101 caatagcggc ttcaatttca tccagagtga gctgcgggtg gcggttttgc tgatggcaga
    32161 cactcagcca ggcgcaggct accgcgcggc gatacaaagc tacccgaagt gaaagggaga
    32221 gagtgcgtga tttcattgca ccacctccat gttcatcagg tcatcctggc aacgctgtac
    32281 cacgccatca agctgctctg tcatcagata aatcagggaa gccagttgct cacattgagc
    32341 accggcaggt ttttcgtggc aatcctgaag aacagccatt ccggtgacaa actcccccac
    32401 gttacgaaga tgcttcaggc ggacaatatc gtcataagag attgcggcgt gattcatcac
    32461 atcacctccc ggacaggcag gcgagcagca aggcaaagca catactcatg gaccagtgaa
    32521 agacgtgcat gatgctcatt gctggcgacg atacgcagca tactgatacg tggtttacgt
    32581 tctgcacgac gaatggcggc aaaaacaaat ataaattgag gatgtgaagg ggcgaggatc
    32641 gtagccataa gggcaacctc caataagtcg cggtaaatgc caccaccgga gttcctacgc
    32701 tcatgggtgg tgacccgaac gggggtagga ataccggcct tattggaaac cggccagccc
    32761 gaaggctgcc ccgcccggac caccattatc tgacagggac taaggtataa gcaccgcagt
    32821 ccaaaaaatg ggtgtgcctg agcaacgacg taaaaaaaga cgcacggcgc gtctggtgtc
    32881 gccaataagt aactcgggtt cctacgcccg gcagccgatt ttgcggcagc ggaaaaacta
    32941 tacctggaaa tgactgcagg atgcaagcca gaaaaatggg gattaatgcg ttcaggcatc
    33001 attatgcatc acatccccgt ccgcgggcac ttatacgctc cgccatccat gcgcttacct
    33061 ctgactgtgc ccaggcaaca tttttaccac caagcgggat ctgcttcggg aaagcatcgc
    33121 ggcttatcag gtcgtatata gttgagcggg acaagccgca taaatgcatc acctcgggaa
    33181 gacgtaaaaa tcgctcatgc tggttcagca caggaaataa tggcgcagcc ggagctggcg
    33241 cagaaacaga aatatcatgc atttatctac cctctttaat tccttcatca gtccggataa
    33301 atccccccgg attcaggtag ttccttatta tgtatatatt tctcgcttac gcacccatta
    33361 tttatttagc gtcacatatt gatttaatgg aatttaaaac tatatcaaac cacataaaat
    33421 ggcatgatag atattatgtc tgcgaactat aaatacaggg acattaatag gaactaactt
    33481 tcatccaggg gttactaccc cataagtcat agaacttaat ggtactgaaa ccgcacccca
    33541 ttcagcaggg atgcacaatt tctacatatg gctttacatt gcaaacctaa cttcaggata
    33601 tactcatgaa ttgcatcaag ctcagaagaa ttagaaccgc ataaaattaa taaccacgca
    33661 ttcttaacct aaaaatatat ccaaataaat tcataaaaga ccgagcagtt ctagttttat
    33721 aaaaatcaga ttgaaatgca acaccaggga aaggggggaa tcatgttatt tcccggcaaa
    33781 gaaaaatcgt ctatttcatc atctgttgaa tactactgaa gagtggtgaa tagttgtgag
    33841 gaacaataat gaaataatca ttcggaataa aaatttcact tttgatgaaa aaaaatcata
    33901 aatcacgcta ccttccgatg aacactcatg aacagtcact gaatactgcc ttgtcacccc
    33961 ttcacctctt aactagctgt attatcaatt tttttatcaa agtgaacagt agtgaatagt
    34021 atttattaaa ggaaagttga agatcagaca tgacacattt ctctggctag ccagaacaag
    34081 gtcattgttc tgtcactgac acaatccccc tcggtagtgc gcttgtatgg gcatcggcac
    34141 aattgacacg acaacaaaac actaccggag cagtcatgac tactgttaac cagatctctg
    34201 atgcaaacat tacatcctcc ctcccaccga aaattcgcga agcggtggaa aaagttaagg
    34261 cagcaaaagc tgtctggcag gaagaacggc gaaaacaaac tgaagctgct gcaatgattg
    34321 aaactatccg taaacgtcag gaagatacaa aaacggaaac gcaggcgctt aatgatgaat
    34381 ggcgaaacct gtttcgtgag aatcagggaa atatgacacc acgcatgaaa aaactgcggg
    34441 cagaaatcgc cctcggacgc gaaacactgg atgagttcga agatttgctg gcagctcagg
    34501 cggcagaaaa tgaattcctg ccctggaaaa ctgcggatgc tgcaaaccac tacatcagcg
    34561 aacataatcg cctgattgaa actcatgcag tgtggctctg gaatgagttt atgaaggaac
    34621 acggccagaa acttattcag atccttggac tgttgaaaat gactctgggc cgaagcgctt
    34681 ctgctgttat cggtgtagtt cataccgtaa acgaccccga aagtgtgctg aagcaattta
    34741 tcagcgagca actcaccact ccggcactgt cctgtaacgt atcttcaacg gatgatatta
    34801 ccctgccggg gatcagcatt tatgcggacg ataaagccat ccaggatgcc agacaatccc
    34861 ccagccctgc agcgcgttca cggatgctta aacagcgtga catggttaaa gggggcgaga
    34921 agaaatgaat gccggaacca ttactcagga agctctcaac gattaccgct cggcgataaa
    34981 aagctggctg acactacgta atacgcaaag tacctaccag cttcgtctgg cggctctgct
    35041 ggatactgaa gaaaaaccgg cagcatatgc cagccagctt gaggagcttc gtgagcgtct
    35101 tgctgtcctc gaatggcaga ttaactgtgc tgcacgggat ggtctttatg ctcaccagat
    35161 tgtgctggag agttgtgtta caagcgcaac ggaaaacttc atgagcgagc atggtgatgc
    35221 actcactgac gcactggctc cttttctttg cgctccatac gggcttgagg cggcaatgaa
    35281 aatattacga accgctgtag cccgacaaac ggaagtgcgt actccggtaa tttcagcagc
    35341 atacaagagc attatcgacg aaaccggatt aacggtggat gcatcaatgt acgctgatgc
    35401 ttcagccagt ttcaccccgg cacaacataa agttttcctg gcccgcctta accgacttaa
    35461 tgaaaaagga gggtactgat atggccctga agtgtcctga atgtgggacg gtcgctcacg
    35521 ccagaaccag cgcctatgaa gctccatcgg ttaaacgctc atggtatcag tgccagaatc
    35581 tggaatgttc ctgcacattt accgctctgg aaagcgtgga tacgataatt atgaagcctt
    35641 ggcgcaatga acaggaatca gacaaagcac aaatgccaga aaaacaacag caaactctca
    35701 accgctatgg ctccgcgtca aagctgtcaa gccgtcagca aattcctgtc tgattagcac
    35761 aatacgccca cgtagtcccg gtccagtacc gggatttttt acgccttttc cctggctggc
    35821 ctgaaggcgt atgagtgcat atctatagcg catgaaaacg catgagtctc atgcgccatt
    35881 tttgacgcga aagcccttgt gtggtggctt ctgagacgat ttacggggtg catgaaaacc
    35941 agtctgttaa gcgaagcggg caggcgggcg gggctgcgcg cgcaaagtgg ttgaaaggta
    36001 tctttaactt ggtggtatac tcttagcctt gttactcatg tgtagagcat ttgatgtaac
    36061 gagccgatta ctatttcaca ctattaagaa ggattagaga tggccagagg cgaattaatg
    36121 aagaaacttt tggctagcta cggtaatgat gatgaatttc gttctgttgc tgaacgaatc
    36181 ataaatgaag aagaaaaaaa gaataataaa gttttggcta atagcctaag aaggacgctt
    36241 gaaaatagcc gagctatagg ttcgaattac tcattaaaag gattggagcg attctctccc
    36301 atgaacgagg ccactaatga ttttgtggaa cgagttgaac caagccattt ccataaagat
    36361 ttagttttat ctttcgataa cgttagaatc cttaaagggt taatagagga gtatcgtttt
    36421 gctgaagcta ttcggaagaa tggtctatca ttacgcacga aacttctttt ttgcggacct
    36481 cccggctgtg gtaagactct aacagcagaa gtgtttgcat ccacgttagg cttgcctatg
    36541 tttatcgtaa aattagataa attgatgtca tcatatctag gcgaaactgc gaccaatata
    36601 cgctcaattt ttgagttcgc caacaataag ccttgcgttc tttttttgga tgagtttgat
    36661 gctattgctc gctctagaga agatagcaat gaacacaatg aacttaaacg agttgtgaac
    36721 agcttgctat tatttattga aaggttatca ccgaaaggtt ttatgattgc agccaccaac
    36781 ttagataaaa gcattgatcc agctgtatgg agacgttttg atgaagtcat atggtttaaa
    36841 aaccctgata aaggaatgat taggagattt ttaaaaagca gattaaaaaa tattaaatat
    36901 aattttaatc cagatgatta tgtttataag ttagaaaaaa tgagtttttc aagtctagag
    36961 agagtgctta atcaagcaat aaaatcaatg attattggaa aacataaagt tttgaatgag
    37021 cgttattttg tcgatgcgat aaatgatgaa catcgaagaa atagtggtgt tgaacaaatg
    37081 cgataatata catggagtct tatatggcag agtatgaaca tttacgactt gttaaaattc
    37141 ctcagcaatt agaaagaaga aaaaaaccgg gatatggttc agctccaaaa cgtaactctc
    37201 aggaaagaac aatacatgga acatctttaa ttcatcaaat tgatagtaca gttgcatatg
    37261 ttcgaagtaa acgtattcct ggaaacattg atccatcatt gatcatgaag gttaaatatg
    37321 atgggtctgc tgccgatgaa gactggttaa ggcttgggtt acaggttcta tctcacgacg
    37381 atgataaaac tttagtatta ttttcttcta acgattcact ggccgattta aaagatagaa
    37441 ttaatgccta ctcagggcct atacccgcaa aacaaaagaa cccatcctac aataatttcg
    37501 taggtaattt aatttcaatt gaaaccatta caagtacaga cagaatagga atccgtttta
    37561 aagaatctgg ttttaatgcc atagatgatt tcgatgaaat ggaaaattat attgtcgata
    37621 ttgaattatg ggattttgga ggaagaatag aaagagaagc gaaaattgaa accattaaaa
    37681 accttattgt taataatggt ggagattttc tagattccta tattggcccc tcaatttcat
    37741 tattgagggc acagtgtaat atatccgcat taaaattagt attagaaata ccagaagtct
    37801 ataccattga cttacccccc atgccagata tggagaccga aaaatacttt aaatttgaat
    37861 tagatgatac accacaaccc attgatgcat atgaagattt accaattata ggtataattg
    37921 acagtggatt aaacaatcat ccttttttag ataacgtaat tgtcggaacc gtgtcttacc
    37981 taaatgatgc tggtcaagac tcttggggac atggcacttc ggttgcggga gttgcagttt
    38041 ttggagactt acgacatcaa ctggctcaga accaactgat tcgttacgct agaatatgct
    38101 cggctaaagt tgtagatgat gaaggacgtt ttccagacga tgtagtaatt cccaaattaa
    38161 tgtcgcaagc aattagacag cttcatcata actttaattg taggatattc aatatttcac
    38221 tcggtgatcc taaagctcct ttcaagggtg atcgaaatgg gcaatgggct tcaattctcg
    38281 atgacttagc tagagatctt gatatattga ttatagtttc cgctggaaat ggttcttctt
    38341 ggggggcaga accagaggat gcaatttctg aatatccaaa ctatttaata gaaaagagaa
    38401 atagatttct tgaacccgca ggtgcagtga atgtattaac ggtaggttcc ttatctcatg
    38461 gagatggatt acctcctgat ctagcagaat atgttggagt catccctacc gcaagaatgg
    38521 atgagccttc gatttttacc agatctgggc cggggatttt aggaatgtgt aaacccgact
    38581 tagtcgatta cggcggaact gcgctttacg acacagccat acataatata aggcctggtg
    38641 gagatattcc tgaatccggc atattaagtt taaacaataa atatttagat agtcttttta
    38701 aagcttcctc tggtacatca gttgcggcgc ctatggtagc taataaggct gcattattgt
    38761 tgaaacaaat accaaatagt acggctaact tactgagagc cttgttagta aacgcctctt
    38821 ctcatccttt agagaatcaa aagcgcatta atttacttga tggaaaatcc aaaaatgaca
    38881 tctatggaaa aggattggta agagtaaaaa atttaatctc atccgaagat aatcgagtag
    38941 tattatactc tcaagatgtt ttacagcctg attatttcgc tgtatatgaa ataccaattc
    39001 cagataactt caagaacacc aaaggtaaaa aaaatatcag tattacactt gcctttgacc
    39061 caccggttag acatacaaga aaagattatc ttggctgtga aatgaacttc aggttaataa
    39121 gaggtaaaac tgccgacgat gtattcaact ttttcaaagt aaaagaggaa ggtgatgcta
    39181 tctctgaaat gcctaaaaaa tataattgtg atctgaagcc aggcgcaaat tctagagatt
    39241 ttgcaacaat acaatcggct tcggtcagtt atgataatgc catagagaat tatggaaata
    39301 cgtaccatct tgttgtacgt tgccaagccg gttgggctga ttttattcag caacaacgtt
    39361 atgctattgt agtcgagctc acgcatactg agaatgtaaa tatttaccag accattcagc
    39421 aaagagttca gccaagagta caaaacagaa atcgaattta aaatactaaa caggcaacca
    39481 tgttgcctgt ttattctatt ttaaattaag tttttttagc aaactcataa ggagtaatca
    39541 tttgtttctt agttgcatct atataatcag accaccattg aaccatcaat cgacgttcat
    39601 ccaagtgttc agacgtatga atatatgccg cacgcacact atttcgttct gaatgactca
    39661 gctgcctttc tatcgcgtca tcgctccata accctgactc acccaatgca ccacgcgcca
    39721 tcgtcctaaa cccatgccca cagacttcgg ttttcgtgtc atagcccatt gcacgtaaag
    39781 cgctgttcac tgtgttttca ctcataacct ttgttgcgtc atgatcccct ggaaaaagta
    39841 gctctttatc accactaatc tgcttaagct gctctaacaa aatcatcgcc tgtcgactaa
    39901 gaggaactat atgctcctct ttcatcttca tgccacgata cgagtaacga acgcctttaa
    39961 tttcttcacg ctttgcaggt atacgccaaa gagatttatc gaagtcgaat tcatcccagc
    40021 gcgcaaaacg tagttcacta gagcgcacaa aggtcagtaa ggaaagctcg accgcgatcc
    40081 gtgtcattat gcggccacga tatgcagcaa gacgagcaag aaactcaggg aagcggctag
    40141 agggtaaagc ggggtaatgt cgcgctttgg ttgtagatag cgcaccggcc atatcactgg
    40201 ctggatttga gtcgatgtaa tcgttttgta cggcgtaacg cattatggcc gtgacgcgct
    40261 gttgcaggcg ctgagcaaca tcgtgtttac cacttgcatc aactttttta atcggggcta
    40321 acaggtgact ggttttgagc tggcgaatgt cggacgaacc gatatgagga aagatataaa
    40381 gctcaagata gcgaagaacg cgcgatcgat ggtcttcact ccatcgcttg ttgctggcgt
    40441 gccattcgcg agcaatagtt tcgaaagtat acgcccctga attctcggct tgagcttctt
    40501 tctgttcggc ttttgggtca atgccctgta ctaatagctt tttagcttca tcgcgcttag
    40561 ctcttgcctg agcaagcgtc accgtaggcc aaacaccaaa agcgaggcga tcctcttttt
    40621 tgtcagaggg ccgtctgtat ttcatgcgcc agtatttaga ccccttggtc gaaacctcga
    40681 gatacaaacc gccgccatcg gccattttgt aggttttgtc ttttggcttt gcagtctcga
    40741 cctgtctggc gttgagcttc atttgggggc acatttctaa tcgaagttaa gatgccccca
    40801 attatgcccc caatgacatc cggatttcaa cggactacct cggacaactc aggaagtaaa
    40861 aatcgctgca agcattgatt ttaaagggtt tgatggactt tctcggatgg tcttggaagt
    40921 actaatggtg ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc
    40981 aactgcgtct accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgaa
    41041 ttatacctgt cacgcgccac catgcaagcg ccagcttgtg cctgcctcgc taagtgctga
    41101 aaatttcagc attgccttca gcgatatgta acctttgtca cactccaggc cccacccagc
    41161 catgctctac acttcccggg caacaccaga gagcgacaaa agcatgtcga tgatcgataa
    41221 cgaataggga tttcacaata tccgttgctt gccggacata aggcgattgg tcgcgtggct
    41281 gccttgggta gcgccgcgca gaataaaggt ttgcaggtag gtcaaaggac ggcgcatggc
    41341 agtggctatt gtgacacctt tattcgctgt aatcagctca acttcaggca aggtgcggcg
    41401 ccgacaatta tgaatcgagg aggctttgcc ggatcgcttc tgccaagcct ttaagatatc
    41461 tcgcaatcgg ctgaatttag tggtgaaaac tttttaaccg tgcctatact ccaggcagga
    41521 aactggagga tatctcatga gcgaacccct gttaattgcc cgcacgccgg acacagaact
    41581 gtttttactg ccgggaatgg ctaaccgtca cgggctgatt actggcgcaa cggggacggg
    41641 taaaactgtc acgctgcaaa aactggcgga gtctctatcg gaaatcggtg ttccggtgtt
    41701 tatggccgat gtgaaaggcg atctgaccgg catcgcgcag gcaggaacgg catcggaaaa
    41761 actactcgca aggcttaaaa atatcggcgt caatgactgg caaccgcatg ctaatccggt
    41821 ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg cgggcgacgg tttcggatct
    41881 ggggccgctg ttgctggcgc ggctgttgaa tctcaacgat gtgcaatctg gcgtgctgaa
    41941 tatcatcttc cgtattgctg acgatcaggg attgttgctg ctcgacttta aagatctgcg
    42001 ggcgattacc cagtacatcg gcgataacgc caaatctttc cagaatcagt acggtaatat
    42061 cagtagcgca tcggttggtg ccatccagcg cggactgttg tcgctggaac agcaaggcgc
    42121 agcacacttc tttggcgagc cgatgctgga tatcaaagac tggatgcgca ccgataccaa
    42181 cggtaaaggc gttatcaata tcctcagcgc cgagaagctt tatcagatgc cgaaactgta
    42241 cgccgccagc ctgctgtgga tgctttcaga gttgtatgaa caattgccgg aagcaggcga
    42301 tctggagaaa ccaaaactgg tgtttttctt cgacgaggca catctgctgt ttaacgatgc
    42361 accgcaggta ctgctggata agattgagca ggtgatacgg cttattcgct caaaaggcgt
    42421 aggcgtctgg ttcgtttcgc aaaacccgtc tgatattccg gataatgtgc tcgggcagct
    42481 cggtaatcgc gttcaacacg ctttgcgtgc ttttacgccc aaagatcaga aagcggtaaa
    42541 agctgcggcg caaaccatgc gggccaatcc ggcatttgat accgaaaagg caatccagga
    42601 actggggacc ggcgaggcgt taatctcgtt tcttgatgtg aaaggaagtc cttcagtggt
    42661 ggaacgggcg atggtgattg cgccttgttc gcggatggga ccggtgacgg aagatgagcg
    42721 taatggcttg attaatcact ctccggtgta tggcaagtac gaggatgagg tggaccggga
    42781 atccgcctat gagatgttgc aaaaaggcgt acaggcgagt attgaacagc aaaataatcc
    42841 tccagcgaaa gggaaagaga tagcggtaga tgacggcatt cttggtggat tgaaggatat
    42901 tttgttcggc actaccggac cacgcggcgg gaagaaagat ggtgtggtgc agacaatggc
    42961 caaaagcgcc gctcgccagg tgacgaatca gattgtacgc gggatgttgg ggagtttgct
    43021 gggggggaga agaaggtaag tcaggcgcga tctgtccccc tcaccctaac cctctcccca
    43081 aaggggcgag gggaccgatc gaactcgtat ttgactttat caacaatgtg aaagggagct
    43141 ttcgctccct tgcttggtta cgattttctc attaacagcc acaggctgat taagaagaag
    43201 ctggcgcttg gcaacagtgc gccgataatc ggcgggatgc cgtaaaccaa cgtcagcggg
    43261 ccgaagatct ggtccagtac gtagaagaca aaaccgaagc tgataccggt gaccacacgc
    43321 acgcccatcg gtacgctacg cagtgggcca aagatgaacg acagcgccat cagcatcatc
    43381 accgccacgg atagcggctg gaagattttg ctccacatat tgagctgata acgtccggca
    43441 tcctgaccgc tcgacttcag atacttcacg tagttgtgca aaccgctgat tgagagtgca
    43501 tccgggtcca gcgccaccac gcccagtttg tctggcgtga ggttggtttt ccaggtgccg
    43561 ctcaccgtct gcgaaccggt aatctgtttc ggattggtca gatcagattc atcaacctgc
    43621 gacagacgcc agactttatg ttccgggtca aacttcgcag tagcggcata gcgtacggat
    43681 tgcagacgac gattttcgtt aaaggcataa atgctgatgc cacctaactc ttcgtcacct
    43741 ttaacccgct caatgtagac gaagttgttg ccatctttcg cccataagcc ttgctgggta
    43801 gagagcaacg agccgccgta catcgcctgc gcacggtagt tacgcgccat ctgctcgccc
    43861 tgcggcgcga cccattcacc aatcgccatc gtcagcaaga ctagcggaat ggcggttttc
    43921 atcaccgaca gcgcaacctg catacgggtg aaaccagaag cctgcatcac caccagttcg
    43981 ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa gcagagctgc catcgggaag
    44041 aagatttgca catctttcgg cacgctcagc aaggtataca ttcctgcgcc taacgcgtcg
    44101 taactcccct gcccggcttt tttcagctga tcgacaaact tgataatgcc cgacagcgac
    44161 accagcatga acagcgtcat catgatggtg gtgaaaatag ttttaccgat atagcggtca
    44221 agtacgccaa aaggttgcat cacaccgctc ctttacgcga aaaactggcg cgcaggcggc
    44281 ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa agccagataa atcaggttaa
    44341 cggtccacat ccacagcgtc gggtccagct tacctttacc gccgttcgat ttcagggagg
    44401 tctggatcag gaagaacaac agatacagca gcatggctgg cagcatcgat agtacgcgcc
    44461 cctgacgcgg gttaaccacg ctcagcggta cgaccataag tgccatcata aacacggtga
    44521 acaccaacgt gatacgccag ttcagttctg cgcgagcacg atcggtgtca gtgttccaca
    44581 atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag cgccaccgcc tggtgaccaa
    44641 tgatcgcctg ataatcctgg aaatccgtaa tgcggaaatc acgtaacagt gcagtgcctt
    44701 cgaagcgcgt tccctggttg agagtgacga cctgggagcc gtcgcgcagc tgggttaaat
    44761 gtccggaatc ggccaccacc acagaaggac gtgcattacc ttttggtcga atttgcgcga
    44821 ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat gaacagcacc gaactgccat
    44881 tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat gccagggttc gctttcgctt
    44941 ctgctaacac ttcatcctga tgacgcgatg accacggtcc cgcccacatc acgttaaccg
    45001 ccgcgacgat tgccgtgaat accgcaagga tcattgccgc tttcaccaga accgctttgc
    45061 tcaggccgca ggcatgcatt accgtaattt cactttcggt atacagtttg cccagcgtca
    45121 tcagcagccc gaggaacagg cttaatggca ggataagctg cgccatttcc ggcacgccca
    45181 acccgagaag ggagagcacc agattcgccg gaatatcgcc gtcaaccgct gcgccgagga
    45241 tcctcactaa cttttgacag aagaagatca aaagcaagat gaagagtatc gccagctggc
    45301 ttttgagcgt ctcccgcacc agatatctta tgattatcac tttaaatacg cccgtaaaaa
    45361 ctcgtctttt gcaggatttt agcttgtttc atggcttaaa cgtcatttat tctcttgagt
    45421 cgtcgaaatc gtcgctaaga taattatact caacggattc acctctcaga ttttgttctg
    45481 acgtgccaat gccgtaataa cgttaagatt aacacgaagt catcgcaaca gcggacatga
    45541 gttacgaaag cttgcaattc tatctgtagc caccgccgtt gtctttaaga ttcaggagcg
    45601 tagtgcatgg agtttagtgt aaaaagcggt agcccggaga aacagcggag tgcctgcatc
    45661 gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg cagaacagct cgataaaatc
    45721 agcgatgggt acatcagcgc cctgctacgt cggggcgaac tggaaggaaa accggggcag
    45781 acattgttgc tgcaccatgt tccgaatgta ctttccgagc gaattctcct tattggttgc
    45841 ggcaaagaac gtgagctgga tgagcgtcag tacaagcagg ttattcagaa aaccattaat
    45901 acgctgaatg atactggctc aatggaagcg gtctgctttc tgactgaact gcacgttaaa
    45961 ggccgtaaca actactggaa agtgcgtcag gctgtcgaga cggcaaaaga gacgctctac
    46021 agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc gtccgctgcg taaaatggtg
    46081 ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc gcgcgatcca gcacggtctg
    46141 gcgattgccg ccgggattaa agcagcaaaa gatctcggca atatgccgcc gaatatctgt
    46201 aacgccgctt acctcgcctc tcaggcgcgc caactggctg acagctacag caagaatgtc
    46261 attacccgcg ttatcggcga acaacagatg aaagagctgg ggatgcattc ctatctggcg
    46321 gtcggtcagg gttcgcagaa cgaatcgctg atgtcggtga ttgagtacaa aggcaacgcg
    46381 tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt taaccttcga ctccggcggt
    46441 atctcgatca agccttcaga aggcatggat gagatgaagt acgatatgtg cggtgcggca
    46501 gcggtttacg gcgtgatgcg gatggtcgcg gagctacaac tgccgattaa cgttatcggc
    46561 gtgctggctg gctgcgaaaa catgcctggc gggcgtgcct atcgtccggg cgatgtgtta
    46621 accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg acgctgaagg ccgcctggta
    46681 ctgtgcgacg tgttaactta cgttgagcgt tttgagccgg aagcggtgat tgacgtggcg
    46741 acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcaca ttactggtct gatggcgaac
    46801 cacaatccgc tggcccatga actgattgcc gcgtctgaac aatccggtga ccgcgcatgg
    46861 cgcttaccgc tgggtgacga gtatcaggaa caactggagt ccaattttgc cgatatggcg
    46921 aacattggcg gtcgtcctgg tggggcgatt accgcaggtt gcttcctgtc acgctttacc
    46981 cgtaagtaca actgggcgca cctggatatc gccggtaccg cctggcgttc tggtaaagcg
    47041 aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt tcctgttaaa ccgcgccggg
    47101 tttaacggcg aagagtaagt ttgcgtttta ccctcaccct aaccctctcc ctaagggaga
    47161 gggaaccgat cgagcgcaat agaaattcat tgctcatctt ttagggtgag gaatgcagca
    47221 atatttaaat ccaccacaag aagccccatt tatgaaaaac gcgacgttct accttctgga
    47281 caatgacacc accgtcgatg gcttaagcgc cgttgagcag ctggtgtgtg aaattgccgc
    47341 agaacgttgg cgcagtggta agcgcgtgct catcgcctgt gaagatgaaa agcaggctta
    47401 ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc tttgttccgc ataatttagc
    47461 gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc tggccgcaaa aacgcagcag
    47521 cagcccgcgc gatatattga ttagtctgcg aacaagcttt gcagattttg ccaccgcttt
    47581 cacagaagtg gtagacttcg ttccttatga agattctctg aaacaactgg cgcgcgaacg
    47641 ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg gcaacctgga aataatggaa
    47701 aagacatata acccacaaga tatcgaacag ccgctttacg agcactggga aaagcagggc
    47761 tactttaagc ctaatggcga tgaaagccag gaaagtttct gcatcatgat cccgccgccg
    47821 aacgtcaccg gcagtttgca tatgggtcac gccttccagc aaaccatcat ggataccatg
    47881 atccgctatc agcgcatgca gggcaaaaac accctgtggc aggtcggtac tgaccacgca
    47941 gggatcgcta cccagatggt cgttgagcgc aagattgccg cagaagaagg taaaacccgt
    48001 cacgactacg gccgcgaagc tttcatcgac aaaatctggg aatggaaagc ggaatccggc
    48061 ggcaccatta cccgtcagat gcgccgtctc ggcaactccg tggactggga gcgtgaacgc
    48121 ttcaccatgg acgaaggcct gtccaatgcg gtgaaagaag ttttcgttcg tctgtataaa
    48181 gaagacctga tttaccgtgg caaacgcctg gtaaactggg atccgaaact gcgcaccgct
    48241 atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt cgatgtggca catccgctat
    48301 ccgctggctg acggcgcgaa aaccgcagat ggtaaagatt atctggtggt tgcgactact
    48361 cgtccggaaa ccctgctggg cgataccggc gtggccgtta acccggaaga tccgcgttac
    48421 aaagatctga ttggcaaata tgtcattctg ccgctggtta accgtcgtat tccgatcgtt
    48481 ggcgacgaac acgccgacat ggaaaaaggc accggctgcg tgaagatcac cccggcgcac
    48541 gactttaacg actatgaagt cggtaaacgt cacgccctgc cgatgatcaa catcctgacc
    48601 tttgacggcg atatccgtga aagtgcccag gtgttcgata ccaaaggtaa cgaatctgac
    48661 gtttattcca gcgagatccc cgcagaattc cagaaactgg agcgttttgc tgcacgtaaa
    48721 gcggtcgttg ctgcggttga cgcactcggc ctgctggaag aaattaaacc acatgacctg
    48781 accgttcctt atggcgaccg tggtggcgtg gtcatcgaac cgatgctgac cgaccagtgg
    48841 tacgtgcgtg ccgatgtcct ggcgaaaccg gcagttgaag cggttgagaa cggcgacatt
    48901 cagttcgtac cgaagcagta cgaaaacatg tacttctcct ggatgcgcga tattcaggac
    48961 tggtgtatct ctcgtcagtt gtggtggggt caccgtatcc cggcatggta tgacgaagcg
    49021 ggtaacgttt atgttggccg caacgaagac gaagtgcgta aagaaaataa cctcggtgct
    49081 gatgttgtcc tgcgtcagga cgaagacgtt ctcgacacct ggttctcttc tgcgctgtgg
    49141 accttctcta cccttggctg gccggaaaat accgacgccc tgcgtcagtt ccacccaacc
    49201 agcgtgatgg tatctggttt cgacatcatc ttcttctgga ttgcccgcat gatcatgatg
    49261 accatgcact tcatcaaaga tgaaaatggc aaaccgcagg tgccgttcca cactgtttac
    49321 atgaccggcc tgattcgtga cgacgaaggc cagaagatgt ccaaatccaa gggtaacgtt
    49381 attgacccac tggatatggt tgacggtatt tcgctgccag aactgctgga aaaacgcacc
    49441 ggcaatatga tgcagccaca gctggcggac aaaatccgta agcgcaccga gaagcagttc
    49501 ccgaacggta ttgaaccgca cggtactgac gcgctgcgct tcaccctggc ggcgctggct
    49561 tctaccggtc gcgatatcaa ctgggatatg aaacgcctgg aaggttaccg taacttctgt
    49621 aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca cagaaggtca ggattgcggc
    49681 ttcaacggcg gcgaaatgac gctgtcgctg gcggatcgct ggattctggc ggagttcaac
    49741 cagaccatca aagcgtaccg cgaagcgctg gacagcttcc gcttcgatat cgccgcaggc
    49801 attctgtatg agttcacctg gaaccagttc tgtgactggt atctggaact gactaagccg
    49861 gtaatgaacg gtggcaccga agccgaactg cgcggtactc gccatacgct ggtgaccgtg
    49921 ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt tcatcaccga aaccatctgg
    49981 cagcgtgtga aagtactttg cggtatcact gccgacacca tcatgctgca gccgttcccg
    50041 cagtacgatg catctcaggt tgatgaagcc gcactggccg acacagagtg gctgaagcag
    50101 gcgatcgttg cggtacgtaa catccgtgca gaaatgaaca tcgcgccggg caaaccgctg
    50161 gagctgctac tgcgtggttg cagcgcggat gcagaacgtc gcgtaaatga aaaccgtggc
    50221 ttcctgcaaa cgctggcgcg tctggaaagc atcaccgtgc tgcctgccga tgacaaaggt
    50281 ccggtttccg ttactaagat catcgacggc gcagagctgc tgatcccgat ggctggcctc
    50341 atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag tggcgaagat cgaaggcgaa
    50401 atcagccgta tcgagaacaa gctggcgaat gaaggctttg tcgcccgcgc accggaagcg
    50461 gtcatcgcga aagagcgtga aaagctggaa ggctatgcag aagcgaaagc gaagctgatt
    50521 gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca gtaatgaaaa cgaaggccgg
    50581 agcatgctcc ggccttttta tctcttacag aaatggtaaa gaaggcataa ttccccgtga
    50641 aatcctggct aagaagcctt tatctggttg ttcttcttta ttgattaaag gaaggtcatc
    50701 caaatcgccc agcaaatacg tattttttgc ctgccagtca atcatccata ttttcagcag
    50761 tggataagcc agaccgcccg taatcccgga tatcaccacc aacgcacaca tgcgataaag
    50821 cataccgtgg tacgttaaag ttgagcgaaa acgaatacgc ccatcattca gtgacaggtt
    50881 gctcataaaa tgatttcgca acgacaccgt taaataactg gtgctaacag caatcccaaa
    50941 ataatagatt aactgcgcga ttatcatttt acgttgcatt tccataaatt gctgtaaatt
    51001 ctcaatatca tcatttgcat acacagaact atcatacgca tttaatattt gatcgatgat
    51061 aatataacca gcaacagcga gaaaaggtaa taaagctaaa aatgcgagaa tggcatattt
    51121 tatacaatac gtagtatcta atttcacctt gaaacgatgt ataccgaaac tggtattgct
    51181 ccagagaaaa ctcattacca ggctatataa agtaccatta aaaataccaa tggaaacaat
    51241 accaactatt gccatcagaa ccacagatat aataacacta cttgaactat tggcatgtag
    51301 catctttgtc gagataaaga aaacagtccc catcccaatg gccattaaaa tcggcaagaa
    51361 aaaggttacc caccagaacc ctttcataga gcaattaaaa ctaaatctta cgccgttgag
    51421 actggtcatc aaggcctgat aacgcagtcc ttttgctgcc ataaatataa gcaaaaccaa
    51481 cagtaacaaa gtcaaaacac agccaacaag tggcatatct gctgacactg tcattaagat
    51541 tgcgaaatag caaaaaacaa aaacaagaca actaacaaaa acattcccac cagtaattcc
    51601 ataagaaaat cgttgtccgt taacttccat attagcatag agataacgct tacatttcat
    51661 taatgcccat ggcaaataga tacctagcgt tataatcgtt aacaacacat tcaccacaca
    51721 aataagaaaa tatctctcac ctgtaccatg aaaaacaaac gaatgggacg gaacatccat
    51781 ttcattaata acttgagcca taaaaattcc ctgaattaaa aaatccattg atataattta
    51841 tatatacaga cagtaaaatg ttctgccacg ttacaatact cttatcagta ataggctggc
    51901 aaaaacttta ttactttata agtcgcttgc gcacgtaaga agagagtggt attaaacaaa
    51961 tgtataatta ctttattttt attgggttaa actatgaata acattgcgcc gcaatcacct
    52021 gtaatgcgtc gcctgacgta gcaggataat cccgccatcg cccgcgtcat tcgtcaggta
    52081 tccgccgaat acggtcttac cgctgataaa ggctacaccg tcgccgatcc gaacctggac
    52141 gagttgtatc aagtgtatag tcagccgggt catgcatatt gggtggttga gtacgatggt
    52201 gaagtggtcg gcggcggtgg gattgcgcca ttaaccggga gtgagtcgga catttgcgaa
    52261 ctgcagaaga tgtattttct tccggctatt cgcggtaaag ggctggcgaa aaaactggcc
    52321 ttaatggcga tggagcaggc acgagagatg ggcttcaaac gctgctatct ggaaacgacc
    52381 gcttttttga aagaagcgat tggtttgtat gaacatttag gttttcaaca tatcgactat
    52441 gcacttggct gcacgggcca tgtagattgt gaagtgcgga tgctacgcga actctaaaaa
    52501 aatgcccgct agtgtgtgct atcgggcatt ttaatcaatt aattagtggc gaaccccgtc
    52561 atcgtcttca tcgacaaaat cttcatcgtc gccgtcttcg ccattgggat cttcaaagta
    52621 agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca gttgttcaac
    52681 ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc agcaaatcac
    52741 gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac cgagtttaaa
    52801 cgcttcaact gctgcttttt ccagggtttc cagatcgtct gcggaaagat ggtgttcgat
    52861 ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa tcaaacgtgt
    52921 ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct cttaatgtcc
    52981 tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg acaaagattt
    53041 atgctttaga cttgcaaatg aataatcatc catataaatt gaattttaat tcattaaggc
    53101 gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg aaattactcg
    53161 atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg aaagccgata
    53221 agaaaagcgg caaagaagaa gccaaactca ctggtaaaaa catcgcgctc atcttcgaaa
    53281 aagactcgac ccgtacccga tgctctttcg aagttgccgc atatgaccag ggtgctcgcg
    53341 ttacttatct cggcccaagc ggcagtcaga ttggtcataa agagtcgatt aaagacactg
    53401 cccgcgtgct gggtcgcatg tatgacggta ttcagtatcg cggctatggt caggagattg
    53461 tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc aatgagtttc
    53521 accccacgca gttgctggcg gatctcctca ccatgcaaga gcatttgccc ggtaaagcgt
    53581 tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc aattcgatgc
    53641 tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag gcatgctggc
    53701 cggaagctgc gctggttacg gaatgccgca ccctggcaca gcaaaatggc gggaatatta
    53761 cgctgacgga agatgtagcc aaaggagttg aaggtgctga ctttatctat accgatgtat
    53821 gggtgtcgat gggggaagca aaagagaaat gggcagaacg gattgcatta ctgcgtgatt
    53881 atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa ttcctccact
    53941 gcctgcccgc gtttcatgac gaccagacga cgcttggtaa gaaaatggcg gaagagtttg
    54001 gcctacatgg cggaatggaa gtgactgatg aggtcttcga atctgccgcc agcattgttt
    54061 ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg acgctcagta
    54121 aataattgat atcgctataa agatgccggg aaatacaaaa acatcccggc atcacattat
    54181 tttatgcagt aatagttcaa ttattccatc catatttttc tattgctata cgtaaatctt
    54241 ccggaggatt ttcatcattc ttgttctaat ttttaattca ttaaccatct tttcttcaaa
    54301 aatcttaacg acataacccg gattgttttt tacaatttca tttgtttttt cttcattacc
    54361 ataaacatat cctaactcat taagatacag ctgttcgccc tcataatctt taagtaattg
    54421 atagaaatac cacttagcaa aatcaggttg cttcatgaaa aaatccttca aaaaaaatat
    54481 catttactaa aatgctgaca ggcacttcat taaatagcaa cttaacatca tcaaataacc
    54541 ttagtattaa gcgtgatttt tcttgattgc ttaagttaaa atcattgaca tatgcaacca
    54601 acttatccca aatcaattcc tgtaatttac ggctattttt atagtgttct ttatcgagat
    54661 tgaaaaataa ggtctcagct attttaattt tagattgatc gcagctaaaa tctttagatt
    54721 gatcgcggct aaaatcaata tgatttatca cggagcatgc atactttatc ttttcagaat
    54781 caccaaaata ttgcattctt ttattaaaac accaatccag tattcttaca tcattaatat
    54841 atgggaattt taaaaaaatc tccagaatcg aatcgatgac cggtgtaagg ttaacttttg
    54901 catcatctaa ggaagcaata agaatatgag acaggttaac tttgatgtca ttaatttcat
    54961 tgtcagtagc tttcttatta ttaagaagat aattatttac tgcgttaagg tcatctattt
    55021 ctttcagtat ccctttttca ccaagcaagt atgaaatcga ttcattacat acataaggca
    55081 ttaatagcag atgttctttc cttattgcta ataatgcatt attcaatggt ttttctaata
    55141 aatccgtaat cgtagaatca actgaatctt tacaactcac ctgcgagtta ttcaagtcct
    55201 gagaattatt taactcctgc gaaatattta atagacgagc tatttccttg ttagcttcgc
    55261 gttggtaaca cgccagaatc ttgttagtaa tcaggtgtaa tgaactctca tcttttcttt
    55321 caaaggactt cccttcataa cgattagtcg aaccatcagg ttgtggaaat ataatgactg
    55381 tatgtccttc acgaatccca ttattaaaag ctgaggcaag tgtaatttct atttccacct
    55441 tagataaatc cgctcgctcc ccttcaggat tattcataac aaacttcatt atctcactac
    55501 catcattatt tttgatataa gatgaggact taatatgagc catcatattt ctacccgctt
    55561 cgttggagcg tttgctaaaa tccacataac ccagggtaaa catcttagcc aaaaactcca
    55621 aaaatgctct gggagaaaga atattgcttc tgctattctg ccctccggca attcgggcaa
    55681 aatcattcat tcccacatac caggtatcta agtcttcatt tttttgttta caattgtcgg
    55741 ctgatgtaat gttttgagaa aaattccaac ctgatatttt gctcatgacg gactccttga
    55801 gttaataaga acgtctaagc attacaaatt ctactcacaa aaaaatatca aaaaacgctc
    55861 ttttcacaaa ctcgccgtaa acctggagtt atttacggcg aaaagtcatc acgccatcag
    55921 catcttcact actgctttgc gaacctgggc tggcgcgccc actgcgcaca gcggtttatg
    55981 cacttccccc ggataaaaca caacaaaatc accttcatta aggataactg ttttctcatc
    56041 aacgccttcc ggcaaaaatg cgatgtcttt atcagctaac cagtcggtat ccggcgtgcc
    56101 tgcaggttgc gtgctgaagg tcatgccttc ctgacctttt aacacaatct gaatgtcgag
    56161 atagcgggca tgatactccg cacggcgagc ttcgtacggc tcggtcatat cttccgagat
    56221 aagataaaac agacgattgc cttcgatatc gtgcttgccc tttggcgttt ctaccgtaac
    56281 gtgtgctttg atatgctcaa tcgcctggcg taactcctgg ggtagccacg gctgaagatt
    56341 atgaatattt ccgatgatca tcttttccac tccttaatta aaacaacgtt tcatttctat
    56401 cgttatacga aaagatgatc aattccgcta gccttaacgc gtgatttatt gtcttatcac
    56461 aacttatcgg caggcgcgtt cccgcctttc gggtcaaggc ccgcatcact ccttgagaat
    56521 agtttttatc gctccccccg ccagtgcagc tgttgcacag agttgttcga gggagtgccc
    56581 ctctcgtgcc ttcgcagaaa gccctgacat cacggtactc ataaaatcag tcacacattg
    56641 tgcgctttgt ggatgccgcc tggcgatata gtcataaatg gccgtttctg cggcgtgata
    56701 atattgaacg gcaatatcac gcgcttgtgg atcatgacta tgaatgcctt caagaaccat
    56761 acagccagcg cagccgccgt tttggctata tctgcgcgcc gcttctttta atacctcagc
    56821 caggcactcc cctactggac gatcatcacg aagaatatcg acaagcggaa tagcttccgt
    56881 accgacatat tcattgagta cacggctaaa taacccagcc ttactgccaa aagccgcgta
    56941 gaggctcggg gggttaatac caagataatc agtaacttca gcaacactga cagcatcgaa
    57001 acctttttgg tgaaaaagca cttttgccgc agagactgcc tgctcaggag cgaaacgtct
    57061 gggacgacct ggaacgcgag attgtttttt agtgaccatt acaaaacttg ttgacagaaa
    57121 gttaaaacag ttttgtaatg catgttacat aataaatcaa ggagtcctta tgggcgcttt
    57181 tacaggtaag acagttctca tcctcggtgg tagccgtgga atcggtgccg ctatcgtacg
    57241 tcgtttcgtc accgatgggg ccaatgtacg attcacctat gcggggtcga aagatgccgc
    57301 tgaacgcctg gcacaagaga ctggagcaac agcagtattc acagatagtg ctgacagaga
    57361 cgctgttatt gatgtcgttc gtaagagcgg cgcattggat atcctcgtgg taaatgcagg
    57421 tattggcgtc tttggcgatg ccctggaatt aaatgccgac gatattgatc gccttttcaa
    57481 aatcaatatt catgctcctt accatgcctc cgttgaagcc gcccggcaga tgcccgaagg
    57541 cgggcgcatc ttaatcatcg gctccgtgaa tggcgatcgt atgcctgttg caggcatggc
    57601 tgcttatgcc gccagcaaat ctgccctgca aggcatggcg cgcgggctgg cccgtgattt
    57661 tggaccgcgt gggatcacca tcaacgtcgt ccagccaggg ccaattgata ccgacgctaa
    57721 tcccgccaac gggccaatgc gcgatatgtt gcatggtttt atggctatca aaagacatgg
    57781 gcaaccggaa gaggtcgctg gtatggtcgc atggttagca gggccagaag ccagctttgt
    57841 taccggcgcg atgcatacca ttgatggcgc gtttggcgca taaccgacta cgctcaatta
    57901 agcccagcca tttcccatga tgtctgggtt ttgtttactc acgtcgtccg ctaaaagcgg
    57961 ctcctggtaa atataaatct tctgttcatg tcgtaaatct tgaggataac caaatggtag
    58021 aaagaaccgc tgttttccct gctggccgac attcactgta tgctgaacat cgttattctg
    58081 cggctattcg ctccggcgat ttgctgtttg tttccgggca agtaggaagt cgagaggacg
    58141 gaacaccaga acccgatttt cagcaacaag tcagactggc atttgataat ttgcatgcga
    58201 ccctggctgc tgcgggatgc acttttgacg atatcattga tgttacgagc tttcataccg
    58261 atccagaaaa acaattcgaa gacatcatga cggtgaaaaa tgaaatattt agcgccccac
    58321 cttatccaaa ctggacggcg gtaggtgtta catggctggc aggctttgat tttgaaatta
    58381 aagtgatagc gcgcatccct gagcagtaag caatagtgtt agccgttcgt tttcacactc
    58441 cgccctataa gtcggatgaa tggaataaaa tgcatatctg attgcgtgaa agtgaaaaag
    58501 gaaaaagcag ggaatgtctg caattattga taccgaagga cagttcccct gcagaatcac
    58561 atcaaataaa aatgcatata ccttgacttt taattcaaat aaaccgtttg cgctgacaaa
    58621 atattgcatc aaatgcttgc accgcttctg acgatgagta taatgccgga caatttgccg
    58681 ggaggatgta tggttcagtg tgttcgacat tttgtcttac cgcgtctgaa aaaagacgct
    58741 ggcctgccgt ttttcttccc gttgatcacc cattcccagc ccctcaatcg aggggctttt
    58801 ttttgcccag gcgtcaggag ataaaagatg gctaatccgc tatatcagaa acatatcatt
    58861 tccataaacg accttagtcg cgatgacctt aatctggtgc tggcgacagc ggcgaaactg
    58921 aaagcaaacc cgcaaccaga gctgttgaag cacaaagtca ttgccagctg tttcttcgaa
    58981 gcctctaccc gtacccgcct ctctttcgaa acatctatgc accgcctggg tgccagcgtg
    59041 gtgggcttct ccgacagcgc caatacatca ctgggtaaaa agggcgaaac gctggccgat
    59101 accatttcgg ttatcagcac ttacgtcgat gcgatagtga tgcgtcatcc gcaggaaggt
    59161 gcggcgcgcc tggccaccga gttttccggc aatgtaccgg tactgaatgc cggtgatggc
    59221 tccaaccaac atccgacgca aaccttgctg gacttattca ctattcagga aacccagggg
    59281 cgtctggaca atctccacgt cgcaatggtt ggtgacctga aatatggccg caccgttcac
    59341 tccctgactc aggcgttagc gaagttcgac ggcaaccgtt tttacttcat cgcgccggac
    59401 gcgctggcaa tgccgcaata cattctggat atgctcgatg aaaaagggat cgcatggagt
    59461 ctgcacagct ctattgaaga agtgatggcg gaagtagaca tcctgtacat gacccgcgtg
    59521 caaaaagagc gtctggaccc gtccgagtac gccaacgtga aagcgcagtt tgttcttcgc
    59581 gccagcgatc tccacaacgc caaagccaat atgaaagtgc tgcatccgct gccgcgtgtt
    59641 gatgagattg cgacggatgt tgataaaacg ccacacgcct ggtacttcca gcaggcaggc
    59701 aacgggattt tcgctcgcca ggcgttactg gcactggttc tgaatcgcga tctggtactg
    59761 taaggggaaa tagagatgac acacgataat aaattgcagg ttgaagctat taaacgcggc
    59821 acggtaattg accatatccc cgcccagatc ggttttaagc tgttgagtct gttcaagctg
    59881 accgaaacgg atcagcgcat caccatcggt ctgaacttgc cttctggcga gatgggccgc
    59941 aaagatctga tcaaaatcga aaataccttt ttgagtgaag atcaggtaga tcaactggca
    60001 ttgtatgcgc cgcaagccac ggttaaccgt atcgacaact atgaagtggt gggtaaatcg
    60061 cgccccagtc tgccagagcg catcgacaat gtgctggtct gcccgaacag caactgtatc
    60121 agccatgccg aaccggtttc ttccagcttt gccgtgcgaa aacgcgccaa tgacatcgcg
    60181 ctcaaatgca aatactgtga aaaagagttt tcccataatg tggtgctggc caattaattg
    60241 cggttggtaa taaaagtctg gctccctata atgagccaga ctttttaccg ctgtaataaa
    60301 ggagaaatca tgagcaaaac tatcgcgacg gaaaatgcac cggcagctat cggtccttac
    60361 gtacagggcg ttgatctggg taatatgatc atcacctccg gccagatccc ggtaaatccg
    60421 aaaacgggcg aagtaccggc agacgtcgct gcacaggcac gtcagtcgct ggataacgta
    60481 aaagcgatcg tcgaagccgc aggcctgaaa gtgggcgaca tcgttaaaac caccgtgttt
    60541 gtgaaagatc tgaacgactt cgcaaccgtt aatgctacct acgaagcctt cttcaccgaa
    60601 cacaatgcca ccttcccggc acgttcttgc gttgaagttg cccgtctgcc gaaagacgtg
    60661 aagattgaga tcgaagcgat cgctgttcgt cgctaatctt gatggaaatc cgggctatca
    60721 tgcccggatt aagtctgatg acaaacgcaa aat
//
LOCUS       DAEVZA010000011        60467 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEVZA010000011 DAEVZA010000000
VERSION     DAEVZA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.011"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..915
                     /locus_tag="LAF92_000743"
     CDS             <1..915
                     /locus_tag="LAF92_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9789797.1"
                     /translation="LDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYMLL
                     NNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSSV
                     LFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKMV
                     RPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFPK
                     SKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRYY
                     RVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            927..1793
                     /gene="ydiB"
                     /locus_tag="LAF92_000744"
     CDS             927..1793
                     /gene="ydiB"
                     /locus_tag="LAF92_000744"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBI9789798.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            1824..2582
                     /gene="aroD"
                     /locus_tag="LAF92_000745"
     CDS             1824..2582
                     /gene="aroD"
                     /locus_tag="LAF92_000745"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBI9789799.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            2726..4321
                     /locus_tag="LAF92_000746"
     CDS             2726..4321
                     /locus_tag="LAF92_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBI9789800.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMNERLFIDAAMGFVLPEAAH"
     gene            4335..5486
                     /locus_tag="LAF92_000747"
     CDS             4335..5486
                     /locus_tag="LAF92_000747"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBI9789801.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(5529..6440)
                     /locus_tag="LAF92_000748"
     CDS             complement(5529..6440)
                     /locus_tag="LAF92_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBI9789802.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            6756..7520
                     /locus_tag="LAF92_000749"
     CDS             6756..7520
                     /locus_tag="LAF92_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBI9789803.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            7540..8478
                     /locus_tag="LAF92_000750"
     CDS             7540..8478
                     /locus_tag="LAF92_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBI9789804.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIIDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            8534..9823
                     /locus_tag="LAF92_000751"
     CDS             8534..9823
                     /locus_tag="LAF92_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI9789805.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SGHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNKPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            9820..10113
                     /locus_tag="LAF92_000752"
     CDS             9820..10113
                     /locus_tag="LAF92_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBI9789806.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            10170..11816
                     /gene="fadK"
                     /locus_tag="LAF92_000753"
     CDS             10170..11816
                     /gene="fadK"
                     /locus_tag="LAF92_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBI9789807.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYNYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(11873..14251)
                     /gene="ppsA"
                     /locus_tag="LAF92_000754"
     CDS             complement(11873..14251)
                     /gene="ppsA"
                     /locus_tag="LAF92_000754"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBI9789808.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            14584..15417
                     /gene="ppsR"
                     /locus_tag="LAF92_000755"
     CDS             14584..15417
                     /gene="ppsR"
                     /locus_tag="LAF92_000755"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBI9789809.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            15574..16620
                     /gene="aroH"
                     /locus_tag="LAF92_000756"
     CDS             15574..16620
                     /gene="aroH"
                     /locus_tag="LAF92_000756"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBI9789810.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQASGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            16752..16943
                     /gene="hemP"
                     /locus_tag="LAF92_000757"
     CDS             16752..16943
                     /gene="hemP"
                     /locus_tag="LAF92_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBI9789811.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(16947..18383)
                     /locus_tag="LAF92_000758"
     CDS             complement(16947..18383)
                     /locus_tag="LAF92_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBI9789812.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(18446..19159)
                     /gene="ydiV"
                     /locus_tag="LAF92_000759"
     CDS             complement(18446..19159)
                     /gene="ydiV"
                     /locus_tag="LAF92_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBI9789813.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(19406..19870)
                     /locus_tag="LAF92_000760"
     CDS             complement(19406..19870)
                     /locus_tag="LAF92_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI9789814.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(19948..20697)
                     /gene="btuD"
                     /locus_tag="LAF92_000761"
     CDS             complement(19948..20697)
                     /gene="btuD"
                     /locus_tag="LAF92_000761"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBI9789815.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(20697..21248)
                     /gene="btuE"
                     /locus_tag="LAF92_000762"
     CDS             complement(20697..21248)
                     /gene="btuE"
                     /locus_tag="LAF92_000762"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBI9789816.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(21311..22291)
                     /gene="btuC"
                     /locus_tag="LAF92_000763"
     CDS             complement(21311..22291)
                     /gene="btuC"
                     /locus_tag="LAF92_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBI9789817.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(22392..22691)
                     /gene="ihfA"
                     /locus_tag="LAF92_000764"
     CDS             complement(22392..22691)
                     /gene="ihfA"
                     /locus_tag="LAF92_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBI9789818.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(22696..25083)
                     /gene="pheT"
                     /locus_tag="LAF92_000765"
     CDS             complement(22696..25083)
                     /gene="pheT"
                     /locus_tag="LAF92_000765"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBI9789819.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVEAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITSEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(25098..26081)
                     /gene="pheS"
                     /locus_tag="LAF92_000766"
     CDS             complement(25098..26081)
                     /gene="pheS"
                     /locus_tag="LAF92_000766"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBI9789820.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(26290..26414)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(26364..26408)
                     /gene="pheM"
                     /locus_tag="LAF92_000767"
     CDS             complement(26364..26408)
                     /gene="pheM"
                     /locus_tag="LAF92_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBI9789821.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(26531..26887)
                     /gene="rplT"
                     /locus_tag="LAF92_000768"
     CDS             complement(26531..26887)
                     /gene="rplT"
                     /locus_tag="LAF92_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBI9789822.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(26940..27137)
                     /gene="rpmI"
                     /locus_tag="LAF92_000769"
     CDS             complement(26940..27137)
                     /gene="rpmI"
                     /locus_tag="LAF92_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBI9789823.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(27234..27776)
                     /gene="infC"
                     /locus_tag="LAF92_000770"
     CDS             complement(27234..27776)
                     /gene="infC"
                     /locus_tag="LAF92_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBI9789824.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(27780..29708)
                     /gene="thrS"
                     /locus_tag="LAF92_000771"
     CDS             complement(27780..29708)
                     /gene="thrS"
                     /locus_tag="LAF92_000771"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBI9789825.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            30229..32127
                     /gene="espL1"
                     /locus_tag="LAF92_000772"
     CDS             30229..32127
                     /gene="espL1"
                     /locus_tag="LAF92_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HBI9789826.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTASCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAC
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHIPLIRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSVYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIKII
                     TAQNSVGTPALFLAMMNGHADNVKIFMQEIQSLVYNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIIDLLKGATAHGTPALYIAMSKGNEDVVLSYISTL
                     GTFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAIDIISQSLSFS
                     ADELKTYL"
     gene            32300..32407
                     /locus_tag="LAF92_000773"
     CDS             32300..32407
                     /locus_tag="LAF92_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789827.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(32460..33218)
                     /locus_tag="LAF92_000774"
     CDS             complement(32460..33218)
                     /locus_tag="LAF92_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBI9789828.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            33505..34434
                     /gene="pfkB"
                     /locus_tag="LAF92_000775"
     CDS             33505..34434
                     /gene="pfkB"
                     /locus_tag="LAF92_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBI9789829.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            34535..34825
                     /gene="ghoS"
                     /locus_tag="LAF92_000776"
     CDS             34535..34825
                     /gene="ghoS"
                     /locus_tag="LAF92_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBI9789830.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINKLNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            34931..35791
                     /locus_tag="LAF92_000777"
     CDS             34931..35791
                     /locus_tag="LAF92_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBI9789831.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(35832..36368)
                     /locus_tag="LAF92_000778"
     CDS             complement(35832..36368)
                     /locus_tag="LAF92_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBI9789832.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            36515..37183
                     /gene="hxpB"
                     /locus_tag="LAF92_000779"
     CDS             36515..37183
                     /gene="hxpB"
                     /locus_tag="LAF92_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBI9789833.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            37346..37936
                     /locus_tag="LAF92_000780"
     CDS             37346..37936
                     /locus_tag="LAF92_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI9789834.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            38069..39460
                     /gene="tcyP"
                     /locus_tag="LAF92_000781"
     CDS             38069..39460
                     /gene="tcyP"
                     /locus_tag="LAF92_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HBI9789835.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(39464..40278)
                     /locus_tag="LAF92_000782"
                     /pseudo
     CDS             complement(39464..40278)
                     /locus_tag="LAF92_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40555..40818)
                     /gene="cedA"
                     /locus_tag="LAF92_000783"
     CDS             complement(40555..40818)
                     /gene="cedA"
                     /locus_tag="LAF92_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBI9789836.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            41001..43262
                     /gene="katE"
                     /locus_tag="LAF92_000784"
     CDS             41001..43262
                     /gene="katE"
                     /locus_tag="LAF92_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBI9789837.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(43309..44067)
                     /gene="chbG"
                     /locus_tag="LAF92_000785"
     CDS             complement(43309..44067)
                     /gene="chbG"
                     /locus_tag="LAF92_000785"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBI9789838.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(44080..45432)
                     /gene="celF"
                     /locus_tag="LAF92_000786"
                     /gene_synonym="chbF"
     CDS             complement(44080..45432)
                     /gene="celF"
                     /locus_tag="LAF92_000786"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBI9789839.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(45537..46379)
                     /gene="chbR"
                     /locus_tag="LAF92_000787"
     CDS             complement(45537..46379)
                     /gene="chbR"
                     /locus_tag="LAF92_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBI9789840.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(46387..46737)
                     /gene="chbA"
                     /locus_tag="LAF92_000788"
     CDS             complement(46387..46737)
                     /gene="chbA"
                     /locus_tag="LAF92_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBI9789841.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(46788..48146)
                     /gene="chbC"
                     /locus_tag="LAF92_000789"
     CDS             complement(46788..48146)
                     /gene="chbC"
                     /locus_tag="LAF92_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBI9789842.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YDSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(48231..48551)
                     /gene="chbB"
                     /locus_tag="LAF92_000790"
     CDS             complement(48231..48551)
                     /gene="chbB"
                     /locus_tag="LAF92_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBI9789843.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(48850..49188)
                     /gene="osmE"
                     /locus_tag="LAF92_000791"
     CDS             complement(48850..49188)
                     /gene="osmE"
                     /locus_tag="LAF92_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBI9789844.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            49390..50217
                     /gene="nadE"
                     /locus_tag="LAF92_000792"
     CDS             49390..50217
                     /gene="nadE"
                     /locus_tag="LAF92_000792"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBI9789845.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            50447..51334
                     /gene="cho"
                     /locus_tag="LAF92_000793"
     CDS             50447..51334
                     /gene="cho"
                     /locus_tag="LAF92_000793"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBI9789846.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(51294..51869)
                     /gene="ves"
                     /locus_tag="LAF92_000794"
     CDS             complement(51294..51869)
                     /gene="ves"
                     /locus_tag="LAF92_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBI9789847.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(52072..52557)
                     /gene="spy"
                     /locus_tag="LAF92_000795"
     CDS             complement(52072..52557)
                     /gene="spy"
                     /locus_tag="LAF92_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBI9789848.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(52887..53855)
                     /gene="astE"
                     /locus_tag="LAF92_000796"
     CDS             complement(52887..53855)
                     /gene="astE"
                     /locus_tag="LAF92_000796"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBI9789849.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(53848..55191)
                     /gene="astB"
                     /locus_tag="LAF92_000797"
     CDS             complement(53848..55191)
                     /gene="astB"
                     /locus_tag="LAF92_000797"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBI9789850.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(55188..56666)
                     /gene="astD"
                     /locus_tag="LAF92_000798"
     CDS             complement(55188..56666)
                     /gene="astD"
                     /locus_tag="LAF92_000798"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBI9789851.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADATQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(56663..57697)
                     /gene="astA"
                     /locus_tag="LAF92_000799"
     CDS             complement(56663..57697)
                     /gene="astA"
                     /locus_tag="LAF92_000799"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBI9789852.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(57694..58914)
                     /gene="astC"
                     /locus_tag="LAF92_000800"
     CDS             complement(57694..58914)
                     /gene="astC"
                     /locus_tag="LAF92_000800"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBI9789853.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            59360..60166
                     /gene="xthA"
                     /locus_tag="LAF92_000801"
     CDS             59360..60166
                     /gene="xthA"
                     /locus_tag="LAF92_000801"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBI9789854.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            60333..>60467
                     /locus_tag="LAF92_000802"
     CDS             60333..>60467
                     /locus_tag="LAF92_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI9789855.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     L"
BASE COUNT        15444 a        15487 c        14434 g        15102 t
ORIGIN      
        1 ctggatacgg gtggctaccc cgcgctcatg gaatgctttc cgaaagcctc tggttcggcg
       61 gtcatactgg ttaaagcgat ggtgtcattc ggacaaatgt tctacccaat gctggtgagc
      121 tatatgttgc tcaataacat ctggtacggc tatgggctga ttattccagg catcctattt
      181 gtactgatca cgctgatgct gttgaaaagc aaattcccca gccagttggt ggacgccagc
      241 gtagctaatg aattaccgca aatgaacagc aaaccgttag tctggctgga aggtgtttca
      301 tcggtactgt tcggtgtagc cgcattctcg accttttatg tgattgtggt gtggatgccc
      361 aaatatgcga tggcttttgc tggtatgtca gaagctgagg cattaaaaac catctcttat
      421 tacagtatgg gctcgttggt ctgtgtcttt atttttgccg cactactgaa aaaaatggtc
      481 cggcccatct gggctaatgt atttaactct gcactggcaa caataacagc agccattatc
      541 tacctgtacc cttctccact ggtgtgtaat gccggcgcct ttgttatcgg tttctcagca
      601 gctggcggca ttttacagct cggtgtttcg gtcatgtcag agtttttccc aaaaagcaaa
      661 gccaaagtca ccagtattta tatgatgatg ggtggactgg ctaactttgt tattccgctg
      721 attaccggtt atctgtcgaa catcggcctt caatatatca ttgttctcga ttttactttc
      781 gcgctgctgg ccctgattac cgcaattatt gtttttatcc gctattaccg cgtttttatt
      841 attcctgaga atgatgtgcg gtttggcgag cgtaaattta gcacccggtt aaacacaatt
      901 aagcatagag gttaaaggag ttaattatgg atgttaccgc aaaatacgaa ttgattgggc
      961 tgatggccta tcctatccgc cacagtttat cgcctgaaat gcagaataaa gccttagaaa
     1021 aagcgggatt gccatttacc tatatggcct ttgaagtgga taacgatagt tttcccggag
     1081 caattgaagg attaaaagcc ctcaaaatgc gcggaactgg tatatcaatg ccgaacaaac
     1141 aactggcgtg tgaatatgtt gatgaattaa cgccagcagc caaactggtg ggtgccatca
     1201 acaccatcgt taatgatgat ggctacctgc gcggctataa caccgacggc acgggccata
     1261 ttcgcgccat taaagagagc ggttttgata tcaaaggcaa aacaatggtg ctgttagggg
     1321 ccggaggtgc ctcaacggcg attggcgcgc agggggcaat tgaaggttta aaagaaatta
     1381 aactctttaa ccgtcgggat gagttcttcg ataaagccct cgccttcgcg cagcgggtta
     1441 acgaaaacac cgattgtgtc gtcacggtta ccgatctcgc cgatcagcaa gcctttgctg
     1501 aagccctggc ttccgccgac attttaacca atggcacaaa agtgggtatg aaaccccttg
     1561 agaatgaatc attagttaat gatatcagtc tgttacatcc gggactgctg gtcactgaat
     1621 gcgtgtataa cccgcatatg acgaagttat tgcagcaggc gcaacaagct ggttgcaaaa
     1681 cgattgatgg atacggcatg ttgttgtggc aaggggctga acagttcacg ttatggactg
     1741 gcaaagattt ccctctggaa tatgttaaac aggtcatggg gttcggtgcc tgacaggctg
     1801 accgcgtgca gaaagggtaa aaaatgaaaa ccgtaactgt aaaagatctc gtcattggta
     1861 cgggcgcacc taaaatcatc gtctcgctga tggcgaaaga tatcgcccgc gtgaaatccg
     1921 aagctctcgc ctatcgtgaa gcggactttg atattctgga atggcgtgtg gaccactttg
     1981 ccgacctctc caatgtggag tctgtcatgg cggcggcaaa aattctccgt gaaaccatgc
     2041 cagaaaaacc gctgctgttt accttccgca gtgccaaaga aggcggcgag caggcgattt
     2101 ccaccgaggc ttatattgct ctcaatcgtg cagccatcga cagcggcctg gttgatatga
     2161 tcgatctgga gttatttacc ggcgatgatc aggttaaaga aaccgtcgcc tacgcccacg
     2221 cgcatgatgt gaaagttgtc atgtccaacc atgacttcca taaaacgccg gaagccgaag
     2281 aaatcattgc ccgtctgcgc aaaatgcagt ccttcgacgc cgatattcct aagattgcgc
     2341 tgatgccgca aagtaccagc gatgtgctga cgttgcttac cgcgaccctg gagatgcagg
     2401 agcagtatgc cgatcgtcca atcatcacga tgtcgatggc aaaaactggc gtaatttctc
     2461 gtctggctgg tgaagtattt ggctcggcgg caacttttgg tgcggtaaaa aaagcctctg
     2521 caccagggca aatctcggta aatgatttac gcacggtatt aactatttta caccaggcat
     2581 aagcaataat atttcggcgg gaacaccctc cccgccgaac ttaaaaaata tatttaaccg
     2641 tatttaataa aaatatttcg tgagtctctg tgcgctaatt ctccatctgg cgtagggaaa
     2701 atcacatctg aatcaggaat taacaatgaa acctgtaaaa ccacctcgta ttaatggacg
     2761 agtgccggtc ctgtcggcac aggaagcggt gaattatatt cccgacgaag caacactttg
     2821 tgtgttaggc gctggcggcg gtattctgga agccaccacg ttaattacag ctcttgctga
     2881 taaatataaa cagactcaaa cgccacgtaa tttatcgatt attagtccga cagggcttgg
     2941 cgatcgcgcc gaccgtggta ttagtcctct ggcgcaagaa ggtctggtga aatgggcatt
     3001 atgtggtcac tggggacaat cgccgcgtat ttctgaactc gcagaacaaa ataaaattat
     3061 tgcttataac tatccacaag gtgtacttac acaaacctta cgcgccgccg cagcccacca
     3121 gcctggtatt attagtgata ttggcatcgg gacatttgtc gatccacgcc agcaaggcgg
     3181 caaactgaat gaagtcacga aagaagacct gattaaactg gtcgagtttg ataacaaaga
     3241 atatctctat tacaaagcga ttgcgccaga tatcgccttc attcgcgcta ccacctgcga
     3301 cagtgaaggc tacgccactt ttgaagatga ggtgatgtat ctcgacgcat tggttattgc
     3361 ccaggcggtg cacaataacg gcggtattgt gatgatgcag gtgcagaaaa tggttaagaa
     3421 agccacgctg catcctaaat ctgtccgtat tccgggttat ctggtggata ttgtggtggt
     3481 cgatccggat caaagccaac tgtatggcgg tgcgccggtt aaccgtttta tttctggtga
     3541 cttcaccctt gatgacagca ccaaacttag cctgccccta aaccaacgta aattagttgc
     3601 gcggcgtgca ttattcgaaa tgcgtaaagg cgcggtgggg aatgtcggcg tcggtattgc
     3661 tgacggtatt ggcctggtcg cccgggaaga aggttgtgct gatgacttta ttctgacggt
     3721 agaaacaggt ccgattggcg gaattacttc acagggtatc gcctttggcg cgaacgtgaa
     3781 tacccgcgcc attctggata tgacgtccca gtttgatttt tatcacggtg gtggtctgga
     3841 tgtttgttat ttgagttttg ctgaagtcga ccagcacggt aacgtcggcg tgcataaatt
     3901 caatggcaaa atcatgggta ccggtggatt tattgatatc agtgccacct cgaagaaaat
     3961 tattttctgc ggcacgttaa ctgcgggcag tttaaaaaca gaaattgccg atggcaaatt
     4021 gcatatcgtc caggaaggac gggtaaataa atttattcgg gaactaccgg aaattacttt
     4081 tagcggaaaa atcgcgctcg aacggggact ggatgttcgt tacatcaccg aacgtgctgt
     4141 attcacgctg aaagaagacg gcctgcattt aatcgaaatc gcccctggcg tcgatttaca
     4201 aaaagatatt ctcgacaaaa tggacttcac cccagtgatt tcgccagaac tcaaactgat
     4261 gaacgaaaga ttatttatcg atgcggcgat gggttttgtc ctgcctgaag cggctcatta
     4321 acaggagtat aatgatggat ttttctttaa ctgaagaaca agaactgctg ctggccagta
     4381 ttcgcgaact gattacgact aactttccgg aagagtattt ccgcacctgc gatcaaaacg
     4441 ggacatatcc gcgtgagttt atgcgggcgc tggcggataa cggtatttcc atgcttggcg
     4501 tgccggaaga atttggtggt atccctgcgg attacgtcac ccaaatgctg gcgctgatgg
     4561 aagtgtcaaa atgcggtgct ccggcatttt tgatcaccaa cggtcaatgt attcacagta
     4621 tgcgccgttt cggttctgca gagcagctac gtaaaacggc agaaagcacc ctggaaacgg
     4681 gtgatcccgc ctatgccctg gcgttgacgg aaccaggtgc tggctcagat aacaacagtg
     4741 ccactaccac ttacacgcgt aaaaacggca aggtctacat caacggacaa aaaaccttta
     4801 ttaccggtgc gaaagagtac ccgtatatgc tggtgttggc gcgcgatccg caaccgaaag
     4861 atcccaaaaa agccttcacc ctgtggtggg tcgactccag taagcccggc attaagatta
     4921 acccgctgca taaaatcggc tggcatatgc tcagcacctg cgaagtctat ctcgacaacg
     4981 tggaggttga agagagcgac atggtgggcg aagaaggaat gggtttcctc aatgtgatgt
     5041 acaactttga gatggagcgc ctgatcaacg ccgcgcgcag caccggcttt gccgaatgcg
     5101 cctttgaaga tgccgcccgc tatgccaacc aacgtatcgc ttttggtaag cccattggtc
     5161 ataaccagat gatccaggaa aaactggcgc tgatggcgat taagatcgac aacatgcgca
     5221 acatggtgct gaaagtggca tggcaagccg atcagcatca gtcactgcgc accagcgcgg
     5281 cgctggcaaa actgtattgc gcacgtaccg caatggaagt cattgatgat gcgattcaaa
     5341 tcatgggcgg tctgggctat accgatgaag cgcgcgtctc ccgcttctgg cgtgatgtcc
     5401 gttgtgaacg tatcggcggc ggtacagacg aaattatgat ttacgtagca ggtcggcaga
     5461 tcctgaaaga ctatcagaac aaataatctg caggcggcgc agcttcttaa caaactgcgc
     5521 cgccagattt atccaacaag acttaccggt tgaggaaatt cagataggat ttcttgttcc
     5581 gcaaaacagt ttttaaattg tctacggtaa tcgctgggcg aacaaccgac atggcgcaaa
     5641 aacagtttgg caaagtgatc gacattttca taacccacac gccaggagat ctctgcctgt
     5701 gataattcag tattagtgag tgaccatttt gcttccgtca tacgccgttg aataacatag
     5761 ttaatagggg aaatacgata ctctttggta aattcatggc aaatataact gacgctggca
     5821 cgaaattttt tcgataactg ttctaaagtg attttttcgc gataattatt attcagatag
     5881 aaaagaatat cttttatcag aacatctttt ttaatatacc cctgctccga acgataagca
     5941 tttttaaaat tttcgtagta aagaatggct aacgtatagg caaatgcatc ccataacgaa
     6001 gatgtttggc tatttttact ttgcggcaaa atcacactta gctcattaaa taaggttttt
     6061 atgacttctt ttccctgccc cgcggcaatt accggacaag aatgcggttg cagtagctga
     6121 ttttcctcag ccccctgaaa ctgaaagccg tacagcgcac aggtacacgt cgttgccgga
     6181 tcgttaacgt cagaggccac cgcatgcagc ctgccgcgtt ctatcaccac aatgtcatcc
     6241 gcatgtgcga catataacga agaatcgatg gtaaaccttg caaccccttt cttgacgtaa
     6301 atcaactcgg tttcattgtc atgaacgtga tgtccggact cccattttgg atcatcgcta
     6361 aatgcaaaac gtgaaagccg tggcgtttta ccggcgacaa acagcgtttc actggcgtta
     6421 tcaaaacagc gttgatacat gacaacctcc ctattccatg agcaagcaaa aacaatatat
     6481 gccggataaa atacggcgtg tattgcggtt atacaaccgc gtttagcgag agctaaacaa
     6541 gattatttac actgaatgca agattgtacg gtcatggaat aactatattg ccgcaaattg
     6601 cacttttgtt ttagcgatcg catttttttt gcaagattgt tggcaaggaa aacagcttgc
     6661 tccgtcgaaa accccgcacc gctatagcac actattttca ggccattttt accttccatc
     6721 ggagatggtt ccgtatgcga ctcacaggag aaatcatgaa aataataacc tgctttaagc
     6781 tggtgcctga agaacaggac attgttgtca ctccagaata caccctgaat ttcgacaatg
     6841 ccgacgccaa aatcagccag ttcgatctca atgccattga agctgcaagc cagctcgcaa
     6901 cagatgacga tgagatagcc gcgctgaccg ttggcggctc attgttgcag aactcgaaag
     6961 tgcgcaaaga cgtgctatcc cgcgggccgc acagcctgta tttggtgcag gatgcgcaac
     7021 ttgaacatgc actgcctctc gataccgcaa aagcgctggc ggcagcaatt gaaaagatcg
     7081 gcttcgattt actgatcttt ggtgaaggtt ccggcgacct ttatgcccag caggttggct
     7141 tgctggtcgg agaaattctg caacttccgg tgattaatgc agtgagtgct attcagcgtc
     7201 agggcaatac actggtgatt gaacgcacgc ttgaagatga tgttgaagtt attgaactct
     7261 ctgttccagc cgtgctctgc gtcacctccg atattaacgt gccacgcatt ccttcgatga
     7321 aagccattct cggcgcgggt aaaaaaccgg taaatcagtg gcaggcaagt gatattgact
     7381 ggagccagag cgcgccactt gctgaactgg tcggcattcg cgtaccgccg caaacagaac
     7441 gtaagcacat cattatcgat aacgattcgc cggaggccat tgccgagctg gcggaacatc
     7501 tgaagaaagc cctgaactga agcctacgga gaagaaacga tgagtcaatt aaacagcgtc
     7561 tgggtcttta gcgataatcc tgaacgttat gctgaactgt ttggcggcgc tcagcaatgg
     7621 ggccaacagg tgtatgccat tgtacaaaat accgaccagg cgcaggcagt tatgccttat
     7681 ggtccaaaat gtatttatgt tcttgcgcaa aacgacgcgc tgcaacgcac tgaaaattac
     7741 gccgaaagca ttgctgccct gctgaaagat aaacaccccg ctatgttgct gttggccgcg
     7801 acgaaacgtg gtaaagctct ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg
     7861 aacgatgcca cggcggtgga tattatcgat ggtcacattt gcgccgaaca ccggatgtat
     7921 ggcgggttag cgttcgctca ggaaaagatc aacagcccgc tggcgatcat tacccttgca
     7981 cccggtgttc aggaaccgtg caccagtgat acctctcatc agtgcccgac agaaacggta
     8041 ccttatgttg ctccgcgtca tgaaattctc tgtcgcgaac gccgtgcgaa agccgcaagc
     8101 agcgtggacc tgagcaaagc aaaacgtgtg gttggcgtcg gtcgtggtct ggcggcgcag
     8161 gatgacctga aaatggtcca cgaactggcg gcggtgctga atgctgaagt cggctgttca
     8221 cgtccaattg ccgaaggcga gaactggatg gagcgtgaac gttatatcgg tgtctccggc
     8281 gtgttgctga aatccgatct ctacctgacg ctggggatct ccgggcagat ccagcatatg
     8341 gttggcggca acggcgcaaa agtgattgtc gccatcaata aagataaaaa tgcgccaatc
     8401 ttcaattatg ccgactacgg tctggtgggc gatatctaca aagtcgtccc tgccctgatt
     8461 agccagttga gccgctaatt cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa
     8521 gggagcataa cgcatgtcgg aagacaaatt tgatgccatt gtggtcggtg cgggcgttgc
     8581 tggtagcgtt gccgcactgg tcatggcgcg agccgggctg gatgtcctgg tgatagaacg
     8641 cggcgacagt gccggatgta aaaacatgac cggcgggcgt ctttatgccc acacacttga
     8701 agcaatcatt ccaggctttg cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa
     8761 aatctccttc ttaaccgaag aaagcgccgt taccctcgat tttcaccgcg agcaaccaga
     8821 tgttccgcaa cacgcatctt ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga
     8881 acaagccgag caggcgggcg cgcagtttat cccaggcgtt cgcgtcgatg cgctggttcg
     8941 tgaaggaaac aaggtcactg gcgtgcaggc tggggatgat attctcgaag cgaatgtggt
     9001 gattctggct gatggcgtta actcgatgct tggccgctcg ctgggaatgg ttcccgcttc
     9061 cgatccgcat cattacgctg ttggtgttaa agaggttatt ggcctcacac cagaacagat
     9121 caacgatcgc tttaatatta cgggcgagga aggtgccgcc tggctgtttg ccggttcccc
     9181 ttctgacggc ctgatgggcg ggggatttct ctataccaac aaggattcca tatccttggg
     9241 gctggtttgt ggattgggtg atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga
     9301 agattttaaa caacaccccg ccattcgccc actaattagc ggcggcaaac tgcttgaata
     9361 ttccgggcat atggtgccag aaggcggtct ggcaatggtg ccgcagatgg ttaacgatgg
     9421 cgtgatgatc gttggtgacg ccgcaggctt ctgcctgaat ttgggtttta cagttcgtgg
     9481 catggattta gccattgcat cggctcaggc tgccgccaca acggtgatcg ccgccaaaga
     9541 acgcgaggat ttctccgcca gcagtctggc gcaatacaaa cgtgagctgg aacaaagctg
     9601 cgtcatgcgc gatatgcagc atttccgcaa gatcccggcg ctgatggaaa acccgcgcct
     9661 gtttagccaa tacccacgaa tggtcgccga catcatgaac gagatgttca ccattgacgg
     9721 caaaccaaac aagccggtac gcaaaatgat catgggacat gcgaagaaaa ttgggctgat
     9781 caacttgctg aaagatggca ttaagggagc aaccgcgcta tgagccagaa cgctacggtt
     9841 aacgtcgaca tcaaattagg cgtcaataaa ttccatgttg atgagggcca cccgcatatc
     9901 attctggcgg caaatcccga tatcaatgaa ttccgtaaat taatgaaagc ctgccctgcc
     9961 ggactttata agcaggatga cgccggcaac attcattttg attctgccgg ttgtctggag
    10021 tgcggcacct gtcgggtgct gtgcggtaac actattctcg aacagtggca atatcccgca
    10081 ggcaccttcg gtattgattt tcgctacggc taatcatgca tcccacaggc ccgcatctcg
    10141 ggcctgatgt tctgtttcga gagtccaaca tgaaagtgac attaacgttc aacgaacaac
    10201 gtcgtgcggc gtatcgtcag caagggctat ggggcgatgc ttcgctggcc gattactggc
    10261 agcagaccgc tcgtgcgatg ccagacaaaa ttgccgtggt cgataatcat ggtgcatcgt
    10321 acaactatag cgcgctcgat cacgccgcga gctgtctggc aaactggatg ttagcgaagg
    10381 gtattgaatc aggcgatcgc atcgcatttc aactgcctgg ctggtgtgaa tttaccgtta
    10441 tctatcttgc ctgcctgaaa atcggtgcgg tttccgtgcc gctgttgcct tcctggcggg
    10501 aagcagaact ggtgtgggtg ctcaataagt gtcaggcaaa aatgttcttt gcaccgacgt
    10561 tgtttaaaca aacgcgcccg gtagatttaa tcctgccgct gcaaaatcag cttccacaac
    10621 tacaacaaat tgtcggcgtg gacaaactgg ctcccgccac ctcttccctc tcattaagtc
    10681 agattatcgc cgacaatacc ccactgacca cggcgataac gacccacggc gatgaattag
    10741 ctgcggtgct gtttacctcc ggaaccgagg gtctgccaaa gggcgtgatg ctaacgcata
    10801 acaatattct cgccagtgag cgggcttatt gcgcgcggct gaatctgacc tggcaggatg
    10861 tctttatgat gcctgcgcca ctcggccacg caacgggctt tctgcatggc gtaacggcac
    10921 cattcttaat tggcgctcgc agcgtgttgt tagatatttt cactcctgat gcgtgtctcg
    10981 cgctgcttga gcagcagcgt tgcacctgta tgctaggcgc aacgccgttt gtctatgatc
    11041 ttttgaatgt actagagaaa caacccgcgg acctgtcagc gctgcgtttc tttctttgcg
    11101 gcggaaccac aatccccaaa aaagtggcgc gtgaatgcca gcagctcggc attaaattat
    11161 taagtgttta tggttccaca gaaagttcgc cgcatgcggt ggtgaatctc gatgatcctt
    11221 tgtcgcgctt tatgcacacc gatggttacg ctgccgcagg tgtagagatt aaagtggtcg
    11281 atgacgcacg caagacctta ccgccaggtt gcgaaggtga agaagcctcg cgtggcccca
    11341 atgtgtttat gggatatttt gatgaacctg aattaaccgc ccgtgcgctg gatgaagaag
    11401 gctggtatta cagcggcgat ctctgccgta tggatgaggc tggctatata aaaattaccg
    11461 gacgcaaaaa agatattatt gtccgcggcg gcgaaaatat tagcagccgt gaagtggaag
    11521 atattttatt gcagcatcct aaaattcacg atgcctgtgt ggttgcaatg cccgatgaac
    11581 gtttaggtga acgatcatgc gcttatgtcg tgctgaaagc gccgcatcat tcattatcgc
    11641 tggaagaggt ggtggctttt tttagccgta aacgggtcgc aaaatataaa tatcctgaac
    11701 atatcgtggt aatcgaaaaa ctaccgcgca ctgcctcagg taaaatacaa aagtttttgt
    11761 taagaaaaga tattatgcgg cgattaacgc aggatgtctg tgaagagatt gaataagttt
    11821 catcttcggg gatcacataa acccggcgac tgaacgccgc cggggattta ttttatttct
    11881 tcagttcagc caggcttaac caggtttgca ccacggtgtc cgggttcaga gacaggctat
    11941 cgatcccctc ttccatcaac catgcggcaa agtcttcatg gtcggacgga ccctgaccgc
    12001 aaatcccgac atatttgccc tgtttcttcg cggcacggat agccatcgac agcaatgctt
    12061 tcaccgcatc gttgcgctca tcgaacaatt cagacaccac gccggagtca cgatccagac
    12121 cgagcgccag ctgtgtcata tcgtttgagc caattgagaa gccgtcgaaa tattcgagga
    12181 actgctctgc cagcaaggcg ttggacggaa tttcacacat catgatgatt ttcagcccgt
    12241 tctcgccacg tttcagcccc tgacgcgcca gttcttcaac caccgctttc gcctgatcta
    12301 cggtacgcac gaacgggatc atgatctcaa cgttggtcag ccccatgtcg ttgcgcactc
    12361 gtttcactgc ttcacactcc agcgcgaagc agtcgcggaa gctgtcggaa acatagcggc
    12421 ccgcgccacg gaagccgagc atcgggttct cttcatctgg ctcgtaacgc tcaccaccga
    12481 ccaggttggc atattcgttc gatttaaaat cagagagacg gacaatgacg cgcttcggat
    12541 aaaacgcggc acccagcgtc gcgatccctt cagtcagacg accaacgtaa aattcacgcg
    12601 gagaatcaaa acctttcatc atctcgcgga tttcgttttg caactgcggt tcctgatcgt
    12661 caaactcaag cagtgcgcgt gggtggacgc caatcatacg gttgatgata aattccagac
    12721 gcgcaaggcc cacgccttcg ttcggcaggc aggcgaagtc gaaagcacgg tccgggttac
    12781 cgacgttcat catcactttc aacggcagat ccggcatcgt ttctacgctg gagcttttca
    12841 cgctaaattc cagcaactcc gcatagacgt aaccggtatc accttcggca caagaaacag
    12901 tgacgttctc accgtctttc atccgttctg ttgcatcacc acagcccact accgccggaa
    12961 tgcccagttc acgagcgatg atcgccgcgt gacaggtacg accgccacgg ttggtgacga
    13021 tggcagatgc tttcttcatg atcggttccc agtccgggtc ggtcatgtca gtgaccagca
    13081 cgtcgccagg ttcgatgcgg ttcatttcgc tgatgtcatg gatgactttc accggacccg
    13141 cgccgatgcg atgaccgata gcacggcctt cggcgataat cttaccctgt gaatgcagcg
    13201 tataacgctc catgacctga ccgcgtgagc gcacggtttc cggacgcgcc tgcacaatga
    13261 acagtttgcc ggtgtggcca tctttcgccc actcaatatc catcgggcga ccgtagtgtt
    13321 tctcaatttg tacggcctgt tttgccagtt cctgcacttc ttcgttggtc agcgagaaga
    13381 tgtcacgctg ttcctgcggt acgtcttcga ttttaacctg cttgccgtgc tcctgggtcg
    13441 gcgcgtaaac catgcggatt tttttcgacc ccatggtgcg acgcacgata gccgggcgat
    13501 tcgctgcaag tgtcggttta tggacgtaaa actcatccgg gttaaccgcg ccctgcacga
    13561 ccatttcacc aaggccccat gcggaagtga taaacaccac ctggtcaaag ccggattcgg
    13621 tatcaatgga gaacatcacg ccagaagatg cgaggtcaga gcgcaccatc cgttgaacac
    13681 cggcggagag cgccacacca cggtgatcgt aaccctggtg cacacgataa gagatggcgc
    13741 gatcgttaaa cagagaagca aatacatgtt tcactgccac gagaacggcg tcaaaaccct
    13801 gaacgttgag gaaggtttcc tgctgaccgg caaaggaagc gtccggcata tcttctgcag
    13861 tagcggagga gcgcaccgca aaagaggcgt tttcgtcatc ggcggaaagc tgtgcataag
    13921 cttcgcggat ggcattttcc agctcaggct ggaagggagt gtcgataatc cactggcgga
    13981 tttgcgcgcc cgctttcgca agctgggtaa catcgtcaat atccgtttta tccagcagtt
    14041 cataaatgcg ctggtttacg ccgctttggt ccagaaactg gttaaacgcg tcggcggttg
    14101 tggcgaaacc attcggaacg gaaacaccca ttccggaaag gttagtaatc atttcaccca
    14161 gggaggcatt tttgccccca accctgtcta catcattcat gccgagttgg ttataccaaa
    14221 gcaccagcgg tgacgagcca ttgttggaca tcgaacaatc cttttgtgat aaatgaacgg
    14281 tttgagaaac acatttctgc gcatttatct ttgcatattt aaccggatga aaaaaacggt
    14341 gaatcgttca agcaaatata tttttttact ttttaagact gatcccagcg ttgcgcaaac
    14401 ctgctctatt cgacaatttc cacataaacc atcggtataa acgaaaccat aaaaatgaaa
    14461 tgctgttttc ataaaaaata aaattgaagg ttcattttat aaaccagaca ttacgtccta
    14521 cgcttctgtg cgtttttaat ttatgctttc atagaattat gtctgcatca cgggaagaac
    14581 aaaatggata atgctgttga tcgccacgtt ttttatattt ctgatggtac ggcaataact
    14641 gcggaggtat taggacacgc agtaatgtca caatttcccg tcactatcag cagcattacg
    14701 ctgccgtttg tcgaaaatga gagccgtgca cgggcagtga aggatcagat tgacgcaatt
    14761 tatcaccaga caggtgtgcg cccgctggtc ttctactcca tcgtgttgcc ggagattcgc
    14821 gccatcatct tgcaaagtga aggcttttgc caggatattg ttcaggcgct ggttgccccg
    14881 ctacaacaag agatgaaact ggatccaacg ccgattgctc atcgtaccca tggccttaac
    14941 cctaataatc tcaataaata tgatgcgcgc attgcggcga ttgattacac cctcgcccac
    15001 gatgacggca tttcgttgcg caatctggac caggctcagg tgatcctgct cggtgtttct
    15061 cgctgtggta aaacccccac cagtctgtat ctggcaatgc agtttggtat ccgcgcggca
    15121 aactacccct ttattgccga cgatatggac aatctggtgc tacccgcgtc gctcaaaccg
    15181 cttcagcata aattgttcgg cctgactatc gacccggaac gtctggcggc gattcgcgag
    15241 gaacgtcggg agaacagccg ctatgcctcg cttcgtcagt gcaggatgga agtcgcggaa
    15301 gtagaagcgt tgtaccgtaa aaatcagatc ccgtggatta acagtaccaa ttattcggta
    15361 gaagagattg ccaccaagat cctcgatatc atggggctta gccgccgaat gtactagaga
    15421 actagtgcat tagcttattt ttttgttatc atgctaacca cccggcgagg tgtgacacac
    15481 ctcgcacttg aaatcagctg cgattggttt atcgtgatgc gcatcacttc ccggtagtcc
    15541 tgccgtagaa gcaacaaatt tctgagactt gtaatgaaca gaactgacga actccgtact
    15601 gcgcgtattg agagcctggt aacgcccgcc gaactcgcgc tacggtatcc cgtaacgcct
    15661 ggcgtcgcca cccatgtcac cgactcccgc cgcagaattg aaaaaatact gaatggtgaa
    15721 gataagcgac tgttggtcat tattggcccc tgctcgatcc acgatctcac cgctgcaatg
    15781 gagtacgcca cccgtctgca gtcgctgcgc aaccagtacc agtcacggct ggaaatcgta
    15841 atgcgcacct attttgaaaa accacgaact gttgtcggct ggaaagggct aatctccgat
    15901 ccggatttaa acggcagcta tcgggtaaat cacggtctgg agctggcgcg caaattactt
    15961 ttacaggtaa atgagctggg tgtccctacc gcgactgagt tcctcgatat ggtgaccggc
    16021 cagtttattg ctgatttaat cagttggggc gcgattggcg cacgtactac cgaaagtcag
    16081 atccaccgtg aaatggcttc ggcactctcc tgtccggtag gttttaaaaa tggtaccgat
    16141 ggcaacacgc ggattgccgt ggatgctatc cgcgcagccc gcgccagcca tatgtttctc
    16201 tcgccagaca aaaatggtca gatgaccatc tatcaggcca gcggcaaccc gtatggccac
    16261 attattatgc gtggcggcaa aaaaccgaat tatcatgccg atgatatcgc cgcagcctgc
    16321 gatacgctgc acgagtttga tttacctgaa catctggtgg tggatttcag ccacggtaac
    16381 tgccagaagc agcaccgtcg ccagttagaa gtttgtgagg atatttgtca gcaaatccgc
    16441 aatggctcta cggcgattgc tggaattatg gcggaaagtt tcctgcgcga aggaacgcaa
    16501 aaaatcgtcg gcggtcagcc gctcacttac ggtcaatcca ttaccgaccc gtgtctgggc
    16561 tgggaagata ccgaacgcct ggtcgaaaaa cttgcctctg cggtagatac ccgcttctga
    16621 atgcgtgccc attcctgatg ggatgggcat ttctgcgcaa cttgttgtct tctcagcaca
    16681 ttactgcttg ctctggtcag ccataatatt gataataaga atcattgtta tatcaattat
    16741 tattaatttt tatgcgttat acggatagca gaaaactcac gcctgaaacg gatgccaatc
    16801 acaagaccgc ttccccgcag cctattcggc gaatttccag ccagacactg ttaggtccgg
    16861 atggcaaact gattatcgat catgacgggc aagaatatct gctccgtaaa actcaggctg
    16921 gcaagctgct gttgaccaag tagcctttaa ctcgagcagc tgacttccag ccgtttaccc
    16981 cagtcaggtg gacgactgac atagtcatca gctctgtcgc tgaaaggatt tcgcaacgcc
    17041 tcatgcaggc ggtgcaattc cgtcatatca cccttttctg ccgcttcaat cgcccgttgc
    17101 gccaaccagt tgcgcaacac cagagctggg ttaacgcttt gcatcagctg ctgacgctca
    17161 ctatcgctaa cctcgtcttg ttgcaaacgc ccccgataac gggcaaacca gtcatcaaat
    17221 gccgcacgat caataaactc atcacgtagc ggtgacgccg cgctgtgctg ctcggtcaga
    17281 ctcagcatgc ggaatgtgcg ggtataatcg ctgcgctctc gcgccatcag actgaataat
    17341 tcattcagta gcgcgttatc ctctttttgc tccgtcatga agcccagttt ctgccgcatc
    17401 cgttgtccat aatgcgtcaa caaaacctgc tgatagctgt ccagggcctc attcagggca
    17461 tctacggcaa caaatggtga caatgtctgc gccagacgct gtaaattcca caacgcgacg
    17521 gcaggttgat tatcaaagct gtaacgccct tgatgatccg agtgattaca aataaaaccg
    17581 ggttcgtaat catcaagaaa accaaacggc ccgtaatcaa gcgtcagccc cagcagcgac
    17641 atgttgtcgg tattcatcac cccatgagca aagccgaccg tctgccattg ggcaattaac
    17701 gatgcggtac gtgcgacaac atcgttaaac cagagacggt atttgtcctc atcatcttca
    17761 agatgtgacc agtaatgacg aatggcaaag tcagctaact gacgaacctt ttccggctcg
    17821 cggcgatagt aaaaatgttc gaaatgacca aagcgcagat gacttggtgc cacacgtatc
    17881 agcatcgcgc ctggctccac cgtttcccga tacactggcg aatcgctggt gacgatactt
    17941 aacgcgcggg tcgtcggaat gcccagataa tgcatcgcct cactggcgag actttctcgt
    18001 atcgtcgaac gtaaaaccgc ccgtccatca cccattcgcg aataaggcgt caggccagca
    18061 cctttcagat gccagtccat tgtagtgcca tcagcaagca gttgttcgcc aagtaaaatg
    18121 ccgcgcccat cacccagttg gcccgcccag acgccgaact gatgaccact gtaaacctgc
    18181 gccagtggtg acataccagg cagtagggtt tcgccgcccc agacacctgc agcattttta
    18241 aacagcgacg atggaatact cagcgtgtta gcgagttcgg tattatgcca aatcagccgg
    18301 gcattattta aaggcgtagg ggaaagtgct gtataggttt ctggcaattc atcgcgccag
    18361 cgggtaacaa aagacagggt catagatcct cctgtttgat agtgtagacg gttactctcg
    18421 tcttaaacac cagcaaacag aagggttatc gctgaaccaa cgtcgttatc tgatttatgg
    18481 gaacggcagg ccacaggcat ccctggagag catggaaatc gaagggggta atctgtgcca
    18541 atatttctgc cgtatcaatg cctcccgcga tgatgcaatt gcaacaaggg gaaatttgcg
    18601 cctggatcgc acgaataaac ggttcgaagg agcggtgcgt gatttgttgc tgaataaaac
    18661 ttttatccag catgacgcgg gtgaaaagac catcaaaaac cgctttcatt gtgctattgc
    18721 ctgctcccaa attacctaac acaaggggat acacttgcga taaggataaa agaccgcgat
    18781 tatcttttcc ttcattgaga tgcgggtaat tctcattaat caacagttca ataaacggat
    18841 attttaatag ttcgcctgcg taattatccc tctctaataa taatgtagcc acttgtggcg
    18901 ttaaattaag ccaggcaaag agtttatgtt gaataaaaaa atgttggcat gattttagta
    18961 attccaactg ctctgaaaat aattgccaat gttgctcctc ggtaagttgc gctataaccc
    19021 gactcgtcgg gatacgcacc gtgccatctt cactggagaa atgggtaatt aactcaacgc
    19081 cgacaaggag ttgctggtta tctcttatcg gcaggaaata gcaatcagaa tgataaagat
    19141 tctccaaaaa aatcttcatg gtaaccgtcc ctctcgaagg atgtttttca gtatccggct
    19201 aaaagggatg aggccataag acataacaat tacagaagga gtaactttca tttgttccat
    19261 gttaaccact ttttcagggg tccttttttt agattatcct gattataaac gaataatcca
    19321 tttacggaat ttttgtctgc aaaatactac tgtattcagg agtaaatgga cggcgatgta
    19381 tcatcgccgt ccatttttcg acatactaaa ttcgtctcgc ctgccagaaa tttttctgcc
    19441 aatagacatt atcaagtgag gaacgcatca ctcccttgct ggtagaggcg tggataaatt
    19501 ggttgttggt atcgtaaata cctacatgca aaccattttg tccggaaccc gttttgaaaa
    19561 agaccaggtc accaggcagc aactcgtctt tatcaatttg cgtgccgata gaggcttgtt
    19621 ctttggtttc tcggggcagc tgcaaatcga aacgatcgcg catcgttaca accacaaatc
    19681 ccgaacagtc cacaccgcgc cgcgtcatgc caccataacg atacggcgtg ccatgccagc
    19741 tttgtagctg gtcgttcaaa ccggcaataa cggtaatcga atcagaaagt ctggcatttg
    19801 gcggcggtgc tttatggtgg ctacacccgg ccagaaacag tgctgtgatc aaaataaggc
    19861 agaaacgcat tccgtacggt tcctctgttt tttattcttg cattaattta gcgtcgtaat
    19921 tacccgattt tcaagatact aatgaaatca gatggtcgaa atcagcattc tgtgaccttc
    19981 gatatccaga cggcgaaaat tcatcccata ggcctgcgcc agatttggcg gcgtgagcac
    20041 ctcttctctg cgtccactgg ccagcatttt tccacctttt agcaaccacg cccgatgcgc
    20101 atgacgcaat gtgtggttga gatcgtgact gctcatcaca atcgccagtc cttgctgaca
    20161 cagagcgctc agaattttgt ctaacgcact ttgttgcgca acatcaagac tgttcatcgg
    20221 ctcatcaaga agcagcaatt gacctgcggg attggcttgt ggtgtgattt gcagcaccac
    20281 cgcagcaaga cgtacgcgtt gccattcacc gccggaaagt tgattggtgc tacgtccgag
    20341 tttgtcatca agagccagcg cccctgcgac atcattcagt agttcggtac gcgttttatc
    20401 gtgctgatgc agtgtcaggt agtgccagac cggcattgca aacggcggcg tctgctgttg
    20461 tgaaagatag gcgcgatgca gcgcgagttt tgttgcggac catgcttcca ggggttgccc
    20521 cgcgaactga atgcttccct taccgctggt cattccggcc attcgtgcaa gcaaggtact
    20581 cttacccgcg ccattcggcc ccaccaggtg caggatctcc ccagcccgaa cctcgccaga
    20641 aagtggcccc aggcgggtag attccgcaac atcctgtaac tgcatcacaa tagacattat
    20701 tttgccaacg ccagtttaat gctttccatc acaatggggt cttccggcgt catatccggg
    20761 gaaaaacgct ggatgaccaa tccgtccctg ccaactaaaa atttctcaaa attccataaa
    20821 atatcatcag ggtacagcgg tgcacgacct ttgctgacca tacgggcata gaatccgctc
    20881 tcttccggcg cgactgcggt cggcgctgcg gcaatcaatt tttgatacag cggatggcgt
    20941 ccttcgccat taacttcaat cttactgaac atcgggaacg tcacccccca tgtggtggta
    21001 cagtaagttt taatctcttc atcgctgccc ggttcttgtt ccagaaactg gttgcacggg
    21061 aatcccagca ccacaaaacc tcgatcggcc caggctttct gaatattctc caactgctca
    21121 tattgcggcg ttaagccaca ctttgaggcg acattgacaa tcaacagcac attaccggcg
    21181 tacttctcca gcgtggtcac ttcaccgtcg atatctttca ctacggtcgt cagaatggaa
    21241 tcttgcatcg tttctcctgg gtgtggtcag taaaaatctt agcttttaat catagaccgt
    21301 ctttttgcgg ctaacgtcct gcttttaaca ataaccagat aaacaccggc gcacccaacg
    21361 ttgcggtgac cacgccaata ggcagctctg cggcagctaa tgccaagcgc gctacaacat
    21421 cggccagcag caatgcgctc gcccctgcca gcgcgcagcc gggaagtaat acgcgatgat
    21481 cggttaaacc acacagccgg agaatatgtg ggatcaccag accaataaag ccgatagcac
    21541 ccgccagcgc cacactgacg ccaaccatcc agccggtcgc tgccaccagc acattgcgcc
    21601 agaaccacag gggtaaaccc agttgccgcg ccgagatctc gccaagtgct aacatattca
    21661 tcggcctgga ctgacaacag atccacaaca acacggggat caatgccagc atcagccagc
    21721 tttgccgcca gtctacgccg ccaaaaccgc ccatcatcca gtacatcagc tgacgcaaat
    21781 caactgaggt ggaaaagtag atagcccacg tcattagtgc gctacagata atccctaatg
    21841 caacgccagc cagcaataac cgactggtcg aaagatgacg acgggcgaaa cgtaagagta
    21901 ttaaagtgat gataagcgcg ccagcaatcg cacacagccc tagcgcccag ttggggagtt
    21961 gcccttgccc aagcaatacc gcggcgataa gccccacgcc tgcgccgtta gagacgccaa
    22021 gtagtccagg ttctgccaga ggattttcaa acaacgcctg cattacagcg ccggatatag
    22081 ccagcgccgc accaaccagc aatacagcca gcgtacgtgg caggcgaatt tgccagacga
    22141 acagttcgcc acgaggagta aaccagtcac ctggcgagat ccattgttca ccggcgcaaa
    22201 ggcttaagag aagcgccagc agcatcaaaa ctgacaggca taataaccag cgaatatttt
    22261 gtcgctgttg ttggcgggca agtgtcagca tggtatccgt tctgctgaag tgtcatggcg
    22321 ttgattttac ggtgactctt cgacagtgaa aagaaaaaag gccgcagagc ggccttttta
    22381 gttagatcag attactcgtc tttgggcgaa gcgttttcga cccggctttt taacttctgc
    22441 ccgggtctga aggtcaccac gcgccgtgct gtaatgggaa tatcctcgcc cgttttcggg
    22501 ttacgtcccg ggcgttgatt cttatcacgc agatcgaagt taccaaaacc agagagtttc
    22561 acctgttcgc cgttttccag agcgcgacgg atctcttcga aaaacagttc aaccagttct
    22621 ttggcatccc gcttgctaag cccaagctta tcaaacagat attctgacat ttcagctttt
    22681 gtaagcgcca taggttcaat ccctcaatga tgcctggaat cgctctttta atgcctctac
    22741 acatttggcg acggtagcgg caatctcctc ttcttcgagt gtacggctgg tatcttgcag
    22801 gatcaggctt atggcgaggc tcttataccc ctccgcaaca cccttaccgc ggtacacgtc
    22861 aaataagttt acgccaacta cctgatttac gccaactttc ttacattcgg ataaaatatc
    22921 cgctgcggga acgttttctg cgaccaccac cgcgatgtca cgacggttcg ccgggaagcg
    22981 agaaatctcg cgcgcctgag gcaccacgcg gtctgcgagc ttgttccact ccagttcgaa
    23041 caccagagtg cgaccgttaa gatccagttt acgttccagt tcaggatgaa caaccccaac
    23101 aaaaccaata cgttcacctt tcagataaat cgctgcggat tgccccggat gcagtgccgg
    23161 attcgcttct gcacggaact caacctcatt cagtttaccg gtcaggtcga gaacggattc
    23221 aagatcgcct ttcaaatcat agaaatcaac ggtctctttt gccaggttcc agtgctcttc
    23281 gtaacggtta ccgcaaatca caccggctaa catcagatcc tgacgaatgc ccaacggtgc
    23341 ttgagtatct ggtacgaaac gcagaccgct ttcgaaaatg cgcacacggt tctgctgacg
    23401 gttctggttg tacaccacgg ttgccagcag gccggtccac agagaaagac gcattgctga
    23461 catttcaaca gagatcgggc ttggcagcag taaggcttca acgcctggat ggatcatctg
    23521 ctgcactttc ggatcaacga agctgtaggt gatcacttcc tgatagcctt tgtcgttgag
    23581 cagcgttttc acgcgcttga gcgacaggtc agcttcacga tgagtaccca taatcaggct
    23641 tgcctgtacc ggctcatccg ggatgttgtt gtagccgtaa acacgcgcga cttcttcgac
    23701 cagatcttct tcaatctcca tatcgaaacg ccagctcggc gcaactgcct gccactcgtc
    23761 tttgccttcg gtcacttcgc agccgagacg acgcagaatg tcagttacct gctcatccgc
    23821 aatatgatgg ccgatcaggc gatccagttt gctacgacgt aaagtgatgg ttgcacgctt
    23881 cggcagcgtt gcttcgctgg tgatatcaat taccggacca gcctcaccac cgcagatgtc
    23941 gatcagcaga cgggtcgcac gttccatcgc tttgtgctgc agtgccggat caacgccacg
    24001 ctcataacgg tgagacgcat cagtatgcag gccatgacga cgagcacgac cggtgataga
    24061 cagcgggctg aagaaagcgc attccagcag gacgttttgc gtttcgtcat tcacgccaga
    24121 gtgttcgcca ccaaagatgc cgcccatcgc cagcgccttg ttgtggtcgg cgatgaccag
    24181 agtgtcagca ttcagcttcg cttcagtgcc atcaagcagc accagcgttt cgccctcttt
    24241 tgccatccgc accacaatgc cgccttcaat gcgatcttta tcgaaagcgt gcatcggctg
    24301 gcccagttcg agcagcacat agttggtgac gtcaacaact gcatcgatag aacggatccc
    24361 gcaacgacgc agtttttctt tcatccacaa cggagttggc gcttcaacgt taatgccttt
    24421 taccacacgg ccaagataac gcgggcaggc ttccggcgct tcgactgtaa tcggcagcgt
    24481 gtcgtcgatg gtcgcaccaa ccggaacgat ttccggttca accagcggca gctggttcag
    24541 cacggcaacg tcacgcgcaa caccaatgat gcctaagcag tcggcacggt ttggcgtcac
    24601 gctgatttcg atggtgttgt catcaagttt caggtattca cggatgtcgg tgccaatcgg
    24661 cgcatccgca ggcagctcga taatgccgct atgatcgtca gaaattccca gctcagaaaa
    24721 ggagcacagc atcccttcag acggttcacc acgcagtttc gccgctttaa ttttgaaatc
    24781 acccggcaga acagcaccaa tggtcgctac cgccacacgc aggccctgac ggcagtttgg
    24841 cgcaccgcag acgatgtcca gcaggcgatc gccgccaaca ttcacttttg tcacacgcag
    24901 tttgtcagcg ttcggatgct gcgcacactc aaccacttca ccaactacca cgccgtggaa
    24961 gctaccggca accggttcta caccgtcaac ttccaggccc gccatagtga tttgatttgc
    25021 cagcgcatcg ctatcaatcg ccgggttcac ccattcgcgt aaccacagtt cactgaattt
    25081 cataatctat tcctgcctta tttaaactgt ttgaggaaac gcagatcgtt ttcgaagaat
    25141 gaacgcaggt cggtgacgcc gtaacgcaac atagtcagac gctccatccc catcccgaag
    25201 gcgaaaccag agtaaacttc cgggtcgatg ccaacgttac gcagcacgtt cggatgcacc
    25261 atcccgcagc ccagcacttc cagccattta ccgtttttac ccatgacatc cacttctgca
    25321 gaaggttcgg taaacgggaa gtaggaagga cggaagcgaa tctgcaaatc ttcctcaaag
    25381 aagttacgca ggaagtcgtg cagcgtgcct ttcaggttgg taaagctgat gttggtatca
    25441 acaatcagac cttccatctg atggaacatc ggcgtgtgag tctggtcgta gtcgttacga
    25501 taaacacggc caggcgcgat gatacgaatc ggtggctgct gggctttcat ggtgcggatc
    25561 tgtacgccag aggtctgggt acgcagcagg cgggtagcgt caaaccagaa agtgtcgtgg
    25621 tcagcgcgcg ccgggtggtg accaggaatg ttcagagcat cgaagttatg ataatcgtct
    25681 tcgatttccg gcccggttgc cacggtaaag ccaagctcac cgaagaaact ttcgatacgg
    25741 tcgatggtac gggtaaccgg atgcagaccg ccgttttcaa tgcgacgacc tggcagagag
    25801 acatcaatcg tttccgccgc cagacgcgca ttcagtgcag cgctttccag ttccgcttta
    25861 cgcgcattca gcgcctgctg aacctgctct ttcgcttcgt tgataaccgc accagctgcc
    25921 ggacgctctt ctggcggcag ctcacgcagg gtcgtcatct gaagggttaa gtgccctttt
    25981 ttacccaaat attcgacgcg cacattatct aacgcggcaa catctgacgc ctggctaatg
    26041 gccgccttcg cactggcaac cagttctgcg agatgtgaca tggttttcct cattgtgtca
    26101 gtggtgacac tggttcgttg gacttagagc ctatcccatc aggctatttt acttgccatt
    26161 ttggtcccgg gcagtgctcg aaatcctcac gtacttaatt acgctccggt ttctccgcgc
    26221 tggccgtgtc cagactggct gcgacaatta cgcctgatag actggctttt tatttttcaa
    26281 aacgcgcata caaaaaaagc ctccactggg aggctttcag gcgctgtttt ccgtttctct
    26341 tctcacgcgc tagcctcctg gattcaggtg ctaaagtaaa aaaagaagcg gaaaatagca
    26401 gcattcattg cttgcgttac cttttggtac tcttcaaaag acctttattg aaaaggctac
    26461 ggcgataaaa gtcaatgttt tgatggcgtt gaaacgaaaa gagggagact agctccctct
    26521 ttcaactggc ttatgccaga gctgctttcg ctttttcaac cagagcggtg aacgctactt
    26581 tgtcgaatac tgcgatatca gccaggatct tacggtcgat ttcaacagag gcttttttca
    26641 ggccattgat gaatttgctg taagaaatac cgttctgacg tgctgctgcg ttgatacgcg
    26701 caatccacag ttgacggaac tgacgcttac gttgacgacg gtcacggtaa gcatactgac
    26761 cagctttgat aacagcctgg aaggcaacgc ggtatacgcg agaacgcgca ccgtagtagc
    26821 ctttagcttg tttcaaaatt ttcttgtgac gtgcgcgtgc aataacacca cgttttacgc
    26881 gagccatatg tgctctcctg tatctatatt ctaattaaaa agttaaaaac gttaacggct
    26941 tatgcgtacg gcaggcacgc gattaccagg cccagatcgc ctttggaaac catggctttc
    27001 ggacgcaggt gacgtttacg tttggtcgct tttttggtca gaatgtgacg caggttagcg
    27061 tgcttgtgct taaaaccacc tttaccggtt tttttgaagc gcttagcagc accgcgtacg
    27121 gtcttaattt ttggcatttt aataacttcc acttcgcatt gttaataaac gaaacaaagg
    27181 cgaacaaagc ctgtgaagcc cgaaggctcc acagacagtg ctacttgaag gccttactgt
    27241 ttcttcttag gagcgagcac catgatcatc tggcggcctt cgatcttcgt tgggaaggat
    27301 tcgaccactg ccagttcttg caaatcgtct ttcacgcgat taagcacttc cataccgatt
    27361 tgctggtgcg ccatctcacg accgcggaaa cgcagcgtga ttttggcttt atcaccctct
    27421 tcgagaaagc gaatcaggct gcggagtttt acctgatagt cgccttcatc tgtaccagga
    27481 cggaatttaa tttccttaac ctggataact ttttgctttt tcttctgttc cttagaagac
    27541 ttgctctttt catagaggaa tttgccgtaa tccattatac gacaaaccgg cggctcggcg
    27601 ttagggctga tctcgactaa gtctactccg gcttcttctg ctttctccag agcttctctc
    27661 agactcacaa taccaagctg ctcgccttcc agacctgtta agcgaacttc ctgggcgcga
    27721 atttcgccat tgatacggtt agggcgcgcc gtttgaactc gttttccgcc tttaatacct
    27781 tattcctcca attgtttaag actgcggctg cgaatctctt gttgcagctt ctcgatcact
    27841 tcatttacgt ccatgcttcc caggtcttta ccacggcggg tgcgaacggc aactttgcct
    27901 gattccacct ctttatcacc acagaccagc atatatggga cgcgacgcaa agtgtgctcg
    27961 cggattttaa agccaatctt ctcatttctc aagtctgctt taacacgaat gcccgcattt
    28021 gatagttttt gcgtcaattc gttaacgtaa tcagactgtg aatcggtaat attcatgata
    28081 acaacctgaa ccggcgcaag ccaggtcggg aagaaaccag cgaattcttc ggtcaggata
    28141 ccgatgaaac gttccatcga cccaagaatt gcgcggtgaa tcattaccgg taccttacgc
    28201 tcgttgtctt cgcccacata ggaggcgctc agacgagacg gcaaggagaa gtccagctgt
    28261 actgtaccgc actgccatgc acgatcgagg cagtcataca gggtaaattc aattttcgga
    28321 ccgtagaaag cgccttcacc cagttgatat tcaaacggga tgttgttttc ttccagcgca
    28381 accgccaggt ccgcctcagc acgatcccac atttcgtcgc tgccaatacg tttttcagga
    28441 cgagtggaga gtttgacgac gatcttctcg aagccaaaag tgctgtacat atcatagact
    28501 aaacggatac atccgttaac ttcatcgcga atttgttctt cagtacagaa gatatgcgcg
    28561 tcatcctggg taaatccacg cacgcgcatc aggccatgca gcgaacctga cggctcatta
    28621 cggtggcagc taccaaactc ggccatacgc agcggcagat cgcgataaga cttcagcccc
    28681 tggttgaaaa tttgtacgtg acccgggcag ttcatcggct taatgcagta ttcacggttc
    28741 tcagaagacg tggtgaacat tgcatctttg tagttgtccc agtgaccggt tttttcccac
    28801 aggacacggt ccatcatgaa cggaccttta acttcctgat actggtactc tttcagttta
    28861 gaacgaacaa acacttctag ttcacggaag atggtccagc cgtcgttgtg ccagaatacc
    28921 atacccggcg cttcttcctg catatggtac aggtcgagct gtttaccgat tttacggtgg
    28981 tcgcgtttcg cggcttcttc caggcgctgc aggtaagcgt taagcgcttt tttgtctgcc
    29041 cacgccgtac cgtaaatacg ttgcaacatt ttgttgttgc tgtcgccacg ccagtaagcc
    29101 cctgccgttt tcattagttt gaaatgatgg cagaaacgca tgttcggtac gtgcggaccg
    29161 cggcacatat cgacatattc ttcatggaag tacagacctg gcttgtcatc atgggcgatg
    29221 ttttcgtcaa gaatggagac tttgtagctc tccccacggt tggcgaaagt ttcacgcgct
    29281 tcgtgccagc tgactttctt cttaatgacg tcgtagtttt tctcagcaag ctcatgcatc
    29341 cgcttctcga gtgcttcgac atcttcctgg gttaacgtgc ggtcaagatc aacgtcgtaa
    29401 taaaaaccgt tgtcaataac cgggccgatt gccattttgg tatgcggcca aagttgttta
    29461 atcgcgtgcc ctaacaggtg cgcacaggag tgacgaatga tctccagacc ttcttcgtct
    29521 ttggcggtaa tgatcgacag ttgtgcgtcg ttttcaatca gatcgcaagc atcaaccagt
    29581 tcgccattaa cgcgccctgc gatacaggct ttcgccagac ctggaccaat gtccagcgca
    29641 acatccatgg ggcttacagc gtggtcgtaa tggcgttggc tgccatcagg aagagttata
    29701 acaggcattt tatatcctta tttgcagtgg tgacccacac gaaagatcac atacaaagaa
    29761 aaatttgttt attaacagtt cattgcgaaa ccatctagcc aacaaatgct aaattggttc
    29821 gcaatcaggt acacaaatga aagacattaa cctctcacct tccacccggt tgagcgatgt
    29881 taacactaaa aaaagggaga ttgtaccttt ccgtttcaca ttgattcgat tcgcaattcg
    29941 tttgtagtga gtacaccagc aaaacgctta aagaaccact gataacatca cggtgcgtct
    30001 gaaaggttta ctgcgagcgg atttacagca gcataaactg aatccagcaa agttcgttgc
    30061 gtgatttggc ggtagtattt aaacgatcat gagattaatg acgaagtggt catatcgcaa
    30121 tgataaaagt gacacaattc ttatagcaat ttttcgtgca catttcgttc tggcgataat
    30181 aattaatcat catcctcaaa acctccagat atataaggcg aacagattat gagtcaaaac
    30241 gatatcatta tcagaactca ttataagtct cctcatagat tgcacatcga tagcgacata
    30301 ccaacgcctt catcagagcc tattaatcaa tttgcgcgcc aactcatcac cctacttgat
    30361 acctctgact taagttcgat gctgtcatac tgtgttactc aggaatttac cgcaagctgt
    30421 cgaaaaatat cacaaaattg ttactccact gcccttttta tcattaactt tgccacttca
    30481 cccatccatg cagaaaatat actcattaca ttacactata aaaaagaaat cattccctta
    30541 tttctggaaa ccacgcctat taaggctaac catttgcgaa gcatactgga ttatattgag
    30601 caggaacagt taactgccga agatcgtaac cattgtatga aactgtctaa aaaaatccat
    30661 agagaaaaga ctatacaacc aacagtaaat ctcaatggta gtgcatgttt tttgcaatct
    30721 ccttctgacg ctattttttg tcgccatctg tcattgcaat acgcccttga ttcattgaga
    30781 aatggaaaag gcaaagtcaa cctgattaaa cattactcct ccgttgaatc catacaacag
    30841 catatcccct taatccggga cgcggagttc agagcattac ttcgccatcc tcctgcaggg
    30901 agtcgcgtta tcgcgagtaa ggattttggc ttcgctttag atattttctt ttgtcgaatg
    30961 atggcaaaca atgtcagtca tatgtccgcg attttatata tagacaatca tactttgtca
    31021 gtaaggctac gaataaagca gtcagtgtat ggacaattaa attatgttgt gtctgtttac
    31081 gacccgaacg ataccaacgt tgccgtcaga ggcacccaca ggacagcacg gggctttctc
    31141 tcgcttgata agttcatcag ttcaggtccc gatgctcaga cctgggctga taggtatgtt
    31201 cgcaactgtg caattgctat tctgccccta ttacctgtgg gagttccagg ggctattttc
    31261 gcgggtattg catcacgaat gccatttgcc cctatacatc catcggcaat gttgttaata
    31321 atggccacag gccagactca acagcttatt acattattca aacagttgcc catactccct
    31381 gaaaaagaaa tcattaaaat aataactgcg cagaatagcg ttggtacacc tgctttattt
    31441 ctggctatga tgaacggaca tgctgacaac gtcaaaatat ttatgcaaga aattcagtca
    31501 ctggtataca atcatatcat tcatgaagat aatctggtta aattgttgca aactaaaagt
    31561 gctaacgaaa cacctggact ctatatctcc atgttgtatg gatttgatga aataatcgat
    31621 atctttctga atgcattaac cactccaata gcacaagagc ttttaaacaa aaaactggtg
    31681 atgagtattt tagccatgaa aatacatgat ggtgagccag gattatatgc cgcaatggaa
    31741 aataatcacc ctttgtgtgt cacacggttc ctctctaaaa ttaatggcat tgcctttaaa
    31801 tacaaattga gcaaagctaa catcatagat ttattaaaag gcgctacagc acatggaacc
    31861 cctgcattat acattgccat gagcaagggt aatgaagacg tcgtgttatc ttatatatcg
    31921 acgctgggta cttttgcaaa aaaacattct tttagtcaac atcagttatt tacactattg
    31981 gctgctaaaa atcatgacaa catgtcagct gttcatatag ccattcatca taatcattat
    32041 aaaactgtag aaacatatta tgctgctata gatataatca gccaaagcct gagttttagt
    32101 gctgatgaac taaagacgta tttataacca actatttacg atatatatgt tgatgcataa
    32161 ttttattttt tcataaaacg cagaaacagt gagcaggtgt acaacgtcac ccaacggctg
    32221 taataacaaa aaatcccttg atgcccgtcc cttttgttac actccgttat cacgcacaag
    32281 agatatgcag gacactggta tgccgactaa acgctttgat aaaaaacact ggaagatggt
    32341 ggtggtgcta ctggcaatct gtggcgctat gttgttgcta cgttgggcag caatgatttg
    32401 gggctaagca gtgacaatcg aaaacagcca gaccgcataa cacggtccgg cagataattt
    32461 tacatgctgt agcccagcga taaggttgtc cgacgatcgg tatgttccgg tgcagactct
    32521 ggaggttcag agttccatgt gacgttgtaa gccaccttca gtccaaaatg ttcattaata
    32581 gcaacattta atgcactttc ggagttcagc gttgtgtctt ccgcgccaaa gaccgaaaca
    32641 ccctgcgtaa atttagcgtt gtcagtcaac tgccatgcat aagcaccgga ggcatagccc
    32701 agcggctgag tttcactggc attgtcggta tatttgtcgt aacgcacacc tggaccgaat
    32761 tcaaagcgga aactgtgtac cgggccattc aaaaactgac gaccataacc cgcggtcaac
    32821 acatcgcgct ctcgatagcc gttataacgg tctgtcagcc agcttgcctg accaaataaa
    32881 tagtcataat cagttaaatt aaaacggcta cgcccgcccg ccgcatattt ttctgaagaa
    32941 cgctcatcat tagaagaagt attactggcg ttcccccaca gcgaccaggc agtggtttgt
    33001 ccataccagg tcatggtggt gtcagccgta agtgaggagc ttttcgtatt gcctgattgt
    33061 gcaagatatc ctgcgttcag attaccttcg aaaggttttt tagcgctggc aggatcgtcc
    33121 atgacagtaa aaacggaatc atcggcagct gcattcagtg acgcaaacat gccccccgcc
    33181 aacataacga tggcaggaac tgtcttcaaa agcttcattt atcaagagtc cgtacaacaa
    33241 aaaaagagac catcgcggtc ccggaaactt tcttaaggat caaagattag cgtccctgga
    33301 aaggtaacga attataaaaa ggcgcgaata acttagcaat gtattcttat ttcatttttt
    33361 gaataagcat gtggcgaaaa cagattttta tttatatata tttatctgca aaattttaaa
    33421 taaagctcta ataaatcata ttgttaattt cttcactttc ggctgattcg gtgccagact
    33481 gaaatcagcc tataggagga aatgatggta cgtatctata cgttgacact tgcgccctct
    33541 ctcgatagcg caacaattac cccgcaaatt tatcccgaag gaaaactgcg ctgtaccgca
    33601 ccggtgttcg aacccggggg cggcggcatc aacgtcgccc gcgccattgc ccatcttgga
    33661 ggcagtgcca cagcgatctt cccggcgggt ggcgcgaccg gcgaacacct ggtttctctg
    33721 ttggcggatg aaaatgtccc cgtcgctact gtagaagcca aagactggac ccggcagaat
    33781 ttacacgtac atgtggaagc aagcggtgag cagtatcgtt ttgttatgcc aggcgcggca
    33841 ttaaatgaag atgagtttcg ccagcttgaa gagcaagttc tggaaattga atccggggcc
    33901 atcctggtca taagcggaag cctgccgcca ggtgtgaagc tggaaaaatt aacccaactg
    33961 atttccgctg cgcaaaacca agggatccgc tgcatcatcg acagttctgg cgaagcgtta
    34021 agtgcagcac tggcgattgg taacatcgag ttggttaagc ctaaccaaaa agaactcagt
    34081 gcgctggtga atcgcgaact cacccagcca gacgatgtcc gcaaagccgc gcaggaaatc
    34141 gttaatagcg gcaaggccaa acgggttgtc gtttcccttg gtccacaagg agcgctgggt
    34201 gttgatagtg aaaactgcat tcaggtggtg ccaccaccgg tgaaaagcca gagtaccgtt
    34261 ggcgctggtg acagcatggt cggcgcgatg acactgaaac tggcagaaaa tgcctctctt
    34321 gaagagatgg tccgttttgg cgtagctgcg gggagtgcag ccacactcaa tcagggaaca
    34381 cgtctgtgct cccatgacga tacgcaaaaa atttacgctt acctttcccg ctaacaaaaa
    34441 cattccccca gcattggggg aatcaccacc aacctgtcgg caacgcgttt ctccgactat
    34501 gctcaaaagt catgtgataa caaaggggtg aactatggcc agtggcgatc ttgtccgtta
    34561 tgtcataacc gtcatgttgc atgaggatac attgactgaa attaacaagt tgaataatta
    34621 cctgactcgc gacggttttt tgctcaccat gacggatgat gagggaaata tccatgagct
    34681 ggggactaac acttttggac ttatcagtac ccaaagtgaa gaagaaatta gagaactggt
    34741 ttcggggctt acccaaagtg caaccggcaa agatcctgaa atcaccatca cgacctggga
    34801 ggaatggaat agcaacagaa aataaatggt ttttgggcaa taatcagtct gtggtgtgcg
    34861 ttagctcgtg tttttacacc gcattcttgc gctaacctta tgatctggca gacaacatgg
    34921 gagagacatc atgtggcagg caatcagtcg tcttttgagc gagcagttag gtgaaggcga
    34981 aatcgaactg cgtaatgaac tgcctggcgg agaagtccat gccgcatggc atttgcgcta
    35041 tgcaggacat gactttttcg tcaaatgtga tgaaagggaa ctgcttcccg gttttaccgc
    35101 cgaagccgac caactggagt tactgtcgcg tagtaaaacc gtcaccgtgc ctaaggtttg
    35161 ggcagtaggc gctgaccgtg actacagttt tctggtgatg gattatctcc cacctcgtcc
    35221 gctggatgcg catagtgcat ttattcttgg tcagcaaatt gcgcgtttac atcaatggag
    35281 tgaccaacca caatttggcc tcgattttga taactcgctc tccacaactc cacagcccaa
    35341 cacctggcaa cgtcgctggt caacgttttt tgctgaacaa cggattggct ggcagttgga
    35401 actggcagca gagaaaggga tcgctttcgg caatatcgac gccatcgtcg agcatattca
    35461 gcagcgtctg gcctcgcatc aaccgcagcc ttctctgttg cacggcgatt tatggtccgg
    35521 caactgtgcg ctgggtccgg atggcccgta cattttcgac cctgcctgct actggggtga
    35581 ccgagagtgc gacctggcga tgttaccgct gcatactgaa cagccgccac aaatctatga
    35641 cggctatcag tcagtatccc cgctacctgc cgatttcctt gaacgtcaac cggtttacca
    35701 actctacacg ctgttaaatc gtgcaagatt atttggcggt cagcatttgg ttattgctca
    35761 gcagtcattg gatagattat tagcagcatg atatgggttg aggataatgg ccgctccgtg
    35821 cggccttttg attaaataaa cccaagcaga gaaaagaaca cataacctgc agcaatacaa
    35881 ataaccggca ggatatatag tgagaaaaac tgcaggaaaa tagtatggtg cggaacaaca
    35941 atacgggact caatttgttc acgcgttagc ccctcttccc ctttggcttt ttccagaatg
    36001 agttgatctt caacaccttc ccgcaagaag cgcgcctggc gactcatccg ggcaccggaa
    36061 tcctgcaacg ccagtccgac aaaaatcagg atgaaaatca cccagaacat aacgttcacg
    36121 ccaccattaa aattaggggt cggggagtta taccagaaca gattcaaaaa aggcgtattc
    36181 gcctgcatca tatcgatcat gacatgagta aagtcgagca tgaccgcatt aatgccttcc
    36241 tggttttcct gccgggtatt cataaatttc agcaaagaaa tcaatgtaga gatcaatgca
    36301 ggtataaaaa tcacccaacc caaaatcctt ttcaaaacag caatgcgtcc agcttgttga
    36361 tacgtcatga gttctccttg attaagacgc gtcgtttcac ttagtttacc tgtagatatc
    36421 tgttttcgcc cattctttaa aggcgatatg ataggcgctt aatcataagc acggcttaat
    36481 accttacaca taatgctcta aaggagaggt tgtaatgtca accccgcgtc agattcttgc
    36541 tgcaattttt gatatggatg gattacttat cgactcagaa cctttatggg atcgagccga
    36601 actggatgtg atggcaagcc tgggggtgga tatctcccgt cgtaacgagc tgccggacac
    36661 cttaggttta cgcatcgata tggtggtcga tctttggtac gcccggcaac cgtggaatgg
    36721 gccaagccgt caggaagtag tagaacgggt tattgcccgt gccatttcac tggttgaaga
    36781 gacacgtcca ttattaccag gcgtgcgcga agccgttgcg ttatgcaaag aacaaggttt
    36841 attggtggga ctggcctccg cgtcaccact acatatgctg gaaaaagtgt tgaccatgtt
    36901 tgacttacgc gacagtttcg atgccctcgc ctcggctgaa aaactgcctt acagcaagcc
    36961 gcatccgcaa gtatatctcg actgcgcagc aaaactgggc gttgacccac tgacctgcgt
    37021 agcactggaa gattcagtaa atggcatgat cgcctctaaa gcagcccgca tgcgttccat
    37081 cgtcgttcct gcgccagaag cgcaaaatga tccacgtttt gtattagcaa acgtcaaact
    37141 ctcatcgctg acagaactca ccgcaaaaga ccttctcggt taatggccag gggcagtgat
    37201 ctcgctgccc ctggttcttt atctgaattt gcattcaact gacggattaa tcgtcaattt
    37261 aagagaaaga gttacaccgt caccacttcc gtgcactgta taaaaatcct atactgtacg
    37321 tatcgacagt ttagtgagtt ttatcatgac ggcggaaggt caccttctct tttctattgc
    37381 ttgtgcggta tttgccaaaa atgccgagct gacgcccgtg ctggcacagg gtgactggtg
    37441 gcatattgtc ccttccgcaa ttctgacgtg tttgttaccg gacatcgatc acccaaagtc
    37501 gtttcttggg cagcgattaa agtggatatc aaaaccgatc gcccgcgctt ttgggcatcg
    37561 tggttttacc cacagtctgc tggcggtatt tgcgctgctg gcaacctttt accttaaggt
    37621 tcctgaaagc tggttcattc cggctgatgc gctacaagga atggtgctgg gttatttaag
    37681 ccacatactt gccgatatgc tgacacccgc cggtgttccc ctgctctggc catgccgctg
    37741 gcgtttccgc ttgcctatcc tggttcccca aaagggcaac caactggaac gttttatctg
    37801 catggcatta tttgtctggt cggtatggat gccccattca ttacccgaaa acagcgctgt
    37861 ccgttggtca tcgcaaatga tcaatacctt gcagatccag tttcatcggc ttattaagca
    37921 tcaggttgaa tactaaaaag gcaaaaatca cctttctgga ataagcaatt ccatttgaat
    37981 ataagagcca gctcacagtt ctgttaatct tgcgccaaca ctatgactgc tacgcagtga
    38041 tagaaataat aagatcagga gaacggggat gaactttcca ttaattgcga acatcgtggt
    38101 gttcgttgta ctgctgtttg cgctggctca gacccgccat aaacagtgga gtctggcgaa
    38161 aaaagtgctg gtgggtctgg tgatgggtgt ggtttttggc cttgccctgc ataccattta
    38221 tggttctgac agccaggtac ttaaagattc tgtacagtgg tttaacatcg ttggtaacgg
    38281 ctatgttcaa ctgctgcaaa tgatcgttat gccgttagtc ttcgcctcta ttctgagcgc
    38341 ggttgcccgt ctgcataacg catctcagtt aggcaaaatc agttttctga ccatcggtac
    38401 gcttttgttt accacgctga ttgcggcgct ggtcggtgtg ctggtcacca acctgtttgg
    38461 tttgacggct gaaggtctgg ttcagggtgg tgcagaaact gcacgtctga acgccatcga
    38521 aagtaactat gttggtaaag tctctgacct gagcgttccg cagctggtct tgtcctttat
    38581 cccgaaaaac ccgtttgccg atctgaccgg agccaatccg acgtcaatta tcagcgtggt
    38641 aatttttgcc gcattcctcg gtgtagctgc gctgaaactg ctgaaggatg atgcgccgaa
    38701 aggtgaacgc gtcttaaccg ctatcgatac cctgcaaagc tgggtgatga aactggttcg
    38761 cctggtcatg cagttgaccc cttacggcgt tctggcactg atgaccaaag tggttgcagg
    38821 ttctaacctg caagacatca tcaaactggg aagtttcgtt gtcgcgtcct acctcggtct
    38881 gctgattatg tttgcagtgc atgggattct gctgggcatt aatggcgtga gtccgctgaa
    38941 gtacttccgt aaggtatggc ctgtgctgac gtttgccttt accagccgtt ccagtgctgc
    39001 gtctatccca ctgaatgtgg aagcacaaac gcgtcgtctg ggcgttcctg aatccatcgc
    39061 cagtttcgcc gcctctttcg gtgcgaccat tggtcagaac ggctgcgccg gtttgtatcc
    39121 ggcaatgctg gcggtgatgg ttgcgcctac ggttggcatt aacccgctgg acccgatgtg
    39181 gattgcgacg ctggtcggta ttgttaccgt tagctccgca ggcgttgccg gtgtcggtgg
    39241 tggtgcaact ttcgccgcac tgattgtgct gcctgctatg ggcctgccag taaccctggt
    39301 ggcgctgtta atctccgttg aaccgcttat cgacatggga cgtacggcgt taaacgttag
    39361 tggctcgatg acagctggca cgctgaccag ccagtggctg aagcaaaccg ataaagcaat
    39421 tctggatagc gaagacgacg ccgaactggc acaccgttaa ttcttatgct ggcaacggcc
    39481 cgttttgtat aggggccgtt gccttacttt ttaaatgttc catcgcggcg atgcatactg
    39541 actcatattt aaacagatcg tcagcgaaaa aaatgtccaa cttatcaata cattcctggc
    39601 tcaatgaatg tgcctgtagc gagaagctta aaacctcata cccgagacgt tttctaagtg
    39661 aattatctgg aatactcata atatcttcaa gcacaccact tcccattccc ggaagattta
    39721 gtggattaaa aagaagattg atattaagca ttgatattat cgaaggaaac tttaggagac
    39781 tttcattttt tgaatcgcag caaaaatgct cccggcagga tgtatataat tttcttcata
    39841 gtatatagtt cttaaatagt gaataaccaa cctgtttttt atattgatca aattggtcat
    39901 tttttctggg cgaaatacag aagtgacatc cattccaata aaatctttat ttatacctct
    39961 tatccgaatt tcactaccat aaagattatt aattattact ctatatttaa cattgtcctg
    40021 cagatatgta tcaaaactaa gtgctggcgc tcctggcttt gaggcatcat agttaatgtt
    40081 agacatatag gcaatcatac gattgaagtt tgattctgct atttcatcgc agtcacattg
    40141 acccgttgat ataaagaaag tgagtgccgc catggtagac tcaagccttt ctttaaaaga
    40201 gtcaaaagcc cgacttttaa tatttaaatc accataatta ctctgtatta agtttgtaga
    40261 aaacatctcc cacctcatat tgttaacaaa actattatct catttaaatc taagtcattt
    40321 acaatataag tttaagcgcg acgccacagg atgaactatc aaaaatagct catcatgatt
    40381 agcaaaactt aaccatttta aaataaataa acaattaaag aaaaaagatc acttatttat
    40441 agcaatagat cgtcaaaggc agctttttgt tacaggtggt ttgaatgaat gtagcaatga
    40501 aatacagaat ttcaggtcat gtaactcccg gcaaaaccgg gaggtacgta atctttactc
    40561 agtcacttcc ccttcctggc ggatctgatt tgcccaacgc tgggcggatt caggcacagt
    40621 aaacgccggt gagcgcagaa atgactctcc catcagtaca aacgcaacat atttgccacg
    40681 cagcatccag acatcacgaa acgaatccat ctttatcgca tgttctggcg gcgcgggttc
    40741 cgtgcgtggg acatagctaa taatctggcg gttttgctgg cggagcggtt tcttcattac
    40801 tggcttcact aaacgcatat taaaaatcag aaaaactgta gtttagccga tttagcccct
    40861 gtacgtccca ctttgcgtgt atttcataac accgtttcca gaatagtctc cgaagcggga
    40921 tctggctgat ggtctatagt tagagggttt tttgaccaaa acagcggccc tttcagtaat
    40981 aaattaagga gacgagttca atgtcgcaac ataacgaaaa gaacccacat cagcaccagt
    41041 caccactaca cgattccagc gaagcgaaac cggggatgga ctcactggca cctgaggacg
    41101 gatctcatcg tccagcggct gaaccaacac cgccaggtgc acaacctacc gcccctggga
    41161 gcctgaaagc ccctgataca cgtaacgaaa aacttaattc tctggaagac gtacgcaaag
    41221 gcagtgaaaa ttatgcgctg accactaatc agggcgtgcg catcgccgat gatcaaaact
    41281 cactgcgtgc cggtaaccgt ggtccaacgc tgctggaaga ttttattctg cgcgagaaaa
    41341 tcacccactt tgaccatgag cgcatcccgg aacgtattgt tcatgcacgc ggatcagcag
    41401 ctcacggtta tttccagcca tataaaagct taagcgatat caccaaagcg gatttcctct
    41461 cagatccgaa caaaatcacc ccagtatttg ttaggttctc taccgttcag ggtggcgctg
    41521 gctctgccga taccgtgcgt gatatccgtg gttttgccac caagttctat actgaagagg
    41581 gtatttttga cctcgttggc aataacacgc caatcttctt tatccaggat gcgcataaat
    41641 tccccgattt tgttcatgcg gtaaaaccag aaccgcactg ggcaattcca caagggcaaa
    41701 gtgcccacga tactttctgg gattatgttt ctctgcaacc tgaaactctg cacaacgtga
    41761 tgtgggcgat gtcggatcgc ggcatccccc gcagttaccg caccatggaa ggcttcggta
    41821 ttcacacctt ccgcctgatt aatgccgaag ggaaggcaac gtttgtacgt ttccactgga
    41881 aaccactggc aggtaaagcc tcactcgttt gggatgaagc acaaaaactc accggacgtg
    41941 acccggactt ccaccgccgc gagttgtggg aagccattga agcaggcgat tttccggaat
    42001 acgaactggg cttccagttg attcctgaag aagatgaatt caagttcgac ttcgatcttc
    42061 tcgatccaac caaactgatc ccggaagaac tggtgcccgt tcagcgtgtc ggcaaaatgg
    42121 tgctcaatcg caatccggat aacttctttg ccgaaaacga acaagcagca ttccatccag
    42181 gtcatattgt tcctggtctg gacttcacca acgatccgct gttgcaggga cgtttgttct
    42241 cctataccga tacacaaatc agtcgtcttg gtgggccgaa tttccatgag attccgatta
    42301 accgtccgac ctgcccttac cacaacttcc agcgtgatgg catgcatcgt atggggatcg
    42361 acactaaccc ggcgaattac gaaccgaact cgatcaacga taactggccg cgcgaaacac
    42421 cgccgggacc gaaacgcggc ggttttgaat catatcagga acgcgtggaa ggaaataaag
    42481 ttcgtgagcg cagtccatcg tttggcgaat attattccca tccgcgtctg ttctggctaa
    42541 gtcagacgcc attcgagcag cgccatattg tcgatggttt cagttttgag ttaagcaaag
    42601 tcgttcgtcc gtatattcgt gagcgcgttg ttgaccagct ggcgcatatt gatctcactc
    42661 tggcccaggc ggtggcgaaa aatctcggta tcgaactgac tgacgaccag ctgaatatca
    42721 ccccacctcc ggacgtcaac ggtctgaaaa aggatccatc cttaagtttg tacgccattc
    42781 ctgacggtga tgtgaaaggt cgcgtggtag cgattttact taatgatgaa gtgagatcgg
    42841 cagaccttct ggccattctc aaggccctga aggccaaagg cgttcatgcc aaactgctct
    42901 actcccgaat gggtgaagtg actgcggatg acggtacggt gttgcctata gccgctacct
    42961 ttgccggtgc accttcgctg acggtcgatg cggtcattgt cccttgcggc aatatcgcgg
    43021 atatcgctga caacggcgat gccaactact acctgatgga agcctacaaa caccttaaac
    43081 cgattgcgct ggcgggagac gcgcgcaagt ttaaagcaac aatcaaggtc gctgaccagg
    43141 gtgaagaagg gattgtggaa gctgacagcg ctgacggtag ttttatggat gaactgctaa
    43201 cgctgatggc agcacaccgc gtgtggtcac gcattcctaa gattgacaaa attccggcgt
    43261 aaatatggat aaggtcacgt cccctgggga gtgaccttac caccttactt acacatcaag
    43321 ataactaccc aaccgataac cacgctcagc aattgcatat ttcaacgacg ctgatgtcag
    43381 cacttccagc tccgttaacc gtgggaaaca gtaagcactc tgacggatag tattatcgat
    43441 aaacgccgga tgacacatca cctccagcga acgatcaccc cgatggcttg cgtcatccag
    43501 cacttgcagg aacagtgcct cgctaatctc ttcaccataa aacgcgctac tgaatccctg
    43561 agaactgcgc agattagccg gtaaatcacc agcgttagat agcggctgac ggtcgatgcg
    43621 caacgcaatc ccctcttcag ccgcaaacct ggcaacaatc gggaaaatct gcgggaacat
    43681 atgcacatga tgatggctat caagatgcgt aggtttgcgt ccaaatagct caatgaaacg
    43741 caaatactga ctggcaagct cctgagtaat ttcttccagc ggtaaagcat cttcttctgc
    43801 caactgccag atccattttc ccagcacacc atcgcgggtt aaccccggca tagctgtcag
    43861 tggcttgccc atagtaagga caaagtgcat ccctatggcc agactcggtt catcacgact
    43921 caactgcacc gcatggtcaa tagcctgccc attcaccagc gccgtcgttg atgtgacaat
    43981 cccattgcga caggcctcga taatgccgta gttctgacct ttgcttaagc caaaatcatc
    44041 ggcattaaca atcagtaagc gttccataat cagcctcggt taatgtgctt ttttaagctc
    44101 tgcgatgcag tcggcaaaat ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc
    44161 cagcagctca gcatcgcgat cggaatgcac caacggacta aggtttagcg ccagtaacac
    44221 atcgttaaat tctccgctaa gtgcggcgtt gctggcagca atctcgaagc ctttaatggt
    44281 gtgaatcagc cccatcactt tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc
    44341 atcgcgcccc agcttacagg tcatttctac cgcccagtct gccggaatat tatcaatatg
    44401 cccatgatgc gggatattaa cgtaatgttc agcttgcttg tcgttgtaga tagcgttgat
    44461 cacttcgcac gctgcatcag agtaataagc cccaccgcgc tgttccagtt ctttcggctt
    44521 aactttcaac tccggatttt tatacagctc aaaaagttgt ttctcgactt tctgtactac
    44581 ctgtgctcgt gcgccgcctt tgtagtattc gcccatttca atagccagca tctctttctg
    44641 cttgaagtaa tacagcagat aagaacatgg cagcagattt aacgaacgaa ttaagccctc
    44701 actaaatggc agatcgaaaa tattttttac agaggacgct tttaactgcc ctgacgccac
    44761 accatcaagc aattcggcaa agcgcgactt gccatttacc agcacatcct taatgaacac
    44821 catatggttg aggccgaaca gatcgataga taaatcatcg ctgtctttca gcatcagaac
    44881 atcgcgaata aacatcttca tgccgatcgg aatattacac acgccgataa agcgtttaaa
    44941 tccggtatga cgataaacgg cttcagtgac cattcccgcc gggttagtga agttaatcac
    45001 ccatgcattc ggacaaagtt cttcgacatc ttttacgatg tcaaaaatca ccggaatggt
    45061 acgcagacct ttaaacagac cgcccgcgcc gttggtttcc tgaccaagat aaccatgact
    45121 taatggaata cgttcatcca gttcgcgcgc cggtaattgg cctacgcgca gttgggtagt
    45181 aacgaaatca gcatctttca atgcttcgcg gcgatccagc gttttataaa gcttcatcgg
    45241 gacgccagcg ttatcaatca tccgttggca gagttcaaaa atgatatcca gtttctcttt
    45301 accatcttcg acatccacca gccataattc gctgaccggc aattcgtgat aacgcttaat
    45361 aaatccttcc agtaactccg gggtatagct gctcccgcca ccaatagtga cgacttttaa
    45421 tttctggctc ataatttctc ccttcagtac agaatactga tatctggcat atctgccccc
    45481 ccggacataa ataatccagc aacaggacag atatgtgaat tgtcaggtat aacgacttac
    45541 tgattaaatt cagtcaattt cttacgatag ctcttaggcg taaaggacgt taatttctta
    45601 aacgttttaa taaacaaact cgggctacta taaccggcct caaacgcaat atccgtcacg
    45661 gaatagttgg tcatttccag ttgttttttg gcaaaattaa tacggatctc attaataatc
    45721 tgcattggtg ttttgccata atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg
    45781 gctgacaacg tcaccatatt ctccagcgcc gattcactaa actgctcttt atcatgcatc
    45841 ttttctaccg tacttttcag ccactgcggt acatcatcaa tcacctgttc ttcgcggtaa
    45901 tgacgtaaac ggttaatgac ataaaaagta accatctcaa caaactcttc cagccctgtt
    45961 tcgcggaaat tcaatgaaga aatcactgtt tccacatagg tgagaaaagc gttattggtc
    46021 cggtatacct gcgaagcgac aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa
    46081 aagcgtttac tgatcccaac gttcaatata cgcgtggcac caaactcata aaaactttga
    46141 tggtgcgaac ctaacggaat aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg
    46201 ttaatctcct ggaaataacg cccggttaat accagagtaa attcataata gtcgtgctga
    46261 tgcagcccgc tgatactctc agttttgtta tagataaaca catggaagtt tttgccatta
    46321 aacaactgct gttctcgggc agtggcaatt tccggcgcgt taatcactgg ctgcatcatc
    46381 gactccttat gccttcagtt tttcatgaag ctcaattaat tcagtaatca gttcacgcgc
    46441 aagcatggac gtcattaaat gatcctgagc gtggaccagc accagactca ctttcatctt
    46501 accttcgccc gcatcgcctt caatcagttt cgtctgtacc agatgcgctt cattcaatgc
    46561 catccgtgac tgatccatca tggcttttgc tgcagcaaaa tcgccctgct tcgcctgttt
    46621 cagtgccgca tacgccaggc tgcgcgcttg tccggagttg atgataagcc ccatcaccac
    46681 ttcttccagc tcttcagctt ccgtttgcgt atcgggaatg ttatcgagat ccatcataca
    46741 tcgttcctct ctattcttac cggcacgatt acccgtaccg gcatcgatta aaatttcagg
    46801 gcgttagcga tatcttcttc gctctcttct ttatcaatcg cgttttgtgc tttgttagcc
    46861 accacaacaa agggcagata aattaacgtt gcgataccaa ggttgaagag tgcgaccagc
    46921 aatgcggcga cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac
    46981 ggtgcaatat tggtcaccgg aggaatgatg cccatgtagt acgctgcgag ggtgattgcc
    47041 gccagaatcg gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc
    47101 agaccaaaca gaatcggttc gttaatctgg aagatgccgg acggcagcgc cagttttgcc
    47161 acctgacgat aatcagcacg gcgagaggcg ataaagatag ccaggatcag gcctaaagtc
    47221 gcaccactgc ccccaaggaa aataaaggag tccagcatcg gcttggccca gatatggaag
    47281 gtcttaccgg ctgccagcgc cgcttcgacg gaatcatatt gctgataggt cgcgatattt
    47341 tccagcgccc acggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcgccatga
    47401 ataccgaaga accagagcag tggaacaaag atcacatagg cccagcccac cacgctaccc
    47461 aacgatgcca gtggggttga gatggtatcc ataatgatct gatggaagtt ggtgccccag
    47521 gtattcaacg cccaggcaat aatccccatc acggaaagaa taataaagcc gggaattaat
    47581 gccgagaagg aacgcgatac tgaagcaggt acgctatcgg gcaatttaat gacccaattg
    47641 cggcggacaa taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg
    47701 atattcgctc cacctaacca gtttgcgcca accgcatagg cctcaccgac actatatggg
    47761 gtgacggtca taaatgctgc aacggataac aacccagccg ccagcgcatc gactttacgc
    47821 tcttctgcca gcgccatgcc aataaagaac ggtgccatta aagacattat ccctaatgtt
    47881 ccgttatata cgttgccgcc aatacctttc aggccattaa gtgtttcaat ggttgaggca
    47941 tctaggcgaa tacctaagga ataaaaaaat gacccctccc caaagcttag aaaaacgttg
    48001 ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat aaagccattt
    48061 ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag gagtaccttt
    48121 tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca atttaaatat
    48181 tgcggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa ttaatttgct
    48241 gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca
    48301 taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc
    48361 aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca
    48421 gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg
    48481 cgcatttttg ataccagtaa agaggtagac atgcccgcag aacaaaacag ataaatgtgt
    48541 ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc
    48601 ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga
    48661 aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa attgtttctc
    48721 cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa
    48781 gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc aagctttcag
    48841 acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc
    48901 ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc
    48961 acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc tcacttcaga
    49021 cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt
    49081 caccacaggc tgtacaaact ggtctttggt acgatcataa gccgtacaac ccgccagcat
    49141 ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct
    49201 tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgtgaac
    49261 gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg
    49321 tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg
    49381 aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt
    49441 aatgctgaag aggaaattcg tcgtagtatc gattttctga aaagctacct gcaaacttat
    49501 ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccgga
    49561 aagctgtgcc agatggcgat taatgagctg cgcctggaaa ccggcaacga atcactgcaa
    49621 tttattgccg tacgcctgcc ctatggtgtt caggccgacg aacaagattg ccaggatgcc
    49681 attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggcc
    49741 agcgaacagg cattgcggga agcaggcatt gaactgagcg attttgtccg tggcaatgaa
    49801 aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg
    49861 gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc
    49921 ggtacagaca ttaatccgct gtatcgtctc aacaaacgtc agggtaaaca gttactggcg
    49981 gcattaggtt gcccggaaca cctttataag aaagcgccaa cggccgatct ggaagatgat
    50041 cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat
    50101 ctggaaggga aaaacgtacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa
    50161 accgaacata aacgccgtcc gccaattacc gttttcgatg atttctggaa aaagtaataa
    50221 tttgcacata atggtttgtg cgaaaaagag taatttgttc acgccggatg cggcgtgaac
    50281 gccttattcg acctacaaaa ctatgcaaat tcaatatatt gcaggagtcg aataggcctg
    50341 acaggcgtag cacgtcagac ggtgtaacct ttgtcatcga cccgcctctt ttttaatcgc
    50401 ttcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt
    50461 aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttacg
    50521 ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga
    50581 caccatgccg ctctatatcg gcaaaagcat taacatccgc agccgcgtcc tttctcattt
    50641 acgtaccccg gatgaagccg ccatgctacg gcaatcccga cgcatcagct ggatctgcac
    50701 cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac aacagccgct
    50761 gtttaataaa cggttgcgcc gcaatcgcca gctctgcgcc ctgcaattaa atgaaaagcg
    50821 cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct
    50881 gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg aacaaaaact
    50941 ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc
    51001 gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac atgcgctacg
    51061 cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gggcggtggc
    51121 gctgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct
    51181 gggggcggtt aattcgctgg aagacgcgac aacgttaatt cggacacccg ccgggtttga
    51241 tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga
    51301 acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttaccttgc
    51361 ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg
    51421 agcaatttat cgcccagttg ccacgccccg ttaatcacaa acaccacccc gccgcgtgag
    51481 ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgt
    51541 gtcatgatgt tgaaatccat cgacatctgc cctgccgtca gtttcgcttt taccacctgg
    51601 tccgccgcaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt
    51661 tcaaggaaca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc gggaaacaaa
    51721 gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt
    51781 gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca cagattcacc
    51841 gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta
    51901 tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggtgatca
    51961 ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt
    52021 actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag attattcagc
    52081 agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct caaaattagc
    52141 attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct gggtttccat
    52201 gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg cttcagcttt
    52261 tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt cttccagcgg
    52321 cggacgtttc atctggtcac gctggccttt catgatttcg cggatctgct gtttctgcgc
    52381 gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact tgcctttgtg
    52441 gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg catgggccag
    52501 gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt tacgcatatt
    52561 ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc gtgttgacga
    52621 gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca atgaaagtgt
    52681 aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag agtgatgaga
    52741 gaataataca acacgatgat tttgcagaga ttatgaagaa ctataccgga tgactggtga
    52801 taaataaagc aaataaccag gattaatctg tattaattta taagaaagca acttaatacc
    52861 cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa tcccgcgcgt
    52921 aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg ggttacggta
    52981 aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat aaaattcagc
    53041 gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg cgaaaccacc
    53101 cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag cagcgcagca
    53161 attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg caacgctttg
    53221 ccaagttcca gcgtacaggc cagcgcgcca aaatgtctgg cgctgaaatg ggtaaacgta
    53281 ccaccaggtt cctgatggaa caccagcgcc tccagccccg ccgcacccag ccacgtcaga
    53341 aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg atgcaaggag
    53401 ccacgaattg cggtatgtag atcaaggtgc cagcgcacag attctttgcc caggtcataa
    53461 aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct ttcagcaaat
    53521 agctgccaac gaccgccaaa cattcgattc atatcgctat ggcaataacg tttcccttgc
    53581 ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg gatctcgccg
    53641 tgagatatcg cgccaagcaa cgcgtccagc atctccacag gtgccgtctc attaccgtgt
    53701 attcccgctg aaatcaccag tgcgccttgc ggtggcgtta atggcgtcag ttccagcaca
    53761 ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat aaccggtttt
    53821 ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc gctggaacgg
    53881 ataaaccgaa ccgagattca gtaattggct caatacatcc agtgcttccc gccctccgcg
    53941 cagcaattgc gggtcggcca gatcggcagc agtaaggcga tcgcggtagt aacgatccac
    54001 ccagtcattg agcgcattaa acagcgtatc gttcatcatc accgccggat tcaccgcccg
    54061 gcgttcttct tctgtcaata ccacccgcaa ccgcaggcac gccgggccgc cgccattcgc
    54121 catgctttca cgtaaatcaa agacttttag ttcgctaatc gggttgtcag cggcaaggag
    54181 ttcattgaga taaccccata ctccggcgtg ttcccgacac tcctgaggca gcaccaacat
    54241 catggaacca tcatcgcggc tcaacagttg gctgttaaac agataggtag acaccgcatc
    54301 cgacacggaa acctgagttg ccggaacttc tatcgccata aaaccgttca cccgcgcacg
    54361 caggtttgcc agtaactgtg actggcgagc gaacgcctgt tggtggcaaa acagcacctg
    54421 gcggttactc acggcaatca cgtcattatg aaaaacgccc tggtcgataa cgtccgggtt
    54481 ttgctgggcg aaaatcacct gttggggatt cacctgattc agccttgcca ccgcctcgct
    54541 ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg gtatcattgc cttcttctcg
    54601 cccgtagaca aaaagttgca tacccggctc accgtaatga ccgccgagac gattgtggtt
    54661 tgccgccccc tcatcaccga gcaacgctac ctgcggcaac gccgaatgaa cgctaaattt
    54721 ctcttcgtcg ttaaaaatcg cttttaaaag tgattcggtg acgtgcgctt ccagcgagcg
    54781 gtgaaattta ttgttcaggt tggcaacggt gagatgcact ttgccatcca gcgtatcggc
    54841 agatggcgcg atcgttgccg cattggctac ccacattggc gaagcggaac tgacgctgga
    54901 aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc tgctcatcgc taccactgaa
    54961 tcccaactga cgcagcaccg gaataaacgg acgttcgtgc ggcgggatca cggcctgggg
    55021 gaatcccgca tcggcaaggg ctttcatttt cagtaagccc tgttttgccg cctgtcgcgg
    55081 gttagacacc tgaaaacggt gacgggtaga ggcttcatta ccaaacgaca ggccagcgta
    55141 atgatgcgtc agccctacca gcccgtcgaa attaacttcc caggcgttca tcgcaccacc
    55201 tcatcggaaa aatccagccc ggggttaagc gttgcgggca aagttaacga gtccgactcc
    55261 aggctcgcca taggccatgc gcagtaatct gcggcatacc aggcgctggg gcgatggtta
    55321 ccggatgcac caatgccgcc gaatggcgcg gtactggcag caccagtaag cggtttgttc
    55381 cagttaacaa tccccgcccg cgcctccagc aacagttgat cgaatttttc ccgctcgggg
    55441 gaaaccagac cgcaagagag tccgaagcga gtgttattcg ccattagaat cgcttcctcg
    55501 aaagaatcat aacgccagac gcgcaataac ggtccgaaca cctcttcatc tggtacgcca
    55561 gcaacgcctg tcatttcaat gatccccggc gtcagcaacg atgtctctga ttgtaataag
    55621 cgcggcgcaa gcagggttcg tccgcccatc gcttccagtt gctgccaggc agtaaccacc
    55681 tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg gctgcggttc gtcatcccag
    55741 ttgcccggcg ttaatcgctg gctgacggca accagacgag caagaaacgc atcgccctgc
    55801 gctccgcttt tgagcaataa acggcgggcg caggtgcagc gttgcccggc tgtgacaaac
    55861 gccgactgaa tggtcagatg gacagccgcg tcgatatccg ccacctcatc gataattagc
    55921 ggattattac cgcccatctc aagggcgaga attttctccg gctgaccgga gagctggcga
    55981 tgcagctggt agccggtatt ggcgctaccg gtaaacagca aaccgtcgag atcctccagc
    56041 gcactcagcg cctgacccgt ttcacgcccg ccctgcacca ggttaagtac gcccggcggc
    56101 aagccagcct gctgccataa acgcattacc gcttcgccac tccacggtgt cagttcgctg
    56161 ggtttaaaga taatggtgtt acctgccagc aatgccggaa cgatatgtcc gttcggcaaa
    56221 tgaccaggga aattatacgg cccaaacacc gccagcacgc cgtgcgggcg atgtcgcagg
    56281 ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg tacgaacgtg atacgcctta
    56341 attgatatcg cgattttatt gatcatcgcc gtcacttcgg ttgccgcttc ccagcgcggc
    56401 ttacccgttt ctctggcaat aatcgcggtt aattcgcctt tattcctttc cagcaatccg
    56461 gcaaagcgtt cgacaacgac ctgacgttca gccaatgaga gccgcgccca gcgcggaaac
    56521 gccgcacggg ctgcgcgaca agcctgcccg acctgagtgg catcggcatc attgccttgc
    56581 cataacacct cgcccgatac cggattacgc ttcacacgcg atgcgccctg gcccgttatc
    56641 cagtcaccgt taatccataa agtcatgctg ttttctcctc tgcgcacagg cgcaccagac
    56701 gaacgcgatc ccctgcgtgg catttgaggg catccagttg tgcggcggtt aaaatcaaac
    56761 gctcggttgc cggatcggta cgcgccagca ccacgcggaa atggtgataa ttttcattgg
    56821 cgaccaggca ggccggaaaa tcgccctgcg caggctgccc ttctgccact tccaccagcc
    56881 gacttttacg aatggcgcgc acgcggtcga tgtcacactc aagcgttggc ccgccgtcga
    56941 agatgtcgat atagttgcgg taacgaaaac cttctttctc cagcaccgcg cgggcaggcg
    57001 cggtttgcgg atgtacctga ccgatgacgt cctgggcttc ctgggataaa aagtgggtat
    57061 agatcggatg tttcggcatc agttctgcaa taaatgcctt ttgcccggtg ccgcagagaa
    57121 aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc gaggctttgc cagaacggtg
    57181 aatagccgtg ttcgtcaatc accccgcgca tttcagcaac cactttgtca ttaaacttgt
    57241 cgcgaaaagc cgccataaac ataaagcgcg atttcgacag caaatagccg ttgccctctt
    57301 tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct gctgccggtg tgatcgttac
    57361 tgagaaacag cgtcggcaat gcgttataga cattcagctc ttttgaggcg tgaaccaacg
    57421 tgccgacgcg atagttgtac cagggatcgt tcagcccaac cgccacctca atggcacaaa
    57481 tccccgccac ggtgcctgtc tcgctatctt ccagcacgaa cacatagccc tgctcacttt
    57541 tgggcagttc gccttgccag gttttgattg ccctttcgat acgcgccgaa agcgtggctt
    57601 cattggcggg aagcgacgtc aggccgccgc ccgttttgct ggcaagctgc atcagcgccg
    57661 agacatctga tcgctcaacg ggacggatga ccatcatgat gaacctcggc taacaaagtg
    57721 ttcgcaagca gctgcaaagc gatccagtcc ggtcgtcacc tcttcttcgc tgacattgag
    57781 cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt accatcacgc ctgctttcgc
    57841 cgcttcctga gagatctgtt tcgcttgccc ggcgtaatcg gcattcagta cacagccaat
    57901 cagcaaacct aagccgcgaa cttcactgaa caaaccatag cggtgattaa tagtattaag
    57961 acgctcaaca aaccagtcgt gacgctgttt aacgccatta agcatctctg gtgtgttgat
    58021 gagctccagc actttgcctg ccaccgccga ggccagcgga ttaccgccat aggtggtgcc
    58081 atgagtgcca acggtcatca cgcgggcgca ctcttcggtt gccaacaacg caccgaccgg
    58141 gaaaccgccg cccagcgctt tggcggtagt taacagatca ggcgtcacgc cgtagtgcat
    58201 ataggcatac agttccccgg tgcgcccgac gccggtttgt acttcatcaa aaatcaacag
    58261 cgcattgtgg cggttacaca attcacgcag accttgtaaa aacgcgttgc tggctggcac
    58321 cacaccgcct tccccctgga tgggttcgac aatcaccgca caggtagagt cgtcaatcag
    58381 cgcgctggca gagttaatat cgttatatgc agcatgacga atatccgccg gcagtggcgc
    58441 aaaatcctgt gaataggctg gctgcccacc cgcactgaca gtaaacagcg tgcgaccatg
    58501 aaacgcattt ttgaacgcca cgatgccgct cttatggctg ccgtagcggt catgagcgaa
    58561 tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca ccggagttac aaaagaagac
    58621 gcgatcggca aacgtggcgt cgatcaattt tttcgccagt cgcagtaccg gctcgttggt
    58681 gtaaccgttg ccggtatgcc agaacttact cgcctgttcg ttcagcgcct cacgcagttc
    58741 cggatgcgca tggcccagcg cgttcaccgc aatgccaccc gcgaagtcga tatactcttt
    58801 cccctgctga tcccacaagc gcgaaccttc gccacgtacc ggtataaagg gtgccggagc
    58861 gtaaacaggt atcatccatt catcaaagtt ttcacgcgta attggctgag acatagcgac
    58921 ctctacagta aataattcgt tatttatatg ttaacaataa gtaatgtttg cgctgtaaat
    58981 gtagattgca gggttcgtgc cagccagtga taaaagtgca taaacggcgg aggctaactg
    59041 gaaatcaagg agttataacc aaaccatatg catttaaagt gcatataaag tgaatacgtt
    59101 tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt atgcagaagt aaaatataat
    59161 tctggaattg tgatcattga cgaaatttac tggaaattac tgcgccattc tgacgcagcg
    59221 cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc attaacaacc atcgatcaaa
    59281 tcacttaaca acaggcggta agcaacgcga aattctgcta ccatccacgc actctttatc
    59341 tgaataaatg gcagcgacta tgaaatttgt ctcttttaat atcaacggcc tgcgcgccag
    59401 acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg gatgtgattg gcctgcagga
    59461 gacaaaagtt catgacgata tgtttccgct cgaagaggtg gcgaagctcg gctacaacgt
    59521 gttttatcac gggcagaaag gccattatgg cgtggcgctg ctgaccaaag agacgccgat
    59581 tgccgtgcgt cgcggctttc ccggtgacga cgaagaggcg cagcggcgga ttattatggc
    59641 ggaaatcccc tcactgctgg gtaatgtcac cgtgatcaac ggttacttcc cgcagggtga
    59701 aagccgcgac catccgataa aattcccggc aaaagcgcag ttttatcaga atctgcaaaa
    59761 ctacctggaa accgaactca aacgtgataa tccggtactg attatgggcg atatgaatat
    59821 cagccctaca gatctggata tcggcattgg cgaagaaaac cgtaagcgct ggctgcgtac
    59881 cggtaaatgc tctttcctgc cggaagagcg cgaatggatg gacaggctga tgagctgggg
    59941 gttggtcgat accttccgcc atgctaatcc gcaaacagca gatcgtttct catggtttga
    60001 ttaccgctca aaaggttttg acgataaccg tggtctgcgc atcgacctgc tgctcgccag
    60061 ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac tatgaaattc gcagcatgga
    60121 aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc cgctaattta gcagctctcc
    60181 tggctcaaac tgggtcagga gaattaacct tgagaaaaat caacaaactg tcagtaatga
    60241 tttgttgcct gccgtccttt gttataccgt cactgcgttt ttagttgtct gaccacttct
    60301 ctattatcaa gtttgatata ggaaactcca cgatgaacgc tgagcgtaac tttctgtttg
    60361 cctgtcttat ttttgcgctg gtcatttacg ctatccacgc tttcggttta ttcgatctgc
    60421 tcaccgattt accccactta cagacactca ttcgccagag cggattg
//
LOCUS       DAEVZA010000012        58010 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEVZA010000012 DAEVZA010000000
VERSION     DAEVZA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.012"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1600
                     /locus_tag="LAF92_000803"
     CDS             <1..1600
                     /locus_tag="LAF92_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBI9789856.1"
                     /translation="LIPLVFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDV
                     WSALEQLLKQGPMDIYPARIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRF
                     TQGGRFYSGLEQLKTFCQPQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTA
                     RDLTTQILAWPQEGKSARAVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFL
                     QVVAENRGLSQEELADRLVPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVR
                     QKSVPRLRADDDQLKAPEALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHT
                     LFVNHPFTRLVTQRLIWGVYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGI
                     AHPLEMTAEMRSEFAQLFADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVG
                     QLMGMRYKGWESGYEDAFVYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNK
                     SVTFAELDVFDLSEALSAPDVIFH"
     gene            1661..1981
                     /locus_tag="LAF92_000804"
     CDS             1661..1981
                     /locus_tag="LAF92_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789857.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
     gene            3164..4252
                     /locus_tag="LAF92_000805"
     CDS             3164..4252
                     /locus_tag="LAF92_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBI9789858.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            4263..6542
                     /locus_tag="LAF92_000806"
     CDS             4263..6542
                     /locus_tag="LAF92_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789859.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRLREAFMARWIGWAMQQNNGDVLVVCGGWHAPVLAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            6535..7671
                     /locus_tag="LAF92_000807"
     CDS             6535..7671
                     /locus_tag="LAF92_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBI9789860.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSCIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            7668..9668
                     /locus_tag="LAF92_000808"
     CDS             7668..9668
                     /locus_tag="LAF92_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBI9789861.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCTTAQPTEKEE
                     AWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFANDEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            9793..10254
                     /locus_tag="LAF92_000809"
     CDS             9793..10254
                     /locus_tag="LAF92_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBI9789862.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(10295..10765)
                     /locus_tag="LAF92_000810"
     CDS             complement(10295..10765)
                     /locus_tag="LAF92_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBI9789863.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(10812..11531)
                     /gene="yehT"
                     /locus_tag="LAF92_000811"
     CDS             complement(10812..11531)
                     /gene="yehT"
                     /locus_tag="LAF92_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBI9789864.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(11528..13213)
                     /gene="btsS"
                     /locus_tag="LAF92_000812"
     CDS             complement(11528..13213)
                     /gene="btsS"
                     /locus_tag="LAF92_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBI9789865.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            13435..14166
                     /gene="mlrA"
                     /locus_tag="LAF92_000813"
     CDS             13435..14166
                     /gene="mlrA"
                     /locus_tag="LAF92_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBI9789866.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14226..14333
                     /locus_tag="LAF92_000814"
     CDS             14226..14333
                     /locus_tag="LAF92_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBI9789867.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(14314..15045)
                     /gene="yehW"
                     /locus_tag="LAF92_000815"
     CDS             complement(14314..15045)
                     /gene="yehW"
                     /locus_tag="LAF92_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBI9789868.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(15050..15976)
                     /gene="yehX"
                     /locus_tag="LAF92_000816"
     CDS             complement(15050..15976)
                     /gene="yehX"
                     /locus_tag="LAF92_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBI9789869.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(15969..17126)
                     /gene="yehY"
                     /locus_tag="LAF92_000817"
     CDS             complement(15969..17126)
                     /gene="yehY"
                     /locus_tag="LAF92_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBI9789870.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFQHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(17133..18050)
                     /gene="osmF"
                     /locus_tag="LAF92_000818"
     CDS             complement(17133..18050)
                     /gene="osmF"
                     /locus_tag="LAF92_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBI9789871.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(18261..20558)
                     /gene="bglX"
                     /locus_tag="LAF92_000819"
     CDS             complement(18261..20558)
                     /gene="bglX"
                     /locus_tag="LAF92_000819"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBI9789872.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            20754..22469
                     /gene="dld"
                     /locus_tag="LAF92_000820"
     CDS             20754..22469
                     /gene="dld"
                     /locus_tag="LAF92_000820"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBI9789873.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(22507..23439)
                     /gene="pbpG"
                     /locus_tag="LAF92_000821"
     CDS             complement(22507..23439)
                     /gene="pbpG"
                     /locus_tag="LAF92_000821"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBI9789874.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(23613..24200)
                     /locus_tag="LAF92_000822"
     CDS             complement(23613..24200)
                     /locus_tag="LAF92_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBI9789875.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            24370..24948
                     /locus_tag="LAF92_000823"
     CDS             24370..24948
                     /locus_tag="LAF92_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI9789876.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(25078..25839)
                     /locus_tag="LAF92_000824"
     CDS             complement(25078..25839)
                     /locus_tag="LAF92_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI9789877.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(25892..27328)
                     /gene="mdtQ"
                     /locus_tag="LAF92_000825"
     CDS             complement(25892..27328)
                     /gene="mdtQ"
                     /locus_tag="LAF92_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBI9789878.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            27552..27635
                     /gene="yohP"
                     /locus_tag="LAF92_000826"
     CDS             27552..27635
                     /gene="yohP"
                     /locus_tag="LAF92_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBI9789879.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(28008..28955)
                     /gene="dusC"
                     /locus_tag="LAF92_000827"
     CDS             complement(28008..28955)
                     /gene="dusC"
                     /locus_tag="LAF92_000827"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBI9789880.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            29194..29592
                     /locus_tag="LAF92_000828"
     CDS             29194..29592
                     /locus_tag="LAF92_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBI9789881.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            29589..30284
                     /locus_tag="LAF92_000829"
     CDS             29589..30284
                     /locus_tag="LAF92_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBI9789882.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            30414..31298
                     /gene="cdd"
                     /locus_tag="LAF92_000830"
     CDS             30414..31298
                     /gene="cdd"
                     /locus_tag="LAF92_000830"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBI9789883.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            31448..32167
                     /gene="sanA"
                     /locus_tag="LAF92_000831"
     CDS             31448..32167
                     /gene="sanA"
                     /locus_tag="LAF92_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBI9789884.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            32170..32409
                     /locus_tag="LAF92_000832"
     CDS             32170..32409
                     /locus_tag="LAF92_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBI9789885.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            32728..33966
                     /gene="preT"
                     /locus_tag="LAF92_000833"
     CDS             32728..33966
                     /gene="preT"
                     /locus_tag="LAF92_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBI9789886.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            33960..35195
                     /gene="preA"
                     /locus_tag="LAF92_000834"
     CDS             33960..35195
                     /gene="preA"
                     /locus_tag="LAF92_000834"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBI9789887.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(35438..36448)
                     /gene="mglC"
                     /locus_tag="LAF92_000835"
     CDS             complement(35438..36448)
                     /gene="mglC"
                     /locus_tag="LAF92_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBI9789888.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(36464..37984)
                     /gene="mglA"
                     /locus_tag="LAF92_000836"
     CDS             complement(36464..37984)
                     /gene="mglA"
                     /locus_tag="LAF92_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBI9789889.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(38045..39043)
                     /gene="mglB"
                     /locus_tag="LAF92_000837"
     CDS             complement(38045..39043)
                     /gene="mglB"
                     /locus_tag="LAF92_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBI9789890.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(39323..40363)
                     /gene="galS"
                     /locus_tag="LAF92_000838"
     CDS             complement(39323..40363)
                     /gene="galS"
                     /locus_tag="LAF92_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBI9789891.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(40505..41662)
                     /locus_tag="LAF92_000839"
     CDS             complement(40505..41662)
                     /locus_tag="LAF92_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBI9789892.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(41679..42347)
                     /gene="folE"
                     /locus_tag="LAF92_000840"
     CDS             complement(41679..42347)
                     /gene="folE"
                     /locus_tag="LAF92_000840"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBI9789893.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            42605..43441
                     /gene="yeiG"
                     /locus_tag="LAF92_000841"
     CDS             42605..43441
                     /gene="yeiG"
                     /locus_tag="LAF92_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBI9789894.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(43473..45464)
                     /gene="cirA"
                     /locus_tag="LAF92_000842"
     CDS             complement(43473..45464)
                     /gene="cirA"
                     /locus_tag="LAF92_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBI9789895.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(45757..47226)
                     /gene="lysP"
                     /locus_tag="LAF92_000843"
     CDS             complement(45757..47226)
                     /gene="lysP"
                     /locus_tag="LAF92_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBI9789896.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(47431..48312)
                     /locus_tag="LAF92_000844"
     CDS             complement(47431..48312)
                     /locus_tag="LAF92_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9789897.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            48411..49460
                     /locus_tag="LAF92_000845"
     CDS             48411..49460
                     /locus_tag="LAF92_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBI9789898.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            49534..50391
                     /gene="nfo"
                     /locus_tag="LAF92_000846"
     CDS             49534..50391
                     /gene="nfo"
                     /locus_tag="LAF92_000846"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBI9789899.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            50395..51483
                     /locus_tag="LAF92_000847"
     CDS             50395..51483
                     /locus_tag="LAF92_000847"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBI9789900.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(51539..52789)
                     /locus_tag="LAF92_000848"
     CDS             complement(51539..52789)
                     /locus_tag="LAF92_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBI9789901.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNKIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNISFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(52889..53830)
                     /gene="rihB"
                     /locus_tag="LAF92_000849"
     CDS             complement(52889..53830)
                     /gene="rihB"
                     /locus_tag="LAF92_000849"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBI9789902.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            53960..54658
                     /gene="yeiL"
                     /locus_tag="LAF92_000850"
     CDS             53960..54658
                     /gene="yeiL"
                     /locus_tag="LAF92_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBI9789903.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(54726..55976)
                     /locus_tag="LAF92_000851"
     CDS             complement(54726..55976)
                     /locus_tag="LAF92_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBI9789904.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(56070..57008)
                     /gene="psuG"
                     /locus_tag="LAF92_000852"
     CDS             complement(56070..57008)
                     /gene="psuG"
                     /locus_tag="LAF92_000852"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBI9789905.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(56996..57937)
                     /locus_tag="LAF92_000853"
     CDS             complement(56996..57937)
                     /locus_tag="LAF92_000853"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBI9789906.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
BASE COUNT        14244 a        15379 c        14406 g        13981 t
ORIGIN      
        1 acttatccct cttgtcttta ccaaacccag cgataatagc gaagccgcat tacttgccct
       61 gcgtttactg tacgaacagg gacatggcga attgctacaa accgtggcaa accgctggca
      121 gcgtacagat gtatggtctg ccctggagca gttgcttaaa cagggtccaa tggacattta
      181 cccggcacgc attccaaaag cccctgattt ctggcatccg gcaatgtggt ccaggcctcg
      241 acttatcact aataatcagc ctgttaccgg tgacgctctg gaaattatcg gcgaaatgct
      301 gcgctttacc caggggggac gtttttatag cgggctggaa caactgaaaa cgttctgcca
      361 gccacaaacg ctggcagctt ttgcctggga tctcttcact gcgtggcaac aagctggtgc
      421 ccccgcaaaa gacaactggg catttctggc gttaagtctc tttggtgacg aaagcacggc
      481 acgggatctg acgacacaga tcctcgcctg gccacaagaa ggcaaatctg cccgtgctgt
      541 cagcggcctg aacatcctta ccctgatgaa taatgatatg gcgctgatac agctgcatca
      601 tatatcgcaa cgggctaaat cccgcccctt acgtgataac gcggcggaat ttcttcaggt
      661 ggtcgcagaa aatcgcgggc taagccagga agagctagcg gacagattag tcccaaccct
      721 gggccttgat gatccgcagg cgttgagttt tgattttggt ccccggcagt ttaccgttcg
      781 cttcgatgaa aacctcaacc cggttatctt tgatcagcaa aacgttcgcc agaaaagcgt
      841 tccccggttg cgcgccgatg acgatcaact gaaagcgccc gaggcactgg cccgactaaa
      901 agggctaaaa aaagatgcta ctcaggtgag caaaaacctg ctcccgcgtc ttgaagctgc
      961 cctacgtacc acccgacgct ggtcgctggc agattttcat actctgtttg ttaatcatcc
     1021 ctttacccgc ctggttaccc agcgattaat atggggcgtg tatccggcaa atgaaccgcg
     1081 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaggatga
     1141 gccaattgac ctgcctgcgg atgctctgat tggcattgcc cacccgttag aaatgacagc
     1201 agaaatgcgc agtgaatttg cccagctttt tgccgattac gaaattatac cgccacttcg
     1261 ccagttgaca cggcgcacgg tgctgctcac gcctgacgag tcagccagta atagtttgaa
     1321 tcgctgggaa ggtaaatctg ctaccgttgg gcaacttatg ggaatgcgat acaaaggctg
     1381 ggagtcaggc tatgaggacg catttgtcta tgacctgggt gagtaccggc tggtccttaa
     1441 gttttcaccc ggttttaacc actacaatgt tgatagcaaa gcgctaatga gcttccgttc
     1501 tcttcgagtg tacagtgaca ataaatccgt cacttttgcc gaacttgatg tgtttgattt
     1561 gagtgaggcg ttaagcgcac ctgacgtcat tttccattaa aacacagcgg gcagtgatgc
     1621 aactgcccgt tatcaacacg acttaccagc ggggaaagcg atgatcgtgc aaaaagagct
     1681 ggttgctatt tacgattatg aggtccctgt acctgaagat ccgttttcct tcagacttga
     1741 gatccataaa tgctctgaat tatttacagg ttccgtctat cgactggagc gattccggct
     1801 acgtccaaca tttcatcaac gtgatcgaga agatgctgac ccgctaataa atgatgcgtt
     1861 gatttatata agagatgagt gtattgatga gcggaaatta cgaggtgaat cacctgaaac
     1921 tgtaatagca atttttaatc gtgaactaca gaatatattc aaccaactaa tagaatatta
     1981 gtttattaaa gggggaagcg gttttccccc ttagttgcgt ttcaatttgc ccattacttc
     2041 aacaaatcaa tcaaccgtaa cagagccact aatagctcca gtacggcaat gagcacggtt
     2101 aacttttgtg tcagcgtgtc catccgctct ccttattaag gagcgcaaca ccagcacctt
     2161 acccttacac tgcctgtcga tactggttta cgtttgttga gtcgccatgt tgcactccgg
     2221 atcagagcac tacctgcggc accacccgtc cggtagccag gacttgaaat gggttcgatc
     2281 cggcgcaact tcccaacagt caggattata aatctcatta tcttaattgc aacactgtcc
     2341 agaggtggtt ataattcgtt cgttgagatg ccatgttgca ctatcagtaa cttaacatca
     2401 aagtagtaaa taaaagaacc tgcctcacca gcaggttttt ttattttctg cgatctactt
     2461 tccagatatt ttttgccaat agcccaccat aaatattcac atcccggtca aatggtgcgt
     2521 tgaatataaa atcgatgaat ttataaataa aaatgagtta tatgatgaat taataaaaac
     2581 agtaatcatt ttattaaagg gggaagcggt tttccccctt agatgcgttt caatttgccc
     2641 gttacttcaa caaatcaatc aaccgtaaca gagccactaa tagctccagt acagcaatga
     2701 gcacggttaa cttttgtgtc agcgtgtcca tccgctctcc ttattaagga gcgcaacacc
     2761 agcaccttac ccttacactg cctgtcgata ctggtttacg tatgttggac cacgatgttg
     2821 cactccggat cagggcacca cctgcagcac cacccgtccg gtagccagga cttgaaatag
     2881 attcgatccg gcgcaacgtc ccaacggtct ggattataaa tctcattatc ttaattgcaa
     2941 cggggtccag ccgtggttat aattcgttcg ttggaacacg atgttgcaca ggctgtggtg
     3001 ttaggcctga aagtagtaaa taaaagaacc tgcctcacca gcaggttttt ttatttactg
     3061 tgatctgctt tccagatatt tttcgctcaa acaactaatg cgccaaacat ttattgcgcg
     3121 taaaatatcg tttatttcat taatacattt cagggatgaa tatatgtcac cgcagaataa
     3181 tcatctgcag cgtccgcctg ctgctgtgtt atacgccgat gaactggcaa aattaaaaca
     3241 aaatgataac gctccttgcc cgcccggttg gcagttaagt ttgcctgcgg cccgtgcttt
     3301 tatccttggc gatagcgcgc aaaatattag ccgtaaagtg gtgattagtc cctccgctgt
     3361 cgaacgtatg ttagtcactc ttgccaccgg acgcggtttg atgttggtgg gggaacctgg
     3421 caccgcaaaa tctctcctgt ctgaattact ggcaaccgct attagcggcg atgccgggtt
     3481 aaccatccag ggcggcgcat ctaccactga agatcaaatc aagtacggct ggaactacgc
     3541 tctgcttatc aatcatggcc catcaacgga agcgctggtc cctgccccgc tttaccaggg
     3601 aatgcgcgac ggcaaaatcg tccgttttga agagataacg cgtacaccgc tggaagtgca
     3661 agactgtctg ttaggtatgc tttctgacag ggtgatgacg gtgccggaac tcactggtga
     3721 agccagtcag ctctatgcgc gagaaggctt taatattatt gccactgcca atacccgaga
     3781 tcgcggcgtc aatgaaatga gtgctgcgct aaagcgtcgc ttcgattttg aaactgtctt
     3841 tccgattatg gatttcgccc aggagctgga gcttgtcgcc agtgcttcgg cgcgtttgct
     3901 ggcccatagc ggtattccgc ataaagtgcc tgacgcggta ctggagttac tggtccgcac
     3961 cttccgcgat ctgcgtgcca acggcgaaaa gaaaacgtca atggatacac taacagcgat
     4021 tatgtccacc gctgaagccg tgaacgtggc ccacgccgta ggcgtccgcg cctggttttt
     4081 agcgaatcgc gcaggtgagc cagccgacct ggtggactgc attgccggaa ccatcgtcaa
     4141 agataacgaa gaagatcgcg ctcgtctgcg ccgttacttc gaacaacgag tcgccacaca
     4201 taaagaagct cactggcagg cttattatca agcccgccac cgcctgccgt gaggaaagat
     4261 gcatgagcga gccgttaatt gtcggcatcc ggcatcatag tccggcctgc gcccggctgg
     4321 tgaaatcgtt aatcgaaagc cagcggccac gatacgtatt gattgaaggc ccggctgatt
     4381 ttaatgaccg ggtagacgaa ctgtttttag cccaccagct tccggtagct atttacagtt
     4441 attgccagta tcaggacggt gcagcccccg ggcgtggtgc ctggacgcca tttgctgaat
     4501 tttcgccgga gtggcaggcg ctacaagccg cacgtcgtat tcaggcacaa acttacttca
     4561 tcgatttgcc ttgctgggcg cagagtgaag aagtggacga ttcgcctgat acgcaagagg
     4621 aaagccaggc cttgctgctg cgtgccaccc gcatggataa cagcgacacc ctgtgggatc
     4681 acttgttcga agatgaaagc cagcaaactg cattaccctc tgcactggcg cactattttg
     4741 cccaactgcg gggcgactcc cccggagatg ctctcaatcg tctgcgcgaa gcctttatgg
     4801 cccgctggat tggatgggcg atgcagcaaa ataatggcga cgtgttagtt gtctgcggtg
     4861 gctggcacgc tccggtactg gcaaaaatgt ggcgcgaatg cccgcaggaa attaacacgc
     4921 cagaattgcc gtcgctggca gatgccgtta caggttgtta tctcaccccc tacagtgaaa
     4981 agcgccttga tgtgctggca ggataccttt ccggaatgcc tgccccggtc tggcaaaact
     5041 ggtgctggca gtggggctta cagcaggcgg gagaacaact gctaaaaacg gttctcaccc
     5101 gtttgcgcca gcacaagttg cctgcttcga ccgcggatat ggctgccgct catctgcatg
     5161 cgatggcact ggcacagttg cgcggtcata cactaccgtt acgcactgac tggctggatg
     5221 ccatagcagg atcgctgatt aaagaagccc tgaatgcgcc gttgccgtgg agctatcgcg
     5281 gcgttattca tcccgacacc gatccgattc tgctaacgtt gatagacaca ttagcgggtg
     5341 acggattcgg taaacttgcc ccttctacgc cacaacctcc tctgccaaaa gatgtcacct
     5401 gcgaactgga acgtaccgca atctcccttc cggcggagct taccttaaat cgctttaacc
     5461 ccaatggact agcgcaaagt caggtgttac atcggctggc aatactggag atcccaggga
     5521 ttgtgcgcca gcagggaagc acactgacac ttgcaggcaa cggtgaagaa cgctggaaat
     5581 taacccgccc gcttagccag catgcggcat tgattgaggc cgcctgcttt ggtgcaacac
     5641 tccaggaagc cgcacgccat aaattagaag ccgatatgct ggacgcgggt ggaatcggca
     5701 gtatcaccac atgtcttagc caggcggcgt tagcgggtct ggcgtccttc agtcaacaat
     5761 tactggagca actcacatta ttaatcgccc aggaaaatca atttgccgaa atgggccagg
     5821 cgctggaagt gctatatgcc ttatggcggc tggatgaaat tagcggtatg caaggcgcgc
     5881 agatattaca aacgacgtta tgcgcggcta tcgatcgcac gctgtggcta tgtgaatcta
     5941 acggcagacc agatgaaaag gagtttcacg ctcacctgca tagctggcaa gcgttgtgtc
     6001 atatcctgcg cgatctacat agcggtgtgc aactccccgg catttctctt tctgcggcgg
     6061 tagccttact ggagcgacgc agtcaggcaa ttcatgcccc ggcgctggat cgcggcgcgg
     6121 ctcatggcgc actgatgcgt ctggaacatc ctaacgccag tgccgaagcg gcgctgacga
     6181 tgctggcgca gttatccccg gcacaatccg gcgaagcgct gcacggtttg ctggcgctgg
     6241 cccgccatca actggcctgt cagccgacat ttatcgccgg tttcagcagt catttaaatc
     6301 aactaagtga tgccgatttt atcaatgccc tgcccgattt acgcgcggcg atggcctggc
     6361 taccgccacg agaacgcgga acgctggcgc atcaggtgct tgagcattat caactggcgc
     6421 aacttcccgt ttcggcactg caaatgccgt tgcattgtcc accgcaagcc attgcacatc
     6481 atcaacaact cgaacagcag gcactggcat cgctgcaaca ctggggagtt ttccatgtct
     6541 gaactgaacg atcttctgac cacccgtgag ctacaacgct ggcgattaat tcttggcgaa
     6601 gcggcagaaa cgacgctttg tgggctggat gacaacgccc ggcagataga tcacgcgctg
     6661 gagtggctgt atgggcgcga tcctgaacgg ctccagcgtg gtgaacgctc tggtggatta
     6721 ggtggctcaa atctcaccac tcctgagtgg atcaacagta tccacacgct gtttccgcaa
     6781 caggtgattg agcggctgga aagcgatgcc gtactgcgct acggcattga agatgtggtg
     6841 acaaatctcg acgtgctgga acgtatgcag ccttcggaaa gcctgctacg cgccgttttg
     6901 cacaccaaac atctgatgaa tcccgaagta ctggctgccg cccgccagat agtgcgccag
     6961 gttgttgaag aaattatggc gcgactggca aaggaagttc gtcaggcttt ttctggtgtc
     7021 cgcgatcgcc gtcgccgttc atttattcca ctggcgcgaa actttgattt caaaagtact
     7081 ctgcgcgcca acctgcaaca ctggcacccg caacacggca agttgtatat cgaatccccc
     7141 cgctttaaca gccgcattaa acgccaaagc gaacaatggc aactggtctt actggttgat
     7201 caaagcggat cgatggtcga ttcggtgatc cactctgcgg tgatggcggc ctgtttgtgg
     7261 cagttacccg gcattcgtac ccatctggtg gcgtttgaca caagcgtcgt tgatctcacg
     7321 gcagacgttg ccgatccggt agagttatta atgaaagtac agttgggcgg cgggaccaat
     7381 atcgccagtg ccgtggagta tggtcggcaa cttattgaac aaccagcgaa aagcgtcatt
     7441 atcctcgtga gcgattttta cgaagggggt tcatcatcat tactgacgca tcaggtgaaa
     7501 aagtgtgtcc agagctgcat caaagtgctg ggactggcag cgctcgatag cacagcaaca
     7561 ccttgctatg accacgatac ggcccaggcg ctggtaaatg tcggcgcaca aatagccgcc
     7621 atgacaccgg gcgagctggc atcatggctt gcggagaatc ttcagtcatg aattcactac
     7681 gtccggaatt attagaactg acaccgcagg ccctgacggc gttaagcaat gccggttttg
     7741 ttaagcgcag tcttaaggaa ctggaaaatg gcaacgtccc ggaaatcagc catgagaaca
     7801 gcgctttaat cgccaccttc agtgacggtg tccgtaccca gctggcgaac agccaggcac
     7861 tgaaagaggc tcagtgcagc tgcggggcca gcggtatgtg ccgtcatcgc gtgatgcttg
     7921 tgttaagtta tcaacgactt tgtactaccg cccagcctac ggaaaaagaa gaagcgtggg
     7981 atccggcaat ctggctggaa gaactggcta ccctgcccga tgccacccgc aaacgcgcac
     8041 aggcgttggt cgctaaaggc atcaccattg agttgttctg tacgccgggc gaaattccct
     8101 ctgcccgctt accgatgagc gatgtgcgtt tttattcccg cagcagtatt cgtttcgccc
     8161 gttgtgattg tattgaaggc acactttgcg aacatgtcgt actggctgtg caggccttcg
     8221 tcgaggccaa aactcagcaa gcggaattta ctcatttaat ctggcagatg cgcagcgaac
     8281 acgtcacatc atctgacgat ccgtttgcca acgacgaggg taacgcgtgt cgtcaatatg
     8341 ttcagcaatt aagccaggca ttatggctgg gcggcatcag ccagccgctt attcactacg
     8401 aggccgcttt cagtcgcgcg cagcaggcgg cggaacgctg caactggcgc tgggtgagtg
     8461 aatcactacg acagctacgg gcaagcgttg atgccttcca cactcgcgcc agccactatc
     8521 atgccggaga atgcttacgt cagcttgcgg cattaaacag tcgattaaat tgcgcacaag
     8581 agatggcccg gcgcgacagt gttggtgaag ttcctcctgt gccgtggcgc acggtcgttg
     8641 gctctggcat tgccggagaa gcaaagcttg atcatctgcg gctggtgtct ttaggtatgc
     8701 gttgctggca ggatattgag cattatggtt tacgcatctg gtttaccgat cccgacaccg
     8761 gcagtatttt gcacctttcg cgcagttggc cgcgaagtga acaggaaaac tcaccggcag
     8821 ctacgcgtcg gctgtttagt tttcaggctg gcgcactggc gggtgggcaa attgtttcac
     8881 aagcagcaaa acgcagtgcc gatggcgagc tgctgttagc tacccgcaac cgcttaagca
     8941 gcgttgtgcc gctgtcgcct gatgcctggc aaatgttgag cgcgccgtta cgccagccgg
     9001 gcattgtggc tttgcgggaa tatttacgcc agcgtccccc cgcctgcata cggcctctta
     9061 atcaggtcga taacttattt attctgccgg tcgctgagtg tatttcgctc ggttgggata
     9121 gcagccgcca gacgctggat gcgcaggtaa tcagcggcga aggtgaagat aatctcctga
     9181 cgttatccct tccggcatct gcctgttctc cttttgccgt tgaacgcatg gcggcgcttt
     9241 tgcaacaaac agacgacccc gtgagtctgg tttctggctt tgtcagtttt gttgatgggc
     9301 aattgacact ggaaccacgg gtgatgatga caaaaacccg cgcctgggcg ctggacgcgg
     9361 aaactgcgcc tgtggcaccg ctaccttctg ccagcgtttt gcctgtgccg tctaccgctc
     9421 atcagttgct gatgcgctgc caggcgttac ttattcaact gctccataac ggctggcgct
     9481 atcaggaaca gagcgctatt agtcaggcag agttgctggc gaatgacctc tccgcggtcg
     9541 gtttttatcg gctggcacat gtgttgggac aatttcgtaa tacagaaagc gaggcacggg
     9601 tagaagcaat gaataacggt gttttacttt gcgaacaatt attccccatg cttcagcaac
     9661 aaggatgaaa tagtgctttt tactaagagt tctactccag ttccggactg ctcacgtcac
     9721 ggtattaggc atattctata tagcccctgg tgagagtcac cagtttcttg attaaataaa
     9781 atggagtttt acatgaaggc tttcaataag ctgttttccc tcgttgttgc atctgttctg
     9841 gttttctctc ttgctggctg cggtgacaaa gaagaatcga agaaattcag cgccaatctg
     9901 aacggcactg aaattgccat tacctatgtc tacaaaggtg acaaggtgct taagcaatct
     9961 tctgaaacca aaattcaatt tgcttctatt ggtgcaacca ccaaagaaga cgctgccaag
    10021 acacttgagc cgttaagcgc caaatacaaa aacatcgcgg gtgttgaaga aaaattaacc
    10081 tatacagata cctacgcgca ggaaaacgtg actatcgata tggaaaaagt ggattttaaa
    10141 gccctgcagg gtatttcagg aatcaacgtt tctgctgaag atgccaaaaa aggtatcact
    10201 atggcgcaaa tggaactggt gatgaaagcc gctggtttta aagaagtgaa ataatctgtc
    10261 ggcggccatg tttcgcatgg ccgccatacc cgtcttagct tttcttcaca tgctggcgcg
    10321 ctgccagtcc acgcagaaaa taacgtaaaa attgatcgcc gcattcgcgg aagtttttat
    10381 gatccggtgc gcgcatcatc gctgtaattt ccggcatcga aacgcggaac tgctgttcgg
    10441 tgaggatagc cagaatgtca tcggttttca gcgaaaacgc gatgcgtaat tttttcagca
    10501 cgatgttgtt attaatgcga cgttccggct ccagtgccgg agcagactca tccttgccgc
    10561 gtttttcata aatcaggccg ttgaggaatg acgacaaaac aatgtccgga caacgctgaa
    10621 aaccctcttc gtcttcttta cgtagccaga cggcgatctg ttccgcggtg gcttcgacat
    10681 tacccagcgc cagaatacgc accaggtcat tattattggc tttcaaaatg tagcgcacgc
    10741 tgcgcagaat atcgttactt agcatgaggc cttcaggtgt tgatgaggca aaaagccatt
    10801 ttagcagtct tttacaggcc aatcgcctct tttaagcttt tcagataacg gcggctgacc
    10861 ggcacggtta agccattacg caaaatcaac tcggcctggc cgttatcttc cagacgaatc
    10921 tcctgtaaat gcgcgagatt aaccagatac tgacgatggc agcgcagtag tggtgtacga
    10981 ctttccaggg tacgtaatgt caattcggta aagccctctt tcccttcgtg gctggtaacg
    11041 tagacaccgc tcatccgact gctgacaaat gccacatctt tcatttgcag caaataaatc
    11101 cgactatgcc ccgtacaagg gataaatttc agcgcctgtt gattttccgg taacagcgaa
    11161 acatcctgct tgctgcgctc ctgacgcaat cgcgccagcg ttttctccag tcgcgcttca
    11221 tcaattggct tcagcagata atcaaaagca tgttcttcaa aggctttaat agcgtattcg
    11281 tcaaacgcgg tgagaaaaac aatatacgga cgatgttccg gatcgagcat ccccaccatt
    11341 tccagaccac tgatgcgcgg catctggata tcgagaaaca gcacatccgg gcgcagttta
    11401 tgcaccgcgc cgatcccttc cacggcgttt gaacactctc caacgatttc aatatcgctc
    11461 tgctcctgca aaaatacgcg caggttctcc cgtgctaacg gttcatcatc gacaattaag
    11521 actttaatca tgcctcgtcc ctccatggta gtcgtaacgt tattcgggtg taactatcag
    11581 gctcacaggc gacgcttatc ccatagtcat cgccaaaccg ttcacgtaaa cgcttatcca
    11641 ccagattcat ccccagccca ctggcattgg ttaccggttg atacaaaccg gcattgtctt
    11701 cgatctccag catcaaatgt tgcccctcac gtcgggcgct gattgccact cgccctgtat
    11761 ccagcagttg tgatgtccca tgtttaatgg cgttttccac tatcggttgc agggtaaacg
    11821 cgggcaattg ctgctgggat aattcttgcg gaatagcaat gttgacctgc aaccgcgact
    11881 ggaagcgcgc cttttcaatt tgcagataag cgttcacatg ttcaatttcg tcggcgagag
    11941 taacaaactc cgaaggccgc tttaagtttt tgcggaaaaa agtggaaaga tactgcacca
    12001 gctggctggc ctgttcgctg tcgcggcgga tcaccgcttt aatggtgtta agcgcattaa
    12061 acaaaaaatg aggattcacc tgggcgtgaa gcagtttgat ttctgactgg gtgagcatcg
    12121 ctttttgccg ctcatattgc ccggcaagaa tctgcgccga aagcagttgc gcaatcccct
    12181 cgcccagcgt gcggtttatt gaactgaata aacggttttt ggcttcatac aatttgatgg
    12241 tgcccatcac gcgctgattt tcgccacgca acggaattac cagcgtcgac cccagtttgc
    12301 attgcggatg caaagagcaa cgataaggta cttcgttgcc atcagcgtag accacttcac
    12361 cggtttcaat cgcttttaag gtgtaagtcg aagaaatcgg tttgccgggt aaatggtggt
    12421 cgtcaccaat tccggtaaag gccagcaatt tctctcgatc ggtaatcgcg actgcaccaa
    12481 tatccagctc ctgatacagc acctgagcca ctttcatgct gttcacttcg ttaaacccct
    12541 gtcgcaaaat gccttccgtc gaggctgcca ctttcagcgc agtggcagaa aaagcagaag
    12601 tgtatttttc aaacatcgcg cgtttatcga gcaatatacg cataaacagc gccgcgccga
    12661 cggtattggt gaccatcatt ggtgcagcaa tattactcac cagacgcacc gcatcttcat
    12721 aaggtcgggc gatcgcaagg atgatcagca tttgcaccat ttcagcgacg aacgtgacgg
    12781 caccggcggt aatggggtta aagactttat cagtgcgccc gcgacggatc aggatgctgt
    12841 gtaccaggcc gccgagcaat ccttcaacga tggttgagat catgcaactt agcgcggtca
    12901 tgccccccat cgaatatcga tgtaagccgc cggtcagacc aaccagccca ccgacgaccg
    12961 gaccgccgag taagccgccc attaccgcgc ctatcgcacg ggtattggca atagaatcgt
    13021 caatgtgcaa cccaaaccag gtgcccatga tgcagaagat ggaaaagacg atgtagcaga
    13081 gaaatttatg cggcagacga accgtgacct gcattaacgg tatgaataat ggcgttttac
    13141 tcattaacca cgcaatgact aaaaaaacgc acatctgctg aagcagcagc aacaccagat
    13201 taaaatcgta catacccgca aaccacactt ccctttaaaa cgcgtaacat acattgcctg
    13261 cgtttaactt tctttgaact cttgcagaaa aatgagaatt cgtgagtacg atcactcaaa
    13321 atcgcctggc aaaaataaaa tcaccctata gatgcacaaa aaacgggcaa aactacctgg
    13381 ttcgcaaaac tgcgtctaaa gttaaaccgg gacctcgcga gcaagggtga gacgatggcg
    13441 ctttacacaa ttggtgaagt ggcgttgctt tgtgatatta accctgtcac gttacgcgcg
    13501 tggcagaggc gttacgggtt gctgaaaccg caacggacag acggcggtca tcgactgttc
    13561 aacgatgccg atattgaccg tatccgcgag atcaaacgct ggatcgacaa cggcgtgcag
    13621 gtcagcaaag ttaaaatgct gctcagtaat gaaaatgttg atgtgcagaa cggctggcgc
    13681 gatcagcaag aaacattact gacctacctg caaagcggca atctgcatag cctgcgaacg
    13741 tggatcaaag agcgcggtca ggattacccc gcccagacac tcaccacaca tctgtttatt
    13801 cctctgcgcc gacggcttca gtgccaacaa ccgactctcc aggcgctgct ggcgatcctc
    13861 gacggcgtac tgatcaacta catcgccatt tgtctggctt cggcacgtaa aaaacagggt
    13921 aaagatgcgc tggtggttgg ctggaatatt caggatacca cccgtctgtg gctggagggc
    13981 tggattgcca gtcaacaagg atggcgcatt gatgtcctcg cccactcgct caatcaacta
    14041 cgccctgaac tattcgaagg ccgtacattg ctggtgtggt gcggtgaaaa tcgaacctcc
    14101 gcccaacagc agcaactcac cagttggcaa gaacaaggcc atgatatttt cccactcggc
    14161 atttaatgat tcgttaacaa atgcgcttta ctgtacaatc ctttcgttaa cataaggagt
    14221 gcaatatgcg catagctaaa attggggtca tcgccctgtt cctgtttatg gcgttaggcg
    14281 gaattggtgg cgtcatgctc gcaggttata cctttatttt gcgtgctggc taagcgcctg
    14341 caccagcctt tcaaacaggc ggtctgcgat gatcgccgcc agtgccacca gtaacgcccc
    14401 ctggatcaca tacgcggtat taaatccgct aagcccgatg atgatgggcg tacccagcgt
    14461 gctggcccct accgttgagg cgatcgtcgc cgtaccaatg ttgataatca ccgaagttcg
    14521 cacgcccgcc agaatcaccg gagccgccag cggtagctcg accttacgca gtcgctgacc
    14581 acgactcatt cccatacctt tcgcaacttc tgtcacgctg gcatcaatcg ctcccagccc
    14641 ggcaagtgtc gcctgcagga cgggcagcac accgtaaagg atcaaggcga taatcgctgg
    14701 ttgcagacca aagccgatca ccggaacggc aatcgccagc actgcgacag gcggaaaagt
    14761 ctgtccaacg gcggcaatag tttccaccag tgggcgaaat tccgcgcccc acgggcgagt
    14821 gacagcaatt ccggcaccag tgccaatgat caccgcaaac aaactcgaaa ttcccaccag
    14881 ccagaaatga gccagtgcca gagctgcaaa actttcttgc tgataaacgg gtcgtggcag
    14941 ttgtgggaac aaggcagcaa acagcggctg gctgtaaggc agccagaaaa tcagtgccac
    15001 aaacaaagca atgagccaga acagcggatc gcgcaacatc ttcatacgct tacgcctcct
    15061 ccagcagatc ctgaaaatgc agcgtgccgc aaggctggcc ctgcgtgttc accaccggca
    15121 gcacctcgca tccccgcgcg acaaacagag agagcgcatc gcgtagcgtc atctcttctg
    15181 ccagtgcctc accttctgca cgttcttcgc gacgcacgta atccgccaca ctacgtaacg
    15241 aaagcaggcg cacacccagt tcactacgtc caaaaaactg gcgaacaaaa tcattcgccg
    15301 gacgagtcag catcgtcagc ggattgccct gctgcaccac ttcaccgtga tccatcaata
    15361 ccagatgttc tgccagccgt agcgcctcat caatatcatg agtgaccagc acaatggtac
    15421 gccccagcaa acggtgaatg cgcgtcatct cttgttgcaa cgcgccgcgc gttaccgggt
    15481 ccagtgcgcc aaaaggttca tccatcagta agacttgcgg atcggcagcc agtgcgcgcg
    15541 ccactcccac acgttgctgc tgaccaccgg aaagctgatg cggataacgc tcacgcaaat
    15601 ttgactccag ccccagtagc gccattaatt cgtcgatacg atcgtcaatc cgcgcccgcg
    15661 accatttttg taattgcggc acggtagcga tgttttgcgc cacactccag tgggggaaca
    15721 ggccaataga ttgaatggca tagcccatcc ggcggcgcaa ctccagtact ggcagcgagc
    15781 gaatttcttc tccggcaaag cggatcacgc cgctgtcatg ctccaccagg cggttaatca
    15841 ttttcagggt ggtggatttg ccggagccag atgtgccaat cagcaccgaa aaactccctt
    15901 cctgaaaatt gagattgaga tcgttaacgg ctttttgtgc gccgaacagt ttgctgacat
    15961 ggctaaattc aatcattacg tttcaccttc agcagtgcga taagcaaatc gaacagcgcg
    16021 tcggtaagca ccgccagaac aattaccggg atcaccccca gcaacactaa atcaatggca
    16081 ctgcttagca gcccctggaa aaccagcgca ccaaaaccgc ctgcgccgat taatgccgca
    16141 atcaccgcca tgccgacggt ttgcaccatc accacccgca ggctgcgcag aaataccggt
    16201 aacgccagcg gtaactgaac atgctggaat cgctgcgccc cgctcatgcc catcgctctg
    16261 gcgctctcca gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc
    16321 ggcagcaagg catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg
    16381 ccgagcttcc ccagccacgg aaaggccgta accagcgcgg caagcggcgc aatcaacagg
    16441 ccaaagagcg ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc
    16501 tgccgcgcag tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc
    16561 agcaccgcac caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga
    16621 cggttggcgt attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac
    16681 agcggaataa tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt
    16741 cggcggatgg catcgctaca ggccagcaat gccagcgccg cagccagcca gaaaccactg
    16801 ccgaggctgg tacgcgccag cgcactgcca ttttgcgcca gttgggtcgc cgcctttccc
    16861 gcgccccaca ccaacaatac gaagacgaat tgcgccagaa tgagtgcaca aatgctgcct
    16921 tttttagcgg ggacaaaaca ggctgtcagc caggcgcaac caacgcccac cagcatccag
    16981 atcgtttgcg gccacagttg ccagagatga cgcccttcac ccgagactaa acgattaggc
    17041 gcgtagctga taaacggcag cgctgctgcg attgccgtca gcaacagcag cagcgccaga
    17101 acaggattaa tacgtagata agtcacggga aattacttcg cccacccttt ttgtttcagg
    17161 tagtcggcag ccactttttt ggcatccagt ccttccactg caatgctggc attcagttgc
    17221 tgcaatgttt ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga
    17281 tactccttca acaccgattc acgcaccact ggtgcaggcg catagatagg ttgcacgcct
    17341 tgcggatcgc ttaaggtttg cagccccagc gccgctaccg ggccgtcagt gccgtaagcc
    17401 attgcagcat taacgccaga ggtttgctgg gcagcggctt tgatcgtcac cgccgtgtcg
    17461 ccaccagcca gtgacagcaa ctgatcctga ccgagcttaa agccataggc tttttcaaac
    17521 gcgggtaacg catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg
    17581 ccctcttgca gataacgact caggtcggca agcgaagtga gtttattttt ctctgccaca
    17641 tcctgacgca cggcgatagt ccaggtgtta tttgcaggcg cgggcgtcag ccagattaac
    17701 ttgttgtgct ccgaatcgag ctttttgact ttctcgtaac cttgctgcgc gtttttccac
    17761 gctgcatcgt tttcatcttt aaagaagaaa gcgccattgc cggtatattc cggatagata
    17821 tccagttcac cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta
    17881 ttcaccgtcg gtacgccgtg gctttccagc acctgcaaaa tgatattgcc gagtagcgca
    17941 ccttcggtat cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc
    18001 acggctgcca acataaccag tgaacctgcc cagagcttta agagtggcat gatgctttcc
    18061 tcattctttt actgttgttt tcagcgaatt aagagaaaag catagttgat aatggcgggg
    18121 ttagcttgag cggaaggatt cagttgcaga atcagataaa tacttaagag gcatattcgg
    18181 tccggcgttt cctgccggat gcggcgcgag cgccttatcc ggcctacaaa gggcgcaaac
    18241 gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa
    18301 tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc
    18361 cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc gcccggtttc
    18421 agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga agccgtcaca
    18481 tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt cgtcacctgc
    18541 acgctggcag tcactttgcc gtcacgcttc atggtcggcg cagaaagttt cacatcagag
    18601 acggtgaaag tggtgtagct cagcccatag ccgaacggat acaacgcccc gttagcttca
    18661 tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacgggcg accggtattc
    18721 agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat tggcagcttg
    18781 ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc ttcagtcccc
    18841 gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga
    18901 cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc cgcaatcaag
    18961 tcacgttggc tttgcggaat agtgatatcg gtccggctgg aggcttcgtg cgccatcccc
    19021 tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg caccgcttca
    19081 tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata ctgattcagg
    19141 aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg
    19201 ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc ggcaacaccg
    19261 gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc aaccaccgca
    19321 atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa caccaggctt
    19381 tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt ggtatccacc
    19441 gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaatcc catatcatat
    19501 ttaacgttca gtacatggcg ggcagcatcg tccagctctt ccatcgtcac tttgccggat
    19561 ttgatcaacc caggcagata cttcgagtaa tactcgtcgc tcatactcat gttgattccg
    19621 gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg tttaatcagc
    19681 tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga
    19741 acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga gttcagcgcc
    19801 accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat ataatcatta
    19861 aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg
    19921 tatgcggcaa agtgtttgac gctggtcatc accgagtagc gatctgccgg gcttttaccc
    19981 tgcatcgctt ccaccatggt tttacccatt gttgaggtga gatacgtatc ttcgccaaaa
    20041 ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc
    20101 atattcaggc catcatctgc cgcttcataa gcagagacac gtccgaccgt tttcactgca
    20161 tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc
    20221 agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc
    20281 tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac ctgaccgtct
    20341 ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat taagcgcagc
    20401 tgaccaattt tctcatcaac tgtcattttc ttaagcagtt cggtgacgaa cgcatcccgc
    20461 gcttcgggcg ttaatggatg gttgccgaat aaatcatccg ccagtgctgg ctgcagggcc
    20521 agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct tcctctgttg
    20581 cccgacgagg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga
    20641 gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat
    20701 acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg aaaatgtctt
    20761 ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt
    20821 cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc cgttctggtc
    20881 agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga
    20941 aagcctgtgt caccgccgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg
    21001 aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc agcaccctgc
    21061 gtctcgacaa gctgcacgtt cttggcaagg gcgaacaggt gctggcctat ccgggcacca
    21121 cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcac tcggtgattg
    21181 gatcgtcgtg tataggcgca tcggttatcg gcggtatttg taacaactcc ggcggctcgc
    21241 tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata aatgaagacg
    21301 gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc
    21361 ttagcaagct ggatgatgat cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg
    21421 cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg cccgcacgtt
    21481 ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag ctggccgtct
    21541 ttgcggtacg tctagatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca
    21601 ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac ttcgaaaatc
    21661 tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca
    21721 aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc
    21781 tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttactg
    21841 accgtgcgat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa
    21901 actggcgcga taaatacgag catcatctgc tgttaaaaat ggcgggcgat ggcgtgggcg
    21961 aagccaaatc gtggttggtg gattatttca aacaggccga aggcgatttc tttgtctgta
    22021 cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc
    22081 gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcgttggat atcgctctgc
    22141 ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc cagctggtgc
    22201 acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac atagtgaaaa
    22261 aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag cagcgcggcg
    22321 cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag acgttgcaga
    22381 agttctatcg cgagaacgat ccgaccaaca gcatgaatcc ggggatcggt aaaaccagta
    22441 aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat cgccatccga
    22501 attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt
    22561 tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa tccaggtacg
    22621 caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca ctaccagcgc
    22681 caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg cattggtaaa
    22741 gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat tagtattgcg
    22801 gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg tagtacttaa
    22861 ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt cacgggcagt
    22921 tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt tcattccgag
    22981 cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg gataatggtg
    23041 cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca gcatatcttt
    23101 acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg
    23161 gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca caaccatcgc
    23221 ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt tcgaatagat
    23281 cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt ccggttgtga
    23341 agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gccaaaggca cagccagcat
    23401 cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc agatagtggt
    23461 tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc gacgccatca
    23521 taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag gaaatgctgc attgctgaca
    23581 tttacgccag caatgcaacg tcaaagaact ttctagaaca ggcgataccc gtagccccat
    23641 agtataacgg ttagggcgag cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa
    23701 ctagagaagc taaggccttc ctctttgttg atattcagga agctcggaat accaaggtaa
    23761 agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa ccagaccagc
    23821 ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc cgcgaagacc
    23881 atgcagtgcg caagtgacgg acgctgcgga taattacgcg ccatccacca gatgacccgc
    23941 cccattaccg caaccccagc cagcataacg ccataaaaca ggacagccag cgccagtccg
    24001 gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg
    24061 ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg
    24121 tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg atgggaaaac
    24181 agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct gacagttcaa
    24241 gtataagtca gcttgtgatt attttttgtt cgcccatgta attttcactg tctgatgaac
    24301 gtcctttttc agcgtgaatg attcacaggg tgtatgctta ctggcaacca aagggagaca
    24361 gactggccta tggatctcaa tacacttatc tcacaatatg gttatgccgc gctggtgatc
    24421 ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gcgttgcggc gcatcagggg
    24481 ctattaaagt tcccgctggt ggtactttct gtggcgcttg gcggcatgat tggcgaccag
    24541 gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt ctcgaaacat
    24601 caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct gtttgtcatt
    24661 ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg tgccagccag
    24721 ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg ggcgttgatt
    24781 tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgac
    24841 cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt gggcgtgcgc
    24901 tggtggctga aacggcgcgg gaagaaaaag ccggatcatc aggcgtaaaa ccattgccct
    24961 gggtaaggcg ttcgcgccgc atccgacatc cggtgcacca tgcctgatgc gacgcttgtg
    25021 cgtcttatca tgcctaccaa aattttaccc attgaaataa ccacgcacct cactgcgcta
    25081 ttctgggttg aactgcggat tcgccaacat aaagccgcca tccactatca acgactgccc
    25141 ggtggtgtaa tttgcgccct ctgagcacag ccacgctacc aggctggcaa tctcatgcgt
    25201 tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt cgctgtcatc
    25261 catgccattc attggcgtgg cgatcgcccc aggcgcgact gcgttcacca aaatcttatg
    25321 cctgaccagc tccagcgcca tcgttttggt taacccgccg agcgcatgtt tagcggctgt
    25381 gtaagcgctg gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg
    25441 accgccctgc ccttgtttca ccatctggcg ggccgcaatt tgtgagcata aaaatgcacc
    25501 atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcgc
    25561 tttggtcatt gcgcccgcat tattcaccag cacatcaata cgccccagcc gttgaatgag
    25621 tttctccagc gcctgcgcac cttctggcag tttaccgaga tccagctgca cgatctccgc
    25681 acgtacgccg tggctaacta cctcacgcgc ggtatctttt gccccttctt catctgagtg
    25741 ccaggtaatg ccaatatcaa acccctgctg cgccagtaat aacgcgcact ctttgccgat
    25801 ccccgaatcg gaggcggtaa taatcgcaac ctgtgccatc gagttctcca cttaacgctg
    25861 aataaacgtt aagtatagaa ggcgcatatc atcagcgttt gtaacccccg cccaacgcac
    25921 cagtgagttg aatggaagca tccagccact gcccttgcaa taacaggcca ttggcccgct
    25981 cacgcagcgc ggggattctg gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa
    26041 agcgcgcctg cgcaagacct accacacgca aggcatcgcg ctcaatttgc gcctgatgct
    26101 ggtttttctc cgcaagtgtc tgaacctgac tggctgcccg cgccacgtca ttcaccgctt
    26161 caaccaccgc tttgttgtag ctggcgatag acaagttgct ttcggctttt gcgatatcga
    26221 gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca
    26281 tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg
    26341 ccatcaggtt gatgtcagga taaaatgccg cttttgccgc atcaatggtg cttagcgatg
    26401 actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag gagtacccca
    26461 gttcatcagg aagctggctt gccactttcg gcaacgcgac cggtttaagc ttcaatgact
    26521 ttgtctggtt atttgtaagt gcgcttaacc gtgcctcaat aattttcatt ttccccgcga
    26581 catcgttgag ctgctgccgg gttttgctgg cattaatatc ggtttccaca ccttcaactg
    26641 aagaagtaat cccgttctga tatagctggc gatcggtcgc gataatggtg ttctgctctt
    26701 tttctatttg ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc
    26761 gggctacgct gccagccagc aattggcggg tttgctcgcg ttccgccgcc cgtgctttaa
    26821 ccgtacccag gcgggcagta acctccgccc gattctttcc ccagatatcg agatgccagc
    26881 ccgccgttaa gccaaaagtt ccgttggtgt accacggacc ggtcgtacct gcggccggat
    26941 cgttcagagc aaacggcccc attaagcctt ctgccgacat tttttgccgc tccatatccg
    27001 cagaaaagtc gatctgcgga ccatcctgag tagcaattgc cttcgcctgg gcttcagcta
    27061 gctgaatgcg ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta
    27121 aggaagtgag ttgattatcg tgatactcca gccaccattg gctgtctggc caaccatttt
    27181 tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcagg cgtttgctgg cttaacgcct
    27241 ggcgggtttc atgcataggt gcacacccgg ccagcatcag taacagcgga aaacaggcga
    27301 tggcgggata aaaggaatca cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg
    27361 agattaccgt gtgttgcgat atatttttta gtttcgcgtg gcaatacatc agtagcaata
    27421 aaacgacata tccagaaaaa tatacactaa gtgaatgata tcttccgatt tatcttaatc
    27481 gtttatggat aacggcaaag ggcttcgttt tttcctatac ttattcagca ctcacaaata
    27541 aaggaacgcc aatgaaaatt atactctggg ctgtattgat tattttcctg attgggctac
    27601 tggtggtgac tggcgtattt aagatgatat tttaaaatta attaatgtca tcaggtccga
    27661 aaataacgag aatatttcag tctctcatcc tgttgcgctc ctgtcatgtg cattgcttca
    27721 tataatcact ggcgcaagga gcgcgcaggg ggcggccaat cgccgccgcc ccctgcaccc
    27781 ccgggctctg gcgaacaaaa tcgccgctgc gcggtgccct cggcttatcc cttacggcta
    27841 ccgggtcggg cgcgaggtaa catccctgta aaacgcgccc tcagcccaca tccatgtggg
    27901 ctgccccggc cttcagggaa cgcctcggca attttgacgc caccaaacaa ccgtgcggcc
    27961 tattgataaa gagctaacac attgtcaaaa aacatcacta tggtttttta gagtttctcg
    28021 atatcaattg cctgaatagc ccttgcaata tcaggggaat tattcaacac ccgaacatgc
    28081 tgaaataatc ccgttgcttc atcgtattct ttacgcaaat aactcaacca ctgtttaatc
    28141 cgcgcaacgt gatataaccc ggtatcgccc tgcttttcca gacgggtata tttttgcagc
    28201 aaagcaacca cctccggcca cggcattcgc ggttcgttat attttactac ccggctcagg
    28261 ttgggaatat tgagcgcccc gcgaccaatc atcactgcgt cgcagccgct gatcgcaatg
    28321 cattgttgcg cgctctgcca gtcccagatt tcgccgttgg caatcaccgg aatattcagc
    28381 cgctggcgaa tctcgccaat cgcctgccag tcaatatgct ccgcgcggta accctgctct
    28441 tttgtccgtc catgcaccac cagctccgta gcaccagcct gttgaaccgc atcggcgatt
    28501 tcaaatttct tctcaccgct gtcccagccc agacgcactt tcacgctgac gggcaaatgc
    28561 gccggtacag cttcacgcat cgcttttgca ccctgataga tgagttcagg atctttgagt
    28621 aacgtcgccc caccaccgct accgttgacc gttttcgacg ggcagccgca attgagatcc
    28681 acgccccagg aacctaattc caccgcacgg gcggcgttct ctgccagcca ttgtgggaac
    28741 tgacccaaca actgcacgcg caccagcgta ccggatggtg tccggctggc gttttgtagc
    28801 tcagggcaaa tgcgatgaaa aacttttacc ggcagcagtt gatccaccac gcggacaaac
    28861 tcggtgatgc acagatcgta gtcgttaact tcggtcagca attcacgcac cagagagtca
    28921 agcactccct ccatcggtgc cagtaacaca cgcatatcat cacccgcaaa ataatgaggc
    28981 gctatgttag cgcctatggt cagcggatta aaggtctaca aagagggatt gctgcaaact
    29041 gcaatccctc tctatgcttg atgtatgcct gagttcgttg cgcattgcgt aatgccgggg
    29101 agcgaaaaaa taagcgctga tttcttaatg tgatcggtag cacgttttac gaattaattg
    29161 tatgatgaat ccatctcatc tggggtgttg attatgagta agacactgaa cattatctgg
    29221 caatatttac gcgctttcgt cctgatttat gcctgcctgt atgcaggcat tttcattgct
    29281 tccctgctac cggtaaccat tccgggcagc atcatcggga tgctgatcct gtttgtcctg
    29341 ctggccttgc aaattcttcc ggcgaaatgg gtcaatccgg ggtgctacgt actgattcgc
    29401 tatatggcgc tattgtttgt gccgattggc gtaggcgtca tgcaatattt tgatttgctc
    29461 cgcgcgcagt ttggcccggt agtggtttcc tgtgctgtca gtacgctggt ggtttttctg
    29521 gtggtgagct ggagttcaca actggtacac ggtgaacgta aagtcgtagg tcagaaagga
    29581 tcagaagaat gatggcgaat atctggtggt cattaccgct gacgttgatt gtcttttttg
    29641 ccgcccgcaa actggcggca cggtataagt ttcccctgct taacccgttg ctggtagcaa
    29701 tggtggtgat cattcctttt ttaatgctga ccggcatctc ttacgacagc tactttaaag
    29761 gtagcgaagt gctaaacgat ctgctgcaac cggcggtcgt cgcgctggcc tatcctttat
    29821 atgagcagct acaccagatc cgcgcgcgct ggaaatcgat catcaccatc tgttttatcg
    29881 gcagcgtggt tgcgatggtg acggggactt ccgtggcatt gttgatgggc gcttcaccgg
    29941 aaatcgccgc gtcaatcctg ccgaaatcag tcaccacgcc tattgcaatg gcggttggcg
    30001 gcagtattgg cggtattccg gcaatcagcg cagtttgcgt gattttcgtc ggcatcctcg
    30061 gcgcggtatt tggccataca ttgcttaatg cgatgcgtat tcgtaccaaa gctgcgcgcg
    30121 gtctggcaat ggggactgcc tcgcacgccc tcggtacggc gcgctgcgcc gagctggatt
    30181 atcaggaagg tgcatttagt tcgctagcgc tggtgttatg cgggataatt acttcgctga
    30241 tcgcaccgtt ccttttcccg attattctgg cagtaatggg ctaaaatttg cgatgcgtcg
    30301 cgcatttttg atgtaagttt cacgcgttgc ataattaatg agattcagat cacatataaa
    30361 gccataacgg gttcgtaaac tgttatccca ttacatgatt atgaggcaac gccatgcatc
    30421 cacgttttca aaccgctttt gcccaacttg cggataactt gcaatctgca ctggaaccta
    30481 ttctggcaga caagtacttc cccgctttgt tgaccgggga gcaagtctca tcgctgaaga
    30541 gcgcaacggg gctggacgaa gacgcgctgg cattcgcact acttccgctg gcggcggcct
    30601 gtgcgcgtac gccattgtcg aattttaatg ttggcgcaat tgcgcgcggt gtgagcggaa
    30661 cctggtattt cggtgccaat atggaattta ttggtgcgac aatgcagcaa accgttcatg
    30721 ctgaacaaag cgcgatcagc cacgcctggt tgagtggtga aaaagcgctt gcagccatca
    30781 ccgttaacta cacgccttgt ggtcactgcc gtcagtttat gaatgaactg aacagcggtc
    30841 tggatctgcg tattcatctg ccgggccgcg aggcacacgc gctgcgtgac tatctgccgg
    30901 atgcctttgg gccgaaagat ctggagatta aaacgctgct gatggacgag caggatcacg
    30961 gctatgcgct gactggtgat gcgctttctc aggcggcgat tgcagcggca aaccgttcgc
    31021 acatgcctta cagtaagtcg ccaagcggtg tcgcgctgga atgtaaagac ggtcgtattt
    31081 tcagtggcag ctacgctgaa aacgccgcat tcaacccgac tctgccaccg ttgcagggag
    31141 cgttaattct gttgaatctc aagggttatg attacccgga tatccagcgc gcggttctgg
    31201 cagaaaaagc cgatgcgccg ttgattcagt gggatgccac ctccgcaacg ctgaaagctc
    31261 tcggctgtca cagtatcgac cgagtgcttc tcgcttaagc ctggtgccgg atgcggcgtg
    31321 aacgccttgt ccggcttgcc agccctctcc tggtgtcgaa attcccggca aacagtttgc
    31381 cgtttcttgc gcaaaaccag cgggtaaagt agcctgatgg aaattttcct tagatcgagt
    31441 ctcctgcatg ttaaagcgcg tgttcctcag cctgttagtc ctgatcggct tgctgctgtt
    31501 gactgtgctc ggcctcgatc gctggatgag ctggaaaacc gcgccttata tctacgacga
    31561 attgcaggat ctcccctacc gccaggtcgg tgtggtgctc ggaacagcaa aatattatcg
    31621 tactggcgta attaatcagt attatcgcta ccgcattcaa ggagcgatta atgcctataa
    31681 cagcggtaag gtaaattatc tattactgag cggcgataac gcattgcaaa gttataatga
    31741 gccgatgacc atgcgcaaag atttaatcgc tgctggtgtc gacccatcag atattgttct
    31801 cgattacgca ggctttcgta cgctggattc catcgtgcgt acacgcaaag ttttcgatac
    31861 taatgatttc attattatca cccaacgttt ccactgtgag cgggcattat ttattgcgct
    31921 gcatatgggg attcaggctc agtgttatgc cgtaccgtca ccgaaagata tgctgtcagt
    31981 acgtattcgc gaatttgccg cccgtttcgg tgcgctggct gacctttata tttttaaacg
    32041 tgaaccgcgt tttttagggc cgctggtccc tattccggct atgcaccagg tgccggaaga
    32101 tgcgcagggg tatcccgccg tcacacccga acagttactt gaattacaaa agaaacaagg
    32161 aaagtagtta tggatgtaca gcagtttttt gtcgttgccg tttttttcct tattccgata
    32221 ttttgtttcc gcgaagcatg gaaaggctgg cgcgcaggcg cgattgataa acgggttaaa
    32281 aatgcaccgg aaccggtgta tgtctggcga gcaaaaaatc ccggactctt tttcgcttat
    32341 atggtggcat atatcggctt cggaatttta tctatcggca tgattgttta tcttattttc
    32401 tatcgttaat attttctctt ttctgttaat tattgagaat aattattact tcactcaata
    32461 agctgcggat ataacattct gaaccgcagc tatttgtgaa tcctttcaca gtataaattt
    32521 ccccacgcgc atagccttaa tatcagcacg caatatttat actctaaata attcgagttg
    32581 caggaaggcg acaagtgagt gagtccccag gagcttacat aagtaagtga ctggggtgag
    32641 cgaacgcaga cgcagcacat gcaacttgaa gtatgacgag tatatttagc gttcgcctta
    32701 attactcata gcattaagga agatcacatg ccgcaacaaa attatctgga tgaactcact
    32761 ccggctttta cgcctttact ggcgattaaa gaagcctctc gctgtttatt atgtcatgac
    32821 gctccctgta gtcaggcttg cccggcacag accgatccgg ggaaatttat tcgctcaatc
    32881 tactttcgta attttaaagg cgctgccgag acaattcgcg aaaataatgc cctcggtgcc
    32941 gtttgtgcca gagtgtgccc gacggagaaa ttatgtcaaa gcggttgtac ccgtgccggt
    33001 gttgatactc ccattgatat cggtcgctta cagcgttttg ttactgattt tgaacaacaa
    33061 actgggatgg aaatttatca gcccggtact aaaacgctcg gcaaagtcgc gattattggc
    33121 gcaggtcctg ccggattgca ggccagtgta acactgacaa accagggtta tgacgtcacg
    33181 atttatgaga aagaagcgca ccccggtggc tggttgcgta acggtattcc gcaattccgg
    33241 ttaccgcaat cagtgctgga tgcagagatc gcccgtattg aaaaaatggg cgtgaccatt
    33301 aagtgcaaca acgaagtcgg taacacactc acccttgagc agctgaaagc agaaaaccgc
    33361 gcggtactgg tcaccgtggg gttatcgagc ggttccgggt tatcgctgtt tgagcatagc
    33421 gacgttgaga ttgccgtgga cttcctgcaa cgtgcacgac aggcgcaagg cgatatcagc
    33481 attccacaaa gcgcattaat tatcggcggc ggtgatgttg cgatggacgt agccagcacg
    33541 ctgaaagttc tcggctgtca ggcggtaacc tgcgtagcgc gtgaagagtt agatgagttt
    33601 ccggcaagcg aaaaagagtt tgccagcgcc cgggagctgg gcgtttcgat catcgatgga
    33661 ttcacgccag tagccgtgga aggcaataaa gtcacgttta agcatgtacg gttaccgggc
    33721 gaactgacga tggcggcaga taaaattatt ctcgccgtcg gtcagcatgc cagactggat
    33781 gcctttgcgg agttagagcc gcagcgtaac accatcaaaa cacaaaatta tcagacccgc
    33841 gatccgcaag tctttgctgc tggcgatatt gttgagggtg acaaaaccgt ggtctatgcc
    33901 gtgaaaaccg ggaaagaagc cgccgaggcg attcatcact atttagaggg agcttgctca
    33961 tgttaacgaa agatctttcg attacttttt gcggcgtgaa gtttcccaac ccgttctgcc
    34021 tctcttcttc gcctgtaggc aactgctatg agatgtgtgc caaagcctac gacacaggtt
    34081 ggggcggcgt ggtgtttaaa acgatcggct tttttatcgc caacgaagtc tcgccgcgtt
    34141 ttgatcatct ggtgaaagaa gataccggtt ttatcggctt caaaaatatg gagcagattg
    34201 ctgaacatcc gttggaagag aatctggccg ccctgcgtca gctgaaggaa gattacccgg
    34261 acaaagtatt gatcgcttcg atcatggggg aaaatgagca gcaatgggag gagctggcgc
    34321 gcctggtgca agaagctggc gcggatatga tcgagtgtaa cttctcctgt ccgcaaatga
    34381 cttctcatgc gatgggtagc gatgtcgggc aaagcccgga gctggtagaa aaatattgtc
    34441 gggcagtgaa acggggttcc acgctgccaa tgctggcgaa gatgacgccg aatatcggtg
    34501 atatgtgcga agtggcgctg gcggcgaagc gcggcggcgc agatggcatt gcggcgatta
    34561 acaccgttaa atccatcacc aatatcgatc ttaatcagaa aatcggtatg ccgatcgtta
    34621 acggaaaatc gagtatttcc ggatattccg gtaaagcggt aaaaccgatc gccctgcgct
    34681 tcattcagca aatgcgcacc catccagaac tgcgcgattt cccaatcagc ggtatcggcg
    34741 gcattgaaac ctgggaggat gcggctgagt ttttattgct cggcgcagca acgttacagg
    34801 tgaccaccgg catcatgcag tacgggtatc ggatagtgga agatatggca agcgggttgt
    34861 cgcattatct cgccgatcag ggatttgatt cgctgcagga gatggtaggt ctggcgaata
    34921 acaatattgt cccggcggaa gatttagacc gcagttatat tgtctatccc cgtatcaatc
    34981 ttgataaatg tgttggctgt ggacgctgtt atatttcctg ttacgacggc ggtcaccagg
    35041 cgatggaatg gagcgagaaa acccgcacac cgcattgtaa taccgagaaa tgtgtgggtt
    35101 gtctgctttg tggtcacgtc tgcccggtgg gttgtattga gctcggggaa gtgaagttta
    35161 agaaaggcga gaaagaacac ccggtaacgt tgtaaaacca gagacgcatc cggcatttgg
    35221 ttcctgtcgc cggatgcggc ttgaacgcct tatccggcct acaaaaccat acaaatcagt
    35281 atgttgagct cattgtaggc cggataaaac gcgccagcgt tgcatccggc atttggctcc
    35341 tgtcgccgga tgcggcgtga acgccttatc cggcctacaa aaccacacag atcatgatgt
    35401 tatgtgcgtt tatgaccgaa tgcggaccac aatcatttca cttcttacgc gcgtacttca
    35461 gtgaatccag cgctacggcg aagataataa tcgccccttt gatgatgtac tgccagtatg
    35521 ggtttacgcc gatgtacgtc aggccatagt tgatgacggt aaaaataatt accccggtca
    35581 ccacgccaat caccgtcccc acaccgccgc tgaacgatac accgcctacc acgcacgccg
    35641 cgatagcatc cagttcatac ataaagccga ggttgttggt ggcagagccg atacgtccgg
    35701 cttctaacat cccgccaaag gcatagaaca cgccagacaa cgcgtagatc atcagcaggt
    35761 tcaggccgac gttgacacca gatacttttg ccgcttccgg gttaccgcca atggcaaaaa
    35821 tgttcttacc gaagcgggtt ttgttccaca acacccagac gaacgccacc gcaatcaacg
    35881 cgtagaaggt gatgtaagag agacggaaac tccccagcgc gacaaagccc tgagcaaagg
    35941 tagagaagcc actgtcaaaa ccagaaattg gcgacgcccc gacaaagtcg taatagagcg
    36001 agttgattcc atagacgatg atcatcgtgc cgagcgtggt aatgaacggc gtcacgttga
    36061 gataagcgat aatcagaccg ttgatcaaac cgatcaccgc accaatggca cagacaatca
    36121 gaataaccag cgcaatcggc atcgtcgcca tttccgggaa cactttgttg gcgttatcca
    36181 tggactgcaa taatgtcgca gccaccactg ccgccagccc tacctgacga ccagcagaaa
    36241 gatcggtccc ctgggtgaca attaaccctg ccacaccgag cgcgataata atacgcaccg
    36301 atgactgggt gagaatatta cttaagttca acagacttaa aaatgttggg tcctggaaaa
    36361 taataatcgc cagcaaaact aaaagaacga cgtaaatacc gccctctttc aggtaagtaa
    36421 gaaaactttt cttatttaac gcactcatgg gaagccccta atcttaaagg tgcaaagacg
    36481 caagacgcag aatttcgttt tgcgttgttg tttttgtatc gacaattccg gaaacgagac
    36541 cattgctcat gaccagaata cggtctgtta tccctaacaa ctcaggcatt tcagaggaga
    36601 taataataat ccccttgcct ttcttcgcca gttcggcaat taactgataa atttcaaact
    36661 tcgccccgac atcaataccg cgcgtcggtt catcgagcat taatatttct ggttgcgtta
    36721 gtagccagcg accaataatc accttttgct gattaccacc ggagagcgaa ccaatttgcg
    36781 tccgatgacc cggcgttttt acccgcatcg aatcaatcac ccactgggta tcgcttttca
    36841 tccgcgagtt atccagtaaa ccaactttat ttttgtagtt gcgaatattg gaaattaacg
    36901 agttaaaacc aatatccaga taggcataaa ttcccgttga gcggcgctcc tcagttacca
    36961 gtgcaaatcc gtggtttatg gcttcgttgg cattatggtt attgatctgt ttgccgtgca
    37021 acgtaatggt gccagccgat ttctcgcgaa taccaaataa cgtctcaaca atatcggtac
    37081 gtttcgcccc caccagcccg gcaataccga ggatctcccc tttatgcaga tcaaacgaga
    37141 catcgcgaat cgacggctgg cgcagtgacg tcaggttacg cacctcgagg atgacttcgc
    37201 ccggcttgtt ttctttgtca gggaaacgct gattaagaga acgcccaacc atcatggcga
    37261 tgatcttgtc catcgtcagt cctgccagcg gttcggtggc gatccactga ccgtcgcgca
    37321 atacggtaac ttcatcacat aactggaaga tttcttccat tttatgcgag atataaacaa
    37381 taccgcagcc gcgctctttt aatttacgaa taatagtgaa cagatgattg acctcttttt
    37441 cggttaacga agaagtcggt tcatccataa tcacaatttt cgcgttatag gaaaacgctt
    37501 tggcgatttc gatcatctgc atttgcgaaa cggataatgt gccgacgcgc gcacgcggat
    37561 cgatatcaat atccagttca tcaaaaatcg ctttggtttc gcggtacatt ttgtcctgat
    37621 cgacaaacat gcctttggtg ggatatcgcc ccagccacat gttatccatc accgaacgtt
    37681 gtaataccag gtttaactcc tggtgtacca tcgaaatacc attttccagt gcctctttgg
    37741 cggaatggaa atcgatctct ttaccctgga ataaaatggt gccggagtct ttttgataaa
    37801 taccaaacag gcattttaat aatgtcgatt ttcctgcacc gttttccccc attaatgcat
    37861 ggatagaatg tggccggact tttaaattaa cgttatcaag tgccttaaca ccaggaaagg
    37921 acttgttgat accgctcatt tccaacaagt attccccgga agacggagtc gttgagctga
    37981 ccatataatt ttaccttgtt ggccatacaa taagggcgca gtaaacgact gcgcccaatc
    38041 agtcttattt cttgctgaat tcagccaggt tgtctttatc tacgccaaca taaggtacgc
    38101 ggaccacttt gttgtcgatt ttccagttgg tgccatcagc cgcacctttg ccatcggcca
    38161 ggtttttcgc cagatcaaag gtcgctttcg cctggttgtt agcatcgttc agtactgtgc
    38221 ccgccagtgc accggatttc accagcgcca gcgcttctgg caacgcatcg acgccaaaca
    38281 ccggaatgct ggacttgttg tgtgctttca gtgcttctac cgcgcccatt gccatcgcat
    38341 cgttgttggc gataaccact tcgattttgt tggcgttcgg gccagacagc caggcgtcca
    38401 tcttatcttt cgcctgagcg gtatcccaca tagcggtatc taactgtaac tgttcagttt
    38461 taatgccttt gtcgttcagc tctttaatca cgtaagtggt acgtgcttct gcatccggat
    38521 ggcccggttc acctttcagc aatacgaatt gaatctgacc gtctttgttc agatcccaac
    38581 cctgattcgc cgcccagtgt ttagcaatca aatcgccctg aataatgccg gactctttgg
    38641 aatcagtgcc aacgtagtag gctttgtcgt agctatccag cgccttacga gacggttctt
    38701 tgttgaagaa aaccaccggc acgttttgcc cacgcgcttt ctcaatcacc gtacccgcag
    38761 ctgccgggtc aaccaggttg attgccagtg ccttcacccc tttcgccagc aatacgtcga
    38821 tctgatcgtt ctgcttggac tggtcattct gagaatcatt catcagcagc tgaacatctg
    38881 gcgcggcttt cgcatcttgc tcaatagcct tgcgcactac agacataaag ttatcgtcgt
    38941 acttatagat tgttacacca atgcgagtat cagcagcgtg tgcagcggca ccgaataaca
    39001 tgctggccat cacagcagac agggttaaca ccttcttatt catggtatct ccggtttttc
    39061 ttatgcaggg tagtgcttga gataaatgct cggcggggca gtagagttaa tgaagtgtta
    39121 ctgaacgccg aagctcactt tttaaaattc gttcttccat gcccggtaac gctccagaaa
    39181 acggctttaa ttgttgttat gacgctatta cctcggtgaa agtgattatt cactgttaca
    39241 tacgggttac aacgttaaaa cggtgcaatc atagctatca cattgttaag atactgtgaa
    39301 atcactcaca gattgaaagc ggttacatcg cctgatttgt tgagttagtg atcgtcgccg
    39361 cattctggcg tgttgcgaca gaatggcgac gcactaacgt cggcataaaa cagtggctag
    39421 cacgaggatc aatattgcct gctgccccct gcaaggccag ttcggtggct aatttcgcca
    39481 ttgaagcaat gggataacgc acggtcgtta attgcgggtc ggtgtaacgg gcgatgggaa
    39541 tatcatcgaa accgatgatt gagagatgta acggaatcgc aatgccatta tcttttaatg
    39601 ctgtcagtgc gccagcagcc atattgtcgt tataagcaaa tacagcggta agttgtagat
    39661 tgcgccccag cagttcaacc atcgccgcct caccgcccgg catgtccggc gtaccagtgc
    39721 caatccagct ttccggcgga ataatatcct gctctttcaa cgcactcatc cagcctgctt
    39781 tacgcatggc gtcatcttca atgccgtgac tggaagaaag ataaccaata cgttgatgac
    39841 cgttattcag caacatgcgc gtcgccattc gggcaccgct gagattatcc aggcaaacgc
    39901 aacgatgggc gtaccccggc acaacgcggt tgattaacac cataccggga atgttatcca
    39961 taaattgcgc cagttcatcg tcactcaatg cttttgagtg aacaatcaac gcattacaac
    40021 gctggcgaat taacacctca atggcgtgac gctctttttc cgcttcatga tagctattgc
    40081 cgattagcac gtatttctga tgctgctgag cgaccagatc caccgctttt accagcgcgc
    40141 cgaaaaacgc atcagaaacg tccatcacca ccacgccaat ggtgtcgcta acctgagttg
    40201 ccagcgcctg ggcattggcg tttggccgat aatccagctc actcacggct ttcattactg
    40261 cttcacgcgt gtcggcactg accagcgtgc tgttattgag cacccgggaa accgttgcca
    40321 cagagacgcc agcctgacgc gctacatcac gaatggtgat catattcact acctgttatg
    40381 agattgcagt aaatgactgc ttgctggcgg ctattttgtc agcactgaat acaggacttc
    40441 gtgaatcgag tcacagcaat ggaaacggtt acagccgttt tgttaacgaa tgtgatccag
    40501 atcgttatct tgatgtttta gatattgccg ttccggcagc acgcaaagtt aactgacgcc
    40561 agagccattc caccggcccc tggcggaagt aacgcagcca gataacagag aagaggatat
    40621 tcgccagcca taccggaata acaaacgcca gcagctccag gcggtcaaaa tgcataaaca
    40681 aaccgaggtg gtaaaaaagc atggtacaaa tcagcgtttg caatagatag ttggtcagcg
    40741 ccatccgtcc gacgcaggcg atcgcaagca ccagcttaaa gcggctcaat tgcggccaga
    40801 agccataaaa cagcgacgca tagccaatcg cctgaaacgg cgcactcagt tcccgcggca
    40861 tttgaagtaa gaaggcacac cagcgatacg cccagtccag ctgccattgc agggcgatgg
    40921 caggaaggtt aatggtcacc ccaatcgcca ccagcacaaa accagtacga cgatagtgac
    40981 gtaagctgaa ctgccctttc agccagccgc tgcgcatcag cgccgcacca atgagcatca
    41041 tcccagccag ttgccagcca tactgtgcgc ccagtgccag taaactgttg ccaacaccat
    41101 cggcacgatt actgatcgca tcaacgccgc cgtgaagctt ccagtatttt tcatacaaaa
    41161 tagccgatgc atccggcgtc caggcgcggc tggtctggct gtcggaaatc agccccaata
    41221 acagcaaaac gccaagcccc accagataaa gcatgacgcc ggtattaaac aggcttttta
    41281 ccgatggcgc atcgcgcacc agccgccagc agattaagcc caccagcccg taagccagca
    41341 gaatatcgcc gtcccagaac aataaaccgt gaataaagcc caataagact aacagcgtta
    41401 accgcgactg gatccagcgt ctgccacggg gcagcaacat ttgcagtcca gcaccaaaca
    41461 gcagcgcaaa aagcgtgagg aatttcacct ggccgatgag atcaagaaat gcccaggtcc
    41521 atgcatcctg cggcgtaata gcaccgtacc aggcgggatt gagataagcc gcctttggta
    41581 gcccaaaggc gctgatgttt aatagcagga tccccagaat ggcgacgccg cgaacaaaat
    41641 cgagcgtgac gttgcgctcc atggttcctg ccttttaatc agttgtgatg acgcacagcg
    41701 cgcagaaact cgtggcgcgt attctgactg gatttgaaca atccaccaag agaggtcgtt
    41761 gttgtggcac tggttgcatc gcggatgcca cgcgccttca cgcagtaatg caccgcgtcg
    41821 atcgagacag ccacgttatt ggtgcccagc agcgtttgta gcgcaataag aatttgctgc
    41881 gtcagacgtt cctgcacctg cggacgctgg gcaaagaact gcacgatacg gttaattttt
    41941 gacagaccga tcaccgaatc tttcgggata taagccaccg tcgctttgcc atcgatggta
    42001 acaaaatggt gttcacaggt actggtcaga gtgatatcgc gcacggtgac catttcatcg
    42061 accttcattt tgttttcaat gagggtgatt ttcgggaagt tggcgtaatc cagaccggag
    42121 aaaatttcat cgacatacat tttagcgatg cgatgcggcg tttccatcaa actgtcatca
    42181 gccaggtcga gattcagcag ctgcatgatt tcggtcatat gaccagcaat aaggcttttg
    42241 cgcgtttcgt tatccatttc atgcacgggc gggcgcagcg gtgtttccag tcctcgcgca
    42301 actaacgctt catgaaccag ggccgcttct ttactgagtg atggcattta tgatttctcc
    42361 tgcaggtgtg acgcctccgc cctgcgtggg ggcaaagtta ttaagctgat ttacagcctg
    42421 attattgtgc gtgaggcggc gcacataatc cagtattcac agcgataatt attgtaattg
    42481 ccgctgcctt tcatcagcag atgttaaaac atcgttatgc aaatacggaa gtgaaagtta
    42541 ctcacagcac attgaataaa cggtatgatg aagaaattgc aaacaacaca acaaggagcc
    42601 acgcatggaa atgctcgaag agcaccgctg ttttgaaggc tggcagcaac gctggcgaca
    42661 cgactccagt accttaaact gcccgatgac gttcagtatc tttctcccac cccctcgtga
    42721 tcacaccccg ccaccagtgc tgtactggct ttccggatta acctgcaatg acgagaactt
    42781 caccaccaag gcgggtgccc agcgcgtggc ggcagaactg gggattgtac tggtgatgcc
    42841 agacaccagc ccgcgcggcg aaaaggttgc caacgacgat ggctacgatt taggccaggg
    42901 cgcaggcttt tatcttaatg ccacgcaacc gccgtgggcg acgcattacc ggatgtatga
    42961 ttatctgcgc gatgaattac cggcgctggt tcagtcgcaa tttaatgtca gcgaccgctg
    43021 cgccattagc ggtcactcaa tgggtggtca cggtgcgctg attatggcgc tgaaaaatcc
    43081 gggtaaatac accagcgttt cggcctttgc gccaattgtg aatccgtgca gcgtcccgtg
    43141 gggaatcaaa gcgtttagca gctatttagg tgaggacaaa aatgcatggc tggaatggga
    43201 cagttgcgcg ctgatgtatg ccagtaacgc gcaggatgcg atcccgacgc ttatcgatca
    43261 gggcgataat gatcagtttc ttgccgacca gttgcaacct gcggtactgg cagaagccgc
    43321 gcgccagaaa gcgtggccga tgacgctgcg tattcagccg ggatatgatc acagttacta
    43381 cttcatcgcc tcttttatag aggatcacct gcgcttccat gcgcagtatt tactgaagtg
    43441 aaagtccgcc cggttcgccg ggcatcttct catcagaagc gataatccac tgccataaag
    43501 taacgacgtc cgtcttcgtt atagctgtag tcgtcacgac tgagatcctt gtcgccaagg
    43561 ttcagcacgc ctgcgcgcag tttgacgtct ttagtcacct gccaggccgc gccggtattc
    43621 cagatggtat aaccgcccgg tgttttagcc gtcgcgctgt cggcgcgttt ctgcccggta
    43681 tagtgaccag aaacatagaa tgaccagtct tccagcgcca gcggtttcca gtccagcgta
    43741 ccgttagcag tatggaacgg cagatcggat agcggtttgt tttcgccgtt gctgacatca
    43801 cgaccatcgt tgtaggtgta gttgatcgac agtttccatt catcgttgaa cggaattttc
    43861 agttcggttt ccacgccctg aatacgagct ttgttaacgt tgtagtagct aaataccggt
    43921 atgcgccgtc cgttagcgcc cgtctcaaaa ccaacaaagt tttggtagcc cggtgcagcg
    43981 ttgacgtcag acgtacggct gatgctgata cgatctttca catcgttacg gaaaacggta
    44041 acgctggatt caaccccttc cagccagcct tcttcaccca tgtagtaaag ccccagctcc
    44101 caactttcgc tggtttctgg tttcagatcc gggctaccca caatcttaca tgcgccacgg
    44161 caggaattgc tcgtccagtc agggctaagt tgcaacagag aaggtgcttt aaatgccgtc
    44221 gcccagcccc ctttcaccgt tacggtgtcg gtggcgttat aaaccaggta ggcacgcgga
    44281 ctccagtgtt caccgtaggt ttcgtgatcg tccatacgca cgccggtcgt cagcgccagc
    44341 ggctcgaaga tccgccattc atcttccaca aacagcgcgt actggctggc agacgttttg
    44401 gagctggttc ccccggtcag gttcaccgca tcgctaagtt tgtcgtgacg ccattcaccg
    44461 ccaaccgtga gaaactgatt aatcgccgtc agcggcaacg tgtatttgcc gtcgaccgta
    44521 ttgctttcgg aagttatcgg gctgctgttg ccagggtttt tgttctcgac tttctcaccg
    44581 tagtatttca gttcgctggt gccgtaatcc caacgcccat tatggctgac ggagtagttc
    44641 tggcgttcca ggcggttttt gtccagcgag tcggaatcac gatcctgacg gtcgaaaccg
    44701 tatccggcag taaaatcgtg attttgattc ggtgtccagg caaattcgac attgccgtcg
    44761 cggctggaga atccttcaat acgcggcgtt tctccggtat cggtggtcgt tgagttttgc
    44821 gggtcatcct tttcacgttt tgccaggctg ccgtaagctt tcattcccag cacaccatca
    44881 attaatggtc cactggtaaa gaactgaccg ttataggtat caccgcgatc gcgatgttcc
    44941 tgaatggtgg tatcgacggt aacggtaccc gaccatttct gaccgatttt tttggtgatg
    45001 atattcacta caccgccgag cgcatcggag ccgtatagcg acgacatcgg gccacgaacc
    45061 acttcaatac gttcgatgga atcgaccggg atccagttca gatcgaaatc attgtggcgg
    45121 aagacggcat tgcgggagtt aacgcgttta ccgtcgacga gaatcagggt gtagctgctg
    45181 tccagaccac gaatactaac gcccttacgg ttatcccctt cgttcgtcag ttgtacgcca
    45241 ggcacttctt tgaggacatc cttcagattc tgtaccggtt ttcgctgcag gtcttcctgg
    45301 gtaatgacgc tgatactggc gggtgcatct ttgaggtttt gttcaacgga agatgcagtg
    45361 acaaccatcg tttcgccatc atcatcgacc gctaacacag gccatgcaca agaaatagcg
    45421 gacaaacaca gcccgacccg tacgaaaggg ttcaacctaa acattccata tctccatgag
    45481 gtaactacga aaataaaatg ggttatcgct cacatcttct tcacgtcccc ttgcgtacgg
    45541 cagcatcgcg gtggacttat ttgtatgcag gtgatcatcc cagaaagccg gaacagcctc
    45601 tgatttgtca gccttttttg attgcggcgt aacgataatg caaacgataa caattatcaa
    45661 tccaaatgtt aaattttata tccgcatgat ttgtcaggga aatggaattg aaaaagccct
    45721 ctcggttgag agggcttagc aaggaaggga ggaaacttat ttcttatcgt tctgcgggaa
    45781 cttcatttcg ctgtagcgta cgaagtgagt tcctttaatc agcttgtagc cgaaccaaat
    45841 aatcaggaac agcgggatac caatatacgt tgccgctacg ccgccccagt caatggtatc
    45901 tttcaggaac gcttcgtagt tctggcccaa agtgataatc agacacagaa tgaatgcgaa
    45961 gatcggcccc agtgggaaga aacctgaacg gtacggcaga tcgttaatgt cgtgtccctg
    46021 caatacgtaa ccgcgacgga agcgatagtg gctaatggca atccccagcc aggcgataaa
    46081 acccgtcatc ccggaggtgt tcagcagcca caggtatacc gtctggttgc caaacatgga
    46141 ggtcaggaag cacagaccgg caatcaccgt cgtcgcatac agcgcattac gcggcacgcc
    46201 accacgcgac agtttagcga aaatgcgcgg cgctttaccg tcacacgcca gggtgtacag
    46261 catacgagta gacgcataca taccggagtt acccgctgac agcaccgccg tcagaataac
    46321 tgcgttcatc accgccgccg cagagagcag acccgcgtgc tggaacacca gggtgaacgg
    46381 actaacgctg atgtctttaa catcgttacg cagcaggctc ggatcggtgt acggaataat
    46441 caggctgata atcaggatcg cgaacacata gaacaacagg attcgccaga acacctgacg
    46501 taccgcgcgt ggaatgtttt tcgccggatc ttcggactcg cctgcagcaa taccgatcag
    46561 ctcggttccc tggaaagaga agccgacaat catagctacg ccgatcatcg ccgcaaaacc
    46621 accagcaaac ggcgcttcgc cgattgtcca gttgctccag cccgcaggct gcgcgccttt
    46681 gaagataccg ataatcatca gcacgccaac gatgataaag acaataactg tcgtgacttt
    46741 gatcagtgag aaccagtatt ccgcttcacc aaagccacga actgagatgt agttcagcag
    46801 gaagataacg ccgaggaaca acgcactcca gatccagccc ggtgtatccg ggaaccacca
    46861 gctcatgacc agctgagctg caaccaggtc aacggcgata gtcaccgccc agttgtacca
    46921 gtagttccag cccagcgcga agccaaagcc ttcttcaaca tagttctgac cgtaagtggc
    46981 aaacgaaccg gaaaccggca tatatgcagc cagttcaccg agactggtca tcaggaagta
    47041 aaccatcagg ccaatcagca tatacgagag caatgccccg cccggacctg cctgagaaat
    47101 cgttgcgcca gaggcaacaa aaagacctgt accgatggaa ccgccaatgg caatcatcgt
    47161 caggtgacgc gcctttaatt cacggcgtaa gcccggcgct tctgtggttt tagtttcgga
    47221 aaccatacaa aaatgctatc catcttaaaa atgaggcgcg attgtagcag acgattggca
    47281 atccttccgg cagaaataca cggttataag acaccttcat gatcgcccag ggattataag
    47341 taaagcagcc aatctctttt ctggcgaatg catgctgagg gcgctgcgtc acgaaatcga
    47401 cacagcacca gcatgttctt gtacagcaac ttaacgcggt acattcgcgg gatcgcaata
    47461 gtccagaaag cgccgtagcg cgttggaaag gtgtttttgc cgatgatgta tacgccacaa
    47521 cgtacgcatc aggcgcggca gagggaccgc aacttcactt aatgtgcctg cctgcaattg
    47581 atcttcaatc acccgtcgcg acaggcaact aattcccaac ccatgacgca ccgcatgttt
    47641 gattgcctcg gagttaccta attccatcgc catctcaaac ttcggtaaat gtgacagcaa
    47701 tagataatcg acaatctccc gcgtgccgga accgcgttca cgcaggatcc acggcgcagc
    47761 ggccagctgc tctaaggtga ccggaccacg ggccaacggc gaagtcggcg cggcgaaaac
    47821 caccagctcg tcttccagcc acggttcaga aataatttca gtgctgtggc acggtccttc
    47881 aataaaacca atatcaacgc ggaaatccag caccgcttgc atcacgtcct ggctgttccc
    47941 aacgctaagt tcaatcggca actgcggata atcatggcga taacgggcga taactgcagg
    48001 cagaatgtag ttaccgatgg tactactggc atagatacga atcgcgccgt tgtcttcgcg
    48061 aaacagttgt tcgatttcaa ccgcctgttc aagcaatgcc aacgcacgcg gatagagcag
    48121 ccgcccgtgt tcattaacaa ccagtctttt ccccacgcga tcaaacagtt gcacgccaag
    48181 ctgcccttcc aggtcggtca aggctgcgct cactgctgat tgcgacaacg ccagcatcac
    48241 cgacgcctgg gtggttgatc cacttttcaa tacttctgca aaaacttcca actgccggag
    48301 ggtgatgtgc atagtcgctt accacttata aagattaatt ataaatatat aatcaatttt
    48361 atttttaaac cagttagtcg taaccttatg cccggttaaa ggagagggtt atgacgaata
    48421 tcaccttaca gaaacaacat cgtacactgt ggcattttat tccggggtta gccctgagtg
    48481 cagttatcac cggggtcgcc ctgtggggtg gttccattcc cgcggttgcg ggtgccgggt
    48541 ttagtgccct caccctcgca atcttgttgg ggatggtttt aggcaacacc atctatccgc
    48601 acatctggaa aagctgtgac ggtggtgtgc tgtttgccaa gcaatattta ttacgtctgg
    48661 gtatcattct ttatggcttc cgtctgacgt tctcgcaaat tgccgatgtc ggtatcagtg
    48721 ggatcatcat tgacgtgctg acgctgtcca gtaccttcct gctggcttgc ttcctggggc
    48781 agaaagtgtt tggtctggat aagcacacca gctggttgat cggtgccggt agcagtatct
    48841 gtggtgctgc cgcggtactg gcgactgagc cggtagtgaa agcggaagcc agtaaagtaa
    48901 ccgtggctgt tgcaaccgtt gttatcttcg ggaccgtcgc gattttcctc tacccggcga
    48961 tatatccgct gatgtctcaa tggtttagtc cggaaacttt cggtatctac attggttcta
    49021 ctgtgcacga agtggcgcag gtggtggcgg caggtcatgc catcagcccg gatgcggaaa
    49081 acgcagcagt tatttccaaa atgctgcgcg tgatgatgct ggctccgttc ctcatcctgc
    49141 tggcggcgcg tgttaaacag ctgtctgggg cgaacagcgg cgagaaaagc aaaatcacta
    49201 ttccgtggtt tgccatattg ttcatcgtcg ttgccatctt taactcgttc cacctgttac
    49261 cgcagagcgt ggtgaacatg ctggtaacgc tggatacctt cctgctggca atggcgatgg
    49321 cggcgctggg tctgaccact cacgtcagcg cgctgaaaaa agccggggcg aaacctctgc
    49381 tgatggcact ggtactgttt gcctggctga ttgttggtgg tggtgcgatt aactatgtga
    49441 ttcaaagcgt catcgcataa accactacat cttgctcctg ttaacccgct atcattaccg
    49501 ttttcctcca gcgggtttaa caggagtcct cgcatgaaat acattggagc gcacgttagt
    49561 gctgctggcg gtctggcaaa tgccgcaatt cgcgccgccg aaatcgacgc aaccgcgttt
    49621 gccttgttca ccaaaaacca acgtcagtgg cgtgccgcac cgctcacgac gcaaaccatc
    49681 gatgaattca aagcggcctg tgaaaaatat cactacacat cggcgcaaat tcttccccac
    49741 gacagctatc tgattaacct cggacatccg gtcactgaag ctctggaaaa atcgcgcgat
    49801 gcctttatag atgaaatgca gcgttgcgaa caactggggc tttctttgct caacttccac
    49861 cctggcagcc atctgatgca gatttcagaa gaggattgcc ttgcgcgtat tgccgaatcc
    49921 atcaacattg cgctggataa aactcaaggt gtaacagcgg tgattgaaaa caccgccggt
    49981 cagggcagta acttagggtt taaattcgaa catctcgcgg cgattatcga cggcgtggaa
    50041 gataaatccc gtgtcggcgt ctgcattgat acctgccatg ctttcgctgc cgggtatgat
    50101 ttgcgtactc cagccgaatg cgagaaaacg ttcgcggatt ttgcccgtat tgttggcttt
    50161 aagtatctgc gcgggatgca ccttaacgat gcgaaaagca cctttggcag ccgcgttgac
    50221 cgccatcata gcctcggtga aggcaatatc ggtcatgatg cgttccgctg gatcatgcag
    50281 gacgaccgtt tcgacggcat tccgctgatc cttgaaacca tcaacccgga tatctgggca
    50341 gaagagatcg cctggctgaa agcgcaacaa actgaaaaag cggtggcctg atcgatgaac
    50401 gacagggaaa agcagattct taaaatttta cggcgtaacc cgctgattca acagaatgaa
    50461 atcgccgata ttctgcaaat cagccgctca cgtgttgccg ctcatattat ggatttgatg
    50521 cgcaagggtt tgattaaagg caaaggatat atccttaccg agcaggatta ctgtgtggtt
    50581 gtgggtgcaa tcaatatgga tattcgcgga atggcggata tccgttaccc gcaagcagcc
    50641 tctcatccag gttctgttca ctgttccgca ggcggcgtcg gacgtaatat cgcgcataac
    50701 ctggctctgc ttggtcggga tgtgcattta atatcagcta ttggcaacga tttttacggc
    50761 gagacattac tggaagaaac gcgccgcgcg ggcgtgaacg tttcaaactg cattcgtctg
    50821 catggacaca gcactgcaac ttatctcgct atcgctaata agcaggaaga aactatcctg
    50881 gcaatcaatg acacacatat tctgcaacag ctaacgcctc aactgctcaa tacttcacgg
    50941 gacttgattc gccatgctgg tgtggtgctg gctgattgta atctcacacc tgaagccctg
    51001 gagtgggtct ttaccattgc tgatgaaatt ccgatgtttg tcgataccgt ttccgaattt
    51061 aaagcgaaca aagtaaaaaa ctggtacagc cggatccaca ctctgaaacc aacacaaaat
    51121 gaactggaaa ttttgtgggg gcaaccgatt aaagacgaca acgatcgcat cagagctgta
    51181 aattcgctgc atcaacaagg tgttaaacgc atttttgttt atctgaaaga tgaatctgta
    51241 ttttgcagcg ataaggatgg agaacaattt ttgctgaccg cgccagcgca tacgacagta
    51301 gacagttttg gtgctgacga tggttttatg gcgggcttgg tatatagctt tcttgaagga
    51361 tacagtttcc gcgacagcgc ccgttttgcg gtagcctgcg cggcaatttc gcgcgccagc
    51421 ggcagcttaa acaaccctac cctgtctgcc gataacgcgc tttcattagt gccaatggtg
    51481 taacaatgtt gccggattcg acgcttgacc gcatcttatc cggctcataa attgcgcact
    51541 acgctaaacc aataaagaac ccggcaatgg tggcactcat cagattagaa agtgtcgccg
    51601 ccgccagcgc gcgtaaacca agctgggcga tttccggcgc acggtgtggc gcaaccgcag
    51661 aaaacgcccc caccaccacc ccgatagaac caaagttagc gaaaccgcac aacgcgaagg
    51721 aaataatcgc cacggtttta gcatccagag tgccagccgt ttgcagatag ggtgagaaat
    51781 tgagataagc gacaaattca tttattgcca gtttctgtcc aatcaaactc ccggcaagat
    51841 ttgcatcact ccagtcaaca cccattaccc acgcaagtgg ggccagcagg taacctaaaa
    51901 tggactccag cgaggcatgt tcaaaaccaa accagccgcc aacgccgccg ataataccgt
    51961 taattaacgc aatgatggcg acaaatgcca taacaactgt cgctacaccg gcggcgattt
    52021 tcagcccggt cattgcccct gtcgcagcgg cttcaataat gctttttggc ggtgtttcgg
    52081 tgaaagagat gttattaaag gaaacctgag aagattccgt tgccggactt aacaggcggg
    52141 caaacaagat cccgccaggg atcgccatta acgatgccgc cagcagatat tccacaggca
    52201 cgcccagtgc ggcgtaacca atcattgtcg aaccagcaat cgaggccatg ccactacaaa
    52261 tcgctgtaaa taattcattg cgattcagcc gatcgataaa gggtttgacg attgccggaa
    52321 ttttgttttg cccgaggaaa atggtagtga ccgcgacgaa tgactcgatc ttgctgatat
    52381 ttaacgcttt ctggaagata ccgccgagaa tgcgaattaa aatccccatc acaccgatgt
    52441 agtagagaat actcaccagt gctgtaacga agataattgc cggtaatact ctgaaaccaa
    52501 agataaatcc tgcgccatca aacagcgtgt ccattttcgg cccgaccagc gaaccgaaga
    52561 taaatgcgct acccgcgtcg ctgtacgcca tcactttatg cacgccaaaa gcgacttttt
    52621 cagcgaccca acgccctggc ggtaaccaaa gcataatgcc gccaatcaca acctgtaaca
    52681 ctaacgccgc gccaacggta cgcaggctga tcttcttctt gtttactgac agcaaaaatg
    52741 caattgccag caataccacc attcccagaa cacttctcat gacatccata gtgattatct
    52801 cttcatgtca tattgggcga cggctttctg cccatcagaa atagtcgggc ctgcatcttg
    52861 cgctatcggc ccggcttgtt atcaacgctt aatgggtttt gatgtagccg cgcacgcact
    52921 cttcgactaa gccccagaac cagtcggtat caatagtgat gccgactttg gtattggcgg
    52981 gcttgccaag aacgcccagc tcgtcgcaga cggtgcgccc atagcaaggg ccactgttga
    53041 cgtccacttc gacgtacatc tcctgggttt taatgccatc agggttaatc agataaccga
    53101 tgcaggtggc gtcgtgcacc gggccgccag ccaggccgta gttttcgaac tgcgttttga
    53161 gagtgaagtt catgatgtcg ctgaacagct ctccggcagg gccgcctgcc ctttccatcc
    53221 gagcaatcac gtccggggtg caaacggtct ggttggtgag atcgaggccc atcatcacta
    53281 atggaacgcc agaggtgaac accacgcggg ctgcttccgg gtcagcaaag atgttgaact
    53341 cggcagatgg cgtgaagttg cctgtaccgt aagcgccgcc cattagcaca atttcacgga
    53401 ttttgggcag gatcgcgggt tgcatacgca ttgccaccgc gatatttgaa agcggaccaa
    53461 ccggcaccag agtgatatcg ccatcgcttg ccatcagggt atcaatgatg tatttcaccg
    53521 cgtgggtact ttctgcctgg cgggtcagcg gctcgaatac cgggccatcc agtccggttt
    53581 caccgtgaat attatcggca acgatttgtt gacgcataat gggttgcggc atccccgcat
    53641 aaaccggaac attaatctcc agtttctggc aaacattcag gccattaatt aatgttttat
    53701 caagcgtctg attacccgct acaatagtga tgcctaataa atctattgcc ggatgtttcg
    53761 ccgccatcat tatagcaata gcatcatcat gacccggatc acaatccaga ataatttttc
    53821 tcttttccat tgtttatttc ctctgtttcc agttgcgtta ttttttctac agcaaagaaa
    53881 attcgaccgg gccgatgatt gaatcttacc aaccgcgtac gtatgctaaa tatgagaaat
    53941 ctcatagcgg ataaacatcg tgaaagaaat ccacaataat gatcttaagc agcaattgat
    54001 gagtgaatcc gcgtttaagg attgcttttt aacggatgtt tcagccgata cgcggctgtt
    54061 tcatttttta gcgcgtgact acattgtgca ggaagggcaa cagccgtcct ggctgtttta
    54121 cctgacgcga ggccgcgcca ggctttacgc cacgctagct aatggtcgcg tgtcgctgat
    54181 cgatttcttt gccgccccct gttttattgg cgagattgag ttaatcgaca aagaccatga
    54241 accgcgtgcg gtgcaggcta ttgaagagtg ttggtgcctt gcgctcccta tgaaacatta
    54301 ccgcccgcgg ttattaaacg acacgctatt tttacgaaaa ctctgcgtca ccttaagtca
    54361 taaaaattat cgtaatattg tttctttaac tcagaatcaa tcatttccgt tagtgaatcg
    54421 cctggcagca tttatattac tctcgcagga aggtgatctt tatcacgaaa agcatacgca
    54481 agcggcagag tatttaggcg tttcttatcg acatctttta tatgttctcg cgcaattcat
    54541 tcacgacggt ttattaatta aaagcaagaa agggtatcta attaaaaaca gaaagcagtt
    54601 gtcaggactg gcgctggaga tggacccgga gaataaattc tccgggatga tgcagtaaaa
    54661 attatttgca atagcgcgat tgccggatgc aacacttaat aagcgcatac atccggcaat
    54721 gaaactcaag ctaaaccaat aaagaacccg gcaatggtcg cactcatcag gttggaaagc
    54781 gttgctgctg ccagcgcccg taaaccaagc tgggcgattt ccggcgcgcg ttttggcgaa
    54841 atagccgaaa atgcgccaac gacaacaccg atagaaccaa agttagcaaa accacaaagc
    54901 gcaaaggaga taatcgcaat ggttttcact tccagcgtgc cgcccgtttg caggtatggg
    54961 gagaaactca ggtaagcgac gaattcgtta atcgccagtt tctgcccaat caggctaccc
    55021 gcaagattgg catcactcca gtccacaccc atgatccacg ccagcggtgc cagcacatag
    55081 ccaaaaatac tttccagaga ggcattggcg aaaccaaacc agccaccaat tccgccgata
    55141 atgccgttga tcagcgcaat aattgcgaca aacgccatta ccaccgtcgc tacaccagcg
    55201 gcgattttca gcccggtcat cgcaccgctc gccgccgctt cgataaagct ttttggcggc
    55261 gtttcgctga acgacagatt ttcaaatgtg acctgtgaag gctcggtggc cgggctaaga
    55321 atacgtgcaa acaaaatacc acctgggatc gccatcagcg atgccgctaa caggtagtca
    55381 attggtacgc ccattccggc ataaccaatc atcatcgaac cagcaatgga cgccatcccg
    55441 ctacaaattg cggtaaacaa ctcgttgcga ttcatgcgat cgataaacgg tttaacgatc
    55501 gccgggatct cattttgccc gaggaaaata gttgtgactg cgacaaaaga ttcgattttg
    55561 ctgatgttaa gagctttctg gaaaatgctg ccaaggatgc gaatcagcag ccccatcacg
    55621 ccaatgtagt acagcagact gatgagcgca gtaacgaaaa taatcgccgg aagtacgcga
    55681 aaggcgaaga taaaacccgc accgtcaaac aggacatcca ttttcggccc aaccagcgaa
    55741 ccaaaaatga aggcgctacc ggcatcactg taagacatca ctttatgaac gcctaatgcc
    55801 gcctgttcta ctgcccactt tcccggtggg aagtagagca tgatgccacc aatagcgatt
    55861 tgcagcagca gtgcggctcc aaccgtgcgc aaactgatgc tctttttatt cactgacaac
    55921 agaaatgcta ttgccagtaa caccaccatc cccacaacac ttctcattat atccataatg
    55981 attttccctt catgtcagta aacccggcgt cagcgccagg ttttggtatg cttgatgagt
    56041 acgggcgacg gctttctgcc cgtcagaaat taacccgcga gacgctggta ttctttggca
    56101 atttcactcg ccagaatggc gttgttgaac accagctgga tattggattt caggctgtca
    56161 ccgccggtca gctcagcaac gcgcgccagc aggaacggtg tactttcttt accaataacg
    56221 ccctgctctt cggcttcggc gacggcttga tctatggctg cattgataga ttcctccggc
    56281 atcgcaaact gttccgggat cggattcgcc actaccaggc cgccgttcaa accggtttgc
    56341 catttcactg ccattgcacg agcgatttcc gtggcactat caaggcgaat actgacttca
    56401 aatgggctgg tacggcagaa aaacgcaggc agcgcttttg tctgatagcc aattaacggc
    56461 acaccgaagg tttctaaata ttctgttgtt aatcctaaat cgagaataga tttcgccccg
    56521 gcacaaacaa cggtgacatt agtatttgcc agttcttgca aatcggcaga aatatcgaag
    56581 gtatgttccg ccccgcgatg cacaccacca attcccccgg tggcaaatac tttaattccg
    56641 gcaagcgccg caataatcat cgttgaagcc acagtggttg cgccattttt tccggcggca
    56701 acaacaaaag gtaaatcgcg acgactaact ttggtcacgt tatgcccttc acgacccagt
    56761 aattcaattt cttctttgct taaacccact ttcatcacgc cgccaataat ggcgatagtg
    56821 gcaggtactg cgccctgttt acgaatagtt tcttctactt caattgcggt ctgggcattt
    56881 tgtgggaacg gcatcccgtg agaaataatg gtcgattcca gcgccacaac cggttttttg
    56941 ttttttaaag cgtcctgcac ttccggggaa atttgtaata attcagggga aatttttaat
    57001 tcagacattc tgcgttctcc actaacgata taacgttggc aatcgataaa tcggggttat
    57061 tggtgtattc acaggagagc gccattgacg aacatccctg tgcgaaacga acagattcgg
    57121 caaacggcat tccgtctacc caacacgaag caagtcccgc catcatggca tcgcccgctc
    57181 cggtaacatt aataacattg gttttgatcg gggcagacca gccactttca ccgctgatat
    57241 cactgtaata aacgccatcg ccgcccatgc tcaataccag tcggttcagg ccatgttgat
    57301 ggaaccaggc agcaactttt gccacatctt cacgccctga cagcgcaatc ccactcagtg
    57361 tttccgcttc aaggcggttt ggcttcagag tgtggatctg atttagacgg tcgcgcactt
    57421 tgacacattt ccacgctgaa accggatcga caaatacggg tacgttggcg gcattatcca
    57481 gaatccatgc cagtgcctct tcactgatat tacagtctgc gacaatgacc tttgccctct
    57541 gaataaattc acggtgctgt gcgagatatt cagctgtaat agcgttgcta atattcatgt
    57601 catttatagc aaccagcatt tcaccggtat tatcgagtaa tgataaataa ctagacgtat
    57661 tttctcccgg cacaatcagg catttatcga cataaacgcc agattgattg gtttgcgtta
    57721 gcagcgattg accataaaaa tcactgccta ctgcgctcag tagccaggct ttgttaccca
    57781 gcaacgccag gttttgtgca atattgcgcc ctactccacc aggcgtaaat tttattttac
    57841 ctggatttga atccgcataa tttaatgatt catgtgaata tccggcgaca tcaatattcg
    57901 ccgaacctat aattacgaca taatcctttt cgcgcatagc acgtcctctg gtaaaaaatt
    57961 accaaccctg tcaatccaca agaatataga tactgcaaag acggtttaaa
//
LOCUS       DAEVZA010000013        55003 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEVZA010000013 DAEVZA010000000
VERSION     DAEVZA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.013"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            387..1439
                     /gene="aroG"
                     /locus_tag="LAF92_000854"
     CDS             387..1439
                     /gene="aroG"
                     /locus_tag="LAF92_000854"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBI9789907.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(1597..2349)
                     /gene="gpmA"
                     /locus_tag="LAF92_000855"
     CDS             complement(1597..2349)
                     /gene="gpmA"
                     /locus_tag="LAF92_000855"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBI9789908.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(2554..3594)
                     /gene="galM"
                     /locus_tag="LAF92_000856"
     CDS             complement(2554..3594)
                     /gene="galM"
                     /locus_tag="LAF92_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBI9789909.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(3588..4736)
                     /gene="galK"
                     /locus_tag="LAF92_000857"
     CDS             complement(3588..4736)
                     /gene="galK"
                     /locus_tag="LAF92_000857"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBI9789910.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(4740..5786)
                     /gene="galT"
                     /locus_tag="LAF92_000858"
     CDS             complement(4740..5786)
                     /gene="galT"
                     /locus_tag="LAF92_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBI9789911.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(5796..6812)
                     /gene="galE"
                     /locus_tag="LAF92_000859"
     CDS             complement(5796..6812)
                     /gene="galE"
                     /locus_tag="LAF92_000859"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBI9789912.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(7073..8545)
                     /gene="modF"
                     /locus_tag="LAF92_000860"
     CDS             complement(7073..8545)
                     /gene="modF"
                     /locus_tag="LAF92_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBI9789913.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(8613..9401)
                     /gene="modE"
                     /locus_tag="LAF92_000861"
     CDS             complement(8613..9401)
                     /gene="modE"
                     /locus_tag="LAF92_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBI9789914.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            9530..9679
                     /gene="acrZ"
                     /locus_tag="LAF92_000862"
     CDS             9530..9679
                     /gene="acrZ"
                     /locus_tag="LAF92_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBI9789915.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            9846..10619
                     /gene="modA"
                     /locus_tag="LAF92_000863"
     CDS             9846..10619
                     /gene="modA"
                     /locus_tag="LAF92_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI9789916.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            10619..11308
                     /gene="modB"
                     /locus_tag="LAF92_000864"
     CDS             10619..11308
                     /gene="modB"
                     /locus_tag="LAF92_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBI9789917.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            11311..12369
                     /gene="modC"
                     /locus_tag="LAF92_000865"
     CDS             11311..12369
                     /gene="modC"
                     /locus_tag="LAF92_000865"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBI9789918.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(12370..13188)
                     /gene="ybhA"
                     /locus_tag="LAF92_000866"
     CDS             complement(12370..13188)
                     /gene="ybhA"
                     /locus_tag="LAF92_000866"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBI9789919.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            13343..14338
                     /gene="pgl"
                     /locus_tag="LAF92_000867"
     CDS             13343..14338
                     /gene="pgl"
                     /locus_tag="LAF92_000867"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBI9789920.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(14379..15332)
                     /locus_tag="LAF92_000868"
     CDS             complement(14379..15332)
                     /locus_tag="LAF92_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9789921.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            15516..16568
                     /locus_tag="LAF92_000869"
     CDS             15516..16568
                     /locus_tag="LAF92_000869"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBI9789922.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGKVYLP"
     gene            16644..18077
                     /locus_tag="LAF92_000870"
     CDS             16644..18077
                     /locus_tag="LAF92_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBI9789923.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            18260..20521
                     /locus_tag="LAF92_000871"
     CDS             18260..20521
                     /locus_tag="LAF92_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBI9789924.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKNEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIISAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(20755..22038)
                     /locus_tag="LAF92_000872"
     CDS             complement(20755..22038)
                     /locus_tag="LAF92_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBI9789925.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(22190..22666)
                     /locus_tag="LAF92_000873"
     CDS             complement(22190..22666)
                     /locus_tag="LAF92_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBI9789926.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(22725..24014)
                     /gene="bioA"
                     /locus_tag="LAF92_000874"
     CDS             complement(22725..24014)
                     /gene="bioA"
                     /locus_tag="LAF92_000874"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBI9789927.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24101..25141
                     /gene="bioB"
                     /locus_tag="LAF92_000875"
     CDS             24101..25141
                     /gene="bioB"
                     /locus_tag="LAF92_000875"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBI9789928.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNSQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            25138..26292
                     /gene="bioF"
                     /locus_tag="LAF92_000876"
     CDS             25138..26292
                     /gene="bioF"
                     /locus_tag="LAF92_000876"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBI9789929.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            26279..27034
                     /gene="bioC"
                     /locus_tag="LAF92_000877"
     CDS             26279..27034
                     /gene="bioC"
                     /locus_tag="LAF92_000877"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBI9789930.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            27027..27704
                     /gene="bioD"
                     /locus_tag="LAF92_000878"
     CDS             27027..27704
                     /gene="bioD"
                     /locus_tag="LAF92_000878"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBI9789931.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            28283..30304
                     /gene="uvrB"
                     /locus_tag="LAF92_000879"
     CDS             28283..30304
                     /gene="uvrB"
                     /locus_tag="LAF92_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBI9789932.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(30496..31404)
                     /gene="yvcK"
                     /locus_tag="LAF92_000880"
     CDS             complement(30496..31404)
                     /gene="yvcK"
                     /locus_tag="LAF92_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBI9789933.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      31669..31813
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            31801..32790
                     /gene="moaA"
                     /locus_tag="LAF92_000881"
     CDS             31801..32790
                     /gene="moaA"
                     /locus_tag="LAF92_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBI9789934.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            32812..33324
                     /gene="moaB"
                     /locus_tag="LAF92_000882"
     CDS             32812..33324
                     /gene="moaB"
                     /locus_tag="LAF92_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBI9789935.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            33327..33812
                     /gene="moaC"
                     /locus_tag="LAF92_000883"
     CDS             33327..33812
                     /gene="moaC"
                     /locus_tag="LAF92_000883"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBI9789936.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            33805..34050
                     /gene="moaD"
                     /locus_tag="LAF92_000884"
     CDS             33805..34050
                     /gene="moaD"
                     /locus_tag="LAF92_000884"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBI9789937.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMSAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            34052..34504
                     /gene="moaE"
                     /locus_tag="LAF92_000885"
     CDS             34052..34504
                     /gene="moaE"
                     /locus_tag="LAF92_000885"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBI9789938.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            34641..35345
                     /locus_tag="LAF92_000886"
     CDS             34641..35345
                     /locus_tag="LAF92_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBI9789939.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            35547..36194
                     /locus_tag="LAF92_000887"
     CDS             35547..36194
                     /locus_tag="LAF92_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBI9789940.1"
                     /translation="MLLLIWWLTAAGVWATAGCAWIFQHLHWVTSQKSPGAFVFALAA
                     LIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLYTFSSIATVMGITGGMFAAM
                     ALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSFSVWLASILTVFIWGITAAC
                     EATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVLFRITVTVLEFINGLVW"
     gene            36264..36977
                     /locus_tag="LAF92_000888"
     CDS             36264..36977
                     /locus_tag="LAF92_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBI9789941.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(37013..37969)
                     /locus_tag="LAF92_000889"
     CDS             complement(37013..37969)
                     /locus_tag="LAF92_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBI9789942.1"
                     /translation="MSKSHPRWRLTKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(37969..39210)
                     /gene="clsB"
                     /locus_tag="LAF92_000890"
     CDS             complement(37969..39210)
                     /gene="clsB"
                     /locus_tag="LAF92_000890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBI9789943.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(39207..39968)
                     /locus_tag="LAF92_000891"
     CDS             complement(39207..39968)
                     /locus_tag="LAF92_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBI9789944.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            40101..40511
                     /locus_tag="LAF92_000892"
     CDS             40101..40511
                     /locus_tag="LAF92_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBI9789945.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(40473..41579)
                     /locus_tag="LAF92_000893"
     CDS             complement(40473..41579)
                     /locus_tag="LAF92_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9789946.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(41590..42723)
                     /locus_tag="LAF92_000894"
     CDS             complement(41590..42723)
                     /locus_tag="LAF92_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9789947.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(42716..44452)
                     /locus_tag="LAF92_000895"
     CDS             complement(42716..44452)
                     /locus_tag="LAF92_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI9789948.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(44445..45440)
                     /gene="hlyD"
                     /locus_tag="LAF92_000896"
     CDS             complement(44445..45440)
                     /gene="hlyD"
                     /locus_tag="LAF92_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBI9789949.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(45443..46114)
                     /gene="cecR"
                     /locus_tag="LAF92_000897"
     CDS             complement(45443..46114)
                     /gene="cecR"
                     /locus_tag="LAF92_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBI9789950.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            46343..47707
                     /gene="rhlE"
                     /locus_tag="LAF92_000898"
     CDS             46343..47707
                     /gene="rhlE"
                     /locus_tag="LAF92_000898"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBI9789951.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(47939..48421)
                     /gene="ybiA"
                     /locus_tag="LAF92_000899"
     CDS             complement(47939..48421)
                     /gene="ybiA"
                     /locus_tag="LAF92_000899"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBI9789952.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            48541..50691
                     /gene="dinG"
                     /locus_tag="LAF92_000900"
     CDS             48541..50691
                     /gene="dinG"
                     /locus_tag="LAF92_000900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBI9789953.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            50719..51681
                     /gene="ybiB"
                     /locus_tag="LAF92_000901"
     CDS             50719..51681
                     /gene="ybiB"
                     /locus_tag="LAF92_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBI9789954.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            51822..52907
                     /gene="hcxB"
                     /locus_tag="LAF92_000902"
     CDS             51822..52907
                     /gene="hcxB"
                     /locus_tag="LAF92_000902"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBI9789955.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(53136..53396)
                     /locus_tag="LAF92_000903"
     CDS             complement(53136..53396)
                     /locus_tag="LAF92_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI9789956.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(53661..53927)
                     /gene="ybiI"
                     /locus_tag="LAF92_000904"
     CDS             complement(53661..53927)
                     /gene="ybiI"
                     /locus_tag="LAF92_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBI9789957.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(54001..54678)
                     /gene="ybiX"
                     /locus_tag="LAF92_000905"
     CDS             complement(54001..54678)
                     /gene="ybiX"
                     /locus_tag="LAF92_000905"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBI9789958.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(54720..>55003)
                     /locus_tag="LAF92_000906"
     CDS             complement(54720..>55003)
                     /locus_tag="LAF92_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBI9789959.1"
                     /translation="QYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKL
                     GYRVNRNLDFQLNVYNLFDTDYVASINKSGYRYHPGEPRTFLLTANMHF"
BASE COUNT        12992 a        14016 c        14677 g        13318 t
ORIGIN      
        1 gccagtgcgg caccgctggc aaggcttaga gtggcagtca gaaataatgt ggccagtttt
       61 gtcattttca taggatgctc ctgttatggt cgttatgtcg gataacctct tccaacagtg
      121 catttgcagg tgaatataag gcattggttt aagatttcag ccaggttatg aaacgcagca
      181 gagaatcttg aaataattaa caaacaaagg agttacagtt agaaattgta ggagagatct
      241 cgtttttcgc gacaatctgg cgtttttctt gctaattcca ggattaatcc gttcatagtg
      301 taaaaccccg tttacacatt ctgacggaag atatagattg gaagtattgc attcactaag
      361 ataagtatgg caacactgga acagacatga attatcagaa cgacgattta cgcatcaaag
      421 aaatcaaaga gttacttcct cctgtcgcat tgctggaaaa attccccgct actgaaaatg
      481 ccgcgaatac ggttgcccat gcccgaaaag cgatccataa gatcctgaaa ggtaatgatg
      541 atcgcctgtt ggttgtgatt ggcccatgct caattcatga tcctgtcgcg gcaaaagagt
      601 atgccactcg cttgctggcg ctgcgtgaag agctgaaaga tgagctggaa atcgtaatgc
      661 gcgtctattt tgaaaagccg cgtaccacgg tgggctggaa agggctgatt aacgatccgc
      721 atatggataa tagcttccag atcaacgacg gtctgcgtat agcccgtaaa ttgctgcttg
      781 atattaacga cagcggtctg ccagcggcag gtgagtttct cgatatgatc accccacaat
      841 atctcgctga cctgatgagc tggggcgcaa ttggcgcacg taccaccgaa tcgcaggtgc
      901 accgcgaact ggcatcaggg ctttcttgtc cggtcggctt caaaaatggc accgacggta
      961 cgattaaagt ggctatcgat gccattaatg ccgccggtgc gccgcactgc ttcctgtccg
     1021 taacgaaatg ggggcattcg gcgattgtga ataccagcgg taacggcgat tgccatatca
     1081 ttctgcgcgg cggtaaagag cctaactaca gcgcgaagca cgttgctgaa gtgaaagaag
     1141 ggctgaacaa agcaggcctg ccagcacagg tgatgatcga tttcagccat gctaactcgt
     1201 ccaaacaatt caaaaagcag atggatgttt gtgctgacgt ttgccagcag attgccggtg
     1261 gcgaaaaggc cattattggc gtgatggtgg aaagccatct ggtggaaggc aatcagagcc
     1321 tcgagagcgg ggagccgctg gcctacggta agagcatcac cgatgcctgc atcggctggg
     1381 aagataccga tgctctgtta cgtcaactgg cgaatgcagt aaaagcgcgt cgcgggtaag
     1441 gtttaattgt cggatgcgcc gtcagagtgg cgtatccgat gaatcaccac aggcctgata
     1501 agtcgcgcag cgtcgcatca ggcaatgtgc tccattgtta gcaacaaaaa tgccgactca
     1561 cttgcagtcg gctttctcat tttaaacgaa tgacgtttac ttcgctttac cctggtttgc
     1621 aaccgccgct gctttcgctg cgatctcgtc agcattaccc agataatagc gtttcagcgg
     1681 tttgaaattc tcgtcgaact catacaccag cggcacgcca gtcgggatat taagctcaag
     1741 aatctcttct tcgctcatgt tatcaagata tttcaccagc gcacgtaaag agttaccgtg
     1801 tgcagcgatg atcacgcgct caccgctctt catacgcggc agaatagttt cattccagta
     1861 agggatcacg cggtcaatgg tcagcgccag gctttccgtc agcggcagtt ctttttcgct
     1921 cagtttcgcg taacgcggat cgtgacccgg ataacgctca tcatctttag tcagttccgg
     1981 cggagtcact gcaaaaccac gacgccactg tttcacctgc tcgtcgccat acttttcagc
     2041 agtttccgct ttgttcagac cctgcaacgc accgtagtga cgttcgttca gtttccagga
     2101 tttctcaacg ggcagccatg cctgatccag ttcgtccagc acattccaca gggtatggat
     2161 agcgcgtttc agcacagagg tgtaagcaaa gtcaaagctg taaccttcct ctttcagcag
     2221 cttacctgct gcttttgctt cgcttacgcc tttctcagac agatccacgt cgtaccaacc
     2281 ggtgaaacgg ttttctttgt tccactgact ttcgccatga cgaaccagaa ccagcttagt
     2341 tacagccata tacttactcc tcaaatcatt ttttaatgat aataattctc attatattgc
     2401 cgtgacgaag caacagcaat gcttacgcat aaccatagcg aaaatagtgt cgcagtgtaa
     2461 ggttgttgtg aatattgagt tgcaaatatg tcggtgtttg ctggtgattt gagcaatata
     2521 aggataaagc cctcattacg agggcttcag gtgctactca gcaataaact gatattccgt
     2581 caggctggaa tactcttcgc caggacgcag gaagcagtcc ggttgcggcc attcagggtg
     2641 gtttgggctg tccggcagaa actcgctttc cagtgccagg ccttgccagt cggcgtaagg
     2701 ttcagttcct cttgatggcg tgccgccgag gaagttgccg gagtagaatt gcagagccgg
     2761 agcggtggtg tagaccttca gctgcaattt ttcatctgct gaccagacat gcgccgccac
     2821 tttcttgcca tcgcctttgg cctgtaacaa gaatgcgtga tcgtaacctt tcactttgcg
     2881 ctgatcgtcg tcggcaagaa actcactggc gatgattttg gcgctacgga aatcaaaaga
     2941 cgttccggcg acagatttca ggccgtcgtg cggaatgccg ccttcatcaa ccggcagata
     3001 ttcgtccgcc agaatctgca acttgtgatt gcgcacgtca gactgctcgc cgtcaagatt
     3061 gaaatagacg tgattagtca tattcaccgg gcaaggttta tcaactgtgg cgcgataagt
     3121 aatagagata cggttatcgt cggtcagacg atattgcacc gtcgcgccga gattacccgg
     3181 gaagccctga tcgccatcat ctgaactcag ggcaaacagc acctgacgat cgttctggtt
     3241 cacaatctgc cagcgacgtt tgtcgaaccc ttccgggccg ccgtgcagct ggttaacgcc
     3301 ctgacttggc gaaagcgtca cggtttcacc gtcaaaggta taacggctat tggcgatgcg
     3361 gttggcataa cgaccaatag aggcccccag aaacgcagcc tgatcctgat agcattccgg
     3421 gctggcacag ccgagcagcg cctcgcggac gctgccatcg gaaagcggaa tacgagctga
     3481 aagtaaagtc gcaccccagt ccatcagcgt gactaccatc cctgcgttgt tacgcaaagt
     3541 taacagtcgg tacggctgac catcgggtgc cagtgcggga gtttcgttca gcactgtcct
     3601 gctccttgtg atggtttaca aacgtaaaaa gtctctttaa tacctgtttt tgcttcatat
     3661 tgttcagcga cagcttgctg tacggcaggc accagctctt ccgggatcag cgcgacgata
     3721 cagccgccaa atccgccgcc ggtcatgcgt acgccacctt tgtcgccaat cacagctttg
     3781 acgatttcta ccagagtgtc aatttgcggc acggtgattt cgaaatcatc gcgcatagag
     3841 gcatgagatt ccgccatcaa ctcgcccata cgtttcaggt cgccttgctc cagcgcgctg
     3901 gcagcttcaa cggtgcgggc gttttcagtc aggatatgcc gcacgcgttt cgccacgatt
     3961 gggtccagct catgtgcaac agcgttgaac tcttcaatgg tgacatcgcg cagagctggc
     4021 tgctggaaga aacgcgcacc ggtttcgcac tgttcacgac gggtgttgta ttcgctgcca
     4081 accagggtac gtttgaagtt actgttgatg atgacgacag ccacaccttt cggcatggaa
     4141 actgctttag tccccagtga gcggcagtca atcagcaagg catgatcttt cttgccgagt
     4201 gcggaaatta gctgatccat gatcccgcag ttacagccaa caaactggtt ttctgcttcc
     4261 tgaccgttaa gcgcgatttg tgcgccgtcc agcggcagat gataaagctg ctgcaatacg
     4321 gttccgaccg cgacttccag tgaagcggaa gaacttaacc cggcaccctg cggcacattg
     4381 ccgctgatca ccatgtccac gccgccgaag ctgttgttac gcagttgcag atgtttcacc
     4441 acgccacgaa cgtagttcgc ccattgatag ttttcatgcg cgacaatggg cgcatcgagg
     4501 gaaaactcgt cgagctgatt ttcataatcg gctgccatta cgcgaacttt acggtcatcg
     4561 cgtggcgcac agctgatcac ggtttgataa tcaatcgcgc agggcagaac gaaaccgtcg
     4621 ttgtagtcgg tgtgttcacc aatcaaattc acgcggccag gcgcctgaat ggtgtgagtg
     4681 gcagggtagc caaatgcgtt ggcaaacaga gattgtgttt tttctttcag actcatttct
     4741 tacactccgg attcgcgaaa atggatatcg ctgactgcgc gcaaacgctc tgctgcctgt
     4801 tctgcggtca ggtctcgctg ggtctctgcc agcatttcat aaccaaccat aaatttacgt
     4861 acggtggcgg agcgcagcag aggcggataa aagtgcgcgt gcagctgcca gtgttgattc
     4921 tcttcgccat taaatggtgc gccgtgccag cccatagagt aggggaagga gcactggaag
     4981 aggttgtcat aacgactggt cagctttttc aacgccagcg ccaaatcgct gcgctgggcg
     5041 tcggtcaaat cggtgatccg caaaacgtgg gctttgggca gcagtagcgt ttcgaacggc
     5101 caggcagccc agtaaggcac aacggctaac cagtgttcgg tttcgacaac ggtacggcta
     5161 ccgtctgcca gctcgcgctg aacataatcc accagcattg gtgatttctg ttcggcaaaa
     5221 tattcttttt gcaggcggtc ttcgcgctca gcttcgttag gcaggaagct atttgcccaa
     5281 atctgtccgt gcggatgcgg gttagagcag cccatcgccg cacctttgtt ttcaaagacc
     5341 tgcacccacg ggtacgtttt tcccagctct gcggtttgct cctgccaggt tttgacgatt
     5401 tccgtcaatg ccgcaacgct cagttctggc aacgttttac tgtgatccgg tgaaaagcag
     5461 atcacccggc tggtaccgcg cgcgctctgg caacgcatca aaggatcgtt gctttctggc
     5521 gcatctggcg tgtcagacat caaggccgca aagtcattag tgaaaacgta agttctggtg
     5581 taatcggggt ttttatcgcc tgtcacccgc acattacctg cgcagaggaa gcaatctgga
     5641 tcgtgcgcag gtaacacctg tttggctggc gtttcctgcg ccccctgcca ggggcgctta
     5701 gcgcggtgcg gtgaaaccag aatccattgc ccggtgagcg ggttgtagcg gcgatgtgga
     5761 tgatcaacgg gattaaattg cgtcatggtc gttccttaat cgggatatcc ctgtggatgg
     5821 cgtgactgcc agtgccaggt gtcctgcgcc atttcatcga gtgtgcgcgt tacgcgccag
     5881 ttcagttcac ggtcggcttt gctggcgtcc gcccagtagg ccggaaggtc gccttcgcga
     5941 cgcggtgcaa aatgatagtt aaccggtttg ccgcaggctt tgctgaaggc attaaccacg
     6001 tccagcacgc tgctgcctac gccagcaccg aggttgtaga tgtgtacgcc tggcttgttc
     6061 gccagttttt ccatcgccac gacgtgaccg tccgccagat ccattacgtg gatgtaatcg
     6121 cgtacgccag taccatcttc ggtcggataa tcgttaccaa aaatcgccag cgagtcgcga
     6181 cggcctacag caacctgggc gatgtatggc atcaggttat tcggaatgcc ttgcggatct
     6241 tcgcccatat cgcccgacgg atgcgcgcca accgggttga agtagcgcag cagggcaatg
     6301 ctccagtccg gctgggcttt ttgcaggtcg gtgaggatct gttccaccat caatttgctt
     6361 ttgccataag ggctttgcgg tgtgccggtc gggaagcttt caacgtatgg aattttgggc
     6421 tgatcgccat aaacggtggc ggaggagcta aaaataaagt ttttgacgtt agcggcgcgc
     6481 atggcgctaa tcaggcgcag agtgccgttg acattgttgt cgtaatattc cagcggtttt
     6541 tgtaccgatt cgccaacggc tttcagcccg gcgaagtgga tcacggtgtc gatagcgtga
     6601 tcgtgcagga tctcggtcat caacgcttcg ttacgaatat cgccttcaac aaacgttgga
     6661 tgtttgccgc ctaaacgctc gataacaggc agtacgctgc gcttactgtt acagaggtta
     6721 tcaagaatga tgacatcatg accgttttgc agtaattgca cgcaggtatg acttccaatg
     6781 taaccgctac caccggtaac cagaactctc ataattcgct ccattaggct tatggtatga
     6841 aataaccata gcataacaaa gatgcgaaaa gtgtgacatg gaataaatta gtggaatcgt
     6901 ttacacaaga atttagccgt tttttatgcg cgattaagtg attataaaac agagggttta
     6961 tgaatgattg cgctttttat ctgaaaaaag aagcggtttc atgcctgcat gcgtcgaacc
     7021 gttggccgga gagggcgctt aggccgcctc cggcaaggtc agcactaccg actcaatata
     7081 tttttgtcag cacatagcga tagagtccac cgtccggcac gaactcaagg cgatgggtaa
     7141 tacaggcagg cgcatcttca gcgtggtgcg aaacaaacaa caattgcgtt tcaccttcgc
     7201 taatcagcac atcaacaaaa cggcggataa gctggcgatt gagcggatca agcccctgta
     7261 gtggttcatc gagaataagc aacgtcggat gtttcaccag tgcgcggacg atcagcgcca
     7321 gacgctgctg tccccaggaa agactatgga acggagcgtc agccgtacgt ttatcaatgc
     7381 cgagaatatc cagccactgc tgcaccagtt tttgctggcg atccgaaacg gcttgataaa
     7441 tgccaatcga atcgaaatag ccagaaagaa tcacattacg cacggtagtg ctgacccggt
     7501 aatccagatg caaactactg ctgacgtaac cgatatgctt tttgatatcc cagatggttt
     7561 cgccgctgcc gcgacgtcgg ccgaaaagcg tcaaatcgtt gctgtaacct tgcggatgat
     7621 cgccagtaat caggcttaat aacgtcgatt ttcccgcacc atttggcccg acaatttgcc
     7681 agtgctcgcc aggattcacc tgccagctaa ggttattaag aatggggcga tcgttataag
     7741 aaaccacgcc attattcagc acaatgcgcg gttcgttggt gggtaaggcg tgacgtgctg
     7801 aaggttcatc cggctccggc agttgcacac cttcaagctg ttcgctatgc gccagttgcg
     7861 cgacgagtgc ttgctggagc agttcctctt tagcgcccgt ttccgctaac gtacaatccg
     7921 ccagcacgcc agcaaactgg acaaattccg ggatctcatc gaagcgattg agcaccagta
     7981 ccagagtaat accggactga tgtaacgagg cgagtcgctc agccagctgc tgacgtgagg
     8041 caacatccag gccatcgaac ggctcatcaa gaatcaacaa gtcaggctcc gacatcagcg
     8101 cctgacacag cagggttttt cgcgtctcgc cagtggaaag gtatttaaag cgtcggtcga
     8161 ggagggcggt aataccgaac tgctgcgcca gttgcatgca acgcggtgca tccttaactt
     8221 catcctgaat gatctcagcc gtagtgcgtc cggtgtcatc ttcgccaggg ccgagcatat
     8281 cggtgttatt ccgctgccat tcgtcgctga cgagcttttg caattgctcg aaggagagac
     8341 gagtgatgtg ggaaaactgg ctttgccgtt cacctttcaa aagcggaagt tcccccgcca
     8401 gcgcacgggc cagcgccgac tttccacttc cattcgaacc gacaaacgcc caactatcac
     8461 ccgcgtttaa cgttagctga ggcaattgca gcgtttttgt gtcgctaaga cgaaacgtgc
     8521 cttgcaaaat ttgcaacgat gacattttat atcccatttt gtgcagcgat tactgatagg
     8581 gatacgtgtt tcataacaaa ttgtcaacac gcttagcata gcgtggcgat aatcacgctg
     8641 tcggcattaa agtaggccgt gacattctgt ccttgctgaa gagaagtcgc ttcattaacc
     8701 ggcactgtgg cgcacagtgt ttgcccgtcg ggtagcgcca ttaatacttc gcactgctct
     8761 gcgccgcgct caatatgact aataataccc ggtaattggt tgtcggcgtt ttgtgcgacc
     8821 gcttcgtcct gagtaatgcc tacccacggc gcttttagca atatcaacac ttctttgcct
     8881 tcatccagcc ccagacgtgc gccgctttgt gcggtaattg cgactttcag gcgtgttttt
     8941 ccgtcagcca gtaagacatc aacatgctgt tgaacgtcat catgatcgcg ggcggtgatg
     9001 gtgccgaacc actggttacg ggcgctggtt tgcagtgaaa aacgtgagat cgcggccagc
     9061 aggctgttca gcggcagggc gtcatcgtca cttaacacat caaaggcttt ttgctggatt
     9121 tgcgccagta agtcatagag ctgaatcagt cgctgaccat agcgggtcag tactgcgccg
     9181 ccgccacctt taccgcctgt tgcgcgctcg accagaatat gctcacttaa ctgattcatc
     9241 tcgttaatgg catcccaggc gcttttatag ctgataccgg catctttcgc tccctggcta
     9301 atggaaccgg aaagcgcaat gtgttttagt agcgaaatgc ggcgcgggtc ggcgaataat
     9361 ttttgttgga gcttaagggt gagaaggatt tcggcctgca taacaatgtc ctggcaaaag
     9421 tcttattgtg acggaaaacg aacgccacgc aaagctgacc gcacaaaagg ggagtgcttt
     9481 tctgtgctta gcggttagaa tagtctcatg actatatctg gagttgacca tgttagagtt
     9541 attaaaaagt ctggtattcg ccgtaatcat ggtacctgtc gtgatggcca tcatcctggg
     9601 tctgatttac ggtcttggtg aagtattcaa catcttttct ggtgttggta aaaaagacca
     9661 gcccggacaa aatcattgat tccctgaatg cccgcttagt cgggcatttt ctttttctca
     9721 acttcctgct tttcctgccg atattttttc ttatctacct cacaaaggtt agtaataact
     9781 gctgggaaaa ttccgagtta gtcgttatat tgtcgcctac ataacgttac attaaggggt
     9841 taccaatggc tcgtaaatgg ttgaacttgt ttgccggggc ggcactctct ttcgctgttg
     9901 ctggcaatgc tctggcagat gaagggaaaa tcacggtgtt cgccgccgca tcactgacta
     9961 acgcaatgca ggacattgct acgcagtata aaaaagagaa aggcgtggat gtggtttctt
    10021 ctttcgcttc gtcatctact ctcgcccgtc agattgaagc gggtgcgcct gcggatctgt
    10081 ttatttctgc cgatcagaaa tggatggatt atgcagttga taaaaaagcg atcgatacag
    10141 ctacgcgtca gacactgctc ggcaatagcc tggtcgttgt agcaccgaaa gccagcgagc
    10201 agaaagattt catcatcgac agcaaaacta actggacttc gctgctgaat ggcggtcgcc
    10261 tcgcggttgg cgatccggaa catgtccctg ctggcattta tgcaaaagaa gcactgcaaa
    10321 aactgggcgc atgggatacg ctctccccga aactggctcc ggcggaagat gttcgtggcg
    10381 cgctggcgtt ggtcgaacgc aatgaagcgc cgttgggcat tgtttacggt tctgacgcag
    10441 ttgtcagcaa aggggtaaaa gtggttgcca ccttcccgga agattcacat aaaaaagtgg
    10501 aatatccggt tgctgttgtg gaagggcata acaatgcgac agtgaaagca ttttatgatt
    10561 atctgaaggg accgcaggca gcggaaatct ttaaacgtta cggatttaca accaagtaat
    10621 gatactgacc gatccagaat ggcaggcagt tttattaagc ctgaaagttt cttccctggc
    10681 tgtgctgttt agcctgccgt ttgggatctt ttttgcctgg ttactggtgc gttgcacgtt
    10741 tccgggcaaa gctctgctcg acagcgtact gcatctaccg ctggtgttac cgcccgtggt
    10801 cgtcggttac ttattattag tttcgatggg acggcgcgga tttatcggtg aacgtctgta
    10861 tgactggttt ggtattacct tcgcctttag ctggcgcggc gcggttctcg ctgccgccgt
    10921 catgtcgttt ccgctgatgg tgcgggcaat tcgtctggcg ctggaagggg ttgatgtcaa
    10981 actcgaacag gccgcaagaa cactgggggc cgggcgctgg cgcgttttct ttactatcac
    11041 gttaccgctg accttaccgg gaattattgt tggtacggta ctggcttttg ctcgttctct
    11101 cggtgagttt ggtgcaacca tcacctttgt gtcgaacatt cctggtgaaa cgcgaaccat
    11161 tccttctgcc atgtataccc tgatccagac ccccggcggg gaaagtggcg cggcgagact
    11221 gtgcattatt tctattgcgc tggcgatgat ctccctgttg atttcagaat ggctggccag
    11281 aatcagccgt gaacgggcgg ggcgctaatc atgctggaac tgaatttttc ccagaccttg
    11341 ggcaaccatt gcctgaccat taatgaaacg ctgcccgcca atggcatcac tgctatcttt
    11401 ggcgtctccg gtgccggaaa aacttcgctg attaacgcca tcagtggact gacgcgcccg
    11461 caaaaaggga ggattgtcct caatggtcgg gtactaaatg atgccgaaaa aggtatctgc
    11521 ctgacgccag aaaagcgtcg cgttggctat gtttttcagg atgcgcggct gtttccgcat
    11581 tacaaagtgc gtggcaatct gcgctacggc atggcgaaaa gcatggtcga tcagttcgat
    11641 aagctggtgg cgcttttagg cattgaaccg ttgcttgacc gtttaccagg cagcctgtcc
    11701 ggaggcgaaa aacagcgcgt ggcgattggt cgggctttgc tgacggctcc ggaattgctg
    11761 ttgctggatg aaccgctggc gtcactggat attccgcgta aacgcgaact gttgccttat
    11821 ctgcaacgtc tgacgcggga aatcaacatt ccgatgttgt atgtcagcca ttcgctggat
    11881 gagatcctcc atctggcaga cagagtgatg gtactggaaa acggtcaggt gaaagccttt
    11941 ggcgcgctgg aagaagtctg gggtagtagc gtgatgaatc cgtggctgcc gaaagagcaa
    12001 caaagtagca ttctgaaagt gacggtactt gagcatcatc cgcattacgc gatgactgcg
    12061 ctggcgctgg gcgatcagca tttgtgggtc aataagctgg acgaaccgct gcaagctgcg
    12121 ctacgtatcc gcattcaggc ttccgatgtt tctctggttt tacaaccgcc gcagcaaacc
    12181 agcattcgta acgtattgcg ggcaaaagtt gttaatagtt atgacgacaa cggccaggtg
    12241 gaagtggagc tggaagtcgg cggtaaaacg ctgtgggcgc gtatcagccc gtgggccagg
    12301 gatgaactgg cgatcaaacc tggcctgtgg ctgtacgcgc aaattaaaag tgtgtcgata
    12361 accgcctgat taaatcaggt ggctataaat gaactgggca atgctgtcgg tggtgttatc
    12421 accaatcaca atgttggcgc gcgcttttac cgcgtcatcg gcgttgccca tcgccacgcc
    12481 tgtaccagcg gcttccagca tactgatatc attaaagtta tcgccgaatg ccacgacgtt
    12541 ttccatcgac caaccttgcg cctcaaccca tttcgtcaaa cgtttacctt tgctgttgcc
    12601 gccgcgtgca atatcaacct gatcgtgcca ggaccattca cactccagtc ccagttcatg
    12661 ttcgacatgc ttaccaaaat gctgcaattg cggcaggtca tcgtgcgtca gggcgaactt
    12721 ccatacggcg ttaacttgtt gcgccgtttc agccagagaa gcgacttgtg tgaaagtcgg
    12781 acgctgttcc ggcggcaggg tttgcgccca gttagacgtg cgaatgacat gcccggtcgg
    12841 gtgctcatag accattgcat catcgacata catcagaccg tgaatgtggt gttcattcag
    12901 catctcaatg agttgcaggg ctttattaac gggcattggg tccgcttcca gcacggtttt
    12961 tgcatgataa tcatacaaat aggtgccatt acagcaaata gcaggtgtat ccagcgccag
    13021 cgcctgataa aaaggatgaa tagcgacgtg atggcgacct gtgacgatga ttaattgata
    13081 gcctgcttcg cgagcgcggg ccagggcttc tatcgatgaa ggaagcaggg tctttttcgg
    13141 ggtcaataag gtgccgtcta agtcgagagc aatcacgcgt gtggtcatgg cgtattccag
    13201 attaaggtta agaattttcg tctgcgcgaa tggtacaccg ataccactat cggacaaaat
    13261 tctgcgtttt aattcagcat tcaccgccaa aagcgactaa ttttagctgt tacagtcagt
    13321 tgctaaatgc aaaggagcat tcatgaagca aacagtttat atcgccagcc ctgagagcca
    13381 gcaaattcac gtctggaacc tgaatcatga aggcgcactg acgctgacac aggttgtcga
    13441 tgtgccgggg caggtgcagc cgatggtggt cagcccggac aaacgttatc tctatgttgg
    13501 tgttcgccct gagtttcgcg tcctggcgta tcgtatcgcc ccggacgatg gcgcactgac
    13561 ctttgccgca gagtctgcgc tgccgggtag tccgacgcat atttccaccg atcaccaggg
    13621 gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg agcgtaacgc gtctggaaga
    13681 tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg gacggttgcc attccgccaa
    13741 tatctcaccg gacaaccgta cgctgtgggt tccggcatta aagcaggatc gcatttgcct
    13801 gtttacggtc agcgatgatg gtcatctcgt ggcgcaggac cctgcggaag tgaccaccgt
    13861 tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac gaacaatatg cgtattgcgt
    13921 caatgagtta aacagctcag tggatgtctg ggaactgaaa gatccgcacg gtaatatcga
    13981 atgtgtccag acgctggata tgatgccgga aaacttctcc gacacccgtt gggcggctga
    14041 tattcatatc accccggatg gtcgccattt atacgcctgc gaccgtaccg ccagcctgat
    14101 tactgttttc agcgtttcgg aagatggcag cgtgttgagt aaagaaggct tccagccaac
    14161 ggaaacccag ccgcgcggct tcaatgttga tcacagcggc aagtatctga ttgccgccgg
    14221 gcaaaaatct caccacatct cggtatacga aattgttggc gagcagggac tactgcatga
    14281 aaaaggccgt tatgcggtcg ggcagggacc aatgtgggtg gtggttaacg cacactaacc
    14341 gctgatttaa ccggcgcagt ctctcctgcg ccggtgtatt aacctatctc ctgtaacgca
    14401 tgtctctggc gttcgacgat attggtccac aaattgtctt tatcgtcagt ccacaaatta
    14461 atcagcaagg caaaaaagcg ttctgctgcc ggagaaagca cggcatcttt gcggcgaata
    14521 atccccaatg tccgacgtat aacgggttcc accagtggga taccaataat cgaagaatag
    14581 ggcgcatggg gcattgccag accaggaagc gccgaaatcc ccagtcctgc ctccactagt
    14641 cctaatgacg tcgaaagatg acgcacttcg taaaaccagt ccagcttcca gggcttgtcg
    14701 gctagctgtt gttctatcaa caatcggttg ccgctggagg aacgtacgcc aatcattttg
    14761 taacccacta actcctgcca ttctacaagc tgctttttgg ccaatggatg gtcacgccga
    14821 caggccagca cgaatggctc gttgaccagt ggggtaaaat caatggatga atttgtgacg
    14881 ttgttcatgt ttatgccaaa gtctgattcg ttacacagca ccgattccat gcaattattc
    14941 gtgccttgtt ccagaatccg cactttaata ttgggataca gctcattaaa tttaccgatt
    15001 gccagcggta aaaaataaaa tactgcggtc ggaatacacg ctaacgtcac cataccacga
    15061 tgataagcat tcatatcacg aatattaaaa agcgtttcat cgaatttctt tattaattcc
    15121 cgcgcctccg ggagcaacct ttttcctgct tttgttaacg taaccttgcg agttgtacgc
    15181 tcaaatagtt gaatatgtaa atcttcctcc atctttttta ttcttcgcgt taatgcaggt
    15241 tgcgtaatat tgagtaattt agcggcattg ttaaatgaac tggactctgc cagtatgaca
    15301 aatgccttca tacttgataa ttcatgcttc attattactc cggaaaatgg aagcgacgat
    15361 tttgggtggc tggccgttaa aaattttaac tgcatttagc caacttaaat taatgaaaaa
    15421 atgttattaa tcgttgagct aaagtcatta gagatgcttt gcccttaatg taaccatatc
    15481 gcaataagtt atgtttttaa attgagggca ttattatgaa aaaaataccc tgcgtgatga
    15541 tgcgaggtgg aacctcgagg ggcgcgttct tgttagcgga acatttaccc gaagatcaaa
    15601 cgcagcgcga taaaatattg atggcaatta tgggttccgg taacgatctg gaaattgacg
    15661 gtattggcgg cggtaatccg ctgaccagta aagtcgccat tattagccgt tccagcgatc
    15721 cgcgtgctga tgtcgattat ctgtttgctc aagttattgt ccatgagcaa cgtgtcgata
    15781 ccacgcctaa ttgtggcaat atgctgtctg gtgttggggc attcgccatt gaaaatggtt
    15841 tgattgcagc gacttcacca gttacccgcg tacgtatccg caacgtcaat actggtacgt
    15901 tcatcgaagc tgatgtgcaa acgccaaatg gtgttgtcga gtacgagggt agcgccagaa
    15961 ttgacggcgt accgggtact gccgcaccgg ttgcgctcac tttcctgaat gccgccggaa
    16021 ccaaaaccgg aaaagtcttc ccgactgata atcagattga ttattttgac gatgtcccgg
    16081 tgacctgtat cgatatggcg atgccagtcg tcattattcc ggctgaatat ctgggaaaaa
    16141 caggttatga attaccggcg gaactggatg ccgacaaagc attattagcc cgcattgaat
    16201 ctatccgtct acaagcgggt aaagcaatgg gcttaggtga tgtcagtaat atggttatcc
    16261 ctaaacctgt gcttatttct ccagcgcaga aaggcggggc aattaatgtg cgttatttta
    16321 tgccgcattc ttgccatcgc gcgctggcga taaccggtgc tattgctatt tccagtagtt
    16381 gtgcattgga aggcaccgtc acccgacaaa tcgtcccttc tgtaggatac ggcaatatca
    16441 atattgaaca ccccagtggt gcgctcgacg ttcatttaag taatgaaggt caggatgcca
    16501 cgacgttacg cgcatctgtt attcggacaa ccagaaaaat attttccggt aaagtttatc
    16561 ttccctgaaa aaattcgttg tcaggataag gacaatcaat aaaggacttc tgtatgagtc
    16621 atacagaaag aacaggattt taaatgaata agaaatcgtt atggaagcta attctgatat
    16681 tagcgatccc atgtattatt ggctttatgc cagctccggc aggattaagc gaactggcct
    16741 gggtgctttt tggtatttac ctggcggcca ttgtggggct ggttatcaag cctttcccgg
    16801 aacctgtcgt actgttaatt gccgttgctg cctcgatggt ggtggtcggt aacttatccg
    16861 acggtgcgtt taagaccacc gccgtattaa gcggttactc ttcaggtacc acctggctgg
    16921 tgttctcggc gtttacctta agcgccgcat ttgtgaccac cggcttaggt aaacgtattg
    16981 cctatttgct gattggtaaa attggtagca ctaccctggg tctgggttac gttacggtat
    17041 ttctcgatct ggtactggct ccggcaacac cgtctaacac cgcgcgtgcg ggcggcattg
    17101 tgttaccgat catcaacagc gtggcggtgg ctttggggtc cgaaccggaa aaaagtccgc
    17161 gtcgtgtcgg acattacctg atgatgtcca tttacatggt caccaaaacc accagctata
    17221 tgttctttac cgcaatggcg gggaacattc tggcgctgaa aatgatcaac gacattctgc
    17281 acctgcaaat tagctggggt ggatgggcgc tggccgccgg attgccgggc atcattatgc
    17341 tgctggtcac cccgctggtg atttacacca tgtatccgcc agaaattaag aaggtggata
    17401 acaaaaccat cgccaaagcg ggccttgccg aactgggacc gatgaaaatc cgcgaaaaaa
    17461 tgctgctcgg tgtctttgtg ctggcgctac tgggctggat tttcagtaag tctctggggg
    17521 ttgatgaatc caccgtggca atcgttgtta tggcgaccat gctgctgctg ggtatcgtta
    17581 cctgggaaga cgtggttaaa aataaaggcg gctggaatac cttaatctgg tacggcggta
    17641 ttatcggctt aagctcctta ttatcgaaag ttaaattctt cgaatggtta gctgaagtct
    17701 ttaaaaataa cctggcattt gatggtcacg gtaacgttgc tttcttcgtt attattttcc
    17761 tcagcattat cgtgcgttat ttcttcgctt ccggtagtgc ctatatcgtt gctatgttac
    17821 cggtatttgc catgctggcg aacgtctccg gcgcaccgtt aatgttaact gcgctggcac
    17881 tgttgttctc caactcctat ggcggcatgg ttactcacta tggcggcgcg gcaggtccgg
    17941 tcatctttgg cgtgggttat aacgatatta aatcctggtg gttggtcggt gcggtactga
    18001 cgatattaac cttcctggtg catatcaccc tcggcgtgtg gtggtggaat atgctgatcg
    18061 gctggaacat gctgtaaata tactcgtcat acttcaagtt gcatgtgctg cgtctgcgtt
    18121 cgctcacccc agtcacttac ttatgtaagc tcctggggat tcactcgctt gtcgccttcc
    18181 tgctactcga attatttaga gtatatccat ttattatctt tctgcgcact tcacggtgcg
    18241 cagatatcgg gagcatttga tgatcaagtt atctgaaaaa ggcgtgtttc tcgccagtaa
    18301 taacgaaata attgccgaag aacatttcac cggcgaaatt aaaaatgaag aagccaaaaa
    18361 aggcactatt gcctggtcta ttctctcttc gcataatacg tccggaaata tggataaact
    18421 taaaattaag tttgattcat tagcctctca cgatattacc tttgttggta ttgtacagac
    18481 cgctaaagcg tccggaatgg aacgtttccc gctgccgtat gtgctgacca actgccataa
    18541 ctcactctgc gccgtcggcg gcaccattaa cggtgatgac catgtttttg gtttatcggc
    18601 ggcccagcgt tatggcggta tttttgtgcc tccgcacatt gcggtcatcc atcaatatat
    18661 gcgtgagatg atggcaggcg gcggcaaaat gatcctcggg tcagacagcc acacccgtta
    18721 cggtgcatta gggacaatgg cagtcggtga gggtggcggt gagttggtaa aacagctgct
    18781 taatgacacc tgggatatcg actatccggg cgtggttgcg gtgcatctga ccggaaaacc
    18841 tgcgccgtat gtggggccac aggatgtggc gctggctatc attagcgcgg tgttcaaaaa
    18901 cggttacgtc aaaaacaaag tcatggagtt cgttggaccg ggcgttagcg cgctctctac
    18961 cgatttccgt aacagcgttg acgtgatgac cacggaaacc acctgtttaa gctccgtctg
    19021 gcaaactgat gaagaagtgc ataactggct ggcgctgcac ggtcgcggcc aggattactg
    19081 ccagcttaac cctcaaccga tggcgtacta cgatggctgc atcagcgttg atttaagcgc
    19141 catcaaacca atgattgcgc tgccgttcca cccgagcaac gtgtataaaa tcgacacact
    19201 gaaccagaac ctgaccgaca ttctgcgtga gattgaaatt gagtccgaac gcgtggcgca
    19261 cggtaaagcc aaactctcgc tgctggataa agtggaaaat ggtcgcctga aagtgcagca
    19321 ggggattatc gcgggctgtt ctggcggtaa ctacgaaaac gtcatcgcgg cggcgaatgc
    19381 actgcgcggt caatcctgtg gcaatgacac cttctcgctg gcagtttacc cgtcatcaca
    19441 gccggtgttt atggatctcg ccaaaaaagg tgtggtagca gatttgattg gcgcaggcgc
    19501 aatcatcaga accgcgttct gcggcccatg ctttggcgcg ggcgatacgc caatcaacaa
    19561 cggtttgagt attcgccaca ccacgcgtaa cttcccgaac cgcgaaggct ctaagccagc
    19621 taatgggcag atgtcagcgg tggcgttgat ggacgctcgt tctatcgctg cgactgcggc
    19681 aaacggtggc tatttaacct ctgccagcga acttgattgc tgggacaacg tgccggagta
    19741 cgccttcgat gtaacgccgt ataaaaaccg tgtttatcag ggctttgtga aaggggcaac
    19801 tcagcaaccg ctgatttacg gaccgaacat taaagactgg ccggaattgg gtgcgctgac
    19861 tgacaatatc gtcctgaaag tgtgctcgaa gatcctcgac gaagtgacca ccaccgacga
    19921 actgattcct tccggtgaaa cctcttctta tcgttcaaat ccgattggtc tggcggagtt
    19981 taccctgtca cgccgcgatc ccggttatgt tggcagaagt aaagcgactg ctgagctgga
    20041 aaatcagcgt ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca
    20101 gattgctggt caggagcata ttgatccgct gcaaactgaa attggcagca tggtatatgc
    20161 ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg
    20221 cggtctggcg aatattgccg aagagtacgc gactaaacgc taccgttcta acgtcatcaa
    20281 ctgggggatg ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat
    20341 ttacatccct ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt
    20401 gatccatgaa gatgcgccgg taacggaaat tacgctctat atggaaagtc tgactgctga
    20461 agagcgcgag attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta
    20521 aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgcttgcgc
    20581 atcttatccg acctacgaat cgtatcgaat ctgtaggcca gataaggcat tttcgcagca
    20641 tccggcactt attgtcggat gcgatgcttg cgcatcttat ccgacctaca aatcgcatcg
    20701 aaccgtaggc cggataaggc gtttacgccg catccggcaa atagttaatt gctcttactt
    20761 cttcgcctct gcaaccactt tactacccac gccgcggtta ttgtattccc acatgcggtt
    20821 gtagttagtg tcattcagat tgcgctgtat ttcgtcgtta tcatctacgc tgccggtatt
    20881 acccgcaaac ggacgattag agatcaccgc atcggcccac ggtttagccg tgttaaaacc
    20941 ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg gtattggcat caacatccag
    21001 cgagcggccc agttgcgcca caccatcacc gaaagcattg aaacggctgt ttacggcgag
    21061 gaaaccgtag taaatgttgg acagcgtagc cggtgcaaac acatacgctt cttgctgagt
    21121 acgtgagttc accacgcgga attcggtgtt atcgaacacc actgcgccgc gaccagaaac
    21181 gatatccaca tccccttcaa tgtagctgtt ggtcaccagc gtacgcggct gacggttggt
    21241 ttccagacgg ttctgcacac cgctgttggt gacaaagaag gtgttctgac gaccgagaat
    21301 gttaacgttg ttaatctgta cctggtcacc atcagtacgc agtgccaccg ccggatggtt
    21361 acctgcatct acgctatcgc ccagcgtgtt ttcgatggtc agattttgca gttgcaggcc
    21421 attgttttgt gaccagaaga ccgcagagca gagaacaccg atactgtcgc tgcgtttgct
    21481 ctggcagcta tcgtacatat accacgctgg tttacctggc atatatttgc cgcgcgggtt
    21541 gacgtcgtga cgccagtcgg cagggctcat gccaccatca agggaaagcc caatcttcac
    21601 atcaatcggt ttttcacctg taccgtacag agtaattcca cccggagcgg cagggacata
    21661 taccgttccc tgatactcac caggcatcac ggcaatatac tggcgcttgt tggtacgctt
    21721 gataattgcc gcatctaccg ccgcctgaat cgtggtatgc gttacacctt gagtgcccgc
    21781 cgggccgaca acaaagtcag gttgcgcagg cagggtaatc ggggaaggat tccacgctgc
    21841 agcacctggt gtcagggatg caaaatagtg ttgagcatcg aaattctgcg cttcttttgc
    21901 cgacagaatc gggcgagaag aggtaccagg cgcggtttga tcagaaggac gttgatcggg
    21961 cggggttgag ctacaggcgg tcagcgtcac gccaaaagcc aatgccagcg ccagacggga
    22021 aactgaaaat gtgttcacag gttgctccgg gctatgaaat agaaaaatga atccgttgaa
    22081 gcctgctttt ttatactaac ttgagcgaaa cgggaaggta aaaagacaaa aagttgtttt
    22141 taataccttt aagtgatacc agatggcatt gcgccatctg gcagagtgat taactaaaca
    22201 tcgcagtaat cgaggcgctt gccagagagt ggaaatgaac gttaaacccg accatcgcgc
    22261 cgctggcacc ttcatcgaca tcaatacgtt ctatatccag cgcgtgaacg gtaaaaatgt
    22321 agcgatgagt ttcgcctttc ggcggtgctg cgccatcgta cccggtttta ccaaagtcgg
    22381 tacgcgtctg caaaacgccg tctggcattg ctaccagacc agagccaaac ccttgcggta
    22441 atacgcgggt atcagcgggt aagttaacaa ctacccagtg ccaccagccg gagccggttg
    22501 gcgcatccgg gtcgtagcag gtgacaacaa aacttttcgt tcccgcagga acatcatccc
    22561 acgccagatg cggtgaaata ttatcgccat cgtaacccat gccgttaaag acatgacgat
    22621 gcggcaattt atcgccatcg cgcagatcgt tactgatgag tttcatgaac cctcctttct
    22681 tgtttgcaga aagtgtagcc agaaaccctc acgcggactt ctcgttattg gcaaaaaaat
    22741 gtttcatcct gtaccgcgcg gttaaccgct gcggtcagac gctgcaactg ttgcgggaga
    22801 ataatatagg gcggcatcag gtaaatcagt ttgccaaaag gccggatcca gacaccctgt
    22861 tcgacaaaga atttttgcag cgccgccata ttcaccggat gagtggtttc gactacgccg
    22921 attgccccca gcacgcgcac atcggcaacc atttcggcat cacgggcggg ggcaagttgc
    22981 tcgcgcagct gtacttcaat agccgccacc tgttgctgcc agtcgccaga ttcgagaatc
    23041 gccaggctgg cgtttgctgc cgcgcaggcc agcggattgc ccataaaagt tggcccatgc
    23101 ataaagcaac cggcttcgcc gttactgatg gtttccgcaa cctcgcgcgt ggtgagcgtg
    23161 gcggaaaggg tcattgtgcc gccggttaag gctttaccga ggcacaaaat gtccggcgcg
    23221 atttctgcat gttcacaggc aaacagtttg ccggtacgac caaatccggt ggcgatctcg
    23281 tcggcaatca gcaagatacc ttcgcgatcg cacattttgc ggattcgttt taaccattcc
    23341 ggatggtaca tgcgcatccc gcctgcgccc tggacaatcg gttcaatgat caccgccgcg
    23401 atttcatgac gatgcgccgc catcaggcgg gcaaagccaa ccatatcacg ttcatcccat
    23461 tcaccatcca tgcggctttg cggggcggga gcaaacaagt tttctggcag atagcctttc
    23521 cacaggctgt gcattgagtt gtccggatcg cacaccgaca tcgcgccaaa ggtatcgcca
    23581 tgataaccat tgcggaaggt cagaaaacgc tggcgcgctt cgcctttggc ctgccagtac
    23641 tgcaacgcca ttttcatcgc cacttccacc gctacggagc cggagtccgc gagaaaaacg
    23701 cattccagcg gttgcggcgt cattgccacc agtttgcggc atagctcgat ggcgggcgca
    23761 tgggtgatac cgccaaacat cacatgcgac atagcatcaa tttgcgactt catcgccgca
    23821 ttaagctgcg ggtgattata gccgtggatc gccgcccacc aggacgacat accgtcaacc
    23881 aggcgtctgc cgtcagacaa aattagctcg caaccttcgg cgctcgcgac cggataaacc
    23941 ggcagagggg aggtcatgga tgtgtatggg tgccagatat ggcgttggtc aaaggcaaga
    24001 tcgtccgttg tcataatcga cttgtaaacc aaattgaaaa gatttaggtt tacaagtcta
    24061 caccgaatta acaacaaaaa acacgttttg gagaagcccc atggctcacc gcccacgctg
    24121 gacattgtcg caagtcacag aattatttga aaaaccgttg ctggatctgc tgtttgaagc
    24181 gcagcaggtg catcgacagc atttcgatcc tcgtcaggtg caggtcagca ctttgctgtc
    24241 gattaagacc ggagcttgtc cggaagattg caaatactgc ccgcaaagct cgcgctacaa
    24301 aaccgggctg gaagccgagc ggttgatgga agttgaacag gtgctggagt cggcgcgcaa
    24361 agcgaaagct gcgggatcga cgcgtttctg tatgggcgcg gcgtggaaga atccccacga
    24421 acgcgatatg ccgtacctgg aacaaatggt gcagggggta aaagcgatgg ggctggaggc
    24481 gtgtatgacg ctgggcacgt tgagtgaatc tcaggcgcag cgcctcgcga acgccgggct
    24541 ggattactac aaccacaacc tggacacctc gccggagttt tacggcaata tcatcaccac
    24601 ccgcacttat caggaacgcc tcgatacgct ggaaaaagtg cgcgatgccg ggatcaaagt
    24661 ctgttctggc ggcattgtgg gcttaggcga aacggtaaaa gatcgcgccg gattattgct
    24721 acaactggca aacctgccga cgccgccgga aagcgtacca atcaacatgc tggtgaaggt
    24781 gaaaggcacg ccgctggcgg ataacgatga tgtcgatgcc tttgatttta ttcgcaccat
    24841 tgcggtcgcg cggatcatga tgccgacctc ttacgtgcgc ctttctgccg gacgcgagca
    24901 gatgaacgaa cagactcagg cgatgtgctt tatggcaggc gcaaactcga ttttctacgg
    24961 ttgcaaactg ctaaccacgc cgaatccgga agaagataaa gacctgcaac tgtttcgcaa
    25021 actggggcta aattcgcagc aaactgccgt gctggcaggg gataacgaac aacagcagcg
    25081 tctggagcag gcactgatga ccccggacac tgacgaatat tacaacgcgg cagcactatg
    25141 agctggcagg agaaaatcaa cgcggcgctc gatgcgcggc gtgctgccga tgccctgcgt
    25201 cgccgttatc cagtggcgca aggagccgga cgctggctgg tggcggatga tcgccagtat
    25261 ctgaactttt ccagtaacga ttatttaggt ttaagccatc atccgcaaat tatccgtgcc
    25321 tggcagcagg gtgcggagca atttggcgtc ggtagcggcg gttccggtca cgtcagcggt
    25381 tatagcgttg cgcatcaggc gctggaagaa gaactggccg agtggctggg ctattcgcgg
    25441 gcactgctat ttatctctgg ttttgccgct aatcaggcgg tcattgccgc gatgatggca
    25501 aaagaggacc gcattgtcgc tgaccggctt agccatgcct cattgctgga ggctgccagt
    25561 ttaagcccgt cgcagcttcg ccgttttgtt cataacgatg tcactcatct ggcgcgactg
    25621 cttgcttccc cctgtccggg gcagcaactg gtggtgacag aaggcgtgtt cagcatggac
    25681 ggcgatattg cgccactggc ggaaatccag caggtaacgc aacagcacaa tggctggttg
    25741 atggtcgatg atgcccacgg cacgggcgtt atcggggagc aggggcgcgg cagctgctgg
    25801 ctgcaaaagg taaaaccaga attgctggtg gtgacttttg gcaaaggatt tggcgtcagt
    25861 ggggctgcgg tgctttgctc cagtacggtg gcggattatc tgctgcaatt cgcccgccac
    25921 cttatctaca gcaccagtat gccgcccgct caggcgcagg cattacgtgc gtcactggcg
    25981 gtcattcgcc gtgatgaggg ggatgcacgg cgcgaaaaac tggtgtcact cattgcgcgt
    26041 tttcgtgccg gagtacagga tttgccgttt acgctggctg attcatgcag cgccatccag
    26101 ccattgatcg tcggtgataa cagccgtgcg ttacaactgg cagaaaaact gcgccagcaa
    26161 ggctgctggg tcacggcgat tcgcccgcca accgtacccg ctggtactgc gcgactgcgc
    26221 ttaacgctaa ccgcagcgca tgaaatgcag gatatcgacc gtctgctgga ggtgctgcat
    26281 ggcaacggtt aataaacaag ccattgcagc ggcatttggt cgggcagccg cactctatga
    26341 gcaacacgca gatctacagc gccagagtgc tgacgtctta ctggcaatgc tcccacagcg
    26401 taaatacacc cacgtactgg acgcgggttg tggacctggc tggatgagcc gccactggcg
    26461 ggaacgtcac gcgcaggtga cggccttaga tctctcgccg ccaatgcttg ttcaggcacg
    26521 ccagaaggat gccgcagacc attatctggc gggagatatc gaatccctgc cgttagcgac
    26581 tgcgacgttc gatcttgcat ggagcaatct cgcagtgcag tggtgcggta atttatccac
    26641 ggcactccgc gagctgtatc gggtggtgcg ccccggaggc gtggtggcgt ttaccacgct
    26701 ggtgcaggga tcgttacccg aactgcatca ggcgtggcag gcggtggacg agcgtccgca
    26761 tgctaatcgc tttttaccgc cagatgaaat cgaacagtcg ctgaacggcg tgcattatca
    26821 acatcatatt cagcccatca cactatggtt tgatgatgcg ctcagtgcca tgcgttcgct
    26881 gaaaggcatc ggtgctacgc atcttcatga agggcgcgac ccgcgaatat taacgcgttc
    26941 gcaattgcag cgattgcaac tggcctggcc gcaacaacag gggcgatatc ctctgacgta
    27001 tcatcttttt ttaggagtga ttgctcgtga gtaaacgtta ttttgtcacc ggaacggata
    27061 ccgaagtggg gaaaaccgtc gccagttgtg cacttttaca agccgcgaag gcagtaggtt
    27121 atcggacggc aggttataaa ccggtcgcct ctggcagcga aaagaccccg gaaggcttac
    27181 gtaatagtga cgcgctggcg ttacagcaca acagcagcct gcagctggat tacgcaacgg
    27241 taaatcctta caccttcgca gaacccacct cgccgcacat catcagcgcg caagagggca
    27301 ggccgataga atcattggta atgagcgccg gattacgcgc gcttgaacaa caggctgact
    27361 gggtgttagt ggaaggtgct ggcggctggt ttacgccgct ttctgacact ttcacttttg
    27421 cagattgggt aacacaggaa caactgccgg tgatactggt agttggtgtg aaactcggct
    27481 gtattaatca cgcgatgttg actgcgcagg caatacaaca cgccggactg actctggcgg
    27541 gttgggtggc gaacgatgtt acgcctccgg gaaaacgtca cgctgaatat atgaccacgc
    27601 tcacccgcat gattcccgcg ccgctgctgg gagagatccc ctggcttgca gaaaatccag
    27661 aaaatgcggc aaccggaaag tacataaacc ttgccttgtt gtagctatcc tgtatttggt
    27721 taaattgcga gcgagatcgc gtcttcgatt gactgcaatt taaccaatta aattctaaaa
    27781 taatcacgaa aaaaatttta cttccgcctc atgcggcgaa tgtgggaatt gcccaggcgg
    27841 cgggggatag gggctggaga cagttatcca ctattcctgt ggataaccat gtgtattaga
    27901 gttagaaaac acgaggcaag cgagagaata cgcggcttgc acgcgaattg gcgttaaaga
    27961 cggctcaaag aaatatcttt tattttttaa ctggttagat aaatgcaatg gcagtcactg
    28021 aacaggcatc tcttgccata aaactgtcat cactcatctt gacaaatgtt aaaaaagccg
    28081 ttgctttggg gataacccgg taaggccgga gttttatctc gccacagagt aaattttgct
    28141 catgattgac agcggagttt acgctgtaac agaaatatta tggtgatgaa ctgttttttt
    28201 atccagtata atttgttggc ataattaagt acgacgagta aaattacata cctgcccgcc
    28261 caactccttc aggtagcgac tcatgagtaa accgttcaaa ctgaattccg cttttaaacc
    28321 ttctggcgat cagccagagg cgattcgacg tctcgaagag gggctggaag atggcctggc
    28381 gcaccagacg ttacttggcg tgactggctc cgggaaaaca ttcaccattg ccaatgtcat
    28441 tgctgacctt cagcgcccaa ccatggtact tgcgcccaac aaaacgctgg cggcccagct
    28501 gtatggcgaa atgaaagagt tcttcccgga aaacgcggtg gaatatttcg tttcctacta
    28561 cgactactat cagccggaag cctatgtacc gagttccgac actttcattg agaaagatgc
    28621 ctcggttaac gaacatattg agcagatgcg tttgtccgcc accaaagcga tgctggagcg
    28681 gcgtgatgtg gttgtggtgg cgtctgtttc cgcgatttat ggtctgggcg atcctgattt
    28741 atatctcaag atgatgctcc atctcacggt cggtatgatt atcgatcagc gcgcgattct
    28801 gcgccgactg gcggagctgc aatacgctcg taatgatcaa gcattccagc gtggtacttt
    28861 ccgcgttcgt ggcgaggtga tagatatctt cccggcagaa tcggatgaca ttgcacttcg
    28921 cgtggaactg tttgacgagg aagtggaacg attgtcgtta tttgacccgc tgaccgggca
    28981 gattgtttcc actattccac gttttaccat ctacccgaaa acgcactacg tcacaccgcg
    29041 cgagcgcatc gtacaggcga tggaggagat caaagaagag ctggccgcca gacgcaaagt
    29101 gctgttggaa aacaacaaac tgctggaaga gcagcggctg acccagcgta cccagtttga
    29161 tctggagatg atgaacgagc tgggctactg ttcggggatt gaaaactact cgcgcttcct
    29221 ctccggtcgt ggaccgggtg agccaccgcc gacgctgttt gattacctcc ctgctgatgg
    29281 gctgctggtg gtcgatgaat ctcacgtcac cattccacaa attggcggca tgtatcgcgg
    29341 tgaccgggcg cgtaaagaga cactggtgga gtacggcttc cgcctgccat cagcgctgga
    29401 taaccgtccg cttaagtttg aagagttcga agcattagcg ccgcaaacca tctatgtttc
    29461 ggcgacgccg ggtaattacg agctggaaaa atccggcggc gatgtggtgg atcaggtggt
    29521 gcgtccaacc ggattgcttg acccgattat cgaagtgcgg ccggtggcga cacaggttga
    29581 tgatcttctt tcggagattc gtcagcgagc ggcaattaac gaacgcgtac tggtcaccac
    29641 actgaccaag cgaatggcgg aagatcttac cgaatatctc gaagaacacg gtgagcgcgt
    29701 gcgttatctt cactcagata tcgacaccgt cgagcgtatg gagattatcc gcgacttgcg
    29761 tctgggtgag ttcgacgtat tggtagggat caacttactg cgtgaaggtc tggatatgcc
    29821 ggaagtttcg ctggtggcga tcctcgacgc tgacaaagaa ggcttcctgc gttccgaacg
    29881 ttcgttgatc cagaccattg gtcgtgcggc acgtaacgtt aacggtaaag cgattctcta
    29941 cggcgataag atcaccccat caatggcgaa agcgattggc gaaaccgaac gtcgccgcga
    30001 gaaacagcag aagtacaacg aggaacacgg cattacgccg caaggcttga acaagaaagt
    30061 ggtcgatatc ctggcgctgg ggcagaacat tgccaaaacc aaagcgaagg gcagaggaaa
    30121 atcgcgcccg attgttgagc cggataatgt gccgatggat atgtcgccga aagcgttgca
    30181 gcagaaaatc catgaactgg aagggctgat gatgcaacac gcgcagaatc tggagttcga
    30241 agaagcggca caaattcgtg accagttgca tcagttgcgt gagttgttta tcgcggcatc
    30301 gtaacaggat agcgaagaag actgatgaca aacggaaaac agcctgatgt gctacgctta
    30361 tcaggcctac attttctccg caatatattg aatttgcgcg gtttgtaggc cggataaggc
    30421 gttcacgccg catccggcat aaacaacgcg cactttgtct tcaataggaa gccggaattt
    30481 tccttcggat ttccgttaac ctaaagcctg taacgccttt tccagcgcgt tatgtaacaa
    30541 ctggcggtca tgacgatacg gaatatcgct ggcctccagt acctcctgga tcacaatccg
    30601 ctctttcacc gccgagacat ccacttttgg cccgacgatg accgcatcaa tgaccttttt
    30661 accaacatac tgctccataa ttgccagctt gctttccagc ttcaaattag ccgcaggtaa
    30721 actcaactca cgccccagat tgccgatata aaccatcggc gctggcgtgc ggcgtaatgc
    30781 ctgggcgatt tccttcagca gcagaattgg catcaggctg gtataaaaac tgccaggccc
    30841 aataatgatg agatccgctt cattgatagc gtgaaccgcc tcacgcgttg cgggtacatt
    30901 aggcgttaac aataactctt gaatcggcgt agttaactgg tcgatattga cctcgccgta
    30961 aacttcatgc ccctgatcgt caatcgccat cagatcaaca ggatgctctg acattggaat
    31021 caaatgcgta tccactttca gcagattacg aattaaattg atggcttcca gaggccgcac
    31081 gctaaggtga tccagcgcct ttaacatcaa gtttccgaga ttatgaccgg aaagttcgcc
    31141 attgccacca aaacggtatt caaacatcgc ggaggcgacg ctcggttccg ttatcagctg
    31201 gttgaggcag ttgcgcatat cgccccaggc aatgccgcct tctgaacggc gaatacgccc
    31261 cgtcgagcca ccattatcgg tggtggtgac gatacccgtt aaacgagaac ccaaagacga
    31321 aagtgatgag agaacgcgtc ccagtccatg ccctccgccg agagcaacga cacgatcaag
    31381 atcagccagc gtacgattgc gcatatagtt tcctggagtc agattatccg cgctacagta
    31441 gcgcaaattg cggggaaaca gcaattaacc tgccaatatt gaggatacaa actattttct
    31501 tctacctcta aaggacgatg cacgctatgc ctccctgatg atgtatatca aagtaaaacc
    31561 gccattttcc cttattctgt agcgaaatag cacgatcatg acgctatata catgattaca
    31621 tagcgaaagt gtggatggta aaaatctcat ttacacgcta gtatcggcat aaccactaaa
    31681 cactctagcc tctgcacctg ggtcaactga tacggtgctt tggccgtgac aatgctcgta
    31741 aagattgcca ccagggcgaa ggaagaaatg acttcgcctc ccgtatctgg aaaggtgtac
    31801 atggcttcac aactgactga tgcatttgcg cgtaagtttt actacttgcg cctgtcgatt
    31861 accgatgtgt gtaactttcg ttgcacctac tgcctgccgg atggctacaa accgagcggc
    31921 gtcaccaata aaggctttct taccgtcgat gaaattcgcc gggttacgcg cgccttcgcc
    31981 agtctgggca ccgaaaaagt gcgcctgaca ggcggtgagc cgtctttacg ccgcgacttt
    32041 accgatatca tcgccgctgt gcgggaaaac gacgctatcc gccagattgc ggtcaccacc
    32101 aatggttacc gtctggaacg cgatgtggcg aactggcgcg atgcgggact tactggcatt
    32161 aacgtcagtg tcgacagtct ggacgcccgc cagtttcacg ctattaccgg gcaggataaa
    32221 ttcaaccagg tcatggcggg gattgatgct gcatttgagg ccggttttga gaaggtcaaa
    32281 gtcaataccg tgctgatgcg tgatgttaat catcaccagc tcgacacctt tctgaactgg
    32341 atccagcatc gccctatcca gctgcgtttc atcgaactga tggaaacggg cgagggcagc
    32401 gagctcttcc gtaagcatca catctctggt caggttctgc gtgacgagct actgcgtcgc
    32461 ggctggatcc accaattacg tcaacgcagc gacggtcccg cgcaagtctt ttgccatcca
    32521 gattacgccg gagagattgg ccttatcatg ccgtatgaaa aagacttctg cgccacttgc
    32581 aaccgcctgc gcgtttcctc cattggtaaa ctccatctct gcctgtttgg tgaaggcggc
    32641 gttaacctgc gcgatctgct ggaagacgat acccagcaac aggcgctgga agcgcgtatt
    32701 tcagcggcgc tgcgggagaa gaaacagacc catttcctgc atcaaaacaa caccggtatt
    32761 acgcaaaact tatcgtacat tggcggctaa aacgtcaaaa ggagagatca gatgagtcag
    32821 gtaagcactg aatttatccc gacccgtatt gctattctta cggtttctaa tcgtcgcggt
    32881 gaagaagacg atacctccgg tcactatctg cgcgattcgg cgcaagaagc gggccatcac
    32941 gttgtcgata aagccattgt gaaagaaaac cgctacgcta ttcgcgctca ggtatctgcg
    33001 tggatcgcca gcgacgatgt acaagtggta ttgattacgg gtggtactgg cctgacggaa
    33061 ggtgatcagg ctcccgaagc attgctgccg ttgttcgacc gtgaagttga aggttttggt
    33121 gaagtgttcc ggatgttgtc ttttgaagag attggcactt ccacgttgca atctcgtgcg
    33181 gtagcgggcg tcgccaacaa aacgctgatt ttcgccatgc cgggttcgac caaagcgtgc
    33241 cgtaccgcat gggaaaatat catcgcgccg cagctggatg cccgtacgcg tccgtgtaat
    33301 ttccatccac atttgaagaa ataagtatgt cgcaactgac ccatatcaac gccgctggcg
    33361 aagcgcacat ggtggatgtc tccgccaaag cggaaaccgt gcgtgaagcg cgggcggaag
    33421 cctttgtcac catgcgcagc gagacgctgg cgatgattat tgatggtcgc caccacaaag
    33481 gcgacgtatt tgccactgcg cgtattgccg gtattcaggc ggcaaaacgc acctgggatc
    33541 tgatcccgct ctgtcatccg ctgatgctca gcaaagttga agtcaattta caggccgagc
    33601 cggagcacaa tcgggtgcgt atagaaacct tatgccgcct gaccgggaaa accggtgtcg
    33661 aaatggaagc attaaccgcg gcctccgtgg cggcgctgac catttatgac atgtgcaaag
    33721 cggtgcaaaa agatatggtg attggtccgg tacgtttgct ggcgaagagc ggcggcaagt
    33781 cgggtgactt taaggtggaa gcggatgatt aaagttcttt ttttcgccca ggtgcgcgag
    33841 ttggtgggaa cagatgcaac cgaagtggct gcggatttcc caactgttga agcgttacgc
    33901 cagcacatgt ctgcgcagag cgatcgctgg gcgctggcgc tggaagatgg caaattactg
    33961 gctgccgtca accagacgct ggtgagtttt gaccatccgc tgactgacgg cgacgaagta
    34021 gctttcttcc cgccggtaac cggaggttaa gatggcagaa accaaaattg ttgttggtcc
    34081 gcagccgttc agcgtaggag aagagtaccc gtggctggcg gagcgtgacg aagacggtgc
    34141 ggtagtcacc tttactggta aggtgcgcaa ccataacctg ggcgacagcg tcaaagcatt
    34201 aaccctcgaa cactatccgg ggatgactga aaaagcactg gcagaaattg ttgatgaagc
    34261 gcgtaaccgc tggccgctgg ggcgcgtcac tgtgattcac cgcatcgggg aattatggcc
    34321 gggcgatgaa atcgtttttg tcggtgtcac cagtgcgcat cgcagcagtg cgtttgaagc
    34381 cgggcagttt attatggatt atctcaaaac ccgcgcaccg ttctggaagc gcgaagccac
    34441 gccggaaggc gaccgctggg ttgaagcccg ggagagcgat cagcaggcgg caaaacgctg
    34501 gtagtttttt gttagccgga taaggcaccg ctgcgtccgg caatttcacc gctcagtaca
    34561 tacgtttacg ctgcgtcgat gcacagcctc atcattttgc agtatcctta agatattcct
    34621 tatatcttca ggagatcgtc atggacagat tcccacgttc tgattcaatc gtacaacccc
    34681 gggctggctt gcaaacttat atggcgcaag tctatggctg gatgaccgtt ggcttgttgc
    34741 tgaccgcatt tgttgcctgg tatgcggcta attccgcggc cgtgatggag ctgttgttca
    34801 ctaaccgtgt ctttttaatc ggtctgatca tcgcgcaatt agcattggtt attgtgttat
    34861 cagcgatgat tcaaaagctg agcgcaggtg taacgacgat gctctttatg ctttattcgg
    34921 cgctgacggg tcttacgctt tccagtatat tcattgtcta taccgctgct tctatcgcca
    34981 gtactttcgt cgttactgcc gggatgttcg gcgcaatgag cctgtacggt tacaccacga
    35041 agcgcgattt aagtggcttt ggcaatatgc tgtttatggc gttaatcggc attgtgctgg
    35101 catcgctggt caacttctgg ttgaaaagcg aagcattgat gtgggcagtt acctacatcg
    35161 gcgtgattgt ctttgtcgga ttgacggcgt atgacacgca gaaactgaaa aatatgggtg
    35221 agcagattga tacccgcgac acgtcgaacc tgcgcaaata ttccattctt ggcgcgttaa
    35281 ccttgtatct ggacttcatc aacctgttcc tgatgttgtt gcggatcttc ggcaaccgcc
    35341 gttaaattct cttcttcctg ctgatcccgg cctggagccg ggattttttt cggattttat
    35401 aaagactatt ccgctcattt acatgagaac tgaatcgcgg caaatgccag actatgtcca
    35461 ctacgtaatt tcagggacag ataacgcatt tgaacagaca atccactgct actcaattac
    35521 agcaaaaaag tgcttcgctc agcaaaatgc tgcttctcat ttggtggtta acagcagctg
    35581 gagtatgggc tacggcaggc tgtgcctgga tatttcagca tttgcattgg gttacgtcac
    35641 aaaagtctcc aggtgcgttt gtcttcgctt tggctgcgct gataataggt ggtctctggt
    35701 taaacaacgc cctgaaaaga ctgaatgtat caggcgcatg tctggtctat ctgctaataa
    35761 tccttcttgt tggctttctc ttcgccagcc tttatacctt ttcgagcata gcgacggtaa
    35821 tgggaattac gggagggatg tttgccgcaa tggcgctgat ttgctcttgt agtaataggg
    35881 ttatcccacc agtcagacag ttatattcat atattttctg tggtttgagt attgcttttg
    35941 ttgttaacct gatccttacc agcagttttt ccgtgtggtt agcgagtatt ttgactgtct
    36001 ttatctgggg cattacggct gcctgtgaag caacaacact ggaagatttg atccgtgttg
    36061 ccgacacctc tgaaatttca ggttctcttc gctgtatcgt tcctggggca ataacactct
    36121 attttagtat tttaagcgtc ctctttagaa ttactgtaac agttcttgaa tttattaatg
    36181 gtttagtgtg gtagtgcgaa ttgacgagtt ttattgcgtt aattggaata gataatgata
    36241 tttaacttaa ggatataaaa ataatgaatt cgcgctcagc aaataacata actaatgatt
    36301 ttcagcacga agccaggata ttaaacagca tatggctcat taccgcttta ggtttggtga
    36361 caaccgcagg actggcctgg gtagcgaagt atctcgaaat tacggcaaca aaatatgatt
    36421 caccgcaaat gtatgtcgcc ataggattat tattactttg tatgtatggc ttaagtaagg
    36481 atatcaacaa gataaatgcc gtcatcgcag gcgtaatata tctattttta ctctctttgg
    36541 tggcgattgt cgttgctagt ttagctcctg tacctgccat tatcgtcgtg ttcagcactg
    36601 cgggcgcgat gtttttaatc agtatgctgg ccggtttatt atttaatgtt gatcctggtt
    36661 ctcaccgttt tatcattatg atgacgttga cagggttggc cctggtgatc atcatgaatg
    36721 ccgcattaat gagtgaacga cccgtttggg taataagttg cttaatgatt gtgttatggt
    36781 caggcattat ctcgcatgga cgaaataagc tccttgaatt ggcggggaaa tgccatagtg
    36841 aagagttgtg gagtccggtt cgttgcgctg ttacaggtgc attaacactc tattactatt
    36901 ttatcggctt ctttgggata cttgccgcga tagctataac gcttgtctgg caaaggcata
    36961 cgcgtttttt tcattagtcg taaatgcaga tactaaagaa cttaactaaa cttcacatcg
    37021 ccgcttcatt tttcgcccgt agcttcttcg cctggctttc cagtagcagg taacagatca
    37081 gcgccagcag cagcgggata aagtaataca ggacgcgata agcgagcagg gcggcgataa
    37141 tgctcccttt cgaggtatgc tccccagcca gtagtgcgat aaacaccgct tccagcacac
    37201 cgatccccgc cggaatatgc acgatgacgc cagcaatact actaaccagt aacacgccca
    37261 gtacaaagaa atagttcacg ctttgaccaa gtaacagcca gataatcgcc cccattacca
    37321 tccagttaac gctggaaatc agcatctgag cgagggcgaa tttccatgaa ggcagcacca
    37381 gcttttgccc tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa
    37441 ccgcgataat catcagtaag ccaatgccga gaatacgcag cgtagtttga tcgacatacc
    37501 agtgatccgg caactccacc acgcctgcgg taaagataat ccctgccagt aaaatatagc
    37561 ccagccagtt ggtggtaata ctgagcgaga aaatccgcgt aatagtgctg cccggtaacc
    37621 ctagtcggga gtacaaacga tagcgcatac caatgccgcc gacccaggta ctgagcgtca
    37681 ggttgaaggc gtagcagata aacgacacca gcatcacctg gcgcttcgcc agtttgtgac
    37741 cgcagtaaaa gcgggcgagc aggtcatagc agccgtaaat cagatagctg acgaccacca
    37801 gcccgaccgc actaagcagc gcaacgcgat tgtagtcgcg gatgaccttc cagacctctt
    37861 cccagtccac ttttttggcg tagaccacca gcaacacaat caccgcgata aaaaacagcc
    37921 aggtgaggat cttctttgtt aagcgccagc gcgggtgtga tttactcatc agggttttac
    37981 ccccgtgttt tcagtttcta cccgatcctg cgtttccatt gtcggttgtg cgggcggatc
    38041 aacctgcgcc agacgtggtg tgtgtgccgg aagccagcca accagcgccg ggaagtggcg
    38101 taaaaagtgg aacgccagca cgcttttggt caggttccac caggtgcgtt tcggcagcat
    38161 ggtctcatcg acctgctgac aatctgcggc aataatgccg ttcagattat cgcgcagcgt
    38221 ctggttaaaa tgacgatcgt ggatgatgac atttgcttcg agattcagtg acaaactgag
    38281 cggatcgaga ttactggacc ctactgtcgc ccagtgatcg tccatcaatg ccactttgcc
    38341 gtggagcggg cggcggcggt actcaaatac ctgaacgccg cctttaacca gatagttata
    38401 cagcaagcgc gcaccgactc tgacaatcgg catatccggt tcgccctgaa tgatcagttt
    38461 gatccgcacc ccgcgccgtg ccgctttacg caaggcgtgt aaaaatcgat agccggggaa
    38521 gaagtaggcg ttggcgataa tgacttctcg ccgcgcctga gtgagcattt tcagatagtg
    38581 acgttcaata tcatcgcgat gttcttcgtt atcgcgccag accagcaata cctgcgcttc
    38641 tcccggctgg cggttctctt ccgctttgtg atgacgtcgc caccagcgtc gtgccgcgct
    38701 ctgtccaggc aggttttcca gctcaaactg gagaatatct tcgacaatcg gcccttcaag
    38761 gcgtaccgcg taatcctgtt tagcctctgg accgtagctg gacatatgct cggcggagta
    38821 attcagcccg ccaataaagg cgatacgcgc gtcgatcacc acaattttgc gatgcatccg
    38881 gcgaaacaca ttggtgcgca taccaaaaag gcgagggcgg ggatcgtagt agcgaaatac
    38941 cacgccagct gccgtcagtt cattgacaaa ctcatcgctg agatccggcg aaccgtagcc
    39001 atccagcaag acttccgctt taaccccgcg ttgcgctgct gccagtagtg ccgcatgcag
    39061 ctgtttgccg acgtcatcct caaaccagat aaacgtttca agaatgatgc gttcttgtgc
    39121 ctcgccaatc gccttaaaca ccgcgggata atattgctcg ccgttttcca gcaactggat
    39181 cttattgcct tcgcgccagc tacatttcat aaatgaatct ccgcacttaa aggggcatga
    39241 tcagaaaggt gtcgccatgt ccgcagcggc aacgcggttg gcgcgctggc gctggcattt
    39301 ttgacgtaga tcctgtccag tcgtagtaga ggaaattgca ccggaaacgt gcgcgccggg
    39361 cgtccgtggg cgcgggtaaa aatctcatcc agtccggcct gcacttttaa cggatgatta
    39421 gctttttgcc gccagtcatt gaaatcaccc gccaccaata ccggttcgcc gtccggtagc
    39481 tcattcaccc attcggcgag catcgcaagc tgcgcctgac ggtgcgcctc acgcaggccc
    39541 agatgtacgc acatcacatg aatcgctttt ccggtcatcg gtggcacaat gcggcagtag
    39601 agcacgccgc gcttttccgc accatcgacc gaaacatcgc gattctcata atgttcaatg
    39661 ggataacgcg acagtacggc gttgccgtga tgcccttccg ggtatacggc attgcgaccg
    39721 taggcaaaat cgctccacat cgtgtcggcg agaaactcgt agtgcgaggt atcgggccag
    39781 ttttccacat gcagcggatg aacttcgtgc gcgcccatca cttcctgcag gcaaacaata
    39841 tcggcgctga cggtacgcac cgcgtcgcga agttccggca aaatgaagcg tcggttaaac
    39901 gcggtaaaac ctttgtgaat gttgatggtg agcaccttga acgaaaattg ttgtgtttga
    39961 tcgggcataa ttttcctgtc tttgcctctt atctcattga aatagtgtag tcggcgtcac
    40021 aaaaaggtgc ggtcttacgg aattttccgt aaagttcggt actctgagta agtagagata
    40081 aattcttcag gagagaagcc atgaagtggc aacaacgtgt tcgtgtcgca actggtctaa
    40141 gttgctggca gattatgttg catttactgg tagtggcgct gctggtggtg ggctggatga
    40201 gtaagactct ggttcacgtc ggcgtgggat tatgcgcact gtattgtgtc acggtagtga
    40261 tgatgttggt gtttcagcgc caccccgagc aacgctggcg tgaggtggca gacgtgctgg
    40321 aagagctgac cacgacctgg tattttggcg cagcgctgat tgtgctgtgg ctgttgtccc
    40381 gtgtactgga aaacaacttt ttgctggcaa ttgcagggct ggcaatcctt gccggcccgg
    40441 cggtagtgtc tttgctggcg aaagataaga agttacatca ccttacgtct aaacatcgcg
    40501 tacgccgctg accctgtcgt ggccgttatc accagtagcg gccacaaact attccacaca
    40561 atatccagac tcgcatcctt caaataaatc tgcttggtaa tgtccgtaaa gtggcgaata
    40621 gggttaatcc acgtcaggtt ttgcagccat actggcatgt tttccaccgg agaaacgtaa
    40681 ccggaaagga gaatggcggg catcataaaa acaaacacgc cgataaacgc ctgctgttgt
    40741 gtcgagcaga gtgatgaaat caacagaccg aatcccacca gcgataaacc gtaaatcacc
    40801 atcgtaaagt agaacagcgc cagcgatccg gcgaagggga tttgatacgc ccagatacca
    40861 atcgccagca caatggtggc ctggaaggtg gcgacaatta acgccggtac ggctttgccg
    40921 atgaagatct gccaggtggt gagcggcgaa accagtagct gatcgagcgt accttgttca
    40981 cgttcgcggg cgacggaaag tgaagtgacg atcattacgc cgatagtggt gatcatggcg
    41041 atcagcgacg gcaccacaaa ccatttgtag tcaagattcg ggttatacca gttgcgtacc
    41101 accagctcgc tgttgttagg tttcggtttt ccttccagca gctcctgctg ataatttttg
    41161 acgatctgtt gcaggtagtt ggcggcaatt tgcgcactgt tggagttacg cccgtcgagg
    41221 atcaactgca acggcgcggt ctggaaggta tccagtttgc gcgagaagtc agccgggaaa
    41281 cgcaccagta gtaacgcctt ttgtgtgtcg atggttgggc ggatctcctg tgggcttttc
    41341 agcagcagca catgagtaaa ggcgctggcg cgggcaaaac gttgggtcag ctccaccgaa
    41401 tgctcgccgt tatcttcatc gtagatggcg atggtggcgt tagtcacttc cagcgtggcg
    41461 gcgaacggga acagaatcac ctgaataagc acaggtaaaa tcagaatcgc gcgggtttgc
    41521 ggttcgcgca gcagcgactg caactctttg cggattaacg tccataagcg atgaaacatg
    41581 ctcttctccc taatccagcc gacgtttggt tttcagccac gtcaggccga taaacatcac
    41641 cgccgaagcg atcaaaaaca gcacgtttac caccagcacc actggaatat tcccggcgag
    41701 gaacaggctt tgcagggtgc tgacgaaata acgagcggga ataatgtacg tcaccgcgcg
    41761 gatcaccgcg ggcatactgt cgatctgaaa aataaagccg gaaagcataa tcgacggcag
    41821 aaaagcggcg ttcagggcga cctgagcggc attgaactgg ttgcgggtaa tcgtggaaat
    41881 cagcagcccc atccccaggg tactgagtaa aaacaggctg gagataaaaa acagaatcag
    41941 cagcgacccg cgatacggca cgccgagaat aaacactgac accagcatac acagcaacat
    42001 cgccagcatc ccgagaaagt aataagggat cagcttacac agcagcagtt ccgtgcgggt
    42061 aatctccgta gagagcagag cctccatggt gccgcgttcc cattctcgcg ccaccaccag
    42121 cgaggtgaga atcgcgccga tgaccgtcat gataatggtc accgcaccgg ggataatgaa
    42181 gtgctggcta atcgccgccg ggttaaacca gtagcgggtt tgtacatcaa taagcggttc
    42241 aaaagtctgc ccgttgtcct ccgctcgctg catttgccag atctgccaga tcccttcgac
    42301 atacccctgt acaaagttag cggtattcgg ctcactgccg tcggtgatca cctgaatcgg
    42361 tgcggtggcg ttggcgcgct ccatctgttc cgcaaaatcc accggaataa ccaccagacc
    42421 gcgaattttc cccgcctgca ttttggcgat cagttcctga cggttatcgc tgatggtggc
    42481 gtcgatgtag ggcgaaccgg tcatggtgtg ggtgaaatcc agcgccgctt cgctacgctg
    42541 ttccagtaaa atcccgaccc gcagcttgct ggagtcgagg ttaatgccgt aaccaaaaat
    42601 aaacagcagc aacagcggga tcactaccgc aatcagccag ctactcggat cgcgaacgat
    42661 ctgccgcgtc tctttgacgc acagcgcccg cacgcgacgc caggaaagga tcgggttact
    42721 cattgctatg ctccttatcc cagtcgtgga tcaactgaat aaaggcttgc tccatggtgg
    42781 gatcgggttg ctcgtcgttg gccgactgcg ctttcaaatc atccggcgtg ccgctggcaa
    42841 ttaatttccc acggtacacc aggccgatgc ggtcgcaata ttctgcttca tccataaagt
    42901 gggtggtgac catcaccgtg acgcctttct ctaccatgct gttgatatgt agccagaatt
    42961 cgcggcgagt aatcggatca acgccggacg tcggttcgtc gagaaacaga atgtccggtt
    43021 catgcatcag cgagcaggcc agtgccagcc gctgtttaaa acctaatggc agttcatcgg
    43081 tggcgtggga ggcgatactt ttcaggccga acgcctcgct catacgggag attttttcgt
    43141 tctgcgcccg accgcgtaag ccatacacac cagagaaaaa gcgtaaattc tgttcgaccg
    43201 tcaggttacc gtagagcgaa aacttttgcg ccatatagcc gagatgctgg cgcgctttac
    43261 cagaactctc tttcagatcc atccccagca ccagcgcctg gccggaagtc ggcaccagca
    43321 aaccgcacat catcttaaag gtggtcgatt tacccgcgcc gtttggcccc agcaaaccaa
    43381 aaatctcccc acgtttaacg gcaaagttga cgtgatcggt ggcggcaaaa tcaccaaatt
    43441 tcttggtcag ttctttcgct tcgatcaccg tctcgccggg tgtgccttct accgtatgta
    43501 atattgcgcc cagcggcgat tctgaggttc cggcaccacc cagcaaatca ataaatgcat
    43561 cttcaaaacg cggcgtagtt tcgttgatgt tgatttccgg catcccgtcg gcatggcgaa
    43621 tatcgtctgg tgtggcctct tttttgagga tcagacgtac cgatttcccc tgaatcatgc
    43681 cgtcgctgac ctgtggcagt ttcaaggcgc gttgcaatag tttgcggttg ccctcgtgtg
    43741 gactggtcat cagaaaactg cgtccggcca tggtttgtgt cagggctttt ggttccccct
    43801 gatacagcaa ctcgccttcg ttcatcagta acacgtcacg gcactgctcg gcttcgtcga
    43861 gatacgaggt actccagagg attaacatcc cttcgcctgc cagctcatgc accatctgcc
    43921 acagttcgcg ccgtgagata gggtcaacgc cgacgccggg ttcatcgagc agcaacactt
    43981 tcggttcgcc caccagggta caggccagac cgagtttttg tttcatccca ccggagagct
    44041 tgcccgccag gcgtccggta aacggcccaa gagacgtaaa ctccagcagg cgagcaaaag
    44101 tttgcttacg tgcctcgccg gtgacgctgc gcaaatccgc gtacagattg aggttctcca
    44161 tcaccgtgag atcttcatac agaccaaatt tctgcggcat atagccgagc acggcgtgca
    44221 gcgcgccgtc gtttttgatt ggatcaaagc caatcaccgt ggcattgccg ctgtcgggtt
    44281 tcagtaatcc cgccagcatc cgcattagcg tggttttacc tgcaccatcc ggccccacca
    44341 gccccgtcac ataaccggcg tgaatggtac aatcgagcgg cgcgacggcg ggcttgtcca
    44401 tgcccggaaa gcgtttttcc aggccgttca gcgtgataac ggcatcattc atgtcctgcc
    44461 tcgttaccga attgcaccgt caccggcatt ccctggcgta acgcatcatc ggcgtcggtc
    44521 accacaatac gcaggcgata gacgaggtcg gtacgcagat ccggtgtttc gacggttttc
    44581 ggggtaaatt cagcagtcgg cgaaacgaaa ccaatctgcc cgtgataggg cttgtccggg
    44641 cgaccatcgg tataaagcag cactttgcgc cccggctggg cctggtcaag attccgttca
    44701 tcaacatagg cacgtaccca caccggacgc gttagtgaaa cggtaaacac cgtgccacct
    44761 tcattgagaa ccgtgcctgg ctccaccgcg cgcgttaaca gcgtgccatc agacggggct
    44821 atcaacgttg agtcctgcaa attcaactcc gcctgcgcca gttgcgcctg cgcctgttcg
    44881 aggctggctt tcgcctgggc gatgtcctgt tcacggttac cggaacggta ctgacgcaat
    44941 ttatcctgtg ctgatttcag cgttgcctgc gcctggtcgc gcgaggagcg ggcattttcc
    45001 aggtcgtttg ccgaaatggt gcggcttttc cacagtcctt gctggcggtt atagaagttc
    45061 tgcgcatagt cataggcggc ttgcgcctgt ttcaccgctg cggcggcctg agcgatttct
    45121 tcatcgcgat acccggcaag catcaggtca tactgcgcct gtgccaccga gacgcccgct
    45181 ttcgcctgca tcagggcaat ctcatacggc ttgtgatcca gttcgcccag tacctggccc
    45241 gctttgatcg catcaccttc gtccaccgcc agcgactcaa cgcgaccacc gacacggaag
    45301 ctcagattta cggtgcgaat atccacgttg ccatacagcg tcaggccgtt atcctggcgg
    45361 ctttgatacc accagtagcc tccggcaacc acggcggcaa gtaccactac cgccaatccg
    45421 atcacgacag gttttttcat cactacaaac tcctttgcga taatccttgc aaaatcaggt
    45481 cgatatgaca ggtcaccgtc tggttgatca gttcggtctt ttcttcatcg aacgcggtcc
    45541 agccggtacg taacagaatt gtttctttgc caagacggaa cgccagaatc tcaccaatca
    45601 acgcatgagt atggaggatc atgcgggtat cgttggcgtc gcagccggtc caggcggcaa
    45661 tcaggcgtgt caggtggctg tgtagcgggc taatcacctg ttcgtgcacc aggtgatagg
    45721 ctgccgtggg agagagctgc tcacgggaga taaacttgct gaggttgacg gtgtcatcct
    45781 gggtgagcag cttaatcatg ttcctgcagg cgcgaaggat cagttcacgg atggctgcgc
    45841 gatcaggctg tggttgtgcg aacaagcgtt cggcctcttc ggcgtgcgga cggaactgct
    45901 cgccaataaa atcggcaatc cactgggcgc aggcgaggta caaatcttct ttcgaaccga
    45961 agtagtaggt gatggcagcg atattctgcc cggcctgggc ggctatctcg cgggtggtgg
    46021 cgttcattcc atattcacca aactgcgcca gtgcggcagc aatcagctgt tttttcgcct
    46081 gttcaccctt gattgtcatg gcaggattat tcatcgcaca gtccattctt aatcaaatga
    46141 ttgattaaga ttatgactcc ataggggagt tgtccagtat ggctaagaat tttagctacg
    46201 ccagtcacag ggataattta tgcgctgcgt cacaaaaact gctacactcc gctccctcat
    46261 gacattgtgg tttttgtcat tttccttttc agtatctccc tgaaaactac accggtaacg
    46321 gtcggggcgg ttcggagtag ttatgtcttt cgattctttg ggtttaagcc ctgatatcct
    46381 gcgcgccgtt gccgagcagg gttaccgtga acccacccct attcagcagc aggcgatccc
    46441 tgcggtgctg gaaggccgcg acctgatggc tagcgcccag accggtaccg gtaaaacagc
    46501 gggctttacg ctgccgctgt tgcaacacct gatcactcgc cagccgcacg ccaaagggcg
    46561 tcgtccggta cgtgcgctca ttcttacccc gacccgtgaa ctggctgcgc agattggcga
    46621 aaacgtccgt gattacagca aatatttgaa cattcgttcg ctggtggtgt ttggcggtgt
    46681 cagcattaac ccgcaaatga tgaagctgcg cggcggcgtt gatgttctgg tggcaacacc
    46741 gggacgtttg ctggacctgg aacatcaaaa tgcagtgaag ctggatcagg ttgaaatcct
    46801 tgtcctcgat gaagctgacc gcatgctcga catgggcttt atccacgaca tccgtcgcgt
    46861 gttaacaaaa ctgccagcga aacgccagaa cctgttgttc tccgcgacct tctctgacga
    46921 tattaaagcc ctggcggaaa aactgttgca caacccgctg gaaatcgaag tggcacgccg
    46981 caataccgcg tctgatcagg tgacccagca cgttcacttt gtcgataaga aacgcaaacg
    47041 cgaattgttg tcgcacatga ttgggaaagg gaactggcag caggtgctgg tgtttacccg
    47101 taccaaacat ggcgctaacc atctcgctga acagcttaat aaagatggca tccgtagtgc
    47161 ggcgatccac ggtaataaat cgcaaggtgc gcgtacccgt gcgctggccg attttaaatc
    47221 gggcgatatt cgtgtactgg tggcgaccga catcgctgcg cgcggtctgg atatcgaaga
    47281 gttgccgcac gtcgtcaact atgaactgcc aaacgtaccg gaagattatg tccaccgtat
    47341 cgggcgtacc ggtcgtgcgg ctgctaccgg tgaagcgttg tcgctggtgt gtgttgatga
    47401 acacaaactg ctgcgtgata tcgaaaaact gctgaaaaaa gagatcccgc gcattgcgat
    47461 tccgggctat gagccggacc cgtcaatcaa agccgaaccg atccagaacg gtcgccagca
    47521 acgtggcggc ggcggtcgtg ggcaaggtgg tggtcgcggt caacagcaac cacgccgtgg
    47581 ggaaggtggc gcaaaatctg caagcgcgaa acctgcagaa aaaccgtctc gccgcctcgg
    47641 cgatgccaaa ccggcaggcg aacaacaacg tcgccgccgt ccgcgtaaac ctgctgctgc
    47701 gcagtaatct ttaatgccgg gctatgcccg gcatcaggct gatgaacaaa cgcaaaactg
    47761 cctgatgcgc tacgcttatc aggcctacgt gaactctgca atatattgaa tttgcatgct
    47821 tttgtaggcc ggataaggcg tttacgccgc atccggcatt tcacaacaag cacttgtcag
    47881 caatttgaga acacgggaaa aaatttatct gttttaccgc ccataagcca ccgagacgtt
    47941 acttctctat agccaattgt tcgcgcaact ccattaaaag gtagcccagt cgattcttgc
    48001 ccttaccatt accaccgtct ccccagtaag catcgttttc cgtatgctca accagttttg
    48061 cgggcgcggt tgccagcaag agcgcacgca gttctggatg ctgttcgaat ttagcgcgaa
    48121 gtgcttttcg catcacctgt tctttgaccg actcccagtt tttacgcaga ggcttagaac
    48181 gatcgcgccc catgcgtgcg gcgaccatag gggaagaaac ccgacggatc tcttcgcggt
    48241 atttttcatc aaggaatttt tgtgcctgaa aatagtgttc tgaggtgggc caggttttcc
    48301 cgtcaacctt gatgggccag gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc
    48361 tgtagaaatt tatgatggtg tcttgcatga cgtgttggat cctttgtgct cgaacgggca
    48421 ttaaaccgca ttatgttggt ggttattgcg agccgctttc cagaaacaga aaaaccatta
    48481 cccctgaaaa ccgaaaaatg ccacaatatt ggctgtttat acagtatttc aggttttctc
    48541 atggcattaa ccgccgcgct taaagcgcaa attgccgcct ggtataaggc gcttcaggaa
    48601 cagatccccg actttattcc ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa
    48661 acgctggccg gagaagaagg gcggcatctg gcgattgaag cccccaccgg cgttgggaaa
    48721 acgctctcct atttgatccc cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg
    48781 gtgagtaccg ccaacgtggc attgcaggat cagatttaca gcaaagattt accgctgctg
    48841 aaaaagatca ttcccgatct taaattcact gccgcttttg ggcgtgggcg ctacgtttgc
    48901 ccgcgtaatc tgacggcgct cgccagcact gagcccacgc aacaggatct gctggcgttt
    48961 cttgacgacg aactgacgcc aaataatcag gaagagcaaa aacgttgtgc gaagctgaag
    49021 ggcgatctcg acacttataa atgggatggt ctgcgcgatc atactgatat cgctatagat
    49081 gacgatctct ggcgtcgttt aagtaccgac aaagccagct gcctcaaccg caactgttac
    49141 tactatcgtg aatgcccgtt ttttgttgct cgtcgggaga ttcaggaagc ggaagtggtg
    49201 gtggcaaacc atgcgctggt gatggcggcg atggaaagcg aagccgtact acctgacccg
    49261 aaaaatctgc tgctggtgct ggacgaaggt catcacctgc cagatgtggc ccgggatgcg
    49321 ctggagatga gtgccgaaat caccgcgccc tggtatcgac tacagctgga cttgttcacg
    49381 aaactggtcg ctacctgcat ggagcagttt cgcccgaaga ccatcccacc gctggcgatc
    49441 cctgaacgtt tgaatgcgca ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac
    49501 attctcaatc tctacatgcc tgccgggcag gaggcagagc accgttttgc gatgggcgaa
    49561 ctgcctgatg aagtgctgga gatctgccag cggctggcaa aactcaccga gatgctgcgt
    49621 ggtctggcgg agttatttct taacgattta agtgagaaaa ccggcagcca cgatattgtg
    49681 cgtctgcatc ggttgatttt gcagatgaac cgcgcgttgg ggatgttcga ggcgcaaagc
    49741 aaactctggc ggctggcgtc tctggcgcaa tcttccggtg cgccggtgac caaatgggcg
    49801 acgcgggaag agcgcgaagg gcagctgcat ctctggtttc actgcgtggg gatacgtgtc
    49861 agcgatcagc tggaaaggct gctgtggcgc agtattccgc acattattgt cacctccgca
    49921 accttgcgtt cgctgaacag tttttcgcgt ttgcaggaga tgagcgggct gaaagagaaa
    49981 gcgggcgacc gttttgtggc gctggattcc ccctttaacc actgcgaaca gggcaaaatt
    50041 gttattcccc ggatgcgctt tgagccttcc atcgacaacg aagagcagca tattgccgaa
    50101 atggcggcct ttttccgtga gcaggtggag agcaaaaaac atctcggtat gttggtactg
    50161 tttgccagcg gacgggcgat gcagcgcttt ctcgactatg tgacggattt acgcctgatg
    50221 ctgctggtgc agggcgatca gccgcgttat cgcttagttg aactgcaccg caaacgcgtt
    50281 gccaacggtg agcatagtgt gctggtgggc ttacagtcat ttgccgaagg gcttgatttg
    50341 aaaggtgatc tgctcagcca ggtgcatatc cacaaaatcg cttttccgcc catcgacagc
    50401 cccgtggtga tcaccgaagg ggaatggctg aaaagcctca accgctatcc gtttgaggtg
    50461 caaagcctgc cgagcgcctc gtttaacctg attcagcagg ttgggcgact gattcgaagc
    50521 cacggttgct ggggcgaagt ggttatctac gacaaacgct tgctgaccaa aaattatggc
    50581 aagcgactac tggatgcatt accggtattt ccgatagagc aaccggaagt ccctgaaggt
    50641 atagttaaaa agaaagaaaa aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag
    50701 tcaggtaagg agtcgtaaat ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa
    50761 aaccacgcgc gcgatttaga ccgggatact gcgcgcggtc tgtatgctca tatgctcaat
    50821 ggtgaagtcc ctgaccttga gttgggcggc gtattgattg cgctgcgtat caaaggggaa
    50881 ggggaagcag agatgctcgg cttttacgaa gccatgcaaa atcacaccat caagctgacg
    50941 ccgcctgcag gcaagccgat gccgattgtc atccccagtt acaacggcgc gcgtaaacag
    51001 gccaacctga cgccgttgtt ggcgattctc ctgcacaaac tcggttttcc tgtggtggtt
    51061 cacggggtta gcgaagatcc aacccgcgtg ctgactgaaa ccatttttga attgatgggt
    51121 attacgccaa cgttgcacgg cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg
    51181 tttatgccag tcggcgcgtt ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg
    51241 atgggcgtgc gtaacagtgc acataccctg gcgaaactgg cgacgccatt tgccgaaggt
    51301 gaggcgctgc gtctttccag cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc
    51361 tttagcgata tcggcgggcg ggcgctacta atgcatggta ctgaaggtga agtgtatgct
    51421 aatccgcagc gctgcccgca gatcaatctc attgaccgtg aagggatgcg ggtgctgtat
    51481 gaaaaacagg accctgctgg tagcgagtta ctgccacaag caaaagatcc ggaaaccacg
    51541 gcgcagtgga ttgagcgttg ccttgctggc agcgaaccga ttcccgaatc gctgaaaatc
    51601 cagatggctt gctgcctggt ggctacgggt gaagcggcaa ctatcagcga cggcctggcg
    51661 cgcgttaatc aggcatttta attcttttct cagccggatg agccatgtca tccggctttt
    51721 accccaccgc aatatgaaat tcctgcatct ttattgacct tcccacgccc ggcgtgcagc
    51781 ataaaaaaac aacaaacaca taacataaac aggagttaac catggaaagt ggtcatcgct
    51841 ttgatgctca gacgctgcac agttttattc aggctgtatt tcgtcagatg ggtagcgagg
    51901 aacaagaagc gaaattagtg gccgatcatt taatcgcggc aaacctggca gggcatgatt
    51961 ctcatggtat tggcatgatc ccaagctatg tgcgttcctg gagtcagggg catctgcaaa
    52021 ttaaccatca tgccaaaatc gttaaagagg cgggggcggc agtcacgctc gatggcgatc
    52081 gcgcattcgg tcaggtcgcg gcacacgaag cgatggcgct ggggattgag aaagcgcatc
    52141 agcacggtat tgctgccgtg gcgctacata actcgcatca tatcggccgt atcggttact
    52201 gggcggagca gtgtgcagcg gcggggtttg tctctatcca ctttgttagc gtggtcggta
    52261 ttccaatggt cgcaccgttc cacggtcgcg acagccgctt tggcaccaat ccgttctgtg
    52321 tggttttccc tcgtaaagat aatttcccgc tgttgcttga ttacgccacc agcgccattg
    52381 catttggcaa aacccgcgtc gcctggcata aaggcgtccc cgtgccgcca ggttgcctga
    52441 ttgacgttaa cggcgtgccg acgaccaatc cggcggtaat gcaggagtcg ccgttgggtt
    52501 cgctgttgac ctttgccgaa cataaaggct acgcccttgc agcgatgtgt gaaattcttg
    52561 gcggggcgct ttccggcggt aaaacgacgc atcaggaaac gttacaaacc agtcccgatg
    52621 ccattcttaa ctgcatgacc actatcatca tcaacccgga actcttcggc gcgccggatt
    52681 gtaacgcgca gaccgaagcc tttgccgagt gggtgaaagc ctcgccgcat gatgatgata
    52741 agccgatttt gctaccgggc gagtgggaag tgaacacgcg tcgcgaacgg cagaagcagg
    52801 ggattccact ggatgcggga agctggcagg ccatttgtga tgcagcgcgg cagattggta
    52861 tgccggaaga gacgttgcag gctttctgtc agcagttagc cagctaaaaa aaagcccgtc
    52921 cagtggcgga cgggcaaaca agggtaacat aggatcaatg agggttagag catatgcgtc
    52981 tgtcggcaaa cagacaggga aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc
    53041 aacggttcgg attttgtagg cctgataaga cgcggtagcg tcgcatcagg catctgttgc
    53101 cggatgcggc gtaaacgcct tatccgtcct acgaattact tataaattac cgcagtaccg
    53161 cttaatttat tgttgttggt ggcggaagtg atgctgtatc cactggcacc ggctgccgcg
    53221 gctttctcag ccagtttcgc ttccagggca tcgagggtgg atgcgccatc ggcagaaacc
    53281 acgccgattt tattcatgtt ctgtgcctgg gatgccgtta ccggttccgc ggcaaacacg
    53341 ccaaatgaca gagttgaaag agccatagca gcaacaacag tattgatagt tttcataatt
    53401 aatctctcgc aggtgatttt tgtataagtg acgttgtttc gtcgatgtga tgagtatcac
    53461 gtttttttac gagagataaa atcgaagaga attgaccgcc ttgttcaaat aaattgattg
    53521 atatctaaca tactgataaa ccatttattt gttgtactta tggacttgtt ttatgcagcg
    53581 tggcagtcac gacagcgtta aaggcacgga atgccacgca aaatacaaat tatactcgct
    53641 atacatctcg ccagttgtag ttaacgtaac tggctgtctt tcgaacctct gcgattatat
    53701 cctgtataag ctggtttttg taaatctttc tcctgctgac aatgaataca taagcgcacg
    53761 ccaggaatgg cttcccgacg ggcttgcggg atgggggcac cgcactcttc acattcatac
    53821 aggctttcgc cacgcggaat ttcaccccga gcgcgggcaa tcgcatcttc aattgtactg
    53881 ttgatctgtt cgttgacggc gtcatcgtta gcccaaccgg atgccatatc gacctcccca
    53941 tatcaatact tgtacagtta agtgtagcta atccagggac gaactcgggc agttcaagca
    54001 tcagatctcc gaccattccc gcagcagatt atgataaaga ttaagcagcg acaggatctc
    54061 ttcactttcg ccgtagcggc ttttcagcga ctgaatattg ttgtccagtt caaacagcat
    54121 ggcgcgcttt ttatcatcgc ggatcatcga ctggatccac ataaatgatg ccactcgtac
    54181 gccgcgggtt acgggtgtca cgcaatgcag gctgctggag ggatacaaca cgagatcgcc
    54241 tgccgggagt tttacccgat gttgtccgaa ggtgtcatta acgaccagtt cgccgccgtc
    54301 gtagctttgt ggatcgctta aaaacagcgt ggcagaaagg tcagtacgca tccagccgtt
    54361 ttgtggatgg ctgcgtactg cgccatccac atggaaacca taggtttcat tgttctgata
    54421 gcgattaaac agcggcgtgg aaagggtacg cggcaaggcc gcggcaaaga ataaagcatg
    54481 ttggttaacc gcgttcagca cctcattttg cagggcggcg tataacgtgc tgcgggtgtc
    54541 gacctgttga ttgttcttaa cttgcgcacc ttgtgcgccg gtggtgacgc gtccatccac
    54601 ccattcggct tgttccagtt gttcgcgaaa ccgggcgacg tcctgtggcg ataacacgcc
    54661 gggaatgtgg tacatcatac aagttctcca aaaagtgggg cctgcgcccc acatctgaat
    54721 cagaaatgca tattggctgt gagcaagaag gttcttggct cgcccgggtg ataacggtag
    54781 ccgctcttgt tgattgaggc gacgtaatcg gtatcaaaca ggttgtaaac gtttagctgg
    54841 aagtcgagat tgcgattaac ccgatacccc agtttggcat cggcgaccca gtaaccttcg
    54901 gtaaacgctg gcgttcccac cgcgccgtct gaacctttat gcatactgcc gatatagcgt
    54961 gcgcccgcgc caacagagat atcatcggtt gcctgatact ggc
//
LOCUS       DAEVZA010000014        52910 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEVZA010000014 DAEVZA010000000
VERSION     DAEVZA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.014"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1355
                     /gene="gpmM"
                     /locus_tag="LAF92_000907"
                     /gene_synonym="pgmI"
     CDS             <1..1355
                     /gene="gpmM"
                     /locus_tag="LAF92_000907"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBI9789960.1"
                     /translation="EVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGAVDKAKN
                     AGKAVHIMGLLSVGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPRSAESSL
                     KKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADTAVAGLQ
                     AAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVNADFDGF
                     ARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISETEKYAHV
                     TFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKYDTIICN
                     YPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQMRDPAT
                     GQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGKPLFIVE
                     "
     gene            1365..2648
                     /gene="envC"
                     /locus_tag="LAF92_000908"
     CDS             1365..2648
                     /gene="envC"
                     /locus_tag="LAF92_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBI9789961.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            2652..3611
                     /locus_tag="LAF92_000909"
     CDS             2652..3611
                     /locus_tag="LAF92_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBI9789962.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(3598..4632)
                     /gene="waaH"
                     /locus_tag="LAF92_000910"
     CDS             complement(3598..4632)
                     /gene="waaH"
                     /locus_tag="LAF92_000910"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBI9789963.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(4871..5896)
                     /gene="tdh"
                     /locus_tag="LAF92_000911"
     CDS             complement(4871..5896)
                     /gene="tdh"
                     /locus_tag="LAF92_000911"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBI9789964.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(5906..7102)
                     /gene="kbl"
                     /locus_tag="LAF92_000912"
     CDS             complement(5906..7102)
                     /gene="kbl"
                     /locus_tag="LAF92_000912"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBI9789965.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(7377..8234)
                     /gene="yibB"
                     /locus_tag="LAF92_000913"
     CDS             complement(7377..8234)
                     /gene="yibB"
                     /locus_tag="LAF92_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBI9789966.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            8538..9470
                     /gene="rfaD"
                     /locus_tag="LAF92_000914"
     CDS             8538..9470
                     /gene="rfaD"
                     /locus_tag="LAF92_000914"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBI9789967.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            9480..10526
                     /gene="rfaF"
                     /locus_tag="LAF92_000915"
     CDS             9480..10526
                     /gene="rfaF"
                     /locus_tag="LAF92_000915"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBI9789968.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            10530..11522
                     /gene="rfaC"
                     /locus_tag="LAF92_000916"
     CDS             10530..11522
                     /gene="rfaC"
                     /locus_tag="LAF92_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBI9789969.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            11519..12727
                     /gene="rfaL"
                     /locus_tag="LAF92_000917"
     CDS             11519..12727
                     /gene="rfaL"
                     /locus_tag="LAF92_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBI9789970.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(12763..13905)
                     /locus_tag="LAF92_000918"
     CDS             complement(12763..13905)
                     /locus_tag="LAF92_000918"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBI9789971.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
     gene            complement(13914..14927)
                     /locus_tag="LAF92_000919"
     CDS             complement(13914..14927)
                     /locus_tag="LAF92_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HBI9789972.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEYKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(14952..15659)
                     /gene="rfaY"
                     /locus_tag="LAF92_000920"
     CDS             complement(14952..15659)
                     /gene="rfaY"
                     /locus_tag="LAF92_000920"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBI9789973.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(15685..16692)
                     /gene="waaO"
                     /locus_tag="LAF92_000921"
                     /gene_synonym="rfaI"
     CDS             complement(15685..16692)
                     /gene="waaO"
                     /locus_tag="LAF92_000921"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBI9789974.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDV
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(16735..17532)
                     /gene="rfaP"
                     /locus_tag="LAF92_000922"
     CDS             complement(16735..17532)
                     /gene="rfaP"
                     /locus_tag="LAF92_000922"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBI9789975.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(17525..18649)
                     /locus_tag="LAF92_000923"
     CDS             complement(17525..18649)
                     /locus_tag="LAF92_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBI9789976.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(18646..19704)
                     /gene="rfaQ"
                     /locus_tag="LAF92_000924"
     CDS             complement(18646..19704)
                     /gene="rfaQ"
                     /locus_tag="LAF92_000924"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBI9789977.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            20117..21394
                     /gene="waaA"
                     /locus_tag="LAF92_000925"
     CDS             20117..21394
                     /gene="waaA"
                     /locus_tag="LAF92_000925"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBI9789978.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            21402..21881
                     /gene="coaD"
                     /locus_tag="LAF92_000926"
     CDS             21402..21881
                     /gene="coaD"
                     /locus_tag="LAF92_000926"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBI9789979.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(21920..22729)
                     /gene="mutM"
                     /locus_tag="LAF92_000927"
     CDS             complement(21920..22729)
                     /gene="mutM"
                     /locus_tag="LAF92_000927"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBI9789980.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKYAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(22827..22994)
                     /gene="rpmG"
                     /locus_tag="LAF92_000928"
     CDS             complement(22827..22994)
                     /gene="rpmG"
                     /locus_tag="LAF92_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBI9789981.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(23015..23251)
                     /gene="rpmB"
                     /locus_tag="LAF92_000929"
     CDS             complement(23015..23251)
                     /gene="rpmB"
                     /locus_tag="LAF92_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBI9789982.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(23468..24143)
                     /gene="radC"
                     /locus_tag="LAF92_000930"
                     /pseudo
     CDS             complement(23468..24143)
                     /gene="radC"
                     /locus_tag="LAF92_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            24315..25535
                     /gene="coaBC"
                     /locus_tag="LAF92_000931"
     CDS             24315..25535
                     /gene="coaBC"
                     /locus_tag="LAF92_000931"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBI9789983.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25513..25971
                     /gene="dut"
                     /locus_tag="LAF92_000932"
     CDS             25513..25971
                     /gene="dut"
                     /locus_tag="LAF92_000932"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBI9789984.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            26078..26674
                     /gene="slmA"
                     /locus_tag="LAF92_000933"
     CDS             26078..26674
                     /gene="slmA"
                     /locus_tag="LAF92_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBI9789985.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(26711..27352)
                     /gene="pyrE"
                     /locus_tag="LAF92_000934"
     CDS             complement(26711..27352)
                     /gene="pyrE"
                     /locus_tag="LAF92_000934"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBI9789986.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(27418..28134)
                     /gene="rph"
                     /locus_tag="LAF92_000935"
     CDS             complement(27418..28134)
                     /gene="rph"
                     /locus_tag="LAF92_000935"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBI9789987.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            28261..29124
                     /locus_tag="LAF92_000936"
     CDS             28261..29124
                     /locus_tag="LAF92_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBI9789988.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            29345..30169
                     /gene="dinD"
                     /locus_tag="LAF92_000937"
     CDS             29345..30169
                     /gene="dinD"
                     /locus_tag="LAF92_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBI9789989.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            30461..31078
                     /locus_tag="LAF92_000938"
     CDS             30461..31078
                     /locus_tag="LAF92_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBI9789990.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(31075..32757)
                     /gene="ligB"
                     /locus_tag="LAF92_000939"
     CDS             complement(31075..32757)
                     /gene="ligB"
                     /locus_tag="LAF92_000939"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBI9789991.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            33015..33638
                     /gene="gmk"
                     /locus_tag="LAF92_000940"
     CDS             33015..33638
                     /gene="gmk"
                     /locus_tag="LAF92_000940"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBI9789992.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            33693..33968
                     /gene="rpoZ"
                     /locus_tag="LAF92_000941"
     CDS             33693..33968
                     /gene="rpoZ"
                     /locus_tag="LAF92_000941"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBI9789993.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            33987..36095
                     /gene="spoT"
                     /locus_tag="LAF92_000942"
     CDS             33987..36095
                     /gene="spoT"
                     /locus_tag="LAF92_000942"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBI9789994.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            36102..36791
                     /gene="trmH"
                     /locus_tag="LAF92_000943"
     CDS             36102..36791
                     /gene="trmH"
                     /locus_tag="LAF92_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBI9789995.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            36797..38878
                     /gene="recG"
                     /locus_tag="LAF92_000944"
     CDS             36797..38878
                     /gene="recG"
                     /locus_tag="LAF92_000944"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBI9789996.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPVEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(38863..39729)
                     /locus_tag="LAF92_000945"
     CDS             complement(38863..39729)
                     /locus_tag="LAF92_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9789997.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(39732..40937)
                     /gene="gltS"
                     /locus_tag="LAF92_000946"
     CDS             complement(39732..40937)
                     /gene="gltS"
                     /locus_tag="LAF92_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBI9789998.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            41217..42608
                     /gene="xanP"
                     /locus_tag="LAF92_000947"
     CDS             41217..42608
                     /gene="xanP"
                     /locus_tag="LAF92_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBI9789999.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            42729..44438
                     /locus_tag="LAF92_000948"
     CDS             42729..44438
                     /locus_tag="LAF92_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBI9790000.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(44491..46809)
                     /gene="yicI"
                     /locus_tag="LAF92_000949"
     CDS             complement(44491..46809)
                     /gene="yicI"
                     /locus_tag="LAF92_000949"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBI9790001.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(46819..48201)
                     /locus_tag="LAF92_000950"
     CDS             complement(46819..48201)
                     /locus_tag="LAF92_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBI9790002.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            48494..48588
                     /locus_tag="LAF92_000951"
     tRNA            48494..48588
                     /locus_tag="LAF92_000951"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48528..48530,aa:Sec,seq:tca)
     gene            48667..48828
                     /locus_tag="LAF92_000952"
                     /pseudo
     CDS             48667..48828
                     /locus_tag="LAF92_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            49225..50409
                     /locus_tag="LAF92_000953"
     CDS             49225..50409
                     /locus_tag="LAF92_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HBI9790003.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            50520..51443
                     /locus_tag="LAF92_000954"
     CDS             50520..51443
                     /locus_tag="LAF92_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI9790004.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(51447..52265)
                     /gene="nlpA"
                     /locus_tag="LAF92_000955"
     CDS             complement(51447..52265)
                     /gene="nlpA"
                     /locus_tag="LAF92_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBI9790005.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            52487..52780
                     /locus_tag="LAF92_000956"
     CDS             52487..52780
                     /locus_tag="LAF92_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790006.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT        13640 a        13422 c        12540 g        13308 t
ORIGIN      
        1 ccgaagtagg ccacgttaac ctgggtgccg gccgcatcgt gtatcaggac ctgactcgtc
       61 tggacgttga aatcaaagat cgcgctttct ttgctaatcc agtgctgact ggcgcggtag
      121 ataaagcgaa aaacgcaggt aaagcagtac acattatggg tctgctctcc gtaggcggcg
      181 tacacagcca cgaagatcac atcatggcga tggtagaact ggcagctgaa cgcggcgcag
      241 aaaaaatcta cctgcacgca ttccttgacg gtcgcgacac cccgccgcgc agtgctgaat
      301 cctcgctgaa aaaatttgaa gagaaatttg ctgcgctggg taaaggccgc gtagcgtcca
      361 tcattggtcg ttactacgcg atggaccgcg ataaccgttg ggatcgcgtt gaaaaagcct
      421 atgacctgct gactctggcg cagggtgagt tccaggccga tactgccgtt gctggtttgc
      481 aggcagctta tgctcgcgac gaaaacgacg agttcgtgaa agcgaccgtt attcgtgcag
      541 aaggtcagcc agatgcggca atggaagacg gcgatgcgct gattttcatg aacttccgtg
      601 ctgaccgcgc gcgtgaaatc actcgtgcat tcgtgaacgc tgatttcgat ggcttcgcgc
      661 gtaagaaagt ggttaacgtc gatttcgtga tgctgaccga atacgccgct gacatcaaaa
      721 ctgcggttgc ttacccaccc gcttccctgg ttaacacctt cggcgagtgg atggcgaaaa
      781 acgacaaaac tcagttgcgt atttccgaaa ccgaaaaata tgcccacgtt actttcttct
      841 tcaacggtgg cgtagaagag tcgttcaaag gcgaagatcg cattctgatc aactcaccga
      901 aagtggctac ctacgatctg caaccggaaa tgagctccgc agagctgacc gaaaaactgg
      961 ttgcggcaat caaaagcggc aaatacgaca ccattatctg taactatccg aacggcgaca
     1021 tggtaggtca caccggggtg atggaagcgg cggttaaagc ggttgaagca ctggatcact
     1081 gcgtggaaga agtcgcgaaa gcggttgagt ccgtgggtgg acaactgctg atcaccgctg
     1141 accacggtaa cgctgaacag atgcgcgatc cggcaacggg tcaggcacac acggcacaca
     1201 ccaacctgcc agttccgctg atttacgttg gtgataagaa cgtgaaagcg gttgcaggcg
     1261 gcaaactttc tgacatcgcg ccgaccatgt tgtcgctgat gggtatggaa atcccgcaag
     1321 agatgactgg taagccgctg ttcatcgtgg aataatccct ccccatgagg ggaaaggcga
     1381 ttaataccat gacacgggcc gtgaaaccgc gcaggtttgc aatcaggccc atcatctacg
     1441 ccagcgttct tagcgctggc gtattgttgt gcgccttttc cgcccacgcg gatgagcgtg
     1501 accaactcaa atctattcag gccgatatcg ccgcaaaaga gcgcgcggta cgccaaaagc
     1561 aacaacaacg cgcaagcctg ctcgcacaat tgaaaaagca ggaagaagcg atctctgaag
     1621 ccacccgtaa gctgcgcgaa acgcaaaaca cgctcaatca actcaataaa cagattgatg
     1681 agatgaacgc gtcgattgcc aaactggagc agcaaaaagc cgcccaggag cgcagcctcg
     1741 ccgcgcaact ggatgccgca ttccgtcagg gcgagcatac cggtattcag ctaattctca
     1801 gcggtgaaga aagccagcgt ggacagcgtt tacaggctta tttcggctat ctcaaccagg
     1861 cgcgacaaga aaccattgcc cagttgaagc aaacgcgtga agaagtcgcc atgcagcgtg
     1921 ctgaactgga agagaaacaa agcgagcaac aaacgctgtt atatgaacag cgcgcccaac
     1981 aggcgaaact gactcaggcg ctgaacgagc gtaaaaagac gctggcaggg ctggagtctt
     2041 ccatccagca aggtcagcaa cagttgagcg agctgcgcgc caacgaatcc cgtctgcgta
     2101 acagcattgc ccgtgcggaa gccgcggcga aagcgcgtgc agaacgagaa gcacgtgagg
     2161 cccaggcggt tcgcgaccgc cagaaagaag cgacgcgtaa aggcaccacc tacaaaccga
     2221 ctgaaagcga aaaatcgctg atgtcccgta ccggtggtct gggcgcaccg cgtggtcagg
     2281 cattctggcc ggttcgcggg ccgacgctgc atcgctatgg cgaacagcta cagggtgaat
     2341 tacgctggaa agggatggtt atcggtgcct ctgaaggtac tgaagttaaa gcgattgccg
     2401 acggtcgggt gattctggct gactggctgc aaggttacgg tctggtggtg gtggttgagc
     2461 acggtaaagg cgacatgagt ctttacggct ataatcagag cgcactggtg agcgttggtt
     2521 cgcaggttcg cgcgggccag ccaattgcac tggtgggcag cagtggcggt cagggtcggc
     2581 cttcactcta tttcgaaatt cgccgccagg gtcaggcggt caatccacag ccgtggttgg
     2641 gaagataagt tttgtttcca tttcgtcgta acgttcttgc atttgccgct ctgttggcgc
     2701 tctcctcccc cgtacttgct ggcaaacttg ccatcgtcat tgatgatttt gggtatcgcc
     2761 cgcacaacga aaaccaggtg ctggcgatgc cctccgctat ctccgtcgct gtattacccg
     2821 attcaccgca cgccagagag atggcgacca aagcgcataa cagcgggcac gaagtgttga
     2881 ttcatctccc gatggcacca ctgagtaaac agccgctgga gaaaaatacg ctgcgcccgg
     2941 agatgagcag cgacgaaatt gagcgcatta ttcgtagtgc ggtcaataac gtgccctatg
     3001 ccgtggggat caacaaccac atgggtagca agatgacctc taacctgttt ggtatgcaga
     3061 aagtgatgca ggcactggag cgttacaatc tttacttcct cgacagcgta accatcggta
     3121 atacccaggc gatgcgcgcc gcgcaaggta ctggcgtgaa ggtgatcaaa cggaaggtgt
     3181 tcctcgacga ttcgcagaac gaagcggaca tccgtgtgca atttaatcgc gcaattgatc
     3241 tggcgcgtcg caacggttca accattgcca ttgggcatcc tcacccttca acggtacgcg
     3301 tgctacaaca gatggtttat aacctgccgc cagacattac gctggtgaaa gccagcagct
     3361 tgctgaatga accgcaggtt gatacttcta caccgccgaa aaacgctgtg cctgatgcac
     3421 cgcgtaatcc gttccgtggc gtgaagctgt gcaaaccgaa gaaaccgata gaacctgttt
     3481 atgctaatcg cttctttgaa gtattaagcg aaagcatcag ccagagcacg ctgatcgttt
     3541 acttccagca tcagtggcaa ggctggggca aacagcccga agcggcgaag tttaacgcta
     3601 gcgcaaatta aaggcgctac gcgtaatgcg atggtgcgac cgcgttttgt cgcgccattg
     3661 ccataagcgg aaagaccaca gtaacgcctg gtaaccgacc ttcacgctgc gcacattggt
     3721 aatcaggcgc ttatacatac cggaagtaaa tatctcggca atcatccgtt ggcgggtaag
     3781 aatatccggc tctttgcgca ccgcatggca aacacgcaat gcttcgtaag ttatttgctg
     3841 atgaaattcc ggataaatcg taattttgtc ggcataattt cgatttaatt tctccagcag
     3901 gcgggtaatc ttaatatagt gacgttgata attaaggttt ttattccctt gtctatgcaa
     3961 ccgactcact gacgtattat gcagataata tttatataat gattgctcgg tatatcgcgc
     4021 acgcagcgcg ttaaacatga attctgttgt ccagacaata tcctgatgat gtaatccggc
     4081 aataaattta atgttatttt taacaataac atcacggcga taaaccccca tccagacaac
     4141 gtgagtccaa cggcgcgaag aaagccccat ccgcagccag tccgggccgg ttaatacgcc
     4201 ggttgagcga aggcgatcgc tggggatgga ttgccaggtt tctcccgttt cacgaaaaca
     4261 ccagtcagcg ttgcactgcg ccacgtcgag gtcatcctct aacgccatgg tcatcagcgt
     4321 ttcgtacatg gtgggataga cctcatcgtc agcatcgaca aaagcgacat atttgcccgt
     4381 cgccacctcg atcccacgat tacgcgctac cgatgcgcca gcattcgcct gatgcaacaa
     4441 acgaacgtgc ggatagtttt ctgcgtaatg ctttgctatt tcaacagaat tatccgttga
     4501 accatcgtta ataataatga tttccagagc agtccaggtt tgcgtaatta aagattccat
     4561 acaagtgcgg aaatcatcgc ccgcattata taacggaata ataacactaa gtttattggt
     4621 gctgttcatc ataaattacc gacgacctct gaagttatcc tgacgtttta caggctttgt
     4681 attaagaaac tattaagcct gtgagatatt atttttacag gtgagagagt cacttcaggg
     4741 tagagagata atgagagcag ctatataaga gatcagcgca attacgatat ctttattaca
     4801 ctgacaggat tttacatatt taacggatta tcctcaggca aaagggctgg aataccagcc
     4861 cttgttcgtg ttaatcccaa ctcagaatta ctttcccgga ctggcccgaa cgcatagcgt
     4921 caaagccctt ctggaaatca tcgatagaga aacgatgggt aatgatcggc gagagatcca
     4981 gacctgactg aatcagcgcc gccatcttgt accaggtttc aaacatctca cgaccgtaaa
     5041 tacctttaat gaacaagcct ttaaagatta ctttggtcca gtcgatagac atatcggacg
     5101 gtggaatacc cagcatcgca atgcgaccgc cgtggttcat ggtgtcgagc atggtacgaa
     5161 acgctggcgg cgcgccggac atttccagac cgacatcaaa gccttcggtc atgcccagtt
     5221 ctgccatcac atcattaagg ttttctttag cgacgttgac cgcacgggtg atacccattt
     5281 tgcgcgccag ctcaaggcgg tattcgttaa catcggtgat caccacattg cgtgcaccaa
     5341 cgtgtttcgc caccgccgca gccataatac ctatcggacc tgcaccagaa accagcacat
     5401 cttcgcccac cagatcgaac gacagcgcgg tatgcacggc gttaccgaag ggatcaaaaa
     5461 tggaagccag gtcgtcggag atattgtcgg ggattttgaa agcgttgaac gccggaatca
     5521 ccagatattc ggcgaagcag ccaggacggt taacaccgac accaatggtg ttgcggcaca
     5581 gatgggtacg accgccacga cagttgcgac agtgaccgca ggtgatatgg ccttcgccag
     5641 aaacgcgatc accgattttg aagcctttca cttcctgacc aatacctacc acttcaccga
     5701 catattcatg gcctacgacc atcggcaccg ggatggtttt ttgcgaccac tcatcccagt
     5761 tatagatgtg aacgtcagtc ccgcagatgg ctgttttacg gattttaatc agcagatcgt
     5821 tatgcccgag ttccggtaca ggaacgtcgg tcatccagat gccctcttcc gctttcagtt
     5881 tggataacgc tttcatctca catcctcagg cgataacgcc cagttgttta ccaatacgcg
     5941 tgaatgcttc tactgcacgc gtaatttgct caggggtatg cgccgcagac atctgggtac
     6001 gaatacgcgc ctgacctttc ggaacgaccg gatagaagaa accggtgacg taaatgccct
     6061 ctttttgcag ctcacgggca aatttctgcg ccactactgc atcgccaagc atgaccggaa
     6121 taatggcatg atcggctccc gccagggtaa agcccgccgc cgacatttgc tcacggaact
     6181 ggcgcgcgtt cgcccacaga cggtcacgca gttcgctgcc cgcttcgacc atctccagta
     6241 ctttgatgga cgcggcaaca atggccggtg ccagcgagtt ggagaacagg tacggacgag
     6301 aacgctggcg cagccactca accacctctt tgcgcgccgc ggtataacca ccagaagccc
     6361 cgcccagcgc tttaccaagc gtaccggtga taatatcgac ccggcccatc acatcgcagt
     6421 attcatggga accacgacca ttttcaccga caaaaccgac cgcgtgggag tcgtctacca
     6481 tcaccagggc atcgtattta tctgccagat cgcaaacgcc cttcagattg gcaatcacgc
     6541 cgtccatcga gaatacacca tcggtggcaa tcagcacatg acgcgcaccg gcttcacgcg
     6601 cttctttcag acgagcttca agctcctgca tatcgttgtt ggcatagcga tagcgtttag
     6661 ctttacacag acgcacacca tcaataatag acgcgtggtt cagtgcgtcg gagataatgg
     6721 cgtcttccgc gcccagcagc gtttcaaaca ggccaccgtt agcatcaaag caggaagagt
     6781 agagaatcgc atcttccatc cccaggaagg ccgccagttt ttgttcaagc tctttatggc
     6841 tgtcctgagt gccgcaaata aaacgcaccg aagccatgcc gaaaccgtga gaatccattc
     6901 ccgcctttgc cgccgcaatc agatcaggat gattcgccag cccgagatag ttgttggcac
     6961 aaaagttaat gacgtggctt ccatcagcca cagtgatatc tgcttgctgc gcagacgtaa
     7021 taatgcgctc ttctttaaac aacccttccg cccgtgcggt ttccagatcg ttggttaact
     7081 gctgataaaa ttctccacgc attgcgattc tccagacagg gcaaattcca gcacatatta
     7141 cccaaactta taggtcgcga cgagatgacg cgttaacact tctgcaaaat tcaggataaa
     7201 taacgtgtaa cttgatgtct ctcaacacct acagatagta gaaaaactgt acagttttgg
     7261 ctgggcataa tgatgattga ttttactgca ttgatataaa aagaaaaaat gatgccaaag
     7321 catcattttt cctatctcag ttggtatcaa aacgctaccc actaatggag ggaaacctat
     7381 tttcttgaaa ggaaaattct cagcgcctgc atttttgccc ctaaagtgtt actcctgaag
     7441 cagcgaaaca aatcgaacca ttttcctctc cccagatagt ttaggttgaa gagatcgggg
     7501 cgtttgtaat agcacatgac aaaaatgccc tggtcatcat ccacaatgtt attatttagt
     7561 gttatttttt gactttccag aactaattta tagaattctt tccatttatg ctgtgaacca
     7621 acaattgcgc ccccaataat atatacatga ttgccaatca tgaaatcaaa aacctgttgt
     7681 tgagatgtga cagtaagccc tttttttata gtgaaaagat gcatcttact ttcatcaaat
     7741 gggaaatccc aaattttcaa tcctcgagtt acatttggct tacggcaata gccaaaatct
     7801 atccacgcaa caagaggcgt tttcactaag cccatgttga tagctttatt tacaaaatag
     7861 gccttaaggt tacataccaa aacatactct ggtgaccagt actctgggtt ttttaattgt
     7921 cgaggctcaa gtctatttgt aaacgactca tctttttgaa ttttttcgat acgacttctg
     7981 atatatctaa atttcttttt tatatcgata acgataaccg ttgttggttt tccgttgcga
     8041 atcgcttcaa cccttggttt caaatcggga gaagtgaaaa taatcatctc attttcaagt
     8101 gcggccagac gctcaaagta actaaaataa acatcaactg aacgagctag tttttcacgg
     8161 aaccctttat tagcagtcca atcccctcta ccaatgtcaa aatatgcagt gataattgtg
     8221 gtggatgatt tcattactaa taagaccatt aatataaaaa aagaattata tcataaatat
     8281 taacagaatg tatactgtat attatccatt caaatgcttc cgttatttat catacatcgg
     8341 aatattgata ctaaagcact attcacatgc aaaaccaaca tccgccatga aggactagct
     8401 aaaacccaaa ctagtttgtt gcaattagca tccttgcacc tctatgtaaa gggctgaagg
     8461 gattcggatg tgatggtatg attacagaca ttcgtgtctg agattgtctc tgactccata
     8521 attcgaaggt tacagttatg atcatcgtta ccggcggcgc gggctttatc ggcagcaaca
     8581 tcgttaaagc cctgaatgat aaaggcatca ccgatattct ggtggtggac aacctgaaag
     8641 acggcaccaa gtttgtgaac ctggtggatc tgaatatcgc agactatatg gataaggaag
     8701 acttcctgat ccagattatg gctggcgaag agttcggcga tgtcgaagcg attttccacg
     8761 aaggcgcgtg ctcttccacc accgagtggg acggcaagta tatgatggat aacaactatc
     8821 aatactccaa ggagctgctg cactactgcc tggagcgtga aatcccgttc ctgtacgctt
     8881 cttccgcagc cacctacggc ggacgcacct ccgactttat tgaatcccgc gagtacgaaa
     8941 aaccgttgaa cgtctacggt tactcaaaat tcctgtttga tgaatatgtt cgccaaatcc
     9001 tgccggaagc gaactcgcag attgttggct tccgctattt caacgtttat ggaccgcgtg
     9061 aaggccataa aggcagcatg gcgagcgtcg ctttccatct caacacccag cttaataacg
     9121 gtgaatcacc gaagctgttc gaaggtagcg agaactttaa acgcgacttc gtctatgttg
     9181 gcgatgtggc agatgtaaac ctgtggttcc tggaaaatgg cgtttccggc atcttcaacc
     9241 tcggcaccgg tcgtgcggaa tccttccagg ctgtagctga tgctacgctg gcttatcaca
     9301 agaaaggcca gatcgaatac attccgttcc cggataagct gaaaggccgc taccaggcgt
     9361 tcactcaggc agatctgaca aatctgcgcg cggcgggtta cgacaaaccg ttcaaaaccg
     9421 ttgctgaagg tgtaacggaa tacatggcct ggctgaatcg cgacgcataa gagctctgca
     9481 tgaaaatact ggtgatcggc ccgtcttggg ttggcgacat gatgatgtcg caaagtctct
     9541 atcgcacgct ccaggcgcgc tatccccagg cgataatcga tgtgatggca ccggcatggt
     9601 gccgtccatt attatcgcgg atgccggaag ttaacgaagc tatccctatg cctctcggtc
     9661 acggagcgct ggaaatcggc gaacgccgca aactgggtca tagcctgcgt gaaaagcgct
     9721 acgaccgcgc ctacgtctta cctaactcct tcaaatccgc attagtgcct ttcttcgcgg
     9781 gtattcctca tcgcaccggc tggcgcggcg agatgcgcta cggtttactc aacgatgtac
     9841 gcgtgctcga taaagaagcc tggccgctaa tggtggaacg ctatatagcg ctggcctatg
     9901 acaaaggcat tatgcgcaca gcacaagatc tgccgcagcc attgttatgg ccgcagttgc
     9961 aggtgagcga aggtgaaaaa tcatatacct gtaatcaatt ttcgctttca tcagaacgtc
    10021 cgatgattgg tttttgcccg ggtgcggagt ttggtccggc aaaacgctgg ccacactacc
    10081 actatgcgga gctggcaaag cagctgattg atgaaggtta tcaggtggtt ctgtttggct
    10141 cggcgaaaga tcatgaagca ggcaatgaga ttcttgccgc tttaaatacc gagcagcagg
    10201 catggtgtcg gaacctggcg ggggaaacac agcttgatca agcggttatc ctgattgcag
    10261 cctgtaaagc cattgtcact aacgattctg gcctgatgca cgttgcggcg gcgctcaatc
    10321 gtccgctggt tgccctgtat ggtccaagta gcccggactt cacaccgccg ctatcccata
    10381 aagcgcgcgt gatccgtctg attaccggct atcacaaagt gcgtaaaggt gacgctgcgg
    10441 agggttatca ccagagcttg atcgacatta ctccccagcg cgtactggaa gaactcaacg
    10501 cgctattgtt acaagaggaa gcctgacgga tgcgggtttt gatcgttaaa acatcgtcga
    10561 tgggcgatgt tctccatacg ttgcccgcac tcactgatgc ccagcaggca atcccaggga
    10621 ttaagtttga ctgggtggtg gaagaagggt tcgcacagat tccttcctgg cacgctgccg
    10681 ttgagcgagt tattcctgtg gcaatacgtc gctggcgtaa agcctggttc tcggccccca
    10741 taaaagctga acgcaaagcg tttcgtgaag cgctacaagc agagaactat gacgcagtta
    10801 tcgacgctca ggggctggta aaaagcgcgg cactggtgac acgtctggcg catggcgtaa
    10861 agcatggatt ggactggcaa accgctcgcg aacctttagc cagcctgttt tacaattgta
    10921 agcatcatat tgcaaaacag cagcacgccg tagaacgcac ccgcgaactg tttgccaaaa
    10981 gtttgggcta tagcaaaccg caaacccagg gcgattatgc tatcgcacag cattttctga
    11041 cgaacctgcc tacagatgct ggcgaatatg ccgtatttct tcatgcgacg acccgtgatg
    11101 ataaacactg gccggaagaa cactggcgag aattgattgg tttactggct gattcaggaa
    11161 tacggattaa acttccgtgg ggcgcgccgc atgaggaaga acgggcgaaa cgactggcgg
    11221 aaggatttgc ttatgttgaa gtattgccga agatgagtct ggaaggcgtt gcccgcgtgc
    11281 tggccggggc taaatttgta gtgtcggtgg atacggggtt aagccattta acggcggcac
    11341 tggatcgacc caatatcacg gtttatggac caaccgatcc gggattaatt ggtgggtatg
    11401 ggaagaatca agtagagtgt cgttctacaa gtatgtctct tgcagatttg ccagctcaaa
    11461 ccgtttttca aaatttaaat cttgaaataa taaccaataa gttgacatcg gagataagat
    11521 gacctcaaca ttatttttct ctctcgagaa aaaaaactgg atagcgtact ggaacagagc
    11581 tctcgtattc ttattcatta ccacctattt tttgggtggg attacaaggt ataaacatct
    11641 tattgttatt cttatgacaa taacgacaat cgtctatctc tgcaaacggc caaaacacta
    11701 tctctcacta tttaaaacat ttctttttgg tagtgttgcc atattaacta ttgctgcatt
    11761 gctgtcactt cttcaatccc ctgatgcagg tgctagcatg aaggaaattt tcaaagctat
    11821 tattgagaat actttactat gcacaatagc aataccgatc atattgagag acgagaaaag
    11881 agaagatgtc gaaaaaatcg ttttcttctc atttattagt gcgttgggct tacgctgttt
    11941 ttctgaattg attacctatt ataaggacta tcaacaaggg ataatgccat tcgcagatta
    12001 tagacaccgt agcatttctg actcgatggt ctttttattc cctgcattgt taaatctctg
    12061 gcttatcaaa tcagcaaaat accgcatttc ttttgtggtt ctaagcgtta tttttatttt
    12121 tctgatatta ggaactttat ccagaggggc ctggctttcc gtgttagtca ttggattaat
    12181 atggattctg atgtttaaac aatggaagtt actattagta ggagtaatgg ttgccatcat
    12241 tgcattgtcg gttattttca cacataagga gatgaccgca aagctaacgt ataaacttca
    12301 acaaactaat agttcttatc gctatgcaaa tggtactcaa ggcagcgcac tcgatctaat
    12361 attagaaaat cctgttattg gttatggtta cggtaacgtt gcatataaag atgtctataa
    12421 taaacgtgtc attgattatc cagaatggac ctttagacaa tcaatagggc cacataattt
    12481 tgcgctattc atctggtttg gcactggttt attagggctg gtaagtctta tgatgctata
    12541 ctgtgcaata ttgaaagagt gtataaaaaa tggcgtcaag aataaatatc gctcaccata
    12601 taatgcatat tatataatct tactatcttt tataggttat tttgttatcc gtggaaacgt
    12661 agaacaaatt gaaccaaatt tattaggcgt ttacgccggc ttattattag cgatgaaaaa
    12721 caagtaaaaa ataaaaaagg ctgcataatg cggccttttt atttattcaa accaattatg
    12781 aataacctct tcgaatcttt gagttacgcc atcccaactg tagtgttcaa acacaaagtc
    12841 ctgaccttgc tttgcaactg ctgttaattc aggattcgct aacgttttta aaatatcgct
    12901 gcttatggag tctgcagtca taggttcttt taaatggaat cccgtagtat tttctttgac
    12961 aaattccgtc atccccccac gcgtactcac taaaacaggc ttaccagcac ccatagcttc
    13021 aattgccacc atgcaaaatg gttcctggaa ctgggaagga atgacgacta aatctgccag
    13081 tgggtaataa caatgcatct tttcaggcgg tatgcttcct aacataatgc aacgatcctt
    13141 caaacgtttt gcaatttccc tgacattgcg ttgatatgca cctttgtcac ttttactcat
    13201 ttctgtataa tcaccaatca caactaattt taaatttttg tgagcagcgg ccaatttttc
    13261 gaatgattgc atcaacagca atatgccttt gtcagggacg atacgcccag cataaaaaat
    13321 aattttctct tcaggggaga tattaagttc gcttctagtg atcggctgaa aattagattg
    13381 ataggtttct aaatcaattc catttggtac aatctcaata tctgcatttg ctaaataaga
    13441 ttggtaatac tttttcaagt ataaactagg cactatcatt ttaacatttt gctcaagcaa
    13501 ttttggttca aatgcattat gcatatgtat aaccactctt gcctgaggag ctcgcttacg
    13561 aatttgagtg tatagcttca tgctattatg aactacaata acactttctt tcgttatagg
    13621 aaaatcatgt gcaatattca atattctctg tgaatatgga agaggatcaa gtcttgtcca
    13681 tttttgaaat aaccgcttat atatgcgact gaatccaacc ctatgaatac tacaattatc
    13741 attaacaaag gtgtaatttg agtatcctgg atttttgata caaacaatac gatttggaaa
    13801 attggtacgc tgagcaacct gatacatcca cgtttcaaca gcagccgctc cgcgaggagg
    13861 aattgaaaaa ataggagtaa ccgtaaatat tattttatca accatctcgt atattaacct
    13921 ttcataacat tatatttaat tgctgtaaaa acaccatcga gaaatttttt ctggtaaagc
    13981 aagtgtttat atttttcttt gtactcatgt tttttaacag cttttttata aggtatattt
    14041 ctccatggtg atatattata aatattacga aaataatctg cagaggcgta gttcgcccaa
    14101 tcatgccatg gcttagttat accagtataa tgtatgaaga cagtgtcatc attaataaac
    14161 cgggtgtaat attcactatt tttttcttca aattctgact ttatagtata aatacaatta
    14221 tattttctcg gtaatatttt agcatgatgt agaaacatta agtttaaaat atcttgatcc
    14281 gggtacttta atttttttat aattgactca tctgataata gatcaaagaa tttttccgtt
    14341 agtctttgtt ttaaccattc ccgtaaatta atatacatta caccagagtt aaaatagcta
    14401 ccgttaaaat cctcattaca caaacgctca gcgcttttac tttgcatagc atctacatca
    14461 attaccactg ccccatattc atctttaaac tctaatgcta ttaactcgtt caatgaaccc
    14521 ttacagacga tatcagcatc aagatataat aatgaatcca atcgcgcaga aaaataatca
    14581 aatgaaagca aacgataata aatagatact ggccagatat ttgattgagg tagcgccttc
    14641 aatggctcag aatcaatatg atataattta attactgttt gatacttcgt tgctaattgt
    14701 gataataatt caatatactc tttgtctaag taatcagcga ttatgtgaaa ggtaaatttg
    14761 ttttgtttat tattttcaag aattgaagca attgaaatcg cagccccaac ctggtagttc
    14821 tcatcaatac cccatgagac attaaacgtt tcccgttcat caagtttaac ggggcgcttg
    14881 tccagttcaa ttatatcttt aaattgagta agatgtttaa aatccaattt aattaccctt
    14941 catcataatc attatttgct ctttaatcct agtttgactt ttacatccct gattactttt
    15001 ttaattttct ttttgaatat aagatatgta tagccaaagt ctttcaattc attttttata
    15061 ttatagtgcc gttcaagatc aatgcgatct tttgctttta aaacagcggt tgttttttta
    15121 cctgaaaggt caattagtct taaacctttt tcggatacta taaaatttcc tttatgagga
    15181 tcgccagaca ccattccatg ctgatgcaac tcttttatcg attctgataa ttgatccttc
    15241 agatcttcag aaatttccaa atactcattt aacccgacac cttcaatata ctcaatgagc
    15301 ataatataat aatgagcgaa atttaatgtt ttacgttcag ctagcaaaaa atagtcatta
    15361 attgattgga tgccttcacc acgtacccga tcggtttgat aaattaaatt ttcataataa
    15421 tcttttttta tgcaagattt taagaaacgt tcggtcattt tatgctttgg cgcataaact
    15481 ttaagtacaa gaggtccacg agcagtgtca ataagaatga ctttagtatc atcaatcgaa
    15541 cgaaaaactt ttaatgtttt aaccctgcaa gttaaaaagt catttagaac ttgctcataa
    15601 aagggatcat tatcttttat gaaaactttc aacccattta tcgttttatt atatatcata
    15661 atgaattatt ttaaccttaa atctttagaa gcatttttct ttataatact ttaaataatt
    15721 aaaaatgcct gccatataat gcttttgttt aaatttatgt tttgcacaat accgatactg
    15781 attgctattt acaggtttaa gtaaatcttc tttactccac ggagaagctg ctttggcgat
    15841 caaaaaacta cgtgacaccg gatagtcagc ccactcatgc cagggttttg taggtcccac
    15901 atagtgtata aacacggtgt catcattgac tggattatca actttgtctt ttaattcata
    15961 gttaatacta tatcgggtat tatatttctc cgaaataaac ttcactttac cattcaataa
    16021 tacattcagt acatcttgat caaggtgggt gattttactt acccaatctg ggtcacgcaa
    16081 catttcaata gcttttgacg aaatgttatt tagattccac tcatcaatat ttatcagtaa
    16141 aaaaccagca ttgaaatatc cagaagctaa ctgtggtgta gttaataccg aggctcgatt
    16201 ttgccaccat tcaacatctc tttcagctac aactgcggca atttcattag tagaaaattg
    16261 ataatctaag agttctttaa tactaccctt gcaagcaata tctgcatcaa gatatagtat
    16321 tttttcatgt ttatgataga aataatctgc aattataaat cgaaaatatg ttgcgtaagt
    16381 ccagtttttc gtgcttggta atgacttcag cttatcacaa ttgataacat aaatattaat
    16441 tcttgaatta tattgttttg ctaaatcaga aaaatataat ttgtctttat cactgatata
    16501 atctgtgaaa acatgaaatt cacaagagat ttctctattg tttaatagaa tagatgcgat
    16561 ggctacacca catccaaaaa gaaaatttct gtcaatacca aatgctatat caagagtatt
    16621 tttagatctt atattttgat aatgaaaatt atactcaaaa aggatgatgt cactatcatt
    16681 gagttgagac atatttattc tgtatccaaa attttggcaa aaataagtta cgggttacaa
    16741 cgattttcga atcgttcttt ccctgatttt tgtggctttt gcttctgctt gcgacagcag
    16801 tccttgttcc tgcttgagaa tgtctttaag cggggcggca aaatacactt tcataaaccg
    16861 ccagatatcc cgctgagtca ggccgatatt catcgaagaa aaataaagcc caataagatc
    16921 tttatcccgc caacgacgtg gaacgcgcgt gcgaagctgc gcccggtgca ggtcaattac
    16981 cgaaattttt aactcctctt ccttaccgga aaaaggcaag tgcagcagga aatgacagat
    17041 ataacagtca cggtggttaa tgcccgcagc atgcatatcg cgcaccatcg tcgcgacacg
    17101 cttaataagc atacgcttta cgcgaacatc tggagggtta gtcgcccagt cagcactgta
    17161 atcttcaaga cttatggttg gtgtcagatc ttcggtaata ataaatgaag ttctggtcag
    17221 cggattcatg cctttttcgc caaatgccac cccatacata gtatcaacgc cgacatcccg
    17281 cagtcgatga atcgcattcc attcgcggtc agcgcctaat actggcatcc gcaatgagag
    17341 taaatttttg attatctctt tcagggtcgt gccgcgatgc catttgagaa aatagctttt
    17401 gcccgccatt tcaaagcgca gagtacggcg agtttccagt tcacgaaata cctcaccctg
    17461 caaggtttta acttcctcaa aaggatcttt gccgcgccat aacgtggcaa acggctcttt
    17521 aagttcaacc atccagacca cccgttatga tatccgccgc tttctctggc agactgtata
    17581 aatcttgtgt atcagcataa tgtcgcgcat tttccgccca ggcctggcgc aatgaagatt
    17641 gcgttaacgc tttgcgtaaa atctcattca atgtttcctg gcggaatggc tcagcaatag
    17701 cctcgccgca attagcgtcg acaatataat gcgcatagcc acaaacggct gttgttagta
    17761 ccggtaatcc tgcagtaatc gcttccagca gcacaattcc cgccgcttcc tggtaggcag
    17821 gatgcagtaa taaatccgcc gccgccatta attccgagac atcattgcgc cccgagaaaa
    17881 agtgaacatt actgcgcacg ccgcgttttt ctgccagcgc ctcaaatttt cgcggtttat
    17941 cctgcccaac aacatatagc aatgtgttgt ggcgcagcga atccggtaac gaagcaagtg
    18001 cttcaatgga acgatcgaca cctttacgcg tgaagtctga accgacctgc aacaataaat
    18061 attgttgttc ggttattcca ttcttcttac ggaagatttc acggctattg gctggctgct
    18121 ggctatattt acgatcagga taaatccccg gtggcagaat atgaaaacgc tccgcttcag
    18181 tctgataatg tttctggaaa tcggcgattt gcttatctgt cagcatcagc agctgtgtcg
    18241 gcttgccctg ttcgaaggtt gcccgctcaa aggcggcata atggcgataa cgtgacgtca
    18301 ggcgatagaa aaagcctttt tcctgcgcta ctttctcggc ataacaaaca tcagcggcat
    18361 aataaacgtc cagccccggc attttgttga atccaacgac tttatcgacg ggatgttcgc
    18421 gtaaatgttt ttgcacccag gcaaaatact ccgcattgcg cccgtgattg gtatgcgatt
    18481 taaccggcac tttgatcagt tcaaatacat cagggcattc gccttcccac gactgggtat
    18541 aaacccgaac atgatgacct cgggcggcga ctgtctgagc aatacgcata aaatcacgct
    18601 gcaaaccgcc aaagggaaaa tatttatata aacaaaaagc aacgatcata acgacgtacc
    18661 tgtcgtggag gagggcagta atttatcgac agcagcaatg acatctgccg ccggaataac
    18721 cgaaagatac atctcatttc ggtcacgctg atcgcgcgtt ggcatttccc ggtaatctcc
    18781 cgcccagaat tgaatcatat tatttgacca gggacgccag aaaatatggt ctgtcgcacc
    18841 aaacagcgat atcagcggcg tattaactgc agcggcaata tgcgccggtg cggaatcaac
    18901 gccaataaac agctgcgcat gatcgattaa cgcaccaagt tccgggaagg tcacctttcc
    18961 agccagcgcc gttactggtg gcgtctggca tccctgcgca atttcattga cgcaggccag
    19021 atcgtcttta tctgggccgg acgtcagaac gacttcataa ccacgagcat gtaaggcatc
    19081 aatcacagcg gaaaacttgg cgttgtccca gcatttgaag atttgccgcg ccgtaggttg
    19141 gataaccaca taattttgtc caacaccagc gtgatcaagt tcgcgacgca tacgtttcca
    19201 gcttgcaggc gggtaactca tggttgtctg cttcaccaac gaatcaagtc ccaatggtgt
    19261 cagcacggat aagttacttt ccaccacatt tccaccctgc aacggcacca aatgggtgaa
    19321 acttttacgc caaaaagcag actgccgatg atgataatcc tgggaaattt tcacacgggc
    19381 atttaataag cgaaccagta tagcaaccat ccattgatcg gtaagattga cgataaggtc
    19441 atacttattg gcacgtaata ccttgatgag atgaaaaaag ttggcaattt tttctgaggc
    19501 ttttgctttt ttatttttta tgccgtagag cgcgttaatc tctggatttt cagacaggat
    19561 cgggatggtg tcctgataaa gcagcacatc gatttttgcg tcagggtaat tttttttcag
    19621 cgaactaatg acgggagtag ttaataacat atccccatga aaacgcatct taatgagcaa
    19681 aattcttcga aatggcttat ccacaagtaa ctctttttgt tgagattatc cgattaaggt
    19741 gaagcagaaa aagcacgcta ccgccccagg ctcaacagct acctgaatac tgataccgca
    19801 ctgatattga cgcgctagtg tatcacttct ttcgcggcaa tccgacccga atgtttttta
    19861 agcacattgt tgattttcaa taaagaaatc aatcaaataa aatcattagc aaccatattc
    19921 tttcactgtt gctctgcgat tccttcctgc acgtacagca gaaatcaatg aatcgcataa
    19981 aaatacaaaa aaagtataga tgttcagcaa gaattggcgg gtaaattgct atttaagtca
    20041 aaaacgggaa aagtaatggt aaagccacag ctaaatacat agaatcccca gcacatccat
    20101 aagtcagcta tttactatgc tcgaattgct ttacaccgcc cttctctacc ttattcagcc
    20161 gctgatctgg atacggctct gggtgcgcgg acgtaaggct ccggcctatc gaaaacgctg
    20221 gggtgaacgt tacggttttt accgccatcc gctaaaacca ggcggcatta tgctgcactc
    20281 cgtctccgtc ggtgaaactc tggcggcgat cccgttggtg cgcgcgctgc gccatcgtta
    20341 tcctgattta ccgattaccg ttacaaccat gacgccaacc ggttcggagc gcgtacaatc
    20401 ggctttcggg aaggatgttc agcacgttta tctgccgtat gacctgcccg atgcgctcaa
    20461 ccgtttcctg aataaagtcg accccaaact ggtgttgatt atggaaaccg aactatggcc
    20521 taacctgatt gcggcgctac acaaacgtaa aattccgctg gtgatcgcta acgcgcgact
    20581 ctctgcccgc tcagccgcag gttatgccaa actgggtaaa ttcgtccgtc gcttgctgcg
    20641 tcgtattacg ctgattgctg cgcaaaatga agaagatggt gcacgttttg tggcgctggg
    20701 cgcaaaaaat aatcaggtga ccgttaccgg tagcctgaaa ttcgatattt ctgtaacgcc
    20761 gcagttggct gctaaagccg tgacgctgcg ccgccagtgg gcaccacacc gcccggtatg
    20821 gattgccacc agcactcacg aaggcgaaga gagtgtggtg atcgccgcac atcaggcatt
    20881 gttacagcaa ttcccgaatt tattgctcat cctggtaccc cgtcatccag aacgcttccc
    20941 ggatgcgatt aatcttgtcc gccaggctgg actaagctat atcacacgct cttcagggga
    21001 agtcccctcc accagcacgc aggttgtggt tggcgatacg atgggcgagt tgatgttact
    21061 gtacggcatt gccgatctcg cctttgttgg cggttcactg gttgaacgtg gtgggcataa
    21121 tccgctggaa gctgccgcac acgctattcc ggtattgatg gggccgcata cttttaactt
    21181 taaagacatt tgcgcgcggc tggagcaggc aagcgggctg attaccgtta ccgatgccac
    21241 tacgcttgca aaagaggtgt cctctttact caccgacgcc gattaccgta gtttctatgg
    21301 ccgtcatgcc gttgaagtac tgtatcaaaa ccaaggcgcg ctacagcgtc tgcttcaact
    21361 gctggaacct tacctgccac cgaaaacgca ttgaggttgt tatgcaaaaa cgggcgattt
    21421 atccgggtac tttcgatccc attaccaatg gtcatatcga tatcgtgacg cgcgccacgc
    21481 aaatgttcga tcacgttatt ctggcgattg ccgccagccc cagtaaaaaa ccgatgttta
    21541 ccctggaaga gcgtgtggcg ctggcacagc aggcaaccgc gcatctgggg aacgtggaag
    21601 tggtcgggtt tagtgatttg atggcgaact tcgcccgtaa tcaacacgct acggtgctga
    21661 ttcgtggcct gcgtgcggtg gcggattttg aatatgaaat gcagctggcg catatgaatc
    21721 gccacttaat gccggaactg gaaagtgtgt ttctgatgcc gtcgaaagag tggtcgttta
    21781 tctcttcatc gttggtgaaa gaggtggcgc gccatcaggg cgatgtcacc catttcctgc
    21841 cggagaatgt ccatcaggcg ctgatggcga agttagcgta gcgtttatgc cggatggtat
    21901 gccatccggc gcgcatgaat tacttctggc actgccgaca ataaaacgtt gcccgctgcg
    21961 catgtttagt cgccacaatc ggcgtaccgc acacccgaca cggctcacct tttcgcccgt
    22021 aaacctgcaa ttcctgagcg aaatagcccg gtttaccatc actttgcaga aaatctttca
    22081 gcgttgtacc accctgctca atcgaacgca gcaacaccgc tttaatcacc cgagctaaca
    22141 attcacactc tgccagcgac agtgatgacg ccagccgatc cggatggatc cccgccgcaa
    22201 acagtgattc gctggcatag atattcccta cccctaccac cagcttgtta tccatcagcc
    22261 acggtttaat cgccgttttt ttcttcgcgt acttctgatg cagatactca ccattgaaat
    22321 cgtcgctaag cggctccggt ccaagatggg ccagcacatt atgcccttcc agctctttgg
    22381 tccacagcca ggcaccaaag cggcgcggat cggtgtagcg cagcacttta ccgttgctca
    22441 tcaccaaatc cacatggtca tgcttttcag ggggaagttc ttctggaagg atgcgcaggc
    22501 tgccagacat ccccaaatga atgataatcc agccctcagg cagctccagc agcaaatatt
    22561 tagcccgccg ctgcacgcta agcactggtt ggtcgcttaa acggtagatc tcttctgaaa
    22621 ccggccagcg caagcgtccg ttgcgcacca ctgcatgaag aatggttgca ccaacgagat
    22681 gcggttctat gccgcgacgg ctggtttcaa cttcgggtaa ttcaggcata gcatctccag
    22741 gaatgaacag atgcaatcaa tatgggggca agcagataac aaaaaacccc gccggagcga
    22801 ggttttttgt tacatcaaag cgagaattat ttgattttcg cttctttgta gatcacgtgc
    22861 tgacgaacaa ctggatcgaa ttttttcagt tccagttttt ccggcttagt acgtttgttc
    22921 ttcgtagtgg tatagaagtg accagtacca gcagaagaaa ccagcttgat tttctcacga
    22981 atacctttag ccatgattta tttcctctaa gtacttagta cttttcgcca cgggcacgca
    23041 gttcagccag aactgtatcg atgccttttt tatcgattac acgcatacct ttagcagata
    23101 cgcgcagggt gacaaaacgc ttctcgctct caacccagaa acggtgagag tgcaggttcg
    23161 gcaggaaacg gcgtttagtc gcgttcagtg cgtgggaacg gttgttaccg gtcaccggac
    23221 gcttgccagt aacttggcag actcgggaca tgtctattct ccaaaaatca aattagctcg
    23281 agcttcgtat ggggtattgg cgcctcgtca ggctttacag cccggtcatc gcagttctat
    23341 gtgaactctc gattgccagg cccaaatgcc aaacccgaga ttctcaaagg tggcgtagta
    23401 tacgctgact cagcgatgtg ctcaagtccc gaacagacaa agatcccgaa ggatcgcgca
    23461 tagcgggtta aatccagccg cgttcggcaa aagaaacata ctctccacgc ccaatcacga
    23521 tatggtcgag cacgcgtaaa tccatgaact gacaactctt tattatccgt tcagtaatga
    23581 gtttatccgc tttactgggt tcagcacaac ccgaagggtg attatgtgca aggatcagcg
    23641 ccgaggcgtt tatttttatc gcttcgcgga taatttcccg aggatggact tcaacatggt
    23701 ttagcgtgcc ggaaaaaaga cggctatgcg ttataacccg gtgttgggag tcgagaaaga
    23761 tcaccataaa gatctcccgc tcctcacccg tgagctggct ttgtaaaaat tccctcgtca
    23821 tctccgggct gagtaaaggg ctttcttcac gcatccgcac gttgtagtaa cgccgcgcca
    23881 gttcagcaat cccctttaac tgggcaaatt tcgccacgcc aattccatga acgccactaa
    23941 attgttcata ttcagaggtt aacaagccat aaagagagcc gaaattctcc agcatctctt
    24001 ttgccagggt taatacatct ttaccgcgcg ttccggtttc cggtacgcag aaatagcgcc
    24061 agcagctcga catccgttaa ggcgctaata ccaaacttca gcattttttc gcgcggcatc
    24121 aacagctgtg aattgttttt caccttcacc tcctttgtgg tgctcgcatc ctgtcatagc
    24181 cgttccgtta aatcgacggc cagttttcaa tcctggaaag cgcctcgcaa agtgattctc
    24241 aggtgaaggc acgacaacaa aagtattgtg ataaaatcgc caacttctgg tgtcacccaa
    24301 caggaaaaat catcatgagc ctggccggta aaaaaatcgt tctcggcgtt agcggcggta
    24361 ttgctgccta taaaacccct gaactggtgc gtcgtttgcg cgatcgcggg gccgacgtcc
    24421 gcgtagccat gaccgaagcg gcaaaagcct ttatcacccc acttagcttg caggcggttt
    24481 ctggttatcc cgtttccgac agtctgctgg acccggcagc cgaagccgct atgggccata
    24541 ttgagctggg taaatgggct gatttagtga ttctcgcccc tgccacggca gatttgattg
    24601 cccgtgttgc tgccggaatg gcgaatgacc tggtatcgac gatttgtctg gctacacctg
    24661 cgcctgtagc cgtgctcccc gccatgaacc agcagatgta ccgtgccgct gccacgcagc
    24721 ataatttaga ggtgcttgct tcccgtggtt tgctcatctg ggggccagac agtggcagtc
    24781 aggcttgtgg tgatatcggt cctgggcgaa tgctcgatcc gttaaccatt gtggatatgg
    24841 cggtagcgca tttttcgccc gtcaacgacc tgaaacatct gaacattatg attaccgccg
    24901 gcccgacgcg tgaaccgctc gatcccgtgc gttatatctc taatcacagc tccggcaaga
    24961 tgggttttgc tatcgccgcc gccgctgccc gtcgtggcgc gaacgtcacg ctggtatcag
    25021 gtccggtttc actaccgacg ccaccgtttg ttaaacgtgt tgatgtgatg accgcgctgg
    25081 aaatggaagc cgccgtgaat gcttctgtac agcagcaaaa tatttttatc ggctgcgccg
    25141 ccgtggcgga ttatcgcgca gctaccgtgg ccccagagaa aatcaaaaag caggccacgc
    25201 agggtgatga attaacaata aaaatggtta aaaaccccga tatcgtcgca ggcgttgccg
    25261 cactaaaaga ccatcgaccc tacgtcgttg gatttgccgc cgaaacaaat aatgtggaag
    25321 aatacgcccg gcaaaaacgt atccgtaaaa accttgatct gatctgcgcg aacgatgttt
    25381 cccagccaac tcaaggattt aacagcgaca acaacgcatt acaccttttc tggcaggacg
    25441 gagataaagt cttaccgctt gagcgcaaag agctccttgg ccaattatta ctcgacgaga
    25501 tcgtgacccg ttatgatgaa aaaaatcgac gttaagattc tggacccgcg cgttgggaag
    25561 gaatttccgc tcccgactta tgccacctct ggctctgccg gacttgacct gcgtgcctgt
    25621 ctcgacgacg ccgtagaact ggctccgggt gacactacgc tggttccgac cgggctggcg
    25681 attcatattg ccgatccttc actggcggca atgatgctgc cgcgctccgg attgggacat
    25741 aagcacggta tcgtgcttgg taacctggta ggattgatcg attctgacta tcagggccag
    25801 ttgatgattt ccgtgtggaa ccgtggtcag gacagcttca ccattcaacc tggcgaacgc
    25861 atcgcccaga tgatttttgt tccggtagta caggctgaat ttaatctggt ggaagatttc
    25921 gacgccaccg accgcggtga aggcggcttt ggtcactctg gtcgtcagta acacatacgc
    25981 atccgaataa cgtcataaca tagccgcaaa catttcgttt gcggtcatag cgtgggtgcc
    26041 gcctggcaag tgcttatttt caggggtatt ttgtaacatg gcagaaaaac aaactgcgaa
    26101 aaggaaccgt cgcgaggaaa tacttcagtc tctggcgctg atgctggaat ccagcgatgg
    26161 aagccaacgt atcacgacgg caaaactggc cgcctctgtc ggcgtttccg aagcggcact
    26221 gtatcgccac ttccccagta agacccgcat gttcgatagc ctgattgagt ttatcgaaga
    26281 tagcctgatt actcgcatca acctgattct gaaagatgag aaagacacca cagcgcgcct
    26341 gcgtctgatt gtgttgctgc ttctcggttt tggtgagcgt aatcctggcc tgacccgcat
    26401 cctcactggt catgcgctaa tgtttgaaca ggatcgcctg caagggcgca tcaaccagct
    26461 gttcgagcgt attgaagcgc agctgcgcca ggtattgcgt gaaaagagaa tgcgtgaggg
    26521 tgaaggttac accaccgatg aaaccctgct ggcaagccag atcctggcct tctgtgaagg
    26581 tatgctgtca cgttttgtcc gcagcgaatt taaataccgc ccgacggatg attttgacgc
    26641 ccgctggccg ctaattgccg cgcagttgca gtaatatgac gccggatgac ttttcatccg
    26701 gcgagtttct ttaaacgcca aactcttcgc gataggcctt aaccgccgcc agatgttccg
    26761 ccatttccgg cttctcttcc aggtaagcaa tcaggtcttt cagggtgatg atagagatca
    26821 ctttgcagtt gtaatcacgc tcaacttcct gaatcgccga aatctcgctg cgcccccgtt
    26881 cctgacgatc gagcgaaatc aacacgccag caagcgtcgc gccattagcc tgaataatct
    26941 ccatcgattc gcgaatcgcc gttccggcgg tgatcacatc atctaccagc attacgcgtc
    27001 cttgtaacgc gctaccaacc agattgccgc cttcaccgtg gtcttttgcc tctttgcggt
    27061 taaagcagta cggcaggtca aggtcatgat gctccgccag cgccacggcg gtagtggtgg
    27121 caatcgggat ccctttgtaa gcagggccaa acagcagatc gaactcaagg ccggaatcca
    27181 ccaacgcttc agcgtaaaaa cggcctaaca gtgccagatc gcgcccggta ttaaacagcc
    27241 cggcgttgaa gaaatagggg cttttgcgcc cggacttcaa cgtaaactcg ccaaacttta
    27301 acacctgctt gccaagcgca aattcaataa actggcgctg atatggtttc atgccttcgc
    27361 tcctcatctt acttttctac agacaaaaaa aaggcgactc atcagtcgcc ttaaaaatca
    27421 gtttgccagc gccgccttct gcgtcgctac aatggattcg attccccctc gggccagagc
    27481 caacaaggtg agtaattctt cgtgggtgaa cggttcgcct tctgccgtcc cctgcacttc
    27541 aatgatgcgc ccgtcttcgg tcatcactac gttcatgtcg gtctctgcgg cagagtcttc
    27601 aacgtattcc agatcgcaaa ccgcttcgcc gttcacaatt ccgacagaaa ctgcggctac
    27661 catccctttc atcgggttgg ttttcagctt gccgttttcc accagcttct gtagcgcatc
    27721 tgccagcgcc acgcaggcac ccgtaatcga cgcggtacga gtgccaccat cagcctgaag
    27781 cacgtcgcag tccagcgtaa tggtgaactc acccagcgct ttcaaatcta ctgccgcgcg
    27841 aagagcacgg gcgatcagac gctggatttc cattgtgcgt ccaccctgct tacctttcgc
    27901 cgcttcacga gcgttacggg tgtgggtaga acgcggcagc ataccgtact ctgcggtgat
    27961 ccagccctgg ccctgacctt tcaggaagcg cggcacgcct tcttcaatag aggcggtaca
    28021 caacactttg gtatcgccaa attcgaccag caccgagcct tctgcatgtt ttgtatagtt
    28081 acgagtcagg gtaacgggac gcacctgatt attgctacgg cctgctggac gcatattgaa
    28141 atctccggct tgaaacaaat gtggctgcgc attatacgga cttccggcgg ttattcctat
    28201 cctgacaagg catcgatggc tataatcctt ccacctctcc ttttataaac aggaacgtct
    28261 atgatccgca gtatgaccgc ctacgcccgg cgtgaaatca agggtgaatg ggggagcgca
    28321 acctgggaaa tgcgctcggt aaaccagcgt tatctggaaa cttactttcg tctgccggag
    28381 cagttccgta gccttgaacc tgtcgttcgc gagcgtattc gttctcgcct gacgcgcggt
    28441 aaagtggaat gtaccctgcg ctatgagcca gatgttagcg cacaaggtga gctgatcctc
    28501 aatgaaaaac tggctaaaca gctggtaact gccgcgaact gggtaaaaat gcagagtgac
    28561 gaaggggaaa tcaacccggt tgatattcta cgctggccgg gcgtgatggc agcccaggag
    28621 caggatctcg acgccattgc cgctgaaatt ctcgcggcgt tggatggtac gctggacgac
    28681 tttatcgtcg cgcgcgaaac cgaaggtcag gcgctgaaag cattgatcga gcagcgtctg
    28741 gaaggcgtca ccgccgaagt ggtcaaagtc cgcgcccata tgccggaaat cctgcaatgg
    28801 cagcgtgagc gtctggtcgc gaagctggaa gatgctcagg ttcagttgga aaacaaccgt
    28861 ctggagcagg agctggttct gctggcacaa cgaattgacg ttgccgaaga actggaccgc
    28921 ctggaagcgc atgtcaaaga gacctacaac attctgaaga aaaaagaagc cgttggtcgt
    28981 cgtctggact ttatgatgca ggaattcaac cgcgagtcga acactcttgc gtcgaaatct
    29041 atcaatgccg aagtgacaaa ctccgccatc gagctgaaag tgttgattga gcagatgcgc
    29101 gagcagattc agaatattga gtaactcgta acactaattc ttacttttca gccttcacaa
    29161 atgccgccca ctcaaacaga gcggcatttt tcttccccgc aacttcaatt ctgttttgcg
    29221 tgcctgctcc agattttgcg atgttttttt gcccagcaca ctgagaacgt gagatactca
    29281 caactgtata taaatacagt tacagattta ctttctttgc aatcgatatc acatggagtg
    29341 ggcaatgaac gaacatcatc aaccttttga agaaatacgg cattatggca ctgaagggca
    29401 agaattttgg tcagctagag aattggcacc gttactggat tatcgggact ggcgcaattt
    29461 tcaaaaggta ctggcccgcg cgactcaagc gtgcgaagcc agcaatcaag ctgcatctga
    29521 ccatttcgtt gaaaccacca aaatggttgt attaggttca ggggctcaac gagaacttga
    29581 agatgttcat ctctcccgct atgcctgtta cctggtagta caaaacggcg accctgcgaa
    29641 accggtcatt gcggcagggc agacttattt tgctatccag acccgacggc aggagcttgc
    29701 tgacgatgaa gcatttaagc aacttcgtga agacgaaaag cgtctgtttc tgagaaatga
    29761 gttgaaagaa cataacaaac aattggttga ggcagcacag caagctgggg tagcgacagc
    29821 tactgatttc gccatatttc agaatcatgg ttaccagggg ctgtatggtg gattagatca
    29881 gaaagccatc caccaactga aggggctgaa aaagagtcag aagatcctgg atcatatggg
    29941 ttcaacagaa ctggcggcta atctctttcg agctacccaa acagaagaaa aactcaagcg
    30001 ggatggcgtg aattcaaaac agcaagctaa taccacacac tttgacgtgg gtagcaaggt
    30061 gaggcaaacc attcaggaac ttggcggaac catgcctgag gagttgccga ccccgcaggt
    30121 cagcatcaag cagttggaaa acagtgtaaa aattacagag aagaaatagc agaaactaaa
    30181 aactcttttt gttgattgag atacccgatg cgtaagccaa agtccaggtg cggtgaacac
    30241 aatggctaaa cattgcacct ttcttttcca cccctcaatt ttaacctatt ttttcttatg
    30301 cattttctca gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac
    30361 ataactacaa gttatcaatt tcctctcacc ttaaaaaatc tcaatcgtga caatacgcac
    30421 aaatcgctac cctgccagac agatttttag ggagagaacc atgctgttac acattttgta
    30481 tttggttggc attactgccg aagccatgac gggggctctg gcggccgggc gacgccgcat
    30541 ggatacattt ggcgtaatta ttattgcgac cgccaccgca attggcggag ggtcagtgcg
    30601 cgatattctg ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat
    30661 cgtcgctacc gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa
    30721 agtgtttctg gtgctcgatg cgcttggact ggtggtcttt tctatcatcg gcgcacaggt
    30781 tgcactggat atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggcgt
    30841 gtttggcggc gttttgcgcg atatgttctg taaacgtatc ccactggtat tccagaaaga
    30901 gttgtatgcc ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt
    30961 ttctaaccat gatgtggtaa ttatctccac cctggtattc ggcttttttg cccgcttact
    31021 ggcgctacgc cttaagctgg gattaccggt cttttactac agccacgaag gacactaagg
    31081 ttcaaaacct gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt
    31141 ttccttccat tcaataacct gtctggcgcg ccccgatcca gtccccggca gctgctgcca
    31201 gaactgctcc gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc
    31261 cgcccgggtt agcggtattc ccattgccat cacccagcga gtaaaaggct gcttacgagc
    31321 cagattaaac tgatgccata gctgcgcact tttacttttc gcgatccccg gcgtgttctg
    31381 taattgctct ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg
    31441 cagcgcgcgc cagccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag
    31501 ccagactaag cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa
    31561 cgagttaaag cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac
    31621 atcatcaatg cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc
    31681 aatatcccac tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc
    31741 atccagcatg acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc
    31801 aaactgaatc gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag
    31861 ccattctgcc tggcctggta gccaatggcg ggattcgggt tctttcgccc ctcgcacaac
    31921 cacgccatcg gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac
    31981 ctcatcagca tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc
    32041 cagctccttt aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac
    32101 gcccagagaa ttcagcgtgt cgctatcgtc ctggcgcatc atcaggccag caacttttgc
    32161 gcgggcattt attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc
    32221 cccctgaagc gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa
    32281 gctaactttc tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt
    32341 cagtttcccg tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca
    32401 aagatcgcta cgttctcgca tccacagact taatgcgttt ttatccacca ttttgcgtac
    32461 acccgtatgg gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg
    32521 gggctcgctc ccaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac
    32581 accgtcttcc acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg
    32641 ctgttgcagg cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc
    32701 ccacacagat gattgccagc acaagatact tattaatatc gccatccata ctttcatcat
    32761 cacctccgct gtaggtagtc aggcagatat acaacgtgat gaaagcaaag ccgagtggca
    32821 aaaacggagt ctgcgaggac gcttcctgaa aatcgtcttt attgcagtga atgacaggca
    32881 aatgcggaag cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta
    32941 cgtcacatgg ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca
    33001 tacgaaagat actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg
    33061 gtaaatccag cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt
    33121 ctgtttcaca caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct
    33181 ttgttaatca tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag
    33241 aagtttttgg taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg
    33301 gtgtcgatgt ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaaaatgc
    33361 cgcacgcgcg gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac
    33421 gcggtcgcgg tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag
    33481 aaatgagcca ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt
    33541 tgaccgattt gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc
    33601 gtcatgacgc tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc
    33661 agtcatttct tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg
    33721 tagagaaaat tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga
    33781 tgcaggtagg cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg
    33841 cgctgcgcga aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc
    33901 aggaacagca agagcaggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc
    33961 gtcgttaatc acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc
    34021 aaacctacct gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg
    34081 atgctcacga ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg
    34141 ttgcctgcat tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc
    34201 atgacgtgat tgaagatact cccgccacct accaggacat ggaacagctt tttggtaaaa
    34261 gcgtcgccga gctggtagag ggggtgtcga aacttgataa gctcaagttc cgcgataaga
    34321 aagaggcgca ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg
    34381 tcattctcat caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc
    34441 cggacaaacg tcgccgcatc gcccgtgaaa ctctcgaaat ttatagcccg ttggcgcacc
    34501 gtttaggtat ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc
    34561 ccaatcgtta ccgcgtaatt aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga
    34621 tgatccaaaa aatcctctct gaaatcgaag ggcgtttgca ggaagcggga ataccgtgcc
    34681 gcgtcagtgg tcgcgaaaag catctttatt cgatttactg caaaatggtg ctcaaagagc
    34741 agcgttttca ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattctgaca
    34801 cctgttatcg cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga
    34861 aagactatat cgccattcct aaagcgaacg gctatcagtc gttgcacacc tcgatgattg
    34921 gcccgcacgg cgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg
    34981 agatgggtgt tgccgcgcac tgggcttata aagagcacgg cgaaaccagt actaccgcac
    35041 aaatccgcgc ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt
    35101 cgtttgaatt tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca
    35161 caccggaagg gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg
    35221 cagtgcatac cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc
    35281 cgctgtcgca gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc
    35341 gcccgaatgc cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc
    35401 agttgctgaa aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc
    35461 atgctttggg tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc
    35521 tggatcgcat gaagctggca acgcttgacg atctgctggc agaaatcggc cttggtaacg
    35581 caatgagcgt ggtggtcgcg aaaaatctac aacatgggga cgcctccatt ccaccggcga
    35641 ctcaaagcca cgggcatctg ccgatcaaag gcgctgatgg cgtgctgatc acctttgcga
    35701 aatgctgccg ccccattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc
    35761 tggtgatcca ccatgaatcc tgccgtaaca tccgtggcta ccagaaagag ccagagaagt
    35821 ttatggctgt ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg
    35881 agatgttcaa tcatcagggt gcgctggcaa acctgacggc ggcaattaac accacgacct
    35941 cgaatattca aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc
    36001 gtctgaccgc ccgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc
    36061 cagacgtgat taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg
    36121 cacgcatctg cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg
    36181 tccacaaacc tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg
    36241 aagttcacgc cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta
    36301 gtaacagctg ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca
    36361 aaggccaggg catgcagatt ctggcaaccc atctttctga taacgctgtc gatttccgcg
    36421 aaattgatta cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc
    36481 aggaagcatt ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt
    36541 cgctgaatgt ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa
    36601 atgcaggcat gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt
    36661 tgtttgaagg cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc
    36721 cccacgtcaa tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg
    36781 ctgcagggta agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac
    36841 gggcgttggc gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga
    36901 tctactctta caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga
    36961 actactgccg ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt
    37021 cggcggtcgc cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg
    37081 ctttttcaat ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc
    37141 ttatggcgaa gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt
    37201 gcagggcgat ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac
    37261 ggaaggcgta aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga
    37321 cacctgcgcc attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc
    37381 ggaagcgttg cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac
    37441 cgggcagcat ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag
    37501 catgttagcc ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga
    37561 tgcgctgaaa aataaactcc tcgcagcctt accgttcaag ccaacgggcg cacaggcacg
    37621 cgtagtggcg gagatcgagc gcgatatggc gctggatgtg ccgatgatgc gcctggtaca
    37681 gggcgatgta ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc
    37741 ccacggcaaa caggtggcat tgatggcacc aaccgaatta ctcgccgaac agcacgccaa
    37801 taacttccgc aactggtttg aaccgctcgg tatcgaagtg ggctggctcg ccggtaagca
    37861 gaaaggcaaa gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat
    37921 tgttggtacg cacgccatct tccaggaaca ggtgcagttt aacggtctgg cgctggtgat
    37981 tatcgacgaa caacatcgct ttggcgtgca tcagcgtctg gcgttatggg agaaaggcca
    38041 gcagcaaggc ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct
    38101 ggcaatgacg gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg
    38161 cacgccagtg actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt
    38221 gcgccacgcc tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga
    38281 gtcggaattg ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct
    38341 accagagttg aacgttggcc tggtacacgg gcggatgaaa cctgtcgaga aacaggcggt
    38401 gatggcgtcg tttaaacaag gtgagctaca cctgctggtt gccacaaccg ttattgaagt
    38461 cggcgttgat gtgcctaacg ccagtctgat gattatcgaa aacccggagc gtctgggtct
    38521 ggcgcagtta caccagcttc gcgggcgtgt aggtcgtggt gcggtggctt ctcactgcgt
    38581 gctgctctac aaaacgccgc tctccaaaac cgcgcaaatt cggctgcaag tgctgcgcga
    38641 cagcaacgac ggttttgtga ttgcacagaa agacttagag attcgcggac cgggtgaact
    38701 gttaggtacg cgtcagacgg gtaatgctga gtttaaagtg gcggatttac tgcgcgatca
    38761 ggcgatgatc ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca
    38821 agcaaaagcc ctgatagaac gctggatgcc ggagacggaa cgttactcca acgcatgacc
    38881 agactcatca caagacaagt cacgcagtaa atcttcaatc tggatagtga ggatatccat
    38941 tgcctcatac gcatcctgtg caattcgcgc cgccattgcg ccttccggtt caccacgcag
    39001 aatttcagcc gctgcctgta cgccagccac aatcatttca ggtgttaaca tttggcgaag
    39061 aaaagcgcga ttatcagagg accagccatc tttatggcct aatggatgcc cccagcctat
    39121 ccattcttgc gaccataacg gattggcttc tatgggataa attagatgcg cggcatactg
    39181 aatagcacac accgcttttc ctggcgtatc cagcgcccac cagcgcgacc agagagaacg
    39241 aattaacggc gcggccccac ccagagtatt gaacaccgcc agccagcaaa gaacgttatt
    39301 gaaaccacgc tcgttatcca tacgctgcaa cgcggtgtcc agtaaaaatt gccgtacctg
    39361 ctggcgttgg gaagcattca tcatctcctg ccagagatag ggccgacaca atgccttcat
    39421 taaggattct tcgaagtgat caccataacc gccagctaaa acggtttcat gccattgcgc
    39481 caggcagagc ggaaacagat agcgaaacaa ctctatttgc atcggttcgt tataaacgag
    39541 attgagtaca tagtaagcac tcaaatccca ttcgctgatc tgctgccact ctttagccgc
    39601 cagcgccttg agttcggcat ctgcgtaatc aaattccgtt tcccatacac agagcactgg
    39661 cggctcatca ccaaaaatat ggcgacagtg ttcacgtagc gtttccggtt ccttccgacg
    39721 ctttttcatt tttaaccggc aaaaatcggc aacattaaat acagcttaat caccagcgca
    39781 ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc catgtgcgac
    39841 gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt tgcaccgagg
    39901 ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc catcatgcgc
    39961 caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag aatcgccagc
    40021 atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcagcgc catcgccagg
    40081 aacaacgaca ggcttacgtt acccagcacg gataccgcac gctcaaagac gcggtaaaag
    40141 cccatcattg acagaccgtt gctcagaatc acgccaacaa acagtacaca gacgaaggtc
    40201 ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac cgtcaggcag
    40261 atagcaatca gcgcgatagt ttcaatcagc accagcgagg tgatcatgcg gcccacatcc
    40321 ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg cgtggtggag
    40381 tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac cagaccgaac
    40441 gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc aatgaacaat
    40501 ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc ggccaacagc
    40561 cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc attttgcatc
    40621 accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc ggcacgcaaa
    40681 ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa cggatcgcgc
    40741 agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag tagcgccagc
    40801 gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa aaaggagacg
    40861 gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag cgttgctaaa
    40921 gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat actcgtcata
    40981 cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg ctttcctggg
    41041 tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa ataccaaaag
    41101 ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg gcaaacgttt
    41161 gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg ttttcgatgt
    41221 ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc gcagactcaa aacagcgaac
    41281 tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc gcctgtcagc
    41341 atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc caggcgctgg
    41401 gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc tccggtgtgg
    41461 catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg tctattcagg
    41521 gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg aaaaccggtg
    41581 gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg gcaagttgca
    41641 ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg ccgctggttt
    41701 ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggttgggtta acgtccattg
    41761 gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat ctgctgctgg
    41821 caggcgtggt cttagcctta attatcctgc ttaaccgtca acgtaaccct tacttacgcg
    41881 tggcctcact ggtgattgcg atggcggccg gatatgcgct ggcgtggttt atgggcatgt
    41941 tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg ccgctctatt
    42001 acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt atgatcactt
    42061 cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag ccagtgtccg
    42121 gtccgctgta catgaaacgc ctgaaaggtg gcgtgctggc aaacggcctg aactcgtttg
    42181 tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac ggagtgatcc
    42241 agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg ttgatcgtgc
    42301 tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg gttctgggcg
    42361 gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc gtttctcgtg
    42421 agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt ggtctgggcg
    42481 tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac ctgctctcct
    42541 ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc ccaccagaaa
    42601 aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct ctttcaccat
    42661 tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat ttcacgggat
    42721 ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct ctgttagtgg
    42781 tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat atcagcgcat
    42841 gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac cgtttttccg
    42901 cgccatctca tatcgtgctg gagaacgtca cgtttggccg tgatggccag cccgcgaccc
    42961 tggtggcaaa aagtgtcgac attgcgctaa gcagtcggca actgaccgaa ccacgccatg
    43021 tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc gcgccgctac
    43081 cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg aatagcgaat
    43141 ggaaactgag cgcgcagcgg gtaaatggcg gcgtagttcc gtggtcacca aaagccggta
    43201 aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg ctcaatgatg
    43261 ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt acgctgacta
    43321 acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt aacgccgacg
    43381 gcagctggca agtggaaaac ctgcgcatgg cggatatacg tctacaaagc gaaaaatcgc
    43441 taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt cgcctggaag
    43501 tgatcgatgc tcgtttgcaa ggtccggact gggcggtgac cgacctcgat ctcagcttgc
    43561 gcaacatgac cttcagtaaa gatgactggc agacacaaga aggcaaactg tcgatgaacg
    43621 ctagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac gcggaatttt
    43681 ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt atggtcagaa
    43741 cgtcagggaa ctggctgcgt gacgggaaaa cgttgatcct tgatgatgcg gcaattgccg
    43801 ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg acacccggtt
    43861 ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc attgatatcg
    43921 accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac ctgacgctgg
    43981 ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc gccgccgcga
    44041 cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac agcagcacgg
    44101 tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc accgccagtg
    44161 tttcacaaac gccacaacgt cagacacata tcagcctgaa tggacgcggt gtgccggtga
    44221 atattttgca acagtgggga tggcctaaat taccgttgac tggcgacggc aatattcagc
    44281 ttaccgccag tggcgatatt caggccaatg tcccgttgaa acctacggtt agcgggcaat
    44341 tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgct ggcatcgttt
    44401 ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg ccgggtacga
    44461 ctcagacgta cccggcattc catcaataga ttacaacgta attgtcagcg cattcccttg
    44521 aggcttcacc accagcccct gctcactttc agcctgcgaa ccgtcttgca gaccatttac
    44581 tttcacaaca ttgcgcaggc acagcgtcca gttcttcgcc tcgcccgcac cagtcacagt
    44641 aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt cagtagcggg
    44701 cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg ccgtgccttc
    44761 gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtaggg tgttatcacg
    44821 cacataaatg ggcagactca ggaagctgtg ctgctgttta tgccagcgac taccgtcgag
    44881 ctcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact gcacatcgcc
    44941 cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt gacggtcaag
    45001 gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca tcggcgtacc
    45061 ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc atttcagttg
    45121 cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca cacgataaga
    45181 tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc agcgtttgta
    45241 aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga agccaaaacc
    45301 tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt agttagcgta
    45361 acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg agcgggcaaa
    45421 caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca gttcgttgta
    45481 gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc actgaacatc
    45541 agttgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg cgaccagacc
    45601 tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat aaatcgccag
    45661 acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga gtaaatagcc
    45721 tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt taatccagac
    45781 gcagattttc agtcctttcg ctttcaggcg gcggatcatc ccttccgggt cagggaaagt
    45841 cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac agtcaaagtg
    45901 gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt ttaccgtcgc
    45961 ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc acgcgggcgg
    46021 cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg gcgtcgggcc
    46081 gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt tggagacttt
    46141 ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc cataaccacg
    46201 gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc cgccgtcccg
    46261 gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct ctcccagacc
    46321 gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc gttgattatt
    46381 cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt cgccgttgcg
    46441 cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt tgccactttt
    46501 aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca aaatattgag
    46561 cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca caccgacaat
    46621 accttcctgt ggggagaaaa agcgcaacgt aaataaagga gtatcaagct gccaggtacg
    46681 ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct gttcaacctc
    46741 gaacacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt ttccatcgct
    46801 aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt gctggcaacg
    46861 gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg tgagtgagta
    46921 gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa tcgtgaatag
    46981 cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat catatccgcc
    47041 ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt tgagcacaaa
    47101 cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact cgccgtagtc
    47161 gacggtatcg gacatcatta cccactggat aggtgtcacc agttgatgca acacaccaat
    47221 cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga acatcgcgag
    47281 gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac atttccagtc
    47341 ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag tggtgagaaa
    47401 agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca tcgccccacc
    47461 gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc gccactggtc
    47521 gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg gtgcttcaac
    47581 gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca cggaaaggac
    47641 cgcgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat taaccagtgg
    47701 catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac gccaggattg
    47761 cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc agtaagggat
    47821 attgacgacg gtataaagta aggtaagtag ggtgtaagta attgctgcat agatcatttt
    47881 gccgttcata ctgagatctg gcgtgctata ggccagtaca cagacgatcc cgaatggcag
    47941 tgcgccaaac agtacccacg gacgaaattt accccagcga gagcgcgttc gatcggccaa
    48001 caaccccatg caaggatcgg aaatcgcatc cagtgcacga gcgaccaaaa acatggttcc
    48061 gacaaatccg gcaggaatgc caaaaatatc ggtataaaag aacatcatat ataacattac
    48121 gttatcgaaa ataatgtggc tggcggcgtc tcccatgcca taaccaattt tctctttaac
    48181 ggacaacact tcactcttca tctgcttttc cttgatgcta taggggtgct gagaccgata
    48241 acgcattttg taaaccatcc ctgtgagagc gaatattccg atatctggtt attaatttat
    48301 gattcttgtt ttatgtgatc gtggtagcgt taattccgct catatatcat tgtaaaatat
    48361 gggttttata tgaactataa tgctttcgtg ataatacgct gcgtgtatta ggcggaaaaa
    48421 actgatctgg gggatgtaga aactcaagga agtagctata atgcgccccg cctccatgta
    48481 gcaatcgagg cgcggaagat cgtcgtctcc ggtgaggcgg ctggacttca aatccagttg
    48541 gggccgccag cggtcccggg caggttcgac tcctgtgatc ttccgccaaa atgcctctcc
    48601 tgacgttctc caaccccttg taatactgtc aatccagaga ttttctactg ctcaatattc
    48661 aatcggcttc aggaatacct gatcaaaggt tttgtgatgg acgatgagcg actgaagaat
    48721 ccgcctgtcg gttcttctgt cctcgaacgc atccgcgata ttcgcgccag catgcgccgt
    48781 gtttatttgc aggtgatttg cccccctgct gactctcagc caactgtatc gccattaagg
    48841 tgaagatgtt gttttacacg ctccagaaca ccctttttta tacgactatt ctattccttt
    48901 aacagctgga gaaaaaactg acctataaac ctcaccaata atcagactgc catcaagatt
    48961 aatttgttaa tttaatatat caaatcaact tgttttattt ttactatcaa aaatagctca
    49021 ttgccttact ataattaata tataaattcg gtaattaatt cttaacatgc ttttactaat
    49081 aatctcaatt gctggcccta taatattgcg ctagcattgc tttctggttg tatcagcgat
    49141 actcaaaaat tcttaataca atactcattc gactggtact tatttgtaac tcagttatat
    49201 tttttcgccc ggtgattcag aagaatgcaa aaaacggcta ccactccatc aaaaatactt
    49261 gatctcactg ccgcggcatt tttacttgtc gcctttctga cgggtattgc gggcgctctt
    49321 cagactccta ccctaagtat attcctcgca gatgaactga aagcccgtcc tataatggta
    49381 ggttttttct tcaccggtag cgctattatg ggaattctgg tcagtcaatt tctggcaagg
    49441 cactccgata aacaaggcga ccgtaaatta ctgattctgc tatgttgctt atttggagtg
    49501 ctggcctgca cgctttttgc gtggaatcgc aactacttca ttctcctctc aacgggcgta
    49561 cttctgagta gttttgcttc caccgcaaac ccgcaaatgt tcgccctcgc ccgtgaacac
    49621 gccgacagaa caggccgtga gacggtcatg ttcagtacat ttttacgtgc tcagatctcg
    49681 cttgcctggg ttatcgggcc accgctcgct tatgaactgg caatgggatt tagttttaaa
    49741 gtgatgtatc tcaccgcagc catcgcattt gttgtttgcg gactgatagt ctggttgttt
    49801 ttgccatcaa tacaaagaaa tattcctgtc gttacccaac ccgtagaaat tttaccctcc
    49861 acccacagga agcgggatac gcggctactt tttgtggtct gttcaatgat gtgggcggcg
    49921 aataatctct acatgataaa tatgccgcta tttattattg atgaactgca tctaaccgat
    49981 aaactggctg gagaaatgat tggtatcgct gccggtctgg aaattccgat gatgttaatc
    50041 gcaggctatt acatgaaacg tattggcaag cgactattaa tgctcattgc tatcgtgagt
    50101 ggaatgtgtt tttacgccag cgtactcatg gcgacgactc cggcggttga gctggaattg
    50161 caaattctta atgccatctt ccttggtatt ctctgtggta tcggcatgct ttattttcag
    50221 gacttgatgc ctgaaaaaat aggctctgcg acaacgttat atgcaaatac ttcacgcgtc
    50281 ggctggatta tcgccggctc tgttgacgga attatggttg aaatctggag ctaccatgcg
    50341 ttgttctggc tggcgatagg gatgttgggt attgcgatga tttgcctgct gtttattaaa
    50401 gatatttagc tcaccagtct gggctaagaa aaatcggcgt ggcgcaaaca gagccatcct
    50461 cgccattttt gctatcatgc ctgcatacat aaacgacaaa acagtatgca gagggaaaaa
    50521 tgggttccac cagaaagggg atgctgaacg ttctgattgc cgccgtgttg tggggaagtt
    50581 caggggtctg cgcgcaatac atcatggagc aaagccagat gtcgtcgcag tttttgacta
    50641 tgacgcgttt gatattcgcc ggtttgattc tactgacgct gtcatttgtt catggcgata
    50701 aaatcttttc tattattaac aatcataaag atgccattag cctgctgatt ttttccgtgg
    50761 ttggcgcgct aactgtacag ctcacttttt tgctaaccat cgaaaaatcg aacgcagcca
    50821 cggcaacggt gctgcaattc ctctcaccga cgattatcgt cgcctggttc tcactggtgc
    50881 gtaaatcgcg cccgggcatt ctggttttct gcgctatttt gacatcgctg gtcgggactt
    50941 ttttattggt gacacacggt aatccgacgt cattatcgat ctctcctgcc gcgttgttct
    51001 ggggcattgc ctcggcattt gctgctgcat tctataccac ctatccctca acgctaattg
    51061 cccgctatgg cacgttacca gtcgtcggct ggagtatgct gattggcggt ctgattctgt
    51121 tgccttttta tgccagacaa ggaacaaact ttgtcgttaa cggcagtttg attctggcgt
    51181 ttttttattt ggtggtcatt ggtacgtccc tgacatttag tctgtacctg aaaggagcac
    51241 aattaattgg cggtccaaaa gccagcattt tgagctgtgc agaaccatta agtagcgcgc
    51301 tactctcttt gctgttgctg gggatcacgt tcacattacc ggactggctg ggaacgctgc
    51361 tgattctgtc atcggtgatt ttgatttcaa tggattcccg tcgccgcgcc agaaaaataa
    51421 atcgtccggc gcggcatgag tgagaattac cagccaggca ccgcgccacc gttaaaaatg
    51481 gtttctgccg ctttagcgac ttcgggtgat tgataagatt gcagaaattc cttcacgttt
    51541 tctgcattct tattatcttc ccgtgccacc aaaatattca catacggcga attcttatct
    51601 tcaataaata cgctgtcgtg caccggagaa agccctgtct gctgaatgta agtggtactg
    51661 ataatcgcta catcaacttt gggatcatcc agtacgcgcg gaagctgcgc cccctcgagt
    51721 tccataatct gcaaatgacg aggattatcg gttatatcca gcgcggtagg taataaccct
    51781 tttccctctt tcagagtaat tagcttctct ttttgcaaca gtaaaagtgc gcgaccaagg
    51841 ttggtcgggt cgttggggat cgccactgtc gcgccttctt ttatttgcgc cacggttgtg
    51901 atttttttgg aataacccgc catagggaag acaaacgtat ttcccaccgc taccagttta
    51961 taaccatgcg cctgattatc ttgttcaaga aagggtcgat gctggaagac attcgcatca
    52021 agttcgccat gatttgtcgc atcgttcggt agcaacgaac cgctaaatcc taccagttca
    52081 acatcgaggc catatttctc ttttgccacc tttttagcga cttccgcgac atcttgttct
    52141 gcgccattta taacgccaac tttaatgtgt tttgcatcgc tgctactctg gtcgcaacct
    52201 gccagcagaa ttccggccag caataatgcg gcccctgtcc gtagatgatg tgttgtcagt
    52261 ttcatttttt tatcctttta ttaatgatta cctgactata gcggtaaggt cgctgcggtt
    52321 taaaaaacga aacgctatcg ataagaataa aaaggaataa aagtggaata taaagtctgg
    52381 cacttcttac tgaccacgca agcgcgtttc gtacagcatg atgagagcga tgaaagcaaa
    52441 ctacacttat gcttcattcg atacacattt gtaaaaggtt gaatccatga aaccaacgac
    52501 actactgctt attttcactt ttttcgctat gccaggcatc gtatacgccg agtcgccttt
    52561 tagctcactg cagtccgcaa aagagaaaac cacggtatta caagatttac gtaaaatttg
    52621 cacgccacag gcgtcattat cagatgaagc gtgggaaaag ttaatgttgt cagatgaaaa
    52681 caataaacaa catatacgcg aggccatcgt ggcgatggaa cgtaataatc agagcaatta
    52741 ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa gaacagcaac aactactgac
    52801 caacgcttct ttacctgata tcaggatttc ttcattttca cctttgcagt aaccaacaat
    52861 gcagtcagca acatcaatgt gccggacaac atcaacggcg aagtcagacc
//
LOCUS       DAEVZA010000015        52793 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEVZA010000015 DAEVZA010000000
VERSION     DAEVZA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.015"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1151)
                     /gene="rcsD"
                     /locus_tag="LAF92_000957"
     CDS             complement(<1..1151)
                     /gene="rcsD"
                     /locus_tag="LAF92_000957"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBI9790007.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDNWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNK"
     gene            1890..2993
                     /gene="ompC"
                     /locus_tag="LAF92_000958"
     CDS             1890..2993
                     /gene="ompC"
                     /locus_tag="LAF92_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBI9790008.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            3105..4160
                     /gene="apbE"
                     /locus_tag="LAF92_000959"
     CDS             3105..4160
                     /gene="apbE"
                     /locus_tag="LAF92_000959"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBI9790009.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            4234..5298
                     /gene="ada"
                     /locus_tag="LAF92_000960"
     CDS             4234..5298
                     /gene="ada"
                     /locus_tag="LAF92_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBI9790010.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            5298..5948
                     /gene="alkB"
                     /locus_tag="LAF92_000961"
     CDS             5298..5948
                     /gene="alkB"
                     /locus_tag="LAF92_000961"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBI9790011.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     ATTAAGYPDFQPDACLINRYAAGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            6024..7667
                     /gene="yojI"
                     /locus_tag="LAF92_000962"
     CDS             6024..7667
                     /gene="yojI"
                     /locus_tag="LAF92_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBI9790012.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            7885..9531
                     /gene="mqo"
                     /locus_tag="LAF92_000963"
     CDS             7885..9531
                     /gene="mqo"
                     /locus_tag="LAF92_000963"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBI9790013.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(9680..10168)
                     /gene="eco"
                     /locus_tag="LAF92_000964"
     CDS             complement(9680..10168)
                     /gene="eco"
                     /locus_tag="LAF92_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBI9790014.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(10303..10467)
                     /locus_tag="LAF92_000965"
     CDS             complement(10303..10467)
                     /locus_tag="LAF92_000965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790015.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            10576..11070
                     /gene="napF"
                     /locus_tag="LAF92_000966"
     CDS             10576..11070
                     /gene="napF"
                     /locus_tag="LAF92_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBI9790016.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            11060..11323
                     /gene="napD"
                     /locus_tag="LAF92_000967"
     CDS             11060..11323
                     /gene="napD"
                     /locus_tag="LAF92_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBI9790017.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            11320..13806
                     /gene="napA"
                     /locus_tag="LAF92_000968"
     CDS             11320..13806
                     /gene="napA"
                     /locus_tag="LAF92_000968"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBI9790018.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPG
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            13813..14508
                     /gene="napG"
                     /locus_tag="LAF92_000969"
     CDS             13813..14508
                     /gene="napG"
                     /locus_tag="LAF92_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBI9790019.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            14495..15358
                     /gene="napH"
                     /locus_tag="LAF92_000970"
     CDS             14495..15358
                     /gene="napH"
                     /locus_tag="LAF92_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBI9790020.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            15355..15804
                     /gene="napB"
                     /locus_tag="LAF92_000971"
     CDS             15355..15804
                     /gene="napB"
                     /locus_tag="LAF92_000971"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBI9790021.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            15814..16416
                     /gene="napC"
                     /locus_tag="LAF92_000972"
     CDS             15814..16416
                     /gene="napC"
                     /locus_tag="LAF92_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBI9790022.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            16435..17052
                     /gene="ccmA"
                     /locus_tag="LAF92_000973"
     CDS             16435..17052
                     /gene="ccmA"
                     /locus_tag="LAF92_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBI9790023.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            17049..17711
                     /gene="ccmB"
                     /locus_tag="LAF92_000974"
     CDS             17049..17711
                     /gene="ccmB"
                     /locus_tag="LAF92_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBI9790024.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            17753..18490
                     /gene="ccmC"
                     /locus_tag="LAF92_000975"
     CDS             17753..18490
                     /gene="ccmC"
                     /locus_tag="LAF92_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBI9790025.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            18487..18696
                     /gene="ccmD"
                     /locus_tag="LAF92_000976"
     CDS             18487..18696
                     /gene="ccmD"
                     /locus_tag="LAF92_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBI9790026.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            18693..19172
                     /gene="ccmE"
                     /locus_tag="LAF92_000977"
     CDS             18693..19172
                     /gene="ccmE"
                     /locus_tag="LAF92_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBI9790027.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            19169..21112
                     /gene="ccmF"
                     /locus_tag="LAF92_000978"
     CDS             19169..21112
                     /gene="ccmF"
                     /locus_tag="LAF92_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBI9790028.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            21109..21666
                     /gene="dsbE"
                     /locus_tag="LAF92_000979"
     CDS             21109..21666
                     /gene="dsbE"
                     /locus_tag="LAF92_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBI9790029.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            21663..22715
                     /gene="ccmH"
                     /locus_tag="LAF92_000980"
     CDS             21663..22715
                     /gene="ccmH"
                     /locus_tag="LAF92_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBI9790030.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(22750..23397)
                     /gene="narP"
                     /locus_tag="LAF92_000981"
     CDS             complement(22750..23397)
                     /gene="narP"
                     /locus_tag="LAF92_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBI9790031.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            23717..26308
                     /gene="yejO"
                     /locus_tag="LAF92_000982"
                     /pseudo
     CDS             23717..26308
                     /gene="yejO"
                     /locus_tag="LAF92_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(26412..26488)
                     /locus_tag="LAF92_000983"
     tRNA            complement(26412..26488)
                     /locus_tag="LAF92_000983"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26452..26454),aa:Pro,seq:ggg)
     gene            complement(26563..28323)
                     /gene="pbgA"
                     /locus_tag="LAF92_000984"
     CDS             complement(26563..28323)
                     /gene="pbgA"
                     /locus_tag="LAF92_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBI9790032.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(28343..28570)
                     /locus_tag="LAF92_000985"
     CDS             complement(28343..28570)
                     /locus_tag="LAF92_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBI9790033.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            28752..29759
                     /gene="yejK"
                     /locus_tag="LAF92_000986"
     CDS             28752..29759
                     /gene="yejK"
                     /locus_tag="LAF92_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBI9790034.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(29898..30182)
                     /gene="rplY"
                     /locus_tag="LAF92_000987"
     CDS             complement(29898..30182)
                     /gene="rplY"
                     /locus_tag="LAF92_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBI9790035.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(30307..32067)
                     /locus_tag="LAF92_000988"
     CDS             complement(30307..32067)
                     /locus_tag="LAF92_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI9790036.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32216..32911
                     /gene="rsuA"
                     /locus_tag="LAF92_000989"
     CDS             32216..32911
                     /gene="rsuA"
                     /locus_tag="LAF92_000989"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBI9790037.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            32939..34129
                     /gene="bcr"
                     /locus_tag="LAF92_000990"
     CDS             32939..34129
                     /gene="bcr"
                     /locus_tag="LAF92_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBI9790038.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            34462..34806
                     /locus_tag="LAF92_000991"
     CDS             34462..34806
                     /locus_tag="LAF92_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790039.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(34810..36399)
                     /gene="yejF"
                     /locus_tag="LAF92_000992"
     CDS             complement(34810..36399)
                     /gene="yejF"
                     /locus_tag="LAF92_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBI9790040.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILRLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(36401..37426)
                     /locus_tag="LAF92_000993"
     CDS             complement(36401..37426)
                     /locus_tag="LAF92_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9790041.1"
                     /translation="MSRLSPVNQARWARYRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFANDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(37426..38520)
                     /locus_tag="LAF92_000994"
     CDS             complement(37426..38520)
                     /locus_tag="LAF92_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBI9790042.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(38521..40335)
                     /locus_tag="LAF92_000995"
     CDS             complement(38521..40335)
                     /locus_tag="LAF92_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI9790043.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(40417..41973)
                     /locus_tag="LAF92_000996"
     CDS             complement(40417..41973)
                     /locus_tag="LAF92_000996"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBI9790044.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(42154..42720)
                     /gene="mepS"
                     /locus_tag="LAF92_000997"
     CDS             complement(42154..42720)
                     /gene="mepS"
                     /locus_tag="LAF92_000997"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBI9790045.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(43132..43845)
                     /gene="lpxT"
                     /locus_tag="LAF92_000998"
     CDS             complement(43132..43845)
                     /gene="lpxT"
                     /locus_tag="LAF92_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBI9790046.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(43884..44870)
                     /gene="yeiR"
                     /locus_tag="LAF92_000999"
     CDS             complement(43884..44870)
                     /gene="yeiR"
                     /locus_tag="LAF92_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBI9790047.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(44988..46454)
                     /locus_tag="LAF92_001000"
     CDS             complement(44988..46454)
                     /locus_tag="LAF92_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBI9790048.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(46677..47249)
                     /gene="yeiP"
                     /locus_tag="LAF92_001001"
     CDS             complement(46677..47249)
                     /gene="yeiP"
                     /locus_tag="LAF92_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBI9790049.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            47404..47658
                     /locus_tag="LAF92_001002"
     CDS             47404..47658
                     /locus_tag="LAF92_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBI9790050.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(47655..48836)
                     /gene="setB"
                     /locus_tag="LAF92_001003"
     CDS             complement(47655..48836)
                     /gene="setB"
                     /locus_tag="LAF92_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBI9790051.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            49204..50334
                     /gene="fruB"
                     /locus_tag="LAF92_001004"
     CDS             49204..50334
                     /gene="fruB"
                     /locus_tag="LAF92_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBI9790052.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            50334..51272
                     /gene="fruK"
                     /locus_tag="LAF92_001005"
     CDS             50334..51272
                     /gene="fruK"
                     /locus_tag="LAF92_001005"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBI9790053.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            51289..>52793
                     /gene="fruA"
                     /locus_tag="LAF92_001006"
     CDS             51289..>52793
                     /gene="fruA"
                     /locus_tag="LAF92_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBI9790054.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGA"
BASE COUNT        12688 a        13032 c        14333 g        12740 t
ORIGIN      
        1 attttgttac ttatgacagt gcggttcgat tcctgatcgt gaaccagcag gccgagcggc
       61 agcagtgaga ctatctcttc attaattgcc cgtaaaatgc gcaattcgtt attgaccgcc
      121 gtgctgggta gactttctgt actgcggctg gagaaatggc ggaatgtggt atagccaaat
      181 aacgccagcg ccagcaaacc gatgttcagc agcagtggca gcagaatgtt ttgcaacgta
      241 tccagcaata aggtgccata aggaacctgc cagaccaggc gcatatcggt agagttgagt
      301 gccgaggaga tttcaatctt cgtactgtta aagtggatac tgacgctatc cgtcccttct
      361 ttctcattat cattgtttcc cgttgccgtc gcgtctggct caaggcggaa actgtccagc
      421 ggcatacccg gtgggatcaa atcattaatc ggcagatcaa aagccacgac cgttgccaga
      481 tgtcctggct ggttaaaggt agtacgcaag gtaaagtaat gaccgttctg ccaggccagg
      541 cggcgcatgt tagaaaagct ttcgcgttca tcgagggcgt tggcctgttg caacatctct
      601 gcacgacgtg aatcaacaat gtcgctgacg gtcgattctt taaatccgga ggtgagatct
      661 ttaaggggta gggttgagat cagcaccaga ctgttatcct gaccattcag gtaatacatc
      721 gaccacggta cattttctgc gccccacaat gtatccagat aagtggacat ccgctgagtc
      781 atctcaagcg ttgagttgtc gtgagagcca aagattaacg cttccgtttt gcggcgtggt
      841 ttctccagat agtagacatc ctgtttcagg cgcgtctctt gtaaaccttc gccggaggag
      901 ggggaggtcg tcgcggcaat gttgtcgtag atctgccagg tcacgtaacg ccagttatcg
      961 acgcgctttt ggatagcatg ggtaatgtcg acaatctggt aacttttatc tttcagccag
     1021 gcgttaacgg cgctttgtac cattacaccc atcgtcacca gtaacacaat gatcaacagt
     1081 aaaaagaagc gggtaatgct ccccggtagg agtgaaaagc gggtcgtggc cgttgtctct
     1141 ttctgacgca ttcgtgttta tgacctgtta aaacttcgcg aagtgatagg gcagtataaa
     1201 gggtactgtg tgaaattcca gactcttacc tgattgatga ttctctattc tcccgtgata
     1261 tttatcatcc tgcattgggg aaaatagatg tacaagcgcc attttgctgt acaaataacc
     1321 tacaaaaagc ccaacgaaat taattgcaaa taaacgaaga ttgctggaaa ttatgctgat
     1381 gttaattatt tgtgaaatag ttaacaagcg ttatagtttt tctgtggtag cacagaataa
     1441 tgaaaagtgt gtaaagaagg gtaaaaaaaa ccggatgcga ggcatccggt tgaaataggg
     1501 gtaaacagac attcagaaat gaatgacggt aataaataaa gttaatgatg atagcgggag
     1561 ttattctagt tgcgagtgaa ggttttgttt tgacattcag tgctgtcaaa tacttaagaa
     1621 taagttattg attttaacct tgaattatta ttgcttgatg ttaggtgctt atttcgccat
     1681 tccgcaataa tcttaaaaag ttcccttgca tttacatttt gaaacatcta tagcgataaa
     1741 tgaaacatct taaaagtttt agtatcatat tcgtgttgga ttattctgca tttttgggga
     1801 gaatggactt gccgactgat taatgagggt taatcagtat gcagtggcat aaaaaagcaa
     1861 ataaaggcat ataacagagg gttaataaca tgaaagttaa agtactgtcc ctcctggtcc
     1921 cagctctgct ggtagcaggc gcagcaaacg ctgctgaagt ttacaacaaa gacggcaaca
     1981 aattagatct gtacggtaaa gtagacggcc tgcactattt ctctgacaac aaagatgtag
     2041 atggcgacca gacctacatg cgtcttggct tcaaaggtga aactcaggtt actgaccagc
     2101 tgaccggtta cggccagtgg gaatatcaga tccagggcaa cagcgctgaa aacgaaaaca
     2161 actcctggac ccgtgtggca ttcgcaggtc tgaaattcca ggatgtgggt tctttcgact
     2221 acggtcgtaa ctacggcgtt gtttacgacg taacttcctg gaccgacgta ctgccagaat
     2281 tcggtggcga cacctacggt tctgacaact tcatgcagca gcgtggtaac ggctttgcga
     2341 cctaccgtaa cactgacttc ttcggtctgg ttgacggcct gaactttgct gttcagtacc
     2401 agggcaaaaa cggtagcgta agcggcgaag gcatgaccaa caatggtcgc gatgctctgc
     2461 gtcagaacgg cgacggcgtt ggcggttcta tcacttatga ttacgaaggc ttcggtatcg
     2521 gtgctgcagt ttccagctcc aaacgtactg atgctcagaa caccgctgct tacatcggta
     2581 acggcgaccg tgctgaaacc tacaccggtg gtctgaaata cgacgctaac aacatctacc
     2641 tggctgctca gtacacccag acctataacg caactcgcgt aggttccctg ggttgggcga
     2701 acaaagcaca gaactttgaa gctgttgctc agtaccagtt cgacttcggt ctgcgtccgt
     2761 ctgtagcata cctgcagtct aaaggtaaaa acctgggtac catcgctggt cgtaactacg
     2821 acgacgaaga tatcctgaaa tatgttgatg ttggcgcgac ctactacttc aacaaaaaca
     2881 tgtccaccta cgttgactac aaaatcaacc tgctggacga caaccagttc actcgtgacg
     2941 ctggcatcaa cactgataac atcgtagctc tgggtctggt ttaccagttc taatctcgat
     3001 tgatatcgaa caaagggcct gcgggccctt ttttcattgt tttcagcgta caaactcatt
     3061 tttttggtgt actcttgcga ccgttcgcat gaggataatc acgtatggaa ataagcttta
     3121 cccgcgtggc actgctggct gccgcgctct tctttgttgg ttgcgatcaa aaaccacaac
     3181 ccgccaaaac ccacgctact gaagttaccg ttcttgaagg caaaaccatg ggtaccttct
     3241 ggcgtgccag catcccgggc attgatgcca aacgcagtgc cgaacttaaa gaaaagattc
     3301 agacccagct ggacgccgac gatcagctgc tttcgaccta taaaaaagat tccgcgctga
     3361 tgcgctttaa cgactcgcaa agtttgtcgc cgtggccagt aagtgaagcg atggccgaaa
     3421 tcgtcactac ctcgctgcgc attggcgcga agaccgatgg tgcgatggat attaccgtcg
     3481 ggccgctggt gaatctgtgg ggctttggcc cggaacaaca gccggttcaa attccgagcc
     3541 aggaacagat cgatgcgatg aaagccaaaa ccggcttaca gcacctgacg gtcatcaatc
     3601 agtcgcatca gcaatatctg caaaaagacc tgccggattt atatatcgat ctctccaccg
     3661 tcggtgaagg ttatgcggcg gatcacctgg cacgcttgat ggagcaggaa gggatttccc
     3721 gctatctggt gtcggtgggc ggcgcgctga acagccgtgg tatgaacggt gaaggccagc
     3781 cgtggcgggt agcgattcaa aaaccaaccg ataaagaaaa cgcggttcag gctgtggtgg
     3841 atatcaacgg tcatgggatt agcacctctg gcagctaccg taactattac gaactggacg
     3901 gcaaacgtct ttcccatgtt atcgatccgc aaaccgggcg tcccatcgaa cacaatctgg
     3961 tatccgtgac ggtgattgct ccgacggcgc tggaagccga tgcctgggat actggcttga
     4021 tggtactcgg gccggagaaa gccaaagagg ttgttcgccg ggaagggctg gcggtttata
     4081 tgatcaccaa agaaggcgat agctttaaaa cctggatgtc gccgcagttt aaaagcttcc
     4141 ttatcagcga aaaaaattaa agcgcaagat tgttggtttt tgcgtgatgg tgaccgggca
     4201 gactaaaggc tatccttaac cagggagctg attatgaaaa aagccacatg cttaactgac
     4261 gatcaacgct ggcaatctgt cttagcccgc gacccgaatg ccgacggcga attcgttttc
     4321 gccgtgcgta ccacaggcat cttttgccgt ccgtcttgcc gcgccagaca tgctttgcgg
     4381 gaaaacgtct ccttctacgc aaatgccagc gaggcactcg ccgctggctt tcgcccctgc
     4441 aaacgttgtc agccagacaa agccaatgcc cagcaacatc ggttggataa aattacccac
     4501 gcctgtcgac tgctggaaca ggaaacgcct gtaacgctgg aagccttagc cgaccatgtg
     4561 gcgatgagtc cgttccatct gcatcggttg tttaaagcga ctaccggaat gacgcctaaa
     4621 gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat cgctggcgaa aggggagagc
     4681 gtgacgacgt ctattcttaa cgccggattc cccgacagca gcagttacta ccgcaaagcc
     4741 gacgaaacgc tgggcatgac ggctaaacaa ttccgtcacg gtggcgaaaa tctggcggtg
     4801 cgttacgcgc tggctgattg tgagctgggt cgttgcctgg tggcagaaag cgagcggggg
     4861 atttgcgcga tattgctggg cgatgatgac gcgacactaa tcagcgagtt gcagcagatg
     4921 tttcccgctg ccgacaacgc gcctgccgat ctgatgtttc agcaacatgt gcgtgaagtg
     4981 atcgccagcc tcaatcaacg cgatacgccg ctgacgttac cgctggacat tcgcggcact
     5041 gcttttcagc aacaagtctg gcaggcactg cgcacgatac cttgcggtga aaccgtcagt
     5101 tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg tacgggccgt tgccagcgcc
     5161 tgtgccgcca acaagctggc tatcataata ccctgtcatc gggtggtccg tggtgatggc
     5221 acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc aactgctgcg ccgcgaagct
     5281 gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc tgaaccgtgg caagagccac
     5341 tggcggctgg tgcggtaatt ttacggcgtt ttgcttttaa tgctgcggag caactgatcc
     5401 gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca gatggtcacc cccgggggat
     5461 ataccatgtc ggtggcgatg accaactgtg ggcatctggg ctggacgacc catcggcaag
     5521 gttatctcta ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc atgccacaaa
     5581 gctttcatga tttatgtcaa cgtgcgacta cggcggcggg ctatccagat ttccagccag
     5641 atgcttgtct tatcaaccgc tacgctgctg gcgcgaaact gtcgctgcat caggataaag
     5701 acgaaccgga tctgcgcgcg ccgattgttt ctgtttctct gggcttacct gcgatttttc
     5761 aatttggtgg cctgaaacgt aatgatccgc tcaaacgttt gttgttggaa catggcgatg
     5821 tggtggtgtg gggcggtgag tcgcggctgt tttatcacgg tattcaaccg ttgaaagcgg
     5881 ggtttcatcc actcaccacc gactgccgct acaacctgac attccggcag gcaggtaaaa
     5941 aagaataaaa ataagaatta ttattgctgt gcgcgcgaag atgtttaaac tgcgggctgt
     6001 aattcattgt ccgggttttc tgcatggaac ttcttgtact tgtctggcgg cagtatcgct
     6061 ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc ggcattaggc attggcttaa
     6121 ttgcttttat caatcagcgc cttatcgaaa cggcggatac cagtctgctg gtgttgccgg
     6181 agtttctggg attattgctg ctgttgatgg cagtcactct cggatcgcaa ctggcgctca
     6241 ccactttggg gcatcacttc gtttaccgac tgcgtagtga atttatcaag cggattctgg
     6301 atacccacgt cgagcgcatt gaacaactcg gtagcgcctc gttgctggcg ggattaacca
     6361 gcgatgtgcg caatatcacc attgcttttg tgcgtcttcc ggaactggtg caggggatca
     6421 ttctcactat cggttccgcg gcgtatctgt ggatgctgtc gggcaaaatg ttgctggtaa
     6481 cggctatctg gatggcgatc accatctggg gtggttttgt actggtggcg cgggtgtaca
     6541 aacatatggc gaccctgcgt gaaaccgaag acaggctgta tacggatttt caaactgtac
     6601 tcgaagggcg caaagagctg actctgaacc gggaacgcgc cgagtatgtg tttaacaacc
     6661 tctacattcc tgatgcgcaa gagtatcgcc accatattat ccgcgcagac accttccatc
     6721 ttagcgccgt gaactggtca aacatcatga tgttgggcgc aatcggcctg gtgttctgga
     6781 tggcgaacag tctcggttgg gctgatacca acgttgccgc gacctattcg ttgacgcttt
     6841 tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt gccgacgctg ctgacggcgc
     6901 aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc tttcaaagca gagtttccgc
     6961 gcccgcaggc gtttcccaac tggcaaacgc tggagctgcg taacgtgacg tttgcttatc
     7021 aggataacgc gttttccgtt ggtccgatta atctcaccat caaacgtggc gagctgctgt
     7081 ttctgattgg cggcaacggt agcggaaaat cgacgctggc gatgttgttg acgggcttgt
     7141 atcagccaca aagcggcgaa atcttgctgg atggcaaacc tgtcagcggc gaacaaccgg
     7201 aagattatcg caaactgttt tcggcagtgt ttaccgatgt ctggctgttt gatcaactgc
     7261 tggggccgga gggtaaaccc gctaacccgc aactggttga gaagtggctg gcgcagctga
     7321 aaatggctca taagcttgag ttaagcaacg ggcgtattgt taacctgaag ttatcaaaag
     7381 ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga agaacgcgat attatcctgc
     7441 tggatgaatg ggcggcggat caggatccac acttccgtcg tgagttttat caggtgttgc
     7501 tgccgctgat gcaggagatg ggtaaaacta ttttcgctat cagtcatgat gatcattact
     7561 ttatccacgc cgaccgcctg ctggaaatgc gcaatgggca acttagcgag ctgacgggcg
     7621 aagagcgcga tgccgcttcg cgtgatgccg ttgcccggac ggcataatat tacgacgctg
     7681 acctggcgga ctgctgccgt caggtcaata tgctaaaaat cccttcattt ctaataaccc
     7741 tataattaat caacgaaatt ataatgtttc taaaattaga atataattta taaacattat
     7801 ttaaatgttg ttacttaagt gttaccgttg atgccgcgca aatctcacct gcaataaagc
     7861 gactaaaagt aaggcattaa caagatgaaa aaagtgactg ccatgctctt ctcgatggcc
     7921 gtggggctta atgccgtttc gatggcggca aaagcgaaag cgtccgagga gcaggaaact
     7981 gatgtactgt tgattggcgg cggcattatg agcgccacgt tggggaccta tttacgcgag
     8041 ctggagcctg aatggtcgat gaccatggtg gagcgcctgg agggtgtcgc gcaggagagt
     8101 tcgaacggct ggaataacgc cggaaccggg cattctgcac tgatggaact gaactacacc
     8161 ccgcaaaacg ccgatggcag catcagtatt gaaaaagcag tcgccattaa cgaagcattt
     8221 cagatttccc gccagttctg ggcgcaccag gttgagcgcg gcgtgctgcg tactccgcgt
     8281 tcatttatca ataccgttcc gcatatgagc tttgtctggg gcgaggataa cgttaatttc
     8341 ctgcgcgccc gttacgccgc gttgcaacaa agctcgctgt ttcgcggtat gcgttactct
     8401 gaagatcacg cgcagatcaa agagtgggca ccgttagtga tggaagggcg cgatccacaa
     8461 cagaaagtag cggcaacacg cacagaaatt ggcactgatg tgaactatgg cgagatcacc
     8521 cgccagttaa ttgcttcctt acagaagaaa tctaacttct cgctgcaact cagcagcgaa
     8581 gtccgcgccc taaagcgtaa tgacgataac acctggaccg ttaccgttgc cgatctgaaa
     8641 aacggcactg cacagaacat ccgcgccaaa tttgtcttta tcggcgcggg cggtgcggcg
     8701 ctgaagctat tacaggaatc ggggattccg gaagcgaaag actacgccgg tttcccggtg
     8761 ggcggacagt tccttgtttc ggaaaacccg gacgtggtta atcaccatct ggcgaaggtt
     8821 tacggtaaag catccgttgg cgcaccaccg atgtcggttc cgcatatcga tacccgcgtt
     8881 ctggacggta aacgcgtagt gctgtttggg ccatttgcca ccttctcaac caaattcctc
     8941 aaaaacggtt cattgtggga tctgatgagt tccaccacca cctctaacgt gatgccgatg
     9001 atgcacgtcg ggctggataa tttcgatctg gtgaaatatc tggtgagtca ggtgatgttg
     9061 agtgaagagg atcgttttga agcgttgaaa gagtactatc cgcaggcaaa aaaagaggac
     9121 tggcgtttgt ggcaagcggg gcagcgcgtg cagattatca agcgtgatgc cgagaaaggt
     9181 ggcgtactgc gtctgggtac tgaagtggtc agtgaccagc aagggaccat tgccgcgctg
     9241 ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta tgttggatct gctggaaaaa
     9301 gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta cgttgaaagc gatcgttccg
     9361 tcttatggac gcaagctgaa cggtgatgta gcggcaacag aacgcgagtt gcagtacacc
     9421 agcgaagtgc tggggctgaa atacgacaag ccgcaggcag cagatagtac gccaaaaccg
     9481 cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta atgatgtgcc
     9541 acatccggca tggtatgccg gatgtggcgt atgctgataa gacgcgccag cgtcgcatca
     9601 ggcaccggct cgggcgttag atgtcggatg cggcgtgacc gccttattcg gcctacgggg
     9661 tgccgcactt cacagcattt tagcgaacta ccgcgttgtc aattttctct tccgccttcc
     9721 agacgcggta cttcacatct acattgtctg gcgtatacac cacgatcggc agcttgctgt
     9781 tgtaacgcag cattccagca tcgcccagat acgcggtgac aaatttcttc tctttcttgc
     9841 catccgggca ggccatcatc gtggaaaccg gcgagctaac tttatcaaag acataataat
     9901 catagcccca gccttccagc gttttgcttt ccagcttccc gccgagacga tgcaaattgc
     9961 aatcgacttc cagcgtctga ccgattaaca gttctacttt cagggtagat tcatcttctt
    10021 gcggggttaa ctgaatcacc tgacgcttca tccctttttc agcttgtgga taaggcgcga
    10081 ttttttccag tggctggacg ctttctgccg cccaggcgga agtggtagcg aaagcggcaa
    10141 acaatactgc aggtagaatg gtcttcattt ttttgctttc cttcttcatt tataggtttt
    10201 caccgatgat gtcgctgaag gttaacatat tcatcagatt tattaatttg ctttatgaat
    10261 aacttaaaga acattcagac aattttacct gctcttttag tgttaaattc tgatgaggga
    10321 gcaaaccagc agaaatatct ctggtgaatt aatcgctata taaatatgtt tataaccatt
    10381 tgaaatgtga gcaaaagccc gtttttgcca cgcttcccgc cactcttttg atcctgctac
    10441 aggttttacc ccgatcgggg tatgcatctt tgacacatcc tttaatatct tagcggctat
    10501 aaaaatggct tattaattat gcggtttatt tggtcgctct caattttcag agcgcgttaa
    10561 tgatggaagg tcaatgtgaa gattgatgca tcccgtcggg gcatactcac tggtcgctgg
    10621 cgcaaagcca gtaacggtat ccgtccaccc tggtccggtg atgaatctca ttttctgacc
    10681 cattgcaccc gttgcgacgc ctgtattaat gcttgtgaaa acaacattct gcaacgcggc
    10741 gcgggcggct atccgagcgt taatttcaaa aataacgagt gcagcttctg ttacgcctgc
    10801 gcgcaggctt gccccgaatc gctattttct ccgcgccaca ccagggcctg ggatttgcag
    10861 ttcaccattg gggacgcgtg cctggcgtat cagtcagttg aatgccgccg ctgtcaggat
    10921 agctgtgagc caatggcaat tatctttcgc ccgacgctct ccggaatcta ccagccgcaa
    10981 cttaatagcc aactttgcaa cggatgtgga gcatgtgcag ccagctgtcc ggtatcagcc
    11041 ataactgcgg agtatctcca tgcacactaa ctggcaagtt tgcagcctgg tcgtgcaggc
    11101 caaaagcgaa cgaatttcag acatcagcac ccaactgaac gcctttcccg gctgtgaagt
    11161 tgctgtcagc gacgcgccga gcggtcagtt gattgtggtg gtggaagcag aagacagcga
    11221 aacgctgatc caaaccattg agtcagtacg caacgtagag ggcgtgctgg cggtgtcgct
    11281 ggtttatcac cagcaggaag agcaaggtga ggaaacacca tgaaactcag tcgtcgtagc
    11341 tttatgaaag ctaacgccgt tgcggccgct gcggcggctg ccggtctcag cgtgccgggc
    11401 gttgcccgcg ccgttgttgg tcagcaggaa gccattaaat gggataaagc gccgtgccgt
    11461 ttctgcggta ctggttgcgg cgttctggtc ggaacgcagc aggggcgtgt ggtggcctgt
    11521 cagggcgacc cggacgcacc ggttaaccgt ggcctgaact gcattaaggg ctatttcctg
    11581 cccaagatca tgtacggtaa agaccgtttg acgcagccgc tgctgcgtat gaaaaacggt
    11641 aaatatgaca aagaaggcga atttacccca atcacctggg atcaggcctt cgatgtgatg
    11701 gaagagaaat tcaaaaccgc cctgaaagaa aaaggaccgg aatcgatcgg tatgttcggt
    11761 tctggtcagt ggactatctg ggaaggttat gccgcgtcca agctgttcaa agcgggcttc
    11821 cgttcgaaca acatcgaccc gaacgcacgt cactgtatgg cgtcggcagt agttggcttt
    11881 atgcgtacct ttggtatgga tgagccgatg ggctgctatg acgacatcga gcaggctgac
    11941 gcgtttgtgc tgtggggcgc taacatggcg gagatgcacc cgatcctctg gtcacgcatc
    12001 actaaccgtc gtctctctaa ccagaacgtc accgtggcgg tgctttctac ctaccagcat
    12061 cgtagcttcg agctggcgga taacggcatc atctttacgc cgcaatctga cctggtgatc
    12121 ctgaactaca tcgccaacta tatcattcaa aacaatgcga taaatcagga cttcttcagc
    12181 aagcacgtta acctgcgcaa aggggcgacg gacatcggct acggtttacg tccgacccat
    12241 ccgctggaaa aagcagcgaa gaatccgggt tctgacgcct ccgaaccgat gagctttgaa
    12301 gattacaaag ccttcgttgc tgagtatacg ctggaaaaaa ccgctgaaat gaccggcgtg
    12361 ccgaaagacc agttagaaca actggcgcag ctgtatgccg atccgaacaa gaaagtcatc
    12421 tcctactgga cgatgggctt caaccagcat acccgtggtg tgtgggccaa caacctggtc
    12481 tacaacctgc acctgctgac cggcaaaatt tcccagccgg gttgcggtcc gttctccctg
    12541 accgggcagc cttctgcgtg tggtactgcg cgtgaagtgg gcacctttgc tcaccgtctg
    12601 cctgcggaca tggtggtgac taacgagaaa catcgtgata tctgcgagaa gaagtggaat
    12661 atcccgggcg gcaccattcc ggcaaaaatc ggtctgcacg cggtggcaca agaccgtgcg
    12721 ctgaaagacg gcaagctgaa tgtttactgg accatgtgta ccaacaacat gcaggccggg
    12781 ccgaacatca atgaagagcg tatgccgggc tggcgcgatc cgcgcaactt catcatcgtc
    12841 tccgatccgt atccgacagt cagtgcgctg gccgccgact tgatcctgcc gaccgcaatg
    12901 tgggtagaga aagagggcgc ttacggtaac gccgaacgcc gtactcagtt ctggcgtcag
    12961 caggtacagg cgccaggcga agcgaaatcg gatctctggc agttagtcca gttctcccgc
    13021 cgcttcaaaa ctgaagaagt atggccggaa gagctgctgg cgaagaaacc ggaactgcgt
    13081 ggcaaaacgc tgtacgaagt tctgtatgcc acccccgaag tgagcaaatt cccggtatcc
    13141 gaactggcgg aagatcagct gaacgatgaa tcccgcgagc tgggcttcta tctgcaaaaa
    13201 gggctgttcg aagagtacgc atggtttggt cgcggtcacg gtcacgatct ggcgccgttc
    13261 gatgactacc acaaagcgcg cggtctgcgc tggccggtgg tgaacggtaa agaaacacag
    13321 tggcgttaca gcgaaggtaa cgacccgtac gtgaaagcgg gcgaaggcta taagttctac
    13381 ggtaaaccgg atggcaaagc ggtgatcttc gcactgccgt tcgaaccggc ggcggaagca
    13441 ccggatgaag agtacgacct gtggctctct accggacgcg ttctggagca ctggcacacc
    13501 ggcagtatga ctcgccgtgt gccggaactg caccgcgcct tcccggaagc ggtcttgttt
    13561 attcacccgc tggatgcgaa agcgcgcgat ctgcgccgtg gcgacaaagt gaaagtggtt
    13621 tctcgccgtg gcgaagtgat ctcgattgtt gaaacgcgcg gtcgtaaccg tccgccacag
    13681 ggcctggtgt acatgccgtt cttcgacgcc gcacagctgg ttaacaaact gacgctggat
    13741 gcgaccgatc cgctctcgaa agagacggac ttcaagaagt gcgcagtcaa actggagaag
    13801 gtgtaagcca ttatgtcccg gtcagcgaaa cctcaaaatg gtcgccgccg ctttctgcgc
    13861 gatgtcgttc gcacagcagg cgggctggct gccgtgggtg tggcgctggg gttacaacag
    13921 caaaccgcac gcgcatctgg cgtgcggttg cgcccgcccg gagccataaa cgagaacgcc
    13981 tttgccagtg cctgtgtgcg ttgcggtcag tgtgttcagg cttgccctta cgacacctta
    14041 aaactggcga cgctggcctc tggtctgtcg gcgggcacgc catattttgt cgcacgggat
    14101 attccttgcg aaatgtgtga ggacattccg tgcgccaaag tgtgcccaag cggtgcgctg
    14161 gatcgtgaga ttgaatcgat cgacgacgcg cggatggggc tggcggtact ggtggaccag
    14221 gaaaactgtc tcaactttca ggggctgcgc tgcgatgttt gttatcgcga atgcccgaaa
    14281 attgatgagg ccatcaccct ggagctggag cgcaacacgc gtaccggtaa gcacgcccgc
    14341 tttctgccga cggttcacag cgacgcctgt accggttgcg gtaagtgcga aaaagtatgc
    14401 gtgctggaac aaccggcaat caaggtgtta ccgctgtcac tggcgaaagg ggagttaggt
    14461 caccattacc gcttcggttg gctggagggg aacaatggca aatcgtaaac gtgacgccgg
    14521 gcgcgaggcg ctggagaaaa aaggctggtg gcgcagtcac cgttggctgg tgttacgtcg
    14581 cctttgtcag ttcttcgtgc tggggatgtt tttgagcggt ccatggtttg gtgtgtggat
    14641 cttgcacggc aactacagca gtagcctgtt attcgacacc gttccgctga ccgatccgct
    14701 gatgacgctg caaagtcttg ccagtggtca tctgcctgcc acggtggcgt tgaccggtgc
    14761 ggtgattatc accgtgctct acgccctggc gggaaagcga ttattttgca gctgggtctg
    14821 cccgctgaac ccgattaccg acttagcgaa ctggttacgc aggcgctttg acctcaatca
    14881 gtctgcgacg atccctcgcc acattcgcta cgtgctgttg gtggtcattc tgctgggatc
    14941 ggccttaact ggcacgctca tctgggaatg gatcaacccc gtatctctga tggggcgcag
    15001 cctggtaatg ggcttcggca gcggcgcgct gcttattctc gcgctgtttt tatttgatct
    15061 actggtcgtt gaacacggct ggtgcgggca catttgtccg gttggcgcgc tgtatggcgt
    15121 gctgggtagc aaaggtgtga ttaccgttgc ggccaccgat cggcagaaat gtaaccgctg
    15181 tatggattgt tttcatgttt gcccggaacc gcatgtgcta cgtgccccgg tgctggatga
    15241 acaaagcccg gtgcaggtca ccagccgcga ttgcatgacg tgcggtcgct gcgtggatgt
    15301 ctgttctgag gatgtattta caataactac acgatggagt tcgggagcga aatcatgaaa
    15361 agccatgacc tgaagaaagc gctgtgtcaa tggacggcga tgctggccct ggtggtaagc
    15421 ggcgcggttt gggcggctaa tggcgtcgat tttagccagt cgccggaagt atccgggaca
    15481 caggaagggg ccattcggat gccaaaagag caggaacgga tgccgctgaa ctatgtgaat
    15541 cagccgccaa tgatcccgca tagcgttgaa ggttatcagg taacgaccaa taccaaccgc
    15601 tgcttgcagt gccacggtgt cgaaagttat cgcaccactg gcgcgccgcg tatcagtcct
    15661 actcacttta tggacagcga cggcaaagtg ggcgcggaag tggctccgcg tcgttatttc
    15721 tgtctgcaat gtcacgtacc gcaggccgat accgcgccaa tcgtggggaa tacctttacc
    15781 ccatcaaaag gttacgggaa ataagaggtt attatgggaa attctgaccg taagcctggt
    15841 ctgattaagc gcctgtggaa atggtggcgt acccccagcc gtctggcgct ggggacgctg
    15901 ctgttgatcg gttttgttgg cggcatcgtc ttctggggtg gctttaacac cgggatggaa
    15961 aaagccaata ccgaagagtt ctgcattagc tgccacgaaa tgcgcaacac ggtgtatcag
    16021 gaatacatgg attccgtgca ctacaacaac cgtagcggcg tccgtgcgac ctgtccggat
    16081 tgtcacgttc cgcacgagtt tgtgccgaag atgatacgca agctcaaagc aagtaaagag
    16141 ctgtatggta aaatttttgg cgttattgac acgccgcaga aatttgaagc tcatcgtctg
    16201 acgatggcac agaatgagtg gcggcgcatg aaggacaata actcgcagga gtgccgtaac
    16261 tgtcacaact tcgagtatat ggatacaacc gcccagaaat cggttgccgc gaagatgcat
    16321 gaccaggcgg tgaaagatgg gcaaacctgt attgattgcc ataaagggat agcgcacaag
    16381 ctgcccgata tgcgtgaagt cgagccaggt ttttaacagg gttattgcgt gggtatgctt
    16441 gaagccagag agttactttg tgagcgggat gaacgaacct tatttagtgg cttgtcattt
    16501 acgctgaacg caggagagtg ggtacaaatc accggtagca acggcgcggg gaagacaacg
    16561 cttctccgtt tgctgacggg gttgtctcgc cctgacgcag gcgaggttct ctggcaaggg
    16621 cagcccttgc atcaggtacg cgacagctac catcaaaacc tgttatggat aggccatcag
    16681 ccggggatca aaacccggct gacggcgtta gaaaatctgc acttttatca tcgcgatggc
    16741 gataccgcac aatgtctgga agccctggcg caggccgggc ttgccggatt cgaagatatt
    16801 cctgtaaatc agctctcggc cgggcaacaa cgccgcgtcg ctttagcgcg tctgtggctg
    16861 acccgtgcca cgttatggat cctcgacgag ccttttaccg cgattgacgt taacggcgtc
    16921 gatcgtctga cccagcgtat ggcgcagcat acggagcagg gggggattgt gattctgact
    16981 acccaccagc cgctcaacgt tgctgaaagt aaaattcgcc gcatttcact gacgcaaacg
    17041 agggccgcat gatgttctgg cgcattttcc gtcttgagct gcgtgtagcg tttcgccata
    17101 gcgccgaaat cgccaacccg ctgtggttct tcctgattgt aattaccctt tttccgctca
    17161 gtatcggtcc ggagccgcaa ctgctggcgc gtattgcacc gggcattatc tgggttgctg
    17221 cgctgctttc atccttgctg gcgctggaac gactgttccg tgacgatttg caggacggca
    17281 gtcttgaaca attgatgttg ttgccgttac ccttgcccgc cgttgtgctg gcgaaggtga
    17341 tggcgcactg gatggtaacc ggtctgccgt tactcatcct ttcgcccctg gtagcaatgc
    17401 tactgggaat ggatgtttat ggctggcaag tgatggcgct gacgctgctg ctgggaacgc
    17461 ctacgcttgg ctttctcggt gcaccgggcg tggcgctgac agtgggactt aagcgcggtg
    17521 gggtgctgct cagcatactg gtgttaccgc tgactatccc attactcatc tttgccaccg
    17581 ccgcgatgga cgcggcttcc atgcatttgc ccgttgacgg gtatctggca attttaggcg
    17641 cgttgctggc aggcaccgcg acattaagtc cttttgcgac ggcggcagcg ttacgaatca
    17701 gcattcaata acgctggatt actgattttt gttcgagtct ggtatcgaaa ctatgtggaa
    17761 aacactgcat caactggcga tcccaccacg gctgtatcaa atctgtggct ggtttatacc
    17821 gtggctggca attgccagtg tggtcgtgct taccgtcggc tggatctggg gattcggctt
    17881 tgctccggct gattatcagc agggaaatag ctaccgcatt atctacctgc atgtgcctgc
    17941 ggcgatctgg tcgatgggca tttatgcatc aatggcagtg gcagcgttta ttggccttgt
    18001 ctggcagatg aaaatggcca acctggcggt ggcggcgatg gcccccattg gtgcagtgtt
    18061 tacctttatt gccctggtta ccggctctgc atggggaaaa ccgatgtggg gcacctggtg
    18121 ggtatgggat gcacgtctga cttctgaact ggtgctgctg tttttgtatg tgggtgtgat
    18181 cgccctgtgg cacgccttcg acgaccgccg tctggcgggc cgtgcggcag gtatcctggt
    18241 gctgattggc gtggtgaatc tgccgattat tcattactcc gtggagtggt ggaacaccct
    18301 gcatcaggga tcaacgcgaa tgcagcaaag tatcgatccg gcgatgcgtt cgccgctgcg
    18361 ctggtcgatt tttggcttcc tgctgctgtc tgccacgctg acgctgatgc ggatgcgtaa
    18421 tttgattttg ctgatggaaa aacgccgtcc gtgggtgagt gaactgatac tgaaaagagg
    18481 ccgtaaatga cccctgcatt tgcttcctgg aatgaatttt tcgcaatggg cggttacgcc
    18541 ttttttgtct ggctggcggt ggtgatgacc gttattccgc tggtggtttt ggtcgtgcac
    18601 tcggtgatgc agcatcgcgc aattctgcgt ggtgtggcgc aacagcgggc gcgtgaggcg
    18661 cgtttacgtg ctgcgcaaca gcaggaggct gcatgaatat tcgccgtaaa aaccgcttgt
    18721 ggatcgcctg tgccgtgttg gcagggctgg cgctgactat cggtctggtg ctatatgcgc
    18781 tgcgctcgaa tatcgatctc ttttatacgc cgggggagat tctctacggc aagcgtgaga
    18841 ctcagcagat gccggaagtc ggtcagcgtc tgcgcgttgg cgggatggtg atgccgggga
    18901 gtgtgcagcg cgatcccaat tcgctaaaag taactttcac catttacgac gctgaaggtt
    18961 cagtagatgt ctcttacgaa ggcattttgc cggatctgtt ccgtgaaggg cagggcgttg
    19021 tggtgcaggg cgaactggaa aaaggcaatc atatcctcgc aaaagaagtg ctggcgaaac
    19081 acgacgaaaa ctacacgccg ccagaagttg agaaagcgat ggaagccaat caccgtcgcc
    19141 cggcgagtgt ttataaggac ccagcatcat gatgccggaa attggtaacg ggctgctgtg
    19201 tctggcgcta ggaattgcgc tgctgctgtc cgtgtatccg ctatggggcg tagcgcgcgg
    19261 agatgcgcgc atgatggcgt cttcccgctt gtttgcctgg ctgctgttta tgtctgtggc
    19321 tggcgcattt ctggtactgg tcaatgcctt cgtggtcaac gacttcaccg tcacctatgt
    19381 tgccagcaac tccaataccc agcttccggt gtggtatcgc gtggcggcta cctggggcgc
    19441 gcatgaaggc tcgctcctgc tgtgggtgct gctgatgagc ggctggacct ttgcggtagc
    19501 gatttttagt cagcgtattc cgctggatat tgtggcccgc gtactggcga taatggggat
    19561 ggtcagcgtc ggctttttgc tgttcattct ctttacctct aacccgttct cacgcacgtt
    19621 gccgaacttc ccgattgaag ggcgcgatct taacccgctg ttacaggatc cggggctgat
    19681 cttccatccg cctctgctct atatggggta cgtggggttc tcggtggcgt ttgcttttgc
    19741 cattgcttct ttgttgagcg ggcgtctgga cagcacttat gcgcgtttta ctcgtccgtg
    19801 gacgctggcg gcgtggatct tcttgacgct cggcattgtg ctcggttccg catgggccta
    19861 ttacgaactc ggctggggcg gctggtggtt ctgggatccg gtagaaaacg cctcgtttat
    19921 gccgtggctg gtggggactg cgctgatgca ctcactggcg gtcactgaac aacgcgccag
    19981 cttcaaagcg tggacattac tgctggcaat cagtgccttc tcgttgtgtc tgctggggac
    20041 tttcctggtg cgttccggcg tgctggtatc ggtacacgcg tttgcctctg atccggcacg
    20101 cggtatgttt atcctcgcct ttatggtact ggtgattggt ggttcgctgc tgctgtttgc
    20161 cgcgcgtgga cacaaagttc gctcacgcgt aaacaatgcg ctgtggtcgc gggaatctct
    20221 gctgttagcg aacaacgttt tgctggtcgc cgcgatgctg gtggtattac tggggacgct
    20281 gctgccgctg gtgcacaagc aactgggact gggcagtatt tcgattggcg aaccgttctt
    20341 caacaccatg tttacctggc tgatggtgcc gtttgcgctg ctgcttggtg tcggtcctct
    20401 ggtgcgctgg gggcgcgatc gcccacgtaa aatccgcaat ttattgatta tcgccttcat
    20461 ctcaacgctg gtgctgtcgc tgctgctgcc gtggctgttc gaaagcaaag ttgtggcgat
    20521 gacggtgctc ggcctggcaa tggcctgctg gattgctgtg ctggcaattg cggaagctgc
    20581 gctgcgtatt tcacgtggca cgaaaaccac cttcagttat tggggaatgg tggcggctca
    20641 cctggggctg gcagtgacaa ttgttggcat tgcttttagc cagaactata gcgttgagcg
    20701 tgatgtgcgc atgaagtccg gcgatagcgt cgatattcat gaatatcgct tcaccttccg
    20761 tgatgtcaaa gaggtgactg gcccgaactg gcgtggcggt gtggcgacta tcggcgtaac
    20821 gcgcgatggc aagccggaaa cggtgctgta tgcggaaaaa cgttattaca acactgccgg
    20881 gtcgatgatg accgaagcgg caattgacgg cggcatcacg cgtgacctgt acgccgcgct
    20941 cggtgaagag ctggaaaacg gcgcgtgggc cgtgcgtctt tactacaaac catttgttcg
    21001 ctggatttgg gcgggcgggc tgatgatggc gttgggcgga ctactgtgtc tgtttgatcc
    21061 tcgctatcgt aagcgcgtga gtccgcaaaa aactgcgccg gaggccgtat gaagcgcaaa
    21121 gtattgttaa ttccgttgat tatcttcctg gcgattgccg cggcgctgct gtggcagctg
    21181 gcgcgtaatg ccgaagggga tgatccgacc aatctggaat cggcgctcat tggcaagcct
    21241 gtgccgaagt ttcgtctcga atcactggac aatccggggc agttttatca ggcggatgtg
    21301 ctgactcagg gcaaaccagt gctgcttaac gtctgggcga cctggtgtcc gacctgccgt
    21361 gcggaacatc aatatctgaa tcagctttca gcgcagggca ttcgcgtggt cggcatgaac
    21421 tataaagacg atcgccagaa ggcaatcagc tggctgaaag agctgggcaa tccttacgcg
    21481 ctaagcctgt ttgatggcga cggcatgtta gggctggatc tcggtgtcta tggcgcgcca
    21541 gaaaccttcc ttattgacgg caacggtatc attcgctatc gccatgcggg cgatctcaat
    21601 cctcgcgtct gggaagaaga gatcaagccg ctgtgggaga aatacagtaa ggaggccgca
    21661 caatgaggtt tttattgggc gtgctgatgc tgatgatctc cggctcagca ctggcgacca
    21721 tcgacgtgtt gcagtttaaa gatgaagcac aggaacaaca gttccgtcag ctcactgaag
    21781 aactgcgctg cccgaaatgc cagaacaaca gcattgccga ttccaactcg atgattgcca
    21841 ccgacctgcg tcagaaagtg tatgaactga tgcaggaagg taaaagtaaa aaagagattg
    21901 tcgattatat ggtggcgcgt tacggcaact tcgtcactta cgatccgccg ttaacgccgc
    21961 tgaccgtgct gctgtgggtg cttccggtag tggctattgg cattggcggt tgggtcatat
    22021 acgcccgctc gcggcgtcgg gtacgcgtag tgccggaagc gtttcctgaa caaagcgtgc
    22081 aggaaggtaa gcgtgccgga tatattgttt atttgccggg cattgtggtg gcgttaattg
    22141 tggctggcgt cagctactac cagaccggaa attatcagca ggtgaaaatc tggcagcagg
    22201 ccacggcaca ggctccggcg ttgctggaca gggcgctgga tccgaaagcc gatccgctca
    22261 acgaagaaga gatgtcgcgt cttgcgctgg ggatgcgtac tcaactgcaa aaaaatccgg
    22321 gagatataga aggctggatt atgttgggcc gcgttggcat ggcgctgggt aacgctagta
    22381 tcgccaccga tgcatacgct actgcgtatc gcctcgatcc gaaaaacagc gatgccgcac
    22441 tgggttatgc tgaagcgttg acacgttcat ctgatcccaa cgacaaccgc ctcggtggtg
    22501 aactgctgcg ccagttggtg agaagcgacc acagcaatat ccgtgtgtta agcatgtatg
    22561 cgtttaatgc ctttgagcag cagcgatttg gcgaagccgt tgccgcgtgg gagatgatgt
    22621 tgaaactctt acctgccaac gatactcgcc gtgcggtgat tgaacgtagt atcgcgcagg
    22681 cgatgcaaca tttgtcgccg caggagagta aataaaaaga ggcccgatgg tgatgccatc
    22741 gggctatttt tattgtgccc cgcgttgttg caggaacaga atggtggctg ccacgcgtga
    22801 gcggacattg agtttgcgca gcagattgcg aatatgtact tttactgtct gctctgaaat
    22861 attcaacacc gaggcaatct gtttatttga cagcccctgt gccagctcgt gcagaacatc
    22921 cagctcgcgc tccgtcagca cgctgaaggg atcttcttcc gcgccaaaca tttcacgttc
    22981 acgtaagtac tgattgacgc gttcgctaaa gactttgctg cctttcgctc cagcacgaat
    23041 tgcttccagc aatacttccg ggtcgctgtc tttcaacaga taaccgtctg cgcctgcgtc
    23101 tatcagtgca aagacatcgc tggaggcatc tgatacggtc aggataataa tttgcgcggt
    23161 aacgccatcc ctgcgcaagg cattgagggt atccaggcca ctcatacctt tcatattgag
    23221 atccagcaag atcacgtcga tatccagtct attcgccaga tcgatcgcgc tcgcgccgtc
    23281 gcccgcttcg gcgaccactt caaagccagg atcaagctcc agtaactgac gaacaccgcg
    23341 tcgcataagt ggatgatcat ccacaatcat cacctgaaaa ggcgttgctt caggcatagt
    23401 agtctcctga ggttttatta gaatcattat gtttgagcgt agcgttggta acgctaccag
    23461 gcgcgtgaat gttaccccat tttatgtatg gacatgtagt gaaaactaca accttgtcgt
    23521 tcgctttggc atcattttca ttataaagac ggaattttgc atcagcttct catcaagaaa
    23581 aacaactaaa aaatcaatca caatacttgt gcatttcttt aaacagtttt taaaaactga
    23641 ctccgggtat ggagctatgg gtattttccg tacccaatgc ttttaacagc aattaatttc
    23701 ataggatgaa agctcaattc atcaatctgg ttctgtttct ctttgtcgtt ccgcaatatc
    23761 tgttctggtg gctacagcgt tatattcacc catagcattg gcatcaacag ttgagtatgg
    23821 tgagacagtt gatggtgttg tcctggaaaa agatatccag ctggtttatg ggaccgccaa
    23881 taatacgaaa atcaatcctg gcggagaaca gcatataaaa gaatttggtg taagtagtaa
    23941 tactgaaatt aacggagggt atcagtacat tgaaatgaat ggtaccgcag aatactcagt
    24001 attaaatgac ggttatcaaa ttgttcaaat gggtggcgcg gcaaaccaga ctaccctcaa
    24061 taatggtgtg ctacaggttt atggcgcagc gaatgatccc acgattaaag gcgggcgctt
    24121 aatcgttgaa aaagatggga ttaccgtcct tgccgctatc gaaaagggag gattactgga
    24181 ggttaaagag gggggattag cgattgcggt agatcagaaa gcaggcggtg ctattaaagc
    24241 aagcacgcgg gtcatggagg cattcggaac aaaccgtctc ggtcagttcg aaatcaagaa
    24301 tggtattgtt aacaatatgc tgttggaaaa cggcggaagt ttgcgagttg aagaaaatga
    24361 cttcgcttat aatactactg tagatagtgg cggcttactg gaggttatgg atggcgggac
    24421 tgtaactggc gttgataaaa aagcaggcgg aaaattaatt gtctcaacga atgcgctgga
    24481 agtgagtggt acaaacagta aaggccaatt tagtataaaa gatggtgtgt caaaaaatta
    24541 tgaactggat gatggttccg ggctcattgt tatggaggac acgcaggcca ttgatactat
    24601 ccttgatgag catgccacta tgcaatcgct gggaaaggat actggtacga aagtgcaggc
    24661 aaatgcggta tatgatctcg gtcgatcaga tcagaatgga agtatcacgt attcctcaaa
    24721 agccatttct gaaaatatgg ttatcaacaa tggccgcgct aacgtctggg ctggcacaat
    24781 ggttaacgtt tcagtcagag ggaatgatgg cattcttgag gtcatgaagc cgcaaataaa
    24841 ttatgcaccc gcaatgttgg tgggtaaggt agtggtttct gagggcgctt cttttagaac
    24901 gcatggtgcc gtggatacca gcaaagcgga cgtttcgctc gaaaatagcg tatggaccat
    24961 cattgccgat atcactacga cgaaccaaaa caccctcctc aacttagcca accttgcgat
    25021 gtctgacgca aatgtgatta tgatggatga gccagtgact cgttcatcag tgacggcaag
    25081 tgcggaaaat ttcattacgt tgaccaccaa taccctgtcg ggaaacggca atttttatat
    25141 gcgtaccgat atggctaatc atcagagcga tcagctcaac gtcaccggtc aggcaacagg
    25201 tgatttcaaa atattcgtga cggacaccgg tgccagcccg gcagcaggag atagccttac
    25261 actggtaaca acgggcggcg gtgatgctgc atttacgttg ggcaatgccg gaggcgttgt
    25321 tgatatcggt acgtatgaat ataccttgct ggataatggc aaccatagct ggagtttggc
    25381 agagaatcgc gcgcaaatta ccccttcaac cactgatgtg ctgaatatgg cggcagcaca
    25441 accgctggta tttgatgcag aactggacac cgtgcgtgag cgtcttggta gcgtaaaagg
    25501 cgttaattac gatacggcga tgtggagttc ggcaattaac acccgcaaca acgtgaccac
    25561 tgatgcgggc gctggctttg agcaaacatt gaccgggctg acgctcggta tcgatagccg
    25621 tttctcccgt gaagaaacca gcacaattcg cggcttgttc tttggttact ctcattctga
    25681 tattggtttt gatcgcggcg gcaaaggcaa tgtcgatagc tataccctgg gggcttatgc
    25741 cggttgggag catcagaacg gtgcctatgt tgatggagtg gtgaaagttg accgttttgc
    25801 caacaccatc cattgcaaga tgagtaatgg ggcaacagcg tttggcgatt acaatagtaa
    25861 cggcgcgggt gctcatgtcg agagcgggtt ccgttgggtt gacggattgt ggagtgttag
    25921 accctatctg gcctttaccg gctttaccac agatggtcag gactacacgt tatcaaacgg
    25981 catgcgcgcg gatgtgggaa atacccggat attacgcgct gaagcgggaa cggcggtaag
    26041 ctatcacatg gacctgcaaa acggtacgac gctggaaccc tgactgaaag ccgccgtgcg
    26101 tcaggaatac gccgattcta accaggtgaa agttaatgac gatggcaaat ttaataatga
    26161 tgtggctgga acccgtggcg tttatcaggc tgggataagg tcatcgttta ccccgacgtt
    26221 aagcggtcat ttgtcagtca gctatggcaa tggcgcaggg gtagaatcgc cgtggaatac
    26281 tcaggcgggt gtggtctgga cgttctgata acaaaaaata aacaggctgt gatgtgtcac
    26341 ggtctgttta tcgaattaat tgcagatata aaaaaaacca accgtaaagg ttgatttttt
    26401 cttgggattt ttggtcggca cgagaggatt tgaacctccg acccccgaca ccccatgacg
    26461 gtgcgctacc aggctgcgct acgtgccgac tcgtggctgc taatactacc gttttccaca
    26521 ccgattgcaa gtaagatatt ttgctaactg atttataatt aatcagttag cgataaaacg
    26581 cttctcgtct gtcagcactt gcaataacaa acttaactgt ggtttttcat ctttcactct
    26641 ttcaccacgt aagttgtaag tgcggtattt accgttattg ttcagcacca gcgtcttttt
    26701 cggggtggta attgccagcg tatcgttatc cgctgcagta acccagtaat gacggcgttg
    26761 agggttgaac aaatcctgac cttgcgaata ttcgctggca ggtgtgctga catgtagcag
    26821 gcgttgcatc agcgtcgtca tcagatcggt atgatcagtc agagcattaa tacgctgcgc
    26881 cggcgtgcct ggccagtgaa tcactaatgg cacctgcaga tgaccgtggg accagtcaaa
    26941 ggtttcttcg tcttcgctca gcggaatacc ccgaccggca gtgataatca ccaccgtatt
    27001 gtccagtttg ccagaatcac gcagtgcatt gagcacgcgg ttgatctggt catcgacatt
    27061 gcctgccgcc cggctatatt tccgtgcaaa tgcctgctga ttgctgtcgt caatgttagt
    27121 gccattgaaa gagacccacg agaaccagcg gttatcttct tgtgcgtagc ggcccagcca
    27181 gttgatccac tgcgtggcgg tctgctcgtc ggattgggtg cgtacgctcg gcatcgagaa
    27241 atctgacaac aatgcctggc gatacagcgg gctggtaaag ccatctgatg agaataatcc
    27301 cagctgatag ccttgctgat taagcgcagt aattaacgcc gcaggggtac gggtcgacag
    27361 aatgccgtcc atatagctcg gcgagatgcc atagaacagg ccaaagatgc cgttgtctgt
    27421 agtgttgccg gagctcatat ggcgcgtgaa cgaaatattt tgctcagcaa aacctgccag
    27481 cgcaggcatc tgcttctcga agcgtgagta gttcaggcca tcgacagtaa tcaacagcac
    27541 attctgcccg gtgcccatat cgcgatagcg cagttcgctt aacggatact gaacggaaac
    27601 ggcgtctgga ttaccttgct caataagacg gcgttgatac tcctgcgcat caagcagacc
    27661 atgcttctca agaaaacgtc gcgccgtcat cgggtacgaa agcggcaggt tagcgcgctg
    27721 catggtgatc gggcgataga agttggcatc ggcccagata tacaccacat gcgaggcgat
    27781 aaaggcgata aataagaatg cggccagcgg gcgcgcgaag cgccgacgac gcgtcaggct
    27841 gcgcagcttt tgccagctcc acgtcgcaaa caccagttca agcaataaaa taaccggcac
    27901 gctgatgaac atcagctgcc agtcgcgcgc catctcattt tcgtctgggt tgataaccag
    27961 ttgccagacg atgggattaa gatggagatg gaaacgagta aagacttcgc tatcgatcag
    28021 taatagcgtc attcccgccg ttgccagaat gacggacaaa aacctcatca gcctctggga
    28081 gccgacgata aaggtcagcg ggaagaggat cagcaagtag gtggcgaaca ccaggaagct
    28141 gaaatggccg ataatgctta cgtaggaata aatgcgacca gcaagcgttg tcggccagtc
    28201 ggcgataaac aggtaacggc tgccaatgac gagcgaaagc agaatattga acagtgcaaa
    28261 ccagtgcccc caactgacca tctgggagac tttttcacgg tagcgctgac gatgagttac
    28321 cataacctgt tatctgtttc ccttagtgcg ctttgtcttc gttgatagag gactgtaagg
    28381 cgcgggcaaa agagttggca attgcctggc gttgggccgg ggcaatgctg gtattgataa
    28441 ggttagtgac catgttgcct aacaccatca gggaaaggtc ggtcggagcc ttatgttttt
    28501 ccagtacgtt gagcagctct gcgagcaatt gttcaacctg ttcatcactg tagcgggaaa
    28561 tttgtggcat aaatcgaaat cagttagttg atgaaagggc aacatattac cgtagcaacg
    28621 gttttttttc tgctttttta tgcgttaaat cattctcgtt acgctgcctt ctgtaaaatg
    28681 accgccatct tttattgcgt cgcttaaccg gcggtggttg aataccgccc ggtcttaaag
    28741 gagagtttat catgagtctg gatatcaacc agattgctct gcaccagctt atcaagcgtg
    28801 atgagcagaa ccttgagctg gtgttgcgcg attcattgct ggaaccgaca gaaaccgtcg
    28861 ttgagatggt ggcagaactg catcgggtct atagcgccaa aaataaagct tacggcttgt
    28921 ttagcgaaga gagtgaactg gcgcagacgc tgcgcttaca gcgtcagggc gaagaggact
    28981 ttctggcatt tagccgcgcg gcaaccgggc gcctgcgtga cgaattagcg aaatatcctt
    29041 ttgctgacgg tggattcgtg ctgttttgcc actaccgtta tctggcggtg gagtatctgc
    29101 tggtggcagt tctgagcaac ctgagcagta tgcgcgttaa cgaaaatctc gacatcaatc
    29161 caacccatta tcttgatatc aatcatgcgg atattgttgc gcgtatcgat ttaaccgaat
    29221 gggaaaccaa tccagagtcc acccgctatc tcactttcct gaaagggcga gtagggcgca
    29281 aagtggcgga tttctttatg gatttcctcg gggccagcga agggttaaac gcgaaagcgc
    29341 aaaaccgtgg gctgttgcaa gcggttgatg atttcactgc cgaagcacaa ctggataaag
    29401 cagagcgaca gaacgtgcgc cagcaggttt atagctactg taatgagcag ctgcaggcag
    29461 gcgaagagat cgaactggag tcgctgtcga aagagctggc aggcgtcagc gaagtgagtt
    29521 tcacggagtt tgcggctgaa aaaggctacg aactggaaga gagcttcccg gcagaccgta
    29581 gcacactgcg tcagttgacg aagtttgccg gtagcggcgg tggcttgact attaactttg
    29641 atgcgatgct gttaggtgaa cgcatcttct gggatccggc aaccgatacc ctgaccatta
    29701 aaggcacgcc gccgaatttg cgtgaccaat tgcagcgccg gacgtctggc ggcaattaat
    29761 cggttttgtg ttctgaatgc cagatgcggc gtgaacgcct tatctggcct gcaaaaaata
    29821 gcaataccgt aatcaggcat aatctgcaga tacaaaaaac cccgccggag cggggttttt
    29881 tacaacttat tcagcaatta agcgcgaacg aagtcgatgt gctgcagctt cggtttgtac
    29941 gggtgacgct gtacgtcctg agctttaact ttgatttctt taccgtcaac aacgatggtc
    30001 agaacttcgc tgtagaattc agctttagct tgcatgttca tgactttgtc gtgatccagc
    30061 tcgatagcca gcggcgcttc tttgccaccg tagatgattg ccgggaactt gttagcggca
    30121 cgcaggcggc ggctcgcacc cttaccctgc tctttacgta cttctgcgtt gatagtaaac
    30181 atttctttct ctcttaaatt aatcctgcta caggcgaccc agcaacaggt aagtgatctg
    30241 ctttgcgtat gcaaaagcgg gcgagattct atactcaatc gctttataca tcaatcaaaa
    30301 ctaccattaa ccgcgtaatt cgtgcgcccg acgaaaacga ccttcgtaat cgaacacttt
    30361 ttcacgcact tgccagtact gacctttcat gcgggcaacg acaaaatccg ggtgtcgcaa
    30421 taaggcttgc tgggcgagga tatcggcggc ggtgatccag cgcagaggga tgcccggtgt
    30481 gcgcgtatgc gggcggataa aaagctgctc gaaggcagtt cgctgggcgg gcgtttgcag
    30541 acggaaacgc tcactcacat ccgcgccgtc ttcatcgtaa taggtgattt tcaaccattc
    30601 gcctttctcg tcgtgctcat gctgcaaaga catgccgcta cagcgtaata ccagcgcatc
    30661 tttcagtcgt agcgccgctt ttaacatatc atccggatca accagtaccg tgtcacattc
    30721 gcggcagcgg cgagcggcaa tatcattttc tgcgttacat tgcgggcaat ttttaaaacg
    30781 gaaacggaag tcacactgtt cgcgatgacc gtcgtcatct tcaaaccatc cctgacagcg
    30841 gcgaccaaag tgttcaatca atgtcccgtc ggcggtcgtt ttcccccaaa aggtgttggc
    30901 aaaaccgcag gcagggcaga aaacctgtac cggaacgttg tcacttttgc ctttcggtgt
    30961 accaacttcc ggcgcgtaga gatcgtgagg attacccgca taatcaagaa ttaaacaatc
    31021 agtcttgccc ggagcgagac gcagaccgcg cccgacaatt tgttggtaaa gactcactga
    31081 ttcggtaggg cgcagaatgg cgataagatc gacgtgcggg gcgtcaaatc cggtggtcag
    31141 tactgcgacg ttgaccagat agcgaaaacg ctgggcttta aaatcttcaa ttaacacatc
    31201 gcgctcagcg ccgggggtgt cgccagtaat cagtgcagca tcttcggcag gcagtaatcc
    31261 cacaatctct tttgcgtgtt caacggtggc ggcgaaaatc attacccctt tgcgcttttc
    31321 ggcgaactcc atgatctggc tgatgatatg tggcgtaatg cgttgctgtt ttttcagctc
    31381 acggttgaga tcagcttcgc tgaacagccc gttactctgt gcctgcaagc ggctgaaatc
    31441 gtattgcact actggcatat ccagtcgttc tggcggcgtc agatagccgt gtttaatcat
    31501 ataacgcagc ggcagctcat aaatgcagtc acggaaaagg gctttctcat cgccgcgtac
    31561 catgccgtga taatggaact ggtagatcca gcctttgccc aatcgaaaag gcgtggcagt
    31621 cagccccagc aggcgtaagt ggggattcac tttagtcagg tgagtgagga tttgctgata
    31681 ctggctctct tcatcgtcac caatacggtg gcattcatcg acaatcaaca gcgaaaattc
    31741 accctgaaag gcatcaagat tacgagcgac cgactgcacg ctgccaaata ccactttacc
    31801 gtggctctct ttgcgcttta acccagcggc aaaaatatcg gcttccagcc ccagcgcctg
    31861 atactttgca tggttttgcg ccaccagttc tttgacgtgt gccagcacca gaacacgacc
    31921 acgcgccagc cgtgccagtt cggcaatcac caggctttta cctgcgccgg tgggcagcac
    31981 gataacggca ggggttttat gacgacgaaa atggttgagc gtggcatcca cggcttcttg
    32041 ctgatatggg cggagtgtaa aaatcatggt ctcgcgtagg tagattattc tgctaatagt
    32101 atgccatgga tctttttccc ttgagggatg ttattagacc gctatactgg gcagacaaca
    32161 gttcagattt tcgggtcaat gcccgacctc cgtttttaca ggcaaaatca cacacatgcg
    32221 acttgataaa tttatcgcac agcaactcgg cgttagccgt gctattgccg ggcgtgaaat
    32281 ccgcggcaat cgtgtcaccg tcgatggcga aattgttcgt aatgcagcgt tcaaactgct
    32341 tcctgaacat gatgtcgctt acgatggcaa cccgctggcg cagcaacatg gtccacgtta
    32401 cttcatgctc aataagcctc agggctatgt ttgctccacg gacgaccctg atcacccaac
    32461 ggtgctctat tttctggatg aaccggtagc ctggaaactg cacgccgcag gacgtctgga
    32521 tatcgatacc accggtctgg tgctgatgac tgatgatggt cagtggtcgc accgcattac
    32581 ttctccgcgc catcattgcg agaagaccta tctggtgacg ctggaatcac ccgtagctga
    32641 cgatacggca gagcaatttg ccaaaggcgt acagctgcat aacgaaaaag atctcactaa
    32701 gccagcggtg ctggaagtga ttacccctac gcaggtccgt ctgaccatca gcgaagggcg
    32761 ttatcatcag gtgaaacgca tgttcgccgc tgtgggtaac catgtggttg agctgcatcg
    32821 tgaacgtatt ggcggtatta cgctggatgc tgatttagcc cccggtgaat atcgtccgtt
    32881 aactgaagaa gaaattgcca gcgtcgtcta acttttccat gactttcagg agcccgttgt
    32941 gaccacccga cagcattcgt cgtttgctat tgtttttatc cttggcctgc tggccatgtt
    33001 gatgccgctg tcgattgata tgtatctgcc cgcgctaccg gtaatttcag cgcagtttgg
    33061 cgtacctgcg ggcagtacgc agatgaccct cagtacttat attctgggct ttgcgttggg
    33121 gcagttaatc tacgggccga tggcagacag ctttgggcgt aagccggtgg ttcttggcgg
    33181 tacgctggtg tttgccgccg ccgcggtggc gtgtgcgttg gcacaaacca tcgatcagct
    33241 gattgtgatg cgtttcttcc acgggctggc ggcggctgcg gccagcgtgg ttatcaacgc
    33301 tttgatgcgt gatatctacc cgaaagaaga gttctcgcgg atgatgtcgt ttgtcatgct
    33361 ggtgacgacc attgcaccgc tgatggcacc gatagttggc ggctgggtgc tggtgtggct
    33421 gagctggcat tacatcttct ggatcctcgc cgtggcggcg attctggctt cggcaatgat
    33481 cttctttctg atcaaagaaa ccttaccacc ggagcgtcgc cagccttttc acattcgtac
    33541 cactattggt aactttgcgg cgctgttccg tcataagcgt gtcctgagtt atatgcttgc
    33601 cagtggtttc agctttgccg ggatgttctc gttcttaagc gccggaccgt ttgtctacat
    33661 tgaaattaac cacgtcgcgc cggaaaactt tggttattac tttgcactaa acattgtttt
    33721 cctgttcgtg atgaccattt ttaacagccg cttcgtccgc cgtattggcg cgttaaatat
    33781 gttccgctcg gggttgtgga tacagtttat tatggcggcg tggatggtca tcagtgcgct
    33841 gctggggctg ggattctggt cgctggtggt tggcgttgcg gcgtttgtgg gctgcgtgtc
    33901 gatggtgtca tccaatgcga tggcggtcat tcttgatgag tttccccata tggcgggaac
    33961 ggcatcttcg ctggcaggaa ccttccgttt tggcataggg gcaattgttg gcgcattgct
    34021 ttctcttgcg acctttaact ctgcatggcc gatgatttgg tcaattgcat tctgcgcaag
    34081 cagctccatt ctcttctgtc tgtacgccag tcgaccgaaa aaacggtgat ctattgcaca
    34141 acgaggaagc taaaaggctt cctttgttga tgcatgtcaa ccacaaatct atcattcccc
    34201 cgatatatgt ttattttatg taaaatcaat ttatgtaaaa agtcacatca ttgtagttaa
    34261 aaaggttgag ttagatcgca gaaacgggta catatagccc cgcaaacgtg accacgcccg
    34321 cagatattac ttaaatcaga accatagagg ccacgcaggc gaggcatcaa tctttacgat
    34381 ctgtataaag acggattgtt gatgatgtgt taaaattgat gtaaacaaat tgtgaagtga
    34441 atgtgcttcc ggggaaaata agtgacttca ttacaactct caatcgtcca tcgactgccg
    34501 cagaactatc gctggtctgc tggtttcgca ggttcgaagg ttgaaccgat tccgcaaaat
    34561 ggaccgtgcg gtgacaacag cctggtggcg cttaaattgc ttagcccgga tggtgataat
    34621 gcatggtcgg tgatgtataa actaagccag gcgttaagcg acatcgaagt tccatgttcg
    34681 gtgctggagt gtgaaggtga gccgtgcctg tttgtaaatc gccaggacga gtttgctgca
    34741 acatgccgat tgaaaaattt tggtgtggca attgccgaac ccttttcaaa ctacaatcct
    34801 ttttaagcgt cagctcaacg ccagtagctg acgcgtatac tcctgctgcg gtgtggcaaa
    34861 cacgcgcgcg cacggtcctt gttccactac ttccccttgt cgcagtacga taacctgatg
    34921 acataacgcg cggacaacgt gcaaatcgtg gctgataaac aaataggcca gttgatgctt
    34981 ttgttgcaat gatttcaata gcctcaatat ttgcgcctgt accgttttgt cgagtgatga
    35041 tgtcggttca tcaagtatga tcagcgaggg cttaagaatt aatgccctgg caatcgcaat
    35101 acgttgtcgc tgaccaccag agaactccgc cggataacgg tggcgtgttt caggatctaa
    35161 tcccacttca tgcatcacgg ctatcacttg ttgttcgcgt tgtgcggcag aaagcgtcgg
    35221 ctggtgaacc cgtaagcctt cctcaataat ctgcaaaacg ttgagtcgtg ggttgagcga
    35281 ggagtttgga tcctgaaata ccacctgaat gcgatgacga ataggtaaca gctggcggcg
    35341 atttaaattt tgcagtggct gaccgtcaaa gatgatgctg ccctgagaat taatcagtcg
    35401 cagcagcgcc agtcccgtcg tacttttccc ggaaccggac tcgcccacta aacccagtgt
    35461 ttcacccgct cgtagcgtaa aactgatgtt tttcaccacc acattatgat ccacaatgcg
    35521 cttcaaaatc cctttgcgaa tggggaaggc aacctgaagc tgttcaacat ccagcaacgt
    35581 tgaggcaggt tctggcaacg gcactggatc gcctgacggt tcactgttga gtagcttttg
    35641 tgtgtaagga tgagtgggtg atgcaaatag cgtagcggcg taattttgct cgacacagcg
    35701 accgttttgc attaccgcca cgcggtgggc cagttttctg acaatgctga ggttatgagt
    35761 aataaacagc atgcccatat tcagctcgcc ttgcagttcg cgcaacagct gtaaaatctg
    35821 cgcctggaca gagacgtcca gtgcggtggt cggttcatcg gcaattaata attccggtcg
    35881 cgttaacagc gccatcgcaa tcatcacccg ctggcgttcg ccgccggaga gctgatgcgg
    35941 ataatctgtc agccgttttg ccgcctggcg gataccaacg cgatcaaggc agttaagaat
    36001 ttcgccacga gccgcttccc gacgcatccc gcggtggagt gaaagcactt cataaagctg
    36061 tttttccagg gtatgcaatg gatttaacga caccatcggt tcctgaaaaa tcatggcgat
    36121 cttattaccg cgtacaccgc gcaacgtttg atcgctgaca tgaagcagcg attcgccatg
    36181 aaaacgaata tcgccggaga gatattcaac cggcggggaa gggagcaggc gtaaaattga
    36241 cagcgcggta acgcttttgc ctgaacctga ctcacccacc agcgccagcg tttcgccagc
    36301 ctcaatctgt agtgaaacat cattgactac tgtacgtacg gtttgctgat ggcgaaaacc
    36361 caccgacaaa ttttcaatcg ctaacagagt ttgcgtcatg ctacaccgcc ttattaggat
    36421 caaatgcgtc gcggacggct tcaccaataa agatcagcaa agacaataat atcgccaccg
    36481 acaagaaggc ggtgatccca agccacgggg cctgaaggtt atttttccct tgtaacagca
    36541 gttcaccgag tgacggtgaa ccgagcggta gaccgaagcc gaggaaatcg agcgaggtca
    36601 gggtggttat cgaactacat aaaataaacg gtaaaaaggt gagggtcgcg accatggcat
    36661 taggcaacat atgacgcagg atgatactgc gatcgctgac gccaagcgcc tgtgccgcac
    36721 gaatgtagtc gaaattacga gtacgtaaaa actccgcccg caccacgccg accagactca
    36781 tccagccaaa caagacagta attgccagca gccaccagaa gttaggctgt acgacgctgg
    36841 aaagtaaaat aatcaaaaac agcgtcggca tccccgacca tacttcaata aagcgttgtc
    36901 cccagagatc gactttaccg ccgtaatagc cttgtagcgc ccccgccagc acgcccatca
    36961 cgctggaaca gagagtcagc atcaggccaa acagaaccga gatccgcgtg ccatagagaa
    37021 tgcgtgccag cacatcgccg ccgttggcat ccgttcccag ccagttttgc cgggaggggg
    37081 gagaagggaa gggcttatcg ttagcaaagt tgatactggt agcaccaaag cgaatcggtg
    37141 cccacagtac ccagccgtta ttttccagcc gttgtttcag ccacgggtcc tgataatcag
    37201 cctgacttgc cagcgggccg ccaaaatcgc tttcgctgta gtttttcaac aacgggaaat
    37261 accaactgcc gtcataacgt accagcaacg gtttatcgtt ggcgataagc tcagaacaca
    37321 aactcaaacc aaacaagacg aggaaaatcc ataacgacca gtagccgcgg cggttatgac
    37381 gataacgcgc ccaacgggcc tgattgacgg ggctgagtcg cgacattaac gtccctcaaa
    37441 atcaatacgc ggatcaacca gcgtatagct gatatcactg acaatattca gcagcaggcc
    37501 aatcagggtg aaaatataca aggtaccaaa cattacagga taatcgcgcg agacggtcgc
    37561 ttcgtagccc agtaagccaa ggccattgag tgaaaacatc acctcaatca gcagcgagcc
    37621 ggtaaaaaac atgctgataa acgtcgccgg aaaaccggca atcaccagca gcatggcgtt
    37681 gcggaacaca tgtttccaga gaatgttttt ctcacttacc cctttcgcac gcgcggtcac
    37741 cacgtattgc ttgcgcactt catcaaggaa tgagtttttg gtcagcatgg tcagcgccgc
    37801 aaagccacca atcactgtcg ccagcaccgg tagcgtgata tgccacagat aatcggtgat
    37861 tttctgatac cacggcagcg aatcaaagtt agcggaaacc aggccgcgta gagggaacag
    37921 gtcgaaatag ctgccgcctg cgaagaagac aatcagcagg atggcaaaca aaaaggccgg
    37981 aatggcgtag ccgatgatga taaatgcgct actccagacg tcaaagcggc tcccattatg
    38041 aacagctttg cgaatgccta acggaatcga caccagatag ataatcagcg tgctccacaa
    38101 tccgagggtg atggaaaccg gcagactgtc tttaatcagc gtcagcaccg aggcgctgcg
    38161 aaacaggcta tcgccaaaat caaagcggat gtagtcccag agcattttga agtaacgttc
    38221 gtggatcggc ttatcgaaac cgtagcgatg agtgatctcg gcgatcactt ctggatctaa
    38281 tccgcgtccg ccacggtaat tactgtcgct gatattgccg acacccgttt gcgcatggct
    38341 ggcacgaaca ccttcaccgc ctgcgccggg taatactccg gcattaccaa actcaatggc
    38401 ggcgatggcc tggtcgaccg gaccgccagg cgcaatttgc acgatgaaaa agttgatggt
    38461 gataatcgcc cataatgttg ggatcaccag caacagacgg cgaatcaggt aagcgcccat
    38521 ctactctccc tgttgtctgg ctgacggcag tttggccgct ttattaacgt cataccacca
    38581 ggtatcgata cccaggctgt aaacagggcg tacagcgggt tgggagaatt tatcccacca
    38641 ggcgagacgg tcttccgcca tgtaccacat tggcagcatg taataattcc acgttaatac
    38701 gcgatccagt gctcgcccca acggcaataa tttttcttta tttccctgcg cggcaataat
    38761 ttggttgatc agcgagtcga taaccgggct ttgcacgccg ggggcattat aagtggaatt
    38821 gatatattcc gatgaccagg aaatctgtaa atcggaactg ggccacggca tcgcccgcca
    38881 taggcgcggc atcatgtcat agtcgcgact gcgcatgcgg ttggtgattt gcgagttatc
    38941 caccttgcga atgtccatgt tgatacccag ccgttgcagg ctgtgctgga acggcaatac
    39001 ccactgacta ttgctgcttg cgggaagcaa taattcaaag ctgagtggct gacccgttgt
    39061 ggcattaacg cgttgctgac ccttcagcac ccagcccgct tcgttgagaa gtttgtcggc
    39121 ttttaacagg ttgtcacgat cgtagccatc gcctttagat accggcggct ggtagatttg
    39181 tgtgaagact tctgacggta gatctttttt cattggtgcc agaagcacca gctccgcggc
    39241 gtcggggtaa tttctggccg cgtattcggt attctgaaaa taactgttcg tgcgactcca
    39301 ggcattgtaa aacaacgcct tgttcatcca ttcaaagtca aaggcgagag tgattgcttc
    39361 ccggacccgg cgatcgctga ataccggacg ttggatatta aacgccagcc agcgcgtatc
    39421 ctgggctgat tcgttctttt gctcatcttt gatgatgtat tttttatcga aatttttacc
    39481 ggtataacgc gtggcccagt ttttagcgtc gttttccata cgcaaatcaa aggcacctgc
    39541 tttaaacgct tcaaaggcga cattatcatc gaggtaataa tcgtagcgaa tggtgtcgaa
    39601 attccagcgt ccacggttta ccggtaagtt tgctgcccag taatctttca cgcgggaata
    39661 gacaatattt tgccccattt tccaggacgt aattcggtac ggaccactgg caagcggagg
    39721 cgtggcgagc ggatcgctaa gtttgtgatc cttccagtac ttttctggaa ataccggcag
    39781 cgaaaacaga ctcaacatat cttctttgct gggtttagct aactcaatgc gcacggttaa
    39841 cggtgcaatg gctttgacgg tggtgccttt gtagaccaga cgaaattgcg gcacgccttc
    39901 ggtcataaat ttttgaaaag taaactctac atcgcgggca gtaatgggcg aaccatcatg
    39961 aaaacgggcg cgcggattaa tagcgacctc cacccaggaa taatcgtcag catagcgtgc
    40021 gctttcagca atcagcgggt aataactgcc tggttcgtca tcggaagtcg taaatagcgt
    40081 gtcgtacagc tgctcggtgc gtgcgcccgg gttgccgcgc agtgcatagc ggttgaaatt
    40141 atcgaaggtg ccgagggctg acaacgttat ctgcccacct tttggcgcgg cggggttcac
    40201 ataatcaaaa tggttgaaat taaacgcgta ccggggttcg cccagcacgg caaaggcata
    40261 gctttccttg atagcctgcg cctgcgcacc gaaggtgaac agagcgataa acagcagcag
    40321 tatgcgcaca atcatctgca acggatttcc ttttactcat cattagccgc aatccggcat
    40381 gctttcacta tgaataataa gggacgtaag cataccttac cactgcggcg tatacggttt
    40441 cttcagccag cggacaaaat cgtccagcgg taacgggcga ctaatccagt agccttgcat
    40501 gaaattaacg ccgcgttcgc ttagccatcg cgcctgttcc ggcgtttcga ccccttcagc
    40561 aaccgtcagc atattgaggc gtttcgccag cgtcagcacc gcgtcaagta cgggtgaagt
    40621 gatcgtttcc gtaccgatgg cgttgataaa tccacggtca attttcagat aatcgagcgt
    40681 aaaacgctca agatagataa gcgcgctgtg cccggtgccg aagtcatcaa tagcaatttc
    40741 tacgccgacc gagtgcagcc aggcgaagag ttgtgtggct tcttgctctt tcagcatatc
    40801 gcgctcggta atttccagca caatctggaa atggtgtgcg ggtagggaag tgagcagttt
    40861 ctggatatct gctttaaagc tttcgctgtg cagatggtcc ggcgcaatgt taatgccaaa
    40921 tttgacgcct accggcagca ctttttctaa ttctgcggca tcgcgggcaa ttaactcaaa
    40981 caggtgctga gtcagcggca caatcatctt ttgcgattcg gcaaagttaa tgaaggcatc
    41041 cgggggaatt tctcccgcta caggatgccg ccagcgtagc agtacttcca ggcccgtgac
    41101 tcgtaaagct tgtgtatcca ccaccggttg atacgccacg taaaattgtt cgcgcttgat
    41161 ggcggtcatg atttctctgc cggggcgcat acgtacgctc atcaggtaat agcagagcag
    41221 gccaacgaca gttccactca tgccgcccag taaaaatgcg taccacacat cgttccatgt
    41281 ccagtcatct gcataaagcc gaacggtcag aggaatgccc gcaattttag tttcacggac
    41341 cggcatgtcg gttaattcat taacgttcat caaacgtgaa gaaaaggtag atagcgcagt
    41401 attgccaata atgagggcga cgccatcgta atcttcctgc cgtgaactat aaaagagtga
    41461 aggcgtcagg ttgagattca gagcggcaaa gacgccgcta tttttcagca aagggttgcg
    41521 ataccagatg acgattgcgg gtttgttcgg caccatcggc gtgccgggta agatcgccat
    41581 atcgacgttt ttattaatgt cgagcgccgg aatcaattca ttgagtggaa tgtccatctc
    41641 accagtcgca gatgaacaga atgttttttt atctttcacc agcacaaacg tacggacatt
    41701 catgctaaac gccgcgcgag cggtcagttc ggggttagcc tgctggcagg tatctaaggt
    41761 cagcggctga agccggtcag tagtggattt caggtcggca aaataggtat cgagatactt
    41821 ttgtacgtcg ataatcagtg tgtcgtattt gacttcgtgc ttatgccagg ccactaaaaa
    41881 ctgaaggcag ctaaccagta tcgcaatcat cacgcctgct acaatgcagg taagcaggag
    41941 cttacgtcca gaattgggag cgcgtatgaa catgattccg ttaatttcct gaaattacga
    42001 caacatttaa aagataacaa tgaccggact atagcctgga tcaagacgcg gaaacatcat
    42061 tttctctgat gaatgaaaac aaaaaagcac tgcctaagca gtgctttcag ttaactcgtc
    42121 aggatagcca agggattgca tccaaacggt ttattagctg cggctgagaa cccggcgtgc
    42181 ttcgttgtaa cgcttcttcc agtacggttc attcatgctg gaaataataa caccactgct
    42241 ggtggaagca tggacaaact gattgttgcc gatataaata ccgacatggc gtcccgttga
    42301 accggcacgg aacagaacta aatcacccgt acgcaaatta ctgcgggaaa cagatttacc
    42361 catttcctgc tgttcgtaag tcgaacgcgg aagttctaag ccaaattgct cacggaatgt
    42421 acgctgtacg aaaccagaac aatcgatacc ttttttagtg ctgccgccca gacgataacg
    42481 tacgcctttc cagtcagcat actgatccat aattcgcgat tttacgtcga cattacgaac
    42541 caggttttca aattcatcct gagaagcttg cagtgatgat gtttcactac ccactgcacg
    42601 tgtctcagga tgcatattct ttgcggtgtt atttgcacta catgcagaaa gcagaaccgc
    42661 tactgcaatc gcggggatcc cgcgcaagat atatctcaaa atcggttgag atttgaccat
    42721 gttgtttgtt ttcccttgaa gtccttaacg acaaatatcg ttataaaaaa atgccaagcg
    42781 acacgagact aattacctgg gcatgttgaa acaattcctt aaggccaaat ctgtggacta
    42841 acgcacaaaa ttatatgctg tgaggataat ctaccgccga ggcaaaacga gttcgagatt
    42901 acccgattgg tgcggtcttg gcgagcggtt ttacatgatt ttttataaga aaaagtgata
    42961 cagaaagtta ataagcggga ttggtcaatt aaagtacgat tgagtgaaac gcatgcaatc
    43021 cattcattta cttagagaat gatttttgcg cgcgatggat gaaagcacgg tgcgcatcat
    43081 caggattatc ctcactataa aaataagcct gatgatgtta attactgtga gttatttgtt
    43141 ttggaaatgt ttgttttttc ccggtagtga tttgtcaaaa aaggtgatta atcgatcact
    43201 taatggcgtc agcaacaccc agggcaaacc gatcaatatc acggtcatcg aaccgacaat
    43261 gatgtcagta aaccagtgtg cgccaatcat tactctggga aatgcaaaaa ccacaaaaat
    43321 aataagggcg ataaggcctg caactttgcc gaaataacgc cacatgaatg ccgaaaaaat
    43381 aagcagcatc atgccgtgat cgccgggaaa gctatctcgt gaggcatctt tcgtgggaac
    43441 agagagcagt tcgctgacgc ggttaatatc ggtaaaagtc aatgttgggc tggcccgttt
    43501 tacaggaatt aatgcctgac ccagctggtt taataccact gcagttaata gcatgaccag
    43561 acccataatc acgatacgtc gtctgccagg ggcgttttct ttcagccaga aactcagcat
    43621 caacattccc atcgccagca gagaacaacc gtcgaaagcg cgattgttgg tcaatgcaac
    43681 cagccacaaa aaggccttac tttcgaccag tttctgatta aagaaataaa aaatatccgc
    43741 atcaatcggc aaccagaatc catgattaac gggaatatac caggaaagaa acagcgcgag
    43801 gccgacaata ttcaacaaca ctatttgcgg caaattttta atcatatttt ctctaattat
    43861 cttactgaaa aacgcaggca accttacgcg gtagtcgcta aacgaagctt caacagcgcg
    43921 ctctgcaaag cattccagtc agcttcgctg ctggaaatca gctcaatacg gctgtccggt
    43981 ggcgcaacgt tttgcgtttc aatgtgcagg tcatcgccct gacggttgat tcgtaccagc
    44041 ccttcgggaa tacgcagcac gcctttgacg cgttccaccg gtgcaagtcg cgcccattcc
    44101 agaatgccaa tggtgtcgaa taccgtatca gcgtcgaata tccagccgca ggcctgatat
    44161 ccttgcccgc tgttcagact gcgacgccag cgttgatgct ctggcaggct taacgctgct
    44221 aacccttttt tcacgacatg ctgatgagaa tgcgcggcgc tggcgggcaa ctcggctaaa
    44281 ttgcgacgcg gcaaatccag aagatgaccg tcaactttcc catgctcact gtgaattaat
    44341 tgtcgatcgc caccattttg ctgccaccaa cgctgtagcg cttgctcact ttcgggcgtc
    44401 gcacggtcgg atttattggc gacaatgatg tctgcggcag ccagctggtc acggaagttt
    44461 tcattgctgg cgcttttttc gtccagcagc aagcgcggat cgagaatgca caaggtggcg
    44521 cgcagatcta tccacggttc atagactggt gcggttaaca gatcgaggat ctgtttcgga
    44581 tggcccagcc cggtcggctc tatcaacaag cggtctggtt ttccctgacg cagtaaggta
    44641 ttcaacccta cctgcatggg taaaccatta acgcagcaca tgcagccgcc ggggatctct
    44701 ttcagcaatg cgccgctatc ggcgagcaaa gcaccatcaa ttccgacttc cccaaattca
    44761 ttaaccagta ccgcccattt ttcgttggga tctttatggg ctaacagatg aagaatcgac
    44821 gtggttttcc cgctgccgag aaaaccggtg atgaggttgg tcctggtcac aacggctccg
    44881 caatactctg gtaagttaac tgagcgttaa tcctggctaa aaaaggcaga aaagagaagg
    44941 ggagagcgga cgaaggtgag cttcatccgc tttaaccgca gaaatcgtta aattttgagg
    45001 gtgtttaata gcgcctgatg tgcgccgaat tgtacgattt gttgccaggc ttgttcgatg
    45061 gcctgcacaa aatgcgggtt atctggcaga tcatcaccga aaacttcacg cagggaaagc
    45121 agggcggtta cgcgttgttc actgctgctg cccgcaacaa gttcgcgaat tttatcgcta
    45181 agcggatcgc gaacatcaat ggcatttccg gcatcatcaa cgccgctgac gtaacgcatc
    45241 cagccagcaa cgcctaatgc cagcaacgac cagtccgttt cgcaccccag atgtatgcga
    45301 atacctgcca gcatacgttg cggtaatttc tggctaccat ccattgcgat ttgccaggtc
    45361 ttatgtttca gcgccggatt agcaaaacgt gcaattaact tgtccgcata ttgtgttaaa
    45421 tcgacatctt taatttgtag tgtcggcgct tgctcattca gcattaatgt tctggcggca
    45481 tggcgaaatg cgcgatcctg catgcaatca ctgatatggg cgaatcctga aaggtaaccc
    45541 agataagcga gaaaagagtg gctgccatta agcatacgca gtttcatctc ttcccatggc
    45601 aggacatcat tcaccatttg tacacctgcg acttcccagg caggacgccc agcgacgaag
    45661 ttatcttcca ccacccactg gataaacggt tcgcagctaa tcgcgcaggg atcatttacc
    45721 cccagatgct ggctgatttc tgccagtgat tcgtcggttg cagccggaac aatgcggtcg
    45781 accatggttc ccggaaaact gacgtgttct tttatccacc cggcgagttc tggcgaacgt
    45841 ttttctgcca ttcccagcac cgcgtttttc accacatggc cattgtcggg aatattgtcg
    45901 caggagagca cggtaaacgg tgtaaggccg cgctcacggc ggcgtttcag tgcttcgacg
    45961 agaatacccg gtgcggagtg aggttcttca ggggtttgta gatcgtgaat aatccgcgga
    46021 ttactggtgt cgagtgcacc ggtggccggg tcaatacaat agcctttttc ggtaatcgtc
    46081 agggaaacaa ttgccacctg tggctcgcaa aatttctcaa taattgccgc taaggaatcc
    46141 agttttgcat taaggcattc gtgaacggca ccgacaatta tcacctgatt gccgtccgca
    46201 cctttttcca gcacggtata taaatggttc tgtgcgcgga gctggctcat cagttgatca
    46261 ccgctgaaca agctgatttc acagatcccc cagtcgccgc cctgggcatt cagcacacga
    46321 tcggtcagta acgcctgatg agcgcggtga aaggcgccaa agccaaaatg aacgatacgt
    46381 gattgcaact gctggcgatc gtagcgtgga gcgtgtacat gatgcgggag cgtaacggag
    46441 gcaattgtct tcataggagt cacgtcttag taacgataaa ttaacaatga cagtgtaaag
    46501 ttatatgaca gcaattgaaa ttggtctgga ttgttaatgc gatgaagtgt gagggcgatc
    46561 aatcaacgag tgaaaattga gggcgtaagg tgttcaggaa gcgcaaaata gaagattggt
    46621 ataataaatt aaaaaaggga cagcctgagc tatccctttt ctgcatgcgg ctgaagttag
    46681 tcagcacgcc ccatataacg gcgttcttcg atatggatac gaattttttc gcccgggctt
    46741 aagtattctg gtacctgaat caccagacca gtgctcaatg tcgccggttt gttacgggcg
    46801 ctggcagatg cccctttgat gcctggtgcg gtttcaacga tttccagatc aacggtctgc
    46861 ggcagctcaa gcgccagcag ttgaccgtcc caggtcagca cctgcatgtc cggcatgccg
    46921 ccctctggca taaacagcaa ctcttcttca atctgatctt tggtgaaggt atacggggta
    46981 tagtcttctt tatccataaa gacatattcg ttgccatcga cataggagaa gtcaacgtaa
    47041 cggcgggtca gcgtcacggt gtcaacgata tcatcacctt tgaaacgctc ttctactttc
    47101 agcccggtac ggacatcaga aaaacgcatt ttgtacagcg tagcggcacc gcgggcagtg
    47161 ggcgactgaa tatcaatatc cttaaccagc agcagtttgc cgttgtaatt cagtaccata
    47221 ccttttttaa tttcgttcgc tcttggcatc gaaaaattcc tgtagtcgaa aatgtcaaaa
    47281 atatcgcgac aaagtactcg cgacgggcct ttcgggcaag tggtattcgc acttttgctg
    47341 gtgcaaaaaa ggtggtactg tgcgcgctca tcaatccggt ggttaacctt aagagaacaa
    47401 cgtatgaaat gccgtccggg ttgcggggcg tgttgcactg ccccttcaat ttccagccct
    47461 attcccggta tgccagatgg caagcccgcc aatacgccct gcattcagct tgatgaacag
    47521 caacgctgta aaattttcac ctcgccactg cgaccgaaag tctgtgcagg cttacaagcc
    47581 agcgctgaaa tgtgcggtaa ctcgcgtcag caggccatga cctggcttat cgatctggaa
    47641 atgctgaccg cgccttaaac atctttaatc cgcagtaagc aaaacagagt ggcgataatc
    47701 atcaccatcg caaaccagaa cacagcgtga taattccaga tctcggcgac gatgcccgcc
    47761 actgatcctg cgatgatcca gcccacgcgc gacgtgttgg tatagagcgt ggtggctgaa
    47821 cccgcctgac cgggcatcag atcctgaaaa tagagcatcc cgatgccgcc cagaatgcca
    47881 ataaaaatag catttagcag ctgcaagccc aacagaatga caggtgaatg cgccatcagc
    47941 attcctgcgt aaaaacagac gccacccacg gcagcaacgc gcattaagaa acgcttaccc
    48001 agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga tttccagccc ggcggcggtc
    48061 cccatcatca caccggccag tttctcggga agatgcagtt cgttgataat aaatagcggc
    48121 atgttgatga tgtacaggct gttcgagccc cacatcaatg tacaaatgac aaacagcagc
    48181 agcgtatcgc gacggttacg gcgcggcgct tcgatcgtgc cggtcgccag cggaagctct
    48241 ttttgcatcg acggtaaaaa cagccacacc atcacaccgc aaacaataaa cgctaccgct
    48301 gcgctcagat acattaccgt aaagctgaaa cccatcgcta acgcataagc cagcggtggg
    48361 ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag agctgaacat caccgcctca
    48421 cgtccggttt tgtcggcatg ttcacgggca agggcaaaca tttgcgggtt agcggtcgag
    48481 ccaaagctgc taagaaagac gccaacgaat agcaaaacaa agtagttgcg attccaggca
    48541 aaaagggtgc aggccagcac gcctaacagg cagcaaaaga caatcagcga tttgcgatcg
    48601 ccgcgcttat cagagcgccc ggcgagaaac tgacttacca gaatcccaat gacagcgctg
    48661 ccggtaaaga agaatcccac catcgccgga cgggcatgta cttcatcggt aagaaaaata
    48721 ctgagtgtcg gggtttgcag agcgcccgca ataccggtga gaaaggcaac gattaaaaac
    48781 gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg cgggggagtt atgcatgctg
    48841 gtcgatcact ggaagaggaa gacgcggagt ttacgctgct tgtcagaaaa taaacagaca
    48901 gcgaaatcaa aagcgccacg aataatcaaa atgccatttc actgttgctg attggctgct
    48961 gacgctgctg aaatgaaatt gctgataacg gattttccca tcagcaatta ggaaaaatgg
    49021 caaaaaattg tgcagcacat caaacttttg ctcataactt tacggctttc cttgcgtgct
    49081 gaaacagaag tattatgctt tcttgaaacg tttcagcgcg atcttgtctt taaccctaag
    49141 ccaggttggc gctttttttc tcatagaggc tgaatcgttt caattcagca agagaggaga
    49201 actatgttcc agttatccgt acaggacatc catccgggcg aaaaggccgg agacaaagaa
    49261 gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg gtaatgtagc agaaggctac
    49321 gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt tcctcggcaa tggtattgct
    49381 attccacacg gcactaccga cacccgcgat caggtgctga aaaccggcgt tcaggtattt
    49441 cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag cgtacgtggc aatcggtatt
    49501 gctgccagct cggatgaaca tctgggcctg ctacgccagc tgacccacgt actgagcgat
    49561 gattccgttg ctgaacaact gaagtcagca acaacagcag aagaacttcg cgcattactg
    49621 atgggcgaaa agcagagtga gcagctgaag ctcgacaacg aaatgctgac gctggatatc
    49681 gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg cgcgtctgaa agaggcgggg
    49741 gcagttgacg ccactttcgt caccaaagcc atcaatgaac aaccgctgaa tctcggacag
    49801 ggtatctggc tgagcgatag cgccgaaggc aatctgcgta gcgcgattgc ggtaagccgt
    49861 gcggcaaatg cttttgatgt ggacggcgaa acggcagcca tgctggtgag tgtggcgatg
    49921 aatgacgatc agcccattgc ggttcttaag cgtctcgctg atttattgct cgacaataaa
    49981 gctgaccgct tgctgaaagc ggatgcggca acgttgctgg cgctgctgac cagcgatgat
    50041 gcgccgaccg acgacgtgtt aagcgcggag tttgtggtgc gcaatgaaca cggcctgcat
    50101 gctcgtccag gtaccatgct ggtcaatacc attaaacaat ttaacagtga tattaccgtg
    50161 acaaaccttg atggtaccgg caaaccggca aacggacgta gtctgatgaa agttgtggca
    50221 cttggcgtta agaaaggtca tcgcctacgc tttaccgccc agggtgcaga tgctgaacag
    50281 gcgctgaaag caatcggcga cgctatcgct gctggtcttg gggagggcgc ataatgagca
    50341 gacgtgttgc tactatcacc cttaatccgg cttatgacct tgttggtttc tgcccggaaa
    50401 ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct gcatgcggcg ggtaaaggca
    50461 tcaacgtggc caaagtatta aaagacctgg gaattgatgt caccgttggc ggcttcctgg
    50521 gtaaagacaa tcaggatggt tttcagcaac tgttcagcga gctgggcatt gccaaccgtt
    50581 tccaggttgt acaggggcgc acccgaatta acgttaagct gacggaaaaa gacggcgaag
    50641 tgaccgactt caacttctcg ggttttgaag tcacccccgc cgactgggaa cgctttgtga
    50701 ctgattctct gagctggctc ggtcagttcg atatggtctg tgtcagcgga agcttaccgt
    50761 caggcgtcag cccggaagcg ttcaccgact ggatgactcg cctgcgtagt cagtgtcctt
    50821 gcattatctt tgatagtagc cgtgaagcgt tagtagcagg tttgaaagcg gcaccgtggc
    50881 tggtgaaacc taaccgccgc gagttggaaa tctgggcagg ccgtaaactg cctgaaatga
    50941 aagatgtgat tgaagctgca catgcgctac gtgaacaagg tattgcgcat gttgttattt
    51001 cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg cgaatggatc gccaaaccac
    51061 cgtcagtcga tgtcgtaagc accgttggcg caggggattc tatggttggt ggcctgattt
    51121 atggcttgct gatgcgtgaa tccagtgaac acacactgcg tctggcgaca gctgttgcag
    51181 ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg tccgcagttg gccgcaatga
    51241 tggcgcgcgt cgacttacaa ccttttaact gacagcagga gaggcataat gaaaacgctg
    51301 ctgattattg acgctaatct cggtcaggca cgcgcctata tggcgaagac cctgctgggc
    51361 gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc cgaacgacgc agaaatggcg
    51421 attgttctcg gtgattccat cccgaatgac agcgcgctga acggtaaaaa tgtctggctg
    51481 ggtgatattt cccgggcagt tgcgcaccct gagctgttcc tgagtgaagc caaaggccat
    51541 gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg ttgccgccag cggtccgaaa
    51601 cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac acacctttat ggcggctgaa
    51661 gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga aagttgaaac ccgtggttct
    51721 gttggcgcgg gtaatgcaat cactcccgag gaagtagccg cagcggatct ggtgattgtg
    51781 gcggcagata tcgaagtgga tctggcgaaa tttgctggta aaccgatgta tcgtacctct
    51841 accggtctgg cgctgaagaa aaccgcgcag gaactggata aagcggttgc tgaagcaacg
    51901 ccgtatgaac cggcgggcaa aactcaaacg gcgaccactg aaggtaagaa agagagtgca
    51961 ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc tgccgatggt cgttgctggt
    52021 ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag cgtttaaaga gccgggtacg
    52081 ttggcagcgg cgctgatgca gattggtggt ggttcagcct ttgcgctgat ggtgccggta
    52141 ctggcaggtt atattgcctt ttccattgcc gatcgtccgg gcctcacgcc gggtctgatt
    52201 ggcggtatgc tggcggtcag caccggttct ggcttcattg gtggtattat tgcgggcttc
    52261 ctggctggtt acattgcgaa gttaatcagt acgcaattga aactgccaca gagtatggag
    52321 gcgctgaaac cgatcctgat cattccgcta atttccagtc tggtggtcgg tctggcgatg
    52381 atctacctga tcggtaaacc ggttgctggc attctcgaag ggttgactca ctggctgcag
    52441 accatgggga ctgcgaatgc ggttctgctg ggggcgatcc tcggtggcat gatgtgtact
    52501 gacatgggcg gtccggtaaa taaagcagcg tacgcattcg gtgtgggtct gctgagtact
    52561 caaacctatg gcccgatggc ggcgattatg gcggcaggta tggtgccacc gctggcaatg
    52621 ggcctggcaa caatggtggc gcgtcgcaaa ttcgacaaag cgcagcagga aggtggcaaa
    52681 gccgctctgg tactgggact gtgctttatt tcggaaggtg caattccgtt tgctgctcgt
    52741 gatccgatgc gtgtgctgcc gtgctgtatc gtgggtgggg cgctgactgg cgc
//
LOCUS       DAEVZA010000016        52311 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEVZA010000016 DAEVZA010000000
VERSION     DAEVZA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.016"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1737)
                     /gene="crfC"
                     /locus_tag="LAF92_001007"
     CDS             complement(<1..1737)
                     /gene="crfC"
                     /locus_tag="LAF92_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBI9790055.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQARGYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVF"
     gene            2139..2474
                     /locus_tag="LAF92_001008"
     CDS             2139..2474
                     /locus_tag="LAF92_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBI9790056.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            2634..3077
                     /gene="yjdN"
                     /locus_tag="LAF92_001009"
     CDS             2634..3077
                     /gene="yjdN"
                     /locus_tag="LAF92_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBI9790057.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            3210..3998
                     /gene="phnC"
                     /locus_tag="LAF92_001010"
     CDS             3210..3998
                     /gene="phnC"
                     /locus_tag="LAF92_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBI9790058.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            4023..5039
                     /gene="phnD"
                     /locus_tag="LAF92_001011"
     CDS             4023..5039
                     /gene="phnD"
                     /locus_tag="LAF92_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI9790059.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            5101..5880
                     /gene="phnE"
                     /locus_tag="LAF92_001012"
     CDS             5101..5880
                     /gene="phnE"
                     /locus_tag="LAF92_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBI9790060.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            5901..6626
                     /gene="phnF"
                     /locus_tag="LAF92_001013"
     CDS             5901..6626
                     /gene="phnF"
                     /locus_tag="LAF92_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBI9790061.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            6627..7079
                     /gene="phnG"
                     /locus_tag="LAF92_001014"
     CDS             6627..7079
                     /gene="phnG"
                     /locus_tag="LAF92_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBI9790062.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            7076..7660
                     /gene="phnH"
                     /locus_tag="LAF92_001015"
     CDS             7076..7660
                     /gene="phnH"
                     /locus_tag="LAF92_001015"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBI9790063.1"
                     /translation="MTLETAFILPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            7660..8724
                     /gene="phnI"
                     /locus_tag="LAF92_001016"
     CDS             7660..8724
                     /gene="phnI"
                     /locus_tag="LAF92_001016"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBI9790064.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            8717..9562
                     /gene="phnJ"
                     /locus_tag="LAF92_001017"
     CDS             8717..9562
                     /gene="phnJ"
                     /locus_tag="LAF92_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBI9790065.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            9559..10317
                     /gene="phnK"
                     /locus_tag="LAF92_001018"
     CDS             9559..10317
                     /gene="phnK"
                     /locus_tag="LAF92_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBI9790066.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            10428..11108
                     /gene="phnL"
                     /locus_tag="LAF92_001019"
     CDS             10428..11108
                     /gene="phnL"
                     /locus_tag="LAF92_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBI9790067.1"
                     /translation="MIYVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            11105..12241
                     /gene="phnM"
                     /locus_tag="LAF92_001020"
     CDS             11105..12241
                     /gene="phnM"
                     /locus_tag="LAF92_001020"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBI9790068.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            12241..12798
                     /gene="phnN"
                     /locus_tag="LAF92_001021"
     CDS             12241..12798
                     /gene="phnN"
                     /locus_tag="LAF92_001021"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBI9790069.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            12785..13219
                     /gene="phnO"
                     /locus_tag="LAF92_001022"
     CDS             12785..13219
                     /gene="phnO"
                     /locus_tag="LAF92_001022"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBI9790070.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            13221..13979
                     /gene="phnP"
                     /locus_tag="LAF92_001023"
     CDS             13221..13979
                     /gene="phnP"
                     /locus_tag="LAF92_001023"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBI9790071.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(14126..14455)
                     /gene="yjdP"
                     /locus_tag="LAF92_001024"
     CDS             complement(14126..14455)
                     /gene="yjdP"
                     /locus_tag="LAF92_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBI9790072.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDYGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(14524..14841)
                     /locus_tag="LAF92_001025"
     CDS             complement(14524..14841)
                     /locus_tag="LAF92_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HBI9790073.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            15094..17079
                     /gene="yjcS"
                     /locus_tag="LAF92_001026"
     CDS             15094..17079
                     /gene="yjcS"
                     /locus_tag="LAF92_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBI9790074.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            17288..17563
                     /locus_tag="LAF92_001027"
     CDS             17288..17563
                     /locus_tag="LAF92_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790075.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            17582..18613
                     /gene="mdtN"
                     /locus_tag="LAF92_001028"
     CDS             17582..18613
                     /gene="mdtN"
                     /locus_tag="LAF92_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBI9790076.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPFEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            18613..20664
                     /gene="mdtO"
                     /locus_tag="LAF92_001029"
     CDS             18613..20664
                     /gene="mdtO"
                     /locus_tag="LAF92_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBI9790077.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            20661..22127
                     /gene="mdtP"
                     /locus_tag="LAF92_001030"
     CDS             20661..22127
                     /gene="mdtP"
                     /locus_tag="LAF92_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBI9790078.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            22325..24472
                     /gene="fdhF"
                     /locus_tag="LAF92_001031"
     CDS             22325..24472
                     /gene="fdhF"
                     /locus_tag="LAF92_001031"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:22742..22744,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBI9790079.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            24566..25255
                     /locus_tag="LAF92_001032"
     CDS             24566..25255
                     /locus_tag="LAF92_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI9790080.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(25333..26646)
                     /gene="gltP"
                     /locus_tag="LAF92_001033"
     CDS             complement(25333..26646)
                     /gene="gltP"
                     /locus_tag="LAF92_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBI9790081.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(26988..27584)
                     /gene="nrfG"
                     /locus_tag="LAF92_001034"
     CDS             complement(26988..27584)
                     /gene="nrfG"
                     /locus_tag="LAF92_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBI9790082.1"
                     /translation="MKQPKVPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(27581..27964)
                     /gene="nrfF"
                     /locus_tag="LAF92_001035"
     CDS             complement(27581..27964)
                     /gene="nrfF"
                     /locus_tag="LAF92_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBI9790083.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(27957..29675)
                     /locus_tag="LAF92_001036"
     CDS             complement(27957..29675)
                     /locus_tag="LAF92_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBI9790084.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(29695..30651)
                     /gene="nrfD"
                     /locus_tag="LAF92_001037"
     CDS             complement(29695..30651)
                     /gene="nrfD"
                     /locus_tag="LAF92_001037"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBI9790085.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(30648..31319)
                     /gene="nrfC"
                     /locus_tag="LAF92_001038"
     CDS             complement(30648..31319)
                     /gene="nrfC"
                     /locus_tag="LAF92_001038"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBI9790086.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(31316..31882)
                     /gene="nrfB"
                     /locus_tag="LAF92_001039"
     CDS             complement(31316..31882)
                     /gene="nrfB"
                     /locus_tag="LAF92_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBI9790087.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(31927..33363)
                     /gene="nrfA"
                     /locus_tag="LAF92_001040"
     CDS             complement(31927..33363)
                     /gene="nrfA"
                     /locus_tag="LAF92_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBI9790088.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            33755..35713
                     /gene="acs"
                     /locus_tag="LAF92_001041"
     CDS             33755..35713
                     /gene="acs"
                     /locus_tag="LAF92_001041"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBI9790089.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            35914..36228
                     /locus_tag="LAF92_001042"
     CDS             35914..36228
                     /locus_tag="LAF92_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBI9790090.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            36225..37874
                     /gene="actP"
                     /locus_tag="LAF92_001043"
     CDS             36225..37874
                     /gene="actP"
                     /locus_tag="LAF92_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBI9790091.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            38052..39344
                     /locus_tag="LAF92_001044"
     CDS             38052..39344
                     /locus_tag="LAF92_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBI9790092.1"
                     /translation="MRYNGLNNMFFPLCLINDNHSVTSPSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKHFIDIALYANNK
                     CDDRITRRVYLSKEKEVSIKVVYFINNVAVHNNTIEIPQTVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDDCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     RQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(39498..41147)
                     /locus_tag="LAF92_001045"
     CDS             complement(39498..41147)
                     /locus_tag="LAF92_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBI9790093.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(41299..42648)
                     /gene="ghxP"
                     /locus_tag="LAF92_001046"
                     /gene_synonym="yjcD"
     CDS             complement(41299..42648)
                     /gene="ghxP"
                     /locus_tag="LAF92_001046"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBI9790094.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(43194..43658)
                     /gene="soxR"
                     /locus_tag="LAF92_001047"
     CDS             complement(43194..43658)
                     /gene="soxR"
                     /locus_tag="LAF92_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBI9790095.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            43744..44067
                     /gene="soxS"
                     /locus_tag="LAF92_001048"
     CDS             43744..44067
                     /gene="soxS"
                     /locus_tag="LAF92_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBI9790096.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(44070..45656)
                     /gene="pdeC"
                     /locus_tag="LAF92_001049"
     CDS             complement(44070..45656)
                     /gene="pdeC"
                     /locus_tag="LAF92_001049"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBI9790097.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            46086..46367
                     /locus_tag="LAF92_001050"
     CDS             46086..46367
                     /locus_tag="LAF92_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBI9790098.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(46466..47002)
                     /gene="ssb1"
                     /locus_tag="LAF92_001051"
     CDS             complement(46466..47002)
                     /gene="ssb1"
                     /locus_tag="LAF92_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBI9790099.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            47256..50078
                     /gene="uvrA"
                     /locus_tag="LAF92_001052"
     CDS             47256..50078
                     /gene="uvrA"
                     /locus_tag="LAF92_001052"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBI9790100.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(50113..50469)
                     /locus_tag="LAF92_001053"
     CDS             complement(50113..50469)
                     /locus_tag="LAF92_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBI9790101.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(50473..50889)
                     /locus_tag="LAF92_001054"
     CDS             complement(50473..50889)
                     /locus_tag="LAF92_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBI9790102.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(51000..51713)
                     /gene="aphA"
                     /locus_tag="LAF92_001055"
     CDS             complement(51000..51713)
                     /gene="aphA"
                     /locus_tag="LAF92_001055"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBI9790103.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            52115..>52311
                     /locus_tag="LAF92_001056"
     CDS             52115..>52311
                     /locus_tag="LAF92_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790104.1"
                     /translation="MNISYVNSSKTTSLPIELDALNNKDISYAKDFSYAKDFFLYIET
                     QLKIAKDFCRPGEEVSSSIAS"
BASE COUNT        12722 a        14301 c        13541 g        11747 t
ORIGIN      
        1 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
       61 ttgctcaatg ggccgaataa catcggttaa cacccggcaa agggtggatt caagctcggc
      121 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
      181 gcaggtacga atctcgctta acggctccga atcatcctga aacaaggcgg ctaactgcgc
      241 attcagcgca tcttgctgtt gacgcagaaa gtggttggcg gaggtcaggg ccagctcgat
      301 ttcatgttta acctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
      361 ttcgacctga tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
      421 aaaatccagg tatccccgcg cctgctgcgc gtaattcaac agtttatgcg cagcagatcg
      481 cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc
      541 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag
      601 atcgtcatga cgccagcgcc tgccgagcgc agcatgggca aaatcttcca cccagcgttg
      661 ttgctctggc gctggtaact taccgttgtt ggctaactca tggcgcgctc gattcgccag
      721 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag
      781 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
      841 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg
      901 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
      961 ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
     1021 cccgggggta tccagtaacg tcaactgacc gggataactt tccagccccg ccagatggac
     1081 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
     1141 gacgtccagc gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat
     1201 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
     1261 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
     1321 caggcgctgt tgtaattttt gaattaaacg gtcgattggc gcgacatgtg aaaaatgcag
     1381 taccggttct ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg
     1441 attacgatta ggcaaaacct ccgtaccaac aatggcatta atggtggttg atttccctgc
     1501 tttcatggta ccgacaatcg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
     1561 attcagcatc gcgtgacgtt cggcgatatt aggctgactc cagggtaaag ccagttgtgg
     1621 cgcatcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
     1681 aagctgtaac aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga
     1741 aaattcttcc ttaaagcaaa ttttgttact tttatttagc cagattgttt ttgagttctg
     1801 ttttcggctt ttataattac tgcaagaaat aattttatat ttagcgtgtt gttttttatt
     1861 agaataaata acgtcttccg atacgtttaa aacgtcagaa agataaaaat atcatgtgaa
     1921 taaaaaaaag aacaagtaga gcattaacat tatcttaaat aataaataga ggcaaaaaga
     1981 ttattttctt tttgcgtttc ctttcaaatg aaaacgatcg tcgtctaaaa tcagcagtac
     2041 ccccgacaaa ctcagggatt ttgtgtataa ttgcggcctt tttcggcaat ctgccgtttt
     2101 ttggcgcttt tgccctgctg acttttgagg aaatccacat gtcattacca cactgcccaa
     2161 aatgcaactc cgaatacact tacgaagata acggcatgta catctgcccg gaatgtgcct
     2221 acgaatggaa cgacgcagaa cctgcacagg aaagcgacga gctgatcgtt aaagatgcta
     2281 acggcaatct gctggctgac ggcgacagcg ttaccatcat taaagatctg aaggtgaaag
     2341 gtagctcttc gatgctgaaa attggcacca aagtgaaaaa catccgcctg gttgaaggcg
     2401 accataacat cgattgcaaa atcgacggtt ttggtccgat gaaactgaaa tctgagtttg
     2461 tgaaaaagaa ctgattgtat tgtgatcggt aagccggata aggcgctcgc gccgcatccg
     2521 gcaacgatgc cgactgcctg atgcgacgct gaccgcgtct tatcaggctt ctctctcatc
     2581 tgtataaatt tcgaactaca cttaactggc ttctcttaac tgaggtcacc atcatgccgt
     2641 taagtcccta cctctctttt gccggtaact gtgccgacgc aattgcttat tatcaacgta
     2701 cgttgggcgc tgaactgctc tataaaatca gcttcggcga aatgccaaaa tcagcgcagg
     2761 acagcgccga gaactgccct tccggaatgc aatttcccga taccgccatc gcccacgcca
     2821 acgtgcgcat tgccggaagc gacatcatga tgagcgatgc tataccgtca ggaaaagcca
     2881 gctactccgg ctttacgctg gtgctcgatt cgcaacaggt cgaagaagga aaacgctggt
     2941 ttgacaatct tgccgctaac ggaaaaatcg aaatggcctg gcaggaaact ttctgggcgc
     3001 atggctttgg caaagtcacc gataaatttg gcgtaccgtg gatgattaat gtcgtcaaac
     3061 aacaaccaac gcaataaccc gccgggaggc ccgccctccc gcactgtcat cgaattcccg
     3121 ttaactcttc atctgttagt cacttttaat taaccaaatc gtcacaataa tccgccacga
     3181 tggagccact tttttaggga ggctgcatca tgcaaacgat tatccgtgtc gagaagctcg
     3241 ccaaaacctt caatcagcat caggcgctgc atgcggttga tctgaacatt catcacggtg
     3301 aaatggtggc tctgcttggg ccgtcgggtt ccggcaaatc caccctttta cgtcacttaa
     3361 gcggtttgat taccggcgat aaatccgccg gcagccatat cgagctgctg ggccgcacag
     3421 tccagcgcga aggccgtctg gcgcgcgata tccgcaaaag ccgcgccaac accggctaca
     3481 tcttccaaca attcaacctg gtgaaccgcc tgagcgtact ggagaacgtg ctgattggcg
     3541 cgctcggcag cacgccgttc tggcgcacct gttttagctg gttcaccggc gagcagaaac
     3601 agcgcgcgtt acaggcgctg acccgcgttg gcatggtgca ttttgcccat cagcgcgttt
     3661 ccaccctctc cggcggacag cagcagcgtg tggcgattgc ccgcgcgctg atgcagcagg
     3721 cgaaggtgat tctggccgat gaacccatcg cctcgctgga cccggaatcc gcccgcatcg
     3781 tgatggacac cctgcgcgac atcaatcaga acgacggcat caccgtggtc gtcacgctgc
     3841 atcaggtgga ttacgccctg cgctactgcg aacgcatcgt cgccctgcgc caggggcacg
     3901 ttttctacga cggcagcagc caacagtttg ataacgaacg ttttgaccat ctctaccgca
     3961 gcattaatcg catcgaagag aacgcgaaag ctgcctgaca tccccatcat tgaggaaaac
     4021 gaatgaacgc taagataatt gcctcgctgg ccttcaccag catgttcagc ctcagcaccc
     4081 tgttaagccc ggcacacgcc gaagagcagg aaaaggcgct gaatttcggc attatttcaa
     4141 cggaatcaca gcaaaacctg aaaccgcaat ggacgccatt cttacaggat atggagaaga
     4201 agctgggcgt gaaggtgaac gccttctttg ccccagacta cgcaggcatt atccagggaa
     4261 tgcgcttcaa taaagtggat atcgcctggt acggcaacct gtcggcaatg gaagcggtgg
     4321 atcgcgccaa cggccaggtc ttcgcccaga cggtcgcggc ggatggatcg ccaggttact
     4381 ggagcgtgtt gatcgtcaac aaagatagtc cgatcaacaa cctgaacgat ctgctggcga
     4441 agcggaaaga tctcaccttc ggcaatggcg atcctaactc cacctctggc ttcctcgtcc
     4501 ccggttacta cgtctttgcc aaaaacaata tctccgccag cgactttaag cgcaccgtca
     4561 acgctgggca tgaaaccaac gcgctggccg tcgccaacaa gcaggtggat gttgccacca
     4621 acaacaccga aaacctcgac aagctgaaaa cctccgcgcc agagaagctg aaagaactga
     4681 aggtgatctg gaagtcgccg ctgatcccag gcgatccgat cgtctggcgt aaaaaccttt
     4741 ccgaaaccac caaagacaag atctacgact tctttatgaa ctacggcaaa acgccggaag
     4801 agaaagcggt gctggaacgc ctgggctggg ccccgttccg cgcctccagc gacctgcaac
     4861 tggtgccgat tcgccagctc gcactgttta aagagatgca gggcgtaaaa agcaataaag
     4921 gactgaatga gcaggacaag ctggcgaaaa ccaccgagat tcaggcgcag ctggatgacc
     4981 tggaccgcct gaacaacgcg ttaagcgcga tgagttcggt gagtaaagcg gtgcagtaat
     5041 gtgctcgatc ggtcccctct cccttgaggg agagggagaa aacattaaac ggagccaaac
     5101 atgcaaacca tcaccatcgc cccacccaag cgtagctggt tctcgcttct gagctgggcc
     5161 gttgttctcg ccgtgctggt cgtctcgtgg cagggcgcgg aaatggcccc gcttacgctg
     5221 attaaagacg gcggcaacat ggcaaccttc gctgccgact tcttcccgcc cgatttcagc
     5281 cagtggcagg attacctcac cgaaatggcc gtcacgctgc aaatcgccgt ctggggcacc
     5341 gcgctggcag tggttctctc catccccttc ggcctgatga gcgccgaaaa cctggtgccg
     5401 tggtgggttt accagcccgt tcgccgcctg atggacgcct gccgcgccat taacgaaatg
     5461 gtcttcgcca tgctgttcgt ggtcgccgtc ggcctcggcc cgttcgctgg cgtgctggcg
     5521 ctgtttatcc acaccaccgg cgtgctctcc aagctgcttt ccgaagcggt ggaagcgatt
     5581 gaacccggcc cggtagaagg cattcgcgcc accggtgcca acaagctgga agagatcctt
     5641 tacggcgtgc tgccacaggt aatgccgctg ctgatctcct actccctcta tcgcttcgaa
     5701 tccaacgtcc gctcggcgac cgtcgtcggc atggtcggtg caggcgggat cggcgtcacc
     5761 ctgtgggaag cgattcgcgg tttccagttc cagcaaacct gcgccctgat ggtgcttatc
     5821 atcgtcacgg tcagcctgct ggatttcctc tctcaacggt tgcgtaagca ctttatctga
     5881 taagcgaggc attgatatct atgcacttgt ctacacatcc gaccagctac ccaacacgct
     5941 atcaagagat agccgcaaaa cttgagcagg agcttcgtca acactaccgc tgcggcgact
     6001 atcttcccgc cgagcagcaa ctggcggcgc gctttgaggt gaaccgccac accctgcgcc
     6061 gcgccatcga ccaactggtg gaaaaaggct gggtacagcg ccgtcagggc gtcggcgtgc
     6121 tggtgctgat gcgcccgttc gattacccgc tcaacgccca ggcgcgtttt agccagaacc
     6181 tgctggacca gggtagccat cccaccagcg aaaaactgct gtcggtattg cgccccgcgt
     6241 ccggtcacgt cgctgacgcg ctggggatta ccgagggcga gaacgttatc catttgcgca
     6301 ccctgcgtcg cgtcaacggc gtcgcgctct gtttaatcga ccactacttt gcggacctca
     6361 ccctctggcc gacactgcaa cgtttcgaca gcggttcgct gcacgatttt ctgcgcgagc
     6421 aaaccggaat tgcgctgcgc cgcagccaga cgcggatcag cgcccgccgc gcccaggcca
     6481 aagagtgcca gcgccttgaa atcccgaata tgtcgccgct gctgtgcgtg cgcaccctta
     6541 accaccgtga cggtgaaagc agcccggcgg agtactccgt cagcctgacg cgcgccgaca
     6601 tgattgaatt cactatggag cactgaatgc acgcagatac cgcgacccgc cagcactgga
     6661 tgtccgtact ggcgcacagc caaccggctg aactggcagc ccgcctgaac gcgctgaaca
     6721 tcaccgccga ctatgaagtg atccgcactg ccgaaactgg cctggtgcag attcaggcgc
     6781 gaatgggcgg taccggcgaa cgcttttttg ccggcgacgc cacgctgacc cgcgccgccg
     6841 tacgcctgac tgacggcacg ctcggctaca gctgggtgct ggggcgcgac aaacagcacg
     6901 ccgaacgctg cgcgctgatt gacgcgctga tgcagcaatc tcgccacttt caaaacttat
     6961 cagaaactct tattgccccg ctggacgctg accgtatggc acgcattgcc gcacgccagg
     7021 ccgaagtgaa cgccagccgg gtcgacttct ttacgatggt tcgcggagat aacgcatgac
     7081 cctggaaacc gcttttattc ttcctgtgca ggatgcccag cacagttttc gtcgcctgtt
     7141 aaaggccatg agcgagccgg gcgtgattgt cgccctgcat cagctcaaac gcggctggca
     7201 gccgctgaat atcgccacca ccagcgtgct gctgacgctg gccgataacg acacgccggt
     7261 gtggcttgcc gcgccattga gtaacgatat cgtcagccag agcctgcgtt ttcataccaa
     7321 cgcgccgctg gtcagccagc cggaacaggc gaccttcgcg gtgacggatg aggcgatttc
     7381 cagcgaacag cttaacgccc tttccaccgg caccgccgtt gcgccggaag cgggtgcaac
     7441 gctgatttta caggtcgcca gcctgagcgg cgggcgcatg ttacgtctca ccggcgcggg
     7501 cattgccgaa gaacgaatga tcgctccgca gctgccggag tgcattctgc acgaactcac
     7561 cgagcgcccg cacccgttcc cgctcggcat cgacctgatc ctgacctgcg gcgagcgcct
     7621 gctggctatt ccgcgaacca ctcatgtgga ggtgtgctga tgtacgttgc cgtgaaaggg
     7681 ggcgagaagg cgatcgacgc cgctcacgcc ctgcaagaga gccgacgccg gggcgatacc
     7741 gatttgcctg aactgagcgt cgcccagatt gaacagcagc ttaatcttgc ggtggatcgc
     7801 gtgatgaccg aaggcggcat tgccgaccgc gaactggcgg cgctggcgct gaaacaggcc
     7861 agcggcgata acgtcgaagc aattttcctg ctgcgcgcct accgcaccac gctggcgaag
     7921 ctcgcggtaa gcgagccgct cgacaccaca gagatgcgtc tcgaacggcg tatttcggcg
     7981 gtttataaag acattcccgg cggccagctg cttggcccaa cctacgacta cacccatcgc
     8041 ctgctcgatt tcaccctgct ggcaaacggt gaagcgccga cgctgaccac cgccgacagc
     8101 gaacaacagc cgtcgccgca cgttttcagc ctgctggcgc gtcaggggct ggcgaagttt
     8161 gaagaggata gcggcgcaca gccggatgat atcacccgca cgccgccggt ttacccctgc
     8221 tcacgttctt cccgtttgca gcagttgatg cgcggcgacg aaggctattt gctggcgctg
     8281 gcctactcca cccaacgcgg ttacgggcgc aatcacccgt tcgcgggcga gatccgcagt
     8341 ggttacatcg acgtgtcgat tgtgccggaa gagctgggat ttgcggtgaa cgtcggcgaa
     8401 ctgctgatga ccgaatgtga aatggtcaac ggttttatcg acccgccgga tgagccgccg
     8461 cacttcacgc gcggctacgg gctggtattc ggcatgagcg agcgcaaagc gatggcaatg
     8521 gcgctggtcg atcgtgcgtt gcaggctccg gaatacggcg agcacgcgac aggcccggcg
     8581 caggatgaag agtttgtgct ggcacatgcc gacaacgtcg aagccgcagg ctttgtctcg
     8641 cacctcaaac tcccccacta cgtcgatttc caggccgaac tggagctact caaacgtctg
     8701 caacaggaga agaaccatgg ctaatctgag cggctacaac tttgcctacc tcgacgagca
     8761 gaccaaacgc atgatccgcc gcgctatctt aaaagcggtg gcgatccccg gttatcaggt
     8821 gccgtttggc gggcgcgaga tgccgatgcc atacggctgg ggaaccggcg gcatacagct
     8881 caccgccagc gtgattggcg aaagcgacgt gctgaaggtg attgaccagg gtgcggatga
     8941 caccaccaac gccgtgtcga ttcgcaactt ctttaagcgc gtgaccgggg taaacaccac
     9001 tgaacgtacg gacgatgcga cgcttatcca gacgcgtcac cgcatccccg aaacgccgct
     9061 gaccgaagat cagatcatta tcttccaggt gccaatcccg gaaccgctgc gctttatcga
     9121 gccgcgcgaa acggaaaccc gcaccatgca cgcgctggaa gagtacggcg tgatgcaggt
     9181 gaaactgtat gaagatatcg cccgcttcgg tcatatcgcc actacctacg cctatccggt
     9241 gaaggtgaac gggcgctacg taatggaccc gtcgccgatc ccgaaattcg ataacccaaa
     9301 aatggacatg atgcccgccc tgcagctgtt cggcgcaggg cgcgagaagc gcatctatgc
     9361 ggtgccgccg tttacccgcg tggaaagtct cgatttcgac gatcacccgt tcaccgttca
     9421 gcagtgggat gagccatgcg ccatctgcgg atcgacccac agctatcttg atgaagtggt
     9481 gctggatgac gccggaaacc gcatgtttgt ctgctccgat accgattatt gccgccaaca
     9541 gagcgaggcg aaaagccaat gaataaaccg ttactttcgg tcaataacct gacccacctt
     9601 tacgcgccgg gcaaaggctt tagcgatgtc tcttttgatt tatggccggg agaagtgctg
     9661 ggcattgtcg gggagtccgg ctccgggaag accacgctgc tgaaatcaat ctccgcgcgc
     9721 ctgacgccgc agcaggggga gattcactat gagagccgtt cgctgtacgc aatgagcgag
     9781 gccgaccgcc gtcgcctgct gcgcaccgaa tggggcgtag tgcatcagca tccgctcgac
     9841 ggcctgcgcc gccaggtgtc ggcaggcggc aatatcggcg agcggctgat ggcgaccggg
     9901 gcacgtcatt acggcgatat tcgtgccacc gcgcagaagt ggctggaaga ggtggaaatt
     9961 cccgccaacc ggatcgacga cctgccgacc accttttccg gcggcatgca gcaacgtttg
    10021 cagatcgccc gcaacctggt gacgcatccg aagctggtgt ttatggacga gccgaccggc
    10081 ggactggatg tgtcggtaca ggcacgcctg ctcgacctgc tgcgcggcct ggtggtggag
    10141 ctgaacctcg cggtagtgat tgtcacccac gatttaggcg tcgcccgcct gctggcggac
    10201 cgtttgctgg tgatgaagca ggggcaagtg gtggagagtg ggttaaccga ccgcgtgctc
    10261 gacgacccgc atcatccgta tacacagctg ctggtgtcat cggttttgca gaattgagcc
    10321 ggtgccggat gcggcgtaaa cgccttatcc ggcctacaaa tgcgctcccc gtaggtcgga
    10381 taagacgcgt cagcgtcgca tccgacaccc gaaccacgag gcgaaaaatg atttacgtac
    10441 aaaacgtcag taaaaccttc atcctgcacc agcaaaacgg cgtgcgcctg cccgtcttac
    10501 aacgcgcctc gctcaccgtc aacgcaggtg aatgcgtggt gctccacggc cattccggca
    10561 gcggcaaatc aactctgcta cgctcgctgt acgccaacta tctgcccgac gaagggcaaa
    10621 tccagatcaa acacggtgac gaatgggtag acctggtcac cgcgccagcg cgcaaagtgg
    10681 tggagatccg caaaactacc atcggctggg tgagccagtt tctgcgcgtc atcccgcgta
    10741 tctcagcact ggaagtggtg atgcagccgc tgctcgatac aggcgttccg cgtgaagcct
    10801 gcgccgctaa agccgcgcgt cttctcaccc gcctgaacgt gccggaacgc ctgtggcacc
    10861 tggcaccatc gaccttctcc ggcggcgaac agcagcgcgt caacatcgcc cgcggcttta
    10921 tcgtcgacta ccccattctg ctgctcgacg aacctaccgc ctcgctggac gccaaaaaca
    10981 gcgccgcggt ggtggaactg attcacgaag ccaaagcccg tggcgcagcc atcgtcggca
    11041 tcttccatga cgaagctgta cgtaatgacg tcgccgaccg cctgcaccca atgggagcct
    11101 cttcatgatt atcaataacg ttaagctggt gctggaaaac gaggtggtgc acggttcgct
    11161 ggaggtgcag gatggcgaaa tccgcgcctt tgccgaaagc cagagccgcc tgccagaagc
    11221 gatggacggc gaaggtggct ggctactacc cggcctgatt gagctgcata ccgacaacct
    11281 cgataaattc ttcaccccgc gcccgaaagt cgactggccc gcccattcgg cgatgagcag
    11341 ccacgacgcg ctgatggtgg caagcggcat caccaccgtg ctggacgcgg tggcgattgg
    11401 cgacgtgcgc gacggcggcg atcggctgga gaatctggag aagatgatca acaccatcga
    11461 agagacgcag aaacgcggcg tcaaccgcgc cgagcaccgc ctgcacctgc gctgcgaact
    11521 gccgcatcac accacactgc cgctgtttga aaaactggtt cagcgcgaac cggtgacgct
    11581 ggtgtcgctg atggaccact caccgggcca gcgccagttc gctaaccgcg agaagtatcg
    11641 cgaatattat cagggcaaat actccctcac cgatgcgcag atgcagcagt acgaagaaga
    11701 gcaattggcg ctcgccgcac gctggtcgca gccgaatcgc gaatccatcg ccgccctgtg
    11761 ccacgcgcga caaattgcgc tcgccagcca cgatgacgcc acgcacgccc acgttgccga
    11821 atcccaccaa cttggcagcg tgatcgccga atttcccaca acgttcgaag cggcggaagc
    11881 ctcgcgtaag catggcatga acgtgctgat gggtgcgcca aatatcgtgc gtggcggctc
    11941 gcactccggc aatgtggcgg ccagtgaact ggcgcagctt ggtctgctgg atatcctctc
    12001 ttccgactac taccccgcca gcctgctgga tgcggcgttc cgcgtcgccg atgacgagag
    12061 caaccgcttt acgctaccgc aggcggtgag gctggtgacc aaaaatccgg cgcaggcgct
    12121 gaatcttcag gatcgcgggg tgattggcga gggtaaacgc gctgacctgg tgctggcgca
    12181 tcgccagggc aatcacattc atatcgacca cgtctggcgt cagggtaaaa gggtgttctg
    12241 atgatgggaa aactgatttg gttaatgggg ccgtccggct ccgggaaaga cagcctgctg
    12301 gcggaactcc gcctgcggga acaaacgcag ttactggtgg cgcatcgcta catcacgcgc
    12361 gacgccagcg ccggaagtga aaaccatatc gccctgagtg agcgggagtt ttttacccgc
    12421 gcggggcaaa acctgctggc cttaagctgg cacgctaacg gtctgtatta tggcgtcggc
    12481 atcgagattg acctctggct gcacgctgga ttcgacgtag tcgtcaacgg ttcacgcgcg
    12541 catttgccgc aggcacgggc gcgctatcaa tcggcgctgc tgcccgtctg tttacaggtt
    12601 tcgccggaga tcctgcgcca gcgcctggaa aaccgtggcc gtgaaaacgc cagtgaaatt
    12661 aacgcccgcc tggcgcgcgc cgcccgctat actccacagg attgccatac gctcaacaat
    12721 gacggcagcc tgcgccagtc ggtcgacacg ctgctgacgc tgatccatca gaaggagaaa
    12781 caccatgcct gcttgtgagc ttcgcccggc cacgcagtac gataccgacg cggtttacgc
    12841 gctgatttgt gagctaaaac aggcggagtt tgaccaccat gcgtttcgcg tgggctttaa
    12901 cgccaacctg cgcgacccaa acatgcgcta ccatctggcg ctgctggatg gcgaagttgt
    12961 cggcatgatc ggcctgcatt tgcagtttca tctgcatcat gtcaactgga tcggcgaaat
    13021 tcaggaactg gtggtgatgc cgcaggcgcg cggtctgaac gtcggcagta aattactggc
    13081 atgggcagaa gaagaagccc gccaggccgg ggccgaaatg accgagcttt ccaccaacgt
    13141 gaagcgccac gacgcacacc gtttttacct gcgtgaaggc tacgagcaga gccacttccg
    13201 cttcaccaag gcgctgtaac atgagcctga ccctcacgct caccggcact ggcggcgcac
    13261 agggcgttcc ggcatggggc tgcgagtgcg cggcctgcgc cagagcgcgg cgctcgccgc
    13321 agtatcgccg ccagccatgc agcggcgtag tgaagtttaa cgacgcgatc accctgatcg
    13381 acgccgggcg gcacgatctc accgatcgct ggtcgcctgg atcgttccag cagtttttgc
    13441 tgacgcacta ccatatggat cacgtccaag ggctatttcc gctacgctgg ggcgtgggcg
    13501 atgtgatccc ggtctacggc ccgccggatg aacagggctg cgacgatctg tttaaacatc
    13561 cgggcctgct tgatttcagc cacacagtgg aaccgtttgt ggtgtttgat ttgcaggggt
    13621 tgcaggtcac gccgctgccg cttaaccact caaaactgac cttcggttat ctgctggaaa
    13681 cggcacatag ccgggtggcg tggctgtctg acaccgctgg cctgccggaa aaaacgctga
    13741 aatttttact caataatcat ccgcaggtga tggtgattga ttgcagtcac ccgccgcgcg
    13801 cggatgcacc gcgtaatcac tgtgatttaa ataccgtgct tgcgctgaat caggttatcc
    13861 gctcgccaca ggtgattctg acccatatca gccaccagtt tgatgcgtgg ctgatggaaa
    13921 acgcactacc gtcagggttt gaggtggggt ttgatgggat ggagattggg gtggcgtgat
    13981 gagagggaat gtgcgcgctg gccccctcac cctaaccctc tccccagagg ggcgagggga
    14041 ccgattgtgc tcaatattga atattgcgct cgttttctcc ctctccccat tggggtgagg
    14101 ggcgatgcct gctccatacc caacctcatc gcccatactc atcttccatt ctccgctctt
    14161 catcctccag ttgccgacgc tcctgatcaa gctggcgctg gcgatcgtcc agctgcctgc
    14221 ggcgatcttc aaactggcgg cggcggtcgt catattgtct gcgccgatcg tcgctcactt
    14281 cacgctgcca gccgtagtcg cgcgaatctt catagtctcg cccacggtca gggttataag
    14341 cgtcattaat cgcctgctga atattaccaa tagtatcgtc gataatatca gcctgggccg
    14401 gaacggtgga cagcgtgagc agggtgaata aaagaaatag cggaaagcgt ttcattagcc
    14461 aacctcaaaa agaaactcta tccacattaa tcattactca tccatgcaag tagtggatga
    14521 atctcaattt ctccgctgct ctattgccgt aatcgcctcc acgcgttgtt gatgacgacc
    14581 gccttcgtac tgtgcgccca gccacgcatc cacaatcatt tttgccagtt cgaggccaac
    14641 cactcgtgaa ccaaaagcca gtgcaacttc actggcataa tgcggataat cagtgcgctc
    14701 tgacgacctg gtgtctttat caatcacctc actcattgac catgaataaa aggctggcac
    14761 agcaacgcag aatcctgcgg tgccagcgac atgaccgccc attgctccgg tacgtttttt
    14821 aaaaagaaac accggttgca ttgataaatt gtgctcagta tttgccgaag tacaatatat
    14881 ataataaata tataaaacac ctcatattat ttacagagat aataaattcg cgtaattttc
    14941 cctcttcagc acaaatgcct aataaaatgg ctcgttccgc tcacacttcc acttattaaa
    15001 gtcgcaattt cccaattatg ataacgccag ctttaaatga cgtattttgc atggattaca
    15061 tatttacgac acaaatgata aatggagatt tatatgaata actctcggtt attccgtttg
    15121 agcaggattg ttattgcgtt aactgctgcc agcggcatga tggtaaatac cgcttacgca
    15181 acagatgaag cgaaagccgc cactcaatat acccaacagg ttaatcagaa ttacgccaaa
    15241 tcattaccgt ttagcgatcg tcaggatttt gacgacgctc agcgtggatt tatcgctccg
    15301 ctgctggatg aaggtattct gcgcgatgct aatggcaaac catattatcg cggggaagat
    15361 tataaatttg atatcaatgc gcccgcgccc gaaaccgtta accctagcct gtggcgtcag
    15421 tcgcagttaa acggtatttc cggcctgttt aaagtcaccg acagaatgta ccaggttcgc
    15481 ggtcaggata tctccaacat cacctttatc gaaggtgata ccgggattat tgtcatcgac
    15541 ccgctggtga cgccaccgag cgcaaaagcc gcccttgacc tttacttcca aaatcgcccg
    15601 caaaaaccga ttgttgcggt tatttatacc cacagccata ccgaccacta tggcggcgtg
    15661 aaaggcatta tctctgaagc tgatgtgaaa tccggtaaag tgcaggttat cgcccctgct
    15721 ggctttatgg acgaagccat cagcgaaaac gtactggcgg gtaatattat gagccgccgt
    15781 gcactttact cctacggcct gctgctggcg cataaccctc agggtaacat cggcaatggt
    15841 cttggcgtaa cgctggcatc gggctacccg agcatcatcg caccgaacaa aaccatcacc
    15901 aaaaccggtg agaagatgat tatcgacggc ctggagtttg acttcctgat gaccccaggt
    15961 agcgaagcgc cagccgaaat gcacttctat attccggccc tgaaagccct gtgtaccgcc
    16021 gagaacgcca cgcataccct gcacaacttc tacactctgc gcggcgcgaa aacccgcgac
    16081 accagcaagt ggaccgagta tctgaacgaa acgctggata tgtggggtaa cgacgcggaa
    16141 gtcctgttta tgccgcacac ctggccggtc tggggcaata agcatatcaa tgattatatt
    16201 ggtaaatatc gcgatactat caagtacatt cacgaccaga ccctgcacct ggcgaaccag
    16261 ggctacacca tgaatgaaat cggcgacatg attaaactgc cgcctgcact tgccaataac
    16321 tgggccagcc gtggctatta cggttctgtc agccacaacg cccgcgcggt gtataacttc
    16381 tatcttggct attacgacgg taacccggct aacctgcatc cgtatggtca ggtggagatg
    16441 ggtaaacgtt acgtgcaggc gctgggcggt tctgcccgtg tcatcaacct ggcgcaagaa
    16501 gcgaacaagc aaggtgatta ccgctggtcg gcagaactgc tgaaacaggt gattgccgcc
    16561 aacccgggtg accaggtcgc gaagaatctg caagcgaata actttgaaca gctgggctat
    16621 caggccgagt ccgccacctg gcgcggtttc tacctgaccg gcgcgaaaga gctgcgcgaa
    16681 ggggtgcata agttcagcca cggcaccacc ggttccccgg acaccattcg cgggatgtcg
    16741 gtcgaaatgc tgttcgactt tatgtccgtt cgcctcgata gcgcgaaagc cgcgggtaaa
    16801 aatatcagcc tgaacttcaa tatgagcaat ggcgataacc tcaacctgac gctgaacgat
    16861 agcgtgctta actaccgtaa aacactgcaa tcccaagctg acgcctcttt ctacatcagc
    16921 cgtgaagatc tgcacgccgt gctgaccggg caagccaaaa tggcggatct ggtaaaagcg
    16981 aagaaagcca aaattattgg caatggcgcg aaactggaag aaattatcgc ctgtctggat
    17041 aatttcgatt tgtgggtgaa tatcgtaacc ccaaattaat gtcactcgct tttgggggtg
    17101 ctgaagcccc caaaagcatt tattttaaaa atatatacag ccaattaata aataaaatat
    17161 ataagttatg aatgcggtgg ctgaataaag ctatttttat tcatcgagaa aatagccata
    17221 ctgacgcctt caatggatat tgacattcac tacagatatt gttcgcagca gacaggatgt
    17281 atttaatatg ccaacagttc tctctcgcat ggcgatgcaa ctcaaaaaaa cagcctggat
    17341 aattcccgtc ttcatggttt cgggatgctc attatctccg gcaatcccgg tgatcggtgc
    17401 ttattatccc agctggtttt tctgcgccat tgccagcctt attttgatgc tcatcacgag
    17461 gcgagttatt cagcgggcaa atatcaatct ggcatttgtc ggaattattt ataccgccct
    17521 ttttgctctc tacgccatgc tgttctggct ggcatttttc taatctattt gagacgcgct
    17581 catggaaagt acgccgaaaa aagctcctcg cagtaaattc cctgctctgt tagtggttgc
    17641 attggcgctg gttgcccttg ttttcgttat ctggcgcgta gacagtgcgc catcaactaa
    17701 tgacgcttac gcgtcagcag ataccattga tgtggtgccg gaagtcagcg gccgcattgt
    17761 agaactggcg gtcaccgaca accaggcagt caaacagggc gatttgctgt tccgcattga
    17821 cccgcgcccg ttcgaagcca atctggcgaa agctgaagcc tccctcgcgg cgctggataa
    17881 gcaaattatg ctcacccagc gtagcgttga tgcgcaacag tttggtgccg actcgattaa
    17941 tgccacggta gaaaaagccc gtgccgccgc gaaacaggcc acagatacat tacgccgcac
    18001 cgagccatta ctgaaagagg gttttgtctc agcggaagac gttgaccgtg caagaactgc
    18061 gcagcgcgcc gcagaagccg atcttaatgc cgtattgtta caggcgcagt cagccgccag
    18121 cgccgtcagc ggcgtcgatg cgctggtggc gcaacgtgcg gcggtcgaag cggatattgc
    18181 cctgaccaaa cttcatctgg aaatggcgac cgttcgcgcg ccgtttgatg gccgggtcat
    18241 ttccctcaaa acctccgtcg ggcaatttgc ttctgccatg cgccctattt ttaccctaat
    18301 cgacactcgt cactggtacg tgatcgccaa cttccgcgaa accgatctga aaaatattcg
    18361 ctcaggtaca cccgcaacga ttcgcctgat gagtgacagc ggcaaaacct tcgagggtaa
    18421 agtggattcg attggctacg gcgtgctacc ggatgacggc ggcctggtgc tgggcggcct
    18481 gccgaaagtt tctcgctcta ttaactgggt ccgcgttgcc cagcgttttc cggtcaaaat
    18541 catggtcgat aaacctgacc cggaaatgtt ccgcatcggc gcttcggcag tcgctaatct
    18601 tgagccgcaa taatgagcgc gctcaactcc ctgccattac cggtggtcag gctgctggcg
    18661 ttctttcatg aagagttaag cgagcggcga cccggccgcg tgccgcagat cgtgcaactc
    18721 tgggtaggct gcctgctggt gattctgatc tcgatgactt ttgagatccc ttttgtggcg
    18781 ttatcgctgg cagtgctgtt ttacggtatt cagtcgaacg cgttttacac caaatttgtc
    18841 gcgatcttgt ttgtggttgc cacggtgctg gagatcggca gcctgttttt gatctacaaa
    18901 tggtcatacg gcgaaccgtt gatccgattg atcatcgccg ggccgatcct gatgggctgt
    18961 atgtttttga tgcgcaccca tcggctggga ctggtctttt tcgctgtcgc cattgtcgcc
    19021 atttacgggc aaaccttccc cgccatgctc gactatccgg aagtggtcgt gcgcttaacg
    19081 ctgtggtgta tcgttgttgg cctctatcca accttgctga tgacgttaat cggcgtgctg
    19141 tggtttccca gtcgtgccat tacgcaaatg catcaagcgc ttaatgatcg gcttgatgat
    19201 gccattagtc acctgacgga cagcctcgca ccgctacccg aaacgcggat tgaaagagag
    19261 gcgctggcgc tgcaaaaact gaatgtcttt tgtctcgcgg acgatgccaa ctggcgaact
    19321 caaagcgcat ggtggcaaag ctgcgtggca acggtaacct acatttactc gacgctgaat
    19381 cgctacgatc ccacctcttt tgctgattct caggcaatta ttgaatttcg gcaaaaatta
    19441 gcctcagaaa tcaataagct gcagcatgcc gttgctgaag gtcagtgctg gcaaagcgac
    19501 tggcggatca ctgaaagtga agcgatggcg gcacgggaat gtaacctgga gaatatctgc
    19561 cagacgttgt tacaactggg ccagatggac ccgaatacgc cgccaacgcc cgccgccaaa
    19621 ccgccatcaa tggtcgccga tgcttttacc aatccagact atatgcgcta cgcggtaaaa
    19681 acactgctcg cctgtttgat ctgttacacc ttctacagcg gcgtggactg ggaaggcatt
    19741 cacacctgta tgctgacatg cgtgatcgtc gctaacccaa atgtcggttc gtcgtaccag
    19801 aagatggtgc tgcgttttgg cggggccttt tgcggcgcga ttctggcgct gttattcacg
    19861 ctactggtca tgccctggct ggacaatatt gtcgaattgc tgtttgtgct ggcaccgatt
    19921 ttcctgttgg gcgcatggat tgccaccagc tctgaacgct cttcttatat cggcacacag
    19981 atggtggtca ccttcgcgct cgccacgctc gaaaacgttt ttggcccggt gtacgacctg
    20041 gtggaaattc gcgatcgcgc catgggtatc atcattggta ccgtggtgtc cgcggtgatt
    20101 tacacctttg tctggcctga aagtgaagcg cgcacactgc cgcaaaaact ggctggcacg
    20161 ctgggtatgt taagtaaagt aatgcggatc ccacgccagc aggaagtcac ggctctgcgc
    20221 acttatctgc aaattcgtat cggtctgcat gcggcgttta atgcctgtga agagatgtgc
    20281 caacgcgtgg cgctggagcg tcaactggac agcgaagaac gcgccttact gattgaacgt
    20341 tcgcaaacgg ttattcgtca gggccgcgat attcttcacg cctgggatgc cacctggaac
    20401 tcggcgcagg cgctggataa cgcactacag ccggacagag cagctcagtt tgccgacgcc
    20461 ctggagaaat acgctgccgg tctggcaacc gcactcagcc gttctcctca aataacgctt
    20521 gaagagacac ccgcctcgca ggccatcctg cccaccttat taaaacagga gcaacacgtc
    20581 tgccagcttt tcgcccgctt gccagactgg acagccccgg cattaacgcc cgccacggaa
    20641 caggcacaag gagccacgca atgatcaatc gtcaactttc acgtctgctg ttgtgcagca
    20701 ttctcggcag cacgacgctg atttccggct gtgccctggt acgtaaggat tctgcacctc
    20761 atcaacagct caaaccggaa caaatcaaac tggccgacga tattcatctt gccagctccg
    20821 gctggccgca ggcgcagtgg tggaaacaac tcaatgaccc gcagctggat gcgctgatcc
    20881 aacggacgct aagtggttca cacaccctcg ccgaagcgaa actgcgggaa gaaaaagcgc
    20941 agtcccaggc cgatttgtta gatgccggtt cacaattaca ggtcgcagcg ttagggatgc
    21001 tcaaccgcca acgcgtctcg gcgaacggct ttttaagccc ttatgcgatg gatgcgcccg
    21061 cactgggtat ggacgggccg tactatacgg aagccacagt aggtttgttt gccggactgg
    21121 atcttgattt gtggggtgtg catcgctcag cggttgccgc cgccattggc gcgcataatg
    21181 ccgcgctggc agaaaccgca gcagtagagc tatcgctgac cacgggcgta gcgcagcttt
    21241 attacagtat gcaggccagc tatcagatgc tcgatctgtt agaacaaact cgcgatgtga
    21301 ttgattacgc ggtgaaagcg caccagagta aagtggcgca cggtctggaa gcacaagtgc
    21361 ctttccacgg cgcgcgggca cagattctgg cggtcgataa acaaattgct gccgtcaaag
    21421 ggcaaatcac cgaaacgcga gaatctctgc gtgcattgat tggcgcgggg gccagcgata
    21481 tgccggagat caaaccggtg gcattaccac gagtccagac cggcattccg gcgacactct
    21541 cttatgagtt gctcgccaga cgcccggatc tgcaagccat gcgctggtat gttcaggcgt
    21601 cattagatca ggtggattcc gcgcgggcgc tgttctaccc gagctttgat atcaaagcgt
    21661 ttttcggcct tgatgccatc cacctggata ccttattcaa aaaaaccagt cgccagttca
    21721 acttcatccc gggtctgaaa ttgccgctgt ttgacggtgg acggttgaat gccaatctcg
    21781 aaggcacgcg cgccgccagc aacatgatga ttgaacgtta caaccagtca gtactgaacg
    21841 cggtgcgtga cgttgccgtc aacggcacgc gtctgcaaac gctcaacgac gagcgagaaa
    21901 tgcaggctga acgcgtggaa gcaacgcgct tcacccagcg cgctgccgag gccgcctatc
    21961 agcgcggctt aaccagccgc ttacaggcca ctgaagcccg gttgccagta cttgccgaag
    22021 agatgtcatt actgatgctg gacagccgcc gggtgatcca aagcattcag ttgatgaaat
    22081 cgctgggcgg cgggtatcag gcggctcccg tcgtcgagaa aaaataaaat gtctgccgcg
    22141 tgatggctgt cacgcggtat ttcgtttcgt cacgtcaaaa ctgacgacag cctgtttttc
    22201 gtcagagttt tgaataaata gtgcccgtaa tatcagggaa tgaccccaaa taaaatgtgg
    22261 cataaaagat gcatactgta gtcgagagcg cgtatgcgtg atttgattaa ctggagcgag
    22321 accgatgaaa aaagtcgtca cggtttgccc ctattgcgca tcaggttgca aaatcaacct
    22381 ggtcgtcgat aacggcaaaa tcgtccgggc ggaggcagcg caggggaaaa ccaaccaggg
    22441 taccctgtgt ctgaagggtt attatggctg ggatttcatt aacgataccc agatcctgac
    22501 cccgcgcctg aaaaccccca tgatccgtcg ccagcgtggc ggcaaactcg aacctgtttc
    22561 ctgggatgag gcactgaatt acgttgccga gcgcctgagc gccatcaaag agaagtacgg
    22621 tccggatgcc atccagacga ccggctcctc gcgtggtacg ggtaacgaaa ccaactatgt
    22681 aatgcaaaaa tttgcgcgcg ccgttattgg taccaataac gttgactgct gcgctcgtgt
    22741 ctgacacggc ccatcggttg caggtctgca ccaatcggtc ggtaatggcg caatgagcaa
    22801 tgctattaac gaaattgata ataccgattt agtgttcgtt ttcgggtaca acccggcgga
    22861 ttcccaccca atcgtggcga atcacgtaat taacgctaaa cgtaacgggg cgaaaattat
    22921 cgtctgcgat ccgcgcaaaa ttgaaaccgc gcgcattgct gacatgcaca ttgcactgaa
    22981 aaacggctcg aacatcgcgc tgttgaatgc gatgggccat gtcattattg aagaaaatct
    23041 gtacgacaaa gcgttcgtcg cttcccgtac agaaggcttt gaagagtatc gtaaaatcgt
    23101 tgaaggctac acgccggagt cggttgaaga tatcaccggc gtcagcgcca gtgagattcg
    23161 tcaggcggca cggatgtatg cccaggcgaa aagcgccgcc atcctgtggg gcatgggtgt
    23221 aacccagttc taccagggcg tggaaaccgt gcgttctctg accagcctcg cgatgctgac
    23281 cggtaacctc ggtaagccgc atgcgggtgt aaacccggtt cgtggtcaga acaacgtgca
    23341 gggtgcctgc gatatgggcg cgctgccgga tacgtatcct ggataccagt acgtgaaaga
    23401 tccggctaac cgcgagaaat tcgccaaagc ctggggcgtg gaaagcctgc ctgctcatac
    23461 cggttatcgc atcagcgagc tgccgcaccg cgcagcgcat ggcgaagtgc gtgccgcgta
    23521 cattatgggc gaagatccgc tgcaaactga cgcggagctg tcggcagtac gtaaagcctt
    23581 tgaagatctg gaactggtta tcgttcagga catctttatg accaaaaccg cgtcggcggc
    23641 ggatgtcatt ttgccgtcaa cgtcgtgggg cgagcatgaa ggcgtgttta ctgcggctga
    23701 ccgtggcttc cagcgtttct tcaaagcagt tgaaccgaaa tgggatctga aaacggactg
    23761 gcaaatcatc agtgaaatcg ccacccgtat gggttatccg atgcactaca acaacaccca
    23821 ggagatctgg gatgagttgc gtcatctgtg cccggatttc tacggtgcga cttacgagaa
    23881 aatgggcgaa ctgggcttca ttcagtggcc ttgccgcgat acttcagatg ccgatcaggg
    23941 gacttcttat ctgtttaaag agaagtttga taccccgaac ggtctggcac agttcttcac
    24001 ctgcgactgg gtagcgccaa tcgacaaact caccgacgag tacccgatgg tactgtcaac
    24061 ggtgcgtgaa gttggtcact actcttgccg ttcgatgacc ggtaactgtg cggcactggc
    24121 ggcgctggct gatgaacctg gctacgcaca aatcaatacc gaagacgcca aacgtctggg
    24181 tattgaagat gaggcattgg tttgggtgca ctcgcgtaaa ggcaaaatta tcacccgtgc
    24241 gcaggtcagc gatcgtccga acaaaggggc gatttacatg acctaccagt ggtggattgg
    24301 tgcctgtaac gagctggtta ccgaaaactt aagcccgatt acgaaaacgc cggaatacaa
    24361 atactgcgcc gtgcgcgtcg agccgatcgc cgatcagcgc gccgccgagc agtacgtgat
    24421 tgacgagtac aacaagttga aaactcgcct gcgcgaagcg gcactggcgt aatacagtcc
    24481 tttctacagc ctcctttcgg aggctgtttt tttatccatt cgaactcttt atactggtta
    24541 ctccctaccc aatcgtatta tcaaaatgaa aaaaattatc gcattgatgt tgtttttgac
    24601 attctttgcc cacgcgaacg actccgagcc tggcagccag tatttaaagg cagcagaggc
    24661 cggggaccga cgcgcacaat attttcttgc cgacagctgg tttagctccg gcgatttgag
    24721 caaagccgaa tattgggcac agaaagccgc cgacagcggt gatgctgatg cctgcgcgct
    24781 gctggcgcag atcaaaatca ccaatccggt cagtctggac tatccacaag caaaagttct
    24841 tgcagagaaa gcggcgcaag cgggcagtaa agaaggtgaa gtaacgctgg cgcatattct
    24901 ggtaaatact caagcgggta aaccggatta tccaaaggcg atttcgctgt tagaaaacgc
    24961 ctcggaagat ctggagaatg actccgccgt cgatgcccaa atgctgcttg gtttgattta
    25021 cgccaacggc gtgggcatta aggccgacga tgacaaggca acctggtatt tcaaacgcag
    25081 ctctgcaatt tcccgaaccg gttattccga gtactgggcg ggaatgatgt tcttaaacgg
    25141 tgaggaaggt tttatcgaga agaacaaaca aaaggcgctg cactggttga acctgagctg
    25201 tatggaaggg tttgataccg ggtgtgaaga gtttgaaaaa ttaaccaacg gttaaggtag
    25261 gcctgataag acgcaccagc gtcgcatcag gcatccacac attgccgggt ggataacccc
    25321 ggcaatcttc aattattgat ccgcagtttt atcaaatttg cccagcactt cacgctcata
    25381 agccagcgcc tttttgcggt caaatttgtg ttcccacttg gcaatcacca gcaccgccag
    25441 cgcattaccc accacgttca gcgcagtacg cgccatgtcg aggatacggt caacaccagc
    25501 aataaacgcc aggccttcca gcggaatacc tacgctaccc agcgttgcca gcagcaccac
    25561 aaacgacacg cccggcacgc cagcaatccc tttcgaggtc accatcagcg tcagcaccag
    25621 aatgatttcc tgccagatgg acaggtcaat gccatacagc tgagcgatga aaatagcggc
    25681 aatactttga tacagcgtcg aaccatcaag gttaaaagag taaccggtcg gcaccacgaa
    25741 gctggtgatc gacgccggtg ctccgtaggc ttccatcttc tcaataattc gcggcagtac
    25801 gctttcagag ctggcagtgg agtacgccag aatcagctca tctttcagaa tacgaatcag
    25861 gatccagacg cttaacccgc acaggcgcgc cacaattccc agcactacca gcgcgaagaa
    25921 cagaatggcg aaatgcacca gcagcaccag tttcgccagt ggccacagag aagagaaacc
    25981 aaagttagcc accgtcaccg caatcagcgc aaacacaccc accggtgcat aacgcatcac
    26041 catgtgagtc actttaaaca tggtttcaga gatggagcgg aacacggtca ccagcggttc
    26101 acgatgcgtc gcgggcaggg aggaaagccc cagaccaaac agcaccgaga agaagatgat
    26161 cggcagcatt tcgcctttcg ccatcgacgc cacaatgttc gtcggcacca gcgacaaaat
    26221 cgtgcccata atgccgtggg aactgctttg taccgcttcc gtagtgctct gatatttcga
    26281 gatatcgacg gtcgccagct gcgacatatc caccccggca ccgggctgga agacgttcgc
    26341 cagagtgatc cccaaaatga tggcgacggt ggtgatcacc tcgaagtaaa taatggtttt
    26401 cgcgccaata cgcccgagct gtttggcatc accaacaccc gcgataccca ccaccagcgt
    26461 ggagatcaca atcggcacaa caatcatttt gatcagatgg atgaagatat cacccgccgg
    26521 agagagcaaa ttgacgacca gccagtcgcg gctgtcgcta tggtagtgca ggtagcttcc
    26581 caggagaatg cccagcacca tagcaaacag aatctgccag gccaggctga attttatact
    26641 tttcatcaga ataacttcct caatggagcg ccgttccccg ttcagaaacg cgagaacgtg
    26701 acatactgcc agggcatgaa ttgtgttgcg ttggtttgta gattttttta acgcttatga
    26761 tcagtatcat ttgcgcggta tgttgcgcaa ccctttaaca acagagcttt tcgctgcatt
    26821 aatgcataat aacgcgagtt tatggcagga ttaatgaata accatttgtt atggaaagat
    26881 ttttatttgt ttaatctgca ataatactga ttggtgatta tttttggcat atgttcattc
    26941 gattattttt tatacaactc aataacataa tcttcatcac cagatgatta atctgatctc
    27001 cgctgcaaca atttcgccat attaatcgac tcgaccagct gtgtccggtt gatccggggt
    27061 gagttgagat ccatcacttt ttgccatagt tcgatggctt gcgcgtaatt cgcctgcata
    27121 aacgcatcgg aagccagcag catcagagcg gtgatttcgt tagagtccag cgcgagggct
    27181 ttgtcgatca ttgcgcgagt ctgggcggtc atatgctggc tggcctggta ataaagcacc
    27241 gtcgccagcg ccgcatacag ttcagcgttc tcaccacgta gttgcaacgc ctgacggtac
    27301 gccagcagcg aattgctgta atcgttttgc cacagatagt actcgcccag taacgcccat
    27361 tgttcgctgt tttgtggatt agcgcggatt ttatcctgca atgcctgaag ctgcgcttcc
    27421 ggggtttgct ggctggcaaa ctgatgcagc ggatcgcgct gacgctgata ttccgcacgt
    27481 accgcctgcc attttgggct taacagataa ctgccgacac agagaaatac catcaatatc
    27541 gtgagtgttg tcagcatttt caccggtact ttgggctgtt tcatcgcttc gccctcactc
    27601 gccagaggat cagtgccatc agcagtaaca acaccactgg cagcgcccat aacaccagcg
    27661 tctgacccgt taacggtggg ttatagcgaa caaaatctcc gtagcgttcg gtcatccagc
    27721 caatgatttc gacttcgttc ttcccctccg ccaccatgct gtaaacctga tggcgcatac
    27781 tgactgccac cggcgcgttg gattccagta agttttgatt ctggcactgc ggacaacgta
    27841 actggctggc aatatttaac gcctgttgct gttgttgtgg attggcgaat tgccaggtgt
    27901 ctacgacctg agcgcgcgca aaacaggtaa ataacagcag tagcgtgaga agccctttat
    27961 tcatcgcgct tcttcccccg ccatccgctt aacaatgcgc ccgcaatcat caacaatcct
    28021 cccccccaga tccagcgcac accgctttgt acatacaaac gaaaagcgta acgatccgct
    28081 ccggtttttt cacccatgac cgcataccag tcatggatgc cgttccagcg aattgacggt
    28141 tccatcattt gctgacgacg tgcttcgtaa aaacgccgct ccggcatcag ttcaccaatg
    28201 cgttgctgat ggtcaaacag tgccactatc gctttttcgc tggtgtaatt gcctttggct
    28261 tgcagatcga ggcgctcaaa acggaaggtg tatcctgcca gcgtcacctg ctgacccggc
    28321 tgtaaattca ggctgatctc ctgacggctg acgctggaaa ccacgatccc cgcggcaaat
    28381 aacagcacgc ccgcatgagc taccagcgcc ggaagctgca cgcgtttgcc agagacaaac
    28441 gtcgccagca caatcaccac cagcatcaac aggccaaacg gtaacgtcgc gcggttaaaa
    28501 tacggcgcgc caacggagag gcgtccccag cccagcagac cgtaaatcat cggataaagc
    28561 gttcccacca gcacgatcag tagcactgcg caaaacagca acagcgtagc gaggattaac
    28621 atttcccgcg ataaccccga aaaacgcacc accgcaccac cgtcccgcgc tcgccagcca
    28681 tacagagcca gagacgcaag gctaatcagt gcaaacaggc tgaacaacgg cacggcgcgg
    28741 acgttatcca gtgcgaacgc atgaaccgaa accagaatgc cagaacggac aattaaggta
    28801 cccagtagcg acagcatcag agttactatc gccagcaaca gcgaccagtg gcggaaaatc
    28861 tcccgctggc gtgtcaggga caaactgtgc agcagcgcag tggcagaaag ccagggtaat
    28921 aaagaggcgt tttccaccgg atcccagaac caccagccgc cccagcccag ttcgcaatag
    28981 gcccaccagg aaccgaggat gatccccgcc gttaatgcgc tccagccagg tagtgcccag
    29041 cgccaacaaa ttctggcgca ggcaccatca aactcgccgc gcagtaaact cgccagcgcc
    29101 acgctcgccg ctaccatcaa accgccatag ccaaggtaaa gcagcggtgg atgaaagata
    29161 agaccgggat gttgcagcat cggattgaga tcgcggcctt cgattgctgg tggaaatatc
    29221 cgcacgaagg gatcggacca cagcactaca aacagtagca gtgcggcgag catgagagat
    29281 aaaacggcta gcgtcagcgg aaatagcgga tcggtttgct gccgataatg ccaggcaaac
    29341 agcgcgctcc aggcggaaag cagcagcacc caaagcagca gcgaaccttc atggccgccc
    29401 cacaccgctg ccagcttgag ttcccacgac agcaggctgt agctatgttg ggcgacataa
    29461 atgaccgaga aatcgctgat gagaaaacaa tacgtcagta cgccaaaggc gagcaacagg
    29521 agcgcgaact gcgccagaat gccgatgcaa gtgagtcgca tcatggcagg ccagcgcaac
    29581 cgcactcccg caaaggccgt caacggggtc aacacgttga ccccgagact taacaacaac
    29641 gccagaaaac cgacttcagg aaggaatacg tccagaaacc acctcttttc tggcttacgc
    29701 caccgttaac tgcccggcgt agagaatgaa aaagcgcagc atcaacacgc cgaccagact
    29761 ggccccacac gccgccagca cggcaggaat gccggaactg cgattgaccc acggtttgag
    29821 caacattggc acaatcagcc ccagcccggc gacaccaagc cagaaccacc acgtccagaa
    29881 accgccacct aatgccgcca ccagcgcacg cactttaccg tcatcgccca gcgccagacc
    29941 gacaaaaaac gccaccagca ggaagatttc accccatacc acggggattt ccatacggtg
    30001 tacaaactgc gcttccgtgg aatgcgggtt actgcgttgg cgtatcgcca tggcgatcag
    30061 cgccaccgcc gcaccggacg agatgccgga gaagaggaac agcaccggca ggatcgggtt
    30121 attgaggaac ggatacgatt tcagcgccga cagcagaaag ccggtataag cccccaacaa
    30181 caccgccaac accagcatca atgtttccag tccgcgatga acgggcgtca gcaggctcag
    30241 aaccttttgc acgatcccca gcttcggcaa ccagcgttgt tgcagggcaa gcaaatcatg
    30301 ttcaaagatt ttcgccagcc acagcaccag caccaccatg tagagctgaa acagcatcac
    30361 ccccatcgac atcaccgagg taaaactgta gtggaacatc agcttccaga aggtccacgg
    30421 tcttgtcagg tggaagacga ggatcaacag accgaggatc accgcgcccg gcccgacaat
    30481 cagcgtggtg cgcagcaacg tactgtctgc accgcccgcc tgcgggtaga agcgacgtaa
    30541 cagcacggcc agcgtcacca gaccggcaga aataccaatc aaaaacaggt agatggcaat
    30601 cggccagtcc cacaccagcg attcaaaatg aaatgcggaa gtctgcgtca ttggctcacc
    30661 tccccgtatt taaacggtac gcggtacagc ttcggtttgg ttcccagcgc cagcttgtag
    30721 cggtaagtag gcttctggcg cagcagttgc gaaatctcac tgttaggatc gtccagattg
    30781 ccaaacgtca gcgccttcgt cgggcaagcc tcaacgcacg cgggcagctt accggcctgc
    30841 aaattggttt tacggcagaa atcgcatttg tccgccgtct tcgtgaccgg atggataaag
    30901 cgcacgcggt acggacaggc ggcgatgcag tactgacaac cgacgcagag atcaggatta
    30961 acatcgacaa tcccgctagc ggcatcacga aaagacgcac cggtcgggca gacgtcaacg
    31021 cacggcgcat gatcgcagtg ctggcaagac ttacggaaga agcgatattt cacatcagga
    31081 aattcgccct gcggctcgct acgaataatc gtcaggcgcg agacgccttc cggcaccttg
    31141 ttcacttccc gacaagcatc catacaggcg gtacagccga tgcataacga ctcgtcatgc
    31201 accatgccgt aacgcacccc attgatattc aacgttttcg ccaccacgcg ccctgcggtg
    31261 ccgctgacgg ctgccagcac gccgacgccg gtgagaaact ggcgacgaga ccaggtcatg
    31321 gctgctcctt aagcaacgga acagacgccg ggttaaagtt cggattggtg cgctgatcgc
    31381 tgtggcaatc gacgcaaatc ttaatccgtc ctttgtcact taacgtctgc atcgtatctt
    31441 gctgtggatg cagggaatgg cagctggcgc acgccacttt ggttacgtgg acatcgtgcg
    31501 gccagaacgc tttttgcaac tgttccggca gatgacagga catacagacg ctgttctgct
    31561 ctccaacctt gtacatcggc tcgttaaagc gcatcacatc tttcacccct tcgcggtgtt
    31621 gcggtgatgg ctggccgtga cagttggtgc aggtgaccgg cagtttgtta ttcgggttga
    31681 taacggaagc atgttttcca tgcatacctt cggtatctgg cttatgacag tccagacagg
    31741 cggcatccgg gttacgctgc tgggtaactt cgtagcgatc atcagatgcc tgcgccgcag
    31801 gggtagcggt aattccgttc aggctccaca acaggcccga cgccagcacc ccggcagtta
    31861 ataacgaacg taatacgctc atattcactc cattgtgttt tccgcccctt gcgaggcgga
    31921 acggggttat tggcttaaca gaccgttttt acgtgcctgt tcttcccact gcgggatcac
    31981 cgttttgatg aagtcctgct tctcggcctt gatttgttcc atgttcaggc caatagcctg
    32041 ctgggctttc tctttggttg agatatccgg gatctggatt tcatgggtga tgcctttggt
    32101 cgccagcagg cgcgccagtt tggtgcgtgc atccgccgct ttatccatcg ccgtaccgag
    32161 catccgtaaa ccttcttccg gtgcgtgcat atgaatgccg tgggaagcga tcgccagatc
    32221 ccagcgccac tgggcatgac ggatatcgtc ctgaattggc ttcatttcgg cttccgtcgc
    32281 gcctgcatcc agcgctgctt tcgcttcgaa gtgagcgtga accagttgat cttcaacctt
    32341 gattttcagg tcgttaatcg actgcttacg ttccgcgacc actttttgca aggcagcttt
    32401 gtcctgggta tggcagttcg cacaagtctg ggcgaagtta tcaaacggat taccaatttt
    32461 atggtcggtg tagagtttgc cttcggcgtt ctgcactttt ggcatatggc agtcgataca
    32521 ggtcacgttg tttttaccgt gaatgcccgc tgtccaggtt tcatattccg ggtgctgcgc
    32581 tttcagcatt ggcgttttcg acagggagtt agtccagtca gagaaggcaa ttttgtcgta
    32641 atactgctcc atattttcga ctttcatgcc gtcatcccac gggaatttaa ccgctttgtt
    32701 tttgccgtcg aagtaatact ccacatggca ctgaccgcaa accatcgatt gctggtcgaa
    32761 acgtccggct ttctcaaaag gtttaccaat ggcttccatc gcgcgagccg catacggacg
    32821 ggaaagggtt aactccggtt tgcctttggc gaactctggt gaggcggtgt tatggcagtc
    32881 ggcacaacct aagttgttga cgatttccgg gccgccgcgc gcccacttac cgtggaagta
    32941 gccatcttcg ccgtctttct ggatcagacg cgccacatcc gggcttttac aactccagca
    33001 cgccatcggt agcggaccat cttcagcgtt tttcggcgca ccggtacgca gggtttcacg
    33061 cacatcggtc acagcaaagg catgtccacg cggcttgttg taatcgcgcg agaagggata
    33121 ccccgcccac aggatcacca gccgtggatc ttccgccagg gcgtcaacac gctctgactg
    33181 ttccgaggtg gctttccagg agagatattg atcgggatgc tgcggggcaa aggtttcatt
    33241 cttcgcttcc acagttacag gttttgcggg agctgccgtt tgttcagcgt gaacagaagt
    33301 gaaaaagaaa aaaggaatca ataagctgaa gatacggcgt gcgtttattt ttatccttgt
    33361 cataggggct tcatccgaat tgcgccattg ttgcaatggc ggtttttatt gttttcacga
    33421 cagtaaccgc acctacactg tcatgacatt gctcgcccct atgtgtaaca aataaccaca
    33481 ctgtgaatgt tgtctttaat caattgtaag tgcatgtaaa ataccacttt agagttagtc
    33541 agtatcttcc tctttttcaa cagcatgcat aactgcatgt tcctcaaaga attaatcaac
    33601 ttttgttggt gaccttcaaa aattaccctg ccgtttattt gcacaattct acttttgcgt
    33661 gatctgtcgc ccaaatacta aacaaaactg ccaatacccc tacatttaac gcttatgcca
    33721 catattatta acatcctaca aggagaacaa aagcatgagc caaattcaca aacacaccat
    33781 tcctgccaac atcgcagacc gttgcctgat aaaccctcag cagtacgagg cgatgtatca
    33841 acaatctatt aacgtacctg ataccttctg gggcgaacag ggaaaaattc tcgactggat
    33901 caaaccgtac cagaaagtga aaaacacctc ctttgccccc ggtaatgtgt ccattaaatg
    33961 gtacgaggac ggcacgctga atctggcggc aaactgcctt gaccgccatc tgcaagaaaa
    34021 cggcgatcgc accgccatca tctgggaagg cgacgacgcc agccagagca aacatatcag
    34081 ctataaagag ctgcaccgcg acgtctgccg cttcgccaat accctgctcg agctgggcat
    34141 taaaaaaggt gatgtggtgg cgatttatat gccgatggtg ccggaagccg cggttgcgat
    34201 gctggcctgc gcccgcattg gcgcggtgca ttcggtgatt ttcggcggct tctcgccgga
    34261 agccgttgcc gggcgcatta ttgattccaa ctcacgactg gtgatcactt ccgacgaagg
    34321 tgtgcgtgcc gggcgcagta ttccgctgaa gaaaaacgtt gatgacgcgc tgaaaaaccc
    34381 gaacgtcacc agcgtagagc atgtggtggt actgaagcgt actggcggga aaattgactg
    34441 gcaggaaggg cgcgacctgt ggtggcacga cctggttgag caagcgagcg atcagcacca
    34501 ggcggaagag atgaacgccg aagatccgct gtttattctc tacacctccg gttctaccgg
    34561 taagccaaaa ggtgtgctgc atactaccgg cggttatctg gtgtacgcgg cgctgacctt
    34621 taaatatgtc tttgattatc atccgggtga tatctactgg tgcaccgccg atgtgggctg
    34681 ggtgaccgga cacagttact tgctgtacgg cccgctggcc tgcggtgcga ccacgctgat
    34741 gtttgaaggc gtacccaact ggccgacgcc tgcccgtatg gcgcaggtgg tggacaagca
    34801 tcaggtcaat attctctata ccgcacccac ggcgatccgc gcgctgatgg cggaaggcga
    34861 taaagcgatc gagggtaccg accgttcgtc gctgcgcatt ctcggttccg tgggcgagcc
    34921 aattaaccca gaagcgtggg agtggtactg gaaaaaaatc ggcaacgaga aatgtccggt
    34981 ggtcgatacc tggtggcaga ccgaaactgg cggtttcatg atcacgccgc tgcctggcgc
    35041 taccgagctg aaagccggtt cggcaacacg tccgttcttc ggcgtgcaac cggcgctggt
    35101 cgataacgaa ggtaacccgc tggaaggcgc taccgaaggc agcctggtga tcaccgactc
    35161 ctggccgggt caggcgcgta cgctgtttgg cgatcacgaa cgttttgaac agacctactt
    35221 ctccaccttc aaaaatatgt atttcagcgg cgacggcgcg cgtcgcgatg aagatggcta
    35281 ttactggatc accgggcgtg tggacgacgt gctgaacgtc tccggtcacc gtctggggac
    35341 ggcagagatt gagtcggcgc tggtggcgca tccgaagatt gccgaagccg ccgtagtagg
    35401 tattccgcac aatattaaag gtcaggcgat ctacgcctac gtcacgctta atcacgggga
    35461 ggaaccgtca ccagaactgt acgcagaagt ccgcaactgg gtgcgtaaag agattggccc
    35521 gctggcgacg ccagacgtgc tgcactggac cgactccctg cctaaaaccc gttccggcaa
    35581 aattatgcgc cgtattctgc gcaaaattgc ggcgggcgat accagcaacc tgggcgatac
    35641 ctcgacgctt gccgatcctg gcgttgtcga gaagctgctt gaagagaagc aggctatcgc
    35701 gatgccatcg taacccacaa ttgccggatg cgaatcggta acggtttgta ggcctgataa
    35761 gacgcgacag cgtcgcatca ggcattgatt gccggatgcg gcatataacg ccttatccgg
    35821 cctacattcg gcaagggtta cccgagcgtt aaccttctcc cataagggag cgggaattaa
    35881 aacaatccct acattacctc tggagaatct gtgatgaatg gcactattta tcagcggata
    35941 gaagacaatg cgcatttcag ggagttagtc gaaaaacggc aacggtttgc caccatcctg
    36001 tcgattatta tgctggcagt ttatatcggc tttattttac tgatcgcctt cgcgcccggc
    36061 tggctgggca cgccgctgaa tccgaacacc agcgtcacac gcggtattcc aattggtgtt
    36121 ggagtgattg tgatctcctt tgttctcacc ggtatctaca tctggcgggc gaacggcgaa
    36181 ttcgaccgtc ttaataatga agtcctgcat gaggtacaag catcatgaaa agagttctga
    36241 cggcgcttgc cgccacactc cctttcgcag ctaacgccgc ggatgctatt agcggggccg
    36301 tagagcgcca gccaacgaac tggcaggcga ttattatgtt cctgattttc gtcgtgttta
    36361 cgctcggcat tacctactgg gcatcaaaac gcgtacgttc tcgtagcgac tactacaccg
    36421 caggcggcaa tatcactggc ttccagaacg ggctggcgat tgccggggac tatatgtccg
    36481 ccgcctcatt cttggggatc tccgcgctgg tgtttacctc cggctatgac ggcttaattt
    36541 actcgctggg cttcctggtg ggctggccga tcattttgtt cctgattgcc gaacgtctgc
    36601 gtaacctggg gcgctacacc tttgccgatg tggcctctta ccgtctgaaa caagggccga
    36661 ttcgtattct ttcggcctgt ggttctctgg tggtggtggc gctttacctt atcgcccaga
    36721 tggtgggcgc aggtaaactg atcgagctgc tgtttggcct taactatcac attgcggtgg
    36781 tgctggtcgg cgtgctgatg atgatgtacg tcctgttcgg cggcatgctg gcgaccactt
    36841 gggtacagat tatcaaagcc gtgctgttgc tgttcggtgc cagctttatg gcctttatgg
    36901 tgatgaaaca cgtcggcttt agcttcaaca atctgttcag tgaagcgatg gcggtacacc
    36961 cgaaaggtgt cgacatcatg aagccgggcg ggctggtgaa agatccgatc tccgcgctct
    37021 ctctgggtct gggactgatg tttggtacgg cgggcttgcc gcacattctg atgcgcttct
    37081 ttacagtcag cgatgcccgc gaagcacgta agagcgtgtt ctacgccacc ggatttatgg
    37141 gctacttcta tattctgacc tttattatcg gcttcggcgc gatcatgctg gttggtgcga
    37201 atccggaata taaagacgcg gcgggccatc tgattggtgg taacaacatg gcggccgttc
    37261 acctggcgaa tgcagtgggc ggcaacctgt tcctcggttt tatttcagcg gttgcttttg
    37321 ccaccattct tgcggtggtt gctggtctga cgctggcggg cgcatcagcg gtttcgcatg
    37381 acctgtacgc taacgtcttc aaaaaaggcg cgaccgaacg tgaagaactg cgggtatcga
    37441 aaatcaccgt gctgatttta ggtgtgattg ccattatcct cggcgtgctg tttgagaatc
    37501 agaacatcgc cttcatggta ggcctggcgt ttgccatcgc agcgagctgt aacttcccga
    37561 tcattctgct ttctatgtac tggtcgaaac tgaccactcg tggcgcgatg atgggtggct
    37621 ggctggggct gattaccgca gtggtactga tgatcctcgg cccgacgatt tgggtacaga
    37681 tccttggtca cgaaaaagcc atcttcccgt atgaataccc ggcgctgttc tctatcaccg
    37741 tggcattcct cggcatctgg ttcttctcgg caaccgataa ctcagcggaa ggtgcgcgcg
    37801 agcgtgaact gttccgtgca cagtttatcc gctcccagac cggctttggc gttgagcaag
    37861 gccgcgcaca ttaatctctc cccttccccg gtcgcctgac cggggaatac tcttcctctc
    37921 caacatgcat caccttttcc cataatatta aacaaataaa ctcattaaaa aatgagcgat
    37981 ttttgacagt cgtagaaaat gataatgcag agaatatgcc ttttctttct tgttaattat
    38041 aaggatattt tatgcgctat aatggtttaa ataatatgtt tttccctctt tgcctgatta
    38101 acgataacca ctctgtcaca agtccatcac atacaaagaa aacaaaatca gataattaca
    38161 gcaaacatca taaaaacacg ttaattgaca ataaagccct ctctcttttc aagatggatg
    38221 atcatgaaaa agtgataggt ttgattcaga aaatgaaaag aatttatgat agtttaccat
    38281 caggaaaaat cacgaaggaa acggacagga aaatacataa acattttata gatatagctt
    38341 tatatgcaaa taataaatgt gacgatagaa ttacgagaag agtttacctt agtaaagaaa
    38401 aggaagtatc cattaaggtg gtatatttta taaataatgt cgccgtccat aataatacta
    38461 tcgaaattcc acagacagta aatggtggtt acgatttttc acaccttagc ctgaaaggta
    38521 tcgtgattaa agatgaagat ttatccaatt cgaattttgc aggttgcaga ctacaaaacg
    38581 ctattttcca ggactgtaat atgtataaaa cgaattttta ttacgccata atggaaaaaa
    38641 tactttttga tgattgtatt ctcgatgact caaatttcgc tcagataaaa atggccgacg
    38701 gaactctaaa tgcatgctcc gctatgcatg ttcaattcta caatgcagca atgaatagag
    38761 ccaatattaa aaacaccttc cttgactatt caaattttta tatggcgtac atggctgagg
    38821 taaatcttta taaagtaata gcgccatatg ttaatttatt taaagccgac cttagtttct
    38881 ctaaactcga tttaattaac tttgaacatg ctgatctgtc tcgcgtcaat ctgaacaaag
    38941 caatcctcca gaatataaac ttaattgata gcaaactctt ttgtacgtgg ctgacaaata
    39001 cattcctcga aatggttata tgtaccggct ctaatatggc taatgttaat tttaataatg
    39061 ccaatttaag caactgccat ttcaactgtt ctattttaac aaaagcctgt atgtttaata
    39121 cccgtctcta tcgggttaat tttgatgagg ctagcgtcca gggaatgggc atttccatac
    39181 tccgtgggga ggaaaatatc cccattgata gtgataccct ggtaacacga cagaaattct
    39241 ttgaagaaga ttgtacctct catactggca tgtcacaaac tgaggataat attaatgcag
    39301 tcgctatgaa gattactgca gatattatgc aacacgcaga ttgaggttaa atcgaagaac
    39361 agccaatgaa ttgactttac aagagagata acttatttgt tttagaatca tgaatgttag
    39421 cccgagctaa cctgttaaag gtgcaaaaga ttgcaccttt ttcagattac ctcgggcctg
    39481 gttacgtcgc tttcggctta ctgattttcc tcaattagca acgcttcaag caaatcgaga
    39541 tcgtgcagta atttttgcag cgtttcgttg ctgatctccc gcgtggcgcg caggtggtac
    39601 agttcagcac gttcagaacg caatgccgcc agacggaagc gacgctcaag gttctcttcc
    39661 tgcacggaac tttcaacgtc gttacgtcca tcggcgcgac gacgcaggtt accaatgacg
    39721 cgagaactga cctccgtaag cagctggtta tcgatattct cttcggtatc cgccgccaga
    39781 cgctcctcca ttttctggat cgccacaatc gccacttctg ccgttgccgc tcgcgcaata
    39841 cgttcttctt tcaattgttg cgaatggtcc gcgacttcaa tgtgttgtag cagaataggc
    39901 aacatcacca cgccgacaaa cagcgagaag agaatgacgc cagccgccag gaacaccagc
    39961 tcatagcgcg ccgggaagac gttaccatcc ggcaagagca gcgggattga gagcacaccg
    40021 gccagagtga tcgccccacg caccccggcg aaagacgcga tcaggatttc tcgtgtggtc
    40081 cacgaaccaa actccatcgg cttcttcttc aggaagcggt tgctgaactt tttcatcgtc
    40141 cacagccagc cgaaacggac cagcatcagc gccgcatata tcagaataat atcggtaaac
    40201 agcatccaga tttcgacgtt agggtcgatt tctgctgcca tcagcgacgt ctccagaata
    40261 cccggcagct gcagacctaa cagcaggaac accatgccgt taaagacaaa ttccagcatc
    40321 gcccaggtgc tgtttgcacg caggcgcatt gccagcggcg cacggcgcat cacaccggag
    40381 cgggtgatgg tcatcccggc ggcaaccgca gcgaggatgc cggaaacgcc aatatgttcg
    40441 gcaatcagat aagaagcgaa tggcagcaag aacagcagaa caatctgcgt cgcgggttca
    40501 tcaccgcccc agcggctgag gaatcgcagc gagcgaccat acagccagct caccacaaaa
    40561 ccggcaagaa taccgccaat ggctactttc ataaattcga ccgtcgcacc gccgacggtg
    40621 aagatcatcg tccccatcgc cactgccacg gcaaacttca acgacaccag gccggaagcg
    40681 tcattcatca acgcttcgcc ctgcaaaatg cccatgattt ttttcgggat gcgcccttcg
    40741 ccaacaatcc cggagagcgc cacagcatcg gtcggagaaa gtaccgccgc cagcgcaaag
    40801 gcggggatca gcggaatgcc cggcaccacc cagtaaataa ggaagccaat gccgaccacg
    40861 gtgaccacca ccagcgccag cgcgaggccg aaaatctctc gaccatgttc gagaaattca
    40921 cgggtcggcg ttttccagcc atcagcgaac agcaacggcg ggataaacaa gactaaaaag
    40981 agttcaggat caaactccac atgcaaacca aacgtcggcc acgccagtag cgcaccgatg
    41041 gcgatttgca taagcggaag cgggatctga aagggcatga cacgagtgac caccccggac
    41101 agcgagacca cgagggtcat tatcagtatg gtgaagaaga tttccatgcg ttccctgttt
    41161 gcgatgtttt tttatgatct gagaaagacg ttatgcctga attctcattt tttcagaata
    41221 tcgcaaaagc tcacacaatg tgcggagaaa ataaaaacgg gcagccattg ccacccgttt
    41281 tcagaatccg atgaaagatt agatagccca gccacccgca tagaaggtca ccagcgccac
    41341 ggcgataacg accgtaccga tgttcaactt gcgccattcg ccggaaacca gacgaccaat
    41401 caccagagtc gcgaagccga tcatgatgcc tgttacgatg ttacaggtca gcacgatgaa
    41461 taccgccgta accagacccg ccatcgcatc aacaaaatca gcaaagtcga ttttcgccac
    41521 gttgctcagc atcagcaggc caacgtacat cagcgccgga gccgttgcat accccggaac
    41581 gaggtaagag agcggagaga ggaacagaat cagcaggaac agcacgccaa cggtgatagc
    41641 cgtcaacccg gttttaccgc ccgccgccgt acccgccgca gactcgatgt ataccgctgc
    41701 cggagctgca cccaccaggc cagagaaaac gctgctcatg gagtcagtgg tcagtgcttt
    41761 cccaccgtcg ataatttgcc catctttatc cagcaggttc gcctggccgg cgacggcacg
    41821 gatagttccg gtggcatcaa atactgccgt cattaccagc gccagaacgc ttggcaggac
    41881 tacaggattc agcgcgccca taatgtccag gctgccaatc agtgagttgc cgttttcatc
    41941 gctcaatgaa ggcatggcga aaacgccgga gaaatggacg ttaggatcga agatcaaacc
    42001 gacaattgag ataccgataa tggtcagcag aatgccacca gggactttca gtttttccag
    42061 gccgatgatc accgccagac ctaccagtga cataatcacc gggaaggtcg cgaaatcacc
    42121 cagcgcaacg ggcagaccat caagcgggtt tttaatcacc agaccgacac cgttagcggc
    42181 aatgagcagc aggaacagac cgataccaat ccccgtgccg tgcgccacac cgtgcggcaa
    42241 gttgcgcaaa atccagctac ggatacccgt ggcagaaatt accgtaaaca gcacacccat
    42301 caggaacacg gcacccagcg cgacaggtac gctaatatgt tgccccagca ccaggctgaa
    42361 tgcggtaaac gccgtcaggg agatggcgca accaatcgcc aacggcagat tagcccacag
    42421 acccatcacg atagaaccga gtccggcaac cagacaggtt gcaacgaaaa ctgccgcagg
    42481 cgggaagcct gctttaccca acatacctgg aacgacgatg accgagtaga ccatcgccag
    42541 aaacgttgtt aacccggcaa ccacttcctg acggacagtg cttccacgtt gtgaaatttt
    42601 aaaccaggcg tcgagtgaac cgccggtacg cgctgatggc gtagacataa gaaaacatcc
    42661 cctgagagtt taattttcgt caagatgcgt ggcggacaat cccctgccag cgaaacgtta
    42721 tcttcctgct ccaggtttgc gacaatttac tgcgtcgcaa aaaagcaaac gtttagctcc
    42781 gcgcatacaa aacgggtggt aaaaaaagca aacgattatc cggcgatcat agcggctttt
    42841 tcaaccgaag tttgctgctt ttttctcttt tattgtcttg acgatgaaaa accaggcaat
    42901 tgatgcgcaa acgttatcgt cggtgcgcaa gattgcagcg ttatcgagcc gtataccgtt
    42961 caggattgcg ataaaaaatc gtctgagatg acaaaaatat tgcggataaa aagtatactt
    43021 ttacactggc gaagaaatgc cggtgacata caattaaagc atcaacacca accggaacct
    43081 ccaccacgtg ctcgaatggg gtgtgttgac gtcgggggaa accctcctgt gtaccagcgg
    43141 gatagagaga aagacaaaga ccggaaaaca aactaaagcg cccttgtggc gctttagttt
    43201 tgttcatctt ccagcaagcg tgcgccggta ccttcttctc ctaagcggtc gcccgggtta
    43261 cgcaacgggc aatcactgcg cgaaaggcag ccacaaccaa tacatccgtc cagttcgtca
    43321 cgcagcgcta ctaaggtatg aatgcgccga tccaactctt ctcgccattg ggacgaaagc
    43381 tgtttccact ctttcgcact taacgtatgc ccttcgggca acacgccaaa cgcttcacca
    43441 atggtcgcca gcggaatgcc aatacgctga gcaattttga taattgcaac atatcgcaac
    43501 acatcacgtt tatatcgccg ctgattgccg ctgttacgga tactggtaat caacccttta
    43561 ctttcataga aatgcagcgc cgataccgcc acaccgctgc gtttcgccac ttcgccgggg
    43621 gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac ctcaagttaa
    43681 cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa aagaggcaga
    43741 tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacga gcatattgac
    43801 cagccgctta acattgatgt agtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa
    43861 cgaatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca acgtcgcctg
    43921 ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat cgcaatggac
    43981 ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact
    44041 cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt
    44101 aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc
    44161 tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt ttcgacgcct
    44221 tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa
    44281 tgactggttt tgtgggtggt cagcgtttca acaaacgatt tgtcgatttt caaaatatcg
    44341 acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc atcaattgcc
    44401 acttcgtaac cagcctggcg gaaagccaga ataatcggcg tcattttttc gacatcaaga
    44461 aatgcatgct cagtcacttc aaatttaatt tgctgcggac gcaccgcgta ttgctctgtt
    44521 ttctgattga ttcgcgctat caaccgtgac gtatgaaaat cggaggccga caggttaata
    44581 gaaacatagc gatctgcatg tgttgccagg taatcgccca gatcgcggaa gacattatca
    44641 ataacataat cagttatctg ttctatcatc ccctcctttt ctgccagcgg aataaactct
    44701 gccggattca ttatttgccc ctgctcacca agccaacgta acaacgcttc agcgccgata
    44761 catttttctg ttttgatatc gattattggc tggtaataaa gacaaagttg atgtttttcg
    44821 agggcgcgtt gcaatttacg tttgggagat aaatagtttt gtcgaatacg tagccagagt
    44881 aatagcagaa caagactccc caaaataccg gcgggcaacg caaaaataag atgattataa
    44941 aaatgggtta taagacgttg atatgaagtc gcaacaatgg ctgcaattgg gcgttttgtc
    45001 gaataaactg tcgcatataa atagccattt ctttgtacgg ttaaatcctt caaatgaatc
    45061 agcggagaaa acgttgctgc cgaggcctct ttgcttaacg agaaaaaggt tttcgtcacc
    45121 gtatcataca caccccattg caatgtcgga tcatcagaca tcacttcact ccagaagaga
    45181 gggttgataa ccgccacata atttccccgc tgcatatagg tcattttata gccagagaaa
    45241 aaaggcgtat cgcggtaata atagatagaa acgttaggtt cacgcttata atcggccggt
    45301 gcaatcgtat agccgtttac aggcgctatc agcgatgagc ataaaaaatg gttatcacgg
    45361 gcatagatca attcattaat ataaagatag ccacgaataa tattcaacat tcgcttttga
    45421 tgggctggag tgcaaacttg cccctgatag cgttcagctg catcgctcac taaatctgcc
    45481 tggcgaatga ccagctcgga tttatccagt gccagttgag caaaggttcg gagctgatta
    45541 ttcacttctg attttgccca aaggaaggca atccatagcg aaagaatgat gggaaagaga
    45601 actaaaaaga taatgcccgg caacgccagt aattggtgtc gtgcacgatg actcattgct
    45661 atcaatttcc tgcctgttta tacccggcca ttgcaaggtg aaaaccctgg gtaccgacgt
    45721 ctttagataa atcttacggt ataaatagca cttttgcctg atttgaaatt gatacctttc
    45781 taatcgtcac ctggatgaaa ataattcatt gatattcaaa attaatttcc tacaggaatg
    45841 cagaggcggc gggaagatat gcccttaatg ccactgaaaa accacataat ctgtacagca
    45901 ataaaagtca cggcctaata tggctaacaa acaatcaatt gtgatatagt tcacaaaatt
    45961 aatgaaacaa acagagtgtt tcatttttgt gttctgcaag ccccgcgata gccagagtgt
    46021 accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt cagaggaatt
    46081 gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcaactt cttagtgccg
    46141 taatgatgtt tctggccagc acactcaaca tccgttttcg tcggtctgat tatgtcgggc
    46201 ttgcggtgat cagcagcggt ctgggcgttg tttccgcctg ttggttcgca atggggttgc
    46261 ttggcatcac aatggcggac atcaccgcta tctggcacaa catcgagttg gtgatgatag
    46321 aagagatgaa tcagacaccg ccacaatggc caatgatttt gacctgatac gtagaagcct
    46381 ccataacgga ggcttctttt ttatggctgg cagatgcttt aatcatccac cttaaaacaa
    46441 tataacctat tgttttaatg acaaatcaga acggaatgtc atcatcaaag tccatcggcg
    46501 gctcgttaga cggcgctgcc ggagcggact gctgcgggcg agactgcgcg ccgccgctga
    46561 actgattgcc accctgcggc tgctgaggct gaccccaacc gccctgcggc tgaccaccac
    46621 cgatattacc acctgccgga gcgccaccac cctgacgacc acccagcatc tgcatggtgc
    46681 cgccaacgtt caccacgact tctgtggtgt agcgatcctg accggattga tcggtccatt
    46741 tacgggtacg cagctgacct tcgatataaa cctgagaacc tttacgcaga tattcgctgg
    46801 ccacttctgc cagtttgccg aacagcacaa cgcggtgcca ttcagtctgt tctttcatct
    46861 cgccggtcgc tttatcacgc caggattcgg aagtagccag cgtaatgttg gcaactgcgc
    46921 caccatttgg catgtagcgt acttccgggt cctgacccag attaccaacg agaataacct
    46981 tgtttacgcc tctgctggcc atgttcgtgt ctcctgaaaa aaatcgttct gaataagtgt
    47041 aaacgcgcga ttgtaccatt accaatagcg cttttactat gttgtgacct cggttccggg
    47101 aaacaaacct ggccagacat tgttacacaa cactccgggt aatgcattcc aatactgtat
    47161 attcattcag gtcaatttgt gtcataatta accgtttgtg atcgccggta gcaccatgcc
    47221 accgggcaaa aaagcgttta atccgggaaa ggtgaatgga taagatcgaa gttcggggcg
    47281 cccgcaccca taatctcaaa aacatcaacc tcgttatccc ccgcgacaag ctcattgtcg
    47341 tgaccgggct ttcgggttct ggcaaatcct cgctcgcttt cgacacctta tatgccgaag
    47401 ggcagcgccg ttacgttgaa tccctttccg cctacgcgcg gcagtttctg tcactgatgg
    47461 aaaagccgga cgtcgatcat attgaggggc tttctcctgc catctcaatt gagcagaaat
    47521 cgacgtctca taacccgcgt tctacggtgg ggacaatcac cgaaatccac gactatttgc
    47581 gtttgttgtt cgcccgcgtc ggcgaaccgc gctgtccgga ccacgacgtc ccgctggcgg
    47641 cgcaaaccgt cagccagatg gtggataacg tgctgtcgca gccggaaggc aagcgtctga
    47701 tgctgctcgc gccaatcatt aaagagcgca aaggcgaaca caccaaaacg ctggagaacc
    47761 tggcaagcca gggttacatc cgtgctcgta ttgatggcga agtctgcgat ctttccgatc
    47821 cgccgaaact ggaactgcaa aagaaacata ccattgaagt ggtggttgat cgcttcaagg
    47881 tgcgtgacga tcttacccaa cgtcttgcgg agtcgtttga aaccgcgctg gagctttccg
    47941 gtggtacagc ggtagtggcg gatatggacg acccgaaagc ggaagagctg ctgttctccg
    48001 ccaacttcgc ctgcccaatt tgcggctaca gtatgcgtga actggagccg cgactgtttt
    48061 cgtttaacaa cccggcaggt gcctgcccaa cctgtgacgg ccttggcgta cagcaatatt
    48121 tcgatcctga ccgcgtgatc caaaaccccg agctgtcact ggctggcggt gcgatccgtg
    48181 gctgggatcg ccgcaacttc tattacttcc agatgctgaa atcgctggca gatcactata
    48241 agttcgacgt cgaagcgccg tggggcagcc tgagcgcgaa cgtgcataaa gtggtgttgt
    48301 acggttctgg caaagaaaac attgaattca aatacatgaa cgatcgtggc gatacctcca
    48361 tccgtcgtca tccgttcgaa ggcgtgctgc acaatatgga gcgccgttat aaagagacag
    48421 aatccagtgc ggtacgtgaa gaattagcca agtttatcag caatcgccca tgcgccagct
    48481 gcgaagggac gcgtctgcgt cgggaagcgc gccacgttta tgtcgagaat acgccgctgc
    48541 ctgctatctc cgacatgagc atcggtcatg cgatggaatt cttcaacaat ctcaaactcg
    48601 ctggtcaacg ggcgaagatt gcggaaaaaa tccttaaaga gatcggcgat cgtttgaaat
    48661 tcctcgttaa cgtcggcctg aattacctga cgctttcccg ctcggcagaa acgctttccg
    48721 gcggtgaagc ccagcgtatc cgtctggcga gccagattgg tgcgggcctg gttggcgtta
    48781 tgtacgtgct ggacgagcca tctatcggcc tgcaccagcg cgataacgag cgcctgttgg
    48841 gtacgcttat ccatctgcgc gatctcggta ataccgtgat tgtggtggag cacgacgaag
    48901 acgcaattcg cgccgctgac catgtgatcg acattggccc gggcgcaggt gttcacggcg
    48961 gtgaagtggt cgcagaaggt ccgctggaag cgattatggc ggtgccggag tcgttgaccg
    49021 ggcagtacat gagcggcaaa cgcaagattg aagtgccgaa gaaacgcgtt ccggcgaatc
    49081 cggaaaaagt gctgaagctg acaggcgcac gcggcaacaa cctgaaggac gtgacgctga
    49141 cgctgccggt gggtctgttt acctgcatca ccggggtttc aggttccggt aaatcgacgc
    49201 tgattaacga cacactgttc ccgattgccc aacgccagtt gaatggtgcg accatcgccg
    49261 aaccggcacc gtatcgcgat attcaggggc tggagcattt cgataaagtg atcgatatcg
    49321 accaaagccc aattggtcgt actccacgtt ctaacccggc gacctatacc ggcgtgttta
    49381 cacctgtgcg cgaactgttt gcgggcgtac cggaatcccg tgcgcgtggt tatacgccag
    49441 gacgtttcag ctttaacgtc cgtggcgggc gctgcgaagc ctgtcagggc gacggtgtga
    49501 tcaaagtgga gatgcacttc ctgccggata tctacgtgcc gtgcgatcag tgcaaaggta
    49561 aacgctataa ccgtgaaacg ctggaaatta agtacaaagg caaaaccatc cacgaagtgc
    49621 tggatatgac catcgaagag gcgcgtgagt tctttgatgc ggtgccagct ctggcgcgta
    49681 agctgcaaac gttgatggac gttggtctga cgtacattcg cctggggcag tccgcaacca
    49741 cactttctgg tggtgaagcc cagcgcgtga agctggcgcg tgagctgtca aaacgcggca
    49801 ccgggcagac gctgtatatt ctcgacgagc cgaccaccgg tctgcacttc gccgatattc
    49861 agcaactcct cgacgtgctg cataaactgc gcgatcaggg caataccatt gtggtgattg
    49921 agcacaatct cgacgtgatc aaaaccgctg actggattgt cgacctggga ccagaaggcg
    49981 gcagtggcgg cggcgaaatc ctcgtctccg gtacgccaga aaccgtcgcg gagtgcgaag
    50041 cttcgcatac ggcacgcttc ctcaagccga tgctgtaatc gttaaggccg ctttctgagc
    50101 ggccttttcc tttcagagtt gtaccagcaa tttacgcttt tcttccggta gtaaattcac
    50161 tgcctgctga taagacgcat ccaccagata atagatttgc gaatccggca gcgaaccgtc
    50221 gagatacacg gtgctccagt gcgctttatt cagatggcgg ctaggacgca catcgctgtg
    50281 ctgctgacgt agcagctccg ccagctccgg gctggttttc agcgaaacag ctgggcgatt
    50341 ttctacttct ttcaccatcg caaacagtac atcttccact ttgatctgcg tcgctttcca
    50401 gtcgttatgc acgctctgtt ctgcgcctgg ttttgccatg caatattgta gcaactccga
    50461 aatggtcatt ttttactccc cttgtagtgt cgcgatgatg cgacgcgatc cgccgtggat
    50521 gcgatgttcc cccagccaga tgccttgcca ggtgccggtc tgaatacgcc ctttatgtaa
    50581 cggcaataca agcgatgttc ccagcattga ggatttgata tgagaaggca tatcgtctgc
    50641 tccctcatag tcatgctcat aatttccgtt gtcgggaacg gtgcggagga aaaaacgctc
    50701 catgtcgtgg cgtacggtgg gatcgcagtt ctcattaagt gtcagagagg cggaggtatg
    50761 ttgcagcaac agatgcagta agccgatgtt aacgcgcggc atatcagcca gctgattcag
    50821 aatttcatcc gttaccagat gaaacccacg agatttggcg ctaagcgtga gcgtcttttg
    50881 ataccacatg tgctgctcct tgataaaact ctcttaatca gtttgcagca agacagcgaa
    50941 aggataaagg tgtgattaaa aaaacagcat tcgggagagc gtcacgctct cccgctctgt
    51001 cagtattctg aattgacgat cacctcttca ccaaacgcac ccgcttgtgg caagggtttg
    51061 taggtagagt tggaggcgcg cagaatgcgg ataccacgag cgccgacatc gcgtgcggcg
    51121 gtaatatcgt tatcggaatc gccataaaaa attcggatat ttttatcctg cagccattgc
    51181 gattttgtat tttgccctgg tttatcgccc gcaaagatca ccggattcat gttggtgaca
    51241 ggaatatgaa aattatccgc cagcgttttt gaaaccgttt ctgttttcgt cgggctacga
    51301 ccagtcacaa agaagatcgc gtcaccgcgg cgtacatgca tatcaatcag ctggcgagcg
    51361 acctcttttg gaatgctgaa ttcatcccag ccattgttca ttttttccca gaacacagga
    51421 tttttcagat aatcttcgct ttctggtgag aaggtttttt tgccgcgcca gaagcccggg
    51481 ctggaaaaaa gtaccgtgtc atcgatatca aaccccaccg ccattggcgg acgccctgcg
    51541 aggctatttt caatttgtgc gaccgaaacc caatgaattg gtgcctgttc agcaagcctg
    51601 gcaacgttag tcccagggtt aagcggtgaa ggagatgagg ccagggcaac agcggaactg
    51661 tttagagcga acaataagca aacggcactg attgcctgtg tgatcttgcg catatttttc
    51721 cctaaatagt cagtatgttg aaacttttta ttgtgagatt tgttgcaaaa accatctgac
    51781 cataacgcca gctgccgggg aaaggaagga ttatctgcgg tttttgtgat gaaaagcagg
    51841 atgattatgt ttaacccagt aaatacgttc ctgtcttttt ttgtataaaa aatgtttatt
    51901 actcgatgta aggatacatt aattttacgt ttattatgat gaatcctctg caaatgagcg
    51961 aaatttgata gaacatttat ctgctgtacg atttaatgaa cttaatgata cagcattaca
    52021 aaaacaatcg aagtttataa agatgattcc tgattgacca gaaccctatc ctacaatggt
    52081 gactaatttt attactcccg atggagatga tgatatgaat atatcatatg tgaatagcag
    52141 caaaacgaca tctttaccta ttgagctaga tgcactaaat aataaagata tctcttatgc
    52201 caaagatttt tcttatgcta aagatttctt tttatacata gaaacacagt taaaaattgc
    52261 aaaagatttt tgtagacctg gagaagaagt atcaagttct attgcaagta a
//
LOCUS       DAEVZA010000017        51860 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEVZA010000017 DAEVZA010000000
VERSION     DAEVZA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.017"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1218
                     /locus_tag="LAF92_001057"
     CDS             <1..1218
                     /locus_tag="LAF92_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HBI9790105.1"
                     /translation="QTGLPLWWGILVMAIGAISALLGVLYALAEQDIKRLLAWSTVEN
                     VGIILLAVGVAMVGLSLHDPLLTVVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTH
                     DMEKMGALAKRMPWTAAACLIGCLAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQL
                     AGPIAMVMLAVTGGLAVMCFVKMYGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALC
                     VLIALSASWLAPKIMHIAHAFTNTPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPL
                     LPGLYWLWCRSRRAAFRRTGDAWACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQL
                     DPTLRLNKGFAHVTARAQSTEPFWDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYV
                     VAALVVLLIAIAV"
     gene            1229..2176
                     /gene="hyfC"
                     /locus_tag="LAF92_001058"
     CDS             1229..2176
                     /gene="hyfC"
                     /locus_tag="LAF92_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBI9790106.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            2193..2303
                     /locus_tag="LAF92_001059"
     CDS             2193..2303
                     /locus_tag="LAF92_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HBI9790107.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            2315..3423
                     /locus_tag="LAF92_001060"
                     /pseudo
     CDS             2315..3423
                     /locus_tag="LAF92_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            3435..4085
                     /gene="hyfE"
                     /locus_tag="LAF92_001061"
     CDS             3435..4085
                     /gene="hyfE"
                     /locus_tag="LAF92_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBI9790108.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVINVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            4090..5670
                     /gene="hyfF"
                     /locus_tag="LAF92_001062"
     CDS             4090..5670
                     /gene="hyfF"
                     /locus_tag="LAF92_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBI9790109.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGNLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            5660..7375
                     /gene="hyfG"
                     /locus_tag="LAF92_001063"
     CDS             5660..7375
                     /gene="hyfG"
                     /locus_tag="LAF92_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HBI9790110.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRMMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            7385..7930
                     /gene="hyfH"
                     /locus_tag="LAF92_001064"
     CDS             7385..7930
                     /gene="hyfH"
                     /locus_tag="LAF92_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBI9790111.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFEPTVTN
                     KADLYTRATFHLQRCSRCERPFAQQKTVALAAELLAEQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            7927..8684
                     /gene="hyfI"
                     /locus_tag="LAF92_001065"
                     /pseudo
     CDS             7927..8684
                     /gene="hyfI"
                     /locus_tag="LAF92_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            8677..9090
                     /gene="hyfJ"
                     /locus_tag="LAF92_001066"
     CDS             8677..9090
                     /gene="hyfJ"
                     /locus_tag="LAF92_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBI9790112.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMNFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            9120..11132
                     /gene="hyfR"
                     /locus_tag="LAF92_001067"
     CDS             9120..11132
                     /gene="hyfR"
                     /locus_tag="LAF92_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBI9790113.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            11154..12002
                     /locus_tag="LAF92_001068"
     CDS             11154..12002
                     /locus_tag="LAF92_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HBI9790114.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHYIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(12040..13101)
                     /locus_tag="LAF92_001069"
     CDS             complement(12040..13101)
                     /locus_tag="LAF92_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI9790115.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            13314..14777
                     /gene="bepA"
                     /locus_tag="LAF92_001070"
     CDS             13314..14777
                     /gene="bepA"
                     /locus_tag="LAF92_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBI9790116.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            14798..15157
                     /gene="arsC"
                     /locus_tag="LAF92_001071"
     CDS             14798..15157
                     /gene="arsC"
                     /locus_tag="LAF92_001071"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBI9790117.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(15295..16041)
                     /gene="hda"
                     /locus_tag="LAF92_001072"
     CDS             complement(15295..16041)
                     /gene="hda"
                     /locus_tag="LAF92_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBI9790118.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(16091..17380)
                     /gene="uraA"
                     /locus_tag="LAF92_001073"
     CDS             complement(16091..17380)
                     /gene="uraA"
                     /locus_tag="LAF92_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBI9790119.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPATMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(17466..18092)
                     /gene="upp"
                     /locus_tag="LAF92_001074"
     CDS             complement(17466..18092)
                     /gene="upp"
                     /locus_tag="LAF92_001074"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBI9790120.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            18417..19454
                     /gene="purM"
                     /locus_tag="LAF92_001075"
     CDS             18417..19454
                     /gene="purM"
                     /locus_tag="LAF92_001075"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBI9790121.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            19454..20092
                     /gene="purN"
                     /locus_tag="LAF92_001076"
     CDS             19454..20092
                     /gene="purN"
                     /locus_tag="LAF92_001076"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBI9790122.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            20263..22329
                     /gene="ppk1"
                     /locus_tag="LAF92_001077"
     CDS             20263..22329
                     /gene="ppk1"
                     /locus_tag="LAF92_001077"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBI9790123.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            22334..23875
                     /gene="ppx"
                     /locus_tag="LAF92_001078"
     CDS             22334..23875
                     /gene="ppx"
                     /locus_tag="LAF92_001078"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBI9790124.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(23914..26157)
                     /gene="pdeF"
                     /locus_tag="LAF92_001079"
     CDS             complement(23914..26157)
                     /gene="pdeF"
                     /locus_tag="LAF92_001079"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBI9790125.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(26339..26491)
                     /locus_tag="LAF92_001080"
     CDS             complement(26339..26491)
                     /locus_tag="LAF92_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790126.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            26509..26700
                     /locus_tag="LAF92_001081"
     CDS             26509..26700
                     /locus_tag="LAF92_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBI9790127.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            27011..27529
                     /locus_tag="LAF92_001082"
     CDS             27011..27529
                     /locus_tag="LAF92_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBI9790128.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            27545..28084
                     /locus_tag="LAF92_001083"
     CDS             27545..28084
                     /locus_tag="LAF92_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBI9790129.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSESWFN"
     gene            complement(28177..29754)
                     /gene="guaA"
                     /locus_tag="LAF92_001084"
     CDS             complement(28177..29754)
                     /gene="guaA"
                     /locus_tag="LAF92_001084"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBI9790130.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(29823..31289)
                     /gene="guaB"
                     /locus_tag="LAF92_001085"
     CDS             complement(29823..31289)
                     /gene="guaB"
                     /locus_tag="LAF92_001085"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBI9790131.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            31451..32821
                     /gene="xseA"
                     /locus_tag="LAF92_001086"
     CDS             31451..32821
                     /gene="xseA"
                     /locus_tag="LAF92_001086"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBI9790132.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECYVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLVRQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(32818..33033)
                     /locus_tag="LAF92_001087"
     CDS             complement(32818..33033)
                     /locus_tag="LAF92_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBI9790133.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(33102..34574)
                     /gene="der"
                     /locus_tag="LAF92_001088"
     CDS             complement(33102..34574)
                     /gene="der"
                     /locus_tag="LAF92_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBI9790134.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(34692..35870)
                     /gene="bamB"
                     /locus_tag="LAF92_001089"
     CDS             complement(34692..35870)
                     /gene="bamB"
                     /locus_tag="LAF92_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBI9790135.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(35881..36501)
                     /gene="yfgM"
                     /locus_tag="LAF92_001090"
     CDS             complement(35881..36501)
                     /gene="yfgM"
                     /locus_tag="LAF92_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBI9790136.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(36519..37793)
                     /gene="hisS"
                     /locus_tag="LAF92_001091"
     CDS             complement(36519..37793)
                     /gene="hisS"
                     /locus_tag="LAF92_001091"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBI9790137.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQYKDKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(37904..39022)
                     /gene="ispG"
                     /locus_tag="LAF92_001092"
                     /gene_synonym="gcpE"
     CDS             complement(37904..39022)
                     /gene="ispG"
                     /locus_tag="LAF92_001092"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBI9790138.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(39049..40062)
                     /gene="rodZ"
                     /locus_tag="LAF92_001093"
     CDS             complement(39049..40062)
                     /gene="rodZ"
                     /locus_tag="LAF92_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBI9790139.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(40347..41501)
                     /gene="trmG/rlmN"
                     /locus_tag="LAF92_001094"
     CDS             complement(40347..41501)
                     /gene="trmG/rlmN"
                     /locus_tag="LAF92_001094"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBI9790140.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(41651..42082)
                     /gene="ndk"
                     /locus_tag="LAF92_001095"
     CDS             complement(41651..42082)
                     /gene="ndk"
                     /locus_tag="LAF92_001095"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBI9790141.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(42231..44543)
                     /gene="pbpC"
                     /locus_tag="LAF92_001096"
     CDS             complement(42231..44543)
                     /gene="pbpC"
                     /locus_tag="LAF92_001096"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBI9790142.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(44544..49505)
                     /gene="yfhM"
                     /locus_tag="LAF92_001097"
     CDS             complement(44544..49505)
                     /gene="yfhM"
                     /locus_tag="LAF92_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBI9790143.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPDETVADQQKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            49712..50557
                     /gene="sseA"
                     /locus_tag="LAF92_001098"
     CDS             49712..50557
                     /gene="sseA"
                     /locus_tag="LAF92_001098"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBI9790144.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(51050..51826)
                     /gene="sseB"
                     /locus_tag="LAF92_001099"
     CDS             complement(51050..51826)
                     /gene="sseB"
                     /locus_tag="LAF92_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBI9790145.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLGSTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
BASE COUNT        12334 a        13837 c        13183 g        12506 t
ORIGIN      
        1 caaacgggtt tgccgctgtg gtggggcatt ctggtgatgg cgatcggcgc aatctccgcg
       61 ctcctgggcg tgctgtatgc gctggcggaa caggatatca aacggctgct ggcctggagt
      121 accgtcgaaa acgtcggcat tattttgctg gcagtcggtg tggcgatggt cggtctgtca
      181 ctgcacgacc cgctgctcac cgtggttgga ctgctcggcg cactgtttca tctgctcaac
      241 catgcgctgt tcaaagggct gctatttctc ggcgcgggcg cgattatttc gcgtttgcat
      301 acccacgaca tggaaaaaat gggggcactg gcgaaacgga tgccgtggac agccgcagca
      361 tgcctgattg gttgcctcgc gatatcagcc attcctccgc tgaatggttt tatcagcgaa
      421 tggtacacct ggcagtcgct gttctcacta agtcgtgtgg aagccgtagc gctacaactt
      481 gcgggtccta ttgctatggt aatgctggca gtcactggtg ggctggcagt aatgtgcttc
      541 gtaaaaatgt acggtattac tttctgtggt gcgccgcgca gtacacacgc tgaagaggca
      601 caggaagtgc caaatacgat gatcgtcgcc atgctactgc tcgcggcact ctgcgtatta
      661 attgcgctta gtgccagttg gctggcaccg aagataatgc atattgccca tgcgtttacc
      721 aatacccctc ccgtcactgt cgccagcgga atagcacttg tacccggcac gtttcataca
      781 caggtcaccc cctcattact gttgctgtta ctactggcga tgcctttgct gcctggcctt
      841 tactggctgt ggtgtcgttc gcgccgcgca gcgtttcgtc gcacaggaga tgcctgggca
      901 tgcggctacg gctgggaaaa tgcgatggcc ccgtcaggca atggcgtgat gcagccgctg
      961 cgtgtggtct tctctgcgtt atttcgtcta cgacaacagc tcgaccctac gctgaggcta
     1021 aataaaggtt ttgcgcacgt caccgccagg gctcagagca cagaaccctt ctgggatgag
     1081 cgggtgatcc gccccatcgt gagcgccacc caacggctgg ccaaagaaat acagcatctg
     1141 caaagcggcg actttcgtct ctattgcctg tatgtggtcg ccgcactggt tgtgctgcta
     1201 atcgctattg ccgtctaagg aaatcaccat gagacaaact ctttgcgacg gatatctggt
     1261 tatttttgcg ttagcacagg ccgtgattct gctgatgcta accccacttt ttacgggtat
     1321 ttcccggcag atacgcgcgc gtatgcactc ccgccgcggg ccggggatct ggcaggatta
     1381 tcgcgatatc cacaaactgt ttaaacgcca ggaagttgcg ccgacatctt caggtctgat
     1441 gttccgcctg atgccgtggg tattaatcag cagcatgctg gtgctggcga tggccttacc
     1501 actgtttatt accgtttccc cttttgcggg cggcggcgat ctgatcaccc ttatctatct
     1561 tcttgccctg tttcgttttt tctttgctct ttccgggctg gataccggaa gtccgtttgc
     1621 gggagtcggt gccagtcgcg agttgacgct cggcattctg gtcgaaccaa tgcttattct
     1681 ctcactgctg gtattggcgc tgatagcagg ttccacgcat atcgagatga tcagcaatac
     1741 gctggcgatg ggctggaact cgccgctaac caccgtactg gcgttactgg cctgtggttt
     1801 tgcctgcttc attgagatgg gaaaaattcc ctttgatgtt gctgaagcag aacaggaatt
     1861 acaggaaggc ccgctgaccg aatattccgg tgccgggctg gcgctagcga aatgggggct
     1921 ggggctgaaa caggtcgtga tggcatcact gtttgtggcc ctgtttctgt cctttgggcg
     1981 cgcgcaagaa ctttctctcg cctgcctgct gacttcactt gtcgttacgc tgctcaaggt
     2041 tttgctgatt tttgtactgg cctcaatcgc ggaaaacacg ctggcacgcg ggcgtttttt
     2101 actcattcac catgtgacct ggcttggctt cagccttgct gcgcttgcat gggtcttctg
     2161 gttaaccggt ctgtaaggag cactgacgga atatggaaaa tcttgctctg acgacgttaa
     2221 tgctgccttt tatcggcgca ctggtcgttt cgttttcgcc acaacgtcgg gccgccgaat
     2281 gggggttttg ttcgccgcac tgaccacgct gtgcatgttg tcgctgatct ccgcgtttta
     2341 tcaggccgat aaagttgccg tcacgttgac gttggtcaac gtgggggatg tggcattgtt
     2401 tggcctggtc attgatcgcg tgagtacgct gattctgttt gtggtggtgt ttctcggttt
     2461 gctggtcacg atctactcca cgggttatct gacggataaa aatcgcgaac acccgcataa
     2521 cggcacgaat cgttattacg catttttgct ggtgtttatc ggcgcgatgg cgggactggt
     2581 actctcctcg acgctgctcg gtcagttgtt gttttttgaa attacgggcg gctgctcctg
     2641 ggcgttgatc agttattacc agagcgataa agcgcagcgt tcagcactaa aagcgttgct
     2701 tatcacccat atcggttcgt tggggttgta tcttgccgcc gccacgctgt ttttgcagac
     2761 cggaacgttt gtgcttagcg cgatgagcga gttacatggc gacgcacgtt atctggttta
     2821 tggcggcatc ctgtttgccg cgtgggggaa atcggcccag ctaccgatgc aagcgtggct
     2881 accggacgca atggaagcgc caacaccgat cagcgcctat ctccacgccg catcgatggt
     2941 gaaagtgggc ggctggcact ggagggcagc atcgcctaca tcgtcaacca cgcgttcgct
     3001 aaaagcctgt ttttccttgt agcaggtgcg ctgagttaca gctgcggcac gcgcttattg
     3061 ccgcgtctgc gtggcgtatt gcacaccctg ccgttgccag gcgtgggttt ctgcgtggca
     3121 gcgctggcga ttaccggtgt gccgccgttc aacggcttct tcagtaaatt cccgctgttt
     3181 gccgccggtt ttgcgttgtc agtggagtac tggatcctgc tgcccgccat gattctgctg
     3241 atgattgaat cggtcgccag tttcgcctgg tttattcgct ggtttggtcg cgttgtgcct
     3301 ggcaaaccga gcgaggccgt cgccgatgcc gcaccgctgc cagggtcaat gcgcctggtg
     3361 ttgattgtac tgattgtgat gtcgctgatt tccagcgtaa tcgccgcgac ctggttgcag
     3421 taaggagatg atgaatgacc ggttctatga tcgtaaataa tctggcggga ctgatgatgc
     3481 tgacatcgct gtttgtgatt aacgtcaaaa gctatcgcct gtcatgcgga ttttacgcct
     3541 gccagtcgct ggtgctggtg tctattttcg ccactctctc gtgcctgttc gccgcagagc
     3601 aactgctgat ctggtccgcc agcgccttta tcaccaaagt gctgctggta ccgttaatca
     3661 tgacttacgc tgcacgaaat attccccaga acatcccgga aaaagcgtta ttcggtccgg
     3721 caatgatggc actgctcgcg gcgttaattg tcctgctttg cgcatttgtc gttcagcccg
     3781 tgaagctacc gatggctacc gggctgaaac cggcgctggc ggtagcgtta ggtcattttc
     3841 tgcttggcct gctgtgcatt gtcagccagc gcaatatcct gcggcaaatt tttggttact
     3901 gcctgatgga aaacggctcc catctggtgc tggcgcttct tgcctggcga gcaccggaac
     3961 tggtggaaat aggtatcgct accgacgcca tcttcgccgt cattgtgatg gtgttactgg
     4021 caagaaaaat atggcgtacc cacggcacgc tggacgtgaa caacttgacc gcgctgaagg
     4081 gataatgaga tgagttattc tgtgatgttc gctttactcc tgctcacgcc gctgcttttt
     4141 tcgctgctct gttttgcctg ccggaaacgg gggctctctg cgacttgtac ggtgactgta
     4201 ttacatagct tagggatcac actgctgctg attctggcac tctgggtggt ccaaactgcc
     4261 gctgatgcag gagaaatatt cgctgcggga ctgtggcttc atattgatgg tctgggcggt
     4321 ttgttcctcg ccattcttgg tgtgattggc tttctcaccg gtgtttactc gattggctac
     4381 atgcgtcatg aagtggcaca cggcgagctt tcacccgtta cactgtgcga ttactacggt
     4441 ttcttccatc tgtttttgtt caccatgctg ctggttgtta ccagcaataa cctgattgtg
     4501 atgtgggcgg cgatcgaagc caccacctta agctcggcgt ttctggtagg catttacggt
     4561 cagcgttcat cgctggaatc agcatggaag tacatcatta tttgtactgt tggtgtcgct
     4621 tttggtctgt tcggtaccgt gctggtatac gccaacgccg ccagcgttat gccgcaggca
     4681 gaaatggcga tattctggag cgaggttctt aagcaatcgt ccttgcttga cccaacatta
     4741 atgctgttgg cctttgtgtt tgtgctaatt ggctttggta ccaaaaccgg gctatttccc
     4801 atgcacgcct ggctgccgga tgctcacagt gaagcgccga gtccggtcag cgccctgctc
     4861 tccgccgtat tgctgaactg cgcgctgttg gtgctgattc gctattacat cattatttgc
     4921 caagccatcg gcagcgattt ccccaaccgg ttgttgctca tcttcggcat gttgtcggtt
     4981 gccgtggcgg catttttcat tctggtacag cgggacatta agcgtctgct ggcgtactcc
     5041 agcgtggaga acatggggct ggtcgcggtg gcgctaggca ttggcgggcc gctgggaatt
     5101 tttgccgcgc tgctgcacat cttaaaccac agtctggcaa aaacgctgct gttctgcggt
     5161 tccggcaatg tactgctcaa gtacggcacg cgcgatctca acgtcgtctg tgggatgctc
     5221 aaaatcatgc catttaccgc cgtgctgttt ggcggcggtg cgctggcgct ggcagggatg
     5281 ccgcccttca acatttttct tagcgaattt atgaccatta ccgccggact ggcacgtaat
     5341 cacctgctga ttatcgtcct gctgttattg ctgttaacgc tggtgctggc gggcctggta
     5401 cggatggcag cgcgggtgtt aatggcgaaa ccgccgcagg ccgttaaccg gggtaatctc
     5461 ggctggttga ccacctcgcc aatggtgatt ctgctggtca tgatgctggc gatgggaacg
     5521 catattccac aacctgtcat caggatcctg gcgggcgctt ccactatagt cctctcaggg
     5581 acgcacgacc tgcctgcaca acgtagcacc tggcatgatt ttttgccttc aggcaccgca
     5641 tctgtttcgg agaaacacag tgaacgttaa ttcatcgtca aatcgtggcg aagcgattct
     5701 cgccgccctg aaaacgcagt tccccggcgc ggtgctggat gaagagcgac aaacgcctga
     5761 acaggtcacc attacggtga aaatcaatct gctgcctgac gttgtacatt atctttatta
     5821 tcaacatgat ggctggcttc cagtcctgtt tggcaacgac gagcggacac ttaacggtca
     5881 ttacgcggtt tattatgccc tttcaatgga aggggccgaa aaatgctgga tcgtggtgaa
     5941 ggcactggtc gatgccgaca gtcgggagtt tccgtcagtc acaccgcgcg tccctgccgc
     6001 ggtctggggc gagcgagaaa ttcgcgatat gtacgggctg attccggttg gcctgccgga
     6061 tcagcgtcgc ctggtgttgc ccgatgactg gccggaagat atgcatccgc tgcgcaaaga
     6121 tgcgatggat tatcgactgc gccctgaacc gacgacagat tccgaaacgt atgcgtttat
     6181 caatgagggc aacagcgatg cgcgggtgat ccctgtcggc ccgctgcata tcacctccga
     6241 tgaaccgggt cacttccgct tgtttgtgga tggcgagcaa attgtcgatg ctgattaccg
     6301 cctgttttat gtccatcgcg gcatggagaa actggcagaa acgcggatgg gctacaacga
     6361 agtgaccttc ttatcggacc gcgtgtgtgg gatttgcggt tttgcccaca gtgtggccta
     6421 taccaattcg gttgaaaatg cactggggat tgaggtgccg caacgagcac atactattcg
     6481 ctcgattctg ctggaagtcg aacggctaca cagtcatttg cttaaccttg gcctctcctg
     6541 ccatttcgtt ggttttgata ccggctttat gcaatttttc cgcgtgcggg aaaagtcgat
     6601 gacgatggcg gaattgctga tcgggtcgcg taaaacctac ggtctgaatc tgattggtgg
     6661 tgttcgccgc gatattctca aagagcaacg tctgcaaacg ctgaaactgg tgcgcgagat
     6721 gcgcgccgac gtgtcggagc tggtagagat gctgcttgcc acgccgaata tggaacaacg
     6781 cactcagggc attggcattc tcgaccgaca aatcgcccgt gattatagcc ctgtagggcc
     6841 gctgatccgc ggcagtggtt ttgcccgtga tttgcgcttt gatcacccct acgccgacta
     6901 cggcaatatt ccaaaaacac tgtttacctt taccggcggc gatgtcttct cccgcatgat
     6961 ggtccgtgtc aaagagacgt ttgattcgct ggcaatgctg gaatttgccc tcgacaacat
     7021 gccggatacc ccactgctga ccgaaggctt tagctataaa cctcacgcat tcgcgctggg
     7081 ctttgttgaa gcgccacgcg gcgaagacgt gcactggagc atgctcggtg ataaccaaaa
     7141 attgttccgc tggcgttgtc gtgccgccac ctacgccaac tggccggtgt tgcgttacat
     7201 gctgcgcggc aataccgttt ctgacgcacc gctgattatc ggtagccttg atccctgcta
     7261 ctcctgtacc gaccgtgtga cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgcc
     7321 gtataaagag atcgaacgct acggcattga tcgtaaccgt tcgccgctga agtaaggaca
     7381 gaagatgctg aagttactga aaactattat gcgcgccgga accgcgacgg tgaaatatcc
     7441 cttcgcgcca ctggaggtca gcccaggctt tcgcgggaaa ccggacctga tgcccagcca
     7501 atgtattgcc tgcggtgcct gcgcctgtgc ttgtccggca aatgcgctga ctatccagac
     7561 cgacgaccag caaaattcgc gcacctggca gctctatctg aggcgttgta tttactgcgg
     7621 acgttgtgaa gaagtgtgcc cgaccagagc catccagctt accaataact ttgaaccgac
     7681 cgtcaccaat aaagccgatc tctatacccg cgcgacgttc catctacaac gttgcagccg
     7741 ttgcgaacgc ccgtttgccc agcaaaaaac cgtcgcactg gctgctgaat tgttggcaga
     7801 gcaacaaaat gcgccacaaa accgcgaaat gttatgggcg caagcgagcg tctgcccgga
     7861 gtgcaaacaa cgcgcgacgc tgctcaacga cgatacagat gtaccgctgg tggctaagga
     7921 gcagctatga gtccagtgct tacacaacat gtcagccagc ccatcacgct ggacgagcaa
     7981 acgcaaaaga tgaagcagca tttgctacag gatatccgtc gctcggctta cgtttatcgc
     8041 gtcgattgcg gcggctgcaa cgcctgtgaa atcgaaattt ttgccgccat cacaccagta
     8101 ttcgacgcag aacgttttgg cattaaggtt atttcatcac cgcgtcacgc cgatattttg
     8161 ttatttacag gcgcagtcac ccgggcgatg cgtatgcctg cacttcgggc gtatgagtct
     8221 gcccccgatc ataaaatctg tgtttcctac ggcgcgtgcg gtgccggcgg cggattttcc
     8281 acgatctcta cagcgtctgg ggcggcagcg acaccattgt ccccattgat gtttggatcc
     8341 ccggctgccc gccaacaccg gccgccacca ttcacggttt cgccgtggcg ctcggtttgc
     8401 tgcaacagaa gattcacgcg gtggattatc gcgatcccac cggggtgact atgcaaccgt
     8461 tgtggccgca gatcccgcca tcacagcgta tcgccattga gcgagaagcg cggcggctgg
     8521 cgggctatcg tcaggggcga gaaatttgcg atcggctcct gcgccattta agcgacgatc
     8581 ctacaggaaa tcgggttaac acctggttgc gcgatgccga cgatccacgt ctcaatagta
     8641 tcgttcagca actctttcgc gtactccggg ggttacatga ctgaagagtg cggggaaatt
     8701 gttttctgga cgctgcgaaa aaagtttgtc gccagtagcg acgagatgcc ggaacacagc
     8761 tctcaggtaa tgtattactc gctggctatc ggtcatcacg ttggcgtgat tgattgtctg
     8821 aatgtcgcct tcccctgccc actgacggaa tacgaagatt ggcttgccca ggtcgaagag
     8881 gagcaagccc gacgtaagat gctgggggtg atgaattttg gtgagattgt tattgacgcc
     8941 agccacaccg ccctgttgac ccgggcattc gcgccactgg cggatgacgc gacgtctgtg
     9001 tggcaggcgc gtagcattca attcattcat ctgttggatg aaattgtgca ggaaccggcc
     9061 atctatctga tggccagaaa aattgcgtga gaaggatttc tcattaataa ggactgttga
     9121 tggctatgtc agacgaggcg atgtttgccc cgccacaagg aataacaatt gaagcggtaa
     9181 acggaatgct cgcggagcgg ttagcacaga aacacggtaa ggcgtcttta ttacgcgcct
     9241 tcatcccgat gccgccgccg ttcagcccgg tacaacttat tgaactgcat gttctcaaaa
     9301 gcaacttcta ttaccgctac catgatgatg gcagcgatgt gacggcaaca acagagtatc
     9361 agggcgagat ggtcgattat tcgcgtcacg ccgtccttct cggcagtagt ggaatggcgg
     9421 agctacgctt tattcgtacc cacggcagcc gttttactcc ccaggattgc acactgttta
     9481 actggctggc gcggataatc accccggttc tgcaatcatg gctcaatgat gaagaacagc
     9541 aggtggcgct gcgtttgctg gagaaagatc gcgatcatca tcgggtactg gttgatatta
     9601 ctaatgcagt gctgtcacat cttgatctcg acgatctgat cgctgacgtc gctcgtgaga
     9661 tccatcattt tttcggtctg gcttcagtca gtatggtact gggcgatcat cgaaagaacg
     9721 agaagtttag cctgtggtgc agcgatcttt ctgcctcaca ttgtgcgtgt ctgccacgca
     9781 atatgcctgg cgacagtgta ttgctgacac aaacgctaca aacccgacaa ccgaccttga
     9841 cgcaccgtgc agacgatctg tttctctggc aacgcgaccc gttattactc ttacttgcat
     9901 ctaacggctg cgaatctgcg ctccttatac cgcttacctt tggcaaccat acaccgggtg
     9961 cattgttgct ggcgcatacc tcttccactc tctttagtga ggaaaactgc cagctactac
    10021 aacacatagc cgatcgcatc gctattgccg ttggcaatgc cgatgcctgg cgtagcatga
    10081 ccgatttgca ggaaagtttg cagcaagaaa accaccagct tagcgagcag ctcctttcga
    10141 atctgggcat cggtgacatt atctatcaaa gccaggcaat ggaagaccta ctccagcagg
    10201 tagatattgt ggcgaagagc gacagtacgg tgttgatttg cggtgaaacc ggaaccggca
    10261 aagaggtgat cgccagagcg atccatcaac ttagcccgcg acgcgacaag ccgctggtca
    10321 aaatcaactg cgctgccatc cccgccagtc ttctggaaag tgagttattc ggtcatgaca
    10381 aaggggcgtt tactggtgcg attaataccc atcgtggtcg ttttgaaatt gccgatggcg
    10441 gcacgttgtt tctcgatgaa attggcgatc tgccgttaga acttcagcct aaactgctgc
    10501 gcgtattgca ggaacgggag attgagcgtc tcggcgggag tagaacgatc ccggtgaatg
    10561 tcagagtcat tgccgccacc aaccgtgatt tgtggcaaat ggttgaagat cgccagtttc
    10621 gcagcgatct cttttatcgc ctgaatgtct tcccactgga attgccgccg ctacgcgacc
    10681 gtccggaaga tatccctctt ttagcaaagc atttcacgca aaaaatggcg cgccatatga
    10741 atcgttcaat tgacgccatc ccgaccgagg cgctacgcca gttgatgtcg tgggactggc
    10801 cgggcaacgt gcgcgagctg gaaaacgtga ttgagcgagc ggtactgctg actcgcggta
    10861 acagtctgaa tttacatctt aatgtccgac aaagccgttt actgccgacg ctaaatgaag
    10921 attcagcgct tcgcagttca atggcgcagt tgctgcaccc gacgacgcca gagaatgacg
    10981 aagaagaacg tcagcgcatt gttcaggtat tgcgagaaac caatggcatt gttgccgggc
    11041 cccgtggcgc ggcgacacga ttagggatga agcgcaccac gctgctgtca cgaatgcagc
    11101 gtctggggat ctcggttcgc gaggtgttgt aatctgcttt tgcaggagta tgcatgagaa
    11161 acaaactctc tttcgacttg cagttgagcg ccagaaaagc ggcaatcgct gaacggattg
    11221 ccgcccataa aattgcccgc agtaaagtgt cggtcttttt aatggcgatg tccgctggcg
    11281 tgtttatggc gatcggattt actttttacc tttcagttat cgccgatgcc ccgtcttcac
    11341 aggcattaac ccatctggtg ggcggccttt gctttacact tggctttatt ttgctggcgg
    11401 tttgcggcac caacctgttc acctcgtcgg taatgacggt aatggcaaaa agtcggggcg
    11461 ttattagttg gcgaacttgg ctgattaacg cacttctggt ggcctgcggt aatctggcag
    11521 gtattgcctg tttcagtttg ttaatctggt tttccgggct ggtgatgagt gaaaacgcga
    11581 tgtggggagt cgcggtttta cactgcgccg agggcaaaat gcatcataca tttactgaat
    11641 ctgtcagcct cggcattatg tgcaatctga tggtttgcct ggcgctgtgg atgagttatt
    11701 gcgggcgttc gttatgcgac aaaatcgtcg ccatgatttt gcccatcacc ctgtttgtcg
    11761 ccagtggctt tgagcactat atcgccaatt tgtttgtgat tccgttcgcc attgccattc
    11821 gccatttcgc ccctacctcc ttctggcaac tggcgcacag tagcgcagac aattttccgg
    11881 cactgacggt cagccatttt attaccgcca atctgctccc ggtgatgctg ggtaatatta
    11941 tcggcggtgc agtgctggtg agtatttgtt atcgggctat ttatttacgt caggaatcct
    12001 gaaaaatcag cccggcgaaa cagttcgtcg ggctgaagat tattcttgcg cgatttgccc
    12061 atcgggccag gcgtggatca cggctttgat cagcgtcgcc aatggaatgg cgaagaatac
    12121 gccccagaat ccccacaaac caccgaagat caccaccgat aaaataatca ccagcggatg
    12181 caggttaacc gcttcggaga acaacaccgg tactagcagg ttgccgtcca gcgcctgaat
    12241 aatcagatac accgcgaagc agctccagaa ttccgtgcct gcgccaaact ggaatagcgc
    12301 cacgccaacc accggaatgg tcaccacaaa tgcgccaatg tacggaataa gaaccgagaa
    12361 gccgaccagc accgccagca gcagcgaata gttcagccca aagagcaaga accccagcca
    12421 ggtggcgatc cccaccacga tcatctccag cactttgccg cggatatagt tggtgatttg
    12481 ttgattcatc tccttccaca cctgtcctgc cagcccacgg ttacgcggca gcacccggcg
    12541 aacggcgttc agcatctgct ctttgtcttt cagcaggaag aagaccatca atggcaccag
    12601 caccagatag acggctatgg tcagcaaacc gaccagcgag gcgagggaaa ttttcaccac
    12661 cgaatcgccc atggtcagca tccgactgcg catattttcg gccattgcat caataatgcc
    12721 cgcgtccatt aacgccggat agcggcgcgg caacgtggcg gcaaagtcag aaagcttatt
    12781 gagcatcccc ggcatatcgc ggattaagta gatgccctgt tgccaggcga taggcagtac
    12841 cacgaacgcc atcagtagca atataccgac aaaaaccacc aatacaatcg acgtcgccca
    12901 gcggcgggag cagccaatag attgcaggcg cacggttggc cattccagca aataggccag
    12961 cacaatagcc accagcaacg gagcaagcag gccactaaag aaaaagataa tgccaaatcc
    13021 ggcaactaaa ataaccagca aggcaatcgc ttccgggtcg ctaaaacggc ggcgatacca
    13081 ttgcatcaac atttcgagca taaagacctt ccctgaacct caagagcggg attgcgatcc
    13141 gcaattgtat cgaaatgtca caaaaaagac ttcgcttttt atgacggatt caggaaactg
    13201 aaaagtcatt tgagtggact aatcttcgcc gttacactca aaggcggcgc ggtgggaacg
    13261 atatttcaca gtatcggtca aatgactatc tccagaaata caggatagag gttatgttca
    13321 ggcagttgaa aaaaaacctg gttgcaaccc tcattgctgc catgaccatt ggtcaggtag
    13381 ccccggcgtt tgccgatagt gcagacacct tgccggatat gggaacctcc gcaggaagca
    13441 cgctttccat tggtcaggaa atgcagatgg gcgactatta tgtccgccag ctacgcggca
    13501 gcgcgccgtt aattaatgat ccactattaa cgcagtacat taattcacta ggaatgcgtc
    13561 tggtttcgca tgccaattcg gttaagacac cgtttcattt ttttctgatc aacaacgacg
    13621 aaattaacgc ctttgctttc tttggcggca acgtggtact gcactctgcc ctgttccgtt
    13681 attccgataa cgaaagccaa ctggcttcag taatggcgca cgaaatctcc cacgtcaccc
    13741 aacgtcacct ggcgcgagcg atggaagatc aacagcgcag cgcgccgctg acctgggtcg
    13801 gcgcgttagg ttctatttta ctggcgatgg ccagtccgca ggcggggatg gcagcgctga
    13861 ccggtacact ggcgggaacg cgccagggga tgatcagctt cacccagcaa aatgaacagg
    13921 aagcggatcg cattggtatt caggtgctgc aacgctcggg attcgatccg caggcgatgc
    13981 caaccttcct cgaaaaatta ctcgatcagg cgcgttactc ctcgcgcccg ccggaaattt
    14041 tattgactca cccgttgccg gaaagtcgtc tggcagatgc ccgcaaccgt gctaatcaga
    14101 tgcgaccgat ggtggtgcag tcgtcggaag atttctatct ggcgaaagcg cgcacactgg
    14161 ggatgtataa ttccggacgt aaccagctca ccagtgattt gctggatgaa tgggcgaaag
    14221 gaaacgttcg tcagcaacga gcggcgcaat atggtcgtgc tttacaggcg atggaagcca
    14281 ataaatacga cgaggcgcgt aaaacgctgc aaccgttact ggcggcagaa cctggcaacg
    14341 catggtatct cgatctggct accgatatcg atcttgggca aaacaaagcc aatgaggcaa
    14401 tcaatcgcct gaaaaatgcc cgtgatttgc gcaccaatcc ggtgttgcag ctcaacctgg
    14461 cgaacgctta tctacaaggc ggtcaaccac aagaagcggc caatattctg aatcgctaca
    14521 cctttaataa taaagatgac agcaacggct gggatttact ggcacaggcg gaagccgcgc
    14581 taaataaccg cgatcaggag ctggctgcgc gagcagaagg ttatgcgctc gccggacgac
    14641 tcgatcaggc catttcgctg ttgagtagcg ccagttcgca ggtgaaatta ggcagcctgc
    14701 aacaagcgcg ttacgatgcg cgcatcgacc agttgcgcca gctgcaggaa cgctttaagc
    14761 cttataccaa gatgtaattc gcattaaagg aagaacaatg accaaacagg taaaaatcta
    14821 ccataaccca cgctgttcaa agagccggga aacgctgaac ctgctgaaag aaaacggcgt
    14881 ggagccagaa gtggtactct accttgagac acccgccgat gcggcaacgc tgcgcgattt
    14941 gctgaaaatg ctggggatga atagcgcccg tgaattgatg cgccagaaag aggatcttta
    15001 taaagaactg aacctggctg acagttcgct tagcgaagag gcgctgattc aggcaatggt
    15061 ggataatccg aagctaatgg agcgcccgat tgtggtcgcg aatggcaaag cgcggattgg
    15121 tcgtccaccg gagcaggtac tggaaatcgt gggttaagat gtgctccatc gctagttgaa
    15181 gcacattgcc ggatgcgacg cttgccacgt cttatccggc ctacgaatac tatggattaa
    15241 cttcgcgtag ttcggataag gcgttcgcgc cgcatccgat aataaacacc ttatctacaa
    15301 cttcagaatt tctttcacaa acggaatggt cagcttacgt tgcgcggtaa tcgacgcacg
    15361 atccaactga tccaacgtca taaatagcgt gcgcatttct ctgtcgagcc gcttcagcaa
    15421 gaaacgcccc acatcttccg gcagttcaaa accacgcaaa cgcgcgcgta actgtagcgc
    15481 ctgcaactta tcttcatcag aaagtggctg caatttgtag atctgccccc agtcgagtcg
    15541 cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt ggacgatcgc cggtgatcaa
    15601 caatcgtgtt ttgcccgatt ccagaattcg attgtagaga tcgaaaatcg ccatctccca
    15661 caactcatcg cctgcaatac actcaatgtt gtcgatacag accagcgaca aatgctccat
    15721 accgtcgagc acttccggaa caaaccaggt gcgtttatcc agcgggacat agcccaccgc
    15781 atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc agatggctgc gccccgcgcc
    15841 ttcgcgtgcc cagagataga tgtaaccgct atgttcctga cgcagcacgt tttgcagcgc
    15901 ggccagtaaa gaggagttat cccccggcca gaaacttgca aaggtttcgt cgtcaggaag
    15961 ataaagtggc aaagagagct gtgccggtgt gttcagagat acctcaacca ggatttcaca
    16021 aaatcgcgag aagtttacca cagaatccca tgatgtttga accgggcagc aacactgccc
    16081 ggtcggtaca ttatttgtct gttatatccg cgtcttctgc gtccagcacc acttcttcag
    16141 ggcgcagcac actgatcagt ttgaaaatca aacttaagcc gataccgacg atggtcgcca
    16201 gcgccatacc ttttaactcc gccgcgccga tgtttacctt cgcgccactg acgccgatga
    16261 tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt gtaatccact ttcgattcga
    16321 tcaaaacacg aataccggaa gcaccgatga caccataaag cagcagcgaa acgccgccca
    16381 tcaccggcaa tgggatcatc tggatagcgg cagccagttt accgacgcag gaaagcagga
    16441 tagcgaaaat cgccgccccg ccgataaccc aggtactgta aacacgggtg atcgccatca
    16501 cgccaatgtt ttctccgtaa gtggtatttg gcgtagagcc aaagaagccg gaaatcacgg
    16561 tcgacaagcc attagcaaac atcgaacggt gcagacctgg atcgcgcagc agatcttttt
    16621 tgacgatatt agccgttact accaggtgcc ctacgtgttc ggcaataacc actaacgccg
    16681 ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg cgtatagagg gttggcagcg
    16741 caaaccagtg agcattaata atcggcgtgg tatcgacaat tcccattgcg aaagagagcg
    16801 cgtaccccac cagcacgcca attaaaatcg ggataattgc caggaaacca cgaaacagca
    16861 cggaacctaa aaccgtgacc gccagggtgg taatagagat gatgatggtt ttggagtctg
    16921 gcgtttgccc ttcagccggg agtaaacccg ccataccggc agctacgccc gccagctcta
    16981 gaccgatgac ggcaacgatt gcgcccattg tcgcaggtgg aaacagcacg tccagccagc
    17041 cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa cagcacgccg cacataataa
    17101 agccgcccag cgcgacttca taccctaacg gcaacagtaa caataccggt gaaataaagg
    17161 caaagctgga accaagataa gccggaattt tccctttaca gatgaagaga tacagcagcg
    17221 ttccaatacc gttaaataac agtacagtcg ccgggttaat atgaaataag acgggcacca
    17281 ggacggttgc accaaacatg gcgaacaaat gttgcaaact aagcgggatt gtctgtaaaa
    17341 gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat agtattatcc tctgtattat
    17401 gtgttatagg cgctttactc aaaaaaaagc cgactcttaa agtcggcttt aattattttt
    17461 attctttatt tcgtaccaaa gattttgtca ccggcatcgc cgaggcccgg aataatgtat
    17521 ccgtgctcgt tcagtccctg gtcaatcgat gcggtataca gttcgacgtc cgggtgcgct
    17581 ttttccagcg cagcgatacc ttctggcgca gctaccagca ccagaacttt gatgctgctg
    17641 cagcccgctt ttttcagcag gtcgatggtc gcgataacgg aaccaccagt tgccagcatt
    17701 gggtcaacga tcagcgccat acgctcatcg atgttagaaa ccagtttctg gaagtacggt
    17761 accggctcca gcgtttcttc attacggtac ataccgacaa cgctgatgcg cgcgctcgga
    17821 acattttcca gcacaccgtc catcatcccc agaccggcac gcagaattgg cacaacggta
    17881 attttcttac ctttgatctg gtcgatttct accgggccgt tccagccttc gatagttact
    17941 ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca ggctacccac ttcggaagcg
    18001 agttcgcgaa agcgcttggt gctgatatct tgctcacgca tcagtcccag cttgtgtttg
    18061 acgagtgggt gtttgacttc cacgatcttc atactctttc tcctttgagg ggcagccaca
    18121 aaaaaaatcg acggattata cctcctttct tcaaggcggc aatattcttt tcgttgactt
    18181 tagtcaaaat gataacggtt tgagataaag ttattttata ttcaaatggt tatgaaagaa
    18241 gattattcca tccgaaaact aacctttgcc ctggcacaag tcttctttcg ccgcgcgtct
    18301 ggggaaaaga cgtgcaaaaa ggttgtgtaa agcagtctcg caaacgtttg ctttccctgt
    18361 tagaattgcg ccgaatttta tttttctacc gcaagtaacg cgtggggacc caagcagtga
    18421 ccgataaaac ctctcttagc tacaaagatg ccggtgttga tattgacgcg ggtaatgctc
    18481 tggttggaag aatcaaaggc gtagtgaaga aaacgcgtcg tccggaagtg atgggcggtc
    18541 tgggcggctt cggtgcgctg tgtgcattgc cgcaaaaata tcgtgaaccc gtgctggttt
    18601 ccggcactga cggcgtaggt accaagctgc gtctggcaat ggacttaaaa cgtcacgaca
    18661 ccattggtat tgatctggtc gccatgtgcg ttaatgacct ggtggtgcaa ggtgcagaac
    18721 cgctgttttt cctcgactat tacgcaaccg gaaaactgga tgttgatacc gcttcagcgg
    18781 tgatcagcgg cattgcggaa ggatgtctgc aatcgggctg ttcactggtg ggtggcgaaa
    18841 cggcagaaat gccggggatg tatcacggtg aagattacga tgtcgcgggt ttctgcgttg
    18901 gcgtggtaga aaaatcagaa atcatcgacg gctctaaagt cagcgacggc gatgtgctga
    18961 ttgcactcgg ttccagcggt ccgcactcga acggctattc gctggtgcgc aaaattcttg
    19021 aagtcagcgg ttgtgatccg caaaccaccg aacttgatgg taagccatta gccgatcatc
    19081 tgctggcacc gacccgcatt tacgtgaagt cagtgctgga gttgattgaa aaggtcgatg
    19141 tgcatgccat tgcgcacctg accggcggcg gcttctggga aaacattccg cgcgtattgc
    19201 cagataatac ccaggcagtg attgatgaat cttcctggca gtggccggaa gtgttcaact
    19261 ggctgcaaac ggcaggtaac gttgagcgcc atgaaatgta tcgcaccttc aactgcggcg
    19321 tcgggatgat tattgccctg cctgctccgg aagtggacaa agccctcgcc ctactcaatg
    19381 ccaacggtga aaacgcgtgg aaaatcggta tcatcaaagc ctctgattcc gaacaacgcg
    19441 tggttatcga ataatgaata ttgtggtgct tatttccggc aacggaagta atttacaggc
    19501 aattattgac gcctgtaaaa ccaacaaaat taaaggcacc gtacgggcag ttttcagcaa
    19561 taaggccgac gcgttcggcc ttgaacgcgc ccgccaggcg ggtattgcaa cgcatacgct
    19621 catcgccagc gcgtttgaca gtcgtgaggc ctatgaccgg gagttgattc atgaaatcga
    19681 catgtacgca cccgatgtgg tcgtgctggc tggttttatg cgcattctca gcccggcgtt
    19741 tgtctcccac tatgccgggc gtttgctgaa cattcaccct tctctgctgc cgaaatatcc
    19801 cggattacac acccatcgtc aggcgctgga aaatggtgat gaagagcacg gtacatcggt
    19861 gcatttcgtc accgatgaac tggacggtgg cccggttatt ttacaggcga aagtcccggt
    19921 atttgctggt gatacggaag atgacatcac cgcccgcgtg caaacccagg aacacgctat
    19981 ttatccactg gtgattagct ggtttgccga tggtcgtctg aaaatgcacg aaaacgccgc
    20041 gtggctggat ggtcaacgtc tgccgccgca gggctacgct gccgacgagt aatgccccgt
    20101 agttaaagcg ccagctctgc cgctggcgtt tttcaattca cctgtaaatc gcaagctcca
    20161 gcagtttttt tccccctttt ctggcatagt tggacatctg ccaatattgc tcgccataat
    20221 atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg taatgggtca ggaaaagcta
    20281 tacatcgaaa aagagctcag ttggttatcg ttcaatgaac gcgtgcttca ggaagcggcg
    20341 gacaaatcta acccgctgat tgaaaggatg cgtttcctgg ggatctattc caataacctt
    20401 gatgagttct ataaagtccg cttcgctgaa ctgaagcgac gcatcattat tagcgaagaa
    20461 caaggctcca actctcattc ccgccattta ctgggcaaaa ttcagtcccg ggtgctgaaa
    20521 gccgatcagg aattcgacgg cctctacaac gagctgctgc tggagatggc gcgtaaccag
    20581 atcttcctga ttaatgaacg ccagctctcc gtcaatcaac aaaactggct gcgtcattat
    20641 tttaagcagt atctgcgtca gcacattacg ccgattttaa tcaatcctga cactgactta
    20701 gtgcagttcc tgaaagatga ttacacctat ctggcggtgg aaattatccg tggcgatacc
    20761 atccgttacg cgctgctgga gatcccatca gataaagtgc cgcgctttgt gaatttaccg
    20821 ccagaagcgc cgcgtcgacg caagccgatg attcttctgg ataacattct gcgttactgc
    20881 cttgatgata ttttcaaagg cttctttgat tatgacgcgc tgaatgccta ttcaatgaag
    20941 atgacccgcg atgccgaata cgatttagtg catgagatgg aagccagcct gatggagttg
    21001 atgtcttcca gtctcaagca gcgtttaact gctgagccgg tgcgttttgt ttatcagcgc
    21061 gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac tgactatttc ccgctacgac
    21121 tccatcgtcc ccggcggtcg ttatcataat tttaaagact ttattaattt ccccaatgtc
    21181 ggcaaagcca atctggtgaa caaaccactg ccgcgtttac gccatatttg gtttgataaa
    21241 gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg atgtgttgct ctattatcct
    21301 tatcacacct ttgagcatgt gctggaactg ctgcgtcagg cttcgttcga cccgagcgta
    21361 ctggcgatta aaattaacat ttaccgcgtg gcgaaagatt cacgcatcat cgactcgatg
    21421 atccacgccg cacataacgg taagaaagtc accgtggtgg ttgagttaca ggcgcgtttc
    21481 gacgaagaag ctaacattca ctgggcgaag cgcctgaccg aagcaggcgt gcacgttatc
    21541 ttctctgcgc cggggctgaa aattcacgcc aaactgttcc tgatttcacg aaaagaaaac
    21601 ggtgaagtgg tgcgttacgc acacatcggg accgggaact ttaacgaaaa aaccgcgcgt
    21661 ctttatactg actattcgtt gctgaccgca gatgcgcgta tcaccaacga agtacggcgg
    21721 gtatttaact ttattgaaaa cccataccgc ccggtgacat ttgattattt aatggtgtca
    21781 ccgcaaaact ctcgccgtct gttatatgaa atggtagacc gcgaaatcgc caacgcgcag
    21841 caagggctgc ccagtggtat caccctgaag ctaaataacc ttgtcgataa aggactggtt
    21901 gatcgtctgt atgcggcctc cagctccggc gtaccggtta atctgctggt tcgcggaatg
    21961 tgttcgctga tccccaatct ggaaggcatt agcgacaaca ttcgtgccat cagtattgtt
    22021 gaccgttacc ttgaacatga ccgggtttat atttttgaaa atggcggcga taaaaaggtc
    22081 tacctttctt ccgccgactg gatgacgcgc aatattgatt atcgtattga agtggcgaca
    22141 ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca tcatcgacat attgttcagc
    22201 gatacggtca aagcacgtta tatcgataaa gaactcagta atcgctacgt tccccgcggc
    22261 aatcgccgca aagtacgggc gcagttggcg atttatgact acatcaaatc actcgaacaa
    22321 cctgaataac cctatgccaa tacacgataa atcccctcgt ccgcaggagt ttgctgcggt
    22381 cgatcttggt tcaaacagtt ttcacatggt catagcccgt gtggtagatg gtgccatgca
    22441 gattattggc cgcctgaaac agcgggtgca tctggcggac ggcctggggc cagataatat
    22501 gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg tcgctgtttg ccgaacggct
    22561 acaagggttt tctcctgcca gcgtctgtat agttggcacc catacactgc gtcaggcgct
    22621 gaacgccact gactttctga aacgcgcgga aaaggtcatt ccctacccga ttgaaattat
    22681 ttccggtaat gaagaagccc gtctgatttt tatgggcgtg gaacataccc aaccggaaaa
    22741 aggtcgcaaa ctggttattg atattggcgg cggatctacg gaactggtga ttggtgaaaa
    22801 tttcgaacct attctcgttg aaagccgccg gatggggtgt gtcagctttg cccagcttta
    22861 tttccctggc ggggtcatca ataaagagaa ttttcagcgc gctcgcatgg cggcagcaca
    22921 aaaactggaa actttaacct ggcaattccg tattcagggc tggaacgtgg caatgggcgc
    22981 ttccggtacc ataaaagccg cccatgaagt gttaatggaa atgggcgaga aagacgggat
    23041 aattaccccg gaacgtctgg aaaaactggt aaaagaagtt ttacgtcacc gtaatttcgc
    23101 atcgctgagt ttaccgggtc tttccgaaga gcggaaaaca gtcttcgttc ctggactggc
    23161 gattttatgc ggtgtgtttg atgctttagc catccgtgaa ctgcgccttt ctgacggggc
    23221 tttacgcgaa ggcgtactgt atgaaatgga aggacgtttc cgtcatcagg atgtgcgtag
    23281 tcgcaccgcc agcagcctcg ccaaccagta tcacatcgac agcgagcagg cccgacgggt
    23341 gctggatacc actatgcaaa tgtacgaaca gtggcgggaa cagcaaccga agctggcgca
    23401 tccgcaactg gaggcgctac tgcgatgggc cgccatgctg catgaggtcg ggttgaatat
    23461 caaccacagc ggtttgcatc gccactccgc ttatattctg caaaacagtg acttgccggg
    23521 ttttaatcag gaacagcagc tgatgatggc gacactggtg cgctatcacc gtaaagcgat
    23581 taagctcgac gatctgccgc gctttacctt gtttaagaag aaacagttcc tgccactgat
    23641 acagctattg cgccttggcg tattactaaa caatcaacgt caggcaacca ccacaccgcc
    23701 aacattgaca ctgattactg atgacagtca ctggacactg cgtttcccgc atgactggtt
    23761 tagtcagaat gcgctggtac tgcttgatct ggaaaaggag caagaatact gggaaggcgt
    23821 ggctggctgg cggttgaaaa ttgaagaaga aagtacacct gaaatcgccg cttaatgctt
    23881 tgcgggccga cgagaaatgt cggcccgcat tattcaggca ctttcgcgaa tgggttcgat
    23941 ttcattcagc gtatcaatta acggttgcgg cttaccaata agataaccct gcatataatc
    24001 gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact ctgccactac
    24061 ccgcattttc ttcattcgcg ccaggtggca aatcgatgcc actatctgat aatccagact
    24121 atttgacaca atattgcgga taaaactgcc gtcaatttta agcagatcgg cattcacatt
    24181 tttaagccgc gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
    24241 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
    24301 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca gcaactgact
    24361 gacttcaaca ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
    24421 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
    24481 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc tatcggggcc
    24541 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
    24601 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
    24661 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg ccccgcgacg
    24721 ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat ttagctctcc
    24781 cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga cgccaatctg
    24841 cggttgcatc ggcatgccat cccaaaagaa acgaaattgc ttgagatggc tatccagtgc
    24901 ggtaatgcgc tcctggtgcg attctgtatt cagccgcagc gcgagatcgt tacccgaaag
    24961 ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa gtttttgctt
    25021 gtattgaatc cgcagcatga tgccatagtt cttaaccagc atttccatgc cagggatgcg
    25081 taaataacaa agcgcagacc agggggcatc acgcaacgcg cgattcaggg cgcgaacatt
    25141 tggcagatga accaccgggt ccacatacgc aagccgctga atgcgtctga ctaccgctcg
    25201 ctgacgagtt gccagtacag ccatataatt gacaataaaa gagaatacca gataactgga
    25261 ggaggttatg gtcagctgcg tggtatagcc aggataaatg ggaatgtaat tttgatagct
    25321 gtggatgctg atcatcaaca cgaccgccca gagcagcgaa atcagcttat aaccatagcg
    25381 catcgctccc cacatcatca ggggcagcaa taatgacaag gtataattgg tgctaaaaat
    25441 tgtgcttttt tcatttaacg gcatgcataa cagcaacaat aaagcaccta atgccagtag
    25501 ccagagcgcg aactcttttt tggtgacttt ggcatcaacc tgctgtttta attgcgaata
    25561 atagctacgt aaataaaatg gatttcgcac tacccgaatg atgaagtagc acagcgggac
    25621 accgatcaga ttacccacca gtaaggcctg ataattgatt aaagtcccga ggttaaaagg
    25681 catgacaccg accagatttt ctctgctcgc cagcaatcct acaaatgcag caaactggaa
    25741 aagtatcaga aacagcgttg caggaaacac tatctgccag aatatacgct gggaaattaa
    25801 acgggtatca ccatgtgaaa cgttgttacg ccggggagta aagaccctgt aaccgcccca
    25861 acagagaatg ataatgataa taaaattagc cgttaatgag agcgtttcgt aaaaacccag
    25921 ctctgcatat ttgcggacaa aaatccctaa ggtgatccct ggcaatgccg cccagctgaa
    25981 aaacatcatc atgctgatca tcagtgccaa aggcagataa aagagaaaaa cctcaccgga
    26041 agaaatatgc gcgaaagtat taatgtgggc gaaaatgggc aagattaaag aaggcaggaa
    26101 cagcggaagc ccccaccatt tatcacgtat ttttatataa gttgcattca gtttcataga
    26161 tgctcagcag aatcccccac atcctgaagg aggtgtattc agacaggcat cccacctgac
    26221 ttcgaatgat gattattcat cactatagag agcattgatt ctaagtgtca tatgaaagtt
    26281 ccaattgata tatatcaaac aaaataaccc tgattaatga attattacgt ttatcatgtt
    26341 aattcatcat tattacatca tcattgtaaa taattaaatt aacttccata acattaaaat
    26401 atgtatccac tgacgctttt ttacataacg aagaattgac cattttgtcc tgttgtgcct
    26461 taatgtaagt accgtccaca gcgtgggaca tacttcaagg aaccttttgt gagtcaggca
    26521 accagtatgc gaaaacgaca ccgatttaac agtcgcatga cccgtatcgt actgctcatc
    26581 agctttatct tcttctttgg ccgttttatc tactcgtccg tcggtgcctg gcagcaccat
    26641 cagagcaaaa aagaagctca gcaatccaca ctctccgtcg aatcaccggt acaacgttag
    26701 cggttacctt ctccactttc acagaaccta acggcacttc gctgtcggat gcttttgctc
    26761 tttggaatta tcaaagcggc agatattctt tcatcttaaa ttttacgtct ttgtcctgac
    26821 tgatgtttat cctgtttggg tgcgaaataa atataaaatt aatatatatg ttgtaatgat
    26881 atatttttat aaattattcc ctgcgtgaat tttaataaat ttaatctatc cctttatacg
    26941 caatacattt actttcctct tttgatgatc ttaaatgtct tatttttcgt aatgtgtata
    27001 acaaggaata gtgatgaaat ttaaaaaatg tcttctgcct gtggcaatgt tagcgtcatt
    27061 cactctggca ggatgccagt caaatgctga cgatcatgct gccgatgttt atcaaaccga
    27121 tcaactgaat accaaacaag aaactaaaac cgttaatatt atttccattc ttcccgcaaa
    27181 agttgccgta gacaactccc aaaataaacg gaacgcacaa gccttcggcg cgcttattgg
    27241 cgcagtcgct ggcggtgtta tcggccacaa cgtcgggtct ggcagcaatt ccggaacgac
    27301 ggcaggtgca gttggcggcg gagctgtagg cgcggcagcg ggttctatgg tgaatgataa
    27361 aaccttagtg gaaggtgttt ctttaaccta taaggaaggc accaaagtgt atacctctac
    27421 ccaggtgggt aaagagtgcc agtttacgac aggtttagcc gttgttatta ccacgacgta
    27481 taacgaaacg cgtattcagc caaataccaa atgtcctgaa aagagctaat aatcaggagg
    27541 agtcatgaag aaagtttttc tttgcgccat cttagcctcc ttaagctatc cggctatcgc
    27601 ctcatcattg caggatcaac tctctgctgt cgcagaagcg gaacagcaag gtaaaaatga
    27661 agagcaaagg cagcatgacg aatgggtcgc ggagcgcaac agggaaatcc agcaagagaa
    27721 gcaacgtcgc gcaaatgccc aggccgccgc taacaaaaga gcggcaacgg cagcggcaaa
    27781 taagaaagct cgtcaggata aactggacgc cgaagccact gcggacaaaa aacgcgatca
    27841 aagttatgaa gatgagctac gcagcttaga gattcagaaa caaaaactgg cgctggcgaa
    27901 agaagaagcc cgcgttaagc gcgaaaacga atttatcgat caggaactga agcacaaagc
    27961 tgcgcaaacc gatgtggtgc aatctgaagc tgacgccaac agaaatatga ctgaaggcgg
    28021 tcgcgatctg atgaaaagcg tgggcaaagc agaagagaac aaatcggaaa gctggtttaa
    28081 ttaatcgatg ttagtaactt caagcctatg attcttgaag ataaaaaacc ctctgtagta
    28141 acagagggtt ttgttcattc atagtgcagg gtcaaatcat tcccactcaa tggtagctgg
    28201 cggcttgccg ctgatgtcat acaccacgcg agaaataccg ttcacttcat tgataatgcg
    28261 gttggaaaca cgaccgagga aatcgtacgg cagatgcgcc cagtgtgcgg tcataaagtc
    28321 gatggtttcg acagcacgca gagagacaac ccagtcatac ttacgaccat cgcccattac
    28381 gccaacggaa cgtaccggca ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc
    28441 cgctttacgc agttcttcaa tgaagatggc gtcagcacgg cgcagcaggt cacagtactc
    28501 tttcttcact tcacccagca cacgaacgcc aaggcctggt cccgggaacg ggtgacggta
    28561 cagcatgtcg tacggcaggc ccagctccag accaatctta cgcacttcgt ctttgaacag
    28621 ctctttcagc ggttcaacca ggcccatctt catctctttc ggcaggccgc ccacgttgtg
    28681 gtgagatttg atgacgtgtg ctttaccggt tgcagaagcc gcagattcga taacgtcagg
    28741 gtagatggtg ccctgcgcca gccacttcac gtcttccagt ttcagcgctt cttcatcgaa
    28801 cacttcaacg aaaacgcgcc cgatgatttt acgttttgct tccggatcgt tttcgccagc
    28861 cagcgctgac aggaagcgat cttctgccgg tacgtgaaca atgttcagac caaagtgatc
    28921 gccaaacata tccagaacct gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa
    28981 tacgcaagtc aggtttttac cgatagcgcg gtgcagcagc attgcggtta cggaggaatc
    29041 cacaccacca gagaggccga ggatgacttt atcgtcgcct acctgctcgc ggatgcgagc
    29101 tacagcatcg tcgataattt tcgctggcgt ccacagggct tcacactggc agatatcacg
    29161 cacaaaacgc tccagcatgc gcataccctg gcgggtgtga gtcacttccg ggtggaactg
    29221 tacgccatag aagcgttttt cttcgttagc cataatggca aacgggcagc tttcggtgct
    29281 ggctacggtg acgaagtcgg acgggatagc ggtaacttta tcgccgtggc tcatccagac
    29341 atcgagcagc ggtttaccgt ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcgct
    29401 gtcgtttacg acttcaacct gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg
    29461 accgcccaac tgcattgcca tggtctgcat gccatagcaa acgccgaata ccggtacgcc
    29521 tgcttcaaag acatactgcg gcgcacgcgg gctgttttct tcggtggtgc tttccgggcc
    29581 gccggaaaga ataatgccgc ttggattgaa gtcacgaatt tgtgcttctg tcacatccca
    29641 cgcccacagt tcgcagtaaa cacccagctc acgcacgcgg cgcgcaacca gttgagtgta
    29701 ctgagaaccg aagtccagaa tgaggatgcg atgcttatga atgttttccg tcattgacgc
    29761 ttattccgag gcaagtgaaa cagataatat aaatcgcccg acatgaagtc gggcgaagag
    29821 aatcaggagc ccagacggta gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg
    29881 ctttcctgaa tgcccgcacc gctgatacgt acaaactccg ctttagtacg cagttcgtcg
    29941 atagtaccac agccggtcag acccatacag gagcgcaggc cgcccatctg ctggtgaatg
    30001 atctctttca ggcgaccttt ataagctacg cgaccttcga taccttccgg caccagtttg
    30061 tcggcagcgt tatcgctctg gaaataacgg tcagaggaac ctttggacat cgcgcccagg
    30121 gaacccatac cacggtaaga tttgtaagaa cggccctggt agagttcgat ttcacccgga
    30181 gattcttcag tacccgccag catggaaccc accattaccg cgcttgcgcc agcggcgata
    30241 gctttggcga tgtcgccgga gaagcgaata ccaccatctg cgataaccgg aataccggtg
    30301 ccttccaggg cttcaactgc gtcagcaacg gcagtgatct gcggaacacc tacgccagta
    30361 acgatacgag tcgtacagat agaaccaggg ccgataccga ctttaaccgc gctgcaacca
    30421 gcttctgcca aagcgcgtgc acctgcagct gttgccacgt tgccgccgat gatttgcaga
    30481 tccggatatt tagcacgggt ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg
    30541 gaggagtcga tcagcagaac gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg
    30601 ttacccgcac ctgcgccaac tgcagcacca acacgcagac ggccttgctc gtctttacag
    30661 gcgttcggtt tacgttccgc tttctggaag tctttcacgg tgatcatgcc gatcaggtgg
    30721 aattcgtcat caaccaccag cgctttttca acgcgttttt cgtgcatttt tgccagcacc
    30781 acttcacggg cttcaccttc acgcacggtg accagacgct ctttcggcgt catgtaaacg
    30841 ctaaccggct ggttcaggtc ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc
    30901 agttcgtttt cttcggtaac gaccggatag cccgcaaaac cgttacgctc agtcagttct
    30961 ttcacttcgc gcagcgtcgt ggttggcaga acagtctgcg gatcagtcac cacaccagat
    31021 tcgtgttttt tcacacggcg aacttcttct gcctggcgtt caatggacat gtttttgtgg
    31081 ataaagccga taccgccttc ctgagccaga gcaatagcca ggcgcgcttc cgttacggta
    31141 tccattgctg cggaaagcat agggatattc agacgaatag ttttcgtcag ctgggtgctg
    31201 aggtcagcag tattcggcag aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac
    31261 gtcagagctt ctttagcgat acgtagcatg ggcaatatct cgaccagagt ggttaataaa
    31321 tattgccgcg gcattataca gagcgtaacc gattgcatct accccttttt gcaaaaaatg
    31381 cttgctatcc ccgaagggcg ggttactatc gactgaataa cctgctgatt tagaatttga
    31441 tctcgctcac atgttacctt ctcaatcccc tgcaattttt accgttagtc gcctgaatca
    31501 aacggttcgt ctgctgcttg agcatgagat gggacaggtt tggatcagcg gcgaaatctc
    31561 taatttcaca caaccggctt ccggtcactg gtactttaca ctcaaagacg acaccgccca
    31621 ggtacgctgc gcgatgttcc gtaacagcaa ccgccgggtg accttccgcc cacagcatgg
    31681 gcaacaagtt ttagttcgcg ccaatattac gctctacgag ccgcgcggcg actaccagat
    31741 aatcgttgag agtatgcagc cggccggtga agggctgctg caacagaagt acgaacagct
    31801 caaagcgaag ttgcaggctg aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc
    31861 ccctgcgcat tgcgttggtg tgatcacctc aaaaaccggt gctgcgctac atgatatttt
    31921 gcatgtgtta aaacgtcgcg atccttctct accggtgatc atctacccca ccgccgttca
    31981 gggcgatgac gcaccggggc aaattgttcg cgccattgaa ctggcgaatc agcgcaatga
    32041 gtgctacgtg ttgatcgttg ggcgcggcgg cggttcgctg gaagatttat ggagttttaa
    32101 cgacgaacgc gtagcgcggg cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg
    32161 gcatgagacg gatgtgacca ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc
    32221 tgccgccgct gaagtagtga gccgtaatca gcaagagtta ctgcgccagg tgcaatcggc
    32281 ccgtcaacgg ctggagatgg cgatggatta ttatctcgcc aaccgtacgc gtcgctttac
    32341 gcagattcat catcgcttgc agcagcagca tccgcagctc cggctggtac gccagcaaac
    32401 catgcttgaa cgcctgcaaa aacggatgag ctttgcgctg gaaaatcagc ttaagcgtgc
    32461 cgggcaacag cagcagcgat taacacagcg gctgaatcag caaaatccac agccgaagat
    32521 tcatcgcgcg caaacgcgca ttcagcaact ggaatatcgt ttagcagaaa tcctgcgcgc
    32581 acagcttagc gccacgcgtg aacgtttcgg taatgcagta acgcacctcg aagccgtaag
    32641 cccactgtca acgctggcgc gtggatacag cgttactact gctactgacg gcaatgtact
    32701 gaaaaaagtg aagcaagtta aagcgggtga aatgctaacc acacgtctgg aagacggctg
    32761 gatagaaagt gaagtaaaaa acatccagcc ggtaaaaaaa tcgcgtaaaa aggttcatta
    32821 agccagcacg aattcaacgc gttttttcga tatcaagcca tgcccgtgct gacagaagta
    32881 atctactgca ccgcaggctt ttaacacctg caacggttga tggcagtccg ggcaaagagc
    32941 tttcatttca ataatttccc cgcaggatgg gcaacgcgca tggccgttat cctgatcaag
    33001 cacatgctga cattgtggac aatgcagttc catatgaatt cctccggtag ccatccctct
    33061 acattcatag agggaatggc agataaaata cttacggata attatttatt tttcttgatg
    33121 tgcttcatca gacgcttacg tttacgcatc tgggttggcg tcagggtgtt acgcttgttc
    33181 gcatacgggt tttccccttc tttgaactga atacgaatcg gcgatcccat tacgtccagc
    33241 gatttgcgga agtagttcat caagtagcgc ttgtaggaat caggcaggtc tttcacctga
    33301 ttaccgtgaa tcaccacaat cggcgggtta taaccaccag cgtgggcata tttcagcttc
    33361 acacgacgac cgcgtaccag cggcggttgg tgatcttcaa cagccatcgt catgatgcgc
    33421 gtcagcatag aggtccccac acgacgggtg gagctgtcat acgcttcacg tactgattca
    33481 aacaagttac caacaccact gccgtgcaag gcagagataa agtgcacacg agcaaaatcg
    33541 ataaagccca gacggaagtc cagcgtttct ttcacctgct ctttcacttc ctggctcagg
    33601 ccatcccact tattcaccac aatgacaagt gagcgcccac tattgagaat aaagcccagc
    33661 agcgagagat cctgatcgga aataccttcg cgcgcatcaa tcactaacat caccacgttg
    33721 gcgtcttcaa tggcctgcaa cgttttgatt acggagaatt tctctacagc atcggtgatt
    33781 ttgccgcgtt tacgtacgcc agcggtgtca atgagcacat actcacgtcc atcgcgttcc
    33841 attgggatgt agatgctgtc acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct
    33901 tcaccaagaa tacggttagt gagtgtagac ttacctacgt tcggacgacc cacaatcgcc
    33961 agtttgatcg gcagactttg cgggtcgaag tcgtcttcct cttcttcttc gccgttctct
    34021 tccgcttcaa attgcgccca gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga
    34081 tcttccatcc acggcagcag cacatgctcc agcagactta atacgccacg accgtgagac
    34141 gcggcgatcg ggtagatttc acctaaacca agcgagtaga aatcaaccac tgcctgatcg
    34201 ggatccagac cgtcagtttt gtttgccacc aggaaggtcg gtttttcacg ggagcgcaga
    34261 tgtttggcaa tcgcttcatc tgccggcatc aggcccgcgc gcgcatccac cataaacagt
    34321 acgacgtccg cttcttcaat cgccagcagc gactgttccg ccatgcgggt ttctacaccg
    34381 tcttctgtgc catcaatccc gccggtatca atacagataa actcacggcc ttcaatttcc
    34441 gcacgaccgt acttacggtc acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg
    34501 gtgcgagtta gacggttaaa taacgtggat tttcctacgt tagggcgccc gacaagcgcg
    34561 accacaggta ccatgtttaa agcctcattt ttataaatca tcagacaacg cacgctatat
    34621 tcgcgtcgtt gttaaaaaca ggaaaacggc ccctgtccag gagccgtttt caaagtgaac
    34681 gacagagacg attaacgtgt aatagagtac acggttccgt cttttgcctg gatcagcagt
    34741 ttgccgtcag cggcaaccgg ttcagtctgg aaaccggaac tatcaacttt ttgctgggca
    34801 acgaaacgac catcttcgac gttaatccag tgcaaataac cttcactgtc accgaccacc
    34861 aggttgccat tatacagcac cggagaagtc agcaggcgat gcagcagatc gctttgtgtc
    34921 cacagcgtaa cgccgccatc aatggtcaac gccatcaccc ggtcattttg atcgaccaga
    34981 tagatgcgat tgccatcgac gatgaaatca ttcaccgaac ccagttcgcg tttccacata
    35041 atctgaccac tgcgcagatc aagcgccgtc aggttaccat tataggccag agcgaaaaca
    35101 acgccgttaa caacgacggg agtcgtgtca acatcgctca gacggtcaat ttcggtagaa
    35161 ccggtcgcct gggaaatacg ctgctgccaa atcatctggc cctgttccat cagcactgcg
    35221 ctgacgcgac cattatcgcc acccacgacg gccgcaccaa aagccgttgc cggcgcagac
    35281 tcgccacgca aagagagcga aggcatatcg aggttaactg tccatttgac agcgccgtca
    35341 gcttcgttca gcgcttgtaa ctgaccgtta ctggtgtgga ttaacaccag accgtcgctg
    35401 accaccgggc gcgaaagtgc ttcacccgcg actttagttt gccatgccac agtaccatcg
    35461 ctggtattca gcgcgtaaac ctgcgccttt tcgctgccaa tgtagacatg cccaccagac
    35521 acggtcacac cgccagaaag taatgcaggc tctttagaga accagccatc tttctcggcc
    35581 aggctgacag accagatttc tttgccatca tccgcattca gcgcttttac caaaccagcg
    35641 cggtccgctg catagacaac gttgtccgcc agtgccggat gaagattgga atagaagttg
    35701 ccaatgccgc taccaacgga agtgctccac gccgtggtcg gcgtaaactg gttttcaacg
    35761 gttggcaatg gggacatctt taccacatct tcttcgctgt taaacagcga acagccgctt
    35821 aaaagggtaa cggaaagcag tcctggcagc agtaatttac gcaattgcat cgggtccctc
    35881 tcagatggac aaattattaa ttttcatctg catcatttcg ctcagtgccg gagtaacatc
    35941 gcttttcacg cctgcttccc atgcactacg cgcaccttgc ttatcacctt tgctcagcaa
    36001 tgcttcacca cgcaggtcgg caacaatggc agcccaccct tcacctttga tggtatcaag
    36061 ggttttcagc gcggcatcag cctgcttgag ctgtacctga acgcgagcaa gacgcagatt
    36121 tatcacggct ttgagatttt catcgctcgt gtctgccagc ccctgttgta actgggcggc
    36181 agctttctct agttcatttt tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc
    36241 accataagta tttttatttt cagcagcaaa tttttccgcc gccgggatgc tatccggttt
    36301 gccttcgctc actgcggtaa ccgcattttg ataggcaaga gaagcggagc gtgcagaatc
    36361 aacctgatgg ctgttccagt agcgccagcc aatcagtgcg ccaacgccca aaatcacccc
    36421 aacagccagt gctttgccat tttcagcaaa aaagcgttta accgcttcta cctggtcgtt
    36481 ttcgttctcg taaatttcca cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa
    36541 tgcgcggcta cgctatcctg cgcaactgcc gtttgctcac cagagcgcaa atccttcact
    36601 actgctgtgc cgttagccac ttcagactca cccagcacca cagcaacgcg ggcaccccat
    36661 ttatcagcac gggcaaactg tttcttaaag ttgccgccgc cgtggttggt catcaatttc
    36721 acgcccggta attcatcacg cagacgctca gctaatgcca tagccgcaga ttgtgtatca
    36781 gcacctgaag ccaccaggta tatatcgaca acaggatcgg ctttaaattc cggattaacg
    36841 gcctgtacta acaatacaag acgttcgagg cccatcgcaa aaccgacagc cggtgttgca
    36901 cgaccgccca gttgttccac aagaccgtca taacgaccgc ctgcacacac ggtgccctgg
    36961 gagccgagac tgttagtcac ccactcgaaa acggtacggt tatagtaatc cagaccacgc
    37021 accagacgct ggtttacggt gtaagcgatc cccgcgctct caagcagttt gcacagaccg
    37081 gcaaaatgct cacgagattc ctcgtccaga taatcaccta atgccggagc gtcgttgaga
    37141 agcgcctgca cttccggatt ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg
    37201 cgtttgcagt cttcgtccag cttatcttta tactgctcaa ggaatgccac cagcgcatcg
    37261 cggtaattgg cgcgtgcttc cagcgaaccg atagagttca gctcaagagt tacgtgctcg
    37321 gaaataccca gcgcacgcca ccagcgggcg gtgagcataa tcagttcagc gtcgatatcc
    37381 ggaccttgca gaccgaaaac ttcgcagccc aactgatgga actgacgata acgccctttc
    37441 tgcggacgct cgtgacggaa catcggcccg atataccaca gacgctgttc ctgattgtac
    37501 agaagaccat gctcgatgcc ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc
    37561 aggctgtcgc cattgcgatc ctcaaaggtg tacatctctt tttcaaccac gtcggtgact
    37621 tcaccaatcg cacgtttgaa tagcggggtc tgctctacaa tcggcaagcg gatttcactg
    37681 taaccgtagc tgccgagcac gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt
    37741 tcgccaggca ggtaatcgtt catgccgcga atggcttgaa tgttttttgc cacgtttatt
    37801 ctctttctga atataaaaat gaaccctcaa cgcttccctc aatgtttggg gagccatgcg
    37861 ggttcaatca tacacgggaa gcgaggcgct tcccatcacg ttattatttt tcaacctgct
    37921 gaacgtcaat tcgacgcgct tcatccagct gactggcttt cgcacgaatg cgtgcttcca
    37981 gctggtcgat catatcgttg ttgtccagac ggtctttgcg cacgccatct tcatagaggc
    38041 cgcttttctt gttgccgccg gtgacgccga gtgtagaaac cagcgcctca cctgggccat
    38101 tcaccacgca gccgataatc gaaacgtcca tcggagtgat gatatcttcc aggcgttgct
    38161 ccagcgcgtt aaccgtaccg ataacatcaa attcctgacg cgaacaggtc gggcaggcga
    38221 tgaagttgat cccgcgcgaa cggatacgca gcgatttcaa aatatcgaaa ccgactttga
    38281 tctcttcgac cggatcggcc gccagtgata cgcgcagcgt gtcgccgatg ccttcagaca
    38341 gcagcagacc taaaccaatg gcggatttta ctgccccgct gcgtgcacca ccggcttcgg
    38401 tgatccccag atgcagcggc tgatcgatct gttttgccag caaacgataa gactcaacag
    38461 cgaggaagac gtcagacgct ttcacgctga ctttgaactg atcgaagttc aggcgatcga
    38521 gatgatcaac atggcgcatg gcggattcca gcaacgcctg cggcgtcggt tcgccatact
    38581 tttcttgcag atctttttcc agcgatccgg cgttaacgcc aatacggatc ggaatgtttt
    38641 tatcgcgcgc acagtcaacc accatgcgaa tacgctcttc attaccgata ttgccagggt
    38701 taatacgcag acaatcgacg ccgtattccg ctactttcag tgcaatgcga tagtcgaagt
    38761 ggatgtcagc caccagcggc acgttaacct gctgtttgat gagtttgaac gcttctgccg
    38821 cgtccatcgt cggtacggag acacggacga tatcagcgcc aacgcgttcc agcgccttga
    38881 tttgattgac cgttgcttcg acgtctgtcg tacgcgtatt ggtcatggac tgtacggcga
    38941 tgggagcacc atcgccaatc ggcacattcc caacgtaaat acgtgttgat tttctacgtt
    39001 gaattggagc ctggttatgc atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg
    39061 tgattgttcg gcattgaggg tcagacgcgc aacctggtta gttctgataa aacgactcag
    39121 atcgacaggt ttcccttgat actggatctg tactgcggct ggcgcaccaa ttttcagttt
    39181 gtatggtgcc tggccggtta agttcaaatt accgtcttta cgctgcatac cgctaaacaa
    39241 ttttttaccg gtagcatcag taacctccag ccagcaatcg gcagtaaagt tcatcaccag
    39301 cgcattcgga tcagccgccg gcgtagtcac gcctgcctga tcggttggca acggcgcagc
    39361 gccatctggc gttgttgttg ccgttggtgc cggggtcgcg gcggtatcaa catttgcctg
    39421 cgaaggcgaa acaaccgcat tctgttgcgg atcaacagct ggtgctggcg cagttacggc
    39481 aggtgtttgc gtgttggttg cggtagtatc cacagacgct ggcggcgtgg acgttgtagc
    39541 cgggtctgta gttgtcgacg tatttaacgg aacactctgc ccctgctcgc tattactgct
    39601 cagttccgcc gaagattgat cggccatagt ggtgatctct tcctgctgag ctttgtggtc
    39661 ttgccaccac caggcaccgc tcaggccgat aaccacaaac aacaccagcc aggtgaaggt
    39721 catcagccag ccgtcacgtt ttttgcggcg tttaccgagg gaaaaactct gcatcggcgc
    39781 aactttcgca gcccgtagtg gagcctgctt ttccagccct ggcagcagtt cttcttctgg
    39841 aatatgtacc agacgcgcat aagagcggat atatccgcgc aggaatgttg aagcaagatc
    39901 ggcgggtgcc ttatcttctt caatgtcgcg taccgtggaa accttcaggc aaagtcgctc
    39961 ggcaacggcc tgctgactaa gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt
    40021 agtaagtgct tcattttggt cgtgcgtggc ttcagtattc attcgctgca ggtacgttta
    40081 aatgagaatt gagatgccgg tgaaccatca tgctcaccca caccgcgaaa catccgttaa
    40141 gttaaccgtt atcatacagt ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac
    40201 taaggctaac ttactgttgc atcgttacat actgccttaa agtcagcaaa aacgcaccgt
    40261 taatattgac cagacaattg caacttaata cttcattcat aaaagattac gccacggtac
    40321 ataaagtaac cgtggcgtaa tggctatcag accgctttaa tgtcgatggc ttcaccctgc
    40381 atccgtttac gcagggtacg tttcgtacgg tcgataacat cgcccgccaa ctgaccacag
    40441 gcagcatcga tatcatcacc acgggtttta cgcacaatag tggtgaaacc gtagctcatc
    40501 agcacttttg agaaacggtc gatacggctg ttcgagctgc gtccatacgg cgcgcccggg
    40561 aacgggttcc acgggatcag gttgatctta cacggcgtat ctttcagcag ttccgccagt
    40621 tggtgcgcgt gttcagtgcc gtcgttaacg tggtcaagca tcacatactc aatagtgaca
    40681 cggccctgat tggcgttgga tttctccaga taacggcgca ccgcagcaag gaacgtttcg
    40741 atattgtact ttttgttgat cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc
    40801 agggaaattg ccagtgcaac gtcgatcata tcgcccagtt tatccagcgc cggaactaca
    40861 ccggaagtag aaagcgtgac gcgacgttta gacaggccaa aaccgaaatc atcaagcatg
    40921 atttccatcg ccggaacgac gttgttcagg ttgagcagcg gctcgcccat gcccatcatc
    40981 actacgttag tgattgggcg ctgaccggtg acttttgctg cgccgacgat tttcgccgca
    41041 cgccacacct ggccaataat ttccgatacc cgcaggttgc ggttaaagcc ctgctgggcg
    41101 gtggaacaga atttacactc cagcgcacac cccacctgcg aagagacgca gagcgtggca
    41161 cggtcgtctt ccgggatata caccgtttcg acgcgctgat cgcctacggc gatcgcccat
    41221 ttaatggtgc cgtcggatga acgctgttct tcaaccactt ccggtgcgcg gatttccgcc
    41281 acctctttca gtttgccgcg caacactttg ttgatgtcgg tcatctcatc aaagttgtcg
    41341 cagcaatagt gatacatcca cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa
    41401 tctttaaaaa actcccgcat ctgctgacgg ttgagatcca gcaggttgat ttttccatct
    41461 ttcgtggtga cgttttcagg tgtgactaat tgttcagaca tatgctattc cggcctcgtt
    41521 attacacgtt atggcccctg gagggttgaa aaaagaaacg ccccggtgag cggctgctcg
    41581 tccgggggcg ctgcattgta caaattctgg cgcacggatg ccacgtttgc acgcggcatt
    41641 tacgaaatta ttaacgggtg cgcgggcaca cttcgccttc gccaaagaaa taagcgattt
    41701 cgcgagcggc agattcgacg gaatcagaac cgtgggtacc gttttcggtc aggctgtcag
    41761 cgtaatcagc gcgcagagta ccagccagtg cgtttgccgg attggtcgcg cccagcagat
    41821 cgcggtgacg ctgaacggcg ttttcacctt ccagcacgga aaccacgatc gggccagagg
    41881 tcatgaattc aaccagacca tcaaagaacg gttttccatc gtgttcagca taaaagccac
    41941 gtgcctgttc aacggtcagg tgcagcattt tggtgccgac aattttgaac cctgcagctt
    42001 caaagcgcgc aaagatatta ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg
    42061 aaaaagtacg ttcaatagcc atttttacct ctgtaaattg ttctgttgtt gtctgtacca
    42121 gcgtacgaga tggcgcggat tataatgagc aacagggccg ttgactattg atgaaggtaa
    42181 cattttttta aaataaaatg agttttagca acaatcagga gcaaaacaga ctattgcatg
    42241 acaaatttca ctgtcgcgat ttgccccaca tcatccatca ccagcaattg ataatcacct
    42301 ttatccgtca aatgcagggt cacgttgcgc ccgcgttcag ttaacggttc gccattcaga
    42361 aaccaccagc gttcacctgc ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg
    42421 cccggtaaac gtttaataat cgcgccatcg cgaacgcctg tcagctgcag agggagcttg
    42481 gcatcgtgac cgtatggcgg gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt
    42541 tctgatgcgg gcagccaggg ttccagcggt agcggccaga catttatcat ttcttgtcgc
    42601 gcttgcgggc aatcagcggc aacacgtttg ccattttcat ccagccagat ggggaaacga
    42661 atgccattaa tgccttcctg ctccggcagt aatagagttg gcggctgact cccgtccagc
    42721 agccaggttg ccaggcggcg gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc
    42781 ggccagcaga taacgccacg agtgactgag ttcgggcgcg ggtcttccgg cagattcgca
    42841 ctgcgcgaca gtaagatatt attgacctga ttcaacaatg gtacggcact ggcaaagcca
    42901 aactgaccaa caacgggcgt gccgtccggt ctgccagtcc agatcccaat gacatagcga
    42961 gcgttaaccc caatcgccca ggcgtcacga tagccatagc tggtgcccgt tttccatgcc
    43021 agtggggcga cgcgcggcaa ggcactatcc ggcaagggtt tcgcttcatc agccataatc
    43081 cggcgaatga tccacgccgc ccccgacgac attaaaggcc gttcaagcag cggatcgtca
    43141 ggctgtaagc gcaatttgcc tgccttgccg tggcgagcaa acgcggtata cgctgccgcc
    43201 atatcttcca gttttgcacc agcgccgccg agaatgagtg aaagattcgg cgcagcaccg
    43261 ttgggcaaat ataacggcaa tccaacattg cgtaactttg ccgcaaaccg tttcggtcca
    43321 taggcttcca gcacctgcac agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg
    43381 ctgatcgggc catgaaaacc gctatcaaag ttacctggtc gataatcacc ggtgcgccgg
    43441 gggacgtctt gcagcagtga tgccgggtgg atcaagcctt catccagcgc cagaccataa
    43501 acaaacggtt tgagcaccga tcccggcgat cggatcgcat tgaccatatc aacatgacca
    43561 aagcgtgaat catcgttgag atcaaccgat cccacccagc cgcgaacacg catatcggta
    43621 tgatcaacca cgatcatcgc cagtgagctg cgcggtggca accgcccttt ccagttttgc
    43681 gccagttctt ccagacgtcg ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt
    43741 ttgcttttac cgagcatcat gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt
    43801 gccagccaga tgggttcttc ccttgactct tttacctgct cacgggacca cacaccttgc
    43861 acggccatcc gttcgagcac tttattacgc gcggcttcgg cacgctccgg ccaacgatcc
    43921 gggcgaagac ggctgggcgc ttgcggcaaa accgccagca ttgccgcctc ggaatagctt
    43981 aaattcgcag gcgactttcc gagataagcc caacttgccg caccgatccc ctgcaacgta
    44041 ccgccaaacg gagcgcggtt aagatacaag gtcagaattt cacgcttaga cagatgccat
    44101 tccagttgca acgcgcgcca gagctggcga attttgccgc caaatgtttt ggggtgagga
    44161 tcaagcagac gagcaacctg catagtgagc gtgctgccac cggaaataac ccgtcccgaa
    44221 gtgagatctt gccatgctgc gcgcgccacc gagaatggat tcacccccgg atgcttccag
    44281 aaccagcgat cttcataatt gatcagcgct tcaaggtaac gtggagaaac atcttcgatt
    44341 gttaccggat aacgccagat gccgtcagca tcggcgaagc gccatagcgg cgtaccatcc
    44401 tgcgccacga ccactcgtgc gggattgact tcatgcagcg gtagaggcca gagtttatct
    44461 gctccccacg ctgcaagaaa aaggagaaat ggcgcagctg ccagcgttat ccagcagccg
    44521 cgtttggtta acaagcgagg catttacggt cggacaatca gcagatcttc agccgcgccg
    44581 gtcgcccgcc attggggaac atacattgat tccaccatcg gttgcggtac ctgatacgtt
    44641 cccggcgtca ccgcccgcgc cagatacacc agcgttaccg gttggtattc atcaacggca
    44701 accgccgcca caaagcgatc gtcacggaac tcaatgtgct taatgctcgc ctgctgcatc
    44761 tggttcagta agttttgcac ttcgccacca ctttgctcca ggctggcgct accgttcgcc
    44821 agattctgat tttccagttc cagacccgca ggcagcagat ccacgactaa cgcatccggc
    44881 acgctgttac tggcttttac ctgcaaccag accagcacca gatcgccgct acgtaacgag
    44941 tccagcgatt tgctcttacc atcagtacca agaatatgac gctcgatttg cagcacattg
    45001 ttcgccggta aaggtgcgga ttgcggataa ccgctggcat ccatacgcaa ccataacggc
    45061 tgatcgccac tgtttctcac ctgcaaggtg gcaagttgat cgctattcag attgctgttt
    45121 agcgttttct cgcctgtcag cggctcagct gagaaagagg tttgcgcctg ccatttaccc
    45181 ggtaaatcct gaatcgtacg ggcagccagg aacaacgcgt tactttcctg cgtcgatagc
    45241 cagcgttcac caaacgcctg ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt
    45301 agcagtttat tttcttccag caaggagagc attaacgcgt tgtcgcgcag tggactaccg
    45361 taatcaccca gccatatccg ctcatcacta tttctcggcg ttttcagcgc cagcacaatc
    45421 gcttcttcac cacgcgttgc atcacccatg gttttcagcg caacgccaag ttgcagcagc
    45481 ggtaaaccag aagcggcatc tgcacgatgc tcccagattt cacgcagcgc acccagcgga
    45541 gccttttgtt gacgggccaa caccagcgca gcgtaagact gtacggcgaa tttactggct
    45601 ttgagattat ccgcgtacgg gatcgacatc atgcccggat cttgtaaata gcgcagcaga
    45661 cgctcattac cccggttaat ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg
    45721 accaggaaat ccatcacgta agccgtcagc cagtactctt cgtcaccgtt tttatcccac
    45781 agcgcaaagc cgccgttatc acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc
    45841 gatgcacggc gtttctcatc actgtcgcct ttgatgccca acgcctgcag ttgggctgcg
    45901 ttggtataaa gtgacgggaa caggccgctg gcggtttgct caagacagcc gtacggatac
    45961 gcttttaact ctttgatata acgtgcaatg ttcagcggtg gtttgccgct caacaacaat
    46021 tgcccttcca gcgtaacagg cgagaagttt tgcaatccat ccgccggaat cgcccatgtc
    46081 tcaccaggct gtaacgccgt accgtaatta accgtttgtg ccgggaacgc cggacggacg
    46141 ccgattttcc actgcttctg ctgatcggca acggtttcat ccggtaacgc taacccgcta
    46201 atggtggcct gaatttctcc atcgccataa cccggcaatg ctcgcaccgg gataaacagc
    46261 gtagtacgca cacctggcgc taattcaacg gctgcgggtg aatcgctgac cagttcaagc
    46321 aaaccactgg cggtcagggc aacgttcagt ttttgcggtt tatcggtaag attagtgata
    46381 tccagcgtca gacgcgaggt atcgccactc gccataaagc gcggcatgtt cagttcagca
    46441 atcaccggtg cggcaactat cactttgctt tcgttgctac cgaaatcatc tgccgtccag
    46501 gcttgcgcca tgacgcgcaa ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct
    46561 tcgccctgtt cgttgagcgt taccggcagc gcctgctgcg cgacaatatt gacgtgattg
    46621 accggcggtt taccaccacg tttcagctca tcaccatcgc caccgaaacg cagagctgcc
    46681 agacgcccct gaccttcaat aacctgaccg taaatatcgt aaatgtctgc gccatagcgt
    46741 ttctgaccaa agaacgcctg ccacggatct ggcgtgacgt agtcagtaat attcagcaca
    46801 ccactatcaa cggcagacac cagcacattc acctgtttag gcttctcgcc atttttagtg
    46861 ctggctttaa ttttcacggt taatggttga ttgggacgca tttttgctgg tgtttccagc
    46921 gccagatcga ggcgacggtt ttcatcgcca agcggcagat gcaacacacc aaccgcgcgt
    46981 tttggcgtcg cggagcgaga tttatcgcca ggacgtacca ccagcgtact taaatacaga
    47041 tcatgacgat tccaggtttt atcgaccgga atcgtcagat ccagcccttg agcccgaaca
    47101 tcaatctctt gccaccacag cggcccttca ctggactcga ccatcgcata acctttaccc
    47161 gccgttggcg cggcgatatg caacttaatg gtgtcgccag ggcgataact ggctttatcc
    47221 agtttcagcg tgacacggtc gggtcgcact gcgccgctac cgtcgctgtt gtcctgccag
    47281 ctatagccag cccagaaacg aacactactg accgcttcat tcggcgcttt gacttccaga
    47341 cgataagcgc cccactctac cggaaaactg accttgccgg tttcgtccgc tttcagatcc
    47401 agagtttgtt cattttcgat cagatctttt tgatcaaact gtgactgcca gccttcatct
    47461 tctgaccagt tccagtagta atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac
    47521 acggcttttt tcacgccttg cgcatcgcta taaacgatgt caaaagcggc gttactgcct
    47581 tcatcaacaa tgggctgttt caccgtgctg tcagtacgat aatcgtaaac cgatttcgag
    47641 gcgaactgcg gacggatccc cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg
    47701 cgcgtcaccg ggcgaccgcc cgattccagc aaactaccct ggaaaataac ctgtaatggg
    47761 gaatgcgttt ccttccactg gctttctgta gaaacttcgc cgcgcccttt atcatccagc
    47821 gtcaactgaa cttcatccag cgtgcgggaa agattttcgg cagctatatc gccgaattca
    47881 aaaccaggta aggctgacac agcttcacgc agtgggcgca ggaaaagttg cccttgcaaa
    47941 gtattaccat tagcaggtgc accatacagg tagtacccca ccacggagaa tttcacttca
    48001 tctttcggcg ttagcggggt tttctcaccg gtcagattca gcgccatgcg ctctggcata
    48061 aaatcttcga cgtggaaatc ccacatccga tactggttat cgcccgtgtt agcgcgaata
    48121 tgccacatac cggttgccgc attgctatcg agtggccagg taaagtggta gaggccattt
    48181 tccggctgac taacgacgct cctgagtacc tgcccatcgg gtttaatcac gtctaacttg
    48241 atgggttgat tgggcaacgc tttaccgtct gcatcacgca gcaaaccatt gaggattacc
    48301 gtttcacccg ggcgataaag atcgcgtggg ccaaacatga aaaactgttt gctatagcct
    48361 ggcgcgccag caatgttaaa ttctgctaag tccagcgccg gaagttttaa atcgagtagc
    48421 gttgtctgac cgtctttacg cgccaacagt aatgccgcgt ttttatcatt ttccagctgc
    48481 acatgcccct gagcgtcact ggttgcctga gtcagagtct gccctttctc atttaataaa
    48541 gagacttcaa ttccttgctg ggccgcgccg ttttccagac tttgggtaaa gatatccaga
    48601 cgattgtgat aacggtgagc tgaaacgccg atatcactta acgtaaacag cgtcgcggga
    48661 ttactgtaat cgtaacgtcc agcctgattc atcacagcca gatacacgcc cgcctgctga
    48721 agcggtttga tatcgcccag cggcagcaat aatttttcac gggtgttacg cgcaggattg
    48781 agatcaaacc gtccggtgta gaccagatcc gccatctgca gcagtttgtc tgactgccag
    48841 ttcgccagcg aattgcggta ttcccattgg ctaatgaatg ctggcagaga ttctggctta
    48901 acgcggaaga agttaacatc gacattatta acgttgagcg ccattaccgg cagcccttca
    48961 acgactttac caggcagcag cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg
    49021 cgggtagtta tagttttttc gtaatcttta ctgaaggttg cgttgttgag tgctttgacc
    49081 tccttgccaa tagtaacgat caaatcacgt ttcggttcga ggtggcgtaa acgtaactct
    49141 ttaagattat ctgacagctc ccaggcacca tccactttgc cgcttttttt atcgacgaca
    49201 tgaataacgc gtgagaaatc ctgatccggg tcgagaggga tggagaacgt cagcaccagc
    49261 gtggctgcac catcaagttg gacttcagag agatccagca atgtcagcac cttaccggca
    49321 ctctgttggg ccagtttttg tctctccggc acggagagtt ttgctgaact cgcgttttct
    49381 gaagaggccg ctttagtaac ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta
    49441 tcgttgttgt cgcaccctgc cagcgccagc attagcatgc aggcggctac gcgtaacttt
    49501 ttcatttttc atccctgcca caatggcccg ttagcaacgt cgaataatta ttatgcgtga
    49561 gaatttacgt tatgtaattc agtatcacgc ccatacaatc cgaattttag tgagaattgt
    49621 ggctcagaca gccaaatcag cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt
    49681 tagcctaacc ccggcaaaaa tggagatgcc tatgtccacg acatggtttg taggagccga
    49741 ctggctcgcc gaacatattg atgacccgga aattcagatt atcgatgccc gcatggcgtc
    49801 gcctggacag gaagatcgta acgttgctca ggagtatctg aatggacata ttcccggcgc
    49861 agtgtttttt gatatcgaag cgctttctga tcacacttcc ccgcttccgc acatgctgcc
    49921 gcgcccggaa acgttcgccg tggcgatgcg tgaattaggc gttaaccagg ataagcacct
    49981 gattgtctat gacgaaggta atcttttctc agccccacga gcatggtgga tgctgcgcac
    50041 ctttggtgta gagaaagtgt cgattctggg gggtggactt gcaggctggc agcgcgatga
    50101 tctgctgtta gaagaaggtg cagtagagct gccggaagga gagtttaacg ccgcgtttaa
    50161 tcctgaagcc gtggtgaaag taaccgatgt attattggca agccatgaaa atacggcgca
    50221 aattattgat gcccgcccgg ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg
    50281 tttacgtcgc ggacatattc ccggagcact gaatgttccg tggacggaac tggtgcgcga
    50341 aggcgaacta aaaacgaccg atgaactgga tgcgatattt tttggtcgcg gcgtcagcta
    50401 cgacaaacca attatcgtca gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc
    50461 actcgcgacg ctggatgtgc caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg
    50521 cgcgcgggca gatttaccgg ttgagccagt gaaataagta ttttacaggc aataaaaaac
    50581 cgccgaattt ggcggttttt tattgctggt ctggttcgca gcctttccag caggttgtat
    50641 taccgtagta atgcaagcgc gtctcagcgg agacaatact cgccagtaac tctctttttg
    50701 tcaagcaaaa gagagttatt attgttctgt tagtgtatta tccactgcgg ccctttccgc
    50761 cgtctcgcaa gcgggcgctg gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc
    50821 gtctcagcgc ttgctatcgc ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc
    50881 atggaccgca caatgaagat acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg
    50941 ttttcccaag taaataataa ccggagtctc tccggcgaca atttactggt ggttaacaac
    51001 cttcagagca gcaagtaagc ccgaatgccg ccctttgggg cggcatattt tagattatcc
    51061 gattctgttt aaagtcacgc aaaaaaccac cccagcgacg ttcatagaat ggcacaatat
    51121 gttcggtaat aaagtggcta attccttttt cccctttttt cacctgacaa atatcgattg
    51181 gttcatcgcc aggtaatgta tcggtcgcta cacttcccgt cgcctgaata atttcttcga
    51241 tatcaccatc ggcttcaatg ccaataagta aattaggctg tgcctcttcg ttctctttaa
    51301 ttgaacaaat aaaagcacgc ttcaccggct taatggtttt aaataaggtg gtgagtgaat
    51361 caatcatttg tgctggcggc tctgcgactt ccgataatat cagcgattca ccgccttcca
    51421 ggatttcctg gctgctcagc ggatttccct cttcaccaat caacaaactg atttcacgcg
    51481 gcataaattc tttaccggtc ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca
    51541 tctcaaacaa tgtgcgaacg ggcattacga caaatgcctg ttcgtcttca accgcctgtt
    51601 gaagcgcttc taacgaggtg aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt
    51661 gctgtaaatc aagcgcacta tcttcaacca cagcctcgcc ctgcgccgcc gtacccggca
    51721 cccagacggt ggatcccagt agagtacgga aaaaggccgg gcgatgcgcc ggttcggttg
    51781 ctgctttttc cagcaggtct tcaagttcgt tttttgtttc ggacataaga accacaattc
    51841 attcaacgtt cggggcacaa
//
LOCUS       DAEVZA010000018        51504 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEVZA010000018 DAEVZA010000000
VERSION     DAEVZA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.018"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..322)
                     /locus_tag="LAF92_001100"
     CDS             complement(<1..322)
                     /locus_tag="LAF92_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBI9790146.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMM"
     gene            complement(322..936)
                     /locus_tag="LAF92_001101"
     CDS             complement(322..936)
                     /locus_tag="LAF92_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBI9790147.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(926..2917)
                     /gene="cyoB"
                     /locus_tag="LAF92_001102"
     CDS             complement(926..2917)
                     /gene="cyoB"
                     /locus_tag="LAF92_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HBI9790148.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(2939..3886)
                     /gene="cyoA"
                     /locus_tag="LAF92_001103"
     CDS             complement(2939..3886)
                     /gene="cyoA"
                     /locus_tag="LAF92_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBI9790149.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(4346..5821)
                     /gene="ampG"
                     /locus_tag="LAF92_001104"
     CDS             complement(4346..5821)
                     /gene="ampG"
                     /locus_tag="LAF92_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBI9790150.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(5865..6443)
                     /locus_tag="LAF92_001105"
     CDS             complement(5865..6443)
                     /locus_tag="LAF92_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI9790151.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            6748..7065
                     /gene="bolA"
                     /locus_tag="LAF92_001106"
     CDS             6748..7065
                     /gene="bolA"
                     /locus_tag="LAF92_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBI9790152.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            7409..8707
                     /gene="tig"
                     /locus_tag="LAF92_001107"
     CDS             7409..8707
                     /gene="tig"
                     /locus_tag="LAF92_001107"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBI9790153.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            8953..9576
                     /gene="clpP"
                     /locus_tag="LAF92_001108"
     CDS             8953..9576
                     /gene="clpP"
                     /locus_tag="LAF92_001108"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBI9790154.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            9702..10976
                     /gene="clpX"
                     /locus_tag="LAF92_001109"
     CDS             9702..10976
                     /gene="clpX"
                     /locus_tag="LAF92_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBI9790155.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            11164..13518
                     /gene="lon"
                     /locus_tag="LAF92_001110"
     CDS             11164..13518
                     /gene="lon"
                     /locus_tag="LAF92_001110"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBI9790156.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            13727..13999
                     /gene="hupB"
                     /locus_tag="LAF92_001111"
     CDS             13727..13999
                     /gene="hupB"
                     /locus_tag="LAF92_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBI9790157.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            14191..16062
                     /gene="ppiD"
                     /locus_tag="LAF92_001112"
     CDS             14191..16062
                     /gene="ppiD"
                     /locus_tag="LAF92_001112"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI9790158.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            16213..16584
                     /locus_tag="LAF92_001113"
     CDS             16213..16584
                     /locus_tag="LAF92_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBI9790159.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            16678..17076
                     /gene="fadM"
                     /locus_tag="LAF92_001114"
     CDS             16678..17076
                     /gene="fadM"
                     /locus_tag="LAF92_001114"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBI9790160.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(17128..17823)
                     /gene="queC"
                     /locus_tag="LAF92_001115"
     CDS             complement(17128..17823)
                     /gene="queC"
                     /locus_tag="LAF92_001115"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBI9790161.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(17888..19588)
                     /locus_tag="LAF92_001116"
     CDS             complement(17888..19588)
                     /locus_tag="LAF92_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBI9790162.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            19688..20506
                     /gene="cof"
                     /locus_tag="LAF92_001117"
     CDS             19688..20506
                     /gene="cof"
                     /locus_tag="LAF92_001117"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBI9790163.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            20659..21117
                     /gene="decR"
                     /locus_tag="LAF92_001118"
     CDS             20659..21117
                     /gene="decR"
                     /locus_tag="LAF92_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBI9790164.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            21147..22919
                     /locus_tag="LAF92_001119"
     CDS             21147..22919
                     /locus_tag="LAF92_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBI9790165.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESFTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            22912..24693
                     /locus_tag="LAF92_001120"
     CDS             22912..24693
                     /locus_tag="LAF92_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBI9790166.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            24874..25212
                     /gene="glnK"
                     /locus_tag="LAF92_001121"
     CDS             24874..25212
                     /gene="glnK"
                     /locus_tag="LAF92_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBI9790167.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            25242..26528
                     /gene="amtB"
                     /locus_tag="LAF92_001122"
     CDS             25242..26528
                     /gene="amtB"
                     /locus_tag="LAF92_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBI9790168.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(26576..27436)
                     /gene="tesB"
                     /locus_tag="LAF92_001123"
     CDS             complement(26576..27436)
                     /gene="tesB"
                     /locus_tag="LAF92_001123"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBI9790169.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            27654..28226
                     /locus_tag="LAF92_001124"
     CDS             27654..28226
                     /locus_tag="LAF92_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBI9790170.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(28257..28568)
                     /locus_tag="LAF92_001125"
     CDS             complement(28257..28568)
                     /locus_tag="LAF92_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBI9790171.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            28731..28827
                     /gene="ffs"
                     /locus_tag="LAF92_001126"
     ncRNA           28731..28827
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LAF92_001126"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            28947..29300
                     /locus_tag="LAF92_001127"
     CDS             28947..29300
                     /locus_tag="LAF92_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBI9790172.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(29342..30892)
                     /gene="pdeB"
                     /locus_tag="LAF92_001128"
     CDS             complement(29342..30892)
                     /gene="pdeB"
                     /locus_tag="LAF92_001128"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBI9790173.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSAFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(31056..31526)
                     /locus_tag="LAF92_001129"
     CDS             complement(31056..31526)
                     /locus_tag="LAF92_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBI9790174.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(31642..32193)
                     /gene="maa"
                     /locus_tag="LAF92_001130"
     CDS             complement(31642..32193)
                     /gene="maa"
                     /locus_tag="LAF92_001130"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBI9790175.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(32365..32583)
                     /gene="hha"
                     /locus_tag="LAF92_001131"
     CDS             complement(32365..32583)
                     /gene="hha"
                     /locus_tag="LAF92_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBI9790176.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(32609..32983)
                     /gene="tomB"
                     /locus_tag="LAF92_001132"
     CDS             complement(32609..32983)
                     /gene="tomB"
                     /locus_tag="LAF92_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBI9790177.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(33529..36678)
                     /locus_tag="LAF92_001133"
     CDS             complement(33529..36678)
                     /locus_tag="LAF92_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBI9790178.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(36701..37894)
                     /gene="acrA"
                     /locus_tag="LAF92_001134"
     CDS             complement(36701..37894)
                     /gene="acrA"
                     /locus_tag="LAF92_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBI9790179.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            38036..38683
                     /gene="acrR"
                     /locus_tag="LAF92_001135"
     CDS             38036..38683
                     /gene="acrR"
                     /locus_tag="LAF92_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBI9790180.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            38811..42173
                     /gene="mscK"
                     /locus_tag="LAF92_001136"
     CDS             38811..42173
                     /gene="mscK"
                     /locus_tag="LAF92_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBI9790181.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLAGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(42385..42546)
                     /gene="rsmS"
                     /locus_tag="LAF92_001137"
     CDS             complement(42385..42546)
                     /gene="rsmS"
                     /locus_tag="LAF92_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBI9790182.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(42560..43087)
                     /gene="priC"
                     /locus_tag="LAF92_001138"
     CDS             complement(42560..43087)
                     /gene="priC"
                     /locus_tag="LAF92_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBI9790183.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            43157..43534
                     /locus_tag="LAF92_001139"
     CDS             43157..43534
                     /locus_tag="LAF92_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBI9790184.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            43687..44238
                     /gene="apt"
                     /locus_tag="LAF92_001140"
     CDS             43687..44238
                     /gene="apt"
                     /locus_tag="LAF92_001140"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBI9790185.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            44357..46288
                     /gene="dnaX"
                     /locus_tag="LAF92_001141"
     CDS             44357..46288
                     /gene="dnaX"
                     /locus_tag="LAF92_001141"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBI9790186.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    45621..45685
                     /gene="dnaX"
                     /locus_tag="LAF92_001141"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            46341..46670
                     /locus_tag="LAF92_001142"
     CDS             46341..46670
                     /locus_tag="LAF92_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBI9790187.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            46670..47275
                     /gene="recR"
                     /locus_tag="LAF92_001143"
     CDS             46670..47275
                     /gene="recR"
                     /locus_tag="LAF92_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBI9790188.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTQAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            47385..49259
                     /gene="htpG"
                     /locus_tag="LAF92_001144"
     CDS             47385..49259
                     /gene="htpG"
                     /locus_tag="LAF92_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBI9790189.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            49440..50084
                     /gene="adk"
                     /locus_tag="LAF92_001145"
     CDS             49440..50084
                     /gene="adk"
                     /locus_tag="LAF92_001145"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBI9790190.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            50320..51282
                     /gene="hemH"
                     /locus_tag="LAF92_001146"
     CDS             50320..51282
                     /gene="hemH"
                     /locus_tag="LAF92_001146"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBI9790191.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(51279..>51504)
                     /locus_tag="LAF92_001147"
     CDS             complement(51279..>51504)
                     /locus_tag="LAF92_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HBI9790192.1"
                     /translation="DLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPG
                     TLHAFLHYSRMMKTADEALRDGAQFFTAQL"
BASE COUNT        12834 a        12847 c        13730 g        12093 t
ORIGIN      
        1 tcatcatgtt gtagttgagg ttccacataa tccagatgga gcctacaacc aggatagcga
       61 tgattagcac ggtgaagaca aacgccgtca tgttccagcc ttcatctgat ttggtattca
      121 tgtgcaggaa gcacaccaga tgcaccagaa cctgtaccac tgccattgcc aggattgttc
      181 ccagaattac ggccggagag gcagcccctg tcatcaccat ccagaacgga atcaccgtca
      241 gaatgatcga caggataaag cctgtcatgt aggtttttac gctgccatgg gacgcgccgc
      301 tgtgatcggt agaatgactc attacatcgc ccccatcaga taaacaacag tgaacacaca
      361 gatccaaacc acatccagga agtgccagaa caggctcagg cacatgatgc gggtacggtt
      421 agtgctggtc aggccgcgac gagcgatttg taccatcagc accgccatcc agataagacc
      481 ggaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag aacgctgaca ggaagccgct
      541 gcgatccgga cccatgccgt taacaatcag gtgatggaat tcgtagattt ccatcccgat
      601 aaatccggca ccaaacaacc aggtcaacgc cagccaggag ataacctggc ttttgttgtt
      661 tttgtacatg gcgatagccg ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt
      721 ttcaaccaga acgaacggca gttcgaaaat gtccttacct gtcgggccgc ctgcggtgcc
      781 gttcaccaga acggcatagg tagcaaacaa gattgagaac agaatgcagt cgctcatcag
      841 gtagatccaa aatccgaaga ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc
      901 gtgggcagtc gcgtgcgtca aagtatcagt tgccattttt cagccctgcc ttagtaatct
      961 catcgaaatg ctggttttcc agtttttcga tttctgccac cggcacgtag taatccacgt
     1021 cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc tgcgaagcca acaatcgcca
     1081 gccaccagat atgccagatc atggcgaaac cgaagatggt ggagaaagct gcaatgacga
     1141 tacctgcacc gctgtttttc ggcatatgaa tttcttcata gtggtcaggc tttttatacg
     1201 cttcgccttt ctctttcatt tcccagaatg catcacgctc gtgaacgtgc ggcacaacgg
     1261 caaagttata gaacggaggc ggggaagagg ttgcccactc cagcgtacgg ccaccccacg
     1321 ggtcgccagt caggtcacgg ttctggtcgc ggtcgcgaat agaaacgtac atctgaataa
     1381 cgaggcagag aatacccagc gcaatcagta ctgcaccgct agctgcaatc atcagcatgg
     1441 tgtggaactg cgggtcaatc tgctggctca aacgacgggt catgcccatg aagcccagcg
     1501 catacagcgg cataaaggca acgaagaagc cgatgatcca gaaccagaac gcgcgtttac
     1561 cccaggtttc attcagtttg aaaccgaagg ctttaggcca ccagtaggtc atccctgcga
     1621 agcagccgaa gaccacgccg ccgatgatca cgttatggaa gtgcgcaatc aggaacaggc
     1681 tgttatgcag aacgaagtcc gcgcccggta ctgccagcag cacgccagtc atcccgccca
     1741 ccgagaaagt gacgataaaa ccgatggtcc acagcatcgc agaatggaac acgatgcggc
     1801 cctgatacat ggtgaacagc cagttgaaga tcttcacccc ggtcgggatg gcgataatca
     1861 ttgtggtgat accaaagaag gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt
     1921 gcagccaaac gatgaacgac agcacggtga tacagacggt tgcccatacc agcgaggtat
     1981 aaccaaacag acgtttacgc gagaaggttg ccgcaatttc ggagaacaca ccgaaaacag
     2041 gcaggatcag gatgtaaact tccgggtggc cccaggccca aatcaggttg atgtacatca
     2101 tcatgttgcc acccatatcg ttggtaaaga aatgggtgcc cagatagcga tccagggtca
     2161 acaacgcgac ggtaaccgtc agaattggga aggaagcaat aatcagtacg ttcgcgcaca
     2221 gtgatgccca ggtaaatact ggcatcttga acatggtcat gcccggtgcg cgcatcttca
     2281 gaatggtaac gaagaagttg ataccggtaa gcgtcgtacc tataccggat agctggagac
     2341 tccatatcca gtaatcgaca ccgactcccg gactgtactc tattcccgat agcggtggat
     2401 aggccagcca gccggtctgc gcaaattcgc ccacgccgag agaaacgtta accagaatca
     2461 caccaacaac agtaaaccag aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg
     2521 cgccgatctg cagcggaacc accaggttca tcagaccgat aacgaaaggc atcgccacga
     2581 agaagatcat aatcacgccg tgcgcggtga agatctgatc gtagtggtga ggtggcagga
     2641 agcccgcttc acccgccgag gcaagagcct gctggctacg catcataatg gcgtcagcaa
     2701 aaccacgcag caacatcaca atcgccacga tgatatacat gataccgagg cgtttatggt
     2761 cgacggaggt cagccactct ttccacaggt aggtccactt accgaagtaa gtgatcaggc
     2821 caacgagcgc cagacctccc aaaataatgc cagcgatcgt aaccatgacg ataggttcat
     2881 ggaacgggac tgcatcaagt gataattttc cgaacatctt tattcttcct caaccccttt
     2941 aatgggcgga ttccgcgtgg ctcatgtcca tgccttccat accttcgtgt gcactgtgct
     3001 caccttctgg ctgggtcatg tccatgctct taccgtgagc cataaacttg ttaatcacat
     3061 cggcaaacaa gtctggtttc acgttggaga aatattccac ctggttgtat tcgctaggcg
     3121 cggccagttt ttcgaacgca gccatgtcag acatggtgtt cggcgactgt ttcgcttttg
     3181 cgacccattg gtcgaattcg gcgcgatccg gtgttgcaat agctttgaac ttcatgcctg
     3241 agaagcccgg cccgctgtag ctggcggaga taccgtcata agtaccgggt tcgttggcga
     3301 tcagatgcag gcgagtctgc ataccggcca tggcataaat ctggctaccc agacgcggaa
     3361 tgaagaagga gttcatcacg gagttggagg tcactttgaa gtacaccgga gtgttcgccg
     3421 ggaaagcgat ttcattcacg gtagcaatgc cctgttccgg gtagatgaag aaccatttcc
     3481 agtccatgga aaccacttcg atggtaatgg gcttctcgtc gtgtgccagc ggcttgctag
     3541 gctcaagagc gtgagtggtt ttccaggtca gtaccgcaag gaagatgatg attaagatag
     3601 gtaccgtcca gaccacagct tccactttat tggagtgtga ccagttcggg ctgtacttag
     3661 catctttatt gctcgcacga tacttccagg cgaaaccaac agccatcaag attgcgggaa
     3721 taacgacaat caacatcagg ccaaatgccg tcagtatcag tgaacgttgc tccagaccaa
     3781 tctgtccttt gggatctaac agcgcagaat tacagccact gagcaataca gtgcctgcaa
     3841 ataatgacaa ccatcccaaa cttttattgt atttcctgag tctcatttaa cgacctcaat
     3901 tccacgggat ctggtggcgt ttaaagtgtg tgggcatttt acgggaaggt tacattactg
     3961 taaacatgat taaattcctg ttacttggat ttggctgtca ggtcacatat gttgcaaaac
     4021 atatcaagtt gattttaaga agctgttgca aaagggcgtt ataacgaaag gggagtgcag
     4081 aaggagtttg gacgggcaat tggtataacc aatctggaat aaaacaattg tttaacaaat
     4141 aattatcatt atggtgacaa ttgggtgggg gaaaataaag actcattagc tgaatcgtgt
     4201 aagaaaaaat agaattcata aattgcgtca ataatttcca aacgctaagc cgcacaaaag
     4261 aacaaatatt attaagcgat actgatgttt atttagccgt aataattacg gcgagtgatt
     4321 actacagcta aataatattt acagattacg tcagatgcgt ttttcgtagc gccagataat
     4381 ccagcaaacc accaagcacg acgccagaaa gggcgactaa aaccccgact tccagcagag
     4441 caggcaggaa agagaagtgc gtcaaatcca gcgcgtccat cgtcaacagc agtaaccaca
     4501 cggccaacag gctgacgccc gccgccagtg tccacatggc aaaggcataa cctgccggat
     4561 attcggtacg ggagataaag ttgtcattta ctcgtgtata ttcaagcgtc tggcggcaaa
     4621 ccagcagcaa aataagcccc ggtacggcag cggcgacgga gaatagatag aatgtcgacc
     4681 agccgtgtgc ttcaacaaac caacccgcca cggggccgac ataaactcgc cctacagcgg
     4741 aaagcgctga gagcagggca aattgagttg cggaaaatga cttattacac agcgtcatta
     4801 acagcgcgac aaaggctgat gtgcccatcc cgccacagag gttttcgaaa aagacggctg
     4861 cgcccatgct gtagagatgc ttatcagtaa tcgacagcag ccagtaacca gcgttagacg
     4921 caccttgtaa aatgccgaaa atcagcagcg cccggaacag cgacaggcgc tgcatcaaaa
     4981 tcccaccgta caatgcgccc acaatggtcg ccagtaagcc aagcgttttg ttaaccacgc
     5041 ctacttcacc cgcatcaaac ccgacgccgc gaatcaaaaa cgtggttgtc aggctcatgg
     5101 cgaatgcgtc gcccagctta tacagcacga taagaagcaa aataagccag gcattattgc
     5161 gaccaaagaa atctcgcaga ggtgcaacaa ccgcttgttc cagcgttttt ggcacaggaa
     5221 tggtgtcggt cggttctggt gcaagcaacg tcgcgataat acaagggatc aacagcgccg
     5281 ccatcaacca gtacatgccc tgccagccca gccatttatc tgccagccac agggctaagc
     5341 cgccggaaac cagcatccct aaacggtaac ccagcacgct gattgccgcg cccgcaccac
     5401 gttcttctgc cgggagcaca tcggttttcc acgcatcgaa gacaatatcc tgggaggccg
     5461 agcaaaaagc gatcaccacc gccagtgccg ccatccagcg gagttgggtg ccaggttcga
     5521 gaaaacccat cgccgcaatg gcgactaata acaggatttg cgtggcgagc agccaaccgc
     5581 gccgccgccc aaaaaatggg ggcgtgtagc ggtccatcag cggtgaccag aggaatttaa
     5641 aaacgtaagc ctggcctacc agagagaaga aaccaatggt tttgagatca atattctcga
     5701 ccgtcatcca ggcctgtaag gtgccggatg tcagggcgag cggtagcccg gaagcaaaac
     5761 ccaggatcag caatatggct gaacgcggct gttgaaaaat acgtaaatat tgactggaca
     5821 tgggcgtcta caggcccggt ttgcaccggg ccagagggca gaaattaacg cgcgttctgc
     5881 ttgataaatt cgtgaatgct ggtgtcctga gacatatcag cgatggtatc ggtcagcaca
     5941 ctgttaaccg catcggcgat atttttgttg gaggcctgga acgcaccttc aacgttgtag
     6001 ctggcacgat agtttttggt cattttgttg ccattctgcg cggtagcgat gatggcgata
     6061 tccgctttgg tagcgatgtt gtagcgcacg ttgccctggg acacgtcagc atacagttgg
     6121 ctaacgatga tttgcagatt aaccgggcca ttcggaccaa ccatgtaacc acgcgcggtc
     6181 atctgttttt ccagcacttc ttgcagcagg aaacgcagat cgcgggaggc ggtcagggta
     6241 acgatttgat tatcgcgggt gacttttgcc agcgcctgat cggtacgctg atcggcacca
     6301 ttaatgctta cggtgacgcc catcaggctt ggatcctgct gtggcagtgt aatcgtcggg
     6361 gaaacttcaa tagttgttgg cggttttgcg catcctgcaa gcataaacag agcaactaac
     6421 gggaagagga tttttttgaa catgttcggg ctctcagaga ctcttaagcg tgtttggtaa
     6481 aaattcccgc catcataaca ttgccaacgg cgaggggaag tgggtaaggc atgtaaattc
     6541 atcatgttga cgaaataatc gcccctggca aaagaaacac tgatgcgagg cctgtgtttc
     6601 aatctttaaa tcagtaaact tcatacgctt gacggaaaaa ccaggacgaa acctaaatat
     6661 ttgttgttaa gctgcaatgg aaacggtaaa agcggctagt atttaaaggg atggatgaca
     6721 tctcagcgtt gtcggaggag atatttcatg atgatacgtg agcggataga agaaaaatta
     6781 agggcggcgt tccaacccgt attcctcgaa gtagtggatg aaagctatcg tcacaatgtc
     6841 ccagccggct ctgaaagcca ttttaaagtt gtgctggtca gcgatcgttt tacgggtgaa
     6901 cgttttctga atcgtcatcg aatgatttac agtactttag cggaggaact ctctactacc
     6961 gttcatgcgc tggctctgca tacttacact attaaggagt gggaagggtt gcaggacacc
     7021 gtctttgcct ctcctccctg tcgtggagca ggaagcatcg cgtaaaaacg catttgcaac
     7081 tgtcggcgct tttccagtat gttgctaaag attttatgaa aaacggcctg cgggccgttt
     7141 tgttttgtct ggattttgcg ctttttgccc agcattcaga cgaaaattgc ccgggaattg
     7201 tgaaaaaata cgcgacagcg cgcaataacc gttctcgact cataaaagtg atgccgctat
     7261 aatgccgcgt cctatttgaa tgctttcggg atgattctgg taacagggaa tgtgattgat
     7321 tataagaaca tcccggttcc gcgaagccaa caacctgtgc ttgcggggta agagttgacc
     7381 gagcactgtg attttttgag gtaacaagat gcaagtttca gttgaaacca ctcaaggcct
     7441 tggccgccgt gtaacgatta ctatcgctgc tgacagcatc gagaccgctg ttaaaagcga
     7501 gctggtcaac gttgcgaaaa aagtacgtat tgacggcttc cgcaaaggca aagtgccaat
     7561 gaatatcgtt gctcagcgtt atggcgcgtc tgtacgccag gacgttctgg gtgacctgat
     7621 gagccgtaac ttcattgacg ccatcattaa agaaaaaatc aatccggctg gcgcaccgac
     7681 ttatgttccg ggcgaataca agctgggtga agacttcact tactctgtag agtttgaagt
     7741 ttatccggaa gttgaactgc agggtctgga agcgatcgaa gttgaaaaac cgatcgttga
     7801 agtgaccgac gctgacgttg acggcatgct ggatactctg cgtaaacagc aggcgacctg
     7861 gaaagaaaaa gacggcgctg ttgaagcaga agaccgcgta accatcgact tcaccggttc
     7921 tgtagacggc gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg cgatgggcca
     7981 gggtcgtatg atcccgggct ttgaagacgg tatcaaaggc cacaaagctg gcgaagagtt
     8041 caccatcgac gtgaccttcc cggaagaata ccacgcagaa aacctgaaag gtaaagcagc
     8101 gaaattcgct atcaacctga agaaagttga agagcgtgaa ctgccggaac tgactgcaga
     8161 attcatcaaa cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg ctgaagtgcg
     8221 taaaaacatg gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt ctcaggcgat
     8281 cgaaggtctg gtaaaagcta acgacatcga cgtaccggct gcgctgattg acagcgaaat
     8341 cgacgttctg cgtcgtcagg ctgcacagcg tttcggtggc aacgaaaaac aagctctgga
     8401 actgccgcgc gaactgttcg aagaacaggc taaacgccgc gtagttgttg gcctgctgct
     8461 gggcgaagtt atccgcacca acgagctgaa agctgacgaa gagcgcgtga aaggcctgat
     8521 cgaagagatg gcttctgcgt acgaagatcc gaaagaagtt atcgagttct acagcaaaaa
     8581 caaagaactg atggacaaca tgcgcaatgt tgctctggaa gaacaggctg ttgaagctgt
     8641 actggcgaaa gcgaaagtga ctgaaaaaga aaccactttc aacgagctga tgaaccagca
     8701 ggcgtaattt acgcagcata acgcgctaaa ttcgcacaaa ggcccgtcac cgccaggtgg
     8761 tgggcttttt tttgtcatga attttgcatg gaaccgtgcg aaaagcctct ttcggtgtta
     8821 gcgtaacaac aaaagattgt tatgcttgaa atatggtgat gccgtaccca taacacaggg
     8881 actagctgat aatccgtcca taaggttaca atcggtacag caggtttttt caattttatc
     8941 caggagacgg aaatgtcata cagcggcgaa cgagataact ttgcacccca tatggcgctg
     9001 gtgccgatgg tcattgaaca gacctcacgc ggtgagcgct cttttgatat ctattctcgt
     9061 ctacttaagg aacgcgtcat ttttctgact ggccaggttg aagaccacat ggctaacctg
     9121 attgtggcgc agatgctgtt cctggaagcg gaaaacccag aaaaagatat ctatctgtac
     9181 attaactccc caggcggggt gatcactgcc gggatgtcta tctatgacac catgcagttt
     9241 atcaagcctg atgtcagcac catctgtatg ggccaggcgg cctcgatggg cgctttcttg
     9301 ttgaccgcag gggcaaaagg taaacgtttt tgcctgccga attcgcgcgt gatgattcac
     9361 caaccgttgg gcggctacca gggccaggcg accgatatcg aaattcatgc ccgtgaaatt
     9421 ctgaaagtta aagggcgcat gaatgaactt atggcgcttc atacgggtca atcattagaa
     9481 cagattgaac gtgataccga gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac
     9541 ggtctggtcg attcgattct gacccatcgt aattgatgcc agaggcgcaa ctgtgccgct
     9601 atacttatcc agggcggcac aacgctgtaa gcggcttgcg cctgagaatg gcatttgcgt
     9661 cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc catgacagat aaacgcaaag
     9721 atggctcagg caaattgctg tattgctctt tttgcggcaa aagccagcat gaagtgcgca
     9781 agctgattgc cggtccatcc gtgtatatct gcgacgaatg tgttgattta tgtaacgaca
     9841 tcattcgcga agagattaaa gaagttgcac cgcatcgtga acgcagtgcg ctaccgacgc
     9901 cgcatgaaat tcgcaaccac ctggacgatt acgttatcgg ccaggaacag gcgaaaaaag
     9961 tgctggcggt cgcggtatac aaccattaca aacgtctgcg caacggcgat accagcaatg
    10021 gtgtcgagtt gggcaaaagt aacattctgc tgatcggtcc gaccggttcc ggtaaaacgc
    10081 tgctggctga aacgctggcg cgcctgctgg acgtcccgtt caccatggcc gacgcaacca
    10141 cgctgaccga agccggttat gtgggcgaag acgttgaaaa catcattcag aagctgttgc
    10201 agaagtgcga ttacgacgta cagaaagcgc agcgcgggat tgtctacatc gatgaaatcg
    10261 acaagatttc tcgtaagtca gacaacccgt ccattacccg agacgtttcc ggtgaaggcg
    10321 tacagcaggc actgttgaaa ctgatcgaag gtacggtagc tgctgttcca ccgcaaggtg
    10381 ggcgtaaaca tccgcagcag gaattcttgc aggttgatac ctctaagatc ctgtttattt
    10441 gtggcggtgc gtttgccggt ctggataaag tgatttccca ccgtgtagaa accggctccg
    10501 gcattggttt tggcgcgacg gtaaaagcga agtccgacaa agcaagcgaa ggcgagctgc
    10561 tggcgcaggt tgaaccggaa gatctgatca agtttggtct tatccctgag tttattggtc
    10621 gtctgccggt tgtcgcaacg ttgaatgaac tgagcgaaga agctctgatt cagatcctca
    10681 aagagccgaa aaacgccctg accaagcagt atcaggcgct gtttaatttg gaaggcgtgg
    10741 atctggaatt ccgtgacgag gcgctggatg ctatcgctaa gaaagcgatg gcgcgtaaaa
    10801 ccggtgcccg tggcctgcgt tccatcgtag aagccgcact gctcgatacc atgtacgatc
    10861 tgccgtccat ggaagatgtc gaaaaagtgg ttatcgacga gtcggtaatt gatggtcaaa
    10921 gcaaaccgtt gctgatttat ggtaagccgg aagcgcaaca ggcatctggt gaataattaa
    10981 ccattcccat acaattagtt aaccaaaaag gggggatttt atctcccctt taatttttcc
    11041 tctattctcg gcgttgaatg tgggggaaac atccccatat actgacgtac atgttaatag
    11101 atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg aaattaaact aagagagagc
    11161 tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg tattgccgct gcgcgatgtg
    11221 gtggtttatc cgcacatggt catcccctta tttgtcgggc gggaaaaatc tatccgttgt
    11281 ctggaagcgg cgatggacca tgataaaaaa attatgctgg tcgcgcagaa agaagcttca
    11341 acggatgagc cgggtgtaaa cgatcttttc accgtcggga ccgtggcctc tatattgcag
    11401 atgctgaaac tgcctgacgg caccgtcaaa gtgctggtcg aggggttaca gcgcgcgcgt
    11461 atttctgcgc tctctgacaa tggcgaacac ttttctgcga aggcggagta tctggagtcg
    11521 ccgaccattg atgagcggga acaggaagtg ctggtgcgta ctgcaatcag ccagttcgaa
    11581 ggctacatca agctgaacaa aaaaatccca ccagaagtgc tgacgtcgct gaatagcatc
    11641 gacgatccgg cgcgtctggc ggataccatt gctgcacata tgccgctgaa actggctgac
    11701 aaacagtccg ttctggagat gtccgacgtt aacgaacgtc tggaatatct gatggcaatg
    11761 atggaatcgg aaatcgatct gctgcaggtt gagaaacgca ttcgcaaccg cgttaaaaag
    11821 cagatggaga aatcccagcg tgagtactat ctgaacgagc aaatgaaagc tattcagaaa
    11881 gaactcggtg aaatggacga cgcgccggac gaaaacgaag ccctgaagcg caaaatcgac
    11941 gcggcgaaga tgccgaaaga ggcaaaagag aaagcggaag cagagttgca gaagctgaaa
    12001 atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg gttatatcga ctggatggta
    12061 caggtaccgt ggaatgcgcg cagcaaggtc aaaaaagacc tgcgtcaggc gcaggaaatc
    12121 cttgataccg accattatgg tctggagcgc gtgaaagatc gcatccttga gtaccttgcg
    12181 gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct gcctggtagg gccgccgggg
    12241 gtaggtaaaa cctctcttgg tcagtccatt gccaaagcca ccgggcgtaa atatgtccgt
    12301 atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg gtcaccgccg tacttacatc
    12361 ggttctatgc cgggtaaact gatccagaaa atggcgaaag tgggcgtgaa aaacccgctg
    12421 ttcctgctcg atgagatcga caaaatgtct tctgacatgc gaggcgatcc ggcctctgca
    12481 ctgcttgaag tgctggatcc agagcagaac gtagcgttca gcgaccacta cctggaagtg
    12541 gattacgatc tcagcgacgt gatgtttgtc gcgacgtcga actccatgaa cattccggca
    12601 ccgctgctgg atcgtatgga agtgattcgc ctctccggtt ataccgaaga tgaaaaactg
    12661 aacatcgcca aacgtcacct gctgccgaag cagattgaac gtaatgcact gaaaaaaggt
    12721 gagctgaccg tcgacgatag cgccattatc ggcattattc gttactacac ccgtgaggcg
    12781 ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgcc gcaaagcggt taagcagtta
    12841 ctgctcgata agtcattaaa acatatcgaa attaacggcg ataacctgca tgactacctc
    12901 ggtgttcagc gtttcgacta tggtcgcgct gataacgaaa accgtgtcgg tcaggtaacc
    12961 ggtctggcgt ggacggaagt gggcggtgac ttgctgacca ttgaaaccgc atgtgttccg
    13021 ggtaaaggca aactgaccta taccggttcg ctcggcgaag tgatgcagga gtctattcag
    13081 gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg ggatcaaccc tgatttttac
    13141 gaaaaacgtg acatccacgt ccacgttccg gaaggtgcga cgccgaaaga tggtccgagt
    13201 gccggtattg ctatgtgcac cgcgctggtt tcttgcctga ccggtaaccc ggttcgtgcc
    13261 gatgtggcaa tgaccggtga gatcactctg cgtggtcagg tactgccgat cggtggtttg
    13321 aaagaaaaac tcctggcagc gcatcgcggc gggattaaaa cagtgctaat tccgttcgaa
    13381 aataaacgcg atctggaaga gattcctgac aacgtaattg ccgatctgga cattcatcct
    13441 gtgaagcgca ttgaggaagt tctgactctg gcgctacaaa atgaaccgtc tggtatgcag
    13501 gttgtgactg caaaatagtg acctcgcgca aaatgcacta ataaaaacag ggctggcagg
    13561 ctaattcggg cttgccagcc tttttttgtc tcgctaagtt agatggcgga tcgggcttgc
    13621 ccttattaag gggtgttgta aggggatggc tggcctgata taactgctgc gcgttcgtac
    13681 cttgaaggat tcaagtgcga tataaattat aaagaggaag agaagagtga ataaatctca
    13741 attgatcgac aagattgctg caggggctga tatctctaaa gctgcggctg gccgtgcgtt
    13801 agatgctatt attgcttccg taactgaatc tctgaaagaa ggggatgatg tagcactggt
    13861 aggttttggt acttttgccg ttaaagagcg tgctgcccgt actggccgca acccgcagac
    13921 cggtaaagag atcaccatcg ctgctgctaa agtaccgagc ttccgtgcag gtaaagcact
    13981 gaaagacgcg gtaaactaag cgttgtcccc ggtggggatg tgacgaagtt caagggcgca
    14041 tctactgatg tgcctttttt atttgtattc ggtgactttc tgcgtcttgt gggctgacaa
    14101 ttgcccccgt ttcttgtcac aataggcctt tgcgcgcatc gatacgttgc gtgaggtaca
    14161 cagtcatcta cagcggagtg ttgttacacc atgatggaca gcttacgcac ggctgcaaac
    14221 agtctcgtgc tcaagattat tttcggtatc attatcgtgt cgttcatatt gaccggcgtg
    14281 agtggttacc tgattggcgg aggcaataac tacgccgcaa aagtgaatga ccaggaaatc
    14341 agccgtgggc agttcgagaa cgcctttaac agcgagcgta atcgcatgca gcaacagctg
    14401 ggcgatcaat actccgagct ggcagcgaat gaaggctata tgaaaaccct gcgtcaacag
    14461 gtgctgaatc gtctgatcga cgaggcgctg ttggatcagt acgctcgtga gctgaaactg
    14521 ggtatcagtg atgagcaggt taaacaggcg attttcgcga ccccagcctt ccaggttgac
    14581 ggcaaatttg ataacagccg ctataacggt atcctcaacc agatggggat gaccgccgat
    14641 cagtacgccc aggcgctgcg taaccagctc actacccaac agctgattaa cggtgttgcc
    14701 ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg cggcactggt cgcgcaacaa
    14761 cgcgtggtgc gtgaggcgac tatcgatgtt aacgcgctgg cggcgaagca gcctgtgacc
    14821 gaacaggaga ttgccagcta ctacgaacaa aacaaaaaca atttcatgac gccggaacaa
    14881 ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc agcaaccggt tagcgatgcg
    14941 gatatccaga gctactacga tcagcatcag gatcaattca cccagccgca gcgtacccgc
    15001 tacagcatca tccagaccaa aactgaagat gaagcgaaag cggtacttga tgagctgaat
    15061 aaaggcggtg attttgctgc attagccaaa gaaaaatctg ccgatattat ctctgcccgt
    15121 aacggcggcg atatgggttg gttagaagat gccactatcc ccgacgaact gaaaaatgct
    15181 ggtctgaaag aaaaagggca actgtctggt gtcatcaaat cttcggtcgg tttcctgatt
    15241 gtacgtctgg acgacattca gccagcgaaa gtgaaatcgt tagacgaagt acgtgacgac
    15301 attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt actatgcgct gcagcagaaa
    15361 gtgagcgatg cggcaagcaa cgacacggag tctctggccg gtgcagagca agctgccggc
    15421 gttaaagcca ctcagacggg ttggtttagc aaagataacc tgccggaaga gttgaacttc
    15481 aagccggttg ccgacgctat ctttaacggc ggtctggtag gtgaaaacgg cgcgccgggc
    15541 atcaactctg acatcatcac cgtagacggc gaccgcgcat tcgtgctgcg catcagcgag
    15601 cacaaaccgg aagcggtgaa accgttggca gatgttcagg aacaagttaa ggcactggtt
    15661 cagcacaaca aagctgaaca acaggcgaaa gtggatgctg agaaactgct ggttgatttg
    15721 aaagccggca aaggtgcgga agctatgcag gctgccggtc tgaaatttgg cgagccgaaa
    15781 atcttaagcc gttccggtcg tgacccgatt agccaggcgg cgtttgcact gccactgcca
    15841 gcgaaagaca aaccgagcta cggtatggcg accgatatgc aaggtaatgt ggttctgctg
    15901 gcgctggatg aagtgaaaca aggttcaatg ccggaagatc agaaaaaagc gatggtgcag
    15961 ggtatcaccc agaacaacgc acaaatcgtc tttgaagctc tgatgagtaa cctgcgtaaa
    16021 gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat aatcctgaag ccgcctcgca
    16081 aaaaaatgct ttgcagctgt aacaagaaaa ggtcgctttc gcggcctttt ccatttctga
    16141 acattgccat ttgtttactg ttttcactgc cgttaaggtg attccactgt taacaaacaa
    16201 ggagaaaaca gtatgaaaca cggaattaaa gcactgctca ttaccctgtc tctggcttgt
    16261 gccggaatgt ctcatagcgc gctggcggca gcttctgtgg cgaaaccgac aacggtagaa
    16321 accaaagcgg aagctcctgc agcacaaagt aaagcagcag taccggcgaa agccagtgac
    16381 gaagaaggca cccgggtcag cattaataat gccagcgcgg aagagctagc ccgcgcgatg
    16441 aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt atcgcgaaga gtacggtccg
    16501 tttaaaacgg tggaggatct aaagcaggtg ccggggatgg gcaattcgct ggtggaacgt
    16561 aatctggcgg tattaaccct gtaattaatt tgcatagtgg caattttttg ccagactgaa
    16621 gaggtcatac cagttatgac ctctgtactt ataacaacaa cgtaaggtta ttgcgctatg
    16681 caaacacaaa tcaaagttcg tggatatcat ctcgacgttt accaacacgt caacaacgcc
    16741 cgctaccttg aatttctcga agaagcccgc tgggatgggt tggaaaatag cgacagtttt
    16801 cagtggatga cggcccataa catcgccttc gtcgtggtca atatcaatat taactatcgt
    16861 cgcccagcgg tattaagcga cctgttaact attaccagtc agttgcagca attaaacggt
    16921 aaaagcggca tcttaagcca ggtcattaca ctggagccgg aagggcaggt ggtagcggat
    16981 gcgcttatta cgtttgtttg tattgatctt aaaacgcaga aagcattagc tctggaaggg
    17041 gaattgcgcg aaaagctgga gcagatggtt aagtaaacgt tttgtggtgc cggatgctca
    17101 agccgcatcc ggcgacaccc ggaataatta cctcaacccg gttttctgct tcatcgctgc
    17161 catcaccgtc ggtttatcgg ccagataatg attcaaaccg ttggcgcgta aattacatgc
    17221 cgcacaatga ccgcaaccgt cgcctttaat gccgttatag caggtcaacg tttcgttacg
    17281 gactaaatcc agtttgccgt aataatctgc cagcgcccag gtttccgctt tatcaatcca
    17341 catcagcggc gtttcaaaac gaatatcttt cgccatgccc aaactgacgg catggtttag
    17401 tgctttcaca aactcatcgc ggcaatccgg gtagccggag aaatccgttt cacagacgcc
    17461 ggtaattacg gcttctgctt ttacctgata cgcatatatt gccgccagcg tcaggaacaa
    17521 aatattacgc cctgggacaa acgtattcgg gatgccatcg gcttcaggtt cataatcagg
    17581 caccggaatg ctgtcacgtg tcaggctgct gaccgccagc tcgttgagca gcgttacatc
    17641 aagaacctta tgcgcgcgtg ctcccagttt cagcgccagt tcgcgtgcca cgtcgatttc
    17701 tgcgcgatgt cgctgaccgt aatcgaacgt cacgcaatgg acttcatcat attgttgtaa
    17761 tgcctgcacc agacaggtgg tggaatcctg gcctccactg aacacaacga cagcacgttt
    17821 catagataat cctgcttgaa caataaaagc gttatggtaa cgcctgcgat taacccggac
    17881 cagctattca ttgcgacggc gcgggaagcc aggcttcggt aaattcaaac cagccgcgcg
    17941 gtgtcagtcg cacaccgttc acgccgggag gggcactaat gcgatagtga tagttgaaca
    18001 gcggcgtcag cgtcgcgtct gccattaact ggctaaaaac cgctttcagg gcattaaatc
    18061 ggttttcctc atcaggcatt acttgcaccg cgtccagcgt cgattgcaga tgtgcatatg
    18121 ctggagcgtc gaaaacatgt ggccatagtg gatcgcaacg tagccattgc tccagagtat
    18181 attccggtgc ttcgccaatt aatctgtcgc ccatcatgag gtctgcgtgt gccagtaggg
    18241 tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt gagttcacag ccttccgctg
    18301 ccagtgtcgc ctgtaggcgt tctgccatgg tatgaagttc tatcggtaag tgataaacca
    18361 gcgtcaaggt tttcggtagt ttgacttcat ccggtacttg ccagtgcgga atagtccagc
    18421 ctggcagtaa tgcatgactg gcggtgatca ggttttctcc gacttctaac gtttgtaata
    18481 aaccggattg atgaataatg gagatcactt ttcgcgcctg ccagagggag agtcggggac
    18541 ttttgcgcaa cgttaaatag caaaaaccta aactgatgcc actactgacc tggctgaccc
    18601 gttgcagctc ctccggtttg ccgatggtga tttgcacggg atgccgacaa ctggttccca
    18661 aatctttttc gaaaagcggc ggagttatcc agtactcaac cgctttaagc agcggatgac
    18721 gcaaatggta ataatcatgg ctctccaggc gcaccagctc cgctgtgaat tgtgttaagc
    18781 gaaaaggacc agtgccgatc agtgggaatt gcggatgcgc cagatggctg caatagctcg
    18841 ccagccggtg cgcaagccag taatcggggc gatgtaaaaa gaaggtcaga cactgcggat
    18901 gggtgacttc aatacgcttc acgctaataa ataattgatc cagtgctggc agttgtaaca
    18961 gcatcaataa tcgctggtgt aagtgtgagg cttttactgc atcgccgtta tgccagtgta
    19021 gggttgaacg aagataaaag tcccagcgta acccgtcagt agaggtttcc cagtgatgcg
    19081 ctaaatcgcc aatcgggcgc tgagtattat tatcgaagcg ggtcaggccg gaaaatatct
    19141 gcccggcgag atgctgctcg gcacggccgg gcaaaaagcc tggttgtagc ggttcgagcg
    19201 ggcgatagta gggaatacgc aacgtgggtg tatcgttttg ccattgtccg cccataaacg
    19261 gctgtaacag agtgcgcagc tcacctgggg ccagttgcgc cagctccagc acatcttgct
    19321 gctttccggt ttccagtgcc tgttccatca tcgcattgcg tagcgattcc ggcgtgacca
    19381 gaaagcgtaa ttgtccgcgc tttccgcgtc ctgactgcgc ctgccactcc agccatcccg
    19441 cctcctgtgc ctgacgcaac agcgtacgaa catggcgttc gctgcaaaaa cagcgttcgg
    19501 ccagttcgct gacggtgacg gtttgcggct ttccggctga aggttgccac agacgttgat
    19561 actggttaag acggttgagc aatcgcatat aaacccggaa caatattatt taactattca
    19621 ctattacttc cgtatatatc aggtgatact caatcaccat taaccgtgtc acagagtgga
    19681 gaaagaaatg gctcgtctgg cagcatttga tatggatggc actttattga tgcctgacca
    19741 tcatttaggt gagaaaaccc tctctacttt ggcgcgactg cgtgaacgcg acattaccct
    19801 cacttttgcc acggggcgtc atgcgctgga gatgcagcat attctcgggg cgctatcgct
    19861 ggatgcatat ttgattaccg gcaacggaac gcgcgtgcat tctctggaag gtgaactttt
    19921 acatcgtgat gatttacctg cggatgtcgc ggagctggtg ctgtatcagc aatgggatac
    19981 ccgagccagc atgcatatct tcaatgacga cggttggttt accgggaaag agatccctgc
    20041 gttgttgcag gcatttgtct atagcggttt tcgttatcag ataatcgatg tcaaaaaaat
    20101 gccactcggc agcgtcacca agatctgctt ctgtggcgat cacgacgatc ttacacgctt
    20161 gcagatccag ctatacgaag cattaggcga gcgtgcacat ttgtgttttt ccgccacgga
    20221 ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct gcattgacgg tgctgaccca
    20281 acatttaggt ttatcgttgc gcgattgcat ggcctttggt gatgcgatga acgatcgcga
    20341 aatgttaggc agcgtcggta gcggatttat tatgggcaat gcgatgccgc aactgcgcgc
    20401 ggagctcccg catttaccgg tgattggaca ttgccgaaat caggctgtct ctcactattt
    20461 gacgcactgg ctggactatc cacatctacc ttattccccc gaataacgag atcccttcca
    20521 gcaccgggca attgcccggt tttttttgcg ttgagtttgt cattttgtgc catggtgttt
    20581 aaaccgcaca gaataaattg tcgcgatttc acctttaaaa tagaattaaa agagaaaaaa
    20641 attctctgtg gaagggctat gttagataaa attgaccgta agctgctggc cttactacag
    20701 caggattgca ccctctcttt gcaggcactg gctgaagccg ttaatctgac aaccaccccc
    20761 tgctggaagc gcctgaaacg gctggaggac gacggtatcc ttatcggcaa agtcgccctg
    20821 ctggatccgg aaaaaatagg cctcggcctg accgcctttg tgctgataaa aacgcaacat
    20881 cacagcagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg
    20941 gggttctggc gcatggctgg tgaatacgat tatctgatgc gcgtccaggt tgccgacatg
    21001 aaacgctacg acgagtttta taagcgtctg gtaaacagcg tgccggggct gtcggacgtc
    21061 acttccagct tcgcgatgga acagattaaa tacaccactt ctttacccat cgaataaata
    21121 tccagaatca ggtcaggaca caacgcgtgc gattatttgc tcaattaagc tggtatttcc
    21181 gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct tgtcattatc gcgatgctgc
    21241 aactggttcc gccaaaagtg gttggtattg ttgtcgatgg cgtgacagaa caacacttta
    21301 ctaccgggca gatcctgatg tggatcgcca ccatggtgct gattgccgtt gtggtttatc
    21361 tcctgcgtta cgtctggcgc gtattgctgt ttggtgcgtc ttatcaactg gctgttgaac
    21421 tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc tgagttttac ctgcgtcatc
    21481 gcacgggtga cctcatggct cgtgcgacta atgacgtcga tcgcgtcgtg tttgccgccg
    21541 gagaaggggt gctgacgctg gtggattcac tggtaatggg ctgcgctgtg ttgattatga
    21601 tgtctacgca aattagctgg cagttgacct tatttgccct gttaccgatg ccagtgatgg
    21661 cgatcatgat taagcgcaac ggcgatgcct tgcatgaacg ctttaagctg gcacaggcgg
    21721 cgttttctag tcttaatgac cgcacccagg aaagcttcac cagtattcgc atgatcaaag
    21781 cctttggtct ggaagatcgc cagtcggcgt tatttgccgc ggatgccgaa gataccggca
    21841 aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga cccgaccatc tatatcgcga
    21901 ttggtatggc gaacttgctg gcgattggcg gcggtagttg gatggtggtg cagggcagtt
    21961 taacgctggg ccagctcacc agttttatga tgtatttagg tctgatgatt tggccaatgc
    22021 tggcgctggc atggatgttt aacattgtgg aacgtggtag tgctgcgtac agccgtattc
    22081 gcgcgatgct ggcggaagcg ccggtggtga acgatggtag cgaacccgtg ccggaagggc
    22141 gtggcgaact ggatgtaaat attcaccagt tcacgtatcc gcagactgac catcctgcgc
    22201 tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct gggtatttgc gggccgactg
    22261 gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtc agcgaggggg
    22321 atattcgctt tcatgatatt cctctgacga agttacaact cgatagctgg cgtagccgcc
    22381 tggcagtagt tagccagaca ccgttccttt tttccgacac cgtagcgaac aacatcgcgt
    22441 tgggttgccc gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta gccagcgtac
    22501 atgacgatat tttgcgttta ccgcaaggtt acgatacaga ggtgggcgag cgcggcgtga
    22561 tgctttccgg cgggcaaaaa cagcgtatct ctattgctcg tgcgttatta gtcaacgcgg
    22621 aaatcctcat ccttgatgat gcgctttcgg cggtggacgg acgcacagag caccagatcc
    22681 tgcataacct gcgtcagtgg ggacagggta gaacggtaat catcagtgcc catcgccttt
    22741 cggcactgac ggaagccagt gaaattattg tgatgcagca cggacatatc gcccagcgtg
    22801 gcaatcatga tgagctggcg caacaaagcg gctggtatcg cgatatgtat cgctatcaac
    22861 aactggaggc ggcgctcgac gacgctccgg aaattcgcga ggaggccgtc gatgcgtagt
    22921 tttagccaac tgtggccgac tctcaagcgt ctgttagcgt acggttcgcc gtggcgtaaa
    22981 ccgctgggga ttgcggtcct gatgatgtgg gttgcggcag cggcagaagt cagtgggccg
    23041 ctgcttatca gctattttat cgacaatatg gtagcgaaaa ataacctgcc gttgaaaatg
    23101 gttgcagggc tggctgcggc gtatgttggg ctacaactgt ttgccgccgg gctacattac
    23161 gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac
    23221 gtgatggatg ccgcattgcg ccagccatta agcgagtttg atacccaacc cgtcgggcag
    23281 gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg atctctacgt taccgtagtg
    23341 gcaactgtcc tgcgtagcgc cgcactggtg ggcgcgatgc tggtggcaat gttcagcctc
    23401 gactggcgaa tggcgctggt ggcgataatg attttcccgg tagtgctggt ggtaatggtg
    23461 atatatcaac gttacagcac gccgattgtc cgtcgtgtgc gcgcctatct ggcggatatc
    23521 aacgacggct ttaacgaaat catcaatggc atgagcgtta tccagcagtt ccgtcagcag
    23581 gcgcgatttg gcgaacgtat gggtgaggcc agtcgttcac actatatggc gaggatgcaa
    23641 accctgcgcc tcgacggttt tctgctgcgt ccgctgctga gtctgttttc atcgctcatt
    23701 ctttgtggct tgttgatgct gtttggcttc tccgccagcg gcaccattga agtgggcgtg
    23761 ttatatgcgt ttatcagcta tcttgggcga cttaacgaac ccttaatcga actgaccacg
    23821 cagcaggcga tgctgcaaca ggctgttgtt gctggtgagc gcgtgtttga actgatggac
    23881 ggaccgcgcc agcaatatgg caatgatgat cgcccgttac agagcggcac catcgaagtc
    23941 gataacgtgt catttgctta tcgcgatgac aatctggtgc taaagaacat taatctctct
    24001 gtgccttcgc gtaactttgt ggcgctggtc gggcataccg gcagtggcaa aagcaccctc
    24061 gccagtttat tgatgggcta ttacccgcta acggaaggtg agattcgcct tgatggtcgt
    24121 ccattaagtt cgctaagtca cagcgcgctg cgccagggcg tggcaatggt gcagcaagat
    24181 ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc tgggacgtga tatctccgaa
    24241 gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg agctggcgcg cagcatgagt
    24301 gatggtattt atacgccgct gggcgagcag ggaaataatc tctcagtcgg gcaaaagcaa
    24361 ctgctggcac tggcgcgcgt gctggtggag acgccgcaaa tcctgatcct tgatgaggca
    24421 accgccagca ttgactccgg tactgaacag gcgattcaac atgctctggc ggcggtgcgt
    24481 gaacatacca cgctggtggt gattgctcac cgcttatcaa ctattgttga tgccgacacc
    24541 attctggtgc ttcatcgtgg acaagccgtg gagcagggca ctcaccagca actgctggcg
    24601 gcccagggac gctactggca gatgtatcaa ctgcaacttg cgggcgaaga gctggcagcc
    24661 agcgtgcgtg aagaggaatc attgagcgcc tgaatagcgc aatatttcat cgttggtgca
    24721 aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat tttcaatctt cttaacttcc
    24781 tgctctcttt ctcatttttc atttctggca caccgcttgc aatatcttct tcgtgtagca
    24841 gaaccattac cgaattctga ccggagggga tctatgaagc tggtgaccgt gataatcaaa
    24901 ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca ttggtattca gggcctgacc
    24961 gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg ccgagctgta ccggggggcg
    25021 gaatacagcg tcaacttcct gccaaaagta aaaattgatg tggcgattgc tgatgaccaa
    25081 ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat tggcgacggc
    25141 aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga agccgacgaa
    25201 gcggcgctgt aatctctggc acacagcaac aggaacgaaa aatgaagata gcgacgataa
    25261 aaactgggct tgcttcactg gcgatgctgc cgggactggt aatggctgca cctgcggtgg
    25321 ccgataaagc cgacaatgcg tttatgatga tttgtactgc gctggtgctg tttatgacta
    25381 ttccggggat tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg ctgtcgatgc
    25441 tgacgcaggt gacggtgacg tttgcactgg tctgtattct ctgggtggtt tacggttact
    25501 cgctggcgtt tggtgagggc aacaacttct tcggcaacat taactggttg atgctaaaaa
    25561 acatcgaact gacggcggtg atgggcagca tttatcagta tatccacgtg gcgtttcagg
    25621 gctcgtttgc ctgcattacc gtcggcttga tcgttggggc gctggcggaa cgaatccgct
    25681 tctcagctgt gttgattttc gtggtggtat ggctgacgct ctcttacatt ccgattgcgc
    25741 atatggtgtg gggcggtggt ttgctggctt ctcacggtgc gctggatttc gcgggtggca
    25801 cggtggtgca tattaacgcc gcaatcgccg gtctggtggg cgcgtatctg ataggcaaac
    25861 gcgtgggctt cggcaaagag gcgtttaaac cgcacaacct gccaatggtc ttcaccggta
    25921 ctgccattct ctatattggt tggtttggct ttaacgccgg gtcagcgggc acggcgaatg
    25981 aaatcgcggc actggcattt gtgaatactg tggtcgcaac ggcggcggca attcttggct
    26041 ggatcttcgg tgaatgggcg ctgcgtggta agccttctct gctgggagcg tgttctggcg
    26101 cgattgccgg tctggtcggc gtgacgccag cgtgtggcta cattggggtt ggcggcgcgt
    26161 tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg cgttactatg ctcaaacgct
    26221 tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt ggcattgtcg
    26281 gctgtatcat gactgggatt tttgccgcca gttcgctggg tggcgtgggc ttcgctgaag
    26341 gtgtgacgat gggccatcag ttgctggtac agctggaaag catcgccatt acgatcgtct
    26401 ggtccggtgt ggtggcattt attggctaca aactggcgga tctgacggtt ggtctgcgtg
    26461 taccggaaga gcaggagcga gaagggctgg atgtcaacag tcacggcgag aatgcctata
    26521 acgcgtaaca agcactgcaa aaaatagccg gacggtttta cctccggcta tttttttaat
    26581 tgtgattacg catcacccct tcctgaacgg tcgaggcaac cagcacgccg tcctgggtat
    26641 aaaactcacc gcgcacaaag ccgcgtgcgc tggacgccga ggtgctctcc acgctataca
    26701 gcagccattc attcaaatta aacgggcgat ggaaccacat ggaatggtca atggtggcaa
    26761 tctgaatccc cggttcgaga aaaccgatgc cgtgcggctg tagagctacc ggcaggaagt
    26821 taagatcaga agcgtaaccg agcagatact gatgaacgcg caggtcatcc ggcacgctac
    26881 catttgcgcg gatccacacc tgacgatgtg gttctgcgac gtgacctttc agtgggttat
    26941 gaaactccac cggacggact tccagcggac gatcgcagat gaatttatct ttaagcactg
    27001 gcggcagcag gtgcgccagc gattgggcga tttgcgtttc cgaaggcagg ccatcaggcg
    27061 ctggcgcgga cggcattgtt ttttgatgtt cgaaacccgc ttctggtgcc tggaaagagg
    27121 ccgtcatata aaaaatcggt ttgccgtttt gaatagcagc aacccggcgg gcgctgaagc
    27181 tgttaccgtc acgcagcgtt tcgacatcat aaataatcgg cttcttacta tcgccagggc
    27241 gaagaaagta gctgtgaaac gaatgtacca gccgctcttc agggacggtc tcttttgcag
    27301 catacaaggc ctgacccacg acctggccgc caaacacctg gcgtaaacct aaatcttcac
    27361 tctggccgcg aaagagtcct tcctcaattt tttccagatt taacaatgtc agtaaatttt
    27421 ttagcgcctg actcatataa ctctccagta acaaagctgc cgcagcaagc caaagtgagt
    27481 tgagtataac gcaaatttgc cactggtccg atgggtgcaa taatctgaat tacgggctaa
    27541 ttacaggcag aaatgcgtga tgtgtgccac acttattgac gttactattt tgttaaccac
    27601 tcttccggcg aggaaagtta gcccgctggt gcattgataa taaggagaaa tgaatgaaac
    27661 tcgtgcacat ggccagtggt ttagcggttg cgattgcgtt ggcggcttgc gcagataaaa
    27721 gcgcggatat tcagacgcca gccccggctg caaatacgtc tatttcagca acacaacaac
    27781 ctgctatcca gcaaccgaat gtctccggta ccgtctggat ccggcagaaa gtcgcactgc
    27841 cgcctgatgc tgtgctgacc gtgacacttt ctgacgcgtc gttagccgat gcaccgtcaa
    27901 aagtgctggc gcagaaagcg gtgcgtaccg aaggtaaaca gtcaccattc agctttgttc
    27961 tgccatttaa cccggcagat gttcagccga acgcgcgtat tctgttgagt gcggcgatta
    28021 ccgtgaatga caaactggta tttatcaccg ataccgttca gccggtgatc aaccagggcg
    28081 gaactaaagc cgacctgaca ttggtgccgg tacagcaaac cgccgtgccg gttcaggcca
    28141 gcggtggcgc aacgactacc gtaccttcga cttcaccaac tcaggtgaat ccgtcttcgg
    28201 cagttccagc tcctacgcaa tattaagcga cgttaaccct ctccgcccgg agagggttag
    28261 tagttccagc gataacgctg caagtcgatt tgcccgcttc ccgataccat cacaccttct
    28321 gccagtaatg cctgtcgctg acgctgtaaa tccggtccgg ttagcgaaat tgtgccgtgg
    28381 cgattaacca cccggtgcca gggtaaggtg ctgccttcag ggagacgctt taacacaccg
    28441 cccacctggc gcgcggcgcg gggcgatccc gccagtttcg ccacatcacc gtaagtggtg
    28501 acatagcctt cgggaatagc ggcgacgatt tgccagacgc gttggggaaa tgaatcttct
    28561 ttttccatct tttcttcctg aggtaatttt tcagcataat ctggaaaaac gccctagtga
    28621 agtcgcattg cgcaagaaac cagcatctgg cacgcgatgg gttgcaatta gccggggcag
    28681 cagtgataat gcgcctgcgc gttggttctc aacgctctca atgggggctc tgttggttct
    28741 cccgcaacgc tactctgttt accaggtcag gtccggaagg aagcagccaa ggcagatgac
    28801 gcgtgtgccg ggatgtagct ggcagggccc ccacccattt ctgcctccca ccgtttcgtc
    28861 aaaaaaaccc aacatggcta aactttaacc acaactgacg tcgcaagaat tgtctggctg
    28921 cgcagtacgc ttcggaggta tgtctgatga agtatgttga tggttttgtg gttgccgttc
    28981 ctgccgataa aaaggatgcc tatcgggaaa tggccgctaa ggctgcgcca ttgtttaaag
    29041 agtttggcgc gcttcgtatt gtcgaatgct gggccagcga tgtaccggat ggcaaagtga
    29101 ccgattttcg tatggcggtg aaagcggaag agaatgaaga ggttgtcttt agctggattg
    29161 aatacccttc aaaagaggtc cgcgacgctg ctaatcaaaa gatgatgtcg gacccacgga
    29221 tgaaagagtt cggcgagtcc atgccgtttg acggcaagcg aatgatctat ggcggattcg
    29281 agtcaatcat cgacgaatag gtagcgtgac gggctgtgcg gcgtgccgtc agcccaaaaa
    29341 ttcacaaatg ttgctcggcc cagcgtaaga aatcttcttt cggtaatgcc ttgctgtaga
    29401 gccagccctg accgtagtgc acgccatgct ggcgtaacca ctcttcttgt ttactggttt
    29461 cgattccctc cgctaccatt ttcagtttca gtgtttttgc catttcgatg atatgcggcg
    29521 tgacattttt atattccagc gcatcaacga aagatttatc gatcttcaga atgtcgacat
    29581 ccagatcctg taaataactt aaacttgaat atcccgtacc aaaatcatca agataaattt
    29641 catggcccgc ctcccggtag cgagaaatta tcggggcgct ggttttcgga tcggcaaact
    29701 cgcgttcagt gagttcaagc gcgatctgtc tgggattaac ctgatagtga ttgatcattt
    29761 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    29821 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    29881 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    29941 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    30001 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    30061 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    30121 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    30181 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    30241 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    30301 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    30361 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    30421 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    30481 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    30541 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    30601 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    30661 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    30721 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    30781 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    30841 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    30901 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    30961 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    31021 ggcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    31081 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    31141 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    31201 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    31261 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    31321 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    31381 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    31441 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    31501 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    31561 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatctacc acaattttgc
    31621 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    31681 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    31741 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    31801 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    31861 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    31921 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    31981 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    32041 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    32101 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    32161 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    32221 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    32281 gatcacatag ggtatgtctt catggcgaaa aagtataaaa ttcttaataa acagccggtt
    32341 atagctccga aagcgaatta tttattagcg aataaatttc catactgagg aagggatctt
    32401 gtcgtacagt ttattcatgg tcaattcggc gaggcggtga tctgcggctg agtaaaatac
    32461 cgccagttca ttatctgata attcgtattt atttttctcg ataacacgct ccagcgtgtc
    32521 aattgtctgg caacgacgta aacgcattaa ataatcggtt ttcgttaaag gtttttcgga
    32581 catacttcta cccatggttg taataattct aacaagataa actcgcagga ttctctttcg
    32641 tcgcattgac aaaacaacgg aatagtcgat tacctgactt ccgccatttc tgaagatcct
    32701 gcatattaat accataacta ctgaacaaca taaaggtgtc atccagatat tcgtcgatct
    32761 gctcaatgag cttattgtct tcattatact taattttgta attaagtgcg aaggtcgcaa
    32821 tatgctcaat cagttcattc aactggaggt tgatcgccga ggttgggtcg ttaacccagc
    32881 catgattgct ttcttcaagg tttgcaaggc agtcatgata cagggtttca cagagaaact
    32941 taagctgtgc gatatcatgt cttttgggtg agtattcatc cataacgcgt ccccttctta
    33001 gcggttgaac taacggacac cttttgggat ggaaaaaact tactgacctg gacttgccct
    33061 ctcgtttgtt agcttaacta taagccactc tttgcaggtt ttcatcgcat tattacgaaa
    33121 aattacaatt aggaaggaat cgggacagga ctcacttttt catactagca ctaacccgca
    33181 gcaggtaaaa gcagtttgct cgaactgttc ataacatttc aggattatcg ccagatgaaa
    33241 ttggtgacac taataccagg attgctctga atatgacgta ataaccgagg aatgaataaa
    33301 gaattaccgc aaataattaa gaatagcctc taaatgatta tggattatta aatctattag
    33361 cgctacttaa tataaattaa ctaacaatca gtatttttta tgaattttat ccgtggttaa
    33421 tactggtttt cgtatgagat cctgagttgg tggttcaatt actccttaat gttcgtaggt
    33481 tatgcataaa aaaggccgct tgcgcggcct tagtgattac acgttgtatc aatgatgatc
    33541 gacagtatgg ctgtgctcga tatcttcatt cttgcggcta aagcggcggc gaaccacaac
    33601 aaagaatacc ggaacgaaga agattgccag taccgttgcg gtcaccatcc cgcccattac
    33661 accggtacct actgcgttct gcgcgccgga accagcacca gtactgataa ccagcggcat
    33721 aacgccgagg ataaacgcca gcgaggtcat caggatcgga cgtaaacgca tccgcactgc
    33781 atcaagcgtc gcttcaatca gacctttacc ttctttatcc atcaagtctt tggcgaattc
    33841 gacgataagt atcgcgttct tcgccgacaa cccaatggtt gtgagcaggc ctacctggaa
    33901 gtaaacgtca ttggtcaggc cacggaaggt ggcagccagc aacgcaccga taacccccag
    33961 cggaacgacc agcataacgg agaacggaat cgaccagctc tcgtacagcg ccgccagaca
    34021 caggaatacg acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc cggagaggcg
    34081 ttcctgatag gacatccccg tccagtcata gccaacaccg gtaggcagtt tgctcgccag
    34141 ttgttccatt agctccattg cttcaccggt acttttaccc ggtgccgcct ggcctaagat
    34201 ttccatggat ggcaggccat tgtaacgttc cagacgcggc gaaccgtact cccaacgaga
    34261 agaggagaat gccgagaacg gcaccatctg accatcagca gcacgaacat accagtcgcc
    34321 gatatcatcc ggcagcatac ggtatttcgc ttctgacatg acgtaaactt tcttcacacg
    34381 accgcggtcg ataaagtcgt tcacatagct accgccccat gcagcgccca gagtggtgtt
    34441 aatgtcgttg atagaaacac ccagcgcctg cgctttttcc tggtcgatat caatcttaaa
    34501 ctgcggggta tcttccagac cgtttggacg tacgctggtc agcatatcag ggtgcttcgc
    34561 tgcttctgca agcagctggt tacgcgcctg agtcagtttt tcgtgaccaa ggccagcctg
    34621 gtcaatcagc tcaaagtcaa agccggttgc agtacccagt tccacgattg cgggcaggtt
    34681 aaaggcgaaa accatcgcat ctttgatttg cgagaaagcg cgtgttgcac gcatggtaat
    34741 cgcttcaact ttgttttctt cgcccggacg atcggcccag tccttcaagg aaacgaacgc
    34801 aataccggta ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga acaccgactc
    34861 aacgttgttc ttttctttgg tcagatagta atgcgttacc tcattgagca ctttctgcgt
    34921 acgttcctgc gttgcacctg ctggcagctg aaccatggtc ataaatacgc cctggtcctc
    34981 atctggcaag aaggagcttg gcagacgcac gaacagatag gccatgccga ccacgatgat
    35041 cagatacagc accaggtaac gccccgtact gcgcagaata ccgcctacgc tgtcggtgta
    35101 gtggtgcgtg ctcttctcga acatgcggtt aaaccagccg aagaagcctt ttttaccttc
    35161 cccgtgatcg cctttggcaa tcggtttcag catggtggca caaagagccg gagtcaggat
    35221 caacgctacc agtaccgaca gcgccattgc tgaaacaatg gtaatagaga actgacgata
    35281 gatagcacca gtagaaccgc caaagaaggc catcggtacg aataccgccg acagtaccat
    35341 cgcgataccg accagagcgc cctgaatctg ccccatcgac ttacgggtag cttcttttgg
    35401 cggcaaacct tcttccgcca taacacgctc cacgttttct accacaacga tagcgtcatc
    35461 caccaacaag ccgatagcga gcaccatccc gaacattgtt agcgtgttta tcgagaagcc
    35521 aaaggcggca aggacggcaa aggtcccgag caataccacc ggtacggcaa tggtcggaat
    35581 caacgtcgcg cggaagttct gcaggaacag atacataacc aggaacacga ggatgatcgc
    35641 ttcgaccagc gttttaacca cttcgtgaat agagattttc acgaacggcg tggtgtcgta
    35701 cgggtaaaca attttcagac ccgacgggaa gaacggttcc atcttcgcca gttcagcacg
    35761 gattgccgca gcggtatcca gcgcgtttgc accggtcgcc agcttgatcc ccagaccgga
    35821 agccggttgg ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca gctcaatctt
    35881 cgccacatca cgcagcagta cgcgggaacc atcttgattc actttcagca ggattttgcc
    35941 gaactcttca gtagaggtca gacgcgtctg agcaataata gaggcgttaa gctgttggcc
    36001 tttcaccggc ggcgtaccac cgagctgacc cgccgcaacc tgggcgttct gcgctttgat
    36061 ggcggtaatg acatcaaccg gcgttagctg gaatttgttc agctcattcg ggttcatcca
    36121 gatacgcatc gcgtactgtg aaccgaacaa ctgaacatca cccacgcccg acgtacggct
    36181 gatggcatct ttcatattcg ccgctacgta gtcagagata tcctcctgcg tcatggtgcc
    36241 atcggtgttg ataacgccga caaccatcag gaagctgctg gatgatttct caacgctcac
    36301 cccttgctgc tgaacttctt gcggcagcaa cggcatcgcc agctgcagtt tgttctgcac
    36361 ctgaacctgc gcgatatccg catcagtacc agactcaaag gtcagggtga tctgcacggt
    36421 acccgtggag tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg
    36481 ttcgataacc tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccgg ggtaggaggc
    36541 ggagatcgtt actgccggcg gtgcaatcgt aggatattgc gccaccggca gtttgaggat
    36601 cgccagcccc cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa tcgggcgatc
    36661 gataaagaaa ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag
    36721 gctgagcacc gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct
    36781 ggacaccagg acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac
    36841 cttctgtcac cagccactta tcgccaatag cctggcttgc aacgatcgga cgggtttcca
    36901 ctttgtcatc cgcgccaact accagtacgg tggcatcacc acgcggcgta cgggttacgc
    36961 cctgttgcgg gactaaaata gcgtttggat taagcccttc ttccagacgt gcgcgcacga
    37021 acatacccgg cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc
    37081 cagtggtctg atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa
    37141 tgccgtcact ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc agcgtgccat
    37201 tcgccagttc ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaacgtaga
    37261 tcggatcaag ttgctgcacg gttgccagcg cagtcgcctg accgttctgt accaatgcgc
    37321 cttccgtcac gttcgactta ccaatgcgac cgctaatcgg agaggtgact ttggtgtaag
    37381 ccagattgat ccgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct
    37441 gttgcgcatc agccagagcc tgatcgtact cttgcttact gatgtactga gtaccgagca
    37501 gtttctgata acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca
    37561 gatcaccttt cgcactgtcg tatgtcgcct gataggtcgc aggatcaatc tgatagagag
    37621 agacacctgc ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa
    37681 cttgaggacg aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga
    37741 tctgcagagg ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt
    37801 gttgggcctg tttgtcgtca catcctgtta gggctaagct gcctgagagc atcagaacga
    37861 ccgccagagg cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca
    37921 aattggtcaa tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta
    37981 tggtacatac attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc
    38041 acgaaaaacc aaacaagaag cgcaagaaac gcgccaacac atcctcgatg tggctctacg
    38101 tcttttctca cagcaggggg tatcatccac ctcgctgggc gagattgcaa aagcagctgg
    38161 cgttacgcgc ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat
    38221 ctgggaactg tcagaatcca atattggtga actagagctt gagtatcagg caaaattccc
    38281 tggcgatcca ctctcagtat taagagagat attaattcat gttcttgaat ccacggtgac
    38341 agaagaacga cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga
    38401 aatggctgtt gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg accgtataga
    38461 acaaacgtta aaacattgta ttgaagcgaa aatgttgcct gcggatttaa tgacgcgtcg
    38521 ggcagcaatt attatgcgcg gctatatttc cggcctgatg gaaaactggc tctttgcccc
    38581 acaatctttt gatcttaaaa aagaagcccg cgattacgtc gccatcttac tggagatgta
    38641 tctcctgtgc cccacgcttc gtaatcctgc cactaacgaa taaccctgaa tctgactcca
    38701 ggatttttcc tggacatttt cgtcgttgct attctggttc actgcgtcgt gatattcttg
    38761 cggtttgact ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc atgactatgt
    38821 tccagtatta caaacgatca cggcattttg ttttttcagc atttattgct tttgtttttg
    38881 tcttgttatg ccagaacacg gcgtttgcgc gggcgtcatc gaatggtgat ctgccgacaa
    38941 aagcggacct gcaggcgcaa cttgactcac taaataaaca aaaagatctt tctgctcagg
    39001 acaaactggt gcagcaggat ctgacagata cattagccac cctcgataaa atcgatcgca
    39061 taaaagaaga gacagttcag ctacggcaaa aagtcgctga agcgccggaa aaaatgcgcc
    39121 aggcgaccgc ggcgttaaca gcacttagcg atgtcgataa cgacgaagaa acgcgcaaaa
    39181 ttctgagcac gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg ctggacgatt
    39241 tgcaaaacgc acaaaacgat ctggcgtctt ataacagcca gctggtttcg ttacagacgc
    39301 agcccgaacg cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa caaattcgca
    39361 gtcgtctgga tgggactgat gtcggcgaga cagccttacg tcccagccag aaagtgttaa
    39421 tgcaggccca gcaggcgttg ctgaatgcgg agattgacca gcagcgtaaa agcctggaag
    39481 ggaacaccgt cttgcaggat accttgcaaa agcaacgtga ttacgtgacg gcgaacagcg
    39541 ctcgtctgga gcaccagtta caactgttgc aagaagcggt aaacagcaag cgcctgactt
    39601 taactgaaaa aacggcgcag gaagccgtct ccccggatga agccgcgcgt attcaggcta
    39661 atccgctggt gaaacaggaa ctggaaatta accagcagtt aagtcagcgt ctgattaccg
    39721 cgactgaaaa cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac tggctggagc
    39781 gggcgctgca atcggaacgc aatattaaag agcagattgc cgtcctgaag ggcagcctgc
    39841 tgttgtctcg tatcctttac cagcaacaac aaacgctgcc ctcggcggat gaactggaaa
    39901 acatgaccaa ccgcatcgcg gatttgcgtc tcgaacagtt tgaagttaac cagcagcgtg
    39961 atgcactttt ccagagcgat gcgttcgtca acaaactgga agaaggtcac accaacgaag
    40021 tcaacagcga agttcacgat gcgttattgc aagtggttga tatgcgtcgc gaattgctgg
    40081 atcaactcaa caaacagttg ggtaaccagc tgatgatggc cattaacctg caaatcaacc
    40141 agcagcagtt aatgagtgtg tcgaaaaacc tgaaatccat cctgactcag caaatctttt
    40201 gggtgaacag taaccgtcca atggactggg actggatcaa agcgttcccg caaagcctga
    40261 aagatgaatt taagtcgatg aaaatcacgg tgaactggga aaaagcctgg cctgccgttt
    40321 ttatcgcttt cctcgccggt ttgccgctgc tgttgattgc cgggctgatc cactggcgtc
    40381 tgggctggct gaaagcgtat caacagaaac tggcttccgc tgtgggttcc ctgcgtaacg
    40441 atagccagct caacacacca aaagcgatcc ttatcgacct gatccgcgcg ctgccggtgt
    40501 gcctgattat tctcgcggtt ggcctgattc tgttgaccat gcagctcaac atcagcgaac
    40561 tgctatggtc gttcagtaaa aaactggcga ttttctggct ggtgtttggc ctgtgctgga
    40621 aggtactgga gaaaaatggc gttgccgtgc gtcacttcgg catgccggag cagcagacca
    40681 gccactggcg tcggcaaatt gtccgcatca gtctcgcgtt gctgcctatc catttctggt
    40741 ctgtggtagc agaactttcc ccgctgcatc tgatggatga tgtgctgggg caggcgatga
    40801 ttttcttcaa cctgctgctg attgccttcc tggtatggcc aatgtgccgc gaaagctggc
    40861 gtgataaaga gtcgcacacc atgcgactgg tcaccattac cgtgctgtcg ataatcccga
    40921 ttgcgctgat ggtgctgact gctacaggct acttctacac tacgctgcgt ctggcaggac
    40981 gctggattga aaccgtttat ctggtgatca tctggaacct gctgtaccag acggtactgc
    41041 gtggcttaag cgtagcggcg cggcgtatcg cctggcgtcg tgcgctggcg cgtcggcaga
    41101 atctggtgaa agagggcgca gaaggtgctg aaccgccgga agaacccacc attgcactgg
    41161 agcaagttaa ccagcagacg ctgcgtatta ccatgttgct gatgtttgcg ctgttcggtg
    41221 tcatgttctg ggcaatttgg tccgatttga tcaccgtgtt cagctatctc gacagcatca
    41281 cgctctggca ttacaacggc actgaagctg gcgctgcggt ggtgaaaaac gtcaccatgg
    41341 gcagtctgtt gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt cgcaacctgc
    41401 ctggtttact ggaagtgctg gtgctctcgc gactgaatat gcgccagggc gcgtcgtatg
    41461 ccattactac catccttaac tacatcatta ttgctgttgg tgcgatgacg gtgttcggat
    41521 cgctgggcgt ctcttgggat aaactccagt ggctggccgc agcattatcc gtaggtcttg
    41581 gttttggttt acaagaaatt ttcggtaact tcgtctccgg tttgatcatt ctattcgaac
    41641 gtccggtgcg tattggcgat acggtaacca ttggtagctt ctcggggacg gtaagtaaga
    41701 tccgtattcg tgcgacaacg attaccgatt tcgatcgcaa agaagtgatc atcccgaaca
    41761 aagcgtttgt taccgagcgt ctgatcaact ggtcgttgac tgacactact acgcgtctgg
    41821 tgatccgtct cggcgtggcc tatggctccg atctggaaaa agtgcgtaaa gtgttactga
    41881 aggcggcgac tgagcaccca agggtgatgc acgaaccaat gccggaagtc ttctttacgg
    41941 cgtttggtgc cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa ctgcgtgacc
    42001 gtagtcgtac tgtcgatgag ctgaaccgta ctatcgatca gctgtgccgt gaaaacgaca
    42061 tcaacattgc ctttaaccag cttgaagtgc atctgcacaa cgagaagggc gatgaggtga
    42121 cggaagtcaa acgcgactac aaaggcgatg acccgacgcc agcggtaggg taataaccaa
    42181 aggtggcgac atttagttgc cccgagatta ctgacaaagt gcgcgttgtt catgccggat
    42241 gcggcgtgac cgccttatcc ggcctacgaa accgcaagaa ttcaatgtat tgcaggagcg
    42301 gtgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgcccaca atcttagggg
    42361 acatttagcg gccccattta tttctcactt ttccgcctca tcatcgcgcg ttaatttctt
    42421 ttcataatcg cgttttacaa tatccagcgc gcgcagcacg gtgcgggctg ggatctgatt
    42481 ttcttccagc agcacaatca aatcgacagc cagtttgaca tcgtcagggg cattttccag
    42541 tgacatactc tctccattgc tagcgggtta agcgcgctag cctgttttcg attttttcca
    42601 gcgcatggcg gcagcgtgcc aggcgcgctt cataggcttc cacttcacga tgcagcgttt
    42661 gctgttccac gagatcggtc acccgcgcca ggcgggctct gcgttcggca accatctcac
    42721 gtagccgccg ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg gcaattttcg
    42781 gtggcgcact gtcccactcg cgcaatgacc aggcggaggc ttcacgcgcg atggcttcca
    42841 gttgtgccgc cagatgttcc gccagccagg ccacttgcgg cagctgttgc tgctcaactg
    42901 catgacgaag cgcagccaga ttatcgcccg cctcatcgag acaagcctgc agtgttgtcg
    42961 cacgggtttg aaaaaggtgt ctgtcaaaac gggcacttag cgtggcaaat tgcgctaccg
    43021 gggcacaacg ctgacgcagc gtagcgagct gtccttccag tttttccagc agcagggcgg
    43081 ttttcacgat aagacctcaa tgaaaatgat aattgttatg ctaaagtagc cactctgtaa
    43141 gctgacaaca ctcaatatgc aacgaatcat tttaatcatc attggctggc tggcggtagt
    43201 gctgggtacg ctgggcgtgg tattaccggt attaccgacg acgccgttta tcctgctggc
    43261 ggcctggtgc tttgcccgtt cttccccgcg ctttcacgcc tggttgctgt accgctcatg
    43321 gtttggcagc tatctacgtt tctggcagaa acatcatgcg atgccgcgcg gcgtcaaacc
    43381 gcgggcgatt ttgcttattt tgctcacgtt tgccatttct ctgtggttcg tccagatgcc
    43441 atgggtgcgc atcatgttgc tggtaattct cgcctgtttg cttttctata tgtggcgaat
    43501 tccggtgatt gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag ttgcaattat
    43561 tgcgtacagc cagtacattc tggcgttttc gagcacaggc gcaggcggtc aaaggttaaa
    43621 caactgttac ttttgatacg tttaaaacgc gccgtgagta ccaccgtaac aagcaggcat
    43681 acacttatga ccgcgactgc acagcagctt gagtatctca aaaatagcat caaaagcatt
    43741 caggactacc caaaacccgg cattcttttc cgcgatgtca ccagcttact ggaagacccg
    43801 aaagcttacg ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc gggcattacc
    43861 aaagttgtcg gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc tctgggtctg
    43921 ggcgttggct ttgtaccggt ccgtaaaccg ggcaaactgc cgcgtgaaac catcagtgaa
    43981 acttacgacc tggaatacgg caccgatcag ctggagatcc acgttgatgc catcaaaccg
    44041 ggcgacaaag ttctggtggt ggacgatctg ctggcaaccg gcggcactat cgaagcgacc
    44101 gttaaactga tccgtcgtct gggtggtgaa gtggctgacg ctgcgttcat cattaatctg
    44161 ttcgatcttg gcggcgaaca gcgcctcgaa aagcagggca ttaccagcta cagccttgtc
    44221 ccgttcccgg gccattaatt ttcgccagcc cacgtcatgg gcagcacaag atgtgcattc
    44281 agcctcgccg ttctgacggg gctgtgttag cattacccct tcgtgaatcc accttccagc
    44341 gtttcagagc ctgccaatga gttatcaggt cttagcccga aaatggcgcc cacaaacctt
    44401 tgctgacgtc gtcggccagg aacatgtgct gaccgcactg gcgaacggct tgtcgttagg
    44461 gcgtattcat catgcttatc ttttttccgg tacccgtggc gtcggaaaaa cctctatcgc
    44521 ccgactgctg gcgaaggggc taaactgcga aaccggcatt accgcgacgc cgtgcggcgt
    44581 gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc gatctgattg aaatcgacgc
    44641 cgcctcgcgc accaaagttg aagatacccg cgacctgctg gataacgtcc agtacgctcc
    44701 ggcgcgtggt cgtttcaaag tttatctcat cgacgaagtg catatgctgt cgcgccacag
    44761 cttcaacgca ctgttaaaaa cccttgaaga gccgccagag cacgttaagt ttctgctggc
    44821 gacgaccgat ccacagaaat tgccggtgac gattttgtcg cgctgtttgc aatttcatct
    44881 caaggcgctg gatgtcgagc aaattcgcca tcaacttgag cacatcctca acgaagaaca
    44941 tatcgctcac gagccgcggg cgttgcaatt gctggcgcgc gccgctgaag gcagcctgcg
    45001 agatgcctta agtctgaccg accaggcgat tgccagcggt gacggccagg tttcaaccca
    45061 ggcagtcagt gcgatgctgg gtacgcttga cgacgatcag gcgctgtcgc tggttgaagc
    45121 gatggtcgag gccaacggcg agcgcgtaat ggcgctgatt aatgaagccg ctgcccgtgg
    45181 tatcgagtgg gaagcgttgc tggtggaaat gctcggcctg ttgcatcgta ttgcgatggt
    45241 acaactttcg cctgctgcac ttggcaacga catggccgcc attgagctgc ggatgcgtga
    45301 actggcgcgc accataccgc cgacggatat tcagctttac tatcagacgc tgttgattgg
    45361 tcgcaaagaa ttaccgtatg cgccggaccg ccgcatgggc gttgagatga cgctgctgcg
    45421 cgcgctggcg ttccatccac gtatgcctct gcctgagcca gaagtgccac gccagtcctt
    45481 tgcgcccgtc gcgccaacgg cagtaatgac gccaacccag gtgccgccgc aaccgcaatc
    45541 agcgccgcag caggcaccga ctgtaccgct cccggaaacc accagccagg tgctggcggc
    45601 gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca aaaaagagtg aaccggcagc
    45661 cgctacccgc gcgcggccgg tgaataacgc tgcgctggaa agactggctt cggtcaccga
    45721 tcgcgttcag gcgcgtccgg tgccatcggc gctggaaaaa gcgccagcca aaaaagaagc
    45781 gtatcgctgg aaggctacca ctccagtgat gcagcaaaaa gaagtggtcg ccacgccgaa
    45841 ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa ctggcggcga agctggcggc
    45901 agaagccatt gagcgcgacg cgtgggcggc acaggtgagt caactttcgc taccaaaact
    45961 ggtcgaacag gtggccttaa atgcctggaa agaggagagc gacaacgcag tatgtctgca
    46021 tttgcgctcc tctcagcggc atttgaacaa ccgcggtgca cagcaaaaac tggctgaagc
    46081 gttgagcacg ttaaaaggtt caacggttga actgactatc gttgaagatg ataatcccgc
    46141 ggtgcgtacg ccgctggagt ggcgtcaggc gatatacgaa gaaaaacttg cgcaggcgcg
    46201 cgagtccatt attgcggata ataatattca gaccctgcgt cggttcttcg atgcggagct
    46261 ggatgaagaa agtatccgcc ccatttgatc gtaagcacag cttacgttcg tcatccttaa
    46321 cgtgattgag agagaaacct atgtttggta aaggcggtct gggtaacctg atgaagcaag
    46381 cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga gatcgcgcag ctggaagtta
    46441 ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa cggtgcacac aactgccgtc
    46501 gcgtagagat cgacccgagc ctgctggaag acgacaaaga gatgctggaa gacctggtgg
    46561 ctgcagcatt caacgacgca gcacgtcgta ttgaagaaac gcagaaagaa aaaatggcct
    46621 ctgtatcctc cggaatgcag ctgccgcctg gctttaagat gccgttctga tgcaaaccag
    46681 cccgctgtta acacagctta tggaagcact gcgctgtctg ccgggcgttg gcccgaagtc
    46741 ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt agcggcggga tgcgtctggc
    46801 gcaggcgctc acccaggcga tgtcggaaat cggccactgc gccgattgcc gcactttcac
    46861 cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt caggaaaacg gtcaaatctg
    46921 tgtggtggag agtccggcgg acatctacgc cattgagcag acggggcagt tttcaggccg
    46981 ttattttgtg ttgatggggc acctgtcacc gctggacggc atcggtccgg atgatatcgg
    47041 ccttgatcgt ctggaacagc gtctggcaga ggaaaaaatc actgaagtga tcctcgccac
    47101 caatcccacg gttgaaggtg aagctaccgc taactacatt gccgagcttt gcgcgcaata
    47161 tgacgtggaa gccagccgaa tcgctcatgg cgtaccggtt ggcggcgaac tggaaatggt
    47221 cgatggcacc acgctgtcac actcccttgc cgggcgtcat aagattcgtt tttaagcaaa
    47281 cgagagcagg atcacctgct ctcgcttgaa attattctcc cttgtcccca tctctccctc
    47341 atcctgtttt taaccttaaa atggcattat tgaggtagac ctacatgaaa ggacaagaaa
    47401 ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct gatgatccat tctctctatt
    47461 ccaataaaga aatcttcctg cgtgagctta tctctaacgc ctccgatgcg gcggacaagc
    47521 tgcgtttccg tgcgctatct aacccggacc tgtacgaagg tgatggcgaa ctgcgcgttc
    47581 gtgtctcttt cgataaagac aagcgcacgc tgaccatctc cgataacggc gtggggatga
    47641 cccgcgacga agtgattgac catctgggga ctatcgctaa atccggtacc aaatcattcc
    47701 tcgaatccct gggttctgac caggcgaaag acagccagct gatcggtcag tttggtgttg
    47761 gtttctactc tgcgtttatc gtggccgaca aagtgaccgt gcgtactcgc gcggcaggcg
    47821 aaaaaccaga aaacggcgtc ttctgggaat cggctggcga aggtgaatat accgttgccg
    47881 acatcaccaa agaagatcgt ggtactgaaa tcaccctgca tctgcgtgaa ggcgaagacg
    47941 agttcctcga tgactggcgc gtgcgttcca tcatcagcaa atactccgac catatcgcgc
    48001 tgccggtaga gatcgaaaaa cgcgaagaga aagacggcga aaccgttatc tcctgggaga
    48061 aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc ggaaatcacc gatgaagagt
    48121 acaaagagtt ctacaaacac atcgcccacg actttaatga tccgctgacc tggagccaca
    48181 accgcgttga aggtaagcag gagtacacca gcctgctgta catcccgtcc caggctccgt
    48241 gggatatgtg gaaccgcgat cataaacacg gcctgaaact gtacgtccag cgtgtgttca
    48301 tcatggacga cgcagaacag ttcatgccga actatctgcg cttcgtgcgt ggtctgattg
    48361 actccagcga tctgccgctg aacgtttccc gtgaaatcct ccaggacagc acggtaacgc
    48421 gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat gctggaaaaa ctggcgaaag
    48481 acgacgcgga aaaataccag accttctggc aacagtttgg cctggtactg aaagaaggtc
    48541 cggcggaaga tttcgctaac caggaagcga tcgccaaact gctgcgtttt gcttctactt
    48601 ataccgattc ttctgcgcag accgtgtctc tggaagacta cgtttcccgc atgaaagaag
    48661 ggcaggagaa aatctactac atcactgcag acagctatgc ggcagcgaag agcagcccgc
    48721 acctggaact gctgcgtaag aaaggcatcg aagttctgct gctttccgac cgcatcgatg
    48781 agtggatgat gaactatctg actgagttcg acggtaaacc gttccagtcg gtgtctaaag
    48841 ttgacgagtc gctggaaaaa ctggctgacg aagttgatga gagcgcgaaa gaagcggaga
    48901 aagcactgac tccgttcatc gaccgtgtga aagccctgct cggcgagcgc gtgaaagatg
    48961 tccgtctgac tcaccgtctg accgatacgc cagcgatcgt ttcgaccgac gcggacgaaa
    49021 tgagcactca gatggcgaaa ctgttcgctg cggcgggcca gaaagtgcca gaagtgaaat
    49081 acatcttcga actgaacccg gatcacgtac tggtgaaacg tgcggcagat actgaagatg
    49141 aagcgaagtt cagcgagtgg gtagaactgc tgctggatca ggcgctgctg gcagaacgcg
    49201 gcacgctgga agatccgaac ctgtttattc gtcgtatgaa ccagctgctg gtttcctgat
    49261 gtcatgccgg atgacctttg tgtcatccgg catttttctt ttcatcatct gcactttccg
    49321 caaattatct tgccattaac cgtttcagcc tcaggtgcct ttcttgaggc aatcgcctgt
    49381 tggtggtatc gtttatcgct ttttcaaaaa attcgacaca ttttaagggg attttcgcaa
    49441 tgcgtatcat tctgcttggc gctccgggcg cggggaaagg gactcaggct cagttcatca
    49501 tggagaaata tggtattccg caaatctcca ctggcgatat gctgcgtgct gcggtcaaat
    49561 ctggctccga gctgggtaaa caagcaaaag acattatgga tgctggcaaa ctggtcaccg
    49621 acgaactggt gatcgcgctg gttaaagagc gcattgctca ggaagactgc cgtaatggtt
    49681 tcctgttgga cggcttcccg cgtaccattc cgcaggcaga cgcgatgaaa gaagcgggca
    49741 tcaatgttga ttacgttctg gaattcgacg taccggacga actgattgtt gatcgtatcg
    49801 taggccgccg cgttcatgcg ccgtctggtc gtgtttatca cgttaaattc aatccgccga
    49861 aagtagaagg caaagacgac gttaccggtg aagaactgac tacccgtaaa gacgatcagg
    49921 aagaaaccgt gcgtaaacgt ctggttgaat accatcagat gactgcaccg ctgatcggct
    49981 actactccaa agaagcggaa gcgggtaaca ccaaatacgc gaaagttgac ggcaccaagc
    50041 cggttgctga agttcgcgct gatctggaaa aaatcctcgg ctaattcgaa agcgcgcacg
    50101 gacagtcccc tcgccccctc ggggagaggg ttagggtgag gggaacaggc ccgcacaagc
    50161 aaacttatca gcaatctcag gccggatatt cattcggcct tttacaaaaa cctcaatctg
    50221 cgctattcct ttttctgatt tgacctctca cagcaattag ttcttcttcc tcacttttcc
    50281 gctacaatta tcaacaagtt gaatcgataa gaggcggtaa tgcgtcagac taaaaccggt
    50341 atcctgctgg caaacctggg tacgcccgat gcccccacac ctgaagcggt aaaacgctat
    50401 ctgaaacaat ttttaagcga cagacgcgtg gttgatacct cacggttgtt atggtggccg
    50461 ttgctgcgcg gcgtgatttt gccgctgcgc tcgccgcgtg tggcgaagct gtacgcctcg
    50521 gtctggatgg aagacggctc gccgctgatg gtttacagcc gccagcaaca gcaggcgttg
    50581 gcacaacgtt taccggatac gcccgtagcg ctgggcatga gctacggctc gccatcactg
    50641 gaaagcgccg tcgatgaact cctggcagag catgtagatc atattgtggt gctgccgctt
    50701 tatccgcaat tctcctgttc tacggtcggt gcggtatggg atgaactggc acgcattctg
    50761 gcgcgcaaac gtagcattcc ggggatatcg tttattcgtg attacgccga taaccacgat
    50821 tacattaatg cactggcgaa cagcgtacgc gcttcttttg ccaaacatgg cgaaccggat
    50881 ctgctgctgc tctcttatca tggcattccc cagcgttatg cagatgaagg cgatgattac
    50941 ccgcaacgtt gccgcacaac gactcgcgaa ctggcttccg cactggggat ggcaccggaa
    51001 aaagtgatga tgacctttca gtcgcgcttt ggccgggaac cctggctgat gccttatacc
    51061 gacgaaacgc tgaaaatgct cggagaaaaa ggcgtaggtc atatacaggt gatgtgcccg
    51121 ggctttgctg cggattgtct ggagacgctg gaagagattg ccgagcaaaa ccgtgaggtc
    51181 ttcctcggtg ccggcgggaa aaaatatgaa tatattccag cgcttaatgc cacgccggaa
    51241 catatcgaaa tgatggctaa tcttgttgcc gcgtatcgct aaagctgagc ggtaaagaac
    51301 tgagcgccgt cgcgaagagc ctcgtcggcg gttttcatca ttcgtgaata atgcaaaaag
    51361 gcatgcagcg tacctgggta gagtttgaac tcacagggct gctgatgcgc cgctaacgtc
    51421 tggtaaagca gacggctgtc atccagcagc ggatcgaact ccgccccggc aataaaacag
    51481 ggcggaactt cgcgagtgag atca
//
LOCUS       DAEVZA010000019        50753 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEVZA010000019 DAEVZA010000000
VERSION     DAEVZA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.019"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..382
                     /gene="kdsB"
                     /locus_tag="LAF92_001148"
     CDS             <1..382
                     /gene="kdsB"
                     /locus_tag="LAF92_001148"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBI9790193.1"
                     /translation="MATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATIPWDRDR
                     FAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYGEKIHVA
                     VAQEVPGTGVDTPEDLERVRAEMR"
     gene            536..1429
                     /locus_tag="LAF92_001149"
     CDS             536..1429
                     /locus_tag="LAF92_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBI9790194.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(1406..2185)
                     /gene="elyC"
                     /locus_tag="LAF92_001150"
     CDS             complement(1406..2185)
                     /gene="elyC"
                     /locus_tag="LAF92_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBI9790195.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPHEQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            2321..3106
                     /gene="cmoM"
                     /locus_tag="LAF92_001151"
     CDS             2321..3106
                     /gene="cmoM"
                     /locus_tag="LAF92_001151"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBI9790196.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            3103..4425
                     /gene="mukF"
                     /locus_tag="LAF92_001152"
     CDS             3103..4425
                     /gene="mukF"
                     /locus_tag="LAF92_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBI9790197.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            4406..5110
                     /gene="mukE"
                     /locus_tag="LAF92_001153"
     CDS             4406..5110
                     /gene="mukE"
                     /locus_tag="LAF92_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBI9790198.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            5110..9570
                     /gene="mukB"
                     /locus_tag="LAF92_001154"
     CDS             5110..9570
                     /gene="mukB"
                     /locus_tag="LAF92_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBI9790199.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            9831..11678
                     /gene="ldtD"
                     /locus_tag="LAF92_001155"
     CDS             9831..11678
                     /gene="ldtD"
                     /locus_tag="LAF92_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBI9790200.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            11859..12407
                     /locus_tag="LAF92_001156"
     CDS             11859..12407
                     /locus_tag="LAF92_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBI9790201.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            12434..13081
                     /gene="gloC"
                     /locus_tag="LAF92_001157"
     CDS             12434..13081
                     /gene="gloC"
                     /locus_tag="LAF92_001157"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBI9790202.1"
                     /translation="MNSRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(13303..14493)
                     /gene="aspC"
                     /locus_tag="LAF92_001158"
     CDS             complement(13303..14493)
                     /gene="aspC"
                     /locus_tag="LAF92_001158"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBI9790203.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(14678..15766)
                     /gene="ompF"
                     /locus_tag="LAF92_001159"
     CDS             complement(14678..15766)
                     /gene="ompF"
                     /locus_tag="LAF92_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBI9790204.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(16368..17768)
                     /gene="asnS"
                     /locus_tag="LAF92_001160"
     CDS             complement(16368..17768)
                     /gene="asnS"
                     /locus_tag="LAF92_001160"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBI9790205.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(17937..19139)
                     /gene="pncB"
                     /locus_tag="LAF92_001161"
     CDS             complement(17937..19139)
                     /gene="pncB"
                     /locus_tag="LAF92_001161"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBI9790206.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            19405..22017
                     /gene="pepN"
                     /locus_tag="LAF92_001162"
     CDS             19405..22017
                     /gene="pepN"
                     /locus_tag="LAF92_001162"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBI9790207.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRCLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(22224..22991)
                     /gene="ssuB"
                     /locus_tag="LAF92_001163"
     CDS             complement(22224..22991)
                     /gene="ssuB"
                     /locus_tag="LAF92_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBI9790208.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(22988..23779)
                     /gene="ssuC"
                     /locus_tag="LAF92_001164"
     CDS             complement(22988..23779)
                     /gene="ssuC"
                     /locus_tag="LAF92_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBI9790209.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSRRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(23790..24935)
                     /gene="ssuD"
                     /locus_tag="LAF92_001165"
     CDS             complement(23790..24935)
                     /gene="ssuD"
                     /locus_tag="LAF92_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBI9790210.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(24932..25891)
                     /gene="ssuA"
                     /locus_tag="LAF92_001166"
     CDS             complement(24932..25891)
                     /gene="ssuA"
                     /locus_tag="LAF92_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBI9790211.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(25884..26459)
                     /gene="ssuE"
                     /locus_tag="LAF92_001167"
     CDS             complement(25884..26459)
                     /gene="ssuE"
                     /locus_tag="LAF92_001167"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBI9790212.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            26871..27356
                     /locus_tag="LAF92_001168"
     CDS             26871..27356
                     /locus_tag="LAF92_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9790213.1"
                     /translation="MAADDNAITDGKVTFNGKVIAPACTLVAATKDSVVTLPNVSATK
                     LQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVA
                     LQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIATGKATSGNVNAVTNFHINY
                     Y"
     gene            27439..28140
                     /locus_tag="LAF92_001169"
     CDS             27439..28140
                     /locus_tag="LAF92_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI9790214.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            28165..30765
                     /locus_tag="LAF92_001170"
     CDS             28165..30765
                     /locus_tag="LAF92_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBI9790215.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFPSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            30756..31826
                     /locus_tag="LAF92_001171"
     CDS             30756..31826
                     /locus_tag="LAF92_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9790216.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            31838..32380
                     /locus_tag="LAF92_001172"
     CDS             31838..32380
                     /locus_tag="LAF92_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9790217.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHICDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            32388..32903
                     /locus_tag="LAF92_001173"
     CDS             32388..32903
                     /locus_tag="LAF92_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI9790218.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            32869..33606
                     /locus_tag="LAF92_001174"
     CDS             32869..33606
                     /locus_tag="LAF92_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBI9790219.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            33717..34727
                     /gene="pyrD"
                     /locus_tag="LAF92_001175"
     CDS             33717..34727
                     /gene="pyrD"
                     /locus_tag="LAF92_001175"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBI9790220.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            34901..35443
                     /gene="zapC"
                     /locus_tag="LAF92_001176"
     CDS             34901..35443
                     /gene="zapC"
                     /locus_tag="LAF92_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBI9790221.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(35440..36549)
                     /gene="ycbX"
                     /locus_tag="LAF92_001177"
     CDS             complement(35440..36549)
                     /gene="ycbX"
                     /locus_tag="LAF92_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBI9790222.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            36793..38901
                     /gene="rlmKL"
                     /locus_tag="LAF92_001178"
     CDS             36793..38901
                     /gene="rlmKL"
                     /locus_tag="LAF92_001178"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBI9790223.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            38912..40819
                     /locus_tag="LAF92_001179"
     CDS             38912..40819
                     /locus_tag="LAF92_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI9790224.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            40949..42202
                     /gene="pqiA"
                     /locus_tag="LAF92_001180"
     CDS             40949..42202
                     /gene="pqiA"
                     /locus_tag="LAF92_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBI9790225.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            42207..43847
                     /gene="pqiB"
                     /locus_tag="LAF92_001181"
     CDS             42207..43847
                     /gene="pqiB"
                     /locus_tag="LAF92_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBI9790226.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            43844..44407
                     /gene="pqiC"
                     /locus_tag="LAF92_001182"
     CDS             43844..44407
                     /gene="pqiC"
                     /locus_tag="LAF92_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBI9790227.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            44663..44830
                     /gene="rmf"
                     /locus_tag="LAF92_001183"
     CDS             44663..44830
                     /gene="rmf"
                     /locus_tag="LAF92_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBI9790228.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(44900..45418)
                     /gene="fabA"
                     /locus_tag="LAF92_001184"
     CDS             complement(44900..45418)
                     /gene="fabA"
                     /locus_tag="LAF92_001184"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBI9790229.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(45487..47247)
                     /locus_tag="LAF92_001185"
     CDS             complement(45487..47247)
                     /locus_tag="LAF92_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBI9790230.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            47433..47885
                     /gene="matP"
                     /locus_tag="LAF92_001186"
     CDS             47433..47885
                     /gene="matP"
                     /locus_tag="LAF92_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBI9790231.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKHDLQALLGKE"
     gene            complement(47960..49012)
                     /gene="ompA"
                     /locus_tag="LAF92_001187"
     CDS             complement(47960..49012)
                     /gene="ompA"
                     /locus_tag="LAF92_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBI9790232.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(49369..49878)
                     /gene="sulA"
                     /locus_tag="LAF92_001188"
     CDS             complement(49369..49878)
                     /gene="sulA"
                     /locus_tag="LAF92_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBI9790233.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            50097..50726
                     /gene="sxy"
                     /locus_tag="LAF92_001189"
     CDS             50097..50726
                     /gene="sxy"
                     /locus_tag="LAF92_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBI9790234.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
BASE COUNT        12747 a        12435 c        13351 g        12220 t
ORIGIN      
        1 tatggcgact ctggcggtgc caatccacaa tgcggaagaa gcgtttaacc cgaatgcggt
       61 gaaagtggtt ctcgacgctg aagggtatgc actgtacttc tctcgcgcca ccattccttg
      121 ggatcgtgat cgttttgcag aaggccttga aaccgttggc gataacttcc tgcgtcatct
      181 tggtatttat ggctaccgtg caggctttat ccgtcgttac gtcaactggc agccaagtcc
      241 gttagaacac atcgaaatgt tagagcagct tcgtgttctg tggtacggcg aaaaaatcca
      301 tgttgctgtt gctcaggaag ttcctggcac aggtgtggat acccctgaag atcttgagcg
      361 cgttcgcgct gaaatgcgct aatttcactt cacgacactt cagccaattt tgggaggagt
      421 gtcgtaccgt tacgattttc ctcaattttt cttttcaaca attgatctca ttcaggtgac
      481 atcttttata ttggcgctca ttatgaaagc agtagctttt atgagggtaa tctgaatgga
      541 acagctgcgt gccgaattaa gccatttact gggcgaaaaa ctcagtcgta ttgagtgcgt
      601 caatgaaaaa gcggatacgg cgttgtgggc tttgtatgac agccagggaa acccaatgcc
      661 gttaatggca agaagcttta gtacgcccgg aaaagcccga caactggcat ggaaaaccac
      721 catgctggca agaagtggga ctgtccgtat gccgactatt tatggtgtga tgacgcatga
      781 agaacacccc ggccccgatg tcctgctact ggagcggatg cgtggtgttt cggtggaggc
      841 accagcccga acaccagaac gctgggaaca actcaaagac caaatcgttg aagccttact
      901 ggcctggcac cgtcaggaca gtcgcggttg cgtcggcgcg gtcgacaata ctcaggaaaa
      961 tttctggccc tcatggtacc ggcaacatgt tgaagtgcta tggaccacgc tcaatcagtt
     1021 caataacacc ggtctgacga tgcaggataa gcggatcctg tttcgcactc gcgaatgtct
     1081 cccggcatta tttgaaggct ttaacgacaa ttgtgtgctg attcacggta acttctgttt
     1141 acgcagcatg ttgaaagatt cgcgcagcga tcagttactg gcgatggtcg ggccgggact
     1201 aatgctttgg gcaccgcgag aatacgaact gttccgacta atggataatt ctctggcgga
     1261 agatttgctc tggagttacc tgcaacgcgc gccagtggcg gagtcgttca tctggcggcg
     1321 ttggttgtat gtgttatggg atgaagttgc gcaactggtt aataccggac gatttagtcg
     1381 gcgcaacttc gatctggcat caaaatcact cttgccgtgg ctcgcctgac gaacctttta
     1441 gccactgcca gatacggcca agcgtttcat agccaacacg atcgctatgc atcaaccaga
     1501 ccggagaagg gattgcccgt tcccacgggt taagcggcga gtcgatggcc aactgattcg
     1561 ccggggctgg tagcggattt aacccttcct gctgaaaaaa gatcatcgcc cgcggcagat
     1621 gtgatgcgga ggtcaccagc aggaaagggg cgtcaccaat cgcctgtttc actgccgcag
     1681 cttcttcttc ggtatctttt ggcaaatcca gggtgataat ttgctcgtgc ggcacgccca
     1741 gcgattgcgc aactctggca cctacttccg ctgtactcac cgtattggtt tttgctacgc
     1801 ctcccgtgaa gatcagtttt gatcccggat tttcgcgcca taagcgaata ccttcattca
     1861 ggcgtggcag actgttattg attaaattag agctcggtgc ccactgtggg ttccaggtat
     1921 aaccacctcc gagcaccacg atatagtcca ctttttgcga attattccat gtcggatagg
     1981 tgctttcgat gggacgcagc aggcggtcgg ctaccggttg caaactcaat aacaagagtg
     2041 ccagccaccc aatgctaata aaaatcttcc cggttttttg aaaacggcta aaccacagca
     2101 gcgccaggcc agcgccaata attaacaaca tcagcggaag tggcagcaac atattgccaa
     2161 tcactttttt cagtgtaaaa agcatccttt ctggttcctt ttttaaccat atagcaaggg
     2221 atcgccagcg atattacacc agacgggttc attctcgccg tggctgtgac aaaatagcgt
     2281 ctttatcaga aaacgtctta gccaaccggt ggagaaagca atgcaggatc gcaattttga
     2341 tgatattgcg gaaaagtttt cccgtaacat ttacggcacc accaaagggc agcttcgaca
     2401 ggctattctg tggcaggatc tcgatcacgt gctggcggaa atgggcccgc aaaaactgcg
     2461 tgtgctggat gctggcggtg gtgaagggca gaccgcaatc aaaatggccg agcgtgggca
     2521 tcaggtcatt ttatgcgatc tttctgcgca gatgatcgac cgcgcaaaac aggcggcaga
     2581 agcaaaaggt gtgagcgaca acatgcaatt tatacattgc gccgctcagg atgttgcttc
     2641 gcatttggaa acgcccgttg atctgatatt gttccatgcg gtgctcgagt gggtggccga
     2701 tccccgcagt gtattgcaga ccctgtggtc agtgttgcgt ccaggcggcg tgttgtcgtt
     2761 aatgttctac aatgcgcatg gtttgttgat gcataacatg gtcgccggga attttgatta
     2821 cgtgcaggcg ggaatgccga aaaagaaaaa acggacgctt tcgccagatt atccacgcga
     2881 cccggcgcag gtttatctgt ggctggaaga agctggttgg caaattatgg gtaagacagg
     2941 cgttcgcgtg tttcatgatt atctgcgcga gaagcaccag cagcgcgact gctatgaagc
     3001 attacttgaa ttagaaacgc gttattgccg tcaggaaccg tatattaccc tggggcgtta
     3061 tattcatgtc accgcgcgca aaccgcagag caaggataaa gtatgagtga attttcccag
     3121 acagtccccg aactggttgc ctgggccaga aaaaatgact tctccatctc gctgccggta
     3181 gaccgactct cttttctgct ggcggttgcc acgctgaacg gcgagcgtct ggatggtgag
     3241 atgagtgaag gcgagctggt ggatgcattc cgccatgtga gtgatgcgtt tgagcaaacc
     3301 agcgaaacca tcggcgtgcg cgccaataac gcgatcaacg acatggtgcg tcaacgtctg
     3361 ctgaaccgct ttaccagcga gcaggcggaa gggaacgcaa tttaccgtct gacgccgctc
     3421 ggcatcggca ttactgacta ctacatccgt cagcgcgagt tttctacgct gcgtctttct
     3481 atgcagttgt cgattgtggc gggtgagctc aaacgcgcag cagatgccgc cgaagagggc
     3541 ggtgatgaat ttcactggca ccgtaatgtc tatgcgccac tgaaatattc ggtagcagaa
     3601 attttcgaca gtatcgacct gacgcaacgt ctgatggacg aacagcagca gcaggtgaag
     3661 gacgatatcg cccagttgct gaacaaagac tggcgggcgg cgatttccag ctgtgaattg
     3721 ttgctttcgg aaacttccgg gacgctgcgt gaattgcagg atacgctgga agcggcaggc
     3781 gacaaattgc aggctaatct gttgcgcatt caggatgcga cgatgaccca tgacgatctg
     3841 catttcgtcg atcgtctggt gttcgatctg cagagcaaac tcgatcgtat tatcagttgg
     3901 ggccagcaat ccatcgactt gtggattggc tacgaccgcc acgtacacaa atttattcgt
     3961 accgcgatcg atatggataa aaaccgcgtc tttgctcagc ggttacgtca gtcggtacaa
     4021 acctattttg atgagccgtg ggcgctaact tatgccaatg ccgatcgtct gctggatatg
     4081 cgtgacgaag agatggcact gcgcgatgaa gaagtgactg gggaacttcc tgaggatctg
     4141 gaatacgaag agtttaacga gatccgcgaa cagctggcgg cgatcatcga agaacaactt
     4201 gccgtgtaca aaaccagaca agtgccgctg gatcttggtc tggtggtacg cgaatatctg
     4261 tcacagtatc cgcgtgcacg tcactttgac gttgcgcgta ttgttattga tcaggcggta
     4321 cgtcttggcg tagcgcaagc agatttcacc ggactgccag cgaaatggca gccgattaat
     4381 gattacggag ccaaggtaca ggcgcatgtc atcgacaaat attgaacaag tgatgccggt
     4441 taagctggcg caggcgctgg cgaatccgtt atttccggcg ctggacagcg ccttacgttc
     4501 aggccgccat attggcctcg acgaactgga taatcatgca ttcctgatgg attttcagga
     4561 atatctggaa gagttttacg cgcgttataa cgttgagctt attcgcgcac cagaagggtt
     4621 cttctattta cgcccacgtt ccaccacgct gatccctcgt tccgtcttgt cggaactgga
     4681 tatgatggtc gggaaaatcc tctgttatct ctatctcagc ccggaacggc tggcgaatga
     4741 ggggattttc acccagcagg aactgtacga cgaactgctc accctggccg atgaagcaaa
     4801 actgctgaaa ctggtgaaca accgttcaac cggttcagac gttgaccgtc agaagttgca
     4861 ggagaaagta cgttcttcgc tcaaccgtct gcgtcgttta ggcatggtgt ggtttatggg
     4921 ccacgacagc agcaagtttc gcattaccga atcggtgttc cgcttcggag ccgatgtgcg
     4981 tgctggcgac gatccccgtg aagcacagcg tcgcctgatt cgtgatggcg aagcaatgcc
     5041 gattgaaaat catctgcaac tcaacgatga aaccgaagaa aatcagccag atagcggaga
     5101 ggaagaataa tgattgaacg cggtaaattt cgctcactga cgctgattaa ctggaacggc
     5161 ttttttgccc gaacttttga ccttgacgag ctggtcacga cgctttctgg cggtaacggg
     5221 gcgggtaaat ccaccaccat ggcggcgttc gttacggcgc tgatccccga cctgaccctg
     5281 ctgcatttcc gtaacactac ggaagccggg gccaccagcg gttcgcgcga taaaggtctg
     5341 cacggtaagc tgaaagcggg tgtctgttat tcgatgctcg acaccattaa ctcgcgccac
     5401 cagcgcgtgg tggtcggtgt gcgtctgcaa caggttgccg gacgcgatcg taaagtggat
     5461 atcaaaccat ttgccattca gggactgccg atgtcggtgc agccgacaca gctggtgacc
     5521 gaaaccctga acgaacgcca ggcgcgcgtg ctgccgctta acgagctgaa agacaagctc
     5581 gaggcgatgg aaggcgtgca gtttaaacag ttcaactcca ttaccgatta ccactcgctg
     5641 atgttcgatc tgggcatcat cgcgcgtcgt ctgcgctccg catctgaccg tagcaaattc
     5701 tatcgtctga tcgaagcttc gctgtatggc gggatctcca gtgccattac ccgttctctg
     5761 cgcgactacc tgttgccgga aaacagcggc gtgcgtaaag cgttccagga catggaagcg
     5821 gcgctgcgtg aaaaccgcat gacgctggaa gcgattcgtg tcacccagtc ggaccgtgac
     5881 ctgtttaagc atctgatcag cgaagctacc aactacgtgg cggcggacta catgcgtcac
     5941 gccaacgagc gccgtgtcca tctcgacaaa gccctggagt ttcgtcgcga gttacatact
     6001 tcgcgtcagc aactggcggc tgagcagtac aaacacgtcg atatggcgcg tgagctggca
     6061 gagcacaacg gtgccgaagg cgatctggaa gcggattatc aggcggccag cgatcacctg
     6121 aacctggtgc aaaccgcact gcgtcagcag gagaagatcg aacgctacga agcggatctc
     6181 gatgagctgc agatccgtct ggaagagcaa aacgaagtgg tggcagaagc catcgaacgc
     6241 caggaagaga acgaggctcg cgcggaagct gccgaactgg aagtggacga gctgaaaagc
     6301 cagcttgctg actaccagca ggcgctggac gtccagcaaa cgcgcgcgat ccagtataac
     6361 caggcgattg ctgcgcttaa tcgcgccaaa gaactgtgcc atctgccgga cttaaccgcc
     6421 gacagcgccg ccgaatggct ggaaaccttc caggcgaaag agctggaagc gactgaaaaa
     6481 atgctctctc ttgagcagaa aatgagcatg gcgcaaaccg cgcacagcca gtttgagcag
     6541 gcttatcagc tggtggtggc aatcaacggc ccgctggcgc gtaacgaggc gtgggatgtc
     6601 gctcgcgaac tattgcgcga aggggtcgat cagcgtcacc tggcagagca ggttcagccg
     6661 ttgcggatgc gcttaagcga actggaacag cgtctgcgcg aacagcaaga agctgagcgt
     6721 ctgctggcag atttctgcaa acgtcagggc aagaattttg acattgacga gctggaagct
     6781 ctgcatcagg agctggaagc gcgcattgcc tctctttccg atagcgtgtc taacgcccgt
     6841 gaagagcgca tggcactgcg ccaggaacag gaacagctgc agtctcgcat tcagagtttg
     6901 atgcagcgtg cgccggtttg gctggcagcg caaaacagtc tcaaccagtt gagcgaacag
     6961 tgcggcgaag agtttacctc cagccaggac gtcaccgaat atctgcaaca gttgctggag
     7021 cgtgagcgag aggcgattgt tgaacgcgat gaagtgggcg cgcgcaaaaa cgccgtcgat
     7081 gaagagatcg aacgtttaag ccagcctggc ggctctgaag atcagcgtct gaacgcgctg
     7141 gcggagcgtt ttggtggtgt gctgctgtca gaaatttatg acgacgttag cctggaagat
     7201 gcgccgtact tctcagcgct gtatggcccg tcacgccacg ccatcgtggt gccagatctg
     7261 tcacaggtaa ctgaacacct ggaaggcttg accgattgcc cggaagatct ctatctgatc
     7321 gaaggagatc cgcagtcatt cgatgacagc gtgttcagcg ttgatgagct ggaaaaagcg
     7381 gtagtggtga aaatcgccga tcgtcagtgg cgttattcac gtttcccgga agtgccgctg
     7441 tttggtcgtg ctgcgcgtga aagccgtatt gaaagcctcc atgccgagcg tgaagtgctt
     7501 tccgaacgct tcgccacgct ctcctttgat gtacagaaaa ctcagcgtct gcatcaggcg
     7561 ttcagccgct ttatcggcag tcatctggcg gttgcgtttg agtctgaccc ggaagcagaa
     7621 atccgtcaac tgaacagccg tcgcgtcgaa ctggagcggg cgttgagtaa tcatgaaaat
     7681 gataaccagc agcagcgtat tcagtttgag caggcgaaag aaggcgttac ggcgctgaac
     7741 cgcattctgc cgcgtctcaa cctgttggct gatgacagcc tggcggatcg cgtcgatgaa
     7801 atccgcgaac gtctggatga agcccaggaa gccgcgcgtt ttgttcagca gtttggcaat
     7861 caactggcga aactggaacc gatcgtttcg gtattgcaga gcgacccgga acagttcgaa
     7921 cagctaaaag aagattacgc gtactctcag cagatgcagc gcgatgcccg tcagcaggcg
     7981 tttgccctga cggaagtggt gcagcgtcgt gcgcacttta gctattctga ctcggcagaa
     8041 atgctgagcg gtaatagcga tctcaacgaa aagctgcgtg aacgtctgga acaggcggaa
     8101 gcggagcgta ctcgcgctcg tgaagcgttg cgtggtcacg cagcgcagtt gagtcagtac
     8161 aaccaggtgc tggcttcgct gaaaagttct tacgacacca aaaaagagct actcaacgat
     8221 ctgcaacgtg aattgcagga tatcggcgtg cgtgctgata gcggggcaga agagcgggcg
     8281 cgtattcgcc gtgacgagct gcatgcgcaa ctgagcaata accgttcacg ccgcaatcaa
     8341 ctggaaaaag cgcttacctt ctgcgaagcg gagatggaca acctgacccg caaactgcgc
     8401 aagctggagc gggattactt tgagatgcgc gagcaggtag tgaccgccaa agcgggctgg
     8461 tgtgcggtga tgcgcatggt gaaagataac ggcgttgagc gccgcttaca ccgtcgtgag
     8521 ctggcttatc tctccgctga tgatttgcgc tctatgtcgg ataaggcatt gggtgcgctg
     8581 cgtctggcgg tggcggataa cgaacatctg cgcgacgtgc tgcgcatgtc ggaagatccg
     8641 aaacgtccgg agcgtaaaat tcagttcttc gtggcggttt atcagcatct gcgtgaacgt
     8701 attcgtcagg atattattcg taccgatgat ccggtggaag ctatcgaaca gatggagatc
     8761 gaacttagcc gtctgaccga agaattaacc tcccgtgaac agaaactggc gatcagttcc
     8821 cgcagcgtgg cgaacatcat tcgcaaaacc attcagcgcg agcagaaccg tatccgtatg
     8881 ctcaaccagg ggttgcagaa cgtatcgttt ggtcaggtga acagcgtgcg cctcaacgtg
     8941 aacgtgcgtg aaacgcacgc catgctgctg gatgtgctct ctgaacagca cgagcagcat
     9001 caggatctgt ttaacagcaa ccgtctgacc ttctcggaag cgttggcgaa attgtatcag
     9061 cgtcttaatc cgcagattga tatgggacag cgcacgccgc agaccattgg cgaagaactg
     9121 ctggattacc gcaactatct ggaaatggaa gttgaggtta accgtggttc cgatggttgg
     9181 ttgcgcgcag agtctggtgc attgtcgacc ggtgaggcga ttggtaccgg tatgtcgatt
     9241 ctggtgatgg tggtacaaag ctgggaagat gaatctcgcc gcctgcgcgg taaagatatc
     9301 tctccttgcc gcctgctgtt cctcgatgaa gcagcgcgac tggatgctcg ttctatcgcc
     9361 acgctgtttg aattgtgtga gcgtttgcaa atgcagctca tcatcgcagc gccggaaaat
     9421 atcagcccgg agaaaggcac cacctataaa ctggtgcgta aagtcttcca gaataccgaa
     9481 cacgttcacg tcgtcggcct gcgaggattt gcgccgcaac tccctgaaac gcttccagga
     9541 agtgacgaag cgccttctca ggcgagttaa aattaaggcg gcagcaatgc cgccttttct
     9601 ttttccgaaa actccgtttc cgcactaaaa aagtggcaca ttacggcgcg cttttcttta
     9661 aacttcttta cattaggtta tgtaaaaacg tatcggcgtt tatatactga agataagcct
     9721 gatgagtaac aggcttgctc gtcatacttt cgtgagtatt ggcgttgtac aggcaagtcg
     9781 taaaataaca gcctggctat tcagagtatg ataaaaacag ggggcaaggg atgttgctta
     9841 atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg cctggccgta acattcgctc
     9901 cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc tggcgacagc ccggtggctg
     9961 tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac ggcaataatg gcggggatcc
    10021 agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca aatcgaatct caattacccg
    10081 caggttacaa gccggtttat cttaaccagc ttcaactgtt gtatgccgca cgcgatatgc
    10141 aacccatgtg ggaaaaccgt gatgctgtta aagccttcca gcaacagctg gcagaggtgg
    10201 cgattgccgg tttccagccg cagtttaata aatgggtaga gttactgacc gatcctggtg
    10261 ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat gatgggctat ctccatttca
    10321 ttgcaaatat tccggtcaaa ggcactcgct ggctatatag cagtaaacct tatgcacttg
    10381 caacgccgcc gctctcggtg attaaccaat ggcagctggc gctggataaa ggtcaattgc
    10441 ctacgtttgt tgctggactg gcaccgcagc atccgcaata tgcggcgatg catgaatcgt
    10501 tactggcctt actcagtgac accaaaccgt ggccccaact gaccggcaaa gcaacgttgc
    10561 gcccagggca gtggagtaac gacgtaccgg cgttgcgcga aatattgcaa cgcacaggca
    10621 tgttggacgg ggggccgaaa attactctac ctggcgatga cacgccaact gacgcggtag
    10681 tcagcccatc cgctgttact gttgaaacag cagaaactaa gccgatggac aagcaaacga
    10741 catctcgtag taaacctgcg cctgccgttc gcgccgccta cgataatgaa ctggtggaag
    10801 ccgttaaacg ttttcaggca tggcaaggat tgggggcaga tggtgctatt ggcccggcaa
    10861 cacgtgactg gttaaacgta acgcccgccc agcgtgctgg cgtgttggct ctcaacatcc
    10921 agcgattgcg cttgctgcca acagagcttt ctaccgggat catggttaac attccggcct
    10981 attcgctggt ctactatcag aacggcaatc aggtgctgga ttcgcgagtc attgtcggtc
    11041 gccccgatcg caaaacgccg atgatgagca gtgcccttaa caacgtagtg gtaaacccgc
    11101 cgtggaacgt acctccaact ctggcacgca aagatattct gccaaaagtg cgcaacgatc
    11161 cgggatatct cgaaagccat ggctatacgg tgatgcgcgg ctggaacagc agagaagcga
    11221 ttgacccatg gcaggttgac tggtctacaa tcacggcctc gaatttaccg ttccgcttcc
    11281 agcaggctcc aggcccacgg aactcgctgg ggcgctataa attcaatatg ccgagttcag
    11341 aggccattta tttgcatgac acgccgaacc acaatctgtt caagcgtgat acacgcgcat
    11401 tgagctcagg ctgtgtacga gtgaataaag cttccgatct ggcgaatatg ctgttgcagg
    11461 atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa gcagggtgat acacgttacg
    11521 tcaatattcg gcagtcgatt ccggtgaatc tctactacct gacggccttt gttggtgcag
    11581 atggtcgtac ccagtatcgt acagatattt acaattatga tctgcctgcg cgatccagct
    11641 cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat gaagtagttc ggggaaatca
    11701 gttgtcgtaa taattagcat gattgggggc gattctctgc agcccccgtc actgctgggg
    11761 ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg cctcttgtgc gccagaagtg
    11821 catataaacg ataacattga cctgtagact tgattatcat ggacaaattc gacgctaatc
    11881 gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc cgccatcctg ccgacccctg
    11941 cgtttgcaac actctctacc ccacgcccgc gcattttgac actcaataat cttcataccg
    12001 gagagtcaat caaagcggag tttttcgatg gcagaggcta tattcaggaa gaattggcaa
    12061 aacttaacca ttttttccgc gattaccgcg cgaacaaaat aaagtccatc gacccaggat
    12121 tattcgacca gttgtatcgc ctgcaagggt tgttaggcac gcgcaaaccg gtgcaactca
    12181 tttccggtta tcgttctatt gataccaaca atgaactacg cgcccgcagc cgtggagtag
    12241 cgaagaaaag ctatcacact aaaggccagg cgatggattt ccatattgaa ggtatcgcgt
    12301 taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg tggtgtagga tattatccac
    12361 gtagtaactt tgtgcatatt gataccgggc cagcacggca ctggtagtaa ttgcttaacg
    12421 aaacaggggc agtatgaact ctcgtattat tccggtcacc gcattctccc agaactgttc
    12481 attaatctgg tgtgaacaaa cccgtctggc cgcactggtc gatcctggcg gcgatgcgga
    12541 aaaaatcaaa caggaagttg atgacagcgg cctgacactg atgcagatcc tgctgacgca
    12601 tggtcatctg gaccacgttg gcgcagcggc ggaactggcg caacattacg gcgtgccggt
    12661 ttttggcccg gaaaaagaag atgagttctg gctgcaaggc ttgcctgcgc aaagtcgtat
    12721 gtttggtctg gaagagtgcc agccgctgac gccagatcgt tggctgaacg aaggcgatac
    12781 catcagcata gggaatgtga ctttacaggt gttacattgc cctgggcata cgccgggtca
    12841 tgtcgtgttt tttgatgatc gggcaaagct gctgatttct ggcgatgtta ttttcaaagg
    12901 cggagtaggg cgcagtgact tcccgcgtgg cgatcataat caactgattt cttcaatcaa
    12961 agataaattg ctgccactgg gggatgacgt gacatttatt ccgggtcacg gaccattatc
    13021 cacacttggt tatgaacgcc tgcataatcc cttcctgcaa gacgaaatgc ccgtctggta
    13081 aggcacataa aaaagcccgc ttttaatgcg ggcctggatt tctggcaaag tgcgctttgt
    13141 ttatgccgga tgcggcacga gcgccttatc cggcctacaa aatcgtgcaa attcaaaata
    13201 ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag gcaatgttgc gtttgtcatc
    13261 agtctcagcc cgcttttcag cgggcttcat tgtttttaat gcttacagca ctgccacaat
    13321 cgcttcgcac agcggagcca tgttatctgg tgtcatcccg gccacattta cgcgaccaga
    13381 agcaaccgca tatacgccaa actcttcgcg cagacgcagc acttgttctt ttgtcaggcc
    13441 actgaaggag aacatgccgt tctgtttgat gataaagctg aagtcgcggt ttgcgccttt
    13501 ttcctgcagc gtattgacga acaactgacg catacgctga atacgctggc gcatatcagt
    13561 cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc aggatggtgg caacaacaga
    13621 agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc gccgctttca tttggctgaa
    13681 tgcgcgatca acggtttcac tgtcggcagc aaccagagta caagcgccaa cacgctcgtt
    13741 gtacaggcca aagtttttag agtaggaact ggcaacaatc agctctttat gcatagccgc
    13801 gaaagcgcgc agtccttcag catcttcttc cagaccacgg gcaaaaccct ggtaagcgaa
    13861 gtcaaacagc ggtaaccagc ctttctcaac tgagagttgt gccagtgtct gccattgttc
    13921 cagcgtaggg tcgataccgg ttgggttatg gcagcagcca tggaacagca ctacgtcgcc
    13981 agcctgagct tcgttcaggc tgttaatcag tgcatcgaag tcaagggtgt ggttttctgc
    14041 atcataataa gcgtattcac gaacttccag acctgcagag ttaaagacgc tcttatggtt
    14101 cggccagctt gggttgctca cccacactcg cttaacgctg gtattttttg ccaggaaatc
    14161 ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt gccgtgcgag cacgtttgtc
    14221 attgatcagg gcgctacctt taccaaacag cagttcctga gtgcagcgac caaattcagg
    14281 gatgccgtca atgccgaggt aatttttggt ggtttcattt tcgagcagat actgttcagc
    14341 ctttttcacg ctggtcaata ccggggtttt gcccgtctca tctttataga caccaatccc
    14401 gaggttaatt ttgccgggac gttcatcggc acgaaacaga tcggccaggc ccagaatcgg
    14461 gtcggcagga gcggcggtaa tgttctcaaa catgacgagg ttccattatg gttacagaag
    14521 ggaagtccgc tatcagggta acgggagatt tacaaaattc caactattac tgatgaaaac
    14581 gcaggctgtt tttgcaagac gtgagattgc tctggaaggc ataaaaaaaa caggaccaaa
    14641 gtcctgtttt ttcggcattt aacaaagagg tgtgctatta gaactggtaa acgataccca
    14701 cagcaacggt gtcgtcagaa cctacgccca gtttgttgtc agaatcgatc tggttgatga
    14761 tgtagtcaac ataggtggac atgtttttgt tgaagtagta ggttgcgccc acttcaaagt
    14821 agttcaccag atcaacatca ccgatacctt ctacgtcttt cgctttagat ttggtgtaag
    14881 cgatggacgg acgcagaccg aaatcgaact ggtattgcgc aactaacaga acgtcttgcg
    14941 ttttgttggc gaagccgctg gtgtttgtaa atttattagt gatcggcgta gcgttacggg
    15001 tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc gtacttcaga ccagtagccc
    15061 actgttcagc ttttttaccg ttgccaagag gttgagcttc ttgcaggttg gtacggtcag
    15121 ctgcaccata agcaccaacg ataccaaagc cttcgtattc gtagctgata gaaccgccaa
    15181 caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt tttacccagg tactgaacag
    15241 cgaagttcag gccatcaacc agaccaaaga agttggagtt acgataggta gcaacgccgc
    15301 caacacgacc aacgaagaag tcatcgctgt atgcagtatc accaccaaat tctggcagca
    15361 tatcggtgta acccagtgca tcataaacca caccgtagtt acggccgtaa tcgaaagaac
    15421 caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt tttgttacca gtttgagcgt
    15481 cagcgccttc agagttgtta ccctggaagt tatattccca ctgaccataa ccggtcagat
    15541 cggaattgat ttgagtttcc cctttaaaac caagacgggc ataggtcatg tcgccattgc
    15601 caccgtaact gttttcaccg ttacccttgg aaaaataatg cagaccaaca gctttaccgt
    15661 acagatctac tttgttgcca tctttgttat agatttctgc agcgtttgca gtacctgcta
    15721 ctaacagagc agggacgatc actgccagaa tattgcgctt catcattatt tattaccctc
    15781 atggtttttt ttatgacacc tgccactgcc gtcaataagt tctgtcaata aaaatttacg
    15841 gaactattga tgagagtttg gtgtctttat gtgtctgcag gcatctttcc attcaaacta
    15901 acgtttcgct accgtgaaag tgctacaaag ataaagattt agtttcaaaa agaaaaaata
    15961 tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg tcatatttcc atagaaacgt
    16021 gatgatattt acagcaattt ttatctattt atatgattta cttatattta aattaactaa
    16081 acggaaattt tgtttctgat ggaaacttta tcgacctggc acaaaatcct ctttcagaca
    16141 tccagaatgc caaaaaatag tatgaaaatt gtgctatcag ctaagaaaaa actaatccgc
    16201 aataagaatg cgggttattt tttgtgcgta aatgttgcaa tgtaatattc cgtaacagga
    16261 tgatcgttat ggcggggttg caaatagatt gcttgactta acaaacagat aacttgacag
    16321 aaaggataaa aaagagccag caggcgctgg ctctttgagg aagaaaatca gaagctggcg
    16381 ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt tttgcacgcc agttacgtaa
    16441 gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg gaacagtacc gtagcgacgc
    16501 agatcgcgat accaccagta atcttcttta ttcaggccca tttccagcat acgctcgtcc
    16561 agaacgtcca gacgttcttc acgctgggag ccaccaatga tctcaccgat gcccggagcc
    16621 agaacgtcca tagccgcaac ggttttaccg tcttcgttga ggcgcatata gaacgcttta
    16681 atatctttcg gatagttttt aaccactacc ggtgctttaa agtgttcttc cgccagataa
    16741 cgctcatgct cagaagagag atcgacgccc cagtaaaccg ggttttcaaa cttcctgccg
    16801 cagttttcga gaatggtcac tgcgtcggtg taatccacct gcgcgaaatc ggcctcaatg
    16861 aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt cagcgaagaa tttcatgtcg
    16921 tctgcgcgtt cttcgagaac cgctttgaag acatatttca gcatggcttc agccagaccc
    16981 gcgatatcgt tcaggttagc aaacgccact tccggctcca gcatccagaa ttccgccagg
    17041 tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc cgaaggtata aattttggat
    17101 aatgcgcaag cgtaggtttc gccgttcaac tggccagata cggtcaggaa agactcttta
    17161 ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt tacgcggcag gttttccaga
    17221 tccagcgtag aaacgcggaa catttcgcct gcaccttcgg tatcagatgc ggtaatcagt
    17281 ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc gatgcagcgc ctgcgccagc
    17341 gtatgacgaa cgcgcgcgac ggcaccaatc aggtttgtgc gcggacgcag gtgagcgact
    17401 tcacgcagat actcaatgct gtggcgtttt gccgccatcg ggtaagtgtc tggatcttca
    17461 acccaaccag caacttcaac cttgctggcc tgaatttcaa attgttgccc ctggcccggc
    17521 gacgccacga ctttacccgt cacaatgacc gagcagccgg tggtcagacg caggacgtct
    17581 tcattgtaat tgggcagaga attattgatg acagcctgta caggatcaaa gcaggaaccg
    17641 tcataaacgg cgaggaagga gatgccagct tttgaatctc ggcgggtacg tacccatccg
    17701 cgtacggtga cttcgctgtc aacggctacg cggccctgga gtacgtcggc tacaggcaca
    17761 acgctcataa tattctctct gttaatagtc ggaaaaaata aacacttgtc cacccgtaaa
    17821 tggggggata cctatgttac ctggcatctg caatcagaca agcagaattc gcaaatgcag
    17881 cgaaagattt cggaattaaa ggtaaaaaaa gggagccgat tagctccctg atgatattaa
    17941 ctggcttttt taatatgcgg aaggtcgaac gctttgcgca gcgcccgaac aaacgcttta
    18001 tcgtggcaga tagttttgcc agggctgtca gaaagtttcg ccaccggttt accgttacac
    18061 tcaaccaact taatcacaat attcaggggt tttacctggg ggatatcgca ggtcaggcga
    18121 gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt ggcggtatag ctcaaccgct
    18181 ttgcgtaaat ccagattgtc agagaaaacc agagttttac tctgtggatc aattcccagc
    18241 ttttcataat gtgcaatggc tttttcaccc cattcaaccg ggtcgccaga gtcatgacgc
    18301 aggccctgat agcgactggc gaactcgaca ccgaaatcac gcaggaaagc atccatagtg
    18361 atgcagtcgg ttaatgcaat gccaagttgg tcgggatatt cttccagcca ggcagcaagt
    18421 gcagctcgct ggctgttggc tagatccggg ctgatttgct gatgtgcctg gaaccattcg
    18481 tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg ccagatcgta gttgctggtg
    18541 cccacgaacc aggattcctg ttgcagacgc ttaacgatgg tttcttgtac ttcgcgagaa
    18601 aaacggcgac gggtgccaaa atccataaga tggaagcgcg acatatcaag gccggcggtt
    18661 aacgccgaga agtcgactaa tttgctttcc agcgtgtcga gggcttgcgc aacgtcggcc
    18721 tgcggtgagc gatagcgatg taccatttca ctgataaccg ccagcaaagg aacttcccag
    18781 aggatgactt cacgccacgg gccgcttaaa cgaatatcca gcttgccatt atcgttggat
    18841 acggtgactt gttccgggtt aaagcggaac tcgcgtaacc agttaagata gtcggcctta
    18901 aagaaaggca gagcagagag ccactgatat tcatcatcct gcaggcgcag gtgctgcatc
    18961 gcctgaatct gttcacgaat agcatcggca taaataccca gcagatcgtc acctcggcaa
    19021 cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa acacggcttg ctgcatatgc
    19081 aacttataag catctgtatc cagcaacgag tgcagaacag gagaagcgaa ttgtgtcata
    19141 ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa taaacatctt caggaactgc
    19201 tggagtatac cttgtttcgc aatttattga accccgatca caccatatgc cacctttctg
    19261 gtcgatggca ttacgcggtg tatgttataa aaatgtagca ataaaggcgt ttgtacctga
    19321 aaagatgaag attctgcata gcgcgattta cgcaacagga atagactgaa caccagactc
    19381 tataaaagat gctaaaggtt atttatgact caacagccac aagccaaata ccgtcacgat
    19441 tatcgtgcgc cggattacca gattactgat attgacttga cctttgacct cgacgcgcaa
    19501 aagacggtcg ttaccgcggt cagccaggct gtccgtcatg gtgcatcaga tgctccgctt
    19561 cgtctcaacg gcgaagacct caaactggtt tctgttcata ttaatgatga gccgtggacc
    19621 gcctggaaag aagaagaggg cgcactggtc atcagtaatt tgccggagcg ttttacgctt
    19681 aagatcatta atgaaataag cccggcggcg aatacggcgc tggaagggct ttatcagtca
    19741 ggcgatgcgc tttgcaccca gtgtgaagcc gaaggtttcc gccatattac gtattatctc
    19801 gaccgcccgg acgtgctggc gcgttttacc accaaaatta ttgccgataa aatcaaatat
    19861 cccttcctgc tttccaatgg taaccgcgtt gcgcaaggcg aactcgaaaa cggacgccat
    19921 tgggtacagt ggcaggaccc gttcccgaaa ccgtgctacc tgtttgcgct ggtggcaggc
    19981 gactttgatg tactgcgcga tacctttacc acgcgttctg gtcgcgaagt agcactggag
    20041 ctgtacgtcg atcgcggcaa ccttgatcgc gcgccgtggg cgatgacctc gctgaaaaac
    20101 tccatgaaat gggatgaaga acgctttggc ctggagtatg acctcgacat ctatatgatc
    20161 gtcgcggtgg atttcttcaa tatgggcgca atggagaata aggggctgaa tatctttaac
    20221 tccaaatatg tgttggcccg caccgacacc gccaccgaca aagattatct cgatattgaa
    20281 cgcgttatcg gccatgaata tttccataac tggaccggta accgagtgac ctgccgcgac
    20341 tggttccagc tcagcctgaa agaaggttta accgtcttcc gcgatcagga attcagctct
    20401 gaccttggtt cccgcgcggt aaaccgtatc aataatgtgc gcaccatgcg cggattgcag
    20461 tttgcggaag acgccagccc gatggcgcac ccgatccgcc cggatatggt cattgagatg
    20521 aacaacttct acaccctgac cgtttacgag aagggcgcgg aagtgattcg catgatccac
    20581 accctgctgg gcgaagaaaa cttccagaaa gggatgcaac tctatttcga gcgccatgat
    20641 ggcagcgcgg caacctgcga cgactttgtg caggcgatgg aagatgcgtc gaatgtcgat
    20701 ctctcccatt tccgccgttg gtacagccag tccggcacgc cgattgtgac cgtcaaagac
    20761 gactacaatc cggaaaccga gcagtacacc ctgaccatca gccagcgcac gccagccacg
    20821 ccggatcagg cagaaaaaca gccgctgcat attccatttg ccatcgaatt gtatgacaac
    20881 gaaggcaaag tgatcccgtt gcagaaaggc ggtcatccgg tgaattccgt gctgaacgtc
    20941 acccaggcgg aacagacctt tgtttttgat aatgtctact tccagccggt gcctgcgctg
    21001 ctgtgcgaat tctctgcgcc agtgaaactg gaatataagt ggagcgatca gcaactgacc
    21061 ttcctgatgc gtcatgcgcg taatgatttc tcccgttggg atgcggcgca aagtttgctg
    21121 gcaacctaca tcaagctgaa cgtcgcccgt catcagcaag ggcagccgct gtctctgccg
    21181 gtacatgtgg ctgacgcttt ccgcgcggtg ctgctcgatg agaagattga tccggcgctg
    21241 gcggcagaaa tcctgacgct gccttctgtc aatgaaatgg ctgaactgtt cgatatcatc
    21301 gacccgattg ctattgccga agtacgcgaa gcactcactc gtactctggc gactgaactg
    21361 gcggatgagc tgctggctat ttacaacgcg aattaccaga gcgagtaccg tgttgagcat
    21421 gaagatattg caaaacgcac tctgcgtaat gcctgcctgc gcttccttgc ttttggtgaa
    21481 acgcatctgg ctgacgtgct ggtgagcaag caataccatg aagcaaacaa tatgaccgat
    21541 gcgctggcag cgctttctgc ggcggttgct gcacagctgc cttgccgtga cgcgctgatg
    21601 caggagtacg acgataagtg gcatcaggac ggtctggtga tggataaatg gtttatcctg
    21661 caagccacca gcccggcggc gaatgtgctg gagacggtgc gttgtctgtt gcagcatcgc
    21721 tcatttacca tgagcaaccc gaaccgtatt cgttcgttga ttggtgcgtt tgcgggcagc
    21781 aacccggcag cgttccatgc cgaagatggc agcggttacc agttcctggt ggaaatgctt
    21841 accgacctca acagccgtaa cccgcaggta gcttcacgtc tgattgaacc gctgattcgc
    21901 ctgaaacgtt atgatgccaa acgtcaggag aaaatgcgcg cggcgctgga acagttgaaa
    21961 gggctggaaa atctctctgg cgatctgtac gagaagatca ccaaagcact ggcttgatac
    22021 ttttgagcca aaatgcctga tgcgacgctt gacgcgtctt atcaggccta caactccgtt
    22081 gaaattgctg gttttgtagg ctggataagg cgttcacgcc gcatccggca gccgtgccca
    22141 actgcctgat gcgatgcttg acgtgtctta tcaggcctac tagtccgttg aaaatgctgg
    22201 ttttgtgcgc cacatccggc atattaacca tgacgccgaa tcagttgttc agaatgccca
    22261 cgccgcatta cccgttgcaa tacttccgct tccagctccg ctaatcgcac cgaccctaat
    22321 cgacgcggac gggggatatc cactgtcaaa tccagaccaa tttttccctc ttcaattaac
    22381 aacacccggt cagccattgc taccgcttcg ctcacatcat gcgtcaccaa cagtacggta
    22441 aagccgtgct cctgccaaag tgacacaatc aaatcctgca tctcgagtcg cgttaaggcg
    22501 tccagtgccc cgagcggttc atcaagcaac aataaacccg gtcgatgaat caacgctctt
    22561 gccagcgcca ctcgctgttt ctgcccgcct gaaagtgcgg caggccattc cccggcgcga
    22621 ttctccagtc ctaccgcagc caacgcccga cgcgcggcat cgcgccactg gcctttaagg
    22681 cctaacccaa cgttatcaat caccgatttc cacggtagca gacgcgcatc ctgaaacatc
    22741 attcgcgtgt cttcctgaat ttcagccagc ggtgtggtgc ctgccaacac atcgcctgcg
    22801 gttggcgttt ccagcccggc cagcaggcgc agcagggtac ttttgccacc accgctgcgg
    22861 cccaccaccg ccacaaactg acctgccgga atatgtaaat ccagttggtt caggacgata
    22921 ttttccgcgt aatgtttgct tactgcattg agcaacaatg gtgtgccctg gttcagacga
    22981 gcagtattca taccgtggcc tccttcaaat gataagccgg gttccagcgc aaccagaggc
    23041 gctctaacag ctgcgcgctg acgtcagcaa gtttgccgag cagggcgtaa agaataatag
    23101 cgactaccac cacgtccgtt tgcaaaaact ctcgcgcatt catcgccaga taaccaatgc
    23161 cggaattggc agaaatggtt tcggcaacaa tcagcgtcag ccacatcagg cccaacgcaa
    23221 aacgcacgcc gaccataatt gagggcaggg caccaggcag gatcacatgg ataaacagcg
    23281 gtatgccgga taacccatag ctacgcgcca tctccaccag cccgcgatcg atattacgga
    23341 tgccatgcca ggtgttgata taaatgggaa acagcgtacc gagcgccacc agaaagattt
    23401 ttgcggattc atcaatgcca aaccacaaaa tcaccagcgg gatcagcgcc agatgcggca
    23461 cgttgcgcaa catctgaatt gaggtatcca gcagccgttc tccccagcga gatagcccgc
    23521 taatcagtcc caaaatcaac cccagcgatc cgccaattga aaagccaatc agcgcccgcc
    23581 tggaactgat cgccagatgc tgccacagtt cgccgctggc ggagagcgtc cagaacgccg
    23641 tcaccacccc ttccggtgag ggcaaaatac gcgtcgacag ccagccaact gaggaggcca
    23701 gttgccacac cgccacgatg cccaccggta aaaaccaggg ggcaacgcgc aataaccact
    23761 tcttcactgg cgttgccatt atttcttcct tagctttgcg cgactttacg ggggataaaa
    23821 tcattcgcca ccgcttcgcc ttgcggattc agcggctgcg gctggggaat ttccggaatg
    23881 gcgacatcca gatgtgggaa cagcaactcg ccgacccgat acgcttcttc cagatgcgga
    23941 tagcccgaaa gcacaaagct gtcgatgcca agcgcggcgt attcgttgat tcgcgcagcg
    24001 accgtaggac catcgcccac cagcgccgtt ccggcaccgc cgcgcactaa gccaacgccc
    24061 gcccataaat tggggctgat ctccagattg tcgcgcttgc cgttatgtaa cgccgccatt
    24121 cgctgttgtc ctacggaatc cgtccgggcg aatgcggcct gtgctttggc gatagtttca
    24181 tcatcaagat gcgagattaa ccgctcggcg gcctgccacg cttcgtcgtt agtttcacga
    24241 acaatcacat gcagacgaat accgaaacga attttgcgtc catgcgcggc agctttcgcc
    24301 cgcacttgtt cgatcttctc ttttactagt tctggcggtt cgccccaggt gaggtagaga
    24361 tcaacttgtt ctgccgccag ctcctgggca acgtctgacg atccgccaaa gtaaagcgga
    24421 ggataaggct gttgaatcgg cgggaagagc agttttgctc cgcgcacatg aatatgttta
    24481 ccgttgaaat tgacggtttc ccccagcaat aagcgccgcc agacctgggt aaattccgcc
    24541 gaggcttcgt agcgctcgct atgatcaagg aacacgccgt cgcctgccag ctcttgtgga
    24601 tcgctgcctg tgaccagatt aaacaacgca cgaccatttg agagacggtc aagcgtggcg
    24661 gcctggcgag cggcaacggt aggtgaggtt acgctggggc gcagggcgac aagaaacttc
    24721 agccgctgcg tcaccgggat catcgacgcc gccaccagcc acgcatcttc acacgatcgc
    24781 cccgttggga tcagcacacc ggtatagccc agacgatccg ccgcttgcgc aatttgttgc
    24841 agatagccgt gatcaaccgg gcgtgaacct tcttccgttc caagataatg cccgtcacca
    24901 tgggtcggta aaaaccagaa catattcaga ctcataattg ttttccttcc agttgagtgg
    24961 gctgccagat gcgctggcga atatcgactt ttttcggcac cagacgattt tcataaaaca
    25021 gatctgccgt ttgctgctgt aaggcggcaa cctcggcgtt aaccggtttg atggtggtag
    25081 gagggcgatg atctaagtaa gaggcaatca ccggtgccgg taagcccatc gtttttgcca
    25141 gtaaagcgat gctttgctcg cgctggctgc gggttaacgc atcggcctca ctaaaggttg
    25201 cgagtacgcc ctgaataaaa gcgccgtttt tttctgcata gggacgagct gccagataaa
    25261 acgatccagt ttgattgaga tcggtgccgt ctttcagcac ccgcacgccg ccctgtaata
    25321 atgcagcaga gtagtaggga tcccagatag cccaggcgtc aacgttacct tgctggaacg
    25381 cggcgcgggc atcggcaggt gtcaggtaag tgggttgtat atcggtaaac ttaagtccgg
    25441 cctgacgcag tgcacgcagt aaaaggttgt gtgaactgga acctttctga aaggcaactt
    25501 tgtgaccttt aagatcggct acggttttga tcgggctgtt ttctgccacc agaatcactt
    25561 cggctttggg cttcggtggc tcgacgccca catataccaa atcagccccg gcagcctggg
    25621 caaagattgg cggaatatcc ccggtactgc cgagatcaat actgccaacg tttaacgctt
    25681 ccaacatttg cggacccgcg gggaactcca cccaggagat ttttgattgc ggatagcgtt
    25741 tttccagtaa ctggtggctt tttgccagca ccataccaat actgcctttc tgatagccta
    25801 tacgtaacgc ttcaggcgag gattctgcag caaccgcaaa agtagagacg ctaagcaatc
    25861 ccgccagcgc cagtttaatg atgttacgca tggacattac ctcgcagaga cagaaggtca
    25921 ggaacctgaa catcgcggcg gtgcaatgcc tgccagaaag tttctagcgc ggtatcaaga
    25981 cgggtttgca gatttggcgt gaactggggt ctgtgatggt aatcaatgac ttgtgagtca
    26041 tcggcaaaca cgccgtgcag gatctcctgg gctttcagtg cgcttaaaac tggtttaagg
    26101 gcataatcga ccgccagcag atgggccacg gtaccgcccg tcgccagcgg tagcaccact
    26161 ttgccttgca aagcgcgctc tggcagcagg tcgagcaggg ttttcagcgc gccggaatag
    26221 gcggctttat acacaggtgt agcgactatc agtccatcag cctgttgcag ctgttcggtg
    26281 aaggtcttga gtgccggact atcgaaacga gcataaagca gatcttccgg ggcgaagttt
    26341 tgcagattcc agtgataaac ctctacatcg aggccattta gtttttcccg cgcatattcc
    26401 agcaaggagc tggagcgaga aggaaagcga ggactacccg ccagggtgat gacacgcata
    26461 ctctctcctt ataaccaatt gttctttttt tgttaacatt gataacaatt cggtcagtct
    26521 gtcggagaga caagaaaatg ccaaatataa attttgtgta tctttttctg aaaataaact
    26581 aaagaaaggg ctttatatat cacgcatatt tatttgttgg atagtcatta gatgttgaat
    26641 caatgaatta ctcgttcagt taatatttaa atattcatga aatctataaa ttaaagattt
    26701 gtcacttatt ggatttagta tgcttgattt aaatggcggt caattattgt tattattaag
    26761 tctaatgtca tttaaaggca ttatcatatt tacgaagaga tcaggatgat gacgatgaaa
    26821 aaagtgtatt gacggcgttt ataactgtgg tatgtgcaac gtccagcgtt atggcagctg
    26881 atgataatgc tatcacggat ggcaaagtaa catttaatgg taaagttatt gctccagctt
    26941 gtaccctggt agctgcgacg aaagattcag tagtcacatt gccaaatgtc agcgccacca
    27001 agttgcaaac taatggagca gtctctggcg ttaaaactga tgtaccaatt gccttagaag
    27061 gctgtgatgt tactgtaact aaaaatgcga cgtttacctt tagtggtacg gctgacggcg
    27121 tgcaacctac agcatttgcc aaccaggcta ccaccgatgc agcgacgaat gttgctctac
    27181 aaatgtatct gccagatggt tctacatcgg taactcccgg taccgaaacg tcgaatattc
    27241 agttagcgga ttctgctgag caaacagtga cctttaaagt tgattatatc gctacgggga
    27301 aagcgacttc tggtaatgtg aatgcggtaa caaatttcca tattaactat tattaataga
    27361 actcattaat tgttttatta attagtaccc ctctagtgtt ctggagggga tattcatatt
    27421 ttttaagagt gactatttat gaaaacttgc ataacaaagg gaattgtgac cgtaagttta
    27481 acggcaatat tactcagctg ctcatccgca tgggcggcag gtaaaggcgg gattggactt
    27541 gcagcaacac gtcttgttta ttcagaaggt gaagagcaaa tttcactggg tgtacgtaac
    27601 accagtccgg atgttcctta tcttattcag tcatgggtga tgaccccaga taataaaaaa
    27661 tcagcagatt ttattattac accaccgcta tttgtgctga atccggctaa tgagaatctg
    27721 ttacgcatta tgtacattgg tgcgccgttg gcgaaagaca gagaaaccct tttcttcact
    27781 agcgtacggg cagtcccttc aacaacgaag cgggaagagg gaaataccct gaagattgcc
    27841 acacaaagcg tcatcaaact tttctggcga ccaaaaggtt tagcgtatcc cttaggcgag
    27901 gctccggcga aactgcgttg cacttcgtca gctgacatgg ttacggtcag taacccaaca
    27961 ccttatttca ttaccctgac agacctgaaa ataggtggaa aagtagttaa aaatcaaatg
    28021 atttccccct ttgataaata ccaattttct ctgccaaagg gggccaaaaa tagcagcgta
    28081 acgtatcgaa ccatcaatga ctacggggcg gaaacgccgc aactcaactg taaatcgtaa
    28141 gccgtcttca gttaagagag cgagatgtat agaactcatc gacaacacag cctgttaagc
    28201 tctggtggag tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc agcgttcaat
    28261 gcacaagctg aaacctggtt cgatcctgct tttttcaaag atgatccctc aatggtggcc
    28321 gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg tgtcgatatt
    28381 gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat aacgccagag
    28441 aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggtgt taatactgat
    28501 gcgtttccgg cttttaaaca actggacaaa caagcgtgtg tgccattggc ggagattatt
    28561 ccggatgcca gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc agtaccgcaa
    28621 atcgccatca aaagtaatgc ccgtggttat gtcccccctg aacgttggga tgaagggatc
    28681 aacgcgctat tactgggata ttcgtttagc ggggctaaca gtattcatag cagcgcagac
    28741 agtgattctg gcgacagcta ttttctgaat ttaaacagtg gcgttaattt aggcccatgg
    28801 agattgcgca acaattcaac atggagtcgc agtagtggcc aaaccgcaga atggaagaat
    28861 ctcagcagct atttgcagcg ggcggttatt ccactaaaag gcgaactgac cgtaggtgat
    28921 gattatactg caggcgattt tttcgatagt gtcagctttc gtggtgtgca gctggcgtca
    28981 gatgacaaca tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg tggtatcgcc
    29041 aaaagcaatg cccagataac gattaagcaa aatggttaca ccatttacca aacttatgta
    29101 tcgcctggtg cttttgaaat tagtgatctc tattccacgt cgtcgagcgg tgatttgtta
    29161 gttgaaatca aagaagcgga cggcagcgtc aatagctaca gcgtaccgtt tcccagcgtg
    29221 ccattactcc agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa atacagaacc
    29281 aatagtaatg aacagcagga gagcaaattt gcccaggcca cgttgcagtg gggcggaccg
    29341 tggggaacga catggtatgg tggtggacaa tatgctgaat attaccgtgc cgccatgttt
    29401 ggtctgggat ttaaccttgg cgatttcgga gcaatttcgt tcgatgtgac ccaggcgaag
    29461 agtacgctgg cagaccaaag cgaacataaa ggtcagtcat atcgttttct gtatgccaaa
    29521 acgctcaacc aattgggcac taattttcaa ttgatgggct atcgctattc gacgtcgggt
    29581 ttctacaccc tttccgacac catgtataaa catatggatg gctacgaatt taatgacggt
    29641 gatgatgaag atacgccgat gtggtcgcgt tattacaatt tgttttacac caaacgtggc
    29701 aaactgcagg tcaatatctc ccagcaatta ggcgagtacg gttcgtttta tttaagtggt
    29761 agccagcaaa cttactggca taccgatcaa caggatcggc tattacagtt tggctacaac
    29821 acgcaaatta aagatctctc gctgggggtt tcctggaact acagtaagtc ccgtggtcaa
    29881 cctgatgctg atcaggtgtt tgcactaaat ttttccctgc cgctcaatct gttgctcccc
    29941 agaagtaatg atagctatac caggaaaaaa aattacgcat ggatgacctc taacaccagt
    30001 atcgataacg aagggcacat tacacaaaac ctgggtttaa cggagacact actcgatgac
    30061 ggtaacctga gctacagcgt gcaacaggga tataacagcg aggggaaaac ggctaatggt
    30121 agcgccagca tggactacaa aggggcgttt gcagatgccc gagtgggcta caactacagc
    30181 gataacggca gtcaacaaca actgaactac gctctttcag gcagtttagt tgcccattca
    30241 cagggcatta ccctggggca atcgctgggg gaaactaacg tcctgattgc agcaccaggc
    30301 gcagagaata ctcgtgtggc gaacagcacc gggctgaaaa ctgactggcg cggatatacc
    30361 gttgtcccct atgccacttc ttatcgggaa aatcgaatcg cacttgatgc ggcatcgtta
    30421 aaacgtaacg tggatcttga aaatgcagta gtcaacgtgg ttcccaccaa aggggcgttg
    30481 gttctggcgg agttcaatgc ccatgcggga gcaagggtat taatgaaaac atcaaagcag
    30541 ggtataccgc tgcgttttgg cgcgatagcg acgctggacg gcatacagac taatagcggc
    30601 attattgatg atgatggctc gctctatatg tccggtttgc ccgctcaggg agcgataact
    30661 gtacgctggg gcgaagctcc cgatcaaatt tgtcatatca gttaccagct taccgaacaa
    30721 caaattaact ctgcgattac gcgaatggat gccatatgca gataatcttt ggagaaaaat
    30781 gcgtgttatt actacgacta ttttttgccg ccgtcttaat gctatggtgc gctcaaactg
    30841 ctgcttatag cgggcagtgt cataccactc aggggaatcc gtatattggc gtcaattttg
    30901 gcgttaaaac cctggaggaa gaagaaaata cggctggagt agttaaagac aaattttatc
    30961 agtggaacga atcgaatgat tattatgttt cctgtgattg cgataaagac aatgtcagaa
    31021 gtggccgatg ggcattcgcc gcggattcac cgttagtcta tttaggcgac aactggtaca
    31081 aaattaatga ctatcttgcc gccaaagttt tattgcaggt taaaggcagt tctcctactg
    31141 cggttccttt cgaaaacgtg ggcacagggg cagatacacg atggcatatt tgcgatcccg
    31201 gcggtcaacg tttaggtggc cagggggcta gcggtaatag cggtagcttt tccctgaaaa
    31261 tattgcagcc gttcgttggt tcggtcgtca ttcctcctat ggcgctggcg cgattatttg
    31321 aatgctacaa catacccgca ggtgattcct gcacgactac aggtacatcg gttttagtgt
    31381 attacctgtc tggtacgatc aattcacttg gctcatgttc cgtcaatgcc ggagagacta
    31441 tcgaggtcga tctgggcgac gtatttgcgg ctaactttcg tgttgtaggg cataaacccc
    31501 ttggggccag aacggcagaa ctcgcaattc cagtcaggtg taacacggga aacgcggggt
    31561 tagttaacgt caacctgagt ctgacggcaa ccacagaccc cagctatccc caggcgatta
    31621 agacgtcacg tcctggcgtg ggcgtggtgg tgaccgatag ccagaacaac attatttccc
    31681 ctgctggtgg aacattaccg ctctctattc ctgatgatgc agacagtatc gcgcgaatga
    31741 atgtctatcc agtcagcacg acaggtgtac caccagaaac cgggcgattt gaagccacgg
    31801 caacggtgag aataaatttt gattaacgcg tgaacgtatg aagaaaaaaa cgatatttca
    31861 gtgcgttatc ttgttcttta gcattcttaa catccatgtc gggatggctg ggcctgaaca
    31921 agttagtatg catatttatg ggaatgtggt cgatcagggc tgtgatgtcg ccaccaaaag
    31981 tgcattacaa aatattcata tttgtgattt taatatcagt gattttcagg ccgcgaatac
    32041 cgtaagcact gctgctgatt tgaatatcga tatcaccggt tgtgccgctg gtattactgg
    32101 cgcggacgtc ctttttagcg gcgaggctga cacccttgcg ccgacactgc tcaaactaac
    32161 tgacacaggc ggaagcggtg gtatggcaac ggggattgcc gtgcaaattc ttgatgcgca
    32221 aagtcagcaa gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa aagccgggga
    32281 taacacactc aaatatcaac ttcgttataa gtccacaaag gcgggagcaa cgggcggtaa
    32341 tgcgacggcg gttctctatt ttgatctggt ttaccagtga aggagtgatg ttgaaacgga
    32401 taatctggat tctgttctta ctgggattaa cggggggctg tgagctattt gcccatgatg
    32461 gcacagtcaa cattagcgga tcgtttcgcc gtaatacatg cgtgctggca caggatagca
    32521 agcaaatcaa tgtgcagtta ggcgatgtct cgctgacacg tttttttcat gggaattatg
    32581 gcccggagaa atctttcatc attaatttgc aagattgcgg aacagatgtc agcacggttg
    32641 acgtcacctt ttcaggaacc cccgacggag tgcagagcga aatgctgtcc attgaaagtg
    32701 gtacggatgc agccagcggg ctggcaattg cgattctgga tgatgcgaag atattgattc
    32761 cgcttaacca ggcgagtaaa gactacagcc tacatagcgg taaggtgcca ttaacttttt
    32821 atgcgcaatt gcgacctgtc aatagcgatg tgcagtcggg taaagtgaat gccagcgcga
    32881 catttgtact tcattatgac taatacgtgg aacagattgg cgctcttgat tttcgccgtt
    32941 ttatcgctgc tggtggcggg tgaattgcgg gcaggagtgg tggtcggcgg aacgcgattt
    33001 atctttccgg cagacagaga atcgatatct attttactga ctaataccag tcaggaatcc
    33061 tggcttatta acagtaaaat caaccgccca acgcgttggg ccggggggga agcgtcgaca
    33121 gtgccagcac cattactggc cgctccgcca cttattctcc tgaagcccgg tactacaggc
    33181 acgttgcgct tgctgagaac ggaaagcgac atcttgcctg tggatcgcga aacgctattt
    33241 gagttaagca ttgccagcgt gccatccggc aaagttgaaa atcagagcgt aaaagtggcg
    33301 atgcgctcgg tatttaaact gttctggcga cccgaaggtt tgccgggcga cccgctggaa
    33361 gcttatcaac aattacgctg gacacggaat tcgcagggtg tacaactcac taacccaacg
    33421 ccttactaca ttaacctgat tcagttgagc gtaaatggta aagcgttaag taatgcggga
    33481 gttgtgccgc ctaaaagcca gcgtcagaca agctggtgtc aggcaatcgc accgtgtcat
    33541 gtcgcctggc gggcgataaa tgattacggc gggttgagtg caaagaagga gcaaaatctg
    33601 ccctgaaaca ggttcggaaa acgtttgcgt tttttttgcc gcaggtcaat tcccttttgg
    33661 tccgaactcg cacataatac gcccccggtt tgcacaccgg gaatccagga gagttcatgt
    33721 actacccctt cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct catgagttta
    33781 cttttcagca attacgccgt attacaggaa cgccgtttga agcactggtg cggcagaaag
    33841 tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa aaatccgctt ggtctggcag
    33901 ccggtcttga taaagacggg gagtgcattg acgcgttagg cgcgatggga tttggatcga
    33961 tcgagatcgg taccgtcacg ccacgtccac agccaggtaa tgacaagccg cgtctctttc
    34021 gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt taataatctt ggcgttgata
    34081 acctcgtaga gaacgtaaaa aaggcccatt atgacggcgt cctgggtatt aacatcggca
    34141 aaaataaaga tacgccagtg gagcagggca aagatgacta tctgatttgt atggaaaaaa
    34201 tctatgccta tgcgggatat atcgccatca atatttcatc gccgaatacc ccaggattac
    34261 gcacgctgca atatggtgaa gcgctggatg atctcttaac cgcgattaaa aataagcaaa
    34321 atgatttgca agcgatgcac cataaatatg tgccgatcgc agtgaagatc gcgccggatc
    34381 tttctgaaga agaattgatc caggttgccg atagtttagt tcgccataat attgatggcg
    34441 ttattgcaac caataccaca ctcgatcgtt ctcttgttca gggaatgaaa aattgcgatc
    34501 aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag caccgaaatt attcgccgct
    34561 tgtcactgga attaaacggt cgcttaccga tcatcggtgt tggcggcatc gactcggtta
    34621 tcgctgcgcg tgaaaagatt gctgcgggtg cctcactggt gcaaatttat tctggtttta
    34681 tttttaaagg tccgccgctg attaaagaaa tcgttaccca tatctaatta tttcttcgaa
    34741 ttcctcttta taagataatc agggctttat tttcagccct ggttgtttta tattcatcgc
    34801 tgttgcttat ttagacattt tgtactttta ttgttgaggt tattaagcga agcgacaatg
    34861 gattgtgttg ctgcggtttt atagggtagg ggagaggcag atgcgaatta aaccagacga
    34921 taactggcgt tggtattacg atgaagagca cgatcgaatg atgctcgatt tagccaatgg
    34981 tatgctattt cgctcacgtt ttgcgcgcaa gatgttgact ccagatgcct tttctcccgc
    35041 aggcttttgc gttgacgacg ccgcgctcta tttctctttt gaagaaaagt gccgcgattt
    35101 taatttatct aaagaacaaa aagccgaact ggtgctgaat gcactggtag caattcgcta
    35161 tctcaaaccg caaatgccga aaagctggca ttttgtttcc catggtgaaa tgtgggtgcc
    35221 catgccgggc gatgcggcct gcgtttggtt gagcgatact cacgagcagg tcaatttgct
    35281 ggtcgttgaa tctggcgaaa atgccgcact atgcctgctg gcacaaccct gcgttgttat
    35341 tgcgggtcgc gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca ggctgaaacc
    35401 gcaggttaat gttgacagct tcagcctcga acaggctgtc taacgcgcca acttaagtgc
    35461 agtcttcggt acacagctac agcaaagaat ggtgccatca tcgcccattg ctgatttttt
    35521 cagcggcgtg acttcgcctt ctaaaagctg aacacggcaa cttccacaaa tgcccgcgcg
    35581 gcaagaataa gggatacgaa ttccctgatt ttctaattgc tccagcaaca cctgttggtt
    35641 atttccacga aatgcctgtc cctgccagtc aatatctaca tttgcgtccg gttgttgcgt
    35701 gatgttggcg gtatcatcag cggcagctgc gccgtaaatt tttgccggag ccgttgccag
    35761 aatttccacc tcatcgccaa cgcgaatcac gccgctatta cgggcaatta aattctgacc
    35821 aaaatcgaca tcgccgttat cctgggcagt gcggaaagat tgcaatgttt ttaatggttc
    35881 gcctgccgga tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac agcggctaca
    35941 aggtttaacc acatcaaaca ccacatcacc aatgcgaatc actttccagc gatcttcttc
    36001 ccaggctgac gcgccggaaa ccaccagatt ggggcggaat tgctccattt ttacactggc
    36061 cggacaacgt tgttggagat cacgtaacga ggcttcgtta gcaagaaggt aaggatagcc
    36121 atcagcaaat gacagaggta cagtgttgtg gcgtttcacg cgccgggtca tttgtggccc
    36181 cacccagcgt aattgcactt cgcgggagaa aaatccactt agccatttgt tgatcgcgtc
    36241 tggcgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt cttgtgtggc
    36301 gaaatcagca aaacgaacat atgcgctact gccatctggt gcggtgagat gcaagccatc
    36361 atgcacaggt gaaggagtaa accgtaccat ctggggaaac tggcgagcgg taataaacgt
    36421 accgtcaggt tccgtgatca taaagatgcg atcgaaggcc agaccactga catcagccag
    36481 agcatgtgta agaccaatgc cgcgcatcga tttaacagga tgaataaaaa gccgggttaa
    36541 tgtcaccacg gtgcggtcct caaatgaaaa taagccctca actttatgac atgacgcgct
    36601 tattagctat aatgcgcaac aattttctta gcgcctgtcc cattaggcta ttttatttgt
    36661 cagtttggcc ctgggttgtg ctcgaaatcc tcacgtactt gtgtacgctc cggtttctcc
    36721 gcgctgtcca tgtccagact gactgcaaca attacgccta ctgcggtagg ctcttagagt
    36781 aaaagtgacg atatgaattc tctgtttgcc agtacggccc gtgggctgga agagctgtta
    36841 aaaactgaac tggaaaacct gggggccgtt gaatgccagg tggttcaggg tggggtccat
    36901 ttcaagggcg acacacggct tgtttaccag agcctgatgt ggagccgcct ggcctcgcgt
    36961 attatgttgc cgctgggcga gtgtaaggtt tacagcgatt tagacctcta tctcggtgtt
    37021 caggcgatca actggacaga gatgtttaat cctggcgcga ccttcgctgt ccacttcagt
    37081 ggtttgaatg acaccatccg caacagtcag tacggcgcga tgaaagtgaa agacgcgatc
    37141 gtcgatgctt tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga tgcgccggat
    37201 atccgcgtta acgtctggct gcataaagaa accgccagta tcgctcttga tctgagtggt
    37261 gatggtttac atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat caaagaaacc
    37321 ctggcagccg ctattgtgat gcgatccggc tggcagccag gaacaccgct gctcgatccg
    37381 atgtgtggtt ccggtacgtt gctgattgaa gcggcgatgc tagcgaccga tcgcgcacca
    37441 ggcttgcacc gtgggcgttg gggctttagc ggctgggtgc agcatgatga agatatctgg
    37501 caggaagtga aagcggaagc gcaaactcgt gcccgtaaag gcctggctga gtatagctct
    37561 catttttacg gttcggacag cgacgcacgg gtgattcaac gtgcgcgcac taacgcccgt
    37621 cttgcgggga ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca actgaccaat
    37681 ccgctgccga aagggccgta cggcacagtg ttgagcaacc cgccatacgg ggaacgtctg
    37741 gacagcgaac cggcgctgat tgcgctgcat agcctgctgg gtcggatcat gaaaaaccag
    37801 tttggtggct ggaatctctc tttgtttagt gcctcgccgg atctgctaag ctgcttgcag
    37861 ctgcgtgcag acaaacagta caaggcgaaa aacggcccgc tggactgcgt acagaaaaat
    37921 taccacgttg ccgaaagcac tccagacagc aaaccggcga tggtagcgga agactacgcc
    37981 aaccgcctgc gtaagaacct caaaaaattc gagaagtggg ctcgccagga aggaattgaa
    38041 tgttaccgcc tgtatgacgc cgatctaccg gaatataacg ttgccgttga ccgttatgcc
    38101 gactgggtgg tggtgcagga gtatgcgccg ccaaaaacta ttgatgctca caaagcgcgt
    38161 cagcgtctgt tcgatattat cgctgcaacc atttcggtac tggggattgc gccaaacaaa
    38221 ctggtgctga aaacccgtga acgccagaag ggcaaaaatc agtaccagaa actgggcgag
    38281 aagggcgagt ttcttgaagt taccgaatat aacgctcact tgttggtgaa cctgacggat
    38341 tacctcgata ccggtctgtt tctcgatcac cgtatcgccc gtcgtatgct cggtcagatg
    38401 agcaaaggca aagatttcct caacctgttc tcttataccg gcagcgccac cgtgcatgcg
    38461 ggattaggcg gtgcacgcag caccaccacc gtggatatgt cgcgtactta tctggagtgg
    38521 gcagaacgca acctgcgttt gaatggcctg accgggcgtg cgcatcgcct gattcaggcc
    38581 gattgcctgg cgtggctgcg tgaggcaaat gaacagttcg atctgatctt tatcgatccg
    38641 ccaaccttct ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg cgatcatctg
    38701 gcgctgatga aagatttgaa acgtctgctg cgtgcaggtg ggacgatcat gttctcgaac
    38761 aataaacgtg gcttccgtat ggatctcgac ggcctggcaa aactgggact gaaagcacaa
    38821 gaaattacgc aaaaaacgct ctcccaggat ttcgcccgta accgccagat ccacaactgc
    38881 tggctgatta ccgcagccta aaggaatagt aatgtcatta atcagtatgc atggcgcatg
    38941 gctgtcgttc agcgacgcgc cgcttctcga taacgcagaa ctgcatatcg aagataacga
    39001 acgtgtttgt ctggtgggcc gcaacggcgc gggcaaatcg acgttaatga aaatcctcaa
    39061 ccgtgaacaa gggctggatg acggtcgcat tatttacgag caagatttga ttgtagctcg
    39121 tctgcaacag gatccgccgc gtaacgttga gggtagcgtt tatgatttcg ttgccgaagg
    39181 cattgaagaa caagcggaat atctgaaacg ctatcacgat atttcgcgcc tggtgatgaa
    39241 cgacccgagc gagaaaaatc tcaacgaact ggcgaaggtt caggaacagc tggatcacca
    39301 caacttgtgg cagctggaaa accgcatcaa cgaagtgctg gcgcaactgg ggttagatcc
    39361 taacgttgcg ctgtcgtcgc tttccggcgg ctggttgcgt aaagcggcat taggacgcgc
    39421 gctggtgagt aatccgcgcg tgctgttgct tgatgaaccg acaaaccacc tggatattga
    39481 aaccatcgac tggctggaag ggtttttgaa aactttcaac gggacgatta ttttcatctc
    39541 ccacgaccgt tcgtttatcc gcaatatggc gacgcgcatt gttgatctcg atcgcggcaa
    39601 gctggtgacc tatccaggga attacgacca gtacctgctg gaaaaagaag aagccctgcg
    39661 cgtggaagaa ttacaaaatg ccgagttcga tcgcaaactg gcgcaggaag aggtgtggat
    39721 ccgccagggg atcaaagcac gccgtacccg taatgaaggc cgcgtacgcg ccctgaaagc
    39781 gatgcgtcgc gagcgtggtg aacgtcgcga agtgatgggt accgcaaaga tgcaggtgga
    39841 agaggccagc cgctccggta agatcgtttt cgaaatggaa gacgtttgct accaggttga
    39901 cggtaagcaa ctggtgaaag atttttcggc ccaggttcta cgtggcgaca aaattgccct
    39961 gattggtccg aatgggtgcg gcaaaaccac gctgctgaaa ctgatgctcg gtcagcttca
    40021 agcggacagc gggcgtattc acgttggcac caaactggaa gtggcttatt tcgatcagca
    40081 ccgcgcggaa ctggatcccg ataaaacggt gatggataac cttgccgaag gtaagcaaga
    40141 ggtgatggtt aacggcaagc cacgccacgt attgggctat ttgcaggact ttctgttcca
    40201 tccgaaacgg gcgatgacgc cggtacgtgc gctttctggc ggtgagcgga accgcttgct
    40261 gctggcgcgt ttgttcctca aaccaagcaa cttattgatt cttgacgaac cgaccaacga
    40321 tcttgatgtc gaaacgctgg aactgctgga agaactgatc gacagctatc agggcacggt
    40381 attgctggtt agccacgatc gtcagtttgt cgataacacc gttacagaat gttggatctt
    40441 cgaaggcggc ggtaaaattg gtcgttatgt cggcggttat catgatgccc gtggtcagca
    40501 agagcagtat gtggcgctca aacagcctgc ggtgaaaaaa accgaagaag ccgccgcggc
    40561 aaaagcagaa actgtaaaac gcagcagtag caaactaagc tataaattgc agcgcgaact
    40621 ggagcagcta ccgcaattgc tcgaagatct ggaggcgaag ctggaagccc tacagacgca
    40681 agtggcggat gcttccttct tcagtcagcc gcatgagcag acgcaaaaag tgcttgctga
    40741 tatggctgct gcagagcagg agctggagca agcctttgaa cgctgggagt atcttgaagc
    40801 gttaaaaaat ggtggctgat cgcaaaatag tcgatttaaa cagagacagc tgacaacggt
    40861 aaaattgcct gatgaactgg gtttatcagg catgaaaacc aacactttgt tagtcatctg
    40921 taaaatagcg catcattaag gagtaccaat gtgcgaacat catcatgccg cgaagcacat
    40981 cctgtgctcg cagtgtgaca tgctggtggc gttaccgcgc cttgagcatg gtcagaaagc
    41041 ggcatgtccc cggtgtggca caacgttaac cgtggcgtgg gatgcccctc ggcagcgtcc
    41101 caccgcctat gcgttggctg cactgttcat gctgttgctg tccaacttgt ttccttttgt
    41161 gaatatgaac gttgcaggag ttaccagtga aattacatta ctggaaattc ccggcgtgct
    41221 tttttctgag gactacgcca gcctcggcac ctttttcctg ttgtttgtgc aactggttcc
    41281 cgcgttttgt ctgataacca ttctgttact ggtgaatcgc gcggaattac cggtccgttt
    41341 aaaagagcaa ctggcacggg tgctttttca actcaaaacc tggggaatgg cggagatttt
    41401 cctcgctggt gtgctggtca gtttcgttaa actgatggct tacggcagca ttggggtagg
    41461 cagcagcttt ctcccctggt gtttattttg tgtcctgcaa ctgcgcgctt ttcagtgcgt
    41521 tgatcgtcgc tggttatggg acgacatcgc cccgatgcca gaactgcgcc agccgctaaa
    41581 accaggcgtc acggggatac gtcaggggct gcgttcgtgc tcctgttgta cggcaatcct
    41641 tcctgctgat gaacccgtgt gcccgcgttg tagtaccaaa gggtacgttc ggcgtagaaa
    41701 cagcctgcag tggacactcg cgctgcttgt aacgtccatc atgctgtatc ttccggctaa
    41761 tattttgccc atcatggtga cggatttatt aggctcgaag atgccgtcga cgattctcgc
    41821 tggggtcatt ctgttatgga gcgaaggctc ttatcccgtc gctgcggtga tctttctggc
    41881 cagtattatg gtgccaacgt taaagatgat cgccatcgcg tggctgtgtt gggatgccaa
    41941 agggcatggc aagcgcgaca gtgaaagaat gcatttgatt tatgaagttg ttgagtttgt
    42001 aggccgctgg tcgatgattg acgttttcgt tatcgcggtg ctctcggcgc tggtgcgtat
    42061 gggaggttta atgagtattt atccggcaat gggtgcatta atgtttgctt tagtcgtcat
    42121 aatgacaatg ttttctgcta tgacgtttga cccgcgtttg tcgtgggatc gtcaacctga
    42181 atcagagcat gaggagtcct gacagtatgg aatctaataa tggggaagcc aaaatccaga
    42241 aagtgaagaa ctggtctccc gtgtggatat ttcctatcgt cacggcgctc attggggcct
    42301 gggttctttt ttatcattac agccatcagg gaccggaagt gaccctgatc accgcgaatg
    42361 cggaaggaat tgaaggtggc aaaaccacca ttaaaagccg tagcgttgac gtcggcgtgg
    42421 ttgaaagcgc cacactggct gatgatttga cgcacgttga aatcaaagcg cggctgaatt
    42481 ccggtatgga aaaattgctg cataaagaca ccgtcttttg ggtggtgaaa ccgcagattg
    42541 gtcgcgaagg gattagcggc ctgggaacgc tgctgtctgg agtttatatc gaactgcagc
    42601 caggcgcgaa aggcagcaaa atggataaat acgatttgct ggactcgcca ccgttggccc
    42661 cgcctgatgc gaaaggtatc cgtgtgattc tcgatagcaa aaaagccggg cagctctcgc
    42721 caggagatcc ggtgctgttc cgtggctatc gggtaggttc ggttgaaacc agcaccttcg
    42781 atacacaaaa acgcaatatc agctatcaac tgttcatcaa tgcaccttat gaccgactgg
    42841 tgaccaacaa tgttcgcttc tggaaagata gtggcattgc ggttgatctg acgtcagcag
    42901 ggatgcgtgt ggagatgggc tcattgacaa cgctgctgag tggcggtgtc agctttgatg
    42961 tgccggaagg tctggattta gggcagccag tggcaccgaa aacagctttc gttttgtatg
    43021 atgatcagaa gagcattcag gattcgttgt acaccgatca cattgattat ctgatgttct
    43081 ttaaagattc ggtacgcggt ctgcaaccgg gagctccggt agagttccgg ggtattcgcc
    43141 tgggtaccgt aagcaaagtg ccattctttg cgccgaatat gcgtcagaca tttaacgatg
    43201 attaccgtat tccggtactg attcgtatcg agccagagcg gctgaaaatg cagcttggcg
    43261 aaaatgcgga tgttgttgag caccttggcg aattgttgaa acgtggttta cgcggatcgc
    43321 tgaaaaccgg aaacctggtc actggtgcac tgtatgttga tctcgatttc tatccaaata
    43381 cgcctgcaat aaccggtatt cgtgaattta atggttatca gattatcccg accgttagcg
    43441 gcggcctggc gcaaatccag caacgactga tggaagcgtt ggataagatc aacaaactgc
    43501 cattgaatcc gatgattgaa caggcaacca gtacgctttc tgaaagtcag cgcacaatga
    43561 aaaacctgca aacgacgctg gatagcatga acaagatcct cgctagccag tcgatgcagc
    43621 agttgccgac ggatatgcag tcaacgttgc gtgaattgaa tcgcagcatg cagggcttcc
    43681 agcctggctc cgcagcctac aacaagatgg tggcggatat gcagcgcctt gatcaggtgt
    43741 tgcgagaact gcaaccggtg ctgaaaacgc tcaatgagaa gagtaacgcg ctggtatttg
    43801 aagcgaagga caaaaaagat ccagagccga agagggcgaa acaatgaaaa agtggctagt
    43861 gacgattgca gcactgtggc tggtcggatg cagctccggc gaaattaata aaaactatta
    43921 ccagttacct gtggtgcaga gcggtacaca aagtaccgcc agccagggca atcgtctgtt
    43981 atgggtagag caggtcactg ttcctgacta tctggcgggg aatggtgtgg tttatcaaac
    44041 cagtgatgtg aagtatgtga ttgccaacaa caacttgtgg gccagcccgt tggatcaaca
    44101 gttgcgcaac accctggttg ccaacctgag tacgcaactg cccggctggg tggttgcctc
    44161 ccagcctctg ggaagcgccc aggacacgct caatgttacc gtaacggagt ttaacggtcg
    44221 ctatgatggc aaggtcattg tcagtggtga gtggctgttg aaccaccagg gacaactgat
    44281 caaacgtccg ttccgtctgg aaggagtgca aactcaggat ggttacgatg agatggttaa
    44341 agtgctggcc ggtgtctgga gtcaggaagc cgcttctatt gcacaagaga taaagcgtct
    44401 accttaatta taaagatttg taaatataac cgtctccggt atgttgcctg aggcggtttt
    44461 tttgtttcta acgtgcggaa aaatttgttc ctcttcacat tttttgtaca accgacatgc
    44521 ccgtgtagct cacaaatatg acagtggcgt gaattttgcg cattgacggc agttatgatt
    44581 cgcggtattg cttaactgtg attgcacatt tagtaatcac tgttttcttt tccaccagaa
    44641 accagtatga gggaaacgag gcatgaagag acaaaaacga gatcgcctgg aacgggcaca
    44701 tcaacgtggt tatcaggccg gcatcgccgg acgctcaaaa gaaatgtgtc cctatcagac
    44761 gctgaatcaa aggtcacaat ggctgggagg ctggcgagaa gccatggcgg acagggtagt
    44821 aatggcctga ttctgtctct ttaaaaagaa acctccgcat tgcggaggtt tcgccttttg
    44881 atactctgtc tgattataat cagaaggcag acgtatcctg gaacagaccg actttcaggt
    44941 cgctggcggt atagataaga cgaccatcaa ccagcacttc gccatccgcc aggcccataa
    45001 tcagacgacg gttaacaatg cgtttaaagt gaatacggta ggtcactttt ttcgctgtcg
    45061 gcagtacctg accagtgaat ttcacttcgc caacgcccag cgcgcggcct ttaccttcgc
    45121 cgcccagcca gccgaggtag aaccctacca gctgccacat tgcgtccagg cccaggcatc
    45181 ccggcataac cggatcgcca ataaagtggc atccgaagaa ccacagatcc ggattgatat
    45241 ccagttctgc ttcaacatac cctttgtcga agttaccacc cgtttcggtc attttgacca
    45301 cacggtccat catcagcatg ttcggtgctg gcaattgcgg gcctttagcg ccaaacagtt
    45361 caccgcgacc agaggcaaga aggtcttctt ttgtatagga ttcgcgttta tctaccatgt
    45421 tctctgtaag ccttatttta ttgaagcacg caggatagct aacacgtgta cgctgaacaa
    45481 gtccgatcag ttcggaataa accagttcag ccaacgtaat ggccatggaa aacggtgacg
    45541 tccttcctgt tgcgatgctt gcgcgatacg ttcctggatg gtttgcatca gcgtcgtttg
    45601 gccttcgcca tcccacacca gatttaataa taacggcagt gcgtcagtca catcgtctac
    45661 tgcccagatg gtgaatttgc cttcttctac cgctttcacc agttcactgt gaagacttaa
    45721 atggcgaacg ttagctgtgg ggataatgac accttgtttc ccggttaact cacgttgctg
    45781 gcaaatagca aagaagcctt cgattttctc atttaaacca ccgaccggct gggcgcgacc
    45841 gaactgatcg actgaacctg tgatagcgat actctgattc accggcacat cggcgagggc
    45901 gcttatcagg gcgcagagtt cagccatcga ggcactatct ccatcaactt cactgtatga
    45961 ctgctcaaat gtcagcgatg ctgagaaggg gatctgttgc tcaagctgta gttccgacat
    46021 caggaacgct tgcatgatca tcatcccttt cgcatggata ttgccgccaa gctccgcttt
    46081 gcgttcgatg tcggtgaatt caccatcgcc aatatgcaca acgcagctaa tgcgagaagg
    46141 ttcgccaaaa gcgcgtggat gacccggaaa ttcaatgacc gaaagggcgt taatttgccc
    46201 gatgcgttcg ccttcggttt caatcaggat ttgctcctga aggatctcat cctgcatacg
    46261 ttcagcgagg aaaccttcgc gccattcacg ctgctgcagc attaagttta gctgctcgcc
    46321 ggagaaggtg tcgccatcac acagggaggc gacctcttta cactggcgga ggatccactg
    46381 cgggctaagc ggaagtgttt cttgttcacc ggtgtagcgt gctgcttcgc ggataagtac
    46441 cggccaggca tccgctcccg gtgcaggtaa gtgattatgt ctggcggtaa atgtcaccca
    46501 gcgacaccac tgggttactg actctgcatc gacaatctgc agagtatctt caaattcgct
    46561 ataaatagcc tgctctgaaa gctctggctc catctcctgg aaatcagcca atgattcgcg
    46621 ttcgcctacc agaatgactt tcagcttcaa tggcatcgaa ggcacagaga cggggagagg
    46681 gcgcgactca tcaaacgcaa cccagtcaaa acgctcgcgg ttaacgatat ttttcagccg
    46741 catccacagc agaggttgcg ccagcagtgt acgcaaagag atgatgagaa taccgccatt
    46801 tgcctgatgc accagaccag gctgcagggt aatgtcgcca ttaaactggc gcaggcagcc
    46861 aaagagttgc tccgcttcta cccagtcggc agcgacaact tgcgttaaag tcgcaaaatt
    46921 atcatctgca ctcactgcgt gacgtaagcg gatggagtgg ccggaaacct catagtgacc
    46981 gcccaccagt tggcctgcat cgctttgtag cgtacgcgcg gcattggcaa taagattcag
    47041 atactcagac tcttccgggg ccttcgccag cataaaagag gaggatgctc gcgtatgcag
    47101 caactgctcc agtgcgaatt gcagtcgcgg ttgagtatca ctgaataaag gatcgttttc
    47161 gtcaatcaaa tgtggctgag caaatatttc ctgatagcta tcggtatcag gaaccaggtc
    47221 acgccatgca agtttcgtaa tggtcaaagt tgatgttttt tagtctgttg tcaaagccgc
    47281 gattataccg taaccggcac tacagcacac gtagaaagca ccgacaatac tcctggcatg
    47341 ggcgttaaag ctcacaggat ggagattctt ttcttcactg gcctaaaaag ctgatattct
    47401 gtaaagagtt acacggtaac attgagatcg ctatgaaata tcaacaactt gaaaatcttg
    47461 aaagcggttg gaaatggaag tatctggtga agaagcatcg cgaaggggag ttaatcaccc
    47521 gttacataga agccagtgcc gcccaggaag ccgtcgatgt gttgctctcg ctggaaaatg
    47581 aacccgtgct ggtaaatggc tggattgaca agcatatgaa tccggaactg gtcaatcgca
    47641 tgaagcaaac cattcgggcc agacggaagc gccattttaa tgcggaacac cagcatacgc
    47701 gcaaaaaatc gatcgatctg gaatttattg tctggcaacg tctggctggt cttgcgcagc
    47761 gtcgcggtaa aacgctttct gaaacgattg ttcagctgat tgaagatgcg gaaaacaaag
    47821 agaaatacgc gaataaaatg tcttctttga agcatgatct gcaggcattg ctgggtaagg
    47881 aataactgac gaagtcagtt caatttacta aaggcgaaaa aaaccccgca gctgcggggt
    47941 ttttctacca gacgagaact taagcctgcg gctgagttac aacgtctttg atgcctttaa
    48001 cttcgatctc tacgcgacga tccggagcca ggcagtcgat cagtgcagca cgctgtttca
    48061 cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc cataccacgt gcggagatct
    48121 tgtctgccgg gatacctttg gagatcaggt aatcaacaac agactgagca cggcgctcgg
    48181 acagaccctg gttgtaagcg tcagaaccga tgcggtcagt gtaacccaga acaactacgg
    48241 aaccgtcttt cggatccagg ttgctcagct ggctgtacag ctgatccaga gcagcctgac
    48301 cttccggttt cagggttgct ttgttgaagt tgaacagaac gtcagacttc agagtgaagt
    48361 gcttggtctg tacttccggt gccggagctg gagccggagc aactaccgga gctgcttcgc
    48421 cctgaccgaa acggtaggaa acacccaggc tcagcagacc gttgtccgga cgagtaccga
    48481 tggtgtttgc gtcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcag
    48541 gagtgatcgc gtactcaaca ccacctgcga aaaccggaga aacgccagtg tcgtggtctt
    48601 taaaggatgc gccaccaggt acgttagcct tggtgtctgc acgccatacc ataccaccca
    48661 gacgagtgta tacgtccaga tcgtcagtga ttgggtaacc cagtttagca gtcagttgaa
    48721 cgccctgagc tttgtatgca ccgttgatgt tgtcgccttt gtacggcata cgacctaacc
    48781 agtcgtaacc catttcaaaa ccaacatacg ggttaacctg gtaaccacca aaagcacctg
    48841 cacccagttg gttttcgtgg gtcggaccat tgttaggaat aaaaccagtg tcatggtact
    48901 gggaccagcc cagtttagca ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta
    48961 cggtagcgaa accagccagt gccactgcaa tcgcgatagc tgtctttttc attttttgcg
    49021 cctcgttatc atccaaaata cgccatgaat atctccaacg agataacacg gttaaatcct
    49081 tcaccggggg atctgctcaa tattaactct accgatatct tcggcttatg ccgagcaccc
    49141 ctggcgatgt aaagtctaca acgtagttga aaacttacaa gtgtgaactc cgtcaggcat
    49201 atgaaaaaaa agtcttgtat aaggtatgtt taatcttttt tgtcagcgac aatttacaga
    49261 agaaaatcgc ggaaaccgct tcagacaagc ctccgcaagg aaaattagtc acgactgaaa
    49321 gcattggctg ggcgacaaaa aaagttccag gattaatcct aaatttactt aatgatacaa
    49381 attagagtga atttttagcc cggaaagttg tctcgtggcg tgagaggatg cgcttaccgg
    49441 acgcataata aaccccatag cgttaccttc atttgccgca tcaacaagtt cagcatgctc
    49501 ttcttcagtc aaatcatctg ccaaccaacc gatcaccaca ctgtaattgc ccgtgcgtaa
    49561 agcgcgaacc attgactcca cagtgtggca aggggagagc tggctaatct gcattacttt
    49621 cgttaagggt agcccagatg cctgaaccca ttcccgactc agtttttgtt gcggtgttaa
    49681 ccagagttgc cagcgcgatt gctgaccgag ttgctgtaac aatggcaaca gtagaagttg
    49741 cgtcatcatg ggctgatctt cgcgatagac aacttcactg ataagcccgg ctgtagtgtt
    49801 ttccgtagag acacgcgcaa ttttacttgc tgcggatgag aacgacgaag aacgatgtgc
    49861 atagcctgaa gtgtacataa tcaatccagc ccctgtgagt tactgtatgg atatacagta
    49921 catccagtga caacaaagat caaccctatt ttcggaaaga gcctcgcaaa ttttgtcgtt
    49981 ggtgacggga aaacataaat taatcttgcc ccttaagaat aagttgccta ttttcgtagt
    50041 taacggatcc gttaatgtga atcattcttt tatgttatga ttttaaaagg aattttatga
    50101 aaagcctctc ctataagcgg atctataaat cacaagaata cctggcaacg ttgggcacaa
    50161 ttgaataccg ctcattgttt ggcagttaca gcctgaccgt tgacgacacg gtgtttgcga
    50221 tggtttctga tggtgagttg tatcttcggg cttgtgagca aagtgcacag tactgtgtaa
    50281 aacatccgcc tgtctggctg acatataaaa agtgtggccg atccgttacc ctcaattact
    50341 atcgggttga tgaaagtcta tggcgaaatc aactgaagct ggtgcgtctg tcgaaatatt
    50401 ctctcgatgc agcgctgaaa gagaaaagca cgcgcaatac ccgggaaaga ctgaaagatt
    50461 tgcccaatat gtcttttcat ctggaagcga ttctcgggga ggtggggatt aaggatgtac
    50521 gggcgttacg tatacttggg gcaaaaatgt gttggttgcg actgcggcag caaaacagtc
    50581 tggtgacaga aaagattctg tttatgcttg aaggtgccat tatcggcatt catgaagctg
    50641 cgctcccggt ggcacgccgc caggagcttg cagaatgggc tgactctctt acgccgaaac
    50701 aggagtttcc tgcggaactt gagtaatctg gcgttgcaga cgaccaatct cag
//
LOCUS       DAEVZA010000020        50237 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEVZA010000020 DAEVZA010000000
VERSION     DAEVZA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.020"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            462..647
                     /locus_tag="LAF92_001190"
     CDS             462..647
                     /locus_tag="LAF92_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790235.1"
                     /translation="MTIEERLNNIELNQTLLDQRLSELELKDLDAQISEAEAKLSSLN
                     HRKKQIRNRITQGRGSC"
     gene            complement(725..1261)
                     /locus_tag="LAF92_001191"
     CDS             complement(725..1261)
                     /locus_tag="LAF92_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBI9790236.1"
                     /translation="MLHTIHFLCPVNTATVGQLQNHCLTALSQGATELNIHISSQGGE
                     TAAGFTAYNFLKSLPVAVRTHNISNVESIANIVFLAGSERFANPLSRFLLHPLLWGFA
                     TPAADHARLREYGKCLDNDLDRFVETFNIDIGTHIRWASLIADSTILDANKALEHGII
                     NSIKTARLVSNQANWWVV"
     gene            complement(1261..1497)
                     /locus_tag="LAF92_001192"
     CDS             complement(1261..1497)
                     /locus_tag="LAF92_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBI9790237.1"
                     /translation="MSREEPQINIRISKELKAKVKARAQHNKRSMNAEIIQIIEDAVC
                     GRSLNSNEFAQKEADKFRDALLETLKTMYGKDEK"
     gene            1586..1759
                     /locus_tag="LAF92_001193"
     CDS             1586..1759
                     /locus_tag="LAF92_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBI9790238.1"
                     /translation="MIVKSDAPKYPLRIPLEVKLAIEKSAKENGRSINTEMVMRLVDS
                     LRRDSSKGNLAKS"
     gene            1822..2700
                     /locus_tag="LAF92_001194"
     CDS             1822..2700
                     /locus_tag="LAF92_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HBI9790239.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALTLT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRYDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKKTDELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSVHNY"
     gene            2801..4855
                     /locus_tag="LAF92_001195"
     CDS             2801..4855
                     /locus_tag="LAF92_001195"
                     /inference="COORDINATES: protein motif:HMM:NF024627.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HBI9790240.1"
                     /translation="MTDITANVVVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NRIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLGYEPDQFQVNIGSSDGFKYVGQCPDVPTLRTIEPTTNGQRILLREYTAGTGYG
                     GGQLRAVLDGSSYTDNGVTVFKTPGGAAWIRVNADIINPLMAGAVPDGVTPCSAAINA
                     AFTAAKRISFPDGDYLLDATITAHEARRIDFGAARLIQGATLANYIFAIRGSNTQARG
                     GNIVGTGIRTGASSVVSAVLVADTSHSSVKGMTVRRVPGSGIQMINCTRCKIQDCISE
                     NNWGMGLEDRYGTFNEVINSISNSNGYLSDTEINATGRGCIVWKSTGTIFKGNQTFGN
                     SEYGIRVYSQSGDDAPTRYIHIIDHIAGDNQKTDIYVYNDSGNLLGIELTNCHVHRWN
                     QSFSGSPMVALQGQRCHFNGGSLTKYGDIYDSRAIQCSNGDNITVENVSINNVTHAFG
                     NSANNTIFRNCTTYSSTITDASGGTTGVAKYEGIDAVHAGTGTNDYAITVTAACAITN
                     VTFLGFARNISWNNENLTIRDCRSFNTTGDAIRMYGDGVGALQMSGNRWDVSTTPAWL
                     GSLVKQGSALPVLYDTAAPTTLTWPRGSKCINSAPTVGRPKGWVCTASGTPGTWVAES
                     NL"
     gene            complement(5070..6227)
                     /gene="intS"
                     /locus_tag="LAF92_001196"
     CDS             complement(5070..6227)
                     /gene="intS"
                     /locus_tag="LAF92_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBI9790241.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLRSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(6389..6463)
                     /locus_tag="LAF92_001197"
     tRNA            complement(6389..6463)
                     /locus_tag="LAF92_001197"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6428..6430),aa:Arg,seq:cct)
     gene            complement(6539..7471)
                     /locus_tag="LAF92_001198"
     CDS             complement(6539..7471)
                     /locus_tag="LAF92_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBI9790242.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            7766..8521
                     /gene="mlaA"
                     /locus_tag="LAF92_001199"
     CDS             7766..8521
                     /gene="mlaA"
                     /locus_tag="LAF92_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBI9790243.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(8703..9761)
                     /locus_tag="LAF92_001200"
     CDS             complement(8703..9761)
                     /locus_tag="LAF92_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790244.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTE
                     YQNEINQLSAPGVNFDDI"
     gene            complement(10127..11467)
                     /gene="fadL"
                     /locus_tag="LAF92_001201"
     CDS             complement(10127..11467)
                     /gene="fadL"
                     /locus_tag="LAF92_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBI9790245.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            11839..12123
                     /locus_tag="LAF92_001202"
     CDS             11839..12123
                     /locus_tag="LAF92_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBI9790246.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            12303..13613
                     /gene="fadI"
                     /locus_tag="LAF92_001203"
     CDS             12303..13613
                     /gene="fadI"
                     /locus_tag="LAF92_001203"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBI9790247.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            13613..15754
                     /gene="fadJ"
                     /locus_tag="LAF92_001204"
     CDS             13613..15754
                     /gene="fadJ"
                     /locus_tag="LAF92_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBI9790248.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDL"
     gene            15960..16445
                     /gene="sixA"
                     /locus_tag="LAF92_001205"
     CDS             15960..16445
                     /gene="sixA"
                     /locus_tag="LAF92_001205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBI9790249.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            17129..17692
                     /locus_tag="LAF92_001206"
     CDS             17129..17692
                     /locus_tag="LAF92_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI9790250.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            17774..20419
                     /gene="yfcU"
                     /locus_tag="LAF92_001207"
     CDS             17774..20419
                     /gene="yfcU"
                     /locus_tag="LAF92_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HBI9790251.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVANNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            20439..21191
                     /locus_tag="LAF92_001208"
     CDS             20439..21191
                     /locus_tag="LAF92_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI9790252.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            21207..21716
                     /locus_tag="LAF92_001209"
     CDS             21207..21716
                     /locus_tag="LAF92_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9790253.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            21713..22201
                     /locus_tag="LAF92_001210"
     CDS             21713..22201
                     /locus_tag="LAF92_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9790254.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            22198..22737
                     /locus_tag="LAF92_001211"
     CDS             22198..22737
                     /locus_tag="LAF92_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9790255.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGAQAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVSGQSAHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            22739..23593
                     /locus_tag="LAF92_001212"
     CDS             22739..23593
                     /locus_tag="LAF92_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBI9790256.1"
                     /translation="MKILRHVLCLLLLLMFNTCGQAATKVGELYVPAGASSPATEFEF
                     HLIIATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSRVSMSACPGLEQ
                     YDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDDDFPDYIGPTKV
                     SSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCNGNNFDE
                     RGAYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCR
                     FTYILNMY"
     gene            complement(23666..24217)
                     /gene="smrB"
                     /locus_tag="LAF92_001213"
     CDS             complement(23666..24217)
                     /gene="smrB"
                     /locus_tag="LAF92_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBI9790257.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGSRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            24383..25315
                     /gene="prmB"
                     /locus_tag="LAF92_001214"
     CDS             24383..25315
                     /gene="prmB"
                     /locus_tag="LAF92_001214"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBI9790258.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            25350..26435
                     /gene="aroC"
                     /locus_tag="LAF92_001215"
     CDS             25350..26435
                     /gene="aroC"
                     /locus_tag="LAF92_001215"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBI9790259.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            26439..27263
                     /gene="mepA"
                     /locus_tag="LAF92_001216"
     CDS             26439..27263
                     /gene="mepA"
                     /locus_tag="LAF92_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBI9790260.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDLRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            27263..28072
                     /locus_tag="LAF92_001217"
     CDS             27263..28072
                     /locus_tag="LAF92_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBI9790261.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            28072..28620
                     /gene="epmC"
                     /locus_tag="LAF92_001218"
     CDS             28072..28620
                     /gene="epmC"
                     /locus_tag="LAF92_001218"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBI9790262.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            28654..28932
                     /locus_tag="LAF92_001219"
     CDS             28654..28932
                     /locus_tag="LAF92_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBI9790263.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(29053..31059)
                     /gene="mnmC"
                     /locus_tag="LAF92_001220"
     CDS             complement(29053..31059)
                     /gene="mnmC"
                     /locus_tag="LAF92_001220"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBI9790264.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWNEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSHTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            31218..32438
                     /gene="fabB"
                     /locus_tag="LAF92_001221"
     CDS             31218..32438
                     /gene="fabB"
                     /locus_tag="LAF92_001221"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBI9790265.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            32749..33927
                     /locus_tag="LAF92_001222"
     CDS             32749..33927
                     /locus_tag="LAF92_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9790266.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIVVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33924..34919)
                     /gene="flk"
                     /locus_tag="LAF92_001223"
     CDS             complement(33924..34919)
                     /gene="flk"
                     /locus_tag="LAF92_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBI9790267.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            35018..36154
                     /gene="pdxB"
                     /locus_tag="LAF92_001224"
     CDS             35018..36154
                     /gene="pdxB"
                     /locus_tag="LAF92_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBI9790268.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            36220..37233
                     /locus_tag="LAF92_001225"
     CDS             36220..37233
                     /locus_tag="LAF92_001225"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBI9790269.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            37233..38045
                     /gene="truA"
                     /locus_tag="LAF92_001226"
     CDS             37233..38045
                     /gene="truA"
                     /locus_tag="LAF92_001226"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBI9790270.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            38128..38787
                     /locus_tag="LAF92_001227"
     CDS             38128..38787
                     /locus_tag="LAF92_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI9790271.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            38943..39857
                     /gene="accD"
                     /locus_tag="LAF92_001228"
     CDS             38943..39857
                     /gene="accD"
                     /locus_tag="LAF92_001228"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBI9790272.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            39927..41195
                     /gene="folC"
                     /locus_tag="LAF92_001229"
     CDS             39927..41195
                     /gene="folC"
                     /locus_tag="LAF92_001229"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBI9790273.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGILH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            41185..41847
                     /gene="dedD"
                     /locus_tag="LAF92_001230"
     CDS             41185..41847
                     /gene="dedD"
                     /locus_tag="LAF92_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBI9790274.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            42106..42594
                     /gene="cvpA"
                     /locus_tag="LAF92_001231"
     CDS             42106..42594
                     /gene="cvpA"
                     /locus_tag="LAF92_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBI9790275.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            42631..44148
                     /gene="purF"
                     /locus_tag="LAF92_001232"
     CDS             42631..44148
                     /gene="purF"
                     /locus_tag="LAF92_001232"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBI9790276.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            44243..44812
                     /gene="ubiX"
                     /locus_tag="LAF92_001233"
     CDS             44243..44812
                     /gene="ubiX"
                     /locus_tag="LAF92_001233"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBI9790277.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            45078..45860
                     /gene="argT"
                     /locus_tag="LAF92_001234"
     CDS             45078..45860
                     /gene="argT"
                     /locus_tag="LAF92_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBI9790278.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            46081..46863
                     /gene="hisJ"
                     /locus_tag="LAF92_001235"
     CDS             46081..46863
                     /gene="hisJ"
                     /locus_tag="LAF92_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBI9790279.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            46953..47639
                     /gene="hisQ"
                     /locus_tag="LAF92_001236"
     CDS             46953..47639
                     /gene="hisQ"
                     /locus_tag="LAF92_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBI9790280.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            47636..48352
                     /locus_tag="LAF92_001237"
     CDS             47636..48352
                     /locus_tag="LAF92_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9790281.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGRVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            48360..49133
                     /gene="hisP"
                     /locus_tag="LAF92_001238"
     CDS             48360..49133
                     /gene="hisP"
                     /locus_tag="LAF92_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBI9790282.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            49330..50232
                     /gene="rpnB"
                     /locus_tag="LAF92_001239"
     CDS             49330..50232
                     /gene="rpnB"
                     /locus_tag="LAF92_001239"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBI9790283.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT        12170 a        12175 c        13190 g        12702 t
ORIGIN      
        1 gagaatggtg ttttgcatgt gataggcgtg ttcaatggca aactatctac tattgcccat
       61 gagtgcgctc acatggcatt cgatatctgc tcaagggtcg gtgttgatgt tgaaccagga
      121 agatccaacg agacttactg ctacttaatg agcaggcttg ttgagttctg cgagcgacat
      181 atcaaaaagc cggagtgaac cggcttgatt attacttttt ttggtatgtt aagaatggca
      241 agtcgccagt gtaccccaag cggaaaagct ctaattgctt atctcttatg gataaaaaat
      301 aatcgtttct tgccttttta agccacaacc agccgatcat caagatgaat aatattgata
      361 ttattggcat cggggagatc aacaccactc cgatgaataa tgacgctagc agcaccgata
      421 aaatagcaga aatcatacta atctcccact aaggtaacaa tatgactata gaagaacgtc
      481 tgaacaacat tgagttaaac caaaccctgc ttgaccagcg actttcagag cttgaactta
      541 aagatctgga tgcgcaaata tcagaagcag aagccaagct ctccagccta aaccaccgca
      601 agaagcaaat ccgcaacaga attactcagg gacgcggaag ctgttgaggt gtgatggtaa
      661 tccttgctca ctaaatttaa ggatgcgaat ctttccggcc ataggagtaa tcatgattta
      721 cccatcaaac aacccaccag tttgcctgat tggataccag ccttgcagtt tttatggaat
      781 taattatgcc atgctcaaga gccttgttag catccaaaat ggtcgagtct gctatcaggg
      841 atgcccacct aatatgggtt ccgatgtcga tattgaacgt ctcaacgaag cgatcaagat
      901 cgttatcgag gcatttcccg tactctctca atctggcatg gtcggcggct ggggtggcaa
      961 agccccataa tagaggatgt aacaggaatc ttgataatgg gtttgcgaaa cgttctgagc
     1021 cagctaggaa aacgatatta gctatggatt caacattgct tatgttgtga gttctaacgg
     1081 caacagggag tgacttaaga aagttatacg cagtaaaacc agcggcagtt tcccctccct
     1141 gacttgatat atggatattt aattcagttg cgccttgaga taatgcggtg agacagtggt
     1201 tctgaagttg cccaacagtg gcagtgttaa cggggcataa gaaatgaatt gtgtgcagca
     1261 ttatttttca tccttaccat acatggtctt tagcgtctca agcagcgcat ccctgaattt
     1321 gtcagcctct ttctgagcaa attcattgct attaagcgat ctaccacaaa cagcatcttc
     1381 gataatctgt attatttcag cattcatgga acgcttgtta tgttgcgccc tggctttaac
     1441 ctttgccttt aactctttgg aaatcctaat atttatttgc ggctcttcgc gtgacatacc
     1501 acctccatag cattttggtg atattactat tgcatcactg cgatcacaat ggtataacgg
     1561 ttataccaaa ttgattggag gtaatatgat agtcaagtca gacgcaccaa agtacccttt
     1621 gcgcatccca ttagaggtta agttagcaat cgagaagtca gcgaaagaaa atggtcgctc
     1681 aataaatacc gagatggtaa tgcggttggt ggatagttta aggcgggata gttctaaagg
     1741 taatctagca aaaagttgaa gccccaactg cgggaacagt cagggcttcg gtatcaacaa
     1801 atcggattag gaaatattga catgaaaagt atagcaaagg cacaaaacga tttcaccatc
     1861 ttcaaattcg gcgacagtga aatccgcgtc atcaacaaat gcggtgagcc gtggtttgta
     1921 gcaaaagatg tttgtgatgc tttaaccctg actaactcac gcaaggcgct tactgcactt
     1981 gatgacgatg aaaagggagt aactttaagt tacacccttg gtggtgagca gaatctaagc
     2041 attgttagcg aatcaggtat gtatacattg gttctgcgct gccgcgatgc tgtcaataaa
     2101 ggttcggtcc cgcacaaatt ccgcaagtgg gtaacagcag aagttctgcc ttcaattcgc
     2161 aaacatggcg agtatgtgaa aggcaagaaa accactgttg aggaaagaac accgctacgc
     2221 gatgcagtaa acatgctggt aggaaagaaa ggacttcgct atgacgatgc atacaatatg
     2281 gttcatcagc gttttggtat tgacagcatt gatgaacttt caattgaaca aatcccgctg
     2341 gccgtagagt acatccacag ggtagtgctt gaaggtgaat tcatcggaaa acaagagaag
     2401 aaaactgatg agctttctgc aaaagaagca aacagccttg tatggttatg ggattatgcc
     2461 aaccgctcac aggcattatt ccgcgaactg tatccggcgc taaaacaaat tcaatcgaac
     2521 tattccggca gatgctacga ctacggtcat gagttctcgt atgttatcgg aatggcgaga
     2581 gatgttttaa tcaaccatac acgagatgtt gatattaatg agccagacgg accaacgaat
     2641 ctttccgcat ggatgagact taagaataaa gaattacctc cttcagtaca taactactga
     2701 cagataacca acgcaactac ccagcttcgg ctgggttttt ttatgcccaa aattcaccgt
     2761 agccatgctg cggcgattcc ttgtatctgg agcaaattaa atgacagata ttacagccaa
     2821 tgttgtagtg agtatgcctt cgcaactctt cactatggcg cgttctttta aagccgtagc
     2881 caatggtaaa atttatatcg gtaaaattga cacggacccg gtaaatcctg aaaaccggat
     2941 tcaggtttat gtagagaatg aagacggttc tcacgttcct gtttctcaac caatcatcat
     3001 taacgctgct ggttatccgg tatataacgg acagattgcc aagttcgtaa ctgtgcaagg
     3061 ccattctatg gctgtttatg atgcgtatgg tgcacagcag ttttatttcc caaatgtact
     3121 tggatacgag ccagaccagt ttcaggtcaa tattggttcc agtgatggat ttaagtatgt
     3181 agggcagtgt cctgacgtac ccactcttag gacaatagag ccaaccacaa atggacagcg
     3241 cattctcctt cgtgaataca cggcaggtac aggatacggt ggcggtcagt tacgagcagt
     3301 gctcgacggt agctcctaca ccgacaacgg cgttactgta ttcaagactc ctggtggagc
     3361 ggcatggatt cgtgttaatg ccgatatcat aaacccgtta atggctggtg ctgtacctga
     3421 cggcgttacg ccgtgttcag cggcaattaa cgccgcattc acggcagcta aacgaattag
     3481 tttccctgat ggcgattacc tgctggacgc aacaatcacg gcgcatgaag cccgccgtat
     3541 tgactttggc gcggcaaggt taatccaagg agctacgctt gctaactata ttttcgctat
     3601 acgtggttcg aatacacagg cgcgtggcgg gaatatcgtt ggtacaggta tcaggactgg
     3661 agcaagcagt gtagtgtctg ctgtgttagt tgccgatacc tctcatagct ctgttaaagg
     3721 aatgaccgtc agaagggtgc ctggctctgg cattcagatg atcaattgta ctcgttgcaa
     3781 gattcaggat tgcatatctg aaaataactg gggtatgggg cttgaagacc gttacggaac
     3841 attcaatgaa gttatcaatt caatcagcaa ttccaacgga tacctgagtg atacagagat
     3901 aaacgcaaca ggccgtggtt gcattgtctg gaagagtacc ggcacaatct tcaaaggaaa
     3961 ccagacattc ggcaacagcg aatatggtat tcgcgtttac tctcagagcg gtgatgatgc
     4021 tccgacaaga tacattcaca ttatcgacca cattgctggt gataaccaga agactgatat
     4081 ctatgtatac aacgattctg gtaatttgct tggcatcgaa ttaacaaact gccatgtaca
     4141 ccgatggaat caatctttca gcggtagtcc aatggtcgcc cttcagggac aaagatgtca
     4201 ctttaacggc ggaagcctga ctaaatacgg tgatatttac gactcgcgag ccattcagtg
     4261 cagtaatggc gataatatta ctgtagagaa tgtgagtatt aataatgtca cccatgcatt
     4321 tggtaactct gcgaataata ctattttcag aaactgtaca acgtactctt caacgataac
     4381 tgacgcgtct ggaggcacga caggggtagc caagtatgaa ggtatagacg cggtgcacgc
     4441 gggtactggt actaacgatt atgcgattac ggttactgct gcttgcgcta ttacaaacgt
     4501 cacatttctg ggatttgcgc gaaatatttc atggaacaac gagaacctaa ccatcaggga
     4561 ctgccgcagc tttaatacaa ccggtgatgc aatccgcatg tacggtgacg gtgttggcgc
     4621 attgcaaatg tctggcaacc gctgggatgt gagcacaaca ccggcatggc ttggtagcct
     4681 ggtgaaacaa ggtagcgcac tgccagttct gtacgacacc gcagcgccaa ccacgttgac
     4741 gtggcctcgt ggctcaaaat gcattaacag cgcaccaact gtaggtagac cgaaaggttg
     4801 ggtgtgcacc gcttccggaa ctcctgggac ctgggtagct gaaagcaacc tttaatcacc
     4861 ttgcgctgta cacagtgaaa taaccgtaag agtcaatctt ggtaaaccca ttaagcggct
     4921 tatccgacac tatgtggtta aagccgcttt tactcatctc cttatcaaac cgatcctgat
     4981 tacttactat tgttacctgt ttatcaggta ggcaatctgt aagcccatga ttgcaaggct
     5041 ttgtgatatt cgatagtggt taaggtcgat cactctacct ttccatcaag ccagtccgcc
     5101 caccactgca tcatttctct gcgcttatcg agatactgag catggttgta aatcccgcgc
     5161 acagaaccgc cgttggcatg tgccagttgc acttcaatgg cgtcggcagg ccattcgtgc
     5221 tcgttcataa tcgtgctgaa ttcatgcctg aatccgtgac cgctttccag accttcatag
     5281 ccgatttgtt tgatcacaag tagcaccgcg ttctcgcaga ttggcttttt cttatcgttg
     5341 cgcccggcaa aaacaaactc tgatactggt ttggtgattg atcttagcgt agtgagaagt
     5401 tcaaccacct ggtctgacat cgggaccaca tgaattttgc gtcccttcat cacactggcg
     5461 tcgatggtga taatcctgtt ttcaaaatcg acgttcttcc attgcatgga acgaagctct
     5521 ttcgttctta gggctgtgta gcgtaaaact ttggtcgcaa tgagcgatac gatacttcct
     5581 gaaaatgttg ccagtgcttt gttgaatgca ggcatctggt ctgctggaag gaacgggaag
     5641 ttcttcttgc ggtatccttt catggcgtct gcaaggtcag gtgccgggtt atatttagcc
     5701 cttccggtga caatagcgta acgaaaaacc tcgccgcatc ttctgcgggc tttgttggct
     5761 cgctccattg caccgcgatc ttcaaatctg cggattactt ccagcagttg catcggctca
     5821 atatcctgaa tctcaagacc gccgatgatg ggtaaaatgt cgtcatcaaa catttttgca
     5881 agttcagttg catagcctac tgaccagact tgcttcttgt gctcgtacca ttccttgtaa
     5941 atggcgctaa aggaattgtt gttagacgaa gcctttttcg cctttacagg atcgatgcca
     6001 accgagatgt ctttcctcgc agtccatgct ttatcccttg cctcctgcaa agtcataagc
     6061 ggatattttc cgacggtcag gattttctcc ttaccgtcaa tcttgtagcg aagctgccat
     6121 acctttttcc ctgacacagg gacataaagg tacaggccat taccatcgag caggcggtat
     6181 ggtttttctt tcggctttgc tgcttcaatc tgcttaacgg tgagcatggg taaaaatccg
     6241 gtgggtaaaa ttattttatc cactttttac ccgtcatgga gtgcggctgt caacgatctg
     6301 acgcgaacca ttacgaactg tgaatatacg gaaggcttga tattcagggg attttgcgga
     6361 ctggtacgga tgggagcgaa ctgataaatg gtgtcccctg caggaatcga acctgcaatt
     6421 agcccttagg aggggctcgt tatatccatt taactaagag gacaatgcgg catgagtata
     6481 cccgctaatg gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt
     6541 atgctgggtt tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt
     6601 ctgctttacg cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga
     6661 taaaggtgcc gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat
     6721 cgctccagtg cagcgtgccg ttaaacacca gatagaggat ttcaacagaa ccgacaacga
     6781 tgtgggtggt gtcacccagt gcaataagcc aggtcatcaa tataatcacc acaatctttg
     6841 ccgcacccgc agcaggaaac atccaaacca tagtggcgat cagccagccg gaaatgatcg
     6901 cgttggcaaa catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg
     6961 catcgcgagt ttcttcattg aagataggca tatattcaaa tgcccatgca gcaatacctg
     7021 tcccgagaat attacccagc agcacgacgc cccataatcg cataagtaag ccgacgttgc
     7081 tcattgtcgg tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt
     7141 gctggcgggc cataatgacg ataataaaac caaaggtata accgagattc tccagtaaga
     7201 agctgcctgg cactccttcc agttcgacat gaaatatccc ttttgccagt agcgaagcgc
     7261 ccatcgacag acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttccagct
     7321 ctttttcacc atcctggcgg atatgctcat gaattgccat cgcccgggag gggagtcgat
     7381 cttcatctat ttctattttt ttgccgcgct ctttttcttc gctctcaact tcaatttcgt
     7441 cgctgtgttg atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag
     7501 ctaaagcgta gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa
     7561 ggctgcgttt gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc
     7621 aggctatgat caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat
     7681 cgcccacacc taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag
     7741 ccataaaaaa aacagggaga catttatgaa gcttcgcctg tcggcgcttg ctctgggaac
     7801 tacgcttctg gtggggtgtg cgagttccgg tacagatcag caagggcgtt ctgacccgtt
     7861 agaagggttc aaccgcacca tgtacaactt caacttcaac gtattagacc cgtatattgt
     7921 tcgaccggtc gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta atggtttgag
     7981 caactttact ggcaaccttg aagaacctgc ggtgatggtt aactacttct tgcagggcga
     8041 cccttatcag gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg
     8101 cggctttatt gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg
     8161 cttcggtagt acgcttggtc attatggcgt gggttatggg ccttacgttc agttaccgtt
     8221 ctacggtagc ttcacgctgc gtgatgacgg tggtgatatg gcggatggtc tttacccggt
     8281 tctttcctgg ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac
     8341 ccgcgctcag ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc cttatattat
     8401 ggtgcgcgaa gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc
     8461 gcaggaaaat ccgaacgcac aagcgattca ggatgattta aaagatattg attctgaata
     8521 agaaacaaat aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta
     8581 ccacaagtaa aaggagataa accgacaatg acccggacgt aatgatgtaa aaatactgat
     8641 gttcgtcact gactatttcc gatattcagg agttgaaaaa tcaaaaaact ggaaaaataa
     8701 ttttaaatat catcaaaatt aacacctggc gcagatagtt gattaatttc attttgatat
     8761 tccgtctggc gattttgaag ccttttttca actttatctt cgagatcctt aaagtctttg
     8821 tatttatttt caggatggtc aacgtcaact ttgttagcga ataaagtaga aattatttct
     8881 tttacattat cgcgtctaaa ttttgcatcg tccacttgaa aattatatct tattgctcga
     8941 cctaaacgtt taaaaacttc gttaggttct ctcagtcttt ccccgttgag ctcgtttaca
     9001 acctgatgat cggaacctgt tgcaaatgat tctgccatga tagctttgct gctttcaaac
     9061 cacgctgaag cactcctggc gttttcacta caatattggg tgagactatc accattaata
     9121 cgaattcctc tttgaatgtg gtagtcgtcc tgatcgtagt aaccgagaga taagtaatca
     9181 tttccgtttt tatttttaat tacaatagcg agagagtcgc cttttttatc tttaccgtac
     9241 caggtaaata agtccgttgg ttcgctattc ccgctctcat caaattccgt taccgtatgt
     9301 ctgagtttgt gtgatatctt atcgaagata aaaccaggat ctccatcata tatttctttt
     9361 aatgcgctga agatcatttc cttgttattg atatcatgag tcttctcaac tatgttgtca
     9421 ttaatgatag tacggacgag ttctttgtct ttatagttcg caggtaattc tattggttct
     9481 tggttgtcga taccaatagt aatatggtct gtatcattgg agccaatatc aatataagta
     9541 actgaggaat tattaatatt aaatctataa ggatcgtcgt ttactcgtat ggcgtttttt
     9601 ataagctcaa gtagatgttt aatacaggtg cttattcctt ccttaccatt aaaaaggttt
     9661 ttgagctctt gtactaatcc attaacttca tcattgttat tttcattaat ggattctggg
     9721 taagttgcgc tggtattgtt gattgaaata gatggtagca tgattttatt ccattttgat
     9781 tgatttgtta caaatatata ttacctaatg aaatagactg tcaaaaaacg ctcatttatt
     9841 tagaacctat ctttaatttg agtgtatgag cgcacaatag ccaggggggt tgagtatcct
     9901 ggaaatgcag aaaagtaaac tctttgagaa ggacgagcag aacgcctgaa aatatgcctt
     9961 tcccttcagg tttgtctgtg gataccgctt attgatttga aagggcaagc tgccaacagg
    10021 ctgtttgcaa aactcaggag gtaagcaatg aggacactac tttcggtgaa gtgggggagt
    10081 taaagtcacc tgctatgcag gtgactttat ccaggcgaac gcgttatcag aacgcgtagt
    10141 taaagttagt accgaacagc caggctttac cttcagactc gaactggtat gggccttcgt
    10201 taattttcac gctctgaccg tgcatataag aaacaccaac gtcgactgaa gcatctttat
    10261 taaatgcgta agtcgtacct gcactcagcc agaaacggtc ctggtccgga atggagatag
    10321 aacgattctg agccggaact gggctgtcat caaaggcgat accggtacgg aaggtccagt
    10381 tatcatcgta gtaataagtg gtacccaacg cgatgcggta agcatcttta aagccttcat
    10441 gtttctggaa cagcgtgtcg ccactggttg aggtcgcttt cagctgctgg aactgactcc
    10501 agctggtgta agccaggcta tagtgaatcg cccactgtgg atcaacacgg ttataacctg
    10561 acacttccca catttcaggc aggttcagcg tcagataacc cgattgcgtt gcgccacctg
    10621 tcgcggtagg aattggcaaa ccgtagttat taaacgcacg attaagatcg ctgctgtagt
    10681 tacctttgaa gtcaattttc acttcagaac ggtaggtcag tgcatagcgg ttatttttat
    10741 ccagttcata caggattccg gcgttccagc caaagcccca ctggttaccg ttcagatgag
    10801 cgattttggt attactgtcg ataccgttgg cggtagctgc caatgcttgc ccttgaggag
    10861 tttgcccggc aggagattgc ataatctgac cagcaaccaa ctgccccaga tcgcctgcga
    10921 aacgttcaat tttcgcgcga gcgtagacgg cgttgaaacc aagaccaaag ctccatgcat
    10981 tatttaagcg atacgcaccg cttaagttca ggttcatggt ttcaaggtca gttgtacccc
    11041 cgacagagcc gcctgcataa gtatcattaa actctgttgc cagaccatag ttagaggtaa
    11101 tagaagcgcc ccaaccaaat tggtcgttaa tcggtgcaac aaagtgcatg ttcggaaccc
    11161 atgccgtagg cgcgatgtta tcggctttca ggctacgacc agacggagac gttccgctga
    11221 tattcacatc cgggtcaata taaaccgcac ccgcagaaaa tgtcgggcgg tcaaacatgg
    11281 taatcaatgc ggggttacgg ctaacgttac ctgcatcatc ggcaattgcg ccttcccctg
    11341 aataagcccg gcccaggcca gaggaagaaa attcgtttaa ctgaaagcct gccgaccagg
    11401 cctgggtgga gataagtgcc actgcgactg cgagagcaga ctttgtaaac agggttttct
    11461 ggctcatgac cataacctca atgatttatt tttatacaaa ctatgttacg tgctgtaaca
    11521 ggagcgcgaa gtgtagggtc tgaggtacat cttagaaatc agaccagtgg cgagagtata
    11581 ggtcggacca gctggaatgt tgcaagtatg tttccatctt ttttcaaata tgatctaaga
    11641 aacgcgatcc agctggcaaa atttggaggt tgcgcactcg cggattcggg tgaattttgt
    11701 tttagatcat ttttaagtgt gatttcggtc acttatccga ttttgcagaa ttaacaactg
    11761 gagcagcact ttccggaagc gtaaaatatc cacaacgttt atctggcacc ttgggtgcac
    11821 atacagagga aatcaacgat gagtaaatgt agtgctgatg aaaccccggt ttgctgctgt
    11881 atggatgttg gcaccattat ggacaactcc gactgcaccg cgtcttacag ccgcgtgttt
    11941 gcaaaccgcg cagaagcaga acaaacgctg gcggcgctga ctgaaaaagc ccgtagcgtg
    12001 gaatccgaac cgtgcaaaat ctccccgact ttcactgaag agtctgacgg tgtgcgcctg
    12061 gatatcgatt tcacttttgc ctgcgaagct gaaatgctga tcttccagct gggtctgcgt
    12121 taattcctct cgggtacgtt cgcccctgtc agttgctggc aggggcgttt tcttttcttt
    12181 ctcctgtctt cactgtgtca tagctcccac tttccttttt ctccgcttgg ctaaatgtaa
    12241 aaaaatggtt aagactatga tcaggtcaga ccactttatt tattttttta caggggagtg
    12301 ttatgggtca ggttttaccg ctggttaccc gccagggcga tcgtatcgcc attgttagcg
    12361 gtttacgtac gccttttgcc cgccaggcga cggcttttca tggcattccc gcggttgatt
    12421 tagggaagat ggtggtaggc gaactgctgg cacgcagcga gatccccgct gaagtgattg
    12481 aacagctggt ctttggtcag gtcgtacaaa tgcctgaagc ccccaacatt gcgcgtgaaa
    12541 ttgttctcgg taccggaatg aatgtgcata ccgatgctta cagcgtcagc cgcgcttgcg
    12601 ctaccagttt ccaggcggtt gcaaacgtcg cagaaagcct gatggcggga actatccgag
    12661 cggggattgc cggtggggca gattcctctt ccgtattgcc aattggcgtc agtaaaaaac
    12721 tggcgcgcgt gctggttgat gtcaacaaag ctcgaaccat gagccagcga ctgaaactct
    12781 tctctcgcct gcgtttgcgc gacttaatgc ccgtaccgcc tgcggtagca gaatattcta
    12841 ctggcttgcg gatgggcgac accgccgaac aaatggcgaa aacctacggc atcacccgag
    12901 aacagcaaga tgcattagcg caccgttcgc atcagcgtgc cgctcaggcg tggtcagacg
    12961 gtaaactcaa agaagaggtg atgactgcct ttatccctcc ttataaacaa ccgcttgtcg
    13021 aagacaacaa tattcgcggt aattcctcgc ttgccgatta cgcaaagctg cgcccggcgt
    13081 ttgatcgcaa acacggaacg gtaacggcgg cgaacagtac gccgctgacc gatggcgcag
    13141 cggcggtgat cctgatgact gaatcccggg cgaaagaatt agggctggtg ccgctggggt
    13201 atctgcgcag ctacgcattt actgcgattg atgtctggca ggacatgttg ctcggtccgg
    13261 cctggtcaac accgctggcg ctggaacgtg ccggtttgac aatgagcgat ctgacattga
    13321 tcgatatgca cgaagccttt gcagctcaga cactggcgaa tattcagttg ctgggtagtg
    13381 aacgttttgc tcgtgaagta ctggggcgtg cacatgccac tggcgaagtg gacgatagca
    13441 aatttaacgt gcttggcggt tcgattgctt acgggcatcc cttcgcggcg accggcgcgc
    13501 ggatgattac ccagacattg catgaacttc gccgtcgcgg cggtggattt ggtttagtta
    13561 ccgcctgtgc tgccggtggg cttggcgcgg caatggttct ggaggcggaa taatggaaat
    13621 ggcatcagtg tttactctta atgttcgtct ggacaacatt gctatcatca ccatcgacgt
    13681 accgggtgag aaaatgaata ccctgaaggc ggagtttgcc tcgcaggtgc gcgccattat
    13741 taagcaactc cgtgaaaaca aagagttgcg aggcgtggtg tttgtctccg ctaaaccgga
    13801 caacttcatt gctggcgcag acatcaacat gatcggcaac tgcaaaacgg cgcaagaagc
    13861 ggaagcactg gcgcggcagg gccaacagtt gatggcggag attcatgctt tgcccattcc
    13921 ggttatcgcg gctattcatg gcgcttgcct gggtggtggg ctggagttgg cgctggcgtg
    13981 ccacggtcgc gtttgtactg acgatcctaa aacggtgctc ggtttgcctg aagtacaact
    14041 tggattgtta cccggttcag gcggcaccca gcgtttaccg cgtctgatag gcgtcagcac
    14101 agcattagag atgatcctca ccggaaaaca acttcgggcg aaacaggcat taaagctggg
    14161 gctggtggat gacgttgttc cgcactccat tttgctggaa gccgctgttg agctggcgaa
    14221 gaaggatcgc ccatcttccc gccctctacc tgtacgcgag cgtattctgg cggggccgtt
    14281 aggtcgtgcg ctgctgttca aaatggtcgg caagaaaaca gaacacaaaa ctcaaggcaa
    14341 ttatccggcg acagaacgca tcctggaggt tgttgaaacg ggattagcgc agggcaccag
    14401 cagcggttat gacgccgaag ctcgggcgtt tggcgaactg gcgatgacgc cacaatcgca
    14461 agctttgcgt agtatctttt ttgccagtac ggaagtgaag aaagatcccg gtagtgatgc
    14521 accgcctgcg ccattaaaca gcgtgggaat tttaggtggt ggcttgatgg gcggcggtat
    14581 tgcttatgtc actgcttgta aagcggggct tccggtcaga attaaagata tcaacccgca
    14641 gggcataaat catgcgctga agtacagttg ggatcagctg gagggcaaag ttcgccgtcg
    14701 tcatctcaaa gccagcgaac gtgacaaaca gctggcatta atctccggaa cgacggacta
    14761 tcgcggcttt gcccatcgcg atctgattat tgaagcggtg tttgaaaatc tcgaattaaa
    14821 acaacagatg gtggcggaag tggagcaaaa ttgcgccgct cataccatct ttgcttcgaa
    14881 tacgtcatct ttgccgattg gtgatatcgc cgctcacgcc acgcgacctg agcaagttat
    14941 cggcctgcat ttcttcagtc cggtggaaaa aatgccgctg gtggagatta ttcctcatgc
    15001 gggtacatca gcgcaaacca tcgccactac ggtaaaactg gcgaaaaaac agggcaaaac
    15061 gcccattgtc gtgcgtgaca aagccgggtt ttacgtcaat cgcatcttag cgccttacat
    15121 taatgaagct atccgcatgc tgaccgaagg tgaacgggta gagcacattg atgctgcgct
    15181 agtgaaattt ggttttccgg taggcccaat ccaacttttg gatgaggtag gaatcgacac
    15241 cgggactaaa attattcctg tactggaagc cgcttatgga gaacgtttta gcgcgcctgc
    15301 aaatgttgtt tcttcaattt tgaacgacga tcgcaaaggc agaaaaaatg gtcggggttt
    15361 ctatctttat ggtcagaaag ggcgtaaaag caaaaaacag gtcgatcctg ccatttaccc
    15421 gctcattggc gcacaagggc aggggcgact ctccgcaccg caggttgctg aacggtgtgt
    15481 gatgttgatg ctgaatgaag cagtacgttg tgttgatgag caggttatcc gtagcgtgcg
    15541 tgacggggat attggcgcgg tatttggcat tggttttccg ccatttctcg gtggaccgtt
    15601 ccgctatatc gattctctcg gcgcgggcga agtggttgca ataatgcaac gacttgccac
    15661 gcagtatggt tcccgtttta ccccttgcga gcgtttggtc gagatgggcg cgcgtgggga
    15721 aagtttttgg aaaacaactg caactgacct gtaataagaa ggtcaaagct atatgaatcc
    15781 gcgctgaatg gcggagtgtt ggtcaaaatg taaacgcata ttgactatac ttacgccatt
    15841 gaggtaaaaa acagcgtttc attcggtgaa tggataaggc acaatgccgg ccaccgtttt
    15901 ctttctctgg tttcagatga aagaaaacgg gcgaatctgg ttaacaaaag cggtgcaata
    15961 tgcaagtttt tatcatgcgt cacggcgacg cagccctcga tgccgccagt gattccgttc
    16021 gtcctctgac cactaatggt tgtgacgaat ctcgcctgat ggcgaactgg ctgaaaggtc
    16081 aaaaagtgga aatcgaacgc gttctggtga gcccgttcct gcgagccgag caaacactgg
    16141 aagaagtagg ggattgtctg aacctgcctt ccagtgcaga agttctgccg gagttaacgc
    16201 cctgcggcga tgttggtctg gtcagtgcct atttgcaggc gttgaccaat gaaggtgttg
    16261 cttcggtact ggtgatctct cacttaccgt tagtcgggta tctggttgcc gagttatgtc
    16321 cgggcgaaac gccgcctatg tttaccacct cggccattgc cagcgtaacg cttgatgaaa
    16381 gcggaaacgg tacattcaac tggcagatga gcccgtgtaa tctgaagatg gcaaaagcta
    16441 tctgatgtgt tcaaaattat gcccaatgag gtagattcat tgggcataat tgctcatcag
    16501 gcagcggata tctgtcaacc tcactactat cgaagactct acttatctat aaataaaaca
    16561 ctttctacat tcattcggaa attattcttc tgcgcgtatc ttgattttgc ccaaagttac
    16621 atgttgaaat gaataatgtc tgtttgacac tcattgtcat ttattaatac agataataga
    16681 gaaaatagag ttgaataatc aggcaggatg taattgaaac ttgattaaat tctggctata
    16741 gtgtttatat attatattgt caaaagtgga taaatgccaa tcgcattata tgttcattgt
    16801 ttgtttttta agctgttgat attcattatt gatatacttt ctattttttc taaatactta
    16861 ttccgtattt tttaaggatg tccttaagat aacctttctt ctgtcaatat attaattaaa
    16921 tctacattta ctctttagta aaattaaatt gcatcagggg ggtgaaaaca tcactatgcg
    16981 gccccacacc aaagaataca attttctacg acctgttctt tttggatcgc atgccgtatt
    17041 caatggctgg gtataaacat tgggtattga ctgaatcaat gtattgtaat gtttatattt
    17101 ttaattatat atagtttaag gacaaataat gagtaagttt gttaaaacag ctattgctgc
    17161 ggcaatggtg atgggcgttt tcacctctac ggcaacaatc gctgcgggta acaacggtac
    17221 tgcacgtttt tacggcacca ttgaagactc agtgtgttct atcgttccgg acgatcacaa
    17281 actggaagtt gatatgggtg atatcggtgc cgaaaaactg aaaaataacg gcaccaccac
    17341 gccgaaaagt ttccagattc gtctgcaaga ttgcgtattt gatactcagg aaacaatgac
    17401 cactaccttt actggtaccg tttcttctgc aaatagcggc aattattaca ccattttcaa
    17461 taccgatacc ggtgcggcat ttaacaatgt tagcctggcg atcggtgact ctctgggtac
    17521 ctcttacaaa agcggcatgg gtattgacca gaaaatagtg aaagacactt ctaccaataa
    17581 aggcaaagcg aagcagacac tgaactttaa agcctggctg gtcggcgcag ctgatgcgcc
    17641 agatctgggt aatttcgaag caaataccac cttccagatt acttacctgt aaggcatacc
    17701 cccatagcga cactgctatg ggggatttaa atggattttg accgaacaag aataaaaacg
    17761 gatagaaacg tgtatgcctg accattctct ttttcgatta cggattcttc cgtggtgcat
    17821 tgcgctggca atgtcaggga gttatagcag tgtctgggct gaagacgaca ttcagtttga
    17881 ttcccgtttt ctggaattaa aaggcgacac aaaaattgat ctgaagcgtt tttccagcca
    17941 gggatatgtt gagcccggaa aatacaattt acaggttcaa ctaaataaac agccattggc
    18001 ggaagagtac gatatttact ggtatgctgg tgaagatgac gcgagcaaaa gctatgcttg
    18061 tctgacaccg gaactggtag cgcagtttgg tttaaaagaa gacgtggcga acaatctgca
    18121 atggagccac gatgctaaat gcctgaaatc cggtcaactg gaaggcatgg aaattaaggc
    18181 tgatttaagc cagtccgcat tagtcatttc actgccacag gcttacctcg aatatactta
    18241 tcccgactgg gatccgcctt cacgttggga tgacggcatc tccgggatcg tcgcggacta
    18301 cagcatcaac gcacaaaccc ggcatgaaga aaatggcggt gatgatagta acgagatcag
    18361 cggcaacggg acggtcgggg ttaacctggg gccgtggcgt atgcgtgctg actggcagac
    18421 taactatcaa catactcgca gtaatgatga tgacgatgaa ttcagcggcg atgaaactca
    18481 aaaaaaatgg gagtggagtc gctactatgc ctggcgggcg ttaccatcat taaaagccaa
    18541 actggcgctg ggcgaggatt acctcagatc cgatattttt gatggtttta actatgttgg
    18601 tggcagtgtc agtactgacg atcaaatgtt gcctcccaat ctgcgcggct acgcgccaga
    18661 catttccggc gtggcacaca ccacagcaaa agtgaccgtc agccagatgg ggcgtgtgat
    18721 ttacgaaacg caggttccgg ctggaccgtt tcgtattcag gatcttggtg attctgtctc
    18781 cggtacgttg catattcgca ttgaagaaca gaacggccag gtgcaggaat atgacatcag
    18841 caccgcctcg atgccatacc tcactcgccc aggtcaggtt cgctataaga tcatgatggg
    18901 ccgtccgcaa gagtggggac accatgtcga gggtgaattt ttttctggtg ctgaagcttc
    18961 ctgggggatc gctaacggct ggtcgttata tggcggcgca ctgggagatg aaaactatca
    19021 gtctgcggcg cttggcgtcg gtcgcgattt gtctacattc ggcgcggtcg cgtttgatgt
    19081 tactcactcg cacaccaaac tggataaaga caccgcttat ggcaaaggtt cgctggacgg
    19141 taactccttc cgtgtgagtt attccaaaga ctttgaccag ctcaacagcc gcgttacttt
    19201 tgctggatat cgcttctcgg aagagaactt tatgaccatg agcgagtatc tggatgccag
    19261 tgacagcgga atggtacgca cgggcaacga caaagagatg tacaccgcca cttataacca
    19321 gaacttccgc gatgcgggtg tttcggttta tctcaactat acccgccata cctactggga
    19381 tcgcgaggag cagacaaact acaacatcat gctctcgcac tatttcaata tgggcagtat
    19441 tcgtaatgtc agcatctcga tgactggcta ccgttacgag tatgacaacc aggccgacaa
    19501 aggcatgtac atttcgctca gtatgccgtg gggcgacaac agtaccgtta gctataacgg
    19561 taactatggc agtgggacgg acagcagtca ggtcggttat ttcagccgtg tcgatgacgc
    19621 gactcactat cagttgaacg tcggcaccag tgacaaacac accagcgttg acggctatta
    19681 cagccatgat ggttcgctgg cgcaggttga cctcagtgcg aactaccatg aagggcaata
    19741 cacctctgcg ggcttgtcgt tacagggcgg cgcaacgctt actgcccacg gtggcgcact
    19801 tcaccgtacc cagaatatgg gcgggacacg cttgttgatt gatgccgatg gcgttgccga
    19861 tgttccggtg gaaggtaacg gggctgctgt ttataccaat atgtttggta aagccgtcgt
    19921 ttctgacgtc aataactatt accgcaatca ggcgtatatc gacctcaaca gattgcctga
    19981 aaacgctgaa gcaacccagt cggtggtgca agccacgcta actgaaggtg ccattggcta
    20041 ccgtaaattt gctgtcatta gtggacaaaa agcgatggcg gtgctgcgcc tgagcgacgg
    20101 cagccatcct ccgtttggcg cagaagtaaa aaatgataac gagcagacag tgggccttgt
    20161 cgatgatgac ggcaatgttt atctggctgg ggtgaaacct ggcgaacaca tgagtgtgtt
    20221 ctggagtggt gttgcgcatt gcgatatcaa cctgccggac ccgctgcctg ccgatctgtt
    20281 taacggcttg ttactgccat gccagcataa aggcaatgta gcacctatca cttcgccggc
    20341 ggtcaaaccg gcgattcagg aacagacaca gcgggtgacg ccaacggaac ctccgacttc
    20401 aatttcagta aaccagtaac gtgattaagg aatgatccat gtttaatctg actaacactg
    20461 caaaaatcgt tgtcccggca ctggcgctgc tggcgacagc ggtcagtttc tccagccacg
    20521 cttcggtgac gcccgacagg acacggctgg tgtttaacga aagcgacaag tccatcagcg
    20581 tcacgctgcg caacaacgat cccacactgc cttatctggc gcagagctgg atagaggatg
    20641 caaagggcaa caaaatcagc tcgccgcttg cggtgcttcc gccagtgcag cgcatcgatg
    20701 cgatgatgaa cggccaggtg aaggtgcagg ggctgccgga tatcaacaaa ctgccggccg
    20761 accgcgagag cgtgttctat ttcaacgtgc gcgaaattcc gccgaaatca aataaaccga
    20821 acacactgca gattgcgctg cagacgcgta tcaaactgtt ctggcgaccg aaggcgctgg
    20881 agaacaccag catgaaaagt ccgtggcagc ataaggtgac gctcatccgt aacggccagg
    20941 acttcaccgt caataacccg acgccgtact acgtgatcat cagcaacgcc agcgctaaaa
    21001 aaggcggcaa cccgccggcg ggattcacac cgctgatgat ggagccgaaa acctccgcac
    21061 cgctgaatgc gaagatggac agcgtgccgg tgctgactta catcaacgac tacggcgcgc
    21121 ggatgccgct gtacttcata tgcaccggca acagctgcca ggtggatgaa gagcagagcc
    21181 gcaaaggctg atgccgggag ggacttatga gtcacaagtt tgtagcgggc gcagggttgc
    21241 tggtgctggc cggatggggc atcagcggtt tggctgttgc tggcagtgat atcaaacagg
    21301 tggatctgac gctgcgaatc ctggtcgatg gaccgccgcc ttgctccgtg aagggaagcg
    21361 ctgtgaaatt tggcaatgtg ataatcaaca ggatcgatgg taccaattac caacaggatg
    21421 cgaaatacac cctcaactgc ggtaataaag tcagcgatga cttacggatg cagctcaaag
    21481 gcaataccgc cgtgatcaac ggagaaacgg ttctcagcac ggatatcgcc ggcctggggg
    21541 tgcggataga aaacgccgcc gacaactccc tgtttgcagt tggtgaaaat aactggacgc
    21601 cttttaatgt caacactcag cctcagctga aagcggtgcc ggtaaaacag agcggggtgc
    21661 aactgacgcc gtcggagttt aacgccacga tgaccatggt ggtggattac caatgaaaaa
    21721 attcctgctc cttttactgt gcgctggttg cggggctgtg ctggcggcgg aagatgacat
    21781 cacattccac ggtacgctga tgtcgccgcc cgcctgtagc atcagcggcg ggcaaaccat
    21841 cgaagtgcaa tttcgcgatc tcattatcga cagcattgat ggcaattatg gtcgtcagaa
    21901 agtggactac gaactgagct gcgactcgga tgtccgcgat cccgagtgga atatgacact
    21961 ctcctggacg gggactgcca cctcctacaa cgactctgct attgagacgg atgtaccggg
    22021 cttcggaatt gagcttcagc atgacggtca gcgttttaag ctcaacgagc cgctttccat
    22081 taacgccacc gattttagcc agaagccaaa gctggaggcg gtaccggtca aagcggctga
    22141 tgcggtgctg acggacacga ccttttctgc ttacgcaacc ttacgggtgg attaccaatg
    22201 aaaacagcac acatttattc acccggttat gcgctgttag caggcctgct gctgaccgcc
    22261 agcacaggag ctcaggcagt cgacaacaac ctgcattttt acggcaacct gctgagtaaa
    22321 tcctgcacgc tggtggtgga tggtgccaac ctggcagaaa tccattttcc caccgtcagc
    22381 aaccgggatt tgatggtatc tgggcagtcc gcgcatctgc cggttgtgtt taagctgaaa
    22441 gactgcaaag ggccggcggg atacaacgtc aaagtcacgc tgagcggcac cgaagacagc
    22501 ggtcagccgg gttttctggc agtcgatgac acctccaccg cgcagggcgt ggggataggg
    22561 atggaaacgc tcgacggcaa gcgggtggat attaacaaca ccaccggggc gacctttgcg
    22621 cttaccaacg gcaacaatga tattaatttc cgggcctgga tccaggcgaa aagcggtcgt
    22681 gatgtaacca tcggtgattt caccgccagc ctgacagcaa cttttgagta tatttaaaat
    22741 gaaaatcctt cgtcatgttt tatgtctttt attgctgtta atgttcaata catgcggcca
    22801 ggcggcgacg aaagttggtg agttatatgt gccagctggt gccagttcac ctgcgacaga
    22861 gtttgagttt catctgatta tagcgacgcc agattcagtt aattacgcta tatttaaggc
    22921 tacattcatg cccaattcac agccggattt agtttcgtgg actggcgata gttctactca
    22981 accatctatg tcgaaaattt cggacagcag ggtatccatg tcagcctgtc ctggattaga
    23041 gcagtatgat agccagacca aaacgggatg gacatgtaat gaattaaaga tgtttgttta
    23101 ttacgatggg aatttacatg gttgtccgtg gattgtgtcg tcgtttgtta agagtagaga
    23161 tcccttcgct aaaacgtatg atgatgattt tcctgactat attggcccaa cgaaggtgag
    23221 ttcttcatgt ccggcggtgc ccctggcacc gtatgatgtt tcctggaatg aaaattatgt
    23281 tgtacataac aaagtcgtca ggttgcagtc aacaggtggt gttattgagc aaacactgcc
    23341 cactttcctg atggaaaatg gcaaactgtg taacgggaat aattttgatg agcgtggtgc
    23401 ctactgccgt tttatcgcac agcagatgac cttctcgact tccggctgtg ataatgccaa
    23461 agtgacggtg acgcctgagc cgcaaccgat tacgtccagg caactgcatg atatgaagct
    23521 acgcgtggac acgacatctc gacagcccat agattctacc tgccgattca cttacatact
    23581 gaatatgtat tagcgcacct tttctcacct ggcagggtca ttcgcccctg ccattccgcg
    23641 ctcctggcac aacacgctca actcctcagg gcaactccgg cggcaaccac tcttccactt
    23701 caatcaacac caacaaagcc gcatcaccgc cgtactcttt cggtgcctgg tgaaatgcca
    23761 tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg cttcaaaata tgcttcccgt
    23821 gcccatgcat cacgcaggca caaaacacat gttcgcggcg acaggcggca atcaacgcgc
    23881 ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa atccaaaaac aattccggtg
    23941 aataatcgcc gcggcgcagt ttcttcgcct caaaatggct gacatccggg cgaacatatt
    24001 tcaccggacc ttcggtattt aataacggct gaaactcatc ggagaaataa tggctggcat
    24061 cagcctgctc ctggatcaag cgtttcaccg gcacttcgct gattttttta cgctgcggtc
    24121 ggtggacaat cgtgtcctgc ttaatcttgc gactccccgc catcaactgg cgaaacagag
    24181 cctggtcctc ctcgctaagt gttgttttct ttttcattta tctgtctcta tttctaattt
    24241 tcctacagtt tacccgactc gtagcacaat cgatcgtttt aaacgtattt ttcctcactc
    24301 accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa actagccgcc gaaattattg
    24361 cgatcatgcc ctggaggaat acgtggataa aattttcgtt gatgaagcag taaatgagct
    24421 gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc ttcagcgcgg caaatatctg
    24481 gtacggtcac ggcaccgata acccgtggga tgaagccgta cagctggtgt taccatcgct
    24541 ctacctgccg ctggatatcc cggaagatat gcgcaccgcg cgtctgacct ccagcgaaaa
    24601 acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag cgcattccgg tggcttacct
    24661 gaccaacaaa gcctggttct gcggtcatga attttacgtc gatgaacgcg tgctggtgcc
    24721 gcgctcaccg attggtgaac tgatcaacaa taaatttgcc ggactcatca gcaagcaacc
    24781 gcagcatatt ttagatatgt gtaccggtag cggctgcatc gccattgcct gtgcttatgc
    24841 cttcccggaa gcggaagtcg acgcggtaga tatctctccc gacgcgctgg cggttgccga
    24901 acagaacatc gaagaacacg gtctgatcca caacgtcatt ccgattcgtt ccgatctgtt
    24961 ccgtgatttg ccgaaagtgc aatacgatct gattgtcact aacccgccgt atgtcgacgc
    25021 ggaagatatg tccgacctgc caaacgaata ccgtcacgag ccggaactgg gcctggcatc
    25081 tggcaccgac ggcctgaaac tgacgcgtcg cattctcggt aacgcggcag attaccttgc
    25141 ggatgatggc gtgttgattt gtgaagtggg caacagcatg gtacatctta tggaacaata
    25201 tcccgatgtt ccgttcacct ggctggagtt tgataacggc ggcgatggag tgtttatgct
    25261 caccaaagag cagcttattg ccgcacgaga acatttcgcg atttataaag attaagtaaa
    25321 cacgcaaaca caacaataac ggagccgtga tggctggaaa cacaattgga caactctttc
    25381 gcgtaaccac cttcggcgaa tcgcacgggc tggcgctcgg ctgcatcgtc gatggtgttc
    25441 cgccaggcat tccgctgacg gaagcggacc tgcaacatga ccttgatcgt cgtcgccctg
    25501 ggacatcgcg ctataccacc cagcgccgcg agccggatca ggtcaaaata ctctccggtg
    25561 tttttgaagg cgttactact ggcaccagca ttggcttgtt gatcgaaaat accgaccagc
    25621 gttctcagga ttacagcgca attaaagacg tcttccgtcc aggccatgct gattacacct
    25681 acgaacaaaa atacggtctg cgcgattatc gcggcggtgg acgttcttcc gcccgcgaaa
    25741 ccgccatgcg cgtggcggca ggggcgattg ccaaaaaata tctcgccgag aaatttggta
    25801 ttgaaatccg cggctgcttg acccagatgg gcgacattcc gctggaaatc aaagactggt
    25861 cgctggttga acaaaatccg ttcttctgcc cggacccgga caaaatcgac gcgttagatg
    25921 aactgatgcg cgcgctgaaa aaagagggcg actccatcgg cgcgaaagtc accgttgttg
    25981 ccagtggtgt ccccgccgga cttggcgagc cggtctttga ccgcctggat gccgacatcg
    26041 cccatgcgct gatgagcatc aacgcggtga aaggcgtgga aattggcgac ggttttgacg
    26101 tagtggcgct gcgcggcagc cagaaccgcg acgaaatcac caaagacggt ttccagagca
    26161 accatgcggg cggcattctc ggcggtatca gcagcgggca gcaaatcatt gcccatatgg
    26221 cgctgaaacc gacctccagc attaccgtgc cggggcgcac tattaaccgc tttggcgaag
    26281 aagttgagat gatcaccaaa ggccgtcacg atccctgtgt cgggatccgc gcggtgccga
    26341 tcgctgaagc gatgctggcg atcgttttaa tggatcacct gctacggcag cgggcgcaaa
    26401 atgccgatgt gaagactgat attccacgct ggtaaaaaat gaacaaaacc gcgattgcgc
    26461 tgctggctct gcttgccagt agcgctagcc tggcagcgac gccgtggcaa aaaataaccc
    26521 aacctgtgcc gggtagcgca caatcgatag gcagtttttc taatggctgt attgtcggcg
    26581 ctgacacgtt gccgatacag tccgaacatt atcaggtcat gcgtaccgat ctgcgtcgct
    26641 atttcggtca cccggatctg gtgatgttta tccagcgtct gagtagccag gtgagcaatc
    26701 tgggcatggg cacggtgctg attggcgata tggggatgcc cgctggtggg cgtttcaacg
    26761 gcggtcatgc cagccaccag accggactgg atgtcgatat ttttctgcaa ctgccgaaaa
    26821 cacgctggac ctccgcgcaa cttttgcgcc cgcaagcact ggacttagtt tcccgcgacg
    26881 gtaaacacgt tgttccgacg ctgtggaagc cagaaatttt cagcttgatc aaactcgccg
    26941 cccaggacaa agacgtcaca cgcatttttg ttaatccggc gattaaacaa caactttgcc
    27001 ttgatgcggg caccgatcgc gactggttgc gcaaagtgcg accctggttc cagcatcgcg
    27061 cgcatatgca tgtacgatta cgttgccctg ccgatagtct ggagtgtgaa gatcaacctt
    27121 taccgccacc aggcgatggt tgcggggcag aactgcaaag ctggtttgaa cctccaaaac
    27181 cgggaacaac aaagcctgag aagaagacac cgcctccgtt gccgccttcc tgccaggcgc
    27241 tactggatga gcacgtgatc taatggaaac gtttaatagc ctgtttatgg tttccccgct
    27301 gttgctggga gttctctttt ttgtcgccat gctggcggga tttatcgact cgattgccgg
    27361 tggcggtggg ttactcacta ttccggcatt gatggctgcg ggaatgtctc ccgctaacgc
    27421 gctggcaacc aataaactgc aagcctgcgg cggctctatt tccgccacta tctactttat
    27481 tcgccgcaaa gtggttagtt taagcgatca gaaactcaat atcgccatga cctttgtcgg
    27541 ctcaatgagc ggcgcactgc tggtgcaata cgttcaggct gatgtcttac ggcagatttt
    27601 gcccattctg gtaatttgta tcggcctcta tttcttgctg atgccaaagc tgggtgaaga
    27661 agatcgccag cgtcggatgt acggtttacc cttcgcgtta attgccggtg gctgcgtcgg
    27721 tttttatgac ggattctttg gcccggctgc cggatcgttt tacgcgctgg ccttcgttac
    27781 gctgtgcgga ttcaacctcg ccaaagccac ggctcacgcc aaattactca acgcaacgtc
    27841 aaacattggc ggtctgctgc tgtttattct cggcggcaaa gtgatttggg cgacgggctt
    27901 tgtgatgctg gtggggcagt tcctcggcgc gcgcatgggt tcacggctgg tgttgagcaa
    27961 aggacaaaag ctgatccgcc cgatgattgt tatcgtctcg gcggtgatga gtgccaaatt
    28021 actttacgat agccacggac aggagatcct ccactggttg gggatgaact aatgaacagt
    28081 acacaccact atgagcagtt gattgaaatt tttaatagct gctttgccga tgaatttaat
    28141 acccgtctga ttaaaggcga cgacgaaccg atctatcttc ctgctgatgc ggaagtaccg
    28201 tataaccgaa tcgtctttgc tcatggcttt tatgccagcg caattcatga gatttcgcac
    28261 tggtgtattg ccgggaaagc gcgccgtgag ctggttgatt tcggctactg gtattgcccg
    28321 gatgggcgtg atgcccaaac gcaaagccag tttgaagatg ttgaagtgaa gccgcaggca
    28381 ctcgactggt tgttctgtgt ggcggcggga tatccgttta acgtcagttg cgacaatctg
    28441 gaaggcgact tcgaaccgga tcgtgtggtg tttcaacggc gggtacatgc gcaggtgatg
    28501 gattacctag caaacggcat tcctgaacgc ccggcacgct tcattaaagc actacaaaat
    28561 tattatcaca cgccggaact tacggcggaa cagttcccgt ggccggaagc gctcaactaa
    28621 agccagcgcc acgttttaca atgaggaaag aagatgatcg cggagtttga atcacgcatt
    28681 ctggcattaa tcgacggtat ggttgaccat gccagtgatg atgagttgtt tgccagtggg
    28741 tatttgcgtg gccacctgac attagccatc gcagaactgg aaagtggtga tgaccactcc
    28801 gctcaggcgg tgcatacgac cgttagccag agtctggaaa aagccattgg tgccggtgaa
    28861 ttgtcgccgc gtgaccaggc gctggtgacc gatatgtggg aaaacctgtt tcagcaggcg
    28921 tcacagcagt aagttgcggc gtctggagca ccatgcctga tgcgacgctt atcgcgtctt
    28981 atcaggccta cacgtcacac gtaggtcgga taaggcgttc acgccgcatc cggcaaacac
    29041 tgcgagagca gattaccccg ccttaaccgc tttacccttc aacaatttcc gcacccataa
    29101 ccgattcggg tttaacgccg ccagcgtact ggcatccatc ggaatcggtt cgtcgctcat
    29161 ctgcgccgcc agaatctcgg cacacagcgg ggcagaacac aaaccgcgag aacctaaagc
    29221 cgcaaacata aagagatcgt caaaaaccgg cgcgcttacc gcctcatctt tctgctccgc
    29281 caacgacgca tattccacga gtgttgcctc ataatcggga acattgccta ccattggcag
    29341 atgatcgcgg gtggcacaac gcacaccgca gcgcgcctct ttatcactga catcaacctc
    29401 ttttgcccac tgtgcctgcg ggaaacaatc aatcaaccgc tggcgattct gctgctgatc
    29461 gtcctcactg tacgccgtat cttcgctgcc gcgatgataa ctggcaccaa tacaatgatg
    29521 ttgattcgcc ggattttgtg gcgtgagata accgtcatag cacagcacct gcttcagctc
    29581 tgccaattcc ggcgttgtcg gaatatggct gacctgcccg gcaaccgaat acaccgggag
    29641 agtcgacgta tggctgaatc ggctgatttg atgcccgttc gccagtacca ctacgctgtg
    29701 tgttgcttgc tgatctcctg caaaattcaa caaccaacag tcatccttac gggataaatt
    29761 ctgtaactga tattgataat aaatctgcaa accctgctgt tgcgccagtt ccagcacatt
    29821 acgggtcagt tctgctgggc acagccaacc accttgcgga taagtaatgc cgctgcaatt
    29881 tgtcgcaacg cccgtaattt gttcaaccgc atttgcctca acggctacag ccagttctgc
    29941 gggtaaatcc attgacaaca tctgtgcgat tttatgctgg cttttctcat tccagcctaa
    30001 ctgcgtgacg ccgcaccagt catgatcaaa tttaacgggt aattggtcgt aaaaccgacg
    30061 agcaaaagta aacgcattag agaaaaagcg gtttagcgcc tcatcgtgtt tgcttaataa
    30121 cggatacagc gccccctggc gattgccgga agcacccagt gcgggggcct catccgcgca
    30181 ataaagcgtt acctgccagc cgcgccgtaa tagcgccagc gacaacaacg cgctggcaat
    30241 accaccgccg ataatcgccg cttcccgttt gctgctgccc gtgcggttaa accacggcgc
    30301 ggagcagggg agcggtaatg tctgttccat caccccgcaa agcatttccc gtttgcgccc
    30361 aaagccctta cgtttttgca tcgtgaatcc ggcgtcctgc aaaccgcggc ggacaaaacc
    30421 ggcagacgta aatgtcgcta gcgtgccgcc cggacgcgcc aaccttgcca tggcgttaaa
    30481 cagattttgc gtccacatat ccgggttttt cgctggcgca aagccgtcca gaaaccaggc
    30541 atctactttt tgatttagcg aatcgtccag ttggctggtc agttcgttaa tatcgccaaa
    30601 ccataaatcc agcgtcacgc ggccttcatc gagcaataaa cgatggcaac cgggcaaggg
    30661 cattggccac tgcgcctgaa gttgttctgc ccacggagcc agttccggcc agtgttgatg
    30721 cgctaaggct aaatccgcac gggtgagggg aaatttctca aaactaatga aatgtaagcg
    30781 ttgtaattgc gcttgcggat gcgcttcgcg aaactgatca aatgcctgcc atagcgtcag
    30841 gaagtttaat ccggtgccga agccgctctc tgctaccaca aacagaggat gtggatgctc
    30901 aggaaagcgt acctctaatt ggttgcctcc cagaaaaaca taacgcgtct cttccagccc
    30961 gttatcgttg gaaaaataga catcgtcaaa atctcgggaa acaggtgtac cctcagcatt
    31021 aaattcgagg ttggcaggtt gtatggagta gtgtttcacg taagttactc gtcttacagg
    31081 cggtggctcg atcttagcga tgtgtgtaag gctgcgcaaa tttctctatt aaatggctga
    31141 tcggacttgt tcggcgtaca agtgtacgct attgtgcatt cgaaacttac tctatgtgcg
    31201 acttacagag gtattgaatg aaacgtgcag tgattactgg cctgggcatt gtttccagca
    31261 tcggtaataa ccagcaggaa gtcctggcat ctctgcgtga aggacgttca gggatcactt
    31321 tctctcagga gctgaaggat tccggcatgc gtagccacgt ctggggcaac gtaaaactgg
    31381 ataccactgg cctcattgac cgcaaagttg tgcgctttat gagcgacgca tccatttatg
    31441 cattcctttc tatggagcag gcaatcgcag atgcgggcct ttctccggaa gcttaccaga
    31501 ataacccgcg cgttggcctg attgcaggtt ctggcggcgg ttccccgcgt ttccaggtgt
    31561 tcggcgctga cgcaatgcgc ggcccgcgcg gcctgaaagc ggttggcccg tatgtggtca
    31621 ccaaagcgat ggcatccggc gtttctgcct gcctcgccac gccgtttaaa attcatggcg
    31681 ttaactactc catcagctcc gcgtgtgcga cttccgcaca ctgtatcggt aacgcagtag
    31741 agcagatcca actgggcaaa caggacatcg tgtttgctgg cggcggcgaa gagctgtgct
    31801 gggaaatggc ctgcgaattc gacgcgatgg gtgcgctgtc tactaaatac aacgacaccc
    31861 cggaaaaagc ctcccgtact tacgacgctc accgtgacgg tttcgttatc gctggcggcg
    31921 gcggtatggt agtggttgaa gagctggaac acgcgctggc gcgtggtgct cacatctatg
    31981 ctgaaatcgt tggctacggc gcaacctctg atggtgcaga catggttgct ccgtctggcg
    32041 aaggcgcagt acgctgcatg aagatggcga tgcacggcgt tgatacccca atcgattacc
    32101 tgaactctca tggtacttcg actccggttg gcgacgtgaa agaactggca gctatccgtg
    32161 aagtgttcgg cgataagagc ccggcgattt ctgcaaccaa agccatgacc ggtcactctc
    32221 tgggcgctgc tggcgtacag gaagctatct actctctgct gatgctggaa cacggcttta
    32281 tcgccccgag catcaacatt gaagagctgg acgagcaggc tgcaggtctg aacatcgtga
    32341 ccgaaacgac cgatcgcgaa ctgaccaccg ttatgtctaa cagcttcggc ttcggcggca
    32401 ccaacgccac gctggtaatg cgcaagctga aagattaatt cgcagtaggc cggagtagtc
    32461 ggagtagtcg gagtagtcgg agtagtcgga gtaggtcgga taagacgcgc cagcgtcgca
    32521 tcctacgtta cgcgctaatg cggcctccgg cactaacgca aaagggaacc tgatggttcc
    32581 ctttttcaca tcattgacaa tcgccgcctg ttccaggcaa acttcccgct ttgtcgattt
    32641 ccttctgaaa agacgtacgc gttaaatcct gccaacgcac tgtaaccctg aaaccagaga
    32701 gatgagacgg ggatactcct cgccttgcgc tgcattctgg agtaatgcat gactgctgta
    32761 agccaaaccg aaacacgatc ttctgccaat ttttcgctct tccgcatcgc ttttgcggtt
    32821 tttctcacct acatgaccgt agggctgccg ttgccggtta tcccgctgtt tgttcatcat
    32881 gatctgggct atggcaatac catggtcggg attgtcgtcg ggattcagtt tctggctacg
    32941 gtgctgacgc gtggctatgc cgggcgactg gccgatcaat atggcgcaaa acgttcggcg
    33001 cttcagggga tgttagcttg tggtctggct ggcggcgcgt tgctgctggc ggcgattttg
    33061 cctgtctccg caccgttcaa atttgccctg ttggtcatcg ggcgtttgat tcttgggttt
    33121 ggtgaaagcc agttactgac aggcgctctg acctgggggt taggcatcgt aggggcaaaa
    33181 cactctggca aagtgatgtc atggaatgga atggcgattt acggtgccct cgctgttggt
    33241 gctccgcttg gcctgttgat tcatagccat tacggttttg ccgcactggc gatcaccaca
    33301 atggtattac ccttactggc gtgggcctgt aacggcacag tgcgcaaagt accggccctg
    33361 gcgggagaac gtccatcgct gtggagcgtt gtcgggttta tctggaaacc agggttaggg
    33421 ctggcactac aaggcgttgg ttttgcggtt atcgggactt tcgtttcgct ctactttgcc
    33481 agcaaaggat gggcgatggc gggctttact cttaccgcgt ttggcggcgc atttgtcgtg
    33541 atgcgcgtca tgtttggctg gatgccggac cgttttggcg gcgtgaaagt ggcgattgtc
    33601 tctctgcttg tagaaacggt gggcttgttg ttgctctggc aagccccagg tgcgtgggtc
    33661 gcattagcgg gcgcggcgtt aaccggagcc ggatgttcgc ttatctttcc tgcgctgggc
    33721 gtggaggtgg ttaaacgcgt cccctcacaa gttcgcggca ccgcactggg cggttacgcc
    33781 gcgtttcagg atatcgccct cggcgtctcc gggccgctgg cgggaatgct ggcgaccacg
    33841 tttggttact cttcggtatt tcttgccggg gcgatctctg cggtgctggg aattattgtc
    33901 acgatactgt catttcgtcg gggctaacga accagccaga ccagcatcag gataatcgcg
    33961 actaacagta tccacagcgc gggacgcgtt gccatatttt gcaacgtgtc catcatcggt
    34021 ggaaaaggat taagcagcgg ttgcatatca cgcggatcaa tccctttaac ccgccgttga
    34081 taaagctgat taagaatatc ctcggcctgg gcagaagaaa gcgatgattg cgcagaaagg
    34141 ccatattttt gctggatata tgccgataac gccgccagtt cactggcatc taaaggttgt
    34201 tttagcgcca tctgtagtga ttccagcgtc ggcgtatttt gctggcttaa cgtctggcgc
    34261 gcctgcaacc aggtcaccag atggttaaac agtttcgctg gaattaactc gccatctttc
    34321 accccggaaa gttccagcat cgattgccag atctgtttgc tgggttcccc cgttgccgcc
    34381 gcaagtttgg tcaccagctg ttttagcgca ttgtgctccg ccggtaataa aggacggtcg
    34441 gtcgcctcgc gctgctgtgg ttgcggaata accatcttcc cttcctgcaa cagggtgaga
    34501 atggttttta attgctccgg tgagagctga ttcagcggcg tctgaccaaa gttatgacgg
    34561 atataatccg tgaccgcctg acgattattc cccagacgta aatactcccc taattgcgct
    34621 aaaagctggc gggcagaatg gcttttttgc gcggccagca gacgttgcgc cagattatgc
    34681 tcagcggcag ggaagtgacg cgaaagcagc ggtgaatctc ccgacagacc aatatcgtgc
    34741 ctgatacccg cccacagttc tgctctttgt tgttgcgtca gtgaggtcac tttcgtcatt
    34801 aagctttcca gcgaagtacg ttgctgactg gataaaggct gattgcccgc gccagacggc
    34861 aggttatctc cctgacctgg tggttgccca ggaggagggc cggaaatagg ttgtatcatt
    34921 acgtatcctt atacctgaaa tcttcgcaag tatgcctggc cgcgagatta tggcacactt
    34981 gtccggttaa ctctcgtctc atacaggtaa cacaaacgtg aaaatccttg ttgatgaaaa
    35041 tatgccttat gcccgcgact tatttagccg tttgggtgag gtgaccgcgg ttcccgggcg
    35101 tccaatcccc gttgctcaac tggcagacgc ggatgcgctg atggtgcgtt cggtcacgaa
    35161 agtgaatgaa tctttgctgg caggaaaacc cattaaattt gttggcactg ccactgcggg
    35221 gaccgaccat gtcgatgaag catggttgaa gcaggcggga attggttttt ccgctgcacc
    35281 tggctgtaat gcgattgcgg tggtggaata tgttttctcc tcactactga tgcttgccga
    35341 acgcgatgga ttttcactgc acgaccgtac ggtggggatc gtgggcgttg gtaacgttgg
    35401 acgtcgattg caggcgcgac tggaagcgtt agggattaaa acattgcttt gcgatccgcc
    35461 tcgcgctgac cgcggggatg agggtgattt ccgctcgctg gatgaattag ttcagcatgc
    35521 ggatattctg actttccata cgccactctt caaagatggg ccgtacaaaa cgctgcatct
    35581 ggcggatgaa aaactgatcc gtagcctgaa gccgggagcg attctgatta acgcctgccg
    35641 tggcgcagtc gtcgataata ctgcgttgct gacctgcctg aatgaaggcc agaagttaag
    35701 cgtagtgctg gatgtctggg aaggcgaacc ggaacttaac gtggaattgc tgacaaaagt
    35761 ggatatcggc acgccgcata tcgcaggcta taccctggaa ggtaaagcac gcggtactac
    35821 gcaagtgttt gaagcctata gcaagtttat tgggcatgaa cagcacgttg cgctggatac
    35881 attactgccc gcgccagagt ttggtcgcat tacgctgcat ggcccgctcg atcaaccgac
    35941 gctgaaaagg ctggtgcatt tggtgtatga tgtgcgccgc gatgacgcac cgctgcgtaa
    36001 agtcgccggg ataccgggtg agttcgataa actgcgcaaa aactatcttg agcgccgtga
    36061 atggtcatct ctgtatgtaa tttgtgatga cgccagtgcg gcatcattgc tgtgtaaact
    36121 gggttttaac gccgttcatc atccggcacg ttaatctctt cttcatgctc tctgctgtaa
    36181 cattggcagg gagctttgct atttctggag taaaccacca tgtctgaagg ctggaacatt
    36241 gccgtcctgg gcgcaactgg cgctgtgggc gaagccctgc ttgaaacgct ggctgaacgt
    36301 cagttcccgg ttggggaaat ttatgcactg gcacgtaacg aaagcgcagg cgaacaactg
    36361 cgctttggtg gtaagacaat caccgtgcag gatgccgctg aattcgactg gacgcaggcg
    36421 cagctggcat tttttgtcgc aggcaaagaa gctaccgctg cctgggttga agaagcgacc
    36481 aactcaggtt gcctggtgat cgacagcagc ggactgtttg ctctcgaacc cgacgtaccg
    36541 ctggtggtgc cggaagtaaa cccgtttgta ctgaccgatt accgcaaccg gaatgtcatc
    36601 gccgtaccag acagtctgac cagccagctg ctggcggcac tgaaaccgtt aatcgatcag
    36661 ggcggtttgt cgcgtatcag tgttaccagc ctgatttcag cctccgccca gggcaaaaaa
    36721 gcggtcgatg cgttagcggg gcagagtgcg aaattgctca acggcattcc gattgacgaa
    36781 gaagatttct tcggtcgtca actggcgttc aatatgctgc cgttgttgcc ggatagcgaa
    36841 ggtagcgtgc gtgaagaacg tcgtatcgtt gacgaagtac gcaaaatcct gcaggacgaa
    36901 gggctgatga tttcggcaag cgtcgtccag gcaccggtat tctacggtca tgcccagatg
    36961 gtcaactttg aagcactgcg tccgctggcg gcagaagaag cgtgtgatgc gtttgctcag
    37021 ggcgaagata ttgtgctctc tgaagagaac gaattcccga ctcaggtagg ggatgcttcg
    37081 ggtacgccgc atctttctgt tggctgcgtg cgtaatgact acggtatgcc ggagcaagtc
    37141 cagttctggt cggtggccga taacgttcgc tttggcggcg cgctgatggc agtaaaaatc
    37201 gccgagaaac tggtgcagga gtatctgtac taatgtccga ccagcaacaa ccgccagttt
    37261 ataaaattgc gctgggcatt gagtacgacg gcagtaagta ttacggctgg caacggcaga
    37321 atgaagtccg cagtgtgcag gagaagctgg aaaaggcgct ctcccaggtg gcgaacgaac
    37381 ccattaccgt cttctgcgcc gggcgtactg acgctggtgt acacggtacc gggcaggtag
    37441 tgcatttcga aacgaccgcg cagcgcaaag acgcggcgtg gaccttgggc gtaaatgcga
    37501 atttacctgg tgacatcgcc gtgcgttggg ttaaagctgt acctgatgat tttcatgccc
    37561 gatttagcgc cacggctcgc cgttatcgct acatcatcta caatcatcgg ctgcgcccgg
    37621 cggtactgag taaaggggta acccattttt acgaaccgct ggacgctgaa cggatgcatc
    37681 gggctgcgca atgcttgctg ggcgagaatg atttcacctc gttccgtgcg gtgcagtgcc
    37741 agtcccgaac cccgtggcgt aacgttatgc atattaatgt cacgcgtcac ggtccttatg
    37801 tggtggtaga tatcaaagcg aatgcctttg tacatcatat ggtcaggaat attgtcggca
    37861 gcctgatgga agtcggtgcc cacaaccagc cggagagctg gatagcagag ttgctggcgg
    37921 caaaggacag aacgctggcg gcagcaacgg caaaagcgga agggctgtat ctggtcgcgg
    37981 tggattaccc tgaccggtat gatcttccaa aaccgccaat gggcccgcta tttctggcgg
    38041 actaacgaga ataatgctcg gaacatttca ggctgtagcc aacgtagtta cagcctgaaa
    38101 gatgacgagt acaaggcata ggcaaatatg gacctgattt atttcctcat cgattttatc
    38161 ctgcacattg acgtgcatct ggcagagctg gtcgcggagt acggcgtctg ggtttatgcc
    38221 attctgtttt tgattctgtt ctgcgaaacg gggctggtgg taacgccatt cctgcctggt
    38281 gattcgctgc tgtttgttgc tggtgcattg gcgtcgctgg aaactaacga tctcaatgtc
    38341 catatgatgg tggtgcttat gttgattgcc gctattgtcg gtgatgcagt gaactatacc
    38401 attggacggt tatttggtga caaactgttc agtaacccgg attcgaaaat ctttcgtcgc
    38461 agttatcttg ataagactca tcagttttac gagagacatg gaggcaaaac gattattctc
    38521 gcccgttttg taccgatcgt cagaacgttc gccccgtttg ttgcgggaat gggccacatg
    38581 tcgtaccgtc atttcgccgc ttataacgtg atcggcgcac tgttgtgggt actgattttt
    38641 acctacgcgg gttatttctt cggcacgctc cccttcattc agagcaacct taagctgatg
    38701 attgttggca ttatttttgt ttcaatattg cctggggttt tcgaatttat tcgtcacaaa
    38761 cgcgctgcgg cacgcgccgc aaaataaaaa gtaactccgc ggttcgacca cttttttatc
    38821 caaagtttcg ggctgttatg ttttaatgtg caacattcat ggtctgttgg gggcaaaaat
    38881 ggcattatgc gtccccaaag ataaaactgg catcgaacca ggttcagaca gaaaggtccc
    38941 taatgagctg gattgaacga attaaaagca acattactcc cacccgcaag gcgagcattc
    39001 ctgaaggggt gtggactaag tgtgatagct gcggtcaggt tttataccgc gctgagctgg
    39061 aacgtaatct tgaggtctgt ccgaagtgtg accatcacat gcgtatgaca gcgcgtaatc
    39121 gcctgcatag cctgttagat gaaggaagcc ttgtggagct gggtagcgag cttgagccga
    39181 aagatgtgct gaagtttcgt gactccaaga agtataaaga ccgtctggca tctgcgcaga
    39241 aagaaactgg cgaaaaagat gcgctggtgg tgatgaaagg cactctgtat ggaatgccgg
    39301 ttgtcgctgc ggcattcgag ttcgccttta tgggcggttc aatggggtct gttgtgggtg
    39361 cacgtttcgt gcgtgccgtt gagcaggcgc tggaagataa ctgcccgctg atctgcttct
    39421 ccgcctctgg tggcgcacgt atgcaggaag cactgatgtc gctgatgcag atggcgaaaa
    39481 cctctgcggc actggcaaaa atgcaggagc gcggcttgcc gtacatctcc gtgctgaccg
    39541 acccgacgat gggcggtgtt tctgcaagtt tcgccatgct gggcgatctc aacatcgctg
    39601 aaccgaaagc gttaatcggc tttgccggtc cgcgtgttat cgaacagacc gttcgcgaaa
    39661 aactgccgcc aggattccag cgcagtgaat tcctgatcga gaaaggcgcg atcgacatga
    39721 tcgtccgtcg tccggaaatg cgcctgaaac tggcgagcat tctggcgaag ttgatgaatc
    39781 tgccagcgcc gaatcctgaa gcgccgcgtg aaggcgtagt ggtacccccg gtaccggatc
    39841 aggaacctga ggcctgataa ctgataaggg cagggccact ggctctgccc ttttgctatt
    39901 ctcaccgtaa cgaatcagcg gataccatga ttatcaaacg cactcctcaa gccgcgtcgc
    39961 ctctggcttc gtggctttct tatctggaaa acctgcacag taaaactatc gatctcggcc
    40021 ttgagcgcgt gagccaggtc gcggcgcgtc ttggcgttct gaaaccagcg ccatttgtgt
    40081 ttaccgttgc gggtacgaat ggcaaaggca ccacctgccg tacgctggag tcgattctga
    40141 tggcggcagg gtacaaagtg ggcgtctaca gttcaccgca tctggtgcgt tataccgagc
    40201 gcgtacgtgt gcagggccag gaattgccgg aatcggccca caccgcctct tttgcggaga
    40261 ttgaatcggc acgcggtgat atttccctga cctatttcga gtacggtacg ctgtcggcgt
    40321 tgtggctgtt caagcaggca caacttgacg tggtgattct ggaagtaggg ctgggcggtc
    40381 gtctggacgc aaccaatatt gtcgatgccg atgtcgcggt agtaaccagt attgcgctgg
    40441 atcataccga ctggctgggt ccagatcgcg aaagtattgg tcgcgagaaa gcaggcatct
    40501 tccgcagcga aaaaccggca attgtcggtg agccggaaat gccttctacc attgctgatg
    40561 tggcacagga aaaaggtgca ctgttacaac gtcggggcgt tgagtggaac tattccgtca
    40621 ccgatcatga ctgggcgttt agcgatgctc acggcacgct ggaaaatctg ccgttgccgc
    40681 ttgtcccgca accgaatgcc gcaacggcgc tggcggcact gcgtgccagc gggctggaag
    40741 tcagtgaaaa tgccattcgc gacgggattg ccagcgcaat tttgccggga cgtttccaga
    40801 ttgtgagcga gtcgccacgc gttatttttg atgtcgcgca taatccacat gcggcggaat
    40861 atctcaccgg gcgtatgaaa gcgctaccga aaaacgggcg cgtgctggcg gttatcggta
    40921 ttctacatga taaagatatt gccggaactc tggcctggtt gaaaagcgtg gttgatgact
    40981 ggtattgtgc gccactggaa gggccgcgcg gtgccacggc agaacaactg cttgagcatt
    41041 tgggtaacgg caaatcattt gatagcgttg cgcaggcatg ggatgccgca atggcggacg
    41101 ctaaagcgga agacaccgtg ctggtgtgtg gttctttcca cactgtcgca catgtcatgg
    41161 aagtgattga cgcgaggaga agcggtggca agtaagtttc agaatcggtt agtgggcacg
    41221 atcgtgctgg tggcgctggg ggtgattgta cttccagggc tgctggacgg gcagaaaaaa
    41281 cattatcagg atgagttcgc ggctatcccg ctggtgccga aagcgggcga tcgcgatgag
    41341 cctgatatga tgccagccgc cacccaggcg ttgccgacgc agccgccgga aggcgcagcg
    41401 gaagaggtgc gggcaggtga tgccgcagca ccgtcgctcg atccgaccac tattgcagcc
    41461 aataacaccg agtttgaacc ggaacctgca ccggtcgccc caccgaagcc gaaaccggtg
    41521 gagccgccta aaccgaaggt tgaagcacca cctgcgccga agccagaacc gaagccggtc
    41581 gtggaagaaa aagcagcgcc aacgggtaaa gcctatgttg tgcaactggg tgcgctgaaa
    41641 aatgccgata aagtgaatga gattgtcggt aagctgcgcg gtgccggtta tcgggtttat
    41701 acgtcgccat ccacgccagt gcagggtaaa attacccgta ttctggttgg gccggatgcc
    41761 tcgaaagata agctgaaagg ttcgctgggt gagttgaagc aactttctgg cttaagtggc
    41821 gtggtaatgg gctatacgcc gaattaatac agtcttgcct gatgcgacgc tggcgcgtct
    41881 tatcaggcct gggcagtgtt agaaccgtag gtcggataag gcgtttacgc cgcatccgac
    41941 acgcattgca cgatgccgca aaggcataaa aagtcgatgg cgttgaatat tttttcagcg
    42001 ccatttttat tgatgcgcgg gaaggaaatc cctacgcaaa cgttttcttt ttctgttaga
    42061 atgcgccccg aacaggatga cagggcgtaa aatcgtggga cacatatggt ctggattgat
    42121 tacgccataa tcgcggtgat tgctttttcc tctctggtta gcctgatccg cggctttgtt
    42181 cgtgaagcgt tatcgctggt gacatggggt tgtgctttct ttgttgccag tcattactac
    42241 acttacctgt cagtctggtt tacgggcttt gaagacgaac tggttcgaaa tggaattgcc
    42301 atcgcggtac tgtttatcgc taccctgatc gttggtgcta tcgtgaactt cgtgataggc
    42361 cagttggtgg agaaaacggg gttgtcaggc accgatcggg tgctgggcgt ctgtttcggt
    42421 gcgttgcgcg gtgtgttgat tgttgccgcc attctcttct ttctcgactc ctttaccggg
    42481 gtgtcgaaaa gcgaagactg gagcaaatca cagctgatcc cgcagttcag ttttatcatc
    42541 agatggtttt ttgattatct gcaaagctcg tcaagtttct tgcccagagc gtaagtgctc
    42601 tgagatgtgg cttaacgagg aaaaagacgt atgtgcggta ttgtcggtat cgccggtgtt
    42661 atgccggtta accagtcgat ttatgatgcc ttaacggtgc ttcagcatcg cggtcaggat
    42721 gccgccggca tcatcaccat agatgccaat aactgcttcc gtttgcgtaa agcgaacggg
    42781 ctggtgagcg atgtatttga agctcgccat atgcagcgtt tgcagggcaa tatgggcatt
    42841 ggtcatgtgc gttaccctac ggctggcagc tccagcgcct ctgaagcgca gccgttttac
    42901 gttaactctc cgtatggcat tacgcttgcc cacaacggca atctgaccaa cgctcacgag
    42961 ttgcgtaaaa aactgtttga agaaaaacgc cgccacatca acaccacttc cgactcggaa
    43021 attctgctta atatcttcgc cagcgagctg gacaatttcc gccactaccc gctggaagcc
    43081 gacaatattt tcgctgccat tgctgccaca aaccgcttaa tccgcggcgc gtatgcctgt
    43141 gtggcgatga ttatcggcca cggtatggtt gctttccgcg atccaaacgg gattcgtccg
    43201 ctggtactgg gaaaacgtga tattgacgag aaccgtacag aatatatggt cgcttccgaa
    43261 agcgtagcgc tcgatacgct gggctttgat ttcctgcgtg acgtcgcgcc gggcgaagcg
    43321 atttacatca ctgaagaagg gcagttgttt acccgtcaat gtgctgacaa tccggtcagc
    43381 aatccgtgcc tgtttgagta tgtatacttt gcccgcccgg actcgtttat cgacaaaatt
    43441 tccgtttaca gcgcgcgtgt gaatatgggc acgaaactgg gtgagaaaat tgcccgcgaa
    43501 tgggaagatc tggatatcga cgtggtgatc ccgatcccgg aaacctcgtg tgatatcgcg
    43561 ctggaaattg cgcgtattct tggcaaaccg taccgccagg gcttcgttaa aaaccgctat
    43621 gttggccgca ccttcatcat gccgggccag cagctgcgtc gtaagtccgt gcgccgtaaa
    43681 ctgaatgcca accgcgccga gttccgcgat aaaaacgtcc tgctggtcga cgactccatc
    43741 gtccgtggca ccacttctga gcagattatc gagatggcac gcgaagccgg agcgaagaaa
    43801 gtgtacctcg cttctgcggc accggaaatt cgcttcccga acgtttacgg tattgatatg
    43861 ccgagcgcca cggagcttat cgcccacggt cgcgaagtag atgaaattcg ccagatcatc
    43921 ggtgctgacg ggttgatttt ccaggatctg aacgatctga tcgacgccgt tcgcgctgaa
    43981 aacccggata tccagcagtt tgaatgctcg gtgttcaacg gcgtctacgt caccaaagat
    44041 gttgatcagg gctacctcga tttcctcgat actttacgta atgacgacgc caaagcagtg
    44101 caacgtcaga acgaagtgga aaatctcgaa atgcataacg aaggatgatg ccttcgctca
    44161 gggtgccggt ctggcaccct gacttgcaac tcctgccgaa atcctgcaaa gtctgcctgc
    44221 aagtctgaca gggcaactat ttatgaaacg actcattgta ggcatcagcg gtgccagcgg
    44281 cgcgatttat ggcgtgcgct tattacaggt tctgcgcgat gtcacagata tcgaaacgca
    44341 tctggttatg agccaggcag cgcgccagac cttatccctc gaaacggatt tttccctgcg
    44401 cgaagtgcag gcgttagccg atgtcacgca cgatgcgcgc gatattgccg ccagcatctc
    44461 ttccggttct ttccagacgc tggggatggt gattttaccc tgttcaatca aaaccctttc
    44521 cggcattgtc catagctata ccgatggttt actgacccgt gcggcagatg tggtgctgaa
    44581 agagcgtcgc ccgttggtgc tctgcgtgcg tgaaacacca ttgcacttag gccatctgcg
    44641 tttaatgact caggcggcag aaatcggtgc ggtgattatg cctcccgttc cggcgtttta
    44701 tcatcgccca caatcccttg atgatgtgat aaatcagacg gttaatcgtg ttcttgacca
    44761 gtttgcgata acccttcctg aagatctctt tgcccgctgg cagggcgcat aactccgctg
    44821 ttgccctgtt tcagggcaat tttgcaaccg cgatcaaatc ctcgacattt tgtttctgcc
    44881 attcaatcga aacgctgcga ttcaaccgct atacctgcta tcttcaactt caggacaata
    44941 atgcaacgtc ttattaacat atttaacgtt gaatgttact gttgtcgtca agatggcata
    45001 agacctgcat gaaagagcct gcaaatacac aacacaatac acaacataaa aaagccattt
    45061 tcacttgagg gttatgtatg aagaagtcga ttctcgctct gtctttgtta gtcggtctct
    45121 ccgcagcggc ttccagctat gcggcgctac cggagacggt acgtatcgga accgatacca
    45181 cctacgcacc gttctcatcg aaagatgcta aaggtgattt tgttggcttt gatatcgatc
    45241 tcggtaacga gatgtgcaaa cggatgcagg tgaaatgtac ctgggttgcc agtgactttg
    45301 acgcgctgat cccctcactg aaagcgaaaa aaatcgacgc tattatttcg tcgctttcca
    45361 ttaccgataa acgtcagcag gagattgctt tctccgacaa gctgtacgcc gcagattctc
    45421 gtttgattgc ggccaaaggt tcaccgattc agccaacgct ggattcactg aaaggtaaac
    45481 atgttggtgt gctgcaggga tcaacccagg aagcttacgc taacgagacc tggcgtagca
    45541 aaggcgtgga tgtggtggcc tatgccaacc aggatttggt ctattccgat ctggctgcag
    45601 gacgtctgga tgctgcgtta caagatgaag ttgctgccag tgaaggattc ctcaagcaac
    45661 ctgctggtaa agatttcgcc tttgctggct catcagtgaa agacaaaaaa tacttcggtg
    45721 atggcaccgg tgtagggcta cgtaaagatg atgctgaact gacggctgcc ttcaataagg
    45781 cgcttggcga gctgcgtcag gacggcacct acgacaagat ggcgaaaaag tatttcgact
    45841 ttaatgtcta cggtgactga tacgtcgctg ggaagctgta cctgatggaa tgatcatcat
    45901 ggtgcacgcc aggtttgttg cactatcgtg gtgcattgaa atgcatactt aagcattttt
    45961 aatgaaaaat aatacgtcta acggggcggg atattttgcc ttgatggtca attttatggc
    46021 acgataagtg taacaaacct gtaaatattc cctataaaaa gactgtcagt tgaggacatt
    46081 atgaaaaaac tggtgctatc gctctctctg gttctggcct tctccagcgc aactgcggcg
    46141 tttgctgcga ttccgcaaaa catccgcatc ggtaccgacc cgacctatgc gccatttgaa
    46201 tcaaaaaatt cacaaggcga actggttggc ttcgatatcg atctggcaaa ggaattatgc
    46261 aaacgcatca atacgcaatg tacgtttgtc gaaaatccgc tggatgcgtt aatcccgtcc
    46321 ttaaaagcga agaagattga cgccatcatg tcatcgcttt ccattacgga aaaacgtcag
    46381 caagaaattg ccttcaccga caaactgtac gctgccgatt ctcgtctggt ggtggcgaaa
    46441 aattccgaca ttcagccgac agtcgagtcg ctgaaaggca agcgggtagg cgtattgcag
    46501 ggcaccaccc aggagacgtt cggtaacgaa cattgggcac caaaaggcat tgaaatcgtc
    46561 tcgtatcagg ggcaggacaa catttattct gacctgactg ccggacgtat tgatgccgcg
    46621 ttccaggatg aggtcgctgc cagcgaaggt ttcctcaaac aacctgtcgg taaagattac
    46681 aaattcggtg gcccgtctgt taaagatgaa aaactgtttg gcgtagggac cggcatgggc
    46741 ctgcgtaaag aagataacga actgcgcgag gcactgaaca aagcctttgc cgaaatgcgc
    46801 gctgacggta cttacgagaa attagcgaaa aagtacttcg attttgatgt ttatggtggc
    46861 taatcgccat cggtgaaaga aagcccgctc ccttcgggta accggaggga gacaagaggc
    46921 ggtaattcac cacacacgac aggacaggca gcatgttgta tgggttttca ggtgttattt
    46981 tacagggtgc gctcgtcacg ctggagctgg ctatcagttc tgtagtgctc gctgtaatca
    47041 tcggtttaat tggcgctggc ggtaagctct cgcaaaatcg gctttcgggg ctgattttcg
    47101 aagggtacac cacgctgatt cgaggcgtgc cggacttggt gttgatgctg ctgattttct
    47161 acggtttgca gattgcacta aacacggtga cggaggcgat gggcgtcggg cagattgata
    47221 tcgatccgat ggtcgctggt attattactc tcggttttat ctacggtgct tattttaccg
    47281 aaacgttccg tggcgcgttt atggcagtgc cgaaagggca tatagaggcg gcgacggcgt
    47341 tcggttttac tcgtgggcaa gtgtttcggc ggatcatgtt tccggcgatg atgcgttacg
    47401 ccctgccagg cattggcaac aactggcagg tgatcctcaa atctaccgca ctggtttcgt
    47461 tactcggcct ggaagatgtg gtcaaagcca cccaactggc aggcaaaagt acctgggaac
    47521 cgttctattt cgccatcgtc tgtggcgtga tttacctggt tttcaccact gtttccaatg
    47581 gtgtgctgct gttccttgag cgccgctact ccgtgggtgt gaagagggct gacctgtgat
    47641 cgaaatctta catgaatact ggaaaccgct gctgtggacc gacggttatc gctttactgg
    47701 tgtggcgatc actctgtggc tgcttatttt gtcggtagtg ataggcagag tcctggcgct
    47761 gtttctggcg attggtcgtg tctccagtaa taaatacatc cagtttccaa tctggttatt
    47821 tacctatatt tttcgcggta cgccgctgta tgttcagttg ctggtgttct attccggcat
    47881 gtacacgctt gagattgtta agggaaccga attccttaac gctttcttcc gcagtggcct
    47941 gaactgtacc gtgctggcgc tgacgcttaa cacctgcgct tacactaccg agatttttgc
    48001 tggggcaatc cgttcggttc cgcatgggga aattgaagcc gccagagcct atggcttctc
    48061 gacttttaaa atgtatcgtt gcattatttt gccttctgcg ctgcgtattg cgttaccggc
    48121 atacagcaac gaagtgatcc tgatgctgca ctctactgcg ctggcgttta cggccactgt
    48181 gccggatctg ctgaaaatag cccgcgatat taacgccgcc acgtatcaac cttttaccgc
    48241 cttcggcatt gccgcggtgc tctatttaat catctcttat gtcctgatca gcctctttcg
    48301 cagagcggaa aaacgctggt tgcagcatgt gaaaccttct tcaacgcact gagaatacga
    48361 tgtccgagaa taaattaaac gttatcgatt tgcacaaacg ctacggcgaa catgaagtgc
    48421 tgaaaggggt ctcactgcaa gcgaatgccg gagatgtaat aagcatcatc ggatcgtcgg
    48481 gatcggggaa aagtaccttt ctgcgctgca ttaacttcct cgaaaaaccg agtgaagggt
    48541 cgatcgtggt caatggccag acgatcaatc tggtgcgcga caaagacggt caactcaaag
    48601 tcgccgataa aaatcaactg cgcttactgc gcacacgcct gacgatggta ttccagcact
    48661 tcaatctctg gagccatatg acggtgctgg aaaacgtcat ggaagcgccg attcaggtgt
    48721 tgggcctgag caagcaggaa gcgcgcgagc gggcggtgaa gtatctggca aaagtcggga
    48781 tagacgaacg tgcgcagggg aaatatccgg tgcatctttc cggcggtcag caacagcgtg
    48841 tttctatcgc gcgggcgctg gcgatggaac cggaagtttt actgtttgat gaacctacct
    48901 cggcgctcga tcctgaactg gtaggcgaag tgttacgtat tatgcagcaa ctggcagaag
    48961 aggggaaaac catggtggta gtgactcacg aaatgggctt tgctcgccat gtttctactc
    49021 acgtcatttt cctccatcag gggaaaatag aagaagaggg cgcgccggag cagttatttg
    49081 gcaacccgca aagccctcgt ctgcaacagt tccttaaggg atcgctgaaa taatttcctc
    49141 tccggtggct aacgctgccg gagtattttc atggggaatt tgcgagtcag cttccgctga
    49201 aatgtaaaag ttgcagtccg ctaaaaatac ttctgaattc gctgtccaaa gataaaaact
    49261 gcctgctgca ttgggtgtta atcgtggtta ttgtcgcgtc atcttaaatt tgaggggtga
    49321 cggaacgcca tgacaatatc gacaacttcc acgccgcatg atgcggtatt taaatctttt
    49381 ttacgccatc cagacaccgc gcgggatttt attgatattc atcttcccgc gccgctgcgc
    49441 aaactgtgtg atttaacgac gcttaaactg gaaccaaaca gttttattga tgacgacctg
    49501 cggcaatatt attccgacct cttgtggtct gtgaaaacgc aggagggagt gggttatatt
    49561 tatgtagtga tagagcacca aagtaagccg gaagaattaa tggcttttcg catgatgcgc
    49621 tattccatcg cggcgatgca aaaccatctg gatgcaggct ataaagagct tccattggtg
    49681 atcccaatgc tgttttatca tggttgcaga agtccttatc cttattcact ctgctggctt
    49741 gatgaatttg ccgagcctgc gattgcccgt aaaatatatt catcggcttt tccgttggtg
    49801 gatattaccg tggtgccgga tgacgagatt atgcaacatc gcaaaatggc gctgttggag
    49861 ttaattcaga aacatattcg tcagcgcgat ctgttgggat tagtcgacca aattgtttcg
    49921 ctgctagtta cagggaacac taatgacaga cagctaaaag ccctgtttaa ttatgtatta
    49981 caaacgggag atgcccagcg tttccgtgca tttattggtg agatagcaga acgcgcacca
    50041 caggaaaagg agaaactgat gaccattgct gacagattac gtgaagaagg gcggaatgat
    50101 ggattaattc ttggcaaacg cgaagaagcc ctgcgtattg ctcaggagat gctggataga
    50161 ggtttagacc gagagttagt tatgatggtg acccgacttt caccagacga tcttatcgcg
    50221 caaagccact aaccctg
//
LOCUS       DAEVZA010000021        47953 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEVZA010000021 DAEVZA010000000
VERSION     DAEVZA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.021"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(435..641)
                     /locus_tag="LAF92_001240"
     CDS             complement(435..641)
                     /locus_tag="LAF92_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBI9790284.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="LAF92_001241"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="LAF92_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBI9790285.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="LAF92_001242"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="LAF92_001242"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBI9790286.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4787
                     /gene="kdpB"
                     /locus_tag="LAF92_001243"
     CDS             2739..4787
                     /gene="kdpB"
                     /locus_tag="LAF92_001243"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBI9790287.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSHVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4796..5368
                     /gene="kdpC"
                     /locus_tag="LAF92_001244"
     CDS             4796..5368
                     /gene="kdpC"
                     /locus_tag="LAF92_001244"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBI9790288.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5361..8045
                     /gene="kdpD"
                     /locus_tag="LAF92_001245"
     CDS             5361..8045
                     /gene="kdpD"
                     /locus_tag="LAF92_001245"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HBI9790289.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8042..8719
                     /gene="kdpE"
                     /locus_tag="LAF92_001246"
     CDS             8042..8719
                     /gene="kdpE"
                     /locus_tag="LAF92_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBI9790290.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
     gene            8994..9098
                     /locus_tag="LAF92_001247"
     CDS             8994..9098
                     /locus_tag="LAF92_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBI9790291.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9409..11607
                     /gene="speF"
                     /locus_tag="LAF92_001248"
     CDS             9409..11607
                     /gene="speF"
                     /locus_tag="LAF92_001248"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBI9790292.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11604..12923
                     /gene="potE"
                     /locus_tag="LAF92_001249"
     CDS             11604..12923
                     /gene="potE"
                     /locus_tag="LAF92_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBI9790293.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMKLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12979..14619)
                     /gene="pgm"
                     /locus_tag="LAF92_001250"
     CDS             complement(12979..14619)
                     /gene="pgm"
                     /locus_tag="LAF92_001250"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBI9790294.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14645..15190)
                     /gene="seqA"
                     /locus_tag="LAF92_001251"
     CDS             complement(14645..15190)
                     /gene="seqA"
                     /locus_tag="LAF92_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBI9790295.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15375..16139
                     /gene="ybfF"
                     /locus_tag="LAF92_001252"
     CDS             15375..16139
                     /gene="ybfF"
                     /locus_tag="LAF92_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI9790296.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16279..16572
                     /gene="ybfE"
                     /locus_tag="LAF92_001253"
     CDS             16279..16572
                     /gene="ybfE"
                     /locus_tag="LAF92_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBI9790297.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16712..17242
                     /gene="fldA"
                     /locus_tag="LAF92_001254"
     CDS             16712..17242
                     /gene="fldA"
                     /locus_tag="LAF92_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBI9790298.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17452..17538
                     /gene="uof"
                     /locus_tag="LAF92_001255"
     CDS             17452..17538
                     /gene="uof"
                     /locus_tag="LAF92_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBI9790299.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17531..17977
                     /gene="fur"
                     /locus_tag="LAF92_001256"
     CDS             17531..17977
                     /gene="fur"
                     /locus_tag="LAF92_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBI9790300.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18061..18387)
                     /locus_tag="LAF92_001257"
     CDS             complement(18061..18387)
                     /locus_tag="LAF92_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790301.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18437..19843)
                     /gene="chiP"
                     /locus_tag="LAF92_001258"
     CDS             complement(18437..19843)
                     /gene="chiP"
                     /locus_tag="LAF92_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBI9790302.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20289..21953)
                     /gene="glnS"
                     /locus_tag="LAF92_001259"
     CDS             complement(20289..21953)
                     /gene="glnS"
                     /locus_tag="LAF92_001259"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBI9790303.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVADKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22156..24102)
                     /gene="nagE"
                     /locus_tag="LAF92_001260"
     CDS             complement(22156..24102)
                     /gene="nagE"
                     /locus_tag="LAF92_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBI9790304.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            24435..25235
                     /gene="nagB"
                     /locus_tag="LAF92_001261"
     CDS             24435..25235
                     /gene="nagB"
                     /locus_tag="LAF92_001261"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBI9790305.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25295..26443
                     /gene="nagA"
                     /locus_tag="LAF92_001262"
     CDS             25295..26443
                     /gene="nagA"
                     /locus_tag="LAF92_001262"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBI9790306.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26452..27672
                     /gene="nagC"
                     /locus_tag="LAF92_001263"
                     /gene_synonym="nagR"
     CDS             26452..27672
                     /gene="nagC"
                     /locus_tag="LAF92_001263"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBI9790307.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27720..28472
                     /gene="nagD"
                     /locus_tag="LAF92_001264"
     CDS             27720..28472
                     /gene="nagD"
                     /locus_tag="LAF92_001264"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBI9790308.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28729..30393
                     /gene="asnB"
                     /locus_tag="LAF92_001265"
     CDS             28729..30393
                     /gene="asnB"
                     /locus_tag="LAF92_001265"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBI9790309.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            30774..30850
                     /locus_tag="LAF92_001266"
     tRNA            30774..30850
                     /locus_tag="LAF92_001266"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30808..30810,aa:Met,seq:cat)
     gene            30859..30943
                     /locus_tag="LAF92_001267"
     tRNA            30859..30943
                     /locus_tag="LAF92_001267"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30893..30895,aa:Leu,seq:tag)
     gene            30967..31041
                     /locus_tag="LAF92_001268"
     tRNA            30967..31041
                     /locus_tag="LAF92_001268"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30999..31001,aa:Gln,seq:ttg)
     gene            31076..31150
                     /locus_tag="LAF92_001269"
     tRNA            31076..31150
                     /locus_tag="LAF92_001269"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31108..31110,aa:Gln,seq:ttg)
     gene            31166..31242
                     /locus_tag="LAF92_001270"
     tRNA            31166..31242
                     /locus_tag="LAF92_001270"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31200..31202,aa:Met,seq:cat)
     gene            31291..31365
                     /locus_tag="LAF92_001271"
     tRNA            31291..31365
                     /locus_tag="LAF92_001271"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31323..31325,aa:Gln,seq:ctg)
     gene            31403..31477
                     /locus_tag="LAF92_001272"
     tRNA            31403..31477
                     /locus_tag="LAF92_001272"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31435..31437,aa:Gln,seq:ctg)
     gene            complement(31631..32806)
                     /gene="ubiF"
                     /locus_tag="LAF92_001273"
     CDS             complement(31631..32806)
                     /gene="ubiF"
                     /locus_tag="LAF92_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBI9790310.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            32952..34376
                     /gene="miaB"
                     /locus_tag="LAF92_001274"
     CDS             32952..34376
                     /gene="miaB"
                     /locus_tag="LAF92_001274"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBI9790311.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34529..35569
                     /locus_tag="LAF92_001275"
     CDS             34529..35569
                     /locus_tag="LAF92_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBI9790312.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35566..36033
                     /gene="ybeY"
                     /locus_tag="LAF92_001276"
     CDS             35566..36033
                     /gene="ybeY"
                     /locus_tag="LAF92_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBI9790313.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36123..37001
                     /gene="corC"
                     /locus_tag="LAF92_001277"
     CDS             36123..37001
                     /gene="corC"
                     /locus_tag="LAF92_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBI9790314.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37026..38564
                     /gene="lnt"
                     /locus_tag="LAF92_001278"
     CDS             37026..38564
                     /gene="lnt"
                     /locus_tag="LAF92_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBI9790315.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALVLVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(38642..40519)
                     /locus_tag="LAF92_001279"
     CDS             complement(38642..40519)
                     /locus_tag="LAF92_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBI9790316.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLFSSVFLHSSFSHLLMNMFALLVVGAVAERILGKWRLLI
                     IWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAALATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQLQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            40974..41882
                     /gene="gltI"
                     /locus_tag="LAF92_001280"
     CDS             40974..41882
                     /gene="gltI"
                     /locus_tag="LAF92_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBI9790317.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            42052..42792
                     /gene="gltJ"
                     /locus_tag="LAF92_001281"
     CDS             42052..42792
                     /gene="gltJ"
                     /locus_tag="LAF92_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBI9790318.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            42792..43466
                     /gene="gltK"
                     /locus_tag="LAF92_001282"
     CDS             42792..43466
                     /gene="gltK"
                     /locus_tag="LAF92_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBI9790319.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            43466..44191
                     /gene="gltL"
                     /locus_tag="LAF92_001283"
     CDS             43466..44191
                     /gene="gltL"
                     /locus_tag="LAF92_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBI9790320.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            44309..45244
                     /gene="rihA"
                     /locus_tag="LAF92_001284"
     CDS             44309..45244
                     /gene="rihA"
                     /locus_tag="LAF92_001284"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBI9790321.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            45328..46998
                     /gene="hscC"
                     /locus_tag="LAF92_001285"
     CDS             45328..46998
                     /gene="hscC"
                     /locus_tag="LAF92_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HBI9790322.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(47058..>47953)
                     /gene="djlC"
                     /locus_tag="LAF92_001286"
     CDS             complement(47058..>47953)
                     /gene="djlC"
                     /locus_tag="LAF92_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBI9790323.1"
                     /translation="FALERCCRYHPDYVTAFLAMEGPWFIPDDAKLHRKLLRWYSSVQ
                     TGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLAELCAYWESYPSTQADN
                     LLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRLLWAEILHSGKLS
                     PLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVLEAFPKQEMLARL
                     NTRLEGGDAHPLEAIITRLLLTKVKLEPEDEDVDEPTPENHEEKNDEGEKPQSITSII
                     KISLTVLVIGYALGKIAMLFS"
BASE COUNT        11450 a        12287 c        12418 g        11798 t
ORIGIN      
        1 gatgtcggtt tcaccgggaa ggacctttgc accgagcagg ggattgaccg gatggccgga
       61 cgtcactccg ccggggcacc ccgaacaggc cacaccggtt ggggcaccga tgcgcacccc
      121 ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg tcgccctgac gcgccgccgg
      181 ttttccgctc atctgctcat ccttgtttta tcgggttatt tgtctgctga caggcgaaac
      241 cctacagatt tataagaaag ccttaagtga gatatctgtc agataagaac aattaggaaa
      301 atatttatat atatttcatg aatatatgtt tcgattggta taacgatgtc taaatattcc
      361 tctgaacatc ttcctgatac aaaaaaccgc cagcgtttcc gctggcggtt ttcaatgtat
      421 tcaaaactca gcaatcaata aaaatcacca gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcga tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata agtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatggcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcatcct gtaattaagt tacacaaaag ttaaattaat actaaacatt agttaaatca
      841 tggcttttgc catttttata ctttttttac accccgcccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctaccctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat aaccggcgta ttgctggtgt ttttattact gggttatctg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgatcg ccacgttttt
     1081 actggtgtta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttacagcg gtgaaaccac gttgagctat ttcagccaga tggctggctt
     1441 aacggtgcaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccctcatccg
     1501 tgcgtttacc cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctcgtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgctgcaa aactttcagc cttatcaggc tgtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg gggcctgtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacggcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggtgatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcttc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggtggca tggtgccgat gtggctgatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt taccccgacg ctggtgctga tgggcgcggc
     2341 gctggcaatg atgaccgacg ccggacgtag cgccatgctc aaccctggcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcatccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactccccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gcggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatccct gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggccgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat gttgaggcta
     3961 acggcggtca cttccctacc gatgttgatc aaaaagtcga tcaggttgcg cgtcaggggg
     4021 ccacgccgct ggtggtggtg gaaggttccc atgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaaatgggc attaaaacgg
     4141 tgatgattac cggcgataac cgtctgactg ccgccgccat tgctgcggaa gcgggcgtcg
     4201 atgattttct cgccgaagcg acaccggagg ccaagctggc attgattcgt cagtatcagg
     4261 cggaaggccg tttggtggcg atgaccggcg acggcaccaa cgatgctcca gcgctggcgc
     4321 aggcagatgt cgcggtggcg atgaactccg gtactcaggc ggcgaaagaa gcgggcaata
     4381 tggtcgatct cgactctaac ccgaccaagt tgatcgaggt ggtacatatt ggcaaacaga
     4441 tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg gcgaaatact
     4501 tcgccattat tccggcggcg ttcgcggcaa cgtatccgca gttaaatgcg ctgaacatca
     4561 tgcgccttca ttcgcccgac tccgccattc tcagtgcggt cattttcaac gccttgatta
     4621 tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt accgcttctg
     4681 ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg ccgtttattg
     4741 gtatcaaagt cattgattta ctgctgaccg tttgcggtct ggtgtgaggt ttaccatgcg
     4801 tggcttacgt ccggcattat cgacatttat ctttctgtta ttgattactg gcggcgttta
     4861 cccgctgctg accaccgtgc tggggcaatg gtggtttccc tggcaggcca acggttcgtt
     4921 gattcgtgaa ggtgatacgg tgcggggttc ggcattaatc gggcagaatt ttaccggcaa
     4981 cggctatttt cagggtcgcc cgtcggcaac ggcagaaatg ccctataatc cacaggcttc
     5041 tggcgggagc aatctggcgg tcagtaaccc tgagctggat aaacaaatag ccgcacgcgt
     5101 tgctgcatta cgggccgcta acccggatgc cagtacgaac gttccggttg aactggtgac
     5161 ggcatcggca agcgggctgg acaataatat caccccgcaa gcggcggtct ggcaaatccc
     5221 acgcgtggcg aaagcgcgta atctcagcgt tgaacagctc acgcaactga tcgcaaaata
     5281 cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg ttgaactcaa
     5341 tctggcgctg gataaacttg atgaataacg aacccttacg tcccgacccc gatcgtctgc
     5401 tggaacaaac tgccgcgccg catcggggga agctgaaagt tttcttcggt gcctgtgcag
     5461 gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg caagggctgg
     5521 atattgtggt tggcgtggta gaaacccatg ggcggaaaga taccgccgcc atgcttgaag
     5581 ggctggctgt tctgccgccc aaacgccagg cgtaccgtgg gcggcatatc agcgagtttg
     5641 atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa ctggcgcaca
     5701 gtaatgcgcc aggttctcgt catcctaaac gctggcagga tatcgaagaa ctgctggaag
     5761 ctggcattga tgttttcaca accgtcaacg ttcagcatct ggaaagtctg aatgatgtgg
     5821 tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt ttcgatgccg
     5881 ccgacgacgt agtgctggtg gacttgcccc cggacgatct gcgccagcgg ctgaaagaag
     5941 gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc cgcaaaggca
     6001 atctgatcgc cctgcgtgaa ctggcactgc gccgtactgc cgatcgcgta gatgagcaaa
     6061 tgcgcgcctg gcgggggcat cctggtgaag agaaagtgtg gcacacgcgc gacgcgatcc
     6121 ttttatgcat cgggcataac accggcagtg agaaactggt ccgcgcggcg gcgcggttgg
     6181 cctcgcgcct gggtagcgtc tggcacgcgg tgtatgttga aacccctgcc ctgcatcgct
     6241 taccggaaaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg
     6301 cggagacggc tacactttct gatccagcgg aagaaaaagc agtagtgcgt tatgcccgtg
     6361 aacataatct cggcaagatt attctcggtc gcccggcctc gcgccgctgg tggcgtcggg
     6421 aaacgtttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc
     6481 ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctct tttaaagaca
     6541 agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcactt
     6601 taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc
     6661 ttggcgtggt ggtggtggcg ctattttatg gacgctggcc ttcggtggtt gctaccgtca
     6721 ttaatgtggt gagtttcgat ctctttttta tcgccccacg cggcacgctc gctgtctctg
     6781 atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt cgggctggtg atcgggaacc
     6841 ttactgcggg tgtgcgttat caggcgcggg tagcccgtta ccgcgagcaa cgcacacggc
     6901 acttatatga aatgtcgaaa gctctggcgg tgggccgcag tccgcaggac atcgccgcca
     6961 ccagcgaaca atttattgcc tccacgtttc atgcccgcag tcaggtgttg ttgcccgatg
     7021 acaacggtaa attgcagccg ttaacacatc cgcaaggaat gaccccgtgg gacgatgcca
     7081 tcgcacagtg gagttttgat aaaggcctgc ctgcgggtgc gggcaccgac acgttacccg
     7141 gcgtgccgta ccagattttg ccgctaaaaa gcggcgagaa aacctacgga ctggtggtgg
     7201 tggaaccggg gaatctgcgt cagttgatga tcccggagca gcagcgcctg ctagagacgt
     7261 ttacgctgtt agtcgccaat gcgtttgagc ggctgacgct gaccgccagt gaagaacagg
     7321 cgcggatggc aagcgaacgt gaacagatcc gcaacgccct gctggcggcg ctttcgcatg
     7381 atttacgcac gccgcttacg gtgctgtttg gtcaggcaga aatcttaacg ctcgatctgg
     7441 caagcgaagg atcgccccac gcccgccagg ccagcgagat tcgtcagcat gtgctgaaca
     7501 ctacccgact ggtgaataat ctactggata tggcgcgaat tcagtccggc ggctttaatt
     7561 tgaagaaaga gtggttaacg ctggaagaag tagtcggcag cgcgctgcaa atgctggaac
     7621 cgggtttatc gtcgcccatc aatctttctc tgccagaacc gctgacctta atccacgttg
     7681 acgggccact ctttgaacgg gtgctgatta atctgctgga gaacgcggtg aaatatgcgg
     7741 gtgcgcaggc cgaaattggt atcgatgccc acgttgaggg cgaaaatcta caactggatg
     7801 tctgggataa cggccccggt cttccgccag gccaggagca gacgatattt gataagtttg
     7861 ctcgcgggaa taaagagtcg gcagtaccgg gggtagggct tggactggca atttgtcggg
     7921 cgatagtgga tgtacacggg ggcactatta ccgcgttcaa ccgaccggaa ggtggtgcct
     7981 gttttcgtgt tacacttccc cagcaaaccg ctcctgaact tgaagacttt catgaggata
     8041 tgtgacaaac gttctgattg ttgaagatga acaggctatt cgtcgctttc tgcgcacggc
     8101 gctggagggc gacgggatgc gcgtctatga ggccgaaacg ctgcaacgcg gcttgctgga
     8161 agcggctacc cgtaagccag atttgattat tctcgatctc ggcctgcccg atggtgatgg
     8221 gattgagttt atccgcgacc tgcgccagtg gagcgcggtg ccggtgattg tgctttccgc
     8281 acgcagcgaa gagagcgaca aaatcgccgc gctggatgcc ggagcggatg attatctgag
     8341 taagccgttt ggcattggcg aattgcaggc ccgtctgcgc gtcgctttac gccgccactc
     8401 cgccaccgcc gcgcccgatc cgctggtgaa attttccgat gttaccgtcg atttagccgc
     8461 ccgcgtgatt caccggggtg aggaagaggt gcatctcacg ccgattgagt tccgcctgct
     8521 ggcggtgctg ctgaataatg ccgggaaagt gctcacccag cgccagttac tcaatcaggt
     8581 gtgggggcca aacgcggtcg aacacagtca ctatttgcgt atttatatgg gacatctgcg
     8641 acaaaaactg gaacaggatc ccgctcgccc ccgccatttc attactgaaa ccggtattgg
     8701 gtatcggttt atgccatgaa tgtttattca ataaatgcgc ttaaataatt attaatttat
     8761 tattaaacct gccaaaaata ttattatttg gcaggtttaa tttcttaacg caaatataaa
     8821 acacaaaatt acaacattta aataacaaaa cagcatctaa tatggcgcta ttcataatta
     8881 atgatttatt atttggggta tgatcgtttt ttgttgatct tcttcacaga ttatagccat
     8941 ttcatggata gaataactct accttcaact gacacagcaa gaggtaaagg taaatggaaa
     9001 ataataaccg cttaatgcct catataaggc ggacaaccca tatcatgaag tttgcccatc
     9061 gtaatagttt tgactttcat ttctttaatg cccgctagtc ttctgactaa agggcacccc
     9121 aacgtacagg tctccctgac tttaagcatt acaggttaat acctgtattc ctcggtgctc
     9181 atatactgct aaccctttta aactctaaat aattcgagtc gcagcacttg caacttgagg
     9241 tatgacgagt atagccagtt accgggctgg tctgggttat tgcatctgca aaaagcaaac
     9301 tactgattta tttatcagcg gtggagcttt gctttttttc cggcgtgatc gatttctcct
     9361 ttgagaaatt gaggacctgc tattacctga aataaagaga tgaacaaaat gtcagaatta
     9421 aaaattgccg ttagtcgttc ttgcccggat tgtttttcca ctcatcgtgc atgcgtgaat
     9481 atagacgaaa gtaattatat tgacgttgcc gccattattt tatcagtcag tgatgttgaa
     9541 cgtggaaaac tcgatgaaat agacgctact ggctatgaca ttcctgtttt tattgcaacg
     9601 gaaaatgaag aacgtgtacc cgcagaatat ctgccacgta tttctggtgt cttcgaacat
     9661 tgcgaatcac gtaaagagtt ttatggacgt cagttagaaa ccgctgccag ccattatgaa
     9721 acgcaactgc gcccgccctt cttccgcgca ctggtcgatt atgttaatca gggcaatagt
     9781 gcttttgact gccctggtca tcaggggggt gagtttttcc gtcgccatcc ggcaggcaat
     9841 cagttcgtgg aatactttgg cgaaatgctc ttccgctctg acttgtgtaa tgctgacgta
     9901 gcaatgggcg atctgctgat tcatgaaggt gcgccatgta ttgcacaaca gcatgcggct
     9961 aaagtcttta atgccgataa aacctatttc gttttaaacg gtacttcatc ttctaataaa
    10021 gtggtcttaa acgcattgct gacgccgggc gatctggtgt tgtttgaccg caataaccat
    10081 aaatctaacc accacggtgc gttgctacag gctggcgcga caccggttta tctggaaacc
    10141 gcacgtaacc cgtatggttt tatcggtggt attgacgctc actgttttga agaaagttac
    10201 ctgcgtgagc tgattacaga agttgcaccg cagcgggcaa aagaggctcg tcctttccgc
    10261 ctcgccgtta ttcagctagg cacttacgac ggtacgattt ataacgcccg tcaggtggtg
    10321 gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt tggctatgag
    10381 caatttatcc cgatgatggc tgactgttcg ccgctgttgc tggatcttaa tgagaacgat
    10441 ccgggtattc tggttacgca atccgtacat aaacagcagg ctggtttttc tcaaacttca
    10501 caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc
    10561 atgaacaacg cctttatgat gcacgcctcc accagcccgt tctatccgct gtttgccgca
    10621 ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt
    10681 gtggtaaatg gcattaatgc ccgcaaactg atcctcgata actgtcagca tattcgtccg
    10741 ttcgtacctg aactggtgga tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg
    10801 gttgatctgc gcttcttcca gtttgtacca ggggaacact ggcattcttt tgaaggctat
    10861 gcagagaatc aatactttgt cgatccttgc aaactgttgc tgacaacccc aggtattgat
    10921 gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg
    10981 cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaact ccatcctctt cctgctgact
    11041 ccggcagaag atatggccaa gcttcagcaa cttgttgccc tgctggtacg cttcgaaaaa
    11101 ctgcttgagt ccgacgcgcc attagcagaa gtgctgcctt ccatctacaa acagcatgaa
    11161 gagcgctacg ccggttatac cctgcgtcag ttgtgtcaag aaatgcatga tttgtatgcc
    11221 cgtcacaacg tgaaacaact gcaaaaagag atgttccgta aggaacactt cccacgcgta
    11281 agcatgaatc cgcaagaagc caactacgcc tatttacgcg gtgaagtgga gctggttcgt
    11341 ctgccggatg cagaaggccg tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg
    11401 ctgtgcgttg ttccgggtga aatctggggt ggtgctgttc tgcgttactt cagcgctctg
    11461 gaagaaggga tcaacctgtt gccaggtttt gcaccggagc ttcagggtgt ctatatcgaa
    11521 gaacatgatg gtcgtaagca agtttggtgc tatgtcatca agcctcgtga tgcgcaaagt
    11581 accctgttga aaggggaaaa attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc
    11641 agttaaccat actgacgatg gtcaacatga tgggctccgg tatcatcatg ctgccgacaa
    11701 agcttgccga agttgggaca atttcaatta tctcctggct ggtgacagcc gtaggctcaa
    11761 tggcactggc atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca ggtggtatgg
    11821 gcggttatgc cgagtatgct ttcggtaagt ccggcaattt tatggcgaac tacacctacg
    11881 gtgtctcgct gctgattgct aacgtcgcga ttgctatttc ggcggttggt tacggcaccg
    11941 aactgctcgg cgcgagtttg tcgccagtgc agattggtct tgcgaccatc ggagtgctgt
    12001 ggatttgtac cgtggctaac tttggtggtg cgcgcattac cgggcaaatc agtagcatta
    12061 ctgtgtgggg ggtcattatt ccggtggtag gcctgtgcat tatcggctgg ttctggttta
    12121 gcccgacgct ttacgttgat tcctggaatc cgcatcatgc accgttcttc agtgcagtag
    12181 gttcttccat cgctatgaag ctgtgggctt ttctcggtct ggagtctgcg tgtgcgaata
    12241 ctgatgtagt ggaaaacccg gaacgtaatg tgccaatcgc ggtactcggc ggtacgttag
    12301 gtgcggcggt gatttatatc gtctccacca acgtgattgc agggattgtg cctaatatgg
    12361 agctggcaaa ttcaacggca ccatttggcc tggccttcgc acagatgttc acaccagaag
    12421 tgggtaaagt cattatggcg ctgatggtga tgtcctgctg cggttcgctg cttggctggc
    12481 agttcaccat tgcccaggtg tttaaatctt catctgatga aggctacttc cctaaaattt
    12541 tctcccgtgt aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt gtgattatcc
    12601 agagcgggtt ggcgctgatg acgattagcc cgtcgctaaa cagtcagttc aacgtgctgg
    12661 ttaacctggc cgtggtgacc aatatcattc cgtatattct gtcaatggcg gcattagtca
    12721 ttattcagaa ggtcgctaat gtgcccccct caaaagcgaa agttgcaaac tttgttgctt
    12781 ttgttggcgc gatgtatagc ttttatgcgc tgtactcatc cggggaagaa gccatgctgt
    12841 acggttccat cgtgaccttc cttggttgga cactgtatgg tctggtatca ccacgctttg
    12901 aactgaaaaa taaacacggt taaaaaagca aagtaaaaaa gggcgatctt gcgaccgccc
    12961 tttttttatt aaatgtgttt acgcgttttt cagaacttcg ctaacaatct caaccgcttc
    13021 tttctcaatc tgcttgcgat gttcttcacc gaggaagctt tcgcagtaga tcttatatgc
    13081 gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg tcggtcatca ctttcagacc
    13141 gccgatagaa gcaccattgc ccggtgccgc cgtcaggcgc gcggtgatcg ggtcgcctgc
    13201 aagggtgctg gcgctcacca tttccggaga cagcttagac agcgccgctt tttgtgcgga
    13261 agtcgcggct gcctgcaaac ggttgtagct cggcgcacca aagcgttttg ccagttcgtt
    13321 gtagtgttcc tgcgggttct taccggtgac agcggtgatt tccgccgcca gcagacacat
    13381 aatgatgccg tctttgtcgg tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc
    13441 tgcgctctct tcgccgccaa agccgaagct gccgtcgaac agaccatcaa caaaccattt
    13501 gaaacctacc gggacttcta ccagcttacg gcccaagtca ttgaccacac ggtcgatcat
    13561 cgcagaagaa accagcgttt taccgacagc aacatctttg ccccactgcg gacgatgctg
    13621 gaacaggtag ttgatcgcca ccgccaggta gtggttcgga ttcatcaaac ctgccggagt
    13681 gacgataccg tgacggtcat aatccgggtc gttagcaaac gccagatcga acttatcacg
    13741 cagtgccagc aggcccgcca tcgcacactc ggaggagcag tccatacgga tcgcgccgtc
    13801 tttatcaagg tgcataaagc ggaaggtttg atcgacctga tcgttaacga tggtcaggtt
    13861 gaggttgtaa tactcgccaa tacgcttcca gtattcgata ccggaaccgc ccagcggatc
    13921 aacgcccagc gtcaggcccg ctttctgaat tgccgccata tcaacgatat cggccagacc
    13981 ttccacaaac ggctgcacca gatcctgctc ttttacatga ccggatgcca tcgcttcgtc
    14041 gagggagata cgcttcacgc ctttcaggcc atcggccatc agtgcgttgg ccctgtcttc
    14101 caccactttg gtgacgttgg tatcagccgg gccaccattt ggcgggttgt acttgatacc
    14161 accatcttcc ggcgggttat gggacggtgt aatcacgata ccgtctgcca gcgggccacc
    14221 ttttttattg tgaaccagga tggcattgga aatggcaggt gtcggagtga agccattgtt
    14281 ttcctgcaca atgacatcaa cgccgttcgc tgccagcact tccagtacgg aaatgaacgc
    14341 aggctcggac agggcgtgag tatctttacc cacatagcaa gggccagtga tgccgttttt
    14401 cgcacgttct tcagcaattg cctgagcgat cgccagaatg tgcggctcgt taaagctgtg
    14461 gcgcgctgca ctgccacggt gaccggaagt accgaatttc accgcgtgct ccgcattccc
    14521 tgcttctggt ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact
    14581 ctgttgtgca ggttggcctg cacgattgtg gattgccatt gctttgtcct ttgtctgcaa
    14641 cgttttagat agttccgcaa accttctcaa tcaattccgc cgggaattgc atcgactgca
    14701 tgatgtgttc gatcatgctg catttacggc cggtgttggt gttggtgatc acccaatacg
    14761 gcgtgcctgg cacatgtttc ggcttggtct gattaccatt tttcagcagc gtttgttcat
    14821 ctgccgcaaa gtaaacgcgt gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct
    14881 gggcgtcaag agaatatagt gtagacaaca gcagcataaa gcgattgacc gctcgctttt
    14941 gctctgcgta ttcatccgaa agcagaagtt cacgcattgc gcgaaccttg tctttaatcg
    15001 ttttgaccgg cttcgcttcg acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg
    15061 gagcagcagg ctgtgatgcg gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc
    15121 tctcgccgat atgcttagtg tggctggcaa tatagctgta gagttcatca tcaacttcaa
    15181 tcgttttcat cttaatccag tgcagtgtct tagctgaata caagtcgcca ggagtatatg
    15241 aataaatccc ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg gaataaacct
    15301 tatttgttgc agccctgtgg cacaatggtc aacataatac cctaacccga caacgcgaaa
    15361 aaaagaactt tgccatgaaa ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata
    15421 attctcccat cgttcttgtc catggtctgt ttggtagcct cgacaacctt ggcgtactgg
    15481 ctcgcgatct ggtaaacgat cacaatatca tccaggttga tatgcgtaac cacggtcttt
    15541 caccgagaga tccggtaatg aattacccgg cgatggcgca ggatcttgtt gatactttgg
    15601 atgcacagca gatcgacaaa gcaacattta tcggtcactc catgggcggt aaagcggtaa
    15661 tggcacttac tgcactggcc cccgatcgca tcgataaact ggtggcgatc gatatcgcac
    15721 cggtcgacta tcacgtacgc cgtcatgatg agattttcgc ggctatcaac gcggtcagtg
    15781 aatcggacgc acaaactcgc cagcaagcag cagcaataat gcgccagcat cttaatgaag
    15841 aaggggtgat tcagtttctg ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc
    15901 cggtattgtg ggatcagtat ccgcatattg tgggttggga gaaaatcccg gcatgggatc
    15961 accctgccct gtttatccct ggcggcaatt ctccgtatgt tagcgagcag taccgtgatg
    16021 atttactggc acaatttcca caggcacggg cgcatgtgat tgcaggcgcg ggtcactggg
    16081 tccatgctga aaaaccggat gcggtattac gcgctatccg tcgctatctc aatgattaac
    16141 gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc
    16201 atcgcgctga tgtattatgg cgcgctatcc atccgggcag aggcctggct gattgtttcc
    16261 cccgaagtca ccaagatcat ggccaaagaa caaacggacc gtacgacatt agatctgttc
    16321 gcgcacgagc gtcgaccggg acgaccgaaa actaatccgc tttcgcgcga tgaacagctg
    16381 cgtattaata aacgcaacca gctaaaacgc gacaaagtac gtggccttaa gcgtgtcgaa
    16441 ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc tggcggagtc gcgcaatatg
    16501 agccgtagcg aactgatcga agagatgtta atgcagcaac tggcggcgct gcgtagtcag
    16561 ggcatcgttt aaatttccac tttcatgtag cacagtgtgc agtcctgctc gtttgctgat
    16621 tccgcaagac tgcctgttct gctatgattg cctttatccg tgggcaattt tccaccccca
    16681 tttcaataag tttcaagagg ttatttcact catggctatc actggcatct ttttcggcag
    16741 cgacaccggt aataccgaaa atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt
    16801 tgccgatgtc catgacattg caaaaagcag caaagaagat ctggaagctt atgacattct
    16861 gctgctgggc atcccaacct ggtattacgg cgaagcgcag tgtgactggg atgacttctt
    16921 cccgactctc gaagagattg atttcaacgg caaactggtt gcgctgtttg gttgtggtga
    16981 ccaggaagat tacgccgaat atttctgcga cgcattgggc accatccgcg acatcattga
    17041 accgcgcggt gcaaccatcg ttggtcactg gccaactgcg ggctatcatt tcgaagcatc
    17101 aaaaggtctg gcagatgacg accactttgt cggtctggct atcgacgaag accgtcagcc
    17161 ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag atttctgaag agttgcatct
    17221 cgacgaaatt cttaatgcct gatgtgatgc ggcgtagact catgtctacg ccgtattaat
    17281 agataatgcc aatcaaaata attgctacaa atttgtaact tttgctgttg tacctgtaca
    17341 atgtcccggt gttcaagtgg ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta
    17401 acaatatttg ccagggactt gtggttttca tttaggcgtg gcaattctat aatgatacgc
    17461 attatctcaa gagcaaattc tgtcacttct tctaatgaag tgaaccgctt agtaacagga
    17521 cagattccgc atgactgata acaataccgc cctaaagaaa gctggcctga aagtaacgct
    17581 tccacgttta aaaatcctgg aagttcttca ggagccggac aaccatcacg tcagtgcgga
    17641 agatttatac aaacgtctga tcgatatggg tgaagaaatt ggtctggcta cggtatatcg
    17701 cgtactgaac cagtttgacg acgctggtat cgtcacccgc cacaattttg aaggcggtaa
    17761 atccgtattt gaactgacac agcaacatca ccacgatcac ctgatttgcc tcgactgcgg
    17821 caaggttatc gaatttagtg atgattccat cgaagcgcgt cagcgtgaaa ttgccgcaaa
    17881 acatggcatt cgcctgacta accacagtct ctatctttac ggtcactgtg ccgaaggcga
    17941 ttgccgcgaa gatgagcacg cgcacgaagg caaataagcc agcctgaacg agaaaagcca
    18001 acctgcgggt tggctttttt atacaaggga aataagaaaa gcgagagtta cagctctcac
    18061 ttatttgtta ttactgcgta tttcctgcca gaccttatca caatcggctt tcaccgcctg
    18121 atcatttccg gtttgcgttg cacgcagaca ctgctgataa tccagtacgc gtacactttc
    18181 ctggtcggca aactgctgat gttgtttctc tttcttcagc acgtttaaca cgctctggca
    18241 ggcttcaatt ttttctggcg aaccttgtgc ggtgttgata caggcgctgt atgcctcttt
    18301 cagacgactg tcttcctgtg gcgcggttga ttgcgcacaa gctaccagcc ccgatgccat
    18361 tatggcgatg agaatcagtt ttttcatagc aaattcctca aacgtgcggc aggcacaaaa
    18421 cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac
    18481 gctcatcctg gaagatgtta ccgtaaccgc cgccccagct tgggatgtca gagtggttgt
    18541 cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt
    18601 agaccgcatc caggctgtag gcagactctt caatagtccg gtttttgtcg tagtacgcat
    18661 ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga
    18721 tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag aagaccgctt
    18781 tttcgccgtt ggcgttgaag tcggaacggt tatcccacca gatatccagg cggccgttgg
    18841 aggaagcgta ggttggagtc atacgttgca ggaagtatcc ctgctgaccg tcagccttaa
    18901 cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaatg
    18961 cttgcagcca agcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc
    19021 gggtaccgta gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc
    19081 tggctttggc aaaatattga tcgatatacc cttccgcctg accaaatgcc gcttccagta
    19141 cgaagttatt tttgaagtcg tatttcgccc caagggagtg cagataatca actttggtgg
    19201 ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggcgctttg tattcgttgg
    19261 tccacatgta ggagaaactc aaggcaccag catcgccgta atcaaaattc gccccggctt
    19321 ccgccccctg ataagtacct ggcataaagc tccagtgcgg cgctaacagc gtttgaccag
    19381 ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt
    19441 tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattacttt
    19501 ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt tcaatcgccg
    19561 taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga aaatcgaggt
    19621 tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt
    19681 tacggtcgcg ttcacgctgc cagtaatata taccgccggt taaggttgaa tcgtcgatga
    19741 atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcga
    19801 tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga
    19861 cgtataaatt gctgcaccaa aggtgaacag cgaaaaaata aaaattaaaa aagtagcgtt
    19921 gagaaaagtc tcgctgacta caggaacaga ctatttttcg cgacgcgaat tatcaatctc
    19981 tttctgtaac cagaatgtaa gaacatgagc aaccgcacat attttgatga tttttgttac
    20041 aaggcttaag tttgcggttg aaaggcttgc agaagaataa aagcgtgaat ttccagcaag
    20101 atgaaagcgg ttacatttta tttaattatg gtttgtgatt ttaaatttat tttatggata
    20161 aattgcgaag cggaattaat tcgcgaagaa aagcagaaaa aacgccgcag agtgcggcgt
    20221 ttgggattgt ttgctatttc ttgccggatg cgacgtaaat gcctcacccg gcctggtaga
    20281 acttaaaatt actcgcctac tttcgcccag gtatcacgca gcccaacggt gcggttaaat
    20341 accggttttt ccgctgtgga atggcggctg tcgaggcaga agtaaccttc acgctcaaac
    20401 tggaatgctt tacccgcaac cgcatctttc agcgacggtt cagcaaagcc ctgtttgatc
    20461 accagcgatt ccgggttaat caccgacagg aaatcatccg cagcaccagg gttcggcacg
    20521 ctgaacagac ggtcgtacag acggatttca accggcagcg catgtgccgc gctcacccag
    20581 tgaataacac ctttgacttt acgaccatct gccggatctt tgcttaaggt gtcggcgtca
    20641 taagtacaga agatggtggt gatattgcct tcggcatctt tctccacgcg ttctgcctta
    20701 atgacgtaag cattacgcag acgcacttct ttacccagca ccagacgttt gtactgcttg
    20761 ttagcttctt cgcggaaatc ggcgcgatcg atccaaatct caccgctaaa cggcacctgg
    20821 cggctaccca tttccggttt gttcggatgg ttcggcatgg taaccatttc gccttcgccc
    20881 tgatagtttt cgataaccag tttcaccgga tcgataaccg ccattgcgcg cggcgcattt
    20941 tcgttgagat cttcacggat gcaggattcc agcgatgcca tctcaatggt gttgtcctgc
    21001 ttggtcacgc cgatgcgttt gcagaactca cgaatagaag cagcagtgta accacgacga
    21061 cgcagaccgg aaatggtcgg catacgcggg tcatcccagc cttcaacgtg cttgtcggcc
    21121 accagcaggt tcagcttacg cttggacatc acagtgtatt ccagattcag gcgcgagaat
    21181 tcgtactggc gcgggtgaac aggaatggtg atgttgtcca gcacccagtc gtacaggcga
    21241 cggttgtcct ggaactcaag cgtacacaga gagtgcgtaa taccttccag ggcatcgctg
    21301 atgcagtggg tgaagtcgta catcgggtag atgcaccact tgttgccagt ctggtggtgt
    21361 tcagcaaatt taatacggta cagcaccgga tcgcgcatca cgataaacgg tgaagccatg
    21421 tcgattttcg cacgcaggca ggctttacct tcttcaaaac caccggcacg cattttttcg
    21481 aacagcgcca ggttctcttc aacgctgcgg tcgcggtacg ggctgttttt acccggttgc
    21541 gtcagggtgc cgcggtattc gcggatctgt tccggcgtca gttcatcaac gtacgccagg
    21601 cctttattga tcagttcgat cgcataggcg tggagctgat caaaataatc ggaggagtaa
    21661 cggacgttac cagaccagtg aaaacctaac cactctacgt cgtttttgat cgactcaaca
    21721 tactcgatat cttcttttac cgggttagtg tcgtcgaaac gcaggttgca ctggccttta
    21781 tagtcctggg cgatcccgaa gttcaggcag atagatttcg catggccaat atgcagatag
    21841 ccattcggct ccggcgggaa acgggtgtgt actgtggtgt gcttaccact ggccagatct
    21901 tcatcgatga tctgacggat aaagttagtc gggcgggctt ctgcctcact catcgtggat
    21961 tcctcaaagc gtaaacaacg tataacggcg tatgatctta taagcgggac aggctgacaa
    22021 ctgttagttt tttaaaattg ataatctaac aaggattatg gggatgattg atgaaaaaaa
    22081 aagcggcaga gaagatctcc accgcttgtc attgttggat gcgacgctca agcgtcgcat
    22141 caggcataaa gcagattact ttttgatttc atacagcggt gtttgacccg ccacaacatg
    22201 gccctgagct ttaatgatca agccactgaa atcgtcgata ttgctgcaaa ccaccgggct
    22261 aatcatcgag cgggcgttag cgttcaggta atccagatcc atttccagaa tcggttgccc
    22321 tgcgcttacc tgcgcaccct cttccaccag acgtttaaag cctttacctt ccagcgctac
    22381 ggtgtcgata cccatatgga cgacgatctc cgcgcctttt tcggtttcca ggcagaacgc
    22441 gtggttggtg ttgaagattt tcacgattgt ccctgcggct ggtgatacga cgattttatc
    22501 tgtcggtttc accgccacac cgtcacccac cgctttgctg gcgaatgctt cgtcaggaac
    22561 ctgatccagt gccacgacat caccggtaat cggcgatacc agctccgcga tagataccgc
    22621 gtttggtaca gcctgcggtt ttgctacagg cgcggcagtt gccggagttg cttcagctga
    22681 cgcagcggct accggaccac gggcaacgac tttcttcatc gcatcgccga tggattctgc
    22741 tttcgcgcca acaatcacct gaatagtctg tttgttcagt ttcactacac cagaagcacc
    22801 cagacgttta cacatcgtat cgttgacgcg ggcagagtca gccacggtca ggcgcagacg
    22861 ggtgatacag gcgtcaattg ctttcaggtt gtcagtgccg ccaaccgcag caatatagtt
    22921 ggtcgccagt tgattgaggc cttcttcagt gttgctgttg gcttcttcag taacgatctc
    22981 gtcttcttta tcttcacgac ccggcgtttt caggttgaac atgcggataa ccaaactgaa
    23041 caccacgaag tagatagcga agaagacaac acccataacc agcagcatcc agacgttctg
    23101 gctggcggcc ggcaggttat acatcaacgc gtagtcgata gcccccgcag agaaagagaa
    23161 gccagcatgg atacccagca gtgttgccac aaacaggctg ataccggtca gcagtgcgtg
    23221 caggaggtac agcagcggtg caaggaacat gaacaggaat tccagcggct cagtcacacc
    23281 ggtcaggaac gcagtaacag caacagaaag cagcatcccg ccaaccatcg gacgacgctc
    23341 tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc agaccgaaca tcataatcgg
    23401 gaagaagccg gacatgaaca tccccgcggt gccgtcaccg gcatagaagc ggttgatatc
    23461 accgtggaaa accgtacccg ccgcgttggt gaattcacca atctggaacc aggcgatggt
    23521 gttcagcacc tgatgcagac cggttgggat cagcagacgg ttgatgaaac caaagatacc
    23581 ggaacccagc gcgcccgcag aaacgatcca ctcgcctcct gcatggatag cgtgctgtac
    23641 cggcggccag acgtaaccaa aaatggccgc cagcaccagg cagaagaagc cggtggcaat
    23701 cggcacaaag cgtttgccgc cgaagaaact caggaagtcc ggcagtttaa tatcggacca
    23761 acggttatag gctgcgccac caaccagacc ggtaatgata cccgccagta cacccatgtt
    23821 aatttctggg ttgatggtca ccatcgcttt ggttaacaca aagtaaccta ccgcacccgc
    23881 cagcgccgcc gcacctgcgc tgtctttcga ccagctggat gccacaccga tggcgaagat
    23941 taatgcgagg ttatcaaaaa tcgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa
    24001 atctggctga ccgaatcgca gcaacagtgc cgccaccggc agcaccgcga tagggagctg
    24061 taacgcccta ccgagtcgct ggaaaaaacc taaaatattc atcttattcc ccctacgaga
    24121 accctatttg gctcgtttaa agccgtattt ttattttgct gcaaattgta ctgccgatgt
    24181 tctgtaatca gattgttaga tcatctgcta cagagtgtgt gaaaatttaa ttcgtatcgc
    24241 aaattaaacg cgtgtctttt gtgagttttg tcaccaaata tcgttatcat cactcccttt
    24301 tactggctaa agcagaaaac ttattttatc attcaaaaaa tcaggtcgga ttgacgcctg
    24361 tctgcgcaaa tccaggttac gcttaaagat gcctaatccg ccaacggctt acattttact
    24421 tattgaggtg aataatgaga ctgatccccc tgactaccgc tgaacaggtc ggcaaatggg
    24481 ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc gactgccgat cgtccgtttg
    24541 ttctgggcct gccgactggc ggcacgccga tgaccaccta taaagcgtta gtcgaaatgc
    24601 ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt caacatggac gaatatgtcg
    24661 gtctgccgaa agagcatccg gaaagctact acagctttat gcaccgtaat ttcttcgatc
    24721 acgttgatat tccagcagaa aacatcaacc ttctcaacgg caacgccccg gatatcgacg
    24781 ccgaatgccg ccagtatgaa gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg
    24841 gcggtgtagg taacgacggt catattgcat ttaacgaacc ggcgtcttct ctggcttctc
    24901 gtactcgtat caaaaccttg actcatgaca ctcgcgtcgc gaactctcgt ttctttgata
    24961 acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg tgttggtaca ttgctggatg
    25021 ccgaagaagt gatgattctg gtgctgggta gccagaaagc actggcactg caggctgcag
    25081 ttgaaggttg cgtgaaccac atgtggacca tcagctgtct gcaactgcat ccaaaagcga
    25141 tcatggtgtg cgatgaacct tccaccatgg agctgaaagt taagacttta agatatttca
    25201 atgaattaga agcagaaaat atcaaaggtc tgtaattgtt atccctgccc tggctccttg
    25261 ctcagggcaa tatttttaaa aatcgggggt cagaatgtat gcattaaccc agggccggat
    25321 ctttaccggc cacgaatttc ttgatgacca cgcggttgtt atcgctgatg gcctgattaa
    25381 aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa caacgttcac tgaacggggc
    25441 cattctctcc cccggtttta tcgatgtgca gttaaacggc tgcggcggcg tacagtttaa
    25501 cgacaccgct gaagcggtca gcgtggaaac gctggaaatc atgcagaaag ccaatgagaa
    25561 atcaggctgt actaactatc tgccgacgct tatcaccacc agcgatgagc tgatgaaaca
    25621 gggcgtgcgc gttatgcgcg agtacctggc aaaacatccg aatcaggcgt taggtctgca
    25681 tctggaaggt ccgtggctga atctggtaaa aaaaggcacc cataatccga attttgtgcg
    25741 taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac gccgacgtca ttaccaaagt
    25801 gaccctggca ccggaaatgg ttcctgcgga agtcatcagc aaactggcaa atgccgggat
    25861 tgtggtttct gccggtcact ccaacgcgac gttgaaagaa gcgaaagccg gtttccgcgc
    25921 ggggattacc tttgccaccc atctgtacaa cgcgatgccg tatattaccg gtcgtgaacc
    25981 gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat tgcggtatta tcgctgatgg
    26041 cctgcatgtt gattacgcca acattcgtaa cgctaaacgc cttaaaggcg acaaactgtg
    26101 tctggttacc gacgccaccg cgccagcagg tgccaacatt gaacagttca tttttgcggg
    26161 taaaacaata tactaccgta acggactttg tgtggatgag aacggtacgt taagcggttc
    26221 atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa cattgcggta tcgcactgga
    26281 tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg attggcgttg agaaacgtct
    26341 cggcacactc gccgcaggta aagtagccaa cctgaccgca ttcacacctg attttaaaat
    26401 caccaagacc atcgttaacg gtaacgaggt cgtaactcaa taagagaaag tatgacacca
    26461 ggcggacaag ctcagatagg taatgttgat ctcgtaaaac agcttaacag cgcggcggtt
    26521 tatcgcctga ttgaccagta cgggccaatc tcgcggattc agattgccga gcaaagccag
    26581 cttgcccccg ccagcgtaac caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa
    26641 gaagttgatc agcaggcctc caccgggggc cgccgcgcta tctccatcgt caccgaaacc
    26701 cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcacg acgccaccat caccctgttt
    26761 gatctcagca gcaaagtgct ggcagaagaa cattacccgc tgccggaacg tacccagcaa
    26821 acgctggaac atgccctgtt gaatgccatt gctcagttta ttgatagcta ccagcgcaaa
    26881 ctgcgcgagc tgatcgcgat ttcggtgatc ctgccagggc ttgttgaccc ggacagcggc
    26941 aaaattcatt acatgccgca tattcaggta gaaaactggg ggctggtaga agcactggaa
    27001 gaacgtttta aagtgacctg tttcgttggt cacgatatcc gtagtctggc gctggcggag
    27061 cactacttcg gtgcaagtca ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga
    27121 accggggccg ggattatctc taacgggcgc atttttattg gccgcaacgg caacgtcggt
    27181 gaaattggcc atattcaggt cgaaccgctg ggtgaacgct gccactgcgg caactttggc
    27241 tgcctggaaa ctatcgctgc caacgccgct attgaacaac gggtgttgaa tctgttaaag
    27301 cagggctacc agagccgcgt gccgctggac gactgcacca tcaaaactat ctgcaaagcc
    27361 gcgaacaaag gcgatagcct ggcctcggaa gtgattgagt atgtcggtcg tcatctgggc
    27421 aaaaccatcg ccattgctat caacttattt aatccgcaaa aaattgttat tgccggtgaa
    27481 atcaccgaag ccgataaagt gctgctccct gctattgaaa gctgcattaa tacccaggcg
    27541 ctgaaggcgt ttcgcactaa tctgccggtg gtacgttctg agctggatca ccgctcggca
    27601 atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg gtattttgct ccagcatttg
    27661 ctggaaaatt aatgtgcttt tatagtggcg cttattgttg tcaatattct gggtagtcca
    27721 tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct gatgcacgat aacgtcgccg
    27781 taccgggtgc agcggaattt ttgcacggga ttatggataa aggcctgccg ctggtgttgc
    27841 tgaccaacta tccttcgcag accgggcaag atctggcgaa ccgctttgcc actgccggtg
    27901 tcgatgtgcc ggatagcgtg ttttatacct ctgcgatggc gactgccgat ttcctgcgtc
    27961 gccaggaagg caagaaagcg tatgtggtgg gcgaaggcgc actgattcat gaactgtaca
    28021 aagccggttt cactattacc gatgtgaacc ctgattttgt gattgttggc gaaacgcgtt
    28081 cctacaactg ggacatgatg cataaagcgg cctatttcgt cgctaacggt gcacgcttta
    28141 tcgccaccaa cccggacacc cacgggcgcg gtttttatcc cgcttgtggc gcgttgtgtg
    28201 cagggattga gaaaatctcc gggcgcaaac cgttctatgt tggtaagccc agcccgtgga
    28261 tcatccgcgc ggcattaaac aaaatgcagg cgcattcgga agaaacggtg attgtcggcg
    28321 ataacttgcg taccgatatt ctggcgggct tccaggcggg tctggagacg attctggtgc
    28381 tttctggtgt ttcatcgctc gacgatatcg acagtatgcc gttccgccca agctggattt
    28441 acccgtcggt cgctgaaatc gacgttatct gaatagcaac cacgtctcag ggcgtggttt
    28501 cttattttta cccatcatta aaatcatccc attcaataaa actcgccaaa aattatcgat
    28561 ccatcaataa atacatcacc acaccatccc ctttttcaca aatcagcaat caccattacg
    28621 ttttttattt ttgcactgcc aaatcgcttg cgcacagtgc tgctttgcgg cattttttta
    28681 aacaagcaaa cacaacaagc aacaaatacc aggttaacgg agaaggttat gtgttcaatt
    28741 tttggcgtat tcgatatcaa aacagacgca gttgagctgc gtaagaaagc actcgagctg
    28801 tcacgcctga tgcgtcatcg tggcccggac tggtccggta tttatgccag cgataacgcc
    28861 attctcgccc acgaacgtct gtcaattgtt gacgttaacg cgggggcgca acctctctac
    28921 aaccaacaaa aaactcatgt gctggcggta aacggtgaaa tctacaacca ccaggcactg
    28981 cgcgccgaat atggcgatcg ttatcagttc cagaccggat ctgactgcga agtgatcctc
    29041 gcgctgtatc aggaaaaagg gccggaattt ctcgacgact tgcagggcat gtttgccttt
    29101 gccctgtacg acagcgaaaa agatgcatat ctgattggtc gcgaccatct ggggatcatc
    29161 ccactgtata tgggctatga cgagcacggt cagctgtatg tggcctcaga aatgaaagcc
    29221 ctggtgccag tttgccgcac gattaaagag ttcccggcgg ggagctattt gtggagtcag
    29281 gacggcgaaa tccgttctta ttatcatcgc gactggttcg actttgatgc agtgaaagat
    29341 aacgtgaccg acaaaaacga gctgcgtcag gcactggaag attccgttaa aagccatctg
    29401 atgtctgacg ttccttacgg tgtgctgctc tcgggtggtc tggattcctc aattatttcc
    29461 gcgatcacca agaaatacgc cgcccgtcgc gtggaagatc aggaacgctc tgaagcctgg
    29521 tggccgcaat tacactcctt tgctgtaggt ctgccgggtt caccggatct gaaagcagcc
    29581 caggaagtgg caaaccatct gggcacggtg catcacgaaa ttcacttcac tgtacaggaa
    29641 ggtctggatg ccatccgcga cgtgatttac cacatcgaaa cctatgatgt gacaactatt
    29701 cgcgcttcaa caccgatgta tttaatgtcg cgtaagatca aggcaatggg cattaaaatg
    29761 gtgctgtccg gtgaaggttc tgatgaagtg tttggcggtt atctttactt ccacaaagca
    29821 ccgaatgcca aagaactgca tgaagagacg gtgcgtaaac tgctggccct gcatatgtat
    29881 gactgcgcgc gcgccaacaa agcgatgtca gcctggggcg tggaagcacg cgttccgttc
    29941 ctcgacaaaa aattccttga tgtggcgatg cgcattaacc cgcaggataa aatgtgcggt
    30001 aacggcaaaa tggaaaaaca catcctgcgt gaatgttttg agtcatacct gcccgcaagc
    30061 gtggcctggc ggcagaaaga gcagttctcc gatggcgtcg gttacagttg gatcgacacc
    30121 ctgaaagaag tggcggcgca gcaggtttct gatcagcaac tggaaactgc ccgcttccgc
    30181 ttcccgtaca acacgccaac ctcaaaagaa gcgtatctgt accgggagat ctttgaagaa
    30241 ctgttcccgc ttccgagcgc cgctgagtgc gtgcctggcg gtccttccgt cgcgtgttct
    30301 tccgctaaag cgattgagtg ggatgaggcg ttcaagaaaa tggacgatcc gtctggtcgt
    30361 gcggttggcg ttcaccagtc ggcgtataag taagaaatgc gaaacagccc cggtgaaatc
    30421 cggggctgtt tcagttattg aggtgcttgt taattctgat atgcagtaag gaaatgttcc
    30481 gggtttgcca catcactgtt tgacaactca tcactgtagg ccagtatttc atagattaat
    30541 ttcccaccac tattgttgaa ttccgaattt catcctgtac gacaaaagaa tggctgtagg
    30601 gtggggaaaa aacacaaagt aaacaataat tgacgaatat agcgccacgc tgttcgcaac
    30661 ctaaccaaac agtcactttc gagcaatttt ccttgaaaaa gaggttgacg ctgcaaggct
    30721 ctatacgcat aatgcgcccc gcaacgccga taaggtatcg cgaaaaaaaa gatggctacg
    30781 tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc
    30841 gtagccacca tcttttttgc gggagtggcg aaattggtag acgcaccaga tttaggttct
    30901 ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca ccattcacca gaaagcgttg
    30961 tacggatggg gtatcgccaa gcggtaaggc accggttttt gataccggca ttccctggtt
    31021 cgaatccagg taccccagcc atcttcttcg agtaagcggt tcaccgcccg gttattgggg
    31081 tatcgccaag cggtaaggca ccggtttttg ataccggcat tccctggttc gaatccaggt
    31141 accccagcca tcgaagaaac aatctggcta cgtagctcag ttggttagag cacatcactc
    31201 ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac caaattctga atgtgtcgaa
    31261 tacgttcggc aaattcaaaa accaatttgt tggggtatcg ccaagcggta aggcaccgga
    31321 ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccaattta ttcaagacgc
    31381 ttacatcgta agtgcgccca gttggggtat cgccaagcgg taaggcaccg gattctgatt
    31441 ccggcattcc gaggttcgaa tcctcgtacc ccagccacat taaaaaagct cgcttcggcg
    31501 agctttttgc ttttctgcgt tcattcaatg tcgaatgcga tgttgacacg tcttatcctt
    31561 caatgtcgga tgcgacgctg ccgcgtctta tccgacctac ggttggcacg catccggcaa
    31621 tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg acgtttcaac acgccagcac
    31681 gctctgccgc cattaacccg agattacgca caaaacgcag cggcggcaga ttattactga
    31741 atccggcata aaacagatcc ataccacttt gcataatgaa gttatccgcc atgcgccgca
    31801 tctggtaacg cttgaggaca ggataactgg cccacgcttc accgtagctg cgggcattca
    31861 ccagaacatc aatcagggca tcgacatcac gataaccaag attcaccccc tgccccgcca
    31921 gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag ccccggctgc acatactgca
    31981 acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag cggtgtaacg taacccagac
    32041 gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc catattcata ttctgcaact
    32101 ggcgaatacg taccggagag tcataccaca ccagcgatgc ccagttatca aacaacggca
    32161 gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt gctgtcgccg ggatcgttct
    32221 cacactgtac gctaatcaac atacacgact gcgcatactg ccaggcatga acgccaattc
    32281 ccgccatctg ccgcacctgc gaatttgcgc cgtcagcacc aatcaccagc ttcgcgcgaa
    32341 tcacttcacc gcccttcagt tccagctcct gaagatcatc atggcgatgc agcgcaatca
    32401 gtgagcctgg cacacgtaac gttactttcg gatgcgcttc cagcgcctgc cacagcgcct
    32461 gttgcaggac ggtgttttcc accatatagc caagcagcgg cagcttaagt tcagcggcgt
    32521 caaacatcac atgcgccgtt tcccactccc acgtttccag tctgcggtaa ggatggcaac
    32581 gcatagcctg tactgcatcc cagaccccta accctttaag caatgatacc gatgcggcgc
    32641 tgatcgccga gatccgcacg tccggttggc tatcagcgac aaacggtgca ggttctgcgt
    32701 gctcgatcac cgttaccgaa aatccgtgct gtgccagccc cagcgccagt gcgccgccga
    32761 ccattcctcc gccgacaatg gcaatttccg ttggttgatt tgtcatggtc aatcatcctg
    32821 ttagcaatat gctttaagtt tacaggattt ttacccccaa gggctgataa cgctcatact
    32881 ggtcacaacg acagcaaagc attacactat gcgccctgca ttcctggcta ctatttcgca
    32941 agagcaagtc gatgaccaaa aaactccata ttaaaacctg gggctgtcag atgaacgagt
    33001 acgattcatc gaagatggcc gatctgctgg atgccaccca cggctatcaa ctgaccgacg
    33061 tggcggaaga agcggatgtg ctgctgctga acacctgctc aatccgcgag aaggctcagg
    33121 aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa agagaagaat ccagacctga
    33181 ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga gcacattcgc cagcgcgccc
    33241 actatgtcga tattattttt gggccgcaaa cgctgcaccg tctgccggag atgatcaact
    33301 ccgtgcgtgg cgaccgcagc ccggttgtag atatcagctt cccggaaatc gagaagtttg
    33361 accgtctacc ggaaccgcgc gccgaagggc cgaccgcgtt tgtctccatc atggaaggct
    33421 gcaataaata ttgcacctac tgcgtggtgc cttacacccg tggtgaagag gtaagccgtc
    33481 cgtccgacga tattctgttt gagattgccc agcttgcggc tcagggcgtg cgtgaagtca
    33541 acctgctcgg tcagaacgtg aacgcctggc gtggtgaaaa ctacgacggc accaccggat
    33601 cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg gatcgatcgt attcgcttta
    33661 ccaccagcca tccgatcgaa ttcaccgacg atatcatcga agtgtaccgc gacacgccgg
    33721 agctggtgag cttcctgcac ctgccggtac agagtggttc cgatcgcatt ctgaacctga
    33781 tgggccgtac ccatacggcg ctggagtaca aagcgatcat ccgtaaactg cgtgcggcgc
    33841 gcccggatat tcagatcagt tctgacttca tcgtcggctt ccctggcgaa accaccgaag
    33901 acttcgagaa aacgatgaag ctgattgccg acgtcaattt cgacatgagc tacagcttta
    33961 tcttctctgc acgtccgggg acgcccgccg ccgatatggt ggatgatgtt ccggaagaag
    34021 agaagaagca gcgtctgtat attctgcaag aacgtatcaa tcagcaagcg atggcgtgga
    34081 gccgccggat gctcggcacc acccagcgta ttctggtaga aggtacatcg cgtaagagca
    34141 tcatggagct ttccgggcgt acggaaaata accgcgtggt caacttcgaa ggcacgccgg
    34201 atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt ctacccgaac tctctgcgcg
    34261 gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat ggcagaaaca ccggagtcgg
    34321 tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg ttattatcag ccgtaattct
    34381 caggcctgcc gttccggtgg gccttgtttt ttctctcttt atccccatct ttatcgcaat
    34441 gcttgcgcct ttttcctgtg gcgtgaataa tttccttata ggccggtgaa tgttcagctt
    34501 ccggcggcat acagataaga ggaacggttt gaacatagac actcgcgaaa tcaccctgga
    34561 gccagcagac aacgcgcgtc tgttgagcct gtgcggcccg tttgatgaca acatcaagca
    34621 gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat aaccacttta aactgaccgg
    34681 ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt agcctgtatg tcgatactgc
    34741 cccgatgcgc ggtcagattc aggatatcga accggaacag atccaccttg cgattaaaga
    34801 agcaagggta ctggagcaaa gcgcggagag cgtgccggag tacggcaaag cggtcaatat
    34861 caaaaccaaa cgcggcgtaa ttaagccgcg cacgccaaac caggcgcagt acatcgccaa
    34921 tattctcgac catgacatta ccttcggcgt tggcccggcg ggtacgggta aaacctacct
    34981 ggcagtcgct gcagcagttg atgccctgga gcgtcaggaa attcgccgta ttctgctgac
    35041 tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg cctggcgatt taagccagaa
    35101 agtagacccg tatctgcgcc cactgtacga cgcgctgttt gaaatgctgg gctttgagaa
    35161 agttgagaaa ctgattgagc gcaacgttat tgaagtcgcg ccgctggcct atatgcgtgg
    35221 tcgtacgctg aacgacgcgt ttatcattct cgatgagagc cagaacacca ccatcgaaca
    35281 gatgaagatg ttcctgaccc gtatcggttt taactcaaaa gcggttatca ccggcgacgt
    35341 cacacagatc gacctgccgc gtaatactaa atcaggctta cgtcacgcca tcgaagtgct
    35401 ggccgatgtc gaagagatca gctttaactt cttccacagc gaagacgtgg ttcgtcaccc
    35461 tgtggtggcg cgtatcgtta acgcctatga agcctgggaa gaagccgaac aaaaacgaaa
    35521 agcggcgctg gcggcagaac gcaagcgcga agaacaggaa caaaaatgag tcaggtgatc
    35581 ctcgatttac aactggcatg tgaagataat tccgggttac cggaagagag ccagtttcaa
    35641 acatggctga atgcggtgat cccgcagttt caggaagaat cggaagtgac tattcgcgtg
    35701 gtcgataccg ccgaaagcca cagtctgaat ctgacctatc gcggtaagga taagccgacc
    35761 aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg aaatgtcgct actgggcgat
    35821 ctggttatct gccgtcaggt ggttgagaag gaagctcagg agcaaggcaa accactggag
    35881 gcgcactggg cgcatatggt ggtgcacggc agtctgcatt tgttaggtta cgatcacatc
    35941 gaagatgacg aagcagaaga aatggaagcc ctcgaaacag agattatgct tgctctgggc
    36001 tatgaggatc cgtacattgc cgagaaagaa taatttgcca ggcatttgac tggcagacgt
    36061 ccccgccgtt ggtgattaac gacggcgttt tgattaacta acatgagaac ccatacgacg
    36121 ccatgagcga cgacaattca cacagtagtg acacgataag caacaagaag ggatttttct
    36181 ccctgttact cagccaactt ttccacggtg aaccgaaaaa ccgtgacgaa ctgctggcgc
    36241 tgatccgtga ttccgggcag aacgacctta tcgacgaaga tacgcgcgat atgctcgaag
    36301 gggtgatgga catcgcagac caacgcgttc gcgacatcat gatcccccgc tcccagatga
    36361 ttaccctgaa acgcaaccag acgctggacg aatgccttga tgtcatcatc gagtccgccc
    36421 actcacgttt cccggtgatt agcgaagaca aagatcacat tgaagggatt ctgatggcga
    36481 aagacttgct gccgtttatg cgcagcgatg ctgaagcctt cagcatggac aaagtgttac
    36541 gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg gatgctgaaa gagtttcgct
    36601 ctcagcgtta ccacatggcg atcgttattg acgaattcgg tggggtttcc ggtctggtga
    36661 ccattgaaga catcctggaa ctaattgttg gtgagattga agacgagtat gacgaagaag
    36721 atgatatcga cttccgtcag ctgagtcgtc atacctggac cgtgcgcgca ctggcttcca
    36781 ttgaagactt caacgaagcg ttcggcaccc actttagcga tgaagaggtt gacactatcg
    36841 gtggtctggt gatgcaggca tttgggcatc ttccggcgcg tggcgaaact atcgacatcg
    36901 acggttacca gttcaaagtg gcgatggccg acagtcggcg tattattcag gttcatgtca
    36961 aaatcccgga tgactcaccc cagccgaagc tggatgaata aaaccgaaac tggatagata
    37021 actacatggc ttttgcctca ttaattgaac gccagcgcat tcgcctgctg ctggcgttat
    37081 tattcggtgc ctgcggaacg ctggccttct ctccttacga tgtctggcct gcggcgatta
    37141 tttcgctgat ggggcttcag gcgttgacct ttaaccgccg tccactccag tctgccgcta
    37201 ttggcttttg ctggggattt ggcctctttg gcagcggtat taactgggtc tatgtcagca
    37261 tcgcgacctt tggcgggatg cctggcccgg ttaacatctt cctggtggtg ctgctggcgg
    37321 cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct gtcgcgtctg tggccgaaaa
    37381 ccacctggct gcgcgtggcg attgccgccc cttctctctg gcaagtgacc gagtttctgc
    37441 gcggttgggt actgacaggc ttcccgtggt tacagttcgg ctatagccag attgatggcc
    37501 cgttaaaagg gctggcaccg ataatgggcg tggaagccat taacttcctg ctgatgatgg
    37561 ttagtggcct gctggcactg gtgttggtca aacgcaactg gcgtccgctg gtggtggccg
    37621 tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca atggtttacc ccacaaccgg
    37681 agaaaaccat tcaggtttcg atggttcagg gcgatattcc gcaatcactg aaatgggatg
    37741 gagatcagct tcttaatacg ctgaagattt actacaacgc aacggctccg ctgatgggca
    37801 aatcatcgtt gattatctgg ccggagtcgg cgataaccga tctggaaatt aatcagcaac
    37861 cgttcctcaa agcactggac ggtgagttgc gcgataaagg tagctcgctg gtaaccggga
    37921 ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac ctacaacacc atcatcacac
    37981 tgggtaaagg tgcaccgtac agctacgaat cagccgatcg ctataacaaa aaccatctgg
    38041 tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg tccgttagca ccgttctttg
    38101 atctgccgat gtcgtcgttc agccgtgggc catatatcca gccaccgctg tcggcaaatg
    38161 gtattgagct tactgcggct atttgctacg agatcattct cggcgagcaa gtgcgcgata
    38221 acttccgccc ggataccgac tatctgctga ctatctccaa cgatgcgtgg tttggtaagt
    38281 ctattggtcc atggcagcac ttccagatgg cgcgaatgcg tgcgctggag ctggcgcgcc
    38341 cactgttgcg cagcaccaac aacggcatta cggcggtgat tggcccgcag ggtgagattc
    38401 aggcgatgat cccgcagttc acccgcgagg tgttgaccac taatgtgaca ccgaccaccg
    38461 gactcacacc gtatgcacgt accggcaact ggccgctgtg ggtgctgacg gcgctgtttg
    38521 gttttgccgc tgtgctgatg agtctgcgag cacgtaaacg ttgatgtgcc tgatgtatcc
    38581 tgcatgagca aaacgtactt tctcattaag ctttggggca tggttatcat gccccttttt
    38641 cttacggttc agggatacaa gccacattgc catattgggg atgacgaata attgcctgcg
    38701 gatcaagcga gcgcgtcctg tccagttgga tcaacccgtt cttcgccata acaaatattt
    38761 ttctaccatc cgctgaaaaa cgaaggtgat taacgtagct ggcaggataa acgccaataa
    38821 tctgcccggc attaatattg agcaggatga cataaccttg caggccatat gacaccaacg
    38881 ctaaatactg accgtccggg ctaataccct caacggaata aacgctcctg ctacaccatt
    38941 gcgtcataat gctggcttgt tgatccccta cgcttatacg tgaaatcccc tggctgtcgg
    39001 caaaaattac atccccgttt tcaggtcgaa aacgcatcgc cgatatcgtt cctcgcgcat
    39061 cactagccgg atagctttct ttctgatgcg tgatggtatt gatgcgtaca atgtcacctc
    39121 tgaccatacc gggtatcaac aatatttttt catccgggga aagagccata acccaggcac
    39181 caggtttatt actcgtcccc gtcccgtcgt atttctccag cgggatatcc gccacatgct
    39241 gataagtcac cagatcaatc gctgacacac tattactggt gcgattcatc acccaggctt
    39301 ttgtaccatc acgcgagagg ataaggctgt caggatgtgc accggtggta atcgattgaa
    39361 taatctcccc tgtcgccacg ttaatgaccg ataacgcatt cgcttcaaat gaagtcgcat
    39421 aaatcaactt ttcatccggg cttaatgcca gcgatctgac gccctgatct ttacagccac
    39481 cacaatgttt gactgacttt tctttcgcta tgggcgctgt aaaggtatgc aggattttct
    39541 tttgtccgag gtcgaaaaca acaatgctgt tcgtgttttc aattgcggca tacaacatat
    39601 taccatcccg actgatcgcc atatcatgga tatcggcaat ctcagcgagc aaatcaccac
    39661 tggcctgttc acgacttacc ggagcggata atgtttccct gagggcgttg cgttcctccg
    39721 ctaactgctg ctttttttgc cgtcgctctt gttcaatctc ctgtggataa aacgcttcac
    39781 gcaggctttg cctgacttgt aaaactgact catccatctg cagctgcgca agccagatag
    39841 ccccggtcag aagaagactg cccgcaacaa taatcccgcc ttccgtaacg agtcggtttt
    39901 gcccagataa acgcgcgctc agccaaccca acgcgccacc cgcaatcagg ccgccaatgt
    39961 ggcaagcatt atctattcct gcttgccggg caccatacaa cagtgtcagc gccaccatac
    40021 ccaacagaga aaatactcgc cgctggtttt tatggtatgt gcccgcacct gaagcaagct
    40081 gtgttgctaa cgcagcgcca gctattccca taattgcccc ggacgcccca acgctgatga
    40141 ctatctgatc actatcgcgt aacgcataac aggcgctgat gagcccacca aagacgccgg
    40201 agaataacca aataatcaga agtcgccatt ttcccagtat ccgttccgcc actgccccca
    40261 ccaccaggag tgcaaacata ttcatcagca aatgggaaaa actactgtgc agaaagacac
    40321 tgctaaataa gcgccaggat tcacccgaca acgtgagaga agcaacgtta gcaccgagat
    40381 ggatgagtgc ggattgatcg acttcttcat aactcatcca ttgtgcgagg taacaaaata
    40441 tcccaacaca gagcgcaaaa aggataaggg ttattgcggg taattgaaca tttaacggct
    40501 taaccgaaga tgcagacata aggtgtttct tccttgaaaa gacaggtaaa aagcattttt
    40561 cccgtgtgca gttatcatcc cggcaaacgt cccatgccga aaagataatt ctgtccgaaa
    40621 tatgactttt tcagcctaaa attacaatcg taattaattc gcccctaagt attttttggc
    40681 atgtaagttg ctttatttac tgtgtaagcg atgaaatcag ccgcgacgca accaggttgc
    40741 accaccacaa atcagcggta gcaccaaaaa ggtgcggttt aagaattttg cttcggaatg
    40801 gtgcggcgca tctcgcaaaa acaaacaata aaacagcaat atctttacat ttagccaaac
    40861 taaatgttac ctaacaggca caacactgca caataaagtt gcagacgata acaacacaaa
    40921 cactcacaac gggtatccat gcgttcttaa cgcagaagat aaaggagttg gatatgcaat
    40981 tacgtaaacc tgccacagca atcctcgccc tggcgctttc cgcaggactg gcacaggcag
    41041 atgacgccgc cccggtagcg ggcagtacgc tggacaaaat cgccaaaaac ggtgtgattg
    41101 tcgtcggtca tcgtgaatct tcagtacctt tctcttatta cgacaatcag caaaaagtgg
    41161 tgggttactc gcaggattac tccaacgcca ttgttgaagc agtgaaaaag aaactcaaca
    41221 aaccggactt gcaggtaaaa ctgattccga ttacctcaca aaaccgtatt ccgctgctgc
    41281 aaaacggcac ttttgatttt gaatgtggtt ctaccaccaa caacgtcgaa cgccaaaaac
    41341 aggcggcttt ctctgacact attttcgtgg tcggtacgcg cctgctgacc aaaaagggcg
    41401 gcgatatcaa agattttgcc gacctgaaag gcaaagccgt agtcgtcact tccggcacta
    41461 cctctgaagt tttgctcaac aaactgaatg aagagcaaaa aatgaatatg cgcatcatca
    41521 gcgccaaaga tcacggtgac tctttccgca ccctggaaag cggtcgtgcc gttgccttta
    41581 tgatggatga cgctctgctg gccggtgaac gtgcgaaagc gaagaaacca gacaactggg
    41641 atatcgtcgg caagccgcag tctcaggaag cctacggttg catgttgcgt aaagatgatc
    41701 cgcagttcaa aaagctgatg gatgacacta tcgctcaggt gcagacttcc ggtgaagcgg
    41761 aaaaatggtt tgataaatgg ttcaaaaatc caattccgcc gaaaaacctg aacatgaatt
    41821 tcgaactgtc agacgaaatg aaagcactgt tcaaagaacc gaatgacaaa gcactgaact
    41881 aattacaaga accaggggcg gaaattccag ccctctcgat tgttacgtag cacggacaga
    41941 ctatacgcct gatggtcgtt ccccatcggg cctgaaaacc gcaatacgct gggtaacaat
    42001 cttcgagggt agcagttaac gctgctaccc tcttttttct ggagtagatt tatgtctata
    42061 gactggaact ggggtatttt tttacaacaa gccccgttcg gcaacaccac ctatcttggt
    42121 tggatctgga gtggttttca ggtgacgatc gctttgtcga tctgcgcctg gattatcgct
    42181 ttcctcgtcg gttcattttt cggcatttta cgtaccgttc caaaccgttt tctctccggt
    42241 ctgggtacgt tgtatgtcga actgttccgc aacgtgccgt tgattgtgca attctttacc
    42301 tggtatctgg tgatcccgga gctgttgccg gagaaaatcg gcatgtggtt taaggccgag
    42361 ctggatccca atattcagtt tttcctttca tccatgctct gcctggggct gtttaccgcc
    42421 gcccgtgttt gcgaacaggt acgcgccgcc attcagtcgt tgccgcgtgg gcaaaaaaat
    42481 gccgcgctgg cgatgggcct gacgctaccg caggcttatc gatacgtact gctgcctaat
    42541 gcttatcgcg ttatcgtccc gccgatgact tccgagatga tgaacctggt gaaaaactcc
    42601 gccatcgcct cgactattgg tctggtggat atggcggcgc aggcgggtaa attgcttgat
    42661 tactcagccc acgcctggga gtcgtttacc gctattacgc tggcgtatgt gttgattaac
    42721 gcggttatca tgctggtaat gacgctggtt gaacgtaaag ttcgcctgcc tggcaacatg
    42781 gggggcaaat aatgtacgag tttgactgga gttccattgt cccttccctg ccatatctgc
    42841 tcgacgggct ggtgatcacg ctgaaaatca ccgtcacggc agtcgtgatt ggtattttgt
    42901 ggggcaccat gctggcggtg atgcgcttgt ccagttttgc gcccgtagcg tggtttgcca
    42961 aagcctacgt taacgtattc cgctcgattc ctttagttat ggttttgctg tggttttacc
    43021 tgatcgtgcc aggttttctg caaaacgtgc tgggattatc accaaaaaat gatatccgtt
    43081 taatttcggc gatggtggcg ttttcgatgt ttgaagcggc ctactattca gagattatcc
    43141 gtgctggtat tcaaagtatt tctcgtggtc agtcgagcgc cgcgctggcg ttgggaatga
    43201 ctcactggca gtcgatgaaa ctgattattc tgccgcaggc gttccgcgcg atggtgccgc
    43261 ttctgctcac tcagggcatc gtactgttcc aggatacctc actggtgtat gtgttatctc
    43321 tggccgattt cttccgtacc gcctcaacca ttggtgagcg tgatggtacg caggttgaga
    43381 tgatcctgtt tgccggattt gtttatttcg ttattagtct tagcgcgtcg ttgttggtca
    43441 gctacttgaa aagaaggaca gcataatgat taccctgaaa aatgtttcaa aatggtatgg
    43501 tcactttcag gtgctgaccg actgctcaac cgaagtgaaa aaaggcgaag tggtggtggt
    43561 ttgcggcccg tctggttccg gcaaatcaac gctgattaaa accgtcaacg gcctcgaacc
    43621 agtgcagcaa ggtgaaatca ccgtcgatgg tatcgtggtt aatgacaaga aaaccgatct
    43681 ggcaaagctg cgttcccgcg tcgggatggt attccagcat ttcgagctgt tccctcatct
    43741 gtcgattatc gaaaacctga ccctggcgca ggtgaaagtg cttaaacgcg ataaagcgcc
    43801 cgctcgtgaa aaagcattga aattgctgga gcgcgtggga ctttccgcac atgccaataa
    43861 atttccggcg cagctttccg gcggtcagca gcagcgtgtg gcaatcgctc gcgcgttgtg
    43921 tatggatcct attgcgatgc tgttcgacga accgacatcg gcgctggatc cggagatgat
    43981 caacgaagtg ctggacgtga tggtggaact ggcaaacgaa ggaatgacca tgatggtggt
    44041 gacccacgaa atgggttttg cccgtaaagt ggcgaatcgg gtgatcttta tggacgaggg
    44101 taaaattgtc gaagactcgc cgaaagacgc tttcttcgat gatccgaaat cggaccgcgc
    44161 gaaagacttc ctcgcgaaaa tcctgcatta atcactctgg cgcgcatacc caagtgcgcg
    44221 ccatctcacg ctttatcgtt tcgcccctcg tatctgttac atcctggcgt tacccttgtc
    44281 gcaaagaagc acacaacaag gagcaacaat ggcactgcca attctgttag attgcgaccc
    44341 aggtcatgac gacgctatcg caatagttct cgccctcgcc tcaccagagc ttgatgtcaa
    44401 agcaattacg tcttccgccg gaaaccagac accagaaaaa accttacgca atgttcttcg
    44461 tatgctgacc ttgcttaatc gcaccgatat tccggtagca ggcggcgcgg taaaaccgtt
    44521 aatgcgtgag ttgattatcg cggacaatgt gcacggcgaa agcggtctgg atggcccggc
    44581 attaccggaa ccgacgttcg caccgcaaaa ctgtacggcg gtagagctga tggcgaaaac
    44641 gctgcgcgaa agtgcggaac ctgtcacgat tgtgtctacc ggaccgcaaa ctaacgttgc
    44701 cttgctgctc aatagccatc cggaactgca tagcaaaatt gcccgtatcg tgattatggg
    44761 cggcgcaatg gggcttggta actggacgcc tgcggctgaa tttaatattt acgttgaccc
    44821 ggaagcggca gaaattgtct tccagtcagg gatcccagtg gtgatggccg gtctggatgt
    44881 tactcacaaa gcacaaatcc acgttgaaga caccgagcgt ttccgcgcga ttggcaaccc
    44941 tgtttcaacc attgttgccg aactgctgga tttcttcctc gaatatcata aagacgaaaa
    45001 atggggcttt gtcggtgcac cgctgcatga cccatgcacc atcgcctggc tgttgaaacc
    45061 cgagttgttt accactgttg agcgctgggt tggcgtggaa acacagggga aatatagcca
    45121 gggtatgacg gttgttgatt attattatct gactggcaat aaaccgaatg ccactgtaat
    45181 ggtcgatgtt gatcgtcagg gctttgttga tttactggct gatcgtctga aattttacgc
    45241 ttaacacacg tcattgatta agatcaccac gcgttacctg ggtggtctta atctgtttat
    45301 ctctttttct ttcaagatat aaatgcaatg gataatgcag aactcgccat tggtatcgat
    45361 ctcggcacta ccaatagttt aattgccgtc tggaaagacg gtgccgcgca attaattcca
    45421 aataagttcg gtgaatattt aacaccatcc ataattagca tggatgaaaa taatcatatt
    45481 ttagtcggaa aaccggctgt atcacggcgt acttcgcatc cggataaaac ggcagcgtta
    45541 tttaaacgtg caatgggcag taataccaac tggcggttag gcagcgacac atttaacgcg
    45601 ccagaactgt cctctttggt attacgctca ttaaaagaag atgccgaaga atttctgcaa
    45661 cgtccgatta aagatgtggt gatctccgtt ccggcttatt tcagcgatga acaacgcaag
    45721 catacccgtt tagcagcgga gttagccggg ttaaatgcgg tacgcttaat taatgaaccc
    45781 acagcagctg cgatggcata tggcctgcat acgcaacaaa atacccgctc gctggttttt
    45841 gatctcggtg gcggcacgtt tgacgtcacg gtgctcgaat acgccacgcc ggtgattgaa
    45901 gttcacgcct ccgctggcga taacttcctc ggtggcgaag attttaccca tatgttggtc
    45961 gatgacgttt taaagcgcgc ggatgtcgcc aggaccacgc ttaacgagag tgaactggca
    46021 gccttgtacg cctgtgtgga agcggcaaaa tgtagcaatc aatcgccatt gcacattcgc
    46081 tggcagtacc aagatgaaac gcgggagtgc gaattttacg agaacgaact ggaagatttg
    46141 tggttgccgc tgctcaatcg cttgcgagtg ccgattgaac aggcgttgcg cgatgcacgt
    46201 ctgaagccga gtcaaatcga cagtctggtg ctggttggcg gcgcgtcaca aatgccgctg
    46261 gtgcagcgaa tcgccgtgcg tctgtttggc aaattaccgt atcaaagtta cgacccgagc
    46321 accattgtcg cgctgggcgc agcaatccag gccgcctgcc gcttacgcag tgaagatatt
    46381 gaagaggtga tcctcactga tatttgccct tactcgttgg gcgttgaagt taaccgccag
    46441 ggcgtttccg gcattttctc gccaattatt gaacgaaaca ccacagtacc cgtgtcgcgt
    46501 gtagaaactt actcgaccat gcacccggaa caggattcaa ttacggttaa cgtctatcag
    46561 ggagaaaacc acaaagttaa aaataacatt ctggttgaat ccttcgatgt gccgttgaag
    46621 aaaaccgggg cttatcagtc gattgatatt cgctttagtt atgatatcaa cggcttgctt
    46681 gaagttgacg tgcttctgga agatggcagc gttaagtcca gagtcattaa tcatagcccg
    46741 gtaacattga gcgcgcagca gattgaagag agtcggacgc ggttatccgc attgaaaatt
    46801 tatccgcgcg atatgctcat caaccgcacc tttaaggcca aactggaaga gttgtgggcg
    46861 cgggcgctgg gtgacgagcg agaagagatt ggccgggtga tcaccgattt tgatgcggcg
    46921 ttgcagtcaa acgatatggc ccgcgtcgat gaggtcaggc ggcgggcgag cgtttattta
    46981 gccattgaga cctcataatc acggctgccg gatgcggcgt aaatgctgca tccggcatcg
    47041 gcgcaaaaaa cacatcgtca actaaacaac atcgcgattt tgccgagagc ataacctata
    47101 accagcaccg ttaaactgat tttgataatg ctggtaatgc tctgtggttt ttcaccctca
    47161 tcattttttt cttcatggtt ttcaggtgtt ggctcatcga catcttcatc ctccggctcg
    47221 agttttactt tcgtcaacag caaccgggtg ataatggcct ctaatggatg agcatctccg
    47281 ccttccagcc ttgtattaag acgggccagc atctcttgtt tcggaaacgc ttcaagtact
    47341 acctgttccg ctacccgata caaatccagt aacggtgaat cttcttctgt ctcgctaccg
    47401 actctcgatt tccaccacgc ccatgctttg cgtttgaaga ataacgactc aataaacgat
    47461 tgacctaacg gcgataattt tccgctatgt aaaatttccg cccataacag ccgcgtccgg
    47521 gcgctctcac caggaacata tttcagcggt tgaccttgcg catctaccat gctccgcgct
    47581 tcgataacca tcactaataa cgcgaaataa tccgggcaat gacgcttgct ccactgcaac
    47641 aacaaattat cagcttgtgt agatgggtaa ctctcccagt atgcgcagag ctcagcaagc
    47701 aggctttccc cctcgccgca gtacaaacgt tgtgcacact ggtaataccg ggcatcttcg
    47761 ctttctggtt cttccgcttg ccactgtttg gctacaggaa tgagttccgc catacctgtt
    47821 tgcaccgagc tgtaccagcg caacagtttg cgatgtaact ttgcatcatc aggaatgaac
    47881 cacggacctt ccatcgccaa aaacgcagtg acatagtcag gatggtaacg gcagcaacgt
    47941 tccagcgcga agt
//
LOCUS       DAEVZA010000022        47718 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEVZA010000022 DAEVZA010000000
VERSION     DAEVZA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.022"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..414)
                     /locus_tag="LAF92_001287"
     CDS             complement(<1..414)
                     /locus_tag="LAF92_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI9790324.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVN"
     gene            complement(490..1809)
                     /gene="brnQ"
                     /locus_tag="LAF92_001288"
     CDS             complement(490..1809)
                     /gene="brnQ"
                     /locus_tag="LAF92_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBI9790325.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(2216..3511)
                     /gene="phoR"
                     /locus_tag="LAF92_001289"
     CDS             complement(2216..3511)
                     /gene="phoR"
                     /locus_tag="LAF92_001289"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBI9790326.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(3569..4258)
                     /gene="phoB"
                     /locus_tag="LAF92_001290"
     CDS             complement(3569..4258)
                     /gene="phoB"
                     /locus_tag="LAF92_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBI9790327.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            4448..5650
                     /gene="sbcD"
                     /locus_tag="LAF92_001291"
     CDS             4448..5650
                     /gene="sbcD"
                     /locus_tag="LAF92_001291"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBI9790328.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYT
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            5647..8790
                     /gene="sbcC"
                     /locus_tag="LAF92_001292"
     CDS             5647..8790
                     /gene="sbcC"
                     /locus_tag="LAF92_001292"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBI9790329.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            8916..10100
                     /gene="araJ"
                     /locus_tag="LAF92_001293"
     CDS             8916..10100
                     /gene="araJ"
                     /locus_tag="LAF92_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBI9790330.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(10243..11151)
                     /gene="mak"
                     /locus_tag="LAF92_001294"
     CDS             complement(10243..11151)
                     /gene="mak"
                     /locus_tag="LAF92_001294"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBI9790331.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            11276..12187
                     /gene="rdgC"
                     /locus_tag="LAF92_001295"
     CDS             11276..12187
                     /gene="rdgC"
                     /locus_tag="LAF92_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBI9790332.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(12265..12684)
                     /locus_tag="LAF92_001296"
     CDS             complement(12265..12684)
                     /locus_tag="LAF92_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790333.1"
                     /translation="MYELLTPTINLQIHCTRYPMRSQNGGSTDLPRYWITLDKNVIWD
                     YPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAELLTKQFTSDKWGL
                     VNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(12888..13172)
                     /gene="ppnP"
                     /locus_tag="LAF92_001297"
     CDS             complement(12888..13172)
                     /gene="ppnP"
                     /locus_tag="LAF92_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBI9790334.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(13244..13921)
                     /locus_tag="LAF92_001298"
     CDS             complement(13244..13921)
                     /locus_tag="LAF92_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBI9790335.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(14179..14370)
                     /gene="yaiA"
                     /locus_tag="LAF92_001299"
     CDS             complement(14179..14370)
                     /gene="yaiA"
                     /locus_tag="LAF92_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBI9790336.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(14420..14944)
                     /gene="aroL"
                     /locus_tag="LAF92_001300"
     CDS             complement(14420..14944)
                     /gene="aroL"
                     /locus_tag="LAF92_001300"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBI9790337.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(15127..15585)
                     /locus_tag="LAF92_001301"
     CDS             complement(15127..15585)
                     /locus_tag="LAF92_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBI9790338.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            15705..16514
                     /gene="proC"
                     /locus_tag="LAF92_001302"
     CDS             15705..16514
                     /gene="proC"
                     /locus_tag="LAF92_001302"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBI9790339.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(16531..17646)
                     /gene="adrA"
                     /locus_tag="LAF92_001303"
                     /gene_synonym="dgcC"
     CDS             complement(16531..17646)
                     /gene="adrA"
                     /locus_tag="LAF92_001303"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBI9790340.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(17748..18068)
                     /gene="psiF"
                     /locus_tag="LAF92_001304"
     CDS             complement(17748..18068)
                     /gene="psiF"
                     /locus_tag="LAF92_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBI9790341.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(18187..19602)
                     /gene="phoA"
                     /locus_tag="LAF92_001305"
     CDS             complement(18187..19602)
                     /gene="phoA"
                     /locus_tag="LAF92_001305"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBI9790342.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(19703..19963)
                     /gene="iraP"
                     /locus_tag="LAF92_001306"
     CDS             complement(19703..19963)
                     /gene="iraP"
                     /locus_tag="LAF92_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBI9790343.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(20145..20348)
                     /locus_tag="LAF92_001307"
     CDS             complement(20145..20348)
                     /locus_tag="LAF92_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790344.1"
                     /translation="MLQSSADEACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            20426..21520
                     /gene="ddlA"
                     /locus_tag="LAF92_001308"
     CDS             20426..21520
                     /gene="ddlA"
                     /locus_tag="LAF92_001308"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBI9790345.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGLKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(21544..21756)
                     /locus_tag="LAF92_001309"
     CDS             complement(21544..21756)
                     /locus_tag="LAF92_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBI9790346.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            22016..22324
                     /locus_tag="LAF92_001310"
     CDS             22016..22324
                     /locus_tag="LAF92_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBI9790347.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(22383..23477)
                     /gene="yaiW"
                     /locus_tag="LAF92_001311"
     CDS             complement(22383..23477)
                     /gene="yaiW"
                     /locus_tag="LAF92_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBI9790348.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(23490..24710)
                     /gene="sbmA"
                     /locus_tag="LAF92_001312"
     CDS             complement(23490..24710)
                     /gene="sbmA"
                     /locus_tag="LAF92_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBI9790349.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            25062..26219
                     /gene="ampH"
                     /locus_tag="LAF92_001313"
     CDS             25062..26219
                     /gene="ampH"
                     /locus_tag="LAF92_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBI9790350.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(26220..26843)
                     /gene="iprA"
                     /locus_tag="LAF92_001314"
     CDS             complement(26220..26843)
                     /gene="iprA"
                     /locus_tag="LAF92_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HBI9790351.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(26931..29909)
                     /gene="ehaB"
                     /locus_tag="LAF92_001315"
     CDS             complement(26931..29909)
                     /gene="ehaB"
                     /locus_tag="LAF92_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HBI9790352.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCAERANDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWSDEGTSGWFGNTGNASDYNGGDWNRDDIALAVIAHPAAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHLTVDTNDDGVPDAYGPVYNDNGVVIDTSTGTEATATLIDIAANS
                     LWPSSTVGVENSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINS
                     GSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGD
                     AALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLD
                     ADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVA
                     AATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRG
                     NTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFG
                     GNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDS
                     QTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYD
                     FKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSED
                     QALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANY
                     LGGGDVDQDWSANVGVKYTW"
     gene            30432..31406
                     /gene="hemB"
                     /locus_tag="LAF92_001316"
     CDS             30432..31406
                     /gene="hemB"
                     /locus_tag="LAF92_001316"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBI9790353.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(31512..32363)
                     /gene="tauD"
                     /locus_tag="LAF92_001317"
     CDS             complement(31512..32363)
                     /gene="tauD"
                     /locus_tag="LAF92_001317"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBI9790354.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(32360..33187)
                     /gene="tauC"
                     /locus_tag="LAF92_001318"
     CDS             complement(32360..33187)
                     /gene="tauC"
                     /locus_tag="LAF92_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBI9790355.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(33184..33951)
                     /gene="tauB"
                     /locus_tag="LAF92_001319"
     CDS             complement(33184..33951)
                     /gene="tauB"
                     /locus_tag="LAF92_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBI9790356.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIVHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFISMREYVLSRVFEQREAFS"
     gene            complement(33964..34926)
                     /gene="tauA"
                     /locus_tag="LAF92_001320"
     CDS             complement(33964..34926)
                     /gene="tauA"
                     /locus_tag="LAF92_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI9790357.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTVELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            35542..36213
                     /locus_tag="LAF92_001321"
     CDS             35542..36213
                     /locus_tag="LAF92_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBI9790358.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSELGEGFVI
                     HKMIL"
     gene            36223..37419
                     /locus_tag="LAF92_001322"
     CDS             36223..37419
                     /locus_tag="LAF92_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBI9790359.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            37397..37978
                     /locus_tag="LAF92_001323"
     CDS             37397..37978
                     /locus_tag="LAF92_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBI9790360.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTTISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            37980..38753
                     /locus_tag="LAF92_001324"
     CDS             37980..38753
                     /locus_tag="LAF92_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBI9790361.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            38938..39213
                     /gene="frmR"
                     /locus_tag="LAF92_001325"
     CDS             38938..39213
                     /gene="frmR"
                     /locus_tag="LAF92_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBI9790362.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            39248..40357
                     /gene="frmA"
                     /locus_tag="LAF92_001326"
     CDS             39248..40357
                     /gene="frmA"
                     /locus_tag="LAF92_001326"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBI9790363.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            40450..41283
                     /gene="fghA"
                     /locus_tag="LAF92_001327"
     CDS             40450..41283
                     /gene="fghA"
                     /locus_tag="LAF92_001327"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBI9790364.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(41408..41947)
                     /locus_tag="LAF92_001328"
     CDS             complement(41408..41947)
                     /locus_tag="LAF92_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBI9790365.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(42049..43260)
                     /gene="mhpT"
                     /locus_tag="LAF92_001329"
     CDS             complement(42049..43260)
                     /gene="mhpT"
                     /locus_tag="LAF92_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HBI9790366.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(43436..44449)
                     /gene="dmpG"
                     /locus_tag="LAF92_001330"
     CDS             complement(43436..44449)
                     /gene="dmpG"
                     /locus_tag="LAF92_001330"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HBI9790367.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(44446..45396)
                     /gene="mhpF"
                     /locus_tag="LAF92_001331"
     CDS             complement(44446..45396)
                     /gene="mhpF"
                     /locus_tag="LAF92_001331"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HBI9790368.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(45393..46202)
                     /gene="mhpD"
                     /locus_tag="LAF92_001332"
     CDS             complement(45393..46202)
                     /gene="mhpD"
                     /locus_tag="LAF92_001332"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HBI9790369.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLLGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(46212..47078)
                     /gene="mhpC"
                     /locus_tag="LAF92_001333"
     CDS             complement(46212..47078)
                     /gene="mhpC"
                     /locus_tag="LAF92_001333"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBI9790370.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(47096..>47718)
                     /gene="mhpB"
                     /locus_tag="LAF92_001334"
     CDS             complement(47096..>47718)
                     /gene="mhpB"
                     /locus_tag="LAF92_001334"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="catalyzes the cleavage of 3-(2,3-dihydroxyphenyl)
                     propionate into 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate;
                     part of the 3-phenylpropionic acid degradation pathway;
                     member of the protocatechuate 4,5-dioxygenase family;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBI9790371.1"
                     /translation="YCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTR
                     MLGEAIGRFTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERE
                     LRQQRVISAAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKS
                     THEIKTWVAAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
BASE COUNT        11903 a        12627 c        11733 g        11455 t
ORIGIN      
        1 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
       61 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
      121 ttcaaagcag taggtccatc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
      181 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
      241 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
      301 tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac ccagtgccat
      361 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
      421 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
      481 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
      541 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
      601 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
      661 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
      721 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
      781 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
      841 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
      901 tgcacaagca caggtcaggc caactgccgt taccaggcag gcgatgaaga ttaacgccgc
      961 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
     1021 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
     1081 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
     1141 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
     1201 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
     1261 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccaaacaa tcgcggcaac
     1321 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
     1381 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
     1441 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
     1501 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
     1561 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
     1621 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
     1681 agtccagacg tgttcgcctg cctgcaagcc gaccattgga gggaaaataa tgttacctgc
     1741 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
     1801 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
     1861 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
     1921 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
     1981 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
     2041 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
     2101 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
     2161 aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag gcggattaat
     2221 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
     2281 ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc acgatcgcta
     2341 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
     2401 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
     2461 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
     2521 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
     2581 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
     2641 cctcacgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaaatgcg
     2701 tcggtgcggc ttctattttc gacagcgtca gcaactgctt caccagccct tccatccgct
     2761 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
     2821 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
     2881 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
     2941 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
     3001 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
     3061 ccgggtaacg cagcaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
     3121 gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttcagtg gtcagcacca
     3181 ctgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
     3241 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
     3301 taccacgccc cggtggcggg gtcatactac gatccaccca cagccaccat gaaaggcgca
     3361 ataaattcca gaaatgccag ataagcagtc ccgttaccga tgccagcaaa aaccagggca
     3421 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaagca gcaaagtagc agctccagca
     3481 ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag aaatcataag
     3541 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
     3601 cgcgcacggt ctgcaccatg cggtcatgcc cgcctggctc cagtgcttta cgtaggcgac
     3661 gaatgtggac atcgaccgta cgatcttcca catacacgtt agttccccag acgtggttta
     3721 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
     3781 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
     3841 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
     3901 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
     3961 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
     4021 ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttaatgaac tggataccgg
     4081 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
     4141 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
     4201 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
     4261 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
     4321 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
     4381 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
     4441 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
     4501 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
     4561 tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc cgcccagtta
     4621 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
     4681 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
     4741 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
     4801 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccac gtgacattat
     4861 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
     4921 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
     4981 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
     5041 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacac
     5101 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
     5161 cggctctccc attccactga gttttgatga atgcggaaag agtaaatatg tccatctggt
     5221 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
     5281 catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
     5341 tgtatcacag gagccgcctg tctggctgga tatcgaaatc actactgatg agtatctgca
     5401 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
     5461 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
     5521 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
     5581 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
     5641 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
     5701 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
     5761 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
     5821 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
     5881 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
     5941 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
     6001 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
     6061 ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca ggggcaattt
     6121 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
     6181 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
     6241 gagctggaga agctgcaagc gcaggccagc ggcgtcgcgt tgctcacgcc ggaacaagtg
     6301 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt acttaccgcg
     6361 cagcagcaag aacaacaatc actaaactgg ttaacgcgtc tggacgaatt gcagcaagaa
     6421 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
     6481 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
     6541 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
     6601 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
     6661 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
     6721 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
     6781 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
     6841 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct ggcgcaacat
     6901 gctgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
     6961 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
     7021 cgtaatgccg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
     7081 gatgtgaaaa ccatttgcga gcaggaagcg cgcattaaaa cgctggaagc ccagcgcgcg
     7141 cagttacagg cgggtcagcc ttgcccactt tgtggttcca ccagccaccc ggcggtcgag
     7201 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
     7261 gttaaaaagc tcggtgaaga aggtgcgacg ctacgtgggc aactggacgc cttaacaaag
     7321 cagcttcagc gtgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
     7381 caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca ggacgatatt
     7441 caaccgtggc tagatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
     7501 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
     7561 attgaacaac gccagcagca gctcttaacg gcattggcgg gttatgcact gacattgcca
     7621 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
     7681 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
     7741 gaaacgttgc cgcaaagtga tgagctcccg cacagcgaag aaactgtggc gctggaaaac
     7801 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
     7861 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
     7921 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
     7981 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
     8041 ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
     8101 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
     8161 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
     8221 cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca ggttgaggac
     8281 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
     8341 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
     8401 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
     8461 tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
     8521 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
     8581 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
     8641 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgtagaagcg
     8701 atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct gggctacagc
     8761 aaactggaaa gtacgtttgc agtgaaataa ctattcagca gaataatgaa tacagagggg
     8821 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
     8881 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
     8941 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
     9001 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
     9061 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
     9121 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
     9181 cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat ggcgcatttt
     9241 ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
     9301 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
     9361 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
     9421 cggtgatggc atcggtctat ttttgggtgc cggatattcg cgacgaggcg aaaggaaagc
     9481 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacca
     9541 tgtttggcaa cgcaggtgtg tttgcctggt tcagctacgt aaagccatac atgatgttta
     9601 tttccggttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
     9661 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
     9721 cagtgactga ctttataatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
     9781 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
     9841 cgctacaaat attgttacta caaaacgcca aaggcggaga gttattaggt gccgcaggtg
     9901 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggtggt atgatgctga
     9961 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    10021 tgtcgtcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat actccggtgc
    10081 tggcgaaacc actggggtag gttatagtct cggtgctcat tacttattgc cggatgcggc
    10141 gtgaacgcct tatccgccct acgcggttct ggcacatttt gcaggcctga taagacgcgg
    10201 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    10261 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    10321 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    10381 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    10441 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    10501 gctttcttca accaggctga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    10561 ataatctgtg gcaaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    10621 ataacaaggg acttcctcgc gataacgcag ttcgtcttcg tccatccacg gtagcggatt
    10681 gtgtccccac tcacctgccg tgccattgcc gccgatatgc gcccgcccat tgaatgccac
    10741 gcccgcgccg catcccgtgc cgataatcac ggcaaatact gtctgcgctc ccgctgccgc
    10801 gccatctact gcttctgaaa ccgccagaca gttggcgtca tttgccagcc gcacttcccg
    10861 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    10921 attcttaacc acaccggtgt aaggcgaaat tgagccagga atgcccatac ctaccgttcc
    10981 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    11041 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    11101 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca cggtactctc
    11161 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    11221 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    11281 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    11341 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    11401 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    11461 tcaaattgtt atctgcgcac gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    11521 ggcgctggaa gcgaaaatcg ccaagctgga agcggaacag gcgcgtaagc tgaagaaaac
    11581 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgag ctttcagccg
    11641 ttttagccag acaatgatgt ggatcgacac ggttaatggt ttgattatgg tggactgcgc
    11701 cagcgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    11761 tgttgtacca ttgagcatgg aaaacccgat tgaactgacg ctaaccgaat gggttcgctc
    11821 cggtagcgca gcacagggct tccagttgct tgatgaagcc gagctgaaat cgttgctgga
    11881 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    11941 catcgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    12001 tgtgatgtgc gacgatggtt cgctcaagcg tctgaagttc tgcgacgagc tgcgcgatca
    12061 aaacgaagat atcgaccgtg aagatttcgc ccagcgtttt gatgccgatt tcatcctgat
    12121 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    12181 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    12241 tacgatgcca caccaggcat ataattattc gctacggcgg gcaataattt ttagcgcagc
    12301 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    12361 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacaactc
    12421 cgcttttggg gtgtcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    12481 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccaccgttac ctgcaataaa
    12541 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    12601 ggtgcttcca ccattttggc tgcgcatggg ataacgggta cagtgaattt gcaggttgat
    12661 cgttggcgtc agtaattcat agatctcccg ctgtaacttg ctccaggggg cgttgtgtca
    12721 tcagttccgc aaaaaatacc tggagatctt cactgatatt tatatcaacc ttgatgaatt
    12781 atgaatatac cggaaattga aaaaatcgca gcaaaggaaa tattattatg ggttaaagag
    12841 aggatatttc agaaaaatgc cctcccgttc aaggggaagg cgaggaatta cagatagcgg
    12901 cacagataag aggtgggttc ggcaacttgc agatgaaact cactgtgacc gggaacatta
    12961 aacaccgaac cggcttcata cacctgccag tcggtcgcgt caggcagtaa cacattcagc
    13021 gcgccactga ttaccgtcat ctcttccggc tcagcggtgc tgaaggtgta ttcgccttca
    13081 accataacac ccacgctggc gcgaccagtg ctgctgctgg aaaagccgat tgatttcact
    13141 ttgccggaaa agtactcatt actttgaagc ataaactggc cctgtttggt agttgaacat
    13201 ctacttcact ataggggcca gaggcgctga ctgtcacgca aaattacacc agtaattccg
    13261 cagccagccg tgcaatcaat acgttagaca gcaagacagg aacatcgagc tgtttttgca
    13321 gtaaatcgcg atgacgttgg tgaaatccta aacaatccag catgatgaca tcagcccctt
    13381 ttgccagtaa ttctttcccg gcatcaatga ttttttgttc tgaatcatga atggggttac
    13441 ccaatgaaaa taccggcgat ttctgcaaaa tttgccattt ttgcgcctga acgggcagca
    13501 tctcctcaac cggaacgata acccccacct gatgatcttc aacaatagag gaaaccagtg
    13561 gaggcaatat tcgcgacggc tcaagaaaga tcgtattacg cgcagtcata ctactaatgt
    13621 ttgctgtact cattaataaa atgacgtcat aaccctgatt atcgagcact tcaaccacac
    13681 cttgcaggtc tcgctccact ttacgacgcg aaacatgggc cagctggttg tcatttaata
    13741 atgtgagaat ggtgtcttcg cctgcttctg gcgcgtactc cgccatcact tcttcacgac
    13801 ttaacttccc cagcaggcta tgatgggaaa tattatcttc gtcaatgtat tccgtcagga
    13861 gcggcaaaac ctcctgcata ggtacaatgc cgatggtcag gatcgccaac gacgcactca
    13921 ttgttcatcc accttacttt tctttcgtcg ttaattaccg ggcaagtgtg aagcaccact
    13981 gctgacactt acttgtgcta caaatgacaa aaagcgtagc agcagacgtg cacggcataa
    14041 tgtaaagatt ccaaatgatt ccagtaatgg attgttattg tttaatattc taattattag
    14101 aaaaacatga attatgaaaa atgtgacgca gatcccttgt agtggcactc agaatccctt
    14161 ccgagcagtc tgatgcggtc actctttgtc agggtcctca tagcgttttt tcgcatccat
    14221 cgcttcctga gccgtcggat gttcactgat caacgagtct ggtttaggat gatcggctct
    14281 gagttggtac caggttaccg tctgtcctgg ctttcctttt tcaatcgtca ctatatatgc
    14341 ttcacgagga taaggcggtt tcgttggcat cgttcttcct tatttcacgg gatgaacgtt
    14401 aagtataggc gctctaaaat caacaattga tcgtctgtgc cagggcgcta cgaatttcag
    14461 aaatcacctg gctgggttcg tttgttgcgt cgatgataat atgcgcaact tcgcgatata
    14521 gcgcatcgcg ttcttccagc acttcctgaa cttcttcgct cagcggtttt cccgttaagg
    14581 ttggccgtaa atcttcttcc ggtgcagctt gcagtcggtt aaccaggact gatactggcg
    14641 cacacaaata aaccacgatc ccgttatttt gcataaagtg acgattaaat tccgtcagaa
    14701 taatgccgcc gcctgtagcg ataacggtgg atggcgcagt taccgcttcc agcgccgccg
    14761 tttctctggc gcgaaatccc gcccactctt ccctttcgac gatctccgcg accgtcatat
    14821 tgagctgtga ttgcaaccac tgatcggtat cgacaaaccg acggttaagc gaatcggcaa
    14881 gggccattcc gaccgttgtt ttaccacagc cccgaggccc gatcagaaaa agaggttgtg
    14941 tcatcgtggg ttttccccaa taggtcgcaa tgcggcgaaa gccggtgtca tgagaatagc
    15001 gatcatacca tcaaactagt acaatttcga ttgtaaagaa aaaattccac ttaaagtgaa
    15061 aatctcaata catcccttac tataccaata aatattcaag aatgaagtgt aaataataaa
    15121 ttacatttag ccacgactac gttgcacttc cagccaccac ttctccagct ccgcggcaaa
    15181 ggcctggcgg tcacgttgtg aaaggctatc tggtccgccg gtctggatcc cactggcacg
    15241 taaggtatcc ataaaatcgc gcatcgtcag gcgctcacga atggtcgctg gcgtgtaacg
    15301 ttcgccgcgc ggattaagcg ccgctgcgcc tttctcgatg gcttcagcag ccaaaggtat
    15361 atctgcggtg atcaccaaat cgcccgcttc acactgccgg acaatttcgt tatcggcaac
    15421 gtcgaaacct gccgcgacgc gcagcgtacg aataaatcgc gatggcggca cgcgtaaact
    15481 ctggtttgct accagtacca gcggcatctg catacgttcc gccgcgcgat acaaaatctc
    15541 tttaattaca ttgggacacg cgtcggcatc cacccaaatt gtcataaagt catcctttgt
    15601 agggtaattc tctattgtgt cgcgcttttg ccttccggca tagttctgtt tatgcttctg
    15661 ccagcgatta tcaaaacaat gaatttcacg gcaggagtga ggcaatggaa aagaaaatcg
    15721 gttttattgg ctgcggcaat atgggaaaag ccattctcgg cggtctgatt gccagcggtc
    15781 aggtgcttcc agggcaaatc tgggtataca ccccctcccc ggataaagtc gccgccctgc
    15841 atgaccagtt cggcatcaac gccgcagaat cggcgcaaga agtggcgcaa atcgccgaca
    15901 tcatttttgc tgccgttaaa cctggcatca tgattaaagt gcttagcgaa atcacctcca
    15961 gcctgaataa agactctctg gtcgtttcta ttgctgcagg tgtcacgctc gaccaacttg
    16021 cccgcgcgct gggccatgac cgaaaaatta tccgcgccat gccgaacact cccgcgctgg
    16081 ttaatgccgg gatgacctcc gtaacgccaa acgcgctggt aaccccagaa gataccgctg
    16141 atgtgctgaa tattttccgc tgctttggcg aagcggaagt aattgctgag ccgatgatcc
    16201 acccggtggt cggtgtgagc ggttcttcgc cagcctacgt atttatgttt atcgaagcga
    16261 tggccgacgc cgccgtgctg ggcgggatgc cacgcgccca ggcgtataaa tttgccgctc
    16321 aggcggtaat gggttccgca aaaatggtgc tggaaacggg agaacatccg ggggcactga
    16381 aagatatggt ctgctcaccg ggaggcacca ccattgaagc ggtacgcgta ctggaagaga
    16441 aaggcttccg tgccgcagtg atcgaagcga tgacgaagtg tatggaaaaa tcagaaaaac
    16501 tcagcaaatc ctgatgactt tcgccggacg tcaggccgcc acttcggtgc ggttacgtcc
    16561 ggctttcttt gctttgtaaa gcgccaaatc tgccgatttc aaccactcac gatagtgact
    16621 catttgtggg ttcagcggcg caacccccac actaatccgt aaagttacct gtggcgtatt
    16681 cggcaagcgt aacgtgttta gcccttcacg cacccgtaac atggcggtaa tggcgctctc
    16741 agctggcgta ccggacatga ttactgcaaa ctcatcgccg ccaaaccgac caatcacatc
    16801 gctaccgcgc agggtaattt gtaactgtcg ggtaagcgcc acaatcgctt catcgcccac
    16861 atcatgaccc caggtatcgt tgatgctctt gaaatggtcg atatcgataa tcagtaacgt
    16921 tgcatcgcga ttatgccgcc gacagttatc aaattcattg cgtaacatag tttcccaatg
    16981 acgtcggtta tacacgcccg tcatgccgtc gcgggtactc atgacctgca acctgcgttt
    17041 atgttccgcc agtttggttg ccgtctggta gctgacccag ccaaacagca gaggataaac
    17101 gacaataatg ggaagggaga gccaccattc cagcggcgca ctattgaacg acacggtaat
    17161 gcccgtaagc tcgagggtga caaggcagga aaccaccatc aacaccagac ccgcgacaaa
    17221 cagacggggg ccgcctgccc ccatcaaatt cagacacata atcatcaaca tagcggtgga
    17281 aggcagcacg tttacgccca ttacgcctac ccacattccc gctaatactg catcggtttt
    17341 taagttgtaa atttcccggc taagcggatc gacggccctg ctcgctatct gccaggctaa
    17401 atgcggccag acgaacgccc agccgaccaa caccagccac caccagcccg gcggcgggtg
    17461 cgaaaccagc gttgaagcaa tcggtaagaa catgccagcc aggccaaccg cacggggtag
    17521 tctgacgcga cgggcgaagc gcagcccgga ccgctgatgt tcgttttgag gagttaaagg
    17581 aggttcctcc ccatgcgccg ccgctttttt gaaaaagttt tcatcattca ttatttttgg
    17641 gaacattcag aaacaatttt cccaaattat agagacgggg ctaaaggcag aagtatgaaa
    17701 tttcgggtga gccactttcg tagctcaccc gttagcacta tcaggtatca ggcggctttc
    17761 ttgaggcagg cactcataaa cttattacga tcatcacctt tcagagattg ttgcgttgct
    17821 tgattattac attcgcgcat cttttgctgc tgtggcgtca aacttttttc accaggcgca
    17881 gacttgctgt tcttcaggca atcactcatg taggtcttac gagcatcccc tttcaacgcc
    17941 tgcgccgtcg cctgctgatt acaggatgtc atacgctgtt gttgtggggt taaggttctc
    18001 tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa gcaaggtaac cagtaatgtt
    18061 attttcatag caccatccct cttcatgttt taaccatgag cgtatgcgcc cgtgatctgc
    18121 cattaagtct ggttgctaac agcaaaaaaa ccacccggca gcgaaaattc actgccgggc
    18181 gcggttttat ttcagcccca gggcggcttt catggtgtag aagagatcgg tctggtcggt
    18241 cagtccaaca acattggcgg catgcgggcc atacgccgca atacgcaact gactgccggt
    18301 atgttcttgt gaatcctctt cggagttccc gtagctcatc accatcactg cgccatcttt
    18361 ggtatttagc gcctgggtga ggcccggagc tttggtatcc ggcgcgacaa tctggctggc
    18421 gtgggcgtga tcagcggtga ctatgaccag cgtattgcca tcctttttag cgaattccag
    18481 cgcccgttgt acggcttcat cgagatcgac cgtctcgcca atttgcccac aaggattcgc
    18541 agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc aggaaaaatc ctttctcatt
    18601 tttactcaac aattcaatgg ctttgtcggt catctgcgcc agggttggta cactgtcatt
    18661 acgttgcgga ttaggcgtac aggtgactgc gggcttatcg atattgccgt ggtacgttgc
    18721 tttcggtcct agccagcgca ctggcatatt gccgtcagca aacagtccaa gcagaggttt
    18781 ttgctgattc gcttccgtca ccgaattcag tgaggcagca tcgctgacca actgataacc
    18841 acgcgcctgt gcctgttcac gcagcgtttt tccctgccat tcaccggcgg ttgccgtttc
    18901 agcaaaggtt tttgcgccac cgccaagcgt aacgtcggca cgagcgttaa gcagctgttc
    18961 ggtaatcgat ccttttccgc ctttttccag agcgttaccc ggacattttt cactggtcgc
    19021 gctcggaccg tagcatttgc gcgaagtcac atgtgccacc agcgcagcgg gcgtggcatc
    19081 ctgcaactct gcggtagaaa cgttaccggt cgccagacct gcggcttttg ccatttccag
    19141 aatcgttggg tgatcttttt cgtgaatatc gacgcccagc gcgccgttat aggttttgac
    19201 accggttgac caggcggttg ctgatgcagc cgaatcggtg acgtagtccg gtttgccggt
    19261 ttttttattc agcgcatagt gagtgtattg cccggtaagc ggtaaggcat cgataccttt
    19321 aaaaaagccg cccgcacctt cggcataatt acgtgcggct gtaatttccg aatcccccat
    19381 cccatcgcca atcagcaaaa taatattttt tgcaggttta tcgctaagag aatcacgcag
    19441 agcggcagtc tgatcacccg ttaaacggcg agcaccgccg ggtgcagtaa tatcgccctg
    19501 agcagcccgg ttttccagaa caggcatttc tggtgtccgg gcttttgtca caggggtaaa
    19561 cagtaacgat aagagtgcca gtgcaatagt gctttgtttc actttatttt ctccatgtac
    19621 aaatacatta aaaaataaaa acaaagcgac tataagtctc ggccgtgaca actttatgac
    19681 agctgttgaa aagattaact ttttactgac gaggatgctt caataacttc tttacgtaat
    19741 cgcgcagcag ctccgtatcg tcgtcaggaa tgctggcatc gggctttacc tcgtacagcg
    19801 ccccctctac ctgatcaatc aaccgctgtt ggtcattttg cgccatattg cgaagcattg
    19861 cagtgacgat aatctccaaa gcttctacct gcgcacacag ttctttcgac tcttcttctt
    19921 tttgggcaag cttaaataac aactcagcaa tgagattttt catgtctgta tttccttatc
    19981 caaagtatgg agaagttaac attatgtttc acaacttcat agtgtctgga agtattattt
    20041 ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt gattaccagc gaaacgtttc
    20101 tcttatttaa ataattaccc acacaaaata taggctttta attatcaacc tttattataa
    20161 ataaaagtta tgaaatatat tctcttcgtt aacttataat taagagaaaa aaactcaccg
    20221 catggccatt taagccagga ataaattagc ttcccgttca tcatttttgt gatcttgcgc
    20281 acatctttga gataacgcac tttcttcacc agtacgttgc acgcttcgtc agcagaagat
    20341 tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt cgtggtaatg tatgcacttt
    20401 gtgttagacg ggataggttt ttaagatgga aaaactgcgg gtaggaatcg tttttggtgg
    20461 taaatcagcg gaacatgaag tgtctctgca atcggcaaaa aacattgtcg atgccattga
    20521 taaaagtcgc ttcgacgttg tgctgctggg cattgataaa caagggcaat ggcacgtcag
    20581 cgatgccagc aattatctgc taaatgcaga cgatcctgcc catattgcgt tgcgcccttc
    20641 ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat cagcttatcg acgcgcaaaa
    20701 cggtcagccg ttgccgacgg tggatgtcat tttcccgatt gtccacggta cgctgggcga
    20761 agatggttcc ttgcagggaa tgctgcgggt cgccaattta ccgtttgtag gttctgatgt
    20821 tctggcttca gcagcctgta tggataaaga tgtcaccaaa cgtctgctac gcgatgccgg
    20881 gctgaacatt gcgccattta ttaccctgac gcgcgctaat cgtcacaaca tcagttttgc
    20941 cgaagtggag tctaaactgg ggttaccact gtttgtaaaa ccggctaatc agggctcttc
    21001 tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca attgccgtcg atctggcgtt
    21061 cgagttcgat cataaagtga tcgttgagca agggctcaaa ggtcgtgaga tcgaatgcgc
    21121 agttctgggc aacgacaatc cgcaagccag cacctgtggc gagatcgtac tcaccagcga
    21181 tttctatgcc tacgacacca agtacattga cgaagatggc gcgaaagtgg tagttccggc
    21241 agccattgca ccagagatca acgataagat ccgggcgatt gccgttcagg cttatcagac
    21301 gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta accccagaga acgaagtggt
    21361 gatcaacgag atcaacacac tgcctggctt caccaacatc agtatgtatc cgaagctgtg
    21421 gcaagccagc ggtctgggtt acaccgatct gatcacacgt ttgattgagc tggcgctgga
    21481 gcgtcacgct gcggataacg cattgaaaac cacaatgtga tattttaacg cccgataata
    21541 ttcttattgg gcgttttcag tttcttcatc gcgacgacga ataatcaacc ccgccagcca
    21601 gaaactaatc acccacgtca ccagaccgac ggcataggtc tgccaacctt ttgcttcaaa
    21661 tcccagtaac cccaccacgc cattaagaat gaatataagg ccaatggcga acgcatagta
    21721 gtgccagtca cggcggattt ttacaggcag attcatggca gctccgttaa cgaaaaacaa
    21781 catccttgca tattctgtaa caaccgtctg tggtgtagac aacattttcg taattgttaa
    21841 taaatttaac ttaaggttgc cattttgtga ctcacaacga agattcacgc ttcgggaata
    21901 ttcccaacat caatttacca tgattctgat attgacaatc taaatgacct gtcagacttg
    21961 ccataagccg ctggtatcac accagaagag aagcagatga cttccggagg tttttatggc
    22021 tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat cgcaatgcct cttcaacgcc
    22081 tggcaacatt gcctatgcgt tgtttgtgct gttttgcttt tgggctgggg cgcaattgct
    22141 gaacctgtta gtgcatgcgc ccggcgtcta tgagcgttta atgcaggtcc aggaaacagg
    22201 tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt ttcgggctga tcccgttttt
    22261 agtaggctgc ctcatttttg cagtggtggc gctatggctg cactggcgac atcgccgcca
    22321 gtaagattta cgttgccaga tacggcctaa aacgccttat ctggcatgac attcctgttt
    22381 gatcactgtt tcatacaacg cgcccgccgt tcgtctacgc gcttcgcaaa ccaggccgta
    22441 gtcaggttgc gggtgatttt cgggctttcc agttgaatgc caggcaacat ttcgcggggt
    22501 agtgatttac ccgttttcgc ttcggcaagt tgataaactt tcttgtacag cgccgtctcc
    22561 tcaaaagaga agctgtcacc tttctctaac tgacggcgaa tttcgctgtc gctcatcccc
    22621 agttttcccg ccagtttgcg cgttgccagt tcggttttcc cgggttcttt actgtcgtaa
    22681 cgaattaaat caccgtccag tgccagcttc acgccgctgg ccttactgac tgcgttctga
    22741 aacgcggcat tacggctggc gtaccagcca gcgttaaaat cagcaaagcg gtatatcggt
    22801 gcgctatagc tggcgggata attcagtaaa tggtaagtac caaaccacaa cccgccgcgg
    22861 cgactgaaga cttcctgacg aactgtaccg tccattttcc acggataccc tttggtatgc
    22921 tgttcggcaa aagcaatgct gacctgcatc ggcccaccgg tgcgcacagg attaagcgaa
    22981 ccaaacagcg tctgccccat tggtaccatg ctgatcagat cgtcaaaaat cgcgctcaat
    23041 tgcttttctg tacgcaccga atctaaccgt tcgctataac ttttgccgtt tggcgatttg
    23101 atttttagcg cggtatgaac cagaaaagca ggaatgtgca tccgttcagc acgacggtca
    23161 atctcttgcc aggcgatctt gcttaagccc ggaacggccg gatctgcctg ataatttgac
    23221 tcctgctgcg ccaccgccag caccgagcag acgttttcca gcgtcggtgc cagcccctga
    23281 ctttcaaagg tggtggcgag atctttcgcc caggcatcgc ggtctttcac gctcgcaggc
    23341 atcttctgcc gcacgacgct cgccacatct atcgcttttt cgcccttttt caacggttgc
    23401 ggagcctgac tactacaccc cgctaaaacc agaaccgcca acaatgacag cgaagataga
    23461 ggattgacgc gcgacattac ttctcctttt tagctcaagg tatgggttac ttcctgaatt
    23521 ttgtcaccat ccagttcatg ttcaaagctg cgcagacgtt tgtagataga catcaactca
    23581 accagtgtgg tccatgagtt aatcaggtac tggaaagcac cgcgaacctg accaaaaacg
    23641 ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg caacaattga cggaaacagc
    23701 aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa tgcgggcgat gttgaaatac
    23761 atatagtgaa aatagaggcg aaaatagttt ttccgtacgg cgctaaacag ctcgcgtacc
    23821 gtaggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa ccagctcttt acggtaggcg
    23881 gcctctactc gctggttttt aaactccagc cccggcagtt tgatccctac cactgccagc
    23941 aatccggtcc ccatcagcga ccagacgata gcggcaatca ccagaccata cggaatgtgc
    24001 ccgataatcg gcagctccgg cacatgcgcg gagagcgtta ccagcaccgg cagaaaggcg
    24061 atcaacgtca tgatggcgtt gataaaactg acgcccatat tctccagcgt tgaagcaaaa
    24121 cgcatggtgt cttcctgcac acgctgtgcg gcaccttcga tatgacgcag ttgttgccag
    24181 ttcgccatgt aatattcgtt catcgccgta cgccagcgga acacatagtg actgacaaag
    24241 aagttgttca gtacactgat caccaccgcg atcagcgcaa tccccagaaa gacgcccact
    24301 tcgcggtaaa attgttcgat agtgacttta tgcggcgaac ttagcgcggt ttgaatcaga
    24361 tcatagaacg gtgcatacca ggcgttgacg gcgaccccga cttccaccaa aaaccaggtg
    24421 acgaagatga tcagtgcagt accgagtatt gaccagtatt gccaacggtg cgggctgtag
    24481 ataaaccaga aaaatgcaaa aagtcctacg caaacaatgt agtaagcgta aaaaatcagg
    24541 aaatccaacg accagaaacg cgcggcgcta atcgggatct gcccggaagc gccggtgata
    24601 cgcgccaccc agtcaccccc accggcttgc cagaagataa cggcaatcaa tgcccaaaca
    24661 aaggccgaga gaaaaaacgc tcccggcttt gggaaaaaag acttaaacat atgcactcct
    24721 gctaacttct tatcgtttta acaactgctg tgtattaacc gcatgaccac tacaaagcgt
    24781 cagccaaatg aaaccgccca ttgtgaccca tagcaaggcg cttagttcgc cagcctgacc
    24841 ataagaattg tttcgacgga tttcactaac caaagattgt ttacataagc ggattaacac
    24901 gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa gatcgtgttt acgcgccgtt
    24961 acgtttttaa cactattatt gcactaattc gccctttgca atccatcaat gagtagtata
    25021 aatacgctca gttaccttca ttcaatctat ggacaccacc gttgaaacgt agtctgcttt
    25081 tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca tgcggcgcag ccgatcactg
    25141 aaccggagtt tgcctctgat attgtcgatc gttatgccga tcatattttt tacggcagcg
    25201 gtgccacggg gatggcgctg gttgttatcg acggtaacca gcgcgtcttt cgcagttatg
    25261 gcgaaacgcg acctggtaat aacgttcgcc cgcagctgga ttcggtcgtg cgtattgctt
    25321 ccctcaccaa gctaatgacc agtgaaatgc tggtgaaatt gctcgaccag gggaccgtga
    25381 agttaaacga tccgctaagt aaatatgctc cgccaggcgc acgcgtgcca acctacaatg
    25441 ggacgcccat taccctagtg aatctggcaa cccataccag cgccctgccc cgtgaacagc
    25501 ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac gcgcgagcaa cgctggaaat
    25561 acctttctac ggcgaagctg aaagccgcgc caggcagtca ggcggcgtac tctaaccttg
    25621 cgttcgacct gctggcagat gcactggcga atgcgtccgg caagccttat acccagttgt
    25681 ttgaagagca gattacccgt ccattgggga tgaaagacac cacctacacc ccctcaccgg
    25741 atcagtgccg ccgtttaatg gttgctgagc gcggtgccag tccgtgcaat aacacgctgg
    25801 cggcaattgg cagcggcggc gtctattcca cgcctggcga tatgatgcgc tggatgcagc
    25861 agtatttgtc atctgatttt tatcagcgca gtaaccaggc agatcgcatg cagacgttga
    25921 tttatcagcg cgcgcagttt acgaaagtga ttggcatgga tgtccccggc aaagcggatg
    25981 cgcttggttt aggctgggta tatatggccc cgaaagaggg tcgtccgggg attattcaga
    26041 agacaggcgg tggtggcggt ttcattacct atatggcgat gatcccgcag aagaatattg
    26101 gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt taaaaatatg agcgatggca
    26161 tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt ggttatcccc gcatcctgat
    26221 taatactctg aaggcaaacg gttgatagcg accagtttgc ctttattcat ttcgatatag
    26281 ccgcctttac gcaaagcagc gagaacttct gcgacgacag aacgcgatat gcgtgtacgt
    26341 tgatggatat aattcatcac accaatacgt gatcgcaatt cttcattcca gtcaatcatt
    26401 gataataatg ttgcgcggat ttgttcgtag gaattatgcc caatgagctg cacgtcgcgt
    26461 aattccagaa ttctattttg ccaggctaac cagtaaaaag cgtctcgcca gagttgattt
    26521 tgttcaataa gcgtaatggt ttgtttggct ggtagatgat atcccgtaca atttccttct
    26581 gatattaatt tgtatggtat atcgtttttc attaaaccat cagccagccc cataatataa
    26641 ggagcctggg taataccgat aagtacgttt tcttctctac gcagagagat aacgccctct
    26701 aaaataacga aagtatcttc gctattgaca tcactggaaa atataacttg cttttcatta
    26761 ttaaactcga agcgcgtacc gtatctggac aaacatttat cgagcttacc aaattcctga
    26821 agaggtttaa ctacagataa catttgcgcg tcctttgcag taatgcccgt caaatccttg
    26881 acgggcatta tttagattaa attaccagaa tttcttcgga gtgaagaata ttaccaggta
    26941 tatttaacac ccacgttcgc ggaccagtct tgatctacgt caccaccacc gaggtagtta
    27001 gcatcggtat aggcgctgaa gttcttggtg aagctgaact gagtacccag accaacacgt
    27061 accgcagacc cttcagtacc gttatcgatg gaatcaccgt tcacatcgtt atcgttgtta
    27121 gagtcgtcgt agacgtaagc cagtttgaag tacggagtca gagcctggtc ttcgctgtag
    27181 gtgaaggtat aacctgcatc tacacccagt tcataacgca tgctgtcgta agactgaccg
    27241 tcaactttca tgtcgttgct cagctggtag tcatcaccag actggaacag accagaaatg
    27301 ctgccgtaag gagtcacgta accagcatca cccagtttga agtcgtaacc ggctttcaaa
    27361 ccgaagcccc aagcgtcgga gttggtgcta ccgtcaacgt aagtaccgtt gctcatggtt
    27421 gcagacaggt cgttgttgaa gtgagagtaa ctcaagctac catcaacaaa gacgttgttc
    27481 gcgaagtgag cagaagagta gatgtaggca gtctggctgt cttgatccac ctgaccagaa
    27541 cggtcattca tgtcaccttt agcgaagcct gcagccgcac cgacgatcca cttagcgttg
    27601 ttaccgtcaa ttttggtatc aacaccgacc atgatgccgt taacatcctg atcatagttg
    27661 atggtgccgt tgtcgccgtt gaagttacca ccgaagtagc ttacccatgc gccgccgtta
    27721 tcagccaggc catgacgaga gttggtcaga cgagtaccaa cggtgtcttg ttccaggttc
    27781 cagatattgg tgttcgcaga cgggatgctc agcgccatgt tagcgtagtc ggtcagctcc
    27841 atctgttgca gaacaacggt gttaccgcgc tgttcagcct gataggtgta tgcacccagg
    27901 tcagctttgt tagcagcaga gaaggtcgcg ttgctgtttt tgtcgttgac gtagatcagc
    27961 tcgttgccgt tgtagtcagc gatagaacca gcaccagtag cgttgtcgat acgaactttg
    28021 tagttaccgg ttgcagctgc aacatggtca ccgttgttat caacttcgtt ctgaccagct
    28081 tcgatagaag cagtgttgtc gttatcgccg ttaccgttaa tggtcaggtg accatcagag
    28141 ttcattgcga ttaagccata accgtagttt gacttggtag tatcgttggt acggtcgttg
    28201 gtcaggtctg catcaagtac gaagcggttg ctttcgatgt tgaacacacc agcgtgaatg
    28261 ttaccactgg tatcagtagt gctggtcagg ttcagagtat caacctgcag agcattcgcc
    28321 gcagtgttac cgacatccag aataccgtca ttagtcactt tgatgttgtt ggcatacaga
    28381 gcagcatcac ctacagccca accagcagaa acttcatcag aaatggttac ggtactgttg
    28441 tcgacagtca gtgaatcagt tgcaacatga ccatcttcgt tgatgttcag cgcagaaaga
    28501 cctgtcagag agatagagtc agaaatcagc gttgaatcag caacattaac agttgaacca
    28561 ctgttgatgc tcagagtatc aatcagagaa gttttggtgg tatcccactg tgaaccggta
    28621 ttcagggtca cattgaacag accgctctgg taaacttcgt taccgatgat gtgatcaaat
    28681 tcgctgtatt cagagtcgct gttttctacg ccaacagttg atgaaggcca caggctgtta
    28741 gctgcgatgt cgatcagagt tgcagttgct tcggtgcctg tactggtatc aataacaacc
    28801 ccgttatcat tgtaaacagg accgtaagca tctggcacac catcatcatt ggtatcaaca
    28861 gttaagtgcg cagacattgc cgcaccaacc cacttgctgc cattattcag ggtcagatcc
    28921 aggcgatcag taccatccca accgttagta tctacatcac cgtcagcatc acgatagcta
    28981 tcacgaccat gcgggaagaa gttctcatca aagttacttg agaagaatac atcgcccatc
    29041 agtgtagaat tatcgaatgt tgcagtggtt tgcattgcgt tatcagcagc aggatgtgca
    29101 ataacggcta atgcgatatc atctctattc cagtcaccgc cattgtaatc gctggcattg
    29161 ccggtattac caaaccaacc actagtacct tcatcagacc atgaaccaga ggtcacagta
    29221 gagtctttaa cagtgatggt gttgttaaac acttcgctgc tgttaacacc agtgctaacc
    29281 gtgttgtcgt cgatatcttc ccactcgtaa ccctgagtca gcgtaatacc cgctacgtga
    29341 gagttattag agatggacag atcaacttcc tgatccagag tgatggcagt accgatgtta
    29401 aatacattta caacatgagt gtcagcggca ttattgtagg tgccgttata tgtgtagtgt
    29461 tcgtagttgt cgtcgatcgt tgaattatca acagtcagcg ccagacggtc atggtaataa
    29521 tcattcgcac gttcagcaca atcagttgtc atgcactcgg aataaatcat accgtgaata
    29581 gtgctgtttt tgattgtcag actattggcg ttggtattgg tagaaatacc gtcgtccaga
    29641 tagtaggtag aaatcacgcc gttaacagta gcgttattaa tgaccgggta gatatcgccg
    29701 ttgtaataac tatcagcggc atagttgttc cagcctacat aaccatcata gtaaacgtca
    29761 tcagcatagg tggcgtcgtt gtagtgatgg aaagtattgt aagtttgacc agaaatatcg
    29821 ttggtcgctg cattagcctg agaggtgata gccagagtgc aagccaatgc taattgtgat
    29881 actacaagtt tctttttcca ggagtgcatt tgtcatccct cctcagggac gtaataagtt
    29941 gatccatcaa ttatcaatgc atagaaaaca tattatgttt cgcgctaacg attatgctgc
    30001 gttacagacg aatggttcaa ttattctttc ccctaagtcc gaatgtggac atatataatt
    30061 tatggagaaa aatacacatc aaattgataa aaacaccata tttcaatatg ttgcagataa
    30121 tgttattttt gaaacatacg catcataaat atcacagaca caaaccagac aaatatccta
    30181 tcaatatgca acaaagtttt aattattcaa tttatagcag cacaaccaaa ctccccaaat
    30241 aataaccaag agctattaat aaagattaaa aatattttca gtgaatatga aattattgct
    30301 atttctatta caaagatgtc ctggtctctc tttcattctc ttcttgtcaa catcgcgaca
    30361 actttcgtaa aacactcctg ccccgcttca ggtatactgt gcaccttaat tccacaaaca
    30421 tcaggcagac catgacagac ttaatccaac gccctcgtcg cctgcgcaaa tctcctgcgc
    30481 tgcgcgctat gtttgaagag acaacactta gccttaacga cctggtgttg ccgatctttg
    30541 ttgaagaaga aattgacgac tacaaagccg ttgaagccat gccaggcgtg atgcgcattc
    30601 cagagaaaca tctggcacgc gaaattgaac gcatcgccaa cgccggtatc cgttccgtaa
    30661 tgacttttgg catctctcac cataccgatg aaaccggcag cgacgcctgg cgggaagatg
    30721 gactggtggc gcgtatgtcg cgcatctgca agcagactgt gccagaaatg atcgtcatgt
    30781 cagacacttg tttctgtgaa tacacttctc acggtcactg cggtgtgctg tgcgagcatg
    30841 gcgtcgacaa cgacgcgact ctggaaaatt taggcaagca agccgtggtt gcagctgctg
    30901 caggcgcaga cttcatcgcc ccttccgccg cgatggacgg ccaggtacag gcgattcgtc
    30961 aggcgctgga cgctgcaggc tttaaagata cggcgattat gtcgtattcg accaagttcg
    31021 cctcttcctt ttatggcccg ttccgtgaag ctgccggaag cgcattaaaa ggcgaccgca
    31081 aaagctatca gatgaaccca atgaaccgtc gcgaggcgat tcgtgagtca ctgctggatg
    31141 aagcccaggg cgcagactgc ctgatggtta aacctgctgg agcgtacctc gacatcgtgc
    31201 gtgagctgcg tgaacgtact gaattgccga ttggcgcgta tcaggtgagc ggtgagtacg
    31261 cgatgattaa gttcgccgcg ctggcgggtg ctatagatga agagaaagtc gtgctcgaaa
    31321 gcttaggttc aattaagcgt gcgggtgcgg atctgatttt cagctacttt gcgctggatt
    31381 tggctgagaa gaagattctg cgttaatttt tttcctctca gaaaggggga gttaacctgt
    31441 tgaaagtacg gtgaacgccc cctcacccta accctctccc caagggggcg agggaaccgt
    31501 ccgcactcgt atcaccccgc ccgataaaac ggtttatccc caaggatcgt cgcccgatgc
    31561 ataatccgcc gctgtggcag gtaatcggca ttggcatagt gctgagtcac gcggttatcc
    31621 caaatcgcaa tatcattcgg ttgccagcgc caacgcacct gaaactccgg tttagtgata
    31681 tgggcgaata aaaagcctaa caaggcttcg ctctctttct cgctgacatc gacaattcgc
    31741 gtagtaaagc cttcattcac aaacagcgcc tgtttaccgc tcaccggatg cgttcgcacc
    31801 accggatgta gcaacggagg atttttcgcg accgcctcgc gccagcgttg atgttcctcc
    31861 tcggttttgc ggtatttgta ttccgggaac gatttacgga aatcatgctc cgcacgcagc
    31921 ccgctcagca gctggcggaa gggaacagag agcgcctcat aggccgcaat accgctggtc
    31981 cagagcgtat caccgccggt cgaaggtaac tctttagctg ccagaatcgc ccctgcgggt
    32041 ggcgtttcaa taaatgtcac atcggtatgc cagttgtcgt tatccggcgg attatcgtta
    32101 tgggtatcca gcacgatgat ctcgtcaacc ccttcggcat gagggtaaac agggtgaata
    32161 tgcagttcgc caaaacgctg ggccagcgcg cgttgctgct gcggcgtaat agtttgatcg
    32221 cgcagaaaca ccacctgatg gcgcagcacc gcatggtaaa gctgttcaaa ctgattatcg
    32281 cttaacgggc gcgtcaggtc ggcacccgaa atttgtgcgc cgatatacgg ccccagcggg
    32341 gtaatgctca gacgttcact cattgtactt ctccatgcca gggcgtcaga cggcgctgta
    32401 acgcgcgcaa acctagttct aaaagaaagg cgataatcgc aatcaccgcg attcccgcca
    32461 gcaccacgtc agtcgcgaga aattcacccg ctgactgaac cataaatcct aaaccgcgcg
    32521 tcgcggcaat cagctctgcc gctaccagcg tagaccagcc cacacccaga ccaatgcgta
    32581 atccggtgag gatttccggc agcgcgccgg gcaaaatgac aaaccacagc acctgcgcac
    32641 ggctggcacc cagcgactgg gcggcacgaa tgcgaacctg ctgcgcgctt ttcacccccg
    32701 ccagcgccga catcgccacc ggtgcaaaaa tcgctaaata gatcagtaag atcttcgagg
    32761 tttcaccaat accaaaccag atcaccatca gcggcaaata agccagcggc ggcacgggac
    32821 gataaagttc gattaccgga tccagaatgc cgcgcaccgt cgggcttagc cccatggcga
    32881 tcccgaccgg aataccgatc accaccgccg cgagtagcgc cagcacaatg cgcgtcaggc
    32941 tggctgccag atgctgccac agcgtggcgt ccataaatcc ctgcggtccg gcaatggtga
    33001 gcagttttgc cagtacttgt tgcggcggcg gcaaaaacag cgggctaatc agttgcagcg
    33061 ccgccaccgt ccaccataca gtgagtaaaa ccgctaacgt gccaatgctt aaggtcacct
    33121 gacgcgagag cggccagcgc catttcagcc gccgcgaatg cagtttttca ttaatgagca
    33181 cactcatgag aacgcctccc gttgctcaaa tacgcggctt aaaacatatt cgcgcatgga
    33241 gatgaattgt ggatcggact tgatgctgcg gctcgactct cccgcaacaa agcggcgagc
    33301 aaagttgagc ggcagccgct ccagcacacg gccagggccg gatgaaagca gaaccagttc
    33361 agtcgccata aacaccgctt cttctatatc gtgggtaatc aacagcacct gcttgcccgt
    33421 ctcctgccag agtttcagca gcagagtttg catctggtcg cgggtgaagg cgtccagcgc
    33481 accaaacggt tcgtcgagta ataacagctg gggattcgcc gccagcgcgc gggcaatccc
    33541 cacccgctga cgttgaccac cggaaagctg ccagatatag cgtttttctg cgccttccag
    33601 ccccactttt ttcagcatct ggtgcacgat ttccagtcgc tgcattttct ctatacctgc
    33661 caattgcagg ccgaacgcca cgttgtcctg tacattgcgc cacggtagta gcccttcatt
    33721 ctgaaaaacc accccgcgct ctgctcccgg tccctcaata cgcttacccg ccagttgaat
    33781 gctgccatgc tgataaggca caaaaccggc aatcagattc agcaacgtgg ttttaccgca
    33841 gccggacggc cccagcacca ccagtagctc gccgctttcc agcgtcaggt tgatatcttc
    33901 cagtgccggt ttgccgccat aatcggcgta aagatgagag atttgcagca tctgcgcctc
    33961 cttttattgc acgaagcgcg aggtaacgta ctggctgtaa tcattcgcta cagccgggac
    34021 cttgccctgc tctttcaaaa actgcgcggt gtcgatgatc gctttgttca ccggtccggt
    34081 cagttctacc gtttgttgct gcggcgtcag ataggtattc cccttcacca gccccggaac
    34141 gtcaccttcc ggcacgccgc ttaaacgcgc cagtttgctg atgttttccg gctgtttcag
    34201 ccacgcgtct gggttagcaa tgtacggttg ctgagcatcg atggcgcttt tagcgaacgc
    34261 tttcacgacc tcaggatgtt tctcggcaaa atctttgcgc accacccaga cgtccagcgt
    34321 tggcgcaccc cactgcccga cctgttcaga atcggtcaac accttgccgt ctttttccag
    34381 ggcgttaacc gccggtgccc agacataagc accatcaata tcaccccgct gccatgcggc
    34441 gataatcgcg ggcggctgca ggttcacaat ctccacttgc ccgggtttaa tcccccagtg
    34501 tttcagcgcc gccagcaggc tgtagtgggt ggtggagata aacggtacgg caatgcgttt
    34561 gccaatcaaa tcttcgggtt tgctgatagt tttctttacc accagcgctt cggagttacc
    34621 cagttttgac gccagcaaga agacttcaat cggcacctgt tggctggctg caaccgctaa
    34681 tgggctggaa ccgaggttgc cgatttgcac gtcgccagaa gccagcgccc gcacgatgct
    34741 ggctccgctg tcaaacttac gccagtccac ggttgctccg ctttctttag caaaggtgtt
    34801 gtcggcctga gccactttcg ccggttcggc tgaggtttga tacgccacgg tgacgttcac
    34861 cgcctgtgcc tgaaaagcga tgaatgccag tgcggcaaga agtgtgttac gcgatgaaat
    34921 tgccatgatt gtctgctccc ctgtcttgtt atgtgagcag tattcaggaa taaaaacatt
    34981 cattaaaaga attagtcgtt atcgcacaga tgattttatt cttagcaaaa aaacggcaaa
    35041 ggaaagatta aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt
    35101 ttagtgatgc ataaacaacc acattacgag atttattact tcattatttc tatcaatatc
    35161 aatacatata ataaaactat tatattcgca ttgtatatat cactgtatca ttctcaatca
    35221 ctcgcatatt acattcacat aatgtatata aacaatactt gttcacagaa catatttttt
    35281 aaatgcaatt aattgacaaa ctaaatcaca tctaaataca atgtataaaa caaaataact
    35341 atacatacca aacatacaaa cacttaacat atgaattgca taaaaacatt acccagtcga
    35401 acctccgggt ctgaaagccc ttgcttttca gttattagca acatttaaca tttataaatt
    35461 aacatctggc tgcgccagag attacgtctc tgagtgcatc gttgtgcttg tagtataata
    35521 aataaaatgg agtgtcatta agtggataag gttttagatt cagccctcct ttcttcggca
    35581 aataaaagaa agggtatcct tgctattggc gcacatcctg acgatataga attaggctgt
    35641 ggcgcatcgc ttgctcgtct tgcgcaaaaa ggaatttata tcgcagccgt ggtgatgact
    35701 accggcaact ctggtacaga tggaataatc gatcgccatg aagaatcgcg caacgcccta
    35761 aagatattag ggtgccacca aactattcat cttaattttg ctgacacccg cgctcattta
    35821 cagctcaatg atatgatttc cgctctggaa gacatcatta aaaatcaaat tccttctgat
    35881 gttgaaatca tgcgggtata taccatgcat gatgccgacc gccatcagga tcatctcgct
    35941 gtttatcaag cttcaatggt tgcctgccgc actattccac aaattctcgg ctacgaaacc
    36001 ccaagtacct ggctttcatt tatgcctcag gtttttgaat ccgttaaaga agaatatttc
    36061 acagtcaaac ttgctgcatt aaaaaaacat aaaagccagg aacgacgtga ttatatgcgt
    36121 catgatcgcc tgcgtgcagt tgcacaattt cgcgggcaac aggtcaatag cgaattgggt
    36181 gaaggctttg taattcataa aatgattctt tgagcaaatt gcatgaaaac ctggatattt
    36241 atctgtatgt ccatagcaat gttgctatgg tttttaagta cgctaagacg taaacccagt
    36301 caaaagaaag gctgtattga cgccattata cctgcgtata acgaaggccc gtgtctggcg
    36361 cagtcactgg ataatctact gcgtaaccct tatttttgcc gggtaatttg cgttaacgac
    36421 ggctccacgg acaataccga agcggtcatg gcggaagtca aacgcaaatg gggcgaccgc
    36481 tttgttgccg tcacgcaaaa aaataccggt aaaggtggtg cgctgatgaa tggcctcaat
    36541 tatgccacct gcgaccaggt ttttttaagt gatgccgaca cctatgttcc gcccgatcaa
    36601 gacggaatgg gctatatgct ggcagaaatt gagcgcggtg ctgatgccgt aggcggcatt
    36661 ccctctactg cgttgaaagg cgcggggctg ttaccgcaca tccgcgcgac cgtaaagttg
    36721 ccgatgattg ttatgaagcg cacgctacag cagctcctgg gcggcgcacc gtttattatc
    36781 agcggtgcct gcgggatgtt ccgtactgat gtattgcgta agttcggttt ctcggatcgt
    36841 actaaagtcg aagaccttga tctcacctgg acattagtgg caaacggcta ccgtattcgg
    36901 caggcgaatc gctgcatcgt atacccacag gaatgcaaca gcccgcgtga ggagtggcgt
    36961 cgctggcggc gttggattgt gggatacgcg gtctgtatgc gcctgcataa aagactttta
    37021 tttagccgct tcggtatctt cagtatattt cctatgctgt tggttgtgct ttatggcgtt
    37081 gggatttatc tcactacctg gtttaatgaa ttcatcacca ccgggccgca tggtgtggtg
    37141 ttggcaatgt ttccgcttat ctggatcggc gtagtttgtg ttattggtgc ttttagcgcc
    37201 tggtttcatc gttgctggtt gttggtgcct ttagcgccgc tttccgttgt gtatgtatta
    37261 ttagcttatg ccatctggat tatttatgga cttattgcct tttttactgg acgcgaacct
    37321 cagcgcgaca aacccacccg ctattccgca ctggtggaag cgtcaaccgc ttattcccaa
    37381 ccttctgtca caggaactga aaaactatct gaagcttaat gttaaagaga aaaatattca
    37441 gattgcagac caggtaatta ttgatgaaac tgcaggtgaa gttgttatcg gcgcgaatac
    37501 ccgtatttgt catggtgccg ttattcaggg tccggtagtg attggcgcaa actgcctgat
    37561 aggtaattat gcgtttattc gtcctggcac aacaatcagc aatggtgtaa aaattggttt
    37621 tgccaccgaa attaaaaatg cggttattga agcggaagca acgattggtc cgcaatgttt
    37681 tattgccgac tcggtagttg caaaccaggc atatttgggc gcacaagtac gtaccagtaa
    37741 tcatcgtctg gatgaacagc ctgtgtctgt tcgaactcca gagggaatta tcgctaccgg
    37801 atgcgataaa ttaggttgtt atatcgggca gcgttcacgc cttggcgtac aagttattat
    37861 tttgcctggg cgaattattt ccccgaacac gcaacttggc ccgcgcgtga ttgtagaacg
    37921 taatttacct gccggaactt attcactccg acaagaactt atccgtacag gagattaaaa
    37981 tgataaaacg gacgttatta gcggcggcca tttttagcgc attgcccgct tatgccgggt
    38041 taacttccat taccgcgggc tacgatttta ccgattattc tggcgatcat ggcaaccgta
    38101 atttagcgta tgctgaactg gtggcgaaag ttgaaaacgc aacgctgctt tttaatcttt
    38161 cccaggggcg tcgtgattat gaaactgaac atttcaatgc cactcgcggt cagggtgcgg
    38221 tctggtataa atggaataac tggctgacaa cccgaacggg tattgccttt gcggataata
    38281 cgccggtctt tgcccgccag gattttcgtc aggatattaa cctggccctg ttgccaaaaa
    38341 cgcttttcac gaccggttat cgctacacta aatattacga tgatgtcgaa gtcgatgcct
    38401 ggcaaggcgg cgtatcactc tatactggcc cggtgatcac cagctaccgc tatacccatt
    38461 atgactccag cgatgcaggt ggtagttata gcaatatgat ttccgtgcgt ctgaatgacc
    38521 cgcgcggcac tggttatacg caactatggc taagccgcgg aacaggtgct tacacctatg
    38581 actggacgcc agaaacacgc tacggcagca tgaagagcgt cagtctgcaa cgtattcaac
    38641 cgctgactga gcaacttaat ctcgggctga cggcaggtaa agtgtggtac gacaccccaa
    38701 ccgatgatta taacggtctg caacttgcag cccatctgac ctggaaattc tgattccttc
    38761 tgccgcccgc tatccggggc ggccttccct gccgattagc cccccctttc ctctttgttt
    38821 tccgaccaca ttcaccggat aaattttatt ctccagtgtt atatactata ggggggtatg
    38881 cattgacata tagcataccc ccctatagta tattgcgtgc agatgatgag gtgcgaaatg
    38941 cccagtactc cggaagagaa gaaaaaggtc cttactcgag ttcgtcgtat tcgagggcag
    39001 attgatgctc tggaacggtc gctggagggt gatgccgaat gccgtgccat actccaacag
    39061 atcgctgccg ttcggggcgc ggctaacggg ctgatggcag aagtgcttga aagccatatc
    39121 cgggaaacgt ttgaccgaaa tgactgctac agccgcgaag tcagccaatc cgttgacgac
    39181 actattgaac tggttcgagc ctatcttaaa tagctgaatc tattaccata ttgaggaaga
    39241 gcgagagatg aaatcacgtg ctgccgttgc atttgctccc ggtaaaccgc tggaaatcgt
    39301 tgaaattgac gttgcaccac cgaaaaaagg tgaagtgctg attaaagtca cccataccgg
    39361 cgtttgccat accgacgcat ttaccctctc tggggatgac ccggaaggtg tattcccggt
    39421 ggttctcggt cacgaagggg ccggcgttgt ggttgaagtc ggtgaaggcg taaccagcgt
    39481 caaacctggc gaccatgtga tcccgcttta caccgcggag tgcggcgagt gtgagttctg
    39541 tcgttctggc aaaactaacc tctgtgttgc ggttcgcgaa acccagggta aaggcttgat
    39601 gccagacggc accacccgtt tttcttacaa cgggcagccg ctttatcact acatgggatg
    39661 ctcaacattc agtgaataca ccgtggtcgc ggaagtgtct ctggccaaaa ttaatccaga
    39721 agcaaaccat gaacacgtct gcctgctggg ctgtggcgtg accaccggta ttggcgcggt
    39781 gcacaacaca gctaaagtcc agccaggtga ttctgttgcc gtgtttggtc ttggcgcgat
    39841 tggtctggca gtggttcagg gcgcgcgtca ggcgaaagcg ggacggatta tcgctatcga
    39901 taccaacccg aagaaattcg atctggcgcg tcgcttcggt gctaccgact gcattaaccc
    39961 gaatgactac gacaaaccga ttaaagatgt cctgctggat atcaacaaat ggggtatcga
    40021 ccataccttt gaatgcatcg gtaacgtcaa cgtgatgcgt gcggcgctgg aaagtgcgca
    40081 ccgcggctgg ggtcagtcgg tgatcatcgg ggtggcaggt tctggtcagg aaatttccac
    40141 ccgtccattc cagttggtca ctggtcgcgt atggaaaggt tccgcgtttg gcggcgtgaa
    40201 aggtcgttcc cagttaccgg gtatggttga agatgcgatg aaaggtgata tcgatctgga
    40261 accgtttgtc acgcatacca tgagccttga tgaaattaat gacgccttcg acctgatgca
    40321 tgaaggcaaa tccattcgaa ccgtaattcg ttactgattt cccgcaggta taccccgtcc
    40381 acttcagacg gggttcttaa tactctccct gggcagccgt ccgggggatt aaccgtgaga
    40441 taatgactga tggaactcat tgaaaaacat gccagctttg gcggctggca aaatgtgtat
    40501 cggcattatt cccaatcact gaaatgtgaa atgaatgtcg gcgtctatct cccaccaaaa
    40561 gccgcgaatg aaaaattgcc ggtgctgtac tggctttcgg gcctgacctg caacgagcag
    40621 aatttcatta ctaaatcggg gatgcagcgt tacgcggctg agcacaacat tattgttgtt
    40681 gcgccggaca ccagtccgcg aggcagtcat gtcgcagatg ccgaccgtta cgatctcggg
    40741 caaggtgccg ggttttacct gaacgcaacg caagcgccgt ggaatgaaca ttacaaaatg
    40801 tatgactata tccgcaacga gctgccggat ttagtgatgc aacattttcc ggcaacgacc
    40861 agaaagtcta tctctggtca ttctatgggc gggctgggcg cgctggtgct ggcgttacgt
    40921 aacccagatg aatatgtcag cgtctcggcg ttttcgccca ttgtctcccc atcgcaagta
    40981 ccgtggggac agcaagcctt tgccgcatat cttggtgaaa ataaagatgc ctggttggat
    41041 tacgatccgg tgagtcttat ttcacaaggt caacgcgttg cggaaatcat ggttgatcag
    41101 gggttgagtg atgattttta cgcggaacag ctgcggactc caaatcttga aaagatctgc
    41161 caggagatga atatcaagac gttaatccgt tatcacgagg gttacgacca cagctattat
    41221 tttgtctcca gttttattgg cgagcatatt gcctaccacg ccaataaact gaatatgcgt
    41281 tgataatagt gcacgactgc cggatgcggc gtgaacgcct tatccgccta catttcgccc
    41341 gcaggccgta ggcatgataa gacgcgcata gcgtcgcatc aggcatctgc gcacgactta
    41401 tctatgttta ccacatcaaa tcatcaggca ctttgaagtc ggcatacgga tcgtcttcat
    41461 cctgctcttc ggcactgagc gcgctgtgca acactatact gctggcatca cgctgagcta
    41521 ttttatcggc aacgctggcg ggaataatgg cgtattcacc ttcgctattg ttatcgacca
    41581 acaagcgagc aatcgccaga cgaccattaa tcaactgcgc ctgtgtgagc ttatcgacaa
    41641 aaatcttctt aatcagattg ccgtcagtga agttaaaacc aatatcgcca ttggcaatgg
    41701 tgattcggtt catttcaatg agctgcttaa cctgagcttt atattctttc gccaacgcag
    41761 cttgtttttg ttgctcgcta agctgtttat cacgctcaag ctgtgccttt ttattttctt
    41821 ctaccgccgc ccgagcttca cgcgcctgaa cgcgcgattt tttcgccgtt ctctccacct
    41881 tcgccgcttt tttgctggtg actaatcctg ctttgagcaa ctgctcttgt aaggtaagtt
    41941 ttgccatctt tgtttccaaa cccgctgaat aatttttagc gagtatacct gtaaacgccg
    42001 tgggtgtatt gggggcaagt ccctttcgcc cctgctaaat aaggcgcgtc aggcatcggc
    42061 acacggctgc attcttgatc tccgactcat caaaataaac accgccaacc cagcaacaag
    42121 aatacccggt gcagaagcgg ccattacgcc gaccgtgcca gtgcctaatg ccagcatttt
    42181 cccggccagt aacggaccgc tcatagcccc cagacgccct accgccacgg ctgttcccac
    42241 acctgttgcg cggatctgcg aactgtaaaa caacggtgcc agggcataca aaacgctttg
    42301 cccacctgtc gcaaacaacc ccgcgacaaa tcccgccagc aacataccgt taaatgacga
    42361 caccgttcca agcgccagca gcgaagctaa catgccgcta taaatcagta gcgacatggt
    42421 tactggacgc agcttatcca tcaatgcgcc caacattaac gtcccgcttg ccgcccccat
    42481 ttgcagggca aacatcaccc ctgccgcctg cgatggctgg aatccttgct ccaccaaaag
    42541 tagcggtagc cagttgatca acatataaac caccagcaga gtgaagaaat aacacaacca
    42601 cagcagcagc gtcgcggttg ccgtttctgg cgcaaataag gcacgcagtg gtggcgcagc
    42661 ctgtttttcg ccagcgaaaa ccgctgactc cggcagccag cgcattaata gcggcaccag
    42721 aatcaacggc accacaccac ctacccaaaa caccgtttgc catgctaagt ttgcccccgc
    42781 gaaacccagt gtcgccgcca gcgccgcgcc aatgggaaca ccgcaataca tcaggctcac
    42841 tgccgtccca cgaaaacgtg gacccgcggc ttcagacgtc agggcgataa gattcggcaa
    42901 cgccgccccc agcccgacac cggtcatcag ccgcgcaaag accagtgagg ggaaatccca
    42961 ggcaatcgcc gttgccagtg agaacaaacc aaacagcgca actgagccaa tcaaaatacg
    43021 cttacgacca taacggtctg ccagcatccc gccaaccaac gcgccgggta gcaaaccgag
    43081 tattccggcg ctaaatatcc agcccatttg cattttatcg agtgcgaaag cctgggcgat
    43141 gccacccgcc gcaatgccag ccgcctgaag atccagccct tccatcagag cgaccaaaaa
    43201 acaaagcccg atggtcagca tcaggcggga tgaagatgat gaaggggtac gagtcgacat
    43261 aagatgaacc tcattgcgag tgcaggtgaa tcgcgactga tacattgcca gccgcgtaat
    43321 cagaactatt taatgactgc ctacgggcga aatgtaggcc ggataaggcg ttcacgccgc
    43381 atccggctgt cgtgctggga tgggcataca aaatgcacgt tatcggcagt catcattatt
    43441 tgttgttgcg cagatccagc gccacgtcga cgatcatatc ctcctggccg ccaaccatcc
    43501 ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa gccataacgc gccgccgccg
    43561 tttcacagtg acgcaggaag ctcgagtaaa caccagcgta tcccagcgcc agcgtttcgc
    43621 gatcgactcg taccggtcga tcctgcaacg gacgcaccag gtcgtcggcg gcatccatta
    43681 acgcatagag atcggtccca tgctgccagc ccagtttatc cgcggcggca ataaacactt
    43741 ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag gctggcgtcg attcggtcgc
    43801 agccctcttc caccgccgcg atagagttcg ccacgccaag actcaggtta tggtgagcgt
    43861 gcatgccagt ttgcgtttct ggtttcagct ctgctttcag ggcgcggaaa cggtcacgga
    43921 tatcgctcat gttcatcgca ccgccagaat ccaccacata aatacaggtc gcaccgtagc
    43981 cttccatcag ctttgcctgc ttggcgagat tctccggcgt ggtcatatgg ctcatcatca
    44041 gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg aatatgctgg gcggaaacat
    44101 cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc gccagcctgc caggcatttt
    44161 tcagatcgtg aatagtgccg attcctggca gcaacaacgt cgcgattttg gcgtgcttca
    44221 ccacatccgc cgctgcttca atccattcaa ggtcgctatg tgcgccgaaa ccatagttaa
    44281 agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat cgaatccacg cgggcatcgt
    44341 ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata ctgatgacga atggcgtgca
    44401 taccgtcacg caatgtgacg tccgagatat aaagtttttt accgttcatg ccgcttctcc
    44461 tgccttgcgc gccagtgact gggccatttt ttccgctgtc gccagcgcac tggaagtcat
    44521 aatatcgagg ttgcccgcat aggcaggcag ataatgcgct gcgccttcga cttccagcca
    44581 gaccgctgtt ttcagcccgg agaactgacc cacgcccggc aaattaaccg gtttatcctg
    44641 cgggataact tcaaactgca cgcgctgttt caggcgatat cccggtacgt aagcctgcac
    44701 cgcctcagcc atttcattga ttgaggcttc gatatcatct tgtgaagctt cgtcgctcaa
    44761 tacatacacc gtgtcacgca tcatcaacgg tggctctgct gggttaagca caataatcgc
    44821 cttccctttt gccgcgccgc ccaccacttc aatggctcgg gaagtggttt ccgtaaattc
    44881 atcgatattg gcacgcgtgc caggtccggc agatttactg gcgatagaag cgataatttc
    44941 ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc attggaatgg tggcctggcc
    45001 gccgcaggtg accatgttga cgttcagttg atcgacgttc gcctcgaggt taaccaccgg
    45061 cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt aagcgaatat ccggtttcgc
    45121 ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg ctggtcgcat caaatacaat
    45181 gtcgatatca gcaaattcag gcatgttcat cagcccgatc accccttcat gggtggtggc
    45241 gacgcccata cgtctggcac gcgccagacc gtcggactga ggatcaatgc cgaccatcac
    45301 cgccatctcc agatgctgac cgtgacgcaa aattttaatc atcagatcgg tgccaatgtt
    45361 gccagaaccg ataatggcga ctttacgctt actcatgaca gacttccttt tggggctgcg
    45421 cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat gggcttcaaa acgatcgccc
    45481 gcattcactg ccaccatcgg acctaatgcc ccggtaagaa tgatatctcc ggcgcgcagc
    45541 ggttcaccca ggctggccat tttgcgtgcc agccagacgg cggcattaag cggatgtccc
    45601 aggcattcgc tgccgcgccc gctggaaacc tcttcgttat tacgcgtcat cttcatggcg
    45661 cagtttttca ggtctaaccc cgccggacgt tgcgccggac cgccgatgac atacaccccg
    45721 caggaagcgt tatctgccac ggtatcgaca aactgaatcg accagtcgcg aatgcggctc
    45781 cccaccactt ccagcgccgg aagtacccat tcaatggcgt tatacagttc gtcgaaggtg
    45841 atatcagttg cgggtaaatc gcggttcaac accagtgcga tctccgcttc aatgcggggt
    45901 tgcagaacac gggaaaaagg aatgatttcg ttatcgccat aacacatgtc ggcaaataac
    45961 gtcccaaaat ccggttgatc aacgcccagt tgttgttgca ctttcggatg cgtcaggccc
    46021 actttacgcc ctaccacgcg acgcccctgc acaacgtcat attgcacatt tatgtgctga
    46081 atggcgtagg ccgcttcagc gttatcgata ccaagcagat cgcgcagcgg cgcaatcgct
    46141 tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt gctcaagagt atgcttcgtc
    46201 atgaccattc cttaagggcg tgcgaggaaa ttcagcacca attgattgaa agcgtcggca
    46261 tgttcccact gcgcccagtg accacagtcg cggaagatat gcagttcaga accggcaatg
    46321 ccggacagca gacgcagacc cgcatccatc ggcacaaagc gatcgttgcg cccccagaca
    46381 atcagggttt gcgctttgat ttccgccaga cgtgggccaa aatccgggaa ctgtttcgga
    46441 ttagcttcca ggctcttaac gaagttttcc aggtgatcgc ggcgcgacag catattattc
    46501 aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat cgaaaacgaa gatatccatc
    46561 atcagcttca ggttttcgat agtcggctga cgataaagct gattcagtcg cttaatacct
    46621 tcggttggca tcggcgtaaa caaactcatg ccgcccgtac cgccgcccat cagcaccagt
    46681 ttgccgacgc gctccggcca gttaagggtg aacgccacag aactatggcc gcccatcgag
    46741 ttgcccagca ggtggatttt ggcgatatcc agttgatcca ccacgctttt caggattcgt
    46801 gcattaagat ccgatcgtga accactatta acgatcgaat cgcttttgcc ccaacccgga
    46861 caatccagca ggatcacccg atagcccgcc tctaccagcg gatcgatatt gcggctgaag
    46921 ttcgcccagc cagtagcacc cgggccggaa ccgtgcagca ggacaacggt ttcgtcgcct
    46981 tgtccgcagt cattaaaatg gatgcgcagc gttttacccg cttcttctac attcagaaaa
    47041 cggctggtgg cggcttcggt ttgtggctga taactcatca tcttctcctg catattcagt
    47101 tctctgttct ggcgcttaac gagccaaatc cggcaatcca ctccgggatt gggcggtaat
    47161 aacgcccttc gctacgccag ttgccaaatg tagaaatagc ggcaaaagcg gcgacccagg
    47221 ttttgatttc atgtgtcgac tttccggcaa tggcggaaag ctcttcgtta ctgacggcat
    47281 ccagttcctg tatgcgtccc tgctccagca aagtcatgaa ctggttatcc caaatcgggt
    47341 tgagcggatg cagagttctc tgatcctcaa caaacttctc agcggcgcta atcacccgtt
    47401 gctgacgcaa ttcgcgctca ctggcgggta aatctttccc gctccccaac agacggtcgc
    47461 gcatatgggc atcggctttc gccagttcgg gcaccggcgg ctgatgggaa agcccaccgg
    47521 aacccaggaa cagcacgcgt ttattgagag tgctggtgaa acgtccaatg gcttcaccca
    47581 acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac accgttgatg aacacaggca
    47641 gaactggcac cttatccagc ccgccgagca ggaactccag tggctgggcg aacccgtggt
    47701 ccacctgcat acagtaag
//
LOCUS       DAEVZA010000023        45520 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEVZA010000023 DAEVZA010000000
VERSION     DAEVZA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.023"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..355
                     /locus_tag="LAF92_001335"
     CDS             <1..355
                     /locus_tag="LAF92_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBI9790372.1"
                     /translation="YLENYYRPNLRVKSRDGLTPMLSTIVPKEAIPDPHNLTLRTFVN
                     GELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIATGTPKGLSDVVPGDEVVVEVEG
                     VGRLVNRIVSEETAK"
     gene            352..1818
                     /gene="hpaE"
                     /locus_tag="LAF92_001336"
     CDS             352..1818
                     /gene="hpaE"
                     /locus_tag="LAF92_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBI9790373.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            1820..2671
                     /gene="hpaD"
                     /locus_tag="LAF92_001337"
     CDS             1820..2671
                     /gene="hpaD"
                     /locus_tag="LAF92_001337"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBI9790374.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            2681..3061
                     /locus_tag="LAF92_001338"
     CDS             2681..3061
                     /locus_tag="LAF92_001338"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBI9790375.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            3172..3975
                     /gene="hpaH"
                     /locus_tag="LAF92_001339"
     CDS             3172..3975
                     /gene="hpaH"
                     /locus_tag="LAF92_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBI9790376.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            3986..4774
                     /gene="hpaI"
                     /locus_tag="LAF92_001340"
     CDS             3986..4774
                     /gene="hpaI"
                     /locus_tag="LAF92_001340"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBI9790377.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            4949..6325
                     /gene="hpaX"
                     /locus_tag="LAF92_001341"
     CDS             4949..6325
                     /gene="hpaX"
                     /locus_tag="LAF92_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBI9790378.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            6335..7225
                     /gene="hpaA"
                     /locus_tag="LAF92_001342"
     CDS             6335..7225
                     /gene="hpaA"
                     /locus_tag="LAF92_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBI9790379.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            7476..9038
                     /gene="hpaB"
                     /locus_tag="LAF92_001343"
     CDS             7476..9038
                     /gene="hpaB"
                     /locus_tag="LAF92_001343"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HBI9790380.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            9056..9568
                     /locus_tag="LAF92_001344"
     CDS             9056..9568
                     /locus_tag="LAF92_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBI9790381.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            9961..12111
                     /gene="btsT"
                     /locus_tag="LAF92_001345"
     CDS             9961..12111
                     /gene="btsT"
                     /locus_tag="LAF92_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBI9790382.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            12161..12364
                     /locus_tag="LAF92_001346"
     CDS             12161..12364
                     /locus_tag="LAF92_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBI9790383.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            12375..13331
                     /gene="yjiA"
                     /locus_tag="LAF92_001347"
     CDS             12375..13331
                     /gene="yjiA"
                     /locus_tag="LAF92_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBI9790384.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(13409..15949)
                     /locus_tag="LAF92_001348"
     CDS             complement(13409..15949)
                     /locus_tag="LAF92_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HBI9790385.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(16301..16465)
                     /locus_tag="LAF92_001349"
     CDS             complement(16301..16465)
                     /locus_tag="LAF92_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HBI9790386.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            16597..18009
                     /locus_tag="LAF92_001350"
     CDS             16597..18009
                     /locus_tag="LAF92_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBI9790387.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(18251..19195)
                     /locus_tag="LAF92_001351"
     CDS             complement(18251..19195)
                     /locus_tag="LAF92_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBI9790388.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            19662..20894
                     /gene="mdtM"
                     /locus_tag="LAF92_001352"
     CDS             19662..20894
                     /gene="mdtM"
                     /locus_tag="LAF92_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HBI9790389.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            20935..22215
                     /locus_tag="LAF92_001353"
     CDS             20935..22215
                     /locus_tag="LAF92_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HBI9790390.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRYAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            22331..23482
                     /locus_tag="LAF92_001354"
     CDS             22331..23482
                     /locus_tag="LAF92_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HBI9790391.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAITARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            23492..24259
                     /locus_tag="LAF92_001355"
     CDS             23492..24259
                     /locus_tag="LAF92_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790392.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            24256..24513
                     /locus_tag="LAF92_001356"
     CDS             24256..24513
                     /locus_tag="LAF92_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBI9790393.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            24578..25438
                     /locus_tag="LAF92_001357"
     CDS             24578..25438
                     /locus_tag="LAF92_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HBI9790394.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            25506..26684
                     /locus_tag="LAF92_001358"
     CDS             25506..26684
                     /locus_tag="LAF92_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9790395.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFSWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(26697..27251)
                     /gene="kptA"
                     /locus_tag="LAF92_001359"
     CDS             complement(26697..27251)
                     /gene="kptA"
                     /locus_tag="LAF92_001359"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBI9790396.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            27501..28184
                     /locus_tag="LAF92_001360"
     CDS             27501..28184
                     /locus_tag="LAF92_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790397.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            28181..28642
                     /locus_tag="LAF92_001361"
     CDS             28181..28642
                     /locus_tag="LAF92_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBI9790398.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGTLTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            28655..29827
                     /gene="iadA"
                     /locus_tag="LAF92_001362"
     CDS             28655..29827
                     /gene="iadA"
                     /locus_tag="LAF92_001362"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBI9790399.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            29892..30803
                     /gene="hypT"
                     /locus_tag="LAF92_001363"
                     /gene_synonym="qseD"
     CDS             29892..30803
                     /gene="hypT"
                     /locus_tag="LAF92_001363"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBI9790400.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(30796..31188)
                     /gene="iraD"
                     /locus_tag="LAF92_001364"
     CDS             complement(30796..31188)
                     /gene="iraD"
                     /locus_tag="LAF92_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBI9790401.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPLSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(31185..31268)
                     /gene="idlP"
                     /locus_tag="LAF92_001365"
     CDS             complement(31185..31268)
                     /gene="idlP"
                     /locus_tag="LAF92_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBI9790402.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            31860..32690
                     /locus_tag="LAF92_001366"
     CDS             31860..32690
                     /locus_tag="LAF92_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBI9790403.1"
                     /translation="MSMPLSNAANNQFYINNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLDISKESWPFNYVTETCRESDGITTTGRMLYRNLRI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPSYPVEDYTVHEITLGLGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(32831..33604)
                     /gene="uxuR"
                     /locus_tag="LAF92_001367"
     CDS             complement(32831..33604)
                     /gene="uxuR"
                     /locus_tag="LAF92_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBI9790404.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(33819..35279)
                     /locus_tag="LAF92_001368"
     CDS             complement(33819..35279)
                     /locus_tag="LAF92_001368"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBI9790405.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(35360..36544)
                     /gene="uxuA"
                     /locus_tag="LAF92_001369"
     CDS             complement(35360..36544)
                     /gene="uxuA"
                     /locus_tag="LAF92_001369"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBI9790406.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            36884..38227
                     /gene="gntP"
                     /locus_tag="LAF92_001370"
     CDS             36884..38227
                     /gene="gntP"
                     /locus_tag="LAF92_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBI9790407.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(38470..39372)
                     /gene="fimH"
                     /locus_tag="LAF92_001371"
     CDS             complement(38470..39372)
                     /gene="fimH"
                     /locus_tag="LAF92_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBI9790408.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(39392..39895)
                     /gene="fimG"
                     /locus_tag="LAF92_001372"
     CDS             complement(39392..39895)
                     /gene="fimG"
                     /locus_tag="LAF92_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBI9790409.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NAMVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRATASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRVLTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(39908..40438)
                     /gene="fimF"
                     /locus_tag="LAF92_001373"
     CDS             complement(39908..40438)
                     /gene="fimF"
                     /locus_tag="LAF92_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBI9790410.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(40448..43084)
                     /gene="fimD"
                     /locus_tag="LAF92_001374"
     CDS             complement(40448..43084)
                     /gene="fimD"
                     /locus_tag="LAF92_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBI9790411.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNNGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNIDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(43151..43876)
                     /gene="fimC"
                     /locus_tag="LAF92_001375"
     CDS             complement(43151..43876)
                     /gene="fimC"
                     /locus_tag="LAF92_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBI9790412.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFALKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(43913..44452)
                     /locus_tag="LAF92_001376"
     CDS             complement(43913..44452)
                     /locus_tag="LAF92_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI9790413.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(44517..45065)
                     /gene="fimA"
                     /locus_tag="LAF92_001377"
     CDS             complement(44517..45065)
                     /gene="fimA"
                     /locus_tag="LAF92_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBI9790414.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTAID
                     GTHPNVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT        10651 a        11849 c        11610 g        11410 t
ORIGIN      
        1 ctatctggaa aactactacc gccctaacct gcgggtcaaa agccgcgacg gactgacgcc
       61 gatgctttca accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg
      121 caccttcgtc aacggcgagt tacgccagca aggcaccacc gccgatctga tcttcagcgt
      181 gcccttcctg atcgcctact taagcgaatt tatgaccctg aatccgggcg acatgatcgc
      241 caccggcaca ccaaaaggct tatctgacgt ggtgcctggc gatgaagtag tggtggaagt
      301 agaaggcgtg ggccgcctgg tgaaccgaat tgtgagtgag gaaacagcga aatgaaaaaa
      361 gtaaatcatt ggatcaacgg caaaaatgtt gcaggtaacg actacttcca gaccaccaat
      421 ccggcaacgg gtgaagtgct ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag
      481 gcggtagcgg cagcgaaaga ggcgttcccg aaatgggcca atctgccaat gaaagagcgt
      541 gcgcgcctga tgcgccgtct gggcgatctg atcgaccaga acgtgccaga gatcgccgcg
      601 atggaaaccg cggacacggg cctgccaatc catcagacca aaaatgtgtt gatcccacgc
      661 gcttcccaca actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacctat
      721 ccggttgacg acaagatgct caactacacg ctggtgcagc cggtgggcgt ttgtgcgctg
      781 gtatcgccgt ggaacgtacc gtttatgacc gccacatgga aggtcgcgcc gtgtctggcg
      841 ctgggcaata ccgcggtact gaaaatgtcg gaactctccc cgctgaccgc tgaccgcctg
      901 ggtgagctgg cgctggaagc cggtattccg gcaggcgtgc tgaacgtggt acagggctac
      961 ggcgcaaccg caggggatgc gctggttcgt catcatgacg tacgtgccgt gtcgttcacc
     1021 ggcggtacgg ccaccgggcg caacatcatg aaaaacgccg ggctgaaaaa atactccatg
     1081 gaactgggcg gtaaatcgcc ggtgctgatt tttgaagatg ccgatattga acgcgcgctg
     1141 gacgccgccc tgttcaccat cttctcgatc aacggcgaac gctgcaccgc cggttcgcgc
     1201 atctttattc agcaaagcat ctacccggaa ttcgttaaac gctttgccga acgcgccaac
     1261 cgtctgcgcg tgggcgatcc gaacgatccg aatacccagg ttggcgcgct tatcagccag
     1321 cagcactggg aaaaagtctc cggctatatc cgtctcggca ttgaagaagg cgcaaccctg
     1381 ctggcgggcg gcccggataa accgtccgac ctgcctgcgc acctgaaagg cggcaacttc
     1441 ctgcgcccaa ccgtgctggc agacgttgat aaccgtatgc gcgtcgccca ggaagagatt
     1501 ttcgggccgg tcgcctgcct gctgccgttt aaagacgaag ctgaaggctt acgcctggca
     1561 aacgacgtgg agtacggcct cgcgtcgtac atctggactc aggatgtcag caaagtgctg
     1621 cgtctggcgc gtggcattga agctggcatg gtgttcgtca acacccagaa cgtgcgtgat
     1681 ttgcgccagc cgtttggcgg cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac
     1741 agcttcgaag tgttcgcgga aatgaagaac gtctgcattt ccatgggcga ccatccaatt
     1801 ccgaaatggg gagtctgata tgggtaagtt agcgttagcc gccaagatca cgcacgtacc
     1861 gtcgatgtat ctctctgaac tgccagggaa aaaccacggt tgccgccagg gcgcgatcga
     1921 cgggcataaa gagatcagca agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt
     1981 cgatacccac tggctggtca acagtgctta tcacatcaac tgtgcagacc attttgaagg
     2041 cgtctacacc agcaacgagc taccgcattt tatccgcgat atgacctaca actacgaggg
     2101 caacccggag ttggggcagc ttattgccga tgaagcctta aagctcggcg tgcgggcaaa
     2161 agcgcacaac attcccagcc tgaaactgga atacggcacg ctggtgccaa tgcgctacat
     2221 gaatgaagac aagcacttca aagtggtttc catttcagct ttctgcactg ttcacgattt
     2281 tgccgacagc cgcaagctgg gcgaggcgat tctgaaagcg atcgaacagt acgacggcac
     2341 cgtggcggtc cttgccagcg gttcgttatc gcaccgcttt attgacgatc agcgagcgga
     2401 agaagggatg aacagctaca cccgcgagtt cgaccgccag atggacgagc gtgtggtgaa
     2461 gctgtggcgc gaaggccagt tcaaagagtt ctgcaatatg ctgccggagt acgccgacta
     2521 ctgctacggc gaaggcaata tgcacgacac ggtgatgctg ctggggatgc tcggctggga
     2581 taaatacgac ggcaaggtgg agtttatcac cgagctattc ccaagctctg gcaccggtca
     2641 ggttaacgct gttttcccgc ttcccgcgta aggacgtttt atgccgcact ttatcgttga
     2701 atgcagtgac aacatccgcg aagaagccga cctgccgggg ttgttcgcca aagtgaatcc
     2761 gacgctggca gccacgggta tttttccgct ggcgggtatt cgcagccgcg tgcattgggt
     2821 cgatacctgg cagatggccg acgggcagcg tgattatgcc ttcgtgcata tgacgttgaa
     2881 aatcggcgca ggtcgcagcc tggaaagccg tcagcaggcg ggtgaaatgc tgtttgaact
     2941 gattaaaacg cacttcgccg ccctgatgga gagccgcctg ctggcgttgt cgtttgagat
     3001 tgaagagctg catccgacgc tgaattttaa acaaaacaac gtgcacgcat tgtttaagta
     3061 gcgcgcagat tgcccggtgg cgctgcgctt accgggccta caaaacccca aaccgtacac
     3121 cgtaggccgg ataaggcgca gccgcatccg gcaatgccac aggatatcgc tatgttcgat
     3181 aaacataccc acaccctgat cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag
     3241 atccgcgcga tctcactgga ttacccggag atcaccatcg aagacgctta cgcggtgcag
     3301 cgcgaatggg ttcgcctgaa aatcgccgaa ggtcgcacgc tgaaaggcca caaaatcggc
     3361 ctgacctcga aagcgatgca ggcaagctcg cagatcagcg aaccggatta cggcgcgctg
     3421 ctggatgaca tgttcttcca cgatggcagc gatatcccga ccgatcgctt tatcgtgccg
     3481 cgcattgaag tggagctggc tttcgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg
     3541 ctgttcgacg tctacaacgc cacggactac gtgatcccgg cgctggagct gatcgacgct
     3601 cgctgccaca acatcgatcc ggaaacccag cgcccgcgca aagtgttcga caccatttct
     3661 gataacgccg ccaacgccgg ggtgatcctc ggtggtcgtc ccattaagcc cgatgagttg
     3721 gatctgcgtt ggatctccgc cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc
     3781 gccgctggtg tgctgaatca tccggcaaac ggcgtggcct ggctggcgaa caaactcgca
     3841 ccgtatgacg tacaactgga agccgggcaa atcattctcg gcggttcgtt cacccgcccg
     3901 gttccggcgc gtaagggcga caccttccac gtcgattacg gcaacatggg ctccattagc
     3961 tgccgctttg tttaaggaga gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg
     4021 ccgcccgcag atcggattat ggctggggct gagtagcagc tacagcgcag agttactggc
     4081 cggagcagga ttcgactggt tattgatcga cggtgagcac gcgccgaata acgtgcaaac
     4141 cgtgctcacc cagctacagg cgattgcgcc ctaccccagc cagccggtgg tacgtccgtc
     4201 gtggaacgat ccggtgcaaa tcaaacaact gctggacgtc ggcacacaaa ccttgctggt
     4261 gccgatggta caaaacgctg acgaagcccg tgaagcggta cgcgccaccc gttatccccc
     4321 cgccggtatt cgcggtgtgg gcagtgcgct ggcccgagcc tcgcgctgga atcgtattcc
     4381 tgattacctg caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga
     4441 ggcaatgaag aacttaccgc agattctgga cgtggaaggc gtcgacggcg tgtttatcgg
     4501 cccggcggat ctgagcgccg atatgggtta tgccggtaat ccgcagcacc cggaagtaca
     4561 ggccgccatt gagcaggcga tcgtgcagat ccgtgaatcg ggcaaagcgc cggggatcct
     4621 gatcgccaat gagcaactgg caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt
     4681 cggcgttgac accaccctgc tcgcccgcgc cgctgaagcg ctggcagcac ggtttggtgc
     4741 gcaggccacc gccgtgaagc ccggcgtgta ttaatgccgg atgcgctgcg cttatccggc
     4801 ctacactcgc accaaaccgt aggccggata aggcgtttac gccgcttccg gcaaaaagct
     4861 gtaccaaatc gcggaccgga taaggcgtta cgccgcatcc gccataaacc ttgtcgtacc
     4921 ctacaaaaat cccattagag gaagaaaaat gagcgacacc tcacctgcca taccggagag
     4981 tatcgatccg gcgaatcagc ataaagcgct gactgccgga caacaggcgg ttattaagaa
     5041 gctatttcgc cgcctgatcg tctttctgtt cgtgctgttt atcttctcgt tccttgatcg
     5101 catcaacatc ggctttgccg gactcacgat gggacgcgac ctcggtctga gcgccaccat
     5161 gtttggcctc gctaccaccc tgttctatgc cgcttatgtc atcttcggca ttcccagcaa
     5221 cattatgctg agtattgtcg gtgcgcggcg ctggatcgcc accatcatgg tgctctgggg
     5281 catcgcctct actgccacca tgtttgccac tggccccacc agcttgtacg tactgcgtat
     5341 actggttggc attaccgaag ccggctttct gcctggtatt ctgctgtatt taaccttctg
     5401 gtttccggcc tatttccgcg cccgtgccaa cgccttgttt atggtagcaa tgccggtaac
     5461 gacagcgttg ggctcgatcg tttccggcta cattttgtcg ctggatggcg tcatggcatt
     5521 aaaaggctgg cagtggctgt ttttgctgga aggcttcccg tcggtattac tcggcgtcat
     5581 ggtgtggttc tggcttgatg actcaccgga caaagctaag tggctgacga aagaagacaa
     5641 aaaatgcctg caagagatga tggataacga tcgtctgacg ctggttcagc cagagggagc
     5701 catcagccac cacgccatgc aacaacgcag tatgtggcgg gagatcttca ctccggtggt
     5761 gatgatgtat accctggcgt atttctgcct gaccaacaca cttagtgcga tcagcatctg
     5821 gacaccgcag atcctgcaaa gttttaatca gggcagcagt aatatcacca tcggcctgct
     5881 ggccgccgta ccgcagattt gtaccattct cgggatggtc tactggagcc gtcactcaga
     5941 tcgccgccag gaacgaaggc atcacaccgc ccttccttat ttgttcgctg ccgcaggttg
     6001 gttactggct tcggcaactg atcacaacat gatccagatg ctggggatca ttatggcttc
     6061 gaccggatca ttcagcgcaa tggcgatttt ctggacaaca ccggatcagt ccatcagcct
     6121 gcgggcacga gcgatcggta ttgcggtgat caacgccact ggcaacattg gttcagcgtt
     6181 aagtccgttt atgatcggct ggttgaaaga tttgaccggc agctttaaca gtggattgtg
     6241 gtttgttgcc gcgctgctgg tgattggtgc ggggattatc tgggcgattc caatgcagtc
     6301 ctcccgtccg cgagcgaccc cgtaaggaac gacgatgtgt gaccgtcaga ttgccaatat
     6361 tgatatcagc aaagagtacg atgaaagcct gggcacggac gatgtgcatt atcagtcgtt
     6421 cgcccgcatg gcggcctttt ttggccgcca tatgctgcca catcgccacg aacagtactt
     6481 tcagatgcat ttcctcaata gcggacagat tgagctacaa cttgacgatc atcgctactc
     6541 ggtggaagcg cccctgtttg tcctgacgcc gccgtcagta cctcatgcgt ttattacgga
     6601 gtctgacgcc gacggtcatg tgttgacggt acgggaagat ctgatctggc ccctgctgga
     6661 agttctttat ccgggcactc gggaaacctt cggcctgccg gggatttgcc tgtcactggc
     6721 agataaaccc gacgaactgg cggcgctgga acactattgg caactgatag agcgggaatc
     6781 ggtagaacaa ctgcctggac gggaacacac cctgacgtta ctggcacagg cagtattcac
     6841 cctactgctg cgtaatgcaa aactcgacga ccatgccgcc agcggaatgc gcggagaatt
     6901 aaaactgttc cagcgtttta atatgctgat tgaaagccat tttcatcagc actggacagt
     6961 accggattac gctaacgaac tgcatatcac cgaatcacgc ctcacggaca tctgccgccg
     7021 ctttgccaac cgtccaccaa aacggttgat tttcgacagg cagctgcgag aagccaaacg
     7081 gctgctgctg ttttctgata acgcagtgaa caatattgcc tggcaactcg gttttaaaga
     7141 tccggcttat tttgcccgct tttttaatcg cttagtcggt tgctcgccca gtgcttatcg
     7201 cgccaaaaaa gtacctgtga cgtgaaaatc ccttagcctt aacgggaaac caggcaccac
     7261 ctgctattcc cctttctccg cctggagcaa ggggatttag taacgtcaaa aatcgctaaa
     7321 agcgacctcg atcacaaaac cgtacccgac ctgaaaagta ccagccattg tcccaaaagt
     7381 ctcttcccgt aatcgcccct gcaagcttca attgaataac aaaaacaaca cacaaaatta
     7441 acaatcgtat tccgattaat actgtagagg tcgacatgaa accagaagat ttccgcgcca
     7501 gtacccaacg tccgttcacc ggggaagagt atctgaaaag cctgcaggat ggtcgcgaga
     7561 tctatatcta tggcgagcga gtgaaagacg tcactactca tccggcattt cgtaatgcgg
     7621 cagcgtctgt tgcccaactg tacgacgcgc tacacaaacc ggagatgcag gactctctgt
     7681 gctggaacac cgacaccggc agcggcggct atacccataa attcttccgc gtggcgaaaa
     7741 gtgccgacga cctgcgccag caacgcgatg ccatcgctga gtggtcacgc ctgagctatg
     7801 gctggatggg ccgtacccca gactacaaag ctgctttcgg ttgcgcactg ggcgcgaatc
     7861 cgggctttta cggtcagttc gagcagaacg cccgtaactg gtacacccgt attcaggaaa
     7921 ctggcctcta ctttaaccac gcgattgtta acccaccgat cgatcgtcat ttgccgaccg
     7981 ataaagtaaa agacgtttac atcaagctgg aaaaagagac tgacgccggg attatcgtca
     8041 gcggtgcgaa agtggttgcc accaactcgg cgctgactca ctacaacatg attggcttcg
     8101 gctcggcaca agtgatgggc gaaaacccgg acttcgcact gatgttcgtt gcgccaatgg
     8161 atgccgatgg cgtgaaatta atctcccgcg cctcttatga gatggtcgcg ggtgctaccg
     8221 gctcgccgta cgactacccg ctctccagcc gctttgatga gaatgatgcg attctggtga
     8281 tggataacgt gctgatccca tgggaaaacg tactgatcta ccgcgatttt gatcgctgcc
     8341 gtcgctggac gatggaaggc ggtttcgccc gtatgtatcc tctgcaagcc tgtgtgcgcc
     8401 tggcagtgaa actcgacttc attacggcac tgctgaaaaa atcactcgaa tgtaccggca
     8461 ccctggagtt ccgtggtgtg caggccgatc tcggtgaagt ggtggcgtgg cgcaacacct
     8521 tctgggcatt gagtgactcg atgtgttctg aagcgacgcc gtgggtcaac ggggcttatt
     8581 taccggatca tgccgcacta caaacctatc gcgtactggc accaatggcc tacgcgaaga
     8641 tcaaaaacat tatcgaacgc aacgttacca gtggcctgat ctatctccct tccagtgccc
     8701 gtgacctgaa caatccgcag atcgaccagt atctggcgaa gtatgtgcgc ggttcgaacg
     8761 ggatggatca cgtccagcgc atcaagatcc tcaaactgat gtgggatgcc attggcagcg
     8821 agtttggtgg tcgtcacgaa ctgtatgaaa tcaactactc cggtagccag gatgagattc
     8881 gcctgcagtg tctgcgccag gcacaaagct ccggcaatat ggacaagatg atggcgatgg
     8941 ttgatcgctg cctgtcggaa tacgaccaga acggctggac tgtgcctcac ctgcacaaca
     9001 acgacgatat caacatgctg gataagctgc tgaaataacg cagcaggagg ttaagatgca
     9061 attagatgaa caacgcctgc gctttcgtga cgcgatggcc agcctgtcgg cagcggtaaa
     9121 tattatcacc accgagggcg acgccggaca atgcgggatt acggcaacgg ctgtctgctc
     9181 ggtcacggat acaccaccgt cgctgatggt gtgcattaac gccaacagtg cgatgaaccc
     9241 ggtttttcag ggcaacggca agttgtgcgt caacgtcctc aaccatgagc aggaactgat
     9301 ggcacgccac ttcgcgggca tgacaggcat ggcgatggaa gagcgtttta gcctctcatg
     9361 ctggcaaaaa ggtccgctgg cgcagccggt gctgaaaggt tcgctggcca gtcttgaagg
     9421 tgagatccgc gatgtgcagg caattggcac acatctggtg tatctggtgg agattaaaaa
     9481 catcatcctc agtgcagaag gtcacggact tatctacttt aaacgccgtt tccatccggt
     9541 gatgctggaa atggaagctg cgatttaagc tccagcccct tcacgcattc gtcgaagggg
     9601 catttttatt gtgatctcaa tcacatatcc acccgatgaa taattcttgc ccaaccgctc
     9661 accgccattc ctgaccgctt acgtccctaa aataaccact cagttattta ccttacttta
     9721 cgcgcgcgta actctggcaa catcactgca ggatagcggt caatttacct cctcaaacgc
     9781 aacgcaaacc tagaacggct tcggcctatg attaacaaat tactctatga attttggttt
     9841 gcaggaaggc agcaagtgag tgaatccccg ggagcttaca acagtaagtg acaggggtga
     9901 acgaacgcaa ctgccgcacc tgtaagctaa aagacgacga gtaaaatgcc aggttttact
     9961 atggatacga aaaaactatt caagcacata ccctgggtga ttctcggaat catcggtgca
    10021 ttctgcctcg cggtagttgc attacgtcgg ggggagcacg tcagcgccct gtggatcgtg
    10081 gtcgcctctg tgtcggtgta tctggtggcg tatcgctact acagtctgta catcgcccag
    10141 aaggtgatga aactcgaccc cacgcgcgcg acgcctgcgg ttattaacaa cgacggtctg
    10201 aactacgttc cgaccaaccg ttacgtgttg tttggtcacc acttcgccgc tatcgccggt
    10261 gctggtccgc tggtcggtcc ggttctcgcc gcacagatgg gctacctgcc tggcacgctg
    10321 tggctgctgg cgggggtagt actggccggt gcggttcagg actttatggt gctgtttatc
    10381 tcctctcgcc gtaacggcgc atctctcggt gagatgataa aagaagagat ggggccagtt
    10441 ccggggacta tcgcgctgtt tggctgtttc ttaatcatga tcatcatcct cgccgttctg
    10501 gcgctgattg tggttaaagc cctggccgaa agtccgtggg gtgtcttcac cgtttgctca
    10561 accgtaccga ttgcgctgtt tatgggtatc tacatgcgct ttatccgtcc ggggcgtgtg
    10621 ggtgaagtct ctgtcattgg tatcgtgctg ctggttgcct ctatctactt cggtggcgtg
    10681 attgctcacg atccgtactg gggtccggca ctgaccttta aagacaccac cattaccttc
    10741 gcgctgattg gctatgcgtt tgtttccgca ctgctgccgg tgtggctgat cctcgcaccg
    10801 cgcgactatc tggcaacctt cctgaaaatc ggcgttatcg tcggcctggc gttgggtatc
    10861 gtggtgctga acccggaact gaaaatgcct gcgatgaccc agtacattga cggtactggc
    10921 ccgctgtgga aaggcgctct gttcccgttc ctgttcatca ccatcgcctg tggtgcggta
    10981 tctggcttcc acgcgctgat ctcttccggt acgacgccga aactgctggc taacgaaacc
    11041 gacgcgcgtt tcattggcta cggcgcaatg ctgatggagt ccttcgtggc gattatggcg
    11101 ctggttgcag cgtccatcat cgaaccgggt ctttacttcg cgatgaatac cccgcctgct
    11161 ggccttggca tcaccatgcc taacctgcat gaaatgggtg gcgagaacgc gccgatcatc
    11221 atggcgcagc tgaaagacgt taccgcacac gcggcagcga ccgtcagctc ctggggcttc
    11281 gtgatttcgc cagagcagat cctgcaaacc gcgaaagaca ttggtgagcc ttctgtcctg
    11341 aaccgtgcag gtggtgcgcc aacgctggcg gtaggtatcg ctcacgtgtt ccacaaagtg
    11401 ctgccgatgg ctgacatggg cttctggtat cacttcggta ttctgttcga agccctgttc
    11461 atcctgaccg cgctggatgc gggtactcgt tctggccgct ttatgctgca agacctgctg
    11521 ggtaacttca tcccgttcct gaaaaaaacc gactctctgg ttgccggtat catcggtact
    11581 gcgggctgtg tgggtctgtg gggctacctg ctgtatcagg gcgtggtcga tccgctgggc
    11641 ggcgttaaga gcctgtggcc gctgttcggt atttccaacc agatgctggc agccgtagcg
    11701 ctggtactgg gcaccgttgt gctgattaag atgaagcgca cccaatacat ctgggtaact
    11761 gttgttccgg ctgtatggct gcttatctgc accacctggg cgctgggtct gaaactgttc
    11821 agcaccaacc cgcagatgga aggcttcttc tacatggcaa gccagtacaa agagaagatt
    11881 gctaacggta ctgacctgac ggcgcagcag attgccaaca tgaaccacat cgttgtgaac
    11941 aactacacca acgcaggcct gagtattctg ttcctgattg tggtgtacag catcatcttc
    12001 tacggtttca aaacctggct tgcggtgcgt aacagcgaca aacgtactga caaagaaact
    12061 ccgtacgttc caatcccgga aggcggcgtg aagatctctt cgcaccacta accgtgttta
    12121 gccccgcttc ggcggggctt tgttcttatc agagtgaact atgtttggta acttaggaca
    12181 ggcaaaaaaa tatctcggcc aggcggcgaa gatgttgatt ggcattccag actatgacaa
    12241 ctacgtcgag catatgaaga ccaaccatcc cgacaagccg tacatgagct atgaagaatt
    12301 cttccgcgaa cgccagaacg cacgctacgg cggcgatggt aaaggcggta tgcgctgttg
    12361 ttaatggaga gagcatgaac ccgattgcag ttaccctact caccggtttt ttaggcgcgg
    12421 gcaaaaccac cctgctgcgc catattctta atgaacaaca cggctacaag attgccgtga
    12481 ttgaaaacga attcggcgaa gtctccgttg atgatcaatt gattggtgat cgcgcaacgc
    12541 agatcaaaac gctgaccaac ggctgcatct gctgttcgcg ctccaacgag ctggaggacg
    12601 cgctactcga cctgctggat aatctcgaca agggcaatat tcagttcgat cgtctggtca
    12661 ttgaatgcac cggcatggcc gatcctggcc cgattattca aacctttttc tcccatgaga
    12721 ttttatgcca gcgctacctg ctggacggcg tgattgcgct ggtggatgcg gtacatgccg
    12781 atgagcagat gaaccagttc accatcgccc agtcccaggt gggctacgcc gaccgtattc
    12841 tgctgactaa aaccgacgtc gcaggcgaag cagaaaaact gcgtgaacgc ctggcgcgca
    12901 tcaacgcccg tgcaccggtc tacaccgtca cccacggcga catcgacctg ggcctgctgt
    12961 tcaacaccaa cggttttatg ctggaagaaa acgtcgtcag caccaaaccg cgtttccact
    13021 ttatcgcgga taaacaaaac gatatttcgt cgattgtggt ggaactggat tacccggtag
    13081 atatcagcga agtttcccgg gtgatggaaa acctgctgct ggaatcggcg gataaactgc
    13141 tgcgttacaa agggatgctg tggattgacg gcgaacctaa ccgcctgctg ttccagggcg
    13201 tccagcgcct ctacagcgcc gactgggacc gcccgtgggg cgatgaaaca ccgcatagca
    13261 cgatggtgtt tatcgggatt cagttgccgg aggatgagat tcgggcggcg tttgcgggac
    13321 tcaaaaagta accagccctc caacatcaca atacctgaga ctttatcacc ctcatattcc
    13381 tccagaatat gagggctttc gggcttaatt atctctgaac gtgcaacact tcctgcgact
    13441 ccttatgcgc ctccacatcc tgcttcgtta accagagcga cttacggcca aaattttcgt
    13501 acattttcag ctgatcttca tagtgcttat caactgttcc atcgggagca ataaacccac
    13561 tctgaccggg tgcgaccaca tcccaggcaa gcacaggacg atcgcttgtc gttggtgaga
    13621 aaacaatcat atcgttttct gttccacggt tttgatactc cgcctgatga cgcgtttctt
    13681 ccgctgcggc ctgcggtaca ccaaagaaat tatttgcccg gaacgttaag gccattgcag
    13741 gtgttttcca gttactcaca ttattgccat agcgtttgga aagagtctcc caggtatctt
    13801 ccagcgcagc caacacaacc tcctgctgtg gtttcccagc aaacagatca accgcctgtg
    13861 ggattggtga tttgtctccc tgcaccgcct catacaaaat ttttgctcca acacttatat
    13921 tcagcgaacc agttgggccg tcctgggttg tttcgtagcc actggcgctg taccacttat
    13981 caaatggcat aggtacggca gccactacgg tacgcttcaa catactggtc agccaaacgt
    14041 tcaggatggc agagcctggc tgctgccagg ttttaccatc atcattaagc aaattgatgc
    14101 catcccaacg tgttaatgtt tctaccaact gacgacgcgg atcgctctgt gtcaaaccag
    14161 atgtcgctgc ttgcagagta ggtaaaaaaa gcctcaggtt aagatcctga cgactggttt
    14221 ggcgaataac atcccatgcc tgatcagcag ttaagcgtgg cttttgctca agcagtcggt
    14281 cgatctccgt aacgcgatct gcaccacccc acaaaaaggc aaacagatct gaagcgggat
    14341 aatctttttg gggagaattg ttccagttag caatatatcc cgactggggg ttatacacct
    14401 tagggttcat ttcaaaaggc aatagccctt tccagtccca ttttcccgta ccaggaacgg
    14461 gtaatcgcgg atcatggcct gattgacgat ctggataagc accagtatga acataaccaa
    14521 tattgccgtt tacatcagca tagtaccagt tgatggtcag tgcttgtttc gctgcctgct
    14581 gtgtccactc ctgccaattt ttggccttca tctgatgagt ccaggccagc aaagacgcca
    14641 cctctttacc atcccatgcg cgggatttag cgtaagccgt ttgtgtcgtc tggtcagttt
    14701 ggagaatgtt gccatgcacc gtacgccaga cagtaaaggt ctctgcctga ccatttttca
    14761 ccgtaatggt ttcctcacgg cttaacattt tcacccactt accattatgc aagtagtagc
    14821 ctggtttctc tgccgacagc cgttcagcaa aaatatcgac atcatcgccg aaacctgccg
    14881 ttgatcccca ggaaatcaca ccattatgac caaaaaccag cccaggatag gcaaatggtg
    14941 tattgccagt gacatcataa ccagcaccgt gcagaccaat accataagta tacgcaggcg
    15001 cataccagcc aaactgcgga ccatttacca tgattgcttt cgcatcctgg gctttgcttt
    15061 tgccgatcac ccacatattg ctggtcgttg gataccccgc cagaccattg gcaccaccct
    15121 gtgcaaattg tgcagcaata gtttcccggt tcttccctgc tgttaacgcc agcagtgcgc
    15181 catccgcccc ttttgctggt cggtcaagca ttggtgcagg taaatcgtag cgtggcaaca
    15241 gagctgctgt ttgcgagttt tgctgattaa atttaagtgg gtagttactc tcttgtacgg
    15301 caatagtggt tggcgctgat gggtttacca gccatttcaa ctgattaaat accgccatgc
    15361 cttgtgatac accatattta tcttttaaag ccgttagcag tgccagatta tcaatttcgc
    15421 tagtgctatc agagaagcgg tttgccatgg tgcccacaaa tatcatcgcg acatcaaacg
    15481 gttcccagcg cttaggagta aagccaaatg tattaaactg ttttggtaag agcgtctctg
    15541 gattggtatt taccttatca atccaggcat tcattccatc agcgtagcct tgcagaatgg
    15601 acatatcctc tggggaaagg gcagcaattt gcgcccggat agcatccggc cagtagttac
    15661 gacggatatc tttatcaaat ttcacaaaat ctttgccaag cacttccgcg acagtccctt
    15721 gagtactgcg acgtgccatt tccatctgaa aaaggcgatc ttgtgctact acatagccat
    15781 agccataaaa taggtgccat gtatcattgg cataaatatg cggcatgccg tattcatcgc
    15841 gaacaatctt tatctcactt gacgactgct cagccagtgc aggtaagctc caataataca
    15901 tcagggaagc agtaacacag ttcacgatca tacgatttct atttttcatt gtatcctctg
    15961 gcaggtgtat aattagcgaa ttgatactag caacgaagct tcggttatct atttgcagag
    16021 aattgtttca ttatgaactg tgagcgcgat aactaatttt tgatacaaaa atttcattaa
    16081 tgtgatctaa aacgaatcgc tttattcacc gctgatgatt ttgttctatt cctttttgtg
    16141 acagatcccc cggacgcata gatgatgacc cccagagaga cagctcaggt cattatctgt
    16201 atgtaaatca gaaaaatatg ttgtaaacga cttttatggt tggattttgt agcaattttg
    16261 aatgcagaca ggcagcattt gccaacacgc cgcccatagt tcactccaca tcctccctgc
    16321 gtaacccaat atccctcaac cgttcatcac tcatctgctg taacactcgt cgggtctgca
    16381 tttgccgccg ccaacgcctc accgcatgcc agagttgcac aagtctgata aacggcgttt
    16441 tggctttgtt ttcgtgaaat tccatctgct gcctcctcat ctttcagagg cttcatcatc
    16501 cgccttctgg cagttcacaa tacagatgca aatattagtt ttatttaaca tacagacagg
    16561 tttttctttc tgtatgggcc aaataaggaa tacagcatga cgcgttatca acatctggcg
    16621 actctacttg ccgagcggat tgagcaaggg ctgtatcgtc acggggagaa attgccgtcg
    16681 gtgcgcagct taagtcagga gcacggcgtc agcatcagca ccgtgcaaca ggcgtatcag
    16741 acgctggaaa cgatgaggct catcactccg cagccgcgtt cgggttattt tgtcgcacaa
    16801 cgtaaagccc agccgcctgt accgccgatg acgcgtccgg tgcagcgccc ggtggaaatt
    16861 acccagtggg atcaggtgct ggatatgctg gaggcgcata gcgacaattc cattgttccg
    16921 ttaagcaaaa gcacgccgga tgtcgaaacg cccagcctga aaccgctgtg gcgtgagcta
    16981 agccgggtgg tgcagcataa tctgcaaacc gttctcggtt atgacttgtt agccggtcag
    17041 cgagtgttgc gggagcagat tgcccgcctg atgctcgaca gcggctcggt ggtcaccgcc
    17101 gatgacatca tcatcaccag cggctgccat aactcgatgt cgctggcgtt aatggcggtg
    17161 tgtaaaccgg gcgatattgt cgcggtcgaa tctccctgtt attacggttc gatgcagatg
    17221 ctgcgaggca tgggcgtgaa agtgattgaa atcccaaccg atccagaaac tggcatcagc
    17281 gttgaagcac tggaactggc gctggaacag tggccgatta aaggcatcat tctggtgcca
    17341 aactgtaata atccgctggg atttattatg ccggacgcac gcaaacgggc cgttctctct
    17401 ctcgctcagc gtcatgatat tgtgattttt gaagatgatg tctacggcga actggcaacg
    17461 gagtatccgc gcccgcggac catccattca tgggatatcg acgggcgagt gctgttgtgc
    17521 agctcgttca gtaaaagtat tgctccaggc ctgcgcgtgg gttgggtcgc accggggcgt
    17581 tatcacgata aactgatgca tatgaaatac gccatcagca gctttaatgt gccgtccacg
    17641 caaatggcgg cggcaacgtt tgtgttggaa ggccactatc atcgccatat ccggcggatg
    17701 cggcagactt atcagcgcaa tctggcgctt tatacctgct ggatacggga atattttccc
    17761 tgcgaaatct gtattacgcg cccgaaaggc ggatttttac tgtggataga attgcctgaa
    17821 caggtcgata tggtctgcgt tgcgcggcag ctgtgccgca tgaatatcca ggtggcagca
    17881 ggctcgattt tctcggcttc cggcaaatac cgtaattgtc tacgcatcaa ctgcgctttg
    17941 ccgctcagcg aaacctatcg cgaagcacta aagcaaattg gcgaggccgt gtatcgggca
    18001 atggaataaa ttcgaattag tggcgaagca cctgatttcc tctcacgaat cgcctgtaac
    18061 tcgttgtatc tttgcagggt catcgccacg ttccagcatg gcttaggcaa agtgtgcttc
    18121 gttcataccg gatgcggcgt gaacgcctta tccggcctac aaaccgcgca aattcaatag
    18181 attgcagagg aaatgtaggt ctgataagcg tagcgcatca ggcagctttg cgtttgtcat
    18241 ccgtctctga ttagtattcg atccatggat cgcgcttgat ttcaggcaag ggctgctcta
    18301 atgcctgcat ttgctctgtc gaaagtccgg taatcttttg tatccattca ggatctgccc
    18361 cattctgcaa caacaatcta aggaggcgtt gttccccttc ttctttcccc ttttccatcc
    18421 ccaacagcca tccatcatta taaattcgct ctgcaatcgt cattattcgc tccctgtgtt
    18481 gtggcattcg acgggtaagt tcgctgataa acttcttaaa acgcgcttca tcgccagtca
    18541 gtaaaatgta atttaacagc gcagttatct ggctgtcatt agcacactct gtaaccagta
    18601 atattaccag ttgatcgata agccccatca gatcgcgctg gcgaatatgc ttttggatca
    18661 actccaacag ggcgactctg cggtgctgca caatctcgtc gtctggcacg acagtgacat
    18721 ccaccagcgg gaacgctgcg gtataaagct tccgtgcggt ggtcgggtca gcaaattcgt
    18781 ccagccagca cagggaccag ggataaggac tacggctacc gtgataaaac agcatcggga
    18841 tgaccagcgg tagcggccgg cgtttatcat gctcgatatg gcgctgcatc accgccatgg
    18901 aatatcgcat caggcgaaag gccatatgga tatcctcgcg gctctgatgt tcaatcacta
    18961 cgtaaatata accatcacct tcacgggtct ttaccgacca cagaatatcg gagtgtagcg
    19021 cccgcaattt ttcatcgacg aagctggcgg attccagttt taagctgtcg agatcgcaca
    19081 gttcacgtaa atctttgggt aagtggatct ccataaaatc ccgcgcggtg tcagggtgcg
    19141 tgagaaagga tttaaataat gcgtcatgcg gcgtgctggt tgtgaagttt gtcatggcga
    19201 tccgtcacct gtagataaga tggcccgact gtagcgacta acaaaaccat tctcacccgg
    19261 caatttctcg ttccccgagg cgctttccag gctatttgtg ttcctctgca aacgatacat
    19321 ttttccggaa acaggctcgc gttttacatt tcctgtaccc ggcagatatt cccctttcca
    19381 ccgcgctctt ttgattaacc gcaaatttta attacactta aggtgtatat tttctatgca
    19441 acccatcaat taaagaggtg taatgtgctg atgactattt gtaatcgtta tacattctga
    19501 cctggagtca gaaggtattt ctctgtctgt gtgttcacag gcagtgtggt tgattacatg
    19561 aaattcagta catttgtcct tctcgcctgc ctttcgtcat taccgacggt attgaatttc
    19621 gttttccccg ttggggttct ccggacaagg agttgtttgt tatgccacgt ttttttgccc
    19681 gccatgccgc tacgctgttt ttcccgatgg cgttgatttt gtatgacttt gccgcctatc
    19741 tttcgacgga tctgatccag ccggggatca ttaatgtggt ccgtgatttt aatgccgatg
    19801 tcagtctggc tccggctgcc gtcagtctct atctcgcggg gggtatggcg ttacagtggc
    19861 tgctggggcc gctttccgac aggattggcc gcaggccggt gctgattacc ggggcgctaa
    19921 tatttaccct tgcctgcgct gcgacaatgt tcacaacgtc tatgacacag tttcttatcg
    19981 cgcgtgcaat tcagggcacc agtatctgtt ttattgctac cgttggttat gtcacggtgc
    20041 aggaggcgtt cggacagaca aaagggatta agttgatggc gattatcacc tccatcgtac
    20101 tgattgcgcc gattattggc ccgctttccg gcgcagctct gatgcacttt gtgcactgga
    20161 aagtcctttt tgccatcatt gcggttatgg ggtttatctc atttgttggc ttactgctgg
    20221 cgatgccaga gacggtgaag cgcggcgcgg ttccatttag cgccaaaagc gtcttgcgcg
    20281 attttcgtaa tgtcttttgc aaccggctgt tcctctttgg cgcagcaacc atctctttaa
    20341 gctatatccc gatgatgagt tgggtggctg tctcgccggt gatcctgatt gacgcaggcg
    20401 gcttaacaac ttcgcagttc gcctggacac aggttccggt gttcggcgcg gtgattgtcg
    20461 cgaatgccat cgtggcgcgt ttcgttaaag atccgaccga accgcgcttt atctggcgtg
    20521 ctgtacccat tcaactggtc ggcctcgcgc tgttgattat cggcaatctg ctgtcaccgc
    20581 acgtctggct gtggtcggtg ctgggcacca gtttgtatgc tttcgggatt ggtttgatct
    20641 tcccgacctt attccgcttt acgctttttt ccaataactt accgaaagga accgtctccg
    20701 catcgctgaa tatggtgatc ctgatggtga tgtctgtctc ggtcgaaatc ggccgctggc
    20761 tgtggtttaa cggcggtcgc ttgccgtttc atctgttagc cgtcgtggcg ggcgttatcg
    20821 tcgttttcac cctggcagga ttgctcaatc gcgtgcgcca acatcaggct gccgagctgg
    20881 cggaggagcg gtgatttttg cgcgatcctg ccgtcaggct ctattcttaa cgttatgaat
    20941 aaactcattg aactcagacg cgccaaaatg ttggcgctct ctttactgct tatcgccgct
    21001 gctacctttg tcgttacgct gtttttgccg cccaattttt gggtgagcgg cgtgaaggcg
    21061 attgctgaag cggcgatggt cggcgcgctg gcggactggt ttgcggtggt ggcgctgttt
    21121 cgccgcgtgc cgattccgat catttctcgt catacggcga ttatcccgcg taataaagac
    21181 cggattggcg aaaatctcgg ccagttcgtg caggaaaaat ttctcgatac ccagtcgctg
    21241 gtggcattga ttcgacgtca cgaaccggcg ttgttgattg gcaactggtt tagtcagccg
    21301 gagaacgccc gccgcgttgg tcagcatctg ttgcagatca tgagcggttt tcttgaactg
    21361 accgatgatg cgcgtattca gcgcctgctt aagcgcgcgg tccatcgggc gattgataag
    21421 gtcgatcttt ccggcaccag tgcgttgatg ctggagagta tgaccaaaaa cgatcgtcat
    21481 caggtgctgc tggatacgct gatcgcgcag ttgattgctc ttctccagcg cgataaatcg
    21541 cgcaagttta ttgcccagca aattgttcgc tggctggaga gcgagcatcc gctgaaagcc
    21601 aaaattttgc ctactgaatg gctgggcgaa catagcgcgg agttggtttc tgacgcggtg
    21661 aattctttgc ttgatgatat cagccgtgat cgtgcgcatc agatccgtta tgcgtttgat
    21721 cgcgccactt ttgccctgat cgacaagttg aaaaacgatc cggaaatggc agcgcgagcc
    21781 gatgccgtaa aaagttatct gaaagaagat gaagctttta accgctatct cagtgaattg
    21841 tggggggatt tacgggagtg gctgaaagcg gatatcaaca gtgaagattc tcgtgtgaaa
    21901 gaacgtatcg cgcgggcggg tcaatggttt ggcgaaacgt taattgccga tgatgccttg
    21961 cgggcgtcgt taaatggtca cctggaacaa gccgcgcacc gcgtcgcgcc tgagttttcc
    22021 gcattcctga cgcgccacat cagcgataca gtaaaaagct gggatgcgcg ggatatgtca
    22081 cggcaaatcg agttaaatat cggcaaagat ctgcagttta tccgtgtcaa cggtacgctg
    22141 gttggcggtt gtattgggct aattttatat ttgttgtcgc agctcccggc cttgttcccc
    22201 ctcggcaatt tttagaaatc catgaaaaac taagtggcta accgcgagag agatcaaata
    22261 aaccacacta aaagaatggt aataatgtgg cctcttttca ttatctcccg tggtacgggg
    22321 aaggaaaatc atgtcactta tcaccgatct acccgccatt ttcgatcagt tctctgaagc
    22381 tcgccagaaa ggctttctca ccgtcatgga tctcaaggag cgcggcattc cgctggttgg
    22441 cacttactgc acctttatgc cgcaagagat cccgatggca gccggtgcgg ttgtggtttc
    22501 gctctgttcc acctctgatg aaaccattga agaagcggag aaagatctgc cgcgcaacct
    22561 ctgcccgctg attaaaagta gctacggctt cggcaaaacc gataaatgcc cctacttcta
    22621 cttttcggat ctggtggtcg gtgaaaccac ctgcgacggc aaaaagaaaa tgtatgaata
    22681 catggcggag tttaagcctg ttcatgtgat gcagctgccg aacagcgtta aggacgatgc
    22741 ctcgcgtgca ttatggaaag ccgagatgct gcgcttgcaa aaaacggtag aagaacgttt
    22801 tgggcacgag attagcgaag atgctctgcg cgatgccatt gcgctgaaaa accgcgaacg
    22861 tcgcgcactg gctaattttt atcatcttgg gcagttaaat cctccggcgc ttagcggcag
    22921 cgacattctg aaagtggttt acggcgcaac cttccggttc gataaagagg cgttgatcaa
    22981 tgaactggac gcgataaccg cccgcgttcg tcagcagtgg gaagaaggcc agcgacttga
    23041 cccgcgtcca cgcattttaa tcaccggctg cccgattggc ggcgcagcag aaaaagtggt
    23101 gcgggcgatt gaagagaatg gcggctgggt tgtcggttat gaaaactgca ccggggcgaa
    23161 agcgaccgag caatgcgtgg cagaaacggg cgatgtctac gacgcgctgg cggataaata
    23221 tctggcgatt ggctgctcct gtgtttcgcc gaacgatcag cgcctgcaaa tgctcagcca
    23281 gatggtggaa gaatatcagg tcgatggcgt agttgatgtg attttgcagg cgtgccatac
    23341 ctacgcggtg gaatcgctgg cgattaaacg tcatgtgcgt cagcagcaca acattcctta
    23401 tatcgctatt gaaacagact actccacctc agatgtcggg cagctcagta cccgtgtcgc
    23461 ggcctttatt gagatgctgt aaggagtggc agtgacatat tcgattggca ttgattctgg
    23521 ttctaccgcc accaaaggga tcttactggc agacggcgtg attacgcgcc gtttcctcgt
    23581 tccaacaccc tttcgtccgg caacagcaat tactgaagcc tgggaaactc tgcgcgaagg
    23641 gttagagaca acgccgtttc tgacgctcac cggctatgga cggcaactgg tggattttgc
    23701 cgataaacaa gtgacggaaa tctcctgtca cgggctgggc gcacggtttc ttgcgccagc
    23761 aacgcgcgcg gtaatcgaca tcggtggtca ggacagcaaa gtgattcagc ttgatgatga
    23821 cggtaacctg tgcgatttcc tgatgaatga caaatgcgcg gcgggcaccg ggcgtttcct
    23881 ggaggtgatc tcgcgcacgc ttggcaccag cgtcgagcaa ctcgacagca ttaccgaaaa
    23941 tgtcacgccg catgccatca cgagtatgtg cacagtgttt gccgaatcag aagtgatcag
    24001 cctgcgctca gcgggcgtcg cgccagaagc gattctcgcc ggagtgatta acgcgatggc
    24061 gcggaggagt gccaatttca ttgctcgtct ctcctgtgaa gcgccgattc tgtttactgg
    24121 tggcgttagt cattgccaga cgtttgcccg gatgctggaa tctcacctgg gaatgccggt
    24181 aaatacccac cctgatgcgc aatttgctgg cgcaattggc gcggcggtaa ttggtcaacg
    24241 agtgaggaca cgccgatgaa agagttttta tttttatttc actccacagt cggcgtcata
    24301 caaacccgca aagcgttgca ggcagcgggc atgacttttc gcgtcagcga tattccccgt
    24361 gatttacgcg gcggctgcgg gttatgcatt tggctgacct gtccccccgg cgaggaaata
    24421 caatgggtga tccctgggct gactgagtca atttattgtc agcaggatgg tgtctggcgc
    24481 tgcattgcac attaccgggt ttctccgcgt taaggattca ttaaggtcgc tacgtatact
    24541 tctcgatatc ttattgattt gccttaaagg gattaccgtg acaaaaagta tttcgttaag
    24601 taaacgcatc tccgttattg tgattctttt tgccattgtg gcagtgtgta ctttcttcgt
    24661 gcaatcttgc gcccgtaaaa gcaatcatgc agcatcattt cagaattacc acgccaccat
    24721 tgacggcaaa gaaattgcag gcataacgaa taatatctct tcgctgacct ggtcggcaca
    24781 aagcaatact ctttttagca ctatcaacaa acctgccgcc attgttgaaa tgaccaccaa
    24841 tggtgatttt atccgcacaa ttcctctgga ttttgttaag gatctcgaga cgatcgaata
    24901 cattggcgat aatcagtttg tcattagcga tgagcgcgat tacgccattt atgtgatttc
    24961 actgacccca aactcggaag taaaaatcct caaaaaaata aaaatcccgc tgcaagactc
    25021 cccaaccaat tgcggttttg aagggttggc ttattctcgc caggatcaca ctttctggtt
    25081 ttttaaagag aaaaatccga ttgaggtcta caaagttaac gggctgttaa gtagcaatga
    25141 attacacatc agcaaagacg aggcactaca acggcaattt actttagatg atgtgtccgg
    25201 cgcagaattt aaccagcaaa aaaatacgct tctggtgctg tctcatgaat ctcgcgcact
    25261 ccaggaagta accctggttg gcgaggttat cggtgaaata tcactaacca aaggtagtcg
    25321 aggtttgtcg cataacatca aacaggccga agggattgcg atggatgcct ctggtaatat
    25381 ttatatcgtt agcgaaccaa atcgttttta tcgctttacc cctaaatcat cgcattaaca
    25441 ggctggcata tttcaccttt ttgttattaa cttgttaaca acaaaaatga catacaaggt
    25501 taaacatgcc ttcgtcctcg catcccgtag aacgcttttc tttcagcacc gcgcttttcg
    25561 ggatgctggt tctgacccta ggtatgggat taggtcgctt cctttatacg cctatgttgc
    25621 ccgtcatgat ggcggaaggg gcgttttcat ttagccagct ctcgtggatt gccagcggca
    25681 actatgcggg gtatctggct ggcagtctgc tattttcgtt tggcgcattt caccagccgt
    25741 cgcgcctacg cccatttctg ttggtttctg ccctggcgag cgggttgttg atcctcgcaa
    25801 tggcatggtt gccgccattt attctggtgt tattgattcg cttcctggcg ggtgtcgcca
    25861 gcgccggtat gctgattttt ggttcgacgc tgattatgca gcacacgcgc catccttttg
    25921 tgctggcagc tttgttttct ggcgttggca ttggcattgc actgggcaac gaatatgttc
    25981 tggcaggcct gcattttgcc ttatcctcgc aaacgttatg gcaaggcgcc ggggcgcttt
    26041 ctggcatgat gctgattgca cttacgcttt taatgccctc gaaaaaacac gccatcgcac
    26101 caatgccatt ggcaaaaacg gagcaacaga taatgagctg gtggttactg gctattctgt
    26161 acggcctggc gggatttggt tatatcatcg tcgcgactta cctgccgctc atggcgaaag
    26221 atgcaggctc accattgtta accgcccatc tctggacgtt agtcggctta tcaattgtgc
    26281 caggctgctt tagctggcta tgggcagcaa aacgttgggg ggctttgcct tgcctgacgg
    26341 cgaatttgct ggtgcaggct atcagtgtgc tgcttactct cgccagcgac tctcctctct
    26401 tgcttatcat tagtagtatc ggttttggcg gtacctttat gggcacgaca tctctggtga
    26461 tgactatcgc ccgccaactg agcgttccgg gaaatctcaa tcttttaggc tttgtgacgc
    26521 tcatttacgg cattggacaa atcctcggcc cggcgctgac cagtatgctc ggcaatggaa
    26581 catcggcgct tgccggcgcc actctctgcg gcgcggcggc gttgtttatt gctgcgctta
    26641 tcagcacagt acaattattt aagctgcaag tggtcacttc atgaggagtg tttcccttac
    26701 cactcaagaa attcgaccgc aaccgttgat gtcagccaga ctccgttttc cgcttgccag
    26761 aaagggatgc ctcgtttcgc catttcctgt gctttgacgg ttaagataac cggcgatccg
    26821 tgtcttgctc ccactttgcg tgccgttgct tcatcagcag agagatgcac gtaatgacgc
    26881 tcccctgcaa tcagcccctg tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg
    26941 taaagaaact gtggcggtgt tttttcggca aaggaaatag cgacctgcga agtcgaatgc
    27001 ccctgaaccg cgcggatgca cctaccatca ctggaataac taaaacgctt tttatcactg
    27061 gtggcgacca cggtatccag caatgtacgg gttaatcgct tgcccgcttt ttgggcacaa
    27121 agtatgagct tatcgatatc cgcccatcct tcacggtcaa gtacgatacc aatcgcctcc
    27181 ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat ccgccagctc tttttcgttg
    27241 tactttgcca tctcgttttc gtccctggat tttctgtttt tatttactga tgtcattgtc
    27301 actgggttta tgaattttct gcaactcaat cctgagcaga gccttttcca cacagcgtaa
    27361 tttttccgct atgcaaaaaa cgcatagcca gaaacatact tgcattttcc ccatacccac
    27421 atggctgata aggtttttcc gctccaggaa ttttcctggc tcaagcaaca cacaaacaca
    27481 aaaaaacaat ggggattgtt atgacacaac aaggggatgc tgtagccggg gagctggcga
    27541 cagaaaaagt gggcattaaa gggtatctgg ctttttttct caccataatc tttttttccg
    27601 gtgttttttc cggaacagac agctggtggc gcgtttttga tttttccgtt ctgaacggat
    27661 cgtttgggca actgcctggt gcgaacggag ccaccacctc atttcgcggg gcgggtggcg
    27721 cgggggctaa agatggcttt ctctttgcgc tggagctggc accgtcagtc attctctctc
    27781 tggggattat ttcaattacc gatggtcttg gtggattacg cgccgcgcag cagttaatga
    27841 cacctgtgct aaaaccgttg ttgggcattc cagggatctg ctcgctggcg ctgatcgcta
    27901 acctgcaaaa cacggatgcc gccgccggta tgaccaaaga gctggcgcag gaaggtgaaa
    27961 ttaccgaacg cgataaagtg attttcgccg catatcaaac cagcggtagc gccatcatca
    28021 ccaactactt ctcttccggc gtcgcggtgt ttgcctttct gggcacgtcc gtaatcgtac
    28081 ctttagccgt catcctggtg tttaaatttg tcggagccaa cattttgcgc gtctggctta
    28141 acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc atgacaactc aggtccgcaa
    28201 aaacgtcatg gatatgttta tcgatggcgc gcgccgtggc tttactatcg ccacgaccaa
    28261 cctactgcca aacgtggtga tggcgtttgt catcattcag gcgctgaaaa ttaccggcct
    28321 gctcgactgg gtggggcata tctgtgaacc cgtaatggcg ctgtggggat tacccggtga
    28381 agcggcaacc gtccttttag ccgcattaat gagtatgggc ggcgcagtcg gtgttgccgc
    28441 cagtctggcg accgccggca cattaaccgg tcacgatgtc accgttttac tgcccgcgat
    28501 gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc ctggggacag ccgaagtgaa
    28561 tgccaaatat tacccgcata tcattacggt ctgcgtgatt aacgcattgc tgtcgatctg
    28621 ggttatgcag cttattgttt aacaaggagt taccatgatt gattataccg cagccggttt
    28681 taccctgctg cagggagcgc atttgtatgc gccggaagat cggggaattt gcgatgtcct
    28741 cgtcgctaac ggcaaaatta tcgccgttgc cagcaatatc ccttctgaca ttgtaccgga
    28801 ctgcacggtt gtcgatctca gcgggcagat cctctgtcct ggttttattg atcaacacgt
    28861 ccatttgatt ggtggtggcg gcgaagcagg tcccacgacg cgcacgccag aagtggcgct
    28921 aagtcgcctg acggaagcgg gcgtcacgtc agtggttggt ctgctgggca ccgactctat
    28981 ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg ctcaatgaag aaggcatcag
    29041 cgcctggatg ctgaccggcg cttatcatgt cccttcccgc accattacag gttccgtgga
    29101 aaaagacgtg gcgattatcg atcgtgtgat tggcgtgaaa tgcgccatct ctgatcaccg
    29161 ttctgccgca ccggacgttt atcacctggc taatatggcg gcggaatccc gcgttggcgg
    29221 tttgctcggc ggtaaacctg gcgtcaccgt gttccacatg ggcgacagca aaaaggcgtt
    29281 acagcctgtc tatgacctgc tggaaaactg cgatgtgccg atcagcaagt tgctgccgac
    29341 ccacgttaac cgcaacgttc cgttgtttga gcaggcgctg gagttcgcgc gcaaaggcgg
    29401 caccatcgat atcaccagca gcattgacga accggtcgcc cctgccgaag gtattgcccg
    29461 cgccgttcag gcgggtattc cgctggcacg cgtcaccctc agctccgacg gcaacggtag
    29521 ccagccgttc ttcgatgacg aagggaattt aacccatatc ggtgttgccg gttttgaaac
    29581 attgctggaa accgtgcagg tgctggtcaa agactatgat ttcagtatca gcgatgccct
    29641 gcgcccgctc accagtagcg tagccggttt ccttaacctg accgggaaag gcgaaattct
    29701 gccaggcaat gatgctgact tgctggtcat gacgccagaa ctgcgcattg agcaggtata
    29761 cgctcgcggc aaactgatgg tcaaagacgg caaagcctgc gtgaaaggaa cgtttgaaac
    29821 ggcttaaacg gttgtcagcc gcccgaatgg acgtagaatg cccgcaaggg cggctgacag
    29881 agtaaaacgt aatggatgac tgtggtgcga ttttgcataa tattgaaacc aaatggcttt
    29941 atgattttct gaccctggaa aaatgccgca atttttccca ggcggcagtc agtcgcaacg
    30001 tctcgcaacc ggcattcagc cgccgcatcc gtgcgctgga acaggcgatt ggtgttgaat
    30061 tgtttaaccg ccaggtaacg ccgctgcaac tctcagaaca agggaaaatc ttccattcgc
    30121 agatccgcca tctgttgcaa cagttagaga gcaatctggc agagctgcgt ggcggcagcg
    30181 attacgcgca acgaaaaatc aagatagccg ctgcacactc tctttccctc gggctgttac
    30241 cgtccattat cagccagatg ccgccgctct ttacctgggc aattgaagct attgatgtcg
    30301 atgaagcggt cgataaactg cgtgaagggc aaagtgactg tattttttcc tttcacgacg
    30361 aagatttgct ggaagcgccg tttgaccaca ttcgcttatt tgaatctcaa ttgttccctg
    30421 tctgcgccag tgacgaacac ggagaagcac tttttaacct cgcgcagcca cactttccgt
    30481 tactgaatta cagccgcaac tcctacatgg ggcgattgat taatcgcacc ctgacgcgcc
    30541 acagtgagtt aagtttcagc accttttttg tctcttcgat gagcgagctt ttaaagcagg
    30601 ttgccctcga cggctgtggg attgcctggc tgccggagta cgccatccaa caagaaattc
    30661 gcagcgggaa gctcgttgta ctgaatcggg acgaactggt gatcccgatt caggcctatg
    30721 cataccgaat gaatacccgc atgaatccag ttgccgaacg cttctggcgt gaactgcgcg
    30781 agctggagat tgtacttagc tgacattctc cagcgtcgca ctgcgccaac cgccattatc
    30841 ccagaccaga tcaaacgtta gcggttcatc gtcgtggtac agcattatct caaggctgat
    30901 cgccagcgtg tgactgtacg cttctttcag attgacctgc aacgccgcaa tccgtggctc
    30961 atgaaataac agtgcagaac gcagctgacg gcaataccag ctctggagat gtttgtcgct
    31021 ctggttatgc acttgccact cgggcatgcc tgaaacaagt aatgacggta atgccgaaag
    31081 aggagaatta aaaagagtga ggagatcgct gcataccgat ttacgcagca atgacgtttt
    31141 attgcctgaa atttcaggta aaacagcctg aagtgattgg cgcatcattt tgcgcactcc
    31201 tgacgtttag caacatatgc ggcttgccct gaacaccggg cgccactgta ttgatgctca
    31261 ttttccatag ccattccctt ttgttcatta catccatgtc agtctgagac aggcctgaat
    31321 cctgtcagca aagtataaaa ccttacttgc ctatgaatat ctactgactt ttcaggaaaa
    31381 aagtcatgat ttgataaaaa tcatcaaact caaaaaaaaa ccgctagtat attctgaata
    31441 ttccgaatag ttacgtagat gaaaatactt gtcaccgccg ctacttaatt ccggtgggaa
    31501 attatttatt tccgcataat catccatgta ttatttgaaa caatcagttt gtaaataatg
    31561 taaattgata acatatcaaa tacatcaaaa ataccattca caggcattat taatatagac
    31621 ctcaatatag caacatcaaa ttcaaattag taataagata tgcttttaaa ttgcatgcaa
    31681 ataattttaa atgagcgttt tttgatactt tagagtgttt cctaccattt aatatcactg
    31741 tgtagagttg atatcactac aacagaaaca tgcatgtcat ttgtttactt ctcttttact
    31801 acagaaaaat ttatctattt ttctaaacat cagtgaaata ctcataaaag gtaactatta
    31861 tgtctatgcc attaagcaat gccgctaata accaattcta tataaataat cacttccttc
    31921 atcatccaaa agtcgacaat gagttaacac gtaaatatga acgtgcacgc ctggatacgg
    31981 aaaatatcta tcttctgcct cttactcgtg gcaataacca taattacgat ggaaaaagcg
    32041 ttgtcgaaat tagaaaactt gatattagta aagagtcctg gccatttaat tatgtgacag
    32101 aaacatgcag agaatccgat ggtattacaa caacaggtcg tatgctctat cgaaacttgc
    32161 gaattacctc agctttagac gaaatatatg gtggtatatg taaaaaggcg catgctgcca
    32221 cagagttagc agaaggatta cggctaaatt tatttatgaa gagcccttcc tatcctgttg
    32281 aggactatac agttcatgaa ataacattag gccttggttg caatgttcca ggttatgccg
    32341 gaaccacgat aggatatatt tcaacccttc cagcttctca ggcaaaaaga tggaccaatg
    32401 agcagccaag gattgatatc tatattgatc agataatgac ggtaacaggg gtagcaaatt
    32461 cttccggatt cgcccttgcc gcattactta atgctaatat tggaatggga aatgacccaa
    32521 taattggtat agaagcctat cccggtaccg cggagatcca tgcaaaaatg ggatataacg
    32581 ttatccccgg tgatgaagat gcgccactca aacgcatgac tctgcaaccg tcatctcttc
    32641 cagaactgtt tgaattaaaa aatggtgaat ggaattatat tggtaaatag ccactgagaa
    32701 gaatgagtac taatattcag gttatacaga tatttaaaca ataccctgca atttacaaag
    32761 taatactcat tgcgacttct taatttaaga aagcgcaagg aacgtttacc cttgcgctta
    32821 ttataataag tcaggcgtcg actttatcca gcggccatga atcaaacaga tagccgtcaa
    32881 aataaatatc gtcaacgttc gagaattcca gcagacgttg cttaacgttt tccagatgct
    32941 gccacattgc cagcttcgcc gctcgggcat cttttttgat taacgcggcg aggatctgtt
    33001 tgtgatcgcc caaccactct ttgcgataca ggctgtcatc cagatggctg tgcaactgga
    33061 tccacattgg gttgttttcc cgccactgcc aggactgacg gaacagctcc accagcatgc
    33121 tgttatgcgt tgcttcggca atagcgagat ggaactgcat gtcaccgctt tcgctgctgc
    33181 ccggcgcact ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc attttgacga
    33241 tatcttcgcg ggtagcctgc agggcggcaa actcggcgat gttactttcc agtaactgac
    33301 gcgcctgtaa cagctcaaaa ggaccggcat cgttgcagac gttggcatcc ggactgtctg
    33361 tgttctggct gcctgagtta tcaagaacat agataccggc accccggcgt acttccacca
    33421 gccctttgat ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg acatcaagca
    33481 tttctgcaat ttcacgctcc ggcggcagcc gttcgccagg attgtacggc gtctttatga
    33541 tcagatcgcg gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag gtggcagatt
    33601 tcatggtgtt gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg
    33661 ccttatccgg cctacaacgg gcaattttac ggcccccgtt aatctcaaac tataccatta
    33721 gcactggaag ttggcacgta caggtgggca aaaaaacgaa aactggtcaa ccaaatatca
    33781 gtttgattga ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc cacacactcg
    33841 cgcgcaccgc gttcgcacag ctgctgatat gccgccgtca ctgcgccaac aaagtcggca
    33901 ttctgcggca gatcatcggc aaaaataccg ctcaggccca gcagcgcttt cacgcggtct
    33961 gcgccctgat actgcgcgtt gaccttctgg aactccgcca gcatcgggtc gaccacgtca
    34021 atggcattac cctgctcatc cacgccctgg gtgtaacgca tccagccagc cacgcccagc
    34081 gccaggtgac gccagctgcc gccgttttgc aggtgcagac gcaccgggtc cagcagacgc
    34141 tgcggtaact tctggctgcc gtccatcgca atctgccagg tacggtgacg cagagacggg
    34201 ttgctgaaac gctcgatcag cagcgtcgca taggcgttca ggtctgtacc ttccggcatc
    34261 gacagcgttg gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg ataagccggg
    34321 ttagtcatgg tgtcggcaat ggtttcatag ccgccgaggt aaccgaggta cgccagaaaa
    34381 gagtggctgc cgttcagcat acgcagcttc atcatttcga acggcacaac gtctgcaacg
    34441 aactgtgcgc ccactttatc ccagtccggg cgaccattaa cgaagttatc ttcaatcacc
    34501 cactgacgga acggttcgca ggcaatggcg cacgggtcgt aaacacccag ctggtcagca
    34561 atttcctgta aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg
    34621 aaggtgacat tttcttcaat ccatgccgcc agctgcgggt cacgcgcctg agccagcccc
    34681 agtaccgcga ccttcgccac atgaccgttt tcacgcacgt tatcgcagga catcaccgta
    34741 aacgctttca accctttttc acgacgcaga cgcaaggctt cgacgatgta accaatcgcg
    34801 gacttcggcg cagtcgggtt ttccagatcg tgcttgatca gcgggttatt gagatccagc
    34861 tggccgctcg ccgcatcggc gcagtaacct ttttccgtga ccgtcaggga gacaatcgcc
    34921 gtttgcggac gcgccatcgc gttgagaata ccttcgcagc cgtcgatttc cggatgcagc
    34981 gcttctttca tcgaaccgat aattttcagc tcggtgcttt ctgcgccttt ttccgctacg
    35041 gtgtacagca gttgctgttt tttcaggttt tcgatcagca cgcggtcgtt gcctggcatc
    35101 aggttaactt cgcagatgcc ccagtcgctg tcggtgcttt ccagcagatg atgggtatac
    35161 agcgcctggt gcgcgcggtg aaacgccccg caaccgagat gcacaatgcg tgattccaga
    35221 cgagaatgat cccatgacgg gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc
    35281 gtaaactcca gaatccgtct tcagagagtg acggcaagga agaaggaacc ggcacggcgc
    35341 gcagccatgc cggtggatat taacggctaa agaaagcgcg ctggatcgcc agttcgacac
    35401 cgcgaacttc ggccaggcct ttcagacgac caattgcgga gtaacctggg ttggttttct
    35461 tcttcaggtc gtccagcatc tgatgaccgt ggtccggacg catcgggatc aggtcttctt
    35521 tgccttccgc tttacgacgg tgttcttctt caacaatcgc tttcaccact tcgtacatat
    35581 caacgtcacc gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg
    35641 tggagcgcag atgggtgaag taaatacgcg gaccgaactg cttgatcata tcaaccagat
    35701 cgttgtcagc acgcacgccg taggaaccgg tgcacatggt gaaaccgttc gccatgctgt
    35761 ttacggtatc aaccatccac tgcatatctt caatggtgga aacaatgcgc ggcaggccga
    35821 ggatcgggcg cggcggatcg tccgggtgaa cagccatacg cacgccaact tcttcggcaa
    35881 ccggaataat cgctttcagg aagacggcaa agttttcgcg cagtttagct ttgtcgatat
    35941 ctttgtaaag ctccaggtgc ttacggaact ggtcgagggt atacccttct tccgcgcccg
    36001 gcagaccggc aatgatgtta cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga
    36061 agcgaacagc agcctgagca atttcttctt cggtgtaatc cgcttccgcg cctggacgtt
    36121 tcaggatatg catttcgaat gcagcgaatt cgatctggtc gaagcgcaga gctttggagc
    36181 cgtctggcag cacgtattcg aggtcagtac gggtccagtc gagcaccggc atgaagttgt
    36241 agcacacggt gcgaatgccg cactgcgcca ggttgcgcag agtctgctga tagttagcga
    36301 tccactgctc atagttgcca gtgtgggttt tgatatcttc gtgaattggt acgctttcta
    36361 cgacagacca caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta
    36421 cggaccatac ttcgccgttc gggatatggt gcagcgcggt aaccacgcca gttgcgcccg
    36481 cctgacggac atcagctaaa gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt
    36541 ccatgatttt tcctctttca catagtgggt tccacagcaa agagacgcgg aaccctggag
    36601 ttgtttaggt atccggatga acaaccagac cgcagccgtg gcgttgcatc ggatgagatg
    36661 gcgtataagt tctacctttt ggtaagacat gtcaacccga attctgaaat tggttaacca
    36721 catcacaaga atttcactct catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg
    36781 gttattgatc agaggatgtg acattcatcg caacaatggt tgaccaattt gcataacatg
    36841 tcgcccaaaa taacaacggt tcaaccaggt aaggtaaaac gttatgcatg tgcttaacat
    36901 tctctgggta gtattcggta tcggcctgat gctggtactg aatttgaagt tcaaaatcaa
    36961 ttcaatggtg gctttgttgg tggcggcgct gtccgtcggg atgctggcgg gcatggattt
    37021 gatgtcgctg ctgcacacta tgaaagcggg cttcggcaac acgctggggg aactggctat
    37081 catcgtggtg ttcggtgcgg tcatcggtaa attgatggtc gactccggcg cggctcacca
    37141 gatagcgcat acgctgctgg cgcgtctcgg tctgcgctat gtacagctgt cggtgattat
    37201 catcggcttg attttcggtc tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc
    37261 gctggttatt gttattgccg ccgaagctaa aattccgttc ctgaaactgg cgatcccggc
    37321 agtagcagct gccactaccg cacattcact gttcccaccg cagccgggtc cggtggcgct
    37381 ggtgaatgct tatggcgcgg atatggggat ggtttatatc tatggcgtac tggtgacgat
    37441 cccaagtgta atctgcgcag gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc
    37501 aacgccatca ttcctgaaag cagatcaacc ggtagatatg aataatctgc cctctttcgg
    37561 cgtttcgatt ctggtgccgc tgatcccggc gatcattatg atctctacca ccatcgccaa
    37621 tatctggctg gtaaaagata cccctgcctg ggaagtggtt aactttatcg gttcctcgcc
    37681 gattgcaatg tttattgcga tggtggttgc attcgtactc tttggcaccg cacgtggtca
    37741 tgacatgcag tgggtgatga acgcttttga aagcgcggtg aagagtattg caatggtgat
    37801 tctgatcatc ggtgcgggtg gcgtgctgaa gcagactatc atcgacaccg gcattggcga
    37861 caccatcggc atgttgatgt cccacggcaa tatctcgccc tacatcatgg catggctgat
    37921 cactgtgcta attcgtctgg cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg
    37981 gattataagt gctgcaatcc tagatccagc aactggtcag ctggttggcg tgaatccggc
    38041 gctgctggta ctggcgacgg ctgcgggttc caacaccctc acccacatta acgatgcatc
    38101 tttctggttg ttcaaaggtt actttgacct gtcagtaaaa gacacgttga aaacctgggg
    38161 tctgctggag ctggtcaact ccgtggttgg gctgattatt gtgctgatta ttagcatggt
    38221 agcgtaaaag gacaccagag cctgccaatg gcaggctctg actgatgaca aagtctaaaa
    38281 atgtgcccag acagtcctct cgcccctctg gggagagggt tagggtgagg ggaaaagacc
    38341 agcaaagtgc gctttgttta tgccagatgc gacgctaacg cgtcttatct ggcctacaaa
    38401 gggctaacgt gcaggttttt agcttcaggt aatattgcgt acctgcatta gcaatgccct
    38461 gtgatttctt tattgataaa caaaagtcac gccaataatc gattgcacat tccctgcagt
    38521 cacctgccct ccggtacgtg cgtaatttgc cgttaatccc agacttaccg ccgaagtccc
    38581 tactgctcct aacgataccg tgttattcgc tggaataatc gtaccgttgc gcgtcaactg
    38641 tacgccgacg ccctgcgctg gtgaaaacga cgcggtattg gtgaaaatcg agttgcccgc
    38701 atctgcggtt gtgccggaga ggtaataccc caggttttgg cttttcgcac aataaacggt
    38761 aagaggaatt ggcactgaac cagggtagtc cggcagagta acggtgacat cacgagcaga
    38821 aacatcgcag ccgccagtag gcaccaccac atcattattg gcgtaaatat tccacacaaa
    38881 ctggaaatca tcgctgttat agttgttggt ctgtcgcaaa ataagcacgg caattaatga
    38941 gccagcttta atcgccaccc cgcccgcact gctcacaggc gtcaaataaa gcgccaccgg
    39001 ccacggctta tccgttctcg aattataaac aacccgcggc gtttcgctgg tggtcggaaa
    39061 tggatagcta ctgccactat attttacggt cccggaaaaa ttagataaca cgccgccata
    39121 agccgagcct cgttgcagtg tgacatagtc tgtaatggtt tccggataat cgttatggca
    39181 aaagatttgc gtcgaaagat ctacgaccag gttttgcccc acattcacgg caggcgcaag
    39241 gtttacataa acattagcgc tgccaccgcc aatagggata gcggtaccat tggcggtttt
    39301 acaggcgaat gaccaggcat ttaccgacca gcccatcagc agtacagcaa acagggtaat
    39361 aactcgtttc attacaatca tctcttcggg ttcagctgta ggtataggtg atgctaatca
    39421 ctgcctgaat ggttccctga gtggctccgc catttaccgt caatactctg acctgtaacg
    39481 ggaagtgcgc tgattgtgag gaatcatcca cctgaactgt tttggttgcg ccagtattca
    39541 atgtgttgcc actgtcatcc tgtagctcta actggatgtt ttgcgcggtc ccctggtttt
    39601 tataatatcc ggtactgtcg gctgccccgc tgaagctggc agtggccctc gacgttccca
    39661 ccggacaatt agtcaactca agcgcaacat catgccaggc cgatgccgcc ccggcagaca
    39721 tcagactgaa agaataaaga tcgccgagat caaccatggc attggtggtg gaaactgtgc
    39781 acggtttggc gacgacctta ccgttcaccg tgatggtgac atcggctgcc tgtatcgtcg
    39841 cacttgcgag cgccaatatt gccgccaata catacccacg tttgcaccat ttcatgagca
    39901 tctccagtta ctgatattca agagtgaagg tagccgtggc attgatatgc cctgcagtga
    39961 caggcacctg tgtcgccatt agccgggcgt aaaaattcag cgtatttggt ttacccggcg
    40021 tcagggtcgt ccacgaaagc gcggacgatg gagcattaag gggtatttga ttttgctgct
    40081 cattcagaag ctgtattccc agtcccgaag ccgctgacac cgtattttca agtgcaagca
    40141 gattggcatt gtggctatct gcaacgccgg taaacccaac ttttacggca gaaacggcat
    40201 taccacaggg tgacagcaaa atacgaaatg gaacaacagg agtcgtcgcg ccaatgttgt
    40261 taaattgctt cgccgcgttt tccatcagat caacagtaaa attggttgat tcagcggcca
    40321 cactacagcc gttatccctg acatagccgc ggatagtaat cgtgctatcc gcagccaaag
    40381 cgtgactcac cgccagccac aaaaaagcgc acagaagata aaaaggtttg tttctcatca
    40441 cgccccctta acgacattca gctgatagct gggttaataa ctgctgctga ctctctggtg
    40501 gcagttgata attggcgaca cagtgagcat tttcctcttc tccccatttc acctgaactt
    40561 ttcccgctaa aggcattccg ctgaggtaaa cctgaccatt atccgcaaca atgccgctac
    40621 tctggctact ctctgatgtc accatcgccc caaacggcag cggcttatta ttgtgggtta
    40681 gcgtcatgag cagttttatc ccaacgcgcg ctttaaactc tgctcgcacg atcgccccac
    40741 gagtgggaac aacgttagcg accgcgttat ctaaatcgat gttatcagcc agggtattgg
    40801 tatccagcgc cactctattt tcccgatatt cagtggcata aggcagcacg gcataaccac
    40861 gccagtcggt acgcaccccc gtctggtttt cgacttttgc atcttttgcg ccaggcgctt
    40921 taacaagcac cactgtttcg tttaacggct gccccagcgt tacgccattg gcatgagcca
    40981 gtaccccacc gctgactccg taatagagct gcttaatatc atcgctatgg ctgtaaccga
    41041 tattggcatt gccgtaacca ccgcgataat tcagcgtggc gtagcctgta cttccgttat
    41101 ttccatcgcc tcccccggca tagccggttt gcacgctata gctgaggttg ttgtcttcca
    41161 gcaacgtacc gtatacacca gccagattgg tcatccgacc gttgagatcg tgtgacatgc
    41221 tgtagctggc actggcatgt cgccactgag atttactgtc agaacgcagc cagtggctga
    41281 aaggaatatt gacgttaagc gctaacatct gatcccgtcc tttttgccag gcgtttttcg
    41341 tcaggctata gctgagcgtc cagttgatat cttcgaatgc agtatttaat ccagcctgga
    41401 attgctcatc gacattactc gttccccaat aagtttgatg gctaccactc aaatacagtg
    41461 ttgatgtgcg cccgagttgc tgagtaacgg tgagctgtaa tttcccgcgt ttgttataag
    41521 cgaggttgta atagtcggtg aatttcggct taacctgaat aactccatcc tgtgtttcga
    41581 tgttgtagcc attcattcga ctgtatgttg tatcagcgaa attaaaatat ccgctggtcg
    41641 aataacggta acccactaac tgaatattcg tgcctgactc attgagcgat ttgttataga
    41701 gaaaacgcac cgattgtccg tcatgctgac tgtcatcggg aagtgtggaa ttagcctgcg
    41761 tcatatccac agacagagcg cccagtgccc ccatattttt cccgatacca aaattaaaag
    41821 cacgataacg atccgccagt tgcgttccac catatattgt ccagccagcc ggaaggccgt
    41881 ggagtaatgt actctggaaa aagcggggtt tttcctgctg cgcatttcca ctacggtatt
    41941 ctcctgccgt aatggaataa cgagtatgcc cttcacgttg caaaagcggg actgacgaat
    42001 agggtacggt aaaaatctgc gtgctgccgt cagcctcttt gatcgttacc tgcaagtcac
    42061 cactattacc tgcggcatag atatcgttga tggtaaaagg ccccggtggc accgtactat
    42121 tataaatgtc atacccattt tgtttaatag tgacctgtgc agtaccacga gcaataccgt
    42181 ggatcaccgg ggcaaatcct ctttggctat cgggtaacat attgtcatct gaggccaatt
    42241 gtgcgccgcg aaagttaata ccatcgaaaa tatcgccctg ggtataacca tcacccagcg
    42301 tcagccggga acgtaacggt attatgtctc gctcaagcca ggtattgata tgctgccatt
    42361 tatttttgct acctgatgaa ctgtcgctac tgttataact ccaggtggta ttgtcgcgta
    42421 aacgccacgc accaatattt aacccactct gtaggtttaa atatgcataa tggctgttac
    42481 ccccaatccg attctgtaca ctatttccgc tgaaattata attgagcaat ccggcattaa
    42541 taccgggatc ccataactca ggaggaatat aaccacgcgc gcgattactc ataaatgcct
    42601 gagggatcgt caggttgagt cgctgctgac caacatctaa atgcgcagta gcgtcatgga
    42661 tcattgaggt taatggcacg caggcatcat ccgccagcag attcataccg gagacagaag
    42721 ccgtattcag ccccatactg gcaagttgcg cgcgtgtcag gcagggaaca atcccttgtt
    42781 cgctgtcgcc cgtattaaat gtgacatcac gcgttgccat ataaccatta ttcaaataga
    42841 tatcgacacg atacgtccct ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg
    42901 ccacagcctg gggatcatcc gctaaaaagc gcggattaaa atagagttcg gcagatgaca
    42961 aaggtgcctg tgcggcaaaa gcacaggcga caacgagccg gacaaaaaaa ccagccaaac
    43021 gatgcttacg aatatgcaag cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg
    43081 acattattcc tccggtttaa aatcacccgg cagtcaattc tttttattca ggcgaaaaat
    43141 tccccctgcg ttattccatt acgcccgtca tttttggggt aagtgcacca taatcattta
    43201 ttgttcggta agtaatattg cttcccgcat cagaaggcaa tttaaccgtg ctttcgccca
    43261 ttggaggcac caaggcattt tcaagaaccc gggttccggc attcaactct gttaccgtca
    43321 ggtaataggg tgtcgggtta atcagcgtca gagaattcgc gctacgacga aatcttaatt
    43381 tttctgcggc ctgatcgggt ggcaacgcta atttagccgg gcgatagtac agtttaatgc
    43441 ggctgataat tgcgagctgt agcgtattct cagtcaattt tgatttatcc attgacggaa
    43501 tcgctttaac attcatccag aataaacttt cccgatcctg tggcaattgg ttatttgttg
    43561 catcaagaat acgcaaggta ttctctcttt ttcccttcaa cgcaaacaga ggaggcgtca
    43621 cgataaaacg accatccttt acaccatcgg cattttccac ccatgattga attaaatagg
    43681 tactattttc atcattattt gtcacggcaa gttgcacttg tttttgccct gccggataaa
    43741 ttacgcgagt cgcacctaag gccactcccg cttcagcgcg tccggcaacc atcattgcca
    43801 tgaacatcag gatacctgcc agcaagcaga atgttatttc ctgcgatttc cttacattga
    43861 cgtttttatt actcactgtc ctgttcctgt tatcacatta tctgctgaac aattactgat
    43921 aggttaaaga gaaccaggcc tgggcattgg cgatgccgcc agtaacccga cgcccggtag
    43981 cacgatattt ggcgatgaaa tgtagcgaag tagagcctga gtaaagccgt ttccagtttg
    44041 ctggaggacg attaatcggt acgaggtttc cttcatcatc aaacaacgct acgccaatat
    44101 tggtggctat ccctggcccc tctcccacgg aaagcacatc cggattttta ccatccgcga
    44161 caccgtgaaa cgccacacct acacgttcgc tcaccaccgt gctacattcc cgtaaatgaa
    44221 taacaaaagg caccggtgcg ctatcttccc ctaccgcatg aaaccggtta ctgctgattt
    44281 gccccatatt gaccgtcatt tgtttatcac cggcttcaat ccggcaagtt tccgcaataa
    44341 tgacgccctg aaattgcata tttccgccgg gcagcgtggt attccattta tttccggcca
    44401 gagcaaacat tggcagcaac gacgctaata gaaataatct cttccttttc atcgttattt
    44461 ttatcgcaca agggtgggca tccctgcccg taatgacgtc cctgaacctg ggtaggttat
    44521 tgatactgaa ccttgaaggt cgcatccgca ttagctgcgc caggggttgc ggcaccggtt
    44581 gcaaaataac gcgcctggaa cggaatggtg ttggttccgt tattcagggt tgtttctgaa
    44641 ctaaatgtcg caccgtccag cgccagcgca gcacccgttc tgtccaggat ctgcacacca
    44701 acgtttgttg cgctacccgc agctgaactc tgcagagcta atacgttagg atgagtgcca
    44761 tcaattgcgg taccaaggaa ggcaatagct gctttgtctg caacagtagt gtcacaatcg
    44821 ttcagttgaa tgttaaaacc gacagcagaa ctggttgctc cttcttgtgc cagcgatgcg
    44881 gtacgaacct gtcctaactg aacggtttga tcaacagagc ctgcatcaac tgcgcaagcg
    44941 gcattaacaa cttccccttt aaagtgaacg gtcccaccat ttaccgtcgt agtatcggcc
    45001 agagccgctg cggaactgag ggacagagcc gacagaacaa cgattgccag agttttaatt
    45061 ttcatgctgc tttcctttaa aaaaactatt tctaaatcga catgggcagt cgttctgtac
    45121 actttgtttt gtcaacgagt ttgactgcca acactgcaca gttttccccc aaaagatgaa
    45181 acatttgggg ccattttgac tcatagagga aagcatcgcg cacaaacttt ttcagtttat
    45241 ttgttggctt aatattctat tattatcttt atttatagat ttttatattg catgaggtgg
    45301 tttttggaga gaagaatgag gaagatgcgt cgagccacag aaacgttagc tttacatata
    45361 gcggaggtga tgtgaaatta atttacaaga gaaataattt acatatcaaa cagttagatg
    45421 ctttttgtcg ttttttaata tttttatgct tgagaaaaaa tacgtaactt atttatgata
    45481 tggacagttt ggccccaatt gtcttgtatt tatttgtttt
//
LOCUS       DAEVZA010000024        44079 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEVZA010000024 DAEVZA010000000
VERSION     DAEVZA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.024"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..513)
                     /gene="djlC"
                     /locus_tag="LAF92_001378"
     CDS             complement(<1..513)
                     /gene="djlC"
                     /locus_tag="LAF92_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBI9790415.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVGEADNEDKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSL"
     gene            complement(510..1217)
                     /locus_tag="LAF92_001379"
     CDS             complement(510..1217)
                     /locus_tag="LAF92_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI9790416.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            1319..1873
                     /locus_tag="LAF92_001380"
     CDS             1319..1873
                     /locus_tag="LAF92_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI9790417.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(1883..3310)
                     /locus_tag="LAF92_001381"
     CDS             complement(1883..3310)
                     /locus_tag="LAF92_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBI9790418.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDKGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(3307..4014)
                     /locus_tag="LAF92_001382"
     CDS             complement(3307..4014)
                     /locus_tag="LAF92_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI9790419.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            4178..5155
                     /locus_tag="LAF92_001383"
     CDS             4178..5155
                     /locus_tag="LAF92_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBI9790420.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(5225..5707)
                     /locus_tag="LAF92_001384"
     CDS             complement(5225..5707)
                     /locus_tag="LAF92_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBI9790421.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            5942..8524
                     /gene="leuS"
                     /locus_tag="LAF92_001385"
     CDS             5942..8524
                     /gene="leuS"
                     /locus_tag="LAF92_001385"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBI9790422.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            8539..9120
                     /gene="lptE"
                     /locus_tag="LAF92_001386"
     CDS             8539..9120
                     /gene="lptE"
                     /locus_tag="LAF92_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBI9790423.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            9120..10151
                     /gene="holA"
                     /locus_tag="LAF92_001387"
     CDS             9120..10151
                     /gene="holA"
                     /locus_tag="LAF92_001387"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBI9790424.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            10153..10794
                     /gene="nadD"
                     /locus_tag="LAF92_001388"
     CDS             10153..10794
                     /gene="nadD"
                     /locus_tag="LAF92_001388"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBI9790425.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            10818..11429
                     /locus_tag="LAF92_001389"
     CDS             10818..11429
                     /locus_tag="LAF92_001389"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBI9790426.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            11689..12006
                     /gene="rsfS"
                     /locus_tag="LAF92_001390"
                     /gene_synonym="rsfA"
     CDS             11689..12006
                     /gene="rsfS"
                     /locus_tag="LAF92_001390"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBI9790427.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            12010..12477
                     /gene="rlmH"
                     /locus_tag="LAF92_001391"
     CDS             12010..12477
                     /gene="rlmH"
                     /locus_tag="LAF92_001391"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBI9790428.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            12508..14409
                     /gene="mrdA"
                     /locus_tag="LAF92_001392"
     CDS             12508..14409
                     /gene="mrdA"
                     /locus_tag="LAF92_001392"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBI9790429.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            14412..15524
                     /gene="mrdB"
                     /locus_tag="LAF92_001393"
                     /gene_synonym="rodA"
     CDS             14412..15524
                     /gene="mrdB"
                     /locus_tag="LAF92_001393"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBI9790430.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            15535..16623
                     /gene="rlpA"
                     /locus_tag="LAF92_001394"
     CDS             15535..16623
                     /gene="rlpA"
                     /locus_tag="LAF92_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBI9790431.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            16763..17974
                     /gene="dacA"
                     /locus_tag="LAF92_001395"
     CDS             16763..17974
                     /gene="dacA"
                     /locus_tag="LAF92_001395"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBI9790432.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            18085..18348
                     /locus_tag="LAF92_001396"
     CDS             18085..18348
                     /locus_tag="LAF92_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBI9790433.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            18449..19090
                     /gene="lipB"
                     /locus_tag="LAF92_001397"
     CDS             18449..19090
                     /gene="lipB"
                     /locus_tag="LAF92_001397"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBI9790434.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            19349..20302
                     /locus_tag="LAF92_001398"
     CDS             19349..20302
                     /locus_tag="LAF92_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBI9790435.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYITQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            20511..21476
                     /gene="lipA"
                     /locus_tag="LAF92_001399"
     CDS             20511..21476
                     /gene="lipA"
                     /locus_tag="LAF92_001399"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBI9790436.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(21577..21780)
                     /gene="tatE"
                     /locus_tag="LAF92_001400"
     CDS             complement(21577..21780)
                     /gene="tatE"
                     /locus_tag="LAF92_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBI9790437.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(21909..22697)
                     /locus_tag="LAF92_001401"
     CDS             complement(21909..22697)
                     /locus_tag="LAF92_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBI9790438.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            22790..23173
                     /gene="crcB"
                     /locus_tag="LAF92_001402"
     CDS             22790..23173
                     /gene="crcB"
                     /locus_tag="LAF92_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBI9790439.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(23227..23436)
                     /gene="cspE"
                     /locus_tag="LAF92_001403"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(23227..23436)
                     /gene="cspE"
                     /locus_tag="LAF92_001403"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBI9790440.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(23611..24171)
                     /gene="pagP"
                     /locus_tag="LAF92_001404"
     CDS             complement(23611..24171)
                     /gene="pagP"
                     /locus_tag="LAF92_001404"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBI9790441.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            24760..26145
                     /gene="dcuC"
                     /locus_tag="LAF92_001405"
     CDS             24760..26145
                     /gene="dcuC"
                     /locus_tag="LAF92_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBI9790442.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(26186..26866)
                     /gene="dpiA"
                     /locus_tag="LAF92_001406"
                     /gene_synonym="citB"
     CDS             complement(26186..26866)
                     /gene="dpiA"
                     /locus_tag="LAF92_001406"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBI9790443.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(26835..28493)
                     /gene="dpiB"
                     /locus_tag="LAF92_001407"
     CDS             complement(26835..28493)
                     /gene="dpiB"
                     /locus_tag="LAF92_001407"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBI9790444.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            28873..29931
                     /gene="citC"
                     /locus_tag="LAF92_001408"
     CDS             28873..29931
                     /gene="citC"
                     /locus_tag="LAF92_001408"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBI9790445.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            29946..30242
                     /gene="citD"
                     /locus_tag="LAF92_001409"
     CDS             29946..30242
                     /gene="citD"
                     /locus_tag="LAF92_001409"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBI9790446.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            30239..31147
                     /gene="citE"
                     /locus_tag="LAF92_001410"
     CDS             30239..31147
                     /gene="citE"
                     /locus_tag="LAF92_001410"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBI9790447.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            31158..32690
                     /gene="citF"
                     /locus_tag="LAF92_001411"
     CDS             31158..32690
                     /gene="citF"
                     /locus_tag="LAF92_001411"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBI9790448.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            32694..33245
                     /gene="citX"
                     /locus_tag="LAF92_001412"
     CDS             32694..33245
                     /gene="citX"
                     /locus_tag="LAF92_001412"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBI9790449.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            33220..34098
                     /gene="citG"
                     /locus_tag="LAF92_001413"
     CDS             33220..34098
                     /gene="citG"
                     /locus_tag="LAF92_001413"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBI9790450.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            34149..35612
                     /gene="citT"
                     /locus_tag="LAF92_001414"
     CDS             34149..35612
                     /gene="citT"
                     /locus_tag="LAF92_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBI9790451.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            35726..36532
                     /gene="rna"
                     /locus_tag="LAF92_001415"
     CDS             35726..36532
                     /gene="rna"
                     /locus_tag="LAF92_001415"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBI9790452.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            36762..37172
                     /gene="rnk"
                     /locus_tag="LAF92_001416"
     CDS             36762..37172
                     /gene="rnk"
                     /locus_tag="LAF92_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBI9790453.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(37403..38641)
                     /locus_tag="LAF92_001417"
     CDS             complement(37403..38641)
                     /locus_tag="LAF92_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HBI9790454.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            38862..39290
                     /gene="uspG"
                     /locus_tag="LAF92_001418"
     CDS             38862..39290
                     /gene="uspG"
                     /locus_tag="LAF92_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBI9790455.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(39411..40976)
                     /gene="ahpF"
                     /locus_tag="LAF92_001419"
     CDS             complement(39411..40976)
                     /gene="ahpF"
                     /locus_tag="LAF92_001419"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBI9790456.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIKTASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(41105..41668)
                     /gene="ahpC"
                     /locus_tag="LAF92_001420"
     CDS             complement(41105..41668)
                     /gene="ahpC"
                     /locus_tag="LAF92_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBI9790457.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            42040..42786
                     /gene="dsbG"
                     /locus_tag="LAF92_001421"
     CDS             42040..42786
                     /gene="dsbG"
                     /locus_tag="LAF92_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBI9790458.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            42995..43897
                     /locus_tag="LAF92_001422"
     CDS             42995..43897
                     /locus_tag="LAF92_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9790459.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
BASE COUNT        11109 a        11180 c        10720 g        11070 t
ORIGIN      
        1 aaggctaagg aaatcgaaac aatcaccagc ctgaatggcc tcaagaaaag cctccctttc
       61 ttcctcatcc attccttcgc tgtcattaaa cgaatgccag ttcaaacgct ctgccagcaa
      121 agaagcacaa tttaatgaaa gatatcgcgc ttctatagcg attgcacaca gcggccagcg
      181 taattgatcg acatcctcca tgttccaggt atttaattgc tgaataaatt tctgccaggc
      241 ggagggctga aaacgatcac tttctgaatc cagtaatgtc ctgaatgcac gtagtatttc
      301 atgttcagcg gcggcatctt tatcttcatt atctgcttcc ccgacaggat ttaccgccag
      361 tcgcagggcc tcttcatagg cctggcgtaa tgctttaaac ccctgcggat cggtttcggg
      421 atgacataac ggtaagcgag caagataagc ctggcggata atgtctattt gcgtcgtgct
      481 ttcaatttcg aggatttgcc aacatgtttt cataggtcgc tcacatcctt aagcgttacc
      541 gggcattcag gctggacagt taatggctgc cagggaagcg tggcatagaa acgatcgctt
      601 cccgctgcca gctcttcgaa catccgactc ccggcgctat cgtatttgta cagggttagc
      661 gcctcgcgta gcgaagtgtt gtcttcgcac tgagaagatt gccagtagag acgtcctaac
      721 gaataggcgg aaaaatagtg catccagctg tgatagtaat ggtgagctcg tacatagacg
      781 cgcgattgca gccataaact ctcttcttcc gtcagccagt tgttgagcac accgacccgt
      841 gacagaaacc ccattcgaac ataatcccag gctttgattc ccccttctcc acagcacata
      901 gcggtttggg caacgaattg ggtataaatc aaccctctct ccgagccacc agcaattaat
      961 gctttccact cttcagggga agagcgatgc catgtcagat ataagccagc taaatcattg
     1021 gcatgaccat catcagtcat ctgataaata gtctgaatta aatcatcacg attatttatt
     1081 ccccaggatg atgagagatc gaattctttt ataccggtat acatttttgg cgagtcatgc
     1141 gcgggtttgc aaccctgatt aagtatttcc atgggcgcag aaagcgcaaa aagaagatat
     1201 tgttcttcct tattcatttg ttaataatcc cttaagtgca ttaactgact acttttttat
     1261 tattgatgag tataaacttg acgctttcaa aataaaaaga aaatcgaagc attcacacat
     1321 gaataaaaaa ttaatgtata tattcgcaat ttttatagtt gcagcaatta cctgtattag
     1381 ccaacccaag aaaacgacgt tgcgtgataa agccatggtg aattatgcct ttgattattt
     1441 aagatcaccg ggcagtcttc cattcaccac ggcagccacg gagctttccg cgattcatgg
     1501 tcactcaacg tcgcaatatc gccttggaga attttatctt catggtagcg acggtaaacc
     1561 actggattat acacaggcga gatactggta tgagcaatca gcggaacagg aaaatccacg
     1621 cgcgcaaagt aaactggggt ggatctacct caaaggtctg ggggtcaaac ccgacacccg
     1681 taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg ctcaatatac
     1741 tttaggtttg atctacagaa atggctcagg tattaatgtt aaccattatg aatctcaaaa
     1801 atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac ttgccgggct
     1861 tcccgcacat taacgtcgac tgtcacccga acagatggag tattttccct atcaaaccag
     1921 caaagataaa aatatagaaa ataattttaa tgatgcccag acagcctggc ccaccatcgt
     1981 caacgggcat tggctgcggt tgcggctccg gttcaacagt ttgcttctcg gtaaaattcg
     2041 ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga tcttccgcat
     2101 caaggcggct cacaatcggc attaatgatt cttctgtcgg ttggctagcc tgcactaatt
     2161 gtttggcaac taaatataat ggccataagg gatgttctgt atcacgcgtg agtgagatcc
     2221 gcggcttacg tcctttgtgc aaaaccatct cgaccagtga acgtcccagc gcagagagtt
     2281 tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg ccggggacat
     2341 aaagtagcgg ttttccctta tcgttaacca gatcacgcgc ttcaatcatc atcaccacta
     2401 atgggtaata atcgacccga tgctgacggc accattgcaa cattaaagca tccgcctggg
     2461 tggagggata ctcgcgccag tagtcacaaa gttctgccag caagctttcg ccttctccac
     2521 aatagacgcg ttgcgcgtat tcataataac gcgcatcttc attatcaggt tcagcttccc
     2581 gccacgaccg ggcgtaatcc agaagttcgg ggatatccag gcgacctgcg ctataccagc
     2641 ggagtaaatt tctgtgcagt tttgcatcat cggggatgat cactgcgcga tgttgcgcta
     2701 aatacaatgt cagccattct ggatgcgatg accaaatccg agcgaacatt tggtaaaaat
     2761 cgatggtgtc attttgcacg gcaacgggca gatgcagaat ggtctggaag ttaaaaacat
     2821 tccccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt tccccgacgt
     2881 tttcctcctg caaccaccgc aatctttctg ctaataacac cacgcactcg aaggataaat
     2941 gggcagtgtt catggcgatt gtgcacagcg accagcgtaa ttcatcaata tcttccatcg
     3001 agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa cgttcgctat
     3061 cagaggcaag taacgcgcga aaggcgagca gaatttcatg ttctgctacc tcatattctt
     3121 ctggttgcca aacagattta gcaggcgact gcgcaatccg gagcgcctcc tcatacgcct
     3181 gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacggt aacagcgcca
     3241 gataagcgcg gcggataata tcgacgtcag tcgtttcctc tatttcgaga atcttccagc
     3301 aatttttcat aggtcgctca tatccttaag cgtttccggg catgcagaat agtaagccaa
     3361 tggttgccag gacaaggtag ggtaatacgc atcatcactg gcaaataatt gataaaacat
     3421 atcattgccg gaatcgtcgt attctttacg cgctaagaat tcgcgtaata actgcagatg
     3481 atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa agtattgccg
     3541 ccagttacga taataacgta gagcccgtag atgaatgcgc gactggatcc atagactttc
     3601 ttcttcgctc aaccagttat taagtacgcc cattcggctt aaaaaaccca tccggacata
     3661 atcccaggct ttaattcctc cctcaccaca gcattcggcg gtgctggcga caaattgcgc
     3721 ataagcctga ccttgctcat ttagttcagc aagatagtca cgccattcac atggcgaata
     3781 acgaaaccag cggtgataaa accctgccaa ccgagcggca tgaccgttat cgatcattcg
     3841 gtgaattgtg cgcagcaggt catcacgggc atcaattccc caggaggaag aaaggtcaaa
     3901 aaagcatgta cctaaataca ttttgggtga tgaatgcgaa ggtaaacagc attcattgcg
     3961 tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gactccatgt ccatataatt
     4021 tatccttaaa tattcattaa atgaatatta gccgcattgc gaggctcgtt tatttatact
     4081 tttctgacgc gacataatac gcataaaata aggcttacgc aaaaatggaa cacataaaaa
     4141 tataatatgt tttatctgca accataagga aatgataatg attttcacgt caagttgctg
     4201 cgataattta tcaatagatg agattatcga acgtgctgaa aaaggcgatt gcgaggctca
     4261 atatattgtt gggttttatt ataatcgcga tagcgcaatt gattctccgg acgacgaaaa
     4321 agccttttac tggctgaagc tggccgctga gcaaggtcat tgtgaagcac agtattcctt
     4381 agggcaaaag tataccgagg ataaaagccg tcataaagat aatgagcaag ccatcttctg
     4441 gctgaaaaaa gctgccctac aaggccatac tttcgcttcc aacgcccttg gctggacact
     4501 ggatcgtgga gaagccccca attataaaga agcggttgtc tggtatcaga tagccgcgga
     4561 gagcggaatg tcttatgcgc aaaataatct tgggtggatg tacagaaatg gcaacggagt
     4621 cgcaaaagac tatgcgctgg catttttctg gtacaaacaa gctgcattac aaggccatag
     4681 tgacgcgcaa aacaatctgg ccgatcttta tgaagacgga aaaggcgttg ctcaaaacaa
     4741 gacactcgcc gcattctggt atttgaaaag cgcacagcag ggtaatcggc acgcccagtt
     4801 tcaaattgca tgggattata acgctggcga aggggtggac caggactata agcaagcgat
     4861 gtactggtat ctgaaggctg ccgctcagga aagcgtcggc gcttacgtca acatcggtta
     4921 tatgtataaa cacggacaag gcgttgagaa agattatcag gctgcctttg aatggtttac
     4981 gaaagccgct gaatgcaatg acgccactgc ctggtataac ctggccatta tgtatcacta
     5041 cggagaagga agacctgtcg atctccgaca ggctctcgac ctgtatcgta aagttcagtc
     5101 atccggaacc agagatgtca gtcaggaaat tcgtgagact gaagatttac tgtagagata
     5161 aaaacaaggg ccggggaatt tcattcggcc catattctct ttattgctgt gcgaaatcag
     5221 actattacgg ctcaaacggg cgtctctgga actggtcatg accacatttc gggcatagcg
     5281 tcagcacttc cggtgtgtag atcgggagat ggaagtgaca tttctcgcag accagatttc
     5341 ccagcccgac cacttctccg ctgtgataaa ccccatgatg attgaggtcc tggaaaactt
     5401 cgcgccattc aagctgcgtt ttatcggtga tatctgccag ctcctgccat aagctttctt
     5461 taatcacccg cataaagacg ctgtcagatt cttctttcag gctctcttca tagctcatgg
     5521 cgaactcttc caggtcacgt ctgacagctc gcgtcagctc atcgacctcg gttcgcgtta
     5581 actcccctgt ttttattacg cgctcgcgcg cctgttccac cagtgcgtcg atatcacgtt
     5641 cgccattgcg caggcgttcg ctcagtgacg cgaccagttc acggtaatat tgagcaacct
     5701 tgttcatcgt ttcatctccc gggtcgttaa ctgactataa taatagacgt tctttgttcc
     5761 ttgctttgct aatacgccca caaactctgt aaattaatgt atgaataatc cgggcatcat
     5821 ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg tttgggctat gctatgcgga
     5881 tctgaaaaac cacctcaacg ctacatttgt agccgtattg aaaacaggac cactggctgc
     5941 catgcaagag caataccgcc cggaagagat agaatccaaa gtacagcttc attgggatga
     6001 gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag aagtattact gcctgtctat
     6061 gcttccctat ccttctggtc gactacacat gggccacgta cgtaactaca ccatcggtga
     6121 cgtgatcgcc cgctaccagc gtatgctggg caaaaacgtc ctgcagccga tcggctggga
     6181 cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac aacaccgctc cggcaccgtg
     6241 gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa atgctgggct ttggttatga
     6301 ctggagccgc gagctggcaa cctgtacgcc ggaatactac cgttgggaac agaaattctt
     6361 caccgagctg tataaaaaag gcctggtata taagaagact tctgcggtca actggtgccc
     6421 gaacgaccag accgtactgg cgaacgaaca agttatcgac ggctgctgct ggcgctgcga
     6481 taccaaagtt gaacgtaaag agatcccgca gtggtttatc aaaatcactg cttacgctga
     6541 cgagctgctc aacgatctgg ataaactgga tcactggcca gacaccgtta aaaccatgca
     6601 gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc ttcaacgtta acgactatga
     6661 caacacgctg accgtttaca ctacccgccc ggacaccttt atgggttgta cctacctggc
     6721 ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa aataatcctg aactggcggc
     6781 ctttattgac gaatgccgta acaccaaagt tgccgaagct gaaatggcga cgatggagaa
     6841 aaaaggcgtc gatactggct ttaaagctgt tcacccatta acgggcgaag aaattcccgt
     6901 ttgggcagca aacttcgtat tgatggagta cggcacgggc gcagttatgg cggttccggg
     6961 tcacgaccag cgcgactacg agtttgcctc taaatacggc ctgaacatca aaccagttat
     7021 cctggctgct gacggctctg agccagatct ttctcagcaa gccctgactg aaaaaggcgt
     7081 gctgttcaac tctggcgaat tcaacggtct tgaccatgaa gcggccttca acgccatcgc
     7141 cgataaactg actgcgatgg gcgttggcga gcgtaaagtg aactaccgcc tgcgcgactg
     7201 gggtgtttct cgtcagcgtt actggggcgc gccgattccg atggtgacgc tggaagacgg
     7261 taccgtaatg ccgaccccgg acgaccagct gccggtgatc ctgccggaag atgtggtgat
     7321 ggacggcatt accagcccga ttaaagcaga tccggagtgg gcaaaaacta ccgttaacgg
     7381 tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt atggagtcct cctggtacta
     7441 tgcgcgctac acttgcccag agtacaaaga aggtatgctg gattccgaag cggctaacta
     7501 ctggctgccg gtggatatct acattggtgg tatcgaacac gccattatgc acctgctcta
     7561 cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg gtgaactctg acgaaccagc
     7621 gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc ttctactatg ttggcgaaaa
     7681 cggcgaacgt aactgggttt ccccggttga tgctatcgtt gaacgtgacg agaaaggccg
     7741 tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt tataccggca tgagcaaaat
     7801 gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg gttgaacgtt acggcgcgga
     7861 caccgttcgt ctgtttatga tgtttgcttc tccggctgat atgactctcg aatggcagga
     7921 atccggtgtg gaaggggcta accgcttcct gaaacgtgtc tggaaactgg tttacgagca
     7981 cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg ctgactgaag atcagaaagc
     8041 gctgcgtcgc gatgtgcata aaacgatcgc taaagtgacc gatgatatcg gccgtcgtca
     8101 gaccttcaac accgcaattg cggcgattat ggagctgatg aacaaactgg cgaaagcacc
     8161 aaccgatggc gagcaggatc gcgctctgat gcaggaagcg ctgctggccg ttgtccgtat
     8221 gcttaacccg ttcaccccgc acatctgctt cacgctgtgg caggaactga aaggcgaagg
     8281 cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa gcgatggtgg aagactccac
     8341 gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa atcaccgttc cggtggacgc
     8401 aacggaagaa caggttcgcg aacgtgctgg ccaggaacat ctggtagcaa aatatcttga
     8461 tggcgttacc gtgcgtaaag tgatttacgt accaggtaaa ctcctcaatc tggtcgttgg
     8521 ctaagcgcgg gaggaagcgt gcgatatctg gcaacattgt tgttatctct ggcggtgtta
     8581 atcaccgccg ggtgtggctg gcatctgcgt gataccacgc aggttccttc cactatgaag
     8641 gtcatgatcc tggactcagg cgatccgaac gggccattaa gccgtgcggt gcgtaaccag
     8701 ttacgtctga atggtgtcga gttgcttgat aaagaaacca cgcgtaagga cgttccatcc
     8761 ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat cggtattccg taacggtcaa
     8821 acagcagagt atcagatgat catgacggtg aatgcgaccg tgttgatccc cggccgtgat
     8881 atctacccga ttagcgccaa agtcttccgt tcgttctttg ataacccgca aatggcgtta
     8941 gcgaaagata acgaacaaga catgatcgta aaagagatgt acgaccgtgc tgccgaacag
     9001 ctgattcgta agctgccaag catccgtgct gcggatattc gttccgacga agaacaaacg
     9061 tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct ccaccacgct gggtaactga
     9121 tgattcggtt gtacccggaa caactccgcg cgcagctcaa tgaagggctg cgcgcggcgt
     9181 atcttttact tggtaacgat cctctgttat tgcaggaaag ccaggacgct gttcgtcagg
     9241 tagctgcggc acaaggattc gaagaacacc acactttttc cattgatccc aacactgact
     9301 ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt tgccagtcga caaacgctat
     9361 tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa tgaacaactt ctcacactca
     9421 ccggacttct gcatgacgac ctgctgttga tcgtccgcgg taataaatta agcaaagcgc
     9481 aagaaaatgc cgcctggttt actgcgcttg cgaatcgcag cgtgcaggtg acctgtcaga
     9541 caccggagca ggctcagctt ccccgctggg ttgctgcgcg cgcaaaacag ctcaacttag
     9601 aactggatga cgctgcaaat caggtgctct gctactgtta tgaaggtaac ctgctggcgc
     9661 tggctcaggc actggagcgt ttatcgctgc tctggccaga cggcaaattg acattaccgc
     9721 gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc ttttcattgg gttgatgctt
     9781 tgttgatggg aaaaagtaag cgcgcgttgc atattcttca gcaactgcgt ctggaaggca
     9841 gcgagccggt tattttgttg cgcacattac aacgtgaact gttgttactg gttaacctga
     9901 aacgccagtc tgcccatacg ccactgcgtg cgttgtttga taagcatcgg gtatggcaga
     9961 accgccgggg catgatgggc gaggcgttaa atcgcttaag tcagccgcag ttacgtcagg
    10021 ccgtgcaact cctgacacga acggaactca ccctcaaaca agattacggt cagtcagtgt
    10081 gggcagagct ggaagggtta tctcttctgt tgtgccataa acccctggcg gacgtattta
    10141 tcgacggttg atatgaaatc tttacaggct ctgtttggcg gcacctttga tccggtgcac
    10201 tatggtcatc taaaacccgt ggaaacgctg gcgaatttga ttggtctgac gcgggtcaca
    10261 atcatcccta ataatgttcc tccgcatcgt ccccagccgg aagcgaacag cgtgcagcgt
    10321 aaacacatgc ttgaactggc gattgccgac aagccattat ttactcttga tgaacgcgag
    10381 ctaaagcgca atgccccctc ttacactgcg caaacactga aagagtggcg gcaggaacaa
    10441 ggaccggacg tgccgctggc gtttattatt ggtcaggatt cactgctgac ctttccgacc
    10501 tggtacgaat acgaaacgat actcgacaat gcacatttga tcgtctgtcg gcgtccaggt
    10561 tacccacttg aaatggcgca accgcaatac cagcaatggc tggaagatca tttgacacat
    10621 aacccggaag atcttcacct tcagcctgcc ggtaaaattt atctggctga aacgccgtgg
    10681 tttaacatct cggcgaccat catccgcgaa cgtttgcaaa acggtgaatc gtgtgaggat
    10741 ttattgccgg aaccggtact gacttacatt aaccaacaag gcttgtatcg ctgatacctg
    10801 tcgttacgga gggcatgatg cgactgtggt taattcgtca tggtgaaacg caagcgaata
    10861 tcgatggtct atacagcggt catgcgccca cccccctgac cgcgcgcggt attgagcaag
    10921 cgcaaaatct gcatacgctg ctacatggtg tttcctttga tctggtttta tgcagtgaac
    10981 tggaacgggc acagcatacc gcgcgactgg ttctcagtga ccgccagctc cccgttcata
    11041 tcatccctga actcaacgaa atgttttttg gcgactggga gatgcgacat catcgcgacc
    11101 tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa tgactggcag catgcaatcc
    11161 ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt ggaacgcttt atcgcaaggc
    11221 ttagtgaatt tcagcactat cagaatattt tagtcgtcag ccatcagggt gtactgagtc
    11281 tgttaatcgc ccgtttaatt ggcatgcctg ccgaggctat gtggcatttt cgcgttgacc
    11341 aggggtgttg gagcgccatt gatatcaacc aaaaattcgc gacgctacgc gtcctcaata
    11401 gccgtgccat cggggtcgag aatgcatgac ttttctgttt ttttacgggt aagccgcaac
    11461 gaccattgac agacccgggc aggctgatat tctccgcagc cagacttttt tcgccagaca
    11521 cgactttgta gaaattgttt tacaaaaatg gcgatgcaat ctgcggcgcg gggtgggatg
    11581 atagcccact ttcgaaagcc gattcggcga caattgtccc gaaatcgcct ctggttcagg
    11641 tatactgaca gaccattttt atctatttga ttcacccagg gggaaaactt gcagggtaaa
    11701 gcactccagg attttgttat cgacaaaatt gatgacctca aaggtcagga catcatcgcc
    11761 ttagacgttc aaggcaaatc cagcatcacc gactgcatga tcatctgtac gggtacgtcc
    11821 agccgtcatg ttatgtccat tgctgaccac gttgtgcagg agtctcgcgc agcgggcctg
    11881 ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga ttgtcgtgga tttgggcgat
    11941 gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt atgaactgga aaaactctgg
    12001 agttaatgcg tgaagctgca acttgtcgcc gtgggaacga aaatgccgga ctgggtacaa
    12061 accggtttta ccgagtacct gcgtcgtttt ccgaaagata tgcccttcga gctgattgaa
    12121 attccggccg gaaaacgcgg caagaatgcg gacatcaagc gcatactcga caaagagggt
    12181 gagcagatgt tggcggccgc aggcaaaaac cgcattgtca ccctcgatat tccaggcaag
    12241 ccctgggata cgccgcagtt agccgctgag ctggaacgct ggaagctgga tggtcgcgac
    12301 gtcagtctac tgattggcgg gcctgaaggg ttgtcgcctg cctgtaaagc ggcggcggag
    12361 cagagctggt cgctgtcggc gcttaccctc ccccatccgc tggttcgcgt gctggtcgca
    12421 gagagtctgt accgggcgtg gagcatcacc accaaccatc cttatcaccg tgagtgataa
    12481 gggagctttg agtagaaaac gcagcggatg aaactacaga actcttttcg cgactatacg
    12541 gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct ttttggggat tttgctgctg
    12601 accggcgtgc ttatcgccaa cctgtataat ctgcaaattg ttcgctttac cgactaccag
    12661 actcgctcta atgaaaaccg cattaagctg gtgcctatcg cgcccagccg cggcattatc
    12721 tacgaccgta atggtatccc tctggccctc aaccgcacta tctaccagat agaaatgatg
    12781 ccagagaaag tcgataacgt gcagcaaacg ctggacgctt tgcgcagcgt ggttgatctg
    12841 accgatgacg atattgctgc attccgaaaa gagcgcgcac gttcacaccg tttcacctct
    12901 attccggtga aaactaacct gaccgaagta caagtagctc gctttgccgt caatcagtac
    12961 cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt actatcctta cggttcggcg
    13021 ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata aagacgtcga acgcctgaat
    13081 aatgacggca aactggccaa ctatgcggca acgcatgata tcggtaagct gggcattgag
    13141 cgttactatg aagacgtgct gcacggtcag accggttatg aagaggttga agttaacaac
    13201 cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc aagccggaca cgatatttac
    13261 ctgacgctgg atctcaaact ccagcaatat attgaaacgc tgctggcggg tagccgcgca
    13321 gctgtggttg tcaccgatcc gcgtacaggt ggggtgctgg cgctggtttc cacgcctagt
    13381 tatgacccaa acctgtttgt tgacggtatc tccagcaaag attattccgc cttgttgaac
    13441 gacccgaata caccgctggt gaaccgcgcc acacaggggg tttatcctcc cgcgtctaca
    13501 gttaaaccct atgtggcggt ttcggcattg agcgccgggg tgatcacgcg caatacgacg
    13561 ctgtttgacc caggctggtg gcaactgcca ggttcggaaa aacgttatcg tgactggaaa
    13621 aaatggggcc acgggcgtct gaatgtcaca agatcgctgg aagaatctgc ggataccttc
    13681 ttctatcagg tggcctacga tatggggatc gatcgcctct ccgaatggat gggtaaattc
    13741 ggttatggtc attacaccgg tatcgacctg gcggaagaac gttccggcaa catgcctacc
    13801 cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc agggtgacac cattccggtt
    13861 ggtatcggtc agggttactg gacagcgacc ccaatccaga tgagtaaggc actgatgatc
    13921 ctgattaatg acggtatcgt gaaggttcct catttgctga tgagcaccgc cgaagacggc
    13981 aaacaggtgc catgggtaca gccgcatgaa ccgcccgtcg gcgatattca ttccggttac
    14041 tgggagctgg cgaaagacgg tatgtacggt gttgctaacc gccctaacgg tacggcgcat
    14101 aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat ccggtaccgc tcaggtcttc
    14161 ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg ccgagcgttt acgtgaccac
    14221 aaactgatga ccgcctttgc gccatacaac aatccgcaag tggctgtcgc catgattctg
    14281 gagaacggtg gtgcgggtcc ggcggttggg acactgatgc gccagatcct cgaccacatt
    14341 atgctgggtg ataacaacac cgatctgcct gcggaaaatc cagcggttgc cgcagcggag
    14401 gaccattaat catgacggat aatccgaata aaaaaacatt ctgggataaa gtccatctcg
    14461 atcccacaat gctgctgatc ttactggcat tgctggttta cagcgccctg gttatctgga
    14521 gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat cggccaaatc gcgatgggtc
    14581 tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt ttatgaaggc tgggccccct
    14641 atctctatat catctgtatt attttgctgg tggcggtaga tgctttcggt gccatctcta
    14701 aaggtgctca acgctggctg gacctcggta ttgttcgttt tcagccgtcg gaaattgcca
    14761 aaatagccgt accactgatg gttgcgcgct ttatcaaccg cgacgtttgc ccgccatcgt
    14821 tgaagaacac tggcatcgcg ctggtgctga tatttatgcc cacgctgctg gtggctgcac
    14881 agcctgacct gggaacatca atcctcgttg cgctttccgg tctgtttgta ctgttcctct
    14941 ctggccttag ctggcgtctg attggcgtcg cagtagtgct ggtggcggcg ttcattccga
    15001 ttctgtggtt cttcctgatg catgattacc agcgccagcg cgtaatgatg ctcctggacc
    15061 cggaatcaga cccactcggc gcgggctatc acattattca gtctaaaatt gctattggct
    15121 ccggcggatt acgcggcaaa ggctggctgc acggcactca gtcacagctt gaatttctcc
    15181 ccgaacgcca taccgacttt atcttcgcgg tactggcgga agagctggga ttagtgggca
    15241 ttttgattct gctcgctctc tacattctgc tgatcatgcg cgggctgtgg atagccgcca
    15301 gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt aatgctgata ttattcgttt
    15361 atgtcttcgt aaatattggt atggtaagcg gtattctgcc ggttgtaggg gttccgctcc
    15421 cactggtcag ttatggagga tcggcgctaa ttgtgctgat ggctgggttc gggattgtaa
    15481 tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt gtaagaggtg cgcaatgcgt
    15541 aagcagtggc tcgggatctg catcgcggca ggaatgctcg cggcatgtac aagcgatgat
    15601 ggtcagcaac agacggtaag tgtaccgcag cctgcggtat gtaacggccc catagttgaa
    15661 attagcgggg cggacccgcg tttcgaacca ctgaacgcga cggcaaatca ggattaccag
    15721 cgcgacggta aaagctacaa aatcgtgcag gatccgtctc gatttagcca agcgggactg
    15781 gcggcaatct atgatgccga accaggcagt aacctgacgg cctctggcga agctttcgat
    15841 ccgacacagc tgacggcggc ccatccaacg cttccgatcc ccagctacgc cagaatcact
    15901 aacctggcta acgggcgaat gatcgtggtg cgcattaatg atcgcggccc ttacggcaac
    15961 gaccgcgtta tttcgctttc tcgcgcggca gctgaccgtc ttaacacgtc aaacaacacc
    16021 aaagttcgta tcgatccgat tattgtcgcc caggatggtt cgctttctgg tcctggtatg
    16081 gcttgtacca cagtcgccaa acagacttac gccctgcctg cacctcccga tttaagcggt
    16141 ggcgcgggaa caagttcagt gtctggcccg cagggtgaca ttcttccggt cagtaattcg
    16201 acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca gtagcggttt cctcggcgca
    16261 ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac cgacgcctgc gccacagccc
    16321 gttgttacag ctccgtcgac aacgcctgca acctcgcctg caatggtgac accgcaagcc
    16381 gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg gggccgttag cgatcaggct
    16441 cgtgcgcaac agtaccaaca gcaactggga cagaagttcg gcgtccccgg tcgcgtaact
    16501 caaaatggcg cggtctggcg gatccagctt ggccccttcg ccagcaaagc cgaagccagt
    16561 accttgcagc aacgtttgca aaccgaagcc caattacagt catttattac caccgcgcag
    16621 tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat gacgttaaca aagtcacgcg
    16681 gaaagtcaga tgcctgccgg tagtgcattt gctatagtag ggcacttttt ttaattccat
    16741 cacggatgtc gtagttcaga ccatgaatac cattttttcc gctcgtatca tgaagcgcct
    16801 ggcgctcacc acggctcttt gcacagcctt tatctctgct gcacatgccg atgacctgaa
    16861 tatcaaaact atgatcccgg gtgtaccgca gatcgatgcg gagtcctaca tcctgattga
    16921 ctataactcc ggcaaagtgc tcgctgaaca gaacgcagat gtccgccgcg atcctgccag
    16981 cctgaccaaa atgatgacca gttacgttat cggccaggca atgaaagccg gtaaatttaa
    17041 agaaactgat ttagtcacta tcggcaacga cgcatgggcc accggtaacc cggtgtttaa
    17101 aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt ccggtttctc agctgatccg
    17161 cggtattaac ctgcaatcgg gtaacgatgc ttgtgtcgcc atggccgatt ttgccgctgg
    17221 tagccaggac gcttttgttg gcttgatgaa cagctacgtt aacgcactgg gcctgaaaaa
    17281 tacccacttc cagacggtac atggtctgga tgctgatggt cagtacagct ccgcgcgaga
    17341 tatggcgctg atcggccagg cattgatccg tgacgtaccg aatgaatact cgatctataa
    17401 agaaaaagaa tttacgttta acggtattcg ccagctgaac cgtaacggcc tgttatggga
    17461 taacagcctg aatgtcgacg gcatcaaaac cggacacact gacaaagcag gttacaacct
    17521 tgttgcttct gcgactgaag gccagatgcg cttgatctct gcggtgatgg gcggacgtac
    17581 ttttaaaggc cgtgaagccg aaagtaaaaa actgctaacc tggggcttcc gtttcttcga
    17641 aaccgttaac ccactgaaag taggtaaaga gttcgcctct gaaccggttt ggtttggtga
    17701 ttctgatcgc gcttcgttag gtgttgataa agacgtgtac ctgaccattc cgcgtggtcg
    17761 catgaaagat ctgaaagcca gctatgtgct gaacagcagt gaattgcatg cgccgctgca
    17821 aaagaatcag gtcgtcggaa ctatcaactt ccagcttgat ggcaaaacga tcgaacaacg
    17881 cccgctggtt gtgttgcaag aaatcccgga aggtaacttc ttcggcaaaa tcattgatta
    17941 cattaaatta atgttccatc actggtttgg ttaaaaattg aacacttgaa agtgtaattt
    18001 ccgtccccat atactaagca tcagtaaaaa aactcccgcc ttctggcggg agttgctatt
    18061 taattacgtt acgccggagc tgacatgaaa accaaactta acgaactgct tgaattccct
    18121 actcctttta cttacaaagt tatggggcag gcgttacctg agctggttga tcaggtggtt
    18181 gaagtggtac agcgccatgc gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa
    18241 ggcaactacc actcggtatc tatcactatc aacgccactc atatcgagca ggttgaaaca
    18301 ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg ttctgtaact cgcttctccg
    18361 ttacccggct cctggtcggg taactcccct ctcctcgctg tgatatactt ttcccccact
    18421 tttactcatt ctccacggag atgccgtttt gtatcaggat aaaattcttg tccgccagct
    18481 cggtcttcag ccttacgagc caatctccca ggctatgcat gaattcaccg atacccgcga
    18541 tgatagtacc cttgatgaaa tctggctggt cgagcactat ccggtattca cccaaggtca
    18601 ggcaggaaaa gcggagcaca ttttaatgcc gggtgatatt ccggtgatcc agagcgatcg
    18661 cggtgggcag gtgacttatc acgggccggg gcaacaggtg atgtatgtgt tgcttaacct
    18721 gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa
    18781 taccctggct gaactgggta tagaagcgca tcctcgggct gacgcgccag gtgtctatgt
    18841 tggggaaaag aaaatttgct cactgggttt acgtattcga cgcggttgtt cattccacgg
    18901 tctggcatta aacgtcaata tggatctttc accattttta cgtattaatc cttgtgggta
    18961 tgccggaatg gaaatggcta aaatatcaca atggaaaccc gaagcgacga ctaataatat
    19021 tgctccacgt ttactggaaa atattttagc gctactaaac aatccggact tcgaatatat
    19081 taccgcttaa ttccatacat caatggccca atttacactg ggtcattact cataatacct
    19141 gctaatactt tacatctcct tcgcgtttgt atattttctt tatcacttgc agaaatgcgg
    19201 tgccacgcct tgctccgtaa ctcaccccat tgattaacct attgaaatag ttgattatgg
    19261 tgagcaaaag cgttccgttc caattagaca taagattatt gtaatttttt aaatattatt
    19321 tttccatgaa taaaattgga gtaagtgcgt ggatagtaat aatcaaattg aaccctgttt
    19381 aagcagaaag tcgtcagaag gtaaaccaca aatatttaca accttacgaa atattgatct
    19441 taaccttctg actatttttg aagctgtata tgtacataaa gggatcgtta atgcagcgaa
    19501 agtgcttaat ctgaccccct cggcaatcag tcagtctatt cagaaactgc gcgttatatt
    19561 ccctgaccca ttgtttattc gcaaaggcca gggtgtcact cctaccgcat ttgcgatgca
    19621 tctacatgag tatatcactc agggccttga gtccattctt ggcgcgctgg atatcgaagg
    19681 aagctatgat aagcaacgaa cgataactat tgcgaccact ccctcggtcg gagcactggt
    19741 ccttcctgtc atctatcggg cgattaaaac tcactatccg cagcttttac tgcgcaaccc
    19801 gcccatcagc gacgcggaaa accaactcag tcagtttcaa accgatctca tcatcgataa
    19861 catgttttgc accaatcgta cggtgcaaca tcatgttctg ttcaccgaca atatggtgtt
    19921 aatttgccgc gagggaaatc cactactctc tttagaagat gacagagaga ctatcgacaa
    19981 cgctgcgcat gtacttctgt taccggaaga gcaaaatttc agcggtctgc ggcagagagt
    20041 tcaagaaatg tttccggacc ggcaaatcaa tttcaccagc tacaacattt tgacaatcgc
    20101 tgcactggtt gccaacagtg acatgttagc gattattccc agccgttttt ataacctgtt
    20161 tagccgctgc tggccgctgg aaaaactgcc ttttccgtcc ttaaatgagg agcaaataga
    20221 tttctctatc cactacaaca aattcagcct gcgtgatccc atcctgcacg gagtaataga
    20281 tgttatccgt aatgcatttt gaaaaagtat tgttacagcc agatcacagc gcagaacgat
    20341 aaaatggcac aatccaacaa caattttaca ttttcgcgac cgctttggct gctttcaggt
    20401 ccgtttcaat gatatactgc cagtcgttaa ttcaaaaata gttgataatt acaacaatct
    20461 attgaattga aacgctttcc ttcgtaattc gcaactggaa cacgcacgct atgagtaaac
    20521 ccattgtgat ggaacgcggt gttaaatacc gcgatgccga taagatggcc cttatcccgg
    20581 ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca
    20641 agcttccagc ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc
    20701 tgcattctgt ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc aaccacggca
    20761 cagcaacgtt tatgatcctc ggcgctattt gtacccgccg ttgtccgttc tgtgatgttg
    20821 cccacggtcg cccggtagct cctgatgcca atgaaccagt gaaactggcg cagaccattg
    20881 ccgatatggc gctgcgttat gtggttatca cctccgttga ccgtgatgac ctgcgcgatg
    20941 gcggtgccca gcactttgcg gattgcatta ctgccattcg ggaaaaaagc ccgcaaatca
    21001 aaattgaaac tctggtgccg gatttccgtg gtcgtatgga tcgtgctctg gatattctga
    21061 ctgcaacgcc accagatgtg ttcaaccata acctggaaaa cgtaccgcgt atttaccgtc
    21121 aggtacggcc tggtgcagat tacaactggt cgctgaagct gctggaacgc tttaaagaag
    21181 cgcatccgga aatcccgacc aagtctggtc tgatggtggg actgggtgaa accaatgaag
    21241 aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta acgctggggc
    21301 aatatttgca gccaagccgc catcacctgc cggttcaacg ttacgttagc ccggatgagt
    21361 tcgacgaaat gaaagccgaa gcgctggcga tgggctttac ccatgctgca tgcggtccgt
    21421 ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg gatggaagtt aagtaatcct
    21481 gtaataatta cttacatctg tctgataaaa aacccgcttt ttgaagcggg ttttttgtat
    21541 cgaacaagat attgagggag cgtcctgctc gccacgtcac tctttatgag agagcttttc
    21601 agcctgaaga tcaacgtctg cgcctttttt cgccgcagca tcgtcatcat tcatcgcctt
    21661 cttgaaccct ttaatggccg ctccaaggtc tccgcccagc gtacgtaact tcttagtccc
    21721 aaacagcaga acgaccagcg ccgcaactac cagcagtttg gtaatactaa tctcacccat
    21781 agataccttc ttgacatata accggcaaca gaacgcgcct taaaaaccag agaatattaa
    21841 cggtcattcg acgcgcgcac acaatagcaa tctgtaacaa ggtgaatcaa gcgccgagta
    21901 gaaaaacatc ataataattg cggcggcgca aagcgacggt tgtttaagac gggcagttgc
    21961 gcgcgcacct gacgcacacg ttcgggcgtc acttccgcca taatgagtgc aggcatttct
    22021 gacgctgccg caatggtgac gccaaaggga tctataatcc ggctttgacc gatatttttg
    22081 ttcccgcact cccccgccgc caccatataa caggtggtat ccagcgcacg agcggcaagc
    22141 aacgttgacc agtgatgctc tttgagcggc ccgcgaaccc aggcggcagg aagtaccagg
    22201 atttcagctc cctgtaatgc ctgtgccagc gccagctctg gaaagcgtaa gtcataacag
    22261 gtcatcagac cgaccttcat cccctccacc tcgagtaacg gagcgatttc attaccagca
    22321 tcaacacggc gtgattcctg aatggcaaat gcatcataga gatgcaattt ggcataacgg
    22381 gcgacgatgt ttcctgcctg tagcgccacc agcatattcc atgcgcgccc cggcgttgaa
    22441 ggaacatgaa tcgtcagaat tgtcgtcatc atgttacgtt tactttctcg ccgtaaacgt
    22501 ccgaggaatt cgccttccag cagctgtgct gatttaaccg atagatctgc atcatgatca
    22561 tcgcgcgcca gcaatgcttc cggcaggacg aacagcgata cgtcgttttc cgccgcctgc
    22621 gccatcaacg aggcacaaat ctcagcgttc ttttcccaca cagatgtaac agcaaactgt
    22681 cctgcggcaa ccagcattgc cacctcctgt cagagtcgta aaattcgcca acgacgatta
    22741 ttcggcgtta cacttatcac tcatacaaat caaatagcag gattttgcag tgttacaact
    22801 tcttttagca gtttttattg gcggtggtac gggaagcgtg gcgagatggc tgttaagtat
    22861 gcgatttaac ccgctgcatc aggcgattcc gttggggacg ctggcagcaa acctgattgg
    22921 ggcattcatc ataggaatgg gattcgcctg gttcagcagg atgacgaaca ttgatccagt
    22981 gtggaaagta ttaatcacca ccggattttg tggcggtcta acaaccttct caacattttc
    23041 ggcagaagtg gtgtttttgt tacaagaggg ccgctttggc tgggcgttac tgaacgtttt
    23101 cgtcaacctt ctggggtctt ttgccatgac agcactggct ttctggctgt tttcggcctc
    23161 aaccgcacac taaagaccaa aaaaaacccg ctgattaagc gggttttgaa ttcttgctga
    23221 cgtatcttac agagcgatta cgtttgcagc agaagggcct ttggcaccgt tagtgatttc
    23281 gaactctacg cgctgacctt cagcaagagt tttaaaacca ttagtctgga ttgcagagaa
    23341 gtgtacgaac acgtctttgc tgccgtcttc cggagtaatg aaaccgaatc ctttggactc
    23401 attaaaccac ttaacgttac ctttaatctt agacatcaaa attaccttta catgaaaaat
    23461 agacacaaat gctgtgtcgg ttaccagtac accaattgtg gtacgctttg tccagtgcaa
    23521 ctttactaaa aagtgataaa agtcgcgtta tttttgatgt tacttatttt agctattgat
    23581 tttaaagaag ttactaaaac ttcatttgtc tcaaaactga aagcgcatcc aggcaaagta
    23641 cacattgcca ttgttgtagg tacccggaat gtaggtcatc tgaaaagtca ctgggccata
    23701 acccacggag gccaatggca gtagaaccgg gagagggatg taattccagt tatcgcgtgc
    23761 cgttacgcca gcggtgaatc ccagacctaa atgaaaattt tcatccgcca gcggtcgcca
    23821 ggtactttcc catccgtatc cggcaatcgg ttcccatttg ttccacgagt ccttaaatgc
    23881 catggcatac aggccatgcc agtttccttt ttcatcccaa cgcgacaggc caaaaccgcc
    23941 accccacggt cgctcgttat agcgatcggt tttttctttg tcgtaagcga aacgtgcatg
    24001 ccaggtgatg gcaggaatat ataaatcata atgttcaggc tgttgccagg tttgtgcaat
    24061 attttctcta aacgttgtca tccactcatc tgcgttagca aaaactttac caacgctgat
    24121 taactgaata aaaacaaagg aaaagatagc gacatattta ctcacgttca tttgtgacca
    24181 taaaacattt atcaaaaatc tactactagc atagcaaagc tacaattaac ataaccttaa
    24241 tagacccaac ataaagaata atctgaatag ctgctctatt tatttccgct tgttcaagtt
    24301 aaatatttcc gcgcatcgat taaagatgat gcgtcgtttt ctgcgatggg aatagtcaaa
    24361 aaagaaaaac caagtctttt ttgatgacaa aatgcaatca aggaaaaatt attttatttt
    24421 ttaactagat acaacaatca tcttaacgaa gtatataata taaatcgtca acccatgctc
    24481 atgttagatt attttttgag cgcacagccg cagctaacac cattgcaatt aacaaatttg
    24541 catcaatcca ccatcaattt acacacatta ttatgtgata attgccaacc gctaaatatg
    24601 cgttttgtta tctatgtata aaaacagcaa cttcaatgtc ttaatggcag tttttcttga
    24661 ttttaatcag cattcatcgc caatttattg ggcatatttt ttccttaagc tttaggaatt
    24721 ttttattatt tactttgggg cctggagaca ggaaaaatta tgctgacatt cattgaactc
    24781 cttattgggg ttgtggttat tgtgggtgta gctcgctaca tcattaaagg gtattccgcc
    24841 actggtgtgt tatttgtcgg tggcctgtta ttgctgatta tcagtgccat tatggggcac
    24901 aaagtgttac cgtccagcca ggcttcaaca ggctacagcg ccacggatat cgttgaatac
    24961 gttaaaatat tactaatgag ccgcggcggc gacctcggca tgatgattat gatgctgtgt
    25021 ggatttgccg cttacatgac ccatatcggc gcgaatgata tggtggtcaa gctggcgtca
    25081 aaaccattgc agtatattaa ctccccttac ctgctgatga ttgccgccta ttttgtcgcc
    25141 tgtctgatgt ctctggccgt ctcttccgca accggtctgg gtgttttgct gatggcaacc
    25201 ctatttccgg tgatggtaaa cgttggtatc agtcgtggcg cagctgctgc catttgtgcc
    25261 tccccggcgg cgattattct cgcaccgact tcaggggatg tggtgctggc ggcgcaagct
    25321 tccgaaatgt cgctgattga cttcgccttc aaaacgacgc tgcctatctc aattgctgca
    25381 attatcggca tggcgatcgc ccacttcttc tggcaacgtt atctggataa aaaagagcac
    25441 atctctcatg aaatgttaga tgtcagtgaa atcaccacca ctgctcctgc gttttatgcc
    25501 attttgccgt tcacgccgat catcggtgta ctgatttttg acggtaaatg gggtccgcaa
    25561 ttacacatca tcactattct ggtgatttgt atgctgattg cctccattct ggagttcctc
    25621 cgcagcttta atacccagaa agttttctct ggtctggaag tggcttatcg cggaatggca
    25681 gatgcgtttg ctaacgtggt gatgctgctg gttgccgctg gggtattcgc tcaggggctt
    25741 agcaccatcg gctttattca aagtctgatt tctatcgcta cctcgtttgg ttcggcgagt
    25801 atcatcctga tgctggtatt ggtgattctg accatgctgg cggcagtcac gaccggttca
    25861 ggcaatgcgc cgttttatgc gtttgttgag atgatcccga aactggcgca ctcttccggc
    25921 attaacccgg cgtatttgac tatcccgatg ctgcaggcgt caaacctggg ccgtaccctt
    25981 tcgcccgttt ctggcgtagt cgttgcggtt gccgggatgg cgaagatctc gccgtttgaa
    26041 gtcgtaaaac gcacctcggt accggtgctt gttggtttgg tgattgttat cgttgctaca
    26101 gagctgatgg taccaggaac ggcagcagcg gtcacaggca agtaaatagt aatgccggag
    26161 gcaagtttcc tccggcttta tcatgtcacc cactgtggta tatgcgttgc ggtctgccaa
    26221 ctttgccgtg aacaatttca gcaataatca gatggcggct ggcgcaatat tcaagataac
    26281 gcctggcagt ggtgcgactg atggtcagtg cctgcgccac cgtttctgcc gtatgttgca
    26341 caccaggctc tttaaacagt tttcgcaccg cgtttagcgt caagggatca atgccggtcg
    26401 gcagttcgtc tttaggttca ccgcgagcat aggcattaaa catctcatca atttgcttct
    26461 ggctggcgct atcaatactt tccagcatat gtttacgctg gcggaaacgg gttagcgttt
    26521 gccccagccg ttcataggca atgggcttaa tgagataatc aaatacacca caacgtacgg
    26581 cttcagacac cgtttccata tcgctggctg cagtggtaaa caccacgtcg ccgggataat
    26641 gcgcctgcac cagttcatgc aataaattaa tccctctgcc gtcaggaaga tagttatcga
    26701 gcaagattag ccccggctta aaacgctcga tcatcattcg ggcctgcgcc aggtttcccg
    26761 ccagtaatat ctgactgaat ccgggaatgt gacgaatata ttccgcatgc atctctgcca
    26821 gcggcgtttc gtcctcaacg atcaataggg ttaatggagc tgtcattagg tttcactttc
    26881 ggaatataga ttgaaaacaa ggtaccgcag ggatcattat cttcgagagt gataacacca
    26941 ccgcagcgcg ttacgtagct ggcaatcaag tacaacccaa tgccatgttc accgggctcg
    27001 tcagcacgcg tactgacccc ctgctcaaat attttgtctc gtagagactc tggaacgccg
    27061 cagccctgat cggcgacttc aatcaccaca tcatcgcctt catcgctgag gaataattca
    27121 acgatcttgt ttccttcatc gctacgcagg ctggcttcga aggcgttatc aagtaaattg
    27181 cccacaatgg ctgcaaactc ggtgctatcc agtcctggcg gcagttgcga aagctggcta
    27241 ccggggacaa tgatcatttt tagccccagt tcccgggcgc gctgcacttt accaaaaagc
    27301 agccccgcca cctggcgatc ggcaaacgcc tcacgcaggc tgtcaataag ctgttgctgg
    27361 gcctgagact ccccctgcac catcgccagc acgcgatcat actctttcat ctgcaacaga
    27421 ccattgagcg tcgacatcca attgagatgc tcgtgtcgca atgtacgaag gctctcgacg
    27481 tattgtttta tttgcgtcag ttgcgcattg agggtggaaa tttcgtcttt actacgaaag
    27541 ctgataatgg cccccagcaa atcatcacct gaacgaatag cttcccgatt ggcaataacg
    27601 ctcagaccgt taaagttagc caccacatcc tgacgttttt catcaatctg ttcggtaaag
    27661 aaatcggcgg gcctgaccac ttcagcaatg ggtttaccca accattgccg tccgggagag
    27721 ctcagcccca gcatctttct tgcgttacga ttgatggcgg taatgtaacc atgcggatcc
    27781 accgcaatca gcccttcata aaccgaacta aacagcgcct cttgctgacg gaccacgcgt
    27841 gcgatttgct ttggctccat gcccatcatt tgccgacgga tatgcgctgc caggaaccac
    27901 gacagcaaca tcagaatccc taacagcacg acaaacacgc ctgccatcgg taataaaaac
    27961 tcagcccgcc agctatcgat tttactcacc aggtagccaa tcgacaccac gccgatgact
    28021 tttccatcgt catcaaagat tggcgttttg gcgcgcatcg ccatccccat tgaccctttc
    28081 ccggtaatga agtagctctc ccctttctcc agcgcgccct gtttggtgaa ctgcatagga
    28141 taaccaattt tctccggatt aggatggtaa aggcggatcg agtgccggtc cccaatcacc
    28201 acataatcaa aatcggtatc tctttgtaat ttgttagcga tggtcgccag ccgtttgtag
    28261 tcacgcgttt tcaccgcaga gatgacactg tcattggagg caataatttt cgcctgattc
    28321 attgccatgt cgcgtacatg aagcgttaaa tagtcctcaa aactggccgt aaaatattgc
    28381 gccagggctg caatgacaaa tattgagaac accagaatca gcaaaaagat acgcagcgga
    28441 aatgccagtc tttggaaaaa tgcaaactgt ttattctcgt taagctgcaa cattgttttc
    28501 cttacgggtt gtcccgagcc tctttgacgg atgtaattaa aatgttgaat caaaatacat
    28561 agatattaat tatgatcgtg gcaaatttta ttcattaaat caattaaatc acttaaacct
    28621 gcaggtcatt tcgtaattac attaattaaa aaacttaaaa ccattaaata aataaaaaac
    28681 cactaactct atgtgaaata aatcaaaatt tcacgcagaa atactcctta ggatgtatag
    28741 cgaaaggaga aaaagatata cctcgatcac ccccctttct cccaagtgaa aataaaaggt
    28801 tatcagtttg caacattgaa caacattcgt tgcaaatcga taacaacatg caccttcagg
    28861 atactattta ttatgttcgg caatgatatt ttcacccgcg taaaacgttc agaaaataaa
    28921 aaaatggcgg aaatcgccca attcctgcat gaaaatgatt tgagcgttga caccacagtc
    28981 gaagtattta ttaccgtaac ccgcgatgaa aagcttatcg cgtgcggtgg aattgccgga
    29041 aatattatta aatgcgttgc gatcagtgaa tccgttcgcg gtgaaggact ggcgctgaca
    29101 ttagccactg aactgataaa cctcgcctat gagcggcaca gcacgcatct gtttatttat
    29161 accaaaaccg aatacgaggc gctgttccgc cagtgcggtt tttccacgct gaccagcgta
    29221 cccggcgtga tggtgctgat ggaaaacagc gccacgcgac tgaaacgcta tgccgaatcg
    29281 ctgaaaaaat ttcgtcatcc agggaacaag attggctgca ttgtgatgaa cgccaatccc
    29341 ttcacgaatg gtcaccgtta tctgattcaa caagctgcgg cacagtgcga ctggttgcat
    29401 ctgtttttag tcaaagaaga ttcttcacgc ttcccctatg aagaccggtt ggatctggtg
    29461 ttaaaaggca ccgccgatat tccacgcctg actgtgcatc gcggctccga atacatcatc
    29521 tcccgcgcta cgttcccttg ctacttcatt aaagaacaga gcgtcattaa ccattgttac
    29581 accgaaattg atctgaagat tttccgtcag tacctcgctc ccgcgctggg tgtaactcac
    29641 cgctttgtcg gtactgaacc cttttgtcgc gttaccgccc agtacaacca ggatatgcgc
    29701 tactggctgg aaacgccgac tatctccgca ccgcccatcg aactggttga aattgagcgg
    29761 ctgcgttacc aggagatgcc gatatccgct tcccgggtac gtcaactgct ggcgaaaaac
    29821 gatctcacgg ctatcgcgcc gctggtccct gcagtcacgc tgcattattt gcagaacctg
    29881 cttgagcact cccgccagga cgcggcagct cgtcaaaaga cccccgcatg agaaacaggt
    29941 gaaaaatgaa aataaaccag cccgccgttg caggcaccct tgagtctggg gatgtgatga
    30001 tacgcatcgc cccactcgat acgcaggata tcgacctgca aatcaatagc agcgttgaga
    30061 aacagtttgg cgatgcaatt cgcaccacca ttctggacgt tctcgcccgc tacaacgtgc
    30121 gcggcgtaca gctgaatgtc gatgacaaag gcgcactgga ctgcatttta cgtgcacgac
    30181 tggaagccct gctggcacgc gccagcggta tcccggctct gccatgggag gattgccaat
    30241 gatttccgct tcgctgcaac aacgtaaaac tcgcacccgc cgcagcatgt tgtttgtgcc
    30301 tggtgccaat gccgcgatgg tcagcaactc cttcatctac ccggctgatg ccctgatgtt
    30361 tgacctcgaa gactccgtgg cattacgtga aaaagacacc gcccgccgca tggtttatca
    30421 cgcgctgcaa catccgctgt atcgcgatat tgaaaccatt gtgcgtgtca acgcgctgga
    30481 ttccgaatgg ggtgttaacg acctggaagc cgtcgttcgc ggtggtgcgg acgttgtgcg
    30541 tctgccgaaa accgataccg ctcaggatgt tctggatatt gaaaaagaga tcctgcgtat
    30601 cgaaaaagcc tgtggtcgtg aacccggcag caccggcctg ctggcggcga ttgaatctcc
    30661 gctgggcatt acccgcgcag tggaaatcgc tcacgcttcc gagcgtttga tcggtatcgc
    30721 cctcggtgca gaagactatg tgcgcaacct gcgtacagaa cgctccccgg aaggaactga
    30781 actgctgttc gcacgctgtt ccattttgca ggccgcgcgc tctgcgggta ttcaggcgtt
    30841 cgataccgtc tattccgacg ctaacaacga agccggattc ctgcaagaag ccgcccacat
    30901 caaacagctg ggctttgacg gcaaatcgct gatcaacccg cgtcagattg atctgttgca
    30961 caacctctac gcaccaaccc agaaagaagt ggatcacgcc cgccgcgtcg tagaagccgc
    31021 tgaagccgcc gctcgcgaag gcctcggcgt ggtttccctg aacggcaaga tggtggacgg
    31081 tccggttatc gatcgcgccc gtctggtgct ctctcgtgca gaactttccg gcatccgcga
    31141 agaataaggc aatcaaaatg acgcagaaaa ttgaacaatc tcaacgacaa gaacgggtag
    31201 cggcctggaa tcgtcgcgct gaatgcgatc ttgccgcttt ccagaactcg ccaaagcaaa
    31261 cctaccaggc tgaaaaagcg cgcgatcgca aactgtgcgc caacctggaa gaagcgattc
    31321 gtcgctctgg tttacaggac ggcatgacgg tttccttcca tcacgctttc cgtggcggtg
    31381 acctgaccgt caatatggtg atggacgtca tcgcgaagat gggctttaaa aacctgaccc
    31441 tggcgtccag ctccctgagt gattgccatg cgccgttggt agaacacatt cgccagggcg
    31501 tggttacccg catttatacc tccggcctgc gcggtccact ggcggaagag atctcccgtg
    31561 gtctgctggc agaaccggtg caaatccact ctcacggcgg tcgcgtgcat ctggtacaga
    31621 gcggcgaact gaatatcgac gtggctttcc tcggcgtccc gtcctgcgat gaattcggta
    31681 atgccaacgg ctacaccggt aaagcctgct gcggctccct cggctatgca atggttgatg
    31741 ccgacaacgc aaagcaggtc gtgatgctta ccgaagaact gctgccttat ccgcataatc
    31801 cggcaagcat tgagcaagat caggttgatt tgatcgtcaa agttgaccgc gttggcgatg
    31861 ctgcaaaaat cggcgctggc gcgacccgta tgaccactaa cccgcgcgaa ctgcttattg
    31921 cccgtagcgc tgcggatgtg attgtcaact caggctactt caaagaaggt ttctccatgc
    31981 aaaccggcac cggcggcgca tcgctggcgg taacccgttt cctggaagac aaaatgcgta
    32041 gccgcgatat tcgcgccgac ttcgccctcg gtggtattac cgcgacgatg gttgatctgc
    32101 acgaaaaagg tctgatccgc aaactgctgg atgtacagag ctttgacagc catgctgcgc
    32161 aatcgctggc ccgtaacccc aatcacatcg aaatcagcgc caaccagtac gctaactggg
    32221 gttcgaaagg cgcatcggtt gatcgtctcg acgtggtggt actgagcgcg ctggaaattg
    32281 acacccagtt caacgttaac gtgctgaccg gctctgacgg cgtactgcgt ggtgcttccg
    32341 gtggtcactg cgataccgcg attgcctctg cgctttccat catcgtcgcg ccgctggtac
    32401 gcggtcgtat tccgactctg gtggataacg tgctgacctg catcaccccg ggctccagtg
    32461 tcgatattct ggtcacagac cacggtatcg cagttaaccc ggcacgtccg gaactggcag
    32521 aacgtctgca ggaagcgggc attaaagtgg tttccattga gtggctgcgc gaacgtgcgc
    32581 gtctgctgac cggtgaacca cagccgattg aattcaccga ccgcgtcgtt gccgttgtgc
    32641 gttaccgcga tggctcggtg atcgatgttg tgcatcaggt gaaggaataa gccatgcacc
    32701 tgcttcctga actcgccagc caccatgcgg tgtcaattcc cgagctgctc gtcagccggg
    32761 atgaaaggca agcacggcaa cacgtctggc tcaagcgcca tcctgttcca ctggtctcct
    32821 ttaccgtggt tgcgcctggg ccgattaaag acagtgaggt cacacgccga atttttaatc
    32881 atggcgtgac agccttgcgt gccttagccg caaaacaggg ctggcaaatt caggagcagg
    32941 ctgcactggt ttccgccagc gggccggagg gtatgttgag cattgccgcc ccggctcgcg
    33001 acctcaagct cgccaccatt gagcttgaac atagtcatcc tctcgggcgg ttatgggata
    33061 tcgatgtcct gacgcccgaa ggcgaaattc tctcccgccg cgactattca ctgccgcctc
    33121 gcagctgcct gttgtgcgag caaagcgcag ccgtctgcgc gcgtggaaaa acccatcaac
    33181 tgaccgattt actcaaccgc atggaggcac tgctgaacga tgtcgatgcc tgcaacgtca
    33241 actaaaacca caaagcttgc gacgtcatta atcgatgagt acgccctgct gggctggcgc
    33301 gccatgctga ctgaagtcaa tctgtcaccg aaaccaggcc tcgtggatcg gattaactgc
    33361 ggtgcgcata aagatatggc gctggaagat ttccaccgca gcgcgctggc gattcagggc
    33421 tggctacccc gtttcattga atttggtgcc tgtagtgcgg aaatggcacc agaagcggta
    33481 ctccacggat tacgcccaat tggtatggct tgcgaaggtg atatgttccg cgccactgcg
    33541 ggcgtaaaca cgcataaagg cagcattttt tctttagggc tgctatgtgc ggcaattggc
    33601 cgtttgcttc agctcaacca gccggtaacg ccaacaaccg tttgttctac ggcggcaagt
    33661 ttctgccgtg gtctgaccga tcgcgaactg cgtaccaata attcacaact gacggcaggt
    33721 caacggttgt accagcagct tggccttacc ggcgcacgcg gtgaagccga agcgggttat
    33781 ccactggtga tcaaccacgc cttgccgcat tacctcactc tgctggatca ggggttagat
    33841 cctgaactgg cattgctcga taccttgctc ctactgatgg cgatcaacgg cgataccaac
    33901 gttgcttcgc gcggtggcga ggggggcctg cgctggctac agcgcgaggc gcaaacatta
    33961 ttgcaaaaag ggggcattcg aacccccgcc gatctcgatt atctccggca gttcgacagg
    34021 gagtgtatcg aacgaaatct cagtccaggc ggcagtgccg acctgctgat ccttacctgg
    34081 tttttagcac agatttaatt atttaagcac ttgataaatt tgaaaatatt aattttcgga
    34141 gaacccgtat gtctttagca aaagataata tatggaaact attggcccca ctggtggtga
    34201 tgggtgtcat gtttcttatc cctgtccccg acggtatgcc accacaggca tggcattact
    34261 tcgctgtgtt tgtggcaatg attgtcggca tgatcctcga gccaattccg gcaacagcga
    34321 tcagttttat tgcggttact atttgcgtta ttggcagtaa ttacctgctc tttgatgcca
    34381 aagaattagc tgacccagcg tttaatgcgc aaaaacaggc gctgaaatgg ggtctggctg
    34441 gtttttccag caccacggta tggctggtat ttggcgcatt tatttttgca ttagggtatg
    34501 aagtttccgg gttaggtcgt cgcattgccc ttttcctggt gaaattcatg ggcaaacgca
    34561 cgctgacgtt gggttatgcg attgtcatta tcgacattct gctggcaccg tttacaccgt
    34621 ccaacaccgc gcgtaccggg ggtacggttt ttccggtcat taaaaacctg ccgccgttgt
    34681 ttaaatcatt ccccaacgat ccgtccgcgc gtcgtattgg cggctatttg atgtggatga
    34741 tggtcattag taccagtctg agttcgtcca tgtttgtcac cggtgcggca ccaaacgtgc
    34801 tgggtctgga gttcgtcagc aaaattgccg gtatccagat tagctggttg cagtggttcc
    34861 tctgcttcct gccggttggg gttatcttgc ttatcattgc gccgtggctt tcctacgtgc
    34921 tgtacaaacc ggaaatcaca cacagtgaag aagtggcaac ctgggcgggt gatgaactga
    34981 aaaccatggg tgcgctgaca cgcagagagt ggacactgat tggccttgta ttgctcagct
    35041 taggtttgtg ggtttttggc agtgaagtca ttaatgctac tgcagttggt ctgctggcag
    35101 tttcgctaat gctggccctg cacgttgtgc cttggaaaga tattacccgc tataacagcg
    35161 catggaacac gctggtcaac ctggcaactc tggttgtgat ggctaacggc ctgactcgtt
    35221 ctggctttat tgactggttc gccggtacca tgagtacgca cctggaagga ttctcaccaa
    35281 acgcaacggt gattgtactg gttctggtgt tctactttgc acactacctg tttgccagcc
    35341 tgtctgcgca caccgcaacc atgctgccgg ttattctggc cgtcggtaaa ggtattccgg
    35401 gcgtaccaat ggaacaactg tgtatcctgc tggtgctgtc tatcggtatc atgggctgtc
    35461 tgacgccgta tgcaaccggt cctggggtga ttatttacgg ctgtggctat gtgaaatcaa
    35521 aagattactg gcgtcttggc gcaatcttcg gggtgattta catctctatg ttgctgttgg
    35581 ttggctggcc gattctcgcc atgtggaact aatacgttcg ccaaacgctg aataaatcat
    35641 cacgcccgcc aggttagccc acctggcggg cttttttatg atttaataga tagtattcag
    35701 tgtatgtatg agttccacac ccattatgaa agcattctgg cgtaacgccg cgttgctcgc
    35761 ggtttctctg cttcccttct cttctgccaa cgccgtagcg ttgcaggcaa aacagtatgg
    35821 cgattttgat cgctatgtcc tggccctctc ctggcaaacc ggattttgcc agagtcaaca
    35881 cgatcgaaat cgtaacgaac gagatgaatg tcgcctgcaa accgaaacga ccaacaaagc
    35941 tgattttctg accgtacatg gtctgtggcc aggattgcct aaatcggttg ctgcccgtgg
    36001 tgttgatgaa cgccgctgga tgcgcttcgg ttgcgctact cgcccaatcc cgaatctacc
    36061 agaagcacgc gccagccgaa tgtgttcatc gccggaaacc gggttatcac tggaaacggc
    36121 cgctaaacta agtgaagtca tgccaggagc tggcggacgt tcctgcctgg aacgctacga
    36181 atatgccaaa cacggtgcct gctttggttt tgatccggac gcatacttcg gtacgatggt
    36241 acgcctgaat caagaaatta aagagagcga agccggaaaa ttccttgcgg ataattacgg
    36301 taaaacagtg agccgccgtg actttgacgc cgcctttgcc aaaagctggg gaaaagagaa
    36361 cgtgaaagca gttaagctaa cgtgccaggg taaccctgcg tatttgactg aaattcagat
    36421 ctcgatcaaa gctgacgcca tcaacgctcc gctttctgca aactcattct tgccacaacc
    36481 acacccaggc aactgtggca aaacctttgt gattgataaa gtgggttatt aatcatctga
    36541 tttacaaaac acgctaattc gtcttccata aaccgtgacg attaagaatt atacataatg
    36601 cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg agcaacagtc
    36661 agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct tttgggtatg
    36721 tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga ccaactatca
    36781 tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc gcctatgctg
    36841 gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg tgttcgccag
    36901 aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc aatcttagcg
    36961 atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat agcaatactc
    37021 agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt ggcgattcca
    37081 ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa ctcgaatacc
    37141 agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga taactcgctt
    37201 tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc ctggttctca
    37261 ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca tggcagaagc
    37321 attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg ctgtccttta
    37381 cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc accagacctg
    37441 aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc accttacggc
    37501 actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca aacggcatat
    37561 agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt aataattctc
    37621 ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta tcaaaggcgt
    37681 cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta acaatgccgc
    37741 cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc tccagtttca
    37801 ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg gcgtctatta
    37861 ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc ggatcgctat
    37921 cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc aagcggtagg
    37981 gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag gcgattgtca
    38041 acaatcccac tggaccggca ccatagactg caacgcttga accttgttgg atctgcgcat
    38101 tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct ttatcatctg
    38161 aaagcaaagg cggtacttta aacggcccca cattcccttt agggacgcgg acatattccg
    38221 cctgtccacc aggaacgcca ccatacaggt gactataacc aaacaatgcc gcgggagctg
    38281 gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg caggcggcat
    38341 attgctgcat tcgacagaaa aaacagtcgc cacaagcaat gacaaacgga attaccaccc
    38401 ggtcgccttt ttgcaaattt tttacgtcct ttccggtttc aacgacttcc cccataaatt
    38461 catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa agatggaggt
    38521 cagagccaca gatcgccgtt gccgtaatac gcagaataat atcatctgcc tgttcaatgc
    38581 ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc aatgctttca
    38641 tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta tcacagcggg
    38701 aaaacgggcg gtaccgcagg ttttgggaga attcctggtg ttcagtgaga atatattaat
    38761 cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa tattataatc
    38821 atggcgaatg atttgttcat gattaacagg gagaaagggt tatgtataag acaatcatta
    38881 tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac gctgaattcc
    38941 tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca gccagcctga
    39001 gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa catgaagcag
    39061 aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc attaaacaac
    39121 atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa ctgggggctg
    39181 atgttgtagt tattggttct cgcaacccat cgatttcgac ccatctgtta ggttctaacg
    39241 cctcgagcgt gatccgccat gccaatctgc cggtgctggt tgtgcgttaa ttgccagaat
    39301 aaatatcccg ccctgcccgg agccatcccg gcagggcttt ttaatgccat aaaaaagccg
    39361 ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc ttatgcagtt
    39421 ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc gccagtggcg
    39481 atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaatac gcctttgacg
    39541 ttggtttcgc atttcgcatc aatgataatc tcacccatgc ggttacgttc gactgcgcct
    39601 tccagccagt tggtgttcgg cagcagacca atctggacga aaataccggc cagttcgatg
    39661 ttgtgaatat cgccgctgac acgatctcga tattccagac caacgacttt gctgccgtcg
    39721 cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag gctgcgcagt
    39781 ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attcgagcag cgttacgtgc
    39841 tcaacgatac ccgccaggtc aattgccgct tccacgccgg agttaccgcc gccgataacc
    39901 gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt cacgcctttg
    39961 gtgcgatact gatcttcgcc aggaacgttc atgttgcgcc attttgcacc ggtcgccacg
    40021 ataatgctgc gtgctttcag taccgcgcca gaagctgttt taatctgatg cagaccacct
    40081 tcaactgctg ctgggatcag tttgctggcg ctctggctgt cgatcacatc aacgtcgtat
    40141 tcatcaacgt gaactttcag tgcgcctgcc agcttctgcc cttcagtctt cggtacagaa
    40201 atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc gcccatcaga
    40261 ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc cgggccggaa
    40321 ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc acgtttttcc
    40381 gcgcccgtat caattttggc aacgatttca gtcaacgtca tgcggccctg accaaactct
    40441 ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat ttcgttctgg
    40501 aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcagtacgct catcaggttc
    40561 agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt ttcgaattca
    40621 aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt cgacggatga
    40681 ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag cggggagcct
    40741 gcaaaacgtg gcccctggtt ggaacctggg ttggtgatca ggaaagacgg cttacgcacc
    40801 ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat ttcagccaac
    40861 agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa ctcaacaggc
    40921 ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc gagcatgttt
    40981 atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg gatgcagctt
    41041 gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgattaatta cgcgattgcg
    41101 gaacttagat tttaccaacc aggtccagag acggagccag agttgcttca ccttctttcc
    41161 atttagccgg gcaaacttca cctgggtgag aagctacgta ctgtgctgct ttgattttac
    41221 gcagcaggtc agacgcgtca cggccaatgc cttcagcggt aacttcgatt gcctggatga
    41281 taccctgcgg gtcaacaacg aaggtcgcac ggtcagccag accttcatct tcacgcatgt
    41341 tgtcgaagtt acgggtcagg gcgccagtcg ggtcgccgat catcgcatat ttgattttag
    41401 cgatggtttc agagctgctg tgccatgctt tgtgggtgaa gtgagtatcg gtagatactg
    41461 cgtatacgtc tacgcccagt ttctgcagtt cttcgtagtg gtcagcaacg tcacccagtt
    41521 cggtcgggca tacgaaagta aagtcagccg ggtagaagaa gaagacgctc cagcggcctt
    41581 cggtatcttt ttcggtgatt tcgatgaatt cgccgttttt gaatgcctgg tttttaaaag
    41641 gtttaatttt ggtgttaatc aaggacatct atacttcctc cgtgttttcg atgagatgta
    41701 aggtaaccaa tttttgccga ttcggctaat gcgtttccat tatcaaatcg ataagtttta
    41761 ccttacaacc ttcgtaagac aactttgtga actttgcacc gccagcggcg ataaaaagta
    41821 aaatggcccc ctttccaggt gaccgtgtaa tctgaactac cctttggtat cttcagtgcc
    41881 tgcctgcagg ctgaggtgtt gcccttacaa atgcaacaac gacatggatt acaacaccct
    41941 cataaacaaa gggcaatcac ctgatctaag ctcttaccta tgacagtgat aggttatgcc
    42001 ttttactcga cttttgcact gactgaaaag gacaaattaa tgttaaaaaa gatactttta
    42061 ctggctctgc ttcctgcaat cgccttcgca gaggaacttc ctgctccagt aaaagcgatt
    42121 gaaaaacagg gcattacaat catcaaaaca ttcgatgccc ccggaggaat gaaaggttat
    42181 ctcggaaagt atcaggatat gggcgtcacc atctacctga ctccagatgg taagcacgct
    42241 atctctggtt acatgtacaa cgagaaaggt gaaaacctga gtaacacact tatcgaaaaa
    42301 gaaatttacg caccagccgg acgcgaaatg tggcaacgga tggaacaatc ccactggctc
    42361 ctcgacggta aaaaagatgc gccggtcatt gtctacgtct tcgccgatcc gttctgccca
    42421 tattgtaaac agttctggca gcaggcgcgc ccgtgggtag attctggcaa agtgcaatta
    42481 agaacattgt tggttggggt tatcaagcca gaaagcccgg cgacagcagc ggcaattctt
    42541 gcctccaaag atcccgcaaa aacctggcaa caatatgaag cctctggtgg caagcttaag
    42601 ctaaacgtgc ctgcaaacgt aagtacagag caaatgaaag tgttaagtga caatgagaaa
    42661 ctgatggacg atctgggggc aaatgtcacg ccggctatct attacatgag taaggaaaat
    42721 acgctacaac aggccgtggg gttgcccgat cagaaaacgc ttaatatcat tatggggaat
    42781 aaataaaaga taattcatgg atggttgaaa tacatctctg cttttcaacc atcctaaaaa
    42841 agcatgaatt gctcgctctg ccctaagtgg ctatgctatt ttagaaaaac ttaatcctga
    42901 gttaagcaaa aaaatataat caataaaaat atatacgcaa aaaacattga ttaagtgaat
    42961 ataccatgga agaaaaatat aaccggagta gtgtatggcc aatctctacg acttgaaaaa
    43021 gttcgactta aaccttcttg tcatatttga gtgtatttac cagcatttaa gcattagcaa
    43081 agctgctgaa tcactatata tcaccccatc agccgtgagc cagtcgttac aacgcttacg
    43141 tgcgcaattt aatgaccctt tattcattcg ctcaggaaaa ggtatcgcgc cgacgacaac
    43201 agggttgaat ctgcatcacc atcttgagaa aaacctcaaa ggccttgaac aaacaattaa
    43261 tatagtcaat aagtccgaac taaagaaaaa ctttattatc tacggccctc agctcatttc
    43321 ttgctctaat aatagtatgc tgatccgctg cctacggcaa gatgcctctg tcgagattga
    43381 gtgccatgat atcctcatgt cagcagagaa tgctgaagaa ctgttggttc accgcaaagc
    43441 cgatctggtg atcacccaaa tgccagtgat cagccgttcc gtcatttgca tgccattgca
    43501 tacaattcgc aatactctaa tttgtagtaa taagcatccg cgaattactg acaatagcac
    43561 ttatgaacaa attatggcag aagagttcac ccaattaata tccaaatcag caggagttga
    43621 tgatattcaa atggaaatag acgagagatt tatgaatcgc aaaatctcat ttcgtggttc
    43681 ctctttgctg acaatcatta atagcattgc agttacagat ttactgggaa tagtccccta
    43741 tgaactttat aattcctacc gtgactttct taatttaaaa gagataaaac tagaacatcc
    43801 gttaccttca ataaagcttt acatttccta taataaatct tctttaaata atttggtgtt
    43861 ctcccgattt attgaccgtc taaatgagag tttttgatat ataactacag acagctacta
    43921 taactttcat ctatttattc acagcgcgaa tgaaaaataa aacactctgc tttacagtgt
    43981 ttactttata attaacagtc ttatctcact gttcaccctg taacgttcag agagcgtctt
    44041 cagatgtcta tttataaaat tcctcttccg ctcaatatt
//
LOCUS       DAEVZA010000025        43593 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEVZA010000025 DAEVZA010000000
VERSION     DAEVZA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.025"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            373..1005
                     /locus_tag="LAF92_001423"
     CDS             373..1005
                     /locus_tag="LAF92_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9790460.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1339..2148
                     /locus_tag="LAF92_001424"
     CDS             1339..2148
                     /locus_tag="LAF92_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI9790461.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            2141..2623
                     /locus_tag="LAF92_001425"
     CDS             2141..2623
                     /locus_tag="LAF92_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790462.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(2656..2736)
                     /locus_tag="LAF92_001426"
     CDS             complement(2656..2736)
                     /locus_tag="LAF92_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790463.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(2772..3197)
                     /locus_tag="LAF92_001427"
     CDS             complement(2772..3197)
                     /locus_tag="LAF92_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790464.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAENVLTQTT
                     SWTLKSRYVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            3391..3881
                     /locus_tag="LAF92_001428"
                     /pseudo
     CDS             3391..3881
                     /locus_tag="LAF92_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4106..4597
                     /locus_tag="LAF92_001429"
     CDS             4106..4597
                     /locus_tag="LAF92_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBI9790465.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            4932..6308
                     /gene="ygeH"
                     /locus_tag="LAF92_001430"
     CDS             4932..6308
                     /gene="ygeH"
                     /locus_tag="LAF92_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HBI9790466.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
     gene            6476..6694
                     /locus_tag="LAF92_001431"
     CDS             6476..6694
                     /locus_tag="LAF92_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790467.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            6777..6998
                     /locus_tag="LAF92_001432"
     CDS             6777..6998
                     /locus_tag="LAF92_001432"
                     /inference="COORDINATES: protein motif:HMM:NF013618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBI9790468.1"
                     /translation="MRSPKVKFLTIFTFCIFITKMSFASNSCSNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNIKSLDY"
     gene            7002..7253
                     /locus_tag="LAF92_001433"
     CDS             7002..7253
                     /locus_tag="LAF92_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBI9790469.1"
                     /translation="MQINQMHIPLLKKRGIIKDERDLLDNPCLNIKIGTEILYNHFSR
                     CGVTWQCLGTYNAGFAMDNQKKRQQYAPKYILYIPGLMN"
     gene            complement(7298..7930)
                     /locus_tag="LAF92_001434"
                     /pseudo
     CDS             complement(7298..7930)
                     /locus_tag="LAF92_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(8151..8582)
                     /locus_tag="LAF92_001435"
     CDS             complement(8151..8582)
                     /locus_tag="LAF92_001435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790470.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWRKKLPAISGPVNLFIPTR
                     FKDKYLEVESYFVDKSIWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVNEICEQARHYLVEKMFETHSLDMNNSVLASPEDL"
     gene            complement(8585..8806)
                     /locus_tag="LAF92_001436"
                     /pseudo
     CDS             complement(8585..8806)
                     /locus_tag="LAF92_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304051.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8799..9380)
                     /locus_tag="LAF92_001437"
     CDS             complement(8799..9380)
                     /locus_tag="LAF92_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBI9790471.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            complement(9396..10130)
                     /locus_tag="LAF92_001438"
                     /pseudo
     CDS             complement(9396..10130)
                     /locus_tag="LAF92_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
     gene            complement(10171..10458)
                     /gene="sctI"
                     /locus_tag="LAF92_001439"
     CDS             complement(10171..10458)
                     /gene="sctI"
                     /locus_tag="LAF92_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HBI9790472.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKEGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            complement(10478..10718)
                     /locus_tag="LAF92_001440"
                     /pseudo
     CDS             complement(10478..10718)
                     /locus_tag="LAF92_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(10732..11914)
                     /locus_tag="LAF92_001441"
                     /pseudo
     CDS             complement(10732..11914)
                     /locus_tag="LAF92_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
     gene            12172..12603
                     /locus_tag="LAF92_001442"
     CDS             12172..12603
                     /locus_tag="LAF92_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HBI9790473.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYHKIRALNKLNIKGTV"
     gene            12674..12967
                     /locus_tag="LAF92_001443"
     CDS             12674..12967
                     /locus_tag="LAF92_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBI9790474.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(13028..13420)
                     /locus_tag="LAF92_001444"
     CDS             complement(13028..13420)
                     /locus_tag="LAF92_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HBI9790475.1"
                     /translation="MKSDVSNSRLMISNPTHIAIGIYFKPHLSPIPLISVRVTNEVAL
                     GVRKYAKEIGIPIITDKKLARKNYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPV
                     QDEQLSSEDKFIEGEDTKIENKDDNLKN"
     gene            complement(13417..14148)
                     /locus_tag="LAF92_001445"
     CDS             complement(13417..14148)
                     /locus_tag="LAF92_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HBI9790476.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERIRKFFLSN"
     gene            complement(14157..14924)
                     /gene="sctT"
                     /locus_tag="LAF92_001446"
     CDS             complement(14157..14924)
                     /gene="sctT"
                     /locus_tag="LAF92_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HBI9790477.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGGFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(14926..15186)
                     /locus_tag="LAF92_001447"
     CDS             complement(14926..15186)
                     /locus_tag="LAF92_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HBI9790478.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(15196..15861)
                     /locus_tag="LAF92_001448"
     CDS             complement(15196..15861)
                     /locus_tag="LAF92_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HBI9790479.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(15851..16837)
                     /locus_tag="LAF92_001449"
                     /pseudo
     CDS             complement(15851..16837)
                     /locus_tag="LAF92_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(16797..16916)
                     /locus_tag="LAF92_001450"
                     /pseudo
     CDS             complement(16797..16916)
                     /locus_tag="LAF92_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(16929..18539)
                     /locus_tag="LAF92_001451"
     CDS             complement(16929..18539)
                     /locus_tag="LAF92_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790480.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            complement(18773..18846)
                     /locus_tag="LAF92_001452"
     tRNA            complement(18773..18846)
                     /locus_tag="LAF92_001452"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18812..18814),aa:Gly,seq:ccc)
     gene            complement(18925..19680)
                     /locus_tag="LAF92_001453"
     CDS             complement(18925..19680)
                     /locus_tag="LAF92_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBI9790481.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            20095..22392
                     /gene="xdhA"
                     /locus_tag="LAF92_001454"
     CDS             20095..22392
                     /gene="xdhA"
                     /locus_tag="LAF92_001454"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBI9790482.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            22403..23281
                     /gene="xdhB"
                     /locus_tag="LAF92_001455"
     CDS             22403..23281
                     /gene="xdhB"
                     /locus_tag="LAF92_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBI9790483.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            23278..23757
                     /gene="xdhC"
                     /locus_tag="LAF92_001456"
     CDS             23278..23757
                     /gene="xdhC"
                     /locus_tag="LAF92_001456"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBI9790484.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVQDCEKTK"
     gene            complement(23797..25575)
                     /locus_tag="LAF92_001457"
     CDS             complement(23797..25575)
                     /locus_tag="LAF92_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HBI9790485.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            26054..27241
                     /gene="ygeW"
                     /locus_tag="LAF92_001458"
     CDS             26054..27241
                     /gene="ygeW"
                     /locus_tag="LAF92_001458"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBI9790486.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            27299..28495
                     /gene="dpaL"
                     /locus_tag="LAF92_001459"
     CDS             27299..28495
                     /gene="dpaL"
                     /locus_tag="LAF92_001459"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBI9790487.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            28553..29764
                     /locus_tag="LAF92_001460"
     CDS             28553..29764
                     /locus_tag="LAF92_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBI9790488.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            29817..31202
                     /gene="hyuA"
                     /locus_tag="LAF92_001461"
     CDS             29817..31202
                     /gene="hyuA"
                     /locus_tag="LAF92_001461"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HBI9790489.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            31250..32182
                     /gene="arcC"
                     /locus_tag="LAF92_001462"
     CDS             31250..32182
                     /gene="arcC"
                     /locus_tag="LAF92_001462"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBI9790490.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(32223..33848)
                     /locus_tag="LAF92_001463"
     CDS             complement(32223..33848)
                     /locus_tag="LAF92_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBI9790491.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(33896..34666)
                     /gene="yqeC"
                     /locus_tag="LAF92_001464"
     CDS             complement(33896..34666)
                     /gene="yqeC"
                     /locus_tag="LAF92_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBI9790492.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            34769..35347
                     /gene="mocA"
                     /locus_tag="LAF92_001465"
     CDS             34769..35347
                     /gene="mocA"
                     /locus_tag="LAF92_001465"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBI9790493.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            35669..38767
                     /gene="ygfK"
                     /locus_tag="LAF92_001466"
     CDS             35669..38767
                     /gene="ygfK"
                     /locus_tag="LAF92_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBI9790494.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PSMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQHNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVKKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            38770..40098
                     /gene="ssnA"
                     /locus_tag="LAF92_001467"
     CDS             38770..40098
                     /gene="ssnA"
                     /locus_tag="LAF92_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBI9790495.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            40149..40928
                     /locus_tag="LAF92_001468"
     CDS             40149..40928
                     /locus_tag="LAF92_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBI9790496.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            40925..>43593
                     /locus_tag="LAF92_001469"
     CDS             40925..>43593
                     /locus_tag="LAF92_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBI9790497.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIG"
BASE COUNT        12638 a         9958 c         9154 g        11843 t
ORIGIN      
        1 ttccctcatc atatcctagt attatcagtt agttaaacac acaaagagat ctgaacatct
       61 taaataagac tatttaagat gcataactta gattcgcaag atatcttccg gtcattcaaa
      121 aacaatttgc gatatagata ttctcatacc atgagatagt tatctgaacc accctcaacc
      181 aggtaagatt aaatcagtga aacataatca tatttaataa cgagaaatgc attttaaaat
      241 gtcttaatca gaattatagc aaatacagat taatcgcaat ttttcccagc aaaattacaa
      301 aatcatggtg tattgacatc atcaatcacc aatgaaatta tgcaattata tacggatagg
      361 gaggttctta acatggggct ttgtagtcgt tataaaagtc ttacatgcaa tagttgctca
      421 atgcattgcc aaataatgcc agaagagtca ccgcgtttgc agtactgcgc aaactcgtgt
      481 ttttgtatgt ggcccgaaga aagttcatat tttaatcgtg gtgtggtaga aggcatctta
      541 acaaaaaacc acaatgcgag attaagcgga tatatttttg tcgatttttc agtaagtttt
      601 ttacgtctat tccttgaaaa agactggatt gactatcttg caagtactga catggggatt
      661 gttttagtca gcgaccgcaa tatgcaatcg ttagctaatt actggcgaaa acacaactca
      721 gcaatatctg ctgtcatata taatgatgat ggtcttgatg ttgccaatga aaagatcaga
      781 caactattta ttggtcgtta tttatcattt accagaggta acacattaac tcagatggaa
      841 ttcaccataa tggggtatat ggtttctggt tataatccat atcagattgc tgaagttctg
      901 gatatggata tccgtagcat ctacgcgtac aagcaacgaa tcgaaaagag aatgggtggt
      961 aaaataaacg aattatttat tcgttcacat tcggtccaac attgatactc aacgaccagc
     1021 cagaatcata ctctgtttat acgggcattt attgaaatgt ccgtatttta taaataggcc
     1081 gtcccctcaa gagtaaacac catagtattt aaaaccatca cacgaaaaat tcagaagcat
     1141 tacgaaatat gaatttacaa acaatagtgg cataaatgtt aaccatgtta atttacgtaa
     1201 agttttacgt tgcaacatta aagcctcatt ttaatcatca tgataaatat aaaattaata
     1261 tatatttatg ccgtaaatac caatatactt agcaaactat gtgatctcca tttcgattga
     1321 tttagtgttt attgacgtat gtactggatt attaacgata atatcgagtt ctggcctgag
     1381 caccgaaaat taatatcggt acataacgcc gatcttaacg tcgttctgac aacgccagcc
     1441 agtcgatgtt tatcacttct acttgaagct tttcctgatg tggttgcaca acaagatttt
     1501 ttcaccagag tctgggaaga agaaggtatg cgtgtgccta ctaacacgtt atatcagaac
     1561 atatccatta tcagacgcgg atttcgcgct gttggtgata ctacccactc gctaattgca
     1621 accgtgccga gaagaggatt caagatccat aatgacatta acatacaaaa tcatgtaata
     1681 aactcgtcaa cagacgcaca tacacacaat gccccacctg ccataaaagt taatgcggga
     1741 tacaaagaga gcattggtgg cgcaaagaat ttcaataaca aaatcctcaa acatataaaa
     1801 agtcatctaa ttatgttgag cgcatttgtc ataggcgcat attctgcata ttggctatgg
     1861 aataataatc aaccgaagcc atttttcaag gattacaaga ctgttgcgga aattaatggc
     1921 tgccatttta atgtaacaga agatacaatt gatgggttga aagagttcga taaatataaa
     1981 acacggatac tggattcggg gattaactgt aaaaaacatc cgtggttata cttccctctt
     2041 gcaaaatcct caccggggat gattgttatg gcatgcaata aaaactataa ccaacatgaa
     2101 gtggcagact gtttaaccct gtcttaccgt gaggttaatc gtgattgatt ataaaaaaaa
     2161 cttattattt attttagttt ttatcagtgg gtttattctc ttcactgtct attcttacac
     2221 tgcagaaaaa atgatatata acgaaacatg cactgcaaat tgggtaattt ttaatgacca
     2281 gggacgagca aatcttacca tcgactttat gtataacaaa aaaaataaaa caggaactgt
     2341 tgcgttgagt ggaacctggc aacaaggtaa cagagaaagt aaatcaatac ggcgaaatat
     2401 tgaatacaca tggattgaaa actatgacac agcccattta acatcaaaga aagttaataa
     2461 gtttgagatt atggatcaag ttgatgatga tagacttgca caacttattc ctgattttta
     2521 tgtctttcca gaaaaaagtg taagctataa tatactaaag caaggtaagc atgcttttat
     2581 tttgagcatt ggtaacagag caataatgca ttgtgcaagg taaactaaaa tagataacaa
     2641 aaagatggaa cctcgttaat cataaaacaa taaaattaaa tattctcgca gtatatggca
     2701 gtctaaagca tcaaagtttt gatcaacatc tttcatttta gacatctcct tgcaatgtaa
     2761 aatatcatgt atcaacaaca tctggtgcaa atccatgagt cgaactcgac attcatctta
     2821 gctcgattat tattccttcg tacagtcgat gtaaacaata cagaaagagg attattaaga
     2881 accagtttac tcagtgaatc aatagaggaa aggactaacg tttctttaaa agaattgatt
     2941 tcatcatctg ttaaactaaa ctcatcattg acagatcgtg agatataact gtttttaact
     3001 ttactcttta cgttgacttt attgacagaa ttaacattca catatcttga ttttaatgtc
     3061 catgatgttg tttgcgtgag aacattctca gcattaagga atttttttaa cggaatcctt
     3121 ggtttctttt tcgagtccga atttacaata tcatgcatat agaccatttc atgaatctgc
     3181 gaaaattcaa tgtccattgt atacctcaca tttttaccgt gactcgatgt tactgttcaa
     3241 taatcacctt ccatcaatac taaaattaat acccctaatg tgccgataac aaatatagtc
     3301 attctacgta acgtctccat aaggtgatat ttgacattat cagaagttgc gaattcggat
     3361 tttgctctaa tcaaatgaga gaaatgtaaa atgaagccac gaaatattaa taatagccta
     3421 ccactgcaac cattagttcc tgatcaggag aacaaaaata agaaaaatga agagaaatcc
     3481 gttaatccag ttaaaatcat aatggggtct ggtttaaatt atattgaaca agaatctctt
     3541 ggaggaaaat atctaacaca tgatttgtca ataaagatag cggatatttc tgaagagata
     3601 attcagcaag caatattatc tgctatgagc atatataaat tttcgataac agatgattta
     3661 atgagtatgg ctgtaaatga actcataaaa ctgaccaaaa tagagaataa tgtagacctg
     3721 aataaattca ctactatatg cacagacgtt ctatcccccc gcgtcaccag acataataaa
     3781 gaaaaaaaac aaacgacatt ctacccttct caaaaatccc ctttttaatt ttcattgaaa
     3841 aaatggcaat tgatactatg aatccaggtc atacacatta aggcatttat gtaatgtcat
     3901 aattaagata actaataagg tgaatattag taaaacaact aatgatgtta ttagtttgta
     3961 gtgaacagta cttttaccaa taatgaaaaa tataccataa gcaatgcaag cttaaaactc
     4021 ataatttaaa aaagataaat ataaaaatat caatgagtca atcaataata attttgcatc
     4081 acgagacaca tcacagagga atattatgag cacagaaaca attgaaatat tcaataatag
     4141 tgatgaatgg gcaaatcaac taaaacacgc attatcgaaa ggagaaaatc tggcattact
     4201 acatggttta actcctgata tccttgatag aatatatgca tatgcattcg actaccatga
     4261 aaagggtaat ataacggacg cagaaattta ttataaattt ttgtgcattt atgcgttcga
     4321 aaatcatgag tatctaaaag attttgcatc agtatgtcag cccaaaaaga aatatcaaca
     4381 agcatatgac ctttacaaac taagttacaa ttactccccg tatgatgact attcagttat
     4441 ttatcgtatg ggtcaatgtc agattggggc taaaaatatc gataacgcaa tgcaatgttt
     4501 ctatcacatt attaacaatt gtgaggatga tagtgttaag agtaaagcgc aggcatatat
     4561 tgaactctta aacgataatt cagaagataa tggctaagca ttacaatcat ttggttaaat
     4621 ttgctggtta tagtgcagtt aaccactata accagcacct cttatgtttt cttaaatcag
     4681 catcttaaaa cagttaagag catactataa atcattactt ttcaatgcgt tagagaacaa
     4741 tctactttat aaaaattgaa taaggctatt tcagattaca tcactgaatt cctaagataa
     4801 attagtgaac acaaccatag atatgtcgaa tgtaatttcc tgtagcatta atgacacata
     4861 aatatgaata gccataattt ctattgctta agcaggatgc aagaaaccaa ttttttcata
     4921 gaggttaact aatggactta gaaaataaat tctcatatca ttttcttgag ggattaacgc
     4981 tcacggaaga tggaattctt actcaaggaa atgagcaagt ttatattcca cagaaagagt
     5041 taggtgtatt aatagtatta cttgaatccg ctggtcatgt tgtactgaaa gatatgatca
     5101 tcgaatcagt atggaaaaat attattgtta gtgacgagtc cctgacaaga tgtatctatt
     5161 ctttgcgctg catttttgaa aaaattggct atgatcgttg catagaaaca atctaccgga
     5221 aaggttatcg tttcagcggg caggttttca aaactaaaat taatgaagat aatacttcag
     5281 actattccat agctatattc cctttcacta cttcattgaa tacactggat ccattaatac
     5341 ttaatcagga attagtgcaa atcatttcaa ataaaaaaat cgatggtctc tatacctatc
     5401 cgatggctgc gacaaatttt tgtaatgatc acatatctca aaattcattc ttgagcagat
     5461 tcaagccaga ttatttcgtt acaggaagaa taaaccagaa taatgcagtg aacactttat
     5521 acattgagtt gatcgacgct aaaaaccttt tcctcatcgc cagtaatcat ctccctgttg
     5581 atgaactaca taatacatca caatttatta tagataatat ccttcaaacg gtacataaac
     5641 cagaacgatc tgtaagatta gctaagcagg accaaggata taagaatcat tatttatcag
     5701 atgaaatgtt agccggaaag aaagaacttt acgacttcac ccctgaaagc atttacaggg
     5761 ccatgactat atttgataga ttacaaaata aaagtgatat acagacgcta aaaacagaat
     5821 gttattgcct tctagcggaa tgccatatgt ctttggcact tcatggaaaa agtgaacttg
     5881 aacttgctgc tcaaaaagca ttagagcttt tagattatgt atcagacata accactgtcg
     5941 atggaaaaat tttagctatt atgggactga taactggtct gtctggacaa gcaaaagtat
     6001 ctcatatctt atttgaacag gctaagatac actcaactga tatagcctct ctctactact
     6061 atagggcact agtccacttt cataatgaaa aaattgaaga ggcaaggatt tgtatagaca
     6121 aatcactaca actcgaaccc agaagacgaa aagcagttgt gataaaagaa tgtgtagata
     6181 tgtatgtgcc taacccgctc aaaaacaaca ttaaactcta ctataaagaa actgagagtg
     6241 aaagccatcg agttataatt gacaacattt tgaaattaaa gcagctgacg agaatttgta
     6301 tgcgataaat ttttattgac gcaattttat tatttagatg tatgcaactt gttatttaaa
     6361 caacagagat aaaaatatct aagatattca ccttattgca agatatttaa aatgctctag
     6421 agttaaaagt atgatgtaaa gaccattggc ctggacaatt aaaaaaggga gctttatgac
     6481 taatccaatc ggtatcaata atttatctca aagttcaaat atagcgaatg caacgggtga
     6541 tgaagtcgta tcattagata aacacatcaa caccagtgct actgatacag atcaaataca
     6601 agcatttata gtttcaacat ggatggcgcc ttttcaaaat gatatgtatt cagaagataa
     6661 tcctatctca ccttactata aaattgaatg gtagacgatt gaccaaataa atcataacga
     6721 gatactttta taatagtatc tcgttccttt cattcaatcc tcacacatga aaaaatatgc
     6781 gctcaccaaa agttaaattc ttaaccatct tcacgttttg tatatttatt acaaaaatgt
     6841 ctttcgccag caactcttgc tctaatgaag caggaacaat gttcagaatc gaaccaaatt
     6901 taattaaagc tatcgctctt gttgaaagca atctaaaaaa ggatagcata ggcaagaaca
     6961 gagataagaa taacaacata aagagtttag attattagtt aatgcagata aaccagatgc
     7021 atattcccct gttaaaaaaa cgtggaataa taaaagatga acgagatttg ttagataacc
     7081 cttgtctgaa cattaaaata ggaaccgaga ttttatataa ccatttttca cgttgtggag
     7141 taacgtggca atgcctgggg acttataatg cgggttttgc aatggataac caaaaaaaga
     7201 gacagcaata tgccccaaaa tatatattgt atataccagg cttaatgaac tagacaatcg
     7261 taaggcttca gcaaaatgag cctaaagcct ctttttttta tatagtgcaa acacccatac
     7321 gtaaagctgt tttgatcaac acgataccac tgtgaacatc taatttcttc atgatattga
     7381 gtctatgagt ttcgactttt tttctgctta actgcaagaa attagcgatt tctttgtttg
     7441 ttttaccatc agctatgagt tgtaatattt ctgactcacg atttgtaagt tgattatcag
     7501 aggaatacct ggaagatatt ttttcacaat ccatatgtac actatcaaaa taagtgtaac
     7561 cattactaat cgaatcaata gcacgattta gtttcgccgg gtgactggtt ttccatacgc
     7621 atcctttcgc acctgcttca agcaatttta ttgaatcaat atacggcatc tttgctacaa
     7681 atgtaataat atgagcatct atcttatgcg atttaaccca ttttacgagt tcggacccca
     7741 tcattccacc tgcaaattct cctaaaataa atatttaggc tgtagatttg ttacactccg
     7801 ctatagcttt cttcagatct ttatagcccc caacaacctt atataaatca ggataatgtc
     7861 cgagaaatcc aattatccca tcaatcataa acggctgctg atctgaaact acaattttaa
     7921 tttttcccat atattcatcc tgagtgtaaa tgctttaacg cattcattta taatcatccc
     7981 ttccattatt attataagca aaatccaaag aatacattga tgaaataata atgaaatata
     8041 attaaaaata aaattttttc gtaaaaaata ccacaggcat taaaaaatca tgagatgatt
     8101 aaaatattac aattagatta tattcaaatc attaaacttg agccagggag ctataagtcc
     8161 tcagggcttg ccaaaacaga attattcata tccaaagaat gtgtttcaaa cattttttca
     8221 actaaataat gtctggcttg ttcgcagatt tcgttaacct tgtcagggga gaaacaatga
     8281 ttattaatta aatattgctc acaattatcc acaaattcct gcggactaaa ttcagcaata
     8341 aattgatcgg taaaaaacac aaaacgctta tcatcatgaa atgtaatatg gacattccat
     8401 attgatttgt cgacaaaata tgactctact tcaagatact tatctttgaa tctggtaggt
     8461 ataaagagat ttacaggacc tgatattgcc ggtaattttt ttctccaact ttcaaaaatt
     8521 ttgataaaaa catcaacttc ctctgaggat tttaacaata tactcttcag atcgtttatc
     8581 atgtttattc ttttttttct agttctgagc tcaacaagct tatcaagggt ataaaattat
     8641 ccatcacatc aataatgcct tttgtatagc catcataata accttgaata aatttttctt
     8701 ctctctttac ctcaatgctc tgaatttttg cgctggtttc aagaaggata ctttctttaa
     8761 gagaaactat ttatttcaat ccatcagaaa tttttctttt aatcacaaca ccatttgcag
     8821 gagattttat caagctcatc tctatttttt ttcgcataaa atgtaattct ctctaaaaga
     8881 gttgatgcaa gtggtaacgg caattgtatg tgatcaacaa aatcaggaaa taataaatta
     8941 aatcgttgct gcattgccaa tggttgttgc cttatatagg gtgacaacgt tgaaaaacca
     9001 catgtgatga tattgtgata gctagcgatg ccgggtttgt atcgtgcctt ttcatttatt
     9061 ttcaccggta tagctgataa ataatccctt agcacagcag gaaccttata aaaaaatccg
     9121 cgttctgcaa acctttctcg ataaaagtga taaccactga ataaacagaa caatggaaat
     9181 aaattccaat tgtttatgta gtaaataagt gaactattca gattaaaatg ttcaactgaa
     9241 agattgtact gcagaacaat catttcattg gtaatagatc taagcaccgg attgttaata
     9301 ggtgtattat tcaagcttac tcgttgagga tgaatataac taattggatc atatataatc
     9361 tttcttaggg caagattcat aacgctctca tatgtctact taattttcaa cctgactaaa
     9421 ttagttagaa tggccctata cttccataac agccagcaag tcgctacgga tattaatgca
     9481 agtaagatag aaaccggcat agccttatca taagcaaaaa caggttcgct aatttcatat
     9541 gttggtgctt gctcaataat gtctcttcgt tttgacaata caacagaaat attttcatat
     9601 tgtacgcttg cagagctatt aacaataaat ctcttgatat catttatttt tatttctggg
     9661 ttgatatctt tttcatatac tgcaagtaca gaaatatgaa ttggtaaagc agttttacca
     9721 ctatcgccat tatcaacatc gtaactaaca tgtactctcg aagaaataat gccatccata
     9781 attttgagag attgctctaa ccgctgctca atagcagaat atagccttgc tttttccgct
     9841 cgtggagatg aaacgagtgc atctgcaggg aacatctgcg atatttgaat atcaggctta
     9901 cccggtagat tgtagatttt tagccaatcc accgcagaag caaaatccgt tggttcgaca
     9961 aatattgaaa atcctgtttt gccttaatcc ttcttttcag cattaatatt atgtctttgt
    10021 aaaacagcaa ggacatcatt agcttgctgt tgaacaagat ggttcaataa ttcctgctgc
    10081 ttgcaggcgc acaacagcag gataaacaat aataatgata tatatttcat gagcgggtca
    10141 acgtctcaac tgcagcgaca atctttcttg ctaatgttcc accaatgaaa cctcgacatt
    10201 ataattaagc acatgatcct gcaagactaa taatttttca ggagttaacc cctctttatc
    10261 agaccttaat gcttgtaacg cattttgctg ccaattttca gtttgtacag catagttggc
    10321 aaaagcggag atcattttat catccgggtt gtcaagattg agaacccggt catttaaatc
    10381 aatcactttc gcttgctgta tttcatgagc agttgattgc tcagctctat caataggtgg
    10441 catattgctt actgacataa taaaatcctt atccagttta gcggaagttt tgtatgattg
    10501 ctgaatcaag atctttatac gcttttacaa cgttagattg cgcatttcga tataatgtat
    10561 actcagctaa tgcactttga tattcggcaa gaaatttcgt gtcggaggga ttggttggct
    10621 aaatcctcca acgctttttc aacttgctgg ttcagatcat caacgccctg atcaaattga
    10681 ttgctgatat ccataatata accattccaa tctgccataa gtcctcctca attattcctg
    10741 aaatgtccag ttgctacctt gagacttctc gaatcggtta tctccgtagc taaatgaact
    10801 attatttgtg tcctgatttt tcaaactcac attaaatctt acatattgtt taccctattg
    10861 tttataatat tcgtcaacat agttattaat tttatttaat tcactgtcac ttaacgtacc
    10921 aatgatattg aaatacacat tgttagctgt tttatatttt tcataagaaa gacctatcgc
    10981 tgacaacgtt tcaatagcct gactttccaa tacattatca tctaataccg caatacttat
    11041 attgctggca tatggcatag tttgcagtaa tcctttaata agattatcga ttagttgctg
    11101 tgtgatagcg ttccgttgct tactcactgt caatcttaga ttactggcat ctaatagttg
    11161 aagtttaaaa tattgtaaat tagggaaatt atcagcaagc catgaaaaaa ttcgtatcga
    11221 ttcatcatca ggatttatca cccgtactgg ctttcctggt tgattttttt ccagtgcttg
    11281 tttgacccag actgcttcat ccaaaatatt ggtattaata tagataacat gatctctacc
    11341 atataaaact tcataattcc tgtttttgtt tacaagaatt ttatcgatat gtctcagctg
    11401 cggatcatta ctagcattaa caatataaaa agcaataaaa atgatagcaa aaagaaaaaa
    11461 aaagcattac atacttaaac agcttactgc taaagaaatg ttgttttgta gaaataatgt
    11521 tattttcaat aatgcctggc aatgaatact gccaaacttc atttttttct tttacagcaa
    11581 atttgagacc taaaacttct atttgctgat tcaatgttaa cgttctatct tcaggttctt
    11641 gtgctgttaa ttctctgaat gttactacca gatcagggtc ggtagtaatt attatctcaa
    11701 aactacctgt aagctcgcca taaggaacga ctattgtgtt ttctggtaac tcagaaaaag
    11761 catcacttct tcctaacgaa acatcattac caatgataac aagtagacgc ccattaagta
    11821 tttcatactc gcatccattc aatggtccgc tcaacaaacg aatggcataa gattccaata
    11881 agtcttgtga taagaattta tcattatttt ccatgaatac cacccgctaa ttccataagc
    11941 tctagtttag agtagtgtaa caagacggaa atttcatgac caaattatga tgacacagaa
    12001 taaaaaaatt atattttcga gaactatttt aaatgcctct atgcataact ttatttacaa
    12061 tttaaggtat aaaccctatt ccctcaatgt taagcaaata taacaatcac tcttatttta
    12121 aattttacac gtaatattta ccctaaatga ttttagagta caggtgggca tatgcaagtc
    12181 ttttccagtg atgtctactt cactgtaggc actaatgcat tattagcatc ccaaaaagaa
    12241 tattacagcg atttagttgc gttggttgat cttggacatt cgttcgttgt tattgatgag
    12301 catcaacatc gaaatctcaa accaaataca gaacctgtaa acatcctttt gagcaacaat
    12361 ttcatcagga tcaataagaa tattacatta tctgatttaa cgcatttcct ggtttcaaat
    12421 cttcatacac aaaatgtgta ttccacacaa gaaccactta cccatgatga gatagatatc
    12481 ctgcggttat gtgtttctta ctcgttaaaa caaatagcca ttattaaagg aattgattat
    12541 aaaacaatat catatcacaa aataagagcc ctaaataaac ttaacataaa aggtactgtt
    12601 tagttgttta tagcactctg tgaatgggat aaacattatt ttaaactcca atcttgcgta
    12661 agagaaagtt gatatgtcat tagtaggata taatagtaac atcataataa ccatcgcgct
    12721 gatatcgttg ctctctgcat gcaccagaga aaattcatta aaggagaatc aaaataaaag
    12781 aagtggcgaa tcccaagcga tagtagatgc aagacagttc tttagcgaac acccggaata
    12841 tatatcatca gcagaactgg agcaaaagct atatcgcgaa tttgcacagg ttactacagt
    12901 tccagcttat aaagagatga gtatgtatca attgcttatc atttcacaag atcgtttaac
    12961 tcattaaata aggatctaca cgctttttgc gtgtagattc ttataaattt ctgactacga
    13021 cagatgttta attttttaaa ttatcgtctt tattctcgat ttttgtgtct tcaccctcaa
    13081 tgaatttatc ttctgaagag agctgttcat cttgaacagg ttgcccagca ttctccacat
    13141 cttcaagcca cagcagaaga cgtaatattt catctatatt ttcgaagcta acataatcgt
    13201 agcgacgatg ggtagcataa ttttttcgtg ctaatttttt atctgtgata attggtattc
    13261 cgatttcctt tgcatatttc cttacgccta aggccacctc attagttact cttacagaaa
    13321 tcaatggaat aggtgaaaga tgtggcttaa agtatatccc tattgcaatg tgagtagggt
    13381 tggaaatcat caaacgacta ttactgacat cagacttcaa ttgctcagaa agaatttcct
    13441 gatgcgctct ctgcgtttag acttaatttc tggatttcct tcttgctcct tgtattctct
    13501 tttaacttct tgtttatcca ttttcatatc tttcataaat aaaaaatatt cagcgataaa
    13561 atcaaaaatt aagacaatta tcatactgcc gagacaatac agtatgagaa ggaatagcat
    13621 ctctccccaa tctgataata aagatatgat atctccatca agagttttaa aaagcagtga
    13681 tttatgatta ctccaaaata ctttgatcgc caatgcaaaa aaaacaatat aaagaattgc
    13741 tttaacaaat tcttttacgg tttttaagcc aaatatacgc tttaagccat ttactggatt
    13801 caatgaatca aacttaaatt taacagcttc agttgctaat tgcattttac tttgaaacca
    13861 ggagacaagt attgttgtga acatgcaaac cagcaaaaaa ggaatgttcg ttttcaacca
    13921 accaaccccc atagctttaa aatagtccca aatatctggt ttagcgttat gatcaaggat
    13981 gtattcaaga atcgacataa tgtgatgcac atcaaaaaca tatccgagat ataaagtacc
    14041 cacaagcatt attacagaga tggttaagtc tctacttttt agaatttgcc cttttttaga
    14101 agcatcctgg agtttctttt gtgttggctt ttcagtttta ttggccatga aacactctat
    14161 ttctcaatgt atagttgcaa ttgttctggg aaaaaggata acggcattac tcgttcagcc
    14221 agtattgggg caaaataaat aattaatatt aaaaatgcca gcgcactttt gacagtcagt
    14281 gaaatggcaa aagcatttag ttgtgaagca taacatgcca gcaatcccag gacagcctca
    14341 ccgcccagca taacggcaat cacggggctt gcgtaaacga tagtatgagt cattatgtta
    14401 ttaatatatg agaataaagg ctctaattta ggaaaattaa aattgcctga aggccataaa
    14461 ttataacttt gccaaagagt ttccagaata aagttcattc cacctttagt aagataaacc
    14521 gcagcagaga aaaggttaaa taatcttgct aattccgagg tatcgacacc tgtagccgga
    14581 tccaaagtac tacttaatgt tgcaccgcgt tggttatcga taaagccacc tatagcaagg
    14641 aaaatccaaa acggggatgc taataagcat gcaataaaca gccctatgat aatttctctg
    14701 gcagcgaaca aagcaatatc gatttcattt aagttcaata attcatagtg gtaatgctga
    14761 actaatgcac atgataccac tattattatt gggtgacgaa ctactgttgg tatattccca
    14821 ctggcgaaaa atggtaacaa atagaaagtt ggtgccaatc ggcaaaaacc gagcgctgta
    14881 gcagccagca gactatgcaa ctgatagagt atcgcctccc ccatgctatc ctctcgcgaa
    14941 tgctaaattc agcatttcga taccaaatga atataatttt tcaccatacc acccgctcat
    15001 taaaaaaaag caaatactta cgcacaataa tttcacgcca aatggcaacg tctgttcttg
    15061 caactgcgtt accgtttgaa aaagccctac caataaacca acaaatgtcg caactgcaat
    15121 aggtcctgca gacataacta aaatcagata cagcgcccta ttccccgcaa aaacaatatc
    15181 gtccatattt tttcctcagg gattaatgga taggtcaaaa tattgtaaaa tgagcccttt
    15241 tgataacatt gtccaaccgt ccatcgcgac aaacaaaatt aattttatcg gtgtagatat
    15301 ggttaccgga ctcatcatca tcatacctaa cgttaacaga acgctggata taaccagatc
    15361 cactacgaca aagggtaaat agatataaaa acctataata aatgcacttt ttatctcact
    15421 caatgcatat gcgggcagta acgagaaaat tgaaatatta tcatcatcga ttatctcttc
    15481 attgtcttca gaactattca ccttctgaat cttttcaaaa aagctaacca actcaggttc
    15541 agaatatttt atcaaatatg atttataacc actcatgcct gtctcaacaa aattaacaac
    15601 cgatgctaca ttattgaatg aaagattctc attctgactg ttattatata tttccttccc
    15661 aaccggcatc attacgaaca ttgacaatag caaagcaacc ccattaagtg tcatatttga
    15721 tggcacttgc tgaagtccca gcgcgtttcg gacaataaca aaaacaatcg agaatttaat
    15781 aaaacatgtc ccggatgcta ttataaacgg caatagtgta aataatgata atatcgctat
    15841 cagcgaaatg ctatttgaca ttattattcc ctgataacca agaatgaatt tctacgccca
    15901 atttatcatc cacttcaaca agttcgccat agccagttag cgcaccattt acacgtatct
    15961 ctatattttt ctcagattca ggaagcagag atattattct ttttgcacag agatcgtcta
    16021 tttcataaag attaattatt ttcttaccaa gaacaaactc aatttttaca ggcaactctt
    16081 caaaactatt aatcatctgt tcgtcatttt catcataaat ctcactctct tttttttgat
    16141 atcaaaatca tctgtttcaa aatcttcctc gtactcaaaa tgatcagcca tttttaactc
    16201 ctctggataa attaaatcac aaatttttgt attataaata accgcataag ccaaattatt
    16261 tgagattaat aaaacatcac caacttcaat atcaacaaga cttctgtaac atgttttact
    16321 aaatccaatt acaaaatgaa tggaccaata taatgatttt ttgtctaaca ctggagcgtt
    16381 tccgtattgg cagactgtga ctttatctaa ccaaagattc aattcccctg ttcatattct
    16441 tattagtggt tgagcagggc tcaatactcc atcaaatact ttacctacgt gcaaacgatt
    16501 atattttagt tcaggatgct ttagatttaa cggggaactg gataaactga gccattcccc
    16561 tattaacctt tccgtttctg gcattaaagc catcccagcg agctcagatg aaattaaggg
    16621 aaatatttct ccaagcaata cccatccaga ccagctgcca ctctcttctg ctatcggtaa
    16681 ccatttagca ctaaattcgg gcattaataa tgcaacatct tcgccatttt ctttccaatt
    16741 ttgaaaagtg gtttcaaacg tatgagtgtt tcgatttact ttcctcagac cgaacattaa
    16801 ctttcatcct tctgatcgtt ttcgaatttg ctttcatttt cttgctcctc ataaggctga
    16861 tgtcgttgtc cttgtcgttc atcttgctca gtcaataccc aacgttgtgc tgtaactcat
    16921 tatgaaaact atttaattcc ctcatcattg ataaggttgg acatctcttt caacatgaga
    16981 gtcattgaag ctttcgacca atttagcgga atgatagggc ttggcgcttc atgcaaagtt
    17041 cccgacttgt ggatatagtt atagctttcg ggaagtgcca tttcaggtac actcctgcca
    17101 tttgcaccaa tcatattctc gcctgttatt tgggccaatg atctattcat aaattgcact
    17161 gagtcattca aatactcttc ctttcttgat tctttataaa caattgctgc tgattttgca
    17221 taccatgagt caaaaaacca ttctgcttct gttgatggaa tgaagctctt ttctcttttc
    17281 gcatgtgaat tttgatcggt atcggtttta atatcattga accaaaaatt tgccgactga
    17341 taattatcct tctcgtatct ttttatacca tacggacctg ctagtttttt aactattttt
    17401 agaacctgct cttttcttct ggtgtttatc tttgcaagat ttgcaggata aataacatta
    17461 agtaaagccg catcggcttc tctgtaatgt ttatcttttt ccagataacc tggggattct
    17521 ccccctaaat caagctgtaa cgttattctt tcataacctt tatctatgag gtggttcaac
    17581 ctcgttgttg aaagaggttc atccagttca ttggctttag cttcgcgtag taaatcagag
    17641 acaaaaacag agtctttctt cgagagtaag ttcgacaaac gttccagtcc agaggtcacc
    17701 agtgcaacgg aagatgtgtt taatcgggca tcttcttccc atgctccgga atcttccata
    17761 acatagaaat tggctttatc cagataagca atcagcagcg caaccgattt taatcggtca
    17821 cctttttgcc aatcttcagc gttaattgta tcagtgtcga ttgcttgtat aaggagatca
    17881 agataaagac ctaacgcatc attttgttta tggttccaca gctgcggctt gccttcttcc
    17941 tgcacatctg ccatcacggg ggagttgctg tcaaatcgga tatgcacaac attcatttgc
    18001 ccgggaactc catccagtcg tttggggttg ctaatgacgt cctgcatccg cttaatctgg
    18061 tcgggcgtag acatataatc ccacagggtg agcagaacct tttttgcggc tacactattt
    18121 ccctgatctg atactaatgc catatagccc cataaactat ccctcagcca gacagcatca
    18181 tagtgggttt ctgccgctgt tttgtcatct tcgaaagtag aagcaagaat aaacgttccc
    18241 cagggtttct ccgtcaacgt taactttcgt gtgttttctg ttaatgttag caatccttga
    18301 atttcgtctt tggtatagtg gctctttatt tcattttgta atttggctga gtgaatcaaa
    18361 agacgataat taggatactt atctggattt aacgataaat aacttttatc tgttgctgcc
    18421 cccgtggaat taagaatgtt aaatacagat tcactcgtca cttctgcccc tgtcgcctgg
    18481 catgtgccgc acgaaaatat accagcgagc agaagagtta atgcagattt ccctttcata
    18541 aaatattcca tcaatcgttt gttaaaagcg tagataacca cttatttaac aacttgaaaa
    18601 taaggtgttt tacctgggtt gttacaaaag gattgcattg cgtaaacgct ttttatttac
    18661 aacaaaatgg ggaagtatta cggcgattgc gaaaagtctg aagcgattga attaagataa
    18721 tatagaaaat cagatacagc aaaaatggca gattgatcag gtgaagataa attggagcgg
    18781 gcgaagggaa tcgaaccctc gtatagagct tgggaagctc tcgttctacc attgaactac
    18841 gcccgcttcg agatgcgtaa ggcattataa accttacgct ctccttagca agtgccgcgc
    18901 tgctgactgc tgattaattc gccatcagca ttttggcttg ctgccctgcg gcggcaagta
    18961 acgtagcgga tcaattgccg ttgcacggta acgaatctgg aaatgcaggc gaacagatgc
    19021 cgcatccgtg ctccccatag tggcgatttt ttgcccagcc ttcacgcttt gcccattatt
    19081 taccagcatc gtgtcattat gggcgtaagc cgtaatgtaa tcttcactgt gtttaatcat
    19141 gatgagatta ccgtagccac gcagctggtt gcccacatac accacctttc ctgcacccgc
    19201 ggcgtaaata ggtgtacccc gtggagctga gatatcaatc cctttattgc cgccatctgc
    19261 tgtcgaatac ggcatgataa ctttccctgt cgttggccat aaccaacaac gttgccctac
    19321 tggcggccag gaagatttcg gtaccgctga tgacggtgta accgatgcgg ttttggtcgt
    19381 tgatttggcg gttgatttac gtgtactact gctacttttc gccccaccca gttttagttt
    19441 ctgaccaact tcaatggtgt aaggggggga aatgccgttc agtcgcgcca gttcttttac
    19501 gctggttccc gtggtgcgcg aaatacgata tagcgtatcc ccccgtttca cggtgtaaac
    19561 ggagccggaa tacgttcctg tatcggatga tttgctaccc gaacagcccg ccaaaagcag
    19621 tccaaccgat aacaacatca cgatacccag agatttttta ttcaggcgtc ccgcactcaa
    19681 aatctttcct cgcttaaaaa acaagacgcc ttatcatagc agccagcctc ttgataccca
    19741 atctcttgct tctggaaaaa cgtggtaata gagaaagagg ttaatttcat ttggcaaagg
    19801 gaagtatcat cagcaaaata ttcgctgtag aggtatcgaa cttcctgact ttttacccta
    19861 tttcccatcc tgatgcgtaa taatcccaat ttatcacatt gattttatcg ccactcatat
    19921 tgatacgtat cactgcccct attcagagtc ctgtttcgta tcatatctgt gtttttttaa
    19981 cgtcatcaca cttcctacct cccctaacgc ttatcgtcgt ttctggcgta aatcttgcct
    20041 gcttagacta aatctttgcc ataagaacca aatgttcaaa tcgaggggat ttctatggaa
    20101 gcgcgggaag caaccgctac gggtgaatca tgcatgcgcg tcgatgccat tgctaaggtc
    20161 accgggcggg cacgatatac tgacgattat gttatggcgg gcatgtgtta tgcgaaatat
    20221 gtacgtagcc ctatcgcaca tggttatgcc gtaagtatta atgatgaaca agccagaagt
    20281 ttgccgggcg tactggcgat ttttacctgg gaagatgtgc ctgatattcc atttgctact
    20341 gctgggcatg cctggacact tgacgaaaac aagcgcgata ccgccgatcg cgcactgcta
    20401 actcgccatg ttcgtcatca tggtgacgcc gttgccatcg tcgtggcccg cgatgaactc
    20461 acggcagaaa aagcggcgca attggtcagc attgagtggc aagaattacc cgttatcacc
    20521 acgccagaag cggcgctggc agaagacgct gcaccaatcc ataacggtgg caatttactg
    20581 aaacaaagca cgatgtcgac gggtaatgtc caacaaacaa tcgatgccgc cgactaccag
    20641 gtacaggggc actatcagac ccccgttatt caacattgtc acatggaaag cgtaacatcg
    20701 ctggcgtgga tggaggatga ctcgcgaatt accatcgttt ccagcaccca gatcccgcac
    20761 attgttcgcc gcgtggttgg tcaggcgctg gatattccct ggtcatgcgt acgagtcatc
    20821 aaaccgtttg tcggtggcgg ttttggtaat aaacaggatg tactggaaga gccaatggcg
    20881 gcattcctga ccagcaagct tggcggcatt ccggtgaaag tttcccttag ccgtgaagag
    20941 tgtttcctcg caacccgtac ccgccacgct tttaccattg acgggcaaat gggcgtgaac
    21001 cgcgacggaa cattgaaagg ttatagtctg gatgttctgt ctaacaccgg cgcttatgca
    21061 tctcacgggc actccatcgc ttctgcgggg gggaataaag tcgcttacct ttatcctcgt
    21121 tgtgcctacg cttacagttc aaagacctgc tataccaacc tcccctcggc tggtgcgatg
    21181 cgtggttatg gcgcgccaca agtcgtattt gccgttgagt ctatgcttga tgacgccgcg
    21241 acagcgttag gtattgatcc tgttgaaatt cgtttacgca acgccgcccg cgaaggagat
    21301 gctaatccgc tcacgggcaa acgtatttac agcgcagggt tgccggagtg tcttgaaaaa
    21361 ggccggaaaa tctttgaatg ggaaaaacgc cgtgcagaat gccagaacca gcaaggcaat
    21421 ttgcgccgcg gcgttggcgt cgcctgtttt agctacacct ctaacacctg gcctgtcggc
    21481 gtagaaatag caggcgcgcg ccttctgatg aatcaggatg gaaccatcaa cgtgcaaagc
    21541 ggcgcgacgg aaatcggtca gggtgccgac accgtcttct cgcaaatggt ggcagaaacc
    21601 gtgggggttc cggtcagcga cgttcgcgtt atttcaactc aagataccga cgttacgccg
    21661 ttcgatcccg gcgcatttgc ctcacgccag agctatgttg ccgcgcctgc gctgcgcagt
    21721 gcggcactat tattaaaaga gaaaatcatc gctcacgccg cagtcatgct acatcagtca
    21781 gcgatgaatc tgaccctgat aaaaggccat atcgtgctgg ttgaacgacc ggaagagccg
    21841 ttaatgtcgt taaaagattt ggcgatggac gctttctacc accctgaacg cggcgggcag
    21901 ctctctgctg aaagctccat caaaaccacc actaacccac cggcgtttgg ctgtaccttt
    21961 gttgatctga cggtcgatat tgcgctgtgc aaagtcacca tcaaccgcat cctcaacgtt
    22021 catgattcag ggcatattct taatccactg ctggcagaag gtcaggtaca cggcggaatg
    22081 ggaatgggca ttggctgggc gctatttgaa gagatgatca tcgatgctaa aagcggcgtg
    22141 gtccgtaacc ccaatctgct ggattacaaa atgccgacca tgccggatct gccacaactg
    22201 gaaagcgcgt tcgtcgaaat caatgagccg caatccgcat acggacataa gtcactgggt
    22261 gagccaccaa taattcctgt tgccgctgct attcgtaacg cggtgaagat ggctaccggt
    22321 gttgcaatca atacactgcc gctgacgcca aaacggttat atgaagagtt ccatctggca
    22381 ggattgattt gaggataaca tcatgtttga ttttgcttct taccatcgcg cagcaaccct
    22441 tgccgatgcc atcaacctgc tggctgacaa cccgcaggcc aaactgctcg ccggtggcac
    22501 tgacgtactg attcagctcc accatcacaa tgaccgttat cgccatattg ttgatattca
    22561 taatctggcg gagctgcggg gaattacgct ggcggaagat ggctcgctac gtatcggctc
    22621 tgcaacgaca tttacccagc taatagaaga tcctataact caacgtcatc tcccggcgtt
    22681 atgtgctgcg gccacgtcca ttgctggacc gcagatccgt aacgtcgcta cctacggtgg
    22741 aaatatttgc aacggtgcca ccagcgcaga ttctgccacg ccaacgctaa tttatgacgc
    22801 gaaactggag atccactccc cgcgcggtgt tcgtttcgtc ccgattaatg gctttcacac
    22861 cgggccgggc aaagtgtctc ttgagcatga cgaaatcctc gtcgcctttc attttccgcc
    22921 acagccgaaa gaacacgcgg gcagcgcgca ttttaaatat gccatgcgcg acgcgatgga
    22981 tatttcaacg attggctgcg ccgcacattg ccgactggat aacggcaatt tcagcgaatt
    23041 acgcctggca tttggtgttg ccgcgccaac gccgattcgc tgccaacatg ccgaacagac
    23101 tgcacaaaat gcgccattaa acctgcaaac gctggaagct atcagcgaat ctgtcctgca
    23161 agatgtcgcc ccgcgttctt catggcgggc cagtaaagag tttcgtctgc atctcatcca
    23221 gacgatgacc aaaaaagtga ttagcgaagc cgtcgccgcg gcggggggaa aattgcaatg
    23281 aatcacagcg aaacaattac catcgaatgc accattaacg ggatgccttt tcagcttcac
    23341 gccgcaccag gcacgccgct ctcggaatta ctccgcgaac aaggactgct aagtgtcaaa
    23401 caagggtgct gcgtgggtga atgtggtgcc tgtacggtgt tggtcgacgg cacagcaata
    23461 gacagttgct tataccttgc cgcctgggct gaaggaaaag agatccgcac gctggaaggt
    23521 gaagcgaaag gcggaaaact ttctcatgtt cagcaggctt atgcgaaatc cggcgcagtg
    23581 cagtgcgggt tttgtacgcc tggcctgatt atggctacca cggcaatgct ggcgaaacca
    23641 cgcgagaagc cattaaccat tacggaaatt cgtcgcggac tggcgggaaa tctttgtcgc
    23701 tgcacggggt atcagatgat tgtaaataca gttcaggatt gcgagaaaac gaagtaaaag
    23761 gatatccggc ctgaattcag gccggattca ctgaggttat gtgtttaaca actcatattt
    23821 cttaatcttg cgatagagcg tagcaatgcc gatgcccagt tcatcagcaa cttgcttctt
    23881 gctgttatga cgtgaaagcg cctcgcggat catttgcttt tccatctcct ccagcgccgt
    23941 gccgcccgca tcatcgagtg acaggtgcgc ctcactgacc tctgttacat cactttgctc
    24001 cgttgtgcca ttattcagca gatttggcgg caatagcgtg ctgtcgataa cttcacctga
    24061 aggaactacg ttaaccagat attccatcaa attgcttaac tcgcgcaggt ttccgggcca
    24121 acgatgctta cgcaatattt cgacgacatc gggagcaatg ccaggataaa ccgatcccag
    24181 acgacgggta tgcagatgta aaaagtaatg caccaatagt tcaatatctt cctgacgttc
    24241 acgcagcggt ggcagagtta tcgggataac attaagtcgg tagaagagat cttcgcggaa
    24301 tttaccttcg gcaatgaact gggccaaatt ctgattagtt gcagaaatga tgcgaatgtc
    24361 gacttgtatt gggctactgg caccaatcgg cagaatttca cgcgcctcaa tagcgcgcag
    24421 taatttagcc tgcaacatta atggcatatc acctatttca tcgagaaaca gcgtgcccgt
    24481 attcgccgcc tgaatcaacc ctgttttacc gttggcagaa gcgccagtaa atgcgccttt
    24541 aacataaccg aacagttcgc tttccagaag ctgctccgga atcgcggcac agttgatagc
    24601 aataaagggt ttattccgtc ttccgctcaa cttatggatt gctcgggcga cgacttcttt
    24661 acccgtgccg ctttcaccaa ccaccataac gctggatggg ctgggtgcaa tacggctaat
    24721 gagtcgtttt aattgccgca taacacggca ctcgccaacc aattgttcaa tatgtggttc
    24781 atcaggtgca tttgctacag aaaaactggt atgcgattgg tgaaatgcca ttaaaaataa
    24841 ttgtcggccc tgaatgttat gcaattgacc aatgattaat tcacttttat cgtcccacga
    24901 aacaatatgc tgcatatgtc catgagtaaa attactctca aatgttaatg gtctgaaacg
    24961 gataggtttc ccaataatat tattttgtac aacaccaagt gtttttaagg cagtctgatt
    25021 aacaaactga acccgacttt catcatcaac aactaatacg ccctgatcca tattatcgat
    25081 catggtcgcg aatattttac tgatgttatc tcctggcccc tgatcctcca gaagtttcga
    25141 aacaaaaatg gtggatatat ggcgaacata atcagaaaat tcgcgtaaat tatcactgat
    25201 atgctcttgt tgctcgtggg taacggcaat caaacttatc accccaacac aacgatcctg
    25261 taaaatgaca ggcgtaccca gaaatgcttt ttcgcggcaa ttttctttac tatcgcaacc
    25321 ttcgcaaagg ggatcgaagc gagactgtgt cacaactttt tcagttttcg tttccaggac
    25381 gtggcggagc aggcgtgagt tgccgctcaa ctggcgacca agaaacttcc catacgcgcc
    25441 cgttccggcg acgcgacaca agttttcatc aacgatctca acctcaagct gcaaaacgct
    25501 ggcaagcatt ctggcaaaac gctgaattgt cggttgaatt tgcatcaata ctgactgcgt
    25561 agtagcaagc tccatagctt taccttccag acttacttaa aagtcgatca ttgaagacgt
    25621 tgatggttca cagatcatga tgatattaac tcaggcgaaa ttggctttga taaaaacata
    25681 agatttttat cattttctaa tgaaattatg gaagagatat cacatttcta tatcaatatg
    25741 agaattacgg cggtgagttt atcaaactga agagagatag cctgtccctt tatcttattt
    25801 ctgatactta gcagcaaata aataacgcga taaaaaaagc caaacgtttt cgtattttac
    25861 aaacaaccgg aagctggcat caatttgtga tcaaccccac acattatccg tcaaattagt
    25921 cttttgcagc cccgtggata attctggcac acttattgtt agtcccaggt atagctgtga
    25981 aaacaccaat cactttggca agtcacagtg aaataaacca ctttgcctgt cattccacta
    26041 ccgggacttt atgatgaaaa ctgttaatga gctgattaag gatatcaatt cgctgacctc
    26101 tcaccttcac gagaaagatt ttttgttaac gtgggaacag acgccagatg aactgaaaca
    26161 agtactggac gttgccgcag cattaaaagc actgcgtgct gaaaacatct caaccaaagt
    26221 ctttaatagt ggattaggta tttccgtatt ccgcgacaac tccacccgta cccgcttctc
    26281 ttatgcttcc gcgcttaacc tgctcggcct tgcacaacaa gatctcgacg aaggcaaatc
    26341 acaaatcgct cacggcgaaa ccgtgcgtga aaccgccaat atgatctcct tctgcgccga
    26401 cgctattggt attcgcgacg atatgtatct gggcgcaggc aacgcctata tgcgtgaagt
    26461 tggcgctgca cttgatgacg gttacaagca gggtgtactg ccacagcgtc cggctttagt
    26521 gaacctgcaa tgcgatattg accacccgac tcagtcaatg gctgacctcg cgtggttacg
    26581 tgaacacttt ggttcactgg aaaacctgaa aggtaaaaaa atcgccatga cctgggccta
    26641 ctctccaagc tatggcaaac cgctctctgt accacaaggc atcatcggtc tgatgactcg
    26701 cttcggtatg gatgtcaccc tggcccatcc ggaaggctac gacctgatcc cggatgtggt
    26761 tgaagtggcg aaaaacaatg ctaaagcatc tggcggtagc ttccgtcagg tcaccagcat
    26821 ggaagaagcg ttcaaagatg cagacatcgt ttatccgaag tcatgggcac cttacaaagt
    26881 gatggaacag cgtaccgaat tgctgcgcgc taacgatcac gaaggcttaa aagcactgga
    26941 aaaacagtgt ctggcacaga acgcgcaaca caaagactgg cattgtactg aagagatgat
    27001 ggaactgacc cgtgatggcg aagccctgta catgcactgc ctgccagctg atatcagcgg
    27061 cgtatcctgt aaagaaggtg aagtgactga aggcgtattc gaaaaatacc gtatcgcaac
    27121 ctacaaagaa gccagctgga agccttatat catcgccgcg atgatcctgt cccgtaaata
    27181 cgccaaacca ggtgcactgc tcgagcaact gctgaaagaa gcgcaagaac gcgtgaaata
    27241 acccctcggg ccgatcagct ggtcggccct ctatttccag aggccaaaag gataggatat
    27301 gtccgttttc tcattgaaga ttgatatcgc cgataacaaa tttttcaacg gcgaaacatc
    27361 accgctcttt tcgcaaagcc aggccaaact ggcgcgccag ttccaccaga aaatagctgg
    27421 ttatcgccca acaccgcttt gtgcgctgga cgatctcgca aacctttttg gtgtgaagaa
    27481 aattcttgtc aaagacgaat caaaacgatt cggtctgaac gccttcaaaa tgctcggcgg
    27541 tgcgtacgcc atcgctcaat tattgtgtga aaaatatcat cttgatatcg aaacgctgtc
    27601 atttgagcac ctgaaaaatg ccatcggcga aaaaatgact ttcgcgacca ccaccgacgg
    27661 caaccacggg cgcggtgtgg cgtgggcagc gcagcaactc ggacagaatg cggtgattta
    27721 catgccgaaa ggttctgctc aggaacgcgt tgacgccatt ctgaacctcg gtgccgagtg
    27781 catcgtcacg gatatgaact atgacgatac cgttcgcctg accatgcaac acgcgcagca
    27841 gcacggctgg gaagtggtac aggacacggc atgggaaggt tacaccaaaa tcccaacctg
    27901 gatcatgcaa ggctacgcaa ccctggcaga tgaagccgtc gagcaaatgc gtgaaatggg
    27961 cgtaaccccg acgcacgttc tgctgcaagc cggtgtcgga gcaatggccg gtggtgtgct
    28021 gggttatctg gtcgacgtct atagcccgca aaatctgcac agcattattg ttgaacctga
    28081 caaagctgac tgtatttatc gctccggcgt caaaggcgac atcgtcaacg ttggcggtga
    28141 tatggccacc atcatggcgg gcctggcctg tggcgaacct aacccgctgg gctgggaaat
    28201 cctacgtaac tgcgccaccc aattcatctc ctgccaggac agcgttgccg cattaggtat
    28261 gcgcgtgctg ggtaatccgt acggcaacga cccgcgcatc atctccggtg aatccggcgc
    28321 tgtcggtttg ggcgttctcg cggcggttca ttatcacccg caacgtcaaa gcctgatgga
    28381 aaaactggcg ctgaacaaag atgccgtggt gctggttatc agcactgaag gcgacaccga
    28441 cgtgaagcac taccgcgaag ttgtctggga aggcaaacac gctgtagcac cttaatcacc
    28501 ctattggaac tactccctgc aaaacgggga gtaaaaaatc tggagaaaaa taatggctaa
    28561 gaatattcca ttcaaactga ttcttgaaaa agcaaaagat taccaggcgg acatgactcg
    28621 cttcctgcgc gacatggttg ctattcccag tgaaagctgc gacgaaaaac gcgtagtaca
    28681 tcgtattaaa gaagagatgg aaaaagtcgg cttcgataaa gttgaaatcg acccgatggg
    28741 caacgttctc ggttatatcg gccacggccc gcgtctggtg gcaatggacg ctcatatcga
    28801 taccgtcggc attggcaaca tcaaaaactg ggacttcgat ccgtacgaag gcatggaaac
    28861 tgatgagcta atcggtggtc gcggtacttc cgaccaagaa ggcggcatgg catctatggt
    28921 ttatgccggt aaaatcatta aagacctcgg tctggaagat gaatataccc tgctggttac
    28981 cggtactgtg caggaagaag actgcgacgg tctgtgctgg cagtacatta ttgaacaatc
    29041 cggcattcgc ccggaatttg tggtcagtac cgaaccaacc gactgccaga tataccgtgg
    29101 tcagcgcggt cgtatggaaa ttcgtattga tgttcagggt gttagctgcc acggttctgc
    29161 gccagaacgc ggtgacaacg ccattttcaa aatgggtccg attcttggcg aattacaaga
    29221 actctcccaa cgtctgggtt atgacgaatt cctcggcaaa ggcaccctca ccgtttctga
    29281 aatcttcttc acatccccaa gccgttgcgc tgtagcagat agctgcgccg tctctattga
    29341 ccgccgtctg acctggggcg aaacctggga aggcgcgctg gacgaaatcc gcgccctgcc
    29401 tgcagtacag aaagctaacg cggttgtttc tatgtacaac tacgaccgtc cgtcctggac
    29461 tggcctggtt tacccaaccg aatgctactt cccgacctgg aaagtggaag aagatcactt
    29521 caccgttaaa gcactggtga atgcctacga agggctgttt ggcaaagcgc cggttgttga
    29581 taagtggacc ttctcaacta acggcgtatc tatcatgggc cgtcacggca ttccggtgat
    29641 cggctttggc ccgggtaaag aacctgaagc gcatgcacct aacgaaaaaa cctggaaatc
    29701 tcacctggtg acctgtgccg cgatgtacgc tgcaatcccg ttaagctggc tggcaacaga
    29761 gtaattactc ttcacttatt cccctccggt tcgccggagg gtttttggag tttgctatgc
    29821 gcgtattgat caaaaacggc actgtcgtta acgcagatgg acaagccaaa caggatttgc
    29881 tgattgaaag cgggattgtt cgccagttgg gcaacaatat ttcgccgcag ctcccgtatg
    29941 aagaaattga tgccactggc tgttacgttt tccctggcgg cgtggatgtc catacgcatt
    30001 tcaatattga tgtcggcatc gcgcgcagtt gtgatgattt ttttaccggt acccgcgcag
    30061 ctgcgtgtgg cggtacaaca accattattg accatatggg atttggccca aacggctgtc
    30121 ggttacgcca tcaactggag gtttatcgtg gttatgccgc ccataaagcg gtcatcgatt
    30181 acagctttca cggtgtgatc cagcacatta atcacgcaat cctcgacgaa atcccgatga
    30241 tggtcgagga aggactgagc agttttaaac tctatttaac ctatcaatac aaactcaacg
    30301 atgacgaggt tttgcaggca ttacgccgtc tgcatgaatc cggcgcgctg accaccgtgc
    30361 acccggaaaa tgatgcggct atcgccagca agcgggcgga atttatcgcc gcagggttaa
    30421 ccgcgccgcg ctatcatgcc ttgagtcgcc ctctggaatg cgaagcggaa gccatcgccc
    30481 gcatgattaa cctggcacaa attgccggta acgccccgct ctatatcgtg cacctgtcta
    30541 acggcttagg tctggattat ctgcgtcttg cccgtgcgaa tcaccagcca gtctgggttg
    30601 aaacctgccc acaatatctc ctgttggacg aacgcagtta cgatacagaa gacggcatga
    30661 agttcattct tagcccaccg ctgcgtaacg tacgcgagca ggacaaactg tggtgtggca
    30721 tcagcgatgg tgcgattgac gtggtggcga ccgatcactg caccttctca atggctcaac
    30781 gcctgcaaat ttctaaaggc gatttcagtc gctgcccaaa tggcttaccc ggtgtggaaa
    30841 accgcatgca gttactgttt tccagtggcg tgatgacggg acgtataaca ccggaacgct
    30901 ttgttgaatt aaccagcgca atgcccgcca ggctgtttgg cctgtggccg caaaaaggat
    30961 tattagcgcc cggttccgat ggcgacgtgg tgattatcga cccacgtcag agccaacaaa
    31021 ttcagcatcg ccatctccac gacaacgccg actactcgcc atgggagggt tttacctgtc
    31081 agggcgcgat tgtcagaacc ttatcccgtg gtgaaacgat tttctgtgac ggcaccttta
    31141 caggcaaagc cgggcgaggt cgtttcctgc gacgcaaacc gtttgtccct cccgtgctct
    31201 aaccactgtc agggatctat acacaatcaa gaatgatgag tgaggaaaaa tgagtaagaa
    31261 aattgttctc gccctgggcg ggaatgcgct gggcgatgac ctggccggac aaatgaaagc
    31321 ggtaaaaatt acttctcagg caattgttga tttaattgcc cagggacatg aggtcatcgt
    31381 cactcacggt aacgggccac aggtgggcat gattaaccag gcctttgaag ctgccgcaaa
    31441 aactgaagca cactcgccaa tgctgccgat gtctgtctgt gttgccttaa gccagggtta
    31501 tattggttac gatctacaaa acgccttaag agaagaactg ctttctcggg gcattaataa
    31561 acctgtagca acgctggtta ctcaggtcga agtcgacgct aacgatccgg cattcctcaa
    31621 cccgaccaag ccgatcggtt cgttctttac cgagcaagaa gcagagcaac tgacaaaaca
    31681 gggttacact ctgaaagaag atgctggtcg cggctatcgc cgtgttgttg cctcgccaaa
    31741 accggttgat atcattgaaa aagaaacggt taaagctctg gtagatgcag gccaggtggt
    31801 gatcaccgtt ggcggtggtg gtatccctgt tattcgtgaa ggtaaccatc tgcgtggtgc
    31861 cagcgcggtt atcgataaag actgggccag cgcccgttta gcagaaatga ttgatgccga
    31921 tatgctgatc attctgactg cggtagaaaa agtggccatt aacttcggta aagagaatga
    31981 acagtggctc gatcgcctgt cattaagtga tgctgaacgc tttatcgaag aaggtcattt
    32041 tgcgaaaggt tctatgctgc ctaaagtgga agctgccgcc tcgtttgctc gctctcgcgc
    32101 aggtcgtgaa gcactgatta ccgtattgag caaagcgaaa gaagggattg aaggcaaaac
    32161 cggaacggtg atttgtcagt aattttaagg ccgggtgggt aataccatcc ggccattttc
    32221 atttaagcca cttccagcac ttcttttgtc gctttcacgc cctgatgcat caacatcatt
    32281 aacgcctcaa gtacgccgcc gccaatcgcc cgggctttat cagaaacgct ggtgaaatca
    32341 gccgtttcac cacgaggatc gatatcacca attttaaaac caccgaccac tgccaggccg
    32401 tcgttcaaca agccacgcac catccccgtc aacggtgctt taatttcatg ctcaccaatc
    32461 caggcaatca catcgccctc tttcaccaga tcgcctaatt tcacgttgga tcgcataatg
    32521 cctgcagcag gagcacggat cacccgtcgg gtggtatgcc ccataatatt gccaggaaca
    32581 ccggtattct cctgcgcaca accagagtaa atcacctgac cgagccagtg cccgcgattt
    32641 gtttcaatta ccgcatgaca atccttccct gcagtaaagc ccggcccaag cgcgattgtt
    32701 actggtgcca tatctgcccg cgttcccaaa ttctgtttcg ccagaatagc gtccaccacg
    32761 caaagcggtt tcagttcatc aagcaatgaa caggcgggat ctaccatcac agggatgaat
    32821 ccgcgttcgg taagtttcat cgcttccgca gagctggttg ccaggcgagc agtgacgcct
    32881 tcgaccgtca tttcgccatc gaacacggcc tgggcaaacg ccacggtaca acgaatcact
    32941 gtcggttttt ccacttccag catgatcact ttaaaacccg catgatacag acgtagcgcc
    33001 acaccactgg cgatatcacc cgcaccgcga atcaccacca gctggtgtcc ggaaggatga
    33061 gaaggtttca tcatcagccc acccggcgcg ttatttttca cctgtaatat ttctgccagc
    33121 acgctgatgg cgatctcctg cggcgtttcc gcacctatgt tgtaaccaac gggcgcatgt
    33181 aaacgggcaa tatgttcttc agccacgcct ttctcacgca attgacgcag gaaaagctga
    33241 accttgcggc gacttgccag caaccccaac catgcaatgg gctgttcaat gagtttgtcg
    33301 agggcttcac gatcctgatt attcgtggca atcaggacaa aattatcagg gcgaatatcc
    33361 agtgcttcca ctgctgcacc aaacgactca gcatggagaa gcgtggttga tggagggaat
    33421 agttcaggat tgaggctttc gcgataaata tcggcaacgg caatatcaaa tcctaatagc
    33481 gccgcactct gggctatcgc ccggttgaca tgccccgcgc caatcaacac cagacgtgga
    33541 cgcataccat ggacgctgat aaacactgac atcgcacctc cacaatctga cccgacagca
    33601 tccgcaccgt tacgagccat acgtccatgg aataatcgcg gcttacgttc ctgcaatgcc
    33661 tgaagcgact cttcaatcac cttccgctca accattccgc caccaattgt accgacgata
    33721 gagccatcgg cgcgcactaa catttgtgca gaatggcggg gagtcgagcc tcggctatca
    33781 acaatttgcg ccatagcaaa cggacaattt tgctcttcga gttttgcagc ctctgtgaaa
    33841 atattcatac caacctcaat ggatatcctt tcagtaaccc ggaatacccg ggccgctaat
    33901 tcacaaatct gcgcgcgatt gcaggatgtt cttgtatatc gcccagccag attgcctcta
    33961 cgtcgtgttg ttgcagcggt tgaaggagct cgctttgtgc aatcgcattc tcacattgag
    34021 aaaaacggtt aatgaaccag actcgccgac aaccttgcgg tacgttttta aacgccccct
    34081 gagggtggcg aaccagcgca acgagatcgc tcagttgcaa ggttgcatca ggtgttaacc
    34141 cagtaatatc agcaaactgc gaccagcgat ggacattttc tgtgctcact ttcgcgccca
    34201 aagtatgtcc tcccatcaca gcaatcacgc agcaactgct tttgggtatg caaggttcgt
    34261 gctcatcagg cgcttttaac ggcattccac gcgagccatc tgcttcaatg agaattacgt
    34321 cacactctgg tcgttgcacc agtgcatcaa tcgcttctgg cgtaaatccc tgcacttttc
    34381 cctggttcgc tttccagctg tgaaaacaaa atgaaatggg ggatgtaaga gacgcatgag
    34441 gaagcatggc gggatcacga cagaaaacca cgggccagtg agacgttggc ataaacatat
    34501 gtgttgtcgt cgtgattaac acacgcctgc cgctagcctg gagcagttct gccagccaaa
    34561 aaagcaagct ggttttcccc ccagcaccaa caacagaaat cacagtggga cgtttctgcg
    34621 cacctaaatc aatgaataat gccgatgggt caactatact tttcacgagt ctttatgacc
    34681 tctctgggat aaattatccc caacttaatc catcaggaag taacgcaatt atcaggcgtt
    34741 attagcccct ataaataatg gaaccactat gtcagccatc gactgtataa ttaccgctgc
    34801 cggattatca tcaagaatgg ggcaatggaa aatgatgtta ccctggcaac aggggacaat
    34861 tcttgataca agtatcaaaa atgcgttgca gttttgtagc cgaattattt tagtcaccgg
    34921 ctatcgtggt aatgaactgc acgaacgtta tgcgaaccag agcaatatca ctattatcca
    34981 caacccagat tatgcgcagg gtttactaac atcagtaaag gccgcagtac ccgcagtgca
    35041 aacagaacat tgttttctca cccacggtga catgccaacc ctcaccatcg atatttttag
    35101 aaaaatctgg tcgttacgaa atgatggcgc aatactgcca ctccataatg gcatccccgg
    35161 ccatccgatt ttagtgtcaa aaccatgctt gatgcaggca atccaacgac ccaatgtcac
    35221 caatatgcgt caggcactcc ttatgggagc tcattattcc gtcgagatag aaaatgcaga
    35281 aataatttta gatattgata ctccggacga ctttattaca gcgcaaaaaa ggtatactaa
    35341 aatttagaaa atgtagttaa acgattgcgt tcaaatattt aatctctcca gcactcttta
    35401 tcataatgat aaggaatcac tgattttgag aaaagagaaa aagtgataaa tgggctgaca
    35461 ataataattt tcaggagtga ttttaattct catttggcac ctgtgagttc attcacaaaa
    35521 agcctttaag tttcgagccg cctcacattt ttttatattt cccgccaaac ctggcaagag
    35581 tggtgcgatt gttgctctat ccccctaaac caccggattt ctcaacaccg gtcactcaat
    35641 gatatctgta taagctaagg agagggttat gggggatatt atgcgtccca ttccgtttga
    35701 ggaacttttg acgcgcatat ttgatgaata ccaacaacaa cgctcaatct ttggtattcc
    35761 cgagcaacag ttttactcac ctgtaaaagg taaaactgtt agcgtcttcg gtgaaacctg
    35821 tgccactccc gtcggccctg ccgctggccc gcacacgcag ctcgcgcaaa atattgtcac
    35881 ttcctggctg actggcggac gcttcatcga actaaaaacc gtccaaattc ttgaccgcct
    35941 ggagctggaa aagccctgta tcgatgccga agacgagtgc tttaacaccg aatggtctac
    36001 cgaatttacc ctgcttaaag cctgggatga atacctcaaa gcctggtttg ccctgcacct
    36061 tctcgaagcg atgttccagc cttctgattc cggtaaatcg ttcatcttta atatgagcgt
    36121 cggttacaac ctcgaaggta ttaagcaacc gtcaatgcaa cagttcatcg acaatatgat
    36181 ggacgcatct gaccatccga aattcgcgca atatcgcgat acgctgaata aattactcca
    36241 ggatgacgca tttttagccc gccacggatt gcaggaaaaa cgcgaaagct tgcaagcctt
    36301 acccgctcgc atccccacca gtatggtgca gggcgtcacc ctctccacca tgcacggctg
    36361 tcctccgcat gaaatcgaag ccatttgccg ctacatgctg gaagaaaaag ggctcaacac
    36421 ctttgtgaaa cttaacccga ccttactggg atacgcgcgt gttcgtgaga tcctcgatgt
    36481 ctgcggtttc ggttacatag gcttaaaaga agagtcattt gatcacgacc tcaagctgac
    36541 gcaagcactg gaaatgctgg aacgcctgat ggcactggca aaagaaaaat cactcggctt
    36601 tggcgtaaaa ctgactaaca ctctcggcac catcaacaat aaaggcgcac tgcctggtga
    36661 agagatgtat atgtcaggcc gtgcgctgtt cccgctctcc atcaatgttg cagcagttct
    36721 ctctcgcgcc tttgacggca aactgcccat ttcttattcc ggtggtgcca gtcagctgac
    36781 tatccgcgat atttttgata caggtattcg ccctattact atggcaaccg acctgctgaa
    36841 acctggcggc tatttgcgct taagtgcctg catgcgcgag ctggaaggct ccgacgcctg
    36901 gggacttgac catgttgacg tcgaacgact gaacagactg gcagcagatg cgttaaccat
    36961 ggaatacacc cagaaacact ggaagccaga agagcgtatt gaagtggcag aagacctgcc
    37021 gctgaccgat tgctacgttg ccccctgtgt tactgcctgc gctatcaagc aagatattcc
    37081 ggaatacatc cgtctgcttg gcgaacaccg ctatgccgac gcgctggaac tcatctacca
    37141 acacaacgct ctgcccgcca ttaccggtca tatttgcgat caccagtgcc aatacaactg
    37201 tacccgcctg gattacgaca gtgcgctgaa tatccgcgaa ctgaaaaaag tcgcgctgga
    37261 aaaaggttgg gatgaatata agcaacgctg gcacaaaccc gccggttctg gttcacgcca
    37321 tccggttgcc gtgattggtg caggtccggc gggtctggca gcaggttact tccttgccag
    37381 agcgggccat ccggttacgc tgtttgaacg cgaagccaat gctggcggcg tggtgaaaaa
    37441 tatcattcct cagttccgta ttcctgcaga gttaattcag cacgatatcg attttgttgc
    37501 cgctcacggc gtgaaatttg agtatggctg ctcacccgat ttgaccgttg aacagttaaa
    37561 aaatcagggc ttccactatg ttctgattgc caccggcact gataaaaata gcggtgtgaa
    37621 actggcgggc gacaaccaaa atgtctggaa atcactcccc ttcctgcgtg aatacaacaa
    37681 gggtacagcg ctcaagctgg gcaaacatgt ggtcgttgtc ggggcgggta acaccgcaat
    37741 ggactgcgct cgtgcggcgt tacgcgttcc aggcgtaaaa aaagcaacga tcgtttaccg
    37801 tcgttcactg caagagatgc ccgcatggcg cgaagagtat gaagaagcgt tgcacgacgg
    37861 cgtggagttc cgtttcctga ataatccgga acgtttcgat gctgatggca ccttaacctt
    37921 gcgcgttatg tcgcttggcg aaccggatga gaaaggtcgt cgtcgtccgg ttgaaaccaa
    37981 cgaaacagta acactgcatg tagacagcct gatcaccgcc attggtgaac agcaggatac
    38041 tgaagccctg aatgcgatgg gcgtgccgct ggacaaaaac ggctggccag acgtcgacca
    38101 taatggcgaa actcgtctga ctgacgtctt tatgatcggc gacgtacagc gcggaccatc
    38161 ctccattgtc gctgctgtcg gaaccgcgcg tcgggcgacc gatgccatcc ttagtcggga
    38221 aaatatccgt tcccaccaga acgataaata ctggaacaac gtcaatccgg cggaaatcta
    38281 tcaacgtaaa ggcgatatct ctatcacgct ggtgaacagt gacgatcgtg atgcgtttgt
    38341 cgcgcaggaa gctgctcgct gccttgaatg taactacgtt tgcagcaagt gcgtggatgt
    38401 ctgcccgaac cgcgccaacg tctccattgc ggtcccaggc ttccagaacc gtttccagac
    38461 gctgcacctc gacgcttact gtaacgaatg cggcaactgc gctcagttct gtccgtggaa
    38521 cggtaaacca tacaaagaca aaatcaccgt cttcagcctg tcgcaagact ttgataacag
    38581 cagcaaccca ggcttccttg tggaagattg ccgggtacga gtacgtctga ataaccaaag
    38641 ctgggtgtta aacatcgaca gcgaaggtca gttcaacaac gtaccaccgg agctgaacga
    38701 tatgtgccgc atcatcagcc atgtccacca gcatcatcat tatctgctgg gccgcgtgga
    38761 ggtgtaatca tgttgattct gaagaatgtc accgcagtgc agttacaccc ggcgaaagtg
    38821 caggaaggcg ttgatatcgc tatcgaaaat gatgtgattg tcgctatcgg cgatgccctg
    38881 acgcaacgct accccgatgc cagctacaaa gagatgcatg gtcggattgt gatgccggga
    38941 attgtctgct cgcacaacca tttttactcg gggctttccc gcggaattat ggcaaacatc
    39001 gccccctgcc cggatttcat ctcaacgctg aaaaatctct ggtggcggct cgatcgcgcc
    39061 cttgatgaag agtcgctcta ttacagcggg ctgatttgtt ccctggaagc gattaagagc
    39121 ggatgtacat cggttatcga tcaccatgcc tctccagcgt atatcggcgg gtcgctctcc
    39181 acattgcgcg acgcattttt aaaagttggc ctgcgcgcaa tgacctgttt tgaaactact
    39241 gaccgtaaca acggcatcaa agagttgcag gaaggtgtag aagaaaacat ccgctttgcc
    39301 cgtcagattg atgaggcgaa gaaagcagca accgagccgt atctggtgga agcacatatc
    39361 ggtgctcacg cgccgtttac cgtgccagat gccggtctgg agatgctgcg tgaagccgtg
    39421 aaagccacag gtcgtggttt gcatattcac gctgcggaag acctttacga cgtttcctac
    39481 agtcaccact ggtacggcaa agacctgctg gcacgactag cgcaattcga tctcatcgat
    39541 agcaaaacgc tggtcgctca tgggctgtac ttgtcgaaag atgacatcgc cctactcaat
    39601 cagcgcgatg cgttcctggt gcataacgcc cgttcaaaca tgaacaacca tgtcggctac
    39661 aaccatcacc ttagcgacat ccgcaatctg gcgttgggaa cggacggcat tggttcggac
    39721 atgtttgaag agatgaaatt tgccttcttt aaacatcgcg atgcgggtgg tccgctgtgg
    39781 cctgacagtt ttgccaaagc cctgactaac ggtaacgaac tgatgagccg caactttggc
    39841 gcgaaatttg gacttctgga agccggttac aaagctgatt taaccatttg cgattacaac
    39901 tcaccgacgc cgctgctggc agacaatatc gccgggcata tcgctttcgg tatgggctca
    39961 ggcagcgttc acagcgtgat ggtcaatggt gtgatggtct atgaagaccg tcagtttaac
    40021 ttcgattgcg attccattta tgcacaagcc agaaaagccg ctgccagtat gtggcgtcgg
    40081 atggatgcgc tggcataaat gacagatgcc ctcttcccgc agggaagagg gttaaataag
    40141 gaaagaagat gattgaacaa tttttcaggc ccgactctgt cgaacaggcg ctggaactga
    40201 agcgccgcta ccaggatgaa gccgtctggt tcgccggggg cagcaaactc aatgctacac
    40261 caacccgtac cgataaaaag attgccattt ccttacagga tctggaactg gactgggttg
    40321 actgggataa cggtgcactg cggattggcg caatgtctcg cttgcagcca ctgcgtgatg
    40381 cgcgatttat tcctgcagcg ctgcgtgaag ccctcggttt tgtttactca cgccatgttc
    40441 gtaatcagtc gaccattggt ggtgaaatcg ccgcccgcca ggaagagtcg gtgctgcttc
    40501 ccgtcctgtt ggcactggat gctgaactgg tttttggcaa cggcgaaacg ctgtcaatcg
    40561 aggactacct ggcctgccca tgcgatcgcc tgctaaccga aattatcatt aaagatccgt
    40621 atcgcacctg tgcgacccgc aaaattagcc gttctcaggc aggtttaacc gtcgtgacgg
    40681 cagccgttgc aatgacagac cacgacggta tgcgaattgc gctggatggc gtcgccagta
    40741 aagcactgcg tctgcatgat gtcgaaaaac aaaatctgga aggcaatgca cttgaacagg
    40801 ctgtcgccaa cgccattttc ccgcaggaag atttgcgggg cagcgtggcc tataaacgct
    40861 atatcacggg agttctggta gccgacctgt atgccgactg ccaacaggct ggggaggaag
    40921 ccgtatgatc atccacttta ctttaaatgg cgcgcctcag gagctaaccg ttaatccagg
    40981 cgaaaacgtg caaaagctgt tgtttaacat gggaatgcac tctgtacgca acagtgatga
    41041 tggcttcggg tttgccggtt ctgacgcaat aatctttaac ggtaatatcg ttaacgcgtc
    41101 cttgcttatt gccgcacagt tagagaaggc agatattcgt accgcagaat ctttgggcaa
    41161 atggaacgag ttaagtctgg ttcaacaggc aatggttgat gttggcgtgg tgcagtctgg
    41221 ttataacgat ccagctgcag ctctgattat caccgatctt ctcgatcgca tcgccgcacc
    41281 tacccgcgaa gagatcgacg acgcgctttc tggtttgttc agccgcgatg caggctggca
    41341 acaatactat caggtcattg aactggcggt tgcacgtaaa aataatccgc aggccaccat
    41401 tgatatcgct ccgactttcc gtgacgacct tgaagtcatt ggcaagcatt atcctaaaac
    41461 tgatgccgcg aaaatggtgc aggcaaaacc ctgctatgtt gaagaccgcg taacggctga
    41521 cgcctgcgtc attaaaatgt tacgtagccc acacgctcac gcactgatta ctcatctgga
    41581 tgtcagcaaa gctgaagcct taccgggcgt cgttcacgtt attactcacc tgaattgccc
    41641 ggatatctac tataccccgg gtggtcagag cgcaccggaa ccgtcaccgc ttgaccgccg
    41701 tatgttcggc aagaaaatgc gtcacgtcgg cgatcgtgtt gctgcggtcg tcgcagaaag
    41761 tgaagaaatt gcgctcgaag cattgaagct catcgacgtt gaatatgaag tgcttaagcc
    41821 ggtaatgtcg atcgacgaag caatggcgga agatgcgcct gtcgtgcacg atgaaccggt
    41881 ggtgtatgtt gctggtgcgc cagatactct ggaagacgat aacagccatg cagcccagcg
    41941 cggcgagcat atgatcatca acttcccgat cggttctcgc cctcgcaaaa atatcgccgc
    42001 cagtattcat ggtcatattg gcgatatgga caaaggcttt gccgatgccg atgtgatcat
    42061 tgagcgaacc tataactcaa cgcaggcaca gcagtgcccg actgaaacac atatctgctt
    42121 tacccggatg gacggcgatc gtctggttat ccacgcctcc acccaggtac catggcactt
    42181 acgccgccag gtcgcgcgcc tcgtaggcat gaaacagcat aaagttcatg tcattaaaga
    42241 gcgagttggc ggcggttttg gctccaaaca ggacatcctg ctggaagaag tgtgcgcatg
    42301 ggcaacctgc gtgaccgggc gtccggtact gttccgctac acccgtgaag aagagtttat
    42361 tgctaacacc tctcgtcacg tcgcgaaagt caccgtcaaa ctgggagcga aaaaagatgg
    42421 tcgcctgacg gcagtgaaga tggatttccg cgccaacact ggcccttacg gcaaccactc
    42481 actcaccgta ccgtgtaacg gaccggcgct gtcgctgccg ttatatccgt gcgataacgt
    42541 cgatttccag gtcaccacct actacagcaa catttgccca aatggtgctt atcagggtta
    42601 tggcgcaccg aaaggtaact tcgctatcac catggcatta gcggaactgg ctgaacagtt
    42661 acagatcgac caactggaaa ttatcgaacg taaccgggta cacgaagggc aagagctgaa
    42721 aattctcggt gcaatcggtg aaggtaaagc gccgacctcc gttccttccg ccgccagctg
    42781 cgcactggaa gagatcctgc gtcagggtcg cgagatgatc caatggtctt caccgaaacc
    42841 gcaaaatggt gactggcaca tcggtcgcgg cgtcgccatt atcatgcaga aatcgggtat
    42901 cccggatatc gatcaggcta actgcatgat caaactggaa tcggacggca cctttatcgt
    42961 tcattctggc ggtgcggata ttggtactgg tctggatact gtagtgacga aactggcagc
    43021 agaagtgctg cactgcccac cgcaggacgt gcatgttatc tccggtgata ccgatcatgc
    43081 gttgtttgat aaaggcgcat atgcctcgtc cggtacttgc ttctcgggta acgcggcgcg
    43141 tttggcagcg gaaaatctgc gggagaaaat tctgttccac ggcgcgcaaa tgttgggtga
    43201 gccagtggca gatgttcaac tagcaacgcc gggcgtcgtg cgcggcaaga aaggcgaagt
    43261 tagtttcggg gatattgccc ataaaggcga aaccggcacc ggctttggtt cactggtggg
    43321 aactggcagt tatatcacgc ctgatttcgc tttcccgtat ggcgcaaact tcgctgaagt
    43381 tgccgtcaac acgcgtacgg gtgaaatccg cctggataaa ttctacgcct tgctggactg
    43441 cggtacaccg gtcaatccag agttagcgtt gggacaaatc tacggtgcca ccctgcgtgc
    43501 tatcggccac agtatgagcg aagagatcat ttataacgcc gaaggtcacc cgttaacgcg
    43561 tgatttacgc agttacggcg caccgaaaat tgg
//
LOCUS       DAEVZA010000026        41351 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEVZA010000026 DAEVZA010000000
VERSION     DAEVZA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.026"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..324
                     /locus_tag="LAF92_001470"
     CDS             <1..324
                     /locus_tag="LAF92_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBI9790498.1"
                     /translation="YAATVRSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRV
                     AQIPENVPMNLRKIISKAIHRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLA
                     RGRAD"
     gene            complement(321..1277)
                     /locus_tag="LAF92_001471"
     CDS             complement(321..1277)
                     /locus_tag="LAF92_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBI9790499.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            1428..2543
                     /gene="macA"
                     /locus_tag="LAF92_001472"
     CDS             1428..2543
                     /gene="macA"
                     /locus_tag="LAF92_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBI9790500.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            2540..4486
                     /gene="macB"
                     /locus_tag="LAF92_001473"
     CDS             2540..4486
                     /gene="macB"
                     /locus_tag="LAF92_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBI9790501.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(4559..4783)
                     /gene="cspD"
                     /locus_tag="LAF92_001474"
     CDS             complement(4559..4783)
                     /gene="cspD"
                     /locus_tag="LAF92_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBI9790502.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            5106..5426
                     /gene="clpS"
                     /locus_tag="LAF92_001475"
     CDS             5106..5426
                     /gene="clpS"
                     /locus_tag="LAF92_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBI9790503.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            5457..7733
                     /gene="clpA"
                     /locus_tag="LAF92_001476"
     CDS             5457..7733
                     /gene="clpA"
                     /locus_tag="LAF92_001476"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBI9790504.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPMLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(8077..8164)
                     /locus_tag="LAF92_001477"
     tRNA            complement(8077..8164)
                     /locus_tag="LAF92_001477"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8128..8130),aa:Ser,seq:gga)
     gene            complement(8418..8636)
                     /gene="infA"
                     /locus_tag="LAF92_001478"
     CDS             complement(8418..8636)
                     /gene="infA"
                     /locus_tag="LAF92_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBI9790505.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(8920..9624)
                     /gene="aat"
                     /locus_tag="LAF92_001479"
     CDS             complement(8920..9624)
                     /gene="aat"
                     /locus_tag="LAF92_001479"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBI9790506.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(9666..11387)
                     /gene="cydC"
                     /locus_tag="LAF92_001480"
     CDS             complement(9666..11387)
                     /gene="cydC"
                     /locus_tag="LAF92_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBI9790507.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(11388..13154)
                     /gene="cydD"
                     /locus_tag="LAF92_001481"
     CDS             complement(11388..13154)
                     /gene="cydD"
                     /locus_tag="LAF92_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBI9790508.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(13277..14242)
                     /gene="trxB"
                     /locus_tag="LAF92_001482"
     CDS             complement(13277..14242)
                     /gene="trxB"
                     /locus_tag="LAF92_001482"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBI9790509.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            14787..15281
                     /gene="lrp"
                     /locus_tag="LAF92_001483"
     CDS             14787..15281
                     /gene="lrp"
                     /locus_tag="LAF92_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBI9790510.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            15416..19483
                     /gene="ftsK"
                     /locus_tag="LAF92_001484"
     CDS             15416..19483
                     /gene="ftsK"
                     /locus_tag="LAF92_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBI9790511.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPPQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            19638..20249
                     /gene="lolA"
                     /locus_tag="LAF92_001485"
     CDS             19638..20249
                     /gene="lolA"
                     /locus_tag="LAF92_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBI9790512.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            20260..21603
                     /gene="rarA"
                     /locus_tag="LAF92_001486"
                     /gene_synonym="mgsA"
     CDS             20260..21603
                     /gene="rarA"
                     /locus_tag="LAF92_001486"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBI9790513.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPHA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARELPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            21694..22986
                     /gene="serS"
                     /locus_tag="LAF92_001487"
     CDS             21694..22986
                     /gene="serS"
                     /locus_tag="LAF92_001487"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBI9790514.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            23225..25669
                     /gene="dmsA"
                     /locus_tag="LAF92_001488"
     CDS             23225..25669
                     /gene="dmsA"
                     /locus_tag="LAF92_001488"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBI9790515.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGIPAEKIIQLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            25680..26297
                     /gene="dmsB"
                     /locus_tag="LAF92_001489"
     CDS             25680..26297
                     /gene="dmsB"
                     /locus_tag="LAF92_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBI9790516.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            26299..27162
                     /gene="dmsC"
                     /locus_tag="LAF92_001490"
     CDS             26299..27162
                     /gene="dmsC"
                     /locus_tag="LAF92_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HBI9790517.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(27198..27824)
                     /locus_tag="LAF92_001491"
     CDS             complement(27198..27824)
                     /locus_tag="LAF92_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI9790518.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKKK"
     gene            28139..29287
                     /locus_tag="LAF92_001492"
     CDS             28139..29287
                     /locus_tag="LAF92_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9790519.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            29497..30927
                     /locus_tag="LAF92_001493"
     CDS             29497..30927
                     /locus_tag="LAF92_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI9790520.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(30928..31836)
                     /locus_tag="LAF92_001494"
     CDS             complement(30928..31836)
                     /locus_tag="LAF92_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9790521.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            31936..32526
                     /locus_tag="LAF92_001495"
     CDS             31936..32526
                     /locus_tag="LAF92_001495"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HBI9790522.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(32608..33348)
                     /gene="pflA"
                     /locus_tag="LAF92_001496"
     CDS             complement(32608..33348)
                     /gene="pflA"
                     /locus_tag="LAF92_001496"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBI9790523.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(33540..35822)
                     /gene="pflB"
                     /locus_tag="LAF92_001497"
     CDS             complement(33540..35822)
                     /gene="pflB"
                     /locus_tag="LAF92_001497"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBI9790524.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(35877..36734)
                     /gene="focA"
                     /locus_tag="LAF92_001498"
     CDS             complement(35877..36734)
                     /gene="focA"
                     /locus_tag="LAF92_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBI9790525.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(37140..38900)
                     /locus_tag="LAF92_001499"
     CDS             complement(37140..38900)
                     /locus_tag="LAF92_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBI9790526.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            39030..39722
                     /locus_tag="LAF92_001500"
     CDS             39030..39722
                     /locus_tag="LAF92_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBI9790527.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            39921..41009
                     /gene="serC"
                     /locus_tag="LAF92_001501"
     CDS             39921..41009
                     /gene="serC"
                     /locus_tag="LAF92_001501"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBI9790528.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            41080..>41351
                     /locus_tag="LAF92_001502"
     CDS             41080..>41351
                     /locus_tag="LAF92_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBI9790529.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALEL"
BASE COUNT        10291 a        10039 c        10895 g        10126 t
ORIGIN      
        1 tatgccgcta cggtacgcag cctgttgaat aacgatcggg aagccgaacg agaacattta
       61 acggcgttac cggcgctgga tatggaacat tttatgtatc gccagggatt ttccgatgtg
      121 ttccaccgcg tggcgcaaat cccggaaaat gtaccgatga atctgcgcaa aattatctcg
      181 aaagcgatcc atcgctcttc caaacccgat cttgccattg aagtggcaat ggaggccgga
      241 cgtcgtggtg tggactccgt accgacgctg ctgaaaaaaa tgttctcacg cgtgctgtgg
      301 ctggcgcgcg gtcgcgcgga ttaaccgcga aacatcgtgg ccatttgtgg ctgaatagcg
      361 tcgagcatct catagcgccg acggtattca gcccgttttt tactggcgat ttcggcaatc
      421 tcttttcgtg ctatctgtgc tggaaggcgg taatggcgtt cagcatcaca tacgccgcca
      481 accgattccc agaaagcgtt gtaatcggca tggatcttgc cttctttatc acgataacgc
      541 aggctgcggt aaatatgcgt ttcattgctg acggcaataa tctgctctac ctgcaaacgt
      601 tgggcaaaca gacaggccgc ttccatcacg aggcgtttgg gaaatagccc gtggcaggct
      661 ttcgtcgcat tctggatttc ctgatgtgga atttcccatt ttgcgccttg cagtccgcca
      721 ataaacatcg ttcttttccc ctgatattca cacagggtaa acgtgatctc tgccagagga
      781 ataccttcgc tgttgcgaaa caggattgtg ctgtcacctt ctttatccat tgagatcatc
      841 atggtcagct caagcgtgaa ctgctcgccg tttttgcctt ccagcttcgc cagttgcagc
      901 ccgggggtat tcaaatataa gctgaattct tccgccgaca tacatccgcg gagtaacgca
      961 taatggtaac gtaacgcctc cagcaattgc ttacggctaa gattcgccgc aaggtaaggg
     1021 cgatgcagac gcacaggcag tcgcggttgg cgcgttaaca acacattgag attaggccag
     1081 tgggaaagtt cgttcatcca ctcaacgctt aaacgcggca taatcaacga gcgcagcaaa
     1141 aatttctggc gaaaactacg gcgatgccag aatttacccg gccgacactg tccacgtgcc
     1201 agactaagaa aaagtgacag gctgctgaga gattcagatg gcgtaaaggt ccgttcagtt
     1261 agctgcgaca tattcatgaa atcaatggtt atacatgacg tcgatttcac cattgcgtat
     1321 cttaaccaaa catcaatagt gtgattacta acgtaaattt tagggttttg ttgatatttc
     1381 gttgaagtta atgacccgga ttggcatatg gagtattcag aatatttatg aaaaagcgga
     1441 aaaccgtgaa gaagcgttac gttattgcgc tggtgatagt catcgccgga ctgattacgt
     1501 tatggagaat tcttaacgca cccgtgccga cttatcagac actgattgtg cgccccggtg
     1561 atttacagca aagcgtgctg gcgaccggaa agctggacgc gctgcgtaag gttgacgtgg
     1621 gcgcgcaggt cagcggtcag ttgaaaactc tgtcggtggc gattggcgat aaagtaaaaa
     1681 aagaccagct tttaggggtt attgatcctg aacaggctga aaaccagatt aaggaggtcg
     1741 aagcaacgct gatggagcta cgtgcgcagc ggcagcaggc ggaagcggag ctgaaactgg
     1801 cgcgggtgac gtattcccgt cagcaacgtc tggcacaaac gcaggcggtt tcacagcagg
     1861 atctcgacac cgccgcgacg gagatggctg tgaaacaggc gcaaattggc accattgacg
     1921 cgcaaatcaa gcgcaatcag gcttctctcg atacggctaa aaccaatctc gattacactc
     1981 gcatcgttgc cccgatggcc ggggaagtca cgcaaatcac cactctgcaa ggccagacgg
     2041 tgattgccgc acaacaagca ccgaacattc tgacgctggc agatatgagc accatgctgg
     2101 taaaagcgca ggtttctgaa gcggatgtaa tccacctgaa gccggggcaa aaagcctggt
     2161 ttacggtact tggcgatcca ctgacgcgct acgaggggca aatcaaggat gtactaccga
     2221 cgccagaaaa ggttaacgac gctattttct attacgcccg ttttgaagtc cccaacccca
     2281 atggtttgct gcggctggat atgactgcgc aagtgcatat tcagctcacc gatgtgaaaa
     2341 atgtgctgac gatccctctg tcggcgttag gcgatccggt tggcgataat cgttataaag
     2401 tcaaattgtt gcgtaatggt gaaacacgcg agcgtgaagt gacgattggc gcacgtaacg
     2461 ataccgatgt tgagattgtc aaagggcttg aagcgggcga tgaagtggtg attggtgagg
     2521 ccaaaccagg agctgcacaa tgacgccttt gctcgaatta aaggatattc gtcgcagcta
     2581 tcctgccggt gatgagcagg ttgaggtgct gaagggcatc accctcgata tttatgccgg
     2641 tgagatggta gcgattgttg gcgcttcagg ttccggtaaa tcgaccctga tgaatattct
     2701 cggctgtctg gataaggcca ccagcggcac ctatcgcgtc gccggtcagg atgttgccac
     2761 actggacgcc gatgcgctgg cgcaactgcg ccgcgagcat ttcggcttta ttttccagcg
     2821 ttaccattta ctttcgcatt taaccgccga gcagaacgtt gaagtacccg ccgtctatgc
     2881 cggtcttgag cggaaacagc ggctgcttcg cgcccagggg ttactgcaac gactggggct
     2941 ggaagaccgt acagagtatt atccggcaca gctttcgggt ggtcagcaac agcgcgtcag
     3001 catcgcgcgg gcattgatga acggcggtca ggtaattctt gccgatgaac caaccggcgc
     3061 actggacagc cattctggcg aagaggtgat ggcgatcctg catcagctgc gcgatcgcgg
     3121 gcatacggtg attatcgtca cccacgatcc gcaggtcgct gctcaggccg agcgggtgat
     3181 cgaaattcgc gacggcgaaa ttgtgcgcaa tcctcccgcc attgaaaaag tgaatgttgc
     3241 tggcgggacg gagcctgttg tcaacacggt gtctggctgg cggcagtttg tcagcggttt
     3301 taacgaggcg ctgacgatgg catggcgggc gctggcagcg aataaaatgc gtactttact
     3361 gaccatgctg gggattatta tcggtattgc gtcggtggtt tccattgtcg tggtgggtga
     3421 cgccgccaaa caaatggtgc tggcggatat tcgttctatt ggtacgaata ctattgatgt
     3481 ctatcccggg aaagattttg gcgatgacga tccgcaatat cagcaggcgc tgaagtacga
     3541 cgacttaatc gccatccaaa aacaaccgtg ggtcgcctca gccacacctg ccgtctcgca
     3601 aaacctgcgc ctgcgttata acaatgttga tgttgctgcc agtgccaatg gcgtgagcgg
     3661 cgattatttt aatgtctatg gcatgacctt cagtgaagga aacaccttta atcaggagca
     3721 gctgaacggt cgtgcgcagg tcgtggttct cgacagtaat actcgccgcc agcttttccc
     3781 ccataaagca gatgtggttg gcgaggtgat tctggtcggc aatatgcccg ccagagtcat
     3841 tggtgtggcg gaagaaaaac agtcgatgtt tggtagcagt aaagtgctgc gtgtctggct
     3901 accttacagc acgatgtccg ggcgagttat gggccagtcg tggctgaact ccattactgt
     3961 cagggtaaaa gaaggatttg acagcgccga ggcggaacag caactcacgc gtttactttc
     4021 actgcgccac ggaaagaagg atttctttac ctggaacatg gacggcgtct tgaaaactgt
     4081 tgaaaagacc acacgtactt tacaactgtt tctgacgctg gtggcggtga tttcgctggt
     4141 ggtgggcggt attggtgtaa tgaatattat gctggtgtca gtgaccgagc ggacgcggga
     4201 aattggcatt cgcatggctg taggtgcgcg agcaagcgat gttttgcaac agttcctgat
     4261 cgaagccgta ctggtttgcc tggtcggtgg cgcgttggga ataactctgt cactgttaat
     4321 tgctttcacc ttgcagcttt tcttacccgg ctgggagatt ggtttttcac cgttggcact
     4381 gctgctggcg tttctctgct cgacggccac cgggatttta tttggatggt tacccgcacg
     4441 aaatgcggca cgactggatc cagtagatgc tctggcacga gagtaatttt tgagataaaa
     4501 atgccagccg atcgggctgg cattttgcct ttaggatgta cacaatgaga cagaagagct
     4561 atgcgactgc cgcttctact tcgacgggca caataacact ggcgtgattg ccttttggcc
     4621 cctggtggac atcaaactga acggattgtc cagcttttag cgttctgtaa ccatccatct
     4681 gaatggtgga ataatgagcg aaaatatctt cgccgccgcc ttcagggcag atgaaaccaa
     4741 accctttggc attgttgaac cacttaacag tacccttttc catgcttcga catccttcgc
     4801 aaatcttata caagtaagat ggaataaacc ggggtcagag agggggctgt tcaaaacctc
     4861 gccaactcta gaaatacaat ttagagaatt agggcgagcc gtcaagcgtt tgacaggggc
     4921 cagggggcag gtatgaatca aaaatttgaa gcagttaacg ctattgacag gaatgtgaca
     4981 gatgtcgctg atgccaacga tagatgatag ttatctatca tgtggagtag attggtcagg
     5041 caaataagct cttgtcagcg gcagggcgtt ctgccgataa ccgtaaccga agatgataac
     5101 tgacaatggg taaaacgaac gactggctgg actttgatca actggcggaa gaaaaagttc
     5161 gcgacgcgct aaaaccgcca tctatgtata aagtgatatt agtcaatgat gattacactc
     5221 cgatggagtt tgttattgac gtgttacaaa aattcttttc ttatgatgta gaacgtgcaa
     5281 cgcaattgat gctcgctgtt cactaccagg ggaaggccat ttgcggagtc tttaccgccg
     5341 aggttgcaga aaccaaagtg gcgatggtga acaagtacgc gagggagaat gagcatccat
     5401 tgctgtgtac gctagaaaaa gcctgaatgc aggcataaaa attgggggag gtgcctatgc
     5461 tcaatcaaga actggaactc agtttaaata tggctttcgc cagagcgcgc gagcaccgtc
     5521 atgagtttat gaccgtcgag cacttgttac tggcgctgct cagtaaccca tctgcccggg
     5581 aggcgctgga agcgtgttct gtggatttgg ttgcgctccg tcaggaactg gaagccttta
     5641 ttgaacaaac cacacccgtt ctgcctgcca gtgaagagga gcgcgacaca cagccgacgc
     5701 tgagttttca gcgtgtactg caacgtgcgg tcttccatgt ccagtcctcc ggtcgcaatg
     5761 aggtaaccgg tgcaaacgtt ctggtcgcta tctttagcga acaggagtcg caggcggcat
     5821 atctgttgcg taaacatgaa gtcagccgtc tcgatgtggt gaactttatc tctcatggca
     5881 cgcgtaaaga cgagccgaca cagtcttctg atcctggcag ccagccaaac agcgaagaac
     5941 aagctggtgg ggaggaacgt atggagaatt tcacgacgaa cctgaatcag cttgcgcgcg
     6001 tgggcggaat cgacccactg attggtcgtg agaaggagct ggagcgtgct attcaggttc
     6061 tctgccgtcg ccgtaaaaac aacccgctgc tggtggggga atctggtgtc ggtaaaaccg
     6121 cgattgcaga aggtcttgcc tggcgaattg ttcagggcga tgtgccggaa gtgatggctg
     6181 actgtacgat ttactctctc gatatcggtt ctctgttagc gggcacaaaa tatcgcggcg
     6241 actttgaaaa acgttttaaa gcgttgctca agcagctgga gcaggacact aacagcatcc
     6301 tgtttattga tgagatccac accattatcg gtgcgggggc agcgtctggt ggtcaggtcg
     6361 atgcggctaa cctaatcaaa ccgatgctct ccagcggtaa aattcgcgta attggttcga
     6421 caacctatca ggagttcagc aacattttcg agaaagaccg tgctctggcg cgtcgcttcc
     6481 agaaaattga tattactgaa ccgtcgatcg aagaaactgt tcaaatcatc aatggcctga
     6541 aaccgaagta tgaagcgcac cacgacgtgc gttataccgc aaaagcggtg cgtgcagcgg
     6601 tagagctggc ggtgaaatac attaacgatc gtcatctgcc ggataaagcc attgacgtta
     6661 tcgacgaagc gggcgctcgc gcacgcctga tgccggtaag caaacgcaag aaaaccgtta
     6721 atgtggcgga tattgagtcc gtggtggccc gtattgcgcg cattccagag aagagtgttt
     6781 ctcagagtga ccgcgatacc ctgaaaaacc tcggcgatcg cctgaaaatg ctggtcttcg
     6841 gtcaggataa agccattgag gcgctgactg aagccattaa gatggcgcgt gcaggtttag
     6901 gtcacgaaca taaaccggtt ggttcgttcc tgtttgccgg ccctaccggg gtcgggaaaa
     6961 cagaggtgac ggtacagctt tcgaaagcgt tgggcattga gctgctgcgc tttgatatgt
     7021 ccgagtatat ggaacgccat accgtcagcc gtctgattgg tgcgcctccg ggatacgttg
     7081 gttttgatca gggaggtttg ctgactgatg cggtcatcaa gcatccacat gcggtgctgt
     7141 tgctggacga aatcgagaaa gcgcatccgg acgtgttcaa tattctgttg caggtgatgg
     7201 acaacggtac gctgaccgat aacaacggac gcaaagcgga cttccgtaac gtggtgctgg
     7261 tgatgaccac caacgccggg gtacgtgaaa ctgagcgtaa atccattggt cttatccacc
     7321 aggataacag caccgatgcg atggaggaga tcaagaagat ctttacaccg gaattccgta
     7381 accgtctcga caacattatc tggtttgatc atctgtcaac cgacgtgatc catcaggtgg
     7441 tggataaatt catcgtcgag ttgcaggttc agctggatca gaaaggtgtt tctctggaag
     7501 tgagccagga agcgcgtaac tggctggccg agaaaggtta cgaccgggca atgggcgcac
     7561 gtccgatggc gcgtgtcatc caggacaacc tgaaaaaacc gctcgccaac gaactgctgt
     7621 ttggttcgct ggtggacggc ggtcaggtca ccgtcgcgct ggataaagag aaaaatgagc
     7681 tgacttacgg attccagagt gcacaaaagc acaaggcgga agcagcgcat taatctgatt
     7741 gttaggtagg ttggtcaagt ccgtaatctc gaaagaggtt acggactttt tgtttatggg
     7801 gtggaggagg ttcagaccct ttttttaatg atgatggtaa gttgttgata attagtgctg
     7861 cgggaaggta aggataaaaa agggtgctgc aggagaatgg gatggttttg ctttattaac
     7921 aacgggctaa acgtgtagta tttgagttca ctgccgtaca ggcagcttag aaatcgacgg
     7981 ggtgcggtaa aacctttgcg aacgcgttca ctgccgtaca gacagataaa atgcgaaaaa
     8041 aaagctcgca ctttcgtacg agctcttctt taaatatggc ggtgaggggg ggattcgaac
     8101 ccccgatacg ttgccgtata cacactttcc aggcgtgctc cttcagccac tcggacacct
     8161 caccaaattg ttttgctacc aaacctcatg ggtggcaacg gggcgctact atagggagtt
     8221 ggagtaaaac ggtcaagaag aattttaatg ataattattg tttgctcata ctgtaaacaa
     8281 gttgtgcagt atatctacat cgagacaagt tacggactta tacttccaaa gtacttcata
     8341 catatcacaa aataaaaagg ccggttaaac cgacctttta ctcgttcttt ctcttcgccc
     8401 atcaggcggt aaaacaatca gcgactacgg aagacaatgc ggcctttgct caggtcgtac
     8461 ggggtcagtt caacagtcac tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt
     8521 ttaccggaga tgtgtgcagt aaccacgtga ccgttttcta actctacgcg gaacatggta
     8581 ttaggcaacg tttcaagaac ggtaccttgc atttcaatat tgtcttcttt ggccatctaa
     8641 tcctctgggg tatcactacc gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat
     8701 aagtaaagat ttgcgcgcta aatcgcaaca aacaggttcg gcacattact ccgaaaacac
     8761 acggctaagc cgcaccaaaa gcgcaacgta taagggagcg gtgagataaa cgatggcgtt
     8821 acctgacgcg aaaaattcct tatcggcagc ggggtaatga gcgtaaccaa ctctgcgacc
     8881 gcaattataa cactctgggg agaaatgtgc cgaaaacatt cattcttgtg gtgaaaacaa
     8941 gcatcgtggt acccagaaat tattcggcaa tcgtccgagg cgcatttgat tgagataatt
     9001 aaggtaatcc cggcggggaa tttcgcaggc accaagcgat gctgtgtgat cgttaaggac
     9061 ctggcagtcg ataagcttac cgccatgacc gataaattcc tcacagaata ccagaagcgc
     9121 cgttttagac gcattttcca tccggctgaa catggactcg ccacaaaata gcgttccctg
     9181 ggccacgccg tacataccgc cgacaagctc atcttcacgc cagacttcaa tggagtgggc
     9241 atggccgagt tcgtgaaggc gatggtaggc ttcgaccacg ccacgcgtga tccaggttcc
     9301 ttcttcgcga tcgctggcac agccttcaat gacctgacca aaagcgtaat tcatcgtgac
     9361 acgatagggc gagcgtttat gaaatcgctt catactacgg ctgatatgca gtgattctgg
     9421 ccatagcacc gcgcggggat cgggcgacca ccagaggatg gggtcgcctg gagaaaacca
     9481 cggaaaaata ccacgctggt aagccattaa caggcgcgca gggctaagat cgcccccaag
     9541 tgccagcagg ccgttaggct cacgtaatgc gccttccggg gaagggaagg ctattgaatg
     9601 gcgagaaagc tgaaccaggc gcatgaccgc aaaactccac gcaagtcgga tcgttcaata
     9661 atagcttaca aaccctgctt gaactggtaa taacgcccct gtctggcaag cagctctgcg
     9721 tgagtacctt gctcaataat ttgcccgttg tccatcacta ttatttgttg gaaacgagag
     9781 agtccgcgaa gtcgatgggt gaccattaac accgttttct cacgcatcat ttctgcaagc
     9841 aattcaagga tctggctttc ggttgtggca tctaagcctt cggtaggttc atccagcaac
     9901 accagtggcg catcatgtaa cagcgcacgg gcgatagcca gacggcgcag ttcaccaccg
     9961 gagagctggc gtccgccttc acctaaccaa ctgttgagac ctgcatcctc gagcagcttc
    10021 tccaggccaa cgcgacgcaa gctctccgcc agagcctcat cactactgcc aggcgaggcg
    10081 agtaacagat tatcacgcag cgtggcgcta aacagatgca ctcgctgagg aacaacgctg
    10141 atggtctgtc gtagagccgc ttcattcagg ctggctatgg ggctatcgtt aagcaaaatc
    10201 tcgccctgtt gcgggtccca agcgcgggtc agcagttgta acagtgttga tttgccgcat
    10261 ccggttcgcc cgagaatcgc aatatgttcc ccggcgttta cctgaagaga aatcccttta
    10321 agtgcctgtt gggattgctc cggataagtg aactgaacat cccgtaatgt cagcgaaacg
    10381 cgatcggcaa cacgagtttg ggtatcaggg aaggtgactt ccggtttttg atccgttaaa
    10441 tcagtgatcc gaacggcaga ggcaatgact tgccccagat gctgaaatgc acccgttact
    10501 ggtgccagtg cttcaaacgc ggctaacgcg cagaagacaa acagggcaat taacgcgccg
    10561 ggttgagcat tgccgccaac gccgccagac gccatccaca gcatcaggat caccgctaac
    10621 gcgccaatga gtagcattat cgcttgcgac aatgcggtca gttcagattg acggcgttgc
    10681 gcttccagcc attgaatttc tgtattctct agttgcgtgc gataacgatc gctggcacca
    10741 aaaatggtca gctcagcttg cccttgcagc caggccgtca gttgttggcg atactgtccg
    10801 cgaagatgag tcagattttg cccggtgctt tttcccgcac gataaaacag cggtggcatc
    10861 aggaaaagcg tcagtaacat aatgccgccc agcgtaaagg cgagggtgaa atcaaggaaa
    10921 cttaacccga ttgtcaccac cataatcacc acaaaagcgc ccaccagcgg cgagataacg
    10981 cgcagataaa gatgatcgag cgtatcaaca tccgccacca cgcgattgag caattcgccc
    11041 tgacgatagc gcgccagtcc ggcaggggag aggggcagca atttgctgaa ggtgtaaatg
    11101 cgcagatgct gcaacacgcg gaaagtcgcg tcgtgactta ccagacgttc aaaataacgc
    11161 ccggcagtac gggtgattgc tgcgccacgc acgcccgcag cgggtagcat atagttgaag
    11221 ctgtacagtc cggcaacccc cgcaaccgct gaggccgaga ggaaccagcc ggaaagtgtc
    11281 aacagaccga tactggcgag cagcgtgaca attgccagca cgataccaag acttaacatc
    11341 catttatgac gtttatacag tgccagatag ggtagcaaag cgcgcattta aatctcctcc
    11401 tgacgatggg ccagtaatgt ggcgaatggg ccaccagcca cacttaattc cgcgtaacgt
    11461 ccttgctcaa taatccggcc atcctgcata acccaaatga catcccagtc agcaagatct
    11521 tctaactggt gggtgaccat taacgttgtc tggcgcagag aggcggcatt cagcgcctcc
    11581 attacgcgct gttcactgtg agcatcaagg ctggcagcgg gttcatccaa cagtaatagc
    11641 gaacagggat ttagtaacgc acgggccacc gccacgcgct gcgcctgccc cacggaaagg
    11701 cgggcagcct ggtcgccaac aggcgtatca acgccttgtg gcaggagcgg tagaaactcg
    11761 ctgacccagg cgttatccag cgctgcttgt aattcttgtt cgctggcatc aggtcgcgcc
    11821 agtagtacgt tatcccgcaa tgttgctgcc ggtaattgtg ggttttgccc aacccaggag
    11881 agatgtttac gccatgattc tggtgataaa tcgcgtaatt ctatcccgtt gattcgtaat
    11941 gatccctgat atgaaagaaa accagaaagc gcgttcagca gtgagctttt acctgaaccg
    12001 ctgcgaccaa ccaacactgc acgttggcct gctggcaaag taaagttcag cggtccggcc
    12061 agcgttttac cttccggcga cgtgataaac agatcctcgg cctcaatggt caccggatcg
    12121 gtcgatgcta attccgcctc accgcgctgc ggatgggcga gcggggtttc cataaacgtt
    12181 ttcagactgt cagctgcacc aacagcctgg gctttagcat gataaaacgt accgagatcg
    12241 cgcaatggct ggaaaaactc tggcgcaagg atcagggcca gaaaacccgc agccagcgtc
    12301 acaccggtat cgtagtgacc aaaatccagc tcgccgagat aggaaaaacc aaagtagacc
    12361 gccaccagag caattgacag cgaggtaaaa aattcgagaa tgccggagga taaaaacgcc
    12421 agccgtagca cttccattgt ccgttggcgg aaatcttccg aagcagaacg aatactttca
    12481 atttcagctt caccacgacc aaaaatacgc aatgtttcca tgccgcgcag gcgatcgagg
    12541 aaatgcccac ttaagcgagc aagagcgaga aagttacgcc ggttagcatc ggcagccccc
    12601 atcccaacca gcgccataaa caacggaatt aacggtgctg tgcccagcag aatgagcgcc
    12661 gcagcccagt tagaggggaa gatcgccacc acaatcagca acggcaccga cactgccagc
    12721 gccatttgcg gcaggtagcg tgcatagtaa tcatgcatat cgtcaatttg ctcgagtacc
    12781 aacgtcgccc agctccccgc aggtttaccc tgaatccacg ctggccctgc ttgttgcaga
    12841 cggtcgagaa cctgacggcg gatggcaaag cggatatgct gcccggcgtg ataacccacc
    12901 cgttcgcgta accagaccac ccatgcgcgc agtacaaagg tcagaaccag taacgtaaag
    12961 ggaagcagca gggcttcacg gggaatattc tccataatca tatgttgcag aattcgcgcc
    13021 atgaaccagg cctgggcaat gatcaatatg ccgcttacaa agcccagcag acgagaaata
    13081 ttcagccaac gttgggagat gacgctttgc tgttttaacc agcgggttaa ctctttttga
    13141 cgggatttat tcattgcacg cttagcaggt gagttatcag aattatttgc agagcaatgt
    13201 tacaacgggg aaaaaataaa ggcgacccat agtcgcatgg tgtcaccttc tttacttttg
    13261 ttactgattt gtaaaattat tttgcgtcag ctaaaccatc gaggtagcgt tccgcatcaa
    13321 gtgctgccat gcagcctgta ccggccgaag taatggcctg acgataaata tgatccatca
    13381 cgtcacccgc ggcaaagacg ccagggatgc tggtctgggt ggcattacca tgaatacccg
    13441 actgcacttt gatgtagccg ttttccagtt ccagctgccc ttcgaaaatg gcagtattcg
    13501 ggctgtgacc gatagcaaca aacagaccag caacgtcgag tgactcgatg ttatcgctgt
    13561 tttgcgtatc gcgcagacga acgccagtga cacccatttg atcgccggtc acttcttcca
    13621 gcgtacggtt ggtgtgcaga atgatgttgc cgttctccac tttatccatc agacgcttaa
    13681 taaggatttt ttccgcgcgg aaaccgtcac ggcggtgaat cagatgcact tccgaagcga
    13741 tgttagacag atacagcgcc tcttcaaccg cggtattgcc gccgccgatg accgcaactt
    13801 tctggttgcg atagaagaaa ccgtcgcagg ttgcacaagc agaaacccca cggcctttaa
    13861 aggcttcttc agagggcagg ccgagatagc gtgcagaagc tccggtggca ataatcagcg
    13921 cgtcgcaagt gtattcgccg ttatcgccat tcagacggaa cggacggttt tgcagatcca
    13981 ccttgttgat atgatcaaaa atgatctcag tttcaaactt ggtggcatgt tcgtgcatgc
    14041 gctccattaa taacggaccg gtcagatcgt ttggatcgcc aggccagttt tccacttccg
    14101 tggtggtggt cagttggccg cctttttcca tgccggtaat cagcacaggt tgcaggttgg
    14161 cgcgcgccgc gtagacagca gcggtgtatc ccgccgggcc tgaacccagg ataagcagtt
    14221 tactgtgttt ggtcgtgccc atgagatccc catagttgtt ggcagacaat gggcaggatt
    14281 gtagggaatt tacagacgta aaaaaagagt atgacgattt tgttaacaat ttgtgcaatc
    14341 ggcagcatcg ataagcaggt caaattctcc cgtcattatc acctctgcta cttaaatttc
    14401 ccgctttata agccgattaa atgatgaata aacgcccctg ttaatgaata tctggcatgt
    14461 tgtactaaaa atcgatgttt tgctttgaca atcccctggt gttttgcgaa aacattcgag
    14521 gaagaaaaaa aacagtattc ttatatgcgc ataaccatgc atgtaaatac catgtttacc
    14581 gtgctagtga aatctacgta tggcgtggac agacgccatt cgtgatgtcg atagctgcca
    14641 caaggcaacg gtcttctcac cgtagaccca ggcattgcgc gccgtgaatc ttcatgattt
    14701 cggtctatcg tgacgggtag cgactctgaa cagtgatgtt tcagggtcag acaggagtag
    14761 ggaaggaata cagagagaca ataataatgg tagatagcaa gaagcgccct ggcaaagatc
    14821 tcgaccgtat cgatcgtaac attcttaatg agttgcaaaa ggatgggcgt atttctaacg
    14881 tcgagctttc taaacgtgtg ggactttccc caacgccgtg ccttgagcgt gtgcgtcggc
    14941 tggaaagaca agggtttatt cagggctata cggcgctgct taacccccat tatctggatg
    15001 catcacttct ggtattcgtt gagattactc tgaatcgtgg cgcaccggat gtgtttgaac
    15061 aattcaatac cgctgtacaa aaacttgaag aaattcagga gtgtcattta gtatctggtg
    15121 atttcgacta cctgttgaaa acacgcgtgc cggatatgtc agcttaccgt aagttgctgg
    15181 gggaaaccct gctgcgtctg cctggcgtca atgacacacg gacatacgtt gttatggaag
    15241 aagtcaagca gagtaatcgt ctggttatta agacgcgcta acacggaaca ggtgcaaaat
    15301 cggcgtattt tgattacact cctgttaatc catacagcaa cagtactggg gtaacctggt
    15361 actgttgtcc gttttagcat cgggcaggaa aagcctgtaa cctggagagc ctttcttgag
    15421 ccaggaatac actgaagaca aagaagtcac attgacaaag ttaagtagcg gccgccgcct
    15481 tctggaggcg ttgctgatcc ttattgtcct gtttgccgtc tggttgatgg ctgccttact
    15541 aagctttaac ccttcggacc ccagctggtc gcaaacggcc tggcatgaac ctatccataa
    15601 tttaggtggg atgcccggtg cgtggttggc agatacgctg ttctttattt ttggcgtgat
    15661 ggcttacacc attcccgtca ttattgtcgg cggttgttgg tttgcctggc gtcatcagtc
    15721 cagcgacgaa tacattgatt attttgccgt ttcgctacgc atcattggcg ttttggcgct
    15781 catccttacc tcctgtggtc tggcggcaat caacgctgac gatatctggt attttgcctc
    15841 cggtggcgtc attggcagct tactaagtac tacgctacaa ccactgctac acagtagcgg
    15901 gggaactatt gcgctgctct gcgtttgggc tgcgggcctg acgctgttca ccggttggtc
    15961 atgggtgacc attgctgaaa aactcggcgg ctggatttta aacattctca ccttcgccag
    16021 taatcgtacc cgtcgcgatg atacctgggt cgatgaagat gaatatgaag atgacgaaga
    16081 gtatgaagat gaaaaccacg gcaaacagca tgaatcacgc cgtgcccgta ttcttcgcgg
    16141 cgcgctagcg cgtcgtaaac ggttggcgga aaaattcatt aatccgatgg ggcggcaaac
    16201 agacgctgcg ttgttctccg gtaagcggat ggatgatgac gaagagatta cctacactgc
    16261 acgcggtgtg gctgccgacc cggacgacgt cctattttcg ggcaatcgtg caacgcagcc
    16321 agaatatgac gaatacgatc cattattaaa cggtgcgcca attaccgaac ctgtcgctgt
    16381 agcagctgct gctaccacgg cgacacaaag ctgggctgcg ccggttgaac ctgtgactca
    16441 gacgccgcct gttgcctctg ttgatgttcc accctcgcaa cctacagtag cctggcagcc
    16501 tgtaccgggt ccacaaacgg gagagccggt tattgctcct gcaccggaag gttacccaca
    16561 gcagtcacaa tatgcgcagc ctgcagtgca atataatgag ccgctgcaac aaccagtaca
    16621 gccgcagcag ccgtattatg cacctgcagc tgaacaacct gcgcaacaac cttactacgc
    16681 tcctgcggcg gaacaacctg ttcagcaacc gtattacgcc cctgcgccag aacaaccggt
    16741 ggcaggtaac gcctggcaag ccgaagagca gcaatccact tttgctccac agtctacata
    16801 ccagactgag caaacttatc agcaaccagc cgctcaggag ccgttgtacc aacagccgca
    16861 atccgttgaa cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga caaaacccgc
    16921 gcgtccgccg ctttactatt ttgaagaagt ggaagagaag cgagcccgtg aacgtgaaca
    16981 acttgcggcc tggtatcaac cgattccaga accggttaaa gaaccagaac cgatcaaatc
    17041 ttcgttgaaa gcgccttctg ttgcagcagt acctccagta gaagccgctg ccgctgtttc
    17101 tccgctggca tctggcgtga aaaaagcgac actggcgacg ggggctgccg caaccgttgc
    17161 cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt ccgcaagtca aagaggggat
    17221 tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca actcgtcgtg aactggcgtc
    17281 ttacggtatt aagctgccct cacagcgtgc ggcggaagag aaagcccgtg aagcccagcg
    17341 caatcagtat gattctggcg atcagtataa cgatgatgaa atcgatgcga tgcagcagga
    17401 tgaactggca cgtcagttcg cccagacaca gcagcaacgc tatggcgaac agtatcaaca
    17461 tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca gaggctgaac tggctcgtca
    17521 gtttgcgcaa actcaacaac aacgttattc cggcgaacaa ccggctgggg cgaatccgtt
    17581 ctcgctggat gattttgaat tttcgccaat gaaagcgttg ctggatgatg gtccacacga
    17641 accgttgttt acgccaattg ttgaacctgt acagcagccg caacagccgg ttgcgccaca
    17701 gcagcaatat cagcagccgc aacagccggt tgcgccgcag ccgcagtatc agcagccaca
    17761 acagcaggtt gcgccgcagc cacaatatca gcagccgcaa caaccggttg cgccacagcc
    17821 gcagtatcag cagccgcaac aaccggttgc gccgcagccg cagtatcagc agccgcaaca
    17881 accggttgcg ccgccgcagc agtatcagca gccacaacag ccagttgcgc cacaaccgca
    17941 ggataccctg cttcatccgc tgttgatgcg taatggcgac agccgtccgt tgcataaacc
    18001 gacgacgccg ctgccttctc tggatttgct gacaccgccg ccgagcgaag tggagccggt
    18061 agataccttt gcgcttgaac aaatggctcg cctggtggaa gcgcgtctgg ctgatttccg
    18121 tattaaagcc gatgtcgtca attactctcc ggggccggtt atcactcgct ttgaacttaa
    18181 cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg tcacgggacc ttgcccgttc
    18241 actttcgacg gtggcggtgc gtgtcgttga agttattcct ggcaaaccct atgtaggtct
    18301 ggagttacca aataaaaaac gacaaaccgt ttatctgcgc gaagttttgg ataacgccaa
    18361 attccgcgat aatccgtcgc cattaaccgt ggtgctgggt aaagatatcg ccggtgagcc
    18421 ggtggttgcc gatctggcga aaatgccgca cttgttggtt gcggggacta ccggttccgg
    18481 taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt tataaagcac agccagaaga
    18541 tgtgcgtttc atcatgatcg acccgaaaat gctggagctt tcggtttatg aaggcattcc
    18601 gcatctgtta acggaagtcg ttactgatat gaaagatgcc gccaacgcgc tgcgctggtg
    18661 tgttaacgag atggagcgtc ggtataaact gatgtctgcg ctgggtgtgc gtaatctggc
    18721 gggttataac gaaaaaattg ctgaagccga tcgcatgatg cgtccgattc cagacccgta
    18781 ctggaagccg ggtgacagta tggatgccca gcatccggtg ctgaaaaaag aaccatacat
    18841 tgtggtgttg gttgacgaat ttgccgacct gatgatgacg gtaggtaaaa aagtggaaga
    18901 gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt atccacctcg tactggcaac
    18961 tcagcgtcca tcggttgatg ttattactgg tctgattaaa gcgaatattc cgacccgtat
    19021 cgcctttacc gtatccagta agattgactc acgtaccatt cttgatcagg ctggcgcgga
    19081 atcactgctg gggatggggg atatgctcta ctctgggccg aactccacgt tgccggtacg
    19141 tgtccatggt gcttttgttc gcgatcagga agttcatgcc gtggtgcagg actggaaagc
    19201 gcgtggtcgc ccacagtatg ttgatggcat cacttccgac agtgaaagcg aaggtggcgc
    19261 gggcggtttc gatggcgctg aagaactgga tccgttgttc gatcaggcgg tgcagtttgt
    19321 cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt cagttccgca ttggttataa
    19381 ccgtgcagcg cgtattatcg aacagatgga agcgcagggg attgtcagcg aacaggggca
    19441 caacggtaat cgtgaagtgc tggccccacc gccgtttgac taattaatgc attgccggat
    19501 aaggcgcggt agcgtcgcat ccggcactct atcaactgaa aattcagtat tttcttcttt
    19561 cctcaagctg attattagcc tggaatagag agtagaggga actcccgatc gggagtgacg
    19621 taatttgagg attaatgatg aaaaaaattg ccatcacctg tgcattactc tcaagcttag
    19681 tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa aagccgcctg gataaagtca
    19741 gcagcttcca cgccagcttc acacaaaaag tgactgacgg tagcggcgcg gcggtgcagg
    19801 aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt caactggcat atgacacaac
    19861 ctgatgaaag cattctggtt tctgacggta aaacactgtg gttctataac ccgttcgttg
    19921 agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa tacgccgttt atgctgattg
    19981 cccgcaacca gtccagcgac tggcagcagt acaatatcaa acagaatggc gatgactttg
    20041 tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt caccattaac gtgggacgtg
    20101 atggcacaat ccatcagttt agcgcggtgg agcaggacga tcagcgcagc agttatcaac
    20161 tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt taccttcacc ccgccgcaag
    20221 gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag tgagcaatct gtcgctcgat
    20281 ttttcggata atacttttca acctctggcc gcgcgtatgc ggccagaaaa tttagcacag
    20341 tatatcggcc agcaacattt gctggctgcg gggaagccgt tgccgcacgc tatcgaagcc
    20401 gggcatttac attctatgat cctctggggg ccaccgggta ccggcaaaac aaccctcgct
    20461 gaagtgattg cccgctatgc gaacgctgat gtggaacgta tttctgccgt cacctctggc
    20521 gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa accgcaatgc aggtcgccgc
    20581 actattcttt ttgttgatga agttcaccgt ttcaacaaaa gccagcagga tgcatttctg
    20641 ccacatattg aagacggcac catcactttt attggcgcaa ccactgaaaa cccgtcgttt
    20701 gagcttaatt cggcactgct ttcccgtgcc cgtgtctatc tattgaaatc cctgagtaca
    20761 gaggatattg agcaagtact aactcaggcg atggaagaca aaacccgtgg ctatggtggt
    20821 caggatattg ttctgccaga tgaaacacga cgcgccattg ctgaactggt gaatggcgac
    20881 gcgcgccggg cgttaaatac gctggaaatg atggcggata tggccgaagt cgatgatagc
    20941 ggtaagcggg tcctgaagcc tgaattactg accgaaatcg caggtgaacg tagcgcccgc
    21001 tttgataaca aaggcgatcg cttttacgat ctgatttccg cactgcataa gtcggtacgt
    21061 ggtagcgcac ccgatgcggc gctctactgg tatgcgcgaa ttattaccgc cggtggcgat
    21121 ccgttatatg tcgcgcgtcg ttgcctggca attgcttccg aagatgtcgg caatgccgac
    21181 ccacgggcga tgcaggtggc aattgcggcc tgggattgct ttactcgcgt tggcccggcg
    21241 gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg cctgcgcgcc aaaaagcaac
    21301 gctgtctaca ctgcgtttaa agccgcgctg gcggatgctc gcgaacttcc ggattatgac
    21361 gtgccggttc atttgcgtaa tgcgccgacg aaattaatga aggaaatggg ctacgggcag
    21421 gaatatcgtt acgctcatga tgaagcaaac gcttatgctg ccggtgaggt ttacttcccg
    21481 ccggaaatag cacaaacacg ctattatttc ccgacaaaca ggggccttga aggcaagatt
    21541 ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata gccccataaa acgctaccgt
    21601 taatgttatc gttgcggtaa tgttgttact gtatccctgt ggtcgcaggc tgtggccaca
    21661 tctcccattt aattcgataa gcacaggata agcatgctcg atcccaatct gctgcgtaat
    21721 gagccagacg cagtcgctga aaaactggca cgccggggct ttaagctgga tgtagataag
    21781 ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca aaacggaaaa cctgcaagcg
    21841 gagcgtaact cccgatcgaa atccattggc caggcgaaag cgcgcgggga agatatcgag
    21901 cctttacgtc tggaagtgaa caaactgggc gaagagctgg atgcagcaaa agccgagctg
    21961 gatgctttac aggctgaaat tcgcgatatc gcgctgacca tccctaacct gcctgcagat
    22021 gaagtgccgg taggtaaaga cgaaaatgac aacgtcgaag tcagccgctg gggcaccccg
    22081 cgtgagtttg actttgaagt tcgtgaccat gtgacgctgg gtgaaatgca ctctggcctc
    22141 gactttgcag ccgcagttaa gctgaccggt tcccgctttg tggtaatgaa agggcagatt
    22201 gctcgcatgc accgcgcact gtcgcagttt atgctggatc tgcataccga acagcatggc
    22261 tacagtgaga actatgttcc gtacctggtt aaccaggaca cgctgtacgg tacgggtcag
    22321 ctgccgaaat ttgctggcga tctgttccat actcgtccgc tggaagaaga agcagacacc
    22381 agtaactatg cgctgatccc aacggcagaa gttccgctga ccaacctggt acgcggtgaa
    22441 atcattgatg aagatgatct gccaattaag atgaccgccc acaccccatg cttccgttct
    22501 gaagccggtt catatggtcg tgacacccgt ggtctgatcc gtatgcacca gttcgacaaa
    22561 gttgaaatgg tgcagatcgt gcgcccggaa gactcaatgg cggcgctgga agagatgact
    22621 ggtcatgcag aaaaagtcct gcaactgctg ggcctaccgt accgtaaaat tatcctttgt
    22681 accggcgaca tgggctttgg cgcttgcaaa acttacgacc tggaagtatg gatcccggca
    22741 cagaacacct accgcgagat ctcttcctgc tccaacgttt gggatttcca ggcacgtcgt
    22801 atgcaggcac gttgtcgcag caagtcggac aagaaaaccc gtctggttca taccctgaac
    22861 ggttctggtc tggctgttgg tcgtacgctg gttgcggtta tggaaaacta tcagcaggct
    22921 gatggtcgta ttgaagtacc agaagttctg cgtccgtata tgaacggact ggaatatatt
    22981 ggctaatacc caatttttct gaatctaaaa agcgcctgcg ggcgcttttt ttgtctccct
    23041 ttgataccga acaataatta ctcctcactt acacgtaata ctactttcga gtgaaaatct
    23101 acctatctct ttgattttca aattattcga tgtatacaag cctatatagc gaactgctat
    23161 agaaataatt acacaatacg gtttgttact ggaatcaatc gtgagcaagc ttgagtgagc
    23221 cattatgaaa acgaaaatcc ctgatgcggt attggctgct gaggtgagtc gccgtggttt
    23281 ggtaaaaacg acagcgatcg gcggcctggc aatggccagc agcgcattaa cattaccttt
    23341 tagtcggatt gcgcacgctg tcgatagcgc cattccaaca aaatcagacg aaaaggttat
    23401 ctggagcgcc tgtacagtta actgtggtag tcgctgcccg ctacgtatgc acgtcgtgga
    23461 cggtgaaatc aaatatgtcg aaacggacaa taccggcgat gacaattacg acggcctgca
    23521 ccaggttcgc gcctgtctgc gtgggcgttc catgcgtcgc cgtgtctaca atccggaccg
    23581 cctgaaatat ccgatgaaac gagtcggggc gcgcggtgaa ggcaaattcg agcgcattag
    23641 ctgggaagaa gcctacgaca tcatcgcgac caatatgcag cgcctgatca aagagtacgg
    23701 caacgagtct atctatctga actatggcac cggtacgctg ggcggcacca tgacccgctc
    23761 ctggccgccg ggaaataccc tggtcgcgcg cctgatgaac tgctgcggcg gttatcttaa
    23821 ccattacggt gactactctt ctgcgcaaat tgcggaaggt ttgaactata cctacggcgg
    23881 ctgggcagat ggtaacagcc cgtcggatat cgaaaacagt aaattggtag tgctgtttgg
    23941 taataaccca ggcgaaacgc gaatgagtgg cggtggggtg acttactatc ttgaacaggc
    24001 acgccagaaa tctaatgccc gcatgatcat catcgatccg cgctataccg acaccggtgc
    24061 cgggcgcgaa gatgagtgga tccctattcg tccaggaaca gatgccgcac tggttaacgg
    24121 tctggcgtat gtcatgatca ccgaaaacct ggtggatcag gcattccttg ataaatattg
    24181 cgttggctac gatgagaaaa ccctgcccgc tagcgcgccg aaaaatggcc actataaagc
    24241 ttatattctg ggtgaagggc cagatggcgt ggctaaaaca ccgcaatggg cctcgcaaat
    24301 caccggtatc ccggcagaga aaatcattca actggcacgg gaaatcggca gtaccaaacc
    24361 ggcgtttatc agccagggat gggggccaca gcgccacgcc aacggcgaaa tcgcgactcg
    24421 cgctatctcg atgctggcga ttctgaccgg taacgtcggt attaacggag gcaacagcgg
    24481 cgcgcgtgaa ggttcataca gcttaccgtt tgtccgtatg ccgaccttgg aaaacccgat
    24541 ccagaccagc atttcgatgt ttatgtggac cgatgccatt gaacgtggcc cggaaatgac
    24601 agcgctgcgt gatggtgtac gcgggaaaga taagctggat gtgccgatca aaatgatctg
    24661 gaactatgcc ggtaactgcc tgattaacca gcattctgaa atcaaccgta cccatgaaat
    24721 ccttcaggat gataagaagt gcgagctgat tgtggttatc gactgccaca tgacctcatc
    24781 ggcgaaatat gctgacatcc tgctgcctga ctgcaccgct tccgaacaga tggactttgc
    24841 actggatgca tcctgcggga atatgtctta cgtgattttc aacgatcagg tgattaaacc
    24901 gcgctttgaa tgtaagacca tctatgaaat gaccagcgaa ctggcaaaac gtcttggcgt
    24961 tgagcaacag tttactgaag gccgtacaca ggaagagtgg atgcggcatc tgtatgccca
    25021 gtcgcgggaa gcgattcctg aactgccaac gtttgaagag ttccgcaagc aggggatctt
    25081 taaaaagcgc gacccacaag ggcatcacgt tgcttataaa gccttccgtg aagatccgca
    25141 ggcaaaccca ctgactacgc catcgggcaa aattgagatt tattcgcagg cgctggctga
    25201 cattgccgct acctgggaat tgccagaagg cgatgtgatc gatccactgc cgatctacac
    25261 gccgggcttt gaaagttatc aggatccgct gaacaaacag tatccgctgc agcttacggg
    25321 tttccactat aaatctcgcg ttcactcaac ttacggcaac gttgatgtgc tgaaagcggc
    25381 ttgccgtcag gaaatgtgga tcaacccgct tgatgcgcaa aaacgcggta tcaacaacgg
    25441 cgataaagtc aggatcttta acgatcgtgg tgaagttcat attgaggcga aagtgacgcc
    25501 acgaatgatg ccgggtgtgg tcgcactggg tgaaggtgcc tggtatgacc cggatgcaaa
    25561 acgtgtcgat aagggcggtt gtattaacgt actgaccact caacgtccgt ctcctctcgc
    25621 taaggggaat ccgtcacata caaaccttgt tcaggttgaa aaggtgtaag gagtaaccga
    25681 tgacaaccca gtatggattt tttattgatt ccagccgttg caccggttgc aaaacctgcg
    25741 agctggcctg taaagactac aaagatttga cgccagaagt cagcttccgc cgcatttatg
    25801 aatatgctgg cggcgactgg caggaagata acggtgtctg gcaccagaac gtgtttgcct
    25861 actatctgtc gatttcatgt aaccactgcg aagatccggc ttgtactaaa gtctgcccga
    25921 gcggtgcgat gcataaacgc gaagatggtt ttgttgtggt cgatgaagat gtatgcattg
    25981 gctgccgcta ctgtcatatg gcttgcccgt atggcgcacc gcaatataac gaaacgaaag
    26041 gccatatgac caaatgcgat ggttgttatg accgtgttgc ggagggtaaa aagccgatct
    26101 gtgttgaatc ttgcccgctg cgggcgctgg atttcggtcc tatcgacgag ctgcgtaaaa
    26161 aacatggcga tctggcggcc gttgcgccgt tgccgcgagc tcactttacc aaaccgaata
    26221 ttgtgatcaa acccaatgcc aatagccgcc cgaccgggga taccactggc tatctggcaa
    26281 acccgaagga ggtgtgagat gggaagtgga tggcatgaat ggccgctgat gatcttcacg
    26341 gtcttcgggc aatgtgtagc aggtggtttt atcgttctgg ctttggcgct gctcaaaggc
    26401 gacctgcgag cagaagccca gcagcgtgtt atcgcctgca tgtttggttt atgggtgctg
    26461 atgggcattg gctttatcgc ctctatgctc catcttggtt caccaatgcg cgcttttaac
    26521 tcgctcaacc gggtaggggc ttcagcactc agtaacgaaa tcgccagcgg ttcgatcttt
    26581 tttgccgtag gcggcatcgg ctggttgctg gcaatgctga aaaagctgtc accggcattg
    26641 cgtacgctgt ggctgatagt gacgatggtt cttggcgtca tctttgtctg gatgatggtg
    26701 cgtgtgtata acagcattga taccgttccg acctggtaca gcatctggac gccgatgggc
    26761 ttcttcctga cgatgtttat gggcggcccg ttactgggtt acctgttgtt gagtctggct
    26821 ggcgtcgatg gctgggcgat gcgtctgctg ccagccattt ctgtactggc actggtggtg
    26881 agtggcgtga tgtcagtgat gcagggcaca gagctggcaa ctattcatag ttctgtgcag
    26941 caggccgcag cgctggtgcc ggactatggt gcgctgatgt cctggcggat cgtgcttttg
    27001 gccgttgccc tgtgcttgtg gattgcacca cagctaaaag gttatcagcc tgcggttccg
    27061 ctactatccg tctcattcat tctgcttctg gcaggggaat taatcggtcg cggcgtattc
    27121 tatggtttgc atatgaccgt ggggatggcc gtcgcaagct aatcagtaac aaccggggtt
    27181 tcggccccgg ttctctttta tttcttcttc gttaacgtgt cataactggt catcaaatta
    27241 cgataatcag gaatatggtt agagaacaac gtcgccagtc cttcaatatc attacgccag
    27301 tcacggtgca gctcgcacgc cacgccgaac catgtcatca attgcgcacc tgcttgcgac
    27361 atgcgatccc atgccgaatg ccgggtaatt tcattaaagg tccctgacgc gtcagtcaca
    27421 acaaagacat caaacccttc ttcaatggct gaaagcgccg ggaatgccac gcaaacttcg
    27481 gttaccacac cggcaataat taactgtttt ttacctgtcg ctttgactgc ttttacaaaa
    27541 tcttcgttat cccaggcgtt aatatttccc gggcgagcaa tgtaaggtgc atcggggaat
    27601 tgtgctttca gttctggaac taatggaccg ttaggaccgg tttcaaaact ggtggtaaga
    27661 atagtaggta agttgaaata cttagccagg tcacccagcg ccagcacgtt atttttaaac
    27721 ttatcgggtt cgatatcccg tacaagggaa agtaaaccag cctgatgatc aacaagcaaa
    27781 acggcagcat catttttatc aagacgaaca tacggtttgg tcatcctctt ctcctttcga
    27841 gagtcagtgg tacgtctgaa atcggtcaga cgtgatgcat tgctctgaaa gcatagacgg
    27901 gaaatatgag tttgctgtga ccatgaaatt tttcgactga acgaagcgta tgaaatttgt
    27961 gttagatcaa taaaaacaat cagatgagat taatcatctc ttcagcatta gcgttatgaa
    28021 tgagtaatat aaatcgataa taccgccgct gcgatatgcg cgtattttca cccattgaca
    28081 atgtttttgg cggtggcatg atgcgcatga aatttgaact tcctcacggt tttaattcat
    28141 gtccacgtat acccggcctg tcatgctttt gctgtctggc ctgcttttgt tgactctggc
    28201 gatcgcggtg ttaaatacac tcgtgccgct ttggctcgcc caggaacaca tgtccacatg
    28261 gcaggtaggc gttgtcagct catcctattt taccggcaac cttgtcggta cattgctgac
    28321 agggtatgtc attaaacgca ttggctttaa ccgcagctat tatctggcct ccttcatttt
    28381 tgccgctggc tgtgccggcc ttggcctgat gattggtttc tggagctggt tggcgtggcg
    28441 ttttgtcgct ggcgtcggct gtgccatgat ttgggtggtt gttgagagcg cgctgatgtg
    28501 cagtgggacg tcacgtaacc gtgggcgttt gcttgctgca tacatgatgg tttattacgt
    28561 gggaacgttt ttaggccagt tactggtcag caaggtctca accgagctga tgagcgtatt
    28621 gccgtgggtt acaggtttga cattggcagg gatcttaccg ctgttgttta cgcgtgtgct
    28681 gaatcagcag gctgaaaacc atgattcgac gtcaattacg gcaatgctaa aactccgtca
    28741 ggcgcggctt ggcgtgaatg gctgcattat ctcaggaatc gttctgggat ctctatatgg
    28801 cctgatgccg ttgtacctca atcacaaagg ggtgagcaat gccagtattg gtttctggat
    28861 ggcggtactg gtcagtgcgg gtatcctcgg acaatggccg attggacgtc tggcggataa
    28921 gtttggtcga ctgctggtgt tgcgtgttca ggtctttgtc gtcattctcg gcagtatcgc
    28981 gatgcttagc caggcggcga tggcccctgc gttattcatc ctcggtgccg ctggctttac
    29041 gctatatccg gtggcgatgg cctgggcttg tgagaaagtt gaacatcatc aactggtggc
    29101 gatgaaccag gccttactgt tgagttatac tgtgggaagt ctgcttggcc cgtcatttac
    29161 cgctatgcta atgcagaatt tctccgataa tttattgttt atcatgatcg ccagcgtatc
    29221 gtttatctat ttgctgatgc tgctgcgcaa cgccggtcat acgccgaaac ccgttgctca
    29281 cgtgtaaatg aattcaagca gagtgtgaac ttactgtttc acactctgct tttttgtttc
    29341 ttctatctga cttgctttat tccaaatttt attcgtttaa aaataaaatg tgcagcaggt
    29401 tataattttg catttcgcga tttccgcact tcttatttgc cgcgcataat ccctcgtttt
    29461 acagatgccc ctttaatttt ggcgaaggat ttgtctatgg ctgggaatgt tcaggaaaaa
    29521 cagttgcgat ggtacaacat tgcgctgatg tcttttatca ctgtctgggg ttttggcaac
    29581 gttgttaata actatgccaa ccaggggctg gtggttgttt tttcatgggt gtttatcttt
    29641 gcactctatt tcacacctta tgcgctaatt gttggtcagt taggctcgac cttcaaagat
    29701 gggaagggcg gggtcagtac ctggattaaa cacacgatgg gacccggact ggcttatctc
    29761 gccgcgtgga cctactgggt ggtgcatatt ccctatctgg cacaaaaacc ccaggcaatt
    29821 ctgattgcgc tcggttgggc gatgaaaggc gacggttcgc taatcaaaga atattcagtc
    29881 gtagcgttac aggggttaac gctggtgctg tttatcttct ttatgtgggt tgcttcacgc
    29941 ggtatgaaat cgctgaaaat cgtcggttct gtggcaggga ttgcgatgtt tgttatgtcg
    30001 ctcctgtatg tggcgatggc ggtaaccgcg cctgcgatta ctgaagtgca tattgcgacc
    30061 acaaacatta cctgggaaac gttcattcct catatcgact ttacctacat taccactatt
    30121 tcaatgctgg ttttcgcggt tggcggagca gagaagattt ctccttacgt taatcaaacg
    30181 cgcaacccag gaaaagaatt tccaaaaggg atgctatgcc tggcggtgat ggttgcggtt
    30241 tgtgccattc tgggctcact ggcgatgggg atgatgtttg actcgcgtaa tatcccggat
    30301 gacttaatga cgaacggtca atattacgcc tttcagaagc tgggcgagta ttacaacatg
    30361 ggtaatactt taatggtgat ttacgccatt gcgaataccc tgggacaagt agctgcgctg
    30421 gtattctcga ttgatgcccc gcttaaagtg ctattaggtg atgctgacag caaatatatt
    30481 ccagccagtt tatgtcgtac caacgcttct ggtacgcccg ttaatggcta ttttctgacc
    30541 ctggtgctgg tggcaattct gattatgctg ccgactctcg gcatcggtga tatgaacaat
    30601 ctctataaat ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta tttatgggta
    30661 tttgttgcat ttattgcagt cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt
    30721 attcgtaata agcctctggc aatgaccgtc gggatttggt gttttgcctt taccgccttt
    30781 gcctgtttga cggggatctt cccgaaaatg gaagccttca ctgcagagtg gaccttccag
    30841 ttggcgctga atgttgcaac gccgtttgtg ctggtaggat taggactgat attcccgctg
    30901 ctggcgcgta aagcgaatag taaataatta ttgtggtcgg tgacgctgac tctggcagat
    30961 ctgacccgtt tttacataat cgagaaatgc gcgcaagccg caggacatat actggcggtt
    31021 tggataatag atctggaagc ctggacgctc tgtgctccag tcttccagta cacattctaa
    31081 acgaccagta tcaaggtact ccttgatctg ttcatagagc agatacccga taccagcacc
    31141 catcaaaacg gcctccagtt cagcatcaac atcatccaga atgatatttc ctgcgacagc
    31201 aatttccagc tcctttgcaa attgccagtg gaaaggtttg ccgctgggat aacgaaaaac
    31261 aacacattga tgattaagca gatcatgagg atggcggggt ttgccatatc ttgcaaagta
    31321 ctctggcgtt gctgctacac ataatttcac tggcgggccg atcgccacgc tgatcatatc
    31381 tttttcgacg atgccactaa gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgac
    31441 aagagagtcg tcagttgtga gctcgacttt aatatcagga tattcacgtg tgaaaccaac
    31501 cagtaaaggc attaaaaata tacgtgcagc tacccgggcg gcattgattt tgagcgttcc
    31561 tgtcggtgtc agacgaaaat cattcatctc atctaacatg atctgaattt cgtcgaaagc
    31621 tgggcgcaat cgttcataga gattagaacc cgcttccgtt aacgatacgc ttcgggttgt
    31681 ccgattgaat aagcgaattt taagacgttg ttccagtgtt ttaatactat ggctaatggc
    31741 ggacgaggat aagcctaatt catcgcccgc tgcacgaaag ctttgattac gggccacggc
    31801 aaagaaagtg gcaaagtcag acatattcat ccgcattagt gaaaatcctt cattaactca
    31861 tcctgaatca tcacgtttac aggattatgc cgcctgcgta aagtgcgctc cagaacttaa
    31921 cgtggaggta aaattatgca gtctgaacgt atttatttgg tatgggccca tcctcgtcat
    31981 gattcattga ccgcacatat tgctgatgcg atccatcagc gggcaatgga gcggaaaata
    32041 caggtgacgg aactcgattt atatcggcgt aatttcaacc cggtgatgac gtcggaagat
    32101 gaaccagact ggaagaatat ggataaacgt tattctccag aggttcatca gctttattca
    32161 gagctgcttg agcatgacac gttagtggtg gtttttcctc tctggtggta cagcttcccg
    32221 gcaatgctaa aaggatatat tgacagagta tggaataatg ggctggctta tggagatggg
    32281 cacaaattac cattcaataa agttcgttgg gtggcgctgg ttggaggaga caaagaatca
    32341 tttgtccaga tgggctggga aaaaaatata agcgattatt taaaaaatat gtgcagttat
    32401 cttggtattg aagatgccga tgtcactttc ttgtgtaata cagtggtatt cgatggggaa
    32461 gaacttcacg cgagctatta tcagtcgtta ttatctcagg tacgggatat ggtagatgca
    32521 ctataagatg tgttaaaaac gctgtagcag aatgaagcgc ggaataaaaa agcggcaact
    32581 caataaagtt gccgctttac ggggaaatta gaacattacc ttatgaccgt actgctcaag
    32641 aatgcctttc acgcgttcca tggtctcttt cttcggtggt ttaacaccgt cgagtttgta
    32701 ctcttcaccc attgccaccc atttgtgttt gcccagctcg tggtagggga gaagctcgat
    32761 tttctcaacg ttgcccatat cacgggtaaa ttcaccgagg cgatgcgctg aatcgtcatc
    32821 gtcagaccag cctgggacaa caacgtagcg gatccacacc ttcacatttt tgttcgccag
    32881 atatttggcg aactccagcg tgcggtgatt ggaaactcca accagatttt ggtggatctc
    32941 gtcgttcatc tgtttgagat cgagcattac caggtcggtt acttccagca gttcatcaat
    33001 caccggatcg taacgacgaa caaaaccgtt ggtgtccaga caggtatgaa tgccttcttt
    33061 tttgcaggcg cggaaccagt cacgaacaaa ctcagcttgt aggattgcct caccgccgga
    33121 tgcggtaacg ccgccgccgg aagcgttcat aaagtggcga taggtcacca cctctttcat
    33181 taaatcttca acggtaactt ctttaccgcc atgcgtatcc caggtgtcgc ggttatgaca
    33241 atacaggcag cgcatcaggc agccctggaa aaaggtgata aagcgaatac ctgggccgtc
    33301 tacggttcca caggattcaa aggagtgaat gcgaccaata actgacattg cggtgtttct
    33361 ccagatgtgg cccatctgag gccgtgttgg tgcgcagctc gaaggctacg tcgagtctgt
    33421 tttggcagtc accttagagt atagatagct gacaaaaaag gctctcgtgc taaaaaaggc
    33481 cccactttcg tggagccttt attgtacgct ttttactgta cgatttcagt caaatctaat
    33541 tacatagatt gagtgaaggt acgagtaata acgtcctgct gctgttcttt agtcagcgag
    33601 ttgaaacgta ctgcgtagcc agatacacgg atggtcagct gcggatattt ttccgggttt
    33661 tccatcgcgt cgagcagcat ttcacggttc atcacgttaa cgttcaggtg ctgaccacct
    33721 tcgatggatg cttcgtggtg gaagtaacca tccatcagac cagccaggtt ggtcttacga
    33781 acttcgtcgt ctttacccag tgcgttcgga acgatagaga aggtgtagga gataccatct
    33841 ttagcgtaag caaacggcag tttagcaacg gaagtcagag acgctacagc acctttctgg
    33901 tcacgaccgt gcatcgggtt agcacccggt ccgaacggcg cgccagcacg acgaccgtct
    33961 ggggtgttac cagttttctt accatacaca acgttagaag tgatggtcag aacagactga
    34021 gtcgggatag cgtcacggta ggtgtgcagt ttctgaattt tcttcatgaa acgttctacc
    34081 aggtcaacag ccaggtcatc tacacgcgga tcattgttac caaactgcgg gtattcgcct
    34141 tcgatttcga agtcgatagc aagaccgtct tcgtcacgaa tcggtttaac tttcgcatat
    34201 ttgattgcag acagggagtc agcagcaacg gacagaccag cgataccaca cgccatggtg
    34261 cggataacgt cacggtcgtg cagcgccatc agagaggctt cgtagctgta cttgtcgtgc
    34321 atgtagtgga tgatgttcag tgcagtgatg tactgtttag ccagccagtc catgaagtga
    34381 tccatgcgct ccatcacttc atcatagttc aggacatcgc ctttgatcgg ttcagacttc
    34441 ggaccaacct gcattttcag tttttcgtca acgccgccgt tgattgcgta cagcatggtt
    34501 ttcgccaggt ttgcacgcgc accgaagaac tgcatttgtt taccaacgat catcgggctt
    34561 acgcagcaag caatagcgta gtcatcgttg ttgaagtccg gacgcatcag gtcatcgttc
    34621 tcatactgca gagaagaggt gtcgatggac actttagcgg cgaatttctt gaagttcagc
    34681 ggcagttttt cagaccacag aatggtcatg ttcggttccg gagacggacc catggtgtac
    34741 agggtgttca ggaaacggaa gctgtttttg gtaaccaggg tacgaccgtc gaggcccata
    34801 ccaccgatag attcggttgc ccagatcggg tcgccagaga acagttcatc gtactccgga
    34861 gtacgcagga agcgaaccat acgcagtttc atgaccaagt ggtcaaccat ttcctgcgct
    34921 tcttgttcgg tgatcttgcc agctttcagg tcacgttcga tgtacacatc caggaaggtg
    34981 gaggtacgac cgaaggacat tgcagcaccg ttctgagact taacagcagc caggtagccg
    35041 aagtaagtcc actggatagc ttcctgagcg ttggtagccg gaccagagat gtcgtagccg
    35101 tatttcgcag ccatttcttt catctgaccc agagcgcggt gctgttcagc gatttcttcg
    35161 cgcagacgga tagtctgttc caggtttacg ccgttttcca gatcagcctg cagagaagtg
    35221 aactgtgcca gtttgtcttt catcaggtag tcgataccgt acagcgcaac gcgacggtag
    35281 tcaccgatga tacggccacg gccatatgca tctggcagac cggtcagaac accagattta
    35341 cggcaacgca ggatgtccgg agtgtaaacg tcgaacacgc cctggttgtg agttttacgg
    35401 tattcagtga agattttttt gatcatcgga tccagttcgc ggttgtacgc tttgcaggaa
    35461 ccttcgatca ttttgatacc accgaacggg ataagagcac gtttcagcgg agcttcagtc
    35521 tgcagaccaa cgattttctc aagctgcttg ttgatgtagc cagcgtcgtg agaggtgatg
    35581 gtggaagcaa cagcggtgtc aaagtcaact ggcgcgtgag tgcggttttc cagtttaacg
    35641 ccttccatta ctttgtccca cagggtggtg gtcgcttcag tagcgccagc caggaaggac
    35701 tcgtcaccct cgtacggagt gtagtttttc tgaatgaagt cacggacgtt tacttcattc
    35761 tgccagtcac ctttggtaaa accttcccag gctgtggcta acttttcatt aagctcggac
    35821 atgtaacacc taccttctta agtggatttt ttatttactg cgtacttcga caaccattaa
    35881 tggtgatcgt tttcacgcag gtaaatgacc cagtatgtca acccaaccaa caaaccaccg
    35941 ccgataatat taccgatcgt aaccggaatc aggttatcag tgatgaagtt catcacggtc
    36001 aggtgagaaa aattttccgg tgcagaaccg acagcggtcc agaattccgg agatgcgaag
    36061 tcgcggatta caatacccat cgggatcata aacatgtttg cgatactgtg ctcaaaaccg
    36121 ctggcaacaa acatcgcgac cggcagcacc ataatgaacg ctttgtccat caggctacgg
    36181 ccagaataac tcatccatac tgccagacat accatcaggt ttgccaggat accaaggcag
    36241 acggcctcaa taaaagtatg gtgcactttg tggtcggcgg tttgtaggac gtttagtccc
    36301 cattgaccat ttgcggtcat atactcgccg gaaagccaca ttaaaagtac aaacagcagt
    36361 gcgccgacca ggttgccaaa atagacattt agccagtttt tcgccaactg accccaggtg
    36421 atgcgcccac tcgccttagc aacaacaatc aacactgtgg aagtaaagag atcggctccg
    36481 cagacaacac aaagaatcag ccccagagag aagcaaatgc cgccaaccag ttttgccatg
    36541 ccgaagggca ttgtgcctgt gccagtggtt gctgtgatat agaagacgaa tgcgattgag
    36601 atgaaaacac cggcggtaat cgccagatag aaagtcttaa gcggatgttt cgttgcttta
    36661 tagacacccg cctcttcggc cactttggcc attgcagcag gaagtaaaag atcaaaaggg
    36721 ttgtcagctt tcacactaac tctctcttta ttaagtcggc gacgagatac taacaaagca
    36781 ttatagatga gaaattgata tagatcatat ctcgcctggc ttataggccc gtaactcgca
    36841 tggtttttat gcaaatacgg agtaaatatt tgattatcca aataaaaata aattttaaaa
    36901 attaacaaat gagttgaatt ttttccgcat cctccgctaa aacagttaat taaaagggag
    36961 catcaggcga ataaagtaac aatatcgatc gtatttatta aatacaaatt accgatattt
    37021 aaccttataa ttacaattat tttattaatg caaatatatg taaagcgggg cattaaaaaa
    37081 acgccccgta atataactca gactaatcat taagcctaca ttgcgtaggc tatttgattt
    37141 tattttgccc agaatgctgc tttggcgcgc tgcagctttt cgtaggcctt caacaacgac
    37201 tgatgtgcag caaacgcgtg cagatcgcta tctaccggtt gcaggccgta aaacgccgct
    37261 tcgccgctca ttgccgcact ggcggcttct acggcatctg cgccgtacat gcgaacaaac
    37321 gcattcagat attgcagcgg ctggcgatct tcttcctgtg ccagtaataa caacgtttgc
    37381 aggcagcgat aatagttggc gcgttccgga ctaaatactg atgagttaaa ctccatcgtc
    37441 cattcggtcc agaccagagc ctgttccaga tcgccaccag ccagcgccag catggctttt
    37501 aattcaccga tacgcagggt gtaccaaccg ttatccgacc cggtcgccag acccaacagc
    37561 tcacgcacgc gggtaaagtc atcaaaacct tcttcatcca gttgctcgat gaggttcagg
    37621 taatcttctt tttcccactc gctgcctggt agcgaaagaa tcgtttcacg taaatggctg
    37681 cccatactgt tattcgcgag ccacagatct tcagccggat aaatatcgga catgccaggc
    37741 acgataatgc ggcaagcgta gacacccaga tgctcgtaat cggcaatata aacttcttta
    37801 tcttcctttt tgaagatagc catcagcgtg gcgaactctt cttccgtggt gccggagaaa
    37861 ttccagtcca caaacggata atcggcatcc tgcttgaaca ggtcccagga gattaaaccg
    37921 ctggaatcga taaagtgcgt ttcgaggttg gtatgttcag cgacttcttc atcatcgaag
    37981 gttggcggag taaacacatc caaatctttc aggccacgac cttgcagcag ctcggtcacg
    38041 gtacgttcca gtgctacgcc aaaatcagga tgcgcaccga aagaggcaaa gcaggtaccg
    38101 ttagcaggat tgaacagtac cacgcaaatc accggatact ggccgccaag cgaaccgtca
    38161 taagcgaaga ttgggaaacc ctccgcttcc agcgtttcga tggcttcaac tactgctggg
    38221 taacgcgcca gcacgtctgc cgggatctct ggcaggctga tgctttcagc aataatgcgg
    38281 tttttcacgt agcgttcgaa aacttcagac aatccctgaa cgcgtgcttc gttgcgggta
    38341 ttacctgcgg acataccgtt ggagacgtac aggttaccaa tgatattcat cggaatataa
    38401 acggtctgat tgtcggactg acgcgtaaac ggcaggccgc aaataccacg atcttcgtta
    38461 ccggattgta ggtcaatcag catgctgccg gtcagttcat tctccggatc ataaaacgcg
    38521 cgcagacggt catcgagtag tccttctggc acatcgtcat tttcggtcag tgggaaccat
    38581 ttttcgttgg gataatgcac gaacggaccg ttggcgatgg tttcgcccag ccagaaatca
    38641 gcaaaaaagt agttggttga gagacgctcg aaatattcac caagtgcaga agccagcgcc
    38701 gcttttttgg ttgcgccttt tccgttggta aaacacagtg cgcactcttt gtcgcgaata
    38761 tgtacagacc agacgttagg cacaggattc agccaggagg cctcttcaat ctgaaagccg
    38821 aggtctgaaa gtttttgctg gaagcgagcg atggaatctt ccagagcggc atctttgccg
    38881 gggataaatg tttgcgtcat gaaaatcact ttagtcgtac ggaaagcgcg caataatacg
    38941 ggttttatct caaaggcgct atcaccgccg ccatgccgat gaactgttga ctatgctttt
    39001 agcggataaa ccacgataag agcataaaaa tgaaggcgtt cgatctccac cgtatggcat
    39061 ttgataaagt gccttttgat ttccttggcg aagttgcact acgtagtctt tatacctttg
    39121 tactggtttt tttgttcctc aaaatgaccg gaagacgcgg tgtgcgacag atgtcgctgt
    39181 ttgaagtgtt aatcattctc acgctgggtt cagcggcggg agatgtggcg ttttatgatg
    39241 atgtgccgat ggtgccggta cttatcgtct ttatcactct ggcgttgtta taccgcctgg
    39301 taatgtggtt gatggcgcac agtgaaaaac tggaagatct actggaaggt aagccggttg
    39361 ttattattga agatggcgag ctggcctggt cgaaactcaa taactccaac atgacggaat
    39421 ttgagttctt tatggagcta cgattgcgtg gcgtggagca gctggggcag gtacgtctgg
    39481 cgattctcga aaccaacggg caaatcagtg tctatttctt tgaagatgac aaggtgaaac
    39541 cgggtttact tattttaccc agtgattgta cgcagcgtta caaagtggtg ccggagtcgg
    39601 cggactatgc ctgtattcgt tgtagtgaaa tcattcatat gaaagcgggg gaaaaacaat
    39661 tatgtccgcg ctgtgcaaat ccagaatgga cgaaggcaag tcgggcaaaa cgggtgacct
    39721 gacagtaaaa acatcggctt tttgctaata atccgagaga ttcttttgtg tgatgcaagc
    39781 cacatttttg ccctcaacgg ttttactcat tgcgatgtgt gtcactgaat gataaaaccg
    39841 atagccacag gaataatgta ttacctgtgg tcgcaatcga ttgaccgcgg gttaatagca
    39901 acgcaacgtg gtgaggggaa atggctcaaa tcttcaattt tagttctggt ccggcaatgc
    39961 taccggcaga ggtgcttaaa caggctcaac aggaactgcg cgactggaac ggtcttggta
    40021 cgtcggtgat ggaagtgagt caccgtggca aagagttcat tcaggttgca gaggaagccg
    40081 agaaggattt tcgcgatctt cttaatgtcc cctccaacta caaggtatta ttctgccatg
    40141 gcggtggtcg cggtcagttt gctgcggtac cgctgaatat tctcggtgat aaaaccaccg
    40201 cagattatgt tgatgccggt tactgggcgg caagtgccat taaagaagcg aaaaaatact
    40261 gcacgcctaa tgtctttgac gccaaagtga ctgttgatgg tctgcgcgcg gttaagccaa
    40321 tgcgtgaatg gcaactctct gataatgctg cttatatgca ttactgcccg aatgaaacca
    40381 tcgacggtat cgccatcgac gaaacgccag acttcggcaa agatgtggtg gtcgccgccg
    40441 acttctcttc aaccattctt tcccgtccga ttgacgtcag ccgttatggc gtgatttacg
    40501 ctggcgcgca gaaaaatatc ggcccggctg gcctgacaat cgtcatcgtt cgtgaagatt
    40561 tgctgggcaa agcgaatatc gcgtgtccgt cgattctgga ttattccatc ctcaacgata
    40621 acggctccat gtttaacacg ccgccgacat ttgcctggta tctatctggt ctggtcttta
    40681 aatggctgaa agcgaacggc ggtgtagctg aaatggataa aatcaatcag caaaaagcag
    40741 aactgctata tggggtgatt gataacagcg atttctaccg caatgacgtg gcgaaagcta
    40801 accgttcgcg gatgaacgtg ccgttccagt tggcggacag tgcgcttgac aaattgttcc
    40861 ttgaagagtc ttttgctgct ggccttcatg cactgaaagg tcaccgtgtg gtcggcggaa
    40921 tgcgcgcttc tatttataac gccatgccgc tggaaggcgt taaagcgctg acagacttca
    40981 tggttgagtt cgaacgccgt cacggttaat gccgaaattt tgcttaatcc ccacagccag
    41041 cctgtggggt ttttatttct gttgtagaga gttgagttca tggaatccct gacgttacaa
    41101 cccatcgctc gtgtcgatgg cactattaat ctgcccggtt ccaagagcgt ttctaaccgc
    41161 gctttattgc tggcggcatt agcacacggc aaaacagtat taaccaatct gctggatagc
    41221 gatgacgtgc gccatatgct gaatgcatta acagcgttag gggtaagcta tacgctttca
    41281 gccgatcgta cgcgttgcga aattatcggt aacggcggtc cattacacgc agaaggtgcc
    41341 ctggagttgt t
//
LOCUS       DAEVZA010000027        41308 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEVZA010000027 DAEVZA010000000
VERSION     DAEVZA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.027"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..161
                     /locus_tag="LAF92_001503"
     CDS             <1..161
                     /locus_tag="LAF92_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBI9790530.1"
                     /translation="LKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLNY
                     TLCEMTKS"
     gene            174..653
                     /gene="eutP"
                     /locus_tag="LAF92_001504"
     CDS             174..653
                     /gene="eutP"
                     /locus_tag="LAF92_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBI9790531.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            628..1329
                     /gene="eutQ"
                     /locus_tag="LAF92_001505"
     CDS             628..1329
                     /gene="eutQ"
                     /locus_tag="LAF92_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBI9790532.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1326..2129
                     /gene="eutT"
                     /locus_tag="LAF92_001506"
     CDS             1326..2129
                     /gene="eutT"
                     /locus_tag="LAF92_001506"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBI9790533.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2126..3142
                     /gene="pta"
                     /locus_tag="LAF92_001507"
     CDS             2126..3142
                     /gene="pta"
                     /locus_tag="LAF92_001507"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBI9790534.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3181..3474
                     /gene="eutM"
                     /locus_tag="LAF92_001508"
     CDS             3181..3474
                     /gene="eutM"
                     /locus_tag="LAF92_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBI9790535.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3581..3868
                     /gene="eutN"
                     /locus_tag="LAF92_001509"
     CDS             3581..3868
                     /gene="eutN"
                     /locus_tag="LAF92_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBI9790536.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            3880..5283
                     /locus_tag="LAF92_001510"
     CDS             3880..5283
                     /locus_tag="LAF92_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBI9790537.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5294..6130
                     /gene="eutJ"
                     /locus_tag="LAF92_001511"
     CDS             5294..6130
                     /gene="eutJ"
                     /locus_tag="LAF92_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBI9790538.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6120..7307
                     /gene="eutG"
                     /locus_tag="LAF92_001512"
     CDS             6120..7307
                     /gene="eutG"
                     /locus_tag="LAF92_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBI9790539.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDHD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7424..8650
                     /gene="eutH"
                     /locus_tag="LAF92_001513"
     CDS             7424..8650
                     /gene="eutH"
                     /locus_tag="LAF92_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBI9790540.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8647..10050
                     /gene="eutA"
                     /locus_tag="LAF92_001514"
     CDS             8647..10050
                     /gene="eutA"
                     /locus_tag="LAF92_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBI9790541.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10062..11423
                     /gene="eutB"
                     /locus_tag="LAF92_001515"
     CDS             10062..11423
                     /gene="eutB"
                     /locus_tag="LAF92_001515"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HBI9790542.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11444..12331
                     /gene="eutC"
                     /locus_tag="LAF92_001516"
     CDS             11444..12331
                     /gene="eutC"
                     /locus_tag="LAF92_001516"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HBI9790543.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCTTTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12341..13000
                     /gene="eutL"
                     /locus_tag="LAF92_001517"
     CDS             12341..13000
                     /gene="eutL"
                     /locus_tag="LAF92_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBI9790544.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13013..13513
                     /gene="eutK"
                     /locus_tag="LAF92_001518"
     CDS             13013..13513
                     /gene="eutK"
                     /locus_tag="LAF92_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBI9790545.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13559..14611
                     /gene="eutR"
                     /locus_tag="LAF92_001519"
                     /pseudo
     CDS             13559..14611
                     /gene="eutR"
                     /locus_tag="LAF92_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(14617..15516)
                     /gene="hemF"
                     /locus_tag="LAF92_001520"
     CDS             complement(14617..15516)
                     /gene="hemF"
                     /locus_tag="LAF92_001520"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBI9790546.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15520..16389)
                     /gene="amiA"
                     /locus_tag="LAF92_001521"
     CDS             complement(15520..16389)
                     /gene="amiA"
                     /locus_tag="LAF92_001521"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBI9790547.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDELLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16603..17028
                     /locus_tag="LAF92_001522"
     CDS             16603..17028
                     /locus_tag="LAF92_001522"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBI9790548.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17015..17464
                     /locus_tag="LAF92_001523"
     CDS             17015..17464
                     /locus_tag="LAF92_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBI9790549.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            17525..18100
                     /locus_tag="LAF92_001524"
     CDS             17525..18100
                     /locus_tag="LAF92_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBI9790550.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18196..19095
                     /gene="yfeX"
                     /locus_tag="LAF92_001525"
     CDS             18196..19095
                     /gene="yfeX"
                     /locus_tag="LAF92_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBI9790551.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19153..20457)
                     /gene="pbp4b"
                     /locus_tag="LAF92_001526"
     CDS             complement(19153..20457)
                     /gene="pbp4b"
                     /locus_tag="LAF92_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HBI9790552.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20462..21886)
                     /gene="murP"
                     /locus_tag="LAF92_001527"
     CDS             complement(20462..21886)
                     /gene="murP"
                     /locus_tag="LAF92_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBI9790553.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(21890..22786)
                     /gene="murQ"
                     /locus_tag="LAF92_001528"
     CDS             complement(21890..22786)
                     /gene="murQ"
                     /locus_tag="LAF92_001528"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBI9790554.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            22950..23807
                     /gene="murR"
                     /locus_tag="LAF92_001529"
     CDS             22950..23807
                     /gene="murR"
                     /locus_tag="LAF92_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBI9790555.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            23936..24727
                     /gene="ucpA"
                     /locus_tag="LAF92_001530"
     CDS             23936..24727
                     /gene="ucpA"
                     /locus_tag="LAF92_001530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBI9790556.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            24885..25901
                     /gene="cysP"
                     /locus_tag="LAF92_001531"
     CDS             24885..25901
                     /gene="cysP"
                     /locus_tag="LAF92_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBI9790557.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            25901..26734
                     /gene="cysT"
                     /locus_tag="LAF92_001532"
     CDS             25901..26734
                     /gene="cysT"
                     /locus_tag="LAF92_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBI9790558.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            26734..27609
                     /gene="cysW"
                     /locus_tag="LAF92_001533"
     CDS             26734..27609
                     /gene="cysW"
                     /locus_tag="LAF92_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBI9790559.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            27599..28696
                     /gene="cysA"
                     /locus_tag="LAF92_001534"
     CDS             27599..28696
                     /gene="cysA"
                     /locus_tag="LAF92_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBI9790560.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            28830..29741
                     /gene="cysM"
                     /locus_tag="LAF92_001535"
     CDS             28830..29741
                     /gene="cysM"
                     /locus_tag="LAF92_001535"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBI9790561.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANHGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(29744..30112)
                     /locus_tag="LAF92_001536"
     CDS             complement(29744..30112)
                     /locus_tag="LAF92_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBI9790562.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30217..31068
                     /gene="pdxK"
                     /locus_tag="LAF92_001537"
                     /gene_synonym="thiJ"
     CDS             30217..31068
                     /gene="pdxK"
                     /locus_tag="LAF92_001537"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBI9790563.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31110..31619)
                     /gene="crr"
                     /locus_tag="LAF92_001538"
     CDS             complement(31110..31619)
                     /gene="crr"
                     /locus_tag="LAF92_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBI9790564.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(31660..33387)
                     /gene="ptsI"
                     /locus_tag="LAF92_001539"
     CDS             complement(31660..33387)
                     /gene="ptsI"
                     /locus_tag="LAF92_001539"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBI9790565.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33432..33689)
                     /gene="ptsH"
                     /locus_tag="LAF92_001540"
     CDS             complement(33432..33689)
                     /gene="ptsH"
                     /locus_tag="LAF92_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBI9790566.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34073..35044)
                     /gene="cysK"
                     /locus_tag="LAF92_001541"
     CDS             complement(34073..35044)
                     /gene="cysK"
                     /locus_tag="LAF92_001541"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBI9790567.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35229..35990)
                     /gene="cysZ"
                     /locus_tag="LAF92_001542"
     CDS             complement(35229..35990)
                     /gene="cysZ"
                     /locus_tag="LAF92_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBI9790568.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36220..37206
                     /gene="zipA"
                     /locus_tag="LAF92_001543"
     CDS             36220..37206
                     /gene="zipA"
                     /locus_tag="LAF92_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBI9790569.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37277..39292
                     /gene="ligA"
                     /locus_tag="LAF92_001544"
     CDS             37277..39292
                     /gene="ligA"
                     /locus_tag="LAF92_001544"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBI9790570.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDCPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39294..39512
                     /locus_tag="LAF92_001545"
     CDS             39294..39512
                     /locus_tag="LAF92_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBI9790571.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39509..40507)
                     /locus_tag="LAF92_001546"
     CDS             complement(39509..40507)
                     /locus_tag="LAF92_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBI9790572.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            40597..>41308
                     /locus_tag="LAF92_001547"
     CDS             40597..>41308
                     /locus_tag="LAF92_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9790573.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGI"
BASE COUNT         9606 a        10613 c        11632 g         9457 t
ORIGIN      
        1 cgctgaaagc cgctgatgtg catatcggtt ttctcgaccg cttcagcggt gcattggtga
       61 tttatggctc ggtgggcgca gtagaagaag cgttatcaca aaccgtcagc ggtttaggtc
      121 gcttattaaa ttatacgctt tgtgaaatga ccaaaagtta atacgaggca ttcatgaaac
      181 gaattgcgtt tgtgggtagc gttggggcag gaaaaacaac gttatttaat gcgttgcagg
      241 ggaattatac cctcgccaga aaaacacagg ccgtggaatt taatgataag ggcgatattg
      301 atacgccggg tgaatatttt agccatcccc gctggtatca cgccttaatt accacgctac
      361 aggatgtaga catgctgatc tatgtccatg gcgcaaatga cccggaaagt cggttgcctg
      421 ccgggttgct ggatattggc gtcagcaaac ggcaaatcgc cgtcatcagt aaaacggaca
      481 tgccagatgc tgacgtcgcc gcaacgcgaa agttattgct tgagacgggt ttcgaagagc
      541 cgattttcga gctaaacagc cacgacccgc aaagcgtgca gcaactggtg gattatctgg
      601 catctctcac caaacaggag gaagcaggtg aaaaaactca tcacagcgaa tgatattcgt
      661 gaagcacacg cgcgcggcga acaggcaatg tccgtggttc tgcgcgccag cattattacc
      721 ccggaagccc gtgaagtggc ggatctactg ggttttacca tcaccgaatg tgatgaatcg
      781 atcccggtaa cggcgtccgt gcccgccagc gtgccagccg acaaaaccga aagccagcgc
      841 atccgcgaaa ccatcattgc ccagctgccg gaaggccagt ttaccgaaag cctggtcgca
      901 cagctgatgg aaaaagtgat gaaggaaaaa cagtcgctgg agcagggcgc aatgcagccg
      961 agcttcaaat cggtgaccgg caaaggcggc atcaaagtga ttgatggcag cagcgtcaag
     1021 tttggtcgtt tcgacggcgc agagccgcat tgcgtgggct tgactgatct tgtgaccgga
     1081 gacgacggca gcagcatggc cgctggtttt atgcagtggg agaacgcatt cttcccgtgg
     1141 accctgaact acgacgaaat cgacatggtg ctggaagggg agctgcatgt tcgccacgaa
     1201 ggcgaaacca tgatcgccaa agcgggtgac gtgatgttta tcccgaaagg ctccagtatc
     1261 gaatttggta cgacatccag cgtgaaattc ctgtatgtcg cctggccggc taactggcaa
     1321 tccctatgaa agatttcatc accgaagcat ggctaagagc gaaccatacg ctcagcgaag
     1381 gcgcagagat ccatctccct gcggacagtc gcctgacgcc ctctgcccgg gagttactgg
     1441 aaagccgcca tctgcgcatc aagtttattg acgagcaggg ccgcctgttt gttgacgatg
     1501 aacagcagca gccgcagccc gttcatgggc tgaccagtag cgatgaacat ccgcaggcgt
     1561 gctgcgaact gtgccgccag ccggtggcaa aaaagccgga tacgctgact cacctgtcgg
     1621 cggaaaaaat ggtcgccaaa agcgatccgc gtctgggttt tcgcgcggtt ctcgacagca
     1681 ccattgcgct ggcagtgtgg ctgcaaattg aactggcgga accgtggcag ccgtggctgg
     1741 cggatatccg ttcgcgtctt ggcaacatta tgcgcgccga tgcgctgggt gaaccgctgg
     1801 gctgccaggc gattgtcggg ctttctgacg aagatctgca tcgactttct caccagccgc
     1861 tgcgctatct cgaccacgat catctggtgc cggaagccag ccacgggcgc gatgcggcgt
     1921 tgctcaatct gctacgtacc aaagtgcggg aaaccgaaac cgtagcggcg caggtgttta
     1981 tcacccgcag ctttgaagta ttacgcccgg acattttgca ggcgctgaac cgcctttcca
     2041 gcacggtcta cgtgatgatg atcctgagcg taacgaagca gccgctaacg gtgaagcaga
     2101 tccaacaacg actgggggaa acgcaatgat tattgaacgt tgtcgtgaac tggcgttgcg
     2161 agcgcccgcc agagtggttt ttccggatgc gttagaccaa cgtgtgctga aagccgcgca
     2221 atatttacat caacaaggtc tggcaacgcc cattctggtc gccaatccgt ttgaacttcg
     2281 tcagtttgcg ctcagtcacg gcgtggcgat ggacgggcta caggtgattg atccgcatgg
     2341 caacctcgca atgcgggaag aatttgctca tcgctggctg gcccgcgcgg gcgaaaaaac
     2401 gccgccggat gcgctggaaa aacttaccga ccctctgatg ttcgccgcag caatggtcag
     2461 cgccggtaaa gcggatgtct gtatcgcggg caacctctct tccacggcaa atgtgctgcg
     2521 tgccggatta cgcattatcg gcttgcagcc aggctgtaaa acgctctctt ccattttcct
     2581 gatgttgcca cagtacagcg gcccggcgtt gggctttgcc gattgcagcg tggtgccaca
     2641 gccgacggcg gcgcagctgg cggatatcgc gcttgccagt gccgaaacct ggcgcgccat
     2701 caccggagaa gagccgcgcg tggcgatgct gtcgttttcc agcaatggta gcgcccgtca
     2761 cccctgtgtt gctaacgtcc agcaggcgac agaaatcgtc cgtgagcgcg caccaaagct
     2821 ggtggtggat ggcgagttac agtttgacgc cgccttcgtg ccggaagtgg cggcgcaaaa
     2881 agcgcctgcc agcccgctac agggcaaggc caatgtgatg gtttttccgt cgctggaagc
     2941 cggaaatatt ggttacaaaa tcgcacaacg actcggcgga tatcgtgccg tcgggccact
     3001 gatacaagga cttgccgcgc cgatgcacga tctctctcgt ggttgtagcg tgcaggaaat
     3061 tatcgagctg gcgctggtgg cagctgtgcc gcgtcagaca gaagtgaacc gcgaaagcag
     3121 tttacaaaca ctggttgaat gaacggtccc gttctggacc ctgataaaga ggaaagcaca
     3181 atggaagcat taggaatgat cgaaacccgg ggcctggttg cactgattga ggcttctgac
     3241 gcgatggtga aagctgcgcg cgtcaagctg gttggtgtta agcagattgg cggcggcctg
     3301 tgtactgcca tggttcgtgg tgatgttgca gcctgtaaag ccgcgaccga tgcaggtgcc
     3361 gcagcggcac agcgtatcgg cgagctggta tctgtacacg ttatcccgcg tccgcacggc
     3421 gacctggaag aagtcttccc gatcggcctg aaaggcgata gcagcaacct gtaatcaccc
     3481 tgtgtgacgt cgattcgggc gtcacgctct ctcctttttc attttacctt ctgcggaggg
     3541 tagggggagc ttttacctga aatacgccac ggaggcgggt atgaaactgg cagtcgtcac
     3601 tggacaaatt gtttgtaccg tacgccatca cggactggcg catgacaaat tgctgatggt
     3661 ggaaatgatt gatccacaag gtaatcctga cgggcaatgc gccgtcgcca tcgacaatat
     3721 tggcgcggga accggggagt gggtgttact ggtgagtggc agttccgccc gccaggcgca
     3781 taaaagcgaa acgtcaccgg tcgatctgtg cgtgattggc attgtcgatg aggtggtgtc
     3841 tggcggtcag gtaattttcc acaaataagg cagaacatca tgaatcaaca ggatattgaa
     3901 caggtggtga aagcggtact gctgaaaatg caaagcagtg acacgccgtc cgccgccgtt
     3961 catgagatgg gcgttttcgc gtccctggat gacgccgttg cggcagccaa agtcgcccag
     4021 caagggttaa aaagcgtggc aatgcgccag ttagccattg ctgccattcg tgaagcaggc
     4081 gaaaaacacg ccagagattt agcggaactt gccgtcagtg aaaccggcat ggggcgcgtt
     4141 gaagataaat ttgcaaaaaa cgtcgctcag gcgcgcggca caccaggcgt tgagtgcctc
     4201 tctccgcaag tgctgactgg cgacaacggc ctgaccctaa ttgaaaacgc accctggggc
     4261 gtggtggctt cggtgacgcc ttccactaac ccggcggcaa ccgtaattaa caacgccatc
     4321 agcctgattg ccgcgggcaa cagcgtcatt tttgccccgc atccggcggc gaaaaaagtc
     4381 tcccagcggg cgatcacgct gctcaaccag gcgattgttg ccgcaggcgg gccggaaaac
     4441 ttactggtta ctgtggcaaa tccggatatc gaaaccgcgc agcgtttgtt caagtttccg
     4501 ggcatcggcc ttctggtggt gaccggcggc gaagcggtag tagaagcggc gcgtaaacac
     4561 accaataaac gtctgattgc cgcaggcgct ggcaacccgc cggtagtggt ggatgaaacc
     4621 gccgacctcg cccgtgccgc tcagtccatc gtcaaaggcg cttctttcga taacaacatc
     4681 atttgtgccg acgaaaaggt actgattgtt gttgatagcg tagccgatga actgatgcgt
     4741 ctgatggaag gccagcacgc ggtgaaactg accgcagaac aggcgcagca gctgcaaccg
     4801 gtgttgctga aaaatatcga cgagcgcgga aaaggcaccg tcagccgtga ctgggttggt
     4861 cgcgatgcgg cgaaaatcgc ggcggcaatc ggcctgaacg tgccgcaaga aacgcgtttg
     4921 ctgtttgtgg aaaccactgc agaacatccg tttgccgtga ccgaactgat gatgccggtg
     4981 ctgcccgtcg tgcgcgtcgc caacgtggcg gatgccattg cgctggcggt gaaactggaa
     5041 ggcggttgcc accacacggc ggcaatgcac tcgcgcaaca tcgaaaacat gaaccagatg
     5101 gcgaatgcta ttgataccag cattttcgtt aagaacggac cgtgcattgc cgggctgggg
     5161 ctgggcgggg aaggctggac caccatgacc atcaccacgc caaccggtga aggggtaacc
     5221 agcgcgcgta cgtttgtccg tctgcgtcgc tgtgtattag tcgatgcgtt tcgcattgtt
     5281 taaggagtaa cagatggcgc acgacgaaca atggctcacc ccacgtctgc aaacggcggc
     5341 aacgctgtgt aaccagacgc ccgccgcgac ggaatctccg ctgtggctgg gggtggatct
     5401 gggcacctgc gatgtggtgt cgatggttgt tgaccgcgac ggtcagccgg tggcggtatg
     5461 cctcgactgg gccgatgtgg tccgcgacgg catcgtctgg gatttcttcg gcgctgtcac
     5521 cattgttcgt cgccatctcg acacgctcga gcagcaattc ggtcgccgtt ttagccatgc
     5581 ggcgacttca tttcctccag gtacggaccc gcgtatttcc atcaacgtgc tggagtctgc
     5641 tggactggaa gtgagccatg tgctggatga gccaaccgca gtcgcggatc tgctgcaact
     5701 ggacaacgcc ggtgtagtgg atatcggcgg tggcactacc ggcattgcca tcgtgaaaaa
     5761 gggcaaggtg acgtactcgg cggatgaagc caccggcgga catcacatct ctctgactct
     5821 cgccggaaat cgtcgtattt cgctggaaga ggcagagcag tacaagcgcg gtcatggtga
     5881 cgagatttgg cccgcggtga aaccggtgta cgaaaaaatg gcggacattg tcgcccggca
     5941 tattgaaggg cagggcatta ctgatttatg gctggcgggc ggctcttgta tgcaaccggg
     6001 cgtggcggaa ctgtttcgca aacaattccc ggcgttacag gtgcatttac cgcagcacag
     6061 tttgtttatg accccgctgg cgatcgccag tagtgggcga gagaaagcgg aggggctata
     6121 tgcaaaatga attgcagacc gcgctctttc aggcgttcga tactctgaat ctgcaacggg
     6181 taaaaacatt tagcgttcca ccggtgacgc tttgcggtcc gggctcggtg agcagttgcg
     6241 gacagcaagc gcaaacgcgt gggctgaaac atctgttcgt gatggcagac agctttttgc
     6301 atcaggcagg gatgaccgcc gggctgacgc gcagcctggc tgttaaaggc atcgccatga
     6361 cgctctggcc atgtccggtg ggcgaaccgt gcatcaccga cgtgtgtgca gccgtggcgc
     6421 agttgcgtga gtcaggctgt gatggggtga tcgcatttgg cggcggctcg gtgctggatg
     6481 cggcgaaagc cgtggcgttg ctggtgacga acccggatag cacgctggca gagatgtcag
     6541 aaaccagcgt tctgcaatcg cgcctgccgt tgattgccat tcccactacc gccggaaccg
     6601 gctctgaaac caccaacgta acggtgatta tcgatgcggt gagcgggcgt aagcaggtgt
     6661 tagcccatgc ctcgctgatg ccggatgtgg cgattctcga cgccgcattg accgaaggtg
     6721 tgccgtcgca tgtcacggcg atgaccggca ttgatgcgtt aacccatgcc attgaagcat
     6781 acagcgccct gaacgctaca ccgtttaccg acagtctggc gattggtgcc attgcgatga
     6841 ttggcaaatc gctgccgaaa gcggtgggct acggtcacga ccttgccgcg cgcgagagca
     6901 tgttgctggc ttcatgtatg gcgggaatgg cgttttccag tgcgggtctt gggttgtgcc
     6961 acgcgatggc gcatcagccg ggcgcggcgc tgcatattcc gcacggtctg gcgaacgcca
     7021 tgttgctgcc aacagtgatg gaatttaacc ggatggtttg tcgtgaacgc tttagtcaga
     7081 ttggtcgggc actgcgaact aaaaaatccg acgatcatga cgctattaac gcggtaagtg
     7141 agctgattgc ggaagttggg attggtaaac gactgggcga tgttggcgcg acatctgcgc
     7201 attacggtgc atgggcgcag gccgcgcagg aagatatttg tctgcgcagc aacccgcgta
     7261 ccgccagcct ggagcagatt gtcggcttgt acgcagcggc gcaataaatg ccggatgcga
     7321 cgcttgccgc gtctgatccg gccttcggga ttgcacatgt agggcggata aggcgtttac
     7381 gccgcatccg ccaataaata acaactaaca gggagtaaag gcgatgggaa ttaacgaaat
     7441 catcatgtac atcatgatgt tctttatgct gatagctgcc gtagacagga tcctgtcgca
     7501 gttcggcggt tctgctcgtt tcctcggtaa gttcggtaaa agtatcgaag gatcaggcgg
     7561 tcagttcgaa gaaggcttta tggcaatggg cgcactgggc ctggcgatgg tcggtatgac
     7621 cgcgctggca ccggtactgg ctcacgtact cgggccggta attattccgg tttacgaaat
     7681 gctcggcgct aacccatcga tgttcgccgg aacactgctg gcgtgcgata tgggcggctt
     7741 cttcctcgcc aaagagctgg cgggcggcga cgtagcagcg tggctatact ctgggttaat
     7801 tctcgggtcg atgatggggc caacgattgt gttttccatt ccggtggcgc tcggcattat
     7861 cgaaccttct gaccgtcgtt atctggcgct cggcgtgctg gcgggcattg tgaccattcc
     7921 gattggctgt attgctggtg gtctggttgc tatgtactcc ggtgtgcaga tcaacggtca
     7981 gccggtggaa ttcactttcg ccctgatcct gatgaacatg atcccggtga tcattgttgc
     8041 gattctggtg gcgctggggc tgaaattcat cccggaaaaa atgatcaacg gcttccagat
     8101 cttcgccaaa ttcctcgttg cattgatcac cctcggtctt gccgctgcgg tagtgaaatt
     8161 cctgcttggc tgggaactga tccccggtct ggatcctatc tttatggccc ctggcgataa
     8221 acccggtgag gtgatgcgcg ccattgaagt tatcggttct atctcctgcg ttctgttagg
     8281 ggcgtatccg atggtgctgc tgctgacccg ctggtttgaa aaaccgctga tgagcgtcgg
     8341 taaagtgctg aatatgaaca acatcgcggc agccggcatg gtggcaacgc ttgccaacaa
     8401 catcccgatg ttcggcatga tgaagcagat ggatacccgc ggcaaagtca tcaactgcgc
     8461 cttcgccgtt tccgctgctt tcgccctggg cgaccactta ggcttcgccg ctgccaacat
     8521 gaacgccatg atcttcccga tgattgtcgg caagttgatc ggcggcgtaa cggcgattgg
     8581 cgtggcgatg atgctggtgc caaaagaaga cgcgaccgcg actaaaaccg aagcggaggc
     8641 acaatcgtga acactcgcca gctattgagc gtcggtatcg atatcggcac caccaccacc
     8701 caggtgattt tctcccacct ggagctggtt aaccgtgcgg cggtgtcgca ggtgccgcgc
     8761 tacgaattca ttaaacgcga aattagctgg caaagtccgg tgttctttac ccctgtcgat
     8821 aaacagggcg gtttaaaaga agcggaactg aaaaccttaa tactcgagca atatcaggct
     8881 gcgggtattg cgccggaaag cgttgattct ggtgccatca tcatcaccgg tgaaagcgcg
     8941 aaaacccgca atgctcgccc ggcggtgatg gcgctctctc agtcgctggg cgattttgtc
     9001 gttgccagcg ccgggccgca cctcgaatcc gtgatcgccg gtcacggtgc cggggcgcaa
     9061 accctttctg aacaacggct gtgtcgggta ctgaatatcg acatcggcgg tggcaccgcg
     9121 aactacgccc tgttcgatgc cggaaaaatc agcggcactg cctgcctcaa cgtcggcggt
     9181 cgcctgctgg aaaccgacag ccaggggcgc gtggtttacg ctcataaacc ggggcagatg
     9241 attgtggatg agtgcttcgg tgcaggcacc gacgcccgtt cgctgaccgg cgcgcagctg
     9301 gtgcaggtta cccggcggat ggcagagctg attgtcgaag tgattgacgg aacgctttcg
     9361 ccgctcgcgc aggcattaat gcaaaccggt ttgctgcccg caggtgttac gcccgaaatc
     9421 attacgcttt ctggcggcgt gggcgaatgt tatcgccacc agcccgccga tccgttctgt
     9481 tttgccgata ttggcccgct gctggcaacg gcgctgcatg accatccgcg cctgcgtgag
     9541 atgaatgtgc agtttccggc gcaaaccgta cgcgccacgg tgattggcgc gggcgcacat
     9601 accctttcgc tctctggcag cacaatctgg ctggagggcg tacaactgcc gctgcgcaat
     9661 ttgccggtgg cgatcccaat tgatgaaacg gatctggtga gtgcctggca acaggcgctg
     9721 attcagctgg atcttgatcc caaaactgac gcgtacgtgc tggcgcttcc cgcctcgctg
     9781 cctgtgcgtt acgccgcggt actgacggtc atcaacgcgc tggtcgattt cgtcgcgcgt
     9841 tttccgaatc cgcatcccct gctggtggtg gccgggcagg actttggtaa agctctgggc
     9901 atgttgttgc gcccacagct acaacaactc ccgttggcag tcattgacga agtgattgtc
     9961 cgcgcggggg actatatcga cattggtacg cctctttttg gcggatcggt tgtgccggtg
    10021 acggtgaaat cactcgcatt tccttcctga gggaacgact tatgaaacta aagaccacat
    10081 tgttcggcaa tgtatatcag tttaaggatg taaaagaggt gctggctaaa gccaacgaac
    10141 tgcgttcggg ggatgtgctg gcaggcgttg cagcggcaag ttcacaggag cgcgtggcgg
    10201 caaagcaggt gttgtcggaa atgaccgtag cggacatccg caataatccg gtgattgcct
    10261 atgaagatga ctgcgtgacg cggctgattc aggacgatgt taacgaaacg gcctacaacc
    10321 agattaaaaa ctggagcatc agcgaactgc gtgagtatgt gctgagcgat gaaaccagcg
    10381 tggacgacat tgcctttacc cgcaaagggc tgacctcgga agtggtcgcg gcggtagcga
    10441 agatttgctc caacgcggac ctgatctacg gcgcgaagaa aatgccggta atcaaaaagg
    10501 ccaataccac catcggtatt ccgggcacct ttagcgcccg tttgcagcca aatgacaccc
    10561 gtgacgacgt gcaaagtatc gccgcgcaaa tctacgaagg gctttccttc ggagtgggcg
    10621 atgcggtgat cggcgttaac ccggtgacag acgacgtgga aaacttaagc cgcgtgctgg
    10681 ataccattta tggcgtgatc gacaaattca acatcccaac tcagggctgc gtactggcgc
    10741 acgtcaccac ccagatcgaa gcgattcgtc gcggcgcacc aggcgggctg attttccaga
    10801 gtatctgtgg cagcgaaaaa gggctgaaag agtttggcgt ggaactggcg atgctcgacg
    10861 aagcgcgcgc agtgggtgca gagttcaacc gtatcgccgg ggaaaactgc ctctacttcg
    10921 aaaccggaca aggctctgcg ctatccgctg gcgctaactt cggcgctgac caggtgacga
    10981 tggaagcacg taactatggg ctggcgcgtc attacgatcc gtttatcgtc aacaccgtgg
    11041 tcggctttat tgggccggag tatctctaca acgaccgcca gattatccgt gctggcttag
    11101 aagatcactt tatgggcaag ctgagcggca tctctatggg ctgtgactgc tgctacacca
    11161 accacgctga cgctgaccag aacctcaacg aaaacctgat gatcctgctc gccaccgcag
    11221 gctgcaacta catcatgggg atgccgctgg gtgatgacat catgctcaac taccagacca
    11281 ccgcattcca cgataccgcc actgtgcgtc agttactcaa cctgcgcccg tcaccagagt
    11341 ttgaacgctg gctggaaagc atgggcatta tggcaaacgg tcgcctgacc aaacgggcgg
    11401 gcgatccgtc actgttcttc tgatgacgcg gagataacac atcatggatc aaaaacagat
    11461 tgaagaaatt gtacgcagcg tgatggcgtc aatgggacaa acggcccccg cgccgtcaga
    11521 agcaaagtgc accaccacca cctgtgcggc accggtgacc tcggaaagct gcgcgctgga
    11581 tttaggttct gcggaagcaa aagcgtggat tggtgttgaa aacccgcatc gcgcagacgt
    11641 attaacagaa ctgcgtcgca gcaccgtggc ccgcgtttgt accggtcgtg ccggtccgcg
    11701 tccgcgtacg caggcgttgc tgcgtttcct ggccgatcac tctcgttcga aagataccgt
    11761 actgaaagaa gtgccggaag agtgggtgaa agcgcagggc ttgctggaag tacgctccga
    11821 aattagcgac aaaaatctct acctgactcg cccggatatg ggccgccgcc tgtgtgcaga
    11881 agctgttgaa gcgctgaaag cacagtgtgt tgctaatccg gacgtacagg tcgttatttc
    11941 tgatggcctg tcaactgatg ccatcaccgt gaactacgaa gagatcctgc cgccgctgat
    12001 ggcgggcctg aaacaggccg ggctgaaagt cggcacgcca ttctttgttc gttatggtcg
    12061 cgtgaagatt gaagatcaga tcggtgagat cctcggcgcg aaagtggtga tcctgctggt
    12121 gggggaacgt ccggggttag ggcagtcaga aagcctctcc tgttacgccg tttactcgcc
    12181 gcgtatggcg accaccgttg aggccgatcg cacctgcatc tctaacattc accagggcgg
    12241 cacgccgccg gttgaagccg cagccgtcat tgtggatttg gcgaaacgta tgctggagca
    12301 gaaagcatcc ggcatcaaca tgacccgata aggaggcatc atgccagctt tagatttgat
    12361 tcgaccgtcg gtaaccgcca tgcgggtgat tgcctccgtt aacgctgaat ttgcgcgtga
    12421 actaaaattg ccgccgcata ttcgtagcct cgggctgatt tctgctgatt ccgatgacgt
    12481 cacctacatt gccgctgatg aagcgaccaa gcaggcgatg gtggaagtgg tatatggtcg
    12541 ctcgctgtat gcgggcgctg cacacggccc gtcaccgact gccggtgaag tgctgattat
    12601 gcttggtggg ccaaacccgg cggaagtgcg tgccggtctg gatgcgatgg ttgcacatat
    12661 tgaaaatggt gcagcattcc agtgggccaa tgacgcagaa aacaccgcct tcctggcgca
    12721 tgtggtttcg cgtaccggtt cttatctctc atcaaccgcc gggatcacgc ttggcgatcc
    12781 gatggcatat ctggtggcac cgccgctgga agcgacctat ggcattgatg cagcgttgaa
    12841 atctgccgac gtacagctgg tgacctatgt cccgccaccg tctgaaacca actactcggc
    12901 agcattttta accggtagcc aggccgcgtg taaagcagcc tgtaacgcct ttaccgatgc
    12961 agtgctggaa atcgcgcgta atccaatcca gcgtgcgtaa cggaggttgc cgatgatcaa
    13021 tgcactggga ttgctggaag tggacggcat ggtcgccgcg atagatgcag cggatgccat
    13081 gctcaaagca gctaacgttc gtctgctcag tcacgaagtg cttgaccctg gtcgcttaac
    13141 gctggtggtg gaaggcgatc tggcggcgtg tcgtgcggcg ctggacgcag gttgtgctgc
    13201 cgcgatgcgt accgggcgtg tcatcagccg caaggagatc ggtcggccag acgatgacac
    13261 ccagtggctg gttactggct ttaaccgcca gccgaagcaa cccgtaaggg aacccgacgc
    13321 gccagttatc gtcgcggaat ctgctgacga gttgttggcg ctgttaacat cagtacgtca
    13381 gggaatgacg gcaggagaag tggctgccca ctttggctgg ccgctggaaa aagccagaaa
    13441 tgcgctcgaa cagctctttt ctgccgggac gttacgtaaa cgcagtagtc gttatcgtct
    13501 caagccccat taacctgtcg gaggtgccgg gtgtcctaca acacccggca ttaacatcat
    13561 gaaaaagacc cgtacagcca atttgcacca tctttatcat gaacccttac ccgaaaacct
    13621 gaagctcacg ccgaaggtcg aagtggataa tgttcatcaa cgacagacaa cggatgtcta
    13681 tgaacatgct ttaacgatta ccgcctggca gcagatttac gatcagctgc atccgggcaa
    13741 gtttcatggt gaatttacgg aaattctact cgatgatatt caggtttttc gtgaatacac
    13801 cggtctggcg ctgcgtcagt cgtgcctggt ctggccgaac tcgttctggt ttggcattcc
    13861 ggcgacgcgc ggtgagcagg gatttatcgg ttcgcaatgt ctgggaagcg cggaaatcgc
    13921 cacccgccct ggtggcactg aatttgaact gagcacgccg gatgattaca cgatcctggg
    13981 cgtggtgctt tctgaagatg tcatcacccg gcaggctaac tttttgcata acccggatcg
    14041 ggtattacat atgctgcgta gccagtcggc gctggaagtg aaagagcagc ataaagccgc
    14101 gctgtggggc tttgtccaac aggcgctggc gacgttttgc gagaacccgg aaaatctcca
    14161 tcagcctgca gtgcgaaaag tgctggggga taatttgcta atggcgatgg gggcgatgct
    14221 ggaagacgcg taaccaatgg tgacggcgga aagcatcagt catcagagtt accgtagatt
    14281 actttcccgc gcccgtgaat atgtgctgga aaatatgtct gagccggtga cggtgctgga
    14341 cttgtgtaat caactgcatg tcagtcgccg cacgctacaa aacgcgtttc acgctatttt
    14401 aggcattggc ccaaacgcgt ggctgaaacg cattcgcctg aacgccgtac gccgcgaact
    14461 gataagcccg tggtcgcaaa gcacaacggt aaaagacgcc gccatgcagt ggggattctg
    14521 gcatctgggg caatttgcca cggattacca gcagctgttt gccgagaagc cgtcgttgac
    14581 gttgcatcag cggatgcggg aatgggggtg agggagttac acccaatccc tgaccttaat
    14641 aaactcactt aacgccgctt ctgggctgcc atcttttggc tgataatcat actcccagcg
    14701 taccagtggc ggcattgaca tcaggatcga ctcggtgcgc ccgccagttt gcaggccaaa
    14761 cagagtgccg cgatcccaga ccagattgaa ctcgacataa cgaccgcgac ggtagagctg
    14821 aaaattgcgc tcgcgctcgc cgtaggccat cgctttacgt cgctctacaa ttggtaaata
    14881 agcgtcggtg tagcctttgc ctaccgcctg cataaaggca aaacagtggt cgaaatctgg
    14941 cgtgttcaga tcatcaaaga acagcccgcc aataccgcgc tgttcgttgc gatgtttgag
    15001 gtagaagtaa tcgtcgcacc actttttgta acggggataa acgtcttcac caaatggcag
    15061 gcacaggtca cgggcggtgc ggtgccagtg aatggcgtct tcttcaaaac catagaaagg
    15121 ggttaaatcg aagccgccgc caaaccacca gacgggctct gcacccggtt tttcggcaat
    15181 aaaaaaacgc acattcgcgt ggctggtggg aacatacggg ttatgcggat gcactaccag
    15241 tgaaacgccc atcgcctcga aactgcgccc ggcaagctcc gggcgatgag cggtggcgga
    15301 agcaggcatc gcctcgccgt ggacgtgaga aaagttgacg cctgcctgtt cgaaaacacc
    15361 accattacgc aacacccgac tacgcccgcc gccgccagct tcgcgctgcc aactatcttc
    15421 gacaaattct gcgccatcga cggcggtcag ctgctgacaa atcgtatcct gaaggttgag
    15481 cagaaactgt ttaacctggt gtgcgtcggg tttcataact tatcgctttt tcgaatgtgc
    15541 tttctggttg tcgaaccagt ggaaataact gatcacgcct tcagcaatcg ctgtggcgat
    15601 tttctgacga aacgccgccg tgcctaacag ccgctcttct tccgggttgg tgataaacga
    15661 ggtttccacc agcaccgaag gaaccgacgg cgatttcaac accacaaatg ccgcttgttc
    15721 ggtgttgcgg ctgtgcagtt tatgcaccgg cttaatcttc ttcagaatat gcgagccgag
    15781 cgtcagacta tttttaatgg tatctgtttg caccagatca aacagcactt gttgcaatag
    15841 gtgatccttg tcagtcgcct ttttaccggc aacttcatcg gcgcggtttt cgcgttcaga
    15901 caggtatttc gccattgcgc tacttgcccc acggttagag agggcaaata ccgaagcacc
    15961 ggcagctttc gggttggtaa agccatcggc atgaattgac ataaacagat ctgcgccatg
    16021 tttatgggcg atttcaacgc gatcgtaaag tgggataaac gtatcgccag aacgcgttaa
    16081 acgcgcatca atcccatgat tacgcaaaat ggaacggacg tttttagcaa tcgccagcac
    16141 cacatgtttt tctttcgaac cgttgcgtcc gatcgctccg gtatcaattc cgccgtgacc
    16201 tggatcgaga acaacgacac gtttgccgcc agattttttg gctttcggct tgctgtgtcc
    16261 gttgctggtt tttaaaagtt cgtctttggc gatggcttgc gacattcctg acaacgtcag
    16321 ggcagccaaa ccggctttca gcacctggcg gcgcgaagtg agtgttttta gtggtttaaa
    16381 agtgctcata cggcctgagt tgtaataata aagttccaga tgttatatcg tatcgcgtaa
    16441 tccgttacga cggtttgatg ttgagaattg ttttactttt catttcaata gttgacaact
    16501 ccccattatg ccactttttc aaccgatttt cgtcagatat tggctaattc gaccgttcac
    16561 gccataatca gccttttaac ccgcaaaaag gtaacgaata ccatggagat acgcgtattt
    16621 cgccaggaag atttcgaaga ggtcatcacc ctttgggagc gttgcgactt gctgcgtccg
    16681 tggaacgatc cggaaatgga catcgagcgt aagatgaacc atgacgtcag tttgtttttg
    16741 gtcgctgagg taaacggtga agtggtcgga acggtgatgg gcggttatga cgggcatcgc
    16801 gggtctgctt attatcttgg cgtgcatcca gagtttcgtg ggcgtgggat tgccaatgcg
    16861 ttgcttaatc ggctggagaa aaagctgatt gctcgtggct gcccgaaaat tcagatcaac
    16921 gtgccggaag ataacgacat ggtgctcgga atgtatgaac gcctgggata tgaacacgcc
    16981 gacgtgctga gtctgggtaa gcgtttgatt gaagatgaag agtactgagt ttcatcctgt
    17041 ccattatgat gcgcacggtc gcctgcgttt acccttgctc ttctggcttg tgctattgct
    17101 tcaggcgcga acctgggtgc tgtttgtcat tgccggtgcg tcgcgcgagc agggtactgc
    17161 gctgctaaat ctgttttatc ccgatcacga taatttctgg ctggggctga ttcctggcat
    17221 tccggcggtg ctggcgtttt tgttgagcgg tcggcgggct acgtttcctc gcacctggcg
    17281 tgtactctat tttctgttgc tgttggcgca ggtggtttta ctttgctggc aaccgtggct
    17341 gtggctgaac ggtgaatccg ttagcggtat cggtctggcg ctggtagtgg cggatattgt
    17401 ggcgttaatc tggctgctga ccaatcgacg tttacgcgct tgttttaatg aagaaaaaga
    17461 ataacggcac tttttggtga atttgcactc caagcaacgt tattgaataa ccaaaggaag
    17521 tgacatgaaa tcgctgcgtt taatgttatg cgctatgccg ttgatgctga ccggctgttc
    17581 cacgatgtcg tcagttaact ggtcggccgc taacccgtgg aactggtttg ggtcgtccac
    17641 caaagtgagc gagcagggcg ttggtgaatt aacggcgtcc acaccactgc aagaacaagc
    17701 cattgccgat gcgcttgatg gcgattatcg cctgcgcagc ggaatgaaaa ccgcgaacgg
    17761 caacgtggtg cgcttttttg aagtgatgaa gggcgacaac gtggcgatgg tgattaacgg
    17821 cgatcaaggc acgatcagtc gcattgacgt gctggatagc gatattcctg ctgacaccgg
    17881 tgttaaaatc ggtacaccgt ttagcgacct ttacagcaaa gcatttggca attgccaaaa
    17941 agctgatggt gatgataatc gtgccgtcga atgtaaagcc gaaggcagtc aacatattag
    18001 ctaccagttc agcggggaat ggagtggtcc tgaagggtta atgccttcgg acgataccct
    18061 gaaaaactgg aaagtcagta aaattatctg gcggcgttaa tttgcgtctg aacaaaccgc
    18121 cgccgcgaaa aaacacgtaa aatagcgctc aacaatgcca cggattgcgt ggcattatta
    18181 ttttcaggag gaacaatgtc tcaggttcag agtggcattt tgccagaaca ttgccgcgcg
    18241 gcgatttgga tcgaagccaa cgtgaaaggg gaagttgacg ccctgcgtgc ggccagtaaa
    18301 acatttgccg acaaactggc aacttttgaa gcgaaattcc cggacgcgca tcttggtgcg
    18361 gtggttgcct ttggtaacaa cacctggcgc gctctgagcg gcggcgttgg ggcagaagag
    18421 ctgaaagatt ttccgggcta cggtaaaggc cttgcgccga ccacccagtt cgatgtgttg
    18481 atccacattc tttctctgcg tcacgacgta aacttctctg tcgcccaggc ggcgatggaa
    18541 gccttcggtg actgcattga agtgaaagaa gagatccacg gcttccgttg ggttgaagag
    18601 cgtgacctga gcggctttgt tgacggtacg gaaaacccgg cgggtgaaga gacgcgtcgc
    18661 gaagtggcgg ttatcaaaga cggcgtggat gcgggcggca gctatgtgtt tgtgcagcgt
    18721 tgggaacaca acctgaagca gctcaaccgg atgagcgttc acgatcagga gatgatgatc
    18781 gggcgcacca aagaggccaa cgaagagatc gacggcgacg aacgtccgga aacctctcac
    18841 ctcacccgcg ttgatctgaa agaagatggc aaagggctga agattgttcg ccagagcctg
    18901 ccgtacggca ctgccagtgg cactcacggt ctgtacttct gcgcctactg cgcgcgtctg
    18961 cataacattg agcagcaact gctgagcatg tttggcgata ccgatggtaa gcgtgatgcg
    19021 atgttgcgtt tcaccaaacc ggtaaccggc ggctattatt tcgcaccgtc gctggacaag
    19081 ttgatggcgc tgtaagcgtt tctgattcat tgattaaagg ccaggcagta acattactga
    19141 ctggcctttt tattactttt gctttaacgc cgcatacacc tgatcaacta cccaaccata
    19201 cgttgcaatc ggcagctgac cgctttcgaa catattggga ttcttttgcg gatcggcaac
    19261 cggcgaatgt ggcttgttgc ttaacatcac aatcgccata tgattcaccg gatcgataac
    19321 ggtcaccgtt ccggtccagc cagtgtgacc gtaggtttgc gggcttgcca gcgtgccaaa
    19381 cgtcggcgtc atggtggcat taccattcac gcgccagccg aggccaaaag tggcatcttc
    19441 cttagagctg gtggtgaaca tcttcaccgt ttccgcactg aacagctgca catcgccata
    19501 gcccccgccg ttcagcatcg tttgcattaa caccgcaata tcgccggtat tggaaaacaa
    19561 acctgcgtgc ccggaaacgc cgcccatcga ataaaaggct ttttcatcgt gcacctgacc
    19621 ccagagagtg gaggtgcgga tattcgggaa atggatcacg ccatcgcggg tattgccgtt
    19681 caattccgtg gcggcaattt gctgcggttt aaagcctttc agcagcgggt taaataccgt
    19741 atgtgtcagg ccgagcgggc gataaatcga ctcttcaaca tagcggtcaa gcggctgacc
    19801 ggtgaccgac tcgacgataa atccaagcag catataatcg acatcactgt aaatatgctt
    19861 gctgccgggc tgatactcca gcggcgtgcg cttgatcatc tccagcgtct ggcctttatc
    19921 ctgggaatat aacgcgcccg cgaccgcttt attcggatat tgtgggtccg ccgggaaacc
    19981 gccgctgtga tgcagcaggt cagaaatccg cagcgtgttt ttgcctttga tggtgtcatt
    20041 tggactatcg gcaaaccccg gaatatattt cgcaatccga tcgtcaggat gcagtttgcc
    20101 ttcgctcatc agcttttgca gggcgaagtt cgtggcgtac attttggtgt ttgaggccag
    20161 gtcatacagc gtcccggtgg tggctttgac aggctgttcc atcaacacgc tgccatcgta
    20221 ctttttcgcc gctccccagg ctttgcgata cacaatctga ttatctttaa tgatcagcag
    20281 gtttacgccg ggataaccgg cgtccacttg ctggctaatc cagcgatcca tctggttaag
    20341 ccgttcgacg ttgaaccctg ctttctctgg cgaactttct gtcagaacag ggtattttgc
    20401 ggcgctgacg ctacaactaa cagccagcag agaaagatag agcattgtcc gtttcatgat
    20461 atcagtccag attgacgtta cggcagccaa agagcgtggt gaaaataaac ccgcaaaccc
    20521 atgccaccag aatcccgcca gcataaaccg ccattgccgg aaggatgcct tgtgcggaag
    20581 tcatcagcgg cagcgccacc agaccagacg gcccaaaggc gctgtttaag cccatcggca
    20641 gaccccacca ggctatcagg ccgataaaca aaccgcccgc tgcgccgcct aaacaggcgg
    20701 taataaacgg tttcatgcgg ggcagggtga cgccgtaaat caacggttca ccaacgccca
    20761 gcagaccggg aataatcgcc ccgcgtacct gactgcgtaa tgcactgtgc ggttgcgccc
    20821 gccagtagag tgccagcgcc gcgcccacct ggcccgcgcc tgccattgaa aggatgggaa
    20881 ataagctgtt gaatccctgg ctgtccatta acgcgaggta aacaggaata aagccctgat
    20941 gcacgccaaa taccacggcg atcaggaaca gcccggctaa aaccgcacaa ccgaacggat
    21001 tactgttcag gtgcataaac agccatgaca taccttcgaa taaccagcca cccagcggca
    21061 taatgatcag gtacgcaagc gtagcggtga tcagcagggt gattaacgag gtcagcagca
    21121 tgtcgagatc gtccggcata aagcggcgca ccatgccttc aatgcgggcg caggcccacg
    21181 cggcaatcag cacgccgata atattgccgc gcggatcgat gggcagacca aagaaatcgt
    21241 gaaaaccggc gtagtaaccg gtggttgcag cggggttata accgagcaaa aagagtgcgg
    21301 caataattgc gccattgacg cccgtgccgc caaatgcctg ggcggcgtta tagcccacca
    21361 gaatcaccag gaaagtgaac aaacctttgc tgaacacctt cataaaattc agcgcgtcgg
    21421 gtagcgtgcc ctgagcatct gccggaacgt gcatcaccgt ggcaattaac gtcgctatcc
    21481 ccagcagcag accggcggca ataaaaccgg ggatcagcgg cgtgaagatg gtggcgaatt
    21541 tggcgagaaa ttgttgcacg ccggaggttt gtttggcttt taactgacgt ttgttctggg
    21601 cggcgatttc tgcggcatcc tgtaccggtg cctcgcccag cagctcgctc atcgcttttg
    21661 cggcacgatg cgctttacca ggcccaaaaa cgacctgcac ctggtcgctg gtcaaaatga
    21721 cgcccttcac gccttccagc gttttgatat tgggatcaac cagtgaactg tcatgtacac
    21781 ccagacgcag gcgcgtcata cagttaccac aactggcgat atttcccggt ccgccgacac
    21841 gggtaaggat ggtgttcaga agttcactgc tgatctcttt ggccatgggt tattccttgt
    21901 ctaaaacctg acgaataaag ccgccgtgtt gatccaggcg ttttttagct tctgcggcat
    21961 cgagattttt cagcaccatc acaatggccg ttttacagtt gcgctcgcag gcaattaacg
    22021 ccgcttccgc ttgttcggca ttacatccgg tggcgttttt aacgatattg acctggcgca
    22081 catgcagttt ttcgttggtg gcgaccacat cgaccatcag gttgccgaac actttgccag
    22141 atttaatcat cagcccggtg gaaagcatat tgagcaccag tttctgcgct gtgcctgctt
    22201 tcattcgcga agaaccagtg accacttcgg cacctacaat cggtgtaatg gcaaactcgg
    22261 cggtggtgga aacggcactc cccggattac aggaaattcc cactgtgcgg cagccgagct
    22321 ggcgtgcgta ttccagtccg gcaatcacat acggcgtgcg accgctggca gcaatgccaa
    22381 ccaccacatc ctgtgccgtt aaattaatat ttttcagatc gttaacacca ccttcccggc
    22441 tatcttcagc gccttccacc gcgtgctgaa tggcatattc gcctccagca atcaaaccaa
    22501 ccaccagacc cggtttcacg ccgtaggtgg gcggacattc gctggcatcc agaatcccca
    22561 gacgaccgga tgttcccgca ccgagataaa tcagacgacc gccgccgctg acctgggcgt
    22621 ggataacatc gatcgccgcg gcgatatccg gcaggacgcg ctcaacggca agcggtacgg
    22681 ttttatcttc atcgttgata atccggcaca tttccagcgt cgatacgcgg tcaatttcag
    22741 ccgaggcggt gttcgagcct tcagtaatca tcttttctag ttgcatcggt ggtgacctta
    22801 ctatttacga tcaaataatt gcagatgtat ggaatcatat atcccgaatt gacagactta
    22861 aaggaatcat tgagtctaca ctcgcagagt gtgaagagcg tcataagaac ttaagcggaa
    22921 tttatgctgc cagcccagga gagtggaaga tgttgtacct gacgaaaata agcaatgcgg
    22981 ggagtgaatt caccgaaaac gagcaaaaaa tcgctgactt tctgcaggcc aacgtgagtg
    23041 agctgcaatc cgtttcttca cggcagatgg cgaaacagct cggcattagc cagtcgagca
    23101 tagtgaaatt tgcccaaaaa ctcggcgcgc aaggatttac tgaattgcgt atggcgttaa
    23161 taggcgaata cagtgccagc cgggagaaaa cgaatgccac ggcgctgcac ctgcacagtt
    23221 cgattaccag tgatgattcg ctggaagtga ttgcccgcaa attgaatcgc gaaaaagagc
    23281 tggcgctgga gcaaacctgt gcgttgttcg actacgcgcg gctgcaaaaa atcattgagg
    23341 tcatcagtaa agcaccgttt atccagataa cgggcctggg cggatcggcg ctggtggggc
    23401 gcgatctgtc gttcaaactg atgaaaattg gctatcgcgt ggcctgcgaa gccgacacgc
    23461 acgtacaagc caccgtttcc caggcgttga aaaaaggcga tgtacaaatc gctatctctt
    23521 acagcggcag taaaaaagag attgtcttat gcgcagaagc ggcacgaaag cagggggcaa
    23581 cggtgattgc cattacttct ctggctgatt ctccgctgcg gcggctggcg catttcacgc
    23641 tcgataccgt ttccggtgaa accgaatggc gtagctcgtc gatgtctacc cgcacggcgc
    23701 aaaattctgt taccgattta ctgtttgttg gtcttgtgca actcaatgat gtggagtcat
    23761 tgaaaatgat tcagcgcagc agtgagctaa cacaacgcct gaaatgatgc ataaagcagc
    23821 gactggattg agattttcct gaattagtga gctgatccgc agcaatattt tgtttatcct
    23881 gtattttcag agggaatgga gtgtaacgct ctgtattaac aaggagagca ttaaaatggg
    23941 taaactcacg ggcaagacag cactgattac gggcgcattg cagggaattg gcgaaggaat
    24001 tgccagaact tttgcgcgtc atggcgcaaa cctaatcttg ctggatatct cccctgagat
    24061 cgaaaagctg gcggacgaac tgtgtggtcg tggtcatcgc tgtacggcgg ttgtcgccga
    24121 tgtgcgtgac ccggcgtcgg tagccgcagc tatcaaacgc gcgaaggaaa aagaagggcg
    24181 cattgatatc ctggtgaata acgcaggcgt ttgtcgtctg ggcagtttcc tcgatatgag
    24241 cgatgacgat cgcgatttcc atattgacat caatattaaa ggcgtatgga acgtcacgaa
    24301 ggcggtgctg ccggagatga ttgcccgcaa agatggtcgc attgtgatga tgtcttcggt
    24361 cactggcgat atggtggccg atccgggtga aacggcgtac gccttaacga aagcggcgat
    24421 tgttggcctg acaaaatcgc tggcggtgga gtatgcgcag tctggtattc gcgttaacgc
    24481 catatgccct ggatacgttc gcacaccaat ggcggaaagc attgcccgcc agtcgaaccc
    24541 ggaagatcca gaatcggtgc tgactgaaat ggcgaaagca atcccgatgc gtcgcctcgc
    24601 cgatccgttg gaagtcggcg aactggcggc cttcctcgca tcggatgaat ccagctattt
    24661 aaccggtaca cagaatgtga ttgatggcgg cagcacactg ccggagacgg tcagcgtcgg
    24721 tatctgattc acctctgttt cctccctgca tgtgtgggga ggatttcgtc ttattccctt
    24781 ttcaacttcc aaatcaccaa acggtatata aaaccgttac tcctttcacg tccgttataa
    24841 atatgatggc tattagaaag tcattaaatt tataagggtg cgcaatggcc gttaacttac
    24901 tgaaaaagaa ctcactcgcg ctggtcgctt ctctgctgct ggcgggccat gtacaggcaa
    24961 cggaactgct gaacagttct tatgacgtct cccgcgagct gtttgccgcc ctgaatccgc
    25021 cgtttgagca acaatgggca aaagataacg gcggcgacaa actgacgata aaacaatctc
    25081 atgccgggtc atcaaaacag gcgctggcga ttttacaggg cttaaaagcc gacgttgtca
    25141 cttataacca ggtgaccgac gtacaaatcc tgcacgataa aggcaagctg atcccggccg
    25201 actggcagtc gcgcctgccg aataatagct cgccgttcta ctccaccatg ggcttcctgg
    25261 tgcgtaaggg taacccgaag aatatccacg attggaacga cctggtgcgc tccgacgtga
    25321 agctgatttt cccgaacccg aaaacgtcgg gtaacgcgcg ttatacctat ctggcggcat
    25381 ggggcgcagc ggataaagct gacggtggtg acaaaggcaa aaccgaacag tttatgaccc
    25441 agttcctgaa aaacgttgaa gtgttcgata ctggcggtcg tggcgcgacc accacttttg
    25501 ccgagcgcgg cctgggcgat gtgctgatta gcttcgaatc ggaagtgaac aacatccgta
    25561 aacagtatga agcgcagggc tttgaagtgg tgattccgaa aaccaacatt ctggcggaat
    25621 tcccggtggc gtgggttgat aaaaacgtgc aggccaacgg tacggaaaaa gccgccaaag
    25681 cctatctgaa ctggctctat agcccgcagg cgcaaaccat catcaccgac tattactacc
    25741 gcgtgaataa cccggaggtg atggacaaac tgaaagacaa attcccgcag accgagctgt
    25801 tccgcgtgga agacaaattt ggctcctggc cggaagtgat gaaaacccac ttcaccagcg
    25861 gcggcgagtt agacaagctg ttagcggcgg ggcgtaactg atgtttgctg tctcctccag
    25921 acgcgtgctg ccgggcttta ccttaagcct cggcaccagt ctgctgtttg tgtgcctgat
    25981 tttgctgctg ccgctctccg cgctggtgat gcaactggcc cagatgagct gggcgcagta
    26041 ctgggaggtg atcaccaacc cgcaggtggt cgcggcctac aaagtaacgc tgctgtcggc
    26101 gtttgtggca tcgattttta acggcgtttt cggtctgctg atggcgtgga tcctaacccg
    26161 ctatcgcttc ccaggccgca cgctgcttga tgcgctgatg gatttaccct ttgcgctgcc
    26221 aacggcagtc gccggtttaa cgctagcctc gctcttttct gtaaacggtt tttacggtga
    26281 atggctggcg aagtttgata tcaaagtcac ctatacctgg ctggggattg cggtggcgat
    26341 ggcctttacc agcattccgt ttgtggtacg taccgtgcag ccggtactgg aagagttagg
    26401 cccggaatat gaagaagcgg cggaaacgct tggtgcaacg cgctggcaga gtttctgcaa
    26461 agtggtgctg ccggagcttt ctccggcgct ggtggcgggc gtggcgctgt cgtttacccg
    26521 tagtcttggt gaatttggcg cggtgatttt tatcgccgga aatatcgcgt ggaagacgga
    26581 agtgacgtcg ctgatgattt ttgtgcgctt acaggagttt gattacccgg cagcgagcgc
    26641 gattgcttcg gtgatcctcg cggcatctct gctgctgctg ttctcaatta acactctgca
    26701 aagtcgcttt ggtcggcgtg tggtaggtca ttaatggcgg aagttaccca attgaagcgt
    26761 tatgacgcgc gcccgattaa ctggggcaaa tggtttctaa ttggcatcgg gatgctggtt
    26821 tcagcgttca tcctgctggt gccgatgatt tatatcttcg tacaggcgtt cagcaaaggg
    26881 ctgatgccgg ttttacagaa tctggccgat ccggacatgc tacacgccat ctggctgacg
    26941 gtgatgatcg cgctgattgc cgtaccggta aacctggtgt tcggcattct gctggcctgg
    27001 ctggtgacgc gctttaactt ccctggacgc cagttactgc tgacgctact ggacattccg
    27061 tttgccgtat cgccggtggt tgccggtctg gtgtatctgc tgttctacgg ctctaacggc
    27121 ccgctgggcg gttggctgga tgaacataat ctgcaaatta tgttctcctg gccgggaatg
    27181 gtgctggtca ccatcttcgt gacgtgtccg tttgtggtgc gcgaactggt gccggtgatg
    27241 ttaagccagg gcagtcagga agacgaggcg gcgattttgc ttggcgcgtc tggctggcag
    27301 atgttccgtc gtgtcacatt gccgaacatc cgctgggcgc tgctttatgg cgtagtgctg
    27361 accaacgccc gcgcgattgg cgagtttggc gcggtgtcgg tggtttccgg ctcgattcgc
    27421 ggcgaaacac tttcgctgcc gttacagatt gaattgctgg agcaggacta caacaccgtc
    27481 ggctccttta ccgctgcggc actgttaacg ctgatggcga ttatcaccct gtttttaaaa
    27541 agtatgttgc agtggcgcct ggagaatcag gaaaaacgcg cacagcagga ggaacatcat
    27601 gagcattgag attgccaata ttaagaagtc gtttggtcgc acccaggtgc tgaacgatat
    27661 ctcactggat attccttcag gtcagatggt cgcgttgctg gggccgtccg gttccgggaa
    27721 aaccacgctg ctgcgcatta tcgccgggct ggagcatcaa accagcgggc atattcgctt
    27781 ccacggcacc gacgtcagcc gcctgcacgc gcgcgatcgc aaggtcggtt tcgtgttcca
    27841 gcattacgcg ctgttccgcc atatgacggt attcgacaat atcgcttttg gcctgacggt
    27901 gctgccgcgt cgcgagcgcc cgaatgccgc agccatcaaa gcgaaagtga caaaattgct
    27961 ggaaatggtc cagcttgcgc atctggcgga tcgttatccg gcgcagcttt ccggcggcca
    28021 gaaacagcgt gtggcgctgg cgcgcgcgct ggctgtagaa ccgcaaattc tgctgcttga
    28081 tgaaccgttt ggcgcgctgg atgcgcaggt gcgtaaagag ctgcgtcgct ggctgcgtca
    28141 actgcatgaa gagctgaaat tcaccagcgt gtttgtgacc cacgaccagg aagaggcgac
    28201 cgaagtagcc gatcgtgtag tggtgatgag tcaggggaat attgaacagg ctgatgcgcc
    28261 gaatcaggta tggcgcgaac cagcgacccg ttttgtactc gaatttatgg gcgaagtgaa
    28321 ccgcctgcag ggaaccattc gcggcgggca gttccatgtt ggcgcacatc gctggccgct
    28381 gggctacaca cctgcttatc agggtccggt ggatctcttc ctgcgcccgt gggaagtgga
    28441 tatcagccgc cgtaccagtc tcgattcgcc gctgccggta caggtactgg aagccagccc
    28501 gaaaggtcac tacacccaat tagtggtgca gccgctgggg tggtacaacg aaccgctgac
    28561 ggtcgtgatg catggcgacg atgccccgca gcgtggcgag cgtttattcg ttggtctgca
    28621 acatgcgcgg ctgtataacg gcgacgagcg tatcgaaacc cgcgatgagg aacttgctct
    28681 cgcacaaagc gcctgatagg ttgagtgaat gttaaacgcc cggaggcgct tcccgcgatc
    28741 cgggcttttt aatggcaagg tttgtaacct gtagacctga taagacgcgc aagcgtcgca
    28801 tcaggcaaca ccacgtatgg atagagatcg tgagtacatt agaacaaaca ataggcaata
    28861 cgcctctggt gaagttgcag cgaatggggc cggataacgg cagtgaagtg tggttaaaac
    28921 tggaaggcaa taacccggca ggttcggtga aagatcgtgc ggcactttcg atgatcgtcg
    28981 aggcggaaaa gcgcggggaa attaaaccgg gtgatgtctt aatcgaagcc accagtggta
    29041 acaccggcat tgcgctggca atgattgccg cgctgaaagg ctatcgcatg aaattgctga
    29101 tgcccgacaa catgagccag gaacgccgtg cggcgatgcg tgcttatggt gcggaactga
    29161 ttcttgtcac caaagagcag ggcatggaag gtgcgcgcga tctggcgctg gagatggcga
    29221 atcatggcga aggaaagctg ctcgatcagt ttaataatcc cgataaccct tacgcccatt
    29281 acaccactac cgggccggaa atctggcagc aaaccggcgg gcgcattacc cattttgtct
    29341 ccagcatggg aacaaccggc actatcaccg gcgtttcacg ctttatgcgc gaacaatcca
    29401 aaccagtaac gattgttggc ctgcagccgg aagaaggcag cagtattccc ggcattcgcc
    29461 gctggcctgc ggaatatctg ccggggattt tcaacgcttc tctggtggat gaggtgctgg
    29521 atattcatca gcgcgatgca gaaaacacca tgcgcgaact ggcagtgcgg gaaggaatat
    29581 tctgtggcgt cagctccggc ggcgcggttg ccggagcact gcgggtggca aaagctaacc
    29641 ctggtgcggt ggtggtagcg atcatctgcg atcgtggcga tcgctacctt tccaccgggg
    29701 tgtttgggga agagcatttt agccaggggg cggggattta agattattgc gcaggcaccg
    29761 ttttatccgt ctgcgcaatt agcgcatgta aaaaaggctg tgcgttctca tcgctcttac
    29821 cggggatctt cacaatatac ggcttcccgg taatcgatga cgacgaagca accttatcaa
    29881 taaactgctc ggcagtgtca atgcggttac gggtattgcc cagcttcaga cgcagatgag
    29941 aaaccgcttc atcgcaggta tgttcatcac cgttgcgcac aaatatcaaa tcctttttct
    30001 gtgctaatcc ctccagcatg gcgttgatgc gggcttcttc atgggcagtt aacttcgcgt
    30061 aagcggggag tgtcattagt agtgtaatga ccagacaaat gattttcttc attgaattac
    30121 cttatccttg ttttgtgata gccgatttga ctgccagaat caggtaaagt ttcggttgga
    30181 atacgatgag gatcatcggc aaggagaaaa aattaaatga gtagtttgtt gttgtttaac
    30241 gataagagca gggcgctgca ggcggatatc gtcgccgtgc agtcgcaggt ggtttacggc
    30301 agcgtgggca acagcattgc cgtgcctgct atcaaacaga acggtctgaa tgtttttgcc
    30361 gtgccgacgg tattgctgag caatacgccg cattatgaca ctttctacgg tggtgcgatt
    30421 ccggatgaat ggtttagcgg ttatttgcgt gcgcttcagg agcgtgatgc gctgcgccag
    30481 ctgcgcgctg taaccacggg ctatatggga acggcatcgc aaatcaaaat ccttgccgag
    30541 tggctgactg cgctacgcaa agaccatcct gacctattga tcatggtcga tccggtgatt
    30601 ggcgatattg atagcggaat ttatgtcaaa cctgaccttc ccgaggcgta tcgacaatat
    30661 ttactgccgc tggcgcaggg aattaccccc aatatctttg agctggaaat cctgaccggt
    30721 aaaaattgcc gcgatctcga cagtgccatt gctgccgcaa aaagtctgct ttcagacaca
    30781 ttaaaatggg tggtgattac cagcgcctcc ggtaatgaag aaaatcagga gatgcaggtg
    30841 gtggtggtca gtgccgacag cgtgaatgtc atttcccatt cacgggtaaa aaccgacctg
    30901 aaagggactg gcgacctgtt ttgtgctcag cttatcagtg gtttgctgaa agggaaggcg
    30961 ttaaacgatg cggtgcaccg tgcggggttg cgcgtactgg aagtgatgcg ctacacccag
    31021 cagcatgaga gcgatgaatt gattttgccg ccgctggcgg aagcataaaa aatggcgccg
    31081 atgggcgcca tttttcactg cggcaagaat tacttcttga tgcggataac cggggtttca
    31141 cccacggtta cgctaccgga cagtttgatc agttctttga tttcgtccat gttggagata
    31201 acaaccggag tcagggtaga cttggctttc tcttccagta gcggcagatc aaattcaatg
    31261 acagtatcgc caactttcac gcgctgacct tcttcagcaa tacgcttgaa gccttcgcct
    31321 ttcagttcaa cggtgtcgat accgaagtgg acgaacagtt caacgccgct atcagattcg
    31381 atagagaacg cgtgattggt ttcaaagatt ttaccaatgg taccgtctac aggcgcgacc
    31441 attttgttac ccgttggttt gatagcaata ccatcaccaa cgattttttc cgcaaaaacg
    31501 acatccggca cgtcttcgat attgacgatc tcgccagaga gcggagcaat gatctcaata
    31561 gttccggtat ccttcttgtc gtcggaaacc agagatttca gtttatcgaa caaacccatg
    31621 atcttctcct aagcagtaaa ttgggccgca tctcgtggat tagcagattg ttttttcttc
    31681 aatgaacttg ttaaccagcg tcattaactc gtccgttgtc ggttgagcaa gagcctgctc
    31741 tgctaacacc ttcgcatctt cgaagttcgt gttacggata atcttcttaa tgcgcgggat
    31801 agaaatggcg ctcatagaga attcgtccag acccatcccc agcaacagaa gtgtagcacg
    31861 ttcatcgcca gcaagctcac cacacatgcc agtccatttg ccttcagcat gagaagcatc
    31921 aataacttgc ttgatcaagt tcagcacgga cggtgacatt ggttggtaaa ggtgtgaaat
    31981 catatcatta ccacggtcaa ctgccagagt gtactgcgtt aaatcattgg tgccgatact
    32041 aaagaaatca acttctttgg ctaaatgacg tgcaattgtt gcggcagccg gtgtttccac
    32101 cattacgccg atttcaattg actcgtcaaa cgctttacct tcgtcgcgca gttcctgttt
    32161 gtagatttcg atctctttgc gcagtgcacg cacttcttca acagagatga tcatcgggaa
    32221 cataatgcgc aatttaccga aagccgaggc acgcaggata gcgcggagct gatcgcgcag
    32281 gatctcttta cgatccatcg cgatacggat agcgcgccag ccgaggaacg ggttctcttc
    32341 tttcgggaag ttcatgtatg gcagctcttt gtcgccgccg atgtccatgg tacgaacgat
    32401 aaccgcctgc gagccacacg cttcagccac tgctttgtaa gcagcaaact gttcttcttc
    32461 agtgggcagc gcgtcgcggt ccatgaacag gaactcagta cgatacagac caacgccttc
    32521 agcgccgtta cgctctgcac cttcaacgtc acgaaccgta ccaatgttag cgcacacttc
    32581 tacctggtga ccgtccagcg taatagctgg cagatctttc agtttagcaa gctctgcttt
    32641 ttcagaagcc acttgctcct gaacagcgcg cattttatca ataacttcgt tggttggatt
    32701 gacgtaaacc tgattattta cggcatccag aatcagatag tcgtcatttt tcacctgaga
    32761 ggtgacgcta ccggtaccca cgatagcagg cagttccaga gaacgcgcca tgatagaggt
    32821 gtgggaagta cggccacccg cgtcggtgat gaaacccagc accttcttca ggttcagctg
    32881 tgcggtttcg gacggcgtca ggtcagcggc aaccagaatg acttcatcct gaatggcgct
    32941 caggtcgata atcttcaggc ccaggatgtt gcgcagcagg cgcttaccta tatcacgtac
    33001 gtcagccgca cgttctttca ggtattcatc atccaactct tccagggcag aagcctgacc
    33061 ttcgataact tcatgagcag ctgcgtcagc tgtcatgtgt ttatctttaa tcagggctat
    33121 gatttcctgc tccagctcct catcttcgag cagcataata tgcccttcaa agatggcttc
    33181 tttttcttca ccgaacgttt caccagcttt cgttttgatc gtttccagct gggctgatgc
    33241 cttggcacga ccgctcagaa aacgttcaac ttcctgatca acctggtcgg cagaaatttt
    33301 tttccggtca atgacaattt cgtcttcttt cagaagcaga gctttaccga aagcgatacc
    33361 cggggatgct aaaatgcctg aaatcataac cctaccttac ttgtgactga tttttaaaag
    33421 aacccgggaa attactcgag ttccgccatc agtttaacca gatgttcaac cgctttctgc
    33481 tcgtcttcgc cttctgcgga gatagtcaca acggtacctt gagtcaggcc cagagtctgc
    33541 agtttaaaca ggcttttcgc gctggcgctt ttgccgttgg aagtcacagt aatttcagaa
    33601 gtgaagccct tagcttcttt tacaaactgg gcagcagggc gggtgtgcag accgttcgga
    33661 gcggtaatgg taacttcttg ctggaacatt gtatttcccc aacttatagg tttagtgttg
    33721 tggaactaaa gtctagcctg gcggttcgac tttagcctgt attgttagcg ccagttttta
    33781 acagacgcga cgcacgaaga gcgcaataca gtccattgct ggcgggtgtt gaccgctgac
    33841 gtttcgtacg tcattaaaca ttatgccgct aaaggaagaa ttgaaccaaa tcataaaatc
    33901 gattcagctt tggcttttcc tgtaacgatt aatttcgcgc atcaaaataa ttagtctggt
    33961 taaataccag ttccgacagg ctgaatcaat gccaggaggg gcgaaagttt gaagcaggcc
    34021 acaaaaaagc acctaaaaag gtgctttttt acgcattttt aacaagctgg cattactgtt
    34081 gcagttcttt ctcagtgaag agatcggcaa acaatgcggt gcttaaataa cgctcacccg
    34141 atgacggtag aataaccaca atattcttgt tggtaaagct ttcatcttct tgtagtttca
    34201 acgccgcagc aacagctgct ccagaagaga tacctgcaag aataccttct tcttccatca
    34261 gacgacgtgc ggtagaaatc gcttcttcat tggtgatgcc aatgacttta tcgaccagct
    34321 tgagatcgag gttagctggg ataaaaccag cgccaatacc ctgaatttta tgcgggccag
    34381 gtttaatctc ttcaccagcc agtgcctggg cgataactgg agaatcggtt ggctcaacgg
    34441 cgacagagat aagatcggtc ttgcctttgg tgcctttaat gtagcggctg acgccagtca
    34501 gcgtaccgcc agtcccaacg ccagcaataa atacatcaac ctgaccgtcg gtatcttccc
    34561 agatctccgg accggtggtt ttttcgtgaa tttcagggtt tgccggattg ctgaattgtt
    34621 gcagcagcag gtatttctct ggattgctgg cgacaatttc ttctgctttt tggattgcgc
    34681 ctttcatgcc tttagcacct tccgtcagca ccaggtttgc acctaacgct ttcagcagct
    34741 tgcggcgttc aatactcatg gtttctggca tggtcagggt gagtttgtaa ccgcgagcgg
    34801 cagctacata ggccagtgca atcccggtat taccgctggt tggttcaacc agttcaacgc
    34861 ctggtttcag cacgccgcgc ttttcggcat cccaaatcat gttggcaccg atacggcact
    34921 taacgctgaa gctggggtta cgagattcca ccttcgccag aatgcgtccg ttaccgatgc
    34981 gattcaggcg aaccagcggc gtgtgaccga tagtcagcga gttatcttca aaaatcttac
    35041 tcatggcctg tccttaactg tatgaaattg ggatacaaca ggtagcatac ccgctcagag
    35101 aatatgcgga agtaaggatt tagcatatct atatacagaa gggaaataat gacatgcaag
    35161 atggaataag gggcggcata agccacccct gtttcacaca aacggtttac aaaataaccg
    35221 gtagattgtt accgccacat cgcgtgttta tcgcgatagc aatcgaccca catcgccgtc
    35281 gcgccacaaa cggcaacggg catgatgaac agattaagca gcgggatcat cgtaaacagg
    35341 ctggttaaag caccaaactg catattggtg attttgcgtg tgcgcagggc ggtgcgcatc
    35401 tctttaaacg gcactttgtg gttatcgaag gggtaatcgc aatactggat ggctaacatc
    35461 caggcgctaa acaggaacca cagtaccggc gcgacggttt gcccaatacc agggattaag
    35521 taaagaatta gcaatacaat tgcgcgcggc agataccagg caaatttttg ccattcgcgt
    35581 ttcatgattc gcggcacatc tttcatgata ccgaaaatcc cagtatctgg cggtgtagcg
    35641 ccagtcaatc gtgcttccag ttgttcagcc aataaaccgt taaacggagc ggcaatccag
    35701 ttagcaatcg tggagaagaa atagccaaac actaacagca cagagatgac agccagaggc
    35761 cacaacagat aactcagcca ttgtagccag tccggaacgt aactcatgag agtcgggatc
    35821 cagacatcga gctgtgtaaa gagccaccag aatgcgcccc ccatcaacaa aatattgacc
    35881 agcagcggta aaataacgaa acgccgaatc ccaggttgcg agacgagctt ccagccttgc
    35941 gcaaaatagt aaaaaccgct gcgtggggca gatgtgaatg atgaaaccat aatcaggatg
    36001 agctcctttt gaccaatccc aggaaaattc tgcgtatttt accgggtaat tgcgcaatgg
    36061 acagttagga tatgttcgaa aaaacagcaa aaagcacgat ttcatctatc tttgtgctgt
    36121 gaaagttaat agtgcacttg cacttgaggt aatcggcaaa tactcttagt gagtaaatgt
    36181 ttgccgtggt ggcaaggtgt tagaacaaca gagaatataa tgatgcagga tttgcgtctg
    36241 atattaatca ttgttggcgc gatcgccata atcgctttac tggtacatgg tttctggacc
    36301 agccgtaaag aacgatcttc tatgttccgc gatcggccat taaaacgaat gaagtcaaaa
    36361 cgtgacgacg attcttatga cgaggatgtc gaagatgatg agggcgttgg tgaggttcgt
    36421 gttcaccgcg tgaatcatgc cccggctaac gctcaggagc atgaggctgc tcgtccgtcg
    36481 ccgcaacacc agtaccaacc gccttatgcg tctgcgcagc cgcgtcaacc ggtccagcag
    36541 ccgcctgaag cgcaggtacc gccgcaacat gctccgcgtc cagcgcagcc ggtgcagcag
    36601 cctgcctatc agccgcagcc tgaacagccg ttgcagcagc cagtttcgcc acaggtcgcg
    36661 ccagcgccgc agcctgtgca ttcagcaccg caaccggcac aacaggcttt ccagcctgca
    36721 gaacccgtag cggcaccaca gcctgagcct gtagcggaac cggctccagt tatggataaa
    36781 ccgaagcgca aagaagcggt gattatcatg aacgtcgcgg cgcatcacgg tagcgagcta
    36841 aacggtgaac tgcttcttaa cagcattcaa caagcgggct tcatttttgg cgatatgaat
    36901 atttaccatc gtcatcttag cccggatggc agcggcccgg cgttattcag cctggcgaat
    36961 atggtgaaac cgggaacctt tgatcctgaa atgaaggatt tcactactcc gggtgtcacc
    37021 atctttatgc aggtaccgtc ttacggtgac gagctgcaga acttcaagct gatgctgcaa
    37081 tctgcgcagc atattgccga tgaagtgggc ggtgtcgtgc ttgacgatca gcgccgtatg
    37141 atgactccgc agaaattgcg cgagtaccag gacatcatcc gcgaagtcaa agacgccaac
    37201 gcctgataca cttaaggcaa attaactcct cttcgaaccc ccgcttgtcg ggggttttta
    37261 gcattgatgg tgcgatatgg aatcaatcga acaacaactg acagaactgc gaacgacgct
    37321 tcgccatcat gaatatcttt atcatgtgat ggatgcgccg gaaattcccg acgctgaata
    37381 cgacaggctg atgcgcgaac tgcgcgagct ggaaaccaaa catccagaac tgattacgcc
    37441 tgattcgcct acccaacgtg taggcgctgc gccgctggcg gctttcagcc agatacgcca
    37501 tgaagtacca atgctgtcac tggataacgt ttttgatgaa gaaagctttc ttgctttcaa
    37561 caaacgtgtg caggaccgtc tgaaaaacaa cgagaaagtc acctggtgct gtgagctgaa
    37621 gctggatggc cttgccgtca gtattctgta tgaaaatggc gttttagtca gtgccgcgac
    37681 ccgtggcgat ggcaccaccg gggaagatat cacgtctaat gtgcgtacta ttcgcgctat
    37741 tccgctgaag ctgcacggag agaatatccc ggcgcgtctg gaagtgcgtg gtgaagtgtt
    37801 cctgccgcag gcggggttcg aaaagattaa cgaagatgcg cgacgcacgg gcgggaaagt
    37861 ttttgctaac ccacgtaatg cggcagctgg ttcactgcgt cagcttgatc cgcgtattac
    37921 agcgaagcga ccgctcactt ttttctgcta tggcgttggt gttctggaag gtggcgagct
    37981 gccggatact catcttggcc gtttactgca atttaaaaag tgggggttgc cggtcagcga
    38041 tcgggtaacg ctttgtgaat cggcggaaga agtgctggcg ttctatcaca aagtggaaga
    38101 agaccgcccg acgctgggct ttgatatcga cggcgtggtg attaaggtca actcactgga
    38161 acagcaggag cagcttggct ttgtcgcgcg tgccccgcgc tgggcggtag cgtttaaatt
    38221 cccggcgcag gagcagatga cctttgtgcg tgacgtcgag tttcaggttg ggcgtactgg
    38281 cgcgattacg cctgttgcgc gtctggaacc tgtccatgtt gctggcgtgc tggtgagtaa
    38341 cgcaacctta cacaatgcgg atgaaatcga acgtcttggt ttacgcattg gcgataaagt
    38401 ggtgattcgc cgcgctggcg acgtgatccc gcaggtggtt aacgtcgtgc tttctgaacg
    38461 cccggaagat acccgtgagg ttgtattccc gacgcattgt ccggtatgtg gttctgacgt
    38521 tgagcgtgtg gaaggtgaag cggttgcccg ctgtaccggt ggcctgattt gcggagcgca
    38581 gcgtaaagag tcgctgaaac actttgtttc ccgccgtgcg atggatgttg acggaatggg
    38641 cgacaaaatc atcgatcagc tggttgaaaa agaatatgtc catactccgg cagatctgtt
    38701 caaactcacc gcaggcaaac tgaccggact ggagcgtatg gggccaaaat cggcacaaaa
    38761 cgtggttaac gcgctagaaa aagcgaaaga aaccaccttt gctcgcttcc tctatgcact
    38821 tggcatccgt gaagtcggcg aggccaccgc agcaggtctg gcggcatatt tcggcacgct
    38881 ggaagcgctg gaagccgctt cgattgaaga gctgcaaaag gtgcctgatg ttggcattgt
    38941 cgttgcatcc cacgttcaca acttctttgc cgaagaaagc aaccgcaatg tcatcagcga
    39001 gctgttggcg gaaggtgttc actggcctgc gccgatcgtt atcaacgcgg aagagattga
    39061 ctgcccgttt gctggtaaaa ccgtggtgct tacgggcagc ttaagccaga tgtcgcgtga
    39121 tgacgctaaa gctcgactgg ttgaactggg cgcgaaagtc gcgggcagcg tgtcgaagaa
    39181 aaccgatctg gtgattgccg gagaagcggc aggttctaaa ctggcgaaag cgcaggaact
    39241 gggcattgaa gtcatcgacg aagcggaaat gctgcgtttg ctgggtagct gagatggaaa
    39301 aagagcagct gattgaaata gccaatacga taatgccgtt tggcaaatat aaagggcgtc
    39361 gcttaatcga cctgccggag gagtatctgc tgtggtttgc ccgcaaagat gaattcccgg
    39421 cagggaagct cggtgagcta atgcaaatca cgctgctgat taaaaccgag gggctgacgc
    39481 aactggtcca gcccctgaag cgtccgcttt aagctttatc ggcgctgctt tcctgctgtg
    39541 cctgtaactg ttcggtctgg cgtttgtatc gacgcgccag caccgcacag accatcaatt
    39601 ggatctgatg gaaaatcatt aggggcagca ccatcatacc gatcaccgat gtggggaaca
    39661 gaatgtttgc catcgggatg ccatttgcca gactcttttt cgaaccacaa aagacgatag
    39721 taatttcatc tgccttattg aagcccagtc ggcgtgccat aaagacgtta actacaatca
    39781 cgatagccag aagaacgcag ctgaccacca cgataaacag caatgatccc cagccaactt
    39841 tatgccagat accattaacg acggcttcgc tgaacgctgt ataaaccacc aacagaatgg
    39901 acgtctggtc agttttcgca atccattttt tattgcgcga cacccagtca ccaatccacg
    39961 gccgggaaag atgccccaac acaaacggca gcagcagttg cagcataatt ttaccgacct
    40021 gctcaaggct gccccctgca ccgtgaacat tcatcaccag accaaccagc aatggtgaaa
    40081 ggaaaatccc cagcaggctg gatgctgacg cagaacaaac cgccgccgcg acgttaccgc
    40141 ccgccattga cgtgaaggcg attgcagact gcacggtagc cgggagaatg cacaagtaga
    40201 gaaaaccgga gtagagcatc gggtcgacat ttaccggttt ccaccaggca aacagtacac
    40261 ccagaatcgg aaacagcacg aaggtgctgc aaattaccca caaatgcagt cgccagtgac
    40321 caccgccagc aataatcgcc tcacgcgaca acttcgcgcc gtgcataaag aacagcaggg
    40381 caatcgctgc ggtggtcaga ttttcaaaga aggggacgaa atcgcctctg gccggaaaga
    40441 aagaggccag caacaccacc gtgatcaggg ttaaggtgaa aggatcgagg atacgaaaaa
    40501 gtttcataaa gactccagat gatcgatggt tctattgtgc ttcttccagt ttgagaaata
    40561 aaattgattt attgcatcca ttcatgaaga aaacagatga attattcttt aaaacaatta
    40621 aaggttttcg tcacagtagc gcaggagaaa agttttagtc gtgcaggaga gcgtatcggc
    40681 ctgagccagt cggcagtgag tcacagtgtg aaggaactgg aaaatcatac tggtgttcgc
    40741 ctgctggaca gaaccacgcg tgaagtggtg cttacagatg cagggcagca gctggctttg
    40801 cgtcttgagc gactgctgga tgaactgaac agcacgttgc gcgataccgg gcgtatgggg
    40861 caacaactga gcggaaaagt tcgggtcgct gccagccaga ccatttccgc gcatcttatt
    40921 ccgcaatgca ttgctgaaag ccatcgccgc tatccagata ttcagtttgt cctgcacgat
    40981 cgtccgcagc agtgggtgat ggaaagtatt cgccagggag atgtcgactt tggcatcgtc
    41041 atcgatccag gcccagtggg cgatctgcaa tgtgaagcga ttctttccga gcctttcttt
    41101 ctgctttgcc atcgcgatag cgctttggcc gtcgaagatt atgtaccctg gcaggcatta
    41161 cagggagcga aattggtgtt gcaggattac gcgtcaggca gccgaccgct gattgacgca
    41221 gcgctggcgc gcaatggtat tcaggcgaat attgtgcagg agattggaca tccggcgacg
    41281 ctgttcccga tggtagccgc aggcatcg
//
LOCUS       DAEVZA010000028        41078 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEVZA010000028 DAEVZA010000000
VERSION     DAEVZA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.028"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..388)
                     /locus_tag="LAF92_001548"
     CDS             complement(<1..388)
                     /locus_tag="LAF92_001548"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="catalyzes the synthesis of 2-methylcitrate from
                     propionyl-CoA and oxaloacetate; also catalyzes the
                     condensation of oxaloacetate with acetyl-CoA but with a
                     lower specificity; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HBI9790574.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTV"
     gene            complement(433..1323)
                     /gene="prpB"
                     /locus_tag="LAF92_001549"
     CDS             complement(433..1323)
                     /gene="prpB"
                     /locus_tag="LAF92_001549"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HBI9790575.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            1562..3148
                     /gene="prpR"
                     /locus_tag="LAF92_001550"
     CDS             1562..3148
                     /gene="prpR"
                     /locus_tag="LAF92_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HBI9790576.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHNATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(3246..3521)
                     /locus_tag="LAF92_001551"
     CDS             complement(3246..3521)
                     /locus_tag="LAF92_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI9790577.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            3668..4339
                     /locus_tag="LAF92_001552"
     CDS             3668..4339
                     /locus_tag="LAF92_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBI9790578.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(4356..4601)
                     /locus_tag="LAF92_001553"
     CDS             complement(4356..4601)
                     /locus_tag="LAF92_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790579.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(5013..5828)
                     /locus_tag="LAF92_001554"
     CDS             complement(5013..5828)
                     /locus_tag="LAF92_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790580.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(6071..7120)
                     /gene="yahK"
                     /locus_tag="LAF92_001555"
     CDS             complement(6071..7120)
                     /gene="yahK"
                     /locus_tag="LAF92_001555"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HBI9790581.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(7207..8163)
                     /locus_tag="LAF92_001556"
     CDS             complement(7207..8163)
                     /locus_tag="LAF92_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9790582.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(8160..9131)
                     /locus_tag="LAF92_001557"
     CDS             complement(8160..9131)
                     /locus_tag="LAF92_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9790583.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(9124..10608)
                     /locus_tag="LAF92_001558"
     CDS             complement(9124..10608)
                     /locus_tag="LAF92_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI9790584.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(10657..11640)
                     /locus_tag="LAF92_001559"
     CDS             complement(10657..11640)
                     /locus_tag="LAF92_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI9790585.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(11894..12304)
                     /locus_tag="LAF92_001560"
     CDS             complement(11894..12304)
                     /locus_tag="LAF92_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBI9790586.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRDEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(12603..13985)
                     /locus_tag="LAF92_001561"
     CDS             complement(12603..13985)
                     /locus_tag="LAF92_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBI9790587.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSLPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(13995..14945)
                     /locus_tag="LAF92_001562"
     CDS             complement(13995..14945)
                     /locus_tag="LAF92_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBI9790588.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(15088..16506)
                     /locus_tag="LAF92_001563"
     CDS             complement(15088..16506)
                     /locus_tag="LAF92_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBI9790589.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(16506..18053)
                     /gene="fdrA"
                     /locus_tag="LAF92_001564"
     CDS             complement(16506..18053)
                     /gene="fdrA"
                     /locus_tag="LAF92_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBI9790590.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(18043..18906)
                     /locus_tag="LAF92_001565"
     CDS             complement(18043..18906)
                     /locus_tag="LAF92_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBI9790591.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(18946..19551)
                     /locus_tag="LAF92_001566"
     CDS             complement(18946..19551)
                     /locus_tag="LAF92_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBI9790592.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            19809..20306
                     /locus_tag="LAF92_001567"
     CDS             19809..20306
                     /locus_tag="LAF92_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBI9790593.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            20398..21330
                     /locus_tag="LAF92_001568"
     CDS             20398..21330
                     /locus_tag="LAF92_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9790594.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(21372..22460)
                     /gene="pdeL"
                     /locus_tag="LAF92_001569"
     CDS             complement(21372..22460)
                     /gene="pdeL"
                     /locus_tag="LAF92_001569"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HBI9790595.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(22955..23029)
                     /locus_tag="LAF92_001570"
     CDS             complement(22955..23029)
                     /locus_tag="LAF92_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790596.1"
                     /translation="MSDILLIVLNIVFIVIAIILVIIC"
     gene            complement(23335..25368)
                     /gene="betT"
                     /locus_tag="LAF92_001571"
     CDS             complement(23335..25368)
                     /gene="betT"
                     /locus_tag="LAF92_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HBI9790597.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            25497..26084
                     /gene="betI"
                     /locus_tag="LAF92_001572"
     CDS             25497..26084
                     /gene="betI"
                     /locus_tag="LAF92_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HBI9790598.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            26098..27570
                     /gene="betB"
                     /locus_tag="LAF92_001573"
     CDS             26098..27570
                     /gene="betB"
                     /locus_tag="LAF92_001573"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HBI9790599.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETLFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            27584..29254
                     /gene="betA"
                     /locus_tag="LAF92_001574"
     CDS             27584..29254
                     /gene="betA"
                     /locus_tag="LAF92_001574"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HBI9790600.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(29949..30083)
                     /locus_tag="LAF92_001575"
     CDS             complement(29949..30083)
                     /locus_tag="LAF92_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790601.1"
                     /translation="MCVTSNILVMFSSFEIFDVISYEVVFIIYLIDFICILSFCEVRV
                     "
     gene            30329..30892
                     /gene="ipbA"
                     /locus_tag="LAF92_001576"
                     /gene_synonym="fimX"
     CDS             30329..30892
                     /gene="ipbA"
                     /locus_tag="LAF92_001576"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HBI9790602.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
     gene            31222..32016
                     /gene="hyxR"
                     /locus_tag="LAF92_001577"
     CDS             31222..32016
                     /gene="hyxR"
                     /locus_tag="LAF92_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HBI9790603.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPASSLYNGFP
                     HIGTLLKASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATQALRKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVIVGKSINSDAISGKVLSGQRKHMSKKRPGVL"
     gene            32013..32153
                     /locus_tag="LAF92_001578"
     CDS             32013..32153
                     /locus_tag="LAF92_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790604.1"
                     /translation="MTQQRCYYHPLRRVRTRYSVGAKIFLLLWAAGVGGTLYSLYSVL
                     AY"
     gene            32170..32931
                     /gene="hyxA"
                     /locus_tag="LAF92_001579"
     CDS             32170..32931
                     /gene="hyxA"
                     /locus_tag="LAF92_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HBI9790605.1"
                     /translation="MRFVLFCPSGIVPAQFEALSTGSVCNITCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKTGMS
                     VERAISVANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            32973..34055
                     /locus_tag="LAF92_001580"
                     /pseudo
     CDS             32973..34055
                     /locus_tag="LAF92_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            34077..35270
                     /locus_tag="LAF92_001581"
     CDS             34077..35270
                     /locus_tag="LAF92_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI9790606.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSL
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
     gene            35454..36119
                     /locus_tag="LAF92_001582"
     CDS             35454..36119
                     /locus_tag="LAF92_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790607.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(36365..37060)
                     /locus_tag="LAF92_001583"
     CDS             complement(36365..37060)
                     /locus_tag="LAF92_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBI9790608.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(37053..38480)
                     /locus_tag="LAF92_001584"
     CDS             complement(37053..38480)
                     /locus_tag="LAF92_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBI9790609.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILSHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(38491..39210)
                     /locus_tag="LAF92_001585"
     CDS             complement(38491..39210)
                     /locus_tag="LAF92_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBI9790610.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(39740..40594)
                     /gene="rclR"
                     /locus_tag="LAF92_001586"
     CDS             complement(39740..40594)
                     /gene="rclR"
                     /locus_tag="LAF92_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HBI9790611.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRLAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            40820..>41078
                     /locus_tag="LAF92_001587"
     CDS             40820..>41078
                     /locus_tag="LAF92_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI9790612.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNL"
BASE COUNT        10609 a        10484 c         9726 g        10259 t
ORIGIN      
        1 agacggtatg cccctctttt tctggcagcg tgcagccgag cgcggaaacg ccggtgcgca
       61 taacatccat cgggtgtgac gccgccggta aggcttccag cacggtacgc acgttagccg
      121 gtaaaccgcg cagggctttc agtttcgttt tgtaggcggc gagttcgtca cgggtcggca
      181 gtttgccgtg gatcagcaga tgcgccactt cttcaaattc gcaatgttcc gccagatcaa
      241 gaatatcgta gccgcggtaa tgcaggtcat tgccgctttt acccacggtg cagagcgccg
      301 tattgcccgc cggaacgcca gaaagtgcca cagatttttt cggtttaatg acatgggtac
      361 tgttttgcag gatcgttgtg tcgctcatat tgtcctcgtc atttgtaggg tcgagatatt
      421 tttcgttgtg ttttatttca cctggctacg ggcaaacagg tcgtcgagct tctcttcgta
      481 ctggtagtag ttgatgcttt cgtacagctc gttgcgggtt tgcatggtat cgatgacgct
      541 tttctgcgtg ccttcctgac gcaggacgtt gtagacatgt tcagcggcgc ggttcatggc
      601 gcggaacgct gaaagcgggt acagcgccat tgcgacatgg gcgctgcgta attcgtcggt
      661 ggtaaacagc ggtgttgcgc caaattcggt aatgttggcg aggattggca cctgcaccgc
      721 atcggcaaac tggcggtaca tggcgagttc ggtaatcgcc tccgggaaca acatctcggc
      781 acccgcttca acataggcct gcgcacgctc gatcgccgca tccagcccct ctaccgccag
      841 agcatcggtg cgcgccatga tcacaaaatc aggatcggtt ttcgcatcca ccgccgcgcg
      901 gatccgatcc accatctctt ctttcgagac gatcgcttta ttcggacgat gaccgcagcg
      961 tttcgcacca acctgatctt caatatgcaa tcctgccgca ccggctttaa tcatcgattt
     1021 cacggtgcgc gccacgttaa aggccgaaga accaaaaccg atatccgcat ccaccagcag
     1081 cggcagcgaa caaacgtcgg tgatacggcg aatgtcggtc agcacatcat caagggtaga
     1141 aataccgaga tcgggcagcc ccagcgaacc tgccgccacg ccaccgccag aaagataaat
     1201 tgcctgatat ccggcacgct gcgccaacag cgcatgatta gcgttgatgg tgccaacaat
     1261 ctgcaatgga ttttctttag tcagtgcagc gcgaaacgct ttacctggag agtgtagaga
     1321 catagcccat cctttgttat caacttgtta tttgcgttga taaagacaaa gcaaggggtg
     1381 tgccacaatt gccttaataa aattaaatgt ttaatttcaa tttgttagag ataaagtcat
     1441 gaactaatcc gcaaatatgc gtttcagtta acgtttcagg caatgtttca ccgcgtttca
     1501 ttgcaacaat tatgaaacaa gactaaaccc aatattcggt ttcttaactt tgcggtgcgc
     1561 tatggcacat ccaccacggc ttaatgacga caaaccggtt atctggacgg tatctgtaac
     1621 gcgcctgttc gagctgtttc gcgatatcag cctcgagttt gatcacctgg cgaacattac
     1681 ccctattcag cttggctttg aaaaagcagt gacctacatc cgcaagaaac tggcaaacga
     1741 acgctgtgac gccatcatcg cggctggctc taacggcgcg tacctgaaaa gccgcctgtc
     1801 agtgccagtt attttgatta aaccgagcgg ctacgatgtg ttacaggcac tggcaaaagc
     1861 cggaaaactc acctcttcta tcggcgttgt cacctatcag gaaaccattc cagcgctggt
     1921 ggcgtttcaa aaaaccttta atctgcgcct cgatcaacgt agctacatta ccgaagaaga
     1981 cgcccgcggg cagattaacg agctgaaagc taacggcact gaagcggtgg tcggcgcggg
     2041 gctgattacc gatctggcag aagaagccgg aatgaccgga atttttatct attccgccgc
     2101 caccgtgcgc caggcgttca gcgatgcgct ggatatgacg cgcatgtcgt tacgccataa
     2161 cactcacaat gccacccgca acgccctgag aactcgttac gtgctgggcg atatgctcgg
     2221 tcaatcacca cagatggaac aagtacggca gactattttg ctgtatgccc gctccagtgc
     2281 agcggtgttg attgaggggg aaacggggac gggcaaagag ctggcggccc aggcgattca
     2341 tcgggaatat tttgcccgcc acgatgcgcg acagggcaaa aagtcgcatc cgtttgttgc
     2401 cgtcaactgc ggggcgatag ccgaatcgct gctggaagcg gagctgtttg gctatgagga
     2461 aggggcgttt accggctcgc gacgcggagg tcgcgccggg ctgttcgaaa ttgcccacgg
     2521 cggtacgctg tttctggatg agattggcga aatgccgcta cctttgcaga ctcgcctgtt
     2581 acgggtgctg gaagaaaaag aggtcacccg cgtcggcggg catcagcctg ttccggtaga
     2641 tgtacgggtc attagcgcca ctcactgcaa gctggaagaa gatatgcagc aaggacgttt
     2701 tcgccgtgat ctgttttatc ggctgagtat tttgcgtctg caattgccac cactgcgcga
     2761 gcgggtggcg gatattcttc cgctggcgga aagctttttg aaagtgtctc tggcggcgct
     2821 ctccgccccg ttttctgccg cattacgcca gggattacag gcaagcgaaa ccgtgctggt
     2881 gcactacgac tggccaggta atattcgtga actgcgcaat atgatggagc gactggcgct
     2941 atttttaagt gtggaaccga cgccggattt aacgccgcaa tttttgcagc tgctactgcc
     3001 ggaactggcg cgcgagtcgg cgaaaactcc cgctccacgc ttactgacac cacaacaggc
     3061 actggagaaa tttaatggcg ataaaacagc agcggcgaat tatttaggca tcagccggac
     3121 gacgttctgg cggcggctga aaagctgaac tgccgaatgc agcgcgaacg ctttatccgg
     3181 cataaatttc aatagatgat caccgataaa cttacgcatc gggcatgttt acatagggtt
     3241 cagaattact tcttcttata aatatttgcc gtgccgtgga tcttattgtc agtttgacca
     3301 gaggtcagta ccaacacatc agcccctttt tcatccgctt ttttgatcaa atcctctttc
     3361 gcatctgcgg tcgacatttc attactggtt gaaatatcac ctattttttc atactgcgat
     3421 tcaacttttt caaattccgc tttggtcatc aattcagcgg catagacatt ggtaacggca
     3481 aacgctaacg caccgactaa cattttagag attattttca tattacggct ccagagttgt
     3541 tatcacacta tcacccaccc acattattgg gttgattaat aattagttca gtcagggcat
     3601 aaacgccagt aaccggaaat aagacacttt gtgatctctc tcgcacccga ttatttttgg
     3661 tagaaatatg atgaagttac ttcacttatt tatggatgaa atcactatgg atcctttgca
     3721 cgccgtttac ctgaccgtag gactgttcgt gattactttt tttaatccgg gagcaaatct
     3781 ctttgtggta gtacaaacca gcctggcttc cggtcgacgc gcaggggtgc tgaccgggct
     3841 gggcgtggcg ctgggcgatg cattttattc cgggttgggt ttgtttggtc ttgcaacgct
     3901 aattacgcag tgtgaggaga ttttttcgct tatcagaatc gtcggcggcg cttatctctt
     3961 atggtttgcg tggtgcagca tgcgccgcca gtcaacaccg caaatgagca cactacaaca
     4021 accgattagc gccccctggt atgtcttttt tcgccgtgga ttaattaccg atctctctaa
     4081 cccacaaacc gttttatttt ttattagtat tttctcagta acattaaatg ccgaaacacc
     4141 aacatgggca cgtttaatgg cctgggcggg gattgtgctc gcatcagtta tctggcgagt
     4201 ctttcttagt caggcgtttt ctttgcccgc tgtgcgtcgt gcttatgggc gtatgcaacg
     4261 cgttgccagt cgggttattg gtgcaattat tggtgtattc gcgctacgcc tgatttacga
     4321 aggggtgacg cagcggtgag cagataattt actctctacg taatcaacct gatttgccga
     4381 atcccggcca tcggcgatga tagcccccgt aatgcgtggt ccttatttcc gataagttta
     4441 cttgagtctt tagcttccca tttcagccca ctctcccctg tgtgggcttt tttcattggt
     4501 ctgacccgtc ctgaatagcc aaacaatggc aatgttccac tttgcaggga tgataataag
     4561 gggatctgat tcagaagagt tttgaaaagt tggaccgcca tcgaggcctc gaaccccgta
     4621 ccatacaaca atcctggttg tcgtctgctc ttcctgatga gctaatggcg gtttttgata
     4681 tgagccttac agcatatttt ctgtcaggct tgagttcgat gctaaggtac agaaaagacc
     4741 ccacacaaac atgatggaat tatgattttt ttcatatgaa tgtcaaccca cacattcaca
     4801 aaacatctgc atgagatcat tttttataca ccgcatagct gcgaaaccac tgctggtgct
     4861 tttttaagcg aagcgtcgcc gatcacaaca aaatatcggc gaaaaaggcg gtggaatgcc
     4921 atttggcata cggatataca gatgaaatga ttacctgctt tgcgattcac tctctggtgg
     4981 aataacaact tgcaggtgga accctttctg aactaaagag tgtagcggat tttaacgtgc
     5041 ttatttacag gataatatct gtcacctaat tcctgcactc tattcgatat ccattcattt
     5101 aatcgtgcat catctctgta tgtttttacg tccagaacag agagcaataa catcaccagt
     5161 gattcaggaa aaacagggta gatatttttt aagtgtaata ataatccatg cgcaagcgcc
     5221 tgcttaatgg gcgcgtctgt ctccggaata gacgacattt cgcgaagcca tttttccaca
     5281 tgcatacact ctttcccttt agggaaaagt ctcagcaaag gcatatggtg ttcaagaaga
     5341 atgcatttgc aatctgttcg cactgaatcg ataaactgca ctaatttctt aagcccggta
     5401 aagcgtccag cctgcacatt tgctttaata tcaagaaacg catcatcctg gtcatatacc
     5461 caaactgtcc ctttttgatt ttcaagtatg cgaaaaacaa acttaaccgg gtgatcaggg
     5521 taatccatcc agtcataaaa tccagggaaa tcattaaact gtacatttat ggtgagtgcg
     5581 ttatcgtctg accactcaca gtctcgtacg gcagccatga gatgaatgag tttgtcgtca
     5641 tcgactaaat catcacgaca atcataagga taatcataat attgtttaat caaaaccttg
     5701 aatatatcga cacaggaaac caaggagctt ttgatatcct ctggtaactg attgttttta
     5761 accgtattaa gtatgctttg ttgcgtatat ggcatcaaat tattgtgcgg agcttttaat
     5821 gatatcatcg cgatctctct taaataaaaa taaacgtcgc agataataaa gctctgatta
     5881 aaccaggcac tatcagaaat cgctcataac taacgtgtga agtattgtgt actggaggga
     5941 gttaatttaa caatttgaat ttacgttata ggttttactg ctactttgat attatttcgc
     6001 tctccttaac taaaaagtta agtggagagc gaagttaata tgttaaacca cagcgtattt
     6061 attaattttt tcagtctgtt agtgtgcgat tatcgataac aaaacgatat ttcacatcac
     6121 cacgcagcat tcgctcatag gcttcattaa tttgatcggc ccgaatcatc tctatatcag
     6181 ccacgatgcc atgttcggcg caaaaatcga gcatctcctg agtttctgga atgccgccaa
     6241 tcatagaacc ggctatcgca cggcgtttca tgatcaggtt gaaaacttcc ggcgatttat
     6301 gcggtgtcgc aggcgcacca accagcgtca tggtgccatc acgcttcagc aaggtggtaa
     6361 aatcgtcgag attatgtggc gcagctactg tattcaaaat gaaatcgaaa ctcttcagat
     6421 gagccgccat ctcatcggca ttgcgtgagt taacaacttc atcggccccc agggcttttg
     6481 ccgcttcgcg ttttgcctca gaagtggtaa atgccaccac atgcgccccc atcgcgtggg
     6541 ccagcttgat ccccatatgt cccagaccgc cgatgccgac cacgcccact tttttacccg
     6601 gcccggcctg ccagtgacgt agcggcgaat acgtggtgat ccctgcacac aacaaaggag
     6661 ccaccgccgc cagctgctct tgcgggtgac gaatacgcag aacatatcgc tcatgaacga
     6721 cgatctgttg tgagtagccg cccagagtat ggcccggttc gtccggcgtc ggcgagttat
     6781 aggtgccggt catgtgatca cagtagtttt ccagcccgtc ttcacactct tcgcaatgtt
     6841 tacaactgtc gacaatgcag ccgacaccga ccagatcgcc cggcgcatgt ttttctacct
     6901 gatcaccaac ggctaccaca cgccccacaa tttcatgacc cggtacgcag gggtaaaccg
     6961 tccccgccca ctcggaacgg acctggtgga gatcggaatg gcagacgcca cagtaagcga
     7021 tttcgatttt gacatcattc ggtcccggtt cacgccgtgt gatatccatc ggttcaagtg
     7081 gttgtttagc ggaatatgca ccaacagctt tgatcttcat tgtgtttact cctgattagc
     7141 tatgtgtatt tggcgacgcc tgatatgtca tcgcggcaaa acgcgtccat tgaatatagc
     7201 caatatttaa accacccgat ccagccccaa catgccgcga acctgaatga aacagagcag
     7261 caacgttccc cataaggcca tcgtcaggta ggggcttata ccgagaatat tgaagccgct
     7321 ttcaataatt tgcagaagaa acagcgccag caccatacca attatccgcc cgctgccgcc
     7381 gtccggatta accccaccga gtactgccgc gaggattgac accagcaagt acgattcgcc
     7441 ataagaggcc ttagccgagt tgagtttcga catcatcaaa aatgccgcta ccgcacaaag
     7501 caaggcggaa atgacataaa cccaaatcag cacccgacga gtgttaatgc cggagtaaag
     7561 tgttgctcgt tcgttagacc caatcaggta aatggcttta ccgagcggcg ttttctccag
     7621 caatatccat agccccagtg ccactgcggc aaacaaccac atcggtagcg gaataccgaa
     7681 ccattgcgca tgattcaacc acagcaccca tgagggataa ttggcaatgg cgctaccacc
     7741 tgtcaccaga atatttatcc ctttgagcaa cgtcatcatc ccaagcgttg caagaattgg
     7801 cgacactcga ataccagcaa tcaatacgcc gttacataaa ccgattatca ccgccgcgcc
     7861 agcgcccgcc agtaacgtag cgacaaccgt agcaataccc ggtggatact gagtcgccac
     7921 ccacgccatc accagtgaac aggcgttagc agtggctata attgacaagt taatgccacc
     7981 gcaaagcatg gtcaccgcca tagccaacgc cagaatgccc agcacgggca tttgcgaagc
     8041 gacggactgg aaattactta tcgaccagaa aatctccggc atagtaatgc tgaacgcggc
     8101 aacagttagc accagcaggc cgatcaagta aaactcaacg ttattacgcc acgatttttt
     8161 catagcagac tccggttctg atgctgactc cacgccgtgg cactaatgct ggcaacgatg
     8221 atgatgccgg tgatcaatgt ttgccagtaa gacgagactc ccaataaatt tagcccgttt
     8281 tgcatcactg ccaacaacac cacgccgagc aaagtaccgg ttaacgtgcc gcgcccgccg
     8341 agcaaactag tgccgccaag caccaccgca gccagtactg tcagctcata acccagaaga
     8401 gaatcggggg cgacagtcat caccgtccac gactgcacta cacccgcagc gccagacatc
     8461 aatcccatat agccatagac gaaaagttgc agtttcagca cgctaaagcc gatgcgactg
     8521 gctgattcgc ggttcccgcc aagggcataa attttgcgcc ctacactggt gaaattcatg
     8581 ataaacgctg ttagcagcac caccgtaatc atggcgatca gcggcagacc aaggccatag
     8641 tcatagccat cagcatcggt gtacttgaac aacataaccc cctgctcaaa ccacggcgga
     8701 aagtcgtaca gccacacacc tttactcaac cataacagca ggccatagaa aatattcagc
     8761 gtcgagatag tgatgatgat agaaggcacc cgtaggcgat taaccagtaa ggcgttaatt
     8821 aagccaagta aaatgccgat gcagcccgcc agtgcaaacg cgacagcgaa gttgccacca
     8881 attttttgca gcaacagcac catgccgtat tgcgcaatga tggtcattgc tggaaacgaa
     8941 atatcaattc caccggaaat caacaccaca aacaaaccac aggcgagaat agtcaacatg
     9001 gcatagttat tggcgagatc gtacaaatta ccgaatgtca gaaactcgtc gctgcgccag
     9061 gcaaggccaa caaacagcac cacaatcagt aatgccagcc agaattcatg gcgttttttc
     9121 agttcagcca tttaccacct ccgcaatcct ggcctgagaa ctgctgtcag gcaggaagct
     9181 atgggtgatc tgaccttttt gcattaccaa aatccggtgg ctttgatacc acgcttcttc
     9241 aatttcatcg cagatcatca ataccgcaat gccatgagcc gccagatcgc tgataatgcc
     9301 gtatattcct gctttgttgg cgatatcaac accgactgtc gggctatcga gaatcaacag
     9361 tcgtggccca atcgccagcc atttggcgat cgatactcgc tgcgcgttac caccagaaag
     9421 cgtattcacc ggaagatgag gatcagagac tttgatggtc aatgccttga ttagcctgtc
     9481 caccaggtca cgaactttag tttcactcag aaagccgaag cgatctttta ccttgtgaaa
     9541 aacagtgcta atgatgttgt cttcaataga ttgcgccatc accaaaccgc gcgacatgcg
     9601 atcttccgaa acataaccaa taccgtggcg gatagcgtcc tggttactat gcagtgtaac
     9661 cggctggccg ttgatgagga tctcgccagc atccggtcgg gtcataccaa acagactcag
     9721 acataattcc gtgcgccccg caccaagcag gccaacaatc gataccactt cgcccgcttc
     9781 cacccgcaag ttaatattta gatattcccc atgacgactt aaattacgca cctcaagcac
     9841 cggggtgctg gattttccct gccacaactc tcgcacctga tattcgaact cctggccggt
     9901 catcaggaaa ccgagttgtt ttgtggtcat ctctgcagcc ggaaaagtcc cgaccagctc
     9961 accatctttc agcactgaaa tacggtcgga aacttccatc acttcttcca gacgatgact
    10021 gacgaagacc acgcagatcc cgcgttcacg caattgatgc accacctgta ataacccttg
    10081 cacttcctga tgggtcagcg aagcggttgg ttcatccatc acgatcaacc gtgcgtcctg
    10141 cgccagtgca cggcaaatag ccaccagctg acagcgggca attgaaagct cagaaacgag
    10201 tgtatccagt ggcaatgtga cgttaatact ggtcattgcc gcctgcgcca cctcacgcag
    10261 acgacggcgg ttaacaaaca gaccgtggtg atagtgattc acggcgatat tctcccagac
    10321 actcaggtta ggaaataaag agaggtcctg ataaatcacc tgaatcccct gcgccaccga
    10381 agcggcgggt gtcagctttg accagttttt cccaccaagc gtaatttccg ctccctcatc
    10441 cgggcgataa acaccggaca taattttaat caacgtcgat ttaccacagc cgttttgccc
    10501 ggcaaggcag tgcacttcac ccgacataaa gtcgagagaa acatcgcgca gcgctcgcgt
    10561 ggcatgaaac gttttgttga tatgacgaag ggaaaggaaa gtttccataa catcccctgg
    10621 caataagaat cggggctttc gccccgaaga aatggattaa taaagtgagt cgatattgtc
    10681 tttgttgacc agtaatacct tatggaaacg gataattcgc ttatccatat ctacgtcggc
    10741 cttacccaga ttctgcattt ctaatccagg tttgatctct tcacccttca gcaacgtgct
    10801 ggcgacggct gcgagtgcgt aacctgcaga ggccggatcg taagtaatcc cttcggtgat
    10861 atcaccactt ttaatcagtg atgccgcctg tgaagggatc atcatgccat agactgcgac
    10921 tttatttttc gcccgtttct ctttcaccgc acgtcccgcg ccaatcggac cgtttgaacc
    10981 aaaggagaca accgctttca agtcaggata ggttttcatc aggtccagtg tggtacgacg
    11041 tgagtcatcc acactctcgg caaccggcat gcggcgggta acttcatgca tatccgggta
    11101 atgctctttc tggtatttca ccagcaagtc agcccataag ttatgctgcg gcacggtcaa
    11161 actacccacg taaatcacat agccgccctt gccccccatg cgtttcgcca tatgctcaac
    11221 atattcagcg gcaaattttt cgttatcaat gatttcaata tcccagttag cacttggctg
    11281 accgggggat tcgttggtca gaaccacaat tccggcatct cgcgcttttt tgaataccgg
    11341 ttccagcacg ttggcatcgt ttggcacgat agtaattgca ttaaccttac gggcgattaa
    11401 atcctcaata attttaactt gttgcggagc atcagtactt gaaggcccca cctgtgaggc
    11461 attaacacca aaggctttac ccgcctcaac cacaccttcg cccatgcgat taaaccacgg
    11521 cataccatcg actttagaaa tattcaccac gactttttcc gctgcctgga gcggcgcaga
    11581 aattagcgca gcgcctaata acagcgaaga caccatattg ataacaaaac gtttattcat
    11641 catttcagcc tcattgcagg gtagtcagga agtgttcgcc caaattagcg cgctggagag
    11701 aataagaatt ttcgaatatt tttatgcaga ataaacagcc ctatttcagg cagctattaa
    11761 aaatatggtt ataaatatgg acaaaacggg cagcaacaat aagtgtgaca ttcatcacgt
    11821 aaaaaatagc aaaataaaca aaattagcga tattttatgt gtgtgagttt atttcgcgta
    11881 gataaatagt tatctacaca acgtcaataa gggcatattc ttcaaatata acgtccattt
    11941 ctggtgaata gcttccttct gcctggaaaa aacgacggtg ttcattacgc caatattcaa
    12001 gactgaggtc accttcacct tccttacggg caagctccac ggtgacatca gaaaagcgca
    12061 gtaaatgcag gccaatcact ctgataacac atacaggctc atcacggcta ttttctacaa
    12121 tcttatactc accaatcatc ggaaatgcgt tgtcttcaat acatcccgcc agcgaaccac
    12181 aggaagctgt tttaacacca gtcgcaatca acgttgcgag gtgatcggcc aacgctgctg
    12241 aatcgccaaa agtacaaaag gaagcatggg gatatttttg tgagaatttt tcttttatgg
    12301 tcatggcaat atttttgcga ttacggaaaa acaaatcaca tcttaattac tgttggtaac
    12361 catcagcaag agtatcattg gtttttcgat actgaatctt ttttatccga agcaggtcac
    12421 atttatggcc tgataaatac ctttgttaaa actcactttt gtgtatttct ctcaaataat
    12481 cccctggaaa ttatgccgcg agattataaa gttcaggatc attcattagc aaaccattcc
    12541 cgccccactc cctggggctt aaatctctta caattcaccg ttgcaggcgt ttcaaatgcg
    12601 catcaacctg ccacactccc ccacaccagt tgccctttat ggaacgttgc ggttctcggc
    12661 gagatacgcg ccaccgcctc ggcggaacag gaggcgtcca ccagcacaaa gctggcatca
    12721 tcctgcgctt ttggccatac acgctcgcct ttttcattca gcggcaatat atcgccagtg
    12781 gctagaaaca gcgaacggga gaggttctgt tcgttaggac gaatatagag ctgcgcgtag
    12841 agattggctt tttccagcat gtcgcccaga ccataaggcg accagtggtc gataacgctg
    12901 tcagtgccag tcatcacttt tacgcctttg tcgtgcaact gtttgagcgg catatgcagc
    12961 gtgccaatcg gcaccgtcga ggcgatagaa atttgctgcg ccgccatccg attcgccagc
    13021 gcatcgacct gttgttcgtt gagcgtagcc agcgcaaagg cgtggctgat ggtcagctta
    13081 cctttcagtt gcggcgtttt ctctaccgtt tcaaccatat aattgatggc tgccacgccc
    13141 gacggagtgg tttcgtgcag gtgaatatcg acacctttgt cgtagtccag cgcaatctga
    13201 aacatggtgt cgagggattt ttccatcgcg ccatcgacac tggtcgggtc cagcccgccg
    13261 acgtaatgcg cacccgcctg catcgcttcg cgcattaagg gttccgattt cgacagcagc
    13321 aaaccgtgct gcgggaaggc gacgatttca cactcgaagc ccgcctgacg ccgcgccagc
    13381 accgcctgca aattttgcag attttttagg ccggaaaccg gttcgatatt gcagtggctg
    13441 cgggcaatgg tggtgccttt cgactgcaat agatcgatca gtttttccgc ccgctcctgc
    13501 gtatacggtt gtaactccgg cagcattttc tgctcaagct tgatcatgtc ctggatagtg
    13561 gtgcctgccg gacgattgag cgagcgccac ggcccgccgt agaaggtttt gtcgagatga
    13621 atatgcatgt cgcgagtggt gggtagcatc aacttaccgc cagcgtcata gtgcggcagc
    13681 gtggcgtccg gatgctgctt gttctcgcgc agggcgacaa ttttgccgtc ctgaatctcc
    13741 acggtctggc gcgccgtgcg ggtctgcacc gccacaccgt tttcgtagtc aaaaccggtt
    13801 tccagcagca cgttatcgag atagtaatgc gggtcagtga ttttcatggt gttgttcgct
    13861 tcgcagttta gggtgccagc taccgccgca tgggcgagcg gcaaagaggt accaaacagc
    13921 gcggcggcgg tgaccatctt accgctctgg ctcagaaatt cacggcggct attgctttct
    13981 ttcatcctac gtccttacgt tttaataata tgggtgcccg tttcaccgcg cagcgccgca
    14041 ggcaggcatt ccggcgtggt gataatcact tctttgccgc cctgctccag gaacgtcagg
    14101 ctggcgatga tttttggcaa catgctgccg ggcggaaaat gcccttcctg catatagcga
    14161 gtcatggtgg caatatccac ccgatccagc gcctgctgct gcggtttacc aaagtggata
    14221 cagacttttt cgacgccggt ggtgatcacc agaatgtcgg cgtgaatttc acgggccagc
    14281 agcgcggtag agagatcttt gtcaataacc gcgtccacgc tttggtaatc ccccgcttca
    14341 gtacgcacta ccggaattcc accgccgccc gcgccaatta cgacaaaacc ttgttggatc
    14401 agcgccttaa tggcgggtgc ttcgacaata cgtttcggtt ccggcgaggc gaccacgcgg
    14461 cgatagcccc gtccggcatc ttcaacaaaa caccagtcag ggtttgcctt ttgtagtttg
    14521 tcacgctggc tttcactgaa gaatgcacca atgggcttgg tgggatgggc aaaacctgga
    14581 tcgtttttat ccacttccac ctgagttacc acggtgacgg cttttttctc gccgtgacgc
    14641 gccagccggt tattcagcgc ctgttggatc agatagccga tgccgccctg cgtatctgcc
    14701 acacagttcg ccagcggcgt taagggcagc ccttcgcgct cgtgggcaat ctccgcacgg
    14761 cgtaaatcca gcccgacctg cggcccgtta ccgtgggtca gcacaatgtc gtaatcggaa
    14821 gccagcattt ccagcaccgt atcggccacg gctttcaccg cctccgcctg atgctcaatc
    14881 gactggctgg cgttatcttt gataatgctg ttgccaccaa tggcaacgac cacaagctct
    14941 ttcatgttgt tatgtccgct acagagggtg attaggttat gaccaacgcc agactgcctg
    15001 atgcgctacg cttatcaggc ctacaattct cgggccatcc gtaggccgga taaggcgttc
    15061 acaccgcatc cggcataaac atgtaagtca gacgccgtaa cgttcgcacc agccgagaat
    15121 ggctttctcg aagcaggcga atggtggatg cacaatgccc gcgccaatca tgccgacgcc
    15181 cgcatcttta tgggcgatgg cggtgttgat caccggcaga atgccgctgc tacccacgcg
    15241 ggtgatgtcg attgccgacg gcacgcccat aaaaccgagc agcggaatgg tgacgttggg
    15301 gttttcgcca agggtgattt cgcgcatctg acgggagaaa tcgatagcct cttccaccgt
    15361 gccgcccacc agtgcgacga ttgcaggcgc ggtcgccatg gcaaacccgc caataccgta
    15421 ggtttctgtg atggcgctgt cgccgatatc cagccccgaa tcttccggct tataaccagc
    15481 aaacatcggg ccgatgacct gctgcgctgg gccggtaaac cactgccccg gcaggccgct
    15541 gacccgcagg ccgaactcga cgccgttacg cgccatggtg gtgaccacgg tgctgtattc
    15601 gatgccgtgc gccgcatcca tcgccgcttt acacatcgcc atccacgtcg ggccggagaa
    15661 gtagtcgctg ctggcgacaa actcgaacac ttcgcgctgt tgctcgacgg aatagcctgc
    15721 ctgaatgatc cccggcgtca gtgcctgaat cagcagcgtc gtcccggcgt tattgcggtt
    15781 atggcactca tcgcccatat gcagcgcctg cgccagcatt aagcgcagat cgatctcacc
    15841 gataattttc atcgcgtcgc gcagcattgg cccctgcaca tcgcgcatcc agttgaggcg
    15901 gtcgatcacg ctctgatcgt tggcacccat acgcaaaatc ttcgccatct gctcgctcat
    15961 attggtataa gcgatgttgc cgtaggtttt gtttttcacg atgtgcataa acatcgaggc
    16021 cgaggtaacg cccgccatcg atcccacgca gtcgtgttcg tggcacggcg agaaggtgat
    16081 ctccccggag gccgccagtt cagtcgcctc atcgagatct ttcgccagcc cttcgaacac
    16141 caacgccccg gtgaccgcgc ctttcatcgc gccgcacatt ttttcccagg tgatcggcgg
    16201 cccggcgtga agaatggttt tcggcgtcat gcccggcacc acgttaatcg cctgatcaaa
    16261 accaatcagc accggatgcg actggataat gcgctccagc gcctgctggt tagcggcggc
    16321 gattttgtct gccagcggcg aatcggcaat gttatccagc gcctgcacca cctgcatatt
    16381 gccctgcccc ggcggcgtcc agtcgagttg agtcacttct acatgctgct ttttcaggtc
    16441 atcgctaaac atggcgatac cgacgttaat aacgttcaac ggttggctaa acagtgactg
    16501 gctcatcagg cttcctcccc tttgcagata aattcacgcg ccagcaatcc ggtattggtg
    16561 ctgctgctcg ccagaatcac tccggcatca agcagcatct ggctttgttg ttctaatgat
    16621 ggcgtatcga gatcggtgcc cagcacatag gcgagaatga tcaactcgcg tccttcggcg
    16681 gcggcgatcg ctttcgcttc tttgatcgcc tcgatggtgg agccgaccgg atcttcatgc
    16741 gatccaaatc cgagcacaaa atccatcacg atcaccgcca cttctggatc gcgcgcttct
    16801 tcgatcaagc gactgatgcg gttggtgggg tcaatcattg gatgcggctt gccgttggtg
    16861 aagtcgtcat cgccaaagtc gaggaaggtg tgtttgatgc tgcggttgat atcttgcagg
    16921 cggaattcag gatcgggctg aatgttgctg tagacatcgc catgtttttc catcaccgcg
    16981 aacatggttt cgtcgcacag cgtgccgccg cagaacaggc cacgaatgta tttctgctgc
    17041 ggttgcagac gcgcacgcac atcagcaatc aacggctggt taagcgtatg caggtcgaga
    17101 ttttcctgtt tcacgccgga gagcatcacc gcttttagcg ccgcctcttt gctgccgcgg
    17161 gcaaactgta gcccctgctc atccaccggc gtttcaacgc gaccgaggaa gcagaccacc
    17221 accggcttgc ggcaggcacg tgcgcgttcc agcactttgc gggccaccgc aggcgcggga
    17281 ggttttgaaa tcagtgcgat aatttcagtt tgcggatcgt tttccagcat cccgatggcg
    17341 tcgagcatca tcaggccgcc gattttctcg ctcaggtcgc gcccgccggt gccaatcagt
    17401 tgtgaaacac cgccaccaaa ttcatgaatg cggacgctca gctcctggct gccggtgccg
    17461 gatgcgccaa caataccgat attgccgcga cgcacggcgt taccaaaaca gagcgccgcg
    17521 ccgttaataa tcgccgtgcc gcagtccggc cccatcatca gcagcccttt ttcgtgggcc
    17581 aactgcttga gcgccagttc atcttcaact gagacgttat cggaaaacag catcacgtta
    17641 aggtcgtttt gcagcgcctg acgcgcttcg cgagcggcaa acagaccgtt aaccgaaatc
    17701 accgccaggt tactttccgg gatatgcttt ttggcgctgg caatagtggc gtaacgcgcc
    17761 tcgtgcgagc cgctttgcgc tttgctgttg aacagttctt caatctccac cagcaactgt
    17821 tcgttgtccg cgcccgcttt gccgttgatg acaatcatca ggtcgccgtt tttcgcctgt
    17881 tccagctccg gcgtcagcag tcctaaattt ttcagtacac ctttgttcat ttcggtcgcc
    17941 atcgccacaa atgcctgctc gacgccgtcg agtttatttg cacgcgtgga gatagacatc
    18001 agcgagacag aatcaaaata ggtattcggt ttaatgacta ttttaactga cataattccc
    18061 tccgtaggtt tggctcagcg cacaaccatc ggccatgcca tacagcatgt cgcagccgga
    18121 actggagcca atatgtttaa ttttttctat tgcctgagtt gcgttcccag ggatgtcata
    18181 aataatgttg tgaataaaac gatgaatatt ttcccggcaa cgttgttgta atgcggcttc
    18241 cagcgttata gcacttaata atgtggtgtt atttctgccg cgctgcatac cgagataaaa
    18301 ttcctctttg tatttttccg acggatgccc gggaataaat aaaataagcg ccagacctgt
    18361 taaataatcg tcggctgatg gcgtaaggcc aatccctaac ccaatcatac tggcgaccgc
    18421 tgcggcgata ttttgttttt cgtttactgc ctgaataact gcccgtcgtt taatatgtag
    18481 ttctttattt aacgcctgat aaaacggatt atcgccgcga taattaaaca gggtttcatt
    18541 ttcccccagg tgctgatgaa taatgtcgtg ccactgtagc caggcgataa ggtgaaaatt
    18601 cacagcggtc atttgcaagg ttggggacag ccaacgccga caacgactca tctctatatg
    18661 aagatgttga ccaaccgtaa taccttgatc gtcaaactga accttatcac catgccggaa
    18721 cagatcatca aagtgagtga gtgccaaccg acaactgttt ggcgcattgt cgtactcttc
    18781 acaaagcagc gtcagcaact gctggcgagc cgggagtgcg atatttaccg ctctggcaaa
    18841 gacctgttca acctgtcctt gcccccgctg cgccagaaaa tccgcatccg cagttagcgc
    18901 ccacacacac cgtctttctg gtgtgttgat agcaagcctc ccggtttatg cacctgcggc
    18961 aatcagtaac tgcgcaatct cttcaaagcc ccgttcccgc gccagttcca gtggcgtttt
    19021 gccatattta tcggtcagat gcgggctggc accgtgttcc agcaataact gcacaatcgc
    19081 ctgctgttta ataccaccat cattaagcac aatcgcttcc agtagcggcg tccagcctac
    19141 atggttggtc tggttaacgt taatctccgt atgcgccaaa agctctttta caatacttaa
    19201 atggcctttt tcacaggcag gcgtcaggcc gacaccgcca aaacgggtta cgcaattaag
    19261 atcgggttta gccggtaaaa taattcgtag tagagttaaa tcatcgttca gacagctaat
    19321 taaaaaagga tttaaacagg tatgatcttg cttattaata tccgctccgg catcaattaa
    19381 tgcctgaacg caagcatatt gctgatataa acttgccagc gtaattgccg ttttcccctg
    19441 acgatcgcag gtattaatat cgacacccag cgcaaggcag gtttttactt tatcgatatc
    19501 accctgttgt gcagccaata aatagtcggc aggtagattt tttatactca ttcacattcc
    19561 cctggttttt gtggctgttt tcgatgaatt gagaatagca acgataatct gataaaagaa
    19621 cccgcttaaa tttaattcgc tgtatttcgc ctttttgttg aatgatgttc aacaatcttt
    19681 tcgcagtgtg cggtagtgca atatttgttt ctttgacccg ataacctttc actcaatcag
    19741 gttttatttc tctatagtct gcacatgtaa gagtagcttt cgtccatgtc taaccagagg
    19801 ggaacaagat gaacggactc acggcaacgg gtgtcaccgt cggtatttgt gcagggcttt
    19861 ggcagttagt gtcttcacat gtcgggctct ctcagggatg ggaattatta ggaaccattg
    19921 gcttcgtcgc cttttgcagt ttttatgccg caggcggcgg taaatcgggg tttatcagaa
    19981 gtctggcggt taattattcc gggatggtgt gggcattttt cgccgcgctg gctgccggtt
    20041 ggctggcctc tgtaagtgga ctgtccgcgt tctgggcgag cgtaattact accgtaccct
    20101 tctcggcggt agtggtctgg caagggcgtt tctggttact gtcctttatt cccggtggct
    20161 ttctcggcat gacgctgttt tttgccagcg ggatgaactg gacggtgacg ttactgggtt
    20221 ttctggcggg taactgcgtg gggataattt cagaatatgg tggacaaaaa ttaagcgaag
    20281 cgacgactaa acgcgacggg tattaaaagt tattcaaaaa tgattatcta tctttaaccg
    20341 atacctctac agtcatagct ggagaaattc atagtagata atggcgagaa tggtttaatg
    20401 aactcaattt ttaccgaaga gaatctgttg gcatttacta ccgctgcgcg ttttggcagc
    20461 ttcagtaaag ccgcagaaga gttgggttta accacttccg ccattagcta caccattaag
    20521 cgtatggaga cggggctgga tgtggtgctg ttcactcgca gtacccgcag cattgagtta
    20581 acggagtccg ggcgttattt tttccgcaaa gctaccgacc tgctgaatga tttttatgcc
    20641 atcaaacgta gcatagatac tatttcgcag ggcatagaag cgcgtgtgcg catttgtatt
    20701 aatcagctgc tttatacgcc caaacacacc gcgcgcttat tacaggtgct gaaaaagcag
    20761 tttcctacct gccagattac cgtcaccacc gaagtgtata acggcgtctg ggatgcgatt
    20821 attaataatc aggccaatat cgccatcggc gcacccgata cactactgga cggcggcggt
    20881 attgattaca ctgaaattgg cgcgattcgc tgggcttttg ctatcgcccc ggatcacccg
    20941 ttagcctttg tgccggaacc catcgccgaa agccaactgc gtctctaccc taatattatg
    21001 gtggaggaca ccgcgcatac gattaataaa aaagtgggct ggttgctgca tgggcaggag
    21061 tcgattctgg tgccagattt caacaccaaa tgtcagtgcc agattttagg tgaagggatt
    21121 ggttttttgc cggattacat ggtccgtgag gcgatggcgc aatccctgct ggttacccga
    21181 caaatccata atccgcgcca ggattcgcgc atgttgctgg cgactcagca ttctgctacg
    21241 ggtcaggtga cgcagtggat taaaaaacaa tttgcgccaa atggcatatt aacggggatc
    21301 tatcaggatt tactgcatcg ggagaattaa tcgctgccaa cctcccgatg cgctgtgaat
    21361 agcggtttac atcaaccacc tgctttcatt acccattcag agataaattt attacccggt
    21421 actggcggag agtacaaata gccctgcaaa aagtgaacgc ctttaccgat cattaaatcc
    21481 gcctgctcct gggtttctac cccttccgcc acgatactca gaccaggctt acgcgctagt
    21541 tcgacaatat tgtccacaat atgaccggag atttcgtcga cactcgccat ttgcacaaat
    21601 gacttatcga tcttaataaa atcgaccggg aacgcctgca agtaacgata ggtcgcataa
    21661 cccgtaccaa agtcatccag cgcaaaggta atgttgtgct gatgaaggct gtcaaatatc
    21721 gctctggctt ctggcgttac cggaataggg ttacgttccg ttagctcgag aaccagcttg
    21781 attttatcgt tacctaattt cttaaccagg ttcagacact ctttttcaaa tcccgctgcc
    21841 aaaaaacaac ccgccgagac gttgatgcca atatggaaat tgtccggcag caaatgtttt
    21901 accggcatca gaatatccgc agtctgtttc atcagttggc gggtcattat gacaataaga
    21961 ccggatgact ccgccagagg aataaactga tccggtggga taattcccgt ttgtggatgt
    22021 tcccagcgga caagtacctc acagcccgtc agtaccccag tctgcgcgca gaaaaccggt
    22081 tggatccacg gtttgaattc gtggttttcc aacgccagac tgatggcttc aggcgtgacg
    22141 atatggtgat agtgattatc attaatatat ctatgactat tactacccgt ggcggatatg
    22201 atctccggat tgtactgaaa gaaaatatcg cgccagaaag taatatcgct ctgaatcccc
    22261 agtttctcaa agagctgttt cttttgatgt gaaattgtct ttgcactgcg atttctgtac
    22321 tgtgatattt ctgttacaga cattccctgc atcagcagct tgagtagcag tcgttctttc
    22381 tcggatacgc taccaggcaa tgacaaatga accgttgtac cgaactcttg cagaaaaaca
    22441 cgaaaatcac atgaattcat gaacacacct ttatctttta tcgctgttca gtatgagtga
    22501 ttacctgtgg attcctgagc acacataagg ggtaaccata aacattagcg atgccatacg
    22561 tataacatcc cacatcacta tcaaaaagat tatctttacc tctgatgctc gctcaaaaac
    22621 agttacgcta aataccttaa aaaggtaatc acaagacaaa ctcaggagat aatcacgcaa
    22681 gaatgcaata ttaaaattca gatactcatt tttggtaata aagaagtagt tggactattt
    22741 caatatttat aatttcccac tacgtactgg catccattca tgagcaaagg cgcattatat
    22801 gaatttaaca atccagatca actgaaaata cctctccctc ataaacacat agcgtcaaca
    22861 ttcaatgaca taatgagtaa agatgttggt tatgcatacg tatcattact ctatgcctgt
    22921 cccttaaaaa cccacccatt aagactgaat ccattcagca aatgataacc aatattatag
    22981 caatgacaat aaaaacaata tttaaaacaa tcaataaaat atcagacaca gacaattaaa
    23041 ccttaaacaa caaacggtta cattgatttt agttgatttg cgctacgcgt tccccatgaa
    23101 taaagcgtca tttcaaatta gcaccaatat ttacattaaa taagactaag cttattgaac
    23161 attcacaaca gtccaaacgt aggttttggg ctgcaatagt tcttttcttt tttattatct
    23221 gttgcctggc aatcatcaat cgatactaag ggcttaaccg tacagatata acgtatcaag
    23281 aggtaagaga aaccgcgcca tatgcagaca tggcgcggtt ttatgcaata acaatcacgc
    23341 gtccgggaac atcacgctat ggcccggcgc ttcacgatgg agatgaataa agttaaggtg
    23401 ccgctcgtac tggtcaagaa tatcggtgat cacctgctct ttgctgtagt ccatcaggtc
    23461 gttgccctgg ctgccttcta acaggaaggt ttccagccgg tagtaggtcg atttaccgct
    23521 acgtgcgcgg taggtaaagc ccggcaccga gtattgctgc ggccaaatct gatagacaaa
    23581 gttttgttct tcgcccatat gcaccaacaa atccagatgc cccaactgtt gtccctcttc
    23641 cggcggcagg ctttttagct ccacgtatgc gccgcgcaac cgcagctcct gcgccacttc
    23701 ttccattgcc gggtaacaga ccgtctccat catctgttta gtgtaacgcg tgcccggata
    23761 attcatcagg cgcgagagac gttttttcca gctcaggcga tcctgaagcc ccagcggtcg
    23821 cggtgcggta tcgcggttgg cactttcacg gcggtaatct tctaccttca gagatttata
    23881 caaccccgcc atcacgaaga agatcacaaa gctgaacggc agccccataa tcaccgtggt
    23941 gttttgcagc gcggatatcc cgttggtcat cagcatgccg agcgtcagca ggccaatcgc
    24001 caccgaccag aagacgcgca gccagccggg ggcgtcgctg ttgatatctt taagctgcga
    24061 ggtgaaattc cccagcacca gcgccccgga gtccgccgag gtcacataaa acagcaggcc
    24121 agtaatggtg gcgacggagg cgctaaaggt aaacgccgga tactgcgcca gcaggctgta
    24181 gaagccgcgc tccggatgga ccatcgcttc ctcggcaaat gccgctccgc cgtggatgat
    24241 ttcatacagc gcgctattgc cgaacaccga gagccataac agcgtgaagg taaacggaat
    24301 aatcaacgtg cccagcacga actggcgaat ggtacgccca cgcgagatac gcgccaggaa
    24361 caagccgaca aacggcgacc atgccaccca ccatgcccag aagaagagcg tccagttatt
    24421 catccactca actggacggt cgaaggcaaa actgttgagc gtcatgccca taaagcgatt
    24481 cacatagtcg ccaacattca gcaccagcgc attaagcagg aacgaggtgt cgcccataaa
    24541 caatacgaac aggatcaatc ccagcgccag cgcaacgtta agttccgata acacacgaat
    24601 ccccttatcg acaccggagg tgacagagat cgtggcgatt atcaccgaca aggcgatcag
    24661 tgccgctttt gccgccatcg aatcgggaat atcaaacagt acgctcaagc catagttaag
    24721 ctgcaccaca ccgataccga gcgtagtggc aataccgaag atggtgccga tcaccgctgc
    24781 aatatccact gagtgcccta tcggcccgtt aatccgttta ccgaagatcg ggtacagcgc
    24841 cgagcggatg gtgagcggca aattataacg atagctaaag tatccgagcg ccatgcccat
    24901 cagcgcatac atcgaccagc cggttaagcc gtagtgaaac agcgtccaga ccatcgcctg
    24961 acgcgcggcc tcaatcgtct gtcccgcgcc ttccggcggc tgcatatact gcgttaccgg
    25021 ttcggctacg gagaagaaca tcaggtcgat accgatcccg gcagcaaaca gcatcgccgc
    25081 ccaactcagc aggctgaatt ccggtttgga ttgttctggc ccgagcttca ccgaaccaaa
    25141 acgcgaacaa gcgatacaga ccacaaagac aatatagagc gttgccgcca gtagatagta
    25201 ccaaccgaag gttttagaaa cccagtccag cgtgcggcca atccacaggg ccgagaagtc
    25261 gcgaaacagg atcgttgtca gggaaaacaa caaaatcagt ccggcggagg tgtaaaacac
    25321 caccggattg attttgtcct tttccctgct gtgtgaaagg tctgtcatcc agtatcccca
    25381 ctgttattgt taccatttaa aatcaaattc gtaacaatta agacacattt tatattgaac
    25441 gtccaatcaa taaccgcttt aatagataaa caacgctgat gaatggagtg gcgaaaatgc
    25501 ccaaattggg gatgcagtcg atccggcgca gacaactgat cgacgccaca ctggaagcaa
    25561 taaatgaagt gggcatgcac gatgcaacga tcgcgcagat cgcccgccgt gcaggcgttt
    25621 ctacggggat catcagccac tatttcaggg acaaaaatgg tctgctggaa gcaaccatgc
    25681 gcgatatcac cagtcagctg cgtgacgcgg ttttgaatcg attacatgca cttccgcagg
    25741 gcagtgcaga gcagcgatta caggcgattg ttggcggaaa cttcgatgaa acgcaggtga
    25801 gcagtgcggc gatgaaagcc tggctggcgt tctgggccag cagtatgcat cagccgatgc
    25861 tctatcgttt acagcaggtc agcagccgcc gcttgctgtc gaatctggtg agcgagtttc
    25921 gccgcgaatt gccgcgcgaa caggcacagg aagcgggcta cggccttgcc gcgctgattg
    25981 acggattatg gctgcgcgcg gcactgagcg gcaaaccgct ggataaacct ctcgcacact
    26041 cgctgacccg tcactttatt actcagcatt tacccaccga ttaaccgagg agacgtgatg
    26101 tcccgaatgg cagaacagca gctttatata catggtggtt atacctccgc caccagcggt
    26161 cgcaccttcg agaccattaa cccggccaac ggtaacgtgc tggcgaccgt gcaggccgcc
    26221 gggcgcgagg atgtcgatcg cgccgtgaag agtgcccaac aggggcaaaa aatctgggcg
    26281 gcgatgaccg ctatggaacg ctcgcgtatt ctgcgtcggg ccgttgatat tctgcgtgaa
    26341 cgcaatgacg aactcgcaaa acttgaaacc ctcgacaccg gaaaagcata ttcggaaacc
    26401 tcaaccgtcg atatcgttac cggtgcggac gtgctggagt actacgccgg gctgatcccg
    26461 gctctggaag gcagccagat cccgttgcgt gagacgttat ttgtttatac ccgccgcgaa
    26521 cctctgggtg tggtggcggg gattggcgca tggaactacc cgattcagat tgccctgtgg
    26581 aaatccgccc cggcgctggc ggcaggcaac gcaatgattt tcaaaccgag cgaagtcacc
    26641 ccgcttaccg cgttaaagct ggctgaaatt tacagcgaag cgggcctgcc ggacggcgta
    26701 tttaacgtgt tgccgggggt gggcgcggag accgggcaat atctgaccga gcatccgggc
    26761 attgccaaag tgtcatttac cggcggtgtc gccagcggca aaaaagtgat ggctaactcg
    26821 gcggcctctt ccctgaaaga agtgaccatg gaactgggcg gtaaatcacc gctgatcgtt
    26881 ttcgatgatg cggatctcga tctcgccgcc gatatcgcca tgatggcaaa cttcttcagc
    26941 tccggtcagg tgtgtaccaa tggcacccgc gtcttcgttc cggcgaaatg caaagccgca
    27001 tttgagcaga aaattctggc gcgcgttgag cgcattcgcg cgggcgacgt tttcgatccg
    27061 caaactaact tcggcccgct ggtcagcttc ccgcatcgcg ataacgtgct gcgctatatc
    27121 gccaaaggca aagaggaagg cgcgcgcgta ctgtgcggcg gcgatgtact gaaaggcgat
    27181 ggcttcgata acggcgcatg ggttgcaccg accgtgttca ccgattgccg cgacgaaatg
    27241 accatcgtgc gcgaagagat cttcggtccg gtgatgtcgc ttctcactta tgaatctgag
    27301 gacgaagtga ttcgccgcgc caatgatacc gactacggcc tggcggcggg catcgtgacg
    27361 gcggacctga accgcgcgca tcgcgtcatt catcagctgg aagcgggtat ttgctggatc
    27421 aacacctggg gcgaatcccc ggcagagatg cccgttggcg gctacaaaca ctccggcatt
    27481 ggtcgcgaga acggcgtgat gacgctccag agttacaccc aggtgaagtc catccaggtt
    27541 gagatggcta aattccagtc catattctaa ccaggaggtt tatttgcaat ttgactacat
    27601 cattattggt gccggctcag ccggcaacgt tctcgctacc cgtctgactg aagatccgaa
    27661 tacctccgtg ctgctgcttg aagcgggcgg cccggactat cgctttgact tccgcaccca
    27721 gatgcccgca gcgctggcgt tcccactaca gggcaaacgc tacaactggg cctatgaaac
    27781 agagcctgaa ccgtttatga ataaccgccg catggagtgc ggacgcggta aaggcctggg
    27841 tggatcgtcg ctgatcaacg gcatgtgcta catccgtggc aatgcgctgg atctcgataa
    27901 ctgggcgcaa gaacccggtc tggagaactg gagttatctc gactgcctgc cctactaccg
    27961 caaggccgag actcgcgatg tgggcgagaa cgactaccac ggtggcgatg gcccggtgag
    28021 cgtcaccacc tccaaacccg gcgtcaatcc gctgtttgaa gcgatgattg aagcgggcgt
    28081 gcaggcaggc tacccgcgca cggacgatct caacggctat cagcaggaag gttttggccc
    28141 gatggatcgc accgtcacgc cgcagggccg tcgcgccagt accgcgcgcg gttatctcga
    28201 tcaggccaaa tcgcgcccta acctgaccat tcgtactcac gctatgaccg atcacatcat
    28261 ttttgacggc aaacgcgcgg tgggcgtgga atggctggaa ggcgacagca ccatcccaac
    28321 ccgcgcaacg gccaacaaag aagtgctgtt atgtgcaggc gcgattgcct caccgcagat
    28381 cctgcaacgc tccggcgtcg gcaacgctga actgctggcg gagtttgata ttccgctggt
    28441 gcatgaatta cccggcgtcg gcgaaaatct tcaggatcat ctggagatgt atctgcaata
    28501 tgagtgcaaa gaaccggttt ccctctaccc tgccctgcag tggtggaacc agccgaaaat
    28561 cggtgcggag tggctgtttg gcggcactgg cgttggtgcc agcaaccact ttgaagcagg
    28621 cggatttatt cgcagccgag aggaatttgc gtggccgaat attcagtacc atttcctgcc
    28681 agtagcgatt aactataacg gctcgaatgc ggtgaaggag cacggtttcc agtgccacgt
    28741 cggctcaatg cgctcgccaa gccgtgggca tgtgcggatt aaatcccgcg acccgcacca
    28801 gcatccggca attctgttta actacatgtc acacgagcag gactggcagg agttccgcga
    28861 cgcaattcgc atcacccggg agatcatgca tcaaccggcg ctggatcagt atcgtggccg
    28921 cgaaatcagc cccggcacgg aatgtcagac ggatgaacag ctcgatgagt tcgtgcgtaa
    28981 ccacgccgaa accgccttcc atccgtgcgg tacctgcaaa atgggttacg acgagatgtc
    29041 cgtggttgac ggcgaaggcc gcgtacacgg gttagaaggc ctgcgtgtgg tggatgcgtc
    29101 gattatgccg cagattatca ccgggaattt gaacgccacg acaattatga ttggcgagaa
    29161 aatagcggat atgattcgtg gacaggaagc gctgccgagg agcacggcgg gatattttgt
    29221 ggcaaatggg atgccggtga gagcgaaaaa atgagtcgtg atttgaactg actcaagaac
    29281 cgatctgatt gagagttacc gttccaggta tggggacaga atcgttatat aaaacatagg
    29341 ggtaactctc atgcttcata gtgacaacct cgccagcaaa ttctaagcgg actcgttcaa
    29401 tctggctgat gaaggtgcta tgaatgcact gataaaccgc aggtgaaatg ccactcagct
    29461 taagaattac gccgctagtg ttcctggcta tggtgacttg ttcactcccg ctcacggtta
    29521 gcatatacga aaagggttct tgaaacgagg acaaaaataa gtcagaccaa aacaacgtgg
    29581 agctttaaac acgattcgta taatttattg gcagtgattt atttttactc ttctttaatt
    29641 aatcatcatg cttaaatatt ttttagcaca agattatcgc ttcgctaaca ttttatttta
    29701 cattaatttt caattgataa taaaaagttg ttaataagtt tatgcgaaaa ataaaaattg
    29761 ttgaaatact ctccagcaaa tgaatcaaaa ataaacaaat tgatatttac aaaaagcaaa
    29821 aaactattta agagaacatc tttaatttat tagcagaacc catcaataat gatttactta
    29881 cgactaaaaa atatcttaca acaatgtgtt attgtaaatt aacgggaata atattaaatt
    29941 tttattttct acaccctaac ttcacagaac gacaaaatgc atataaaatc aataagataa
    30001 ataataaaaa caacctcata agaaataacg tcaaatatct caaaactact aaacatcact
    30061 aaaatattag acgtaacaca catcccccac gccctgtagt tcatagcttt tatatttatt
    30121 ttctcacatc actgtagata aaaacacgca ttaaaaatga cttgtagaaa agctgcacaa
    30181 taaatagttc atatttaatc tgttgcgtag aactactatt caaaaaatct taataatgag
    30241 ataatgagat tacatcgata acgttctgat tgcaggcata cttatctggg tgtgcctctc
    30301 ttttttttaa aactgtacac ggtggcgtat gacgagaaaa tatctcacac aggatgaagt
    30361 ctacaggctg atggatgcag cccagagtat gtcctttcct gaaagaaatc gctgtctaat
    30421 tatgatggct ttcattcacg gttttagggc cagtgaactt cttgatttac gtttatccga
    30481 tattgatgca tctggaaaac aacttaatat tcggcgtatt aaaaatggtt tctcgacaac
    30541 acatcctctt cttcctgatg aatataattt gatcaagttg tggcttaagc agaggaagtt
    30601 aattgaaaac ggagttgaag gagactggct ttttctctcc cggaaacgcc gccctatcag
    30661 cagacaacat tttttttcta tcattcgtga ggctggaaaa cgtgcaggat tagctgtaaa
    30721 agcacatcct catatgttac gtcatgcctg tggttttgct ctggctgaca atggtgtcga
    30781 tacccgacta cttcaggatt atctgggtca tcgaaatatt cagcatactg tcagatacac
    30841 agccagtaat gctgctcgtt ttaaaggggt gtggaaaaaa aagcctcgtt aaaacagtac
    30901 cttctgattc aaaatgtcag attcctacgt tttcctcaat tcttccataa aaaaacagcc
    30961 cattcaatta aataaaacac ataacatgtt gatatttctc gctaaagtaa aataacccca
    31021 tgatttgaaa tgattaacca caaagatcat accagtgatt aactttcgct catattgtta
    31081 aataacacaa aattaaaaac aataaaaatc aaacaaatta gatttatatt ccaagaaaat
    31141 taaatattca ggctattaaa ccaaacctga cattttgaat caaaatgtca ggttttgtag
    31201 caatggcgtg gacgagtctg aatgaagaag cctctggtta ttatcagtgc ctgtcagttc
    31261 accaggctgg cactggagtc tctcatccca gcggacaggt atatcgtgcg ggtgtacagc
    31321 aacgtcacta cagaggttga attcgtttta aagacctcct gtggctacct gttggctgat
    31381 atcccttcgc ttccttcggg ggagttagtt cttctggctt gtcttgtccg cttgcggagt
    31441 gatgccgggc aatggcaagt ctgcctgacc ggtgactccg actggttcga taatgctcct
    31501 gcttcaagtc tgtataacgg cttcccacat atcggcacct tgctgaaagc gagtcgttat
    31561 cagaatcaaa ttatccgctg gttactacgt cctttacccg gcgatatgag tgatttctgg
    31621 ctcctgactg cacgggagtt aagcgtgctt aagattctga tgcagggcat gagtttcagt
    31681 gagattgccc gttatgaaca acgcagttca aagactctgc atgcgattgc cacacaggca
    31741 ttaaggaaac ttggactggg aacactaagc gatttcaggc tgctctatac aggatgcggt
    31801 gacagcagag tcaaacggaa tatacggcta catgcaagat atgtcggaca acattccgct
    31861 catcgactca ctcagtatgt gcagaacatg gtgtccggaa ttctttatcc agagaaagtt
    31921 atcgttggta aatcaataaa ttctgacgcc atatccggaa aagtgctttc cgggcaaaga
    31981 aaacatatgt ctaaaaaaag accaggagtg ttatgacaca gcagcgttgt tactaccacc
    32041 cactgcggcg cgtacggact cgctacagtg taggggccaa aatatttcta ctgctctggg
    32101 ctgcaggagt gggaggcacg ctgtacagcc tgtacagcgt gctggcgtac tgacatctgt
    32161 gagggaagga tgcgtttcgt tcttttctgt ccctcaggaa ttgtacctgc acagtttgaa
    32221 gctctatcaa ccggttctgt ctgcaatatt acctgcatca gaacagaaga agagttacgc
    32281 aataaattgc ggcacaggcc gcagagtgtc gtgatatcgg cagggcgacc tgccgaatgc
    32341 gctgaaatgt ggttccgatt ttatcgggat cactcattcg tggtggtgtt gtgcgtcgcg
    32401 cccttttttc tgccaccgga tgtgagtatt tctggcgttc tcaaaaacct gaggttactg
    32461 aaaacaggaa tgtcagtaga gcgtgctatc agtgttgcaa atgcctctgg tgggttttca
    32521 ggactgaaac atgcagaaaa tttgccagtt atggacagtt actctgtctt tatgaatgag
    32581 gtgaataacc gcacaaagac aatcgtcatg tcagaacgct tcccggaaaa gcaaaagaag
    32641 gtgttgtcgt tgcttcttgc tggacacagc tgggaataca gtgcgcagtt tctcaaaacg
    32701 gggataagac agatatggct tgctgagcag tcgctaaaaa agcgttgggg aataccggac
    32761 agcatgtcat tgcgggaagc cctgctgctg agcagcaata aatttcacgg tgatggaaat
    32821 gcattggaaa aaacgaatgc catgacattg cgtgaaaacg gaaataccaa caggtattcc
    32881 gtggtggtaa atgcagggac gcaggcttta tctctgcata aatataaata agtgggaaat
    32941 tgaaattagt taccagaatg gagaatttct tcatgaaaaa cagtaaggca ttttaccgca
    33001 gcgcattagc gacagctatt gttatggctc tttctgcacc agcattcgct gctgataacg
    33061 cggtatcaac tacaccggtt acactggata aagataagac aactctggat caagatgttg
    33121 ttattagcaa tacagcagac caacagatta cagccgtaac aattaatgcg gcagataaag
    33181 atcttaatgt tacttttggc ggtcatgata ttactgctga atcaacggca gacaaaaaat
    33241 tccttgaagg tgtaaaagtt agcggtgaca aaaatgttgt gattaatgct acaggctcca
    33301 ccatcacagc tcaaggtgaa ggcacctatg tccggactgc aatggtcatt tcttcaactg
    33361 gcgatgttgt tgttaatggc ggtaatttcg ttgcaaaaaa tgaaaaaagt agtgcgacag
    33421 gaatatctct ggaaggggcc acgggaaata atgtaacgct aaatggtaca accataaatg
    33481 ctcaaggtaa taagagttcc agcaacggct ctacggcaat ttttgctcaa aagggtagcg
    33541 tattgaatgg ttttaaaggt gatgcaaccg acaacattac ccttgctggc tcaaatatta
    33601 ttaatggccg aattgaaaca atagttattg ccaaggagaa tacgggaact catacagtca
    33661 atctgaatat taaggatggc tcagtaattg gggcggctaa taataaacaa acaatttatg
    33721 cttctgcttc ggcacaagga gcaggttcag caacgcaaaa tttaaattta tctgtcgccg
    33781 attcaaccat ctactctgat atccatgccc tttctgcaag cgagaattca gccggtacca
    33841 caacaaatgt aaacatgaac gttgcccgct cttactggga aggtaatgct tataccttca
    33901 atagcggcga taaagcgggt agtaatctgg atataaatct ttccgatagc tcagtctgga
    33961 aaggcaaagt ttcaggggca ggagatgcca gtgtatctct gcaaaacggg tctgtctgga
    34021 atgttacggg atcctcaact gttgatgctc tggcagtaaa agacagtacg gttaatatca
    34081 cgaaggctac agtcaatact ggcacgtttg cttctcagaa cggcactctg attgttgatg
    34141 cctcttctga aaacactctg gatatcagcg gaaaagcgag cggtgacttg cgtgtttaca
    34201 gtgcgggttc attggatctt atcaatgaac aaacggcatt tatttctacc ggcaaagaca
    34261 gcactctaaa agccacaggc acaacggaag gtggtctgta tcaatatgac ctgacacagg
    34321 gagctgatgg taacttttat ttcgtaaaaa acacgcataa agcatccaac gccagctccc
    34381 tgattcaggc aatggcagca gctccggcta acgttgctaa tctgcaggct gacacgctct
    34441 cagcccgtca ggatgctgtc cgtctgagcg aaaatgacaa gggcggcgta tggattcagt
    34501 actttggcgg taaacagaaa cataccaccg cgggaaatgc atcctatgac ctggatgtaa
    34561 acggtgtaat gctgggtggt gatacccgct tcatgactga agatggtagc tggctggcag
    34621 gtgtagcgat gtcttctgcg aaaggtgaca tgaccaccat gcagagcaaa ggcgacactg
    34681 aaggttacag cttccacgct tacctgagcc gccagtataa caacggtatc ttcattgata
    34741 ctgctgcaca gtttggtcac tacagcaaca cggcagatgt tcgcctgatg aatggtggcg
    34801 gtaccatcaa agctgacttt aacaccaatg gtttcggtgc gatggttaaa ggcggttaca
    34861 catggaaaga cggtaatggc ctgtttattc agccatatgc caaactgtct gcgctgacgc
    34921 tggaaggtgt ggattatcag ctcaacggcg tggacgttca ttctgacagc tataactctg
    34981 tgctgggtga ggccggtacg cgcgtgggtt atgacttcgc tgtgggcaac tcgaccgtta
    35041 aaccttatct gaatctggcc gcactgaacg aattctctga tggcaacaaa gtccgtctgg
    35101 gtgatgagtc tgtcaatgcc agcattgacg gtgcagcatt ccgcgtgggt gcaggtgtac
    35161 aggctgatat caccaaaaac atgggagcat atgcaagcct tgactacacc aaaggtgacg
    35221 acattgagaa cccgctacag ggtgtagttg gtatcaatgt gacctggtaa gaataaagca
    35281 gtcggttccc ctgtcgactg ttataccccc tgcaggcagc atactgctgc ctgcaggcta
    35341 taaacatcca gtattcatga tgctggtttt ttgctgatat ttaccccgaa cattcctgat
    35401 acaggctatt aatacaacct tatctgcggc agattttatt ttctggatat attatgcgtg
    35461 aacaaatcaa acaggatatc gatctgattg agattttatt ttatctgaag aaaaagattc
    35521 gtgttatcct ttttattatg gctatatgta tggctatggt gctgttgttt ctgtatatca
    35581 ataaagacaa tataaaagtg atttacagcc taaaaataaa ccagacaacg ccaggtatac
    35641 ttgttagctg tgatagcaat aataattttg cctgtcagac cacaatgact gaagatgtta
    35701 ttcagcgaat tactacattt tttcacacca gcccagatgt caagaacaga gaaataaagc
    35761 tggaatggtc aggagataag agagatttac caactgctga agcggaaata tctcgcgtgc
    35821 aggcgtctat tatcaaatgg tatgcgtcag aataccataa tggcaggcaa gttctcgatg
    35881 agatacaaac accttcagca attaacagtg agctttatac aaaaatgata tacctgacca
    35941 ggaactggtc attgtatccg aacggtgatg gctgtgtaac tataagctca ccagaaataa
    36001 aaaataaata ccctgctgcc atttgcctgg ctctgggatt ttttctaagc attgtaattt
    36061 ctgtaatgtt ttgccttgtc aaaaaaatgg tagatgaata ccaacaaaac tctgggtagt
    36121 aataaacagc attctgatag agagtgcctg tataaagctg aagtgacaat gaccgggaag
    36181 ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg taaatatcct ggcattacta
    36241 atgccaggat atttacagaa gagcagcata accatgacgg aacggggtta agtcaggttg
    36301 tccttaacgc tattttcaac ctcgtatgcc ttcttcaggt ttatgttcag atctcatatc
    36361 tctctcagca atcctcaata atcagataca ccgctttcac cggaccatga accccgacga
    36421 ctttgataag ctcaatatcc gccgttgaac tagggccgct aatgatgtta atgcaggacg
    36481 gcattcgttc accggcctgc gctttctgat gcaatttttc tgcgagttgc gctacacgcg
    36541 gcaggattgt gcttttacgc aggataaaaa gagaagattc cgggagcagg ctcaatgaac
    36601 gcccgcgccc ggcggcggaa aaaagaacca cgcctcccga ttcggttaaa ccatattcag
    36661 catacacgac acccacttta gcctgctcgg cctgcgagat attctcggca cctttcgccg
    36721 gatcccaaac aacggcattg cattcctgct gcaaacgttc actaatcccc aattcctcga
    36781 gcctcgtgtc accgctaatc acgaccgact gatctcccag ctctttacac agacgtattg
    36841 cagcttctgc cgccttcgcc tcgctggtca gctcacagcg cgtcaacata acatcgctgg
    36901 caaactgaat aaacgcgtca cagcgctgct gttggttaag ttgggtaagc cgctcgttag
    36961 catagttgtt aagcggcgca tcttctgctt gcggttcaag tcgcagcggg cgacccagtg
    37021 cctgagcaac gttattcaaa aattcgcctc gattatccat tctttttctc ctgcgcctga
    37081 tgtttcttaa accaactacg gaaactctct ccgtcagctt caggaagatc gcgtgcttcc
    37141 atccagtcgc taatcgcgcc aaatttgagt ggtgttttgc cgccattgat aaaccagctt
    37201 gccgcatgag cgccggccat catcccgact ttccacaatc ctggatgact attggcataa
    37261 gcgaacattt ttatcgcccg ttgctctgct tttgcggtga tccctttttc agccatcacc
    37321 cgacgatgac tcaaaatcag ttttgacagc ggaatacgca ccggacacac gctgtcacaa
    37381 gcagtgcata aagagcaggc gtagggtaaa tctttaaaat ctttatagcc gccaagtagc
    37441 ggagaaatca ccgcaccaat tggccctgga taaatagagc catatccatg accgccaata
    37501 tggcgatatg ccggacaagt attcatacaa gccccgcagc gaatacagcg cagcacatcc
    37561 cgaaattcag aggccagcac ctcagaacgc ccgttatcga caataaccag atgaaactct
    37621 tcaggaccat caacgtgccc cgcttcgcgc ggtcctgtca gccaggtgtt gtatcccgtc
    37681 aaacgtgcac caacggcact gcgcgccagc atggtgatca atacatctac ctcggcaaac
    37741 gtgggggcaa tacgctccat tcccatcact gcaatatgcg ttttaggcag cgtggtacac
    37801 attcgcgcat taccttcatt ggtcaccagg cataccgaac ctgtctctgc caccgcgaaa
    37861 ttacagccgg taatacctat ttcagcactg aggaaatctt cgcggatttt ttgccggatg
    37921 aataaggtca ttgcttcagg cgtttccggc ccctcatagc ccagacgttc gtgtagcact
    37981 cgacggatct gatggcgatc tttatgaatt gccgggacca caacatgaga tggcggatct
    38041 tgatccagct ggagaatata ttcacccaga tcggtttcaa tcacctgaat gccagcatcc
    38101 tgcaatacat gattgacacc aatctcttcg gtcaccatcg atttagattt caccaccttc
    38161 cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg tagcgtcttc tttggttttt
    38221 gcaaaccaga cgtgaccgcc gttttgcgtc actttttctg agagctggta cagataagcg
    38281 tcgagattac tcagaacatg atcacgtatc tgggcagccc gatcgcgcca ctcctcccag
    38341 tgccccaatt catcgaccat tttttgccga tttgccccaa tacgctgctg cgcgtttgcc
    38401 accgctttgc gcatgatcgg atcttcaatt tgctgacgga tgcgtgtctt aaaatctgta
    38461 ttactggttt tgatcgacat ctttatatcc tcagcggctc atcaacactt cagcaatatg
    38521 catcactttg actttctgcc cttcccgttg taatcgccca ctgatgttca gcaggcaact
    38581 cacgtcagca ccaattaaat actcagggcg gacttccatc aggtgcgcaa ccttttcttt
    38641 caccatctcg ccggatattt cggccatttt gaccgagaac gtgccgccaa atccgcagca
    38701 ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc acatttttca gcagcgtaag
    38761 tggttcgtcc ttcaccccca gcttacgggc caggctacaa gatgggtgat acaccgctct
    38821 cccttgcaaa ctggcaccta catcgactac ccctaattta ttaacaataa aagaggtgag
    38881 atcctgcata cgcgcggcaa ccttttcggc tcgtgatgcc cattcaggtt catccgccag
    38941 atacgtcggg taacttttta cggcataggt gcaagagcca gctggtgaaa taatgggatc
    39001 gtcgttatcc tccagtgcgg cgatcagatt tttcatccct ggaatcgctt ctttgatata
    39061 accgctattg atcgcaggct gaccgcagca tccctgtttc tccgggaaat ttacgcgaca
    39121 gccgagtttt tccagtagca gcacggagtc tcgtgccatt cttgatttca gggcgtcacc
    39181 aatacaggtg acaaagaaat tgacattcac aactaatact ccattacttc atgcccattt
    39241 atgcccgatg tttccccccc accgggggat caatcatcaa taaatatggc gttctttttt
    39301 aattggctag caacatacca cccctcattt gtatgacaaa tgataatctt tcatccaaac
    39361 ttcaaatgca ataactaatt ttatttcttt cggcatcgct cacattttat ggggaaatcg
    39421 acgatcattt ttggcataag aaatccattt aaaaatggcc acatccctta tacctattca
    39481 gagctactta ttcaggggtt attgagatat acactgatgg tgtacaaaca aaatcaccga
    39541 caataatcac gtatcaagta atagtcggag ttttatttaa ttgaagcaat ttagtttgca
    39601 tttttattta aaaaccgagc tagagtccat cagaaaaaaa atatataaag cccaaaccac
    39661 cgttggaaat agaaatagct gaaaggctca caacacccgg aatgcggatt atctttgctc
    39721 cgattcctgg cggttttatt cagggtgcaa gctgtctgac cctttcccga tattctcccg
    39781 gggtacaacc aaactcgcgg acaaacgcct tgtgaaaaga tgattcactg gcatagccta
    39841 ctgactcagc gatcaccaca acagggagcg tttcccggga aaacatctgg gccgctattt
    39901 gtagacgcaa ctttgttaat acagccagcg gcgtggttcc ggaaacatca cggaaaagct
    39961 gggcaaaact tgcccgggac atgtgggcga tgctggccag cgattcgacg gtccaggcgt
    40021 gtcctggcat ttccaacatt tgctgtatta ccgcaccaag acgtggatgc agaagcaaac
    40081 tgagaatgtt tttctcagta ttaacctgtg caatccactc acgcaccgcg agggtaaaga
    40141 atgtagcgca gatctggcta cacagtgcat ccactcccgg cattgccaat ctggattcct
    40201 gttgtaaaaa cgggatcgcc tccctcagcc agttatattc tacgctgtgg ttaaccggtg
    40261 ctaaaaacag cgtttccggc aaagacgtta aaaaataacg cgccgaatgt tgcaaccgaa
    40321 gagtgccgca gacaatacag gtcgattcat tatcgacatg actcaggcga tgagctgaat
    40381 tttgtggtag caggaccaca tttcccgggc gtaatgtaaa aatctctccc gtcggcattt
    40441 ccagcttcgc cgctccttgc gttaacgcat gccaacgaat aaccgataat tccccggcac
    40501 catgtggaag ctgccagtca cttcctaaca cgcaattctt atcgatcgtt ccttgtggag
    40561 cgttaagcat caacaaacgg ctaagggcat ccatatcaga ctcctgagtg gtttttgatt
    40621 acaaacacca acaatcttag taaataacta atctaatacc atcaaatagt taatcatgat
    40681 ttttgttttg tgattctgat taagaaaaag aaaataccag acgataagac aaaatatcaa
    40741 gcgttgtgaa gaaatggtat ttgctctttt gtgtctatag tcatgatgtc aaatgaacgc
    40801 gtttcgacag gaaatcatca tgaataaata tcaggcagtg attattggtt ttggcaaggc
    40861 tggaaaaaca ttagccgtca cgctggcaaa agcaggttgg cgagtggctc tcatcgaaca
    40921 atcaaatgca atgtatggcg ggacctgtat taatatcggc tgcatcccaa ccaaaacatt
    40981 ggttcatgac gcacagcagc acacagattt tgtccgtgcc atacagcgta aaaatgaagt
    41041 ggttaatttt ttacgtaata agaattttca taatcttg
//
LOCUS       DAEVZA010000029        39723 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEVZA010000029 DAEVZA010000000
VERSION     DAEVZA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.029"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..542
                     /locus_tag="LAF92_001588"
     CDS             <1..542
                     /locus_tag="LAF92_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBI9790613.1"
                     /translation="DVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLTLAILDGMSVRV
                     ADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWKVFNVAGAKTEA
                     TQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAATILGDGSVALIV
                     DVSALQAINREQRMANTAA"
     gene            563..1066
                     /gene="cheW"
                     /locus_tag="LAF92_001589"
     CDS             563..1066
                     /gene="cheW"
                     /locus_tag="LAF92_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBI9790614.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            1211..2872
                     /gene="tar"
                     /locus_tag="LAF92_001590"
     CDS             1211..2872
                     /gene="tar"
                     /locus_tag="LAF92_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBI9790615.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            2918..4519
                     /gene="tap"
                     /locus_tag="LAF92_001591"
     CDS             2918..4519
                     /gene="tap"
                     /locus_tag="LAF92_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBI9790616.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            4538..5398
                     /gene="cheR"
                     /locus_tag="LAF92_001592"
     CDS             4538..5398
                     /gene="cheR"
                     /locus_tag="LAF92_001592"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBI9790617.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            5401..6450
                     /gene="cheB"
                     /locus_tag="LAF92_001593"
     CDS             5401..6450
                     /gene="cheB"
                     /locus_tag="LAF92_001593"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBI9790618.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            6465..6854
                     /gene="cheY"
                     /locus_tag="LAF92_001594"
     CDS             6465..6854
                     /gene="cheY"
                     /locus_tag="LAF92_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBI9790619.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            6865..7509
                     /gene="cheZ"
                     /locus_tag="LAF92_001595"
     CDS             6865..7509
                     /gene="cheZ"
                     /locus_tag="LAF92_001595"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBI9790620.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            7711..8859
                     /gene="flhB"
                     /locus_tag="LAF92_001596"
     CDS             7711..8859
                     /gene="flhB"
                     /locus_tag="LAF92_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBI9790621.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            8852..10930
                     /gene="flhA"
                     /locus_tag="LAF92_001597"
     CDS             8852..10930
                     /gene="flhA"
                     /locus_tag="LAF92_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBI9790622.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGIVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            10930..11322
                     /gene="flhe"
                     /locus_tag="LAF92_001598"
     CDS             10930..11322
                     /gene="flhe"
                     /locus_tag="LAF92_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBI9790623.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(11442..11930)
                     /locus_tag="LAF92_001599"
     CDS             complement(11442..11930)
                     /locus_tag="LAF92_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBI9790624.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(12107..13840)
                     /gene="argS"
                     /locus_tag="LAF92_001600"
     CDS             complement(12107..13840)
                     /gene="argS"
                     /locus_tag="LAF92_001600"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBI9790625.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            14056..14622
                     /locus_tag="LAF92_001601"
     CDS             14056..14622
                     /locus_tag="LAF92_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI9790626.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            14636..15382
                     /gene="cutC"
                     /locus_tag="LAF92_001602"
     CDS             14636..15382
                     /gene="cutC"
                     /locus_tag="LAF92_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBI9790627.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            15770..16870
                     /locus_tag="LAF92_001603"
     CDS             15770..16870
                     /locus_tag="LAF92_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBI9790628.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGSGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            16895..19324
                     /gene="torZ"
                     /locus_tag="LAF92_001604"
     CDS             16895..19324
                     /gene="torZ"
                     /locus_tag="LAF92_001604"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBI9790629.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRSSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAGLRKKYAVADREPITIHTEDATRFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(19489..20460)
                     /gene="cmoB"
                     /locus_tag="LAF92_001605"
     CDS             complement(19489..20460)
                     /gene="cmoB"
                     /locus_tag="LAF92_001605"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBI9790630.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLIPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(20457..21200)
                     /gene="cmoA"
                     /locus_tag="LAF92_001606"
     CDS             complement(20457..21200)
                     /gene="cmoA"
                     /locus_tag="LAF92_001606"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBI9790631.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(21241..21636)
                     /locus_tag="LAF92_001607"
     CDS             complement(21241..21636)
                     /locus_tag="LAF92_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBI9790632.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(21689..22507)
                     /locus_tag="LAF92_001608"
     CDS             complement(21689..22507)
                     /locus_tag="LAF92_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBI9790633.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            complement(22504..23070)
                     /locus_tag="LAF92_001609"
     CDS             complement(22504..23070)
                     /locus_tag="LAF92_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI9790634.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            23380..25152
                     /gene="aspS"
                     /locus_tag="LAF92_001610"
     CDS             23380..25152
                     /gene="aspS"
                     /locus_tag="LAF92_001610"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBI9790635.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            25270..25722
                     /gene="nudB"
                     /locus_tag="LAF92_001611"
     CDS             25270..25722
                     /gene="nudB"
                     /locus_tag="LAF92_001611"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBI9790636.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            25751..26491
                     /locus_tag="LAF92_001612"
     CDS             25751..26491
                     /locus_tag="LAF92_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI9790637.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEQMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            26526..27047
                     /gene="ruvC"
                     /locus_tag="LAF92_001613"
     CDS             26526..27047
                     /gene="ruvC"
                     /locus_tag="LAF92_001613"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBI9790638.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(27049..27651)
                     /locus_tag="LAF92_001614"
     CDS             complement(27049..27651)
                     /locus_tag="LAF92_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBI9790639.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            27722..27787
                     /gene="yobI"
                     /locus_tag="LAF92_001615"
     CDS             27722..27787
                     /gene="yobI"
                     /locus_tag="LAF92_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBI9790640.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            27926..28537
                     /gene="ruvA"
                     /locus_tag="LAF92_001616"
     CDS             27926..28537
                     /gene="ruvA"
                     /locus_tag="LAF92_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBI9790641.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            28546..29556
                     /gene="ruvB"
                     /locus_tag="LAF92_001617"
     CDS             28546..29556
                     /gene="ruvB"
                     /locus_tag="LAF92_001617"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBI9790642.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(29703..30488)
                     /gene="znuB"
                     /locus_tag="LAF92_001618"
     CDS             complement(29703..30488)
                     /gene="znuB"
                     /locus_tag="LAF92_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBI9790643.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(30485..31240)
                     /gene="znuC"
                     /locus_tag="LAF92_001619"
     CDS             complement(30485..31240)
                     /gene="znuC"
                     /locus_tag="LAF92_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBI9790644.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            31319..32251
                     /gene="znuA"
                     /locus_tag="LAF92_001620"
     CDS             31319..32251
                     /gene="znuA"
                     /locus_tag="LAF92_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBI9790645.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            32267..33589
                     /gene="mepM"
                     /locus_tag="LAF92_001621"
     CDS             32267..33589
                     /gene="mepM"
                     /locus_tag="LAF92_001621"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBI9790646.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            33709..34680
                     /gene="lpxM"
                     /locus_tag="LAF92_001622"
                     /gene_synonym="msbB"
     CDS             33709..34680
                     /gene="lpxM"
                     /locus_tag="LAF92_001622"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBI9790647.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(34769..37843)
                     /locus_tag="LAF92_001623"
     CDS             complement(34769..37843)
                     /locus_tag="LAF92_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBI9790648.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     CNQLLKSLYYDVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTDEIIRILEYIDN
                     TLITFCNEEIIKCQIAIQSIISKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YALMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTHADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNRIMENKLHDAEFFITITNNMTSILRSEFDEIWKAML
                     TLQFHDILPGSCISRVYHETEKEYLKLEAKTEKIISDAQSTLLSKIDTSSYKDPHILF
                     NTTCFARNEWININNNWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSE
                     SGDLISLYDKRYGKEYITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMT
                     TVISGPKTTMTLIYHYNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQ
                     TSLAQCQIQFGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQETGIALIAKNKYGYRVK
                     DQTLELTLLRSQHKPGEVVKADNYDNFLINNFADIGKQKFTFSLFPHHGDYRVGGVVN
                     QAYIMNHPLQIVPVKPHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESYG
                     LEISAMLSWVCNYHYVQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
     gene            complement(37880..39688)
                     /locus_tag="LAF92_001624"
     CDS             complement(37880..39688)
                     /locus_tag="LAF92_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HBI9790649.1"
                     /translation="MNGHIGELDKLVPHLITEHKIYGRDRLTPRTVSAEDGVIKKQNN
                     PDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEKASDLCLELIDTQDDDGYIGI
                     YDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESLLTSLTQAAQCIMAGYPKETS
                     HPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYCLWLYEDYSAGCVSEQDLQYK
                     NVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALDIFLSQLKYYLTPSGAPIGDE
                     WITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGLADDAEWLFYNASFGMQHPIL
                     HAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEAAVCCVPNTARTIPAFVENMF
                     QENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDLSVKCLIEPESSVRFALSFRY
                     PGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDIKFIADIQFNTDLCGDTYISR
                     GPVLYAIELESDILIKKTLLNGKYFDSGYIPCSRADESIQFSYADMESFSYSTSPEGK
                     QYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
BASE COUNT        10221 a         9423 c        10320 g         9759 t
ORIGIN      
        1 tggatgtcgt taaacgtaat atccaggaga tgggcggtca tgtcgaaatc cagtcgaagc
       61 agggtactgg cactacgatc cgcattttac tgccgctgac gttggccatc ctcgacggca
      121 tgtccgtacg cgttgcggat gaagttttca ttctgccgct gaatgctgtt atggaatcac
      181 tgcaaccccg tgaagccgat ctccatccac tggccggcgg cgagcgggtg ctggaagtgc
      241 ggggtgaata tctgcccatc gtcgaactgt ggaaagtgtt caacgtcgcg ggcgcgaaaa
      301 ccgaagccac ccagggaatt gttgtgatct tacaaagtgg cggtcgccgc tacgccttgc
      361 tggtggatca attaattggt cagcaccagg ttgtggttaa aaacctcgaa agtaactatc
      421 gcaaagtccc cggcatttct gctgcgacca ttcttggcga cggcagcgtg gcactgattg
      481 ttgatgtctc cgccttgcag gcgataaacc gcgaacaacg tatggcgaac accgccgcct
      541 gaatgagtaa aaaggtaaca atatgaccgg tatgacgaat gtaacaaagc tggccagcga
      601 gccgtcaggc caggaatttc tggtatttac ccttggtgat gaagagtacg gtattgatat
      661 cctgaaagtg caggagatcc gtggctacga tcaggtaaca cggattgcga acacgccagc
      721 gtttatcaaa ggcgtcacga atctgcgcgg ggttattgtg ccgattgttg acttacgaat
      781 taagttcagc caggtggatg tggactataa cgacaacacg gtagttatcg tcctgaatct
      841 cggacaacgg gtggttggca tcgtggttga cggcgtttca gacgtgcttt cattgacggc
      901 ggagcaaatt cgtccggcac cagaatttgc cgtgacgctt tcaacagaat atctcactgg
      961 actgggcgca ctgggcgacc gtatgttgat tctggtgaac atcgaaaaac tgctgaacag
     1021 cgaagagatg gcgctgttag atagcgcggc gtcagaagtg gcgtaattct cccgatttcc
     1081 tcaattgaaa tgaacccgat gatctgcgca tcgggttttt tatttcaatt tcgcggcggg
     1141 tggcatcagc aataaagttt ccccccttct tgccgataac gagatcaact tgttttcagg
     1201 aaggtgcctt atgattaacc gtatccgcgt agtcacgctg ttggtaatgg tgctgggggt
     1261 attcgcactg ttacagctta tttccggcag tctgtttttt tcttcccttc accatagcca
     1321 gaagagcttt gtggtttcca atcaattacg ggaacagcag ggcgagctga cgtcaacctg
     1381 ggatttaatg ctgcaaacgc gcattaacct gagtcgttca gcggtacgga tgatgatgga
     1441 ttcctccaat caacaaagta acgccaaagt tgaattgctc gatagcgcca ggaaaacatt
     1501 ggcgcaggca gcgacgcatt ataaaaaatt caaaagcatg gcaccgttac ctgaaatggt
     1561 cgctaccagt cgtaatattg atgaaaaata taaaaactat tacacagcgt taactgaact
     1621 gattgattat ctagattatg gcaatactgg agcttatttc gctcagccaa cccagggaat
     1681 gcaaaatgca atgggcgaag cgtttgctca gtacgccctc agcagtgaaa aactgtatcg
     1741 cgatatcgtc actgacaacg cagatgatta ccgatttgcc cagtggcaac tggcggttat
     1801 cgcgctggtg gtggtattga ttctgctggt ggcgtggtac ggcattcgcc gtatgttgct
     1861 tactccgctg gcaaaaatta ttgctcacat tcgcgaaatc gccggtggta acctggcgaa
     1921 taccctgacc attgacgggc gcagtgaaat gggcgacctg gcgcagagcg tttcacatat
     1981 gcaacgctct ttgactgaca ccgtcactca tgtccgcgaa ggttcagatg ccatctatgc
     2041 cggtacccgt gaaattgcgg cgggcaacac cgatctttcc tcccgtactg aacagcaggc
     2101 atccgcgctg gaagaaactg ccgccagcat ggagcagctc accgcgacag tgaagcaaaa
     2161 cgccgataac gcccgccagg cctcgcaact ggcgcaaagt gcctccgaca ccgcccagca
     2221 cggcggcaaa gtggtggatg gcgtagtgaa aacgatgcat gagatcgccg atagttcgaa
     2281 gaaaattgcc gacattatca gcgttatcga cggtattgcc ttccagacta atatcctcgc
     2341 gctgaatgcc gcggttgaag ccgcgcgtgc gggtgaacag ggccgtggtt ttgccgtggt
     2401 ggcgggtgaa gtgcgtaatc ttgccagtcg cagcgcccag gcggcaaaag agatcaaagc
     2461 cctcattgaa gactccgtct cacgcgttga taccggttcg gtgctggtcg aaagcgccgg
     2521 ggaaacaatg aacaatatcg tcaatgctgt cactcgcgtg actgacatta tgggcgagat
     2581 tgcatcggca tcggatgaac agagccgtgg catcgatcaa gtcgcattgg cggtttcgga
     2641 aatggatcgc gtcacgcaac agaacgcatc gctggtgcag gaatcagctg ccgccgccgc
     2701 tgcgctggaa gaacaggcga gtcgtttaac gcaagcggtt tccgcgttcc gtctggcagc
     2761 cagcccactc accaataaac cgcaaacacc atcccgtcct gccagtgagc aaccaccggc
     2821 acagccacga ctgcgaattg ctgaacaaga tccaaactgg gaaacatttt gacgagtatt
     2881 tactaacgcg gtcattgccg cctgatgggg agcgttgatg tttaatcgta ttcgaatttc
     2941 gaccacgctg tttttaattt tgattctctg cgggatcttg cagattggca gtaacggcat
     3001 gtctttttgg gcatttcgcg acgatttgca acgactgaat caggtcgagc agagcaatca
     3061 gcaacgtgcg gcattagcgc aaactcgggc ggtaatgtta caggccagta ccgcgctgaa
     3121 caaagcgggc actctgacgg cacttagcta tccggcggat gacattaaaa cgttgatgac
     3181 gacggcgcgc gccagtctga cgcaatccac cacgctgttt aaaagtttta tggcgatgac
     3241 tgcgggcaac gagcacgtca gggcgttgca aaaagagacg gagaaaagtt ttgcccgctg
     3301 gcacaacgat ctcgaacatc aggcgacctg gcttgaaagt aatcaacttt cggatttcct
     3361 cactgcgccg gtgcaggaat cacagaatgc gtttgacgtt aactttgagg cctggcagct
     3421 ggagatcaac catgtgctgg aagccgccag tgcgcaaagc cagcgtaact atcagatttc
     3481 ggcgctggtg tttatcagca tgattattgt tgcggcgatc tacatcagca gtgcgctgtg
     3541 gtggacgcgc aagatgattg ttcaaccact ggccattatc ggtagccatt ttgacagcat
     3601 tgctgcgggt aatctggcgc gtccgattgc ggtatatggt cgtaatgaga tcaccgccat
     3661 ttttgccagc ctgaagacca tgcagcaggc tttgcgtggg acggtgagtg atgtgcgtaa
     3721 gggaagccag gagatgcaca ttggtatcgc ggagattgtc gcaggcaata acgatctctc
     3781 aagtcgtacc gaacagcagg cggcatcgct ggcacaaacg gccgccagta tggagcaatt
     3841 aaccgccacg gtagggcaaa acgccgataa cgcacgacag gcgtcggaac tggcaaaaaa
     3901 tgccgcgaca acggcgcagg cgggcggtgt tcaggtcagt accatgactc acaccatgca
     3961 ggagatcgcc accagctcgc aaaaaattgg cgacattatc agcgttatcg acggaattgc
     4021 tttccagacc aatattctgg ccctgaatgc ggcagtggaa gcggctcgcg ccggagagca
     4081 ggggcgtggt tttgcggtag tggcaggtga agtgcgcaat cttgccagcc gtagcgcgca
     4141 ggcagcaaaa gagatcaaag ggctgatcga agagtcagtc aatcgtgtcc agcagggttc
     4201 gaaactggtg aataacgccg ccgcgaccat gatcgatatt gtcagttcgg tgacccgcgt
     4261 gaacgacatt atgggagaaa ttgcctctgc gtcggaagaa caacagcggg ggattgagca
     4321 ggttgcacag gctgtcagcc agatggatca ggtgactcag cagaacgcct cgctggtaga
     4381 agaagcggcg gtggcaacgg aacaactggc gaatcaggcc gaccatcttt cgtcgcgtgt
     4441 ggcggtattt acccttgaag aacatgaagt agcacgacat gagtcggcgc agttacaaat
     4501 tgcgccagtg gtatcctgaa gtgattgaga aggcgctatg acttcatctc tgccctgtgg
     4561 gcaaacgtct ttattgttac agatgaccga gcgcctggcg ctttccgacg cgcattttcg
     4621 gcggataagt caattgatct atcaacgagc cgggatcgtt ctggctgacc ataaacgcga
     4681 catggtttac aaccgactgg ttcgtcgttt gcgttcgctg ggactgacgg atttcggtca
     4741 ttatctgaac ttgctggaat ctaatcagca cagcggtgag tggcaggcgt ttatcaattc
     4801 gctgaccacg aatctgacgg catttttccg tgaggcacac catttccctc tgctcgcgga
     4861 tcacgcacgt cgccgttctg gcgagtatcg cgtatggagc gcggcggctt cgaccggcga
     4921 agagccgtac agcattgcga tgacgctggc tgacacattg ggcaccgcgc ccggacgctg
     4981 gaaagtgttt gccagtgata tcgacaccga agtgctggaa aaagccagaa gcggtattta
     5041 tcgccatgaa gagttgaaaa acctgacgcc gcagcaactg caacggtatt tcatgcgagg
     5101 gacgggaccg catgaagggc tggtacgcgt gcgtcaggag ctggcgaact atgttgattt
     5161 tgccccgctg aatctactgg cgaaacagta caccgtgccg gggccgtttg atgcgatctt
     5221 ctgtcgtaac gtcatgatct acttcgatca aaatacccag caggagattt tgcgccgctt
     5281 tgttccgctc cttaaacccg acggattgct gtttgcgggt cactctgaaa actttagcca
     5341 ccttgagcgc cgcttcacgc tgcgtggtca gacggtgtat gcgctaagta aggattaacg
     5401 atgagcaaaa tcagggtgtt atctgtcgat gattcggcac tgatgcgcca gatcatgaca
     5461 gaaatcatca acagccatag cgacatggaa atggtggcga ccgcgcctga tccgctggtc
     5521 gcgcgtgact tgattaagaa attcaatccc gatgtgctga cgctggatgt tgaaatgccg
     5581 cggatggacg gactggattt cctcgaaaaa ttaatgcgtt tgcgtccaat gcccgttgtg
     5641 atggtttctt ccttgaccgg caaaggatca gaagtcacgc tgcgcgcgct ggagctgggg
     5701 gcaatagatt ttgtcaccaa accgcaactg ggtattcgcg aaggaatgct ggcgtatagc
     5761 gaaatgattg ctgaaaaggt gcgtacagca gcaaaggcga gccttgcagc acataagcca
     5821 ttgtcggcac cgacaacgct gaaggcaggg ccgttgttga gttctgaaaa actgattgcg
     5881 attggtgctt caacgggtgg aactgaggca attcgtcacg tactgcaacc gttgccgctt
     5941 tccagcccgg cactgttaat tacccagcat atgccgcctg gtttcacccg ctcttttgcc
     6001 gacagactta ataagctttg tcagatcggg gttaaagaag ccgaagacgg agaacgtgtt
     6061 ttgccgggac atgcctatat cgcgccgggc gatcggcata tggagctgtc gcgtagtggc
     6121 gcaaattacc aaatcaaaat tcacgatggc ccggcggtta accgtcatcg gccttcggta
     6181 gatgtgttgt tccattctgt cgccaaacag gcggggcgta atgcggttgg ggtgatcctg
     6241 accggtatgg gcaacgacgg cgcggcggga atgttggcga tgcgtcaggc gggggcatgg
     6301 acccttgcgc aaaacgaagc aagttgcgtg gtgttcggca tgccgcgcga ggccatcaat
     6361 atgggtggtg tctgcgaagt ggtcgatctt agccaggtaa gccagcaaat gttggcaaaa
     6421 attagtgccg gacaggcgat acgtatttaa atcaggagtg tgaaatggcg gataaagaac
     6481 ttaaattttt ggttgtggat gacttttcca ccatgcgacg catagtgcgt aacctgctga
     6541 aagagctggg attcaataat gttgaggaag cggaagatgg cgtcgacgct ctcaataagt
     6601 tgcaggcagg cggttatgga tttgttatct ccgactggaa catgcccaat atggatggcc
     6661 tggaattgct gaaaacaatt cgtgcggatg gcgcgatgtc ggcattgcca gtgttaatgg
     6721 tgactgcaga agcgaagaaa gagaacatca ttgctgcggc gcaagcgggg gccagtggct
     6781 atgtggtgaa gccatttacc gccgcgacgc tggaggaaaa actcaacaaa atctttgaga
     6841 aactgggcat gtgaggatgc gactatgatg caaccatcaa tcaaacctgc tgacgagcat
     6901 tcagctggcg atatcattgc gcgcatcggc agcctgacgc gtatgctgcg cgacagtttg
     6961 cgggaactgg ggctggatca ggccattgcc gaagcggcgg aagccatccc cgatgcgcgc
     7021 gatcgtttgt actatgttgt gcagatgacc gcccaggctg cggagcgggc gctgaacagt
     7081 gttgaggcgt cacaaccgca tcaggatcaa atggagaaat cagcaaaagc gttaacccaa
     7141 cgttgggatg actggtttgc cgatccgatt gaccttgccg acgcccgtga actggtaaca
     7201 gatacacgac aatttctggc agatgtaccc gcgcatacca gctttactaa cgcgcaactg
     7261 ctggaaatca tgatggcgca ggattttcag gatctcaccg ggcaggtcat taagcggatg
     7321 atggatgtca ttcaggagat cgaacgccag ttgctgatgg tgctgttgga aaacatcccg
     7381 gaacaggagt cgcgtccaaa acgtgaaaac cagagtttgc ttaatggacc tcaggtcgat
     7441 accagcaaag ccggtgtggt agccagtcag gatcaggtgg acgatttgtt ggatagtctt
     7501 ggattttgat ttgtattgcc tgatgtggcg tgaccacgtc atatcaggcg gtctgataag
     7561 gcgatgacgc cgcatccgac aaacgcacac tatgcttgat gtgtggcagc aaaagcccta
     7621 aatcccgcct gttttgcccc ttactcaaac cattgaacgc tttgcgctct ggcatcattc
     7681 acgcttaata ctctttccag gattggcgac gtgtctgacg agagcgacga caaaacagaa
     7741 gcccccacac ctcaccgact agaaaaagcg cgggaagagg ggcaaatccc gcgttcccgt
     7801 gaactgacct cactgctgat tttgctggtg ggcgttagtg ttatctggtt tggcggtgtg
     7861 tcgctggccc gtcgattgtc gggcatgctc tccgctgggc tgcattttga tcacagtatt
     7921 atcaatgacc cgaatctgat cctcgggcag attattctgc tgatcagaga agccatgctg
     7981 gcgctgctgc cgctgattag cggcgtggtg ctggtggcgc tcatttctcc ggtcatgctg
     8041 ggagggctgg tatttagcgg caaatccttg cagccgaagt tttccaaact caacccgcta
     8101 ccgggcatta aacggatgtt ctcggctcag actggcgcgg agttgcttaa agcaattttg
     8161 aaaaccatcc tggttggcag cgtgacgggg ttttttctct ggcatcactg gccgcagatg
     8221 atgcgcttga tggccgagtc tccgattacc gccatgggta atgcgatgga tttggtaggg
     8281 ctatgcgcac tgctggtggt gcttggtgtc attccaatgg tgggatttga cgtctttttc
     8341 caaatattca gccacctgaa aaagctgcgt atgtcacggc aggatattcg tgatgagttc
     8401 aaacaaagcg aaggtgaccc tcatgttaaa gggcggatcc gtcagatgca gcgagctgct
     8461 gcacggcgtc ggatgatggc cgatgtgccg aaagcggatg tcattgtcaa taacccgacc
     8521 cactattcgg tagcgttgca gtatgacgaa aacaaaatga gcgcaccgaa agtggtcgct
     8581 aaaggtgcag ggctggtcgc gctgcgcatt cgtgaaattg gcgctgaaaa taacgtcccg
     8641 acgcttgaag cgccgccgct ggcgcgagcg ctgtatcgac atgcggagat tggtcaacaa
     8701 atcccgggtc aactgtacgc cgcggtggcg gaagtgctgg cctgggtctg gcaactgaaa
     8761 cgctggcgtc tggctggtgg acagcgccct gtacaaccta ctcatcttcc ggtgccggaa
     8821 gccctggatt ttattaacga gaaaccgacc catgagtaat ctggccgcga tgctgcgcct
     8881 gcccgcaaac ctgaaatcga cacaatggca gatccttgcc ggaccgattt tgatcctgtt
     8941 gatcttgtcg atgatggtgc tgccactgcc cgcattcata ctcgacctgt tgtttacctt
     9001 caatattgcc ttgtcgatca tggtgttgct ggtggcgatg tttacccagc gcacgcttga
     9061 gtttgctgcg tttccgacca ttctgttgtt tactacgttg ttgcgtctgg cacttaacgt
     9121 ggcttcaacc cgtatcattt tgatggaagg gcataccggc gcggcggcgg cagggaaggt
     9181 ggtcgaagcg ttcggtcact tcctcgttgg tggcaatttc gctataggta tcgtggtgtt
     9241 tgtcattctc gtgatcatca actttatggt cattaccaaa ggtgccgggc gtatcgcaga
     9301 agtgggtgcg cgctttgttc tcgatggtat gccgggtaag cagatggcga ttgacgccga
     9361 ccttaacgct ggattgattg gtgaagatga ggcgaaaaaa cgccgctccg aagtgactca
     9421 ggaagccgat ttttacggct caatggacgg ggcaagtaag tttgttcgcg gcgatgccat
     9481 cgccgggatc ctcatcatgg tcattaacgt tgtcggcggg ttgctggtcg gcgtgctgca
     9541 acatggcatg agcatgggac acgcggcgga aagttatacg ctattgacca ttggcgacgg
     9601 tctggtggca caaattccgg cgctggtgat ttctaccgcc gcgggggtca tcgttacgcg
     9661 tgtcagcacc gatcaggatg ttggcgagca gatggtgaat cagcttttca gtaacccaag
     9721 cgttatgttg ttaagcgccg ccgtgctcgg tttactcggc ctggtgcctg gaatgccgaa
     9781 cctggtattt ttgctgttca ctgccggatt gctggggctg gcctggtgga tacgcggacg
     9841 cgaacaaaaa gcgcctgccg aacccaaacc ggtaaaaatg gcagagaata ataccgttgt
     9901 cgaagcgacg tggaacgatg tacaactgga agattctctg ggaatggaag tgggttatcg
     9961 actgatcccg atggtcgatt tccagcagga tggtgagttg ttgggccgta tacgcagtat
    10021 ccgcaagaaa tttgcccagg agatgggatt tctgccgcca gtggtgcaca ttcgcgacaa
    10081 tatggatctg caacctgccc gctatcgcat tttgatgaaa ggcgtggaga ttggcagtgg
    10141 tgatgcttat ccggggcgct ggctggcgat taaccctgga accgctgccg ggacgttacc
    10201 tggtgaggcg accgtcgatc cggcatttgg cctgaatgct atctggattg aaagtgcgct
    10261 aaaagaacag gcgcagattc aggggtacac agtggttgag gccagcacgg tggtagcaac
    10321 gcatcttaac cacctcatta gccagcatgc cgcagagctg tttggtcgtc aggaggcgca
    10381 acagctgttg gatcgcgtcg cccaggagat gccaaagctg acggaagatc tcgttcctgg
    10441 catcgtcacg ctcaccacac tgcataaagt gctgcaaaat ctcctcgatg aaaaagtacc
    10501 gattcgcgat atgcgcacca ttctcgaaac gctggcggaa catgcgccca tccaaagcga
    10561 tccacatgaa ttaaccgccg tcgtgcgcgt ggcgttggga cgggcgatta cccagcagtg
    10621 gtttcctggc aaagatgaag tccatgttat tggcctcgat acaccgctgg aacgtttgtt
    10681 actacaggcg ctgcagggcg ggggaggact ggagccaggg ctggcggatc gtttactggc
    10741 gcaaactcag gaagcgctat cccgtcagga gatgctgggt gcgccgccag tattgttggt
    10801 gaaccacgcg ctgcgaccat tattgtctcg cttcctgcgc cgcagcttgc cgcagttagt
    10861 ggtcctgtcg aatctggaac tgtctgataa ccgacatatc cgcatgacgg cgacaattgg
    10921 cggcaaataa tgagaacctt attagcaata ttattgtttc cgctgctggt gcaagccgcc
    10981 ggggagggga tgtggcaggc aagtagtgta ggaattacgc taaatcatcg cggtgagtcg
    11041 atgtcgtctg cgcctctttc tacgcgacaa cctgcttcag gattgatgac gctggtagcg
    11101 tggcgttatc agcttatcgg cccgacacct tcaggactgc gggttcgttt gtgttcgcaa
    11161 tctcgttgtg tcgaattaga ggggcagagc ggaaccaccg tggccttttc cggtatagcg
    11221 gcagcagaac cgttgcgatt tatctgggaa gtgccaggcg gtgggcggtt aattccaccg
    11281 ctaaaggtac aacgtaatga agtgattgtg aattatcgct gaaggacaaa aaaattacgc
    11341 gctcgcgagt atgtttaata actcagtgaa aaaggctcga tgtcttgttg caattattta
    11401 tcaggaatag cttgcaacaa aacatgccaa aaagatgtga attatcctct tgtcaaaggg
    11461 cttggataaa aatcttcaga gttatggtat agctgcaact ggcgagttcg cgtattggtc
    11521 tgtatgatca tacatgagag ccattctgat tcagaataag ggtcacttaa atcctgaacg
    11581 tcctcatttg aaaggattaa ttcacattct ttgtccctaa aatccagcca ggctcgctgt
    11641 gattttttaa gcaagttaag ttgttcggta tcaggttttt ttacacctgt gcgtggcact
    11701 tgttctatat aacgataagc ttcaccatag gctttattta attgcgcatc gacgagattg
    11761 tattcttgtc gcaggcatga tttgatcacg ttaatgtcag tatcatcatc tgaatttttt
    11821 tgccaacaat ctttgatggc tgaaatgtca tggtcgttta ctataaccgt atcttctgcg
    11881 taagcctggc aagagataat gcctaatgtt aatacaagaa atttaaacac tgcggtattc
    11941 cttaaattca accgttatcg tttccctggg gccggatatt acgcttctgg cctcttatcc
    12001 aacagtcaga aaacaatggc atgtccattt tcagaataca tttagatggc aataaaaaag
    12061 cctgatcagg taaccctgat caggctttac tctcacgaaa aatcgattac atacgctcta
    12121 ctgtctcaat acccagcgta tccagaccca gctttagcgt cttcgctgtc agttgtgcca
    12181 gtttcagacg gctgttacgc acttcttcgt tttctgcgct gaggatcggg cagtgctcgt
    12241 agaagccaga gaacagacca gccagatcgt acaggtaagc acacatcaca tgcggcgtgc
    12301 cttcacgggc aaccacggtg agggtttctt caaactgcag caggcgagct gccagttgcg
    12361 cttcacgatc ttcacggatg attaccggag ctgcagccag ttgctcttcg ttaatttctg
    12421 ccttacggaa cacggacaat acacgcgtgt aagcatactg catgtatggc gcggtattac
    12481 cctcaaacgc cagcatgttg tcccagtcga agatgtagtc cgtggtgcgg tttttggaga
    12541 gatccgcata tttcaccgca ccaataccaa ccgcgttagc cagtttttcc agctcgtcag
    12601 ctggcatatc cgggttcttt tctgccacca gacggcgtgc acgttccagg gcttcatcca
    12661 acagatcggc cagtttcact gtaccacccg cgcgggtttt gaacggtttg ccgtctttac
    12721 ccagcatcat gccgaacatg tggtgttcca gcggtacgga ttccggtaca tagcctgctt
    12781 tacggacgat cgcccatgcc tgcatcaggt gttgatgctg acgggagtcg atgtaataca
    12841 gcacgcgatc ggcatgcagt gtttcataac gatatttcgc acaggcgata tcagtggtgg
    12901 tgtagagata gccgccatct ttcttctgaa tgatcacgcc catcggttcg ccttccttgt
    12961 ttttaaattc atcaaggaat acgacggtcg ccccttcgct ttctactgcc agacctttgg
    13021 ctttgagatc cgccacaatt cctggcagca ttgggttgta gaggctttcg cccatcacgt
    13081 catcacgggt cagcgtcacg ttgagacgat cgtaggtgat ctggttctgc gtcatggtga
    13141 tgtcgaccag tttgcgccac atctcgcgga aatattcgtc accgctttgc agttttacca
    13201 cgtagttacg tgcacgctcg gcaaactctt catcttcgtc gtaatgcttt ttcgcatcgc
    13261 ggtagaaacc ttcaaggtca gccagctcca tttcaccggc gttttcctgc tgctgctttt
    13321 ccagccatgc aatcagcata ccgaactgag tgccccagtc gccgacgtgg tttgcgcgaa
    13381 tcactttgtg accgaggaac tccagagtac gcactgctgc gtcaccaata atggtagagc
    13441 gcaggtgacc gacgtgcatc tctttcgcca cgtttggcgc agagtagtca accacaatgg
    13501 tctgtttttc tggcgtagca acaccgagac gatcggacgc cagcgcctgc tgaacatgtt
    13561 cagccaggaa tgccggatca aggaaaatgt tgataaagcc tggaccggcg atctcaactt
    13621 tgctggcgat accgttaaga tccagatgag tcagcacctg ctctgctaat tgtcgcggtg
    13681 ccatacccag ttttttagca actgccatca tgccgttagc ctgatagtcg ccgaactgaa
    13741 cttttgctga ctgacgaacc tgcggttcgc aatccgcagg cgcgcctgcc gcaatcatgg
    13801 cctgacggac tttttctgag agaagagcct gaatattcac cggaatacct tacattgatg
    13861 aagcgagcaa aaattgcccg caccagttaa agaaattagg gcgggagtat actgcaaatg
    13921 gctgttttcg tcagcaacgt gcgcgtggcg tattgccgtt ttcactattg catcagaagc
    13981 ggcgtgaaat cagcggttgg ttggcgcagg gcaacagggt aaattaacgc ttttactgaa
    14041 agacgaagag tgattatggc gaactggcaa tctatcgacg agctgcagga tattgcatcg
    14101 gatttaccgc gatttaccca cgcattagat gaactttccc tccgtctggg gttgaatatc
    14161 actccgctga ctgccgatca catttctttg cgttgccatc aaaacgccac tgctgaacgc
    14221 tggcgtcgcg ggtttgaaca gtgtggcgag cttttgtcag aaaatatgat caatggcaga
    14281 ccgatttgtc tgtttaaatt gcatgaaccg gtacaggttg cgcactggca gttttctatt
    14341 gtggagctac cgtggcccgg ggaaaaacgt tacccacacg aaggttggga acatattgaa
    14401 atcgttctgc cgggtgatcc agaaacgctg aatgcccgtg cgctggcatt gctttctgat
    14461 gaaggactca gcctaccggg gatctcagtt aaaaccagtt caccaaaagg tgagcatgag
    14521 cgattaccga atccgacgct ggcggtcacc gatggcaaaa caactattaa gtttcatccg
    14581 tggtccattg aagagatcgt tgccagcgag cagtcagcgt aattaaggag caaaaatggc
    14641 attactggaa atttgctgtt acagcatgga atgtgcacta acggcgcagc aaaacggcgc
    14701 agacagagtt gaattatgcg cagccccaaa agaggggggc ttaacgccgt cgttgggggt
    14761 actgaaatcc gtgcgccagc gggtgacgat ccctgtgcat ccgataattc gcccacgcgg
    14821 tggtgatttt tgctacagcg acggtgagtt tgccgccatt cttgaggatg tgcgcacggt
    14881 ccgcgaatta ggttttcctg gactggtgac gggcgttctc gatgttgacg ggaatgtcga
    14941 tatgccacga atggaaaaaa taatggctgc tgccggtccg ctggcagtga cttttcatcg
    15001 cgccttcgat atgtgcgcta accctttata tacactcaat aatcttgcgg aattgggcat
    15061 tgcccgggtg ctgacatcag ggcaaaaatc agacgcactg caaggtttat caaaaattat
    15121 ggaacttatt gcccatcgtg atgctccaat cattatggcc ggagcaggag tccgtgcaga
    15181 aaacctgcac cacttcctcg atgccggagt gctggaagtc catagctccg cgggagcgtg
    15241 gcaagcctca ccgatgcgtt atcgtaatca aggattgtcc atgtcatcag atgaacacgc
    15301 ggacgagtat tcgcgttata tcgtagacgg ggcggcggtt gctgaaatga aaggaatcat
    15361 tgaacgccat caggccaaat gatttttacc gttgcatcat gtcgcccaat atgatgcttg
    15421 ctcgtaccag gcccctgcaa tttcaacagg ggcctttttt tatccctgag cagtataaaa
    15481 aacgaacgat aaccgtgatc tgttgagcgg gtgacagtga acatagcgtt gtgctaataa
    15541 tattttttat attcacattg attatgggat ttaaattgct aaaactgata aatatatatt
    15601 ctaaatagca actgggttat tccttagcaa ttaatgatta cattgtaata aatcatattc
    15661 tttatcgatt atttcaggca gtgttgtgcc gagttatgca agcggttaat tcgttgtgta
    15721 tttaattata caatgatttc ggtgttctgg aatttattta gaggaatcta tgcgagggaa
    15781 aaaacgcatt gggttattgt ttttgctgat agcggttgtg gttggtggca gcgggttatt
    15841 gctggcgcaa aaagccttac ataaaacgtc ggatacagca ttttgccttt cctgccactc
    15901 gatgagtaaa ccttttgagg aatatcaggg aactgtccac ttttcgaacc agaaagggat
    15961 acgtgcggaa tgtgcagatt gccatattcc aaagtcaggg atggattatt tatttgctaa
    16021 attaaaagca tctaaagata tttatcatga atttgttagc ggcaaaatag acagtgacga
    16081 taagttcgaa actcatcgcc aggaaatggc cgaaacagta tggaaagaat taaaagcaac
    16141 tgactctgca acgtgccgta gttgccattc ttttgatgcc atggatattg cctcgcaaag
    16201 tgaatctgcg cagaaaatgc ataacaaagc acaaaagggc ggcgaaacct gtatcgattg
    16261 tcataaaggc attgcccatt ttccgccaga aataaaaatg gatgacaacg cggcgcatga
    16321 gctggaaagt caggccgcta cttcagtgac taatggcgca catatttatc ctttcaaaac
    16381 ttctcgcata ggcgagctgg ctaccgtgaa tcctggtacc gatctcaccg tcgttgatgc
    16441 cagtggcaaa cagccgatcg ttctgttgca gggttatcaa atgcagggca gtgaaaacac
    16501 gctctacctg gcggcaggtc aacggctggc gctagccaca ttaagtgaag aaggtatcaa
    16561 ggcgctcacg gtaaacgggg aatggcaggc tgacgaatac ggcaatcaat ggcgtcaggc
    16621 gtctttacag ggtgcgctta ccgatcccgc attagcggac cgtaaaccgc tatggcaata
    16681 cgctgaaaaa cttgacgata cctattgcgc tggttgccat gcgcctattg ccgctgacca
    16741 ttacaccgtc aatgcatggc cgtccattgc caaaggaatg ggtgcgcgaa ccagcatgag
    16801 cgaaaacgaa ctggacattt taacgcggta tttccagtac aacgccaaag atattaccga
    16861 gaaacagtga tcctcgttga tcagggagga agttatgaca ttaacaagac gtgaatttat
    16921 taaacacagc ggtattgccg ctggagcgct ggtggtgaca tcggcagcac cgctccccgc
    16981 atgggcagaa gagaagggcg gtaaaatcct caccgcggga cgttggggag cgatgaacgt
    17041 agaagtgaag gacggcaaga ttgtttcttc aacaggcgcg ctggcgaaaa ccataccgaa
    17101 ttccttacag tctacggcgg cggatcaggt acacaccacg acgcgtattc agcatccgat
    17161 ggtgagaaaa agctatctcg ataatccact gcaaccgacg aaaggtcgtg gcgaagatac
    17221 ctatgtacag gtgagctggg aacaagcatt aaaactgatt catgagcaac acgatcgcat
    17281 tcgcaaagct aacggaccat cggctatttt tgccggatca tacggctggc gctcttctgg
    17341 cgtgttgcat aaagcgcaaa ccttactgca acgttatatg aacctggcgg gcggttattc
    17401 cgggcatagc ggcgattatt caaccggggc ggcacaggtg atcatgccgc atgtggtcgg
    17461 ttctgtagag gtgtatgaac agcagacctc ctggccgctg attctggaaa acagccaggt
    17521 tgtggtgctg tggggaatga acccgctgaa taccttaaaa attgcctgga gcagtaccga
    17581 tgagcaaggg ctggaatact tccatcagct gaaaaaatct ggcaaaccag tgattgccat
    17641 tgatcctatc cgctccgaaa ccatcgagtt ctttggtgat aatgccacct ggatcgcgcc
    17701 gaatatgggc accgacgtgg cactgatgtt agggattgcg cataccctga tgacacaagg
    17761 taaacacgat aaagtgttcc ttgaaaaata caccaccggg tatccgcagt ttgaagagta
    17821 tctgacaggt aagagcgata atactccgaa gagcgcggcc tgggcggcgg aaattactgg
    17881 cgtaccggaa gcgcagatag tcaaactcgc tgaactgatg gcggcgaatc gcacaatgct
    17941 gatggctggc tggggaattc agcgccagca atatggtgaa cagaaacact ggatgctggt
    18001 gacgctggcg gcgatgttag ggcagattgg tacaccgggc ggcggttttg gtttctctta
    18061 tcattactcc aacggtggca atccgacgcg tgttggcggc gtgctgccgg aaatgtctgc
    18121 ggcgattgcc ggacaggcca gcgaggccgc tgatgatggc ggaatgacgg cgattcctgt
    18181 tgcccgtatc gtcgatgcgc tggaaaatcc tggtggcaaa tatcagcata acggtaaaga
    18241 acaaacgtat ccgaatatca aaatgatctg gtgggcgggt ggcggtaact ttacccatca
    18301 ccaggatact aatcgtctga tcaaagcatg gcagaaaccg gagatgatcg tcgtttctga
    18361 atgctactgg accgcagctg ctaaacatgc agatatcgta ttaccgatca ccacatcgtt
    18421 tgagcgcaat gacttgacga tgaccggtga ttacagcaac cagcatattg tgccgatgaa
    18481 gcaggctgtt gctccgcaat ttgaagcgcg taacgatttt gacgtatttg ccgatcttgc
    18541 tgaattactg aaacctggcg gaaaagagat ctataccgaa ggtaaagatg aaatggcgtg
    18601 gctgaaattt ttctatgatg ccgctcagaa aggtgctcgt gcgcaacgcg tgactatgcc
    18661 gatgtttaat gccttctggc agcaaaataa actgatcgaa atgcgcagca gcgagaagaa
    18721 cgaacagtac gttcgttatg gtgatttccg cgccgatccg gtgaaaaatg cgctgggtac
    18781 gccaagcggc aaaattgaga tttactccaa aacgctggaa aaatttggct ataaggattg
    18841 cccggcacac ccaacctggc ttgcgcctga tgagtggaag ggtaccgccg acgagaagca
    18901 gttgcagctt ctgaccgcac atccggcaca ccgtttacac agtcagctta actatgcggg
    18961 actgcgtaaa aaatatgcgg ttgccgatcg tgaaccgatc actattcaca ccgaagatgc
    19021 tactcgattt ggtattgcga atggcgatct ggtgcgtgtc tggaacaaac gtggtcagat
    19081 actaacaggt gcggtggtga ctgacgggat caaaaaaggc gtggtatgcg tgcatgaagg
    19141 tgcatggcca gatctggaaa atggcttatg taaaaacggc agtgcgaacg tgttaacggc
    19201 ggatatcccc agctcgcagc tggcaaatgc ctgtgccggt aactctgcgc tggtgtatat
    19261 cgaaaaatat acgggtaacg caccgaagtt aacggcattt gataagcctg ctgttcaggc
    19321 gtaagaagtg aatagcgctc ggaaacgggc gctgaggtta tgcaaacgta aaactgcctg
    19381 atgcgctatg cttatcaggc ctacatagtc cctgcaatag attgaattag caagggtttg
    19441 taggccggat aaggcattca cgccgcatcc ggcagtaata ccagaccttt acggcttgcg
    19501 cgcaatcagc accgcgcgtt taggcgcagg ataaccttca actgtcttac tcggatcatg
    19561 tgggtcgagg aaatcggaca gagattcggt aaccatccat tcagtgcgtc gctgctcttc
    19621 agtagtggta acgcacacat ccacaatgcg gatatctaca aaaccacact tcttcagcca
    19681 gtttttcagc gccagcgcgg aagggatgaa atagacatta cgcatttgcg cgtaacgatc
    19741 gcctggtacc agcaccgtgt tctcatcacc atcaataacc agcgtttcca gcaccagttc
    19801 accctcattc accagttgat ctttcaactg ccagagatgc tccagcggcg aacggcgatg
    19861 gtaaagcacg cccatcgaaa agacggtatc aaaggctttc agtgccggaa gctgttcaat
    19921 acctaacggt atcaaatgtg cgcgctgatc gttacccagt agtttacgca ccgcttcaaa
    19981 ctggcacaga aacagttgcg tagggtcgat ccccaccgcg aggtgcgcgc ctgcgccaat
    20041 catgcgccac atgtgataac cgctgccgca gccgacatcc agaatggtgc gtccggttaa
    20101 atcagaaaga tggggcagaa cacgatccca tttccagtcg gaacgccatt cggtatcgat
    20161 gttgacgcca tacagtgaga acggcccttt gcgccatggc atcaggttcc gcataagcgt
    20221 ttcaatgcgt ttaatttgcc cggtgctcag tggctcttcg ctttcggcgg ttacgctatg
    20281 caataaatcc agacgatacg gtttaatttc aggcagaaat tccaccgcgt tggaccactg
    20341 cttaaacagc ccgtgctgct gctcgcgctg ccagttagca atctgcgcgg gcagcgtttc
    20401 gagccagtgt gaaagatgat ttttggcaat cagagaataa aagttaccaa agtcgatcat
    20461 gcagcgtcct ctgcttttaa tgccaccagt gaaccaaagt taaagcactg gaaccacagc
    20521 tcgctatgct caaaaccggc tttatgcagg cgtgctttat gggtttccac ggaatcggtc
    20581 agcatcacgt tttccagcat gctgcgcttc tggctgatct ccagttcgct gtaaccgttg
    20641 gcacgtttaa agtcgtggtg catgttgaac aacagttcac caactttggc atcttcgaaa
    20701 ctgaattttt ccgaaagcac cagcgcgccg cccgggttca gtccttgata aattttatcc
    20761 agtaatgcct ggcgctcgga aggttccagg aattgcaggg taaaattcag caccaccatc
    20821 gatgcgtttt caatggcgat atcgcgaata tcaccttcaa tgacgtctac tggcgtaggg
    20881 gctttatagg cgtcaatatg acgacggcag cgttcaatca tcgccgggga gttgtcgatg
    20941 gcaataattt tgcaattatc atgatgaatg ttgcgacgca ccgagagcgt cgccgcgccc
    21001 agagaacaac ccagatcgta aacctgcgta ccaggttgaa cgaagcgctc ggctaacata
    21061 ccaatcatgg aaataatatt ggaatagccg ggaacggaac gctggatcat atccgggaag
    21121 acttcagcta cccgttcatc aaaggtccag tcgcccagtc tggcgatagg ggcagaaaat
    21181 agcgtgtcgc ggtgagacat aacgtaaaaa tccgggaaaa agaaagtggc gtattgtgcg
    21241 ctaacgcagg gagaaaacca actcccaggg catataccaa agattcgcca gcaccatcag
    21301 caacagcgca caccaggtgg cgctcatgcc ggaacgtcgc cagcgaaaca gacggtgatg
    21361 aaaaccgtaa taatgcatca gacgaccagc aagcaaaacg atgccgcaaa tatgcaccat
    21421 ccaggtttct gcgccattca tttccataaa cagcatcaac acaatcgcga tgggaatata
    21481 ttccaccgcg ttaccatgaa tgcgaatagc gctttgcagt tcgctaaaac cgccgtcgcc
    21541 ataggcaacg cggtactgca aacgcaagcg aacgacatca aaagagaact tcattaataa
    21601 caacgcactt aaaacggcat acagcgcgct taccatacaa actcccttta aaatggccga
    21661 ttgcacctgt ctatgatagg tggcaaattc agaaaagaga agattgctgt ggaatagccg
    21721 gaactgctcc gatctctgga agcgttttac gtaagtcttc ccacagggta tgtaccagtt
    21781 ccggggcctg ggcaatatct ggcgtatgta aaaaaagata aggcgtagtg gtctgatgcc
    21841 actgcgctaa tttctgtaac cagacctgaa ataattcccg gttttgcgtc atatcatcac
    21901 taccgataaa acgaatcagc ggatttgtcg ccgtcagtac agcatgtacc ggaactttag
    21961 gtttttttcg ttgagcgtcg cgaatagctt cactgtgtgg acatgctgca tgaaccgggc
    22021 ggctgtctaa aatcacccga ttaacgccgc gctgatgtaa accgcgatta agcgtttgtt
    22081 cctcttcccc tttggcgaaa aactgtggat ggcggacttc cacaccataa ttaaattcgc
    22141 cagggagaga atcgagaaaa tgccaaagcg caggcagctc ccgtgggccg aatgtggcag
    22201 gcagttgcag ccagtattgc ccaatgcgcg gagccaacgg tgacatgcgg gtcaaaaatt
    22261 cagtcactaa atcatcgcaa tgccgtaatg ctgcctgatg cgaaatggtc gccggaaact
    22321 taaaacagaa gcggaagtca tctgtggtct gctcacgcca gcgcaggaca acctcgggtt
    22381 tcggcagggc gtaaagcgtg gtgttgccct ccacgcagtt aaagtggcgg gcatactctt
    22441 caaggctggt gatccccaac cgcacccatt taggatgcga ccattgcggt agaccgatgt
    22501 aaatcataac gcgtgaagga tctcttccac actgcgcaca cgggcgatgc gtgggtagat
    22561 atggttgatg ctgttattgt gctgctcggc gctggcggca ctacaggcgt cttcggcaat
    22621 caccagatta aagccaagtt cccaggcatt gcgggcggtg gattcaacac cgatattggt
    22681 cgagatccca cataacacta ttgtatcgat acctcggcgg cgcagttgca actccagatc
    22741 cgtaccgtaa aacgcacccc actgacgttt gatgatttcg atatcactgt cagttgcacc
    22801 taatgcagct ggatgttgcc accagttttc gggcaacact ttagcggggg agggggcatc
    22861 aaccggctgt tttaatgctt cggcgtaatc ggcagaccag ccaacgcgca ccagaaacac
    22921 gggctgaccg ctggcgcgaa atttcgccgc cagcttcccg gcgcgattaa ctacctcatc
    22981 ggcagtatgt ggaccaccgg caaaaggtaa gatgccttct tgtaaatcga tcaccaccag
    23041 cgcagtggtt ttagcattaa gttcaagcat aatgactcct gaaaaaacaa aatcgtgcct
    23101 cacaccttaa tgcgattagc cgatggcgga acgacaaatt ttgttaattt ttatgagttc
    23161 gggcaacacc tttatagcaa actggcgtag agtctgattt caggcccgat ataaagtggt
    23221 aacgaggcgt tccagcgcgg gccaggctct tacggtgcgg cagaatttcc agtataatag
    23281 ccgccttttt tcattcagtt gtgacataca gctaacgctg cgacttggtc acctgcgcag
    23341 cagggggcat ccgccggcag ctaagttaag ggatatctca tgcgtacaga atattgtgga
    23401 cagctccgtt tgtcccacgt ggggcagcag gtgactctgt gtggttgggt caaccgtcgt
    23461 cgtgatcttg gtagcctgat cttcatcgat atgcgcgacc gcgaaggtat cgtgcaggta
    23521 tttttcgatc cggatcgtgc ggacgcgtta aagctggcct ctgaactgcg taatgagttc
    23581 tgcattcagg tcacgggcac cgtacgtgcg cgtgacgaaa aaaatattaa ccgcgatatg
    23641 gcgaccggcg aaatcgaagt gctggcgtcc tcgctgacta tcatcaaccg cgcagatgtt
    23701 ctgccgcttg actctaacca cgtcaacacc gaagaagcgc gtctgaaata ccgctacctc
    23761 gacctgcgtc gcccggaaat ggctcagcgc ctgaaaaccc gcgctaaaat caccagcctg
    23821 gtgcgccgtt ttatggatga ccacggcttc ctcgacatcg aaactccgat gctgaccaaa
    23881 gccacgccgg aaggcgcgcg tgactacctg gtgccttctc gtgtgcacaa aggtaaattc
    23941 tacgcgctgc cacaatcccc gcagttgttc aaacagctgc tgatgatgtc cggctttgac
    24001 cgctactatc agatcgttaa atgcttccgt gacgaagacc tgcgcgctga tcgtcagcct
    24061 gaatttactc agatcgatgt ggaaacttct ttcatgaccg cgccgcaagt gcgtgaagtg
    24121 atggaagcgc tggtgcgcca tctgtggctg gaagtgaagg gcgtggatct gggcgatttc
    24181 ccggtaatga cctttgcgga agcagaacgc cgttatggtt ctgataaacc ggatctgcgt
    24241 aacccgatgg aactgaccga cgttgctgat ctgctgaaat ctgttgagtt tgcagtattt
    24301 gcaggtccgg cgaacgatcc gaaaggtcgc gttgccgctc tgcgcgttcc gggcggcgca
    24361 tcgctgaccc gtaagcagat cgacgaatac ggtaacttcg ttaaaatcta cggcgcgaaa
    24421 ggtctggctt acatcaaagt taacgaacgc gcgaaaggtc tggaaggtat caacagcccg
    24481 gtagcgaagt tccttaatgc agaaatcatc gaagccatcc tggatcgtac tgccgcgcaa
    24541 gatggcgata tgattttctt cggtgccgac aacaagaaaa ttgttgccga cgcgatgggc
    24601 gcactgcgcc tgaaagtggg taaagacctt ggtctgaccg acgaaagcaa atgggcaccg
    24661 ctgtgggtta tcgacttccc gatgtttgaa gacgacggtg aaggcggcct gacggcaatg
    24721 caccatccgt tcacctcacc gaaagacatg actgctgcag aactgaaagc tgcaccggaa
    24781 aatgcggtgg cgaacgctta cgatatggtc atcaatggtt acgaagtggg cggtggttca
    24841 gtacgtatcc ataatggtga tatgcagcag acggtgtttg gtattctggg tatcaacgaa
    24901 gaggaacagc gcgagaaatt cggcttcctg ctcgacgctc tgaaatacgg tactccgccg
    24961 cacgcaggtc tggcattcgg tcttgaccgt ctgaccatgc tgctgaccgg caccgacaat
    25021 atccgtgacg ttatcgcctt cccgaaaacc acggcggcag cgtgtctgat gactgaagca
    25081 ccgagctttg ctaacccgac tgcactggct gagctgagca ttcaggttgt gaagaaggct
    25141 gagaataact gatatgactc aaatacacga aatcattcgc gttgcatcaa ggcggcaact
    25201 gagtgaactc ccatgagcat agataactat gtgaatggga tgagcgaagg cagtcaacga
    25261 agaggcagcg tggaggataa agtgtataag cgtcccgttt cgatcttagt ggtcatctat
    25321 gcacaagata cgaaacgggt gctgatgttg cagcggcgtg acgatcccga tttctggcag
    25381 tcggtaaccg gcagcgtgga agagggtgaa accgcgccgc aagctgccat gcgcgaagta
    25441 aaggaagagg tcaccattga tgttgtcgct gaacaactga ccttaattga ctgtcagcgc
    25501 acggtagagt ttgaaatttt ttcacattta cgtcatcgct atgcgccggg cgtgacgcgt
    25561 aatacggaat catggttctg tcttgcgctt ccgcacgagc ggcagatcgt tttcactgaa
    25621 catctggctt acaagtggct tgatgcgcct gctgcggcgg cgctcactaa gtcctggagc
    25681 aaccggcagg cgattgaaca gtttgtaatt aacgctgcct gaacaggcag ggctatctgg
    25741 agatattttt atggcaggtc atagtaaatg ggccaacacc agacatcgta aagctgcgca
    25801 ggatgctaag cgcggtaaaa tcttcactaa aatcattcgt gagctggtaa ccgcggctaa
    25861 gctgggcggt ggcgatccgg acgctaaccc gcgtctgcgt gcggcggtgg ataaagcact
    25921 gtctaacaac atgacccgtg acacactgaa ccgcgcaatt gcacgtggtg tgggcggtga
    25981 tgatgatgca aacatggaaa ccatcatcta cgaaggttac ggtcctggcg gcacggcaat
    26041 catgattgaa tgtctgtctg acaaccgcaa ccgtaccgtt gctgaagtgc gtcatgcatt
    26101 cagcaaatgt ggcggtaacc tcggtacaga tggttccgta gcctatctgt tcagcaaaaa
    26161 aggcgtgatc tccttcgaga aaggcgatga agacaccatc atggaagcag ctctggaagc
    26221 aggtgctgaa gacgttgtga cctatgatga cggcgcgatt gatgtctaca ctgcatggga
    26281 acaaatgggt aaagtgcgcg acgctctgga agcggcaggt ctgaaagcag acagcgcgga
    26341 agtttccatg atcccgtcca ccaaagctga tatggatgca gaaaccgcac cgaaactgat
    26401 gcgtctgatc gatatgctgg aagattgcga cgacgtgcag gaagtttacc ataacggtga
    26461 aatctctgat gaggtcgcag cgactctctg atgaggcctg ctaaacagca aaacggagac
    26521 gcgtgatggc tattattctc ggcattgatc cgggttcgcg cgtgaccggc tatggcgtaa
    26581 ttcgccaggt aggcaggcaa ctgtcctacc tgggtagcgg atgcatacgc accaaagtgg
    26641 atgatttacc gtctcgtctg aagctcatct atgcgggcgt gacggaaatc atcacccagt
    26701 tccagcctga ttatttcgcc attgaacaag tctttatggc gaagaacgct gactcagccc
    26761 tgaaactggg ccaggcgcgc ggcgtggcga ttgtggcggc ggtgaatcag gaattgccgg
    26821 tatttgaata cgcggcacgt caggtaaagc aaacggtggt aggtattggt agcgccgaga
    26881 aaagccaggt gcagcatatg gtccgcacct tgctgaaact gcccgctaat ccacaagcgg
    26941 atgccgctga tgcgttagct atcgcgatta cccattgtca cgtcagccag aatgcaatgc
    27001 agatgagcga atcgcggctg aacctggcga gagggcgact gcgttaagtt ataccgcctc
    27061 ggtcagttca ggctgaggcg tttcaactcc ctccgcgtga atcaacacca aacccggatg
    27121 atgccacaaa ctggctggcg tgacggtttt acgctcccac ggaatagaac ctaagaacca
    27181 gaatttccag aaagtgagtt ttgcattcgg attgctattt aacagttgtc caaacgtctc
    27241 tatttcaccc accggaatac atagcgcctc tttataaata tcaaagacaa attttgaaca
    27301 gaactggcgg gaagattcgt atttaaaacc cgtgtggtaa agtttgcgta gccgggaggg
    27361 aacctgttca acaattcgtt gtttctgttg ttctgtcagc ccggcgtcta atcgctttat
    27421 agcatagcgt tgattagcag agcgtttaat aaaacgggat agcgtagtga tggttgagag
    27481 gggaacacgg ctttctgcaa ccagaaagtc ttcaccgtta tgaccgataa tgatcccgac
    27541 gtgattactc cagcagtttg atgcagctga tatttgacca aataaggcag cacctataca
    27601 tgtaaagacg atatcaccaa tttcatattc agcagggtaa ttaatattca cgttgtcatt
    27661 ccattgaaat agatatgaat atacgtaata gcataaaaaa tttattattt caggagtcgc
    27721 aatgtatatt tttattacgc atttcttcac tgaatatgta atatttaaat atttgcttcc
    27781 aatataacct gttgaataaa ttatactctg ccatttttca gatcatcggg acacctcgca
    27841 agttttcttc atccttcgct ggatatctat ccagcatttt tttatcatac agcattatct
    27901 ttgattcatt acgcaggagc gtcatgtgat aggcagactc agaggcatca tcattgaaaa
    27961 acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc atatgccgat
    28021 gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg atcgttttca cccactttgt
    28081 ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat aaacaagagc gcacgttgtt
    28141 caaagagttg atcaaaacca acggcgtcgg cccgaagttg gcgctggcga tcctctccgg
    28201 aatgtcagcg cagcagttcg ttaatgccgt tgagcgtgaa gaagtggggg cgctggtgaa
    28261 actgccgggt attggcaaaa aaaccgccga acgcttgatt gttgaaatga aagaccgatt
    28321 taaaggtttg catggcgatc tctttacgcc agccgccgac ctggtactca catcacctgc
    28381 cagcccggcg accgacgatg ctgaacaaga agcagttgcc gcgctggtgg cgctgggcta
    28441 taaaccacaa gaggcaagcc gcatggtgag caaaatcgct cgccctgacg ccagcagtga
    28501 aacattaatt cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat tgaagcagac
    28561 cgtctgattt ctgccggtac cactttgccg gaagatgtgg cagatcgcgc cattcgccct
    28621 aaattactgg aagagtatgt tggtcagccg caggttcgtt cacagatgga gattttcatc
    28681 aaagcagcga aactgcgcgg cgatgccctc gatcatctgt tgattttcgg tcctccgggg
    28741 ttgggtaaaa ctacgcttgc caacatcgtc gccaatgaaa tgggcgttaa tttacgcacg
    28801 acttctggtc cggtgctgga aaaggcgggc gatctggcag cgatgctcac taaccttgaa
    28861 ccgcatgacg tgttgtttat tgatgagatc caccgtcttt cgccagtggt ggaagaggtg
    28921 ttgtatccgg caatggaaga ctaccaactg gatatcatga ttggtgaagg tccggcggca
    28981 cgctccatta aaattgattt gccgccgttt accctgattg gtgcaaccac gcgcgcaggt
    29041 tcgctgacat caccgttgcg cgatcgtttt ggtattgtgc agcgtctgga gttttatcag
    29101 gtgccggatc tgcaatatat cgtcagtcgc agcgcacgct ttatggggct tgagatgagt
    29161 gatgacggcg cgctggaagt tgctcgtcga gctcgcggta cgccgcgcat tgccaaccgt
    29221 ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat ctcggcagat
    29281 atcgctgctc aggcgttgga tatgttgaat gtcgatgctg aaggtttcga ttatatggac
    29341 cgcaagttat tgctggcggt aatcgataag ttctttggtg ggccggtagg tctggataac
    29401 cttgcggcag ccattggcga agaacgtgaa accattgagg atgtgctgga accttatttg
    29461 attcagcaag gctttttgca gcgtacaccg cgtgggcgta tggcgacgac gcgggcgtgg
    29521 aatcactttg gcataacgcc gccagaaatg ccgtaagtcg gattggtcgc tcgcgccgca
    29581 tccgacactg gcagtgtgtc tgatgcgaca ctgttcgcgt cttatcaggc cagccagaga
    29641 tagcgaaccg taggtcggat aaggcgctcg cgccgcatcc gacaaatgtg ttcagcgata
    29701 gattagctgg cctgcttttt catcatactg agaataaata acagtgccgc acatagcacc
    29761 accgacggac cagccggcgt atcgtaaaat gcggaaaagg ttaaaccgcc agttactgcc
    29821 accatcccca ccaaaacagc gacaccagcc atctgttccg gcgtgcgggc aaagcgacgc
    29881 gcagtagctg caggaataat cagcaacgaa gtaataatca acgcgccgac aaatttcatc
    29941 gctacaccaa tcgtcaatgc cgtcaccagc atcaacaaca atttcacgcg ctgtaatttc
    30001 acaccatcaa caaacgccag atccgggcta atcgtcatcg acagcaaatt gcgccattgc
    30061 cagaacaaaa tagccaccac gatgaccacg ccaatcgcaa tagcgatgag atcttctggc
    30121 gtcactgcca gcaaatcacc gaacaggtaa gccatcaaat caacacgaat attagacatc
    30181 agactaacga ccaccaggcc cagcgacagg gcactgtgcg ccataatccc taataacgtg
    30241 tcgatcgcca gctgtggacg cttctccagc cataccagac cgcccgccag cagcagcgta
    30301 acggcaatca ccgcatagaa tggattcacg tccagcaaca aaccaaacgc gacaccaagt
    30361 aatgaggcat gagccagcgt atcaccgaaa taagacatac gacgccagac tacaaacgaa
    30421 cccagcggac ccgcggcaca ggcgagcatg atcccggcta accaaccggg aaataataat
    30481 tcaatcatga gcgatcattt ccccgacgca aaacaattcg tccctgtaaa tcgtgacgat
    30541 gattatgatg atggcgatag atacccagtt gttcagcacc acgaggacca aacatagaaa
    30601 taaactccgg atgtagggaa acaacttccg gtgtgccgga acaacaaatg tggtgattca
    30661 gacacagcac ttcatcggtt tttgccatta ccagatgcag atcgtgagag accattaaaa
    30721 cgccacaatc cagttcgcga cgcagttggt caataaggtc atataacgcc acctggccat
    30781 ttacatccac gccttgagtg ggttcatcca gcactaataa ttgcgggcga tttaacaatg
    30841 ctcgcgctaa tagtacacgc tgcgtttcgc caccagagag cttttgcatc ggtgcgttaa
    30901 tcaggtgccc ggcctggaca cgtttcagtg caggcaaaat atcttcttta tgtgtaccag
    30961 ggcgtaagcg taaaaaacgg tttacggtca gtggcaacgt ggtgtcgaga tacagcttct
    31021 gcggtacata gccgatgcgc agttttccgt tgcgcttgat aaccccttca tcgggtgtta
    31081 ccagcccgag cactacccgt accagtgtcg acttacctgc gccatttggc ccaagtaaag
    31141 tcaaaatttt tccaggttta agttccagcg acacatcaga gaggacgcgg cgttggccaa
    31201 aagaaaccga gacattttcc agggaaacca gacttgtcat gttaatttta gtcttgcagt
    31261 agtcatgaaa tgttataata tcacacttct catattcatt acgatgattg gtcgcattat
    31321 gttacataaa aaaacgcttc ttttcgcagc attatccgcc gctctctggg gaggtgcaac
    31381 acaggccgca gatgccgccg ttgtcgcttc gcttaaaccc gttgggttca tcgcttctgc
    31441 cattgctgat ggtgtaacag aaacggaggt tttactgcct gacggcgctt cagaacatga
    31501 ttattcactg cgtccatcgg atgtaaaacg cttacagaac gcggacttag tcgtttgggt
    31561 tggcccggag atggaagcgt ttatgcaaaa accggtaagc aaattaccag aagcgaagca
    31621 ggtaacgatt gcgcagcttg aagatgtgaa accgctactg atgaaaagta ttcacggcga
    31681 tgatgatgat cacgaccacg cggaaaaaag tgacgaagat caccatcacg gcgatttcaa
    31741 catgcatctt tggctttccc cagagatagc gcgggctaca gcggttgcaa tccatggaaa
    31801 attagtggaa cttatgccgc aaagtcgagc caaacttgac gccaacctga aggattttga
    31861 ggcacaatta gcctcaaccg aaacgcaggt tggtaacgag ctcgcgccgc tcaaggggaa
    31921 aggttatttc gtttttcacg atgcttacgg ctatttcgaa aaacagtttg gactgacacc
    31981 gcttggtcat tttaccgtta accctgagat tcaacctggc gcgcagcgtt tacatgaaat
    32041 aagaacacag ttggttgagc aaaaagcaac ctgcgttttt gctgagccac agttcaggcc
    32101 agcggtcgtt gagagcgtcg cacgagggac atccgttcgt atgggaacgt tggatcccct
    32161 ggggacgaat atcaaactgg gtaaaacaag ctattcagaa ttcctgagtc aattagccaa
    32221 ccagtatgcg agctgcctga aaggagatta atgaggaagt gattacgtgc aacagatagc
    32281 ccgctctgtc gccctggcgt ttaataattt accgcgacca caccgcgtta tgttggggtc
    32341 gctcaccgtt cttactctgg ccgtcgctgt ctggcggccc tatgtttatc accgcgatgc
    32401 cacgccaatt gtcaaaacca ttgagctgga acagaacgaa attcgttcgc tcttacctga
    32461 agccagtgag ccgattgatc aagctgcaca agaagatgaa gccattcccc aggacgaact
    32521 ggatgacaaa atcgccggtg aagcgggcgt gcatgaatat gttgtttcca ctggcgatac
    32581 gctaagcagc attctcaatc agtatggtat tgatatgggg gatatcaccc aactggctgc
    32641 tgccgacaaa gaattgcgta acctgaaaat cggtcaacaa ctctcctgga cattaaccgc
    32701 ggacggcgaa ctgcagcgcc tcacctggga agtgtctcgt cgtgaaaccc gaacctatga
    32761 ccgtactgcc gctaacggtt ttaaaatgac cagcgaaatg cagcaaggag agtgggttaa
    32821 caatctgctg aaaggtaccg tcggggggag ctttgttgcc agcgccagaa acgccggttt
    32881 aaccagcgcc gaagtgagcg cagtgattaa agccatgcag tggcaaatgg atttccgcaa
    32941 actgaaaaaa ggcgatgaat ttgcggtatt aatgtctcga gaaatgcttg atggtaaacg
    33001 tgagcaaagc cagctgctgg gcgtacgttt gcgttcagaa ggtaaagatt attacgctat
    33061 ccgcgctgaa gatggcaaat tctacgatcg taacggtact ggtctggcga aaggattctt
    33121 gcgattcccg acggcgaaac aattccgtat ctcgtctaac tttaacccgc gtcgtactaa
    33181 tcctgtgacc ggtcgcgttg caccacacag aggtgttgat ttcgccatgc cgcagggtac
    33241 gccagtgctt tcagtgggtg acggtgaagt ggtggttgcc aaacgcagtg gcgcagcagg
    33301 ttattatgtg gctattcgtc atggtcgcag ctacaccacg cgttatatgc acttgcgcaa
    33361 gattctggtg aaaccgggac agaaggtgaa acgtggcgac cgtatcgcgc tttccggtaa
    33421 taccggacgt tcaaccgggc cacatctgca ctatgaagta tggataaacc agcaggccgt
    33481 aaacccgctg acggcaaaac tgccgcgtac cgaagggctg accggctccg atcgtcgcga
    33541 attcctggcg caggccaaag agattgtgcc gcagctacgg tttgattaat taacatccat
    33601 tcgcagccgg tacgcagtca gtaccggctt tttttatttg gtgcggggca agttgcgccg
    33661 ctacactatc accagattga tttttgcctt atccgaaact ggaaaagcat ggaaacgaaa
    33721 aaaaataata gcgaatacat tcctgagttt gataaatcct ttcgccaccc gcgctactgg
    33781 ggagcatggc tgggcgtagc agcgatggcg ggtatcgctt taacgccgcc aaagttccgt
    33841 gatcccattc tggcacggct gggacgtttt gccggacgac tgggaaaaag ctcacgccgt
    33901 cgtgcgttaa tcaatctgtc gctctgcttt ccagaacgta gtgaagctga acgcgaagcg
    33961 attgttgatg agatgtttgc caccgcgccg caagcgatgg caatgatggc tgagttggca
    34021 atacgcggac cggagaaaat tcagccgcgc gttgactggc aagggctgga gatcatcgaa
    34081 gagatgaggc gtaataacga gaaagttatc tttctggtgc cgcacggttg ggccgtcgat
    34141 attcctgcca tgctgatggc ctcgcaaggg cagaaaatgg cagcgatgtt ccataatcag
    34201 ggcaacccgg tttttgatta tgtctggaac acggtgcgtc gtcgctttgg cggtcgtctg
    34261 catgcgagaa atgacggtat taaaccattc atccagtcgg tacgtcaggg gtactgggga
    34321 tattatttac ccgatcagga tcatggccca gagcacagcg aatttgtgga tttctttgcc
    34381 acctataaag cgacgttgcc cgcgattggt cgtttgatga aagtgtgccg tgcgcgcgtt
    34441 gtaccgctgt ttccgattta tgatggcaag acgcatcgtc tgacgattca ggtgcgccca
    34501 ccgatggatg atctgttaga ggcggatgat catacgattg cgcggcggat gaatgaagaa
    34561 gtcgagattt ttgttggtcc gcgaccagaa caatacacct ggatactaaa attgctgaaa
    34621 actcgcaaac cgggcgaaat ccagccgtat aagcgcaaag atctttatcc catcaaataa
    34681 aaaacagcct ctcaaattat tgagaggctg tacttgataa aaaatctcaa tgagtgttct
    34741 caccattttg agtttttaga ttagtacgct attgagttaa tcgtagcgtg ataatttcaa
    34801 aaggtttgaa ttcaagattg caatattgat tgcaataaaa actgtcatct aatttatttt
    34861 cttggagatc gacatattga acgtagtgat aattacatac ccacgaaagc atggctgaaa
    34921 tttctaatcc ataactctca tataatctta cgattatacc gtcgccgtct tcagctaatt
    34981 ttattgtttc gatgataacg ctgttagtat cgatagcaaa aaatgatagt gaagggggta
    35041 gttcacccgg gtgtggtttg acagggacga tctgtaaggg atgattcatt atataggcct
    35101 ggttaacgac accacccaca cggtaatctc catgatgagg aaataaggag aatgtgaatt
    35161 tttgtttacc aatatctgca aaattattaa tcaggaaatt atcataatta tctgccttaa
    35221 caacttcccc aggtttgtgc tgcgaacgca acaaagttaa ctctagcgtc tggtctttga
    35281 cacgatagcc atatttgttt tttgctatta aagcaatgcc agtttcttga tcagaaaggt
    35341 cgacccagtg atgtgctggt atttcatctt tggcaaaagc gaacgaatca tcgctatgcg
    35401 ttggtctgtc aattacaccg aactgaattt gacattgtgc aagagatgtt tgtactgata
    35461 ccgggaattt tactttgaga gaaacattag gttcgtgcca atcaataaaa gtttggacat
    35521 caacgcgtgg actgtcctgt gtcagggtaa atgcgaagcg aagataagag gaattgtagt
    35581 gataaatcag cgtcatcgta gttttaggcc cgctgatcac agttgtcatt ttttcagcga
    35641 gtaaaacata tgattcccca tcacgataat ttgaagcaaa atcccaggcc gcgaaaaaac
    35701 ctgcatcttc gtggtatgca cggatctcac tgtgcatatt ttcagtaatg tattctttac
    35761 catatctttt atcatatagc gaaattaaat caccgctttc tgaaaaaaga agtttaatgt
    35821 aattattttc aatacttcga gattctgcct tcagaccatt tacgatctta ttctttggat
    35881 caatcacagc ataaccataa gagtttactc gtgctttgag ccagttgttg tttatattga
    35941 tccattcgtt acgagcaaaa caggtggtat taaataaaat gtgcggatct ttataagatg
    36001 aagtgtcaat tttacttaat aaagtagact gcgcatcaga aataattttc tcggtttttg
    36061 cttctagttt aagatattcc ttttcagttt catgataaac tcttgaaata catgagccgg
    36121 gaaggatgtc atggaattgc aaggtcagca tcgctttcca tatttcatca aactcactgc
    36181 gaagaattga cgtcatatta ttagtaatag ttataaaaaa ttcagcatca tggagtttat
    36241 tttccataat tcgattatgg gctttagttg ctgactctga tgtaaagcac ccttgatgag
    36301 cctcgaagta aagttcaccc gaaataatgg gcaaggattc gcgaaaggcg tcggcgtgag
    36361 tgaagaactt atcaacacgg ctgaaatcaa catgaggtaa gccatcaata ttgcgtatgc
    36421 gtgttaatcg ttctatatgt tcctcaccgg ggccagcgcc gccatcaccg acaccataca
    36481 ccataagcgc atagggacag atttcttttt cattatagtt ttgttcaata tattctaaag
    36541 agcgagcgcg ggctcggcta tcatacgtat cttcaggaaa gttatgcatc agaatttctg
    36601 agccatccag gccttgccag cggaaaaggt aattattaaa tttattaatc ttgttttgtg
    36661 agagcttttg cgatgcaata tagttaatgc ctgatttttt gagaatttgt ggaagcgtgg
    36721 cggggaatcc aaagacatcc ggttgccaga cataatttat cttctttgaa aaattctgca
    36781 gtgtgaatcg tgtcccatgc atgatttgcc ttatcagcga ttcaccactt accagattta
    36841 aatcgcattc gacccaaaag caaccttgta actcaaagcg gcctgcggcg atttgttttt
    36901 ttaacttttc gaagaaatag ggataatctt tttttatcca gtgaaaaagt tggtactgac
    36961 tagcgccaaa aatgtaatca ggatattttt caatgtttgc cagggcggtg gcaaatgttc
    37021 ttattgcttt tctgcgtcct tctcttaaag gccacatcca cgctatatct aaatgggcat
    37081 gaccagtagc cataatcctg aactctgaag gattattgtt tttactaatt atggattgga
    37141 ttgcaatttg gcatttaatt atttcttcat tgcaaaaggt aattaacgta ttatcaatat
    37201 attcgagaat tcttatgatt tcgtcagtac gctcgcttct tttggtgata attttttctg
    37261 ggataatacc tttaggatgt atggattcaa acttctgacc atcattaatc cagtcgaaaa
    37321 gcacttcaac atcataataa agggatttca gtagttgatt acatgtcgcg atatgaacat
    37381 cagtaataat ccctccatta gtgacatagc caaataaatc gttgcacgca gcatcaatcc
    37441 aaaactcgac ctcgtcattt tcactaacga gagaaaggtc aaccacccat ttacctggtt
    37501 tatctggcgg tacgccataa gatgatgctt tattcgtgat gccgcagatg ggtgaaccat
    37561 tcttatccac aataagacct tcgccaccga tatcaacgag agcgacgagt ttgtgtctaa
    37621 atttatctgc tggacgttta ccataaactc gcatccaggc acagtcaaac agttgacccc
    37681 agtctgagcc aatggtgagt ttctggtaag cgccgctacg gcgataaaca aaaggctgcg
    37741 gttcttttgt tatccatgct tcaatcgaca tttcagagac aacagtataa acacgctgac
    37801 ctattttatt cgacagtttg ataatattat cagtaaggta catagaaatc cctttaatta
    37861 attataatac tcgaaaatat taactctcta tattggagaa agttactttg cgaagaatgg
    37921 tttgaccaaa tggtataagt tttctgacta ttttagtctt gtttaaataa aaacagcctt
    37981 caatgtattg tttgccctct ggtgaggttg aatatgaaaa agattccata tcagcataag
    38041 agaactggat agattcatca gctctactac agggtatgta tcctgagtca aaatatttcc
    38101 cgttcagcag agtttttttg attaaaatat cgctctcaag ctcaattgca tataaaacgg
    38161 gacctctgga aatataagtg tcaccacata agtcagtatt aaactgtata tcagctataa
    38221 atttaatgtc aatcacatca tgatgttgcc atgttctatc taaaataata agtgagtttt
    38281 gagtatcatt tttagtaaaa gtaacaccat taataatcat catttttgcc cagccggggt
    38341 aacggaaact gagagcaaaa cgaacagatg actcaggctc aatgagacat tttacgctta
    38401 aatcatgagg gtattcagtt aattgtgtaa ttttaattgc aacattatta aatgtgccat
    38461 aaaactcaca cgggccataa aataatgcag taaaaccatt gttattttct tgaaacatat
    38521 tttcgacaaa agccggtatg gttcttgctg tattcggtac gcagcagaca gcagcctcct
    38581 catgagtagg ggagtattta tatctggtat taaactgatt actgtccgga tgtttccttt
    38641 catttgcttc ataacaatta tcagtcttac agtacattat cgcgtgaagt atgggatgct
    38701 gcatgccaaa actagcatta tagaatagcc actcagcatc atcggccaga ccaatattag
    38761 tggtcagttt tattaacata acataagtgc taaatagttc atgaacagaa caatattcat
    38821 agcctgtata agtggcatta gctgtgcgtc ctgtaatcca ctcatcacca atgggagcgc
    38881 cagatggtgt aagataatat ttcaactggg ataaaaaaat atctaatgca gtttgatact
    38941 gcggccgttg gctcgctgcg atggctaatg ccctaatatg ctcatacgta tgtacgccat
    39001 gacctttaag aaggtaacca gggtctatga catttttata ttgcagatct tgctccgaca
    39061 cgcatcctgc ggaataatct tcatacagcc acaagcaata tgagagatat tcaatgttat
    39121 tggttatttc ataaaggcgg tagaaaacat ctgttattgt taatccgtgg ctatgtccag
    39181 caaaaccctg ttccacaacg aaaggatgtg acgtttcttt agggtagcca gccataatgc
    39241 attgtgcggc ttgggtaaga gaagttaata aactctcatt acgggtggac tcataacatc
    39301 caagcacaaa acgcaaggca gtagcttgtg cccagaactc tccattttct gctttacagt
    39361 taaattttaa tgatgaatca taaattccaa tatagccatc atcatcttgc gtatcgataa
    39421 gttccaaaca taaatcgctc gctttttcct gccattcttt attatctaac aagtaggcac
    39481 tgcggcaata tccatccttc caattcgatt gagtttcact atcccaccaa taatattgca
    39541 tcatgttatc atcgggatta ttctgttttt taataacgcc atcttcagca gatacagtgc
    39601 gtggagttaa ccgatctctt ccatatattt tatgctccgt aataagatgg ggaactaatt
    39661 tatccagttc tcctatatga ccgttcatat accattccat cttattcttt aaccaaccag
    39721 tgg
//
LOCUS       DAEVZA010000030        39323 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEVZA010000030 DAEVZA010000000
VERSION     DAEVZA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.030"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            11..772
                     /locus_tag="LAF92_001625"
     CDS             11..772
                     /locus_tag="LAF92_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HBI9790650.1"
                     /translation="MQYGDMVEQDFLSLGLSDISAKVDPYRLIKYHFPGPGSINVAFS
                     ASSSGTKISQRECTDILFAEMKELAKMFSFFGQNKTLIEDLIEHFRYGNGSNFHSQQL
                     NLSFHEKINKYGYNSPIRIIKECIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFND
                     FEDSFNGLGISVHDISAQKISLLSFQNYAIGWSATIHFVAQDHFGLDVTDIKNKTYSK
                     YRFFRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            769..1260
                     /locus_tag="LAF92_001626"
     CDS             769..1260
                     /locus_tag="LAF92_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HBI9790651.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKY
                     DNF"
     gene            complement(1302..2252)
                     /gene="corA"
                     /locus_tag="LAF92_001627"
     CDS             complement(1302..2252)
                     /gene="corA"
                     /locus_tag="LAF92_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBI9790652.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2622..3386
                     /locus_tag="LAF92_001628"
     CDS             2622..3386
                     /locus_tag="LAF92_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBI9790653.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3533..5695)
                     /gene="uvrD"
                     /locus_tag="LAF92_001629"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3533..5695)
                     /gene="uvrD"
                     /locus_tag="LAF92_001629"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBI9790654.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKTMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5779..6495)
                     /gene="yigB"
                     /locus_tag="LAF92_001630"
     CDS             complement(5779..6495)
                     /gene="yigB"
                     /locus_tag="LAF92_001630"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBI9790655.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6495..7391)
                     /gene="xerC"
                     /locus_tag="LAF92_001631"
     CDS             complement(6495..7391)
                     /gene="xerC"
                     /locus_tag="LAF92_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBI9790656.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(7388..8095)
                     /locus_tag="LAF92_001632"
     CDS             complement(7388..8095)
                     /locus_tag="LAF92_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBI9790657.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(8092..8916)
                     /gene="dapF"
                     /locus_tag="LAF92_001633"
     CDS             complement(8092..8916)
                     /gene="dapF"
                     /locus_tag="LAF92_001633"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBI9790658.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8953..9156)
                     /locus_tag="LAF92_001634"
     CDS             complement(8953..9156)
                     /locus_tag="LAF92_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI9790659.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9506..10060
                     /locus_tag="LAF92_001635"
     CDS             9506..10060
                     /locus_tag="LAF92_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HBI9790660.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LDMTHEQYLKGMRLEKRNKTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            10432..11874
                     /locus_tag="LAF92_001636"
     CDS             10432..11874
                     /locus_tag="LAF92_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBI9790661.1"
                     /translation="MHFRNVVCLLSCSLFLSPAWGCKLSSVDPNEYQQQGKGVVYLRP
                     NQNTNLSLPNVNYKRLRRLANLLIDPATLEDWEEIPPLTDLSTDYDVRGANAWYPHYS
                     WHSDGRYIIYAGKIVQNPAGTPPVDVTSFKAWGDFAADKNSLYFRGKRTDDNGGDKPL
                     DSKTLHQRNLPPPWEPDVLGLILRDANFLYINGHRIAETNSFRVLAQKSWDKHGKFSM
                     KFSCIRKPFGPWDTLARTQTKIMLNGEQLDADPDTFTVVRWIPEALISWRDKNGFHRK
                     VLDQYALDSNEEYSCGSFNVKEHRVTWRSSFPGKGAECQQEEIPGLDPEQFHPISDAV
                     AQYQDKLYVIETTPFDELKLNIVTLDDPKLIINKRFNAGKRHGYLLTRKGDEFGDSGL
                     QVFESAGPLILFDNHIPSEREAHQGSSNPYIQKWLARDDKYVYRFDGMQLWRYHTADP
                     RAVRVVNDQLDGKINGDGEFIPTPRDEAKK"
     gene            11871..13181
                     /locus_tag="LAF92_001637"
     CDS             11871..13181
                     /locus_tag="LAF92_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HBI9790662.1"
                     /translation="MKVIKKSISLALILLGYTVAIPAVQAEGYEYYSNQGALVYGSNF
                     EGQKKIIAKFTGINSRDLYPVENISGEQERYIIEDEVTTIKEEYHWITDGHVILWRGK
                     IVSNPPGTPTVDVASFKALGRFAVDKYSLYFDGQRTESNSGASKVDLATLKAIEDNSS
                     TLVDRHNLYLLGRRQASSDDFSVLQSKLWGDIERFRPRNEGATSRDLLIRHRQDVFLN
                     GQRIADVDADTFHIIRWIPNSLLVYRDKHGEHRYAYGNPAGKMATTDDANSFEIGEKT
                     VRWRRQLARDDVWHTQLSWDVIWGAWQDIPNVDPEQFHLITDRIAQYQDRLYIIKLSP
                     VGEDQLNVITLDTPDLVVDHVFNGGKKHIYIIKDRTWVQDVHVIATHGPLTMAEGFAW
                     DDRYVYTWWGQRPSRTESPCPEQTVEQDDGIVIKTEASECHRSP"
     gene            13212..14720
                     /locus_tag="LAF92_001638"
     CDS             13212..14720
                     /locus_tag="LAF92_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBI9790663.1"
                     /translation="MINTSRQDGFMHYRNVVCLLACSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDEWDKEPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            14717..16030
                     /locus_tag="LAF92_001639"
     CDS             14717..16030
                     /locus_tag="LAF92_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBI9790664.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIKNEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTSDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSIFGEDQLEIIPLDTP
                     DLVIDRSFNGGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            16078..16398
                     /gene="cyaY"
                     /locus_tag="LAF92_001640"
     CDS             16078..16398
                     /gene="cyaY"
                     /locus_tag="LAF92_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBI9790665.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGKT
                     VCFR"
     gene            complement(16438..18984)
                     /gene="cyaA"
                     /locus_tag="LAF92_001641"
     CDS             complement(16438..18984)
                     /gene="cyaA"
                     /locus_tag="LAF92_001641"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBI9790666.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            19371..20312
                     /gene="hemC"
                     /locus_tag="LAF92_001642"
     CDS             19371..20312
                     /gene="hemC"
                     /locus_tag="LAF92_001642"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBI9790667.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            20309..21049
                     /gene="hemD"
                     /locus_tag="LAF92_001643"
     CDS             20309..21049
                     /gene="hemD"
                     /locus_tag="LAF92_001643"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBI9790668.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            21071..22270
                     /gene="hemX"
                     /locus_tag="LAF92_001644"
     CDS             21071..22270
                     /gene="hemX"
                     /locus_tag="LAF92_001644"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBI9790669.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            22273..23469
                     /gene="hemY"
                     /locus_tag="LAF92_001645"
     CDS             22273..23469
                     /gene="hemY"
                     /locus_tag="LAF92_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBI9790670.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            24148..24309
                     /locus_tag="LAF92_001646"
     CDS             24148..24309
                     /locus_tag="LAF92_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790671.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(24393..25628)
                     /locus_tag="LAF92_001647"
     CDS             complement(24393..25628)
                     /locus_tag="LAF92_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBI9790672.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(25775..25851)
                     /locus_tag="LAF92_001648"
     tRNA            complement(25775..25851)
                     /locus_tag="LAF92_001648"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25815..25817),aa:Pro,seq:tgg)
     gene            complement(25894..25980)
                     /locus_tag="LAF92_001649"
     tRNA            complement(25894..25980)
                     /locus_tag="LAF92_001649"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25944..25946),aa:Leu,seq:cag)
     gene            complement(26001..26076)
                     /locus_tag="LAF92_001650"
     tRNA            complement(26001..26076)
                     /locus_tag="LAF92_001650"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26041..26043),aa:His,seq:gtg)
     gene            complement(26135..26211)
                     /locus_tag="LAF92_001651"
     tRNA            complement(26135..26211)
                     /locus_tag="LAF92_001651"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26175..26177),aa:Arg,seq:ccg)
     gene            complement(26314..27699)
                     /locus_tag="LAF92_001652"
     CDS             complement(26314..27699)
                     /locus_tag="LAF92_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI9790673.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(27890..28630)
                     /gene="rffM"
                     /locus_tag="LAF92_001653"
     CDS             complement(27890..28630)
                     /gene="rffM"
                     /locus_tag="LAF92_001653"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBI9790674.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(28633..29985)
                     /gene="wzyE"
                     /locus_tag="LAF92_001654"
     CDS             complement(28633..29985)
                     /gene="wzyE"
                     /locus_tag="LAF92_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBI9790675.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(29982..31061)
                     /gene="rffT"
                     /locus_tag="LAF92_001655"
                     /gene_synonym="wecF"
     CDS             complement(29982..31061)
                     /gene="rffT"
                     /locus_tag="LAF92_001655"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBI9790676.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(31058..32308)
                     /gene="wzxE"
                     /locus_tag="LAF92_001656"
     CDS             complement(31058..32308)
                     /gene="wzxE"
                     /locus_tag="LAF92_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBI9790677.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(32310..33440)
                     /gene="rffA"
                     /locus_tag="LAF92_001657"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(32310..33440)
                     /gene="rffA"
                     /locus_tag="LAF92_001657"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBI9790678.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(33445..34119)
                     /gene="rffC"
                     /locus_tag="LAF92_001658"
                     /gene_synonym="wecD"
     CDS             complement(33445..34119)
                     /gene="rffC"
                     /locus_tag="LAF92_001658"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBI9790679.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(34097..34978)
                     /gene="rfbA"
                     /locus_tag="LAF92_001659"
     CDS             complement(34097..34978)
                     /gene="rfbA"
                     /locus_tag="LAF92_001659"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBI9790680.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(34997..36064)
                     /gene="rffG"
                     /locus_tag="LAF92_001660"
     CDS             complement(34997..36064)
                     /gene="rffG"
                     /locus_tag="LAF92_001660"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBI9790681.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(36064..37326)
                     /gene="wecC"
                     /locus_tag="LAF92_001661"
     CDS             complement(36064..37326)
                     /gene="wecC"
                     /locus_tag="LAF92_001661"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBI9790682.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(37323..38453)
                     /gene="wecB"
                     /locus_tag="LAF92_001662"
     CDS             complement(37323..38453)
                     /gene="wecB"
                     /locus_tag="LAF92_001662"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBI9790683.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLIDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(38509..>39323)
                     /gene="wzzE"
                     /locus_tag="LAF92_001663"
     CDS             complement(38509..>39323)
                     /gene="wzzE"
                     /locus_tag="LAF92_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBI9790684.1"
                     /translation="LRNLDVRSNMASADQPSVMDEAYKEFVMQLASWDTRREFWLQTD
                     YYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDSVKLIAETAPDANNLLRQYVA
                     FASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKAIYDRRMNSIEQALKIAEQHN
                     ISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGPAFDLDYDQNRAMLNTLNVGP
                     TLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGGLIGAGVALTRRCSK"
BASE COUNT         9963 a        10428 c         9834 g         9098 t
ORIGIN      
        1 tgctgatgat atgcaatatg gtgatatggt agaacaagac tttctctcac tcggcctaag
       61 cgatatctca gcaaaagttg atccttacag acttattaaa taccatttcc ctggcccagg
      121 ttctattaat gttgctttta gtgcctcaag ttccggaaca aaaatatcac aacgtgaatg
      181 cactgatatt ttatttgcag aaatgaaaga actagcaaaa atgttttcct tttttggaca
      241 aaataaaaca cttatcgaag atctgattga acattttaga tatggaaatg gcagcaattt
      301 tcatagccaa caattaaatt tatcttttca tgaaaaaata aataaatatg gttataatag
      361 tccaatacga ataatcaaag aatgtatcga aaatggcatt aattcaactc cctcgacagg
      421 ataccaacct cttatcctgc aaagcattaa aaccaaatta ctttcatcaa gactaaataa
      481 atttaatgac ttcgaggaca gttttaatgg cttaggaatc agtgttcatg atatttcagc
      541 tcaaaaaata tctcttttaa gttttcaaaa ctacgctata ggatggagtg caacaattca
      601 ttttgtagca caggaccact tcggtcttga tgtaactgat atcaaaaaca agacttacag
      661 caaatataga ttttttcgca tatggttctt tttacaacgg cataaagact ttgctttcaa
      721 accttttttc accaatttta acactatcga aagaatcgaa aattatctat gaaaagcaaa
      781 ctccagaaaa ttatattatg tttatttctt ctatgctgca tctataattt atggacattg
      841 cgtcctgtac agattttgta tacatactct gatgctggca attcagtctt tctggtcgtt
      901 gatcatctgc catggactga cagcgataaa ataaattggt atctgaagca tcagaatgaa
      961 attaaaaatc aacatcccct accagaaggt agttggcata cctggtatgt cattgatatc
     1021 ggtaatggct tcactgacta taaaaaatat atcgaagggc cgtatgaaga tctctactgt
     1081 ttcccgacaa ttaagagcaa tgataattgc atcgtgaaaa attacttaat ggttattaat
     1141 gaataccctt atcgtaacac tcatattggt attaacgatt ttactgagta tcaattaaca
     1201 caagaaaata aaatagagcg agttttcaat ccgcatgact ttaaatacga caatttttag
     1261 aaataagccg gataaaaaaa tagctttatc cggcactcat attacagcca gttcttgcgc
     1321 ttaaagtaca gatacggtgc caggcccgcg aggatcataa agataatcgc gcctgggtag
     1381 ccgaaactcc acttcaactc tggcataaat tcaaagttca tcccgtagct ggaagccacc
     1441 agcgtcggtg gcaggaacac cacggatacc accgagaaga ttttgatgat gcggttctgc
     1501 tcgatgttga taaagcccat cgccgcctgc atcaggaagt tcaccttctg gaacagggat
     1561 tcgttatgcg gcagcaggga ttcgatatct cgcaggattt cacgcgcctg ctccagttgc
     1621 ccacccggta aacgcgcctt gcgtaccagg aagttgagcg cgcgctgggt atccatcaga
     1681 cacaggcgaa ctttccagcc gatatcttcc agttccgcca gcgtggagag cgcctcgtcg
     1741 tactcatcgc cctgatgccc ttccataatc acccggctca actgctccag gtcgctatag
     1801 atattttcaa tttcatctgc caactgttcg attttggttt cgaacagatc cagcagcaac
     1861 tcgtaggcgt taccgtctac catcgactgg ctacgggcac gcatacgata cagacgaaaa
     1921 gcgggcagtt cacgctcacg cagagtaaac agacgaccat cacggatggt aaatgccaca
     1981 gtggagttac cggcgtgatc ttccgcatct tcaaagaaga agaaggagtg aatatgcagg
     2041 ccgtcgtcgt cttcaaagaa acgtgccgat gcttcgatgt cttccagttc cgggcgggtt
     2101 gccaggctct ggccaagttc agattgtacg cgcaatcgct cgtcgtcgtc cggttcgaca
     2161 agatcaatcc atactgcatt tacaaggggt tgtgactctt cgacttccag ccgggtcagt
     2221 cggttatttt ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc ttaaaatatc
     2281 ggacagttcg gtgggcacac agaaacaaat tgggtttcag accattaaac agcctgactc
     2341 agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag gctatcagca aaaagggata
     2401 gccttaggag ttgatcctgg atgacaggat agtgagccag tatctactgg gtgtgtccaa
     2461 ggcgaatgtc ctcttagagt gatcgtgcgc gcatgttacg ccagcaaaat tttgccgtca
     2521 acacgcaaca gaacaccaca gaacaatagt tgaacttcgc gctttttggt actcgtcaag
     2581 gtcgctaaca ggataaaaaa atccaacatc caggacctat aatggcgcat cggctactca
     2641 ttggtaaagg aatgatcacc ttgaatctca aacggatttt tctcaccctc accttacttc
     2701 ccttgtttgc tgttgccgct gatgattgcg cactctcaga tccgacgctg accgtacagg
     2761 cgtataccgt taatcctcaa acagagcggg tgaaaatgta ctggcaaaaa gctaatggcg
     2821 aagcgtgggg aacgttacat gctctgctgg cggatatgaa tagtcagggt caggtgcaga
     2881 tggcgatgaa cggcggcatc tatgatgaaa gctatgcgcc gctcggtttg tacatcgaaa
     2941 acggtcagca gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc
     3001 ctggcggcgt gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgccttca
     3061 aaaccagtaa agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacggtg
     3121 taattaatcc gcgtattcat cccaacgtcg cctcacgcaa aattcgtaac ggtgttggga
     3181 ttaataaaca tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt
     3241 ttgcctgtta tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga
     3301 tttcacatat gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca
     3361 tgatttccgt cgagcggaaa ggttagaatg atagttttgt gtcagatact aaatatgctg
     3421 ctgatgccgg atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa
     3481 tctgaatgat tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg
     3541 actccagccg ggcataagcc gccaccagcc atttaatacc ctggccctga aatgccacct
     3601 gcaaacggct atgctcaccg ctgccttcca tattgacaat ggtgccttca ccaaacttag
     3661 cgtggcgtac gcgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca
     3721 tccgctgatg gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact
     3781 cttccggcag ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac
     3841 ggcgagtttc cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg
     3901 ccagacggcg ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca
     3961 taccctcttc cataccgaca ataaacacct gcgggaactc caggcctttc gccgagtgca
     4021 gcgtcatcaa ctgcactgca tcctgccagg tatccgcctg cccctcgccc gcttccagcg
     4081 ccgcatggga gaggaatgcc tgcagcggca ttaaatcttc atcttcttcg ttgtagctga
     4141 actggcgcgt tgccgtcacc agttcctcta agttttcgat acgcgtctgg cctttttcgc
     4201 ccttctcctg ctcgtacatg gtgcgcaggc cggagtcttt aattacccgg tcagtctgta
     4261 catgcagcgg catatcggca gtttcttgcg ctaaggcatc gattaattcc ataaatcgct
     4321 gcaaggcgct ggcagctcgc ccggcgaggg ctttttcctg caacagttca cgacatgcct
     4381 gccagagtgt taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac
     4441 caataccccg cgttggcgta ttcaccacgc gctcaaaggc cgcgtcgtcg ttacggttgg
     4501 caatcagacg cagatacgag agcgcgtctt tgatttcctg gcgttcgaag aagcgcatcc
     4561 cgccgtaaat acggtacggc atactggcct gcaacagtgc ttcttccagt acacgcgact
     4621 gggcgttact gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg
     4681 ttttgatgcg gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg
     4741 aaataggctc accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt
     4801 tttcaatcag ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca
     4861 gacgaatagt ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg
     4921 ccccgcgcca gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt
     4981 cgcccgccag caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca
     5041 ccaggatatt ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca
     5101 gttcgtgagc gcgcagcagc agctcggcga agtccaccag gcccgcgcgg tcacacgctt
     5161 cctgatacgc ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa
     5221 tatgatgcgg acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg
     5281 gcggccactg cttctcgtcg aggttcatgg ttttgatcag acgcttaagc aggcgcagct
     5341 ggtcctcgct atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac
     5401 gcagcaggcg gtgcgccagc ccgtggaagg tgccgaccca catgccgccc tggctggtgc
     5461 ccatcagttg cccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcaccg
     5521 ccataatcga gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca
     5581 gtacgcgcgt cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg
     5641 ccaccgcttc gcgctgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca
     5701 ccgccgcgta aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta
     5761 tatatacaga tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag
     5821 atgcggcagt aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca
     5881 acaagcctgc attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac
     5941 atgtaagatc tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct
     6001 gaacggtttt gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa
     6061 accaaacagt tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc
     6121 cagctgtttt aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc
     6181 aaagttaatc attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc
     6241 gagcatcgct tgttcaatcg aacgaaaacg ccaacgcgtc acgtcgtgat aaatctcggg
     6301 ttccgcttcc cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag
     6361 cgccggatga taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg
     6421 acggttatcg taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg
     6481 ccggtaaaaa cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag
     6541 gcaaggtgtt gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc
     6601 agcagctcct gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag
     6661 tgacgtaatt tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca
     6721 aagcgtttct gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg
     6781 tcgtcttcgc taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg
     6841 ttgcgaccaa tcggcaggcg gcgctctttg ctgcctttcc ccattaccca cacttcacca
     6901 gactccaggt cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg
     6961 ccgtacatca cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata
     7021 tccagcagcc gattcatatc gtcgacgtcg atgtttttcg gcagatgacg cggcgctttc
     7081 ggtgccgaaa ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca
     7141 aaaaagctac gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg
     7201 cgactgcgta cagcaaaatt gcgcaccatc gtcacatcac attgctgcca gctttgcagg
     7261 ccgttttcgc tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagcagggtt
     7321 atcgggctaa gctggcgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt
     7381 aaatcggtca tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt
     7441 catgaagtaa ctgcgttcct tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca
     7501 gcacaacacc caaatcagca tcgcttccca gcatcgacat cgccaccgat cccactgctt
     7561 tcgcttcagg tagcaccacc agcagctctg gtccgttaag cggtccaaga tagtgctgtt
     7621 cctgccccaa acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag
     7681 tgtggttcga cggcgcacct aagcgccagc gatccggaaa caggcgcaga ctcgcaccag
     7741 ccaggccgag atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg
     7801 cggcggtgag actgcgctgc aggtagagta agcgataaaa caggccttcg ttggcgatag
     7861 cctgttccat caacaacgcc atgttctctt ccagaacatg aatatgatta cgtgcgcggg
     7921 ccatgtgcca ttcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt
     7981 ctactgcgcg cgcattacgg ataaaaaact caggattttt aatcagataa tcgacaaccg
     8041 cccggtcatc aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg
     8101 aataaatccg tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc
     8161 tttccaggcg atatcaagac gaccgccggg gagttccacg cgtacttctt cggccagcaa
     8221 accttgctgg atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc
     8281 tcctgcccca cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa
     8341 accgatattg gctcgctccg gaaaacgctc gtggctttcc agaacaggac caagcgtttc
     8401 taccgccgcg gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac
     8461 cacgccgcat aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt
     8521 agcgcgaaac ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag
     8581 atcatcatcg gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcgcg
     8641 cttattggtc agccctttca gacgcacaaa acgggcaaag cagcgcgcac cattgccgca
     8701 ctgcgccact tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc
     8761 atacggcggc tcaaccacca gcagttggtc aaaccctact cccaggtgcc gatcagccag
     8821 gcgacgaatc agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa
     8881 atcgttgcca aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac
     8941 agaaactgac ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt
     9001 atccggcacc gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt
     9061 atctgcaggc gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt
     9121 aattaataca gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc
     9181 gctttctgct ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta
     9241 tgaagtctga aaagtgagga aatgctgcga gtataacgct tacacgtggg gatttatgtt
     9301 ttgctggtag tttcgtgatc ggcacctcgc gccaggatgg ctttatgcac tatcgcaatg
     9361 tggtttgttt gttgtcttgt tcattgttct tatcgtcggc atggggcttt taatagagtg
     9421 gcgcaccatg caatttcgtt gtctgacctt agtcgccagt gacatacaac tttgccaacc
     9481 tggtgataag agggaataaa atctgatggg gctatttacc caactggaaa aatttgacca
     9541 aaagcttacg cgcggttatg cccgctgggg gcgctgggta tggcggacgt taattgtcgt
     9601 tccggtactg gtcgtgttgt ggaatatagg acaggcagta tggggaggac caagaggtgg
     9661 agtgattctg gaaatccaca gtgaaattga ccgtccgata ctgggattca gtgttaacgg
     9721 cgtggcgggc gcgaatgctt ttgcaaatgg tggcggttca atcacctgtt gtggtgatgt
     9781 cagtggggat acggcagagg ttatctggac attagatatg actcacgagc aatatcttaa
     9841 agggatgcgt ctggagaaac gtaacaagac tttgcctctc ccaaaaagag aatggggaga
     9901 aaattatctg catgtgcatt ttatgcctgg cgataaagtg ctgctggggt ggagtaaaga
     9961 ttctttttct ccgtatgaag atcttcacaa cggggggtac aagacccgcg ttcggcagga
    10021 tgtgaaagat aaactttatg gtacagggaa aatgaattga tagatttcta taaaaatcac
    10081 gttcggcaga aagcgtaata atgtggcgaa tgttagatac ccgtggtgga acctgggtac
    10141 aactcgtgaa agacaataaa gaaggcaacg gggggcggaa tcccaccttg cctcccttcc
    10201 aggggtcaat ccggaatatt tttatgctat tagccgtact atggggcagt tccgggattg
    10261 tatttatatc gtgaatgaaa atatcacatt tattccagca actatcagaa aatagcgatg
    10321 acctttatgg attctgaaaa gtgacgattt gctgcgagag ttacgcttac ttgtgaggat
    10381 ttatgttctg ctggtagttt cgtgatcggc acctcacgcc aggatggctt tatgcacttt
    10441 cgcaatgtgg tttgtttgtt gtcttgttcg ttgttcttat cgcctgcctg gggatgtaag
    10501 ctttcatctg tggatcccaa tgagtatcag caacagggaa aaggcgtggt gtacctgcgc
    10561 cctaatcaaa acaccaatct ttcgctaccg aacgtgaatt acaagcgcct gcgtcggttg
    10621 gcgaatttgt taattgaccc agcgacactg gaagattggg aggaaatacc gccgttaacc
    10681 gatctgtcta ctgattatga tgtgaggggc gcaaatgcct ggtatcctca ttactcctgg
    10741 catagcgatg gtcgctatat tatttacgct ggcaagatag tgcaaaatcc tgcgggtaca
    10801 ccgccggttg acgtcacgtc attcaaggct tggggcgatt ttgccgctga taaaaatagc
    10861 ctctatttca gaggcaaacg cacggatgac aatggtggtg acaagccttt agatagcaaa
    10921 acactgcatc agaggaattt accgccgcca tgggaacctg acgtcctcgg gctgatattg
    10981 cgtgatgcta acttcctgta tatcaacggg catcgtattg ctgagaccaa tagtttccgc
    11041 gtgctggcgc aaaagtcctg ggataagcac ggaaaatttt ccatgaagtt tagctgcatt
    11101 cgcaaaccat tcggcccctg ggatacgctg gctcgtacac agacgaagat tatgctcaac
    11161 ggcgaacaac tggatgccga cccggatacc ttcaccgttg tgcgctggat acctgaagca
    11221 ttaataagct ggcgagataa aaacggcttt caccgaaagg tactggatca atatgccctg
    11281 gacagcaatg aggaatatag ctgcggtagt ttcaatgtaa aggaacaccg cgtcacctgg
    11341 cgtagtagct tcccgggtaa gggagcagaa tgccaacaag aagagattcc cggcctcgat
    11401 cctgaacagt ttcacccaat tagcgatgct gtcgcgcagt atcaggacaa gctgtacgtc
    11461 atcgaaacaa cgccattcga cgagctcaag ctgaatattg ttacgcttga tgacccgaaa
    11521 ctgattatta ataaacgctt caacgccggg aagcgtcacg gttacttgct cacaagaaag
    11581 ggagacgagt ttggggattc tggtttacag gtgtttgaat ccgccgggcc actcatttta
    11641 tttgataacc acatccccag cgaacgggaa gcgcatcagg gtagcagcaa cccttatatc
    11701 caaaaatggt tagcccgcga cgacaaatat gtttatcgct tcgatggcat gcagctctgg
    11761 cgctatcaca ccgcagatcc ccgagcggtg cgcgtcgtga acgatcaact cgatggcaaa
    11821 attaacggtg atggcgagtt tatacccact ccgcgcgatg aggctaaaaa atgaaagtga
    11881 taaaaaaaag tatctcactg gcgctcatcc tgttggggta cacggtggca attccggccg
    11941 tccaggctga agggtacgaa tactactcta accagggtgc cctggtttac ggttcaaact
    12001 tcgagggaca aaagaaaatc attgccaagt ttacgggaat taacagccgc gatttgtacc
    12061 ctgtggaaaa tatctctggc gagcaggaac gatatattat cgaagatgaa gtcaccacaa
    12121 ttaaagagga gtaccactgg ataacggacg gtcacgtcat tctctggcga gggaaaatcg
    12181 tgagtaatcc tcccggtacg ccaacggtcg atgttgccag ctttaaggct ctgggccgct
    12241 tcgccgtcga taaatacagt ctctattttg acggtcagcg caccgaaagc aatagcggcg
    12301 cttcgaaggt ggacctggca accctaaaag ctatcgaaga taacagcagc acgctggttg
    12361 accgacataa cctttattta ttgggacgca ggcaggccag tagcgacgat ttcagcgtac
    12421 tacaaagtaa attgtggggg gatatcgagc ggttccgccc acgcaatgaa ggggctacct
    12481 cccgcgacct gcttattcgt catcgccagg atgtgtttct caatggtcag cgcattgcag
    12541 acgttgatgc tgacaccttt cacatcattc gatggatccc caattcatta ttggtctacc
    12601 gcgacaaaca tggtgagcac cgttacgcct atggcaaccc ggcaggaaaa atggcaacga
    12661 ccgatgatgc gaattcgttt gaaatcggtg aaaaaactgt ccgctggcgc aggcaactcg
    12721 cgcgggatga cgtctggcac acgcaactct cgtgggatgt catatggggg gcctggcagg
    12781 atatccccaa tgttgatccg gaacagttcc atttgatcac tgaccgcatc gcgcagtatc
    12841 aggatcgtct gtatatcatc aaactgtcgc cagttggcga agaccagctt aacgttatca
    12901 ccctggatac ccctgattta gtggttgatc acgtcttcaa tggtgggaaa aagcacattt
    12961 atattatcaa ggacagaacg tgggtgcagg atgttcatgt gattgctacc cacggtccac
    13021 tcaccatggc tgagggtttc gcctgggacg atcgctatgt atatacctgg tggggccagc
    13081 gtccttccag aactgagtcg ccatgtccgg agcaaaccgt tgagcaggat gatggcatag
    13141 tcattaagac cgaagcatca gagtgccacc gctcgccgta aaatgggtca ttatttttaa
    13201 cgggtagttt catgatcaac acctcgcgcc aggatggctt tatgcactat cgcaatgtgg
    13261 tttgtttgtt ggcttgttca ttgtttttat cgtcggcatg gggatgtcgg ctttccgcac
    13321 ctgaacataa tatttaccag aaacagggaa aaggcgtggt gtacctgcgc ccttataaag
    13381 aaacaaatct ttctctacct gacgtgaatt acaagagttt gcgtcggtta cccaatttat
    13441 taatagaccc gacaacacta gacgaatggg ataaagaacc gcctctgact gatttgacaa
    13501 ccgactacct ctatgagggc gcacaggcct ggtatcctca ttattcctgg catagcgatg
    13561 gtcgctatat cctctacgcg ggtgaagttg tgcaaaaccc gccagacaaa cctcccgtcg
    13621 atgtcgcctc gttcaaggcg tggggtgatt ttgccgcaga taaacacagt ctctatttcg
    13681 aaggcaagcg caccgataac aatggcgggg gaaatcgctt agatatcaaa acactgcatc
    13741 aggtggaatt ccggcctccc tgggatcctg acttactggg gctgatatta cgtgacgcta
    13801 atttcttgta tatcaatggg catcgcctcg cagatcccga tagtttccgt gtgctggcac
    13861 aaaagtcatg ggatcagcga ggaaagtttt ctaccgcgtt taatccctgt gtggctatgc
    13921 catttggccc ctgggatacc ctggctcgta cacggaccaa aatcctgatc aacggcgaac
    13981 agcttgatgc cgacccggac accttttccg tcgtgcgctg gatgcccggc tcactcttga
    14041 cctggcgtga taaaaacggg ctgcagcgta aagtcctcga caaggaaaat ctggagtggg
    14101 atgaagattt aacaaagcac tgtctggatt tttctctgct ggaaaagaaa gtgttctggc
    14161 gtaaaggacc tgcttgtaaa caggaagaat tacccggact cgatccggag cagtttcaac
    14221 ccatcagtga tgctgtcgcc cagtatcagg actcgcttta taccatcatc gaaacagagt
    14281 ctggtgaccg caagctggaa atcgtgaaac ttgatgatcc caatcttatt atcaacaaac
    14341 gtttcaacgc cgggaaacgc cacggctatt tacttacgcg tgccgaaggg tggccatacc
    14401 attccggttt acatgtgttt gaatctgacg gaccgctgat cttactggat aaccgttctc
    14461 cggatgaacg cgaagcccat cttaatgacc atcccttttt gcgcagatgg tatgcccgcg
    14521 ataaccgcta catttacagc tttgatggcg cgcagctctg gcgataccgc accgctgatc
    14581 cgaaacaagt tcgcttaatc tggaaggaac aacattcggg atatggctat ggcgtaaatt
    14641 acaaaacggg atatctggac ggaaaaatta ctgatgacgg cgaatttatt cctgccccgc
    14701 gcaatgaggc gacaaaatga agggtcaaat aatcacagtt gcactcatcc tgttgggaca
    14761 cacatttctg acgcctgctg tacaggccat agggttcgac tattacaatg atcacggtgt
    14821 tatgtcttac ggtaaaggtt attgggggga taagaaaatc attgcccagt ttccggagat
    14881 gagcagggcc gatttgcgcc ttgtgaaaaa tatctctggg aaaacgggtt atataccttc
    14941 cgatgaaata agcataaaaa atgaggaata ccactgggtg acagacggcc gcgttattct
    15001 ctggcgtggc aaaatcgtta gcaatccacc gggaaccccc actgtcgata ttgccagctt
    15061 tcaggctatg ggccgcttcg cggtcgataa atatagcctc tattttgacg gacagcgcac
    15121 cgaaagtaat agcggcgcgt cacgtgttga tctggcgaca ctaaaagcta tcgaaggtaa
    15181 ctctaccacg ctggtggata gtaaaaatct ctatttgtcc ggtcgacgtc aggggagcag
    15241 cagtaatgtt actgtattag aaaaaagatg gtggggtatt aatccacgtc ttatgagtgt
    15301 aaacagaaat ttgtattcca atgatctgct tatccgcagt gggcagaata tttatttaaa
    15361 tggcgttcac cttacggcga atgcagactc atttgagata attcgctgga tacctcattc
    15421 actgctggtt tttcgcgaca ataagggtat gcatcgttat ccctttggtc aattatcagg
    15481 caaagcgata cccgtagatg atgacgtctc ttttgaagta ggggaaagtc gcgttcgctg
    15541 gcgtaaacag ctcacgtccg accgtcagtg gagcaagtgg atagacctac caggtattga
    15601 acctgaacaa tttcatctga ttactggcaa tattgcgcaa tataaagatc ggctgtatgt
    15661 aacaaaatta tcgatatttg gtgaagacca gcttgagata atcccgctgg atacgccaga
    15721 cctggtcatt gatcgctcat ttaatggcgg caaacaacat gcttacttta tccgccaatt
    15781 acggtcaaag agcttgcaaa ttattccagt taacggtccg ctaactaaaa acgatcgctt
    15841 cgcttatgac gatcgcaatg tttatacatg gaccgataca gaggtaagga ttacgccctc
    15901 cccctgcccg gcgaaaactc gtgtcagaga ggagaacgta cgtgaagttc aaaacagaga
    15961 cattattatt ccgttgacgg atgactcatg ccggaacgca gcaacagacg ggcaaacttt
    16021 gaagccctga ccccataccg ctatactgcc gccatcactt aaagacagga tacaacaatg
    16081 aacgacagtg aatttcatcg cctggctgat caactctggc tgaccattga agaacgcctg
    16141 gacgactggg atggcgacag cgatatcgac tgcgaaatca acggcggcgt actgaccatt
    16201 acctttgaaa acggcagcaa aatcattatt aaccgccagg agccgctgca ccaggtgtgg
    16261 ctggcaacca aacagggcgg ctaccatttt gacctaaaag gcgatgaatg gatttgcgat
    16321 cgcagcggcg aaaccttctg ggatttgctg gaacaggcgg cgacacagca agcgggtaaa
    16381 acagtctgtt tccgctaaga ttgcatgccg gataagcctc gctttccggc acgttcatca
    16441 cgaaaaatac tgctgtaata gcggcgtatc gtgatcctga ttggcaggcg gcatgttacc
    16501 gatagatttt gtgcggaacg gaatcacctg ttcacgacca tcaaccttca caatctgata
    16561 gaactgcggc aggttgaagt tgatgaagct tgagccgtag gtaaaacggt catgcgatga
    16621 cgagtagaag cgactgacgt cacgtaccag ctcctctttg ctgccttcgc agtggtgata
    16681 cacctcaacc cggttgcttt cgtcgagaat gtagatatta aagccattct cgtcttgcgt
    16741 ttcttcgaaa aagaactgga tgatcccttc gctggcaaag ccgtccacca ccgccggtaa
    16801 tttgacgtga ttggtttcaa cctgcactga caggccgtgc agtttgttat gcgaaatcgc
    16861 gccataaaac tcgatggcgt tttccagttt ctgtaccgat acattcaggc gttcgaagaa
    16921 caacccccag gtttgaccag aaacgcgcag cgccttgaaa cgcccggttt cctggcgggt
    16981 gctggaaaga cgcaattcaa tacactcaga aaccagttgc tgcacgcgag tacgaattaa
    17041 gccgcgcaga tgctggctat aacagaagac ttccacgcta tctggcggtg cggcgtcctg
    17101 atgcattttg ccgagaatag ttttcagggc ttcgatcatc gattgctcgc cgttgaaatg
    17161 cagcgtacgc acttcgttcc acgagttgcg gtacagcagg tcaacgctac ctaccaggca
    17221 attttgattc tcgccaaagc tgaagacatc cagcttacgg aaatcgaaat gcaccacctg
    17281 attgcggaac gccgctgtcg ggtcatattc caggttaacg ataatcgcca gatggcggat
    17341 ctcacacggg ctgtagagcg ccttcggtgt cggtgcaggt aagcgcagcg ggaaatggtg
    17401 cgacacgtcg gcgaccatct cctgcaactt aggcaaatcg acaatgccgt tacctttaat
    17461 atacaaacgg gtgcgcgagg tcagcaggcc gttaaaccat gcccacgcca ccagtttatt
    17521 caggtaacgg ttatattcca gcggctgatg gctgatgatc gactcaatat ttggcgcgcg
    17581 gttatacaga taccaacctg aacggttagc acggcccggc ggcacataaa taaaggtcag
    17641 attcggttcc gagagatcgg gtgaaatctg cgggtttacc agcgtcactt tacctggtaa
    17701 tgcttcaaac gcggcataca gcttacgcgt cagcacgccg atatcctgcg gactggcgga
    17761 gacgctaagg ttattgcgac gcgcaaagcg gatcagatta cggtagctct gcatcatcgc
    17821 gtcgagcaac tcgttgtgcg cctcacgcac ctgatcaatc ttccagttag cgcggttatc
    17881 gagcattgcc agacgagctt cgtcccaacc ccactcgctc actaactggc tcaacactgc
    17941 gcgacgccag cctacgcagg cgcgttcacg gctgagcttt tcgcacactt ttaaatagaa
    18001 gcagcgacgt actaaatcca gacgggtaaa atcttcaatc gccgtcaggt attcagtaac
    18061 acgctccagc atcatgcagt atggatcgag accaaacgat acaatctcgc cgtcgtgcaa
    18121 acgctgtttg atatctttcg ccagcagacg tgggttcggg tattcccagg aataggcttc
    18181 cagcagcagt gttttcagta ccgctttgta tggggaatcg atactcttgt agagctgcca
    18241 aaggctggca ccaaagtact cttcagcaga aagcgagctt aagccaccga gatccagcca
    18301 ttcatttggc gtcagcacgc cctgcgcgta aagcgtcatc acatagtcgt cgtaatgctc
    18361 ttcttcgtcg cacggcacca tattccacag aatacgctta ccggcgagac gcacggcggt
    18421 acgataaaat tcgtcaagca gcagtatatg ctgggtggag ccacaatctt cgccccccag
    18481 gctgccgctt tcattatgac ggaagcggtt ttcatcaatc aggaagaagc tgacttccac
    18541 acccagcgag gcggcccagc tttccagcag actacattta cgttgtagta actggcgctc
    18601 ttcgctatcg agccaggatt gatgacagac ccagatatcc aggtcagagg aacaactttg
    18661 ccctacggac gaggtgctgc ccatggtgta tacaccagta attggaagct cacctttcgg
    18721 tgggtcctgt actgacattc cacgatacag ttcaagctcg ttcaggtagt ggcgttgagt
    18781 ttcatcaggc gtgtaaaggc aaatgccttt gggaacgtta ccatcaaggt aacccggcat
    18841 tagcggatgg tgatagtgca acaatgtcgg cagtagactg tagacctgtt ggaatgcagg
    18901 ccccatagca gcaagcgcgc gatccacacg caattgattt atggcatcca gtctctgttt
    18961 cagagtctca atatagaggt acaagacgta tcgcctgatt tgctacccgt catgactgtg
    19021 attccgccaa catcaacggt aacacgcggc attcgggata tttcgtatgt caaaggtaac
    19081 cgttaccact tttcgcgcct ggttttttta gtttcacgac gaaaaaatgg tctaaaacgt
    19141 gatcaattta acaccttgct gattgaccgt aaagaaagat gcgctacata caagtgtagc
    19201 accgtttatt ctctgtaaat tccttattac gacggcgtga aacgcctgtc aggatccact
    19261 gccagacctc attttacggt ttgcgcaggc gtctacgttt caccacaaca ctgacatcac
    19321 tctggcaagg atgttaggat ggaccacgga tgataatgac ggtaacaagc atgttagaca
    19381 atgttttaag aattgccaca cgccaaagcc cacttgcact ctggcaggca cactatgtca
    19441 aagacaagtt gatggcgagc catccgggcc tggtggttga actggtaccg atggtgacgc
    19501 gcggcgatgt gattcttgat acgccgctgg cgaaagtagg cggaaaaggc ttatttgtaa
    19561 aagagctgga agtcgcgctc ctggagaatc gcgccgatat cgccgtacat tcaatgaaag
    19621 atgtgccggt tgaattcccg caaggtctgg ggttggtcac tatttgtgag cgtgaagatc
    19681 ctcgcgatgc ctttgtgtcc aataattatg acagtctgga tgcgttaccg gcaggcagta
    19741 tcgtcgggac gtccagttta cgtcgccagt gccaactggc tgaacgccgc ccggatctga
    19801 ttatccgctc cctgcgcggc aacgtcggca ctcgcctgag caaactagat aacggcgaat
    19861 acgatgccat cattcttgcc gtagccggac taaaacgttt aggtctggag tcccgcattc
    19921 gcgccgcgtt accacccgag atttctcttc cggcggtagg acaaggtgca gtcggtattg
    19981 aatgccgcct tgatgatacg cgcactcgcg agctgcttgc cgcgctgaat caccacgaaa
    20041 ctgcactgcg cgttaccgca gaacgcgcca tgaatacccg tctcgaaggt ggatgtcagg
    20101 tgccaattgg tagctacgcc gagcttattg atggtgaaat ctggctgcgt gcgctggtcg
    20161 gcgcgccgga cggttcgcag attattcgcg gtgaacgccg cggtgcgccg caaaatgccg
    20221 aacaaatggg gatttcgctg gcagaagagc tactgaataa cggcgcgcgc gagatcctcg
    20281 ctgaagtcta taacggagac gccccggcat gagtatcctg gtcacccgcc cgtctcccgc
    20341 tggagaagag ttagtgagcc gtctgcgcac actggggcag gtggcctggc attttccgct
    20401 gattgagttt tctccgggtc gacaattacc gcaacttgct gatcaactgg cggcgctggg
    20461 ggagagcgat ctgttgtttg ccctctcgca acacgcggtt gcttttgccc aatcacagct
    20521 gcatcagcaa gatcgtaaat ggccccgact acctgattat ttcgccattg gacgcaccac
    20581 cgcactggcg ctacataccg taagcggaca gaagattctc tacccgcagg atcgggaaat
    20641 cagcgaagtc ttgctacaat tacctgaatt acaaaatatt gcgggcaaac gtgcgctgat
    20701 attacgtggc aatggcggtc gtgagctaat tggggatacc ctgacagcgc gcggtgctga
    20761 ggtcactttt tgtgaatgtt atcaacgatg cgcaatccat tacgatggtg cagaagaagc
    20821 gatgcgctgg caatcccgcg aggtgacgat ggtcgttgtt accagcggtg aaatgttgca
    20881 gcaactctgg tcgctgatcc cacaatggta tcgtgagcac tggttactac actgtcgact
    20941 attggtcgtc agtgagcgtt tggcgaaact cgcccgggaa ctgggctggc aagacattaa
    21001 ggtcgccgat aacgctgaca acgatgcgct tttacgggca ttacaataac tctcataaca
    21061 ggaagccata atgacggaac aagaaaaaac ctccgccgtg gttgaagaga ccagggaggc
    21121 cgtggacacc acgtcacaac ctgtcgcaac agaaaaaaag agtaagaaca atactgcatt
    21181 gattctcagc gcggtggcta tcgctattgc tctggcggcg ggtgtcggtt tgtatggctg
    21241 gggtaaacaa caggccgtca atcagaccgc caccagcgat gccctggcta accaactgac
    21301 ggcattgcaa aaagcccagg agagccaaaa agccgagctg gaaggcatta ttaagcaaca
    21361 agctgcacag cttaagcagg cgaatcgtca gcaagaaacg ctggcaaaac agttggatga
    21421 agtccaacaa aaggtcgcca ccatttccgg cagcgatgct aaaacctggc tgctagctca
    21481 ggccgatttt ctggtgaaac tcgccggacg gaagctgtgg agcgatcagg acgtcacgac
    21541 cgctgcagcg ttgctgaaaa gtgcagatgc cagcctggcg gatatgaatg acccgagtct
    21601 gattaccgtt cgtcgggcaa ttaccgatga tatcgccagc ctttctgcag tttcgcaggt
    21661 ggattatgac ggcatcatcc ttaagcttaa tcagctttca aatcaggtag ataacctgcg
    21721 tctggccgat aatgacagcg atggttcgcc gatggattcc gacggtgaag agctttccag
    21781 ttccatcagc gaatggcgta tcaatctgca aaaaagctgg cagaacttta tggacaactt
    21841 cattacgatt cgccgtcgtg atgacaccgc cgtaccgctg ttagcgccaa atcaggatat
    21901 ctatctgcgc gaaaatattc gctctcgcct gctggtcgca gcacaagctg taccgcgtca
    21961 ccaggaagag acttatcgcc aggcgctgga gaacgtctcc acctgggtac gagcttacta
    22021 cgatactgat gatgccacca ccaaagcgtt cctcgacgag gtggaccagt taagccagca
    22081 aaatatctcg atggatcttc cggaaaccct gcaaagccag gcgatgctgg aaaaactgat
    22141 gcagacccgc gtgcgtaacc tgctggcaca accggcagcg gggacaacgg aagctaaacc
    22201 tgcacctgca cctgcacctg cacctgcacc gcaaggtgat actccggcag ccgcgccgca
    22261 aggagaataa ccatgctaaa agtgttatta ctctttgttt tgctgattgc ggggatagtg
    22321 gttggcccga tgattgccgg tcatcagggt tacgtgctga tccagactga caactacaat
    22381 atcgaaacca gcgtcacggg cctggcgatt attttgattc tggcgatggt agtgctgttt
    22441 gccattgagt ggctactgcg gcggatcttc cgcacaggcg cacacacccg tgggtggttt
    22501 gtcggacgta agcgtcgccg tgcccgtaag cagaccgaac aggcgctgct gaaactggcg
    22561 gaaggcgatt atcagcaagt tgaaaagctg atggcgaaaa atgccgatca cgcggaacaa
    22621 ccggtggtga actatctact ggctgccgaa gccgcgcaac aacgtggtga tgaagcacgc
    22681 gccaaccaac atctggaacg cgcagcagag ctggccggca atgacaccat tccggtagaa
    22741 atcacccgtg tacgtctgca actggcccgt aatgaaaacc atgctgcacg ccacggcgtg
    22801 gataagctgc tggaagttac gccacgccat ccggaagtat tacgtctggc ggaacaggcg
    22861 tatatccgca caggtgcatg gagttctctg ctggatatca tcccatcaat ggcgaaagcc
    22921 catgttggtg atgaagaaca tcgtgcaatg ctggaacaac aggcatggat tggcctgatg
    22981 gatcaggcgc gtgccgataa cggtagtgaa ggtttgcgta actggtggaa aaaccaaagc
    23041 cggaaaacgc gtcatcaggt agcgttgcag gtggcaatgg cggaacatct tattgaatgt
    23101 gacgatcatg atactgccca gcaaattatc atcgatggcc tgaaacgcca gtacgacgat
    23161 cgcctactgc tgccgattcc tcgactgaaa accaacaatc cggaacagct ggaaaaagtg
    23221 ctgcgccagc agatcaaaaa cgtcggcgat cgcccgctgt tgtggagcac actgggccag
    23281 tcactgatga agcacggaga atggcaggaa gcatcgctcg ccttccgcgc ggcgctgaaa
    23341 caacgtccgg acgcctacga ttacgcatgg cttgccgacg cgctggacag actgcacaag
    23401 ccggaagaag ctgcagcgat gcgtcgcgac ggtttgatgt taacgttgca gaacaaccca
    23461 ccacagtagt tccttctcac ccggaggcaa gcacctccgg gacctttctg atgcataaaa
    23521 aaacgcctgc tcttattacg gagcaggcgt taaaacaggt ctgtatgaca acaagtgggt
    23581 gcttcactca acgttgtgtc catggtgtct gatgaggcat aagcgacatc tgtcagtgga
    23641 cgataagcac cgtaaacggc tctgcgtcat tccggagttt atgaggcact aaggcgaaca
    23701 taagagatgg aatgagcatc tacccgttta ttatgccaca gagaatcggg aaataacatc
    23761 ccttaacact tgttatgaga taattctgta atcctctttg cttcctgagt aataacttcc
    23821 tgagtgaata tttaacctga gcttgatcct acacatactt attatgaatg ataaaattca
    23881 ttcaattaat aacacatata ttaattgccg ttaaaactaa aaaaagcatc aataatcaac
    23941 gcgatataat aaacctgcct tacatatcaa ctgcgccaga ggtaggattg aaaacgctct
    24001 cctgattttc caattcattt tctggataaa taaataattt atttttgtca ctattattta
    24061 tgtaatcatc ctgtcaggga gagggatctc aattatcaat gcttaattac gtcatcattt
    24121 tgatgacatg aaaccggagc attttgcatg gaattttcgt tttcacccaa acttcttgtt
    24181 gttgctgtcg ccgccgctct tcctctcatg gccagcgcag cagatacccc gtcaactgcc
    24241 accgcacgca aaggctttgc cggatacgat cacccaaacc agtatccaca gaacgctgca
    24301 atttattgaa tttgcacgat catgtaggcc ggataaggcg tttacgccgc atccggcaat
    24361 caaccgcagg cggccacaaa atggccgctt ttttacttat tcaccaccag caaatgtgcc
    24421 tgcataatgt cactggccgg gcgaccgtgc gccagcaaat cagccattgc tttaagatat
    24481 ggcggtagat ggcggaaata acgctgatac ccggcacaca aataattcag ccccggtttg
    24541 ccgctggcat cgagcatgaa gcggtgtttc gggcagcctc cccagcacgc ttttaacacg
    24601 ttacaactgc gacactgcgc cggtaattgc ttaaatttat cttcaccaaa cacctgctgt
    24661 tgcggggaat cgatcatttc tgcaattgtt tgctgatgca tattccccag ccgatattgc
    24721 ggataaacat agtgatcgca ggcgtaaacg tcgccgttgt gctcaacaat caccgagcgc
    24781 ccacaggttg gctgatgatg gcaaaccgca cccggcgcac cgacaaaatt ggcaaacgcc
    24841 cattcgatat tcatcacgaa aatcttgccg acgtcgcgtt tgatccagtg gtcgaatatc
    24901 gccaccagaa attcaccaaa ttcatcgggg cgcaccgacc attccgtcag ttccccctga
    24961 atatcaccag gcgcatgtag tttcagtcca tcgctggcag ctgtttcatc agccaggcgc
    25021 tcgaccaccg gaataaactg gatgaattcg actcccgcat cgcacaaaaa atcataaacc
    25081 tgcaacggtt gctgcgcgct ggtgcggttg acgcagacca gcacgttata gtcgacatga
    25141 tgtttttgca gcagcgtcag ggcacgcatc accagcttat gcgttggtct gccacctttg
    25201 gtcacgcgat attgattgtg gatctcagcc ggaccgtcca gcgataaccc aacaagaaaa
    25261 tgattttctg ccagaaatgc acaccattta tcatcgagca gcacgccgtt agtctggaag
    25321 ctgttactta tcttcctgcc agcaccgtat ttcgcctgta actttacggc acggcggtaa
    25381 aactccagcc cgagtaacgt tggttcgccg ccctgccagg taaaagccac ttcgttttgc
    25441 ggttcgctgg cagcgatata gtgacggaca tacgcttcca gcgtgtcatc gtccatatgc
    25501 gtgactggct tttcgcggta aagggattgt ttttcgagat aaaaacagta gtcacagttc
    25561 agattacaat cggaaccact cggtttcgcc atcacatgaa aagcacgcgt tggaacctgt
    25621 tgcagcatgg tcactccttg cctgaagtca tccttcaata cgttaagggc gaaatcatac
    25681 cgtggggcaa tgagttatta cgggcgttta ttgcgaaggg agggtgacga aatgcacaga
    25741 aaacaaaaaa ccccgccgaa gcggggttca aaattggtcg gcgagagagg attcgaacct
    25801 ccgacccact ggtcccaaac cagttgcgct accaagctgc gctactcgcc gaaatactgc
    25861 tttttgaatt tttagttcaa ttctttaaag tcgtggtgcg agggggggga cttgaacccc
    25921 cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg ccaccttcgc
    25981 attgtcacaa cttctaataa tggggtggct aatgggattc gaacccacga caactggaat
    26041 cacaatccag ggctctacca actgagctat agccaccact acaaatcttg ttacgcggta
    26101 ttactaccac cgcagctcaa gcgccgggac taaatggcgc gcccgacagg attcgaacct
    26161 gagacctctg cctccggagg gcagcgctct atccagctga gctacgggcg cttagcgccg
    26221 ttgcgggggt ggataatacg gacttcccac ccctctgtct agtccctttt taaataaatt
    26281 gcgcgtttgg ttacggtttg tgcgttttgc tgcttattcc tccagtttat gcgctttccc
    26341 gtggcgatta aggccaaaaa ctttataaat cgccgtcacc gccagcataa agatgatgcc
    26401 aacaaacagc gacatacgcg tatcttcatt aaagtacatg ccgatcaaaa cgcaaatcag
    26461 gaatgccatt gttacgtagt tggcccacgg gaacaggatt gagcggaacg gatggctggc
    26521 aatcgccgct ttatgtgcac gccgaaaacg cagctggctt atcaatatca caaaccatgg
    26581 caccatcccc ggaagcacgc tggcactgta gacgtagaca aacacacgct gcggattggg
    26641 aatgatgtag ttcaggcatg agccaattag cagaatagca atagatactg ccacacccgc
    26701 aacgggtacg ccgtgacggg aaactttcgc catcgccgcc ggtaactgac ggtttttcgc
    26761 cagtgcgtag agcatacgtc cgcaactgta catgccgctg ttacagccag agagcgcagc
    26821 cgtcagcacc acaaagttga taatgcccgc cgctgcggta ataccgattt tggcaaaagt
    26881 cagtacgaac gggctgccgt tgctgcctat ttcattccac gggaagatgg tgacgataac
    26941 gaaaatcgcg cctacgtaga aaatcaggat ccgccacagc accttgccta cggcactgcg
    27001 cagcgtcacc tgcggattct tcgcttcacc ggcagtaatg ccaatcagct ccacgccctg
    27061 gtaggacgcc accacaatac acagcgcggt caggaaccct ttccagccac ccgcaaagaa
    27121 accgccatgc tctgtgagat tgctaaaacc aatcgactgc ccgccattgc caaagccaaa
    27181 gaaaatcacg cccaggccaa tgacaatcat cacgataatc gtggtgactt tgatcatcgc
    27241 gaaccagaac tcgatttcgc cgtacaaccg caccgccgcc agattcgcca acgccaccag
    27301 cgccactgcg atcaatgcgg gtatccactg cgccatctcc gggaaccaga actggacata
    27361 aacgccaatg gcggtgattt ctgagatccc caccgccatc cacataaacc aataagacca
    27421 ggcggtgaga taaccaaaga acgggctcat ataacgatgc gcataaacgg cgaacgaacc
    27481 ggtaaccggt tcgaggaaca acatttcgcc cattgaacgc atgatgaaaa agacgaatag
    27541 cccggcgatg atataggcca acaatacgga tggcccggcc catttcaagg tactggcggc
    27601 ccccataaac aggccgacgc caatggtgcc ccccagggcg atgagttcga tatgtcgagc
    27661 ttccagccca cgctgtagct ctggtttgtt atctgccata aatcctcgtg ttgtgtttgc
    27721 atgctttccg gtgttaccgg ttatcgttat gggtacatcg agtgttgcga atgttttcgt
    27781 aattcaggag aaatggcaaa taaagcatta aaaatttgaa tgctttgtgt aataaaaaag
    27841 cagacaggcg acggagtgac cactccgtcg ctttacaaag agaggaaaat cagaggttgc
    27901 cggtgtagtg ccagcgtaaa taacgcagca aacgaagctg acgcttaatg cggctcggct
    27961 gcgaaagcag gcggtagagc cactccagtc ccagcgtttg ccagattttc ggtgcgcgtt
    28021 ttacgtgacc ggtgaaaaca tcgtaagtcc cgccaacgcc catatacagc gcatctggat
    28081 gcaccagacg gcagtcgcgc atgaagatct cctgctttgg cgatcccatc gcaacggtga
    28141 cgatttgcgc tccgctggca tgaatgcgtt caaacagcgc ctgacgctgc tcgggtttaa
    28201 aataaccatc ctgactgcca acgatattca cattccattg gttgcgcagt ttagcttcag
    28261 tttgcgccag cacttcaggt ttaccgccca caagaaatac cggcgtccct tctttgcctg
    28321 cgcgcgccat cagctcttcc cagagatcgg caccggcaac gcgggaaacc tgcgcctgcg
    28381 ggtacttttt acgtactgaa cgtacaacgc tgatgccatc cgcatattta aattcggcag
    28441 cgttaattaa ctccctgacc tcggcgttat cttcaatggt cagcattttt tcggcattaa
    28501 tggcaaccag cgttccctgc ttaagctgcc cgtcagcaaa cagataatcg agggcgtgct
    28561 gcatatcacg ccaaccaatc aactgtaagc cacgcagcgt ataggtcggt gccgtggtgt
    28621 tgttattcat tgttatcctt caacctgcgt ccggagcgat gattttgtac gtttatgaat
    28681 gagtccggcg ctttcaaaaa gccagtacaa cagttttgcg atcatcagac atgcgccgaa
    28741 gaccacgata aaaaagacta cgcgtgagac aaacgaatcc agcccttcac gtgccagcac
    28801 gatcatattg aagatcgccc caaagcagaa actgtgcaat atcgcagcct tatagcgatt
    28861 agtctcgcgg ttgcccagct catacagcca gtcgaaccat ttgatgatca gtccaaccac
    28921 aatcgccccg agtgggatga acaacgcgcc gcccatcacc accagtgagc ctataagcgt
    28981 aggcgagatc gccagtccgg agtggttatt cagcacttcc caggtaaagt agttggctga
    29041 gttcagcacc atactcgggc gacccggcca cagccaggaa gggataaaga catagaaatc
    29101 gcggacaatt ggagccaggc cctggaagtc gatgttgtcg tagttctgca acagcaacgc
    29161 cagattctcc catggcgaga aggtgtcgcg agtgagatag agaaacgtat agaacgcttc
    29221 atcgccgctc acattcattc catagcgttt tagcgccagc cagaacatgc ccacaatccc
    29281 cagcacgccc gccgccgcca gcatccacaa cgaaatccag ccgcgaataa tgccaataaa
    29341 caggaagata gcgaatgcga tgatgatatt ggcgcgagtg ccgccgacaa tcatataagt
    29401 cagcaagcca aaggcgaccg tgctgacgag gaaaaacagc cacgctttgc tatcctggcg
    29461 cagaaagtag accaccagca tcgccgggat gaaaaagtaa aagaagcgtt ttaacgccac
    29521 gccggagact tcactggaga agatctgact gtaggagtta agccggaaca gcaaaaagcc
    29581 gttgtgcatg aagaagatgc cgacgcttac cagcgcgatg cccatcagga tcacccacgt
    29641 aagattggtc tccacgcggt tcatggtaaa cagcggacgg cgcggtacat cagcaacgcg
    29701 tttgcgtagg cgggttttgt aggtgacata gtaaaccgcg tagaagcagc ccgcagaaag
    29761 caacgcctgc aacaagattt ctggcggcgc gacaccaaca tcaaagcgaa ataccagcac
    29821 gctggtcagc gggaagccga agaaaaaggt gagcaaaaac agcaatgaaa agaagacatt
    29881 gaagttaaag cgcacacggc gaaactcaaa ccaggtcagc gtggcaataa acagcgtgca
    29941 gagcagccag acaacaaaca ggccactgaa ttgcagcaga ctcatgcgac ctccccggcg
    30001 gcaatcgcca acgcccgctg ccagccttgt agatagttag ggctaaagaa ggcaatggtg
    30061 tttttatcca ccgacgccaa ctggcgctgc gcttcacgca caatatcctc gttgagatcg
    30121 tcggtagtaa acagcaccgg caaatgctgt tccgtcatat cctgccagaa cggattttcc
    30181 cggttaagca cacaaggaat gcccgcctga atcagtaagc aaagcgtacc aatgccctgc
    30241 tggcgggcaa aaataaagta accaagatcg cactgacgaa gtagcgccag ataggcgtca
    30301 aattccagtt tttcgctcaa aatttgcaga ttttcttcgc tgaataactc cagccccgcc
    30361 tgacgaactt cctcaatgta cgcttcgtta ttaggcggat atcccatcgg caccaccact
    30421 tttaccgtat cgccaaattg ctgatgaacg gcgcacaagg cagcaatatg gtcattgctg
    30481 cggtcaccgg agttccccac cagaatggtc attttccctt cacgttgccg atcgttcgcc
    30541 atcgtattga gagaagggtc catccgcgtc gggaagtaca gcagttcgcc ccgcaccttt
    30601 ggatgcgttt tggcaaaaaa gctcaaatcg ccgcgggtgg caaatacaca gccgactcgc
    30661 ttttgcgcca ggcgacgtag tgggtaaaaa agcttatatc tcaagccact ggaaagctca
    30721 tacaggtctg ccccccagat atgccagaaa aactggctgg gcttaatgcc accactcagc
    30781 agagccagcc acagtgtggg attgaactga ccgtggaaga aaaaacgctg ctgacggtta
    30841 gcttttgctt tcgcgatgac cgcttccgcc agcgattttt tcccagggaa aaattgcaca
    30901 gaaagcgccg gacagctatc acttaagccg tcgtccttgc caacaaccat aaactcgcgc
    30961 gcgtgctcgc tcgtcgcggc cagcgcgtca ttgaaaaacc gcaaaacggt tcggttatgg
    31021 tgagggatat ccgatcccag tacgtgaatc agtacagtca tgcccgccta cgccagagta
    31081 aaaacacgcc acaacaaaga gaaaaataga cgatataagt tgccatatat gcctgcgccg
    31141 cgcccagtgc accatgcgca gggattagcc agtgggcaaa taccatcaat aaagtgaact
    31201 ggctgatttc cgccagaata taaaaccgca atgacgcttt ggcgatcacc agataaccaa
    31261 agacataagc gcccactttt aacacatcac ccactaactg ccaggcaaag agatcgcgca
    31321 tagcggtaaa tttattcgac aacagcagcc agatagcaaa atcacgcagc agccagacgg
    31381 taaaactcgc cgccgccact gccggtaaga cgaatttcag cgatttaacc acttcccggg
    31441 tgatatcgcg cttttccgtt agccgcgaca acgtgggcag caaatatacg ctgaacgatg
    31501 ccgtaataaa ttgcaggtag gcatcggaaa tactgctcac cccttgccag atccccactt
    31561 catcccagct atactgcgcc gcgagcagtt tacgcatcat gatgtaagca acaggcaagg
    31621 tcaccgacgt aatcaacgcc atgagcgtaa atttgctcaa ctgccctgcc agaccgttat
    31681 cccagctggg tttcagatag cttaacggga tgacaccacg tttgatcaac atgatggcgg
    31741 caggaattac caccagcgcg ggaatcagcg ccagacccag caacgcccct tcataaccgc
    31801 ccaaacggta actgacgtag tacgcgagaa cgccaatcaa gctgccgaca atcagcgata
    31861 acgcattacc tgcggcatcg cgaaagcctt tcatcagcgc cagtaacagg ttgccccagg
    31921 cgatccccat ttgcaccagc gccactaaac gcaccagccc ctgatagtcg gtattaccaa
    31981 acaatccctg gctgattggc gcagctgcca gcacaaaaac cagcgccatc agcgtagaga
    32041 aaccaagtac catcgctgat gaagtgccga ccacgcggcg cagctgttgc ggattatcat
    32101 ggtactgggc aacgtatttg gttacaccgt taaagatgcc agctccggca agcacgccga
    32161 gcacggtaat caactggcgg aaatttgccg ccagcccaag ccccgccgga ccaaatgaca
    32221 cagccagcaa cttaccgacc agtaacccgg caccaatctt gaccagtgta ctggccgccg
    32281 tccacaagga cgcttttgcc aacgacatat caggagaagt agttcaacaa agtcgcaatt
    32341 accgtacgct gattgacggg cgacaggttg tagaacagcg gcaggcgcag caggcgctcg
    32401 ctctctttgg tggtgtagcg atcttcaccg tggaactcac caaagcgttc ccccgcaggg
    32461 caaccgtgca gcggaatgta atggaacacc gccatgattt ccgcttcttt cagaaagtta
    32521 atcaacgcgc tccggtcatc aatatcccgc agtttaatgt agaacatatg cgcgttctgc
    32581 acgcagccat cgggaatcga cggcagctcg atacgcccgg ctttcgccag aggcgctaac
    32641 gcatcgtagt agttttgcca cagcgccaga cgttgctggt tgatacgatc cgctgcttcc
    32701 agttgcgccc acaggtacgc agcttgcaga tcggacatca aatagctgga gccaatatcg
    32761 cgccaggtat atttatcgac ctgaccacgg aagaactggc tgcggttagt gcccttttca
    32821 cggatgatct cggctcgttc gattaacgct ttatcgttaa tcagcgtcgc gccgccttca
    32881 ccacccgccg tatagttttt ggtttcatgg aagctaaagc agccaatatg accaatggtt
    32941 cccagtgcac gccctttgta agtggacatc acgccctgag cggcatcttc taccacaaac
    33001 agattatgct ttttcgccaa cgccataatg gtgtccattt cgcaggccac acccgcgtaa
    33061 tggaccggca cgataacgcg cgttttgtcg gtgatcgccg cttcaatcag cgtttcgtcg
    33121 atgttcatgg tgtccgggcg aacatccaca aaaacgattt ttgcgccacg cagcacaaag
    33181 gcattggcgg tggagacaaa ggtgtagctc ggcatgatca cttcatcgcc aggctggata
    33241 tcgagcagca gcgccgccat ctccagcgaa gcggtgcagg acggcgtcag taacactttg
    33301 gcgctgccaa aacgttgctc cagccactgc tggcagcgac gggtaaaacc gccatcgcca
    33361 cacagtttgc cgctacccat tgccgactgc atatagtcga gttcggttcc caccaccggc
    33421 ggtgcgttaa atggaatcat gtgatcacct gtataaccag tacgcggtgc tttctacatt
    33481 cgcaccactt tgtatgtatc gtttaagcgc ggcggtgttg cccatttggg tcgccacccg
    33541 caaagttgtt ttaccgcgag catacgccca gtttagcgcc gtttgcatca gctcagcacc
    33601 tacaccgcgt ccagccagca ggccaattcg cgcatctgtc gcattgagtt cccgtaaaga
    33661 gacataacca cgaataccgc cggacgccgc acgtaaaatc agacattgat gatcaaaggt
    33721 gccgcgcacg gcattttcaa tccactgtgc ataaaagcgt ctgctggcgt caggcgcata
    33781 ccacggcgca cgaaaacggc tttgcgcaaa tgcggcgctg gctaactgac gtaatgcggg
    33841 aatatcggtc tcttgtgcca ctacagcacc actatcactg acattgttca cgggtagtgc
    33901 caaatcaact tcaccttcta ccagggagaa tcccagctgt tgcagggcat ccagttcacc
    33961 cgtatttgat gccgcaattt tggcctgcac ccgtgaccac ggcgctaaca cgtctggcgt
    34021 caggagcggt gcttcagacg taatgcgcac gatggcgctg ttaacaccaa agaaggcgtt
    34081 ttcccaggtt agtggctcaa tactggcgcg gacgggcacg aagtaactcc agcagatatt
    34141 ggccgtagcc agttttcgct aatgaactgg cagcacgctt cacaccctcg tcatcgagcc
    34201 agccgttacg ccaggcgatc tcttccaggc aggcaatctt aaagccctgg cgtttttcta
    34261 ctgtctgcac aaaggtactg gcttcaatca ggctgtcgtg agtgccagtg tccagccagg
    34321 caaatccgcg cccgagcagt tcaacggtca ggttgcccgc ctcgaggtac atctggttga
    34381 tggaggtaat ctccagttca ccacgctccg acggcttcac ctgctttgcg tactccacga
    34441 ctttactgtc gtagaaataa agcccggtca ccgcccagtt tgacttcggc tgttttggct
    34501 tttcttccag cgagatagcg cggaaattgt cgtcaaactc caccacgcca aagcgttccg
    34561 ggtccatcac ctgatagcca aataccgtcg ccccttccgt gcgcgccgca acatgacgca
    34621 gcttcggact gaagccctga ccgaagaaga tgttatcgcc cagcaccaga caagaaggtt
    34681 caccgttgag gaaggtttca ccgatgataa aggcctgcgc cagaccgtcc gggctgggct
    34741 gttcggcgta ttccagctgg ataccgaact cactaccatc gcccagcagg cgctggaaat
    34801 aacctttatc ttccggcgta gtgatgatga gaatttcgcg gataccggcc aacatcagca
    34861 ccgacagcgg atagtaaatc attggcttat cgtaaatcgg caacagttgc ttcgatacgc
    34921 cgcgcgtaat cggatgcaat cgggtgccgg aaccgcccgc caggataata cctttcatgt
    34981 ttgcctccgc tgaaaattag cctttcagac ctaaacgctc gccctgatag ctgccgtcct
    35041 gcacctgctt ccaccagctt tcattagcca gataccactg caccgtttta cgcattccac
    35101 tttcaaaggt ttcctgtggc agccaaccaa gttcacgggc aattttcgaa gcatcaatgg
    35161 cataacgcag atcatgcccc ggacggtcag cgacaaaggt gatcaagtca cgatagtgcg
    35221 ccacgccgtg cggcttgttc ggagccagtt cttccagcag ctcgcaaatg gtttccacaa
    35281 catcgagatt cttacgctcg ttgtgaccac caatattata ggtttcaccg actttcccgg
    35341 tggtcgccac gcaatacagc gcgcgggcgt gatcttccac atacagccag tcacggattt
    35401 gctgcccgtt gccatatacc ggcagcggtt tacccgccag cgcgttgagg atcatcagcg
    35461 ggatcagttt ttccggaaag tggtaaggac cgtagttatt cgagcagttg gtgatcagcg
    35521 tcggcagacc gtaggtccgc agccaggcgc gcaccagatg gtcgctactg gcttttgacg
    35581 cggaataagg gctgctcggc gcatacggcg tggtttcggt gaagaaatca tccgtcgagt
    35641 gcaggtcacc atatacttcg tcggtggaga tatgatgaaa acggaacgct gattttttat
    35701 cttccgtcag cgtattccag taagcccgcg ccgcttcaag caatgtataa gtcccgacaa
    35761 tgttggtttc aataaacgct gccgggccgt caatagaacg gtcaacatgg ctttcggctg
    35821 ccagatgcat gacacagtct ggctgatgct cagtgaatac gcgtgccagt tctgcccgat
    35881 cgcagatatc aactttctca aaggcaaagt gctcgctttg cgcgaccggt gccagcgaca
    35941 tcaggtttcc ggcgtaggtc agcttatcga ccactaccac cgcatcgctc gtttcgttga
    36001 tgatataacg caccagcgcc gagccaataa acccggcacc acctgttatc agaatttttc
    36061 tcatcagcgc cagactcctt tggcatcgac gacatactgc tgatggacat tgtcgccatt
    36121 gataactttg aactgactat gatcgaccag catcaccagc acgtctgccg ttgccagcgc
    36181 ctcgtcaagc tgcgccagag tacaaagccc ggtcagtttt ttcggcaact ggtggatgtt
    36241 aggctcaaca accagcgttt cgccgctgtg ccactgggcg atcagttcag cgatttccat
    36301 cgccgggctt tcgcgcaggt catcaatatt cggtttaaac gccagaccaa agcaggcgat
    36361 tttcagttca ctggcgcgtt tatcggtagc agcaaggcag tcggcaaccg ccgctttcac
    36421 ctgatcgata acccagaacg gtttgtgatc gttaacttcg cgcgcggtac ggataagccg
    36481 cgcctgctgg gggttctgtg ccacgataaa ccacggatca acagcaatgc agtgaccgcc
    36541 cacgccaggg ccaggctgaa gaatattaac gcgagggtga cgattcgcca ggcgaatcag
    36601 ttcccagacg ttaatcccct gatcggcaca aatcagcgac aattcattag caaaagcgat
    36661 attcacatcg cggaagctgt tttcggtgag cttacacatt tccgccgtcc gcgagttagt
    36721 gacgacacac tcaccttcga ggaaaatttt gtacagttcg ctggcgcggg tcgaacaaac
    36781 cggcgtcata ccaccaatca cgcgatcgtt tttaatcagc tcgaccataa cctggcccgg
    36841 taacacgcgt tccgggcagt aagcaatgtt gacgtccgcc tgctcgccca cctgctgtgg
    36901 gaaagtgaga tccggacgca tctctgctaa ccattctgcc atcttctcgg ttgaccccac
    36961 cggcgaggtg gattcaagga tcaccagcgc gccttttttc agcactggcg caatggagcg
    37021 agcagccgat tcaacgtagg tcatatctgg ctcatgatcg cctttaaacg gcgtaggtac
    37081 agcaatcagc caggcatccg cttcaactgg cgtcgtgctc gctcgtaaaa aaccgccttc
    37141 tacggcagtt tttactacgc tcgccaaatc aggttcgacg atatggattt cgccacgatt
    37201 gatggtatca accgcgtgtt ggttgatatc gacaccaatc acctgttttt gccgtgaggc
    37261 aaacgcagct gctgtaggca gcccgatgta acccagtccg ataacagaaa tggtcgcaaa
    37321 actcatagtg atatccgatt attttttaac gcttccagaa tgcgagagca tgcctgacca
    37381 tcaccatacg ggttatgggc gcggctcata gcttgatatt cgttttcgtc tattaaaaga
    37441 cgcgtcactt cctcgacaat tcgctgctta tccgtgccta ccagacgcac cgtacccgcc
    37501 gtcaccgctt ccggacgctc agtggtatcg cgcatcacca gcaccggttt ccccagcgaa
    37561 ggcgcttctt cctgaatgcc gcctgagtca gtcaaaatca gccaggcgtg gttcatcagc
    37621 cagacaaacg gtaaatactc ctggggatcg atcagaatga catttttcac atgccccaga
    37681 atgcgattga ccggctctct gacgttcggg ttgagatgca ccggatagac aatctggatg
    37741 tcctggtgcg tggtggcgat gtctgccagc gcgtggcaga tttcttcaaa gccacgaccg
    37801 aaactttcac gcctgtgacc ggtcaccaga atcatctttt tatcggggtc gataaacggg
    37861 taatttgccg ccagttctga acgcagcgtg tcgctgctca tcacctgatc acgcacccat
    37921 aacagtgcat caatgactgt attaccagta atgaagattc ggctatccgc aacgttttca
    37981 cgcagcaagt tttgccggga agtttcggtt ggagagaagt gatacatcgc cagatgcccg
    38041 gtcaatgtac ggttagcctc ttccggccac ggcgaataga gatcgcccgt gcgtaaacca
    38101 gcctcaacgt gaccaacagg aatacgctga taaaacgccg ccaggctggt tgccagcgtc
    38161 gttgttgtgt cgccgtgaac cagcacgacg tctggtttga actcggcaag aataggtttt
    38221 agcccttcca gaatccgaca ggttatctct gtcaggccct gtcctggctg cattatgttg
    38281 agatcgtagt caggtacaat ggaaaagagt ttcagcacct gatcgagcat ctcccgatgc
    38341 tgcgcagtga cgcaaacttt agcctcaaaa aaaggatctt ttgccaacgc atgcaccaac
    38401 ggagccatct tgatggcttc cgggcgcgta ccaaatacag tcagtacttt cacatcgatt
    38461 ctcttcgaat aagcggcgag cgcctttgcg ctcgcagcag cagtgttgct atttcgagca
    38521 acggcgggtt aatgcgacac cagccccgat cagccccccg acaatgcccc acataatcat
    38581 caggaaggca cgacgtgggc tatcgcgttt taccggttct tccggcgtac gcaaatagcg
    38641 ataggtctga aaacgcggat ccagagttgg accaacattc agggtgttta acatggcccg
    38701 attctgatca tagtcgagat caaaggccgg accgacggcc tgtaaatttt ccagtcgagc
    38761 ctggagcatt ggacgcccaa gcaggaacat ttctgaatca ggtaattcct cggcaggcac
    38821 atctgtcgca ctgcgcgaaa tattatgctg ctcagcaatt ttcagcgcct gctcaatgct
    38881 gttcatccgg cggtcgtaga tggctttcgc cacctcttcc tgacgcttca cctgagcttt
    38941 catctggatg gtgcgtgccg cccatgcgcc tttcagctca tcattcagat ggctggctgc
    39001 acgctggctg gcaaaagcaa catactgacg taacaggtta ttagcgtcag gcgcggtttc
    39061 ggcaataagc ttcacgctgt cattgaccgc gcgggtaaag tctccgggga taaactggat
    39121 gttgttaatc atttcatcca gcaacgccgc atcggctttg ctgttgccca ccatccgctg
    39181 tttgtaatag tcggtttgca gccagaactc tctgcgggta tcccacgagg ccagttgcat
    39241 aacaaactct ttataggctt cgtccatgac cgatggttga tcggcagaag ccatgtttga
    39301 acggacatcc aggttacgca aaa
//
LOCUS       DAEVZA010000031        37959 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEVZA010000031 DAEVZA010000000
VERSION     DAEVZA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.031"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..144
                     /gene="paaD"
                     /locus_tag="LAF92_001664"
     CDS             <1..144
                     /gene="paaD"
                     /locus_tag="LAF92_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HBI9790685.1"
                     /translation="AHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFDYF
                     KCI"
     gene            152..1222
                     /gene="paaK"
                     /locus_tag="LAF92_001665"
     CDS             152..1222
                     /gene="paaK"
                     /locus_tag="LAF92_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HBI9790686.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            1219..1986
                     /locus_tag="LAF92_001666"
     CDS             1219..1986
                     /locus_tag="LAF92_001666"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HBI9790687.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            1986..2774
                     /gene="paaB"
                     /locus_tag="LAF92_001667"
     CDS             1986..2774
                     /gene="paaB"
                     /locus_tag="LAF92_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HBI9790688.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            2776..4203
                     /gene="paaC"
                     /locus_tag="LAF92_001668"
     CDS             2776..4203
                     /gene="paaC"
                     /locus_tag="LAF92_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HBI9790689.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     TRLARPVVVIDKMAGKVVTIAAAAVNPDSATHKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            4193..4615
                     /gene="paaI"
                     /locus_tag="LAF92_001669"
     CDS             4193..4615
                     /gene="paaI"
                     /locus_tag="LAF92_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HBI9790690.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTVQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTIIGEA"
     gene            4615..5820
                     /gene="paaJ"
                     /locus_tag="LAF92_001670"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             4615..5820
                     /gene="paaJ"
                     /locus_tag="LAF92_001670"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HBI9790691.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            5847..7160
                     /gene="paaF"
                     /locus_tag="LAF92_001671"
     CDS             5847..7160
                     /gene="paaF"
                     /locus_tag="LAF92_001671"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HBI9790692.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            7261..8211
                     /gene="paaX"
                     /locus_tag="LAF92_001672"
     CDS             7261..8211
                     /gene="paaX"
                     /locus_tag="LAF92_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HBI9790693.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKETAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            8193..8783
                     /gene="paaY"
                     /locus_tag="LAF92_001673"
     CDS             8193..8783
                     /gene="paaY"
                     /locus_tag="LAF92_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HBI9790694.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKLTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            9013..9873
                     /gene="pdxI"
                     /locus_tag="LAF92_001674"
     CDS             9013..9873
                     /gene="pdxI"
                     /locus_tag="LAF92_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HBI9790695.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVIMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            9937..12243
                     /locus_tag="LAF92_001675"
     CDS             9937..12243
                     /locus_tag="LAF92_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HBI9790696.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDSYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            12414..13019
                     /locus_tag="LAF92_001676"
     CDS             12414..13019
                     /locus_tag="LAF92_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBI9790697.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECDQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNALLVILMLFCTILTEFCGLLAQTINGIRSFAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLIWTAINRCRSVLLMSAER"
     gene            13019..13915
                     /locus_tag="LAF92_001677"
     CDS             13019..13915
                     /locus_tag="LAF92_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBI9790698.1"
                     /translation="MLEKSLATLFALLILATLINRFLLWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATGQVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            13931..15675
                     /locus_tag="LAF92_001678"
                     /pseudo
     CDS             13931..15675
                     /locus_tag="LAF92_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
     gene            15665..16981
                     /locus_tag="LAF92_001679"
     CDS             15665..16981
                     /locus_tag="LAF92_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HBI9790699.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(17032..17637)
                     /gene="azoR"
                     /locus_tag="LAF92_001680"
     CDS             complement(17032..17637)
                     /gene="azoR"
                     /locus_tag="LAF92_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBI9790700.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            17838..21740
                     /gene="hrpA"
                     /locus_tag="LAF92_001681"
     CDS             17838..21740
                     /gene="hrpA"
                     /locus_tag="LAF92_001681"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBI9790701.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            22012..22812
                     /locus_tag="LAF92_001682"
     CDS             22012..22812
                     /locus_tag="LAF92_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBI9790702.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            23009..24448
                     /gene="aldA"
                     /locus_tag="LAF92_001683"
     CDS             23009..24448
                     /gene="aldA"
                     /locus_tag="LAF92_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBI9790703.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(24490..25491)
                     /gene="gap"
                     /locus_tag="LAF92_001684"
     CDS             complement(24490..25491)
                     /gene="gap"
                     /locus_tag="LAF92_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBI9790704.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            25680..26210
                     /gene="cybB"
                     /locus_tag="LAF92_001685"
     CDS             25680..26210
                     /gene="cybB"
                     /locus_tag="LAF92_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBI9790705.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            26455..26628
                     /locus_tag="LAF92_001686"
     CDS             26455..26628
                     /locus_tag="LAF92_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790706.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(26700..26849)
                     /gene="hokB"
                     /locus_tag="LAF92_001687"
     CDS             complement(26700..26849)
                     /gene="hokB"
                     /locus_tag="LAF92_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBI9790707.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            27248..28888
                     /gene="trg"
                     /locus_tag="LAF92_001688"
     CDS             27248..28888
                     /gene="trg"
                     /locus_tag="LAF92_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBI9790708.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(28926..29849)
                     /locus_tag="LAF92_001689"
     CDS             complement(28926..29849)
                     /locus_tag="LAF92_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9790709.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGSQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            30066..31409
                     /locus_tag="LAF92_001690"
     CDS             30066..31409
                     /locus_tag="LAF92_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI9790710.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            31634..33289
                     /gene="opgD"
                     /locus_tag="LAF92_001691"
                     /gene_synonym="mdoD"
     CDS             31634..33289
                     /gene="opgD"
                     /locus_tag="LAF92_001691"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBI9790711.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            33429..33653
                     /locus_tag="LAF92_001692"
     CDS             33429..33653
                     /locus_tag="LAF92_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBI9790712.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            33716..34252
                     /gene="rimL"
                     /locus_tag="LAF92_001693"
     CDS             33716..34252
                     /gene="rimL"
                     /locus_tag="LAF92_001693"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBI9790713.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(34247..35227)
                     /locus_tag="LAF92_001694"
     CDS             complement(34247..35227)
                     /locus_tag="LAF92_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBI9790714.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            35351..36343
                     /gene="tehA"
                     /locus_tag="LAF92_001695"
     CDS             35351..36343
                     /gene="tehA"
                     /locus_tag="LAF92_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBI9790715.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQFAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            36340..36933
                     /gene="tehB"
                     /locus_tag="LAF92_001696"
     CDS             36340..36933
                     /gene="tehB"
                     /locus_tag="LAF92_001696"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBI9790716.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            37236..37904
                     /locus_tag="LAF92_001697"
     CDS             37236..37904
                     /locus_tag="LAF92_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBI9790717.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         9384 a         8813 c        10054 g         9708 t
ORIGIN      
        1 gcccatttgc cgccagaagt acgttgcccg cgctgcgcca gcgtccatac cacacttatc
       61 agtgaatttg gttccacggc ctgcaaagca ttgtaccgct gcgatagttg ccgcgaacct
      121 ttcgattatt tcaaatgtat ttgaggatgc catgacaacg tttcattcct taacagtggc
      181 aaaagtcgag ccggaaaccc gtgatgcggt gaccattacc tttgcggtgc cccagccttt
      241 gcaggaggcg tatcgctttc gccccggtca acatttgacc ttaaaagcca gtcttgatgg
      301 tgaagaatta cgccgttgtt actccatttg ccgcagctac ctgcctggcg aaattagtgt
      361 ggcggtgaaa gccattgaag gcggacgttt ctcccgctat gcccgcgaac acatccgcca
      421 gggtatgacg ctggaggtca tggtgccgca ggggcatttc ggctatcagc cacaggccga
      481 acgccagggc cgctatctgg ctattgcagc aggatcaggt attacgccaa tgctggcgat
      541 tatcgccacc actttacaaa ccgagcctga aagtcagttc accctgatct acggtaaccg
      601 taccagccag agcatgatgt ttcgccaggc actggcagac ctgaaagaca aatatcctca
      661 gcgtttacag ttgttgtgca ttttcagtca ggaaaccctc gacagcgatc tgcttcacgg
      721 gcgtattgac ggtgaaaaat tacagtcact tggggcctcg ctcattaatt ttcgtcttta
      781 tgatgaggca tttatttgtg gtccggcggc gatgatggat gagacggaag ccgccttaaa
      841 agcactggga atgccagata aaaccattca tctggagcgg tttaatacgc ctggcacgcg
      901 cgtcaaacgt agcgttaacg tgcaaagtga cggacaaaaa gtgactgtac gtcaggatgg
      961 gcgggatcgg gaaatcgtgc ttaatgccga cgatgaaagc attctcgatg cggcattgcg
     1021 ccagggggct gatctgccct atgcctgcaa aggcggcgtc tgtgcgacct gcaaatgcaa
     1081 agtgctgcgt ggcaaagtgg cgatggaaac caattacagt ctggaaccgg atgaactggc
     1141 cgcaggttat gtgttgagtt gccaggcact gccgctgacc agcgatgtgg tggttgactt
     1201 tgacgcgaag gggatggcat gagcgaactg atcgtcagcc gtcagcaaca agtattgttg
     1261 ctgaccctta accgtcccgc cgcacgtaat gcgctaaata atgcccttct gacgcaactg
     1321 gtaaatgaac tggaagctgc ggctaccgat agcagcattt cggtctgtgt gattaccggt
     1381 aatgcacgct tttttgccgc tggggccgat ctcaacgaaa tggcagaaaa agatctcgcg
     1441 gccaccttaa acgatacacg tccgcagcta tgggcgcgat tgcaggcctt taataaacca
     1501 cttatcgcag ccgtcaatgg ttacgcgctt ggggcgggtt gcgaactggc attgttgtgc
     1561 gatgtggtgg ttgccggaga gaacgcgcgt tttgggttgc cggaaatcac tctcggcatc
     1621 atgccaggcg caggaggaac gcaacgttta atccgtagtg tcggtaaatc gttagccagc
     1681 aaaatggtgc tgagcggaga aagtatcacc gctcagcaag cacagcaggc cgggctggtt
     1741 agcgacgtct tccccagcga tttaaccctc gaatacgcct tacagctggc atcgaaaatg
     1801 gcacgtcact cgccgctggc cttacaagcg gcaaagcaag cgctgcgcca gtcgcaggaa
     1861 gtggctttgc aagccggact tgcccaggag cgacagttat tcaccttgct ggcggcaaca
     1921 gaagatcgtc atgaaggcat ctccgctttc ttacaaaaac gcacgcccga ctttaaagga
     1981 cgctaatgat ggaattcatc ctcagtcatg tagaaaaggg cgtgatgaca ctaacgctca
     2041 accgcccgga acgcctgaac agttttaatg atgagatgca cgcacaactg gcagagtgcc
     2101 tgaaacaggt cgagcgcgac gacactatcc gttgcctgtt acttacaggt gccgggcgcg
     2161 gattttgtgc tggtcaggat cttaacgatc gtaacgttga tcccacaggc cccgcaccgg
     2221 atttagggat gtcagttgaa cgtttctata acccactggt acgacgcctg gcaaaactgc
     2281 caaaaccggt gatctgtgca gtcaatggcg tggcggcagg ggcaggcgca acactggcac
     2341 tggggtgcga catcgttatt gctgcccgtt cagcaaaatt cgtcatggcg tttagtaagt
     2401 taggcttaat acccgattgc ggtggaacct ggttactgcc acgcgttgct gggcgagcgc
     2461 gcgccatggg gctggcactg ctggggaatc aactgagtgc tgaacaggcg cacgaatggg
     2521 ggatgatctg gcaggttgtt gatgatgaaa cgctggcaga taccgcgcaa cagctggcac
     2581 ggcatctggc gacacaaccg acatttggtc ttggacttat caagcaagcg ataaatagcg
     2641 ctgaaaccaa tacgctcgat acgcaactgg atctggaacg tgactatcag cgacttgccg
     2701 gacggagcgc agattatcgt gaaggtgtca gtgcgttcct ggctaaacgc tcaccgcagt
     2761 tcacggggaa atagcatgat gataaatgtg caaactgtgg cagtgattgg gagcggcacc
     2821 atgggggcag gcattgctga agttgccgcc agtcatggac atcaggtttt actgtatgac
     2881 atttctgctg aagcgctgac ccgcgcaatc gacgggatac acgcgcggct aaattcacgc
     2941 gtgacgcggg gaaaactgac cgctgaaacc tgtgaacgca cattgaaacg cctgatcccg
     3001 gtgaccgata ttcacgcgct ggcagcagcg aatctggtta ttgaagcggc gtctgaacgt
     3061 ctggaagtta aaaaagcgct ctttgcacaa ctggcggaag tttgcccgcc acaaacgcta
     3121 ttgaccacta acacttcgtc aatctctata accgcgattg ctgcggaggt aaaaaatcct
     3181 gaacgtgttg cggggctgca tttttttaac ccggcaccgg tgatgaagtt ggtggaggtg
     3241 gtcagtgggc tggcaacggc agcggaagtt gtcgagcagt tgtgtgaact gacattgagt
     3301 tggggtaagc agccagtgcg ctgtcattcg acccccggat ttatcgttaa ccgtgttgcg
     3361 cgtccttatt attccgaggc ctggcgggca ctggaagagc aggttgctgc accagaagtg
     3421 attgacgctg cacttcgcga tggcgctggt ttcccgatgg ggccgctgga attaaccgat
     3481 ctgattggtc aggacgtcaa ttttgctgtc acctgttcgg tgtttaacgc tttctggcag
     3541 gagcgtcgtt ttttaccttc gctggtgcaa caggaactgg tgattggtgg acggttgggc
     3601 aagaaaagtg ggctgggcgt gtacgactgg cgcgcggaac gtgaggcagt tgttggcctg
     3661 gaagcggtaa gcgacagttt tagcccaatg aaagtagaaa agaaaagtga cggtgtcacg
     3721 gaaattgacg atgttttatt gattgagaca caaggcgaga cggcacaggc gctggcaaca
     3781 cgactggcgc gcccggtggt agttatcgac aaaatggcgg gcaaggtggt gaccattgct
     3841 gcggcagcgg tgaacccgga ctcagcgacc cacaaggcca tttattacct gcaacagcag
     3901 ggcaaaacag tgctgcaaat tgcagattac ccaggaatgc tgatttggcg aacggtagca
     3961 atgatcatca atgaagccct tgatgcgctg caaaaaggcg tggcctctga acaggatatc
     4021 gataccgcca tgcgtcttgg ggtgaattat ccatatggcc cgcttgcctg gggagcgcaa
     4081 cttggctggc agcgaatatt aaggctcctg gaaaatctac aacatcacta tggcgaagaa
     4141 cgctatcgcc catgttcatt gctgcgccaa cgggcgcttc tggagagcgg ttatgagtca
     4201 taaggcctgg caaaatgccc atgcaatgta tgagaacgat gcctgcgcga aagcgcttgg
     4261 catcgacatt atctcaatgg atgaaggctt tgctgtagtg accatgaccg tcactgtaca
     4321 aatgcttaat ggtcatcaaa gttgccacgg cgggcagcta ttttcactgg ctgatactgc
     4381 ctttgcctac gcctgcaata gccaggggtt ggcagctgtc gcttctgcct gcacgattga
     4441 ttttttacgt ccaggctttg ccggagacac cttaactgcg actgcgcagg ttcgtcatca
     4501 gggcaagcaa accggtgttt acgacatcga aattgttaac caacaacaaa aaacggttgc
     4561 gctgtttcgc ggtaaatctc accgcatcgg cggcaccatt ataggagaag cctgatgcgt
     4621 gaagccttta tttgtgacgg aattcgtacg ccaattggtc gctacggcgg ggcattatca
     4681 ggtgttcggg ctgatgatct ggctgctatc cctttgcggg aactgctggt gcgaaacccg
     4741 cgtctcgatg cggagtgtat cgatgatgtg atcctcggct gtgctaatca ggcgggagaa
     4801 gataaccgta acgtagcccg gatggcgact ttactggcgg ggctgccgca gagtgtttcc
     4861 ggcacaacca ttaaccgctt gtgtggttcc gggctggacg cactggggtt cgccgcacgg
     4921 gcgattaaag cgggcgatgg cgatttgctg atcgctggtg gcgtggagtc aatgtctcgg
     4981 gcaccgtttg ttatgggcaa ggcaaccagt gcattttctc gtcaggctga gatgtttgat
     5041 accactattg gctggcgatt tgtgaacccg ctcatggctc aacaatttgg aactgacagc
     5101 atgccggaaa cggcagagaa tgtagctgaa ctgttaaaaa tctcgcgaga agatcaagat
     5161 agttttgcgc tacgcagtca gcaacgtacg gcaaaagcgc aatcctcagg cattctggct
     5221 gaggagattg ttccggttgt gttgaaaaac aagaaaggcg ttgtaacaga aatacaacat
     5281 gatgagcatc tgcgcccgga aacgacgctg gaacagttac gtgggttaaa agcaccgttt
     5341 cgtgccaatg gggtgattac cgcaggcaat gcctccgggg tgaatgacgg agccgctgcg
     5401 ttgattattg ccagtgaaca gatggcagca gcgcaaggac tgacgccgcg ggcgcgtatc
     5461 gtagccatgg caaccgccgg ggtggaaccg cgtctgatgg ggcttggtcc ggtgcctgca
     5521 actcgccggg tgctggaacg cgcggggctg agtattcacg atatggacgt gattgaattg
     5581 aacgaagcgt tcgcggccca ggcgttgggt gtactacgcg aattggggct gcctgatgat
     5641 gccccacatg ttaaccccaa cggaggcgct atcgccttag gccatccgtt gggaatgagt
     5701 ggtgcccgcc tggcactggc tgccagccat gagctgcatc ggcgtaacgg tcgttacgca
     5761 ttatgcacca tgtgcatcgg tgtcggtcag ggcatcgcca tgattctgga gcgtgtttga
     5821 gcatatcaac ctgcgagtac cctacaatga taaccaatac aaagcttgac ccgattgaaa
     5881 ccgcgtctgt tgatgagtta caggcgttgc aaacacagcg tctgaaatgg acgctcaaac
     5941 acgcgtatga aaatgtcccg atgtatcggc gcaaattcga cgcagcaggc gtacatcctg
     6001 atgatttcag ggaactttca gacctgcgta aatttccctg taccaccaaa caggatctgc
     6061 gcgacaacta tccctttgac acctttgccg tgccgatgga acaagtggtg cgcattcatg
     6121 cttcttcggg aaccacaggt aaaccgacag ttgtcggtta tacgcaaaac gatattgata
     6181 actgggccaa tattgtagcg cgttctttgc gtgcggcagg gggctcgccc aaagacaaaa
     6241 ttcatgttgc ctatggttac ggcctgttta ctggtgggct gggcgcgcac tatggtgccg
     6301 aacgtctggg cgctacggtg atcccgatgt ctggcgggca gacggaaaaa caagcgcaac
     6361 tgatccgcga ttttcaacca gatatgatca tggttacgcc atcttattgc cttaacctga
     6421 ttgaagagct ggagcggcag ttgggcggtg atgccagcgg ttgctcgctg cgggttggcg
     6481 tgtttggtgc tgagccgtgg acacaggcca tgcgtaaaga gattgaacgg cgtctgggga
     6541 tcaccgcact ggatatttat ggcctgtcag aagtgatggg gccgggggtg gcgatggagt
     6601 gtctggaaac taccgacggt ccaactattt gggaagatca tttctatcct gaaattgtta
     6661 atcctcatga cggcacaccg cttgccgatg gtgaacatgg cgaactgtta ttcaccacgc
     6721 tgaccaaaga agcattgccg gtcattcgtt accgcacgcg tgatttaacc cgactgttac
     6781 caggaacggc gcggactatg cgccgcatgg atcgcatcag cggacgcagc gatgacatgt
     6841 tgattattcg cggtgtaaat gtctttccgt cacaactgga agaagagatt gtcaaattcg
     6901 aacatttatc gccgcattac caactggagg tgaaccgccg ggggcatctt gattcacttt
     6961 ctgtgaaagt ggagttgaaa gaaagtagcc tgacattgac gcatgagcag cgttgccagg
     7021 tatgccacca gctgcgccat cggattaagt cgatggtggg gatctctacc gatgtgatga
     7081 tcgttaactg tggcagtatc ccgcgttcag aaggcaaggc gtgtcgggtg tttgatctgc
     7141 gcaatattgt tggtgcctga cgtgtcgtct ggccctggtg aggtaaagcg tcagggccag
     7201 aagtcgatac gacctgtgct atgattcata aatcacaaca ataacaacag actgaatcga
     7261 atgagtaaac ttgatacttt tatccaacat gctgtaaacg ctgttccggt cagtggcaca
     7321 tctttgatct cctctctgta tggtgattcg ctttcccatc gtggtggtga aatctggctt
     7381 ggtagtctgg ctgctttgct ggaagggctg ggatttggtg agcgtttcgt gcgcactgct
     7441 ttgtttcgtc ttaataaaga aggctggctg gatgtttccc gcatcgggcg acgcagtttc
     7501 tatagcctca gtgataaagg cttgcgcctg acgcgacggg cagaaagtaa aatttatcgc
     7561 gcagagcaac ctgcatggga tggtaaatgg ctcctgttgc tctcggaagg tttagataaa
     7621 gcgacgctgg ctgatgtcaa aaagcagttg atctggcaag gttttggcgc actggcaccc
     7681 agcctgatgg catcgccgtc gcaaaaactg gcggatgtac agacactttt gcatgaagcg
     7741 ggggtggcgg ataacgtgat ttgttttgaa gcgcaaatac cactggcgct ttctcgcgca
     7801 gcactgcgtg ccagagtaga agagtgctgg catttaactg aacaaaatgc catgtacgaa
     7861 acctttattc agtcattccg cccgctggtg ccgctgttaa aagagacggc agacgagtta
     7921 accccggagc gtgcatttca tattcagctt ttactgatcc atttttatcg ccgtgtcgtc
     7981 cttaaagacc cattgttgcc ggaggagttg cttccggctc actgggcagg gcatacggcg
     8041 cgtcagctgt gtatcaatat ttatcagcgc gtagcgcctg ctgctttagc gttcgttagt
     8101 gaaaaaggtg aaacctcagt cggtgaactg cctgcgccgg gaagcctgta ttttcaacgt
     8161 tttggcggct tgaatattga acaggaggcg ttatgccaat ttaccagata gatggtctga
     8221 ctccggttgt gccagaagag agctttgtcc atccgacagc ggtattgatc ggcgatgtta
     8281 ttctcggcaa gggcgtttac gttgggccta atgccagcct gcgtggcgat tttggtcgta
     8341 tcgtggtgaa agatggcgcg aacattcagg ataattgcgt tatgcacggt tttcccgagc
     8401 aggatactgt cgtggaagaa gatgggcata ttggtcatag cgctatcctt cacggctgca
     8461 ttatccgccg caatgcatta gtgggaatga acgcggtagt gatggacggt gcggtgattg
     8521 gcgagaacag cattgttggc gcatccgcat ttgtgaaagc caaagcagaa atgcctgcta
     8581 attacctgat tgtcggcagc ccggccaaag cgattcgtga acttagtgag caggagttgg
     8641 catggaaaaa gcagggtacg catgagtacc aggtgctggt gacacgctgt aagctgacgt
     8701 tgcatcaagt cgaaccattg cgggaagttg aacctggcag gcagcgcctg gtatttgatg
     8761 agaatctgcg accgaaacag taacgtccag atactgaaca ttctaatttt tagcatgtga
     8821 tacctaaacc gcaacgctga tacaggttgc ggttttttat tgccggatgc ggtacttgtc
     8881 gcgttttgtt ttgtgttttt caggacaata gagcaactca tccagtaatc ttgtttacgc
     8941 cctcgcatta tttatctctt ttcgtttcta tactgatttt tcttaatccg ttttattaca
     9001 gggcagggtg cgatgagcag caatacattt actctcggta caaaatccgt taaccgtctt
     9061 ggttatggcg cgatgcaact ggcaggtcct ggggtttttg gccccccaag agatcgccac
     9121 gtcgctataa ccgtgctgcg cgaggcgctg gcattgggcg tcaatcacat tgataccagc
     9181 gacttttatg gtccgcacgt caccaatcag attatccgcg aagcgcttta tccttactct
     9241 gacgacctga caattgtcac taaaattggt gcgcggcgtg gagaggacgc ttcctggttg
     9301 cccgcctttt ctccggcaga gttgcaaaaa gcggtgcacg ataatctacg taatctcggc
     9361 ctggacgtac tggatgtggt taacctgcgc gttattatgg gggatggtca tggcccagcg
     9421 gaaggatcga ttgaggccag cctgaccgtg ctggcagaga tgcaacaaca aggcttggta
     9481 aaacatattg gcctgagcaa cgtcacaccg acgcaggttg cagaggcgcg caagattgcc
     9541 gaaattgtct gtgtgcaaaa cgaatacaac atcgcgcacc gtgctgatga tgcaatgatt
     9601 gatgctttgg cccacgatgg cattgcctac gtgccgttct tcccgctcgg gggctttaca
     9661 ccgctgcaat cctctacgct gtcggatgtt gctgcgagcc tgggtgcaac acccatgcag
     9721 gtggcgctgg cgtggctgtt acaacgttca ccgaatattt tgctgatccc agggacgtct
     9781 tctgttgcgc atttacggga gaatatggct gctgaaaaat tgcatctttc tgaggaagtg
     9841 ttgtctacgt tggatggtat ttcgcgagaa taacgaatat acaaaaggga aagatgcatt
     9901 tcccttttgt ttctttttta atggcatgga gtgcatatgt ttaaggccag gatttgcggc
     9961 tggataggtt tacttcccct gtttatgctg agtcttccag tacaggcgga acttcgatgt
    10021 gttgcaaata cagttgatat tgaaccattt ttttctgcgg ctaccgctga agataaacaa
    10081 caagttgaac aagctatcaa cagtagcgtg aatcttgtcc ccttcggttt atctgcatcg
    10141 gactggaaag tgcatcgtgg cgatttagtg gtagaaggta atatagagag taatcaaaaa
    10201 ttgattgttc ttggtaattt gacagtcaaa ggtaatattt ccactttctc tctttctaat
    10261 ccatgggtta ttctcggtaa cgtgacggca acgaatatag ttacggattc accactgtta
    10321 attaccggtt cgataaatgc aagtggactg gtatttatcg actcatatta cgataatccg
    10381 tctacgatta aggggagtat taatgcgcgt gggatattta tcaatgacat aattgcacct
    10441 gttgttgcgt cttcgacaaa tagtgaattc atggtccgtg cgagtgacaa aaatgacact
    10501 gaaaatgtca aaaaagcgct gatgataata aatcctgatg catattattg gggtctaata
    10561 aatgatgaag atgctctgaa agaaattttt aagcgaagca atattcgcat ggcagggaat
    10621 gtctgtaatc agatgaaaaa agaagcgctg tttcgcccta agccttctcc tgagttagtg
    10681 caggaattgc aaatgctgga tgaaggcaac gttgctgcat ttgaaggacg agatattgcg
    10741 acatttgatc ttgccataat gcgaactctc ccaaggctta aaggaatttc agctaaccta
    10801 cgcaaacaac ttattaatag caatgatgaa caaacgattg aaagtatggc gagatatatg
    10861 cctgacaatg agattctgga gttgaccgat cagcaattgg gttaccagcc tgttgttctg
    10921 gggttgctcg accgtgaacc gctctctgtc gaaataatga cgcgaatgag tcgtctacct
    10981 gatggtgtcg gtccgttgaa tcttgcgcta cgtgaaaatc tccctctgga tatcgtaatg
    11041 actctggcga aaagagattg ggatatgatc attcaggagc tttataaaga tgcgtggtta
    11101 ttgcctgaat ccattattga tagctatatc cgcagtgatg attcctctat tcgtcaggtc
    11161 ggtgctggag gacaactcac ctacaatcag gcaatgcagt tggcgaacga ttcatcaaac
    11221 aatgttgtca caagcttagc gttcaagctg gcagagatga aacaccatgg tcaattattg
    11281 cggatgacgc cacaagagag tgacaaagtt gcagcctatt tataccaaaa attcgagaat
    11341 gacgatgatc taatacgtgt gttattttta gcattgccag ataacttaca gtttaatttt
    11401 gttaaaagga tggagaaaaa atcccctgcc tacttttgct gtcgggatat gcaggtaatt
    11461 cactctgacg ctgctctaca acggttattg acacgtttca acgatcctga ggggtggagt
    11521 aatctggcga aaaatcagta tctgagcacg tcgatgaaac aaaaaatttg gcaacgtgct
    11581 ttatcgcatc ggaaaaataa tccgaaagca gattcagctg cgtatgaaac cagcgctgat
    11641 atgattttgt ctgagcttat cagccacggt gaagttgatg atcagatgct attaaatgcc
    11701 accgccctga tacgtttgga agactgggac tttttagaga gtgcattagt tagttgggat
    11761 aatttacccg ctgttgtcct caaggaatta cagcaaaata cgccacgcaa tgatatttgg
    11821 gcgaagtttt ttctgaggca ggaaaacagc tcccgtgcgc aggttgatga agcattacgc
    11881 gtctattatg cactcgaccc cgatgcgtta gcacaactgg atgtactggc aaaacaaccg
    11941 gatcgtatat ggtggagtac actggcgaaa agcaatctca catttttcaa gttcggcgca
    12001 cttaacaacc gccacacacc gcctgcagta ctggcggcag aaattgatcc cgagtggtgg
    12061 attgtggcga tgaataatcc ccgttttcca gttgatgtat taaaggcgag gctgaaacgt
    12121 gatcctttgc tggcgttaga gcttgttaac cctgaactag atttagtccg ccagctggcg
    12181 cttaacggta agacgcgcgc aatacgggaa caagcgatga gaaaacttga tgagttgtat
    12241 tgatccacaa cgtaatgttt cttaactatt tgattaattg gggataatca ttcctggcag
    12301 tgagtcccca ataccttgat atattctgaa tttttaatga agcggcgtgt tgcgatatct
    12361 ccgtcagggg aattgatgca ccatagcgca aaccgaatta tcaaggattg ataatgacgc
    12421 tctaccagat aaaaccgctc tttcagtcgc tgttaaggcc gacgatgttt tggctttata
    12481 agcaccacgt tacagcgaat cacatcactc ttgctgcact ggcgctttct cttcttaccg
    12541 gattgctgtt agtgttggtg gcacaaccca tcctcttttt gctattgccc atcgtgcttt
    12601 ttatccgtat ggcgctcaat gcactggatg gcatgttggc gcgtgagtgc gatcagcaaa
    12661 cacgtctggg ggcgattttg aatgaaactg gcgatgttat ttccgatatt gcgctctatt
    12721 taccgttttt atttttaccg gaaagtaacg cattactcgt aatactcatg ttattttgca
    12781 ccatattgac tgagttttgc ggtttactcg cgcagacgat taacggtatt cgcagttttg
    12841 ccggtccatt tggcaaaagc gatcgcgcgt taatatttgg tctgtggggg cttgccgttg
    12901 ccatttatcc acagtggatg caatggaata atcttttatg gagcattgct tcaattctgc
    12961 ttatctggac tgcgattaat cgttgtcgga gtgtgcttct tatgagcgct gaaagataat
    13021 gctggaaaaa tctctggcaa cacttttcgc gttgttaatt ttagcaacgc tgataaaccg
    13081 ttttctgcta tggcggttac cggagagaaa aggggatgaa gttacattac gtattcgtac
    13141 ctggtggggt atcgtcattt gtttttcact ggtgatttca ggtccacgct ggatgacgtt
    13201 gacgtttttt gcgctgataa gttttctggc attgaaagaa tattgtacgc ttatatccat
    13261 acattttccg cgttggctat attgggttat tcctcttaac tatttgctta tcggttttaa
    13321 ttgctttgag ctgtttctgt tatttatacc tttggctggg tttctaatat tagccaccgg
    13381 gcaagttttt gtgggggacc cctccggttt tctgcatacc gtgagcgcca ttttttgggg
    13441 ctggataatg accgttttcg ccttgagtca tgccgcctgg ttattaatgt tgccaaccac
    13501 aaatatccag ggcggggcgt tactggtgtt atttcttttg gcattaaccg agtcaaacga
    13561 tattgcacag tatttatggg ggaaatcctg cggcagaaga aaagtggtcc ccaaagtcag
    13621 cccgggaaaa acgctggaag gtctggtggg gggcgtaatc accaccatga ttgcgtcact
    13681 gattattgga ccgttgctga caccgctaaa tacattacag gcattattag cgggtttgtt
    13741 aattggtatt agtggttttt gcggcgatgt cgtgatgtca gccatcaaac gagatattgg
    13801 tgttaaagat agtgggaaac tattaccagg acatggcgga ctccttgaca ggattgactc
    13861 attaattttc accgctccgg tattttttta ttttattcgc tactgctgtt actgaaggaa
    13921 aatagagaaa atggaaaatt cacgcatccc tggggaacat ttttttacca gcagtgataa
    13981 tacagcgttg ttttatcggc actggcccgc tttacagccc ggggctaaaa aggtcatcgt
    14041 cttatttcat cgcggtcatg aacattctgg tcgtctacaa catctcgttg atgaactggc
    14101 gatgccagat actgtttttt atgcctggga tgcccgaggg catggacaaa cttcggggcc
    14161 gcgtggttat agcccatctc ttgcgcgttc agtgcaggat gtcgatgaat ttgttcgttt
    14221 tgctgccagc gatagccagg tcggactgga tgaggtggta gtgatcgcgc aaagcgtcgg
    14281 cgcagtgctg gttgccacat gggttcatga ttatgcgcct gcaattcgcg ggctggtgct
    14341 ggcttctccg gcctttaagg ttaaattgta tgtaccgctg gcacgtcctg cgctggcgtt
    14401 atggcatcgt ctgcgtggtc tgttttttat taattcctat gtgaaaggac gctatttgac
    14461 ccacgatcgg caacgggtgg cgagtttcaa taatgatccg ctgatcacac gggcgattgc
    14521 cgttaatatc ttgctcgatc tctacaaaac gtctgaacgt attattagag atgcggcggc
    14581 gattacgctc cccacgcaac ttctgatatc aggcgatgac tatgtggtgc atcgccaacc
    14641 gcagattgat ttttatcaga gattacgtag ccctctgaaa gagctgcatc tgctgccagg
    14701 cttttatcac gacacgttgg gtgaagagaa cagggcgcag gcatttgaaa aaatgcaaag
    14761 ctttattagt cgtttatatg ctaacaagtc acaaaaattt gattatcagc atgaagaccg
    14821 cactggacca tcagcggatc gctggcggct cctttcaggt ggacccgtgc cattatcgcc
    14881 ggtcgcttta tgcgaaaggc gatgaaattg ttcggggcgc actctgcggg cctgcatctc
    14941 ggaatgagca ccggctttga ttcaggcagt tccctggatt atgtctatca aaatcaaccg
    15001 caaggcagta acgcattcgg gcgcttaatt gacaaaatct acctgaacag tgttggctgg
    15061 cgcggtattc gccagcgtaa aacccattta caaatactga ttaaacaagc cgttgccgat
    15121 ctccacgcca aaggtttagc cgtccgcgtg gttgacattg ccgcagggca tgggcgctat
    15181 gtactggatg cgctggcaaa cgagcctgcc gtaagcgata ttttgttacg tgattacagt
    15241 gagttaaatg ttgcacaggg gcaagcgatg attgcccaac ggggaatgtc tgggcgggtg
    15301 cgttttgaac agggcgatgc gtttaacccg gaggaactca gcgcgttaac tccgcggcct
    15361 acgctggcga ttgtctctgg cctgtatgag ctttttcccg aaaatgagca ggtaaaaaac
    15421 tcactcgcag gtcttgccaa tgccatcgaa ccgggcggca ttctcatcta caccgggcag
    15481 ccgtggcacc ctcaactgga gatgattgcc ggggtgttaa ccagtcataa agatggtaaa
    15541 ccgtgggtaa tgcgcgtgcg ttcgcaaggg gagatggatt cactcgtgcg tgatgccgga
    15601 tttgataaat gcacacaacg gattgatgag tggggtattt ttacggtttc gatggcggtg
    15661 cgtcgtgata actgaacgtc ggaacgtatt gctacaaggc gctggctggt tattgttgct
    15721 ggccccgttt ttcttcttca cctatggatc tcttaatcag ttcaccgcgg ttcaggacct
    15781 taacagccat gatatcccca gtcaggtatt cggttgggaa acggcgatcc cttttcttcc
    15841 ctggactatt gttccttact ggagtctgga tcttttatat ggattttcgc tgttcgtttg
    15901 tagcacgaca ttcgaacagc gccgacttgt ccaccggctt attctggcaa cggtaatggc
    15961 ctgctgcggt tttttgctct atccgctgaa gtttagtttt atccgtcctg aagtgagtgg
    16021 ggtgacggga tggctatttt cgcaacttga actgtttgat ctgccttata accagtctcc
    16081 ttcgctgcat attattctct gctggctact ttggcgtcac tttcgtcagc atctggctgt
    16141 gaggtggcgt aaagtctgcg gcggatggtt tttactcatc gccatttcga cgctgacgac
    16201 atggcagcat cattttattg atgtcatcac agggctggcg gtaggtatgt tgattgactg
    16261 gatggtgccc gtcgaccgtc gttggaatta tcagaaacct gatcaacgtc gaatcaaaat
    16321 agcactgcca tatgtcgtag gcgcgggctc gtgcattgtg ttgatggagc taatgatgat
    16381 ggttcagtta tggtggtcag tctggttatg ttggccagta ttatcgctac tcattattgg
    16441 ccgggggtac ggtgggcttg gcgcgataac aacagggaaa gatagtcagg ggaaactccc
    16501 gcccgccgtt tactggctga cattgccctg gcgcattggg atgtggctat ctatgcgttg
    16561 gttttgtcgt cgcctggagc cggtgagcaa aatgactgct ggtgtttatt taggggcgtt
    16621 tccacgacat attccggcac agaatgcggt tctggacgtc acctttgaat tccctcgcgg
    16681 acgagcgaca aaagatcgac tctatttctg tgtaccgatg ctggatctgg tggttccgga
    16741 agagggggag ctccgacagg ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag
    16801 cgttctggtc cattgcgcgt tgggattatc gcgcagtgcg ctggtagtgg cggcatggtt
    16861 gttatgttac ggacactgta aaaccgttga tgaagcgatt agttatattc gagccagacg
    16921 ttcgcatatt gtgcttaagg aagatcacaa agcgatgctg aaattatggg aaaacaggta
    16981 agtggattga gatgtggact gaactacagt ccacatcaag atcgtgtccg gttatgcagc
    17041 aacaatgctg tcgatggccg ctttcgcgtc agactgcgct ttcgctgcca tttccggacc
    17101 gtatgcgatc ccttcggcga agacaaattt cacatcggta atgccgataa agccgaggaa
    17161 cgtggacaga tacggcgtca ccaggtccgt tggtccatct ttgtggatcc caccgcggct
    17221 ggtaataacg atggcttttt tacccgttac cagaccttcc ggaccgttct cggtatagcg
    17281 gaaagtaacg cctgcgcgtg caaccaggtc aaaatagttt ttcaactgag ttgagatgtt
    17341 gaagttatac atcggtgccg caataacgat aacgtcgtgg gctttcagct cggcaatcag
    17401 ctcatcggaa agcgccagag cttcctgctg acgcggagtc agcggcgcat cgctcggacg
    17461 cagtgcacca accagttcgc catccagtac cggaatcgga tttgcagcca ggtcgcgaac
    17521 ggtgatttca tcagcggagt gcttttcgcg ccattgttca acaaaataat cggacaactg
    17581 attagactga gagtaccctg ccaggatgct ggatttaaga actaatacct tgctcatggt
    17641 gtttccttat agatgtttga atgggcgatg ccccgttgct tgctgacact ttattcacaa
    17701 tcctgccaca gagatagcgc aataaatcga agcctatgtt cgaatttatt gaacaacgca
    17761 tagaaagccg cgatgtggta ctctatatct atcatttaaa agaaaattaa tcaggcagac
    17821 tactgcccac taacgttatg acagaacaac aaaaattgac ctttacggcc ttgcagcagc
    17881 ggctggattc gctgatgctg cgcgacagac tgcgtttttc tcggcgtctg cacggtgtta
    17941 agaaggttaa aaatcctgat gcacaacagg ccattttcca ggagatggcg aaagagattg
    18001 accaggcggc agggaaagtc ctgctgcgtg aagcggcacg accggaaatt acttatcctg
    18061 acaatttacc ggttagtcag aaaaaacagg acattctcga agcgattcgt gatcaccagg
    18121 tggtgatcgt cgccggggaa acgggttctg gtaaaacgac tcagttaccg aaaatctgta
    18181 tggagctggg gcgcgggatt aaagggctga tcggccatac ccagccgcgt cgcctggcgg
    18241 caagaacggt ggcgaaccgt attgcggaag agctgaaaac ggagccgggc ggttgcatcg
    18301 gttacaaagt gcgtttcagc gatcacgtta gtgataacac gatggtcaag ctgatgaccg
    18361 acggtatcct gctggcggag atccagcaag accgcctgct gatgcagtac gacaccatca
    18421 ttattgacga agcacacgaa cgcagcctga atatcgattt tctgctcggc tatttgaaag
    18481 agttgctgcc gcggcgtcct gacctaaaaa tcattatcac ttccgcgact atcgacccgg
    18541 aacgcttttc gcgccacttt aataatgcgc cgattatcga agtctccggt cggacctatc
    18601 cggtggaagt gcgctatcga ccgattgttg aagaagccga cgacaccgag cgcgatcagt
    18661 tgcaggcgat ttttgacgcc gtagacgaac tgagtcagga aagccctggc gacattctga
    18721 tctttatgag tggtgagcgg gaaattcgcg ataccgccga tgcgctgaac aagctaaatc
    18781 tgcgtcatac cgaaatcttg ccgctttatg cgcggctttc gaacagcgaa cagaaccgcg
    18841 tgttccagtc gcacagcgga cggcgcattg tgctggcgac caacgtcgcg gaaacctcgc
    18901 tgaccgtacc ggggattaaa tacgttatcg accccggtac agcacgtatc agccgctaca
    18961 gctatcgcac caaagtgcag cgtttgccga ttgaacccat ttcgcaggcg tctgccaatc
    19021 agcgtaaagg ccgctgtggg cgtgtgtccg aagggatctg tattcgtctc tattccgaag
    19081 acgatttcct ttcgcgcccg gagtttaccg atccggagat tctgcgtacc aacctggcct
    19141 cggttatttt gcagatgacc gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg
    19201 aagcaccgga taaacgcaat atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga
    19261 tcaccacgga tgaacaggcc agcgcctata aactgacgcc gctcggtcgc cagctctcgc
    19321 agttgcctgt cgatccacgt ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg
    19381 tgcgtgaggc gatgattatc acgtccgcgc tttccattca ggatccgcgc gagcgtccga
    19441 tggataaaca gcaggcatcg gacgaaaaac atcgtcgctt ccacgacaaa gagtctgact
    19501 tcctcgcgtt tgtgaatctg tggaattatc ttggcgagca gcaaaaggcg ctttcttcca
    19561 acgccttccg tcgcctgtgt cgtaccgatt atctcaacta tctgcgcgtg cgcgaatggc
    19621 aggatatcta cacccagttg cgccaggtgg tgaaagaact tggcattccg gttaacagcg
    19681 aaccggcgga gtatcgcgaa attcacattg cgttgctgac gggtttactt tcccatatcg
    19741 gcatgaaaga tgccgataaa caagaatata ccggcgcacg taacgcgcgt ttctccatct
    19801 ttcccggttc tggtttattc aaaaaaccgc cgaaatgggt aatggtggcg gaactggtag
    19861 aaaccagccg cctgtggggg cgcattgctg cgcgtatcga cccggaatgg gtggagcctg
    19921 tcgctcagca tttgattaaa cgcacctaca gcgaacctca ctgggaacgg gcgcagggcg
    19981 cggtgatggc aacggaaaaa gtcactgttt atggtttgcc gattgttgcc gcgcgcaagg
    20041 tcaactacag ccagatcgat ccggcgttat gccgtgaact ctttattcgc catgcgctgg
    20101 tggaaggtga ctggcagacg cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg
    20161 aagtggaaga gctggaacac aaatcacgtc gccgcgatat tctggttgat gacgaaacgt
    20221 tgtttgagtt ctacgaccag cgcatcagcc acgatgtaat ctccgctcgt cacttcgaca
    20281 gctggtggaa aaaagtcagc cgcgaaaccc ctgatttgct caactttgaa aaaagcatgt
    20341 tgatcaaaga gggcgcagaa aaaatcagca agctggatta cccgaacttc tggcatcagg
    20401 gcaatctcaa gctgcgtttg agctatcagt ttgagcccgg cgcggatgct gacggtgtga
    20461 ccgtacatat tccgctgccg ttacttaacc aggttgagga aagcgggttt gaatggcaga
    20521 tccccggtct gcgccgcgaa ctggtgattg ctctgattaa atcgttgccg aaaccggtac
    20581 gccgtaattt tgtacccgcg ccaaactatg ccgaagcgtt tttaggccgc gtcaaaccgc
    20641 tggagttacc gttgctcgac agccttgagc gcgagttacg gcggatgacc ggcgttaccg
    20701 ttgaccgcga agactggcac tgggatcagg tgcctgatca cctgaaaatt accttccgcg
    20761 tggtggatga caaaaacaag aagctaaaag aagggcgctc gctacaagat ctgaaagatg
    20821 cgctgaaagg caaagtgcag gaaacgctat ctgcggtggc ggatgacggt atcgagcaga
    20881 gcggcttaca tatctggagt tttggtcagc tgccggaaag ctacgaacag aagcgtggca
    20941 actacaaagt gaaggcgtgg ccggcgctgg tggatgagcg cgacagtgtg gcgatcaaac
    21001 tgtttgataa cccgctggag caaaagcagg caatgtggaa cggtcttcgc cgtctactgc
    21061 tgctgaatat tccatcgcca atcaaatatt tacatgaaaa gttaccgaac aaagccaagc
    21121 tgggactgta ctttaacccg tatggcaaag tgctggagct gatcgacgac tgtatctcct
    21181 gcggtgtgga tcaattgatc gacgccaatg gtggcccggt ctggacggaa gaaggctttg
    21241 ctgcgctgca tgaaaaagtg cgcgccgaac tgaatgacac ggtggtggat attgcgaagc
    21301 aggtcgagca aattcttacg gcagtgttca atatcaacaa acgtctgaaa gggcgggtgg
    21361 atatgaccat ggcactgggg ctttctgaca ttaaagcgca gatgggcggg ttggtatatc
    21421 gcggttttgt cactggtaac ggcttcaaac ggctgggcga cacgctgcga tatttgcagg
    21481 cgattgaaaa acggctggaa aaactggcgg ttgatccaca tcgcgaccgt gctcagatgc
    21541 tgaaagtcga aaacgtccag caggcgtggc agcaatggat caacaaactg ccgcctgcac
    21601 gtcgtgagga tgaagacgtg aaagagatcc gttggatgat agaagagttg cgcgttagtt
    21661 acttcgctca acaacttggt acgccttatc cgatttcaga taagcgtatt ttgcaggcga
    21721 tggagcagat tagcggttaa ccctgctatt tgcctgataa agaaaaaacc cggtaagcat
    21781 ttagcgccgg gttttttatt aattctaaaa cggtaagggt aaaaattcag gaattcagaa
    21841 aaatacaatt ctctgctgca agatgaataa tgtttatcta cagcatttcc ttaaaagata
    21901 tgtcaggctt gcggagtggc ggttaaggac atacgacttc ctcctttcag agtgctccgc
    21961 ttctcactat tatctcacgc agtattctta agggaacgat aaggaggaac catgaacatt
    22021 accccgtttc cgacgctttc gccggcaact atagatgcca taaatgttat cggacagtgg
    22081 ctggcgcagg atgatttctc cggtgaggtg ccgtatcagg ccgattgcgt gatccttgca
    22141 ggcaatgcgg ttatgccgac tatcgacgcg gcatgtaaga ttgctcgcga tcagcaaatt
    22201 cctttactga ttagtggtgg tatcggtcac tcgacaactt ttttgtatag cgccatcgca
    22261 cagcatccgc actacaacac tatccgcacc actggcagag cagaagcgac catcctggcg
    22321 gatatcgctc atcagttctg gcacattccg catgaaaaaa tctggattga agaccagtca
    22381 acaaactgcg gtgaaaacgc acgctttagc atcgcgctat tgaaacaggc cgtagaacga
    22441 gttcatacgg ctatcgttgt tcaggacccc accatgcagc ggcgcacgat ggcgacgttc
    22501 cgccgtatga ctggggacaa tcccgatgca ccacgctggt taagttatcc cggattcgtt
    22561 cctcagttag gaaataatgc agacagtgta atctttatta atcagttaca aggattatgg
    22621 ccggttgagc gttatctctc actactcact ggcgagctgc cgcgtttacg cgatgatagc
    22681 gatggctacg gtccccgcgg gcgagatttt atcgttcacg ttgattttcc ggcagaagtc
    22741 atccatgcat ggcaaacgct gaaacatgat gcggtgctca tcgaggcgat ggaaagtcgc
    22801 tcgttacgtt aaaaattgcc cgtttgtgaa ccacttgttt gcaaacgggc atgactcctg
    22861 acttttattt ctgcctttta ttccttttac acttgttttt atgaagccct tcacagaatt
    22921 gtcctttcac gatgccgtct ctctgatgat tgatgttaat taacaattca ttcaccgaaa
    22981 acaaacatat aaatcacagg agtcgcccat gtcagtaccc gttcaacatc ctatgtatat
    23041 cgatggacag tttgttacct ggcgtggaga cgcatggatt gatgtggtaa accctgctac
    23101 agaggctgtc atttcccgca tacccgatgg tcaggccgag gatgcccgta aggcaatcga
    23161 tgcagcagaa cgtgcacaac cagaatggga agcgttgcct gctattgaac gcgccagttg
    23221 gttgcgcaaa atctccgccg ggatccgcga acgcgccagt gaaatcagtg cgctgattgt
    23281 tgaagaaggg ggcaagatcc agcagctggc tgaagtcgaa gtggctttta ctgccgacta
    23341 tatcgattac atggcggagt gggcacggcg ttacgagggc gagattattc aaagcgatcg
    23401 tccaggagaa aatattcttt tgtttaaacg tgcgcttggt gtgactaccg gcattctgcc
    23461 gtggaacttc ccgttcttcc tcattgcccg caaaatggct cccgctcttt tgaccggtaa
    23521 taccatcgtc attaaaccta gtgaatttac gccaaacaat gcgattgcat tcgccaaaat
    23581 cgtcgatgaa ataggccttc cgcgcggcgt gtttaacctt gtactggggc gtggtgaaac
    23641 cgttgggcaa gaactggcgg gtaacccaaa ggtcgcaatg gtcagtatga caggcagcgt
    23701 ctctgcaggt gagaagatca tggcgactgc ggcgaaaaac atcaccaaag tgtgtctgga
    23761 attggggggt aaagcaccag ctatcgtaat ggacgatgcc gatcttgaac tggcagtcaa
    23821 agccatcgtt gattcacgcg tcattaatag tgggcaagtg tgtaactgtg cagaacgtgt
    23881 ttatgtacag aaaggcattt atgatcagtt cgtcaatcgg ctgggtgaag cgatgcaggc
    23941 ggttcaattt ggtaaccccg ctgaacgcaa cgacattgcg atggggccgt tgattaacgc
    24001 cgcggcgctg gaaagggttg agcaaaaagt ggcgcgcgca gtagaagaag gggcgagagt
    24061 ggcgttgggt ggcaaagcgg tagaggggaa aggatattat tatccgccga cattgctgct
    24121 ggatgttcgc caggaaatgt cgattatgca tgaggaaacc tttggcccgg ttctgccggt
    24181 ggtcgcattt gacacgctgg aagatgctat ctcaatggct aatgacagtg attacggcct
    24241 gacctcatca atctataccc aaaatctgaa cgtcgcgatg aaagccatta aagggctgaa
    24301 gtttggtgaa acttacatca accgtgaaaa cttcgaagct atgcaaggct tccacgccgg
    24361 atggcgtaaa tccggtattg gcggcgcaga tggtaaacat ggcctgcatg aatatctgca
    24421 gacccaggtg gtttatttac agtcgtaatg agtaaaagag gcggaggttt tttcctccgc
    24481 ctgtgcgcgt cagagtttag cgaatttttc gagggtgcga ataagctgcg tgacgaagcc
    24541 atattcgtta tcgtaccagg cgaccgtttt caccagttgt aaatcgccca cggcggtaat
    24601 ttccgtttgc gtggcatcaa acaccgaacc gaaatggctg ccaatgatat cggaagagac
    24661 tatttcttca tcggtataac caaatgactc gttattggtg gttgcttgtt taagtgcgtt
    24721 attcacctct tcggcagtca ctttttttcc gagaatggac accagctcag tgaccgaacc
    24781 tgttttcacc ggcacgcgtt gcgcatgacc tttcagtttg ccgctcagtt ccgggatcac
    24841 cagaccaatg gcttttgccg cccccgtagt gtggggaatg atattttctg ccgctgcgcg
    24901 tgaagcacgt aaatctttac cacgtgggcc atccaccagt gactgggtgc cagtataggc
    24961 atgaatggtc gtcatcgtgc cgacttctat cccgaaactg tcatgcaagg ctttggccat
    25021 cggcgcaaga cagttagttg tgcatgacgc cacggaaaca atggtgtcgt tgccatccag
    25081 agtgtcgtca ttgacgttat aaacgatagt tttcatttca ccggcagggg cggaaatcaa
    25141 caccttcttc gcaccagcat caagatgcgc ctgcgatttc tcggcggagg tataaaagcc
    25201 agtacattcg acaatgattt ctgcaccttt cgctttccac ggaatatttt tagcctcttt
    25261 ttcggcgtaa accgcgatac ttttcccatc aacgataagt gaatcttccg taaaatcaac
    25321 gctccagggg aatggtccgt agtttgaatc atgtttcagc aggtaggcga gaatttttgg
    25381 ggaagtgaga tcattaatag cgacaacgtc tatgttgctt ttgacttcaa gtaatcgacg
    25441 caacaccagt cgaccgatac gaccaaaacc gttaatacca actttactca tggttttctc
    25501 ctgtcaggaa cattcggatg aaaattgatc ctttccaggc ttagaccagg attgtgggat
    25561 gggcaatctc cattcttaca gtgaaacgta acgtactgaa aacgggtgaa caatatttaa
    25621 tgaaattttg agaaaagccc gttatgttaa cggaaaatta tgttaaagca ggaaatgtta
    25681 tggaaaataa atactcaagg ttacaaatca gcattcactg gctggtcttt ttgctggtta
    25741 tcgcagcgta ttgcgcaatg gagtttcgtg gtttcttccc acgtagcgat cggccactca
    25801 tcaacatggt tcatgtttcc tgtggtatct caattctcgt gctgatggtg gttcgtcttt
    25861 tgttaagact gaaataccca accccgccga ttatacctaa gccaaaaccg atgatgacgg
    25921 gactggcgca tttgggacat ttggtgattt atctcctgtt tattgcgctg ccagtgattg
    25981 gtttggtgat gatgtataac cggggcaacc cgtggtttgc gtttggtttg acgatgcctt
    26041 acgcttcaga ggccaatttc gaacgggttg atagcttaaa gtcgtggcat gagacgctgg
    26101 cgaatctggg atattttgtg atcgggttgc acgctgcggc tgcactggca caccactatt
    26161 tctggaagga caacacactt ctacgcatga tgccgcgtaa acgttcctga aggatatttc
    26221 aagaaaacgc ctgtactaaa accgacccgt ggtacaggcg aagaatacgg gtctacatcg
    26281 gaagcgccta ttatatttat ttgtatgaca aataaaacca taatccttgc ccatacgtcc
    26341 atctggctta tttttaatca attcacccga tctttgatct catcaacggt atcaaaataa
    26401 accagcgtat aacgttcaaa tcgaacataa gattcgataa accatggagg ttatatgaaa
    26461 aaactggcac ttattttgtt tatgggaacg cttgtttcct tttatgccga tgccgggcgc
    26521 aaaccctgtt ctggttcgaa aggggggatc tcacactgta cggcaggcgg caaatttgtc
    26581 tgtaatgatg gttctattag tgcctcgaaa aaaacatgca ctaactgaag tgtaaaaggg
    26641 gtgccatgag aaaatgaatc tgctagtcag atgcgcgggg aaaatccccg cgcttgccct
    26701 tacctggacg tgcaggccat gagcgcagca acctccttat caccgtcccg gaaccgcagt
    26761 tcgtagagcg tttgtcgggt caggagtgtg aatgtcagaa tcgtaatgca gataatgagc
    26821 agacacacca ccagagggtt gtgcttcata gcctttctcc ttgccggatg gcgggtaaga
    26881 ggctaagatc tgaattgcta ggttcattcg ttggcctcgg ttgatagaaa tatcggtcgg
    26941 ggccttcgtc tttctgattc ccggttagcc tgaaaacaga aagtctcagg cacccgcagg
    27001 catcctatga ggtttcctta gggacgaaaa taatcacttc acgaaattgc gtgctgtttt
    27061 ccagaatttt tcgtcattcg ggttagccag tttagccatt cgttactctc ttcattccaa
    27121 tagcattaat tttctctgca ataattgttg taaaaatgtg acgcaaagag gtttttggtc
    27181 ataagtaatt accgtcaagt gccgatgact ttctatcagg agtaaacctg gacgagagac
    27241 aacggtaatg aatacaactc cctcacatcg attaggtttt ttgcatcaca tcaggctggt
    27301 tccgttattt gcctgcattc ttggcggtat cttagttcta ttcgcattaa gttctgctct
    27361 ggctggctat ttcctctggc aggccgatcg cgatcagcgt gatgttactg cggagattga
    27421 gattcggacc gggttagcga acagttcaga ttttttgcgt tcagcccgga tcaatatgat
    27481 tcaggccggg gctgcgagtc gtattgcgga aatggaagca atgaagcgaa atattgcgca
    27541 agccgaatcg gagattaaac agtcgcagca aggttatcgt gcttatcaga atcgaccggt
    27601 gaaaacacct gctgatgaag ccctcgacac tgaattaaat caacgctttc aggcttatat
    27661 cacgggtatg caacctatgt tgaaatatgc caaaaatggc atgtttgaag cgattatcaa
    27721 tcatgaaagt gagcagatcc gaccgctgga taatgcttat accgatattt tgaacaaagc
    27781 cgttaagata cgtagcacca gagccaacca actggcggaa ctggcccatc agcgcacccg
    27841 cctgggtggg atgttcatga ttggcgcgtt tgtgcttgcc ctggtcatga cgctgataac
    27901 atttatggtg ctacgtcgga tcgtcattcg tccactgcaa catgccgcac aacggattga
    27961 aaaaatcgcc agtggcgatc tgacgatgaa tgatgaaccg gcgggtcgta atgaaatcgg
    28021 tcgcttaagt cgtcatttac agcaaatgca gcattcactg gggatgacag tagggactgt
    28081 tcgacagggt gcggaagaga tttatcgtgg caccagcgaa atttcagctg gcaatgcgga
    28141 cctgtcatct cgcaccgaag aacaagcggc ggctatcgaa caaactgccg ctagcatgga
    28201 gcaactcact gcgacggtga aacagaatgc ggataacgcg catcatgcca gcaaactggc
    28261 gcaagaggct tctattaaag ccagcgatgg cgggcagacg gtttccggtg tagtaaaaac
    28321 gatgggcgct atctctacaa gttcgaagaa aatttccgag atcaccgccg tcatcaacag
    28381 tattgctttc cagacgaata ttctggcact gaatgctgcc gttgaagccg cgcgagcggg
    28441 tgagcaaggc cgtggatttg ccgttgttgc cagcgaagta cggacactcg caagccgcag
    28501 cgcccaagcg gcgaaagaga ttgaaggctt gatcagtgaa tcagtcaggt taattgacct
    28561 ggggtcggat gaggtggcaa cggcagggaa aaccatgagc actattgttg atgccgtcgc
    28621 gagtgtcaca catatcatgc aggaaatcgc cgccgcctcg gatgaacaaa gtagaggcat
    28681 aacgcaggtt agccaggcga tttctgaaat ggataaggtg acgcaacaga atgcttctct
    28741 ggtagaagag gcctcagcgg cggcggtgtc ccttgaagaa caggcggcac gattaactga
    28801 ggcggtggat gtattccgtc tgcacaaaca ttctgtgtcg gcagaacctc gcggagcggg
    28861 tgaaccagtt agtttcgcta cggtgtgaaa atgttcaagg agggatcgac agatcccttc
    28921 acctttcaga acggcattga ttttcgaata gcgttaatca tcaactggca accagaagag
    28981 aacgtcgcat ctacgcgggt cagtattcca atcggttcgc ctgcaccatg tcccggaaca
    29041 ggcagggcca ccagcgtggc atgacgcagg tcgtctttta cagcgccaga agggacaaac
    29101 cacacgtaat cgtattcaac cgtaagttga cgagatagcg aagcagacag cgtttcgata
    29161 caacccgaag gaattttaca gccctggctc tgcactaatg catctgaatg ctggcgtggc
    29221 gcagtgcctt ctggtgatac aacgaccggc cattccagca cccggcttag cgttacgttc
    29281 tcctgaagta gcgggtgatt agggcggaca accagcttca acgattcaag aaacagcagt
    29341 tcgtaattaa gcccggtcat cagttcagga tctgacatcc gaccaatgcc gatatcgatt
    29401 tccccggttt ttaaacccgc cagaatcata gggttactca ttgtcgcaac ttgcaaggtc
    29461 gtctcttttt gttgctgatg aaactgacct ataaccgaag gtaatatccc cagtgccgca
    29521 gtaggtagtg caccaaccct gacgacatca ttattaagac cttctttacg atgaagcgac
    29581 tgtccggcag tgttgatggc gtcaagaact ctgactgcat gcgttaaaaa ttgttcgccg
    29641 ggtaaggtaa gttgcgaccc ctgacgacca cgctcaaaca agcgagcgcc agtcagctgc
    29701 tccagttcat tcaatgtctt agagagcgca ggttgactca aattaagggt ttcagccgcg
    29761 cgccccaaag ttccttgttg tgcgacagct acgaatgtat gaaggtggcg caaacggatg
    29821 cgctgactaa acagactatt tttttccata agcgatgtta aaaacgaagc ggtgtcgctg
    29881 acaagtgaag ttgtttgatt atgataactt gattgcaaaa tattattaac aattaaagca
    29941 attgtgttac agcaaaatgg ataatattga tgttttcgcg gcgagatcac agtttgtaaa
    30001 ttcttcccgc cagagtgaat gcggttacct acactccaga ttactgacca ctggaggcag
    30061 acactatggc gaacagcatc acggcggatg agattcggga acagttttcg caggcaatgt
    30121 cagccatgta ccagcaagaa gttccgcaat atggcacgct gctggaactg gtagctgatg
    30181 tgaatctggc tgtgctggaa aacaatcctc aactgcacga aaaaatggta aatgcagacg
    30241 agctggcgcg actgaatgtt gaacgtcatg gggcgattcg cgttgggact gcacaagagc
    30301 ttgctactct tcggcggatg tttgccatta tggggatgta cccggtgagc tattacgatc
    30361 tctcgcaggc aggggtgccg gtacattcga cagcatttcg gcccattgat gatgcttctc
    30421 tggcgcgtaa tcccttccgc gtttttacct ccttactccg ccttgagctt atcgagaacg
    30481 aaattttgcg ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc accccacgtt
    30541 gtcgacaact gttagaggaa tatgagcagc agggcggttt taacgaaaca caggcacagg
    30601 agtttgtgca ggaagccctg gaaacgtttc gctggcacca gtcagcaacg gtagatgaag
    30661 aaacctatcg cgccttgcac aacgaacatc ggttgattgc tgatgtggtc tgttttcctg
    30721 gatgccatat caaccacctg acgccacgta cgctggatat tgaccgggtg cagtcgatga
    30781 tgcctgaatg cggaattgaa ccaaaaattc tgatcgaagg gccgccgcgc cgcgaggtac
    30841 cgattttact acgccagacc agctttaaag cactggaaga gacggtgttg tttgcggggc
    30901 agaaacaggg cacgcatact gcgcgctttg gtgaaataga gcagcgtggt gtggcattaa
    30961 cgccgaaagg gcgacaactg tatgatgatc ttctgcgtaa cgctggaacc gggcaggata
    31021 atctcactca ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac agtgagtttt
    31081 taatgcgtca gcaagggttg gcatggttcc ggtaccgtct gacgccttcg ggtgaggcgc
    31141 atcgtcaggc gattcatcct ggagacgatc cacagccctt aattgaacgt ggttgggtag
    31201 tggcgcaacc catcacctat gaagatttct tgcccgttag cgcggcgggg atcttccagt
    31261 caaatctggg taatgaaacg cagacacgca gtcacggtaa tgccagtcgc gaagcatttg
    31321 agcaggcgtt gggttgtccg gttttggatg agttccagct ttaccaggaa gcggaagaac
    31381 gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc
    31441 ctgattttac aaacgaatca ggctcatccc atcgacataa aaaaaatgcc gatttatgca
    31501 tattctctca gttcaacaat tggattatta ataaatattg tctagagtga gcggtcataa
    31561 ataagcactt tcttgccgct gaaaacgacc agcgcgggac cattcacaac accagaagga
    31621 ctcactttca ggtatggatc gtagacgatt tattaaaggt tcaatggcta tggccgccgt
    31681 gtgcggtacc agcggcattg cctctctttt ttctcaggcg gcattcgcgg cagattctga
    31741 tattgccgac gggcaaaccc agcgttttga cttctccatt ctacagtcaa tggcgcacga
    31801 cttagcgcaa acagcgtggc gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat
    31861 gacgccgcag gcttataaca gtattcaata cgacgccgaa aaatcgctct ggcataacgt
    31921 tgagaaccgt caactggacg ctcagttctt ccatatggga atgggattcc gtcgccgcgt
    31981 tcgtatgttt tctgtagatc cagcaacaca tctggcgcgt gaaattcact ttcgcccgga
    32041 gttgttcaaa tacaacgatg caggtgttga tacaaaacaa ttagaagggc aaagcgatct
    32101 cggctttgcc ggttttcgcg tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc
    32161 atttctcggc gcgagttatt tccgcgccgt tgatgataca tatcaatacg gtttgtcggc
    32221 ccgcggcctg gcgatcgaca cttacaccga cagtaaagaa gagttccccg actttaccgc
    32281 cttctggttt gatacggtaa aaccgggggc aactaccttt accgtttatg cgttgctcga
    32341 tagcgccagc attactggtg cctataagtt cactatccat tgtgagaaaa gtcaggtgat
    32401 tatggatgtg gaaaatcacc tgtatgcgcg caaagacatt aaacagctgg gcattgcgcc
    32461 gatgaccagt atgttcagct gcggtactaa tgaacgtcgg atgtgcgata caattcatcc
    32521 gcaaattcat gactctgatc gtctgtccat gtggcggggc aacggcgagt ggatttgccg
    32581 tccgctgaat aatccgcaaa aattgcaatt caatgcttac accgacaaca acccgaaagg
    32641 gtttggttta ttgcaactgg atcgtgactt ctcccattat caggacatta tgggctggta
    32701 taacaaacgc ccaagtctgt gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg
    32761 cctgatggaa atcccaacaa cgggcgaaac gctggataac attgtctgct tctggcagcc
    32821 agaaaaagct gtaaaagcag gtgatgagtt tgcattccag tatcgtctgt actggagtgc
    32881 gcaaccgcct gttcattgcc cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg
    32941 tttcccggaa ggttgggcgc caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc
    33001 cgtcgatttc gttggtggtg atctgaaagc tgccgcgcca aaaggcattg agccggtgat
    33061 tacgctttcc agtggggaag cgaagcaaat cgaaattctc tatattgaac ccatcgatgg
    33121 ttatcgtatt cagtttgact ggtatccgac ttcggactcc actgatccgg tcgatatgcg
    33181 gatgtatcta cgttgtcagg gggacgctat cagtgaaaca tggctgtatc agtatttccc
    33241 gccagcgccg gataaacgtc agtatgttga cgaccgcgtg atgagttaat cgttttttct
    33301 tcggcacctt cttcgggagg tgccgtctgg ttaaacacga tcccgctcgc atttttccct
    33361 aagttaaatg agtaatctga tggtgtgtat ttcagataca ccttgtcagc cactaacagg
    33421 gagtgcgtat gtttccagaa taccgagatt taatatcccg tctgaaaaac gaaaatcctc
    33481 gctttatgtc cttgttcgat aaacacaata aacttgatca tgaaattgcc agaaaggaag
    33541 gttccgacgg tcgagggtac aatgcggaag tggtccgcat gaaaaaacaa aagctacagt
    33601 taaaagatga gatgctcaaa atcctgcagc aggagagcgt caaagaggtg taaactttcc
    33661 taagccgcca gccaggcggc ttttttaaca acagcatgga ttgactggag ataagatgac
    33721 tgaaacgata aaagtaagcg aatcacttga attacatgct gttgcagaaa atcacgtcac
    33781 tcctctttat cagttaatct gtaaaaataa aacctggtta cagcagtcgc taaactggcc
    33841 gcagttcgtt cagagtgaag aggacacgcg aaaaacggtg cagggtaatg tgatgttgca
    33901 tcaacgcggc tatgccaaaa tgttcatgat tttcaaagaa gatgaactta tcggcgttat
    33961 ctcgtttaat cgtattgaac cactgaataa aaccgctgaa ataggctact ggctggacga
    34021 atctcatcag gggcagggga tcatttctca ggcgctgcag gcattgattc atcattacgc
    34081 ccagtctggt gaacttagac gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa
    34141 ccaggtcgct ttgcgcaatg gttttatcct tgaaggttgc ctgaaacagg ctgagttcct
    34201 gaatgatgcc tatgatgatg tgaacttata cgcgcgtatt atcgattcat aataaccctg
    34261 ccagcggcgt tcgcgtaatg cgctcctcgc cgttaatgac ttttggacca cggacatgaa
    34321 ccgtgtcgcc atcaaaggct tcaacgacgg cgtggtcggt taactccaca tggttttcaa
    34381 ttatcaccgc acctgtaata cggctctcac cttgtattac aacgtgctcg tcgagcagaa
    34441 tcggtccacc acgtaccacg gcatttccgc caatcaagac atgatgtttt aacacacaat
    34501 taccttccac aatggcatat tccgccacct gcgaactgta atgaatcgtt ggaatggcat
    34561 cttcttctat gccagctttc acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc
    34621 agacgttgtt ctcttcatta ccttcaatac tggcaaaatc aaaaacttcg gcacgatgtt
    34681 caataaaagc ataccggatg acggcatcgc cataaatttg tgcctggtgg acaatacgcg
    34741 aggcgctaac ccttgcgcga tcataaattt gtagcaggag ttgatggtca ggcgttaagc
    34801 cttgtgctgc gacgatcata gaatgttgat taattaaggc gtgaccaaat attctacatt
    34861 gcccgcaaac cagtgagtca tgaatggtga cgctgtcact tatatatgca ccctggctga
    34921 tttcactatt atcgatccag acattatccg ttgcgtaaac ctctccccac agcacgctgg
    34981 tgccggtgat gcgcgtatta ccggaaatca ccgctccgcc gaacgcaata gcgttctggt
    35041 cgtaaatcca gcaatttcct tcctgtgcta acaccgtttc gcgatcgatc cagccgccag
    35101 cggttcctgc tatcacatcg ttaaaatcgc taatggcgat aatctgccgt aataacacat
    35161 tttttttagt gccatcttcc tgataactaa aggctcgctg ttcttcactt agacgatatt
    35221 tgcgcatagc gttttcccac aggtgactta ctataaccgt agcaaattct gcggctctgg
    35281 ctatgctcga gaaaattcca taaaatgcat ttcaaatata ctttataaat taaacaaaat
    35341 gagtaagaag atgcagagcg ataaagtgct caatttgccg gcaggctact ttggtattgt
    35401 gttggggacg atagggatgg gatttgcctg gcgctatgcc agccaggttt ggcaggtcag
    35461 ccactggtta ggggatgggc tggtgattct ggcgatgatc atctggggat tgttaactag
    35521 cgcatttatt acccgactca tacgctttcc gcatagcgtg ctggcagaag tgcgccatcc
    35581 agtgatgagc agttttgtga gtttgtttcc ggcaacgacg atgctggtgg cgattggttt
    35641 tgttccgtgg tttcgcccac tggcggtgtg cctgttcagt tttggtgtcg tggttcagtt
    35701 ggcttatgcc gcctggcaaa ctgcgggatt atggcgcgga tctcaccctg aagaagctac
    35761 cacgcctgga ctgtatctgc cgacagttgc caacaacttt atcagcgcaa tggcctgtgg
    35821 tgcgttgggc tacaccgacg ccggtctggt gtttttaggc gcaggcgttt tctcatggct
    35881 aagccttgaa cctgtgatct tgcagcgtct gcgcagttcg ggagaattac ccacggcact
    35941 gcggacatca ctcggcattc agttcgctcc tgcgctggtg gcctgtagtg cttggttgag
    36001 cgtcaacggc ggcgagggtg atacgctggc gaaaatgctt tttggttatg gactgctgca
    36061 actgctgttt atgctacgtc tgatgccatg gtatctctcc cagccattta atgcttcatt
    36121 ctggagtttc tcgttcggcg tatctgcact ggcaaccacc ggtttgcatc tggggagtgg
    36181 cagcgaaaat ggatttttcc atacgctggc ggtaccgctg tttatcttta ccaattttat
    36241 tattgcaata ctgctcatcc gtactttcgc gcttctgatg cagggaaaat tgttagtcag
    36301 aaccgagcgc gccgttttaa tgaaagcaga ggacaaagaa tgatcattcg tgacgaaaac
    36361 tattttactg ataaatatga attaacccgc acacactctg aagtactgga agcggtgaaa
    36421 gtggttaaac cgggtaaaac gctggatctg ggctgtggca atggtcgtaa cagtctttac
    36481 ctggcagcca atggttatga tgttgacgca tgggataaaa atgccatgag tatcgccaac
    36541 gtcgagcgca ttaaatccat tgaaaatctg gataatttac acacccgggt cgttgatctg
    36601 aataacctca catttgatgg acagtacgat tttattcttt cgactgtggt gctgatgttc
    36661 cttgaggcta aaaccatccc cgggttgatt gccaatatgc aacgttgcac taaacctggt
    36721 ggttacaacc tgattgtggc ggcgatggat accgctgatt atccatgtac cgtcggcttc
    36781 ccgtttgcct tcaaagaggg agaattacgt cgatattacg aaggctggga gagggtgaaa
    36841 tacaatgaag acgtcggcga gctgcaccgc accgacgcca acggtaatcg tattaaactg
    36901 cgtttcgcca cgatgctggc acgtaaaaaa tgacccgcta agaataaggc gcgtgaaaat
    36961 tccccacgct gagatgattt actgttcttc ttttcggtaa gcatattttt tatcgaaggg
    37021 atgtgaaatt aatcacagta gtcgaagttt tttagcagct taacttactg aaatttaact
    37081 actgatgatt gacttagccc ctttttcggc attgactatg tcgtctgaaa aggggctgaa
    37141 aaatttattt tcaccaacac tttttttgcc acaacacgaa gcggcgcttt ttgctataac
    37201 ttaaaaagta atataatcat ctcaggaaac tattcatgcg taccacatca tttgcgaaag
    37261 ttgcagcttt atgcggcttg ttggctctgt ctggttgtgc ctctaaaatc acccagccag
    37321 ataaatattc tggtttttta aacaattact ctgatttaaa agaaacaacc tcagcaacag
    37381 gtaaacctgt tttacgttgg gtagacccga gttttgatca aagcaaatat gacagcatcg
    37441 tctggaaccc aatcacttat tatccggtgc caaaaccttc tacccaggta gggcagaaag
    37501 ttctggataa aattttgaac tataccaaca ccgaaatgaa agaagcgata gcgcagcgta
    37561 aaccagtggt taccaccgct gggccgcgta gtctgatttt ccgtggggcc attaccggtg
    37621 tagataccag caaagaaggg ctgcaattct atgaagtggt tcctgttgca ttagtggttg
    37681 cggggacgca aatggctaca ggccaccgta ccatggatac tcgcctctat tttgaaggtg
    37741 agctgattga tgcagcgact aataaaccgg ttatcaaagt cgttcgtcag ggcgaaggta
    37801 aagacctgaa taacgaaagt acgccaatgg ctttcgaaaa tattaaacaa gttattgatg
    37861 acatggcgac cgatgccacc atgtttgacg ttaacaaaaa gtagttccag acgcgccatc
    37921 gttagatggc gctttttatc cggtgcgccg taaaacccc
//
LOCUS       DAEVZA010000032        37950 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEVZA010000032 DAEVZA010000000
VERSION     DAEVZA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.032"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..244
                     /locus_tag="LAF92_001698"
     CDS             <1..244
                     /locus_tag="LAF92_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBI9790718.1"
                     /translation="TTKSNDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCE
                     YDSHANPVDCQLIIVDEGVKPAVERVYTIKNTIDYY"
     gene            complement(247..807)
                     /gene="speG"
                     /locus_tag="LAF92_001699"
     CDS             complement(247..807)
                     /gene="speG"
                     /locus_tag="LAF92_001699"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HBI9790719.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(842..1183)
                     /locus_tag="LAF92_001700"
     CDS             complement(842..1183)
                     /locus_tag="LAF92_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBI9790720.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1318..1644
                     /locus_tag="LAF92_001701"
     CDS             1318..1644
                     /locus_tag="LAF92_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBI9790721.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1850..3064
                     /gene="rspA"
                     /locus_tag="LAF92_001702"
     CDS             1850..3064
                     /gene="rspA"
                     /locus_tag="LAF92_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBI9790722.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            3076..4095
                     /locus_tag="LAF92_001703"
     CDS             3076..4095
                     /locus_tag="LAF92_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HBI9790723.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADRAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            4153..4263
                     /locus_tag="LAF92_001704"
     CDS             4153..4263
                     /locus_tag="LAF92_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBI9790724.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHAR"
     gene            complement(4283..5563)
                     /locus_tag="LAF92_001705"
     CDS             complement(4283..5563)
                     /locus_tag="LAF92_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI9790725.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(5598..5849)
                     /locus_tag="LAF92_001706"
     CDS             complement(5598..5849)
                     /locus_tag="LAF92_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBI9790726.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(5922..8393)
                     /locus_tag="LAF92_001707"
     CDS             complement(5922..8393)
                     /locus_tag="LAF92_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBI9790727.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFEGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPSAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAFHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDLEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGSGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            complement(8487..8678)
                     /gene="ydfD"
                     /locus_tag="LAF92_001708"
     CDS             complement(8487..8678)
                     /gene="ydfD"
                     /locus_tag="LAF92_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HBI9790728.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(8675..8863)
                     /gene="dicB"
                     /locus_tag="LAF92_001709"
                     /gene_synonym="ftsT"
     CDS             complement(8675..8863)
                     /gene="dicB"
                     /locus_tag="LAF92_001709"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HBI9790729.1"
                     /translation="MKMLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(9350..9925)
                     /locus_tag="LAF92_001710"
     CDS             complement(9350..9925)
                     /locus_tag="LAF92_001710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790730.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALRIRMCSHNDA
                     EHKYEKQNQTAENFVLEPGETLFKIPVTCPICGFTSEELDDSCNNQETTKYVEDDTEC
                     ARRTIISTSPNSRTNKSHFERVINPLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQR
                     SANNCKRFIPVINFHVLKRIYWWLRHCLMNP"
     gene            complement(9927..10082)
                     /locus_tag="LAF92_001711"
     CDS             complement(9927..10082)
                     /locus_tag="LAF92_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBI9790731.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(10275..10682)
                     /locus_tag="LAF92_001712"
     CDS             complement(10275..10682)
                     /locus_tag="LAF92_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI9790732.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            10760..10987
                     /locus_tag="LAF92_001713"
     CDS             10760..10987
                     /locus_tag="LAF92_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI9790733.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            10971..11492
                     /locus_tag="LAF92_001714"
     CDS             10971..11492
                     /locus_tag="LAF92_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790734.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTYEGGAVDQRALSVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYESSFC"
     gene            11473..12438
                     /locus_tag="LAF92_001715"
     CDS             11473..12438
                     /locus_tag="LAF92_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790735.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLNESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDPTENTT
                     EITTENKNTFSPEASQPDPQTAEQDFLIRHPDAVVFSTKKRQWGSHEDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            12479..12904
                     /locus_tag="LAF92_001716"
     CDS             12479..12904
                     /locus_tag="LAF92_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBI9790736.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLSDG
                     EIRRYDSRLNIICSECRRSEVMQRILAFYQGNVRYFRRY"
     gene            complement(13277..13984)
                     /locus_tag="LAF92_001717"
     CDS             complement(13277..13984)
                     /locus_tag="LAF92_001717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790737.1"
                     /translation="MGTEIENKQLEINTPSTVSSYTAEKNSNSSVTEVASSFAAILII
                     VVIVVLILCLLQKMHVAITRFFEFIISVPWYKRTVLIVIGAISTYLTTKVPTSYSFLP
                     IIIGYITTFAFNEFASKKDIEKAKAPLEEDIIGKEKTIVLFRRIIEEKKNIFQEIIIN
                     RINACVRITGKNDSDTLRKLKIKICELQDIIFAEKTFTETPDEAIRRYENSVSGDVDM
                     AAQTMFGDSRSEQATAA"
     gene            complement(13994..14260)
                     /locus_tag="LAF92_001718"
     CDS             complement(13994..14260)
                     /locus_tag="LAF92_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790738.1"
                     /translation="MNSFNTNAMKIGKDKQSLLSIANNFNRKSIGIKSTIIEEEQTPL
                     IVFTVEEIAVDEKMLNLSGDSLRMLAAILAGAPTLFEKLGMAKD"
     gene            complement(14637..14831)
                     /locus_tag="LAF92_001719"
     CDS             complement(14637..14831)
                     /locus_tag="LAF92_001719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790739.1"
                     /translation="MVRSKLGYMHELPDPPDYHSNLGNCSLDPNLDRHDKSPMVVTIG
                     NWFYGASKRTKVQRILEVSA"
     gene            15493..16842
                     /locus_tag="LAF92_001720"
     CDS             15493..16842
                     /locus_tag="LAF92_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790740.1"
                     /translation="MSSLRDMEELIGDIVDVELKEYMREALICYMTGAHRACVVLSFI
                     AIFEDLFKKLDGMATTNRVAKAIFTEISKKRDEQKVYENDLLNKLKSEKIITEIDADF
                     LTVLRTLRNKAAHPSGHKPTAEEARYVYSETINRFLSKPVLSTTQVADQILSKLTNSY
                     LFPTSNIQDHAIVVNEGVKNLHHDGYSYLLKKLIESLDNANEQIKLNAKRYLLGLSFK
                     PLNNDVLEQIKKHFLVECSSDESKRQCLMECISTNGNLLKGMEDIVYLRLNKMFEDTI
                     STTKSSDQHTYLRHPVQIAISVLGLEREIIDRCFRSSIDDVLEKYKLSPVLMKNIKGH
                     EWARQKVIQSIFGQAGSTTFSEANTFAQSVNSYDGDISSLMDNINCFELIVHICAAGN
                     WGSFGAEGMMKGKFNNIPKIKEKAVKALQDNISNCQEIIARINPALCEAKDFEERYL"
     gene            17476..17631
                     /gene="hokD"
                     /locus_tag="LAF92_001721"
     CDS             17476..17631
                     /gene="hokD"
                     /locus_tag="LAF92_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI9790741.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            17848..18099
                     /locus_tag="LAF92_001722"
     CDS             17848..18099
                     /locus_tag="LAF92_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790742.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDSRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            18166..18444
                     /locus_tag="LAF92_001723"
     CDS             18166..18444
                     /locus_tag="LAF92_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790743.1"
                     /translation="MARNVKYYNSENSPVLACTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            18446..19501
                     /locus_tag="LAF92_001724"
     CDS             18446..19501
                     /locus_tag="LAF92_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBI9790744.1"
                     /translation="MRVLLRPALVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMCN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVICAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            19502..19882
                     /locus_tag="LAF92_001725"
     CDS             19502..19882
                     /locus_tag="LAF92_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI9790745.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQWLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVSGGRLGVKIYEIMHDGQVQK"
     gene            19879..20700
                     /locus_tag="LAF92_001726"
     CDS             19879..20700
                     /locus_tag="LAF92_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBI9790746.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASVSTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCGGKKFIESVVFTNKVQHPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVIRFDIEEAWAERQLKRVTR"
     gene            21136..21351
                     /locus_tag="LAF92_001727"
     CDS             21136..21351
                     /locus_tag="LAF92_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBI9790747.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
     gene            21356..21700
                     /locus_tag="LAF92_001728"
     CDS             21356..21700
                     /locus_tag="LAF92_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HBI9790748.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLEVAAGLCEGDEQSPEKRPVILDAQNVWITYKGKLPG
                     RITGSLKTPPKW"
     gene            complement(21666..21938)
                     /locus_tag="LAF92_001729"
     CDS             complement(21666..21938)
                     /locus_tag="LAF92_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790749.1"
                     /translation="MDNNTISLQELLDCISRLRDDVNALTVAFSYLALSIPREQMQPT
                     LASLLLESRNPKWSQEQQNSFKWLVALLEEKYAGKITISVESSENQ"
     gene            22044..22586
                     /locus_tag="LAF92_001730"
     CDS             22044..22586
                     /locus_tag="LAF92_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBI9790750.1"
                     /translation="MNAKIKYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLTKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNADDRKGACEAIRWWIKDGGRDCRLTKGQKNGC
                     YGQVERRDQESALACWGLDQ"
     gene            22583..23119
                     /locus_tag="LAF92_001731"
     CDS             22583..23119
                     /locus_tag="LAF92_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790751.1"
                     /translation="MKINPDLIGVVVIAGLSVALVKSCSHASNLQSDNDVLRSDNSML
                     EQVIATQAFNFNRFNQVAEHANSLNSLIDTSTEKTVIEYREILRREKTCDLPVPADIA
                     GGLLEYTYRLRASAMHTDTGRPNEAYDRTAATSSMTYCQAVLWIKPLLALIEKGNNNF
                     SSIREIDELRYRPSERGQ"
     gene            complement(23288..23980)
                     /locus_tag="LAF92_001732"
     CDS             complement(23288..23980)
                     /locus_tag="LAF92_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790752.1"
                     /translation="MSREGSLDLLQEVEESLDIMRQTQHIKPVKVKAILENLRSSLEY
                     LANDTYDKYISPSSNDERPKIYFPYRKKEFIDKFFSKTLKINPPSSSPLYKIFTSIQD
                     YHTGESWLEMMCNLTNEVKHRQPIPLREESSVKELSVSVDGFNLITAGSSAKILFKNN
                     HINGKKLEDFTFENGRLQRSGNGVPLNIVITEEKKIRFHGNDYEVIPFIESCLIKIRI
                     FIIEAYNELDKI"
     gene            24552..25046
                     /locus_tag="LAF92_001733"
     CDS             24552..25046
                     /locus_tag="LAF92_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HBI9790753.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTVAGADDEEEL
                     QQEE"
     gene            25046..27148
                     /locus_tag="LAF92_001734"
     CDS             25046..27148
                     /locus_tag="LAF92_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBI9790754.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRILPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPSPEPVPTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            27145..27357
                     /locus_tag="LAF92_001735"
     CDS             27145..27357
                     /locus_tag="LAF92_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790755.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            27285..28865
                     /locus_tag="LAF92_001736"
     CDS             27285..28865
                     /locus_tag="LAF92_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011703754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBI9790756.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAFLDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKENERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDGY
                     LILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKE
                     AEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGS
                     SAATKRQEPQHTDDQSEE"
     gene            28810..30840
                     /locus_tag="LAF92_001737"
     CDS             28810..30840
                     /locus_tag="LAF92_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBI9790757.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLVDETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAAPVVDENSIRAQVLAEQKARVNGINDLFAMF
                     GGRYQSLQAQCLADPECSLEQAREKLLNEMGHESTPSNKNTPAHIYAGNGNFVGDGIR
                     QALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDF
                     GNILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEY
                     KYVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAI
                     LTSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFV
                     LVPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASK
                     GSDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            30927..31250
                     /locus_tag="LAF92_001738"
     CDS             30927..31250
                     /locus_tag="LAF92_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HBI9790758.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTNIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            31243..31518
                     /locus_tag="LAF92_001739"
     CDS             31243..31518
                     /locus_tag="LAF92_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HBI9790759.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            31530..32108
                     /locus_tag="LAF92_001740"
     CDS             31530..32108
                     /locus_tag="LAF92_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9790760.1"
                     /translation="MKGLENAIRNLNSLDTHMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            32105..32506
                     /locus_tag="LAF92_001741"
     CDS             32105..32506
                     /locus_tag="LAF92_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9790761.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            32518..33261
                     /locus_tag="LAF92_001742"
     CDS             32518..33261
                     /locus_tag="LAF92_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9790762.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPHLAEEGTETVS
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVAAVAVSAAG"
     gene            33322..33708
                     /locus_tag="LAF92_001743"
     CDS             33322..33708
                     /locus_tag="LAF92_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBI9790763.1"
                     /translation="MFLKQGSFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            33717..34046
                     /locus_tag="LAF92_001744"
     CDS             33717..34046
                     /locus_tag="LAF92_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBI9790764.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFQMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            34018..37083
                     /locus_tag="LAF92_001745"
     CDS             34018..37083
                     /locus_tag="LAF92_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBI9790765.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTGVLTQAEAEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQISALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNELQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINSMQYADQAVRDAAIQRENERYEKALASGKKKTRDPRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLRFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            37083..37412
                     /locus_tag="LAF92_001746"
     CDS             37083..37412
                     /locus_tag="LAF92_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9790766.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSIY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWITITA
                     DFEQVVA"
     gene            37422..>37950
                     /locus_tag="LAF92_001747"
     CDS             37422..>37950
                     /locus_tag="LAF92_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBI9790767.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWT"
BASE COUNT         9706 a         8412 c         9897 g         9935 t
ORIGIN      
        1 aaccacgaaa agtaacgaca aaacattatc tgtcagcgcc acgccatcaa cggatccgat
       61 caaaaaatta gattacacag cggttacttt actgaataat caacgagttg gtaatgtaaa
      121 acagagctgt gaatatgaca gtcacgccaa tccggtggac tgtcagctga tcattgttga
      181 tgaaggagta aaacccgccg tcgaacgggt ttacaccatc aaaaatacga tcgattatta
      241 ttaatgctat tgtgcggtcg gcttcaggag agtctgaccc ggtgttttgt gctctgccag
      301 atactgatgc tggaatatac acatgcgaat ggcattacga tattgaccat taataaagaa
      361 ctcgtgcatc aattcacctt caaccgtaaa gccaagcttg cggtaaatgt gaatcgcttt
      421 ttcattctct ttatcgacga tcagatacag cttatagaga ttgagaacgg tgaagccata
      481 gtccattgct aatttggcgg cacgggttgc cagacctttc ccctgatact ccggggagat
      541 aattatctga aattctgccc ggcgatgtac atggttaatt tccaccagct ccaccagacc
      601 ggctttttcg ccgtcacatt ccaccacaaa gcgtcgttcg ctctgatcgt gaatatgctt
      661 atcatacaga tcagagagtt caacaaaggc ttcgtagggt tcctcaaacc agtaacgcat
      721 cacactggcg ttattgtcga gttgatgtac atagcgtaaa tcttcacgct ccagcgggcg
      781 tagcttaaca ctgtgggcgc ttggcataac gtgtccttac attccttaaa tcaataacag
      841 gttagggggt aataacgcgg ccagttcgac ggtccaggca gcgcaaagta tttggctccc
      901 agtaggcatt gatgttggcg ctttgctcac atttatcgcg gttatcaaaa gcggcgtcgg
      961 ctttatccca ctctttttca gtgcgtttat tcactttctg acgcagattg cgcgtgtcat
     1021 tccattgctc tttttccata gcggcgtgct ggcggctttg tgcactgtcg ccagactcaa
     1081 tcaccagttt gttagtttcg gcatgaacag ttgtgctcag tgccagtgcg caaggcagca
     1141 gaaaagcgag caggccgatt cgtttgctga gagtgatttt cataattcat tccctgtatg
     1201 aatgattaaa ggagattcta caccatccac tgcgaacgca aaacgtacca ggagggtgtt
     1261 tatattgatg atattatgtc gccctataac tatacatgat gtcaataagt gacaaagatg
     1321 attaaaacaa cgttactatt ttttgctact gcgctgtgtg aaattattgg atgctttctg
     1381 ccctggttgt ggttaaaacg aaacgccagt atctggctgt tgcttccggc ggggatttca
     1441 ctggcgctgt ttgtctggtt gttaacgttg catccagcgg cgagtgggcg tgtttacgcg
     1501 gcttatggtg gcgtttatgt ctgcacggcg ttgatgtggc tgcgcgttgt ggatggcgtg
     1561 aaactgactc tttatgactg gacgggtgcg ttgattgcgc tttgcggcat gttgatcatt
     1621 gttgcgggct gggggcgcac gtaggaacat aaatccattt tatcaataag ataagaggaa
     1681 gtgtcagctg acaaaaggta ttctatttca tcttttgtca accattcaca gcgcaaatat
     1741 acgccttttt ttgtgatcac tccggctttt ttcgatcttc atacttgtat ggtagtagct
     1801 cagttgcgta gatttcatgc atcacgacaa gcgatgcaag gaatcgaaca tgaagatcgt
     1861 aaaggctgaa gtttttgtta cctgtccggg gcgtaatttc gtcacattaa aaatcaccac
     1921 tgaggacggt attacgggcc ttggggatgc caccctcaat ggacgtgagc tttccgtggc
     1981 ctcttatttg caggatcacc tttgtccgca gcttattggt cgcgatgcgc accgtatcga
     2041 agatatctgg cagtttttct ataaaggtgc ttactggcgt cgcggtccgg ttacgatgtc
     2101 ggccatttca gcggttgata tggcgctgtg ggatattaaa gccaaagctg ccaacatgcc
     2161 gctttaccag ttactcggcg gcgcgtctcg tgaaggggtg atggtttatt gccataccac
     2221 cggtcacagt attgatgaag ctctggatga ttatgcccgt catcaggagc tgggattcaa
     2281 agccatccgc gtgcagtgcg gaatccctgg tatgaaaacc acctacggca tgtcgaaagg
     2341 taaaggtctg gcttatgaac ccgcaaccaa aggacagtgg ccggaagagc agctgtggtc
     2401 gacggagaaa tacctcgatt tcatgccgaa attgtttgac gcggtacgta acaagtttgg
     2461 ttttaatgaa catttgctgc atgacatgca ccatcgctta acgcctattg aagcggcgcg
     2521 ctttggtaaa agcattgaag attatcgcat gttctggatg gaagacccga cgcctgcaga
     2581 aaaccaggaa tgcttccgtc tcattcgcca acataccgtc acacccatcg cagtgggtga
     2641 agtcttcaac agcatctggg actgcaaaca actgattgaa gagcaactca tcgattatat
     2701 ccgcaccacg ctgacccatg caggcggaat taccggtatg cgccggattg ccgattttgc
     2761 ttcgctgtat caggtacgta ctggctcaca cggtccttcc gatttgtcac cagtctgcat
     2821 ggctgcagcg ctgcactttg atctgtgggt ccccaatttc ggtgtccagg aatacatggg
     2881 ttattccgaa caaatgctcg aagtcttccc gcacaactgg actttcgata acggctatat
     2941 gcatccggga gacaaaccgg gtcttggcat cgaattcgat gaaaagctgg cggcgaaata
     3001 tccctatgaa cctgcttatc taccagtcgc acgtctggaa gatggcacgc tgtggaactg
     3061 gtaaggagta agataatgaa aagcatatta attgaaaaac cgaatcaact ggcgattgtc
     3121 gaacgtgaaa tacccacccc gtcagcgggt gaagtacgag taaaagtgaa acttgccgga
     3181 atttgtggtt ctgatagcca tatttatcgt gggcataatc cttttgcgaa atatccgcgc
     3241 gtcattggac atgaattctt tggcgtcatt gatgcggtgg gtgacggcgt ggaaagcgcc
     3301 agagtcggtg aacgtgttgc tgtcgatccg gtggtcagct gtgggcattg ctatccgtgc
     3361 tctataggta aaccgaacgt ttgtacgaca cttgctgtat taggtgtgca cgctgacggt
     3421 ggtttcagtg aatatgccgt ggttccggca aaaaatgcgt ggaaaattcc tgaagcagtg
     3481 gccgatcaat atgcggtgat gatcgaacct tttaccattg cggctaacgt aaccggacat
     3541 ggtcaaccga ctgaaaatga taccgttctg gtttatggtg ccggtccaat cggcctgacg
     3601 atcgttcagg tattaaaagg cgtctataac gttaaaaatg tgattgttgc cgatcgcatt
     3661 gatgaacgac tggaaaaagc gaaagagagc ggggcagaca gggcgattaa taacagccag
     3721 acaccgcttg gcgagatttt cgctgaaaaa ggcatcaagc cgacattaat tatcgatgcg
     3781 gcttgtcatc cttctatcct gaaagaggcc gtaacgctgg cttctccagc ggcacgtatt
     3841 gtattgatgg gcttctccag tgaaccgtct gaagtgattc agcaaggaat taccggaaaa
     3901 gaactctcta ttttctcttc acgcttaaat gcaaataaat ttccggttgt tatcgactgg
     3961 ttaagtaaag ggttaattaa accagaaaaa ttaattaccc atacgtttga tttccagcat
     4021 gttgctgatg ccattagttt atttgaacag gatcaaaaac attgctgcaa agtcttactc
     4081 actttttctg aataatacca ataacggcga gtaagtagta cgcatcttac ctctttttta
     4141 gagataacca ttatgacaat agaaaaacat gaaagaagca ctaaggattt ggtgaaagca
     4201 gcagtatcgg gatggctggg cactgcgctt gaatttatgg atttcaagag tcatgcgcgt
     4261 taactatttg ataaatatta aattaatttt tcattgcttc gttatggggc atggttgggg
     4321 caaactcgct taactgtgta tttaacaaag ctacctgtgc attattgttt tcagacatcc
     4381 attttccgta tacctgaaat accatttgcg catctgcatg gcccatctgg tttgctataa
     4441 atgccgggtt agcaccagct gtcagcgacc agcaggcata agtatgtctc gactgatatg
     4501 attttcgatg gcggagtccg gcacgtttta tcgctgcgtc ccacatctgc cttattgagt
     4561 caacggtaaa atggtcacca taatttttta ctctcgctga cacttcaggt tgaaaaacaa
     4621 aggtgcattt ttgtttttct gttctgccat actctctgag gtgaacatca atgatatgct
     4681 ctttactcag tctcgttaat gtcatctgac tccggagagc gtcgattgct ggcttaataa
     4741 gatgaatgac ccgattggtt cccgcctgtg tttttggtac cgtgaaacgg tcttttgcta
     4801 aatttctcct gatcatcatt gttccatttt tcagatctat gtcctcccat ccaagtgcac
     4861 acagctcacc agggcgaacg ccagtataaa cagaaacaca ccataaattt tttgcttgct
     4921 gatttctgca cgcatcgata agacggataa attcctcccg cgaaagagga tccggaatgg
     4981 ttcttgattc ctttaatggc gagatcccct taaacggatt atctgccagg taaccgttat
     5041 caacaccaaa ctggaacacg gcgttaagat ttgtcatgta attatttaca gttacagccg
     5101 atctccctgg ttgtgtaaca atatagttac ttttggggat ctggtatcca gtcagtagct
     5161 ctttacgaac ctccagtaat ttttctttat taatcgatga ggcaagattt ttttcaccga
     5221 ttatgctcag gatatttttg atgacggcac ggtatgtgtt gagtgatgtt ttggcgactt
     5281 cagtttcttt cagtgccaga aatttttcag ccagttcttt tatggttaaa tcttgtcggg
     5341 cctcaccaaa tttttccaga ttgcgtgagg agggaaactg ttttgcatag tcgaaaacac
     5401 cagtttttat tgcgtaacaa acagaggagc gtagttcacc tgcaacgcgc ctgttttttg
     5461 ctgtgtcagg aacccccaga ttttccctga ctcttacgcc tttataaaca aaccagatac
     5521 gtaatttccc tccatggttt tccacgcctg tcggatattt catttcaact tctctcatta
     5581 gttagtgtgg cttttagtca agtaagatga cgtcttggtc tcgctgatgc ctggcgctca
     5641 atccagcgat caatttcttc caggttgtaa aagcatggac tgttatccca tggcataccg
     5701 tcatgagcga catgcttata ttcccttcct tccataaacg atttttcccg ggcctttttt
     5761 aacgtacctt tttttattcc tttcagcgca attaactgct cttcggatac ccatttgccg
     5821 ggagagacaa tcatgattac ttcgctcatc gatttcttta tctcttacat cagacgagcg
     5881 ccggttgcag aataccagtc acaaccggcg acagttgaac attaagaatc agcctgactc
     5941 gggatcagtt tttgccagat aactgaaacg tattttgcct ggtaacgggc gtcatcaagt
     6001 gcattatggc gctcaccttc gaatggaata gccgttctgg catcgaagtc tatggctttc
     6061 cccagctcaa cgattgtgcg tacatcgcga tcgttgtagt aacgccacgg gcaggggatc
     6121 ccctgccgtt cgtatgaacg gcgcaaaatc gtgttgtcga agttggctcc atttccccaa
     6181 acctgaacaa aaaattcacc ggagttttcg tcgataaatt cccgcaattg taacagtgca
     6241 tcatctaacg ggatttcatc ggtcataatg gcagattgcg cttcgcgtga ttgcttaagc
     6301 caccatttaa tggtgtcccg atcaatgact ccgccagcag tttccagatc gatagtctta
     6361 ctaaattccg gtcccatatc tccggtttgc ggatcgaaaa atattgcacc tattgagatg
     6421 atcggggcat caggattttt tcccatggtt tcaaggtcga tcattagatg gtcacacgtc
     6481 ctgctggtgg atgtgatttc gtgatgaccg ttcaccttaa ttgggtgatc tgccgtctcg
     6541 ccagtttcat tatcgctatt gtgatgctga ttgccgccag tgttctcctt gtgtggatgt
     6601 tcagcgcctt ccatttcctc cggatcatct tcctgaactt caacctgata ctcttcatcg
     6661 aatgtttcct ggtatgttgc gtcgcccatc accgcgccac aatcagggca gttgccgctg
     6721 ccggtctgac cgcaggcggt gcagactttt tccacttcct gttgcgccac tggttcaggc
     6781 tgtttcgttt ctggctcgtt ttgtaacgca tttggactgt tttgttccgc tttttggtag
     6841 ttccgttccg attcatgctg gttctggttc acagaatcgc gggtctggat ccccttaacc
     6901 catttcggat cattcgggtc actaatccct tcaacaaatt caccacgtga tgcagcaagc
     6961 aacttatcgg cgtcaggctg gctgatattg gctgcctgca taattttgtt tacttcgtca
     7021 gcggtaactt ttatcggctc tggttgttct aaatcttcag cggtatctac attttgcggt
     7081 aagcccgtgt atgtgccatt ttttcgggca aaatattctt cttttgtgat ttcagtggcg
     7141 ccagcagcca gtgccttatc cagaccagaa agtttgtttg cgcgaccgta tttttctccg
     7201 tccttatctg cgaagaggaa atagaacggc ccctcacgct ctacagatgg ttcagcttcc
     7261 ggcgcggttt cattttttgg gatatcagat acctcagttt ccactgcatc agtttgtgtt
     7321 tctgatgact ggagaacatc aacagtgccc aggtctgttt cttcattctc aaacacgccc
     7381 tttgtcgtca ggtattcgca gatatatttg ttcagtgcta cgggatcttt gtgaatgtcg
     7441 atcggacgct cacggacaag gccaaaaata gtttggcggt cgtagcgaag ggcatctggc
     7501 tgtttgcgca ttgatgccga gatacgcttc cagtcttcgc ggtcgttgtc gataacttct
     7561 ttttttgccc agcgatggat gctgccgtca atgtttccgg catccacatc accaggccag
     7621 agagcgtagg ccagttcgtc atccagtgtt ttccatgtct gcttgtattc gcgatgaaag
     7681 gcagcaatga ccgggctgat ttttcctgtt gaattttcag tgtactgttg attggctctg
     7741 gcgcgggcga gatcaacaac agacgtgtat tttccggttt ccttgcgttc accttcgcga
     7801 cgttttttcc agatgcgcat ctctgcctga atttcgggcc atttagcact aggaatacat
     7861 ttatgcttaa cccacccaat ggcgtgcaac ttaagctccg gatacatggc gttaacttct
     7921 ggcattttca tcaacgcttc aacgatatgt ccttcgaatg ttgccatgtc ttcctgcaac
     7981 aattcctgtg cgcttataac catatcaacg gtgatgtttt cacatgtgtc gaacttaacc
     8041 atgacagcgt tctgtacttc aggggccagc ttgtcaaaag tgacgttcat cggatcggat
     8101 tcagtctcaa ccgggacaaa agaagcagac tcctcatccc agcggttttc ctgcatatat
     8161 tcagcatccc atgaatcgag ggcagggcgg ggtatgccag gtttatcctc gcagacaata
     8221 aatttataag cgcagtcctg agcagccgga taatgttcca ggaattgcca gtgaaatttt
     8281 gctcgagcac ggcgttcgtc gccagcttca atggctgtgg ctacagccac agcgccttct
     8341 tcccttgttg ccagttcgtc aggaatagcg gcgcaaataa agactttact cattttgttt
     8401 taacctcatg acagatttaa ggatgaacaa atccctgcca ttgctggcat ataagaatga
     8461 aaccggatat ttattacgga actgttttaa agacctgccg ggatttcgat attatcctgg
     8521 tgaataactt tatcgaccgg gtaacagtta ccgggaattt tctgttcggt tgctgcagtc
     8581 atacactcct gcattgtcct gtgaacactg actgcaatat caactggcac tccggaaaca
     8641 agaaaaactg tcagaacaag cgcaaatgct gaattcattg tgcacatcct tttggcatca
     8701 gacgtaaacg agccagcatt gaaacaatgc atattttatt taatagctcc cgttcttgtt
     8761 ttctcttgtt aatggcatct tcagtaaata cagggttact gatcgtgaca ccaatttcaa
     8821 aacaaccttc agacgtatta acgtttggta ataacatttt cattatcgcg tcctcaacaa
     8881 tgaattttgt gatgcagtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg
     8941 tcggatacag agaatccacc cataacactg tttttggttt taactgttcc gcgtgcgctt
     9001 agccgcattc acagcatcac aaaattcact ttaaaaacgg cggcagagca gtcacggagt
     9061 aaaactgata ccgccaaacg tcaccagaaa attgataaca gagggcgttg cagcggggtt
     9121 gtcacttaag cgtatggtca acctgacaac tcggtgtcct caacggggaa ggaataaccc
     9181 cgccatactt accgccgcgc cattttgcgg gttgccacaa ccagaagcgc acggtcgaat
     9241 taaatttaac gacaccgtac agtgagacga acttcgccgt gcgctttcgt gttgtgtgcc
     9301 tgcttttaac cacgtcaggc gaggtggtat ccttaaaatc accacagttt taaggattca
     9361 ttaagcaatg tcgcaaccac caataaatcc gcttaagaac atgaaaattg attactggta
     9421 taaagcgctt acagttgttg gcgctgcgtt gtttgtcttt aatggaacgt ctttttttga
     9481 cagatatccc gttgttccat tgggtttttt gtcctccggc atctttttta ttggtttggg
     9541 ggagtggatt aatcaccctc tcaaagtgag atttattggt cctggagttt ggactcgtgg
     9601 atataatcgt tcttcgtgcg cactcggtat catcttcgac atacttggtt gtttcctgat
     9661 tgttacagga gtcgtcaagt tcttctgatg taaaaccgca aatggggcac gtaacgggaa
     9721 ttttgaaaag cgtttctccg ggttccagaa caaaattttc tgcggtctga ttttgcttct
     9781 catatttgtg ctccgcgtca ttgtgagagc acattcttat tctgagtgcc tgtttaaact
     9841 cactgaagct gagagcttct tcgccttcgg caaggccttc gaagtattct tcgtaagcct
     9901 tttccatgat tgtgtcgaaa tccatatcac tcacctgagt ttctttccag ccagcgacgg
     9961 gcaccatttt cggttttaaa cgttttgctt ttggtatacg tcatcgcggt gaacgtgccg
    10021 tcctggttgg ggaacacgcc acataccaga gattcgctgt tgccaagatc gatagtatcc
    10081 atgctgacct catttcccct taacgccggg gtagcggaac tgtttgctga gaacaccgtg
    10141 cggtgtcttg atggaggtaa tttagttttc tcattatttt tcgtcaaggt tttttgatga
    10201 gaaaactcaa gtattggcgc aagataaagc caatacattg aaatgtaagg ctttaaaatt
    10261 ttgtgaaggg atgattattg atgcttgttg cgtttgcgag cctctaatag ctcggtgaac
    10321 aggcgattaa aattctcaac gcgggcgcgg agttcgctga gttgtgcttg ttgctctgat
    10381 ttaggaagtg cgcgatacaa acgcagcatc tctaactcat cttccgataa gtctaaggca
    10441 ctatcgagtg aaacaggggg agctggtgtt ttgtcctcgt cgccaaacag tatccaggtt
    10501 ggtgaacatt gcaatacctc ggcgaggcga tgcaaatttt gcccgcgcgg ggctgtatgg
    10561 tcactttccc ataatgaaat tgatgagcca gatacgccag cagctttgct taaaccgttt
    10621 tgacttaagc ctacctgctt acgtctttct ctaatgcgtt gacctaaagt tttctcgttc
    10681 atatttagat atcttaataa cccttgactt gagattcctt gagtgattag cattgagaaa
    10741 actcaatatt ggaggtgcga tgtttaaatc agacgtaatt aatttttacg gtacgaaagc
    10801 caaagtagcg aaagctgctg gcgttgaccc atctgctgtt tctcaatggc aagagctggt
    10861 tcctgaaggt cgcgcgatgc gtctacagga ggcatctggc ggcgagcttc tgtatgatcc
    10921 caaggtttat gacgaatatc gtaagacgaa gcgggcgggg cggttgaaca atgaaaatca
    10981 ctcctgaaca ggctcgtgag gctctggatg cctggatatg tcgaccagga atgacacagg
    11041 agcaggcgac gatattaatc actgaagcat tctgggcttt gaaagagcgc ccgaacatcg
    11101 atgttcagcg tgtcacatat gaaggtggcg cggttgatca gcgagcgctt agcgttaatc
    11161 gagtgaagat attcgaacgc tggaaggcta tcgacaccag ggataagcgt gaaaagttca
    11221 cagcgctagt gcctgcaatt atggaggcta tccggattag tgatttcagg ttgtatcgtg
    11281 agatcagtga tggaaaaagt attacgtaca tgatcgccgg gttaaataaa gaatatggcg
    11341 atgtggtgga atccggactg ctttttgcag atcctgccgt tgtagatcgt gaaactgacg
    11401 aacttataga aaaagcaatt gctttcaagc ttgcgtatcg acagcaatac caacaaaaag
    11461 ctggatggaa ttatgagtct tctttttgct gaacgcccac tggttataaa cacgcagctg
    11521 gcaatgaaaa tcggcttaaa cgaagccatt gttttgcaac aactgcacta ctggttgaga
    11581 gataccaact ccggtatgga atgtgatggt gttcgctgga tttataacac aacggaacaa
    11641 tggctggaac agttcccatt ctggtcagag tcaacgttaa agcgcgcgtt tgcaagtctg
    11701 aaaacgctgg ggcttttgcg ttgtgaaaag ctcaataaat caaagcgcga tatgaccaat
    11761 ttctacacga tcaactatgg gagcgagctt ttagatggtg gcaaattgaa cgaatccatc
    11821 ggttcaaaat gcgccgctcc atcaggtcaa aatgacacga tggaagaggt caaaatgaaa
    11881 cgctccattg gttcaaaacg acccaatgtc atcgggtcaa aatggcccga tgatcctaca
    11941 gagaatacaa cagagattac tacagagaat aaaaacactt ttagtccgga agcctcgcaa
    12001 ccggacccgc agacggctga acaggatttt ttaatccggc accctgacgc agttgtgttt
    12061 agcacgaaaa aacgccagtg gggtagccat gaggatttgg cgtgtgcgca gtggatctgg
    12121 ggacggatcg tgggtctcta cgaacaggcc gccagtgatg atggcgagat catgcgacca
    12181 aaagagccta actggactgc ctgggccaat gacgtgcgta caatgcggat gctggatggc
    12241 agaagccaca gacaaatttg cgaaatgttt ggtcgggttc agcgagatcc attctgggta
    12301 aaaaacatca tgagcccgtc aaaactccgt gaaaaatggg acgaactggt catccgcctg
    12361 ggacgttcac ctgtacagcg ttgtgtgaat catatttctg aaccggatac agaaattccg
    12421 cctggtttca ggggataagt gttgatttca ggtcatgagg taattttaag ggggacttgt
    12481 ggcaaaagtt tttacacaag aagagcggga aaaaattaaa gggcaggtgg tggaactcgt
    12541 gcgccagagc ggtcgtgaga cgttacggca actggaagct aaaacaggtg cgacaagata
    12601 tctgatgagc gttcttgcca gagagttggt tgccagtggt gatgtataca actccggcta
    12661 cgggttattc ccgtctgaac aggcgcgtaa ggactggcaa aacgcccgca aaaaactctc
    12721 gagggcaaag gtgaagaaac ctgctgtggt tgatccggat cttatctggt cattatctga
    12781 cggagaaata cgtcgctacg acagtcgtct aaacataatt tgtagcgagt gccggaggag
    12841 tgaagttatg cagcgcatac tggcatttta tcagggaaat gttcgatatt ttagacgtta
    12901 ctagattaaa gagcattagt tcagatatga attgacattt tcatggcaca gggttgagct
    12961 agcgtggttg tccgctttgt gccagtaacg gacgttttaa acatttgtaa tcactaatcc
    13021 gcgtagaaaa gatctcaccg cataatcagg caaacatgca ggaacatctg aggagttttt
    13081 tcgagatacg gacaaggaaa agcagcagaa cctcgttgag gccctctcag gtggtgccgg
    13141 aaagagatgt tatggtaaat atcgcagaca tagagcattt gaagtgatat ttagcatact
    13201 tatgctctta ttgtagattg gcttttacca aataagcttt ttactacaat gtatgaacat
    13261 aagccgattg atctttttag gcagcagtcg cttgctcact tctgctatca ccaaacatag
    13321 tctgtgctgc catgtcaaca tctccagata ctgaattctc gtatcttcta atagcttcat
    13381 ctggcgtttc ggtaaaggtt ttttcagcaa aaataatatc ttgtagttcg catatcttta
    13441 ttttcaactt tcttagggtg tcactatcgt tttttcctgt gatcctcaca caagcattaa
    13501 tacgattaat tattatttct tggaatatgt ttttcttttc ctcaattatt cttctaaaaa
    13561 gaactattgt tttttctttt cctataatat cctcttcaag aggcgctttt gctttctcta
    13621 tatctttctt tgacgcaaac tcattaaatg caaaagtggt tatgtaacct attattatag
    13681 gaaggaatga ataggaagtt ggtacttttg ttgttaggta ggttgatatg gctcctatga
    13741 ctattaatac tgttctttta taccaaggta cagagattat gaattcgaaa aaacgtgtta
    13801 tcgccacatg catcttttgc aaaaggcaaa ggattaaaac tactataaca acgataataa
    13861 gtatcgcagc aaaacttgaa gcgacctcag ttactgatga attactattt ttttcagcag
    13921 tatagctact tacagtagat ggcgtattga tttctaattg tttgttttca atctcggtgc
    13981 ccacgctaat cccttaatct ttggccattc ctaatttttc aaataatgtc ggtgctccag
    14041 ctaaaattgc ggctagcatt cttaacgaat caccagataa gttgagcatc ttttcatcaa
    14101 cagcaatctc ttctactgtg aaaacaatca atggtgtttg ttcttcttca attattgtcg
    14161 attttattcc tattgatttt ctattaaaat tattagcaat ggacagtagc gattgtttat
    14221 ctttgcctat tttcatggcg ttggtgttaa aagagttcat cagtctcatt actattattc
    14281 tagatgctac tctcttctca agtattaaat tgacttcatt gaatttaaat gcaaccaatt
    14341 caaatccttc ttctctctct aattctttta tattattaaa ctcacttatg ctagatataa
    14401 tatctataag gctttgatta tttctctttt gtgtttcggt ttttttcctt cttccagaag
    14461 acatcttttt tccttaatga atttatgtgt ggtgatagta tccagctcta gataagaaaa
    14521 aacaccgcta ccaatggtgt tttttcaagg ttcttagagc taccaacttt ttgaaccgtg
    14581 gtaactggct ttaacggagc gcagtaacca aaactgtctt ttcagtgtat ctgtagttag
    14641 gccgacactt caagaattct ttgtactttc gtgcgtttac tcgctccata aaaccagtta
    14701 ccaatggtta ccaccattgg cgatttatcg tgcctgtcaa gattgggatc aagagaacag
    14761 ttacctaaat tgctgtggta gtcgggtgga tcggggagtt cgtgcatata gccaagcttg
    14821 gatcgcacca cctttaagga aacccgttgt acggaaagga atgggaaaac gcgaccttac
    14881 ggcggtctgc gacgggtaaa cctcatggtg ggaacagggc accgctttgc tgagatgcag
    14941 gatagggcag acccccgcaa aatgtcgtca tttgcggtgt ctgccccgaa aacagaatat
    15001 tttttcctga gcctgcagct ggctctaaaa accgctatcg cctcacaaag ctcgctgctt
    15061 aacgcctcac gttcacccac taaacagcgc acctgttaac cgtgaatcac gtacagattt
    15121 atcaatatac ttcgcacgtt tactgccgga aatcctccct taattcccgc atttgcccga
    15181 aaccgcccgc cattcacccg cgtgcgcagg cattcagatg cgcagaaatt agcatgggtg
    15241 ttttttcaca atacggacgg ggaatagtgc cggaaacggg ccggacgcgc aaagatcgcg
    15301 ctgagtgggt gcggccaggt cttgttgagt ttaatgtaat agtaaatcca gtctttatga
    15361 gtgaaacgtc cacttctcgc tcatggcggc actttagtgg gcctgtaaag tggatgttgc
    15421 caatcaacga tgagcaaggc attctaattg cttgaggtgt tttcgtataa aatttaaaca
    15481 aaaggagcaa ttatgtcgag tctgagagat atggaagagc ttattggtga tatcgtggat
    15541 gttgagctta aagaatacat gcgagaagca ctaatttgct atatgactgg tgctcatcga
    15601 gcatgcgtag tgctttcgtt cattgccatc ttcgaggact tattcaaaaa gttagacggg
    15661 atggccacta cgaatagagt tgctaaagcg atctttacag aaatatccaa gaaaagagat
    15721 gaacaaaaag tttacgaaaa tgatttgctt aataaattga aatctgaaaa aataattaca
    15781 gagattgatg ctgactttct cactgtgtta agaactctca gaaataaagc tgcacatcct
    15841 tcaggccata aaccgacagc tgaagaagct cgatatgtgt attcagaaac tataaataga
    15901 tttctttcaa agccagttct ttcaacaact caagttgccg accaaatcct atctaagctt
    15961 accaattcat acttattccc aacatctaat attcaggacc atgctatcgt agtaaatgag
    16021 ggtgttaaaa atcttcatca tgatggttac tcatacctcc tgaaaaaact catagagtca
    16081 ttagacaatg ccaatgaaca aataaaactt aatgcaaaaa gatatctttt aggcctatct
    16141 tttaaaccac taaataacga tgttcttgag caaataaaaa agcatttctt agttgagtgc
    16201 agttcggatg aaagtaaaag acaatgttta atggaatgta tttcaaccaa tggtaatttg
    16261 ctaaaaggta tggaggatat tgtttactta agattaaata aaatgtttga ggatacaatt
    16321 tcaactacaa aatcctccga tcagcatacc tatttaaggc accctgtaca gattgcaatt
    16381 agtgttcttg ggttggaaag agaaattatt gatagatgct ttagatctag cattgatgat
    16441 gttcttgaaa aatataaact tagcccagtg ctgatgaaga atataaaagg gcatgagtgg
    16501 gctagacaga aagtcatcca atcaattttt gggcaggccg gatctacaac tttctctgaa
    16561 gctaacactt ttgctcagag tgtaaattca tacgatggtg acatctccag tttgatggac
    16621 aacataaatt gcttcgaact cattgttcat atctgtgctg caggcaattg gggttcattt
    16681 ggagctgaag gtatgatgaa aggaaaattc aataatatcc ctaaaattaa agaaaaggct
    16741 gttaaagctc tgcaggataa tatctctaat tgccaagaaa taattgcaag aataaatcca
    16801 gctctatgtg aagcaaagga ttttgaagaa agatatcttt gatattcgtt ttaaaaataa
    16861 cagagggaat caatgactcc ctctttatag gagttattgg gtgggttaat ataacgttta
    16921 ttcaccatca atcaaccgca accttaatgg tgttattttc aaccgcgaac ttccgcttct
    16981 cgctcaaagc ggactaaaat gttggcttgc gccggacttt gcgtatttaa ataagtgctg
    17041 gtggtgactg gttgctgtgt tctatttcta cagaacaaaa ttacagaaac tatacccaat
    17101 agttgtattg aatcactgac gagacagcct catatttatc aggacaggtg tacgttctat
    17161 acaggaggtt gccgtgctgg ttctcaaatg tgcgctggct attgtggctg taatggcgat
    17221 ttattgtctt gccattattc ttatggatcg cctttctgac tgatttcata ttggcgaggt
    17281 aacgggagtt aagtagaatg gctgcgggtg cttgaggctg tctgcctcgg gcatgaacac
    17341 caacggcaga tagagaaaag ccccagttaa cattacacgt cctgcaagac gcttaacatt
    17401 aatctgaggc cacatctata ctttgcatac gtagattagc ctcttaccga ccaaaaggtc
    17461 aaagagaagc gggctatgaa gcagcaaaag gcgatgttaa tcgccctgat cgtcatctgt
    17521 ttaaccgtca tagtgacggc gctggtaacg aggaaagacc tctgcgaggt acgaatccga
    17581 accggccaga cggaggtcgc tgtcttcaca gcttacgaac ctgaggagta agagaccagg
    17641 cgggggagaa atccctcgcc atctctgatg agtcaggcat cctcaatgca cccacactta
    17701 acccgcttcg gcgggttttt gtttttattt tcaacgcgtt tgaagttctg gacggtgccg
    17761 gaatagaatc aaaaatactt aagtagcgcg cagggataag agggatggtc ccttaaaggg
    17821 gagagctaat tatccggaag gattctgatg atgaacatcg aagaactgcg taaaattttt
    17881 tgtgaagatg gcctctatgc tgtgtgcgtt gaaaatggaa atcttgttag tcattaccgc
    17941 attatgtgtt tgcgaaagaa tggggctgcg ttaattaatt ttgtggatag tcgagtgaca
    18001 gacggattta tcttgcgcga aggtgagttt gtcacttcat tacaggcact gaaagagatc
    18061 ggaataaaag caggcttttc agcttttgca gaagaataaa ctcatctaca atcttgcgcg
    18121 gggctgaact cccgctgagt aacaccgtgc caccggagaa aaccgatggc acgcaacgta
    18181 aaatattaca attctgagaa ttcgcccgtt cttgcctgca cgcacgagcg gtattctcac
    18241 gcattcaagt ctgaatggtt ccagcatcct ccatgcactg aagagcaggc tgaatggata
    18301 attcagtgtt accgcaggcg cggatacgag gttaagaaag cccttagcct tgactaccgt
    18361 cactggataa tctcagtcag actcccttac tccgaacgcc caccgcgccc atcccgcaca
    18421 ttccagcaac ggatctggag gtaacgtgcg ggtattactt cgacctgctc tggtaccaga
    18481 acttgggctg gtggtcctta aaccagggcg tgaatccatg caggtatttc ataatcctcg
    18541 agtgttggtg gagccggaac cgaaaagcat gtgtaatctg ccgtccgggg tcgttcctgc
    18601 cgttcgccag ccgctggtgg aagacaaaac attgctgccg tttttcagta acgcacgggt
    18661 gatttgtgct gctggtggtg ctggtgcatt gtctgactgg ctgttgcgcc atattaaatc
    18721 ctgccagtgg ccacacggcg attatcatca cagcgaaacc gtcattcacc gttatggtac
    18781 cggcgcaatg gtgttgtgct ggcactgcga caaccagctg cgtgaccaga catccgaatc
    18841 actcgagcaa cttgctcatc aaaacctgtc agcatggatg attgacgtca tcggtcacgc
    18901 aataagcggt acgcaggagc gtgaattatc tctggctgaa ttatcctggt gggcggtctg
    18961 caatcaggtg gcggacgcgc taccggaagc ggtattacgt cgttcgctgg ggttgcgtgc
    19021 ggaaaaaatc cgctcaatgt accgtgaaag cgacatcgtg ccgggagagc agaccgccac
    19081 cagcatactg aagcagcgca caaaaaatct tgcgccgctg cctcacgccc accagcaaaa
    19141 cccgccacag gaaaagacgg tggtcagcat tgccgttgat ccggagtcac cggctcagta
    19201 tctccagcgc cagaaaccac aacgggaaga gatgcctgta tacacgcgct gggtaaaaac
    19261 gcagaaatgc atgacgtgcg gtaatcaggc agatgatccg catcacatca ttggtcatgg
    19321 actgggaggg atgggaacaa aggctgatga tttgtttgtt attccgctgt gccgtaaatg
    19381 tcataacgaa ctgcacgccg gggtaaaaga ttttgaagaa aaacacggca gccagctgtt
    19441 gttgctgatt cgttttttaa tgcacgcgag aaattcgggt gtcctgaagt ggaaagcatg
    19501 aatgactgaa cgcatagaat ttgttttgcc ttacccgccg acggtgaata cctactggcg
    19561 acgtcatggc aatacgtatt tcatctcgga agccggaaag cgttatcgcc gtgatgtggc
    19621 gctaattgtt cgccagcagt ggctgaaatt aaacctgtcc ggaaggctgg cgataaagat
    19681 tattgcagag ccaccggata agcgccgtcg tgatctggac aatatcctga aagcaccgct
    19741 agatgcgctg acgcatgccg gactactcat agacgacgag cagtttgatg aaatcaatat
    19801 tgtgcgcggt cagctcgttt ctggtgggcg actgggcgtg aagatttacg aaataatgca
    19861 tgatgggcag gtccaaaaat gaaactggaa gatttaccga aatactattc cccaaaatcg
    19921 ccaggcctga ctgatgcatc cgtctcgacg tcaaaagatg cgctgagcat cactgatgtg
    19981 atggctgcgc agggtatgac acaaaaccgg gctgagatgg gattttctgc gttcctgggg
    20041 aaaatgggca ttagtatgaa tgacagggcg cgggcaacag aattactggc agattatgca
    20101 ctcagtcgtt gcgatcgcgt ggcggcgttg agaaaacttc cggcagaaat aaaaccggca
    20161 gtgatgcgca ttatggcttc gtacgctttt gaggattatg cccgcagtgc agcgagtaaa
    20221 aaacagtgcc cttgttgcgg tgggaaaaaa tttattgaaa gcgtagtttt tacaaacaag
    20281 gtccagcatc cggatggcaa gccgccagta tgggcaaagt gtacaaaagg cgtgtatccg
    20341 tcttactggg aagaatggaa aaaagtccgg gaagtggtaa aagttgcctg tcctgaatgc
    20401 gggggaaagg gtgaggtctc caccgcctgt aaggattgcc gtgggcgtgg tgtcgccatt
    20461 catcgtgaag agtcggtaaa acgtggtatg cctgttatca gagactgcca gcgttgtggt
    20521 ggtcgtggct atgaaagatt gccgtcaaca gaggcgttta atgccatatg caaagtgacg
    20581 agcgctatca ctcttgatac atggaaaaaa tcagtgaagc gcttttacga tacgttggtg
    20641 attcggtttg acattgaaga ggcatgggcg gagcggcagt taaagagggt aacgcgatag
    20701 tgttgttgat ttttcccgaa tctgtggtag attcgcctta acgatgggcg ttttatgcct
    20761 gacgttagag gcatttttac aacccgccgc cgagcgggtt tttttatctg cacaacaggt
    20821 aagagcactg aacccgcaga cctcgcggaa ttggtgaaag gtgccgcgca gtgctctttc
    20881 cgttgtggtg aatgcgcagg ctgatgcgcg aaagacattg cagctattgc ggaaaagagc
    20941 tgtccggcgg ggcaattaaa cgcccgtgag agtctgaaat aaccgcaagc cggggatcag
    21001 taccggccac cacgatctca aatgagctgt agtaactggc tgtcatgaat taatcagtga
    21061 cagttatgcc gcggccttct tttttccctt cccgatataa gaattacgcc atccgttccg
    21121 tgcggaggtg aggctatgaa atccatggat aaaatttcaa cgggcattgc ctacggcacc
    21181 tccgcaggca gtgctggcta ctggttttta cagtggcttg atcaggtcag tccgtcacag
    21241 tgggctgcga ttggtgtact ggggagtctg gttctgggct tcctgactta tctgacaaat
    21301 ctgtacttca aaatcagaga agacaagcgt aaggctgcac ggggagagta attcaatgac
    21361 tcaaaactat gaactgattg tgaaagggat ccgcaatttt gagaataaag ttacggtaac
    21421 tttagcgtta cgggacaaaa aacgctttga cggtgaaatt tttgacctgg acatctcgct
    21481 ggaccgtgtt gaaggtgccg cgctggagtt ttatgaggca gcagccagaa ggagcatcag
    21541 acaggtcttc ctggaagttg ctgccgggtt atgtgaaggg gatgagcagt cgccggaaaa
    21601 gcgccccgta attttagatg cgcagaatgt gtggataacc tacaaaggaa agctaccagg
    21661 aagaattact ggttctctga agactccacc gaaatggtaa ttttaccagc atatttttct
    21721 tccagtaatg ctaccagcca cttgaaagaa ttttgttgtt cctgggacca tttggggttg
    21781 cgcgattcaa gcaggagcga tgccagtgtt ggttgcattt gttctctggg aattgataag
    21841 gccagatatg aaaatgcaac agtgagggca tttacatcat cccgaagcct ggaaatgcag
    21901 tcgagcaact cctgtagaga aatggtgtta ttgtccataa ataatcctct tgattgtctt
    21961 taccttttcc cagcctgatt caacaggccg ggacagataa acatatccag ggttcagaaa
    22021 ccgataaatc ctgataaata tccatgaacg caaaaatcaa atacggcctg tcagctgctg
    22081 ttctggcgct gattggagca ggagcatctg ctcctcagat acttgaccag tttctggatg
    22141 aaaaagaggg taaccacact acggcatacc gcgatggttc cggcatatgg accatctgtc
    22201 gaggggccac gatggtggat ggtaaacctg ttattcctgg catgaaactg acgaaggaaa
    22261 aatgcgatca ggttaatgcc attgaacgag ataaggcgct ggcatgggtg gagcgcaaca
    22321 ttaaagtgcc actgaccgaa ccacagaaag cgggtatagc gtcattttgt ccctataaca
    22381 ttggccccgg taagtgtttc ccgtcgacgt tttataagcg gctgaatgcc gatgatcgta
    22441 agggggcatg cgaggcgatt cgctggtgga taaaagatgg tgggcgtgat tgccgcctga
    22501 caaaagggca gaagaatggc tgttatgggc aggtcgagcg gcgcgatcag gaaagcgcac
    22561 tggcgtgctg ggggctggac caatgaaaat taatccggat cttataggcg ttgtcgttat
    22621 tgctggcctt tcggtcgctc tcgtcaagag ttgctctcac gccagtaatc ttcagagcga
    22681 taacgacgtt ctgcgaagtg acaactctat gctggagcag gtaatcgcca cccaggcatt
    22741 caacttcaat cgattcaatc aggttgcaga acatgccaac agtcttaact ccctgatcga
    22801 caccagcacc gaaaaaaccg taatcgaata ccgggagatt ctccgccgtg aaaaaacctg
    22861 tgatctgcct gttcctgctg atatcgctgg tgggttgctc gaatacacgt accgtttacg
    22921 tgccagcgcc atgcacaccg ataccggcag acctaatgaa gcctatgatc gtaccgctgc
    22981 caccagctca atgacctact gccaagctgt cttgtggatt aagccgctac tggcgttgat
    23041 agaaaaagga aataataatt tttcgagtat tcgtgaaatt gatgagttgc gttacagacc
    23101 atctgagcgt ggccagtaag gttacaggaa atttaaaatc tggcgatgaa atttaatatt
    23161 agaaattcat tgcctagtga gattaataat gggcatccct ttgtgacgat aagctttaac
    23221 acattcacaa agggatattt aatatagggt ggttaaatcc cacttgctgt ggtgtttagg
    23281 ggataattca aatcttatct aactcattat aggcttcgat aataaaaatt ctgattttaa
    23341 taaggcatga ttcaatgaaa ggaattactt catagtcatt cccatggaat cttatttttt
    23401 tctcttcggt aatgactatg tttaaaggta caccattacc acttctttgt aatctcccat
    23461 tttcgaaagt aaagtcttct aatttttttc cattgatatg gttgttttta aagagaattt
    23521 ttgctgagct ccctgctgtg attaaattaa aaccatctac gctcacactt agttctttaa
    23581 cagaactctc ttctcttaag gggattggtt gtctgtgctt tacttcatta gtgagattac
    23641 acatcatttc caaccaagat tcgccagtgt gataatcctg tatagaagta aaaattttat
    23701 ataatggaga agatgatggt gggtttattt ttagcgtctt tgagaaaaat ttatcaataa
    23761 actctttttt tctgtaaggg aaatatattt tgggacgctc atcattactg cttggagaaa
    23821 tatatttatc ataggtatca ttagcgagat actctaagga acttcgtaag ttttccaaaa
    23881 tagctttgac ttttacaggc ttgatatgct gtgtctgtct cattatgtca agactttctt
    23941 caacctcttg caataagtcc aacgagcctt ctctagacat acaggtaatt ctcctttttg
    24001 aattttcaac atgttataac taattatata tttatcatat aactgtatga ataaattgtg
    24061 agattagtaa tgcaagtttc tatccatagt gttccctaag tacccgcttc catcatgttg
    24121 ttacggacgg tatggctaaa acaaagcacc tgaagttaaa cgttctgaac tttatgctcg
    24181 ttgagcatat gaattatctg taacccgatg cgctggttgt ggttcaaatt gatggtcgta
    24241 atcagcagcg gtagtgcctg atggcatgca acttcttact tataaaactt cgtatggaat
    24301 tgttgccata acgagcccag cttagcagtt tttaatgggt cctcccggca gtgtgtcctg
    24361 ccacggggcg ggagcgtcgc ggaaaaaggc tagtttttga aatttcatcc gtcatcacca
    24421 ctactgtaat agattgatat tacagtattt ttatttttgt ggtgtcgatt ttgattgttt
    24481 tttgttcatc actaacaccg tttgcctaaa gtcgttcgca agatgcatgt ttaaaacatt
    24541 ctggagcggg tatggatcga gagttaaaaa atctgacgct gaatatcagt caactggcgg
    24601 cactgtcagg tgtacatcgc cagactgctg cggcaaggct gcaaaatcta cccgttgcag
    24661 gggggcatga aagcaacctc aagctttatc gggtggttga tattgtgtcg gcatttctgg
    24721 cattaccacc gccggttgca gaaggcgaaa tggacgcgca tgagcgcaaa gcctggtatc
    24781 agtctgaacg tgagcgtctt aagttcgaac aggaaacggc gcaactcatt ccggccagtg
    24841 atgtcagacg ggagtttgcc atctgggcaa aagcggtcgt gcaggtgctg gagacattac
    24901 cggatattct ggaacgtgac tgcggtctgc agcctgccgc tgtgagccgt gttcagtcca
    24961 ttattgatga tctgcgcgat cagatagccc tgcgggtgac tgtagcaggt gcggatgatg
    25021 aggaggaatt acagcaggag gagtaatgct gaatcaggaa accgcaaagg cagcacgaac
    25081 cgattcaggt tatatccttc gcgcaccgag acgaatgcgg gttgctgatg ccgttgctca
    25141 gtatatgcgg gtgcccatgg gggcagggaa ctcagtcccg tgggatccgc tggtggcacc
    25201 gtatgttatt gagccgatga actgcctggc ctcgcgtgaa tacgacgcag tgatatttgt
    25261 tggcccggca cgaaccggca agactatcgg cctgattgac ggctgggtga tttacaacgt
    25321 gatttgcgat cctgctgata tgctgatcat tcagatgacg gaggaaaaag cccgcgaaca
    25381 ctccaaaaaa cgactcgcca gaacgtttcg cgtcagcccg gaagtggtca gtcgcctgag
    25441 tccgaacaaa aatgacaaca acgtttatga cagaacattc cttgctggca actacctgaa
    25501 aatcggctgg ccgtcagtca atatcatgtc ttcatcagat tataaatgcg tggcgctgac
    25561 ggattatgac cgttttccgg aagatattga tggtgagggg gatgccttct ctcttgcctc
    25621 aaaacgtacc accacattta tgtccagtgg tatgacgctt gtggagagtt cccccggcag
    25681 ggatgtgaag gatgtgaaat ggcgacggac ttcaccgcat gaggctccac caaccacggg
    25741 gatcctgtcg ctctataacc gtggcgatcg ccgtcgctgg tactggccct gtccacactg
    25801 tggtgagtat tttcagccct gcggcgatgt ggttgctggt ttccgtgata ttgccgatcc
    25861 tgtgctggca agtgaggcgg cttatattca gtgtccttcc tgttcaggac ggattttgcc
    25921 tgaacaaaaa cgcgagctga acggacgtgg ggtctggtta cgggatggtg aatccatcaa
    25981 tgcagatggc agtcgttatg gtgatccccg gcgttcacgt attgcgtcat tctggatgga
    26041 gggtccggca gctgcttatc agacactctc gcaactcgtt tacaaactgc ttactgcaga
    26101 acaggaatac gagacaaccg gaagtgaaga gacactcaag acggttatca ataccgactg
    26161 gggattacct tatcttcccc gcgccagcat ggagcaacga aaaagtgaat tgcttgagca
    26221 gcgggcagag ccagttcctt cccgcagtgt gccggatggc gttaatttcc ttgtggcgac
    26281 agtggatgtg caggcgggac gtcatcgccg ttttgtggtt caggtaacgg gctatggcag
    26341 ccgtggcgaa cgctggatta ttgatcgtta caacatcacg cagtcattgc gcggtgacag
    26401 cgacggggag agccagcgaa ttgatccggc cagctatccg gaagactggg atgtcctgct
    26461 gacggatgtt tttcataaaa gctggccgct ggcctccgac ccttctcaac aaatgcgact
    26521 gatggcaatg gcggtggact ccggcggtga agacggggtc actgataatg cctataaatt
    26581 ctggcgtcgt tgccgtcgtg atggccttgg taaacgtatt tacctgttta agggcgacag
    26641 catccggcgc gcaaaactga tcacccgtac attccctgat aacaccggac gaacgggccg
    26701 acgggcgcag gccgcaggtg atgtgccgct ctggcttctt cagacggatg ccctgaaaga
    26761 ccgggtgaat aacgcgttat ggcgtgactc gccaggtccc ggctatgtgc atttccctga
    26821 ctggctgggg agctggtttt acgacgaact gacgtatgaa gagcggagca gtgacgggaa
    26881 atggagtaag ccgggtcgcg gtgccaacga agcttttgac ctgatggtgt atgccgaggc
    26941 tctggtcatt ctgcatggat acgaaaagat ccgctggccg gatgcaccgg agtgggcgag
    27001 ccgggaaacc tggctggagt gtgtcccgga cagtaccgaa ccgtcaccct caccggaacc
    27061 ggtacccacg cctgttaaaa aacaaaaacg gaagaaaaca gtaactgacg atgttaaccc
    27121 ctggctgact tccggaggat ggttatgaat cagaatgata ttgaagccat gattcagcgt
    27181 tatacggaag ctgaaatggc ggtgctggac ggaaaatccg tcacttttaa tggtcagcag
    27241 atgaccatgg aaaacttatc tgagatccgg caggggcggc aggagtggga gcgccgcctt
    27301 gcggctctga ttacacgacg acgggggcat cccgggtacc ggctggcgag gttctgatgg
    27361 catttcttga tgatgtgatt ggcgtttttt caccaggatg gaaagcggca aggctgcgtt
    27421 cccgtgcggt gatccaggct tatgaggccg taaaaacgac gcggacacac aaagcccgac
    27481 gggagaaccg aactgccgac cagttaagcc agtacggggc cgtgtcgtta cgtgagcagg
    27541 ctcgttacct tgataacaac cacgatctgg tcattggtgt atttgacaag ctggaagaac
    27601 gggtggtggg gaaaaacggg attattgtcg agccacatcc ggtattacgc aatggggcca
    27661 ttgcccgtga tctggcagcg gagatacgta cccgatggag tgaatggtct gtcagtccgg
    27721 aggtcaccgg gcagtttacc cgtccgatgc tggaacgtct gatgctgcgt acctggctgc
    27781 gcgatggtga ggtgtttgcc cagatggttt ccgggcgcat aaacagcctg acgccttctg
    27841 ccggtgttca tttctggctg gaggcgctcg agccggactt tattcccatg accagtgatg
    27901 agagcaacag gctgaatcag ggcgtgtttg ttgatgactg ggggcgtccc gaaaaatatc
    27961 tggtgtataa aagccgtccc gtatccgggc ggcagatgga aaccaaagaa gtggatgcag
    28021 agcgaatgct gcatcttaaa tttgttcgcc gtctgcacca gatgcgcggg acgtctttgt
    28081 tgtccggtgt gctgatccgc ctcagtgctc tgaaagagta tgaagattct gagctgactg
    28141 cagcaaggat cgccgctgct ctggggatgt acatccggaa aggcgacggg cagagctatg
    28201 aaccggatgg taatggcagc aaggagaatg aacgcgagct taccattcag ccaggcatta
    28261 tttacgacga tctgaaaccc ggcgaagaaa tcggaatggt gaagtcggat cgtcccaatc
    28321 ctaaccttga aacttttcgt aatggtcagt tgcgtgccgt ggcggcgggc agtcgtctga
    28381 gtttttccag tacggcacgc aactataacg gcacttacag cgcccagcgt caggaactgg
    28441 ttgagtctac tgatggctac ctgatcctgc aggactggtt tattggtgcc gtcacccgcc
    28501 cgatgtatcg tgcctggctg aaacaggctg tggcatccgg tgttatcagg ctaccccgtg
    28561 atcttgaccg ttcttcactg tataccgcgg tgtattccgg accggtgatg ccgtggattg
    28621 accctgttaa ggaggctgag gcctggaaaa tccagattcg tggtggagcg gcgacagaat
    28681 cagactgggt acgtgctggt ggtcgtaatc cggatgatgt caaacgtcgg cgcaaggccg
    28741 aaattgatga aaaccgcaag ctggatctgg tatttgatac cgatccggcc agtgataaag
    28801 gaggcagcag tgccgcaacg aaacgacagg agccgcagca caccgacgac cagtccgaag
    28861 aataattcct ggttcaggat gcaggctggt caccagagtg acgcggatat ttatatttat
    28921 gacgagattg gtttctgggg tgttacagcg aagcagttta tcagtgatct gaatgcactg
    28981 ggcgatatca cccacattaa tctccatatt aattcaccgg gtggcgatgt ctttgaaggc
    29041 atcgccattt ttaatgcgct gaaaacacat ggtgcgtcca ttaccgttta tgtcgacggt
    29101 gtggcggcgt caatggcgtc ggtcattgcg atggtgggaa acccggtcat tatgccggaa
    29161 aacaccttca tgatgattca taaaccattt ggctttacgg gcggtgatgc ggaggacatg
    29221 cgcacctatg ccgacctgct cgataaggtt gaggcggttc tgttacccgc ttatgcacag
    29281 aaaaccggga aaaccaccga tgaaattgct gccatgctgg tggatgagac ctggatgtcc
    29341 ggtgccgaat gtctggcaca tggatttgct gatcaggtaa cgccagccgt taaggcaatg
    29401 gcatgtattc agtcaaaacg tacagaggaa tttaaaaaga tgccggaatc cattcgaaac
    29461 atgattactc cgccacgcaa cagtgctcca cgcgtacagg atgatgaacc tgcagcctcc
    29521 cggacgccag tgcaggcagc agcagcaccc gtggtggatg aaaacagcat ccgtgcgcag
    29581 gtcctggcag agcaaaaagc gcgtgtaaac ggtattaatg atctgtttgc catgtttggc
    29641 gggcgttatc agtcgctgca ggctcagtgt cttgccgatc ctgaatgttc gctggagcag
    29701 gcccgcgaaa agctgttgaa cgagatgggg cacgagtcca cgccatccaa taaaaatacc
    29761 ccggctcata tttatgccgg taacggtaat tttgtggggg acgggatccg ccaggcgctg
    29821 atggcgcgtg ccggatttga aaaaaccgaa cgtgataatg tctacaacgg gatgaccctg
    29881 cgtgaatatg cccgtatgtc actgactgaa cggggtattg gggtttccag ttataacccg
    29941 atgcagatgg tcggtgcggc gttcacacac agtacgtctg acttcggtaa tattctgctg
    30001 gatgttgcga acaaagccat tctgcagggc tgggaagatg cccctgaaac ctatgaacag
    30061 tggacgcgga aaggtcagtt gtctgatttt aaaattgccc atcgtgtggg tatggggggc
    30121 ttcagtgctc tgcgtcaggt gcgtgaaggg gcggaatata aatacgtcac caccggagat
    30181 aaacaggcca ctattgcact ggcgacctat ggcgagctgt tcagtatcac ccgtcaggcc
    30241 attatcaatg atgatctgaa tatgctgacc gatgtcccga tgaaactggg ccgtgcggcg
    30301 aaatccacta ttgccgatct ggtttatgcc attctgacgt ctaacccgaa aatctccaca
    30361 gataatgtaa gtctgttcga taaagcgaaa catgcaaacg tactggagag cgctgcaatg
    30421 gacgtggcat cgctggataa agcccgccag ttgatgcgcg ttcagaaaga gggggagcgt
    30481 catctgaata ttcgtcctgc gttcgtactg gtaccgacgg cgatggagtc tgttgctaac
    30541 caggtcattc gctcctcaag tgtcaagggg gctgacatta acgccggtat tattaacccg
    30601 gtgaaagatt ttgcgaccgt tattgcagag cctcgtcttg atgataacag ccagaccacc
    30661 ttctacctgg ctgcgtcaaa aggctccgat acgattgaag tggcttatct caacggtgtg
    30721 gatacgccat atattgatca gatggagggc ttcagtgtgg atggcgtgac aacgaaagtg
    30781 cgtattgacg ccggtgtcgc gccagttgat caccgcggtc tggtgaaatg tacggcgtaa
    30841 acgtcgcaga caacaactct gatggcccgt aagggctttt tttgtacctg aaatcagccc
    30901 ctgaacgggg ctgtgcggag gcagttatgg caaagaattt tgtagaagaa ggaaaaacgg
    30961 tggcgattgt tgccagtgca gccatcagca gcggagatct ggtgcaggtg ggtgatgttt
    31021 ttgcggtggc gctgaccaat attccacagg gtgaaacagg cgacggcatg accgaaggtg
    31081 tgtttatgct gcctaagctg aaaacggatg acatgaaaac gggtaagaag gtttatctga
    31141 agtccggaaa agttcagctg actaacagcg gctctgatcc gctggtcggg gttgtctggg
    31201 cagatgccgg aaccagtgca gaagaagtgc cggtaaaact caatgtctga tcccttttcc
    31261 cggctggcag cgcgtatgga tgctatcacg gtcagaaaga tgggaaagac agcctcgatt
    31321 aatgatgtcg atatgactgt gatcccggga gaaacactgg cagagctgaa tgctctgtcc
    31381 ggacctgcgg tctctctggt ggtgttttct tcgggatacc gcccacggcg cggggatcgc
    31441 gttgtttatg acggacaaca atggacggtc acacggcatg aacgttttaa cggtaagcca
    31501 atgatcttta ttgagtaaag aggtgtggga tgaaggggct tgagaatgcc atccgcaatc
    31561 tgaacagcct tgatacccat atggtgccac aggccagcgc atgggcgata aaccgtgtgg
    31621 cacagaaagc ggtctcggtt gccacccggc aggttgccgg gaataccgtt gcgggagata
    31681 accaggtgaa agggatcccc ctgaaactgg tacgtcagcg tgtccgggtg tttaaagcca
    31741 gtccgtcagg aaaaatgacg gccaggatcc gcgttaaccg gggcaatctg cccgccatta
    31801 agctggggac agcccgggtc agactggccc ggcgtggtgg aaaactgcag taccgtggca
    31861 gtgtgctgaa ggtgggtaaa tatcttttcc gggatgcgtt tattcagcaa ctggcgaatg
    31921 gtcgctggca tgtgatgcgg cgtattgatg gcaaaaatcg ttaccccatt gatgtggtga
    31981 aaatcccgct gtccggaccg ctgacacagg catttgaaga tgcccgcgac cgcatcattg
    32041 ctgcggaaat gccgaaacag ctggggtatg cactgaaaca acaactgagg ttatggctga
    32101 cccgatgaac cgacatacac aaatccgcca ggccgtactg gcacgccttc gggaacagtg
    32161 tggagacagc gccacgtttt ttgacgggct tccggcattt attgatgcgc aggaactgcc
    32221 tgccgtggcg gtgtggctga gtgatgctca gtacaccgga aaaatgacgg atgaagatga
    32281 ctggcaggct gttctgcata ttgctgtctt catccgggca caggcaccgg attcagagct
    32341 ggatatgtgg atggagagca ccattttccc ggctctgaat gatataccgg cactttccgg
    32401 actcatcgac accctgatcc ctctcggttt taactatcaa cgtgataatg agatggccac
    32461 ctgggcgatg gcggaaatca cgtaccagat cacgtacacg aattaaagga ggtggcaatg
    32521 accacaccaa atccactggc aaaaacgaaa ggtgcgggaa cgacgttctg gatgtacacc
    32581 ggcaagggcg atgcgtttgc gaacccttta tcggacactg actggctgcg tcttgcgatg
    32641 gtgaaggatc tgcaacctgg cgaaatgacc gctgatgcag aagatgacac ttatctcgat
    32701 gatgaagatg cagactggaa aacgacaacc caggggcaga aatccgtcgg tgatacttcg
    32761 gcgacgctgg cctggcgtcc gggtgacagc gggcagaaaa aactggttca gttgttcgac
    32821 tccggtgaag tctgcgcgtt tcgtatcaaa tatcccaacg gcactgttga tgttttccgt
    32881 ggctggctga gctcactggg taaaaccatt gcctcaaaag acgtgatgac ccgcacagtg
    32941 aaaatcagcg gtgtggggcg tccgcatctg gcagaggaag gcactgaaac agtgagcgtt
    33001 accgggctga cggtggcacc ggcatctgcc agtgtaaaag tgggagcaac caccacgctg
    33061 acctttacag taaaacctga cggagccagt gacaaagcga tcagtgtgca ttcgacagat
    33121 ccacagactg ccacggtgac cctgaacggg cttgtggcca cggtgaaagg cgtgaagcag
    33181 ggcagtgtca gcattgtggg catgacttct gacggcgatt ttgtggccgt ggctgcggtg
    33241 gctgtcagcg ccgcaggtta acaggacgat actcatcatt tgccccggtt atccggggct
    33301 tttttgcagg tggagaacat gatgtttctg aaacagggct cgtttaatta tgaaaagcag
    33361 tccgtggtgc tcagtgagct gtccgggctg cagagaattg aatatctggc gtttgttcag
    33421 cagcgaacgg caaagtttga tgccgaagag ggagaactgc cggaggctga acgacagatt
    33481 gcttttctgc ggatggggat ggatatcaat gcctggctgg tttcccgctc actgtggaat
    33541 gcggaacagt ctcaggatgt tgagacgctt tgcgcatccg ttattacaac atggtcgtat
    33601 gatgccctgg gagcgggggc ggagatggtt ctgtcgctga gcggtatggg agccattgag
    33661 aatgccgggg atttggagca tgaggtgctg acgccggaaa agtcctgacg cgggaaatgc
    33721 agtttgtcat gcggcttgcc cgggagttcc ggcgggcaga ctggcggcgg atgctgtcgg
    33781 aaatgtcggc cactgagctt ggtgaatggg gcgattattt ccagatgcag agcttcagtg
    33841 atgtgtggat ggatgcgcag tttgcctcgc tgaaggcatt gatcgtgaga atggtgtccg
    33901 gtagcagtga tgctgcggtg gctgatttca gccttttacc ggaagagaac gggataccgg
    33961 agcgaacgga cgaagaactg atgcatcttg gggaaggtat ttccggaggt gtgcgttatg
    34021 gaccagatag ccaacctggt cattgatttg gggattgatg cggcagagtt taaaaatgaa
    34081 attccccgta tcaaaaacct tctgaatggt gcagccagcg atgcagaacg gtcttctgcc
    34141 cgtatgcagc gttttatgga gcgtcagact caggccgccc ggcagacaat gcaggcggct
    34201 tcttcggctg caacagccgc atccgtccat gcgcagacgg tggagaagag cgcacaggct
    34261 catgaacgca tggcccgcga ggtggagcaa acccgccagc gtatggaggc actgagccag
    34321 aaaatgcgcg aggaacaggc gcaggccatg gctctggcgg aggctcagga taaagcggct
    34381 gccgcgtttt atcgtcagat tgacagtgtg aaacaggcca gtgcgggact gcaggaatta
    34441 cagcgtattc agcagcagat ccgacaggcc agaaacagtg gcgggattgg tcagcaggat
    34501 tatctggcgc tgatttctga ggttacggcg aaaaccggtg ttcttacgca ggctgaggca
    34561 gaggctaccc gacagaaagt ggcgtttatc cgtcagctta aagagcaggc aacccgccag
    34621 aatctttctt cttctgagtt gcttcgtgct aaggctgccc agctgggggt aagcagtgct
    34681 gcagaagtgt atatccgcaa aatggagcag gcaggaaaag ccacgcattc gctgggtctg
    34741 aaaagtgcag cggcccgcca ggagataggc gttctgatag gtgaactggc tcgcggcaat
    34801 ttaggtgcgc tgaggggatc cgggataacg ctggctaacc gtgccggatg gatagacaca
    34861 ctgatgtcac cgaaaggcat gatgctgggc ggggttattg gcggtattgc cgcggccgtc
    34921 tatggtctgg gtaaagcctg gtatgatggt cagaaggagg gggaagaatt taaccgccag
    34981 ttgtcgctga cggggcatta tgccggagtc actgccgggc agctgtggac gctcagtcgt
    35041 gctatttccg ggaatggtat tacgcaacat gctgcagccg gtgcgctggc tcaggtggtg
    35101 gggagtggtg catttcgtgg aaacgatatc ggtatggtgg cgagagctgc cgcacagatg
    35161 gagcgatcgg ttggccagtc ggtcagcgat accataaatc agtttaagcg gctgaaggat
    35221 gatcctgtaa atgccgcgaa ggctctggac aatgagctgc attttcttac tgccactcag
    35281 cttgagcaga tacgcgtcct tggggaacag gggcggtcca gtgatgcggc acggatagcc
    35341 atgtctgcac tggcagagga aaccggtcgg cgtactgcgg atattgataa taacctcaat
    35401 gcgctgggca gtacgctgaa gtatctgtct gatttatgga gtcgtttctg ggatgcggcc
    35461 atgaatattg gtcgtgaaga ctcgctggat gaacagattt ccgctttaca ggagaaagtg
    35521 tcgcgggcga aaagactccc ctggacggca tcatcttctc aggttgagta cgatcagcag
    35581 cgtcttaacg agcttcagga gaaaaaacgc cagaaggatt tgcaggatgc aaaagagcag
    35641 gcagagcgga attatcagga gcaacagaaa cgccgtaatg ctgaaaatgc tgcactgaac
    35701 cggatgaatg aaacggaagc agcacgacat cagcgtgaaa ttgcgcgtat taattccatg
    35761 cagtacgccg atcaggctgt cagggatgcg gcgatacaac gtgaaaatga acgttacgag
    35821 aaagccctgg catccggtaa gaaaaaaaca cgcgaccccc gtaatgatga ggccacccgg
    35881 ttattgctgc agtacagtca gcaacaggca caggtggaag gacagattgc tgctgccaga
    35941 cagtcagcag gcattgccac ggaaaggatg acagaagcgc ataaacagct tctggctctg
    36001 cagcagcgca tcagcgacct ggacgggaaa aaactgacgg cagatgaaaa gagtgtgctg
    36061 gcccgtaaag atgaactgat tcaggcactg acgctgctgg atgtaaaaca gcaggagctt
    36121 cagaaacaga cggcactcaa cgagctgaag aaaaaaacaa ttcagctgac cagtcaactg
    36181 gctgaagaag agcgcgctca gcgtcagcaa catgacctgg atatcgccac ggtgggtatg
    36241 ggtgatcagc agcggcagcg atatcaggta caactgagtc ttcgccagaa ataccagcaa
    36301 cagctggagc agttgaggcg ggatagtgag cagaaaggaa catataacac ggatgactac
    36361 agaaaggccg agcaggcgct gacggagagc ctgaaccgac aactgaatga gaatcgccgt
    36421 tactggcaac agcttgaagt tgtgcagggt aactggaaaa acggagtcct gcgtgcattt
    36481 caggatttta ccgtggatgc agataatacg gcaggaacag cagaacaggt gttctcgtca
    36541 gccttcagca acatgggaaa tggcctggca acttttgtca ctaccggcaa actcaatttc
    36601 aaatccttca cctcttctgt gctgtcagat atggcgaaaa tcctggcgca ggcaaccatg
    36661 atgaaatcga taaaagggat tggcagtgta ctgagatttg atctcagcag cctttccctg
    36721 aatgccaatg gggggattta tcagtctgct gatttgagtc gttacagtgg cacggtggtt
    36781 aaccgtccga cgttttttgc ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct
    36841 gaagccattc tgccactgcg tcgtggtgct gacggtaagc tgggggttgt ggcggatatt
    36901 ggtggttcag gtatggcgat gtttgccccg cagtacaaca tcgagatcaa taacgatggc
    36961 acgaacgggc agataggtcc ggctgccctg aaggtggttt atgacctcgg gaaaaaagcg
    37021 gcagcggact ttatgcaaca gcaggcccgt gatggtggtc ggttaagtgg agcatatcgg
    37081 taatggagac gtttcactgg aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag
    37141 tggtgacagt gaagctgggc gatggttatg aacagcgtcg tgcggcggga ctgaataacc
    37201 agttgtcgat ttacagcgtg acgatacgtg ttcgtaaatg tgaacaccca tctttaaaag
    37261 cctttctgga acggcacggt ggcgtccgcg catttcagtg gacgccacct tatgactgga
    37321 agccgatcag ggtggtttgt cgtaaatggt cggcaagcgt gggggcgctg tggataacca
    37381 taacggcaga ttttgaacag gtcgtggcat aggaggctct gatgcaggat attccacagg
    37441 aaacacatca tgagacgaca cgcctcactc agtcagccca ggtggtgctc tgggaaatcg
    37501 atctgacaga ggtcggtggt gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg
    37561 agccggttac ctggcagggg cggcagtatc aggcataccc cattcagggg acgggatttg
    37621 aactgaatgg caagggcagt gctgcccgtc cgacactgac ggtttctaac ctgcacggca
    37681 tggtcaccgg gatggcggaa gacctgcaga gtctggtcgg cggaacggtg gtccggcgta
    37741 aggtttacgc ccgttttctg gatgcggtga acttcgtcaa cggaaacagt gacgccgatc
    37801 cggagcagga ggtgattagc cgctggcgca tcgagcagtg cagcgaactg agcgcggtga
    37861 gtgcctcttt tgtactgtcc acgccgacgg aaacggacgg tgccgttttt ccgggacgta
    37921 tcatgctggc caacacctgc acctggacct
//
LOCUS       DAEVZA010000033        37760 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEVZA010000033 DAEVZA010000000
VERSION     DAEVZA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.033"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..691)
                     /gene="aes"
                     /locus_tag="LAF92_001748"
     CDS             complement(<1..691)
                     /gene="aes"
                     /locus_tag="LAF92_001748"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBI9790768.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLS"
     gene            843..2147
                     /gene="gsk"
                     /locus_tag="LAF92_001749"
     CDS             843..2147
                     /gene="gsk"
                     /locus_tag="LAF92_001749"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBI9790769.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(2280..3956)
                     /locus_tag="LAF92_001750"
     CDS             complement(2280..3956)
                     /locus_tag="LAF92_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBI9790770.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(4194..5414)
                     /gene="fsr"
                     /locus_tag="LAF92_001751"
     CDS             complement(4194..5414)
                     /gene="fsr"
                     /locus_tag="LAF92_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBI9790771.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            5632..7284
                     /gene="ushA"
                     /locus_tag="LAF92_001752"
     CDS             5632..7284
                     /gene="ushA"
                     /locus_tag="LAF92_001752"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBI9790772.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(7321..7800)
                     /gene="ybaK"
                     /locus_tag="LAF92_001753"
     CDS             complement(7321..7800)
                     /gene="ybaK"
                     /locus_tag="LAF92_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBI9790773.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(8004..8798)
                     /locus_tag="LAF92_001754"
     CDS             complement(8004..8798)
                     /locus_tag="LAF92_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBI9790774.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            8936..9277
                     /locus_tag="LAF92_001755"
     CDS             8936..9277
                     /locus_tag="LAF92_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBI9790775.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(9391..11895)
                     /gene="copA"
                     /locus_tag="LAF92_001756"
     CDS             complement(9391..11895)
                     /gene="copA"
                     /locus_tag="LAF92_001756"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBI9790776.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            12157..13089
                     /gene="glsA"
                     /locus_tag="LAF92_001757"
     CDS             12157..13089
                     /gene="glsA"
                     /locus_tag="LAF92_001757"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBI9790777.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            13092..14384
                     /locus_tag="LAF92_001758"
     CDS             13092..14384
                     /locus_tag="LAF92_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI9790778.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     LGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            14509..14916
                     /gene="cueR"
                     /locus_tag="LAF92_001759"
     CDS             14509..14916
                     /gene="cueR"
                     /locus_tag="LAF92_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBI9790779.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(14917..15375)
                     /locus_tag="LAF92_001760"
     CDS             complement(14917..15375)
                     /locus_tag="LAF92_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBI9790780.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(15372..16289)
                     /locus_tag="LAF92_001761"
     CDS             complement(15372..16289)
                     /locus_tag="LAF92_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBI9790781.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            16435..17112
                     /gene="fetA"
                     /locus_tag="LAF92_001762"
     CDS             16435..17112
                     /gene="fetA"
                     /locus_tag="LAF92_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBI9790782.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            17099..17878
                     /gene="fetB"
                     /locus_tag="LAF92_001763"
     CDS             17099..17878
                     /gene="fetB"
                     /locus_tag="LAF92_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBI9790783.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(17941..18795)
                     /gene="cnoX"
                     /locus_tag="LAF92_001764"
     CDS             complement(17941..18795)
                     /gene="cnoX"
                     /locus_tag="LAF92_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBI9790784.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(18856..19665)
                     /gene="ybbO"
                     /locus_tag="LAF92_001765"
     CDS             complement(18856..19665)
                     /gene="ybbO"
                     /locus_tag="LAF92_001765"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBI9790785.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(19655..20278)
                     /gene="tesA"
                     /locus_tag="LAF92_001766"
     CDS             complement(19655..20278)
                     /gene="tesA"
                     /locus_tag="LAF92_001766"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBI9790786.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            20249..20935
                     /locus_tag="LAF92_001767"
     CDS             20249..20935
                     /locus_tag="LAF92_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI9790787.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            20932..23346
                     /locus_tag="LAF92_001768"
     CDS             20932..23346
                     /locus_tag="LAF92_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9790788.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLKVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(24260..25354)
                     /gene="mnmH"
                     /locus_tag="LAF92_001769"
     CDS             complement(24260..25354)
                     /gene="mnmH"
                     /locus_tag="LAF92_001769"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBI9790789.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQAIIELSQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELSARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(25423..26349)
                     /gene="allS"
                     /locus_tag="LAF92_001770"
     CDS             complement(25423..26349)
                     /gene="allS"
                     /locus_tag="LAF92_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBI9790790.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26579..27061
                     /gene="allA"
                     /locus_tag="LAF92_001771"
     CDS             26579..27061
                     /gene="allA"
                     /locus_tag="LAF92_001771"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBI9790791.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            27139..27954
                     /gene="allR"
                     /locus_tag="LAF92_001772"
     CDS             27139..27954
                     /gene="allR"
                     /locus_tag="LAF92_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBI9790792.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            28044..29825
                     /gene="gcl"
                     /locus_tag="LAF92_001773"
     CDS             28044..29825
                     /gene="gcl"
                     /locus_tag="LAF92_001773"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBI9790793.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAQRPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            29838..30614
                     /gene="hyi"
                     /locus_tag="LAF92_001774"
     CDS             29838..30614
                     /gene="hyi"
                     /locus_tag="LAF92_001774"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBI9790794.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            30714..31592
                     /gene="glxR"
                     /locus_tag="LAF92_001775"
     CDS             30714..31592
                     /gene="glxR"
                     /locus_tag="LAF92_001775"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBI9790795.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            31762..33216
                     /locus_tag="LAF92_001776"
     CDS             31762..33216
                     /locus_tag="LAF92_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HBI9790796.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLIENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTVEKTGEQKTIG"
     gene            33276..34637
                     /gene="allB"
                     /locus_tag="LAF92_001777"
     CDS             33276..34637
                     /gene="allB"
                     /locus_tag="LAF92_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HBI9790797.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            34694..35995
                     /locus_tag="LAF92_001778"
     CDS             34694..35995
                     /locus_tag="LAF92_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBI9790798.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTVLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            36017..37162
                     /gene="glxK"
                     /locus_tag="LAF92_001779"
     CDS             36017..37162
                     /gene="glxK"
                     /locus_tag="LAF92_001779"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HBI9790799.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(37291..>37760)
                     /locus_tag="LAF92_001780"
     CDS             complement(37291..>37760)
                     /locus_tag="LAF92_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBI9790800.1"
                     /translation="GYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGN
                     ASELERIHYEGMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILS
                     GQGVYNLDNNWIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
BASE COUNT         8910 a         9575 c        10068 g         9207 t
ORIGIN      
        1 tgcttaaata cgcctcttca tacatctgca aatcctgttg cgttaagcca tcccagacac
       61 cgcccaacag acgacgagtc acggaatccc gtaatccgta aagcccatac cacagcaaaa
      121 cacccgcaat tttaccgcaa tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg
      181 ccagcatggc acctgcggaa tcaccggcaa agccaatgcg ggacatattg atttgataat
      241 cctccgcctg ctggtggaaa taacaacaag cagccacaat ttcctctatc gcttgcggaa
      301 aacgcgcttc aggtgaaagg gggtaatcaa taccaatcac cgtacattgg ctgtagcttg
      361 ccagcaggcg catgatgcga tcgtgggtat cgagattgcc gagaataaaa ccgcctccat
      421 gcaaataaaa tagcgtcgcc gggctatctg gctgcggaca aaagagacgt gtttccacct
      481 gcccatattt tgttggaacc atgtaagctc tggttgccat ttctggagcg cccgcattcc
      541 agaatcggcg ctcaagcgtg taatactgtc gttgctcagc aatcgttccc gttgcgggcc
      601 aggacggtaa atccggctga agagtattca caacggtctt catttcagca gaaataaggt
      661 ccagaacagg tagtttgttt tccggcttca tacaaaaact cctttcaaat tacgtcattg
      721 taaggaacca ctgccatgaa aatgcgatcc cgcctgctga tattgaaact ggctgcgtct
      781 cgcgcgctcc cgtcagattg tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa
      841 ccatgaaatt tcccggtaaa cgtaaatcca aacattactt ccctgtaaat gcacgcgatc
      901 cgctgcttca gcaattccag ccagaaaacg aaaccagcgc cgcctgggtc gtaggtattg
      961 accagacgct ggtcgatatt gaagcgaaag tggatgatga attcattgag cgttatggat
     1021 taagcgccgg gcattcactg gtgattgagg atgatgtagc cgaagcgctt tatcaggaac
     1081 taaaacagaa aaacctgatt acccatcagt ttgcgggtgg caccattggt aacaccatgc
     1141 ataactactc ggtgctcgcg gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata
     1201 ttgaaattgg cagctatgcc tatcgttacc tgtgtaacac ctccagccgt accgatctta
     1261 actatctaca aggcgtggat ggcccgattg gtcgttgctt tacgctgatt ggcgagtccg
     1321 gggaacgtac ctttgctatc agccctggcc acatgaacca gctgcgggct gaaagtattc
     1381 cggaagatgt gattgccgga gcctcggctc tggttctcac ctcttatctg gtgcgttgca
     1441 agccgggtga acccatgccg gaagcaacca tgaaagccat tgagtacgcg aagaaatata
     1501 acgtaccggt ggtgctgacg ctgggaacta agtttgtcat tgccgagaat ccgcagtggt
     1561 ggcagcaatt cctcaaagac cacgtctcta tccttgcgat gaacgaagat gaagccgaag
     1621 cgttgaccgg agaaagcgat ccgttgttgg catctgacaa ggcgctggac tgggtagatc
     1681 tggtgctgtg caccgccggg ccaatcggct tgtatatggc gggctttacc gaagacgaag
     1741 cgaaacgtaa aacccagcat ccgctgctgc cgggcgctat agcggaattc aaccagtatg
     1801 agtttagccg cgccatgcgc cacaaggatt gccagaatcc gctgcgtgta tattcgcaca
     1861 ttgcgccgta catgggcggg ccggaaaaaa tcatgaacac taatggagcg ggggatggcg
     1921 cattggcagc gttgctgcat gacattaccg ccaacagcta ccatcgtagc aacgtaccaa
     1981 actccagcaa acataaattc acctggttaa cttattcatc gttagcgcag gtgtgtaaat
     2041 atgctaaccg tgtgagctat caggtactga accagcattc acctcgttta acgcgcggct
     2101 tgccggagcg tgaagacagc ctggaagagt cttactggga tcgttaagtt atcgtcggtt
     2161 cgtaggccag ataaggcgtt cacgccgcat ctggcatttg gctctcgatg cctgatgcga
     2221 cgctggcgcg tcttatcatg cctacatatt tttcatattt tacatccggc aaccaccgtt
     2281 taccccgtca ccacctcacc cgccggtggc gtttccagca gttccagcat ggtacgggcg
     2341 atttcacgct cgcccatcac tacctgattc gcaccacgtt cggtgatata cgccacttca
     2401 tcgtcataat gggcgcgggc aataatctca atatccggat ttttcgcgcg ggcagatgcc
     2461 acaatctcac ccgcttcata accgttggga atcgtcagga tcagccattt tgcacattcc
     2521 agatgcgcca gttgcataat ttcttcgttc gccgcattgc ccaatactgc gcggaccccg
     2581 cgctctcgca gctcatcaac acgggttcgt gacgtctcaa tcaccaccag cggaatatca
     2641 gaggcgagca atttctcccc cagcaggctg cctacacgac cgtaacccac cagtagcgca
     2701 tggttgcaaa tatccactgg gatctgcttc tcttcttcga ttgcctcttc cagcgtctgc
     2761 tcttccagcg tttcggtctt cgccagatat ttctccagta gtgcgaacag taccgggttg
     2821 agcataatcg acaggatcgc ccctgccagt accaggtttt gtccggcctg cggcagtaaa
     2881 ttcaatgcca ttcccagtcc cgccaggata aacgcgaact caccaatctg cgccaggctg
     2941 gcggcgatgg ttaatgccgt acgttgggag tgaccaaaca gtcgcaccag gaaaaatgcg
     3001 gctaacgact taccaaacag aataatcgcc agcgtcgcca gcactgccag cggttgctga
     3061 atcagaatta acggatcaaa caacatcccg acggagacaa aaaacagcac cgcaaacgcg
     3121 tcgcgcaatg gcagcgtatc gtgggcggca cggtgactca gttcagactc gttcagtacc
     3181 atcccggcaa agaacgcacc gagtgcaaag gagacatcaa acagctctac cgcaccaaag
     3241 gcaatcccta acgccagcgc cagcaccgac agggtaaaca gctcgcgaga accggttgcc
     3301 gcgctgcgtg ccataatcca cggcaccaga cggcgaccta ccagcatcat aatggcgata
     3361 aatgcgatca ctttgccgat ggtgatcccc atatcgactg caagagtggc aaagcccaca
     3421 tcgccctgtt ccatcattcc tgccactgcg ggcagcaaca ccagcgtcag aaccattacc
     3481 aggtcttcca caatcaacca accgatggcg atttgcccac gctgactgtc aattaattgc
     3541 cgttcttcaa gtgcgcgcag taacaccacg gtactggcgg tggaaagaca taaaccgaac
     3601 acgataccgg tcattaacga ccagcccagc acggcggaga gcgccatacc cagcagcgtc
     3661 gccacggcta tctgggcgat cgcgccggga atggcgatgg cctttaccgc catcaaatcc
     3721 ttcagcgaaa agtgcaaacc gacgccaaac atcaacagaa tgacgcccag ttcagccagt
     3781 tccggcgcaa gcttggtatc ggcaacaaag cccggagtga atggtcctgc cagcacaccc
     3841 gctaacagat atcccaccag aggagaaata cgtagtttat tggccagcat gccgaggata
     3901 aaggcgagca caaggccgcc aacaatggtg gtgataagcg gggtggcgtg atgcattccg
     3961 tctccttttc ctggtggtta ttgtccattt ttggccggga aaaccaaaat tacaggtaat
     4021 agtttatgac aatttcattg atgatgttca tgaataatta ttgaattttg cagaaaaatg
     4081 gaattagctg caaaaaaagc acggatcgga aaacagaagg cggtttaaca gaggaaaggc
     4141 ttatggcgac tgtaaaacga tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct
     4201 ttatgccggt tatcaggcag gaatatggtc aacatcccca atagtggcag gaaagcacag
     4261 attttataga ctaactcgat gctggtgtga tcggcgataa gccccagaac tgccgctccc
     4321 agacctccca tgccaaaagc aaaaccgaaa aagagtccag aaaccatacc gatacgtccc
     4381 ggaagtagct cctgagcgta gaccagaatg gcagagaatg ccgaagcgag gataaatcca
     4441 ataatcaccg ttaaaacccc cgtccagtgc aggctggcgt agggtaaaat cagcgtaaac
     4501 ggcgcaacgc cgaggataga gccccaaatc acatatttcc gtccaatttt atcccctaca
     4561 ggcccgccga tcaccgtacc tgccgcaacg gcaaacagga aggcaaacag atgaagctgg
     4621 gcattctgga tagataatcc gaatttttgc atcagataaa aggtgtaata gctgctgatg
     4681 ctcgccatat agaaatattt cgagaaaatg aggattaaca gaatgctgac tgccagtacc
     4741 actttgttgc gcggcagtgg attgattatc gtcgctttgg attttccttt attcattcgg
     4801 tgctgtgccg agtaccaacg gctaatttgc gccaacacca cgatcgccag cagtgccgca
     4861 agcacaaacc aggcaacgtt gcctttgcca taaggcgcga taatcaccgc cgccagcaag
     4921 ggtcccaggg aactgccaaa gttgccgccg acctgaaaga tagattgcgc caggccatgc
     4981 cgcccgccgg aagccatacg ggctacgcga gaagattccg gatgaaagac cgatgaaccg
     5041 gtaccgacca gcgccgccgc cagcagaact gcgccaaaac tgcccgccag cgcaagcagc
     5101 accagaccac ttaaggtaaa gcacatgcca attggcaacg accacggcat cggatattta
     5161 tcggtccagt agccgaccac tggttgcagt agcgaagagg cgagctggaa ggtgagggtt
     5221 atcatgccaa tctgcataaa tgtcagagaa aattctgact gaagcagcgg ataaatcgcc
     5281 agaatcagcg attggatcat gtcgttcagc agatgtgaga ggctgatagc acctaaaata
     5341 ccaaacgatg ttcgggcctt ggtcgttgac gcagccgcgc ccgccacagg ctggggttgt
     5401 tcactcattg ccataggaaa gtcacttttt cagggttgcg atgtaaagaa tgatcttatt
     5461 tgtgattatt acccgactaa catacctgta tgcgtcgcct gaaggaagtc tcaacgccga
     5521 atacagaatt tctaatctgg atgcagattt atcttcaccg gacgcagact tgtctatgat
     5581 gtcgcgtcat actatttttc aacacgttga aatcaggtca gggagagaag tatgaaatta
     5641 ttgcagcggg gcgtggcgtt agcgctgtta accacattta cactggcgag tgaaactgct
     5701 ctggcgtatg agcaggataa aacctacaaa attacagttc tgcataccaa tgatcatcat
     5761 gggcattttt ggcgcaatga atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg
     5821 gatggtatcc gcaaagaggt tgcggctgaa ggcggtagcg tgctgctact ttccggtggc
     5881 gacattaaca ctggcgtgcc cgagtctgac ttacaggatg ccgaacctga ttttcgcggt
     5941 atgaatctgg tgggctatga cgcgatggcg atcggtaatc atgaatttga taatccgctc
     6001 accgtattac gccagcagga aaagtgggcc aagttcccgt tgctttccgc gaatatctac
     6061 cagaaaagta ctggcgagcg cctgtttaaa ccgtgggcgc tgtttaagcg tcaggatctg
     6121 aaaattgccg tcattgggct gacaaccgat gacacagcaa aaattggtaa cccggaatac
     6181 ttcactgatg tcgaatttcg taagcccgcc gatgaagcga agctggtgat tcaggagctg
     6241 caacagacag aaaagccaga cattattatc gcggcgaccc atatggggca ttacgataat
     6301 ggtgagcacg gctctaacgc accgggcgat gtggagatgg cacgcgcgct gcctgccgga
     6361 tcgctggcga tgatcgtcgg tggtcactcg caagatccgg tctgcatggc ggcagaaaac
     6421 aaaaaacagg tcgattacgt gccgggtacg ccatgcaagc cagatcaaca aaacggcatc
     6481 tggattgtgc aggcgcatga gtggggcaaa tacgtgggac gggctgattt tgagtttcgt
     6541 aatggcgaaa tgaaaatggt taactaccag ctgattccgg tgaacctgaa gaagaaagtg
     6601 acctgggaag acgggaaaag cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag
     6661 caaatgatct cgctgttatc accgttccag aacaaaggca aagcgcagct ggaagtgaaa
     6721 ataggcgaaa ccaatggtcg tctggaaggc gatcgtgaca aagtgcgttt tgtacagact
     6781 aatatggggc ggttgattct ggcagcccaa atggatcgca ctggtgccga ctttgcggtg
     6841 atgagcggag gcggaattcg tgattctatc gaagcaggcg atatcagcta taaaaacgtg
     6901 ctgaaagtgc agccattcgg caatgtggtg gtgtatgccg acatgaccgg taaagaggtg
     6961 attgattacc tgaccgccgt cgcgcagatg aagccagatt caggtgccta cccgcaattt
     7021 gccaacgtta gctttgtggc gaaagacggc aaactgaacg accttaaaat caaaggcgaa
     7081 ccggtcgatc cggcgaaaac ttaccgtatg gcgacattaa acttcaatgc caccggcggt
     7141 gatggatatc cgcgccttga taacaaaccg ggctatgtga ataccggctt tattgatgcc
     7201 gaagtgctga aagcgtatat ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg
     7261 aaaggtgagg tgagctggca gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc
     7321 ttaatcgcgg cgggcgatat cagcaaattt ggcatcgagg atctttgcca gatcgcctgc
     7381 cgccagttcg atatccagtc cgcgcttgcc gccggaaaca taaatagtgg caaattcttg
     7441 tgcaggggcg tcgataattg ttggcagacg ttttttctgc cccagtgggc taatcccccc
     7501 taccagatac cccgtcgaac gctgcgcggc catcggatcg gccatctcaa ctttcttggc
     7561 acccagcgct tttgctactt ttttaagatc cagttgacct gcgaccggcg taacggccac
     7621 ggcaaggtgt ttcatatcac cgttcactgc caccagcagc gttttgtaga cctgatccgg
     7681 attcaaacct aattttttga cgacttcatc gccaaaattg gtttcagccg gatcgtgctc
     7741 gtaggtatgg atttgaaacg aaatcttgtt tttttcgagt aatttaactg cgggtgtcat
     7801 ggtaattttc ctgctttaaa caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa
     7861 aaaaagagtc atcttgccta agagtattgg caggatggtg agattgagcg acaatcgagt
     7921 tacaccgtcg cttaaagtga cggcataata ataaaaaaat gaaattcctc tttgacgggc
     7981 caatagcgat attggccatt tttttagcgc aacatttgcg gtaaattccc ttctccatac
     8041 aggtgtagtg caccgaccgc gaccacatat cgccccggcg gcatggcgcg taatttatcc
     8101 cgccaggcga gatttcgctg atgcatcagc acatcgtaca gcgactgact gaacgtattg
     8161 ggcagcgtta tatcattatt ttgcggcggt gcattcagcc accagctcat catttgttgc
     8221 agcaaccgtg cgttggtatg ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg
     8281 tcagggagct ggagcaacat ggcaatctgg ttttcagccc cttccagttc aatcacgggt
     8341 ttatgttgtt gcttcgccgc ctgcaataac tggtaatcga taccgtattc tgcccgcagc
     8401 cccagttttt gtgcctgcgt cgcctgaaga accatcgcga tttgccacag cggttgagta
     8461 gaaaagagcg acggagaaat gcccatctcc tggctaatgt gctgcagatt ttggagttgc
     8521 tcctcgttaa tgcgctcttc cagcgcctcg caagtaggca aattagcaaa aggcgtatcg
     8581 ctggtggaaa catccgcctc gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa
     8641 cgggtgggca ggggagccat atcgtggcta cccatatgaa tactgccaat cagatgaaaa
     8701 tggcgattgc cggggagggt gatatcgatg gcaggccagg agtaatgatt accgcgcagc
     8761 gcggcccaaa gcgtttttac ccggtacaac agatccatac gacctccctt tgtgaaatat
     8821 catgctagcg cgcggggagg gatggcgcaa atggagttgg ggacttgttt aatgtgtttg
     8881 tatgattcag tatgttcttg catcgctatt cacagggaag caacagttaa aaaccatgaa
     8941 acaggcaaca agaaaaccga cgacacccgg agatattctt ctctatgaat atctggaacc
     9001 gctcgatttg aaaatcaatg agttagcaga gttgctgcat gttcatcgta atagcgtcag
     9061 tgcactgatc aataacaatc gtaaactcac cactgagatg gcattccgtc tggcgaaagt
     9121 ttttgatacc acagtcgatt ttaggctaaa cctccaggcg gcagttgatc tttgggaagt
     9181 tgaaaacaac atgcgcaccc aggaagaatt gggacggatt gaaacagtgg ctgaatattt
     9241 ggcacgccgt gaagagcgtg caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg
     9301 taggccggat aaggcgttta cgccgcatcc ggcagtcatg cgtcgatgcc aaatgcgccg
     9361 ccctaaagca gcgcatccgc aatgacgtac ttattccttc ggtttaaacc gcagcaaccg
     9421 gttggcgtta ctcactacgg taatcgacga gagcgccatt gccgctccgg caactaccgg
     9481 gttaagcagt gttccagtaa acggccacaa aataccggcg gcgaccggaa taccgatgct
     9541 gttgtagata aaggcaccga gcaggttctg cttcatgttg cgcagcgttg cgcgggaaat
     9601 cgcgagcgca tccgcaacgc ccatcaggct atggcgcatc agggtaatcg ccgcggtttc
     9661 aatggcaaca tcactgccgc cacccatcgc aatgccgaca tccgcctggg ccagcgctgg
     9721 cgcgtcgtta atgccgtcgc ccaccattgc cacctgacgt ccttcacttt gcagacgttt
     9781 gatcgcttcg gctttaccgt ccggcagcac tccggcaatc acttcatcaa ttccagcttc
     9841 tttggcgatc gcattggcgg tggttgggtt atccccggtc aacatcacca gacgatatcc
     9901 cgctttatgc aggcgttgca gcgccgccac gctatcacta cgcaacggat cgcgtacagc
     9961 cagcagggct actgctttcc cgtcaatcgc cagcagcaca ggcgttgctc cttgcgatgc
    10021 ctgagcagta atctccgctt cgatagcttt ggtaccaacc tgttgctcat ttaacagcgc
    10081 ctgattgccc agcaataacg catgaccttc agcttcaccg ctcacgccca gcccgcgcaa
    10141 tgtgcggaaa ccgttgacct gcggcagctg catatcacct gctttatcga ggatcgcccg
    10201 tgccagcgga tggctggaac cttgctccag tgccgccgcc agacgcaatg cctgcgcttc
    10261 atcaacatca gcaaacgttt tcactgcgac aacctgcggc ttcccttcgg tcagcgtccc
    10321 ggttttatcg aacacgacgg tatcgagtgt actggcgcgt tgcagcgcgt cggcgtcccg
    10381 caccagcacg ccaaactcag ccgcccgccc gacgccggaa ataatcgaca tcggcgtcgc
    10441 cagccccagc gcacacggac aggcgataat cagtaccgtg gtggcaatca ccagggtata
    10501 aacaatttgc ggtgccggac caaagaagta ccagattgcc gcactgacca gcgcaatcac
    10561 caccaccaca ggtacaaata cggctgagat tttatccgcc agctgaccga tttctggctt
    10621 gctgctctgg gcctggcgca ccatgcgaat gatccgtgac agcgtggtat ggctgccaac
    10681 cgcactggca cgaaacagca cgctaccgtc ctgtaccact gtcccggcat ggacgctttc
    10741 gccttcgcct ttttgctgcg ggattggttc gcccgtcagc atcgcttcat ccagccatgc
    10801 ttcgccctgg gtaatctcgc catctaccgg cacgcgatcg ccggtcgtca ggcgcagcaa
    10861 catacctggc tgcacttccg ccagaggcac gcttttttca ccttcgtcag taaccaggcg
    10921 cgccgtcggt ggggttaaat cgagtaattt ctccagcgcc ttagaagaac gctgacgtgc
    10981 gcgcgcttcc agcatatggc cgagattaat cagaccgata atcatcgcac tggcttcata
    11041 gtaaagatgc cgcgcttcca tcgggaacca ctgcggccac aggttgacgc tcatcgaata
    11101 gagccacgcc acacccgttc ccagcgccac cagcgtatcc atcgtcgctg caccgttcag
    11161 caggcttttc catgcactgc ggtaaaaatg gccgccggcg aaaaccatca ctgccagggt
    11221 tatcaggccg ataactaacc acaagctgcg gttgtcagcg gtgaccatca tgttatcgcc
    11281 gatcatcccc cagaccatta ccgggatacc cacagccagt gcaacaattg cctgccagcg
    11341 gaagcgcttc atcgtagcta cggcggtttc ttgctggcgc tcgcggcgtt tagcgtcatc
    11401 ttcaatcgct tccgcaccgt agcccgcttt ttccaccgcc tgcactaaat cttgtgggga
    11461 ggcactgccc atcaccagcg cagtacgctc cgccaggttt acccgtgcct gagtgacgcc
    11521 cggtacgctt tgcagcgcat tttgtacgcg ggtgacacag ctggcgcagc tcatgccgct
    11581 cagcagcaac tgctggctgt catcgtcatc ggcggtcgct gccggaagcg cctcagaaac
    11641 cgctgtcagt gcttccgacg ggattgatga ctccgccagc ggtttagcct ttgggtggct
    11701 tacagatgcg tcataacccg cttgtttgat ggtttcgatc agctgttctg cactggcagt
    11761 cccggtaacg tgcgcttcag tgatagacac atccgcttgt tccacatccg ggcgctgttc
    11821 gagactttct ttcacgcgtt taacgcagtg accgcaggac aggccgtcca gggtcaggtc
    11881 gataatttgt gacataaaac actcctttaa gacagttttg actggctgtg ataaaggtta
    11941 aaccttccag caaggggaag gtcaagaaat taataaacca ggcgagtaaa agtccgtaaa
    12001 gattaaaaaa ttggctcaat ttgcatcagg attagacatt tatctctttg ttttcctgaa
    12061 gttaagttgc gggtgctaag ttaaatcaag atgcctgaaa atcggcaccg gggtgaggaa
    12121 ttacctcccg catctataaa aaggagttaa caaaagatgt tagatgcaaa caaattacag
    12181 caggcagtgg atcaggctta cacccaattt cactcactta acggcggaca aaatgccgac
    12241 tacattccct ttctggcgaa tgtacctagt caactggcag cagtggctat tgtgacctgc
    12301 gatggcaaca tctatcgcgc gggtgatagt gattaccgct ttgcgctgga atccatctcg
    12361 aaagtctgta cgttagccct tgcgttagag gatgtcggcc cgcaggcggt acaggacaaa
    12421 attggcgctg acccgaccgg attgcccttt aactcagtta tcgccttaga gttgcatggc
    12481 ggcaaaccgc tttcgccact ggtaaatgct ggcgccattg ccaccaccag cctgattaac
    12541 gctgaaaatg ttgaacaacg ctggcagcga attttacata tccaacagca actggctggt
    12601 gagcaggtag cgctctctga cgaagtcaac cagtcggaac aaacaaccaa cttccataac
    12661 cgggccattg cctggctgct gtactccgcc ggatatctct attgtgatgc aatggaagcc
    12721 tgtgacgtgt acacccgtca gtgctccacg ctcctcaata ctgttgaact ggcaacactt
    12781 ggcgcgacgc tggcggcggg gggagtgaat ccgctgacgc ataaacgcgt tcttcaggcc
    12841 gataacgtgc cgtacattct ggccgaaatg atgatggaag ggctgtatgg tcgctccggt
    12901 gactgggcat atcgcgttgg tttaccgggc aaaagcggtg taggtggcgg tattctggcg
    12961 gtcgtccctg gtgtgatggg aattgccgcg ttctcaccac cgctggacga agaaggcaac
    13021 agtgttcgcg gtcaaaaaat ggtggcatcg gtcgctaagc aactcggcta taacgtgttt
    13081 aagggctgat catgatgaac acggaaggta ataacggtaa caaacccctc ggtctatgga
    13141 acgtcgtttc catcggcatt ggggcaatgg tgggggcggg gatcttcgcg ctgctggggc
    13201 aggctgcgtt gctgatggaa gcctcgacct gggtcgcctt tgcttttggc ggtattgtgg
    13261 cgatgttttc cggttatgcc tatgcgcgtc tgggggcgag ctatcccagc aatggcggca
    13321 ttatcgactt ctttcgtcgc ggattaggca acggcgtctt ttcgctggcg ctctcgttac
    13381 tgtacctgtt gacgctggcg gtgagcatcg ccatggtcgc ccgtgctttt ggcgcttatg
    13441 ccgtgcagtt tttgcatgaa ggtagccagg aggagcacct tattttgctc tacgcgttgg
    13501 ggatcattgc ggtgatgacg cttttcaact ccttaagcaa ccatgcggta gggcggctgg
    13561 aagtgatcct cgtcggcatt aaaatgatga tcctgttatt gctgattatt gccggtgtct
    13621 ggtcgctgca accggcgcat atttccgtct ctgcgccccc cagctccggt gcgttcttct
    13681 cctgtatcgg gataaccttc cttgcctatg cgggctttgg catgatggcg aacgcggcgg
    13741 ataaagtgaa agatccgcag gtcattatgc cgcgggcgtt tctggtggcg attggcgtta
    13801 ccacgttgct ttatatctcg ctggcactgg ttttgcttag cgatgtatcg gcattagagt
    13861 tagaaaaata tgccgatacc gccgtagcgc aggctgcttc tccgctgctc ggacatgtgg
    13921 gttatgtgat cgtcgtcatc ggcgctttac tggcgacggc ttcagccatt aacgcgaacc
    13981 tgttcgccgt gtttaacatc atggacaaca tgggcagcga acgcgaactg ccgaagctaa
    14041 tgaataaatc cctgtggcgg cagagtacct ggggcaacat cattgtcgtg gtgttgatta
    14101 tgctgatgac ggcggcgcta aatttaggct cactcgccag tgttgccagc gccacctttt
    14161 tgatttgcta cctggcggtg tttgtggtgg cgatccgcct gcgtcatgat attcacgcct
    14221 cgttgccgat tcttatcgtt ggtacgttgg tgatgttgtt ggtgatcctt ggctttatct
    14281 acagtctgtg gtcccagggt agccgtgcgt tgatatggat tattggctca ctcttactca
    14341 gccttattgt ggcaatggtc atgaagcgca ataaaaccgt ataacatctc tctgtgcgca
    14401 gtacttcctg tattattgtg gtggcggtcg atattcgcac tggcaaaaaa acgtgcttga
    14461 atatctgttg aaacccttta acaaagcaca ggaggcgttg cgcgaacgat gaacatcagc
    14521 gatgtagcaa aaattaccgg cctgaccagc aaagccattc gcttctatga agagaagggg
    14581 ctggtgacgc cgccgatgcg cagcgaaaac ggttatcgca cctacacgca gcagcatctc
    14641 aacgaactga ccttactgcg ccaggcacgg caggtgggct ttaacctgga agagagcggc
    14701 gagctggtga atctgtttaa cgacccgcag cggcacagcg ccgacgtcaa acggcgcacg
    14761 ctggagaagg tggcggagat cgaacgacac attgaggagc tgcaatccat gcgcgaccag
    14821 ctgctggcac tggcgaatgc ctgccctggc gatgacagcg ccgactgccc gattatcgaa
    14881 aatctctccg gctgctgtca tcatcgggca gggtgattaa gacgagacgg ctcggatatg
    14941 tagggttatc ccttctatcg caatgacttc aacatgcgta cctgcaccga gatcctcgct
    15001 ggcgctgaca ggccatgaac tgtcaccgac gcgcatatga ccgcgcccgt tgaccagcgg
    15061 agattccagc acaaaacgtc ggccaatcag ctgctgcccg cgctggttta aatgactatc
    15121 gctgtgcttt tgttcgcgca cccgccgcga caaccatttc caccacagcc aggcggcgag
    15181 cagcgtcagg atggcaaaca ttaccccttg ccactcccaa cccagcggca ccagccagac
    15241 caccaggccg gtaatcaccg ctgccacgcc gctccacaac aaataaccat ttccgcccag
    15301 catctcggct gccagcagca aaccgccgag actgagccag aaaatatgtg gatgaacgac
    15361 cattaactcc atcatggctg agtccgtttg ttggcgctgt ctttcaccag ctcggcaatc
    15421 ccggcaatcg accccatcag gctgctggcc tctaatggca tcatcactac tttgctgtta
    15481 ctggaggaac cgatctgctg taacgcttcg gtgtatttct gcgctacgaa gtagttcacc
    15541 gcctgaatat caccggaggc gatagcttca gacaccattt tggtggcgcg ggcttctgct
    15601 tcggcggaac gttcacgcgc ttcagcctgt aaaaacgccg actgacgttc gccttccgct
    15661 ttcaggattt gcgactgttt ttcaccttcg gctttgagga tttccgcctg acggatccct
    15721 tccgcttcaa gaatgtaagc gcgtttggta cgttccgctt tcatctgcgc gttcattgaa
    15781 gagataagct ctgccggtgg gcgcacgtcg cgaatttcaa tacgggtgac tttaatcccc
    15841 cacgggttgg tggcttcatc gacaatacgc agcaggcgtg agttgatgct gtcgcgctga
    15901 gagagcattt cgtcaagttc cattgaaccc aacacggtac ggatgttagt catggtcagg
    15961 ttgatgatcg ccagctccag attgctgact tcataagccg cgcgcggcgc gtcaatcacc
    16021 tgaataaaac agactgcgtc gatggtaacg ttggcgttat ctttcgagat aacttcctgg
    16081 gaagggatat cgagcacttg ctccatcata ttgatcttgc gaccaatgcg atccataaac
    16141 ggcaccacca gactgagccc cggctgtaac gttttggtat agcgaccaaa acgttctacc
    16201 gtccattgat agccctgcgg cacgattttg acgcccgcgc cgacaatgac cagcgcgaca
    16261 aaaatgagaa tcgggataaa gataagcatc ggaaaaacct cctgttgtac cgtccataat
    16321 cagcaaaatt gctgcttgat taaacaaatt atacctgatt actgaaagag agttccccct
    16381 tattcctgcg aaggataaac tgtttttagt aaaaatcaga aaaagggaac agtgatgcag
    16441 gaaaatagtc ctttgcttca gctacaaaac gtaggatatc tggcgggtga tgcgaagatt
    16501 cttaataaca tcaatttttc gctgcgtgct ggcgaattta agttaattac cggtccttcc
    16561 ggttgtggca aaagtacgct gctaaaaata gttgcttcat tgatcagccc aaccagcgga
    16621 acgttactgt ttgaaggtga ggatgtcagc acactaaagc cagaaatcta ccgccagcaa
    16681 gtctcttact gcgcccagac gccgacgctg tttggcgata cggtatacga taatctgatc
    16741 tttccctggc agatccgtaa ccagcagcct gacccggcca tttttctcga ttttctcgaa
    16801 cgcttcgctt tgccggacag cattttgacg aaaaatatcg ccgagctatc tggtggtgaa
    16861 aaacaacgca tctcactgat tcgtaacctg caatttatgc cgaaggtttt attgctggat
    16921 gaaatcacca gtgcgctgga tgaaagtaat aaacataacg tcaatgagat gatccatcgt
    16981 tatgtgcgcg agcaaaatat tgccgtgctg tgggtgacac acgataaaga cgaaattaat
    17041 catgcggata aagtgattac actgcaaccg catgccggag aaatgcagga agcacgctat
    17101 gaactcgcat aatattacta acgaatcatt ggcactggca ttaatgctgg tggtggtggc
    17161 aatcttaatt agccataaag aaaaactggc gctggagaaa gatattttct ggagcgtcgg
    17221 gcgagcgata attcagctga ttattgtcgg ctatgtgctg aagtatattt tcagcgtgga
    17281 tgatgccagc ctgacattat tgatggtgtt atttatctgc tttaatgcgg cgtggaacgc
    17341 gcaaaaacgc agtaaatata ttgctaaagc ttttatctca tcgtttattg ccattacggt
    17401 cggggcggga attaccctgg cggtgctgat tctctctggg tcgattgaat ttatcccgat
    17461 gcaggtgatc cctatcgccg ggatgattgc cggtaacgcc atggtagcgg tggggttgtg
    17521 ttacaacaat ttagggcaac gggtcattag cgaacagcaa cagattcagg agaaactgag
    17581 tcttggtgcg acgccgaagc aggcttcagc gatattgatt cgcgacagta ttcgcgcggc
    17641 tttaattccg acggtcgatt cagcaaaaac ggttggctta gtgagtttac caggaatgat
    17701 gtccgggctg atatttgccg ggattgatcc ggtgaaggcg attaaatatc agattatggt
    17761 gacctttatg ctgctctcaa ccgccagctt gtcgaccatt attgcctgct atttaaccta
    17821 tcgtaagttt tataattcgc gccaccagtt ggtggtgacg caattgaaga agaaataatg
    17881 cgatgccgga tgcgcagcat cgcatccggc gttgtggttc atgtgccgat caacggaata
    17941 tcaatacaac aatgcataca gctggcggcg atacttcgac gccagtgcat cacccgtacc
    18001 cagcgcagcg aggatctcct ggaacgtttt acgcgtctga ccgtctgcgg cggtgagatc
    18061 tttacgcaga tgcccgaaca gcaactccag cgcctcttca ttgcgcccaa cctggtgcag
    18121 ttgcagagcc agttgcgtcg ccagtgcggc atcttctgga ttctccgcca cctgctgttg
    18181 caactgttga atttccggcg tatcagccgc ctgcttcagc agttcgattt gcgccaccag
    18241 cccctggtag cgggtgtcct gatcctgcaa cggaatggtt ttcagcaccg cttccgcatc
    18301 ttcagaacgg ttcagcgcaa tcagcgtttc tgccagcagc aggccgatct ccccgttctg
    18361 attcgacaac tgccaggcgt ctttcagcaa tggcagggca tcggtgtaat tgccttcctg
    18421 catcagttgc atcgcctgct gcgctttcag ctcttcttcg cgcggcagca ctttatccag
    18481 cagggcgcgg atcgcctctt ccggttgcgg cccctggaag ccatctaccg gttgcccgtt
    18541 ctggaacaga tacacggtcg gaatcgcgcg cagaccaaac tgggcggcaa tcatctgctc
    18601 cgcgtcacag tccagcttcg ccagaataaa ttgcccgttg tactgcgccg cgaggctttc
    18661 caggatcggg gttaactgca aacagtgctg gctacgttca gaccaaaaat agaacagcac
    18721 cggagtggtc atcgactgtt caagaacctg ttgcaggtta gattcgttaa tgttgacaat
    18781 attttctacg gacatggagt cgctctctgt tgtcgatatt ttctttgaca tgggggctta
    18841 agcgcgcgct tcaactcacc cctgcaatat tttgtccatc acgcgccccg gcagcaggcg
    18901 cttaagcacc attactgccc aggtcaccag cgttaccgga tagcgcatct tcggcttctc
    18961 gctaataaaa gcatggcgta ctttgtccac caccgcttcc ggtcccaacg taaagcgggc
    19021 ggcgatgccg ggattttcga ctggtttatc actttgcgtc tggttgacgt tgtcggtgaa
    19081 gcgagtacga atgggaccgg gttcgatcag gctgacttta attccgctgt ggcgtagctc
    19141 catgcgcagc gcgtctgacc acgcctccag cgcatattta ctggccgcgt aagcgccacg
    19201 acccggcgtg gagattaatc ccatcaccga tgatgtcatc acaatacgcc cttcaccgtg
    19261 cggtaacatc gcgggtaaca ggcgcatggt gagctggtgt gcgccgaaaa agttggcgga
    19321 aaactgctgt tccatctgcg cacggctgat ggtggaaagg gggccataca tgccgaatcc
    19381 ggcattgtta aagatcccat acagacaatt atcggtcagg gcgatcacct cgtctgctgc
    19441 gcgatcaaca ctttctggtg aatccagatc gatcaacacg ccggtaaatc ccatgttgtt
    19501 catacgctca acatcatccg gttttcggca acctgccagc acatgaaaac cctggcgttt
    19561 taattcgagc gcgctttcca ggccaattcc actggaacat ccggtaatta agaccgattt
    19621 ttgcataact ttacctgtca ggatctccgt tgctttatga gtcatgattt actaaaggct
    19681 gcaactgctt cgccatccag tcggcaataa acggctgggc gtcgcggttg ggatgaatac
    19741 cgtcatcctg catccattgt ggcttgaggt agacctcttc cataaaaaag ggcagcagcg
    19801 gaacatcaaa ctctttggcg agtttggggt aaatggcgct aaaggcttca ttataacggc
    19861 gaccatagtt tgcaggcaga cgtatttgca ttaacaatgg ttcagcgttg gcggctttga
    19921 catcctgcaa aatctggcgc agcgtttgct cggtttgctg tggctgaaaa ccacgcaaac
    19981 cgtcattgcc gcccagttca accagcaccc aacgcggctg atgctgtttc agcagagccg
    20041 gaaggcgcgc cagtccttgt tgcgaggtgt cgccgctgat gctggcatta actaccgacg
    20101 ttttactctg ccacttatca ttcaacaagg caggccaggc cgcgctggca gacattcgat
    20161 acccggcgct caggctatca cccagaatca ataacgtgtc cgctgcggcg gcacggaagg
    20221 ttaacaggac caggaacagg aagggcaaat gccagcggaa aacattgttg aagttcatca
    20281 tcttaagaag tccgtcggtc agggggagca tgaactctcc atcctcaccg gagttgagct
    20341 ggttgtcaaa cgtggcgaga ccattgcact ggtgggcgag tcgggatcgg gtaagtcaac
    20401 cttgctggcg atcctcgccg ggctggatga cggcagcagt ggcgaagtga gtctggtggg
    20461 acaaccgcta cataatatgg acgaagaagc gcgggcaaag ttgcgcgcga agcacgtagg
    20521 ctttgttttt cagtcattta tgttaattcc tacccttaac gcgctggaaa acgtcgagct
    20581 tccggctctg ctgcgcggtg agagtagcgc ggaaagtcgt aacggggcga aagcgttgct
    20641 cgaacagtta gggctgggta aacgtctgga tcatcttccg gcacagcttt ccggcggtga
    20701 acagcaacga gtggcgctgg cacgagcctt taatggtcga cctgatgtgc tgtttgccga
    20761 cgaacccacc ggcaaccttg accgccagac gggcgataaa attgccgacc tgctgttttc
    20821 cctcaaccgt gaacatggca ccacgttgat tatggtgacc cacgacctgc aactggcggc
    20881 acgctgcgac cgctgcttac ggctggtgaa cgggcagttg caggaggaag catgattgca
    20941 cgttggttct ggcgcgaatg gcgttcgccg tcgctattaa ttgtctggct ggcgctaagc
    21001 ctggcggtgg cctgcgtgct ggcgctgggc aatatcagcg atcgcatgga gaagggctta
    21061 agccaacaaa gccgggagtt tatggcgggc gatcgggcgt tgcgcagttc gcgcgaagtg
    21121 ccgcaagcat ggctggagga agcgcaaaag cgcggcctga aagtcggcaa gcagctgact
    21181 ttcgccacca tgacatttgc aggcgacaca ccgcagctgg cgaacgtcaa agcggtggat
    21241 gatatctacc cgatgtatgg cgatctgcaa actaatcccc ctggcctgaa accgcaggcg
    21301 ggcagcgtat tgctggcccc acgcctgatg gcactgctta acctgaaaac gggcgacacc
    21361 attgacgtgg gcgatgccac cttgcggatt gccggagaag tgattcagga accggattcc
    21421 ggttttaacc ccttccagat ggctccgcgt ctgatgatga atctggcgga tgtcgataaa
    21481 accggagccg tgcagccggg gagtcgggtc acctggcgct ataaattcgg cggcagtgag
    21541 aaccagctcg acagctatga gaaatggttg ttacctcagc ttaaacccga acaacgctgg
    21601 tacggtctgg aacaggacga aggcgcgctg gggcgatcga tggaacgctc gcaacagttc
    21661 ctgctgcttt cggcgcttct gaccttgctg ctggcagtgg cagcagtggc ggtagcgatg
    21721 aatcattact gtcgcagtcg ctacgatctg gtggcgatcc tcaaaacgct gggggcaggg
    21781 cgagcgcaac tgcgtaagct aatcgtcggt cagtggttga tggtgctgac gctttcagcc
    21841 gttaccggtg gggcgatagg cctgttgttc gaaaacgtgt tgatggtgct gctcaagccc
    21901 gttctgcctg ctgcactacc gccagccagc ctctggccgt ggctatgggc gctgggcacc
    21961 atgacggtca tctcgctgct ggtggggcta cgaccatacc gcttgttgct ggcaacgcag
    22021 cctttacgcg tattacgtaa tgatgtggta gcgaacgtct ggccgctgaa gttttatctg
    22081 ccgattgtca gtgtagtggt tgtgctgctg ctcgccggat taatgggggg cagtatgctg
    22141 ctctgggcgg tgctggcggg cgcggtagta ctggctttgc tgtgcggtgt gctgggctgg
    22201 atgctgctga atgtacttcg ccgcatgacg ctgaaatcgc tgcctctgcg cctggcggtc
    22261 agccgcctgt tacgtcagcc gtggtcaacg ttaagccagc tttcggcatt ttcgctctcc
    22321 tttatgctgc tggcactgct gctggtgttg cgtggtgatc tgctcgaccg ctggcagcag
    22381 cagctacctc cagaaagccc gaactacttt ttaattaaca tcgccacaga acaggtagca
    22441 ccgctaaaag cgttcctcgc ggaacatcag atagtcccgg aatcgtttta tccggtggtg
    22501 cgggcgcggc tgacggcgat taacgataag ccgacagaag gtaatgaaga tgaggcgctt
    22561 aaccgcgaac tcaatcttac ctggcaaaat acgcggcccg atcataatcc gattgtcgcc
    22621 ggtaactggc cgccaaaagc cgatgaagtg tcgatggaag aggggctggc gaaacgctta
    22681 aaggttgccc tcggtgatac cgtgactttt atgggcgata cccaggagtt ccgcgctaaa
    22741 gtgaccagcc tgcgcaaagt ggactgggaa agtctgcggc ctaatttcta ttttattttc
    22801 ccggaagggg cattagacgg gcaaccgcag agctggctta ccagtttccg ctgggagaat
    22861 ggcaacggca tgttgacgca actcaaccgc cagttcccga ccattagcct gttagacatt
    22921 ggcgcgattt taaaacaggt cggtcaggtg ctggagcagg taagtcgggc gctggaggtg
    22981 atggtggtgc tggtcaccgc ctgcggtatg ttgctgttgc tggcacaggt gcaggtgggt
    23041 atgcgtcagc gccaccagga gctggtggtg tggcgcacac tcggtgcggg gaaaaaactg
    23101 ctgcgtacca cgttgtggtg tgagttcgcc atgcttgggt ttgtttccgg cctggtggcc
    23161 gcaattggtg cggaaacggc gctggcagtg ttgcaggcga aagtgtttga ttttccgtgg
    23221 gagccagact ggcgattgtg gattgttctg ccgtgcagcg gagcgctgct gctgtcgctt
    23281 ttcggcggct ggctgggtgc gcgactggtt aagggtaagg cgctgttcag gcagtttgcg
    23341 gggtgatgac aggtgttaga ttgcctgatg cgctacgctt atcaggccta cgaagagcgt
    23401 gcaatatatt gattttgcaa gattgtgtag gccggataag gcgtttacgc cgcatccggc
    23461 agttgtgcgg cggtgcctga tgcgacgctg gcgcgtctta tcatgcctac aaaactgcac
    23521 tcaaccgtag gccggataag gcgtttacgc cgcatccggc agttatgcgc agatgcctga
    23581 tgcgacgctt tgcgcgtctt atcatgccta caaaactgaa cttaaccgta ggccggataa
    23641 ggcgtttacg ccgcatccgg catgaacagt gtgcattttg gcaataaata tgaaaccaac
    23701 tgtcatgtcg gtttttttaa aggccgaagg tggtaaacct tcggccttag ctacgtttcc
    23761 tcagttattc aataaactga cggatcagtt ccagcagcgc gattagcgcc ttaagaacga
    23821 tgagaacttt atcgagaaaa gttttcatcc gttgctccct taagggagaa agacaatcac
    23881 agcttaccct tacgttgcct gtaactatga ttttcttcat gtgctagatg aggactgcct
    23941 ctctccaaat tagagcacca gaatcagcac cacctgtatc ctgaccagga cttaaaagta
    24001 cttttccgca ctgcggtaat cccttgaggc agtatcacta ctagcgtcgg gattgtatcg
    24061 ccaacatagc tgtttgaata tgtaggcatc ctgacttata attcactcgc tagatgagga
    24121 ctgtttcatt tctgcccgga accaccaagt cttgggcgga aaaaataaaa aaaccgactg
    24181 tcatgtcggt ttttttatat ctgtattctg gtgcaaccat tctgttctca cgacaatcgc
    24241 cgtttcagaa taccgcccat cactgcgcct taacccattc cgccacttcc gcccactcac
    24301 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatc gggtcgtaat
    24361 attcttcaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    24421 gcgttgtcag tgctgcatcc agccttgcac tcagttcgtt atagcgctgt agccccagcc
    24481 gacgtttaat cgccgaaagt ccgtgatgca ggtattcgca atactcctgc cagccctgtt
    24541 cgtcgccgta cgcgtgggta aaatcatgat gcatacgcaa gaaatactct tcgttcaggc
    24601 gctcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    24661 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    24721 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    24781 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    24841 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    24901 cggtacaacc gccaatcagc actatcggtt tttgtgacag ttcaatgatc gcctgaatcg
    24961 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    25021 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    25081 gcggattttg caggcacgct gcccgccagg cgtccatgcg ctgctgacga atttcacccg
    25141 ctaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    25201 caacggcggc gcgttcatcg ttattcatta acggcagatt gatagcggcg ggcattgcgc
    25261 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    25321 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    25381 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtgggttgcc
    25441 gaaaatttct aaaaatccgc tgatttccgg cctgcgctgg gtaaacaagg tcacaatatc
    25501 ttctaccgct ttgccgctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    25561 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagcga
    25621 ttttggcaaa aaaccaatgc caacgcccgc aagatgggcg gcgattttcg tttccatatc
    25681 aggaacaata atctcttttt gccctggcaa tcgccaggcg acgcgtttgg ttaaggtgcg
    25741 ggcgctgtct tcaatattga ccgccggaaa gcgccgcaac tgcgcttctg ttagcggctc
    25801 ttcaacgttc gccagcggat gatccgccgc catgacaaag cgccattgca ccgatcctaa
    25861 gggatcaaga ctaaaggtat ttgccagcgc ctcggttccc gtgacgccga tagccagtga
    25921 aaaaccttcg tacaatagcg aatcccagac gcccatatag atttgtcggg agatgtgaaa
    25981 ctgggtaaag gggtaacgtt cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    26041 gtagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    26101 cagctcgctt ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    26161 ctcgccagcc gctgtcaacg tcacgctacg agtcgtacgg aaaaacagcg ctactccggt
    26221 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    26281 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    26341 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    26401 gatttatcag tatttttaca tgataaccct gttcaatttg tggactaaat ctagttttgg
    26461 aaaaatattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    26521 gaaaccaagc tgaccggtcg gcgatggttg aacggaatta tgttacaagg acaaaaagat
    26581 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    26641 aactcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    26701 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    26761 ggcgaatctg ccgctgacca tttacgaact cgaacgtcat ccgctgggca ctcaggcctt
    26821 tatcccgatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    26881 cctgtcaacg ctgcgggcgt ttatcaccaa cggcgaacag ggagtgaatt accatcgtaa
    26941 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    27001 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    27061 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    27121 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    27181 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    27241 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    27301 tccacgacct ttcgcttgct gaaggtttta caggcagcgg attttgtcta tcaggacagt
    27361 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    27421 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    27481 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcag tattaattgg tcagttagag
    27541 tgtaaatcaa tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    27601 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    27661 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    27721 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    27781 ctgaattgca tagcttcagc aatttacgat gatgtcggta gcgttgttgc cgctatctcc
    27841 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    27901 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    27961 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    28021 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    28081 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca atcaatccgt
    28141 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    28201 gtgcttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    28261 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattct gcttctgctg
    28321 attccattcc tattctgtgt attactggcc aggctccgcg cgcccgtctg cataaagaag
    28381 atttccaggc tgttgatatc gaagctatcg ccaagccggt aagtaaaatg gcggttacag
    28441 tacgtgaagc ggcgctggtg cctcgtgtgt tgcaacaggc atttcacctg atgcgttctg
    28501 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    28561 ttgatcctga tatgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    28621 tcgaaaaagc tgtagaaatg ttaatccagg cccaacgtcc ggtgattgtt gccgggggcg
    28681 gggtaattaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    28741 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    28801 ggatggtggg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    28861 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    28921 ccgaagggcg caaaatcgtt catatcgata ttgagccgac gcaaattggt cgcgtgctgt
    28981 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    29041 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    29101 agcagcgcaa acgcactttg ctgcgcaaaa cccatttcga caacgtgccg gtgaaaccgc
    29161 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgctat gtcaccacca
    29221 ttggtctgtc gcaaattgcc gctgcgcaaa tgctgcatgt ctttaaagac cgccactgga
    29281 tcaactgtgg gcaggcaggc ccgctgggct ggaccattcc ggcggcgctg ggggtttgtg
    29341 ccgctgatcc agagcgcaaa gtggtggcga tttccggtga cttcgacttc cagttcctga
    29401 ttgaagaatt ggccgttggc gcacagttca acattccgta catccatgta ctggtcaata
    29461 acgcttatct ggggctgatt cgccagtcgc agcgcgcgtt tgatatggac tactgcgtgc
    29521 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    29581 aagtagcgga aggtttaggt tgtaaagcga ttcgcgtctt caaaccggaa gatattgcgc
    29641 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    29701 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    29761 ttgaagatat cgccgataac gcagcggacg caccgactga aacctgcttc atgcactatg
    29821 aataagggag ataaataatg ttacgtttct ctgctaattt atcgatgtta tttggagaat
    29881 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    29941 tgtttcctta tgactacgac attgaagaat taaaacaggt gctggcgagt aataaactcg
    30001 aacatacgct gcacaattta ccggcgggtg actgggcggc gggggagcgc ggtattgcct
    30061 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt cgttatgccc
    30121 gtgcgctggg taataaaaaa attaactgtc tggtcggtaa aacgccggct ggtttcagca
    30181 gtgaacagat tcacgcaacg cttgtagaaa acctgcgtta tgccgcgaat atgctgatga
    30241 aagaagatat tttattactg attgaaccta ttaaccattt tgatattcct ggtttccatc
    30301 tcaccggaac tcggcaggcg ctgaaattga ttgatgatgt tggttgctgc aatttgaaaa
    30361 ttcagtatga catttatcac atgcagcgga tggaaggtga attaaccaac accatgactc
    30421 agtgggctga taaaattggt cacctgcaaa ttgccgataa tccgcatcgc ggcgaaccgg
    30481 gaaccggaga aattaattat gattatctct ttaaggtaat cgaaaattct gactacaacg
    30541 gttgggttgg gtgtgaatat aaaccccaaa ccaccacgga agccggttta cgctggatgg
    30601 atccgtaccg ttaaaacgta acgctgttca ggcaatgctt ttttaggccg ctaagttggc
    30661 aggggatcgt gttgtctgaa ttcaggaaaa gcgaaattta aaagaggtta attatgaaac
    30721 tgggatttat tggcttaggc attatgggta caccgatggc cattaatctg gcgcgtgccg
    30781 gtcatcaact gcatgtcaca accattggac cggttgctga tgaattactg tcactgggtg
    30841 ccgtcagtgt tgaaactgcg cgccaggtaa cggaagcatc ggacatcatt tttattatgg
    30901 tgccagatac accacaggtt gaagaagttc tgttcggtga aaatggttgt accaaagcct
    30961 cgctgaaggg caaaaccatt gttgatatga gctccatttc cccgattgaa actaagcgtt
    31021 tcgctcgtca ggtgaatgaa ctgggcggcg attatctcga tgcgccagtc tccggcggtg
    31081 aaatcggtgc gcgtgaaggg acgttgtcga ttatggttgg cggtgatgaa gcggtatttg
    31141 aacgtgttaa accgctgttt gaactgctcg gtaaaaatat caccctcgtg ggcggtaacg
    31201 gcgatggtca aacctgcaaa gtggcaaatc agattattgt ggcgctcaat attgaagcgg
    31261 tttctgaagc cctgctattt gcttcaaaag ccggtgcgga cccggtacgt gtgcgccagg
    31321 cgctgatggg cggctttgct tcctcacgta ttctggaagt tcatggcgag cgtatgatta
    31381 aacgcacctt taatccgggc ttcaaaatcg ctctgcacca gaaagatctc aacctggcac
    31441 tgcaaagtgc gaaagcactt gcgctgaacc tgccaaacac tgcgacctgc caggagttat
    31501 ttaatacctg tgcggcaaac ggtggcagcc agttggatca ctctgcgtta gtgcaggcgc
    31561 tggaattaat ggctaaccat aaactggcct gatacccgca ataaaaatgg ccgatatcag
    31621 aaaatgaatc ggccagcaat attaaaaaag aaagcagcca aagatgttgc ttcagtatta
    31681 aaaataatat tttttatttt atttgttcct catagctaga ttaaaacaac gttattcgat
    31741 acgtgaaatt aagagggatt tatggaacat cagagaaagc tattccagca acgcggctat
    31801 agcgaagatt tattgccgaa aacgcaaagc cagcggacct ggaaaacatt taactatttt
    31861 accttatgga tgggttcggt tcataacgtt cccaattatg tgatggtcgg cggctttttt
    31921 attctcggct tgtctacctt tagtattatg ctggcaatta tcctcagcgc ctttttcatt
    31981 gccgcggtaa tggtattaaa cggtgctgcg ggcagtaaat acggcgtacc gtttgccatg
    32041 atcctgcgtg cttcttacgg cgtacgtggc gcactgtttc ccggattatt aagaggtggt
    32101 attgctgcca ttatgtggtt tggcctgcaa tgttacgccg gatcactggc ctgcttgatc
    32161 ctgattggca aaatctggcc gggattttta actctcggtg gtgattttac cttgttaggc
    32221 ctttcgctac cgggcttaat tactttctta ctcttctggc tggttaacgt tggtattggt
    32281 tttggcggtg gcaaagtttt aaataaattc actgccattc ttaacccgtg catctatatc
    32341 gttttcggcg gcatggcgat ttgggcgatt tcactggtcg ggatcggtcc aatctttgac
    32401 tacattccga gcggtattca gaaagcagaa aacagtggat tcttgttcct ggtggtgatt
    32461 aacgcggtag ttgcggtctg ggcggcaccg gcggtgagcg catccgactt tacgcaaaac
    32521 gcccactcgt ttcgtgagca agcgctgggg caaacgctgg gtttagttgt ggcctatatt
    32581 ctgtttgcgg tcgctggggt atgtattatt gccggagcca gtattcacta cggcgctgac
    32641 acctggaacg tgctggatat tgttcagcgt tgggacagcc tgtttgcctc gttctttgcg
    32701 gtactggtta ttctgatgac gactatttcc actaacgcca ccggtaatat tattccggcc
    32761 ggttatcaga ttgctgccat tgcaccgaca aaactgacct ataaaaacgg cgtactgatt
    32821 gccagtatta tcagcctgct gatctgcccg tggaaattaa ttgaaaatca ggacagtatt
    32881 tatctgttcc tcgatatcat cggcggaatg ctgggtccgg taattggtgt catgatggca
    32941 cattattttg tggtgatgcg tgggcaaatt aatcttgatg aactgtacac cgcacctggc
    33001 gattataaat attacgataa cggttttaac ctcaccgcgt tttcagtaac tctggtggcc
    33061 gttattttat ctcttggcgg taagtttatt ccctttatgg aaccgttatc gcgtgtttca
    33121 tggtttgtcg gcgtcatcgt cgcctttgcg gcctacgcct tattaaagaa acgtacaaca
    33181 gtagaaaaaa caggagagca aaaaaccata ggttaattaa tcccgatatt gaacattgag
    33241 ttaaaaacca atctgtattt tacaaggagt ttgttatgtc ttttgattta atcattaaaa
    33301 acggcaccgt tattttagaa aacgaagctc gcgttgtaga tatcgccgtt aaaggcggga
    33361 aaattgctgc tatcggtcag gatctgggcg atgcaaaaga agttatggat gcgtctggtc
    33421 tggtggtttc gccgggcatg gttgatgcgc acacccatat ttctgaaccg ggtcgtagcc
    33481 actgggaagg ttatgaaacc ggtactcgcg cagcggcaaa aggtggtatc accaccatga
    33541 tcgaaatgcc gctcaaccag ctgcctgcaa cggttgaccg cgcttcaatt gaactgaagt
    33601 tcgatgccgc taaaggcaag ctgactatcg atgcggcaca actcggtggc ctggtgtctt
    33661 acaacatcga ccgtctgcat gagctggatg aagtgggcgt tgtcggcttc aaatgcttcg
    33721 ttgcgacctg tggcgatcgc ggtatcgaca acgacttccg tgatgtaaac gactggcagt
    33781 tcttcaaagg tgcgcagaag ctgggcgaac tggggcagcc ggtgctggtg cactgcgaaa
    33841 acgcgctgat ctgtgacgca ctgggcgaag aagcgaaacg tgaaggtcgc gtaactgccc
    33901 atgactatgt ggcttcgcgt ccggtattta ccgaagtgga agcgattcgc cgcgtgctgt
    33961 atctggcgaa agttgccggt tgccgtctgc acgtttgcca tatcagcagc ccggaaggcg
    34021 ttgaagaagt gactcgtgca cgtcaggaag gtcaggacgt tacttgtgaa tcctgcccgc
    34081 attactttgt actggatacc gatcagttcg aagaaatcgg cactctggcg aagtgttcac
    34141 cgccgatccg cgatctggaa aaccagaaag gcatgtggga aaaactgttt aacggtgaaa
    34201 tcgactgcct ggtttccgac cactcaccat gccctccgga aatgaaagcc ggcaacatca
    34261 tggaagcatg gggcggtatt gccggtctgc aaaactgtat ggacgtgatg ttcgatgaag
    34321 cggtacagaa acgcggaatg tctctgccaa tgttcggcaa attaatggcg actaacgcag
    34381 cagatatttt cggtctgcag caaaaaggcc gtatcgcccc aggaaaagat gccgacttcg
    34441 tcttcattca gccgaatagc agctatgttc ttaccaatga cgatctggaa tatcgccaca
    34501 aagtcagccc gtatgttggc cgtaccattg gcgcgcgtat cacgaaaacc atcttacgtg
    34561 gtgatgtgat ttacgacatt gaacagggct tccctgttgc gccgaaaggt caatttatcc
    34621 ttaaacatca gcagtaatct ggcccctgca atgcccgtcc ttgcggcggg cattctccgg
    34681 ttaaggtgtg tttatgttca attttgcagt gagccgcgaa agcctgttat caggatttca
    34741 gtggtttttc tttatttttt gcaacacggt tgtggttcct cctacgctac tttctgcttt
    34801 tcagttgccg caaagtagcc tgcttacgct cacgcaatat gcttttcttg ctaccgcact
    34861 ggcctgcttc gctcaggcgt tctgcggtca tcgtcgcgct attatggaag ggccaggtgg
    34921 cctgtggtgg ggaaccatcc ttactatcac ccttggcgaa gcatcgcgcg ggacaccgat
    34981 caacgatatc gccaccagcc tggcagtggg gattgcactc tccggcgtgc tgacggtgtt
    35041 gattggtttt agcggattag gccatcgcct ggcgcggtta tttacgccgt cggtgatggt
    35101 cttgtttatg ttgatgctgg gcgcgcagct gaccactatc tttttcaaag gtatgctcgg
    35161 gctgccgttt ggcatagccg acccgaattt taaaattcag ttaccgccgt tcgcgctctc
    35221 ggtggcggtg atgtgcctgg tactggggat gattatcttc ctcccgcaac gttttgcccg
    35281 ttatggcctg ctggtcggca ccataaccgg ctggttgttg tggtactttt gctttccttc
    35341 ttcgcactcg ctctccggtg agttgcactg gcagtggttc ccgctcggca gtggcggtgc
    35401 tttgtcgccg ggaattattc tgacggcggt gattacaggt ctggtaaata tcagcaatac
    35461 ctacggtgcg attcggggca cggatgtttt ttatccgcag cagggcgcgg ggaatacgcg
    35521 ttatcgtcgt agctttgtgg cgaccggatt tatgacactg ataaccgtac ctctggcggt
    35581 aattccattt tcaccgtttg tttcatccat tggtttatta acccagactg gcgattacac
    35641 gcggcgttca tttatttatg gcagcgttat ttgccttctg gtggcgctgg ttccggcact
    35701 cacacgactg ttttgcagta tccctttacc cgtgagtagt gcggtcatgc tggtttctta
    35761 tctgccttta ctcttttccg cgctggtgtt tagccagcaa ataacgttta ccgctcgcaa
    35821 tatttatcga ctcgcattgc cgttatttgt cggcatattt ttaatggcat taccgcctgt
    35881 gtatctgcaa gaccttccat taacgctgcg tcctctgctc agtaacggct tactggtcgg
    35941 gattttactg gctgttctta tggataatct tattccgtgg gaacgcatcg aataatttgt
    36001 tgaaaaagga ttgataatga agattgtcat tgcgccagac tcttttaaag agagcttaag
    36061 tgcagaaaaa tgttgtcagg caattaaagc cgggttttcg accctctttc ccgatgcgaa
    36121 ctatatctgt ttgccgatag cggatggcgg cgaagggacg gtggatgcga tggtcgccgc
    36181 gacgggcggc aacatcgtga cgcttgaagt ctgcgggccg atgggcgaaa aagtgaatgc
    36241 tttttatggc cttaccggcg acgggaaaac ggcggtgatt gagatggcgg cagcaagtgg
    36301 cctgatgctg gtcgcgcctg aaaagcgtaa tccgttgctg gcctccagtt ttggtacggg
    36361 ggagttaatt cgtcatgcgc tggataacgg cattcgccat attattctcg gcattggtgg
    36421 cagtgcgacg gtcgacggtg gtatgggcat ggcgcaggcg ctcggtgtgc gtttccttga
    36481 tgccgacggt caggtgctgg tggctaacgg cggtaattta gcgcgcgtgg caagcattga
    36541 gatggatgaa tgcgatccgc gtctggcgaa ttgccatatt gaagtagcat gtgacgttga
    36601 taacccgctg gtaggggcac gcggcgcggc ggcggtgttt ggcccgcaaa aaggggcaac
    36661 gccggaaatg gtcgaagagc tggaacaggg gctgcaaaat tacgcccgtg ttttacaaca
    36721 gcaaactgaa atcaacgtct gccagatggc gggcggcggc gctgcgggcg gtatgggtat
    36781 tgcggcggcg gtatttctca atgcggatat taaaccaggt attgagattg tgttgaatgc
    36841 ggtcaatctt gcgcaggcag tgcagggcgc agcactggtg attaccgggg aagggcgcat
    36901 cgactcgcaa acggcaggcg gtaaagcgcc gctgggtgtg gcgtcggtgg cgaagcagtt
    36961 taatgtaccg gtgattggga ttgctggcgt attgggtgat ggcgtggaag tggtgcacca
    37021 gtacggcatt gatgcggtat tcagcatttt gccacgtctg gcacctttag ccgaagtgct
    37081 cgccagcggt gaaaccaatc tcttcaacag cgcacgaaat attgcctgcg ccattaaaat
    37141 aggtcaggga attaaaaact gacccttacc tttaaagcgg atgcgattta tatcgcatct
    37201 ttttttacct gaatttacta caaaaaaagc cacggtataa accgtggcaa aatccaacat
    37261 agctaaaaat aatcaggcga gtggtatgac ttaaatctct acgtcgcggt tgcaatcttt
    37321 cgagtaaata tagctgaacg cttcaccacg ccctacacca taaccagcct gtaaagaata
    37381 agcgcccata aagatgtaat cgcctttttt caccgggatc cagttattgt cgaggttata
    37441 aaccccctga ccggaaagaa tataggcacc gtgttcctga acatgtgttt cgatataacc
    37501 gtggctggca cctggtgcaa aagagaggat atgcatgttc atatcaaaac ctaactcttt
    37561 gggcagaaaa tccagcagga tcacatcatc cataccttca tagtgaatgc gttccagttc
    37621 gctggcattg ccagaaacca gccacggtgc atggccttct accggaacat agcgacgctt
    37681 atataaaaag atttggctgt cttcggcctg ggcgttaaca aacgtcatta aggagcctgg
    37741 tgggcaataa agatagccac
//
LOCUS       DAEVZA010000034        35806 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEVZA010000034 DAEVZA010000000
VERSION     DAEVZA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.034"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(60..1391)
                     /locus_tag="LAF92_001781"
     CDS             complement(60..1391)
                     /locus_tag="LAF92_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBI9790801.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(1666..3030)
                     /gene="tdcG"
                     /locus_tag="LAF92_001782"
     CDS             complement(1666..3030)
                     /gene="tdcG"
                     /locus_tag="LAF92_001782"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBI9790802.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFTRIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(3102..3491)
                     /locus_tag="LAF92_001783"
     CDS             complement(3102..3491)
                     /locus_tag="LAF92_001783"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBI9790803.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(3505..5799)
                     /gene="tdcE"
                     /locus_tag="LAF92_001784"
     CDS             complement(3505..5799)
                     /gene="tdcE"
                     /locus_tag="LAF92_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBI9790804.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(5833..7041)
                     /gene="tdcD"
                     /locus_tag="LAF92_001785"
     CDS             complement(5833..7041)
                     /gene="tdcD"
                     /locus_tag="LAF92_001785"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBI9790805.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(7067..8398)
                     /gene="tdcC"
                     /locus_tag="LAF92_001786"
     CDS             complement(7067..8398)
                     /gene="tdcC"
                     /locus_tag="LAF92_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBI9790806.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(8420..9409)
                     /gene="tdcB"
                     /locus_tag="LAF92_001787"
     CDS             complement(8420..9409)
                     /gene="tdcB"
                     /locus_tag="LAF92_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBI9790807.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(9508..10446)
                     /gene="tdcA"
                     /locus_tag="LAF92_001788"
     CDS             complement(9508..10446)
                     /gene="tdcA"
                     /locus_tag="LAF92_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBI9790808.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            10635..10979
                     /gene="tdcR"
                     /locus_tag="LAF92_001789"
     CDS             10635..10979
                     /gene="tdcR"
                     /locus_tag="LAF92_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBI9790809.1"
                     /translation="MTGITIFYGDNIIRYVVNIKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            11235..11774
                     /locus_tag="LAF92_001790"
     CDS             11235..11774
                     /locus_tag="LAF92_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790810.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            11796..12983
                     /locus_tag="LAF92_001791"
     CDS             11796..12983
                     /locus_tag="LAF92_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HBI9790811.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNALQPGGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQKISTGSINVNCPLKYIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNPFKLNSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LTSISDMYSEEKIKFVESCLNELGDITEEQLKTMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMIDFSSEKIHDYIDIIIEDYENIKENAYLVIPQINYTMD
                     LNIEDSSSEELLSDNTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYTYNYDLL
                     NAI"
     gene            complement(13378..13754)
                     /gene="rnpB"
                     /locus_tag="LAF92_001792"
     ncRNA           complement(13378..13754)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LAF92_001792"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(13787..14932)
                     /gene="garK"
                     /locus_tag="LAF92_001793"
     CDS             complement(13787..14932)
                     /gene="garK"
                     /locus_tag="LAF92_001793"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBI9790812.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(15029..15919)
                     /gene="garR"
                     /locus_tag="LAF92_001794"
     CDS             complement(15029..15919)
                     /gene="garR"
                     /locus_tag="LAF92_001794"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBI9790813.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(15949..16719)
                     /gene="garL"
                     /locus_tag="LAF92_001795"
     CDS             complement(15949..16719)
                     /gene="garL"
                     /locus_tag="LAF92_001795"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBI9790814.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(16735..18069)
                     /gene="garP"
                     /locus_tag="LAF92_001796"
     CDS             complement(16735..18069)
                     /gene="garP"
                     /locus_tag="LAF92_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBI9790815.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            18444..20015
                     /gene="garD"
                     /locus_tag="LAF92_001797"
     CDS             18444..20015
                     /gene="garD"
                     /locus_tag="LAF92_001797"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBI9790816.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            20164..20499
                     /gene="prlF"
                     /locus_tag="LAF92_001798"
     CDS             20164..20499
                     /gene="prlF"
                     /locus_tag="LAF92_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HBI9790817.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            20499..20963
                     /gene="yhaV"
                     /locus_tag="LAF92_001799"
     CDS             20499..20963
                     /gene="yhaV"
                     /locus_tag="LAF92_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HBI9790818.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(21018..21827)
                     /gene="agaR"
                     /locus_tag="LAF92_001800"
     CDS             complement(21018..21827)
                     /gene="agaR"
                     /locus_tag="LAF92_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBI9790819.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPPVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            22076..23356
                     /gene="kbaZ"
                     /locus_tag="LAF92_001801"
     CDS             22076..23356
                     /gene="kbaZ"
                     /locus_tag="LAF92_001801"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBI9790820.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLAESLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            23379..23852
                     /gene="agaV"
                     /locus_tag="LAF92_001802"
     CDS             23379..23852
                     /gene="agaV"
                     /locus_tag="LAF92_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBI9790821.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKTAGVECFVQGVPTEPAVDLFKLL"
     gene            23863..24642
                     /locus_tag="LAF92_001803"
     CDS             23863..24642
                     /locus_tag="LAF92_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBI9790822.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            24632..25510
                     /locus_tag="LAF92_001804"
     CDS             24632..25510
                     /locus_tag="LAF92_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBI9790823.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            25528..25962
                     /locus_tag="LAF92_001805"
     CDS             25528..25962
                     /locus_tag="LAF92_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBI9790824.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            25959..27092
                     /gene="nagA"
                     /locus_tag="LAF92_001806"
     CDS             25959..27092
                     /gene="nagA"
                     /locus_tag="LAF92_001806"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBI9790825.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            27442..28596
                     /locus_tag="LAF92_001807"
     CDS             27442..28596
                     /locus_tag="LAF92_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HBI9790826.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            28609..29469
                     /gene="kbaY"
                     /locus_tag="LAF92_001808"
     CDS             28609..29469
                     /gene="kbaY"
                     /locus_tag="LAF92_001808"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HBI9790827.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            29636..30112
                     /gene="agaB"
                     /locus_tag="LAF92_001809"
     CDS             29636..30112
                     /gene="agaB"
                     /locus_tag="LAF92_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBI9790828.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            30151..30954
                     /gene="agaC"
                     /locus_tag="LAF92_001810"
     CDS             30151..30954
                     /gene="agaC"
                     /locus_tag="LAF92_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HBI9790829.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            30944..31735
                     /gene="agaD"
                     /locus_tag="LAF92_001811"
     CDS             30944..31735
                     /gene="agaD"
                     /locus_tag="LAF92_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HBI9790830.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            31736..32491
                     /locus_tag="LAF92_001812"
     CDS             31736..32491
                     /locus_tag="LAF92_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HBI9790831.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            32891..33475
                     /locus_tag="LAF92_001813"
     CDS             32891..33475
                     /locus_tag="LAF92_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI9790832.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            33555..34250
                     /locus_tag="LAF92_001814"
     CDS             33555..34250
                     /locus_tag="LAF92_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI9790833.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            34280..>35806
                     /locus_tag="LAF92_001815"
     CDS             34280..>35806
                     /locus_tag="LAF92_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI9790834.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     F"
BASE COUNT         9406 a         9001 c         8508 g         8891 t
ORIGIN      
        1 tgccataacg gatttaaagt cgaatcaaac atattaaaaa ccttaaaatt tcaggtaaat
       61 caagaaaatg ccaggaacgg agaaacacaa agcaacaaac cagtgacgat aatcaggtac
      121 agagacatcc ctttgtattt gtgcaatgcc ggtactttgt aaaccagcca cgccgggatc
      181 aggcacccta ccatgccgaa aatcgggcta cagatagagg tgaagctcaa caccggtgcg
      241 ttcagtacga tggcgctcca ggccagcaaa atggcgaaaa tcatgatgcc gcgctgaaca
      301 agattttcgt taatcttctc ggcaggcatc ttgcgacgca ggatgttcat tacgatccct
      361 tgcgttgctt cgcgaaagcc taaatagacg ccaaagaatg cagtcatgac agcaaagata
      421 ttgagaatga cgctgaccac tttcacccac gctgcgccgt caccgctaat aaactgtgcg
      481 gcaatcgcca gcgcggaaat attctgctca taggctttta ccgcttcgtc atgtcccatc
      541 gccagcgtga acgacacggc gtagaaaaag acggtgacaa acaaaatgcc aaacgcgata
      601 ttcattgccc gcaatgcttt atgccgcgcc acttcaattg atttttcccg cgagcgataa
      661 gagatcacca tcgggcttaa cgtctggata aacagaatcg acgtcagggt aaacggcagc
      721 gtaataatgg cgtttttcac cagcagcccc agcggcggta gcgaaccgac gttatacaga
      781 tgccacattc ccaccatcga cacgcccagc gccgcgacca ccagcagctt ggtcagcacc
      841 atgccggtcg aaattttgaa taacaatttc tcgccgcgtg aagagatcgc caccagaatg
      901 caaatcagta ccagaccata aaagggactg tctgacagca acccttccgt cacgccgaag
      961 gtatgcagat aggaagcact atcgttggtg atggcggtgg aatagacgaa catccaaatc
     1021 accagcatta cgaaatagag cgcacctaac aggatgcccc agtttttacc taaataaccg
     1081 ctaatgacac tcgggtaatc tttacattct ggtgattctg ccagcgtatt aataaacaac
     1141 cgctgaaaca gatacattgc cgggtaacca atcaccgatg agagcaaaaa tacccacaat
     1201 cccatcaaac cgacctgcac cgggagaaaa acaatccccg cgccaatcgc catcccgata
     1261 ctcataatca cccagccggt gtcagtactg tcgaatttga tcgcttctcg ccactcgctc
     1321 tcactcattc cagcacgacc cgccggggtc gaagcgtctg caatgacgcc tttattcgat
     1381 gcaatttcca taatttctcg ctcaatattt tgtagggctt attatttttt tccgagccgc
     1441 atcaaggcga tatgcggtct acgtttttgc aggcgagtta ttagaataaa gaaatgatac
     1501 gcgcgaggtc agagaaaatc ttcacaatca agactttgaa ataacggaga tggataagaa
     1561 ttttctacta aattaatcgc aagagaatat tcattctcta tttgcggcag atcacaaaaa
     1621 aaaggtgcac atttgtgcac ccaaggatga aagctgacag caatgtcagc cgcagaccac
     1681 tttaatggcc agtcctccgc gtgatgtttc gcggtattta tcgttcatat ctttgccggt
     1741 ttcatacatc gtctcgatca ctttatcgag tgaaacacgc ggtgccgagg tgcggcgcat
     1801 cgccatccgc gcggcgttta ctgctttcac ggcattaatg gcattacgtt caatgcacgg
     1861 gatttgtacc tgtccggcaa ccggatcgca ggtcagccca aggttatgct ccatcgcgat
     1921 ttccgccgca ttgcatacct gcgccggact gccgcccagt agttcagtta accctgccgc
     1981 cgccattgaa caggccacgc caatctcccc ctgacagccg acctccgcgc cagagatgga
     2041 ggcgttcatt ttatacagcg cgccaatagc ccccgctgcc agaaaatagc gggcaattga
     2101 ccgctcgttt accggacgac ggaacttatc gtaataagcc agcactgccg gaataatgcc
     2161 gcacgcaccg ttagtcggtg ccgttaccac gcgcccgcca gctgcgtttt cttcactaac
     2221 cgccagcgcg tacatgttga tccagtcgat gacattcatc ggatcgttag agatgttatc
     2281 gctagaaacc agctgacgac gcagcgctac ggcacgacgc ggcacattga gcggaccagg
     2341 cagcacgcct tcagtgttca tcccgcgttc aataccgtca tgcatcactt gccagatacg
     2401 ggtaaaccct gcgtcaattt ccgctttact gcgcagcgct agctcgttgt gcatcatcag
     2461 gccagatata gacaggccgt tgtaatcaca cattttcagc agttcacctg ctgagtggaa
     2521 atcgtaaggt acggacgttt cgacatcgtg cgacaggccg aagtgttctt cttcgacaat
     2581 aaacccgccg ccgacagagt aataggtttt acttaatagc gcttcctgtc ccttccaggc
     2641 agtgatccgc attccgttct catggcgagg caacatttcg ggatggaaga taatgttctt
     2701 tgctacagga aaatcaacaa tatgcgcacc agatgccact ggcagccgcc cgctgcgcgt
     2761 tactaactct ataaatgcag ggatctcatc aatgacaaca tcctgcggac tgtttcctgc
     2821 cagtcccatg ataatggcga catccgtggc atggcctttg cccgtcagtg acaacgaccc
     2881 atacagatcg accacaatat ggctcgtcgc ggttaataag ccgctacttt ccagccgatc
     2941 aataaaactt tttccggcat tcattggccc cacggtatgc gaactggagg gaccaatccc
     3001 aattttgaaa atatcgaatg cactaatcat atccacaccc tcggattgcc gttcagtgaa
     3061 gtggagcgga acgaccttgc gaccgtcccg ctcacgaggc tttacgcact acgtactgcg
     3121 atagcttcaa tttccagctt cacatctttc ggcaaacgcg caacctgcac acagctccgg
     3181 gtcgggtagg tcgcctgatg ctcatcgaag aactgcttat agacttcatt gatggtggca
     3241 aaatcattca gatcggtgat gaacacggtc atcttgatga tatcgcccac gctcagcccg
     3301 gcagcaacca cgatcgcttt gacgttttcg aggcttaaac gcgcctgatc ttgcacatca
     3361 gccgggatct caccggtctg cgggcaaacc ggaatttgcc cggaggtgaa gaccatgctg
     3421 cctaaatcaa cgccctgaac ataagggccg attgcgcctg gggcacgttg cgtttcgata
     3481 atctttttca tacatcctcc ggcgtcagag cgcctgggta aaagtacgtg aaataacatc
     3541 ctgttgctgt tcacgggtca gtgcgttgaa gcgcacggcg tagccagaga cacggattgt
     3601 caggttagga tatttttccg ggtgctcgat ggcatccagc agcatttccc gattcattac
     3661 gttgacgttg aggtgttgac cgccttcgac atccgcttcg tggtggaaat acccatccag
     3721 caggccgaca aggttggttt tacgtactgg atcttctttg cccagcgccg caggaacgat
     3781 tgagaaggtg tacgagatcc catctttggc gtaggtgaac ggcagtttcg ccaccgacgt
     3841 caatgaggcc acggcacctt tgcggtcacg accatgcatc gggttagcgc ccggcgcgaa
     3901 cggtgttccg gcgcgacgac cgtccggcgt attaccggtt ttctggccgt acaccacgtt
     3961 agaagtgata gtcagaatcg actgggtagg gacggcgttg cgataggttg gcagcgcttt
     4021 aattttcttc ataaagcgtt caaccaggtc gcaggcaatg ctgtctacgc gctcgtcgtt
     4081 gttgccgtac tgcggatatt caccgtcgat ttcaaagtcc accgccaggc cgttttcgtc
     4141 acggattggt ttcacgcggg catatttgat ggcagacagg gagtccgtcg ccaccgacag
     4201 gcccgcgatg ccgcatgcca tagtgcgata gacatcacga tcgtgcagcg ccatcagcga
     4261 agcttcgtag ctgtacttgt cgtgcatgta gtgaatgata ttcagcgcgc tgatgtactg
     4321 caccgccagc cagtccatga agtgatcgag gctgtccatc actttgtcgt aatccagcac
     4381 gtcgtccatc agcggtgctg ttttcggccc gacctgaatc ttcagcttct cgtccacccc
     4441 gccgttaatt gcgtagagca gcgttttcgc caggttagcg cgtgcaccaa agaactgcat
     4501 ttgcttacca atcaccattg ggctgacgca gcaggcaatc gcgtaatcgt cgctgttgaa
     4561 gtcagtacgc atcagatcgt cattttcata ctgcaaggaa gaggtgacga tcgacacctg
     4621 cgcggcatat tttttgaagg caatcggtaa ttcttccgac caaagaatgg tcaggttagg
     4681 ttccggtgcc ggccccatag tgtgcagggt gtgcaaatag cggaaggagt ttttggtcac
     4741 cagcgtacga ccgtccagcc ccatcccgcc gatcacttcc gtcgcccaga ttgggtcgcc
     4801 ggagaacagc gaatcaaatt ccggtgtacg caggaagcgt accatacgga tcttcatgat
     4861 gaagtgatcg atcagttcct gtgcctgctg ctcattgagt acgccagctt taaagtcgcg
     4921 ctcaatgtag atatcgagga acgatgccgt gcggcccagc gacatcgcgc cgccattttg
     4981 cgatttcact gccgccagat aagcgaagta gagccactgc accgcttcct gcgcattctg
     5041 cgccgggcga gagatatcaa agccatattt cgccgccatt tcctgaatct gcaacagcgc
     5101 atgacgatgc tctgccagct cctcacgcag acggatggtg gcttccagat cctcgccttt
     5161 ttccagacga gactggagat cggcaaattg cagttcgcgt tcacgtacca gataactgat
     5221 gccatacagc gctacgcggc gatagtcacc gataatgcgc ccacggccat agccatctgg
     5281 taaaccggtc agcacgccag atttacggca gcgcagcata tccggtgagt aaacatcaaa
     5341 tacgccctgg ttatgggttt tacgcagatc ggtaaacaga tattcaaatt cactgtccat
     5401 ttcacggcca taggcgtgga atgaactttt aatcatatta atgccaccga acgggtgtag
     5461 cgcacgtttc aacggcgcat ccgtttgcag gccaacaatt ttttccagcg gctggttaat
     5521 atatcccgca tcatgagcgg taattgtggt ggcaatattg gtatcgaaat caaccggcgc
     5581 gtgggttgcg ttttcgatac ggataccttc cattactttt tcccacaatt ccgtggtggc
     5641 aggcgtcgct tcggcgagga aagattcatc gccttcatac ggtgtatagt tatgttgaat
     5701 aaaatcgcgg acattaattt cgtttttcca gtccgtacct ttaaagccaa gccatgcgtc
     5761 ggcgtacagc ttatcgctgg tatcaatatc taccttcatg aaaaataatc tctctacaat
     5821 acttcaacta aattatgcaa attctgcggg cgcgttaact ttgcctaaat gaatggcatc
     5881 caaagcaatc attttttctt cgttagtcgg aataacggca caaatgacac gcgcattttc
     5941 actggaaaca attcgctcac cacaggagtt agagcgatta ttcatttctg tatcaatctc
     6001 tacgcctaat acagccaaat gttccatgac cagacgacga attaagcttg aattctctcc
     6061 tattccgccg gtgaatataa ttccatccag gcgacgtaat gaagctgcgt gtccggcaat
     6121 atgacgggca attcggtgaa caaaggtttt aattgccagt tgcgcgcgtt cgtgaccttc
     6181 atgccaggct ttttccagaa cacgtaaatc cgaagaaaga ccggaaatac ctaataatcc
     6241 cgactcttta ttcactacgc gttccaggtc acccaggctc tggttggttt ggctggcgac
     6301 ccaggacatc gcaccaaagt cgacatcgcc actgcgggta cccatcatca agccttccag
     6361 cggcgtcatt cccattgaag tatcaacact ctgaccgttg cgaaccgcgc agattgacgc
     6421 gccattgcca agatgcgcca caaccaggcc ggagtcatct tccgccagat tcagcagcga
     6481 atgtgcgcgc tgggaaacat agcggtgcga cgtgccgtgg aaaccataac ggcgtacacc
     6541 taactcttca taatatttcc acggcaggcc gtataaataa gcttccggag ccatcgtctg
     6601 gtggaaactg gtatcaaata ccgccacctg agttacgccc ggaaataatt gctgcgccga
     6661 ttcaatacca cttaaattgg cgtaattatg caggggtgcc agtggagaaa cgcgacggat
     6721 attatcaatg acttcatcgg taataatggc ggactcggta aaaatactgc cgccgtgagc
     6781 gatgcggtgg ccaattaagg ccacactgtc atttaaattc cgtttttcca gttcaaatgc
     6841 aattgccttc aatgcacctt cgtagctgtg gtgagccagc ggtgctggct ctcccccatt
     6901 tacggataag aatgcatttt ccgagttaat accgtcggca atacctgaca ttaatacttc
     6961 acagtcgctg gcatcgagta cggaaaactt aatcgaagac gaaccacagt taataaccaa
     7021 aacaaccgga aattcattca tctcttttct catcctgagt tacggattaa aacagtttgt
     7081 atacgatgtt caggatggtc agcagaccaa tcacggtaac aaacacgtta tccagacgac
     7141 cacggtattt cgccagagac ggcgctttac ggatggcata catcggcaac aggcacagca
     7201 gggatgcgat aatcggtgcg cccatggctt caatcaggtc aaggatgttc gggttggcgt
     7261 aggcaacaac ccaggtggag cccatgatga agatcatgct gatagtgttc agtttaccca
     7321 gcgacacttt agttttgtcg cctttataac caaacttcag gaccaggcca ttcagacctt
     7381 ccagcgttcc cagatagtga ccgaagaaag atttgaagat agccacgagt gcgatgatgg
     7441 aagccgcata ttccagtgta atcgcgaacg ttgttttggt accggtcatg gacgcaaagt
     7501 ggttagccag ataagaaagc actggaatat tctgcgcttt ggcttccgcc atgttggccg
     7561 gagacagagt aaacaggcag ctaaaggcaa agaacatcac cactgcaacc atcagcatgc
     7621 tggcacgaga aatgatttgg gaacatttac gttcggtgaa gtcgcgaccg aagtctttct
     7681 catactcttc acgcttagaa accacgaagg aagagacgat tggcgagaag ttaaaggaga
     7741 aaaccatgat ggaaatcccc agccagacag tgatcaggat accgtcatga ccggttaacg
     7801 acagcgaacc gaggtcaacc tggtcgataa ctgcagagtt ccagtaaggg atcagcgaca
     7861 aagaaatcag caccaggctg gcgataaacg gccataccag gtagctcatc actttaacca
     7921 tcagatcctt accaaaccag atgacgaaag ccatcagcag caacaggaac agcgccacaa
     7981 agccgcgatt cagcggtgca aagccgagct ggttttccca gaacgtcata aaggtattgg
     8041 taatagtaac gccataaatc cacagcagtg ggcaaatcgc gaagaagtac aggaacgtga
     8101 taaccacgcc gccagtttta ccaaaatgct cttccaccgt ttccgtaatg ttgccggaag
     8161 ggttagagcc agaaagacac agacgcgcca gcgcccggtg gcaataaaac gcgatggggt
     8221 atgccaatac caacatcaga agaatcggga tcagtccgcc aaaacctgcg cggataggga
     8281 agaacagcac cccggcgccg attgccgtac caaacaagcc taacgtccat gtggtatctg
     8341 atttacgcca ggacgattgt tttgtctggc tggatacaat gctatctgaa gtactcatat
     8401 cctatcctca acgaattaat taagcgtcaa cgaaaccggt gatttgagag acgcgagaaa
     8461 gatcgatatt gccgccggaa ataatactga cggtttttct gttttgaata tattggtcta
     8521 atttaccgct taataatgca gcacatgcca gagcgcctgc gccttcggtg acgactttat
     8581 tgcgctgaat taaggcaatc atactgtttc tgatttcgtc ttcgctgacc agcacgatgt
     8641 catcgactaa ttcacgaacg atttcgtaag ttaaattacc cgggcgggag acatcacaac
     8701 catccgccag ggtgccggta gttcggtgcg tggttatttc tccggagtgg aaagaagccg
     8761 ccatgccgtg aacgttttca gactgtacgc caataacacg aatggtcggg ttaatagatt
     8821 taattgccac cgcaatacca gcaattaaac cgccaccacc aattggcaca atcacgttat
     8881 cgacatcata gagatcttcc ataatttcca gaccaatcgt tccctggcca gcaatcactt
     8941 tcggatcatc gtaaggtggg ataaaaatac ggccttccat ttcgacaatt tcgctcactt
     9001 tagcgatagt gtcgttgaag ttatcaccat gcagaacgac ttctgcggag tagtcgcacg
     9061 ttgccgctac tttggatttt ggcgcacctt ttggcatcac cactttaccg tcgataccca
     9121 gcatcgcgca ggacagggaa accccttgcg catggttgcc cgcagaacag gccaccacgc
     9181 ctttgcgttt ttccgcatcg gtcagtgaac ttaatttatt aaatgcgcca cgaattttaa
     9241 atgaacccgt acgctgcata ttttcaaact tcaggaatat ttcacctttg caacgttcac
     9301 taaaatagtt ggagcgaggc atgcctgttt tataaattcg cccagccagt cgttgtttcg
     9361 cttcaataat gtcatcaata gcaaccggca gatcgtatgt aatatgcatt ataaaacctc
     9421 ttcgcctgaa ttaaatatgt aggtaaccgt aaccgacacc tgcaagacag acaggtggat
     9481 tatttatgtt taaataaaaa caaataacta accaacttct attaattgcc ttcgtctaca
     9541 cccattataa gatgaatact ctttggctaa ttccaccaaa accgatgctg cttttttaat
     9601 acgataattt ttcgaccata ccgcggcata ttgtgccaca ggtaatgttt cttcaaccgg
     9661 aatagtaata aattgattag aaccaaaagg tgacgtcata tcacaaggaa ttacagttaa
     9721 gaaatcagca ttgagaacaa gattataaat tgtcacgact gagtcggttt taacgatgtt
     9781 ttcaatactg atgccatttc tttgtaacgt agtaagcagt tcgctgtagt accccatatt
     9841 agtttgtggc aacacccact gttcgttctt caacgactcc agcgtggtgg tgccggtgca
     9901 tgttcgggac ttactggcta ccagcacaaa ctcggactcg aacagcggct caacatgtaa
     9961 atcctgaagc ttcatttctg cacttaacgt accaatcgca aaatccagtc gaccgtcgcg
    10021 gattgccggt aagaacgaag acagttgcgc ttcatacata gaaacctgcg ctttcgggaa
    10081 cacctctttg aacttgttga tcatccctga cataaaagta aaaccaatca atgaaggaaa
    10141 accaaatgag acttccacca ccgcctcaga agacataccg cttatctcat taaccatatt
    10201 tttcatttca cgggtaatgg attcggaacg ggagagtaac aattgaccgg caggtgttaa
    10261 tgttacaccg gtatttttcc gcaccactaa ttccacacca aaataatctt caatatcgtt
    10321 aatgatttta ctgacggccg gttgagttaa ccctaattct tttgcagccg agccgataga
    10381 gccacttcta atgacttcct gaaagactac caggtgctgc gttttcggaa gaagaatagt
    10441 gctcataacg acctacgtta attacctcat tgacggcatg aagtgtatca aaatgaaatg
    10501 aacaggatat gtgcgaccac tcacaaatta actttcaata ctttccatgg tatcgttacg
    10561 caaattaaat acctttaaat atcaacaagt taaagtataa aaatcagcat aaaccctgat
    10621 ttttgtcaaa aaaaatgacg gggataacca tattttatgg cgataacatc attcgttatg
    10681 tggttaatat aaaaaagggg ctgagaccat atttcaagca attaccggat aattaccagg
    10741 caaaatttga gttaaattta atgagcaagt tctcaaattt tattataaat aaaccatttt
    10801 cagcgataaa tactgcggca cgtcacattt tttcacgcta tttgttggag aacaaacatt
    10861 tattttatca atattttaaa atttcgaata caggtattga tcatctcgaa caattgatta
    10921 acgtcaactt tttctcttct gacaggacgt cattttgtga atgcaatcgt tttccataaa
    10981 ttcttctccc ctcataggcg acgaatagca ttttgtgttg aggatcacaa aacgaataat
    11041 tgctggtcgc cgcgataagg tcagacaaag acaacaaggg aaattttcac agagcttttg
    11101 atcgtcgtag gccacagaat gttgcttcgt ttactgtaac gccgggtaaa tgagcgtttt
    11161 ttgatagtgc gaaagaaccc tgcgaggaaa ataagcatct attattgttt gtcatgacga
    11221 caaggcaggc atcaatgaaa ggcttcccaa ttgcacatat ttttcaccct tcaatcccgc
    11281 caatgcacgc agtggttaac aatcacaata gaaatattga ttattggacg gtaaaaagaa
    11341 agtttgcaga aattgtctcc accaatgacg ttaataaaat ttacagtata agtaatgaac
    11401 tgcggagagt attatctgca attactgcat tgaatttcta tcaaggcgat gttccttctg
    11461 taatgatccg aatccaaccg gaaaatatga gtccattcat catagatatt tctacagggg
    11521 aacatgatga ttatatcata caaacattag atgtaggcac ttttgcacct tttggcgaac
    11581 aatgtacttg ctcagccgtc aataaaaaag agctggaatg tattaaggag acgatttcta
    11641 agtattgtgc aaaattcacc cgaaaagaag ccatcttaac accccttgtg cactttaata
    11701 aaaccagcat tacttcagat tgttggcaaa ttctcttttt ttcacccgat cattttaata
    11761 atgattttta ttgattattt ataaaggata aaataatgtt ccctgtttca tcaattggta
    11821 atgatattag tagtgattta gtccgtcgaa aaatgaatga cattcctgaa agcccaacag
    11881 gaaataacct cgaagcacta gcaccaggta tagaaaaact aaaacagacc tctattgaaa
    11941 tggtcacttt acttaacgcg ttacaaccag gtggaaaatg cattatcact ggtgattttc
    12001 aaaaagaatt agcgtactta caaaatgtaa ttctttataa tgactcgtct cttcgtctgg
    12061 attttttagg ttataacgcc caaattattc aacgatcgga caatacctgt gaacttacaa
    12121 ttaatgaacc attaaagaac cagaaaatat ccacaggcag tatcaatgtt aattgtccat
    12181 tgaaatatat ttacaatgaa atcaggaggt tgaacgtaat ctttagttgt ggaactggag
    12241 atatcgttga tctatcctct ctggacttac gtaatgtcga tttagattat tatgatttca
    12301 cagataaaca tatggctaat actattttaa atccttttaa attgaactca acaaatttta
    12361 ctaatgccaa catgtttcag gttaattttg ttagttcaac acaaaacgcc acaatctcct
    12421 gggattattt actaaaaata acgcctgttt taacaagcat tagcgatatg tattctgaag
    12481 aaaaaattaa gtttgtcgaa agttgtttaa atgagcttgg agatattacc gaagaacaat
    12541 taaaaactat gagatttgca attataaaat ctataccaag agcaactctt acagataaat
    12601 tagaaaatga attaacaaaa gaaatatata aaagctcatc gaaaatcaat aattgcttga
    12661 acagaattaa attaccagag atgatagatt tctcatcaga aaaaatacat gattatatcg
    12721 acataatcat tgaagattat gaaaatataa aagaaaatgc ttatctggta attccccaaa
    12781 ttaattatac tatggattta aacatagaag actctagctc agaagagtta ctttcagata
    12841 acaccctcga gaaagacgaa aattctccgg acaatggttt tgaggtcggg gaatataaca
    12901 catatgaagc atataactca gagaatcaat attttaccag agaggactat acgtataatt
    12961 acgacctttt aaatgcaata tagacttaat aacttcttac aaagtctatt attaataccg
    13021 tagatattta ttcatcttac cctgtgcacc aaaagcaaaa actcactgac gcgggtttga
    13081 aactgatggc aaccgcaaaa atgcctgatg cgctacgctt atcaggccta cgccatctct
    13141 gcaatatatt gaatttgcgt gcttttgtag gcaggataag gcgttcacgc cgcatccggc
    13201 atgaataaag cgcactcaac aatctgaaac ccgccggaac ggtttattac gtacatcagg
    13261 tgaaactgac cgataagccg ctttcttttg ggtatagcgt cgtggacagt cattcatctt
    13321 tccgctcctc caaaagcaaa aacccgccgg tttacgctgg cgggttttcg ttaaatcagg
    13381 tgaaactgac cgataagccg ggttctgtcg tggacagtca ttcatctagg ccagcaatcg
    13441 ctcactggct caagcagcct acccgggttc agtacgggcc gtaccttatg aacccctatt
    13501 tggccttgct ccgggtggag tttaccgtgc cacggactgt taccagccgc gcggtgcgct
    13561 cttaccgcac cctttcaccc ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc
    13621 tctgttgcac tggtcgtggg tttccccccc aggcgttacc tggcaccctg ccctatggag
    13681 cccggacttt cctcccctcc gcccgtctcc cccgaagagg acgacgacga agcggcgact
    13741 gtctggtcag cttcggcgcg cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc
    13801 gcattccaat cgccagtgtc gcggcgatat tacgtgaagc acggcagata ttgtcataag
    13861 ccccgcggaa tgcttcgtcc aacgtaccta tgctggtcaa tacgctgaag accgcatcga
    13921 tgccatgctg atgtacaacg ccaacatcat cggtcaggct acccgcaatg ccaatcaccg
    13981 gtttatggta cttcttcgcc acgtttgcaa cgccaatcgg taccttgccg tgaatactct
    14041 ggctgtcaat acgcccttca ccggtgatca ccagcgtaca atcgtgaata tgttcctcca
    14101 gattcagcgc cgtagtgacg atttcaatac cacttttcag ttccgcacca agaaacgcca
    14161 tcagcgccgc acccataccg cccgcagctc ctgcgccggg gacatctttc acatcaacat
    14221 gcagcgcttt tttaatgacc tcggcatagt gagagaggtt attgtccagc tcaacaatca
    14281 tcgcttcact ggctcccttt tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca
    14341 gcggattggt gacatcacaa gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc
    14401 cggaaatatc aatatcattc agagtattaa gactaccgcc gccaaaacca atttcattgc
    14461 cgttggcgtc gcataatttc gcccccagcg cctgtaccat gcctgcgccg ccatcatttg
    14521 tagcgctacc gccaatgccg ataataatgt ttgtcgcgcc gctctccagc gcctgcagga
    14581 ttaactcgcc tgtgccgcgt gaagtggtca cgagtggatc gcgtttttcc gcaggtacca
    14641 gctccagccc actggccgcc gccatttcaa taaacgcggt tttgccatcg ccggagatcc
    14701 cccaactggc attcactttc tcgcccagcg gccctgtaac ccaggcgtga cgttcagaac
    14761 cctgggtggc tgcaatcatc gcttccaccg ttccttcgcc accgtcggca accggaatag
    14821 aaacgtactg tgcatcagga aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct
    14881 cgctggcaga taaactttct ttataagagt ctggggcgat tacgattttc atacctatgc
    14941 ctgttaccac atgacgccgg agggcgtttc tcttattcgg cctggattcc aggcccggat
    15001 tgcaatacgc catccgggca cgacgtcatt aacgagtaac ttcgactttc gccagttttt
    15061 cgtagtagca cgccagggcg ctatgatccg ccgttcctaa accatctgct cgcagtgcct
    15121 gcatcatctc cataaccgca gctgtgagcg gcagttgcgc gccgacgccg tgggaagtat
    15181 ccagcgcatt tgccagatcc ttaatatgca gatcaatacg gaagcccggc ttgaagttgc
    15241 ggtccatcac catcggcgct ttggcatcca gcacggtact gcccgccagt ccaccgcgaa
    15301 ttgcctgata caccaggtcc gggttaacgc ccgctttggt tgccagcgtt agcgcttctg
    15361 acatcgcggc aatattcagc gccacaatga cctgatttgc cagtttggtg acgttacctg
    15421 caccgatttc cccggtatgc accacggaac ccgccatcgc tttcatcaaa tcatagtatt
    15481 tgtcgaaaat agccttgtcg ccgcccacca tcactgacag cgtaccgtcg atggctttcg
    15541 gttcaccgcc gctcaccgga gcatccagca tatcaatgcc tttcgctttc agcgcttcgc
    15601 tgatttcacg gcttgccagc ggtgcgatag aactcatatc gatcaatacc gtacctggct
    15661 tcgcgccttc aatgatgcca ttctcaccca gcgccacctc tttcacatga ggggagtttg
    15721 gcagcatggt tatgatgacg tcgcactgtt cagcgatcgc tttagccgta gacgctgttt
    15781 ctgcacctgc agcaatcacg tcagcaatag cttctgggtt acggtcagca accaccagcg
    15841 agtaacctgc tttcagaagg tttttactca ttggtttacc cataatcccc aggccaataa
    15901 aaccaacttt catagtcata tcaatcatct ctcttgttgc ggtggtggtt attttttaaa
    15961 ggtatcagcc agtttctgag tggcagagcg gaagacgcca agatcgctgc cgacagccac
    16021 aaacgtcgcg ccccattcca gataacgacg cgcatcggct tcgaccggcg cgagaatacc
    16081 gctgggtttg ccgtgcgcac tggcacggtt aaaaatgtgc tgaattgctt tttgtacatc
    16141 cgggtgcgag gcattgccga gatggcctaa tgccgcagcc agatcgctgg ggccgacgaa
    16201 gataccgtct acaccttcgg tagcggcaat ggcatcgacg ttatctacgc cctgctgact
    16261 ttctatctgg accagaatgg tgatgttctt gttcgactga gcgaaataat ccgccacggt
    16321 gccaaacata ttggcgcggt gagaaacgga gacgccgcga atgccttccg gtgggtaacg
    16381 ggttgatgcc accgcctgct ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt
    16441 atagaaaccg atatccagaa gacgcttaat aattaccggc tcgttggtcg gcactcgcac
    16501 tactggcgcg ctggcgctgc ctttcagggc cattaactgc ggaataaacg tggagatatc
    16561 gtttggcgca tgttcgccat ccagcaccag ccagtcaaac ccagccaaac caagaacttc
    16621 agtgctaatc gggttagaga gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag
    16681 tgcggctttg aatttattcg ggaaaacatc gttattcatc gcttatacct ttgcttattt
    16741 ctgcaattcc atacgtttaa tgtcgccaac tacgaagagg tagcagacca tcgccatcag
    16801 cgctgaacat cccacgaaaa ccagtgctgc attgaaggag tgcagttcac ttaccaggta
    16861 gccaatcacc agtggagtga caatggaggc aacattgcca aagacgttaa agacgccgcc
    16921 acagaggcca acaatctctt tcggcgcggt gtcagaaatc accggccagc ccagagcacc
    16981 aaatcctttg ccaaaaaaag ccagcgccat tagcatgacc accagcgtgg tgttgttggt
    17041 gtagttacat aagatgatgg tggaagccag caacattccc agcacaatcg gtagcttacg
    17101 tgccagggtc agggataaac cgcgtttgat cagataatcc gagaagacac ctcccagcac
    17161 gccgcccgca aaaccacaca gtgccggaat cgaggcgacc agacccactt tcagaatcga
    17221 catgcctttt tcctgcacca gataaatcgg gaaccaggtg aggaagaacc aggtgatggt
    17281 gttgataaaa tattgtccga aaaatacgcc caacatcatg cggttagaga gcaattgctt
    17341 gatgtaatgc agtttgggac cgcttgctgc cgcactgccc ggctttttgt ggtccatatc
    17401 gaccaccgcg ccattttcag agataaactt cagctcttcc gcagacatac gtgggtgatc
    17461 tgtcgggtta tgaatcaatt tgatccacag cgccgtcagc acaaaaccaa tcacccccat
    17521 aacggtaaag acgtgctccc agccccaggc gaaagtcagc cagccaagca gaggcgaaaa
    17581 gagcgccagc gagaaatatt gcgccgagtt aaagatggcg gaggcagtac cacgttcttt
    17641 cgtcgggaac caggcggcga caatacgggc gttcgccggg aatgatggcg cttccgagaa
    17701 gccgagcata aagcgcataa agaacatgga gatccctgcc caggccagcg ggaacatatc
    17761 aacaaagcct tgcaggaaag tgaatagcga ccagaaaaag aggctatagg tataaacttt
    17821 tttcgagcca aacttatcaa gcagccagcc gccggggatt tgcatcagca agtaggccca
    17881 gccaaaagca gaaaagatgt aacccatcga aaccgcactt aactgcaact cttttgccac
    17941 ttcggtacca gcaatagaca gcgttgcacg atcggcgtag ttaacggcgg taacaataaa
    18001 aataatcagt aatattaaat agcgggtatg cacgcctttc tttttttcgt caacggtgtc
    18061 cagaatcatt ttatttacct cgggtactta tgctgatttt tattattatg gggaaggtgt
    18121 tatttatgag tttcatttat gccgtaacgg caatgaactc gggaattagt ataagcagcg
    18181 cgagaataat aatcattgtg caaatgctaa tttaattaat gctatttaaa tattattttg
    18241 agcatatgca cataaggttg cgcgctaaag cacagatttg cgctttacct tgccgggcgg
    18301 cactgcaatc cctgaaatga ttgacattga tcacatttct gcgtttaaac tcctgacatt
    18361 cttatttcac ccaatgaagt catttatttt taaatgagac caggtcctca ttttaataac
    18421 ccctggctgg agaatatagt acaatggcca acatcgaaat cagacaagaa acgccaactg
    18481 cgttttatat aaaagttcac gacacagata atgtggcaat tattgttaat gataatggcc
    18541 tgaaagcagg aacgcgtttt ccggatggac tggaattaat tgaacatatt ccccaggggc
    18601 ataaagtcgc attgctggac attccggcta atggtgaaat tattcgttat ggtgaagtga
    18661 ttggttacgc cgtgcgtgca atcccacgcg gaagctggat cgacgaatca atggttgtac
    18721 taccggaagc gccgccgtta cacacgctgc cactggcaac caaagtcccg gcccccttac
    18781 cgccgctgga aggatacacc tttgagggct atcgcaatgc cgatggcagc gtgggcacca
    18841 aaaacctgct cggcatcacc accagcgtcc actgtgtggc aggcgtggtg gactatgtag
    18901 taaaaatcat tgaacgcgat ctgctaccga aatacccgaa cgtcgatggc gtggtggggc
    18961 tgaatcattt gtacggctgt ggcgtggcga ttaacgcacc ggcggcagtt gtgcctattc
    19021 gtaccatcca caatatttcg ctgaacccta actttggcgg cgaagtaatg gtgattggtc
    19081 tgggttgtga aaagttgcag cctgagcgcc tgctggtcgg cacggatgat gtgcaagcta
    19141 ttccagtaga aagcgccagc attgtcagtt tgcaggatga aaagcatgtc ggttttcagt
    19201 ccatggtcga ggatattttg caggtcgccg aacgccatct acaaaaactg aatcaacgtc
    19261 agcgagaaac ctgtccggct tcagaactgg ttgtcggcat gcagtgcggt ggcagcgatg
    19321 ccttttctgg cgtaacggca aacccggcgg ttggctatgc gtctgatcta ctggtgcgct
    19381 gcggcgcaac ggtgatgttc tcagaagtca cggaagtgcg tgacgcgatc catctgctga
    19441 caccacgcgc agtgaacgaa gaggtcggca aacggctgct ggaggagatg gagtggtacg
    19501 ataactatct caatatggga aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga
    19561 aaggcggtct ggcaaacgtg gtagagaagg cactcggctc cattgctaaa tcgggtaaaa
    19621 gcgcaattgt tgaagtgctg tcgcccggtc aacgcccgac taaacgcgga ttaatttacg
    19681 ccgcgacgcc agccagcgat tttgtctgtg gcacgcaaca ggtggcttcg ggtatcaccg
    19741 tgcaagtgtt tacgaccggt cgtggtacgc cgtacggcct gatggcggta cccgtcatta
    19801 aaatggcgac ccgcaccgag ctggcgaacc gctggtttga tttaatggat attaacgcgg
    19861 gcactatcgc caccggcgaa gaaaccattg aagaggtggg ctggaagttg ttccacttta
    19921 ttctcgacgt cgccagcggg aagaagaaaa ccttctcgga tcaatggggg ctgcataacc
    19981 agctggcagt gtttaacccg gcaccggtga cctgatttct tttggaacgt ccctcgcaaa
    20041 acatggcctt agtgccatgt ttttattgtt taaagccccc acgtccatta ataatgcatt
    20101 tgcattacct tagttcaagc ttataatttg agcagaaaac aggctgtaaa aggacagtga
    20161 atcatgcccg ctaatgctcg ctctcacgct gtactgacca ctgaatcaaa ggtcacgata
    20221 cgcggacaaa caactatccc cgcgccagtg cgtgaggcct taaaactgaa gccaggccag
    20281 gacagcattc attacgaaat tctgcctggt gggcaagtct ttatgtgccg actgggagat
    20341 gaacaggagg atcatactat gaatgcattt ttgcgttttc tggatgcaga tatccagaac
    20401 aacccgcaaa aaactcgtcc attcaacatt caacaaggaa agaaacttgt cgctggcatg
    20461 gacgtcaaca ttgatgatga gattggcgac gacgaataat ggattttcca caaagggtta
    20521 atggttgggc gctatatgct catccctgtt ttcaggaaac ctacgacgct ttagttgccg
    20581 aagtcgagac attaaaggga aaagatcctg aaaattatca gagaaaagcc gccacaaagt
    20641 tattggcggt agtccataaa gtgattgagg agcatatcac ggtcaatcca tcatcaccgg
    20701 cattccgtca tggcaagtcg ttaggctctg ggaaaaataa agactggtca cgggtaaaat
    20761 ttggtgctgg tcgttatcgt ctcttctttc gttatagtga aaaagagaaa gtcatcattc
    20821 tgggatggat gaacgatgaa aacactctgc gcacctacgg taaaaaaaca gatgcctata
    20881 ccgtattcag caaaatgtta aaaagaggac atcctcctgc cgactgggaa agcctcaccc
    20941 aagagacaga agaaaaccat tgatggtgtt cacaatgccc gccgaacgta ttcgtgttga
    21001 ccagcgggca ccctttctca ctccccgacc agaatcactt caaccccagc ctttcgcagt
    21061 ccttccaggc tatccgcagg aatgccttca tcaacaatga tcatgtcgat acgttgagta
    21121 tcaatgatct tatgtaaact ggaacgattg aacttactgg aatcggtgac gacgatgatc
    21181 cgttccgcaa cttcgcacat ccgacggttt aaacgggctt catcttcatt atgtgtgctg
    21241 acgccgcgct ccagatcgat cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt
    21301 tgcagcgatt gctcagcctg atcgccgtaa aaagattgcg actgacggcg caaatgcccg
    21361 ccggtcatca gcagctcaac gccttccgct tccagcaacg cattagccac gttcataccg
    21421 ttggtcatcg caattacgtc agtgtgcttg cgcatcagac gagcaatctc aaaagtggtg
    21481 gtcccggaat cgaggatcac ccgatgacct ggctgaatca actcaacggc agctttcgca
    21541 acgctgcgtt tcatcgcggt gttcagcgcg cttttatctt ccactgatgg ctcgactggc
    21601 ggcgtcgtgc tatcgcagat caacgcgcca ccataggcac gcacagcgat cccctgcttt
    21661 tccagaaacg ccagatcatt gcggatcgtc acagtagata cgccatacaa tgccgacaga
    21721 tcgttaacct gcacactccc ttgctgtcgc agacgctgaa tgatctgttc tcgtcgctcg
    21781 ctggtgccag tcactcgctt ctcacctgaa gcgtcggtat tactcatagt aagtcctttc
    21841 gtaaaacttt cgtttcattt cgttttgcct attaacgcct ttctattaag caaatgcaag
    21901 cccaccttgc ccattgacgc aagctactct cgtttcagtg actttcatta tgtttctttt
    21961 gtgaatcaga tcagaaaact attatctttc gttttatttt tatctcacca tgacgcagta
    22021 tcaactgaaa caaaacgaaa gattaatatc gcagtaatct gaactggaga ggaaagtgaa
    22081 acatctgaca gaaatggtga gacagcacaa agcgggcaaa acaaatggaa tttatgccgt
    22141 ttgttccgca catccgctgg tgctggaggc tgcaatccgc tacgccagtg caaaccaaac
    22201 gccgttactg attgaagcaa cctccaatca ggtagaccag ttcggcggtt ataccggaat
    22261 gacgcccgcc gattttcgcg gctttgtttg tcagctcgcc gaatcgttga atttcccgca
    22321 ggatgcgttg attctgggtg gtgaccatct ggggccaaac cgctggcaaa acctgccggc
    22381 cgctcaggca atggccaatg ccgatgattt gattaaaagc tacgttgcgg caggattcaa
    22441 aaaaatccac cttgattgca gcatgtcctg tcaggacgat ccgattccct taactgatga
    22501 catcgtggct gaacgcgccg cccgtctggc gaaagtggcg gaagaaacct gtcttgaaca
    22561 ctttggcgaa gccgatctgg agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc
    22621 gcatgaaacc ttaagcgagc tggcggtcac cacgccggat gccgcccgcg ccacgctgga
    22681 agcccatcgt cacgcctttg aaaagcaagg tttgaatgcc atctggccac gcatcattgc
    22741 cctggtggtt caacccggcg tcgaattcga tcacaccaac gttattgatt atcagcccgc
    22801 caaagcgagc gccttaagcc agatggtcga aaactacgaa acgctgattt tcgaagcgca
    22861 ctctaccgat tatcaaacgc cgcaatcgct gcgccagctg gtgattgacc actttgccat
    22921 tctgaaagtt ggcccagcgc tgaccttcgc cctgcgtgaa gctctgttct ctctggcggc
    22981 gattgaagaa gaactggtgc cagcgaaagc ctgttctggt ctgcgtcagg tgctggaaga
    23041 cgtgatgctc gaccgcccgg aatactggca aagccactac cacggtgacg gcaacgcgcg
    23101 tcgtctggcg cgtggttata gctactcgga tcgcgtgcgc tattactggc cggacagcca
    23161 gattgatgac gctttcgctc atctggttcg taatctggcg gattcaccaa ttccgctacc
    23221 gctcatcagc cagtatctgc cgctgcaata cgtgaaagtt cgctccggcg agctgcaacc
    23281 gacgccacgg gaactcatta tcaaccatat tcaggacatc ctggcgcagt accacacagc
    23341 ctgtgaaggc caataagcaa aataaagagg aacacgctat gccaaatatt gttttaagcc
    23401 ggattgatga gcgtttgatt cacggtcagg tcggcgttca gtgggtcgga tttgcggggg
    23461 caaatctggt gctggtagcc aacgatgagg ttgccgaaga tccggtacaa caaaacctga
    23521 tggaaatggt actggcagaa gggatcgccg tacgtttctg gacgctgcaa aaagttatcg
    23581 acaacatcca tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa acacctgccg
    23641 atttcctgac gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc
    23701 actacgccaa tggcaagcaa caaatcgcca aaacggtttc tgtggatgcg ggtgatatcg
    23761 cagcatttaa cgacctgaaa accgctgggg tggaatgctt cgttcagggc gtcccgacag
    23821 agcctgctgt ggacctcttt aaattacttt gagggattca tcatggaaat cagtctgttg
    23881 caggcattcg cgttgggcat tatcgccttt atcgctggcc tggatatgtt taacggtcta
    23941 acccatatgc accgcccggt ggtcctcggc ccgttggtcg ggctggtact tggcgatctg
    24001 cataccggta ttttaaccgg cggtacgctg gaactggtgt ggatggggct ggccccgctg
    24061 gcgggcgcac agccgcctaa cgtgattatc ggtactatcg tcggcacggc gtttgccatt
    24121 actactggcg tgaaacccga tgtcgcggtc ggtgtcgccg tacctttcgc tgtcgcagta
    24181 cagatgggga ttaccttcct gttctcggtg atgtctggcg tgatgtctcg ctgcgaccgg
    24241 atggcggaga acgccgatac tcgcggcatt gaacgcgtga actatctggc actgctggca
    24301 ctcggcactt tctattttct ctgcgctttc ctgcctatct acttcggcgc ggaacatgca
    24361 aaaaccatca ttgatgtcct gccgcaacga ttaatcgacg gcctcggcgt cgcgggcggc
    24421 atcatgccag caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacatc
    24481 ccctacttca tcctgggctt tgttgccgca gcctggctca agttaccggt gctggctatt
    24541 gctgccgctg cgctggcgat ggcgctgatc gacctgctgc gtaaatctcc tgaaccgact
    24601 caacctgcgg cacagaaaga ggaatttgaa gatggcatct aatcaaacca ccctgccgaa
    24661 cgtcagtgaa aacgaagaaa cattgctgac tggcgtcaat gaaaacgtgt atgaagatca
    24721 gagcattggc gcggagctga cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgct
    24781 gttacaagcg tcttttaact acgaacgtat gcaggcttct ggctggttgt acggtctgct
    24841 gcctgctctg aaaaagatcc acactaataa acgcgacctg gcgcgcgcca tgaaggggca
    24901 tatgggtttc ttcaataccc atccgtttct ggtgacattt gttatcggca ttatccttgc
    24961 gatggagcgt tctaagcagg acgttaacag tattcagagc accaaaattg ccgtcggtgc
    25021 gccgctcggc gggattggcg atgcaatgtt ctggctaacg ctactgccga tttgtggcgg
    25081 gataggcgca agccttgcgc tacaaggctc tattcttggc gcagtcgtat ttattgtgct
    25141 gttcaacgtg gtacacctgg ggctgcgttt tggtctggcg cattatgctt accgcatggg
    25201 cgtggcggcg attccactca ttaaagcgaa taccaaaaaa gtcggccatg ctgcgtctat
    25261 cgttgggatg acggtaatcg gcgcgctggt ggcaacctat gttcgtttaa gcaccacgct
    25321 ggaaatcaca gcgggcgacg cagtggttaa gttacagact gatgttattg acaaactgat
    25381 gcctgccttc ttaccgctgg tctacacctt gaccatgttc tggctggtac gccgcggctg
    25441 gagtccgctg cgtctgattg cagttaccgt ggttctcggc atcgtcggta aattctgcca
    25501 tttcctttaa atacaatgag gtattagatg ttaagtatta ttttgacagg gcatggcggg
    25561 tttgccagcg gcatggaaaa agcaatgaag caaattctcg gtgagcaatc gcagtttatc
    25621 gccatcgatt ttccggaaac ctcaagcacc gcgctgctta catcgcagct tgaagaggcc
    25681 atcgcccaac tggattgtga agacggcatt gtttttctga cggatttgct gggcggcacg
    25741 ccgtttcgcg tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt gatcaccggc
    25801 accaatttgc aactgctact ggagatggtg ctggagcgcg aagggttaag cggtgaagaa
    25861 ttccgtgtac aggcgctgga gtgtggacac cgagggctga ccagtctggt ggacgagtta
    25921 ggccgctgcc atgaagaatg tccggtcgag gaaggaatat gacacacgtt ctgcgcgcca
    25981 gaaggctgct gactgaagag ggatggctcg atgaccatca gttgcgtatt gctgacggtg
    26041 tcatcgcagc aatcgaaccg attccagtgg gcgtgactga acgcgatgcg gaactgctct
    26101 gccccgctta catcgacacc catgtacacg gtggtgcggg cgttgatgta atggatgacg
    26161 cgccggatgt actcgacaag ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt
    26221 tgccgactac cgtaaccgcg ccgcttaaca gcattcatgc ggcgctgaaa cgtattgctc
    26281 aacgttgcca acgcggcgga cctggtgcgc aagtgctggg gagttatctc gaaggaccgt
    26341 acttcacgcc gcagaataaa ggcgcgcatc cgccggaatt gtttcgcgag cttgaaattg
    26401 ccgagctgga tcaattgatt gccgtttctc agcacacctt acgcgtggta gcgctggcac
    26461 cggaaaaaga gggggcattg caggccatcc gccatcttaa acagcaaaat gtacgagtga
    26521 tgctggggca tagcgcggcg acctggcaac aaactcgcgc cgcgtttgat gctggtgccg
    26581 acggcctggt gcattgctat aacgggatga caggtttaca tcaccgcgaa ccgggaatgg
    26641 ttggcgcggg attaacggac aagcgcgcct ggctggaact gatagccgat ggtcatcatg
    26701 tgcatccggc ggcgatgtcg ctgtgttgtt gctgtgcaaa agagagaatc gtgctgatca
    26761 ccgacgcgat gcaggcagcc gggatgccgg atggtcgcta tacgttatgt ggcgaagaag
    26821 tgcagatgca cggtggcgtt gtccgtaccg cgtccggtgg gctggcgggc agtacgctgt
    26881 ctgttgatgc ggcagtgcgc aacatggtcg agttgacggg cgtaacgcct gcggaagcca
    26941 ttcatatggc atcgctgcat ccggcgcgaa tgctgggtgt tgatggtgtt ctgggatcgc
    27001 ttaaaccggg caaacgcgcc agcgtcgttg cgctggatag cgggctacat gtgcaacaaa
    27061 tctggattca gggtcaatta gcttcgtttt gatggtttgc tccattattg ggccttcact
    27121 tcccccgtaa ggcctttctt tttctttctt tttgatctgt gcagcggtgt cggatgcgac
    27181 gctaacgcgt cttatgcgac ctacggtacg ccgcatccgg caatcggtga acatagcgac
    27241 gtcattacgc aattcttaat cttcccttgt cttttctttc aacgatcaca gatttcgttt
    27301 tatttccttt ttctccattg aactttcagt ttcttttcta tagattttaa tcaacgaaag
    27361 acatcaccaa gtgaaatgaa acgaaaggca agtgaaagcg acaacgcccg acgtcaagtt
    27421 catcagacta aggattgagt tatgccagaa aattacaccc ctgctgccgc cgcaaccggt
    27481 acatggactg aagaagagat ccgccatcag cctcgcgcat ggatccgttc actcaccaac
    27541 atcgacgcgc tacgttctgc gctcaataac ttccttgaac cgttactgcg caaagagaat
    27601 ctgcggatca tcctgaccgg agccggaacc tcggcattta tcggtgacat catcgcgccg
    27661 tggctcgcca gccataccgg taaaaacttc agcgccgtac cgaccaccga tctggtcacc
    27721 aatccgatgg actacctgaa cccagctcat ccgctgctgt tgatctcctt cggtcgatcc
    27781 ggcaacagcc cggaaagcgt cgctgccgtg gaactggcaa atcaatttgt accagaatgc
    27841 tatcacctgc cgatcacctg caacgaagcg ggcgctcttt accaaaacgc gatcaacagc
    27901 gataacgcgt ttgccctgct gatgcccgca gaaacgcacg atcgcggctt tgcgatgacc
    27961 agcagcatta ccaccatgat ggccagctgc ctcgcggttt tcgcacctga gacgatcaac
    28021 agccaaacct tccgcgacgt ggcggatcgt tgccaggcga tcctgacctc actgggcgat
    28081 ttcagcgaag gtgtgtttgg ttacgcaccg tggaaacgga tcgtttatct cggcagcggt
    28141 ggcttacagg gcgcagcacg cgagtcggcg ctgaaagtgc tggaactgac ggcgggtaaa
    28201 ctggcggcct tttatgattc tccaaccgga ttccgtcatg gaccaaaatc gctggtcgat
    28261 aacgaaacgc tggtggtggt atttgtctcc agccaccctt acacccgtca gtatgatctt
    28321 gatctgctgg ctgaactccg ccgtgacaac caggcaatgc gcgtaatcgc catcgccgcg
    28381 gaaagcaccg acatcgtcgc tgccggtcca catattatcc tgccgccgtc acgtcacttt
    28441 atcgacgttg agcaggcatt ttgcttcctg atgtacgccc agacgtttgc actgatgcag
    28501 tcgctgcaca tgggcaatac gccggatacc ccatcagcca gtggcaccgt taaccgcgtg
    28561 gtgcaaggcg taatcattca tccgtggcag gcataagagg atcgcattat gagcattatc
    28621 tccactaaat atctgttaca ggacgcccag gccaatggct acgcggtgcc tgcttttaac
    28681 attcataacg ccgagacgat ccaggcgatc ctcgaagtgt gcagtgaaat gcgatcgccg
    28741 gtgatcctcg ccggaacgcc ggggaccttt aaacatatcg cgctggaaga gatctacgcc
    28801 ctgtgcagcg cctattccac aacctacaac atgccactgg cgctgcatct cgaccaccac
    28861 gaatcgctgg atgatattcg ccgtaaagtc cacgcaggtg tgcgcagtgc aatgatcgac
    28921 ggcagccact tcccgtttgc cgagaacgtg aagctggtga aatcggttgt tgacttctgc
    28981 cactcacagg attgcagcgt ggaagcagaa ctgggccgcc tgggcggtgt tgaagatgac
    29041 atgagcgttg acgccgaaag tgcattcctg accgatccac aagaagctaa acgctttgtc
    29101 gaactgactg gcgtcgacag cctggcggta gcgattggta cggcgcacgg cttatacagc
    29161 aaaacgccga agattgattt ccagcggctg gcggaaattc gtgaagtggt ggatgttcct
    29221 ctggtgctac atggtgccag cgatgttccg gatgaatttg tccgtcgcac tattgaactt
    29281 ggcgtcacaa aagtgaacgt tgccacagaa ttaaaaatag ccttcgctgg cgcggttaaa
    29341 gcctggtttg cggaaaatcc gcagggtaat gatcctcgtt attatatgcg cgtcggaatg
    29401 gatgcgatga aagaagttgt cagaaataaa attaatgtct gtggttcagc gaatcgaatt
    29461 tcagcataat cattagtttt ttatgattta tcccaatgta cttcccgatt aatatcgggg
    29521 agtgccttaa tggaaaagga gatgactaaa ccttaataaa tacatcagta caatatcgca
    29581 acaataatat attaaaaaaa attatattat tcaactttat ggtgaggatt acacaatgag
    29641 cagtccaaat attctcttaa cccgtattga taaccgtctg gttcacggtc aggttggcgt
    29701 gacctggaca tccaccatcg gtgcgaatct gctggtagtc gtggatgatg ttgtcgctaa
    29761 cgatgatatt caacagaaat taatgggtat taccgcagaa acctacggct ttggcattcg
    29821 tttctttact atcgaaaaaa ccattaacgt catcggcaaa gctgcaccgc atcagaagat
    29881 cttcctgatt tgccgtacgc cacaaacggt acgtaaattg gtagaaggtg gcattgacct
    29941 gaaagatatc aacgtcggca atatgcattt ctcggaaggg aaaaagcaaa tcagcagtaa
    30001 agtttatgtc gatgaccagg atctcgcgga cttacgtttt attaaacaac gtggcgtgaa
    30061 tgttttcatt caggacgtcc ctggcgatca aaaagaacaa atccctgact aaatctaaaa
    30121 tcgccttaat attggtttga ggtaataaaa atgcatgaaa taaccctact tcagggatta
    30181 tccctggcgg cgttagtttt cgttctgggg attgattttt ggctggaagc cttattttta
    30241 ttccgcccga taatcgtttg taccctaact ggcgctattc tcggtgatat tcagactggc
    30301 ttaattaccg gtggtctgac agagttggct ttcgccggat taacccctgc aggtggtgtt
    30361 cagccgccca acccgattat ggcgggtctg atgaccaccg tcattgcatg gtctacgggc
    30421 gttgatgcca aaacagcaat tggtcttggc ctgccgttta gtttgttaat gcagtacgtc
    30481 attctgttct tctattccgc tttctcatta tttatgacca aagccgataa atgcgcgaaa
    30541 gaggcggata cggcagcgtt ttcccggctt aactggacaa cgatgctcat cgtcgcttca
    30601 gcgtatgcgg tgattgcttt cctctgtact tacctggcac aaggggcgat gcaggcgctg
    30661 gtgaaagcga tgcccgcctg gttgacccac ggctttgaag tggctggcgg tattctgcct
    30721 gccgttggtt ttggcttgct gctgcgcgta atgttcaaag cgcaatatat cccttacctg
    30781 atcgccggtt tcctgtttgt ttgctacatc caggtcagca acctgttgcc ggttgccgta
    30841 ctgggcgcag gctttgcggt gtatgagttt ttcaatgcga aatcccggca gcaagcgcaa
    30901 ccgcagcccg ttgccagtaa aaatgaagaa gaggactaca gcaatgggat ctgaaatcag
    30961 taaaaaagat atcacccgtc tgggctttcg ttcgtcgctg ctgcaagcga gctttaacta
    31021 cgaaaggatg caggcgggcg gttttacctg ggcgatgttg ccgatcctga aaaagattta
    31081 taaggacgac aaaccgggct taagcgcggc gatgaaagat aacctcgaat ttattaatac
    31141 ccacccgaat ctggtcggat tcctgatggg gttattaatt tcgatggaag aaaaaggaga
    31201 aaaccgcgac accattaaag gcctcaaagt ggcactgttt ggcccaatcg ccgggattgg
    31261 cgatgcgatt ttctggttta ctttgttgcc gattatggcg ggaatttgct catcatttgc
    31321 cagccaggga aacctgctgg ggccgattct atttttcgcc gtttacctgc ttatcttttt
    31381 cctgcgcgtc ggctggaccc acgtcggtta ttcagtcggc gtgaaggcga tcgataaagt
    31441 gcgagagaac tcgcagatga ttgctcgctc ggcaaccatc ctcgggatca cggtaatcgg
    31501 cgggctgatc gcttcgtatg tgcatattaa cgtggtgaca tcgtttgcca tcgacagtac
    31561 ccacagcgtc gcgctgcaac aggatttctt cgataaagtc ttcccgaaca ttttaccgat
    31621 ggcctacacc ctgctgatgt attacttcct gcgggtgaaa aaagcgcatc cggtgctgtt
    31681 aatcggcgtg acttttgtgc tctctattgt ttgttccgca ttcggcattt tgtaaatgga
    31741 acgaggcact gcttctggcg gtgcctcttt acttaaggaa tttcatcctg tgcaaaccct
    31801 tcagcaagtt gaaaactata cggcgttaag tgaacgagcc agcgaatatt tattggctgt
    31861 gatccgtagc aaaccggatg ccgtgatttg cctggcgacc ggagccacgc cattactgac
    31921 gtatcattat ctggtagaaa aaatccacca gcagcaggtt gatatcagcc agctcacctt
    31981 cgtgaagctc gacgaatggg tggatctgcc attaacgatg cctggcacct gcgaaacttt
    32041 cctacaacag catatcgtgc agccgctggg gctgcgtgaa gaccagctca tcagctttcg
    32101 ctccgaagag ataaatgaga cagagtgcga acgggtaacg aacctgattg cgcgcaaagg
    32161 cggtctggat ttatgcgttc taggattggg gaaaaacggt catcttgggc tgaacgaacc
    32221 gggagaaagc ctacaaccgg cctgccatat cagtcaactt gatgccagaa cacagcaaca
    32281 tgagatgtta aaaaccacgg gtcgccccgt gactcgtggg atcaccttag gcctgaagga
    32341 tattctcaat gcccgcgagg ttttgttatt ggtgactggc gaaggaaagc aggatgcgac
    32401 agaacgtttt ctcacggcta aagtctccac cgctatcccg gcttcgtttt tatggctgca
    32461 cagtaacttt atttgtttaa ttaataccta accagatgaa aatttgctca aaatttttaa
    32521 attaacttat tcaacagtca cgcattatat taaataacat ttgactgggt tgaacataac
    32581 gccaatagca aaaggagtta tgttcaacaa tttaatttta gatatacata taacttgttc
    32641 tattaataaa tcacctcgta ttctgactcg gcccctggcg cagcaaagtc aggcatttat
    32701 actcttttta acacatagaa aattcatatc aatataataa aaacatagaa tatttgcata
    32761 taaatataat cttaaagttt agtctattta atgttcaatg aaatatttct acctgtataa
    32821 tctttaaaga tgttgaacat atattcacat taaatatgat tatgtacttg ttacaaggat
    32881 aaggttataa atgaataaag ttacaaaaac agctattgtg ggcttacttg cgcttttcgc
    32941 aggtaatgcc gctgcaaccg atggtgaaat agtttttgat ggcgagattt taaaatctgc
    33001 ttgtgaaatc aatgactctg ataagaaaat tgaagttgct cttggtcact ataatgccga
    33061 acagtttcgt tctgttggcg atcgtagccc aaaaattcca ttcactattc ctttagttaa
    33121 ctgcccagtg actgggtggg agcacgataa cggcaacgtt gaagcgtctt tccgtttatg
    33181 gctggaaacg cgggataatg gcaccgtccc taacttccct aatctggcaa aagtgggctc
    33241 ttttgctggc acagcggcaa caggtgtggg tatccgtatt gacgatgcgg aaagcggaaa
    33301 ccttatgcca ctgaatgcta tggggaatga taacacggtt tatcagatcc cagcggactc
    33361 caatggtatt gtcaatgttg acctcatcgc ttactacgta tcaaccgttg aagcatcaga
    33421 aatcacccca ggggaagcag acgctgtcgt taacgtaacg ctggattacc gttaatacgt
    33481 tacggcgtta tctgaccttt cagataacgc ccttttcctt cctctttctc gttgtatcag
    33541 gttgaaaaat aactatgtca aaacgaacat tcgcggtgat aataaccttg ttgtgtagct
    33601 tctgtatcgg ccaggcgctt gcagggggta tcgttttaca gcgaacgcga gtgatctatg
    33661 atgccagccg caaagaggct gcgttacctg tcgcaaacaa aggcgcagaa acgccttatt
    33721 tactgcaatc atgggtagat aatatagatg gtacaagccg tgccccattt attataaccc
    33781 caccgctatt tcgtcttgaa gctggcgatg actcatcact gcgaattatt aaaacagctg
    33841 ataacctgcc tgaaaataaa gagtcgctgt tctacattaa tgttcgtgcc attccagcaa
    33901 agaaaaaatc agatgatgtt aatgctaacg agttgacgct ggtatttaaa acacggatca
    33961 aaatgtttta tcgccccgca cacctgaagg gacgggtaaa cgatgcgtgg acatcactgg
    34021 aatttaaacg tagtgaccat tcactcaata tatataaccc aactgaatat tacgtcgtat
    34081 ttgccggact ggcagtcgat aaaaccgatc tcacaagcaa aattgaatat atcgcgcccg
    34141 gagaacataa acagttacca cttcctgcat ctggcggaaa gaacgtgaaa tgggctgcaa
    34201 tcaatgatta tggcggcagt tccgggacag aaactcgtcc actgcaataa caaatataaa
    34261 aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac gcacaccgaa
    34321 acagttggcg ctcatcatca aacgctgttt gccgatggtg ctcactggca gcggcatgct
    34381 ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg aaactacaaa
    34441 aagtggtatg caaacaactg atctgtcacg tttttcaaaa aaatacgcac aactaccagg
    34501 aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa aaattacatt
    34561 taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac tacgtgagct
    34621 gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata gcgtgatcaa
    34681 ctcgcttgaa caaatcattc ccggtacagc tgctgaattt gatttcaatc atcagcgact
    34741 taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg tctccccttc
    34801 tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag gttctgataa
    34861 ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa atggtgctaa
    34921 ttttggcccc tggcgattac gtaactattc tacgtggaca cgcaacgatc aggcgtcaag
    34981 ctggaacact atcagtagtt atttacaacg tgatatcaag gcgttgaagt ctcagttgct
    35041 tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta ctggcgtgca
    35101 actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc caacggtacg
    35161 cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg tgatctatca
    35221 aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt ccaacagcgg
    35281 cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta tccagcctta
    35341 ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga ccgctggacg
    35401 ctatcgcgct gatgcaaaca gtgatagcaa ggaacccgaa tttgctgaag ccacggcaat
    35461 atatggtttg aataatactt ttacgctgta tggcggcctg ctcggttctg aagattatta
    35521 tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt cgatggatat
    35581 caacagagct gacacccaat tcgataaccg gcactctttt catggctatc aatggcgtac
    35641 gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgttagct actatcgcta
    35701 taccaacgat ggctatttta gttttgatga agccaatacc cgtaattggg actataacag
    35761 tcgccaaaaa agtgaaattc aattcaacat cagccagaca atattt
//
LOCUS       DAEVZA010000035        35552 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEVZA010000035 DAEVZA010000000
VERSION     DAEVZA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.035"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..112
                     /locus_tag="LAF92_001816"
     CDS             <1..112
                     /locus_tag="LAF92_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="HyaD; endopeptidase involved in the cleavage of the
                     C-terminus end of HyaB (the large subunit of hydrogenase
                     1); Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBI9790835.1"
                     /translation="PQPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            109..507
                     /gene="hyaE"
                     /locus_tag="LAF92_001817"
     CDS             109..507
                     /gene="hyaE"
                     /locus_tag="LAF92_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBI9790836.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            504..1361
                     /locus_tag="LAF92_001818"
     CDS             504..1361
                     /locus_tag="LAF92_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBI9790837.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQMSQRQPPLGEVLAVDLLNLHADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            1495..3039
                     /gene="appC"
                     /locus_tag="LAF92_001819"
     CDS             1495..3039
                     /gene="appC"
                     /locus_tag="LAF92_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBI9790838.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            3051..4187
                     /gene="appB"
                     /locus_tag="LAF92_001820"
     CDS             3051..4187
                     /gene="appB"
                     /locus_tag="LAF92_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBI9790839.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            4200..4292
                     /gene="cbdX"
                     /locus_tag="LAF92_001821"
     CDS             4200..4292
                     /gene="cbdX"
                     /locus_tag="LAF92_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBI9790840.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            4372..5670
                     /gene="appA"
                     /locus_tag="LAF92_001822"
     CDS             4372..5670
                     /gene="appA"
                     /locus_tag="LAF92_001822"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBI9790841.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(5785..7965)
                     /gene="etk"
                     /locus_tag="LAF92_001823"
     CDS             complement(5785..7965)
                     /gene="etk"
                     /locus_tag="LAF92_001823"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBI9790842.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(7985..8431)
                     /gene="etp"
                     /locus_tag="LAF92_001824"
                     /gene_synonym="yccY"
     CDS             complement(7985..8431)
                     /gene="etp"
                     /locus_tag="LAF92_001824"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBI9790843.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLDGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(8419..9558)
                     /locus_tag="LAF92_001825"
     CDS             complement(8419..9558)
                     /locus_tag="LAF92_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBI9790844.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(9604..11700)
                     /locus_tag="LAF92_001826"
     CDS             complement(9604..11700)
                     /locus_tag="LAF92_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBI9790845.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(11700..12446)
                     /locus_tag="LAF92_001827"
     CDS             complement(11700..12446)
                     /locus_tag="LAF92_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBI9790846.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(12443..13087)
                     /gene="gfcB"
                     /locus_tag="LAF92_001828"
     CDS             complement(12443..13087)
                     /gene="gfcB"
                     /locus_tag="LAF92_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HBI9790847.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(13194..13499)
                     /locus_tag="LAF92_001829"
     CDS             complement(13194..13499)
                     /locus_tag="LAF92_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HBI9790848.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(13901..14113)
                     /gene="cspH"
                     /locus_tag="LAF92_001830"
     CDS             complement(13901..14113)
                     /gene="cspH"
                     /locus_tag="LAF92_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBI9790849.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            14399..14611
                     /gene="cspG"
                     /locus_tag="LAF92_001831"
     CDS             14399..14611
                     /gene="cspG"
                     /locus_tag="LAF92_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBI9790850.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            14622..14810
                     /locus_tag="LAF92_001832"
     CDS             14622..14810
                     /locus_tag="LAF92_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBI9790851.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQICTDHAPLDFTK"
     gene            14785..15015
                     /gene="ymcE"
                     /locus_tag="LAF92_001833"
     CDS             14785..15015
                     /gene="ymcE"
                     /locus_tag="LAF92_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBI9790852.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            15005..15178
                     /locus_tag="LAF92_001834"
     CDS             15005..15178
                     /locus_tag="LAF92_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBI9790853.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(15226..16299)
                     /locus_tag="LAF92_001835"
     CDS             complement(15226..16299)
                     /locus_tag="LAF92_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBI9790854.1"
                     /translation="MAENKRIRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(16371..19115)
                     /gene="torS"
                     /locus_tag="LAF92_001836"
     CDS             complement(16371..19115)
                     /gene="torS"
                     /locus_tag="LAF92_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBI9790855.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            19198..20226
                     /gene="torT"
                     /locus_tag="LAF92_001837"
     CDS             19198..20226
                     /gene="torT"
                     /locus_tag="LAF92_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBI9790856.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKTKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(20199..20891)
                     /gene="torR"
                     /locus_tag="LAF92_001838"
     CDS             complement(20199..20891)
                     /gene="torR"
                     /locus_tag="LAF92_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBI9790857.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            21021..22193
                     /gene="torC"
                     /locus_tag="LAF92_001839"
     CDS             21021..22193
                     /gene="torC"
                     /locus_tag="LAF92_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBI9790858.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            22193..24739
                     /gene="torA"
                     /locus_tag="LAF92_001840"
     CDS             22193..24739
                     /gene="torA"
                     /locus_tag="LAF92_001840"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBI9790859.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVICIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            24736..25335
                     /gene="torD"
                     /locus_tag="LAF92_001841"
     CDS             24736..25335
                     /gene="torD"
                     /locus_tag="LAF92_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBI9790860.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(25428..25733)
                     /gene="cbpM"
                     /locus_tag="LAF92_001842"
     CDS             complement(25428..25733)
                     /gene="cbpM"
                     /locus_tag="LAF92_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBI9790861.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(25733..26653)
                     /gene="cbpA"
                     /locus_tag="LAF92_001843"
     CDS             complement(25733..26653)
                     /gene="cbpA"
                     /locus_tag="LAF92_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBI9790862.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            26913..28166
                     /locus_tag="LAF92_001844"
     CDS             26913..28166
                     /locus_tag="LAF92_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBI9790863.1"
                     /translation="MGNIHGISCTANNYLKQAWNDIKNEYKKNQTYSITLFENTLVCF
                     MRLYKEIRRQQAEDYIPCLECDSLEKEFEEIQNDNDLSLFMKTLRTNDKQIYSGVSGG
                     ITYTIQYVEDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENITACDDVFSEYCLDD
                     ENISVQPERINTPGISDLDSGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAILV
                     LKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQSD
                     NKIQGEGSLMPWNFLFFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEKRIN
                     EVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPPE
                     LFAQAYELNNKERNAQIDFLTRYAI"
     gene            28461..29702
                     /gene="agp"
                     /locus_tag="LAF92_001845"
     CDS             28461..29702
                     /gene="agp"
                     /locus_tag="LAF92_001845"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBI9790864.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(29740..29967)
                     /locus_tag="LAF92_001846"
     CDS             complement(29740..29967)
                     /locus_tag="LAF92_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBI9790865.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(29988..30584)
                     /gene="wrbA"
                     /locus_tag="LAF92_001847"
     CDS             complement(29988..30584)
                     /gene="wrbA"
                     /locus_tag="LAF92_001847"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBI9790866.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVSETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            30957..31130
                     /locus_tag="LAF92_001848"
     CDS             30957..31130
                     /locus_tag="LAF92_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBI9790867.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(31213..32541)
                     /gene="rutG"
                     /locus_tag="LAF92_001849"
     CDS             complement(31213..32541)
                     /gene="rutG"
                     /locus_tag="LAF92_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBI9790868.1"
                     /translation="MAMFGFPHWQLKSTSIESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(32562..33056)
                     /gene="rutF"
                     /locus_tag="LAF92_001850"
     CDS             complement(32562..33056)
                     /gene="rutF"
                     /locus_tag="LAF92_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBI9790869.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(33067..33657)
                     /locus_tag="LAF92_001851"
     CDS             complement(33067..33657)
                     /locus_tag="LAF92_001851"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBI9790870.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            complement(33667..34467)
                     /gene="rutD"
                     /locus_tag="LAF92_001852"
     CDS             complement(33667..34467)
                     /gene="rutD"
                     /locus_tag="LAF92_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBI9790871.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(34475..34861)
                     /gene="rutC"
                     /locus_tag="LAF92_001853"
     CDS             complement(34475..34861)
                     /gene="rutC"
                     /locus_tag="LAF92_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBI9790872.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(34873..>35552)
                     /gene="rutB"
                     /locus_tag="LAF92_001854"
     CDS             complement(34873..>35552)
                     /gene="rutB"
                     /locus_tag="LAF92_001854"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBI9790873.1"
                     /translation="ARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPV
                     IANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGK
                     LLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNV
                     CVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSP
                     TSFARIA"
BASE COUNT         8646 a         9042 c         9199 g         8665 t
ORIGIN      
        1 gccgcaaccg gctaatgaat cgcgctgtct caattatgac tgtctgtcga tggaaaatta
       61 cgaaggcgtt cgtttgcgcc agtacaggat gacactggag gagcagggat gagcaacgac
      121 acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg gctggacgcc ggtcagtgaa
      181 tcccgtcttg acgactggct tacgcaagcg ccagacggcg tggtgttatt aagcagtgac
      241 ccgaaacgta cgccagaggt cagcgataat ccggtaatga ttggcgaatt actgcgcgag
      301 tttcccgact atacatggca ggtggcgatt gctgaccttg agcagagcga agccatcggc
      361 gatcgctttg gcgtctttcg ctttcccgcc acattagtgt ttaccggcgg aaactatcgc
      421 ggtgtgctga atggtattca cccgtgggcg gaactgataa acctgatgcg cgggcttgtc
      481 gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt ccatctgctg gggccaggaa
      541 cgcaaccgaa cgatgacagt ttcagcatga atccactgcc gatcacctgt caggtgaatg
      601 atgaaccgag tatggcggcc ctggaacaat gtgctcacag cccgcaggtg attgcgctgt
      661 taaacgagtt acaacatcaa atgagtcaac gccagccgcc gttgggcgag gtgctggcag
      721 tcgatctgtt aaatctccac gccgacgatc gtcactttat caataccctt ctcggggaag
      781 gggaagtgtc agtgcgcatt cagcaggctg acgacagtga aagtgaaata caggaggcga
      841 tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga aaagttgctg gaggacaaac
      901 tggaagctgg ctgtgcgccg ctggcgttgt ggcaggcggc aacgcaaaat ctcttgccga
      961 cagattcgct gttaccgccg cccattgatg gcctgatgaa tggcctaccg ttggcgcatg
     1021 agttactggc acatgtacgt aaccccgacg cgcagccgca cagcattaat ctgacgcaat
     1081 tacccatcag cgaggctgat cggctttttc tctcacgtct ctgtgggccg ggaaatattc
     1141 agattcgtac cattggctat ggcgagagct atatcaacgc cacggggtta cgccatgtct
     1201 ggcatttacg ctgtacggac accttaaaag gccctttact ggaaagttat gaaatctgcc
     1261 cgataccgga agtggtgctg gcagcgccag aagatttggt cgactctgcg cagcggctta
     1321 gcgaggtatg tcagtggctg gcggaagctg caccgacgta aaaagacggt aagtatcgct
     1381 ttcagtctta tgaatatcgc aatcggcgaa tacctctggt cgtagagttt caggataaag
     1441 agggagatct accattatcg ggttattttt ctctcttcgc ctacaggagt gcgcatgtgg
     1501 gatgtcattg atttatcgcg ctggcagttt gctctgaccg cgctgtatca ctttttattt
     1561 gtacccctta ccctggggct gatttttttg ctggctatca tggaaaccat ttacgtggtc
     1621 accggcaaaa caatctaccg cgatatgacg cgcttctggg gtaagctctt cggtatcaat
     1681 tttgctcttg gcgtggctac cggcctgacc atggagtttc agtttggtac taactggtca
     1741 ttctattcca actatgtggg cgatattttt ggcgcaccac tggcgatgga agcattaatg
     1801 gccttcttcc tcgaatccac ctttgtcggg ctgttcttct tcggctggca acggctgaat
     1861 aaataccagc acctgctggt gacgtggctg gtggcgttcg gttcaaatct ctctgcgttg
     1921 tggatattga atgccaacgg ttggatgcaa tacccgaccg gtgcgcattt tgatatcgac
     1981 accctgcgta tggagatgac cagcttcagc gagctggtct ttaacccggt cagccaggtg
     2041 aaatttgtgc acaccgtaat ggcgggctac gtgaccgggg ccatgtttat tatggcgatc
     2101 agcgcctggt atttactgcg cggacgggag cgcgatgtcg cattacgttc gtttgccatc
     2161 ggttccgtct tcggtactct ggcgattatc ggtaccctgc aactcggaga cagttctgcg
     2221 tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga tggaagggga gtggcaaacg
     2281 gaacctgcac ctgcaccgtt ccatgtggtt gcctggccgg aacaggatca agagcgtaac
     2341 gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg ccactcactc attagataaa
     2401 cccgtgccgg gtctgaagaa tttgatggct gaaacctacc cacgcttgca acgcggacgt
     2461 atggcctggc tgttaatgca ggaaatatcg caaggcaatc gtgagccgca tgtgttgcag
     2521 gcattccggg aactggaagg tgacctgggc tacggcatgt tgctctcccg ctatgcgccg
     2581 gatatgaatc atgtcacagc cgcacagtac caggcggcga tgcgtggcgc gatacctcag
     2641 gttgcgccgg tattctggag tttccgcatc atggtgggct gtggttccct gctgctactg
     2701 gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa tcgaccagca tcgctgggtg
     2761 ctgaaaatgg cgctctggag tttgccgttg ccgtggattg cgattgaagc cgggtggttt
     2821 atgaccgagt ttggtcgtca gccgtgggcg atacaggaca tcttaccgac atactccgcg
     2881 cactccgctt taaccacagg acaactggct ttctcactga tcatgatcgt agggctttac
     2941 accctgttct taatcgccga agtctacctg atgcagaaat atgcccgtct ggggccgagc
     3001 gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa ggagaaaatc atgtttgatt
     3061 atgaaacatt gcgcttcatc tggtggctgc tgattggcgt gatcctggtg gtctttatga
     3121 tctccgacgg atttgacatg gggatcggct gtctgctgcc gctggtggcg cgtaatgatg
     3181 atgaacgccg gatagtgata aacagcgttg gtgcacactg ggaaggcaac caggtctggt
     3241 tgatcctcgc tggtggggca ttatttgccg cctggcccag agtgtatgca gcggcgtttt
     3301 ccggctttta tgtggcgatg atcctggtgc tgtgctcgct gttcttccgt ccgctggcct
     3361 ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa aatgtgggat gccggtctgg
     3421 tcatcggcag tctggtgccg ccggtagtct tcggtatcgc cttcggcaac ttgttgctcg
     3481 gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta tctcggcagc ttctggcaac
     3541 tgctgacgcc attcccttta ttgtgcggat tgctcagcct tgggatggtg attttgcaag
     3601 gtggcgtctg gttacaactg aaaactgttg gtgtgattca tctgcgttca cagctggcga
     3661 ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct ggcgggttac tggctgtggg
     3721 tcggtattga tggctttgta ctgctcgccc aggatgctaa cggtccttcc aatccgttaa
     3781 tgaaactggt ggcagtgcta cctggtgcct ggatgaataa tttcgtcgaa tcgcccgttt
     3841 tgtggatctt cccgctgctg gggttctttt gcccattgct gacggtgatg gcgatttatc
     3901 gtggtcgccc ggggtgggga tttttgatgg cgtcattgat gcaatttggc gtgattttca
     3961 cggcaggcat cacgctgttc cccttcgtca tgccgtcaag cgtgagtccg atctccagcc
     4021 tgacgttgtg ggacagcact tccagccagc tgacgctgag tattatgttg gtaatcgtgc
     4081 tgatattttt gcccattgtg ttgctctaca ctctctggag ctactacaaa atgtgggggc
     4141 gcatgacaac agaaactctc cgccgtaacg aaaacgagtt gtactaagga gcaggaacaa
     4201 tgtggtattt actttggttc gtcggcattt tgttgatgtg ttcgctctcc acccttgtgt
     4261 tggtatggct ggacccgcgt ctgaaaagtt aacgaacgta ggcctgatgc ggcgcattag
     4321 catcgcatca ggcaatcaat aatgtcagat atgaaaagcg gaaacatatc gatgaaagcg
     4381 atcttaatcc catttttatc tcttctgatt ccgttaaccc cgcaatctgc attcgctcag
     4441 agtgagccgg agctgaagct ggaaagtgtg gtgattgtca gtcgtcatgg tgtgcgtgct
     4501 ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc aacctggccg
     4561 gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcctatct cggacattac
     4621 caacgccagc gtctggtagc cgacggattg ctggcgaaaa agggctgccc gcagtctggt
     4681 caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga agccttcgcc
     4741 gccgggctgg cacctgactg tgcaataacc gtacataccc aggcagatac gtccagtccc
     4801 gatccgttat ttaatcctct aaaaactggc gtttgccaac tggataatgc gaacgtgact
     4861 gacgcgatcc tcagcagggc aggagggtca attgctgact ttaccgggca tcggcaaacg
     4921 gcgtttcgcg aactggaacg ggtgcttaat tttccgcaat caaacttgtg ccttaaacgt
     4981 gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact caaggtgagc
     5041 gccgacaatg tctcattaac cggtgcggta agcctcgcat caatgctgac ggagatattt
     5101 ctcctgcaac aagcacaggg aatgccggag ccggggtggg gaaggatcac cgattcacac
     5161 cagtggaaca ccttgctaag tttgcataac gcgcaatttt atttgctaca acgcacgcca
     5221 gaggttgccc gcagccgcgc caccccgtta ttagatttga tcaagacagc gttgacgccc
     5281 catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtgct gtttatcgcc
     5341 ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg gacgcttccc
     5401 ggtcagccgg ataacacgcc gccaggtggt gaactggtgt ttgaacgctg gcgtcggcta
     5461 agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca gcagatgcgt
     5521 gataaaacgc cgctgtcatt aaatacgccg cccggagagg tgaaactgac cctggcagga
     5581 tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag gttttacgca aatcgtgaat
     5641 gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca gttacctgaa
     5701 tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa aaggcgttca
     5761 cgacgcatcc ggccactttc agttttactc tttctcggag taactataac cgtaatagtt
     5821 atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga tagcgccttt
     5881 aatattgacg cctgcctgtt ccagacgctg cattgacaaa ctcacctctt tggcggtgtt
     5941 caagccaaaa cgcgcaacca gcaggctggt gccaacagaa cgccccacga ccgcggcatc
     6001 actcaccgcc agcatcggcg gcgtatcgac aatcaccaga tcgtaatggt cgttcgccca
     6061 ttccagtaat tgacgcatcc gatcgcgcat cagcagttca gacgggttag gtggcacctg
     6121 accgcgagta atcacatcaa agcctccttt gccaaaatgc tggatcactt tgttgagctc
     6181 atctttacct gccagatatt ccgacaagcc atgttcatta ctcacggtaa acaggttatg
     6241 cgaataacca cggcgtaagt cggcatcaat aaataacact ttttgatcgg actgggcgat
     6301 caccgctgcc agagttgaac tgacaaacgt tttaccactg tctggcgtcg caccggtgat
     6361 catcagaatg ttattctccg tctccatcat agcgaaatgc agactggttc gtagcgcacg
     6421 tacggcttcc acagcagaat ccgccgggtt atccaccgcc aggaagggga tatttttagt
     6481 acgatggcgc tgctgattag aaaataaatt tttcttacgc agacgggtgc gtttatccag
     6541 ccactcggac attgggatag tggcataaac gctgatgccg tgctcttcca gttgttccgg
     6601 ggcttctaca ccacgacgca acatcgcacg cgccagcacg gcacccacag aaataaacag
     6661 gccaagaata aaaccaagca ccacattcaa cgcttttttc ggtttcactg gctgcggctg
     6721 agtgactgcc gggtcgataa tccgcacgtt accaatggca ctggatttcg aaatactcaa
     6781 ctcctgctgg cggttaagta attgcagata taccgcacgg cccgcttcta cgtcacgact
     6841 taaacgcaac acttcctgtt gggtggaagg cattgccgat acccgcttat tcaggcgttt
     6901 gcgttcttgc tccagcgtct ggcgtttttc cagcagcgca cgataagttg ggtgatcttt
     6961 cttatacagc tgggagatct ctgcctcgcg gaaggtcagc tcattgagtt gattatcaac
     7021 gttcacaatc tgctcaagaa cggctttggc ttccaggtta aggtcaaccg aatcgcgctg
     7081 ctggcgataa acgttgagtt tttcttccgc ctggtccagc tcgctgcgca cttcaggtaa
     7141 ctggcgctgt aagaattcaa ggctttgtga atcctgcgcc gcctggcgag cgatattctg
     7201 ttgcaaatag ttgttagcga tgctgttcag aatacgagta atcaactggg gatcatcacc
     7261 agtcatggta agttccagca tcccgctttc tttactgcgt tcgctaacgg taaaggtttc
     7321 ctgcaatgcg ttaatcgctt ccagttcggt acgctggctc aggacaaact gtgttcctgg
     7381 tttggcctta atgtccgcga tagtcagcgc aacgccatct ttttccagac gctggccgac
     7441 cataccattg acggtgaact cttcaccttc cagtgtatag tggccgtttt ccccaaccgt
     7501 gagtgtcagt tgctgatcct gaccattcag ttgtggaata tgcatccagc tgatcgccag
     7561 ctcacctggt ttttctttgg ttaatctcgc ccagccgcga cccacaatcg gaaaatactt
     7621 ctgctcaact atgtcgcgca gattcagttc agcaatggtt ttaccgagaa tcatgcgcga
     7681 ttgcagcagt tggatctccg gtgcagactc gggcgatgag ttagggatca tatcgctcag
     7741 gccgctgaga atggcgttgc cctgtttttg ctcaacctgg accagagtat ctgcctgata
     7801 aattggtgtg cttaacagcg agtaagcgac agcgatcagc gtgaataacg cggtcacgct
     7861 gataataaac ttacggtgat cccataactc gccgaccaga cgaagcagat cgatctcatt
     7921 ttcctgagtg ctgcctggtg gcgtattcat atttttagtt gtcatcgtat tttataaact
     7981 caaattaccg gctgaggcgc ttcgcccatt cctgactggc gcgctccaac ataccgtaga
     8041 catgttcaaa tgcgtcctga cttttacgat agggatccgg gatctctttc tgttccagcc
     8101 attgcccaaa cagcattgtc ttcccgcgga cctcgggagc aattgcggta acctgagcaa
     8161 tatgttccga ctccattgcc agaatcagat cgtaatttcg tgccatctca gcagtgagct
     8221 tacgtccggc atgtccatca agagatacgc catgattagc ggcaacatct gccgctgtcg
     8281 catctgcagg gtgttttacc agaccatgaa cacctgccga tttgactttt acgcccggca
     8341 gacgtttacg cagcaagcgc tcgccaatgg gcgaacggca aatgttgccg gtacaaacca
     8401 ccaggattga gttaaatttt agttgggcca tgtttgaatc caccgtgaag tctctgtcag
     8461 gtcatgcacc ccggaaatcg tcggtaccag cagagaaatc acacggttcc aacgagccag
     8521 cggcgcggta gtgacataaa caatgtcgta aggttcaagc tggaattccg ttccgagtat
     8581 cattgccgat gcgtccttcg cattcagctg ataaatattg gcgattttgc cattctgctt
     8641 gttttgcgtt gcacgaataa caaaaatccc cgtcgcatca gcgacatcct gattcatacc
     8701 ctcagcgtta cccagcgctt ccgccagggt cataccgctg cggtccattt tcagcgtgct
     8761 ctgtttaccc acttcaccca tcacgaagac tttcagatca tcgttgcgcg gaataaataa
     8821 aatatcgccc gggtgcagca gtttgttctg gcgcaagtcg ccacgctgca tcagcgcata
     8881 gaggttcact ttggtcttta cgccattctg ggtgagcacc acgttgcgcc agtctgcatc
     8941 tgcggtcaga ccgcccgcgg cgttaatcgc atccatgatg gtcagaggaa tattggtaat
     9001 cggctgctga cccgatttgc tgacttcgcc ggtgacgtac gctttttgcg agcggaacgc
     9061 cgcgacgctg acatcgactt gcgggctttc aatgactgaa tccagacgcg ctgtaatctc
     9121 gttgcgtacc tgggtaagtg ttttcccggc cactttcagg cgaccaatat aggggtagaa
     9181 aatcgcacca tcggcgttaa cccagttgcc ggtatcactg gcgctacggt attgccctgc
     9241 gggggtggtt agttccgggt gatcccagac cgtaaccatc aacacatccc caatgccgat
     9301 acgatattca tagtttgcga tttgctgctc cagctccggg ttagcctgcg acataatcgg
     9361 tttggcgcgc aactgatcaa tcaatcctgg cgtgacggga tagatattga ccattttgtc
     9421 caggtcatac tgattgtcag gtagttcaat gacatccttg ttactggtcg ataaattctg
     9481 accaggaaca agggtacatg ctgtcaaaga cagaacagcc aatgtcagta ccgaaaactt
     9541 aaaaatgttc tttttcattg gtttatattt atcagtcaat gtaatgttat gaattacgtc
     9601 aggttattgt ccaaccggta cttctcgctc tgccgtcatt ggatagagct ggtatttgcg
     9661 tgacagcatt tgtccaccat cacgcgtcaa tggtgtccac gaaaccaccg cgcggttgcg
     9721 gttaggtcca atggtcatca agtcgaatgg aatcgagata taaaaacctt tactaaagcc
     9781 gccttcgccg tagtcatctt tagatacgtt actgattgcc gcccataccc ctaccgccac
     9841 gccgctgtca aagcgtttgg cgacgtcgat cgttgcgcct ttatcttttg ccagatattg
     9901 cccaacgcta agtttcatca gtacgccatt gagcgtcggc gggttccagt aagccgtcac
     9961 gaaacctgtt ggcgtggaat aatcggtgaa acgcatcatg ttgtcccagt cacgctgctt
    10021 cacgtagtta acgtccacac ccagcgccca gctggcatct agcgggcgat aaagcagctc
    10081 ggaaccgaca ccggcataca tcgtttccag ataaccgcca tacacctggc catagaaacc
    10141 attgcctaag tcagcaaaat agttcgcctg caagttgttg agataaacgt cattgcgaac
    10201 gtagtcacgg atatgcgtgc gcacgcgggg cagggtagag tccgcgggca acagcgaaga
    10261 cttaaacttg tcgtagttgt tgtaaatatt ggtgaaaata ccgccatcaa gcagcaggtg
    10321 gtcggtaaac cagtagcggg cactggacat cagccccagc tggaacatat agaaatcttc
    10381 cgggccgccc agcgactgtg aaagtgttgg gttgaaagag tagctaaagc gatcttcacg
    10441 aatacggtag ccccgaccaa aggcagaaag atcttctgct tcaacacgtt gttgttgaag
    10501 ttgctctgat tgaccaggcg ctgtaccagc cagctgtttg cgcaggctgg ctacgtcggt
    10561 ttcggtagtc accatcgcca tatgctcgcg cttttgcgtc acgctaatct tctcaacgcc
    10621 ttgcggcagg ttattcacca gaatccggtt ggcacgatcg accgcttcgc gagaatcacg
    10681 gtatttgtat tgttgaccag acatatacag cgtcttatcg cgcagctgaa tttccggtgc
    10741 ttcaaaacct gcgttgtact tcagcgcggt aagttggttc gctactgtgg tgtactgcaa
    10801 tccttcagat tcaggcgcag gttgatatgc cggttttggc gtatcgcgca gggcagggcg
    10861 cagatcgttg aaattggtcc gtaacgtgaa gccaaacatc aacgtgttac cgcgttcata
    10921 actcaggttg agatctgccc agctggcagc gcgataaact gcgccgacgt tgaaatggct
    10981 tgcctgaggc agtttgccag cgaaatcatt ctggtaattg ttgccatcgt attcgagttt
    11041 cagacgcagg ggattccacg gcgtttgata ctcaatgccg ccaaagatgg aagccgggcc
    11101 acgaaagata tcgctaaagc tgatatcgcc cgcatcgtga gactctgcgc gatgacaata
    11161 tttatcgctc acccggcaaa acgggttggt aatattgccc gcattaccgg cgtagcccca
    11221 tgccatcccg agggtgaaat caaatggccc cgcttgcttg ctggccacca gatactcacc
    11281 gtcaaacaga cccgtaccag caatatcacg tttaccaaac gccacttgcg gtagccagta
    11341 accttcttcc cacaggcgaa gtttaaaatc gaatgatttg tctttgtatg actgatcgcc
    11401 gctgaaatct tcccactggc tatatttgcg tgtgcgcaca tccgtataac gaatggtgcc
    11461 ttccagccat gggaacagcg ccacggaggt ggagtagaac cggtattgat cgttatcccg
    11521 gtagttgacg ctgaattcac cttccggtgc gatgcgcgca tttggcatct gcaacaatcc
    11581 tgtgccgcca aagtctgatt gcgacgaacc cagcggatcc gggtaggtta atacttcagc
    11641 atgacaggca ctggagacgg caatggccag gcagcttaaa agataagaat tcttcttcat
    11701 cagtcaggaa cacgttgcgt taaaacagac acaatctggt catttaaatc agcatatttt
    11761 tcgggcagta catgcgcaga aaagcccaac caaagctggc tgccaggcgg tggttccaca
    11821 tgtcttttat tccacagggc aacaggggcg acgacagtct caccctcagg cgtaatcacc
    11881 ataacgttat tcttgtccgc gcctgcaagg cgggggtgat cttgcagata atcggtaacg
    11941 ctacggcctg cctgccaggg caattgcccg gccccagaaa ccgcacccag cagagtaata
    12001 gtcaccggac gctgtacggt atagagcgta tagtccccga ccaacggggg attgctgttc
    12061 tcatccactc gtacaaaatc agggtccagt ttgaccggta agcgaccagt gatattgaga
    12121 ttgagtaact gctggcgcac ggatttaatc gttgccgcca catcgtcgtc agcttctgct
    12181 tcccaggaag caagctgtgc catgacatgt tggtagtctt taagcgcttt agctttcgcc
    12241 gcagagtcgg tcagcaaggc accaggccac cagaggcgat cgcgcagttg cggttgtgtc
    12301 accaactgga caacattttc catggggcca acggaaagtg tctgttgttc gccaggcaga
    12361 taaatagtca ccgttccttg cgcaaaggca tggggtgtca ttacgtaaag tacgctggca
    12421 atgaaatacg actgtaattt attcatggtg cgggtttaag aaacgtcatt tctacgggaa
    12481 taacccctgc gcctaacatt tgccgacttt gacgaacctg gccagtagcc gaatcgaccc
    12541 agaatgtgtt ttgccactgt ttttctggcc gcgtggaagt cacctcttcc tgccagacat
    12601 tacacagcac attgcgaccc gcaatattca gcgtctctaa tccagcaaag gaaaaagtag
    12661 aactcagggt cgcagagcga aaatggttgt cttcactcca gagaatatcg cgcgtccagc
    12721 ggctgccatc ttttattgcc agcgcgttac gcagagggtc ctgcccggaa tttgtcactt
    12781 ccagcagatt attattaagt ttgacggttt ttaaaagacg tccattgtgg gtaaccagca
    12841 tggcgttatc ctgggataac catttgcttt gttcttgttc tatatatccc agtaccacaa
    12901 agattcgttg cccttcatta aggcgtaaat acatcgtgga ataaggcaac gcctggatct
    12961 gctgatccgc tacggtgata tcctgattat cgaaaaggct ggcgcgaaat gtatcgacca
    13021 tactttgctg gctgtgcgta catcccgcaa gaaatagtgc gaaaatcgat aaaataagag
    13081 ggcgcacctt taatccttga aaggttaatt aaaaaaatgc aaatctctaa agcccggtac
    13141 gaatatcgca accgggtatc atttaacttt aatttcgacg cgttaaagca gtattagcgt
    13201 gtagtggacg tggtggttga ggtgttatga gaaccgccat cgccactgtc atttgctgca
    13261 acaactgcca acgtcgccac accaacagcc gttgccacac cgacaactgt cgccgtgccg
    13321 acagcgccag cagccacgcc accagtggta gtagcagtcg tggttgcgcc ggtagtggtc
    13381 gttgtggtgc cagtagtacc cgtcgttgcc tcggtcccgt ttgttgcttc aggggcagca
    13441 aacgcagctg gcgtcgtcaa catgaaggcc ataaggattg cagaaagctt gtgtttcata
    13501 acttttcctt tattcatcgc atggacaata cgtacgcttg agatcaggat tgtctacttt
    13561 gagtataaca tccttgtgaa atctcccttt cctcataccg cttgcactat atactgcggg
    13621 taggaaaggc gcgcagaggg aaatataaga ttgttaacta tccccctctg gaaatacgac
    13681 ttgtgagcat aagataaatc ttagcaaaat acaaattaaa gataaggaaa ttgttacgaa
    13741 agctattaat tattgttggt aatatagttt caagtggtac tataactggc tgttaaatgg
    13801 attagtttct ttgtggaaaa gtaactgatg ttataatgta tgatgttgac tattgcacta
    13861 ttccaataaa accagtcagc tttaaacaag cagcgtcata ttaagagaga taaacatttg
    13921 ccgctgttgg tcctcgcagg ccatttacgc ggcaaaattc cacacgtaat cctggtataa
    13981 gcacttctgc gtcgcgggga gtgaatgcgg aaatatggac ctgaacttct ttacgaccgt
    14041 cggaggggat aatgaatcct ttgccgcttt tgcgatcaaa ggttttgaca attcctgtca
    14101 ttttacggga caaacaaatt ccttactgaa aatactgcgc tgcactatac ggggttaata
    14161 aaataaagcc agcgatattt aagaccgccg gacggctaaa ataaaatttg cttaatctca
    14221 attatcatgc gttaatagct gcgtcggttt gaaagacaga cagcatacaa agtagtttac
    14281 taaagcagtt ctcattatca ggcattatcc ccttcttttg agtctctctc ctgaacacta
    14341 agtagtttct gtattaaagc cctgtttgcc gaaaggccca aaatgaagga agtaaaatat
    14401 gtctaataaa atgactggtt tagtaaaatg gtttaacgca gataaaggtt ttggctttat
    14461 cactcctgat gatggcagca aagacgtttt cgtccatttc accgccatcc agagcaatga
    14521 attccgcacg ctgaacgaaa atcagaaagt tgaattttct attgagcagg ggcaacgtgg
    14581 ccccgcggca gcgaacgttg ttacgctcta aggttgccgt tattactcaa catctccatt
    14641 tccgctgtcc atgttgtcat ggttcacagt accgtacatc ggcattcgat gtgacggagc
    14701 gaaacccttt gggcgctaag tgtatttttt gtaaatcgac gatgatcacc tttgataacg
    14761 tcgcgctgca aatatgcacg gaccatgcgc cgctggattt cacaaaataa tatcaggctc
    14821 cctcgtggag ccttttttat atctgcctta tttttcttca acgctgtatg tatagtaagc
    14881 gataacctgt tgattattga atctttcggg gagatggctt ataacatttc ttacctgacc
    14941 agggtaccgg gaaccaacac cttactggcg tgttgctgtc tttcaagacc agaagaggtt
    15001 aacagtgaat attgaagagt taaaaaaaca agccgaaacg gaaatcgccg actttatcgc
    15061 gcaaaaaatc gccgagctga acaagaatac agggaaagaa gtctctgaaa ttcgcttcac
    15121 cgcacgagaa aaaatgaccg ggcttgaaag ttatgatgtc aaaatcaaaa taatgtgatt
    15181 ttgtgaacat cacccgtgcg aggtgatgtt ccgcttgttg ctaatttagt gaccaatcat
    15241 tggcgcttgt ggaattaagc gtcggtacaa ttcatccggt accgggcttt gccatactcc
    15301 cgcatacatt gcgtaaccaa tcaccgcaaa cataatcccc agaaccagta gcgtcattaa
    15361 ccagccagac aacgcaaagg cttttttatt tgccgcaggt ttttgcagtg aaaaggtcaa
    15421 tgttgaggct accggacatg actctacgca agtcatacag ccggtacatt ccactgttcg
    15481 tacctgaatt aatttatcga ccgggatccg tgatgggcaa ttttttgcgc atttgccaca
    15541 gtcgatacaa ctttcggcat tgcgacgaat cttaaacggc gacaatagcg aaaccacgcc
    15601 catcagcgcg ccatatgggc aaagataacg acaccaggca tggcgaataa acaggctggc
    15661 aatcagcaaa acggtcacgc tgattaatgt cgcggtcccc atatgacgaa agaaatcgag
    15721 cattttaacg tccatcacca cgctgtaggg cgacaacata aaatagtgaa tcgcctgagc
    15781 gggcatcaat aacgcgatat agagaaaaaa actcaacagc aaatacttca cgccgcgcag
    15841 aggaatatcc agccagcggg gaaggacaca ttgccgacca aacagtttgt taccgagatc
    15901 gccgattaat tcagaaagcg taccaaccgg gcataaccat gagcaaaagg cctttttgag
    15961 taatagactg atgacgataa aagcgaccaa taacagcatc gcggcggcgt ggacggacgg
    16021 taactgacct gttacaaggc tatatttcag attcatcagc ccggcaatcg gtagccagcc
    16081 ttcgatacct cccggtctgg cgacaaatgt cgtgctactt gccgtttcgt aatagcgcac
    16141 ccaataccag aacgtgatgg caatataaat attcattgcc aacagtaata attgcgtcgc
    16201 tttacgccag gtcgtggcat tacgccagtc attccacggt aatttgccgc ccgtcgtgcc
    16261 tggccgccgc tgccagcgga ttcttttatt ctctgccatg attttgccag tccgttaagt
    16321 tgtataccaa atgccactat tctagttgtt cttaactggc tgatattgat tcaaatcgcg
    16381 ttcaggtctt tcttatgcaa ccatgcttcc agagcggcaa cactgcgtgt aatttcttcg
    16441 tgtggaaggg gggcagataa tggctgctgc tccagttgtg cgcatagctg gctggcgata
    16501 cgcattccca gactcgagca actgcttttt agctgatgcg cggcacgctt tattttctcg
    16561 ctatcctgac tggcgcgggc aatgtcgatt tcatcgagaa gcggcagggc atgttgtgta
    16621 aataatacca gccattcgtg gatcttctcc gtccccatta actgagcatc ttcattgagt
    16681 tgcgatacat ccagcgattg atcgttattg acttgcagtt ggagatagtg cgccagtaac
    16741 tgaccgagca cttcacgcgg caccggttta gggataatcc cgcggaataa tgaactggta
    16801 cgctggcgca gcgtttcgtc aatgacatgg gcgctaaagc caatcaaaac cagcgacgga
    16861 tattgctgtg ccagttgtcg ggcaagcgta atgccgtcga catccggcag atcaaaatcc
    16921 accagtgcgg cagcaaacgg ttcgctattt tgcagtgtct ctaaagcctg cgcggcattg
    16981 ccaacagcaa caacctgcgc accactggtg ttcagcatct caacggtaat tcgctgggtt
    17041 agcgggttat cttcaattaa cagcaaacgt aaaccgtcaa gacgcaccgc ctgattgact
    17101 gtttttggca cgggtgccgt ggcaacacgt aacggcaagc gtaaacaaaa acagcttcca
    17161 acctccggcg tgctggtggc gctcagttcg ccgcccatcg cctgggccag acggctactg
    17221 atagtcagcc ccagcccggt gccgccgcgt ttgccgctta cctgcacaaa tggctggaag
    17281 atttctgcca gtttcgcggg atcaataccg cagccgctgt cttccacttc gaccagccat
    17341 tgctcgccat cagtgcgact acgcaggata atgtaccctt cgtcagtaaa acgcagggcg
    17401 ttgctcaaca ggttggttat aacctgacga atacgtcgtg gatcgcccat taacgcgcac
    17461 ggcatatcat cggcaattgc cgttgccagg cgaatcgggc gacctttcac ccgtccgctc
    17521 attaattgca gggtactttc cagcagcggg cgcggttcaa agggctcatc gctgaccgaa
    17581 acattcttgc cacctgcttc gatagcggaa taatcgagaa tatcgttgag gatggtcagc
    17641 aacgattcac cagagtcagt aattgcccgc aaatcatcac gctgggcgtt aagtgcgggg
    17701 ttatctgcca gcagttgagc agtgccgaga ataccgtaca gcggtgtgcg gatctcatgg
    17761 ctcatcgccg ccagaaacgc cgattttgcc tggctggctt tttctgcttc cgcccgtgcc
    17821 tgtcggtgtt ctatcaccag ttcctgcaat tcagctgtac gcgctttgac ctgcgccgcc
    17881 agctgttcgc ggtggcgatt cagtgcatga acattgctgc gaaacgcatc catcagccgc
    17941 ccgatggtat ccagctcccg tacgccagcg gtttccggga aaggggagtc aatatcaccg
    18001 tccagcagcc gttgcagcgc ctgcgtttgt tcggcaagtg gacgcgtgac tgagcgataa
    18061 accacgcgcc aaaggatcag aatcagtgcg caaagtgaaa ccatccccag caataacagg
    18121 ctgtattgcc cgcgtgcact ggctttttcc agatgcgcca gtccgtgctg attacgcagc
    18181 tcaatggtgt cgaccagctg actgacttcg ctactaaact gcgcgaactg ggcgatgtta
    18241 ttttgtgcga gagtttgtag gtgattgctg atttcactgt cctgctgata cagcgccagc
    18301 aaatcgctat attggctaac ggtagttaac gttgttgcga cctgcgcacg aacacccgga
    18361 tcttcaatgc gtatttgccg acgttgcaga attttcaccg cattattgag ctgcttttcc
    18421 agcgttggtg catttttctg gatctgctcc agccccagat tcatcaccat ttgctgcacc
    18481 cgcagagcgc taaggcgcag ttcattcatc tggttaacat actcaagatc gatatcaatc
    18541 agccgatcga gtgcactttc agcagcctga cgctgatctt gttcgatcaa atcgtaaatc
    18601 ccggcctggg tcgctccagc ggaagttgtc gcattattcg cctgaccttg cgccaggcgt
    18661 gcgatctcat cggcggcagc gactatctgc tgactgagtt gccgttgttg ctggcgtagt
    18721 tgcagacgcc gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc
    18781 tgttgttcaa tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta
    18841 atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg
    18901 gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc
    18961 gccatattca tggtaggaat caatgcctga gtgttgtctt tttccacctg gctgataaag
    19021 cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag
    19081 cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcacttta ggtgaaaaag
    19141 gttgagtcgc aaagcggaat gcatctagca taaagactta ttattgatga ggctatcatg
    19201 cgcgtactgc tatttttact tctttccctt ttcatgttgc cggcattttc ggctgataac
    19261 ctgttgcgct ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaaacc
    19321 aaacgcgcat ggaaactgtg cgcgctttat ccgagcctga aagattcata ctggttatcg
    19381 ttgaactatg gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag
    19441 gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg
    19501 ggcgcagagg ccattttgct cggtagtagc acgacgtcat ttcccgatct gcaaaagcag
    19561 gtagcaaatc tgccggtgat cgaactggta aatgctattg atgctcccca ggtgaaaagc
    19621 cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatatct ggtgcaatgg
    19681 gcgcacggta aaccactgaa cgtgctgttg atgcccggac ccgataacgc cgggggcagt
    19741 aaggagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat
    19801 attgcgctcg gtgataacga tattgaaatc cagcgtaacc tgttgcagga gatgctggaa
    19861 cgccatccag aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt
    19921 gaagggcgca acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg
    19981 tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag
    20041 ggggaactgg cggttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat
    20101 gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgtgaaca tattcgccgc
    20161 tcgctgtcac cagggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag
    20221 aaataacctt caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga
    20281 cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata
    20341 cgtagcagac gttcacggct gagaatttcg cccggattcg tcacaaatgc caccaacatt
    20401 tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccaacgt atggcgcgac
    20461 acattcaggc aataaccggc aaagcgatag cagttgtcct gagtgtgcgg ttgagcttgt
    20521 cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc
    20581 agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga
    20641 tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt
    20701 aacatcaggc cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga
    20761 ttctgcataa tttcccgcag cccggcaccg ctcgctgtaa cggaaacggt atacccctcc
    20821 tgagtgaagt aagattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg
    20881 tgatgtggca tcagagggtt ttactcattc tgttcatatc tgttcatatt ctgccgtaag
    20941 ccgttcatcc tgaccagtgc cgctgttcat atttgctcat taagatcgct tcactaaacc
    21001 ataattttac aggggctatt atgcggaaac tctggaacgc gctacgccga cccagtgctc
    21061 gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg ctgattgtat
    21121 tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta
    21181 tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc
    21241 gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac
    21301 tggaagcgag caatgatatc taccagacct ttattgctca ctccattgat acacctgaaa
    21361 aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca
    21421 actcggcaac ctgccgctcc tgccataact acgatgcgat ggatcatgcg aagcagcatc
    21481 ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc
    21541 ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg
    21601 agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa
    21661 tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga
    21721 aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg
    21781 ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc
    21841 gtggtgatgt gcagcagcag gtaaaaacgc tggagaaaac caccgttgcc gacaccaata
    21901 ccgagtggag caagttgcag gccaccgcat ggatgaagaa aggcgacatg gtgaacgata
    21961 tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg
    22021 gcgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga
    22081 ttggctttac cagtctcgat aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga
    22141 atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa
    22201 taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt
    22261 cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcgactgcgg cgcaagcggc
    22321 gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg
    22381 cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc
    22441 gtcgaaaatg attgccggat tgccggatca cgtacacaac gcggcgcgta ttcgttatcc
    22501 gatggtacgc gtggactggc tgcgtaagcg ccatctcagc gatacctccc agcgcggtga
    22561 taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga
    22621 acgcgtgcag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac
    22681 ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag
    22741 cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtggt
    22801 tggctcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa
    22861 aaccattgtg ctgtggggct ctgatttgct gaaaaaccag caagcgaact ggtggtgccc
    22921 ggatcacgat gtttatgaat attacgcgca gctaaaagcg aaagtcgccg ccggtgaaat
    22981 tgaggtcatc tgcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca
    23041 tgtgaagcac attgcggtta acccgcaaac tgatgtgccg ctgcaactgg cgctggcgta
    23101 tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt
    23161 tgagcagttc ctgccgtacc tgctgggtga gaaagacggt cagccgaaag atgccgcatg
    23221 ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc ggcagatggc
    23281 ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca
    23341 gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg
    23401 tggctttggt tttggctggc actataacgg cgcaggcacg ccggggcgta aaggcgttat
    23461 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgattacaa
    23521 aggctacagc agcaccattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
    23581 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
    23641 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
    23701 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
    23761 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
    23821 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga
    23881 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgc gaagaagcct ttaccgaagg
    23941 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
    24001 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
    24061 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
    24121 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
    24181 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
    24241 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
    24301 gcagttatgt gagtcggaaa ctctgcgtca gcaatatacg gtagcgggta aagagccagt
    24361 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
    24421 ctttaacgct cgcggtcagg tgttggcagg ggcagtagtt tctgaccgct atgcacccgg
    24481 cgtggcacga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    24541 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcggtactt cgcagctggc
    24601 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gagcagtgga
    24661 gcaggtgacg gcgtttaacg gccctgtgga gatggtggcg cagtgcgaat atgttcccgc
    24721 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    24781 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    24841 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctggctgc ggcggtgaat
    24901 gagctggaaa actgtattgc cacgctgaca gtacgtgacg atgcccgtct ggaactggcc
    24961 gctgactttt gcggcctgtt tctgatgacc gacaaacaag cggcgctgcc gtatgcatcg
    25021 gcctacaaac aggacgagca agagattaaa cgcttgttag ttgaggcagg gatggaaacc
    25081 agcggcaatt tcaacgaacc ggcagatcat ctggcgatct atctcgaatt gctcagccat
    25141 ctgcattttt cgctgggaga ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa
    25201 aaaacactga cggcgctgtg gcaatggtta ccagagtttg ttgtgcgttg tcgtcagtat
    25261 gacagctttg gtttttacgc ggcactaagc cagttattgc tggtgttagt ggagagcgac
    25321 caccaaaaca gataacgtcg tttgtgcgcc tgaaaagacg cgtttagcgt cgcatcaggc
    25381 attatggggc agttgccgga tgcgtcatca catccggcaa acacacatca cggatgagct
    25441 acaaaccggg aaagtcgctg gcgcagcagg cggttttcct gcttcaggtg cgcaatatca
    25501 tccattaacg tcagcgccac cgcgatccca ggccagtcca gagccagttc atgacgcagg
    25561 cgtactgcgc gttgcaccac gatggcggca tggtcgtcaa atacccaggt tgtttcctgg
    25621 atctcacgcg gttcaaccac ccccaaaccg acaatttcat tcaactcctc ttcagagatg
    25681 ccggtatgca ggcaaaattc ggtaatagta aaagtcaccg taacattagc cattatgctt
    25741 tcccccaatc tttacgtgga tcaaaagacg actgggcgtc tgccagttgc tgccacagcg
    25801 cggcagtgtt ttcatccggt ttcggcggca tcacgatttt cagtaccgca tacagatcgc
    25861 cggtctgttt tttgctcacc agacctttgc ctttaacgcg caatcgttgc ccggcctggc
    25921 tgcctggcgg gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg actttagcac
    25981 ccagcgccgc ttcccacggg ctaaccggca ccacaatttc cagatcctgg ccgacaatat
    26041 cgaacagcgg atgtggcgca atatgaatca ccagccacaa atcgccattt ggaccgccgt
    26101 tttcgcccgg cgtcccctgg cctttcaggc ggatgcgttg accattgccg acgcccgccg
    26161 ggatcttcac attcagcgtt ttcgggattt cctgttcgat catgccaaag gcgttataaa
    26221 ccggcaggtt atagctgatg gtacgcttat gctcagtaag cgtctcttcg aggaataccg
    26281 ccacttcgat ttcaatatcg tggccgcgtg tggcggggcg ttgacggctc tggcgggcat
    26341 gctgaccgaa aattgacgag aagatatcgt caaaatcttc ggcgttaaaa ctctgaccgt
    26401 ctccatgctg gaactgacgg ctaaattgtg gatcgttgcg atgttgccac atctgatcat
    26461 actcagcgcg acgttgttca tcactcaaca cttcccaggc ttctgcaacc tctttgaagc
    26521 gggcttcggc atccggttct ttgctgacat caggatggta tttgcgggcc agtcgacgat
    26581 aggcggtctt gattgtcttg agatcgtccg tcggtttcac gcccatgatg gcgtaataat
    26641 cctttaattc catagcgtta tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag
    26701 gtaactccta taagtgtagg gtaatcctca aaatttcata tgccaacaca gaatatgtta
    26761 ttgaaatcat cgcggagagg aggtcgcctc aaggtggggt gtcgaacata ttttaaacag
    26821 gtgaaaaagg gtgagcgatt tttgatagtt gaaacaggca ctttaagttt aactggggcg
    26881 tcattattta ttaaatttta taggcgctat atatgggtaa tatacatgga attagttgca
    26941 ctgcaaataa ttatttgaag caagcctgga acgatataaa aaatgagtac aaaaaaaatc
    27001 aaacatattc aatcacgctt tttgaaaaca cactggtgtg ttttatgcgg ttgtataaag
    27061 aaatcagacg tcaacaagct gaagactaca ttccatgcct ggaatgcgat tcactagaaa
    27121 aagaatttga ggaaatacag aatgataatg atttatcatt atttatgaaa acattgcgta
    27181 ctaatgataa acaaatttat tcaggggttt caggaggtat tacatatact atacaatatg
    27241 ttgaagatgt tgatattgtt agagtgtcct taccgggcag agggtcagag tctatcacag
    27301 attttaaagg ttattattgg tatggcttta tggaatatat tgaaaacatt actgcgtgtg
    27361 atgatgtttt ttctgagtat tgtttagatg atgaaaatat aagtgtccag ccagagcgga
    27421 taaatacgcc gggaatatct gatttggatt ctggcattga tttgtctggt atatctttta
    27481 ttcagagtga aattaataaa acttatggat taaaatacgc tccagtagat ggcgatggct
    27541 attgtctgtt aagggccata ctggttttaa aagaacatga gtattcatgg gcgctgggca
    27601 gccataagac gcaaaagcaa gtatacgaag agtttattaa aattgttgat aaacaaacga
    27661 tcgaggctct tgttgatacg gcattctata atctcaggga agatgtaaag acgttatttg
    27721 gcgttgatct acaatctgac aacaaaattc agggggaggg tagtcttatg ccatggaact
    27781 ttctgttttt taaaaaggaa tttattgata gttgcttgaa tgacaaaaaa tgcatactgc
    27841 atttacccga gtttatattt aatgataaca aggcacgcct tgttttagat accgacccag
    27901 agaagaggat taatgaggtg aaaaatttcc tcacggctct ttcagatagc atttgctcat
    27961 tatttattgt taatagtaat gtggcatcaa tctccttagg gaatgaatcc ttttcaacag
    28021 atgaagatct tgagtatggt tatttaataa acactggcaa tcattatgac gtttacctcc
    28081 ctcctgaact ttttgctcag gcttacgagt taaacaataa ggaaaggaat gcgcagattg
    28141 attttttaac tcgttatgca atttaatgac aaaggcatgt gctaaaaacc attgttatta
    28201 atctcacact tttttattgg taaatattgc ctctgtattg gtaacgccgc tgatattctg
    28261 tctagccctc tggtgtaatc atcagcggcg ttcgtgaggc agggagctaa tcaggcatag
    28321 tttgcgtcaa accttgcctg tttttaaaga tgtatataga aaaacaggca ttcaacaagc
    28381 cattttgcga acctgttccc ggaaaaatgt catatttctg tcacactctt tagtgattga
    28441 taacaaaaga ggtgccagga atgaacaaaa cgctaatcgc cgcaactgtg gcagggatag
    28501 ttttactcgc ttcaaatgct caggcacaaa ccgtaccgga aggctatcag ctacagcaag
    28561 tgctcatgat gagccgccat aacttacgtg cgccgctggc caataatggc agcgtgctgg
    28621 agcagtcaac gccgaataaa tggccagaat gggacgtccc cggtgggcaa ctcaccacca
    28681 aaggtggcgt gctcgaagtg tatatgggcc attacatgcg tgaatggctg gcagaacagg
    28741 ggatggtgaa atcgggggaa tgcccgccgc cggacaccgt ttatgcctat gccaatagtc
    28801 tgcaacgtac cgttgcgacc gcacagttct ttattaccgg cgcattcccc ggctgtgata
    28861 ttcctgttca tcatcaggaa aaaatgggca ctatggaccc aacctttaac ccagtgatca
    28921 ccgatgattc cgccgcattc agtgaacagg cggtggcggc aatggagaaa gagctcagca
    28981 aactccagct taccgacagc taccaactac tggaaaaaat cgttaactat aaagattccc
    29041 ctgcctgtaa agagaaacaa cagtgttcgc tggtggatgg caaaaatacc tttagcgcca
    29101 agtatcaaca agaaccaggt gtttccgggc cgctgaaagt cggcaactcg ctggtagatg
    29161 cgtttacttt gcaatattac gaaggttttc cgatggatca ggtggcctgg ggagaaatca
    29221 aatctgacca gcagtggaag gtgttgtcga agcttaaaaa cggctaccag gacagcctgt
    29281 ttacctcacc ggaagtggcg cgcaatgttg cgaaaccgct ggtcagttat atcgacaaag
    29341 ctctggtcac cgatcgcacc agcgcaccga aaattacagt gttggttggg cacgactcca
    29401 acattgcctc tctgttaacg gcgctggatt tcaaaccgta tcagttgcat gaccagaacg
    29461 aacgcacgcc gattggcggc aaaatcgttt tccagcgttg gcatgacagc aaagccaatc
    29521 gcgatttgat gaaaattgaa tatgtgtatc agagtgcgga acagttacgt aatgccgatg
    29581 cgttaaccct gcaggcacct gctcagcgtg tgacgctgga attaagcggt tgcccgatag
    29641 atgccaacgg tttctgccca atggataagt ttgatagcgt gttgaatgag gcggtgaaat
    29701 aacagaaaac tcccccgcga gaagtggggg agtcgctggt taaacgtttt tacgttcgat
    29761 ggtctgttcg ccccaaaaaa gcgaatcttt atcggtctta gcaaaggctt tgactaacac
    29821 ttcatcgcta ccttcttccc aaatcttttc cgccattttt tcgtcgtacc cggcgacttc
    29881 gaaaatggcc tcggctattt ccggcgacgt attgcgcaga gatgcccatt caccgacgtg
    29941 atgagctttc gcttcttgag ttggcatgcg tatcctcctg ttgaagatta gccgttaagt
    30001 ttaactgcca gacctgcgac atattcccct tgataacgag caatagacag ttcttcctgg
    30061 cttggctggc gtgagccgtc accgcctgcg atggtggttg cgccgtacgg cgtaccgccg
    30121 cgaacctgtg aaacgtcaaa taattcctgc gctgcgtagc caatggggac aatcaccatg
    30181 ccgtgatgcg caagggtcgt ccaggttgaa gtaatagttt gttcctgacc gccgccagta
    30241 ccggtggaac taaagacgct cgccagtttt ccgtatagtg cgccggaagc ccacaggccg
    30301 cccgtctggt cgaggaaggt acgcatttga ccggacatgt tgccaaagcg ggtaggtgta
    30361 ccaaaaataa tggcgtcgta atcggccagt tcttgcgggg ttgcaaccgg tgcagtttgc
    30421 gttttaccgc ctgctttttc aaataattgc ggcggcatgg tttccgatac gcgcttaacg
    30481 acaacttctg cgccatcgac tttgcttgca ccctcagcga ctgcgcgtgc catcgtttca
    30541 atatgtccgt acatggaata ataaagcacc agaactttag ccatttctaa ccactcctcg
    30601 tgttatctct attccgtagc gattcgctac cacttattta aagataagac gtccttttca
    30661 gagtgcaaat ttcacaacca cttatttgat ttataacaac tttcacaagc acgtaatttt
    30721 gtcgcaaaat gacacatttt tatctcatcg cgttttttta atcataagag cggcttatgg
    30781 ataattattg gagatgatat ctattctcgc taataagctg ttgcaggata ttaccaaacg
    30841 cgggtctgcc cgcgtcagtt cactaagctt agtcccacgt agcgaaaata tggcagccgc
    30901 catacgccgc gttaattcta tgcaatatga tgtctatacc cagacggagg tcagtaatgg
    30961 caaaccatcg aggcggttcc ggcaattttg cagaagaccg cgaaagagca tcagaagcag
    31021 gtaaaaaagg tggacagcac agcgggggta atttcaagaa tgacccgcag cgcgcatctg
    31081 aagcaggtaa aaaaggtggc aagagcagtc acggcaaaag cgacaactag ccgggctaat
    31141 caatgacgaa tgcatttttt tcttaggctc gtcaaaaagt catcaccgcc ggtaatcccg
    31201 gcggttttct gtttatggct cctgatgaac aacttctggc ggtggaacgt caaccaacct
    31261 tctgcttaac aacgcattga gtaaaatcgc gccaaaggtt gctgtaccga tccctcccaa
    31321 cgtaaaaccg cccagcgtga gagcaaaatc acccgcgccc agcactaagg ttactgcgac
    31381 cataatcaaa ttaccgttct ggcttaaatc gacacggttt tgtacccaga tccttgcgcc
    31441 tgcgacggca atcagcccga acacaacaat tgaagcacca ccaatcaccg gtgcaggaat
    31501 ggtatggatc agcgcgccaa atttcggcga aaagcccaac agcatggcga tgacggcagc
    31561 agcaacaaac accagcgtcg agtagacttt ggtcacggcc atcacaccga tattttcagc
    31621 ataggtggtc acgccgctac cgccgacaga gccggaaagc atcgttgcca gaccatcgcc
    31681 tacgaatgcc cgccccatat acgggtccat attgcgtccg gtcatcccgg cgactgcctt
    31741 gagatgacct aagttttccg ccaccagaat caccgccacg ggcgcaatca gcatcattgc
    31801 ctgaccatta aaagcaggag tggaaaaatg tggcagaccg aaccaggcag catggctgac
    31861 gagagtaaaa tcgacggctt ttcccagccc taaaacgttg gtcatcacgc catacagcag
    31921 acaggcgaca attaaaccta cgagaatcaa taaccgctgg atcatgccac gggtaaacac
    31981 cgccaccagc ccaatacaca gcaccgtcat taccgccatc cagctatcaa aggccgaagc
    32041 cgatacactt ttcactgcga taggcgctaa gttcaggcca atcgccatca ccaccgcacc
    32101 cgtcaccacc ggcggcatta gtcgttcaat ccagcgtgta ccaattttca tcaccaccag
    32161 gccaatgacg gtataaacca gcccacaggc gataatcccg cccagcgcaa tgctgatatt
    32221 cgggttaatg ccctgaccgt taaagcccgt cgcggcaatc accacgccga caaaagccgc
    32281 gctggaaccg agataactgg ggacgcgccc gccggtaata aagaaaaaca gtaacgtgcc
    32341 gatccccgac attaaaatgg aaagattggg atccagcccc atcagaatcg gcattaacac
    32401 cgtcgcgcca aacatcgcca ccgcgtgttg aacgcccatt actgccgtct gagcaaacgg
    32461 caatcgttca tccggcgcga ccacgccgct ctctatagag gtcgatttta actgccagtg
    32521 aggaaaaccg aacattgcca tcagctgtct ccttaaggag gttaacaagc agggcgcatc
    32581 agcgcgtgat aactgcgatc gaaccacacc agcccgtagg gtgtggcgtg acgatgaatc
    32641 gcttcgatgg cgcaaaacaa aatgtcgtgg gtgccgacgc tcaccacttg gctgatacgg
    32701 cagtcaaacg aaaccagtgc ctcttccagt tgcgggcatc cggtcacacc cgtctgccag
    32761 cgggcggcgg caaagcggtg ttccatgggc gttttgccgc caaaaaggtt tgaaagcggc
    32821 tcctgcccgg cgctaagtgt atttacacat agcgttcgat tttcattgaa taccggccag
    32881 acggacgccc cacgattcag gcacaccagt aatgtgggcg gcgtatcggt cacgctgcag
    32941 accgcgctgg cggtgaaccc ggcgcgcccg gctggaccgt ccgtggtgat gatattgacc
    33001 gccgcgccca tacaggacat cgcatcgcga aaggtttgtt gatcaactat gttcatagtt
    33061 tgctccttac aacagcccgc aggcttcttc aaaggacaga cgtggcaggc gcgcataaag
    33121 tttgctgcta tcgccatagc cgatattaat cagcagattg ctcttcagcg tgctgcctgc
    33181 aaaaaaagcg tcgtcaacgt attgacggtc aaaacccgac atcgggccgg tatccagtcc
    33241 cagcgcacgg caagcgacga tcagataggc cgcctgcatg gagctgttgc gaaacgctgt
    33301 ttcttcggca agttgtgggc tggaggtaaa ccaactgcgg gcatcaccgt ggggaaacag
    33361 tagtggtaac cgttcataaa attcactgtc ccaggcaacg atagcggtga cgggcgcggt
    33421 cagggttttt tgtagattgc cgctggaaag cgccgggcgc agacgttctt ttccttctac
    33481 agtgcggata aacacgatcc gtgccggaga acagttagct gatgtcggcc cccatttcat
    33541 cagggcataa agctcccgta acgtctcatc gctgacgggt gtctcccgcc agccgttgtg
    33601 agtgcgggca tcggtgaaca gggtgctaag cgcacctggg ctaacggctt cgttcatagc
    33661 aattccttac agggcggctt cgcggtgatg taacaggctg gcaagcccgt tgagtaaaag
    33721 agcattaaac gtttcggggt cggtcacgtt gcaggcgtgt ccgccatagc gcatcaccat
    33781 tttctggcta tcgggcaggg cggcatgaag ctcactggaa catgctgatg gcaccagcag
    33841 atcatcactg gcgcagatga tttgcaccgg gcagcggatg cgatccgcat ggtgactaaa
    33901 gtcagcgcgt ttgagggcgt taagtcgacg cagtaaatta tttttgccct gaaaatgcgc
    33961 cagtgccagc gcgtcttctg cctcaaggcg aggtgcgcgg gccgccatcc agtcggcggg
    34021 atagaggaac aacggctgcg cttccaccca tgcctgcgcg ccgccgctat acagtaatcg
    34081 ttcgcgaacc tgaaaacagc ggcgcgtatg ggcgtttatt cgtagccagc cgttaacgct
    34141 gatcagcaca gttaccgacg cgggataatc cagcgccagc tgcattccca ccagcgcacc
    34201 gagcgcatgg ccgaccaccg cgtaatgctc aatccctgcg gctaccagcg cctgatgcag
    34261 ttccgctgcc atctgggcga tactgtaatc ttctgccagc gtgtcgggat tattgccggt
    34321 gccgcgctgg tcgtaacaga ctacctgata ctcctgctcc agcaccgcca gttgcggtaa
    34381 ccagtaactg ccgctaccgc caagacccga aatcaacacc actacgggcg catcagcata
    34441 agggggaggt gagagtgaaa gtttcatcgc ggcctcactt ggcgatatgc gcaattgtgg
    34501 cgatttccac cagcgcgtca ggttttacca gtccgcactg aatgcagaat cgcgccggtt
    34561 tatcacccgg aaaaaactcg gcgtagattt cgttaatcgc ggcgtaattt ttccagtcgg
    34621 taataaagat gctgttgaag gtcacatccg ccatcgtgcc acccgccgtc tcgatcacct
    34681 tgcggataat ttccagaacg tggcgggttt gcgcctttgg gtcatcggca aacagcacgt
    34741 tattatgttg atcaaaagcc agcgtaccgg agacatacac cacgccatca gccagcgtgc
    34801 cgggaacgaa gggggccagc ggtgcgctgc tgccagcggg aataattacg gattttggca
    34861 tcgttaaact ccttaagcga tacgagcaaa ggatgtggga gaaagcgcgt cgcagaacgt
    34921 ttcgacgtcg ctgacccagc caaaaaaggt ttcgatattg aacaacgcgg ctttctgtgc
    34981 aaattccggc cccgcctggt gtgttgcgtc ttccagcacc acgccgaaat actccagaaa
    35041 aaagccatcg cgtagcgtcg attcgacgca gacgttggta gcgataccgg tgaaaaccag
    35101 atggcgtatt ccacggctgc gcaaaatgct gtccagcggc gtattgaaga aaccgctgta
    35161 gcgcggcttc ggcagcacaa tatcgccagg ctgcggcacc agttcatcca ccagttgata
    35221 atcccaggag cctttcgcca gcaatttccc ctgcagctgc ggctgcttac gcatggtttt
    35281 cagggcgttc gatttatgaa aattcggtga gccggggccg ccagcttcga catactgttc
    35341 atcccagcca ttttgaaacc agatgatcag catccctgcc gctcgcgctg cggtcacggc
    35401 ggtttgaatg ttggcaatga ccgggcgagt ggttgagaca tcaaacccgg cgagatctaa
    35461 gtagcctcct ggcgtggcat aagcgttttg catatccacc acgatcagcg cactttgctg
    35521 cggatcgaag gtaatggctt ccggtcgagc gg
//
LOCUS       DAEVZA010000036        34990 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEVZA010000036 DAEVZA010000000
VERSION     DAEVZA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.036"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..606)
                     /gene="flgH"
                     /locus_tag="LAF92_001855"
     CDS             complement(<1..606)
                     /gene="flgH"
                     /locus_tag="LAF92_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBI9790874.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVAD"
     gene            complement(659..1441)
                     /gene="flgG"
                     /locus_tag="LAF92_001856"
     CDS             complement(659..1441)
                     /gene="flgG"
                     /locus_tag="LAF92_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBI9790875.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(1613..2368)
                     /gene="flgF"
                     /locus_tag="LAF92_001857"
     CDS             complement(1613..2368)
                     /gene="flgF"
                     /locus_tag="LAF92_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HBI9790876.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(2388..3596)
                     /gene="flgE"
                     /locus_tag="LAF92_001858"
     CDS             complement(2388..3596)
                     /gene="flgE"
                     /locus_tag="LAF92_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBI9790877.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGADPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(3621..4316)
                     /gene="flgD"
                     /locus_tag="LAF92_001859"
     CDS             complement(3621..4316)
                     /gene="flgD"
                     /locus_tag="LAF92_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBI9790878.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(4328..4732)
                     /gene="flgC"
                     /locus_tag="LAF92_001860"
     CDS             complement(4328..4732)
                     /gene="flgC"
                     /locus_tag="LAF92_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBI9790879.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQSPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(4736..5152)
                     /gene="flgB"
                     /locus_tag="LAF92_001861"
     CDS             complement(4736..5152)
                     /gene="flgB"
                     /locus_tag="LAF92_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBI9790880.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            5308..5967
                     /gene="flgA"
                     /locus_tag="LAF92_001862"
     CDS             5308..5967
                     /gene="flgA"
                     /locus_tag="LAF92_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBI9790881.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPLRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            6043..6336
                     /gene="flgM"
                     /locus_tag="LAF92_001863"
     CDS             6043..6336
                     /gene="flgM"
                     /locus_tag="LAF92_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBI9790882.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDVPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            6341..6757
                     /gene="flgN"
                     /locus_tag="LAF92_001864"
     CDS             6341..6757
                     /gene="flgN"
                     /locus_tag="LAF92_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBI9790883.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGCKKISI"
     gene            complement(6797..8332)
                     /gene="murJ"
                     /locus_tag="LAF92_001865"
     CDS             complement(6797..8332)
                     /gene="murJ"
                     /locus_tag="LAF92_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBI9790884.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(8442..9365)
                     /locus_tag="LAF92_001866"
     CDS             complement(8442..9365)
                     /locus_tag="LAF92_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI9790885.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(9367..10014)
                     /locus_tag="LAF92_001867"
     CDS             complement(9367..10014)
                     /locus_tag="LAF92_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBI9790886.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTSGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(10025..10609)
                     /gene="rimJ"
                     /locus_tag="LAF92_001868"
     CDS             complement(10025..10609)
                     /gene="rimJ"
                     /locus_tag="LAF92_001868"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBI9790887.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            10845..12053
                     /gene="mdtH"
                     /locus_tag="LAF92_001869"
     CDS             10845..12053
                     /gene="mdtH"
                     /locus_tag="LAF92_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBI9790888.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            12117..12764
                     /gene="grxB"
                     /locus_tag="LAF92_001870"
     CDS             12117..12764
                     /gene="grxB"
                     /locus_tag="LAF92_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBI9790889.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            12898..13458
                     /locus_tag="LAF92_001871"
     CDS             12898..13458
                     /locus_tag="LAF92_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI9790890.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            13564..14610
                     /gene="pyrC"
                     /locus_tag="LAF92_001872"
     CDS             13564..14610
                     /gene="pyrC"
                     /locus_tag="LAF92_001872"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBI9790891.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            14684..14929
                     /gene="dinI"
                     /locus_tag="LAF92_001873"
     CDS             14684..14929
                     /gene="dinI"
                     /locus_tag="LAF92_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBI9790892.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            15219..15473
                     /gene="bssS"
                     /locus_tag="LAF92_001874"
     CDS             15219..15473
                     /gene="bssS"
                     /locus_tag="LAF92_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBI9790893.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            15588..16706
                     /gene="solA"
                     /locus_tag="LAF92_001875"
     CDS             15588..16706
                     /gene="solA"
                     /locus_tag="LAF92_001875"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBI9790894.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            16754..16867
                     /locus_tag="LAF92_001876"
     CDS             16754..16867
                     /locus_tag="LAF92_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBI9790895.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            17128..17694
                     /locus_tag="LAF92_001877"
     CDS             17128..17694
                     /locus_tag="LAF92_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBI9790896.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            17698..18273
                     /locus_tag="LAF92_001878"
     CDS             17698..18273
                     /locus_tag="LAF92_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBI9790897.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(18315..19367)
                     /locus_tag="LAF92_001879"
     CDS             complement(18315..19367)
                     /locus_tag="LAF92_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBI9790898.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            19592..20512
                     /gene="lpxL"
                     /locus_tag="LAF92_001880"
     CDS             19592..20512
                     /gene="lpxL"
                     /locus_tag="LAF92_001880"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBI9790899.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            20684..21910
                     /gene="mdtG"
                     /locus_tag="LAF92_001881"
     CDS             20684..21910
                     /gene="mdtG"
                     /locus_tag="LAF92_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HBI9790900.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            21993..22367
                     /locus_tag="LAF92_001882"
     CDS             21993..22367
                     /locus_tag="LAF92_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBI9790901.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(22368..22595)
                     /locus_tag="LAF92_001883"
     CDS             complement(22368..22595)
                     /locus_tag="LAF92_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBI9790902.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(22768..25311)
                     /gene="mdoH"
                     /locus_tag="LAF92_001884"
     CDS             complement(22768..25311)
                     /gene="mdoH"
                     /locus_tag="LAF92_001884"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBI9790903.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(25304..26839)
                     /gene="mdoG"
                     /locus_tag="LAF92_001885"
     CDS             complement(25304..26839)
                     /gene="mdoG"
                     /locus_tag="LAF92_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBI9790904.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            27233..28390
                     /gene="mdoC"
                     /locus_tag="LAF92_001886"
     CDS             27233..28390
                     /gene="mdoC"
                     /locus_tag="LAF92_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBI9790905.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(28398..29879)
                     /gene="clsC"
                     /locus_tag="LAF92_001887"
     CDS             complement(28398..29879)
                     /gene="clsC"
                     /locus_tag="LAF92_001887"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBI9790906.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(29821..30354)
                     /gene="ymdB"
                     /locus_tag="LAF92_001888"
     CDS             complement(29821..30354)
                     /gene="ymdB"
                     /locus_tag="LAF92_001888"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBI9790907.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(30449..30760)
                     /locus_tag="LAF92_001889"
     CDS             complement(30449..30760)
                     /locus_tag="LAF92_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI9790908.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(30881..31213)
                     /gene="csgC"
                     /locus_tag="LAF92_001890"
     CDS             complement(30881..31213)
                     /gene="csgC"
                     /locus_tag="LAF92_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBI9790909.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(31272..31727)
                     /gene="csgA"
                     /locus_tag="LAF92_001891"
     CDS             complement(31272..31727)
                     /gene="csgA"
                     /locus_tag="LAF92_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBI9790910.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(31768..32223)
                     /gene="csgB"
                     /locus_tag="LAF92_001892"
     CDS             complement(31768..32223)
                     /gene="csgB"
                     /locus_tag="LAF92_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBI9790911.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            32977..33627
                     /gene="csgD"
                     /locus_tag="LAF92_001893"
     CDS             32977..33627
                     /gene="csgD"
                     /locus_tag="LAF92_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBI9790912.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            33632..34021
                     /gene="csgE"
                     /locus_tag="LAF92_001894"
     CDS             33632..34021
                     /gene="csgE"
                     /locus_tag="LAF92_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBI9790913.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            34046..34462
                     /gene="csgF"
                     /locus_tag="LAF92_001895"
     CDS             34046..34462
                     /gene="csgF"
                     /locus_tag="LAF92_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBI9790914.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            34489..>34990
                     /locus_tag="LAF92_001896"
     CDS             34489..>34990
                     /locus_tag="LAF92_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBI9790915.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQY"
BASE COUNT         8720 a         8975 c         8482 g         8813 t
ORIGIN      
        1 atccgccacc tgagtagacg gtacggtatt gctgccgctg atagtgcgtg gattaaccac
       61 gccagagaag cgaataaatt cggtaccctg attaatggca atctgttttt cacccaccac
      121 atgcaggttg ccgttgacca gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt
      181 attgctggca ttggccccgc cctttccgtt gaacgtgtta ccaccggagg cttcgacatc
      241 ggcacgagcg ttaccaaaca gcccctgcaa atagcgcggc acagtatcaa agccaaaatt
      301 agttttaccg tcacggctgg cattcgcaga ggagcttttg ctggcgctga cgttctcctg
      361 caacacgatg gtcagcgtat cgccaatatt gcgtggtcga cgatcttcaa acagcggttg
      421 atagccatag ttaatcggct gagcagactg gaaaatagaa ccgttggcga cgggcgtcgg
      481 accgggaacc ggttgtgcac tggtcgcccc ctgcaccagc ggcgtggagg gtatccaggc
      541 gcagccggtt agtgaaagca ccaacaagct ggaaatggca taagtatgcg cagcgttttt
      601 ttgcatggct atcatcttca aaaatcagta aaccggtgaa cctgccaccg gttaagcctt
      661 agagttgcgt cagtttttgc agcatctgat cggtggtgga caccgcttta ctgttgattt
      721 cgtaagcgcg ttgcacctga atcatattga ccagttcttc cgccacgttg acgttagacg
      781 tttcaacata cccttgatac agcagtcccg cgccgttcag gcccggcgtg ctttcgttcg
      841 gtgcaccaga ggattgcgtt tcggtgtaga ggttttcgcc aatgctctcc agcccggtgt
      901 cattcataaa ggtggtgaga ttgagctgcc caacctgaac cggagctgcc tggccttgtt
      961 gggttacgct gaccacgcca tcacgaccga tggtgatact taacgcattc gccggaatgg
     1021 tgatcgctgg ctgcacctga aaaccaccag ccgtcaccag ctgcccgttc tgatccacct
     1081 ggaaagagcc gtcacgggta taggctgatg aaccatctgg caacatcacc tggaaaaagc
     1141 cctgcccttt aatcgcgaca tctttgctgt tgttggtctg cgacaggttt ccctggctgt
     1201 gtaagcgttc agtggcgacc gggcgtaccc ccgtgccgat ttgtaatccg gagggtaagg
     1261 tggtttgttc ggaagactgt gcccccggct ggcgaatggt ttgataaagc agatcttcaa
     1321 acaccgcgcg ctgacgctta aaaccgttag tactgacgtt tgccaggttg ttggcaatga
     1381 cgtccatatt ggtttgctgg gcgtcaaggc ccgttttggc gatccataat gaactgatca
     1441 taaaatgtcc tgtagtggat tagtgacaaa gtgcatatgt tttcttgccg gatgcggcgt
     1501 aaacgcctta tccggcctac aagagctcgc aaaatcaata aattgcgatt gcccccatag
     1561 acccgataag cttgcgcatc gggcaactta tacttgtcat gtatcctttc aattaactca
     1621 tcgacagcag ttggttggca cggcctgcgt tatcatcgac gctgctgatc accttcatct
     1681 gcatttcaaa acgccgcgcg ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt
     1741 tactgccttc cagaaccccc gacatcacac gcaaggttgg atctgcctgg agtaccggcc
     1801 cacgcgtggc ctggctttct gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt
     1861 cgctgcccgt ggctttcacc agcttcagac gccccactgg cgcaaccgta tttgccggat
     1921 cgcccggatt gagcgccgag attgtgccat cggcagcaat cgtgatttcc gccccttccg
     1981 gcacagcaat tggccccgcc tcgcctatca ccggatgccc ctgaattgtc agttgcccgg
     2041 tgggatcaac ctgaatgctg ccattacgcg tatacccttc gctgccgtca gcggtctgca
     2101 cggccagcca gccatcctgc tgcaacgcaa cgtccagcgg gcgcgaggtg taatccatct
     2161 tgccgggcgt catgtctgca cccggtgttg acgccgttac caacgtgcgc gtaggcagtg
     2221 aaagcccttc caccggcact gcgcgcaagg cgtttaactg cgcacgaaaa ccgggcgtag
     2281 aggcattggc cagattactg gcggttaccg cctgttgatt cagcgtctga ctggctgctc
     2341 ccatcgcggt ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta
     2401 accagcgtgt tgaggatctg gtcctgggtt ttgatggtct gggcgttaga ctgatagtta
     2461 cgctgggcaa cgatcatatt gaccagttct ttactgagat cgacgttgga cgcttccagc
     2521 gcaccgttgg tcagggtgcc aaagttgccc gtcccggctg tccccaacag cgccacgcca
     2581 gaagattgcg tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca
     2641 aagttcgcca gtacaatctg ccccagcagt tgggtttgtt cgttggaata gttgccgaca
     2701 accgtaccgt catcattgat ttgataactc acgagatcgc ccggtttgta gccgttctgg
     2761 gtggttgcca caatgttgtt agcgccggta ttttgctgca tggagttgag gaagctcagg
     2821 ctaaacgtgg cgggatctgc gccgttaatt gcgccggttg cgatattatt cgccatcgca
     2881 ccatccacta atgtgccatt agcattaaat tccagtgttg tcgctgtctt cgcaatgctg
     2941 tttggatcac tgctatcctg ggtgtagacc tgccagttat tatccccggt cttcacaaag
     3001 tacacgctca tgtcatgagc attaccctga ctgtcgaaaa cagtcaccga accttttttg
     3061 ttatagctat ccgcattgct ggcgctgaat ggcgtaacag taggaagcgg atcactggaa
     3121 ttcaggttga tctgcatcga cgccgtggtg gtagttttcg ctgccatcag ggtattcggg
     3181 atcgaaatat tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg
     3241 taacccgtca gctgtaaacc ttgcatattc accaggttac ggttctcatc cagcttaaat
     3301 tgtccgttac ggctgtagaa caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc
     3361 tggctgatag caacgtccag accacgcccg gtgttggtgg tcgtgccatc ggtaaagtcc
     3421 tgagtgatac cggcaacttt tacccccagt cccactttcg aaccggcaaa catatcggca
     3481 aaagaggccg tgcctgattt aaagccgtag gtggcggagt tggcgatatt gttgccaata
     3541 acatcgaggt tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa ggccatgact
     3601 gactcctgaa atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg
     3661 taagtgccga gatccagcgt attaccgttg ttgccgcgga tcacaccctg caccagagca
     3721 aactgcagcg gctgggcaac caattgtgta ccaccgttac tggcgctaat cgctacgttg
     3781 taagaaccgt tcggcgcagt gctgccgtca gtcaacgtac cgtcccaggt gaaactgtga
     3841 actccggcgg tcagttcacc aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg
     3901 atggtggccg taactttgtc tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg
     3961 gtcacagccc cttcttcact gccggttccc gcaagaacag tggtgccggg gatcatcacg
     4021 ccgtgaccga tcaggttact ggcctgtaac gactggctgt tatcaatctg tccggaaata
     4081 gatccgagcg tggtattgag tttttcaatc ccgctgaccg tgctgatttg tgccaattgc
     4141 gacgtcagct cgttgttttc cattggattg gtcgggtcct ggtttttcag ctgcgccacc
     4201 agcaaagtta gaaaactgct ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg
     4261 ctggtggtac tgacgccggt atttgtcgga tcggtggtgg ttaccgcaat ggacatagct
     4321 ttctccttta ttgaccgagc gtaagggttt tcagcatcat gcttttcacc gtgttgagca
     4381 cttcaacatt ggcctgatag ctgcgtgacg ccgacatggt gttaaccatc tccccgacga
     4441 catcaacgtt cggcattttt acgtagccct ttgcatccgc cagcggatta cccggttcat
     4501 aaaccagttt gtccggggac tgactttcta taacatcggc aacctttacg ccgcctgtcg
     4561 cagcacctgg tgcagcgtta acctggaata ccacctgttt tgcccgatat ggctgtccat
     4621 caggaccggt cacgctatca gcattcgcca gattactggc cgccacgttc aggcgctggg
     4681 actgggcagt taacgccgac ccggcgatat caaaaatatt cagcagtgcc atccgttaat
     4741 ttccgctctg taaaacgttc atcatgcctt tgatttgccc gctcaacgcg ctaaggctca
     4801 tctggtattg caggctgtta tcggcaaact gggtgcgttc gcgatccata tcgacggtat
     4861 taccgtcaag cgaaggctgg tccggaatac ggtattgcag ttctgcggta ggaggcgtca
     4921 gcgcctgcgc cggaatgtgt tgcgttgagg tcatcgtcag tgcaaccaca ctggttgcat
     4981 cccgtccacg ttgcatgact tttttaagtt cactggcaaa atcgatatcg cgcgcctgat
     5041 aaccaggggt atcggcattg gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca
     5101 gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc atatctcctc
     5161 cgcaggtatc aaaattctgc catcagctta aatgccttta cgcgcgcgtt atcggcggaa
     5221 taaacgcaaa atgggtcgct atttatgccg ttgatgacca ttgcggacag gtacaattca
     5281 cgttgtagaa atggctgggg ggcgaaaatg ctggcaataa aacgtagcgt ggcgatcatt
     5341 gcgatactgt tcagtccgtt aagtgcggcg agcaatctca catcgcaatt gcaaaccttt
     5401 tttagcgccc agctcgcggg ggtaagtgat gaggttcgtg tttctattcg tacagcgccc
     5461 aatctactac cgccgtgcga gcagccattg ctttcgatga gcaataattc ccgcctgtgg
     5521 ggcaatgtga atgtgttggc acgctgcggt aacgacaaac gatatttaca ggttaatgta
     5581 caggccacag gaaattatgt ggttgccgcg atgcccattg cgcggggagg aaagctggaa
     5641 gctggcaatg tcaaactgaa acgcggacgg ctggataccc tgccactgcg tacggtgctg
     5701 gatatcaatc aacttgttga tgccattagc ctgcgcgatc tatcacccga tcaacctatc
     5761 cagttaaccc agtttcgcca ggcatggcgg gtaaaagcgg gacaacgcgt caatgtgatc
     5821 gccagcggtg atgggtttag cgccaacgca gaaggtcagg cgctgaacaa tgcagccgtc
     5881 gcacagaatg cgcgggtgcg catggtatcg ggacaggtag tcagcggcgt tgttgatgca
     5941 gatgggaata ttcttataaa cctgtaagct gttaaagatt acccgtccct tgccgataaa
     6001 taagcaacac atgataaaag cgccctcaat gaggaataaa ccatgagtat tgatcgcact
     6061 tcgcctctga agcccgtaag caccgttcaa ccgcgcgaaa ccactgacgt gccggtaacg
     6121 aacacccggg cggcaaaaac aacagcatcc accagcacca gtgtgacgtt aagcgacgcg
     6181 caagctaaac tgatgcaacc cggcagcagt gatatcaatc ttgaacgtgt cgaagcgtta
     6241 aaactggcga ttcgtaacgg tgaactaaaa atggacaccg gcaaaattgc cgatgcgctg
     6301 atcaacgaag cgcagcaaga cttgcagagt aattaatcgt atgacgcgtc ttgcagagat
     6361 cctcgaccag atgtccgctg tgcttaacga tctcaaaacg gtaatggatc aagagcagca
     6421 acatctctct atggggcaga tcaacggcag ccagttgcaa tggattacag aacaaaaaag
     6481 ctcactgctg gcaacgttgg attacctcga acagttacgc aggaaagaac ccaatactgc
     6541 aaatagcgtt gatattagtc aacgctggca ggaaattgct gtgaaaacgc agcaactacg
     6601 ccaaatgaat caacataacg gctggttact ggaaggacag attgagcgca atcaacaggc
     6661 gctggaaatg ttgaaaccgc atcaggaacc gacgctatat ggggcgaacg gtcagacctc
     6721 aacaacccat cgcggctgta aaaagatttc gatctgaaga ttatctccgg cctgcactgc
     6781 aggccggaat gcattgttac accgtccggc gggcaaattc tttaactttg aagcccagta
     6841 ccgccagtgc agcgaagtac gcggcaatcc ccgccagcac gaccgccatt aaacgcagta
     6901 aacgccaggg catggtaccc aatgaccact ccggcatgat atgtaacata cctaaaagca
     6961 cgccagacat caccagtacc gccaccacca gacgcaacag aaacgccatc cagccgggtt
     7021 gcggagtaaa gattttctgc ttacgcaact gccagtaaag cagcgaagca ttcagacacg
     7081 ccgccagacc aatagaaagt gacagcccgg catgtttcaa cggaccaata aacgccaggt
     7141 tcatcaattg cgttaaaatc agcgtaacga tggcaatttt cactggcgtt ttaatgtcct
     7201 ggcgggaata aaagccagga gccaacactt tcactacaat caggccaatc aaacccaccg
     7261 agtaggcaat taacgcccgc tgggtcatca gcgcatcaaa cgcggtaaat ttaccgtact
     7321 ggaacagcga aacggttaac ggacctgaaa gaatgcccaa cgcaaccgca ctcggcagcg
     7381 ccaacaggaa acaaagacgc aacccccagt ccatcaaacg gttgtattca tcatgattgc
     7441 cactggcaaa acttttcgac agcgacggca gcaaaatggt gccaagcgcc acgcccagca
     7501 caccggacgg aaactccatt aagcggtcgg cgtaatacat ccaagacacc gaaccggaag
     7561 caagaaacga ggcaaaaatg gtgttgatga ttaaggagat ctggctcaca gagacgccaa
     7621 ggatcgccgg tcccatctgt ttcaccacgc gcattgctcc ggcatcgtgg aagttaatgc
     7681 gcggcaggac cagcatgccg atcttcttca ggtgcggtag ctgatacacc agctgcagga
     7741 tgccgccgac cgtaacagcc cacgccagcg ccagcaccgg tgggttaaag tacggtgcgg
     7801 caaacagcgc gaaaccaatc atgctgatgt taagcagtgt tggagcaaac gccggaatcg
     7861 agaagcggtt ccacgtattc agaatcgctc ccaccagcga cgccagggag atcagcaaga
     7921 tatagggaaa ggtaatcttt agtagctggc tggtcagggc aaatttgtca gctgtgtcag
     7981 cgaagcctgg cgcggtcacc atgatcaccc acggtgcggc gagcatgcca gcgaccgtca
     8041 caaccgccag cgcgagtgtc agcaggccag aaacataaga gacaaagacc cgcgtggcgt
     8101 cttcaccctg cttactttta tattccgcca gaatcggtac aaatgcctgg gaaaatgccc
     8161 cttcggcaaa gatacggcgt aacaagttag gaagtttaaa agcgacgaaa aaggcgtcgg
     8221 ttgccatccc tgcgccaaag attctggcga caattgcgtc tcgtgcgaag ccaagcacac
     8281 gcgaaaacat ggtcatcgag ctgacggcgg ccagcgattt taataaattc atcggtgttc
     8341 taatccagac ccacgggcaa aacgcctgca aggcaggcgc tgaaagaggc gaatagtcta
     8401 cctggattat ggtgaattgc taccgccaga tgttacaggg tttattcact catcgcatcg
     8461 cgccagatct tgtcaacgat acgttgcgcc agcacggcct gttcgccggc ggtttgcgga
     8521 actgtctgat tttgcacaca ttcaatgaag tgccgcgcac agccgacaaa tccacgttgt
     8581 tcaagagtgc tctgccaacc aggaatcggt ttatgcacta cgccctgccc gcgctcctca
     8641 cgccattcgc gcatatccgt aatgtcgatg agcgcaccgt cagtcacggc ctgcacggtt
     8701 tcacgctgac ttccggcacg gcgatgcata caggtggtga tctgcaaagg accggccgaa
     8761 aaatggtgct cggcaaacag catttcgcca gcgtcgttag tcagtagcgt accgccatcc
     8821 agagaggctt tgccgcccga caaccacagc gcggtatcca ccacatgcag ataatcatcc
     8881 agcaacgtga aataaagatc gtgtggcccg acactattgc tacgatgttt atccattctt
     8941 agcgaagctg cggtagcgag ttgcgttttt aactcaccgt agagtggtgc gaaacgacgg
     9001 ttaaaaccga ccatcaacgt cagtttttta cgcgccgcca gttccaccag ccgttcagca
     9061 tcgcgcagat tttctgccag cggtttatcg acacagacat gtacacccgc attgagtaac
     9121 gtactgacca cgtcaaagtg gctggcggtg ctggaatgca caaaaaccgc atcgcaactg
     9181 gcggcaaggc tggataacga atcggcataa ggaatgcgcc agctttcaca aattggcagg
     9241 gctttcgcgc gcgtaggcga ccaggctcct tgtaacatcc agtcagacgc tgccgccagc
     9301 accggtaacc acgctttttg cgcaatgccg cctaatccca caacgccgat acgtaatttt
     9361 ttcactttaa tctcccagat gggccagcaa cgaatcaaga cgctgtttca gttctgccac
     9421 ttcgatttcc agggcttcga cgcgggcctg taaatcacca tcaaccgcgt tcgacatatc
     9481 cgttaccgcc ggctgatcct ctacctcacc actgaaaaga tgcatgtagc ggctttcgcg
     9541 tttacccggt tcgcgggcca gacgcaccac aaaaggacca tcttcgcgat ttgccagttg
     9601 ttccagcgtc gactccactt ccgccatatc gctgaattca tacattcgcg cggcgcggct
     9661 gcgcagttca cctgacgtct gggcaccacg caataacaac gtggtgatta acgccacttc
     9721 cgctgcgctc agtttcagat cgccaaattc tgaattacaa aaacgttgct catatttggt
     9781 gacccgatta ccaaaaccgc tcactgtgcg tagataatga cgtttgacca gattatccag
     9841 ctgttcctgc acttcggatt cactcagatt catgaccggt tcacggttcg ttttctgatt
     9901 acaggccgtg actacaccat tgactgagag cgggtattgc tccggcgtgg tcacctgctt
     9961 ttccagcaag caaccaatca cgcgagcttc aagggcagta agttgatatt tcatgagatt
    10021 ctccttagcg gccgggcgtc cagtctgggg tagttaatgc cgtcagtacg tgatcgcgcc
    10081 attgtccatc aatcaacaga tagtctttcg catagccttc tttttcaaaa cccagtcgcg
    10141 ccagtaaatc accgctgcgt ttattgtgcg gcatataatt agccataatg cgatgaatat
    10201 gttgggtgcg ctgcatataa cgaatggctg cggtcagggc ttcaaacatg agtcctttgc
    10261 cctgccattt ttgcccaatc gaataaccga gatagcaggc atgaaaagag ccacgaacaa
    10321 cattggaaaa attggcaacg ccaataatct ctttttcgtc cgggtcgaat aagccaaagt
    10381 agaaagctga accttgttta tgaaattcgt taatcatccc cagcctggcc tgccagcctg
    10441 atggataaca gtggctttcg tcgcgcactg gctcccaggg cttgaggaaa tggcgattct
    10501 ctgcgtaata atccgcaaga cgccaggcat cacgatcatg caccagacgc acgaccagtc
    10561 ggtctgtggt taagcgcact tttggcacgt tactgcgata gccaaacatt ctatacctac
    10621 tccttcccgt aacgtcttta atacgctgta agtattacta tacctgcgca accagaccct
    10681 gtgaaaagtc caaaatgcca ttttctgcat tattcacata tttgataaaa actctctatc
    10741 aaagcgacgt tttgataaaa aaatattgtc ccctaagtga tgggaaaagt cttagcggca
    10801 atgcagaata gaaagtgctc atcttttatt tcccgggagg ggaaatgtcc cgcgtatcgc
    10861 aggcgaggaa cctgggtaaa tatttcctgc tcatcgataa tatgctggtc gtgctggggt
    10921 tctttgttgt cttcccgctg atctctatcc gcttcgttga tcaaatgggc tgggccgccg
    10981 tcatggtcgg tattgctctc ggtctacgcc agtttattca gcaaggtctg ggtattttcg
    11041 gcggcgcaat tgccgaccgc tttggtgcca aaccgatgat tgttaccggt atgctgatgc
    11101 gcgccgccgg attcgccaca atgggtatcg cccacgaacc gtggctattg tggttttcat
    11161 gcctgctctc gggactcggc ggcacgttgt ttgatccgcc gcgttcggcg ctggtggtga
    11221 aactaatccg tccacaacaa cgtggtcgtt ttttctcgct gttgatgatg caggacagtg
    11281 ccggtgcggt cattggcgca ttgttgggga gctggctgtt gcaatacgac tttcgcctgg
    11341 tctgcgccac aggggccgtt ttgtttgtgc tgtgtgcggc gttcaatgcg tggttgttac
    11401 cggcatggaa actctccacc gtacgcacgc ccgttcgcga aggcatgacc cgcgtgatgc
    11461 gtgacaagcg ttttgtcacc tatgtcctga cgctggcggg ttactacatg ctggctgtac
    11521 aagtgatgct gatgctgcca attatggtca acgacgtggc tggcgcaccc tctgccgtta
    11581 aatggatgta tgccattgaa gcgtgtctgt cgttaacgtt gctctatcct atcgcccgct
    11641 ggagtgaaaa gcattttcgt ctggaacacc ggttgatggc tgggctgttg ataatgtcat
    11701 taagcatgat gccggtgggc atggtcagcg gcctgcaaca acttttcacc ctgatttgtc
    11761 tgttttatat cgggtcgatc attgccgagc ctgcgcgtga aaccttaagt gcttcgctgg
    11821 cggacgcaag agctcgcggc agctatatgg ggtttagccg tctgggtctg gcgattggcg
    11881 gcgctattgg ttatatcggt ggcggctggc tgtttgacct gggtaaatcg gcgcaccagc
    11941 cagagcttcc gtggatgatg ctgggcatta ttggcatctt cactttcctt gcgctgggtt
    12001 ggcagtttag ccagaaacgc gccgcgcgtc gtttgcttga acgcgacgcc tgatttgata
    12061 ctcccccctg actcctccat actgaaagtg aactgacagc caaatggagg agtcacgtga
    12121 agctatacat ttacgatcac tgcccttact gcctcaaagc ccgcatgatt ttcggcctga
    12181 aaaatatccc cgtcgaatta catgttctgc tcaacgacga cgcagaaaca cccacccgga
    12241 tggtcggtca aaaacaggtt cccattctgc aaaaagatga cagccgctat atgccagaaa
    12301 gcatggacat cgttcactat gtcgataaac tcgacggcaa accgttactg accggcaaac
    12361 gttcccctgc cattgaagag tggctgcgca aggtcaatgg ctacgccaac aaactgctgt
    12421 tgccgcgttt tgccaaatcg gcatttgatg agttttctac tcccgccgca cgcaaatatt
    12481 tcgtcgacaa gaaagaggcc agcgcgggta attttgccga cctgctggcc cactctgacg
    12541 gtctgattaa gaatatcagc gatgatttac gtgcgctgga taaactgatc gtcaaaccga
    12601 acgccgtgaa tggcgaactt tcagaagatg atattcagct atttccgcta ctacgtaatc
    12661 tgacgctggt agccggaatc aactggccaa gccgcgttgc tgattaccgc gataatatgg
    12721 cgaaacagac acaaatcaat ttgttatcat caatggcgat ttaatcctga cccgccgcgg
    12781 cagcctgtat tcgcgggttg ccgccgtttt cagttttttc tgctggtgag tttatcgctg
    12841 gtgacacatg ctgcgttgtg tcaggatatt gacgcagatt tcatgtcgag gactaccatg
    12901 aacaagtttt tatttgccgc tgcattgatc gtcagtggcc tgctcgttgg ctgtaatcaa
    12961 ctcacccaat acaccatcac cgaacaagaa attaaccagt cgcttgcgaa acataataat
    13021 ttctcaaaag atatcggttt acccggcgtg gctgacgccc atattgttct gacaaacctg
    13081 accagccaaa ttggtcgcga agagccgaat aaggttaccc taaccggaga cgccaatctg
    13141 gacatgaact ccctgttcgg tagccagaaa gcgaccatga aactgaagct gaaagcgctg
    13201 cccgtgtttg ataaagaaaa aggcgcgatc ttcctgaaag agatggaagt ggtcgatgcg
    13261 acggtacaac cggaaaaaat gcaaacggtg atgcaaacgt tgcttcccta tttgaaccag
    13321 gcattacgca attactttaa ccagcaacct gcttacgtcc tgcgcgaaga tggcagccag
    13381 ggcgaagcaa tggcgaaaaa actggcgaaa ggcattgaag tgaagccagg cgaaattgtc
    13441 attccattta ctgattaatc acgagggcgc attcgcgccc tttatttttc gtgcaaagga
    13501 aaacgtttcc gcttatcctt tgtgtccggc aaaaacatcc cttcagccgg agcatagaga
    13561 ttaatgactg caccatccca ggtattaaag atccgccgcc cagacgactg gcaccttcac
    13621 ctccgcgatg gcgacatgtt aaaaactgtc gtgccgtata ccagcgaaat ttatggacgg
    13681 gctatcgtaa tgcccaatct ggctccgccc gtgaccacag ttgaggctgc cgtggcgtat
    13741 cgccaacgta ttcttgatgc cgtacctgcc gggcacaatt tcaccccatt gatgacctgt
    13801 tatttaacag attcgctgga tcctaatgag ctggagcgcg gatttaacga aggcgtgttc
    13861 accgctgcaa aactttatcc ggcaaacgca accactaact ccagccacgg cgtgacgtca
    13921 attgacgcaa tcatgccggt acttgagcgc atggaaaaaa tcggtatgcc gctactggtg
    13981 catggtgaag tgacacatgc agatatcgac atttttgatc gtgaagcgcg ctttatagaa
    14041 agcgtgatgg aacctctgcg ccagcgcctg actgcgctga aagtcgtttt tgagcacatc
    14101 accaccaaag atgctgccga ctatgtccgt gacggaaatg aacggctggc tgccaccatc
    14161 actccgcagc atctgatgtt taaccgcaac catatgctgg ttggtggcgt gcgtccgcac
    14221 ctgtattgtt tacccatcct caaacgcaat attcaccaac aggcattgcg tgaactggtc
    14281 gccagcggtt ttaatcgagt attcctcggt acggattctg cgccacatgc acgtcatcgc
    14341 aaagagagca gttgcggctg cgcgggctgc ttcaacgccc caaccgcgct gggcagttac
    14401 gctaccgtct ttgaagagat gaatgctctg cagcactttg aagcattctg ttctgtaaac
    14461 ggcccgcagt tctatggctt gccggtcaac gacacattca tcgaactggt acgtgaagag
    14521 caacaggttg ctgaaagcat cgcactgact gatgacaccc tggtgccatt cctcgccggg
    14581 gaaacggtac gctggtccgt taaacaataa aaatcgtagc ttcctgttgt cattaggtta
    14641 ttttgcctgt ataaataacc agtatattca acagggggct attatgcgaa ttgaagtcac
    14701 catagcgaaa acttctccat tgccagctgg ggctattgac gccctggctg gcgaactttc
    14761 ccgccgtatt cagtatgcgt ttcctgataa tgaaggccac gtatcggtac gttatgccgc
    14821 agcgaataat ttatcggtta ttggcgcaac aaaagaagat aaacagcgca ttagcgaaat
    14881 tctccaggaa acgtgggaaa gcgccgatga ctggtttgtc agcgaataat atgcagtgat
    14941 ttttgttttt gccgggtcgc cccggctttt ttattttact tacaaatttt gagtacaaaa
    15001 tttctacact tcctcaaaat aattgcacaa tatgctaaaa aaatatgaat gacatgctgt
    15061 tttattgttc atataaatct taaaagcaga aatttatctg ttcccccctt taaaaattcg
    15121 gtgaataccc ttacttattg gtatactgaa gttgcttcag ataaaaacgc attgaacctc
    15181 gaataacgtt gtctagtaac acgaattagg gggccatgat ggaaaaaaat aatgaagtca
    15241 ttcagactca tccgctcgta gggtgggaca tcagcaccgt tgatagctat gatgcgctga
    15301 tgttgcgttt gcactaccag accccaaata agtccgagca ggaagggact gaagttggtc
    15361 agacgctctg gttaaccact gatgttgcca gacaatttat ttcgatatta gaagcaggaa
    15421 tcgccaaaat tgaatccggt gatttcccgg taaacgagta tcggcgtcat taacatgaca
    15481 tgaccatcca cccaaaaggc acctcaccgg tgcctttacc atttctgggt tgtatatctg
    15541 cattcagtta attactctgc ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc
    15601 tcatcattat tggcagcggt tccgtaggcg ctgccgccgg gtattatgca acccgcgccg
    15661 gtttaaacgt gctaatgacc gacgcccata tgccaccgca tcaacacggc agccaccatg
    15721 gcgatacgcg attaattcgc catgcttatg gtgaaggcga aaagtatgtc ccgctggtcc
    15781 tccgcgcgca aacgctgtgg gatgaacttt cccgccacaa cgaagatgat cccatttttg
    15841 tacgctctgg tgtcattaac ctcggcccgg ctgactccgc atttctcgcc aacgtcgccc
    15901 acagcgccga acagtggcaa ctcaacgttg aaaagctcga tgcgcaaggg attatggccc
    15961 gctggccaga aatacgcgtc ccggacaact acatcggctt atttgagact gattccggtt
    16021 ttttgcgcag cgaactggcg attaaaacct ggatccaact ggcgaaggaa gcgggctgtg
    16081 cgcaactgtt caactgcccg gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg
    16141 aaaccgttga cggtgagtat caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa
    16201 aagacctgct cccggagctg cctgtccagc ccgtacgtaa agtatttgcc tggtatcagg
    16261 ccgatggccg ctatagcgtg aagaataaat tcccggcgtt taccggtgaa ctgcccaatg
    16321 gcgatcaata ttatggtttt ccggcagaaa acgacgcgtt gaagattggc aaacataacg
    16381 gaggccaggt tatccattca gcggatgaac gtgttccgtt tgcggaagtg gtcagcgatg
    16441 gttcggaagc cttcccgttc ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg
    16501 gcgctgcctg cacctatgat aattcgcctg acgaagattt tattatcgat accctacccg
    16561 gccacgataa tacactgctc attaccggcc tgagtgggca cggttttaaa tttgcgtcag
    16621 ttttagggga aatagctgcc gattttgcgc aagacaaaaa aagcgatttt gatttgacgc
    16681 cattcaggct ttcccgcttc caataatcat cattacggcc tccagaatga ggccgttttt
    16741 acaggagtat ttaatgcgtc ggctgttgca ctatctcatc aataatattc gcgagcatct
    16801 gatgctttat ctttttctct ggggattgtt ggcaattatg gatctgatct atgtgtttta
    16861 tttttagttt tccgcactga cataaactga caaatattta atctcccttt catttggcgt
    16921 gaaaaattga atgagattat tcctgcttct acagcactca ccagacatct ctgatgctga
    16981 acccactaaa cttatgaaga ttctttcatt attaattcaa aattattgaa ataaaaataa
    17041 atatcattca atttgttatt tttgtgttgc aattcattct ccgacacgcc attttctttc
    17101 tctcgcaaat tcgtattgga tgcacttatg tcattcacaa atacccctga acgctatggt
    17161 gttatatctg cggccttcca ttggctaagc gcaattattg tctacggcat gtttgcttta
    17221 ggtctgtgga tggtcacgct cagttattac gatggctggt atcacaaagc acccgaactg
    17281 cataaaagca tcggtatttt gttaatgatg gggctggtta ttcgcgttct gtggcgtgtc
    17341 atatctccac cgccgggtcc gctgccgagt tattcgccca tgactcgtct tgccgcaaag
    17401 gctggacatc tcgcattgta cctattgctg ttcgccatcg gcatcagcgg ctatctgatc
    17461 tcaactgccg atggtaaacc gatcagcgtt tttggctggt ttgacgtccc cgcgaccctt
    17521 gccgacgctg gcgcacaggc agactttgcc ggtgccctgc atttttggct cgcgtggagc
    17581 gtcgtagtac tgtccgttat gcacggattt atggccctga agcatcattt catcgataaa
    17641 gacgacactc tgaagcgcat gctgggaaag tcgtcatctg actatggagt ataaaaaatg
    17701 aaaaaaagcc tgcttggttt aaccttcgcg tccctgatgt tctctgccgg ttcagcggtt
    17761 gccgccgatt acaaaattga caaagaaggt cagcacgcct ttgttaattt ccgcatccag
    17821 caccttggct atagctggtt atacggcacc tttaaagatt tcgacggtac ttttaccttt
    17881 gacgaaaaaa atccggctgc cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat
    17941 actaatcacg ccgaacgcga taaacatctt cgcagtgcag atttcctcaa taccgcaaaa
    18001 tatccacagg caacattcac ctccaccagc gtgaagaaag acggtgacga actggatatt
    18061 accggcgatc tgacgctgaa tggcgtaacc aaacccgtca cgctggaagc gaaattaatt
    18121 ggtcagggcg acgacccatg gggtggtaaa cgtgcaggct tcgaggccga aggcaaaatt
    18181 aagctcaagg acttcaatat caagacagat ttaggtccag cttctcagga agtggatctg
    18241 attatttcag tggaaggcgt acagcagaag taagcgtgaa atgttgccgg atggcacgca
    18301 tccggcaatg atatttattc tgttggatca ggaatgccca gtgttgtatt cagacgtcca
    18361 cgagacttat taaagatctt attgccattt tcacgtcctg cccgacggcg tcgctgttct
    18421 tctggcggta acgcgctttc ttcgcagcaa atctcactac aacaaccttt gtatttttcc
    18481 gcgcatactg gacactgaat aaacagcagg tggcagccat catttttaca gttggtatgg
    18541 ctgtcgcacg gcgcgccgca ctggtggcaa tgcgcgataa tctcatccga tatacgttcg
    18601 cccatccgct cgtcaaaaac aaaatttttg ccaataaaac gcaccggcaa gccctgctcg
    18661 cgcgccttac gggcgtattc aataattccg ccctcgatat gccagacttt gttgaatccg
    18721 ttatgtttca tccaggcact ggccttttca caacgaatgc cgccggtgca gtacatgacg
    18781 atttttttat ctttatgtgc ctgcatcatc tcgactgctt ttggcagctg ctcacggaag
    18841 gtatctgccg gaatttcgag cgcgttttca aagtgcccca cttcatactc atagtggtta
    18901 cgcatgtcga taaatagtgc atcgggatcg tcaagcatgg cgttcacttc cgccgcttgc
    18961 agatactcac caacattgct ggcatcaaag tgaggatcgt caataccgtc ggcaacaatg
    19021 cgatcgcgta ccttcatgcg cagtacccag aaggatttcc cgtcatcatc caacgcgata
    19081 ttcaggcgta aaccctctaa agccgggtcg aaggcataga gctgcgcgcg aaatgtttca
    19141 acattgctcg caggtacgct gatttgcgcg ttaatgccct catgcgccag atacactcgc
    19201 ccaaaaacat tcagcgcggt aaacagctga tataaagcgt cacgggtcgc cttaggatcg
    19261 gcgatgtgga aatacttgta aaacgaaatg gtggttcgcg gttcgctctc agccaacatt
    19321 ttggctttta gcgcgtcgtt ggaaatgcgg ttgtgtaaca ctggcatggt gtacggttcc
    19381 tgcgagatgg gaaagtaaaa atccgcggca tgatatagca attatcgata attaacatcc
    19441 acacatttta cgctacattt gcgcattaaa aattatttgt tatttacaag cgcggcaatt
    19501 tcgcccagtc ttcagccaca aattttggtt gcgggcgaaa aaatgcgaca atacatacaa
    19561 ttgcccgaat aggttgaaaa acaggattga tatgacgaat ctacccaagt tctccaccgc
    19621 actgcttcat ccgcgttatt ggttaacctg gttgggtatt ggcgtacttt ggttagtcgt
    19681 gcaattgccc tacccggtta tctaccgcct cggttgtgga ttaggaaaac tggcgttacg
    19741 ttttatgaaa cgacgcgcaa aaattgtgca tcgcaacctg gaactgtgct tcccggaaat
    19801 gagcgaacaa gaacgccgta aaatggtggt gaagaatttc gaatccgttg gcatgggcct
    19861 gatggaaacc ggcatggcgt ggttctggcc ggaccgccga atcgcccgct ggactgaagt
    19921 gatcggcatg gaacacattc gtgacgtgca ggcgcaaaaa cgcggcatcc tgttagttgg
    19981 catccatttt ctgacactgg aactgggcgc gcggcagttt ggtatgcagg aaccggggat
    20041 tggcgtttac cgcccgaacg ataatccact gattgactgg ctacaaacct ggggccgttt
    20101 gcgctcaaat aaatcgatgc tcgaccgcaa agatttaaaa ggcatgatta aagccctgaa
    20161 aaaaggcgaa gtggtctggt acgcaccgga tcatgattac ggcccgcgct caagcgtttt
    20221 cgtcccgtta tttgccgttg agcaggctgc gaccacgacc ggaacctgga tgctggcacg
    20281 gatgtccggc gcatgtctgg tgcccttcgt tccacgccgt aagccagatg gcaaagggta
    20341 tcaattgatt atgctgccgc cagagtgttc tccgccactg gatgatgccg aaactaccgc
    20401 cgcgtggatg aacaaagtgg tcgaaaaatg catcatgatg gcaccagagc agtatatgtg
    20461 gttacaccgt cgctttaaaa cacgcccgga aggcgttcct tcacgctatt aaatctcccc
    20521 tgccggatgc ttcagaatgg catccggcat taccacagca aatccccctg atttagcgat
    20581 aaaagctctc tggattgcgc cccctggaag tcgggcgcat aattagtgtg cttatctttt
    20641 cttcttatgt tcaccgcgcc tggcgcacca acagcggatt gctatgtcac cctgtgaaaa
    20701 tgacacccct ataaactgga aacgaaacct gatcgtcgcc tggctaggct gttttcttac
    20761 cggtgccgcc ttcagtctgg taatgccctt cttacccctc tacgttgagc agcttggcgt
    20821 taccggtcac tccgccctga atatgtggtc cggtattgtc ttcagcatta catttttatt
    20881 ttcggccatc gcctcaccgt tttggggtgg actcgccgac cgtaaaggcc gaaaactcat
    20941 gctattacgc tctgccctcg gcatgggcat cgtgatggtg ttgatgggac tggcacaaaa
    21001 tatctggcag tttttgatcc tacgggcgct tcttgggtta cttggcggat ttgtccccaa
    21061 cgctaatgct cttatcgcca cacaagtacc gcgtaataaa agcggctggg cgctgggtac
    21121 gctctccaca ggcggcgtta gtggtgcgtt gctcggccca atggctggcg gcctgctcgc
    21181 cgatagctac ggcttacgtc cggtattctt tattaccgcc agtgtgctca tactctgctt
    21241 tttcgtcacc ctgttctgca tcagagaaaa attccagccg gtcagcaaaa aagagatgct
    21301 gcacatgcgg gaagtggtga catcacttaa aaacccgaaa ctggtactca gcctgtttgt
    21361 caccacgtta atcatccagg tggcgacagg ctcaattgcc cccattctga cgctgtatgt
    21421 ccgcgaactg gcgggtaacg tcagtaacgt cgcctttatc agtggcatga tcgcctcggt
    21481 gccaggcgtg gcggctctgc taagcgcacc acgactcggc aaacttggcg atcgaatcgg
    21541 acccgaaaag atcctgatta cagcgctgat cttttctgta ctgctgttga tcccaatgtc
    21601 ttacgttcag acgccattgc aacttgggat tttacgtttt ttgctcggtg ccgccgatgg
    21661 tgcactactc cccgccgtac agacactgtt ggtttacaac tcgagcaacc agatcgccgg
    21721 gcgtatcttc agctataacc aatcgtttcg tgatattggc aacgttaccg gaccattgat
    21781 gggagcagcg atttcagcga actacggttt cagagcggta tttctcgtca ccgctggcgt
    21841 agtgttattc aacgcagtct attcatggaa cagtctacgt cgtcgtcgaa taccccaggt
    21901 atcgaactga tttttcgcct ttcatacttg caaaagcgga gaatcagcta tccttttccc
    21961 tgaaacctca tcaactcaaa gggagaatcg tgatgaccat gtacgcaacg cttgaagaag
    22021 ccattgacgc tgcacgcgaa gaatttcttg cagacaaccc cggcatcgac gccgaagatg
    22081 cgaatgtgca acagttcaat gcccaaaaat acgttttgca ggacggcgac atcatgtggc
    22141 aagttgagtt ttttgccgac gaaggggaag aaggtgaatg tttacctatg cttagcggtg
    22201 aagccgcgca aagtgttttt gatggcgact atgatgagat agagatacgc caggagtggc
    22261 aggaagagaa tacattacat gaatgggacg agggggaatt tcagcttgag ccaccgctgg
    22321 ataccgagga aggacgcgca gcagctgatg agtgggatga acgttaatca ctcatacggg
    22381 ccatgatgaa tgtcgatcgg cagcagtaaa gtatcaaaaa ccagagagaa tggcagatca
    22441 aggatcgtga cataacgcca ggcggagtca cgcacatccc attgcacacc gggataatat
    22501 tggttgccat gcccctgccc cggtatagtg cgactaataa tgctgccaca gccactcagc
    22561 aaggtcacca tgatgctcac cacaattaaa cgcatttgat acctctttcg taaaaaaata
    22621 ccggcataac gccggtattt ttattggctc gttgtttttg ccggatgcgg cgtgaacgcc
    22681 ttatccgacc cacaaaatct tgcaaaatca ataaattgca ggaacgatgt aggcctgata
    22741 agcgtagcgc atcaggcaac tacgttttta ttgcgaagcc gcatccggtt tacgcaatgc
    22801 cagtggattg agctttatcc cttcgtaata actcacccat gaagaatatc tctccgggga
    22861 attccagacg cggaaatgca gacgggccat cgtcaccgga tcgcttagca gcaccaggcg
    22921 acgatcgcga ttcagcttct ctggcgtctc gttcagcgcc tgttcaacgt ggcggtcacg
    22981 ggcgatttcc agcaccttac tggcgcggtg acgcgcggtc gccattgcgg ttgccagagc
    23041 gttaaatgac gggttaaaca ctgcgtgcat aaagccatca tcaagggagc gttgacgatt
    23101 catctcaagg aaccgatcgg tatcaaccag cacctgcggc ggcgaatact cttccgggat
    23161 caggaacagt ttccagcgtt tggtgcgcag accaacggtg gcacggctgg aaatcaccga
    23221 aacaaacggt gacaggatca acgagaagac aatcggtgcc agccagaaca ggaaacgcag
    23281 atccaaccac gccataccaa cagcccacac taaccctaac agcagctgtg agccgtggcg
    23341 tttgaacgct tcaccccagg aagtggagtc atcatcacgc tgcggtgaat tccacaccac
    23401 ttcccagcca aggaacgcgc tgacgacgaa gaccgtatgg aacagcatgc gtaccggagc
    23461 cagcagcacg gaaaacagca cttccagcag caacgataat gtaacgcgcc agaagccgcc
    23521 gtattctttc gttcctttgc accagataag caaaatgctc aataacttcg gcaggaacaa
    23581 cagcaccatg gtcgaagcaa aaagtgcaat cgccagctca ggacgccact gcggccacac
    23641 cgggaacaac tgccgtggtt gcaggaagta ttgcggttcg gtcaacgcat gtactacctg
    23701 caatgcagta gagagcgcga ggaacataaa ccacagcgga gcggagagat aagacatcac
    23761 gcccgtcagg aataccgcac ggtgaaccgg gtgcataccc ttaaccagga acagacggaa
    23821 gttcatcagg ttaccgtggc accagcggcg gtcacgtttt agctcatcaa gcaagttagg
    23881 cggtaattct tcataagaac ccgggagatc gtaagcaatc cagacccccc aacctgcacg
    23941 gcgcatcaac gccgcttcta caaagtcatg tgacaggatt gaaccggcaa aggaaccttc
    24001 gcccggcagc ggagccagtg cgcagtgctc gataaacggt ttcacgcgga taatcgcgtt
    24061 atgtccccag tagtgcgact cgccaagttg ccagaagtgc aaaccggctg taaacagtgg
    24121 cccatacacg cgggtcgcga actgctgaca gcgcgcatac agcgtatcca taccggacgc
    24181 tttcggcgac gactgaatga tcccggcgtt cgggttggct tccatcaggc gcaccagccc
    24241 gcacaaacaa tcaccggtca ttaccgagtc agcatccagc accaccatgt agctgtactg
    24301 gctgccccag cgacggcaga agtcatcgat attaccgctt ttacgcttca cgcggcgacg
    24361 gcggcggcga tagaaaatct gaccttcgcc accgacttca gcgataagct ccatccaggc
    24421 tttttgctct gcgacgcaga tatccgggtt ataactgtca ctaagaatgt agacatcaaa
    24481 gtgtttggca ttcccggtgg cttttactga ttcccacgtt gcacgcaggc cagcaaaaac
    24541 acggttcacg tcttcgttac agataggcat gatcaacgcc gtgcgatgct ccgggtttaa
    24601 tggttcatcg ccaactgttg acgcagatat actgtattta tcgcgaccaa taagcagttg
    24661 caggaagccc attaacgccg tccagaatcc ggcggacacc caacagaaca gtaccgcaaa
    24721 gaggatcagg ataccggttt gcagcatata aggcagaagc tgcataaagg aaacccacaa
    24781 atcctgacca accatatcca taggattaat cagcgcccaa ccctgataag gaagaatggt
    24841 cttcatatac caggtcgcga cgacagtttg cgcgagcgtc aggatcaaca gaatgtaacg
    24901 gcggatggta ccgacggtac gccacttttg ctcgctctcc tgctcttctt tggtcaaacg
    24961 agccagatag cgcggcgtga catcgcgtcc acgcaggcga tcccagaaac ggcctaccgg
    25021 gttggtacgc cacgggtcgg gaaacatcga ggagcgtttt gcttctggca tcgccttcag
    25081 ctgatcgcgc ccttcgtcgt ctttaattaa ctgtccatca gcaagtgaat ctggccaggc
    25141 ttgttccaga cgcgccttta ccgagccttg cggggaatca tcctcccgcg cccaggtgcg
    25201 gtgttcggca tccagcgcct gatgaacggc gcggatatca gtcttcggca atgccgcttt
    25261 ctcgcttgcg gcgatgggca ttgcgtcaat gtactcagtt gtcttattca ttggcaggta
    25321 actggtagct ccaggtttca ctcaacgtct gatcggcatt caccagcgca gcacgcattt
    25381 cagtggtttt cttggcatct ttcactttca cacgcatcac cagacgccag cctttggtaa
    25441 ccgggttata gcgcaccgtg ctttcaacta tctcaccatt atcaccaatg ctggtttgcg
    25501 ctgtgaccgg ggtatcctct ggcagttttt tcatctctgc gccggtaaaa tcgaccacaa
    25561 aggcgatagt accgtcaggc tggcgaatca ggttcgactg cttcacatcc cccgttgaac
    25621 gacgcgtttg ttgcacccat gcgttatctg gcgcatgcag tttgtcttca tcacggctga
    25681 aggtgatggt gtatttaaag ttcatctctt tacccggctc cggcagctga tccggcgtcc
    25741 agtaagcgac gatgttatcg ttggtttcat cgttggttgg aatttctacc agctcaacgc
    25801 tgcctttacc ccactcccct ttcggagtca cccatgcgct tggacgaaga tcgtaacgat
    25861 catcgagatc ttcaaagcgg gagaaatcac gaccgcgctg caacagaccg aagccttgcg
    25921 ggttttccat agagaagctg ctgaccgcta aatgtttcgg gttattcaac ggacgccaga
    25981 tccattcgcc attaccggca tggatagaga gaccgttaga gtcgtgcaac tccggacgat
    26041 agttatttgc aggcgacggt tggttcggcc caaacaggaa catactggtt aacggtgcaa
    26101 cccccagttt gccgacttta tcgcgcagat agattttcga ctgcacatcc acaaccgtgt
    26161 cacgccctgg cataaccacg aatttgtaag cacctgtcgc gcgcggcgag tcaagcaatg
    26221 cataaatggt taaacgttta tcagtcggtt ttggacgctc gatccagaac tctttgaagc
    26281 gtggaaattc ttcacccgat ggcaaggcgg tatcaattgc caggccgcgg gcagaaaggc
    26341 cataaacctg acctgcacca atcacgcgga aatagctggc cccgagcatg ctgacgattt
    26401 catcgttttt atctttgctg ttgatcgggt aaagcacttt aaaaccggca aaaccaaggt
    26461 cttttaccgt gtctttgtca tgctgaacat cgccgaaagt gaaataatcc gggctgtatt
    26521 tgattcgttt gactgcggtg gcagtcactt catttatttt gaccggggta tcgaagtaca
    26581 taccctgatg gtagaactcg agtttgaatg gggtcttcag attgttccag tacgctttgt
    26641 catgattaaa ctggatctgc tgatagtccg cgtatttcat atcgcggaaa acggagggca
    26701 agttgctttt gggcgcctca tagcctttcc cggctaagga ttgagcttgc tttgcgacat
    26761 catcaatact gaaagcccag cttgaagatg tatacagggt taacattact gcagcactca
    26821 accaacgcat tttcatcatt tgtagtttat gtttcataat tagtaagcac ttcccccttt
    26881 gtgtgcttat atcgatccga tctattttaa tggataacca gccattccga caacagaatc
    26941 ccggtatcgt tcagcacgct ggctcatgga ttaagcatct gattgcaacc gttttcactt
    27001 tccgtgcttc tcctaaagac agaagcatcg acttcgtgta gggtttgcca gcaattttat
    27061 ttatcaaccg tcttacagac gcttccattc tggttagtgg tagcgatgat gctatgcata
    27121 aaaaccttga agcctgcgcc tggcgcggtc aggtcggtgt cgctatggca acaagcctaa
    27181 aggcataacc cgacagggat aagacgaaaa atccgagatt agttaaccat atatgaatcc
    27241 agtacccgcg caacgtgaat atttcctcga ctccatccgc gcctggctga tgttgttagg
    27301 gatccctttt catatttctt taatctattc gagccataca tggcatgtga atagcgccga
    27361 accatcattg tggctgaccc tttttaatga cttcatccac tcgttccgca tgcaggtatt
    27421 tttcgttata tccggctact tttcctacat gcttttttta cgctatccct tgaaaaaatg
    27481 gtggaaagta cgtgtcgaac gtgtaggtat cccgatgtta acagccatcc ccctactgac
    27541 attaccgcaa tttattatgc tgcaatatgt caaaggaaaa gcggaaagtt ggcctgggct
    27601 gtcattgtat gacaaatata atacgttggc ctgggaatta atatcacacc tgtggttttt
    27661 actggtgtta gtagtcatga cgacgctgtg cgtatggata tttaagcgca tcagaaataa
    27721 tttagaaaat tctgataaaa cgaataaaaa attctcgatg gtaaaactat cggtgatttt
    27781 tttatgcctc ggcatcggtt atgcggtaat aagaagaacg atttttattg tgtatccgcc
    27841 cattctgagt aatggcatgt tcaattttat tgtcatgcaa acgctatttt atttaccgtt
    27901 ctttatcctc ggcgcactgg ctttcatttt ccctcatctt aaagccttgt ttaccacgcc
    27961 gtctcgtggc tgtaccctcg ctgcagcatt ggcgtttgtc gcttatttac tcaaccagcg
    28021 ctatggcagt ggcgatgcct ggatgtacga aaccgagtca gtgatcacca tggtcctcgg
    28081 tctgtggatg gtgaatgtgg tcttctcctt tggccaccgt ttgcttaact tccagtcagc
    28141 gcgggtgact tattttgtta acgcatcgct atttatctat ctggttcacc acccgttaac
    28201 gctgtttttc ggcgcataca ttacaccgca catcacctcc aactggcttg gttttctctg
    28261 tggcctgata ttcgtagtag ggattgcgat aattctgtat gaaattcatt tacgcatccc
    28321 gttactgaag tttttgtttt ctggtaaacc ggttgttaag cgtgagaacg ataaagcacc
    28381 agcccgttaa gccacattta caataaccat tccacgggca atatcgacgc cagtctgacc
    28441 ataacccgct tccagaaact ggtggcgggt tcttttttga gaataatctc tttctttgca
    28501 tgacgatcaa cccagttgat ccgtccccac ctgtccagac ggagctgcca ggccgcatca
    28561 tactggctct gaataaagcg tttatcaatt aactgtgcca gcgtttcgct ctctatcacg
    28621 aagcccattt cagtattgag caatgttgaa cgcggatcga aattgaaaga gccgataaac
    28681 accgttttac catcgatgct aaaggtttta gcatgcaggc tggctccgga attaccggtt
    28741 atgccgcgat cgtgtaacgt actactttgt tcacgcgtcg gcttgagttc atataattcc
    28801 acgccatagc ggagcaattt tttgcgccag cgcgcgtatc cggcatggac gacagcaaca
    28861 tcgttagcgg caagagaatt ggttaggatc gcaatcttta ccccttttct caccatccgt
    28921 aagagttgcg ccacacctgc gcgtgtcggt acaaaatagg aagagataat atcgatgcgt
    28981 tcactgggtg agcccatgat atcgaacagg cgctgcggta acagtgaatg ccgttttgcc
    29041 ttgccctccc ctttcgccgg atcatcactt aataaacgtg tcttcgccca gataagcggc
    29101 aatgttccat caaccagatg atttataaat ggactggatt ccattttgcg taaataacga
    29161 tgcgtcatgg catcgttatg ccaggaggcg ggtaactcga tgcgatccgc catttcaccc
    29221 tccgggacat ccagcacctg ctgtaagggt gaaaccgatt tgcaatacca gtagcgggcg
    29281 aaatcatcgg caacgtcctc taccacgggt cctattgcca tgacatctaa atccgaaaaa
    29341 agtggctcct cccctgctcc aaaataggca tcaccaatat ttcgtcctcc caccagggtc
    29401 accacgccat cgacagtgaa acttttattg tgcatacggc gattaagacg ggaaaagtcg
    29461 gtgatataac caagcggacg cagcaagcga aacgagaaag gattaaaaag ccggacttca
    29521 atgcgtggat gactgtcaag caagcgtaaa atgtcgtcaa gtccgggcgt attgttgtcg
    29581 tccagcaaca aacggacgcg aacgccacgc tttgctgcgg ctaacagggc ggaaaacagt
    29641 aaccgacccg acatatcgtc ctgccagatg taatactgaa catcgagcgt atgctctgcc
    29701 atttcggcca gacgataccg ggcggcaaac gcatccagac ttttctccag agggaaaaga
    29761 ccacactgac cgggatgttg cgaacacagt ggcagcaccg cgctcgccag ccggggcaaa
    29821 tcattcatct ccttgttggg taaggagtct ttcgtagagg tgggcgtttt cttcatcata
    29881 acagacaaag tatacctgtt cgggtaaagc gtgacgggta ataaattctg aaacggtttt
    29941 aactgcgatt tcagccgctg ccgcacgagg gtaaccataa accccagtac tgattgcagg
    30001 aaaagccact gacgtatagc tgtttgccgc caccagtcgt aggctattga gataggcatc
    30061 ctgcaaaagc tggtcttcgt tttgttcacc accacgccag actggcccga cggtgtgcac
    30121 tacggcttta gcgggaagag cgcctgcaag cgtaataacg gcatgccccg tagggcaatc
    30181 gccctgctgt tgcctgactt ttaaacaggc atccagcagg gccggacccg ctgcgcgatg
    30241 aatggcccca tcgacgccgc cgcctcccat taatgacgga ttagccgcat tcacaatcac
    30301 atcaacggcc agtttggtaa tatcaccctg cacaacatga atacgcgttt tcataacgcc
    30361 tccttacacc ttcttgtttc ttaagtgtac agcaggcttt ttggttaaat cgcatttttc
    30421 tgctcagcaa cgtgcgttgt tcagaaactt agttatattc gatgttcatt acagccagct
    30481 ttttctgttt atcaagatac tgcactttta ctgaggctat ttgcgcgttt ttaacattac
    30541 cgctcattaa cgctttgctg gaataggttt tgcccggaac agaaccattc tgtgggcagt
    30601 tcatttctac gggtgatgac tgggtgctga cgtcacatgc cggttccaca atttggccat
    30661 aaaaatggat caccccccct tgcgcggtgc ttcctgcgat ggcaataaaa ggaaaaagca
    30721 aactgccgag cataagagag tttaaaaaag gtctgaacat ttattctatt tcctcaatga
    30781 ttagtcatcc ttgagggttg tgttatccat actttcaggt tggctgattc gcagcagacc
    30841 attctctcca gattcatctt atgctcgata tttcaacaaa ttaagacttt tctgaagagg
    30901 gcggccattg ttgtgataaa tgaagtgact gtccatcaga aacagtaaca actattttca
    30961 cccgatcttc cggggaaata tttaaactca acttcgtcaa agcaataggt tgattagcag
    31021 gcaatgagag ggtcttttct tgcttcgtct gactttgccc tgaactgcct tcgcgcaggg
    31081 acaatatttg tactctgcac agacaagatt gagtaagagt gacttcagga ataatggtat
    31141 acacatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc gcaaggagta
    31201 ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt ttctgtaata
    31261 caaatgatgt attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtcacg
    31321 ttgacggagg agttagatgc agtctggtca actgcagcac cgttgccacc accgaactgt
    31381 ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct gttaccgaag
    31441 ccacgttggg tcagatcgat tgagctgtca tctgagccct gacctacatc tgcaccatta
    31501 ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg cagagcaagt
    31561 gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc gctattatta
    31621 ccgccaccac cgtggttacc gccgccgccg tactgaggaa caacacctgc cagagcgcta
    31681 ccggagaata cgattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat
    31741 cggattgatt taaaagtcga atggaaatta acgttgtgtc acgcgaatag ccatttgcga
    31801 ctgtctctgc actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt
    31861 accagaacct ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc
    31921 atcgttggca ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctggtcaat
    31981 ctttgcccgg ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg
    32041 ccgtaactga gcactattat tcgtcccagc ttgaccaatt atggctgcct gattaaatga
    32101 agacttactc aattcattta ccgcgaagtt atattctgaa ttagctaaat cataacctgc
    32161 tgcggctgca atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt
    32221 catgttgtca ccctggacct ggtcgtacat ttaagaaatt aaatcatttc aacttggttg
    32281 ttaacgcaac ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata
    32341 tttttaataa ctcacccgcg cgttgtattt ttcttttttt gttcatacca aagtattaaa
    32401 acccacgtaa atacgctgtt atctacgcaa aaaatatttt gttttctttt aaatctccgt
    32461 tttccgctat caaaaatcac cagacagtca tttttcttgc ccgtcgctga ttgctgcgat
    32521 atgtcttgcg cacaagccgt gacaaacctc gtctttttgc agtaaaaaat tgtccacgga
    32581 ggtgcggaga aaaaacaaga acgttttaca tgacgaaagg actacaccga aatatttttt
    32641 atatgtatta ttagtaagtt atcaccattt gtatgatttt ttaaaattgt gcaataaaaa
    32701 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaagat taacatttta
    32761 atataacgag ttacatttag ttacatgttt aacacttgat ttaaaatttg taatggctag
    32821 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
    32881 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
    32941 caggtgtgcg atcaataaaa aaagcggggt ttcatcatgt ttaatgaagt ccatagtatt
    33001 catggtcata cattattgtt gatcactaaa ccgtctttgc aagcgacagc tctcttgcag
    33061 caccttaaac aatcgctggc aattacagga aaattacata atattcaacg ttctctggac
    33121 gatatatctt caggctctat tattcttctg gatatgatgg aagcagataa aaagcttatc
    33181 cattattggc aggatacttt gagcaggaaa aacaacaata tcaaaatatt gttgctaaat
    33241 acgcctgaag attacccgta ccgcgacatt gaaaactggc ctcatatcaa cggcgttttt
    33301 tatgccatgg aggatcaaga acgtgttgtc aatgggttgc aaggcgtcct gcgcggcgaa
    33361 tgctacttta cgcaaaagct tgccagttac ctgattacac attctggtaa ctatcgttat
    33421 aacagcacgg aatcagccct ccttactcat cgggaaaaag agatcctgaa taagctgcgt
    33481 atcggcgcgt ctaataacga gatcgctcgt tcgttgttca tcagcgaaaa tacggttaaa
    33541 acgcatcttt ataatctttt caagaagata gccgtaaaaa accggacaca agcggtttcc
    33601 tgggcaaacg ataacctcag gcgataaagc catgaaacgt tatttacgct ggattgtggc
    33661 ggcagaattt ctgttcgccg cagggaatct tcacgccgtt gaggtagaag tcccgggatt
    33721 gttaactgac catactgttt catctattgg ccatgatttt taccgagcct ttagtgataa
    33781 atgggaaagt gactatacgg gtaacttaac gattaatgaa aggcccagtg cacgatgggg
    33841 aagctggatc actataacgg tcaatcagga cgttattttc cagacttttt tatttccgtt
    33901 gaaaagagac ttcgagaaaa ctgtcgtctt tgcactgatt caaactgaag aagcactaaa
    33961 tcgtcgccag ataaatcagg cgttattaag tacgggcgat ttggcgcatg atgaattcta
    34021 aataaaaaat tgttcggagg ctgcaatgcg tgtcaaacat gcagtagttc tactcatgct
    34081 tatttcgcca ttaagttggg ctggaaccat gactttccag ttccgtaatc caaactttgg
    34141 tggtaaccca aataatggcg cttttttatt aaatagcgct caggcccaaa actcttataa
    34201 agatccgagc tataacgatg actttggtat tgaaacaccc tcagcgttag ataactttac
    34261 tcaggccatc cagtcacaaa ttttaggtgg gctactgtcg aatattaata ccggtaaacc
    34321 gggccgcatg gtgaccaacg attatattgt tgatattgct aaccgcgatg gtcaattgca
    34381 gttgaacgtg acagatcgta aaaccggaca aacctcgacc atccaggttt cgggtttaca
    34441 aaataactca accgattttt aagccccagc ttcataagga aaataatcat gcagcgctta
    34501 tttcttttgg ttgccgtcat gttactgagc ggatgcttaa ccgccccgcc taaagaagcc
    34561 gccagaccga cattaatgcc tcgagctcag agctacaaag atttgaccca tctgccagcg
    34621 ccgacgggta agatctttgt ttcggtatac aacattcagg acgaaaccgg gcaatttaaa
    34681 ccctacccgg caagtaactt ctccactgct gttccgcaaa gcgccacggc aatgctggtc
    34741 acggcattga aagattctcg ctggtttata ccgctggagc gccagggctt acaaaacctg
    34801 cttaacgagc gcaagattat tcgtgcggca caagaaaacg gcacggttgc cattaataac
    34861 cgaatcccgc tgcaatcttt aacggcggca aatattatgg ttgaaggttc gattatcggt
    34921 tatgaaagca acgttaaatc gggcggtgtt ggggcaagat attttgggat cggtgcagac
    34981 acgcaatacc
//
LOCUS       DAEVZA010000037        33907 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEVZA010000037 DAEVZA010000000
VERSION     DAEVZA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.037"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..571
                     /gene="scpB"
                     /locus_tag="LAF92_001897"
     CDS             <1..571
                     /gene="scpB"
                     /locus_tag="LAF92_001897"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBI9790916.1"
                     /translation="SGGRDPLSYDDPLRQITRMIQKFPKPIISMVEGSVWGGAFEMIM
                     SSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKELIFTASPITAQRALA
                     VGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHTMNSDEFERIQG
                     MRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            595..2073
                     /gene="scpC"
                     /locus_tag="LAF92_001898"
     CDS             595..2073
                     /gene="scpC"
                     /locus_tag="LAF92_001898"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBI9790917.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNNYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2070..2966)
                     /locus_tag="LAF92_001899"
     CDS             complement(2070..2966)
                     /locus_tag="LAF92_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9790918.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(3133..3873)
                     /locus_tag="LAF92_001900"
     CDS             complement(3133..3873)
                     /locus_tag="LAF92_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBI9790919.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(3966..4601)
                     /gene="argO"
                     /locus_tag="LAF92_001901"
     CDS             complement(3966..4601)
                     /gene="argO"
                     /locus_tag="LAF92_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBI9790920.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(4740..5600)
                     /gene="mscS"
                     /locus_tag="LAF92_001902"
     CDS             complement(4740..5600)
                     /gene="mscS"
                     /locus_tag="LAF92_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBI9790921.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(5958..7037)
                     /gene="fbaA"
                     /locus_tag="LAF92_001903"
     CDS             complement(5958..7037)
                     /gene="fbaA"
                     /locus_tag="LAF92_001903"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBI9790922.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(7252..8415)
                     /gene="pgk"
                     /locus_tag="LAF92_001904"
     CDS             complement(7252..8415)
                     /gene="pgk"
                     /locus_tag="LAF92_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBI9790923.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(8465..9484)
                     /gene="epd"
                     /locus_tag="LAF92_001905"
     CDS             complement(8465..9484)
                     /gene="epd"
                     /locus_tag="LAF92_001905"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBI9790924.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9856..10287
                     /locus_tag="LAF92_001906"
     CDS             9856..10287
                     /locus_tag="LAF92_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBI9790925.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            10310..10888
                     /locus_tag="LAF92_001907"
     CDS             10310..10888
                     /locus_tag="LAF92_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBI9790926.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            10889..11596
                     /locus_tag="LAF92_001908"
     CDS             10889..11596
                     /locus_tag="LAF92_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBI9790927.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            11584..12261
                     /locus_tag="LAF92_001909"
     CDS             11584..12261
                     /locus_tag="LAF92_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBI9790928.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLKN
                     GALQPLC"
     gene            12255..12932
                     /locus_tag="LAF92_001910"
     CDS             12255..12932
                     /locus_tag="LAF92_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBI9790929.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(12904..13617)
                     /locus_tag="LAF92_001911"
     CDS             complement(12904..13617)
                     /locus_tag="LAF92_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBI9790930.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(13614..14123)
                     /gene="fumE"
                     /locus_tag="LAF92_001912"
     CDS             complement(13614..14123)
                     /gene="fumE"
                     /locus_tag="LAF92_001912"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBI9790931.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(14145..15110)
                     /gene="yggF"
                     /locus_tag="LAF92_001913"
     CDS             complement(14145..15110)
                     /gene="yggF"
                     /locus_tag="LAF92_001913"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBI9790932.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(15107..16384)
                     /locus_tag="LAF92_001914"
     CDS             complement(15107..16384)
                     /locus_tag="LAF92_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBI9790933.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(16399..17787)
                     /gene="cmtA"
                     /locus_tag="LAF92_001915"
     CDS             complement(16399..17787)
                     /gene="cmtA"
                     /locus_tag="LAF92_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBI9790934.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMFGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(17815..18258)
                     /gene="cmtB"
                     /locus_tag="LAF92_001916"
     CDS             complement(17815..18258)
                     /gene="cmtB"
                     /locus_tag="LAF92_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBI9790935.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(18572..20563)
                     /gene="tkt"
                     /locus_tag="LAF92_001917"
     CDS             complement(18572..20563)
                     /gene="tkt"
                     /locus_tag="LAF92_001917"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBI9790936.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNVKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            20841..21599
                     /gene="loiP"
                     /locus_tag="LAF92_001918"
                     /gene_synonym="yggG"
     CDS             20841..21599
                     /gene="loiP"
                     /locus_tag="LAF92_001918"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBI9790937.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(21685..23247)
                     /locus_tag="LAF92_001919"
     CDS             complement(21685..23247)
                     /locus_tag="LAF92_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI9790938.1"
                     /translation="MERTLPWKPLLCLLFLLSTGCDRAEKQKIAAQLQQDKEAISSVV
                     KKSYRTLCGPEFVTSTDSHDRLAEMQQVFTPALMEPVLKYWQESPKTCKRRFPSYSDP
                     LATFVEGDKAQIGAFFGHQSYIDIPMELIREGDGKWKIAAIDFASLHEFEKNGRVTDE
                     VQKKIDLLKKRSFKQHRDFPLLTRTAPERVSKSPLTSVEQTVTEPDSSWLDVLLTIGK
                     YALYLVAIILILALVAVLWIGYGLVFAGHRLFKASCKDPSIQKIPAAERWAMAVGAPY
                     AIAGNNHWARRVVNDDSVEAENDCKSEVESLADMWGVFDRDSLLEQLLALFVAGHRSV
                     YAEQIKNDSEMPDAEYRAFAQQLAQNAKSSSEAKERLWQLQMVRKNRRKICDIDFCAW
                     DMVRFVMLCNSGAQVGYITQREMVDFSMLAARRVQQHYRSWRELAGHFLLARWYWKAT
                     DKRHLITHILFKKAITSLLKEQGSPWRTLPWDTPLPEGTETAFAWACGVSTADVVEVQ
                     PDTPDKAIYEAN"
     gene            complement(23398..24318)
                     /gene="speB"
                     /locus_tag="LAF92_001920"
     CDS             complement(23398..24318)
                     /gene="speB"
                     /locus_tag="LAF92_001920"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBI9790939.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24454..25185)
                     /locus_tag="LAF92_001921"
     CDS             complement(24454..25185)
                     /locus_tag="LAF92_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790940.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(25331..27307)
                     /gene="speA"
                     /locus_tag="LAF92_001922"
     CDS             complement(25331..27307)
                     /gene="speA"
                     /locus_tag="LAF92_001922"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBI9790941.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(27316..27447)
                     /gene="yqgB"
                     /locus_tag="LAF92_001923"
     CDS             complement(27316..27447)
                     /gene="yqgB"
                     /locus_tag="LAF92_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBI9790942.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            27583..27798
                     /locus_tag="LAF92_001924"
     CDS             27583..27798
                     /locus_tag="LAF92_001924"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790943.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            28102..29256
                     /gene="metK"
                     /locus_tag="LAF92_001925"
     CDS             28102..29256
                     /gene="metK"
                     /locus_tag="LAF92_001925"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBI9790944.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            29692..31086
                     /gene="galP"
                     /locus_tag="LAF92_001926"
     CDS             29692..31086
                     /gene="galP"
                     /locus_tag="LAF92_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBI9790945.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            31163..31660
                     /locus_tag="LAF92_001927"
     CDS             31163..31660
                     /locus_tag="LAF92_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBI9790946.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            31755..32462
                     /gene="endA"
                     /locus_tag="LAF92_001928"
     CDS             31755..32462
                     /gene="endA"
                     /locus_tag="LAF92_001928"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBI9790947.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAAGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            32542..33273
                     /gene="rsmE"
                     /locus_tag="LAF92_001929"
     CDS             32542..33273
                     /gene="rsmE"
                     /locus_tag="LAF92_001929"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBI9790948.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            33286..>33907
                     /gene="gshB"
                     /locus_tag="LAF92_001930"
     CDS             33286..>33907
                     /gene="gshB"
                     /locus_tag="LAF92_001930"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBI9790949.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDG"
BASE COUNT         8358 a         9000 c         8358 g         8191 t
ORIGIN      
        1 gtctggcggt cgcgatccgc tctcctatga tgatccattg cgtcaaatca cccgcatgat
       61 ccaaaaattc ccgaaaccga tcatttcgat ggtggaaggt agtgtttggg gtggcgcatt
      121 tgaaatgatc atgagttccg atctgattat cgccgccagt acctcaacct tctcaatgac
      181 gcctgtaaac ctcggcgtcc cgtataacct ggtcggcatt cacaacctga cccgcgacgc
      241 gggcttccac attgtcaaag aactgatttt taccgcttcg ccaatcaccg cccagcgcgc
      301 gttggctgtc ggcatcctca accatgttgt ggaagtggaa gaactggaag atttcacctt
      361 acaaatggcg caccacatct ctgagaaagc gccgttagcc attgctgtta tcaaagaaga
      421 gctgcgtgta ctgggcgaag cgcacaccat gaactccgat gaatttgaac gtattcaggg
      481 gatgcgccgc gcggtttatg acagcgaaga ttaccaggaa gggatgaacg ctttcctcga
      541 aaaacgtaaa cctaatttcg ttggtcatta atccctgcga acgaaggagt aaaaatggaa
      601 actcagtgga caaggatgac cgccaatgaa gcggcagaaa ttatccagca taacgacatg
      661 gtggcattta gcggctttac cccggcgggt tcgccgaaag ccctacccac cgcgattgcc
      721 cgcagagcta acgaacagca tgaggccaaa aagccgtatc aaattcgcct tctgacgggt
      781 gcgtcaatca gcgccgccgc tgacgatgta ctttctgacg ccgatgctgt ttcctggcgt
      841 gcgccatatc aaacatcgtc cggtttacgt aaaaagatca atcagggcgc ggtgagtttc
      901 gttgacctgc atttgagcga agtggcgcaa atggtcaatt acggtttctt cggcgacatt
      961 gatgttgccg tcattgaagc atcggcactg gcaccggatg gtcgagtctg gttaaccagc
     1021 gggatcggta atgcgccgac ctggctgctg cgagcgaaga aagtgatcat tgaactcaat
     1081 aactatcacg atccgcgcgt tgcagaactg gcggatattg tgatccctgg cgcgccaccg
     1141 cggcgcaata gcgtgtcgat cttccatgca atggatcgcg tcggtacccg ctatgtgcaa
     1201 atcgatccga aaaagattgt cgccgtcgtg gaaaccaact tgcccgacgc cggtaatatg
     1261 ctggataagc aaaatcccat gtgccagcag attgccgata acgtggtcac gttcttattg
     1321 caggaaatgg cgcatgggcg tattccgccg gaatttctgc cgctgcaaag tggcgtgggc
     1381 aatatcaata atgcggtaat ggcgcgtctg ggggaaaacc cggaaattcc tccgtttatg
     1441 atgtattcgg aagtgctaca ggaatcggtg gtgcatttac tggaaaccgg caaaatcagc
     1501 ggggccagcg cctccagcct gacaatctcg gccgattccc tgcgcaagat ttacgacaat
     1561 atggattact ttgccagccg cattgtgttg cgtccgcagg agatttccaa taacccggaa
     1621 atcatccgtc gtctgggcgt catcgctctg aacgtcggcc tggagtttga tatttacggg
     1681 catgccaact caacgcacgt agccggggtc gatctgatga acggcatcgg cggcagcggt
     1741 gattttgaac gcaacgcgta tctgtcgatc tttatggccc cgtcgattgc taaagaaggc
     1801 aagatctcaa ccgtcgtgcc aatgtgcagc catgttgatc acagcgaaca cagcgtcaaa
     1861 gtgatcatca ccgaacaagg gatcgccgat ctgcgcggtc tttccccgct tcaacgcgcc
     1921 cgcactatca ttgataattg tgcacatcct atgtatcggg attatctgca tcgctatctg
     1981 gaaaatgcgc ctggcggaca tattcaccac gatcttagcc acgtcttcga cttacaccgt
     2041 aatttaattg caaccggctc gatgctgggt taatgactcg tcccgtcacg atcttcagca
     2101 atatgctgaa gcatcgtggc ggtataacga tgatttctga ttgaataaag atattcctgc
     2161 atatacatcg gcctgtgcgg cgcatcaaaa tatttcagcg ttgccggatt caccagtcgc
     2221 caggcaaaat gcgggatcac cgtcagaaaa tgtccgcgct ccacggcgct gattttggcc
     2281 ataaaactgt agggacgata aataatggtt ggattgatcc cacaaggtcg catattcgca
     2341 tcaatcattg cttcgaagtt ggcccggttc tgaaagcgca tttgtagcca gggcagctcc
     2401 tgaagcagct cttgctcttg acgctcttca taacggcgag aaacgagaaa gcctaatcgc
     2461 agcggtggta gttcactgat agtcaggttt tccagttcct gaactcgtgc agatacatgc
     2521 tgtggggaga taataaaatc taactggcgg tcaaaaagat tatcgataac gccgttttcg
     2581 ctgaactcaa ctggctgggc ggtcacgcct tcatacttat ctccgagact aatcaactga
     2641 tcgaaaataa ttgtcggata agtattatct atgccaatca caatttctcg tgaacgacgt
     2701 cctgagttat ggatttcatt atcaatcgca gaaagccgtt gataaaccgg gaacagtttt
     2761 tgatacagtt cctgtccggc tttattgagg ctgatattat tatctttgcg ggtaaatagc
     2821 gtatagccaa tttgttcttc cagcgcggca atgcttttac caaatggcga agccgtcata
     2881 tggatttttt ctgccgcccg ggcaatatta ttggtttgcg ctaataaaat gaaattgcgc
     2941 atctttttcg aaataaaaat gtccatcccc ctcccccgca tccttgtccg cttaaccgtt
     3001 tcagtcagcc tatccttgat gaaaccgcga gcaaagatag gtgattacgt catggtttta
     3061 cagaaaatta cagaaaaagg aggcaatatc gggtaaaggc attagcccga cgaatacgtc
     3121 gggctacaag tattattgtg ctgcaggtgt tttagcgggt tgttgatcca caggttctaa
     3181 ctggaagacc acatcgacct gatcatcaaa ctgaatagcg gcctgctcgt aagtttcctg
     3241 ggcggacacc ggcgcggcat cggctttcat catccgcacc attgggctgg gctgatagtt
     3301 ggaaacatgg tagcgcacgc tatataccgg ccccagttta cgatgaaagc cgttcgccag
     3361 ttcctgcgcc tgatgaatcg cgttatcaat cgctgcctta cgcgctttgt ctttataggc
     3421 atccggctgc gccacgccca gcgacacaga acgaatttcg ttaagacccg ccttcagcgc
     3481 gccatccagc aaggaattca gtttgtctaa ctgacggagc gtgacttcca ccgttctcac
     3541 agcgcggtag cctttcagga tacttttacc atcctgataa tcataatctg gctgggtgcg
     3601 taagttcgct gagctgatat cttttttcgc gatctgattg agttcaagga aggaaatgta
     3661 ttgtgcgacg cgctcatctg cctgtttctt ggcagtagcg gcatccttcg cggccacgtt
     3721 aacttcaatc gcaagagtgg caatgtctgg caccgcatcc acgcttgccg taccggaggt
     3781 gacaatatgc ggtccatccg gcaattcgtt agcctgcgct gccatcccgc taatacccat
     3841 taatgccgcc agggcgataa ctttgaactt cacgttagtc tctccgtgtt aattgacttg
     3901 cccaaaattc aggacctctg gaggcaagct tagcgcctct gttttatttt tccatcagat
     3961 agcgcctaac tgaacaaggc ttgtgcatga gcaataccgt ctctcgccag ctgcaaggct
     4021 ataaaccaca tgacacatcc cactaccaga ttgataatgc gctgtgattt tgccgtgcgc
     4081 aggcgcggtg ccagccaggc tgcgagaata gccagaccaa agaaccacag gaaagaggcg
     4141 ctaattgtcc cgagcgcaaa ccaacgtttt ggttcgacgt caagctgccc gccaaggctg
     4201 cccagtacaa caaaagtatc caggtaaaca tgcggattca gccaggtcac tgccaacatg
     4261 gtggcgataa ttttccatct gccttgcttc aggacttcgg cactggctaa ctcaatatta
     4321 ctgctcattg cggttttaaa agcgccaaaa ccataccaca gcaagaaggc tacgccgccc
     4381 caggtgacca gcgccagcaa ccacggcgac tgcatcaata acgcgctgcc accaaaaatc
     4441 ccggcgcaaa tcaggaccaa atcgctgata gcacaaagta aggcaatcat aatgtggtac
     4501 tgacgacgaa tgccctgatt catcacaaaa gcattttgtg gaccgagcgg taggatcata
     4561 gctgcgccaa gcgcaagacc ttgaaagtaa taagaaaaca cgttggatat tccgaattaa
     4621 gtatctgatg cagagactat atcgcgggat tgttattagc ggaaattgat aatattaatt
     4681 ggcaatcaga aaaactaata agagagaggc cagagagtat tatctggcct cagcgttgat
     4741 tacgcagctt tgtcttcttt cacccgctta aagttcacat ccatttgcgg gtacgggaag
     4801 ctgataccgg cggcatcaaa ttcacgttta atacgctcca gcacatccca gtacacgttt
     4861 tgcagatcgc cgctgttgct ccagacgcgg accacgaaat taatcgacga tgcaccaagt
     4921 tcgttcaggc gcacagtcat ttcgcgatct ttcaaaatgc gatcttcaga ctggataata
     4981 ttggtcagga tctgcttaac ctgatcgata tcggaatcat acgccacgcc aataataaat
     5041 tcgttacggc gaactggctc gcgggagaag ttaataatat ttccggcaat aattttaccg
     5101 ttcggaataa cgataatttt accgtctgca gtacgcatgg tggtggagaa aatctgcaca
     5161 ctcagcacag taccggctac gccgccgagg tcaacatatt ctccggcacg gaacgggcgg
     5221 aacatgacaa gtaacacgcc agcggccagg ttagaaagtg acccctgcaa agccagacca
     5281 acagctaagc ctgcggcacc gagtacagca atgactgacg ctgtttgtac acccacgcgt
     5341 cccagggctg cgattagcgt aaaggcgata ataccgtaac ggactaatgc agaaagaaaa
     5401 tcagcaacag tggcatcgat tttacgggag atcatcaggc gattcaccgc gttggaaatc
     5461 atccgcgcga taatcaaacc aacgatgatg atcgcgagtg ccgccacgat gtttactgca
     5521 taacttagca gcagcgcctg gttagctacc agccagcttc ccgcgccgtt tatgctatcg
     5581 acaacattca aatcttccat tcaatattcc ttttcaactg actcaaaatg gagaaataca
     5641 ctccgccgcc ttatgacggg cagtctgaca agggtaaaca ataaagcata attttgccaa
     5701 atagatcaca gatttctcat ctgaatcacc ttgcttttgg cgaaaaaatc cccgcagagc
     5761 ggggctcaga cagatatcaa tgtgcgcttt gtcatgccgg atgcggcgtg aacgccttat
     5821 ccggcctaca aaaacctgca aattcaatat attgcaggat acgtgtaggc ctgataagcg
     5881 tagcgcatca ggcaattttg cttttggcga aaaaaagacc cgcagagcgg gccttgagat
     5941 aagcagaaag gaatgactta cagaacgtcg atcgcgttca gttcctggaa tgctttttcc
     6001 agacgagcga tcatcgaagt ctgaccggca cgcagccata cgcgcggatc gtagtatttc
     6061 ttgttcggct gatcttcgcc tttcgggtta cccagctgac cctgcagata agcttcgttc
     6121 gctttgtagt agttcagaac gccttcccag gttgcccatt gggtatcggt atcgatgttc
     6181 attttcacta cgccgtagct tacggagtct ttgatttcct gagcagtaga accggaacca
     6241 ccgtggaata cgaagttcag gctgttgtgc ggcaggttgt gtttcttaga aacatattcc
     6301 tgagaatcac gcaggatggt cggagtcaga accacgttac ccggcttgta aacaccgtgt
     6361 acgttaccga aggacgcagc gatggtgaaa cgcgggctga ttttgctcag ctcggtgtat
     6421 gcgtaatcaa cgtcttccgg ctgggtgtac agtgcagaag cgtccatgtg gctgttgtcc
     6481 acgccgtctt cttcaccacc ggtgcaaccc agttcgattt ccagagtcat gccgattttg
     6541 gacatacgct ccaggtattt agagcagatt tcgatgttct cttgcagaga ttcttcagac
     6601 aggtcgatca tgtgagaaga gaacagcggc ttaccggtag ctgcgaagtg tttttcaccc
     6661 gcgtccagca gaccgtcgat ccacggcagc agtttcttcg cgcagtggtc agtgtgcagg
     6721 ataaccggaa caccataatg ttcagccatc tggtgaacgt gatgcgcacc agagatcgcg
     6781 cccaggatag cagcaccctg cggaacgtca gatttcacgc ctttaccagc gataaaggaa
     6841 gcaccaccgt tggagaactg aacgataacc ggcgctttaa ctttagcagc agtttccagt
     6901 acggcgttga tggagtcagt accgacgcag tttactgctg gcagtgcgaa gttgttttct
     6961 tttgctacct ggaaaacttt ctgtacgtca tcaccagtga ttacgccagg ttttacgaaa
     7021 tcaaaaatct tagacatgtc tcttgtcctg tatcgtcggg ccttatactc atcatacttc
     7081 aagttgcatg tgctgcgtct gcgttcgctc accccagtca cttacttatg taagctcctg
     7141 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagtatg aaaaaatgcg
     7201 tgctctaaaa gcgcgctgaa acaagggcag gtttccctgc cctgtgattt tttacttctt
     7261 agcgcgctct tcgagcatcg ctactgcagg cagtacttta ccttccacga attcgaggaa
     7321 tgcgccgccg ccagtggaga tgtaggagat tttgtcagca atgccgaaca ggtcgattgc
     7381 tgccagagtg tcgccgccgc cagcgatgga gaacgcttcg ctgtctgcga tagcgttagc
     7441 cacgatttca gtacctttgc ggaagttcgg gaattcgaac acgccaaccg gaccgttcca
     7501 cagaatggtt ttcgcattct tcaggatttc agccagttcc tgagcggaag catcaccgat
     7561 atccaggatc tgctcgtcag ctttcacatc gttaacagat ttcagggtag ccggtgcagt
     7621 ttcagagaac tcggttgcta cgcgaacatc agacggaacc gggatgttgc aggtggtcag
     7681 cagacgttta gcttcgtcaa ccaggtcagc ttcgtacagg gatttaccca catcgtggcc
     7741 ttgtgccgcg ataaaggtgt tagcgatacc accaccaaca atcagctggt cagcgatttt
     7801 agacaaggag tccagaacgg tcagtttggt agatacttta gaaccaccaa cgatagccac
     7861 catcgggcga gctggttctt tcagtgcttt acccagcgcg tccagttcag ctgccagcag
     7921 cgggcctgcg cacgcaacgt cagcgaattt accgataccg tgagtagaag cctgcgcgcg
     7981 gtgagcagta ccgaatgcgt ccattacgaa cacgtcacac agtgcagcgt attttttgga
     8041 cagggtttcg tcgtctttct tctcgccttt gttgaagcga acgttttcca gaacaaccag
     8101 ttcaccttca gcaacgtcaa cgccgtcgag gtaatcttta accaggcgaa ccgggttaga
     8161 cagtttgtct ttcaggtagt taacaaccgg cagcagagag aattcttcgt tgtactcgcc
     8221 ttcggtagga cgacccaggt gggaagttac catcactttt gcgccttgtt tcagggccag
     8281 ttcgatggtc ggcagagaag cacggatacg cgcgtcgctg gttactttcc cgtcttttac
     8341 tggtacgttc agatccgcac ggataaatac acgtttccca gcaagatcca gatcggtcat
     8401 cttaattaca gacatggtga atcctctcgt tgattctaaa agttttgcag acgctgcttg
     8461 cgtcttacct gaaagcaaca gtagccatag ctaacgtcgt gtcgagcatt cggttagcaa
     8521 agccccattc gttatcgcac cagaccaacg ttttgatcag gtgtgcgcca ctgacccggg
     8581 tttgggtgcc atcgacaatg gcactgtgcg gatcgtggtt aaaatctaca gagaccaacg
     8641 gcaattccgt atagtcaact ataccatgaa atgcaccttg tgctgctttt tgcagcaaca
     8701 ggttgacttc attggctttt acaggtttct tcaccgtcac gcttaaatcg attgccgtca
     8761 catttatggt tggcacacgt accgcaatcg cttcaaagcg atcgttaaat tgcggaaaaa
     8821 atcgtgtgat accggcggcc agtttagtat cgaccggaat gatcgactgg ctggctgccc
     8881 gggtgcgacg caggtcagga tggtatgcat caataacctg ttgatcgtgc atggcggagt
     8941 gaattgtggt cactgtgccg gactcaatac cgtacgcatc atctaacaat ttgatgacgg
     9001 gaattatgca attcgtggta caggaagcgt tagaaacgat gcggtgttcc gcacgaagtt
     9061 gatcctgatt gacgccgtaa acaacggtcg cgtcgagatc gttactgcca ggatgtgaaa
     9121 agagcacttt tttggccccg gcggcaatat gcgcttcgcc atgctcgcgg gagccatata
     9181 cgccggtgca gtcgaggact acatcaacgc caagttcacg ccaggggagc gattgcagtg
     9241 aacgttcatg caatacgcgg atggcgtcat caccaacaaa aagttgatcg cgttcctgtc
     9301 gtacttccca tgcaaaacgg ccatggctgg tgtcatattt caacaaatgc gccatgcccg
     9361 cagcatccgc cagttcgttg attgccacca cggtaatttc cgcccggcgt ccggattcat
     9421 acaaagcacg aaccacatta cgcccgatgc gaccgaagcc atttatcgct acgcgtacgg
     9481 tcatagatct cctgcaaggt tttccctgag caaatttgcc agacagagta atccagcaaa
     9541 tcgtccgggg aaaccttacc tgtcgcaaac tgcgactgat tggttaattg tcgaacattt
     9601 aatcgactga aacgcttcag ctaggataag cgaaacgtgg aataaaagga atgattgtcc
     9661 agtcaaagaa agcaattatc tgacttacgt cacgttttca gccccgcccc tgtggaattt
     9721 attccacttt gcagaatcac gaataagcgt agcaaaatct tttcgttgtg ccgtcacaaa
     9781 ccctgactta tatcagaata tgactgatgc agcgtcgata ttatccgcaa tacgtccgag
     9841 tttcggagta ctccaatgat gacattgccc tacccatatt cttcctccgc acgcttttac
     9901 gcactgcgcc tcctccccgg gcaggaagtt ttttcccagc tacacgcttt tgcacagcag
     9961 catcaacttc atgccgcctg gattgcaggc tgtaccggta gcctgactga cgtcgccttg
    10021 cgctatgccg ggcaagaagg caccacgctc ctgaacggta cattcgaagt gatctcacta
    10081 aacggcacgc tggaacagag tggtgagcat cttcatctct gcgtttccga tccacacggc
    10141 acgatgcttg gcggtcatat gatgcccggc tgtaccgtgc ggaccacgct tgaactggtg
    10201 atcggtagcc ttgaggaact ggcgtttagc cgccaacctt gcgcgctttc tggttacgac
    10261 gagttgcata tttcccccgt taaataacta ctttcaaaga ggttttctta tggcgcgtag
    10321 ccacttttcc agtcaggcac tggtgttgat cgttatatct atcgccatca atatgatcgg
    10381 cgggcaactg gccagcatgg tcaaattacc tatatttctc gactccatcg gcacacttat
    10441 tagcgcggta ctgcttggtc cggtgatcgg aatgctgacc ggcttactga ccaatttact
    10501 ctggggattg ctgaccgacc cgatcgccgc cgcatttgca cccgtcgcga tggtcatcgg
    10561 cctggtcgcc ggttgcctgg cgcgagcggg ttggtttcgc accctgccaa aagtcgttgt
    10621 cagcggcgtg attattacgc ttgcggtaac ggtcgtcgct gtaccgctgc gtactgcgct
    10681 gttcggcggt gtcaccggta gcggagccga tttgttcgtt gcctggatgc actcgatggg
    10741 gcaaaacctg gtggagtcgg tggcgattac ggtgataggc gcgaacctgg tcgataaaat
    10801 tcttacagcg gtgattgtct ggcttttact gcgccagctg ccgattcgca ccacgcgcca
    10861 ttttccggca atggctgccg tgcgctaaat gcatccattt acctcattaa cactgtgggc
    10921 attggcggcc tgcaccacgc ttatcctgcc cgcgcagacc attttgccaa tctatagcgc
    10981 ggcgaccttt ttctgcctta ttgcgctaaa ggccacgcgg cggcgggcaa aatatgtcgt
    11041 ctggctgatg ttctcgctgg gagccgggct gtggctggta cacggtggct ggctaccaga
    11101 atggctaagc ggcacgccac gctcgccaga acggtggtcg catgctatta cgctctggtt
    11161 gcggattctg gcgattgttt caacctcaca gttgtggatg cagtacgtcc ccgtgcaatg
    11221 ttttattcgc gctctgtttg cttctcgcct gccgccaggc gtcgcttatc tgtttgccgg
    11281 gccgttattg gtcgttgagc agttaaaacg gcaattagcg attattcatg aagcgcagcg
    11341 tgcccgcggc gtgccgctgg atgagggctg gtatcagcgt ttacgtgcga tgcccgcgtt
    11401 aattattccg ctaacacaca atgcgcttaa cgatctggct gtccgtggtg cggcgctgga
    11461 tatgcgggca tttcggatcc acaaccgacg aacaacgtta tgggcaccag ccgatagcac
    11521 cctacaacgc gtagcgcgtt acaccatgat cttgctgatg ctgacagaat tcggagcgtg
    11581 gatatggtta cgttagaaca gttccgttat tgcccgacac actcaacgca tcctccgttc
    11641 tgttatgact ttcactatgt caaaccgggg atggtcgcca tctttggtga caacggcagt
    11701 ggcaaaagta cgctggcaca gctgatggcg ggctggtatc cggactttct gccaggagag
    11761 ataacaggca cggggacgct cctcggtacg cccattgggc atctcccgct taatgaacaa
    11821 tccgccacca tccagctcgt ccagcaatcg ccctatttgc agctctccgg ctgtaccttt
    11881 agcgtggaag aagaagttgc ctttgggcca gaaaatctgt gtcttgctga agcagagatt
    11941 atggcgcgga ttgatgccgc cctgactctg accgagtgcc agccgttgcg ccatcgtcac
    12001 ccggcaacac tctccggtgg agagacacag cgcgtggtca tcgcctgcgc catcgcgatg
    12061 cagccaaagc tcttaattct tgatgaagcc tttagccgcc tgactccgca ggccagcgaa
    12121 atgctgttgc agcgcttaca acactgggcg tttgaacgtg gttcgctgat tattcttttt
    12181 gaacgtcatc gcacaccttt cctgaactac tgccagcaag cgtggcaatt gaaaaacgga
    12241 gcgctccagc cactatgtta acgttaaata agatatctta tcgctggcct ggcgcggcaa
    12301 cggattgtct ttgcgacatt tctctgcaac tcaaacaagg tgaatggttg gcgctgaccg
    12361 gcgacaatgg cgcgggaaaa tcgacgctgt tacgagtaat ggcaggtctt cttacgccca
    12421 cagccggtac ggttatgctg caacaacagg cgatgaaaaa cctgaaaaac cgccagcgcg
    12481 cggcaaaggt tggcgtactg tttcaggaag cggaaaacca actttttcac agcaccgtcg
    12541 ctgacgagat cgcatttggt ttgaaactgc aaaaatgccc ggcggatgaa atcactcaac
    12601 gtacccatgc tgccctgcaa tgttgtcagt tagcagacac tgccaacgcg cacccgttgg
    12661 atctgcatag tgcgcagcga cggatggtcg ccgtcgcctg cctggaagca ctctctccgc
    12721 cactattgct gctggacgag ccgagtcggg attttgacga aaattggttg agcgtgtttg
    12781 aaagctggct ggagaagtgc cgccagcgag gcacgagcgt cgtagcaatc agtcatgacg
    12841 ccgcatttac ccggcgtcat ttctctcgtg tggtgcgact ggaagatggt ctaatcagga
    12901 acgttaatcc accagacgat attcacccgt agcggtcatc attaaggtca aatttgcagg
    12961 cagactctct tccagcaccc ggcgaacatt cggcccgtcg gtacggtcat aaaaagcttc
    13021 tgcgtctgcc agcgataccc ctcccgccag cttgcgccct accagtcgtt cccgcaacgc
    13081 agtagctggc gcgttgataa agatcgagaa atcacaaaat tgcgcaagct ggcaccactt
    13141 ctcgtcgtcc agcaacaacc agtttccttc gacgatgacg agtggtgcgg taacgtgcaa
    13201 cgcatcttca acaggatcat gcttttgtcg atcatactgc ggccacgtac aatccccttc
    13261 cacgacctgg cacagatttt ccgccagttt cgcaacgtca aatgtctctg gtgcgccttt
    13321 gaaggggcgc aattgatgcg catccagcca gctattgtaa tggtgaaaac catccatcgg
    13381 gagcgtctgg atagcgggta attcagggtc ttgttgcgcg agatattccc agaaggtagt
    13441 taaggtagat ttacccgtcc ccggcggagc gcaaagaaaa accaccgtac gtcgctgcgg
    13501 acttaccgtc tgtaaggccg ccagcatacg cagcaacggt ttatgcacgc gttctatctc
    13561 ttcatcatgg tactgcgctt gcacgttcag cccattcacc gttaattcaa ttttcacggc
    13621 gttaattcct ttaaggtggt cgttactgcc agcgtcagcg ccgttttgac cattccgcta
    13681 aaacgcgcct ctgaccagac ggcgaaatcg gcgaacttca tcgcttttaa cgcgtcatag
    13741 agcgtcgcat tgcaggttat gctattgacg ttgctgataa aatcccaggt catgtcatcg
    13801 gcaaatccgg gcgtttcatc ctgttcgttc aggtaatgcc agtactggga aaagtgcgtg
    13861 atatcggcat acagccattt atccattttc cccagcgcat aaatcaaacg cagcgcaaca
    13921 tcaatatcgt caagcgggcc gctttgggcg agtaacggct tcactgcata ttcgacgatc
    13981 tcttcatcgt tatcgacaaa cagcgacggc agaaactggc gtatcccctg gagcaaaata
    14041 gaatgcgcag ttttcataaa tgaaaataaa ttgtcctgtg catccagttg ctcaagcaca
    14101 tcatcttctg tcagcgtcgc cattggtctt ccgatagatc accatcaatg cagggagtct
    14161 attatattac acgtttggtc cgcgccgccg atcagtaatg tctgcgtccg caccccattc
    14221 gccgtctgtt ggataccgtt caccaactcg ccgcccgtca cgcccgtagc gctaaagagg
    14281 atatcgttcc ccctcaccag ttcatcgagc gagtacacac ggttaacgtc aacgcccatt
    14341 gccttacaac gcttgcgctc ctgctctgca atctgccgat tttccgtgta atccccttta
    14401 gcctgacaaa agtcaatcag ctcggcctgc atatccccac ctaatgcttt aacggcacag
    14461 gcagaaatca cgccttctgg cgcaccgccg atggtgtaca tcacgtcgta aggattatcc
    14521 tgccagcagg tcagcacgct ggcggcgaca tcgccatccg gcagagcgaa aaccttcacg
    14581 cccagctgtg ttgcttcttc aatcgcggca ctcaggcgcg gtttatccag cgtaaccatg
    14641 cgcagcttgt ccagcggttt acctaacgcc cgcgccacgt tacgcaagtt gtccgccaga
    14701 ggcaaagaca gatcaatcgc cccggcagcc agtcgattaa caaccagctt tttcatatac
    14761 atatcgggcg catgtaacag gctatctcga ggggcgaaag ccatgaccgc cagcgcattg
    14821 ctctgcccca tcgctaccat ccgcgtgcct tcaatcgggt caaccgcgat atcgacttct
    14881 ggcccatcgc ctttgcccac ctcttcgcca atccacagca tcggcgcatg gtcaatttct
    14941 ccttcgccga taaccactcg tccacgaaaa gcgacgtcgt ttaatgcctg gcgcatcgcg
    15001 gtgaccgcca ggccatcaat tttatttttg tcgccacatc cggtttgcgg ccaggcagcc
    15061 aacgctgcct gttccgtaac gcgaaataat ggccacgcca gggacatcat tgcgcggcct
    15121 ccccgatatc aacgccaaat tgtgccagca gatatttctc ggcctgttca ttccagatgc
    15181 cgtgcgtttc ctcaaccagc cgcgccaact ctttaaacag cggatcggtt ttgccttttt
    15241 cggcaaaatc ggcaatggca gtgagcggca tggtcacgcc gttataaatg agttttttac
    15301 cgccagggat atccggcaga ttgagcacgg tatctggcac cgcatccagc ccaccgatat
    15361 gcgtgaccat aaaggacggc tgcaactgcc ccgtggcgct taacgcaatc gcctctttca
    15421 tgtcgtccgt tgaaccgccg gatgtaccga ctacgtgcgt gctgttgtaa tggacgttgt
    15481 agaaattaaa cggcactttg aagtttttat ccgtcggccc ggcaaagaag ttcagacagc
    15541 catcttccgc cagtaattcg tcagccatct caacgacagc aggcaccgcc gcataaacaa
    15601 aaatgtcatc gaacccgaca tctcctgtca gcgcccgcag cgtctggacc ggatcgctca
    15661 tccctttggt attcacatac accagctcaa tgcctttact ggccgccaga tccaccggca
    15721 gcagcttctg cacctgcgcc aggcgtttgt cgtcgatatc gaccaccacc acccgtgacg
    15781 gttgtatgcc accgttaatg gcgtaatcga tagcgccaat gcccatcgga cccgcacacg
    15841 ccagcagtgc aatattgccg ccaggtttga cgcccatgcg atgctcataa acatattgcg
    15901 tggtgtgata attggcatga taagcaccaa taatgcagca cataggctct gccagcgacg
    15961 ccgcagcaaa ataagagccg tgatacggta atacgcagcc caaattaatg gcgatttcgg
    16021 gaataatcat ataggtggcg ttaccgccaa aatattcgta gctgtagcct gctgaatatc
    16081 cgcttggtaa ccccatcgcc ggttgcaata caaaacgctg gcctttttta tatttgccag
    16141 tgagattttt acccacttcg acaataaccc cggcacattc atgcccggta atgaccggat
    16201 gattttctaa atcgtcgggt acgcgtttat gttcactacc gagtaacgcc gctttccagg
    16261 tcgataaaca gacgctgtca gaaattacac tcactaacag ttcattatcg gtaatttctg
    16321 gcagttcaaa ttcgcgcaga cggacatccc gcttgccata aatagcagca actttggttt
    16381 tcatttttac ctctgtaatc agtgtttatg ttcggcggtt aattgattaa ataaagtgtc
    16441 gagttttgga tcgccaatat agttattaat caatatcagc ggtttatccg tcacacgttt
    16501 cacgcgccct tccagactgg cgtgagtgac gacgatatcg gcatccgcag gcacgttttc
    16561 tatcgcgtaa tgttttactt ctattgccag ccccgctttt tccaggcgtt tacggaaggt
    16621 ggtcgcgccc atcgcactgg agcccatccc cgcatcgcaa acaaaggcga tacgcttaac
    16681 cctgcttaac gagaatgcac cctcttgttt catcgcctta accgcattgg ctgactgagc
    16741 aaactcatct tcgctctccg tttccaccgt tttttccatc ttcagtatca gcgaagtaat
    16801 agcaaaggac accagggtac ctaccgtaac accggcaatt gtcgccagga acgagccttt
    16861 cggcgtcagt gccaggtaag caaagataga ccccggactt gggccagcca ccagaccacc
    16921 gtccagtaag ttaaacatcc aggtaccaaa cataccgccc gcaatcatgg caataatagt
    16981 cagcggcttc atcagcacat atgggaaata cagttcgtgg atcccaccga ggaagtgaat
    17041 aatcatcgcc ccgggcgcag aacgtttact catcccttta ccaaacaagg taaacgccag
    17101 cagcagcccc aggcccggac ctgggttaga ggccaccata aagaagatgg atttaccgtt
    17161 aaccgaagcc tgttgcattc ccagcggata atagacgccc tgatcgatcg cattattaag
    17221 gaaaagcact ttcgccggtt cattgatgac tgacagcaga ggcagataac ccgcatgtac
    17281 cagcgcctcg atacactctt tgacgaaagt attggcaatt aacaccgccg ggccgatcac
    17341 ttcaaaaccc agcagacaaa ggagcatccc cgcgatacct aatgagaagt tattgataac
    17401 catctcaaaa ccggcaggta tgcgtttttc cagtgcctta tcgacatatt ttatgaccaa
    17461 cccaccgagc ggccccatga tcattgagcc aaggaacatc gggatctctg cgccaacgat
    17521 cacaccaata gtacctattc cgcccatgac cgccccgcgt ttaccgccga ccagatgacc
    17581 accagtagaa ccaatcatca cgggcaataa ataggtaatc atcgggccga caattttggc
    17641 gaaatgttca ttcggcagcc aaccggtggg aataaataac gcagttatga agccccaggc
    17701 aataaaagca ccaatattgg ggatgaccat tgcagtcaaa aagcccccaa aagcctggac
    17761 ctttgcacga gcagacttgt tttccataat attatcctgt agaggagaga gtaattatcc
    17821 gcggctgata atgtccgcta attgtttttc tgatgatgct gttaagagtt gttcgagtat
    17881 ttcttcttcg cacagtaatt cacttaacgc ctgaatagcg ccaatgtgcg aatcggcatc
    17941 ggcagcagag agtccgatga gtaatttaat tggttcgtca ttccccggaa aataaacacc
    18001 ttgttcaagt aatgtcaaag acatcccggt tttaagcgcc ccacattccg gtcgcgcatg
    18061 aggcattgcc acgcctggtg cgagaatata ataagggcca ttgttaatgg tggaatcttt
    18121 aattgcctga atgtaattct cgctgatata gtttttatcc agcaatgata ccatcgagaa
    18181 atcgatagct tcctgccaat cttttgctga atgtataact gagattgatg attctggaaa
    18241 ataatcacta agccgcataa attatcctta ttttattttt agggtaccgt caggcggtaa
    18301 aaataaaata ctcgtgcggt ttaatgaaag caattgagca gggaaaccga tgttttgaaa
    18361 acagtgaatt attaatttct tatataacat tgagttatag atataacaac aaatgtcaat
    18421 acgcatatcg tgatgcatat tacataattg atatttattg catataaata tttgtgatct
    18481 acaacacgct ttatctattg cttgtccgca aacggacata tcaaggtaat aaaaaaggtc
    18541 gccgaagcga cctttttacc cgaaatgcta attacagcag ttcttttgct ttcgcaacaa
    18601 cgttatcaac agtgaagccg aactcttcaa acagcagctc tgccggagca gattcaccga
    18661 aagtggtcat accgacgata gcaccgttca ggccaacata cttgtaccag tagtcagcaa
    18721 tacccgcttc tacagcaacg cgtgcagtaa ccgctttcgg cagtacggat tcacggtaag
    18781 cagcatcctg cttgtcaaat gcgtcggtag acggcatgga caccacgcgc gctttcacgc
    18841 cttcggcagt cagtttttcg taggcagcaa cagccagttc aacttctgaa ccggtagcga
    18901 tgaaaatcag ttccggctga ccggcgcagt ctttcagcac ataaccaccg cgcgcgatgt
    18961 ttgccagttg ctcttcagtt cgttcctgct gcgccaggtt ctgacgggag aggatcagtg
    19021 cggtcgggcc gtcctgacgc tcaacaccgt atttccacgc gaccgcggat tcaacctggt
    19081 cacacggacg ccatgtagac atgttcgggg ttacgcgcag agaagcgacc tgctcaaccg
    19141 gctggtgagt cgggccgtct tcgcccagac cgatggagtc gtgggtgtaa accatcacct
    19201 gacgctgttt catcagcgca gccatacgta cggcgttacg tgcgtattcc acgaacatca
    19261 ggaaggtgga ggtgtacggc aggaaaccac cgtgcaggga gataccgtta gcaatcgcgg
    19321 tcataccgaa ctcgcgaaca ccgtagtgga tgtagttacc cgcagcatct tcgttgattg
    19381 ctttagaacc agaccacagg gtcaggttag acggtgccag gtcagcagaa ccgccgagga
    19441 attccggcaa cagcggaccg aacgcttcga tagcattctg cgacgcttta cggctggcga
    19501 ttttcgccgg attagcctgc agtttagcga tgaactcttt cgctttagcg tcgaagtcag
    19561 acggcatttc gcctttcata cggcgggtaa attcagcggc ttcctgcgga taagctttcg
    19621 cgtaagcagc gaatttcacg ttccatgcgg attctttcgc ctggcctgct tctttcgcat
    19681 cccactgagc atagatttca gacgggattt cgaacggtgc atatttccag cccagctgtt
    19741 cgcgggtcag ggcaatttca gcgtcgccca gcggcgcacc gtgggagtcg tgagtaccgg
    19801 ctttgttcgg ggaaccgaaa ccgatgatgg ttttgcacat cagcagggac ggtttgtcag
    19861 tcactgcgcg cgcttcttct actgcgcgtt tgatggatgc cgcgtcatga ccgtcgatgt
    19921 cgcgaataac gtgccagccg taagcttcga aacgcattgc ggtgtcgtcg gtgaaccagc
    19981 cttcaacgtg accatcgata gagataccgt tgtcatcgta gaacgcaatc aatttaccca
    20041 gcttcagcgt acccgccaga gagcaaactt cgtgggagat gccttccatc atgcagccgt
    20101 cgcccatgaa ggcgtaggtg tagtggtcga caatgtcgtg gcccggacgg ttaaactgcg
    20161 ccgccagcgt tttttctgca atcgccatac ctactgcgtt ggcaataccc tgccccagcg
    20221 gaccggtggt ggtttccaca ccagcggtat aacccacttc cgggtgaccc ggagttttag
    20281 agtgcagctg acggaagttt ttcagttctt ccatcggcag atcgtaaccg gtgaggtgca
    20341 gcaggctgta gatcagcatg gagccgtggc cgttggacag cacgaagcgg tcacggtcag
    20401 cccaggacgg attctgcggg ttgtgtttca ggaaatcacg ccacaggact tcggcaatgt
    20461 cagccatacc cataggggca cccgggtgac cggatttggc tttctgtact gcgtccatgc
    20521 tcagcgcacg aatagcattg gcaagctctt tacgtgagga cattttgact ccagatcgga
    20581 tgatgaaggg cacgccctta acgacttgac gacagcgcgt tttgggctac gccggaaaat
    20641 ttgccaacaa tttaccgcaa gccgcgcgtc atgtacatgg aacatccttt tgccgcttca
    20701 gaaatctctg gatcatgctc gcatgttgcg caatctactc gcccgtccgc tgcgcttttc
    20761 cttatactga gactgagcgt cgattcacct gcaaacggcg catttttaga ataatcctga
    20821 ccttgtgcgg aagagaaaac atgaaaattc gcgccttatt ggtagcaatg agcgtggcaa
    20881 cggtactgac tggttgccag aatatggact ccaacggact gctctcatca ggagcggaag
    20941 cttttcaggc ttacagtttg agtgatgcgc aggtgaaaac cctgagcgat caggcatgtc
    21001 aggagatgga cagcaaggcg acgattgcgc cagccaatag cgaatacgct aaacgtctga
    21061 caactattgc caatgcgcta ggcaacaata tcaacggtca gccggtgaat tacaaagtgt
    21121 atatggcgaa ggatgtgaac gcctttgcaa tggctaacgg ctgtatccgc gtctatagcg
    21181 ggctgatgga tatgatgacg gataacgaag tcgaagcggt gatcggtcac gagatggggc
    21241 acgtggcgtt aggccatgtg aagaaaggga tgcaggtggc gcttggtaca aatgccgtgc
    21301 gagtagctgc ggcctctgcg ggcgggattg tcggcagttt atctcaatca caacttggtg
    21361 atctgggcga gaaattagtc aattcgcaat tctcccagcg ccaggaagca gaagccgatg
    21421 attattctta cgatcttcta cgccaacgcg gcatcagccc ggcaggtctt gccaccagct
    21481 ttgaaaaact ggcgaaactg gaagaaggcc gccaaagctc aatgtttgac gaccatccgg
    21541 catccgccga acgcgcccag catattcgcg atcgcatgag cgcggatggg ataaagtgat
    21601 ttcatatttc tctgacagga atgtcggaga aattttcatt cagagccggc cagagtcatt
    21661 aaacaagctc aatcatttgg ggcatcaatt cgcttcgtag attgctttat ctggtgtatc
    21721 cggttgcacc tctactacat cggcagtgct tacgccacaa gcccaggcaa aagccgtctc
    21781 tgttccttca ggtaatggag tatcccaggg taaagtacgc cacggactcc cctgctcttt
    21841 taataaagat gtaatggctt tcttaaaaag aatatgggta atcaggtgcc gtttatccgt
    21901 tgctttccag taccaacggg ccagcaaaaa atgcccggcc aattcgcgcc agcttctgta
    21961 atgttgctgt acgcgtctgg ctgccagcat cgaaaaatcg accatttcac gctgggtaat
    22021 gtatcccact tgcgccccgc tattacatag catgacaaaa cgcaccatat cccaggcaca
    22081 aaaatctata tcgcatattt tacgtcgatt ttttctgacc atctgtagct gccacaaacg
    22141 ctctttcgct tcgctacttg atttcgcgtt ctgagctaat tgctgagcga acgcgcgata
    22201 ctcagcatcc ggcatttcgc tatcgttttt gatctgttct gcataaactg aacggtgccc
    22261 tgctacaaac aaagctaaga gttgctcaag taacgaatcc cgatcaaaga cgccccacat
    22321 gtcagcaagt gactcaactt cacttttgca atcattctct gcttccacgg aatcatcatt
    22381 aacgactcgt cgcgcccagt gattgttacc cgcaatggca taaggcgcgc cgactgccat
    22441 tgcccaacgt tcggcagcag gaattttttg aattgaggga tctttacagg atgctttgaa
    22501 aaggcgatgt ccggcgaaca ccaggccata accaatccac aaaacagcca ccaaggcgag
    22561 aataagtatt atcgctacca gataaagggc atatttgcct attgtcagta agacatccag
    22621 ccaggaagag tctggctccg taactgtttg ctccaccgat gtcaaagggg acttactgac
    22681 ccgctctggc gcggtcctcg tcaacagagg gaaatcccta tgttgcttga atgagcgttt
    22741 tttaagcaaa tctatttttt tctgcacctc atcagtaacc cgaccgtttt tttcaaactc
    22801 atgtaacgat gcaaaatcaa tcgccgcaat cttccattta ccatcccctt cacggattaa
    22861 ttccattgga atatcaatat aagattggtg accaaaaaaa gcaccaatct gcgccttgtc
    22921 gccttcgacg aaggtcgcta aaggatcgct ataactgggg aaacgtcgtt tacacgtttt
    22981 gggtgactct tgccagtatt tcagtacagg ctccattaaa gcaggcgtaa atacttgctg
    23041 catttccgca aggcgatcgt gcgaatctgt gctggtgaca aactcagggc cacataatgt
    23101 gcgataactc tttttgacga cagaggaaat agcttctttg tcttgttgca gctgtgcggc
    23161 aattttctgt ttttctgctc tatcgcagcc agttgataaa agaaataaca aacaaagaag
    23221 aggcttccag ggaagagtgc gttccatcta tttattccat taaaaacacg gtaataacaa
    23281 tccattaaaa aaacagcagt cagggtaaag atgacttaat taacaggata ggttttaaat
    23341 gataagtccc ttgtgccaga caagggactg tgcaaaaaat tcgaccaggc aggttactta
    23401 ctcgcccttt ttcgccgcct gaatatacag catttccagc gccagcgttg ccgctgccag
    23461 agcagtgatt tccgactgat cgtatgccgg agccacttcc actacgtcca tcccaacgat
    23521 gttgagatct ttcaggccgc gtaccagttt aatagcgcga tcggaggtca ggccgccaat
    23581 cactggcgta ccggtgcctg gtgcaaaagc aggatccagg cagtcgatat caaaggtcag
    23641 gtaaaccggc atatcaccca caatctgttt cacctgggcg ataacgtcat ccacgctacg
    23701 atcgttcacc tggcaggcgt ccagtacggt aaagccgttg tctttatcaa actcggtacg
    23761 aataccaatc tgcacggaat gattcgggtc gatcagacct tctttcggcg cggtatagaa
    23821 catggtgccg tggtcaaatt cacaaccgtt cgcgtaggta tcggtgtggg cgtcaaagtg
    23881 taccagcgcc attttaccga aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa
    23941 gtggtcacca ccgaaggaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc
    24001 ctgcagcttc tcgctcatct cacgggcatc gccgaaagca tataccagat caccgcagtc
    24061 cacgacgttc agacgctcac gcatgtcgaa attccacggg aagcggttgt gttcccaggc
    24121 cagattcgtc gaaacctgac ggatagctgc cggaccgtgg cgaccacccg cacgaccaga
    24181 agtggccata tcgaacggca cgccagtaat cacccagtca gcatcgctgt catatggctg
    24241 gaagttcatc ggcaggcgta aaaaaccaaa ggcgttggaa accagtgagt tatcgtattg
    24301 atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc
    24361 gcgtcgttag gcccgacgag gaagggttgg atttgcgaaa cacaagtttg tgctaattat
    24421 cgccttaaat gggcgaggca tcaagcgagg ttgctatttc cgacacggtg gcggcgcata
    24481 gccattgtta tctgccggaa aggcttgtcc agagcacggc ggcagcgcgt tttgcgccgg
    24541 gccttgtaat tcaaaggcgg caaagccgat taccccggta ttacgcacat cgccttgcgc
    24601 cgaattggct gcgtaggaat cagcaacgtt ggcgaactga aaagccgctt cagtatgttt
    24661 atctttgcgg aatcctttaa tcgctaacga atctccggca ttcacaatat agccgttgtt
    24721 attcagcgaa ccttgcttgc cgttcagcac atccaggcca tcaaccgtag caacgatctc
    24781 gtagttggtg ttacgactgt aattacgaac atataattga taattcattc cgactctggc
    24841 gtggacaata tagcgtggtt cattattgga tgaatcataa acacgcgtaa taggtagaga
    24901 attaaaatta ccatcgcgaa tagcatattc cagttcgtta atacgaatgc tataaacgcg
    24961 gtcgtagccg gaaggaacat tagtcgaata actaataacg atcatctccg ccggatcgcg
    25021 ccaggctcgc tcagcagata cgccctgaac cgttgagtgc acatccccac cccaggcggt
    25081 gccgacatta cccgatgcac tgctattata ctgcgcatta ctggaacaac cggccagcag
    25141 cacgattaat gccaccaacg cgctacgtac tttccatttt ttcataaccg ttccctctgc
    25201 atttaattaa tgcagcgatt gtagggagat tatttaataa gaaaagcagc taaattattt
    25261 ttaagaatat ataaaaagaa agcggaccga tttacgtaaa taaatcgacc cgccagcaga
    25321 ataatcttaa ttattcatct tcaagatagg tataaccgta caaacctgcc tcgaactctt
    25381 caaggaactg ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcacggaact
    25441 gagttaacag cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc
    25501 cttcgtcaga cagttccact tcgacactac cgtcagggaa cacgaacacg tcaaccgctt
    25561 cggtatcacc gaacaggttg tgcatgttgc cgaggatctc ctgatatgcg ccgaccataa
    25621 agaaaccgag catcggcgga ttctctggat cgtactccgg cataggcatt gtcgtggcaa
    25681 taccgtcacc atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca
    25741 gcacagcgcg gcgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact
    25801 ggtcgatccc ccacgcatcc ggcatcgact ggaacagcga gaagttgacg tacattttgt
    25861 ccgccatacg ttcctgcagc tcgtcgataa tcggacgatg agcacggttt tgcggatcca
    25921 gctgcttttg cacttcatgg cacatgctca aataaagctg ctcagcccat gcgcgttctt
    25981 gcaggctaaa ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctgactgt
    26041 cgtgtaacca ttcacgcaga gaacggcgag ttcccggttc gtgcatctcc tgccaggttt
    26101 cccacatgct ttgcagcgca cgcggcgcat cttctgcagg cgcggtcggc accgtgtatt
    26161 cgttacgttc cacgccgatg atattagaca ccagcacggt gtgatgcgca gtcactgcac
    26221 gacccgattc agtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa
    26281 tcgcccagat gatgttgttg gcgtattcat tgaggccata gtttacggag cagtcagact
    26341 gcgaacgggt gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat
    26401 tgacgcccag cttgtgcagt tccacataga aacgcgccga ttcacgaacg cctgtggcga
    26461 tatcgcgaat attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga
    26521 gacgcccggc ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga
    26581 acttcgattt ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg
    26641 cacgcacgcc cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga
    26701 tttctgacat cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg
    26761 ccaggcggat atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc
    26821 cagcatgcgc cagcaccgcc atcaactctg ctttcgagcc tgcttccagc cccaacggtt
    26881 cgccagaatg aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa
    26941 caaggaagta atcgccgtta tagccgtagg attcccgcgc acgtttgaac gcggcgttaa
    27001 tggaacgcaa acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac
    27061 cctgtgcttc acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg
    27121 ggtccgggca cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc
    27181 aggcaatatt gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct
    27241 gcatggagcg tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt
    27301 cagacatagc gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc
    27361 ctggaactgc gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc
    27421 caactgcgca accggtttct ttttcataac attattaagc acataaccga acgtaagtgt
    27481 gacagttcgg cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc
    27541 tcagtaactg taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca
    27601 ggtatgcaaa gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag
    27661 gttaagcagc agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg
    27721 aacccacaag ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt
    27781 cgtgtgcttt ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc
    27841 aaaagataaa tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac
    27901 agaatgagac acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc
    27961 atccagatgt taatccatcc ataccgatta acactcagac tgccagtgtt tttaacttgc
    28021 agagtcgtgg taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat
    28081 tctctttagg tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa
    28141 gggcatcctg acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa
    28201 caggatccga aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt
    28261 ggcggcgaaa tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt
    28321 cgcgaaattg gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg
    28381 agcgctatcg gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg
    28441 gaacagggcg cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg
    28501 ctgatgccag cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt
    28561 aaaaacggca ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat
    28621 gacgacggca aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ttctgaagag
    28681 atcgaccaga aatcgctgca agaagcggta atggaagaga tcatcaagcc aattctgccc
    28741 gctgaatggc tgacttctgc caccaaattc ttcatcaacc cgaccggtcg tttcgttatc
    28801 ggtggcccaa tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc
    28861 ggcatggcgc gtcacggtgg cggtgcattc tctggtaaag atccatcaaa agtggaccgt
    28921 tccgcagcct acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat
    28981 cgttgtgaaa ttcaggtttc ctacgcaatc ggcgtggctg aaccgacttc catcatggta
    29041 gaaactttcg gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc
    29101 ttcgacctgc gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa
    29161 gaaaccgcag catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa
    29221 gcgcagctgc tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg
    29281 ccagcctcgc gctggccttt ttcttttacc tcaccgcggt ggataggcat tcacgccgca
    29341 tccggcaaaa agctgcccgc acaataacat cattcttcct caacacgttt tatcgcaaat
    29401 tatcatcact actgaaaccg attacaccaa taacaccagc caaatatttg taatattttc
    29461 atattattat tcagttttca cagttgttac atttcttttc agtaaagtct taattgcaga
    29521 taacagcgtt taatctatga tgatataact caattatttt catgcactta aaccataact
    29581 aagataaatg ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc
    29641 gtactcacct atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac
    29701 gctaaaaaac aggggcggtc aaacaaggca atgacgtttt tcgtctgctt ccttgccgct
    29761 ctggcgggat tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt
    29821 gcagatgaat tccagattac ttcgcatacg caagaatggg tcgtaagctc catgatgttc
    29881 ggtgcggcag tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag
    29941 agcctgatga tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca
    30001 aacgttgaag tactgattct ttcccgcgtt ctgctggggc tggcggtggg tgtggcctct
    30061 tataccgcac cactgtacct gtcagaaatt gcgccggaaa aaattcgcgg cagtatgatc
    30121 tcgatgtatc agttgatgat cactatcggg atcctcggtg cttatctttc tgataccgcc
    30181 ttcagctaca ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg
    30241 ctgctgattg gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caagcgccgt
    30301 tttgttgatg ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc
    30361 gaactggatg aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa
    30421 gagaacagca acttccgccg cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa
    30481 ttcaccggga tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat
    30541 accaacacta ccgagcaaat gtgggggacc gtgattgtcg gcctgaccaa cgtacttgcc
    30601 acctttatcg caatcggcct tgttgaccgc tggggacgta aaccaacgct aacgctgggc
    30661 ttcctggtga tggctgctgg catgggcgta ctcggtacaa tgatgcatat cggtattcac
    30721 tctccgtcag cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc
    30781 atgagtgccg gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc
    30841 gattttggca tcacctgctc caccgccacc aactggattg ccaacatgat cgttggcgca
    30901 acgttcctga ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgcggct
    30961 ctgaacgtac tgtttatcct gctgacattg tggctagtac cggaaaccaa acacgtttcg
    31021 ctggaacata ttgaacgtaa tctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac
    31081 gattaatctc ctcaggcttc ctcccgtcgc ggaggaagcc acctcttgca gtcatctttt
    31141 cttcgctcta tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccaacaggc
    31201 cgttatgcgc cgcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta
    31261 cccggagcca aaactctctt acacccagcg cggaacctcc gccggaacag cctggctgga
    31321 aagctatgaa atccgcctca atcccgtttt gctgttggaa aacagtgaag cctttattga
    31381 agaagtggtg ccgcacgaac tggcacattt gctggtatgg aaacatttcg gccgcgtagc
    31441 gccacatggc aaagagtgga agtggatgat ggaaagcgtg ctgggcgttc ccgcccgtcg
    31501 tacgcatcag tttgaactgc aatccgtgcg ccgcaacacc ttcccctacc gctgcaagtg
    31561 ccaggagcat cagcttaccg tacgccgtca taatcgcgta gttcgtggcg aagccgtcta
    31621 ccgctgtgtt cactgcggtg aacagctggt cgcaaaataa ccacctgaac tatcaggaac
    31681 tttcctgatc tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta
    31741 acacggagta agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcgcagcat
    31801 tttccggccc ggcgttggcc gcaggtatca atagtttttc tcaggcgaaa gccgcggcgg
    31861 taaaagtcca cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg
    31921 gcaaaaaagg cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc
    31981 gagccagccg cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc
    32041 agtgctggca ggacggtgga cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg
    32101 aaagcgatat gcataacctg cagccgtcag tcggtgaggt gaatggcgat cgcggcaact
    32161 ttatgtacag ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg
    32221 atttcaaaga aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct
    32281 acttctatat gcgcgaccaa tacaacctga cactctctcg ccagcaaacg cagctgttca
    32341 acgcatggaa caagatgtat ccggttaccg actgggagtg cgagcgcgat gaacgcatcg
    32401 cgaaggtgca aggcaatcat aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct
    32461 aacctacact agcgggactc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg
    32521 agacgacgcg gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc
    32581 agccattctc acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc
    32641 atggggccgg ggcaggcgtt gcaattgttt gacggtagca accaggtctt tgacgccgaa
    32701 attaccagcg ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat
    32761 cgcgaatctc cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa
    32821 tttacgatcc agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag
    32881 cgctgcggcg ttaaactgga tagtgaacgt cttaacaaga agctccagca gtggcagaag
    32941 attgcaattg ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat ccgcccagcg
    33001 atggatctgg aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg
    33061 cgcgccagta acagcatcaa tacgttgccg ttaccggttg aacgcgtccg cctgctgatt
    33121 ggcccggaag gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact
    33181 gatatcctgt tgggacctcg cgttttgcgt acagagacaa ctgcgctcac cgccattacc
    33241 gcgctacaag tacgatttgg cgatttgggc taacggagaa gaataatgat caagctcggc
    33301 atcgtgatgg accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg
    33361 ctggaagcac agcgtcgtgg ttacgaactt cactatatgg agatgggcga tctgtatctg
    33421 atcaatggcg aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa ctacgaagag
    33481 tggttttcat tcgtcggtga acaggatctg ccgctggccg atctcgatgt gatcctgatg
    33541 cgtaaagacc cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc
    33601 gaagagaaag ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa
    33661 ctgtttaccg cctggttctc tgacttaacg ccagaaacgc tggttacgcg caataaagcg
    33721 cagctaaaag cgttctggga gaaacacagc gacatcattc ttaagccgct ggacggtatg
    33781 ggcggcgcgt cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa
    33841 accctgactg agcatggcac tcgctactgc atggcgcaaa attacctgcc agccattaaa
    33901 gatggcg
//
LOCUS       DAEVZA010000038        33870 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEVZA010000038 DAEVZA010000000
VERSION     DAEVZA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.038"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1409)
                     /gene="tssI"
                     /locus_tag="LAF92_001931"
     CDS             complement(<1..1409)
                     /gene="tssI"
                     /locus_tag="LAF92_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI9790950.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIR"
     gene            complement(1619..2137)
                     /locus_tag="LAF92_001932"
     CDS             complement(1619..2137)
                     /locus_tag="LAF92_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBI9790951.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            2834..3334
                     /gene="tssB"
                     /locus_tag="LAF92_001933"
     CDS             2834..3334
                     /gene="tssB"
                     /locus_tag="LAF92_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBI9790952.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            3369..3593
                     /locus_tag="LAF92_001934"
     CDS             3369..3593
                     /locus_tag="LAF92_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790953.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            3644..5118
                     /gene="tssC"
                     /locus_tag="LAF92_001935"
                     /pseudo
     CDS             3644..5118
                     /gene="tssC"
                     /locus_tag="LAF92_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            5125..5538
                     /gene="tssE"
                     /locus_tag="LAF92_001936"
     CDS             5125..5538
                     /gene="tssE"
                     /locus_tag="LAF92_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBI9790954.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            5542..7392
                     /gene="tssF"
                     /locus_tag="LAF92_001937"
     CDS             5542..7392
                     /gene="tssF"
                     /locus_tag="LAF92_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBI9790955.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            7356..8438
                     /gene="tssG"
                     /locus_tag="LAF92_001938"
     CDS             7356..8438
                     /gene="tssG"
                     /locus_tag="LAF92_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBI9790956.1"
                     /translation="MDGKNRAASSYLSPGNPSADKEQNEPLAQVFHNACSYNFFAMAE
                     LLHRLAQGEKGTPELSLRDDPTQETLRFSADASLAFPLSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            8463..9743
                     /gene="tagH"
                     /locus_tag="LAF92_001939"
     CDS             8463..9743
                     /gene="tagH"
                     /locus_tag="LAF92_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HBI9790957.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            9740..10264
                     /gene="tssJ"
                     /locus_tag="LAF92_001940"
     CDS             9740..10264
                     /gene="tssJ"
                     /locus_tag="LAF92_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBI9790958.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            10267..11598
                     /gene="tssK"
                     /locus_tag="LAF92_001941"
     CDS             10267..11598
                     /gene="tssK"
                     /locus_tag="LAF92_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HBI9790959.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            11603..12364
                     /gene="icmH"
                     /locus_tag="LAF92_001942"
     CDS             11603..12364
                     /gene="icmH"
                     /locus_tag="LAF92_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HBI9790960.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            12373..15132
                     /gene="tssH"
                     /locus_tag="LAF92_001943"
     CDS             12373..15132
                     /gene="tssH"
                     /locus_tag="LAF92_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HBI9790961.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLTMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADCKGAGALTDVADSLLARYAKNMTEDARNDRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTNTSRMTEVLVQYDETLTALDELEAAWHQQQTLVQEIIALRQQLLGMAEDD
                     AASLPHVDAVEDTPPESEQDNTGAKLADEADREQPEETAETVSPVQRLAQLTAELDAL
                     HNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLAIA
                     SLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSE
                     FQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAF
                     DKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALL
                     ARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVTRAEN
                     GARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDALDDESVTE
                     DEPVL"
     gene            15129..15872
                     /gene="tagO"
                     /locus_tag="LAF92_001944"
     CDS             15129..15872
                     /gene="tagO"
                     /locus_tag="LAF92_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HBI9790962.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            15877..17289
                     /gene="tssA"
                     /locus_tag="LAF92_001945"
     CDS             15877..17289
                     /gene="tssA"
                     /locus_tag="LAF92_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HBI9790963.1"
                     /translation="MAMDLRDPNVWVSHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            17398..20832
                     /gene="tssM"
                     /locus_tag="LAF92_001946"
     CDS             17398..20832
                     /gene="tssM"
                     /locus_tag="LAF92_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HBI9790964.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDLPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNSVIIREDIIAQLKTAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLVGANGGAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            20843..22195
                     /locus_tag="LAF92_001947"
     CDS             20843..22195
                     /locus_tag="LAF92_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HBI9790965.1"
                     /translation="MNSNGLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYVPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            22219..22701
                     /locus_tag="LAF92_001948"
     CDS             22219..22701
                     /locus_tag="LAF92_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBI9790966.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            22745..23659
                     /locus_tag="LAF92_001949"
     CDS             22745..23659
                     /locus_tag="LAF92_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790967.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            23669..24148
                     /locus_tag="LAF92_001950"
     CDS             23669..24148
                     /locus_tag="LAF92_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790968.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNVHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(24285..25070)
                     /locus_tag="LAF92_001951"
     CDS             complement(24285..25070)
                     /locus_tag="LAF92_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790969.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTNKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDMVNRYQ"
     gene            complement(25397..25473)
                     /locus_tag="LAF92_001952"
     tRNA            complement(25397..25473)
                     /locus_tag="LAF92_001952"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25437..25439),aa:Asp,seq:gtc)
     gene            complement(25606..26337)
                     /gene="dnaQ"
                     /locus_tag="LAF92_001953"
     CDS             complement(25606..26337)
                     /gene="dnaQ"
                     /locus_tag="LAF92_001953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBI9790970.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            26402..26869
                     /gene="rnhA"
                     /locus_tag="LAF92_001954"
     CDS             26402..26869
                     /gene="rnhA"
                     /locus_tag="LAF92_001954"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBI9790971.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(26866..27588)
                     /locus_tag="LAF92_001955"
     CDS             complement(26866..27588)
                     /locus_tag="LAF92_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI9790972.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            27622..28377
                     /gene="gloB"
                     /locus_tag="LAF92_001956"
     CDS             27622..28377
                     /gene="gloB"
                     /locus_tag="LAF92_001956"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBI9790973.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            28449..29807
                     /gene="mltD"
                     /locus_tag="LAF92_001957"
     CDS             28449..29807
                     /gene="mltD"
                     /locus_tag="LAF92_001957"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBI9790974.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(29855..30625)
                     /locus_tag="LAF92_001958"
     CDS             complement(29855..30625)
                     /locus_tag="LAF92_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBI9790975.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSSVVAYDLSAHMLGVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALVD
                     AIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(30703..31503)
                     /locus_tag="LAF92_001959"
     CDS             complement(30703..31503)
                     /locus_tag="LAF92_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBI9790976.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            31744..32658
                     /locus_tag="LAF92_001960"
     CDS             31744..32658
                     /locus_tag="LAF92_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9790977.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(32655..33458)
                     /gene="dkgB"
                     /locus_tag="LAF92_001961"
     CDS             complement(32655..33458)
                     /gene="dkgB"
                     /locus_tag="LAF92_001961"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBI9790978.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRTIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(33621..33697)
                     /locus_tag="LAF92_001962"
     tRNA            complement(33621..33697)
                     /locus_tag="LAF92_001962"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33661..33663),aa:Asp,seq:gtc)
BASE COUNT         8379 a         8757 c         8779 g         7955 t
ORIGIN      
        1 cggatggtca tctgcgtctt tgttcccggc aggctgccgg gtgtgcggtt ttcctggtgg
       61 taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc cacaatcacc
      121 tcctgaccca cacgcggtat cgccaggtta ccgaatccgg tgcctgccca cgcctgtgcc
      181 acacggatcc agcatgaact gtcctggttt gacgggttat aacggtccca gttaaatttc
      241 acccgcacgc ggccatgttc atcacagaag atttcctcgc ctgccggtcc cgtcacgacg
      301 gcgctctgcg ggccgtccac cagcggtttc agcaacggct gtggtcgcca tgttctgtct
      361 gccggtatta ccgcaaaatg gttatccagc gtggtgcctg aacccctgcg tcccggtacc
      421 gcctgcggct gttcgccgtg aagatcgctc gccaccacct gccattcccg gttcaggttc
      481 gcctgcggat gccccgtcag cacaattcgc cgtcccggcc atatctccgg cgaacggctt
      541 gttccgcgcg ccacttctgc gttgtttcgc cagccgtcca tctgccagcg ggcaaagttc
      601 tgcccgtggg cgcccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg
      661 tagtcctggt gctggccctc ctggtcaaaa cgccctgccc agccgggacg tttaaaggtg
      721 tagtcttttg tcaccacgga agaaggacgg atttgtgcgc tgtagcggaa ctggctgatg
      781 cagagggtgc tcacctcggt acgggtgttc ggattccagg ggatctcaaa ggactccggc
      841 agataacgca cggtgtcgca caggaccagg ctctggtcgg tacttttttg cgcgtgctcc
      901 tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta atcggtttcg
      961 ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg actccactcg
     1021 gtcacaccgt tttctttcag gatcgtcgcg aggatgcttt cgatgtcctc attctggaag
     1081 atacggaagt tctggcgcag ccctgtgcgc cacagcggtg ggcacacctt catgctgtac
     1141 agcatctggt ttttgtcgtt ctcccccagt tcaaaccagg tcaccacacc tttcacccgg
     1201 cgctgtacgt catcgccctg ccatatcgtc aggtaggcca ttttgtccag tatttgctga
     1261 aattcaaggg agagaaactg ctggctgacc agagagagat cgagggaaaa aagcgaagag
     1321 agtgactggt tcagatgaaa ggagaccacc gcaaaagcat ccggtggcag gccgtccact
     1381 tccagcgtga aacgtaatcc ggttgacata gcaacctccg gggttaagga aaaacagaca
     1441 gttcgctgtt aaagcggctg tgacagtaca gtgttacgct tgcgttcagg cacgctaccg
     1501 ccgaagtttc cggcatccgc catctcccgt gaacgtaacc ccttactgac aacctttctt
     1561 tttacggctt aatacaaaaa cagcatccgg cctgatagcc ggatgctgtt ggcttaactt
     1621 atgcttccag cggtgcgcgc cagtcgtcgg caccggaggt gccagacttg acgtgttccc
     1681 actctatttt gcggtaggcc agtgaaactt cgagcaactg ggtgaactcg cgtttcgccg
     1741 gatcctggca atgcggcata tgcagtttca tatcgacaat cgtcgaatcg gtcaggcggg
     1801 tggtgaaata atgctcctgt ttgccttcca cagaggtacg ccaccagtga agttcagtgg
     1861 ttggcagcat ttcgccggag gccagtgcgt tgtacatcag cggaacagct ttgttcagcg
     1921 ccacggtaaa aatgaacggc ttgtgagcac gctgacccgc aggctggccg gactgaggat
     1981 cggtcgggac agtaacgtta tgcaggaatt cctgcaccag catctcgtct tcgtgcccct
     2041 gcacatagat gttgccgaca gaatcagcgg taaatgcacc tgcagtaata tttccctggg
     2101 tctgaccagt gatggagata taacacggtg ttggcatttg taaactcctt gttaacgtgg
     2161 ttaattacta cttacaatga cctgccgtta atcccagaca ggcagatgca gctcacttca
     2221 cggaaccgca tcatttttca gtatttttta catacaaacc gagctagaat ttacggttgt
     2281 cgtaaaaact atttttaaaa tcattaaatc tcttattcag caataactta ataatatata
     2341 tctggataac catacccaac tgttcaaagt ccgtattgcc tccataaaat ggccaaagct
     2401 gaatcatgaa taaatattgt atttatcctt tatgaattat cattttgata atttttgaga
     2461 tattccttaa aaatacccac ttaatcaatc acaaaacaaa tatataaaca agcaatcatc
     2521 agattagctt atgtttaatg aaattattgt ataattcaat aagttacata aaaacactat
     2581 atttaaatta ttgccataat acacctgacg agaattgcac aggttcacac aatctattta
     2641 acgcttgcag aatacccctc tttacgatcg ataaaaagtc cttttcttat ttattttgaa
     2701 gcgtagaatg cggggtgatg aaggagtggt gattttattt tcaccgccat tagggcggca
     2761 ttgccgatat ttttttgcgg tacaaatatg ccgtgttcat ggcctgtacg agataacatg
     2821 acggatcact tttatgagca aaaaatttga aggcagcgtc gcgcctcgcg agcgtattaa
     2881 tatatcgtat gtcccaaaaa cagatggcca gacggctgaa gtggaacttc cgcttaacat
     2941 gctcgttgtc ggcgataccg gaaatacgca ggaaacatcc ccactggatg aacgtcaggc
     3001 agtttccgtt aacaagcaca atttcggtgc agtgatggct gaagcagcca ttggcctcaa
     3061 ttttaccgtc cccgccacgc tgaagggaag cacaacggac gatgagctga atgtggcgct
     3121 gaatatcaag tcactggatg atttttcacc agatagcgtg gcccggcagg ttccggaagt
     3181 gaataaattg ctggaactgc gtgaagcact gactgcactg aaggggccga tgggtaacct
     3241 tccggcattc cgtacccagc tccaggcatt gctggaaaat gaagagtccc gcgagcagtt
     3301 gctcaaagaa atcggccagg tgagcaataa ataaccacca ctatttcatt aaacagggtt
     3361 atccaggcat gacggcacac aaaaatatct ccgggacata ccacctctct gttgcagaca
     3421 ttctgcaggt ggtttaccag gtatgtttca gcccgtccgt tgaaatcaac caggatggtg
     3481 tggctgcgct gattacaaca ctggatcgcc gtatcagtga tctattggat gagattatcc
     3541 atttttgtga gtttcagcag agcgcgtcgc actggcagcg cgtacttcac tgacggcgaa
     3601 aagccggttt cagaaacaca gctaaaacag aaggattttc agtatgtctt tacaggaaga
     3661 ggaactcgtt tccagtcatg ccgggcagcc tgagcaggca tcgtcgctgc ttgatcaaat
     3721 catggcacag acccgtattc agcccggttc ggaaggttat gacgttgccc gccagggtgt
     3781 gacagccttt attgccagca tcctgcaatc cactgcatct gccgaaccgg tcaataaact
     3841 ggccgtggac agcatgattg cagatattga tgaacgcatc agccgccaga tggatgtcat
     3901 catccatgcg cctgcctttc agcaggtcga gtcgttctgg cgctcactga aaaccatggt
     3961 ggatcgcgtt gatttccgtg aaaacatcaa ggtcaacgtg cttcatgtca ccaagcagga
     4021 acttctggaa gactttgagt ttgcgccaga aattattcag tccggatttt ataagcacgt
     4081 ttattcgtcc ggtttcggac agtttggcgg tgaacccatc gctgccgtgc tgggcgctta
     4141 tgagttcaaa aataccgcgc ctgacatgaa actgctgcaa tacgtcagtg ccgtgggcgc
     4201 aatggcgcac gcaccgttcc tgtcctccgt ttccccggag tttatggggc tgaactcgtg
     4261 gaccgaactg cctaatatca aagatcttta tgccatcttg aaggtccggc ctacaccaaa
     4321 tggcgcgccc tgcgtgactc ggaagattcg cgctatttag ggcttacggc accccgcttc
     4381 ctgcttcgcc agccctattc accaacagat aaccctgtta agaactttaa ctattacgaa
     4441 gatgtcagcc agaaccacga agattatctg tggggtaata cggcctggat gctggcatgc
     4501 aatatcgcag acagttttgc caaataccgc tggtgtccaa acattatcgg tccgcaaagc
     4561 ggcggcgcag tgaaagatct gccggtgcat ctgttcgaaa cgatggggca gattcaggcc
     4621 aaaatcccaa ccgaggtgct ggtcaccgat cgccgcgaat ttgaactggc tgaagagggt
     4681 tttatcaccc ttaccatgcg taaagactct gataatgcag cctttttctc tgcaaactcg
     4741 gtacaaaaac cgaagcactt tccgggaaaa gatgcagaaa ccaactataa gctgggtacg
     4801 cagcttcctt atctcttcat catcaatcgg ttagcgcact acatcaaggt gttgcagcgt
     4861 gaacaactgg ggtcatggaa agaaaggagc gacttagaaa gagagcttaa tacctggatc
     4921 cgtcagtatg ttgccgacca ggaaaacccg cctgcagacg tgcgcagccg caaacctctg
     4981 cgcgctgcca aagttgaggt tatggatgta gaaggcgaac ccggctggta ccaggttgcg
     5041 ctaagcgtga gacctcattt taaatttatg ggggcaaatt ttgagctttc cctggttggc
     5101 cggttagaca gggagtaaca caccatgata ccggatcggg aaagaaccac aggttctctt
     5161 tttgagcgta tggaagcctc ttcggccaga aaccggcagg gaggcagtat tcattcgctc
     5221 aggcaatcca ttcgccagaa cctacgaaac attcttaata cccggtccgg tagttgccgg
     5281 ggtgcgcctg agctgggcat tgatgaaccg gaaggggctg aaaacttccg cgaatccatg
     5341 agtcgggcca tcgagcaatg tatagaacgc tatgaacccc ggatctcaca cgctgaagtg
     5401 caggcagtgg tttcatccgc ctccagcccg ctagatatga cgtttcatat tacagcctgg
     5461 gtgacattta acgaaacgca tgaggtgctt gaatttgata tggcaccgaa tggcagccag
     5521 cattaccggg tggactgaca aatggaattt gaagaacgct atttccggga agaactcgat
     5581 tacctgcgcc agcttagcaa gctgctggca acggaaaaac cccatctggc ccgcttcctg
     5641 gccgaaaaag atgcggatcc ggatattgaa cgcctgctgg aaggggtggc ttttcttacc
     5701 ggcaatctcc gccagaaaat tgaggatgaa ttcccagaac tgacgcacgg gcttattaag
     5761 atgctatggc ctaattacct gcgtccggtt ccggcaatga cccttattga atatacgccg
     5821 gatatggata agtcttctgt accggtgtta atcccccgta atgagcagtt tacaaccaac
     5881 gccggggaaa tcagagttga tgaagtgctg ccctctgatg ctaaaaagga ggagccgcct
     5941 ccctgtacct tcaccctctg ccgggatatc tggctgctgc ccgttcgcct ggagcagatt
     6001 gaaaaccgca gtacgacccg taatggtgtt atcaacatca ccttttcggt cgcaccggga
     6061 acggacttcc gcacgctgga tctgaacaaa cttcgcttct ggctcggcaa tgacgacaac
     6121 tatacccgtg accagcttta tttatggttc tgcgaatact tgcagggtgc cgacctgact
     6181 gtgggtgaac agcatattcg cctgcctgag tttatgctaa aagctgtcgg ttttgagccg
     6241 caggatgcca tgctgccctg gccgaaaaac gtccacagcg gctaccggat ccttcaggag
     6301 tatttctgtt accccgatgc gtttctcttt tttgatcttt gtggttgtcc ggctttgcct
     6361 gacggattgc aggcggaatt ctttaccctg caactgcgtt tttcgcgccc tttgcccgtg
     6421 gacatccggc tgcgccgcga ttccctgcgc ctgtattgcg cacctgccat taatttattt
     6481 atccaccatg cagaagccat cacgctggac aaccggcggg cagactatcc gctggttccc
     6541 agccgccatt acccacaaca ttacgatgta ttttccgtta acagtgtggt gagccaggtc
     6601 caggatatgt tcaggaaaaa agatctgggg cgtcctgttt cgacgcaggc cgcgcgccag
     6661 tggccagcct ttgaaagttt cagccatcag atggaataca gccggaagcg ggaagtggtg
     6721 tactggcatc accggaccaa aacatccctg ttccatcgcg gctttgatca tacccttgcc
     6781 tttatacatg ctgatggcag ttatccgtca gacgaatctc tgctcagtaa tgaagtggtt
     6841 tcggtatcgc tgacctgtac caaccgtgag cttccgtcac aaattcgttc cggcgatatc
     6901 accggcacaa ccggtaaaaa tgcggctgtt gcttcatttc gcaacattac ccgcccgacg
     6961 caaccactct ggccggtcat tgatggcagc ctgcactggt ccctactctc cgccatgaac
     7021 ctgaattatc tgtcattact ggatacggac gcgctgaagc aggtcatcgc caactttgat
     7081 cgccacgcaa tccatcatcc gcagacggca cggctgtcac aacaaaagct ggatgccatt
     7141 gagcgtctgg agacccgccc cgttgatcgc ctgtttacgg gtattcccgt ccggggactg
     7201 gcctccacgc tgtatctgca cccggagccg tttgtctgtg aaggggaaat gtatctgctc
     7261 ggtacggtgc tttcgcattt tctgtcgctg tacgccagcg ttaactcatt ccacatgctg
     7321 accgttgtga acacagaaag ccaggagaca tggaaatgga cggaaagaac cgggcagcat
     7381 cctcttatct gagtccggga aatccctcgg cagataaaga gcagaatgag ccgctggcac
     7441 aggtttttca taacgcctgc tcttacaatt tttttgcgat ggcggagctg ttgcaccgcc
     7501 tggcacaggg tgaaaagggg acgccagaat tatccctgcg cgacgatccg acacaggaaa
     7561 ccctgcgttt cagcgccgat gccagccttg cgtttcccct gagtgatatc agtgcgctga
     7621 aacgggatac gtcaggggca ttcaggatga ccaccacctt tatggggtta caggggagcc
     7681 agtcgcctct ccccggttat tacctcgatc acctggcctg gaaagcggta catgaacaaa
     7741 gtccggtagg tgattttctg gatatgttca gccaccgcct gacccagttt gtctggcata
     7801 tctggcgtaa gtaccgctat cacatcagtt ttcgtaacgg cggtgtggat gcgttctcgc
     7861 agcgcatgta ctcccttgtc gggctgggcc accgccagct tcgcgataag ctggcgatta
     7921 atcacagcaa aatgctggcg tattccggga tcctggcaaa cccggggcga tccccggaaa
     7981 ttatctgtgg cctggtgtcc cactgctttg atttgtcaga agtgacgctg caaaactggc
     8041 agcgtcgcaa ggtggatatt gaaccggatc agcaaaatag tctggggagc tattcactta
     8101 aaaatggcga aaaactggcg ggaagatcgg tactgggcaa ctttgttctg ggtacacggg
     8161 tgcccgatct cagcggaaaa ttccagctca gtattaccag cctgacccga aaacagttcc
     8221 tctcttttct gccgtccggc gaaaacttcc tgccactgac gatgtttgtg tccttcatcc
     8281 tgcgcgatca gcttgcctgg gacttacatc tggggctggc gccggagcag gtgggcgcaa
     8341 tgcgcctggg cgataacaaa agtgcgctgc tgggctggac cagcttcctc ggcacaccgg
     8401 aagaacgacc ctcagtcacc atcagggtac ggtcgtaaat tatgataatc acggagcaga
     8461 atatgccgga agagaaacta cagacgcttt cattgcaggt cattaacggc agtgagctgg
     8521 aaagcggacg ggcggcgcgc tgtctgttca cacagcaggg aaatgtcggc catggccccg
     8581 aatgccactg gtcggtacag gatcgtcagc agagcattcc ggcccaggct tttaccgtta
     8641 tcctgcacga tggcacattt tgtctgcgcc cgcagacggc acaactgtgg ctgaatcagg
     8701 caaaagtcac agcaacatca gacctaatac agttgcgcca gggcgatgag atccagatcg
     8761 gacggctgat ggtgagggtt catctgaacc ggggagatat tccccattac gatgaggaaa
     8821 tggccactcc cgaaaccatc gttaccaatc gcgatatgct cacggatacc ctgctatcaa
     8881 cggagggtac gccacactat ccgggaatga ctcaccggca ccaacttgca gacaccgtgg
     8941 taaacggttt ttctgccgat ccactccagg cacttcagtc cgaaagcctg attaccacgg
     9001 gcgatccgct ttcaggcatt gcggctgtcc ggccatcggc accgctgtcc gatccggcaa
     9061 gtaatggggg gatcaatact ccgtttatgg atctgccgcc catttatgcc agccccggcg
     9121 atcgtaatga cgacgtctct gcggcagaaa tggcgcaacg ccaccttgcg gtcaccccct
     9181 tactgcgcgg tcttggcggc tcgcttacca tgagcaattc cgacgatgcg gatgattttc
     9241 tggaggaggc cggacgaacg ttacaggccg caataaaagg tctgctcgat ttgcagcagc
     9301 agcgtaacag cctctcagac aaacatttgc gcccgctgga agataacccg ctgcgcctga
     9361 acatggatta cgccaccgcg ctcgacgtaa tgtttgccga aggtaaaagc ccggtacatc
     9421 tggcggctcc cgccgccgtc agtgaaagcc tgcgcaatgt ccgccaccac gaagaagcta
     9481 accgggcagc gattgtggag tcgcttcgtg tcctgctgga tgctttctca ccacaaaatc
     9541 tgctgcgccg ctttgtgcag taccgccgca gccatgaact gcgccagccg ctggatgatg
     9601 ccggagcatg gcaaatgtac agccattatt acgaagaact ggcctccgat cgccagcagg
     9661 ggtttgagat gctgtttaac gaggtctacg cccaggtcta tgaccgggtg cttcgtgaaa
     9721 aacagcggga gccggaagca tgaacaggaa ggcgtttctg gcctgcgtgc tgatgtgcgt
     9781 catattaacc ggctgcgaaa cggcgaaaaa aatcagtgag gtgatcaaaa acccggatat
     9841 ccaggtcggg agcctgaagg agcaaccttc ggaaataacc gtcaccctgc tgaccgaacc
     9901 ggacaccaat accaatgcgg aaggtgaatc agccgccgtg gatgtgcaac tggtgtacct
     9961 gactgacgac tcaaaattgc tggcagccga ctatgaccag atagccagta cgccgctacc
    10021 ggatgtgctg ggtaaaaact atatcgacca ccaggatttc aacctgttgc cggacaccat
    10081 caaaaccctg ccgccggtaa agctggatga gaaaacgcag tttattggtg tcgtggccta
    10141 tttctccgat gaccaggcca ctgaatggaa acaaatcgag acggtggaag gaaccggcca
    10201 ccattaccgc ctgctggtgc atgtacgtca aagcagcatc gaaatgaaaa aagaggatga
    10261 ataattatgg caaccacccg caacaaggtg atgtggcagg aagggatgct gatgcgccca
    10321 caccatttcc agcaacagca gcgttacaac gactacctgg ataaccagcg tttccgggcc
    10381 atgaatgatt tatcctgggg atttaccgaa ctcaccctca acaatgaact gctggcgcag
    10441 ggtaagatca tgattgacag cgcgtcaggc acactgcccg acggcaccgt cttttctatc
    10501 cccgaccagg acgcactgcc cgatccgctg cacccgcaaa attttccgga cgaaagaagc
    10561 cgcaatatct acctcgctct gccggtcgcc agtgatgtga gaaatgaaat cagcgacgga
    10621 cggcgaatcg ggcgttaccg gctgaattat gccgatgtcc gggatttgca ttcagaagaa
    10681 ggtgacgcgc gaacattgac gctgggtcaa ctgacgccgc gcattatgag cggtgcagaa
    10741 gatatgagcg cctatattac gctcccgctt tgccgtatca gcgatcgcca tgccgatggc
    10801 tccctgacgt tggatgacga ttttatcccc tcctgccaga atattcaggt cagtaagaaa
    10861 ctgcgtgttt atctcaaaga ggtacagggg gccattggcg gacgggcaag cgatctggca
    10921 aaccgtattg gctctccggc gcagagcggc atcgcggatg tggcggaatt tatgatgttg
    10981 cagttactta accgtaacca gacccggttt acccatcgcg cccgccgatc ccagctccac
    11041 ccggaagatt tctaccttga tcttgctggg ttgctgggtg aactgatgac ctttaccgag
    11101 ccgtcgcgcc tgccctgccc gcttgatgtg tatgatcatc atgatctgac caaaacattt
    11161 aaaacactgt taccggaagt caaacgggcg ctacataccg tactgtcgcc aagagcggtc
    11221 aatctgccgc tgcatctgcg tgacggtatc tggcaggccg atatccatga cacagaactg
    11281 ctgcaatctg ccacctttgt gctggccgtg gcggcaaacg tgccggtcga tcagatccag
    11341 cgtcagttta tccagcagtc gaaaatttcc tcgccggaaa aaatccgcaa tatggtcagt
    11401 gtgcagatcc ccggtattcc attgcgtgcc cttatggtgg ctccccgcca gcttccttac
    11461 cattccgggt tcagctattt cgaactcgac aagagtggcc aggcctggac agaaatggct
    11521 gccgccgggg ccgttgcact gcatgtatcc ggcagtttcc cggatctgaa catgcaactg
    11581 tgggcgataa gagggtaaga cgatggatga ggggtcactg tctctgccac catttaccgg
    11641 atacgacgaa aaaagccagc gcaactacca tctcgcgctt cgtggcaaca gcctgaatcc
    11701 gatgatcgac gcggccacgc cgctactcgg tatggtaatg cgcctttcaa ccatgaacag
    11761 ccagacgatg ccggagcatc tttttgctca ggtggtgaca gatgttcagg ccgtggagca
    11821 actgcttcag gagcagggct acgaaccggg cgtgattatc tcgtttcgct acatcctctg
    11881 cacgtttatt gatgaagcgg cgctcggtaa cggctggtca aacaagaacg agtggatcaa
    11941 gcagtcattg ctggtccatt tccacaacga agcctggggt ggcgagaaag ttttcatcct
    12001 gctggagcgc ctgatccgtg aacccaaacg ttaccaggat ctgctggaat ttctctggat
    12061 ctgtttttct ctgggatttc gcgggcgcta caaagtggca gcacaggatc agggggaatt
    12121 tgagcagatc taccgccgtc tgtaccacgt tctgcataaa ctgcgcggcg atgccccttt
    12181 cccgctgtta caccaggaca aaaaaaccca gggcgggcgt tatcagctca tcagtcgcct
    12241 gactgttaaa catatttttt gtggtggcgt tgtcgtgctg gcgttgtttt acctgttcta
    12301 tctgttgcgg ctggacagcc agacgcagga catcctgcac cagctaaaca aattacttcg
    12361 ataaggacgt ttatgatcca gattgatctt cccacgctgg taaaacggct gaacctgttc
    12421 tcccgccagg cgctggagat ggccgcctct gaatgtatga gtcagcaggc agcggaaatt
    12481 accgtcagcc atgtgctcat tcagatgctc accatgccac gcagtgacct gcgggttatt
    12541 acccgccagg gcgatattgg catggaagag ttgcgccagg cgctgacggt agagaactac
    12601 acaaccgccc gttctgcgga cagctacccg gcgttttccc cgatgctggt tgagtggctg
    12661 aaagagggct ggctgctggc gtcggctgag atgcagcaca gcgaactgcg cggcggcgtg
    12721 ttgctgctgg ccctgctgca ttcgccgctg cgttatatac cgcctgctgc cgcccggctg
    12781 ttgaccggca ttaaccgtga ccgtctgcaa caggactttg tgcagtggac acaggagtcg
    12841 gcggaatcag tcgtgccgga tgcagactgt aaaggcgcag gcgcactgac ggacgtcgct
    12901 gacagcctgc ttgcccgcta cgccaaaaac atgaccgaag atgcccgtaa cgacaggctc
    12961 gacccggtac tgtgccgcga ccacgaaatc gacctgatga tcgacattct ctgccgtcgc
    13021 cgtaaaaata acccggtggt ggtgggcgaa gcgggtgtgg gtaaaagcgc cctgattgaa
    13081 gggctggcgc tgcgtatcgt ggcaggccag gtgccggaca agctgaaaaa caccgatatc
    13141 atgacccttg acctgggcgc attgcaggcc ggggcatcgg tgaaaggtga gtttgagaaa
    13201 cgcttcaaag ggctgatggc ggaggtcatt tcctccccgg tgccggtcat tctgtttatc
    13261 gacgaagcgc atacccttat tggcgcgggc aaccagcagg gtgggctgga tatctccaac
    13321 ctgctcaagc cggcgctggc gcgcggcgag ctgaaaacca tcgccgccac cacctggagc
    13381 gagtacaaaa aatacttcga aaaagatgcc gccctgtcgc gccgattcca gttggtgaag
    13441 gtcagcgaac ccaacgctgc cgaagccact attattctgc gtggtctgtc ggcggtctat
    13501 gaacagtctc acggtgtgct gattgacgat gacgccttgc aggccgcagc aacactgagc
    13561 gagcgttatc tctccgggcg tcagttgccg gacaaagcca ttgatgtgct ggatactgcc
    13621 tgcgcccgcg tggcaatcaa cctttcatcg ccaccaaagc aaatctcggc gctgaccacc
    13681 ctgagtcacc agcaggaggc ggaaattcgc cagcttgagc gcgagcttcg catcgggctg
    13741 cgtaccaaca cctcgcgaat gacggaggtg ctggtgcagt atgatgaaac gctgacggcg
    13801 ctggatgaac tggaggccgc ctggcatcag caacagacgc tggtgcagga gattatcgcc
    13861 ctgcgccagc agttactggg tatggcagaa gacgatgcgg cgtcgttgcc gcacgtggat
    13921 gccgtggagg atacgccgcc agagtcagaa caggataata ccggtgctaa actggctgat
    13981 gaggccgaca gagaacaacc ggaagagacc gctgaaacag tttccccggt acaacgtctg
    14041 gcacagctca ctgccgaact ggacgccctg cataacgacc ggttgctggt ttccccgcac
    14101 gtcgataaaa aacagattgc ggcggtgatt gccgaatgga ccggcgtgcc gcttaaccgc
    14161 ctgtcgcaga atgaaatgtc ggtcatcacc gacctgacga aatggctggg tgacaccatc
    14221 aaaggccagg acctggcgat tgccagcctg cataagcatc tactgaccgc acgcgccgac
    14281 ctgcgtcgtc cgggacgccc gctgggcgcg ttcctgctgg ctggccccag cggcgtgggt
    14341 aaaactgaaa ccgtcctgca actggcagag ctgctctacg gcggtcgcca gtacctgacc
    14401 accatcaata tgtccgagtt ccaggagaaa cataccgtct cgcgactgat tggctcccct
    14461 ccgggctacg ttggctacgg tgaaggcggc gtactgaccg aagcgattcg ccagaaaccc
    14521 tactcggtag tactgctcga tgaagtggaa aaagcgcacc cggatgtgct caacctgttc
    14581 taccaggcgt tcgataaggg cgaaatggca gacggtgaag gccgcctgat tgactgcaaa
    14641 aatatcgtct tcttcctgac gtccaacctc ggctaccagg taatagtcga gcatgccgat
    14701 gacccggaaa ccatgcagga agtactgtat ccggtgctgg ccgacttctt caaacctgcc
    14761 ctgctggcgc gtatggaagt ggtgccgtat ctgccgctgt cgaaagagac gctcgccacc
    14821 attatcgccg gaaaactggc ccgtctggat aacgtgctgc gcagtcgctt tggtgcagaa
    14881 gtggtcattg aaccggaagt gacggacgaa atcatgagcc gcgtcacccg cgcggaaaac
    14941 ggcgcgagga tgctggaatc ggtcatcgat ggcgacatgc taccgccgct ctcgctgctg
    15001 ctgttgcaga aaatggcggc taacacggcg attgcccgga ttcggttgtc ggcagtggac
    15061 ggcgcattta cggcagatgt ggaagacgct ctggacgacg agtccgtcac agaggatgaa
    15121 ccggttttat gagcaggttg cgcgttttcc ggcagacagg atggtttatc gcgggcctga
    15181 tgacgggctt acccgccact gccgcgcccg cagaagcatc gtcaatggcc ggtgtggcgg
    15241 tcgccgtcgc caccacgacc ccgccggatg cgacagccac gttacaggcc atgcagtcct
    15301 gtcggcggga gtccgcggcg ctggagcggc ttgactgcta tgaccgcctg ctggcaccgc
    15361 tgtccccgtc agggtttgac ggggcgctgg tcaaagccgg ttttgtgggc gaggcctgga
    15421 cccgcgccac agagcaggaa aaacgccgcg agggcaacac cacagaactg ctggtgacgc
    15481 aggtgccggg ggagcgcccg acggtggtga taaccacccc ggctatcggt catgttccgc
    15541 cacgcccggt gctgatgttc agttgcgtgg acaatatcac ccggatgcag gtggcgctga
    15601 tgcaccctct ggatgtccat gatatcgccg tcactctgaa tgccgacaac cgggcattgc
    15661 gcagccactg gtttgtgcgc gagaacggca ccctgctgga gtccagccgg gggttatccg
    15721 ggattgatga aattaagcaa ctgttcggcg caaaaacgct gacggtggat accggcgcag
    15781 acaatgccgc cggaaagctg acctttaaca ttgacgggct ggcaagggcg attgctcccc
    15841 tgcgcgatgc ctgccactgg gcgggagaat aaaaatatgg caatggactt acgcgatccg
    15901 aatgtctggg tatcgcacct gctggaaaac ctgccggaag agaaactggc atcggcgctg
    15961 aaagatgaca acccgaactg ggagtatatc gacggcgaaa tcgtcaagct gggatcgctg
    16021 gcccacagtc aactggatat cccggaactc cagcgccggg ggctggtcat tctggcttcg
    16081 gagagtaagg attttcgtct gctggcgcat ctgctgagaa ccctgcaaca cgccggtgat
    16141 cctcatctcg cattgcgcct gctggcgctg tatgtggagc attactgggc ggtggctgcg
    16201 ccgcagaata tggcgcataa aaaacgcttt gccagccagg tcattaaacg ctttgaaaca
    16261 ggtattgaag ttttttcaca gaacgccgcc acagcgcagc gcgatgcgct gtcaggggaa
    16321 ctggcaaaac tggcgcaatg ctggcagtca cataacgccc cggaactggc acaggctact
    16381 gatgatcttt ttgccctgta ccagcgtgcg tttaatcgtg cggcaccggc tcctgtcccc
    16441 actccggcgg cctccggtag ttcaccacag accaccgcca cgtctgaaag cggcgtgacg
    16501 caacccagtg ctccggctcc ccaaatcgtc atcgacagtc acgacgacaa agcctggcgc
    16561 gacacgctgt taaaagtggc ggccatttta tgtgaacgcc agccggattc accgcagggc
    16621 taccgtctgc gccgccatgc gctttggcag aacatcacca gtacgcccca ggcggaaagc
    16681 gatggccgta ctccactggc tgcggtctcc gccgatatgg tggcagatta ccatgctcag
    16741 ctcggtagtg ccgatatggc gctgtggcag caggtggaga aaagcgtact gctggctcct
    16801 tactggctgg atggtcactg cctttctgca cagacggcgc tgcgtctggg ttacaaacag
    16861 gtggcagacg ccatccgcga tgaggtcatc cgcttccttg agcgtctgcc ccagcttacc
    16921 gggctgctgt ttaatgacca cacaccgttt atcagtgagc agacgaaaca atggctggca
    16981 gcttcgcccg acgccaaagt tgcaccggtt gcgcaaatcg gtgaggaatc gaaggcagcc
    17041 agagcctgtt ttgctgagca gggtctggag gcggcgctgc gatatctgga catgctaccc
    17101 gaaggcgatc cccgcgatca gtttcaccgc cagtaccttg ccgcacagtt gacggaagag
    17161 gcggggctgg tacagcttgc gcagcagcag taccggatgt tgttcaggat gggattgcag
    17221 atgatggtgg cagactggga gccgtccctg cttgagcagc tagaacaaaa attcacggca
    17281 gaacaataaa agagaacagg agtttttgtg ttcagattac caacaccccg actactcagc
    17341 ggactcaaat cagccctgcg accggcgatg ccccggttta aagtctctgc tttctggctg
    17401 ctgatactgg cgtggatttt tctgctggtg tggatctggt ggaaaggccc gacgtggacg
    17461 ctgtatgaag aacagtggct caaaccactg gcgaaccgct ggctggcaac ggcggcgtgg
    17521 gggattattg ccctgatgtg gcttaccgtc cgggtgatga agcgcctgca acagcttgaa
    17581 aaaatgcaaa agcaacagcg cgaggaagcc gttgatccgc tcagcgtgga actgaacgcc
    17641 cagcagcgtt atcttgaccg ctggctgctg cgcctgcaac gccatctcga caaccgccgt
    17701 ttcctgtggc agttgccgtg gtatatggtc atcggcccgg cgggcagtgg caaaacgaca
    17761 ctgctgcgcg aggggtttcc gtccgacatt atttatgccc cggagggcgc acgcggcgca
    17821 gaacaacgcc tgtacctcac gccccatgtc ggtaaacagg cggtgatctt tgatatcgac
    17881 ggcacactct gcgctcccgc tgatgcggat atcctgcatc gccgtctgtg ggaacatgcc
    17941 ctcggctggc tgaaagaaaa gcgcgcgcgc cagccgctga atgggattat tctgacactc
    18001 gatttacccg atctgcttac cgcagacaaa cgccgccgcg agcatctgtt acaggcgctg
    18061 cgcagccgct tgcaggatat acgccagcat cttcactgcc agttaccggt ttacgtggta
    18121 cttacccggc ttgatttatt gcagggtttt gccgccctgt ttcagtccct gaacagacag
    18181 gatcgcgatg cgattctggg cgtcacgttc acccgccgtg cccatgaaaa tgatgactgg
    18241 cgaacagagt tgaatgcttt ctggcagaca tgggtggatc gaatgaatct ggcgttgccg
    18301 gatctgatgg tcgctcagac tcacacccgc gcgtctttat tcagtttttc ccgccagatg
    18361 cagggaagcc gtgaaccgct ggtgtcactg cttgagggtc tgcttgatgg cgaaaatatg
    18421 aacgtgatgc tgcgtggtgt ctatctcacc tcttcgcttc agcgtggaca gatggatgat
    18481 atattcaccc agtctgccgc ccgccagtac cggctgggca ataacccact ggcgtcctgg
    18541 cccctggtgg acaccgcgcc ttatttcacc cgcagcctgt tcccgcaggc attactcgca
    18601 gagcctaatc tggcaacaga gagccgcgcc tggctgatac gttcccgtcg ccgcctgacg
    18661 gttttctctg ccacaggcgg cgtggcagca ctgctgctca tcaccggctg gcatcactat
    18721 tacaacggta actatcagtc tggcatcacc gtgcttaagc aggccaaagc ctttatggac
    18781 gtgccgcctc cgcaggggga agatgacttt ggcaacctgc aactgccgct cctgaaccca
    18841 gtacgcgatg ccacactggc ctatggcgac tggggcgacc gcagccgtct ggccgatatg
    18901 ggactgtacc agggacgacg tatcgggcct tatgtggaac agacctatct gcaactgctg
    18961 gagcaacgtt acctgccctc gctgtttaac gggctggtca aagcgatgaa cgccgcgccg
    19021 ccggagagtg aagaaaaact cgcggtgctg cgcgtaatgc gaatgctgga ggacaaaagc
    19081 ggacgtaaca atgaggtggt gaagcagtat atggcaaaac gctggagcga aaaattccac
    19141 ggccagcgcg atatccaggc acaactgatg tcccatcttg actacgcgct ggctcatact
    19201 gactggcatg cagagcgtca ggcgggtgac ggtgacgcca tcagccgctg gacgccatat
    19261 gacaagcccg tggtatcagc acagaaagaa ctgagcaaac tgcctgtcta ccagcgggtt
    19321 taccagagcc tgaaaacgcg ggcgctgggc gttcttcctg ccgacctcaa tctgcgtgac
    19381 caggtagggc caacctttga ccaggtgttt acatctgccg atgacaacaa actggttgtt
    19441 ccacagtttc ttacccgtta cggcctgcaa agctattttg taaaacagcg cgatgaactg
    19501 gttgaactga cggcgatgga ttcctgggta cttaacctta cccgcagcgt gaaatacagt
    19561 gacgccgacc gtgcggaaat ccagcgccag ttgaccgagc agtatatcag cgactacacc
    19621 gccacctggc gggccgggat ggacaatctg aatatccgca attttgagtc catcggacaa
    19681 ctgaccgggg cgctggagca ggttatcagc ggcgacctgc ctttgcagcg ggcgctaacc
    19741 gtgctgcgtg acaacacaca gccaggcgtc ttttctgaaa aactctctgc caaagaacgg
    19801 gaggaagccc tggcagagcc ggattaccag ttactcaccc gcctcgggca tgaattcgcc
    19861 ccggaaaaca gtaccctggc agtacagaaa gacaaagaaa gcacgatgca ggccgtgtat
    19921 cagcaactca ccgagttgca ccgctacctg ctggcaatcc agaacgcgcc tgtaccaggg
    19981 aaatcggcgc tgaaagccgt gcagttacgg cttgatcaga acagcagcga tccgatattc
    20041 gccacccgcc agatggcaaa aacgctgcct gctccgctca accgctgggt tggcaggctg
    20101 gctgaccagg cctggcatgt ggtgatggtg gaggctgttc attatatgga agtggactgg
    20161 cgcgacagcg tggtgaaacc gtttaacgag caactggcaa ataactatcc gtttaatccg
    20221 cgttctgcac aggatgcctc actggatgcc ttcgaacgct tctttaaacc ggatggcata
    20281 ctggatacct tctaccagca gaacctgaag ctgtttatcg ataatgacct gagtctggag
    20341 gatggcgata acagcgtcat tattcgcgaa gatattattg cgcaactgaa aaccgcgcag
    20401 aaaatccgtg acattttctt cagtaaacag aacggtctgg gaacatcctt tgccgtggaa
    20461 acggtatcgc tctccggcaa caaacgccgc agcgtactga accttgacgg ccagttagtc
    20521 gattacagcc aggggcgcaa ctataccgcc catctggtct ggcctaacaa tatgcgcgaa
    20581 ggcaacgaaa gcaaactgac gctggttggt gccaacggcg gcgcaccgcg cagtatcagc
    20641 ttcagcgggc cgtgggcgca gttccgtctg ttcggggccg gacaactgac cggagtacag
    20701 gatggcaact ttaccgtgcg ctttagcgtg gacggtggcg cgatgaccta ccgtgtgcat
    20761 accgacacgg aagataaccc gttcagcggt ggtttgttca gccagtttgg tctgtcagac
    20821 acactgtact gaggaggcag ggatgaatag taacggactg acacaaacta tcgttaccgg
    20881 cagtgacccg cgcgggctgc cggaattcag cgccatccgc gaagaaatta acaaagccag
    20941 ccacccgtca cagcctgagc tgaactggaa actggtggag tcgctggcgc tggcgatttt
    21001 taaagccaac ggagtggatt tacacaccgc cacctactat acgcttgccc ggacacggac
    21061 ccagggactg gcgggattct gcgaaggtgc ggaactgctg gcggcaatgg taagccacga
    21121 ctgggataag ttctggccgc agggcggccc ggcgcgtact gaaatgctgg actggtttaa
    21181 ctcccgaacc ggcaatattc tgcgtcagca aatctccttt gcggaatccg acctaccact
    21241 gatctaccgc acagagcggg cattgcagct tatctgcgat aagctccagc aggtggaact
    21301 gaagcgcgtt ccgcgcgtgg agaatctgct ctattttatg cagaacacgc gtaaacggct
    21361 tgaaccccag ctgaagagta acactgagaa cgccgcacag accacggtca gaacgctgat
    21421 ttatgtcccg gaaacacagg catcttccac accagaagcg gtagtgcctc ccctgcccgg
    21481 cctacctgag atgaaagtgg aagtgcgcag tctgacagag aatcccccac aggccagtgt
    21541 gataaagcaa ggcagtacgg taagagggtt tatcgcaggg atcgcctgtt cagtggctgt
    21601 cgcctcagca ttgtggtggt ggcaggtcta tccggtgcag cagcaactgt tacaggttaa
    21661 cgacaccgct cagggcgcag caacggtgtg gatggcctca cctgaactcg aaaactatga
    21721 gcgcaggctg caacaacttc ttgatacctc cccggtacag ccgctggaaa ccgggatgca
    21781 gatgatgcgt gttgccgaca gtcgctggcc ggaaagcctg caacagcaac aggcctcgac
    21841 acaatggaat gaggcactca aaacccgcgc acagagtagc ccgcagttgc gtggctggtt
    21901 gcagacccgc caggacttac atgcttttgc agatctagtg atgcagcgcg agaaagaggg
    21961 actaaccctt tcctatatca aaaatgtcat ctggcaggcg gagcggggac tggggcagga
    22021 aacacccgtt gagtctctgt tgacgcagta ccatgatgcc cgtgcgcaga agcagaatac
    22081 agatgcgctg gaaaaacaaa ttaatgagcg actcgaaggc gtgttaagcc gctggctgct
    22141 gctgaagaat aacgtcatgc cagaggcggc aaccggtacc acggccgaaa aataattatt
    22201 taattaaagg agattatcat ggcaaatata agttatttat ctttgagtgg agagacacaa
    22261 gggctaattt cagcaggttg ctcaacgctt gattctgtcg gaaacaaagc gcagccagaa
    22321 cataaagatc agatcatggt ttatgcgtta atgcattcca tctcaagaag tcaaaatgtc
    22381 aatcatcatg aattaattat caccaaaccg gttgataaat cctcaccatt gctggcaaaa
    22441 gcacttagtg acaatgaaaa aatggcaata tgtgaattta tactctaccg aaccagtaaa
    22501 gcgggtattt accagcctta ttataaaata aatctgtcaa aagcccgaat atcaagtatt
    22561 gattttgtca caccgcatgc tgtcctggaa aaagaactgg aaccacagga gagaattgcc
    22621 tttatttatg aagatatttc ctgggagcat acgttagccg gtacgaatgc aatgagtaaa
    22681 tggcaggatc gtgtacagta acttactgta ataacataat aaattactat tgcggagaga
    22741 taaaatggct ggtttaagga ataacaacaa cacacagaat gcacagtggg ctgattatgt
    22801 gggtgacatt cttcgggggg ctcagccgat aaaccagcta gtacctcagc acccttatct
    22861 taacgacgtc ccactcattg atgagttaag gcatcagaac actcatcatg ttattctctt
    22921 gacacttgat gttgcaaaaa aaatactcag cccaataaca tcatttgatt acattcattt
    22981 tatcactaca catccttcag gtataaaaga tacactcgcc tggttagtta acgcgagtaa
    23041 attaatgact gagtttgatg acaatgggaa aattatattt aaccttaatg ccttaaagta
    23101 tacaaaagca agttactttg aaatacttgg agagaaatat ataaaaatca caacatccag
    23161 cccctggctt ttagaaaagc tcggtaaata tattttctct tcacgagcgc ctcaagttct
    23221 ggaactcgct attggctggc gaggagcttt atctgaatca ataaaaggag ttaagttctg
    23281 tatctggttt tcagtagcct ggagaaccat agaatttatt atgagttcag agcgagatct
    23341 ggttaatttt cttggcgatt tttcaatgga tgtggccaaa gcagttatag ccggtggtgt
    23401 tgcaaccgct attggaagcc tggcttcttt tgcctgtgtt agctttggct ttccagtaat
    23461 tcttgtcgga ggagcaattt tactgacagg gatagtgtgt acagttgttt taaatgaaat
    23521 cgatgctcaa tgccatttat cagaaaaatt aaaatatgca attagagatg gactaaaacg
    23581 gcaacaggaa cttgataaat ggaaaaggga aaacatgact ccatttatgt atgttcttaa
    23641 cactccaccc gtgatatgag gattaaccat gcgtttacat gttaaattaa aagagtttct
    23701 ttcgatgttt tttatggcca tattattttt ccctgcattc aatgcttctt tgttttttac
    23761 cggagttaaa cctctatatt caataataaa atgctccacg gaaatatttt atgattggag
    23821 aatgttgatt ctgtgctttg gtttcatgtc gttttccttt ttaaatgttc atgtaatttt
    23881 acttacgata ataaaatcat tcttaatcaa aaaaacgaag gtagtcaatt tcgccacaga
    23941 cataacaata cagctaactc ttatattttt actaatagct atcgtaatag cacctttaat
    24001 agctcctttt gtcacaggat atgtcaatgc taattatcac ccctgcggta ataatacggg
    24061 aattttccca ggagcaatct atattaaaaa tggcatgaaa tgtaataatg aatatatttc
    24121 tcgcaaggaa gattcagcag ttaaataatt aaatattcta aaacaaacag tttttaactc
    24181 cggattccat gtcctttctt gcactgtctt tgtcaagaaa aatttcttat gcaaagaatt
    24241 ttgtattatt gccgccagaa attcctggcg gcagatataa tttattattg atagcggtta
    24301 accatatctt ttaccatcgc tttataggtc ttttgcttaa gttgcatgat ttgctgctct
    24361 gtcatatcag tcgtactggc ttcattcttg tcgggcaatg gtggaagcac tttatattca
    24421 taattcaccg gcgaccaggt agcccactca cctgttgcca catccaccag cgtgaaacgt
    24481 accaggtagc gcagggagat agcgtcggtg agtttttcat ttttatagtc ggaccatact
    24541 gttgatttca gcgcagagtc atattttcct gtctgcaatg tatcctggta aacaataatc
    24601 gctttctgat gtccttttgc agccacaaaa cgcagtgcct gcatccagtt catattctca
    24661 gcactggcaa catcctcgtt ctcattttta tctttgtttt tgttacaagt cagaggcttt
    24721 tgcctgtccg ggataccaga gaatgtggaa acagtataat attttcgcat ctcctcctgc
    24781 ataatggttt ccggtgcgcg gttgccggat tgaacaagta ttacaggaga attaagggga
    24841 acgcggaact catctccatc aagcgcagct tgaatatcct cttcggatac ggccagagtg
    24901 gtttcattac cgaataaatc tttgtttgtc agttgctggg gagtgttttt attaatatta
    24961 ctttgtgtga cttgagcttt tttcttcttt tcatcaatag aagattcaga ggcacatcct
    25021 gcaagcagcg agaataacac acatatgcaa caaagctttt ttgaattcat aattggacac
    25081 tccctcgcct gtcataatga ttagaatatt tagcattgat aacggactct aattatatac
    25141 cccacttaaa taataacaaa caaaatcaat ttgaaatata cattcattta atcaatataa
    25201 atttattctc tggaaatata tttaagctga atggttcgat atcattattg aattttaatg
    25261 aatgagacaa caagcgagaa tcacgcactt acgccagaat aaaatcattg aacagagaaa
    25321 tggtggaatt caaatacaaa aaaaccgcaa aattaaaaat cttgcggctc tctgaactca
    25381 ttttcatgag tgaatttggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca
    25441 ggcaggtatt ctaaccgact gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg
    25501 aatattacgg attgcctcac ccttcgtcaa cggtttttct catcttttga atcgtttgct
    25561 gcaaaaatcg cccaagccgc tatttttagc gccttttaca ggtatttatg cccgccagag
    25621 gcagcttccg cccttcttct ccaccagatc aagacgggct tcatgagctg caagctcttc
    25681 atctgtcgca aaaacaacgc gtaacttact tgcctgacgt acaatgcgct gaattgttgc
    25741 ttcaccttgt tgctgctgtg tctctccttc catcgcaaaa gccatcgacg tttgaccacc
    25801 ggtcatcgcc agataaactt ccgcaaggat ctgtgcatcg agtaatgccc cgtgtagcgt
    25861 tcgtttactg ttatctattt cgtagcgggc acataacgca tcgaggctgt tgcgcttacc
    25921 aggaaacatt ttcctcgcca ccgcaaggct atcggtgacc ttacagaagg tattggtctt
    25981 cggaatatcg cgcttaagca acgaaaactc gtagtccata aagccgatat cgaacgctgc
    26041 gttatggatc accaactccg cgccgcggat atagtccatg aactcatcgg ctacttcggc
    26101 aaacgtgggc ttatcgagca aaaattcatc ggcaatacca tgtacgccaa aggcttccgg
    26161 atccaccagc cgatcgggtt tgagataaac atggaagtta ttgcccgtca ggcgacggtt
    26221 caccacttca acggcaccaa tctcaatgat cttgtggcct tcatagtgcg caccaatctg
    26281 gttcataccg gtggtttcgg tatcgagaac gatctggcgt gtaattgcag tgctcatagc
    26341 ggtcatttat gtcagacttg tcgttttaca gttcgattca attacaggaa gtctaccaga
    26401 gatgcttaaa caggtagaaa ttttcaccga tggttcgtgt ctgggtaatc ctggacctgg
    26461 gggttacggc gctattttac gctatcgcgg acgcgagaaa acctttagcg caggctacac
    26521 ccgcaccacc aacaaccgta tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa
    26581 agaacattgc gaagtcattt tgagtaccga cagccagtat gtccgccagg ggatcactca
    26641 gtggatccat aactggaaaa aacgtggctg gaaaacagca gacaaaaaac cagtaaaaaa
    26701 tgtcgatctc tggcaacgtc ttgatgctgc attggggcag catcaaatca aatgggaatg
    26761 ggttaaaggc catgccggac acccggaaaa cgaacgttgc gatgaactgg ctcgtgccgc
    26821 agcgatgaat cccacactgg aagatacggg ctatcaagtc gaagtttaag cctgtggttt
    26881 acgacattgc cgggtggctc caaccgcctg gcgaattcgt ggcttgtttt tactctgttt
    26941 catcgggttt agcgttaaag gaatagtccg tttccgggca acaataagtt gtaagcaacc
    27001 aagcgcagga atatgcgcat tcaatagttt tcctccgtgt ttgttccacg ggagaacgtg
    27061 gaaacggctg gcgtgcagca cttcaaaatt caacaaagag agccagtcca gttgccgcat
    27121 cagagtaaac atccggctgt tatagggcga ggtttttcgc aataccggca caagtttgcg
    27181 taatcccatt aaactgatgg gattgaagcc actaatcacc agccagccat catcaatcaa
    27241 tacccgatcg gcttcacgca ataaacgatg cggatcggtg caccacggca atgtatgtgc
    27301 cagtagacaa acatcaacgg atttatcggc aaaaggaaga tgaagtgggt ccgcctgtac
    27361 ctggacgggc attccttgcg cagaaacatt cacttgatga gaaaccgcgc acgcttcgca
    27421 attgatttct gcgcttaaat tgccaatctt aagcagatga aaaccataca ttttagtgaa
    27481 ccacgggttg agctggcgct ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc
    27541 ccagcaatca ggagccacga cagtttgagg gactcttgcc ggtttcatca caaccttccg
    27601 tttcacactg agaggtaatc tatgaatctt aacagtattc ccgcctttga tgacaattac
    27661 atctgggttt tgaatgatga agcaggtcgc tgcctgattg tcgatcccgg agacgcagag
    27721 ccagtattaa acgccattgc cgccaataac tggcaaccag aggccatatt tctcacccac
    27781 catcaccacg atcacgttgg cggcgtaaaa gaactggtgg aaaagtttcc gcaaattgtg
    27841 gtgtatggtc cacaagagac acaagataag ggaacaacac aggtagtcaa agatggcgaa
    27901 actgccttcg ttttggggca tgaatttagt gtaattgcca caccgggtca cactttagga
    27961 catatctgtt acttcagcaa accttatcta ttttgcggcg acacgctgtt ttctggtggg
    28021 tgtggtcggt tgtttgaagg gacagcatca caaatgtatc aatcacttaa aaagttaagt
    28081 gcgttacctg acgatacatt ggtatgttgt gctcatgaat ataccttatc aaatatgaag
    28141 tttgctttga gtattcttcc gcacgatttg tccataaatg attattatcg taaagttaag
    28201 gagttacggg caaaaaatca aataacacta cccgtaattc tgaaaaatga gcggcaaatt
    28261 aatgtttttt taagaacgga agatattgat ttaattaatg taattaatga agaaacatta
    28321 ttgcaacaac ctgaagagcg ttttgcatgg ttaaggtcaa agaaagatag gttctgataa
    28381 aactttcttg tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa gcaactattg
    28441 acacacacat gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg ggttgccaga
    28501 gtaccggcaa cgttcaacag cacgcacaga gcctttctgc agctggtcaa ggggaagcag
    28561 caaagtttac aagtcaggca cgatggatgg acgacgggac gtctatcgcg ccagatggtg
    28621 acttgtgggc tttcattggc gacgagctaa agatgggaat tccggaaaat gaccggattc
    28681 gcgaacagaa acagaaatat ttacgcaata agagctatct ccacgatgta actttacggg
    28741 cagagccgta tatgtactgg atagcagggc aagttaaaaa acgtaacatg cctatggaac
    28801 tggtactact acccatagtg gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg
    28861 ccgcaggcat ctggcagatc attccgagca cggggcgcaa ttatggtttg aaacagaccc
    28921 gcaattatga cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg aatatgatgc
    28981 agcgtctgaa caaaatgttt gacggcgact ggcttctgac cgtagcggct tataacagcg
    29041 gcgaaggtcg ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact
    29101 tctggtcgtt accgttgccg caggaaacga agcagtacgt gcctaaaatg ctggcattga
    29161 gtgatattct caaaaacagc aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc
    29221 gtgctctggc gcgtgtgcac ctgagtagcc cggttgaaat ggcgaaggtt gcagatatgg
    29281 cggggatttc cgtcagcaag ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg
    29341 gcgcaagtgg gccgcagtac gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat
    29401 ctctcgcttc aggcgaaatt gctgctgtac agtcgacgct ggttgccgac aatacgccgc
    29461 ttaacagccg tgtttacacc gtacgctctg gcgacacgct ttcaagcatc gcttcacgtc
    29521 tcggcgtaag caccaaagat ttgcagcagt ggaacaaact gcgcggatct aagctgaagc
    29581 caggccaaag tttgacgatt ggtgcaggca gtagcgcaca gcggttggca aacaacagcg
    29641 atagcattac gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct aaacgccacg
    29701 gcgtgaacat caaagatgtg atgcgctgga acagcgacac tgcgaatctg caaccaggcg
    29761 acaagctgac gttgtttgtg aaaaacaaca acatgccaga ttcctgacaa accagataat
    29821 aaaaaggcac cgattccccc ggtgcctttt ttatttatgc cgctttatgt gcttctacca
    29881 tgatgatatc actggtgaaa gagccatcag tctgcaagga aaaatacgtt ctcacctctg
    29941 tcgatgcgct ctgttggtaa atgcgaatag cgtctactaa cgcttctggc gtacgcattc
    30001 tcacgaccca tgaagaaaat tccagcggta acttatctgt aattaaatta tcaactatca
    30061 gattggcttc attgattaac gtcaaccact caccgctggc gtagtttcgt acgtgagagg
    30121 tatcgcgtaa tgcttctacc gtctgtaacc agatgtcgcg cactgggtgg cccggagaca
    30181 ttacgtccat cacaatcagc ctaccgccag gtttcaatat ccggttcact tcacgcagtg
    30241 ctgcaccaac atcatgccag tgatgggcag aataacggct gataacaata tcaaatgcgt
    30301 tatcggcaaa tggcagactt tcggcataac cctggcgggt ggtgatattt ttcagttgcc
    30361 gggcttcggc agcttgtgcc acgacgccca gcatgtgcgc agataagtca tacgccacca
    30421 ccgagctcac gttttgcgca gcgacaaagc tagcatgccc tgctccgcag cccatatcaa
    30481 gtacacttgc accaggataa tcagccagac gcaccgccag gcgctgtaaa tctcgtccgg
    30541 atgcatgtac ggtactggtc agatattcgc tggcttgaga gctaaactgc ttttctacgt
    30601 ggtcatggtg ggattgtgtt gtcattgcgc tgtccttttg ttatttgagt ataaaagggc
    30661 agcgccaaac tttggtctgc cctgtggcag acctgacata ccttatttat caggcttgcc
    30721 gggactgaat tcaacgagta gcggattgtg atcggaagcg cgcgtaacca gtacagaagc
    30781 ttcgctgacg ttcagaccac ggtagaaaac aaaatcgagc ggacggccaa acgcccgacg
    30841 gcgctgatca tcggtaaaac gcacctggcg cagcgacatt tcccgcgcaa agcgatataa
    30901 cgcgttcatc cttctacggc tccaggcatt gaaatctccc gccataatga ccgggccgct
    30961 gtggtgagct atctgatcac caataggaag taactgctta ctatagacat ccacgcccag
    31021 actgaagttg acggcgtgta tattaaccac catcaacagg cgggtgtcag gtaatggata
    31081 gaccgtcacc agtgccgact tcgccagacg caaaatgggt tctcgttcac gtaacgggca
    31141 gcaatacact ggatgtgccg ccgaaagggt cattacgcca gaaggatgtt gtggcagcac
    31201 gaaagcgggt acctgatcgg cggcaagata gttagcggtc gcaaactgta ctaactctgg
    31261 cgttgtttgc gcttcctgca ataacaccag atgtgcgtct ttgccgtaat tctttaatac
    31321 cgacaaccat tcagcgcgtt gctgtttgta tatgttccac accaggatcc gaatacgctc
    31381 ttcggtactt aacggttccc caggtggtaa tgcctggccg atgctcgcaa aagaccccgg
    31441 cggtaagatc ctctccgcag gttgtccggc aacatagcgc atggcatagg tgttttttcg
    31501 cacttttgac tttgaaacct ctattacgtc aacccttccg cactcgggag ggttctactg
    31561 ttatagggat tttagctcac tctttcaacg agcaattgta tattgttatg taagcaagtg
    31621 cttacaccgg tcagaatttt gctcattgtc gagaacatta aatcggatga catatcacgt
    31681 atcctgatgg tttaatgaca aataaattca aaatatctct tctattttgc aacaggagca
    31741 aaaatgaaag ccacgtcgga agaactcgcc atttttgttt cggtcgtcga aagcggcagc
    31801 tttagccggg cagcggaaca attagggcaa gcaaactcag cggtaagccg ggcggtgaaa
    31861 aagctggaga tgaaacttgg cgttagcctg cttaatcgga ccacgcgaca acttagcctg
    31921 acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga gatggcagcg
    31981 gcagaatcag aaattatgga gacgcgtaat acaccgcgtg gactgttgcg catcgatgcc
    32041 gcaactccag tggtgctgca ctttctgatg ccgttaatta agcctttccg tgaacgctat
    32101 ccggaagtca ctttatcgct agtctcctcc gaaacgatta ttaatttgat cgaaagaaaa
    32161 gtggatgtcg cgatacgcgc tggtacgtta acggactcca gcttacgtgc caggccgtta
    32221 tttaacagtt atcgaaaaat tatcgcctcc cccgattata tttcccgcta cgggaagcca
    32281 gaaacgatcg acgatttaaa gcaacatgtt tgcctgggat tcactgaacc cgcttccctc
    32341 aatacctggc cgatagcctg tagtgatgga caattacatg aggtgaagta cggtttgtca
    32401 tccaatagtg gggaaacact gaaacagctt tgcctgagtg gcaacgggat tgcgtgtttg
    32461 tccgactaca tgatcgacaa agaaatcgct cgcggagaat tggtggagtt aatggcagat
    32521 aaagtgttgc cagtggaaat gccattcagt gccgtctatt acagcgaccg tgcggtaagt
    32581 acgcgcatcc gggcttttat cgatttcctt agcgagcatg taaaaacagc tcccggagga
    32641 gctgtcagag aggcttaatc ccattcagga gccagacctt ccgggctaac caggcggtcg
    32701 ttgcaatcca gtgcggcgat cgctttttta tcttcggcat caagctgtaa attttgtgcc
    32761 ttaagattac tttccaggtt tttacgttta gtagaagaag gaattactga gtaaccttcc
    32821 cccatagccc acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc tgcaatacga
    32881 gcaataacct catctttcag tgccttacca taagccagcg tcatatagga agtgatatga
    32941 ataccgtgct gtttagccca ggcaaccact ttacggtttt gcagataagg agagagttca
    33001 atctggttgg tagcgatgtt ttcagcgcca acagcagcaa tcgctttttc catcaacggg
    33061 atcgtgaagt tagaaatacc gatctcacgc gtcagccctt gttttttggc ttccagcagc
    33121 gcctgcataa actcttcaac agagacttca tcgtttggtg acggccagtg gattagcgtc
    33181 agatcaacat aatcggtacg caatttttgc aggctctctt tcagacttgg gatcaatttg
    33241 tctttgctga gattttcaat ccagatttta gtggtgatgt agagttcatg acgtggcacg
    33301 ccactttctg caatcgcctg acctactgcg gcttcgttat catagatttg tgcggtatca
    33361 attgtgcgat aaccaagttc aagcgccgtt ttcacagatg aaataacaac gtcgtctttc
    33421 agacggaaag tacctaaacc aaatgcaggg atagccataa tatgcctctt ttaatgctat
    33481 tttcgttaac tgaggtgatt atgggcttca gactgttgcg gaaaaagagc gtaaaacgca
    33541 gaggattttt gcgattctgg caataataga tatccagacg aaaaaaaacc tcgagcgtta
    33601 actcgaggct tcttaataag tggcggaacg gacgggactc gaacccgcga ccccctgcgt
    33661 gacaggcagg tattctaacc gactgaacta ccgctccacc gaattctttt acaaccaccg
    33721 gttttatgac cggcttactg cttaatttga tgcctggcag tttatgaatc acttcgtgat
    33781 tcacccttcg ggccgttgct tcgcaacgtt caaaagcttt cgcttttgtc ctactctctt
    33841 atcgagtagt gaactgcctc aaaggtcagt
//
LOCUS       DAEVZA010000039        32928 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEVZA010000039 DAEVZA010000000
VERSION     DAEVZA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.039"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            216..392
                     /locus_tag="LAF92_001963"
     CDS             216..392
                     /locus_tag="LAF92_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HBI9790979.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1020..1319
                     /locus_tag="LAF92_001964"
     CDS             1020..1319
                     /locus_tag="LAF92_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9790980.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVLPVTNIVLFLILIFFKKR"
     gene            complement(1366..2253)
                     /gene="rarD"
                     /locus_tag="LAF92_001965"
     CDS             complement(1366..2253)
                     /gene="rarD"
                     /locus_tag="LAF92_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBI9790981.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2305..2772)
                     /locus_tag="LAF92_001966"
     CDS             complement(2305..2772)
                     /locus_tag="LAF92_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBI9790982.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2937..3806
                     /gene="pldA"
                     /locus_tag="LAF92_001967"
     CDS             2937..3806
                     /gene="pldA"
                     /locus_tag="LAF92_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBI9790983.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3939..5768
                     /gene="recQ"
                     /locus_tag="LAF92_001968"
     CDS             3939..5768
                     /gene="recQ"
                     /locus_tag="LAF92_001968"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBI9790984.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5832..6452
                     /gene="rhtC"
                     /locus_tag="LAF92_001969"
     CDS             5832..6452
                     /gene="rhtC"
                     /locus_tag="LAF92_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBI9790985.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6514..7134)
                     /gene="rhtB"
                     /locus_tag="LAF92_001970"
     CDS             complement(6514..7134)
                     /gene="rhtB"
                     /locus_tag="LAF92_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBI9790986.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7245..8267
                     /gene="pldB"
                     /locus_tag="LAF92_001971"
     CDS             7245..8267
                     /gene="pldB"
                     /locus_tag="LAF92_001971"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBI9790987.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8275..9075
                     /gene="yigL"
                     /locus_tag="LAF92_001972"
     CDS             8275..9075
                     /gene="yigL"
                     /locus_tag="LAF92_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBI9790988.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9151..10050
                     /gene="bioP"
                     /locus_tag="LAF92_001973"
     CDS             9151..10050
                     /gene="bioP"
                     /locus_tag="LAF92_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBI9790989.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9938..10891)
                     /gene="metR"
                     /locus_tag="LAF92_001974"
     CDS             complement(9938..10891)
                     /gene="metR"
                     /locus_tag="LAF92_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBI9790990.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11009..13270
                     /gene="metE"
                     /locus_tag="LAF92_001975"
     CDS             11009..13270
                     /gene="metE"
                     /locus_tag="LAF92_001975"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBI9790991.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIESLLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(13309..14124)
                     /locus_tag="LAF92_001976"
     CDS             complement(13309..14124)
                     /locus_tag="LAF92_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBI9790992.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            14386..15147
                     /gene="udp"
                     /locus_tag="LAF92_001977"
     CDS             14386..15147
                     /gene="udp"
                     /locus_tag="LAF92_001977"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBI9790993.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            15287..16714
                     /gene="rmuC"
                     /locus_tag="LAF92_001978"
     CDS             15287..16714
                     /gene="rmuC"
                     /locus_tag="LAF92_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBI9790994.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            16809..17564
                     /gene="ubiE"
                     /locus_tag="LAF92_001979"
     CDS             16809..17564
                     /gene="ubiE"
                     /locus_tag="LAF92_001979"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBI9790995.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            17578..18183
                     /gene="ubiJ"
                     /locus_tag="LAF92_001980"
     CDS             17578..18183
                     /gene="ubiJ"
                     /locus_tag="LAF92_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBI9790996.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18180..19820
                     /gene="ubiB"
                     /locus_tag="LAF92_001981"
     CDS             18180..19820
                     /gene="ubiB"
                     /locus_tag="LAF92_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBI9790997.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19899..20168
                     /gene="tatA"
                     /locus_tag="LAF92_001982"
     CDS             19899..20168
                     /gene="tatA"
                     /locus_tag="LAF92_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBI9790998.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20172..20687
                     /gene="tatB"
                     /locus_tag="LAF92_001983"
     CDS             20172..20687
                     /gene="tatB"
                     /locus_tag="LAF92_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBI9790999.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            20690..21466
                     /gene="tatC"
                     /locus_tag="LAF92_001984"
     CDS             20690..21466
                     /gene="tatC"
                     /locus_tag="LAF92_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBI9791000.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            21508..22290
                     /gene="tatD"
                     /locus_tag="LAF92_001985"
     CDS             21508..22290
                     /gene="tatD"
                     /locus_tag="LAF92_001985"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBI9791001.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22287..22775)
                     /gene="rfaH"
                     /locus_tag="LAF92_001986"
     CDS             complement(22287..22775)
                     /gene="rfaH"
                     /locus_tag="LAF92_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBI9791002.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22942..24435
                     /gene="ubiD"
                     /locus_tag="LAF92_001987"
     CDS             22942..24435
                     /gene="ubiD"
                     /locus_tag="LAF92_001987"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBI9791003.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            24481..25182
                     /gene="fre"
                     /locus_tag="LAF92_001988"
     CDS             24481..25182
                     /gene="fre"
                     /locus_tag="LAF92_001988"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBI9791004.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(25374..26537)
                     /gene="fadA"
                     /locus_tag="LAF92_001989"
     CDS             complement(25374..26537)
                     /gene="fadA"
                     /locus_tag="LAF92_001989"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBI9791005.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(26547..28736)
                     /gene="fadB"
                     /locus_tag="LAF92_001990"
     CDS             complement(26547..28736)
                     /gene="fadB"
                     /locus_tag="LAF92_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBI9791006.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPNTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSLLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEESIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28926..30257
                     /gene="pepQ"
                     /locus_tag="LAF92_001991"
     CDS             28926..30257
                     /gene="pepQ"
                     /locus_tag="LAF92_001991"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBI9791007.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            30257..30871
                     /locus_tag="LAF92_001992"
     CDS             30257..30871
                     /locus_tag="LAF92_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBI9791008.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30910..32361
                     /gene="trkH"
                     /locus_tag="LAF92_001993"
     CDS             30910..32361
                     /gene="trkH"
                     /locus_tag="LAF92_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBI9791009.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            32373..32918
                     /gene="hemG"
                     /locus_tag="LAF92_001994"
     CDS             32373..32918
                     /gene="hemG"
                     /locus_tag="LAF92_001994"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBI9791010.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7717 a         8256 c         8934 g         8021 t
ORIGIN      
        1 ctaatagttt cgaaaagtgt acagagaata ttaattggcg gaagtaatat gttattcatt
       61 tgcatttgcc ttggttatat ttaagctcaa tgtcaattcg agaacaaatg ctagggtaaa
      121 aaaatattta aaaaagaagt acatttcaca ttttttcatt acatctctga agagaacctc
      181 gttaatgatt ggtcttgctc attgttaata acaaaatgtt gaatttaccg attaggaaac
      241 tttttcctac gcggattgcc tccagctatg gcatgaactt tgagtttatg ccagaactga
      301 agtggagctt tggctaccct ggcgcgatta tctttatgat ccttgcgggc ctgacaccgt
      361 atctgtactt taagcgtaag aactggctgt aactatacga tgtcgctgtg agtaagttag
      421 tgtgctgaca gcgacatttt agccgaggga atcgcagata atataattgt tgtgaataat
      481 atccatgcca cgttgagttg gacgcttaat gactcacatc tcaccccttc aattgctgca
      541 gagataatag aaaaaagtgt caaacccaat gaaatcaata caaagtagat tgccttacat
      601 ccagtatctc ttattctttt tgctccaata ttgatattta cataggtatt attaataacc
      661 agaataaaac tcaaagccag gttgatatca ttcaaagtta ttaatattga ccatgatgtt
      721 aatgtataga ttatgaactg aaagcgtgat aaatgctcaa gcctggatgg gtagaatata
      781 tttctgatgg atgatttgaa tattttttac attgtttttt atatataatt gttttgaata
      841 tatatattac ctgaattatg aggtttgcat atatatatgc aatatagcag tctttgcatt
      901 gataactata cagtagtaat ttagagtgtg taagttttta ttgatggggt atattttaat
      961 taaatataat tggaatgtat ttttattcta taaacaatat cttggggaat ggattctata
     1021 tggggaatgg taacttttgg cagttgttct tgggtaaaga accttacagc aaaaaatctg
     1081 attttgttaa ggcatggtta ttgtggtggg ctattaccat ccttgttcta tttattttaa
     1141 gcctgactac gacattaaat ttaagaagta taggtatagg gttaagtttt gggttttgtc
     1201 aaatccttgg tagatatacg atgtctctag gatggagtaa gtactgggga gttttggggg
     1261 tacttcctgt aacaaatatt gttctttttc ttatcttaat ttttttcaaa aaaagataaa
     1321 taagtcagtg ggtaggctca ttaaacggcc tacccatgtt aattcttaac tttttctacg
     1381 ctgcgtataa atcgcatcca tcacaaaaat tgccagcgcc acccaaataa aggcgaaagt
     1441 caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag
     1501 cgtcgggcca atgtactgga aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc
     1561 agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag
     1621 cgacatcggg ttttgcccca tatggctggt tgagctgtcg gcaatagcaa acaggtaaat
     1681 cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc
     1741 aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat
     1801 aggtagcgaa ccaaaagtcc acagctggac caacacgcca catatcgcca gaatcaccgc
     1861 cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac
     1921 cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca
     1981 gataaacagt agccagttgc caccaatcag cacggcagag actgccagca taaaaatttt
     2041 ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac
     2101 caccataaag aaaaacgacc agatcacgcg atgcgtcagg atttcatcgg cgggcacgta
     2161 gtaaatcaac ttgaagtacg ctggcgcaat accccaaata aaataagcgg caagagcgag
     2221 taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac
     2281 agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat
     2341 ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg
     2401 caacaggcta ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg
     2461 aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc
     2521 gtggcgggtt aaggtacttc ctacgcacac cagaccggcg gcgacatcca gcgccgacgc
     2581 aatgaccccg ccgtgcaaaa tgctttgcgc ccagttgccc accatcattg gctgattttt
     2641 aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca atgcgcggtt
     2701 aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag
     2761 tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt ttatgcttct
     2821 ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa
     2881 atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc
     2941 ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag
     3001 aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcgccaata
     3061 tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct
     3121 acacccaaac cagcgatctg aacaaagaag cgattgccag ttacgactgg gcggaaaatg
     3181 cgcgtaagga tgaagtaaag tttcagttga gcctggcatt tccactgtgg cgtgggattt
     3241 taggcccgaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca
     3301 atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt
     3361 ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc
     3421 acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc
     3481 tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata
     3541 ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc
     3601 acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg
     3661 gcgcggaatt aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt
     3721 acagcggcta tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtgg
     3781 gggttatgct aaacgatttg ttttgatgaa cggttgagtg gttggcaaat ctggaatcca
     3841 gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa
     3901 aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga
     3961 atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc
     4021 ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg gtcgtcatgc
     4081 ccaccggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta
     4141 ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctgcaagcca
     4201 acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg cgaacagcaa cttgaagtga
     4261 tgacaggctg ccgcaccggg caaattcgtc tgctttatat cgccccggaa cgcctgatgc
     4321 tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc gttgatgaag
     4381 cgcactgtat ctcccaatgg ggccacgatt tccgcccgga atatgccgcg ctcggtcagt
     4441 tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca
     4501 cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt
     4561 ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga
     4621 tgcgctacgt gcaggaacag cgcggtaagt ccggcattat ctactgcaac agccgggcga
     4681 aagtagaaga caccgctgcg cgcctgcaaa gcaagggtat tagcgcggcg gcctatcatg
     4741 ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgacctgc
     4801 aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg
     4861 tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg
     4921 ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc
     4981 tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac
     5041 tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact
     5101 attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga
     5161 aacaatacga tggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc
     5221 agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc
     5281 gcgactatgg tcatgacaaa ctgaaagtct atggcatggg ccgtgataaa agccatgaac
     5341 attgggtgag cgtgatccgc cagctgattc acctcggcct ggtgacgcaa aatattgccc
     5401 agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt
     5461 tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg
     5521 gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg
     5581 atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg
     5641 aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc
     5701 tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat ggcgacgacg
     5761 aagagtagtc agcagcataa aaaagtgcca ggatgaagac tccgtaaacg tttcccccgc
     5821 gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt gtggcgctta
     5881 tgagtcccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag
     5941 aagcgatgat gggcgtgctg ggtattacct gcggcgttat ggtttgggca gggattgcgt
     6001 tgcttggcct gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggttg
     6061 gcggtggcct gtatctctgc tggatgggtt atcagatgct gcgcggcgcg ctgaaaaaag
     6121 aggcggtttc tgcacctgcg ccacaggtcg agctggcgaa aagtgggcgc agtttcctga
     6181 aaggtttact gaccaatctc gctaatccga aagcgattat ctattttggt tcggtattct
     6241 cattgtttgt cggtgataac gttggcacta ccgcgcgctg gggcattttt gcgctgatca
     6301 ttgtcgaaac actggcgtgg tttaccgtcg ttgccagcct gtttgccctg ccgcaaatgc
     6361 gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg
     6421 gatttggcat tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg
     6481 ataaggcgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag
     6541 tgctcccacc agcataaaca acgagccaaa aatcttattc agcgccttca tctgctttgg
     6601 tcctttaatc catagagcaa tccgttgagc aagggtggcg taaccgatca tcacaataat
     6661 atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg gctgttgcgg
     6721 catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt
     6781 cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag
     6841 gtcaattgca ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc
     6901 tgcccacttc aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac
     6961 gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc
     7021 gccgcgataa ccgtggttga gcgaggtggt catagtgttg attgcaccag agcctggcga
     7081 caggcttaaa atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa
     7141 ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac
     7201 gctaaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa
     7261 aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt
     7321 tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg
     7381 tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga
     7441 gctacgtaaa atatgcggaa ctggcctatg acctgttcca tttggggttt gatgtcttaa
     7501 tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc
     7561 atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc
     7621 agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca
     7681 cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt
     7741 ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag
     7801 cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt
     7861 ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg
     7921 ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc
     7981 tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgccaacg cttctcttac
     8041 aagctgaaga ggaacgcgtg gtggataacc gcatgcatga ccgtttttgt gaactccgca
     8101 ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt aattaaaggt gcttaccatg
     8161 agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatc gttgattttt
     8221 tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac
     8281 caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct
     8341 tacgccaaag aaactctgaa gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc
     8401 ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg
     8461 attacctcca atggtgcgcg cgttcacgat ctggatggta atctgatttt tgctcataac
     8521 ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt
     8581 actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc
     8641 ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc ctggattact ggagccggaa
     8701 ggcgtcagca aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag
     8761 gcgattaacg ctcgctgggg cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg
     8821 gaagtgatgg cgggcggcgt gtcgaaaggc catgcgctgg aagcagtggc gaagaaactg
     8881 ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg
     8941 tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agaccttcat
     9001 cccgagctgg aagtgattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa
     9061 ctctatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat
     9121 ggatactcgt taatcaaaga gttttccatt gtggcgctac ttatcatcac cacgattctg
     9181 tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt
     9241 gcggtgctgg tgcgcgttgg cctggcggca ctcgtttttc tgccgtttct gcgtacccgt
     9301 ggcaatagcc tgaaaacggt cggcctgtat atgttggtgg gcgcgatgca gctcggcgtg
     9361 atgtatatgc tgagtttccg cgcttatctc tatctgacgg tttccgaact gctgctgttc
     9421 accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg
     9481 cgctggggct atgcttttag cgccttgctg gcggtgattg gtgccgggat tattcgctat
     9541 gatcaggtca ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact
     9601 tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagcat
     9661 aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg
     9721 ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt
     9781 ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac
     9841 gccggaacgc tgggaattat gaataatatg cacgttccgg cagggctgct ggtaaacctg
     9901 gctatctggc accaacagcc gcactggcca acgtttatta caggcgcgct ggtgatcctg
     9961 gcctcactgt gggtgcatcg taagtgggtc gctccgcgct cttcacaaac ggcagatgat
    10021 cgcaggcgtg attgcgcgct gagcgaataa acgcttccgt aactggctga cgctgctcgc
    10081 catcgcgcac ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca
    10141 ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc
    10201 gcgcggcaac catctgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga
    10261 cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa
    10321 tcaacagcgt ctcgctggcg agatcttccg gtgtaattcg cgttttcgcc gccagtggat
    10381 ggtcaggtgc taacaccaga cgcacttcat agtcgaacat cggcgaataa tgcaggccac
    10441 tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct
    10501 gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga
    10561 aattttctaa cgcgggcgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca
    10621 gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctaatttgc ggcagcacct
    10681 ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac
    10741 gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg
    10801 attgcgtctg atgcaacgtc gccgcagcgg ctgcgagaga gccgcagttc cgcaacgctt
    10861 gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt
    10921 tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa
    10981 atactaaaaa ttccataagg ggcatataat gacaattctt aatcacaccc tcggtttccc
    11041 tcgcgttggc ctgcgtcgcg agctgaaaaa agcgcaagaa agttattggg cggggaactc
    11101 cacgcgtgaa gaactgctga cagtagggcg tgaactgcgt gctcgtcact gggatcaaca
    11161 aaagcaagcg ggtatcgacc tgctgccggt gggcgatttt gcctggtacg atcatgttct
    11221 gaccaccagt ctgctgctgg gtaatgttcc gccacgtcat cagaacaaag acggttcggt
    11281 agatatcgac accctgttcc gtattggtcg tggacgtgcg ccgactggcg aacctgcggc
    11341 ggcagcggaa atgaccaaat ggtttaacac caactatcac tacatggtgc cggaattcgt
    11401 taaaggccaa cagttcaaac tgacctggac gcagctgctg gaggaagtgg acgaggcgct
    11461 ggcgctgggc cacaacgtta agcctgtgct gctggggccg gttacttacc tgtggctggg
    11521 gaaagtgaaa ggtgaacagt ttgaccgcct tagcctgctg aacgacattc tgccggttta
    11581 tcagcaagtg ctggcagaac tggcgaaacg cggcatcgag tgggtacaga ttgatgaacc
    11641 cgcgctggta ctggaactac cacaggcgtg gctggacgca tacaaacccg cttacgacgc
    11701 gctccaggga caggtgaaac tgctgctgac cacctatttt gaaggcgtaa cgccaaatct
    11761 cgacacgatt actgcgctgc ctgttcaggg tctgcatgtt gacctcgtac atggtaaaga
    11821 tgacgttgct gaactgcaca agcgcctgcc ttctgactgg ttgctgtctg cgggtctgat
    11881 caatggtcgt aacgtctggc gcgccgatct taccgagaaa tatgcgcaaa ttaaggacat
    11941 tgtcggcaaa cgtgatttgt gggtggcatc ttcctgctcg ttgctgcaca gccccatcga
    12001 cctgagcgtg gaaacgcgtc ttgatgcaga agtgaaaagc tggtttgcct tcgccctaca
    12061 aaaatgccat gaactggcac tgctgcgcga tgcgctgaac agtggtgaca cggcagctct
    12121 ggcagagtgg agcgccccga ttcaggcacg tcgtcactct acccgcgtac ataatccggc
    12181 ggtagaaaag cgtctggcgg cgatcaccgc ccaggacagc cagcgtgcga atgtctatga
    12241 agtgcgtgcc gaagcccagc gtgcgcgttt taaactgcca gcgtggccga ccaccacgat
    12301 tggttctttc ccgcaaacca cggaaattcg taccctgcgt ctggatttca aaaagggcaa
    12361 tctcgacgcc aataactacc gcacgggcat tgcggaacat atcaggcagg ccattgttga
    12421 acaggaacgt ttgggactgg atgtgctggt acatggcgag gccgagcgta atgacatggt
    12481 ggaatacttt ggcgagcacc tcgacgggtt tgtctttacg caaaacggtt gggtacagag
    12541 ctacggttcc cgctgcgtga agccaccgat tgttattggt gacgttagtc gcccggcacc
    12601 gattaccgtg gagtgggcga agtatgcgca atcgctgacc gacaaaccgg tgaaagggat
    12661 gctgacgggg ccggtgacca tactctgctg gtcgttcccg cgtgaagatg tcagccgtga
    12721 aaccatcgcc aaacaaattg cgctggcgct gcgtgatgaa gtggccgatc tggaagccgc
    12781 tggaattggc atcatccaga ttgatgaacc ggcgctgcgc gaaggtttac cgctgcgtcg
    12841 tagcgactgg gatgcatatc tccagtgggg cgtagaggcc ttccgtatca atgccgctgt
    12901 agcgaaagat gacacacaaa tccacaccca catgtgttat tgcgagttca acgacatcat
    12961 ggattcgatt gcggcgctgg acgcagacgt catcaccatc gaaacctcgc gttccgacat
    13021 ggagttactg gagtcgttcg aagagtttga ttatccgaat gaaatcggtc caggcgtcta
    13081 tgacattcac tcgccaaacg taccgagcgt agaatggatt gagtccctgc tgaagaaagc
    13141 ggcaaaacgc attccggcag agcgactgtg ggttaacccg gattgtggcc tgaaaacgcg
    13201 cggctggcca gaaacccgcg cggcactggc gaacatggtg caggcggcgc agaacttgcg
    13261 tctggggtaa atccaaaccg ggtggtaata ccacccggtc ttttctcatt acagcgactt
    13321 cttcccacca tactgcttaa accattccag catacgctgc cagccatctt tcgcagattc
    13381 ggcatgatag ctcgggcgat aatcagcgtt gaatgcatgc ccggcatccg ggtagacgat
    13441 aatctctgct ttcgcattag cagcccgcag cgcctggcgc atcgtttcaa cgctctcctg
    13501 cggaatgctg ttatcctgac cgccatataa gccgagaacc ggcgcgttaa gatcggttgc
    13561 gatatcaaca ggttgtttcg gtgaattcag cgacttatcg cccgtcagtt tgccgtacca
    13621 cgccactgcg gcttttagct gtggattatg cgcggcatac agccaggtga tacgtccacc
    13681 ccagcagaat ccggtgatca ttaaacgatg aacatcgccg ccgttgcggg aagcccaact
    13741 ggcgacatga tcgagatcgg ccagcacctg cgagtcaggc acttttgcta ccagaccgct
    13801 aagcaacgtg gggatatcgg caaaatcatt cggatcgcct tcgcggaagt aaagttcagg
    13861 tgcgatagcc agatacccct ccagcgccag acggcgacaa atgtcgcgga tatgttcatg
    13921 cacgccaaaa atttcctgca ctacaatgac cactggcagt gggccatcgc tttgctttgg
    13981 tctggcatgg taagcaggca tgttatcccc ttgtgaaggg atagaggtga agcccgccac
    14041 aatcgcgtcg tccggggtct gaacgatggt cgaagcgaga ggcgatgcag caggtgcaaa
    14101 tccagattgt tgtgttgttg ccatggtatt ctccgtaccc ttataaaaat gttgcgcaat
    14161 gttaactata gtcagcatgc aacaaatcac attgcctgaa tcggctcatc ttttatgcag
    14221 tcctgcagaa tgaagagtga tttatgtgat ttgaatcact tttggtgggt aaatttatgc
    14281 aacgcatttg cgtcatggtg atgagtatca cgaaaaaatg ttaaaccctt cggtaaagtg
    14341 tctttttgct tcttctgact aaaccgattc acagaggagt tgtatatgtc caagtctgat
    14401 gtttttcatc tcggcctcac taaaaacgat ttacaagggg ctacgcttgc catcgtccct
    14461 ggcgacccgg atcgtgtgga aaagatcgcc gcgctgatgg ataagccggt taagctggca
    14521 tctcaccgcg aattcactac ctggcgtgca gagctggatg gtaaacctgt tatcgtctgc
    14581 tctaccggta tcggcggccc gtctacctct attgctgttg aagagctggc acagctgggc
    14641 attcgcacct tcctgcgtat cggtacaacg ggcgctattc agccgcatat taatgtgggt
    14701 gatgtcttgg ttaccacggc gtctgtccgt ctggatggcg cgagcctgca cttcgcaccg
    14761 ctggaattcc cggctgtcgc tgatttcgaa tgtacgactg cgctggttga agctgcgaaa
    14821 tctattggcg cgacaactca cgttggcgtg acagcttctt ctgatacctt ctacccaggt
    14881 caggaacgtt acgatactta ctctggtcgc gtagttcgtc actttaaagg ttctatggaa
    14941 gagtggcagg cgatgggcgt aatgaactat gaaatggaat ctgcaactct gctgaccatg
    15001 tgtgcaagcc agggcctgcg tgccggtatg gtggcgggcg ttatcgttaa ccgcacccag
    15061 caagagatcc cgaatgctga gacgatgaaa caaaccgaaa gccaagcggt gaaaatcgtg
    15121 gtggaagcgg cgcgtcgtct gctgtaatcc tcttctcctg tctgaaggcc gacgcgttcg
    15181 gccttttgta tttttgcgta gcgcctcgca ggaaatgcct ttccaactgg acgtttgtac
    15241 agcacaattc tatgttgtgc gggtaagtaa tgaggctgga ggcgttgtgg atttctcaat
    15301 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    15361 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    15421 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gtgaattgct
    15481 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    15541 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    15601 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gctaaccgta tttttgagca
    15661 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    15721 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    15781 agaacgccat accctgaccc acgaaattcg taatctacag caactcaacg cgcaaatggc
    15841 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgat aacaaaaccc aggggaactg
    15901 gggtgaagtg gtactgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    15961 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    16021 gcggttgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    16081 acgctatttc aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    16141 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    16201 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt tactggcgct
    16261 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    16321 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    16381 ccgcaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    16441 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    16501 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    16561 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    16621 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    16681 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    16741 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    16801 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    16861 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    16921 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    16981 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    17041 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    17101 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    17161 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    17221 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    17281 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    17341 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    17401 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    17461 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    17521 tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    17581 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    17641 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    17701 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    17761 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    17821 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    17881 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    17941 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    18001 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    18061 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    18121 gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa
    18181 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    18241 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    18301 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    18361 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    18421 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    18481 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    18541 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    18601 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    18661 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    18721 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    18781 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    18841 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    18901 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    18961 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    19021 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    19081 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    19141 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    19201 cagagctaca cgtcgattct ggctgggtgc caccagatac caacgttgaa gagttcgaat
    19261 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    19321 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    19381 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    19441 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    19501 tcggtattcc tgccctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    19561 tgccagaact gcctgaactg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    19621 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    19681 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    19741 ctgaatgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    19801 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    19861 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    19921 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    19981 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    20041 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    20101 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    20161 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    20221 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    20281 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    20341 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    20401 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    20461 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    20521 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    20581 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    20641 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    20701 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    20761 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca tctatcacct
    20821 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    20881 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    20941 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    21001 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gcatggcgtt
    21061 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    21121 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    21181 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    21241 ctcgccagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    21301 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    21361 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    21421 agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc aaccgcccgt
    21481 cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt gaccagttcg
    21541 caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg
    21601 ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag
    21661 tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca gtggcaagct
    21721 gcgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc gattggtgaa
    21781 tgtggtctcg actttaaccg caacttttcg acgccggaag agcaggaacg cgcttttgtt
    21841 gcccagctac gcattgccgc agatttaaac atgccggtat ttatgcactg tcgcgatgcc
    21901 cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg tgcggttctt
    21961 cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc
    22021 ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga attgttgccg
    22081 ttgattccgg cggagaaatt gctgatcgaa actgatgcgc cgtatctgct cccgcgcgat
    22141 ctcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcccca tattttgcaa
    22201 cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac ggatgccaat
    22261 gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt cttcacactg
    22321 tgcttaatct ctttattaat aagattaagc aatagcatgg agcgagcctc accatcgggt
    22381 tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg taataatcac cttatcaccc
    22441 ggataagggg ttgccggatc gacaatgtct ttcggtttat ataccgatag ctgatgaatc
    22501 accgccgatg ggactatcgc tggcgacgcg ccaaagcgca cgaagtggct gacaccgcgg
    22561 gtcgcgttga tagtcgtggt atgaatcact tctggatcaa attcgacaaa caggtagttg
    22621 ggaaacaatg gctcactgac tgcagtacgt tttccacgca cgattttttc cagggtgatc
    22681 atcggtgcca ggcaattcac agcctgtctt tcgaggtgtt cctgggcacg ttgaagttgc
    22741 ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg tttaatcggg
    22801 gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt gcgttatacc
    22861 gtcagagttc acgctaattt aacaaattta cagcatcgca aagatgaacg ccgtataatg
    22921 ggcgtagatt aagaggctac aatggacgcc atgaaatata acgatttacg cgacttcttg
    22981 acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg
    23041 gaaatcactg aaattgctga ccgcactttg cgtgccggtg ggcctgcgct gttgttcgaa
    23101 aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg
    23161 gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa attattggcg
    23221 tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa actgccgcag
    23281 tttaagcaag tattgaacat gccgacaaag cggctgcgtg gtgcgccctg ccaacaaaaa
    23341 atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg ctggccggaa
    23401 gatgccgcgc cgctgattac ctgggggctg acagtgacgc gcggcccgca taaagagcgg
    23461 cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat tatgcgctgg
    23521 ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca tccgggcgaa
    23581 cgtttcccgg tttctgtggc gctgggtgcc gatcccgcca cgattctcgg tgcagtcacc
    23641 cccgttccgg atacactttc agagtatgcg tttgccggat tgctgcgcgg taccaagacc
    23701 gaagtggtga agtgtatctc caatgacctt gaagtgcccg ccagtgcgga gattgtgctg
    23761 gaagggtata tcgaacaagg tgaaacggcg ccggaagggc cgtatggcga ccacaccggt
    23821 tactataacg aagtcgatag tttcccggta tttaccgtga cgcatattac ccagcgtgaa
    23881 gatgcgattt accactccac ctatactggt cgtccgccgg atgaacccgc ggtgctgggt
    23941 gtcgcactga acgaagtgtt tgtgccgatt ctgcaaaaac agttcccgga aattgtcgat
    24001 ttttacctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat caaaaaacag
    24061 tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg
    24121 tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg
    24181 atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt agaaaatacg
    24241 cctattgatt atctggattt tgcctcgcct gtctccgggc tgggttcaaa aatggggctg
    24301 gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa
    24361 gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat ttttaacaac
    24421 ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca gagaaagcgc
    24481 atgacaacct taagctgtaa agtgacctcg gtagaagcta tcacggatac cgtatatcgt
    24541 gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt gatggtagtg
    24601 atggatgagc gcgacaaacg tccgttctca atggcctcga cgccggatga aaaagggttt
    24661 atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt catggaccgc
    24721 atcctcaaag atcatcaaat cgtggtcgat attccccacg gagaagcgtg gctgcgcgat
    24781 gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctctta tgcccgctcg
    24841 attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta ctggggcggg
    24901 cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt gaagcatcct
    24961 ggtctgcaag tggtgccggt ggttgaacaa ccggaagcgg gctggcgtgg gcgtactggc
    25021 accgtgttaa cggcggtatt gcaggatcac ggtacgctgg cagagcatga tatctatatt
    25081 gccggacgtt ttgagatggc gaaaattgcc cgcgatctgt tttgcagtga gcgtaatgcg
    25141 cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa aaaacccgcc
    25201 cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag atctatgtcg
    25261 catccggcat tcggcacacg atgcctgatg cgacgctggc gcgtcttatc aggcctacat
    25321 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
    25381 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
    25441 tggacgtctt tgcgctccat cagatttagc agcgtggtgc tgatacgcgc accggaacag
    25501 cccagcggat gacccagcgc gatcgcgcca ccgttgaggt tgatcttctc gtcaatctgc
    25561 tccattagcc ccagatcttt aatacatggc aggatctgcg cggcaaaggc ttcattcatt
    25621 tcaaacacgc cgatatcact gacagaaagc cccgcttttt tcagcgccag cttcgaggcc
    25681 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcacg
    25741 cgggcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
    25801 gctgcgccat cggaaagcgc agaagatgta cccgccgtta ccgtaccgct tactggatca
    25861 aacgccggac gcagcgtggc tagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    25921 taattaaact gcttcaggac gccgtcggca tcgtgaccac cggtcgggat aatttcattt
    25981 ttaaatgcac ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
    26041 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
    26101 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcaac gccgtgactc
    26161 atcggcacat ggcccatatg ctccacgccg ccaaccagac atgcctgcgc atcgccggtc
    26221 ataatcatcc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattgacg
    26281 gtaaccgccg ggacagagtg tggtacttct gccagcagcg ccgcgttacg ggcgatgtta
    26341 aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
    26401 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttctgca
    26461 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    26521 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    26581 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
    26641 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
    26701 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    26761 accagcgcca tatccgcttc cgccggagtg gcgataatgc tttcttccag acagcgcacc
    26821 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
    26881 ttcggctggc tcacttctgc cagcaggtcg tcaacgacgg cgtcttcttc tttcttcggc
    26941 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    27001 ttggcatcaa acagcgcgtc gatggtatcg cgataatctt tctgcatccg ctgcgggaag
    27061 cctgctgcca tgacagcctg ggcatgatgc gcggtgtcaa tgcccacgac atccagcaga
    27121 tatgccggac ccatcggcca gccaaactgt ttttccatta ctttgtcgat cttgcggaaa
    27181 tccgcgccgt cgcgcagcaa caggctgaaa ccggcgaaat acgggaacag cacgcggttg
    27241 acgaagaagc cggggcagtc gttaaccaca atcggcgttt tgcccatctt gctcgcccag
    27301 gcgacgactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaattat ttctaccaac
    27361 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttctgg acgttccagc
    27421 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtattcggg
    27481 cgtactttct gctcggtttc tgccagtacg gcttttttca cttttgggtt ttcgacaacc
    27541 gcttctacca caacatccac gcggtcaaac ccggcgtagt cgagcgttgg gtggattgtg
    27601 gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg cttgttcagc
    27661 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    27721 accggcacgc ctttccacgc agactggtaa gcgatgccgc cgcccataat gcctgcaccc
    27781 agcaccgcgg cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
    27841 acatattgat cgttaaggaa aatgccgacc agtgcgcggg cttcgttggt atgcgccagc
    27901 gggacaaaac ttttgttttc caggtttaag gcttcttcac gaccaaaacg ggccgcagct
    27961 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgctgt ttgtgcgacc
    28021 atccctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagtggttcc
    28081 agcttcggct gacgttttgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaacc
    28141 gcctttgcgc cttcaaccag tttttctgct ttgactacgc catccaccag accgattttc
    28201 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc actgtcagcg
    28261 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    28321 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    28381 tcgcagccac cgcccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
    28441 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    28501 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    28561 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    28621 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    28681 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    28741 gtctcctgaa tccgcaaggt gatctggtcg taccagatgc atcgaagtgt gtattttgtg
    28801 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
    28861 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    28921 aaaaaatgga atcactggcc tcactctata aaaatcatat agctacctta caggaacgga
    28981 ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc ggcgaactgt
    29041 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    29101 gggtgccggt aactcaggtg ccaaactgct ggctgctggt ggatggcgtg aacaagccga
    29161 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctctt
    29221 tctggactga agacgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
    29281 tgcctgctgc gcgcgggaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    29341 tgggtattga ggccagcaat atcaacccga aaggggtgat cgactacctg cattactacc
    29401 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    29461 acggtcatcg cgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaaca
    29521 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
    29581 cgctcaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
    29641 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
    29701 cccgtacctg gtcggcaaaa agtgacaacg actacgcaca gctggtgaaa gacgtaaatg
    29761 atgaacaact ggcgctgatc gctaccatga aagcaggcgt cagctatgtg gattaccaca
    29821 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    29881 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
    29941 atccgctggg cctgcaggtg catgacgtag ccggttttat gcaggatgat agcggtacgc
    30001 acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc cagccgggca
    30061 tggtgttaac catcgaaccg ggtatctact tcattgaatc gctgctggca ccgtggcgtg
    30121 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    30181 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
    30241 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    30301 agagatcaaa aaaagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
    30361 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    30421 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
    30481 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
    30541 taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    30601 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
    30661 attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
    30721 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    30781 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    30841 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    30901 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
    30961 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    31021 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
    31081 cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttctgg
    31141 accgtgctgg gtagtgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    31201 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
    31261 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
    31321 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
    31381 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    31441 attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    31501 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    31561 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagcccgact
    31621 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
    31681 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    31741 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
    31801 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
    31861 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    31921 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
    31981 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    32041 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    32101 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    32161 acgggcgtgg atgacttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
    32221 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    32281 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt gctctttacc
    32341 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    32401 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    32461 tccaggcgga tgtcgccaat gtgcaccgca ttgaagaacc acagtgggaa aactatgacc
    32521 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    32581 tcaaaaagca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
    32641 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    32701 tgaactcgca atggcgtccc gatcgctgtg ccgtcattgc cggggcgctg cgttacccac
    32761 gttatcgctg gtacgaccgt tttatgatca agctgattat gaagatgtca ggcggtgaaa
    32821 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    32881 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaa
//
LOCUS       DAEVZA010000040        32414 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEVZA010000040 DAEVZA010000000
VERSION     DAEVZA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.040"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(90..4652)
                     /gene="sslE"
                     /locus_tag="LAF92_001995"
     CDS             complement(90..4652)
                     /gene="sslE"
                     /locus_tag="LAF92_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HBI9791011.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRV
                     TGDITCNDESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDA
                     QELAGSDNKKSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFK
                     KLVNEEVENNAATDKAPSTHTSPVVPATTPGTKPDLNASFVSANAEQFYQYQPTEIIL
                     SEGRLVDSQGDGVVGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKS
                     TIALTELGDEVRGANIDQLIHRYSKAGQNHTRVVPDEVRKVFAEYPNVINEIINLSLS
                     NGATLGEGEQVVNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQ
                     IQGVINKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARND
                     KNYWLAFGEKRAWDKNELAYITEAPSIVRPENVTRETATFNLPFISLGQVGDGKLMVI
                     GNPHYNSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKS
                     SMTVGTNLDTVYFKKHGQVLGNSAPFAFHKDFTGITVKPMTSYGNLNPDEVPLLILNG
                     FEYVTQWGSDPYSIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESA
                     SGFVRLLDAAGLSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTID
                     DTTKEVIWKYQQENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKQ
                     RILDAFPGLEVCKDSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLDLGADTAKAMLQ
                     AADLGTNIQRLYQHELYFRTNGRQGERLNSVDLERLYQNMSVWLWNETKYRYEEGKED
                     ELGFKTFTEFLNCYTNNAYVGTQCSAELKKSLIDNKMIYGEESSKAGMMNPSYPLNYM
                     EKPLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTG
                     LWAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVK
                     FKVPYGGLIYIKGDSKDNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFV
                     YTAPKKNLNASNYTGGLKQFANDLDTFASSMNDFYGRNEEDGKHRMFTYKNLTGHKHR
                     FANDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGA
                     TEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLK
                     EWAEKNFDIKQWYPEGNLPKFYSEREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYCA
                     ESNGNAADTLMLCASWVAQTDLSAFFKKWNPGANAYQLPGATEMSFEGGVSQSAYNTL
                     ASLGLPKPQQGPETINQVTEHKMSAE"
     gene            complement(5138..6820)
                     /gene="glcA"
                     /locus_tag="LAF92_001996"
     CDS             complement(5138..6820)
                     /gene="glcA"
                     /locus_tag="LAF92_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HBI9791012.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(7176..9347)
                     /gene="glcB"
                     /locus_tag="LAF92_001997"
     CDS             complement(7176..9347)
                     /gene="glcB"
                     /locus_tag="LAF92_001997"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBI9791013.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(9369..9773)
                     /locus_tag="LAF92_001998"
     CDS             complement(9369..9773)
                     /locus_tag="LAF92_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HBI9791014.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(9778..11001)
                     /gene="glcF"
                     /locus_tag="LAF92_001999"
     CDS             complement(9778..11001)
                     /gene="glcF"
                     /locus_tag="LAF92_001999"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HBI9791015.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLERARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(11012..12064)
                     /gene="glcE"
                     /locus_tag="LAF92_002000"
     CDS             complement(11012..12064)
                     /gene="glcE"
                     /locus_tag="LAF92_002000"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBI9791016.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGEGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(12064..13563)
                     /gene="glcD"
                     /locus_tag="LAF92_002001"
     CDS             complement(12064..13563)
                     /gene="glcD"
                     /locus_tag="LAF92_002001"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HBI9791017.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            13814..14578
                     /gene="glcC"
                     /locus_tag="LAF92_002002"
     CDS             13814..14578
                     /gene="glcC"
                     /locus_tag="LAF92_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBI9791018.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(14585..15727)
                     /locus_tag="LAF92_002003"
     CDS             complement(14585..15727)
                     /locus_tag="LAF92_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI9791019.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            16089..17819
                     /locus_tag="LAF92_002004"
     CDS             16089..17819
                     /locus_tag="LAF92_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBI9791020.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTVQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
     gene            17816..18730
                     /locus_tag="LAF92_002005"
     CDS             17816..18730
                     /locus_tag="LAF92_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBI9791021.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            18762..19010
                     /locus_tag="LAF92_002006"
     CDS             18762..19010
                     /locus_tag="LAF92_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI9791022.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            19010..20182
                     /locus_tag="LAF92_002007"
     CDS             19010..20182
                     /locus_tag="LAF92_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI9791023.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(20218..21297)
                     /gene="lptG"
                     /locus_tag="LAF92_002008"
     CDS             complement(20218..21297)
                     /gene="lptG"
                     /locus_tag="LAF92_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBI9791024.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(21294..22364)
                     /gene="lptF"
                     /locus_tag="LAF92_002009"
     CDS             complement(21294..22364)
                     /gene="lptF"
                     /locus_tag="LAF92_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBI9791025.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(22395..22955)
                     /locus_tag="LAF92_002010"
     CDS             complement(22395..22955)
                     /locus_tag="LAF92_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBI9791026.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(22967..23791)
                     /locus_tag="LAF92_002011"
     CDS             complement(22967..23791)
                     /locus_tag="LAF92_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791027.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            complement(23791..25140)
                     /locus_tag="LAF92_002012"
     CDS             complement(23791..25140)
                     /locus_tag="LAF92_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBI9791028.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(25186..25944)
                     /locus_tag="LAF92_002013"
     CDS             complement(25186..25944)
                     /locus_tag="LAF92_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791029.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(25976..26689)
                     /locus_tag="LAF92_002014"
     CDS             complement(25976..26689)
                     /locus_tag="LAF92_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBI9791030.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            26863..27555
                     /locus_tag="LAF92_002015"
     CDS             26863..27555
                     /locus_tag="LAF92_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBI9791031.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANRPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(27604..29103)
                     /gene="pitB"
                     /locus_tag="LAF92_002016"
     CDS             complement(27604..29103)
                     /gene="pitB"
                     /locus_tag="LAF92_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBI9791032.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(29395..31254)
                     /gene="gss"
                     /locus_tag="LAF92_002017"
     CDS             complement(29395..31254)
                     /gene="gss"
                     /locus_tag="LAF92_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBI9791033.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            31459..32325
                     /gene="yghU"
                     /locus_tag="LAF92_002018"
     CDS             31459..32325
                     /gene="yghU"
                     /locus_tag="LAF92_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBI9791034.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT         7869 a         8666 c         7987 g         7892 t
ORIGIN      
        1 gataagcttg cgcatcgggc atatgtgttg acccggtgcg ccttatttca tgccggatgc
       61 ggcgcgagcg ccttatccgg catacaggct tactcggctg acatcttatg ctcggtaacc
      121 tgattaatgg tttccggccc ttgctgcggt ttcggcagac caagcgacgc cagcgtgttg
      181 tatgccgact ggctcacacc gccctcgaag ctcatctccg tcgctcccgg caactggtaa
      241 gcattcgcgc ccggattcca tttcttaaag aatgcggaaa ggtccgtctg agcgacccat
      301 gaggcacaca gcatcagcgt gtccgcagcg ttaccgttgg attcagcaca gtaattcttg
      361 ccaccaaagg tcttgtcacc aacgtcatcg ccgcgtgctt tacgatgcat caactggaac
      421 aggttccagc ctttcatccc ttcacgctcg ctgtagaact taggcaggtt accttctgga
      481 taccactgtt tgatatcaaa gtttttctct gcccattcct tcagctgcgc gtacatcagc
      541 agacggtcac ccgcaccgcc acgtgcccat gcctggttgt tgctctcctc cagatattcc
      601 ggtgcgacgg taatatcgtc agcaacacgg ttcatcttgc cgagatagcg atcctgcatg
      661 tacagcgcca gcacgttgtt cgccacttcg gtcgcgcctg gaacagtcag cggcgtttct
      721 gcggcgttgt gccctacttc gtgccagatc agccagtcgt tcagcggcgt cgtcggcagc
      781 gtggtgctgt tcgtcgagaa gctgctgttc attaccggat aacccgaatg cgcatcaccg
      841 atggagatct gcacatcgtt ggcgaaacga tgcttgtggc ccgtcaagtt tttataggta
      901 aacatccggt gcttaccgtc ttcctcatta cgaccgtaga aatcattcat cgagctggca
      961 aaggtatcca gatcgttagc gaattgcttc agtccgccag tgtaattgct ggcattcagg
     1021 ttcttcttcg gtgcggtata aacgaaagcg tctgattcca gctcacccag cggagccggt
     1081 gagttcagat cgtttttcca tgcgccgtct ttatagaacg gtgcttttac cacaccagta
     1141 aaggtgaagc tggcagattc attgtctttg ctgtcgcctt tgatataaat cagaccaccg
     1201 taaggcacct tgaatttcac cgtaccacta gcgtccagag agtacgtttt ggttactctt
     1261 ggcggacggt tcagcgcgac ttcatgcttc tctcttccgg tcaggtcgtc agccagcgcc
     1321 acggtgacgg tcacaggaac gttcgcattg gacttaatgg tgacctcttt ctgagccggt
     1381 gcccacaggc cagttgactg catgttacct gcaaaccatt tggtcggatt cgagtacagg
     1441 ctgatggttt cagtaacgtt ctgtccctct tccgatactg ctcccggata cttctcaaca
     1501 tcaactttga tgttcagatc ccaccaggaa cggcccagca tcaggcgtgt cagcggtttt
     1561 tccatatagt tgagcgggta gctcgggttc atcatgcccg ctttgctgct ttcttcaccg
     1621 taaatcatct tgttatcgat cagcgatttt ttcagctcag cggaacactg cgtgccaacg
     1681 tatgcattgt tggtgtagca gttcagaaac tcagtgaacg ttttaaagcc cagctcgtct
     1741 tctttaccct cttcataacg atatttcgtc tcgttccaca gccagacgga catgttttgg
     1801 tataaacgtt ccagatcgac gctgttgaga cgctcaccct ggcggccatt ggtacggaaa
     1861 taaagctcat gctgatacag gcgctgaata ttggtgccta aatccgcagc ctgcagcatc
     1921 gctttcgcag tgtcagctcc aagatccagc tgcgtatact gcggaacata catgccaccg
     1981 gttaccggca cacccgtgcc tgggcggtat tccagacagt tgacctcata gtgatagtca
     2041 gaatccttac acacttccag ccctgggaac gcgtccagaa ttctctgttt tgccgcagcc
     2101 agtgactcag gcgttttgtg gtcggcttca tcaataaagg cgaattgggt tacctgttta
     2161 ccctcaactt cttcctgcca gctggcaact tccagcttcg gtttgtcatc aggtttgttt
     2221 tcttgctgat atttccagat aacttctttc gtggtgtcat caatggtata cggtggttta
     2281 ccatcgacag ccggataacg ctcatagacc caaattggcg ttgaacgtcg ttgacgaaca
     2341 cggtcagggt agccttgcgg atcgttattt actaccgact tgttaagcgc catcgacaaa
     2401 cctgcggcat caagcagacg tacaaagcca gatgcgctct cttccttaag attgctcatc
     2461 acgttttcca tgatcagcac cgatccacct ttgttcatat aggcgatcaa atcggtcaca
     2521 tcctgctggg tcagcttcgg tttgctggta tctgcgcgca gaggaatgga gtaaggatcg
     2581 ctaccccatt gtgtgacgta ttcaaagcca ttgaggatca acagaggaac ttcatctgga
     2641 ttcagattgc catagctggt cattggtttg accgtgatgc cagtgaaatc cttgtggaac
     2701 gcaaacggtg cgctatttcc cagcacctga ccatgttttt tgaaatatac cgtgtccagg
     2761 ttagtaccca cggtcatact ggattttgca tccggcaacc agcgatcatt tgacagatag
     2821 cgcagcacgt tctccatgaa gttcttcatg tcatccgggt cgctgttgag cgtacactgt
     2881 ccgtctttat taacgccccc gttccagctg tagccgttcg ggcaacgcag gatgctgttg
     2941 tagtgtgggt taccgataac catcagcttg ccatcgccca cttgccccag cgagataaac
     3001 ggcaggttga aggtggcggt ttcgcgtgtc acgttctctg gtcgcacaat ggaaggcgct
     3061 tcagtaatgt acgccagctc atttttatcc caggcgcgtt tttcgccaaa cgccagccag
     3121 tagtttttat cattacgcgc catcagaatc gggaaggccg cgttggagat attcaccacc
     3181 gcctgaccgc gcgcattacc cgtgctgcca tagaagttgg tagagtcatg gaatacatgg
     3241 aacttactga cagatttgta gttcgtatcc acgccccaca gcttgttgat aacgccctga
     3301 atctggccgt cattaacatt gcgcgttgtc agcgagaacc agcgagcctc gttacaacca
     3361 tcggttttcg cacaaatcgc ggtatcgatc tctttggcct gacccgtctt aaactgctcg
     3421 ataaattcgt taggcagatt aacgacttgc tcaccttccc ccagcgtcgc accattggat
     3481 aacgagagat tgataatctc gttaatcacg ttgggatatt cagcaaaaac cttgcgcact
     3541 tcatccggaa ctacacgcgt gtgattttgt ccagccttcg aatagcggtg aatcaactga
     3601 tcgatatttg ccccgcgaac ttcatcaccc agttcagtca atgcaatagt cgacttgtta
     3661 ccacgcacag aaccaagctc aaaagtgtcg atgccaaagg agatggtttc cccccaacta
     3721 aaggaaaatt ccccgttttc tccggttaca ccacggccgg aattggtgta atagttgaca
     3781 ccaacaacac catccccctg actatcgacc agtcgacctt cagagagaat gatttcagtg
     3841 ggttgatact gataaaactg ttccgcgtta gccgacacga aggaagcgtt tagatccggt
     3901 tttgttcccg gagtggtggc gggcacgacc ggtgaagtat gagtggatgg cgctttatcg
     3961 gtcgcggcat tgttttccac ctcttcattg accagctttt tgaattcttc cggtgccaga
     4021 tcgatttgct tatacagcga gtcgaaacgt ttactctcga tcaccgagga gaactccagg
     4081 cacacctgtt ctgtattcgc cgggcaactg ttcatggagg tgaccagcga gagcgcattg
     4141 cttttcttgt tgtcggaacc cgccagttct tgcgcgtcct caagactaaa cgacactttt
     4201 tcaaccgcac gcaggctacg cgcagcttct gactgggtgt cgaaggtagc aattgtcgtg
     4261 ttgttccctg ccacacaggt gactttgtcg cctggtgtaa aggtaaagcc atcgctggat
     4321 tcatcattac aggtgatatc accagttacc cgcaggcttc cgcccagagt cagataaccc
     4381 gttttcgtag gaacaggttc tggctccggt tctggtgttg gctcaggatc aggtgtcggt
     4441 tccggcgtag gttctgggtc cggcgttggc tcaggcgtcg gctccgggtt tggtgttgga
     4501 tcaggtttca cttccggcaa agaccctgtt ccagaatcta caggcggcgt atcggaggaa
     4561 gatccggagc caccgccatc acaaccggct aacagggttg cactcaaaat agccgctaaa
     4621 agcgatttct tatatttaaa tttcttattc attaataacg caagtgacaa aacaatgtta
     4681 aaaaattcgt aactgggaga aatagtttta tgctttatct tctttaataa tttcctccat
     4741 tcttaagaaa acgacatcat tgatagaaaa caggtgaaat ttataagaat aacccctatt
     4801 tgatgtctgg ctggctattt ttacgaaatt tcaataaaca aaaaattaac aacatttcac
     4861 aacgcaacta tataaattcg ttggtagttt taacgattaa gattgcaaaa gcaacagata
     4921 gaacggtgtt tgctcatcaa gcgaacataa ttaatacaga cttgttttgc gtgataaaaa
     4981 aaatagatgt tctcacgctc ttaattattt agcaggttat ctgaacacaa aacattattg
     5041 ccgcatggat aataaagcga gaaataattt tcaattaata agacagaata atgtaaatga
     5101 agccggatga tattaacgat catccggcat tattgattta cgagaccaac attccagtaa
     5161 acacatacgc ctgcaacaac gtgataacgc cgataacgct ggcaaaaatc agactgtgct
     5221 tcacggtgta gcggaatagt tcagattctc ggcccaccat gcccgtcgcg gcgcaggcca
     5281 cggcgataga ttgcggggag atcatcttgc cagttacgcc gccgctggta tttgctgcca
     5341 ccagcagagt gtcagagacg ttgatttgct gcgccgtggt cgattgcagt gaaccaaaca
     5401 gggcgtttga agaagtgtcc gagccggtaa ggaatacccc cagccagccg aggaacggtg
     5461 agaaaaacgg gaacatcacg cctgtacctg ccagtaccag cgccaacgtg gtggacatgc
     5521 cggaatagtt ggtgacgaag gcgaacgcca gcaccatgcc aatcgacaat atcggccact
     5581 tcagactaat tagcgtttcg gcaaagacgc caatcccttt cttgatgccc acgccgagga
     5641 tgaagataga gataatcgcc gcaataaaaa tagcggtgcc gccagccgag agagggtcga
     5701 atttaaacac cgcatccatt ggcgttggtt gggcgacaat gggtgccgct ttcaacactt
     5761 gttgatgcaa atgagggatc tggaaattaa tcaccagtga ataaaacgcg ccgcccggag
     5821 caaataacgc tttaaacggc ttcatggtcc agatggtgac cagcaccgtt aagattaaaa
     5881 acggtgacca cgcacgaatg atttgcccca gactatattc tgaaggcacg ggaccgccag
     5941 aagatggctt atttaccacc atcgcacctg cggattgccc catgctgatt gccgtttcgg
     6001 tatttttcgg ccgccagact ttaaggaata aagcgagtga gacgatactc accagcgccg
     6061 aagtaatatc cggcagttcc ggaccaatat agttagaggt aaagaactga gtgacagcga
     6121 agcttccccc agcaaccagc gccgctggcc acgtctcttt cacccctttc cagccgtcca
     6181 tcattgctac cagccagaac ggcacaagaa ccgacaggaa cggtaactga cgtcccgcca
     6241 ttgcgccaat gtggaacgga tcgattcccg ttacctgacc ggcgaccaga atcggcacgc
     6301 ccaacgcacc aaacgccact ggcgcagtgt tggcaatcag acacagcccc gccgcgtata
     6361 acggtttgaa gcccaggccc accagcagcg caccggtaat cgccaccggc gcaccaaagc
     6421 cagccgctcc ttccagcaac gcaccaaagg agaaaccaat cagtaacacc tgcaaacgct
     6481 gatcgtcggt gatggagata accgagctgc ggataatatc gaactgaccg ctggcaacgg
     6541 ttaatttata gaggaacacc gccgcgacaa taatccacgc aattggccat aatccataaa
     6601 taaagccata gcctgcagca gcaaatgcca tatcaatcgg cattttaaag gcgaatatcg
     6661 caatcaggat agataagata agggttattg ctccagcgac atgtcctttc agacgtaata
     6721 ccgcgagtgc aacgaagaag aatattatcg ggatcagggc gaccagagcg gatagcccca
     6781 gtcctcccat cggcatatac atttgggtcc aggtaaccat attgttcagt ctcttattat
     6841 ctttatatgc ttgatatact taaggttgta ataagcaaaa gaggactgaa ctgtaaaata
     6901 taggcgttat actttacagc aacagtacgc cgctaacgca attgctacct ctggcataac
     6961 aagtatatcg ggtaagggtt tctgttccgc acacgcaggc gcagagtatc gttaagatgt
     7021 ccatattgtt gttttaggcc cgctagtaat gcgctacggg tatttaatat tgttaaaccc
     7081 tgataatcgc tccggttatt tccgggataa atgtactatc gcagttacta tcatagcccc
     7141 gacaataaaa cttgccgggg ctttttttga cgctattaat gactttcttt ttcgcgtaaa
     7201 cgccaggcgt gtaataacgg ttcggtatag ccgtttggct gtttcacgcc gaggaagatt
     7261 aaatcgctgg cagctttaaa agcacacgag ttagcgaaat tccccgccat cggacgataa
     7321 gccggatcgc cagcgttttg ctgatcaacc actttcgcca tattctccag cgacgcctgc
     7381 acctgttctt tggtcagaat accgtgacgt aaccagttgg cgatatgctg gctggagata
     7441 cgcagcgttg cgcggtcttc catcagcgcc acattgtgaa tatccggcac ttttgaacaa
     7501 ccaatcccct gctccaccca gcgcaccacg taccccagaa tcccctgcac gttgttatcc
     7561 agctcttgtt ggatctcttc caccgaccag ttagcgtttt cagccaccgg aatagtcagc
     7621 agatcgtcca gcagcggttc aaattcagca ttgaactcgg tctgggcaat gttggcttgc
     7681 acgctctgta cgttggtttg gtggtagtgc agcgcatgga gcgtagcagc ggttggtgac
     7741 ggaacccagg ctgtgtttgc cccggcacgc agttggtcgc ccttctggct gtacatgtct
     7801 gccatcaggt ccggcattgc ccacatgcct ttaccaattt gcgctttacc gcgcagccca
     7861 cagaacagac cggaaagcac gttattacgc tcgtaggctt tgatccacgg cgtcgatttc
     7921 atctggtttt tacgcagcat cgggccagct tccatcaccg aatgcatttc atcgccggta
     7981 cggtcgagga aaccggtatt gatgaacgcc acgcgattgc gcgcctgagc gatacagcta
     8041 cgcaagttca gcgaggtccg acgttcttca tccataatgc ccattttcag ggtattcggt
     8101 gccataccga gcattgtctc aatgcgggta aacagtttgt tggcgaacgc cacttcctgc
     8161 ggaccgtgca ttttcggttt cacaatatag acgctgccag tgcgcgagtt tttctgcact
     8221 tttaaatcat agagggcgat cgcgccagtc atgacgccat caagaatgcc ttccgggatt
     8281 tcattgcctt cgctgtccca aatcacagga atggtcatca aatgacccac gttgcggata
     8341 aacagcagcg agcgtccgtg cagagaaatt tcagagccat cggcggcggt gtaatgacga
     8401 tcgtcattca gtttacgcac gatttgtcga ccgtttttct ccattttctc ttgcagagtc
     8461 ccctgcatca ggcccagcag attgcggtac agcaggattt tatcttccgc atcaaccgcc
     8521 gcgaccgaat cttcgcagtc gagaatggta ctgatagccg cttcgacgat aacatcgttg
     8581 atgtgcgccg gatcgtcttt gccaatccgc ccgttggcat caatttgcag ttcaatatgc
     8641 aggccgttat ttttcagcaa aatgcaggtc agcgcagcgg catcgccacg gtaaccgaca
     8701 aactgcgccg gggtacgtaa cgtggtttct ttaccatttt tcaactggat gcgtaattgt
     8761 ttatcgacca ccttaaacgc caccacatcc tgatagctgc cattttccag cggtagagat
     8821 tcatcgagga aacgccgaac ccaggcgata acctgctcac cgcgttgcgg atcgtagccg
     8881 ctgaccatcg ccccttcctg cgggatgatg tcgctgccgt ataacgcatc gtacagtgag
     8941 ccccagcgag cgttcgccgc gttcagcgcg tagcgggcgt tcattgccgg aaccaccagc
     9001 tgtggccccg cctgactggt gatttcgctg tcaatgcccg tggtttccac cgtcacgcgc
     9061 tccggttgcg gcaccaggta gcccagttca cgcaggaaag atttataggc cgctttatct
     9121 tttaccggcc ccggattgct gcgatgccac tcatcaagcg ccgcctgaat gcgatcgcgt
     9181 tctgccagca actgacgatt ttctggtgcc agatcgtgaa cgatttcatc aaaattgcgc
     9241 cagaacgccg cagcgtccag ccctgttccc ggtaaaactt cttcatccac aaaacgttta
     9301 aaattggcgt caatgcgtaa acggccctgg gttatggttt gactcattgt ttatctcctc
     9361 gttttcgctt atttcgccaa caccgctgct gccgctttcg cgacctgcgc atcctgtgct
     9421 ccggttaaac cagaaacgcc cacggcacca ataatttgcc catcaacaac aaccggtacg
     9481 ccaccttcca gcgacgtcag taacggcgca gtcacgaacg cggtacgtcc gttgttcacc
     9541 atctcttcat agcccttagt ttcacgacgc cccagcgcgg cggtacgcgc tttctcctgg
     9601 gagatgtaag ccgcaatcgg cgcgcaatcg tccatgcgac ttaacgccag cagatgaccg
     9661 ccgtcatcgg caacagcaat ggaaacagac cagttatttt tctgcgcctc ttcctgacct
     9721 gcggcaataa ttgcactcgc catttgctgg ctaagaatga ctttagtttt cattttttta
     9781 ttccttttca agggcttgtt ctacaatttc aatccagtga cgcacagagg tacgaccggc
     9841 gctcgccaga tgcgtctggc aaccaatgtt ggcggtgacg atcatttccg gtttgccgct
     9901 ttccagcgca ttcattttgt tatcccgcag ctggcgtgcc agatcgggat gcgttaacgc
     9961 atatgttccc gctgaaccgc agcacagatg gctgtcggga acgtccgtta aggtaaatcc
    10021 aagacgaagc aacacttttt ccacttcgcc gttcagcttt tgcgcatgtt gtagggtaca
    10081 cgggcagtgg aaggccagtt ttttatcgcc gcgaactgcc agtttttcca gcggttcctc
    10141 gcgcagaagc tcgactaaat cgaccgccag ttcactgact tgacgcgctt tatcggcata
    10201 taacgcatcg tttttcagca tctgcccgta ctctttgaca aacgcgccgc aaccgctggc
    10261 ggtttgcaaa attgcctcgg cacctgcttc aatcgcgggc caccaggcat caatattatt
    10321 gcgcgcccgt tccagccctt tctcctgcgc attaagatga tagtccaccg cgccacaaca
    10381 gcctgcttcg ttagctgaca tgacgctgat ccccagacga tccagcactc gcgcagttgc
    10441 cgcgttggtg ttgggcgaaa gcgtaggctg ggcgcagcct tccagcatta aaacccgacg
    10501 cttatggcgc agcggcggac gcggtttagc tttcaccgtt tcagcaggca gttttgctct
    10561 gacctgttcc ggtaaaaacg gtcgcagcac cagccctacc tgcgtcagcg cacggaagac
    10621 cgccggacgc ggcactacct ggcgcaatcc ttcgcgcagc attcgctccg gcagtgggcg
    10681 tttcactttc tgctcgacaa tatcacgccc gatatccagc aaattgtgat agcgcacacc
    10741 agaaggacag gtggtttcac aattgcggca agtgaggcag cgatcgagat gctcctgtgt
    10801 tttaagcgtg acttcgttgc cttccagcac ctgtttaatc agatagatgc gcccgcgcgg
    10861 cccgtccagt tcatcgccca gaagctgata ggttgggcag gttgcggtac aaaatccgca
    10921 gtgaacacag gcgcgcagga tgctgtcggc ttccagcgcc cgcgcgttct gccgcatctc
    10981 ttcagttaat tgggtttgca tagcctgctc ctcaaagttc cgcgtacatg cgaccggggt
    11041 taaacacgcc gcaagggtcg agctgctgtt taagctgctg gtgatagcgg aataaaggag
    11101 ccggtagcgg ggcaaagcca ccctctccgg cactaaagcg ggtcgcatga ccgccagcgt
    11161 tgcgggcgat gcgatggatt tgattgtcat cggctgtcga tttcagccag cgtaacgccc
    11221 cgccccagtc gatcagttgc tcgccgggta aatccatcat cggcgcatca ctgggtaatg
    11281 aaatgcgcca taaggtacct ggtaacgaga agaacggcag ttgttgttca cgcaattgct
    11341 gccagaactg acccgcaacc tcttcgcccc ccagcagttc acgcgccgct tttaccgatc
    11401 cttcgccgcc ctcaaggcgg atccacaacg cattgtcgaa gtaacataag ccactaatgg
    11461 gtagcggctg gagttgccac tgggcgattt cgttcatggc ttcttgcagg ctgatttccc
    11521 gacgcaggct cagggaggcg cgcggtcgcg gtaacacttt catcgagatt tcagtgagca
    11581 cgccaagaca accgtagctt ccggccatta accgtgagag atcgtatccg gcaacgtttt
    11641 tcatcacttc gccaccaaaa cgcagatgtt ttccagcgcc ggtaatgatg cgcgtgccga
    11701 ggacaaaatc gcggaccgaa ccgctccacg ggcgacgcgg ccccgccagc ccgcaggcga
    11761 ccatcccgcc ccaggtggct tcttcaccat aatgcggcgg ctcgcagggg agcatttgcc
    11821 cggcgctctc cagcgccgct tcaatcgcca ccagcggcgt tccggcacgc gcggttatca
    11881 ccagctcggt cgggtcgtaa ttaacaatgc cgcgatgaca acgcacatcc agcgtttgcc
    11941 cggtgacagg gcgacctaaa aaggctttgc tattgctgcc ctgaatcacc agcggcgttt
    12001 tatcgctaat tgcctgattc acctgctcca gcagcgcctg gctgtaatca cactcgcgta
    12061 gcatcagaaa cgctccagtt cagggaaagg taaatgaccg tgatgcacat gcatggcacc
    12121 aaattcagca cagcggtgta gcgtgggaat gtttttccca gggttcagca aaccatcggg
    12181 gtcaaacgcc gccttgaccg catggaaggt cgtgatttca tcgctgttga actgggcgca
    12241 catttgattg attttttctc gcccgatgcc atgttcccca ctgatgctgc cgccaacttc
    12301 aacgcagagt tcgaggatct tcccacccag ctcttccgcg cgggcaaatt caccgggttc
    12361 gttggcatcg aaaaggatta acgggtgcat gttgccatct ccggcatgaa agacgttggc
    12421 aacacgtaaa tcatattgct gcgataaacg ggcaatgcct tccagtacgc caggcagggc
    12481 gcgacgcggg atggtgccat ccatgcagta gtaatccggg gagatacgtc ctaccgccgg
    12541 gaacgcattt ttgcgaccgg cccagaaacg tacgcgctct gcttcgtcct gtgccagacg
    12601 gacgtcagtc gcgcccgctt tcaacaagat gtcgttaacc cgctcgcagt cttcctgtac
    12661 gtcagactcc acgccgtcca gctcgcataa caaaattgct tcggcgtcga cgggataacc
    12721 ggcatgaata aaatcttccg ccgcgcggat cgacaggtta tccatcatct ccagcccgcc
    12781 ggggataatg ccattggcga tgatgtcacc aactgcaagt ccggcttttt ctaccgagtc
    12841 aaagctggct aacagaaccc gcgccacggg cggcttcggc agcagtttta ccgtcacttc
    12901 ggtggtcacg ccgagcatac cttccgatcc ggtgaacagc gccagcaggt caaaaccagg
    12961 tgaatccagc gcgtccgatc ccagcgtcag cgcctcgccg tccagcgttt gcacttcaat
    13021 tttcagcagg ttatgtacgg tcagaccata tttcaggcag tggacgccgc cggcattttc
    13081 tgccacgttg ccgccaatgg aacaggcgat ttgtgaggaa gggtccggtg cgtagtagag
    13141 attatgcggt gcaacggcct gggagatcgc caggttacgc acgcctggct gcacgcgcgc
    13201 gcggcgacca acggggttaa tgtcgaggat ctctttaaag cgcgccatca ccaacaacac
    13261 acctttttcc agcggcagcg cgccaccaga aagcccggtg cctgcaccac gggtcaccac
    13321 cggtacacgc aggcgatggc agacagcaag aatcgctgtc acctgttcca tttgcttagg
    13381 cagaacaacc agtaatggac gcgtgcgata cgcgctcaac ccgtcacact cgtaaggaat
    13441 gatctcctca tcggtatgca ggatctcaag tccagggaca tgctcacgca gtgccatcag
    13501 taccgatgtg cggtcgacat cgggtaaagc gccatcaaga cgctcttcgt acaagatgct
    13561 catgagtagg cttcgctttg ttgtgttgtg tggcagctga tttttgcgcg ctgcttctgt
    13621 gaacagttat taagcgggct tttcgttttc gtctatctct ttagctaccg gtcagaccat
    13681 tttttttcca gctctgtgac cttgccttgg ttaactcaat gttaaattga tgtaacataa
    13741 tcacttacgt gatgtgcgtg ttttgcgagt taagaacaga aaaattggtc ctacctgtgc
    13801 acgaggtccg ggaatgaaag atgaacgtcg ccctatttgc gaagtggttg cagagagtat
    13861 cgaacggtta attatcgacg gcgtactgaa ggtcggtcag ccacttccct cggaacgtcg
    13921 actgtgtgaa aagctcggct tctcacgctc cgcactgcgt gaagggctga ccgtgctgcg
    13981 cgggcgcggg attattgaga cggcgcaggg tcgcgattct cgtgtcgcac ggcttaatcg
    14041 ggtgcaggac accagcccgc tgatccatct gttcagtacg cagccgcgaa cgctgtacga
    14101 tctgctcgac gttcgcgcat tactggaggg cgaatcggca aggctggcgg caacgctggg
    14161 aacgcaggct gattttgttg tgataacccg ctgttatgaa aaaatgctcg ccgccagtga
    14221 gaacaacaaa gagatttcgc tgatcgaaca tgcgcagttg gatcacgctt tccatctcgc
    14281 catttgtcag gcttctcaca atcaggtgct ggtgtttacg ctgcaatcat tgaccgatct
    14341 gatgtttaat tcagtgtttg ccagcgtaaa taatctctac catcgaccac agcaaaaaaa
    14401 gcagatcgat cgccagcatg cgaggatcta caacgcggtg ttgcagcggc tgccgcacgt
    14461 cgcccagcgc gcagcacgcg atcatgtgcg gaccgtgaaa aagaatctcc acgatatcga
    14521 gctggaaggc caccatttga ttcgctcggc ggtgccgctg gagatgaacc tgagttagct
    14581 ggtattaaat ctgcttttca tacaatcggt aacgcttgta cggctccgcc ccaatgcgtt
    14641 ccagcatgtt attcatgcct gtattggttt cgaggatcca tgacatctcc agcgcatcga
    14701 tcttccggcg ggcaaacgga tcgcgtaagg cttcaatcaa taacagcgca atcaccgggc
    14761 cgatgcggct gaactgatac tcgtcgcgca cgcccatcag cggtactcgc gcagttcgca
    14821 caccgctgac tttcaaacgc cacagcaatt ttgcccagcc gaagggaaag agcgatccgt
    14881 tcagatcggc aatcgcctcg ttgatgttcg gcaagccgac aataaacgcg cagggtgcag
    14941 aatcaatctc agcgatatag atcatatcgt ccggcaccag atatttaagt tgatcgccca
    15001 tggtcgcgaa ttcatgttcg gtaaacggca caaatcccca gttgtgctgc cagccagagt
    15061 tgaaaatctc acgcaggatc tgcatttctt cggcaaaccg ctgacgattg atgcagcgaa
    15121 tggtcacctt tttgcgcacc tgatccatca gtttttttag cgccggagag aaagtgagat
    15181 cggttcgctg catccaccac gccagtaaat caatgccttt gtgataaccc agttgttcaa
    15241 tatgcgcggc ataccacggt ttgccgtgtg gcatcatcgc acagggtggt gtgtcaaaac
    15301 cttcaatcag taatccgctt tcctgattga tattcaggct gaaaggaccg ctgatcttac
    15361 ttgcaccttg tgacttcaac cacgcttccg ctgcgccaaa caacgcggca aaaacctgcg
    15421 gatcatcaat ggcgtcaatc atgccgaaat gaccggtatc ttcaccgtaa cgctcacggt
    15481 gcaaggtatc tatttgcgcg gtaatacgcc caactatctg ccccgctttt tttgccaccc
    15541 acgcctgcca gatgatatgg tccgtccccg gatttttcgc agacaaatgc tcgttgcgtt
    15601 ctatgaataa agggggtatc cagtttggat cgtcgggata aagtgatgac ggaaaagcga
    15661 taaatgcctt aaggtcattt ttattaatta ctttttcaat tttaatcatc agcagatcgc
    15721 attccatatc tctgtggata acaaataatt aacggtgaaa aacaggttgc aacagcaacg
    15781 aacgacacct taataatcaa cccaatgaat acaaacaaag aataaatagt atttattaat
    15841 gattatgata aggttagaga ctacacagaa gcgcctcaat gccttttgtc gatttaaggc
    15901 gaccggacaa ggcttcttct taatatattc ataaattaaa tatcattcac aataaaaaca
    15961 aaaaacttaa actcattatt taaatcaatg ttcattttca caccgatgaa aatactatct
    16021 catttatctt ttatgcctat ataatccacc gcattattgt cattttattt ttgatatcca
    16081 attttttcgt ggtgtatatg tctaataaaa tctttacgca ttccctacct atgcgctatg
    16141 ccgattttcc aacgctggtt gatgctttgg actacgccgc tctgagtagc gccggaatga
    16201 atttttatga cagacgttgc caacttgaag atcaactgga atatcagaca ttaaaaacgc
    16261 gtgccgaagc tggtgcgaag cggttgttat cgctgaacct gaaaaaaggc gaacgcgtgg
    16321 cgctgattgc cgaaacgagt agcgggtttg tagaggcttt ttttgcctgt cagtatgctg
    16381 gcttagtcgc cgtcccgttg gcgattccaa tgggcgtcgg tcagcgggat tcctggagcg
    16441 ccaaactcca gggtttactg gcaagttgcc agcccgcagc cattatcact ggtgatgagt
    16501 ggttgccact ggtcaatgcc gcgacgcata acaaccccga attacatgtt ttaagccacg
    16561 cctggtttaa ggcattaccg gaagccgatg ttgcgctcca gcgtccagtt ccgaacgata
    16621 tcgcctacct ccagtacacc tccggcagca cccgttttcc ccgtggcgtc attatcaccc
    16681 atcgcgaagt gatggctaat ctacgtgcta taagccacga tggcattaaa ttacgccctg
    16741 gcgaccgctg cgtctcctgg ctgcctttct accatgatat gggactggtc ggctttctcc
    16801 tgacccccgt cgccacgcaa ctttcagtag attatttgcg cactcaggat tttgccatgc
    16861 gtcctttgca atggcttaaa ttgatcagta aaaatcgcgg caccgtttcc gttgcgccgc
    16921 cgtttggcta tgaattgtgc caacgccgcg tgaatgaaaa agatctcgct gaactggatc
    16981 tttcctgctg gcgcgtcgct ggtattggtg ctgagccgat ctccgcagaa caactccatc
    17041 aattcgctga atgtttccgt caggttaact ttgacgataa aacgttcatg ccgtgctacg
    17101 gactggcaga aaatgcgctg gctgtcagct tctctgatga aggctctggg gttgtggtta
    17161 acgaagtgga tcgcgacatc ctagaatatc agggcaaagc cgtcgcgccg ggtgctgaaa
    17221 cacgcgccgt atcgactttc gtcaactgcg gcaaagcgtt gccggaacat ggcattgaaa
    17281 tccgcaatga agcaggtatg ccggtcgcgg aacgtgtggt aggccatatt tgcatctccg
    17341 gccccagcct gatgagcggt tactttggcg accaggtttc gcaagacgag attgccgcga
    17401 cgggctggtt agacaccggc gacctcggtt atctgctgga cggttatctg tatgtcaccg
    17461 gacgtattaa agatctgatt attattcgtg gccgtaatat ctggccgcag gatattgaat
    17521 atattgcgga acaagaaccg gaaattcatt ctggcgatgc gattgctttt gttaccgtcc
    17581 aggaaaaaat cattttgcag atccagtgtc ggatcagcga cgaagaacgt cgcgggcagc
    17641 ttatccacgc gctggcggca cggatccaaa gcgaatttgg cgtaaccgcg gctatcgatc
    17701 tgttgccgcc ccacagtatt ccccgaacat cctccggcaa gcctgcccgt gcggaagcga
    17761 aaaaacgtta tcagaaggct tatgctgcaa gccttcatgt gcaggaatcc ctggcatgaa
    17821 tcaaactgtc gcggtgacgg gcgctaccgg gtttatcggt aaatatatta tcgataacct
    17881 gctcgcccgc ggctttcacg ttcgcgcatt gacgcgtact gcccgcgctc acgtcaatga
    17941 taatctcacc tgggtgcgcg gttcgctgga agatactcat tcacttagcg agctggttgc
    18001 cggagccagc gcagtggtcc attgcgccgg gcaagtgcgc gggcacaaag aagagatttt
    18061 cacccgctgt aacgttgacg gcagcctgcg cctgatgcaa gcagcaaaag agagctgctt
    18121 ttgccaacgt tttctgttta tctcttcgct ggcggcgcgc catcccgagc tctcctggta
    18181 cgcaaattcc aaacacatcg ccgaacaacg gctgactgca atggctgacg aaattacgct
    18241 gggcgttttt cgcccgacag ccgtgtatgg tcccggcgat aaagagttaa aaccgctgtt
    18301 tgactggatg ctgcgcggcc tgctgccacg acttggtgca ccagatacac agctctcttt
    18361 cctgcacgtc accgattttg cgcaagcagt gggtcagtgg ttaagcgccg aaactgtaca
    18421 gacgcaaacc tatgaattat gcgatggcgt cgctggcggc tatgactggc aacgcataca
    18481 gcaacttgcc gccaacgtcc gttgcggttc cgtgcgaatg gttggtattc ctctgccggt
    18541 actcacctgc cttgcggata tcagtaccgc gttgagtcgc ctggcaggta aagaacctat
    18601 gctgacccgc tcgaaaattc gtgaattaac ccacgccgac tggtcggcaa gtaataaccg
    18661 tatttctgaa gatattaatt ggtttcccgg gattagcctg gaacacgcat tacgcaacgg
    18721 gctattttga ttgtcttgtt taaaggtgtg ttaacgtttc tatggtaaat cgtgaaatag
    18781 taatggatta catcctttcc tgtttacagg atttggtcga aaacggtgtg gaaattaagc
    18841 ccgacagcga tctcgtcaac gatcttggcc tggagtcgat caaagtgatg gatctcttaa
    18901 tgatgctgga agacagattc gatatttcca ttcctattaa tattctgctg gatgtcaaaa
    18961 cccctgcgca attaatggaa actctacttc catggctgga gaataaataa tggggctata
    19021 cgataaatat gcgcgccttg ctggcgagcg actgcaattt agtgacaacg ggttaacacc
    19081 gtttggcacc tgtattgatg aagtgtattc cgccaccgaa ggccggatcg gcaataaaaa
    19141 agtgattctg gccgggacca ataattatct cggtttaacc tttaatcatg atgccatcag
    19201 cgaaggtcag gccgcgttag cggcccaagg taccggcacc accggttctc gtatggcaaa
    19261 cggcagttat gcgccgcacc tggcgttaga gaaagagatt gccgagtttt tcaatcgccc
    19321 taccgctatc gttttttcaa ccggttatac cgctaattta ggcgtcatca gcgcacttgc
    19381 cgaccataac gccgtggtgt tactggatgc cgacagccac gccagtattt atgacgcctg
    19441 ttctctcggt ggcgcggaaa ttattcgttt ccgccataac gatgctaaag atctggaacg
    19501 gcgcatggtt cgcctcggtg aacgggcgaa agaggcgatt atcatcgttg agggcatcta
    19561 cagcatgctc ggtgacgttg cgccgctggc ggaaattgtt gatatcaaac gtcgtctcgg
    19621 tggttatttg attgtcgatg aagcgcattc gtttggcgtg ttaggcgcaa cggggcgcgg
    19681 actggctgaa gctgtcggcg ttgaagatga tgtggatatt atcgttggta ctttcagtaa
    19741 aagtctggcc tcgattggcg gttttgctgt cggttcggaa gcgatggaag tgctgcgcta
    19801 cggcagccgc ccgtatattt ttaccgcctc tccttctccg tcctgcattg cgaccgtgcg
    19861 ctcatcgtta agaaccattg ccacacaacc agagctgcgg caaaagctga tggataacgc
    19921 caaccatctg tatgacggtc tgcaaaaact gggttatgag ttaagttcac atattagccc
    19981 ggtagtgcca gtaataatcg gttcgaaaga ggaaggttta cgcatctggc gcgagcttat
    20041 ctcgctgggc gtgtatgtga atcttatcct gccgcctgcc gcgcctgcag gcatcacgct
    20101 gctgcgctgt agcgtaaatg ctgcccacag tcgtgagcaa atcgatgcca tcattcaggc
    20161 atttgctact ctgaagcagt aatgtgagcc cgccgtggtg tttgcgccac ggcgggttta
    20221 atgttgtctg tcataccaat acaccaggct caacgccacc aacacaaacg ctaccggtaa
    20281 accgagcgcg gtaacaggtg cgcttaacgc aaatagcaat cccaaattga ccatgatttg
    20341 atagctgatc caggtgagta agccaacgat gacgcctaca gcgagacggc tacccatccc
    20401 tggcgagcgc ggggcgctaa aggtaaacgg cacagccagc aaaatcatcg ccagggtcag
    20461 gatcggttgc cccagttttt cccacagtgc aaggcggtat tcgctgctgg gttgcccggt
    20521 ttcctgcaaa taatggatgt aatgattaag ctgacggaca gaaaaagtat tccccggcat
    20581 cgataactca tcaagatcca tactggtgaa ggccgattgc caggcaagat tatctgatgt
    20641 ttccagcgtt tctttaccgt taagccattt tttatgattc acaccatgca ggatccacgt
    20701 tttgtcgtct ttaatggtgg cactgcgtgc ataaatgtag gattcgagcg aaagatcgtc
    20761 acgataatga aaaatctcca cgcccacagg ctggccctgt tcattcagcg atttcaccgt
    20821 cacaaattca ttgccgcgcc tggcccacag catattgccg gtaatgtcat cgtcctcccc
    20881 caacgcggtg gcggtcgatt tgatttgcag cgcctgttgc tgcaatggcg acgccaccca
    20941 ctcatctatc gcgcctaacg aaacagtcca caatatcccg gcaaccagcg ccaccagtgc
    21001 aatacggaag atagaaaacc ccgtgctgcg aatggcggta agctcactgt ttttcgataa
    21061 ctgccccagg ccaacaatcc ctcccaataa ggcgataaac ggcgaaagtt cgaccagtgt
    21121 gcgcggtaag gtcattagca ccaccagcac cgcctgagtc cagcgataac cgcccgggct
    21181 gacatcatcc agttcgttaa tcaggttgaa ggtggtaaaa agcggcagca atagccctgc
    21241 ggcggcggca aaaccgagaa agagatgacg gattaaatag cgactgaaaa cattcatcgg
    21301 gaaaatttct gcagcaaaga gaagtcgcgt gcgaccagca tcagcaggcc cattagcatg
    21361 agtccgggca ctaaccacaa accaggaatg agaggaatcg cgccgttagc caccagcgta
    21421 cggcagacgt cgccgccata aaaaatggca ataaacagca acgtcagtgg caataacgtt
    21481 gaaaatcgcc cttgccgcgg ttttacccga cttaatgaaa tggccagcag cgccatcaac
    21541 aatgcggtca ggccacggct ttgtcgccat tgcagttcgg catgatcggc aggaaaggcg
    21601 gagcgcgcca gctccgtgac cgattttgct ttgcgtttga cgttagggct ttgatccagc
    21661 ggattcagat gtaattgcag attacggtag atctgttcgt tgtcgtcacg cccctgatga
    21721 tcgagaagat aggcagtgcc gttgtgcaac ataacggtcg gtttctcggg tgatgggtca
    21781 accacatcaa ccgaacgggc gcggaaaatg cgggttcgat tggcagtaga agtataaatc
    21841 agcgcgtcag tcaggcgatt attatcctga tcaaccgtct gcgaaaggat cattcgtccg
    21901 ttatcgttag tgttaaattt ctttgcccgc aactggcgaa catccagctc cgactgcgac
    21961 tgctgctcca gttgataaat ctgcgtataa gcccacggac gaccatacat cgacagaagg
    22021 gtgacaaaaa tacttagcgg tatcgccaga taaagaacgg ctttgtacaa gcgtcccgga
    22081 ctgccccccg ccgcagagat agcggtaatt tccgagtcgg tatacatctg ccctagcgtc
    22141 acgccaactg acacatacag accaacaggt aacaacatct ccagtgcaat cagcactttg
    22201 tagaaaacga tatccagcac aacatcaaga gctaatgtcc catttgccgc ttcggtcaga
    22261 taacgctgtg cggagtagct ggcgaaaata aagatcagaa aaccgacgat aatcagcacc
    22321 agacggcgcg taccacgcat gatgtagtgt tcaaccagct tcatctcact aacgccctca
    22381 atcctttgaa ttttttattc attgatcagt ttctgcgcca gcgcaatgac atgatctacc
    22441 tcttccggcg tcaggctgcc gtcctttgcc cactgaattt gcccggcttt attcagcacc
    22501 agaatggttg aactctgctc cggcaagcgc caggcaacgc gtccctgtcc attgccgtca
    22561 ataatgaatt gcgcccacgg atagcggcgt ttattttttt cgattttgcc aacaacaaaa
    22621 tagccagtac caaagatggc atcatcggtg ttcacaatgg tggtgggctg aaaacgatcc
    22681 tgcggaaagt ttgccgcttc aaccgcttta attaacagtg agttcttttt cttcgccgac
    22741 ttacgcccgg cgatatactg aataatgcgg acttttcctg ctaattgtgt gctgttccac
    22801 ttttggtagt gaatttcgtc gtcgctattc agaagcagct cgccgccttc ctggatataa
    22861 acaggggtaa ccgttttgcc agtgacgaaa ttgtgtgcct gcacacccgg ggctaagagc
    22921 atgataatca acgcaataat caaacgagat gacatcattg ctccggctat agcggaaaac
    22981 gcgaaatcag cagctctaag cgctgacgaa tatcgctatc cagcgccaac ggtgcatcat
    23041 acgactggcg gttttccata tcatccgcca ccatgtcgtc gtgtttatct cccatcaggc
    23101 cattaatggc ggcgaggaaa cgggaaatct gtaacgcctc tttattttca ggatcaagcg
    23161 atcttaaggt gtcgtaataa gaaacctgtt ggctgatgat ttttcgcaca tcatgaaatt
    23221 ccctgccggt tagtcgcggt ttggcaataa tctctttcag ggtatgcatt tgcttttgcc
    23281 gataggcaat aaaactgctt acgctttcta actggctatg gcgtaatgaa tagttcacca
    23341 acgaccagac cggagagctt aaataaatcc gtaacaggtt gccgtaatac gacacaatat
    23401 ttttcttacc gttacgaaag ccgtcctgaa aacgccccag aaaaacggtc gttttgccga
    23461 ataaaaatcc ggcctgatgc ttctttaaat acagccgttg cgcaaaacgc aggtgcttgt
    23521 agcgcgctcg catgtattta aattgtttgc gctcatcagc agtcagctca tcgcctttgg
    23581 cttgtttgcg aactaaacgc agtaactcct gacgattaac gccattgagc cagaattgca
    23641 ggcataacgc gtaattatca tgaatgattt cggtggtaca acttagtgca atagtcggcg
    23701 gcaaactaac ctgtgcatca acgacatcat cttcttcaat cgcaacaaat agacgctcgc
    23761 tcgcctcaag cgggaaatat tttctcatca tcaggatttc tctccggctg cgttataaag
    23821 caaggaatgg cgctgaaaaa atgcctttaa ggttggaatt aacgagagcg ccacgtccag
    23881 acattgccca ccgaagtaag caaacgctgc tcccagcaca ccaaacagat gacagaacat
    23941 gaacagcaat acgatgtagc cgattgaggc gatggtttgc gccacgagaa aagcgcgctg
    24001 ttttcccgcc atcaatagca atgactcctg agggaagccc agcatcgaaa tcacaattgc
    24061 gcccagcatc acctgaatca ggtcatacgc ttcgagatac ttaacgccaa acaccagcga
    24121 aatgagcggc ttgccgacga tcaacaccgc gagcgccacc agaataccga ttccgcccgc
    24181 cagtaaaccg gacttcacac ctaacaacca cggtctggtg gtgcgcggat ctaaacgcat
    24241 cacctctggg taaaaacttt tacccagcaa acccgctggc gttccggcgg cgtcgaagaa
    24301 tgtcatggcg attttaaata acccggcggc agcgggtcct aacacgatcc ccaccagcac
    24361 tgtgctgcac gagttacgcg ccgaccagat ggagtgggca atgttggttg accagacaaa
    24421 actccacgcg cctttgatgt gtcgggcaga ctcaaacaga ttcaatttga aggcattatg
    24481 gatatttctg cggcgtaatt cgcgcgctgc aaaccaccag tacatggtgc cgccaaccag
    24541 attcgacacg taccaggcaa taacaaaacc cgcaaaacca aagtcaaaat accaggctac
    24601 gacgctcccc gctgcgcgca gaaaaggttt cgtcgcctgc tgtacagcaa ttaaatcgaa
    24661 gcgatctacc gcacgcagaa tgccggtcgg cgtggaggaa gccattgaag gaatgagcgt
    24721 gcaatacagc gccgccagcc aaaagctttg gtcatccaga cctaatgaat gggaaaggaa
    24781 tggcagtaag gcaatgccgc cgacaatcgc caccgcgccg ctgacgatat ccagcgagaa
    24841 ggaaaatgag acgacattgc gaaattgctg cggattattg ttggtcaagg ctggcgttcc
    24901 gtactgaacc accagctgcc atgtctgaaa cttaataaaa tcgctgatcg acttggcgta
    24961 cgattgcaca atcaccagta cgccaaacat ggcgggcgtc atccctttac cggcacacga
    25021 gagcgccaac agccccagca aggcgctcac gacattactg gagcctaacc aggcgctatt
    25081 gcgaataatg gtgcgaaacg cgccatctgc aaaccaatgt ttgatgttaa aacccgccaa
    25141 ttcagcctga ccttatcgtt gatagtaaaa agtatcccgc cagccttaag ttaaacttcg
    25201 gcggtcagaa acgatggcaa ccagagaaac cgccttctgt gcctgttcca gcacttcgct
    25261 gtagggcgct ctggaatcaa tctcaagaat ttttgtgccg ttatagccaa tcgtcgacat
    25321 gacgccgatt ttgtcctgca gctcggcata gtcatggtca ggcttgcggg agatggcagt
    25381 ctcaatatca atgcccaggc gaataattaa ttccgggcga tattgcgcca tttgttggta
    25441 taaacgccgt tcgcgctgcg ccagaaacat gctgattttc ccggtcgcac gttcgacgcc
    25501 aatccccggt ccgtcataat aaaagcccga aatttcagcc tgcgggaagc gatcgctgac
    25561 caccagaacg ccactttgcg ccagtcgctg aaccttgcgc agattcgcca ttcttcgcag
    25621 cgagaagcag tacataatca ccgccgccca tagcgccgga gatttggttt tcatgctttg
    25681 ggttttcgag gatttggccg ccagtcgccg ttccagccag acgccaacca acggcaatcg
    25741 tttgattttg tcgccgtctt cgccggagag cagccccaaa tagcggcgct cggtttgcca
    25801 gtgttgttgc agcgatttca ccaggtcggt ggtcagcgtg gatttaccgg tgccatcaca
    25861 cccgaccacc gcaatcagcc ccggaatgta gttgggctgc ggtgcggttg tactattaac
    25921 agtttgtgtt tgtagtgcat ccattcgtta aatcatcccc tatccatttt ggtgatcagg
    25981 aaagcgcggc gtgaattgcg cgtagtgacg cttgcataat ttcatcggct ggttgccgac
    26041 catccagttc aaggatcttt gcgccattga atgtcagttg cggcgtaacg gcgatttttt
    26101 cctgtaacgc tgccagttgg tggtcaggtt tacgcgcaaa cgcggtttgt tcatcaatgc
    26161 caagacgaat caacaatacg ggcaaataag acgccatcca ttggtacagc ttcagctcgc
    26221 gctgccttaa catttttatc caaccgttac cgcccgtggt ttttgccaat tgcgggccat
    26281 caaagcgaaa ccccggcact tcaacctgcg ggtagcggtc ggtgatgagc agaaagcctt
    26341 gctggctttt acacaacatt ttgcgaaact tgtacgcccg ccagcaggaa agcagataga
    26401 taaccagcgc agtaatattg ccaggcggtg ttgagggctt ttcgtgcaca tgtgccgctt
    26461 tacttcgcag ataacgccca aaaggtgcgc caataacagg gagctgtgaa atccattcgc
    26521 caattcgccc ggacgattgc ccgagataaa tgcgttctgt tggcattctt gctgccagtt
    26581 catttaccag gcttgccgtg agggtcgatt tacctgaacc atcacatcct acaatggcaa
    26641 taacacgcac tggcgtggaa ttaattattg acatattacg ttgattcacg aaaaacccgg
    26701 cagtaaataa tgtattgaat attacgtggt ccgtgctcag aatatccgtt caaccttatt
    26761 gtgacaatgg ttaattgtga cattgacctg aattacgctt tcataaaaac atattaacca
    26821 aataaatatt ttggatggat atttaaattt aaggatatat tcatgcagtc aataacacct
    26881 ccattaattg ccgttattgg tagcgatggt tcaggcaagt caacggtgtg tgaacatctc
    26941 attaccgttg tcgaaaaata tggtgctgcc gaaagagttc atttaggaaa acaggccgga
    27001 aatgtcggtc gtgcagtgac aaaattaccg ttgatgggaa aatccttaca taaaacaatt
    27061 gaacgaaatc aggtgaaaac agcaaaaaaa ttgccaggac cagttccggc gctggtaatt
    27121 acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata tgcttgcctg tcgtcgtcgc
    27181 gggttaattg ttctaaccga ccgttatcct caggaccaaa ttcctggcgc ttacgatggt
    27241 acggtgttcc cacctaacgt tgaaggtggt cgttttgtct catggctggc aagccaggaa
    27301 cgtaaagcgt ttcactggat ggcgagccat aagcctgatc tggtcatcaa actcaatgtt
    27361 gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac gggaatcgct ggcgaggaag
    27421 attgccataa cgccacagtt aacctttggt ggtgcacaac tggttgatat cgatgccaat
    27481 cggccactgg aacaggtgtt ggttgatgca gaaaaagcga ttacggattt tatgaccgcg
    27541 cgtggttatc actagtcaaa aatggaaatg cccgatcgcc aggaccgggc attttcagga
    27601 aggttaaatc aactgcaatg ctatccagta cagcccacca gaaagaaaaa ttgccgccgg
    27661 taaagtaaac acccacgcca tcaggatgct ggttaccgtt ttacgctgta acccaccgcc
    27721 gtccaccacc atcgtccctg ctaccgcaga cgagaggacg tgtgttgtgg agacaggcat
    27781 cccaatataa ctggcaaggc cgatagacac tgccgccgtc atttgtgccg ccatgccttg
    27841 cgcatacgtc atgccgcgct taccaatctt ctcaccgatg gtcatcgcta cacgacgcca
    27901 gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc atgatgatcc acaccggagc
    27961 gtactcaatg gtgcttaaca tatcgctgcg cagttttttc agcaggttct ggtcttcttt
    28021 actgacgcct ggcagtttcg ccagcttcgc ggaggtatca gagatgcaca gcataatgcg
    28081 gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa ctttccatat tgcctggcag
    28141 catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc ggatgacagt gaaactctgt
    28201 tacttgcgtg ccatcagtcg atgctgcagg caatggaggt tccatcgcaa tcaacttctg
    28261 cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg gtaacggcat cgcgggtacg
    28321 ggtaatttca tagccggacg cattcatatt gacgacgaag ccagcagggg caatccccac
    28381 cagtaccagc attaccaggc cgatcccttt ttgtccgtcg ttcgcgccgt gcgaaaacgc
    28441 cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag aatggcggtt tacgtttgcc
    28501 ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt tcgtcccgct
    28561 ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg accaggccga cgatagggga
    28621 aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg tttaacgcat ccatcaccga
    28681 tgagccggtt aacagcgcgt tggttaaacc gatgccgata atcgcaccaa tcaaggtgtg
    28741 cgaactggag gccggtaaac cgaagaacca cgttcccagg ttccagataa tcgccgccag
    28801 cagcatggaa aagaccatcg ccagaccgtg ggttgacccc atattcagca acaaatcggt
    28861 tggtaacata tggacaatgg cataggcaac gctaagtccg cccaataaca cgccaaaaaa
    28921 gttaaaaaat gccgccatca ccacagcaag ttgtggttgc atggcacgag tataaataac
    28981 ggttgccacc gcattcgccg tgtcatgaaa accattgatt gcttcgtaga acaacacaaa
    29041 tgccagagca agcaataaca aaagccctgt gtatatatca aggccaacaa ataaatttag
    29101 catatattag attacgccat tttgaattta cgaacggacg cattatcagt gactttaacg
    29161 gcatgggcaa agtgaaatat cattttttaa cgataaaaag agtgttattt cttgtgtatc
    29221 agacaataag cattcattca aaacctttag aaataatcaa aacacaggta ttccactggt
    29281 taatatattc acatatgaaa tgaataaata ttagaacata taaatattga atattttgat
    29341 taatccccgt actgattatt cttcataatc agtacggggt tgctacaaca tgtattactt
    29401 tttcaccaca attaacggtt caatatcact ctcttttttg atgaccagtg attcatcacc
    29461 gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag gtacatacct gaatgtattt
    29521 accgtccact ttcggcaaac accacagttg ctgatagatg tttttctgct cggcaaattt
    29581 accgctggtt ttgtccagca cctcttcatg atggctgacc aggtcgatat tgctaccaca
    29641 gcgaccggca atcggtttca ctgcgtaacc tgttttcacc aattcatcat taacagtgaa
    29701 atcggtatcc agcaggtaac gatggtgcgg gaacagcgac cagaggatcg gcagaatcgc
    29761 tttgttgccg gggatcaccg tccacagcgg ctcaaagacc agcacttccg ggcgcagcaa
    29821 tacgtcgata agacgcactt cgttttgcgg atgaccggta cggattggca ccgcagcaaa
    29881 ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt tcccacgccc aggttttcca
    29941 cacgcagtta accagccgtc cttccccatc aatcagttgc ccggcagcat cccagcccag
    30001 ttcatccagc ccgcgcaaga tacgcgtttc aaagcccgcc tggtgcagcg cctgctccat
    30061 aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc atgatatgga caaacggacg
    30121 tgcacgactg tgtttccagg caccagccag ttcgttaatc agcccttccg ccggattgaa
    30181 gccgttgcct ttatagccct gctccgccca acgttcgagg atcaagcccg cttcggtatg
    30241 acaggaggcg gagtcggcgt tgtactcgta aaccttcagg ccacgctcat ccatgcagaa
    30301 atccatacga ccagtgatca tatggtgacg gcgacgctgc caggagagac gcaaacgtgg
    30361 ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg tcatctttca gcaccttgtc
    30421 ggttgcgtga agatacatca ggtgcagctc gttggtggct ttaattagct cctgctcggc
    30481 actctcggta atggtgtagt aatgataagg atcctgattg atcacctgac cgttggcctg
    30541 cacataggcg ttttgcagcg gatctttttc atccagccat ttaccgtcaa actggccttt
    30601 gttttccagg cgcgctccgc tgattttcag cagctcgcct gcaatttccg gctgcggtaa
    30661 gctgtattca gtatcttccg tctggatcat ccagcccaga atggtggtgt catcaaaagt
    30721 gtctttcagg gtatagcagc cgttttcgac caccatctcc agctcacgcg tccactgttg
    30781 cccttgcggc aacggggaat gaatcacgtt ctgttccgca atacggactt tgttgccatg
    30841 caattgggta atgatggcga catggccagt gtctttaaat tcaccgcctt tatcccagat
    30901 aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta ggaaatgcct gcaatggcag
    30961 gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag aaaatctccc acgccatacc
    31021 cacgtcagta aagaccacac cgtaattcag aaagagaaaa cggcgagcaa attcaacgca
    31081 ttgccacttg tggcccatat attcgtcgtc gatatagcta cggaatacgg cgtcatcttc
    31141 gtattcctgc ggatcgagag aactgtaatc tgaagagtag attgctaccc cacctggggc
    31201 gtagcccaat aatgtcccga acggggcatc ctggctggtc gttcctttgc tcatcacttt
    31261 acctttaaca atacaaccta agcagttggc gtaattgttg tgctctgatt acctgctcag
    31321 cgacattaac cggacagagg tcagactaat catacacctc atcgcagcgg ctggcgcaaa
    31381 gggttaaaaa attcacattc tgtacagcaa gtgacctgct acactgcttc aacactacca
    31441 ctcagaaggc aactcactat gacagacaat acttatcagc ccgcgaaagt ctggacgtgg
    31501 gataaatccg ctggcggcgc gttcgccaat atcaatcgcc cggtttctgg tccgacgcat
    31561 gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt attcgctggg aacgccgaac
    31621 ggtcagaaag taacgattat gcttgaggag ctgctggcgc tgggcgttac tggtgcagag
    31681 tacgacgcct ggctgattcg tattggcgat ggcgatcaat tctccagcgg ctttgtcgaa
    31741 gtgaacccaa actcgaagat cccggcgctg cgcgatcata cgcataatcc gccgatccgc
    31801 gtgtttgaat ctggttcgat cctgctttat ctggctgaga aatttggcta cttcctgccg
    31861 caggatttgg caaagcgtac tgaaacgatg aactggctgt tctggttaca gggcgcggca
    31921 ccgttcctcg gcggtggttt tggtcacttt taccattacg cgccgatgaa aattgagtac
    31981 gccatcaacc gctttaccat ggaagccaaa cgtctgctcg acgtgctgga taagcaactg
    32041 gcgcagcata agtttattgc gggcgatgag tacaccattg cggatatggc gatttggccg
    32101 tggtttggca acgtggtgtt aggtggtgtg tatgatgccg ctgagtttct tgatgcgggc
    32161 agttataagc atgtacaacg ctgggcgaaa gaagtaggcg aacgtccggc ggtgaaacgt
    32221 gggcgtattg ttaaccgcac caacggaccg ctgaatgagc agttgcatga gcgccatgac
    32281 gccagtgatt tcgagacgaa tacggaagat aagcgtcagg ggtaagggtt ggtgttcgtc
    32341 gcagcaagcc atccaggccg gataaggcgt tcacgccgca tccggcaatc gtgcacaatg
    32401 cctgatgcga cgct
//
LOCUS       DAEVZA010000041        32373 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEVZA010000041 DAEVZA010000000
VERSION     DAEVZA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.041"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(31..596)
                     /locus_tag="LAF92_002019"
                     /pseudo
     CDS             complement(31..596)
                     /locus_tag="LAF92_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1176..1469
                     /gene="iss"
                     /locus_tag="LAF92_002020"
     CDS             1176..1469
                     /gene="iss"
                     /locus_tag="LAF92_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HBI9791035.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITFGIYTPLEARVYCSQ"
     gene            complement(1527..1702)
                     /locus_tag="LAF92_002021"
                     /pseudo
     CDS             complement(1527..1702)
                     /locus_tag="LAF92_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2280..3379
                     /locus_tag="LAF92_002022"
                     /pseudo
     CDS             2280..3379
                     /locus_tag="LAF92_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4615..5730
                     /gene="iroB"
                     /locus_tag="LAF92_002023"
     CDS             4615..5730
                     /gene="iroB"
                     /locus_tag="LAF92_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HBI9791036.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIKGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPERKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGQGADRPVNARVVAERGCGIIPGDVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
     gene            5870..9529
                     /gene="iroC"
                     /locus_tag="LAF92_002024"
     CDS             5870..9529
                     /gene="iroC"
                     /locus_tag="LAF92_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HBI9791037.1"
                     /translation="MPANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAAL
                     TPLLTRQAVNDALAGNPARLPWLACGLLLIAFFDFIGNYVRRGYAGMLSLWVQHTLRG
                     RVFDSIQKLDGAGQDALRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVML
                     WMSPAMTLIVVCVLVCLAITALRARRRVFAQTGLASDQLANLTEHIREVLAQISVVKS
                     CVAEMRETHWLDRQSRQIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHG
                     RIDLGTFVAFASFLAMLTGPTRVLASFLVIAQRTQASVERVFALIDTRSQMEDGTESI
                     NSQVVGLELENMSFDYHHGDRHILSDISFSLRAGETVAVVGASGSGKSTLLMLLARFY
                     DPCSGKIWLNTSEGRQNLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATAD
                     DIRRAAAAAGASDFINALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDD
                     TTSAVDAVTEAEINTALGRYADEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPA
                     ELEARCPAFRALMTGDSDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPE
                     NAVQQALAGKGRKVTSLLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGD
                     MSTIGLCALLALSLVIVGWCSYSLQTVFAARAAESVQHSVRLRSFGHMLRLGLPWHEK
                     HADSRLTRMTVDVDSLARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPV
                     AALATMIYRRLSTPAYAQARLEIGKVNSTLQEKVSGMRVVQSHGQQELEGARLRALSE
                     RFRATRVRAQKYLAVYFPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQ
                     FYGPVQQLSGIVDAWQQATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSY
                     PDSHEPALNKLTLTIPEGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDD
                     ASLAEYRRQIGLVDQDVALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQ
                     GFRTPVNNGGADLSAGQRQLIALSRAQLANAHILLLDEATSCLDRTSEERLMSSLTDV
                     VHAGKHSALIVAHRLTTAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSS
                     TALHRQHNMKEETPG"
     gene            9633..10862
                     /gene="iroD"
                     /locus_tag="LAF92_002025"
     CDS             9633..10862
                     /gene="iroD"
                     /locus_tag="LAF92_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HBI9791038.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFSTLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQIRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNGRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            10947..11903
                     /gene="iroE"
                     /locus_tag="LAF92_002026"
     CDS             10947..11903
                     /gene="iroE"
                     /locus_tag="LAF92_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HBI9791039.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSTTSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     VVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(11948..14125)
                     /gene="iroN"
                     /locus_tag="LAF92_002027"
     CDS             complement(11948..14125)
                     /gene="iroN"
                     /locus_tag="LAF92_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HBI9791040.1"
                     /translation="MRINKILWSLTVLLVGLNSQVSVAKSSDDDNDETLVVEATAEQV
                     LKQQPGVSVITSEDIKKTPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGP
                     ENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGA
                     AGGVVNIITKRPTNDWHGSLSLYTNQPESSDEGATRRANFSLSGPLAGNALTTRLYGN
                     LNKTDADSWDINSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIY
                     AGDTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRM
                     NEGLSGGGEGRILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDP
                     SSTSLTVNDSDISGISGSAADRSSKNHSQISALYIEDNIEPVPGTNIIPGLRFDYLSD
                     SGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGG
                     CYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAY
                     ILKWQNGGKALVDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTGNPLSVIPKYTIN
                     NSLNWTITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDI
                     NKNLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
     gene            complement(14991..16025)
                     /locus_tag="LAF92_002028"
     CDS             complement(14991..16025)
                     /locus_tag="LAF92_002028"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBI9791041.1"
                     /translation="MQSASKLIYRGKLLGSLPTVGEIHKEIAVSEETVTWISLQRDII
                     ANILLGKDPRLLVIVGPCSIHDVQAAVDYAKRLFVLQNKYLSKMYIVMRTYFEKPRTR
                     KGWKGIMHDPDLNGSYDVEKGIRYARQCLSSITTMRVATATEFLDPFLTPYIADLICW
                     GAIGARTTESQTHRQLASGLHCPVGFKNSTDGNINLAIDAIIAAREQHIVYMTSLTNS
                     ISTLLTDGNPHGHLILRGGREPNYGLSDITKAVKLMHDEGINHRLIIDCSHGNSGKVA
                     KRQISVARQVIDNRKKIPGYVAGIMVESFLQDGKQSDSFPLEYGQSVTDECISWQQTE
                     QLLSTLAAQL"
     gene            complement(16585..16893)
                     /locus_tag="LAF92_002029"
     CDS             complement(16585..16893)
                     /locus_tag="LAF92_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791042.1"
                     /translation="MDSKKMWRSNYAPPLLRILWRLGIRLPPLPFMPFWQVTLLMGGL
                     WGISWGCAMWFMYWGPSGMVAGEAIIISITSGFLFGLLMASFHWWRRKVNRLPPWNDV
                     "
     gene            complement(16992..17174)
                     /locus_tag="LAF92_002030"
     CDS             complement(16992..17174)
                     /locus_tag="LAF92_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBI9791043.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLF
                     SLSFISVQVHDNIITV"
     gene            complement(17171..17368)
                     /locus_tag="LAF92_002031"
     CDS             complement(17171..17368)
                     /locus_tag="LAF92_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HBI9791044.1"
                     /translation="MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPH
                     DMVESSAAAWRDEMSRKVAGK"
     gene            18050..19324
                     /locus_tag="LAF92_002032"
     CDS             18050..19324
                     /locus_tag="LAF92_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI9791045.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RNGIVTDNHRRDIENQLVRVNNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRYYQSKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRLELQKELVNTDVEGEIIIRALSDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPSEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGASVPWYKVIATPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPIND"
     gene            19299..21413
                     /locus_tag="LAF92_002033"
     CDS             19299..21413
                     /locus_tag="LAF92_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HBI9791046.1"
                     /translation="MQQDRSMTNRNFRQIINLLDLRWQRRVPVIHQTETAECGLACLA
                     MICGHFGKNIDLIYLRRKFNLSARGATLAGINGIAEQLGMATRALSLELDELRVLKTP
                     CILHWDFSHFVVLVSVKRNRYVLHDPARGIRYISREEMSRYFTGVALEVWPGSEFQSE
                     TLQTRISLRSLINSIYGIKRTLAKIFCLSVVIEAINLLMPVGTQLVMDHAIPAGDRGL
                     LTLISAALMFFILLKAATSTLRAWSSLVMSTLINVQWQSGLFDHLLRLPLAFFERRKL
                     GDIQSRFDSLDTLRATFTTSVIGFIMDSIMVVGVCVMMLLYGGYLTWIVLCFTTIYIF
                     IRLVTYGNYRQISEECLVREARAASYFMETLYGIATVKIQGMVGIRGAHWLNMKIDAI
                     NSGIKLTRMDLLFGGINTFVTACDQIVILWLGAGLVIDNQMTIGMFVAFSSFRGQFSE
                     RVASLTSFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMGPISLETNGLSYRYD
                     SQSAPIFSALSLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGRVLINGIDIRQI
                     GINNYHRMIACVMQDDRLFSGSIRENICGFAEEMDEEWMVECARASHIHDVIMNMPMG
                     YETLIGELGEGLSGGQKQRIFIARALYRKPGILFMDEATSALDSESEHFVNVAIKNMN
                     ITRVIIAHRETTLRTVDRVISI"
     gene            complement(21583..21894)
                     /gene="cvaC"
                     /locus_tag="LAF92_002034"
     CDS             complement(21583..21894)
                     /gene="cvaC"
                     /locus_tag="LAF92_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V"
                     /protein_id="HBI9791047.1"
                     /translation="MRTLTLNELDSVSGGASGRDIAMAIGTLSGQFVAGGIGAAAGGV
                     AGGAIYDYASTHKPNPAMSPSGLGGTIKQKPEGIPSEAWNYAAGRLCNWSPNNLSDVC
                     L"
     gene            complement(21872..22108)
                     /gene="cvi"
                     /locus_tag="LAF92_002035"
     CDS             complement(21872..22108)
                     /gene="cvi"
                     /locus_tag="LAF92_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HBI9791048.1"
                     /translation="MDRKRTKLELLFAFIINATAIYIALAIYDCVFRGKDFLSMHTFC
                     FSALMSAICYFVGDNYYSISDKIKRRSYENSDSK"
     gene            22254..23031
                     /locus_tag="LAF92_002036"
                     /pseudo
     CDS             22254..23031
                     /locus_tag="LAF92_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            23033..23302
                     /locus_tag="LAF92_002037"
     CDS             23033..23302
                     /locus_tag="LAF92_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HBI9791049.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            23299..24279
                     /locus_tag="LAF92_002038"
     CDS             23299..24279
                     /locus_tag="LAF92_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HBI9791050.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGEIWNPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDEFLGVEQVFRYLSAVAGEDALRESIRLNTEVV
                     SLRKDAGHRVIRCASEGKDTEDIFDRVIIATGELWQPRRPPLPGEENFSGTLITSRDY
                     QEPEAFKGKNILIIGGGVSGADIASDLVPFARSVSLSVKKMGLYLPRQFPTGPNDMMH
                     SYLGRCLLSQMNYEDFIGYLDTMMPDYMQAYRASSLLPDMANNNAVHVNEKIIPNVAA
                     GLIKVKPQAERFTGEGAIKFVDASQEKYDVIITCTGYEMPDYSFIRKRTA"
     gene            24355..24735
                     /locus_tag="LAF92_002039"
     CDS             24355..24735
                     /locus_tag="LAF92_002039"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBI9791051.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            24732..25079
                     /gene="tnpB"
                     /locus_tag="LAF92_002040"
     CDS             24732..25079
                     /gene="tnpB"
                     /locus_tag="LAF92_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI9791052.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            25129..26666
                     /locus_tag="LAF92_002041"
                     /pseudo
     CDS             25129..26666
                     /locus_tag="LAF92_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
     gene            26706..26972
                     /locus_tag="LAF92_002042"
                     /pseudo
     CDS             26706..26972
                     /locus_tag="LAF92_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
     gene            complement(26959..27411)
                     /gene="def"
                     /locus_tag="LAF92_002043"
     CDS             complement(26959..27411)
                     /gene="def"
                     /locus_tag="LAF92_002043"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBI9791053.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEKG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIGLRLGNGKYCTLRLFFNQV"
     gene            complement(27520..31653)
                     /gene="hbp"
                     /locus_tag="LAF92_002044"
     CDS             complement(27520..31653)
                     /gene="hbp"
                     /locus_tag="LAF92_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021534952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-binding protease autotransporter Hbp"
                     /protein_id="HBI9791054.1"
                     /translation="MNRIYSLRYSAVARGFIAVSEFARKCVHKSVRRLCFPVLLLIPV
                     LFSAGSLAGTVNNELGYQLFRDFAENKGMFRPGATNIAIYNKQGEFVGTLDKAAMPDF
                     SAVDSEIGVATLINPQYIASVKHNGGYTNVSFGDGENRYNIVDRNNAPSLDFHAPRLD
                     KLVTEVAPTAVTAQGAVAGAYLDKERYPVFYRLGSGTQYIKDSNGQLTQMGGAYSWLT
                     GGTVGSLSSYQNGEMISTSSGLVFDYKLNGAMPIYGEAGDSGSPLFAFDTVQNKWVLV
                     GVLTAGNGAGGRGNNWAVIPLDFIGQKFNEDNDAPVTFRTSEGGALEWSFNSSTGAGA
                     LTQGTTTYAMHGQQGNDLNAGKNLIFQGQNGQINLKDSVSQGAGSLTFRDNYTVTTSN
                     GSTWTGAGIVVDNGVSVNWQVNGVKGDNLHKIGEGTLTVQGTGINEGGLKVGDGKVVL
                     NQQTDNKGQVQAFSSVNIASGRPTVVLTDERQVNPDTVSWGYRGGTLDVNGNSLTFHQ
                     LKAADYGAVLANNVDKRATITLDYALRADKVALNGWSESGKGTAGNLYKYNNPYTNTT
                     DYFILKQSTYGYFPTDQSSNATWEFVGHSQGDAQKLVADRFNTAGYLFHGQLKGNLNV
                     DNRLPEGVTGALVMDGAADISGTFTQENGRLTLQGHPVIHAYNTQSVADKLAASGDHS
                     VLTQPTSFSQEDWENRSFTFDRLSLKNTDFGLGRNATLNTTIQADNSSVTLGDSRVFI
                     DKNDGQGTAFTLEEGTSVATKDADKSVFNGTVNLDNQSVLNINDIFNGGIQANNSTVN
                     ISSDSAVLGNSTLTSTALNLNKGANALASQSFVSDGPVNISDAALSLNSRPDEVSHTL
                     LPVYDYAGSWNLKGDDARLNVGPYSMLSGNINVQDKGTVTLGGEGELSPDLTLQNQML
                     YSLFNGYRNIWSGSLNAPDATVSMTDTQWSMNGNSTAGNMKLNRTIVGFNGGTSPFTT
                     LTTDNLDAVQSAFVMRTDLNKADKLVINKSATGHDNSIWVNFLKKPSNKDTLDIPLVS
                     APEATADNLFRASTRVVGFSDVTPILSVRKEDGKKEWVLDGYQVARNDGQGKAAATFM
                     HISYNNFITEVNNLNKRMGDLRDINGEAGTWVRLLNGSGSADGGFTDHYTLLQMGADR
                     KHELGSMDLFTGVMATYTDTDASADLYSGKTKSWGGGFYASGLFRSGAYFDVIAKYIH
                     NENKYDLNFAGAGKQNFRSHSLYAGAEVGYRYHLTDTTFVEPQAELVWGRLQGQTFNW
                     NDSGMDVSMRRNSVNPLVGRTGVVSGKTFSGKDWSLTARAGLHYEFDLTDSADVHLKD
                     AAGEHQINGRKDSRMLYGVGLNARFGDNTRLGLEVERSAFGKYNTDDAINANIRYSF"
     gene            31824..32015
                     /locus_tag="LAF92_002045"
                     /pseudo
     CDS             31824..32015
                     /locus_tag="LAF92_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(31983..32198)
                     /locus_tag="LAF92_002046"
                     /pseudo
     CDS             complement(31983..32198)
                     /locus_tag="LAF92_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630380.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         8221 a         8046 c         7809 g         8297 t
ORIGIN      
        1 agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcactccga taacatggtg
       61 tatacggaga agcacatccg tatcatcagt gtgacgactg cggcggccat ccatccagtc
      121 atcggttcgt ctgagaatga cgtgcaactg cgcacgcgac acccggagac aacggctgac
      181 taagcttact ccccatcccc gggcaataag ggcgcgtgcg ctatccactt ttttgcccgt
      241 ccatattcaa cggcttcttt gaggagttca ttttccatcg ttttcttgcc gagcaggcgc
      301 tggagttctt taatctgctt catggcggca gcaagttcag aggcaggaac aacctgttct
      361 ccggcggcga cagcagtaag acttccttcc tggtattgct tacgccagag aaataactgg
      421 ctggctgcta caccatgttg ccgggcaacg agggagaccg tcatccccgg ttcaaagctc
      481 tgctgaacaa ttgcgatctt ttcctgtgtg gtacgccgtc tgcgtttctc cggccctaag
      541 acatcaatca tctgttctcc aatgactagt ctaaaaacta gtattaagac tatcacttat
      601 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagtgcacga atcctagagg
      661 cagaatgttt ctccacatgc ttgtggagaa accgccgcat tttgcccgtt ctgtacgata
      721 ctccgtagcc agagataaga tattctgttg ctgtatgcta catacattac actatacgaa
      781 atcaggtctg ttcaatcagt aaggaaataa acggacaaac gttatgtaac ccagacagag
      841 atatattgtc tgggaaaata gtggcttttg tggcgaaaac tagtaaaaca gcaacccgaa
      901 ccacttgatg tgcatcgttt ttgattattc ccgtatactc ttgcagaagg agttctccgt
      961 cgggctactg tcatggttaa tgcggggaat atggcgacaa tacaacacac ctaaaagagt
     1021 aatggacaga tgaagcggtt tattcatttc ccatgattct gagtacctac caagtctgag
     1081 taaccacttt tatactttta attttcgttc atttagctat cgtttaatta ttatcacata
     1141 ggattctgcc gtttttaaca atgcaggata ataagatgaa aaaaatgtta ttttctgccg
     1201 ctctggcaat gcttattaca ggatgtgctc aacaaacgtt tactgttgga aacaaaccga
     1261 cagcagtaac accaaaggaa accatcactc atcatttctt cgtttcggga attggacaag
     1321 agaaaactgt tgatgcagcc aaaatttgtg gcggtgcaga aaatgttgtt aaaacagaaa
     1381 ctcagcaaac attcgtaaat ggattgctcg gttttatcac ttttggcatc tatactccgc
     1441 tggaagcccg ggtatattgc tcacaatagt tgcccatcga tatggggagc tcatctgcac
     1501 tgttcattaa tatacttctg ggctccctac agttgttttt gcatagtgat aagcctctct
     1561 ctgagggagg aaataatcct gttcagcgat gtctgccagt cgggggggct gcattatcca
     1621 cgcccgaggc ggtggtggct tcacgcgggg atgggcagat tgatctgata tgcaaccgac
     1681 gacgaccagc ggcaacatca tcacgcagag cttcattttc agatttgggc caccttttga
     1741 tttctcgttt aacttttgca gtcaccagac tgtgatttat tttaacaaat caatagcttt
     1801 cataaaattc tcgaagctga aagcttaact gaaccaacca gcctggctgg tgttttctaa
     1861 agacaaaaag gccatcagca aaacactgat agtctgaatc acctttatca atcatgtatg
     1921 aagaaaatat gcgcaattag ttgacagtga ttatcatttt cattaaaaag agcgcgtagc
     1981 attcttattt catgaggaaa tttacccgcc aacaacctga gtagcgaaag ctgttcatcc
     2041 ccaatatttt tgctggcggg ttcttttttc tactgtcctc ctccccagag gaagcattcc
     2101 cattaatcat tactggcgag gaggcaagag aaaaagtcgg ttctttgtcc ctaaataaca
     2161 gcatcatttc agtccatgca aaatctgatt taccagctca cataataacc tgatgataca
     2221 cagtgtaagt tcacagagat attgcaattg cctccggata agtaagggga gattgcacta
     2281 tgcaaatgca gcacctgagg ttggctatcc taagcagccg attatgcgtt gaggctttca
     2341 gtgatggctg atatagcaac aatgagaatg aaggctctgc acttctggga taaacacggt
     2401 atttctgcag cttctgaagc ctttggcgta tcctgccgca cgctttactg gtggcgtcag
     2461 ttactgaaca agggaggacc tgaggggcta atcccgcaca gtaaggcacc tctggtgcga
     2521 cgaaaaaagc actggcatcc cgatgtgctg aaagagattc gacgcctcag gacagagctg
     2581 ccgaacctcg gtaaagagca gatttttgtt cgcctgaagc cctggtgcgc ataacgccat
     2641 cagacctgcc cgagtgtttc caccatcggc agaatgatcg ccaccgcaca cgataaaatg
     2701 cggatgatac cagtacgtct gagctcgcgg ggtaaggctc tgcttgtcaa aaagcgaacc
     2761 accaaacccc cgcaggccaa agcactaccg tccggtaaag acaggagagc tcattgggat
     2821 ggacgccatt gagctcagaa tgggtgaaat gcgtcgctat atcattacca tgatcgacga
     2881 acacagcgat tacgtactgg cgctggctgt gccgtcgctc aacagtgata tcgtcaatca
     2941 ctttttcagc cgtgcagccc ggctgttccc tgtcggtatc agccagatca tcacagataa
     3001 cgggaaagag ttcctgggaa gctttgacaa aacgctgcag gaagccgcca tcaaacacct
     3061 ctggacctat ccctacacgc caaaaatgaa cgctatctgt gaacgtttta accgggcatt
     3121 aagagagcaa ttcattgaat ttaatgaaat tttactcttt gaggatctgg cgttgtttaa
     3181 cctgaaactg gcggaatatc tggcgctgta taacagcaaa agactccaca aggcgctcgc
     3241 actaacgacg cccgtggaat atattttaaa agagaacaaa aattgcaata tgtggtggac
     3301 ccatacacag caaacgcaca ggccgccccg gttgggtaga tatgtgggat ggctggttca
     3361 gacttcagga tatggttgag gctggtgatg aagtctcttt atcaggagat ctgatcaaga
     3421 gacagccgcg cagggggatt cactatgctg gattctttta acaaattctc cgctaaccaa
     3481 aaaatagact ttttgcattc gtaaatgcgt taagagataa tttttaaagt tccatcaatc
     3541 tcccggccag ttcgtctcgc ggcattactc actatccgca caacttcagt aagatcatga
     3601 ctttttcaat cgttatctgt tgaccaagct ttgaaaccac tagcgtcccg tcaacctgac
     3661 ctggaatcgt cgagaaccgc ccatagttgt gaattggcta gagtgtgatg gaaagcaacg
     3721 ctaaacgacc tagtcacaca ttctcttttc cctaacaatg accatttagt gaaataacgt
     3781 atccaaaatg atgacgtgaa agcctgacac tggcagcgaa aagccgaaaa aactcacctg
     3841 gtcaggagca aaaatcggat tattcgccaa cagcgtaata tcagcagtag ttctcgacac
     3901 gcttgcgcta tagacagagg atgaaacgct gctggtcgga gttctctata catttttggc
     3961 gacggcagaa ccgctcacca atgccttaac gtctatcagc cttccggtga gttggaacag
     4021 catgctcagc gtttgcaaga tattctccac ggtctgtagc agcgtaggtt tcaatttaaa
     4081 tatccccaga tagccctctg ggagcttccc tttttattaa gttttaatta ccgttgcaac
     4141 agttaaatga tattggtaat tattatcatt agcatttgtg tctgtgttgt ttctggtaag
     4201 gctgaaaggg aacaaacatc gcctgtacaa cggcgttttt acttacagac cagcgaaggc
     4261 gagactatca ggatgatgca tcgtgtgtct aagaaatcac ggttctttcc agattcgttg
     4321 ttcggtctaa cactgcacaa atgcgtcaga acatcccggc gtagctgtcc gacgccattt
     4381 gggctgttca acaggacaat ggcgcgtaag cgttaatgga agtggggata cggagttccc
     4441 tgttcacctg tgtatgtcac ggaaaagtct ctgactgagc tgtccataac gctggtgaag
     4501 cacaaatggc gcagcatctg gtaaagcagg tttaacgaaa agtcgtgtcc tgctgctggc
     4561 atatgagtgc gtcgactgcc tgatttagat cgtcaagcgg agagggattt tctcatgcgt
     4621 attttgtttg taggcccgcc tctctatggg ctgttgtatc ctgtgctgtc tctggcgcag
     4681 gcatttcgcg ttaacggtca tgaagtgctg atcgccagtg gaggacaatt cgcacaaaaa
     4741 gcggcagagg ccgggttggt ggtgtttgac gccgcacccg gtctggattc cgaagctggt
     4801 taccgccacc atgaggcaca gcggaaaaaa agtaatatcg gaacccaaat gggcaacttc
     4861 tcattcttca gcgaagaaat ggccgaccat ctggtggagt ttgccggaca ctggcggcct
     4921 gacctcatca tctaccctcc gctcggagtc atcgggccgc tgattgccgc caaatatgat
     4981 ataccggtcg tgatgcaaac cgtaggcttc ggccataccc cctggcatat caagggtgta
     5041 acgcgttcgc tcacggacgc ctatcgtcgc cacaatgtgg gggccacacc acgtgatatg
     5101 gcctggatcg acgtaacacc gccgagtatg agcattctgg aaaacgacgg tgaacccatt
     5161 attccgatgc agtacgtccc gtataacggt ggcgcggtgt gggaaccatg gtgggagaga
     5221 aggcccgagc gtaaacgtct gctggtgagt ctcggcactg tcaaaccgat ggttgacggc
     5281 cttgatctaa tcgcctgggt tatggattct gccagtgagg tcgatgccga gatcatcctg
     5341 catatttcag ccaatgcgcg gtccgatttg cgctcgctgc cgtcaaatgt tcgcctggtt
     5401 gactggatac ccatgggtgt cttcctcaac ggcgcagatg gtttcattca tcatggcgga
     5461 gcaggcaata ccctgacagc gctacacgct ggtattccac aaatcgtctt cggccaggga
     5521 gccgatcgcc cggtaaatgc ccgcgttgtt gccgaacgcg gctgcgggat tatccctggc
     5581 gacgtcggcc tgtcgagtaa catgatcaat gctttcctca acaatcgctc acttcgcaaa
     5641 gcctctgaag aggtagcagc ggaaatggcg gcgcaaccct gcccaggtga agtggcgaaa
     5701 agcctgatca caatggtaca gaaagggtaa ccactacacc ttcatgataa tcatatgcca
     5761 gcacccatcc aggagtcatg catccataac aatgcgggac cggagcgtgc tggctttttt
     5821 gcggaacgat ccggccagtg gatcgtattt tacagacgga ggaggcttaa tgcctgcgaa
     5881 tcacactccc acaccggctc agtcatggat agttcgccta gcgcgcgtgt gctgggaacg
     5941 taagaaactt agtgtcatcg tggtggtagc gtcagtatcg actattttgc tggctgcgct
     6001 gacgccactg ctgacaagac aggctgtgaa tgacgcactg gcgggcaatc cggcccgcct
     6061 gccgtggctg gcctgcgggt tactgttgat cgcttttttt gatttcatcg gtaactatgt
     6121 gcgccgtggt tatgccggga tgctctcact ctgggtgcag cataccctca gaggacgggt
     6181 attcgacagt attcagaaac ttgacggcgc aggccaggat gcgctgcgca ccgggcaggt
     6241 gatttcacgg accaacagcg atctgcagca ggtgcatacc ctgctgcaga tgtgcccggt
     6301 gccgctggca gtgttcactt attacattgc cggcattgcc gtgatgctgt ggatgtcccc
     6361 tgccatgacg cttatcgtcg tgtgcgtact ggtatgcctg gcgatcaccg cgcttcgtgc
     6421 gcgtcgtagg gtcttcgcgc aaaccgggct ggcctcggac caactggcga atctcaccga
     6481 acatatacgc gaggtgctgg cacagatctc agtggtaaaa tcctgtgtgg cagagatgcg
     6541 tgaaacgcac tggctcgata ggcagtcgcg gcagattgtg cgtgtacgca tcggtgcggt
     6601 tatctcgcag gcgatgcctg gggccaccat gctggcgcta ccggtgctcg ggcaaatcgt
     6661 cctgctgtgc tacggcgggt ggtcggtcat gcacgggcgg atcgatctcg gtaccttcgt
     6721 tgcattcgcc agcttcctcg cgatgctgac cgggccaacc cgcgtactgg catcgtttct
     6781 ggttatcgca cagcgcactc aggcgtccgt ggagcgggtg tttgcactga tcgacacccg
     6841 ttcacagatg gaggacggga cggagtcgat taacagtcag gttgtcggac tggaactgga
     6901 gaatatgagc tttgactacc accatggcga cagacatatc ctcagcgata tctccttttc
     6961 cctgcgcgcc ggtgaaaccg tggcggtggt gggcgcatcg ggttcaggaa aatcgaccct
     7021 gttgatgctg ctggcgcgtt tttatgatcc ctgctccgga aagatatggc tcaacaccag
     7081 cgaaggccga caaaatcttc gcgatatcag actggaggcg cttcgtcgcc gggtaggcat
     7141 cgtatttgaa gatgcttttc tgtttgccgg tacggtggcg gaaaatatcg cctatggcca
     7201 ccctcaggca acggcggacg acattcgccg tgcggcagct gctgcaggag ccagcgattt
     7261 tatcaacgcc ctgccgaaag gcttcgatag cctgttaacc gaacggggta cgaatctttc
     7321 cggcgggcag aggcagcgaa tagcgctggc gcgggcgctc attactgcac cggacgtgtt
     7381 aatcctggat gataccacct cagcggttga tgctgttacg gaagcggaga ttaataccgc
     7441 gctgggtcgc tatgctgacg aagggcatat gctgctggtg attgcccgac ggcgttcaac
     7501 acttcagcta gccagccggg ttgtggtgct ggataagggc cgtatggtgg ataccggaac
     7561 cccggcagaa cttgaagcgc gctgtccggc gttccgcgca ctgatgaccg gcgacagcga
     7621 ttttctggcc acgtcccaca atagccacaa cgaattgtgg ccggctgaac cagcgacaca
     7681 agacgatgta acggatacgg gggataaagg ttttgtcgcc cgtatgaccc gcgtaccgga
     7741 aaatgcagta cagcaggcgc tggccggtaa aggccgcaaa gtcacgtcac tactgaagcc
     7801 tgtggcgtgg atgttcgtca tcgccgctct gctgatcgca ctcgattctg cggcaggcgt
     7861 aggggtgctg atactgttgc agcacggcat tgactccggt gtcgccgcag gcgatatgtc
     7921 gaccatcggc ctctgtgccc tgctcgccct gagcctggtc attgtgggct ggtgcagtta
     7981 ttctctgcag acggtcttcg ccgccagagc ggcggaatca gttcagcatt cggtgcgctt
     8041 gcgcagcttc ggccatatgc tgcgtcttgg actcccctgg catgaaaagc atgccgattc
     8101 gcgtcttacc cgcatgaccg ttgatgtgga ctctctcgcc cgctttctgc aaaacggcct
     8161 tgccggtgcg gccaccagtc tggtgacgat gttcgcaatc gccgccacca tgttctggct
     8221 cgacccgttc ctggcgctga cggcattaag cgcagtgcca gtggccgcac tggcaaccat
     8281 gatttatcgc cgcctcagta cccctgctta tgcacaggca cggctggaaa taggcaaagt
     8341 caacagcacc ctgcaggaaa aagtctctgg catgcgtgtc gtgcaatcgc atggtcagca
     8401 ggaactggag ggcgcccggc tgcgcgcgtt atcggagcgt ttccgcgcaa cccgtgtgcg
     8461 agcacaaaaa taccttgcag tctattttcc gttcctgaca ttctgcaccg aggcctccta
     8521 tgccgctgtt ctgttagtgg gagcttcgca ggtcgccgct ggagaaatga ctgccggggt
     8581 actggcggct ttcttcctgt tgctggggca attctatggg ccagtgcagc agttatcagg
     8641 gattgtcgac gcctggcagc aggcgacagc cagcggcaaa catattgatg aactactggc
     8701 gacagaaggc actgagaacc tcggctcctc ttcggtcctc cctgtcaccg gtgcactgca
     8761 tcttgatgag gtcacgttca gttatcccga cagtcacgag ccagctctga acaaacttac
     8821 cctgacgatc ccagagggaa tggttgttgc ggtcgtcggt cgcagcggtg cgggtaagtc
     8881 gacgctgatt aagctgattg ccgggttgta tttccccacg cacggcaaca tcagaatcgg
     8941 tgtgcaaatg ctcgatgatg cctcgctcgc tgagtatcgt cgccagattg ggcttgtcga
     9001 tcaggatgta gcactgttta gtagtgatat tgcagaaaac attcgttatt cacggccatc
     9061 cgccaccaat gaagacgttg aaattgcctc acagcgggca gggctgtatg agatggtgtg
     9121 caatctgccg cagggattcc ggacaccagt gaataacggc ggagccgatc tgtccgcagg
     9181 tcagcgccag ttgattgcgc tgtcccgcgc gcaactggct aatgctcaca tcctgctgct
     9241 cgacgaagcc acgtcatgtc tggatcgcac atccgaagaa cgactgatgt catcgttaac
     9301 agatgtcgtg catgccggga agcactcggc gctgattgtt gcacatcgtc tgaccaccgc
     9361 gcaacgctgc gatctgattg ccgttattga taaggggtta cttgcggaat acggaaccca
     9421 cgaacagctg ttatctgcgg gcggcctcta tacccgctta tggcatgaca gcgtcagcag
     9481 tactgcgctc catcgccagc acaacatgaa ggaggaaacc ccgggatagt tactggacac
     9541 gtaatgtatt aaaaacacag tcagaagcgg cggtaccgtg aatagccgct ttaattattt
     9601 atactgacat ccttaatttt taaagagtat gaatgctgaa catgcaacaa catccctctg
     9661 ctatcgccag cctgcgcaac caactggcag cgggccacat tgctaacctt actgacttct
     9721 ggcgcgaagc tgagtcgctg aatgttcctc ttgtgacgcc agtcgaagga gcggaagatg
     9781 agcgagaagt tacctttctg tggcgcgccc gacatcctct gcagggcgtt tatctgcgtc
     9841 tgaaccgggt gacggataaa gagcacgtag aaaaaggaat gatgagcgcc cttcccgaaa
     9901 cggatatctg gacactgaca ctgcgtttac ccgcaagtta ctgcggctcc tattcgctgc
     9961 tggaaatccc ccccggcact acggctgaga cgattgcact gtccggaggc cgtttttcca
    10021 ccctcgccgg aaaagccgat ccgctaaaca aaatgccgga gatcaacgtt cgggggaacg
    10081 caaaggaatc agtgctgaca cttgataaag ctcccgccct gtcggaatgg aacgggggct
    10141 tccacaccgg acaactgctt acctccatgc gcattatcgc cgggaaatct cgccagattc
    10201 ggctctatat tccggatgtt gatatttctc agcccctcgg gctggtcgtg ctgcccgatg
    10261 gtgaaacctg gtttgatcac cttggcgtat gcgcggcaat tgacgccgcc ataaataacg
    10321 ggcgcatcgt gcccgtggct gtactgggca ttgacaacat taatgaacat gaacgcactg
    10381 agatactcgg cgggcgcagc aaactgataa aggatatcgc cggacatctg ctgccgatga
    10441 ttcgcgctga acaaccgcag cgtcagtggg cagaccgttc gcgcacagtg ctggccgggc
    10501 agagcctcgg cgggatcagt gcgctaatgg gggctcgtta cgcaccggaa acgttcggtc
    10561 tggtgctcag ccactctcct tcaatgtggt ggacgccaga aagaaccagt cgaccaggct
    10621 tgttcagcga aaccgatacc tcatgggtga gtgagcatct gctttctgcc ccaccgcagg
    10681 gcgtacgtat cagcctgtgc gtgggatcgc tggaaggttc gacagtgcct cacgttcagc
    10741 agcttcacca gcggctgatt accgctggcg tcgaaagcca ttgcgcaatt tacaccggtg
    10801 gtcacgatta cgcatggtgg cgcggtgcac tgattgacgg gattggttta ctacagggtt
    10861 gagttgaccc acaaacactt tcaggaaacg gtacagactt cctgaataaa tcaaattgtc
    10921 acctgcggaa aaggaataat catcagatgt atgcccgcga gtatcgctca acacgcccgc
    10981 ataaagcgat tttctttcat ctttcttgcc tcacccttat ctgtagtgcg caagtttatg
    11041 cgaagccgga tatgcggcca ctggggccga atatagccga taaaggctcc gtgttttacc
    11101 atttcagcac cacctctttc gactctgtcg atggcacacg ccattatcgg gtatggacgg
    11161 ccgtgccgaa tacaaccgca ccggcatcgg gttacccgat tttatatatg cttgacggta
    11221 acgcagttat ggaccgcctg gatgacgaac tgctcaaaca attgtcagaa aaaacaccgc
    11281 cagtgatcgt ggctgtcggg tatcagacca acctcccttt cgatctcaac agcagggctt
    11341 acgactatac gccagcagca gaaagcagaa aaacagatct ccactcaggg cgttttagcc
    11401 gtaagagtgg tggcagcaac aacttccgcc agttactgga aacgcgtatt gccccaaaag
    11461 tggaacaggg actgaatatc gatcggcaac gccgcggctt atgggggcac tcctacggcg
    11521 gcctcttcgt gctggattcc tggctgtcct cctcttactt ccggtcgtac tacagtgcca
    11581 gcccgtcgtt gggcagaggt tatgatgctt tgctaagccg cgttacggcg gttgagcctc
    11641 tgcaattctg cgccaaacac ctggcgataa tggaaggctc ggcgacacag ggtgataacc
    11701 gggaaacgca tgctgtcggg gtgctgtcga aaattcatac caccctcact atactgaaag
    11761 ataaaggcgt caatgtcgta ttttgggatt tccccaactt gggacacggg ccgatgttca
    11821 atgcctcctt tcgccaggca ctgttagata tcagtggtga aaacgcaaat tacacagcag
    11881 gttgtcatga gttaagccac taaacactgc ccgcttttac gcgggcagta cgcctgaaac
    11941 actacgatca gaatgatgcg gtaactccgg catagtaagc ccggcctggc tcgttatagg
    12001 tattcgcccc ttcagaagat cggaagatct gtttattgag gatattactg acgccgacat
    12061 taagacgcag atttttatta atatcgtaat tgaagttcgt ccccaccagt gaataagcgc
    12121 ccagctcttt acctgacaga ccgccagtat cttcactgcg ggtttccgca tgagtacgcg
    12181 gtttttgtct gccatataac gtccagttga cgctggcaga aaacgcctgg gtgatggtcc
    12241 agttaagcga gttattgata gtatatttcg ggatgaccga cagaggatta ccggtgtctt
    12301 tttgctccga agtgatcatc catgtggcat tggtattcca gttcagacga tctttcacca
    12361 gtgggaaaga catactggct tcgataccgt ccaccagagc tttcccgcca ttctgccact
    12421 tgaggatata tgcgcctgaa gcggtttgcc cgataacgtt atccccggcc acgatcttat
    12481 tctggtaatc attgcggaag taggtcacac ttgcgtggta atcttcccag gtgaactcca
    12541 gcccaatttc tttattgacg ctgatttccg gatcgagatc tttattaccg atcaggtagc
    12601 acccgcctga tgtaatatct tttggacagc cattgccttt cgagtagagc agatagcctt
    12661 cactggattg atacaggttt ggggctttaa aggttcgggc aacccctgct ttgactttga
    12721 aataatcgcc caattcctgc gaaagattca gactggggct gaagttcccg ccggagtcgc
    12781 tgagataatc aaagcgcagg ccgggaatga tattcgtgcc aggaaccggc tcaatgttat
    12841 cttcaatata cagcgcactg atttgagaat gatttttact gctgcgatcc gcagcagagc
    12901 cagaaatacc gctgatatca ctgtcattca ccgtcaggct ggtagaggaa ggatcatcga
    12961 gcttatcgcg gttccactct gcaccaacgg tcagcgtttg atcaaccatc acattcaaag
    13021 gaatattaag ctcgccgctg gttcgccagg aactcaggcg attggtcgta aacttttcac
    13081 ccgctaaaat acgcccttca ccaccgccgg ataatccttc attcatgcgg gtattattgg
    13141 ttttctcgta ataaacacca aagcgacttt gtccccagtc ccagatacca ttatgcgtaa
    13201 tgccataatt ctgtcggtac aggcggttcg tctctttgcc ggattttgcc aggctttcgg
    13261 taactgcact ggaagaactg ttttgcgtat cgcccgcata gatattcccc tggcggctat
    13321 atccggcttc gaaatcgaga atctgctgcg gatttaattt ccacgagaca acgccgttaa
    13381 tatctttgtt acgtacccct tcatgcccgg ctgcgttttt cgtaccgacc ggagaattaa
    13441 tatcccaact gtcagcatcc gttttattca ggttaccata caaacgcgtg gtaagagcat
    13501 taccagccag aggcccacta aggctgaaat tggcgcgacg cgtagcgccc tcatcgctac
    13561 tttccggctg attggtgtat aacgacagcg aaccgtgcca gtcgttggtg ggacgtttgg
    13621 taatgatgtt caccaccccc ccggctgccc ccgaaccgta gcgcgccgcc gcagggccgc
    13681 ggatcacttc aatacgctca acctgttccg gtggcaccca gttggtgtca ccgcgggtat
    13741 cacgctctcc acgccagcta taacgcacgg agttacgtga cgtcaccggt acaccatcaa
    13801 ttaaaattaa ggtgttttcc ggccccatac cacgaatatc gatctggcgg ttgttaccgc
    13861 gtgtgcccga ggcgctattg ccggtaagat tgacgccagg cattttacga ataatatctg
    13921 aaaggtcgtt taccggaggg gtctttttaa tatcctcgct ggtaataacc gacacgcccg
    13981 gctgctgttt taatacctgc tcagcggtgg cttccaccac cagagtctcg tcattatcat
    14041 cgtcggagga tttggctact gatacctggc tattcaaccc aaccaggagc acagttagcg
    14101 accagaggat tttgttaatt ctcataccta ttccctaata aatgcctaac ttaaaatgtt
    14161 tgatcgttaa gctcacatcc ttgccagata tttcttactg ccattattgt ttttatataa
    14221 gaatgataat taatatcatt tagtaaaaga aaaagcaatc cctcacaaga taaatatatt
    14281 gatttttcat aaatatcaaa ttgatatata acatatgttt tttatttcat tgcacttgtc
    14341 gatcatcggg aattttgtca caatttctca cggatagtgt tcacattgtt tctgacctgc
    14401 atttcaggcg cgggcgctgc ttatgtatat aagggcctgt ggtcgacgaa tcagcataat
    14461 cagatcttcc tgcgaacaat ttctttcagc gtatcaggca gcactatcgg attatctacg
    14521 agcgtttttg attcacattg tgtgtgaaac ttcatacatc atgattcgct ttccctactc
    14581 agttctgatg attgtttttt attctattaa cacggagtta agattctacc ctcggtgtcc
    14641 tcattattaa tgaggaatgc tacctatcta aaaactgacc gaaactttgg ggcatccctg
    14701 gaggtttcca aggagaaaaa ggtgacagtg atagtgccac attgtgagga aattcctgag
    14761 aaactcctga gaaactcctg gttcacacac ataattcagg agcttgccat agatagtaac
    14821 caattgtttt atattttaag accattaaat acgcggctac gatagcgctt tatataatca
    14881 taaactgtag agataaaata tctgcttctc tgataacact aattgagtgt cttaaactaa
    14941 gtaatttcat ttttccccct ttaaaagcaa tatatgtgca atagttgaag ttaaagctgt
    15001 gctgccagag ttgataataa ttgttctgtc tgttgccatg atatgcattc atcagtaaca
    15061 gactgaccat actctagcgg aaaactatcg gattgttttc catcttgtaa aaagctttcc
    15121 accataatgc cagctacata tcctggtatt tttttccgat tatctataac ttgacgtgca
    15181 acggaaattt gccgtttagc cactttaccg ctattaccat ggctacaatc aattatcaga
    15241 cgatggttaa tgccctcatc atgcattaat ttcactgcct tagttatatc agacaaacca
    15301 tagttaggtt ctctacctcc acgtaatatt agatgtccat gtggattgcc atcggttaat
    15361 agtgtagata tactatttgt taaagatgtc atatatacaa tatgttgttc acgggctgca
    15421 ataatagcgt caatagctaa gttaatattt ccatcagtac tgtttttaaa tccaactgga
    15481 caatgtaaac cagacgcaag ttgacgatgc gtttgtgatt cagtggttct ggcaccaata
    15541 gcaccccaac atattaaatc agcaatatag ggagtaagaa aaggatcgag gaattctgta
    15601 gcggttgcga cacgcatcgt tgtgattgaa gacaaacact ggcgagcata tcgtatccct
    15661 ttctcaacat cataacttcc atttagatca ggatcgtgca ttattccctt ccagccttta
    15721 cgggttcgag gtttctcaaa ataagtgcgc attacaatgt acattttcga taagtactta
    15781 ttctgtaata cgaacaatcg cttcgcataa tcaactgcgg cctgaacgtc atggattgag
    15841 catggaccaa caatcacaag taagcgagga tcttttccca gcaaaatatt cgctataata
    15901 tctcgttgca gagagatcca tgttacggtt tcttccgata ctgcgatttc tttatgaatc
    15961 tcgcccacag tgggtaaact gccaagcaac ttcccgcggt agattaattt actagctgac
    16021 tgcatcttgt ctcctaaaag cgctatttca gaccatcagt tgcacacagt agatgataat
    16081 gacaatcatt tactgtataa ttggcaatat atggggattc atagtcattt ctgagataat
    16141 atacgttagg ccttctctcc cgtacggaaa tcacttgttt tccgtaaatt acctgaatct
    16201 taatccctga gctgcggcta gctgtggaac tcacaaaagc tgaccgggcc gtggcacagc
    16261 ctctctgctg gcagaacagc gtactgcaga agtcgtcgta ctgccgctgg tcccggcatc
    16321 aggtctgccc gcagatggca cttctgccac tgatctgccg gtggagctga acggcatgac
    16381 atatcaggcc tgccgtgggg attttgtggt gcgcctcgac ggcagcacct gtctgcagtt
    16441 atagaataaa gaaggcgggg aggtacgccg ggagggcgat ccgctggaag tggcgtagtg
    16501 gctgtaggcc tgtaatgatg caggaataga agttcgtgta caggttaatg aaagcatcac
    16561 cccgtaaaaa catgccggca cggtttacac atcattccat ggtggcagcc ggttaacttt
    16621 cctgcgccac cagtggaaag aggccatgag cagtccgaac aaaaaaccac tcgtgatact
    16681 tataataatg gcctcacctg ctaccattcc tgacggcccc cagtacataa accacattgc
    16741 acatccccag gaaatacccc ataagccccc catcagcagc gtaacctgcc agaacggcat
    16801 aaagggcaat ggcggaagcc ggatacccag ccgccagaga atacgcagca gaggaggagc
    16861 ataattactc cgccacatct ttttgctgtc catcagggca attgcccggg ctttttttgc
    16921 tcaaaagtca caggagcacc tccgttccat gatgtaaacg tgacacaact tattgttatt
    16981 gattattatt atcataccgt gattatgttg tcatgcacct gtacactgat aaaagaaagg
    17041 gagaacagga ggctggctga acaaccaatc cgttcgtcca gttcaatggc ggctatcagg
    17101 ttctgctccg caatgtactc acgaatgcgc ttcctgtcct ggctggccag catggtccag
    17161 aatatctcca tcatttaccg gcgactttcc tgctcatttc atcccgccat gcagcagcgg
    17221 aagattcgac catgtcatgg ggaagcacat cgccgcgctc gagttgctga cgtcctgcct
    17281 caacctggtc acggaaccag gtattatgct gccgttccac ggtctggcgc ataaaatccc
    17341 ggattaactg agagccatta cggtccatgc tttttgcagg gccataaaag catccttcag
    17401 ttcagcgtca attcttaaac tcatattaac ctgtgccatt tatcaccccg tcattaccac
    17461 gtatatacac agtatataac gattcagata tcgtcactag gatatgccgc gccagcggca
    17521 tggaaggcgg cactccgctg tttcatatga taccgccgaa gcccgatgta agccgctaca
    17581 gtcgtccgaa agtcaccagc ctcctccccc ctgccgtcat ccgtgcatca gctatgcact
    17641 gagtatgcct gcccttccct agagaatcct gccaggcttg ccacactgat atatcttgac
    17701 tttatgtaaa cgatatgaca ctttaacatg ataatgatta ccattctctt ttaatataca
    17761 gagaaactag gaaatagatg aatgagttat gttactttaa tattctctga caataaccta
    17821 aatcagttag attattgtca tttaataaat aatgacattc tttcatcata aataaaaaga
    17881 ctattgttta taatattgtt ctcagcatta tatgattatt tatcctgata actctcctat
    17941 gttgtatgtt tatatgattt tccttgaaac atataatgca aattttcgat ttattttcca
    18001 tcattaatcc agataaacaa caaactaata gtatgcaagg agacattatt tgtttcgcca
    18061 ggatgcttta gaaaacagaa aaatgaagtg gcagggacgg gcaatattac ttcccggaat
    18121 accactgtgg ttaatcatgc tgggaagcat tgtgtttatt acggcatttc tgatgttcat
    18181 tattgttggt acctatagcc gccgtgttaa tgtcagtggt gaggtcacaa cctggccaag
    18241 agctgtcaat atatattcag gtgtacaggg atttgttgtc aggcagtttg ttcatgaagg
    18301 gcagttgata aaaaaagggg atcctgttta tctgattgac atcagtaaaa gtacacgcaa
    18361 tggtattgtc actgataatc atcgccggga tatagaaaac cagctggttc gtgtgaacaa
    18421 cattatttcc cgtctggaag aaagtaaaaa aataacgcta gataccctgg aaaaacaacg
    18481 tctgcaatac acagatgcgt tccgtcgctc atcagacatt atacagcgtg cagaggaagg
    18541 gataaaaata atgaaaaata atatggagaa ttacagatac tatcagtcaa aaggactgat
    18601 taataaagat caattaacta accaagttgc attatattat caacaacaaa acaaccttct
    18661 cagtctgagc ggacaaaatg aacaaaatgc cctgcagata accactctgg agagtcagat
    18721 tcagactcag gcagcagatt ttgataatcg tatctatcag atggaactgc aacgactcga
    18781 attgcagaaa gaactggtta acactgatgt ggaaggcgaa atcattatcc gggcgttgtc
    18841 tgacgggaaa gttgactccc tgagtgtcac tgtagggcaa atggtcaata ccggagacag
    18901 ccttctgcag gttattcctg agaacattga aaactattat cttattctct gggtcccgaa
    18961 tgatgctgtt ccttatattt cggctggtga caaagtgaat attcgttatg aagccttccc
    19021 ctcagaaaaa tttgggcagt tctctgctac ggttaaaact atatccagga ctcctgcgtc
    19081 aacacaggaa atgttgacct ataagggcgc acctcaaaat acgccgggtg cctctgttcc
    19141 ctggtataaa gtcattgcga cgcctgaaaa gcagataatc aggtatgacg aaaaatacct
    19201 ccctctggaa aatggaatga aagccgaaag tacactattt ctggaaaaaa ggcgtattta
    19261 ccagtggatg ctttctcctt tctatgacat gaaacacagt gcaacaggac cgatcaatga
    19321 ctaacaggaa tttcagacaa attataaatc tgcttgattt gcgctggcaa cgtcgtgttc
    19381 cggttattca tcagacggag accgctgaat gtggactggc ctgcctagca atgatatgcg
    19441 gtcattttgg taagaatatt gacctgatat atcttcgccg gaagtttaat ctctctgccc
    19501 gtggagcaac ccttgcagga atcaatggaa tagcggagca actggggatg gccacccggg
    19561 ctctttcact ggagttggat gaacttcgag tcctcaaaac gccgtgtatt ctccactggg
    19621 atttcagtca cttcgtcgtt ctggtcagcg taaagcgtaa ccgttatgta ctgcatgatc
    19681 cggccagggg cataagatat atcagccggg aggaaatgag ccgatatttt acaggcgttg
    19741 cacttgaggt ctggcccgga agtgaattcc agtcggaaac cctgcagacc cgcataagtc
    19801 ttcgttcact gattaacagt atttacggta ttaaaagaac gctggcgaaa attttctgtc
    19861 tgtcagttgt aattgaagca atcaatctgc taatgccggt ggggacacag ctggttatgg
    19921 atcatgctat tcctgcgggg gacagagggc tactgacgct aatttctgct gctcttatgt
    19981 tttttatatt actcaaagct gcaacgagta cgctgcgcgc atggtcttca ctggttatga
    20041 gcacgctcat caatgtacag tggcagtcgg ggctgttcga tcatcttctc agactaccgc
    20101 tggcgttttt tgaacgccga aaattaggtg atatccagtc acgttttgac tcccttgaca
    20161 cattgagggc cacatttacc accagtgtga tcgggttcat aatggacagc attatggttg
    20221 tcggtgtttg tgtgatgatg ctgttatacg gaggatatct cacctggata gttctctgct
    20281 ttaccacaat ttacattttt attcgactgg tgacatacgg caattaccga cagatatcag
    20341 aagaatgtct tgtcagggag gcccgtgccg cctcctattt tatggaaaca ttatatggta
    20401 ttgccacggt aaaaatccag gggatggtcg gaattcgggg agcacactgg cttaatatga
    20461 aaatagatgc gataaattcg ggtattaagc taaccaggat ggatttgctc ttcggaggaa
    20521 taaatacctt tgttaccgcc tgtgatcaga ttgtaatttt atggctggga gcaggccttg
    20581 tgatcgataa tcagatgaca ataggaatgt ttgtagcgtt tagttctttt cgtgggcagt
    20641 tttcggaaag agttgcctct ctgaccagtt ttcttcttca gctaagaata atgagtctgc
    20701 acaatgagcg cattgcagat attgcattac atgaaaagga ggaaaagaaa cctgaaattg
    20761 aaatcgttgc tgatatgggg ccaatatccc tggaaaccaa tggtttaagc tatcgttatg
    20821 acagtcagtc agcaccgata ttcagtgctc tgagtttatc tgtagctccg ggggaaagtg
    20881 tggctataac tggtgcttcc ggtgcgggaa aaaccacatt aatgaaagta ctatgtggac
    20941 tatttgaacc tgatagcggg agggtactga taaatggtat agatatacgc caaattggaa
    21001 taaataatta tcaccggatg atagcctgtg ttatgcagga tgaccggcta ttttcaggct
    21061 caattcgtga aaatatctgt ggttttgcag aggaaatgga tgaagagtgg atggtagaat
    21121 gtgccagagc aagtcatatt catgatgtta taatgaatat gccaatggga tatgaaacat
    21181 taataggtga acttggggaa ggtctttctg gcggtcaaaa acagcgtata tttattgcac
    21241 gagccttata ccggaaacca ggaatattat ttatggatga ggcaaccagt gctcttgatt
    21301 cagagagtga acatttcgtg aatgttgcca taaaaaacat gaatatcacc agggtaatta
    21361 ttgcacacag agaaacaacg ttgagaactg ttgatagagt tatttctatt taaaccatag
    21421 aggaattaca agcgtatgag gaatatttct tcctgttata attcctcgtt atgctcagat
    21481 atctgttgga ggtggaatgg aagatagaca atccaccaag aagaaatatc attctgtgtg
    21541 gattgtccaa taactgttct ttcttatatt aaataatact atttataaac aaacatcact
    21601 aagattattt ggactccaat tacacaatct tcccgcagca tagttccatg cttctgaagg
    21661 tatcccttcg ggtttttgct taattgttcc ccctaaaccg gatggagaca ttgcaggatt
    21721 aggtttgtga gtggatgcat agtcatatat tgcacctcca gccacacccc cagcagctgc
    21781 tccaattcct cctgcaacaa attgcccgga tagtgttcct atagccatcg caatatcacg
    21841 ccctgaagca ccaccagaaa cagaatctaa ttcatttaga gtcagagttc tcatatgatc
    21901 tcctttttat cttatcggat attgaataat aattatcacc aacaaagtaa catattgcag
    21961 acattaatgc agagaagcaa aatgtatgca tggataaaaa gtcctttcct ctaaaaacac
    22021 aatcatatat agctaatgca atatatattg cggtggcatt tattataaat gcaaataaca
    22081 actctaattt tgttcttttt ctatccatta ctttttatcc cattaccaca caaacactaa
    22141 cgataatgat tatcgttaac atagtcaaga gtgaagggta ggaggccctc aaccccctat
    22201 aaggggtccg cttggaaaac ggatttcccc acgtcaagag aattgatttg aacgagtggc
    22261 ttcgctacaa acagctcccg tttgtgtgtg aaaaacccct ctcaacagat ctcaactctg
    22321 cacgatatct acaggagatt gctccaaaag gcagtatcgt gtcttttaac taccgatata
    22381 accccgtcat cgacatgata atgcgtctga gaaatgagaa gaaattaggt gaactgcatt
    22441 tcttctcagc agaatttaat aaaaattctg cactgacccg ccatcacctg acctggcgag
    22501 actccgcaca acagagcaaa agcagcggtg ctctgggcga tctatcctgt catctgcttg
    22561 atttattttg ttttataggg gaaagcccgg tagtggttca tgggatcaaa acggtaaagg
    22621 ggacacgtgg gtacaaaatc agatggtcag gttgaagtgg atgataacgg ctatgtaatg
    22681 ggcagttcag aaaagggagc ttatttcaga gttcatgcca gtaaatcgga aacagatcat
    22741 aatttgggac tgcatataca gcttgtattt gaaaatggtg aaatcagata ttcaacccac
    22801 catgaaaatc gcctgctcct gattctattt aatgatacga atacggaaac aattggcttc
    22861 gacgctctga aacgcttgcc tgatccacca cgggagcttc cattctggtc tgattcgttt
    22921 attcatcttc atgacgactg gtgtgctctt attaaatatg gtcactcgtc tcctaagcta
    22981 gcggacctgt catccggcct tcatatacag gaaataattg aggcattctg atatggatac
    23041 cattctttta accgggctgt ttgctgcatt tttcacaacg tttgcgttcg ctccccagag
    23101 cataaaaact atcaggacac gcaataccga aggtatctcc gttgttatgt atatcatgtt
    23161 cctgacaggt gtaatctcat ggatagcgta tggcattatg cgatctgatt ttgccgtact
    23221 gatcgccaat attgtcacac tgtttctggc tgcaccagtg cttgtaataa cacttatcaa
    23281 ccgcaggaaa aaacacgtat gaatattgca atcatcggcg cgggtcctgc cggcatcatt
    23341 tctgcccgta atgcgattaa agcaggtcac tccgtggttc tgtttgagaa gaatacccga
    23401 attggggaga tctggaaccc ctggagtggt ggcgcttacc gtaatgcctg tatgcagaac
    23461 tctcgttata catttcatta tactggcttt ccccctggcg atatcgatga atttctagga
    23521 gtggaacagg tattcagata tctttcggcc gtggccggag aggatgccct ccgtgagtcg
    23581 atccgactga atactgaagt tgtttcactc agaaaagacg ccggacaccg ggtgatccgc
    23641 tgcgcttctg aaggaaaaga cacggaagac atttttgacc gggtcatcat tgcaacaggt
    23701 gaactctggc aaccccgccg gccccccctg ccaggtgagg aaaacttctc cggaacgttg
    23761 atcacgtcga gagattatca ggagccagag gcatttaaag gaaaaaatat cctcatcatt
    23821 ggcggcggtg tcagcggtgc ggatattgcc tcagaccttg ttccctttgc cagaagcgta
    23881 agtctgtccg tcaaaaagat gggactttat ctgccgagac aattcccgac tggcccgaat
    23941 gacatgatgc actcctatct gggcaggtgt ctgctgagcc aaatgaatta tgaagatttt
    24001 atcggttatc tcgacaccat gatgcctgac tacatgcagg cctaccgagc atccagtctg
    24061 ttgcccgaca tggcgaacaa taacgcggtg catgttaacg aaaaaatcat accgaatgtg
    24121 gccgccggtc tgataaaagt taaaccccag gcagagcgtt tcaccggaga aggcgctatt
    24181 aagttcgtcg acgcatctca ggaaaaatat gatgtaataa taacctgtac aggctatgaa
    24241 atgcctgatt actcgttcat tcgtaagcgt acagcctgaa ccgtctggtc agaatctgac
    24301 gaattagaca aagtggtgtc caccaaataa gtagtgggaa ccaaagtgtc agatatgcag
    24361 aaaaatgtga ctcccggcag gcgaaaaggc tgccctaatt atcctcccga atttaaacag
    24421 ctgctcgttg ctgcctcctg tgaacccggg atatccatct caaaacttgc tcttgaaaat
    24481 ggcattaacg ccaatctgtt gttcaaatgg cgacaacaat ggcgcgaggg aaagctgcta
    24541 ttaccttctt cagagagccc ccagctactt cctgtgactc tcgatgcagc tgccgaacag
    24601 ccagaatcgc ttgcagagga cccggaaacc ctcagtatca gctgtgaggt aacgttccgg
    24661 cacgggacgc tccgcttcaa tggcaatgtc agcgaaaagc tcctgactct gctgatacag
    24721 gaactgaagc gatgatcccg ttaccttccg ggaccaaaat ttggctggtt gccggtatca
    24781 ccgatatgag aaatggcttc aacggcctgg ctgcgaaagt acagacggcg ctgaaagacg
    24841 atcccatgtc cggccatgtt ttcattttcc ggggccgcag cggcagtcag gttaaactgc
    24901 tgtggtccac cggtgacggg ctgtgcctcc tgaccaaacg gctggagcgt gggcgcttcg
    24961 cctggccgtc agcccgtgat ggcaaagtgt tccttacgca ggcgcagctg gcgatgctgc
    25021 tggaaggtat cgactggcga cagcccaagc ggctgctgac ctccctgacc atgctgtaaa
    25081 tctctttatc ctggttgtca cagaataagc ccggtaaaat acgggcttat gaacgacatc
    25141 tcttctgacg acatcttcct gctgaaacag cgcctggccg aacaggaagc gctgatccac
    25201 gccctgcagg aaaagctgag caaccgggag cgcgaaatag accatctgca ggcgcagctg
    25261 gataaactcc gccggatgaa cttcggcagt cgttccgaaa aagtctcccg ccgtatcgca
    25321 caaatggaag ccgatctgaa ccggcttcag aaagagagcg atacgctgac tggtagggtg
    25381 tatgacccgg cagtacagcg tccgttgcgt cagacccgca cccgtaagcc gttccctgaa
    25441 tcactacccc gtgacgaaaa gcgactgttg cctgcggcgc cgtgctgccc gaactgcggc
    25501 ggttcactga gctatctggg cgaggatacc gccgaacagc tggagttgat gcgtagcgcc
    25561 ttccgggtta tccggacggt acgggaaaaa catgcctgta ctcagtgcga tgccatcgtg
    25621 caggcacctg caccttcgcg gcccatcgag cggggtatcg ccggaccggg gctgctggcc
    25681 cgcgtgctga cctcgaagta tgcagagcac accccgctgt atcgccagtc agaaatatac
    25741 ggccggcaag gtgtggagct gagccgttca ctgctgtcgg gctgggtgga tgcatgctgc
    25801 tggctgctgt ctccgctgga agaggcgctt catggctatg tcatgactga cggcaaactc
    25861 catgccgatg ataccccggt ccaggtactg ctgccgggta ataagaagac gaagaccggg
    25921 cggttgtggg cgtatgttcg tgatgaccgc aatgccgggt cagcgttggc acctgcagtg
    25981 tggttcgctt acagcccgga caggaaaggc atccatccgc agactcatct tgcttgcttc
    26041 agcggtgtgc tgcaagcgga tgcgtacgcc gggttcaacg agctgtatcg caatggtcgg
    26101 ataacggaag ctgcctgctg ggctcatgcc cgccgaaaga tccacgatgt gcacgtccgc
    26161 atcccgtcag cactgacgga agaagccctg gagcagatcg gtcagttgta cgccatagag
    26221 gcggatataa ggggaatgcc ggcagagcag cggcttgctg aacgtcagcg aaaaacgaaa
    26281 ccgctgttga aatccctgga aagctggttg cgtgaaaaga tgaagaccct gtcgcgacac
    26341 tcagagttgg cgaaggcgtt cgcgtacgca cttaaccagt ggccggcact gacgtactat
    26401 gcgaacgatg gctgggtgga aatcgataac aacatcgctg aaaatgccct gcgggcggtc
    26461 agtctgggtc gtaaaaactt cctgttcttc ggctctgacc atggtggtgg cggggagcgc
    26521 tactgtacag cctgatcggg acgtgcaaac tgaatgacgt ggatccagaa agctaccttc
    26581 gccatgtgct tggcgtcata gcagactggc cggtcaaccg ggtcagcgaa ctgcttccgt
    26641 ggcgcatagc actgccagct gaataacaca tccccgtcaa tacggccctc gctgtacgct
    26701 taccaataaa taatatgctt atcgacctga tggctgcaat gtcgcataaa gactggctga
    26761 gtcgtcgtca acgacaaaaa cagggaattg aacgtgccca tactttaggt aagtatcggg
    26821 gcaaacaggc agatcgggaa cgacatcaga aagtaatgta ctaccggcag atcaagaaac
    26881 tgagcatccg tgaaacggca gaggtgacag gctatagtac ttcccaagtt tgtcgcattc
    26941 aggcgctcta taaagaggtt aaacctgatt aaaaaatagc cttaacgtac aatattttcc
    27001 gtttcccaaa cggagcccaa tacagatggc taacagaccg tctgcttcca gttcaaatgg
    27061 tttaccgtcg cggtccaggg cgcgaatttt aactttctct gcgcgtggca ctaaagctcg
    27121 ttgttcaggg attgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag
    27181 ctccggattg attaacacca gccgttcgtc acggttttcc gaaacatcaa ttacaatgat
    27241 acgttgatgg atatcaacct gggttgccgc caggccaata cctttttctg cgtacatcgt
    27301 ctcgaacata tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc
    27361 aactttgcga agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca
    27421 gagatgtgtt caggagttag aaagattatt tcttctactg taccgtaatc agataatcgc
    27481 agcaaaggga caaagcaata gtccctttgc tgcacagcat cagaatgaat aacgaatatt
    27541 agcgtttatc gcatcatctg tgttgtattt accaaatgca gagcgttcaa cttccagccc
    27601 caaacgcgta ttgtcgccaa accgggcatt taaccccaca ccgtaaagca tacgactgtc
    27661 ttttctgcca ttaatctgat gttctcccgc tgcatccttc agatgaacgt cagcactgtc
    27721 cgtcagatcg aactcataat gcaggccggc acgggctgtc agactccagt ccttaccact
    27781 gaaggtttta ccggaaacaa cgccggttct gcctaccaga ggattaacgc tgttacgacg
    27841 cattgagaca tccattccac tgtcgttcca gttaaatgtt tggccctgca gtcttcccca
    27901 gaccagttcc gcctgaggtt caacaaacgt cgtatctgtc agatgataac ggtatccgac
    27961 ttctgcacct gcatacagtg aatggctgcg gaagttctgt ttaccagctc cggcaaagtt
    28021 caggtcatat ttgttttcat tgtgaatata tttggcaatc acatcaaagt aagcgccgga
    28081 ccggaacaga ccactggcat agaaaccacc accccatgat tttgttttac cgctgtacag
    28141 gtctgctgac gcatctgtgt cagtgtaggt ggccatcacg ccggtaaaca ggtccatact
    28201 tcccagttcg tgcttacggt cagcccccat ctgcagcagg gtatagtggt cagtgaaacc
    28261 gccatcagca gagccggaac cgttcagcag acgcacccac gtaccggctt cgccattaat
    28321 atccctcaaa tcgcccatgc gtttgttcag gttgttaact tcagtgatga agttgttata
    28381 gctgatgtgc atgaatgtgg cggcagcctt accctggccg tcgttacgtg caacctggta
    28441 accatcgagg acccactctt ttttcccgtc ctcttttctg acactaagga tgggggtgac
    28501 atcactgaat cccacaaccc gtgttgatgc cctgaacaga ttatcagctg tcgcttcagg
    28561 tgcgctgacc agtggaatat caagcgtgtc cttgttagaa ggttttttca ggaagttaac
    28621 ccagatgctg ttgtcatgac ctgttgccga cttgtttatc accagtttgt ctgccttgtt
    28681 aaggtctgta cgcatgacaa atgctgactg aaccgcgtcc agattatctg ttgtcagtgt
    28741 cgtgaacggt gatgttcccc cgttaaaacc gactattgtc cggttaagtt tcatatttcc
    28801 tgccgtggag tttccgttca tcgaccactg ggtgtctgtc atgctgacgg tggcatccgg
    28861 tgcattcagg ctcccgctcc agatattgcg gtacccgtta aacaggctgt acaacatctg
    28921 attctgaaga gtcaggtcag gactcagttc cccttcccct ccgagggtga cagtcccttt
    28981 atcctgaaca ttgatattac ctgacaacat actgtacggc cccacgttca ggcgggcatc
    29041 gtctcccttc aggttccatg aaccggcata atcgtataca ggtaaaagtg tgtgagatac
    29101 ctcatcagga cggctgttca gactcagggc ggcatcagaa atattcactg gaccgtcaga
    29161 aacaaaactc tgactggcca gagcatttgc tcccttgttc agattcaggg cggtactggt
    29221 cagtgttgag ttccccagaa cggcactgtc tgaggagata ttcacggtac tgttgttcgc
    29281 ctgtattccg ccattgaata tatcattgat attcagcact gactgattat ccaggttgac
    29341 ggtgccgttg aagacacttt tatctgcatc tttagttgca acagatgtgc cttcttcaag
    29401 ggtaaaggct gttccctggc catcgttttt gtcgataaat acccggctgt cgcccagcgt
    29461 gacgctggag ttatctgcct ggatggttgt gttcagtgtg gcattgcgac caagaccaaa
    29521 atcagtgttc ttcagtgaca gcctgtcaaa ggtaaaactg cggttctccc agtcctcctg
    29581 actgaatgac gtaggctgag tcagaaccga atggtctcca ctggcagcca gtttgtcagc
    29641 cacagactga gtattgtatg catggataac cggatgcccc tgcagcgtca gacgcccgtt
    29701 ttcctgggtg aatgtaccgg agatatccgc agctccgtcc atcaccagag caccggtaac
    29761 gccttcaggc aggcgattgt ccacattcag attgcctttc agttgtccgt gaaacagata
    29821 ccctgcagta ttgaaacggt cagctaccag tttctgtgca tccccctgac tgtgccccac
    29881 aaactcccag gtggcgttgc tgctctggtc cgtggggaaa taaccatagg tgctctgctt
    29941 caggatgaag taatccgtcg tatttgtgta cgggttattg tatttatata aatttccggc
    30001 agttccttta cctgattccg accagccatt cagtgctact ttgtcagccc gcagggcata
    30061 gtccagcgtg atagtggccc gtttatcaac gttattcgcc agcacggcac cataatctgc
    30121 cgccttcaac tgatgaaacg tcagactgtt accattaaca tccagtgtgc ccccacgata
    30181 tccccatgag acggtatccg gatttacctg ccgctcatca gtcagtacca cggtcggccg
    30241 gccactggca atattaacac tgctgaacgc ctgcacctgt cctttattgt ccgtctgctg
    30301 gttcagtaca acctttccgt ccccgacctt caggccacct tcattaatac ctgtaccctg
    30361 taccgtcagc gtaccttcac caattttatg caggttatcg cccttaacac cattaacctg
    30421 ccagtttacg gacaccccgt tgtccacaac aataccggca ccggtccagg tacttccgtt
    30481 agaggttgtt actgtgtaat tatcacggaa cgtcagggaa cccgccccct gagaaaccga
    30541 atccttaagg ttaatctgac cattctgccc ctgaaatatc aggttcttac cagcattcag
    30601 gtcatttccc tgctgcccgt gcatggcata tgtggtggtt ccctgtgtca gcgcaccagc
    30661 tccggtactg ctgttaaagc tccactccag tgcaccacct tccgatgttc tgaacgtgac
    30721 cggggcatca ttgtcttcat taaatttctg cccgataaaa tccagtggaa taacagccca
    30781 gttatttccc ctgccccccg cgccattccc cgcagtaaga acaccgacca gcacccattt
    30841 attctgaaca gtatcaaaag caaataaagg cgaaccgctg tcaccggcct cgccataaat
    30901 gggcattgca ccattaagtt tgtaatcaaa aaccagacct gaactggtgc taatcatttc
    30961 tccattctga taggatgaca ggctaccgac agtcccgccg gtcagccagg aatatgcacc
    31021 tcccatttgt gtcagctgtc cgttactgtc cttaatatac tgagtaccag accccagacg
    31081 ataaaaaaca ggatagcgct ccttatccag atatgcgcca gccactgccc cctgcgccgt
    31141 caccgcagta ggggcaacct ctgtcaccag tttatccagc cggggggcat gaaaatccag
    31201 tgacggcgca ttattccggt ccacgatatt gtaacggttt tcaccatcac caaagctaac
    31261 gtttgtatat cccccgttat gtttcacgct ggcgatatac tgcgggttta tcagtgtcgc
    31321 cacaccgatt tccgaatcca cagcactgaa atcaggcata gctgccttat ccagcgtacc
    31381 gacaaattct ccctgcttat tataaatagc gatattcgtt gcccccgggc ggaacatccc
    31441 cttattttca gcaaaatcac gaaataactg atacccgagt tcattattga ccgttcccgc
    31501 aagacttcct gcagagaata gtaccgggat cagtaataaa accgggaaac acagacgtct
    31561 gacagactta tgaacacatt tcctggcaaa ctcagatacg gcaataaagc cccgggccac
    31621 agcgctgtag cgaagagaat aaattctgtt catttttaat tactcctgag aaaaataaaa
    31681 tcagtactac tcaggcataa cggataatca ccatcatcct gaggaatccc cagaaaaagg
    31741 agacaggcat agcgtgatgt gaactactga ttctttcaca aattcaggga gagcaccgtt
    31801 atgcctgcac ataaagtatg ccagaaccgg tggcgaatgt gtgctatgca caaatggtga
    31861 aacagttcct gagtcgtgac ccgctcgaat gcgtgctgtg tggcggccga atggtatacc
    31921 ggcgtgccat agcggggctg aatgtggacg ggctgaagaa aaacgcgcgg gatatcagtc
    31981 tgataaggat tactttccag ccggctgatg gctgacgggc gtaatcccat tcgttcggcc
    32041 agcgcagact ttgttaaccc cgctttttct ctcatttcgt ataacatttc taccatctcc
    32101 agttctttgt cagcatcttc atacccttta acagcttctg gagtattaag cagtttttgc
    32161 tttacctggc tgaacggaac actttttact ttcgtcattt catctccttc aggcgcatcc
    32221 ttgaagtggt ttactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
    32281 cacaggtgct ccaatgaaaa aaagaaattt cagcgcagag tttaaacgcg aatccgctca
    32341 actggttgtt gaccagaact acacggtggc aga
//
LOCUS       DAEVZA010000042        31652 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEVZA010000042 DAEVZA010000000
VERSION     DAEVZA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.042"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1200)
                     /gene="yiaN"
                     /locus_tag="LAF92_002047"
     CDS             complement(<1..1200)
                     /gene="yiaN"
                     /locus_tag="LAF92_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HBI9791055.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYV"
     gene            complement(1203..1676)
                     /gene="yiaM"
                     /locus_tag="LAF92_002048"
     CDS             complement(1203..1676)
                     /gene="yiaM"
                     /locus_tag="LAF92_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HBI9791056.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            complement(1794..2261)
                     /locus_tag="LAF92_002049"
     CDS             complement(1794..2261)
                     /locus_tag="LAF92_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBI9791057.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKGLDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            complement(2273..3271)
                     /gene="yiaK"
                     /locus_tag="LAF92_002050"
     CDS             complement(2273..3271)
                     /gene="yiaK"
                     /locus_tag="LAF92_002050"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBI9791058.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            3472..4320
                     /gene="yiaJ"
                     /locus_tag="LAF92_002051"
     CDS             3472..4320
                     /gene="yiaJ"
                     /locus_tag="LAF92_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBI9791059.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            4422..4895
                     /locus_tag="LAF92_002052"
     CDS             4422..4895
                     /locus_tag="LAF92_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBI9791060.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(5047..6300)
                     /gene="avtA"
                     /locus_tag="LAF92_002053"
     CDS             complement(5047..6300)
                     /gene="avtA"
                     /locus_tag="LAF92_002053"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBI9791061.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEEGVKILAEEIERAWAESH"
     gene            complement(6478..8508)
                     /gene="malS"
                     /locus_tag="LAF92_002054"
     CDS             complement(6478..8508)
                     /gene="malS"
                     /locus_tag="LAF92_002054"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBI9791062.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            8828..9652
                     /locus_tag="LAF92_002055"
     CDS             8828..9652
                     /locus_tag="LAF92_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBI9791063.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(9760..10938)
                     /gene="xylR"
                     /locus_tag="LAF92_002056"
     CDS             complement(9760..10938)
                     /gene="xylR"
                     /locus_tag="LAF92_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HBI9791064.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(11016..12197)
                     /gene="xylH"
                     /locus_tag="LAF92_002057"
     CDS             complement(11016..12197)
                     /gene="xylH"
                     /locus_tag="LAF92_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBI9791065.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNFQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIILTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(12175..13716)
                     /gene="xylG"
                     /locus_tag="LAF92_002058"
     CDS             complement(12175..13716)
                     /gene="xylG"
                     /locus_tag="LAF92_002058"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBI9791066.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(13794..14786)
                     /gene="xylF"
                     /locus_tag="LAF92_002059"
     CDS             complement(13794..14786)
                     /gene="xylF"
                     /locus_tag="LAF92_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI9791067.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            15152..16474
                     /gene="xylA"
                     /locus_tag="LAF92_002060"
     CDS             15152..16474
                     /gene="xylA"
                     /locus_tag="LAF92_002060"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBI9791068.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            16546..18000
                     /gene="xylB"
                     /locus_tag="LAF92_002061"
     CDS             16546..18000
                     /gene="xylB"
                     /locus_tag="LAF92_002061"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBI9791069.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWATKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            18169..18510
                     /locus_tag="LAF92_002062"
     CDS             18169..18510
                     /locus_tag="LAF92_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791070.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            18556..18993
                     /locus_tag="LAF92_002063"
     CDS             18556..18993
                     /locus_tag="LAF92_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBI9791071.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKEYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(19035..20030)
                     /gene="wecH"
                     /locus_tag="LAF92_002064"
     CDS             complement(19035..20030)
                     /gene="wecH"
                     /locus_tag="LAF92_002064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBI9791072.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            20205..20504
                     /locus_tag="LAF92_002065"
     CDS             20205..20504
                     /locus_tag="LAF92_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBI9791073.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            20599..21510
                     /gene="glyQ"
                     /locus_tag="LAF92_002066"
     CDS             20599..21510
                     /gene="glyQ"
                     /locus_tag="LAF92_002066"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBI9791074.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            21520..23589
                     /gene="glyS"
                     /locus_tag="LAF92_002067"
     CDS             21520..23589
                     /gene="glyS"
                     /locus_tag="LAF92_002067"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBI9791075.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            23915..24067
                     /gene="hokA"
                     /locus_tag="LAF92_002068"
     CDS             23915..24067
                     /gene="hokA"
                     /locus_tag="LAF92_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBI9791076.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(24255..24467)
                     /gene="cspA"
                     /locus_tag="LAF92_002069"
     CDS             complement(24255..24467)
                     /gene="cspA"
                     /locus_tag="LAF92_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBI9791077.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(24748..25038)
                     /locus_tag="LAF92_002070"
     CDS             complement(24748..25038)
                     /locus_tag="LAF92_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBI9791078.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            25472..26182
                     /locus_tag="LAF92_002071"
     CDS             25472..26182
                     /locus_tag="LAF92_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBI9791079.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(26232..27206)
                     /gene="ghrB"
                     /locus_tag="LAF92_002072"
     CDS             complement(26232..27206)
                     /gene="ghrB"
                     /locus_tag="LAF92_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBI9791080.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(27310..27969)
                     /locus_tag="LAF92_002073"
     CDS             complement(27310..27969)
                     /locus_tag="LAF92_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBI9791081.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            28122..30455
                     /gene="bisC"
                     /locus_tag="LAF92_002074"
     CDS             28122..30455
                     /gene="bisC"
                     /locus_tag="LAF92_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBI9791082.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(30424..30864)
                     /locus_tag="LAF92_002075"
     CDS             complement(30424..30864)
                     /locus_tag="LAF92_002075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI9791083.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPRAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(30861..31424)
                     /gene="tag"
                     /locus_tag="LAF92_002076"
     CDS             complement(30861..31424)
                     /gene="tag"
                     /locus_tag="LAF92_002076"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBI9791084.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPTSDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            31582..>31652
                     /locus_tag="LAF92_002077"
     CDS             31582..>31652
                     /locus_tag="LAF92_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBI9791085.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAF"
BASE COUNT         7899 a         7771 c         8045 g         7937 t
ORIGIN      
        1 gacgtaaggg aaaacgcctc tgactgcatc atcgaatttg agttttgcca cccctgaaat
       61 aacgttaagt acattaccga taggcggtgt aattaagccg attgagcagt taatgataaa
      121 catgacaccg aaataaatcg gatcgattcc tgcctcttta actaaaggca ttaataccgg
      181 ggtaagaatt aataccgtcg gcgttaaatc catgaccatg ccgacaatca ggatagccac
      241 cataatgacg ataaacagca gacgcggtga atcgaccaac ggttgcagta aatcagaaac
      301 catcatcggc agttcagcaa tggtaatcag ccaggcggaa acttgtgccg aggccaccag
      361 aaacataacc actgaggtgg ttttcgccgc gccaatgagt acatgccaaa gtgtggcgaa
      421 ggtcatttca cggtaaataa ctgtggcgac aaacagcgca tagaaagcag caacggcccc
      481 ggcttcggta ggggtaaaca gaccggagcg aaacccgcca ataatgatga ctggaagaaa
      541 cagcgcccag ataccggaga caaaagagtg ccagatttcc tgcatcgttg ctttttgctg
      601 gcggggtaaa ttaaggcgac tggcctgcca ccaccaggta agcatcagcg ttgcgcccat
      661 catcatgcca ggggcaatgc ccgccataaa cagcttactg atggataatc cgctggaaac
      721 gccgaagata ataaacggaa tggaaggggg aataattggc gcgataatgc cgccagaggc
      781 aatcagcccc gccgcccggt tgaccgggta gttggcgctg cgcatcatcg gcaccagtag
      841 agcggcaacg gcggcggtat ccgcaacggc agaaccagaa aggctcgcca taatcatagc
      901 tgccagcacg ccgacgtagc ccagcccgcc tggtttatgc cccaccagtt tcatcggcag
      961 gtcaacaatg cgttttgaca agccgcccgc attcatgatt tcacccgcca gcacaaagaa
     1021 cggaatcgcc agcagggaga agctatcggc tccgttcacc agcgtttgtg ccatgatctg
     1081 gacatcaaac atgtccagcc agaacattaa cgccgccccg cacaacaaca gtgcccaggc
     1141 aataggcaaa ccgatagcaa taccacccaa cagacagccc agaaaaatca gcacagccat
     1201 gattaagctc cttgcggtgg cgacgttaga gaattgctac gcgtgatgag ttgatataaa
     1261 tgacgcagtt caaagaatgc gataacgagg ctggtgggca gacaagcggc atacatcagg
     1321 ccgatgggta aaccgaggat cggtgaataa tcgctccagt cctgaattgt ttttagcgtt
     1381 gcgccccagg ccagtgcgcc acaaataaat aagattaagg aatgggtaac cagagcgact
     1441 cgtcgttgcc atgcggggga gagtttctcc accagaaagg tgacctgaac gtgggcgtta
     1501 tccataaaag ccacaatcgc gccaataaat gttaaccaga caaataaata acgtgacaat
     1561 tcatctacag ataaaatgct tgtctgaaaa ccatatctta aaataatgtt tataaacaca
     1621 atacaggaaa gtacggcgag attaatcgcc agtattgctt caagtatttt tttcatagct
     1681 attccttgag gctatgtctg tcataattca agttgcatgt gcagcaacct aaattattaa
     1741 tatagttaga gcaataacat tacccagtcg ttctggcgta gacgattatt agattaattc
     1801 agcgccgtta atgcgacttt aaccacaatt tttcgtatct cagaggctgt ttgcaaaata
     1861 caacctggtc ggtgaacatc ctgcggaaag aaaattgcgt agctgcccgg tatcatttct
     1921 ataaatgatt catgttcact gtcatgataa aaaataatat cgcgctgctc taatagtgat
     1981 tcgctgactt tattatttcc cgtatcaata gcaatgccga ttttctcttc accccacgcc
     2041 agaaactgaa tatcgatata ccgacgatgc acttccggac ggttttccac cgcttcgcgt
     2101 gtggttaaat cgataatttg cgcataaata tttttgccgt cgatttcgac aacgcccggc
     2161 tccagggcgt tgaaatcggt agcgcgcaga aaatcgagcc ccttttcaat ggcggcgggc
     2221 aaacggcacg gattgggctg cgcgatatgt ccaaaaatca tgacttatct ccttataacg
     2281 cctggatttt ggcccacacg ctgtcatcaa cggtaatacc gttacggcgg ttttcggcca
     2341 gcagggtagt aaattcgtgg ccgggtaagc ggatggcctg gttttcgtca gcgcgttcag
     2401 cggtagtaac gtaatccatg atacgttgca gcttggcatc gcgggtggga ccgtcgataa
     2461 gcttgtccac ttcaatggca ataaaaattt gtgaaatgcc gtattcgtcg ctgttgtcct
     2521 gggtgacttc ggcaaccgat gcgccgtcgg aaaggagagt agcgatcata tccagcacaa
     2581 tcgacatgcc agaacctttc cagtagccca tcggcaaaat gcggcgattc ttctcgataa
     2641 cgccaggttc tttggtcaaa ttgccctcat catcaaagcc accatcgacc gggagctgac
     2701 ggcccgccag gcggttaact tctaacatgc cgtaagagaa catcgacatc gacatatcga
     2761 ccatggtgat cggcgtggaa ggaatggcga cgatcagcgg gttagtgcca atgcgacact
     2821 cttttgcgcc ccacggcggc attacggcga tggagttggt ccagcaaatg ccaatatagc
     2881 ctttttccgc cgcctgccag ccgtagctgc cgccgcgcat ccagtggttg gcattacgaa
     2941 gtgccaccag accaataccg tgatcggcag ccagttcaat ggcgcgatcc atcatctttt
     3001 tcgccgtcag gttgccgatc gaacgctggg catcccactg ttcaattgcg ccgaggctgg
     3061 ttatacgttt gggttgggca tcaggaatga tatcgccgtt ttccagttgt tgaatgaaac
     3121 gagggaaacg attaacgccg tgagaataaa cgccggattc ggtggtgcgg gcgaacatct
     3181 ctgcacaggc gtcagccgtt tcgctgtcaa cgccgcgtga aattaagacc cgattaaagg
     3241 ctgcttttaa ctgctcaaat gtcactttca tcccaggctt ccttgttttt ttgatcgctt
     3301 ttttggctta aaatttcaat atgcgaaact tgatttcaaa tatatcgatc actttttaac
     3361 aaagcaatct gattgatgaa tttcaaaaga cataaaaatc aagtggttat aaattatctg
     3421 tcgagatcgt gaactacggc acactttgcg ctaccatcag gacgcgacaa aatggggaaa
     3481 gaagtgatgg gaaaaaaaga gaacgagatg gcgcaggaaa aagagcgtcc agccggaagc
     3541 cagagtctgt ttcgcgggtt gatgctgatt gagattttga gcaactatcc aaacggttgt
     3601 ccgttggcgc atctttcgga gctggctggt ttaaataaga gtaccgtcca tcgcttattg
     3661 cagggattac agtcctgcgg ctatgtgacc accgcgcccg ccgcagggag ttaccgcctg
     3721 actaccaaat ttattgccgt cgggcagaag gcgctgtctt cgctgaatat cattcatatc
     3781 gccgctccgc atcttgaggc actgaacatc gccactggtg aaaccattaa cttctccagc
     3841 cgcgaagacg atcacgctat tttgatttat aagctggaac ccacaaccgg gatgctgcga
     3901 acccgtgcct atattggcca gcatatgccg ctctactgtt ccgcaatggg caagatctac
     3961 atggcgtttg gtcacccgga ctacgtgaag tcatactggg aaaaccatca gcatgagatc
     4021 cagccgttaa cccgcaatac cattaccgag ctgcccgcga tgttcgacga actggcgcac
     4081 attcgtgaaa gcggagcggc gatggacaga gaagaaaacg aactcggcgt ctcctgtatt
     4141 gctgttccgg tgtttgatat tcatgggcgg gtgccgtacg ccgtgtcgat ttcgctttcg
     4201 acatcacgtc tgaaacaggt gggagagaaa aatctcctga aaccactgcg tgaaaccgcg
     4261 caggctattt ctaatgaact gggatttact gtcagggatg acctgggcgc gatcacataa
     4321 cgctttggac aagtgccaaa actttaacat ttccttcgtt ggatcaaagc agtagggacg
     4381 cgctctctgg cactctgctg ttttagtgca aaggagtgat catgaaccgg tttattattg
     4441 cggatgcgac gaaatgtatc ggttgccgta cctgtgaagt ggcttgcgca gtgtcgcatc
     4501 aggagaatca ggattgcgct gcgttgtcac cagacgagtt tatttcccgt attcgtgtca
     4561 ttaaagacca cagctggacc acggcagtag cctgtcatca gtgtgaagat gcaccgtgcg
     4621 cgaatgtctg ccctgttgac gcgataagcc gcgaacatgg gcatattttc gttgaacaaa
     4681 cacgttgcat tggctgtaaa agctgtatgc tggcttgccc gtttggtgcg atggaggtcg
     4741 tttcttcgcg caaaaaggcg agggcgatta agtgcgatct gtgctggcat cgggagacgg
     4801 gaccggcctg tgttgaagcc tgcccgacaa aggcgttgca gtgcatggat gtcgagaaag
     4861 tgcagcggca tcggctacgg cagcagcctg tttgaaacgt ttttccggcg tttctcaacg
     4921 ccggaatagt gcgctttgtt tatgccagat gcggcgtaaa cgccttatcc ggcccacaaa
     4981 ttcttgcaaa ttcaatatat tgcagaaatc atgtaggcct aataagcgta gcgcatcagg
     5041 caatttttag tgactttcag cccaggctct ttctatctct tccgccagaa tcttcacccc
     5101 ctcctcaatt ttctccggct ctggtacgta gttcatgcgc atacattgat gcgtatgcgg
     5161 ccacggtttg tccagccctg ggaagaagtt gtgccccggc accatcagca cgccgcgtgc
     5221 tttcaggcgc tgatagagct gctcggtcgt aatgggcaaa tccttaaacc aaagccagag
     5281 gaaaatggct ccttctggtt tatgaattag gcagcgatct tccggtaaat agcggcgaat
     5341 gatggcgata gtttcctgaa cacgctggta gtaaaacggt ttgatgactg tttcagacag
     5401 gcgcagcaga tcgttacgct taatcatttc acacatcatc gccggaccaa taccgccagg
     5461 tgccaggctg ataatgccgt tcatattggt gatggcggtg atgatttttt cattggcgat
     5521 gataatgccg cagcgggagc caggcagccc cagcttggaa aggctcatgc acagtacgat
     5581 attcggattc cacagcgggc gcgcttcgct gaagatgata cccgggaacg ggacgccata
     5641 agcgttatca atcaccagcg gaatgccgtg ttgattcgcc agcgcgtcaa gcttcagcaa
     5701 ctcttcgtca gtaatcacat tgcctgttgg attcgtcggc cgggagacgc aaatcatccc
     5761 ggtttcttcg ccaatatgca gatgctcaaa atcgacgtgg tatttaaact ggccttccgg
     5821 cagcagttca atattcggac gcgcagagac aaacagatct tcttccagtc cggcgtcagc
     5881 gtagccaatg tattccggtg ccagcgggaa cagcactttt ttgacccgac catcggcacg
     5941 gcgtccggca aacagattaa ataagtagaa aaacgcgctc tggctgccgt ttgttagtgc
     6001 aatattctgt gcttcgatat cccaacccaa cttctcgcgc agcattccgg caagcagtgt
     6061 gagtagctcc gttttcccct gtggaccgtc gtaattgcac agtgcatcag tcgctttgcc
     6121 actttccagc atgtcggtca gtagcgtctg gaagtagtcc tgcatttccg ggatctgcgc
     6181 cggattaccg ccgccgagca taatcgcgcc aggcgtgcgt aaaccgtcgt tcagatcttc
     6241 catcagcagc gtaatgccgg agtggcgggt aaatttgtca ccaaaaaggg agaatgtcat
     6301 agcgggatat ctgtcgaact ctgtagcaag gaaggtaaca ataacgctac atttgctggg
     6361 gtgcaaatcg gtctgttgaa gagtgagcgg tgctttatcc tgcaacgctg attagggctg
     6421 acattttatc ctggttggcg gcttcccgag gtactgacga acgggaagcc ggaaaagtca
     6481 ctgttgccca gcccagatga ccagcacttt atcgtcgcca tgctcacgaa caaagccgta
     6541 gccctgcttc agcgaaagtg tcgtttgttt gcccgcgcca attgcgggat ggcgggcgcg
     6601 gaactggctg attttctgcc agtgcgcgac gttggcggca gatttaccgc taacatcctg
     6661 ccagttcata tccgaacgtg taccttgcag cggatcagaa cctgtaggac cgaacggacg
     6721 cgaggattca tcgccataaa agatttgtac cgcgcctggc gctaatagta ataactctgc
     6781 tgctttgtcg cccccttcac ggaacagacg ggtatcatgc gacgagaggt agctcaacac
     6841 gttgaaaccc tgcaatttct ccgccatttg ctgccaggtc gtatccatct gcgccagaca
     6901 atcgactgct ttcgccgcct gctcctgata atcgaaattg atcatcgcat cgaagccgtg
     6961 gcgatagtag tcactttgca tcacgccgtg gccccaggct tcaccggtca tccagaaagg
     7021 tttgtcatct aatgctttgt cggggttagc ttttttccat tcgcgaagcg cggcgctggc
     7081 ttcggttttc agttgctgcc aggcgggcaa ctcaacatgt ttggcggtat cgacccgaaa
     7141 accatcaatc ccatagtcgc ggacccactg acttaaccag tgggttaagt aatcgcgagg
     7201 tgtaaagcct tcgatgactt tagcgtgagt atccgttttg tttttataga acaccggcag
     7261 accagaagcg gtagttgatt cggttttgat atccggcaaa aaggccagcg acatagtgag
     7321 atcgtcgaat ccaggattgt cgtaatcgcc gatatccgtt ctgatccagt tttttcccca
     7381 ccatttatcc cagcctgttt tgtcgctgaa attaatgtaa tcgttaaagc tatgccaggt
     7441 ttgcccggcg gcaggtttcc agtcgctcca gcgttcaccc agcgtttttt tcacctcgtc
     7501 accagaaaga tataacgcgc caaactgata ctcctgcata tccgccagcg tggcatagcc
     7561 ggtgtggttc atcacgacat caaagagaat acgaataccg cgctgatgtg cgctatcaac
     7621 cagcgtccgt agatcggctt cgttgcccat attggcatca agattcgtcc agtcctgtgt
     7681 gtaataaccg tggtaggcat aatgcgggaa atcgcctttt gtaccgccgc cgacccagcc
     7741 gtgaatttgc tcaaatgggg cgcttatcca taaagcatta acgcccaact gctggaggta
     7801 atccagtttg ttggtcaggc cgcgtaaatc gccgccgtga aaagtgccaa tttccgccat
     7861 accgtcttta tgacgtccgt aactctggtc attactggga tcgccgtttt cgaaacgatc
     7921 tgtcagcaca aagtaaaccg tggcgttatg ccagtcgaaa ggggcagggg cgtcagtttc
     7981 tgcccgttcc agcaggagta aaccgttgct ggtagcagcg ggttgtaacg ttatttgacc
     8041 gttcttcact atcgcaattt gctggctgta ataatcccgt acggcggctc cttccgggaa
     8101 agtggcgctg acatccagcg tgagcggtaa tccatcccat ttcgggcatt cacggaccag
     8161 gtttgctacc ggttcggcgg cgttctggat ggaaatcatc aatgttggcg taccggagcg
     8221 ggtgtctatt tgcagcgtat atttgccgtc tctgaacaat cgccattgag gcggcgtgtt
     8281 gctacaaggt tgcagggaaa gcatctgatt gagttttatc gcatctgcag gctgccagca
     8341 ctgttggtca aaatttagcg tgagtggacg cgtacctttg ggcaactgcg cgtggctgac
     8401 aaatgttccc gttccctgtt cgctaaaggc agggaacccc ggagaagtcc agctggcggc
     8461 aacggcgaag ccaggaagga gtgtcagaaa acaggcggcg agtttcatgg tggatgagtc
     8521 cttattgctg cgatttcaga cagtttgcca gcgataagcc agacaaaact catccctgag
     8581 cggtaagtcc acaggatgag aagcaagggt gagcgatctc gcgcaaaaaa cggctgaatt
     8641 ttgcgataaa ccccacattt tctgcgattt agcgccaatc tgaatcgtta acacgtgaca
     8701 tagtttcaga tttggactat tccttggggt gctcttgaca gctatttttg atatgatttg
     8761 agattcaact ctcaaatttg tgaaaaatat aaggtgttgg aatgattaaa tccgaccagg
     8821 agacctgatg attttgactc ccatacgacg atatggggcg atgattctta tgttactcac
     8881 tctggtgttt tcgagtgagg tgttagcgaa gacgcacaca acaacagcga gtcaaaagtc
     8941 ccacttaact aaagctagta ataaacaggt aagcagtaaa caagagtatt ctcgcaatag
     9001 tgcaaagagt agttcacttc ctgatttgcg aaaataccct tccggaacac caaggaaaaa
     9061 ggcgtttctc cggaccgtaa tgccttacat taccagccaa aatgcagcca ttactgcaga
     9121 acgtaactgg ctgatttcaa aacagtatca gggccaatgg tcacctgctg agcgtgcgcg
     9181 tctgaaagac atcgccaaac gctacaaggt gaaatggtct ggtaatacgc gaaaaatccc
     9241 gtggaatacc ttgcttgaac gcgtagacat tatccctacc agtatggtgg cgacgatggc
     9301 tgcagcagaa agcggttggg gaacgtcgaa gctggcgcgc aacaataaca atctgttcgg
     9361 catgaaatgc atgaaaggac gttgtaccaa tgcgccgggt aaagtgaaag ggtactcaca
     9421 gtttagttct gtcaaagaat cggtgagcgc ctatgtcact aacctgaata cgcacccggc
     9481 ttactcttcg ttccgtaaat cgcgtgcgca gctgcgtaaa gcggatcagg aagtgactgc
     9541 cacagcgatg attcacaagc tgaagggcta ctcgaccaaa gggaagagtt ataataacta
     9601 cctgttcgca atgtaccagg ataaccaacg gttaatcgcg gcgcatatgt aattgcattt
     9661 ccttgagcct tatctgattt gtcggtcgga taaggctttt gctcgcacca ggtggctgtg
     9721 ctgagttccc tgatgtgacg ccgacaattc tcatcatcgc tacaacatga cctcgctatt
     9781 tacatcgcga tactcttttg gcgttgtgtc atatgctttt ttaaaaacag agtagaaata
     9841 ttgcagcgat ggataaccgc acatttgcga tatctcattg atcgacaagg tggttgaaat
     9901 cagcagactg cgcgctttct ccagcttctc ggcatgaatc atggcatgga tggtttcacc
     9961 cacctcttct ttaaaacgct tctcaagatt ggagcgcgag atcccgaccg catccagtac
    10021 ctgatccact ttaatccctt tacaggcgtg attacgaatg taatgcatgg cctgaataac
    10081 ggcgggatcg gtcagcgagc gataatctgt tgagcgccgt tcaatgacgc gaactggtgg
    10141 gaccaaaatt cgctgtagcg gcatttcttc tttatctaat aatcgatgca acagttttgc
    10201 cgcctgatag cccatttgcc gcgcgccctg agcgaccgaa gaaagggcga cacgcgacag
    10261 atagcgggtc agttcttcgt tatcgatgcc aatcacgcat aatttttccg gtacgggaat
    10321 atgtagatgt tcacatactt gcagaatatg ccgcgctcgg gcgtcagtaa cggcaataat
    10381 cccggtttgc ggtggcagcg tttgtagcca gtctgccagc cgattttgcg cgtgttgcca
    10441 gttctctggc gcggtttcta acccctgata aaccactccg cgatactttt cttcagcgac
    10501 aagctgacga aatgcatatt cgcgctcagt ggcccaacgt ttgccgcttg attccggaag
    10561 accataaaaa gcaaagcggt taacgccttt ctcttttaaa tgcaaaaatg cgctttcaac
    10621 cagcgcatag ttatcggtgg caatgtaatg aacgggtggg taactttctg caaggtgata
    10681 cgagccgcca accccaacaa tggggacgtc gacatcagcc agcgcttgct cgatctgttt
    10741 gtcgtcgaag tcggcaatga cgccatctcc taaccagtcc ttgattttat caatgcgggc
    10801 gcggaaatct tcttcaatga aaatatccca ttccgattgt gacgcctgta aatattcccc
    10861 tacgccttct actacctgcc ggtcataggc tttattggca ttgaacagta atgtgatgcg
    10921 gtgacgttta gtaaacatgg ttcttttcct gctgaatcat gcaaaaactc aaaaccggta
    10981 atacgtaacc ggctttgaga aaatttttat caaaatcaag aacggcgttt ggttgcggag
    11041 tccatccata ctgccagcaa cagaatcgca cctttaacga tatactgcca gaaggtcggt
    11101 acatccatca tactcatgcc gttatccagt gaagccatga taaatgcccc cattactgct
    11161 ccggcaacgc ttcccacacc gccagccagg ctggtgccgc caatcacgca tgctgcaatt
    11221 gcgtccagtt cggcgatatt tcccgcagaa ggtgaaccag cgccaagtcg agaactaagg
    11281 attaatccgg cgatggctac cattaatccg ttaatcgcga acacggcaag tttggtgcgt
    11341 tcaacgttaa tcccggagag acgtgccgct tcaagattgc cgccgatggc ataaatgcgt
    11401 cgtccaaatg ccgtccgcgt tgccataaac attccgccga gtaacagcaa cgtcagcagc
    11461 agaacaggag tgggaacgcc acggtaatca ttcaacagcc agattgcgcc taatacgatg
    11521 atagcggtta aagcctggcg accgactact gcggtagagg ccggagactg taaacccaaa
    11581 gcctgacggc gcattcttcc gcgccattgc caaccaacaa aagccattaa gccaagcgcg
    11641 ccaatgatga agccggtact ggcggggaga tagctttgtc caatttgtga catcgcggcg
    11701 ctggtggggg atacagtcgt gccgttggtg atgccaatga gtatgccgcg aaatgccaac
    11761 atgcccgcga gggtgacaat aaatgaaggg actttacggt acgcgaccca ccatccgttc
    11821 cacgcaccga gaagcagtcc cagaaccaac gtcagaatga tggtaagtgg caaaggccag
    11881 cctaaccaga cgtcacaaat cgccgcgacg ccacctaata gccccatcat tgagccgacg
    11941 gaaaggtcga tttcagcaga aattatgacg aacaccattc ctaccgcgag gatgccggta
    12001 atcgcggtct ggcgtaacag gttggagacg ttacgggcgc ttaagtaggc accatcggtg
    12061 gtccaggtaa agaacagcat gattgcgatg atagctgcaa tcatcacgaa gacctgaaaa
    12121 ttcagtgact tcagcccaga aaaaccaccg gatgtcggta cggccaattt cacttcagac
    12181 ggattgcttt tcgacatgat gttcgctcct caatgcggct tccatcacct gctcctgagt
    12241 caggttatga tttatcaggt tggcttttag tttcccttca tgcatcacca gtacccgatc
    12301 gctaaggccg agcacttcag gtaattcgga agagatgaca ataacggcaa taccctgctg
    12361 gacgagttgg ttaattaatt tgtagatctc gtatttcgcg ccaatatcga tacccctggt
    12421 aggttcatca agaatgagga tgcgcgggtt aagtaacaga cagcgagcga ggatcgcttt
    12481 ttgctgattg ccgccgctca aacgtccaat agcaaggtcg ggggacgacg ttttaacttt
    12541 gagttgctga attgattcca gaatacattt ttgctctgcc gcgtcatcaa gctggctaat
    12601 gccaccggta aatttattga gtgcggcgag ggtaatattt ttaccaaccg ccattaccgg
    12661 aacgatgccg tcgcgctttc tgtcttcggg taccatcgca atcccctggg cgatggcttg
    12721 ctgacagtta cgaatatcta cctgtttgcc atcaatataa atttttcctt cccattgtcc
    12781 gggccacacg ccaaacaggc actgaatggt ctcggtacgt ccggcaccaa cgagtccggc
    12841 aatacccagt atttcgccac gtttcaggga aaacgagaca tcattaactc ttttaatatg
    12901 acgattaacc ggatgccatg ccgtcagatg ttcaatacgt aatatttcat ctccggtggt
    12961 atgtggttca ttagggtaaa gcgcggttaa ctctcgcccg accatcatgg tgataatatc
    13021 gtcttcactc attccggcag catcacgcgt accaatgtgc tgaccgtcgc gaataacgca
    13081 aatcgtatcg gaaatcgctt tgacttcgtt gagtttgtgc gaaatataaa tacaggcgat
    13141 accgtgctgt tgtagatcgc gaataatatc cagtaaaacc gacgtttcct gctcagttaa
    13201 tgaggctgtc ggttcatcga gaattaacaa gcgcacctgt ttattaagtg ccttggctat
    13261 ttcaaccagt tgttgttgcc caagccctaa atctcccacg cgggtatctg gtgaaatgga
    13321 taaactgacc tgtgcgagca gcttctgaca gcgtagcgtc atcaggtcat aatccataat
    13381 accattgtgg gttatttcgt tacccaggaa gatattttcc agtacggtca attctttcac
    13441 cagggccaat tcctggtgaa tgatggcgat acctttgcgt tcggtatcgc ggatgtgact
    13501 cgcctgaatc tcttctcccg caaaaataat ttcgccttcg taggagccat ggggataaat
    13561 accacacagc actttcatca gcgttgattt accagaccca ttttccccac agagtgagac
    13621 gatttcgcca gcattcaacc gcaagctgac gttatcaatc gccttcacac tgccgaaggt
    13681 tttggtaatg ttcttcattt caagtagata aggcataacg actccaccta agccaattaa
    13741 ttcacgcggc atggagagaa atcacgcccc cgctccgcgc cggggcgtaa cgcttacagc
    13801 tcgctctctt tgtggaatcc gtctttaact accgtgtctt tgatgttgtt tttattcaca
    13861 tcgatcggtg tcaggaggcg ggagggcaca tctttcaggc cattattcag tgtggtatct
    13921 gcttttggtt cctgaccatt gcccaactca acggcaattt ctgcggcagt atttgccagc
    13981 aatgtaatag gtttatacac cgtcatagtt tgcgtaccgg cagcaatacg tttaatacct
    14041 gcgagatccg catcctggcc ggaaattgct actttccctg ataaaccttg cgcgcttaat
    14101 gcctgaattg ccccacctgc ggtggcatcg tttgaggcaa ctacagcatc aattttgtta
    14161 ttattggcgg ttagcgcgtt ttccataatt ttcaatgcgt tttccggtaa ccagccatcg
    14221 acccattggt caccaacgac tttaattttt ccggaatcaa cgtaaggttt taacactttc
    14281 atttgtccgg cgcggaacag cttggcgttg ttatctaccg gcgagccgcc catcaggaag
    14341 taattacctt gcggaacaat atcgaccagg gcttttgcct gcagttcacc gactttttcg
    14401 ttatcgaaag aaatataaaa atcgatatcc gcatcgttaa tcatacggtc gtaagctaat
    14461 actttaatac cttcttgttt ggcttctttt acaacgttac ttaatacctg accgttatac
    14521 ggaataatga caagaacatc gacaccccgg ttaatcatgt tttcaatctg cgacatttgt
    14581 gtttcttcat tgccatttgc agactgtaca aatactttcg cgccgagaga ttctgccttt
    14641 ttcacaaaga tatctcgatc tttttgccag cgttcaagac ggagatcatc aatcgccata
    14701 cctattttga cttctttggc gtgtgccgca acgttggtaa gcagaagtga ggtgcaaagg
    14761 gtgagtagaa tgttctttat tttcatggtg tagggccttc tgtagttaga ggacagcttt
    14821 aataagtaac aatcaccgcg ataaacgtaa ccaattttta gcaactaaac aggggaaaac
    14881 aattacagat ttttatcttt cgattacgat ttttggttta tttcttgatt tatgaccgag
    14941 atcttacttt tgttgcgcaa ttgtacttat tgcatttttc tcttcgagga attacccagt
    15001 ttcatcatcc cattttattt tgcgagcgag cgcacacttg tgaattatct caatagcagt
    15061 gtgaaataac ataattgagc aactgaaagg gagtgcccaa tattacgaca tcatccatca
    15121 cccgcggcat tacctgatta tggagttcaa tatgcaagcc tattttgacc agctcgatcg
    15181 cgttcgttat gaaggctcaa aatcctcaaa cccgttagca ttccgtcact acaatcccga
    15241 cgaactggtg ttgggtaagc gtatggaaga gcacttgcgt tttgccgcct gctactggca
    15301 caccttctgc tggaacgggg cggatatgtt tggtgtgggg gcgtttaatc gtccgtggca
    15361 gcagcctggt gaggcactgg cgttggcgaa gcgtaaagca gatgtcgcat ttgagttttt
    15421 ccacaagtta catgtgccat tttattgctt ccacgatgtg gatgtttccc ctgagggcgc
    15481 gtcgttaaaa gagtacatca ataattttgc gcaaatggtt gatgtcctgg caggcaagca
    15541 agaagagagc ggcgtgaagc tgctgtgggg aaccgctaac tgctttacaa accctcgcta
    15601 tggcgcgggt gcggcgacga acccagatcc tgaagtcttc agttgggcgg caacgcaagt
    15661 tgttacagcg atggaagcaa cccataaatt gggcggtgaa aactatgtcc tgtggggcgg
    15721 tcgtgaaggt tacgaaacgc tgttaaatac cgacttgcgt caggagcgtg aacaactggg
    15781 ccgctttatg cagatggtgg ttgagcataa acataaaatc ggtttccagg gcacgttgct
    15841 tatcgaaccg aaaccgcaag aaccgaccaa acatcaatat gattacgatg ccgcgacggt
    15901 ctatggcttc ctgaaacagt ttggtctgga aaaagagatt aaactgaaca ttgaagctaa
    15961 ccacgcgacg ctggcaggtc actctttcca tcatgaaatt gccaccgcca ttgcgcttgg
    16021 cctgttcggt tctgtcgacg ccaaccgtgg cgatgcacaa ctgggctggg acaccgacca
    16081 gttcccgaac agtgtggaag agaatgcgct ggtgatgtat gaaattctca aagcaggcgg
    16141 tttcaccacc ggtggtctga acttcgatgc caaagtacgt cgtcaaagta ctgataaata
    16201 tgatctgttt tacggtcata tcggcgcgat ggatacgatg gcgttggcgc tgaaaattgc
    16261 agcgcgcatg attgaagatg gcgagctgga taaacgcatc gcgcagcgtt attccggctg
    16321 gaatagcgaa ttgggccagc aaatcctgaa aggccaaatg tcactggcag atttagccaa
    16381 atatgctcag gaacataatt tgtctccggt gcatcagagt ggtcgccagg agcaactgga
    16441 aaatctggta aatcattatc tgttcgacaa ataacggcta actgtgcagt ccgttggccc
    16501 ggttatcggt agcgataccg ggcatttttt taaggaacaa tcgatatgta tatcgggata
    16561 gatcttggca cctcgggcgt aaaagttatt ttgctcaacg agcagggtga ggtggttgct
    16621 tcgcaaacgg aaaagctgac cgtttcgcgc ccgcatccac tctggtcgga acaagacccg
    16681 gaacagtggt ggcaggcaac tgatcgcgca atgaaagctc tgggcgttca gcattctctg
    16741 caggacgtta aagcattggg tattgccgga cagatgcacg gtgcaacctt actggatgct
    16801 cagcaacggg tgttacgccc tgccattttg tggaacgacg ggcgctgtgc gcaagagtgc
    16861 actttgctgg aagcgcgagt tccgcaatcg cgggtgatta ccggcaacct gatgatgccc
    16921 ggatttactg cgcctaaatt gctatgggtt cagcggcatg agccggagat attccgtcaa
    16981 atcgacaaag tattattacc gaaagattac ttgcgtctgc gtatgacggg ggagtttgcc
    17041 agcgatatgt ctgacgcagc tggcaccatg tggctggatg ttgcaaagcg tgactggagt
    17101 gacgtcatgc tgcaggcttg ccacttatct cgtgaccaga tgccagcatt atacgaaggc
    17161 agcgaaatta ctggtgcttt gttacctgaa gttgcgaaag cgtggggtat ggcgacggtg
    17221 ccagttgtcg caggcggtgg cgacaatgca gctggtgcgg ttggtgtggg aatggttgat
    17281 gctaatcagg caatgttatc gctggggacg tcgggggtct attttgctgt cagcgaaggg
    17341 tttttaagca agccagaaag cgccgtacac agcttttgcc atgcgctacc gcagcgttgg
    17401 catttaatgt ctgtaatgct gagtgcagcg tcgtgtctgg attgggccac gaaattaacc
    17461 ggcctgagca atgttccagc tttaatcgct gcagctcaac aggctgatga aagtgccgag
    17521 ccagtttggt ttctgcctta tctttccggc gagcgtacgc cacacaataa tccccaggcg
    17581 aagggggttt tctttggttt gactcatcaa catggcccca atgaactggc gcgagcagtg
    17641 ctggaaggcg tgggttatgc gctggcagat ggcatggatg tcgtgcatgc ctgcggcatt
    17701 aaaccgcaaa gtgttacgtt gattggtggc ggggcgcgta gtgagtactg gcgtcagatg
    17761 ctggcggata tcagcggtca gcagctcgat taccgtacgg ggggggatgt ggggccagca
    17821 ctgggcgcag caaggctggc gcagattgcg gcgaatccag agaaatcgct cattgagttg
    17881 ttgccgcagc taccgttaga acagtcgcat ctaccagatg cgcagcgtta tgccgcttat
    17941 cagccacgac gtgaaacgtt ccgtcgcctc tatcagcaac ttctgccatt aatggcgtaa
    18001 acgttatccc ctgcctgacc gggtggggga taattcacat ctatatatct cactaattaa
    18061 ttaatattta gtatgaattt attctgaaaa tcatttgtta atggcatttt tcagttttgt
    18121 ctttcgttgg ttactcgtaa tgtatcgctg gtagatatgg agatcgttat gaaaacctca
    18181 aaaactgtgg caaaactatt atttgttgtc ggggcgctgg tttatctggt tgggctatgg
    18241 atctcatgcc cattgctaag tggaaaaggc tattttcttg gcgtgttaat gacagcaact
    18301 tttggtaact atgcatatct tcgcgcagaa aaactcgggc aactggataa tttttttacc
    18361 catatctgcc agttagttgc gttaatcact atcggtctct tgtttatcgg tgttttaaat
    18421 gcacctatca atgcttatga aatggtgatc taccccatcg ccttttttgt ctgcttgttt
    18481 ggtcaaatgc gtttgtttcg ctcggtatga gcaacataaa gctcttacac attcaggaat
    18541 gaaaggaata ctgtgatgga caacaaaata tcaacctatt caccggcctt tagtattgtg
    18601 tcatggatag ctctcgttgg tggtatcgtt acctatctgt tagggctatg gaatgcagag
    18661 atgcagttaa atgaaaaaga gtattatttt gccgtactgg tgttaggact gttttctgct
    18721 gcgtcttatc aaaagaccgt tcgggacaag tatgaaggca taccgaccac ttccatttat
    18781 tatatgacct gcctgactgt ctttattatc tctgttgcat tactgatggt aggtctgtgg
    18841 aatgcgacat tattactcag tgaaaaaggt ttttatggac tggctttctt cttaagcttg
    18901 tttggtgcag tagcggtgca gaagaatatt cgtgataccg gaataaaccc gccaaaagaa
    18961 acacaggtta cccaggaaga atacagcgaa taattcacgt aagcccggtc agtccaatgt
    19021 gaacgggctt ttacttaact cactaatctg tttctgtcga ttcgttgtac cagcatagaa
    19081 agtaacaaac tcgctgccaa cgtcgcgcaa aagatccaaa taatatccag tattggccaa
    19141 tttttaagct caattccccg ggtgcgcagc gcatggataa tcaaggcgtg gaatccgtat
    19201 atacccaatg aatggcggga gattaagcca agtccgcgaa tggtacgcgt atccagcgtg
    19261 tttttaacca gagtcaatag cgcgattgcg cagataaaaa ccatcggccc acagtaaaga
    19321 taccaggtat cggcaaaatt tccgcgccac tgcaattcat ataatgtccc gcgagagata
    19381 ataaaaaccc ccgtcgcaaa cagcgcggcg ctcacccacg acagtgcttt atgctgtgtg
    19441 tccatcatcc ctatagcgcg gcccaacatg ccatacagaa tgtagtaaaa agtatcgcca
    19501 ttgatatata agttaattgg cagccattca aaaccgtcaa ttttctgcgg cactgtgttt
    19561 gggttagcga taatgccaat caccaccatt agcaccagca acatttttcc gccgacgttc
    19621 ttcacctgaa tcagcggtga aaccagataa atcaccgcaa tcgcgaagaa aaaccacaag
    19681 tggtaaaaca ctggcttttg cagcaggttt ttcagcgcta actccatatt gatggaggta
    19741 aacagcgcaa tgtagagcag tgcgattgcg ctataaaaaa tcagacataa gccgatacgc
    19801 aagaaatggc gcggctgggc gctgcgttcg ccaaaaaaga gatagccgga aatcatgaaa
    19861 aatagcggca cgctgacacg agaggcagaa ttcagaacat tggcgatatc ccaggtgacg
    19921 gggctaacac tatgagcatt ggtcacatac caggtagtgg tgtgaatcat caccaccatt
    19981 aaacacgcta tccctcgcag gttatcaatc cagtaaattt tgggctgcat ctgtgtctct
    20041 gtatctggtt aaaaaaagtc tgaccgataa atcattcagt tggcgcactg gaataatctg
    20101 agttttatca ctacagctta tagaggctta aggaaattcg taagatatca gccactatac
    20161 cgatataaat aataagactc acctgcaaac cagacggtaa tttaatgatg atgaacgctt
    20221 tctttccggc aatggcgctt atggtgctag tgggttgttc tacaccgtca cccgtgcaga
    20281 aagcacaacg ggtaaaggtt gatcctctgc gttcgttgaa tatggaagcg ttatgcaagg
    20341 atcaggcggc aaaacgttat aacaccggcg agcaaaaaat cgacgtcacc gccttcgaac
    20401 agttccaggg aagctatgaa atgcgcggtt ataccttccg taaagagcag tttgtctgtt
    20461 cttttgacgc agatggccat tttttgcatc tttccatgcg ttaagccctg ctttttcccg
    20521 tttcgtactg tatatcttcc atccagcggg tatactgatc ccttccttta aatccacacg
    20581 tatccagcac gaaataatat gcaaaagttt gataccagga ccttccaggg cttgatcctg
    20641 accttacagg attactgggc tcgccagggc tgcaccattg ttcaaccatt ggacatggaa
    20701 gtcggcgcgg gaacctctca cccaatgacc tgtctgcgcg cgctggggcc agaaccgatg
    20761 gcggctgctt atgttcagcc ttctcgtcgc ccgaccgatg gtcgctacgg cgaaaacccc
    20821 aaccgtttac agcactacta tcagttccag gtggtcatta agccatcgcc ggacaatatt
    20881 caggagctgt acctcggttc tctgaaagag ctgggcatgg acccgactat tcacgacatc
    20941 cgtttcgtgg aagataactg ggaaaacccg actctgggtg cctggggact gggctgggaa
    21001 gtgtggctaa acggcatgga agtgacgcag ttcacttact tccagcaggt tggtggtctg
    21061 gagtgtaaac cggttaccgg cgagatcacc tacggtctgg aacgtctggc catgtacatt
    21121 cagggcgtag acagcgttta cgacctggtc tggagcgacg gcccgctggg taaaaccacc
    21181 tacggcgacg tgttccatca gaacgaagtg gagcagtcca cttacaactt cgaatacgcg
    21241 gatgtggact tcctgttcac ctgctttgag cagtacgaga aagaagcgca gcagctgctg
    21301 gcgctggaaa atccgctgcc gctgccagcc tacgagcgta ttctgaaagc cgcccacagc
    21361 ttcaacctgc tggatgcgcg taaagccatc tccgtcaccg agcgtcagcg ctacattctg
    21421 cgcattcgca ccctgaccaa agcagtggca gaagcatact acgcttcccg tgaagccctc
    21481 ggcttcccga tgtgcaacaa agataagtaa gaggcggcta tgtctgagaa aacttttctg
    21541 gtggaaatcg gcactgaaga gctgccacca aaagcactgc gcagcctggc tgagtccttt
    21601 gctgcgaatt ttactgcgga gctggataac gctggcctcg cacacggcac cgttcaatgg
    21661 tttgctgctc cgcgtcgtct ggcgctgaaa gtagctaacc tggcggaagc gcaaccggat
    21721 cgtgaaatcg aaaaacgcgg cccggcgatt gcccaggcgt tcgacgctga aggcaaaccg
    21781 agcaaagcgg cagaaggttg ggcgcgtggt tgcggtatta ccgttgacca ggctgagcgt
    21841 ctgactaccg ataaaggcga atggctgctg tatcgcgccc atgtgaaggg cgaaagcacc
    21901 gaagcactgc tgccgaatat ggttgcgact tctctggcga aactgccgat cccgaaactg
    21961 atgcgttggg gcgcaagcga cgtgcacttc gtgcgtccgg tgcacaccgt gaccctgctg
    22021 ctgggcgaca aagtcattcc ggcaaccatt ctgggcattc agtccgatcg cgtgattcgc
    22081 ggccaccgct ttatgggcga accggaattc accatcgaca acgccgatca gtatccggaa
    22141 attctgcgtg agcgcgggaa agtcatcgcc gattacgaag aacgtaaagc gaagattaaa
    22201 gccgatgccg aagaagcagc gcgtaagatt ggcggtaacg ctgacttaag cgaaagcctg
    22261 ctggaagaag tggcttcgct ggtggagtgg ccggtcgttc tgaccgcaaa attcgaagag
    22321 aaattcctcg cggtgccggc tgaagcgctg gtttacacca tgaaaggtga ccagaaatac
    22381 ttcccggtgt atgcgaacga cggcaaactg ctgccgaact ttatcttcgt tgccaacatc
    22441 gaatcgaaag atccgcagca gattatctcc ggtaacgaga aagtcgttcg tccgcgtctg
    22501 gcggatgccg agttcttctt caacaccgac cgtaaaaaac gtctggaaga taacctgccg
    22561 cgcctgcaaa ccgtgctgtt ccagcaacag ctgggtacgc tgcgtgacaa aactgaccgc
    22621 atccaggcgc tggctggctg gattgctgaa cagattggcg ctgacgttaa ccacgcaacc
    22681 cgtgcgggcc tgctgtctaa gtgtgacctg atgaccaaca tggtcttcga gttcaccgac
    22741 acccagggcg ttatggggat gcactatgcg cgtcacgatg gcgaagcgga agatgtcgcg
    22801 gtggcgctga atgagcagta tcagccgcgc tttgccggtg atgacctgcc gtctaacccg
    22861 gtagcctgtg cgctggcgat tgctgacaag atggatactc tggcgggtat cttcggtatc
    22921 ggccagcatc cgaaaggcga caaagacccg tttgcgctgc gtcgtgccgc acttggcgtg
    22981 ctgcgaatta tcgttgagaa gaacctcaac cttgatctgc aaacgctgac cgaagaagcg
    23041 gtgcgtctgt atggcgataa gctgactaat gccaacgtag ttgatgatgt tatcgacttt
    23101 atgctcggtc gcttccgcgc ctggtatcag gacgaaggtt ataccgttga caccatccag
    23161 gcggtactgg cgcgtcgtcc gactcgtccg gctgatttcg atgcccgtat gaaagcggta
    23221 tcgcatttcc gtaccctgga tgcagctgct gcactggcgg cggcgaacaa acgtgtatct
    23281 aacattctgg cgaaatctga cgaagtgctg agcgaccgcg tgaatgcctc taccctgaaa
    23341 gagccggaag aaattaaact ggcgatgcag gttgtggtgc tacgtgacaa gctggagccg
    23401 tactttgcgg aaggtcgtta ccaggatgcg ctggtcgaac tggcagagct gcgtgagccg
    23461 gttgatgctt tcttcgataa agtgatggtc atggttgatg acaaagaatt gcgtctcaac
    23521 cgtctgacca tgctggagaa actgcgcgaa ctgttcctgc gcgttgcgga tatttcgctg
    23581 ttgcagtaat aacgccgtta ttaaaaagcc tgccatctgg ccggcttttt ttattatcgc
    23641 taaataatac agcaaccttt aataatcttc tgctgaataa agattatctc atatattaat
    23701 tttatgaggt ttttttagga ttatatcaag gagaagaaac aaacttatta agctagaata
    23761 gccacgggtg cttgagactg tttgtctcag gtattcaccg aaaggcagac agagaaaagc
    23821 cccacctgac tataaatcaa agtgaggcta ccctatgcct gaacaccata aggttagcct
    23881 cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc
    23941 tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg
    24001 tgaattgcat attaaacagg ggagttatga gctggcggca tttttagcct gcaatttaaa
    24061 agagtaagag tcttcggcgg gaagttattc ccgcctttct tacggcgttg cgcattctca
    24121 ttgcacccaa atttattttt cacaaaaata atactagatt ttattacgcg atcgattatt
    24181 tatttcctga aaaacaaata aaaaaatccc cgccaaatgg cagggatctt agattctgtg
    24241 cttttaagca gagattacag gctggttacg ttaccagctg ccgggccttt agcgccgctt
    24301 tcgatggtga aggacacttt ctgaccttcg tccagagatt tgtaaccatc gttctggata
    24361 gcagagaagt gtacgaacac gtctttagag ccatcgtcag gagtgatgaa gccgaagcct
    24421 ttgtcagcgt tgaaccattt tacgatacca gtcattttac cggacatagt gtattacctt
    24481 taataattaa gtgtgccttt cggcgatatg gcgtgcttta cagattttga agcgttaaag
    24541 gaatgtgcac tacgaggggt atcaacgata actcttgaag ggacttgcct tactacactg
    24601 ctttaatggt ctgtacgtca aaccgttgat gtgcattaag ccacgcattg gcgggtgatg
    24661 caacaattat ttttcatatt tatgattaat cggttggaaa acggtcctgt catcaggacc
    24721 gtaaacagca ataaagtgga taaaagtcta ttccatcaac tgcttactta atgccgggtt
    24781 ggcttgaatc aaacgcatca attttagttc ggcacttgaa ggcttcacgc gtctggattc
    24841 ccattccttt accatggcga ctgatacgcc caaaacccgg gcgaaatcat cgatttttaa
    24901 ccctgtccct tttcgtaact gctcaatttc ggtacaggac gttgttctgt gcgtcagggt
    24961 aattttctgc gtttcatctt taaaaacaat ctgttccagg ctgctcaaca gctcatgcat
    25021 tggatcttta tattccattg aaaactcctc aaatcacact gcgggatcgt gaattgcatc
    25081 gaagctcatt aagaatagtc gggaaaagca gacacagcgg ggtgaagtca gggcatgctc
    25141 tggctatgat tcgattcagc ctgtcattta gcgtgactta tagccaccta acgatctgat
    25201 aaatgccgat atatctctta ccactcctgt tttgtaaaga tttcgaaaat ctaaccttct
    25261 gcgaaggcgt aaatctctgg ctacaaaaaa ggcaacggca ctatcatttt ttaacaatga
    25321 aacaacaaat taaccttgtt tcattttctc catgatttta ccaggaagat tacgtagaaa
    25381 gggtatcctg caagcctgtc ctggactatc cttgtcacgt cagacacgcg tgtgtcgttg
    25441 tgcgcttttt ttgggtgaaa ggagtaagaa aatggcgaca ggaaagtcct gctctcgctg
    25501 gtttgcgcct cttgcggcgt tattaatggt agttagcctg agtgggtgtt ttgataaaga
    25561 aggcgatcag cgtaaagcgt ttatcgactt cctgcagaat acagtgatgc gtagcggtga
    25621 acgtctacca accctgactg ccgatcagaa aaaacagttt ggtccttttg tctctgatta
    25681 cgcgattctg tatggttatt ctcagcaggt gaatcaggcg atggattccg gtctgcgccc
    25741 ggtcgtagac agcgttaacg caattcgcgt accgcaggat tacgttacgc aaagcggtcc
    25801 gctgcgtgaa atgaacggtt cgctgggtgt gctggcgcaa caactgcaaa atgcgaagtt
    25861 gcaggccgat gctgcgcact ctgcgctaaa acagaccgat gatttgaaac cggtctttga
    25921 tcaggcattc accaaagtgg taacgacgcc agctgatgca ttgcaaccgt tgattccggc
    25981 tgcacaaact ttcacgcaac aactggtgat ggttggagac tacattgctc agcaaggtac
    26041 tcaggtgagc tttgtcgcaa atggcatcca gttcccgact tcacagcagg caagtgaata
    26101 taacaaactg attgcgccat taccggcaca acatcaggcg tttaatcagg cctggactac
    26161 agcagttact gcaacccaat aaagagtaaa agcccgagcg attatcgggc tttacttcac
    26221 gcagtcgcgg cttagtccgc gacgtgcgga ttcacacagt tcttctcaac ctttccttgt
    26281 aacgcatcaa tcaaattatc cacggcacag gcggccatgc cataacgcgt ctcatgggtg
    26341 gcagatccaa tatgcggtac tgcgacgacg ttggccattg agagcaacgg cgaatctacg
    26401 gacagtggct cttgttcgaa gacatccagc ccggcggcgt gaatttcgcc tttctgcaat
    26461 gcagcaatca gtgcattttc atcaaccacc gggccacgtc cggcattaat gaaaatggcg
    26521 gaggatttca ttttggcgaa ttgttctgcg ccaaacagat gatgcgtctc atcagttaac
    26581 ggcaggatca ggcaaacgaa atctgactct tgtaacagag tatccaaatc gcagtagcgg
    26641 gcgttgaagc gttcttctgc ttctttatgg tggcggcgcg cgttatagag gatgggcatg
    26701 ttgaagccaa agtgcgcacg ttgtgccagc gccataccga tccgtcccat cccgacaatg
    26761 cccagtgttt tatggtgaac gtcagtgccg taccagtccg ggcctatgct cgcggtccat
    26821 tcgcctgctt ttacccgttc tgctacctcc acaacccgac gagcggtaga caacaccagc
    26881 gccatcagcg tatcggcgac ggtttctgtt aatacggttg gcgtgtgcat cagcagaatt
    26941 tttcgggcgg taagcgcatc gacatcaaaa ttgtcatagc cgacggagat cgttgatgtg
    27001 gcacgcagtt tcggcatttt ttccagcaat gcggcatcaa cattctcgtt tgaacccagt
    27061 aaaccttcag cttcggcaaa aattgctgca ttttgttcga cggtttgtgg gctgaggttt
    27121 gccacctggt gaacggtgaa atgctcttgc aggcgttgca gtaaatcatc aggtaaggct
    27181 ttgtagagga taacggacgg cttcatgctt ctctccattc actgataagg cgatcacctt
    27241 agcctgaaat atgatgaagg aaaactgaag aatgccaggt gatttctgca tcacctggca
    27301 tgaaagggat tacagcgggc ttaaggtaat ttctacacgg cggttttgcg ccttaccttc
    27361 tgcggtgctg ttgctggcga ttgggttagc cgggccaagg ccctgagtac ggatgcggct
    27421 ggcgtccacg ccctgggtga tcaacgcgct ggcaacggaa tccgcacgtt gctgggagag
    27481 acgcatgttc aggtcgtgac cacccgtgct gtcggtataa ccaatcacgt taaccgccgt
    27541 tttcggatac tctttcagca ccattgccac gccggtcagg gtgttagcgc ctgccggttt
    27601 cagggtcgcg ctgctgctgt cgaaggtcac attgttcggc atattgagga taatgttatc
    27661 cccgctgcgg gttacgctaa caccagtgcc gcgcattttg tcacgcagct tcgcttcctg
    27721 cacatccatg taataaccca cgccgccgcc cagagctgcg cctgctgctg cgccaatcag
    27781 cgcgccttta ccgcgatctt tcttcgaaga agagagcgca ccaatacccg cgcccacgag
    27841 agagcccaga cctgcgccga tagcagattt gcctgcttcg cgttcgccgg tgtaagggtt
    27901 agttgtgcag ccagataccg ccagagcgcc actcactacg gcggcaataa gataaacacg
    27961 tttcttcatt gttaatcctt aataaccttt ttattctttg ccacgggttc cgtggcggga
    28021 gattatgccg cgtgaacatg aagattattc ctgggaatac tcggaaattt gtaagtaata
    28081 tttaactgct caatacatct aacctttcag gagtcttcgg tttggccaac tcatcctcac
    28141 gatattccgt tctgactgcc gcccactggg ggcccatgct ggttgaaacc gacggcgaaa
    28201 ccgtgtttag ctcgcgtggc gcgttagcca caggaatgga aaactccttg cagagcgcgg
    28261 ttcgcgacca ggttcacagc aatacgcggg tacgatttcc aatggtgcgc aaaggctttc
    28321 tcgcgtcacc ggaaaacccg caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt
    28381 gggatgaggc gctggatctt attcaccaac aacataaacg cattcgtgag gcttatggtc
    28441 cggcatcgat ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg cataaggcct
    28501 cgacattatt acaacgctat atggcgctgg caggcggtta taccgggcat ctgggggatt
    28561 attcgaccgg cgcggcacag gcgatcatgc cgtatgtcgt gggtggtagc gaagtttatc
    28621 aacagcagac cagttggccg ctggtgctgg aacatagcga tgtcgtggtg ctgtggagtg
    28681 ctaatccact caatacgctg aaaattgcgt ggaatgcatc tgatgagcag gggctttctt
    28741 acttttctgc actgcgtgac agcgggaaaa agctgatctg cattgatccg atgcgatcgg
    28801 aaaccgtcga tttctttggc gataaaatgg agtgggtggc accgcacatg ggcaccgatg
    28861 ttgcgctgat gctggggatc gcccatacgc tggtggaaaa tggttggcac gacgaagcgt
    28921 ttctggcgcg ttgcaccaca ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg
    28981 acggaatagc gaaaaacgcc gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa
    29041 tccgcgagct ggcggctatt ttccaccaaa ataccaccat gctgatggca ggctggggaa
    29101 tgcagcgcca acagtttggt gagcaaaaac actggatgat cgtcacgctg gccgcaatgt
    29161 tggggcaaat cggcacaccc ggcggcggtt ttggtctttc ttaccatttt gccaatggtg
    29221 gtaaccccac gcggcgcgct gcggtactct cttccatgca gggcagcttg ccgggtggct
    29281 gcgatgcggt agataaaatc cctgtggccc gcattgttga agcactggaa aaccccggtg
    29341 gcgcatatca acacaacggt atggaccgac atttcccgga tattcgtttt atctggtggg
    29401 cgggcggtgc caactttact catcatcagg ataccaatcg cctgatccgt gcctggcaaa
    29461 aaccggagct ggtggtgatc tctgaatgct tctggacggc ggcggcgaaa cacgcggata
    29521 tcgttctgcc tgcgactacc tcttttgagc gtaatgatct caccatgacc ggtgattaca
    29581 gtaatcagca tctggtgccg atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg
    29641 attttgatgt ttttgccgag ttaagtgaac gctgggagaa gggcggttat gcacgtttta
    29701 cggaaggaaa aagtgagctg caatggctgg aaacgtttta taacgttgcc cgacagcgcg
    29761 gggcaagcca gcaggttgaa ttgccgccat ttgctgagtt ctggcaagcc aatcagttaa
    29821 ttgagatgcc ggaaaacccg gacagcgagc ggtttattcg cttcgccgat ttttgccgcg
    29881 atccgcaggc gcatccatta aaaaccgcca gcggtaagat tgaaatcttc tcacagcgta
    29941 ttgccgatta cgcttatccg gattgcccgg ggcatccaat gtggctggag ccggacgaat
    30001 ggcagggcaa tgccgaaccg gaacagttac aggtactttc tgctcatccg gcacatcgtc
    30061 tgcacagcca gctgaattac agttctctgc gcgaattgta cgcggtggca aatcgtgagc
    30121 ctgtcaccat tcatcctgac gatgctcagg cgcgcggcat acaagatggc gatactgttc
    30181 ggttgtggaa cgcacgcggg caaattcttg ccggagcggt cattagcgag ggaattaaac
    30241 ctggcgtgat ttgcattcac gaaggggcat ggccggatct ggatttaacc gctgacggta
    30301 tttgtaaaaa cggcgcagtg aacgtgctga ccaaagatct ccccagctcg cggctgggga
    30361 atggctgtgc gggtaatacg gcgctggcat ggctggaaaa atacaacggt ccggaactga
    30421 cacttacagc gtttgaacca ccggccagct cataatccag gtgggtagtt gggtttcatc
    30481 ctgccatgcg caatcgacaa tgtgaaaacc ctgcgcacgg taaaaatcga tcgccggttg
    30541 atttttttga taaacctcca gcatcagatg gggatggcgc tgctgcacat actgcatcag
    30601 cgccttacca ataccgcgcc tgacggccct cggtgcgaca aacatcgctg ccagaaatcg
    30661 gccttccata atgctgacaa aaccgagaag cttactgtct tcttcccaga cccagttttg
    30721 cgcattggca agatacgcat cccgcaccag cggaatgcaa tcacgccagt aattcgcttt
    30781 tataaaggga tgcccccagg ttgtactttc cagccacagt tcgaggatcg cggggagttc
    30841 tgaacgttgc gcttcccgaa tcatggttta tttcccggat agcaacagca gccaaccaca
    30901 tgatcattca ccagcccaca tgcctgcata aaggagtaac agattgtggt accgacaaac
    30961 ttaaaaccac gttttttcag tgctttagat agggcgtcgg aggtgggcgt agaggtgggg
    31021 atttcgctca acgttgtggc ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca
    31081 aagtcggcaa acggttcgcc gttctgttcc atttgcaggt acgcccgcgc attaccaata
    31141 attgcctgaa ttttcccgcg atggcggata atcccggcgt cctgtaccag tctttcgaca
    31201 tcctcttcct gcattgcggc aaccttcacc ggatcgaact gatgaaagca ggcgcgatag
    31261 ttttcgcgtt ttttgaggac ggtgatccac gataacccag cctgctgccc ttcaaggcag
    31321 atcatttcga acagtttttt actgtcagtt tcaggtacgc cccactcatt atcatggtag
    31381 gcaatataaa gcgggtcctg actcacccag ccgcaacgtt ccatactttc cctcgctatg
    31441 tacccgatcg taaaaatttc agccgctttt cttgacgcat cggtgaaaaa gtcaatattt
    31501 aatacagggc tactatgccc gatatccttc atacaatgac aataatattg ccgaattcgg
    31561 ctcttccctg gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc
    31621 ctgctggcga gcgtggtaat cagtgccttt tt
//
LOCUS       DAEVZA010000043        31566 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEVZA010000043 DAEVZA010000000
VERSION     DAEVZA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.043"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(57..133)
                     /locus_tag="LAF92_002078"
     tRNA            complement(57..133)
                     /locus_tag="LAF92_002078"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Met,seq:cat)
     gene            342..1439
                     /gene="mltA"
                     /locus_tag="LAF92_002079"
     CDS             342..1439
                     /gene="mltA"
                     /locus_tag="LAF92_002079"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBI9791086.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1516..2322
                     /gene="tcdA"
                     /locus_tag="LAF92_002080"
     CDS             1516..2322
                     /gene="tcdA"
                     /locus_tag="LAF92_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBI9791087.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2373..2816)
                     /gene="csdE"
                     /locus_tag="LAF92_002081"
     CDS             complement(2373..2816)
                     /gene="csdE"
                     /locus_tag="LAF92_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBI9791088.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2816..4021)
                     /gene="csdA"
                     /locus_tag="LAF92_002082"
     CDS             complement(2816..4021)
                     /gene="csdA"
                     /locus_tag="LAF92_002082"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBI9791089.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSALLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQYCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4213..4440
                     /locus_tag="LAF92_002083"
     CDS             4213..4440
                     /locus_tag="LAF92_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBI9791090.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4791..5708
                     /gene="gcvA"
                     /locus_tag="LAF92_002084"
     CDS             4791..5708
                     /gene="gcvA"
                     /locus_tag="LAF92_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBI9791091.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5727..6122
                     /locus_tag="LAF92_002085"
     CDS             5727..6122
                     /locus_tag="LAF92_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBI9791092.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6115..7215
                     /gene="rlmM"
                     /locus_tag="LAF92_002086"
     CDS             6115..7215
                     /gene="rlmM"
                     /locus_tag="LAF92_002086"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBI9791093.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7259..7990)
                     /gene="fucR"
                     /locus_tag="LAF92_002087"
     CDS             complement(7259..7990)
                     /gene="fucR"
                     /locus_tag="LAF92_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBI9791094.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8048..8470)
                     /gene="fucU"
                     /locus_tag="LAF92_002088"
     CDS             complement(8048..8470)
                     /gene="fucU"
                     /locus_tag="LAF92_002088"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBI9791095.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8472..9890)
                     /gene="fucK"
                     /locus_tag="LAF92_002089"
     CDS             complement(8472..9890)
                     /gene="fucK"
                     /locus_tag="LAF92_002089"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBI9791096.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9999..11774)
                     /gene="fucI"
                     /locus_tag="LAF92_002090"
     CDS             complement(9999..11774)
                     /gene="fucI"
                     /locus_tag="LAF92_002090"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBI9791097.1"
                     /translation="MKINTLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            12244..13743
                     /locus_tag="LAF92_002091"
     CDS             12244..13743
                     /locus_tag="LAF92_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI9791098.1"
                     /translation="MSETFLQMKHITKRFPGVLALNDVQFTLRRGEVHALLGENGAGK
                     STLMKILSGVYQPDEGEIIFEDKPVSFSDPLSAQNVGITIIHQEFNLFPELTVEENIF
                     IGREFCKKNRWRLDEKQQRQATIEILQKLNLAIKPDTLVADLTVAQQQMVEIAKAISV
                     NARILIMDEPTAALTETEIESLFRVTRLLKEQGTGIVYISHRLEELALIADRATVMRD
                     GQYISTVDYECVKISDLIAMMVGRDLGNIYPRREALQQRIPVLEVNGLTRKGVLNDIN
                     FTLYRGEILGFAGLMGAGRTELARAIFGADSIDSGTLKLNGKETVIKDISDAIQQGIS
                     YLTEDRKKEGLALNLSVERNIMLGNYPEYSDRFGNIDSRRCQQTSEEQVKALRIKTPN
                     LEQAALNLSGGNQQKIIIARWVCKDTDILIFDEPTRGIDVGAKLEIYELMNRLVAKGK
                     SIIMISSELPEVLGMCDRILVMRSGRITGELSAKEATQEKIMQYATLED"
     gene            13747..14730
                     /locus_tag="LAF92_002092"
     CDS             13747..14730
                     /locus_tag="LAF92_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9791099.1"
                     /translation="MTISVTSPDSNNKKIKINKELLMRLAPLFSLIILVLFFSFSSPF
                     FFNTENIMTIALQTSVIGIMAIGVTFVIITAGIDLSLGSVVAFSGVAVGICATLGLPL
                     PVCIIAGVLAGGLCGYVNGLLVTKMTIPPFIATLGLMMSVRGINMVMTDGRAIYFADY
                     PMFKTLAQGRLFDVLPYPVFYLVIVALVGAYILKKTVIGRYVYAVGSNEVAAHLSGIK
                     VQRVKIFVYAFCGLLTGIAGVILASRLNSGQPTVGVGYELEAIAAVVIGGTSLMGGIG
                     TIGGTIIGAFIMSVLKNGLNLMGVSQFWQMVAMGVVVVAAVYLDTLRKKIR"
     gene            14804..15754
                     /locus_tag="LAF92_002093"
     CDS             14804..15754
                     /locus_tag="LAF92_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI9791100.1"
                     /translation="MKTRHFIYALSLLACVTSSALAKDLNLPVVSKGFQHEFWQTVKM
                     GTEAAAKELGDKTSYVGPADETQIAEQIQLVENAMAQKPNGLLLAALDANALAPLVET
                     ANSRGIKVVTFDSGINSDIPVSFVATNNRKAGAQAADALAAQVNNKGKVGIIAHVAGT
                     SSAIERSEGFMERMKEKYPDIKVLPVQYSDGDPQKAMDKTIDMIQANPDLAGIYGTNE
                     GSTLGVANAIDSQNLKGKVKVIGFDSTEAIINFLKNGVIQGFVVQDAYQIGYQGIKTL
                     NAALSGQAVEKEIDIPVKFVNAENINTPEIDKLLHPFGKK"
     gene            15807..17387
                     /locus_tag="LAF92_002094"
     CDS             15807..17387
                     /locus_tag="LAF92_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBI9791101.1"
                     /translation="MHNGYFLGVDVGSASVRAGVYNASGHRLAFATAPVSQFRPGGER
                     VEQSSAEIWQQVCKTVKEATALAGIPISAIRSLGFDATCSLVVLDEQGQGLAVSSGGS
                     ANHDIIMWMDHRATVETAQINATKDPVLRYVGGEVSVEMELPKLRWLKTHLPQTWQAA
                     HRFFDLADFLVWKATGRDVAGLCTLTCKWNYLAHEQRFSHSLLESVDLTDMLERIPAE
                     ILPPGAAVGTLTAKAAEELGLTTNVIVASGLIDAHAGGMALAGAHPSGTLALISGTSN
                     CHMLCSEKEIFTPGVWGPYWSAMLPNYWLTEGGQSAAGALVEWTLQESGASANLFHKA
                     QQRGCHPIQLINEWVAALEANESEPTRNLHVLADHHGNRSPRARPDARGSVYGLTLER
                     GENQLARLYLATLQAIAFGTRHIIETLRENGHTITRLTLCGGATHNPLWLREYADVTG
                     CDIHLMQEEDAVTLGAAISGAVASGAWGDFTSACKAMVEAGEVIQVNPQRREFLERKY
                     RVYLTLWEQQQAVNQLMQ"
     gene            17452..18450
                     /locus_tag="LAF92_002095"
     CDS             17452..18450
                     /locus_tag="LAF92_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI9791102.1"
                     /translation="MAKTVEQIASDLNLSVTTVRLVLNGKAEQYRISVKTQTRINEYV
                     ERYGYIINHSARSLKLNKTDTLGLIVPNISNVFFATLAEKLEQRCRRSGYQLTISCTY
                     DDVDYENKLTKAFIARNVDGLFIVPSTLENQQHHLRKIRKPMVLLDRDFKYTDNALVE
                     SNNISGGEKLTQNILEAGQAPVWFLLGDAGLPSISDRLTGYLNALEKKGITHRDWVRE
                     GPVNTPEGGYVIMEKLIEEQGCPQAFIASSLPVLEGAVRAIRDRLGAVPPEINIGTFD
                     EHPMLGFLANNVWSMQQDENAWAEKAFEMMLSAIEERPVKKTVKVEMKLIKRIRKK"
     gene            18583..19230
                     /gene="fucA"
                     /locus_tag="LAF92_002096"
     CDS             18583..19230
                     /gene="fucA"
                     /locus_tag="LAF92_002096"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBI9791103.1"
                     /translation="MERTRLSREIIETCLEMTRLGLNQGTAGNVSTRFENGMLITPSG
                     IPYERMTENMIVYVDGNGKYEEGKIPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            19258..20406
                     /gene="fucO"
                     /locus_tag="LAF92_002097"
     CDS             19258..20406
                     /gene="fucO"
                     /locus_tag="LAF92_002097"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBI9791104.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(20461..21216)
                     /gene="xni"
                     /locus_tag="LAF92_002098"
     CDS             complement(20461..21216)
                     /gene="xni"
                     /locus_tag="LAF92_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBI9791105.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(21328..22695)
                     /gene="sdaB"
                     /locus_tag="LAF92_002099"
     CDS             complement(21328..22695)
                     /gene="sdaB"
                     /locus_tag="LAF92_002099"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBI9791106.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(22753..24042)
                     /gene="sdaC"
                     /locus_tag="LAF92_002100"
     CDS             complement(22753..24042)
                     /gene="sdaC"
                     /locus_tag="LAF92_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBI9791107.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(24599..25963)
                     /gene="ppnN"
                     /locus_tag="LAF92_002101"
                     /gene_synonym="ygdH"
     CDS             complement(24599..25963)
                     /gene="ppnN"
                     /locus_tag="LAF92_002101"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBI9791108.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(26075..26923)
                     /gene="queF"
                     /locus_tag="LAF92_002102"
     CDS             complement(26075..26923)
                     /gene="queF"
                     /locus_tag="LAF92_002102"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBI9791109.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            26991..27536
                     /gene="syd"
                     /locus_tag="LAF92_002103"
     CDS             26991..27536
                     /gene="syd"
                     /locus_tag="LAF92_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBI9791110.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            28158..28487
                     /locus_tag="LAF92_002104"
     CDS             28158..28487
                     /locus_tag="LAF92_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBI9791111.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            28487..29269
                     /gene="truC"
                     /locus_tag="LAF92_002105"
     CDS             28487..29269
                     /gene="truC"
                     /locus_tag="LAF92_002105"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBI9791112.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            29287..29736
                     /locus_tag="LAF92_002106"
     CDS             29287..29736
                     /locus_tag="LAF92_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBI9791113.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            30171..31523
                     /gene="gudP"
                     /locus_tag="LAF92_002107"
     CDS             30171..31523
                     /gene="gudP"
                     /locus_tag="LAF92_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBI9791114.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
BASE COUNT         7828 a         7523 c         8309 g         7906 t
ORIGIN      
        1 ctaccaggct gctccacccc gcgtcaccgt actgctttac ttcatcaaat tttaattggt
       61 tgcgggggcc ggatttgaac cgacgacctt cgggttatga gcccgacgag ctaccaggct
      121 gctccacccc gcgtccgtgg atgcgcacta tactccgctc gcgttttcat gcaacctttt
      181 ttttaccccg atcacggatt tagcatgatt ttgaacaaaa acaaaccact aaagcctatt
      241 ttttgtgcaa aatttgcgtc agggagaggc tgctcatttc attaaacgcg gcggtttgtt
      301 atcttcgttg cgccttattt tttaacctga agaagagaac aatgaaagga cgttgggtaa
      361 agtaccttct tatgggcacg gttgtggcaa tgcttgccgc ctgctcttcc aaaccaaccg
      421 atcgcggaca gcaatataaa gacgggaaat ttacccagcc tttctctctg gtgaaccagc
      481 cagatgccgt tggcgcgccg attaacgccg gtgattttgc cgagcaaatt aaccatatcc
      541 gtaattcgtc accgcgtctg tatggcaacc agagtaatgt ttataacgcg gtgcaagagt
      601 ggctgcgcgc aggcggtgat acccgcaata tgcgccagtt cggcattgat gcctggcaga
      661 tggaaggtgc cgacaactat ggtaacgtgc agtttacagg ctattacacg ccggtaattc
      721 aggcgcgcca tacccgccag ggcgagttcc agtatcctat ttaccgtatg ccgccaaaac
      781 gtggtcgtct gccgtctcgt gcggagatct acgcaggggc gctgagtgat aaatatattc
      841 tcgcttacag caactccctg atggataact tcattatgga tgtgcagggt agcgggtata
      901 tcgactttgg tgatggcagt ccgcttaact ttttcagcta tgcagggaaa aacggtcatg
      961 cctatcgcag cattggtaag gtgctgatcg accgtggcga agtgaaaaaa gaagatatgt
     1021 cgatgcaggc gattcgtcat tggggcgaaa cacacagtga agccgaggtt cgcgagctgc
     1081 tggaacagaa cccgtctttc gttttcttta aaccgcaatc ttttgcaccg gtgaaagggg
     1141 caagtgcggt gccgctggtt ggccgtgctt ctgttgcctc cgatcgttca attattccgc
     1201 caggtactac cttgctggca gaagtgccgc tgctggataa taacggcaaa tttaacggtc
     1261 agtacgaatt gcgtctgatg gtggcgctgg atgtcggtgg cgcaatcaaa ggccaacact
     1321 tcgatatcta tcaagggatc gggccggaag ccggacatcg cgcaggttgg tacaaccact
     1381 atggacgtgt ctgggtgctg aaaaccgccc cgggcgcagg taacgtcttt agcggctgat
     1441 gtgttattct gtataccgtt tttacggata cgccagggtg agggataacc tcaccctttt
     1501 taattctgag gtgctatgtc tgtggtaatt agtgatgcat ggcgtcagcg ttttggtggc
     1561 acagcgcgtc tgtatggtga aaaagcgttg caactgtttg ctgacgcgca tatttgtgtg
     1621 gttggtatcg gtggtgtcgg ttcctgggcg gcggaagcgc tggcgcgcac ggggattggc
     1681 gcaatcacgc ttatcgatat ggatgatgtg tgcgtcacca ataccaatcg gcaaattcac
     1741 gctctgcgcg ataacgtcgg gctggcaaaa gcggaagtca tggcggagcg tattcgccag
     1801 attaacccgg agtgccgtgt aacggtggtg gatgatttcg tgacgccgga taacgtagcg
     1861 cagtatatga gcgtgggtta ttcgtacgtg attgatgcca ttgatagtgt acggcccaaa
     1921 gcggcgctga ttgcttattg tcggcgcaat aaaatcccgc tggtcacgac cggtggcgcg
     1981 ggtgggcaga ttgacccgac gcagattcag gttaccgatc tggcgaaaac gattcaggat
     2041 ccgctggcgg cgaagttgcg cgagcgcctg aaaagcgatt ttggcgtagt gaaaaacagt
     2101 aaaggtaagc tcggcgtcga ttgcgtgttt tctactgaag cgctggtgta cccgcagtca
     2161 gacggtactg tgtgtgcgat gaaagccacg gcagaagggc cgaagcggat ggattgtgca
     2221 tctggatttg gcgcggcaac gatggtgacc gccacctttg ggtttgttgc ggtttctcat
     2281 gcgctgaaga agatgatggc gaaagcggcg cgtcagggtt aaacaatcag gtcggatgcg
     2341 acgcgaacgt cttatccgac ctggttctgt gcttaaacct gcttcgccgc agcgataatc
     2401 gcctcgctta acgcattcaa cccctggctg cgtgaggcgc taagctgtgc acgcaatccc
     2461 agctcatcaa acaatgccag cggcgactgt gcctgcaatt cggcgacggt tttcccctca
     2521 acggcagtca acaacaccgc caacaggccg cgcacaatgc gcccttcgct gtcgccaaag
     2581 aagtgcattt taccgttttc agtcacggta tatcccagcc agacgcggtt ttcgcatccg
     2641 gcaatctctt tagcctgcgc ttttaactcg tctggcaaag ccggaagctg tttccccagc
     2701 atgatcaact ggcgatattt atcttcccat tgcgacaacg gtgcgaaggt attgcgtaac
     2761 gtttctgcgg ttacggttgt gccgaacgga tgtccggcga attgcgggtt tgtcattaat
     2821 ccaccaataa ttccagcgcg cggtcaacgg cattcaccag cgcatccaca tcactctttg
     2881 tattatatgg cgcaaaagag gcgcgcagtg tgccggttac gcctaattct gccagtagcg
     2941 gctgagcgca atactgcccg gcccgcaggg caataccgta ctccgccagc agcgtcacca
     3001 tatcgctatg atgaacgcca gcaaaatcaa aggccagcag gctggaatcc tggcagcgga
     3061 atgaacgaaa gccgggacgt ttcgccagcg catcttccgc cagcgttgct aagctacggc
     3121 tccagctttc ggcctggttg atatcgtaat ctgccagcca ttccagcgcc gcgcttaatc
     3181 ctatgacacc agcgacattt ggcgttccag cttccagttt ccacggcgca gattgagtcg
     3241 tgaagccgtc aaaactcact tcgtgaacca ttttgccgcc gcccagccag ggcgacatcg
     3301 cctccagcag tgctgattta ccatacagca cgccgatacc tgtcgggcca tacagtttgt
     3361 gacctgaaaa agcatagaaa tcaatatcca gttgctgaac atccgcgggg aaatgcactg
     3421 ccccctgagc accatcaacc atcaccacca tcccggctga atgagcaaag gtaatcgctc
     3481 gcgccagatc cgggcaaccg ccagtaacgt tcgacatctg acccaacgcc agaatccgac
     3541 tacggggagt aatcagttct ggcaacaaat cgacatccgg cagtcgctgc gcattaagcg
     3601 gcaatttcac cactttggct ccagtttgtt gggcgaccat cagccagggg acgaggttgg
     3661 cgtggtgttc tgccacgctg acaataatct catcgcccgg ttgcagacgc ggacgcgcat
     3721 agcattgtgc caccatgttg atggattcag tggtgccgcg cgtccagacg atagttttat
     3781 catccggtgc attcagtaat tgcgccactt tctctcgtgc agcttcataa cgcgcggtca
     3841 ggcgttgggc ttcggcaaac tggctgcgat ggacgtttcc ggcgctcaga ctgtaaaact
     3901 gttgggtggc ttcaaccacg gcttcaggtt taagcgcggt cgcggcgctg tcgagataga
     3961 cgcccgcatc ctgtagtgcg ggaaactggg cgcgaaactg cgcgggatta aaaacgttca
     4021 tggtactcct cggcttgatg caccgatcgt gacgcaaaat tcactttgat acaaggagag
     4081 tgctaaccat cagaattatc tggatatcct ggcgaagccg cgcaagtaac atatgctgaa
     4141 tagtgttagg cgttaatttt agcctgttaa tcaatactat taagatttat taatcgcttt
     4201 aaggagaaga tgatgaaaaa gactgccgca attatttctg cctgtatgct gacttttgcc
     4261 ctgagcgcct gttccggttc gaactatgtg atgcacacca atgacggacg taccatcgtc
     4321 tctgacggca aaccacagac tgataacgat accggtatga tttcgtataa agacgctaat
     4381 ggcaacaaac agcagatcaa ccgtactgac gtgaaagaga tggtcgaact ggatcagtaa
     4441 ttcgcgatcg caaggtaaaa aaaagcaccg caattaggcg gtgctacatt aatcactatg
     4501 gacagacagg gtaaatgtac aggaagtgaa aaaaggtagc tttgctacca tggtctgaat
     4561 cgcagaccaa ttgcaaacac aacaacacaa catcacaacc gtaagccaaa agttcaccag
     4621 aacacgcatt ccgataaaac ttttcgttcc ggctcaggaa gtgccgccac tataggtatt
     4681 tgctggtaga agctcaacgg acaatttata atggctcaga ttaaaaaaac taataggtta
     4741 cacagtgtga tctaattgtt aaattcattt aacatcaaag tttaaaagcc atgtctaaac
     4801 gattaccacc gctaaatgcc ttacgagttt ttgatgccgc agcacgccat ttaagtttca
     4861 ctcgcgcagc agaagagctt tttgtgaccc aggccgcagt aagtcatcaa atcaagtctc
     4921 ttgaggattt tttggggcta aaactgttcc gccgccgtaa tcgttcactc ctgctgaccg
     4981 aggaagggca aagctatttc ctcgatatca aagagatatt ttcgcaatta accgaagcga
     5041 cgcgtaaact ccaggcccgt agcgccaagg gggcgttgac ggtcagttta ctccccagtt
     5101 tcgccattca ttggttggtt ccgcgacttt ccagctttaa ttcagcttat ccgggaattg
     5161 acgttcgaat ccaggcggtt gatcgtcagg aagataagct ggcggatgat gttgatgtgg
     5221 cgatatttta tggtcggggc aactggccgg ggctacgggt ggaaaaactg tacgccgaat
     5281 atttattgcc ggtgtgttcg ccgctactgc tgacaggcga aaaacccttg aagaccccgg
     5341 aagatctggc taaacatacg ttattacatg atgcgtcacg ccgtgactgg cagacatata
     5401 cccgacagtt ggggttaaat catatcaacg ttcagcaagg gccaattttt agtcatagcg
     5461 ccatggtgct gcaagcggct attcacgggc agggagtggc gttggcaaat aacgtgatgg
     5521 cgcaatctga aatcgaggcc ggacgtcttg tttgcccgtt taatgatgtt ctggtcagta
     5581 aaaacgcttt ttatctggtt tgtcatgaca gccaggcaga actgggtaaa atagccgcct
     5641 ttcgccaatg gatcctggcg aaagccgctg ctgaacaaga aaaattccgc tttcgttatg
     5701 aacaataatt tacgtagggt acgaccatga ccagccgttt catgctgatt ttcgccgcca
     5761 ttagcggctt catttttgtg gctctgggcg cttttggcgc gcatgtgtta agtaaaacca
     5821 tgggggccgt tgagatgggc tggatccaga ccggcctcga ataccaggcg tttcatacgc
     5881 tggcgatctt aggtctggcg gtggcaatgc agcgtcgcat cagtatctgg ttttactgga
     5941 gtagcgtttt cctcgcgtta ggcacggtgt tgttcagcgg cagcctttat tgcctggcgc
     6001 tgtcccatct gcgtttgtgg gcgtttgtca ctccggttgg cggcgtgagc ttcctcgcgg
     6061 gctgggcgtt aatgttagtt ggtgctatcc gtttaaagcg caagggcgta agtcatgaat
     6121 aaggttgtat tgctgtgccg tccgggcttt gaaaaagagt gcgccgcaga aattaccgat
     6181 aaagccggcc agcgggaaat tttcggtttt gcccgcgtga aagagaatgc gggttatgtc
     6241 atttatgaat gttatcaacc tgatgatggc gataagttaa tccgtgagct gccgttcagt
     6301 tcattaattt ttgcccgcca gtggtttgtg gtgggggaac tcctgcagca tttgccgcca
     6361 gaagatcgta ttacccccat tgtcggcatg ttacagggcg tagtagagaa gggcggtgaa
     6421 ctgcgtgttg aagttgccga taccaacgaa agcaaagagt tactgaaatt ctgccgtaaa
     6481 tttactgttc cgctacgcgc tgccttgcgc gatgccgggg tgctggcgaa ctatgaaacg
     6541 ccgaagcgtc cggttgtgca tgtattcttt attgcaccag gctgctgcta taccggttac
     6601 tcatacagca acaataattc gccgttctat atgggcattc cgcgcctgaa atttccggca
     6661 gatgcgccga gtcgttccac gctcaaactg gaagaggcat ttcatgtgtt tattcctgcg
     6721 gatgagtggg atgaacgcct ggcgaacggg atgtgggcgg tggatttagg cgcttgccct
     6781 ggcggctgga cctaccaact ggtgaagcgc aacatgtggg tttattccgt cgacaacggc
     6841 ccgatggcgc aaagtctgat ggataccgga caggtgacgt ggctgcggga agacggtttc
     6901 aaattccgtc cgacgcgcag caatatctcc tggatggtat gcgatatggt tgaaaaaccg
     6961 gcgaaagttg cggcattgat ggcgcagtgg ctggttaatg gctggtgccg tgaaaccatt
     7021 ttcaacctca aactgccgat gaaaaaacgc tacgaagaag tgtcacacaa tctggcgtat
     7081 attcaggcac agcttgatga acatggcata aatgctcaga ttcaggcgcg gcagttgtat
     7141 cacgatcgcg aagaagtgac ggtgcacgtc cgccgcatct gggctgcggt gggtggtcgt
     7201 cgcgacgagc gataacaggc aaggccgggg cgtcgtctcc ggcctgctac cctttccgtc
     7261 aggctgttac caaagaagtt gcaacctggc gctcatcaga aataatgtgc gttacctcat
     7321 ccagatgccc gatgcgggct tcccctgaac gattaaattt acttttatca atcaataaca
     7381 acgattgcgc ggcacgtttt aatagcatcg atttgtaatc agcgttgatc gcattggagt
     7441 cccacagtgc gccgctgcta tcgatccctt cacaagaaaa aataaacaga tcgatttcca
     7501 gcgatttaag ttgggaaatc agcgagggat tgacgtaaca gccatatttg cgctcaagtg
     7561 tgccgccgga actgatcagt tgaatgcgtt cgcgtttacc gagttcatgg caaatcggat
     7621 ggctattggt gaagacctga atgttgatgt caggcaactg gcgtgccaga taccagcaag
     7681 ttgaactggc gtctaaggct atcaccatcc cttcttcaat ccacgcgagc gcctcgcgcg
     7741 cgatatctgc tttatgcgca taatggcttt tcagccggat gtgaaaggga tcgccactgt
     7801 cttgattttg acggtggata tatttagcgc gtccatgatt gcgcagaatt ttaccctgcg
     7861 tctgtaattc attgagatcg cgacgaatgg tttctttact gacctttagc tgttcagaga
     7921 gagcttccgt ggtcaggctg gtatggttca gcagcaggtc gactatcgct tgctggcgtg
     7981 ccgctttcat atttttcacc ccatcattcg ataaccgccc gcgtcatgcg ggcgtaccgg
     8041 tatgagatta cggtgttacc ccttttttta aaagaatatt cccgtacttc gctcgttcgc
     8101 ctgtgataac gatcgcaaag gctttttgcg cccgttcata aaacgcaaaa cgattgatgc
     8161 ggatgatgtc aggacacggg gcttgtagtg aaagcgcatt acggtaacgt cgttctactt
     8221 caggatcgag agtgtcacct tctaccgccg ccatcatcac cagcggcggt gcataactgt
     8281 ccagttcaaa taacgggata atcgcctgga gcaagtcgct caccaacagg ccatcagcgc
     8341 ggatcacctg cggtcccatc gaatgggcgg gaaagtgagc atcggaaaaa ataatttcat
     8401 ctccatgtcc catctctgcc agcactttca atagttcggg agaaattaac ggcgaaattg
     8461 ttttcagcat ttcacacttc ctctataaat tcaggttcag tttgcgggta gaaataacgg
     8521 tactgataat gaatctgtgc gcgggcttct tccgggctgt taaattcccc tacgccatac
     8581 caaccgaaca gcgcagctcc tgcgacggtc gtttcggcgt cgtcgagaac ttttaccgga
     8641 atatcaagca tattggcttt aatctgattc cacaatgtgt tgcgacttcc tccgccgact
     8701 aacaataatt cagaggcttt aaagtgcccg attttttcca gcatctgtag attgcgctgt
     8761 aattgcgcgg ttaacccttc cagcgccgcg cgatagaaat gcccccgcgt ggtattaagc
     8821 gtcactcctt gccagccagc gttctgacac gacaataaat cacactgcat ttttacgcca
     8881 tccgcgccag gcgcgatcag acgagcctct tcaatcaaca tttgccaggg tgtttcagcc
     8941 gtccagaaca gttttctcac ccattccagc acgccggatg ccagccattg catacctggg
     9001 ttatacaacc ctgcctggct atccagttcg caggtggaac cggcgtactg acttaacagc
     9061 gaagtatcaa cctgggcgct acggaccatt aaaatttccc atgtaccgga agagagcacg
     9121 ggttcatttt ggtcggcacc agcgccaaaa agggcgaact gggtatcgtg acctgcggaa
     9181 atcaccggta tgccaacggg taagccgagc attgctgcgg cgctgttctg tagcgtacca
     9241 atctgttcac ccgcttccac cagacgaggg aagagtcggc gtggaatacc ggtggcttgt
     9301 aaaatttgcg gactgaaatc gcgttgctgg atatccagca tctggctggt tccggccatc
     9361 gtgatatcag tagtgaattc gccggttaaa cggtggttaa tcagcgacga aataaagagc
     9421 caggcgtgcg cgcgttccag cagttgtgga tgattttctt tcaaccacac caacttatat
     9481 aacgtattga aactaaaggc tccgacgcca gaaatagcct gcaaccgctg tgcggagatc
     9541 aactgttcaa tattgtccat aaccgctgct gttcgcggac atttccagct aatgatcgga
     9601 tagagcagat tgccttgctt atctaccaga gcgccatcca caccaaaggt ggtgacggcg
     9661 ataccgcgga tgtggcattc agtcagttca ctattgattt gccgacagca atcagcaaag
     9721 cgttgcaaaa tggcgtctaa agaccactgg tgccaggtgt tgttttccat cgcgatatcg
     9781 ctggcattag gcgttgaggc gcgggcaaca atttttccct gccgattaac cgcgatggcc
     9841 ctgacattgg tcgcgccaca gtcgagtacc aggataactt cttgtttcat aatcgctcct
     9901 gcaatatagc cggataacat tgcttatccg gctaaccact cttgcttgtt caggcccggt
     9961 agccggagcg accgggcatc acatcaggga gtaatgtatt aacgcttgta caacgggccg
    10021 tagttctggc aagcgcggta atcctgacct tcaatatcca tgccgtgcgc ggcccaggca
    10081 gaaggacgat atactttggt ctcttcaacg ttgtgcatac ataccgggat acgcagcatg
    10141 gaggcgagag tgataaagtc tgcgccaacg tggccgatgg tcagaacccc gtggttagcg
    10201 ccccagttcg ccattaccga atacacatcc gtaaacggcc ctttaccggt gaggcgcggt
    10261 gcaaaccagg tggttggcca ggttgagttg gtgcgtttgt tgaggatgtc atgcacatcc
    10321 ttcggcaatt ccacgctcca gccttccgcg atttgcagta ccggtcccag gcctttgatg
    10381 atgttgacac gagtcatggt gaacgggacg ccgccttcgg taaggaagcg ggaagagtaa
    10441 ccgccgccac ggaagtattc gtggatcgcc gggcaccatt cggtagcggc gaggcaagcg
    10501 tcagcctctt gctgagagat ttcccagtgt ggcttcatcg tcgggttacc ttcgctgtcg
    10561 cgttgtttac aggaaccgtc cagcgcagca gaaccggagt tgatcaaatg gatgatgccg
    10621 tgttctgcca gtccatcgag tttatgcccc gttacacgct caattgcttc tggtgaccag
    10681 taggtacgca catcggcaaa tacctgagcg gtgccggtga gctggtgacc cattagcatt
    10741 gccacgccgt taagactgtc gttttcggtc gccacgacaa agggttcgcg cacgccattc
    10801 cagtcaaatg aactgttgag gatcgcttcg gcggtgtcac cattgggata ttgatcggtc
    10861 cagtgacgtt gcccctggaa gcccgcagcg atggcgttgt agccaagtga ttcttccacg
    10921 cgaccaatat cggccagttt gctgttgcct tgcatcatgt cgcggataca catcgccatc
    10981 agtaaacttt cgcgcagaac tgcgcggctt tgctcggcat tacgttgata ttgtttgtta
    11041 ttttcatctt cgccatagcg gaagttttta tcagcccagg ccagcgccat ttccagttcg
    11101 gcttcgtcgt aaatcttctg atcgatacgg cgacgcagtt cggtcatatc caccgcctgg
    11161 actttcattc ccagccagga ttcaaagaag ttgtgatcaa caatagaacc ggcgataccc
    11221 atcgaaacgc cgcccagcga cagatagctt ttacctttca tgctggcgac ggccaaaccg
    11281 gcgcgggcaa agcgcagcag tttttcttca acatcggcag gaatcgatgt gtcatcggca
    11341 tcctgaacgt catgaccgta aatggagaat gccgggatgc ctttctggct gtgagctgcc
    11401 agagccgctg ccaggtaaac agcgccggga cgttcagtgc cgttaaagcc ccaaatggct
    11461 ttcgggcggg ttggatccat gtcgatggtt tcactgccat agcaccagca aggcgttacc
    11521 gtaatggtga ggcctacatt ctgactgctg aatttttctt cgcaagcagc ggcttcagcc
    11581 atacccgcga tacaggtatc ggaaatgaca cactcgacgg cagctccgca ggcatggcgc
    11641 agtttctcgg tcagcagtgc ggccgtagct ttcgccatat tcattgtttg ttcttcaagc
    11701 gactcacgaa cacccatgcg acgaccgtca ataaccgggc ggataccaat tttcggtaac
    11761 gtattaattt tcatcaggat tcctcatctc gctttattgg cgctctgcta aggcggagaa
    11821 aggaaaccac cgaagcagta gtattggtta ttatttgcta actcgagtta gcttaggttt
    11881 gtaggctttt tatgcacaac ttttttgttg ttgaaatgtg aggggcttca cgagaagtgg
    11941 ttaaaatacg atctctgtca aattattatt tagcgggata aaaataaatt ttacgtataa
    12001 tcatatggtt attagatttt aatggttatg catcacagtt ttaactcaaa tgatttggct
    12061 aatttgtata cctaaaatag ctttaaaagg cgatcgctaa ctcgagttag caaaatgcga
    12121 agaatagatt gatgcgatga atatttattt aaaggttatt cgtatcacag tggagaaggc
    12181 tttagtagcg acgtttatgc cgtacttcac attttcagtt tctgattatt gaggaaagct
    12241 gcaatgtccg aaacattttt gcaaatgaaa catatcacca aacgctttcc tggagtgttg
    12301 gcgcttaatg acgtgcaatt taccctacgc cggggcgagg ttcatgcatt attaggtgaa
    12361 aatggtgcag gaaaatctac cttaatgaaa attttgtcag gggtttatca acctgatgaa
    12421 ggcgaaataa tttttgaaga taaaccagtc tccttttctg atccgttaag tgcgcaaaac
    12481 gtaggtatta ctattattca ccaggaattt aatttatttc ccgagcttac agtagaggaa
    12541 aatattttta tcggcaggga gttttgtaaa aaaaatcgct ggcgtctgga tgaaaaacag
    12601 caacgccagg cgacgattga aattttgcaa aaattaaatt tggcgatcaa accagatact
    12661 ctggttgccg accttactgt agctcagcag cagatggtag aaattgccaa ggccatttca
    12721 gttaacgcca ggattctcat tatggatgaa cctactgcgg cgttaacaga aactgaaatt
    12781 gaaagcttat ttcgcgtcac ccggttattg aaagagcagg ggaccggcat tgtgtatatc
    12841 tcccaccgtc tggaagagtt ggcgctaatt gccgaccgtg cgactgtaat gcgtgatggt
    12901 cagtacatca gcacggtgga ttatgagtgc gtaaaaatca gcgatctgat tgccatgatg
    12961 gttggtcgcg atttagggaa tatctatccc cgccgcgagg ccttgcagca gcggattccg
    13021 gttctggagg taaacggact gacgcgcaaa ggcgtcctga acgatattaa ttttacgctt
    13081 tatcgtggcg agatcctcgg ctttgccgga ttgatgggcg cggggcgtac tgaacttgcg
    13141 cgtgcgattt ttggtgcgga ttctattgac agcggaacgc taaaattaaa tggtaaggaa
    13201 accgttatta aagatatttc ggatgctatt cagcagggaa ttagttacct gacggaagac
    13261 cgcaaaaaag aagggctggc gctgaattta tctgttgaac gaaatattat gttaggaaac
    13321 taccctgagt attccgatcg ttttgggaat atcgattcgc gtcgttgcca acaaaccagt
    13381 gaggaacagg taaaagcgtt aaggattaaa acgccaaatc ttgaacaggc ggcattaaat
    13441 ctaagtggag gaaaccagca gaaaattata attgcacgtt gggtgtgtaa agatactgat
    13501 attttaatct tcgatgaacc cactcgcggc attgatgttg gcgccaaact ggaaatctat
    13561 gagttaatga atcgcctggt ggcaaaaggg aaatcaatca tcatgatctc ctctgaattg
    13621 ccggaggtgt taggaatgtg cgaccgtatt ttagtgatgc gcagtggtcg aattaccggc
    13681 gaacttagcg caaaagaagc gacacaagaa aaaattatgc aatacgcgac gttagaggat
    13741 taaattatga ctatctctgt aacctctcca gacagtaata ataagaaaat aaagataaat
    13801 aaagaacttt tgatgcgcct tgcacctctt tttagcttaa ttatattggt gctatttttt
    13861 agtttcagct ctcccttttt ctttaatacc gaaaatatca tgacgattgc tctacaaacg
    13921 tcggtaatcg gcattatggc cattggggta actttcgtca ttattaccgc tggcatcgat
    13981 ctttcattgg gctccgtggt ggctttttct ggtgtcgccg taggtatttg cgccacgctg
    14041 ggcctgccgc tgcctgtgtg tattattgct ggtgttttgg ccggtggatt gtgcggctat
    14101 gtgaatggtt tgttggtcac taaaatgacc attccaccgt ttatcgctac cttgggcctg
    14161 atgatgtccg tcagaggtat caatatggtg atgactgatg ggcgcgccat ctattttgcc
    14221 gattacccga tgttcaaaac gctcgctcag ggacgcttat ttgatgtgct gccttatcct
    14281 gttttctacc ttgtcattgt agcgcttgtc ggcgcttaca tcctgaagaa aaccgtcatt
    14341 ggtcgctatg tctatgccgt aggcagtaat gaagttgctg ctcatctttc tggtattaaa
    14401 gtccagcgcg tgaaaatttt cgtctatgcc ttctgtggat tattaacggg gattgcaggt
    14461 gtcattctgg catctcgtct caactccgga caaccgacgg taggtgtggg ttatgaactg
    14521 gaagctattg ccgcggtagt gattggcgga accagcttaa tgggtggtat tggcactatt
    14581 ggcggcacaa tcattggcgc tttcattatg agtgtactga aaaatggcct caatttaatg
    14641 ggcgtctccc agttctggca aatggtcgcg atgggcgtcg ttgtggtcgc tgccgtttac
    14701 ctcgatacat tacgcaaaaa gattcgttga cttaggctcc ctgttttatc gggaaacaac
    14761 tgccctgacg ttaagggtga ttcactcaga acggagtaca acaatgaaaa ccaggcattt
    14821 tatctatgcg ttatctctgc tggcctgcgt aacttccagc gcactggcga aggatttaaa
    14881 tcttccggtt gttagtaaag gtttccaaca tgaattctgg caaaccgtaa aaatggggac
    14941 tgaagctgcg gctaaagagt tgggggataa aaccagctat gttggcccgg ctgatgaaac
    15001 gcaaatagcc gagcaaattc agttagtcga aaatgcgatg gcgcagaaac cgaatggctt
    15061 gcttctggca gccctcgacg cgaatgcgct cgccccactg gtagaaacag caaattcacg
    15121 cggtatcaaa gtggtgacct ttgattcagg catcaattca gacattccgg tgagttttgt
    15181 cgccaccaat aaccgtaaag caggtgctca ggcagctgat gccttagctg cgcaggtaaa
    15241 taataaaggg aaagttggga ttatcgcgca cgttgcagga acatcttccg ctattgaacg
    15301 ctcggaaggc ttcatggagc gtatgaaaga aaaatatcct gacattaaag tcttacccgt
    15361 tcagtacagc gatggtgacc cgcaaaaagc gatggataaa actatcgata tgattcaggc
    15421 caatcccgat cttgctggga tctatggcac caatgaaggt tcaacgctcg gggtggcgaa
    15481 tgccattgat agccagaatc tgaaaggtaa ggtgaaggtc attggttttg atagtacgga
    15541 agcgattatt aacttcctga agaacggcgt cattcagggc tttgttgttc aggatgcata
    15601 ccagattggc tatcaaggga tcaaaacgtt gaacgcggcc ctttcggggc aagccgttga
    15661 aaaagagatc gatattcccg tgaaatttgt taatgccgag aatattaaca cgccagaaat
    15721 cgacaaactc ctgcacccat tcggtaagaa ataaaaaatt ggggatgaat tttcatcccc
    15781 gttatctcca gcgtcaggga ggctttatgc acaacggtta ttttctcggt gtcgatgtcg
    15841 gatcggccag tgtcagggca ggcgtttaca atgccagtgg ccacagactg gcattcgcta
    15901 ctgcgcccgt ctcccagttt cgccctggcg gcgagcgtgt cgaacagtct tcggcggaga
    15961 tctggcaaca agtttgtaaa acggtgaaag aggcgacagc tttggcaggc atccccattt
    16021 ccgcaattcg ctcactgggt tttgacgcta cctgctcgct ggttgttctt gatgaacaag
    16081 gccagggttt agcggtttcg tccggtggat ctgcgaatca cgacattatt atgtggatgg
    16141 atcatcgtgc aactgtcgag acggcgcaga ttaacgcaac gaaggatccc gtattgcgct
    16201 acgttggcgg cgaagtgagc gtcgagatgg agttaccaaa gttacgttgg ttgaagacgc
    16261 atctaccgca aacctggcag gccgctcatc gctttttcga tctggcggat tttctggtct
    16321 ggaaagcaac agggcgagac gtcgcgggat tgtgcacgct aacgtgtaaa tggaattatc
    16381 tggctcatga acaacgcttt agccatagct tactggaatc ggtagacctg accgacatgc
    16441 tcgaaagaat tcccgccgaa attcttccgc ctggcgctgc cgtgggaact ttaactgcca
    16501 aagctgctga agaattagga ttaacgacca atgttatcgt ggccagtggt ttgattgatg
    16561 cccatgccgg tggcatggcg ctggctggcg cgcacccatc aggaacgctg gcattgataa
    16621 gcggaacgtc gaattgccat atgttgtgca gtgaaaaaga aatattcacc cctggcgtct
    16681 gggggccata ctggtcggcc atgttgccaa attactggct aactgaaggc gggcaaagcg
    16741 ccgcaggggc gctggtggag tggacgcttc aggagtccgg ggcaagcgca aatctttttc
    16801 acaaggcgca gcaacgcggg tgtcatccga ttcaactgat taatgaatgg gtagccgccc
    16861 tggaagctaa cgagtcagaa cccacacgaa atttacatgt gcttgccgat catcacggca
    16921 accgttcgcc acgagcgcgg cccgatgcca gaggcagtgt ttatggctta acactggagc
    16981 gtggtgaaaa tcagctcgcg cggctttatc ttgcgacttt acaggcaata gcctttggca
    17041 ccagacatat tattgaaact ctcagggaaa atgggcatac cattacccga ttaacattat
    17101 gtggtggtgc aacgcacaac cctttatggc tacgcgaata tgcagacgtg acaggctgcg
    17161 atatccacct gatgcaagaa gaagatgccg ttacattggg tgcagcaatt agcggcgctg
    17221 ttgccagcgg agcatggggg gattttacat ctgcgtgtaa agcaatggtt gaagccggag
    17281 aggttattca ggtaaatcca caacgccgtg aatttttaga gcgtaaatac cgggtttacc
    17341 tgacgctttg ggagcaacaa caggcggtta accaactgat gcaataagac aaaagttgaa
    17401 aatactttta tgattgtgat ttgacgtctg gatgttaact gggaaatacc tatggctaaa
    17461 acagtagagc agatagccag cgacctgaat ttatcggtta cgaccgtgcg actggtgcta
    17521 aatggaaaag cggaacaata tcgaatcagc gtcaaaaccc aaacgcgtat caatgaatat
    17581 gttgaacgct atggttatat cattaatcat tccgctcgca gccttaagct taacaaaact
    17641 gatacattag ggttgattgt tcccaacata tccaacgtct tttttgctac gctggcagaa
    17701 aaactcgaac aacgctgtcg acgttccgga tatcaactta caatcagttg cacttatgat
    17761 gatgttgact atgaaaataa attgacgaaa gcatttatcg cacgaaatgt agatggtctt
    17821 tttattgttc cttcgacgct ggaaaatcag cagcatcatt tacgcaaaat tcgtaaacca
    17881 atggttttac tcgaccgtga ttttaaatat accgataatg cattggtaga aagtaataat
    17941 atttctggcg gagaaaagtt gacacaaaat attcttgagg ccgggcaggc tccagtatgg
    18001 tttttgctgg gggatgctgg tttgccgagt atcagcgatc gtctgacagg ttatttgaat
    18061 gcgttggaaa aaaaagggat tacccatcgc gattgggtgc gggaaggtcc ggtaaatact
    18121 ccggaaggcg gctatgtcat tatggaaaaa ctcattgaag agcagggatg tccgcaggca
    18181 tttatcgcct cgtctttacc tgtgcttgaa ggcgctgtcc gcgcgatacg cgaccgtctt
    18241 ggcgctgtac cgccagaaat caatattggc actttcgatg aacatcccat gctgggattt
    18301 ctcgccaaca atgtctggtc aatgcagcag gatgaaaatg cctgggcaga aaaagcgttc
    18361 gaaatgatgt taagcgcgat cgaagaaaga ccagtcaaaa aaacagttaa agtagaaatg
    18421 aaattaatta agcgcataag aaaaaaataa taatcgccaa aagatatcag gctactgaac
    18481 aatgatattc agtggcctcc ccttactaaa aataaccccg tcttcatttg cggtgctgtt
    18541 ttacgcaaca aggcgagctg aaactgtatc tggagaatag aaatggaaag aacacgttta
    18601 tcccgtgaaa taattgaaac ctgtctggaa atgacgcgcc ttgggttaaa tcaggggacc
    18661 gcggggaatg tcagcacacg ttttgaaaac ggcatgttga ttactcccag cggtattcct
    18721 tatgaaagaa tgacggagaa tatgattgtc tatgttgatg gcaacggcaa atatgaagaa
    18781 ggtaagattc cttccagcga atggcgtttc catatggcgg cctatcaaag cagaccggat
    18841 gccaacgcgg ttgttcacaa tcatgccgtt cattgcacgg cagtttccat tcttaaccga
    18901 tcgatccccg ctattcacta catgattgcg gcggctggcg gtaattctat tccttgcgcg
    18961 ccttatgcga cctttggaac acgtgaactt tctgaacatg ttgcgcaggc tctcaaaaat
    19021 cgtaaggcaa ctttgttaca acatcatggg cttatcgctt gtgaggtgaa tctggaaaaa
    19081 gcgttatggc tggcgcatga agttgaagtg ctggcgcaac tttacctgac gaccctggcg
    19141 attacggacc cggtgccagt gctgagcgat gaagagattg ccgtagtgct ggagaaattc
    19201 aaaacctatg ggttacggat tgaagagtaa tttcgtaaag caacaaggag aaggatgatg
    19261 gctaacagaa tgattctgaa cgaaacggca tggtttggtc ggggtgctgt tggggcttta
    19321 accgatgagg tgaaacgccg tggttatcag aaggcgctga tcgtcaccga taaaacgctg
    19381 gtgcaatgcg gcgtggtggc gaaagtgacc gataagatgg atgctgcagg gctggcatgg
    19441 gcgatttacg acggcgtagt gcccaaccca acaattactg tcgtcaaaga agggctcggt
    19501 gtattccaga atagcggcgc ggattacctg atcgctattg gtggtggttc tccacaggat
    19561 acttgtaaag cgattggcat tatcagcaac aacccggagt ttgccgatgt gcgtagcctg
    19621 gaagggcttt ccccgaccaa taaacccagt gtaccgattc tggcaattcc taccacagca
    19681 ggtactgcgg cagaagtgac cattaactac gtgatcactg acgaagagaa acggcgcaag
    19741 tttgtttgcg ttgatccgca tgatatcccg caggtggcgt ttattgacgc tgacatgatg
    19801 gatggtatgc ctccagcgct gaaagctgcg acgggtgtcg atgcgctcac tcatgctatt
    19861 gaggggtata ttacccgtgg cgcgtgggcg ctaaccgatg cactgcacat taaagcgatt
    19921 gaaatcattg ctggggcgct gcgaggatcg gttgctggtg ataaggatgc cggagaagaa
    19981 atggcgctcg ggcagtatgt tgcgggtatg ggcttctcga atgttgggtt agggttggtg
    20041 catggtatgg cgcatccact gggcgcgttt tacaacactc cacacggtgt tgcgaacgcc
    20101 atcctgttac cgcatgtcat gcgctataac gctgacttta ccggtgagaa gtaccgcgat
    20161 atcgcgcgcg ttatgggcgt gaaagtggaa ggtatgagcc tggaagaggc gcgtaatgcc
    20221 gctgttgaag cggtgtttgc tctcaaccgt gatgtcggta ttccgccaca tttgcgtgat
    20281 gttggtgtac gcaaggaaga cattccggca ctggcgcagg cggcactgga tgatgtttgt
    20341 accggtggca acccgcgtga agcaacgctt gaggatattg tagagcttta ccataccgcc
    20401 tggtaaatgc gctgatgtga taatgccgga tacgacgttt gcggcgtatc cggctcgccg
    20461 ttaccgtacc aaccgcaatt gctgtaaatt gccgtcgata tgcaaatcgg tttgtaagcg
    20521 ggcaatatcg cggcacagaa acgccatctc tttatgggtt tctaattttt tgcgccactt
    20581 ttcggcaacc gcatccagat tctcatatat cccttccaga ctctgaaact cgaccagcag
    20641 ctgcgtggcg ctttttggtc cgattcccgc aacacccggt acctttgaac tgctgatccc
    20701 cgcaagtccc cagtaatcgg gcaactgctg cggttgaacg ccaaattctt tatcgataaa
    20761 tggcgcatcc agccaacgtt tctggaagta atcacgaata cgtaatgtcg gtgaaagtaa
    20821 ctgacagtag cctttatctg tcgaaacaat cgttgcctga tgcccggcct gtgtcacttt
    20881 gaccgccagc gtggcggcta agtcatcggc ttcgttgccg ctggttgacc agcacgggac
    20941 gccgcgttgc tcaaaggcgg cgcgtaatgc aggcatctcg tcgtgcaact cttccggcat
    21001 tggcggtcga cccgctttgt aatctggtaa acgctgatga cgccagccgc tactgcggtt
    21061 ttcatcatca aaaacggcga ccgcgtgggt tggctggctg tgcataatga gctgatcgag
    21121 cgcatgctgg caggtttcga cacagggcga tccctgaacg gcatgaatgc gacgaataag
    21181 attcagtgca tcgacaataa gcaaatgaac agccacgata acccccttaa agatcaggcg
    21241 ataagggtaa cattcgatgc cggaacaggc tacggctgga tgagaaatcg ggaagaggcc
    21301 tcgcaaaacg aggcctctgg agagcgatta atcgcaggca acgatcttca ttgccaggcc
    21361 gccgcgagag gtttcgcggt acttggcgtt catatcttta cctgtttcgt acatggtttc
    21421 gataacttta tcgaggcaga cgcgcggctc gctggtacgg cgtagcgcca tacgtgcggc
    21481 gttcaccgct tttactgccg caatggcgtt acgctcgatg catggcacct ggacctgtcc
    21541 ggcgaccggg tcacacgtca gaccgaggtt gtgctccatg gcgatttccg ccgcgatgca
    21601 cacctgcgcc gggcttgcac ctaacagttc tgccagacca gccgccgcca ttgagcacgc
    21661 cacaccaact tcaccctggc agccgacttc agcaccagaa attgacgcgt tcatcttata
    21721 aagagaacca atggcactgg ctaccagcag gtaacgagcc agtgagttag cgttcacttc
    21781 gcggataaac ttatcgtagt acgccagcac agccgggata atcccgcacg caccgttagt
    21841 cggcgcagtc accacgcgac cgccagcggc gttctcttcg ttcactgcca gtgcaaacat
    21901 gttgatccag tcaacaaccg ccatcgggtc agtggtggtt ttatcctggc tgaccagcat
    21961 ccggcgtagt gccgcagcac ggcgtggtac gcgcagtttg ccaggcaaca cgccttcggt
    22021 agaaataccg cgctcaatac cgccgcgcat gacttcccag acgttcgcca ggtgctgttc
    22081 cagctcttct ttgctgtgca gcgccagctc gtttttcatc atcaggccag agagtgacag
    22141 cccggtttcc tgacaatgtt tttgcagatc ggctgctgaa ctgtacggat aaggaacttc
    22201 aaccggtgcg ctattctgct ggccaaaatg ctcttcatca acgataaagc caccgccaat
    22261 agagtagtaa gtctggctgt aaacgacttt atcgcccgcc agcgcggtaa tgcgcatacc
    22321 gttttcatgc agagaaaggt tgtctgcatg gaagttcatg cactgatcaa ccgggaactc
    22381 cacttcatgc tgaccgtttg ccagcatcag gcgaccatga gtattcacat cctgaataaa
    22441 accagggatg gaatcgatat ccacggtatc cggcaggtta cccgccaggc ccataataat
    22501 ggcgatatca gtgtggtggc ctttaccggt cagagagagc gagccgtaca cgtcaaccac
    22561 cacgcgggtc acgtctttaa gcagattacg ggcaatcaga tcgtcggtaa attgtttacc
    22621 cgctttcatt ggtccaacgg tatgagaact ggaagggcca atgccgattt tgaaaatatc
    22681 gaatacgcta atcataggaa atacatcgcg ttaaaacgga ggaagcgccg cccgaaagcg
    22741 gcgcgaaagg acttagctga acagagagta gaagattgcg gagattgcaa tcagacccat
    22801 cacgacaacg aatacgttgc tgatgtgacc gctgtactta cgcattgccg gtactttctg
    22861 aattgcgtac atcggcatca ggaacaggat catcgcgatg attgggccgc ccagggtttc
    22921 aatcataccc aggatgctcg ggttcagggt ggcaacaatc caggtcgtta ccagcatgaa
    22981 cagcgcagtg atacggttca gcttgttgat ttcgatagac ttacctttac cacgcagaga
    23041 tttaatcacc ataccgttga agccttcacg tgcgcccagg tagtggccga ggaaggattt
    23101 ggtgatagcg ataatcgcga taatcggagc catccacgcg ataaccggtg cgttaaagtg
    23161 gttagccagg taagacagaa tcgagatgtt ctgctcttta gccgcagcca ggtctgccgg
    23221 agtcaggctc agtacgcagc tgaagacgaa gaacattacg gtcagcacca tcatgatgtg
    23281 tgcgaatgcc aggatcttcg agcatttctg ttctgccata tcgccgtact cttcacgctt
    23341 cgcaacggcg aaagaagaga tgatcggaga gtggttgaac gagaacacca ttaccggaat
    23401 tgccagccac agggtcatcc acagaccgtt tccggttgca gatgcagtgt ccagagacag
    23461 cgtttccagt gctgcgccgt tccactgcgg gatcaggtac agagccagca gcatcagtac
    23521 gccaacaaac gggaatacca gaatactcat cgctttaacg atcatctgct caccaaagcg
    23581 aacgatggtc atcataccca cgatcaggat cagcgacaga atcgcacgcg gcggtggcgt
    23641 catacccagc tggtgagaca tgaagctttc aacggtattg gtgattgcca cgctataaac
    23701 cagcaggatc gggtagatag cgaagaagta gagcagggta atcagtttac ctgcgccaat
    23761 accaaagtgt tcttctacaa cctcggtgat gtcttcgccc gggtttttac cagacagtac
    23821 gaagcgagtc aggccgcggt gagcaaaaaa cgtcatcggg aacgcaagga tagccatgat
    23881 gatcagcggg atcataccgc caacaccggc gttgattggc aggaacagca cgcccgcgcc
    23941 gattgccgtg ccgtaaaggc ccagcatcca catggtgtct gtcttgcgcc aggcactacg
    24001 agagtctttc gacgcaatcg tgctggtttg agtcgtttcc atctatttct cctggaggaa
    24061 tgcaaataat tcagctttta gccagttcag cccgggaagg tgcctgaccg taaaaatgaa
    24121 atctgtctat cggcgatggc ttaatcattt accgccgtaa taatttgagg gcggaaagat
    24181 acacacaagt tatgtatcta tcagtgatct tgatctcaaa tacaataatc tacttggaag
    24241 atagattatc ccaacgcttt actctgtaaa tcaaaagtaa aagagaattt aagggcaata
    24301 tttgcccatt aaacgacatc tgacttcttc cggggcgcgc aatatatgta ggttgaactg
    24361 aaaaaatcaa tacttttagt taatcatggc gataatttct ttttcatagc gataaaatac
    24421 ggtttatgaa aatagggaaa cgtttgcgtt gcaacataaa ttgtatgaca tattaactgt
    24481 gatgatattt gtcgcgtgag ggtggtaaga aacattcata taacaaatga aatgagttga
    24541 tgagtaagtg accaaaaacg agaaaacccg gcaacaaacg tcaccggggg agcggagatt
    24601 acgtgcagat ttcgtagcaa gggatgtagg ctgagcctgg caacttcata cgatgctggg
    24661 caacaaaacc ctgtagcagg tcgtccatac gacgcataat ctctttatcg ccgttgattt
    24721 tgtaaggacc aaactcttca atggcgcgaa taccgacttc ttttacgtta cccgccacaa
    24781 taccggagaa cgcacggcgc aggtcggcag ccagcacttc aacaggttga tccgggtaaa
    24841 gcttcagatt agccatattc tcgtgagacg gctcaaacgg catttgcaaa tctggtgcaa
    24901 tgcgcattga ccagttaaag ctgtaagcgt caccagtgtc gcggcgattt tctttcacca
    24961 gcggcatcga ttttttcatc tgacgagcga cttcagcggc gtcatcaatg atgatgcggt
    25021 aatggcggcg cgcgttttcg cccagcgtat gtacgacaaa ctcgtccagt acgcggaagt
    25081 agtcggcgct ctctttcggg ccggtgagga tcaatggtaa aacctgatct ttgttggccg
    25141 ggttcattaa aattcccagc aaatacagca actcttctgc cgtacccaca ccgccaggga
    25201 agataatgat gccgtgagcg atacggacaa acgcttccag acgtttttcg atatccggca
    25261 tgatgatcaa ttcgttgacc agcgggttag gcggttcagc ggcgataatc gacggctctg
    25321 tcataccaat aaaacgactg tctttgtaac gctgctgcgc gtgtccgacc gcagcacctt
    25381 tcatcggcgc ttccatcgct cccggaccac agccggtgca gatattcagc tcacgcaggc
    25441 ccagctggtt tccgacgcga cgggcataca aatactcgtt ttcgttaatt gagtgaccgc
    25501 cccagcagac caccatattt ggcgcttcac ccacatgcag cgcgcgagcg ttacgcaaga
    25561 tggaaaagac caggttagtg atgtggacgg agttgtcgag attcaggttg ggaaaacgaa
    25621 cggtattatg gatttgcccg taaacgaaga gaatgtctcg cagaaccgcg aacaagttgg
    25681 cctgcaaagc gcgaataatt cgcccatcga caaaagcctc ttccggggga ttaatcagtt
    25741 ccagctttac gccgcgttca cggcgcagga cgttaatatc gaaattttca aaacgagaca
    25801 gcaattcttt gctgttatcg gtcaaactac cggagttcag tacggcaagt gaacagttgc
    25861 gaaacagttg atagaggtcg ctgctggcgg tgcgtttaag catatccact tccagctgcg
    25921 acaacatatc catggagcca agcgggctaa tatgtgtaat caagaaaaac tccttatggg
    25981 acgaatatcg tcattccctt tgattacaat agccctgcct gatgaccttt cacaaccttc
    26041 tgcgtgaatc cagcacgcaa aattgagaaa aaatttattg ccgaaccagt cttgtggtcg
    26101 atgggacaaa atcgctatta ctgcgccacg ggttaatgtc cagaccgcca cgacgggtat
    26161 aacgtgcgta aacgcttaat ttctctggct ggcagaagcg taacaggtca ttaaagatgc
    26221 gttccacgca ctgttcgtgg aactcgttgt gatgacggaa tgagaccagg taacgcagca
    26281 gtttttctct gtcaatttgg cgtccacgat actgaatttg gatcgaaccc caatctggtt
    26341 gatgggtgat caggcagttt gatttcagca ggtggctgac aagcgtctct tccactactt
    26401 tttcaccgct ggtggcattc tccagatagt cagtagtgaa ttcatagtta tcgatagtga
    26461 tatcctggtc atcaatgcag gtgccattga aatgacctat cggctggcct tccagttcat
    26521 caagacgata taacgccacg ctaaccttac cctgggcgca agtgcttaag tcgcgctcca
    26581 gcgtctggcg cacctcatcc cagttattaa aacgcgtctg gttaaaactg ttgagataga
    26641 gcttaaaact cttcgactca atcagattta cgctggtgta atcaagttca acatgaccga
    26701 cagcgacctg cggcaaacct ttcgcattca gccaggaaag ttcatacagc gtccagatat
    26761 ccgtaccgtg aaaaggcagg ttatccgctt tcagacccag cgggtcgcga ttcaggctgc
    26821 gtggaacgcc ttgcagtagg ctggcgtcat aggtatcccg gtaatcggtt gattttccaa
    26881 gagtcaggcc cgcaagtgcc tgatggtttg cataagaaga catgtttcac cgtcataaac
    26941 cagataaact aaacgacaag tgtatctggt ttaagtgaag agagaaatta gtggacgatt
    27001 tgaccgcaca agccctgaaa gattttactg cacgctactg cgatgcgtgg catgaagagc
    27061 ataaaagctg gccgttaagt gaggaactgt acggtgttcc ttcaccgtgc attatttcaa
    27121 ctaccgaaga tgccgtatac tggcaacccc agccgtttac cggggaacaa aatgtaaacg
    27181 cggttgaacg cgcttttgat attgtgatac aacccacaat tcataccttt tatacgactc
    27241 agtttgccgg ggatatgcac gcgcagtttg gcgatatcaa actgacattg ctgcaaacct
    27301 ggagtgaaga cgacttccgt cgggtgcagg aaaatctgat tggtcatctg gtgactcaga
    27361 aaaggctaaa actgccgccc acgctattta tcgcgacgct ggaagaagag ttagaggtca
    27421 tctcggtgtg taatctctcc ggtgaagtgt gcaaagagac gctgggaaca cgtaaacgga
    27481 cgcatttagc ctcaaatctt gcggaattcc ttaatcaact taagcctctt ctgtaatcca
    27541 aataccccat ctggttgtga gagatctctt acagactctg taggagatcg ccaggaaata
    27601 agcgaatact taaaaagata agaatcgtta ttttcattta aaatcaaaat gttgattgtt
    27661 aattcttaac tttcatatga aattttcctt aaggcattgt ctgtaagcgc cttgtaagac
    27721 ttcgcgaaaa agacgattct atcttcgtcg acagggagtc agacaacgaa gtgaacatca
    27781 ggatgatgac acttctgcag gacacaccag gatggtgttt cagggaaagg cttctggatg
    27841 aagcgaagag gatgacgcag gacgcgttaa aggacacctc caggatggag aatgagaacc
    27901 ggtcaggatg attcggtggg tcaggaaggc cagggacact tcaggatgaa gtatcacatc
    27961 ggggtggtgt gagcaggaag caatagttca ggatgaacga ttggccgcaa ggccagagga
    28021 aaagttgtca aggatgagca gggagcaaca aaagtagctg gaatgctgcg aaacgaaccg
    28081 ggagcgctgt gaatacagtg ctcccttttt ttattcctgc tatccttcgc ggcagttttt
    28141 cttattgagg ttgctttatg accactcatg accgcgttcg tctccagttg caggcgcttg
    28201 aagcgttact gcgtgaacat cagcactggc gaaacgatga accccagccg catcaattta
    28261 atagtaccca accgttcttt atggacacca tggaaccact ggagtggttg cagtgggtgc
    28321 tgatcccgcg aatgcacgat ttgttagata acaaacagcc gttaccgggc gcttttgccg
    28381 ttgcacccta ttatgaaatg gcgctggcga cggaccatcc gcaacgcgcg cttattctgg
    28441 ctgagctgga aaaactggat gctctttttg cggatgatgc gagctaatgc tggaaatact
    28501 ctatcaggat gaatggctgg ttgcggtaaa taaaccctcc ggctggctgg ttcaccgcag
    28561 ctggctggat cgcgacgaga aagtagtggt catgcaaacc gtgcgtgatc agattggcca
    28621 gcacgttttt accgctcatc gtctggaccg acccacttct ggtgtgttgt tgatgggatt
    28681 atccagcgag gccggacggc tgctggcaca acagtttgaa cagcaccaaa tccagaaacg
    28741 ttaccatgcg attgtgcgcg gctggttgat ggaagaagca gttctggatt atccactggt
    28801 ggaagaactg gacaaaatcg ctgataaatt tgcccgcgaa gataaaggct cgcagccagc
    28861 agtgacgcat tatcgcggtc tggcgaccgt agaaatgccg gtagcgaccg gacgttaccc
    28921 gaccacgcgc tacggcctgg tggaactgga gccgaaaacc ggacgcaaac atcagcttcg
    28981 ccgacatctt gcccatttgc gtcatccgat tattggcgat agcaaacatg gcgatttacg
    29041 ccagaatcgc agcggtgctg agcattttgg cctccagcgg ttaatgctac atgccagtca
    29101 gttgtcattg acgcatcctt ttactggcga gccgctgact attcacgcgg gcctggacga
    29161 cacctggatg caggcgttat cacaatttgg ctggcgcggt ctgcttcctg aaaatgaaag
    29221 ggttgagttc agtgcgccat cgggtcagga tggagagata agttcctaat ccagggagaa
    29281 aacggtatgg cggaaattgg tatttttgtc ggcaccatgt acggaaattc gctgttagtg
    29341 gcggaagaag cagaagcaat tctgaccgca cagggccaca aagcaacggt atttgaagat
    29401 cctgaattaa gcgactggct gccctatcag gataagtatg ttctggtggt tacgtccacg
    29461 accgggcagg gcgaccttcc tgatagcatt gtgccgctct ttcagggaat caaagatagt
    29521 ctgggcttcc agccgaatct gcgttatggc gtgattgcgc tcggcgacag cagttatgta
    29581 aatttctgca atggcggcaa acagttcgat gccttgttgc aggaacagag cgctcagcgg
    29641 gttggcgaaa tgctgttgat tgacgccagt gagaacccgg aaccggaaac ggaatcaaat
    29701 ccgtgggtcg aacagtgggg cacgctgttg tcctgatatg ttcagcgagc ggtaaatgtc
    29761 gttttagcgg tgctgaatcg aatattttca ggcaaatgcc agtaaaaact gcttcatagc
    29821 gcggattttt actggcgttt gcctggagtc aagcgatcca tttcatactc ttctttattt
    29881 cttcgtttta acccttcctt tcttgttctt gttttcattt ccgtgaagtg gattccaccg
    29941 tccagggcta atgccaaaat cgggcctcat tgaacgcatt aatgttgtgt tgttgcacgg
    30001 tgagccgcta tggcgcgctt tttatactgc tattgccaga tataaacacg cgccgtattc
    30061 ggcgaacgac ctataaaaac ggcaaaaaac accctacgtc acctctgatt tcctggctat
    30121 gtcgcagtcc agagtgagcg tggctaacgc gaattttcag gagtgcaaca atgagttctt
    30181 taagtcaggc tgcgagcagt gtggaaaaac gcacgaatgc tcgttactgg atagtggtga
    30241 tgttgtttat cgtcacatcc ttcaactacg gcgaccgcgc tacgctttct attgccggtt
    30301 cggaaatggc caaagatatc ggccttgatc ccgtgggaat gggctatgtg ttctctgcct
    30361 tctcatgggc ttatgttatc gggcagatcc ctggtggctg gttgctggac cgttttggtt
    30421 caaaacgcgt ctacttctgg tcgatcttta tctggtcgat gtttaccttg ctgcaaggct
    30481 tcgtcgatat ctttagtgga ttcggcatta tcgttgccct gtttactctg cgcttcctgg
    30541 tcgggcttgc tgaagcgcca tctttccccg gcaacagtcg cattgttgcg gcctggtttc
    30601 cggcgcagga aaggggaacg gcggtgtcga tttttaactc cgctcaatac ttcgcaacgg
    30661 tgatcttcgc gccgattatg ggctggctga cgcatgaagt gggctggtca cacgtcttct
    30721 tctttatggg cggtctgggg attgtcatca gctttatctg gttgaaagtc atccacgagc
    30781 caaatcaaca tccgggggta aatcagaaag agctggagta tatcgccgcg ggtggcgcgc
    30841 tgatcaatat ggatcagcaa aacaccaaag ttaaagtgcc gttcagcgtg aagtgggggc
    30901 agatcaaaca actgctcggg tcacggatga tgatcggcgt ttatatcggt cagtactgta
    30961 tcaacgccct gacttacttt ttcattacct ggtttccggt ttatctggtg caggcgcgtg
    31021 ggatgtcgat tctgaaagcg ggctttgtgg cttccgttcc ggcggtttgc ggttttatcg
    31081 gcggtgtgct gggtgggatt atttccgact ggctgatgcg ccgcacggga tcgctgaaca
    31141 ttgcgcgtaa aacaccgatc gtaatgggca tgttgctgtc gatggtgatg gtgttctgca
    31201 actacgtcaa cgttgagtgg atgatcatcg gctttatggc gctggccttc ttcggtaagg
    31261 gcatcggggc gctgggttgg gcagtaatgg cagataccgc gccaaaagag atcagcggtc
    31321 tttccggtgg cctgttcaac atgttcggta acatttctgg catcgtcacg ccaatcgcaa
    31381 ttggttatat cgttggcacg actggctcct ttaatggggc gctgatttat gttggtgttc
    31441 atgccttaat cgcggtactg agctacctgg tgctggtggg cgatatcaag cgtatcgagc
    31501 tgaaacctgt cgcggggcaa taagatgacg acacaaccca gtcctgttat tactgatatg
    31561 aaagtc
//
LOCUS       DAEVZA010000044        31490 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEVZA010000044 DAEVZA010000000
VERSION     DAEVZA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.044"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            68..183
                     /gene="rrf"
                     /locus_tag="LAF92_002108"
     rRNA            68..183
                     /gene="rrf"
                     /locus_tag="LAF92_002108"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            198..273
                     /locus_tag="LAF92_002109"
     tRNA            198..273
                     /locus_tag="LAF92_002109"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:231..233,aa:Thr,seq:ggt)
     gene            313..428
                     /gene="rrf"
                     /locus_tag="LAF92_002110"
     rRNA            313..428
                     /gene="rrf"
                     /locus_tag="LAF92_002110"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(660..1418)
                     /locus_tag="LAF92_002111"
     CDS             complement(660..1418)
                     /locus_tag="LAF92_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBI9791115.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1426..2529)
                     /locus_tag="LAF92_002112"
     CDS             complement(1426..2529)
                     /locus_tag="LAF92_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBI9791116.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2539..3720)
                     /locus_tag="LAF92_002113"
     CDS             complement(2539..3720)
                     /locus_tag="LAF92_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBI9791117.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3788..4813)
                     /locus_tag="LAF92_002114"
     CDS             complement(3788..4813)
                     /locus_tag="LAF92_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI9791118.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5244..5465)
                     /locus_tag="LAF92_002115"
     CDS             complement(5244..5465)
                     /locus_tag="LAF92_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791119.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5718..8822)
                     /gene="acrF"
                     /locus_tag="LAF92_002116"
     CDS             complement(5718..8822)
                     /gene="acrF"
                     /locus_tag="LAF92_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HBI9791120.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8834..9991)
                     /gene="acrE"
                     /locus_tag="LAF92_002117"
     CDS             complement(8834..9991)
                     /gene="acrE"
                     /locus_tag="LAF92_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBI9791121.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10390..11052
                     /gene="acrS"
                     /locus_tag="LAF92_002118"
     CDS             10390..11052
                     /gene="acrS"
                     /locus_tag="LAF92_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBI9791122.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(11055..11234)
                     /locus_tag="LAF92_002119"
     CDS             complement(11055..11234)
                     /locus_tag="LAF92_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBI9791123.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11318..12202)
                     /gene="yhdJ"
                     /locus_tag="LAF92_002120"
     CDS             complement(11318..12202)
                     /gene="yhdJ"
                     /locus_tag="LAF92_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBI9791124.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(12288..12584)
                     /gene="fis"
                     /locus_tag="LAF92_002121"
     CDS             complement(12288..12584)
                     /gene="fis"
                     /locus_tag="LAF92_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBI9791125.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12610..13575)
                     /gene="dusB"
                     /locus_tag="LAF92_002122"
     CDS             complement(12610..13575)
                     /gene="dusB"
                     /locus_tag="LAF92_002122"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBI9791126.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13904..14785)
                     /gene="prmA"
                     /locus_tag="LAF92_002123"
     CDS             complement(13904..14785)
                     /gene="prmA"
                     /locus_tag="LAF92_002123"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBI9791127.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14797..16248)
                     /gene="panF"
                     /locus_tag="LAF92_002124"
     CDS             complement(14797..16248)
                     /gene="panF"
                     /locus_tag="LAF92_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBI9791128.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGHAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(16238..16480)
                     /locus_tag="LAF92_002125"
     CDS             complement(16238..16480)
                     /locus_tag="LAF92_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBI9791129.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16589..17938)
                     /gene="accC"
                     /locus_tag="LAF92_002126"
     CDS             complement(16589..17938)
                     /gene="accC"
                     /locus_tag="LAF92_002126"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBI9791130.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17949..18419)
                     /gene="accB"
                     /locus_tag="LAF92_002127"
                     /gene_synonym="fabE"
     CDS             complement(17949..18419)
                     /gene="accB"
                     /locus_tag="LAF92_002127"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBI9791131.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(19397..20371)
                     /locus_tag="LAF92_002128"
     CDS             complement(19397..20371)
                     /locus_tag="LAF92_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI9791132.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            20523..22463
                     /gene="csrD"
                     /locus_tag="LAF92_002129"
     CDS             20523..22463
                     /gene="csrD"
                     /locus_tag="LAF92_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBI9791133.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            22768..23811
                     /gene="mreB"
                     /locus_tag="LAF92_002130"
     CDS             22768..23811
                     /gene="mreB"
                     /locus_tag="LAF92_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBI9791134.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            23877..24980
                     /gene="mreC"
                     /locus_tag="LAF92_002131"
     CDS             23877..24980
                     /gene="mreC"
                     /locus_tag="LAF92_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBI9791135.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            24980..25468
                     /gene="mreD"
                     /locus_tag="LAF92_002132"
     CDS             24980..25468
                     /gene="mreD"
                     /locus_tag="LAF92_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBI9791136.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            25477..26070
                     /gene="yhdE"
                     /locus_tag="LAF92_002133"
     CDS             25477..26070
                     /gene="yhdE"
                     /locus_tag="LAF92_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBI9791137.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            26060..27529
                     /gene="rng"
                     /locus_tag="LAF92_002134"
     CDS             26060..27529
                     /gene="rng"
                     /locus_tag="LAF92_002134"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBI9791138.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            27597..31397
                     /gene="yhdP"
                     /locus_tag="LAF92_002135"
     CDS             27597..31397
                     /gene="yhdP"
                     /locus_tag="LAF92_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBI9791139.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINSDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNIELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
BASE COUNT         7892 a         7969 c         8070 g         7559 t
ORIGIN      
        1 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
       61 gaatttgcct ggcggcctta gcgcggtggt cccacctgac cccatgccga actcagaagt
      121 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
      181 ggcatcaaat ttagcgtgct gatatggctc agttggtaga gcgcaccctt ggtaagggtg
      241 aggtccccag ttcgactctg ggtatcagca ccacttttta ggttaaagtt cggcagatta
      301 gaaaagaatt tgtctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
      361 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
      421 tgccagacat caaataaaac ataaggctca gtcggaagac tgggccttat gttttatttg
      481 atgtttgtcg gtgaacactc tcccaaccag gacaaatccg ccgggaacgg atttgaacgt
      541 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccagacatca
      601 aatcaagcaa aaaggccatc cgaaaggatg gcctttttgc ttttcgaact aacattcaat
      661 taatggatta cctgcgataa aaatgccctc gtacgctctg atttaggatg cgtaaaaaat
      721 tcatcaggtg cagcttgctc cactatttcc ccacgatcca taaaaatcac ccggtcagcg
      781 acggttcgcg caaaccccat ctcatgcgtt acacacaaca ttgtcatacc cgattgcgcc
      841 agcccaatca tcgtatccag cacctctttc accatctccg gatcgagcgc cgacgttggc
      901 tcatcaaaca acataatttt cggcttcata cacagcgaac gcgcaatggc aacgcgttgc
      961 tgctgaccac cagaaatttg tccgggaaac ttatgcgcat gttcggcaat tctcacccgc
     1021 tcaaggtaat gcatcgccag agcttcagcc tctttctttg gcatcttgcg tacccaaatc
     1081 ggtgccaggg tacagttctg tagaacggtc agatgaggga agagattgaa atgctgaaag
     1141 accattccca cttcctgccg gacgcgctca atattgcgga tatcttcatt aagttcgatg
     1201 ccatctacca cgatccgtcc ctgttgatgt tcttccagat gattaataca acgaatggtt
     1261 gtcgatttac cggaacctga agggccacac agaacgatcc gttctcccgg ttgcacggtt
     1321 aaatttatat ttttcaaaac atggaattgt ccataccatt tattgacgtt ttccagcgta
     1381 atcatcgcgt tagcaggttg cagtaaaatt tggctcatag tgtcctcaat gcggtgtacg
     1441 cccggtgtta aaacgttttt ccagatgctg gctatagcgc gacatgctga aacagaagat
     1501 ccagtagatc agcgcggcga aaacataccc ttccgtcgac atacccaacc aggtgggatc
     1561 aacggttgcc tgctgaacgc tactgaaaag atcgaacaac ccgataatga tcaccaggct
     1621 ggtatctttg aagagtgcga tgatggtatt taccagccca ggaattacca gcttcaacgc
     1681 ctgtggcaga ataaccagcc cctgagtttt ccagtaaccc aacgccagcg actctgccgc
     1741 ttcatactgc cctttaggca gtgcctgtaa tccgcctcgc acgacttccg caacatatgc
     1801 tgactgaaac aggatcacgc caaccagcgc gcggatcagt ttgtcgatac tggtgccttc
     1861 tgccataaac aacggcagca tgaccgaaga cataaacaat acggtaatca gcggtacgcc
     1921 gcgccagaac tcgataaaaa tgaccgataa gatacgcaca atcggcatat gggagcggcg
     1981 acctaacgcc agtaagatcc cccacggtag cgcccccgca atcccaacgg atgcaataat
     2041 taaagttagc gtcagcccgc cccattgccg ggtttcaacc cgctccagac caaaaaaacc
     2101 gccatacatc agccaccaga caatcagtgg gtaaatcacc gcccagaccg caatatagcg
     2161 accgcgatgc gggagtattt tccagaacat tggtgcgatt gacacaaggc caatcagtaa
     2221 tgccaggtta attcgccagc gttggtcgtg tgggtaaagt ccatacataa actgaccaaa
     2281 tcgttcgtgg atgaagaccc aacaggcacc ggcttttgtg cagtcggcac gcgttgagcc
     2341 aacccaatta gcctgcaaaa atgcccagtt cagcaacggt gggatcagtt cccacatcaa
     2401 ccatatgcag ccaatagtca gcaggctatt gctccagctg gagaacagat tttttcgcac
     2461 ccacaccatc gcgcggcttg agttatggct cgctgggcgc gagggctgag acagcaatac
     2521 ttttgtcatc atgactcctt agcgttcaac gatcgcgatg cggcggttat agatattcat
     2581 cagcagcgag atagtcaggc tgataatcag atagaccgac atggtcatgg ctatcgtctc
     2641 gatggcttgc cccgtctggt tcagcacggt gccagcaaac agcgaaacca tatcgggata
     2701 gccaatagcg gcggcaagag aggagttttt gacgatgttg agatactggc tggttaacgg
     2761 cggaataatc actcgcagtg cctggggaat aatgacctgg cgtagcgtaa ccgggttggg
     2821 taatcccagt gaccgagccg cttcatgttg accataaggc actgcctgga tcccagcgcg
     2881 gataatctcg gcgataaatg cagatgtata aaccgaaagt gccagcgtta aggctgccag
     2941 ttcaggaatt aaaaccatcc cgccgcggaa gttaaagcct cgtagggctg ggacatccca
     3001 gtgtagtgct gcgccaaaaa gccattgcgc cagtaaaggc aaaccaatga tcaacactgc
     3061 ggcgatcggc caggtgcggc gcagttgtcc ggtctttatc tggtatgttt tattaaagcg
     3121 gaatagccca acagaaagga ctatagccat aacaacagcc aggataaacg caataaatcc
     3181 gtctcccagc tgcggtgacg gaatataaag cccacgattg ctcaaaaagg ccagatcaaa
     3241 cgcgctcaca gcctggcgtg gtccgggcaa attgcgcaac acggcaaagt accagaagaa
     3301 gatttgcagc agcgggggaa tattacggaa gatctcaata taaattgtgg aaagctttcg
     3361 tagcagccag ttatccgaaa gtctcgccag accgataaag aagcccaaaa cagaagcgaa
     3421 cacaatacac aatgcagaaa ccagtagcgt attgagtaag ccgacaataa aaacgcgtcc
     3481 gtaggtgtcg ccctgctggt aatcgatcaa atgctggaca ataccgaagc cagcgccgcg
     3541 atccagaaag gcaaaacctg aagtaatgcc acgattactg agattcgtta cagtgttgtg
     3601 aaataaccaa ccaacaatgc cgacaacagc aacaacggca aggatctgga ataaccaggc
     3661 acgaaccgta gggttggcaa aggagagtga gcctttaacg gttgagcggc gatgagacat
     3721 agaaacctcg gaaagcataa cgctgaagag agcgccgccg tagcgacgct cgttacctca
     3781 caccgactta acgcacgggc ggcgcgtact gaataccgcc gttattccag agattatttt
     3841 gcccacgttt aattttcagc gggctttctg aacctacatt acgctcgaaa atttccgagt
     3901 agttacccac ctgtttgatg atgttatagg cccatttatt atccaacttc agatccttgc
     3961 cgtaatcgcc ttctttaccc agcagatgtg ccatatcagg cgttgctgga ttagccgctt
     4021 tttcatcgac gttctgggaa ttgatgccca tctcttcagc attcagcatg gcgaaaagcg
     4081 tccagcgtac aatcgagaac cattcatcat cgccacgacg aactaccggc ccaagcggtt
     4141 ctttagagat aacctccggt aagacaatcc attcagctgg gttgcttaat ttgatgcgca
     4201 gggcatacag ttgcgactga tccgaggcca gcgtatcgca gcgaccagat tccagtgcct
     4261 tcgctgattc gtcagagcga tcgaaagtca ccggtgtgta cttcatattg tttgccttga
     4321 aatagtcggc aacgttgagt tcggtatcag tacccgcctg aatacagacg gtagcgccat
     4381 ccagttcttt cgcgcttttt agccccgctt tatcgtgcgt caggaagcca atgccgtcgt
     4441 aataagtgac gccagtaaat gccattccca tcccggcgtc gcgagatgaa gtccaggtcg
     4501 tattacggga gagcaaatcc acttcccctg actgtaaagc cgtgaagcgt tcttttgctg
     4561 tgagtggggt atatttcact ttcgtgtcgt caccaaatac agcagcggca acaccacgac
     4621 aaacatcaac atcaatacct gaaaacttac cgtctgcatc ggcataagag aacccaggta
     4681 atccatcact gatcccgcat tgcacaaaac cttttttctg aacggcatca agcgtcgcgc
     4741 cagcatgcgc ctgatttgca acggcaagca gcacgctggc ggcagccagt gtggctatca
     4801 tcatcttttt cataatgctt cctgtgaggc gaaaattatc gttataggac gtttgggaaa
     4861 cgtcttttac ctgtctgtgg ccatcctctt ataagcaaaa gtagtgccag cttttgcgcc
     4921 cgatttcgcg tcgcaataag ctgagtgtag acaggaaata ataaaaacag cgccccgcaa
     4981 tggtgcgaaa atgaaacatg cgccacattt tggcgcaaaa tatttacgcg tgttttacta
     5041 agcagaataa tgattgttag cgtgagtata tttctggtgg gaagtgtgaa agttaaattg
     5101 atgcgttatt tcgaaagcag cgaaataacg cgatataaca tgctaaatca gcagagaatt
     5161 agccagcaga atatagcaat attttatctt atgtaaagat actgtatcgt taaacagagc
     5221 gcgaaactcc gcgccctgtt cagttatttc gacagagcga tgatccccat cgtcaggcca
     5281 gcaacagccg ctgccccgcc agcaatagcc ccagccgttt cccagttatc ctgagtcgtg
     5341 cctttcatac aggtattggt gtgaaccaga cgaccctgat cgtcataaac cggaacgcag
     5401 ggcgaatcgt gagcacagcc cgccagcaat gcggtgagca atgccacagg aattaatctt
     5461 ttcatggtgt tacctcagta ataaacgcta acgcttaata agttaactct cttattaacg
     5521 agctgaatgc cattttatca ccggaggtta taaatattgc ggaatgaata atgacaaatt
     5581 atgtgaattg taaaaatagt gaagggagcg ggaactatag aaagttttta aagagtgagt
     5641 ggaagaatat cttattaatt tttatgggtt tcactgaaaa taataaaggc acccgaaagt
     5701 gcctttatgt ttctgattta tcctttaaag caacggcgga tcaccacaaa gaacaccggt
     5761 acgaagaaga ttgccagcaa cgttgcagag accattccgc ccattacccc gatacccact
     5821 gcgttctgcg cgccactgcc ggcaccgtta ctgatagcta gcggtaatac gccgagaata
     5881 aaggcgagag aggtcatcag gataggacgc agacgcatac gtactgccat tagtgtcgct
     5941 tcaacaacac ctttaccctc tttctccatg agatctttag cgaactcaac aatcaaaata
     6001 gcgtttttgg ccgacaagcc aattgtcgtt agcaagccca ccataaagta gacgtcattt
     6061 ttttgattaa agagtgtcgc cgccagcagc acgccgacaa tccctaacgg cacgaccaac
     6121 ataaccgaga caggaattga ccagctttca tagagtgcag caaggcacag gaaaacaacc
     6181 acaaaggaaa ttgctaccag agcgggagcc tggtttcccg ataagcgttc ctgatacgac
     6241 atacccgtcc agtcataacc aatgcccgca ggtaattttg acgcaaggtt ttccatcaac
     6301 gccatggcat cgccggaact ggttcctggc gcggcttccc cctgaatctc cattgacggc
     6361 agaccgttgt agcgttccag tcgcggagag ccatacaccc aatgtgaagt ggtaaaggcc
     6421 gagaatggca ccatttcgcc gttggcgctg cggacataaa gtttatcgac atcttctggc
     6481 agcatacgga atttggcatc cgcctgaaca tacacctttt tcacgcgacc acggtcgatg
     6541 aagtcgttaa cgtaagtccc acccaacgcc gttgaaatgg tctgattgat gtcagaaagt
     6601 gaaacaccta atgcctgcgc cttttcctgg tcaacttcca gtttaaactg cgcggtgtct
     6661 tccaggccat taggacgcac gctgactaag ctggcaggat gttgcgccgc catgccaagc
     6721 aactggttac gggcctgggt cagggcatcg tgacccagcc cagcctgatc aattaactca
     6781 aagtcgaaac ccgttgccgt gcccagttca acaatggctg gcatattgaa tggaatgaca
     6841 aaaccgtcgc ggatcttgcc caattccatt ttggcacgat ggattaccgc ttccgcactg
     6901 ttttcatcac cattacgctc ttcccacggt ttcagactga cgaaggccat accggcgttt
     6961 tgtgcctggc cgctgaagct aaagccgtta accgtaaaga cactttcaac gttcgctttc
     7021 tcgttcttca gatagtaatc cgtcacttga tccaacactt tttgcgtccg ctcttgcgtc
     7081 gcgccagcgg gtaactgaat catggtcaga aagacaccct gatcctcttc aggtaagaag
     7141 gaagacggaa gacgtaaaaa caacaccacc attcctgcaa caatcagcgc atagatcagt
     7201 aaatatcgtc ctgtggaacc gaggattttg ccgacgctgt tggtgtagtg gttaacgcta
     7261 tgatcgaagg tagtattaaa ccagccgaag aaaccgccct tattttcgtg atgctcagca
     7321 gagacgggtt taagcagcgt tgcacataac gcaggggtaa gaatcaatgc caccagaaca
     7381 gaaagcgcca ttgccgaaac gatggtgata gagaactggc gataaattgc cccagtagaa
     7441 ccgccgaaga atgccatcgg aataaatacc gctgacagca ccatcgcgat acccaccagt
     7501 gcgccctgga tttgcgacat tgatttttcc gtcgcttctt ttggcgggag cttatcctcc
     7561 atcatcacgc gctcgacgtt ctccaccacc actatcgcat catcgacgag cagcccgatg
     7621 gcaagcacca tcccgaacat cgttagtgtg ttgatggagt aaccaaaagc ggcgaggatg
     7681 gcaaacgtcc ctaacaacac cacgggtacc gcaatggtgg ggatcagcgt tgctcgcata
     7741 ttctgcaaga acagatacat caccaggaac accagcataa tggcttcgaa cagcgttttt
     7801 accacttcgt gaatagaaag ctggacgaat ggcgtggtgt cataagggta gagaaccttc
     7861 attccctgcg ggaagaatgg ctgtaattcc gccagttttg ccttaatggc tttcgcggta
     7921 tcgagagcat tcgcgccggt tgccagctta atccccaggc ccgccgccgg ttttccgttg
     7981 atacgagcga taacgttata gttttcaccg ccaagttcaa cccgtgcgac atctttcagg
     8041 cgtaccaccg agccgtcact gtttacgcgc agggtcactt tgccgaattc ttccggattt
     8101 tttaaccgcg tttgagcaat aatcgaggca ttcaactgtt gccctggtaa cgctggcgtt
     8161 ccgcccaact gtccggcagc gatctgatcg ttctgtacct tcaactggtt aatcacatca
     8221 accggtgtca gtttatattt gtttagcaga tcggcgtcca gccagatacg catcgcatac
     8281 tgtgcgccga aaagctgtac gtcaccgacg ccattcagac ggctaagcgt atctttaacg
     8341 ttagaggcca catagtccga gatatcgtcc tgtgtggtgt ctgggttatc agagacaaag
     8401 cccgccacca tcaaatagct gctactggac ttttcaacac tgatcccctg ctgctgaacc
     8461 tcctgcggca gcaacggcgt ggcgagctgg agtttgttct gcacctgcac ttgcgcgata
     8521 tcaggatcgg tcccggactg gaaggtaagg gtaattgtca cgctaccggc ggaatcgctg
     8581 gtggaggaca tatacatcag gttatcgata ccattcatat tctgttcgat aacctgcgtc
     8641 accgtatcct gcacggtctg cgcatcagca cctggatagt ttgccgaaac agaaaccgca
     8701 ggcggtgcaa tggttggata ctgagcgacg ggcaattgta ggatcgccag tgcgcccgcc
     8761 atcatcagaa taatggccag cacccatgca aatatcggtc gtcgaataaa aaagtttgcc
     8821 atgtcagatt accttacttc gatgcagtat ctgcgggggt atcggtagtg gctttaacct
     8881 gctctcccgg acgcgctttt tgcaggccgc tgacaatgac ttgatcgcca gatttcagac
     8941 cttcgctaat caaccattta tcgccaatcg cctgactggc aacgacaggg cgcgcttcca
     9001 cctgactttt atcgttaaca atcagcacgg ttgcatcacc acgcggtgtg cggctaacgc
     9061 cttgttgcgg gataagaatg gcgtcaggtt ggacgccttc atcaatccgt gcgcgcacaa
     9121 acatacccgg caaaagcgta tgttgcgggt tagggaagac ggcacgtagg gttatggagc
     9181 cagtgctttc atcaacggtc acatcagaga attgcagcgt acctttcagg ggataggttt
     9241 gaccgttttc catgaccaac tctacgttgc tggtggcgtt ttccttatgc aaatttcctt
     9301 gctctacgga ttgcttcagc ctcataaaat cgttgctgga ttgggtcaca tcaacgtaga
     9361 taggatcgag ctgctggaca gtcgccagtt cagtcgtttg cccattagtg acaagagcgc
     9421 cttcggtcac ggtcgatttg ccgatacgtc cgctaattgg cgcagtcact ttggtataag
     9481 cgagattgat gcgagcgctt tcgactgtgg cttttgcggc aatcacggcg gcatcggcct
     9541 ggcgagcatc agcaatggcc tggtcgtact cctgctggct gatgtatttc gtacccacga
     9601 gcggaacgta acgttttacc gtcaaatgcg cgatggcggc ggcggcttca cttttcgcca
     9661 gttcgccttt cgcgctgtca taatttgcct gataggtcgc gggatcgatc tggtacaggg
     9721 actggcctgc ttgcacatcg ctgccttcag tgaaattgcg attcagtacg atcccgctaa
     9781 cctgtgggcg aacttcggct atacggtaag cattggtgcg gcctggtaat tcagtcttaa
     9841 cttctaacgg ggccgttttt acaatatgaa cggtaacctg cggttcaccg acgtgagctt
     9901 tctcttctcc cttatcgtta caaccggcga ttaaagccgc ggagatcaga ataaaggagg
     9961 gcaggaggaa aaacctggca tgtttcgtca ttactattcc tcaaaaaacc aaaagcgcgt
    10021 tatttaccca aaaaggcaac gcgttaactc gcagaaagaa aaatacagtt cgctatccta
    10081 caaattatca ttcgtcgatg taaggaatag ttatgaatac aggcgtctca aggcacataa
    10141 acacaaaaaa agattaatat tctactgttt tattttgacg cggattgaaa gaggcggaat
    10201 taaaacatcg taaattgaaa tatatattga tgtagtgaat gtatcttagg taaataatat
    10261 atattattta tttaacgatt tatagcaact caatattagc ctctcgtata aatacacatt
    10321 aggtgataga ttaaccttcg ctattttctc actctgtgtc gaatatattt atttcctgaa
    10381 taattaatca tggcaaaaag aaccaaagcc gaagctctga agacccggca agaactgatt
    10441 gaaactgcca tcgcccagtt tgcgcagcat ggcgtaagca agacgacact caacgacatt
    10501 gccgacgctg ctaacgttac gcgtggcgct atctactggc acttcgaaaa caagactcaa
    10561 ctgtttaatg agatgtggtt gcaacagcct tcattgcgag agttaatcca ggatcacttg
    10621 acggctgggt tagagcatga cccatttcaa caattgcgtg aaaaattgat tgtcggcttg
    10681 caatatattg ccaaaattcc ccgccagcag gcgttgctga aaatcttata tcacaaatgt
    10741 gaatttaatg atgagatgct ggccgaggga gtgatacgcg aaaagatggg cttcaatccg
    10801 cagactctcc gcgaagtatt gcaggcgtgt cagcaacaag gttgtgtagc aaataacctc
    10861 gatttagatg ttgtgatgat tattattaat ggtgccttca gcggaattgt tcaaaactgg
    10921 ttaatgaata tggcgggtta tgatctttat aaacaagccc ccgctctggt cgataacgta
    10981 ttaagaatgt tcatgccaga tgaaaacata acgaaattaa ttcatcaaac gaatgaatta
    11041 agtgtcatgt aaattcacat tctgtcctga aaattcagtt cgtcacagtt aaccagtttt
    11101 agtggattaa ctgagttcat attgcggcat ttgtctgttt ctgttgtcag cagttcaatc
    11161 cactgcatca gtaaagtatc aaacagaaaa acgaagacag cgaaaacgac gagccaccaa
    11221 tacttgcgaa tcattacctg agcctgtgca tgtttaacca agtctggcag gaaatataca
    11281 cgaaatgaat aatctgaaaa ccaggcttac acttccttta ctttgcaatg agatcggggt
    11341 caacttcgct taaccggctt cgttttgaca ggttgccggt ctttcttttt ttcactttcg
    11401 ccagttcttc cgcagagtaa tgcgacgcga catccagccg tcgaagcccc attttgatgt
    11461 actcgctgtt gatctcaata ccaatgaatt ttcgcccgct ggagacggct acggcaccgg
    11521 tagtaaagct accggcaaac gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa
    11581 taatgcgttt cagtaaggct tccggttttt gcgtcgggtg gttctcatat tcatccatta
    11641 aataacgcac gcgcggaaaa tcccagacgt tacccggtac tttttgatga ttgtatggct
    11701 gtggaggatt tttgcgataa tcgatcaacg cgcgctgcga tccggttttg gcttctacca
    11761 gaatagcatc accgttgaat gtgtagttct ttgcgtcttt caccatcatc aggatgggtt
    11821 cgtacatgga gccgtagtgt tttttcgcct gtactccaga actgtcatat gaccagacga
    11881 tgcgactttt gatggtaaaa agcttgcggc actggagatc gataaagggc atgttttccg
    11941 tactgttcat aatgtacatg ctgccctgct ttttcagaac gcggtggcac tctgcaatca
    12001 cttcaaacag ccagtcgata aacagatctt ctttccaggc ttcgatcaga ccatcaaaat
    12061 ttttaccgat gttatacggt gggtcggcaa agatcagatc gacgctttca gtaggtagct
    12121 ttttaagttc ggcaagggca tcaccgtgaa taatggtctt agcttcatta ccaaaccggg
    12181 tcggttcaca tcctgttctc atggtcactc cctttgtgac acctataaaa aaggcgcttc
    12241 cccatgccga gtagcgcctt tttaatcaag catttagcta acctgaatta gttcatgccg
    12301 tattttttca attttttacg cagcgtacca cggttgatgc ccatcatcag cgcagcacgg
    12361 gtctggttac cacgggtgta ttgcatcacc atgtccaaca ggggctgttc tacttcagcc
    12421 agtaccagct catagaggtc attcacatcc tgaccattca gttgagcaaa atagttcttc
    12481 agtgcctgtt taaccgagtc acgcaggggt ttttgggtta cctgatcctg agagttaacg
    12541 gtagaaacgg tcagtacgtc agaatttacg cgttgttcga acatagttct gtcagctctt
    12601 tatttctgtt tacgcaaaat tttcgaagta tgcctccaac gcctccagct gttcgctggc
    12661 atcctcaatg gcgttgaatg tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata
    12721 ccaggaaacg tgtttacgtg caattcggta cccttttgcc ggaccataaa agtcatgcag
    12781 ttcccgaacg tgcgcgcaaa gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa
    12841 ctccccagtg tccagataat gctggatttc ccgaaagatc cagggtcttc cctgagctgc
    12901 gcggcctatc atcagggcat ccgcccctgt atagtcgagc acagctctgg ctttaagcgg
    12961 gtcagtaatg tcgccattcg cgataaccgg aatggaaact ttctgcttaa ctgcccgaat
    13021 actgtcgtac tcagcttctc cattgaacaa acaggcgcgt gtacggccat gaatggtcag
    13081 agcctgaatg ccacagtctt cagccagttg ggcaatctct tcgcagttac ggtgttccgg
    13141 tgcccagccg gtgcgaatct tcagggtaac aggaacgtcc actgcattga cgacctcggt
    13201 aaggatcgat ttaacgacat ccgggtactg caagagggct gaacctgcga gcttgcgatt
    13261 cactttttta gccgggcaac ccatattgat atcaataatc tgggcaccgc tttccacgtt
    13321 aatacgtgct gcatctgcca tttctttcgg atcgctacca gcaatttgca cggtgcgaat
    13381 accgggttca tcaatgtgca ccatccgtaa acgagatttg tcgctttccc aaacctgtgg
    13441 gttagaagac atcatctcgg atactgtcaa tccggctccc atctcgtagc acaacgtccg
    13501 aaaaggtctg tctgtaatgc cagccatggg cgctgcgatc aggcgatttc tgagctgata
    13561 ttgtccgatg cgcatgagtt aagaaatgac catactgtga ctgcaaggcg gcgtatatta
    13621 cgcatttttt gcacgagatg aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag
    13681 aattgcattt aaaatgagcg tggtgcgata attactcata aaaatcatca tattagaaaa
    13741 tagtgactaa aatttacact caaagaaatt tgagtaagtt ctcaattttt ctttatgaat
    13801 gaaaattttg gcacgcaaat ttgcgtaaat aaccggcaaa tttacgtgcc ttttgtgagc
    13861 ttgctcgcac ttcgccccgc gtcaccctac ggcgatgcga aggttaattc ttacgaccgg
    13921 taatacggca ccactcttct ttttccacga ccgggtccag tgcgaagcta tcggcataag
    13981 cttcacaaac gctctctgcc tggcttgcca gaataccgga aaggcccagc aaaccgcctg
    14041 aaaccggcag gacgctgatt aacggtgcca gttcacgtaa tgggcctgca aggatgttag
    14101 cgaccaccac gtcggctttc atttcttctg gctgatcttt cggtaagtag agttccagac
    14161 ggtcagaaac gccattacgt tcggcgttgt cgcggctggc ctgaatcgcc tgcggatcga
    14221 tatcaatacc aatggctttt gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg
    14281 aaccacagcc aaagtcgatg actgttttac cggttaaatc gaggctgtcg agccattgca
    14341 ggcacagaga ggtggttgga tgggtacccg taccaaacgc cagccctgga tctaacatca
    14401 cgttgacggc gttttcgtcc ggcacatcac gccagctagg gcagatccac agtcgttcac
    14461 caaagcgcat cgggtggaaa ttatccatcc attcgcgctc ccagtcttta tcttctagtt
    14521 gttcgatttt atgcgcgaag cctgcgccga gcagcggatg gttttccaga atcgccacca
    14581 cgtcgttcat atcggtttca gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc
    14641 gcgtttcgcc cggcagcggc tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag
    14701 aaacggcacc cgcttccatc agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt
    14761 tcagtttcag ttggatccaa ggcatggcaa aactctttat ttatcagtag tcaaaacggt
    14821 agcttgcggg acggatgtac cgaaacggtt tccgaccagg aaagccagca aacttagtag
    14881 taacgagggc acaatagggt ggaagcccag gtactgaata ttcagcgtcg cgagtacggc
    14941 atacagcacg ccgccaacga tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca
    15001 gtaaagaccc agcaccagcg gccacaggaa aacggcttcc agcccaccga aggctagcaa
    15061 attcagccag atgatcattt ctggcggctt ccaggcggca agcagcagca acgcgccgag
    15121 aactaacgta attaccgccg acatccgctt cagacgcgtc tcgttttgca tttgatccgg
    15181 acggatattc agatagagat ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt
    15241 aattgtcgac atgatcgcag ccataggtgc agccagaaag atcccggcag caaaaggtgg
    15301 cagcactttt accattaacg tcgggatcac cagatccggt acggtgagat cggggatcac
    15361 tgcatgacct aacgctccgg ccaggtgcat accgaacatc aggattgcga ctacaattgt
    15421 accgatgatg atcccccggt gtactgcttt gctgtcttta taagagatac agcgcaccgc
    15481 agtatgcggc aggccaatca cgccaaagca caccagtacc cagaacgacg tcataaaggc
    15541 aggcgacaga atatcgtcag cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa
    15601 ggtctgtact gcgttgctta agccgccagc ggcatgtacc acgccaataa gcagcacaac
    15661 ggtgccaatc agcatcacaa gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc
    15721 accaaaggcg gtatataacg caatgctgat accaaaaatc agcagcccgg tttcataagg
    15781 aatacccgcc gcggtttcca gcaggcgcgc accgccgata aactgcacgg tcatcgcacc
    15841 aacgaacgca accagcaaac tcaaactcgc cagccacacc agaagacgac tctggtagcg
    15901 ggcaaacagc atgtcgttca gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt
    15961 cttgccgaga ataccgagtg aaagccagac tgcaggaagc tgaatcatcg ccagcaatac
    16021 ccagcccagc ccgtatttat aagcagctcc tggcccgccg ataaacgaac tggcactgat
    16081 ataggtcgcg gtgagcgtca tcgccagcac aataccgccc atagagcggc tgccgaggaa
    16141 atactcatta aggaaggtgc cggtgctccg tttacgcatc gcataaaccg agataccgaa
    16201 caccaccacc agataggcga ccagcggtag aattacttca agctgcatcg tcatcctcca
    16261 gtgggatatc gcgatagata aatttcacca tcgcccagca cagtccaata aacagtagcg
    16321 gcgtcaggat gcaggccatc tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc
    16381 caggtaagta agcggctact aaccaaactg ccagatacaa aagggtcagc cccagcgccc
    16441 agcgcgcctc tttatgggcc tgaacaaaac gagtgtccat tttttgtccc tgatgggtga
    16501 agaaagcgag gattgtacct tatgggggtt gtcgatcccc agtaataaaa aaggccggaa
    16561 aatccggcct tttgacgctt ttgcagtctt atttttcctg aagaccgagt tttttctcca
    16621 gatagtggat gttagtgcca ccatgctgga agttctcgtc attcatgatg cggatctgca
    16681 gatcaacgtt ggttttgata ccgtcgatga tcagctcctg cagcgcattc ttcatgcgag
    16741 caatcgccac gtcacggttt tcaccgtagc aaatcagctt accgatcatt gagtcatagt
    16801 acggcggtac ggtgtagccc gcgtagatat gagactccca acgtacgcca aaaccgccag
    16861 gtgcgtggaa acgggtgatt ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt
    16921 tgatacgaca ttccaccgca tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg
    16981 gttgaccggc agcgatacgc agctgttctt tgatcaggtc aacgccggtg atcatttctg
    17041 taaccgggtg ttctacctga atacgggtgt tcatttcgat gaaatagaac tcgccgtttt
    17101 cgaacaggaa ctcgaaagta cctgcaccgc gatagccgat atcaacacac gctttagcgc
    17161 aacgttcgcc gatgtagcga cgcagttccg gggtaatgcc cggtgctggt gcttcttcga
    17221 ccactttctg gtggcggcgc tgcatggagc agtcacgttc cgccagatag atagcgttgc
    17281 cctgaccgtc agccagtacc tgaatctcga cgtggcgagg attttccagg tatttctcca
    17341 tgtaaaccat atcgttgctg aaagcagctt tcgcttccgc acgggtcatg gagatggatt
    17401 gtgccagttc agcgtcgccg cgcactacgc gcataccgcg accaccgccg ccgccggagg
    17461 ctttgataat caccggataa ccaatgcgtt tagcaatggc acggttttta tccatatcgt
    17521 cgcccaacgg gccgtcagaa cccggtacgc aagggacgcc cgcttttttc atcgccgcga
    17581 ttgcggatac tttgtcgccc atcaggcgaa tggtttctgc tttcgggccg atgaagataa
    17641 agccggagcg ttcaacctgc tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg
    17701 gatggattgc tactgcgccg gtgatttcag cggcgctgat gattgccggg atgttcagat
    17761 aactttttac tgacggagca gggccaatac agaccgtttc atctgccagt aatacgtgtt
    17821 ttagatcgcg atccgcgctg gagtgcacag cgacagtctt gatgcccagt tctttacagg
    17881 cacgaagaat acgcaatgca atctcgccgc ggttggcaat aacaatttta tccagcatgt
    17941 tcgcctcgtt actcgatgac gaccagcggc tcgtcaaatt ctaccggttg tccactttcg
    18001 accagaattg ctttcacggt accggatttg tccgcttcga tctggttcat cattttcatg
    18061 gcttcaacga tgcacagggt atcgcccacg ttgactttct gacccacttc gatgaacgct
    18121 tttgcgtccg ggcttggggt gcggtagaaa gtaccaacca tcggggaacg tacgatgtga
    18181 ccactgattt ccgctgctgc tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg
    18241 ttagattgag ctggctgctg catcattggt gcagcgtaag cttgttgcat cacagggaaa
    18301 cttgcggcag gagctgcacg gctaatgcgt actgactctt cgccttcaga aatttccagt
    18361 tcggagatgc ctgattcttc aaccagctcg atcagttttt taatcttacg aatatccatg
    18421 agtgggttcc gtactctttg tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa
    18481 agcgtatgcc ccgacagctg tatgcatagc gataaattcc agcaggccgg gacgccggtc
    18541 tattttgccc cagagtgtca atgttagact tgacggacat tgtgcagcga tctgctgcta
    18601 tcctccggca aaaaacaaaa tataccttcg ttgcgccaat gtcacctttt ccgcagcgca
    18661 aaaactgcgt cagggaggac gaggtcgcac attataacga tttcgtagca attggcagct
    18721 aaatactggt cttatcaggg aagataatca acagctaaca tgtaaataac cttcaacacc
    18781 gtgtaatttg caacaagccg cacaattcac gaaattagcg ccatcatcga cggaacttct
    18841 tataacgtaa ggtgaaaagc tgtaaagcca gccctgcgaa gatgaggggc tgcaatgaga
    18901 taatcttcac agaccacaaa taatgtaggg atgccaggat cgcgacaaga tagacgacat
    18961 tacgcagcgg ttgctgggga ttacccaatt ttcactgcat cgtctggata gatgtgaatg
    19021 ctaaagcacg caaaatcaac cgactgacaa ttctcagcat taaataggtt cgagtggtta
    19081 atttgtagtc aaataaaatc agattattca ccccatggga ccagaaatca gtaacaggtt
    19141 aagcgtagtg tcgcccaggc aaagcaccat aaacctaaca ggcgacggag gcatatcaat
    19201 aatggctgtt tagaggagta tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt
    19261 tccagtttgg atgatgttgt acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc
    19321 taaccgcttt tcccgttaac cgattgaata atgatgtcaa aatgagttcc ctctcttatt
    19381 attccttgct aaatggttag ttaaccttca ccagcgtgcg accctggatc tggttattaa
    19441 tgatggcctc ggcaaagttc ggtgcctctg acagagatat ctcttttgcc gcctgggtat
    19501 agaatgattc cggtaaatcg gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg
    19561 gcgtcattac tgaatccacc ccttgcaaac ggacattacg cagaataaat ggcatgaccg
    19621 tggttggcag agtaaaacca cccgccagac cacaggccgc cacgcagccg ccgtaattca
    19681 tttgcgccag cacttttgcc agcactttgt cgccaacagt gtcaattgcc ccagcccaga
    19741 cttgtttttc cagaggacgg gattcggcaa actcatcacg agggagaata cgactggcac
    19801 ctaaactttt cagatattca tgggtacttt cgcgaccgga aacggcaacg acctgataac
    19861 ccaacttatg cagcagcgcc acggcggtac tgccgacgcc accactggca cccgtcacga
    19921 caatctcccc gtcctgcggg cgaacaccgg catcttccag cgccatcaca cacagcatgg
    19981 cggtaaaacc ggcagtaccg ataatcattg ctttacgcgc gtccagccct tgcggcatgg
    20041 caaccagcca gtcacctttc actcgcgcct gctccgccag cccaccccag tggttttcac
    20101 caacgcccca gccagtgagt aacacctcct gaccggcatg aaaacgcgga tcttcgctgg
    20161 tgcgtacagt tccggcaaaa tcgatcccag gaatcatcgg aaaattacgg atgatttttc
    20221 ccttaccggt aatcgccagc gcatctttat agttcaggct cgaccagtga acatcaaccg
    20281 tgacatcgcc ctccggcagg cgactttcgt caagtgtctg taccgatgcg agagttttgc
    20341 cgtcctgctg ttctaaaagt aacgcctgca taagtggtcc tcatgtgcat gatggattgg
    20401 aaaataattt ctgaagacta tactcgctaa tggaaaagca aagccgatga agcgcaataa
    20461 attgcgagat aaatctgatt tgctagtatg cccgcttcct cactatcgga gttaacacaa
    20521 ggatgagatt aacgacgaaa ttttcggcct ttgttacgct gctcaccggg ttaacaattt
    20581 ttgtgacttt gctgggctgt tcgctaagtt tctacaacgc aattcagtat aagtttagtc
    20641 atcgcgttca ggcggtggcg acggcgattg atacccacct tgtgtcgaat gacttcagca
    20701 cattaaggcc acaaattacc gaattaatga tgtcggcaga tatcgttcgt gtagacctgc
    20761 tccatggtga taaacaggtt tataccctgg ccagaaatgg tagttatcgt ccggttggct
    20821 ccagcgatct gtttcgcgaa ctgagtgttc cgttgataaa gcatccgggg atgtcgctgc
    20881 gtctggttta tcaggatccg atgggcaact atttccattc tttaatgact accgcgccgc
    20941 tcacgggggc gattggcttt atcattgtta tgctctttct ggcggtacgc tggttacaac
    21001 ggcaacttgc cgggcaagaa ttgctggaaa cccgggctac tcgtatctta aacggtgagc
    21061 gtggctctaa tgtgttggga accatctatg aatggccgcc cagaaccagc agtgcgctag
    21121 atacgctgct tcgtgaaatt cagaacgcac gcgaacaaca cagccgtctt gatacgctga
    21181 tccgctctta tgccgcccag gacgtgaaaa ccggcctcaa taaccgactc tttttcgata
    21241 atcagttagc aacgttactg gaagatcagg agaaagtagg tacccacggg atcgtgatga
    21301 tgattcgtct gccggatttc aatatgttga gcgatacttg ggggcacagc caggttgaag
    21361 aacagttctt cactctgacg aatctgctgt cgacatttat gatgcgctac cctggcgcac
    21421 tgctggcgcg ttaccaccgc agtgattttg ctgcgctgtt accgcaccgg acgttaaaag
    21481 aggcagagag catcgccggt cagttaatca aagccgtcga taccttgccg aacaataaaa
    21541 tgctcgatcg cgacgatatg atccacattg gtatctgcgc ctggcgtagt ggtcaggata
    21601 ccgagcaggt aatggaacat gcagagtctg ccacgcgtaa tgcgggattg cagggcggca
    21661 atagctgggc tatttacgat gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc
    21721 gtacgcttat cgagcaaatg ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg
    21781 ttactcgcga aggtcaggtt catcatcgcg aactcatgtg ccgcatcttc gatggtaatg
    21841 aagaggttag ctcggcggag tatatgccga tggtcttgca gtttggctta tcggaagagt
    21901 atgaccgtct gcaaatcagc cgtcttattc cactattgcg ttactggcca gaggaaaatc
    21961 tggcgattca ggttaccgtt gagtcgctga ttcgcccgcg ttttcagcgt tggctgcgcg
    22021 atacgttaat gcaatgtgaa aaatcacaac gaaaacgcat aattattgaa cttgcagagg
    22081 ccgatgtagg tcaacatatc agtcgtttac aacctgttat tcgtttagtg aatgctttag
    22141 gggtacgggt agccgtcaac caggctggtt tgacgctggt aagcaccagt tggatcaaag
    22201 aacttaatgt tgagttactc aagctccatc cggggctggt cagaaacatt gagaagcgaa
    22261 cggagaacca gctgctggtt caaagcctgg tggaagcctg ctccgggacc agcacccaag
    22321 tttacgccac cggtgtgcgt tcgcgaagcg agtggcagac cctgattcag cgcggtgtta
    22381 caggcgggca aggggatttt ttcgcgtcct cacagccact tgatactaac gtgaaaaaat
    22441 attcacaaag atactcggtt taacctgccg tttaatccgt tttcacgtag aataacgcgc
    22501 gctgcgtctc atgggagtgt gcttgtctgc tcgccagatt gttgcagcac atatgcagat
    22561 gaatgacctt acgcggttgc aaacaggcga ggaatgctgc tgatgcatta agcctttctg
    22621 gactcaggca gagatttgta acaaaggaaa cgaactgcac taattttcac cgtagcagat
    22681 gatttttgcg ccttgtcgct gctgcgtgtg gttggtaaag taagcggatt ttcttttccg
    22741 ccccagcttt caggattatc ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat
    22801 gacttgtcca ttgacctggg tactgcgaat accctcattt atgtaaaagg acaaggcatc
    22861 gtattgaatg agccttccgt ggtggccatt cgtcaggatc gtgccggttc accgaaaagc
    22921 gtagctgcag taggtcatga cgcgaagcag atgctgggcc gtacgccggg caatattgct
    22981 gccattcgcc caatgaaaga cggcgttatc gccgacttct tcgtgactga aaaaatgctc
    23041 cagcacttca tcaaacaagt gcacagcaac agctttatgc gtccaagccc gcgcgttctg
    23101 gtttgtgtac cggttggcgc gacccaggtt gaacgccgcg caattcgtga atccgcgcag
    23161 ggcgctggtg cccgtgaagt cttcctgatt gaagaaccga tggctgccgc aattggtgct
    23221 ggcctgccgg tttctgaagc gaccggttct atggtggttg atatcggtgg tggtaccact
    23281 gaagttgctg ttatctcctt gaacggcgtg gtttactcct cttccgtgcg cattggtggt
    23341 gatcgtttcg acgaagctat catcaactat gtgcgtcgta attacggttc tctgatcggt
    23401 gaagccaccg cagaacgtat caagcacgaa atcggttcgg cttacccggg cgatgaagtc
    23461 cgtgaaatcg aagttcgtgg ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg
    23521 aactccaacg aaatcctcga agcactgcag gaaccgctaa ccggtattgt gagcgcggta
    23581 atggttgcac tggaacagtg cccgccggaa ctggcttccg acatctccga gcgcggcatg
    23641 gtgctcaccg gtggtggcgc actgctgcgt aaccttgacc gtttgttaat ggaagaaacc
    23701 ggcattccag tcgttgttgc tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa
    23761 gcgctggaaa tgatcgacat gcacggcggc gacctgttca gcgaagagta atcggatgca
    23821 ggcaggggaa atgtctgttt accctgcctg gtctgatacg agaatacgca taacttatga
    23881 agccaatttt tagccgtggc ccgtcgctac agattcgcct tattctggcg gtgctggtgg
    23941 cgctcggcat tattattgcc gacagccgcc tggggacgtt cagtcaaatc cgtacttata
    24001 tggataccgc cgtcagtcct ttctactttg tttccaatgc tcctcgtgaa ttgctggatg
    24061 gcgtatcgca gacgctggcc tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc
    24121 aggaactgtt gctgaaaaac agtgaactgc tgatgcttgg acaatacaaa caggagaacg
    24181 cgcgtctgcg cgagctgctg ggttccccgc tgcgtcagga tgagcagaaa atggtgactc
    24241 aggttatctc cacggttaac gatccttata gcgatcaagt tgttatcgat aaaggtagcg
    24301 ttaatggcgt ttatgaaggc cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg
    24361 tggccgtcgc taaactgacc agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc
    24421 caatccaggt gctgcgcaac gatatccgcg taattgcagc cggtaacggt tgtacggatg
    24481 atttgcagct tgagcatctg ccagcgaata cggatattcg tgttggtgat gtgctggtga
    24541 cctccggtct gggcggtcgt ttcccggaag gctatccggt cgcggttgtc tcttccgtaa
    24601 aactcgatac ccagcgcgct tatactgtga ttcaggcgcg tccgactgca ggactgcaac
    24661 gtttgcgtta tctgctgctg ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc
    24721 cggaagaggt gcatcgtgtt gctaatgaac gtctgatgca gatgatgccg caggtattgc
    24781 cttcgccaga cgcgatgggg ccaaagttac ctgaaccggc aacggggatc gctcagccga
    24841 ctccgcagca accgacgaca ggaaatgcag ctactgcgcc tgctgcgccc acacagcctg
    24901 ctgctaatcg ctctccacaa agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc
    24961 gtgcgccggg agggcaatag tggcgagcta tcgtagccag ggacgttggg taatctggct
    25021 ctctttcctc attgcgctgt tgctgcaaat catgccctgg ccggataacc tgattgtttt
    25081 ccggccaaac tgggtgttac tcatcttgtt gtattggatc ctggccttgc ctcatcgcgt
    25141 aaatgtgggc acaggttttg tgatgggtgc catactggat ctgatcagcg gctcgacgct
    25201 tggcgtacgc gtattggcga tgagcatcat tgcttacctg gtggcgctga aataccagct
    25261 tttccgcaac ctcgcattat ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt
    25321 ggatattatt gttttctggg cagagttttt agtgattaac gtctctttca gaccggaagt
    25381 gttctggagt agtgtagtca atggggtgct ctggccgtgg attttcttgc tgatgcgtaa
    25441 agtccgtcag cagtttgcag tgcaataaag gtttctatga cttctctgta tttagcttcc
    25501 ggttctccgc gtcgtcagga gttacttgcg caacttggcg tgacctttga acgtattgtt
    25561 acgggcattg aggagcagcg tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg
    25621 gcgcgcgaga aagcacaggc gggtgtcgcg caaacggcgc aggatctccc ggtgctgggt
    25681 gcggatacta tcgttatcct gaatggagaa gtgctggaga aaccgcgcga cgcagagcat
    25741 gcggcgcaga tgttgcgcaa attatcgggt cagacccatc aggtgatgac ggcagtggcg
    25801 ttggccgaca gccagcacat tctcgattgc ctggtggtca ccgatgtgac tttcagaacg
    25861 ttaacagacg aagacatcgc gggctatgtc gccagcggtg aaccgttaga taaagcaggt
    25921 gcatacggta ttcaggggct gggtggctgt tttgtcagga agataaatgg cagctatcac
    25981 gccgtagtcg gcttaccgct ggttgaaacg tatgaattat taagtaattt taacgcactg
    26041 cgtgagaaaa gggataaaca tgacggctga attgttagta aacgtaacgc cttcggaaac
    26101 gcgagtggcg tatattgatg gcggtattct gcaggaaatt catattgaac gtgaggcgcg
    26161 acgcggaata gtaggcaata tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca
    26221 ggcggctttt gtagatattg ggctggataa agccgcgttt cttcatgcat ccgatatcat
    26281 gccgcacacc gaatgtgtgg cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc
    26341 ggaactggtt cgtcaggggc aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac
    26401 taaaggtgcg cgcctgacca ccgatatcac gctgccttct cgctatctgg tctttatgcc
    26461 aggggcttct cacgttgggg tttcccaacg tattgaaagc gaatcagaac gtgaacgcct
    26521 gaaaaaagtg gtcgcagagt attgcgacga gcagggcggg tttatcatcc gtaccgcagc
    26581 ggaaggggtt ggcgaggctg aactggcctc cgatgccgct tatctgaaac gcgtctggac
    26641 caaagttatg gagcgtaaaa aacgcccgca gacccgttat cagctgtacg gcgaactggc
    26701 gctggcgcag cgtgttctgc gtgatttcgc cgatgccgaa ctggaccgca ttcgcgttga
    26761 ctcacgcctg acttacgaag cattgctgga gtttacctcg gagtacattc ccgagatgac
    26821 cagcaagctg gagcattaca ccggacgcca gccgattttc gatctctttg atgtcgaaaa
    26881 cgaaatccag cgagcgctgg aacgcaaagt agaactgaaa tccggtggtt atctgattat
    26941 cgaccagacc gaagcgatga ccaccgtgga catcaatacc ggggcgtttg tcggtcatcg
    27001 caatctggac gacaccattt tcaataccaa tattgaagcg acgcaggcta tcgctcgcca
    27061 gttacggttg cgcaatctgg gcgggattat cattattgat ttcatcgata tgaataatga
    27121 agatcaccgc cgacgcgtgc tgcactcgct ggagcaggca ttgagcaaag accgggtgaa
    27181 aaccagcgtt aatggttttt cggcgctggg gctggtggag atgacgcgta aacgcacccg
    27241 cgaaagcatt gagcacgtac tgtgtaacga atgcccaacc tgccacggtc gcggaacggt
    27301 gaaaaccgtg gaaacggtat gctatgaaat catgcgcgag attgttcgtg tccaccatgc
    27361 ttacgactcc gaccgtttcc tggtctatgc ttctccggca gtagctgaag ccttgaaagg
    27421 cgaagagtca cactcgctgg cggaagtgga aattttcgtt ggtaaacagg ttaaagtaca
    27481 aattgaaccg ctctataacc aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg
    27541 gccgccatcc ggcaaagggt ttttgagtca catttttagc agacaaggag tgacgggtga
    27601 ggcgattgcc ggggatttta ctgcttactg gagccgcgct cgttgtgatc gctgccctgc
    27661 tggttagcgg cctgcgtatt gctttaccgc atcttgacgc ctggcgtccg gaaatcctca
    27721 acaaaataga atccgcgact ggcatgccgg tagaagccag tcagctctca gccagctggc
    27781 agaattttgg cccgacgctt gaagcacacg acatccgtgc agaactaaaa gatggcggcg
    27841 aattttcggt taaacgcgtt actctggcgc tggatgtctg gcagagcttg ttacatatgc
    27901 gctggcagtt tcgcgacctc actttctggc agctgcgctt tcgcaccaac actcctatca
    27961 ccagcggtgg tggtaatgat agcctggaag ccagtcacat cagcgatctg tttcttcgtc
    28021 aatttgacca tttcgatctc cgcgacagtg aagtcagttt tctgacgcca tccggtcagc
    28081 gcgccgagct ggcgatccca caactcacct ggctgaacga tccacgtcga caccgtgcgg
    28141 aaggcctggt aagcctctcc agccttaccg gacagcacgg cgtgatgcag gtgcgcatgg
    28201 atttgcgcga tgatgagggg ttgttaagca atggtcgcgt ctggctacag gcggatgaca
    28261 tcgacctgaa gccgtggctc ggtaaatgga tgcaggacaa tattgctctg gaaacggcac
    28321 agttctccct tgaaggctgg atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct
    28381 ggctgaaaca gggcggtgcc agctggttgg gcgagaagca aacgcatacg ctgtcggtgg
    28441 ataatctgac cgcgcatatt acgcgtgaaa atccgggctg gcagttctct attcccgata
    28501 cacggatcac gatggacggc aaaccctggc cgagcggagc attgacgctg gcctggatac
    28561 cggaacagga cgttggcggc aaagacaata aacgcagtga cgaactccgg attcgcgcca
    28621 gtaatctgga gctggcaggc ctggagggca tacgcccgct ggccgcgaaa ctttcacctg
    28681 cactgggtga tgtttggcgc tccacacaac cgagcggcaa gattaacact ctggcgctgg
    28741 atatcccgct tcaggcggca gacaagaccc gttttcaggc atcgtggagc gatctggcct
    28801 ggaagcaatg gaaattatta ccgggtgcgg aacacttctc cgggacgctt tccggcagcg
    28861 ttgaaaatgg tttgcttacc gcgtcgatga agcaggcaaa gatgccttac gaaacggtat
    28921 tccgtgcgcc actggaaatc gccgacggcc aggcaactat aagctggctg aacaatgaca
    28981 aaggtttcca gctggatggg cgtaatattg acgttaaagc caaagccgtc catgcgcgcg
    29041 gcggttttcg ttacctgcaa cctgctaacg atgaaccctg gctgggtatt ctggctggca
    29101 tcagtaccga tgatggttca caagcctggc gctatttccc ggaaaacttg atgggtaaag
    29161 acctggttga ttacttaagt ggcgcgattc agggcggtga agcggataac gcgacgctgg
    29221 tttatggtgg caatccgcaa ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc
    29281 tggtgccgct gcgcaacgcg aagtttgcct tccagccgga ctggcctgca ttaactaacc
    29341 ttgatattga actggacttt attaacgacg gtttatggat gaaaaccgat ggcgttaatc
    29401 tgggcggcgt gcgcgcgagt aatctcaccg cagtgatccc tgactactcg aaagaaaaac
    29461 tgctgattga cgctgacatt aaaggtccgg gtaaagccgt tggcccttac tttgatgaga
    29521 caccgctgaa agattctctg ggggcgaccc tgcaagaact ccagctcgac ggcgatgtga
    29581 atgctcgctt acatcttgat atcccgctga acggcgaact ggtaaccgcg aaaggtgaag
    29641 tgacgctgcg taataacagt ctgtttatca aaccactcga cagcaccctg aaaaatttga
    29701 gcggtaaatt cagctttatc aatagcgatc tgcaaagtga accactgaca gcaagctggt
    29761 ttaatcagcc gttgaacgtg gatttttcca ccaaagaagg ggcaaaagcc taccaggtag
    29821 cggtaaacct caacggtaac tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga
    29881 acgaagcatt gagtggcagc gtggcgtggg atggtaaagt gggcattgat ctgccttatc
    29941 atgctggtgc gacctataac atagagctga acggcgatct aaagaatgtg agcagtcact
    30001 taccttcacc gttagccaaa cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg
    30061 gcaatctcaa cagctttgaa ttaaccggac aggctggtgc ggataatcat ttcaatagcc
    30121 gctggttgct cggtcaaaag ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa
    30181 cgctcccgcc gttgccggaa caaagtggcg ttgaactcaa tatgccgccg atgaatggtg
    30241 ccgagtggct ggccctgttc cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt
    30301 tcccacaaca cataacgtta cgtacgccta tgttgtcact gggaaatcag caatggaata
    30361 acctgagtat tgtttcgcaa ccgacggcaa atggcaccct ggttgaagcg caagggcgtg
    30421 aaatcaacgc cacgctagcg atgcgtaata acgcgccgtg gctggcgaat atcaaatatc
    30481 tttattacaa cccgagcgtg gcgaaaactc gtggtgattc aacgccgtca tcacctttcc
    30541 cgacaacgga gcgcattaac ttccgtggct ggccggacgc ccaaatacga tgcacagagt
    30601 gctggttctg ggggcaaaaa ttcggtcgca ttgacagtga tatcaccatt tctggcgata
    30661 cgttaacgct gaccaatgga ctgattgata ctggtttctc gcggcttact gccgatggtg
    30721 aatgggttaa taatccgggg aatgaacgta cctcgctgaa aggaaaactg cgcgggcaga
    30781 aaattgatgc cgccgcagaa ttttttggtg tcacgacgcc catacgccag tcgtcattta
    30841 atgtggatta cgatttacac tggcgcaaag caccctggca gccagatgaa gcgacgttga
    30901 atggcatcat tcatactcaa ctgggtaaag gcgaaattac cgaaatcaat accggacatg
    30961 ccgggcaatt gctgcgctta ttgagcgtag atgccctgat gcgtaagctg cgttttgatt
    31021 tcagagacac ttttggcgaa gggttctatt ttgactccat tcgcagcacc gcgtggatta
    31081 aagacggcgt tatgcacacc gacgacacgc tggtggatgg cctggaggcg gatatcgcca
    31141 tgaaagggtc ggtaaatctg gtacgtcgcg acctgaatat ggaagcggtt gtcgcaccag
    31201 agatttctgc gacggtgggc gtggctgcgg cttttgcggt taaccccatt gttggcgcgg
    31261 cagtgtttgc cgccagtaaa gtgctggggc cgctgtggag caaagtctcc attttgcgct
    31321 atcacatttc gggtccgctg gacgatccgc aaatcaacga agtgttgcgc caaccgcgta
    31381 aagaaaaagc gcaatgattt gacgagggcg cgtaattgcc ccaatctcat aggataatcg
    31441 ttgccaaagg ccaacgagcc agaacataac cgtaggtcgg atagggcgtt
//
LOCUS       DAEVZA010000045        30784 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEVZA010000045 DAEVZA010000000
VERSION     DAEVZA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.045"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            248..631
                     /gene="secE"
                     /locus_tag="LAF92_002136"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="LAF92_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBI9791140.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="LAF92_002137"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="LAF92_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBI9791141.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="LAF92_002138"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="LAF92_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBI9791142.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="LAF92_002139"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="LAF92_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBI9791143.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="LAF92_002140"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="LAF92_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBI9791144.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="LAF92_002141"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="LAF92_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBI9791145.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="LAF92_002142"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="LAF92_002142"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBI9791146.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="LAF92_002143"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="LAF92_002143"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBI9791147.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13215
                     /locus_tag="LAF92_002144"
     CDS             12676..13215
                     /locus_tag="LAF92_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HBI9791148.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="LAF92_002145"
     CDS             complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="LAF92_002145"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBI9791149.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="LAF92_002146"
     CDS             complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="LAF92_002146"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBI9791150.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="LAF92_002147"
     CDS             complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="LAF92_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBI9791151.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="LAF92_002148"
     CDS             complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="LAF92_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBI9791152.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="LAF92_002149"
     CDS             complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="LAF92_002149"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBI9791153.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="LAF92_002150"
     CDS             complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="LAF92_002150"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBI9791154.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19070..19170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="LAF92_002151"
     CDS             complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="LAF92_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBI9791155.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19793..20566
                     /gene="nudC"
                     /locus_tag="LAF92_002152"
     CDS             19793..20566
                     /gene="nudC"
                     /locus_tag="LAF92_002152"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBI9791156.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20606..21670
                     /gene="hemE"
                     /locus_tag="LAF92_002153"
     CDS             20606..21670
                     /gene="hemE"
                     /locus_tag="LAF92_002153"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBI9791157.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21680..22351
                     /gene="nfi"
                     /locus_tag="LAF92_002154"
     CDS             21680..22351
                     /gene="nfi"
                     /locus_tag="LAF92_002154"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBI9791158.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22394..22984
                     /locus_tag="LAF92_002155"
     CDS             22394..22984
                     /locus_tag="LAF92_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBI9791159.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23171..23443
                     /gene="hupA"
                     /locus_tag="LAF92_002156"
     CDS             23171..23443
                     /gene="hupA"
                     /locus_tag="LAF92_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBI9791160.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23456..24151
                     /locus_tag="LAF92_002157"
     CDS             23456..24151
                     /locus_tag="LAF92_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBI9791161.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="LAF92_002158"
     CDS             complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="LAF92_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBI9791162.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24816..26192
                     /gene="zraS"
                     /locus_tag="LAF92_002159"
     CDS             24816..26192
                     /gene="zraS"
                     /locus_tag="LAF92_002159"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBI9791163.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26189..27514
                     /gene="zraR"
                     /locus_tag="LAF92_002160"
     CDS             26189..27514
                     /gene="zraR"
                     /locus_tag="LAF92_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBI9791164.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGHFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27511..28800)
                     /gene="purD"
                     /locus_tag="LAF92_002161"
     CDS             complement(27511..28800)
                     /gene="purD"
                     /locus_tag="LAF92_002161"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBI9791165.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28812..30401)
                     /gene="purH"
                     /locus_tag="LAF92_002162"
     CDS             complement(28812..30401)
                     /gene="purH"
                     /locus_tag="LAF92_002162"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBI9791166.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7272 a         7987 c         8410 g         7115 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctagaaga
    10861 gaactctgtc gacgcggtta aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca agggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaatactg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggtca gaccattact cgtcagacag acgaattgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagca cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggggcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt
    12541 cagaatattt aaggaatttc tgaatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaaactcgg caaggattcg atactattcc tgtgtaactt
    12661 tcttaaggaa cgagaatgaa acaggaagtg gaaaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttt tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac gaacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcacacactt aaaaagaact
    12961 attttggcgc tgccagaaag caacgttatt catgccgggt atggtagcta tgccgtgatt
    13021 gaggtggact tagacggagg agataaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aactccgttt gcatgtaact agcgcttatc ccatttctga aaaacagaaa
    13141 ggtaaatcag tgaaattttt caccattgcc tacaacttat tgagaaataa gcagcttcct
    13201 cagccctcaa aataacaaaa cccaccttaa ggtgggtttc gccagagaat tatctctggt
    13261 attcagaacg ccattaccgg actttgcctt gaccttgcga taatcgcagg ttgcgggatg
    13321 tctgaatttc ttcagtctgc tgcatcctgg aagatgagaa catgtgttct tattttcgtc
    13381 tctatcatag ttgagtattt actctcttac aatcagatct ctttcattgc tcaacaggcg
    13441 atggcttcag actttgcatt acggaatttt taagaaaggc agggcgaaac gaggaagaag
    13501 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca gaacgctttc
    13561 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13621 cgtctcatag tctttgcgag gcccgtccca gatagctgtc ccagtctttc cataccggct
    13681 gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg
    13741 agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt ttcgagaagg
    13801 cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg
    13861 tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta
    13921 actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg cgcaggcgcg
    13981 gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc aaatgttcgg
    14041 caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg ccgaggccta
    14101 tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag aagaagtcct
    14161 gttttttgcc tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa
    14221 ccatcacgcc atccagacca agctgcttta actcggcgta ttccgtctcc gccagcggtt
    14281 gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga cgacgaaagt
    14341 aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt tcaaagccca
    14401 tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14461 tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14521 aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga cgctgcgccc
    14581 gttgggccag ttgttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt
    14641 cgcgggtgaa ttgcgaggca tttagcgccc gctctacgtc aacagccgtt ttgccgttga
    14701 tacgcaggcg gatgtcatcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc
    14761 cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc
    14821 cggtccggac tgacgtgcca gcaggcctgc ttcaaccgcc agacgaaatg ccttcgccat
    14881 gttgacggga tcgtccgcta cggcaatcgc cgtattcact aacaccgcgt cggcccccat
    14941 ttccagggcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggaac
    15001 tgtggcctgc tggataataa tctccagcat ggcacaggtt tccagtccct gattcgagcc
    15061 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    15121 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcatttcggc
    15181 ggctttcaga gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa
    15241 ttttagccag ttagtgccta acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc
    15301 cgctgttttc gccccggatg tatttggcag cagggtcaca cccgccgcga taagcggttc
    15361 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg
    15421 gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc
    15481 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatttcaa ccccctgcaa
    15541 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac
    15601 gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct
    15661 ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga
    15721 tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc
    15781 cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc tataccgctt
    15841 aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc
    15901 acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag gcagcggtaa
    15961 cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg
    16021 gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg gcgagtcgcc
    16081 atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt taacgcctca
    16141 cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc
    16201 tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag gatttgtcgt
    16261 tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg
    16321 cccgccaggt acagcgcagc aggtgtaccc agcccgccca gaccgataat cagcacctgg
    16381 ctgtcgagca gtttttgctg cccgtccaga gcgatatcgt cgagcaggat ttggcggcta
    16441 taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16501 tgccaaacgc cagtctgcag cttgagtaat ggcgctgacg acggcgatac tgccgacacc
    16561 cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca cggtgggata
    16621 atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat
    16681 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16741 cgcgacgtcg atttccatat cgtcatgagt cgaaacgccc agccgcaggc ctgccgcgcg
    16801 gatggcattg agatcggtgg cttgcaaatc ttcctgccct aaatggacgc catacgcctg
    16861 atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16921 cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16981 tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac
    17041 caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat acatcacgct
    17101 tcctccttac gcaggtagat ttctccgcct ctggcacgga agttctccga catatccgcc
    17161 attcccattt caatagtttg cgtggcggcg taatcacgca cttcctggct gattttcatc
    17221 gaacagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga ctcttgcggc
    17281 agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct
    17341 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17401 tgccctttcg ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta
    17461 acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17521 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc atagcccggc
    17581 gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17641 tcctcggtca tattgcggcg gatcatctgc atcggcacgt gacctgggcc ttcaatcatc
    17701 acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg cagctcggca
    17761 aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc gcccagcgac
    17821 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17881 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17941 ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg
    18001 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    18061 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    18121 ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    18181 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    18241 tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac caggaaattg
    18301 cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcacgt
    18361 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag atgtgcgcca
    18421 aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc gcggcccata
    18481 ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc
    18541 agttgggtga cacggcgtcc tgctttggcg cgttttggtg ttagtacacc gctaaaacgc
    18601 agttcgtcga ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta
    18661 agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagcccttgc
    18721 tgcacgttaa tggcaatctg cggatcacca tacgggccgg aggtgtcgta gaccggaatc
    18781 gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt cgggctaagc
    18841 tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt
    18901 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt
    18961 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag gacgtggctt
    19021 gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata gcagattaac
    19081 tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct
    19141 cagccagtta aggcactccg acaagaaatt agccccgcaa atggggcatt gaatgtaaat
    19201 tacgcgttaa cagcacagaa ctcaagcagg atgtttgacg cgggcggcgt caagcaccag
    19261 cagaatcagc ttatcttcca gcacaaagcg cgcttccagc gcctcgccga tgtcagataa
    19321 aacctgttga aactcaaggt aattatcatg atcgatagcg gtttccaggc tggaatcgta
    19381 gtaatccata atctgttgag tattggcttc aagttgcggc caaatcttcg cggcgcgcgc
    19441 gagttgcccg ttgccttcca gcttatgaag aatgcgctca taaatactga aatgcccggc
    19501 agacaagtaa tcgaccaggc tctgacaaaa atcatcaagg gctttttcgt ttagcctcat
    19561 gtacgattct ttgccaggct taatgccaac cagattgtag taagccacga gcagatgctt
    19621 acgtacatgt agccagcgat caaccagttt gttacttcct ctgacgcgct ccgtcaggtt
    19681 atcgagctgg ttaagcatga ttgactccgc aagtttgtat tcaaaaactg ctcagtgaga
    19741 aatgtaaaaa ccatgttaaa catgccagtg atgcaaaggt agtgcaagag ctatggatcg
    19801 tataattgaa aaattagatc acggctggtg ggtcgtcagc catgaacaaa aattatggtt
    19861 gccgaaggga gaattgccat atggcgaagc ggcaaatttc gatcttgtgg gtcagcgcgc
    19921 actacaaatc ggcgaatggc agggggaacc tgtttggtta gtacaacagc agcggcgtca
    19981 cgatatgggg tcggtacgtc aggtcattga tctcgatgtt gggctgtttc aactggccgg
    20041 acgaggcgta caactggcgg agttttaccg atcgcataaa tactgtggtt actgcgggca
    20101 tgaaatgtat ccgagcaaaa ccgaatgggc gatgctgtgc agccattgcc gtgagcgtta
    20161 ctacccgcaa atcgccccct gcattattgt tgccatccgt cgcgatgatt cgatcctcct
    20221 cgcccagcat acccgccatc gtaacggtgt ccatacagta cttgccggat tcgtcgaagt
    20281 gggcgaaacc ctcgagcagg cagtcgcgcg ggaagtgatg gaagagagcg gaattaaagt
    20341 taaaaacttg cgttacgtga cttctcagcc gtggccgttt cctcagtctt taatgaccgc
    20401 gtttatggcg gaatatgaca gcggcgacat cgtgatcgac ccgaaagaat tgctcgaggc
    20461 gaactggtat cgctatgacg atttgccgtt actcccgccg cccggcaccg tagcgcgccg
    20521 tctgatagaa gatacggtgg cgatgtgtcg ggcagagtat gagtgatgat acactgaccg
    20581 cctgacgcac taaggaacag ccaaaatgac cgaacttaaa aacgatcgtt atctgcgggc
    20641 gctgctgcgc cagcccgttg atgtcactcc agtatggatg atgcgccagg cgggtcgcta
    20701 tctaccggaa tataaagcca cgcgcgccca ggcgggcgat tttatgtcgc tgtgcaaaaa
    20761 cgccgagctg gcgtgcgaag tgactttgca accgctgcgt cgctacccgc tggatgcggc
    20821 gatcctcttt tccgatatcc tcaccgtgcc ggacgcgatg gggttagggc tctattttga
    20881 agccggagaa ggtccgcgtt ttacctcgcc agtcacctgc aaagccgacg tcgataaact
    20941 gccaattccg gacccggaag atgagctggg ttacgtgatg aacgcggtgc gtaccattcg
    21001 tcgcgaactg aaaggcgaag tgccgctgat tggtttttcc ggcagcccgt ggacgctggc
    21061 gacctacatg gtggaaggcg gcagcagcaa agcgttcacc gtgatcaaaa aaatgatgta
    21121 tgccgatcca caggcgctgc acgcgctgct cgataaactg gcgaaaagtg tcactctgta
    21181 tctgaatgcg cagattaaag ccggtgctca ggcagtgatg attttcgaca cctggggcgg
    21241 cgtgcttacc gggcgcgatt atcaacagtt ctcgctctat tacatgcata aaattgttga
    21301 tggtttactg cgtgaaaacg acggtcgccg cgtgccagtc acgctgttta ccaaaggcgg
    21361 cggacagtgg ctggaagcga tggcagaaac cggttgcgat gcgctgggcc tcgactggac
    21421 aacggacatc gccgatgcgc gccgccgtgt gggcaataaa gtcgcgttgc agggtaatat
    21481 ggatccgtcg atgctgtacg cgccgccagc ccgcattgaa gaagaagtag cgactatact
    21541 tgcaggtttc ggtcacggcg aaggtcatgt ctttaacctt ggtcacggca ttcatcagga
    21601 tgtgccgcca gaacatgctg gcgtgtttgt ggaggcagtg catcgcttgt ctgaacaata
    21661 tcaccgctaa ggagtgatta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc
    21721 ttcttctgtg atccgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc
    21781 cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata
    21841 tccctcgctt gagctggtgg agtataaagt tgcccgcatc gccaccacca tgccttacat
    21901 tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg gcagcgtggg agatgctgtc
    21961 gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg
    22021 cgtcgccagc cattttggct tattggtgga tgtgccgacc attggcgtgg cgaaaaaacg
    22081 tctctgcggt aaattcgaac cgctctccag cgaaccgggc gcgctggccc cgctgatgga
    22141 taaaggcgag cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cgttgtttat
    22201 cgctaccggc catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa
    22261 aggctatcgt ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc
    22321 gttcgtgcgc tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat
    22381 ttgagaactc aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg
    22441 gcagcatgtc actttcatgg cttgcttatg cgaacgcatg taccccaatt acgccatgtt
    22501 ctgccagcaa accggttttg gtgatgggca gatttaccgt cgtattctcg atctcatctg
    22561 ggaaacgctg accgttaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga
    22621 agaagcgatt ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc
    22681 ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt ggtgaaacgc tcgaacacgc
    22741 ggtggaagtg agtaagacct ccatcacgac cgttgcgatg ctggaaatga ctcaggctgg
    22801 tcgcgaaatg agcgatgaag agcttaaaga aaacccagct gtagagcaag aatgggacat
    22861 tcagtgggaa atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa
    22921 aggccttagg gcagacctgc gtgaggcggg tgagagcaat attggtataa tttttcagca
    22981 ataagaccag aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc
    23041 cccccgtctg gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg
    23101 agagtgctat tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta
    23161 aggataactt atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc
    23221 caaaacccag gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa
    23281 agaaggcgat gctgtacaac tggttggttt cggtaccttc aaagtgaacc accgcgctga
    23341 gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc
    23401 ggcatttgtt tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa
    23461 cagttttaac gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct
    23521 ggcaggcgct ctgtttctca ctgcctgtag tcacaactct tcacttcctc cctttaccgc
    23581 cagtggattt gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa
    23641 tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc gataccacga cgcgagagta
    23701 tcgctggcag ggcgataacc tcacgctcat caatatcaat gtttacagca aaccgccggt
    23761 gaacattcgc gcgcgttttg acgatcgcgg tgatctgagc tttatgcaac gtgaatccga
    23821 tggggaaaag cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgatca
    23881 gatccgccag attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg
    23941 gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa accattaaac ctgatttaga
    24001 ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt
    24061 ggcatggctg gaagcgcccg aaggttcgca attactgtta gtggcaaact ctgatttctg
    24121 tcgctggcaa cccaacgaga aaacgttctg atttaccagt ggcccatacc catatgaccg
    24181 ccaccaccgc agccgccgta gcccatgccc attccggctc cgcgcggaat acccgcttca
    24241 gccatcgcga tatcccgttt cacccgtaac tcatctaacg actgacgcaa attctccatc
    24301 tctttggcga ccgcgttaat tttgctgcta tccggtgggt tcgcggctaa cagggcattg
    24361 tattcataac gcttcgtcac cagttgctgt tgcagtgcgc tgctttgagc gtaaaagtca
    24421 ttatggattt tctgccacgc tgtctgttgt tcgctggtca aaggcgcggc attttgctgc
    24481 cacataccgt gtccgccgtg agcaaatgca gatgtcgatc ccatcgccat tgctgaaagc
    24541 gccatcatta ccagggcaat tttcgtgttc cgtttcatgg ttaatcctcc agtggttgtc
    24601 ttacttcggg tattgcatct tccgtgccaa cgatgaaacg ctgatatgac gggtaatctg
    24661 gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta gcgagtcatt tttactcatt
    24721 gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg ataaaagatg gcatgatttc
    24781 tgctgtcaga aagggatgag caggcaaaga agaagatgcg ttttatgcaa cgttctaaag
    24841 actccttagc taaatggtta agcgcgatcc tccccgtggt cattgttggg ctggtagggt
    24901 tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac tgccgccgcc agacaaacgc
    24961 tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc cggctcgcgc gtcggcatgg
    25021 ggatgcgcat gcatcatgcg cagcagcagg ccttactgga agaaatggcc gggcagcctg
    25081 gagtacgttg gtttgcggtc acggatgaac aaggaacaat cgtgatgcat agcaactccg
    25141 gcatggtggg aaaacagctt tattccccgc aggaaatgca gcagttacat ccgggagatg
    25201 aagaagcgtg gcggcggatc gatagcgcag acggcgagcc tgttctggaa atttatcgcc
    25261 agtttcaacc gatgtttgct gctggaatgc accggatgcg ccatatgcag cagtatgccg
    25321 cgacaccaca agcaattttc atcgctttcg acgccagtaa tattgtgagt gccgaagatc
    25381 gtgagcagag aaacaccctg attatcctct tcgccctggc gacggtcttg ctggcaagcg
    25441 tattgtcatt cttctggtat cgccgctatc tgcgctcgcg ccagcttcta caagatgaaa
    25501 tgaagcgcaa agagaagcta gtggcgctgg ggcatcttgc ggcaggcgtt gcccacgaaa
    25561 tccgtaaccc actttcctcg attaaaggac tggcgaaata ctttgccgag cgcgcgcctg
    25621 cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa agaggccgac cgtttaaacc
    25681 gcgtggtaag cgagctgctg gaactggtta agccaacgca tctggctttg caggcggtgg
    25741 atctcaacac gctgattaac cactcattac agctggtagg ccaggatgca aacagccggg
    25801 agatccagtt acgctttacc gccaacgaca cattaccgga aattcaggcc gatccggaca
    25861 ggctgactca ggtcctgttg aatctctatc tcaatgctat tcaggcgatt ggtcagcatg
    25921 gcgtgattag cgtgacggtc agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca
    25981 gcggtaaggg aattgcggca gatcagcttg aagccatctt cactccgtac ttcaccacca
    26041 aagccgaagg caccggattg gggctggcgg tcgtgcataa tattgttgaa caacacggtg
    26101 gtacaattca ggtcgcaagc caggagggaa aaggctcaac gttcaccctc tggcttccgg
    26161 tcaatattac gcgtaaggac ccacaaggat gacgcacgat aatatcgata ttctggtggt
    26221 ggatgatgac attagccact gcactatttt gcaggcttta ctgcgcggct ggggctataa
    26281 cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag gtgcgggaac aggtttttga
    26341 tcttgtgctt tgcgatgtgc gaatggcgga gatggacggc atcgccacgc tgaaagagat
    26401 caaagcgtta aacccggcaa ttccggtgct gattatgact gcgtactcca gcgtcgagac
    26461 ggcggtagag gcactgaaaa ctggggcgct ggattatctc atcaagccgc tggatttcga
    26521 caccctgcag gcgacgctgg aaaaggcgct cgcgcatacg cacagtattg atgctgaaac
    26581 acctgcggtg actgccagcc agttcggtat ggtcggtaaa agctcggcga tgcaacacct
    26641 gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg gtactgatcc acggcgattc
    26701 cggcaccggt aaagagctgg tcgccagggc gattcacgcc agtagcgcac gtagcgaaaa
    26761 accgctggta acgctcaact gtgcggcact caacgaatcc ttgctggaat ctgaattgtt
    26821 cggtcacgaa aaaggggcgt ttactggagc cgataaacgc cgggaagggc gctttgttga
    26881 ggcggacggc ggcacgctgt ttctcgatga aattggcgat atctcgccga tgatgcaggt
    26941 gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt gtcggtagca accagactat
    27001 ctcggttgat gtccggctga ttgcggcgac ccatcgcgat cttgccgcag aggtgaatgc
    27061 cgggcatttt cgccaggatc tctactatcg cctgaatgtg gtggcgattg aagtaccatc
    27121 gctgcggcaa cggcgggaag atatccctct gctggctggc cattttcttc agcgctttgc
    27181 cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag gcgatggatt tgctgatcca
    27241 ctacgactgg ccgggaaata ttcgtgaact ggaaaacgcg gtggaacggg cggtggtgct
    27301 gctgaccggg gaatatattt ccgaacgcga gctgccgctg gcgattgccg gtacgccgat
    27361 cccgctgggg caaagtcagg atattcagcc gctggtagaa gtcgaaaaag aagtgattct
    27421 ggcggcactg gagaaaacgg gcggcaacaa aaccgaggcc gcccgtcagt tagggatcac
    27481 gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc tcgcgttcga tagcgcgcca
    27541 gccaatatct ttccggcaga agcagtcgtc ccagtgaata tcggtcatta aggcataagc
    27601 gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc
    27661 gctggtcact acctgctcgt tatccgccag tttcgtgccc gcgtggaaca ctttgccgtc
    27721 agccacttct tccagcggca ggccgtggat cacatcgccg gtgcggtaat cgcccggata
    27781 tccacccgca gccatcacca cgccgagaga agcgcgttca tcccactcgg atgttttctc
    27841 gtccagcttc ccttcacagg ctgccaggca gagctcaacc agatcggact tcatgcgcag
    27901 cataatcggc tgggtttccg gatcgccaaa gcggcagtta aattcgataa ccttcggatt
    27961 gccctgtttg tcgatcatca ggcccgcgta gagaaaaccg gtgtaggtgt tgccttccgc
    28021 cgccatgcct ttcacggttg gccagatgat acgttccatg gtgcgctgat gaacttcatc
    28081 ggttactacc ggcgcggggg agtaagcgcc catcccgccc gtgtttggtc cggtatcttt
    28141 atcgcctacg cgtttgtgat cctggctggt agccatcggc aacacatgct cgccgtccac
    28201 catcacgata aagctcgctt cttcgccatc gaggaactct tcgataacga tgcgatgacc
    28261 cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca gccgcttccg cttcttccag
    28321 cgtcatcgcc acgataacgc ctttcccggc agccagaccg tccgctttaa tgacgattgg
    28381 cgcgcctttc tcacgcagat acgccagcgc aggttctacc tcggtgaagt tctggtattc
    28441 cgccgtaggg atcttatggc gggccaggaa atctttggta aacgcttttg agccttccag
    28501 ttgggccgca cctgcggttg ggccgaagat tttcagcccg gcggcgcgga aggtatcgac
    28561 cacgcctttc accagcggcg cttccgggcc gacgatggtc agatcaatct tttcgttttg
    28621 tgcgaaatcc aacagcgccg ggatatcggt cacgccaata gcaacgtttt gcagcgcggg
    28681 ttccagcgca gtgcctgcat tacccggagc aacaaaaaca gtctcaacca gcggcgactg
    28741 ggccgctttc caggccagcg cgtgctcgcg cccgccgtta ccaatcacta atactttcat
    28801 ctattgctcc attaatggcg gaagtggcgc atgtcggtga agagcatcgc aataccgtgc
    28861 tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac cgccaggctg gattacgcag
    28921 gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga acgggaagaa cgcgtcagaa
    28981 gccatcgagg aacctttcac ttccaggcct tcatcagcag ctttaatacc ggcgattttc
    29041 gcggagtaca cgcggctcat ctggcccgcg ccaataccga tggtcatatt gtttttggca
    29101 tagacgatag cattggattt cacgaacttc gccactttcc agcagaacag cgcatcgcgc
    29161 agttcctgtt cggtcggctg acgttgggtg acgacgcgca gttcttccgc accgaccatc
    29221 cccaggtcac gatcctgaac cagcagaccg ccgttcacgc gtttgaaatc aagacccgga
    29281 acacgctcgc cccactgacc gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt
    29341 ttcagggctt cttcgctggc ggaaggcgca ataatcactt cgacaaactg acgagaaatg
    29401 atggcctgtg cggtttccgc atccagctcg cggttaaagg caatgatgcc gccgaatgcg
    29461 gaggttgggt cggttttgta cgcgcgatcg taagcatcaa gaatggaatt gccgatagct
    29521 acaccgcaag ggttggcgtg cttcacaatc acacatgccg gctcggcgaa ctctttcacg
    29581 cactccagcg ccgcatcggt atcggcgatg ttgttataag agagggcttt accctgaacc
    29641 tgggttgcgg tagcaacgga ggcttctttc acattctctt ctatatagaa ggcagcctgc
    29701 tggtggctgt tctcgccgta acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg
    29761 cgtgggaagc gaccagcggc ttctttgctt tcaccgtggt acgccggaac catgctgccg
    29821 aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg
    29881 tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat ccatctcttt aataatggcg
    29941 tcgtagtcgc tgctcttcac cacgattgcg acatctttat ggttcttagc ggcggagcgc
    30001 accatggtcg ggccgccgat atcgatgttc tcaaccgcat cttccagcga gcaaccttca
    30061 cgggccacgg tctgggcgaa cggatacagg ttaacaacca ccatatcgat aggctggatc
    30121 tgatgttctt ccataatggc atcgtcctgg ccgcgacggc ccagaatgcc accatgtact
    30181 ttcggatgca gggtcttcac gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa
    30241 acttcggtta ccggcagacc tttttctgct aacagacggg cagtgccccc tgtagacagc
    30301 agctccacac cgcgtgcgga aagtgcctgg gcgaattcga cgataccggc tttgtcagaa
    30361 acactgagca gagcgcggcg gactggacga cgttgttgca tggtaaatcc cctggatttg
    30421 actattacag agagcgttag ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg
    30481 ggcatcctta tttttcgccc gcattgtaac gaaaacgttt gcgcaacgct cgcgaatttt
    30541 tctctttcaa tggtgatcac aattttgaca gtggttaccg tgggaaaaat acagaaatta
    30601 cattgatgat tgtggataac tctgtgcgta aaaaggtata aagcggggtt ttgctgggga
    30661 atgcagcagt cagtcatttt tctgcaattt ttctattgcg gcctgcggag aactccctat
    30721 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    30781 aaga
//
LOCUS       DAEVZA010000046        30689 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEVZA010000046 DAEVZA010000000
VERSION     DAEVZA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.046"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            9..408
                     /gene="tnpA"
                     /locus_tag="LAF92_002163"
                     /pseudo
     CDS             9..408
                     /gene="tnpA"
                     /locus_tag="LAF92_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1362..2159)
                     /locus_tag="LAF92_002164"
     CDS             complement(1362..2159)
                     /locus_tag="LAF92_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9791167.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLTSRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(2169..2720)
                     /gene="ybcL"
                     /locus_tag="LAF92_002165"
     CDS             complement(2169..2720)
                     /gene="ybcL"
                     /locus_tag="LAF92_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBI9791168.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(2889..3221)
                     /gene="emrE"
                     /locus_tag="LAF92_002166"
     CDS             complement(2889..3221)
                     /gene="emrE"
                     /locus_tag="LAF92_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBI9791169.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(3555..3869)
                     /gene="fliE"
                     /locus_tag="LAF92_002167"
     CDS             complement(3555..3869)
                     /gene="fliE"
                     /locus_tag="LAF92_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBI9791170.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4084..5742
                     /gene="fliF"
                     /locus_tag="LAF92_002168"
     CDS             4084..5742
                     /gene="fliF"
                     /locus_tag="LAF92_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBI9791171.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQRSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            5735..6730
                     /gene="fliG"
                     /locus_tag="LAF92_002169"
     CDS             5735..6730
                     /gene="fliG"
                     /locus_tag="LAF92_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBI9791172.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            6723..7409
                     /gene="fliH"
                     /locus_tag="LAF92_002170"
     CDS             6723..7409
                     /gene="fliH"
                     /locus_tag="LAF92_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBI9791173.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7409..8782
                     /gene="fliI"
                     /locus_tag="LAF92_002171"
     CDS             7409..8782
                     /gene="fliI"
                     /locus_tag="LAF92_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBI9791174.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGTETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            8801..9244
                     /gene="fliJ"
                     /locus_tag="LAF92_002172"
     CDS             8801..9244
                     /gene="fliJ"
                     /locus_tag="LAF92_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBI9791175.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            9241..10368
                     /gene="fliK"
                     /locus_tag="LAF92_002173"
     CDS             9241..10368
                     /gene="fliK"
                     /locus_tag="LAF92_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBI9791176.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPHVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQHSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            10473..10937
                     /gene="fliL"
                     /locus_tag="LAF92_002174"
     CDS             10473..10937
                     /gene="fliL"
                     /locus_tag="LAF92_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBI9791177.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            10942..11946
                     /gene="fliM"
                     /locus_tag="LAF92_002175"
     CDS             10942..11946
                     /gene="fliM"
                     /locus_tag="LAF92_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBI9791178.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11943..12356
                     /gene="fliN"
                     /locus_tag="LAF92_002176"
     CDS             11943..12356
                     /gene="fliN"
                     /locus_tag="LAF92_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBI9791179.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12359..12724
                     /gene="fliO"
                     /locus_tag="LAF92_002177"
     CDS             12359..12724
                     /gene="fliO"
                     /locus_tag="LAF92_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBI9791180.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            12724..13461
                     /gene="fliP"
                     /locus_tag="LAF92_002178"
     CDS             12724..13461
                     /gene="fliP"
                     /locus_tag="LAF92_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBI9791181.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13471..13740
                     /gene="fliQ"
                     /locus_tag="LAF92_002179"
     CDS             13471..13740
                     /gene="fliQ"
                     /locus_tag="LAF92_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBI9791182.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            13749..14534
                     /gene="fliR"
                     /locus_tag="LAF92_002180"
     CDS             13749..14534
                     /gene="fliR"
                     /locus_tag="LAF92_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBI9791183.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            14824..15447
                     /gene="rcsA"
                     /locus_tag="LAF92_002181"
     CDS             14824..15447
                     /gene="rcsA"
                     /locus_tag="LAF92_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBI9791184.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15491..15679)
                     /gene="dsrB"
                     /locus_tag="LAF92_002182"
     CDS             complement(15491..15679)
                     /gene="dsrB"
                     /locus_tag="LAF92_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBI9791185.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            15842..16069
                     /gene="yodD"
                     /locus_tag="LAF92_002183"
     CDS             15842..16069
                     /gene="yodD"
                     /locus_tag="LAF92_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBI9791186.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            16367..17182
                     /locus_tag="LAF92_002184"
     CDS             16367..17182
                     /locus_tag="LAF92_002184"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HBI9791187.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGGHLHDEDPAHVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(17179..18873)
                     /gene="yedQ"
                     /locus_tag="LAF92_002185"
                     /pseudo
     CDS             complement(17179..18873)
                     /gene="yedQ"
                     /locus_tag="LAF92_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(19044..19226)
                     /locus_tag="LAF92_002186"
     CDS             complement(19044..19226)
                     /locus_tag="LAF92_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBI9791188.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(19305..20222)
                     /locus_tag="LAF92_002187"
     CDS             complement(19305..20222)
                     /locus_tag="LAF92_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBI9791189.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            20395..21315
                     /gene="yedA"
                     /locus_tag="LAF92_002188"
     CDS             20395..21315
                     /gene="yedA"
                     /locus_tag="LAF92_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBI9791190.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21304..21774)
                     /gene="vsr"
                     /locus_tag="LAF92_002189"
     CDS             complement(21304..21774)
                     /gene="vsr"
                     /locus_tag="LAF92_002189"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBI9791191.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(21755..23173)
                     /gene="dcm"
                     /locus_tag="LAF92_002190"
     CDS             complement(21755..23173)
                     /gene="dcm"
                     /locus_tag="LAF92_002190"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBI9791192.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23240..23935)
                     /locus_tag="LAF92_002191"
     CDS             complement(23240..23935)
                     /locus_tag="LAF92_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBI9791193.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(23975..24340)
                     /locus_tag="LAF92_002192"
     CDS             complement(23975..24340)
                     /locus_tag="LAF92_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI9791194.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKPALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            24906..25979
                     /locus_tag="LAF92_002193"
     CDS             24906..25979
                     /locus_tag="LAF92_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063070036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBI9791195.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGECIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNLMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRGVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
     gene            26572..27423
                     /gene="hchA"
                     /locus_tag="LAF92_002194"
     CDS             26572..27423
                     /gene="hchA"
                     /locus_tag="LAF92_002194"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBI9791196.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(27531..28889)
                     /gene="hprS"
                     /locus_tag="LAF92_002195"
     CDS             complement(27531..28889)
                     /gene="hprS"
                     /locus_tag="LAF92_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBI9791197.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNYSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(28889..29560)
                     /gene="hprR"
                     /locus_tag="LAF92_002196"
     CDS             complement(28889..29560)
                     /gene="hprR"
                     /locus_tag="LAF92_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBI9791198.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            29693..30106
                     /gene="hiuH"
                     /locus_tag="LAF92_002197"
     CDS             29693..30106
                     /gene="hiuH"
                     /locus_tag="LAF92_002197"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBI9791199.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            30215..>30689
                     /locus_tag="LAF92_002198"
     CDS             30215..>30689
                     /locus_tag="LAF92_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBI9791200.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAATLSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIG"
BASE COUNT         7921 a         7732 c         7313 g         7723 t
ORIGIN      
        1 tttggtctat gaataaagaa accgatatcc gacggggaag gcattgtgtt ttcctgatgc
       61 atgttcacct ggtctttgtc accagatacc gacgccagat ttttgaccat gacgcgacag
      121 aaaaactacg cacttacttt tcaaatgtat gtgctgattt tgaagctgaa ctggttgaaa
      181 tggatggcga accagatcac gtccatttgt taattaatta tcctaccaaa ctggcgatat
      241 ccagtctggt aaacagcctc aaaggcgtat cgggtaggtt actgcgacga gatcgaccag
      301 atattgcagt caggtattac tacaaaggcg ttttgtggag tgctggctat tttgccagta
      361 gctgcggagg tgcgccaata cattgaacaa cagcaaacac ctggttaggt ggaaaaccgc
      421 gccttatatc cctgccctga aggacggggt tttacggcgc accggataag cctggagatt
      481 atctacacca gcatggacat cattgtacta tgccccgtac atgccccatc acatcctcaa
      541 catcaaagta ttagagcctt gcaaaccatt atgcgtctca gtttgtctca tctatcttac
      601 gcaaaaaaaa tagaactaat atcaagcggc gctatattgc gtattaaata aaaccacctc
      661 aattacatta ataaatctaa acaaacatca tgcaaataat ttccatggtt atttctaggc
      721 tatttaaatt aaataaataa attatgagtc aatcataaat aacaaccttt aatataattt
      781 gactaccaaa tttatattcg ttcacaaaat agtcatgcat tgtgtgcaat agaaacagtt
      841 cagataaaga tagggatcta cttcacaatt taaatgagaa actaaaactt acatcttgaa
      901 ataacctcat tgattggatg aatatttgta gtgcagtgac attattttta aataatagtt
      961 caaataaaag ggctcacgat gaaaaaaatt aacagtggca atttctgctg tagctgcatc
     1021 ttaacgtata aactagattt aataacaacg aacaagattt aataacaacg atatccgtac
     1081 ctatccggat atctaatgtc ataagcatcc ttttaattat cccctcaacc tcttgtaact
     1141 ttcccactct tgaactccac atagtccaaa cgccggatgc aaaaaaatca cacgtgcatt
     1201 tctgaaattg tgatggagga cgatagtatc aattatatgt gcttataaac gtccccgatt
     1261 agcgcagaag tcaccggatt gttcaggctc caataggttg agtatgccgt atttattttc
     1321 caaaataaaa atcgatgctg ttgctccttc ataaaatact tttactctct ggacgctttt
     1381 cttagctctc ttggtgttat cttaaaacga ttatggaacc tttcagtaaa acgagaagga
     1441 cacttgtaac cattttccct ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca
     1501 gacaacgcat tatttaacct cacgtcgtcc aatatacttt gaaaacttac tccctcgctt
     1561 gccagacggc gatgcaatgt ggaaacagaa atgtagagat atctcgctac cttgtttgct
     1621 gtccattttg ttccaggttc ggatagcatt aggttataac aacgacttgt taatgattgt
     1681 ttactatctg ataaaagtaa acaattaaca tgatgtgctc ctaatgaaag tagaacgccc
     1741 attgctaagt gttcctgaat ttgcgttgag aagcctcggg aaatagatgt ttttagttgc
     1801 tcccaacaat atattaactc gggattctga ggtaagcaga aacctgtcgt gttacgaatt
     1861 tgatcggtta tcgcataaag attttggaac ttttcaatta gatcaatggg ataataaagc
     1921 atttctgcaa gataaagacc tgcttctggg taattcgcaa tataaaattc atatccacag
     1981 ggaaataata ttatctgatt attatcaaca gttagactat gcgtctccca attgataact
     2041 ttttttccct gacggatacg acataaagct ggcataagag gcttaaccct gtgaatctca
     2101 tgatgtttat gcatccgtat ttcttcaatc tttaggttag tcctatcttt tgccagcata
     2161 ctcttatcct actttacctc ataaacgggt attatctcag cggttgcaat tttattggca
     2221 ttaagcatat aaccaactaa cgctccgctg gaattagaat ccacaggaat cttatcagtt
     2281 tttagagccc atattttaaa ctggtaatga tggggtttat ctcctttggg aggacatgct
     2341 ccaccaaatc ccgcatagcc aaaatcattt cggccttgga cagcaccagt cggaagtttt
     2401 gtaccatcac gtcttcctgc atcagcgggc aaatatgtta ccgttgctgg aatattagca
     2461 acagtccagt gccaccatcc gctgcctgta ggcgcatctg gatcatacac agttacggca
     2521 aagcttttgg tgccttctgg agcaccagac caggttaatg agggcgatgt attacccccc
     2581 tcacacccaa atccagaaaa tacatgagac gttgtaagtt gctctcctgt ttttatttca
     2641 ttactagtga cctgaaatgc tgcggcctgc gcagaaaatg ttatgaatgc caatacactt
     2701 gaaacgataa gttttttcat aaaaacctct ttgttgtgac ctatcgttat tttatttgat
     2761 gtgcattgat ctcattatgc aaaaaggcgc aatgatcgcg caaatgcaat cataattata
     2821 aagttgcccc gaacattagc cataatatgc acaaatcaga ttcctgttat ctcaaggatg
     2881 attatttttt aatgcggtgc gcttcgtgac aacaagttaa tgactaacac accggcacaa
     2941 atcaacatca tgcctatgat ggctggcaag tccagtcgtt ggccaaaaaa tccccaggaa
     3001 agcaaactaa tcagaacgat accgactcct gaccagatag cataagcaat ccctgtaggt
     3061 atataagcca gtgtctgagc taataaccaa aatgatgcac aatagcaaat aattgtgcca
     3121 acagagggcc ataaccgtgt aaacccctct gaaaacttca ttaaggttgt accaatgacc
     3181 tctgcaagta ttgcgccacc aagataaata taagggttca tagcatattc tttcctgttc
     3241 atactggaga gaattgtact acagtttgaa cacaactcac cgttttcatc ctggtgtatg
     3301 tgtatccagg gggctctggc gtggcaagag ataaaggcag gaacaataat gatcagaagg
     3361 aaccccaggc taacaaaaga gttcactctt cctatgagca aagcgaagac aaaacatgtt
     3421 atcgcgttta cggatcgtgg atggcagaaa actttcagga tcaggtatcc attttaaacc
     3481 agaaattgag tgagtttgcc ccatccatgc cccaagcggt gagattggaa gtaattaaca
     3541 acaggttaca aaacctacac ctgcatggac atcacttcct gatacgccgc caccagctta
     3601 ttacgcacct gaatccccat ttgcatagaa actgaggctt tttgcatatc ggtcatcaca
     3661 tcgtttaacg ccacgccggg ttcaccgagg gtgaattttt ctgcctgcgt gcgggcagct
     3721 gtttgtgtat cgctaatgcg atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc
     3781 ggttgcggca gtgaatcctg cgcacgcgca ctcatcgccg tagcctgtaa ctggctgata
     3841 accccttcaa tcccctgtat cgctgacatt ttcgtctcct ggataatttc tggtagcaaa
     3901 gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac gggtttattg
     3961 gcggatagaa aaaaacgaaa gcacaaataa ttgcagcgtc aatttttcga gtttgctgac
     4021 ccgggagtga gtcttgttcc actttgccaa taacgccgtc cataatcagc cacgaggtgc
     4081 gcgatgaatg cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg
     4141 aatccgaaaa ttccattgat tgttgccggt tccgcggcag tggcggtcat ggtcgcactg
     4201 atcctgtggg cgaaagcccc cgactaccgc acattattca gcaatctttc cgatcaggat
     4261 ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc cttaccgctt cagcgaagcc
     4321 agcggcgcta ttgaagttcc ggcagataaa gttcacgaac tgcgtctgcg cctggcacaa
     4381 caaggtttgc ccaaaggcgg cgcggtcggt ttcgaactac tcgatcagga aaagtttggt
     4441 atcagccagt tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt
     4501 accatcgcaa ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg
     4561 tctttattcg tccgtgaaca aagatcccct tctgcatcgg tgacggtaaa tctgttaccc
     4621 ggccgcgcac tcgatgaagg gcaaattagc gccattgtgc atctggtttc cagcgccgtt
     4681 gctggtctgc cgccgggaaa cgtcacgctg gtggatcagg gcggacatct gttaacccag
     4741 tccaatacca gcgggcgcga tcttaatgat gctcagttga aatatgccag cgatgtcgaa
     4801 ggccgtattc agcggcgtat tgaagcaatc ctgtcgccta ttgttggtaa cggtaatatt
     4861 cacgcccagg tcacggcgca gctggacttc gccagtaaag aacaaacgga agaacagtat
     4921 cgccctaacg gtgatgaatc tcatgcggcg cttcgttcac gccagcttaa tgagagcgag
     4981 caaagcggtt ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca accggcacct
     5041 gcgaataacg cgccaatcag cacgcctccg gcaaatcaaa ataaccgcca gcagcaggcg
     5101 agcaccacca gcaatagcgg gccgcgtagc acacagcgga atgaaaccag taactacgaa
     5161 gtcgatcgca ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc
     5221 gcggtagtgg tgaattacaa aaccttgcca gacggtaaac cattgcctct cagcaacgaa
     5281 cagatgaagc aaattgaaga tctgacccgc gaggcgatgg gcttttctga aaaacgcggt
     5341 gactcgctca atgtcgttaa ctcgccgttc aatagcagtg acgaaagcgg cggagaactg
     5401 ccgttctggc aacagcaagc gtttatcgat cagttgctgg ctgccggtcg ctggttgctg
     5461 gtactgctgg tggcgtggct gctgtggcgg aaagcggtac gtccgcagct aacacgtcgc
     5521 gctgaggcga tgaaagctgt acagcaacag gcgcaggccc gcgaggaagt ggaagatgcg
     5581 gtggaagtcc gcctgagcaa agacgaacaa ctacaacaac ggcgcgctaa ccaacgtctg
     5641 ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg
     5701 gcgctggtca ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa
     5761 aagcgtcatc ctgctgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct
     5821 ctcccagcgt gaagtacaaa ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa
     5881 caagcagcta accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgccgcact
     5941 gaatatcaac gccaacgatt atctgcgctc ggtattggtc aaagctctgg gtgaagaacg
     6001 tgccgccagc ctgctggaag atattctcga aactcgcgat accgccagcg gtattgaaac
     6061 gctcaacttt atggagccac agagcgccgc cgatctgatt cgcgatgagc atccgcaaat
     6121 tatcgccacc attctggtgc atctgaagcg cgcccaagcc gccgatattc tggcgttgtt
     6181 cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc
     6241 agccgcgctg gcggagctga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa
     6301 gcgcagcaaa atgggcggcg tgagaacggc agccgaaatt atcaacctga tgaaaactca
     6361 gcaggaagaa gccgttatta ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat
     6421 catcgacgag atgttcctgt tcgagaatct ggtggatgtc gacgatcgca gcattcagcg
     6481 tctgttgcag gaagtggatt ccgaatcgct gttgatcgcg ctgaaaggag ccgagcagcc
     6541 actgcgcgag aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga
     6601 tctcgccaac cgtggtccgg tgcgtctgtc gcaggtggaa aacgaacaga aagcgattct
     6661 gctgattgtg cgccgccttg ccgaaactgg cgagatggta attggcagcg gcgaggatac
     6721 ctatgtctga taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccacagg
     6781 cagagtttgt gcccatagtc gagccggaag aaaccatcat tgaagaggct gaacccagcc
     6841 ttgagcagca actggcgcaa ctgcaaatgc aggcccatga gcaaggttat caggcgggta
     6901 ttgccgaagg tcgccagcaa ggtcataagc agggctatca ggaaggactg gcccaggggc
     6961 tggagcaagg tctggcagag gcgaagtctc aacaagcgcc aattcatgcc cggatgcagc
     7021 aactggtcag cgaatttcaa actacccttg atgcacttga tagtgtgata gcgtcgcgcc
     7081 tgatgcagat ggcgctagag gcggcacgtc aggtcatcgg tcagacgcca acggtggata
     7141 actcggcact gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggta
     7201 aaccacagct gcgcgtgcac ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcta
     7261 ccttaagttt gcatggctgg cgcttgcggg gcgatcccac cctccatcct ggcggctgta
     7321 aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct
     7381 gccgtctggc agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac
     7441 gctggataac tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct atgggcgatt
     7501 aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac
     7561 ctgtgtcatt gaacgccaga acggcactga aacgcacgaa gtagaaagcg aagtcgttgg
     7621 ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtcgaaggtg tcctgcccgg
     7681 cgcgcgggtt tatgccaaaa acatttcggc tgaaggactg caaagcggca agcagttgcc
     7741 gctcggtccg gcgttattag gtcgcgttct ggacggcagc ggtaagccgc tcgatggcct
     7801 gccctccccc gatacgacgg aaaccggtgc gctgattacc ccgccattta acccgttgca
     7861 acgtacaccg attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac
     7921 cgtcgggcgc gggcagcgta tggggctgtt tgccgggtct ggcgttggta aaagtgtgct
     7981 gctggggatg atggcccgtt acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga
     8041 acgtgggcgc gaagtaaaag attttattga gaatatcctc ggtgccgagg gacgtgcacg
     8101 ctccgtggtg attgccgctc cggcggatgt ctctccgctt ctgcgaatgc aaggtgccgc
     8161 ctatgccacg cgaattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat
     8221 ggactccctc acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc
     8281 acctgccact aaaggttatc caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg
     8341 tgccggtaat ggcattagcg gcggcggatc gattactgca ttttataccg tgctcactga
     8401 aggcgatgac cagcaggacc ccattgccga ctctgcgcgg gcaatcctcg acggtcacat
     8461 tgtgctgtct cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc
     8521 gatcagccgc gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt
     8581 caaacagctg ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc
     8641 caaaggcagc gatccgatgc tcgacaaagc catcgccctg tggccgcagc tggagggcta
     8701 tttgcaacaa ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg
     8761 tattttcccg acagtgtcat aacccaggag ataacggcag atggcagaac atggcgcgct
     8821 ggcgaccctg aaagatctgg cagaaaaaga ggtagaggat gccgcgcgcc tgctgggtga
     8881 aatgcgtcgc gggtgtcagc aggcggaaga acagctcaaa atgctgatcg attatcagaa
     8941 tgaatatcgc aataacctca acagcgatat gagtgccggg atgaccagca accgctggat
     9001 caactatcag cagtttatcc agacgctgga aaaagccatt actcagcatc gccagcaact
     9061 taatcagtgg acgcagaaag ttgacattgc cctgaacagc tggcgagaaa agaaacaacg
     9121 tttgcaggcc tggcagacac tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa
     9181 ccgcctcgat cagaaaaaga tggatgagtt cgcccagcgc gccgccatga ggaaacctga
     9241 atgattcgct tagcgccctt aattaccgcc gacgttgaca ccaccacatt gcctggcggc
     9301 aaagccagcg atgctgcaca agattttctc gcgttgttga gcgaagcatt agcaggcgag
     9361 acaactaccg acaaagcggc cccccagttg ctggtggcaa cagataagcc cacgacaaaa
     9421 ggcgagccgc tggtcagcga gattcttgcc gatgcgcaac aagcggattt actgatccct
     9481 gtggatgaaa caccgcatgt catcaacgac gaacaatcca catcaacacc attaaccacc
     9541 gctcaaacga tgacgttggc tgcggtggct ggcaacaata cggcaaaaga cgaaaaagcg
     9601 gatgatctga atgaagacgt caccgcaagc ctgagcgccc tttttgcgat gttgccgggt
     9661 tttgacaata cgcccaaagt gactgatgtg ccgtcaaccg tgttaccggc agagaaacca
     9721 acgctcttca caaaactgac ttctgcgcaa ctcacaacag cacagcctga tgatgccccc
     9781 ggcacgccag ctcagccatt aacaccgctg gtagcagaag cccagagtaa agcggaagtc
     9841 atcagcacac cttcaccggt gaccgctgcc gccagcccgc taatcactcc acaccagaca
     9901 cagccactgc ccaccgtcgc cgcgcctgtg ttgagtgcac cgctgggttc tcacgaatgg
     9961 caacattcat taagccagca tatttcgctg ttcacccgcc aggggcaaca aagtgcagag
    10021 ttgcgtctgc acccgcagga tttaggtgaa gtgcaaatct ccctcaaagt ggatgataac
    10081 caggcgcaaa tccagatggt ttcaccgcat caacacgtac gcgccgccct ggaagcagcg
    10141 cttccggtac tgcgaacgca gctggccgaa agtggcattc agttagggca aagcaacatc
    10201 agtggcgaaa gctttagtgg tcagcagcag gccgcttccc agcaacagca aagccaacgc
    10261 acagcaaacc atgaacctct ggcgggggaa gacgacgata cgcttccggt tcccgtctct
    10321 ttacaagggc gcgtaacagg caacagcggc gttgatattt tcgcctaacg tcagaggtag
    10381 caccgtaatc cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat aattagccga
    10441 taagcagtag cgacacagga agaccgcaac acatgactga ttacgcgata agcaagaaaa
    10501 gcaagcgatc gctttggatc ccgattctgg tattcattac cctcgcggcc tgtgccagcg
    10561 caggttacag ctactggcat tcgcatcagg ttgccgctga cgacaaagcg cagcaacgcg
    10621 tcgtgccctc cccggtcttc tatgcgctgg atacctttac ggtcaatctg ggcgatgcgg
    10681 atcgcgtgct gtatatcggc ataaccctgc gcctgaaaga tgaagctaca cgctcgcggc
    10741 tgagtgagta tttgccggaa gtccgtagcc gtttgctgtt actgttttcg cgtcaggatg
    10801 ctgccgtact ggcgacagaa gaaggcaaga aaaacctgat tgccgagatt aaaaccacgc
    10861 tttccacccc gcttgttgcc gggcaaccga aacaggatgt caccgacgtg ctgtataccg
    10921 cttttattct gcgataacga catgggcgat agtattcttt ctcaagctga aattgatgcg
    10981 ctgttgaatg gtgacagcga agtcaaagac gaaccgacag ccagtgttag cggcgaaagt
    11041 gacattcgtc cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg
    11101 ctggaaatca ttaatgagcg ctttgcccgc cattttcgta tggggctgtt caacctgctg
    11161 cgtcgtagcc cggatataac cgtcggggcc atccgcattc agccgtacca tgaatttgcc
    11221 cgcaacctgc cggtgccgac caacctgaac cttatccatc tgaaaccgct gcgcggcaca
    11281 gggctggtgg tgttttcacc gagtctggtg tttatcgccg tggataacct gtttggcggc
    11341 gatggacgct tcccgaccaa agtggaaggt cgcgagttta cccataccga acagcgcgtc
    11401 atcaaccgca tgttaaagct ggcgcttgaa ggctatagcg acgcctggaa ggcgattaat
    11461 ccgctggaag ttgagtacgt gcgttcggaa atgcaggtga aatttaccaa tatcaccacc
    11521 tcgccgaacg acattgtggt taacacgccg ttccatgtgg agattggcaa cctgaccggc
    11581 gaatttaata tctgtctgcc attcagcatg attgagccgc tacgggaatt gttggttaac
    11641 ccgccgctgg aaaactcgcg taatgaagat cagaactggc gcgataacct ggtgcgccag
    11701 gtgcagcatt cacagctgga gttggtcgcc aactttgccg atatctcgct acgcctgtcg
    11761 cagattttaa aactgaaacc cggcgacgtc ctgccgatag aaaaacccga tcgcatcatc
    11821 gctcatgttg acggcgttcc ggtactgacc agtcagtacg gcaccctcaa cggtcagtat
    11881 gcgttacgga tagaacattt gattaacccg attttaaatt ctctgaacga ggaacagccc
    11941 aaatgagtga catgaataat ccggccgatg acaacaacgg cgcaatggac gatctgtggg
    12001 ctgaagcttt gagcgaacaa aaatcaacca gcagcaaaag cgctgccgac gcggtgttcc
    12061 agcaatttgg cggtggtgat gtcagcggaa cgttgcagga tatcgacctg attatggata
    12121 ttccggtcaa gctgaccgtc gagctgggcc gtacgcggat gaccatcaaa gagctgttgc
    12181 gtctgacgca agggtccgtc gtggcgctgg acggtctggc gggcgaacca ctggatattc
    12241 tgatcaacgg ttatttaatt gcccagggcg aagtggtggt cgttgccgat aaatatggcg
    12301 tgcggatcac cgatatcatt actccgtctg agcgaatgcg ccgcctgagc cgttagtgat
    12361 gaataaccac gctactgtgc aatcttccgc gccggtttct gctgcgccac tgctgcaggt
    12421 gagcggcgca ctcatcgcca ttattgccct gatcctcgct gctgcctggc tggtaaaacg
    12481 gttgggattt gtccctaaac gcactggcgt taacggtctg aaaattagcg ccagtgcttc
    12541 gctgggcgcg cgtgaacggg ttgtggtggt cgatgtggaa gatgcacggc tggtgctcgg
    12601 cgttaccgca gggcaaatca atctgctgca taaacttccc ccttcagcac caacggaaga
    12661 gataccgcag cccgattttc agtcggtcat gaaaaacttg cttaagcgta gcgggagatc
    12721 ctgatgcgtc gtttgttgtc tgtcgcccct gtccttctct ggctgattac gcccctcgcc
    12781 ttcgcgcaac tgccgggtat caccagccag ccgctgcctg gcggtggaca aagctggtcg
    12841 ctcccggtgc agacgctggt gttcatcacc tcgttgacgt ttattccggc aattttactg
    12901 atgatgacca gtttcacccg catcattatt gtttttggtt tattgcgtaa cgcgctggga
    12961 acaccctccg cgccacctaa ccaggtattg ctggggttgg cgctgttttt gacctttttt
    13021 attatgtcgc cggtgatcga caaaatttat gtcgatgcgt accagccatt cagcgaagag
    13081 aaaatatcaa tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg
    13141 cgtcagaccc gtgaggcgga tttagggttg tttgccagac tggcgaatac tggcccgttg
    13201 cagggacctg aagccgtgcc gatgcgcatt ttgctcccgg cctacgtgac cagcgagttg
    13261 aaaaccgcat ttcagatagg cttcacgatt ttcatccctt ttttgattat cgacctggtg
    13321 attgccagcg tgttgatggc attggggatg atgatggtcc cccccgccac cattgctctg
    13381 ccctttaaac tgatgctgtt tgtactggtg gatggctggc aattgctggt cggttcgctg
    13441 gcgcagagct tttacagcta gagaggcaaa atgacacctg aatcggtcat gatgatgggg
    13501 actgaagcga tgaaagtcgc gctggcactg gctgccccgc tgttgctggt ggcgctggtc
    13561 acgggcctta tcatcagtat tttgcaggcc gccacgcaga tcaacgaaat gacgctgtcg
    13621 tttattccaa aaatcatcgc cgtatttatt gccattatta ttgccggacc gtggatgctc
    13681 aatctgttgc tggattacgt ccgcaccttg ttcactaacc tgccgtatat catcgggtag
    13741 cccgtacaat gatgcaggtg acaagcgatc aatggctttc ctggctaagt ctgtacttct
    13801 ggccgttact gcgcgtgctg gcgctcattt ctaccgcgcc aattctgagc gaacgcagcg
    13861 tgccgaaacg ggtaaaactg ggtctggcaa tgatgatcac gttcgctatt gccccatcat
    13921 tacctgccaa cgatgttcct gttttttcgt tctttgctct gtggctggcc gtgcagcaga
    13981 tcctgatcgg cattgcgctt ggtttcacca tgcaatttgc ctttgccgct gtgcgaaccg
    14041 ctggcgaaat tatcggtctg caaatggggc tgtcatttgc gacgtttgtc gatccggcca
    14101 gccatctcaa tatgcccgtt ctagcccgta tcatggatat gctggcgtta ttgctgttcc
    14161 tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt cacaccctgc
    14221 caattggtgg cgaaccgttg aacagcaatg cgtttctggc actcaccaaa gcagggagtt
    14281 tgattttcct taacgggctg atgctggcgt taccgctcat tactctgctg ctgaccctga
    14341 atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt gttattggat
    14401 ttccattaac tctgactgtc ggtatctctt taatggcggc attaatgccg ttaattgcgc
    14461 ctttttgcga acatttattc agtgagatgt ttaatttgct ggctgatatt attagtgaat
    14521 tgccattaat ataattccgt aacgtttatc atgttatcct aaggattatc cgaaaaataa
    14581 tacctacgaa catcttccag gatactcctg cggcgaaata tttgttttaa gctcactcac
    14641 atatcgcaac atttacttta ctttaagaca attccaggca aattatacaa cactttacgg
    14701 gatagtaagt ccgcctgaaa aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg
    14761 tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga gtgagggtat
    14821 gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt aaccgggtat
    14881 ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt
    14941 gccatagctt gtgattcaca gcgcccttca gtggtgttta ttaatgagga ctgtttcatc
    15001 catgatgctt ctaacagtca gcacatcaag cacatcatta atcaacatcc caatacgtta
    15061 tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt ggtcagaaaa
    15121 aatttattga taagttctaa atcgattaaa ccggaatctc tcgacgatat ccttggcgat
    15181 attctgaaaa aagagacaac gataacctct tttttaaata tgccgacgtt atcattgagc
    15241 cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat tcaaatctct
    15301 gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat taaacgtaag
    15361 atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga taatgtgact
    15421 aatggtattt ttgtcaacat gcgctaacac attctgactg gtggtttccc accagtcagg
    15481 ctgaataaga ttactctgct ttctccacaa agataccgtc ctgatgccct gcttcattaa
    15541 agaaccagat gccgagcggg tagtcttcca gcgaaaccag gtacattgtg ccttcactaa
    15601 actcctcaac tgccagtacc acgccaggac gacgcggacc gccatccgtt ttgactgtta
    15661 cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta gaacaatttc
    15721 gtttttctgg cagcgccagg cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag
    15781 agatgccagg cggacttaac gactggcggc aacaacagag taacggttgc gaggaaagat
    15841 gatgaaaacc gcaaaagagt acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga
    15901 tgccctgctg gcggcgatca atgaaattag cgaaagcgaa gttcatcgca gccagaacga
    15961 ttctgaacac gttagcgtcg atggacgtga atatcataca tggcgtgaat tggcggatgc
    16021 cttcgaactg gatattcatg acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa
    16081 aaaaatcccg accctgaggg ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa
    16141 attcgttaca ccaggaaatc tgatgtgttc atcaccttat ccgcaatttt tttcgctgac
    16201 aagaaaatat tcgcgatatg aatgattaac gctatttatg aatagaaatg tgacgcacgt
    16261 caccccttgg tggctctggc gtcagcggga tcgctcccga aaaaatcatc tttgataatt
    16321 tgtgctgttt tgagaatcat cctggtaagg ggagaactgc gcgaccatgt tttcaattca
    16381 acaaccacta ctggttttta gcgatcttga tggcaccctg ctggacagtc atagttatga
    16441 ctggcaacca gctgccccct ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct
    16501 ctgtagcagt aaaacttcag cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg
    16561 gttaccgctg attgccgaaa atggcgcagt gatccagctt gctgaacaat ggcaggatat
    16621 agacggtttt ccacgcatca tctcaggtat tagccatggc gaaatcagcc aggttttaaa
    16681 tacgctacgc gagaaagaac attttaaatt cacaactttt gatgatgtcg acgatgcaac
    16741 catcgccgaa tggacgggat taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc
    16801 gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg gcacaattta ccgctcgtct
    16861 gaacgaactg ggcttacagt ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc
    16921 tgccggaaaa gatcaggctg ccaactggat tatcgcgacc tatcaacaat tgtcaggcaa
    16981 acgcccaacc acacttggcc tgggcgatgg gccaaacgat gcgcccttac tggaggtaat
    17041 ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg gggcatctgc atgatgagga
    17101 tccggcccac gtctggcgaa cgcagcgtga aggaccggaa ggatggcgtg aagggctgga
    17161 ccattttttc tccgcccgtt aagcgttatc gctcgcgaat acccgattac gcccagcctg
    17221 tttagcgaga taaagccgac ggtcggccag tgactggagt tgttcaaaat cataatcacc
    17281 ggtttcctcg ctgctactta cccccaacga ggcactgatg cgtatcgtcg tactcttggc
    17341 aatcaacatc tctttttcat ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc
    17401 cgtcagactc gcgcctggca gaatcacaca aaactcctca ccaccgaccc gcccggcaac
    17461 gtcctgcgca cgcaaggaac tgctaattaa tccggcagca tgagaaagaa cacggtcgcc
    17521 cgcctgatga ccaaagcggt cattaatcgc tttaaaatgg tcaagatcga cctgaatgac
    17581 agaaaaagga tattggtgcg tctgacacaa tttagcgagc ggacgggctt tttcgaacag
    17641 tgcgccacga ttatataaac gggttaaggt gtcgtgccac gcctgccact gcaacgagct
    17701 ttgcagaaca tacatgttgc tgaccatccg gcgaatcaca taccaggaga tgagtaacat
    17761 ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg atactgccga aatcgccgcg
    17821 cacgccttcg cttaacgtat ggacgcgcac cagcacaccg tcgaaatggt tcagacgttc
    17881 ccagctaaca tagcgactgt ccatacgaat gccgccccgc gtgtcatgtt ccattgcctg
    17941 cgccagcaag gccagttcac gagggtcaaa aatattccct gttggatggt caggattgga
    18001 agaggtcaaa aatctcagct tactgtcata gagctgatac tcaccatcga ggtttttatc
    18061 gatggcgttt cttaaaaatt gctgcatggt acgcacggga atactcatcc ccagcacgcc
    18121 ataccagtag ttattactgt ctaccggaac actgacggta acctgcggtt cagtattgct
    18181 ggcgtgttcc ggttgcgaag tgaaccagcg taccgcgcgg tgacgatttt ctcgttgcga
    18241 atggccgata aaccagggtt gggtgacata gccgtaataa cgcgttggta cattgcgcgt
    18301 aaacaaggtc ggctgcgtcg aaacgtaaaa tccggcacgc gagacataca tcgcctgttc
    18361 aaccatcgac gaggtgttgt gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt
    18421 aatttcattg tcgaggcttt cattttcgcg agacaggaga ttcccctcgc tgactaatgc
    18481 atccgatacg ccattgactg acagggtgcg tcgtcggttg agttcgattt gccaggcgtg
    18541 ctcgtcgcga tgctgttcga actcggttac agcatcacgc agagaggtga aatccagtgg
    18601 cgctacgaga gcttcgcgca tgccattacg caaaaagatc agtttgtcga cgttatactg
    18661 caaatgctta tcgagcgcgt tggctacgtt ttccagatga ttacgctggc tggagatata
    18721 ggcatcttcc agcactacca cttcacgcta ggtcagcaag gtggaaaaaa gcaataccac
    18781 gataaagcag agattaacga catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca
    18841 gctctggttt tccatttttg tctcgtgctg caccctgacg actcccaacc tgctttttta
    18901 tgattctggc ataacctggc gatagcgtac cgcaaaacag cgataatttt ataatggtgg
    18961 catgataaga cgcgactatc attcattgca ggcattggca caccaggccg gatgtgatcc
    19021 ggcctggtgt taatgatacc ctttcaaact tcttccgcag aacgactccc ctcccccgga
    19081 acaagctcgg tttcatgaaa agcttcccgc ttgaccccgt aaccgtcata ccaacgacac
    19141 tcaaccatac cgctggagta tccagtgaca atcatccgcg ggccgccctc tttaaccgta
    19201 acttcctcac taaccataaa gctcatactc gcctcctttt ttcgagtgaa acttgttcac
    19261 cttagttgaa gatggcgaat tttgcataat agcctgtgcg agtattaatg tgcctgaccg
    19321 cgtattttcg ctacggcttt cactcccagc accacgatgc cgccgatgat aaatccaaga
    19381 atcagattta aaacagtcgg taatatcatc gccaccactg cactttgctg cccggcgaaa
    19441 tgttcaatgg cgtgatgcag cggcgcaata ccatgtacca caatcccgcc gccgacgagg
    19501 aacatcgcca gcgtgccgac aatcgataat gctttcatca accagggcgc aatgatcaat
    19561 aaccctttac ctaatgcctg catcagcgcg ctggattttt ccgccagcca ataccccagg
    19621 tcatcaatct taacgataac ccctaccaga ccgtagacgc ccacggtcac taccagcgcg
    19681 atgcctgaaa gcaccagcac ctgattaagc aacggcgctt cggccacaat ccccagcgtg
    19741 atggcgacga tttccgcaga caatataaaa tcggtacgaa tcgccccttt tattttgtcc
    19801 ttttcaaact tcagcggatc ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc
    19861 ggatcttctt tatgtttacg cgcctccagc atatgcagca ctttctctac tccttcaaag
    19921 cagagaaacg cgccaccaat catcaacaga ggcgtaatcg cccacgggat aaacgcactg
    19981 atgatcagcg ccagcggcac cagaatcact ttattaatca gcgatccttt cgccacgccc
    20041 cagaccacgg gaagttcccg gttggcccgc acgcctgaaa cttgttgcgc attgagcgat
    20101 aagtcatccc ctaatacacc ggcggttttc ttcgccgcca gtttgcccat cacggagata
    20161 tcgtccagca gtgtggcgat atcatcgagc aacgtcagta aactactccc ggccaaaagc
    20221 attccctctc atttttattg ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat
    20281 acttgcttca caggtcaaca tttttttaac aatcaagtgc aattatatct ctcgccagca
    20341 gaatgatttt cacgtttact atgacccacc tttttatttc caccgtgagg gattatgcgt
    20401 ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca
    20461 acctattttg tcattcggat tggcgtggaa agctggcctc cgttaatgat ggcaggcgtg
    20521 cgattcctgg cagccggtat tttattgctg gcatttttgc tactgcgcgg acacaaactc
    20581 cccccgctac gtccgctgct taatgccgcg ctgattggcc tcttattgct ggctgtcggt
    20641 aatggcatgg tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc cgccgtagtg
    20701 gttgcaaccg tgcccctctt taccctgtgc ttcagccgcc tgtttggcat taaaacgcgc
    20761 aaactggaat gggtgggtat tgccattggg cttgccggaa tcatcatgct caatagcggt
    20821 ggaaatttaa gcggcaatcc gtggggcgcg attctgattt taatcggctc gattagctgg
    20881 gcgtttggct cggtttatgg ctcgcgcatt accttacctg tagggatgat ggcgggtgcg
    20941 attgagatgc tggcggcagg cgtggtgtta atgatcgcgt cgatgattgc aggtgaaaaa
    21001 ctgacggcgc tcccttccct ttcaggcttc cttgcggtcg gctatctggc gctgtttggt
    21061 tcgattatcg ccatcaacgc ttatatgtat ttaatccgta atgtcagtcc ggctctcgcc
    21121 accagctacg cctacgttaa cccggtggtc gcggtcttgc tgggtacggg actgggcgga
    21181 gagacgctgt cgaagattga atggctggcg ctcggcgtaa ttgtcttcgc ggtggtactg
    21241 gtcacgttgg gaaaatatct cttcccggca aaacccgtag ttgcgccagt tattcaggac
    21301 gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac cttcgccgca
    21361 gatccactct tccagacgct cggtaagcgc ttcatccgtc agcttctcgc gcccacgtaa
    21421 tgcacactcc cagacaatca gtacgcgcca gccgagttcc tgcaaacgac tgatatcgcg
    21481 gcgatcgcgt tcaacatttt tacctatctt ctccagccag aactcggttc gagtcgcagg
    21541 cactttaaac agatagcagt gatgatgatg ccagaagcag ccatgggtaa atatcacgca
    21601 gcgatattca tcaacgacaa aatccgggct tccgggcaga ctggcgtcct gaacgcgaaa
    21661 cgccaggccc tgcccggtta acagactggc gaggcgcttc tcaatcgccg tatcacgcgt
    21721 ggcaatcgcg cgcatatttt tgctgcgagt ggccttatcg tgaacgtcgg ccatgttgtg
    21781 cctcttgctg acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt tttgccaccg
    21841 cggcaaagac cggcacgacc accgagttac cgaactggcg ataggcctga gtgtccgaaa
    21901 ccggaatgcg gaatttcgct tctcccggcg cttcaaaacc cattaagcgc gcgcattccc
    21961 gaggcgttaa ccgacgtgga cgatgttgct gattcagcgg atcgtcaaaa tctttctcac
    22021 ccgtggccat atcccagccg cgatcgatta aaatttccgc gccatctttg taataacgcg
    22081 cagacagcgt acgcgtgacg ctttgcggat tgttcggata aaccattcca taaccgaagc
    22141 cgttaccgcg cgcctgatgt tttttcgcat atcgatagag gtacttccac agcaccggcg
    22201 tcaggatata tttcgcctcg accatcgggt ccaacagctg cgccagcgtc actcgctgtg
    22261 cagggaaaca ttcgctgata tcacgcagag taaaatcggc tttcagatta agatcgcgac
    22321 gaaaacccac cagcacgatg cgttcacggt gctgcggcag aaaatgttta ccatcgatga
    22381 ttttcggatc gtccggcccg ttatcttctg catcagccac gtcatagccc agttcgtcca
    22441 gcgtctgcat gatgatgcgg aacgttttac cctggtcgtg acttttcagg tttttgacgt
    22501 tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat gcgtaccaca tcaaaaaaca
    22561 gcgtgccctg ggtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc tttttcgata
    22621 cgccagccag cgaaaatggc tgacaaggga aaccggccag taaaacatcg tgttcaggaa
    22681 tgtgttgacg aatatgttcc gccgccgcct catcactcac gccttcttta tggctgaggg
    22741 tgatatcgcg gatatcttca ttaaaatgat gcgtcgccgg atcgcaataa tggttggctt
    22801 tataagtgcg taccgcatgt ttgttccatt cgctggtaaa cacgcattgt ccgccaatcg
    22861 attcaaaacc gcgacggatg ccgccaattc cggcaaatag atcgataaag cgaaacgcat
    22921 aatgcggatg atgtgccggt ggtttgggta acaacgtttg cagatgggcg aactctttct
    22981 cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt gccgcgctcc
    23041 agtgattctc acccacacca ttaagctgcg ccaccagcgt tttaacatca taaatttgca
    23101 gcagtttctc cagcattgcc tgtgcggcat tcccggtgct gtatgaatcg gttactgata
    23161 tattttcctg catagattta accggccatc tgagatggcc gacaggttaa cataattaca
    23221 gccatttagg gccacgcgct tatcccgcgg gtgaaaacgc atctatcacc ttgtgatcga
    23281 caccttcatt ctcccccgcc agttcggcac tgagcttcgc cataaactcg actaaaaaat
    23341 gcgcgttgtg ctgcgccagc tgcttgcctc ttgcggtttg catggtttgc ggtagcttca
    23401 gcaacttagt ctggaaatga tccagcgcgt agcgtttatc atcaagcggg cgatgctgtg
    23461 cgaacggatc ttcaccatca aacagcgcca cgcccaatgc ccctgaaacg gcaaacacgc
    23521 gcgccaggcc aatcgccccc agggcttcca gccgatccgc atcctggacg attttagctt
    23581 ccgttgttaa gggggcgatt tgcgcgctga aactgtgagc ggcaatggca tgacaaacgg
    23641 cctcgatttt ctccgccgga aattgctcga actcttcacg gagcaggcga cgcgtctctt
    23701 ctgccgccag gattgaagaa cgctgccttt gcggatgatt tttcgccagg ctcacaatat
    23761 cgtgaaaata acaggcggtt aaaatcacca gcatatcgac atcgtcatct gccgccagct
    23821 tctgtgccgt agcccagacg cggcgaaaat gacacacgtc atgggccgcg tcctggtgct
    23881 gatgatggtt tttcaaccag ttttcgaact gtgcctgcca gtgttgtaag tccatacgcg
    23941 ctccgttgcc aaagagtcgc caccttagca gtttttattc atagcgtctg ctacgtgcgg
    24001 gacgaggtcg ggtgtaccag gcaatgctgc ctaacaatgc cccgaccaga gccataaata
    24061 aaaatccgac cagtgcccct aaccactttc ccgcggtaga gcctaaatcg ctttcaaatc
    24121 ccgacacggg ggcatcgggg atccggctca tcacccagat gtaacgcgcc attgcccaga
    24181 cgaaatagcc acaccatgta tagaaagccc acaaagccgg tttgccgcca gggctgcgag
    24241 agagtctctc ttccatcttt gtcgaaccgt attccatttt gagataaccc ggaaaattca
    24301 gacttagtac tattatgtga taaaagtcac atttttccac attggatgag ccagtcaaga
    24361 ctcaatcatt ttttgaacaa atagataaat tttgcgcaac ggcagtgaat acaggaaaaa
    24421 tcgttttgag cgattttatt tatctttgat atcggcttta ttaattcatt taatcaatat
    24481 attagcactg attacaatta taccttttta ttattctgtg tcatgatgct tccgttatta
    24541 gccttttatc gtcttgttta tattttttgg gccggcatga tgccggcttt tttttatgcc
    24601 ttcattaatg tgcgccagat cacaccagcc gtttggcgca acaatcattg atacccccta
    24661 tgtttccgct caactcataa catattgata tacatttata atttcatgaa tatttatatt
    24721 tagaattcat aattctgaat tatattaaat agattagaaa cattttgaaa tcttttaaat
    24781 acatttgttg catgtaatcc ttaaaataaa atgaacttca tagaatagta tccaaatgtg
    24841 cttttttttg gataacggca cttattgata tattcatgaa gattataatc acaagggaat
    24901 acataatgaa aagaaaagtt ctggcaatgc tggtcccggc gttattagtt gctggcgcag
    24961 caaatgcggc tgaaatttac aataaagatg gcaataaagt tgatttctac ggcaagatgg
    25021 ttggcgaatg catctggtca aatactgatg ataataacag cgaaaacgaa gatacctcct
    25081 atgctcgttt cggtgttaaa ggtgaaaccc aaatcaccag cgaactgact ggttttggtc
    25141 agtttgaata caacctcgac gccagcaagc ccgaaggctc taatcaggaa aaaacccgtt
    25201 taaccttcgc aggtttgaaa tataacgagt taggatcttt cgactatggt cgtaactacg
    25261 gtgttgctta tgatgccgca gcttataccg atatgttagt tgaatggggt ggtgattcct
    25321 gggcttccgc tgacaacctc atgaacggtc gtaccaacgg tgttgcaacc taccgtaact
    25381 ctgatttctt tggtctggtt gatggtctga attttgctgt gcaatatcaa ggtaagaaca
    25441 gcaatcgtgg cgttactaaa caaaacggtg atggctatgc gttgtctgta gactacaaca
    25501 tcgaaggttt tggttttgta ggtgcatata gcaaatctga tcgtactaat gaacaagctg
    25561 gtgacggcta cggtgataac gctgaagtgt ggtcattagc agccaagtat gatgcaaata
    25621 atatctatgc agcaatgatg tacggtgaaa cccgcaacat gaccgttttg gctaatgatc
    25681 attttgcaaa taaaacccaa aactttgaag ctgttgtaca gtatcagttc gacttcggtt
    25741 tacgtccgtc tttaggctac gtatattcca aaggcaaaga tctttatgct cgtgatggac
    25801 ataaaggtgt tgatgctgac cgcgtaaatt atatcgaagt tggtacctgg tactacttca
    25861 ataagaacat gaacgtctac acagcataca aatttaacct gctggataaa gacgatgcag
    25921 cgattaccga tgccgcaact gatgaccagt ttgcagttgg tatcgtctac cagttctaat
    25981 cagccaacgc gccgcggcgt gttactgtaa aacataacac tagcctgagt tcgtccctca
    26041 tcgaactcag gcttttttat tggcatttca tctccatctg cggcacacgc aattgccaaa
    26101 gcatatcgat acagcacctt accctgcccc tcctgccaca ataacatcta ccagcgactc
    26161 ttccagccaa caccccaaat gcacattctg atgaattacc ccgtaacaaa cacgcttccc
    26221 aaatagaaat ctttgcaaaa atagtgcatg aaataagctt tttctctacc aatccgcgat
    26281 ataaatattt atcagcacca caatattcca catcactaaa tctctcccca ccgccccgca
    26341 tctctgataa tggtctaaaa tcattgaggc cacttgcgac gacgttgctc aataaggaaa
    26401 atttggcgtg aataatattc aacaccagtg cagcatcatc attacatact tacacccgcc
    26461 gactaccgct ttcgatatat tgactcaatt ttccagcctg gttaaaaata gcgaccagct
    26521 cagtcgcaaa tatagtgact accctaacta agcaacaata aggaatacac tatgactgtt
    26581 caaacaagta aaaatccgca ggtcgatatt gctgaagata atgcgttctt cccttcagaa
    26641 tattcgctta gccaatatac cagtcccgtc tctgatcttg atggcgtgga ctatccaaaa
    26701 ccgtatcgcg gtaagcataa aattctggtg atcgccgcgg acgaacgtta tttgccaacc
    26761 gataacggaa aactgttctc gaccggtaac catccgattg aaacgttgct gccgttgtat
    26821 catctccatg ctgcaggttt cgaattcgaa gtggcgacca tttccggtct gatgaccaag
    26881 tttgaatact gggctatgcc gcacaaagat gaaaaagtaa tgccattctt tgagcagcat
    26941 aaatcgttgt tccgcaatcc gaagaaactc gcggatgttg tcgccagcct caacgctgat
    27001 agcgaatatg cagcaatctt tgttcctggt ggtcatggcg cacttattgg tttacctgaa
    27061 agccaggacg tagctgccgc tttacagtgg gcaatcaaaa atgaccgttt tgttatctcc
    27121 ctttgccacg gcccggcggc ttttctggcg cttcgccacg gcgataaccc actgaatggt
    27181 tattccattt gcgcattccc agacgccgca gacaaacaaa cgccagatat tggctatatg
    27241 ccaggtcatc tcacctggta cttcggcgaa gagctgaaga aaatgggcat gaacatcatt
    27301 aatgacgaca tcaccgggcg agtacataag gaccgtaaac ttctcaccgg cgacagtcct
    27361 ttcgcagcga atgcgttggg taaactggcg gcgcaggaaa tgctggcagc ttacgcgggt
    27421 taatccctgt tacgtttgaa tgtaagcgca taattaatcg cagccgcgtt agtactcacg
    27481 acgtggctgc gttttttcac ggttaattta tggcgtactg aagccctatg ttaatttctt
    27541 tgcggtaacg taattcggaa cacattatgc ttattgagat agtgatatga agcacttccc
    27601 ccatgcaatt cggcaatcgc tttgactaaa gaaagaccaa gtccctgacc tacggaatga
    27661 cgcgaattat ctccccgcca aaatctacgg aagagttttt caggctcatg tattttcgtt
    27721 ccaggactgg cgatatcaat attaagatag ccgttggtat caagaaaact ggttatatga
    27781 atacgcgatt tttctggtga atatctaatg gcattaacaa taagattcga taacattcgt
    27841 tgcagtaaaa ttttatccgc aaagatttgc tgattgcact cgaccttaaa gcaaatctct
    27901 ttctcgtctg aaagatattc aagataatcc aacaaatttt cgacttcctt attgagagaa
    27961 agcgagtcca gtttcaccaa aacattgttt ttatctgccc gggcaagaaa cagtatgttc
    28021 tctgttaacc gcgaaatatt ttccaactct tcaatgttac cggcaattgt tttttgatat
    28081 tcagcgatac ttctggtttg actgagcgta acctgattct gacccagtaa tgcattaatg
    28141 ggcgttctaa gttcatgagc gagatcgtca gcaaattgac ttaggcgttc aaaatctttg
    28201 actaaggctt gatgcatttt attcaacgcc tgcccaagag gttttagttc tctcggtaat
    28261 gcgctaacct caacaggctc tcggctatag ttataattca gcgcttccgt tacaccactc
    28321 aactttttga tctctcgtaa tcccgttctg attaacagcg gacttaatac tgagcaaagt
    28381 acaatggcga caatgcaaat tataatgcta ttaattttat actgttcaag catgttatgt
    28441 ctggctgaag ccaatttagc cacagtaacc gttaatgatg gcgaaacttc atcaatatta
    28501 attcgtaaag catcaatctc agtatcatta ataatgcttc tgtaaatgcc agctgcgctg
    28561 attgtctcac tagcaggtat gttatttaac atgtcatcac tgacatttgt ccggttaaca
    28621 attttattga tgccatcacc atgaatgatc aagatatcct gactaacatc catcatccgg
    28681 ttaaagtata caggtaacgt atctggattt accccatcaa ttaacaactg cttgatctgt
    28741 gctgtccggt taatgagtgt tgtatcatcg cgccatttca actcacttgc caggccatta
    28801 tagagagtcc agacaattcc agcgccagca acagacagca gcaatataaa aagcaaggtt
    28861 aaacggacgg ttatagacaa tcttttcatt atttttttac cgctacgaat gaatagccca
    28921 tcccccggat tgtggcgatt agcttttcag gaaaaggatc atcaactttt gcgcggagcc
    28981 tgcgaatggc gacatccacc gtattggtat cactatcaaa gttgattccc caaatttcac
    29041 tcgcaataac cgttctgggt ataatttcgc cagctctgga ggccagtagc caaagtaact
    29101 gaaactcctt gcgcgtcagt gtaatactga tattgtccct gctcacactt tgacttacag
    29161 agtccattct taagccactg atttctaatg ttgaattcaa agcgtgatgt tgccttaatt
    29221 gtgcccgaac ccttgccagc aactcagaaa atgaaaaagg ttttaccaga taatcatttg
    29281 ctccactgtc cagccctctg actctgtcat cgacagaatc ccttgcagta aggcaaataa
    29341 caggggtttg ctttgctgtt cttaacgttt gtaagatctg ccagccatcc atacccggaa
    29401 gcataatatc cagaattatc aatgcataat catccttcag cgcaagataa agcccatctc
    29461 tgccatcaga aacggcatca atgacataac ccgcttcgga aagcccctgc gttacccact
    29521 cctgggtcct ttgattatct tcaataagta aaatcttcat gaaaataata tgccataaat
    29581 catatgttat agaaacagcc tggttcatta caaaattgta atgctgctgt aaggttaccc
    29641 tggccgcttt ttcgctatcc tcaaaactca ttcacatgac aaggatataa acatgttaaa
    29701 gcgttattta gtactctcgg tagcaacggc agcattttca ttaccttctt tggtttatgc
    29761 cgcacaacaa aacattctta gcgtgcacat tttgaaccag caaaccggaa aacccgctgc
    29821 tgacgtgaca gtcactcttg aaaagaaggc ggataacggc tggttacaac ttaataccgc
    29881 caaaacagat aaggatggac gaattaaggc actgtggccc gagcaaactg caactacggg
    29941 cgattaccgt gtcgtattta aaaccgggga ctatttcaag aaacaaaatc ttgaaagttt
    30001 cttccctgag atccccgttg aatttcatat taataaagtg aacgagcatt atcatgtgcc
    30061 tttattactt agccaatatg ggtattcaac ctatcgtggc agttaatgca taaatatgta
    30121 aaagcagatc tctgctactg ttagggagga cactccctga cagattaaca gtaaacggct
    30181 cttgctggct aacgacaaaa aagtgtgatg gcttatgaaa aagaatcaat ttttaaaaga
    30241 atcagatgtt acggccgagt cggtattctt tatgaagcgt cggcaggtgt taaaagcact
    30301 gggcatcagt gcagctacac tttctttgcc tcacgctgcg catgccgatc tgcttagctg
    30361 gtttaaaggg aacgatcgcc cgcccgcccc cgccggaaaa ccgctggagt tcagcaagcc
    30421 tgccgcctgg caaaataacc tgccactgac gccagtagat aaagtctccg gttataacaa
    30481 cttctatgaa ttcgggctgg ataaagccga tcccgccgct aatgctggta gcctgaaaac
    30541 cgatccatgg acactgaaaa tcagcggcga agtggcaaaa ccattgaccc tcgatcacga
    30601 tgatttaacc cgtcgcttcc cgctggaaga gcgtatttat cgtatgcgct gcgtggaggc
    30661 atggtcgatg gtggtgccgt ggattggtt
//
LOCUS       DAEVZA010000047        30126 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEVZA010000047 DAEVZA010000000
VERSION     DAEVZA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.047"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1191
                     /locus_tag="LAF92_002199"
     CDS             <1..1191
                     /locus_tag="LAF92_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBI9791201.1"
                     /translation="NILEAARERITWTLNTLPRVCVSFSGGKDSGLMLHLTAELARQM
                     GKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNSLSQYQPEWQC
                     WEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPAAMMIGIRADE
                     SYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTWYANHQSLCNP
                     LYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVSGVKSGGIYAG
                     HDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQKKGIEVPQ
                     TQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERIKGKRQEWGIL
                     CNND"
     gene            1164..1793
                     /locus_tag="LAF92_002200"
     CDS             1164..1793
                     /locus_tag="LAF92_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBI9791202.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1794..2954)
                     /gene="ybdL"
                     /locus_tag="LAF92_002201"
     CDS             complement(1794..2954)
                     /gene="ybdL"
                     /locus_tag="LAF92_002201"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBI9791203.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3063..4151
                     /gene="hcxA"
                     /locus_tag="LAF92_002202"
     CDS             3063..4151
                     /gene="hcxA"
                     /locus_tag="LAF92_002202"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBI9791204.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTV
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4161..4358)
                     /locus_tag="LAF92_002203"
     CDS             complement(4161..4358)
                     /locus_tag="LAF92_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBI9791205.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4540..6645)
                     /gene="cstA"
                     /locus_tag="LAF92_002204"
     CDS             complement(4540..6645)
                     /gene="cstA"
                     /locus_tag="LAF92_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBI9791206.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6826..7239)
                     /gene="entH"
                     /locus_tag="LAF92_002205"
     CDS             complement(6826..7239)
                     /gene="entH"
                     /locus_tag="LAF92_002205"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBI9791207.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7242..7988)
                     /gene="entA"
                     /locus_tag="LAF92_002206"
     CDS             complement(7242..7988)
                     /gene="entA"
                     /locus_tag="LAF92_002206"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBI9791208.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(7988..8845)
                     /gene="entB"
                     /locus_tag="LAF92_002207"
     CDS             complement(7988..8845)
                     /gene="entB"
                     /locus_tag="LAF92_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBI9791209.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8859..10469)
                     /gene="entE"
                     /locus_tag="LAF92_002208"
     CDS             complement(8859..10469)
                     /gene="entE"
                     /locus_tag="LAF92_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBI9791210.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTRQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10479..11654)
                     /gene="entC"
                     /locus_tag="LAF92_002209"
     CDS             complement(10479..11654)
                     /gene="entC"
                     /locus_tag="LAF92_002209"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBI9791211.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPQQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11843..12799
                     /gene="fepB"
                     /locus_tag="LAF92_002210"
     CDS             11843..12799
                     /gene="fepB"
                     /locus_tag="LAF92_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBI9791212.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12803..14053)
                     /gene="entS"
                     /locus_tag="LAF92_002211"
     CDS             complement(12803..14053)
                     /gene="entS"
                     /locus_tag="LAF92_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBI9791213.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14164..15168
                     /gene="fepD"
                     /locus_tag="LAF92_002212"
     CDS             14164..15168
                     /gene="fepD"
                     /locus_tag="LAF92_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBI9791214.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFTGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15165..16157
                     /gene="fepG"
                     /locus_tag="LAF92_002213"
     CDS             15165..16157
                     /gene="fepG"
                     /locus_tag="LAF92_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBI9791215.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16154..16969
                     /gene="fepC"
                     /locus_tag="LAF92_002214"
     CDS             16154..16969
                     /gene="fepC"
                     /locus_tag="LAF92_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBI9791216.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16966..18099)
                     /gene="fepE"
                     /locus_tag="LAF92_002215"
     CDS             complement(16966..18099)
                     /gene="fepE"
                     /locus_tag="LAF92_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBI9791217.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18315..22196)
                     /gene="entF"
                     /locus_tag="LAF92_002216"
     CDS             complement(18315..22196)
                     /gene="entF"
                     /locus_tag="LAF92_002216"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBI9791218.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLVQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGG
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISSEAFEKIGPIIRATLNR"
     gene            complement(22193..22411)
                     /gene="ybdZ"
                     /locus_tag="LAF92_002217"
     CDS             complement(22193..22411)
                     /gene="ybdZ"
                     /locus_tag="LAF92_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBI9791219.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22414..23616)
                     /gene="fes"
                     /locus_tag="LAF92_002218"
     CDS             complement(22414..23616)
                     /gene="fes"
                     /locus_tag="LAF92_002218"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBI9791220.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23859..26099
                     /gene="fepA"
                     /locus_tag="LAF92_002219"
     CDS             23859..26099
                     /gene="fepA"
                     /locus_tag="LAF92_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBI9791221.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGSWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26274..26894
                     /gene="entD"
                     /locus_tag="LAF92_002220"
     CDS             26274..26894
                     /gene="entD"
                     /locus_tag="LAF92_002220"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBI9791222.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27016..27168)
                     /gene="hokE"
                     /locus_tag="LAF92_002221"
     CDS             complement(27016..27168)
                     /gene="hokE"
                     /locus_tag="LAF92_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBI9791223.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27519..27671)
                     /gene="hokE"
                     /locus_tag="LAF92_002222"
     CDS             complement(27519..27671)
                     /gene="hokE"
                     /locus_tag="LAF92_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBI9791224.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            28113..29231
                     /locus_tag="LAF92_002223"
     CDS             28113..29231
                     /locus_tag="LAF92_002223"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBI9791225.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCENERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29297..29545
                     /locus_tag="LAF92_002224"
     CDS             29297..29545
                     /locus_tag="LAF92_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBI9791226.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29610..29978
                     /locus_tag="LAF92_002225"
     CDS             29610..29978
                     /locus_tag="LAF92_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBI9791227.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
BASE COUNT         7183 a         8395 c         7787 g         6761 t
ORIGIN      
        1 aatattctgg aagctgcacg agagcgtatt acctggacac tcaacaccct accccgcgtt
       61 tgtgtctcat tttctggcgg caaagactct ggtttaatgc tgcatctgac agccgaactt
      121 gcccgacaaa tgggcaaaaa aatctgcgtt ttgtttatcg actgggaggc gcaattctct
      181 tgcaccatta actatgttca gtccctgcgc gagttgtaca ccgatgtcat cgaagagttt
      241 tactgggttg cgctcccgct tacgacgcaa aattcccttt cacaatacca acccgaatgg
      301 cagtgctggg aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc gataaccgat
      361 cctgactttt tctgctttta ccagccaggc atgaccttcg aacaatttgt acgtgagttt
      421 gccgaatggt tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcggatgag
      481 tcctacaacc gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgacgataaa
      541 ccctggacta cggccgcacc aggcggtcat agctggtaca tttaccccat ttacgactgg
      601 aaagtggctg atatctggac ctggtatgca aatcatcaaa gtctctgcaa cccactgtat
      661 aacctaatgt atcaggcagg cgttcctctg cgtcatatgc gaatttgcga accttttggc
      721 ccggagcaac gacaaggatt atggctctat cacgttatcg aaccggatcg ctgggctgct
      781 atgtgcgcac gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgacaatcat
      841 ttctatgggc atcggaaaat cctcaagcca gaacatttag actggcaaga atatgcatta
      901 ttgctgctca atagcatgcc ggaaaaaaca gctgagcatt accgcaataa aatcgccatt
      961 tatttgcact ggtatcagaa aaaaggcatc gaggttccac aaacccagca aggggacatt
     1021 ggcgcgaaag atatcccctc ctggcggcgg atatgcaaag ttttactcaa taacgattac
     1081 tggtgtcggg cattatcatt tagtcctacg aaagcgaaga actatcagcg ttataacgaa
     1141 cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta acgcaggatt
     1201 taacgcaatt tctcgccagc ttgccggaag atgatcgcat caacgcgatt aatgaaatcc
     1261 gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc gttctgtggg
     1321 ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca ccacccgaaa
     1381 aaaaactgct gaaaaaatcc attgaaatag atggttttac ccaacccatt gtggtcacgc
     1441 atacggataa aaatgccctg gaaatcgtgg atggttttca ccgccatgaa attggtaaag
     1501 gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt ctggaaggta
     1561 cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga cgccatcaaa
     1621 tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac gataataaaa
     1681 ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa attaacggtc
     1741 tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gactgtcaaa taactacagc
     1801 tggcgcagtc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa acagagacga
     1861 atcagtttat gagggaaggg atcggcgcaa aacaccgata gcggaatcgc cgctacgccg
     1921 tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagatac ggcgctgtaa
     1981 tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct ttcatttaaa
     2041 gcattcacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag ataatgctca
     2101 ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac cgaaaaggtc
     2161 agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac acaataaccc
     2221 actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac tgcccgctca
     2281 cgcagctgcg gatgcgccag cacactggca tggccctgtt gtgaaaagtt gatgtgctcg
     2341 tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca caaagcggcg
     2401 aaatcagcct gctgccagac agttgcactg gggttatgcg gagtgttgag gatcaccagt
     2461 ctggtgcgct cgcttaacaa tgcggcaaat tcctgccagt caacgcgaaa atgcggtggt
     2521 tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcggggga gtaactgtca
     2581 tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt aatcgccgcg
     2641 tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc tggttgatag
     2701 ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac gccggtcatg
     2761 ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg taaatagcgc
     2821 ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg ctgcgccagc
     2881 gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg tggaatcaga
     2941 gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca ctataacacg
     3001 atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa ggacaacaca
     3061 acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt tcacatccag
     3121 gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc gcggtgtgga
     3181 tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg tttggacttc
     3241 caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta cagcaactgg
     3301 cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc gcactgctcg
     3361 acaccgtgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgct ccgacgatcg
     3421 ccgccacctg cgcagcctgg acaccgctct ccgtctggta taacgatgcc ggacaggcgc
     3481 tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaaccg gagattatcc
     3541 tcaatgcacc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg aaatggtatg
     3601 aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcgg ctggggatca
     3661 ataatgcgca ggccattcgc gacgtcttgt taaacagtag cgaacaggca cttgccgatc
     3721 agcaaaatca gcagttaacg caatcatttt gcgatgtggt ggatgcgatt atcgccgggg
     3781 gtggaatggt cggtggtctg ggcgatcgtt ttacgcgtgt ggctgcggcc catgccgtgc
     3841 ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc aaagtcgctt
     3901 acggaattct ggtgcaaagc gccttgctgg gtcaggatga tgtgctggcg caattaaccg
     3961 gggcgtatca gcgttttcat ctgccgacta cgctggcgga gctggaagtg gatatcaata
     4021 atcaggcgga gatcgacaaa gtgattgccc acaccttgcg cccggtggag tccattcatt
     4081 acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa gtggaatcgt
     4141 ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc gtagcgcgcg
     4201 tcctgccgct cacggaaaaa ctcctcgtag gtcatcggcg tttgatcggg atggttaacc
     4261 cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt cgccgcctga
     4321 cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc taaccatgtt
     4381 gctgaaagag tgccggatgc ggcgtgaacg ctttatccgg cctacaaatg ctatcaacgc
     4441 actcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggggga accctcggca
     4501 ctatgccaac attcgccaac atcccccctc actctgactt tagtgcgcac ctttcgcctg
     4561 cgctacgatc tcctcaacat tttccggcat tggctcatac ggcgtttctt tcgccgttgg
     4621 cttcggctct ttcaatgccg caagtgccgt cttaatcgag aacagtgcca gaaccaccac
     4681 gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca ccagttgcgc
     4741 cagctgtgac tcagtatact gcgacggaat attgccgctg tcgatcattg cctggaactt
     4801 gttagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct gccagcctgc
     4861 ggtcagggta caaatcagta gccaggccgt tggtaccagc gccacccagg cgtaacgttg
     4921 acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg ccagcatctg
     4981 gttggcaata ccaaacagcg gccacagagt gttaataccg cccagcggat cgaccacgcc
     5041 ctgatggagg aagtagcccc acgccagtac gcacagcgcc gttgccagca ggttagcagg
     5101 cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct gcaacataaa
     5161 gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt caaacagaat
     5221 ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt gcaggatgta
     5281 agccatcccc accgccaacg tcggcgcacc gcccgcacgg gaaatgatcg actgttcacc
     5341 cacttcgctg gcaatctggt tgagcgtatc tggtgtaatg ctaaagcccc agctactcac
     5401 cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca ccgccattgg
     5461 gctgttcatg gcaaagtaaa cgcccggatc gatgatacag gcggaaacca gcgccataat
     5521 cgccacgaag gattccatca acattccacc gtagccgata aaacaagcct gcccttcgtt
     5581 cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagccag acaccgcgcc
     5641 acaggcgatg gtgataaaca ggaacgggaa caggttaccg gtccataccg ggccagtgcc
     5701 atcgacaaat ttggtcagcg caggcatggt cagcgtcggg cgcataatca aaatgcctac
     5761 cgccagaccg acgatcgtcc cgattttcag gaaggtagag aggtagtcac gcggggccag
     5821 cagcaaccac accggcagca ccgccgccac aaaaccgtaa cccaccagca tccaggtcag
     5881 ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcgga ctttctgcca cccagccgcc
     5941 agagataatg gcgaaaatca ggaataccag accgatgacc gacacttcac caatacgccc
     6001 cggacgcaga tagcgcaggt agatccccat aaacagcgcc agcggaatgg tgaacgcaac
     6061 ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg ccagtactgc
     6121 aaggataatg accatgatca taaagcaggc caccagcgct atcaccccgg cggttggccc
     6181 catctcttct ttgaccagct cacccagcga tcgaccgtca cggcgcgtag aaacaaacag
     6241 caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc agatcatccc
     6301 tggcaggtaa cccatttgcg ccgccagtac cggcccaacc aacggacctg ctccggcaat
     6361 ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt ccagcccgtc
     6421 gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat ttttggcgat
     6481 atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca ccacaatcca
     6541 cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa atgctcccat
     6601 tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg ttatccgtgt
     6661 gcgtgtccat agatatgtta cattttgttt ctataacatc tttgtacgat ttaacaacac
     6721 ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta actcgatcac
     6781 tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc ccaaaactgc
     6841 cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga cgatttccca
     6901 gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct tgccttcaga
     6961 caccgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc cgtcacgggt
     7021 catcataaat ccggccatcg atcccagcgt ttcagccagc gccgctgacg cgccgccatg
     7081 cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt ccagcacatc
     7141 atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat cgctggtggc
     7201 gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc cagcgttgag
     7261 ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga ggcgaggaac
     7321 aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat gccgagttta
     7381 aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac ccacagcgtg
     7441 cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac gccgctaacc
     7501 gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga tgcgccataa
     7561 gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac aatcgccccg
     7621 ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc accgccgacg
     7681 ttaaccgcaa aagtctgctg ccagtcctct ttgctcagtt ggtctgtcgc gcccatgcgt
     7741 aaaattcccg ccgcattgac cagcgcgtcc agtcgctccg tttcagctaa cagtcgctga
     7801 cacacttgcg ccacctgcgc agcgtcggca acatccatca cttcggtcgc aaagggatat
     7861 tgctcctgag tgaacgcttg atcaaaacct gtaactttcg ctcccgcctc aacaaacgcc
     7921 agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt tttaccgctg
     7981 aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg gtcgggtttt
     8041 tcgccagcat gacaaaatcg atatcaccat gcactttgcg ccagcgcgcc gccagcgcca
     8101 tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac ggttcatcgg
     8161 actcgtccag caacggcagg atcacctcac gcagcgccgc tttgctggcg gggacaggtg
     8221 ctggcagcaa ttcttcggtc atcaccaccc ggccagaacg tccggccaca tatttcagcg
     8281 acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata aacggtttaa
     8341 tatcgcgcat aaatgcgtcg gttgcggtag tcatacagcc aatgtgggca tataccccgg
     8401 taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga gaacgatgaa
     8461 acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc aggcgatcca
     8521 ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca ttcaacagcg
     8581 cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccgga atattgtgct
     8641 gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc atcgggcagt
     8701 tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac gcggcacgtt
     8761 gcggttcaaa ggcccagtca actttattct tagggatatc gtgagactcc ggcagtgcgt
     8821 aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc gcgtgacgcc
     8881 agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg aagtgaatct
     8941 acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg caggaaacga
     9001 cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcgcagct tttttcgccc
     9061 atcagctcat cttccatgct caccagtgcg gcgtagatca ccgccgggtg gcgcagcagc
     9121 aggttttcga tctcttcggc agcgatcttc tcaccgccac ggttgatctg atctttctcg
     9181 cgcccctgca cggtgatgta cccctctgga tcaatagaga tcagatcgcc ggaacagtaa
     9241 aaaccgttgg catcaaaggc gctggcattg tgctgcgggc ttttgtaata gccacggaag
     9301 gtgtatggac cgcgggtcat caggcgtcca acttcccctt gcggcagtgg atttccttca
     9361 gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt atggataatt
     9421 ttctccgcgc tatcatcaag acgggtgtag ttcaccagcc cttccgccat gccaaacacc
     9481 tgctgcaact ggcagccaat ctcagcggga atacgcgccg caagggtggc agaaagacgt
     9541 gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc accttcggtc
     9601 agcgcctgca accacaggct gactgccggt ggcaccagcg cggtgacgtt cacctgatgt
     9661 ttttcaatca atgggaagca aagcgtggcg ctgggatcgg ccgccagaac aaccgttcct
     9721 ccggcaagaa agacgcccag cgatcccggc gaactcatgg cgtagttatg agccgccggg
     9781 atcgcgcaca ggtagcgtgt ctgtcgtgtg aactgacaaa tctcgacgct acgacgcacg
     9841 ctgtagtagt agtcgttatg agtgcgcggg atcagtttcg gtgtgccggt ggtgccgccg
     9901 gaaagctgga aataggccac ttcatcagca ggtgatggcg tggcagtaaa atcctcagcc
     9961 ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgtgcag ttgcaccacg
    10021 cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct aaacagcgca
    10081 tgttggcgat cggcaatcag caatgcgggt tcaatctggc tggcataggc gttcagttca
    10141 ctacgctgat ggctgaacag cgccagcacc ggcgcaacgc ccagtttcag cagcgcgaaa
    10201 aaggtaatat acaattcagc gacgttaccc agttgtacca gcgcggtttc accaggttta
    10261 atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag ctcccggtaa
    10321 ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg gcgagtcaga
    10381 atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg ggcaaactct
    10441 tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc aaaaacgttc
    10501 aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga cgacgcaggc
    10561 acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc gcagcggatg
    10621 gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc gccaaacagt
    10681 tcgcggtcga atggctccag ttcagcaata acctgggtcg cggcctgatg cgggaaaccg
    10741 ctcagcgcgg gggttggatg cagcagacag gccagagtca gtgcgttttc ttgcgaattc
    10801 gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat caattgtgga
    10861 gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat cgcctgagtc
    10921 accagttcat gttcatggcg atctttttct gacgccagca gacgattacc cgcttcgcga
    10981 tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga gctaaaacgc
    11041 tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc gccatcagcc
    11101 agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc cagcaatacg
    11161 ccactatcaa tggcggcgtc agtggtgata tcaatcaacc gtgacaacac cactttgtcg
    11221 acctgcggcg tggcggtaag tgcggcagcg cgagcaacca tctgttcaaa cgtggtttgt
    11281 tgcggaattg cctggcgttc taccacgttc agcgactggc tgcgggtgaa acggcgggcg
    11341 gaagcttgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata cagcgacgaa
    11401 ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat gccctgcgct
    11461 ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg cgaatccccg
    11521 ttcacagccg gttcatcgaa acgggcgaaa catcctgacg tcgtaaaact gcggtacggc
    11581 gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac ttcctcagcc
    11641 agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg attatcattt
    11701 atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg aggtaacgca
    11761 atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag cattaacttc
    11821 attaataaca ggaagctgat ttgtgagact cgccccgctc taccgcaacg cccttctatt
    11881 aacaggactt ttactttcag gaatagccgc agttcaggcc gctgactggc cgcgtcagat
    11941 tactgacagc cgtggcacac atacactgga aagccagccg cagcgtattg tttccaccag
    12001 cgtcaccctg accggctcac tgctggcgat tgatgctccg gtgatcgcca gcggcgcgac
    12061 cacgccgaat aaccgcgtcg cggatgacca gggcttttta cgccagtgga gcaaggtggc
    12121 gaaagaacgc aaactgcaac ggctctatat cggcgaaccg agcgccgaag ccgttgccgc
    12181 gcaaatgccg gatctgattt taattagcgc aaccggcggg gattcggcgc tggcactgta
    12241 tgatcagctt tccaccatcg ccccgacatt aatcatcaat tacgacgaca aaagctggca
    12301 gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg cagagcggat
    12361 tgcacagttt gataagcaac tggcagcggc gaaagagcaa atcaaattac cgccgcagcc
    12421 ggtcactgcc attgtctata ccgccgctgc acacagtgcc aatctctgga cgccggaatc
    12481 agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaagttgc ccgcagggtt
    12541 aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg gggaaaatct
    12601 ggctgctggg ttaaatggcg agtcactatt cctgttcgcc ggtgatcaga aagatgccga
    12661 tgcgatttat gccaatccac tgctcgcgca cctgcccgca gtacaaaaca agcaggttta
    12721 tgcgctggga accgagacgt tccgcctgga ttactacagc gccatgcaag tgctggaacg
    12781 acttaaggcg ctgttttaag cattaactgt cagacgctgt cacctgcggc ggcgtctggc
    12841 gaaaacgtcg caactccacc agcaccagca ataacaacac gccgataatc aacaaaccaa
    12901 aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc gcccaaacca cccagcagcg
    12961 ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca caaaccgtta atccgcccta
    13021 acatcgcttc cggggtttgc gtttgcagca ttgtatattg cagcaacgaa ctaaccgcgc
    13081 tcagccagcc gaacagcgcc agacaaacca cgcctaaaat ccacatcggc atcaggccaa
    13141 acagaccaat ggcgaggaac gatcccagcg tggagagcag catcaataac cctggtcgcg
    13201 cactatgtgc cagcttcccg ctggttaacg caccaatagc cgcgccgagc gggatcgccg
    13261 cgtagagaaa accaatctgt gccgctgaca tctgccagtt gtcagccagc gccggataca
    13321 gtacccgcac cgcgctcgcc atcgtcaata aaccacccag cagcgcaatc ccgcccacca
    13381 gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcacgcg
    13441 gctgcggtgg cggtggcaac gccggaaggc ttaacaacgg tagcaaggta ataaacgtgc
    13501 ccgccgccgc cagcccgtag ttccaggcta cgccaccggt cgctagcaat aaaccgccaa
    13561 tcatgggcga aatcaccgac cccagacgca cggtcaacat ggtgatcgcc ccggcctgca
    13621 ttaagttttc acgccctacc agtgctggtg tcgccgccaa tagcgccgta acgccaagcg
    13681 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    13741 gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    13801 tcactttttt gcgctcatag cgatccgcca gcacaccgcc aaccatcagg ccaacaaaca
    13861 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgc gtcatcatct
    13921 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag
    13981 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    14041 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    14101 acatgctaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    14161 cgtatgtctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    14221 ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc cgcttccgta
    14281 gtgctggagg ccctctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    14341 cgactgccgc gcacccttgc cggtttactt gcaggcggcg cgcttggcct tgccggggcg
    14401 ttgatgcaaa ccctcacccg aaatccactt gccgaccccg gcttgcttgg cgtgaatgcc
    14461 ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc cgcgcaggaa
    14521 caactggcga tggccttcac cggggcgctg gtggcctcat tgattgttgc ctttaccggc
    14581 agtcagggcg gcgggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg
    14641 gcggtgctgg aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa
    14701 ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    14761 ctgatcccgg ttctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    14821 ttgagcctcg gcagtgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagtta
    14881 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cagcggtagt tggcccgatt
    14941 gcctttattg gcctgatgat gccgcatatg gcgcgttggc tggtgggtgc cgatcatcgc
    15001 tggtcgctgc ccgtcacgct actcgccacc cctgccctgc tgctgtttgc cgatatcatc
    15061 gggcgggtga ttgtgcccgg cgagttgcgc gtttctgtgg tcagtgcgtt tattggtgca
    15121 ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    15181 gccgattact catcacctgt ttgctgctta tttccgcctg tgtggttgca ggtatctggg
    15241 gattacgcag cggtgccgtc acgctggaaa cctcgcaggt attcgccgcg ctgatgggcg
    15301 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    15361 cgctgttgat tggcgcggca ctgggcgtca gcggtgcgat ttttcagtcg ctgatgcgta
    15421 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    15481 tggcgatggt gctgtttggt caggacctga cggctatcgc gctgtcagca atggtgggcg
    15541 gcattgtcac ttcgcttctg gtctggctgc tcgcctggcg taacggcatc gacacctttc
    15601 ggttgattat tatcggtatc ggtgttcgcg ccatgctggt ggcctttaat acctggctgt
    15661 tgctgaaagc gtctttagaa acggcgctaa cagcgggttt gtggaatgcc ggatcgctca
    15721 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    15781 ccgccacctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat accgcctgcg
    15841 cgctgggcgt cagcgtcgaa cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    15901 ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca
    15961 ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg ctgtgcggag
    16021 cgctgttact gctggcggcc gatctctgcg cccaacaact gtttatgccg tatcaacttc
    16081 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    16141 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg
    16201 atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac
    16261 ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct taagccgcct
    16321 gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag
    16381 taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat
    16441 caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg
    16501 gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct
    16561 ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgcgcgt ggatcgcgat
    16621 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    16681 cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    16741 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    16801 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    16861 gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag ccggaacgcc
    16921 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    16981 tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca cgctaccaca
    17041 agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc
    17101 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    17161 gatatttgct tttgttaact gctcaacaag atactgacga ttacgtaatt caccgttcag
    17221 ttccgcaacg tcagtgaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc
    17281 gagagaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt
    17341 tttaattccc gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    17401 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    17461 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    17521 atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg taaaacttag
    17581 cgtccaggag gtatacagtg acggttcatc ttttttctta ctggcattgt catcaaccgc
    17641 tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat cgattttcgc
    17701 ctcttttaat tggtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    17761 agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat taacatcgag
    17821 atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa tggcttcggc
    17881 gggagtaata acggctgagc tggtccattt ttgcggcagg atgaaagaga tcagtaaacc
    17941 tgcgcaggca aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaacttc
    18001 gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat actcagaaaa
    18061 atgagcgtca cttccctgtt taatattcaa tgatgacatg gggtataacc tgaaaatgag
    18121 tccaataggg gaaaatctcc acggcgctca ctttaccagt tggtgagaat tatcctaagc
    18181 tgaagtttgt aaattccttc cactgaatgc aacattcact ctatttaaga gaaataacta
    18241 gcaaacttat aaactaaata aatgattaac ccataattat ttcctataat tatgggttaa
    18301 tagataatgt taatttacct gtttagcgtt gcgcgaataa tcggcccaat tttttcaaac
    18361 gcctctgaag atataatatc cacatgcgca caatcctgac gataaatatc cagctcggct
    18421 atccacggcg accaggcgcg ttcgggactc ataccttcct gaagcgtgcg ttcagcaaca
    18481 aacagcgtcg ctttaccgtc aaacggtacg ctatgagcag tcgtcagcag gcgcactgca
    18541 tcagcgtagt tgccttcaat ggtggtaaac aactccgttg aagtacctcc ctgctgtgcc
    18601 gccaggaagg cctcgcgttc gcggttaatc tccgccagca cttccgggtc cagaccatta
    18661 gcttcttttt cctgccagtt ttgcgtttct ggcggccagg tatccagcaa gccaagaaat
    18721 gccacctgtt cgccacgggc acgcagtcgc gccgcaatac cctgcaccag cgtaccgcca
    18781 agggaatacc ccagcaggta ataagggccg tgcggttgtt gctcaagtaa cgttgccaga
    18841 tgcgcttcgc agacttcatc caggtttgtc gctgtctgca tgggtccatg agggcgcggc
    18901 gactgaatgc caataatcga ccattgtgga tcgagataac gcgagagcac gctaaactgc
    18961 caggcaaaac cggatgccgg atggaaacaa aacagcgtcg ggccattacc ttcacgcaac
    19021 ggcagaatgg tttcgaatcc catgcgccgg gagctgtcct cttcaccatc aataatcgtt
    19081 gccagtttgg cgacggttga cgcgaccatc acctgccccg gcgtcacctg acgagcaaac
    19141 tgtcgactta actgcgctgc cagtttcatt gccagtagcg aatgaccgcc aagcgcaaag
    19201 aaatcagcat cggcatcctg cacgtcgcaa cccagcaacg acgcgaatgc cgcggcgata
    19261 atcgtttcac tgcccgcttt cggtgcacgt cccgacgctt gtgtcttcag ttcaggcaac
    19321 ggtaaggctt tgcgatccag cttgccgttg gcgctaagag gtaactgtgg aagttgcagc
    19381 agaaccaccg gcaccatatg cggcggcaat gtttcgcgaa gctgtgcctg taatgcgctg
    19441 gtatccaacg gcagacctga ttgcgacacc agatagccca ccaactgacg cgcatcacca
    19501 ccggtggctg ccgccaggtt aatcacacag gcgtgggtaa cggcttgttt gacatccggc
    19561 agcgcctgca tcacgcgatc gatttcgccc agttcgatac gctgcccgcg aatttttagc
    19621 tgatcgtcac tgcgcccgag gtactccacc gcgccgttat ccagccagcg ggcgacgtct
    19681 ccggtacgat acatccgttc acctggggca aaaggatcgg caataaaacg gctggcggtc
    19741 agatcgggtc gtccaagata cccctgcgcc agttggatgc cggtgagata gagatcaccc
    19801 gccacacccg gcggcaccgg atgcatcatc gcatcaagaa tacgcagacc cgtattccat
    19861 accggataac caatcggcac actgctgccg cgcacctgtg ccagttcctc gccaaaagcc
    19921 ggataccagc tgacatctac cgccgcttcc gtcgggccat acaggttatg caacggcgcg
    19981 ccggttaact gttgccattc gcggcataaa tcagccggta aggcctcacc actacagaaa
    20041 acctgtttca acgtcgcgca attctggcga gcggtttgcg gcgtcagcga tgcaacaaat
    20101 gccgccagca tcgacggcac aaagtgcgtg gtcgttacgc catattcggc aaagaattgc
    20161 tgcatagcga gcgggtcacg gtgcgcttcc ggttcagcca tcaccagttt tgcccctgcg
    20221 ataaacggcc agaaaaactc ccacaccgag acatcaaaac tgcacggcgt tttttgggca
    20281 acgacatctt cgcctgtaag cgggtagtga ttttgcatcc ataacaggcg gttaacgata
    20341 gccgtctgcc cgaccattac ccctttcggc ctgccggtgg agccagaggt aaagatgata
    20401 taagccgtgt ggtgcggttg tgaaagttgc agcggcgcac tgccctgggg ggtaagcggg
    20461 gcgttatagc aaaggcttgt taaattggga acatcgctaa agcgcggcag ttgatcgtcg
    20521 gtggtaatta acagcgacgg acgcgcatct tccagcatca ttttcaggcg atcgtccgga
    20581 tagccggtat ccagcggtag ccaggccgcg ccagcttcaa ctattgcatg gagtgccagg
    20641 gtcaaaaaga ccgagcgcgg tagtgccacc gccacgctgt cccctggttt aacgccgcgc
    20701 tcacgcagca gattcgccag cgccaccacc tgctcgcgca tttcccgata gctgaacagg
    20761 taacgcgcat ctgccagcgc cggagcatcc ggtgtttttg ccgcttgttc tgccaccagc
    20821 gcgctcagcg tggtttctgg aatctcaacc tgagtggcgt tgatctgcgc cagctgcgca
    20881 tactcacctg gcagcataat atcgacatcg ccgcacaaca gcgccggatc cgcggcgaac
    20941 tgggcaatca gcattttcag gcgttcagca tgctggatta acgttggctc atcgtaacgc
    21001 tgtttattgg cgaggatctc aatactcaaa tcaccgtgtt catccgggaa cagggccagt
    21061 tcaaggtcat taaccggacc ggttgccagg gtatgggttt gcgtctgaac accaggaata
    21121 tccagttggt aatcaaatac cttgatattg agtaccggac cgaacagcgg ttcctctcct
    21181 gccgctcgcc cgctgtcacg gacaatttgt tcggcatcgt aacgttgatg acgacgcatt
    21241 tttttcagtt gtgctgccag tcgggttgcc agttccggca gcgtttcttg cgccgcaatg
    21301 tgaataccca acggtaaaac gttgagcacg ggtccgctag ccgtcagcgc cgccgagccc
    21361 agtcgacgca taaagataaa tccggcggca tagtccatgc gattgcacaa tcgccccagc
    21421 cacaaggctg ccagcgcaag ggctaaatcg gtacgctgca cacctgaaag ttgcgtagcc
    21481 agctggcgga attccccgtc ggtaaattcc agtttcaggc gcagaatatc tgccgaagcg
    21541 ctgcgccccg ctaaaggtgc cggagaaagt gacgcgggcg gcggcagttg acgacgctgt
    21601 tctgcccaga atgccgcatc acgctgccag gcttcgcttt cgcggtattg ctggtactct
    21661 tccactacat cagcgaaagg cgtaaacggc gaagcaggcg ttggttcgcc acgcagccat
    21721 gtgcagtaaa tattggcgat ctggcgagta atggccggga aactgaagcc atcgaccagc
    21781 aaatggtgat aacgctgata ccagtaccag cggttatccg caacatgtat cagctgatga
    21841 aagaccagtg gtttaccgct atcgacgcgc aaatcttgtt gcaaatccgc cagcattaat
    21901 gcctgcgcag taccgtgcgg atcaatattg gttcgcaggt cgataatttc tggcagttcg
    21961 aatatcagcg catcatcgac ccactgccag acttcgccgt tatcttccgt aaaacgcatc
    22021 cgcaacgtat ctgcttgcgc tagtccgaca accaccgcgc gggccagtaa tggcgcatca
    22081 acatctccag ttaactcaac gtaatgcgcc acgctccagg cggagggtaa atccgacagt
    22141 ttttctgcca tccagatgcc gggctgtgcg gcgaccaaag gtaaatgctg gctcattgtg
    22201 cctcctgcaa ctgggtgaaa ttggccggtg tcagagtacg ccagtggact tccagccact
    22261 gctggcagga cgcctgtgac tgcggctgac acacaatgtc ccagcctgcc ggtaaggcgc
    22321 attgttgcgg ccacagactg aattgcccct gcgcattgcg caaaatgtaa aacgctccct
    22381 gtggatcatc gaagggatta ctgaatgcca tattcaactc ctgtcatgga aaagtggttg
    22441 ccagaggtcg attagcccct gcatcaagcc accgcgccaa caaagcgcat catgtccgcc
    22501 gtcaacctga cgccagaaaa tggattcttt tatggggtgt aattgcgcat acagcgcctg
    22561 attggcccgc ataatcatcg gctcgcgaat acccgcttcc agcacaatgc gcagaccttc
    22621 ggcgctaact tcaccagctt taagcttttc aagtaacacg ccctcttgct gcccgccccg
    22681 atgcggccac cagtacgatc ctgactggct taatacacag ccaaagcgtt caggccagtg
    22741 cagtccggca tacagcgcgg acagcccacc aaaactctgc ccggcgacca cggtgcgatc
    22801 ggcgcgatcg ctaaaagggg caatagcttt caccaggggt aataactctt gctgtactgc
    22861 cagccagaaa tccgcattac acggcagttc gtgggcgcgg tgcgtggtgt cgatagcgtc
    22921 gatcaacaca tacacggcgg gaggaagttg ctgacgatgg gtcagcgaag tcagcacagg
    22981 ccagacgggc atactttgcg cccaaaattc gccatcgagc aaaactgcca gcggacgttc
    23041 ttctgctgtt gcatcgccgg tggtaaaaat ccatacacga cgtgaatttt tcaaccgttc
    23101 acttttccag ataatttctt tggctgctat ttctggcgct tgcggacaat cccatcccgg
    23161 ttgcagaggc gcttgcggca tttcgagtgc agaaacagcg tgccctcgcc cgcctttcca
    23221 gctttgtgga ttcagcggat cggctattgc ctggggtaat agttttcgcc agccttcacg
    23281 taattcgagg cgatcggggg atggtgcaga aaaaatgtca tcgcgttcgg tgggaataaa
    23341 gcagtagctg ccgcgccagt tggcattgag ttgtgtcgtc cactgccaga catcagtacc
    23401 tgcaattcgc tgcatcgact ggggctggct gttctgatga tgatcggtca caccagtgat
    23461 gtagatccat acgcgcttta tcgtcgagta ttcttcagaa ccttggggat cacgccacca
    23521 gaaagtgacc tcaaacattt cgtcattcag acgctgccat tccgggccat gtttcgactg
    23581 ccaccagctc tcacttccta cttttaacgc cgtcacacca taaccccatg tttactgtgc
    23641 aatttttcat tgattgcaga aatatattga taatattatt gataactatt tgcatttgca
    23701 atagcgtaat ggcgcgccgt gggaagcacg gacatttatt aaccaactgc actgcgtgtc
    23761 tttcaggatc aaaggttttc gcggtagcgg gatgcgtcgt gttgaggacg accatgcccg
    23821 acagttgcaa ttcgtggcaa aaatgcagga ataaaacaat gaacaagaag attcattccc
    23881 tggccttgtt ggtcaatctg gggatttatg gggtagcgca ggcacaagag ccgaccgata
    23941 ctcctgtttc acatgacgat accattgtcg ttaccgccgc cgagcagaac ttacaggcgc
    24001 ctggcgtttc gaccatcacc gcagatgaaa tccgcaaaaa ccccgttgcc cgcgatgtgt
    24061 cggagatcat ccgtaccatg ccaggcgtta acctgaccgg taactccacc agtggtcagc
    24121 gtgggaataa ccgacagatt gatattcgcg gtatgggtcc ggaaaacacg ctgattttga
    24181 ttgacggcaa gccggtaagc agccgtaact cggtgcgtca gggctggcgt ggcgagcgcg
    24241 atacccgtgg tgatacctcc tgggtgccac ctgaaatgat tgaacgtatt gaagttctgc
    24301 gtggtccggc agctgcgcgt tatggcaacg gcgcggcggg cggcgtggtt aacatcatta
    24361 ccaaaaaagg cagcggtgag tggcacggct cctgggacgc ttatttcaat gcaccagaac
    24421 ataaagagga aggtgccact aaacgcacca acttcagcct gaccggtccg ctgggcgacg
    24481 aattcagctt ccgcttgtat ggcaacctcg acaaaaccca ggctgacgcg tgggatatca
    24541 accagggcca tcagtccgcg cgtgccggaa cgtatgccac gacgttacca gccgggcgcg
    24601 aaggggtgat caataaagat attaacggcg tggtgcgctg ggacttcgca ccattgcaat
    24661 cgctggaact ggaagcgggt tacagccgcc agggtaacct gtatgcgggc gatacccaga
    24721 acaccaactc tgacgcttac actcgctcga aatatggcga tgaaaccaac cgcctgtatc
    24781 gccagaacta cgcgctgacc tggaacggta gctgggataa cggcgtgacc accagcaact
    24841 gggtgcagta tgaacacacc cgtaactcgc gtattccgga aggtctggcg ggcggtaccg
    24901 aagggaaatt taacgaaaaa gcgacacagg atttcgtcga tatcgatctc gatgacgtga
    24961 tgctgcacag cgaagttaac ctgccgattg atttcctcgt taaccagacg ctgacgctgg
    25021 gtacggagtg gaatcagcaa cggatgaagg acttgagttc caatacccag gcgctgaccg
    25081 gggcgaacac cggcggcgct attgatggtg tgagtgccac cgaccgtagc ccgtattcaa
    25141 aagcagaaat tttctcgctg tttgccgaaa acaacatgga gctgactgac agcaccatcg
    25201 taacgccggg gctgcgtttc gatcatcaca gtattgtcgg caataactgg agcccggcgc
    25261 tgaacatatc gcaaggttta ggcgatgact tcacgctcaa aatgggcatt gcccgtgctt
    25321 ataaagcacc gagcctgtac cagactaacc cgaactacat tctctacagt aaaggtcagg
    25381 gctgctatgc cagcgcgggc ggctgctatc tgcaaggtaa cgatgacctg aaagcagaaa
    25441 ccagcatcaa caaagagatt ggtctggagt tcaaacgcga cggctggctg gcgggcgtca
    25501 cctggttccg taacgattat cgcaataaga ttgaagcggg ctatgtggct gtagggcaaa
    25561 acgcagtcgg caccgatctc tatcagtggg ataacgtgcc gaaagcagtg gttgaaggtc
    25621 tggaaggatc gttaaacgta ccagttagcg aaacggtgat gtggaccaat aacatcactt
    25681 atatgttgaa gagtgaaaac aaaaccacgg gcgaccgttt gtcgatcatc ccggagtaca
    25741 cgttgaactc aacgctgagc tggcaggcgc gggaagattt gtcgatgcaa acgaccttca
    25801 cctggtacgg taagcagcag ccgaaaaaat acaactacaa aggtcagcca gcggttggcc
    25861 cggaaaccaa agaaatcagt ccttacagca ttgtgggtct gagcgcgacc tgggatgtga
    25921 cgaagaatgt cagtctgacc ggcggcgtgg acaacctgtt cgacaaacgt ttgtggcgtg
    25981 cgggtaatgc ccagaccacg ggcgatctgg caggggccaa ctatatcgcg ggtgccgggg
    26041 cgtataccta taacgagccg ggacgtacgt ggtacatgag cgtaaacact cacttctgat
    26101 gctaacgtca gattgttgac aaagtgcgcg tcgttcatgc cggatgcggc gtgaacgcct
    26161 tatccggccc acaaaaatct tgccaattca atatattgca ggaccatgta ggcctgataa
    26221 gcgtagcgca tcaggcagtt tagcgtttgt catcagtctc taatatggtc gacatgaaaa
    26281 ctacgcatac ctccctcccc tttgccggac atacgctgca ttttgttgag ttcgatccgg
    26341 cgaatttttg tgagcaggat ttactctggc tgccgcatta cgcgcaactg caacacgctg
    26401 gacggaagcg caaagccgaa catttagccg ggcggatcgc tgcaatttat gcgctgcggg
    26461 aatatggcta taaatgtgtg cccgcaatcg gcgagctacg ccaaccggtg tggcctgcgg
    26521 aggtatacgg cagtattagc cactgcgggg ctacggcatt ggccgtggtt tcccgtcaac
    26581 cgattggcgt tgatatcgaa gaaatttttt ctgcgcaaac agcgaccgaa ttgacagaca
    26641 acattattac accagcagaa cacgagcgac tcgcagactg cggtttagcc ttttctctgg
    26701 cgctgacact ggcattttcc gccaaagaga gcgcatttaa ggcaagtgag atccaaactg
    26761 atgcaggttt tctggactat cagataatta gctggaataa acagcaggtc atcattcatc
    26821 gtgagaatga gatgtttgct gtgcactggc agataaaaga aaaaatagtc ataactctgt
    26881 gccaacacga ttaattgaca acatctggta cgattcgccc gcagccatca ctgaccgcgg
    26941 gcgaaagtgt aaagcaggtg ccttaccact ctgacctgac aaccggatat gcggggattg
    27001 ctccccgcac aacggctact tcttcggttc gtaagcgaga acagccttaa actcaatatt
    27061 acgttccttt acggtgaact cacacagcga gtctccgacc agaagcgtaa atcccagtac
    27121 cgtcaaacac agcactatga ctgccacaag ggcatatttc gtcagcatgt ttatattgcc
    27181 tccatgcgaa gaagggacta ccatccgagt tgttgaggct cggagtggca gccccaggat
    27241 tgatagaaat atctcctggg gcttcatctt tccagacctc agagttaacc tgaaaccgga
    27301 aagcttcagg cacccgccgt tatcttaccc ttcctcaaca aaatagctgt ttatttatac
    27361 agtgaatcct gtttcggact tctgtcggca aaactcatca ttgctcttaa aacgaaatcg
    27421 cccgcagcca tcactgaccg cgggcgaaag tgtaaagcag gtgccttacc atcctgacct
    27481 gacaaccgga tatgcgggga ttgctccccg cacaacggct acttcttcgg ttcgtaagcg
    27541 agaacaacct taaactcaat attacgttcc tttacggtga actcacacag cgaatccccg
    27601 accagaagcg taaatcccag caccgttaaa cacagcacta tgactgccgc aagggcatat
    27661 ttcgtcagca tgtttatatt gcctccatgc gaagaaggga ctaccatcca aattgttcag
    27721 gtttgggtag gtagccccag ggttgataga aatatcgtcc tggggcttca tctttccaga
    27781 cctcagagta aacctgaaac cggaaagctt caggcacccg cagttatctt acccttgctt
    27841 agcaaaatag ctgtttattt atacagtaag tcctgtttcg gacttctcac tccccatttg
    27901 atcaactgaa gctgaacccg tttttttctg catgcctcaa tcgtcaaaat tcttcgccac
    27961 cagccagccg tgccataact tgtcacattg caaaagtagc caaattgctt cagtcaccat
    28021 caccaaaaaa agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata
    28081 ctgaatagaa aacgccaact taagagaaac ctatgccatt acccgatttt catgtttctg
    28141 aaccttttac cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact
    28201 taagccagga ctcttcaatg ctgattgacg cggttaaaaa tgagatcacg gccggagagg
    28261 taaagcacga tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca
    28321 accaggctgc cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc
    28381 atcatctgga aatttgcggc ggtggcacgc acccgtttca gaaatggcag cgtcaggagg
    28441 tatgcgaaaa cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg
    28501 cgaccgtttt tggtcagcat gtccatgttg gctgcgccag tggcgatgac gccatttatt
    28561 tgctgcacgg cctgtcacgg tttgtgccgc acttcatcgc cctttccgcc gcgtcgccgt
    28621 atatgcaggg aacggatacg cgttttgcct cctcacgacc gaatattttt tccgcctttc
    28681 ccgataatgg cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct
    28741 gcctgagtta caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca
    28801 gtcctcattt tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg
    28861 cggtaaatat ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt
    28921 ttaaacatca ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgccgct
    28981 atgggctgga aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag
    29041 ataccttgcg attgctggaa aaaatcgccc cttccgcaca taaaatgggt gcatcgagcg
    29101 cgattgaggc cctgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg
    29161 atttcgtcgc cgatggtggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg
    29221 ccggtgacta agtagggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact
    29281 tacatttaac aatgttatga aacacccttt agaaaccttg actaccgcag caggcatttt
    29341 gctgatggct ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc
    29401 acaaaaactg gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt
    29461 ttgtctgtgg tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg
    29521 acgctggttc tcgctggcgg attaaacgtc gattatcgcc gtacttcgcg gcatactttg
    29581 cttattctcc ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga
    29641 aacgcctggc ccttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg
    29701 atgtttttaa aattggcggc aaaattttta tgctgtcgtc ggagctgcgc ggcgtcccct
    29761 ttatcaatct gaagtccgat ccacaaaaat ccctgttaaa tcagcagata tacccgagca
    29821 ttaagccagg gtatcacatg aataaaaagc actggatttc ggtgtatccc ggcgaggaaa
    29881 tctccgaagc gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc
    29941 tggctaaacg cgatcaaaaa agagtgcgtc caggctaaag cggaaatcta tagcgcattt
    30001 ttctcgctta ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttactttcac
    30061 atggagtctt tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg
    30121 atgcca
//
LOCUS       DAEVZA010000048        29829 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEVZA010000048 DAEVZA010000000
VERSION     DAEVZA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.048"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1348..2355
                     /locus_tag="LAF92_002226"
     CDS             1348..2355
                     /locus_tag="LAF92_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI9791228.1"
                     /translation="MVLKNNKNSDCNDVQSLLAQGNQLLEGAYDITLIEMRLLYLALT
                     KIDSRKPQPANEYTLFAKEYRDTFSLDSKNCYEQLKSAASSLGSKPIVTYEWNEAKKR
                     IDAVKRFWFSSIRYGVGNSESDITLRFSDSVSQYLYELKSEFTQMNLEHMVKLDTPFS
                     FRLYSWLYKYKNLNRNKKHSGVISTDPITIEWMKERTGLTGKYPVYKDFKKRVLDPAV
                     DIINANTNLSVTYEGIKSGRKIESVVFTYLVENETHDGKKIATKPLRPRLPSRPRVIK
                     GSSAEVAWAKKCIDVMKNYLISLREYDDKLKLSRPDLQKVKLWHEITGWDFEESFWFS
                     D"
     gene            2421..2825
                     /locus_tag="LAF92_002227"
     CDS             2421..2825
                     /locus_tag="LAF92_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791229.1"
                     /translation="MKDNIVDFQRIRRDLGAHSTDVANDAVYSDKKKTSRFFYLLTPV
                     RYLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSGYVVLAAWIA
                     LTVCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
     gene            2961..3488
                     /locus_tag="LAF92_002228"
     CDS             2961..3488
                     /locus_tag="LAF92_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBI9791230.1"
                     /translation="MIFNRNKKRDEQQNFPIVLPSDENVFSDFIKNNPALTTNAFTAI
                     GEVDCFTKVPLLSNKESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIRE
                     VQNITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDM
                     MGSEDFCQFLRDDEE"
     gene            complement(3517..3723)
                     /locus_tag="LAF92_002229"
     CDS             complement(3517..3723)
                     /locus_tag="LAF92_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791231.1"
                     /translation="MLFEPKTKCKYQAKAIKDEKSVERWRFVIISRDCNGIVQKGDEI
                     KYKSVLPDNNTENFIASGSLFTIY"
     gene            complement(3930..4148)
                     /locus_tag="LAF92_002230"
                     /pseudo
     CDS             complement(3930..4148)
                     /locus_tag="LAF92_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928750.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4323..4592)
                     /locus_tag="LAF92_002231"
     CDS             complement(4323..4592)
                     /locus_tag="LAF92_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791232.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNEL
                     LEFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
     gene            complement(4667..5314)
                     /locus_tag="LAF92_002232"
     CDS             complement(4667..5314)
                     /locus_tag="LAF92_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HBI9791233.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTK
                     KWNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADI
                     VIVPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLN
                     SHDYWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
     gene            complement(5727..5906)
                     /locus_tag="LAF92_002233"
     CDS             complement(5727..5906)
                     /locus_tag="LAF92_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791234.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFPGIDDRLSGMLMSVAVGCL
                     SWYVTRPKNQQTKAA"
     gene            complement(6817..7281)
                     /locus_tag="LAF92_002234"
     CDS             complement(6817..7281)
                     /locus_tag="LAF92_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HBI9791235.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNV
                     VIEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGG
                     VRKAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
     gene            complement(7297..7503)
                     /gene="hha"
                     /locus_tag="LAF92_002235"
     CDS             complement(7297..7503)
                     /gene="hha"
                     /locus_tag="LAF92_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBI9791236.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAAD
                     HRRAELVMNKLYDKVPSGVWKYVH"
     gene            complement(7500..9653)
                     /locus_tag="LAF92_002236"
     CDS             complement(7500..9653)
                     /locus_tag="LAF92_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBI9791237.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHI
                     IESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKAL
                     ARSVADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFY
                     YTMTGVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTVASLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQVIDALKSVFPQSLDIDPARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFCARARKTTDSGFEAFLGAETTDEGE
                     SEDNDDSAFELLCKIRTGETLTTKEVVVNEKKTTPPPLFTEASLLAALVRVADFVTDP
                     TIKKLLKDKDKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDA
                     LPGIAVNPDMTALWSEKQTAIENGDLTVEEFINELYGELTGMISDVDLGEMKIEPAAP
                     AGQFQRLDSPCPSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTD
                     LIKGFKKKNGGTYDTVLVLEDKKTGKLGFPARAKK"
     gene            complement(9658..10233)
                     /locus_tag="LAF92_002237"
     CDS             complement(9658..10233)
                     /locus_tag="LAF92_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBI9791238.1"
                     /translation="MQYALVDALERKFLLDALEFGVLKDWKENPVKELPDIDESVHPF
                     HVCYGGYLLNPGVSDSDISKKIKDQTGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGH
                     QKIVPFAALIKADECIISKIASYSGFAVTAFLRIKDQDIATNILNREGIFAFNGCERR
                     FRQPVSEDNWQQAVSEERAIRCANRLIQCKG"
     gene            complement(10237..11499)
                     /locus_tag="LAF92_002238"
     CDS             complement(10237..11499)
                     /locus_tag="LAF92_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9791239.1"
                     /translation="MGKLTLVEQALRLHKEYYGEDNFGTVVKDCDYYPKVAEIFINRK
                     NRKIYSNLDIEPVASTVVHTGSNPVMAKAGRIKKLMFKDGSGAYRIHLGQNEVVHIIR
                     FILNSKVRMEYAVGTTKSMSMLHNLCEAGRAKLNRSLPRKGIWKLGYYDGVYYHGKAR
                     KEEIENAMRFSVHPKFDELENDFNQFFSDIDFYTRYGQSGMRKVLFTGPPGTGKTTIA
                     KALGAKYQDKYVFVYADDYFKDVCYAAAQKKIPVIIIAEEVDELYRADAGTLSFLDGA
                     DTPRNVAGTYVIFSTNYPKRIDPRIRKRPGRIDRIISVGAFRTKAAAACAKMYLPEDV
                     EIDLKELGAVLDRTTPAEIKEIINIAIGMIRGTKNELSVDVIKNARAFLKGTLDLSVQ
                     EADEDIEEREELYKKNGAQPDYLSYLED"
     gene            complement(11581..12075)
                     /locus_tag="LAF92_002239"
     CDS             complement(11581..12075)
                     /locus_tag="LAF92_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBI9791240.1"
                     /translation="MRHFLKALSFVVSLLPATLCAAEIQGKVIRVLDGDTIEVKTLPA
                     KIVVYEVPIRVRLINIDAPEKKQAFGHWSANQLKALLAGQSVTVSYTQTDRYGRIIGR
                     VFTTNGTDASRFMVQSGAAWVYERYNTDKSLPALQREAQEQKRGLWADSNPVPPWEWR
                     HKQN"
     gene            complement(12072..12380)
                     /locus_tag="LAF92_002240"
     CDS             complement(12072..12380)
                     /locus_tag="LAF92_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791241.1"
                     /translation="MKARSVRVAGIEIIDSVKTVAYVFTEALLFMVWFSAIVYGLYRV
                     VIVLWDDPLKPQSLMEFLNFIVELYSYVLPSLAVIAVALIAVLSICKILSAIWEGRKQ
                     "
     gene            complement(12472..12720)
                     /locus_tag="LAF92_002241"
     CDS             complement(12472..12720)
                     /locus_tag="LAF92_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791242.1"
                     /translation="MSEIRFVTLESDFNNIKEIYSDDELNRLLEAVNQNCPTRIVLGS
                     VEKGKETPLKWCFRTIPERISIEEIDEIKFIQNPSEQG"
     gene            complement(12775..13080)
                     /locus_tag="LAF92_002242"
     CDS             complement(12775..13080)
                     /locus_tag="LAF92_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBI9791243.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGG
                     SECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVKG"
     gene            complement(13064..13483)
                     /locus_tag="LAF92_002243"
     CDS             complement(13064..13483)
                     /locus_tag="LAF92_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="HBI9791244.1"
                     /translation="MKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKM
                     EGATAVVEWRNEKSFLPEQGKIVFIKAQNEVKNDWKK"
     gene            complement(13495..15357)
                     /locus_tag="LAF92_002244"
     CDS             complement(13495..15357)
                     /locus_tag="LAF92_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBI9791245.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKA
                     VFRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKDN
                     IFGDARFATTNDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKG
                     AGIVITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSPVTHRFNPLYY
                     VSMGTSEGFNQLENLALIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAG
                     LKTLDIEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIE
                     MEDKTKAQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLA
                     EKITNIFFQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKL
                     KLLIVYQVGSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGS
                     KSKSRSRGGTSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLHE
                     YFFGDFVKVAPELANIFPRKKGKLVMPPQKVFENEIVAKGYLAVKEVPDLDKAA"
     gene            complement(15344..16372)
                     /gene="virB11"
                     /locus_tag="LAF92_002245"
     CDS             complement(15344..16372)
                     /gene="virB11"
                     /locus_tag="LAF92_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HBI9791246.1"
                     /translation="MNGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWT
                     EINGDWTFHNNDGVTFDFCKRFSQTLASFRGDEIGDTKPLLSATLESGERVQVVFPPA
                     CERNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFM
                     ELAVAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLF
                     YPSESGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSI
                     HAASAKDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYY
                     KGAECENF"
     gene            complement(16374..17492)
                     /locus_tag="LAF92_002246"
     CDS             complement(16374..17492)
                     /locus_tag="LAF92_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HBI9791247.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFG
                     LMAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNYDPVENKAII
                     NPGATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERK
                     ESKENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSA
                     NHHTKLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEA
                     GIDGWIDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVA
                     LENSINIPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
     gene            complement(17485..18393)
                     /gene="virB9"
                     /locus_tag="LAF92_002247"
     CDS             complement(17485..18393)
                     /gene="virB9"
                     /locus_tag="LAF92_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HBI9791248.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTV
                     IRSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKL
                     KKVFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEE
                     VRLAKEKQEKLRQKKLISESFEKADAPKNWDYFMRVNEKYDSRRIAPDFAYDNGIFTF
                     LGFNSGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVN
                     KSFGKVLTDQRNTSSPAVERVEVNND"
     gene            complement(18390..19076)
                     /locus_tag="LAF92_002248"
     CDS             complement(18390..19076)
                     /locus_tag="LAF92_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBI9791249.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCL
                     GAAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREG
                     YNYPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDA
                     TAPDKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTS
                     YQTDREVRGE"
     gene            complement(19591..20685)
                     /locus_tag="LAF92_002249"
     CDS             complement(19591..20685)
                     /locus_tag="LAF92_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBI9791250.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYV
                     LWKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGV
                     SVWQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTA
                     DVTMKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMI
                     LSHAIRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMM
                     GLGAAGMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEK
                     GYQALAPTGALGMKARRLASLEKARARNAA"
     gene            complement(20697..21413)
                     /locus_tag="LAF92_002250"
     CDS             complement(20697..21413)
                     /locus_tag="LAF92_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB5"
                     /protein_id="HBI9791251.1"
                     /translation="MKRVKTFMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERL
                     KQWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDF
                     TSNPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLS
                     NAMSQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLI
                     TQEQEAFIKQQKEAEPLTFD"
     gene            complement(21425..24172)
                     /locus_tag="LAF92_002251"
     CDS             complement(21425..24172)
                     /locus_tag="LAF92_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HBI9791252.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAI
                     PVAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAIEIDEFVNA
                     MKLNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVA
                     TLVRSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYF
                     TLIYRPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEE
                     NGKVFSSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFR
                     SIEIKDFCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDA
                     VTQLQELEKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPAL
                     STMSLPAAYFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILK
                     TPSKQAYYLNLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPET
                     FADSAKNKKLTCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFV
                     KQLMEILCTRNGERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDE
                     QENGIILRLSQWANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYL
                     LYRITQLLDGRRLVIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEIL
                     KNKISRAVVEVCSTSIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSS
                     LKKGDGKSFSALATLDLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERA
                     I"
     gene            complement(24191..24463)
                     /locus_tag="LAF92_002252"
     CDS             complement(24191..24463)
                     /locus_tag="LAF92_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HBI9791253.1"
                     /translation="MQRKNKVVMAVAGATVASPAFADGFSKAETLLEKVKTGLSGLSL
                     VTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVASMMVN"
     gene            complement(24531..25151)
                     /locus_tag="LAF92_002253"
     CDS             complement(24531..25151)
                     /locus_tag="LAF92_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBI9791254.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERER
                     GSYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITNSNFSFYSTSAEKLLDPC
                     ENLSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEK
                     KYVVPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
     gene            complement(25154..25330)
                     /locus_tag="LAF92_002254"
     CDS             complement(25154..25330)
                     /locus_tag="LAF92_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791255.1"
                     /translation="MKEKPDKSSEKGVYVTIKLDPRLNAFIEEKTKTSLLSKRKQIIY
                     LLMQSMRIDGYMER"
     gene            complement(25507..25722)
                     /locus_tag="LAF92_002255"
     CDS             complement(25507..25722)
                     /locus_tag="LAF92_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HBI9791256.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSG
                     SDSQNCKNVKQALFIQSQKDAPVPTFD"
     gene            complement(25719..26012)
                     /locus_tag="LAF92_002256"
     CDS             complement(25719..26012)
                     /locus_tag="LAF92_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBI9791257.1"
                     /translation="MTEHDAICISALHQIFSDEEHLSEQQKDIILMYAYGYTLNEIAD
                     FKGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
     gene            complement(26089..27318)
                     /locus_tag="LAF92_002257"
     CDS             complement(26089..27318)
                     /locus_tag="LAF92_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBI9791258.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRH
                     GTDKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKG
                     EDFNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATIEFLKET
                     YPNHAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGY
                     DVTATWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETL
                     KGGKQVTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRN
                     NWQIENIALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEE
                     HKKLRIQQERNWKGLGF"
     gene            complement(27322..27825)
                     /locus_tag="LAF92_002258"
     CDS             complement(27322..27825)
                     /locus_tag="LAF92_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791259.1"
                     /translation="MKNVCFRLNDDEYELARVNAASYGVDSVNVFAKMKVLDVPTKPV
                     SIPVKNEAAKPVFTYLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKNEVCLYPDE
                     VKELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTK
                     SVVNRFG"
     gene            complement(28186..28533)
                     /locus_tag="LAF92_002259"
     CDS             complement(28186..28533)
                     /locus_tag="LAF92_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBI9791260.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELE
                     QEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNK
                     ALEKEVSNHRAGA"
     gene            complement(28530..28778)
                     /locus_tag="LAF92_002260"
     CDS             complement(28530..28778)
                     /locus_tag="LAF92_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBI9791261.1"
                     /translation="MGKTDKLQAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRF
                     FNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
     gene            complement(29083..29433)
                     /locus_tag="LAF92_002261"
     CDS             complement(29083..29433)
                     /locus_tag="LAF92_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791262.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCT
                     FLFDPKRTLAELDKLIKKIEVEEDYEFCVSLDKGNGLHEVRTFKSEREALDAYRILTL
                     YGYTVTIEKKEGND"
BASE COUNT         8438 a         6677 c         5537 g         9177 t
ORIGIN      
        1 tggtgcattg cagcatggcg gggcgctgta ctgcgcgtgt gagcgtcagc gagctacggg
       61 cgcaatgcag gagaactaac agaacctccc taaaacgctc tgaatcgcat tttaagcgat
      121 ttttttcatt tcgcatggtt ttcctcatgg ttgtatcgaa cgaagcggca gagcgcgatt
      181 cagggcttct ctgtgacact ttggggaggg ggcattgctg aatctgttga tttatgtatc
      241 gttgaaatga tgtggtcaca taaatcaatt ggcaaaagaa tatatcatgt tgatattaaa
      301 ggtatttaat tgaaacatga gaagatcctc ggttagcaaa atgaggatca taattaaacg
      361 aactattcca ataaaatcag ttagttatat taaaccgccc tcggaatgaa atattcaggt
      421 tgtgactaaa catgagaact ttctcggtct tcacaggatc agattctgat gaatagaaag
      481 cctctgtaag cgtttttcgc gcctcccatg ccctaagggg tatttaaacc cgtttcgtgc
      541 gtctgagagc ttctgagcgt gttttatagg ctatcaagcg acccatcacc gaaggaacaa
      601 caacagaatt accagcgata tgtgagaaat gactccgctc gccgcagtcg aacgaccgag
      661 cggagcgagt tagtgagcga ggaagcggaa tgtcttcgat acagcatatg aatccgtacg
      721 ctctggtctg acctcatctt gtgattttgc tgattccaac tcaaaaaatt cacaatgtgc
      781 tctattcttt ttttctaatt tcttttttct aaaagataga tcactcctat gttacatata
      841 atatttcctt tttcaggggt gttttttctt ttgtttttca attgattgca atgctaacta
      901 gggacagatt tatggttaaa aggggacaga tttatggtta tttgcgtttt tatcagggga
      961 cagatttatg gttaaaaggg gacagattta tggtcaacta gggacagatt tagtgtggat
     1021 aactttttta tccacaggtt gtggataact ttttttagat tcaactcgga ttcgttatgg
     1081 attctctatg aatctgaatt taattagtga cttagatcac atttaagatg tttggctcta
     1141 tgggtttttg atgatgcatt ccctcctggt ttgctgtatt gactgcctct gactgagggt
     1201 gaaaatgcgc cgttcagcca ggtttgaccc ttcaaacggg ggacagattt atggtttatt
     1261 agttatatag ggacaaaaaa aaattgcgtc cctagtttgg gtgtgagaaa cttcttttga
     1321 tttttaactc acgggaaggg gagctgtatg gtcttaaaga ataataaaaa tagcgactgt
     1381 aatgatgttc aaagtttgtt ggcacaggga aatcaattgc tggaaggtgc atacgatata
     1441 acgctaattg aaatgcgtct tctgtatctg gcattgacta agattgatag ccgtaaacca
     1501 cagcctgcaa atgaatatac attgtttgcg aaggaataca gggacacttt ttctttagat
     1561 tcgaaaaatt gttatgaaca gttaaaatct gctgcgagtt ctttaggctc aaagccaatt
     1621 gttacgtatg aatggaacga ggcaaaaaaa aggatcgatg cagtcaaacg attctggttt
     1681 tcttcaataa gatatggtgt tgggaatagt gaaagtgaca taactttgcg tttttcagat
     1741 tcggttagtc aatatcttta tgaactgaaa agcgaattta cacaaatgaa tcttgaacat
     1801 atggtgaaac tggatacgcc attttcattt cgactttatt catggttgta taaatataag
     1861 aatctgaaca gaaataagaa acatagtgga gtgatctcga cagatcctat aaccattgaa
     1921 tggatgaagg agcgaaccgg gcttacaggg aaatatccag tatataagga ttttaagaaa
     1981 cgggtgcttg atcctgctgt tgatattatc aatgcaaata cgaatctgag tgttacctat
     2041 gaaggtatca agtccggcag aaagatcgag tctgttgtgt tcacatatct tgtagagaat
     2101 gaaacacatg atggaaaaaa aatagcaact aaaccgttac gccctcgatt gccatcaaga
     2161 ccaagagtta ttaaaggaag ctctgcggag gtagcctggg caaaaaaatg tatcgatgtt
     2221 atgaaaaatt atcttatctc tttaagggaa tatgatgata agttaaaatt atcaaggcca
     2281 gatcttcaga aggttaagtt atggcatgaa ataaccggat gggatttcga agagtcgttc
     2341 tggtttagtg attaaagaat gctgctgttt acgttacccg ttttttctct ttttataact
     2401 cacttaaaag agaggatgat atgaaagata acatcgtgga ttttcaacgt atcaggagag
     2461 acctgggcgc tcattcaaca gatgttgcaa atgatgccgt ttattctgac aaaaagaaaa
     2521 catccaggtt cttttatctg ctgacacctg tcagatacct tctggctgct tccctttatc
     2581 tggttgttgt tattccattg gggattcttg cgggatttcg tttcttgttg cagtttttag
     2641 gcggtatcgc agtgatgggt ttaactatcg cttggtacat gggtgcagat gtcagtggtt
     2701 acgttgttct tgctgcatgg atcgccttaa ctgtttgtgg ttgcgcagag cagatttttg
     2761 aatggtggat cgaaagccgt tttgcgttcc gtctttttgg tatcgggaga acgccggaag
     2821 aatgagcgcc gatcttacag acagtaaaga acaatgcagg atagacaatc cactagaaat
     2881 tgcatgcaat tttcagtaga ttgtccaaaa acgatgtttt ttcctgtgcc tgccgcaggc
     2941 tcttttaaaa ggaatatgaa atgatattta accgtaataa aaaacgagat gaacaacaga
     3001 attttcctat tgtcttgcca tcagatgaaa atgtattctc tgattttatt aagaacaatc
     3061 cagctttgac aaccaatgct tttacagcca ttggggaggt tgattgtttt actaaagtac
     3121 ccttactctc aaataaggaa tcagagtttt taggcattct ttcacgtaat attatacctg
     3181 attattccgt ttttcctaaa atacggctca ttgaatttgt tcgtcctcat ggtagcgagg
     3241 aagctgtaaa agatttaatc cgtgaagtgc agaatataac ctgtgatttt gtcctaaaat
     3301 catatgatga aacagtcctg gctgttatcc agtttggtga aaccgcaacc gtcagacagc
     3361 aaaagaaaaa actgattatc cagcgcgtgt gtgctatgac aggaatttct tttttcgaat
     3421 ttaaaaacac tgtcgatatg atggggagcg aagacttttg tcagttcctg cgggatgatg
     3481 aagagtaaat caaaagccct ttcgggcttt tattttttaa taaattgtaa ataatgaacc
     3541 cgacgctata aaattttcgg tgttattatc tggtaaaacc gatttgtatt ttatttcatc
     3601 tcctttttgg acaatgccat tgcaatcacg acttattata acaaatctcc atcgttctac
     3661 cgatttttca tctttaatag cttttgcttg atatttgcat tttgttttag gctcaaatag
     3721 cattagatat tgtggttccg taccataaaa atcattcagg ctaatagcct ttgtttcaga
     3781 tgcaattgcg acagaggaca tcactatagt acatatgcca gtgatggatg tagttaacat
     3841 tttccgtata tgttcaactt tatttttcat attctcctca ttttagctga ctctatatat
     3901 ttttacataa aattaaccat agccatactt cacttcacaa aatcttctgg ttttatactt
     3961 gatttaaact cttcttcttt agaacgttct ttttgccatg ctatatacca tacaaacaaa
     4021 acaacagtgc caatgttact gtaacccacg agaaagaatc tgaaccggac gaccgcaacg
     4081 gttcagcata tatagatctt gccgccaagt aacataaaaa tgaaaaccgt tgttactggg
     4141 agcaacattg ccaggacaaa aacaatcaga ttacttttcc tgccaatatt aaatgctata
     4201 ccagccagta acactaaaaa gaaaaataca actggtacag tccatgtaat gacggcatca
     4261 tagttcatga tgcctaaatc aaagatgcat tgtttcatag agttaaactc ctccgtgaga
     4321 aattaatcat ttctgtcagc tctgaaccac aaattaatga gttcgttttc gtccagatcc
     4381 tgataagcca gtaatgctgc tttgattatt tttgtcttgg gacgatgcag tctttttgat
     4441 tcgttatcaa tgaattcaag caattcattc ggtatgcgga atgaagccag ttttgttttg
     4501 ttctctgtgg ggcgttttcc tgcgtcctga acaaattttg tggcgttcac ttcctggttt
     4561 tgttctagtt ctggtagttc cagctttgcc atgatgtacc ttctaatctg attgtttcac
     4621 actaatgtat atcatgatga tgtacattag tgtatgtcac tatgatttac agttccagtt
     4681 ctttcagcaa gagttcgatt tgtgcttttg cctggctggc accgctaaca ttacttttta
     4741 actcgtgaac ccccattccc tgattcattg ctcttacaaa ggcccggaga aaagaaaccg
     4801 tgcttttcat tggtttaagc cagtaatcat ggctgttgag agagtctctt aattcctgtt
     4861 gttcactgaa cgtatttggc tggcatttat acaggagcac atgtgcagcc agcctgggat
     4921 ttattttttg cgcatccctg acaatttttg tcacagtctg aagactgtct gcttccacct
     4981 gggcaagcgg gtcgatcggg acaatcacaa tgtctgcaac ttttagtgca gttctgaact
     5041 ccgtgctgtc atatccggca gtatctataa tgaggatttc tgctacatcc gccatttttg
     5101 ttattttggc actgatgtcg ccataggctt catttaatgc gacgggcggt aatccggcct
     5161 gttctctgcg tttattccat tttttggtcg agccattcag gtctgtttca acaagccccg
     5221 ttctctttcc ctgttgtgag aggcaaacag ttaagttggt tgccagatgt gatttacctg
     5281 taccgccttt atcaccaccg attacgataa tcatagtacc ccttgtatgt cacagtgatt
     5341 tacttatgtg tatttctatg tgatgtacgc atgcgtatgt ctcatggaca tactatagtg
     5401 tatgtcataa tgaattacaa taatgtacat catgaagaaa tacattattg tatatcagtg
     5461 tgatgtaccc gttggttggt cacttgaaag caaaagtgac agcgttactt tcggtattct
     5521 cccttgctgc gaaaccttgt ggcaatacag caatgagtat gtttagttac aacattcaaa
     5581 attaagcgat tagattccgc ccgtacgggc ggggcgcgcc ctttctgtag tggggagggg
     5641 cagggaggtt atgtttgctg gatgagcgga ggatggaatg aaccattaga atgttgtaac
     5701 taaaagatat cccaattaaa ggccacttac gcggcctttg tctgttggtt cttcggtctg
     5761 gtgacatacc acgataaaca tcccacagca acactcataa gcattccgga taagcgatca
     5821 tcgatgccag gaaacaggca accgacaaac atcgcaacag ggaaaggaag aagtaaacag
     5881 cgccccaaaa tgctcataaa atccataaat cctccttttt tgttaaaggg caggaagcgg
     5941 ttcacttcct gcccttagtt acgtttccgt tgtcatcaat cgatgaattg gcggatcagt
     6001 tccagaactg caatcagcgc gtttgcaacg gcgaccacag ctctgagaac gatgattaac
     6061 atattaatca tccgtttctc cagtaaaact ggaggaaatc accgttgctt gctcatacac
     6121 tgcctgtcac aacggctctc tcgcattgtt aggtgtagag tgacctcctc ctgccagagc
     6181 accgaataca gcaccacctg tttatccagc caggacttta aaatcgttca tacccgcaga
     6241 tacgggaaca gcaggattgc aagccaacta catgactaac tgactgatta tacgctacac
     6301 aaaacacccg ttacaccact aaatgtggtt ccttaaccac atttagttgc tttacagcac
     6361 catagtgaat aaaatgtaaa cgttaggtgt agagtaacct tctccggtca gagccaccaa
     6421 ctcaagactg gaaaacgcaa aaaagcccga ttaccagtcg ggtttttttg tgcctgtcgt
     6481 tctgctgtag ttccgttttt tctccttcat ttgagggatg tttctttcat tttttttgtt
     6541 gcttccaata ctgcctgtcg cgttggccaa cagcttgacg cgttaccggc attttcatta
     6601 ctcaatattt ttcagtgaat taaaatgcaa cagcgacaga aaaggacatt cagcttttta
     6661 gatgaatgct atagcaacgg tgtgctacgt aaaatctgcg aaaaaagcag gggtagggta
     6721 ggttattttt cacgtagcaa cgccatctgg cactgtacat acgtatgtca cacagacata
     6781 cctatgtgta tgtctgtgtg atgtatactt ggcgggttat tctgcctgtt ccggcttagt
     6841 tttctcaatg aggaaatctt ccatgaaatg cccctcatca agcagcttct gtaaagcaag
     6901 cggacgcttg ccattaccag accattctct gatctcgcca ttttcatcct taaagcggta
     6961 tttagccttt cttactccgc ctttggcttt ccgcctggtt tttactgtat cagctgttac
     7021 aggagcaagg agtgattcgg gatcaagacc taaagatatg atgtattcaa tggcctgctg
     7081 acgtttggct tcaagttcct ggcgttcagc cgcttcacgt tcagcatctt cacgacgttc
     7141 ttcaataaca acgttgagct tttcctgcat ttcgagcaac tgctcaaaat caatctcacg
     7201 ggcaaaaaca cgcaatgaac gaatattcat catagtccgg ctgataatgc cgtattcatc
     7261 ttctttagtc agttcgctca taattaatcc tcttgtttag tggacgtact tccagacgcc
     7321 ggaaggaacc ttatcataca gtttattcat caccagttct gcgcggcgat ggtctgcggc
     7381 agaataaaat gcctctgatt ctgataaagg cagcttgtaa cggttaatct caataacttt
     7441 ttcaagagta tctcttgatg tacattttct taactgaaaa agccattcct gctttgtttt
     7501 cacttcttag cccttgccgg aaaacccagc ttccctgttt tcttatcctc aagaacaaga
     7561 actgtatcat acgttccacc gtttttcttt ttaaatccct taatcaaatc ggttttccct
     7621 gatttaacca gtttttctgc ctgcgcctgg gtgattttct taccagaaaa ctcactccag
     7681 attttaaatt cacatccggt acagaaataa ccttttggtc tgataacaat atgtttacca
     7741 caggaagggc agggagagtc caggcgttga aactgccctg ctggcgcagc gggttcaatc
     7801 ttcatctcgc ccaggtcaac atcagaaatc atgcctgtca gttcaccgta cagctcatta
     7861 ataaactctt caaccgtcag gtcgccattt tcaatggctg tctgcttttc agaccataat
     7921 gctgtcatat caggattaac tgctatacca ggcagggcat caataagcgc atatccggta
     7981 tcagtaggaa taagtttccc tttttccagc gtgatatagt ttcttttctt cagtgtttcc
     8041 agaatggctg cgcgggtagc tggcgtacca atgccaccat gttcatcttt tttgtcttta
     8101 tccttatctt tcagcaattt cttaatcgtt gggtcagtga caaaatccgc gacgcgaaca
     8161 agcgcagcaa gcaaggaggc ttcggtaaat aatggcggcg gtgttgtttt cttctcatta
     8221 acgacaactt cttttgtcgt cagtgtttct cctgtgcgaa ttttacagag cagttcaaaa
     8281 gcggaatcat cattatcttc tgattcacct tcgtctgtgg tttccgcgcc aagaaacgcc
     8341 tcaaatccgc tgtcagttgt ttttctggca cgggcacaga acgactcatc accacactga
     8401 atggcaaccg atacttcctg gtatgctttt tcaggcatga actgaacaag atagtgttgt
     8461 gcgatcgcca ggtaaacatt gcgctcgtcg gtgctgagtg cgttaacatc aggcacactg
     8521 gcggtcggga ttattgcagt atgcgcagtc acctttgcac tgttaaacgc tttgctttta
     8581 cgtgcaggat caatatccag cgactgagga aagactgatt tcagggcatc gataacctgc
     8641 ggcgcttcgc tgaattgttc atcagaaaga tatgagcaat cagagcggtt ataagtaatc
     8701 gctttatatt tttcacgtaa ttgttgcgta atatccagtg ttttttgtgc cgtcattttg
     8761 aactttttgt tcatgtattg ctggagtctg accaggttaa acggcaacgg tgcagccgtt
     8821 tttttatcat cagttgctgc cgcttcaact gtagccggtt ttcctgcaag ggatgctacc
     8881 gttccgtctg cccacgcttt atcaagtaat ttacggtcgg tcagcggagc aaattcccct
     8941 ggcttccagt tcgccctgat aacatcagca ccacgctgaa aaactccggt catggtgtag
     9001 taaaaactgg atttatggtt ctggttagca cgggtacgat tcacaatcag gccaagaacg
     9061 ggagtctgga cgcgcccgac agacagaaca ccctgatatc ctctggcttt tgccggaatg
     9121 gtgtaagcac gcgtcatgga gaagccatag acggcatcag caactgaacg cgccagcgcc
     9181 ttaaggtaaa gccctttgaa atcacgatta tctttaagat ttgccagtgc ctttttcact
     9241 gccggaagcg tgttgtcgtt aatcagaacg cgctttacgg gttttgtgtt tcctgcatat
     9301 tccaggactt catcaacaag tagctgtccc tcatcatcag gatcgccagc gtgaacaatt
     9361 tcagtcacgt ctccacgtct gataagctcg agaatcgttt taacctgttt tgctgcactt
     9421 tcaacaggct gatacttcac gggataaaga cgtaaaggaa gcgtttcaac cttccaggct
     9481 ttgtattcag gattatagtt ttccggcggc tgtgattcga taatatgacc aaaacagtta
     9541 gtcacaatgg cgttatcact ttcaaaccag ccatcatggc gggtaaaatt gccaccaagt
     9601 gccttaacaa tatcatttgc tactgcgggt ttttctgcga taaaaagtct cattttgtta
     9661 tcctttacat tgaattaatc tgtttgcaca acgaatagcg cgttcttctg atactgcctg
     9721 ttgccagtta tcttcactta caggttgtct gaatctgcgc tcacagccat taaaggcaaa
     9781 aattccctca cggttaagta tattcgttgc gatatcctgg tctttaattc tcaaaaaggc
     9841 tgttacagca aaaccagaat atgaagcaat ttttgaaatg atgcattcat cagcttttat
     9901 taatgcagca aaaggaacta tcttttgatg tccacacgaa atcaaaggga gggtgtgaat
     9961 atcataataa gctattgaat gacaatccat gcgggtatca tcaatagctg ccagccagaa
    10021 tcctgtctgg tcttttattt ttttgctaat atctgaatct gaaacaccag gatttaataa
    10081 atatccacca tagcagacat ggaaggggtg aacagattca tcaatatcag gaagttcttt
    10141 taccggattt tctttccagt ccttcagaac accaaattca agagcatcca gcaaaaattt
    10201 tcgttccagc gcatcaacta atgcatattg catgtgttaa tcctccaggt aacttaaata
    10261 gtcaggttgc gccccgttct ttttataaag ctcttccctt tcctctatat cttcatctgc
    10321 ttcctgcacg ctcagatcaa gggttccttt caaaaaggca cgagcatttt ttattacatc
    10381 cactgataat tcatttttgg ttccgcgaat cattccgatt gcgatattaa taatttcctt
    10441 gatttcagcc ggagtcgttc tgtccagcac tgcgccaagc tctttaaggt caatttctac
    10501 atcttctggt aagtacattt tggcgcaagc ggcagcagct ttggttctga aagcacctac
    10561 agagattatc ctgtcaattc gacctggacg tttcctaata cgtgggtcga tgcgttttgg
    10621 atagtttgta gaaaagatca catatgtacc tgcgacattt cgcggtgtat cagctccatc
    10681 aagaaaactt aacgttccgg catcagcccg ataaagttca tcaacctctt cagctataat
    10741 aatgacgggt attttcttct gagcagcagc gtagcaaaca tctttaaagt aatcatcggc
    10801 ataaacaaaa acatacttat cctgatactt tgcccccaaa gcttttgcga tagttgtctt
    10861 gcctgttcca ggggggccag taaagagcac tttacgcata cctgactgac cataccgggt
    10921 atagaaatca atatcagaaa agaactgatt aaagtcgttt tccagctcgt caaattttgg
    10981 atgaactgag aaacgcatag cattttcaat ttcctccttt cttgccttac cgtgataata
    11041 aacaccatca tagtacccaa gtttccagat acctttacgt ggtaatgagc ggtttaattt
    11101 agcgcgtcct gcctcgcaaa gattgtggag cattgacata cttttagtag tcccaacggc
    11161 gtactccatc ctgactttag agttaagaat gaagcgaata atatgaacaa cttcattttg
    11221 accaagatga atcctgtatg caccagaacc atctttgaac ataagttttt taattctccc
    11281 agctttagcc ataacagggt tacttcccgt atgaacaacg gttgaagcaa caggttcgat
    11341 atccagattc gaataaattt tacggttctt tctgtttata aaaatctctg ccactttagg
    11401 atagtaatca caatccttta cgacagttcc aaaattatct tcaccgtaat attctttgtg
    11461 aagccgtaaa gcctgctcta ccagagttaa tttacccata agcgcaccat gtatatttaa
    11521 aaccgtgaca ctgcctgaaa tttatttaag gtagtggagg ggttggacaa gcaacgcgcg
    11581 tcagttctgt ttatgtcgcc attcccacgg tggaaccgga ttactgtcag cccataaacc
    11641 acgcttttgt tcctgcgctt cccgttgcaa agccggtaat gatttatctg tgttgtaacg
    11701 ttcatatacc catgcagcgc cagactgaac cataaagcgg ctggcatccg tgccgttcgt
    11761 cgtgaacaca cgaccgataa tgcgtccgta gcggtctgtc tgcgtataag agacggtaac
    11821 tgattgtcct gcgagcagag ctttaagctg atttgctgac cagtgcccga aagcctgttt
    11881 cttttcagga gcatcgatat ttatcaatcg aaccctgatc ggaacttcat acacaacaat
    11941 tttcgcgggt agtgttttaa cttctatggt gtcgccatcg agaacgcgaa taactttccc
    12001 ctggatttca gccgcacaca atgtagctgg taacagggaa acaacaaagg aaagagcctt
    12061 aagaaaatgt ctcattgctt tcttccctcc catattgctg agagtatttt acaaatggat
    12121 aaaacggcga ttaaagccac tgcgataaca gccaacgaag gaagtacata gctatacaac
    12181 tcaactatga aattaagaaa ttccatgagc gattgcggct ttaaaggatc atcccataaa
    12241 acgataacaa cacgataaag cccatagaca attgccgaaa accagaccat aaacagtaag
    12301 gcctcagtaa aaacataagc gaccgtttta acactatcga taatttcaat tccggcaact
    12361 ctcacacttc ttgctttcat atatacctca tggataacgg gaaactaacg cccttcggtt
    12421 gttcaacccc gcaaatccca gattaaaatc tggaatctgc ggcgtttacc tttatccctg
    12481 ttcagaagga ttctggataa attttatttc atcgatttcc tcaattgaaa tacgctctgg
    12541 tattgttcta aaacaccact tgagtggtgt ttctttcccc ttctccacgc tacccaacac
    12601 aattctggtc gggcaattct gattaacagc ttccagcaaa cggtttaact catcatcaga
    12661 ataaatttct ttaatattgt taaagtcgga ttccagagtt acaaatcgga tttcgctcat
    12721 aatttttctc ctgtatgtcc actgataccc gttggcgcgg gtatcaggtt ttaatcagcc
    12781 cttaacgcgt ccgaacttac tcataatttt tgagatagca gccggatcag ccgttgaaca
    12841 ctgattcaga aatgctttac gggcatcaaa tgttttactc cagcgaatgc ttcctttttt
    12901 ctttttcaga atattaaaaa aatccttttc agcacttctg cactcactcc ctccaccttg
    12961 cccgactgct tttccatata agcaaagtac cgaagcgcaa ggatcagatg cattagcatt
    13021 tgttgatatt aatcctaacg ccagcaaggc aggtaaaacc actctatttt ttccagtcat
    13081 ttttcacctc gttttgtgct ttaatgaaaa ctatttttcc ctgttcaggt aaaaaacttt
    13141 tctcgtttcg ccactccacg actgctgttg ccccttccat cttcaacaga tctctgattc
    13201 gaaaccacag cccagcatca tgtgttgtca ctacaatcct gtccgcatga gccagagcgt
    13261 aataaaacgt cggcgttctg gcctgtactc cggataagga agtgatctca taaacccatc
    13321 ctttaccgct aacgtctgac tttatgacac cgtgaaaatc ggggacatat ggcaatgagg
    13381 gattgatcac ttcatttgcc ttttcaaact gcacgggtgt tggctctggc ggtgatgaac
    13441 aaccagcaag gatagaaatc aataaaaaca ctaacttctt catcatgttc tcctttatgc
    13501 cgctttatcg agatcaggaa cttcttttac tgccagatac ccttttgcga cgatttcatt
    13561 ttcaaatact ttctgtggag gcataaccaa ctttcctttc ttcctgggga aaatgtttgc
    13621 aagttccgga gcaaccttca caaaatcacc aaagaaatac tcatggagat agtagaatgc
    13681 cttttcacaa cgaatcgggt tttctccctt cattgccagg atttcttcac taaatttcag
    13741 tgtaagtagt tcatttgttg ttaccagtgg acgctcaata aggctttctg attcagatct
    13801 cgatgttcct ccacgactac gacttttgct tttcgaaccg atagttactg ttcgtgttcc
    13861 catcgcttct gataactccc gcgcatcttt aacatcagaa gcggaataaa cgattttaca
    13921 gggtgcactt gccagtaatg ttttactgcc tgcataactg tagatttcct caagctgaga
    13981 gccgacctgg taaacaatca acaatttcag cttgtaacca gcaatgagtc cagatttact
    14041 cttgatgtaa tctacagcac caattgacgg gaattcatcc agtagaaaaa ggcagtcatg
    14101 ttttatttcc ggatcatccg ttggcatctt ttcaagattc acctgtatag caagctgaaa
    14161 gaaaatattt gttatttttt cagccaacct ggcattatca ccagaaatac agaaataaat
    14221 tgtcatccgc tttttccgca cctgccgcaa atcaaaatca tttccatcag tagcatttgc
    14281 cacattgggg ttagcaaacg gtgataatcc attaagaaag ttgcttttga gttgtgcttt
    14341 tgttttatcc tccatctcaa tgaatgcctt aaggccagcg acaccaatat cagcaagcgt
    14401 tttgtctttc ccccttaatt ctgaacgaat atcattcatg aatgaaagaa gatgttcggg
    14461 ttctgcacgc tcaaaaagct gattaatttt agacattgag aagacaggct caatatcaag
    14521 cgtttttagt ccagccttat cattcttaat cataaaccat aaagcgacag cataagattt
    14581 aaaaacaccc cccgccgtct tattaagata gctaccagca tccgcaccat ctgtcttata
    14641 ggggtaaata atcaacgcaa gattttcaag ctgattaaaa ccttcactcg ttcccatgct
    14701 gacataatac aaagggttaa agcggtgcgt taccggacta aagggatcaa atttataaac
    14761 ctcatgacct aaaatcacct tcctgataag actggtgata ttaaaacact ccctttttgg
    14821 atcgagaata ataacactcc accagtactt cataagatta gtgatgacta tacctgcacc
    14881 tttaccagcc cttgttccgg ctcccatcgc actgaatgct gctcccacat agcgtatgat
    14941 tttcttgtct tttataccaa cgataatccc gtctttttcg tcaccgtcaa gcgtgaaact
    15001 gttagactca cgaatatcat tagttgtcgc aaatcgcgca tcaccaaaaa tattatcttt
    15061 cttctcgttc agttttatca acgccgcaac agggataata agagacacga caaatcctgc
    15121 tccaagtgca acaaaggcta aaactctgat atccgtagtc acaacagata aagtaaggct
    15181 gtctgtaaaa atatccatcc tgtacgcatc aatggatctg aacacagcct tcaatggttt
    15241 cagattcttt atctggctga agtaaagcac aaagaaaata aatgaacctg caaaccatgc
    15301 cgcaacaagc ataacagcga atataaaaac agattgcgac ttattaaaag ttttcacatt
    15361 cagccccttt gtaatagatg tcgctcatat gcctgacaac accatccctg cctacatgaa
    15421 caacaatatc aatactgtcc ataatgattt tacgaagaac atcaaaagga agattttgac
    15481 attctctgtt ctgataacat tttgtcacca tctgaatgat ggcatctttt gccgaggccg
    15541 cgtgaatgga cgtcatgctt ccactgtgac cagaaccagc aactttcaca aaatcccagg
    15601 catcgccacc tttaatttcc gccagtaaaa ttctgtcagg tttcattctc aaacaggatt
    15661 tgattaattt cgctgctgtt atgatagagc cggaatcact gccggattcc gaagggtaaa
    15721 aaagatgcac ataattttta tgaataaaaa atttgatttc ctccgcatct tcaatcgtaa
    15781 tcagccgcgt attcaatggt atgaaatcga taagactctt catataggtt gttttaccgg
    15841 aacccgttgc tccggcaaat acaatggttt tccctgctgc tacggcaagc tccataaaag
    15901 cagcgatatt tctcgctctg aataaatcaa gaagctcatc atcgtatgtc ctgtgttttt
    15961 ttcctgactg gacataatca taaaaaccgt tcgccacata ttctttatgc gttatctgcc
    16021 gtgtcgatgg tttacgtata gtgattgaaa tagtatttct ctcacatgca ggaggaaaaa
    16081 cgacctgtac acgttctcct gattccagtg ttgctgaaag taatggcttg gtatcgccga
    16141 tttcatctcc ccgaaatgat gccagcgtct gagaaaaacg tttacaaaaa tcaaaggtca
    16201 caccgtcatt attatggaat gtccagtcac cattaatttc cgtccagatt tcaccaggac
    16261 ggttgacagc aatttctgtt agcccttcaa tatcaagaaa agactggaat acttgatttt
    16321 tataaaaatc gagcgattta ttgctaatgc tgacaggaat aacgccattc atactatttt
    16381 acctttaact catagatatc agaaaaatca atgtcttccc cgaccataat agtgattatg
    16441 tcaccttggt ttttatacat tgttggagga atatttatcg aattctcaag tgcgacttta
    16501 gccatttcag ccattgcctg tcgtgagttt tcggtataat ccacattacg atctttacca
    16561 ggtgcattat ccgccgccgc cgccgctaca tcctgaacca tactaagcat aagagaacca
    16621 ccaaaacgct cccagaagtg agaatcgatc catccatcaa tgcctgcttc ccccagggga
    16681 ccagccgctg ctgtatttac catatcgatt cgtttattat caggcgtccg caactgctcc
    16741 cacataacga acattctgcc ctgtccatga ttcagcgttc cggtctggta acgaccaaaa
    16801 gccttagttc cctgttccag taattttgtg tggtgattag cactccatac atcttctgta
    16861 aaaacacaac tgatacgacc tgcaacatca gaaacaaagc gtgtggttaa agcacacggt
    16921 ataagcctgt tttcctcaat atagagatca ggatcgagat taatcgcagt cactttcatt
    16981 cctgatttac caggcacatt ttcttttgat tctttccttt cttcctgtgg ctctgatgtc
    17041 tgcgatgtcc tgctgctgcc actcgcgcca gtctgactcc ctccctgacc agcaacaggt
    17101 attgacaaat atttcctgaa cccctcctgt ggcacttctt tatgaccgtc agaaacagta
    17161 ttaccagaac cagtagcgcc tggattgata attgctttgt tctcaacagg gtcataattc
    17221 tgaccaagac cagtacggat tttacttacc tgagaaatgc tggtatcgcc tgttgattct
    17281 gatgctttct gctggtcatc tgcaaaataa tttttatatg ttttcagcgc aaaaatacca
    17341 ataactaata cagccatcag accaaacaga aagccagaac gcatacgatt atttttgttc
    17401 ctgatatcac cgatatcatg aacgggttta ctttccagct ttgactgatt cgcatcatca
    17461 actaaatcat ctgttttttt catatcagtc attattcacc tcaacacgtt ctaccgcagg
    17521 agaagatgta ttcctctggt cggttaatac ttttccgaaa gatttattca caacaccaac
    17581 aacgctgttt ccatagcgta aaacaaattt gtcattaacg ttgtggataa ccatgatttt
    17641 atacttgcct tttgtttcca cattaaaagc aagtgtctgt tcctgaccat ctcttacagc
    17701 aaatggagct gggaatactt tccctgagtt aaaacctaaa aaggtaaata tgccattatc
    17761 ataagcaaaa tcaggggcga tacgacgact gtcatatttt tcattgactc gcatgaaata
    17821 atcccagttt ttaggcgcat cggctttttc aaaactttca gaaatcagtt tcttctgtct
    17881 gagtttttcc tgtttttctt ttgccagcct tacttcttct gcattctttt tcgctatttc
    17941 tgatggatag cggaattgta caacataagc aggttgcgct tgtttttctt ctgacagaag
    18001 gattagatca agactgtaaa tccgctttgt tgttctgaca aaaagattcg tgtcccattc
    18061 cttttctgtt ggctggaaaa cctttttcag tttttcgcct tcatcgcctt cctgttcctg
    18121 aacaacggga cgcggattga tgtaaacaac attatcatcg gttgtgattt cccatccggc
    18181 aggaaaacca gcctttgcac tgataacagc ctcaccttca tcaaaaacaa ctgatgtgag
    18241 aaatcctgtc tttgaccgga taacggtcgt attcgcaccg ttataactca cagtctgcat
    18301 ccttgcatca aatccagaag gtaacggagt cgctgctgaa tacacaagcc caggaagcaa
    18361 taaacatgta gcaataaata atttcttcat cattcccccc tgacttcccg atctgtctgg
    18421 taactggtta ccgtaaaacc gagaggatta agctcacgac tggaactttt aaccgacttt
    18481 tctggttcaa aacgatatgt cacacgagcc gtccagtatg aaactgaaac ctgtccggtc
    18541 gtgaagtttc tcgtcgtttt tttaaaacgt acagaggcca gtttatctgg cgcggttgcg
    18601 tcagaaataa tattggagat gacctcaacc gcaacgtaag aaccgttatt atgataaatt
    18661 ttatcaggag ccatatcgct gttatacaga cgaagataat cgtcctttac tgtattcgag
    18721 ctatatagct gtacagtctc ataatcatac tgaagagacg gataattata accttcacgt
    18781 aaagtaatat acgtgtttac aaaataccgc ccaagcgctt cagattgcag gatcttttcc
    18841 tgtttaacct gagtaatcag ttctgtacgg cctgtttttt cgccaacagc cacaatagca
    18901 agctcttttt cttttaatgg cacaagaaca caaattgcag ccccaagaca taaggacagt
    18961 aatgatgctg ccactgcaac acgccatgca tttttcttag accgttcatt ttctgcatag
    19021 agttgtcttt cgaaggattt agctgtctca atcttctttt cgattttttc actcataact
    19081 gattacctgg ctgtggttaa tgggttccag ttcgccggaa acatcagaag gaagggtatt
    19141 ccttgtggta catccggaaa tctgaaacag gaaaaacaaa agaatcacac atcgcataaa
    19201 ttcaccttat taagcgttca cgaacacctt taagagataa aggccaagat gaagaaaaag
    19261 aatcccacca ataagaagca aaaagcgaag atccataccc tttctccatt tccccgcaag
    19321 ccctaaacca agtcttattc ctgcgaagca ggtaaacact cctaaaagaa ggattaggac
    19381 aaaataaagt atcatgacaa aataatgaag catgtcacaa ccagctctga ctgattccac
    19441 ctaagacctt aaataaggta attcccgtga ttaaaataat aagctgggtt attagctttc
    19501 tcttcctgct ccatccggag gtgatatgtt tatcaacaaa cgtcagtatc cagttcaggg
    19561 acatcacata aaaaaaacgt ttcattatat tcatgcggca ttcctcgctc gtgctttttc
    19621 cagggatgca agtcttctcg ccttcatccc caaagcacct gtcggagcca gcgcctgata
    19681 tcccttttca cccgcccatt cagcggctgt tcgcgcacca cgccccatca gattccctgc
    19741 tttcccagat aaagaattca tgcccgttct tccggccatc cctaagccaa aacctaaacc
    19801 agcacgaccg ccaagcatca gagacttacc agtagccata ccagcagcac ccagccccat
    19861 catggctgcg ccctgaacag caccttcaac acctgcacct gctaactgga cagcaaaacc
    19921 tttagctata agaaccaata aagcccccaa aaacccagcc ataaagccca tagcacctgt
    19981 agatacaaga tttacatttc ctgtttgtgc agcacgaata gcatgggaaa ggatcatccc
    20041 ttgaaaatcc attgctatcc gaatcactaa actggcaaat agaactgtca gaatactgga
    20101 gaataacgat tgtaaccaat tattaaacat gctccgaata aaaccaaaca taagacaaaa
    20161 gataaatatc ggtgctgtaa taatcaggag tttcatcgtt acatctgctg ttaaaaaaac
    20221 aaccacacta acagccatta aaataagcga gccagtccat attaaaaact gtccaactac
    20281 accttgatct ttaacatatg ttgatttatc taaagagtat atttccgcgc caagattttg
    20341 cgtacttttc cataagttat ctaaagtttg ccagacactc acccctccag aaaaaccatc
    20401 tttcatgcct tgcaacgcat cagtggccgc agttaaatac ccatccgcat tagttataaa
    20461 catgattatg attgcaaatt ttgatagatc ccatactaaa tcctgcaagg gcgtctgagt
    20521 tttactggca agtatttgat atcctttcca tagtacatat aacgtaaccg ataccgtcgc
    20581 taacgacata atatcattag caaattgtgt ttgataactg gagctgattt tatctaccga
    20641 atccataacg gtagaattat atttaacgaa aaacccctga gccatatatc caccttttaa
    20701 tcaaaagtta atggttcagc ttctttctgc tgtttaataa aggcctcttg ttcttgtgtg
    20761 atcaacatct catgatctgc tttattttta ttattccaga catcatactg catttttatc
    20821 gcctgcattt ttaacgactg cgcctgtaat gcgttagcca aatcctggga ttctttgata
    20881 tcctttgaag cctcaattcg gctacttaaa ctctgaatct gagacattgc atttgataac
    20941 tgcttattga tctcatttcc ctgttcaata ctggcagccg ttgacacaat ccgtgcttta
    21001 cagagattat cattacgttc atagcccgta ttacacatat cgaaggctcc atacttgctg
    21061 aacagatttt tagcctggct gctcaatgca ccttcgggat tactggtaaa atcagaaata
    21121 taactgttcc cctgcttata aatatttgtc agttctgatt gtagattact tagttcattt
    21181 gtgaatgccg caatattcct tatacctgtt gccgtggcta actggtcttt atatgcctga
    21241 atctgactct gataatgttg cgcagtttcg gaccattgtt tgagtcgttc tgtccatctt
    21301 gcggcttcaa cagcccattc cggactggca tcaatagcaa ccggaattcc tgcgctacta
    21361 taaaatgaag ccgtcaataa tgatatcagc ataaatgttt ttacacgctt cataaattca
    21421 cctgtcagat tgcccgttcg agatattcag gaacccagtc atcaggctcc attccttcat
    21481 gataaatact ttcaaaaatt tccagattat ctgcactagc agacaatatt ttcagatatc
    21541 caccaagtcc cgataaatca agtgtcgcaa gtgctgaaaa tgatttacca tccccctttt
    21601 taagactgct ttttttaatc aggaactgtc tcgacattgg atcaagattt ttaacaatat
    21661 taaattcttc tggtgtcagc ttaagccctt ccacataatc attgtaatcg gcatcagggt
    21721 tagccagata aatactggtg ctgcatactt caaccacagc ccgtgaaatc ttattcttca
    21781 gtatttcatc cggagactga gttgccggaa taaccagccc gttcagctta cgaattgttt
    21841 tcagcttgtt atagacaaag tcgctgaatg cttcatcctg tagccatttc cagaactcat
    21901 ccaggaagat aacaagacgc cgaccgtcaa gaagctgcgt aatgcgatac agcagataga
    21961 aagtaattgg tgcacatacc atcggatcat caagaaactc ggtgccatca atgccaaaat
    22021 tattgacatg ctgaatattg aatgtgtctt tagcattatc aaaaacccag ccatgagcct
    22081 gaccattagc ccattgggac aaccgaagga taatcccgtt ctcctgctca tcccgatcat
    22141 ctctctgcat aagatgctca agcattctgg taatgccata ttcccgcatt tcgccaggag
    22201 gaaaatccat cacagcatca acactttcgc taataagcaa tctttctctt gtcgataatc
    22261 tttcaccatt tctggtacac agaatctcca tcagttgctt gacaaaaata cggttacgtt
    22321 ttgttgcctc cagagcaaaa ggattccatc ctgtcggctc accgcttttt acggtgtaat
    22381 attccccacc aagcattcgg atacataatt cagcccctct gtccttatca agaaagacac
    22441 acgttaactt cttatttttt gcagaatcag caaaagtttc ggggttattg tatttctgta
    22501 atgaacaggc aagatatgac agaaacattg tcttacctga acccgccgtt cctataactt
    22561 tagtattagc aagattcttt tcacctgttt catctttaaa cagtactgag ttatgtaaat
    22621 tcagataata cgcctgtttg cttggtgttt tgagaatagc aaccgcttct gtccagcagt
    22681 ttttatcgcg tttcccctgg tagaaattat ggaatgatgc cagttcgaca aaatttacat
    22741 tcgataccgg agataaacgt ggccttaaat gaaaaaccgc cggaagctgg gcaaaatatg
    22801 ccgctggcaa tgacatggtt gacaatgccg gaataatgcc caggtcagta aagtctgccg
    22861 taattttact tacagattcg tcaagctccc tgatactgtc cgccattacc attagcgtga
    22921 aatggtagta accaaaggat atatcaccgg acacaatatc attttttgcc ttttccagtt
    22981 cctgcaattg agtaaccgca tcatcttctg tactctttaa ctgtttttcc gcacgtttaa
    23041 tggtactcaa tgcttctgat ttgctcatgg atgtatatga atgcgtaata atatagtcag
    23101 catcagaata ctggagcaca tcaaaaacac cggatgcagt ctcactacag aaatctttaa
    23161 tttcaataga acgaaagaat ttatttccat caatgcggca tatctgtccg gtatcatggt
    23221 taaaaaacac atccactcca ccaagaacgt tatacaccgg agaatcagtt actctgattt
    23281 tttgcaactt ccctgtcaga agataattat agaatgacag ttgagaagaa aatacctttc
    23341 cgttctcctc atacatacca agaagtttac aggtaaattt atcaagagcg ccagagaatg
    23401 tattaatcat ttcattcatt ctcttcacat gaatgttaat atcatctttc ttttctttaa
    23461 tacttttttt gcgtttttcc agtttttcta cacgcccgtc tggtctgtag atcagcgtaa
    23521 agtaaagagt gttccccttg aatttatttt tcttcatcga accataataa caatctgata
    23581 ttatatcagc atatttatta cctgattttg tggtaaaggc atcataaaaa ctcgccctgc
    23641 atgaatggtt ataaaatgaa acgggcagtc cggaaaatga acgaacaaga gtggcaacct
    23701 gtgagtcaat agtttctaac atctccgcag agattgactc aaagttaatc cccatcagtt
    23761 gccatgtcgc tacataatca ccgttttttg ttttgacgat gtttttatca acgtggcttg
    23821 aatatgggat atatttacca gtagtgattc gctcattcag tttcatggca ttaacaaact
    23881 catcaatttc aatagcttca tattgtccgg agaggaacgc tcgtgcgcca aaaaaacgat
    23941 tacacactga gtttccgagc attttcagct taagaaagta aaggttaaaa atgtggtcgt
    24001 cctgtttagt tatcattctg agaacaaaaa ccgcgacagg aatagccagc cagatcagct
    24061 tgctgatata aacccccgcc agaaataacg cccctgcaac cacagtaaga gggacgacag
    24121 gaacacccac atacatagca gggcgtgtca tcgctttata aagtgtagac atactatcgt
    24181 ccccgcttta ttagttaacc atcattgatg caacttcagc cgcactggcg ataacaattg
    24241 ccccgatgat gataggtgag cattcacgaa tcgtgcttcc accaaacaga accttgtagc
    24301 cgacccacag acaggcaata gtcaccgtga ccagtgaaag accactaagc cctgttttaa
    24361 ccttttccag cagcgtttcc gcttttgaaa atccatccgc aaaggccggt gatgcaactg
    24421 ttgccccggc gactgccata actaccttat ttttacgctg cattacagca gtgacagatg
    24481 aactgatacg attacatact gatttcttaa atgacatttt ataagtcctg ttaattaata
    24541 acttttgttg gatgtgagtt atcaacaaaa gcagatttca gaatggtttc gggccagata
    24601 actgacgcat tgtgtgaacg attttttcgc tgttctcccc cgttgcttct tgtacctggt
    24661 acaacatatt ttttttcgtt ccctgtataa ccaattcgct caacataact tgtattatta
    24721 aattcttttt cttttcgctt accgttacta aaatttcctg tatagtaaca actaagcgca
    24781 ttctccaggc tcctccctcg tttataacaa tccacgataa tcttttcgaa aacagacaag
    24841 ttttcacacg gatcaaggag tttttctgcg gacgtggaat aaaaagaaaa attactgttg
    24901 gtaatctgca tcaatccgac agaataacgt ctttttctgt tttcaatttc tctgataact
    24961 gtaagagctt cctccttact tttgggcata tgactgataa cacttttgcc atagctaccg
    25021 cgttcccttt caggaataat ttcagccaca gcataaggat tgaagccact ctcgacccgt
    25081 gcaatatcat ttatcgtatc cggatgaact gacgccgcac acagcaacaa tgctgggaat
    25141 aaagacgcca tagctaacgc tccatatatc catcaatacg catactctgc attaataagt
    25201 aaataatttg cttcctctta ctaagcaaag atgttttcgt tttctcttcg ataaatgcat
    25261 tcaaccttgg atcgagtttt attgtgacat aaaccccttt ttccgatgac ttatctggtt
    25321 tctccttcat ttaatcaatc cactataagt gtgttttgga tttgttttag atgcgaacat
    25381 gattcgcaaa ataacaatgc tcaacaatac gatcaaatac cattgacaaa atggtcatgt
    25441 tatgattcgt gtgatcatgc aaaaaaaagc gcccatatgg gcgctttagt atgatttaaa
    25501 cattagttaa tcgaatgtag ggactggagc atccttctga gactgaataa acaatgcttg
    25561 tttaacattc ttacagttct gagaatcaga gccagacttc tcacactctg cctgcttttg
    25621 tttagcttcc gtaagatgct gcccccacca atccacactt tttacttctt cactacaacc
    25681 aacaacgaag aacggcaaag caaaaagcag atattttttc ataaattccc cctctcactg
    25741 atcctggaaa gacttgacac gagaagggca ttagtccgaa tgagtaccag tgttctgatc
    25801 cctgccaacg agacacctcc cagttcagct cttacagaat caagatattt tctgacagtt
    25861 gaaggcttta gtcctttgaa atcagcaatt tcattcaggg tatagccata agcgtacatc
    25921 agaataatgt ctttttgttg ctcactcaaa tgttcttcat cactaaaaat ttgatgaagt
    25981 gcagaaatgc agatagcatc atgctcagtc atgataacct ccctcgtagg ttgttgtgat
    26041 tagcaggtga gcaaagtagc cgctttgttc acctgcgccg aaatcaaatt aaaatcccag
    26101 gcctttccag ttcctctctt gctgtattct tagtttttta tgttcttctg aatcctttgt
    26161 aaacccgtgt tcaagagcaa acccgatatt gtgaccttgc tcatgttttc tggatagctg
    26221 ctttttaacg tcgctctctt ccggctgcct ggtttcacgc tcatgaacct gacgttctct
    26281 gttccgctca agggcaatat tttcaatctg ccagttattg cgatgcgttt ccctgtagcc
    26341 tgcaacagtt ccatcatcat taaacatcgg gctacgtaca agcgtaggag ccagtttttt
    26401 aactttgata agcgttcctg gctggagctt ttcagactcg aaatgatttt tcaggtcttt
    26461 accccaaatc gtaacctgct ttccaccttt taaagtttca taagtgagga aaggtgttcg
    26521 tttttcccct gccttattct gatatgaagt ctccccaaaa tccacaacct tgtaaacatt
    26581 ccggagtctt ttcggatttt cagcctgtaa tcgctcaatc tcccgtttac gttcgggatc
    26641 ttttttccat gtagcggtaa catcatatcc catacctttc agttttctat gaaaaccgtt
    26701 tctgaacttc ctggtttcag acttagtaag tcttaatctc cttcctgttt cttcatcacg
    26761 aagtttaata ttaacatgta catgaggatg atcttcttta tcatcatggt atacggcaac
    26821 gaaagcatgg tttggataag tctctttaag aaattctatc gtagctttca gagcgtcttc
    26881 cctgcttact ccagcttctg gtggtggtga aaagaccatg tttttcacat gatcgatgtt
    26941 ttctccacga tatgttctgg agtaatcatt tcctttagat aaagttgaag taaactccct
    27001 gttaaagtct tctcctttcc ctgtttgctc ggaacctttt ccatcataac agtaagcctc
    27061 aagttcacca ttacgggtaa tgtaatcaat accgtttttg atacctgcgg cagttttccc
    27121 tgaacctgtg attttaaaag tgacttcttt tgttggcgtc caggtatatg ctttcttgtc
    27181 tgtgccatgt ctgactttat gattaaaagc cgatttactg gcatgtatat gtcttcccga
    27241 taacttagga gatttcggat cacgcccagt ccctttagcg cgtttaatac ggtattcctg
    27301 ttctacatac acacccataa atcacccaaa tctgttaaca acactcttgg ttaacgtttc
    27361 aagttttgaa tcaatttgct tacatagttc aatcacttca ataacgtctt ttctgaaatc
    27421 aggatcgtta accgtgcaaa atctttcacc ttttatgata aaatgaatgt tgcgaccaac
    27481 acgatctaca gcagttgata attttttaag ttccttaact tcatcagggt aaagacagac
    27541 ttcattcttt aacaaagact gacgcacacg aatagcaatt tctctgctca tgctcatgcc
    27601 gtgaagggca gcattacgtt taacaagttc tatttcatga gggtagagat aagtaaaaac
    27661 tggttttgca gcctcattct taacaggaat agatactggc tttgttggta catcaagaac
    27721 tttcattttt gcgaatacat ttacactatc aactccatat gaagctgcat ttactctcgc
    27781 aagttcatat tcatcatcat ttaatctaaa acatacattt ttcattcttt caccatagag
    27841 cctgcggcag gcacaggcaa aaacatcgtt tttggacaat ctactgaaaa ttgcatgcaa
    27901 tttctagtgg attgtctatc ctgcattgat tgtaccatat gtggcactgt gcctggctat
    27961 aaaatcgtcg ctgcatatgt ccagatttcg ttgctgaaag gttctttcgt cttcgaccat
    28021 ctggcaaacg tatagctgaa agatttgtga aagaaaaaac aggcctggca gggctgggaa
    28081 ttgttacggc tgaagccgta acgcttttta agtcctcgct gcgctgcggg gcttccggcg
    28141 caaagcgcct cccatgccct tacagggcat aaaaaacaag ggcaattaag cccctgcgcg
    28201 atgattgctg acttcttttt ccagagcctt attaacaaag gcattcaacg ataaatcttc
    28261 ttccatcgcc atttctgcta cccgacgatg cagttctgga tcaagcctga cgttaaacac
    28321 acctttaaac ggggtatcag gttcctttcc atcctccaca caagactgta aatacagatc
    28381 aacagatgtt ttaaactcct gttccaattc agctaatgta gaagcctcat aagttaccag
    28441 gtcccgaata aacgccagtt ttccatacag gacattattt tcaaaatcgg gttcaaccgt
    28501 gcctaaatat cctttatatt ttaaatgttt cataataatc cagcctcttt cagattctgt
    28561 ttaattgctt tcagcgtacc acctttgata taactttccg gatgtggacg atgcattaat
    28621 atggtgtgat tgatttcagc attgaaaaac cgcactcttg acccctgcat ttcctttttg
    28681 acatatccca aagaggaaaa caaaacgacc agttcatccc attcaaacgt ttttttactg
    28741 tttaaaaact ttgcctgtag cttatctgtt ttccccataa atccatcccc tatagatatg
    28801 caactaatta tagttacaca tggcattttt gtcaaaaaat acaaaggggc agaaagcccc
    28861 tttttttatt taaaacattt catacccttg caagtagttt aaccaccacc ggaattgatc
    28921 ccattcacta aatttcattg ccggaattct ggtttcccag cctttgacaa gggaatcagc
    28981 aagctgtact atctgctgcg ttgacattta aaaacctcat tcagttttga ttgtcgataa
    29041 cgtgatcagg ttggtggaac aacctgatca caccttgaat agtcagtcat taccctcttt
    29101 cttttctata gttactgtgt atccgtaaag agtcaaaatg cgataagcat ccaacgcctc
    29161 acgctcactt ttaaatgttc tgacttcgtg taatccatta cctttatcaa gggatacaca
    29221 aaactcataa tcttcttcta cttcgatctt tttaattaat ttatcaagtt cggctaatgt
    29281 tcgttttggg tcaaaaagaa atgtgcattg tacaatggcg tttaaatctt cccgatcaag
    29341 ctctaacaga gcatcattat aagcagcttt aaaacagcga ttattacgca gatcagatgc
    29401 aaactgcatt gcggcttgtt ttaacgtttt catgaattac ccttgcattg atgttaatag
    29461 acaatttcag aatcaatatt gaaatttcca gaaaatgcag ggttccctcc cctgcattta
    29521 taatgcttaa cgaaggcact caataaaatc tttaaaactc aagcattcat cgccatcttt
    29581 taacgactca aaatattttt catattcgtt cctgtagaaa aggactaaat caacatcact
    29641 ctgcttaagt tcgccaaact gaggaacaat tccggtttta tagtcacgaa ttttattgtt
    29701 atcagtaaat atgacaacat actgttgtcc cgtatcctta tgcataagga taatagtgca
    29761 aagcgttact cgttcgaatg tgaaaacatg attagtggtt gaagtaaaaa cagatgagca
    29821 tttcatttt
//
LOCUS       DAEVZA010000049        29818 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEVZA010000049 DAEVZA010000000
VERSION     DAEVZA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.049"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..700)
                     /gene="argC"
                     /locus_tag="LAF92_002262"
     CDS             complement(<1..700)
                     /gene="argC"
                     /locus_tag="LAF92_002262"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBI9791263.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLG"
     gene            854..2005
                     /gene="argE"
                     /locus_tag="LAF92_002263"
     CDS             854..2005
                     /gene="argE"
                     /locus_tag="LAF92_002263"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBI9791264.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            2357..5008
                     /gene="ppc"
                     /locus_tag="LAF92_002264"
     CDS             2357..5008
                     /gene="ppc"
                     /locus_tag="LAF92_002264"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBI9791265.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            5191..6924
                     /locus_tag="LAF92_002265"
     CDS             5191..6924
                     /locus_tag="LAF92_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBI9791266.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAMAVLLWTRLRPVYIPKPWRYIVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            7073..7924
                     /locus_tag="LAF92_002266"
     CDS             7073..7924
                     /locus_tag="LAF92_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9791267.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(7911..8252)
                     /locus_tag="LAF92_002267"
     CDS             complement(7911..8252)
                     /locus_tag="LAF92_002267"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBI9791268.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(8254..9132)
                     /locus_tag="LAF92_002268"
     CDS             complement(8254..9132)
                     /locus_tag="LAF92_002268"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBI9791269.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(9098..11395)
                     /locus_tag="LAF92_002269"
     CDS             complement(9098..11395)
                     /locus_tag="LAF92_002269"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBI9791270.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(11446..11766)
                     /locus_tag="LAF92_002270"
     CDS             complement(11446..11766)
                     /locus_tag="LAF92_002270"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBI9791271.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(11781..12860)
                     /locus_tag="LAF92_002271"
     CDS             complement(11781..12860)
                     /locus_tag="LAF92_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBI9791272.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            13169..15670
                     /gene="ptsP"
                     /locus_tag="LAF92_002272"
     CDS             13169..15670
                     /gene="ptsP"
                     /locus_tag="LAF92_002272"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI9791273.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQQQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            15682..16344
                     /gene="fsa"
                     /locus_tag="LAF92_002273"
     CDS             15682..16344
                     /gene="fsa"
                     /locus_tag="LAF92_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBI9791274.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            16355..17458
                     /gene="gldA"
                     /locus_tag="LAF92_002274"
     CDS             16355..17458
                     /gene="gldA"
                     /locus_tag="LAF92_002274"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBI9791275.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            17733..18350
                     /locus_tag="LAF92_002275"
     CDS             17733..18350
                     /locus_tag="LAF92_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBI9791276.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(18377..19282)
                     /gene="yijE"
                     /locus_tag="LAF92_002276"
     CDS             complement(18377..19282)
                     /gene="yijE"
                     /locus_tag="LAF92_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBI9791277.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(19376..21556)
                     /gene="katG"
                     /locus_tag="LAF92_002277"
     CDS             complement(19376..21556)
                     /gene="katG"
                     /locus_tag="LAF92_002277"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBI9791278.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(21885..22775)
                     /gene="metF"
                     /locus_tag="LAF92_002278"
     CDS             complement(21885..22775)
                     /gene="metF"
                     /locus_tag="LAF92_002278"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBI9791279.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(23124..25556)
                     /gene="metL"
                     /locus_tag="LAF92_002279"
     CDS             complement(23124..25556)
                     /gene="metL"
                     /locus_tag="LAF92_002279"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBI9791280.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(25559..26719)
                     /gene="metB"
                     /locus_tag="LAF92_002280"
     CDS             complement(25559..26719)
                     /gene="metB"
                     /locus_tag="LAF92_002280"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBI9791281.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            26996..27313
                     /gene="metJ"
                     /locus_tag="LAF92_002281"
     CDS             26996..27313
                     /gene="metJ"
                     /locus_tag="LAF92_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBI9791282.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            27373..27981
                     /locus_tag="LAF92_002282"
     CDS             27373..27981
                     /locus_tag="LAF92_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBI9791283.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            28126..28801
                     /locus_tag="LAF92_002283"
                     /pseudo
     CDS             28126..28801
                     /locus_tag="LAF92_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(28888..29211)
                     /locus_tag="LAF92_002284"
     CDS             complement(28888..29211)
                     /locus_tag="LAF92_002284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791284.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFYESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRK
                     KSRMR"
     gene            complement(29213..>29818)
                     /locus_tag="LAF92_002285"
     CDS             complement(29213..>29818)
                     /locus_tag="LAF92_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HBI9791285.1"
                     /translation="QQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLS
                     PVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPIS
                     SNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNS
                     YMNGMEKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT         7275 a         8184 c         7502 g         6857 t
ORIGIN      
        1 tgcccagatg tggggtgaag ataacgtcag caccgaggtg tgtggcgatc tctggttgat
       61 ggcgatgagt aaagacgcca tacggttgca ggctaacttc acaaaagctg tttgaaatgg
      121 ccgctttacg ccctgcaccg ctcacgccgc tggtggcgtt gatcaccggc cactgattga
      181 ggtcaagaag atcggcatca atcaacggtt tcagcgccag ctgtgccgcc gtcggataac
      241 agcccggcac cgcaatcaaa ttcgcttctt ttaatttatt accgcaccac tccgccagac
      301 cgtaggctgc ctgttccaac agttccgggt attgatgggt aaagccgtaa tatttttcat
      361 agaaggtggc gtcgttaaca cgaaacgcgc cggaaaggtc gaacaccacg cagcccgctt
      421 caagaaactg cggcgctaaa tcgtggctaa cttcatgggc ggtggcgaga aacactacgt
      481 ccacccctgg gctaaactcg ctgatatccg acatcggctg caacggcaga tcaacgatgc
      541 cttttagctg cggatgcaaa tcggagatta actttcccgc atcattgctt tgcgctgaaa
      601 cagtcaaagc ggttatgttc atatgcggat ggcgatttac ataggtcact agctctgcgc
      661 cagcgtagcc gctggcaccc acaatcagcg tattcaacat cggggctatt caccttctta
      721 tgtctggttg ccaggttaaa cgtaaaacat tcaccttacg gctggtgggt tttattacgc
      781 tcaacgttag tgtattttta ttcataaata ctgcatgaat attgatacta tcatgaccag
      841 aggtgtgtca acaatgaaaa acaaattacc gccatttatc gagatttacc gtgctctgat
      901 tgccacacct tcaataagcg ccacggaaga ggcactcgat caaagcaatg cagatttaat
      961 cactctgctg gcggactggt ttaaagattt aggcttcaat gtggaagtgc agcctgtgcc
     1021 aggaactcgc aacaaattta atatgctggc aagtatcgga cagggggctg gcggcttgtt
     1081 gctggcgggg cataccgata cggtaccatt tgatgacggt cgctggacgc gtgatccgtt
     1141 tacactgacg gagcatgacg gcaagcttta cggcttaggc accgccgaca tgaaaggctt
     1201 ttttgcgttt atccttgatg cgctacgcga tgtcgacgtc acgaaactga aaaaaccgct
     1261 ctacattctg gcgactgctg atgaagaaac cagtatggcc ggagcgcgtt attttgccga
     1321 aactaccgcc ctgcgcccgg attgcgccat cattggcgaa ccgacgtcac tacaaccggt
     1381 acgcgcacat aaaggtcata tctctaacgc catccgtatt cagggccagt cggggcactc
     1441 cagcgatcca gcacgcggag ttaacgctat cgaactaatg cacgacgcca tcgggcatat
     1501 tttgcaattg cgcgataacc tgaaagaacg ttatcactac gaagcgttta ccgtgccata
     1561 ccctacgctc aacctcgggc atattcacgg tggcgacgct tctaaccgta tttgcgcttg
     1621 ctgtgagttg catatggata ttcgtccgct gcctggcatg acactcaatg aacttaatgg
     1681 tttgctcaac gatgcattgg ctccggtgag cgaacgctgg ccgggtcgtc tgacggtcga
     1741 cgagctgcat ccgccgatcc ctggctatga atgcccaccg aatcatcaac tggttgaagt
     1801 agttgagaaa ttgctcggag caaagaccga agtggtgaac tactgtaccg aagcgccgtt
     1861 tattcaaacg ttatgcccga cgctggtgtt ggggcctggc tcaattaatc aggctcatca
     1921 acctgatgaa tatctggaaa cacggtttat caagcccacc cgcgaactga taacccaggt
     1981 aattcaccat ttttgctggc attaaaacgt aggccggata aggcgctcgc gccgcatccg
     2041 gcactgttgt caaactccag tgccgcaata atgtcggatg cgatacttgc gcatcttatc
     2101 cgacctacac ctttggtgtt acttggggcg attttttaac atttccataa gttacgctta
     2161 tttaaagcgt cgtgaattta atgacgtaaa ttcctgctat ttattcgttt gctgaagcga
     2221 tttcgcagca tttgacgtca ccgcttttac gtggctttat aaaagacgac gaaaagcaaa
     2281 gcccgagcat attcgcgcca atgcgacgtg aaggatacag ggctatcaaa cgataagatg
     2341 gggtgtctgg ggtaatatga acgaacaata ttccgcattg cgtagtaatg tcagtatgct
     2401 cggcaaagtg ctgggagaaa ccatcaagga tgcgttggga gaacacattc ttgaacgcgt
     2461 agaaactatc cgtaagttgt cgaaatcttc acgcgctggc aatgatgcta accgccagga
     2521 gttgctcacc accttacaaa atttgtcgaa cgacgagctg ctgcccgttg cgcgtgcgtt
     2581 tagtcagttc ctgaacctgg ccaacaccgc cgagcaatac cacagcattt cgccgaaagg
     2641 cgaagctgcc agcaacccgg aagtgatcgc ccgcaccctg cgtaaactga aaaaccagcc
     2701 ggaactgagc gaagacacca tcaaaaaagc agtggaatcg ctgtcgctgg aactggtcct
     2761 cacggctcac cctaccgaaa ttacccgtcg tacactgatc cacaaaatgg tggaagtgaa
     2821 cgcctgctta aaacagctcg ataacaaaga tatcgctgac tacgaacaca accagctgat
     2881 gcgtcgcctg cgccagttaa tcgcccagtc atggcatacc gatgaaatcc gtaagctgcg
     2941 tccaagcccg gtagatgaag ccaaatgggg ctttgccgta gtggaaaaca gcctgtggca
     3001 aggcgtacca aattacctgc gcgaactgaa cgaacaactg gaagagaacc tcggctacaa
     3061 actgcccgtc gaatttgttc cggtccgttt tacttcgtgg atgggcggcg accgcgacgg
     3121 caacccgaac gtcactgccg atatcacccg ccacgtcctg ctactcagcc gctggaaagc
     3181 caccgatttg ttcctgaaag atattcaggt gctggtttct gaactgtcga tggttgaagc
     3241 gacccctgaa ctgctggcgc tggttggcga agaaggtgcc gcagaaccgt atcgctatct
     3301 gatgaaaaac ctgcgttctc gcctgatggc gacacaggca tggctggaag cgcgcctgaa
     3361 aggcgaagaa ctgccaaaac cagaagggct gctgacacaa aacgaagaac tgtgggaacc
     3421 gctctacgct tgctaccagt cacttcaggc gtgtggcatg ggtattatcg ccaacggcga
     3481 tctgctcgac accctgcgcc gcgtgaaatg tttcggcgta ccgctggtcc gtattgatat
     3541 ccgtcaggag agcacgcgtc ataccgaagc gctgggcgag ctgacccgct acctcggtat
     3601 cggcgactac gaaagctggt cagaggccga caaacaggcg ttcctgatcc gcgaactgaa
     3661 ctccaaacgt ccgcttctgc cgcgcaactg gcaaccaagc gccgaaacgc gcgaagtgct
     3721 cgatacctgc caggtgattg ccgaagcacc gcaaggctcc attgccgcct acgtgatctc
     3781 gatggcgaaa acgccgtccg acgtactggc tgtccacctg ctgctgaaag aagcgggtat
     3841 cgggtttgcg atgccggttg ctccgctgtt tgaaaccctc gatgatctga acaacgccaa
     3901 cgatgtcatg acccagctgc tcaatattga ctggtatcgt ggcctgattc agggcaaaca
     3961 gatggtgatg attggctatt ccgactcagc aaaagatgcg ggagtgatgg cagcttcctg
     4021 ggcgcaatat caggcacagg atgcattaat caaaacctgc gaaaaagcgg gtattgagct
     4081 gacgttgttc cacggtcgcg gcggttccat tggtcgcggc ggcgcacctg ctcatgcggc
     4141 gctgctgtca caaccgccag gaagcctgaa aggtggcctg cgcgtgactg aacagggcga
     4201 gatgatccgc tttaaatatg gtctgccaga aatcaccgtc agcagcctgt cgctgtacac
     4261 cggggcgatt ctggaagcca acctgctgcc accacctgag ccgaaagaga gctggcgtcg
     4321 cattatggat gaactgtcag tcatctcctg cgatctctac cgtggctacg tacgtgaaaa
     4381 caaagatttt gtgccttact tccgctccgc tacgccggaa caagaattgg gcaaactgcc
     4441 gttgggttca cgtccggcga aacgtcgccc aaccggcggc gtcgagtcgc tgcgcgccat
     4501 accgtggatt tttgcctgga cgcaaaaccg cctgatgctc cccgcctggc tgggtgcagg
     4561 tacggcgctg caaaaagtgg tcgaagatgg caaacagagc gagctggaag ccatgtgccg
     4621 cgattggcca ttcttctcga cgcgtctcgg catgctggag atggtcttcg ccaaagcaga
     4681 cctgtggctg gcggaatact atgaccaacg cctggtagac aaagcactgt ggccgttagg
     4741 taaagagtta cgcaacctgc aagaagaaga catcaaagtg gtgctggcga ttgccaacga
     4801 ttcccatcta atggccgatc tgccgtggat tgcagagtct attcagctac ggaatattta
     4861 caccgacccg ctgaacgtat tgcaggccga gttactgcac cgctcccgcc aggcagaaaa
     4921 agaaggccag gaaccggatc ctcgcgtcga acaagcgtta atggtcacta ttgccgggat
     4981 tgcggcaggt atgcgtaata ccggctaatc ttcctcttct gcaaaccctc gtgcttttgc
     5041 gcgagggttt tctgaaatac ttctgttcta acaccctcgt tttcaatata tttctgtctg
     5101 cattttattc aaattctgaa tataccttca gatatcctta aggaattgtc gttacattcg
     5161 gcgatatttt tttcaagaca ggttcttact atgcattcca cagaagtcca ggctaaaccc
     5221 ctttttagct ggaaagccct gggttgggca ctgctctact tttggttttt ctctactctg
     5281 ctacaggcca ttatttacat cagtggttat agtggcacca acggcattcg cgactcgctg
     5341 ttattcagct cgctgtggtt gatcccggta ttcctctttc cgaagcggat taaaattatt
     5401 gccgcagtaa tcggcgtggt gctatgggcg gcctctctgg cggcgctatg ctactacgtc
     5461 atctacgggc aggagttctc gcagagcgtt ctgtttgtga tgttcgaaac caacaccaac
     5521 gaagccagcg agtatttaag ccagtatttc agcctgaaaa ttgtgcttat cgcgctggcc
     5581 tatacggcaa tggcagttct gctgtggaca cgtctgcgcc cggtctatat tccaaagccg
     5641 tggcgttata ttgtctcttt tgccctgctt tatggcttga ttctgcatcc gatcgccatg
     5701 aatacgttta tcaaaaacaa gccgtttgag aaaacgttgg ataacctggc ctcgcgtatg
     5761 gagcctgccg caccgtggca attcctgacc ggctattatc agtatcgtca gcaactaaac
     5821 tcgctaacaa agctactgaa tgaaaataat gccttgccgc cgctggctaa tttcaaagat
     5881 gaatcgggta acgaaccgcg cactttagtg ctggtgattg gcgagtcgac ccagcgcgga
     5941 cgcatgagtc tgtacggtta tccgcgtgaa accacgccgg agctggatgc gctgcataaa
     6001 accgatccga atctgaccgt gtttaataac gtagttacgt ctcgtccgta caccattgaa
     6061 atcctgcaac aggcgctgac ctttgccaat gaaaagaacc cggatctgta tctgacgcag
     6121 ccgtcgctga tgaacatgat gaaacaggcg ggttataaaa ccttctggat caccaaccag
     6181 cagacgatga ccgcccgcaa taccatgctg acggtatttt cgcgccagac cgacaagcag
     6241 tactacatga accagcaacg tacgcagagt gcgcgtgaat acgacaccaa cgtgctgaag
     6301 ccgttccagg atgtgctgaa tgaccctgcg ccgaagaaac tgatcatcgt tcatctgctg
     6361 ggtacgcata tcaaatacaa ataccgctac ccggaaaatc agggcaagtt tgatggcaat
     6421 accgatcatg ttccgccagg attaagcgca gaagagctgg aatcatataa cgattatgac
     6481 aacgctaacc tgtataacga tcatgtggtt gccagcctga ttaaagactt taaagcggca
     6541 gacccgaacg gattcctggt ttacttctct gaccacggtg aagaggttta cgacacgccg
     6601 ccgcacaaaa ctcaggggcg taacgaagac aacccgacgc gccacatgta caccattccg
     6661 ttcctgctgt ggacgtcgga aaaatggcaa gcgactcatc cccgtgattt ctcacaggat
     6721 gtcgatcgta aatacagcct ggcggaactg atccacacct ggtcagattt ggcgggctta
     6781 tcttacgacg gttacgatcc aacccgttca gtggtgaatc cgcagttcaa agaaactacc
     6841 cgctggattg gtaacccgta caagaaaaac gcgctgatcg attacgacac gctgccgtat
     6901 ggcgatcagg tgggtaatca gtaaatgacc tttgtatcgc gtctggcgct atttgccgga
     6961 tgcgatactg ccgcaatgtt tacatcgaac gcccatcagg ccacactttt caggtggcct
     7021 tttttcataa tgccagttac tgttttcccg actacatacc taagggaata acatgtatca
     7081 cgacgtcagc tatctgctct cccgcctgat caacggcccg ctgtccctgc gccagattta
     7141 ctttgccagc agcaacggcc cggtgcccga tcttgcgtat caggtcgatt ttccacggct
     7201 ggaaattgtg ctggaaggtg agtttgttga taccggcgct ggagcaacgt tagttcccgg
     7261 cgatgtgctg tacgttgccg ctggtggctg gaatttccca caatggaaaa cacccgctac
     7321 cacctttagc gtgctgtttg gcaaacagca actcggcttc agcgtcgtgc aatgggatgg
     7381 caaacaatat caaaatctgg cgaagcaaca cgtcgcccga cgcggcccac gtataggttc
     7441 ttttctgcta caaacgctca atgaaatgca aatgcagccg caggagcagc aaacggcaag
     7501 gcttatcgta gccagcctgc ttagccactg ccgcgatttg ctcggcagcc aaatccagac
     7561 cgcctcacgc agccaggcac tatttgaagc tattcgcgat tatatcgacg aacgctatgc
     7621 ctccgcgctt acccgcgaat ctgtcgcaca ggcattctat atttcaccaa actatctctc
     7681 gcacctgttt caaaaaacgg gggccattgg ttttaacgaa tacctgaatc acacgcgact
     7741 ggagcacgct aagacgttat tgaaaggcta tgacctgaag gtaaaagaag tggcgcacgc
     7801 ctgcggcttt gtcgacagca actacttctg ccgtctgttt cgtaaaaaca ccgaacgctc
     7861 gccgtcggaa tatcgccggc agtaccacag ccaactgaca gaaaaaccga ctactccaga
     7921 ataagatggg tttgctgcgg cgcagaaagc actttgcgca ccgcgctcat tacccgctgc
     7981 ggctcacgca ggaatgcgta gatgctgcat tgcacatagc ggcaatgttc aaatcgctcg
     8041 gcaccggcaa gctcgatatc cgtaatcaac agagcaacat ccgcccgacg gatatcctcg
     8101 tccgctaaac gattctccgt tcccagcgcg ccctgagttt caatgctgac gccccacttc
     8161 tctaactggc acaacttttc cagccgttcc gccgccatat aagtatgcgc cacgccgctt
     8221 acgcaggcgg ttactgccac caggtatgcc attttaacct cccacggtaa cctgaaatcc
     8281 ggcccgttct gccatttcgc gcatcgttgt cacatcggct gacgacggcg caggcacctc
     8341 tttcatcgac catgttttcc ccagcaggcg gtatttcggt tcgccgtact gatgaaacgg
     8401 taacagatgg atctgcctga ttttcagcgg gatcagtaca tccagcgcct gctgcatatt
     8461 ctcccggctg agcgtgaaac cagggatcaa cggtaaacgc gggatcacgt tgacaccctc
     8521 actcaccagc aaacgcagat tctccagcac gcgtggcagg ttcatcttca ccacatcccg
     8581 cgcctgagtc gcgtccataa tttttaaatc gaacagcact tcatcgcaca atttcgccag
     8641 tggtaatagc ttgctggctg gcgcgtctcc ggcagtttca atggcgcatg acacgcccca
     8701 caaccgcagc cgctgtaaaa aacgggtagc aaactccgcc tgcattaaca cttcgccgcc
     8761 agaaagcgtg acgccgccgc cggacgtgcg aaagaaaatg tcatctttca tcacttcccg
     8821 ttccagcgcg tcaaggctga tatcgcggcc aatccgttca aacgccccgg aggggcattc
     8881 atccgcatca cgcaaacatt tcgcacagtg cagacatttc gcctctctgc gtaccgtctg
     8941 gattttgccg gagatcgact ccggattagc acaccacggg caaagatgcg gacagccttt
     9001 aaaaaagacc accgtacgaa tgccctcgcc gtcattcagt gaataacgct gaatgttgaa
     9061 aatgcgcgcc acatcatcgc ggcgcgtttc cacaacgtta cagctgatgc gctgtccggc
     9121 ggatgatgtc atcctggatc tccttcgaca gttcgacaaa gaaggcgctg tatccggcaa
     9181 cgcgcaccac cagcccggca taatcttgtg ggcgctgttg cgcttcccgc aacgtgtcgg
     9241 cgttcaccac gttaaactga atatgctgca acttaagctg ggtaaacgcc cgtaagaagt
     9301 cggccagttt gcgtaatccc gcttcacctt ccagggtcgc cggagtgaat ttcacgttca
     9361 gcaacgtacc gttagacagc agcgtgttat cgagcttact gactgacttc agtaccgctg
     9421 ttggcccttg tgcgtcctgg cccagcatag gtgacaagcc gccgtctgcc agttgttccc
     9481 cggcaaaacg accgtctggc gtcgcgccaa ccaccgatcc caacgggacg tgagcagaaa
     9541 cggtatacga tcccggcgtg aagtaaccgc cgcgcgggtt ctggtatttt tccacttctt
     9601 tgcagtagtg gcgtaacagt tcggcgctga tgttatccac ctcgtcgata tcgttaccgt
     9661 atttctcgaa gcggttaatt aagcgagcac ggactttttc gccttctggc gttgcaaagt
     9721 tggcttttaa taccgacagc aattcgtcaa aacttaaacg ctgttgctca aaaaccatcc
     9781 ctttcaacgc atggagagaa tcgctcaggt tggcgatacc gatcccctgt acgccggaga
     9841 agttataacg cgcgccgcca tcggtaatat cgcggccttt ttccagacaa tcgctgataa
     9901 aagacgagag cagcggtaca ggtgcccagt cgcgatggcc gatatcacaa atattgctgc
     9961 cctcaaccat cagggtgatg tagtggctga tcttggcgcg gatctgctcc agtaaacctt
    10021 cataagtcag cgcagcattg ccttcatttt catacaggca gatttccatc actttcagca
    10081 gattaaacat cgcgatgtca tgcaagccgt aggttctgcc gggaatagat aattccacac
    10141 agcccactac ggaatagtcg cgcgcatctt ccagcgaaac gccacggttg aggaacgctg
    10201 gcaccaccac ttcatcgtta aagatttgcg gaataccggt gccgaggcga atggtttcgg
    10261 cggttttcat caggaacggc gtgtcgatca aggcgttagt gcgcacgccg aggttcggtt
    10321 gcggtaattg cacgctttga taggcgtcaa ggcaaaggaa cgaaagcacg ttcaccgcgc
    10381 tacgtccgtt ctcggttaac ccgccgagca gtgcggtata gccggtcggg aaaccggcga
    10441 aataacgcgc gctactggtg gagcgcaaca gcacgatgtc gttgcatttc acccataaag
    10501 attcgagtag ttctttcagg aacgccggat cttcgccctg ggttaaagat gcctgataga
    10561 acggcaacat atactggtcg aagcgcccca acgatagcga actggcgttg gattcgtatt
    10621 gcagaatgat gttcatgtac cagaataact ggcacgcctg ccagaacgtc tgcggcttat
    10681 gttcggcgtt atggcgggag atctccgcaa tagtcagcag ttcttcgcga cgctgggcat
    10741 ctgtgcagtt tgccgccatt gtttccgcca gttcagcgta acgcagaatg tgtttctgcg
    10801 aggcttccag cagtaacagc gcggcctgat aaaagtgatt ctccggctgt tgctgacaat
    10861 gttgctgcat ctgcgccacc agctccccca gcccgtgatt cagcaggcgt gggtaatcaa
    10921 taataatgtg cccctggcct ttatctgtct ggttgatgct gaaaatctgc gtgctggtcg
    10981 cggcttttac ttcatctgtc atctgcccgt tgatgaaatc tttcatcgaa cgtttttccc
    11041 agtacgggaa caactcttca cggtagatac gtttatcttc ttcactgata gcaaagcggt
    11101 cctgcggacg cgtcgggaat tgatccagct ctttcagcag ccagtaaggg tccatttccg
    11161 gcgacataat cccagcgcgc ggttttacgg tgcggttacc ggcaatcagt tcttcatcac
    11221 gaatcgaaat ttcaacatgt tcaaggatat acgctgttgc tttcgcccgg cgcaatatca
    11281 ccggttcgcc ttcggtttgc cgatggctgg cggtataaag cagcgcccgc tccagcgaga
    11341 tttcacgggt attggcaaac agtgcagttt tgaggcgaga gatacgattc gtcatgagga
    11401 actcctgtgg gaaagcgaac ccgcccttgc gagcgggaga agaacttaag cggtttgcgc
    11461 caggtgagct tcaattttgc tcataatggc gtcagcacgt tttactgcat cgctgatgtt
    11521 gacgcgcacg atggtcttgc cagcaaaacg ctcttcaaac ttgatgccga tatctttggt
    11581 cagaatcacc atatcagcgc tcgccacatc ttccgcagtc agttcatttt ccagaccaat
    11641 tgacccctgg gtttctactt tcacttccca gcctttcgct ttcgcggcac tttccagcgc
    11701 ctctgctgcc atataggtat gggcaacacc tgaaggacat gcggttactg caataatttt
    11761 cgtcatggct cgttcctcaa ttaattaatt tcaaaatcca aatccaggtc gtcttctttt
    11821 tcatcagtcg aagacccatt tttacgcgcc agacttttca gcacgttcac acaaacagcc
    11881 gtcaccactg ctccgacagc cactgctgcg atataaccca gcttgccttc aaccaccggc
    11941 agcacaatca gaccacccca gcctgcatag cactgcgcac cgaccagcgc cgcagttact
    12001 gcaccacaaa ctgaaccgac catgatggaa ggaatgacac gcagcggatc ggcagcggcg
    12061 aaaggaatcg ccccttcagt aacgccaaca cagcccatca ccagtgccgc tttgccggtt
    12121 tcgcgctctt ctgcggagaa atttttacga ccaatcagcg tcgccagccc catccccagc
    12181 ggcgggatac aaatacccac ggcagcgatg gccaccacgg tataaacacc ctgagcaacg
    12241 caaatcagca tgaaggcata ggccactttg ttaaccggac cgcccatatc gaacgccagc
    12301 atcagaccca tgatcaccgc cagcataaca atgctgccct gctgcatccc ctgaagccac
    12361 tgagtcaggc tgttggtcaa cgcccctaca ggctcgccca agccccacat catgatgcct
    12421 gcggtaatca gtgtgccgac gataggaata atgaagatag gcatcaccga gcgcagaact
    12481 ttatgcaccg gaattttctt caggtaatgc accacgatgc cgccgataat cccggcgatc
    12541 agtgcaccaa agaagcccgc accaaagctg ttaccaaccc aggcaccgat agcgcacgga
    12601 gccagcgcag aacgctctgc aatggaataa ccgatgtacg ctgcgaggaa aggcaccatc
    12661 agcgtcaagc ccgcaacgcc gatatcaaac agttttttca gatttggatc ggctacggca
    12721 tccggcactg cgcctttgcc atacaacatg acggaaaccg ccagcaaaat accgcccgat
    12781 accacgaagg gaatcatgtg tgaaacgccc gtcattaaat gctgacgggt atttttcagg
    12841 atctgcacca actcattcat aagctgctcc cgggcttgcg aaagcccatg tccatatttt
    12901 tgatggagga aacaataggc aatcgcaggc ggagcaaaca gtggataaaa aggccattta
    12961 ctggactttt gtaatatcag tacaaaaatg cgatccgcct cataacttgc gataaaccgg
    13021 aagcatgccg caaaacagcg tcttgtgaga cgcgtcgcaa attgacggtt acattacatt
    13081 tgtccagctt ttggcagatt tgtcacatgg caggccgttg ccgtgcgctc ttatcctgta
    13141 aacagaaaac cgtaccggga gagaggcaat ggccctgatt gtggaattta tttgtgagct
    13201 acctaacggc gtacatgcac gtccggcaag ccacgttgaa acgctgtgta atactttttc
    13261 atcacaaatt gagtggcata acctgcgcac tgaccgcaag ggcaacgcca aaagcgccct
    13321 tgcgctgatt ggcaccgata cgctggcggg cgataactgc cagttactga tttccggggc
    13381 cgacgaacag gaagcgcacc agcgtttaag ccaatggctg cgcgatgaat tcccccactg
    13441 cgatgcgccg ctggcggaag ttaaatctga cgaactggaa ccactgccgg tttcactgac
    13501 caatctgaat ccgcaaatta tccgcgcccg caccgtgtgc agcggtagtg caggcggcat
    13561 tctgacgccg atctcttctt tagatctcaa tgcgctgggt aatcttcccg cagccaaagg
    13621 cgttgacgcc gagcaatccg cactggaaaa cggcctgacg ctggtactga aaaacattga
    13681 gtttcgtctg ctggatagcg acggtgctac cagcgcgatt ctggaagctc accgatccct
    13741 ggctggcgat acttccctgc gcgaacattt actggcaggc gtcagcgccg gattaagctg
    13801 cgccgaagca attgttgcca gcgcgaatca cttttgcgaa gagttttccc gttccagcag
    13861 cagctacctg caagaacgtg ccctggacgt acgcgacgtc tgcttccagt tactccagca
    13921 aatctacggt gagcaacgct tcccggcacc gggaaaactg acgcagcccg ccatttgtat
    13981 ggctgatgaa ctgaccccca gccagttcct cgaactggat aaaaatcacc tcaaaggatt
    14041 gttgctcaaa agcggcggca ccacctcaca tacggtgatc cttgcccgtt cgttcaacat
    14101 tccaacgctg gttggtgtgg atattgatgc ccttactccg tggcagcaac aaacgattta
    14161 tatcgacggc aacgccgggg cgattgtggt tgagccaggg gaagccgtag ctcgttatta
    14221 tcagcaagaa gcccgcgtac aggacgccct gcgtgagcaa cagcgtgtct ggctgaccca
    14281 acaagcccgt accgctgacg gtatccgcat tgaaattgcc gctaacatcg ctcactccgt
    14341 ggaagcgcag gccgcattcg gcaatggtgc ggaaggcgtt ggtttgttcc gcactgaaat
    14401 gctctatatg gatcgcacca gcgcaccggg cgaaagcgag ttgtacaaca ttttttgtca
    14461 ggcgctggaa tccgccaacg gacgcagcat tattgtgcgc actatggaca ttggcggcga
    14521 caaacccgtt gattatctga acattcccgc agaggcaaac ccgttcctcg gttatcgcgc
    14581 cgtgcgtatt tatgaagagt acgcgtcgtt gtttaccacg cagctacggt cgatcctccg
    14641 cgcctccgct cacggcagcc tgaaaatcat gatcccgatg atctcctcaa tggaagagat
    14701 cttatgggtg aaagaaaaac tggcagaagc caaacagcaa ctacgtaacg aacacattcc
    14761 gtttgatgag aagatccagc tcggcattat gctggaagtg ccgtcggtga tgttcatcat
    14821 cgatcaatgc tgcgaagaga ttgatttctt tagtattggt agtaatgacc tgacgcaata
    14881 tctgctggca gtggatcgcg ataacgctaa ggttactcgt cactacaaca gcctgaatcc
    14941 ggccttcttg cgggcgctcg attacgccgt gcaagcggtg catcgccagg gcaaatggat
    15001 tggtctgtgc ggtgagctgg gagcgaaagg ttccgtgctg ccgttgctgg tcggcttagg
    15061 gctggatgaa ctcagcatga gcgcaccatc aattccggcg gcgaaagcgc ggatggcgca
    15121 acttgatagc cgtgagtgcc gcaagttgct caaccaggca atggcctgcc gtacttcgct
    15181 ggaagtggaa cacctgctgg cgcaattccg catgacccaa caggacgcac cgctggtcac
    15241 cgccgagtgc atcacgctgg aaagtgactg gcgcagcaaa gaagaagtgc tcaaaggcat
    15301 gaccgataac ctgctgctgg cgggccgctg ccgctatccg cgtaaactgg aagccgactt
    15361 atgggcgcgc gaggccgttt tctctaccgg tctgggcttt agttttgcca ttccacacag
    15421 caaatcagaa cacattgagc aatccaccat cagcgtggcg cgtctgcaag cgccggtgcg
    15481 ctggggcgat gatgaagcgc aattcatcat tatgttaacc ctgaacaaac acgctgcggg
    15541 cgatcagcac atgcgcattt tctcgcgcct cgctcgccgc atcatgcacg aagaattccg
    15601 taacgcgctg gttaacgccg cctctgccga cgctatcgcc agcctgctgc aacatgaact
    15661 ggaactgtaa aaggaaacat catggaactg tatctggaca ccgctaacgt cgcagaagtc
    15721 gaacgtctgg cacgcatatt ccccattgcc ggggtgacaa ctaacccgag cattatcgct
    15781 gccagcaagg agtccatatg ggaagttctg ccgcgtctgc aaaaagcgat tggtgatgag
    15841 ggcattctgt ttgctcagac tatgagtcgt gacgcgcagg gtatggtgga agaagcgaag
    15901 cgcctgcgcg acgccattcc gggcattgtg gtgaaaatcc cggtgacttc cgaaggtctg
    15961 gccgccatta agatgctgaa aaaagagggt attactacgc ttggcaccgc agtgtacagt
    16021 gctgcacaag ggttactggc cgcgctggca ggggcaaaat acgttgctcc gtatgttaac
    16081 cgcgtagatg cccagggcgg ggacggcatt cgtacggttc aggagctgca agcgctgtta
    16141 gaaatgcacg caccagaaag catggtgctg gcggccagct ttaaaacgcc gcgtcaggcg
    16201 ctggactgtt tactggcagg atgtgaatcc atcaccctgc ccttagatgt agcgcaacaa
    16261 atgctcaaca cccctgcggt agagtcagct atagagaagt tcgaacacga ctggaatgcc
    16321 gcatttggca ctactcatct ctaaaggagc aattatggac cgcattattc aatcaccggg
    16381 taaatacatc cagggcgctg atgtgattaa tcgtctgggc gaatacctga agccgctggc
    16441 agaacgctgg ttagtggtgg gtgacaaatt tgttttaggt tttgctcaat ccactgtcga
    16501 gaaaagcttt aaagatgctg gactggtagt agaaattgcg ccgtttggcg gtgaatgttc
    16561 gcaaaatgag atcgaccgtc tgcgtggcat cgcggagact gcgcagtgtg gcgcaattct
    16621 cggtatcggt ggcggaaaaa ccctcgatac tgccaaagca ctggcacatt tcatgggtgt
    16681 tccggtagcg atcgcaccga ctatcgcctc taccgatgca ccgtgcagcg cattgtctgt
    16741 tatctacacc gatgagggtg agtttgaccg ctatctgctg ttgccaaata acccgaatat
    16801 ggtcattgtc gacaccaaaa tcgtcgcggg cgcacctgca cgtctgttag cagcgggtat
    16861 cggcgatgcg ctggcaacct ggtttgaagc gcgtgcttgc tctcgtagcg gcgcgaccac
    16921 catggcgggc ggcaagtgca cccaggcggc gctggcactg gctgaactgt gctacaacac
    16981 cctgctggaa gaaggcgaaa aagcgatgct tgccgccgaa cagcatgtag taaccccggc
    17041 gctggagcgc gtgattgaag cgaacaccta tttgagcggc gttggttttg aaagcggcgg
    17101 gctggcggcg gctcacgcgg tgcataacgg cctgaccgct atcccggacg cgcatcacta
    17161 ttatcacggt gaaaaagtgg catttggtac gctgacgcag ctggttctgg aaaacgcacc
    17221 ggttgaggaa atcgaaaccg tagctgcact tagccatgcg gtaggtttgc caataactct
    17281 cgctcaactg gatattaaag aagatgtccc ggcgaaaatg cgaattgtgg cagaagcggc
    17341 atgtgcagaa ggtgaaacca ttcacaacat gcctggcggc gcgacgccag atcaggttta
    17401 cgccgctctg ctggtagccg accagtacgg tcagcgtttc ctgcaagagt gggaataacc
    17461 tactccaaac tcccggcttg tccgggagtt tgaacgcaaa attgcctgat gcgctacgtt
    17521 tatcaagcct acgaaatctc tgcaatatat tgaatatgcg tgcttttgta ggccggataa
    17581 ggcgttcact ccgcatccgg cactctgtgc cagtaatctg aaactcccac gttgttcagg
    17641 agttttttat ccgcttctgg acattttctc cacagaacaa tcgttagccc ggaaggtcga
    17701 atctgtgact cttattttca cggacgaacc agatgaaagc ttcactggcg ctgctcagcc
    17761 tgctaacggc atttaccagc cactccttaa aatctcccgc cgttccacca accgtggtac
    17821 aaatccaggc caataccaac cttgccatcg cagacggtgc cagacagcaa atcggcagca
    17881 cgctatttta cgatccggcg tatatgcagc ttacctatcc tggcggtgat gtcccgcaag
    17941 aacgcggggt atgttccgat gtggtgatcc gtgcattgcg cagccagaag gtcgatttgc
    18001 agaaactggt gcacgaagat atggcgaaga attttgccga gtacccgcaa aaatggcagt
    18061 taaagcgccc ggacagcaac atcgatcacc gtcgggtgcc taatctggaa acctggttta
    18121 cccgccacga taaaacacgc cccatcagca agaaccccag tgactatcaa gcgggcgata
    18181 ttgtctcctg gcgactggat aacggactgg cacatattgg ggtggtgtca gatggcttcg
    18241 cccgcgacgg tacgccgctg gtgatacata acattggcgc aggcgcgcag gaagaagatg
    18301 tgctgtttag ctggcggatg gtggggcatt atcgttattt tgttaaataa gcgatcgccc
    18361 cacatgaaga aaaaattcag atgcttttta cactgacagc ttcttttttc ttacggctca
    18421 ccagcgccag tgccagcaca atcagcacaa taccgctacc ttcaacggcc cccggattct
    18481 caccgagcag ccaccaggaa aagagtacgc cgcaaacggg aacggccagc gtgcttaagc
    18541 tggcaatact ggcaggcaag tttttcaata caaacaacca taagctccac gccagcgccg
    18601 tcgccagaat cgcactgtag gccagcgccc agaacacggt gggctgccag tcaatttcac
    18661 gttgcggcac cagtaaagcg accacactca tcaccagcgc cgcatagagc atctgccagg
    18721 atgttaacga taataaatcc acgcgcggat gacgggtgta taggcgttta gcaacaatcg
    18781 cgctcgcccc ccaactgacg ccggagagga ttgccagcat ggcacttttc attgaagaga
    18841 aatcgagttg ccacggctgc aacaccaaaa ataagccgaa agcggcaatc agtatcgcga
    18901 aatattgccc acgtcgcagg cgttcaccga gaaacaacgc ggcgaaaatc accacccaga
    18961 acggcatggt atagcttagg atcgccacct tccccgctcc gccgctgacc agcgcccact
    19021 gcgccaggcc aaccatcccg caggtttgta acagggcaat ggctaaggtg tatttaaacg
    19081 gtgtcgggcg cattccgcga ccacgtaata aaaggacgat gaataaaacg agagcgccga
    19141 aaatgcagcg taaggcggta aagtcgaagg caccgatgta gctggtgact tgcttcatga
    19201 aaatccagct ataactccag ataagtgtga gcacaaccag gccactgatt gccagtgggt
    19261 tgctctttcc tgcggcagac atgagagatc cagtgtgtag ttttcctgtg gacactatac
    19321 gccggtaaag aaacttcagc gactgcctga aagacagtcg ctgaagaagg tcagattaca
    19381 gcaggtcgaa acggtcgagg ttcatcactt tcacccatgc cgccacgaag tctttaacaa
    19441 acttctcgtg ggcatcgctg ctggcgtaaa cttccgctac cgcacgcagg acagagttag
    19501 aaccaaatac cagatccgca cggctggctg tgtatttcac ttcgccggtt tcacggtcac
    19561 gaccttcgaa cagctctttc gattcgtcag tcgctttcca ctcgtaacgc atatccagca
    19621 agttcacgaa gaagtcattg ctcaatacgc caacgcggtc agtgaagacg ccgtttttgc
    19681 tgccatcgaa gttggcaccc agtacacgca tcccgcccac cagcgcagtc atttccggcg
    19741 cggtcagcgt cagttgctgt gctttgtcga tcagcagtga ctcggtggtg gaaacatcca
    19801 gacgagcgcg atagttacgg aaaccgtcag caattggctc cagcagctcg aacatctcaa
    19861 tgtcagtctg atcctgacgc gcatcaacgc ggcccggcgc aaacggtaca tgaatgctca
    19921 aacctgcggc gcttgcggct ttctcaacac caaccacacc agccagcacg atgatatccg
    19981 ccagcgaggc tttaccagac tctttctgga ttttctccag aacaggcaga gcacgaacgg
    20041 ctgcggcgtt cacatcccag tcgcgctgcg gcattaatgc cagacgcgca ccgttggcac
    20101 caccgcgttt gtcgccacca cggaaggtag aagcagaagc ccaggccacc gataccagct
    20161 cactaacaga cagaccagaa tccgcaatcg cgaatttcag atcgataatg tcctgctcgg
    20221 tcgggttgta gatcggctgc ggcagcggat cttgccagat cagatcttct ttcggcactt
    20281 ccggcccgat gtagcgagat ttcggcccca tatccctgtg cgtcagtttg aaccaggcac
    20341 gggcaaaggc ttcgttgaac gcctgcggat cgttgaggaa acgacgagag atcttctcga
    20401 actcaggatc aaaacgcagc gtcaggtcgg tcaccagcat tgtcggttta cgtttcttcg
    20461 acggatcgaa cggatccggg ataatttccg gtgcgtctac cgcttcgaac tggattgctc
    20521 cagccggact gcgggtctgt acccactcat acttgaacag attctcgaag aaatagttgc
    20581 tccactgggt cggcgtctgg gtccagacta cttccagacc agaggtaatg gcatctgcgc
    20641 caacgccgct gccgtaagtg ctcgcccagc ctaaaccttg ttcttcaatc ggtgcagctt
    20701 ctggatcagg acctacgttt gatgtcggac cggcaccgtg ggttttaccc agcgtatgac
    20761 cacccgcaat cagcgccacg gtttcttcgt cgttcatgcc catgttgccg aaggtcgcgc
    20821 ggatagctgc tgccgcagaa agcggttcgc cgctgtgatc cgggccttcc gggttaacgt
    20881 aaatcaggcc catctcggtt gcacccagcg gtgctttcgc cagcgcttcc ggatgacggt
    20941 gagtcagcca ggctttttca tcaccccagt taacatccag atccggttcc cagacgtctt
    21001 cacgaccggc accaaaaccg aaggtacgga agccggagtt ttccagcgcc acgttacctg
    21061 cgaggataaa caggtcggcc caggagattt tctgaccata tttctgtttg attggccaca
    21121 acaggcgacg cgctttatcg aggcttacgt tatccggcca ggagttcagc ggtgcaaaac
    21181 gttgctgacc acgacccgcg ccgccgcgtc catcgattga acggtaagta cctgcgccgt
    21241 gccaggccat acgaataaac agaccggcgt agctgcccca gtcggctggc caccacggct
    21301 gagattctgt cagcagggct ttcagatctt ttttcaggcc gtagtaatct aatttgctga
    21361 attctttgcg gtagtcaaag tcctcaccca gcgggttaga acgattagaa tgttggttta
    21421 acaggtcaac acggagttga tttggccacc agtcgcgagt ggttgtgccc gcccccgcgc
    21481 tctggtcgtg accgccctga tggaacgggc atttgccagt ggctgtcgtg ttatggatat
    21541 cgtctgacgt gctcatcaat gtgctcccct ctacagtgtt accgttacga tacacagcgt
    21601 tagagagaag ttataattga attaatccac ggatgcgata gttgagatct tacatattgt
    21661 tggttaaaga gatgtaggtc aaattgatcg taattaagct tttttgatta tgtccgaatt
    21721 cggacaaatg ctttataaaa aagggtttta gcttcatatc cttcaagtaa tgagggagaa
    21781 aacaaggctg acaaccttat tttcccgccc tcatttcgag gcagcatttt gtgctctgtt
    21841 taaaatttgt gatcactgtg tgattttcac aaaagccaca ctatttataa accaggtcga
    21901 acacccagcg tatggcaaat cgcgtaactc atttcagcgc ggttaagcgt atagaagtgg
    21961 aaatctttca ctccttcacg gcttaaaatc ttcaccatat ccatggcaat attcgctccc
    22021 accagtttgc gggtttcggc atcgtcgtcc agcccgtcaa acatttgtgc catccacgcc
    22081 ggaatacgca cgttggtcat atcggcaaat ttcttcgctt gtttaaagtt cgagaccggc
    22141 aaaattcccg gaataatttc cacatcaata cccgccgata cgcagcggtc acgaaaacgc
    22201 aggtagcttt cgacatcaaa gaagaactga gtaatcgcgc ggttggctcc ggcatcgact
    22261 ttgcgtttca ggttgagcaa atccgcctga gcgctttttg cttccgggtg aacctccgga
    22321 tacgccgcca cggagatatc gaaatctgcc acttctttta acagcgtcac caggtcagaa
    22381 gcatacattt ccggcttacc acttcccggc ggcagatcgc cacgcagcgc cacgatatga
    22441 cgaataccgt tattccagta gtcgcgtgca atggtgcgca gctcgtcggg cgtcgcatca
    22501 atgcaagtaa gatgcggtgc cgcttccaga ccagtgcgat ctttaatgcc tttaataatg
    22561 ctgtgcgtac ggtcgcgctc gccggagttc gcgccatagg tcaccgatac aaacttcggt
    22621 ttcaggctgc taaggcgatc gatggagttc cacagggtct gctccatttc actggtacgc
    22681 ggcgggaaaa actcgaacga aacgttaatc tgcccctgga cttctgccag gctctgattc
    22741 agggcatccc gctggctggc gtgaaaaaag ctcatacctt acctcatcaa tcgcttgtag
    22801 ttatatgttg tgttgtgcac atctatccgt ttagacgtcc agatgtaaag atgaaggaaa
    22861 agccgaaggg cgtcaaccga aaaatgacca atgatggtga ggaaaaatca gggaagatga
    22921 aaaaacttca ggatggtaag aaaaagaaaa tccggtatgc aaagcaaacc ggactgcaaa
    22981 tataaactaa ccatgtactt attgcttctt gccggattcg tcgtaaacgc ttaatccggc
    23041 ctaatcctat gataattcaa tatatttcat tagaaaccat aaacccgaaa acatgagtac
    23101 cgggcattat taaatttctg aaattacaac aactgtgcca gccggttgat atccgactga
    23161 atcgccccgg cggtgacgtc gcgccccgcg ccaggcccac ggatcaccag cgggttgtcg
    23221 cgataccagc ggctttcgat ggcaaagacg ttatcgcacg gcagcagtga tgccagcgga
    23281 tggtcttcac gcaccgcctc cacgcccaca cgcgctttgc cgttggcatc gaaacgcgcc
    23341 acgtagcgca gcaccagccc catttcgcgg gccgcttcca gccgttgcac catctgctcg
    23401 ttaagttcat cgccattttc aaagaaatgg tcgatgctgc cgccttcgca atgggcaggc
    23461 accagcgact ccacgcgtac ctgatccggt tcgatgttgt aacctgcttc acgcgccaga
    23521 atcaccagct tgcgcattac gtctttgccg gaaaggtcat cacgcgggtc aggctcggtt
    23581 aacccttgct gccacgcctg atccaccagc tcggtaaacg gcacgctacc gtcgaattgc
    23641 aggaacagcc aggagagcgt gccggagaag atcccactga tcgacaaaat agtatcgccg
    23701 ctgtcgatca gatcgcgcac ggtatggttg atcggcaagc ccgctccgac ggtagcattg
    23761 tacagccagt gacgcccggt tttttcgaag gcgtcgtgga tctggcgata tttattgctg
    23821 tcgctggctc ccgccagttt gttggcgctg atgacgtgga aaccgtggct ggcgaaatca
    23881 agatactgat cagcaagctg ctggctggcg gtaacgtcca gcaccactaa atcatcatac
    23941 ggatgggcgc gcatccacag gaacaacgac tcttcatcct gctcaaccgc ttcatcgttg
    24001 aagaaggcta acgcgcggct ggcgtccagc ccgtcatagc tcaacaggct gcggcggctg
    24061 tccaccacac ctgccagcac aaactcaaag ccggtacgtg ccgaaagcgt gctctgctca
    24121 cgggcgaaca gttccagcca acgggaaccg atattgccct taccgaacaa taccaggccg
    24181 atgcgttttt ctgcgcggaa gacggactga tgcagcccct gaatcaggct ttcggtcggg
    24241 ccggtgcgca gtactgccac caggctgatg ccgtcatcgg actgccaggt aaattcgacc
    24301 ggctggcctt tcagttgctg ccagaagcgg tggcaatgca gcgggttacg ggtgacgcct
    24361 gcaccgacca tcgccaccag cgccagcccc tgacgcaggc gcagttcgcc aggtaatccc
    24421 gcttcgtcga ggattttcag cgcactgtcg gccacttctg aggtgtagca aaattgcagc
    24481 aactggcgat cgttatgtac gccaaccgcc agcgggcgta cctgcgcgcg tttcaggatt
    24541 tggtcgatct ctttatgcgc cagtttgaaa tcctgactgg cgggcacctg aaactcaatc
    24601 aaacagacat catcgtggct ggtgacaata cgcgcaccag taccggaggc cagcacgcgt
    24661 tcaatgcgcg tggaaccttg atccggcgtg tagctacagc gcagttgcag gtcgatttcg
    24721 ctgccagaaa ccggctgtaa agtacgggcg tgaagaacgg gagccgccag gcgcgccagt
    24781 tcgctggcct catccagacg cagcaacggc agcaggcagg catctttcac tttacgcggg
    24841 tcggcactgt ataccccggc gacgtcgctc cagatggtta cgcgagaaac acccgccagc
    24901 gcaccgattt gtgtcgcgga atagtcggaa ccgttacgcc ccagcagcac cgtttcaccg
    24961 gcgttgttgc ggctgataaa tccggtcacc accagacgtt tgcccggatg ttgcaccagc
    25021 agctgttgca gcaacgggta agaaagccct tcatcaacct gcggttgtgc ggcgcgttca
    25081 gcgcgtaaaa actcgcgggc atcaagccag gccgctggca gcccttgttg attaagtacc
    25141 gcagacatca ggcgtgccga ccacacttca ccgtgcccca ccacttccgc atacactgcg
    25201 tcgttaatac cgctgtcgag cagcgccgcc aggcgctcaa ggtcgctgac aaaagcgcta
    25261 atgaggctat cggcttcttc agcgggtagc agaccgctaa tcagatcgca ctgatagcga
    25321 cgcagcgttt gttgaacctg atgcgcagag agacgatcgg tctggcttag tttcaaccag
    25381 ttaatcaact ggttagtggt gctaccggcg gcggaaacca ccatcatatc gtcaggctga
    25441 gagtactccg ccataatgcc cgcgacacgc aaataacact tcacatcagc cagactactg
    25501 ccaccaaatt tatgcagctg acgacctttc gcccctgcct gcgcaatcac actcattttt
    25561 accccttgtt tgcagcccgg aagccatttt ccaggtcggc aattaaatct tcgccatctt
    25621 caataccggt ggagatacgc agcagcgtct cggagatccc ggcggcagca cgcgcttctg
    25681 gtgccatgcc tgcatgtgtc atggttgcgg cgtgagagat taaactttcc actcccccta
    25741 atgattccgc cagcgtaaac aacgacagcc cgctcaggaa acgacgcagc gtctgctcat
    25801 cgccatccag ttcaaaactc aacattgcgc caaagccttt ttgctgacgc gcggcaattt
    25861 catgcccctg attttccggc agcgacgggt gatacagttt tttcaccaac ggctgggttt
    25921 gcagatattt cacaatcgcc tgcgcgttac gctgggccaa ctccatgcgc ggcaccagcg
    25981 ttcgcaaccc gcgtagcagc agatagctgt caaacgcgcc gcccgtcacg ccaatattgt
    26041 ttgcccacca ggcaagttca gtgacaacgt ccgggtcttt agcaatcacc acgccggcca
    26101 ccacgtctga gtgaccgttc agatatttcg tgcatgaatg caacaccaga tcggcaccta
    26161 atgccagcgg attttgtaat gccgggctta agaaggtgtt atccaccacg ctcaccgccc
    26221 cgacttccct tgccagatgg cagattttcg caatatccac gacgcgtaac aatggattac
    26281 ttgggctttc taccagtacc agtttgggtt tttctgccag cgctgcccgt aatgcctgtt
    26341 catcgccttg atcaacaaac aacacgcgat agcaaccgcg tttcgccagg ctgtcgaaca
    26401 ggcgatagct accgccgtag cagtcgtgcg gcgcaaccag cagatcgcca ggtttcaaaa
    26461 agacggtcgt taccaggtga atcgcggaca tgccggtatt agtaagtact gcaccagcac
    26521 cgccttccag ttctgccagc gcacgctgaa ccacatcgcg cgttgggttg ccgcgacgcg
    26581 agtaatcatg cgcgcgcggt tcattaaatc cggtaaagtt atatgtgctg gaaagatgga
    26641 tcggtgggac aacgcaacca tactgttcgt cgtcatttaa cccgctacgc actgcgatgg
    26701 tggcctgttt acgcgtcatg tgatgaagtt ccctgggctt tgtcggtgaa atgtcaggca
    26761 ccagagtaaa cattgtgtta atggacgtca atacatctgg acatctaaac ttctttgcgt
    26821 atagattgag caaatcccaa atagccgtta aaattatatg cattatcacg ccgacaggtg
    26881 cattacacga tgtcacggta acgcctgtac ggtaaactat gcgggtttac ggtcagtacc
    26941 cacataaatt gtgtggtctg gtctcaattt attgacgaag aggattaagt atctcatggc
    27001 tgaatggagc ggcgaatata tcagcccata cgctgagcac ggcaagaaga gtgaacaagt
    27061 caaaaagatt acggtttcca ttcctcttaa ggtgttaaaa atcctcaccg atgaacgcac
    27121 gcgtcgtcag gtgaacaacc tgcgtcacgc taccaacagc gagctgctgt gcgaagcgtt
    27181 tctgcatgcc tttaccgggc aacctttgcc ggatgatgcc gatctgcgta aagagcgcag
    27241 cgacgaaatc ccggaagcgg caaaagagat catgcgtgag atggggatta acccggagac
    27301 gtgggaatac taaagcggaa aagagcggcg cggagttaac cagccgcttt tttcatacaa
    27361 ggacgtttaa cgatgaaaat caggctactg atcctcagcc tgctggtttc tgtgcctgcc
    27421 tttgcctggc agccgcaaac tggcgacatc atctttcaga tctctcgctc atcgcaaagt
    27481 aaagcgatcc aactggcgac ccattccgat tacagccaca ccggtatgct ggtgatgcgt
    27541 aacaaaaagc cctacgtttt tgaagcggtc ggcccggtga aatacacccc gctcaagcag
    27601 tggattgccc atggtgaaaa gggcaaatac gtcgttcgcc gcgttgaagg cggactgagt
    27661 gttgaacaac agcaaaaact ggcgcaaacg gcaaaacgtt atctcggtaa accgtatgat
    27721 ttcagcttct catggagcga cgatcgccag tactgttcgg aagtggtgtg gaaggtttac
    27781 cagaacgcgc tgggaatgcg cgtgggcgaa cagcagaagt tgaaagagtt tgatctcagc
    27841 aacccgctgg tccaggcgaa gctcaaagaa cgctacggta aaaatattcc gcttgaggaa
    27901 acggtagttt caccacaagc cgttttcgac gcgccacaac tcaccacggt tgccaaagag
    27961 tggccgctgt tttcgtggta atggatgatg aaaaaaataa ttcccgccgt agtctgtttg
    28021 ttgctttgta gttgcacgac ctttccgcaa gctgtcgctc cggtaaacgg tgatgcctcc
    28081 aattagttga attaaggata taatactcgc ttttaaggtt gtttcatggt cacagttaac
    28141 cttcattgcc cccgttgcca gtccgttcag gtttaccgtc acgggcagaa cccaaaaaga
    28201 catgatcgat tccgcggccg tgactgtcac cgggtatttc agctcagtta ctgttacgag
    28261 ccccgtaaac cgggagttaa agaccaaatc gctgaaatga ctttcaacgg tgccggggtt
    28321 cgcgatactg cccgtacgct gaaaatcggc attaatacag tcatccgaac tttaaaaaac
    28381 tctcgccacg aagaataacg tcatccccgg tcgctcatgc tgatgtggca ctcatctgca
    28441 aacttagtca gcaatggagc tttgtcggca gtaaagcgcg acagcactgg ctctggtacg
    28501 tgtacaacac gaaaactggt ggtgttctgg cctacacttt tggcccccgg actgacgaaa
    28561 cctgtcgtga attgcgggcg ctgcttaccc ttttaccatc ggcatgttga ccactgacga
    28621 atggggtagt tacgctagag agttaccgaa ggagaagcat ctgactggta agatttttac
    28681 tcagcacatt gagcgtaaca atctgaccct gagaacccga atcaaacgtc tggcccgcag
    28741 aaccatctgt ttctcgcgtt ctgtagaact tcatgaaaaa gtcatcggtg cttttatcga
    28801 aaatatatgt tctactaatt ggagtcatca cccttctgat aagctttcct tggatgatta
    28861 ccagactgaa tacacagcca aagattttta cctcattctg ctcttttttc tcaaatgaca
    28921 tatattatat tttacatcat attcataggc atatgacatt cccccgcccc ccattatgtc
    28981 attgtgggtt gatgttgggc tttcataaaa aaaaacatct ttttttgaac tagcattaat
    29041 tatatttact ttatatatta aaatattttc tccatctgga aatattttag ccacacaata
    29101 taaagcagct gaatccgaat tacttaaaaa tatattatca tcacccataa ctccagccat
    29161 taaatgactg gagactaaag caaggaaata acaacattgt attgccttca ttttaattac
    29221 tcccttgcct gataattgta actccattcc ctttagcttt gggattatat tttagatagg
    29281 tgttatttgg tgttcccaag taactactgt attctttttt ttccattcca ttcatataag
    29341 aattcgttaa atctttgctt gaaaaattta gactatcata aacatcattt tcttttgtta
    29401 ctttatttcc tttatcgtca gagtaaaaac cgtgagtgtg ataatcgcct actctctctg
    29461 aaccttcagg gcattttata tttcgcaagt ctacactatc attatcattt gaacttatcg
    29521 gattcatggg gaaatattca ccttgcttct tacatattag tccaccatat tctttattct
    29581 cgttaattga ttctccatta cacattgcca atgcaaatat tccaatatca tctatatttt
    29641 ttgatttaaa ttcatataat cctaatggat ccatgctatt caccgggctc agcggatatg
    29701 tatacaggtt ccatcccccc ttcagcccaa tcggatcctg agtgatatac cgcccctgca
    29761 gcgggtcata atagcggtgg cggttgtaat acaggccgga ctcctcatca tactgctg
//
LOCUS       DAEVZA010000050        29632 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEVZA010000050 DAEVZA010000000
VERSION     DAEVZA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.050"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1471
                     /gene="rcsD"
                     /locus_tag="LAF92_002286"
     CDS             <1..1471
                     /gene="rcsD"
                     /locus_tag="LAF92_002286"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBI9791286.1"
                     /translation="EQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVN
                     KKLKQAQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLV
                     RLVDEIQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHD
                     MRRGDRDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHE
                     MDNLHFPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKML
                     AADPEVEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIF
                     LTDNPSNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVT
                     ESPLGGDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAML
                     NLVPGKQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            1488..2138
                     /gene="rcsB"
                     /locus_tag="LAF92_002287"
     CDS             1488..2138
                     /gene="rcsB"
                     /locus_tag="LAF92_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBI9791287.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(2338..5187)
                     /gene="rcsC"
                     /locus_tag="LAF92_002288"
     CDS             complement(2338..5187)
                     /gene="rcsC"
                     /locus_tag="LAF92_002288"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBI9791288.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(5462..6238)
                     /locus_tag="LAF92_002289"
     CDS             complement(5462..6238)
                     /locus_tag="LAF92_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBI9791289.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(6243..7892)
                     /locus_tag="LAF92_002290"
     CDS             complement(6243..7892)
                     /locus_tag="LAF92_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBI9791290.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWATGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(7893..12407)
                     /locus_tag="LAF92_002291"
     CDS             complement(7893..12407)
                     /locus_tag="LAF92_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBI9791291.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLGDA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(12431..13054)
                     /locus_tag="LAF92_002292"
     CDS             complement(12431..13054)
                     /locus_tag="LAF92_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBI9791292.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(13051..14739)
                     /locus_tag="LAF92_002293"
     CDS             complement(13051..14739)
                     /locus_tag="LAF92_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBI9791293.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDETLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(14888..17515)
                     /gene="gyrA"
                     /locus_tag="LAF92_002294"
     CDS             complement(14888..17515)
                     /gene="gyrA"
                     /locus_tag="LAF92_002294"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBI9791294.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            17662..18384
                     /locus_tag="LAF92_002295"
     CDS             17662..18384
                     /locus_tag="LAF92_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBI9791295.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(18512..22246)
                     /gene="yfaL"
                     /locus_tag="LAF92_002296"
     CDS             complement(18512..22246)
                     /gene="yfaL"
                     /locus_tag="LAF92_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBI9791296.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSVSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQMRLGLHSEWRTAVHVIPTLDLNYYHNPHSTEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            22942..25227
                     /gene="nrdA"
                     /locus_tag="LAF92_002297"
     CDS             22942..25227
                     /gene="nrdA"
                     /locus_tag="LAF92_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBI9791297.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            25316..26446
                     /gene="nrdB"
                     /locus_tag="LAF92_002298"
     CDS             25316..26446
                     /gene="nrdB"
                     /locus_tag="LAF92_002298"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBI9791298.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            26446..26700
                     /gene="yfaE"
                     /locus_tag="LAF92_002299"
     CDS             26446..26700
                     /gene="yfaE"
                     /locus_tag="LAF92_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBI9791299.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(26754..27404)
                     /gene="inaA"
                     /locus_tag="LAF92_002300"
     CDS             complement(26754..27404)
                     /gene="inaA"
                     /locus_tag="LAF92_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBI9791300.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            27586..27825
                     /locus_tag="LAF92_002301"
                     /pseudo
     CDS             27586..27825
                     /locus_tag="LAF92_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(27867..28943)
                     /gene="glpQ"
                     /locus_tag="LAF92_002302"
     CDS             complement(27867..28943)
                     /gene="glpQ"
                     /locus_tag="LAF92_002302"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBI9791301.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(28948..>29632)
                     /locus_tag="LAF92_002303"
     CDS             complement(28948..>29632)
                     /locus_tag="LAF92_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9791302.1"
                     /translation="DYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLR
                     YGILDWSPTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVF
                     FMTLVTIATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAA
                     GFTGLFGYLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHE
                     QLLQKRNGG"
BASE COUNT         7186 a         8042 c         7172 g         7232 t
ORIGIN      
        1 ggaacagcat caggggatta ttcaggcgac gatcaataac gagctgtatg agatccgcat
       61 gttccgcagc caggtcgcgc cgcgcacaca aattttcatt attcgcgatc aggatcgcga
      121 agtgctggta aacaagaaac tcaagcaggc gcagcgtctg tatgagaaaa accagcaggg
      181 ccggatgacc tttatgaaaa acattggcga tgcgctgaaa gaacctgcac agtccctggc
      241 ggagagcgcg gctaaactca acgccccgga aagcaaacaa ctggcgaatc aggcagatgt
      301 gctggtgcgg ctggttgatg aaatacagtt agcgaacatg cttgcggatg atagctggaa
      361 aagtgagacg gtgctgttct ccgtgcagga tttaattgat gaagttgtgc cttcagtgtt
      421 gcctgccatc aagcgtaaag gtctgcaact gctgattaac aatcatctga aagcacacga
      481 tatgcgccgc ggcgatcgcg atgccttacg acgtattttg ctgctactga tgcaatatgc
      541 cgtgacctca acgcaattgg gaaaaatcac ccttgaggtt gatcaggatg agtcctctga
      601 agaccgcctg acattccgca ttctggacac cggagaaggc gtaagtattc atgaaatgga
      661 taatttgcac ttcccgttta tcaaccagac ccaaaacgat cgctatggca aggcggaccc
      721 gctggcattc tggctgagcg atcaactggc gcgtaaactg ggcggtcatt taaacatcaa
      781 aacgcgggat gggcttggta cacgctactc tgtgcatatc aaaatgctcg ccgctgaccc
      841 ggaagttgaa gaggaagaag agcgtttact ggatgatgtc tgcgtaatgg tggatgttac
      901 ttcggcagaa attcggaata ttgtcactcg ccagttagaa aattggggtg caacctgtat
      961 cacacccgat gaaagattaa ttagtcaaga ttatgatatc tttttaacgg ataatccgtc
     1021 taatcttact gcctctggct tgcttttaag cgatgatgag tctggcgtac gggaaattgg
     1081 gcctggtcaa ttgtgcgtca acttcaatat gagcaacgct atgcaggaag cggtcttaca
     1141 attaattgaa gtgcaactgg cgcaggaaga ggtgacagaa tcgcctctgg gcggagatga
     1201 aaatgcgcaa ctccatgcca gcggctatta tgcgctcttt gtagacacag taccggatga
     1261 tgttaagagg ctgtatactg aagcagcaac cagtgacttt gctgcgttag cccaaacggc
     1321 tcatcgtctt aaaggcgtat ttgccatgct aaatctggta cccggcaagc agttatgtga
     1381 aacgctggaa catctgattc gtgagaagga tgttccagga atagaaaaat acatcagcga
     1441 cattgacagt tatgtcaaga gcttgctgta gcaaggtagc ctattacatg aacaatatga
     1501 acgtaattat tgccgatgac catccgatag tcttgttcgg tattcgcaaa tcacttgagc
     1561 aaattgagtg ggtgaatgtt gtcggcgaat ttgaagactc tacagcactg atcaacaacc
     1621 tgccgaaact ggatgcgcat gtgttgatta ccgatctctc catgcctggc gataagtacg
     1681 gcgatggcat taccttaatc aagtacatca agcgccattt cccaagcctg tcgatcattg
     1741 ttctgaccat gaacaacaac ccggcgattc ttagtgcggt attggatctg gatatcgaag
     1801 ggatcgtgct gaaacaaggt gcaccgaccg atctgccgaa agctctcgcc gcgctgcaga
     1861 aagggaagaa atttaccccg gaaagcgttt ctcgcctgtt ggaaaaaatc agtgctggtg
     1921 gttacggtga caagcgtctc tcgccaaaag agagtgaagt tctgcgcctg tttgcggaag
     1981 gcttcctggt gaccgagatc gctaaaaagc tgaaccgcag tattaaaacc atcagtagcc
     2041 agaagaaatc tgcgatgatg aagctgggtg tcgagaacga tatcgccctg ctgaattatc
     2101 tctcttctgt gaccttaagt ccggcagata aagactaatc atctgtaggc cagataagac
     2161 gcgttagtgt cttatctggc atttgcaccg attgccggat gcagcgtaaa cgccttatcc
     2221 ggcctacgat tcccattatt tcaacagatt acattaaagt aggccagata agacgcgtca
     2281 gcgtcgcatc tggcatttgc actgaatgcc agatgcggca taaacacctt atccgtccta
     2341 cgattcccgc gatttcctga ccctctcggc atataccgtc agcgtctgtt ttatcacatc
     2401 cagcgttacc ggcttcgaca ggcagctgtc cataccggac tccagacacc gctgcttctc
     2461 ttcagccaac gcattagcag ttactccgat taccggcaac gtcagtccca actgacgaat
     2521 gcgttgcgtc aagcggtaac catccatatt tggcatgttg acgtcgctaa gcacgatatc
     2581 aatatgattc ttgctaagta cattaagcgc atcgacacca tcattcgcgg ttttacattg
     2641 atagcccaac gatcccaact gatctgccag cagacgtcgg ttaatcggat gatcatccac
     2701 gaccagaatc atcatatcgt cattatcgct gaccgctttg tccgtcgacg gcagagcgtt
     2761 agcaggatcg tcgctctcca tctcgatcaa ataaatacgc gccaacaatg ccggtagctc
     2821 atgcggggca gccacactgt gtacccactc ccctggcgct ttctccagcg gaataccaat
     2881 atggcgacga cagaaggtca ctaccgctct accctgccat tttttactca ctacctcgtc
     2941 agtaattaac acatcttcgg gagtcggttc ctgcccttcg tatgttgtaa cgacgatgcc
     3001 gctgcgctgc aaactggttt ccaggaactg acagagcgac gcattgcgga ccgccagcca
     3061 gcagcgttta ccactcaacc cttccacgcc ttttttctgc gggtactgag cgccgtacaa
     3121 cggaatacgc acggtaaact ggctgcccat tcccggttct gaatctaccg agatatcgcc
     3181 gtccatcatg ctgatcagtt tttcacaaat cgccagaccc agaccggtcc cctggaagtt
     3241 tcgctgtacg cccgttccga cctggaagaa gggatcaaac aagcgcacca cttctttcgc
     3301 cggaatcccc acgccggtat cgcgaacacg gatagagaga taatcgccat cagcgcggac
     3361 atgcaaaact atacagccgg tatcggtgaa ttttatggcg ttactcaaca ggttggagat
     3421 gacctgctgt aaacgcatcg ggtcgccatt taaggccact ggcacatccg gttcaataaa
     3481 gcagtacaag cctaactgct tgcgtaccac cagcggtaaa tagttggcgg tgatgtggtt
     3541 catcacttca cgcggtgaaa actcacgcgg ttcgatcttc aactgttccg attcaatctt
     3601 cgagaaatcg agaatatcgc tgataatttt caacaacagg ctggaagagt tgttcattgc
     3661 cgtcaccagc cgatcgacgc ctttcggtaa ctctttggtt tgcaacagat ccaggttacc
     3721 gataatgcca tacagcggcg ttcgcagctc atgactgacg gtggcaagga acatcgattt
     3781 tgactggctc gcctgttccg ctgcttgtgc catctcctgc aacgactctt ccatcttcac
     3841 gcgcgaagaa acatccacca gcacacaaat ggccacgttt tcattacgat agcgcgaatg
     3901 gacgaagctg atttgcagat tggtattgtt gctggtcagg acatcaacaa aattgacctg
     3961 ctgcccacag ataatttgcg tcagtcgttg gcggtcctca tgcgtaagca tattgagata
     4021 ggtatgcgcc agttcgttac ttaaaatatt gacgccatca gcggtacgca aaatgcagat
     4081 acccactggc gcggaggcga caatcttgcg attgaactgc tcatgttctt ccagtcgcag
     4141 ggcgtcgctt tccgccggaa tgaaaatacg tcgctcgtac atccgcgcga gagtaaacaa
     4201 tgcagctccg gcaagcacat tcagcaaaat tgcgttaagg atcaacatgc gaatgcgttc
     4261 cagcacctta tcaaccggca ccgaatacac gatgcttagc gatgagggtg gcagattttt
     4321 cttcagcacc agctcccgga acccttccgt atagccaaac caggagcgtt cctgcatcca
     4381 gcgaggatcg cccttaattt tactttctgg tccggtaagc gaaatcaggg tatgaccatt
     4441 ttcatcaaga atggtaaccc ccatcggcaa cgtacccggt aagaaaaagt tctccatccg
     4501 gatggtctgc tcgacaccca aaagcgcctg caaccggttc gccagataaa ctggcgtcaa
     4561 cgcgtaaaaa tacccgacgc cagggcgcgg accttcgctg atccagtaga ggttactgcc
     4621 gctatcatct tgtggtgcat ttcgatattt attgatgcgt tcatgcaaag ctttcaacgc
     4681 ggtatcgcgt tccactggca tatcacgcag accgaaattg gccatgcaga ggttatcgct
     4741 gccgattaaa aatacccggt tgagatcgta agccgcagaa aaattatcgc gccagtagcg
     4801 cataaaccac gccaatgact ccagagaacc tcgccaggtg ttactcattg cggaacaatc
     4861 ggagtcggca aacagcggtt caaacgcagg cacatccgcc tgcgtttctc gtccacgcgg
     4921 ggaaagcaca ccgttttctg ccgataagcg attttcggcg atgtacttca gctctttcat
     4981 cacatcagag gtgcgttgaa taaagcgctg agcctgatcg gaactcagat taaattcctg
     5041 acgaatttcc gattctcgct gatgtaacgc attaacgatg taaaaaacgg atgaaaaagc
     5101 aatcaacagc cagagcacta acgccaatgc tctgaacatg tagcgcgagg ctttcagggt
     5161 tgtacgaaaa gaagcaaggt atttcaaagg ggcgaagctc cgcctcaggt gaccgatgga
     5221 gtgtggttaa ggtagcggta aaagcgtgtt accgcaatgt tctgtcacca ttgcaataat
     5281 gcgtatcgct tagaaaccat tgccttaacc aggaaagccg gagtagatgg acagttctca
     5341 gtacgaaaaa taagaaatga ccaatctgcc gacgcttgtt aatggttata tcctggcgtc
     5401 ggcagatcgc ccagcagtct tatctgaaaa aagagagagc caggttgtgt gaagggatgg
     5461 atcaccagtc aaaacttttc accagcgtca gctcgccagc attacgcatc ggtacaataa
     5521 atgtttcctg tttctcattg accgatcctt catcggtgat cagcgtcagt tgggcggtcg
     5581 tcaattccgt ttcgctgcgc ccaccatagt agttgatata cacctgatag cgcccgtgaa
     5641 ctggtgctgg catggcgaaa atctcgggtc cgtaccccgt cgtgacatcc atatccagtg
     5701 caccactgtt tttcagcacg gtgttaccgt accaggcgtg ttcgccatcg ggcgtgacaa
     5761 catggagatc gagatcggta ttgtccgtat cccacgagag aaccagccgt aaacgtgcac
     5821 gaatcgttcc tgtacccggc gttgagtaaa actgcatttt ttgtcggctt tgcccatccg
     5881 ggctgatgac ctgcacgctg ttgctgcctt cagtgaaaat ataagggcgt gcaaatgaac
     5941 catcggattc aactctctgc ggcatactgg caccgttgac caccagccgg ccctgctgaa
     6001 ctttacccgc cgcagcataa ttctttattt tgccgcggat ctgcgctgag atattttgat
     6061 catctgccat gttgacggac gacgccgggt aattgatcga ttgtgaaaaa ctggcatctt
     6121 cgccctctgc cggatgccag ccagagagcg gtgcgtcaat ctcgacttcc tgcacacctt
     6181 cactgtgagc gacaggcgaa agtgccacca gtaacaacgg aagaaaaatc tttcgcatag
     6241 cattagtcca ttaataattg tcgggtcagc gtttcgatat agttctcatc aagcccggtg
     6301 ggataaccat caaaggccag gtgcagatac tcatgggtta aatccagccg atcctgaagc
     6361 gtaaagaagt tacgaataaa cagccgtttc tgctgccgat cggtataggg gaaacctgag
     6421 actaaacgac agaccgcaaa cacgtctggt tcgttgtacc ccgtctcgcc ttgtaatata
     6481 cgccgccact gcggcatttt tttcgccagc caggctttcg ctttgggtaa taactggcag
     6541 gttgagcgtg gagctcccca gcggctaagg ctgttgtcgg gataagcaaa cgcgagtatc
     6601 tggtcgtaac gctccccctg ccccgcctgg gccgtggctt gccgccagga aagtgtccct
     6661 tcggtagcgc gactgccatg ataatgaacc ggatcgcctg catagatgag atcctgcgtc
     6721 caggcggtca tcgttcgcgc ccccgtggtc gctggcgaag cagaaacacg ctgcgtggcg
     6781 ctgctgtcgg ggatggtcag gcaatcgcct tcccgattgg cgttttgctg taaaaaagtg
     6841 cgaatcgcca cggtcatggc tttcgctgcc tcagggggcg tacttttcgc ctcgcgatcc
     6901 agaactctgg caacgtactc ttcacgatcg agatgcgact gcaatttcag tttgcccttt
     6961 tcgctcagta aggtcgtctc accgtggctg acaaacgtaa tgtggttacc attggtaaac
     7021 gtcacacgat agcgcccatt aagtacgccg ggttttactg ccgtcgtact cttttccgct
     7081 gtgattttct tcagcggata gcgggcaaac agctccacct ctacacattg cccactggca
     7141 acctgcgtcg gcactggcaa aacccgatta agcaccgtcg cataacgggt tagtaccgtt
     7201 ttgctggtgc cactgccagt agcccacagc ggtgtaccat cggttaacca gccggcaaaa
     7261 ccaccctgac ggatttcctg tttatcatcg gcaaaccagc tccaggtttt cacccgtacc
     7321 cgactaccga gcatggatgc gactccgatt ttcgcctcgt ccagcaccac atccagtaag
     7381 acttcctgag ctttgttttg cgcaggtaac gtcgctaatg aatcgagcag cgatttaacc
     7441 gttaccgagg tttctggctt cagcgttgta agagacgcta accacgcagg tgcctgacgc
     7501 tgttgccagt attgccccca cacatctgcg ccaatatgca accgctgcgg cgcaaaatag
     7561 agcccacagg aacgtaccag cgcggtatcg cgcgttatgc tctccccagc ctgacaacaa
     7621 taaacctctt ccggggagtt tccgcgacac tggtagggct gctccggttg atgggtatcc
     7681 tccagccagg cgtaaacata tagtttccac aaactcccta acggcgtggt gagcgagtca
     7741 ggtaatgctg agactttggt cacgcctgat gacgacagct gatacagttg atcgtgctgc
     7801 gcaccgcgca gcaccagttg taacggcgtc tcctccgcaa gcgttggcag agtaaccaac
     7861 gccaatagcc agacgattct gcgccagttc acttatttca cctgcatccc ggtccattca
     7921 ctccctgccg caacactttg ctgcgcaggt gcataggaac gcacataacg agcaggaggc
     7981 agaacgaatt gccctttttg cgagaagcgc agcaaatggc ggaaagtgac cgttcccgtc
     8041 agttctttca ccggcaccat ataggccagt tcgcccattt cattacgcgc tttttccagc
     8101 aattgcccct gctgtttcgc ggcgttgggt ttattgactg aaatgcccca tgttgtgcgc
     8161 tcaacgtcgg ctcccggcgg gagcggtact tccacctgac catagcgcag aactgcatcc
     8221 tgctcgctgg taagcgtaat ttcatcgaga tacagcgcat cgctgtcaat ctcattgctg
     8281 gtcaccggct gcagagtaaa gctcatctct tcttcaccgg ggataagccg atacaactgg
     8341 cgttcaacgg tcaccggaat gttactttgt tgagccgttt ttgccggttc acgccagcgg
     8401 acctgcacat tttgcggcga taattcgtca ccaaaagaga gaatgtccgg cacaccctga
     8461 ccaacccaac gccagtattc gccccctcca gttaacttat gtttagccca tgcgcccgca
     8521 ggcgcaggca aaacaactgg aggcattgtc gccatatatt tcgccagcca gttcatggcc
     8581 agcgcgcgct caatagtgga ttgctcagcg gtcaaaccac ttaaaatagc ggccgtctgg
     8641 gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg attgctgttt
     8701 gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt caactgcgct
     8761 tttttcagca acgtcatggt taagacgttt gccaccgcat ctcccagtgg agattccggt
     8821 gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa atcttcatct
     8881 ttagttccgc gccgggcgat agcttcatcc aaccctttca acaacgtttt gcacggcaga
     8941 ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat gttatccgcc
     9001 tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt tacgccgagc
     9061 gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc gtcaccattg
     9121 ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat cagccgcaga
     9181 cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg atcggcaagc
     9241 gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac gccaccccat
     9301 ggtccatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt ttcgctactt
     9361 tgtagccgga tattgctagc ctgctcgggc aacatcagcg cgttctcgcc accgccgagc
     9421 atgacatttt tctgctgttc aacgggccag ctgttatcca caaaagacag ttttgtgcta
     9481 atgctgtcct gcacttgccc attttgttgc agttctgcgc ttaacaagcc agattgctga
     9541 atgttctgcg ccagcgaaat ataattcgcc cctttgtgca gcgtcagcgt ctggcgcatc
     9601 tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg actgaagata
     9661 aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact ccacttcatg
     9721 tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc gtcgccgttc
     9781 atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa cgtgaaatac
     9841 gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc cacctcttca
     9901 cgccgtggac gttcaagcat ttttactcgc cgctcgctgc ggttagttgc gccaggcgca
     9961 accggctcgc tggagagcgc ctggtcgtag ctgataaacg acagactgga gctggtacgc
    10021 acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat ttctggttgc
    10081 agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac aggtttacct
    10141 ttcagcgacg aggttaattc gacattgacc agttcaccag gctggtaatg ggttttgtcc
    10201 gttttcaccc ggatatcaag ctgaggaacg gcaactttga tcccggcgtt ctgaaaactg
    10261 tactgaccat tacgggtata cagcaccgaa aaagtgatgt taggcgcaaa ggaattgctc
    10321 actggaaccc gggcttcata ctgggtatcg tttaaacgtt gtaacgtcag ccagtttgcc
    10381 ggatgcgaaa gcagcgactg ctgttccacg cgatcgcgtt ccagcgtcaa taatgcttca
    10441 tcaattggct ccggaaaggt aatcagcatc ttcgcagttt cgcctggctg gtacagcgtt
    10501 ttatccgcca cgatatctac cgtaccagta tgcgccgtgc tgcccttacc gctgacggca
    10561 tgacttaacc cagcgagaat taagccgtct ttatcgcgta atgtcagatt gtagttgcca
    10621 ggtttagcga aattgacggt aaaggatttg ccgcctgacg gtagctctcc gctatggctc
    10681 gtgcggtctt cgagacgcaa ccattcatac gtaacaggaa cctgttttga agattccagc
    10741 gcggcataac ggaacacaac cgactcgccg ctattactgt attgtgcggc agtacttaat
    10801 gagtaatgcg ccagaccgcg ttcaatgagg atctctttgg tggtggtgac acgatacgcc
    10861 gcgccgtcac tggcggagac ggttaacaaa taacggctcg gtttatcggc ggcggggaga
    10921 tttaacgcca catgaccgct ggcgtcggac accgtttcgc tgccttccag cgacacgggg
    10981 aaacgtccgg cataacgcaa atcgttaccg accattgata attgctgagc gcgcaaactt
    11041 aactgcacgc gggcattttt taccggctcg ccatccgggt agagcagttg cagtttgccg
    11101 ctgaccgctt cgccagtttt gaactctttt ttgtcgagag ctaaaccaat ctcgaaatgt
    11161 ggcttgatgt agtttgccac gcgaaaactg ctgctataga cctgattgcg gtaagcaaga
    11221 cgtaactcat aacctccggc aacggcattt tctggcaggc ggaaacttcc ctgcccgcca
    11281 ttgcgcgcat ccagcgtgac atcgacggtt tgcaacagac tgccgttagc gtccagcacc
    11341 gaaagcttcg ccggggcgct gacgatgggg gatgaatgca acggatcgtg gaactcgcgg
    11401 cccatcactt taacatcgac acgatcgcct gcgcgatata gcgggcgatc ggtaaaaata
    11461 tacaagcggg tgttgtagat ttcgctttcg tagaagaagt tctcggagac aaaaacaccg
    11521 ccttcagcat ccttacccag aatgtatgaa cgttctggcg atatatgttg taactgcaag
    11581 gtaccgctgt catcggtcac accgcgggtc atcacgccaa gaccgtcagt ccacaagatc
    11641 tctgagccgg gcttcgcttc accctgtttt ttacccgcgg tccacaccag aagctcgttg
    11701 cctgacactt tgctaagcgc cacggtatcg gaaacaaaca ccaccgtcgt cgcccgatac
    11761 ccaccaacca tcgcctcgac gaggtacagt cccggctctt gttggccgag aggaatataa
    11821 atgttacccg gctgcggcga gatgaaattg ctggatgcgc cttccagttt taccccttgc
    11881 tgcggctcga ccggtttagc ctgccatagt ggataacgga actgttccac caagggatat
    11941 tttttcagcg gggaaaactg gttgttctgt acataacggg aaggtttaat aatggcattg
    12001 ccgagatgta attcgggtaa tgcctgagtc acattctgcc gtgactgaga agagaaagta
    12061 cgctgcatta cgcggcgaga tttgccgtac cagttatccc acagccaggt cagcgtattg
    12121 ttcagcccgt cgcccagata ttgcggttgc accacaatgc gatgcaggtt tttctgctgg
    12181 cgcaaaaatg ccatcgggtc aggaatacga tacaggcgaa cgtccacgcc gccgtactct
    12241 tccatctgat agcgccgata atcacgcccc ggcgcttcca gtcgcacttt cgcctcttca
    12301 ctgctgctaa aactgctgtc agcaagcaaa aagaatgttc ccccggcggg cggcgcatag
    12361 ttactggaag gaagcgaatc atcagcatta gcaagccctg ttcccacaag agacagggac
    12421 aagcacgctg ctatcgcgcc agaaaattta aacgataaat gccaatgaaa ttgggattgg
    12481 aatcgttggg tatccatcgg gtgtccttcc atgtcataag ttgttgcaga ctgactgcgc
    12541 gcattccgtt gtcagttttc gtggcgcttc cggtgtggta gatgacgtaa cgccccatcc
    12601 agaccattaa gtgctgggca tcgccctgat cgaaaaaaat catatcgcca ggcagcgcct
    12661 ggtttatgtc ctggccaata aactggctgt tgtactgaat caaattaatc gcggtcacgt
    12721 aggggccggt tttcccgttc ccctgattcc agttttgcgc cagttgacgc tgtccaggtg
    12781 ttagcgtcat ctctggcggc aaatactggc tagagaaacc gttacttttt agccatttac
    12841 tatcgtgaac tttcagcgtc tcgttcgccg caaatcgcac caggcccgca caatcctgct
    12901 gataccagcg tggacttggc ccctggcgga gctgttcttg tgcaatgcgt acaaaccagg
    12961 cgcgaaacag cccggattgt tcgacattca gcatttcgct atgggcaaca acacaacaca
    13021 gccagcaaat cagcgccagc agcccgtgcc tcatagcggc tgccaggtta tgggtagcca
    13081 ctgccaggcg gcaccgggtt ccatctgggc cagcttcatg acataacgcg gttgttgaga
    13141 taaagcgtcc agcttcggca ttaataaagt ttgtgcggcg ttataaaaaa ccggttcgag
    13201 attcttcggc agactggtca acgtttcgtt acgcagcagt ttcgccacgc cttgtggatt
    13261 gatatagagc ggaacgatgc catcagtggg tattacatcc accattgccg ggcggttttt
    13321 attcagtgtt tgcagggcgt tattaaccag cgtttcatcg agggagaaaa gcagcgtttt
    13381 gttttgcatc gccagcgaca cgcggaaaaa ataatccgac attaattgat cgggttgcgc
    13441 cgcctgcgct ttcggatact gaccgtatcg ggaactcact tcgcgacgcc agatttgcgc
    13501 ttcgccctgc tgagtctggc ttatcggcaa aacaccttct ggcgctttgc tttcgtgcgc
    13561 accaatattt tgcgtaaaca gtttccctgg caattgcgcc tgttcggcag tgccatcaaa
    13621 ctgaccgaca aacagcgggg tttgcaattt tgagtctgca taccagcaca gccccgcggc
    13681 accgtctaac gccccattca acttgtcgtt ttcctgactg atgtgcgaaa gcatctcttc
    13741 ggcaataccg tgtgaatacg gcaccaccac acagaagctg gccccggcag gcatactgtt
    13801 ccagaccggc gtaaaatcga aactggtatc tacgctggcg gattcatcat ttaacgccac
    13861 aaaactgtgc cagccgtcgt tacccatttc gaagcgtacg ccagcaaaag aaggcattaa
    13921 ccgctggtag ccaaaaccca gcaacctggc gctgaccacg atgcgctggc gtactggcgt
    13981 tttttcagca gcacgctctt ccaggccaaa gcttgcttgc cagcgttttt tgccgctcaa
    14041 caaatcacct gcgatcgccg tggcttcggt atcctgctga tcatctttaa acaacatatc
    14101 cgtgctggaa aacaccagca tcttgtcctg ataagtcgcg aacatcagag cgttattgcc
    14161 gttatagcgc aactgataaa caggaacagt ttcactattt atcttgatgc tactgatttc
    14221 cgttttgctt aactggctgt cgctggtagc ggcaaacaac aatggctcca gcagtttgct
    14281 taaaccactg cgctggatca gcaccatata atgtgaaaga tggccttgtt tatcgtgcca
    14341 cagcgctgcc tgcgcgggct gatctaagag tgacgaaaag agcttatctt tcagcgtgag
    14401 atcgtgttca tagacaatac gacgaatgct gccttcaatg cccagacgat cggcatgatt
    14461 ctgataatag aaaacgaaat cttcgctcag aacatcgtgg agaaacggaa tggtgaggag
    14521 atctttggga agctggctca gagagtcgct gtcgagaaag aggtccggct cattgagatc
    14581 gatttgcaga ttgttgtgca ccaccagcgg cgacaacgtt ttttctggcc cactgccagc
    14641 atattgcaac gcccagacgc cagcggaaag cagtgctatt gcgccaaaac ctacaagacc
    14701 atagaaccgc cagcctttcg cctttttttc accactcatt gccacattcc ttgtgtatag
    14761 ccagccattt tttacgggca cagccaaact ttaccgtgcc ctaatacgac aaaagcccag
    14821 actttgcagc ctggactttt caattcaaac aagggagata gctccctttt ggcatgaaga
    14881 agtaaaatta ttcttcttct ggctcgtcgt caacgtccac ttccggagcg atttcatcgt
    14941 ccccttccgc ggcactgcca tcgatggtat ccagatcttc ctcgtcaacc ggttcagcaa
    15001 cacgttgcag acccactacg ttttcatctt ccgcagtacg gatgaggatc acgccctggg
    15061 tgttacggcc tacgatgctg atttccgaaa cgcgagtacg taccagcgta ccggcatcgg
    15121 tgatcatcat gatctggtcg cagtcatcta cctgtaccgc gccaaccact aaaccattac
    15181 gttcagtaac cttaatggag ataacccctt tcgtcgcacg cgacttggtt gggtattccg
    15241 ccactgcggt acgtttaccg taaccgtttt gcgttgcggt gaggattgcg ccatcgccac
    15301 gaggcacgat cagagagacg actttatcgc cttcacctaa gcgaataccg cgaacaccgg
    15361 tggtgttgca gcccatcgca cggacagaag actctttaaa gcgcaccact ttaccttcag
    15421 cggagaacag cattacttcg tcttcgccgc tggtcaggtc aacgccgatc agctcatcgc
    15481 cgtcaaccag tttgatcgcc actttaccgg cggtacgcag acggttgaac tcggtgagga
    15541 cagttttctt cacggtaccg ttagcggtcg ccatgaagac tttcacgcct tcttcaaact
    15601 cggtcactgg caggatcgca gtgatacgtt cgtcctgctc cagcggcagc aggttgacga
    15661 tcggacgacc gcgcgcgcca cgagtggctt ccggcaactg ataaactttc atcgaataga
    15721 cgcgaccacg gctggagaag cacagaatat ggtcgtgagt gttcgccacc agcagtcggt
    15781 cgataaagtc ttcttcttta atacgtgcgg cagatttacc tttcccgcca cgacgctgcg
    15841 cttcgtattc agaaagcggc tgatacttaa cgtagccctg gtgagagagc gtcactacca
    15901 catcttcctg agtgatcaga tcttccaggt tgatgtctgc gctgttggcg gtgatttcag
    15961 tacgacgttt gtcaccgaac tgttcacgaa ccagctccag ctcttcacgg atcacttcca
    16021 tcaaacgatc ggcgctacca agaatacgca acagttccgc gatctgatcc agcagctctt
    16081 tgtattcgtc gagcagtttt tcgtgctcaa ggccggtcag tttctgcaaa cgcagatcca
    16141 gaatcgcctg agcttgctgt tcggtcaggt agtacagacc atcacgcacg ccgaactctg
    16201 gctccagcca ttccggacgc gcagcatcgt cgccagcacg ttcgagcatc gcggcaacgt
    16261 tgcccagctg ccacggatta gcaaccagcg cagttttcgc ttctgcaggc gtcggcgcat
    16321 gacggatcag ttcgatgatc gggtcgatgt tcgccagcgc cacggctaat gcttcaagga
    16381 tatgagcacg atcgcgagct ttacgcagtt cgaaaatagt acgacgggtc accacttcac
    16441 ggcggtgacg aacaaacgcc gcgatgatgt ctttcaagtt catgatcttc ggctgaccat
    16501 ggtgcaatgc caccatgttg ataccgaaag aaacctgcaa ctgggtctgg gagtagaggt
    16561 tgttgagcac aacttcaccg accgcatcgc gtttcacttc aatcacgatg cgcataccgt
    16621 ctttgtcaga ctcgtcacgc agcgcgctga tgccttccac gcgtttttct tttaccagtt
    16681 ccgcaatctt ctcgatcagg cgcgctttgt ttacctgata cggaatttcg tggacgataa
    16741 tggtttcacg accggttttg gcgtcaactt ccacttctgc gcgagcgcgg atatacacct
    16801 tgccgcgacc ggtacggtaa gcttcttcaa taccgcgacg accgttaatg attgccgccg
    16861 tcgggaagtc cggccccggg atgtgttcca tcagcccttc aatgctgatg tcttcatcat
    16921 cgatatacgc cagacaaccg ttgatgactt ccgtcaggtt gtgcggcggg atgttggttg
    16981 ccatacctac ggcgataccg gaagaaccgt tcaccagcag gttaggaatt ttggttggca
    17041 tgacgtcggg aattttttcc gtgccgtcat agttatcaac gaaatcgacc gtctcttttt
    17101 cgagatcggc catcagttca tgggcaattt tcgccagacg gatttccgta taacgcattg
    17161 ccgccgcaga gtcgccgtcg atggaaccga agttaccctg accgtctacc agcatgtaac
    17221 gcagcgagaa tggctgcgcc atacggacga tcgtgttata aaccgccaag tcaccatggg
    17281 gatggtattt accgattacg tcaccaacga cacgggcaga ttttttatag gctttgttcc
    17341 agtcattgcc tagtacgttc atggcgtaaa gtacgcgacg gtgtaccggc ttcaggccat
    17401 ctcggacatc tggcagcgca cggccaacaa tgaccgacat cgcataatcc agataggagc
    17461 tcttcagctc ttcctcaatg ttgaccggtg taatttctct cgcaaggtcg ctcatctaac
    17521 cgctatccct ctactgtatc ccggattcaa aggtcgcaaa ttataacaca gccgcgcagt
    17581 ttgaggtaaa cctatacgct ttattcacat ccaatgcctg atatactcgt ttgtcttgcc
    17641 aattacggag tagaagtgcc aatgaatgcc gaaaaatcgc cggaaaacca taacgtagac
    17701 cacgaagaga tcgctaaatt tgaagccgtc gcctcccgct ggtgggatct tgaaggtgag
    17761 ttcaaaccgt tgcaccgcat taacccgctg cgtctgggct atattgccga gcgtgctggc
    17821 ggtttatttg gcaaaaaggt gctcgatgtc ggttgtggcg gcggcattct ggccgagagt
    17881 atggcgcgcg aaggcgcgac ggtgaccggt ctggatatgg gctttgagcc attgcaggtg
    17941 gcaaaactgc acgcgctgga aagcggcatt caggtggatt atgtgcagga aaccgtggaa
    18001 gagcacgcgg caaaacatgc cgggcagtat gatgtggtga cctgcatgga gatgctggag
    18061 cacgtccccg atccgcagtc agtggtcaga gcctgtgcgc aactggtgaa accaggcggc
    18121 gatgtctttt tctcgacgct taaccgcaac ggcaagtcat ggctgatggc ggtggttggc
    18181 gcggaatata ttttgcgcat ggtgcccaaa ggcacgcatg atgtgaagaa gtttattaaa
    18241 ccggcagaac tgcttggctg ggtagatcag acaagcctta aagagcgaca tataaccggg
    18301 ctacattaca acccgctcac taatactttt aaactcggcc ccggcgtgga tgtgaactat
    18361 atgctgcaca cgcagaataa gtgaggttgt tgtttggccg agccaatgcc tgatgcgacg
    18421 cttgccgcgt cttatcaggc ctacaaatgc tccccgtagg ccggataagg cgtttacgcc
    18481 gcatccggca accgtgccga ctagacagtg attaccattt caccgtcatc gacaaaaacc
    18541 ctgccatctg ggcaaaatca tcactccctt tctgccacgc cacgctgccg cgcagggaca
    18601 ctcgctgact gatattgccc gtgactccca cttttatttc accccgttgc ttcaccgcat
    18661 cgtcactgat agtgctgcca tcctcttcaa tttccgtcga atggggattg tgataataat
    18721 tcagatctaa tgttggtatg acatgaacag cggtacgcca ttcgctgtgt aaacccagcc
    18781 gcatctgaat atcatcaccc tgcgattgtg acacgcgcgc acggttagcg gcggtaaaat
    18841 catcctgctg cacgccctga taaatcacct gcgcctgcgg ttcaatcacc acaccacgcc
    18901 ccggtaacca ctgatacccg gcttccagcg aggcgataat ccccgacgag tggtaatgat
    18961 ctgtgccatc gtcttgttcg gaaacatcat tgttaaacca cgcgtattgc agccagctat
    19021 ccagccaggc cccctgcttc tgattaccgt gctgatacca gcttgaggtc agcccaacgg
    19081 cataaccgtg gttctggtta tcggcgcgag ttccggtcat attcgagcgg ctctcgccct
    19141 gattatcact gtagccacca accgccccaa gcatccactc gccatcatcg ccccagcgcc
    19201 cgctaaacag gtcaccgcta agctgcaccg tagaagtgtc ttcatgctga gccagttgcc
    19261 ctgccgccgt gtaatgataa cgtccgccga taacacgtaa attcagcgtc tgaccattgc
    19321 cacctgcatg atcgcgtcgc tccatcataa acgcctgatt tgccgcccgc agattattga
    19381 gataaccgcc aactttggca ttcagcaccg gctggtaagc ggggattgga tcgggtgttg
    19441 ggtcaggatc gggtgttggg tcagggtctg gatcaggtgg caatggtggc gttacttctt
    19501 gcgatcgcag ataccagtcg ttgttatctt ccaccagcgt gtagtcatat gctcccatat
    19561 tgacgtagcc gctgcctgcc agactgaact tcgcattgtt ttgaaactgc gtgggatcgg
    19621 ctgcgaaatc aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt
    19681 taaccaccac agttgtgttg ccagcagtat taccgttcaa caccaattga tcgcttaccg
    19741 agtcatcgcc gtttaattcg ctatcgagca gtagcgtacc gccgccggta taatcaccgt
    19801 ttaccgtcag cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg
    19861 tcagattgcc attaaccgtc acgtcggaaa gataggtcag gctgtcagtt tcaaggttgg
    19921 tattaagctc acctgtcaac gtcacgccat cggtggcatt caccagcgtc ccatcgccgt
    19981 taaacaatga ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac
    20041 catcgctgat atcgatagtg ccgctggaag tagcatcgat agactgaata tcctgatccg
    20101 cgccagtaac gaacgtcgcc ccatcaccga cccacagtga cgaagcataa ggaaagatat
    20161 ccgcaacatc gcctttcagg gtgccttctt cgacacgtac tgccgactgc gttgtaccgc
    20221 tggcggtcag ctccagcgta cccgcccccg ttttagtcaa tgtgctaccc tcatcctgat
    20281 gctgcccgct gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg
    20341 ccacttcacc gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag
    20401 tatcgacatt ggcagtaatc tccatcacgc tttcttgttg cttatcggta aagataacct
    20461 ggcggttata gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg
    20521 cgatttgtgt tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg
    20581 catcgaccac cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac
    20641 ctgcaagatc taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa
    20701 ggccatcatt gtatgatgtg ccgctctgcc aggtggagaa atcagagaga tcgagcacac
    20761 cgccgtttaa cacgatcgac tgcggatcgt cctggagagc ggcgagatct gccgcgtcgc
    20821 cttccagcga aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact
    20881 gcgcccccga cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga
    20941 tggtgccagc aaaactgcct tgattaacag taacattgcc accagcatcg atatttaaag
    21001 tgccattctg aaagcccgtc aatgagtgca caaaggtttg ttgggtggag ccaacgttta
    21061 gctctgcctg attctggtac ttatcaatac tccctatcgt cagaccgtag cagtcttgcg
    21121 gatcgtcctg gcaatgcgta tcgccgacat tcatcaggga gttgctgcgg cccagggtaa
    21181 cttcaccgtt ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca
    21241 gatcgccgga acctgttttg gtgatcaacc cggtaccagc aatagagtca acagctccgt
    21301 cattctctgt attgccaata accagcgttt ttccgtcggc aatatcaaag gtaacttcac
    21361 ttaagccgag atacataaag ccacccgccg cagtggaagg accatctcca tagcctgctg
    21421 cgctgttgtt ctcatccact aacacgccgc cgttctggct gtagctgtca tcaacagaaa
    21481 tatcaataag atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg
    21541 cagtgttatt tgtaaaggca gtgttattta ttatcgtata acctgaagga tgcttgctga
    21601 cgctattatt atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg
    21661 tatatgcctg gttgttatca aaaataacat cacttaaata gacgtcatta ttaatggtat
    21721 aaattgcgcc acctttgcca tcattagcga tattattgcg aaacatggcg ttagtgacac
    21781 gtaaatcgac ggcaccagta tcattagtac cagaagaata gattgcgcca ccatagccgc
    21841 ctgcgacgtt accagaaaaa ataacatcag taagattcag cgttgagttt tctttagcaa
    21901 atatggcccc gccgttatta tattctcctg taacggtgtt attggcaaac agagtcattc
    21961 cagtttcatt ttctggtaat agtgaaaatt ccgctccttg ttgcaaaaat acggctcctc
    22021 cgctggcgtt attggtcata tccgaaaaaa ccagccattg cccatcggcg acactccagt
    22081 cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg atatcatatc
    22141 cctggcataa atcaatggcc gccgcgaaac cgttagcaga gaaaagagat gcaatcattg
    22201 acgggagtag agataaatac tccttgcgta gaaagataat ccgcatattc atctaaccat
    22261 cattttatat aagacggcat atttaaacgc attatacaat atgggttatc tttcttttgt
    22321 aaattgtcca acccgatgca atggctatta tgttattatt tgatgaatag tatcagtgcg
    22381 tcataattca agttaataac cttaagggat accagttata tttaaactaa attaaagtaa
    22441 tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt taacaataac
    22501 cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgtttat atattgacca
    22561 caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc aatcattagc
    22621 aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt tcaagaaatc
    22681 gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg acaccttgtc
    22741 tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa gttatgcact
    22801 tgcaagaggg ccattttcac actatcttgc agtgaatccc aaacataccc cctatatata
    22861 gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc ggaaagaatt
    22921 ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg cgacggtagc
    22981 acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc agaaggactg
    23041 cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta tgacggtatc
    23101 aagacctctg acatccacga aaccattatc aaggcggccg cagacctgat ctcccgtgat
    23161 gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg taaaaaagcc
    23221 tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt cgagatgggc
    23281 aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca gatggacacg
    23341 tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca gctggaaggc
    23401 aaatatctgg tacagaaccg cgtgaccggc gaaatctacg aaagcgccca gttcctttat
    23461 attctggttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct gcaatatgtg
    23521 aagcgttttt acgacgcggt ttccacattt aaaatttcgc tgccgacgcc aatcatgtcc
    23581 ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg cggtgacagt
    23641 ctggattcca tcaacgctac ctccagcgcg attgtgaaat acgtttccca gcgtgccggg
    23701 atcggcatca acgccgggcg tattcgtgcg ctgggtagcc cgattcgcgg tggtgaagcg
    23761 ttccataccg gctgtattcc gttctacaaa catttccaga cagcggtgaa atcctgctct
    23821 cagggcggcg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca tctggaagtg
    23881 gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag gcaaccgcgt gcgccatatg
    23941 gactacgggg tacagatcaa caaactgatg tatacccgcc tgctgaaagg tgaagatatc
    24001 accctgttca gcccgtccga cgtaccgggg ctgtacgacg cattcttcgc cgatcaggaa
    24061 gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa gcagcgtgtg
    24121 aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg tcgtatctat
    24181 attcagaacg ttgaccactg caatacccat agcccgtttg atccggccat cgcgccagtg
    24241 cgtcagtcta acctgtgcct ggagatagcc ctgccgacca aaccgctgaa cgacgtcaac
    24301 gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg cgcaattaat
    24361 agcctggatg aactggaaga gctggcaatt ctggcggttc gtgcacttga cgcgctgctg
    24421 gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg tcgtacgctg
    24481 ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg ctactccgac
    24541 ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta cctgctgaaa
    24601 gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga aaccacttac
    24661 gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat cgctaatgag
    24721 ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg cctgcgtaac
    24781 tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa cgccactaac
    24841 ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg tattttgcgc
    24901 caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg ggaaatgccg
    24961 ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga tcagtcgatc
    25021 tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc gatgcagcag
    25081 ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta ttatcagaac
    25141 acccgtgacg gcgctgaaga cgcgcaggac gatctggtgc cgtcaatcca ggacgatggc
    25201 tgcgaaagcg gcgcatgtaa gatctgataa tcccccagcc tttgcaccaa accgtcccct
    25261 ctccccttgc gggagagggt tagggtgagg ggtaataaaa ccacaggaca cactcatggc
    25321 atataccacc ttttcacaga cgaaaaatga tcagcttaaa gaaccgatgt tttttggtca
    25381 gccggtcaac gtggctcgct acgatcagca aaaatatgac atcttcgaaa agctgatcga
    25441 aaagcagctc tctttcttct ggcgtccgga agaagttgac gtctcccgcg accgtatcga
    25501 ttaccaggcg ctgccggagc acgaaaaaca catctttatc agcaacctga aatatcagac
    25561 gctgctggac tccattcagg gtcgtagtcc gaacgtggcg ctattgccgc ttatttctat
    25621 tccggaactg gaaacctggg tcgaaacctg ggcgttctca gaaacgattc attcccgttc
    25681 ctatactcat atcattcgta atatcgttaa cgatccgtct gttgtgtttg acgatatcgt
    25741 caccaacgag cagatccaga aacgtgcgga agggatctcc agctattacg atgaactgat
    25801 cgaaatgacc agctactggc accttctggg cgaaggcacc cacaccgtta acggtaaaac
    25861 cgtaaccgtt agcctgcgcg agctgaagaa aaaactgtat ctttgcctga tgagcgttaa
    25921 cgcgctggaa gcgatccgct tctacgtcag ctttgcttgt tcctttgcct ttgcagaacg
    25981 cgaattgatg gaaggcaacg ccaaaattat tcgcctgatt gcccgcgacg aagccctgca
    26041 cctgaccggc acccagcata tgctgaatct gctgcgcagc ggcgcggacg atcctgagat
    26101 ggcggaaatt gccgaagagt gtaagcagga gtgctatgac ctgtttgttc aggcagctca
    26161 acaggagaaa gactgggcgg attatctgtt ccgtgacggt tcgatgattg gtctgaataa
    26221 agatattctc tgccagtacg ttgaatacat caccaatatc cgtatgcagg cagtcggttt
    26281 ggatctgccg ttccagacgc gctccaaccc gatcccatgg atcaacacct ggctggtgtc
    26341 tgataacgtg caggttgctc cgcaggaagt ggaagtcagt tcttatctgg tcgggcagat
    26401 tgacgcggaa gtggacaccg acgatttgag taacttccaa ctctgatggc ccgcgttacc
    26461 ctgcgcatca ctggcacaca actgctgtgc caggatgaac acccttccct tctggcggcg
    26521 ctggaatccc acaatgtggc ggttgagtat cagtgtcgcg aaggttactg cggctcctgt
    26581 cgcacacgcc tggttgcagg tcaagttgac tggattgccg aaccgttagc ctttattcag
    26641 ccgggggaaa ttttgccctg ttgttgccgg gcaaaaggcg atattgaaat cgagatgtga
    26701 attggttgta gtgccagata caacgcttat gcgtcttatc tggcctacaa cgattacatg
    26761 gcgtagtaat acgctttcac ctgctcccag tccgctttgg ggattggctc cagatatttt
    26821 tccagctggc ggaagtcatg attaatcgct ttatcgcgac gcaagcggcg acgacttttc
    26881 tccaggtcaa gaaaaccagc ttctgcctta ccttctgttt tcacatagat atggcgaaca
    26941 taacaacagc catgctgacg attaatgcta tgcattttct taaacgccag cgccactgct
    27001 ttcaacatgg cttgccgtac ttcgtcagaa taaggcgata ctgcatgctg ggcataccag
    27061 tcagcaatgc tgatgaaccc cgccatatct tcagtcacca gcaacgctcg ccattcaccc
    27121 tcaattttca ccgcttcacc aaaaacgatc ttcggcacga tgacaccggc ccgttccagt
    27181 tctttaatta ccgcaacctc acggacaatc gttggtcggc cgaacggata acgtacagaa
    27241 tgaaacagat gatgcgtcat gcgctttaca tacagctttt tgccgttgcg ctcgacgcat
    27301 tgcaccccgc tcataccatt acggcgatag ttaggctctt caacccagtc gccctctgtt
    27361 gcccaccagt gattaaattc gtcgtacttt gctgaaactg ccatacccta tcgcctgtca
    27421 tttttattaa cgacaatgac tataggtggt tacctgagga aaatcttaat gaaacgtgtc
    27481 gtattaatga atttcttttg ccactactag cgtgacaccg cttttaccct tcatttgcag
    27541 cgcatagatt ccaccagcca ctaccactaa tagtgcgccc agtagataaa atgtgaattt
    27601 aatcagtatg atacagcgat gaagtttatt cgacagggat taggcatcgc cttacaacca
    27661 gagttaacgc tgaaaagcat tgcaggtgaa ttgtgttccg ttcctcacga accaaccttc
    27721 tatcgacaga tttcgttgtt ggctaaagaa aagccggtag aaggcagtcc actgttttta
    27781 ctacaaacgt gtacggaaca actcgtggtg aatggaaaaa tttgatgtga gacaggatgg
    27841 cgcatgaacg ccatcctgca gtagcattac tctttattaa ggaattttac tgccttatca
    27901 gggaaatcag taaacagccc atttacaccc gctttgttat acagagcatc atataactga
    27961 ttcacatcag tagtgtattc aggcagttta tctgaccgca cggtataagg atgcactacc
    28021 agtttattct gctgagcatc ttgcaccatg ccagtgagtt tgatattacc cggctgcgat
    28081 gtttcctcaa tcaacatatg gtaatccggc ccgataccgt cggcatattc cgccacctgt
    28141 ttcatagcac ccggcttaaa catccagtcg tagttgtaat taacccagct tccatccggc
    28201 tgtttctgct gcgtttcatt ccagtcggta taggcaatca gctgtaccag attgagatcc
    28261 atgcccattt tgggttccag ctcattctta atacgcttca gctcatcagc atcaaaacat
    28321 tgcaaataaa ctttgtcgtc cttaccggtg taaccatatt tcttcagcac ttccagcgtt
    28381 tttgccgcaa tatccttccc ttcctgatga tggaaccacg gcgctttgat ttcaggataa
    28441 ataccgatat ttttcccggt agagtgattt aacccctgaa caaattcaat ctcttcttca
    28501 aaggtgtgca cccggaagtc ggacttaccc attgggaaac gccccggata agtctgcact
    28561 tttttaccgt tttcaatatc gaaaccttcg gtaaatttca gcgacttaat ttcatccagc
    28621 gtgaaatcta tcgcgtagta acgaccgtct ttgcgcgccc gatccgggaa acgatcggca
    28681 acatcagtaa cacgatcgag ataatggtca tgcagaacaa ccagatggtc gtctttggtc
    28741 atcaccaaat cctgttccag ataatccgct ccctgcgcat acgccatcgc ttttgctggc
    28801 agcgtatgct ccggcaaata tccactggca ccgcgatggg cgatgactat tttttcgttg
    28861 ctgtccgccg ccattgcact gcttcccatg actatagtgc tcatcatgat cgccatgcta
    28921 aggtttttca gcgtcaattt catgccatta gcctccgttg cgtttttgca gtaattgttc
    28981 atggcgacgt ttttcgccaa tcatcacaac aatcaacaag ataaccgcca gaatgctgcc
    29041 gccaatcatt accataaagc cgccatccca gccgaagaag tccacggtgt agccaacaat
    29101 cgcgctcgcc gccaccgaac cgcccaggta accaaacagc ccggtaaagc ccgctgccgt
    29161 acctgccgct tttttcggtg ccagttccag cgcatgcaga ccgatcagca tcacaggacc
    29221 gtagatcagg aagccgataa caatcataca aatcatatcg acggttgggt tacctgccgg
    29281 gttcatccag taaacgatag tcgcgatggt caccagtgtc ataaagaaaa cgccggttgc
    29341 cccacggttg ccacggaaga ctttatccga catccagccg cacagcagag tgcccggaat
    29401 acctgcatat tcataaagga agtaggccca ggaggattta tccagcgcga aatgcttaac
    29461 ctctttcaga taagtcggtg accagtcgag gatgccgtaa cgcagcagat aaacgaacac
    29521 gttggcgatg gcgatatacc acagcagttt gttcggcagt acgtactgca tgaagatttg
    29581 cttcgccgtc agctcctgtt ccgctttttc gttatagtcg tccggataat ca
//
LOCUS       DAEVZA010000051        28842 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEVZA010000051 DAEVZA010000000
VERSION     DAEVZA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.051"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..836)
                     /locus_tag="LAF92_002304"
     CDS             complement(<1..836)
                     /locus_tag="LAF92_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI9791303.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TS"
     gene            910..1440
                     /gene="thpR"
                     /locus_tag="LAF92_002305"
     CDS             910..1440
                     /gene="thpR"
                     /locus_tag="LAF92_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBI9791304.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            1455..2159
                     /gene="sfsA"
                     /locus_tag="LAF92_002306"
     CDS             1455..2159
                     /gene="sfsA"
                     /locus_tag="LAF92_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBI9791305.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            2337..2792
                     /gene="dksA"
                     /locus_tag="LAF92_002307"
     CDS             2337..2792
                     /gene="dksA"
                     /locus_tag="LAF92_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBI9791306.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            2829..3755
                     /gene="gluQRS"
                     /locus_tag="LAF92_002308"
     CDS             2829..3755
                     /gene="gluQRS"
                     /locus_tag="LAF92_002308"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBI9791307.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            3794..5212
                     /gene="pcnB"
                     /locus_tag="LAF92_002309"
     CDS             3794..5212
                     /gene="pcnB"
                     /locus_tag="LAF92_002309"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBI9791308.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            5209..5688
                     /gene="folK"
                     /locus_tag="LAF92_002310"
     CDS             5209..5688
                     /gene="folK"
                     /locus_tag="LAF92_002310"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBI9791309.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            6058..6642
                     /locus_tag="LAF92_002311"
     CDS             6058..6642
                     /locus_tag="LAF92_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9791310.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            6747..7487
                     /locus_tag="LAF92_002312"
     CDS             6747..7487
                     /locus_tag="LAF92_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBI9791311.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            7522..10122
                     /gene="htrE"
                     /locus_tag="LAF92_002313"
     CDS             7522..10122
                     /gene="htrE"
                     /locus_tag="LAF92_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HBI9791312.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            10139..10708
                     /locus_tag="LAF92_002314"
     CDS             10139..10708
                     /locus_tag="LAF92_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9791313.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            10723..11325
                     /gene="yadL"
                     /locus_tag="LAF92_002315"
     CDS             10723..11325
                     /gene="yadL"
                     /locus_tag="LAF92_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBI9791314.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            11352..11948
                     /locus_tag="LAF92_002316"
     CDS             11352..11948
                     /locus_tag="LAF92_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9791315.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            12001..13275
                     /locus_tag="LAF92_002317"
     CDS             12001..13275
                     /locus_tag="LAF92_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HBI9791316.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQ
                     QIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAMLSGTSVTG
                     STVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVGTENGQLLGNTLTG
                     NDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITY
                     PLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            13388..14182
                     /gene="panB"
                     /locus_tag="LAF92_002318"
     CDS             13388..14182
                     /gene="panB"
                     /locus_tag="LAF92_002318"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBI9791317.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            14194..15045
                     /gene="panC"
                     /locus_tag="LAF92_002319"
     CDS             14194..15045
                     /gene="panC"
                     /locus_tag="LAF92_002319"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBI9791318.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(15119..16021)
                     /gene="rpnC"
                     /locus_tag="LAF92_002320"
     CDS             complement(15119..16021)
                     /gene="rpnC"
                     /locus_tag="LAF92_002320"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBI9791319.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            16295..16675
                     /gene="panD"
                     /locus_tag="LAF92_002321"
     CDS             16295..16675
                     /gene="panD"
                     /locus_tag="LAF92_002321"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBI9791320.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(16679..17908)
                     /locus_tag="LAF92_002322"
     CDS             complement(16679..17908)
                     /locus_tag="LAF92_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBI9791321.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(17972..18412)
                     /locus_tag="LAF92_002323"
     CDS             complement(17972..18412)
                     /locus_tag="LAF92_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBI9791322.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(18517..19287)
                     /locus_tag="LAF92_002324"
     CDS             complement(18517..19287)
                     /locus_tag="LAF92_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9791323.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(19284..20210)
                     /locus_tag="LAF92_002325"
     CDS             complement(19284..20210)
                     /locus_tag="LAF92_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI9791324.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            20319..20981
                     /gene="can"
                     /locus_tag="LAF92_002326"
     CDS             20319..20981
                     /gene="can"
                     /locus_tag="LAF92_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBI9791325.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(21022..21558)
                     /gene="hpt"
                     /locus_tag="LAF92_002327"
     CDS             complement(21022..21558)
                     /gene="hpt"
                     /locus_tag="LAF92_002327"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBI9791326.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            21764..24154
                     /gene="gcd"
                     /locus_tag="LAF92_002328"
     CDS             21764..24154
                     /gene="gcd"
                     /locus_tag="LAF92_002328"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBI9791327.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(24201..25751)
                     /gene="cueO"
                     /locus_tag="LAF92_002329"
     CDS             complement(24201..25751)
                     /gene="cueO"
                     /locus_tag="LAF92_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBI9791328.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            25917..26264
                     /locus_tag="LAF92_002330"
     CDS             25917..26264
                     /locus_tag="LAF92_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBI9791329.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            26370..27236
                     /gene="speE"
                     /locus_tag="LAF92_002331"
     CDS             26370..27236
                     /gene="speE"
                     /locus_tag="LAF92_002331"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBI9791330.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            27252..28046
                     /gene="speD"
                     /locus_tag="LAF92_002332"
     CDS             27252..28046
                     /gene="speD"
                     /locus_tag="LAF92_002332"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBI9791331.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(28084..28446)
                     /locus_tag="LAF92_002333"
     CDS             complement(28084..28446)
                     /locus_tag="LAF92_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBI9791332.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(28621..>28842)
                     /locus_tag="LAF92_002334"
     CDS             complement(28621..>28842)
                     /locus_tag="LAF92_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791333.1"
                     /translation="FPNRLGTGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKT
                     AVDTYRYLNFNQLSQYTEKADGVIFQTAV"
BASE COUNT         7523 a         7266 c         6873 g         7180 t
ORIGIN      
        1 aaactggttt cagcaatatt ggtcgccagc accactttgc gcatcccttg cggtgccggg
       61 aggatcgctt ttcgctgatc gttcagcgac aacgcgccat acagcgggca gagcaacaca
      121 tcactgccga tgcgcgaagc cagttgttcc tgcacacgct gaatttctcc gacgccaggt
      181 aaaaataaca gtaatgatcc gctttcctga cgcagcattt cggcggtggc taccgcaacg
      241 gcttcgtcaa aacgctgatg cgcgggcagc ggtaaataat ggcgttcaac cggaaacgag
      301 cgcccttctg agatgacgac gggcgcttct ggcagcattt gctgcaagcg gtcgttatcc
      361 agcgtagccg acataatcag cagtttaagg tcatcacgca gaccttgttg cacatcgagt
      421 aacagcgcca acgccaaatc cgcctgcaag ctgcgctcat gaaattcatc gaggatcacc
      481 aatccaacac cgctcagttc cgggtcacgc tggatcatgc gcgtcagcac gccttcggta
      541 accacttcca ggcgggtatt cggcccgacg cagttttgcg cacgcatccg gtagccaacg
      601 gtatcgcctg gcttttcgtt aagcagctcc gccagtcgtt gcgcgacgtt acgcgccgcc
      661 agacgacgcg gctccagcag gataattttc ccgttaatgc cgggatgcgc cagcagttgc
      721 agcggcagcc aggttgattt cccggccccg gtcggcgcac ttaataatac ctgcggcgca
      781 caatcgagag cggtaagtaa ttcagataag acggcagcaa cgggcaacga cgacacaaaa
      841 cgctccagag ggttaacatt cttcgcgcca cattgtagca tcgcggtaat tcataaccga
      901 gtgcctcaca tgtctgaacc gcaacgtctg ttctttgcta tcgacttacc tgcagaaatc
      961 cgcgaacaga ttatccattg gcgcgccaca cacttcccac ctgaggcggg acgtccggtc
     1021 gccgccgata atttgcatct gactctggca tttttaggcg aagtgagcgc cgagaaagag
     1081 aaggcgcttt ctcttttagc cggacggatt cgtcaacctg gtttcacact cacgcttgat
     1141 gacgccggac aatggctgcg ttcgcgtgtg gtgtggttag ggatgcgtca gcctccacgc
     1201 ggcttaatcc agctggcgaa tatgctccgt tcacaggctg cccgcagcgg ttgttttcaa
     1261 agcaatcgtc cgtttcatcc acatattacc ttattgcgcg acgccagcga ggcggtgaca
     1321 atcccgccgc caggttttaa ctggtcttat gcggtgacgg agttcaccct ttacgcctcc
     1381 tcgtttgccc gtggacgcac acgctacacg ccgctaaaac gctgggcgct aacgcaataa
     1441 caaggattgt cgcaatggaa ttttctcccc ctctacagcg cgcgacgcta attcagcgtt
     1501 acaaacgttt tttagccgat gtgatcacac ccgatggtcg cgaattaacg ctacactgcc
     1561 cgaatacggg tgcgatgacc ggttgtgcaa cgcctggcga taccgtctgg tattcgactt
     1621 cagacaacac caaacggaaa tacccacaca cctgggaact aactcaaagc cagagcggcg
     1681 catttatttg cgtcaatacg ctttgggcta acaggttgac gaaagaggct atccttaatg
     1741 aatcaatttc agaactgtca ggctatagct cgctgaaaag cgaagtaaaa tacggcgcag
     1801 aacgcagccg tattgacttt atgttgcagg cggattcgcg tccagactgc tatattgaag
     1861 tgaaatcggt tacgttagcg gagaacgaac agggatattt tcccgatgcg gtcactgaac
     1921 gaggtcagaa acaccttcgg gagttgatga gcgtagcggc tgaaggccag cgtgcggtta
     1981 tctttttcgc cgtgctgcat tcagccatta cacggttttc acccgcgcgc cacatcgatg
     2041 agaaatacgc gcaactattg tcagaagctc aacagagggg ggtagaaatt ctggcttaca
     2101 aagcggaaat ttctgctgaa ggcatggctc ttaaaaaatc actgccggtt acattgtagt
     2161 aaagtaagta actggttaat ttacattctg gtcgcgtgcg caaatacgct tttcctcaca
     2221 cagttgtcaa gtgttacgtt tagataattg ctatccggaa aagcatctgc tatttatagc
     2281 ggcctcattt ttcccccgaa catggggatc gatagtgcgt gttaaggaga agcaacatgc
     2341 aagaagggca aaaccgtaaa acatcgtccc tgagtattct cgccatcgct ggggtggaac
     2401 catatcagga gaagccgggc gaagagtata tgaatgaagc ccagctggcg cacttccgtc
     2461 gtattctgga agcatggcgt aatcaactca gggatgaagt cgatcgcacc gttacacata
     2521 tgcaggatga agcagccaac ttcccggacc cggtagaccg tgcagcccag gaagaagagt
     2581 tcagcctcga actgcgtaac cgcgatcgcg agcgtaagct gatcaaaaag atcgagaaga
     2641 cgctgaaaaa agtggaagac gaagatttcg gctactgcga atcctgcggt gttgaaattg
     2701 gtattcgccg tctggaagcg cgcccgacag ccgatctgtg catcgactgc aaaacgctgg
     2761 ctgaaattcg cgaaaaacag atggctggct aattacagcc gttccatcac gtttaccaca
     2821 ggcgggaaat gctcccgcct tattttttgt tcaaagagat gacagacaca cagtatattg
     2881 gccgcttcgc cccctctcct tccggcgagc ttcattttgg ctctctgatc gccgcgctcg
     2941 gcagctattt gcaggctcgc gcccggcaag gtcgctggct ggtacgcata gaagatatcg
     3001 acccgcctcg tgaagttccc ggtgccgcag aaactatcct gcgccagctg gaacattacg
     3061 gtctgcactg ggacggcgat gttctctggc aatcgcaacg tcaccacgcc tatcgtgaag
     3121 cactcgcctg gttacatgaa caaggactaa gttattactg cacctgtacg cgtgcgcgta
     3181 ttcaaagcat tggcggtatt tacgacggtc attgccgggt gttgcatcat ggaccagaca
     3241 atgccgcagt gcgtatccgc cagcagcatc cggtcacgca atttactgac cagctgcgcg
     3301 gcattattca cgccgacgaa aaactggcac gggaagattt tatcattcat cgccgtgatg
     3361 ggttgttcgc ctacaacctg gctgttgtgg ttgatgatca tttccagggc gttacagaaa
     3421 tagtgcgtgg ggctgatctg attgaaccaa cagtaaggca aatctcgctg taccagcttt
     3481 ttggctggaa agtgccagat tacattcatc tgccgctggc gcttaatcca caaggcgcta
     3541 aactttccaa gcagaatcat gcgcctgcgt tgccgaaagg cgatccacgc ccggtactaa
     3601 tcgcggcact tcaatttctg gggcagcagg cagaagcaca ctggcaggat ttcagcgtcg
     3661 agcaaatcct tcagtcagcc gtcaaaaact ggcggcttac cgccgtgccc gagtcggcaa
     3721 ttgtaaattc aacattctca aatgcgtcat gctgagctat gattagccgc tatttttttg
     3781 tcctgaatga tgtttgacac taccgaggtg tactattttt acccgagtcg ctaatttttg
     3841 ccgcaaggtg ctaagccgcg aggaaagcga ggctgaacag gcagtcgccc gtccacaggt
     3901 gacggtgatc ccgcgtgagc agcatgctat ttcccgcaaa gatatcagtg aaaatgccct
     3961 gaaggtaatg tacaggctca acaaagcggg atacgaagcc tggctggttg gcggcggcgt
     4021 gcgcgacctg ttacttggca aaaagccgaa agattttgac gtaaccacta acgccacgcc
     4081 tgagcaggtg cgcaaactgt tccgtaactg ccgcctggtg ggtcgccgtt tccgtctggc
     4141 tcatgtgatg tttggcccgg agattatcga agttgcaacc ttccgtggac accacgaagg
     4201 taacgtcagc gaccgcacga cctcccaacg cgggcaaaac ggcatgttgc tgcgcgacaa
     4261 cattttcggc tccatcgaag aagacgccca gcgccgcgat ttcactatca acagcctcta
     4321 ttacagcgta gcggatttta ccgtccgtga ttacgttggc ggtatgaagg atctgaagga
     4381 cggcgttatc cgtctgattg gtaacccgga aacgcgctac cgtgaagatc cggtacgtat
     4441 gctgcgcgcg gtacgttttg ccgccaaatt gggtatgcgc atcagcccgg aaaccgcaga
     4501 accgatccct cgcctcgcta ccctgctgaa cgatatccca ccggcacgcc tgtttgaaga
     4561 atcgcttaaa ctgctacaag cgggctacgg ttacgaaacc tataagctgt tgtgtgaata
     4621 tcatctgttc cagccgctgt tcccgaccat tacccgctac ttcacggaaa atggcgacag
     4681 cccgatggag cggatcattg aacaggtgct gaagaatacc gatacgcgta tccataacga
     4741 tatgcgcgtg aacccggcgt tcttgtttgc cgccatgttc tggtacccac tgctggagac
     4801 ggcacagaag atcgcccagg aaagcggcct gacctatcac gacgctttcg cgctggcgat
     4861 gaacgacgtg ctggacgaag cctgccgttc actggcaatc ccgaaacgtc tgacgacgtt
     4921 aacccgcgat atctggcagt tgcagttgcg tatgtcccgt cgtcagggta aacgcgcatg
     4981 gaaactgctg gagcatccta agttccgtgc ggcttatgac ctgttggcct tgcgagctga
     5041 agttgaacgt aatgctgaac tgcagcgtct ggtgaaatgg tggggtgagt tccaggtttc
     5101 cgcaccacca gatcaaaaag ggatgcttaa cgagctggat gaagaaccgt caccgcgtcg
     5161 tcgtactcgt cgtccacgca aacgcgcacc gcgtcgtgag ggtaccgcat gacagtggcg
     5221 tatattgcca taggcagcaa tctggcctct ccgctggagc aggtcaatgc tgccctgaaa
     5281 gcattaggcg atatccctga aagccacatt cttgccgttt cttcgtttta ccgcacccca
     5341 ccgctggggc cgcaagatca acccgattac ctcaacgccg ccgtggcgct tgaaacttcg
     5401 ctcgccccgg aagagttact caaccataca cagcgcatag aactgcaaca gggtcgtgtt
     5461 cgtaaagctg aacgctgggg accacgcacg ctggatctcg atatcatgct gtttggtaat
     5521 gaagtgataa ataccgaacg cctgaccgtt ccgcactacg atatgaagaa tcgtggattt
     5581 atgctgtggc cgctgtttga gattgcaccg gagctggcgt ttcctgatgg ggagacgttg
     5641 cgtgaagtct tacatacaag agcatttgac aaattaagca aatggtaatt tatttatatt
     5701 acgaatataa atattttctg aaaatcatac atcctctctt tccttaataa aaattttagt
     5761 ttaaacattt ttgcagaaac ataatataaa ttcccgcaga taaatatcaa tcccatcgcc
     5821 ataccctaac caattgcact tgtgaattta tttttgctta attttgaatt aagaaaattt
     5881 aatttgttca aaagccctta aagtgcacaa taggcgtcct aatgaacaat ggttgtaaat
     5941 tttggtaaca aatatcattg ctcattaata tctagcatct gcttataaca tttgtgtcat
     6001 gaatagtcat tcttcaaacc agtagtctga ctaatggaat tttattcaag gatttttatg
     6061 tctaaaaaat taggttttgc cctgtctggt cttatgctgg caatggttgc aggtactgct
     6121 tctgctgata tggatggtgg tcagttaaat atcagtggtc tggttgttga taacacctgt
     6181 gaaacccgcg ttgacggcgg taataaagat ggtctgatcc tgctgcaaac tgcaactgtt
     6241 ggtgaaatcg atgctggcgt gctgaatgac accgttggcg ctaaagccaa acctttcagc
     6301 atcaccgttg actgcagcaa agcaaatccg aatccaggta gtacagctaa aatgaccttc
     6361 ggttctgttt tctttggtaa tagcaaagga acgctgaaca acgacatgtc tattaacaac
     6421 ccgtctgacg gcgtgaatat cgctctgcac aatattgatg gttccactat caaacaggtt
     6481 caaattaaca accctggcga tgtgtatact aaagccctgg atgcaacgac aaaatctgct
     6541 gtttatgatt ttaaagcgtc ttacgttcgt gccgtcgcag accaaacagc aactgctggt
     6601 tatgtaaaaa ctaacactgc atacaccatt acttatcagt aagtattcac caattaaatt
     6661 gattgaatga atatacaggg aatagtgtta ataatttcta tttttatatt attccctgtt
     6721 ttaattaact ctgtcaggga tggtttatgt tttttaacac caaacataca acagctttat
     6781 gcttcgtaac ctgtatggct tttagttcat cgtctattgc ggacattgtc atttcgggta
     6841 ctcgcgtaat atataaaagc gatcaaaaaa gtgtcaacgt acgtctggaa aataaaggga
     6901 ataacccgtt gcttgtccag agttggttag atactggcga tgacaacgct gagcctggca
     6961 gtattacagt cccttttact gccacgccgc cagtatcgcg tattgatgcc aaacgtgggc
     7021 aaacaatcaa attaatgtac acagccagca cctcactgcc taaagacaga gagagcgtgt
     7081 tctggtttaa cgtactggaa gttccaccaa aaccagatgc agaaaaggtc gcgaatcaaa
     7141 gtctgctgca actggcattt cgcacacgta taaaactttt ctatcgcccg gatggattga
     7201 agggaaatcc ctctgaagcc ccgttagccc tgaagtggtt ctggtcaggt tcagaaggta
     7261 aggcgtcatt gcgagtgacc aatccaaccc cttactacgt ctcttttagc agtggtgatt
     7321 tagaagctag cggtaaacgc tatccgattg atgtgaaaat gattgcacca tttagtgatg
     7381 aggtcatgaa agtcactggc cttaatggca aagcaagttc tgcaaaagta catttttacg
     7441 ccattaatga ttttggtggc gccattgaag gtaatgccag tctgtaatca ggcaaggata
     7501 taattccgca ggaagcataa cgtgactata aaaccaacta aaaattatca taatcatctg
     7561 acccgtatcg ctacgttttg cgcgctgctg tattgcaata gtgctttctg tgctgaactt
     7621 gttgaatatg accatacctt cctgatgggg cagaatgcct caaatattga tctcagccgg
     7681 tatagtgaag gtaaccctgc tataccgggt atgtacgacg tcagtgttta tgtcaacgat
     7741 caacctatta ttaaccaaag cattacattt attgaaattg aaggaaaaaa aaacgcacaa
     7801 gcctgtatca cacttaagaa tttattgcag tttcatataa attctcccga tataaataac
     7861 gaaaaagccg ttctgcttgc cagggatgaa acactcggca attgccttaa tttgacggaa
     7921 attatccctc aggcttctgt tcgttatgac gttaacgagc aacgtctgga tatagacgtt
     7981 cctcaggcat gggtaatgaa aaattaccaa aactatgttg atccatcgtt atgggaaaat
     8041 ggcattaatg cggccatgtt gtcatacaac ctcaacggat atcatagtga aactcctggt
     8101 cgaagaaatg acagcattta tgctgctttt aacggtggga tgaatttagg tgcatggcga
     8161 ctgcgtgcct caggcaacta caactggatg accgattctg gtagtaatta tgattttaag
     8221 aatcggtata tacagcgtga tatcgcctcg ctgcgttctc aactcattct tggtgagtct
     8281 tatacaacgg gcgaaacctt tgattctgtc agtatccgcg gcattcgttt atacagcgac
     8341 agccgcatgt tgcctccgac attagcgagt tttgcgccta tcattcatgg cgttgctaat
     8401 accaacgcca aagtcactat tacgcaaggt ggctataaga tttatgaaac gactgtgccg
     8461 ccaggcgctt tcgtcattga tgatctgagt ccgtcaggtt acggcagcga tcttattgtt
     8521 actgtcgaag aatccgatgg ctcaaagcgg acattctcgc aacctttttc atcggtcgtt
     8581 caaatgttac gccctggcgt tggacgttgg gatattagcg gcggccaggt cttaaaagat
     8641 gatattcagg atgagcctaa tttatttcag gccagctact actatggtct gaataactat
     8701 ctaacgggtt atactggtat tcagattacc gataataact ataccgctgg tttgttaggt
     8761 cttggtctga atacttcagt cggtgcattt tctttcgatg tgactcactc caatgttcgt
     8821 ataccggatg ataaaacata tcagggacaa agttatcgtg tttcctggaa caagttattc
     8881 gaagaaacaa gtacttcact gaatatcgcg gcctatcgct attcgacaca gaattacctt
     8941 ggtcttaatg atgcactaac tctaattgat gaagtgaaac atcccgaaca agatcttgaa
     9001 ccgaaatcca tgcgtaatta ctcgcgcatg aaaaatcagg ttacggtcag tattaaccaa
     9061 ccgctaaaat ttgagaaaaa agattacggt tcattttatc tttccggaag ttggtccgat
     9121 tactgggctt ccggacaaaa tcgtagcaat tactctattg gctacagtaa cagtgcatcc
     9181 tggggcagct acagtgtcag tgcccagcgt tcatggaatg aagacggcga cactgacgat
     9241 agcgtttatc tcagtttcac cattcctatt gaaaaattac ttggcactga acaacggact
     9301 tcaggtttcc agagtattga tactcaaatg agcagtgact ttaagggtaa taaccaactc
     9361 aacgtcagta gcagtggcta tagtgataac gctcgcgtca gttatagcgt aaatactggc
     9421 tatacgatga ataaagccag caaagatttg agttatgttg ggggttatgc cagctatgaa
     9481 tcaccgtggg gaagcctggc aggttcagtt tctgcaaata gcgataacag tcgtcaagtg
     9541 tctctcagca ccgacggtgg ttttgtattg catagcggcg gcctgacctt cagtaatgac
     9601 agttttagcg actccgatac actggcggta gttcaggctc caggtgctca aggagcgcga
     9661 ataaattatg gcaacagtac tatcgatcga tggggttatg gtgtcaccag cgctctttct
     9721 ccttatcatg aaaaccgtat cgcgctggat atcaacgatc ttgagaacga tgttgaatta
     9781 aaaagtacca gtgcagtagc tgtaccgcgt cagggttcag tcgtctttgc tgattttgaa
     9841 accgtgcaag ggcaatcagc cattatgaac atcacacgaa gtgatggtaa aaatattcca
     9901 tttgctgcag atatttatga tgagcaaggc aatgtcattg gtaatgttgg acagggtgga
     9961 caagcatttg ttcgtggtat tgagcagcag ggaaatatca gcattaaatg gctcgaagaa
    10021 agtaaacccg taagttgtct tgcgcattat caacaaagtt cagaagcaga aaaaatagcg
    10081 caatctatta ttctgaatgg aattcggtgt cagattcagt aactacaagg gacgtcaaat
    10141 gataaaaata acgccacata aaatagcgat actgatggga atattattat caccatcagt
    10201 atttgcaacg gatattaatg tagagtttac agccactgtc aaagcgacaa cctgtaacat
    10261 cacacttact ggtaataacg tcacgaatga tggcaataat aactacacat tgagaatccc
    10321 taagatgggt ctggataaga tcgcgaataa aacgacagaa tctcaggctg attttaaact
    10381 ggttgctagt gggtgcagca gtggcatcag ttggattgat accactctga ccggaaatgc
    10441 atcatcaagc tcacctaagc ttattatacc gcagtctggt gattcatctt cgacgacaag
    10501 taatatcggt atgggtttca aaaaacggac tactgatgat gccactttcc ttaaacctaa
    10561 cagtgcggaa aagatacgct ggagcacaga cgagatgcag cccgataagg gtcttgaaat
    10621 gaccgttgcg ctacgtgaaa cagatgcagg gcaaggcgta ccggggaatt ttcgtgcact
    10681 ggcaacgttt aatttcatct atcaataaga aggataatta agatgacatt taaaaattta
    10741 cgttatggat tatccagcag tgtcgttttg gctgcctcat tgttcagcgt accctcttat
    10801 gcggcaacag acagcattgg gctgaccgtg attactaccg tagaaatagg tacttgtact
    10861 gctacattag taaatgactc tgatcaggac atttctgttg ttgactttgg cgatgtatat
    10921 atttctgaaa tcaatgccaa gaccaaagtt aaaacattca aactcaaatt caaagactgt
    10981 gcgggtatcc ccaataaaaa agcacaaata aaattaacca agcgagccac atgcgaaggt
    11041 actgctaatg acggtgcggg gttcgcaaat ggatccacag ccgcagataa agcaagtgcc
    11101 gtcgccgttg aagtctggag caccgaaact ccggcaacag gtagcgcaac acaatttagt
    11161 tgtgtgacac cagcatctca agaggtcact atctccaatg cagccaacgc ggttgtttat
    11221 tatccgatga gtgctcgact ggtcgtcgaa aaaaataaaa ccgtaagcaa tgtcactgcg
    11281 ggtaaatttt ctgcacctgc cacatttaca gtaacctata actaacagac tgacagatcc
    11341 aggaactata tatgcatccc actcaacgta agctgatgaa gagaataatt ctgtttctgt
    11401 cactacagtt tcccattacc tatccagcca tcgctgggca ggatattgac cttgttgcca
    11461 atgtaaaaaa cagcacttgc aaaagcggaa tcagtaacca gggtaatatt gatcttggcg
    11521 tcgttggggt gggatatttt tcagataatg ttactcctga aagctatcaa ccaggtggaa
    11581 aagagttcac tgtcacagta tcagactgtg cattacaggg aactggcgat gtgcttaatc
    11641 agttacatat tgattttaga gcccttagcg gtgtcatggc tgcaggttca aggcaaatat
    11701 ttgccaatga agtcgcaacc ggggctaaaa atgtcggtgt cgttctcttt tccattcagg
    11761 acccgaccaa tatattcaat gttattagtt cagcaggaaa ttctcgttcc gtgtatccag
    11821 taatgacttc tgccctgcat aattcttcat ggaaatttta tgccagaatg caaaaaatcg
    11881 atcccgcgct ggatgtcata tctggccagg taatgagtca tatattagtt gatgtgtact
    11941 acgaataata gatcaaaaca aataactgcg actgttacag cagtagatgg tggagtgata
    12001 atgaagaaat tcttcagaca ttttcttttt ttaattctgt gcttgagttg ttataccgcg
    12061 agtgctggca ctgatgataa cgtaggatat attgttggga atagttacgg cgttggtccg
    12121 tcagatcaaa aatggagaga aacggggccg aatggcgatg ctactgtcat attcagatat
    12181 gcaacaagta caaataattt agttttctac aaaccgacgc agcttggacc tacaggtgta
    12241 aaattacagt ggagtcagtt agataccgct tctggtggtg gttttcttta ttgcaacaga
    12301 agtgatagca caagtggtag cgcaatgcgt attgaaaatg caatggttga ctcaggtaaa
    12361 atgtatggct cccataaatt atttaataca tcagttcctg gtttgtatta cacattatta
    12421 atttcaaaca tgtggtcagc ttacggtacc gtaactaacg ttagttcacc tgggatatac
    12481 attggtgact ctgcagaaca atatttttcg tggtataatc caagcgaaga cgtgttatat
    12541 tggagttgca ataatgcgaa tagcacccgt aaatactggg ctgtaggtgg tatttatcag
    12601 acccttacaa ttgaattcta tacagataca aactttgatc caacagtaac tcaacagatc
    12661 aaattatcca gctcatcaaa ttatctgtat tcatttaaag cctatggcgc aggccaaggt
    12721 ataaatgagc atagttattt tatcaaaatc gatttcgatc tgctcaatgt caagttgact
    12781 aatcccactt gttttaccgc tatgcttagt ggaacttctg tgactggttc tacggtaaaa
    12841 atgggtgaat atagtgcaga acaaatcaga aacggtgcca caccggttcc ttttgatatt
    12901 tcacttcaaa actgcgttcg tgtgactaat attgagacaa aattagtttc aacaaaggtt
    12961 ggtactgaaa acgggcaact ccttggtaat actctcacag gtaatgacgc agcaaaagga
    13021 gtcggtgtac tcatagaagg tttagcaact agtaaaaatc ctctaatgac attgaaacct
    13081 aatgattcaa attctgttta taaagattac gacccgagag gcaaagacga tacaacagga
    13141 ggagtttacc cggatcaaga tacaggtata acataccctc tccatttcca ggccacgcta
    13201 caacaggatg gaactatacc aatagaagct ggtgagttta aagccaccag tactttccag
    13261 gtaacctacc cttaataagt ccaccgcacc gccatcatct ggcggtgcgg taattgataa
    13321 atcattgccc ccaccaatgt gactgataaa atgccaggca acacgacatc atttatcagg
    13381 acacgttatg aaaccgacca ccattgcttc acttcagaaa tgtaagcagg ataaaaagcg
    13441 tttcgcgacc atcaccgctt acgactacag cttcgcgaaa ctatttgctg atgaaggact
    13501 taacgtcatg ctggtaggcg attcgctggg catgacggtt cagggccacg actccaccct
    13561 tcccgttacc gtcgaggata tcgcctatca taccgccgcc gtacgtcgcg gcgcgccaaa
    13621 ctgcctgctg ttggctgacc tgccgtttat ggcgtatgcc acgccggaac aagcctttga
    13681 aaacgccgca acggttatgc gtgccggtgc caatatggtc aaaattgagg gcggtgagtg
    13741 gctggtcgaa accgtaaaaa tgctgaccga acgtgccgtt cctgtatgtg gtcacttagg
    13801 tttaacccca cagtcagtga atattttcgg tggctacaaa gttcaggggc gcggcgatga
    13861 agcgggcgat caactgctca gcgatgcatt agccttagaa gccgctgggg cacagctgct
    13921 ggtgctggaa tgcgtgccgg ttgaactggc aaaacgtatt accgaagcac tggcgatccc
    13981 ggttattggc attggcgcag gcaacgtcac tgacgggcag atcctcgtga tgcacgacgc
    14041 cttcggcatt accggcggtc acattcctaa attcgctaaa aatttcctcg ccgaaacggg
    14101 cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg tttatccggg
    14161 cgaagaacac agtttccatt aaggagtcac gttgtgttaa ttatcgaaac cctgccgctg
    14221 ctgcgtcagc aaattcgccg cctgcgtatg gaaggcaagc gcgtggcgct ggtgcctact
    14281 atgggtaatc tgcacgatgg ccatatgaag ctggtcgacg aagccaaagc ccgcgccgat
    14341 gtggtcgtcg tcagtatttt cgttaacccg atgcagttcg accgcccgga agatctggca
    14401 cgttatccac ggaccttgca ggaggactgc gagaagctaa acaaacgtaa agtggattta
    14461 gttttcgccc cttcggtaaa agagatctac ccgaacggta ctgaaaccca tacttacgtt
    14521 gacgttcccg ggctttcgac catgctggaa ggcgcgagcc gtccggggca ttttcgcggc
    14581 gtttcgacta tcgtcagcaa gctgtttaac ctggtccagc cggacatcgc ctgcttcggt
    14641 gaaaaggact ttcagcaact ggcgctgatc cgcaaaatgg ttgccgatat gggcttcgat
    14701 attgagattg tcggtgtgcc aattatgcgc gccaaagatg gccttgcgct cagttcccgt
    14761 aatggttatc tgacggcgga acaacgcaaa attgcgcccg gtctgtacaa agttttaagt
    14821 tcaattgccg acaaattgca ggctggggaa cgggatctcg atgaaattat taccattgcg
    14881 gggcaagaac tgaatgaaaa aggcttccgc gccgatgata ttcagattcg cgatgccgac
    14941 acattgctgg aagtttctga aaccagcaaa cgggcagtaa ttctggtagc cgcctggctt
    15001 ggcgatgctc gcctgatcga caacaaaatg gtcgagctgg cgtaatactt aactggcgct
    15061 acggctgatg gcgccagtta ttaatttacc ccacgtcatc tgtcataaca caggtaactt
    15121 aaataaggtt aattacctta tcaatctcag caagaggtaa atttgccatc tctgcaacgt
    15181 cttcccgaga cattccttta cttagaagac gcagggcgaa ttcttgcctt tcctcctgtc
    15241 ttccctgctg aatccccttc tcaatccctt tctcttcaaa ccactgcgcc agcgtcatca
    15301 tagactcccc tcccgtttcc ctgtctctca gcacaccata aaacaaatcc gcttgttcag
    15361 tatgaccgcg ttgcagcata tagttttgca tggcaactaa ctgacttccg ctagtgtacc
    15421 cttcgtcaat cagcgtgacc agttgctcca gcaataacat taagtcgcgc tggcgaatat
    15481 gtttttgcag tagttcgaga atcgcaatcc gccgatgttg catgatttcg tcatccggtg
    15541 tgatggtgat atccaccagc gggaaaggac tgttatagac gcgtcgtgcc agctccggcg
    15601 agtaaaatat atcaaaccag cacattgaga gcggataagg tgtggcctcg ccctgataaa
    15661 acagtatcgg caccaccagc ggcagcttat cgtgatccgc ctccagatgc cggtgcatgg
    15721 cagcaataga ataacgcatc atgcgaaagg ccattttctt atccggcttg ctttggtgtt
    15781 caatcacaac atgcagataa ccgggattgc cctgcatttg cacggaatag agcacgtccg
    15841 tgctgtgtcc tttcaggctc tcttcaatga aactccccga ctctaaatga agcgtgttga
    15901 ggtcacaaag ttctcgtaat tccactggca aatgtatctc cagaaagtcg cgagccgtct
    15961 ccgcgtgcat taaaaattgt ttaaacactg catcatgcgg tgtggtactc ggtgcatcca
    16021 tcttccctgc cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg cgtgccatcg
    16081 tcagccacct ctttagtgtt taccgagcag cgttcagaga aatttcaatt ggttacagcc
    16141 gttacatggc acgcttcggc acggtacgca aactgctgat gtaaatgccg tcagatgagt
    16201 agacactaaa caaaaatcgg gcaatactgc gtgagaattt cctcaaagca ggcatcgcct
    16261 gcttcgttaa cgacagggta gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa
    16321 actccaccgc gtgaaagtga ctcatgcgga cctgcactat gaaggttctt gcgccattga
    16381 ccaggatttc cttgacgcag ccggtattct cgaaaacgaa gccattgata tctggaatgt
    16441 caccaacggc aagcgtttct ccacttatgc catcgcggca gaacgcggtt cgagaattat
    16501 ttctgttaac ggtgcggcgg cccactgcgc cagtgtcggc gatattgtca tcatcgccag
    16561 cttcgttacc atgccagatg aagaagctcg cacctggcga cccaatgtcg cctattttga
    16621 aggcgacaat gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg cttgattgtt
    16681 atccctgcgg ctggttactc accagccgcg acatcgtctc cagcgaatcc gttcttaaga
    16741 tataaagtcg ttttagtaac aacggattat cccccggttt tactttgcct ttcatggttg
    16801 tcaccgccag gtgaaatccg gcatcgtttg ctgccttcac ggctttgtca ttaaatccgc
    16861 caaacggata cgaaagatac cagacatgcg gattaaattg cgccagagcg cggcgtgaac
    16921 gtgcaaaatc aaacagaata ttgtgctcac tacggctcag taatatgggt cggcgatagc
    16981 catccacccg atgcaaaaaa tgggtatgtg actgaaaatc aaaaacatcg cgaatttcgt
    17041 taagctcaga aacgctcata aattgcagcg attttgggtt ccacttctgc gggtgacgtt
    17101 tgatgcgcga ggtaacaaca aacgccgtcg ccttcatgcc atattgtttc aatacaggat
    17161 acgcatagcg gctcaccgac ttgaggccat catcaaaggt aatcaccacc gctcgcgcag
    17221 ggagattgat cttattcttc acgtaacctt ccagctgcgc catgctcagt gtcgcgtatc
    17281 ccctgtcgcg cagccaggcc atctggttat tgaaagcgcg taccgaggtg gtcgtcgaag
    17341 tatggcgaaa acgggtgttt tcttcatcgc gcagaatatg gtgataggtt agcaccggca
    17401 ggccattatc gggttgggca tccagcgcgc tgatataggc cagtcgatcg ccaatacgga
    17461 tctgatacca ggtttgattt aacctgtctt tcagtttatg caaaatcggg tagcgcaaat
    17521 tgtccgccag taccccaaat ggcgcactgc ccgcactcgg cgcgttatag accggcgtat
    17581 ctttccaggt aattaagttc tgattactca gcggcttgtt gaggtcaccc aaaccgtctt
    17641 caactttttg tcgcccctga actggctcga gatgcccttt atcgataaac cctttaccaa
    17701 agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg atttgcccgg
    17761 cccgaatatt ccccacggtc accatcttgt cgccaatttg cgcccagatc gcagcatttt
    17821 cgatggtttg catataacgg gcaggtaacg cggcactgac acttgcggta aacagcatca
    17881 gcaggagaat aacagcttgt ttgtacatga aaaaataacc gagggtgaaa caacgctttt
    17941 attgtagcaa aagcgctata agaatcgtgg gttaatactc atacaaatta tgtttgagga
    18001 cgaaaggcgg atttttttgt tgttggtgcc aaagactact cacctccggc ccacccagtt
    18061 cgacaatacg ctcgcgaaac tctgaactgg tcatggtttc acggcaagcc atcatctgtt
    18121 caattaacgg taacgtgaca ccagaaatca cttcgcaacg ggagtgtttg tggcttaata
    18181 atgaagccac gcggtacggt ggcgctccgg ctatatcggt taagaagatg acaccctcac
    18241 cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct gagcatatta gagcttaacc
    18301 cacgccagaa attcacggcc cggcactgaa gaagcgcccc atgttttttc tccagcgcat
    18361 ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac atcgccttcc
    18421 tccttagccg aaaatcagtc taccctgacc attgttgata aagggtgata gccgtcaaaa
    18481 ctcctctccc ccaaatccag aggagaggaa atagccttag ctacgcaaac cacgtccacg
    18541 ttggatcagc gaccaacaga tcaaataaaa cgccacaata aagaccacca gtacgccaaa
    18601 ggtagtgacc agcggaacat cattgatacc gaggaagccg tagcggaaac cactgatcat
    18661 ataaacgatt gggttcaggt gcgacagccc ttgccagaac ggcggcaaca aagtcagtga
    18721 gtaaaagacc ccacccaaat acgtgagtgg cgttaacaca aaggttggca ccaggctgat
    18781 gtcatcgaac gttttggcaa acacaccgtt cagcaaaccc gcaagggaga acaacaccgc
    18841 cgtgagcacc agggttaagg caacgaatac ccacgaatgc acctgaaacg gcacaaaaaa
    18901 cagtgaaatt gccgtcacca gaatgccaac aaacagacca cgcgccacgc caccgccgac
    18961 atatccggca ataatgacgt gagtcggaac cggcgctacc agcagctctt caatattacg
    19021 ctggaacttg gcaccaaaaa atgatgacgc aacgttggcg taggcattgg tgatcaccga
    19081 catcatgatc agccccggta cgatgaactg catatagctg aagccatgca tatcgccaat
    19141 acgcgaacca atcaggttac cgaagataat aaagtaaagg gtcatggtga tgactggcgg
    19201 caccagcgtc tgcacccaga tacgcataaa gcgatggatc tctttcgccc agatgctttt
    19261 tagcgccacc cagtaaagat gcatcatgcg cgatctcctt gtttttcatt aaccagtgaa
    19321 acaaacagct cttccagacg gttagctttg ttacgcatac ttaatacctg aatgccctgc
    19381 tcacttaact gcgtaaatac gctgttgatc ccctgctcac gtagcacttc aacttccagc
    19441 gtcgcggtat cgaccagtcg atactgatag ccatcgagct tcggtaacgg acttttcggt
    19501 gcgagatcga gaataaaggt ttccgatttc agcttcgcca gcagcgcctt catcgaggta
    19561 ttttccacca gctcaccgtg ttgaataatg ccgatattgc ggcacagcat ttctgcttct
    19621 tccaggtagt gtgtagtgag aatgatggtg gtgcctttgt cgtttaaatc cttcaaaaag
    19681 ccccacattg agcggcgaag ttcaatatcc acgcctgcgg tcggttcgtc gagaatcagt
    19741 agtttaggtt catgcattaa cgcacgggca atcattaaac ggcgcttcat cccgccagat
    19801 aacatacgcg cacgttcgtt gcgttttccc catagatcga gttgtttaag atatttttcg
    19861 ctgcggatgt acgcctcttt gcgctccaca ccgtagtacc ctgcctgatt taccacaatt
    19921 tgctgcacgg tttcaaacgg gttgaagtta aattcctgcg gcaccagtcc caactgacgt
    19981 ttagcgttca cgacatcttt ctcgagatcg taaccaaata cgctgacccg cccggaggtt
    20041 ttatttacca gagagctgat aataccgata gtggtcgatt tcccggcccc gttcggcccg
    20101 agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcta ttccacgaag cgcctgaacg
    20161 ccgcctggat aagttttttt aagctgttga agttccagtg caatggtcat aaatttttac
    20221 ttaccttacg ttcttacact ttatctgtgg tttaaatcgt cccggagttg ccctatatta
    20281 gccaaacgta attatttggt tacaggtcgt taacctccat gaaagacata gatacactca
    20341 tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg ttttttgaga
    20401 aactggcaca agcgcaaaaa ccgcgctttc tatggattgg atgttccgac agtcgcgttc
    20461 ctgcagaacg tttaaccggt cttgagccgg gcgaactctt tgttcaccgt aatgttgcta
    20521 acctggtcat tcacaccgac ctgaactgcc tttccgtggt tcagtatgca gtggatgtac
    20581 tcgaagttga acacattatt atctgtggcc actacggttg cggcggcgta caagccgcag
    20641 ttgaaaaccc ggaactgggg cttatcaaca actggctgct gcatatccgc gatatctggt
    20701 tcaaacatag ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat accttgtgtg
    20761 aactgaacgt catggaacag gtttataacc tgggccactc caccattatg caatcagcgt
    20821 ggaaacgcgg gcagaaagtt accattcacg gctgggccta cggcattcac gacggcttgc
    20881 tgcgtgatct ggatgtcaca gcgaccaacc gcgaaaccct tgagcaacgt taccgtcacg
    20941 ggatttccaa cctcaagctg aaacacgcca accacaaata aaaatgccat gccggatgca
    21001 acacatccgg caacttcaca cttactcgtc cagcagaatc actttgccga tatacggcag
    21061 atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat ccgggatcga
    21121 gaaaccgata aattcaaccg ggacgttcac ttcacgacgg gacggtttat ccagcagcgt
    21181 acaaatcgcc agcgacttcg gttcgcgcag gcttaagatc tcacgcactt tcgacagtgt
    21241 attccccgag tcgatgatat cttcaacaat cagcacgtcc ttgccacgga tatcttcatc
    21301 cagatctttg aggattttca catcacgggt ggtggacatg ccgctaccgt agctggaggc
    21361 ggtcataaag tcgacttcat gagatacctg aacttcacgg cacaggtccg ccataaacat
    21421 aaatgagcca cgcagcagac caaccagcac catatcgctg ccgctgtctt tgtaacgctc
    21481 agtaatctga cgacccagtt cggcgatacg cgctttaatc tccgcttcgg ggatcattac
    21541 ttctacagta tgtttcatat ctctaaccat atgatttaaa aacaaatcac ttcgcacggg
    21601 caggttttgg caccgcaaca aagcgcaaga cacgcagtat accagtaaaa ctattcattc
    21661 gcggaatctg ttaataaagc tcgaattgtg atgacgatca cacatgttaa aacctataat
    21721 taattgctac taaaatatta atgaattgaa atggtgtctc tttatggcaa ttaacaatac
    21781 aggctcgcga cgattactcg tcacgctaac agcccttttt gcagcgcttt gcgggctgta
    21841 tctactcatt ggcggaggct ggctggtcgc gattggcggc tcctggtact accctatcgc
    21901 tggtcttgtg atgctcggcg tcgcctggat gctgtggcgc agtaaacgtg ccgcgctttg
    21961 gctgtacgca gccctgctgc tcggcaccat gatttggggc gtctgggaag ttggtttcga
    22021 cttctgggcg ctgactccgc gcagcgacat tctggtcttc ttcggcatct ggctgatcct
    22081 gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg cactggtggt
    22141 ggcactgctg attagcggtg gtatcctgac ctgggccgga tttaacgatc cgcaggaaat
    22201 caacggcacc ttaagcgcca atgccacacc tgcggaagct atctcacccg ttgccgatca
    22261 ggactggcct gcctatggtc gcaatcagga aggtcaacgc ttttcacctc tgaaacaaat
    22321 taacgccgat aacgtccata atctgaaaga agcctgggtg ttccgtactg gcgatgtgaa
    22381 gcagccgaac gatccgggcg aaatcaccaa tgaagtgacg ccgattaaag tgggcgacac
    22441 cctttacctg tgtaccgctc accagcgcct gtttgcgctc gatgccgcca gcggcaaaga
    22501 gaaatggcat tacgatcctg agctgaaaac taacgagtct ttccagcacg taacctgccg
    22561 tggcgtctct tatcatgaag ccaaagcaga aacggcttcg ccggaagtga tggcggattg
    22621 cccgcgtcgt atcattcttc cggtcaatga tggtcgactg attgcgatta acgctgaaaa
    22681 cggcaaactg tgcgaaacct tcgccaataa aggcgtactc aatctgcaaa gcaatatgcc
    22741 agacaccaaa ccgggtctgt atgaaccgac ttcgccaccg attatcaccg ataaaaccat
    22801 cgtgatggcc ggttcagtta ccgataactt ctcaacccgc gaaacgtctg gcgtgatccg
    22861 tggttttgat gtcaacaccg gggagctgct gtgggctttt gatcccggcg cgaaagatcc
    22921 gaacgcaatc ccgtctgacg aacacacctt tacctttaac tcgccaaact cctgggcacc
    22981 agcggcctat gacgcgaagc tggatctggt ctatctgccg atgggcgtga ccacgccgga
    23041 tatctggggc ggtaaccgca caccggaaca ggaacgttat gccagctcga ttctggcgct
    23101 gaatgccact accgggaaac tggcgtggag ctaccagacc gttcaccacg acctgtggga
    23161 catggatctt ccggcacagc cgacgctggc ggacatcacc gttaatggtc agaaagtgcc
    23221 agttatttac gctccggcga aaaccggcaa catttttgtg ctcgatcgtc gtaatggcga
    23281 actggtggtt ccggcaccgg aaaaaccggt tccccaaggt gcagcgaaag gcgattacgt
    23341 aaccccaact caaccgtttt ctgaacttag cttccgtccg acgaaagatt tgagcggtgc
    23401 ggatatgtgg ggagccacca tgtttgacca gctggtgtgc cgcgtgatgt tccaccagat
    23461 gcgctatgaa ggcattttca ccccgccatc tgaacagggt acgctggtct tcccgggtaa
    23521 cctggggatg ttcgaatggg gcgggatttc cgttgatcca aatcgtgaag tggcaattgc
    23581 caacccaatg gcactgccgt ttgtttcgaa actgatccca cgcggtccgg gtaacccgat
    23641 ggagcagccg aaagatgcca aaggcacggg tacggaatcc ggcattcagc cacagtacgg
    23701 tgtaccgtat ggtgtcacgc tcaacccgtt cctctcaccg tttggtctgc cgtgtaaaca
    23761 gccagcatgg ggttatatct cggcgctgga tctgaaaacc aatgaagtgg tgtggaagaa
    23821 acgtattggt acgccgcaag acagtatgcc gttcccgatg cctgtaccgg tgccgttcaa
    23881 tatgggtatg ccgatgctgg gcgggccaat ctccacggcg ggtaacgtgc tgtttatcgc
    23941 tgctacggca gataactacc tgcgcgctta caacatgagc aacggtgaaa aactgtggca
    24001 gggccgttta ccagcgggtg gtcaggcgac gccaatgacc tatgaagtga atggcaagca
    24061 gtatgtggtg atctccgcag gcggtcacgg ttcatttggt acgaagatgg gcgactatat
    24121 tgtggcttat gcgctgccgg atgatgtgaa gtaaccataa tgcccggaga aatccgggca
    24181 tatttccgaa tacggtcttt ttataccgta aaccctaaca tcatccccgt atcttcatgc
    24241 tccagcagat ggcagtgcgc catataagca tgttctttcg gtgcgtcgtg attaaacttc
    24301 accagcactt cactgacatt gccttctacc ttaacggtat ctttccagcc cgcgcgatga
    24361 gccgctggcg gtttgccatt ttctgacaag atacggaact gggtgccgtg aatatggaac
    24421 ggatgcagca tcatgtcgcc cacgccagag ataacccaac gttcgtactg ccctttcgcc
    24481 gccgcaaaca tcggcttatt catatcaaac gcctgaccgt tgattttatt ggcatggtgg
    24541 aaatcgaact tcccaccgtg gttcatatga ttcatattac cgtgccccat atggcccatc
    24601 atctggctgt gatccattcc ggccatcgcc tgatcgccat atttctccat tagcatctgc
    24661 atacccatca tatcgagcat cgggtccata gaaagttgca gcttgcgtac cgtcagccct
    24721 tccagcgaag gtaacgcagg caggctactt aatgtgtctg gcaaagcacc ggaggcacta
    24781 atagcaatcg gctgaatccg cattaccgga tgcggcttgt caaacggcgc aatcgccatc
    24841 cccatctggc tgaccggtag cgtcaccagg tcaaagggtt tgttgtcgtt aacctccacc
    24901 agcacttcaa aacgctcgcc catcagcacc ggcagctcgc tcaccttcac cggttcaggt
    24961 agcagaccac cgtcgctggc aatcacatac agcgggcgat tgtcgctggt ggcgaaattg
    25021 agcgagcggg cattacagcc attgagcaaa cgcaggcgca gccaaccacg cggggcagcg
    25081 tgttgtgggt agattgcacc gttggtcagc agcgtatcgc caaaccagcc cacggcggcg
    25141 gtcatcacat ccagttgata atcaatctgc ccatcagcgt taaatttttt atcctgaaca
    25201 atcaccggaa catcatcgat accccactgt tttggcagca ttaatttcag gatctcgtca
    25261 tcttcaatta ccaccagccc agccaacccc atcgccacct gtcgcccggt cttgccatgt
    25321 tgatgtggat ggaaccagca agtagcggca ggttgatcaa cgttcaacgt caccgagcgc
    25381 ttgccacctg gcagaataat tccctgcggg ccgccgtcga cttcacccgg tacttccagc
    25441 ccgtgccagt gcaacgtcgt ctcttccgtc agttggttat agatatcaac cgttaccgct
    25501 ttgccgcgct gtaatttcac cgccggcccc agcagattgc cgttatagcc ccaggtagtt
    25561 gcagttttct cgccaaaggt agactgacct gcgccaatag ttaactgaat gcgattacgg
    25621 gcatcggtcg tgagcaaatc agggatcggt aacgttgggc gttccgccgc aaagactgcg
    25681 cggctccaca gcggcaaggc tgaagccaca cccagcgcga cggaatattt taagaaatca
    25741 cgacgttgca tagttatttc cttattctta agcaggcgaa acaaaatcaa acgttgagca
    25801 tagtccttcc ccttacggga aggtcaagcc gcagacacaa tttaatcgac aataatggtc
    25861 gtcgcctcgc tcgcagtgtg ctaacgttta tcttctttaa gcccctggta gaagcaatga
    25921 agacgttttt cagaacagtg ttattcggca gcctgatggc cgtttgcgca aacagttacg
    25981 cgctcagcga gtctgaagcc gaagatatgg ccgatttaac ggcagttttt gtctttctga
    26041 agaacgattg tggttaccag aacttaccta acgggcaaat tcgtcgcgca ctggtctttt
    26101 tcgcccagca aaaccagtgg gatctcagta attacgacac cttcgacatg aaagccctcg
    26161 gtgaagacag ctaccgcgat ctcagcggca ttggcattcc cgtcgctaaa aagtgcaaag
    26221 ccctggcccg cgattcctta agcctgcttg cctacgtcaa ataatccctg ataccttttt
    26281 gtagaaatat tgaccgtgca tccgcggtca atgttagcta ttatgttgcg cccttttttt
    26341 acgggtgtta acaaaggagg tatcaaccca tggccgaaaa aaaacagtgg catgaaacgc
    26401 tacacgacca gtttgggcag tactttgcgg tagataacgt tctgtatcat gaaaagaccg
    26461 atcaccagga tctgatcatt tttgagaacg ctgcatttgg tcgcgtaatg gcgctggatg
    26521 gcgtagtaca aaccaccgag cgcgacgagt ttatctatca tgagatgatg acccatgttc
    26581 cgctactggc ccatggtcac gcgaaacatg tgctgattat cggcggcggc gacggtgcca
    26641 tgctgcgtga agtaacccga cataaaaacg ttgagtcaat cacgatggtg gaaatcgatg
    26701 cgggtgtcgt atcgttctgc cgtcagtatc tacccaacca taacgccggt agctacgacg
    26761 atccgcgctt taagctggtg atcgacgatg gcgtcaattt cgttaatcaa accagccaga
    26821 cctttgatgt cattatctcc gactgcaccg atcctatcgg tcccggcgaa agccttttca
    26881 cttcggcatt ttatgaaggc tgcaaacgtt gcctgaatcc tggcggtatc ttcgtcgcac
    26941 aaaacggcgt ctgcttttta cagcaggaag aagccatcga cagccatcgc aaactcagcc
    27001 attacttcag cgacgttggc ttttatcagg cggcgatccc gacctattac ggcggtatca
    27061 tgacttttgc atgggcgaca gataacgacg ccttacgcca tctctcaacc gaaattattc
    27121 aggcgcgttt tctcgcctct ggcctgaaat gccgttatta caatccggca atccatacgg
    27181 cagcttttgc cttacctcag tatctgcaag acgcactggc ttcacagccg tcctaaggag
    27241 aagataagaa attgaaaaaa ctgaaactgc atggctttaa taatctgacc aaaagtctga
    27301 gtttttgtat ttacgatatc tgctacgcca aaactgccga agagcgcgac ggttatattg
    27361 cttatatcga tgaactctat aatgccaacc gtctgaccga aatcctgtca gaaacctgtt
    27421 ccattatcgg ggccaatatt cttaacatcg cccgccagga ttacgaacca cagggtgcca
    27481 gcgtcactat tctggtgagt gaagaaccgg ttgacccgaa actcatcgac aaaacagaac
    27541 accccggccc actgccagaa acggtcgttg cccatctcga taaaagtcat atttgcgtac
    27601 atacctaccc ggaaagtcat cctgaaggcg gtttatgtac cttccgcgcc gatattgaag
    27661 tctctacctg cggcgtgatt tctccgctga aggcgctgaa ttacctgatc caccagcttg
    27721 agtccgatat cgtaaccatt gattatcgcg tgcgcggttt tacccgcgac attaacggta
    27781 tgaagcactt tatcgaccat gagattaatt cgattcagaa ctttatgtct gacgatatga
    27841 aggcgctgta tgacatggtg gatgtgaacg tctatcagga aaatatcttc cataccaaga
    27901 tgttgcttaa agagttcgac cttaagcact acatgttcca caccaaaccg gaagatttaa
    27961 ccgacagcga gcgccaggaa attaccgctg cgctgtggaa agaaatgcgc gagatttatt
    28021 acgggcgcaa tatgccagct gtttaacggc tctggcggag ctcccaggct ccgccagatt
    28081 tatttacttc tgctgcacga aattgcggta agccgccacg acctgcagaa aatcctcaac
    28141 gccgcatagc gacaggcttt cttcgtcgta gtagttcatc ccctcttcca tttcatcgcc
    28201 agcgaattcc agttgattgg cgcgaaccat cacctcttca ccgtccatcc acagggtgta
    28261 ttcatgccct gcccgttgcc aggaccgttc gctacctttc agtgcgtgcg ctgcttgttc
    28321 cacttcatca agcaaggcca ggttttcttt cacctcttca ttaaaccagt gcccgaccac
    28381 ttcatgcccc atggacatac gcacctttac cactccggta atatcgcgca gaaattcgta
    28441 atccatagtg tgttcctctg ctcccggcaa tgcgccgaaa tcgcatcgcg ccattgatgt
    28501 aattatcgca gccttaccgg agaaaaacag cggaacggcg atggtttaga aataaaaaat
    28561 gcccgggaat ctcattcacc gggcattgtg tcgtttatgc gcagcgcgtg cgcttactta
    28621 ttacaccgca gtctggaaaa tcaccccatc ggctttttcg gtgtactgag aaagctggtt
    28681 gaagttcaga taacgataag tatcaacggc agttttatct acttgcgcaa cgtaggtctg
    28741 gtactcttcc ggcgtcggca gtttgccaat cagcgccgca acagccgcca gttctgcaga
    28801 agccaggaag acattcgcgc cagtccccag acggttcggg aa
//
LOCUS       DAEVZA010000052        28397 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEVZA010000052 DAEVZA010000000
VERSION     DAEVZA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.052"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..148)
                     /locus_tag="LAF92_002335"
     CDS             complement(<1..148)
                     /locus_tag="LAF92_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791334.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRL"
     gene            complement(324..3692)
                     /gene="recC"
                     /locus_tag="LAF92_002336"
     CDS             complement(324..3692)
                     /gene="recC"
                     /locus_tag="LAF92_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBI9791335.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(3705..4028)
                     /locus_tag="LAF92_002337"
     CDS             complement(3705..4028)
                     /locus_tag="LAF92_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBI9791336.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(4013..4420)
                     /locus_tag="LAF92_002338"
     CDS             complement(4013..4420)
                     /locus_tag="LAF92_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBI9791337.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(4417..4980)
                     /locus_tag="LAF92_002339"
     CDS             complement(4417..4980)
                     /locus_tag="LAF92_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBI9791338.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTGTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(4971..5441)
                     /locus_tag="LAF92_002340"
     CDS             complement(4971..5441)
                     /locus_tag="LAF92_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBI9791339.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(5626..6420)
                     /gene="thyA"
                     /locus_tag="LAF92_002341"
     CDS             complement(5626..6420)
                     /gene="thyA"
                     /locus_tag="LAF92_002341"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBI9791340.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(6427..7302)
                     /gene="lgt"
                     /locus_tag="LAF92_002342"
     CDS             complement(6427..7302)
                     /gene="lgt"
                     /locus_tag="LAF92_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBI9791341.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(7453..9699)
                     /gene="ptsP"
                     /locus_tag="LAF92_002343"
     CDS             complement(7453..9699)
                     /gene="ptsP"
                     /locus_tag="LAF92_002343"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI9791342.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(9712..10242)
                     /gene="rppH"
                     /locus_tag="LAF92_002344"
     CDS             complement(9712..10242)
                     /gene="rppH"
                     /locus_tag="LAF92_002344"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBI9791343.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            10927..11616
                     /gene="mutH"
                     /locus_tag="LAF92_002345"
     CDS             10927..11616
                     /gene="mutH"
                     /locus_tag="LAF92_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBI9791344.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALVGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            11685..12398
                     /locus_tag="LAF92_002346"
     CDS             11685..12398
                     /locus_tag="LAF92_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBI9791345.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            12536..12754
                     /gene="ygdR"
                     /locus_tag="LAF92_002347"
     CDS             12536..12754
                     /gene="ygdR"
                     /locus_tag="LAF92_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBI9791346.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            12862..13902
                     /locus_tag="LAF92_002348"
     CDS             12862..13902
                     /locus_tag="LAF92_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBI9791347.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(13934..15127)
                     /gene="lplT"
                     /locus_tag="LAF92_002349"
     CDS             complement(13934..15127)
                     /gene="lplT"
                     /locus_tag="LAF92_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBI9791348.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(15120..17279)
                     /gene="aas"
                     /locus_tag="LAF92_002350"
     CDS             complement(15120..17279)
                     /gene="aas"
                     /locus_tag="LAF92_002350"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBI9791349.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTLLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            17865..18896
                     /gene="galR"
                     /locus_tag="LAF92_002351"
     CDS             17865..18896
                     /gene="galR"
                     /locus_tag="LAF92_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBI9791350.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTDNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(18903..20165)
                     /gene="lysA"
                     /locus_tag="LAF92_002352"
     CDS             complement(18903..20165)
                     /gene="lysA"
                     /locus_tag="LAF92_002352"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBI9791351.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            20287..21222
                     /gene="lysR"
                     /locus_tag="LAF92_002353"
     CDS             20287..21222
                     /gene="lysR"
                     /locus_tag="LAF92_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBI9791352.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATIA"
     gene            complement(21209..21901)
                     /gene="ygeA"
                     /locus_tag="LAF92_002354"
     CDS             complement(21209..21901)
                     /gene="ygeA"
                     /locus_tag="LAF92_002354"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBI9791353.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(22030..23448)
                     /gene="araE"
                     /locus_tag="LAF92_002355"
     CDS             complement(22030..23448)
                     /gene="araE"
                     /locus_tag="LAF92_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBI9791354.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWFIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(23763..24524)
                     /gene="kduD"
                     /locus_tag="LAF92_002356"
     CDS             complement(23763..24524)
                     /gene="kduD"
                     /locus_tag="LAF92_002356"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBI9791355.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(24554..25390)
                     /gene="kduI"
                     /locus_tag="LAF92_002357"
     CDS             complement(24554..25390)
                     /gene="kduI"
                     /locus_tag="LAF92_002357"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBI9791356.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(25677..26858)
                     /locus_tag="LAF92_002358"
     CDS             complement(25677..26858)
                     /locus_tag="LAF92_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBI9791357.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            27113..28342
                     /locus_tag="LAF92_002359"
     CDS             27113..28342
                     /locus_tag="LAF92_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBI9791358.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         7175 a         7651 c         6844 g         6727 t
ORIGIN      
        1 ccagacgtat agcctgatac tggcggttat ctttatcact tttacggatg gtttcctgaa
       61 tcggctgcca tcccgtttct gcctgactta agggtgccca aagggcaaca aacaacaata
      121 atgctttgaa ccaggtgctg cggggcattc acggacctca taatcaactt tattttctgg
      181 ccagattcaa caggttaacg caaatcatct gccagaattt aatcttgtgc tgcacgagtc
      241 agcctatgtc tatatatcca tcagtccgtg actggtgcgc atcataatgt aagcgaatga
      301 attgcgcaat ttttatacag cactcatgac tgattaaagc gaaacagcgg taacaggaaa
      361 cgttgcgact gttcaacgat ggcctccatt gtctctggtg ttaattgccg ccagagccgt
      421 tgataccaga tatcatcacc ttcgccacgc accatcatgt tgccttcgta agcctgaagg
      481 aatttcgtac gggctttttg caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg
      541 tcataacagg tttttagcca cgcgccgcca ctttcaggta acaccagcaa tggcgcggac
      601 attccttcac gatacccctc aatcagttgt gagaggtaat gcaaagcctg ttcggctgca
      661 agcggcggaa aacgccactc accgtctttg cgtagaaaaa ggcgactttc gccattacca
      721 ccgctggcac agtagacaag gtgttccagc caaagttgca ttccctgcgc cacacttaat
      781 aaagagggac gccagcgcaa caggccatcc ggctgcacct gcggcaacca gccagttatc
      841 tgcacaccgt tgcaggcgag atcaatttcc atactctgcc ccggctggcg acaggcaatg
      901 actctgtcgg caagctgctg catctcctgg cactgtgttt cccagaaaat ttcaccaaaa
      961 gcgccatacg gtaaatcccc tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc
     1021 tgctcaacca gtgcattcaa taactgctga ttgatttgat aacggctaag tccttccaga
     1081 ataaatggct cggtgtctgg gatttcgctg tcttcagtac ggaagttcac ctgcaaacgc
     1141 atctggaaga acgcccgcac cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg
     1201 gtttccggta aggtaaacgg cagcggctga acaaattcag aatgtgcttt accagcctgg
     1261 ctggccgcag gtagccattc acgagcatag ctttgtcgtt cgcctggctg gtagttttgt
     1321 ggatcaaacg gcatccgggt atggaggcaa gtaagatgcg cttttaccct tgcctcgctt
     1381 tcatcacagt tgagcgcttc atcgcccggt agataatgac tttgcccgat gtagtcgatc
     1441 agttcctgca ccagtaccga cgggaaacgt tcactgttat cctgaatgga acgcccgata
     1501 tagctgatat agagtttttg ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg
     1561 tcatcgcgac ggctacggtc gccacgcttc ggtttctggc tcatcaggtc aaagcccaat
     1621 ggcgcaagct gacgtggata aacgccgtcg ttcattccca gcaggcaaac cactttgaac
     1681 ggaattgaac gcattggcat cagagtacaa atgttaaccg gtccggcgag aaaacgctgg
     1741 ctgatacgtt cttgatccag acgctgcgcc agttcatcac gcaatagtga cagcggcacc
     1801 gcgtcgccat actgcgcacc taaaccttcg gcgataatcg cctgccattg ttgttcgatc
     1861 agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga aggcgttgag catatcgcga
     1921 caaaccggca accactcttc tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt
     1981 agctgcatta gcagtgaagc cagatgcccc accagttctg caattaagcc gctcgattca
     2041 tcataaggta gaaccgattg ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac
     2101 atacgcgtca ggccaaatcg ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga
     2161 acgttgtcgt catctatccc ccaacgaatg ccggattcgt tgacccactg gcgtaaataa
     2221 cgcagccctt cttcggtgat gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc
     2281 agcacatcct ctgacacaaa gcgactgtca ggcagtgata acaggctgat aaacgcttcc
     2341 agtaccggat gtgactgccg cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc
     2401 gcaggtgcgc taccaaacac tgcctgaata aacggactgt agctgtcgat atcagccacc
     2461 atcacgatga tgtcgcgcgg agtaagtgtc gggtcttcct ccagcatcgc cagcaagcga
     2521 tcgtgtaaaa cttcaacttc acgctgcggg ctatggcaaa cgtggaatgt gatactgcta
     2581 tccagtggat caagcgggcg tttgttatcg ctacgggaaa actcttcgat gttcacaccc
     2641 gcaacggcgc ggttttccag ttccagaatg tcagactgaa tgttatgcag caggttatct
     2701 ggcgtgacat cgacaaaggc gtccagctcc tggctgctct ccaggtcaga aaggagataa
     2761 atgtagtcgc gcccaagctt accccatgaa gccagcagcg ggttgccgac atcctgttca
     2821 ccatcgctgt taaagagctg cccggcattt tcgctatcac gaaatagcgg caattcgcga
     2881 tcttcaaaac tgtgtcgacg ctgacgggtc agcagtttcg ccagataagc aggatcttta
     2941 atatcgcccc agtaataacg gcaggggttg gtaaacagga gatggatttc aatatgttta
     3001 cccagcgcct ggagcgcctg gagataaaca ggcggtaacg cggaaatacc gcatataaag
     3061 acgcgcgaag gtaaccccgg cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc
     3121 tgatagagat tggcgcggtg ccagcgcggt tgtccgagtt catgggtata ttccaccagc
     3181 gccttccaca acggcgcttg ccaggcctgt gcttctccca gcccttcaac caaatgtcct
     3241 gtttcccatt gtgccagcca ctccgggcga tagaccagat actggtcaaa caggtccgcc
     3301 gcttttgagg aaagctggaa cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc
     3361 aacagggtaa agtcttcgcg ctccagcaat tgcggcagca gagtcatcag tttccagctc
     3421 atgctctgtt tgttaaaggc gctctctttg gggatttccg gtaacacccg gacgaacata
     3481 tcccagataa agctcgctgg cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc
     3541 gacagggtca tttgcagcca ctgcgccata ccggtacttt gcaccagaat catctctggt
     3601 tcgaaaggat cgtccagccg ttcgcgttcg acaataaact ccatcaacgc ttccagcacg
     3661 tccagacgat tggaatggta gacccttaac atagcggctc ctgactactg acgattcggg
     3721 caatgcaggc gggtcatctc gccttctctg cccccgggtg aaactagcgt aacgctgatg
     3781 ctgacacatc ccgcctgcga tgtctgcatt cggttgacct gccagttggc aggtggcgaa
     3841 atcgcgcgca gttgcgtttg ctgccaacca tgccgccaga gctgttggta ctggtttctg
     3901 ctggcgagac tgttcattaa tgttcgctgg atacccgata acgcagtgac aatcatcacc
     3961 atcagcacca tcgcaaccat tacctccggc aggctaaagc cttgttgatt cttcagggaa
     4021 gctgacataa tgccccctct ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa
     4081 caatttttcc atcaatgact tcgcctgttc gccacaacga aacgccttca taaccggcaa
     4141 tcaataaggc ttcattatct gccagtaaac gcaaacaaac ctgggcatcg gttccagcgt
     4201 actgcgagca ctgaactgct ggctgcgtct gccaggagtg catttttccc cacgccagcg
     4261 ccgactgaac gatggcctgg cggcgcaatg actgactttc catgctcacg cgagaggcaa
     4321 aactgcgatc ctgctgactc attccttgca atagcaagct acccaaaacc agcagcatca
     4381 ggaccagagc cagtgacgaa acaccttttt cgcggttcac aggttgaatc ctgtcacgct
     4441 atagctggca tccaccacgg tttgcggttc agacttactg gcagcacgca tattaaccgt
     4501 caacaccggc gagaagccgc tgacatcctg acgtacgacc tgaaaagtgt cgataatgat
     4561 ggcatccgga ttagtgactt tatcccagcc cttaccttca caggatgtcg caccgcgtag
     4621 cgtttccagc acatgctcct tcagacgaaa tccaatctgg tcggactctt ttaccggttc
     4681 gcgatcccag ataccgttac tgttcgcatc ccactgcaca atgatacagt caccctgtcc
     4741 gacaatttcc agcccttcgc cggtacagct gccatgacaa taacccgccc tctggagatg
     4801 cttcgcgacg gtaaataccc gcagccagat ttcatcttcc agcgccagct tacgggtgcc
     4861 cgttaaactt tcacgctgta acgcaggcag aaagcgtgcc gctcccagca acaatacgct
     4921 actgatcgcc atagcaatca acacttccag cagagaaaaa ccttgctctt ttacaggcat
     4981 ccttctgttt ctccttgctg acaaagccgg agtcttcccc acggcgaaac caccagccac
     5041 cactcgcccg ttgagttttt gaagcgaata tgcccggccc atgcggtatt gcgcaggcca
     5101 aagaaagcaa gcgaaggtgt caggtcgctc atttcgactt cgggccagcg tggcacaaag
     5161 accaatggtg aactgccatg acaggtattc gccccagcaa cggaactcac aaggcaccat
     5221 aacgtcccct ctctgataac actgatactg tggtcgcggt tatgccagtt ggcatcttca
     5281 cgtaaataga gcaaatagtc ccgcgcctgg ctggcggttt gccaaagccg ttgcgactgc
     5341 tgccagtatt gccagccata gagtccactt gcgcttagca tgaccagaat cagcatcgcg
     5401 accagcgttt caatcagcgt ataaccacgt tgtgttttca tgccggcagt atggagcgag
     5461 gagaaaaaaa gacgagggcc agtttctatt tcttcggcgc atcttccgga ctatttacgc
     5521 cgttgcagga cgttgcaaaa tttcgggaag gcgtctcgaa gaatttaatg gagggtaaaa
     5581 aaaaccgacg cacactagcg tcggctctgg caggatgttt cgtaattaga tagccaccgg
     5641 cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga aacggtagtc
     5701 gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg gttcgcggct
     5761 taattgcaga tgagtttgat ccatatgatt gctgtacagg tgcgtgtcgc cccccgtcca
     5821 gacaaaatcg cccacttcca gatcacactg ctgcgccatc atatgcacca gtaacgcgta
     5881 gctggcaatg ttaaacggca ggccgaggaa aacgtcacag gagcgctgat aaagctggca
     5941 agagagtttg ccgtctgcca catagaactg gaagaacgca tggcacggtg ccagcgccat
     6001 tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgcgaat ccgggtcgtt
     6061 tttcagctgg ttcagtaccg tagtgatctg gtcaatatga cgaccatccg gcgttggcca
     6121 ggcgcgccac tgtttaccat acactggccc gaggtcgccg ttttcatcgg cccattcgtc
     6181 ccagatggtg acattgtttt cgtgtagata agcaatgtta gtgtcgccct gcagaaacca
     6241 cagcagttca tggatgatgg aacgcaggtg gcaacgttta gttgtcacca gcgggaatcc
     6301 atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg ttccggtacg
     6361 gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat actgtttcat
     6421 ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca tcatgatcac
     6481 acccgcgaca atcatcggga tggaaagaat ttgtcccatg ctgatgtact gcacccaggc
     6541 accagtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa acgcgccgta
     6601 accaatcagg aacaaacctg agacagctcc cattgggcgc ggtttacgaa tatacaggtt
     6661 gaggataata aacagcacta caccttccag caacagctcg taaagctgtg atgggtggcg
     6721 cggcagcaca ccgtaagtat cgaaaatgga ttgccactgc gggttggttt gcagcagcaa
     6781 aatatcttct gtacgggagc cagggaacag catggcaaac gggaagttcg ggtcaacgcg
     6841 gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac caaacggaat
     6901 gagtggtgcg ataaaatcag agacctggaa gaaggaacgt ttagtacggc gggcgaagat
     6961 aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc cgtcccagac
     7021 acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca gaacataacc
     7081 aatacgtcca ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt caacttcatt
     7141 tttggtccag ccactgcccg gacgattcgc ccgtcgtgtt gccagccaca ttgcaaaaat
     7201 gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat
     7261 gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc acaagttctt
     7321 gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa
     7381 aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta aaagatatgt
     7441 atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac gctccataaa
     7501 ggctgcaacc tgatggcgaa cttcggtcgc cagttgcgct tccagactac gctgcgcaag
     7561 attttctgct tcggcataat caatgcgccg cagcaggtat tttacccgcg ctacagaacg
     7621 tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc acatgggatc
     7681 gcccgccatt tcaccgcaca aacggagatc gattccatgt atttccgctt cccgggcgat
     7741 catcgccaga gctcgtaaca ttgcaggatg aagactgtca taaatgttcg ccacccgggt
     7801 attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa cagagatgaa
     7861 atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt ccagcatgat
     7921 gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac gtccggcacg
     7981 ttcaatcagg cggcgtgctt cgtcaacttc atcgagactg gtgaccatcg gcaacagaat
     8041 attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga tcaagaagat
     8101 ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc aggcatggat tctcttcgct
     8161 gatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca aggtgacggg
     8221 tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt cttccgacgg
     8281 gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc catcaatacg
     8341 gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga cttttatacg
     8401 ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac ggctaagctc
     8461 aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg gatcgaccag
     8521 caattcaccg cgatagccat caacgatcag cgtcctacga tgcagcaccg aaggctgaat
     8581 atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag catgggagtt
     8641 ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct cagcaagagt
     8701 tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg
     8761 attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat cgccagcccg
     8821 ctctttgaga tagttatcgc ttagcgcggc aaactgttcg gcaaattttt caatgaccgt
     8881 ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag
     8941 ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc
     9001 accggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc
     9061 ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca cctgttccat
     9121 taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa
     9181 tgcgcggatt cgcgtctggc gatattgccc aaacaaggca gtcaactgcg actgagaaag
     9241 aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt cgtcatactg
     9301 gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt gaataattgg
     9361 tacgcctaaa aacgcgcgga aacgttcttc ttttacggag gggatgtatt tgaagctggg
     9421 gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca ggccgacgat
     9481 cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc
     9541 catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga cctcggtatc
     9601 catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc gtggtgcgct
     9661 ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga cttaacctct
     9721 tttacgtcga taagcagatg cgttttgtgg ttttggcgta ttttcctgca gtgacatcac
     9781 cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg acaccacctg
     9841 tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg tactgctggt
     9901 ttgcatattg atttctgcat cgccgctcac cagctgcaag agaaaccatt tttgtttttg
     9961 gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa
    10021 ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta cttcttcaaa
    10081 caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc cttgcggaaa
    10141 ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc gattacaaat
    10201 cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac ctcaaaataa
    10261 agctttatat acgaatgatt gtttcatact ccaggaagac ggtaaaccac tctctgcagg
    10321 gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta taaacagagg
    10381 aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga agaactacgg
    10441 aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat tttaaaatat
    10501 tcattattaa tcaatgcatt acatgtaatt tgagtaatga aaaagagtaa ttcgtgaccc
    10561 aggtcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat ttatccacag
    10621 aatgtgccac taagttaagc actgaaccac taaaaactgg agtttcgtcg cacgtcaagg
    10681 ctgtaaatgg aaacagtagt ggaggttttt cacagttatc ccagctttct gtggataaca
    10741 tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacccgttg cagtgttgcg
    10801 caactcgatt accggcaacc taaaaagcaa tataaatcag agaattgaac aacgcatgtg
    10861 gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta accaaggtat
    10921 catgacatgt cccaacctcg cccactgctc tctcctcccg aaactgaaga acagttgtta
    10981 gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact tgtcgggctg
    11041 gttacgccag agaatttaaa acgcgataaa ggctggattg gcgtgttact ggagatctgg
    11101 ctaggtgcca gcgcagggag taaacctgag caagattttg ctgctctggg cgtggaactt
    11161 aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt ttgtgttgcc
    11221 ccgttaacgg gcaatagcgg ggtgacctgg gaaaccagcc acgtgcgcca caaactcaaa
    11281 cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc cgctggcgca gcgtcgcgtt
    11341 ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc ggcagctacg cgaagactgg
    11401 gaagaattaa tggatatgat tgttctcggt caggttgagc ggattaccgc tcgtcacggg
    11461 gagtatttac agatacgacc gaaagcagcg aatgcgaaag cgcttaccga agccattggt
    11521 gcccggggcg aacggattct gacgctgcca cgcggctttt atttgaagaa gaatttcacc
    11581 agtgcgctac tggcccgtca ttttctgatc cagtagccat cgctttgacc tgccgctttc
    11641 cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta tttgcatgga
    11701 taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag atcgttcttg
    11761 ggatcgacaa tattattttc ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg
    11821 ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg ctgctggcat
    11881 caatcgcctg ggttacgcgc ctgacaaatc cgctttttac aatattcagt caggaaattt
    11941 ctgcccgtga tttgattctg cttctgggtg gcttgttcct tatctggaaa gccagcaagg
    12001 aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc
    12061 tcggcgctat cgtgcagatt atgctgctgg atattatctt cagcctcgac tcggtgatta
    12121 ccgctgtggg tctgtcagat cacctgttta ttatgatggc agccgtggtg attgccgtag
    12181 gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgaacgccat ccttcggtaa
    12241 aaatgctggc gctctctttc ctgattctgg tgggctttac cctgattctg gaaagtttcg
    12301 acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt gcggttgaaa
    12361 gcctcaacct gattcgcaac aaaaagaacc cgctctgata ctccgtacgc tctcctgcgg
    12421 gagggcgtac tcactcctgt taccctcaca gtttaagatt ttcctgcttt caagatatat
    12481 aacgtcagtt tataaacaga ctattatcat aggtgggcca aatgaggata accggatgaa
    12541 aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg ggtgttccag
    12601 tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa aacctgaaat
    12661 tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa
    12721 ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat ccggctggcc
    12781 ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc cttatgtcgt
    12841 gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc gctggaagtc
    12901 agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc cgacgcccac
    12961 gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc cgaaatgtac
    13021 ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg caactggctg
    13081 gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc
    13141 aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat ccgcgaagcg
    13201 ctgcatgaca gcctcaagcg cctacagact gattacctcg atctttatca agtgcactgg
    13261 ccacagcgcc caaccaactg cttcggcaaa ctcggttata gctggacaga ttctgcgcct
    13321 gcggtttcgc tgctggatac gctggacgcg ctggctgagt accaacgcgc gggaaaaatt
    13381 cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct gcatctggcg
    13441 gacaaacacg atctgccgcg tattgtcacc attcagaacc cctacagttt gttaaaccgc
    13501 agttttgaag taggtctggc agaagtcagc cagtatgaag gggtcgaact gctggcctat
    13561 tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa acccgctggc
    13621 gcacgtaata cgctctttag tcggttcacc cgctatagtg gtgagcaaac gcaaaaagcc
    13681 gtcgcggcgt atgttgatat cgccagacgt catggcctgg accctgctca aatggcgctc
    13741 gcatttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac cacgatggag
    13801 cagttgaaaa ctaacgtcga aagtttgcat ctggagttaa gcgaagacgt attagctgaa
    13861 attgaagcgg tacatcaggt ttatacttat ccggcaccat aagattaacg ccggttaaaa
    13921 ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt gccagcgcaa
    13981 atagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt accgccagcg
    14041 agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt actgcaatcg
    14101 cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg agcggaacga
    14161 caaaaaagcc ccccaacacg ccaatcagca tcaacaaggc ataggctggc agcagctcgt
    14221 gttgcaggga gaaaatcagt accaccacgc caatcaaaat cccggctggc atacagcgtg
    14281 acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg ataccaatcg
    14341 ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc gccaccggta
    14401 cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa ctggtgccca
    14461 ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc aggaaactgc
    14521 gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt ttgggaatgt
    14581 agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg gcgacgagga
    14641 catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc gctatggtag
    14701 aagcttccat taaaccgtta gcttttacta acttactacc cgtggttaat tcgccgagaa
    14761 taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta tagccgagaa
    14821 acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg ttggcaaaca
    14881 tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc gcaaaaagaa
    14941 tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga tagaactgcg
    15001 ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca gagagaaact
    15061 gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg tgcactgact
    15121 cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg
    15181 ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc
    15241 ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc gttatctgtg
    15301 gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt ggcatggact
    15361 ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac catttcgcct
    15421 gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa
    15481 cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac attctcggcg
    15541 gtaggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat tatgttcggc
    15601 cctttcagtt gcaggcgtcc gccctcttcg ataccaggaa ccgacaacag gcgcgcatcc
    15661 attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac gttgatagaa
    15721 acgacaggcg cgcactcggt tacaccatag ccttcaagga tgcgcaggcc aaatttatcc
    15781 tgccaaagct gtttggtact ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga
    15841 cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg
    15901 aacaacaggg tgcaactgcg gtcatacacc aactccggca caatgcggta atgcagcggg
    15961 cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc taccgtcagc
    16021 ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt gaagtcggca
    16081 attgttttaa tctgctcgac attcgccaga atgcttttat ggctatggac gacgcctttc
    16141 ggatggcctt cagaaccgga ggtaaaaagg atcagcgcct cttcttccgg ctgctgttta
    16201 acctgtgcca gacgcggcat cagcaaatga gcgaagatcc atactttgtc ggcagtggtg
    16261 acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg ctccggcaga
    16321 tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt gatttcagcc
    16381 gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat tgcgggaatg
    16441 cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt gggcagcatt
    16501 aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac aaacagcgtt
    16561 ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac acatttcttc
    16621 ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc acgcgggcgc
    16681 accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat tttgcgacgg
    16741 tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat atgcagagta
    16801 atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc
    16861 gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac aaaacccgcg
    16921 ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg gaaaatcacc
    16981 actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg ttgcgtcggg
    17041 tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg ttggcttatt
    17101 gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca
    17161 ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca
    17221 cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt
    17281 ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac gagataaaga
    17341 acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca
    17401 agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc tgggatcttg
    17461 attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg aagggaaatg
    17521 caacgaggtg tgtaaattgt tggttactgt tacagattga tgaccggcaa aaaaaaacct
    17581 gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact tcacctatca
    17641 atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca
    17701 acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg
    17761 cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg ggcgttttcc
    17821 gtaacactga aagaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg
    17881 atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac
    17941 ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtca cttagctatc
    18001 acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctggtcg
    18061 ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt
    18121 atcacaccga taatttttta ttgattggca acggttacca caacgaacaa aaagagcgtc
    18181 aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga
    18241 tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc
    18301 gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc
    18361 tggcaacacg tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc
    18421 actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg
    18481 gtattgcggc caatgaccgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac
    18541 aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg
    18601 attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg
    18661 agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga
    18721 ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg gctttggcgc
    18781 tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc
    18841 gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc
    18901 agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc
    18961 gcctgaccat tatcaaacag aacttctggt aacagcggac ggctattgta gttggatgac
    19021 attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc cttcacttcc
    19081 ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca
    19141 cataacggtc cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca
    19201 gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg
    19261 aacccggcat caaccagcac aaagtggcgg ctccccattt gtttgacgct ccgcacctga
    19321 gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca
    19381 gggtggccca aatggcgggc gatttgctca cgcgcggcat tccacagacc ataataatgt
    19441 tcggtatcaa ccgcctcttc accctgttga taaggaacag aaagcccacc gcccgcagaa
    19501 atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc acacacctgt
    19561 tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac cagctgcaga
    19621 tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg
    19681 ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg
    19741 cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa
    19801 cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca
    19861 tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca
    19921 cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc
    19981 aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc
    20041 gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca
    20101 gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt
    20161 ggcataacaa actccagata agtgcttttt tatgattacg ccacatcata aaaagaataa
    20221 aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga
    20281 gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc
    20341 ggaagcctga ctgaggcggc acacctgcta cacacctcac agccaaccgt cagccgcgaa
    20401 cttgcgcgct ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg tgggcgatta
    20461 catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg
    20521 gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga actgtctatt
    20581 gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca
    20641 cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg
    20701 ctctcggccc agcgtcatga tttaggactc actgaaacgc tccatacgcc tgcgggaaca
    20761 gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg
    20821 gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat cagcctttcc
    20881 cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga
    20941 cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg ggcgggggta
    21001 ggtgtttcgg tggttaaccc gctcaccgca ctagattatg cggcaagcgg tttagtggtg
    21061 cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct gcaccgcccg
    21121 tcatcagcgc tggttcaggc gtttagtggg catttacaag cggggttacc gaaactggtc
    21181 acttctcttg acgctatttt gtcgtcagct acgatagcat aaaagcgaca gcatcctcgg
    21241 catggatcgc cgcggtatca aacacaggca aaacactgcg ctcttctggc accagtaaac
    21301 caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca
    21361 cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa
    21421 taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt
    21481 gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg
    21541 ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaga
    21601 aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata
    21661 acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc
    21721 cggttttatc ccactcgccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat
    21781 gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca
    21841 gacgatagta aggaatagtg gattcccagc tcattcctcc cagcaaacca attgttttca
    21901 ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct
    21961 caacggccag gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg
    22021 cccgtgaaat cagacgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat
    22081 atgttccagc gtgacatttt tggtttccgg aatgaaccag aaagtgatgc ccacaaacgc
    22141 aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctgt cgagcagcgt
    22201 caggaaggtc gcgccgataa tcatattcga cacccagttt gtcgtcgtcg aacaggtgat
    22261 gccaaaatcg cggcatttca gcggctgaat ttcagagcac aggatccaca ccactggcgc
    22321 ggcgctcatc gcataaccgg caatacacat catcgtcatg ccaacagaga gccatgacag
    22381 gccactggaa gccgtaccgt tatcaaactg catcaggcaa tagcccagca ccagagtgcc
    22441 taacgccatc acgctgaaac caattttcag agccggttta cgccctgctt tatctaccgt
    22501 aaacaccgca ataaaggtgg cgaacataaa ggtcagccct acgaccagag tcgcaatcat
    22561 ctgttgttct gtggtcgtaa agcccgccat tttgaagata cgcggcgcgt agtacatgat
    22621 gatgttcata ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa acacagcacg
    22681 acggacgtta cggttgatct taaacagtgc ccaaccgccc tgttttaact tcaggctttc
    22741 acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac
    22801 ttcttccgcc tcaatatgac gccccttttc cgccagccag cgcgggctat ttggcaggaa
    22861 gaccaccaga ataatcagca ggactgctgg taaagcaaga acccccaaca ttgcgcgcca
    22921 gttaccgcta taactgaacg ctgtatcgga taaaaacgcc agcacgatgc cgagtgtgac
    22981 catcaactgg tacatactga tcatcttacc gcgaacgttt tcacttgcca tttcagaaag
    23041 atacagagga gcggtgtaag acgcgatccc gacagcaatg cccagcacca cccgagcggc
    23101 gattaacatc tctacgctgg tcgcaaaagc ggaccctata gaaccgagta caaacaggat
    23161 ggcccccgcc atcaggctgt atttacgccc caggcggaac gacagccaac cattaaacag
    23221 cgcaccaatt gctgcgccga gcatcatgct actaaccacc cattcctgca aacgactggt
    23281 cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg attacgccga tatcaagacc
    23341 aaataacaat cctgcgaccg cagcagctac cgaaacaaac atattcatac gccgcgtatc
    23401 ccgcaaagaa cgtggcgtta aagcagattc cgtattgata gtaaccattt tttcctgcca
    23461 gcagagaata agacatagtg aaaaaatacg tgaacaattc acacaggtgt caggtcggaa
    23521 atagcataaa tatggattaa attgctacga catgccgtta tgtgatggat attccaattt
    23581 taaaattaag ttgaattatt gagattatta ttaaccacct aattttaaag cagataaaat
    23641 tcataaagtt cattcattga taattgatat ggattatttc ataaccatga tatggattat
    23701 gatgatctac agatataaaa aaccctgcca tgcggcaggg tcataaaagt aagaagaatg
    23761 aattaacgcg ccagccaacc gccatccacg gcaatggtat aaccattcac ataatctgaa
    23821 gcgctggagg caaggaacac taccggcccc atcaggtcac tcggcagtcc ccaacgacca
    23881 gctggaatgc ggtcgagaat ttccgcgcta cgttgttcat ctgcccgcag ttgttgagta
    23941 ttgttggtcg ccatgtaacc cggagctatc gcattaacat taatgttgtg tttagcccat
    24001 tcgttcgcca tcaatcgcgt cacgcccatc acaccgcttt ttgatgcggt ataagaaggc
    24061 acacggatcc cgccctggaa ggagagcatt gacgcgatat tgataatctt gccgccattg
    24121 ccttgcgcga taaagtgttt cgccgctgcc tgagacatga agaatacgct cttgatattc
    24181 aggttcatga catcgtccca gtccttttcg ctgaactcga gagcgtcttc gcggcgaatc
    24241 aatccggcgt tattcaccag gatatcaata tgaccaaact ccgctaccgc gcgatccagc
    24301 agtgctggaa taccatcaat ctttcgcaga tcggcggtca ggcttaaaaa acgacgcccc
    24361 agcgctgtga cctgctcgat ggtttcagtc ggttcaacga tgttaatgcc aacaatgtca
    24421 cagcccgctt gcgccagccc caacgccatc ccctgaccca gtccagtatc acaaccagtg
    24481 acgaccgcaa ctttaccttc gagagaaaat gcacttaaaa tcataacaat accttattcg
    24541 tttatgccca caactagcgc aaatctttaa cggccacatg gtccatatca tcaaagacct
    24601 ggttttcacc gaccatgccc cagataaagg tataagcttt ggttccgaca ccggaatgga
    24661 tcgaccagct cggggagatc accgcctgct cgttatgcat cacaatatga cgcgtttctt
    24721 gcggctgccc catcatgtgg aaaacgcagg cgtcatcatc catattgaaa tagaaataaa
    24781 cttccatccg gcgctcgtgg gtgtgacacg gcatggtgtt ccacaagtta cccggagcca
    24841 gctccgtcag ccccatactc aattggcagg tttccagtac atccgggaca aaatatttgt
    24901 taatcgtgcg acggttactg gtgaggttat cgcctaacgt gactggagat acttcgtccg
    24961 gtgtgacttt tttggtggga tacgtcgtat gcgcgggtgc gcaattgtaa taaaacttcg
    25021 ccggagtgcc ggtatcaata ctggcaaaga caacttcttt tgcaccttta ccaacataca
    25081 gggcgtcgcg gtgaccgatt tcatagcatt ggccatcgac agtaatcgta ccggcaccgc
    25141 caatattgat aacacctaac tcgcgacgtt caaggaaata gcttacgccg agttgtttac
    25201 caacttcccc gccaacggaa accgttttag ttatcggcat aatgccgcca acaataattc
    25261 ggtcaatgtg gctgtaaacc atggtgtact catcggcgac aaataccttt tcaactaaaa
    25321 attcattgcg cagcccttgg gtatccagcg tttttgcgtg cgcactgtgg atgctctgtc
    25381 ttacgtccac atcaacctcc gataatcagt gaagcgtgaa aatagaaaac gatagtgaaa
    25441 cactgtttcg ttttctctat aaagggatga tatgcccatg ggctagggtt ttcaattaaa
    25501 aataaaacaa tgtttcattt ttaagttaag gattaaaaaa agtgcagtgt cgatcacgaa
    25561 tatgtcatga gaatggaaaa atgagggagc ccagaaaatg tatgtcgact tgccagccat
    25621 cattattcaa cagattaatg acggcagata tttatcggta aggaggaaat gcagagctat
    25681 tcgtcacgtt caatggtcaa tgccacaccc tggcccccgc cgatacaaag cgttgccagt
    25741 cctttgcggg cattacgttt caccatttca tgaaccagag aaaccaggat tcggcaaccg
    25801 gaagcgccta tcgggtgacc aagtgcgatc gcaccaccat tgacattgac ccgacgctca
    25861 tcccactcaa gcatcttgcc aaccgaaagc gcctgtgcag caaacgcttc attagcctcg
    25921 ataagatcga catcagccaa ctgccagcct acacgctcca ggcaacggcg ggtcgcatac
    25981 accggcgcaa ttcccatcaa tgccggatct acaccaacgc tggcaaatgc gcgaatgcgg
    26041 gccagcacgg gtaaattcaa cgctcgtgct ttggcttcgc tcatcatcat taccgcagct
    26101 gcgccatcgt ttatggatga tgcattaccc gctgtcacag aaccgagact atcaaatgaa
    26161 ggatttaaac gggctaaacc ttctgcactg gcgtcagtgc gtggctgttc atcggtatca
    26221 acaaccaacg tctgaccgtt actttgggtc attaccggga cgatctcatc tttaaatcgt
    26281 ccggcgtcaa tcgccgctcg cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaat
    26341 tgacggctga tgccatattc gcgagccaga ttttcggcgg tgacaccaat atgataatca
    26401 ttgaaggcat cccataaccc atcatgcaca agactgtcaa ccagctggct attgccaagc
    26461 tgtgcaccgg tgcggctatc agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca
    26521 ccggcgatga caatatcagc ctcgccacac tgtatcgcct gagtagccag atgcagtgct
    26581 ttaagtccgg aaccgcagac gtcattaatc gtgattgcag aaacgctatt aggcagacca
    26641 cctttaatag ccgattgcct tgccggattc tgccctgccc ctgcagtcaa cacctgacca
    26701 agaattactt catccaccgc atatgcagga acgccggtac gttctattaa cgctttcacg
    26761 accagactac caagttccac ggcggaatga cccgctaacg caccgcgaaa gcagccgata
    26821 ggtgtccgta acgccccgac aatcacaacg tctttcatca caacctcgca ccaattaaca
    26881 gtgcaatagt aaatgattgt taattacagt tatcttaatt gtttaaaaaa agtgattttt
    26941 atcacaaagg aaatatgccc gagcagtagt cagagtcata actggcacgt aaggtttgca
    27001 accactaacc caccaataga ggggtagata gggcgttaat ctcccatact taacctggtt
    27061 tatggtaaat tgccctccat tttgtttaat ttgtagatga tacgttcaga taatgtctaa
    27121 tatttggtct aaagaagaaa ctctgtggag tttcgcgctc tacggcacag ccgttggtgc
    27181 aggcacgctc ttccttccta ttcagttagg ttcggcaggg gctgtggtcc tgtttattac
    27241 tgctctggtc gcctggcctt taacatattg gccacataaa gccttatgcc agttcatcct
    27301 ctcatcgaaa acatcagcag gtgaagggat aacgggcgca gtaacacact actatggcaa
    27361 gaagattggt aatctgatta ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat
    27421 atatgcagtg gcaattacca actcacttac ggaacagctg gcaaagcata tggttattga
    27481 tcttcgcatc cgtatgttgg tgagtctggg tgttgtatta atcctgaatc tcatttttct
    27541 gatgggacgc catgcaacca ttcgggtaat gggttttttg gtattcccat tgattgccta
    27601 tttcttattt ctttccattt accttgtcgg tagttggcaa cctgatctat taacgaccca
    27661 ggtagagttc aatcagaata cccttcacca gatatggata tcgattcccg tgatggtttt
    27721 cgcctttagc catacgccca ttatttctac gtttgccata gacagacgtg aaaaatatgg
    27781 cgaacacgct atggataaat gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat
    27841 aagtgtactg ttctttgtct ttagctgcct gctttctatt ccaccttcgt atattgaagc
    27901 ggctaaagaa gaaggggtca ccattttatc ggcgctttct atgctgccga acgccccagc
    27961 atggctgtca atttccggga ttattgtcgc agtagttgcg atgtcgaaat cattcctggg
    28021 tacgtacttt ggcgttattg aaggtgccac ggaggtcgtc aaaacaacac tacagcaggt
    28081 tggtgtaaag aaaagtcgtg catttaaccg cgcactatca attatgttgg tatcgctgat
    28141 caccttcatt gtttgttgca ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg
    28201 cccgctcatt gccatgatac ttttcatcat gcctacgttg tcaacgtatc tgatcccggc
    28261 gcttaaaccc tggcgttcca tcggaaatct gattacgctg atcgtgggta tcctgtgcgt
    28321 atcggtaatg ttctttagct gatatccatt gtgaatggtt caaaattgct acaattttga
    28381 accattccag ttttcat
//
LOCUS       DAEVZA010000053        28389 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEVZA010000053 DAEVZA010000000
VERSION     DAEVZA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.053"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..878
                     /gene="cyoE"
                     /locus_tag="LAF92_002360"
     CDS             <1..878
                     /gene="cyoE"
                     /locus_tag="LAF92_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBI9791359.1"
                     /translation="YLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLVGVSLV
                     VASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLWFGANP
                     LACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDSGAAIL
                     LAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAVATLML
                     SLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALSVMMSV
                     DFMVPDSHTLLAAVW"
     gene            1063..1374
                     /locus_tag="LAF92_002361"
     CDS             1063..1374
                     /locus_tag="LAF92_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBI9791360.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            1358..1900
                     /locus_tag="LAF92_002362"
     CDS             1358..1900
                     /locus_tag="LAF92_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI9791361.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1956..2891)
                     /locus_tag="LAF92_002363"
     CDS             complement(1956..2891)
                     /locus_tag="LAF92_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI9791362.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPENFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            3299..4663
                     /locus_tag="LAF92_002364"
     CDS             3299..4663
                     /locus_tag="LAF92_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9791363.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLTVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(4791..5282)
                     /gene="yajQ"
                     /locus_tag="LAF92_002365"
     CDS             complement(4791..5282)
                     /gene="yajQ"
                     /locus_tag="LAF92_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBI9791364.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            5450..6361
                     /gene="panE"
                     /locus_tag="LAF92_002366"
     CDS             5450..6361
                     /gene="panE"
                     /locus_tag="LAF92_002366"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBI9791365.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVATPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            6324..6914
                     /gene="yajL"
                     /locus_tag="LAF92_002367"
     CDS             6324..6914
                     /gene="yajL"
                     /locus_tag="LAF92_002367"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBI9791366.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6968..8416)
                     /gene="thiI"
                     /locus_tag="LAF92_002368"
     CDS             complement(6968..8416)
                     /gene="thiI"
                     /locus_tag="LAF92_002368"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBI9791367.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            8622..8864
                     /gene="xseB"
                     /locus_tag="LAF92_002369"
     CDS             8622..8864
                     /gene="xseB"
                     /locus_tag="LAF92_002369"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBI9791368.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8864..9763
                     /gene="ispA"
                     /locus_tag="LAF92_002370"
     CDS             8864..9763
                     /gene="ispA"
                     /locus_tag="LAF92_002370"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBI9791369.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9788..11650
                     /gene="dxs"
                     /locus_tag="LAF92_002371"
     CDS             9788..11650
                     /gene="dxs"
                     /locus_tag="LAF92_002371"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBI9791370.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11705..12679
                     /gene="yajO"
                     /locus_tag="LAF92_002372"
     CDS             11705..12679
                     /gene="yajO"
                     /locus_tag="LAF92_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBI9791371.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(12733..13251)
                     /gene="pgpA"
                     /locus_tag="LAF92_002373"
     CDS             complement(12733..13251)
                     /gene="pgpA"
                     /locus_tag="LAF92_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBI9791372.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(13229..14206)
                     /gene="thiL"
                     /locus_tag="LAF92_002374"
     CDS             complement(13229..14206)
                     /gene="thiL"
                     /locus_tag="LAF92_002374"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBI9791373.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(14284..14703)
                     /gene="nusB"
                     /locus_tag="LAF92_002375"
     CDS             complement(14284..14703)
                     /gene="nusB"
                     /locus_tag="LAF92_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBI9791374.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14723..15193)
                     /gene="ribE"
                     /locus_tag="LAF92_002376"
     CDS             complement(14723..15193)
                     /gene="ribE"
                     /locus_tag="LAF92_002376"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBI9791375.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(15282..16385)
                     /gene="ribD"
                     /locus_tag="LAF92_002377"
     CDS             complement(15282..16385)
                     /gene="ribD"
                     /locus_tag="LAF92_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBI9791376.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQDDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(16389..16838)
                     /gene="nrdR"
                     /locus_tag="LAF92_002378"
     CDS             complement(16389..16838)
                     /gene="nrdR"
                     /locus_tag="LAF92_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBI9791377.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16989..17528
                     /locus_tag="LAF92_002379"
     CDS             16989..17528
                     /locus_tag="LAF92_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBI9791378.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            17827..18711
                     /gene="tsx"
                     /locus_tag="LAF92_002380"
     CDS             17827..18711
                     /gene="tsx"
                     /locus_tag="LAF92_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBI9791379.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18888..19235)
                     /locus_tag="LAF92_002381"
     CDS             complement(18888..19235)
                     /locus_tag="LAF92_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBI9791380.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(19364..20335)
                     /gene="secF"
                     /locus_tag="LAF92_002382"
     CDS             complement(19364..20335)
                     /gene="secF"
                     /locus_tag="LAF92_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBI9791381.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(20346..22193)
                     /gene="secD"
                     /locus_tag="LAF92_002383"
     CDS             complement(20346..22193)
                     /gene="secD"
                     /locus_tag="LAF92_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBI9791382.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(22221..22553)
                     /gene="yajC"
                     /locus_tag="LAF92_002384"
     CDS             complement(22221..22553)
                     /gene="yajC"
                     /locus_tag="LAF92_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBI9791383.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(22576..23703)
                     /gene="tgt"
                     /locus_tag="LAF92_002385"
     CDS             complement(22576..23703)
                     /gene="tgt"
                     /locus_tag="LAF92_002385"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBI9791384.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23759..24829)
                     /gene="queA"
                     /locus_tag="LAF92_002386"
     CDS             complement(23759..24829)
                     /gene="queA"
                     /locus_tag="LAF92_002386"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBI9791385.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVGFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24922..25503
                     /gene="acpH"
                     /locus_tag="LAF92_002387"
     CDS             24922..25503
                     /gene="acpH"
                     /locus_tag="LAF92_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBI9791386.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(25508..27325)
                     /gene="malZ"
                     /locus_tag="LAF92_002388"
                     /pseudo
     CDS             complement(25508..27325)
                     /gene="malZ"
                     /locus_tag="LAF92_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(27481..>28389)
                     /locus_tag="LAF92_002389"
     CDS             complement(27481..>28389)
                     /locus_tag="LAF92_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI9791387.1"
                     /translation="KVATIIIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWL
                     GMVMSLQMVMFAYGGIEIIGITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSI
                     YPWNQVGTAGSPFVLTFQHMGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQ
                     GSAPKIFSKTSRRGIPWVTVLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWI
                     MILLSQIAFRRRLPPEEVKALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYV
                     GFAWIVVLLIGWMFKRRHDRQLAENQ"
BASE COUNT         6933 a         7534 c         7114 g         6808 t
ORIGIN      
        1 aatacctgca agtaacgaaa ccaggcatca tctttggcaa cctgatctcg gtgattgggg
       61 gattcctgct ggcctcaaag ggcagcattg attatcccct gtttatctac acgctggttg
      121 gggtgtcact ggttgtggcg tcgggttgtg tgtttaacaa ctacatcgac agggatatcg
      181 acagaaagat ggaaaggacg aagaatcggg tgctggttaa aggcctgatc tctcctgctg
      241 tctcgctggt gtacgccacc ttgctgggta ttgctggctt tatgctgctg tggtttggcg
      301 cgaatccgct ggcctgctgg ctgggggtga tgggctttgt ggtttatgtc ggcgtctata
      361 gcctgtacat gaaacgccac tcagtctacg gcacattgat tggttcgctc tccggcgctg
      421 cgccgccggt gattggctac tgtgcagtaa ccggcgagtt cgatagcggc gcagcgatcc
      481 tgctggctat cttcagcctg tggcagatgc ctcactccta tgccatcgcc attttccgct
      541 ttaaggatta ccaggcggca aacattccgg tattgccagt ggtaaaaggc atttcggtgg
      601 cgaagaatca catcacgctg tatatcatcg cctttgccgt tgccacgctg atgctctctc
      661 ttggcggtta cgctggatat aaatatctgg tggtcgccgc ggcggttagc gtctggtggt
      721 taggtatggc tctgcgcggt tataaagttg ctgatgacag aatctgggcg cgcaagctgt
      781 tcggcttctc tatcatcgcc atcactgccc tctcggtgat gatgtccgtt gattttatgg
      841 taccggactc gcatacgctg ctggctgctg tgtggtaaca aaacctctct attaaaaagg
      901 tgctacggca ccttttttat tgtctgagat cagagatacg gctaataacc gtatgtcttg
      961 acagagtgag cagatgaaca taaattaatc atacggttga tagccgtatt atgtatgaga
     1021 tcgactccct acgatttcat tcagttaatc aaagaggtca taatgcctgg aaaaacagcc
     1081 actctacccg atgtcgataa aacgctgaaa aatgcgcggg tcgaactcaa aactagtccc
     1141 gacgccaaaa acaaacttcg tgaagctgcg caagccgttg gggtagattt aagcgctttt
     1201 attttgagcg ctgctatgga acgcgccgaa agtgtgcttg ataaccaacg tcgccgtgag
     1261 ctttcgaatc aaagctggga actgatgaac caactcatcg ctgaacctgc tcaaccgacg
     1321 ctcgccctca aggcgttaat gaaaaggaaa aacagcgatg gtcgacaagc atgaagagat
     1381 tactctgccc atagtcctct cctgtaatta tcagtctgat attacttatc ctgggcaaaa
     1441 acagtttgat tgcggtaacc ctgttatcga taaatttgta cgcgcatcgc taaagaaaag
     1501 tgtgcgtaat agcgactgtg cggctaaagc acttattgac agacaaagtg gtgaactgat
     1561 cggcatctgt acttttacgg catattcgct ggaaaaacaa cgcgtttctg gcgtccttca
     1621 gggttcacaa ccttcagaaa ttggtgttgt cagattagtc atgttggggg tagcacggaa
     1681 gtatcaaaag cggggctttg gtcaggacct actatgtgat ttttttgaac atgtaaaaat
     1741 aattcaccag gcattaccaa ttaaaggggt ttatcttgat gctgaccctg ccgccattaa
     1801 tttttatgct cgtctcggct ttgttcagct ttcagcgaca ccaaatgctt ttggtgctgt
     1861 acctatgttt ttggcgattc agcatattct cgcggcttag attattatcg ccacccgcca
     1921 gggcaagttt tgcctcagcc gggggctgag cagcgttacc acttttgttc aattgccccc
     1981 gctttcagca actccacatc cttgccgtta atggttgccg ttgcggcaac gattgtggtc
     2041 cttacagttg cacaagattt tcgctcaaat tccggtttat aaagaataag agacgaatta
     2101 ctggtaaatg aaccaaatag gtaatcagta aagctaatat tcagccggtt taaaccgaaa
     2161 ggcgcgaagc caaccgtttg gttaatctcc ttaccggatg ggcgacgaat gctgaatgtc
     2221 acgcgaagat tggccacttc gcctaattta ttggtggtgg cgaagaggct atccatgcag
     2281 gaaaaacctt tatctaaaaa attatctcgc atcccagaac gggtaaccgt aatcacgggt
     2341 aacgtatcgt caaccgtttt tctgtcaatc atgtcttttc gacaagtagc cagaaagttt
     2401 tctgggccac cataaatgtg gacaccttta tcaagatatt ccttcgcttt cttacaatct
     2461 acctcaacat actgaccatt ggcgtacata atgcccaacc tggaccatgc taaattcaca
     2521 ccgtgctgcg cggcctgatt aaatagtgca tacgctttgg cataatccac cctcacctgc
     2581 tcaccgtcgt agtagatacc ccccagcgct aacggagctt ctctgtcacc ttgtttagcc
     2641 gctaactcga accatttaat cgcttcctga acatcaggtt tggtatcata acgaggctga
     2701 taatattccc agcctaacag gctctgcgcg tcgatatctc ccgattcagc cattttatgc
     2761 agtgtattta taacatcggg agcaggttta ccaccgtata tgctgtcata caattcatgc
     2821 gctttctcgc tggccaccac gcttggtgaa actatcagca ttacagaaaa taatattctt
     2881 ttagttaaca tgtatacctt tatgtcctca ctgtcatata tttccataat aattaaccac
     2941 caaacatcca tttaatgaat aaatacagtg ccataaaggt ccaagtaata agcacacatt
     3001 tagcctgaaa cattatcatc catgtcacca aatcgtttat ttagtgagat aaaactagca
     3061 tattcgtaag ataaaaaatg cacatagcaa tcaagaaatg atattattac ttttttgatg
     3121 taaaactatt tagttttaaa attcattttt tcaacatata gagcattcat tttatgaatg
     3181 caaaaaaaca tacctgacac acaaattact ctcctctgta atattttctc attaacactc
     3241 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
     3301 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
     3361 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
     3421 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
     3481 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
     3541 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
     3601 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
     3661 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
     3721 cgtgagcttt ggcattacct ttgctattgc gatggtgctt ggcccgatca tcactcacaa
     3781 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacgaccg gcattgcgtt
     3841 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
     3901 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
     3961 tatgtgtctg catattttgc tgatgtcgac gtttgttgcc ctgcccggac aactggctga
     4021 tgcagggttc ccggcggctg aacactggaa ggtctatctg gcgacaatgc taatcgcctt
     4081 tggctcggtc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
     4141 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cgcaaacgca
     4201 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     4261 cctcctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     4321 tgtttactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     4381 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     4441 gctgacagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     4501 cccggcgaac attgccgcaa acgaggcgtt aaaagtgcgt ttgctagaaa ctgaaggcat
     4561 caaagaagtg ttgattgcag aagaagaaca ttcagcttat gtgaaaatcg acagcaaagt
     4621 gacgaatcgc tttgagatag aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     4681 ctgctgcgct acgcttatca ggcctacaaa gtttctgaat tacaaacctt tgtaggccga
     4741 atcaggcgtt catgccacat ctgacatgaa caaaacgcac atagtcgcga ttaatcgcgg
     4801 aagtttttga actggaacgg ctgaccgaga tcgccaccac gtaccatcgc catgacagcc
     4861 tgcaaatcat cacgagattt gcccgttacg cggatttcat cgccctgaat ttgcgcctgc
     4921 actttcagtt tgctgtcttt gatcatcttg acgattttct tctgggtcgc gctctcaatg
     4981 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     5041 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     5101 aactgattga cctggaagtc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     5161 agctcaaatg aggcttcaac gttacggaag tcaaaacggg actccacttc gcgactcgcg
     5221 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     5281 atcttttcct ctcccttcat ttttgatgcg aagcataata cctgcaaagt taaataacca
     5341 acagtcctgc accaggacgt atcccttaat tgtgcatagt cgcaacagca tgcacaatta
     5401 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     5461 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     5521 tgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     5581 agatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc tcgccaccag
     5641 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     5701 atccacactg cctgtagcta cgccaatact gttaattcac aacggcatgg gcaccatcga
     5761 agaattgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     5821 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catatcggtc cggcacggca
     5881 acaggatggc gattacagtt atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     5941 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     6001 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     6061 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     6121 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     6181 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     6241 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     6301 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     6361 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     6421 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     6481 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     6541 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     6601 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     6661 tggtgccgca cgatatcttc ccgattggta atatgaccgg cttcccgacg ctgaaagaca
     6721 aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     6781 tgaccagcca ggggccgggg acagctatcg actttggtct gaaaattatc gacctgctgg
     6841 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     6901 attattacga gtagtgtcgg atgcggtaag cgtcgcatct gaccagatgc gacgttacaa
     6961 ccacaaatta cgggcgatat accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     7021 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     7081 gatcgccaaa cttggtgctc agtttataga acggcagaga aactacatca atcccttcga
     7141 ctttcagtgg cttatcttcc tgttcatcga tagaacggat atcgaggatc acgtcgttcg
     7201 ggccgaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     7261 cgcggatatc aacgttattc gcttcttcaa ccactttatc gagaatgctg aagtcgaatt
     7321 tctcttcttc cgcttcaatc ttcgacttaa ccgctttcac cgtcgggctt ttggagataa
     7381 caccgcaata ttccggcatc gtgcgagcaa agtcttcggt gccaatctgg cgagccaggt
     7441 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcggagacgt
     7501 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     7561 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     7621 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     7681 gttcgaaatt aatagcgaca aaacgcacgc ggtgggagct gccaaaacgg ttccacagat
     7741 aatgcgccac ctgacgcacg ccaatttcat gcgccgcgcc gccaaggtta aagaaacagt
     7801 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     7861 cggaaatgag cgacagcaca tcttcctggg tgccgatcgg gaaaccgcca ataccttcgt
     7921 agcggccttt aatcagcagg agacgatcgt cttccacttc cagatggaca gtcacttccg
     7981 gattggtcag cttcacgcgc gcggactcaa tatgctgatt taaaccgccg ccgacgtaac
     8041 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     8101 ttttgccttc cagctgatcg cgatactgaa ccaacgcttt ctcaaaaata tcgtgcatgt
     8161 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     8221 cgtcgcgaat agccagacgc tggttttcat cttttgcgcg aacttcgatg ttatcccagt
     8281 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     8341 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     8401 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     8461 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     8521 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     8581 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     8641 ctcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     8701 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     8761 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     8821 atgaagacgc ctctctaacc ccttttacac cggacaatga gtaatggact ttccgcagca
     8881 actcgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     8941 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     9001 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     9061 ggacgcaccc gctgccgccg tagagtgtat ccacgcttac tcattaattc atgatgattt
     9121 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     9181 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattctaag
     9241 cgatgctgat atgccggaag tgtcggatcg cgacagaatt tcgatgattt ctgaactggc
     9301 gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag acgcggaagg
     9361 caaacacgta cctctggacg cgcttgagcg tattcatcgt cataaaaccg gcgcattgat
     9421 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     9481 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     9541 ggatgtggta ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     9601 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     9661 gatcgacgat gcccgtcagt cgctgaaaca actggctgaa cagtcactcg atacctcggc
     9721 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     9781 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     9841 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     9901 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     9961 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
    10021 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
    10081 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
    10141 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttgctgccga
    10201 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
    10261 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
    10321 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
    10381 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
    10441 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
    10501 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
    10561 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
    10621 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
    10681 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
    10741 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
    10801 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
    10861 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
    10921 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
    10981 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
    11041 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
    11101 ttacctgcgc tgtataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
    11161 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
    11221 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
    11281 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
    11341 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
    11401 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
    11461 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgttaatgg cccatcgtaa
    11521 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
    11581 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
    11641 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
    11701 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
    11761 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
    11821 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
    11881 tagctattcc gatggtagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
    11941 tcgtgaagac gtggtcgttg ccaccaaagt gttccatcgc gttggtgatt taccggaagg
    12001 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgacgtc tcggcatgga
    12061 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
    12121 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
    12181 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
    12241 tgtcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
    12301 actgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
    12361 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
    12421 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacag gcgtcagcga
    12481 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttgttgagta aaccgggcat
    12541 tgccgcaccg attatcggaa cttcgcgcga agaacagctt gatgagctat tgaacgcggt
    12601 ggatatcact ttgaagccgg aacagattgc cgaactggaa acgccgtata aaccgcatcc
    12661 ggtcgtagga tttaaataag agtccaggcc tgatgagacg caacaagcgt cacatcaggc
    12721 actggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
    12781 acctgcggag atcaccccgg cgacaatatc atcgatcatg atccccatgc cgccatgtac
    12841 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatgac
    12901 gaatccggca gtgacccact gccagtcatt ggtcggcaat gccatgagcg tgatccacat
    12961 gccgataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
    13021 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
    13081 ccagggtaaa aaggtcatca gataccagaa agggatcgcc gccagcgatc ccatcgtgcc
    13141 aggaacgatc gggcttaatc cactgccgaa tccgacagca agcagatgcc acggattact
    13201 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    13261 agtcaaaggt gacaggttcg ccgtcacgga taaaacaaag cccttcgata tcggcggtca
    13321 tctgcccgat acaggtaaac ggtacgccca ggtgtccgag agccacatcc agcgcgccac
    13381 ggttcagttc cggcacagtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    13441 gcagcgcctg ttcaggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    13501 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    13561 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgtccc tgtaaaatac
    13621 gcggcgatgg acggagatga cgtttgatca agtagtccgc atctttagca tcggcaacct
    13681 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    13741 agatccagtc acccggtttc gctccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    13801 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    13861 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    13921 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    13981 gatcgcttag gttcaccgcc agtgctttat acgccagatc agcaggatcg atatcatgga
    14041 ggaagtggtt gcccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    14101 tattgagaag agcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    14161 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    14221 caggtaaaaa agaaaaggcc ggcgatcgcg gaacggtctt ccgtgaatct accggcctgg
    14281 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    14341 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    14401 tttgtatggc acatcgctac gtttagacag ctcgtacagc gcaatgcgca gtactgcttt
    14461 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    14521 gtatgcggtg ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    14581 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    14641 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    14701 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    14761 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    14821 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    14881 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    14941 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    15001 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    15061 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    15121 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    15181 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    15241 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    15301 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    15361 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    15421 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    15481 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    15541 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    15601 tcactagaat catcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    15661 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    15721 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    15781 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    15841 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    15901 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    15961 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    16021 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    16081 tcttgcatcg aggcaaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    16141 ggcgtacgac catgatggct acagggttcg agtgtgacat aggcggtcgc acctttggct
    16201 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc acgttggtgg
    16261 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttggga
    16321 tgcgtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    16381 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    16441 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    16501 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    16561 gatttaatat gattgattgc catttcgacg tcatcggaac tcaccggacg tttttccagc
    16621 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    16681 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    16741 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    16801 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    16861 aacagaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    16921 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    16981 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    17041 gcgcctgtgt gcagcagagc gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    17101 aggagatgac gcaactcaat aaagaaacgg tcaaaatcac tcagcaaaac aggctgaatg
    17161 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    17221 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgatgca gatggcacca
    17281 ccctgacgct acgtattcag ggcgagtcga atgatccctt gcccgcattc agcggaactg
    17341 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    17401 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacagt
    17461 taaagggtat ttctgtagaa cagttagact tcgtacgtat ccacgatatt caacctgtta
    17521 tgcagtaaac gtatttcggg gcgattttgt gcgtcccgca acatctttcc ccgtcatttt
    17581 gttactccgc ttacatcacc cagattgata gtaaaagttt gcaacaaggg tgaaagtcag
    17641 tacaatccct gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    17701 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    17761 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    17821 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    17881 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    17941 gttaacgttg tcggaagcta tcacacccgt tttggaccgc agatccgcaa cgatacctac
    18001 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    18061 ccggtattct tcggcggtaa ctccgatgcc aaaggtatct ggaaccacgg ctctccgctg
    18121 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    18181 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    18241 gatggtcgcc agagcacctg gtatatgggt ctgggtaccg acatcgatac tggtctgccg
    18301 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatgg cgcagcgaac
    18361 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    18421 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    18481 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    18541 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    18601 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    18661 gttcgctcta ccggctgggg tggttacctg gttgtaggtt acaacttctg attatgaaaa
    18721 tgccgggatt tattcccggc atttcagatt gttaacaacg tgcgctttgt tcatgccgga
    18781 tgcggcgtaa acgctttgtc cgacccacaa aagcacgtaa attcaatata ttgctgaaat
    18841 aacgtagtcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    18901 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    18961 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    19021 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    19081 gtatggtcgt gatcaatgtg gtgaacagta agttcacgca ggttggaata gacaaactcg
    19141 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    19201 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    19261 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    19321 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattaaggc agaattgacg
    19381 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    19441 gtttcagagc caacgcagat gccacataga tggaagatgc agtaccgatg gaaacaccga
    19501 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagaggtac agcatcagga
    19561 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    19621 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaatacggt
    19681 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    19741 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    19801 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    19861 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    19921 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    19981 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    20041 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    20101 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    20161 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    20221 cgatagcggc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    20281 agtcatatac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    20341 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    20401 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    20461 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    20521 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    20581 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    20641 accgccaccg caagggttaa gacgataccc gcgatacctg gcatactcag cgttgcgcct
    20701 ggcaacagcg acataatgcc gacgattaag atcaagttgg caatcagagc actggtcgca
    20761 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    20821 aggcaagcct ccagcccctg ttcaatgttc tgcataccca gggttgggcc aatggtacgt
    20881 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    20941 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    21001 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    21061 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    21121 ttagtgaagt tagacatgat gttaccacca gcgctatcga gcgagatgtt aacctgcggt
    21181 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    21241 cgtttgtaca gcacaactgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    21301 acgcgaccgg atgccgcagc ggcctggtca acgttggtgt ttaccagacg gaattccagc
    21361 gttgccgtcg cacccagaat ctctttcgca cgcgcagtgt cctgaatacc tggcagttca
    21421 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    21481 acacggttac gcaggatatt gatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    21541 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctactaat caccaggtcc
    21601 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagta
    21661 atgctcaggc cgtagttgtt ttctttacga acggtggtat acgggatgcc cttttcacgc
    21721 agatcgctgc gcaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    21781 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    21841 gcgtgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    21901 ttgtcaccca taacgcccat taatgcttca cgagcgcgca gctgggtgtc agtggagtcg
    21961 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    22021 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    22081 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    22141 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    22201 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    22261 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    22321 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    22381 ttggcaatgg agtccatcag ctttttgtgc tctttggtgc gcttctgctg tggacgcagg
    22441 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    22501 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    22561 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    22621 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    22681 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    22741 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    22801 ctcaggatcg agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    22861 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    22921 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    22981 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    23041 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    23101 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    23161 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    23221 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    23281 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    23341 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    23401 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    23461 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    23521 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    23581 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    23641 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    23701 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccgcggaatt
    23761 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    23821 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    23881 aaccagcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    23941 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    24001 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    24061 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    24121 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    24181 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    24241 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    24301 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    24361 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    24421 ccgagcgttc atcattaaat ccgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    24481 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    24541 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    24601 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    24661 ccagaagatc gccggggttg agcttatcga gtaaatcggt gaaagtaccg tgcgtcagcg
    24721 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    24781 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    24841 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    24901 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    24961 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    25021 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgatgt attaaccgac
    25081 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    25141 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    25201 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    25261 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    25321 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    25381 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    25441 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    25501 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    25561 tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac ggtggcgcgg
    25621 gtagcaccac ttcacaggcc tcgccacggt tgattgccac cagtacacgt tgctgattca
    25681 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    25741 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    25801 tatcctgctt ttccacctgc caagggaacg gtttacggca aaacggatcg tttttgccat
    25861 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    25921 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    25981 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    26041 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    26101 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    26161 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    26221 cttccggctg ggtttctttc gccgcttcgg tgatcccagc aacgtgctgc atattattgc
    26281 gtgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg tccatactcc
    26341 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    26401 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    26461 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    26521 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    26581 cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa cgcagcaacg
    26641 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    26701 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    26761 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    26821 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    26881 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    26941 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    27001 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    27061 gatcgtgcca cagcagtttg aaactctaac gccgccgggg ttgtccgctg gagagatcaa
    27121 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgctgctta tgcatcgata
    27181 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    27241 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    27301 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    27361 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    27421 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagta
    27481 ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc caatcaacag
    27541 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    27601 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    27661 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga aggcaatttg
    27721 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    27781 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    27841 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    27901 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata catcactgtt
    27961 aattgccgac agcgaagcag tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    28021 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    28081 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    28141 ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    28201 gataatttcg atcccaccat aagcaaacat caccatttgc aacgacatca ccatgccaag
    28261 ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg tcggttgccc
    28321 gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga tgatgatggt
    28381 ggcgacttt
//
LOCUS       DAEVZA010000054        28245 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEVZA010000054 DAEVZA010000000
VERSION     DAEVZA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.054"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1449)
                     /locus_tag="LAF92_002390"
     CDS             complement(<1..1449)
                     /locus_tag="LAF92_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBI9791388.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEES"
     gene            complement(1531..5004)
                     /gene="bcsC"
                     /locus_tag="LAF92_002391"
     CDS             complement(1531..5004)
                     /gene="bcsC"
                     /locus_tag="LAF92_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HBI9791389.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(4986..6092)
                     /gene="bcsZ"
                     /locus_tag="LAF92_002392"
     CDS             complement(4986..6092)
                     /gene="bcsZ"
                     /locus_tag="LAF92_002392"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBI9791390.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(6099..8438)
                     /gene="bcsB"
                     /locus_tag="LAF92_002393"
     CDS             complement(6099..8438)
                     /gene="bcsB"
                     /locus_tag="LAF92_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBI9791391.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(8449..11067)
                     /gene="bcsA"
                     /locus_tag="LAF92_002394"
     CDS             complement(8449..11067)
                     /gene="bcsA"
                     /locus_tag="LAF92_002394"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBI9791392.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(11064..11816)
                     /gene="bcsQ"
                     /locus_tag="LAF92_002395"
     CDS             complement(11064..11816)
                     /gene="bcsQ"
                     /locus_tag="LAF92_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBI9791393.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTQL
                     SDICSSLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(11831..12016)
                     /locus_tag="LAF92_002396"
     CDS             complement(11831..12016)
                     /locus_tag="LAF92_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBI9791394.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQ"
     gene            12288..13859
                     /gene="bcsE"
                     /locus_tag="LAF92_002397"
     CDS             12288..13859
                     /gene="bcsE"
                     /locus_tag="LAF92_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBI9791395.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAATAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            13856..14047
                     /gene="bcsF"
                     /locus_tag="LAF92_002398"
     CDS             13856..14047
                     /gene="bcsF"
                     /locus_tag="LAF92_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBI9791396.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            14044..15722
                     /gene="bcsG"
                     /locus_tag="LAF92_002399"
                     /pseudo
     CDS             14044..15722
                     /gene="bcsG"
                     /locus_tag="LAF92_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
     gene            complement(15809..15916)
                     /locus_tag="LAF92_002400"
     CDS             complement(15809..15916)
                     /locus_tag="LAF92_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI9791397.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16292..16399)
                     /locus_tag="LAF92_002401"
     CDS             complement(16292..16399)
                     /locus_tag="LAF92_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI9791398.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16775..16882)
                     /locus_tag="LAF92_002402"
     CDS             complement(16775..16882)
                     /locus_tag="LAF92_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI9791399.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17358..18629
                     /locus_tag="LAF92_002403"
     CDS             17358..18629
                     /locus_tag="LAF92_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBI9791400.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(18659..19663)
                     /gene="dppF"
                     /locus_tag="LAF92_002404"
     CDS             complement(18659..19663)
                     /gene="dppF"
                     /locus_tag="LAF92_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBI9791401.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19660..20643)
                     /gene="dppD"
                     /locus_tag="LAF92_002405"
     CDS             complement(19660..20643)
                     /gene="dppD"
                     /locus_tag="LAF92_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBI9791402.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(20654..21556)
                     /gene="dppC"
                     /locus_tag="LAF92_002406"
     CDS             complement(20654..21556)
                     /gene="dppC"
                     /locus_tag="LAF92_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBI9791403.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(21566..22585)
                     /gene="dppB"
                     /locus_tag="LAF92_002407"
     CDS             complement(21566..22585)
                     /gene="dppB"
                     /locus_tag="LAF92_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBI9791404.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(22736..24343)
                     /gene="dppA"
                     /locus_tag="LAF92_002408"
     CDS             complement(22736..24343)
                     /gene="dppA"
                     /locus_tag="LAF92_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBI9791405.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(25088..25221)
                     /locus_tag="LAF92_002409"
     ncRNA           complement(25088..25221)
                     /ncRNA_class="other"
                     /locus_tag="LAF92_002409"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(25256..25332)
                     /locus_tag="LAF92_002410"
     tRNA            complement(25256..25332)
                     /locus_tag="LAF92_002410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25296..25298),aa:Pro,seq:cgg)
     gene            complement(25424..27115)
                     /gene="eptB"
                     /locus_tag="LAF92_002411"
     CDS             complement(25424..27115)
                     /gene="eptB"
                     /locus_tag="LAF92_002411"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBI9791406.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            complement(27439..>28245)
                     /locus_tag="LAF92_002412"
     CDS             complement(27439..>28245)
                     /locus_tag="LAF92_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9791407.1"
                     /translation="LISAFAIGSYGLGSLGFKFIDTQLLETVGLEKTFVIWGAIALVM
                     IVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWMLAVMFLTACMSGLYVIGV
                     AKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDKIARIRVITIGQVISLVGM
                     AALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIG
                     SIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQKMLREAHGSL"
BASE COUNT         6580 a         7790 c         7193 g         6682 t
ORIGIN      
        1 ggactcttcc agcacccagt gaccgacggt aaccatcagc ccacagcact caatgcgatc
       61 gattaagcca tccggcaggt cccaactgcc atccggttgc tggatacgca gtaacacttc
      121 cgcactgacc agtttaccgc tggtcatctc gacctgtggc tgtaaccaaa ttgcaaactg
      181 atgattttcc agtgcattaa ggatatcgct ctcttccgtc aaccgcttct gggcggcttc
      241 catctgctgc ggatcaaaga actgaatctg attcttgcct ttatggcgag cggtaaatgc
      301 cgcagaaata gcgcgactgt aaagctgttc ggcggtgaga tcgccgtaga acatcgccac
      361 gccaatgcta cagtgcggac ggagttgaat acgttcaatc ggcaggcgct cgctcatgat
      421 agtgagcact tgctgaccta aggtgattgc gtgccacggt tcctgtacac cgttggcaat
      481 gacagcaaag tcataaccgc taatctgcgc gaggatcata cgtggcgaca gtaccgattt
      541 gagtttttcc accagcgtca gcagcagaat ttctcgttgc gcctctttca gcacgcccgc
      601 agtatcacgc agggtttcac aggtgatgat catcagcgcg gtggtttgtt tacgcgcgac
      661 aacctgctcc agcatctcca tcagcaaggc tttgttcggc aaatccgaca ccgggaagcg
      721 catcgcattt tcgttctgtt cttcataatg gcgctgcagc aattgctggt tgaggttgta
      781 actgcgcacc aacataccga tttcatcgtc ctgatgcaga cgcggtaatg ccagttggtg
      841 accaacaagc tcctgggctg ggatggcgtt aagttcgcga gcaatattgc gtaacggatg
      901 caaaatcagg cggttaatgc accagctgat ggcgacggtc aggataagcg acaaaagtaa
      961 gtaaatggtc actaacgttg agagggtgct catcacgaac ttatacatac ggaaggaatc
     1021 cgcctgtagt accagatagg caattggctg cgggtttgcc ggacgttcga gcgagtaaac
     1081 gcccagcgag atttgaaccg gtagctcaaa caggcgagta accattaccg gcaccgggcg
     1141 ctctggaata aaacttttac gcagcgcctg gaactggtta ggcagcacta catcggcacg
     1201 gctgaccacg cctgccggtt taatgctggc aagaatagct tccgcttcgg gaatatcgcc
     1261 tttcaaaata gctgaagata agggttcgcg gacagagcga gcaatgcttt ccagttgcgt
     1321 agccgtgtta tagcgattct gttggaccag atggaacagc aaaacggtgc aaaaaataaa
     1381 aacgaacacc aggacaacgg ctgccaccat tgccatctgc ttgattgtta acgagcgact
     1441 tacgcgcaaa ttgtctcccc ccggaaatcc cagattgccg cccgagtata cccgatcgca
     1501 gcggcattaa gagaggcgct atctgaaaac ttaccagtcg gcgtaaggta tcagcggctg
     1561 cggcggtaaa tccatgtcac cctgccatcc ggcggcggaa taacgtacgt agagcaggaa
     1621 atggctgggt gcgtaatctt tcgcctgctg gatatcaatt gccgtgccaa caaaccagtt
     1681 ggaagtaaca cgtcgttcaa gtaatgcccg cgccgtgtag ccgaagccct gactgctgcc
     1741 gccatcgttg gattgccgcg cagcttcttc ctgccagtcg gtcgggatca gattcatcag
     1801 cggataacgc ggcatggttt tggtgcgtga atgcgaccac gagccagacg cccccagctc
     1861 ccacgaccag ttttccgtgc gctcccgcca catcaccggt atggcaaacg acaggtattc
     1921 ctgcggactg tagtaaccgc cctgaccgag tgagtagcca ctcagatctt tgtcgtaatg
     1981 ccagatcatg ttgttcaggc cgattgtgac gcggcgattg ttctggttaa tgaccttata
     2041 gtaatagccc gtcatccagc gcacgcgcca gttatcttcg acatttttac cggttaactg
     2101 gtcgccacta agcgatgccc agacgccgtt tgcttcacct ttatcgtagc tcagacttag
     2161 ccccacaccg tcggcacgta cgccacccca ttttttcccg gtattgctcg gggagtcttt
     2221 ttgcccacca aaggccagca aagaactgga gatgggccga cggtgggcgt taacggtgta
     2281 acccagcggc ccgatatcat cgctgtaact gatgccgccg accacatcca ccacgttgaa
     2341 gcccatcggc gtggtaccga tatcccagct ccagacgtca tttcgccagc cgaccgccac
     2401 gctggcaccg gaatccgact ggctgcggtt gccgctacag tcctgtaatg tacaggtgcc
     2461 ccagttgtca tcccatttgc catcggcatt agtggagaaa ctgccgacgt tcatattgac
     2521 gaaatcactg cgaaagaaca tccgcccgtc agaatacggc gcatccacct gcaacatggt
     2581 agtgtgcgct ttcagatcgg agtaaccacc ggtgccgctc gaaccccagt aatcgtgctc
     2641 aagggtgacg ttaagatcct gctggcgata gaggtccgcc gcatcgctgc gcacgccgcg
     2701 tttcagccag tcatctttct cgtcgttacg ggtcagtcgg gtgaatgtgt cgttatcctg
     2761 cggacgcgtc gtagtcacac cggatgcgac catggcgtct ttgtaggttt ccagcgcctg
     2821 ctttggatcg cccgcctgcg cttcaaactt cgcaccgtcg cgcagcacca tcgcgctttc
     2881 catcgacggt ggctgagatt ttgcctgcgg gatcaactta ttaaacgtct gctgcgccgc
     2941 tgcggtatct ccaagctgcg cctgcgccag cgccacgcgc cgctgtgtgt tcagcgaggc
     3001 gttatcggta gcgggcagtt tcgccagctg gctacgtgcc gccgctgtgt caccggcagc
     3061 aatatccact tccgtcagcc ccagaatggc atcggcgtta gttggctccc gcgtcaggac
     3121 attctgatat gcagcgcggg cggcggtgta atcacgtcgc tgctgcgccc agtcagccag
     3181 cgtgaggtca atacgcgtgg aaggtggttg ctgacgcagc atcgcttccg cttcagcttc
     3241 tttgccgctt tctcgcaggc gattagcggt ttccagcacc tgatcgcttt gcagtcgatt
     3301 aaccagctcc tgaatattgc tgttccactg cgcgcgcggc aggctgttga tatgcgccag
     3361 cgccgctctg tcctggtcat gaccagagag gtacagcccg taagcgtaaa cctgctccgg
     3421 gtcgttcggc ttctgctgcg ccagattgcg cattaacgta tcggcctggc tgcgttgtcc
     3481 ggcctgccag agatcctgcg aaagtcggta agtaatccat acgctgccgg ggtccagcgc
     3541 cagtcgttgc cgctgaagtg ctgccgcctg cgcccatttg ccctggtttt ccagtgcctc
     3601 tgcctgctgt gccagacggt cgttttgcag gctgcgttcg atatcatcaa tgctacgccg
     3661 ctgactggca gagagcgagg cgataaacgc ttcagctttt tctggcgatt gctggcggta
     3721 aatatttgcc agcccgcgca cggcgttagt gttgccgctg tccatacgca aggtctgctg
     3781 ataataacgt tcggcggcgg gataatcttt tcgcgccatc gccacatcgc ccagccccag
     3841 cactgcataa ctgtcggtgt tatcgacatt acgcgcctgc tggaacaggc gttctgcccg
     3901 gtcaggatta ttggctttca gcgcagcatc gccctgctgg atcgccagcc agtagcggtt
     3961 tactttcagc agactgttcc atttgtcgtt gttgctgctg tgcgggtcca gtgcgagggc
     4021 tttttccaga ttcgccactg cattggcgcg atcgcctttc tgagaatacg cctggcccag
     4081 cgcccccaga gcttcactgt ctttcgggtt cgcccgcacc gcctgttgta gttcgggaat
     4141 ggctttaccc gccataccag agtccaccgc cgctaaacct tgcgcacgag cgcggaaagc
     4201 aggatcggcc agctgttttt gctgttctgc cagttgcgat tgcgcagccg ccacgctatc
     4261 gccatcacta aagatcgaga gatatttttt cagcgccgac acactggcat cactgacggg
     4321 catgtcttta atctgcccgt accagatttt agaggcccct tcgcgcccgg cgttcgattt
     4381 tgccatctgt tccaggacgg caaaaccttc gtcacggcga tcgctactaa acagcaatag
     4441 cgccagattg ttttgcaggc ccgtattgcc cggtgcatcc gcattgatgc gttttaactg
     4501 attaatcgct tcgccacggc gagccggaat tttcgccacc gtactccagt actcgacagc
     4561 aatgtcacct tccggcggcg caccgttgaa cagtttgttg taactcgcca cagcttcttc
     4621 tgcatgaccg gtcgtcgcct gcaatcgtgc ctgttgcagt gcctgacgac catccggcgt
     4681 ggaaagtagc atcgtagtcc gcgacgattt atacgcattt gaactcggcg ctaactgcga
     4741 cagccgatcg agctgttttt gcgcgccatc aatatcgccc tgacgtaaca aagaacggaa
     4801 acgggcggca acgacgtccg ggttattcgg atcaataagt tccagccgat ataacgactg
     4861 ttgcaccaga tcttcacgat gggtcgcttc gcctaaccga acttgctcca gcaactgttg
     4921 ctgagcggtt ggtgctgcct cgaccatcgg catgacggcc agaccgaggg aaagcgtgaa
     4981 tatgtttagt gtgaatttgc gcattcctgg ccccagtcag gtaataactc accttttgtc
     5041 gagaagcgga aacggtgttg atcccagcct tgtccaaaca gggtcagcac atagttgtaa
     5101 taggcatcgc tgccgggaaa gttatcggcc acgcgctggc gctgaacggc ctgcgcatcg
     5161 cggttttgta aaaagggcag catggcggca gaaaaaccga ccggtccttt accctgcgct
     5221 ttccccgtag ccacatccac tttttccggc ggataaccgt ttttctcagt gaatgtcgcc
     5281 atcggtttaa accggttgag catccgcgct ttttgcggat cgctgtcagg catcatgcct
     5341 acccacatgt aaacgcggat agcgtcgtag ctgctgatca atgttttttc ggcttttagc
     5401 tgccagcctt tgtctttctc atagcgcacc cagtctggcg aaaaaccttt cggggcggtt
     5461 tccagcaata aacgttgatt ggtttcgcgc agcgtggtcc acggcgcgcc aaagcgggtg
     5521 aaatactgcg ccagcgtcgg cggcaggtag ctggggttaa aacgccagct gttatcctca
     5581 gcaaaaccca ctttgcccgg taacaacatg gaacccagcc caggcaccgt caccacttcc
     5641 tcccgcgcga tacgttttag caacgcgctg ccgatgtcgg tataacgctg ctctttccac
     5701 aaacgccccg cctccagcaa cgaccaggcc atccagacat caccatcgga ggccgaattg
     5761 ctgtccagca cttcccactt actgttctct ttcttgcccc acagccaggc gggcaaacgt
     5821 tcttttaaag aaccctgagc gagattgttc tgcgtccagt cgagaatatt atcgaaagct
     5881 acacggtcgt tagccgccag ggcaaagaac ataccgtaac tttgcccttc ggaggtggtg
     5941 attttgcgcg cgtcgctggg gtcgatgacg cgcccttcct gactgatgta atccttttta
     6001 aactgctccc aggcaggcca ggtacaggct gcctgaacac taaaggcagc caacagcagc
     6061 atcgtcacga ttccactacg caacacattc atcttcaatt actcgttatc cgggttaaga
     6121 cgacgacgac taataattcg cagcagacgc cacagtaccc atgccagcaa tatcacactg
     6181 atagccgcca gcaccgccag aagaatcgga tggtttgcca gcgcatacca caagcgctcg
     6241 aaccacggca gatgacctac gtaataaacg tcgccaacac gtaggctgtt gataccggac
     6301 tcgcggatca ccgcgaccga accgaacatg gtggcgcgtt tgccgctatc gttcaccgca
     6361 tcgttaagca tttcataacc gcgtgggcta tctgccagca gcgcaatcac gctgcgctgg
     6421 tcgttatacg gcgactggaa gccaatcacc gccgccatcg caccggaaga ggtcagcgtt
     6481 gactgggttt ctgccgcgcg atcgctctcg tccggcacaa tgccggggaa cggggtctgg
     6541 cgcatcggtg ttttcaccca gctttcggtc gcctgcacca ataggtcgat ctgcttatcg
     6601 tctttcagtt tgtccgggat accaccaatg atcatgatgt cggcatcttt gccctgaatg
     6661 gtgctgccat catcggtcac cgtcagatta atcgccggga agcccgtctg tgcgccgata
     6721 aaaccaacag tattcagcaa cgtttccatc tgtgcttcgt taggcgtttt cggcatcacg
     6781 gtgatggttt gcgacagatc cgccatccgg ctgaacggga agcccgcgtt agcaaaggcg
     6841 cgtagatccg gcatcgggat gaagtggtaa tacttcgaga agtcgatggt ggaatcgtca
     6901 ccaatcacca catgattctg caccggctgg aaggtaatac agttatccac cgaaccgccc
     6961 ggcatcgggt tcatatactc aaagtcgaag cgcagctggt tggtcgcgcc cagtttcagc
     7021 gccggaatag agacatccgt tttgccatcc agcaaacctt gtaataccgg aatacgcagc
     7081 agcaggcggt tcgcctcctg tttactgctc aggttgaagg attgcaggaa ctggttattc
     7141 aggctgatat ccatccgcga actgtctttc accggcggca tggtgtagcg ataattaata
     7201 tccatatcaa tgccggtact gcgcatcagg tagagatctg gtggcaggtt tagcgaaacg
     7261 ttaatcgctg ctggctcaag accgctggat tgtaactgtt cttcataggt tttcagttcg
     7321 ccaaatgtga ccggacgatc ggtacgtacc cagttcggcg catcgtacgg cttacgcggt
     7381 agcagcggtt tcacttcatt cactaccacg ctttcaccac ggaacagaat gttaccctga
     7441 gcgatacctt tcgctgcctg caacaggtct ttgtcgtcac gaccaaacac caccagcagt
     7501 ttgacgtaag gattctgcgg atggttaatc atctcaatca caggggcttt taccgccgga
     7561 tgatcgcgca ggaagtccgg ccgtttgtcg ttggttgcaa agacaatggc attacgatcc
     7621 ggcagttggt tatagagcac cgggaagttc tgcccacgcc agccagaacg cgaaccaaac
     7681 cacgaggcga caatggcaga ggcttgttgc agcccaacat ccggcgcacc cgcaaagacc
     7741 attggcaagg tgttagtacg gttatcgcgc gggtcaaaga atggcaccgg gaagtgtgac
     7801 aggtcattct tcacattcag ggtctgatag gtcagatcca gtccactgct ccgcccaaca
     7861 tccagccaaa gcgtggtgct ggccgggttt tcgcacacgt cctgataatg gccgacaaac
     7921 tccagccgca cacggttgaa gtcggtaata aacagtgggt taatgggcat ttgcgccagc
     7981 gtttttttac ccaactgttc tttggtcact ggcagcacgc ccatcagctc atcattgaga
     8041 taaaccttta actgcgactg gacaggcagt aacgatggcg atggcgtgta ttcgaggttg
     8101 agcatcgcct tcgtcaccac ttcatcgctg cgcataccaa actcaatgct gccgttcggg
     8161 ttaatgccac gtagcaccat gctgcccggc ggcggtgcaa tttgcgcaaa ggtcagcttc
     8221 acatcacgcg aaggaccgtt ctgcgccacg actggcgcat cagcgccctg cacgcccggc
     8281 atcacttgcc ccacctgcgg attttgttcc gtctgggcgg ctacagctgg ctcagcattg
     8341 atcagtggtt gcgttgctgg cgtcgcctgc gtcatgaaag aggggaacgc actcatcccc
     8401 atagccactg cacaaatcca gaatagtttt cttttcatcg cgttatcatc attgttgagc
     8461 caaagcctga tccgatggtt gtgccgtttc gctccgctcc gggcggcgcg gaataaacga
     8521 tacaacccag gaaaccagag aagtcagcac acggaatatg cccttcaccg aagaaggcgc
     8581 aaactccgcc agatggcggt agccacggaa gccgagcttc agaatatcca gcagactttc
     8641 cagcggctta tcttccgggt agctgtcctg ccagagcgcc catgtatccg cacgggcaaa
     8701 cgtacactgc acaaaatcga tatgttgctg ggtggtgagc ggcattaatt tcagcccaac
     8761 ttcattaccc atcacgcgcg ccacctgggt cgggaagacg tattcctgct gaccgcgttt
     8821 aagcaacaga ttcactttct gcccttccag aatctgcgcc tgaccgttga tcttgatccc
     8881 caaaccaccg tcggagaaat cctgaacggt acatgagaag aggtgaccat cttcgcgggc
     8941 aattgccgcg ggcatcgtca tctccacgcg gtgcgatcgg cgtacctgtt tgctttctac
     9001 cgataccgca actgcgccgc caagaacaat caggttgtag aacacccaca ccatactgac
     9061 gaccacggtg agcatctcgg ttggcgggcc atagaagtag cgccagatgc ctaccgcaac
     9121 gcccaccagg ttgagtagaa caaggaagat gtagggccgc gagatcaccc agtcgacgta
     9181 ctcttcttcc accagtccac ctttggcggt gacgttaaat ttgcctttgt gcgggttaat
     9241 cagcgccacc agcgtcggtg gtgcgatata ccacgccagc accgtttcgt agatttcact
     9301 ccagaaagag tggcgatatt tgccctggat cttcgagttg gtcaggctgg catggatcat
     9361 atgcggcagc acgaataggg cgatcatcaa cgctggcgca tagatgatgt aggcatgaag
     9421 cagcaggaac gccagcggcg cagtcaggaa gatcagccgt ggaatgcccg acaagaagtg
     9481 gaacatggcg ttgacgtagc acagccgctg agcaaacttc agtcctttac cggtgagcgg
     9541 gttatcgaga cggaagattt gcaccatccc gcgcgcccag cgaatacgct gaccgatatg
     9601 cgccgacaga ctttcggtcg ccagccccgc cgcctgcgga atacgcatat acgcggaggt
     9661 atagccacga cggtgcaaac gcagagaagt atgcgcatct tcagtcacag tttcgacagc
     9721 aatgccgcca atttcatcca gcggcttacg gcgaatcacc gcacaggaac cgcagaagaa
     9781 agtggcgtcc cacatatcgt tgccatcctg caccagacca tagaacagcg tgccttcgtt
     9841 cggtgtttta cgaaaacgcc ccaggttgcg ttcaaacggg tctggcgaga agaagtggtg
     9901 tggagtctgc atcatcgcca gctgtttctc tttcaggaac cagcccatgg tcatttgcag
     9961 gaacgatcgc gttggtacgt ggtcgcagtc gaaaatcgac acgaactcgc ctttggcata
    10021 tttcagcgca ttgttgatgt tgcccgcttt cgcatgttca tgagtggtac gggcgatata
    10081 tttcaccccc acgttttgcg caaactggcg aaactcttcc ctaccgccgt catcgaggat
    10141 ccagatgttc agcttgtctt tcggccagtc gatacccagc gaggcgtaaa tagtattttt
    10201 caccacgttg agatcttcgt tgtaagtcgg gacaaagata tccaccgacg gccacagcga
    10261 catatctttc ggcaatggca ccggctgacg attcagcggc catacgacct ggaagtagcc
    10321 gagcaccagc acaatccacg cgtacgtttc agcgaagagc agaataagcc cgcacaccag
    10381 gctgaccgga tcgtcccagt tcagcgtaga ggtgtaacgc caccagatat aacggcaaga
    10441 aacggtcagc gacagcacaa tcaacattag cgccgagaag cgccccggca tccgccgtac
    10501 gatcagcgct acaccccaca gcagcatcag gaagataaac tgcgccagcg ggttaaacgg
    10561 ttgagtaacg cagattaacg ccagaatcag cgagaaggtg acgatgatac cgaggatcaa
    10621 ccgccgcgcc ccggcactca aatgaccgag ctcttttttc tcatcaagat gctgtgtttt
    10681 atggctaacg cgctcaggca gctcgttcat ccattgatgg taacgtccac gaatattctg
    10741 cagacctgaa aatgcccgcc tgcgcggttt cggcgtttct ttgcgcgatg taccgatcag
    10801 taaccagcat gtttgaatga gataacggac cgggtccagc ggacgcggac gcgaggcgtt
    10861 gatatgcgga tacaggtttt tatgttctgc gcgaatacgc tgccagcgcg ggtgctccag
    10921 cggaataaaa atccaggcca ggatcatcca gaaacagccg agcgtcgcgc tgaaagccga
    10981 cgcaccgtga cgacgataat cgcgataacg cccgataagc cgcgcgttga ccggcgggat
    11041 aagcaaccac cgggtcagga tactcatgat gcactcccga ctggcgtttt cagcccggag
    11101 tagttcaaca ggcaccagtt cgccagcgtc agtatctctt cagccgccag cgcatcactg
    11161 cgatattcac ctactggttg cttagccgcc aggcattcag ccatcgcttc atcacgatga
    11221 atgagcatcg gcagtaatcg gcgctggctt tgcaaccaaa gctggtaaat atcgtcctga
    11281 acctgactgc caatacggaa gtcattaatc aaaatatgtg cgccatccgg cagcgcttgc
    11341 tgatgcagtc ggatatggca gttggcatcc acattgacga tagccagcga gtgatcgcac
    11401 aaactcagca gctggtgggt tatctgcgag gcatcacgcg gtaagtcgat taaaatccac
    11461 tggtaacgcc cgctggcttt tagttgctgt aagctggagc aaatatcgct cagctgggtt
    11521 tgccagtgct gtggattttc ttgttcttca atggataact gaccaaaagg cagcaaatcg
    11581 agctgcgagg tgtagcgcaa cccggcgtca cgccagtcct ggccatccag catcgctctg
    11641 gcccagccct gacggtgggt aaaatcaacg ttaaatgaca ggcgcaacaa gttgtccggg
    11701 caggcatcga ccaccaggac attttctccc aacatttgta atgaccaggc taatgcggcg
    11761 gtgatggttg ttgtccccac gcctccccgc accccctgca atcccagtac ggccattcac
    11821 caatccccta ctattgttgc gcaaactctg ccagcaacgg ccagcgtttt aatgccgcgg
    11881 ccaactgttc gcgttgggaa atatcggcat aatcaatatc aggcagtgaa aatgcctgct
    11941 ttaacgccaa aatatcattc tggaagatat agcctatcgc gggatcaggc agagtatctg
    12001 gttcattgtt attcatttgc ttgatcccta tgaaataacc gtaccgttca tgagacaatt
    12061 ctaaccgtga gcatttcgcc ggtttattgt ttacggcgag acgggaattt gctcactttt
    12121 tgactttccg ttacgcaaca gaaaaagtta aaaacgaaaa gactaaaatc acacgtcttg
    12181 tgaccttcta aattgattca catgctaaat ctgataagtt ttaatttcaa tggtaggttt
    12241 atttcttagc tttcgctagt aaactgataa tcagacaaaa tggtgacatg agggacattg
    12301 tggaccctgt attctctatc ggtatctcat cattatggga tgagctgcga catatgccag
    12361 caggcggcgt ctggtggttt aacgtcgatc gccatgaaga tgctatcagt ctggcgaatc
    12421 aaacaattgc atcccaggct gcaaccgcac acgtcgcggt cattagcatg gacagcgatc
    12481 cggcgaaaat ctttcaatta gatgattctc aagggccgga aaaaataaaa ttattttcaa
    12541 tgctaaatca tgaaaaaggt ctatactatt tgacccgtga tttgcagtgt tctattgatc
    12601 cccataatta cctttttatt cttgtttgcg caaataacgc atggcaaaac attcctgccg
    12661 agcggcttcg ctcatggttg gataaaatga ataaatggag taggttaaac cattgttcgc
    12721 ttttggtaat taatcccgga aataataacg ataaacaatt ttcattgttg cttgaggaat
    12781 accgttcact ttttggtctt gccagtttgc gttttcaggg tgaccaacat ttgctggata
    12841 ttgccttctg gtgcaacgaa aaaggggtca gcgcccgtca gcagcttagc gttcagcaac
    12901 aaaatggtat ctggacatta gttcaaagcg aagaggcgga gatccaacca cgcagcgacg
    12961 aaaaacgcat tctgagtaat gttgctgtac tggaaggtgc gccgccgcta tcggaacact
    13021 ggcaactgtt caacaataac gaagtcctgt tcaatgaagc ccgtaccgct caggcggcga
    13081 cggtggtctt ttctttacag caaaatgcgc aaatcgagcc actggcccgc agcattcata
    13141 ccctgcgtcg ccagcgcggt agtgcgatga aaatcctcgt gcgggaaaat accgctagcc
    13201 tgcgcgccac cgatgaacgt ttgttattgg cctgcggtgc aaatatggtt attccgtgga
    13261 atgcgccact ctcccgttgt ctgacgatga tcgaaagcgt gcaagggcag aagtttagtc
    13321 gctatgtgcc ggaagatatc actaccttgc tgtcaatgac ccagccgctc aaactgcgtg
    13381 gtttccagaa gtgggatgtg ttctgtaatg ccgtcaacaa catgatgaat aaccctctat
    13441 tacctgccca cggtaaaggc gttctggttg ccctacgtcc ggtaccgggt atccgcgttg
    13501 aacaagccct gacgctgtgt cgccctaacc gtaccggcga tatcatgacc attggcggta
    13561 atcggctggt gctgtttctc tcattctgtc ggattaacga tctggatacc gcgttgaatc
    13621 atattttccc attgcctact ggcgacattt tctcaaaccg tatggtctgg tttgaagatg
    13681 atcaaatcag tgccgagctg gtgcagatgc gcttgcttgc cccagaacaa tggggcatgc
    13741 cgctgccttt aacgcaaagt tctaaaccgg tcatcaatgc cgagcacgat ggtcgccact
    13801 ggcgacgaat accagaaccc atgcgactgt tagatgatgc tgtggagcgc tcatcatgat
    13861 gaccatcagc gatatcattg aaattattgt cgtttgcgca ctgatatttt tcccgctggg
    13921 ctatctggcg cggcactctt tgcgacgcat tcgcgacacc ttacgtttgt tctttgctaa
    13981 acctcgttat gttaaaccgg ccgggacgtt acgccgcacg gaaaaagcca gggcaaccaa
    14041 aaaatgactc aatttacgca aaataccgcc atgccttctt ccctctggca atactggcgc
    14101 ggcctttccg gctggaactt ctattttctg gttaagttcg gcctgttgtg ggcgggatat
    14161 cttaacttcc atccgctcct caatttggtg tttgccgcgt ttctgctgat gcccattccg
    14221 cgctacagcc tgcatcgctt gcgccactgg attgccctgc cgatcggctt tgctttgttc
    14281 tggcatgaca cctggttgcc tggcccggaa agcataatga gccagggttc gcaggtggcg
    14341 gggttcagta ccgattattt aatcgacctt gtcacacgct ttattaactg gcagatgatt
    14401 ggggccattt ttgttttatt agtggcctgg ttattcctgt cacaatggat tcgcattacc
    14461 gtttttgtgg ttgccatact gctatggctg aacgtactta ccctggcggg accaagtttc
    14521 tccttgtggc cagccggaca accgacgacc actgtaacaa cgacgggtgg taacgcagcg
    14581 gcaaccgttg cggcgacggg tggcgcaccg gtagtgggtg atatgcccgc acaaactgca
    14641 ccgccaacaa cggcgaacct taacgcctgg ctgaataatt tctataacgc ggaggcaaaa
    14701 cgtaaatcga ccttcccgtc ttcgctgccc gctgatgctc agccatttga actactggtg
    14761 attaacatct gttcgctttc ctggtcggat atagaagccg ccgggttgat gtcgcatcca
    14821 ctgtggtcgc atttcgatat tgagttcaag aactttaact ccgccacctc ctacagtggc
    14881 ccggcggcga tccgtttact gcgcgccagc tgcgggcaga cttcgcacac taatctgtat
    14941 caaccggcaa ataacgactg ctatctgttt gataaccttt cgaaactggg ctttacccag
    15001 cacctgatga tggggcataa cggccagttc ggcggttttt tgaaagaagt tcgcgaaaat
    15061 ggcggcatgc agactgaatt gatggatcaa acaaatctgc cggttatttt gctgggcttt
    15121 gatggttcgc cggtttatga cgataccgcc gtgcttaacc gctggctgga cgttaccgaa
    15181 aaagataaaa acagccgtag tgccacgttc tacaacacgc ttccactgca tgacggcaac
    15241 cattatccgg gggtcagcaa aacagcggat tacaaagcgc gggcgcagaa attctttgat
    15301 gaactggacg ccttctttac tgaactggag aaatcgggtc gtaaagtgat ggtggtcgtg
    15361 gtgccggaac acggcggcgc gctgaagggc gacagaatgc aggtatctgg cctacgtgat
    15421 atccctagcc cgtctatcac agacgtcccc gttggggtga aattcttcgg catgaaggca
    15481 ccacatcagg gggcaccgat tgtcattgac caaccgagca gcttcctggc tatctccgat
    15541 ctggtggttc gcgttcttga tggcaagatt ttcaccgaag acaatgttga ctggaaaaac
    15601 tcaccagtgg gttgccacaa acagcaccgg tctccgagaa ctcaaatgca gtagttattc
    15661 aataccagga taaaccgtac gttcgcctga acggcggcga ctgggtgcct tacccgcagt
    15721 aagcacgaaa aaggccgcag agtttaacct ctgcggcctg atccgggcgc aaattgccat
    15781 tacggcagcc tgacgcccgc atgacacgtt acttccgctt attcagccag ttcacgatca
    15841 tactggcaag aatgccagca atgaccggag ccgctaaatc atgccagaag gccatgccca
    15901 gttctgcgag cgtcatatag ccgcctgtgt tgtaatgaca acgtttcgcg gctattcttg
    15961 agttgttcag gttcaagaat ggcccccgtg atgttgtcag gtgcatacct gcaacgtgcg
    16021 ggggttttct ctctccagca accaatgcca ccagggataa agcccccgca acattgcgcc
    16081 tcaccggaac ccttccggct tgccgctgat tctacgactg tttttctgtt ccggaatccg
    16141 tcacgtcttc actctgtgct gcgcattccg cagaatatta tccgcttgca accgttgcat
    16201 ttttcttacg aaaaaggccg cagagtttca cccctgcggc ctggtccggg cgcaaattgc
    16261 cattacggca gcctgacgcc cgcatggcac gttacttccg cttgttcagc cagttcacga
    16321 tcatactggc aagaatacca gcaatgaccg gagccgctaa atcatgccag aaggccatgc
    16381 ccagttctgc gagcgtcata tagccgcctg tattgtaatg acaacgtttc gcggctattc
    16441 ttgtattgtt tggatgcaag aatggccccc gtggtgttgt caggtgcata cctgcaacgt
    16501 gcgggggttt tctctctcca gcaaccaatg ccaccaggga ttaagccccc gcaacattgc
    16561 gcctcaccgg aatccttccg gcttgacgct gattctacga ctgtttttct gttccggaat
    16621 ccgtctcatc ttcactctgt gctgcgcatt ccgcagaata ttatccgctt gcaaccgttg
    16681 catttttctt acgaaaaagg ccgcagagtt tcactcctgc ggcctggtcc gggcgcaaat
    16741 tgccattacg gcagcctgac gcccacatga cacgctactt ccgcttgttc agccagttca
    16801 cgatcatact ggcaagaatg ccagcaatga ccggagccgc taaatcatgc cagaaggcca
    16861 tgcccagctc tgcgagcgtc atatagccgc ctgtgttgta atgacaacgt ttcgcggcta
    16921 ttcttgagtg gtctaggttc aagattagcc cccgttatgt tgtcaggtat atacctgcaa
    16981 cgtgcggggg ttttctctct ccagcaacca atgccaccag ggataaagcc cccgcaacat
    17041 tgcgcctcac cggaaccctt ccggcttgcc gctgatttta cgattgcttt ttactgtcag
    17101 cacccggcaa tcttcctcat tgcgcgatgc cttccagctt aatttcttta tttgcaatag
    17161 ttgcagttca tatccgggct gactccagac tggtagtgaa atgcgcaatt ttgtagaatt
    17221 gcgcctcctc gcgacctccc cagtgaatct gttgcctcat tttttacata ctgcgtattc
    17281 gacttctcca cctgttgcgc aagagaaact gggtttattc atttttgcga ggccgacttc
    17341 tttctggaca ggactttatg cagcacaaca cactatcgaa acacaatcag aaattgccgt
    17401 ttacacgcta cgacttcggc tgggttttat tatgcatagg catggcgatt ggtgccggaa
    17461 ccgtgctgat gccagtacaa attggcttga agggaatttg ggtatttatt accgcagcga
    17521 tcattgctta tcctgccacc tgggtagtgc aggacattta tttaaaaacc ctttctgaaa
    17581 gcgattcctg taatgactac accgatatta tcagtcatta cctggggaag aactggggaa
    17641 ttttcctcgg ggttatctac tttttgatga ttatccacgg gatttttatc tactctctct
    17701 ccgtggtttt cgacagcgcc tcgtacctga aaaccttcgg tttaaccgat gccgatcttt
    17761 cacaatctct actttataaa gtcgctattt tcgccgtact ggtggcgatt gcgtctggtg
    17821 gtgaacgatt actgtttaag atttccgggc caatggtggt ggtcaaagta gggattattg
    17881 tcgtgttcgg ttttgcgatg atcccgcact ggaatttcgc caatataacc gccttcccgc
    17941 aagcctccgt ctttttccgc gatgtcttgc ttaccattcc attttgcttc ttttctgcag
    18001 tatttattca ggtacttaac ccaatgaata ttgcctatcg taaacgggaa gcggataaag
    18061 tactggcaac ccggctcgcg ctgcgtaccc accgaattag ttatatcacg ctcatcgcgg
    18121 tgatcctgtt ttttgccttt tcgtttacct tctcaattag ccacgaagaa gccgtttctg
    18181 cctttgaaca aaatatctca gcactggcgc tggccgcgca ggtgatccct gggcatatca
    18241 ttcatatcac ctctacggtg cttaatatct ttgccgtact gaccgcattc tttggcattt
    18301 atctcggttt ccacgaggcc attaaaggca ttattctcaa tctgttaagc cgaattattg
    18361 ataccaagaa aattaactca cgcgtgctga ctctggcgat ctgcgctttt atcgtcatta
    18421 cgttgacgat ttgggtttcg tttcgtgtat cggtgctggt gttctttcag ttgggaagcc
    18481 cgttatatgg tattgtgtcg tgcctcattc cgtttttcct gatctataaa gtcgcacaac
    18541 tggaaaaact tcgcggattt aaagcctggc tgattctgct gtacggcatt ttgctatgct
    18601 tgtcgccact gttgaagctg attgagtaaa ccggagcgca tggccccggt tttgtgagtt
    18661 aacgctgcgg attttcatcc tgatcaacag caaaacaagc taccagttga ccgccgtagt
    18721 cttttagctg cggctgcaac tgggtgcagg ggccgaagcg ccgacgacag cgggcgttga
    18781 aggcgcaacc cggcggtgga ttcagtgggc ttggtagttc accgctgagc ttgatgcgct
    18841 cgcggcgatc gtccgggttc aggcgcggcg tcgcggaaag tagcgcctga gtgtacggat
    18901 ggcgcgggtt attgaagatt tggtctttcg ttcccttctc cacgcagcgg cccaggtaca
    18961 tcaccatcac ttcatcagca atgtgctcca ccaccgacag gtcgtgggag ataaagacat
    19021 aagacagccc caactcctgc tgcaaatcca tcatcagatt cagcacctgc gcgcgcactg
    19081 aaacatccag cgcggaaacc ggttcatcgg caatcaccac atccgggtcg agcatcagac
    19141 cacgggcgat ggcgatacgc tgacgctgac cgccggagaa catatgcgga tagcggtcat
    19201 agtgctcggt tttcaggccg actttcgcca tcatcgacag ggctttttcc cgacgctgtt
    19261 ctttgcttaa gctggtgttg atcagcagcg gctcttcaag aatttgcccg acttttttac
    19321 gcggattcag cgaaccgtaa gggttctgga agacgatctg gattttctgc cgacgcagct
    19381 tctgcgcctg cggatcgtgc ttaagcagat cctgcccctg gtaatacagc tcgccaccgg
    19441 tgggcatttc aatcatcgtc agcaaccgac cgagggtcga tttaccgcag ccagattcgc
    19501 ccactactgc cagcgttttg ccacgttcaa ggttaaacga aacgccatcc agcgctttaa
    19561 ccagacgttc cggcgcgaac atgcctttct tcaccggata atgttttttc aggtcgatag
    19621 cctgcaacag cggttgttgc gaggtggcct cttgcgtact catagtgtcg gcctcccggc
    19681 atcatcaagt gggtaatggc atttggactg acgcccgtca gcgagcatat tcagcgccgg
    19741 ttcttcagcg cgacatctgt cagtggcata ggggcagcgc gggttaagca ggcagccgtt
    19801 cgggcggtcg tacttgccgg gaacgacacc tggcaacgac gccagacgtt ctttgtcctg
    19861 agcaaattct ggcagcgcac gcagcaatgc ctgagtatac gggtgacgcg gcgcatggaa
    19921 gatggcgtgc gcatcaccgg tttccaccac ctggcctgca tacatcacga tgattttatg
    19981 tgccgcttcc gccaccagcg ccaggtcatg ggtaattaac accagcgcca tgttctcttt
    20041 ctgctgtagc tccagcagta gttcgatgat ttgcgcctga atggtcacgt ccagcgcggt
    20101 ggtcggttca tcggcaatca gcagttttgg ccgacaggca atcgccatgg cgatcatcac
    20161 gcgctggctc atgccgccgg aaagctgatg cgggtaaaca tccagacgcg atgccggatc
    20221 gggaataccg acctgattca gcaggtcaat cgcccgctga cggcgagtgc tcttattacc
    20281 gccctgatgc accttaatcg cttccatgat ctggaaaccc acggtgtagc acgggttaag
    20341 gctggtcatc gggtcctgga agatcatcgc cacttcggca cccaccaggt tgcggcgctc
    20401 tttttctgat atacgctgca aatcctgacc gttaaacacc agcttttccg ccattacgcg
    20461 gcccggataa tcaatcagcc ccataatcgc cagtgaactg accgacttac cggagccaga
    20521 ctcacccaca atcccgacca cttcgccctg ttttacgctg tagctgatgc ggtctacggc
    20581 gcggaacggt gcgctttcgt cgccgaaatg caccgataat ttatctacat ttaataacgc
    20641 catctcgaac ctcttactgc ttcagtttgg gatcgagcgc gtcacgcaga ccgtcaccca
    20701 tcaggttaaa tgccagcacc gtcagcagga tcgccagacc cgggaaggtc acgacccacc
    20761 aggcgctttg cgcgaactgc aacacgtcgg agagcatggt gccccactca ggcgttggcg
    20821 gctgtgcccc catgccgagg aaaccaagag cagccatatc gagaatggcg ttagagaaac
    20881 cgagcgacgc ctgaacaatc agcggcgcaa ggcagttcgg gaagatgtta ataaacatct
    20941 gacgcatcgc cccggcaccc gccacgcgag acgcggtgac gtaatcgcgg ttaacttcca
    21001 ccagcacggc ggcgcgggtt aagcgcacat agtgcggcag ggcaacgaag gtcagtgcca
    21061 gcgcggcgtt accaatcgac gggccgaaaa ttgccaccag caccagcgcc agcagcagac
    21121 ttggcagcgc cagcatgata tcgaccacgc gcataatgat gttatcgacc aggccgccaa
    21181 agtaaccggc gatcaggccg agaataacgc ccataatcag cgataacaca actaccagac
    21241 agccaaccag cagcgacagg cgcgcaccgt acatcaggcg cgacagcaca tcacggccta
    21301 cgtcatcggt gcccagcaag tgcgccatgc tgccgccttc ctgccaggct ggcggggcga
    21361 gcagtgcatc gcggaactgt tccgccgggt tatagggtgc aatccagttg gcaaagatcg
    21421 cgatgaacag cacgatgacg acgtaaacca gcccgacgac cgcgcctttg ttgcgtttaa
    21481 aatagtgcca gaactcctgt aacggggtca tcggcaccgg tgcgctaatc actttatttt
    21541 cagtaacctg tgacatgatg tccccttact tcttatgacg aatacgcggg ttcaccacgc
    21601 cgtacagcag atcgaccagc aagttgacga ggataatcat cgtcgccacc agcaatacgc
    21661 cgccctgcac taccggatag tcgcggcgtt gcagtgcgtc aatcaaccag cgtcccagac
    21721 cgggccacga gaagatggtt tcggtcagaa tcgcccccgc cagcaatgtt cccacctgca
    21781 ggccgataac ggtcaccacc ggcagcatcg cgttacgcag cgcatggacg ataatcaccc
    21841 gcatgcgggt tagccctttg gcgcgcgcgg tgcggatgta atcctcgccc agcacttcca
    21901 gcatcgagga gcgtgtcata cgcacaatga ccgccagcgg aatagtaccc agcacaatgg
    21961 caggcaagat catatgggcg acggcatcga taaagttgcc gtcttcaccc cagatggcgg
    22021 tgtcgattag cataaaaccg gttaacggat tggagtcatc gaggaacacc atatcgctca
    22081 cgcgaccgga gacgggcgtc aggttccagt gcaccgaaac cagcatgatc agcatcatgc
    22141 cccaccagaa gatcggcatc gagtagccgg tcagcgccag gccaactgca gtgtgatcga
    22201 aaatggaacc gcgtttaacc gcagccagca caccaaccgg aataccgact gccgtagcaa
    22261 aaatcatcgc gcagacgcca agttccagcg tggcctgaaa gcgcggcacg aactcttccc
    22321 atactggaat tcggcttttc attgaaatgc ctagatcgcc gtgcattacg ccccaaatgt
    22381 aatggagata ctgctgccac atcggtttat ctaagccgag ttcagccagc agctgcgcgt
    22441 gacgctcagg ggagatccca cgttcgcccg ccatgatcat caccggatcg cccgggatca
    22501 tgtggacaaa ggcaaatgtg agaagggtaa taccgataaa cgtggggatg acgagtccca
    22561 aacgtcggag aataaactgc aacataaccc ggattctcta taatgacccg cagcctggaa
    22621 ctgcgtgtct gtattgctca caaatcaatt cccgcccttg tcggacagag aatgaagtat
    22681 tgctgccgga tggcgctgca cgcagaggca cagcgccacc gggtatggct tttaattatt
    22741 cgatagagac gttttcgaag tgatgtttgc ctaatggatc aaccacatag cctttaactt
    22801 ctttacgtac cggttcaaac acggtggagt gagcgatgat cagtgccgga gcctgatcgt
    22861 gcatcaccac ctgtgcttgt ttgtacagtt caacgcgttt gttatggtcg tcggtagcac
    22921 gcgccggttg aatcagatct tcaaacggtt tgtagcacca ttttgagtag ttggagcctt
    22981 gttcagaggc ggcgcagctg aacagggtgg cgaagaagtt atccggatcc ccgttatcgc
    23041 cagtccagcc catcattacc gtctggtgct cgccatcttt cgcacgcttg aggtactcac
    23101 cccattcgta ggtgacaatt ttggcctgca cgccgacttt cgcccagtct gcctgaatca
    23161 tctccgccat gcggcgagcg ttcgggttat acggacgttg taccggcatc gcccacaggt
    23221 cgatggagaa acctttttcc agacccgctt ctttcagcaa ggctttcgct ttttcaggat
    23281 cgtaggtgta gtcctgaacg tcgtcgttat agccccacat ggttggcggg atcaggtttt
    23341 tcgctgatac acccgcgccc tgataaaccg ctttgatgat cgcgtctttg ttcaccgcgt
    23401 aggtcagagc ctggcgaact tttacgtcat cgagtggttt tttctgcacg ttatatgaaa
    23461 gatagccgac gttcagcccc ggcatttcca tcagattgat ggatttatcc tgcttcatgc
    23521 gagcgatatc tgccgggttc gggtacggca tcacctggca ttcattcttc tgcaatttcg
    23581 cgtaacgcac ggaagcgtca ggggtaatag agaagaccag cgtatcgatc tgcggtttgg
    23641 tgccccagta gccatcaaac gctttgtagc ggatacggga atctttttga tactgctgta
    23701 actggaacgg accggtgccg attgggttga ggtccagttt ttccggtgta ccggctttca
    23761 tcatcgcatc agcatattct tttgacagaa tagaggcgaa gtccattgcc aggtcagcga
    23821 ggaacggcgc ttccgggcga gtcagcacaa actgaactgt gttgtcgtcc acctttttca
    23881 cttcgctgat cagctccggc aagcccatgc cttcgaagta ttcgtagctg ccgccagaaa
    23941 ctttatggta cgggttttgc gcgtttttct gacgatcgaa cgagaacacc acgtcatcgg
    24001 cgttcagttc acgcgtcggt ttgaattctt tattgtcgtg ccacttcaca cctttacgca
    24061 gatggaaggt ataggtttta ccgtcttcgc tgacttccca cttttcagcg aggcccggaa
    24121 tcacttcggt ggtgccgatt ttaaattcaa ccaggcggtt ataaagcggt acggaagagg
    24181 cgtcataggt ggtaccggag gtaaacagct gcgggttaaa cccttccgga gatccttctg
    24241 agcaataaac cagagtttta gcctgaacac ttgctgcgac ggtcatagcc accaggctga
    24301 gaccaagctt cagcatccct gactttttca aggaaatacg cattattctg ctccaattgt
    24361 gatgtttgtt gttttaaccc tttgcagtgg gttgtcgctg cctgaccttt tttgtttttt
    24421 gcccggtcgg gtcaacgtta tgaggtgggg atgccgtact aattaacatc agtgtaacag
    24481 tgcggatcct ccataaaatg cccctcgtga cctacaatct gtcaacagaa tgtgaaaacg
    24541 tcaatacagc caaccgggat ttacaccaac ggtgagaatc cacaaacaaa gattaaaaaa
    24601 acttcaaaca gctatttgca gcagagaaat ttgtgctact ccaacatgac cagaacaatc
    24661 agcttaatat ttagcaacat ttggtgatta agttttatgc atttagtgaa aaaaatctga
    24721 ggaaaaggtg gatatctgag cgattaatac catgaacgct aaaacgcaca gcggaaaatg
    24781 ccagcgccag ccataagtta cgaaaaaaaa gatttaagca aatataaaaa aagacaatgg
    24841 tttctgtcac aaatccgtta atggatagtg agatatgggg cgcaaatttg gcagggaaat
    24901 gtgtggcgtg taggtcgaat aaggcgttca cgccgcatcc gacaataagt gcggtgccgt
    24961 tgcctgatgc gacgctgacg cgtcttatca ggcctacgtc gagtcgaacc tccccgcccc
    25021 cggaggttct catcctcatg agctacaaat gcaaaaaagc ctgctcgttg agcaggcttt
    25081 tcgaatttgg tcggtgatag aggattactc gccacttcgt ggctcgccct gcgggccgtt
    25141 gctggcgcaa cgttctctcg cttcgctcga gtcgaacctc cccgcccccg gaggttctca
    25201 tcctcatggg cttcaaatgc aaaaaagcct gctcgctgag caggcttttc gaatttggtc
    25261 ggtgatagag gattcgaacc tccgacccct tcgtcccgaa cgaagtgcgc taccaggctg
    25321 cgccaatcac cgaatgcggg gcgcatctta ctgcgcagat acgccctcgt caatccctta
    25381 atagcaaaat gccttttgat cggcgagaaa gtcagcaggc cgcttagtta gccgctgcca
    25441 cttttgtctg cgggatgtga caccagttgt tgttttcgtt aattccacca tccggtgaag
    25501 tatagccaag acaacccatg atggtgtcgt acagctctac atgacggcgt ggcactttca
    25561 tgtcggcttc ttttttcagc tgcgcaaatg cctgagcatt ggccggattt tccagatatt
    25621 tatctgacat ccagaccatc atcggtacgc ggaactgctc cggcggtgcc agttcacgcg
    25681 gcgtgccgtg caggtgctcg cgttcattaa ttgactcacc gtggtcagct gcgtagaaca
    25741 caatcgcttt cttatcgcga acctgatcaa tcacgctgga gataaagtga tccacatagg
    25801 tcaccgagtt gtcataggag ttgatcatct gcgctttggt acagccgcta tcaacaccaa
    25861 tacattccgg cttccactgc gcgaagctac gcggataacg ctgggtgtag ttaaaatgcg
    25921 aacctttggt atgcagaatg atcagatgct taccatccgg gttgcgccct agcgattgct
    25981 gcatttcgtc taccagcaac atatcatcta ccggcttgcc acgattacgt ggctccgcac
    26041 caatctgctc acgataagca atgttgtccg ccatcgtgtt gctgtagaac cacatttcgc
    26101 tctgcatagc gtagaggtca gaactgaatc ctaactgctt cagaaccgcg aaaatgttct
    26161 gttcttttaa tgtgcgctgc ggattatctt ccgcgccccc ctgacgtaca aacatgcaac
    26221 gcagtgagag tttggttgcg gtatcacagg agtaaccacg gaacgccgcc agatttttct
    26281 cctgggccag tttcggcgtg gtattacgct catagccgaa aatgcccata tggtcccaac
    26341 gcgtggtttc accgatgata aacacgacat aagtgtcatc aacgttttgc ggtgcctgat
    26401 aagtgaattt cttcgccgga ttaagcaatg aattattatc ggaagattca tcgacccgcg
    26461 cccaggcata cagccccaac gcagaaagcc agtttgatgg cagataagag ttcgctacga
    26521 caccgccata actcggcaaa tcaacgccgg tcgccctctc cactttcttc tgctggatat
    26581 ccagcaaacg aatcggtgcc caaaccataa tacccgccag tacgacgacc gccaggctgc
    26641 gaatacgctg ccctggggtt cgcagttgtc ggagcaaggt gtagcgacag cggttattcc
    26701 agataaggat aagaggcaat gcactaacgg cgattaacca gagaataaag ttcagaccaa
    26761 caacttcttt tgacaggtcg atatcggtgg tcatgacgga agcgatgatg ccataaccaa
    26821 tgaccacatt aaggaaggtc atgtaatagc tggcacctgc ggaaaagagc accaccagcg
    26881 acgccagaat acgccagctg cggcggccaa acagcgatag aagacgtagt aaaaagaagg
    26941 tcaccagtac ggtggcggcc agttcaacaa cagcagaaac gcctttccag acggtaaaat
    27001 cgtgcgcata gctgccgaag cggcgataaa aaaccgcgcc attcataaaa aggccaatat
    27061 agattgcaag caaaaagctc agcttctgct gtgtaatcga tttgatgtat ctcatgcaaa
    27121 caaaccctgg acaaacaggt gataacaccc tgcttagcgg cttttcaggc ccggcagggg
    27181 aaaagtgcgg aggtagtcag ataagtaaaa tctacacgcg cgaataagta gaccacagca
    27241 aagcgaaaaa gtgtggggaa agagtgtgca tgaagcacaa atttacaaaa atgcaagttt
    27301 gaaaagagac tttagcgtta caaaaagcag taaaaagggc cagttaactg gcccaaattt
    27361 tacgagtcat tgtttataca tttcgttgat gcgtttgatg cacacggaag ctgaagccca
    27421 gtagctcgcg gctgagcatt aaagggagcc atgcgcctca cgcaacattt tctgctctgg
    27481 ctgacgaatt gtcgtagaaa gcgccaatga cagaatcagc agggcgaaaa tcacgtagaa
    27541 ggtcacatag aagccgccaa acagtgaggc gataatcgaa ccaaaaatgc taccgatacc
    27601 gaaaccgagg taaatcacac cgtagttttt cgccaggtta ttgaggccga agaactcact
    27661 gaccagtgac ggaaagacgg taatagtgcc gccaaagtta aatgccacgc aggcaatcgc
    27721 tgcaaagaac gtcactgcat tcagtggtgc aaacagcagg gccgccatac ccactagcga
    27781 aatcacctga ccaatggtga taacacggat acgggcgatt ttgtcagaca gtatccccag
    27841 aaccagacga cctgaaaggt tggcgatgga aataacagtg actgcattag ctgcggaaac
    27901 cacatcaagg tgtgccagac tttgggcgat atctttcgct accccaatca cgtacaggcc
    27961 gctcatgcag gcagtcagga acattaccgc taacatccag tactgcggtt tacgcatcga
    28021 ctctgccagc gtgtaatctt tctccaccac accattgctg gttttcactt cctgttttgg
    28081 tgcgtctttc attaacgttg cgccgaaaac aatcatcacc agcgcaatcg ctccccaaat
    28141 cacaaaggtt ttttccagac ccaccgtttc cagcagctgc gtgtcgataa atttgaaacc
    28201 caggctaccc agaccataag aaccgatagc gaacgcggat atcag
//
LOCUS       DAEVZA010000055        28195 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEVZA010000055 DAEVZA010000000
VERSION     DAEVZA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.055"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..726)
                     /gene="glsB"
                     /locus_tag="LAF92_002413"
     CDS             complement(<1..726)
                     /gene="glsB"
                     /locus_tag="LAF92_002413"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBI9791408.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSG"
     gene            complement(790..2178)
                     /gene="sad"
                     /locus_tag="LAF92_002414"
     CDS             complement(790..2178)
                     /gene="sad"
                     /locus_tag="LAF92_002414"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBI9791409.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2279..3160
                     /locus_tag="LAF92_002415"
     CDS             2279..3160
                     /locus_tag="LAF92_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9791410.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            3238..4352
                     /gene="yneK"
                     /locus_tag="LAF92_002416"
                     /pseudo
     CDS             3238..4352
                     /gene="yneK"
                     /locus_tag="LAF92_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            4502..5692
                     /gene="ydeA"
                     /locus_tag="LAF92_002417"
     CDS             4502..5692
                     /gene="ydeA"
                     /locus_tag="LAF92_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBI9791411.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSGHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(5717..6382)
                     /gene="marC"
                     /locus_tag="LAF92_002418"
     CDS             complement(5717..6382)
                     /gene="marC"
                     /locus_tag="LAF92_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBI9791412.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6594..7028
                     /gene="marR"
                     /locus_tag="LAF92_002419"
     CDS             6594..7028
                     /gene="marR"
                     /locus_tag="LAF92_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBI9791413.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7048..7431
                     /gene="marA"
                     /locus_tag="LAF92_002420"
     CDS             7048..7431
                     /gene="marA"
                     /locus_tag="LAF92_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBI9791414.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7463..7681
                     /gene="marB"
                     /locus_tag="LAF92_002421"
     CDS             7463..7681
                     /gene="marB"
                     /locus_tag="LAF92_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBI9791415.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(7738..9177)
                     /locus_tag="LAF92_002422"
     CDS             complement(7738..9177)
                     /locus_tag="LAF92_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBI9791416.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(9202..10875)
                     /locus_tag="LAF92_002423"
     CDS             complement(9202..10875)
                     /locus_tag="LAF92_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBI9791417.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(10931..11242)
                     /locus_tag="LAF92_002424"
     CDS             complement(10931..11242)
                     /locus_tag="LAF92_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBI9791418.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(11270..12592)
                     /locus_tag="LAF92_002425"
     CDS             complement(11270..12592)
                     /locus_tag="LAF92_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBI9791419.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(12707..13018)
                     /locus_tag="LAF92_002426"
     CDS             complement(12707..13018)
                     /locus_tag="LAF92_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBI9791420.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            13217..13915
                     /locus_tag="LAF92_002427"
     CDS             13217..13915
                     /locus_tag="LAF92_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI9791421.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(13960..14859)
                     /gene="eamA"
                     /locus_tag="LAF92_002428"
     CDS             complement(13960..14859)
                     /gene="eamA"
                     /locus_tag="LAF92_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBI9791422.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            15054..16241
                     /gene="ydeE"
                     /locus_tag="LAF92_002429"
     CDS             15054..16241
                     /gene="ydeE"
                     /locus_tag="LAF92_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBI9791423.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            16368..16463
                     /gene="mgtS"
                     /locus_tag="LAF92_002430"
     CDS             16368..16463
                     /gene="mgtS"
                     /locus_tag="LAF92_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBI9791424.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(16682..17572)
                     /gene="dgcZ"
                     /locus_tag="LAF92_002431"
     CDS             complement(16682..17572)
                     /gene="dgcZ"
                     /locus_tag="LAF92_002431"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBI9791425.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(17827..18219)
                     /gene="ydeI"
                     /locus_tag="LAF92_002432"
     CDS             complement(17827..18219)
                     /gene="ydeI"
                     /locus_tag="LAF92_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBI9791426.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            18495..19013
                     /locus_tag="LAF92_002433"
     CDS             18495..19013
                     /locus_tag="LAF92_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBI9791427.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
     gene            complement(19058..21103)
                     /gene="dcp"
                     /locus_tag="LAF92_002434"
     CDS             complement(19058..21103)
                     /gene="dcp"
                     /locus_tag="LAF92_002434"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBI9791428.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREALLSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            21240..21986
                     /gene="ydfG"
                     /locus_tag="LAF92_002435"
     CDS             21240..21986
                     /gene="ydfG"
                     /locus_tag="LAF92_002435"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBI9791429.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            22075..22761
                     /gene="ydfH"
                     /locus_tag="LAF92_002436"
     CDS             22075..22761
                     /gene="ydfH"
                     /locus_tag="LAF92_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBI9791430.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            22939..23142
                     /gene="ydfZ"
                     /locus_tag="LAF92_002437"
     CDS             22939..23142
                     /gene="ydfZ"
                     /locus_tag="LAF92_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBI9791431.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(23178..24638)
                     /locus_tag="LAF92_002438"
     CDS             complement(23178..24638)
                     /locus_tag="LAF92_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBI9791432.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDEKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(24727..26010)
                     /gene="ydfJ"
                     /locus_tag="LAF92_002439"
                     /pseudo
     CDS             complement(24727..26010)
                     /gene="ydfJ"
                     /locus_tag="LAF92_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            26727..26924
                     /locus_tag="LAF92_002440"
     CDS             26727..26924
                     /locus_tag="LAF92_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791433.1"
                     /translation="MDGESFNFDDNDIEFIASMYASCKLSGSIAPIMHIMVSIPKNKK
                     KQFYERVEHYCSLYSNKNPPS"
     gene            complement(27744..>28195)
                     /locus_tag="LAF92_002441"
     CDS             complement(27744..>28195)
                     /locus_tag="LAF92_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI9791434.1"
                     /translation="IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFIS
                     ELGPLPENVTWLSPGGEYQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAP
                     LQDAVDLEIATEEEISLLEAWKKYRVLLNRVNTTTAPDIEWPVAPIG"
BASE COUNT         7686 a         6910 c         6053 g         7546 t
ORIGIN      
        1 accactggtc gccatcagcg cgttaatttg ccgcgcctgc attggcgtca ccacaggttc
       61 atcaatatga atagctttcc cctgattagc cagaaagaca aacgtcctgg ccagctctac
      121 acagctcatt ttcagagcgc agtaatgaaa gtagttttgc agaacggttg tcacatcatg
      181 atggaaattg ccaaacgact tcatcagcca ggcgatagcc gcatttcgcg cggaatgttc
      241 aaattcggaa cgcgctacca ccgtatcgta tgaaatatca gacacaccgc ttaagccgcg
      301 cacgacttcc agcatacgtt gccgtggtgc gcttaatcgc ccttgcaaca tatcgcaaac
      361 caccagcgca ccagcattaa taaacggatt acgcggaata ccctgctcca tttccagttg
      421 cactaaagaa ttgaacggtg atccagacgg atctttgccg acgcgttgcc agatttcctc
      481 ttcggagtaa tgacgcatgg cgacaacgag actcagcact ttggaaatag actgaatgga
      541 aaaacgttct tgcgcgtctc cggcctgaaa aagctgtccg tcaacggtac agatagcaat
      601 gcccaatcgg gaaccgtcta ctgtagccag cgccggaata taatccgcga ctttaccctg
      661 accaatgagc ggccgcactt gccgcaagat gttttctaaa attgcattat ccatggcgac
      721 tgccactttc tactcctgga ccgcaggtct gaaaagacct gcgagtatat cagagctgaa
      781 tatgtcgcgt cagatccggt ctttccacac cgtctggata ttacagaatt cgtgtaagcc
      841 gaaatgggaa agctcacgac caaagccact ctttttcacg ccaccaaagg ccactcgcgc
      901 gtcgctggca caataaccat tgataaacac cccaccgcat tccagacgtg ccgccatctg
      961 tctggcctgt gtttcgtcgg tggtaaaaat ggtcgctgaa aggccgaact cactatcatt
     1021 agccagttcc agcgcatgtt ctgcatcttt cgcaacggtg attgccgcaa cagggccaaa
     1081 catttcttcc cgaaacgcgg tcatttctgg ggtaacattc gccagaaccg ttggcggata
     1141 gtaattacct gccccagcca tcttttcccc gcccagtaac aaacgcgcac cctgcgccag
     1201 ggttttctcc acctgatgat gcagctcatc acgtaaatca aaacgagcca ttggtccgag
     1261 agcgttctct tcatcacggg gatcgcccat tttcaaggct gccgcagctg ccacaaaacg
     1321 ttcggtaaat gcagaagcaa ttccctcttc gataataaag cgttttgccg ctgcacaaac
     1381 ctgtccggta ttctgataac gtccggctac cgccgcttta accgccagtt ccagatcggc
     1441 atcgttaagc acaataaatg gatccgaacc gcccagttcc agtacgcatt ttttcagtgc
     1501 cgctccagcc tgtgcgccaa tagccgctcc cgcacgaaca cttccggtca ccgtgacagc
     1561 agcaatgcgc gaatctttaa tcatttgact gacaccgtcg ttgtcggcat tcagccagcc
     1621 atatacgcct tgcgggattc ccgcatcttt aaacacctgg gcaatgagct gagcacagcc
     1681 catcacattc ggcgcatgtt taagtaagta gccgttacct gcaagaatga tgggaaccgc
     1741 gccacgcatc acctgccata acggaaagtt ccacggcata atcgccagaa tcgtccccaa
     1801 cggtcgatac tcaataactg cctgctgatt ttccaccagc gtaggttccg ccttcagcat
     1861 tgccggacca tgttctgcat accagtcaca caaattcgcc gatttcgcca cttcagcgcg
     1921 cgcctgattg attggtttgc ccatttcacg ggtgatcatt tgcgccattt cttcgctacg
     1981 ggcgcgcaga gccttaccga taccacgcag tttttcagca cgataatcta tatttgtctc
     2041 gcgccagtcg cgaaagcctg ctgccgccag ctgaagtgcg ttttcgatat cgttagcgcc
     2101 agcccacggc agcacagaaa gttgttcacc cgtggcagga tttattgaaa ttgcatgagt
     2161 tgccggagta atggtcatcg gggtatctcc tttatgagtc atggtatgaa gatacgcaga
     2221 tttactcttg ctttaaaatg aataatatta agccacttat tcacgaatcg agaatattat
     2281 ggatctgaca caactggaga tgttcaacgc ggttgccgaa gctggcagca taacccaggc
     2341 agcagcaata gtgcatcgcg tgccgtcgaa tctcactacc cgtctacgcc agctggaaac
     2401 agaactgggg gtagatctgt ttattcgcga gaatcagcgt ttacgtctct ctcctgccgg
     2461 gcataacttt ttacgctata gccaacaaat tctcgcgtta gtggatgaag cgcggagcgt
     2521 tgtcgctggc gatgagccgc aaggtttatt ttctcttggt tcgctggaaa gcaccgcagc
     2581 agtgcgcatt ccggccacgc tggcggaatt caaccatcgt tatcccaaaa ttcagttttc
     2641 actttccacc ggcccttccg gcactatgct ggaaggagtg ctggagggaa aactgaatgc
     2701 ggcatttatt gatgggccca ttaaccatac tgccattgac gggataccgg tataccgcga
     2761 ggagctgatg atcgtcacgc cacaaggtca tgcgccagta atccgcgcca gtcaggttaa
     2821 cggcagtaac atttatgcct tccgcgccaa ttgttcgtat cgtcgccact tcgagagctg
     2881 gtttcatgct gacggtgccg ctccgggaac tattcacgag atggagtcgt atcacgggat
     2941 gttggcctgt gtggtcgcag gagcaggcat tgcgcttatt ccacgctcta tgctggaaag
     3001 tatgccgggg catcaccagg ttgaagcgtg gccgttagct gagcaatggc gttggttaac
     3061 aacctggctg gtctggcgtc gcggtgcgaa aacgcgtccg cttgaggcat ttattcaact
     3121 actggatgtg cctgactcgg caaaacaggg ttaccaatga gctatttttg atagttcttg
     3181 cagctcaaat gccctatata gtgatgttcc gatgacttat gactatatgg ggcaaatatg
     3241 gttacgccag taagcatcag taattacata tctcttcctg atgattttcc cgctcgtaat
     3301 attgcacctc aggtaaaaga agttttaaag gactttattg atgcgctaag tacaataata
     3361 tgtgatgaag agtggcgcac gtctttaaac atcaactcag ccacaaaaaa gatatttaat
     3421 aaccttgata acttatcata tattcagagg acttcttttc ggggtaacga cacgctatac
     3481 aatgaaaagg ttcagtttaa actcacttat cccgtcaaga atggaagaca caaagaaaat
     3541 attgaatttc aggtagtaat aaatttaagc cctatttatt tagataattt tcgccatgat
     3601 ggagaaatta atatttttgc gctcccaacc caaagcctgt cactatgggg cgcgtatttc
     3661 agaccggcgt cgagcgtgtg ctatttctgt ttctgaatga ttttattgaa caatttccga
     3721 tgatcaaccc tggcgctccc atcaaaagag cgcatacacc acatattgaa cccctgcctc
     3781 cggatcacca taccgctgca gattacttac gccagtttga tttgcttgtc ctgaatttta
     3841 tctctcgcgg taattttgtc atactccccc gattatggaa taactctgag gttcacagat
     3901 ggttcgtcaa taaagatcct aacttgaaca ccgccattct cgacataacg gacagcgaat
     3961 taaaagagga tttattgcaa agcctgatgg attcattagg ttctaacaaa catgtactac
     4021 ccgaagtctg catctgcttt ttatcccttt tagcagaaca agaatctcct cattttcagg
     4081 acttgttttt atttttcgcc aatatgttat tgcactatca ccaatttatg aatcccaatg
     4141 aaagtgattt gaatgacgtg ttgatgccag catcattaag tgatgataaa attatcaaac
     4201 atatggcgcg caggaccctc aaactttttg taaaaaatga aacgccgcca aaagttactc
     4261 acgaagacct ggtgaaaaac aggcctcgct cccctgtcag accacctata cccgcaacct
     4321 ccaaaacgcc agacctccct gaacgtcatt aaaccgtgat gttaccgact ctctgacgcg
     4381 tgaaagaatc agcgtcagag aaacggaaaa cgcgatccag atcacaaatg cattgtattc
     4441 acatcattaa ccgttttaag atcatttcat cactttttcg caactcaccc gataatctgt
     4501 tatgacaaca aacactgttt cccgcaaagt ggcgtggcta cgggtcgtta cgctggcagt
     4561 cgccgccttc atcttcaaca ccaccgaatt tgtccctgtt ggcctgctct ctgacattgc
     4621 gcaaagtttt cacatgcaaa ccgctcaggt cggcatcatg ttgaccattt acgcatgggt
     4681 agtagcgcta atgtcattgc cttttatgtt aatgaccagt caggttgaac ggcgcaaatt
     4741 actgatctgc ctgtttgtgg tgtttattgc cagccacgta ctgtcgtttt tgtcgtggag
     4801 ctttaccgtt ctggtgatca gtcgcattgg tgtggctttt gcacatgcga ttttctggtc
     4861 gattacggcg tctctggcga tccgtatggc tccggccggg aagcgagcac aggcattgag
     4921 tttaattgcc accggtacag cactggcgat ggtcttaggt ttacctctcg ggcgcattgt
     4981 gggccagtat ttcggttggc gaatgacctt cttcgcgatt ggtattgggg cgcttatcac
     5041 ccttttgtgc ctgattaagt tacttccctt actgcccagt gggcattccg gttcactgaa
     5101 aagcctcccg ctattgttcc gccgcccggc attgatgagc atttatttgt taactgtggt
     5161 ggttgtcacc gcccattaca cggcatacag ctatatcgag ccttttgtac aaaacattgc
     5221 gggattcagc gccaactttg ccacggcatt actgttatta ctcggtggtg cgggcattat
     5281 tggcagcgtg attttcggta aactgggtaa tcagtatgcg tctgcgttgg tgagtacggc
     5341 gattgcgctg ttgctggtgt gcctggcatt gctgttacct gcggcgaaca gtgaaataca
     5401 cctcggggtg ctgagtattt tctgggggat cgcgatgatg atcatcgggc ttggtatgca
     5461 ggttaaagtg ctggcgctgg caccagatgc taccgacgtc gcgatggcgc tattctccgg
     5521 gatatttaat attggaatcg gggcgggtgc gttggtaggt aatcaggtga gtttgcactg
     5581 gtcaatgtcg atgattggtt atgtgggcgc ggtgcctgct tttgccgcgt taatttggtc
     5641 aatcattata tttcgccgct ggccagtgac actcgaagaa cagacgcaat agttgaaagg
     5701 cccattcggg ccttttttaa tggtacgttt taatgatttc caggatgccg ttaataataa
     5761 actgcacacc catacatacc agcaggaatc ccatcagacg ggagatcgct tcaatgccac
     5821 ccttgcccac cagccgcata attgcgccgg agctgcgtag gcttccccac aaaataaccg
     5881 ccaccaggaa aaagatcagc ggcggcgcaa ccatcagtac ccaatcagcg aaggttgaac
     5941 tctgacgcac tgtggacgcc gagctaataa tcatcgctat ggttcccgga ccagcggtac
     6001 ttggcattgc cagcggcaca aaggcaatat tggcactggg ttcatcttcc agctcttccg
     6061 acttgctttt cgcctccggt gaatcaatcg ctttctgttg cggaaagagc atccgaaaac
     6121 cgataaacgc gacgattaag ccgcctgcaa ttcgcagacc gggaatcgaa atgccaaatg
     6181 tatccatcac cagttgcccg gcgtaatacg ccaccatcat gatggcaaat acgtacaccg
     6241 aggccatcaa cgactgacga ttacgttcgg cactgttcat gttgcctgcc aggccaagaa
     6301 ataacgcgac agttgttaat gggttagcta acggcagtaa caccaccagc cccaggccaa
     6361 ttgctttaaa caaatctaac attggtggtt gttatcctgt gtatctgggt tatcagcgaa
     6421 aagtataagg ggtaaacaag gataaagtgt cactctttag ctaaccttgc atcgcattga
     6481 ccaaaacttg aaccgattta gcaaaacgtg gcatcggtca attcatttat ttgacttata
     6541 cttgcctggg caatattatc ccctgcaact aattacttgc cagggcaact aatgtgaaaa
     6601 gtaccagcga tctgttcaat gaaattattc cattgggtcg cttaatccat atggttaatc
     6661 agaagaaaga tcgtctgctt aacgagtatc tgtctccgct ggatattacc gcggcacagt
     6721 ttaaggtgct ctgctctatc cgctgcgcgg cgtgtattac tccggttgaa ctgaaaaaag
     6781 tgttgtctgt cgaccttgga gcactgaccc gtatgctgga tcgcctggtc tgtaaaggct
     6841 gggttgaaag gttgccgaac ccgaatgata agcgcggcgt actggtaaaa cttaccacca
     6901 gcggcgcggc aatatgcgaa caatgccatc aattagttgg ccaggacctg catcaagaat
     6961 taacaaaaaa cctgacggcg gacgaagtgg caacacttga gcatttgctt aagaaagtcc
     7021 tgccgtaaac aaaaaagagg tatgacgatg tccagacgca atactgacgc tattaccatt
     7081 catagcattt tggactggat cgaggacaac ctggaatcgc cactgtcact ggagaaagtg
     7141 tcagagcgtt cgggttactc taaatggcac ctgcaacgga tgtttaaaaa agaaaccggt
     7201 cattcattag gccaatacat ccgcagccgt aagatgacgg aaatcgcgca aaagctgaag
     7261 gaaagtaacg agccgatact ctatctggca gaacgatatg gcttcgagtc gcaacaaact
     7321 ctgacccgaa ccttcaaaaa ttactttgat gttccgccgc ataaataccg gatgaccaat
     7381 atgcagggcg aatcgcgctt tttacatcca ttaaatcatt acaacagcta gttgataacg
     7441 tgacaacgtc actgaggcaa tcatgaaacc actttcatcc gcaatagcag ctgcgcttat
     7501 tctcttttcc gcgcagggcg ttgcggaaca aaccacgcag ccagttgtta cttcttgtgc
     7561 caatgtcgtg gttgttcccc catcgcagga acaaccaccg tttgatttaa atcacatggg
     7621 tactggcagt gataagtcgg atgcgctcgg cgtgccctat tataatcaac acgctatgta
     7681 gttattgtca ggctctgatc acagagcctg acatctccgt aatatcttcc tgaagtctca
     7741 aatattttcg ccattgttgg cgatgagatc ccgataccag taaaacgacg ctttacgtat
     7801 ccgttccagc gttcccttcc cttcgttgtc tttatcgaca taaatcattc cgtagcgctt
     7861 tttcatttcc cctgttccgg cagaaaccag gtcaatgcag ccccacgggg tgtagccaat
     7921 taagtcaaca ccatcttcaa ctacggcttt tttcatttcc cgaatatggg aagcaaagta
     7981 atcaatgcga tagtgatcgt tcaccgtgcc gtcagcttgt ctctggtcaa ccgcaccaaa
     8041 tccattttcg acaataaaca gcggcaactg gaaatgatcc cagaaccagt tgagtgaata
     8101 acgtagccct gccggatcaa tttgccatcc ccattcggat gtatcgatat aagggttgct
     8161 gaccaaatct cgtggttcaa cataatccag ttgcgggtta tcgggcgaaa attgtgtcgt
     8221 aaaagacatg tagtagctaa agccgataaa gtcgacacaa ccactggcaa gaatcgcgtt
     8281 atcttctggt gtgatatcga ggttgaatcc tttcctggca aagtaattca gcatatgttg
     8341 cggataatat ccacgagcat gaacatcagt aaaccagtaa cgacgatgca ttgctttcgt
     8401 ggccatcatc atatcgttgg gtgcacacgt cagaggatag atgggacaca tggcgatcat
     8461 acagccaatg ttaaattcag gattgatcga ttttccagtc tgtaccgcca gggcactggc
     8521 aactaactcg taatgtaccg cctggtacat tatttgttcg cgctcattga tatcttcctc
     8581 tggcgaatac aagataccgg aattagtaaa tggacacagg ctttcgctga aattcacctg
     8641 attattgatt tcgttaaacg tcatccagta ctttactttt tctttatagc gcgtgaagac
     8701 cgttgatgcg aagcggataa aaaagtcgat cagtttacgg tttcgccagc caccatattt
     8761 tgtcaccaga tgataaggca tctcaaaatg tgaaagcgtc accacaggtt ccattccctg
     8821 cttaaggcat tcatcgaaca gatcatcata aaattgtaaa ccctcttcat tcggctcctg
     8881 ttcgtcacct tgcggaaaga ttcgtgtcca ggcaatggaa gttcgaaagc atttgaatcc
     8941 catctcggca aataactgaa tatctgtttt atagcgatga taaaaatcaa ttgcttcatg
     9001 attgggataa ttaagcccgt cgataacgcc ttctgtcact tcacgcggca ccccgtgagc
     9061 gccagcagtc atcacatcag cgatgctgat gccttttcct ccttcattcc agccaccttc
     9121 caactgatgc gcggctaccg caccgcccca taaaaaacct tttttaaatc ctgacataca
     9181 caactcctta actaaaatag attaccacca gacttccgcc tggatgccgg ccataaactc
     9241 gtctttgctg ttctcattaa acttaaaatt cgataattcg ttatccagaa tattgatata
     9301 cgttccatag aagcgaattt ctggtcgtga acctaaaatg ctttcaccca ctttcaatgc
     9361 atgccagagt gtcgttttat aagccgattc atttagtgtt acaccctgct gagttttgtt
     9421 ctgttgctta aaccagccta attcaagccc cgtctgattc catgtattcc agatccaggc
     9481 cggtcttatt acagtgcgaa tactgtcaaa atcactatga gcaccacttt cataactata
     9541 aacatcttcg ccgtgagaat agacaagcgc gttagccata ataatattgt cagtaagata
     9601 catctcgccc tgagagatta aacgccaggc gatcccattg gtatgatcac catagtaata
     9661 gcgaccatgc gccatcgtat tactggcatc tgagaaactg gcaaaactgc tggcatagga
     9721 attattggca acctgtaacg taaattcatt aaacgtatcg cgttgcaggt tttgccgtaa
     9781 aacagaagtc agcatccagc tatctttcat ttcaaaataa ctgttgtcat tttcgttatc
     9841 ttgttgttga tccgttttat taggtgcgga atatttagcc attaatgata atgtcgcatc
     9901 atcccataac gggatattgc gataacgaac atccacagaa ttggtattca tctggctggt
     9961 acgcgaaaaa tcacgagagt aaacatcgac atcatcacgg cttaaggaca tatcaaacag
    10021 ccctacacca agtgcccagt tttcaatccc cacacccgcg gcgacatccg tggttaaggt
    10081 tttccagtcc agcatctgga tctcatattg cgggagttta tgtttgccga cccagaagtc
    10141 tgcctctggt gcgaagggta aaaaacctcg cgttgtcagg taaatatcgc tgaactgcat
    10201 gatattttca ttggcactgt caccaaacca ggcatcgtta tactgctcac ccacgttgcc
    10261 atcataggta acgaccgcat ttgccgtttt accgtcctga ttataaacac gctgatttaa
    10321 ggtcaggtca aaccaaccgc tcatctcatt accaaaacgt cccagagaac ccgctgcata
    10381 agtttgtggt gagccgtgat taccagtacc ccagcctgag cggaagtacc cctgatagct
    10441 gaacccaata tcatccttta tatatttact gatatctttc agggtcacgc tttcgatagt
    10501 ggttttgtcg cctttacttt caataacggc aacttgctgc gatccattta tagtatgcgc
    10561 tgcggcagac gagttctgtt caccgttttc atttttgatg ttatcagcga cgggagatgt
    10621 cgctgatatt tcagcaagcg aggctgattt atatttgttt tccgtgatgc ttttttgtaa
    10681 ggtcgaaagt cgggatttat atactcccag ttcattctgt gttgctttca gctcttgttt
    10741 attttgcgac aattcatttt caagcaattc aaggcgctgc tctatggtta attttgttgc
    10801 catcgcgttc aatggaaaca atgctgtaat aatagaaaaa gacaaaaggc tgacgttaag
    10861 cgtctttata ttcatgactt atccttaaga gtataataat ttcctccagc tatcgcactg
    10921 gaggaaaaaa tcaggcatgg agttcttttc ttaatgaaat aatttccctg gctaaatcct
    10981 ggcataatat tgcattcatc aaatgatcct gtgcatgaat cagaattaaa ttaactttta
    11041 ttttgccaca gccttcatcc tgactaatta atgcggtctg aatcttatgt gcctgaagac
    11101 aggcagattc agaactggcc attagctgag ttgcgccctg ccagtctttt tgcccggcac
    11161 aacgtatggc ttccatcgcg tcagaacgtg cctgccccgc gttgaccagt aactccataa
    11221 caagttcttc atctgcaaac atcacaatct ccttcctggg taaatggaat tatccgttag
    11281 ttgtcatgtt attgactttg ttggcggcaa taacaaaagg taaatagatc attgtcgcca
    11341 cacctaaaca taaaaaacca accagtaaag ccagccagtt ccccccggtt ccgaggaaag
    11401 caattaaagg tccgggcgta gtccagggca cggcataagc aaccggtgga ataatttcca
    11461 tcgaaacaaa gaagtaacca atggcacagt taacaatggg aaccataata aacggcacca
    11521 tcatgatggg gttaagtaca ataggaaggc caaacattat cggttcattg atattgaaaa
    11581 taccggggat aaatgacatt tttgccagat cacggtattc cgcacgctta gaagcgataa
    11641 aaatagccaa caataacccc aacgtcatac ctgaaccgcc gcagttggcg aatgcatcat
    11701 taatagaagt ccaggtaatc ggatatggcg cgccccaggt agtgccatgt tgagcggccc
    11761 aggagagatt ctccagatta gcctcagaaa aaacggtttc tcgaattgcc gatgtggtat
    11821 taggtccgtg gatccccagc acccacagga aattacccac agcccccagg ataattaccg
    11881 caaagatatt cgttcccata tgtttgagcg gcgtctggat taatgtgtaa ataagatcgt
    11941 ttagtcctgc cggggatatc agtgtcaggc aataattaag tgcggaaaag aacaccatga
    12001 caaaaaatat tggcaataaa actttaaatg aacgcgctac cgcaggcggt acagctgccg
    12061 gcatcgttat ggtgattttt gggtttcgag ctaagcgaca aaatatttca ctggtgatca
    12121 atgcaacgat aacagcaaca aacaacccct gcgcgccgag atatttggtc gtcaggaatg
    12181 cttgcccatc tatcatctga tatggcgtat aaacaataaa aaatgctcct attgccgtta
    12241 atccgcataa aagatcatct tgctcatagc taatcgccat attcctggcg accaggaaag
    12301 caatcataat tgacatgata tttacagaac cattcattac cggagtaaaa atagcttgtg
    12361 ctttttcaag gttggggaaa atgctattca gatgcagtaa tccggcaata aaaccgtctg
    12421 gcgataatat ggcaaagtta attaatataa ttaatgaact tgccatgata attggaaacg
    12481 taaaaacgaa tccatctctc accgctgcaa catgtttttg tgagtttaac ttgatagcaa
    12541 ctggaacaag aaaacgttcc attccacgtt cgaatgaggc cattaatccc atttttttat
    12601 acctgttgtt ttggatattt atacatccca ataaagcatg tatgccatat ccattttatg
    12661 atgtgtgaaa agttagttca ggtaatagtt taataatatt agattcttac tcttgcagta
    12721 gtttcatcgc aaattcaaga actgcctgtc cgttcatggt gccgtagtct ttcatctcga
    12781 taacggctac aggcattttc ccctgtgcaa cggcttcaat ctctggtttc tgaaagcgaa
    12841 cttgcgggcc aagtaaaaca acgtcaactt cgttattttt aattttccct ttagcctctg
    12901 caatcgccaa tgcggaaata ttaacttcaa gtgaaatagc gttagcatga tcaatcatac
    12961 gtttaaccag catactggtt gacatgcccg cagcgcaaac taacaatatt tttttcatat
    13021 tttcccttac tggtatataa cagattacat tgtttaaata tcatatcccg cagtagctat
    13081 acgtcctggt acattgatca cagtaataca gtgataaagt tgttcaacat cagggtgatt
    13141 attaaacgac tcggccatac gataaaccaa tcaggactca actccttgtt ttcaatggct
    13201 cttaaaaggt gcaaaagtga tttttcaaaa gattgcccgc ttacttaaat ccgaaatcaa
    13261 tggcaattca tggcatgttg gtgatttgtt gccgtcagaa gcggaactcg ctgttcgtta
    13321 taatgtttcg cgtaataccc tgcgtaaggc attatccttg ctggaaggcg aaggaattat
    13381 tcacagaaag catggttcag gaacatacat tcaaaaaaag aattttgttg cacacattga
    13441 tcacatgaac agtttcagtg aaattgcgca taaaagcggc aaagaggcag ggagtcagat
    13501 tatgaaattt gaagtgcagg atgcttcacc cactattgcg actgagctga atttagtgac
    13561 tggtgagcag gtttattaca taaagagact gcgattcatt gaggataatg cagcacaatt
    13621 agaagaaacg tggatgtcag tagcacgttt tcctgattta accgtgtcac atatgcaaaa
    13681 atccaaattt tcatatattg agaatgaatg cgggatcaaa atcattggca cctttgaaac
    13741 tttctcccct acttttccta ccccagaaat cgccagtatt ttacggatca gcccacggga
    13801 tcccatactt aaaattcaga cccaggctgt ggacagtaac tctattccgc tggattattc
    13861 gttactttac agcaatattt tcgagttcca ggtaaagtac tttttcccgc gataaaagca
    13921 gacgcacaga gtgctgacat cacattctgt gcgtatggtt tagcctctaa cctttaccgc
    13981 tttacgccac cgcaagccaa atacattgat atacagcccg gtcataatga gcaccgcacc
    14041 taaaaattgc agacccgtta agcgttcatc caacaatagt gccgcacttg ccagtccgac
    14101 tacgggcacc agtaacgata acggtgcaac ccgccaggtt tcatagcgtc ccagtaacgt
    14161 cccccagatc ccataaccaa caattgtcgc cacaaacgcc agatacatca gcgacaagat
    14221 ggtggtcata tcaatagtaa ccagactgtg aatcatggat gcggaaccat cgagaatcag
    14281 cgaggcaaca aagaagggaa tgattgggat taaagcgctc cagattacca gcgacatcac
    14341 cgccggacgc gttgagtgcg acatgatctt tttattgaag atgttgccac acgcccaact
    14401 aaatgctgca gccagggtca acataaagcc gagcatcgcc acatgctgac cgttcagact
    14461 atcttcgatt aacaccagta cgccaaaaat cgctaaggcg atccccgcca attgtttgcc
    14521 atgcaatcgc tccccgaaag taaacgcgcc aagcacgata gtaaaaaacg cctgtgcctg
    14581 taacaccagc gaagccagtc cagcaggcat accgaagtta atggcacaaa aaagaaaagc
    14641 aaactgcgca aaactgatgg ttaatccata ccccagcagc aaattcagtg gtactttcgg
    14701 tcgtgcgaca aaaaagatag ccggaaaagc gaccagcata aagcgcaaac cggccagcat
    14761 cagcggtggc atgttatgaa gccccacttt gatgaccaca aaatttagcc cccatacgac
    14821 cactaccagt agcgccaaca ccccatcttt tcgcgacatt ctaccgcctc tgaatttcat
    14881 cttttgtaag caatcaactt agctgaattt acttttcttt aacagttgat tcgttagtcg
    14941 ccggttacga cggcattaat gcgcaaataa gtcgctatac ttcggatttt tgccatgcta
    15001 tttctttaca tctctaaaac aaaacataac gaaacgcact gccggacaga caaatgaact
    15061 tatccctacg acgctctacc agcgcccttc ttgcctcgtc gttgttatta accatcggac
    15121 gcggcgctac gctgccattt atgaccattt acttgagtcg ccagtacagc ctgagtgtcg
    15181 atctaatcgg ttatgcgatg acaattgcgc tcactattgg cgtcgttttt agcctcggtt
    15241 ttggtatcct ggcggataag ttcgacaaga aacgctatat gttactggca attaccgcct
    15301 tcgccagcgg ttttattgcc attcctttag tgaataacgt aacgctggtg gtgctctttt
    15361 ttgccctcat taactgcgcc tattctgttt ttgctaccgt gctgaaagcc tggtttgccg
    15421 acaatctttc gtccaccagc aaaacgaaaa tcttctcaat caactacacc atgctaaaca
    15481 ttggctggac catcggtccg ccgctcggca cgctgttggt gatgcagagc atcaatctgc
    15541 ccttctggct ggcagctatc tgttccgcgt ttcccatgct tttcattcaa atttgggtaa
    15601 agcgcagcga gaaaatcatc gccacggaaa caggcagtgt ctggtcgccg aaagttttat
    15661 tacaagataa agcactgttg tggtttacct gctctggttt tctggcttct tttgtaagcg
    15721 gcgcatttgc ttcatgcatt tcacaatatg tgatggtgat tgctgatggg gattttgccg
    15781 aaaaggtggt cgcggttgtt cttccggtga atgctgccat ggtggttacg ttgcaatatt
    15841 ccgtgggccg ccgacttaac ccggctaaca tccgcgcgct gatgacagca ggcaccctat
    15901 gtttcgtcat cggtctggtc ggttttattt tttccggcaa cagcctgcta ttgtggggta
    15961 tgtcagctgc ggtatttact gtcggtgaaa tcatttatgc gccgggcgag tatatgttga
    16021 ttgaccatat tgcgccgcca ggaatgaaag ccagctattt ttccgcccag tctttaggct
    16081 ggcttggtgc cgcgattaac ccattagtga gtggcgtagt gctaaccagc ctgccgcctt
    16141 cctcgctgtt tgtcatctta gcgttggtga tcattgctgc gtgggtgctg atgttaaaag
    16201 ggattcgtgc aagaccgtgg gggcagcccg cgctttgttg atttaagtcg aacacaataa
    16261 aaatttaatt cagccttcgt ttaggttacc tctgctaata tctttctcat tgagatgaaa
    16321 attaaggtaa gcgaggaaac acaccacacc ataaacggag gcaaataatg ctgggtaata
    16381 tgaatgtttt tatggccgta ctgggaataa ttttattttc tggttttctg gccgcgtatt
    16441 tcagccacaa atgggatgac taatgaacgg agataatccc tcacctaacc ggccccttgt
    16501 tacagttgtg tacaaggggc ctgattttta tgacggcgaa aaaaaaccgc cagtaaaccg
    16561 gcggtgaatg cttgcatgga tagatttgtg ttttgctttt acgctaacag gcattttcct
    16621 gcactgataa cggatcgttg acacagtagc atcagttttc tcaatgaatg ttaaacggag
    16681 cttaaactcg gttaatcaca ttttgttcgt caataaacat gcagcgattt cttccggttt
    16741 gcttaccctc atacattgcc cggtctgctc ttccaatgac cacatccaga ggctcttcag
    16801 gaaatgcgcg actcacacct gctgtcacgg taatgttgat atgcccttca gaatgtgtga
    16861 tggcatggtt atcgactaac tggcaaattc tgacacctgc acgacatgct tcttcatcat
    16921 tagccgcttt gacaataatg ataaattctt cgcccccgta gcgataaacc gtttcgtaat
    16981 cacgcgtcca actggctaag taagttgcca gggtgcgtaa tactacatcg ccgattaaat
    17041 gcccgtaggt atcattaacc aatttaaatc ggtcaatatc caacaacatt aaataaagat
    17101 tcagaggctc agtgttgcgt aactgatgat caaaggattc atcaagaacc cgacgccccg
    17161 gcaatcccgt caaaacatcc atattgctac ggatcgtcag caaataaatt ttgtaatcgg
    17221 ttaatgccgc agtaaaagaa agcaacccct cctgaaaggc gtcgaaatgc gcgtcctgcc
    17281 agtgattttc aacaatagcc agcattaatt cccgaccaca gttatgcata tgttgatggg
    17341 cagaatccat tagccgaacg taaggtaatt catcgttatc gagtggcccc agatgatcaa
    17401 tccaccgacc aaactggcac agtccataag aatggttatc cgttatttct ggcttactgg
    17461 catctctcgc gaccacgctg tgaaacatac tcaccagcca ctggtagtgg gcatcgatag
    17521 ccttattgag atttaacaag atggcatcaa tttccgttgt cttcttgatc attgccactc
    17581 ctttttcaca gttccttgtg cgcgctattc taacgagaga aaagcaaaat tacgtcaata
    17641 ttttcataga aatccgaagt taggagtcat ctctgagata acattgtgat ttaaaacaaa
    17701 atcagcggat aaaaaagtgt ttaattctgt aaatcacctc tgcattatcg taaataaaag
    17761 gatgacaaat agcataaccc aataccctaa tggcccagta gttcaggcca tcaggctaat
    17821 ttatttttat ttctgcaaat gagtgacccg aacgacggcc ggcgcgcttt tcttatccag
    17881 actgccgcta atgttgatca tctggtccgg ctgaacttct cgtccatcaa agacggccgc
    17941 aggaataacg acattaattt caccgctctt atcgcgaaaa acgtaacggt cctctccttt
    18001 gtgagaaatc aaattaccgc gtagtgaaac cgaagcgcca tcgtgcatgt tttttgcgaa
    18061 atcaacggtc attttttttg catcatcggt tccgcgatag ccatcttcta ttgcatgagg
    18121 cggcggtggc gctgcatcct gttttaaacc gccctggtca tctgccaacg cataaggcat
    18181 gacaagaaaa cttgctaata caatggcctg aaatttcata ctaactcctt aattgcgttt
    18241 ggtttgactt attaagtctg gttgctattt ttataattgc caaataagaa tactgccaat
    18301 tgttataagg catttaaaat cagcccacta gctgtcaaat attcagagaa tttaactcac
    18361 taaagttaag aagattgaag agtcttaaac atattttcag aataatcgga tttatatgtt
    18421 tgaaaattat tatatttgac gagcatacag aaaaagcaaa tcacctttac atataatagc
    18481 gtggacaaaa aacagtgaac attaataaag ataaaattgt acaactcgca gataccgaca
    18541 ctattgaaaa cctgacatcc gcgttgagtc aaagacttat cgcggatcaa ttacgcttaa
    18601 ctaccgccga atcatgcacc ggcggtaagt tggctagcgc cctgtgtgca gctgaagata
    18661 cacccaaatt ttacggtgca ggctttgtta ctttcaccga tcaggcaaag atgaaaatcc
    18721 tcagcgtaag ccagcaatct cttgaacgat attctgcggt gagtgagaaa gtggcagcag
    18781 aaatggcaac aggtgccata gagcgtgcgg atgctgatgt cagtattgcc attaccggct
    18841 acggcggacc ggagggcggt gaagatggta cgccagcggg taccgtctgg ttcgcgtggc
    18901 atattaaagg ccagacctac actgcggtta tgcattttgc tggcgactgc gaaacggtat
    18961 tagctttagc ggtgaggttt gccctcgtcc agctgctgca attactgcta taaccaggct
    19021 ggcctggcga tatctccagg ccagccattg gtggtgttta tatgttcaag ccacgatgtt
    19081 gcagcatcgg cataatttga ggagccttac cgcgccattg tcgatacagg cgttccagat
    19141 cttcgctgtt acctctggaa aggagcgcct cgcgaaaacg ctgcccattt tcacgcgtta
    19201 atccgccctg ctcaacaaac cactggtaac catcatcggc caacatttgc gtccacagat
    19261 aagcgtaata acctgcagca tatccgccac caaaaatatg ggcgaaataa ctgctgcgat
    19321 agcgtggcgg tatagcagga agatccatat tttccgccac cagcgcccgc aattcaaaat
    19381 catcgacatc ctgcattgct tcgttttctt ccaggcaatg ccagcgcata tcgagaagtg
    19441 cggcgctaag cagttcgctc atctcatacc ctttgttgaa caggctggca ttacgcattt
    19501 tctgttgcag ttcgtcaggc attgctgccc cgctctgata atgccgggcg tagcgagcga
    19561 atacctgcgg atgcgttgcc cagtgttcgt tgatttgcga cggaaattcg acaaaatcac
    19621 gcggcgtgtt ggtgccggaa agcgtggcat aacgctggcg ggcaaaaagg ccgtgcagcg
    19681 tatgaccaaa ttcatggaat aaggttatga catcatccca gagtaacaac gcaggctcac
    19741 cggcagcggg tttctgataa ttgcaaacgt tataaattac cggatgcgtt tcattaagcg
    19801 ttgattgctc aacaaaattg cccatccatg caccgccgct ttttgaatca cgggcgaaga
    19861 aatcaccgta aaataacgcc agtcccacgc cattatgatc aaaaatttcc cacacacgaa
    19921 cgtcaggatg gtagacagga atatcaaaac gttcgacaaa cttaataccg aagagctgat
    19981 tcgcggtcca gaatacacct tcattcaaca ccgtgtttaa ttcaaaatat ggcttgagct
    20041 gcgcctcatc aagatcaaat ttctcccgcc gtacctgttc ggcataaaat gcccagtccc
    20101 acggctgcgc gctaaacccg ccctgctgct tatcaataac cgcctgtatg gaggctaatt
    20161 catcgctcgc acgttgacgc gccgctggaa caatttcccg cataaagttg agcgctgctt
    20221 caggtgtttt tgccatctga tcggcgattt tccatgcggc ataatgagga aaaccaagta
    20281 atgtcgcctg ttgtgcacgg atctccacca gacgttgaat gatagcgcgg gtatcattgc
    20341 catcattttt ttccgctcgc gtccagcccg caataaacag tttttcacgc gtcgcacgat
    20401 cgcgcatttc ggcaagcgcc ggctgttggg tggtattcag cagcggaatc agccatttgt
    20461 tatccagacc tttctcgcga gccgcctctg ccgccagcgc aatctcttgc tcactcattc
    20521 ctgccagctg cgcgatatcg ttcacaacca gaccgccgga tttatttgct gccagtaacc
    20581 gctggttaaa ctgactggtc agtgtcgcag cttctgtatt cagtactttt aattttgctt
    20641 tatcagcttg cgcaagtttg gctccggcaa ggacaaaacg ttgatgaatc acctccacca
    20701 ggcggatgga ttcactatca agccccaggg attcacggcg ctgccagaca gcatctaccc
    20761 gcgcgaataa ttcaccgttc agatagatat cattagccag ttccgccagt tcagcggaaa
    20821 actgttcatc aagacgctgt aattcatcat tggtatgcgc cgcagtcatc gcaaaaaaga
    20881 cgctggtaac gcgggtaagt aattctccgc tttgttccag tgccagaata gtattgttga
    20941 aatcaggcgt ttgcgggtta agcgcgatgg cagcaatttc tgcccgcttt tgctgcattc
    21001 cctcatcgaa tgccgggcga tagtgatgat tggcaatttg atcaaaatga ggagccagat
    21061 acggcagtgt gctttgcaca aggaaaggat tcattgttgt cattttcttc tcctgaacgc
    21121 gaggtgttcc atagcgtagg cttactgata acggagtgca atcttgcaat ccagtattac
    21181 ccgctcttaa gcatcccgtg ctatgttatt gacacacaaa agcgttgagg aacagtgaga
    21241 tgatcgtttt agtaactgga gcaacggcag gttttggtga atgcattact cgtcgtttta
    21301 ttcaacaagg gcataaagtt atcgccactg gccgtcgtca ggaacggttg caggagttaa
    21361 aagacgaact gggagataat ctgtatatcg cccaactgga cgttcgcaac cgcgccgcta
    21421 ttgaagagat gctggcatcg ctccctgccg agtggagcaa tattgatatc ctggtaaata
    21481 atgccggcct ggcgttgggc atggagcctg cgcataaagc cagcgttgaa gactgggaaa
    21541 cgatgattga taccaacaat aaaggcctgg tatatatgac gcgcgccgtc ttaccgggta
    21601 tggttgaacg taatcatggt catattatta acattggctc aacggcaggt agctggcctt
    21661 atgccggtgg taacgtttac ggtgcgacga aagcgtttgt tcgtcagttt agcctgaatc
    21721 tgcgtacgga cctgcatggt acggcggtgc gcgtcaccga catcgaaccg ggtctggtgg
    21781 gtggcaccga gttttccaat gtccgcttta aaggcgatga cggtaaagcg gaaaaaacct
    21841 atcaaaatac cgttgcattg acgccagaag atgtcagcga agccgtctgg tgggtgtcaa
    21901 cgctgcctgc tcacgtcaat atcaataccc tggaaatgat gccggttacc caaagctatg
    21961 ccggactgaa tgtccaccgt cagtaatttt tatacccggc gtaactgccg ggttattgct
    22021 tgtcacaaaa aagtggtaga ctcatgcagt taactcactc acaagcaaga acgaatgacc
    22081 gtcgaaacgc aacttaatcc cacacagcct gtcaatcagc agatttatcg tattcttcgt
    22141 cgcgacattg tccattgcct gattgcacca ggcacaccgt tgtcggaaaa agaagtttct
    22201 gttcgtttca atgtgtcacg ccagccggtt cgtgaagcct ttattaaact ggcggaaaac
    22261 ggcctgattc aaattcgtcc gcagcgtggc agctacgtca acaaaatttc catggcccag
    22321 gtgcgaaacg gcagttttat ccgtcaggcc attgagtgcg cggtggcgcg tcgggcggcg
    22381 agcatgatta ccgaaagcca gtgctaccaa ctggaacaaa atcttcacca gcaacgcatt
    22441 gccattgagc gcaagcaact ggatgatttt tttgaacttg atgacaactt ccatcaactc
    22501 ctgacgcaga ttgccgactg tcaactggcg tgggatacca ttgagaacct gaaagcgacc
    22561 gttgatcgcg tgcgctatat gagtttcgac catgtttctc caccagaaat gctgttacgc
    22621 cagcatcttg atattttctc tgccctgcaa aaacgtgatg gcgatgcggt agaacgtgca
    22681 atgacgcaac atttgcagga aatcagcgaa tccgtgcgcc agatccgcca ggaaaacagc
    22741 gactggttta gcgaagagta attcatttac tctcatccca tccgggatga gaggcttttc
    22801 cccccgcctt acggctcatg catgcatcaa aaaagatgtg agcttgatca aaaacaaaaa
    22861 atatttcact cgacaggagt atttatattg cgcccgttac gtgggcttcg actgtaaatc
    22921 agaaaggaga aaacacctat gacgacctac gatcgtaacc gtaacgcaat caccactggc
    22981 agccgtgtta tggttagcgg caccggtcac actggcaaga tcctgtcgat tgatactgaa
    23041 ggtctgaccg ctgagcaaat ccgccgcgga aaaaccgtag ttgttgaagg ttgtgaagag
    23101 aaactggcac ctctggacct gattcgtctc ggcatgaact aagtgtgtga atgccgccga
    23161 tggcggcatt gcttttttta cttcacggaa tatttcgcca cggtcgcttt cgcgccatgc
    23221 gctaataaag acaagtacgc ttccgtcact tttgcagtaa acaagctatt gtctggcaaa
    23281 tcatcaccaa agattgcctt aatcgccagc aatgactgga cgcgcgcttt cccttcggca
    23341 ctactttgta cagccttctg aataacaggt aacagtgggt cactgatttc tatcggattt
    23401 ccctgttcat caacaccacc gacataacgc atccaacccg cgacgcccag cgccagcaga
    23461 tcgaacttgc tgtcatgcgc cagatgccag cgaacagaat ccaacatccg ctgtggcaat
    23521 ttctggctac catccatcgc aatctgccag gttcgatgac gtaacgccgg gttgctatag
    23581 cgtgcaatta atcggttagc gtaatcttgc aaatcaacgc cctgcacttt cagcgtcggc
    23641 gcttgttcct gcaacatcaa gccatacgcc gcataacgat aatgttcatc ttccatgcag
    23701 tcattaatgt gctgatatcc agcaagataa cccaaatacg ccaggaatga atgactgccg
    23761 ttgagcatgc gcaacttcat ctcttcataa ggcagcacat cactaaccag ttcggctccc
    23821 gctttttccc attctggacg tccggcaaca aagttatctt ctattaccca ctggcggaaa
    23881 ggttcacagg caacgcccgc aggatcgccc acaccggtaa gttgttcgat tttcgccagc
    23941 gtatcctctg tcactgcggg cacaatacgg tccaccattg ttgatgggaa agtcacgtta
    24001 tcttcgatcc attgtgccag tttttcatca acggcttgcg cgtaggaagt gacaacgtca
    24061 cgcataacat gaccgttttc tggcatgttg tcacatgaca tgacggtaaa tgcgggaagt
    24121 cctgccgctt tacggcgagc cagcgcctca acaatcactc ctgttgctgt tttcggctgg
    24181 tggggatttt gcacgtcggc agcgaccatc gggtgatcga gcattaactg tccggtcgcc
    24241 ggagagtgga aatacccttt ttcggtgatt gtcagagaga caatcgcgat ttgcggttca
    24301 cacatcgcag ccaacacggt ttctaagcca tccatctgta cgtgcaaggc ttttttaacg
    24361 acgccaacga cgcgagccgt ccacgcatcg gccgacattt ccgcaacggt ataaagatta
    24421 tcttgctgtt gtaaatcggc aatttgctgt tcgccgccga ttaagttgac ctcataatat
    24481 ccccagtcac tgaaatgttc cgtagcaaga atatcggcat acacgccctg atgcgcacgg
    24541 tgaaatgcac caaagcctaa atgaataatt cttggggcca ggttattacg atcataaaca
    24601 gggagtgtcg cttttgctga taacaaatta tttcccataa caattcctta aatttaaata
    24661 tggcaagcta tatgttttgt tatatgaata aaaatcccct ctccggtaag agaagggatt
    24721 gagggtttac agacttctgg aaggttgcgc agctcttaca acacgcggtt gatcttccgc
    24781 agcgtcttcc agcgcactta aatcacggtc tttcacctct ggcattttca gcgcagagat
    24841 taaaccaatt actgaatatg ccatgatcat aatggcgatc ggataccagg attccgtcat
    24901 ggtgcagaaa atacccgcca ggataggacc aaaaccggaa gcgataagac caccaatttc
    24961 tttagaaata gccatccggg taaagcggtt tttacagccg aacatttctg ccatggtaat
    25021 gttttccaga gcaaataatc ccagcaccgc acagttatga atcacaatca gtgcaaccat
    25081 aatggtgctc ggggcatagc ttttatctac aatgatagaa agcattggcc atgccagcac
    25141 aatcgcggag gtattcataa taatatacgg gatccggcga ccaattttat cggataacca
    25201 accaaggaac ggaatggtca taaagccgag aatcgaacta atcatcaatg catctgttgg
    25261 aattgctttg ttaaacaata acgtctgcac taaatagcct gcaaggaaag tctgaattaa
    25321 cccggagtta cccgcctgac caaaacgcag ccctgttgcc agccagaagg atttgctctg
    25381 gaacatgcta ccagcaggtg caggttttgc tgtgggttgg ttgctgtcat taaccttctc
    25441 aaagaccggg ctttctttca gattcatacg taaccagata gcaaagacca tcacgacaac
    25501 gctcgccagg aacggtatac gccatcccca cgccagcagt tcctctttac tgagaatgaa
    25561 gaacataaag gcccagattg ccgttgcgct caaggttccg cagttagttc ccatagctac
    25621 aaatgaggag ataattccgc gcttaccttt tggtgcatat tccgccagca tcgtaccggc
    25681 accggaaatt tccgcacctg cacccaaccc ctgaataata cgcagcgtca ccagcaagat
    25741 gggggcaaaa acaccaatct gtgcataggt cggtaacaca ccaattaagg tggtacagat
    25801 ccccatcatg gtgatggtaa taaagagcac ttttttacgc cctattctgt cgcccatttt
    25861 gccgaaaata aatgctccga caatacgcgc cacataacct gcaccgtagg ttcccattgc
    25921 caaaattaac gccattgccg ttgatgattc aggaaaaaat atttcatgaa acactaacgc
    25981 tgcgccgagc gaatataact ggaaatccat aattcacaga tgttttttct cattctgtgg
    26041 tttccttggc gctttctagg ttttttcaga tagttacatt ttttcaaaag catcctgagt
    26101 tcgatcttac tatctacctg tggcctattt ctgtcccata tatgccccaa aaaacacccc
    26161 aacagataag cagtcttttc atggtgttac gcgtaaaaac aataacggct tgaaattatt
    26221 caatacactc tcgaaagtgc atcagccaac cgcagaacgt tattgcatac aacgtttctg
    26281 cggcatatca cgaaacgatt acttaatgac tgggacttca gaccactcaa tatccggtac
    26341 aattatgtat caacgcagtt taacaacacc ctatatttaa tccagagtgc gagcaacgtt
    26401 gtccctttct cttttacact cttaaaatat acgattactt aagtgaagat gcttgacgaa
    26461 tcgccaaata aaacatcttc atcagcataa aaatccatat atgattgaaa atttagtcat
    26521 taataccatt catatctgaa tactaaccta atacgcttac taaccgtgag aaatcttttt
    26581 tttcgactta atcactcgaa ataaactttc actcaattga aaacaattag ctacaattat
    26641 cattgcaata tcaaatcaaa cataacaact tgcataaatt ctcatgggat acagcatcag
    26701 ttgtgccaaa cacataagga attgaaatgg acggagaaag ctttaacttt gatgacaatg
    26761 atattgaatt tattgcttca atgtatgcat catgtaaatt atcaggaagt atcgccccca
    26821 taatgcatat aatggttagc attcccaaaa ataagaaaaa acaattctac gaacgagttg
    26881 agcattattg tagtttatat tccaataaaa acccaccttc ttaaattcgc tgcattctag
    26941 tggattccat taagaaaaca atgacataca ctcaaacaca tcttttttat caacagtgaa
    27001 tttcagttcg gccagttttt accagctatt ataaaaatag cacccatata aagtttttac
    27061 cccacaggta cagctggcca ttcgataacc gcactaagga agtgccaaca cgacatcagc
    27121 aagacccgat atttcttcca tgccgacagc aacgatgtta aggttaaatt aattataaaa
    27181 aaatatatta taattccatc ttcgcattaa attttaacct ttgatctcca cacggaacaa
    27241 gttgcatagg tgactatatt aagaaaactt ataaccacca aaaacatttg ctttgcaagc
    27301 aaaaacagtg accattgact ctaagaaaat tccgatctat tgttagtgtc atcaaagtta
    27361 cttaacagta ttccgaaatg aaaatacaaa gcaagctaat tgtcttatta aacattatca
    27421 ttgcatttat attatttgag cttttagctc aaaaatttgg tttgagtaca ttattttaaa
    27481 gcaagataaa caatatcggg cactctagtt tttataatta acaattaaac gcccataaaa
    27541 ttttatttac ttttcttcgc tattgtcata tcttaatata gtggcttacg ccactctact
    27601 ttcatcatat tttgtacgtt tatgtactcg ccatgaagct ctttcatggc gatttttatt
    27661 cttaatccaa gaacatactc cctacctata gcaaaatcac tttttgaaca aaagaataac
    27721 gtatttaggt aatcattacg aatttaccct ataggtgcta ctggccattc aatatccggt
    27781 gcagttgttg tattaacacg gttcagcaac acccgatact tcttccaggc ttccagcaac
    27841 gagatttctt cctccgttgc gatttccaga tctacagcat cctgaagtgg cgcaatatgc
    27901 tcactggcta cctgcatcag gctgtttttt gtttcttctg cctcccggat ccggaacagt
    27961 ttttctgctt ccgtatcctt cacccaggct gtgccgttcc acttctgata ttcccctccc
    28021 ggcgacaacc aggtaacatt ttccggtaac ggaccgagtt cagaaataaa taacgcgtcg
    28081 ccggaagcca cgtcataaac cgttttaccc cgatggtctt caacgagatg ccacgatgcc
    28141 tcatcactgt tgaaaacagc cacgaagcct gccggaatat ctggtggtgc aatat
//
LOCUS       DAEVZA010000056        28089 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEVZA010000056 DAEVZA010000000
VERSION     DAEVZA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.056"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(61..450)
                     /gene="mutT"
                     /locus_tag="LAF92_002442"
     CDS             complement(61..450)
                     /gene="mutT"
                     /locus_tag="LAF92_002442"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBI9791435.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(510..3215)
                     /gene="secA"
                     /locus_tag="LAF92_002443"
     CDS             complement(510..3215)
                     /gene="secA"
                     /locus_tag="LAF92_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBI9791436.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3277..3789)
                     /gene="secM"
                     /locus_tag="LAF92_002444"
     CDS             complement(3277..3789)
                     /gene="secM"
                     /locus_tag="LAF92_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBI9791437.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="LAF92_002445"
     CDS             complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="LAF92_002445"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBI9791438.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="LAF92_002446"
     CDS             complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="LAF92_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBI9791439.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="LAF92_002447"
     CDS             complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="LAF92_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBI9791440.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="LAF92_002448"
     CDS             complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="LAF92_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBI9791441.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="LAF92_002449"
     CDS             complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="LAF92_002449"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBI9791442.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETEYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9254..10729)
                     /gene="murC"
                     /locus_tag="LAF92_002450"
     CDS             complement(9254..10729)
                     /gene="murC"
                     /locus_tag="LAF92_002450"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBI9791443.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFNEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10783..11850)
                     /gene="murG"
                     /locus_tag="LAF92_002451"
     CDS             complement(10783..11850)
                     /gene="murG"
                     /locus_tag="LAF92_002451"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBI9791444.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="LAF92_002452"
     CDS             complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="LAF92_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBI9791445.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13091..14407)
                     /gene="murD"
                     /locus_tag="LAF92_002453"
     CDS             complement(13091..14407)
                     /gene="murD"
                     /locus_tag="LAF92_002453"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBI9791446.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="LAF92_002454"
     CDS             complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="LAF92_002454"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBI9791447.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15486..16844)
                     /gene="murF"
                     /locus_tag="LAF92_002455"
     CDS             complement(15486..16844)
                     /gene="murF"
                     /locus_tag="LAF92_002455"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBI9791448.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16841..18328)
                     /gene="murE"
                     /locus_tag="LAF92_002456"
     CDS             complement(16841..18328)
                     /gene="murE"
                     /locus_tag="LAF92_002456"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBI9791449.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="LAF92_002457"
     CDS             complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="LAF92_002457"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBI9791450.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="LAF92_002458"
     CDS             complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="LAF92_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBI9791451.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="LAF92_002459"
     CDS             complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="LAF92_002459"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBI9791452.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="LAF92_002460"
     CDS             complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="LAF92_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBI9791453.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22462..23466)
                     /gene="cra"
                     /locus_tag="LAF92_002461"
     CDS             complement(22462..23466)
                     /gene="cra"
                     /locus_tag="LAF92_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBI9791454.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23548..23634)
                     /locus_tag="LAF92_002462"
     CDS             complement(23548..23634)
                     /locus_tag="LAF92_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBI9791455.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="LAF92_002463"
     CDS             complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="LAF92_002463"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBI9791456.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="LAF92_002464"
     CDS             complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="LAF92_002464"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBI9791457.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGVSRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDAHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25983..26027
                     /locus_tag="LAF92_002465"
     CDS             25983..26027
                     /locus_tag="LAF92_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791458.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(26182..27126)
                     /gene="leuO"
                     /locus_tag="LAF92_002466"
     CDS             complement(26182..27126)
                     /gene="leuO"
                     /locus_tag="LAF92_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBI9791459.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            27786..27872
                     /gene="leuL"
                     /locus_tag="LAF92_002467"
     CDS             27786..27872
                     /gene="leuL"
                     /locus_tag="LAF92_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBI9791460.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGSQH"
     gene            27965..>28089
                     /locus_tag="LAF92_002468"
     CDS             27965..>28089
                     /locus_tag="LAF92_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791461.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDV"
BASE COUNT         6761 a         7951 c         6712 g         6665 t
ORIGIN      
        1 acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac
       61 ctacagacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat catcggcatt
      121 aagaccgact agcgacatcc actcaccggg ttgcccttct ttaccccacg gctccccttc
      181 ccagctttcg accagccaga accacagagt tatatgcctg tccgggaatt catattccag
      241 tttttcaaat agcgaaaaat gttgtggggt aatcccgact tcttcctgaa gttcacgcac
      301 caccgcctgt tccggcgttt cgcccatttc aattttaccg ccgggaaact ccagtttatt
      361 cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt tcattgttct cgttgcgaat
      421 aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat
      481 cctgcgcctt ttacttcaac tgttagcttt tattgcaggc gaccatggca ctgcttgtat
      541 tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgctc accggtttgc
      601 gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg ctgcatttgc
      661 gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc ttcaggcata
      721 cgtacctgga ctttgctcag cgtgctgata acttcatatt ttaacgactc cagcatcgct
      781 gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg tgcatagcca
      841 cgcaggtgga taccctgacg cagatagtcc atcgccgcca ggtgctcttt ccacagagag
      901 tcgagagttt gcagcatgac gcctttttcg aagtgacgca tcatctcagc accaaccact
      961 tcttctttac gctgatacac ttcgatggac tgcgccagaa ttcgctcacg cagcgtctct
     1021 tcatgcagtt ctggttcttt atccagccac tcggcaattg gcaaatcgag gtcgaaatcg
     1081 ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagtga ctgtggcgga
     1141 atgtaggcat caatggtcgc tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc
     1201 acatcgctga catccagcag ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta
     1261 gccacgtcat catattccag cagttgctta cgaatgtcga agttacgact ttcaacttta
     1321 cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc gcctggcttc
     1381 atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg catcagcgca
     1441 tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc agaacgaccg
     1501 cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat atgcaggcca
     1561 cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc
     1621 tctgcggtcg gattttccag cgcggcaact tctgcctgcc agctaccacc gagcacaata
     1681 tctgtaccac gacccgccat attggtcgcg atagtcaccg cagccggata acctgcctga
     1741 gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacgttgtg cttaataccg
     1801 gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt acccaccagc
     1861 accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg aattttttcc
     1921 gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg gttggtcgga
     1981 acaacgacgg tatccagctt gtagatggag ctaaattcga aagcttcggt atcagcagta
     2041 ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt gatcgaagcc
     2101 agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc ctggtgcaga
     2161 ccatcggacc agcgacggcc ctgcatggta cgaccggtgt gttcgtcaac gatgataact
     2221 tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca gcgcatgagc gcgcagcgcc
     2281 gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc cccttcatcc
     2341 atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc ggtcaggttc
     2401 acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt ttcggagtct
     2461 tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata catttccgag
     2521 ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat caggatggag
     2581 tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc agggctgaac
     2641 gccatattgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca
     2701 gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat accgacagtc
     2761 aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc caggtagtcg
     2821 ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt
     2881 gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc gttaagaacc
     2941 ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt acttgcctca
     3001 cgtaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt ttccagacgt
     3061 gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg agagtttttc catctccggt
     3121 tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg atcgttacga
     3181 ctaccgaaaa ctttagttaa caatttgatt agcataataa aatctcaaac gccccgcgtt
     3241 gcggagttaa taaaatgaag taaaggttta ttgttgttag gtgaggcgtt gagggccagc
     3301 acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt gtggggtaaa
     3361 atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg tcagcagcgc
     3421 gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt cttcagcaac
     3481 gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg attaccgtgc gaatggcatg
     3541 ttgatgccag taatcaacgg aatagttcga attcgggcgg cgtgtgttcg cttccagcaa
     3601 ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc
     3661 gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg ccgcaaccat
     3721 ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat
     3781 tccactcacg ttattgccat cccgatcgta ttcaaacgta tttagcgcac aaatcttatc
     3841 attaaagcct gcagtccaca gcacgaatga agataagttg tgcggaaaag tgcttaagaa
     3901 aacagcgttc gcaccgtttt ttatagctct tataaaattc aaaaaaaaac gactggttca
     3961 cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt
     4021 tatgccagta cagctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag
     4081 gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt
     4141 gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag
     4201 tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct
     4261 tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac
     4321 aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg
     4381 atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca
     4441 atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggcttaaat
     4501 tcagcccact tatcgccatc ttcgacacga acagtctctt tgatgcgaac aaattttttg
     4561 gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg
     4621 ccgtccatga tcggaatttc cggcgcgtta acttcgataa caatgttatc gatgcccaag
     4681 cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc
     4741 agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt
     4801 ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc
     4861 agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt
     4921 gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga agtgtactat
     4981 acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta
     5041 atcagcttgc ttacgcagga atgctgggat atccagataa tccggctctt tcgcagtttg
     5101 cggcgcattg tcattcacga ctttagcaac cggcttctgc tcctgggtca gcggagccat
     5161 cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt
     5221 gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc
     5281 attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc
     5341 acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgccgtgat
     5401 gttaaccagc acgccgcgcg cgccagacag gtcgatatct tccagcagcg gagaagagat
     5461 agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag aacccatcat
     5521 tgcgtagccc atctcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc
     5581 cggacgagta atcagttcag cgataccttg cacagcgcct ttcagtacat cgttcgctgc
     5641 gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt tgtcgttcgg
     5701 gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct ccgcgaatgc
     5761 catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc
     5821 caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac caccacccat
     5881 acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc
     5941 ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat
     6001 accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt
     6061 atttaccgcg aagaattcaa caccttcaat gcgctcgcgc accatgtgtt caacagcatt
     6121 accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg
     6181 ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc
     6241 tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac
     6301 tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata
     6361 gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt
     6421 aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc
     6481 aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt
     6541 aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag
     6601 cagctcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg
     6661 cggacgacca cctacgctcg gcacttccac gctctcatct tttccaacga tggaacccag
     6721 cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta
     6781 agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt ggcgcaatgc
     6841 cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc
     6901 cagttcacgt tcatcttccg tcaataccga ataacttgat gccagtccgg caaatatcag
     6961 ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg ccatatcgtt
     7021 gtgacatgtg atcaggtgca cttttgcctg catccgcacg cccgaaagtc ctaccggatt
     7081 cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg
     7141 ctcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt
     7201 cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc
     7261 agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg cctggtcaat
     7321 ggcgcgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc
     7381 acgtgacggg cagctgccca cgccaatgat attgaccata ccgtcgggca gaacttcccc
     7441 tactaaagcg gcaaccttcg cggtaccaat ctccagtcct actaccagtt ttctgtccgt
     7501 cgccttgatc attgttgttc tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc
     7561 ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc
     7621 cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgagcc
     7681 aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac
     7741 tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc
     7801 atctgcccca tttcgcgata gccctgcaac acttcattgg cgctgccttc cgggccatac
     7861 agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc
     7921 gcgtctacca tgtgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc
     7981 ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt
     8041 tctatttgcg tctggatgat gttgacatcc tgggtcataa aggtacccgg ctcacccaat
     8101 gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc
     8161 agctttgaga gcggcaggcg ttgcgcatct tccatccagc ccaacacgac ccagccgctc
     8221 accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta
     8281 ttgcggcgag aagaaacctc ttcttcgcta tttcgcgtgt tcagagcagc ctgcgacata
     8341 ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg
     8401 tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg cttccagcag
     8461 ataaaactgt ccatcgctgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa
     8521 cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg cttccagacc
     8581 tgcggggcag aaatactctg tctcatcaga gagatacttc gcctcataat catagaaggt
     8641 tccggacggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa
     8701 ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct
     8761 taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc
     8821 gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta actgcttatc
     8881 gctcaggcct ttttcaaact ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc
     8941 ggcaccttgc cacagcagtt tgctgcgtag tttatccatt gaaagcgcag atgccatcac
     9001 tccgctgccg gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc
     9061 accgccgcga ccgtgtagcg cgataaacac tttctggaag cccatcgact tcagttgcgt
     9121 cacgtcgact tctttcgggt cgacaggata cgcgtcaata ccgccttcac gcagcccggc
     9181 taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaggtcc cgcccaacag
     9241 gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt
     9301 cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt
     9361 taccggttaa taccggtgcc agcatctcgg ctacctgcgc cgggtccggc accagaatgg
     9421 gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa
     9481 ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca
     9541 cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca
     9601 ttaccaggtt tttatccggc cagcctgcac gcgctgcttt aatagtggcg tccacttccg
     9661 tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct
     9721 ccagcgggaa ttcaccgagg aaatcaaaac ggcgcccagt cccctggaag ctctccagcg
     9781 cacgtaaaat agcctcgtca tcaattccct cttccgtagc aaccgcaacc gcagctgcgg
     9841 cgttcagcgc gttatgacga cctggcgcat tcagggtgac gcgcatcggc tctttgtcct
     9901 ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt
     9961 cggcatcttc gttgaagccg taagtcgtgg tctgacgtcc cactcgcggt aacaattcgc
    10021 ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaacggcagg ttgtgcagaa
    10081 aattaataaa agtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg
    10141 cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct
    10201 catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt
    10261 ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa
    10321 ccatcgcggt ggttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac
    10381 gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg
    10441 cgacaatttc cgggttatcg gcagaaatcg cgctggaaac aacgaccacg ctggcatcac
    10501 gtacgttttc cgggcgatgg ttgaaataaa tcgtcgcccc aagattcatt aactgttgcg
    10561 tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt
    10621 cggcaatacc gcccataccg gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac
    10681 gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca
    10741 ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc gggcagcccg
    10801 gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc
    10861 tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga cagcatccac
    10921 gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt
    10981 ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc
    11041 ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg acgacatccg cccacgcata
    11101 cgccgccgcc atatcatcaa taaattccgt cactttatgc tgcggttgcc ccgcttcggc
    11161 atacgcctgt tcaacggatt gttgcgaacc tttaccgctc tgatgccaga tagtgactga
    11221 atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg cgccctggga
    11281 accacccact accagcacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa
    11341 cggcagcgcc agcacatcgg tacgcaccgg gttacccacc acttccgcat tagggaaagc
    11401 gcctggaaac gcctgcatca ctttggtggc aatcttcgcc agccatttat tggttaagcc
    11461 cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc
    11521 acctggacct gatacatagc cgcccatacc gagtaccacg tcaggtttgt acgctttcat
    11581 aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt
    11641 tatacctttt ccacgcagac cagagatacg aatgaaatca atttcgatgc catgttttgg
    11701 cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc
    11761 cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc cgcctgccat
    11821 caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc
    11881 tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt
    11941 aagctcgaac caccgtaact gatcagcggc aatgtcagac ctttggtcgg taacatcccc
    12001 gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat agaacaggcg
    12061 agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc ggccaatcga catcgcgcga
    12121 aaagcgacga agaataccat taaaagtgcc agcaccacac cgacataccc cagttcttcg
    12181 ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc
    12241 gagttaccta aaccttgtcc ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt
    12301 aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt aacacggcgg
    12361 atacggtacg gttcggcgag tatcagcaac acaaccgctg aaatgcccat accgataatg
    12421 gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac
    12481 aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa caccagaatc
    12541 acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc
    12601 agatagttgg cgatataaca aaacagcgac agttttgtca gctccgcagg ctggatacgc
    12661 agcaaaccga gatcgatcca acgcgatgcc cctttaaccg agctacccac taccagcacg
    12721 atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg ccagaactcc
    12781 atcggcagac gcagcgtaat gatcgccaga atgaatgcca ggatcagata gacgccatca
    12841 cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc ctatgggcat tgacgccgag
    12901 gtcaccatga taaagccaat cgccgcaagg ccgaaggtca gccacagtaa ggtgcgatcg
    12961 tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc ttttagcgcc
    13021 gtggagatcc agaccaggat actgaatcct ggcaggcgcg gcattttcag gcgagggaga
    13081 gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa
    13141 agttcttgaa ctgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct
    13201 gaacacgcgg agccagcaag cgcatcgcct gttccatagt ttcggtttgt tctgccactt
    13261 ccgggcgtag cgccgccagc tgcgcaccgt cacgaccaaa acagtacaaa cgcacgttgt
    13321 cgccattcag atagcgcgcc agtggactga agtccgccga tttaccatcg ccgcccagca
    13381 acaaatgcag cgtgccgtct acctgcaagc cattcaacgc cgcttccgta ctgccgacgt
    13441 tagtggcttt cgaatcgtta atccagcgta cgccattgtg ctccagcacc acttcaaagc
    13501 gatgcggcag accggtgaag gtggttaacg ctttcaggct gctggcgcgc ggcaaaccag
    13561 ctgcgtctgc cagtgccagt gctgccagcg cattggtgta gttatgctgc ccggaaagtt
    13621 tcatctcttt cacattcagc actttctcgc ctttaacccg cagccaggtt tcgccctgct
    13681 gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt tcatccgcac
    13741 cgcgaatcgg cattgttaag gcatcatcag cattaaccac gcaaactttc gcgttttcgt
    13801 aaatgcgcag ttttgctgca cgatactgtt gtaaaccaaa cggatagcga tccatatgat
    13861 cttcagtcac gttcagaatg gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct
    13921 ggaagctcga cagttccagc acgtacagtt cgcactcagc atcaagcagc atcaacgctg
    13981 gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttagcc atttcaccca
    14041 ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg
    14101 cttcgcgaca gaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc
    14161 ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca
    14221 gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc
    14281 caggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt
    14341 ccacgcagga aagcccggtg aggcccaggc cgataatgac gacattttta ccctgataat
    14401 cagccatgat taacgtacct tcagcgttgc cagaccaatc agaaccagca tcagcgaaat
    14461 aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg
    14521 aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat
    14581 gacagaaagc gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg
    14641 tagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccta catcgcccat
    14701 aaagacctgc gccggatagg tgttaaacca caggaagccc agtcctgccc cgactatcgc
    14761 cgtgcagaca atgaccagtt ccccggcgtg tcgcagatac ggtatgtgta aatagctggc
    14821 aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt
    14881 cggcataatt gccaggccgt cgagaccatc ggtcaggttt accgcgttgc cagtacccac
    14941 aatgacgaag taagccagca gaatgtagaa cagccccagc tgcggcatca catctttaaa
    15001 gaatgggacc accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca gggcgaaggc
    15061 gacacccagc gcaatgaccg acatccagaa atacttccaa cgagcgatca accctttggt
    15121 gtctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag
    15181 caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca gtacggagat
    15241 cacaatcgcc gtcaggatca taatcccgcc catggtcggc gtaccgcgct tgctgaagtg
    15301 tgattcagga ccgtcgttac gcaccacctg accaaaggaa agtttttgca aatgagcaat
    15361 catacgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg
    15421 aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag
    15481 ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcggcacta
    15541 cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc
    15601 gtaattaacg cagttttatc agcaaaatgt tcgccaacgc cgctggcggt gctgatagca
    15661 tggctttgtt tacccacgct taacacgcgg tcaataccag cagcttttgc cgcctcgccc
    15721 acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc
    15781 acgcggtagc ccggcatttc agccagtacc tgaacagcgg cggtcattga accgacattg
    15841 gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttggatggg gaacagacgg
    15901 cctggaacag ctttcagatt tgccagcccc gctttgatag catcaagcgt tgcgcccacg
    15961 gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc
    16021 agcagaacat cgacgctacc ggttggggtt tgtagggtaa attccgtacc gtgcgaggtc
    16081 acatggatat tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa acgccacact
    16141 ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc gttcataatg
    16201 gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca
    16261 agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc agcttccggg
    16321 cgagtcagac tcacagtcca ggctatttcg ccctgatggt tcgcgccaag ttcaataact
    16381 gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg
    16441 agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc cgtcatctct
    16501 ttaacggagg ttttgccgga ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg
    16561 cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc
    16621 aggtcgatgt ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg
    16681 tcggcaaaat cgtgggcatc aaaacgttcg cctttcaggg caacaaacag gcagcccggc
    16741 gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc ttgcagttca
    16801 ccgttgagaa tgtcggtaag ttggctaagg gttacgctaa tcatgcaatc acccccagca
    16861 gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtaat
    16921 cttcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc
    16981 aagtcaccgc ttcagcacgg ccttccatca ctttggcatg tccggcatct aacattcccg
    17041 ccagaatatc gttgatgatg gcacgcggtt cttcggtacg cgggttatcg tccgtcacca
    17101 ccgccacgtc agcaaactct tcggcaattg cgcccatcag tggacgctta cctttatcgc
    17161 gatcgccacc acagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct
    17221 gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc
    17281 ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttca
    17341 gcagatcagc cagtggatag ccgagtgcca gcagtgtcgc cagcgcgagc agcaggttgc
    17401 tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccaa cttgagctaa
    17461 agcgaatcgt cgcaccgctg tcgtgatagt tcacgtcgat cgcttttaac cagcgtccgt
    17521 gacagttcgg attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca
    17581 gccagcggcg gcccacttca tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag
    17641 aataaagcag ccatttcgca gcttcgtagt gttccatatc accatgataa tcaaggtgat
    17701 cgcggcttaa gttggtaaag accgacgccg caaatttcaa tgccgccaca cggtgctgta
    17761 ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc gccctgctcc accagccccg
    17821 ccagctcatg ctgaacatcg actgcggaac cggtagtatt ttctgtcggg atcactttcc
    17881 caagcagacc gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttggctcc
    17941 actgcgccag cagctgggta gtcgtggttt tgccgttggt gcccgttacg cctacgagac
    18001 gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga
    18061 gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct
    18121 catctttcgc ctctgcaata atggcagcca caccttgcgc tatcgcctgc gggatatatc
    18181 gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac
    18241 ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa
    18301 gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca
    18361 tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc
    18421 atgatggcac caaagaccgg cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga
    18481 tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta
    18541 taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt
    18601 ttaatggcga tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc
    18661 acgctttcca tcatatgcac cacagtgcgg acaatggatt ccgggaagac acgttcaccg
    18721 ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata
    18781 gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag
    18841 gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg
    18901 accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacgct
    18961 gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc
    19021 ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga
    19081 taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatt
    19141 accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc
    19201 atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc
    19261 gccagcactt caccggtgtt gacatccacc agcacggcgc taccagattc agccttgtta
    19321 aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc
    19381 agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca
    19441 tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc
    19501 ttctcaacgc cctcaatccc ttgactatcg acgttagtaa agccgatgag gtgagcagtc
    19561 acttcgccgg acggatagta acggcgagac tcttcacgca gatgaatccc cggcagtttc
    19621 agttttttga tgtagtccgc catgtcaggg ttcacctgac gcgccagata aataaagcgc
    19681 cctttcgggt tggcgttaat gcgggctgaa agctgatcca gcggaatatt gagcgcgtta
    19741 gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg
    19801 tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta
    19861 atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc
    19921 accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc
    19981 gccaggagaa tacagccgca taacaacgca aaacgccaac taataaagtt ggcatgttct
    20041 tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat
    20101 ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg
    20161 gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc
    20221 cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa
    20281 cgggtatggt gcgccgtggt taccacggtc accgccgtca aaataatgca aatgaacagg
    20341 cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg
    20401 cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc
    20461 atgcgttcgt cctctctgcg atacgcagaa ctgaactacg ggcacgagga ttctcagcca
    20521 cctcttcttc gcccggcatt aacttgccta atgctcgcag ctgacggcca cccagttttt
    20581 tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac
    20641 gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc
    20701 cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac
    20761 tgtttaccca aatgcgcacc gcctggaagg tacgagtcgc gggatgttta aacttatctt
    20821 tcaccggcgt tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt
    20881 cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg
    20941 ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact
    21001 gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc
    21061 gctcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct
    21121 tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga
    21181 tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga
    21241 tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc
    21301 gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt
    21361 catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc
    21421 agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt
    21481 tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact
    21541 tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca
    21601 tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg
    21661 ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg cagggggtaa
    21721 agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca tttgaccggc agcgttctca
    21781 agcagctgtt cccggtaacg ggtaggcact gataagcgcc ctttgctgtc gagattgact
    21841 aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc
    21901 ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc
    21961 gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg
    22021 cgcagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcaccgttt
    22081 taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca
    22141 ttttaggaca tatcttcccc ggttaacagt ccctgttgcg tctgtgtggc gcttgatgcc
    22201 agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat cggcaattca tctctgcatg
    22261 taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg
    22321 tgagttaatt aacgggcggg tttaaaagaa cttatcaata atagtcagga atagtcttat
    22381 ttactttaag gatattgatg tccattcccg tactctacgc gccagaggga aattcacctg
    22441 gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca ccgcggcgat agagattacg
    22501 tttaatgcgc gttaaaccag gttttggctt acgcggttcg tccaggcttg ccaggacaat
    22561 ctccagcaca cgctctgcga catcgcggtg acgttgagcc actgccagca ccgggcactg
    22621 taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt
    22681 accatcgcga cgtagcgtga catccatcac cccttgcaac aacgcaaacg acgttgtgaa
    22741 cagcgcctgc ggcatcggat gcgtttccag ccatttttcg aataactggg cagccgcctc
    22801 ccgctcatag ctgttggcat acaggaaatg cacttcgcgc ggatcatctt tccaggcagt
    22861 acggaaacct tgttcacgca ggaagctgac agaaagctcc ggcagcgcgc caagataaag
    22921 caccgtctcg gcgggaaact tacgtaactc ttccgccagc atttcggcat catcctgatc
    22981 ggcaccaacc acgctggtga aatgttcacg atcgagggcg cggtccagcg cgacaatcgg
    23041 gaacgggtcg ttagcccagc gttgataaaa aggatgctca ggtggcaacg acgtcgaaac
    23101 aataatggca tcaacctgac gctgtaaaag gtgctcaatg cagcgcattt cgttgtctgg
    23161 ctgatcttct gagcaggcaa tcagcagttg ataaccccgt tgccgcgcct ggcgctcaag
    23221 atagttagcg atgcgggtat agctggtgtt ctccagatcg gggatcacaa gaccaataga
    23281 acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc gggtggtaat tgtgctcacg
    23341 caccacagcc atgacttttt caacggtttt gtcgctcaca cggtattgct tcgctttgcc
    23401 gttaataaca tagcttgcag tggtccgcga cactcccgcc agccgagcga tttcatccag
    23461 tttcacaatt gccccttgcg taaaatgtaa aaaccatagc cattgtactg gtatgggtta
    23521 aattcattaa catctaagcg cagaaaatta actgcggcaa ctgcttttat tccaggttcc
    23581 cactgatttc gcaaaaaaag cccaacgtga catgttgggc tgcaaattgc gcactgagat
    23641 cattctcaac gcattatttt atcgccgcgc gaaagtccga ccacaccaga gcgagcaact
    23701 tccacaattt tcgccacatc gcgaatcgat gctaaaaatg catcaagctt atcgctggtg
    23761 cctgctaatt gaacggtata aagcgagggg gtgacatcga taatttgccc acggaatatt
    23821 tccgtattac gtttcacttc gtcacgcccg taaccgctgg cctgaatttt caccagcatg
    23881 atttcccgct caacatgcgc gccctgcccc aactcactca cgcgcaagac atcgaccagc
    23941 ttgtgtaatt gcttttcgat ctgctcaagc actttttcat cgcccacggt ctggatggtc
    24001 atacgcgata atgtcggatc gtcggttggc gcaacggtca ggctttcaat gttgtagcca
    24061 cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg attcattttc gagtaagact
    24121 gataatatcc ggcgcataat caggttctct ccgttttgct taaccacatt tcatccattc
    24181 cgcccccgcg aatctgcatc gggtagacgt gctcactgcc atcgacggta acatcaacaa
    24241 acaccaggcg attattgcgc acctgttcca gcgcctcgct aagtttgctt tccagctcat
    24301 gcggatgaga gatctggatc ccgacgtgac cataggcttc cgccagacgg acgaaatcgg
    24361 gtagtgattg catataagat tgtgaatgac ggccggaata gatcatgtcc tgccactgtt
    24421 tcaccatccc cagatagcgg ttattgagat tcaccaccag taccggcaac tcgtattgca
    24481 acgcggtaga cagttcctgg atgttcatct gaatactgcc gtcgccagtg acgcaaacca
    24541 cggtttcttc tggcaacgcc attttgacgc ccagtgccgc aggtaaacca aaacccatcg
    24601 tgccgaggcc accggaattg atccagcgac gcggtttgtc gaatggataa taaagcgcag
    24661 caaacatctg gtgctgcccg acatcggacg tcacgtaagc gtctcccttc gtcaaccgcc
    24721 aaagagtctc gatcaccgcc tgcggtttaa tcttttcact gtgagcgtca tatttcaggc
    24781 actgacgagc gcgccactgt tcaatctgct gccaccagtc gcggatctca tccagcggtt
    24841 gatgggcgga ttcttgcgac aggagttcaa gcatttgttc gaggacctgg cgagcatccc
    24901 ccacaatcgg gatatcagca gtcacggttt tagaaatgga agtaggatca atatcgatat
    24961 gcagaacagt ggcatttggg cagtactttg ccagattgtt cgtcgttcgg tcatcaaatc
    25021 ttaccccgac ggcgaaaatc acatccgcgt tatgcatcgt catattggct tcgtaggtac
    25081 cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc cggaaacgcc cccagcccca
    25141 tcaatgagca aacaacgggc agattcaacg cctccaccgt ttctttcaac tgctgatggc
    25201 agcccgcagt gattgcccca ccgcctacgt agacaactgg tttttttgcc gccaccagcg
    25261 tttgcagagc gcgcttaatt tgccctttat gcccggaggt cgtgggattg taagatcgca
    25321 tactgaccga ctccggccag acgtagggta atttgttcgc cggattaaga atatctttgg
    25381 gtaaatcaac gaccacgggc ccagggcgac cacttgccgc cagccagaaa gcctttttca
    25441 gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc accggtcgcg
    25501 aaacccccac catgtcgcac tcctgaaagg catcgtaacc tatcaacgag gtcgctacct
    25561 gtccggaaag gacaactaat ggaatggaat ccatataagc ggtggcgatg ccggtaatcg
    25621 cattggtcgc ccctggcccc gacgttacca gcacgacgcc gacttccccg gtcgcgcgcg
    25681 ccaggccatc ggccatatgc accgccgcct gctcatgacg aactaataca tgatcaatac
    25741 cacccacggt atgcaatgca tcgtaaatat caaggactgc gcctccggga taaccgaata
    25801 cttgtttaac gccctgatcg ataagcgatc ggacgaccat ctcggctcca gacaacatct
    25861 ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa acacattaac cgtccgatct
    25921 tgcttaagca attggcagcc agagacataa acgcagggta ataagaataa acaggaggaa
    25981 aggtgaaaag aaacgataaa atcctccatt acgaaggatt aaattgacaa taaatcggct
    26041 gaatcccaca acttacagca atttctctat ttttttaatt aatcctaaaa tagagagcac
    26101 acacattcag aataaaattc tacattcaat aaaaaatgca gaataaacca gacattcatg
    26161 tctggcctat tctgcattca gttagcgttt gcaaattgag actaattgtt cttccatcca
    26221 ctgatggcct ttatcgcgcc cggcagcttc atgccaggag agataacagg ttctgctgtt
    26281 ttgttttaac ggcagcggta atacctgtaa ttctaaggat tcagcgaact cttcagccag
    26341 ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc acgctaagta cgctcatcat
    26401 tgccatgccc tgatacgcga tactggcttg cttatctacc gtgtcatacc aaggttgact
    26461 aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt tcgttataaa catcatgttt
    26521 cagtaacggg cccttaattg ttggatgatt tttgctggct accaacacca tttcatcttt
    26581 aaataatggc acgctggtaa attcaggacg atggaagtct tcataactaa tcacaaactc
    26641 cgtttcctga taacgcagct gatgttcagt gttctgattt aatgaagact tgaacataac
    26701 atgtatattt ggcgcaatct gctcaatgtg attataaatc tgcgaggtca gaatgctgtc
    26761 taacgggctg caaacacaaa gatgaaatac acgttcactg ctcgcgggtt caaaacctga
    26821 accaggcaat tcattttgta ctagttgcaa tgcctgacga actgagccaa aaagttgaaa
    26881 tgcgcgagca gtcggttgaa taccacggcc ataacgaaca aaaagctcgt cattaaacat
    26941 caccttcagg cgtgcaacag cgttactgac cgcaggttgc gacataccca gaacatgagc
    27001 ggcacgggta atgttttgct cctgcatcac ggcatcgaaa acggttaata agttgagatc
    27061 gaccatgcgt agctgtggct tgcttaactc cgccgtctct gaatgatctg tttgtacctc
    27121 tggcatactt aactccactg tcacacttaa ctccctttcc cttattggaa tgcagagaaa
    27181 taatcctgaa gaatatgatt tatcatgcat ataaaataag aaaaagcaaa atgagtaaaa
    27241 ttcggaaaac ataaagatac gggttctgat aaaaacagaa tccattgccg tggaatgagt
    27301 catttaccac agcataataa tccataatgt ttttgttgcg aaaacaatct aatcataact
    27361 accgcgaata ctcaatcatc tacaaaatgg attaaatgtg cttatttaat aattaattta
    27421 tatatttaat gcattaattc ttaacattaa ttgatcaata atattcacca aatcaatatc
    27481 aaaaaaaatc gcaaaacata taattcaata caaatcatca ggataggttt tgcaacgcgt
    27541 gcattttgtc ccctttttcc tcgttgatta gatgcaaaaa tttatgctga aatatgtcaa
    27601 ccgatgaaaa gcgtcggtag ttaagcagaa attaatatcg cttactttaa ccaccgcagc
    27661 acaattagct aattttacgg atgcagaact cacgctggcg ggacgttttt attgcgtcag
    27721 ggttgacatc cgtttttgta tccagtaact ctaaaagcat atcgcattca tctggagctg
    27781 atttaatgac tcacatcgtt cgctttatcg gtctactact actaaacgca tcttctttgc
    27841 gcggtagacg agtgagcggc agccagcatt aagccagcac gcagtcaaac aaaaaacccg
    27901 cgccattgcg cgggtttttt tatgcccgaa gcgaggcgct ctaaaagaga caaggaccca
    27961 aaccatgagc cagcaagtca ttattttcga taccacattg cgcgacggtg aacaggcgtt
    28021 acaggcaagc ttgagtgtga aagaaaaact gcaaattgcg ctggcccttg agcgtatggg
    28081 tgttgacgt
//
LOCUS       DAEVZA010000057        27573 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEVZA010000057 DAEVZA010000000
VERSION     DAEVZA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.057"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1330
                     /locus_tag="LAF92_002469"
     CDS             <1..1330
                     /locus_tag="LAF92_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBI9791462.1"
                     /translation="VFSLLGGKGLNERVGSTLEIESGSNDPMAVFLTITLIAMIQQHE
                     SSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIALPAGLYPLLALSGGILIFALT
                     TALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAWLAQIAMFLVLGLLVNPSDLL
                     PIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERVFISWVGLRGAVPIILAVFPM
                     MAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKVVVPPVGRPVSRVGLDIHPEN
                     PWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDNQLLHPTGSTRLREGDVLCVI
                     GRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYADVALIYGLEDGREYRDKQQT
                     LGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLKIGVRVAEEEAES"
     gene            complement(1716..2786)
                     /gene="dadX"
                     /locus_tag="LAF92_002470"
     CDS             complement(1716..2786)
                     /gene="dadX"
                     /locus_tag="LAF92_002470"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBI9791463.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(2796..4094)
                     /gene="dadA"
                     /locus_tag="LAF92_002471"
     CDS             complement(2796..4094)
                     /gene="dadA"
                     /locus_tag="LAF92_002471"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBI9791464.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            4424..5956
                     /locus_tag="LAF92_002472"
     CDS             4424..5956
                     /locus_tag="LAF92_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBI9791465.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(6008..6727)
                     /gene="fadR"
                     /locus_tag="LAF92_002473"
     CDS             complement(6008..6727)
                     /gene="fadR"
                     /locus_tag="LAF92_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBI9791466.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            6949..8490
                     /gene="nhaB"
                     /locus_tag="LAF92_002474"
     CDS             6949..8490
                     /gene="nhaB"
                     /locus_tag="LAF92_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBI9791467.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            8636..9166
                     /gene="dsbB"
                     /locus_tag="LAF92_002475"
     CDS             8636..9166
                     /gene="dsbB"
                     /locus_tag="LAF92_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBI9791468.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9212..10480)
                     /gene="umuC"
                     /locus_tag="LAF92_002476"
     CDS             complement(9212..10480)
                     /gene="umuC"
                     /locus_tag="LAF92_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBI9791469.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(10480..10899)
                     /locus_tag="LAF92_002477"
     CDS             complement(10480..10899)
                     /locus_tag="LAF92_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBI9791470.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            11272..12182
                     /gene="hlyE"
                     /locus_tag="LAF92_002478"
                     /pseudo
     CDS             11272..12182
                     /gene="hlyE"
                     /locus_tag="LAF92_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(12389..12835)
                     /locus_tag="LAF92_002479"
     CDS             complement(12389..12835)
                     /locus_tag="LAF92_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBI9791471.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLVEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(12927..13586)
                     /locus_tag="LAF92_002480"
     CDS             complement(12927..13586)
                     /locus_tag="LAF92_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBI9791472.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(13658..13951)
                     /locus_tag="LAF92_002481"
     CDS             complement(13658..13951)
                     /locus_tag="LAF92_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBI9791473.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(13963..14121)
                     /locus_tag="LAF92_002482"
     CDS             complement(13963..14121)
                     /locus_tag="LAF92_002482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791474.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            14192..14593
                     /locus_tag="LAF92_002483"
     CDS             14192..14593
                     /locus_tag="LAF92_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBI9791475.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(14713..15081)
                     /locus_tag="LAF92_002484"
     CDS             complement(14713..15081)
                     /locus_tag="LAF92_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791476.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            15601..16296
                     /gene="minC"
                     /locus_tag="LAF92_002485"
     CDS             15601..16296
                     /gene="minC"
                     /locus_tag="LAF92_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBI9791477.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            16320..17132
                     /gene="minD"
                     /locus_tag="LAF92_002486"
     CDS             16320..17132
                     /gene="minD"
                     /locus_tag="LAF92_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBI9791478.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            17136..17402
                     /gene="minE"
                     /locus_tag="LAF92_002487"
     CDS             17136..17402
                     /gene="minE"
                     /locus_tag="LAF92_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBI9791479.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(17774..18118)
                     /locus_tag="LAF92_002488"
                     /pseudo
     CDS             complement(17774..18118)
                     /locus_tag="LAF92_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(18153..18272)
                     /locus_tag="LAF92_002489"
     CDS             complement(18153..18272)
                     /locus_tag="LAF92_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBI9791480.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(18233..18418)
                     /locus_tag="LAF92_002490"
     CDS             complement(18233..18418)
                     /locus_tag="LAF92_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791481.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            18519..18692
                     /locus_tag="LAF92_002491"
     CDS             18519..18692
                     /locus_tag="LAF92_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791482.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            18694..19038
                     /locus_tag="LAF92_002492"
     CDS             18694..19038
                     /locus_tag="LAF92_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBI9791483.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            19042..19377
                     /locus_tag="LAF92_002493"
     CDS             19042..19377
                     /locus_tag="LAF92_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBI9791484.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(19434..21755)
                     /locus_tag="LAF92_002494"
     CDS             complement(19434..21755)
                     /locus_tag="LAF92_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI9791485.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGRGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYAIWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(21872..22081)
                     /locus_tag="LAF92_002495"
     CDS             complement(21872..22081)
                     /locus_tag="LAF92_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791486.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(22481..22699)
                     /locus_tag="LAF92_002496"
     CDS             complement(22481..22699)
                     /locus_tag="LAF92_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791487.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(22831..24354)
                     /locus_tag="LAF92_002497"
     CDS             complement(22831..24354)
                     /locus_tag="LAF92_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI9791488.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(24686..24934)
                     /locus_tag="LAF92_002498"
     CDS             complement(24686..24934)
                     /locus_tag="LAF92_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791489.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(25047..25313)
                     /gene="ariR"
                     /locus_tag="LAF92_002499"
     CDS             complement(25047..25313)
                     /gene="ariR"
                     /locus_tag="LAF92_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HBI9791490.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(25342..25614)
                     /locus_tag="LAF92_002500"
     CDS             complement(25342..25614)
                     /locus_tag="LAF92_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HBI9791491.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(25657..25893)
                     /locus_tag="LAF92_002501"
     CDS             complement(25657..25893)
                     /locus_tag="LAF92_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HBI9791492.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            26207..27418
                     /gene="bluF"
                     /locus_tag="LAF92_002502"
     CDS             26207..27418
                     /gene="bluF"
                     /locus_tag="LAF92_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HBI9791493.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFETIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT         7329 a         6652 c         6255 g         7337 t
ORIGIN      
        1 ggtcttttct ctgctgggtg gtaaggggct taacgaacgt gttggctcga cgctggaaat
       61 tgaatccggc agtaatgatc caatggcggt ctttctgacg attaccctga ttgcgatgat
      121 ccagcaacat gaaagcagtg tcagttggat gttcgtggtc gatattctgc agcaatttgg
      181 cctcgggatt gtcatcggac ttggcggtgg ttatttactg ctgcaaatga ttaatcgcat
      241 cgccctgcct gccggattat atccattgct ggcattaagc ggcggtattt taatttttgc
      301 tttaaccacg gcgctggaag gcagcggtat tctggctgtt tatctgtgcg gttttctgct
      361 gggtaatcgg ccgattcgca accgctacgg catcctgcaa aatttcgacg gcctcgcctg
      421 gctggcgcaa atcgccatgt tcctggtgct ggggctattg gttaacccaa gcgatctgct
      481 gcctattgcc attccggcgc tcattttgtc ggcatggatg atattcttcg cccgtcccct
      541 ttcggtattt gctggattgc tccccttccg cggtttcaat ctgcgtgagc gcgtgtttat
      601 cagctgggta ggattacgcg gcgcggtgcc aatcatcctg gccgtgttcc cgatgatggc
      661 ggggctggag aatgcacgtc tgttctttaa tgtcgccttc tttgtggttc tggtttcact
      721 gctcttgcag ggaacatcac tctcgtgggc ggctaaaaaa gccaaagtgg tggttccgcc
      781 agtgggacgt ccggtgtcac gcgttggcct ggatattcat ccggaaaatc cgtgggagca
      841 gtttgtttat caattgagtg ccgataaatg gtgcgtgggc gcggcactgc gcgatttgca
      901 tatgccaaaa gagacgcgta ttgcggcact gtttcgtgat aaccagttgc ttcatcctac
      961 cggcagcacc cgactgcgcg aaggcgatgt gttgtgtgta attggtcggg aacgcgatct
     1021 tccggcgctc ggtaaactgt tcagccagtc gccgccggtc gcgctggatc aacgcttctt
     1081 tggtgacttc attctcgaag ccagcgccaa atatgctgat gttgcgctga tatatggcct
     1141 ggaagacggg cgagaatatc gtgataagca gcaaacgctg ggtgaaattg tccagcagtt
     1201 gttaggcgcg gcaccggttg tcggtgacca agtagagttt gccgggatga tctggacggt
     1261 ggccgagaaa gaagataatg aagtgttgaa gattggcgta cgggtggctg aagaggaagc
     1321 tgaatcttaa atttaatgaa gcatctatcg ccggttgcga ttctttgctg aacgcagcct
     1381 gagcctgcaa ttgtttgtcg tccataattt aaccttcatt tgatgttgaa ttgaacatat
     1441 caaaatcagg caattacaca aatctatgta caggctccat caagggtaaa gcgtgattta
     1501 tctgaagtcg agttcgagtc aacaatgaag cgtaagcgtt ttatgtagaa cgcctgtgcg
     1561 gtgagttcag gttccgggga aatagtgtcc attcttttaa tggacactat catgggcaat
     1621 ggctctgtca cattaacaac tacagttgct gaccagccgg atggatgatt ttttcgcaac
     1681 cagaagacgc tgtctccgat ccggcttaca acaagttaca ctgtcacaac cgggacgcgt
     1741 aacgccagcg cgcacatcaa ctcatagccc accgttccgg cagcggcggc gacatcatca
     1801 attttgatct ccttgcccca cagctcaacc ggcgtaccaa tccccgcctg cgggcaaggc
     1861 gttaaatcga ccgctagcat atccatcgag acggtcccca ccgtcatggt gcgcacgccg
     1921 tccactaaaa caggggtacc ggtaggcgcg tggcgcggat aaccgtcggc gtaccctgcg
     1981 gcgacaatgc cgattcgctg ttcatcgcgc gcagtatagc gaccgccgta gcccacacgc
     2041 tcacccgctt ttagcgtctg gacaccaata atctcactgc ttagcgtcat caccggacgt
     2101 aatccggtat tggcgatatc acgccactga ccggacggcg aagcgccata caaaataatg
     2161 ccaggccgaa cccagtcaaa atgcgcttcc gggtgccaca gagtcgccgc cgaattggac
     2221 aacgaacgcc gacactccag cccttccgcc gcctgctcaa tacgcgccat cgcgctggaa
     2281 attccatcag gatgttccgc ttcggcaaaa tgcgacatca gggtcatctc gccaacattc
     2341 gccattgccc gcaactgctg ccagacggta agcacgcgat cgggctggaa gcccaaccga
     2401 ttcatcccac tgttcacttt aagataaata tccaacggtg cttttagccg cgcattttgc
     2461 agtgctttga gctgccagtt gctgtgtacg caggtggtca ggcggtgctg gtcataaatc
     2521 tccagatcct gagcatggaa aaatccttcc agcatcagga tcggcccctt ccagccgcgc
     2581 tcacgtaacg ttattgcctc ttccaggtta agtaatgcaa agccatcggt ggccccgagc
     2641 gcgctccaga tacgctcaat accatgcccg taagcgttcg cttttaccac cgaccagacg
     2701 cgcgcgtgcg gcgcggcctg gcggacaatg gacagattct gttttaatgc ctgcagatcg
     2761 aggctggcct gtatcggacg ggtcatctcg tttccttagc tgtgtgcgcc atgtaaatgg
     2821 cccggacgtg atggcgtaaa tccacggctg tagcgcgcta cgcttagatc ctcatatggg
     2881 atcgctggcg tgcgaccaga gagcagatcg cttaacaact gaccggaacc gcaagccatc
     2941 gtccagccga gcgtgccgtg accggtattc agccacagat ttttaaagcg tgtacgcccg
     3001 acaaccggcg taccgtctgg cgtcatcggg cgcaaacccg tccagaaagt cgcctgctcg
     3061 acatgaccgc cgcgtggata gagatcgcga accaccattt ccagcgtttc acgacgcggt
     3121 tgcaacagct cggtgttaaa accaacaatt tccgccattc cgccaacacg aatgcggtta
     3181 tcgaaacggg taatggcgat tttgtaggtt tcatcaagaa tggtggatac tggcgcacca
     3241 tcttcctgcg caattggaat agtcagcgag tagcctttca gcggatacac cggaatatca
     3301 acaatgcctt tgagcatcgc cgtcgagtaa gaaccaaacg ccatcacata cgcatcggcc
     3361 ttaatcactt catcgccaca cttcacgccg tagatttgct cgccgtcgca aagcagttgg
     3421 tcaacgggcg tattaaagcg gaatttaacc cccgcctgct ctgccatccg cgccagattc
     3481 tgggtaaaca gctggcagtc tccggtttca tcattgggca gttgcagacc acccgtcagt
     3541 ttgtgcgcta cttctgccag cgcgggctcc acttccgcca ggcggctgga ttccagcagc
     3601 tgatacggta cgccggcatc ttccagcacg gcgatatcgc gggtcgcatt ttcatactgt
     3661 tgttcggtac ggaacagttg cagcgtccca ccctgacgcc cttcatactg aatattggtt
     3721 tcggcgcgca atgctttcag gcaatcacgg ctgtattccg ccagacgcac catccgccct
     3781 ttgttttcca tgtagtggct ggtgtcgcag ttacgtaaca tttgccacat ccatttcaac
     3841 tggaactgcg taccgtcgag acgaaccgcc agcggcgcat ggcgctggaa catccattta
     3901 atcgctttta aaggcacacc tggtgccgcc cacggtgcag catatccggg ggagatttgc
     3961 ccggcattcg cggcgctggt ttccagggcg gcccccggct cccgatcaat gacggtgacc
     4021 tcatgtcctg cctgatttaa gtaccaggcg ctggcaacgc ctaccacacc acttcccagt
     4081 atgacaactc gcatagccac tccgataacg gtagatacag taaaagaata ataatctaat
     4141 tacatcttga taactcagtt gaaaatatta ttcaacatac ggctttttta tggtgagctg
     4201 gctcacatct ccctgactct gcggcaatat ggcgtcttta gtatctcctg ctgaacgccc
     4261 tttttatcca gcataaaatt ccgttcagaa gcggattagt ggcactctga taataagaaa
     4321 tcgcaaagaa aaaaattctc cggcgacgcg gtttttaaca ggtgttctat gcttgaaatg
     4381 aggtgctcca cacagagagc gccgacaaca atgaggtgcg cgtatggcga cgatcgattc
     4441 tatgaataag gacaccacac gtttgagcga tggacccgac tggacgttcg acctgctgga
     4501 tgtttatctg gcagagatag accgggtggc gaaactctac cggctggata cctacccgca
     4561 ccagattgaa gtgataacct cagaacagat gatggatgcc tactccagcg tcggcatgcc
     4621 aattaactat ccgcactggt cattcggtaa aaagtttatc gagactgaac ggctgtataa
     4681 gcacggtcag caaggactgg cctatgaaat cgtcattaac tctaacccgt gtatcgctta
     4741 cctgatggaa gagaacacca ttaccatgca ggcgctggtg atggcacacg cctgctatgg
     4801 gcataactct ttctttaaaa acaattactt attccgcagt tggaccgacg ccagttcgat
     4861 tgtcgattat ctgatttttg cccgtaaata tattaccgag tgcgaagaac gttatggcgt
     4921 tgatgaagta gaacggcttc tggactcgtg ccacgcgctg atgaactacg gcgtggaccg
     4981 gtacaaacgc ccgcaaaaaa tctcgctgca agaagagaaa gcccggcaga aaagtcgcga
     5041 agagtatcta caaagtcagg tcaatatgct ctggcgtacc ctgccgaagc gcgaggaaga
     5101 gaaaacggtt gctgaagcgc gccgctatcc gtccgaacca caagaaaacc tgctctattt
     5161 tatggagaaa aatgcgccac tgctggaatc atggcagcgt gaaatcctgc gtattgtgcg
     5221 taaggtgagc cagtattttt atccgcaaaa acagactcag gtgatgaacg aaggctgggc
     5281 gaccttctgg cactatacca tccttaacca tctgtatgat gaagggaaag taacggaacg
     5341 ttttatgctg gagtttttgc acagccacac caatgtggtc ttccagcccc cctataacag
     5401 cccgtggtac agcggcatca acccatatgc cctcgggttc gccatgttcc aggatattaa
     5461 acggatttgt cagtcgccaa cggaagaaga caaatactgg ttcccggata tcgccggttc
     5521 cgactggctg gaaacgctgc atttcgcgat gcgtgatttc aaagatgaga gttttatcag
     5581 ccagtttctg tcaccgaaag tgatgcgtga tttccgcttc ttcaccgtgc tggatgatga
     5641 tcggcataat tatctggaga tttccgctat tcataatgaa gaaggttatc gggagatccg
     5701 taaccggtta tcgtcgcaat ataacttaag taatctggag ccgaatattc agatctggaa
     5761 cgtggatttg cgcggcgacc gttcgctgac gctgcgttac attccacata atcgcgcacc
     5821 gctggatcgg gggcgcaaag aagtgctgaa gcatgtgcat cgcctgtggg gatttgatgt
     5881 gatgctcgaa cagcaaaacg aagacggcag catcgagttg ctggaacgtt gcccgccaag
     5941 aatgggaaat ctgtaaaaac aacaaaaaac ccctcgtttg aggggtttgc tctttaaacg
     6001 gaagggatta tcgcccctga atggctaaat cacccggcag atttttctgc atccggtgcc
     6061 aaatctcgcc actctcatgc ccatagcgac gcactgtttc gtacacctga tcgtgcgcgc
     6121 cttcactgca caacgccgac agtttgtggt agaagcccag cgccagactg cgcgcttccg
     6181 gattggcgaa atagtgacga ccaatgcgcg tatacagccc tttcatcccg ttaaggatca
     6241 gaccgtaaat cgggttgccg gaagcaaacg ccaggccgcg gaatatgttg taatccagct
     6301 cggcaaaggc atcggcgtga tcggccactt cattagcggt agccagcact tcctgcgctt
     6361 tatcgggatg ctgacgaaac gcggtgcgaa taaaaatagt ggaaatattg gtacgcaccg
     6421 acagcaaatt atcaataagc tgcggcacac tttcgtgatc cagtcgcgcc agtgtttcaa
     6481 ggatatttaa accagaggtt tcccagaaat tattcacctt cgtcggcttg ccatgttgaa
     6541 tggtcaacca gccatctcgt gccagacgct gtaacacttc acgtaacgta gtacgcgtta
     6601 cgccaattaa ttctgaaagt tcacgttctg cgggcaaaat agtcccggga gggaagcggt
     6661 tattccagat actttcaata atgtactctt ccgcgaaacc cgccgggctt tgcgccttaa
     6721 tgaccatagt gagatttcca taacacagca aaacaaagtt ggactcatca taccagaggg
     6781 ctaactacgc tgatagcaga caaaaagaag aaaaaaggga tcaaggcttc attttccgca
     6841 tctctttccc cttgactatc aagacttgcc tcactcctgc ctgaccgtta agctttgcgt
     6901 ttaaacataa aaacatcatt gattatttgt aaggtaaggg aaaccatcat ggagatctcc
     6961 tggggccgcg cgctatggcg caattttttg ggccagtccc ccgactggta caaactcgcc
     7021 ctcatcattt tcttaatcgt aaacccgtta attttcctca tcagcccttt cgtcgcgggc
     7081 tggttgctgg tagcggaatt tattttcact ctggcgatgg ccctgaaatg ctacccgctg
     7141 ctccccggtg gtctgttggc tatcgaagcg gtattcatcg gcatgaccag cgcggaacac
     7201 gtccgtgaag aggtggcggc aaatcttgaa gtcttgctgt tattgatgtt tatggtggcg
     7261 ggtatctatt ttatgaaaca gctgttgctg ttcatattta cccgtttgct gttaagcatt
     7321 cgctccaaaa tgctgctgtc gctctctttt tgcgtggcgg cagcgttcct ctccgcgttc
     7381 ctcgatgcct taaccgtcgt ggcggtggtg atcagcgttg ctgtcggttt ttatggtatt
     7441 tatcaccgcg tagcctcttc ccgtaccgaa gataccgacc tgcaagacga tagtcatatc
     7501 gacaagcatt acaaagtggt tctggaacag ttccgtggct ttctgcgtag cctgatgatg
     7561 catgccggtg tcggcactgc attaggcggc gtgatgacca tggtgggcga accacagaac
     7621 ctgatcatcg ctaaagcggc tggctggcat tttggcgatt tcttcctgcg catgtcgccg
     7681 gtgaccgttc cggttctgat ttgtggcctg ttaacctgcc tgctggtaga gaagctgcgt
     7741 tggtttggct acggtgaaac gctgccggag aaagtccgcg aagtgttgca acagtttgac
     7801 gatcaaagcc gccaccagcg tacccgtcag gataaaatcc gtctgattgt ccaggcgatt
     7861 attggcgtct ggctggtgac tgcgctggcg ttgcatctgg cggaagttgg cttgattggt
     7921 ttgtcagtca ttattctggc gacatcattg accggtgtca ccgatgaaca tgctatcggt
     7981 aaagccttca ccgaatctct gccattcacc gcactgttga cggtgttttt ctcggtagtc
     8041 gcggtgatta tcgaccaaca actgttttcg ccaattattc agtttgtgtt gcaggcatcg
     8101 gaacatgctc agctgtcgct gttctatatt ttcaacggtc tgctgtcatc catttcggat
     8161 aacgtcttcg tggggacgat ttatatcaac gaagcgaaag cggcaatgga aagtggcgct
     8221 atcacgttga agcaatacga gctgctggcg gtcgccatta ataccggtac caatctgccc
     8281 tccgtcgcta cgccgaacgg tcaggctgcg ttcctgttcc tgctgacctc tgcactcgcg
     8341 ccattgattc gcctctctta tggccgcatg gtgtggatgg ccctgcctta caccctcgta
     8401 ctgacactcg tcggcttgct ctgcgtcgag tttacgcttg cccctgtaac cgaatggttt
     8461 atgcaaatgg gctggatagc aacgctttga taacaactta ccgggcatat aaatgcccgg
     8521 tttgcctttt cgcccgataa ttgtccaatt gcttcctatt tttgctgcct cctggtggcg
     8581 cagcgaatga attggtttaa actgcgcact ctatgcatat tgcagggaaa tgattatgtt
     8641 gcgatttttg aaccaatgtt cacaaggccg gggcgcgtgg ctgttgatgg cgtttactgc
     8701 tctggcactg gaactgacgg cgctgtggtt ccagcatgtg atgttactga aaccttgcgt
     8761 gctctgtatt tatgaacgtt gtgcgttatt cggcgttctg ggtgctgcgc tgattggcgc
     8821 gatcgccccg aaaactccgc tgcgttatgt agcgatggtt atctggttgt atagtgcgtt
     8881 ccgcggtgtg cagttaactt acgagcacac catgcttcag ctctatcctt cgccgtttgc
     8941 cacctgtgat tttatggttc gtttcccgga atggctgccg ctggataaat gggtgccgca
     9001 agtgtttgtc gcctctggcg attgtgccga gcgtcagtgg gattttttag gtctggaaat
     9061 gccgcagtgg ctgctcggta tttttatcgc ttacctgatt gtcgcggtgc tggtggtgat
     9121 ttcccagccg tttaaagcga aaaaacgtga cctgttcggt cgctaatccc ttcagcgctc
     9181 ctgcgggagc gcttttttcc tgccgctata tttatttgac ccgcagtaaa tcagaacttc
     9241 gcgttgtata acgtggtgaa agcatggcac gcttcatctg ccattgttgc tggatcccct
     9301 gcccggcaaa atagagtgtt cctctgccct ctttagcatt cagtgtatcc attaccgtca
     9361 tcaattgctc actcccgggg cgcggtgcgt tgtcatcgaa taaattgagc tgcgcgactc
     9421 cctgactgaa gaaatccccc agcatcacgc ccgctttttg gtagcgatgg cccgcttgcc
     9481 agatggcatc cagagatcgc gtagcagcgt taatgatatc cctgctgtcc tgagtgggcg
     9541 tcagcagttt taccgacgcg ctattgccgt aataaggttc attgagcgca aatggtgacg
     9601 tcttaataaa tgtggagata aaccgacaat attgatgctc gctgcgaagt ttttccgccg
     9661 cccgggcagc gtaactacaa atggcctgcc gcatcgatgg ataatccgtg atgcgttcac
     9721 caaacgagcg ggaacagata atttcctgct tcgtcggtgc aaactcttcc agttgcaaac
     9781 agggttcgcc gcgcagttca cgcaccgttc tttcgagcac gacattaaaa tgtttacgga
     9841 taaaccggat atctgtatcc gccaaatcga gaacggtttt gatccccatc gcgtccagtt
     9901 ttttgctgat ccgccgtcca atcccccaga cgtcatccac ggggagagca gacattaatt
     9961 tacgctggcg ttccagattt gataaatcca ccaccccacc cgtctgccgc tgccattttt
    10021 ttgccgcatg attggcaagc ttagccaacg ttttggtctg ggcgatcccc acaccaacag
    10081 taagatgggt acgttgtagc accgttgcgc gaatttctct gccaaaatca gtcagatcgc
    10141 gacaattacg cacacctgtc agatcgcaga atgcctcatc aatactgtaa atctcgacgc
    10201 ggggcgatag ttcttccagc gtcgacatca cccgattgct catgtctgcg taaagctcat
    10261 aattgctgct aaagcaaacc acgccacagc gacgaaacag atctttttgt ttgaaccagg
    10321 gatcgcccat tttaacaccc agcgcctttg cctcagcgtt tcgggcgata acgcaaccgt
    10381 cattattcga tagcacaacc accggtttac cccataaatc agggcgaaac accgtctcac
    10441 agctggcata aaacgcgttt acatcacaga gagcaaacat cagcgcatcg ccttaacgac
    10501 gtggatcacc acaccaaaga catccagcgt atcttcgcta ctgatggtaa tgggcgagta
    10561 cgcactgttc atgggaataa gctgtaccgt cgggcgtagt tgcaattttt tcaccgtaaa
    10621 ctcgccgtca acagcagcga tgacaatatc accatggctg gcggtaatag cgctatcgac
    10681 aatcagtaaa tcaccgtcac taattccacc atcaatcata gaatcaccac ttgctttgac
    10741 gaagtaagtc gcgctgggat gctggatcaa cagttgattc agatcgatgc gctgttcaac
    10801 gtaatctgct gccggtgaag gaaagccaca ctgaacaaga tcgctaaata gcggaaaagt
    10861 cacaatttcg cggagatccg caggcttgat aaacaacata ataatctgcc tgaagttata
    10921 ctgtttttat atacagtagt ctattcttgc cagcagatca atactgattc aggctatcaa
    10981 tatttgtcgc tgcatagact gctgattttt cgttctctta tcctgtgctc acgtggcctt
    11041 ctggcgacga cgctcatcca gcagaaatga aaaatatcac ccggcaaaaa aatagaatag
    11101 aagcattcgc cataatgaca ttaaacattg tttgatattt atcatattaa tagaaataaa
    11161 gacattgacg catcccgccc ggctaactat gaattagatg aagtaaaatt tattaatagt
    11221 tgtaaaacag gagtttcatt acaatttata tatttaaaga ggcgaatgat tatgactgaa
    11281 atcgttgcag ataaaacggt agaagtagtt aaaaacgcaa tcgaaactgc agatggagca
    11341 ttagatcttt ataataaata tctcgatcag gtcatcccct ggcagacctt tgatgaaacc
    11401 ataaaagagt taagtcgctt taaacaggag tattcacagg cagcctccgt tttagtcggc
    11461 gatattaaaa ccttacttat ggatagccag gataagtatt ttgaagcaac ccaaacagtg
    11521 tatgaatggt gtggtgttgc gacgcaattg ctcgcagcgt atatttttct atttgatgag
    11581 tacaatgaga agaaagcatc cgcccagaaa gacattctca ttaaggtact ggatgacggc
    11641 atcacgaagc tgaatgaagc gcaaaaatcc ctgctggtaa gctcacaaag tttcaacaac
    11701 gcttccggga aactgctggc gttagatagc cagttaacca atgatttttc agaaaaaagc
    11761 agcttttcca gtcacaggta gataaaatca ggaaggaagc gtatgccggt gccgcagccg
    11821 gtgtcgtcgc cggtccattt ggattaatca tttcctattc tattgctgcg ggcgtagttg
    11881 aagggaaact gattccagaa ttgaagaaca agttaaaatc tgtgcagaat ttctttacca
    11941 ccctgtctaa cacggttaaa caagcgaata aagatatcga tgccgccaaa ttgaaattaa
    12001 ccaccgaaat agccgccatc ggggagataa aaacggaaac tgaaacaacc agattctatg
    12061 ttgattatga tgatttaatg ctttctttgc taaaagaagc ggcaaaaaaa atgattaaca
    12121 cctgtaatga gtatcagaaa agacacggta aaaagacact ctttgaggta cctgaagtct
    12181 gataagcgat tattctctcc atgtactcaa ggtataaggt ttatcacatt taaaaacaca
    12241 gacgtttttt aaagattgag agttacctgt tgataataag gtatcgggta actctcatta
    12301 tgacagagcg ctttcaaaaa cagggctacg aaatggcgag catccagacg tatattaaga
    12361 aagcccccat caacatctat taaatttatc actgcgccca gtcaggttta tttaagatat
    12421 gatcctgcca gtcaatcact tctgattctt tcactgcgat atgacgcaca gagatacgtt
    12481 caccatgcat tgccgctttc gaacccgtaa gtagcggatg ccacgcaggt aaatctttac
    12541 cttccaccag caaccgataa gcgcaggtca ttggcagcca ttcgaatgtt ggcagatttt
    12601 cacgggttaa tttaatgcag tcgggttcaa actcgaaacg acgttcgtag ttccgacatt
    12661 gacaggtttt aatattgagc tggcgacagg cgacgttagt gaagtagatt tcgtcggtgt
    12721 cttcatccat cagtttatgc aggcaacact gaccgcaacc atcacacaac gactcccatt
    12781 ccgcatcgct catttcgtcc agggttttac tttgccagaa aggtacatcg ctcatcaggt
    12841 gttccgccat tacgttaaaa cgcaccttat aaccagtctg gcacagcgat gcaagttttg
    12901 ccgccgcttt caggcggcaa aaagtattac aaaacgcgag ttgccaaaga atgcccatcg
    12961 aaagtgactg tcagctcatc accgctttgc aacgggccga cgccatcagg cgtgcctgtc
    13021 agcacaacgt caccggcctt gagggtaaaa aacttgctca tataagcgat cagcggaaca
    13081 attttatgga tcatgtccgc agtcgtacct tgctgacgtt gctcgccgtt tacgctcagg
    13141 cccagcgttg tattttgcgg gtcgccggta aattccgccg cgggaataaa cccggaaagc
    13201 ggacaagagt tatcaaacgc tttagccttt tcccacggct gcccggcttt cttcattttt
    13261 ccctgaacat cacgcaatgt cagatcgagc gccacgccat aaccggcaat ggctttgcgg
    13321 acatgctctt ccgtagcctg acgcagcgtc gcgccaatca acaccgccag ttcgacttca
    13381 tgatgaactg aaccgaaatc ggatgggatc gccaatggct gccgcagatc gcacagtgcc
    13441 gtttctggtt taataaacag cactggctct tcgggcactg cgctgcccat ctctttaata
    13501 tgtttggcat agttactgcc aacacagact actttactca ccggataatc cagcagcgca
    13561 ccttgccagt tgtgatgttg atacataacg ttcccctcac cttatcgtgg atatgaaaac
    13621 ccgcgccgac aaagataatg ccaccgccgg atatcggtta tttgttagtg tcgtctgttt
    13681 tctgccccat gacggaaaga tgttgcttca gcaaatcttc gggtggtggc ggtaactgca
    13741 aatagtaacc ttgctcggtt aatgccagtt taaccttttc aatatcagca ttgaccagtt
    13801 tcttccgccc atccaacggc agaatcatcg ctaactgagg ctgaccaaaa cctttcatca
    13861 gttcctcagg aacacgcgaa aaatcgtctt ttttttcgac atataaatag gtctggtcac
    13921 gcttgctgct tcgataaatc acacaaaaca tacttttact ctttaagatg ccaggttttg
    13981 gcataagcta acatcaatga aacccgcgcc acaagccagc cagctgtaga tttctacggt
    14041 ttgttgagtc catgcccctt tataagtaat ccccccggca aaaatgccgc gccgggattt
    14101 taccgaacgc aggaaagcca ctggaattct cccgttaggg attgttatat tataacagtt
    14161 catcgtactc attctgaaca ggagactacc aatgaaaatc aagagcatca gtaaggctgt
    14221 attgctgttt gcactgctaa cctccacgag ctttgctgcg ggtaagaatg tcaatgttga
    14281 gttcagaaaa ggccatagca gcgctcagta ctccggcgaa ataaagggat acgattacga
    14341 tacatatacc ttctatgcca aaaaaggtca gaaagtacat gtgagtatct ctaatgaagg
    14401 cgctgatact tacctcttcg ggccaggaat cgatgattct gttgatttat ccagatattc
    14461 cccagaactc gatagtcacg gacaatactc actccctgct tctggcaaat atgagctgcg
    14521 agtgctgcaa acccgaaacg acgcccgtaa aaacaaaact aaaaaatata acgtcgatat
    14581 tcaaattaag taaatgtcaa ccgacgggga ctctccccgt cataaacgtt aacgccccac
    14641 tcctctctgt cgctcaagag gttaatgacg aatagcagac aatgccgaat agaacataat
    14701 gtaaacaagg catcactcac gacacataaa taacatcttt gtggtgattt gattgatttt
    14761 tccactccgc ttaccatcca caccaaaata acgatcatca cgacataatt tttctttaac
    14821 atcgcaaaat acaccgttag cgaatgtaaa ttcgctggga tcgtagcctt gtaatgattg
    14881 taagttttca gcggcttttt cgccgagata tttccttgtt aactctgggg aaatgccatc
    14941 tgagttcaca caaatatacg ggtcgcaaac aacgccgcac gccggagatt gaagtccgcc
    15001 agcgtcatgt ccagacgcca ttgatgagaa catcatcccc acacctgaca atccgatata
    15061 aaaaatacgc atcatattca tagcacatcc tgataatcca accgtacaac acttatgctc
    15121 atttcatcat caatccgtaa tcaaaaccgc tgacctggac gagttacgat gacctgacag
    15181 aagtatcaga cgtcttctgc ggtttctatg atagagcaat tctgtggcgg atattaagat
    15241 aacgggtatc tgttctttaa tgatgccgca aaaaatggaa agttaagaaa gcctggcgtg
    15301 gtgaaaaaat acacttgcct caatactgga tatttaaaca ggtctggaga tgctgtcacg
    15361 attagggtgc ggaaaaagga agcgtaaata agaacaatca aaagtacgac ggcaatgggt
    15421 tgattgacaa gggtattttt taagctatga atcagcgcca tttatcacag aatagacttt
    15481 tactctgaat aaatgggagg gtgacttgcc tcaatataat ccagactata acatgcctta
    15541 tagtcttcgg aacatcatcg cgcgctggcg atgattaata gctaattgag taaggccagg
    15601 atgtcaaaca cgccaatcga gcttaaaggc agtagcttca ctttatctgt ggttcatctg
    15661 catgaggcag aacctaaggt tatccatcag gcgctggaag acaaaatcgc tcaggccccc
    15721 gcatttttaa aacatgcccc cgttgtactc aacgtcagtg cactggaaga cccggtaaac
    15781 tggtcagcga tgcataaggc ggtttcggca accggtttgc gggttattgg cgtaagtggc
    15841 tgcaaagatg cgcaacttaa agccgaaatt gaaaagatgg ggctgcctat cctgacggaa
    15901 ggaaaggaaa aagcgccgcg tccagctccc gcaccacagg ctccagcgca aaatacaacg
    15961 ccggtcacaa aaacgcgttt aatagatacc ccggtgcgtt ccggtcagcg tatttatgct
    16021 ccacaatgtg atctgattgt tacaagccac gttagcgctg gggccgaatt gattgccgat
    16081 gggaacattc atgtctatgg catgatgcgc ggtcgtgcgc ttgcaggggc aagtggtgac
    16141 cgggaaacgc aaatattttg tacgaacctg atggcggaac tggtgtccat cgcaggtgaa
    16201 tactggctga gtgatcaaat cccagcagaa ttttatggca aagcggcgcg actgcagtta
    16261 gtcgaaaacg ctttgaccgt tcaaccgtta aattgatccc tttttaacaa ggaatttcta
    16321 tggcacgcat tattgttgtt acttcgggca aagggggtgt tggtaagaca acctccagcg
    16381 cggccatcgc cactggtttg gcccagaagg gaaagaaaac tgtcgtgata gattttgata
    16441 tcggcctgcg taatctcgac ctgattatgg gttgcgaacg ccgggtcgtt tacgatttcg
    16501 tcaacgtcat tcagggcgat gcaacgctaa atcaggcgtt aattaaagat aagcgtactg
    16561 aaaatctcta tattcttccg gcatcgcaaa cacgcgataa agatgccctc acccgtgaag
    16621 gggtcgccaa agttcttgat gatctgaaag cgatggattt tgaatttatc gtttgtgact
    16681 ccccggcagg gattgaaacc ggtgcgttaa tggcactcta ttttgcagac gaagccatta
    16741 ttaccaccaa cccggaagtc tcctcagtac gcgactctga ccgtatttta ggcattctgg
    16801 cgtcgaaatc acgccgcgca gaaaatggcg aagagcctat taaagagcac ctgctgttaa
    16861 cgcgctataa cccaggccgc gtaagcagag gtgacatgct gagcatggaa gatgtgctgg
    16921 agatcctgcg catcaaactc gtcggcgtga tcccagagga tcaatcagta ttgcgcgcct
    16981 ctaaccaggg tgaaccggtc attctcgaca ttaacgccga tgcgggtaaa gcctacgcag
    17041 ataccgtaga acgtctgttg ggagaagaac gtcctttccg cttcattgaa gaagagaaga
    17101 aaggcttcct caaacgcttg ttcggaggat aagttatggc attactcgat ttctttctct
    17161 cgcggaagaa aaacacagcc aacattgcaa aagaacggct gcagattatt gttgctgaac
    17221 gccgtcgcag cgatgcagaa ccgcattatc tgccgcagtt gcgtaaagat attcttgagg
    17281 tcatttgtaa atacgtacaa attgatcctg agatggtaac cgtacagctt gagcaaaaag
    17341 atggcgatat ttctattctt gagctgaacg tgaccttacc ggaagcagaa gagctgaaat
    17401 aagcccgctg taaaaacgca tttatcttca aggcagagtc atttctgcct tgagtttttc
    17461 atccctctca tccacgttgt ggtaaagcgg cgagtattct tgctgatact cctcattgct
    17521 atttccacct ccccctttac accttaaggc tgttcccacg cacagaacaa aatgtttatg
    17581 cctggttgag taaataaccc tgttgttagt atggatatac tgggagatga taaccccgca
    17641 atttcattat tcattgttcc cccataggat gaggtttccc gccgtaactg gcgggctttt
    17701 tttgccccag aattttctct ttcaacatcc tgtaaacgaa aactgcgccg aagcgcagtt
    17761 taatcgttga tggtcagaac gaccagttca caccagccac cgcgttccac ggggactcca
    17821 caccggcacc atggctatac cccaccccga aatgcccgct taacgtactg ctgaatgagg
    17881 ctttaatacc tgcctggtat attccacgtc tgcccgacaa atcattgaca aaattaccgt
    17941 cactattcac tttcacccga ttatcatcga caaattcttt gcgcacagcc gccttcaggc
    18001 agggttcaat ttccataccg ttccccagac gcatgttgta actcagcgtt gcacccagtt
    18061 cacgatatat actgcgggta tcgactgatt tcgattccat gccattggat aaatgatagt
    18121 agcgtctaag tgacctaagc tctgctgcaa catcagcccg caggcaccag accaggggat
    18181 tcatcctgaa gagacagcgc aggtgtattg tgttcaccgc tcaccaaaga catcatgatg
    18241 aaatgatgat atcccgcata agaaagaggc attttttaca cgcagtgcgc tgaagtgtag
    18301 ttggataaaa aatcactctt ttcagataat actgtcgtac gcttatcttt cgacaatgag
    18361 gccgtcggca aaataaaatg actaatgtaa tcgttgctga tgaatatctt ctgctcacat
    18421 aaaaatcaca caataatatt gataaatcag attatcttac ctttacaaca ttgcgttaac
    18481 agtcaatgac acttcttgaa taaaaatatt gtcaatcaat gagttattcc tcatttaaga
    18541 ttattctcat tcacgtaaaa aacataattc ctatcatcac agccacgctc attttgaatt
    18601 atctgaataa tagcgagcgt tctttagtta agcagatctt aattgaagac gaaattattg
    18661 tgtgtgcaac ttacttaata ccggacatct aagttgaaga aaaaaatact tgccttcggg
    18721 cttatctcag cattgttctg ctccacgcca gcgatggcgg atatgaatcg caccacgaaa
    18781 ggtgcattgc taggcgcagg cgttggttta cttactggca atggtgttaa tggcgtactg
    18841 aaaggtgcag ctgttggcgc tggtgttggt gcagtaacag aaaaaggccg cgacggtaaa
    18901 aatgcgcgta aaggtgcaaa agtaggtgcc gccgttggtg ctgtcactgg cgttctgacc
    18961 ggcaatggcc tcgaaggggc gattaaaggt gccgtgattg gcggtacggg tggtgcaatt
    19021 ctgggtaaaa tgaaataagg aatgatgatg aaaaaatttg ctttactggc gggcctgttt
    19081 gttttcgcac caatgacctg ggcgcaggac tacaatatta aaaacggttt gccgtcagaa
    19141 acctatatta cttgcgcaga agccaacgaa atggcaaaaa ctgacagcgc acaggttgca
    19201 gaaattgttg cggtaatggg taatgccagc gttgccagcc gtgatttaaa aattgagcaa
    19261 tctccggaac tgagcgccaa agtggtagaa aaactgaatc aggtttgcgc taaagacccg
    19321 caaatgctgt tgatcacggc aattgatgac accatgcgtg ctatcggcaa aaaataatat
    19381 ttcatatgta ccgcgtcgat gaatacgacg cggtttttta gctaacggcg ggatcagaac
    19441 ttatatttca ggcccaccat cactgcggtg tcattgtagc cattatcacc cagctgcacg
    19501 cccacattcc cccagacgct ggccgctgga ttcaggttgc catttacgcc aagtttaatt
    19561 tcccccaaat tactcgcccc atcttgttcg actttcacac cgttcattga gacagcaaaa
    19621 tctttactgt tatggatcca gttcatttca acaaaaggtt caaagtcgtc accgtgcgga
    19681 ccgctgtttt tctcctgagt acgaataaag gtgcggaaac caagtcgggt ctggatgttg
    19741 ttatttgcgt cattctcaat acgtgagcca ttggcttcgg tgtgcgcatt atgatcgacg
    19801 cccatccaga tagcctgtgc ctgtggctgg aatatccacg tgtatttcgc cgcttccaga
    19861 ccaaaggatt tattcagggc aaagccgtaa cccgcttcca gcgagacggt tgcgcctttc
    19921 gcactatagg attcataggc taattcatcc cctttcaccg agtttttaaa ccagctatat
    19981 tgcgcccagg cgtcaatgta tgcgcctttt ttactgatgt catcggcaaa ccaaatggca
    20041 tacagccctg cgcaatagcc tctgacactc cctttcgaac gataatccga cacgctggaa
    20101 tgagttgagt tgtagtcgcg ggcataacca gccatcactc ccaaacgcca actgtcactg
    20161 tcggtaaaac caccggttaa cagctcggcc cctaactgcg agacgtagcg atgcgaggtc
    20221 gttctcaact gtccgttgct gtcacgccag gcattatgcc cgccaatttg acgtagccaa
    20281 agtcggcttg aacgctcctg ttcagttaca ggatcgatgt agcgcatttc acccgcacgg
    20341 tcgttcagac gcataacaaa aagagtgtta gcggctgcca ggttggcgac gtaacttcca
    20401 gcttcgaggc gcaggacggg ggtaggcgca acagtaggcg ctgaacttcc tccggagttt
    20461 gtttcaccgc cgctcgttgc ctggaagtta gtcagatacc agtttttatt ggtcgcttcg
    20521 gtattacctc gggttaatga atattcgtag gcaccggcga aaatcctcga atccttaata
    20581 aattccccat ttgattcccc ctcaacgctg ataatctcta tcccatttaa cgtgtaagct
    20641 ccgcggcctc ctgcattggt aactttaact cgggttgttc cagaggtatt tccattaata
    20701 acgactttat ccgtagctga attatcatca cccaattcag ttctgagatg caatacgcca
    20761 ttattaccaa cgtaattttc agttattgtt aatcttgttg gttcgacatc ccgtccatct
    20821 gcccgggaaa tatagaccgt actattatca accgtgaggt tgctcaccgt tgagtcagct
    20881 ttaatatccc aggtactatt atctgaaagc gacagatagg tatgagtgtt aacatctgtt
    20941 gaaatattag ctgaacccgt tatttttgaa ttagttgcat cgacggtcaa atgaccttta
    21001 atattattga taaaaacatc tgcagccgat gatacctcac tatccgtaat ggtcacgtta
    21061 ctcgtctcat ttttatggta gaggatttca tttgctgacc gtagtgagct accagataaa
    21121 atatctaccg ttgcttcctg accatttatg gaagactcca gcacaattcc agacccctga
    21181 tgcgcgactg cagatgtatt tttcagcgtc accgtgccgc cattataaat tttagcagtc
    21241 gcggcgtcag gacccgttgt ataaatttct ccctggttaa atgaaactgt cccctggcgg
    21301 gcagcgatgg catgagcatt gtccccatgc gtggtgatat caacatcggt taatgtaacg
    21361 ctggcattgg tataatcaag cgtgtcacca gccttgacga cagtgccatt agcgtacgcg
    21421 gcataacttt ctgttccgtg ggttgtgatc tccccttttg ccatatccag ttttgctatg
    21481 gcattatttt cattacttcc tgtatcggta gtactttctt catcaagaga agatacacac
    21541 gcacgcgcgc ttccgggaga tgcacacgca agaacgccga ctgcgtgatt acccgcagta
    21601 acaataaatg tatcatctcc gtcagaaaaa gagggggtaa ccgaaccgtc agagttgact
    21661 cttattatgg tgtcagtatt caggtttcca ctcgtttgac ttagcaaacc aattgagtaa
    21721 tcccccgttg tgacaatcgt gtttttaccc tgcaaaataa cagcaccgcc aaattcactt
    21781 acgattgcgc tagctgttaa tgctgtgctt agaggactat tgtcagaagg atataaaccg
    21841 cccgttgtta caatgacgcc attgttaatg gttaacttgc taccctctcc tgcaacggat
    21901 acccgtaact tccattaccc gttgttataa ctagcgcatt atcaatatct tttacttttc
    21961 catcttttat tgccacagca tgtttatatt catcgtgctg aataaactgt tcagcatatg
    22021 cattacccac cgccagagca ataagtccca aactaaaccc ggggagtttt ttcagtttca
    22081 tagattaacc atccattcat tggattaaca aacattcctg gtcaaataac ctatgagctc
    22141 agattaccaa aacggagggt aaagacaaaa acaaaagcac aaaaaattta aatagataaa
    22201 cagcgacaat atataaatga taaataagtc ggcattatca tttttcacct tattttcatg
    22261 acattgatca ctttgaggtg acgattttgt ttaacaaata actcttcgac atcacaatca
    22321 ttgatagttg caataaaaat tatttcaatg acatataaaa acaactgtga taacaccagg
    22381 gttgacaact atttgcgcaa taatgccttt ccttcaatga tccaggcgtt cactgccagc
    22441 aacccattca gccgcatact gacagtgaga aactgaaaga ttattgctga tccctgtggt
    22501 tgacgagtct ttctcttaat cgattaacca gattgatcgc cagccaaaca agggcattaa
    22561 gcaaatttat cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga
    22621 ccaggagggt gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca
    22681 gattattact attgttcatg ctactaatgg caatcctgtc aaaactttca aacaattata
    22741 gcaaacaaaa taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt
    22801 acaccgcact atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt
    22861 agaaaggata attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg
    22921 ctgaaatgtg atattatttt ggttcagata gtcaagttgt tctttcgttt cgacaccttc
    22981 agcgacgata ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt
    23041 tgattctggg tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat
    23101 atgcaggtca tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat
    23161 gacaaaacca ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa
    23221 tggctcacgc tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg
    23281 gaaatttaaa cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat
    23341 tccaatatga aaaccttccg gcaatttact cgcgatagcg ttcatctgtc tggcaacctg
    23401 attcatcagg ctttgcgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa
    23461 tgccgcgggt gatatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc
    23521 ccgcaatgtc ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt
    23581 atttattgcc cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag
    23641 caggaaggca gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc
    23701 gataagtctg tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt
    23761 tgtggagtga accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt
    23821 tggtccaata atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt
    23881 aagtgcccca cgaatatgct gatcgcttat cgtaaccaaa atgcgcgtat ctgcaaattg
    23941 gttctgatag agcaggatag gtaatctatt aacggtgtcg attgctggag ccaacagtaa
    24001 attactgtcc gggaaaacag gaatacgaga caacaggacc cgattcccag gcagggatgt
    24061 acaccagact attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc
    24121 ttcagtgcca agctgatatt gctcctcgag gttgcattcc ttcccggcaa tgttaaccgc
    24181 tgtttgagtc gcttgtgacg cttcttcaag tatatgattg atattattgg cggcatatct
    24241 cgctcccgcc aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac
    24301 accggtaata aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt
    24361 gtcccggcca aagggtttaa tacatgaaaa tgccattcat tcgctcgcgc tggcatctac
    24421 agagagcatg gtagagagca taagaaatat ccacgccgaa gataaaattt tctccatctt
    24481 gatctaaccc attaattatt gagcataatg taggcagtac aaaataagtt aggcgggata
    24541 tcaggcgtca agaatggaga gcgagaactc tccattcttg acacctgata ttgcggacat
    24601 aataagaaag cataacgcct gaaatgctca ctttgcatca gcatggtgat acagctgatg
    24661 tttattctaa aaccttactc aagttctaag agagcacgga ttccctgtca ttaataatga
    24721 tatccgttag tgtagtctta tgcatttcaa tgtactgcaa tatttgcaga tagagatcag
    24781 aaagaatcac actggtttca tcttttaaac gcaagcgtaa agcctgatca ataatatgac
    24841 tatcaacata cccatgcgtc atgaacacct ccctctcgag atttttaaac aaagcacatt
    24901 gaaaaataaa tctctctggg attgaattat tcatgtcagc ctcgtagaaa tattaaccca
    24961 ataaaattat gctgtgtata gtctaaagcg acagacaaaa ataagatgaa tgagttttct
    25021 ataaacttat ccttaataat tagaagttac atatcatcag ctgtgtatcg caacacgatt
    25081 tccagtgttt ttctaatcac atcagctttg agaatatcac ttgttgtttc cagggagtgt
    25141 atcagactta agataatatt tttattatta acattatcgc ctgaaagcat taaattggtg
    25201 acagcctgcc ctaacactgc tgactcttct tctaacaaat tacccgaact gcgaaagtaa
    25261 cttgctaacg ctttatcagt tattgcatta tgaattgtag tatcttcaag catggtaacc
    25321 tctcatctta cttatgaaat tttaatgtat tctgtttatt ttcttaccat tgaacatact
    25381 tttattgtca gctgtcgctg ttcttcggtt tggccctcct gaatcactat tgctaaaatc
    25441 acgaattatg ctgtcaatta aatcgcgtcg gaaagcatct tcctcagccg atttcattac
    25501 gagtaattgc ttaataagag tctgcaatga aaaatttatc ttcatgcgca gtatctggat
    25561 caccgcctgg ccagtaattt catattttat acgttcatta tcagatgtct tcataacgac
    25621 ttcatagcta acctgttaaa taatcaggct gttgcattat tcaaaaagca acccaattag
    25681 tgcattataa tgtttctgtt cctcttcccc agtcgcgtgt tccagtcggc aaagaagttt
    25741 ggcacaaagc gactttcgac tcagattacg tccatctttc aaaatctcag tcacaatttc
    25801 cccgagagtt tcctgctgag taggcaagtt agcttttgca aagtaacgag tgattgctcc
    25861 cgcagaatct aaagtcactg aattttgatg catgctacgc ctctgttaaa aatgttaatc
    25921 aatgttaagt gaggtaacaa tagcaaactt gtacgaaata tgtgtacaga atgataaatt
    25981 aattttttgc aattagtgct aatgcatata aaacaatgtg ttatgataaa acatattgca
    26041 tcatttaact gacaatctat acaagatttt tcgccaacta gtgttcaact ttttccattt
    26101 tttatcaatg taaagaaacg gttatgtttt ggtatcaaaa gaaagacaca agaagatatg
    26161 tctgttaccg tcttactctc gcctcaccca ttaccctgga ttgttaatgc ttaccaccct
    26221 tatttatcgt agccatatac gtgacgacga acctgtcaaa aaaatcgaag aaatggtttc
    26281 gatagcaaat cgcaggaaca tgcagtctga cgtaacaggg atcttactgt ttaatggttc
    26341 tcattttttc cagcttctgg aaggtccgga agaacaggtt aaaatgatat atcgggctat
    26401 atgccaggat ccacggcact ataatattgt tgagctgctg tgcgattacg cgcctgctcg
    26461 ccgttttggc aaagcgggaa tggaattatt tgatttgcgc ctgcacgagc gagatgacgt
    26521 tttacaggcc gtattcgaca aaggcacatc aaaatttcag ctaacttatg atgacagagc
    26581 actacaattt tttcgtactt ttgtccttgc aaccgaacaa tcaacctatt tcgagatccc
    26641 tgccgaagac tcctggcttt ttatcgctga cggatctgat aaagaacttg attcctgtgc
    26701 cctttcacca actataaacg accactttgc ctttcatcct attgtcgatc ccttatcgcg
    26761 gcggataatc gcttttgaaa ccattgtgca aaaaaatgaa gatagcccat cagccatagc
    26821 ggttgggcag cgtaaagacg gggaaatcta cacagcggat ctcaaaagta aggcgcttgc
    26881 attcacgatg gcacacgcac ttgagctcgg tgataaaatg atttcaatca atctattacc
    26941 tatgaccctg gttaacgaac ctgacgcagt ctctttttta cttaatgaaa taaaggccaa
    27001 tgctctggtg cctgaacaaa tcatcgttga atttactgaa agtgaagtca tatctcggtt
    27061 tgatgagttt gccgaagcga ttaaatcgct aaaggctgct ggtatcagtg tagcaattga
    27121 tcattttggc gcaggttttg ctggtttgtt actcctgtca cgcttccagc ctgacagaat
    27181 taaaatcagt caggaattga ttaccaatgt tcataaaagt gggccacggc aggcaattat
    27241 tcaggcgatc ataaaatgct gtacatcact tgaaattcaa gtcagtgcta tgggcgtggc
    27301 aacaccagaa gagtggatgt ggcttgaatc cgcaggaatt gagatgtttc agggagatct
    27361 gtttgctaaa gctaaattga atggtatccc ttccatagcg tggccagaga aaaaataatt
    27421 ttcagcacat tctttcacat gatttcagta aatcatcgtg aaaatgaata cgttacgttt
    27481 aaatttaatc cacattaaaa ataatgtttc atccaattta gctgaaatct tatttatact
    27541 ttgtacaatt ttagcgtata ttgtacaaag tat
//
LOCUS       DAEVZA010000058        27287 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEVZA010000058 DAEVZA010000000
VERSION     DAEVZA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.058"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1641
                     /locus_tag="LAF92_002503"
     CDS             <1..1641
                     /locus_tag="LAF92_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791494.1"
                     /translation="QQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEYSPAFFGEE
                     LFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPIAVRQQWRG
                     AMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADRGVDPLKLE
                     QELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQCPIKIDVP
                     EFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVRKLSEKHIG
                     MVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTSYYDAQVVA
                     DFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAKLGMPMVGV
                     DPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWYFFGHCTEV
                     TALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYELSWHQAMQ
                     RLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            1638..2048
                     /gene="menI"
                     /locus_tag="LAF92_002504"
     CDS             1638..2048
                     /gene="menI"
                     /locus_tag="LAF92_002504"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBI9791495.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            2148..2336
                     /locus_tag="LAF92_002505"
     CDS             2148..2336
                     /locus_tag="LAF92_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBI9791496.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            2884..3252
                     /gene="sufA"
                     /locus_tag="LAF92_002506"
     CDS             2884..3252
                     /gene="sufA"
                     /locus_tag="LAF92_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBI9791497.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            3261..4748
                     /gene="sufB"
                     /locus_tag="LAF92_002507"
     CDS             3261..4748
                     /gene="sufB"
                     /locus_tag="LAF92_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBI9791498.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            4758..5504
                     /gene="sufC"
                     /locus_tag="LAF92_002508"
     CDS             4758..5504
                     /gene="sufC"
                     /locus_tag="LAF92_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBI9791499.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            5479..6750
                     /gene="sufD"
                     /locus_tag="LAF92_002509"
     CDS             5479..6750
                     /gene="sufD"
                     /locus_tag="LAF92_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBI9791500.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            6747..7967
                     /gene="sufS"
                     /locus_tag="LAF92_002510"
     CDS             6747..7967
                     /gene="sufS"
                     /locus_tag="LAF92_002510"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBI9791501.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            7980..8396
                     /gene="sufE"
                     /locus_tag="LAF92_002511"
     CDS             7980..8396
                     /gene="sufE"
                     /locus_tag="LAF92_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBI9791502.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            8545..9549
                     /gene="ldtE"
                     /locus_tag="LAF92_002512"
     CDS             8545..9549
                     /gene="ldtE"
                     /locus_tag="LAF92_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBI9791503.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(9613..9849)
                     /gene="lpp"
                     /locus_tag="LAF92_002513"
     CDS             complement(9613..9849)
                     /gene="lpp"
                     /locus_tag="LAF92_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBI9791504.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(10160..11572)
                     /gene="pykF"
                     /locus_tag="LAF92_002514"
     CDS             complement(10160..11572)
                     /gene="pykF"
                     /locus_tag="LAF92_002514"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBI9791505.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            12129..12338
                     /gene="fumD"
                     /locus_tag="LAF92_002515"
     CDS             12129..12338
                     /gene="fumD"
                     /locus_tag="LAF92_002515"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBI9791506.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            12794..13420
                     /locus_tag="LAF92_002516"
     CDS             12794..13420
                     /locus_tag="LAF92_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBI9791507.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTANTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            13441..15543
                     /locus_tag="LAF92_002517"
     CDS             13441..15543
                     /locus_tag="LAF92_002517"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBI9791508.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            15556..16194
                     /locus_tag="LAF92_002518"
     CDS             15556..16194
                     /locus_tag="LAF92_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBI9791509.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            16258..16926
                     /locus_tag="LAF92_002519"
     CDS             16258..16926
                     /locus_tag="LAF92_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBI9791510.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            16923..17708
                     /locus_tag="LAF92_002520"
     CDS             16923..17708
                     /locus_tag="LAF92_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBI9791511.1"
                     /translation="MNPSQYAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            17712..18524
                     /locus_tag="LAF92_002521"
     CDS             17712..18524
                     /locus_tag="LAF92_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791512.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(18536..20140)
                     /locus_tag="LAF92_002522"
     CDS             complement(18536..20140)
                     /locus_tag="LAF92_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBI9791513.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTENNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(20266..20571)
                     /locus_tag="LAF92_002523"
     CDS             complement(20266..20571)
                     /locus_tag="LAF92_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBI9791514.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(20679..20755)
                     /locus_tag="LAF92_002524"
     tRNA            complement(20679..20755)
                     /locus_tag="LAF92_002524"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20719..20721),aa:Val,seq:gac)
     gene            complement(20760..20836)
                     /locus_tag="LAF92_002525"
     tRNA            complement(20760..20836)
                     /locus_tag="LAF92_002525"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20800..20802),aa:Val,seq:gac)
     gene            21144..22400
                     /locus_tag="LAF92_002526"
     CDS             21144..22400
                     /locus_tag="LAF92_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBI9791515.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDSASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFGVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(22441..23814)
                     /gene="mdtK"
                     /locus_tag="LAF92_002527"
     CDS             complement(22441..23814)
                     /gene="mdtK"
                     /locus_tag="LAF92_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBI9791516.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            24029..24670
                     /gene="ribE"
                     /locus_tag="LAF92_002528"
     CDS             24029..24670
                     /gene="ribE"
                     /locus_tag="LAF92_002528"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBI9791517.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(24710..25858)
                     /gene="cfa"
                     /locus_tag="LAF92_002529"
     CDS             complement(24710..25858)
                     /gene="cfa"
                     /locus_tag="LAF92_002529"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBI9791518.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(26149..>27287)
                     /locus_tag="LAF92_002530"
     CDS             complement(26149..>27287)
                     /locus_tag="LAF92_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBI9791519.1"
                     /translation="LPAFAAIQADLQTPASAVSASLSLFLAGFAAAQLLWGPLSDRYG
                     RKPVLLIGLTIFALGSLGMLWVENAATLLVLRFVQAVGVCAAAVIWQALVTDYYPSQK
                     VNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQAIFATLFAITVVLILPIFWLKPTTK
                     ARNNSQDGLTFTDLLRSKTYRGNVLIYAACSASFFAWLTGSPFILSEMGYSPAVIGLS
                     YVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLVLFAVSVIATWAAGFISHVSLVEILI
                     PFCVMAIANGAIYPIVVAQALRPFPHATGRAAALQNTLQLGLCFLASLVVSWLISIST
                     PLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNHGNAEVAHSESH"
BASE COUNT         7107 a         6387 c         7122 g         6671 t
ORIGIN      
        1 caacaagaga ttttgatgaa gcaaatctct gatgacgtag tggcgctgac tgcgaaatac
       61 ggtggtttgt tgtggggcga gcatggcaaa ggttttcgcg ctgaatacag cccggcgttt
      121 ttcggtgagg aactttttgc agaactgcgc aaagtgaaag cggcatttga cccgcataac
      181 cgactcaacc cagggaagat ttgcccgcca gaaggtctcg atgcgccgat gatgaaagtg
      241 gacgcggtga agcgcggtac attcgatcgg cagatcccca ttgcggtacg ccagcagtgg
      301 cgcggtgcga tggagtgtaa cggcaacggt ttatgcttca actttgatgc ccgtagtccg
      361 atgtgtccgt cgatgaagat cacccagaac cggattcatt caccgaaagg gcgcgcaacg
      421 ctggtgcgtg aatggctgcg tttgttggcg gatcgcggcg ttgatccact caaactggaa
      481 caagaactgc ctgaatccgg cgtcagtttg cggacgttaa ttgcccgcac gcgcaatagc
      541 tggcatgcga ataaaggcga atatgacttc tcacacgaag tcaaagaggc gatgtcgggc
      601 tgtctggcct gtaaagcgtg ttcgacccag tgccccatca aaattgatgt gccggagttt
      661 cgctctcgtt ttctgcagct ctatcacacc cgttatttac gcccgctgcg cgaccacctc
      721 gtcgctacgg tcgagagcta cgcgccgctg atggcacgcg cgccgaaaac ctttaacttc
      781 ttcattaacc agccgctggt gcgcaaactc tcggaaaaac atatcggcat ggttgatttg
      841 ccgctgctgt cggtcccctc gctacaacaa caaatggtgg gtcatcgctc ggcaaacatg
      901 acgctggaac agcttgaatc tctcaatgca gagcagaaag cgcgcacagt gttggtggtg
      961 caggacccct ttaccagcta ttacgatgcg caagtggtgg cggattttgt ccgtctggtc
     1021 gaaaaattag gtttccagcc tgtgttactg ccattttcgc caaatggcaa agcccagcat
     1081 attaaaggtt ttcttaatcg ttttgcgaag acggcgaaaa agacggcgga tttcctcaac
     1141 cgtatggcga agctgggtat gccaatggtg ggcgtcgatc cggcgctggt actttgttat
     1201 cgcgatgaat ataaactggc gctgggcgag gaacgtggcg agtttaacgt cttactggcg
     1261 aatgaatggc tggcaagcgc acttgagtca cagccagtgg ctacagtcag cggtgaatca
     1321 tggtatttct ttggtcactg taccgaagtt accgccttgc cgggtgcgcc agcacaatgg
     1381 gccgcgatat ttgcccgttt tggcgcgaaa ctggaaaatg tcagcgtggg ttgctgcggc
     1441 atggcaggga cttacggaca tgaagcgaaa aaccataaaa attcgctcgg gatctatgag
     1501 ttatcctggc atcaggctat gcagcgactg ccgcgtaacc gctgtctggc gaccggatat
     1561 tcctgccgta gccaggtaaa acgggttgaa ggcacggggg tacgccatcc tgtgcaggct
     1621 ttactggaga ttattaaatg atatggaaac ggaaaatcac cctggaagca ctgaatgcta
     1681 tgggtgaagg aaacatggtg gggttcctgg atattcgctt tgaacatatt ggtgatgaca
     1741 cccttgaagc gacaatgcca gtagactcgc ggacaaagca gcctttcggg ttgctgcatg
     1801 gaggcgcatc cgtggtactg gccgaaagta tcggttccgt tgccggttat ttatgtaccg
     1861 aaggtgagca aaaagtggtt ggtctggaaa tcaatgctaa ccacgtccgc tcggcacgag
     1921 aagggcgggt gcgcggggta tgcaaaccgt tgcatctcgg ttcgcgtcac caggtctggc
     1981 agattgaaat cttcgatgag aaagggcgtt tgtgctgttc gtcacgattg acgaccgcca
     2041 ttttgtgacg ttgtcaccag aaaagtgtga cgcatattgc ttttggtcat tatctgcaca
     2101 gtaaacggtg gtatactgtg caggtcttgt ctaaaagcga ggatgctatg tctactcaac
     2161 ttgatcccac ccagctggcc attgaatttt tacgccgtga ccagagcaat ctttcacctg
     2221 cccaatatct caaacgtctg aaacaactag aacttgaatt tgctgacctg cttactctct
     2281 cttctgctga attaaaagaa gaaatctact ttgcctggcg tctgggcgtg cattgatcct
     2341 gacttaatgc cgcaagttcg ccaaatcgac ctggattaac ctccgtccag gttcgatgac
     2401 cttcttaata ctgcttatct accgcctgat attgttcaaa aataatacta atcgcagttt
     2461 gtgttaaaac ggcgggttag ctttatgaga ttattcttat cgccccttca agagctaagc
     2521 cactgagagt gccggagata agcgccggat ggggtagaaa cccttaagcc tgtgttgcac
     2581 agacttaagg gttttcttat ttctgaacgc tgcacttttt tacctatctc ttaaatagtc
     2641 attttcaata caactttttc tttttcatca cagccttaat gctccttaga taataaccat
     2701 tatctaacaa tgagatacct aattcttagc gtgcctgtta acccacacat cagggtctat
     2761 gcttattaaa agcaggcaaa aagcatgttt tacggtaaag cccctgcgtt tgctgggttg
     2821 tactgataat cattatcact aacatgctgt tatacgctga aagcgatgaa gtgaggtaaa
     2881 tcgatggaca tgcattcagg aacctttaac ccacaagatt tcgcctggca aggcttaacg
     2941 ctgacacccg cagcggcgat acacatccgt gagctggtgg caaagcagcc gggtatggtc
     3001 ggcgtgcgct taggcgtgaa gcaaacgggc tgcgcgggct ttggctatgt gctcgacagt
     3061 gttagcgagc cggacaaaga cgatctgctg tttgaacacg acggcgcgaa gctgtttgtc
     3121 ccgctgcaag cgatgccgtt tattgatggc acggaagtcg atttcgttcg tgaaggactt
     3181 aatcagatat tcaaatttca caaccctaaa gcccagaatg aatgtggctg tggcgaaagc
     3241 tttggggtat aggcggtact atgtctcgta atactgaagc aactgacgat gtcaaaacct
     3301 ggaccggcgg cccgctgaat tataaagaag gattcttcac ccagttagcc accgatgagc
     3361 tggcaaaggg gataaacgaa gaggtggtgc gcgcaatttc ggcgaagcgt aatgagccgg
     3421 agtggatgct gaagtttcgt ctaaacgcct atcgcgcatg gctggagatg gaagaaccgc
     3481 actggttgaa agcgcactac gacaagctga attatcagga ttacagctac tactcagcac
     3541 catcgtgcgg taattgtgac gacacttgcg cgtctgaacc tggcgcggtg cagcaaactg
     3601 gcgcgaacgc ctttttaagt aaagaggtgg aggcggcgtt tgagcagttg ggcgttcccg
     3661 tgcgggaagg caaagaggtg gcggtggatg ccattttcga ctcagtttcg gttgccacta
     3721 cttatcgcga aaaactggcg gagcagggaa ttattttctg ttcctttggt gaggcgatcc
     3781 acgatcaccc ggaactggtg cgtaaatatc tcggcaccgt ggtgccgggg aatgacaact
     3841 tctttgccgc gcttaatgcg gcggtagcct ctgacggtac gtttatttat gtgcctaaag
     3901 gcgtgcgctg cccgatggaa ctttccacct attttcgcat taacgcggaa aaaaccgggc
     3961 agtttgagcg caccattctg gtggccgacg aagacagcta cgtcagctac attgaaggct
     4021 gttccgctcc ggtgcgtgac agctatcagt tacacgcggc agtggtggaa gtcatcatcc
     4081 ataaaaacgc cgaggtgaaa tattccacgg tacaaaactg gttccctggc gataacaaca
     4141 ccggcggtat tctcaacttc gtcaccaagc gtgctttgtg cgaaggcgaa aacagcaaaa
     4201 tgtcatggac gcaatcagaa accgggtccg cgattacgtg gaaatatccc agctgcattt
     4261 tgcgcggcga taactccatt ggtgagtttt actcagtggc gctgaccagc ggtcatcagc
     4321 aagcggatac cggcaccaag atgatccaca tcggtaaaaa caccaaatcg accattatct
     4381 cgaaagggat ctctgccgga catagtcaga acagttatcg cggcttagtg aaaatcatgc
     4441 cgacggcaac caatgcgcgc aatttcactc agtgcgactc aatgctgatt ggcgctaatt
     4501 gtggggcgca taccttcccg tatgtcgaat gtcgcaataa cagcgcacaa ctggagcacg
     4561 aggcaacgac atcacgtatt ggtgaagatc aactgtttta ctgcctgcaa cgcgggatca
     4621 gcgaagaaga cgccatctcg atgattgtta acggtttctg caaagacgtg ttctcagaac
     4681 tgccgctgga atttgccgtt gaagcacaaa aactcctcgc catcagtctt gaacacagcg
     4741 tcggataagg aataaacatg ttaagtatta aagatttaca cgtcagcgtg gaagataaag
     4801 ctatcctgcg tggattaagc ctcgccgttc gtcccggcga agttcacgcc attatggggc
     4861 caaacggttc gggcaaaagt accttatcgg caacgcttgc cgggcgcgaa gattatgaag
     4921 tgacgggcgg cacggttgag ttcaaaggca aagatttgct tgcactgtcg ccggaagatc
     4981 gtgcaggcga aggcatcttt atggccttcc agtatccggt ggagattcca ggcgtcagta
     5041 accagttttt cctgcaaacg gcacttaatg cagtgcgcag ctatcgcggc caggaaacgc
     5101 tcgaccgctt tgattttcag gatttgatgg aagagaaaat cgctctcctg aagatgccgg
     5161 aagatttatt aacccgttcg gtaaacgttg gtttttccgg cggcgagaaa aagcgcaacg
     5221 atattttgca aatggcggtg ctggaaccgg agttatgcat tcttgatgag tcggactccg
     5281 ggctggatat tgacgcatta aaagtggtcg ccgatggcgt gaactcgctg cgtgatggca
     5341 agcgctcatt catcattgtt acgcactacc aacgcattct cgactacatc aagcctgatt
     5401 acgtccatgt gctatatcag ggacgaattg tgaaatccgg cgatttcacg ttggtcaaac
     5461 aactggagga gcagggttat ggctggctta ccgaacagca gtaacgcgct gcaacagtgg
     5521 catcacttgt ttgaagctga agggacaaaa cgctcaccgc aagcacagca gcatttacaa
     5581 caattgctgc gtaccggact gccgacacgt aaacatgaaa actggaaata tacgccgctg
     5641 gaagggctga ccaatagcca gtttgtcagc attgcgggag agatatcccc acagcagcga
     5701 gatgccttag cgttaacgtt agacgctgtg cggctggtat ttgtcgatgg acgttacgtg
     5761 tcagcactga gcgatgcgac tgaaggcagc gggtatgaag tgagcattaa cgacgaccgt
     5821 cagggggtac ccgacgctat tcaggcggaa gtgtttctgc atttgacgga aagcctggca
     5881 caaagcgtga cgcatatcgc cgtgaagcgc ggtcaacggc cggcaaagcc attgctgtta
     5941 atgcatatta cccagggcgt ggcaggtgaa gaggtcaaca ctgcccatta ccgacatcat
     6001 ctggatctgg cggaaggtgc cgaagcaacg gtgatcgaac attttgtcag ccttaatgat
     6061 gctcgtcact tcaccggcgc acggttcact atcaatgtcg cagcgaacgc ccacttgcag
     6121 catatcaagc tggcgtttga aaacccggtc agtcaccact ttgcccataa cgatttgttg
     6181 ctggctgacg atgccaccgc atttagccat agtttcctgc tgggtggcgc agtgttacga
     6241 cacaacacca gtacgcaact caatggcgaa aacagcacgc tgcggatcaa tagcctggcg
     6301 atgccggtga aaaacgaggt gtgtgatacc cgtacctggc tggaacacaa taaaggtttt
     6361 tgtaacagcc gacagttgca caaaactatc gtcagcgaca aaggccgcgc ggtatttaac
     6421 ggtttgatca acgtcgcgca gcacgccatc aaaacggatg gtcagatgac caacaataat
     6481 ctgctgatgg gcaaactggc agaagtggat acgaaaccgc agctggaaat ctatgcagat
     6541 gatgtgaaat gcagccacgg cgcgacggtg gggcgtattg atgatgaaca gatgttctat
     6601 ctgcgctcgc gcgggatcaa tcagcaggat gcccagcaga tgatcattta cgcctttgct
     6661 gccgaactga cggaagcact gcgtgatgag gggcttaaac agcaggtgct ggcccgaatc
     6721 ggtcaacgac tgccaggagg tgcaagatga ctttttccgt cgacaaagtg cgggccgact
     6781 ttccggtgct ttcgcgtgag gtaaacggtt tgccgctggc ttatctcgac agcgccgcca
     6841 gtgcgcagaa accaagccag gtgattgact ccgaagccga gttttatcgt cacggctacg
     6901 cggcggtgca tcgcggtatt cataccttaa gcgcccaggc gaccgagaaa atggagaacg
     6961 tgcgcaagcg ggcatcgctg tttattaatg cccgttcggc ggaagagctg gtgttcgtcc
     7021 gcggcacgac ggaagggatc aatctggtcg ccaatagttg gggcaacagc aacgtgcggg
     7081 cgggcgataa catcatcatc agtcagatgg agcaccacgc taacattgtt ccctggcaga
     7141 tgctttgcgc acgcgttggc gcagagctgc gtgtgatccc gctcaatccc gacggtacgc
     7201 tgcaactgga gacgctacct aatctgttcg atgagaaaac tcgcctgctg gcaattactc
     7261 atgtctccaa cgtgcttggc acagaaaatc ccctggcgga aatgatcacg cttgcgcatc
     7321 agcatggcgc aaaagtgctg gtggatggcg ctcaggcggt gatgcatcat ccggtggacg
     7381 ttcaggcgct ggattgcgat ttttacgtat tctccgggca taaactgtat ggccccaccg
     7441 gaattggcat tctttatgtg aaagaagcct tgttgcagga gatgccgccg tgggaagggg
     7501 gcggttctat gatcgccacc gtcagcctga gtgaaggcac tacctggacc aaagcaccat
     7561 ggcggtttga agccggtaca cccaataccg ggggcatcat tggtcttggc gcggcgctgg
     7621 agtatgtttc ggcgctgggg cttaataaca tagccgagta tgaacagaat ctgatgcact
     7681 acgcgctatc acagctggaa tctgtaccgg atctcactct ctatggcccg cggaacaggc
     7741 ttggcgttat tgcttttaat cttggtaaac accacgccta tgatgttggc agttttctcg
     7801 ataactacgg cattgctgtg cgtaccggac atcactgcgc tatgccatta atggcctatt
     7861 acaacgtccc tgctatgtgt cgggcgtcgc tggccatgta taacacccat gaagaagtgg
     7921 atcgtctggt gaccggactg caacgtattc accgtctgct gggataacag ggaggcacta
     7981 tggctttatt gccggataaa gaaaaattgc tgcgtaattt tttacgctgc gccaactggg
     8041 aagagaaata tctctacatt attgagctgg gccagcgtct gccagaatta cgcgccgaag
     8101 accgaagccc acaaaatagc attcagggct gccagagtca ggtgtggatt gtcatgcgcc
     8161 agaatgcgca gggaattatt gaattgcagg gcgacagcga tgcggcgatt gtgaaagggc
     8221 ttattgcggt cgtctttatt ctctacgatc agatgacgcc gcaggatatt gtcaatttcg
     8281 atgtgcgtcc gtggtttgaa aaaatggcgc tcacccaaca tctcacccca tctcgttcac
     8341 aaggtctgga agcgatgatt cgcgcaattc gcgccaaagc cgctgcactt agctaaacta
     8401 aagggacagc tttcatccgg ttggctcaca ttgccggatg gtcaatgttt acactgacct
     8461 gcacaggagt gttttggttt caggtgaaca taagcgccat ccgacgtttc agcgtgagtc
     8521 tccggcaaat acaggaggtt tacaatgaaa cgcgcgtctt tgcttacact cacgcttatc
     8581 ggcgctttta gcgccatcca ggctgcctgg gcggttgatt atccgctacc accaaccgga
     8641 agccgactgg ttgggcaaaa tcaaacgtat acggtgcaag aaggggataa aaaccttcag
     8701 gctattgccc gacgttttga tactgcggca atgttgatcc ttgaagccaa taacactatc
     8761 gccccggtgc caaaacctgg tacgacgata actattcctt cacaactgtt attacctgat
     8821 gcaccgcgtc aggggattat cgttaacctt gcagagctgc gcctttatta ttatccgccg
     8881 ggagaaaata ttgtgcaggt ttatccaata ggtattggat tgcaggggct ggaaacgccg
     8941 gtgatggaaa cgcgtgttgg gcagaaaatc cctaacccaa cctggacgcc tacggcaggc
     9001 attcgtcaac gttcgctgga gcgtggcatt aaattaccgc cagtcgttcc tgccggacca
     9061 aataacccgc taggacgtta cgcactgcgc ctcgcgcatg gtaatggcga atacctcatt
     9121 catggtacca gtgcgccgga cagcgtcggt ttgcgcgtca gttcagggtg tattcgcatg
     9181 aatgcaccgg atattaaagc cttgttctcc agcgtgcgga cgggaacgcc ggtgaaagtg
     9241 atcaacgaac cggtgaaata ttccgtggaa cctaacggga tgcgttatgt tgaagtacat
     9301 cgaccactat cagcagaaga acagcaaaac gttcagacaa tgccatacac actgccagca
     9361 ggctttacgc aatttaaaga caataaggct gtagatcaga agttagtcga taaagcgttg
     9421 tatcgtcggg cagggtatcc ggtttcggtg agcagtggag caactcccgc agccagcaat
     9481 gcgccttcag tagagtcagc gcagaatggt gaaccagagc aagggaatat gttacgcgcg
     9541 acgcagtagc ggtaaacggc agacaaaaaa aatggcgcac aatgtgcgcc atttttcact
     9601 tcacaggtac tattacttgc ggtatttagt agccatgttg tccagacgct ggttagcacg
     9661 agctgcgtca tctttagcag cctgaacgtc ggaacgcatt gcgttcacgt cgttgctcag
     9721 ctggtcaact ttagcgttca gagtctgaac gtcagaagac agctgatcga ttttagcgtt
     9781 gctggagcaa cctgccagca gagtagaacc caggattacc gcgcccagta ccagtttagt
     9841 agctttcatt attaataccc tctagattga gttaatctcc atgtagcgtt acaagtatta
     9901 cacaaagttt tttatgttga gaatattttt ttgatgggaa ggcacttatt tttgatcgtt
     9961 cgctcaaaga agcatcgaac gctgggtttt agataaaaaa attgagagaa aacaggatgc
    10021 ttccatcgga ttcatcttag ataaagtgag attatatagt gaaatccgat ttgatttttt
    10081 attgcttctg gttatcgatt aaataaaaaa agcgcccatc agggcgcttc gatatacaaa
    10141 ttaattcaca aaagcaatat tacaggacgt gaacagatgc ggtgttagta gtgccgctcg
    10201 gtaccagtgc accagaaacc ataactacaa cgtcaccttt gtgtgccaga ccgctctgca
    10261 gagccagttc tttacccaga cggtagaaat catcagtaga agtgatctct ttaaccagct
    10321 gcggcacaac gcctttgctc agtaccaact gatgagccgt tttttcgttg gtggtcagtg
    10381 ccaggatggt ggcatccggg aagtatttac gtactgcgcg agcagattta ccgccctgag
    10441 tagcaaccac gatcagcgga gcatccagtt tttcagcagt ttcaacggca ccacggcata
    10501 ccgcttcggt aatgcgcagt ttacggttgt cattgttgaa ctcgagacgg ctgttcatca
    10561 cgcggtcggt acgttcgcaa atggtcgcca tgatagaaac cgcttccagc gggtatttac
    10621 cttttgcgga ttcaccagac agcatcactg catcagtacc gtcgaggatg gcgtttgcaa
    10681 cgtcaccggc ttctgcgcga gtcgggcgtg ggtttttgat catggaatcc agcatctggg
    10741 tcgcagtgat aacgacttta cgtgcacgga tacatttttc gatcatcatc ttctgggcga
    10801 agataacttc ttctaccggg atttctacac ccaggtcgcc acgcgcaacc atgatgccgt
    10861 cagaggcttc gaggatttcg tcgaagttgt tgaggccttc ctggttttcg attttggaga
    10921 tgatgtggat gttttcgccg ccgtgcgctt tcaggtgctc acggatttcg ataacgtcag
    10981 aacgcttacg aataaaggaa gcagcaacaa agtctacgcc ttgttcgcaa ccaaagatca
    11041 ggtcctgttt gtctttttca gccagtgctg gcagagcaat ggaaacgcca ggcaggttca
    11101 cacctttgtt ttcgcccagg tcaccgttgt tcagcacttt acagataact ttgttacctt
    11161 caatggcggt aacttccata ccgatcagac catcgtcaac cagtacggtg ttgccaactg
    11221 acaggtcagt agtgaaacct tcatacgtta ccgcaaccat ttcgctgttg ccgataacag
    11281 atttatcagt ggtgaaagta aaggtctgac cagctttcag agaaacgtcg ttaccgcctt
    11341 ccagtttcat ggtgcggatt tccggacctt tggtatcaag caggatagcg gcggttttac
    11401 cagttttgct catcacgttg cgcagattct gaatgcgctg accgtgttct gcatagtcac
    11461 catgagagaa gttcagacgc ataacgttca tgccagcgtc cagcatttta gctaacatct
    11521 cttcagattc ggttttcggt ccgatggtgc aaacaatttt ggtctttttc atgacagtct
    11581 tagtctttaa gttgagaagg atgggagaaa cttgctttct gggcgcactg ccggaaagcg
    11641 aatccggtat tacgaaagtg gtggtgcctc gctctaagga taggtgacat cgaaaaagcg
    11701 tgcagaggaa tgtgtgcttg tgtctcagcc caacgtgagt gcctgttttt gagtcgttga
    11761 gttctacagt ccaaagtgct gaaaccattc aagagtcaat tggcgcgcat tatacgctga
    11821 aattaatcca aaaggaaaac aaaaacagcg tttcaaaaaa tatacaactt gttagacaat
    11881 aaattgttat caaagattta acgttccagg gaaaaggtta cgcaaaaact tcgctgtttg
    11941 cgcaacgtct ggtgccaaag ttgtcagtgt gaggataagt gaaagttcat ccggtgtata
    12001 aatatatcat cgctcaattg ctatagttaa aataatttat cgcgttattt gtctgatttt
    12061 gtttgatttt tgtttaattg gttagattga tatatgctga tagttggcat gataaatagg
    12121 tataaagaat gggaaatcgg acaaaagagg atgaactgta ccgagaaatg tgcagagttg
    12181 tcggtaaagt tgtgctggaa atgcgcgatc tggggcagga accaaagcat attgttatcg
    12241 caggcgtatt acgtacagca ttagccaata aacgcattca gcgcagtgag ctggaaaagc
    12301 aggcgatgga aaccgtcatt aacgcactgg taaaatgacc attcaggatg aggcggtaag
    12361 cttcatcctg atattcttgc ttcagttgcg gtccagttaa tcattctaaa taacaactgc
    12421 tgaaattata cgaatcgcgg tatgtatttt aatttataac gataatatta tttttatttt
    12481 ctgcaatgaa cgtcacaaat aattgtcgct taagttaaat ttatttcaaa tgataataaa
    12541 tatcagttca cttataattt cttgctttgt aattttaatt gataacgatc aatgttaagg
    12601 gctcagaata atcacattat tttttctcct aaccataaag gattagttta tcggcgttaa
    12661 atcttttctc atacactatc gggttaggga aaatatctgg tgttggggac agagtcagca
    12721 gcgtattagt tgaatgctta atatcaggct gctgaattgt cagcactgtg tttgcgaaga
    12781 ggatgcgaaa taaatgaacc cggttgatcg tccactatta gatattggct taacgcgatt
    12841 agaattcttg cgtatatcag gaaaaggcct tgctggtcta acaattgccc ctgcgttact
    12901 gtcacttttg ggttgcaaac aggaagatat tgatagcggc acagtagggt tgataaatac
    12961 acccaaaggg gtgctggtga cccagcgagc gcgatgcacg ggttgccacc gctgtgaaat
    13021 ctcttgtacc aacttcaacg atggctcagt agggacattc ttctcccgta tcaaaatcca
    13081 tcgcaattat ttctttggcg acaacggggt tggctctggc ggcggcctgt atggcgatct
    13141 caactatacc gcgaacacct gccgtcaatg caaagagccg caatgcatga acgtctgccc
    13201 gattggtgcg attacctggc agcagaaaga aggctgtatt accgtcgatc ataaacgctg
    13261 tattggctgt agcgcctgta ctacggcatg cccgtggatg atggccaccg taaataccga
    13321 aagtaaaaaa tcctcgaaat gtgtgttatg cggtgaatgc gcaaacgcct gcccaacagg
    13381 ggcgttaaaa attatcgagt ggaaagatat tactgtgtga atcttgcaaa ggaaaaaatc
    13441 atggctaacg gttggacagg taatatatta agagtcaatc tcacgacagg aaatattacc
    13501 ctcgaagatt ccagtaagtt taaaagtttt gtcggtggca tgggcttcgg ctacaaaatt
    13561 atgtatgacg aagtaccgcc aggcacgaaa cctttcgatg aagcgaataa attagtcttt
    13621 gctaccggcc cattaactgg atctggtgcc ccctgtagtt ctcgcgtaaa tatcacctca
    13681 ctttctactt ttaccaaagg aaatttagtt gtcgatgccc atatgggtgg cttttttgca
    13741 gcgcaaatga aattcgctgg atacgacgtc attattatcg aagggaaggc gaaatcaccg
    13801 gtatggctga agattaaaga tgacaaagtt agcctggaaa aagccgattt cttatgggga
    13861 aaagggacgc gcgcaacgac ggaagaaatt tgtcgattga ccagtccgga aacctgtgtg
    13921 gcggctattg gtcaggctgg ggaaaacctt gttcctctct ctggcatgtt gaatagccgt
    13981 aaccacagcg gtggtgcggg aactggcgca ataatgggtt cgaaaaacct gaaagcgatt
    14041 gcggttgaag ggacgaaagg ggtcaacatt gccgatcgtc aggagatgaa gcgtctcaac
    14101 gattacatga tgactgaact tatcggtgcg aataacaacc atgtcgtgcc aagtacgccg
    14161 caatcgtggg cagagtattc ggatcccaag tcacgttgga cagcacgtaa agggctgttt
    14221 tggggcgcgg ctgaaggcgg tccaattgaa acgggggaaa ttccgccagg caatcagaat
    14281 acggtcggct ttcgtaccta taaatccgtt tttgacttag gtccggcggc agagaaatac
    14341 acagtaaaaa tgagcggctg ccactcttgc ccgatccgtt gtatgaccca aatgaatatt
    14401 cctcgggtca aagagtttgg cgtgcccagc acaggtggta acacttgtgt agcaaacttt
    14461 gtccatacca ccatcttccc gaacgggccg aaagattttg aagataaaga cgatggtcgt
    14521 gtgattggta acctggttgg tctgaatttg ttcgatgact acggcctatg gtgtaactac
    14581 gggcagctgc atcgcgactt tacttattgt tacagcaaag gtgtgttcaa gcgtgttctg
    14641 ccagctgaag agtatgcgga aattcgctgg gatcaactgg aagcgggtga cgttaacttc
    14701 attaaagatt tttactaccg tctggcgcat cgtgtgggtg agctgagtca cctggctgat
    14761 ggttcatatg ccatcgcaga acgctggaat ttgggtgaag agtactgggg ctacgcgaaa
    14821 aataaactct ggtcgccgtt tggctatccg gttcaccatg ccaatgaagc gtcagcgcag
    14881 gtcggttcca ttgttaactg tatgttcaac cgtgactgca tgacacatac ccatatcaac
    14941 tttattggtt ccggcttgcc attgaaactg caacgtgaag tggcgaaaga gctttttggt
    15001 tctgaagatg cctacgatga aaccaaaaac tacacgccaa tcaatgacgc aaaaattaaa
    15061 tatgctaagt ggtcgctgtt gcgggtctgt ttgcataacg ccgtcaccct gtgtaactgg
    15121 gtctggccaa tgaccgtttc gccgctgaaa agccgtaatt accggggcga tctggcgctt
    15181 gaagccaaat tcttcaaagc gatcaccggc gaagagatga ctcaggaaaa attagattta
    15241 gctgcagagc gtatctttac gttgcatcgt gcctacacgg taaaactgat gcaaaccaaa
    15301 gatatgcgta atgaacacga tcttatctgt tcctgggtat tcgacaagga tccgcagatc
    15361 ccggtcttta ctgaaggtac tgacaaaatg gatcgtgacg atatgcatgc ttcgctcacg
    15421 atgttctaca aagaaatggg ctgggaccca cagcttggtt gtccgacccg cgaaacattg
    15481 cagcgtctgg ggctggaaga tattgccgcc gatctggcag cacacaatct actgcctgtg
    15541 taaggaatgg gtgagatgaa ccatcgggat gaattgccgt tagcgaaagt cagcgaagtt
    15601 gacgaggcga agcggcaatg gttacagggg atgcgtcacc ccgttgatac ggtaaccgaa
    15661 ccagaaccgg ctgaaatcct ggcagagttc attcgccagc attctgtggc tgggcagctg
    15721 gtggcgcgcg ccgtgtttct ctcgccaccc tattcggttg cagaagagga gctgtcagtt
    15781 ttgctggaaa gcattaagca gaacggagac tacgcggata tcgcttgtat gacgggaagc
    15841 caggatgatt actattactc aacgcaggcc atgagtgaaa actacgcggc gatgtctttg
    15901 caagttgtgg agcaggacat ttgtcgcgcc attgcacacg ccgttcgttt tgagtgccag
    15961 acatatcctc gtccttacaa agtcgccatg ctgatgcaag cgccatatta cttccaggaa
    16021 gcgcagattg aagccgcgat cgccgcgatg gatgttgcac ctgaatatgc cgatatacgg
    16081 caggtggagt catcgacggc ggtgttgtac ttgtttagcg agcgatttat gacctatggc
    16141 aaggcttatg gcttatgcga atggttcgag gtagagcagt ttcagaaccc ctgaaatttc
    16201 agggagatgt catggatagg gtggacagtt gcagccactg cattagggga taatcagatg
    16261 tccttcactc gacgcaaatt tgttctgggg atgggaacag taatcttttt tactggttct
    16321 gcgtcttctc tgttagcgaa cacgaggcaa gaaaaggaag ttcggtatgc catgattcat
    16381 gacgagtcac ggtgcaatgg ctgtaatatt tgcgcccgcg catgccgtaa aactaaccac
    16441 gtccccgctc agggaagccg tttatcgata gcacacattc cagtcaccga taacgacaac
    16501 gaaacgcagt atcacttttt tcgccagtca tgtcagcact gcgaagatgc tccgtgcatc
    16561 gacgtatgcc cgacaggggc gtcgtggcgt gatgagcagg ggatcgtgcg ggtagaaaaa
    16621 tcgcagtgca tcggctgcag ttattgcatt ggcgcttgtc cgtaccaggt tcgctacctt
    16681 aaccctgtca ctaaagtggc agataaatgt gatttctgcg ctgagtcccg actggcaaaa
    16741 ggctttccgc ccatttgcgt cagcgcctgt ccggaacacg cattgatctt tggtcgcgaa
    16801 gatagcccag aaattcaggc gtggttgcag gataataaat actatcaata tcagctacct
    16861 ggtgccggga aaccgcatct gtatcgccgg ttcggtcaac atttgattaa aaaggaaaat
    16921 gtatgaaccc gtcgcaatat gctgaacagt ttcagagcca gttagcgaac tatgtgccac
    16981 agttcactcc cgaattttgg ccggtgtggt tgatcattgc cggagtattg ctggttggga
    17041 tgtggttggt gctggggctg cacgccttgc ttcgtgctcg tggcgtgaag aaatcagcca
    17101 ccgatcatgg tgagaaggtt tatctttaca gcaaagcggt cagattatgg cactggtcga
    17161 atgcgttact ctttgtattg ttgctggcaa gtgggctgat aaatcacttt gcgatggtgg
    17221 gcgcaactgc ggttaaaagt ctggttgcgg tgcatgaagt ttgcggattt ttgttactgg
    17281 catgctggct cggctttgtg ctgatcaatg ccgttgggga taatggtcac cactatcgca
    17341 ttcgtcgtca ggggtggctg gaacgagcgg caaaacaaac gcgattttat ttgtttggca
    17401 ttatgcaggg ggaagaacat cctttcccgg caacaacaca gtctaaattt aatcccttac
    17461 agcaggtcgc ctatgttggt gtcatgtatg gattgctgcc gttgttacta ttgacggggc
    17521 tgctgtgtct ctatccgcaa gccgtgggag atgtgtttcc tggcgtaaga tactggttat
    17581 tgcaggcgca ttttgctctg gcatttataa gcctcttttt tatcttcggt catctttatc
    17641 tttgcaccac ggggcgtacg ccacacgaaa cctttaaaag catggtcgat ggctatcacc
    17701 ggcactaacg aatgatcatt acacgagcag acctgcgaga atggcgtatc ggagcggtga
    17761 tgtatcgctg gttcctgcgc catttcccac gcggcggtag ttatgccgat attcatcatg
    17821 cactgattga ggaaggatat acggactggg cggagagtct ggttgagtat gcgtggaaaa
    17881 aatggctggc ggatgaaaac ttcgcgcatc aggaagtttc ctctatgcaa aaactggcca
    17941 cagaccccgg agagatacca ttttgcagtc agtttgcccg cagcgacgat catgctcgca
    18001 tcggctgttg tgaggataac gcccgaatcg caaccgctgg gtatgccgcg caaattgcca
    18061 gtatgggtta tagcgtgcgg attggaagtg tcggctttaa cagtcacatt ggcagctccg
    18121 gtgaacgcgc acgggttgct gtaaccggta attcttcgcg gataagcagc gctggagata
    18181 gtagtcggat tgcgaatacc ggaatgcgag tacgcgtttg tacattgggt gaacggtgtc
    18241 atgtcgccag taatggtgac ctggtgcaaa tcgccagctt tggcgcaaac gccagaattg
    18301 ccaacagcgg cgacaatgta cacatcattg ccagcggtga aaattcaacg gtagtgagca
    18361 cgggcgtggt cgactcaatt attctcgggc cagggggaag cgccgcactg gcatatcatg
    18421 acggcgaaag agttcgcttc gccgtcgcca ttgaaggtga aaacaatatt cgaaccggag
    18481 tccgctatcg tcttaatgaa cagcatcagt ttgttgagtg ctgatagctt tacctttaat
    18541 caaacgaaag acgccgacga gcacgggatg caggctttac gaccgcccga gccatcgcct
    18601 caccaatttc tgcacatgcc gtaagtccct gaacgaaagg ctggtcgtgc atcaaccagg
    18661 gcaacgcgcc gaacgctacg cgtccacgaa tatcttcggg ttgctgtaac gtataatctt
    18721 cgccaacatc agggatttca tcccctgttt tctgtaattg ctcgcgtagc ccagggaaag
    18781 gaatatcttt cactttaagc ggacgctgtc cgcgggcatc aataaaaacg tcaaacgagt
    18841 agctgttgtt ttccgttttc aggacggtgc gcgactcatt aatttccatt ttgtagtctt
    18901 caccgagggc gagaatatga atgattcccg cttcacgtaa ggcaagtagg cgacgaatag
    18961 actctgaagg gattgccgca taattatcga tgaaaaccct ggcaaggcct ttactgaacc
    19021 gtttatggtc gtgttcattc agatgaggaa caatttcctg tacggcttca tgcaggcgca
    19081 gaatgacata acgccaggga acagtatgtt tttctcgttt attgcgttcg acttcctgga
    19141 gatttttttc tgcccagtca aatgggtcgc gttgtttgcg ctcggcaaac caggcttgag
    19201 caaaggaatc gacattcagg ctctctaagg ctatgcgttg gctccagtct ggatcagcaa
    19261 acttgatttc ctctactatc aatctaaata cccgatccaa aaggccctct tcaccttttt
    19321 gaatctcagc atttaatgcc tgatcggtga cgatgtgtaa gggctcgtag ggaatagggc
    19381 aatagaaatc ggcttcgggt aaaatacccg tgcgcgacat caacgtaata tttagctttt
    19441 cacttgcgtt atcgcggtta aaaacgacgt gttgtttatc atcttcaatg aacgaaccat
    19501 gctgaatagc cactgccatt gccgcatcca gtccgctcaa ggatgttccc ataataccca
    19561 cgttacacgc atcgaccttt gcttccatca ggcctgacca cggactggga aaatacgttc
    19621 tggttgcttc ttcttcatca ggccagacgt gacccgtggc aatcaccgct aaatcaaacg
    19681 tctcgctggg taaatcctga tttgtagcga gcatgacgcc agcatttgta atttgcagat
    19741 cggtaacctg gcatgattca taaacagcca ctgcaaattt ttgctgtcgt gcctggtcta
    19801 ctaatcgtaa aaattgatcg cggaaatatt cgcccagcag aattcgcggt aaaaactgac
    19861 gatcgtgcaa ggtttctttt ttaacgccat aacgctggag atggctggct tcttgctttt
    19921 gtagccattc gagatacgta caattaatcg gcggtatttc aatactggca atatttgcca
    19981 gcatcatttt tgagttttcc tcatcactgt atggcatccc gacaccggcc tcgtcagcct
    20041 gctcgaagat agaaatagaa agtggagttt gttgctgtag aagcgagaat aaggtgtaga
    20101 tccccgtagg cccggcaccc acaatagcaa tttttttcat tgttacttac ctgtacgcat
    20161 tctttcgaat ttagtgttat cagataagtt tggttgagat ttttagaatt tgagaaattt
    20221 cagacaggga tgaaaagggc gctaaccgcc cttgattaaa ttctgtcagg cgagttttgc
    20281 ctgattgatt tgactcagtg gctcattgac atcgaaaact ttggcgacaa cttcctcaac
    20341 gccgagattt ttcagtcggg cagtgtgttt ttccagatag gcaagggcgc ttttttcatc
    20401 agtgaaaagg tagatcccac cggcttcgtg gttcttttca ctttcggtcc ataccttcca
    20461 cagaaaacca ggttcctgat taatcgactc agcaagcggt ttaagctgct cagccattgc
    20521 gtcgccaaat gggccattga aagcaaaatg aagttgtaac aaagttgcca tagcgattct
    20581 ccttctgata acgcatacga agacagtgta aatcttttaa cgtattggat acagagaaga
    20641 aacagaggga aaaaggagaa gaccagatta agaaaatctg gtgcgtccga gtggactcga
    20701 accaccgacc cccaccatgt caaggtggtg ctctaaccaa ctgagctacg gacgcaggat
    20761 ggtgcgttca attggactcg aaccaacgac ccccaccatg tcaaggtggt gctctaacca
    20821 actgagctat gaacgcaacg ttgtaggtga caacggggac gaatattagc ggcagagtgg
    20881 gaaggtggca agaggcaaaa cgtaattttc tgcgctattt cgaccgtttg cagagctttt
    20941 aagcaaattg gctatatttt gttgatttgc aagggcgatt tttattcagg atcgcattta
    21001 catctgatac aacccaataa ttcgtgtatc agagtttaaa cagagttgtt tgccaccatc
    21061 tcaaatctgt cgtggataaa tcctccgtag cctttacact taggtagcaa taaaggcaca
    21121 cagatgcagc aaaggaataa atcatgggat ctgatgcgaa aaacttgatg agcgacggga
    21181 atgtgcaaat tgttaagacc ggcgaggtca ttggcgcgac gcaacttact gaaggcgagt
    21241 tgattgttga agctggcgga agagccgaaa ataccgtggt cacgggggct ggctggttga
    21301 aagtggcaac cggtgggatc gccaaatgca cacagtacgg taacaatggc acgctatcgg
    21361 tcagcgacgg tgccattgcc acagatattg ttcagtccga gggaggcgca attagtctct
    21421 ctacgctcgc tacggttaat ggccgccatc ccgaaggtga attcagcgtt gatcagggtt
    21481 atgcctgcgg tttgttgctg gaaaatggcg gtaacctgcg tgtactggaa ggacatcgcg
    21541 cggaaaaaat cattctcgat caagagggcg gcctgttggt taatgggaca acctcagcgg
    21601 tcgtggtaga tgaaggtggt gaattgttgg tgtatccagg tggggaagcc agcaattgtg
    21661 agattaatca gggcggcgtt tttatgctgg ccgggaaagc cagtgatacg ttgcttgctg
    21721 gtggcaccat gaataatctc ggtggtgaag actctgacac tattgttgag aatggatcca
    21781 tttatcgtct ggggacggat ggccttcagc tctacagttc cggtaagacc caaaacctgt
    21841 ccgtgaatgt gggtggtcgg gctgaagtgc atgccggtac gctggaaaat gcggtaatac
    21901 aaggtggaac agtgatcctg ttgtcaccca ccagcgcgga cgaaaatttt gtcgtagagg
    21961 aagatcgcgc accggttgaa ctgaccggga gtgttgcatt actggacagc gcttcaatga
    22021 ttattggtta tggcgcagat ctgcaacaat caacgattac tgtacagcag ggcggtgtgt
    22081 tgattctcga cggcagtacg gtaaaaggtg acagtgtcac ttttggtgtt ggtaacatca
    22141 atctgaacgg cggaaaactg tggctgatca ctggtgcggc aacgcatgtg caactgaaag
    22201 tgaaacgcct gcgcggagag ggagcgattt gcctgcaaac cagtgcgaaa gaaatctcac
    22261 ctgacttcat caatgtgaaa ggggaagtta ccggggatat acacgttgag ataacagatg
    22321 ccagtcggca aactctgtgc aacgctctga aattacagcc agacgaagac gggattggcg
    22381 caacgctcca gcctgcgtaa aaacacaggc gcaacccgtg aaggttgcgc cttgtcttta
    22441 ttagcgggat gctcgttgca gaatgataac tgacggcaga cgttgcagga accgcatacg
    22501 cagcatcatc ataatggctg ccgacgtcag gccaataata aagcctatcc agaagcctgc
    22561 tggccccata ggttcaacga ccagatcggt cagtgccaga atatagccgc ttggcaagcc
    22621 cagcacccag taagccgtaa aggtgatata gaaaatggaa cgcgtatctt tataaccacg
    22681 caaaatccca ctgccaatca cctggattga gtcagaaatc tgatataccg ccgccagcaa
    22741 catcaaatgc gcagccagcg ttacaacctc gggattgtcg ttgtacaaca gggcgatttg
    22801 ctcccgcagt gaaaccgtga atatggccgt cagggttgcc atacagacac ccaccataag
    22861 cccggtccgc gcagcggttt gcgcatccag cgttgagccc tgacccagac gataacctac
    22921 gcggatagtt accgctgccg ccagcgacat tggaagcaca aacatcagtg aactaaagtt
    22981 cagggcaatc tggtgtcctg cgacatcaac aataccgagc ggagacacta acagagccac
    23041 gacggcaaac agtgtcactt caaagaatag tgccagcgca atcggcaaac cgagttgaat
    23101 cagtcgtttc ataaccgcag gatcgggttt cgcggtgcct ttttcgttac gaatatcgcg
    23161 catggagcgg gcgcgtttaa tgtaagaaac catggcaagg aacatgaccc aatacaccgc
    23221 cgcagtagcc acgccacaac caacgccacc gagctcaggc ataccgaaat gaccataaat
    23281 aaagatatag ttcaccggaa tgttcaccag caggccgata aagcccatta ccatacccgg
    23341 cttggttttt gccagacctt cacattggtt acgggcaacc tggaagaaca gatagcccgg
    23401 cgcgccccac aacaacgcac gcagataacc cacggctttg tccgccagag ccggatcgat
    23461 gttttccatg gagcggataa tgtaacctgc attccacagc acaagcataa tgagaacgga
    23521 aacaaaacct gccagccaga aaccttgtcg cacctgatgc gcaatgcgct cacgtcgacc
    23581 ggaaccattt aattgcgcga taaccggcgt taatgccagc agcagtccgt gaccaaagag
    23641 gatcgccgga agccagatag aagtaccgat agcgaccgcc gccatgtcgg tggcactata
    23701 gccgcccgcc atcacggtat cgacaaaacc catcgcagtt tgggcgattt gcgcgagaat
    23761 caccgggatt gctaatgcta ataacagacg cgcttcactg atatacttct gcacgtgaac
    23821 accttttatt tgtagttata tgagagacta aaaagccgcc tgaatgggca gcaaaaagaa
    23881 gaagcagggg aaattcagtc tattgtagcg aggatttatt aattctccag tgaaaaaata
    23941 gtcattctta ccccttcact ggtaagggcg tttgccacct gctattgtgc tgaacagaat
    24001 gtcttaactg atttcaggag ttgtaagtat gtttacgggg attgtacagg gcaccgcaaa
    24061 actggtgtcg attgacgaga aaccaaattt tcgtacgcat gtggtggagt tacccgacca
    24121 catgctggac ggcctggaaa caggtgcttc cgtggcgcat aacggttgct gcctgaccgt
    24181 gacggaaatt aacggcaacc atgtcagttt tgacctgatg aaagaaacgt tacgcattac
    24241 caatcttggc gatttaaaag tgggggattg ggtaaacgtt gagcgtgcgg cgaaattcag
    24301 tgatgaaatt ggcggacact taatgtcagg tcatattatg accaccgctg aagtggcgaa
    24361 aatattaacc tcggaaaata atcgccagat ctggtttaaa gtccaggata gtcagttgat
    24421 gaaatatatc ctgtacaaag gatttattgg catcgacggt attagcctga ccgtcggcga
    24481 agtcacgcca acgcgttttt gcgtccattt aattccggaa actctggaac gcacgactct
    24541 tgggaagaaa aaactcggcg cacgcgtcaa cattgaaatt gatccacaaa ctcaggcagt
    24601 ggtagatacg gtagaacgtg tgctggcggc acgagaaaat gccatgaatc aaccaggtac
    24661 agaagcctga tctgaaatcg cccccggaga gggggcgata gaatagcctt tagcgagcga
    24721 ctcgaaggcc gttttcaaca ccgcgtgaga acacgacctg ccagagctga atatcacggg
    24781 cgcggaaagc acctgcacag gcattcagat aataggtaaa cattcgttta aagcgttcac
    24841 tgtagttatc cgcaatttct ggccatgcgg cgaggaatcg ttcataccac gccatcaacg
    24901 tagtatcgta atcagcaccg aagttatgcc agtcttccat cacgaagtgg ggttcgctgg
    24961 attgagcaat ctgacgtaca gagggcaggc aaccgttcgg aaaaatatat ttattaatcc
    25021 agggatcaac attcagatcg gtttttttcg aaccgatagt atggagcagg aatatacctt
    25081 ccggtttcaa attacgatcc accaccgcaa aataggtatc gtaatttttc ggtccgacgt
    25141 gctcgaacat ccccacagaa acaatacgat caaactggtc gttcaggtca cgataatctt
    25201 gcagcaaaat ggtgacatcc aggccttcac agcgttcctg agccattttt tgctgttcgg
    25261 cagaaatggt gacgccgacc acgcttacgt catagttaga tgccatgtag tgcgccagtc
    25321 cgccccagcc gcagccaata tccagtacgc gcatccctgg ttttaactgc aatttttcac
    25381 aaatcatttt gagcttcgcc tgctgggcag attccagatt atcggcatct ttccagtaag
    25441 cgcaggaata ttgcatgaag ggatcaagca tgcggctgaa caagtcatta cccaaatcgt
    25501 aatgctcctt gccgactatc caggcacgtt ttttactctg cagattgaag agacgagcgc
    25561 tggcaatacg cagcgtgtct ttgaaatgat gggggagttg gttctcgaga cctgcgcgta
    25621 agactttgct aaaaaacata tccagtcggt cacattccca ccagccatcc atataacttt
    25681 cgcctaaccc caaagagcct tcttgcagaa cgcgtttaaa aaaatcgggg tttttcacac
    25741 gaatatccgc cggggcagaa ccgttaatgg ctataccggc acggctaagt aattcgttgg
    25801 cgatacggta ccagttgtca tccggtacac tgacttcttc tatacacgat gaactcatag
    25861 tttctccatc actggtcggt gatcagaacc ttaaaacagc gtagacgctt tttgggcttt
    25921 gtgagaaatc tcgcagaaaa aaccgcgagt ctataatccg acgtagaaca gagggaaagg
    25981 aggaaccttt gccgaaatga ccatccatag tgagtcggga acgatcctgt ccccgtaaaa
    26041 acataatatt tatcgtagct ttaacaactc gaaattagta taggccgcta atttcaatca
    26101 tacaacaaaa ttttgttagc agcctaagta taagtatatc gatatagatc agtgtgattc
    26161 gctatgagcg acttcggcat tgccatgatt ctggcagcca acttcttcac aacgttgcat
    26221 catgtaaccc agagcgacca gcactactgt tgataacatc acgctggtgg tggtgagcaa
    26281 tggcgtgctg atactgatca gccaggaaac taccagactt gcgaggaagc acagacccag
    26341 ttgtagagtg ttctgcaatg ctgcggcgcg accagttgcg tgtgggaagg gacgcagcgc
    26401 ctgggcgaca acaatcgggt agatcgcgcc attggcaatc gccatcacac agaatgggat
    26461 caggatttcg accagagaca catggctaat gaagcctgca gcccaggtcg caatgacgct
    26521 gactgcaaac agcaccagca accacggtaa taactgcttg ccttgccatt tctgcagcgc
    26581 ggcgcgacag ccataaccac caatcagaaa cgcgatagtt tgcgggacat aacttaaacc
    26641 aataactgcc gggctgtagc ccatttcact aaggatgaac ggtgaaccgg tcagccatgc
    26701 aaaaaaactg gctgaacagg ccgcgtatat cagcacgttg ccgcgatagg ttttagaacg
    26761 tagcaggtcg gtaaaggtca gaccatcctg actattgtta cgggccttcg tcgtgggttt
    26821 gagccagaaa ataggcagaa tcagcaccac ggtaatggca aacagggtgg cgaaaatcgc
    26881 ctgccaggaa aaatggacca gcagccagct tcctaacaga ggagccaggg ccggagatag
    26941 acccaccagc ggcatgatgg tcgcaaaaat tcggttaact ttctgtgaag gataataatc
    27001 cgtcactaac gcttgccaga taaccgccgc ggcgcagaca cccacagcct gtacaaaacg
    27061 caataccagc agcgtagcgg cgttttctac ccacagcatc cccagactac ctaacgcaaa
    27121 aattgtcagg ccgattaata acaccggttt acgaccataa cggtcggaga gtggccccca
    27181 cagaagctgg gctgcggcaa aaccggcaag gaacagacta aggctggcac tgacagcaga
    27241 cgcaggcgtt tgcaggtcgg cctgtatggc ggcgaaagca ggcagat
//
LOCUS       DAEVZA010000059        27177 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEVZA010000059 DAEVZA010000000
VERSION     DAEVZA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.059"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..144
                     /locus_tag="LAF92_002531"
     CDS             <1..144
                     /locus_tag="LAF92_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="response regulator in two-component regulatory
                     system with EvgS; phosphorylated EvgA activates ompC,
                     emrKY, gadABC, hdeAB and others genes; involved in acid
                     resistance, osmotic adaption, and drug resistance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBI9791520.1"
                     /translation="GKDNNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRN
                     KIG"
     gene            149..3742
                     /gene="evgS"
                     /locus_tag="LAF92_002532"
     CDS             149..3742
                     /gene="evgS"
                     /locus_tag="LAF92_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBI9791521.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(3798..4943)
                     /gene="yfdE"
                     /locus_tag="LAF92_002533"
     CDS             complement(3798..4943)
                     /gene="yfdE"
                     /locus_tag="LAF92_002533"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBI9791522.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWEMLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5017..5961)
                     /gene="yfdV"
                     /locus_tag="LAF92_002534"
     CDS             complement(5017..5961)
                     /gene="yfdV"
                     /locus_tag="LAF92_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBI9791523.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6031..7725)
                     /gene="oxc"
                     /locus_tag="LAF92_002535"
     CDS             complement(6031..7725)
                     /gene="oxc"
                     /locus_tag="LAF92_002535"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBI9791524.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(7779..9029)
                     /gene="frc"
                     /locus_tag="LAF92_002536"
     CDS             complement(7779..9029)
                     /gene="frc"
                     /locus_tag="LAF92_002536"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBI9791525.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(9542..10174)
                     /locus_tag="LAF92_002537"
     CDS             complement(9542..10174)
                     /locus_tag="LAF92_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBI9791526.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            10508..10783
                     /gene="ypdI"
                     /locus_tag="LAF92_002538"
     CDS             10508..10783
                     /gene="ypdI"
                     /locus_tag="LAF92_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HBI9791527.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(10860..11102)
                     /locus_tag="LAF92_002539"
     CDS             complement(10860..11102)
                     /locus_tag="LAF92_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBI9791528.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            11455..12375
                     /gene="lpxP"
                     /locus_tag="LAF92_002540"
     CDS             11455..12375
                     /gene="lpxP"
                     /locus_tag="LAF92_002540"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBI9791529.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVKLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            12731..12802
                     /gene="ypdK"
                     /locus_tag="LAF92_002541"
     CDS             12731..12802
                     /gene="ypdK"
                     /locus_tag="LAF92_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBI9791530.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(12867..14105)
                     /gene="alaC"
                     /locus_tag="LAF92_002542"
     CDS             complement(12867..14105)
                     /gene="alaC"
                     /locus_tag="LAF92_002542"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBI9791531.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            14482..16179
                     /gene="ypdA"
                     /locus_tag="LAF92_002543"
     CDS             14482..16179
                     /gene="ypdA"
                     /locus_tag="LAF92_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBI9791532.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            16194..16928
                     /gene="ypdB"
                     /locus_tag="LAF92_002544"
     CDS             16194..16928
                     /gene="ypdB"
                     /locus_tag="LAF92_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBI9791533.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            16941..17798
                     /locus_tag="LAF92_002545"
     CDS             16941..17798
                     /locus_tag="LAF92_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9791534.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(17801..20296)
                     /gene="ptsP"
                     /locus_tag="LAF92_002546"
     CDS             complement(17801..20296)
                     /gene="ptsP"
                     /locus_tag="LAF92_002546"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI9791535.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(20321..21358)
                     /gene="ypdE"
                     /locus_tag="LAF92_002547"
     CDS             complement(20321..21358)
                     /gene="ypdE"
                     /locus_tag="LAF92_002547"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI9791536.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYSAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(21358..22443)
                     /gene="ypdF"
                     /locus_tag="LAF92_002548"
     CDS             complement(21358..22443)
                     /gene="ypdF"
                     /locus_tag="LAF92_002548"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI9791537.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(22458..23705)
                     /locus_tag="LAF92_002549"
     CDS             complement(22458..23705)
                     /locus_tag="LAF92_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBI9791538.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(23727..24053)
                     /locus_tag="LAF92_002550"
     CDS             complement(23727..24053)
                     /locus_tag="LAF92_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBI9791539.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(24272..25237)
                     /gene="glk"
                     /locus_tag="LAF92_002551"
     CDS             complement(24272..25237)
                     /gene="glk"
                     /locus_tag="LAF92_002551"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBI9791540.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHNNPGLLGSGAHLRQTLGHIL"
     gene            25441..26697
                     /locus_tag="LAF92_002552"
     CDS             25441..26697
                     /locus_tag="LAF92_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBI9791541.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHSMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            26812..27138
                     /locus_tag="LAF92_002553"
     CDS             26812..27138
                     /locus_tag="LAF92_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBI9791542.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
BASE COUNT         7096 a         6822 c         6343 g         6916 t
ORIGIN      
        1 ggcaaggata ataatgacat tgctgaaaaa atgttcatca gcaacaaaac tgtcagcact
       61 tataaaagtc gcctgatgga aaaattagaa tgtaaatcac tgatggatct ttacacattc
      121 gcgcaacgta acaaaatcgg ctaatcacat gaagttttta ccctattttt ttcttctctg
      181 ttgtggtctt tggtcgacca taagtttcgc agacgaagat tacatcgaat atcgtggcat
      241 cagtagtaac aaccgtgtca cacttgatcc agtacgtctg agcaacaagg aattacgttg
      301 gttagcgagc aaaaaaaatc ttgtgattgc agtacacaag tcccaaacgg ctaccttgtt
      361 gcataccgat tcgcagcaac aggttcgtgg tattaatgct gattatttaa atcttttaaa
      421 aagagcgtta aatatcaaat taacactccg ggaatacgcc gatcatcaaa aagcaatgga
      481 cgcgcttgca gaaggtgaag tcgatatagt gttatcacat ttagttactt cgccgcctct
      541 taatagtgac attgctgcaa ccaaaccatt gataattacc tttccggcgc tggtaaccac
      601 ccttcacgac tcaatgcgac cgcttacctc accaaaacca gtaaatattg ctcgggtagc
      661 aaattacccc ccagacgagg taattcatca atcgtttcca aaagcaacaa ttatctcttt
      721 tacaaattta tatcaggcat tagcatccgt atcagccgga cagaatgatt actttattgg
      781 tagtaacatc attaccagca gtatgatttc ccgctatttc actcactcct taaatgtagt
      841 gaaatattat aactcgccgc gtcaatataa ttttttcttg accagaaaag aatccgtcat
      901 tcttaatgaa gtactcaata gatttgttga tgctttaaca aatgaagttc gctatgaagt
      961 ttcacaaaat tggcttgata caggaaacct ggcctttctg aacaaaccat tagaactcac
     1021 tgaacatgaa aaacagtgga ttaagcaaca tcccgattta aaggtgctgg aaaatcctta
     1081 ctctcctccc tattctatga cggatgaaaa tggctcggtt cggggcgtta tgggggatat
     1141 tcttaatatt attaccttgc aaacaggttt aaatttttct ccgatcaccg tttcacacaa
     1201 tatccatgct ggaacacagc ttagccccgg aggatgggat ataatacctg gcgctattta
     1261 tagtgaagat cgagaaaata atgttttatt tgctgaagcc ttcataacaa cgccttacgt
     1321 ttttgtcatg caaaaagcgc ctgacagtga acaaacatta aaaaaaggaa tgaaagttgc
     1381 cattccatat tattatgagc tgcattcgca attaaaagag atgtatccgg aggttgaatg
     1441 gatacaggtc gataatgcca gcgctgcatt tcacaaggtt aaggaaggtg aacttgatgc
     1501 tctggtcgcg acacagttaa attcgcgtta catgatcgat cattactatc ctaatgaact
     1561 ttatcatttt cttattcctg gcgttccgaa tgcatcgctt tcgttcgctt ttcctcgcgg
     1621 agaaccggaa cttaaggata ttattaataa agcactgaat gcaattcccc caagcgaagt
     1681 tctgcgcctg acggaaaaat ggattaaaat gcccaatgtg accattgaca catgggacct
     1741 atatagcgag caattttata ttgttacgac attatccgtt ttattagttg gcagtagcct
     1801 tttatgggga ttctacctgt tacgctcagt tcgtcgtcgt aaagtcattc agggtgattt
     1861 agaaaaccaa atatcattcc gaaaagcact ctcggattcc ttaccgaatc caacttatgt
     1921 tgtaaactgg caaggtaatg tcattagtca taatagtgct tttgaacatt atttcactgc
     1981 ggattactac aaaaatgcaa tgttaccatt agaaaacagt gactcaccct ttaaagatgt
     2041 tttttctaat gcgcatgaag tcacagcaga aacgaaagaa aatcgaacaa tatacacaca
     2101 ggtatttgaa attgataatg gcatcgagaa aagatgcatt aatcactggc atacattatg
     2161 caatcttcct gcaagtgaca atgcagtata tatttgtggt tggcaagata ttactgaaac
     2221 gcgtgatcta attaatgcac tcgaggtaga aaaaaataaa gcgataaagg ctaccgtagc
     2281 aaaaagtcag tttctggcaa cgatgagtca cgaaataaga acaccaataa gctctattat
     2341 gggcttcctg gaacttctgt cgggttctgg tcttagcaag gagcaacggg tggaggcgat
     2401 ttcacttgcc tacgccaccg gacaatcact cctcggctta attggtgaaa tccttgatgt
     2461 cgacaaaatt gaatcgggta actatcaact tcaaccacaa tgggtcgata tccctacttt
     2521 agtccagaac acttgtcact ctttcggtgc gattgctgca agcaaatcca tcgcattaag
     2581 ttgcagcagt acatttcctg atcattacct ggttaagatc gatcctcagg cgtttaagca
     2641 ggtcttatca aatttactga gcaatgctct caaatttacc accgaggggg cagtaaaaat
     2701 tacgacctcc ctggttcaca ttgatgacaa ccacgctgtt atcaaaatga cgattatgga
     2761 ttctggaagt ggattatcgc aggaagaaca acaacaactc tttaaacgct acagccaaac
     2821 aagtgcaggt cgccagcaaa caggatctgg cttaggctta atgatctgca aagaattaat
     2881 taaaaatatg cagggcgatt tgtcattaga aagtcatcca ggcataggaa caacatttac
     2941 gatcacaatc ccggtagaaa ttagccagca agtggcgact gtcgaggcaa aagcagaaca
     3001 ccccatcaca ctacctgaaa agttgagcat attaatcgcg gatgatcatc cgaccaacag
     3061 gctattactc aaacgccagc taaatctatt aggatatgat gttgatgaag ccactgatgg
     3121 tgtgcaagcg ctacacaaag tcagtatgca acattatgat ctgcttatta ctgacgttaa
     3181 tatgccgaat atggatggtt ttgagttgac tcgcaaactc cgtgagcaaa attcttcctt
     3241 acccatctgg gggcttacag ccaacgcaca ggctaacgaa cgtgaaaaag ggttaagttg
     3301 cggcatgaac ttatgtttgt tcaaaccgtt gaccctggat gtactgaaaa cacatttaag
     3361 tcagttacac caggttgcgc atattgcacc tcagtatcgc caccttgata tcgaagccct
     3421 gaaaaataat acggcgaacg atctacaact gatgcaggag attctcatga ctttccagca
     3481 tgaaacgcat aaagatctac ccgctgcgtt tcaagcacta gaagctggcg ataacagaac
     3541 tttccatcag tgtattcatc gcatccacgg tgcggctaac atcctgaatt tgcaaaagtt
     3601 gattaatatt agccatcagt tagaaataac acctgtttca gatgacagta agcctgaaat
     3661 tcttcagttg ctgaactctg taaaagaaca cattgcagag ctggaccagg agattgctgt
     3721 tttctgtcag aaaaatgact aaatagcggc tcccacaatg ttcaaatgtg ggagctattt
     3781 accagcacat cttacattta tgatgagaac tcctggcgaa tttgttcccc atgctggtcg
     3841 agcgttgccg ctcccggcat aacatgcggg tccgcgcagc cgctgatttt tatcggatta
     3901 cccggcatca ttattccccc ggcttcaatc aacatatttc tcgcctgagt ttgtggcaat
     3961 ttaatggcct cagccacact taataacggc gcgacgggta caccaacttc atgtattctg
     4021 gctaaccaaa cttcagctgc ctgcgttttt aacgtccgct caatatattg tttaagaata
     4081 gcctggtttt gtacgcgtaa aatattgctg ctaaatcggg gatcattaac cagttccgta
     4141 agctccagtg cctggcataa cgcagaaaaa agcttgtcat taccacaaca aatcgtaatc
     4201 ggcttatcct gagtattgaa aacatcaaaa ggtgccatgt agggatggcg atttcccaga
     4261 cgttgtggtg acttccctgt cgcgatatat gccatcagac catgctccag aaaactcagc
     4321 gtggcatcaa acatcgctat atcgacatgc gcccctctct ggctcttttc gcggccataa
     4381 agtgcactca ctattccgct gaataaatag acaccgccgc atagatccgc aagagatgta
     4441 ccaacgcgca ctggcggagc atcaggatat cctgtttcca tcattatccc gctcattgcc
     4501 tgaatgatgg tatcgtaggc aggagcatct tttagcggac cggtatgtcc gaaacctgac
     4561 gatgaagcat atatgaggcg cgggttgatt tcttgaagca tttcccatga aaaccccagt
     4621 ttttccattg tacctgggcg aaaattctca gctaatacat cagcttgttt gagcatattt
     4681 ataaatatac ttttatcgtg atcattcttt aaatcaagaa ccacactctc tttgccatga
     4741 ttaataaaac tgtaatagag tgactgtcca tccacatagg gaccaaatgt gcgggtatca
     4801 tcaccatgac ctggtggctc aactttaatt acccttgcgc ccatattaca aagaagttga
     4861 gttccgaaag gtccattaag gacatgtgtc atatcgataa ctaataagcc ttcaaacggc
     4921 cctttgcttt cattatttgt catttgctta tgccttgaaa aaagccgaag gtgaaaagac
     4981 acttcggcat ttatgtaaaa tcaataacat gattaattat gaaaccagtc gactgacata
     5041 aatccacaag ggagccgtga cgacaaagcc cagtacgctc acagccaatg acgctgtacc
     5101 agtacgggtg tagacattaa accggctggc aataatgatt ccggagaatg ccggcggtaa
     5161 tgcgcctgcc agtaccatca tctgcagatg ttcgctgttc aaatgacatg ccataccaac
     5221 gagaagcagt gccagtggca tcagaatcag cttcaggaag gtgttataag caatttcggc
     5281 actgaactcg aatttatgtg cagccagagt caaccccgca gcgaataccg ctacccctga
     5341 gttagccttc gcaatcagat taaaggttgg gtcccatgct gccggaattt ttaccccaac
     5401 caacaccagg atcgttgcca gaacaggtgc ccatactact ggctcctttg ccgcagaaat
     5461 taatgcgctc agattactat tcttcttacc atccgctcct gaggaaggat tcagcaaata
     5521 cagaccaata ggaatagtaa ttgcgttaac aataatagaa ataattgcta ccactaaacc
     5581 tgttgatacg gaatcaccat aaataggatc gagaactgca aaccccagga atccaatggt
     5641 aggtgaaccc gcaattaatg cacatacagc tgcttctgca tgggtacgtt taaaaaattt
     5701 gtagcaacca aaccaggaga aaaagaaaca tccgacaata accacaagtg atacaagggt
     5761 cagacgagtg tccgcaaaaa tcatttccct gtttgcccgg gtaatagaaa caaatagagc
     5821 cgcaggaagc gcgtagttta ataccagttt attaaatgcg cgggcttgat cttctgaaaa
     5881 agtttctcgt ctgccgctaa aataacccaa cagcatgatt acgataatag gcaataaatc
     5941 gccaataaaa aatgttagca taatagtatt tccgataagg gtcccctcac catcgcataa
     6001 tgagttggca agaggagacg atgtcagtat ttaattacca gcgacttgtt ttgggttaag
     6061 tttggtaata tggccacttt cagttcctgc tgcagggtcg atgaccacat taataatggt
     6121 cggtttgcgc gactgaatac cggtggttaa agcatgacga agttcatctg tcgtggtgac
     6181 gttatagcca acgccacgaa acgcatccat taatttgtca taccttgcat ggtgcaacag
     6241 atccgttggt gatggtgcac cagcgccact gagatcaaca ccgtctcctc tgtagatgcc
     6301 gccattatta aaaataacga tcgtcaccgg caggttatat cgacaaatcg tttcaatttc
     6361 catcccactg aaaccaaaag cactatcacc ttcaatggcg acaaccggag aaccagaagt
     6421 cacgctagca ccgatggcat agcccatacc gatgcccatg acaccccagg taccacaatc
     6481 cagacgacga cgtggtttat acatatcaat aatatttcgt gcattatcca gggtatttgc
     6541 accttcatta actaaataaa tatcctggtt ctcgcgcaat acgtcgcgca cagcacttaa
     6601 tgcattaaag taatttaatg gttgagtatc tgtacttaat ttttcatgca ttttttgtgc
     6661 attttgctgc ttgtggatat ttaaaatatc gcgccatacc agtggagtcg taaatgtgtt
     6721 ttgtttcagt tctgccagca taccttgcat actggatgca atatcaccaa cgactggcac
     6781 agcaatgggg cggttgctgt caatttcctg cggttcaata tccagttgaa taaactgtgt
     6841 atctgccgcc catccttttt taccgtgtgc caataaccaa ttcagtcgtg caccaacaag
     6901 catgacaacg tcagcatttg ccagggcaaa cgaacgcgca gctgccgcag aaagtggatg
     6961 cgtatcttca aggatccctt tcgccataga cattggcagg aatggaatct gagtactttc
     7021 aataaattca cgaagctgtt catcagcttg tgaatacgcc gcgcctttgc caaggataat
     7081 taatggccgt tcagcttttg ctaaaagcga aattgcgcta gtgactgact tcgggcatgg
     7141 caataatgct ggcgacggat tttcaacttt aacaatcgtg gttaacgctt cgtctttttc
     7201 catcgtcgcg gccaggacat ttgctggcaa atcaagataa actccgccag ggcgacccga
     7261 tacagagacc cggatagcgc gtgccaatgc aatgccaaga tcctgcggct gattaacgcg
     7321 aaatgctgct ttggcatacg gttttgccgc attcatttgg tccagctctt cataatcacc
     7381 ttgctgtagg tcgacgatcg cgcggtcgct ggagccgcta atcatgatca tcggaaaacc
     7441 atttaccgtt gcgttggcca atgcggtcaa accattgagg aatcctggcg cagaaactgt
     7501 caggcagatc cccggttttt gggtaagaaa accgcttgcc gcagcggcat agcctgccga
     7561 ctgctcatga cgaaaaccaa tataacgaat gccttccgcc tgggcatggc gtgccatatc
     7621 cgtcacagga atacctacaa caccataaat agtgtcaata ttattctgtt ttaatgcttc
     7681 aacgatgata tgcataccat ctgtcatttg aagttgatct gacatgatta ataacctcga
     7741 aatttttata aattgggagc cagaagcccc cgttaatatc agatggcgtg gttttgcttc
     7801 attgcagcaa tttcatcgtc gctataaccc agctcctgca atacagcagc ggtatgttca
     7861 cctaatagcg gcgcagcttt aatatccggc gtaaaggcag agaatttcat tggacaacca
     7921 acggtcagat attttccacg caacggttgt tccacttcaa caacactgcc actttggcgc
     7981 aaagagggat caagtgaaat ttctttcata cttaaaaccg gtgcacaagg aatatcaaac
     8041 tgagtcaaat aggccaccgc ttcatgttta tcaatagtga cagtgtattt ttcgatttca
     8101 gcaaaaatat cgaaaatatg tggctgtcgt gcatgggctg tactgtatgc cggatcggta
     8161 atccattctg gtttgccgat ggctttacag gtgttttccc agttttgctc ctgaatagtg
     8221 aaataaatat aggcgttagg atcggtttcc cagcctttac atttcaggat ccagccaggc
     8281 tgaccgccac cacctgcatt accaccgcgg ggaactgcat caccaaatgt accattcgga
     8341 tactgcgggt attcttccag ataacccaat ttatcgagac gctgctggtc acgtaatttc
     8401 acgcggcaaa ggttcaatac ggcatcctgc attgacatgg tgactcgttg cccacgcccc
     8461 gttttttcgc gatgcagcaa agcagcaagt aaaccgatca gcaaatgcat tccggtgttg
     8521 ctgtcaccca acgctgcagc gcttaccagc ggcggaccat cccaaaaacc cgtagtggat
     8581 gccgcgccac ccgctgcctg agcaacgttt tcataggctt ttacattcac ataaggcgaa
     8641 cactcatcaa accctttgat cgaaccaaaa atcagacgtg gattgatttc ttgaatatgc
     8701 tcccaggtga agcccatgtg atcaatggcc cctggatgaa agttctcgac taagatatca
     8761 gcttcgcgga tcagcttttc cattacctct ttgccttccg ctgttttggt atttaactca
     8821 atagaacgtt tgttactgtt aagcatggtg aagtaaagcg catcgatatc aggaatatct
     8881 cgcagctggt gacgcgttac gtcaccaacg ccgggacgtt caattttaat aacgtcagcg
     8941 ccaaaccagg ccagcatttg agtacaagat gggccagatt gcacaccggt gaaatcgaga
     9001 actttaattc cttgaagtgg agttgacata gcaataccta ttagttaatt aaatatgtgt
     9061 taatttagcg tttatttaag gcgtaatatc ttctataaat aacccgcaaa atatcgtcgt
     9121 agctgcactc gatattttta aaatcctgta tcaagtgctg aaaatcattt catcgccact
     9181 tccaattcct ttctgcatgg taagtagtga ggacgaaaag aagaatatga taaatattga
     9241 ttcgtggtaa tcagagagag tcgcgacatt tgtgatgtta ttcttacaac tgtaagaagc
     9301 gccggtgatt tacaagtaag ggtatgatta cgctttttaa agcgtaccat aaaacataac
     9361 ataataaatg atttgtgatg acacgggatt attgggattt actttgagga agataaaatg
     9421 cgtgtttata gaaccgcgag cgccaaataa ttttatgacc acaacctgtc gtattgtggt
     9481 cataaaaaat tgtcggctgg agggagttaa atcctttacc tcaatatact gcctgtcagc
     9541 attagttcac aaacagtgct tcgctgtcga cgatgattcc atcttccgca cctttaagcg
     9601 ccaggtttgc ctcgtagtat tgctttttat caagcagttt ctgcgcatca gcaagtgcat
     9661 tacgagtctg tttaagcggc atcagatatt ggttttccat tacgccaaca ccggccaggc
     9721 gaagttcttc cattgcgccc tttttatccc ctttcgccat tttttcattg gcaattttta
     9781 tcgcggcttc tttttcggga gttgctacat aactctcaga aattccgaca gatgcattaa
     9841 tgacaatata ttggtcatca ttgagattgg tctttttacc tggcttagcg aactttgccc
     9901 attcggtact gtcatctgac agcaaagcgg aagcttcatt ggtcagttct tttgcctttt
     9961 ctggatcgcc atgaaacaga gccaggcgcg ctacctgcac gtcacgcatc gcatataaac
    10021 cttgttcaga gatacgctct gctgctgccg ttttctgggt ttgctgaact tgctgagttt
    10081 gctgttgtgc cgctaccggt gcattatcag cagcccatac agttgaagat gccagaattg
    10141 ctgtgaccat cgtagccata attaaacgtt tcatacttac ctcattcttt aacagtttta
    10201 gaaaacgccg ccatcatatt gggtgagcat gaaatgagaa atcagagagc aacggtcatt
    10261 ttgaaagggt tatcttacag ttgtagttat tatcgcaaga agtattttag tcttacaatt
    10321 gtagttatta tcgcaagaag tattttagtc ttacaattgt aggaatctgc ttcctgtcgc
    10381 agagaactat tcttatgtac cacaaagaga tgtcatttat tatatcgatg taaatattga
    10441 ttaattatat gtaaatacag ccataacatc gtaggttctc agtgaaccat tttcaggata
    10501 actttctatg aaagtaaact taatactttt cagcttattt ttattggtct ctattatggc
    10561 atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct ataaagagac
    10621 tgaaaaaaca tcagcgatga caaccacaca ctctacaaaa cttcagccat cacaggcgat
    10681 tttgtttaag atgagagaag atgcgccacc attaaacctc acagaagaaa tcacgcctac
    10741 ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt cacatggcag
    10801 gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat cctgacccac
    10861 tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg ggcaagaaac
    10921 ataaatatcc ccgtcactag ttcattaccg ccagttaacc acatggtgaa gtaataaatg
    10981 agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac gttcagtacc
    11041 tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca taaataaatc
    11101 atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta atagtaagac
    11161 aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcatgt taaatgcaag
    11221 actgttgtgt acggaaaaat atttactttg cacgattaat aatcagtagc tgaaagcagt
    11281 cagcgagtga taaagtacag ataccgcatt catgctcctc gtggttatgt cctgactagt
    11341 ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt gagtgtaccg
    11401 ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt aactatgttt
    11461 ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac atggtttggg
    11521 cttggtgtac tctggctttg ggtaaagctt ccttatcctg ttctctgctt tctcggcacg
    11581 cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc ccgtaaaaac
    11641 ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat tgccgaaaac
    11701 tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg gcccgacagt
    11761 cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc acaaatgcaa
    11821 aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg cggccgcgtg
    11881 atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca gctgatggaa
    11941 tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag aaataatctg
    12001 cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc cgatcaggat
    12061 tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt cgccacaacc
    12121 aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt aacgatggta
    12181 agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga aggctacccg
    12241 acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga gatcatgcgc
    12301 gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt gggagaatcg
    12361 tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc cataataacc
    12421 tcatggtttt tatggctttt ttcttaaggt tttttccttc gctgaatatc taattattac
    12481 cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa gtaaataaga
    12541 aaatgtctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg agcagtttag
    12601 ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc gctttgcccg
    12661 gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta attacgcgga
    12721 gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg tttgggccgg
    12781 tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag ccatcaggct
    12841 cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg tttgctgctg
    12901 gcgggtagta aaccgtcggc acggaacatc gctttaatcc cacgaatcgc ctgacgaata
    12961 cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc gccaaagcca
    13021 atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa ttccagcgat
    13081 cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc cttcggcatt
    13141 tcaaccatcc agcccgcttc atgcagccct ttaaccaata catcacggcg gcgtttgtac
    13201 tgttcggcaa tgtcgcgcac gcactgttga tcgccctcca gcgccgcaat cgccgccacc
    13261 tgcaacggcg taaaggtgcc gtaatcgtga tagcttttaa tacgagccag ggcgctgacc
    13321 agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta gcttttcgac
    13381 agcgtaaaga actcgactgc cacatcgcgt gcacccggta cctgcatgat tgacggcgct
    13441 ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag cacatcgtag
    13501 cgtttcgcca gcgccactac tttttcgaag aactccagtt ccacgcattg cgcggttggg
    13561 tttgacggga agccgaggat catcatcttc ggtttcggat aactttcacg aatggcgcgc
    13621 tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac ctgcgccccg
    13681 gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag caccgtgtca
    13741 ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc aatagtgacg
    13801 atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca gcgggaaatg
    13861 gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc cgggcgctgg
    13921 gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg gttacccatg
    13981 ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc agcggtgatg
    14041 ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg gcgagtgtca
    14101 gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc tgccacaatg
    14161 gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta gaacattttt
    14221 atcaatggct ttaaatgagg aactccactt caaaaacagg aaatttttct acgctaacaa
    14281 gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag ccagaagtgc
    14341 attatcaaga gatacattgc taatacacca tttatctttc aatccccttt taaatttatg
    14401 gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc atagaggttt
    14461 aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc ggtcttcgat
    14521 cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct gtttcgcgaa
    14581 ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc cattttctcg
    14641 ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct ggtgaacgta
    14701 cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg catcattacc
    14761 ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac ggcgatcccc
    14821 tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct gaaaatcccc
    14881 aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac gttgaccatg
    14941 attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt ctctaaaatc
    15001 ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct ggtgcaaagc
    15061 gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct ggatatcgcc
    15121 aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa ggtctgcgaa
    15181 attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga tcatgtgctg
    15241 gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat tagcccgact
    15301 acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga agcccaccgc
    15361 acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt cgtgaccgga
    15421 acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca ggaaatggcg
    15481 gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga gcagctacgt
    15541 gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc ccattttctg
    15601 tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac cgctcgccag
    15661 ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga cgatgagcaa
    15721 atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga gcaggcccgc
    15781 tttggtgaca agctgacggt tatctatgat attgatgaag aggtgaattg ctgcattccc
    15841 agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca gccttgcaaa
    15901 ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg cattgcggtg
    15961 cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc gaatgaaatg
    16021 ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt gtatggcgag
    16081 gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat tcctaaccaa
    16141 cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat attgtgaaag
    16201 tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta attaaagagc
    16261 acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg aagtttttgc
    16321 agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg gatggcgtgt
    16381 tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc atcaccgcgt
    16441 ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt ctcaaaccgt
    16501 atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg caacaacagc
    16561 agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa tgacaccatt aatctggtga
    16621 aagatgagcg aataatcgtc acgccaatta acgatatcta ttacgccgaa gcgcacgaga
    16681 aaatgacctt tgtctatacg cggcgtgaat cctacgtaat gccgatgaac attaccgaat
    16741 tttgcagcaa actgccgccg tcgcattttt tccgctgcca tcgctcattt tgtgtcaatc
    16801 tgaacaaaat acgcgaaatc gaaccgtggt ttaataacac ctacattctg cgactgaaag
    16861 atctggattt tgaagtgccg gtcagtcgca gcaaagtgaa agaatttcgc cagttaatgc
    16921 atctttaatc gggaactttc atgaaagcac caggcttgcc cgccgatcaa caatttttcg
    16981 ccgatctgtt cagcggcctg gtgcttaacc cgcaactact ggggcgcgtc tggtttgcca
    17041 gccagcctgc ctcgttaccg gtgggcagtt tatgtattga ttttccccgt ctggatatcg
    17101 tgctgcgcgg cgaatacggc aacctgctgg aagcaaagca gcagcgaatg gtggaaggag
    17161 aaatgctgtt tattccggca cgcgcggcta atttaccgat caacaacaaa ccggtgatgc
    17221 tgttaagcct ggtgttcgca ccgacctggc ttgggttatc gttttacgat agccgcacca
    17281 cgtcgttgtt gcatcctgct cgccagatcc agcttcccag cctgcaacgc ggtgaaggtg
    17341 aagcgatgct taccgccctc acccatctta gccgttcgcc gctggagcag aatatcattc
    17401 agccactggt gttaagtttg ctgcatcttt gccgcaacgt ggtgaatatg ccgccgggta
    17461 attcgcaacc gcgcggcgat tttctctatc acagcatttg taactgggtt caggataatt
    17521 atgcccagcc gctcacccgc gagagcgtgg cgcagttttt taatatcacg cccaatcatc
    17581 tgtcaaaact gtttgctcag catggaacga tgcgttttat cgagtatgtg cgttgggtgc
    17641 gaatggcgaa ggcgaggatg attttgcaga aatatcatct gtcaattcat gaagtggcac
    17701 agcgttgcgg ttttccggat agcgactatt tttgtcgcgt tttccggcgt cagtttggtc
    17761 tgacgccggg agagtacagc gcccgttttc agggttaacg tcagaaggtt aattctgttt
    17821 ccagcagcgt caggatactt tgtgcatctt gcgcggcaaa caacgactgg cggaagtttt
    17881 tattcaccag tttacgcgcc agctgcgaga agactttcac atgattcatc ccttcgttag
    17941 cacccagcgt cagcatgatc accagttcga cttcgcccat ttctgactgc cagtcaatcg
    18001 gtttcgccag ccgggcaatg ctgatactgg aatgacggat ccactgagat ttggtgtgcg
    18061 ggatcgctac gccaaaacca acgccggtag tgacaatctc ttcccgctgc cagacatctt
    18121 cttccagctc aaacggatgt tcagtacgcc cgttaacgcc gaggttgccg cacaggaact
    18181 ggatcgcctg ctctttattg ctaaaatcct gatcaacaaa gatattctcc agcgccagca
    18241 gtgggcgaac gtcttcttcc ggcgtaaagg cggtgagtaa cgcttcaatt tcctgcgcac
    18301 tgcggcattc acatgcctga cgcgccagtt cccgacacgc ctcgctatcc agttgacgaa
    18361 gctggctttt caccgccgga atacgcgggc tactcatact cagctcatcc aggcccagcc
    18421 caagcagtag cggcagataa cggctttcac cgcccagttc accgcaaatg cctacccatt
    18481 tgccccgctg atgcgcagtg gtaacaatct gctgcaacat gcgcaggaac gatggcgtaa
    18541 tcgggttata cagcggcgat acgcgcgggt tattacgatc gaccgcatac agatactggg
    18601 tcatatcgtt agaaccgata ctgaagaaat cgacctcatc gcagaagtgg tcgatgatgt
    18661 aacacaccga cggcacttcc accatgatac ccagagtaat cgtctctgca tgacgcaggc
    18721 catcgcgctt aagctcaacg atcgcttttt ggatctcgcc cttcacccat aagatctgat
    18781 cgaggctgtg aaccatcggg atcatcaact gggcgttgcc aaaactggca gcgcgcaaaa
    18841 tcgcccgtag ttgagtgcgg aacaggccag caaattccgg gtaaatacgt accgcgcgat
    18901 agccgaggaa cgggttctct tcctggggaa tattcagata aggaatgctt ttatcgcctc
    18961 cgatgtccat tgtgcggaag ataatcggct tgtcgcccgc tgccagtagc acctgctggt
    19021 aggcttcaaa ttgctcctgc tcgtccggcg cgctgtcacg atccatatac agcatttcgg
    19081 tacggaacag cccgacgcct tccgcgccgt tggcaaacgc gcctggtgct tccagagcgg
    19141 tgccaatatt cgccgcgata tcaatacgct tgttatcacg ggaataggcc agctgcgcgg
    19201 cagcctgcgc ctgttgtttt tggcgtttat ccgccagcgt ctgcgcaacc tgataataac
    19261 cgctcaccgc gtcattcggg ttaatcgcca gcacgccgca ctgggcgtca agcaccgccg
    19321 gttgcccggc ataacgggca atcgcatcca gcggcaggcc gctcagtacc gggatcgccg
    19381 aggcgcgggc cagaatcagg gtatgcgagg tgcgcccggt tttctccaga atcatgcccg
    19441 caagattttt caaatcaagg ctcaaaaact ggcttggggt taaatcttca gccaccagaa
    19501 tggtcggttt ttccagcacc agattgttgc gcggcttcag ttccggccag gtgatatgca
    19561 gcaactgttc gctaatatcg cgaatgtcgc taacacgttc gcgcagataa tcgctggcag
    19621 aagcagaaag cttggcgcaa acctgctcca tattgctgat gatcgccgcc cccagtccct
    19681 gatgctgttc tgtcatcagg cgacggatat tgcctgcaaa ttcatcatcc tgaatcagcg
    19741 acaaatgggc gctgaggata gttttgcttt cgccgtcacg ctcacgcaat tgctggttca
    19801 gttgctcggc aagcgttgcc aggctgtgct ccagccgggt ggaatcttgc acactggcgg
    19861 ggattgcccg ataactgtcg aggctgtcgc tttgtaacag ggtcagcgta cccacgccga
    19921 cgccgcttgc cagcacattg ccgtacagta aatccgggtt aaggcggctt aatgaacgcg
    19981 gcagcggatg cgccgtcagt tctgcctgcg taggctggac gctgtcgctg tcgataaagc
    20041 gcacctggat gtactcttcc agcacgcgcc gcgcctgctc ttcatcgctg ccgctaatgt
    20101 tcaggctgca actgtcatta aatagggtgc cggtgccaat cagcgccagc gaacttttcg
    20161 catcggcctt tgcgttctgg cgatggttaa taaaggtgac ttcactttgc cactggctgc
    20221 actgttcttt aagttcccac gccggacgag catgcagacc gttaggcaaa ggacagagaa
    20281 attgaatcgt taacataacc gctccgggtt aatcaggaga tcatctgaaa tccgtcagtt
    20341 gaacaaccgt ctcacgggta agacgttgaa taagggcaga taaaagttgc tgcatctgca
    20401 aaatgtcgcg gcaatcggca atcgatgccg cacaatgtcc gtggcgcgtc gctggcccca
    20461 tcaccaccgt gggcacgccg gtgccggtta aatgcaccgc tccgccgtcc gtgccgccgt
    20521 tgctgaacat atccgcctgc aacggcacgc caatttctgc cgccacggtt tcgatccagg
    20581 cggtaagttt tggcggcgca atcagcgact tgtcgcttaa caccagcatc ggcccgttac
    20641 caatctggcg atggttagcc gcgctataat caaagttttt cgcccagcag gcggtatcaa
    20701 gcacaatggc gacatccggc gacaccgcgc gggtggcagt ttgcccgccg cgtaatccca
    20761 cctcttcgct ggaacttgcc accagccaca cctccgcagg cagttcggcg tcgtgcagct
    20821 cacgtagtaa cgtcaccagc agatagcaac cgaggcggtc atcaaaggct ttccccatca
    20881 cccgctggtg agggagaacc tgaaaagttg tatcaaaggt gacgcgatcg cctggacgaa
    20941 ttcccgcctg catcacttcg tcataggagc gcgcaccaat gtccacgcgc atggcgctga
    21001 cgtcattccc ctgccggtcg ccgtcaagca ggcctggaat tttgcactct tcacgggtgg
    21061 tgatgcgcac cggctgcagc tggcgggcag ccatgcgcac gttgccaacc ggcagcacgt
    21121 caatagcgcc ttcgcgggag atgctgcgca ccataaatcc cacttcgtcc atatgggcgc
    21181 agatcatcac cttcggacct gtcgattcat tgaggcggat cagcaccgat cccagaccat
    21241 caaatcgcac ttctttttgc aggcgatccg cttcttccag taggatctgc cgcacttcct
    21301 gttccgagga ggcgatcgca tctgcctcgc tcaacgcttt taatagcgat aaatccatta
    21361 tgcctctccc gtgagcaaca ctgttttcgg catggcgtag agcacttctg cgccttgcgg
    21421 ggtaaccagc acaacatctt cgatgcgcac gcccccttgc cctggcaaat aaatccccgg
    21481 ctccacggtc agcaacatgc ctggctgtag cgtcgtggtg tcccgcggtg aaaaacgcgg
    21541 atcttcatgg acttcaatgc cgatagcgtg accagtgtta tgaccgaaat agtcgccaaa
    21601 tccagcctcg gtaatgaccc ggcgcgcggc gtcgtcaatc tgctggcagc gcacgccggg
    21661 gcgaattgca gagattgccg cgagctgtgc ctgcaggaca atctgataga cgtcaaacag
    21721 cgggtgagat tcggcgctca ccccttcgcc attcaccagc aaggtgcgcg tcatatcaga
    21781 gcagtagccc tgatacagcg caccaaaatc gagagtgaca aactcgcccg ctgcaacaat
    21841 cttgtcgctg gctttgccgt gcggcagcgc cccgcgccag ccgctggcga caatggtgtc
    21901 aaaagaggct ttttctgcgc cctgctggcg cataaaccac tccagttcag cggctatctc
    21961 gcgctcgctc atccccgcct gaataaagcg gcgaatatgc tccgctccgc gatcggcaat
    22021 cccacaggcg aggcggatta tctccacctc ctctggcgtt ttgatttgcc gcagcacatc
    22081 cggcgtggcg ctaaccagtt tcgcattgag ttcagacttc cagcggtgcg cggtttccca
    22141 actcacctgc tggccttcaa aaccgagcgt ttgcaactgc tcatcggcaa tgatttgatt
    22201 gacgatagtg gtaagcgtgt tcgtcgcgtc aagcaaatgc agctggtagc cttgcgcacg
    22261 ggcttccacc tcaacgaaat agcgcgaatc taccagaatg tgcgcacttt cacggctaat
    22321 aaccacatag cctgatccgg tggagatccc cagatgcggc tgtttgttct gccgtgagga
    22381 gagaagcact gcatccagtt gttgcgcctt aagccagtcg cgcagcgaag cgagtaatgt
    22441 catctggatt ttccttctta caggctatcg attaacaatt tgcctttacg gaacatcatc
    22501 agacgcagga acaccaccat cagcgcagtt atgactgccc ccagcgcgat ccccgccata
    22561 tagacgccca ggttagtgac cagcggccat gcccagatag cggattccgg gaaccattgc
    22621 actgcgccca gccagacagc ggcggttgag ccgacaatcg cgccgaccat atacgacgga
    22681 atggcggtga tggggctttc cagcgcaaac ggaatcgcac cctcactaat ccccataaag
    22741 gcaaggaaca tcgcggtctt accctgcgga taaagttgag cgttgaacag gcgtttgccg
    22801 gttaaacgac ggtcaataat ggtcgccaga cccagaccaa tcggaggaat aacgatagcg
    22861 atagaacgag cggtgaccgg taatacgtgg tcagtggtaa agctgaaggc aacgaaacct
    22921 gcggctttgt tgatcgggcc accaaggtcg attgctgtcg ccgcggcgat ccccattgcg
    22981 tacatcagcg cgcccttctc acctgcggca gtcagcacag tacggatacc gccgttgatc
    23041 cagccaccaa agggggtgat gacgtagtac atcgccagca tgacaaaaat ggcggaaaga
    23101 atcggtagca aaaatgtggt tttgaacgct aacaggaaat ccggtaactg gattttctgg
    23161 ttcatccact tcacaaggta acccgcgacg atagaaataa tcagcgcacc aatgaaggtt
    23221 gacggcaccg gcgaagaggt cgcccattgc atagtgctgg gatcgaagtt cagcagttgc
    23281 gtcggctggg tagacatcaa cccgccgata aaaccagccg ggaacgccag tttgccaccg
    23341 atagagttcg ccacaaaagc agcaaacatc ggaatggcaa aaccaaacaa cacgccgccg
    23401 aaggactgcg acagccaggc aaatttaagt aaggagaggt cgaaaccgga gaatttcccg
    23461 ctattaagag catccatgat gccgatctca gcaggaattt taagccagct ataagcaatc
    23521 agctggctga aagcgaggat cacaccgccc ataattaagg tcggcaccat gcgtgaaatc
    23581 cctgacatca catgctgcgg gagttcaccc cagaagctgc ttttagaggc ctgcggattc
    23641 ttaactgccg ctgccgcacc ggatgcgcca ggcacaacgg ttgcactgcg ttttttaatg
    23701 gccataacaa ttatccctgt cgaatattac tgttgttcag aggcaatcat ctcttcgatt
    23761 tctttgatga tgcctacagc gtttttaatt gcgtcctgca aagtgatttc ataaacgtcg
    23821 cgtgattcga aacgttcgtt atcttccggg gtaactgcca cggaatggat gatgatggtc
    23881 gcttcggcga tatcctgcgc cgtcaggcga ttctggatgc cgtccgcgcc ctgggtttca
    23941 attttcactt cataaccggc ttctaccgcc gcttcttcca gcgcctgagc ggccataaaa
    24001 gtgtgagcca ggcccatcgg gcaggcacat aaggcaatca gtttcttact catcgttaat
    24061 tcctcgttca gtgatagttg acgagtgaaa ttatgaacgt gcaaaacaaa tcgccgttac
    24121 cagacaaata atgcatttac tggagaatta atcccgctgt atggaagtgt gacgcaggtc
    24181 ggccttgtga ggggacacaa agaaaaattg aattaactgt atgatttaaa agattatcgg
    24241 gagagttacc tcccgatata acaggaagga tttacagaat gtgacctaag gtctggcgta
    24301 aatgtgcacc ggaaccgaga agacccggat tgttatggac gatgagatac accggaatat
    24361 catggacata ttctttaaag cgccctttat cttcaaatgc ggcacggaaa ccagaggctt
    24421 tgaagaactc aaggaagcgc ggcacgatac cgccagcaat ataaacccca ccaaaggttc
    24481 ccagcgttaa tgccagattg ccgccaaaac ggcccataat gacgcaaaac agcgacaacg
    24541 cacggcggca atcggtgcag ctgtcagcca gcgcgcgttc ggtaatatct tttggcttga
    24601 gattttctgg caggcggttg tcagctttca caattgcgcg atacaaattc accagcccag
    24661 ggccagaaag cacgcgctcc gccgaaacat gaccaatttc cgcacgcaat atttcgagga
    24721 taatggcctc ttcttcacta ttcggcgcaa aatcaacgtg accgccttcg cccggcaagc
    24781 ttacccaacg cttatcgaca tggaccagat gcgcaacccc aagccctgtt ccggcaccgt
    24841 aaaccgcaat aggtttacct tctaccggtt ctgcgccacc aaactgaatc agatgctctt
    24901 ttttcagcat cgggatcgcc attgatacag cggtaaaatc gttaataatt tccagatggc
    24961 taaaaccgag attctttttc atttcggcaa ttgagaacgc ccaggtatgg ttggtcattg
    25021 ccacccagtc accggtaatt gggcaagcga tggcaataca gccgtctttc acctcgacct
    25081 tatgttcttc aagataaacg cgaatgaccg cttcgaggct ggggtaatca agccctgaat
    25141 aggtcttagc ctgcgagatt tcaccactgg caatatcaca cagagcaaga cgtgcgttgg
    25201 tgccgcccac atcaccgact aatgcatact ttgtcattct tcaactgctc cgctaaagtc
    25261 aaaataattc tttctcacac tgtaaatacc tgggggcata acaacaacgc cggaaaggca
    25321 ggctccctgt aaatatcgat ctgggtcaca caattacttt atcgtttcag caccaattgc
    25381 agcgatgcct ttttgcaagc tgggcaaact aagtatctga ccccgcataa ggaatagaac
    25441 atgctccatc cgcgagccag aaccatgttg ttattatcgc tccccgccgt ggcaattggg
    25501 attgcgtcca gtcttattct gattgtggtg atgaaaatcg cctcggcatt acagaatttg
    25561 ctctggcaac gactgccggg aactctgggg atagcccagg attcacccct ctggatcatc
    25621 ggtgtattaa cgctaacggg tattgcggtg gggttggtta tccgtttcag ccagggtcat
    25681 gccggaccag accccgcctg tgaaccgctg atcggcgcac cggttccgcc ctctgcgcta
    25741 ccaggactta tcgtagcatt aattcttggt cttgctggtg gcgtcagcct ggggccagaa
    25801 catccgatca tgaccgtcaa tatcgccctt gcggttgcca ttggcgctcg tctgttaccg
    25861 cgcgtcaacc gaatggagtg gactatttta gcctctgccg gaaccatcgg cgcgctgttt
    25921 ggcacacctg ttgcggcggc gttgatattt tcgcaaacct taaatggcag tagtgaagtt
    25981 ccgctatggg atcgtctctt tgcgccgtta atggcggcag cagctggtgc acttactacc
    26041 ggattatttt tccatcctca tttttcactg cccattgcgc attacggaca gatggaaatg
    26101 accgatattc tcagcggtgc aattgtcgcg gcgattgcca tcgcagcagg gatggtcgcc
    26161 gtatggtgct taccacggtt gcactcaatg atgcatcaaa tgaaaaatcc ggtgctcgtg
    26221 ctgggtattg gcggatttat tctcggtatt ctgggggtta ttggtggacc agtttcgctg
    26281 tttaaagggc tggatgagat gcagcagatg gtggcaaatc aggctttcag caccagcgat
    26341 tactttttgc tggcggtaat taaacttgcc gccctggtcg ttgctgccgc cagtggcttt
    26401 cgcggtgggc gaatcttccc ggcagtgttt gtcggcgtgg cattagggtt gatgctgcat
    26461 gagcacgttc ccgccgtacc agcggcaata accgtttctt gcgctattct cggcatcgtg
    26521 ctggtggtaa cacgcgatgg ctggttaagt ctttttatgg cggcagtcgt tgtacccaat
    26581 accacattgc taccgctgct ctgtatcgtc atgctcccag catggctgtt attagcaggt
    26641 aagccgatga tgatggtcaa tcgtccgaag caacagccac cccacgataa cgtttagcaa
    26701 aaaatgctct cctttatgat ttaagagtta tggcgatgat acggggcttt acctccccgt
    26761 aatattgcgt taacaggccg ctgacggctt cgatcgtgaa ggagaataac aatgttcagg
    26821 tcactgtttc tggcggccgc cctgatggca tttaccccgc ttgcggcaaa cgcaggtgaa
    26881 atcaccctac tgccatcaat caaattacaa attggcgatc gcgatcatta cggtaattac
    26941 tgggacggtg gtcactggcg agatcgtgat tactggcatc gcaattatga atggcgtaaa
    27001 aaccgctggt ggcgtcatga taatggctac catcgtggct gggataagcg taaagcgtat
    27061 gagcgtggct atcgtgaagg ctggcgcgat cgtgacgatc atcgtggaaa aggccgcgga
    27121 catggacacc gccattaaac acattcgcca atggagcaca atgcctgatg cgacgct
//
LOCUS       DAEVZA010000060        26980 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEVZA010000060 DAEVZA010000000
VERSION     DAEVZA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.060"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            13..180
                     /locus_tag="LAF92_002554"
     CDS             13..180
                     /locus_tag="LAF92_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBI9791543.1"
                     /translation="MMKSKSKDVTLAAIYALGEGRCQAENITRELHRLSQSDDMEIKI
                     AAIKALGRIYR"
     gene            complement(252..914)
                     /gene="excA"
                     /locus_tag="LAF92_002555"
     CDS             complement(252..914)
                     /gene="excA"
                     /locus_tag="LAF92_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HBI9791544.1"
                     /translation="MKAKKETTDRFPTWWLFYYVLRKAYFFLGIPFFLFCALGFTEML
                     CSDRYFGNKVEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGIPVAE
                     VF"
     gene            complement(985..3153)
                     /locus_tag="LAF92_002556"
     CDS             complement(985..3153)
                     /locus_tag="LAF92_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HBI9791545.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSDPTLIGSMFWVFNNIIAVLAIVWCIFISIRHVVRSGHQGQVFSV
                     GRDIVGILSFVAGFLMIVPTGNGWSLAQLIMLWGASIMGVGSANVMVQVAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTAKSSTSLMTESAKTASGNYT
                     VTISNGSGICGTASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQKAADEYERAVQKSLPTDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYR
                     TQLQNSSYTPPLGTVVSSSEQRMANAQDPKSALSEIMGPLVSLTNRIATETSGTGTTS
                     AQINPLIKMKNIGDITMVSAEGIWTIYTTARVVVAGGKDSILVKFFNSLTGAASMLTA
                     LFEALAPPVYFLLFLMFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAA
                     THLGTSEDRGSRAAYGYIYLIDSMIRPPIMVFGFFFASVAIIAVGTILNALFGAALVN
                     VQFNSLTGIFSLAGFLLIYARICTTTVAAIFALQAYLPDHVINFLGGRDGANTLGSMA
                     NSVKEIFIGGSRNIRHTPGMKTDRLKDNTKGSDDKDGIKG"
     gene            complement(3250..3834)
                     /gene="traX"
                     /locus_tag="LAF92_002557"
     CDS             complement(3250..3834)
                     /gene="traX"
                     /locus_tag="LAF92_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HBI9791546.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRRIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            complement(3863..5065)
                     /gene="traW"
                     /locus_tag="LAF92_002558"
     CDS             complement(3863..5065)
                     /gene="traW"
                     /locus_tag="LAF92_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HBI9791547.1"
                     /translation="MQRKTLLAALIAILNGTAYQAHAYSVTVVASRPVEQQVVPRMEA
                     IKGVLSDILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            complement(5032..5646)
                     /gene="traV"
                     /locus_tag="LAF92_002559"
     CDS             complement(5032..5646)
                     /gene="traV"
                     /locus_tag="LAF92_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HBI9791548.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            complement(5646..8690)
                     /gene="traU"
                     /locus_tag="LAF92_002560"
     CDS             complement(5646..8690)
                     /gene="traU"
                     /locus_tag="LAF92_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HBI9791549.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWALSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            complement(8780..9580)
                     /gene="traT"
                     /locus_tag="LAF92_002561"
     CDS             complement(8780..9580)
                     /gene="traT"
                     /locus_tag="LAF92_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HBI9791550.1"
                     /translation="MQHSIKNLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLVKLIRRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTEPLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            complement(9564..9752)
                     /gene="traS"
                     /locus_tag="LAF92_002562"
     CDS             complement(9564..9752)
                     /gene="traS"
                     /locus_tag="LAF92_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HBI9791551.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            complement(9816..10220)
                     /gene="traR"
                     /locus_tag="LAF92_002563"
     CDS             complement(9816..10220)
                     /gene="traR"
                     /locus_tag="LAF92_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HBI9791552.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLASC
                     IVSIVIGAVLVAGPEMMSRSQKQLGISSISIG"
     gene            complement(10271..10798)
                     /gene="traQ"
                     /locus_tag="LAF92_002564"
     CDS             complement(10271..10798)
                     /gene="traQ"
                     /locus_tag="LAF92_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HBI9791553.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            complement(10798..11502)
                     /gene="traP"
                     /locus_tag="LAF92_002565"
     CDS             complement(10798..11502)
                     /gene="traP"
                     /locus_tag="LAF92_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HBI9791554.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAIVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            complement(11502..12791)
                     /gene="traO"
                     /locus_tag="LAF92_002566"
     CDS             complement(11502..12791)
                     /gene="traO"
                     /locus_tag="LAF92_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HBI9791555.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            complement(12794..13777)
                     /gene="traN"
                     /locus_tag="LAF92_002567"
     CDS             complement(12794..13777)
                     /gene="traN"
                     /locus_tag="LAF92_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HBI9791556.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNT
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            complement(13788..14480)
                     /locus_tag="LAF92_002568"
     CDS             complement(13788..14480)
                     /locus_tag="LAF92_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HBI9791557.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            complement(14477..14824)
                     /locus_tag="LAF92_002569"
     CDS             complement(14477..14824)
                     /locus_tag="LAF92_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBI9791558.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            complement(14842..18609)
                     /locus_tag="LAF92_002570"
     CDS             complement(14842..18609)
                     /locus_tag="LAF92_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBI9791559.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADNSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYINKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILIDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESLASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAATTASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSTYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTDTVE
                     PAVRDDPTPQQPVQSTSSSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSPETTTTT
                     PAPDSIVYAPERPDAPISLNLDEIIKSLEGEERADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATDFYGGVIELTGSDAFKQKAMQLIIEHNIKVRMKFPDQRAA
                     LEKLRKEMAVGKDTVVTHKPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            complement(18699..19250)
                     /locus_tag="LAF92_002571"
     CDS             complement(18699..19250)
                     /locus_tag="LAF92_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBI9791560.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
     gene            complement(19265..19555)
                     /locus_tag="LAF92_002572"
     CDS             complement(19265..19555)
                     /locus_tag="LAF92_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBI9791561.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            complement(19552..20700)
                     /gene="traJ"
                     /locus_tag="LAF92_002573"
     CDS             complement(19552..20700)
                     /gene="traJ"
                     /locus_tag="LAF92_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HBI9791562.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVVPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAVSITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            complement(20697..21515)
                     /gene="traI"
                     /locus_tag="LAF92_002574"
     CDS             complement(20697..21515)
                     /gene="traI"
                     /locus_tag="LAF92_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HBI9791563.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            complement(21512..21970)
                     /gene="traH"
                     /locus_tag="LAF92_002575"
     CDS             complement(21512..21970)
                     /gene="traH"
                     /locus_tag="LAF92_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HBI9791564.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            complement(22365..22949)
                     /locus_tag="LAF92_002576"
     CDS             complement(22365..22949)
                     /locus_tag="LAF92_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBI9791565.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKDPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            complement(23009..24211)
                     /locus_tag="LAF92_002577"
     CDS             complement(23009..24211)
                     /locus_tag="LAF92_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HBI9791566.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            complement(24297..25121)
                     /locus_tag="LAF92_002578"
     CDS             complement(24297..25121)
                     /locus_tag="LAF92_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBI9791567.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINNSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            complement(25272..26426)
                     /locus_tag="LAF92_002579"
     CDS             complement(25272..26426)
                     /locus_tag="LAF92_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBI9791568.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKAITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
BASE COUNT         6278 a         6538 c         6881 g         7283 t
ORIGIN      
        1 ttcttgtttg aaatgatgaa gagtaaatca aaagacgtta cacttgctgc gatctatgca
       61 ttaggtgaag gccggtgtca ggcggagaac attactcgtg aattacatag attgagtcaa
      121 agtgatgata tggaaataaa aattgctgcg attaaagcac ttggtcgaat ttatcggtaa
      181 cggcataagt tatctgttgt aatgaacaaa agccggctca tttgaaccgg ctgttatgga
      241 gggagcttat cttaaaatac ttcggctaca ggtatgcctg caatctgctc ccactcctta
      301 cgcttttcct gaatcaggcg aggaaagtcg acagggtaat catatcctcg acttgccatc
      361 gcgtgcatgg tgtttgatgc ggtttctgga tgagtacacc atgatggaat aggaaccatt
      421 ggcaggttga tgtatttcgt atgaatttcc agtgttttgt cgtctgttac agtacgagtg
      481 aaattatgaa tgtcaaaacc gataacatcc ataatgttgc ctggataaat acggacatac
      541 aacattgtgc catttttaag gtcaataccg aaataagctc cctgagttaa agataatcct
      601 tcatacccct tctctggaga gtagaagcct gactctttta ttgtttgtac aacttttctt
      661 attttttctc tccttgtttt tgcacgtgag tacatccata tgcctggcgc aagtaataag
      721 aaccaactac caaaagtgac aacataatct tcgaccttat ttccgaaata acgatcagaa
      781 catagcattt cggtaaagcc taatgcgcag aacagaaaaa aaggtattcc aagaaagaaa
      841 taagcttttc ttaatacata ataaaatagc caccaggttg gaaatctgtc tgttgtttct
      901 tttttagctt tcatataatg cctcccctcg tactatctat tattttaatg ccgatgggga
      961 ggttgtgcaa tatttatatt gatattatcc ttttattccg tccttgtcgt cacttccttt
     1021 tgtattatct ttaagacgat ctgttttcat tccaggagta tgtcggatat tgcgacttcc
     1081 tccaataaat atttctttaa cagaatttgc catgctgcca agcgtgttgg ctccatcacg
     1141 tccccctagg aagttaataa catgatcagg taggtaggcc tgaagggcaa atatggcagc
     1201 tacagtggtt gtacaaattc tggcgtatat cagcaagaaa ccagcaagac tgaaaatacc
     1261 ggtcagcgaa ttgaactgta cgttaaccag ggcagcgcca aataatgcat tcagtattgt
     1321 gcctacggcg atgatggcta cagaagcaaa gaaaaaacca aagaccatga tgggcggcct
     1381 tatcatacta tcaattaagt atatataacc gtaggctgcc cgacttcctc tgtcttctga
     1441 tgtcccgaga tgtgttgcag cccacagcgg cccggcggta catcctatta atacgctgac
     1501 tatccagttc ccgatgcctg tcatccagaa aataaacgga atgaatggca ggtaaattga
     1561 taaggaaaat ccagcacaga acatcaggaa caaaagaaaa taaacaggtg gtgcaagtgc
     1621 ttcaaacagc gcggtaagca tcgaagcggc acctgttagt gagttaaaga attttacaag
     1681 aattgaatct ttgccaccgg caacgacaac tctggccgtt gtatatatag tccagattcc
     1741 ttctgcactt accattgtta tatctccgat atttttcatt tttatcagag gattaatttg
     1801 agcactggtt gtgcctgtgc cacttgtttc cgttgcaatc ctgttagtga gactaacaag
     1861 ggggcccata atttcagaca aggcagattt cggatcttgt gcatttgcca tgcgttgttc
     1921 actggatgat actactgtgc ccagcggtgg cgtataggag gaattctgca gttgcgtcct
     1981 ataggcactc atgaccgcac tgaataagtc agtatcacca acttctccca gtgatgacat
     2041 tgaagttaca gctggagctc tgtcagccag ctctgccaga cgctggttag cagtggcaaa
     2101 ggtctgatac catgcgccaa gcgttaccca cccgtaggtt gtgagataag atttaagagc
     2161 ctcttttctg gattgctctc cgttatcagt gggtagcgat ttttgcacag cacgttcgta
     2221 ttcgtcggct gctttctgaa ttcgtgtctc gatatccggc agtgttccgt tgccagaatt
     2281 tcttttttcc agaaatgttg tcacaaactc actggcagcg ttgtccatgt cgctaatcat
     2341 attatccatg gctgcacgtt gtgctgagat aacaccgctg tattcgtttt tactgaaggg
     2401 attaaagaat tttccaatcg tcgactgatc tgttgttccg ttcccttcta ctgacaggct
     2461 ggcagtacca cagataccgc tgccattgga tattgtgaca gtgtaattac cactggcggt
     2521 tttagctgat tctgtcatca gtgaagtcga ggatttggcc gtctgattaa aatcatttag
     2581 ccctgcgtta accgcatact tacacagttc catttcaaaa atgccacgcg ctgctgttcg
     2641 tgtggatgcc tgaactggct gtacagtcat tgaataacca tttgcgatat tatcagcggc
     2701 aacttgcacc atcacattgg cggacccaac ccccattatc gatgcgcccc ataacataat
     2761 gagctgagcg agactccagc catttccggt aggaacgatc atcagaaaac ctgcgacgaa
     2821 actcagtatg ccgacaatat cgcgcccgac gctgaatacc tgtccctgat gccccgaacg
     2881 cacaacatgg cggatactaa tgaagataca ccagacgata gcaagtacgg caatgatatt
     2941 gttgaacacc cagaacatgc ttccgatcag ggtggggtcg ctggttgaca gtggattggt
     3001 gaccacatca ccgaatatcg tgaccagcgc ctggcgggac agatcgtcag gattggtggc
     3061 ggcagacaca atatcctgat aggtcactga agccattgcc ggcagtgagg atatagcaag
     3121 gcctgcacag agtgcgcgaa gtaaaatttt cacagtaact ccggaataga attgcgtatg
     3181 ggcaaaggcc acacaggatt taattaaggg ggatggcgac atgccactgg tactggcatc
     3241 tggacgtgat cagtattgtt tactggttac ggcaattaat gtggcattgc gccagccatt
     3301 gcggtcgttc agaaaatcac ggaaagtccc ctgctcgggt tcgctgactt ttcgttcatg
     3361 caactgccag aggcggtatg taacaatcag tgctctggaa gcacaaagag cgacgcctga
     3421 cagtatcaac acaagagtgc tgatggcgcg taccagagtc gtaagaggta atgaagaggc
     3481 ggcaaagagc ataccactga gaaacaaagc gatggccagc agactccagc aacagaatcg
     3541 ccatactgtt ctgcgccggc ggaaacgacg atccagatcg tctactgaca tatggctggc
     3601 ctgcagagct tcttcccacg tgaggtggag ttgcgccttt ttattattta cctgacgaaa
     3661 tctgtccagc ctgcgttttt gttgttccag catatgctgt acagaattcg ttatcaggcg
     3721 agtctccatc agcggaacaa aaatgttaat aatcctccgg gtggctatgc gggttatgga
     3781 tggtgatttt ttgacgtttt tcgtctggtc tgaattttcc ggtaacttat tcattttttc
     3841 agtcctgttc tgacgtcagc tttcaacgtg cgtttgcctg attgattgtt ttctcaaggc
     3901 taccgagttg tttttcataa tactggcggg cagtcagagc cagttgctgg ccagagataa
     3961 cattaccctg gcggatctgt tctttcagat cgttcagctg ccagttcatt tgcgcagtga
     4021 ttcttaccag ttcacggagc aggttatccc cctgcatttc ctgtaaatcc accaggtatg
     4081 ctgtgttggc gtagcgccgg ccagcctcaa aagcttcaaa ctcacgggta ctcatatatc
     4141 cccgtgcttt tgcttctggt gaagccacct ggtcgaagta agcggccgct gagtctgact
     4201 gcagtgtttc agccagtgct ttacgtgtgc tttcattagg cgtgctgtca gcaatcaacg
     4261 tcagttgggg attcgacgca gaatcaatga tgccgttgta ttcgttctgg agtccgacgt
     4321 aggttcgccc actctgggta ttcacttctc ctttacccag agcacgaccg gcagaggggc
     4381 gggaagtgtt tttcatgtaa gccgtcgctg catctatttg cttctgatcc catgtcagtg
     4441 ccgcaggtgt gtccgctgta cctgcgccgt ggtaaatcga gcgtacctga ctgtctccac
     4501 ctggtatttc gcccacagaa ggacaaacgg cggttcctcc aaaacgtgca tattctgctt
     4561 ctgtacagta actggcgtgt atggccgcgc catcataagc ttcccgcacg ggagagtttg
     4621 ctgcgctggc cagacggtcg cggatgctac ggttgctcac accgccacct ttactgagtt
     4681 ttgaggctgc ggatgctgag gcacttttgc tttctgttgc aatccctgat gcggattcac
     4741 tgcagatgga gtctggcacg gtgaagcttt tccgtgcctc ctcgaggcgc tgagtttcat
     4801 tactgaaaat catctgctga cgtgtcgcct gtccgttctg ggcaatgaca cttgccagct
     4861 tttcagagtt ctgattgata gcggtacccg tggctgtctg ggtactcagt atgtcactca
     4921 gcacaccttt gatggcctcc attctgggga cgacctgctg ttcaacggga cggctggcga
     4981 cgacggtgac tgaataagca tgagcctggt atgccgttcc attcaggata gctataaggg
     5041 ctgcgagtaa tgtttttctt tgcattttat tgtctcctgc tcggggtgga gcacaaggtc
     5101 aggaaggctg tcgtattttc tgtcttcagg tagtggggcc agtgggaaac actgatatcg
     5161 cctggtgata taacctttcc tgttccggtt atcgatgatg ctgaaagaac gacgtctgac
     5221 gcgccgtaag gtggaagggt cagtatccat tacaggccag tcaccggcac tcagtgccgg
     5281 cgttccggga acggatggcc ataaccaggt atcatcggac atcagcgaag ccagcggtga
     5341 agggagtatc cactggatga catgctgaac gtcgaatggt gcttcatccg ggacagaaac
     5401 ctgccatatt gccgttgtta tcagctcatc tgtgctggac ggcaacaaca gccgcatgcc
     5461 tgtaaccttt ccctgtgttc tggcatccga ggcgtatatc actctgggat tgggggccgg
     5521 atgtaacctg aagacacgtg acggcagagt gaatgagaaa ctgtcacctt ttacctcaag
     5581 ttttttaaga aatacctgcc ctctggcagg tctccggcag cgtggggtga tatgaattgt
     5641 acacatcaga gattgtatcc ctgttttcgg atgagctccg aggccagttc ttcaataaca
     5701 ttgtcactgt tatgttggcc cgcccggtgc tcaattaatg acgttgcaga acctctgggg
     5761 aagttttcgg ccagaatctt acgtgcccga actgaaccta acttatttgt cagtgtttta
     5821 cgcagggcac tgtcttttgg cgatgagttc agcgcccata gctccagtgg accgacggta
     5881 aatttgagta ttctcgcaag cgtgccactt tttaccctga atactcccag aacaggaaca
     5941 ccactaccat ctggtgccgg gccttccggc attttcagga agcgtttcag cataaattca
     6001 ggcacgttga agttatccct cagtacgggg atatcttcgg gtttgtagcg cagtaaccag
     6061 agagtatttg ctgacttaag aacgctttcc gggtagtccc tcagatactg cgtactcaga
     6121 accgtgcgga tagcaaactt gcgttgctca cgctcctggg tatccagatt ttcccagatg
     6181 aagtcaatgc cccgggcgtt atgcagttca tcataaactt ttgtcttaac ctcctgatca
     6241 agttggttaa tacgtttaag agcgatttca tggtattccc gtggaagctg ctttaacact
     6301 tcatccctgt attgtggcag ggtgaagtca ccaccggcga tctgacctgc aagcaggtac
     6361 atgatgccgg ttttaagcct gcctgcaggc gttttgtcac cggcaacatt attcagatcg
     6421 acagcgatga cccgggtttc cgggttgatc ataaaccggg tatacccgga tatcatgggg
     6481 tagtcgttat gtccctgctc gagagcgcgg cggatgtagt ccagcagaag ttcgtttgaa
     6541 ccatcacgct gaacagtacc gaaaacatcc ctgagactgg tatggcccag catcgaggac
     6601 acttcgggaa gctggggcat tgcctgataa tgtgcccgct gagccgccat gatatacccg
     6661 gcattgtgga gcatatcccg gacttcgaac caggttgatc tggcccacca ggctgcatca
     6721 tgtttttcat acagtcccga gtcctgcagt gctgaatcca ccagttcttc cgttgaggcg
     6781 cgataaagtc gcggattatt ttcaccgtac tctttaaaag ccagctcgat aagctggttg
     6841 ataatttgtc gggtgtctcc ggggttacag ggttgaccgg taccggtatc gacacagaga
     6901 gcacacagca cagatgacat gaaattcttt tccggcgtaa taggcttttt agcgccgtac
     6961 atcacgtcaa aaaggttacg ggtgtattcc ggatcattgc tcagaataat accgacagct
     7021 tcgtcttttc tttccggagg aagactgtcg cgaataagct gaaccagtcc ctgagcactg
     7081 tatcccttat cgatataggc aatgaagggc aggtttttct gagcagatga tatctgtatt
     7141 tccgacaacg tattaatcag aactgattta cccagtcccg gatcgcctgg tgccagttct
     7201 gtatgtttat tttgttggga tgacgccaga ccaacctcaa atgcggaacc gtcttctgta
     7261 tgtaacatca gattcccttt gcctcgccag acagagcctg ccctgttaag cgggaaaagc
     7321 gagatggcat gtgacaacgg gggatacagg ggaaccgggc cggagcctcc ggatgccgcg
     7381 agaatggtat tcacccaggc gcgtcgggga tcgccaaatg ttgtcgtggt gccacatacg
     7441 ccccagcctt caatggccga tttcagaatg gcctggtttc gtgtgcatat ttcacgtgtt
     7501 tttccccacg tggaggccat gatggtcatt atgcagacag gttctttttc atccgttgca
     7561 gccagggtca taacggattc atacattgga cgaacggcag aaatgaagct gctgtaagtc
     7621 agaagcgttt tcttcagatt aagagccttc atcccgccgg gcatcagatc cattctgatg
     7681 cgccagggga ctgcgcgggg gacggcacgg accagttcgt taaacgtctg cagattttgc
     7741 ggaggtaaag taatcgatac cattccgtgc cagagtccgc ccgcctgaac caggttgccc
     7801 tgtgtggtca cctgagtgtt gaataactgc aaatgcaatg aaggagcatg tagtacggat
     7861 tcatcgtcag tccagcgata tcccgctggc tgggcatctt caggcaggtg gggttgccag
     7921 ttacgcggtg tgctgtgccg ttctgtctga cgacgtattt cacgcccgac ctcgtgtata
     7981 tccagtagcc ggagaatcag gccatcagat gaatgtctca gtgcctgttc cacattatca
     8041 agaaaagctt cgtggcggat tttcagtgca gataaggccc actgccaggg ggactgggca
     8101 aagcgtgctt tcggtacccg ttcagccagt cttctgacaa gctcatcatg ggcggtgcga
     8161 tcgctgttgc tgataaggtc gggtccactc cagatcgcca gccagcaacg ctcccggacc
     8221 agccatgggg acagtgttgt gactttctca tcgaccacat cctgtaactg gatacctgta
     8281 ttagccagtg agcgcttttg tggggcgacc atgtcttcga tttcttcttt ccccatgtca
     8341 ggatcacgct caaaaacgaa gctgatttta tgaccgctgt ttttatatgc cgtattcagg
     8401 ctgtcactca tgctggtgat cagcgattcc agtgaacctg ggcccgtctg gtcggatgct
     8461 tcatccattt ccctgaaagc accgctgact tcaaagacag agagatattc tccacgcttt
     8521 gtggctgcaa tatagggcga attcagccat gggcggcgtt cacgatcctg tctgtccaga
     8581 ccaattacag tacgtaaatc gcaatattca acaaaactgc ttgcgacaga gaaacgggat
     8641 agcgtgttca gacagtcttc gatagtgaag attaaacggt ttatgttcat aggtgaggga
     8701 actccggact tacaggaggg aggtttgtgc agtttgattt ttttaatgcc accccgcgct
     8761 ggagtggcat gaagaaaaat caatcaacgt attctttttt gatgaatgaa tctggtgtca
     8821 gaatcagggc aatcccgttc attttttcac gcaggggcga acgaccggaa ggtggatagc
     8881 gacgaaaaac gtcagcgaaa tcggccgggc ggaaactgcg gaatttatcc tcaaccgcca
     8941 gtctgttttg catcagtgca gagagcgctt cccgtccccg ccggacccgg aacttatcat
     9001 tactgaaaac ttcctgtaat gaacggcggt taagggccac gactgtggat ggatgaaggt
     9061 cgggtagcca caccataacg ccctgtgctg cggcataacc attcagtgaa tgccaggcgt
     9121 ggcagaaagc acataagctg taaggcgggc cgtcttcatg aaacagttcc tgctctttct
     9181 ctgacaacgg ctctgtactg gtgatttcag ctttctgtac atggcggagt gaaagacgtc
     9241 tggcagacag acgggcctgc tcaagcgccc gttcagcggc ttttactgcc gtgttgtact
     9301 tttccttttc gccggttgcg actggttcac cacgcctgat taacttaacc agatgttctg
     9361 tggccttatt aagacgaact cgtgtgtcac tgattgtgtc ggtaagctgt ctgagtggcc
     9421 atgagtcatc cagtctgaaa cggtgcctga cgttttgccg gcagcagata catcgaccgg
     9481 gcgttgttaa ttccggttcc ggtaacagtg gctcctgtgt atgaaccaca tcaatttctg
     9541 gaaagggata caaccacaag tttttaatgc tgtgttgcat gagcaggctc cgtactggtg
     9601 taacgtttca cggcttccag aatgatgttt gcgatttcat cggaacagca ttctgcaaaa
     9661 atgaattcct gatcgtcccg actgtcgtgg gcaatcacat tgccgttttc tgttcgtcgt
     9721 aacgttatgt gctccgggct ccaggcagac atatgtgcct ccttgtattg ccgaacagga
     9781 agggcaccgg ataaatgcca gtgccctgga caagattagc caatggaaat tgaggaaatc
     9841 cccaactgtt tctggctgcg actcatcatt tcaggtccgg cgacaagtac cgccccaatg
     9901 acgatagata caatgcaact agccagtccc atcccccctt gtttcccaac ttttttcagg
     9961 ccaatcaggc ccccgagaaa cagaattacg ccgatgaact gcgcaatagt cagtgatgat
    10021 gactgtgctg ttttggcacc ttgttcgaca ttgcgaacca tatcggcaag atctccatcg
    10081 gcaagaacgg ctttgggcag caacatcatg ggaatgagca gcaggaactt agtcagtttg
    10141 ctccggcatg acattacaaa cagttgtgcg tggataaatg cggcgtacag acgggccgct
    10201 acgacacgaa tacgtgacat agtttctcct taaatggata tatcagtggt ttatatgaag
    10261 gtgaaaaaaa tcagaagacg attccaatgc ttgccagcag ggcattgagg acacgatcga
    10321 taaagaccag caggacgccg gcaataaatt tgacgtttcc cttactgacg gattcactgg
    10381 cactcagtgc ggtatgtcca tcgagtccgg ccttgcgcca gatggatatc ccgttcatta
    10441 caaatccaac accggccagc ttggccagcg acagaactgc atttgctgcg ccagcagcag
    10501 cgccaaacgt ctgaggttgt acatatgcaa tcgggccaaa tgagtcagcc ctgaatccca
    10561 tttgtacgcc tccggcgtta atcattgccg ggagtgatac gagcatgcag ctggttaaca
    10621 tccccgcaat aaattttccg ggagacattt ttaccggacc ggctgaacgg gcgcgggcaa
    10681 acagggtaat catgctgatc accccgatga attctcccca ggtaaacagg aaatccattc
    10741 cggcactgaa aatactgctg gcaaagttct caatggcggt cagggcatcc atgttcatca
    10801 gcgtaacgtc cctgcactgg tgatgatggt ccgggtagtc ggatcaatcc gctggatcac
    10861 cacattgccc agcgtctgac cttctctgac agcccaggta ctaccctgcc agcgaatcca
    10921 ggccatacca gtttccatcg acatgatttt cattccgtta agtacagacg acgttttttt
    10981 ctttgctgga tgcggtgtgg aagaggattc agtgacgctt ttctgtgtca ttattccgtt
    11041 caggcggctc cggatatcat ccagctgacg gcgtgtttcc tgtgcgtatt cttcatcctt
    11101 ttttatagct tcgcccagct tgttaatgct gtcgtgaagc atattcagtg ttgcctgaat
    11161 tttttcatcg cggttatcca gctctgtttt tacatcccgg gcaagctggc tcatatcttc
    11221 tcctgtccgg acagtcatag tctcagtgga ctgaacggaa ggtgatgatt cttgtgtggt
    11281 ttgccaggtc tgtacgccag catccgcatc attaaacgga ggcatgcctg tttccgatgg
    11341 cgctctcagg aacagatacc atatggctgc gagcaacacg attgcacata gcccccatgt
    11401 tgctacagat atcccccaga cggaacgttt ccagaagggg gcgggtttct cttcgggttc
    11461 accgaagctg ccggtttcat ctatttctgt ttcaggtttc attatggtgt ctccggttgt
    11521 tcatcatacc gacgttgcat cacagctttt ctttgctcca tggctgtctg cattcgtgcc
    11581 tgcgagcgtt gttccgcctg tgatgtacga ttaactgaag gggaaatgcc actggaaagg
    11641 gaagatgacc tggcgtcgtt tgtcattacc gggtcaacaa acagaattga tacgacttca
    11701 tgctgataca cttctacctg tttatagggc tcactttctg cctggcgggt taatatctga
    11761 cttcctcttt ccgccatacc accggcaata acgcccgcaa cagcttcacc actgggcatt
    11821 cccacgcgtc cggtaaccgt gccatagtta ccctgaataa cctgcgtgtt ggcatcttta
    11881 taaagtgtgc caatactgcc cacacctcct aacactgacg gcagaatgat atgtttaaac
    11941 cagcgtgtat tcacattgct ggccacggat gacatcaggt tgtcttcccg ttgagcatat
    12001 gcattgacct taaggtccat gccgttccag ctcatccggt cgaaatggat ctccacgccg
    12061 ttacccacca gtttgactcc cggactgaac agcgttgccc ctgcccaggg gccagcggga
    12121 atggttgcga taaccttgga tgacgggtta tcagaatcca ctgcagtatc aatatatgcc
    12181 ggtatacggg tcagagcagg tatcagttgt atcccttttt ctgctacagg cgtggacagg
    12241 ctggcattct gtaatgcgaa ttgctgcgta ccattctgcc ctgttgtttc tgccggagat
    12301 ttattccaca tagtcgttgc tatggttggt gtactaccgg caggatgctg atcttttatt
    12361 cttacaatca gcttttgcag ccgctccata cgtttttcac tggctgctcg atcctcctgt
    12421 ggtgtgccgg aagcctggcg atgagcatag ttttccggtt ttgctgcagg ctgttgttgc
    12481 ttttccggag tgtccggaat atcaagcccc tgtggaagac tggcaatgaa cgtctgatta
    12541 ttgcgggctg cagctgcggc ccctcgttca ttatccgctc ttaacagttc acgatagtga
    12601 ggcgtttcgg taacgctgcg cgttccgccg cttgctgcac tgttaatgtt cacggctgac
    12661 tgcatatcgc tgttactccc tgatagtttg gaaaacgcga tatagccgcc gccaaataag
    12721 atgatgctgc ccagtccaag cagggcagcc agtttctttc cggatttacc ggcatcctgt
    12781 tcagcactca tgattactcc agttctggtg tcacgtgaac ggactggcca ttcacagaga
    12841 aagtcagtag tggggtgacg ggaagtttcc agagatgagt tccgtcggca gaggacaagg
    12901 tctgttcaaa ttcatcgcgc aacatcgccc gggaacgaac cagaaggtca tcgccgtgtt
    12961 gccagatcgt tgtatccggg acattacctg tgaatttcag cctcttaact gaagggtccc
    13021 ggggaggaat accatccaga aatgcctgta atgttgcatc atgcagggcg atcttatccg
    13081 tcgggatgga gacaactggg ctggttggtc cctgccgtgg tattcgcaga tccagacgac
    13141 tgtccatttc ctgactggat gacggcgtat ctgtttcgcc actggttacg ttcaggatga
    13201 ccggtaccga cagacctttc aggtagacag aaatatttcc ggatgcccat ggccgcaatg
    13261 gtgtgattgt caccatgttc tcgttgtact gcacatcaaa gagttttggt gccgcgttat
    13321 aaggaggatc ggattgtggc catggtgaac cgttgatatc ggtaaatgtc accacgctca
    13381 ggttgttcat ggctgtgcga acaagaggaa ggctggcacc gggggacaga ttaactgtca
    13441 gagagctgat acggggtaca acactggtga tgggcgcatt gatggcccgc tcgttatccg
    13501 ccatgagact gcgtagtgag cgaatctcat cggcggagag ctcagaatca agccttgccg
    13561 catcctgtac cagcgggctg gcctgcccgg gggcaggcag ctcacctttt accagtgttg
    13621 ccggaacacc tccggtgttt tgggaggtgt tctgaacgtg atcgctggca gaatttgtcg
    13681 tttgtggtgt gcgtgcgttc tgccatcctg cgttatctgc agacgtggcg gcgctgcaca
    13741 ccaggagcag tgtgctcagt aaataacgtg attgcatatt acttatctca ggagttcggt
    13801 ccggcgttac gggagatcat ctgccggatt tccataccgg atggtttcag tctggggtca
    13861 acacgctgga tggtgatttc gaaaacaaaa gactgttctg gcttgctggt tgtctggccg
    13921 accagacgca tacggacagg atactggaat gtccagaacc atacgccgtc ggacatttgt
    13981 ccctgtcgta ccagaacgcc agcacctgtc gtggcggtca gattcatctt ctctttcttg
    14041 atcgtctcca gaatgttaga tgcctgtaat gcattcacat atccgacaaa accttcgtca
    14101 gaaaaacgcg gagacaggga ggagatctgt gaacgatagt gcacaaaatc caggttgaat
    14161 gcggtcgcca gtgcctggct gccaaaggcc attgcatcat tctgactcca tgctggctga
    14221 cttgttggtg ccagcctgac cagacgacca ttgtctgtag cgaaatattc gcgctccaca
    14281 ttcgttgagt gccagtactg aatggcgtta ccagtaatac tgataagaag acatgtgccc
    14341 gtacataaaa gcgcttttat cagcgcaggg acattatttg cccgctgttc tgactgcttc
    14401 atagccctga ttgtgctctt cagtatttcc gggtcaggtg ccggatgtga tggcgaggat
    14461 ggctcggtat tttcggtcat aggttgtagt ccttccaggg atcaatattc cccggcagct
    14521 tttcattcac cttgtccacg accgcgtcgc agacctggtt ttccagctgc gacagaatgt
    14581 cgtcaaggct ggggaactgg gggaggttta tgctgatttt tttgattcgt gacagacagg
    14641 actgcaggga tgaggaaaca tcgttttcac gttgtgacca tgcatccgca gctgcacgtg
    14701 ctcgctcata tcctgtatta ctaccggcct gtgccgcgct acccgcacga caggttgcag
    14761 cacttgccgt ggatgaaatc aggataaggc cgagcacggc aaccagagac ttatgcatgt
    14821 tcatatggct tccttaccgg ctcatcggcc ggggctgtgg gagagatcct gatgcagagt
    14881 tttttcctgc ctgtcctccg gcttttggct gttggaccac tgctgccatg actgctcgaa
    14941 gcccaggttc cggtacaacg catcaggttg tttttccggc gtgtagacag cagcaaaaag
    15001 cggttcttcc cgggttttca gatgaaactg cataagctcc ggtgcagggc gattcgtgct
    15061 gtcagcatcg tggtttaccg cttcaccata acctgcaaag cgtaaacctt tgtcggtcag
    15121 cgcattgata accagctgac tgttgtcggc tgatggggtg gccagtgccg gatacaatgt
    15181 gccgttcagc cggaccacac cctgcagatg ttcatcggca ctctggacaa gtagcaggtt
    15241 gagttcacgc gtatccttat ccactgatcc cagaacaggt gtcagcttgt caggggctgc
    15301 gctggttatt tctgttacct gatttgcgtt ctgtggcgcg gagaccggat gtcggtcttc
    15361 accgataaac agatgtgagc cgacgttttc tggcatacgt aacgctttca tctcttcggg
    15421 ccacccctgg ctgatttgcg cagccacttt cataaaggtt tcgaccggaa tcgcctgaat
    15481 acgctgacct ttatccggat tatcatgtga taccgagatc ccgttggtaa ctgactccat
    15541 ggaccactta ttccggaaac gtgctttttg ctctcccggg aggatgaatt gttcgcggtt
    15601 ctgaagtgtc agtgtcacca cgtcaccttt ctgatggttt ttgaccagac tttccagttg
    15661 ttccccccag tagagtttgc tgccactgcg gtttttaagc tcaacaaaga aactctctcc
    15721 tttgccgggt gcatattcag cacgaccaaa attgtgcagt acggcagtca cgctctctcc
    15781 cgggcgaagt tttcctggtg gttcttcacc ttgtgccgca tctgccgttt tgccaggttc
    15841 agccggtgat gatgcaggga cggtgctgac ggttgatgct tcaggggcta ctggggacgc
    15901 cggggattgt gtggcttctt tttcctgaac agggtctggg actgctggtt gttcaggggt
    15961 attccggtta agttccggtg ttggcttatg ggtgacaaca gtatccttac cgacagccat
    16021 ttccttgcgt aatttttcga gcgccgctcg ctgatccggg aatttcatgc gcactttgat
    16081 gttgtgctcg atgatcaact gcatcgcttt ttgcttaaag gcatcactgc ccgtcagctc
    16141 aatgacacca ccataaaaat cagtggccac agcaagtgca gccagaacac tgcgatcgtc
    16201 attgctggca ccgttgacca tttccagccg gtttacccgg tcaatgaagg caggtttgcc
    16261 atccagtttg tacaggacgg ttctgtctgc tcgttcttca ccttccagcg atttaatgat
    16321 ttcgtccaga ttgagcgata tcggtgcatc agggcgttcc ggggcgtaga caatactgtc
    16381 aggtgccggt gtggtcgtcg tggtttccgg tgagggtgtg ggatcactgt tctcatgcgc
    16441 ctgggaatcc gtttttttac gcgtaaaaaa gccacgcgct ttgtcgagaa acgatgaagt
    16501 attatcagaa gatgatgtgc tctgaacagg ttgctgtgga gtggggtcat cccggacagc
    16561 cggttctact gtatctgttg tggcctgaag ttctgactca tcagcctgaa cgtggcctgt
    16621 gtccggttgc gacggcatat cactgttttc ctgcagatgc tcaggatttt cattgttcat
    16681 cagctcctga tatgtgctgt agttttcgta ttcttcgggg ccatctgact caggaaggaa
    16741 cggctctggc tgaaggggaa tgtcttcagt tacatggttg accgcctctt cccggggagt
    16801 atccggggat attccggtgc tttgtttgtc atcaggctcc gtcatcacgg tcatttcctc
    16861 tttgactgca tcaggggaag gtgatggctc ggctgattca ttcatggttt cagatgaggg
    16921 actgtcttca ggggatgcgg tggtggcagc aggggccggt atttcaggtg aagtgccgga
    16981 ttcctgttgt gactcgtttt tttccggttg tgacagttca gcttcaaatt cacgccagtc
    17041 tttatctgtg gccaccgaga caactttttc cggaggttcc gccggatgag cgggttcatc
    17101 aggggcagac gccgttgtac tttccgggga cgccagtgat tctgcagggg agtcctgaac
    17161 tacagagctt tccgtttgcg cctgaacgtt atcagcgtgc cgctcatctt tgccgttatt
    17221 ttcagtggcg tgactggtaa atgactgtgt ttcatcagca atgttactgc tggaagcagc
    17281 tggttgttcc tgctgcgtgg cggacggcag agaaggtccc tggtcatggc tgtgccctgt
    17341 atcgggtgat gtttcaggat attctgtcgg agtcatggtt ttttcctcag tgtggaccgg
    17401 tgtaaaaacg aggttgccta tcaccagtgc ctcgcgcatt tccggggtgt tactgttggg
    17461 ggtggaaacc ccgatgtgtt ctaactgggc gttgatgaca tgctgaaaag cggcccgtcc
    17521 ccggcttgcg tccaggtcat tgaaatcggt cagcccctgc gctttttcgg catcggtgaa
    17581 cgcaggaaag atgaccgatc ctcctgtcag ttctgcggct tttgttgcac tgacgatacc
    17641 tttgttaacc cggattgcat gatcgttgtc ggcacagaag ataaaggggc tttgcgtcca
    17701 cttttgccgg gcattttcgg caacggcaat catgttgcct ccatccaccg tcatcagtac
    17761 cggaaggccc gtggcttcgt gcagggaggc cgcagtggca tagccctcgg caaacagtac
    17821 cggctgaccg ttgcgcggtg tgcccagcgc aaaccagtta ccggttttct ccgaatctat
    17881 caggatgcgg gcatctttcc ccccggtgac cggaatacgc tggtaagagc gtatcgcgcc
    17941 gttacggtta ctgaagggaa taaccagttc atttttgttg tttacccgca cccccggtgc
    18001 agcctgaatg cctttccggg tcaggtattc atgtgctgtg gcctgcggcc atttgttgat
    18061 atagcggcgg gcatacgcgg cctgtcggtt gtactgtgct ttcagttccc gctccgcgtc
    18121 ctccctgcgt tgcattgaat gcgctttcag gtggagtctt gcgcggggat ctgtctgttc
    18181 tccgccggag aaggtccagg tgatcggcga attatccgcg ctgcgataat ccctgtacca
    18241 gccggcaggt ctgccgtcca ggaatccgcg atatgcgcca cttttttgtc cttttttgtc
    18301 atcggcggtc gggacacggt ggattttccc gtccatgacc ggcagttctt tcagaaccag
    18361 tccggcattc tccagaacct gggcaaactc ggtgaccgga tcactgccgt tcatggagac
    18421 gtcctgtggc cggggcagcc agcggtccag cctggacagg tccgcccccg ggcgggcgaa
    18481 ccagagtttg tgctccttgt cccagatgac gggcgtgtgc ccgttcatcg ggggatgtgc
    18541 ttttttgaga tcttctattt catctgcagg cacggcaagc caggtgcggt agcgggatac
    18601 agacggcatg agtgcttctc ctgaaaaaag acggaaaaaa accaccgccg gaagccgggg
    18661 gtggctgggg ggatagaaaa tcgggggaga aatgtttgtc agggtctcca gtctgtaccc
    18721 ttattccagc gatcctgcca gtgttcaaga tattgtgcgg ctacatccgg catattcttg
    18781 agcacaacga cattctctga attcttgcgt gcagcagcac gagagaagtt ataggagcca
    18841 gtttcaaccg tgtttccgtc aacaatgatg actttgtcgt gatggatggg gaaggcatct
    18901 acagtccgga gaggaatgtc cagcaggttg atatatttca tcgcttcctg acttgcccgg
    18961 ttcgtattcc ccttgtcatc aacaacaata cggacgtcca ctccgcgttt tttcgctttt
    19021 gccagcgcat gcattacatc cgggtcggtg aaggaatatg ccatcatcct gatggagctt
    19081 tccgcactgt tgattgtgtc aagcaccaga gcggatgcgc ttccttcagg agaaaagccg
    19141 accttcacgg atggcgtgtc gatcattgca aacgatggtg tcgtgaacag taacgggcat
    19201 gccagtagcg atacccggag cagagtaacg atagtttttt tacctgacat aaagactcca
    19261 gaatttaatc gaggccagtc cagtctcgtg gtgattccgt atggtggcga taccaccatg
    19321 gacgaccgga ggcgatggcc cctctcagaa tgctgaaaat gcgagtacag atcgtcctga
    19381 cagtccagcc tgaacggtcg agaatgacga aaaataaaat gatgccggtg caaatccaga
    19441 aggttgtttt ggagggaaag gggcatataa acagataaat catggtgaac ggcagaggta
    19501 cgccctggat gttgactggc ggtgttgcat atcgccagaa accttctctt ttcatgaccg
    19561 taactcccct ggcgggataa aaagaatcgc ttctcttcgg tcaacttcat tccggatata
    19621 catttccaga atctggtccc ggatacggcg cttctcctgc cggattatgg catcaatgtg
    19681 atgcccccac tctgcatgcg gcattccgga aagattgtcg cgaagctcat cgttaatgac
    19741 gatatattcc cggacggctt tacgaccacc gtcatttgtt ctgaccagaa cctgaaccag
    19801 aatgaactgc aacagtgaca ggacagccca ggccatcgaa tccctgatga cagggggaaa
    19861 cagcccaaga agacgtgcca gcgtttcacc aggagatttc gtatgcatgg tgctcaggca
    19921 gtaatgcccg gtgttgcctg cctgtaccgc tgcgtcggcg gtttcgttat ccctgatctc
    19981 accaacaccg ataatttcgg gattacgtcg tacagcagaa cgcagtccgg cagcaaaact
    20041 gaccacatca cgcccgattt ctgcctgatg cggtgggagc agatcgtttt catttcccag
    20101 gatatattcc accgggtctt catatgtcac gattttggcg tcggggaaat tgtccataat
    20161 gtaacggtac agtgctgaac acagcgttga ttttcctgac ccggtttcac cacatacaaa
    20221 tcccagaccg cttttgcaga ccattgcttc aagcaggtct ggttcaatgt tcatggagag
    20281 aatgtccggt atttctgtcg ggatgacacg catggttatt gagactgctt tctcctctgc
    20341 accagatgtg ccctgaatga ggtgagagcg taaccggatg cgttctccgc gtttaagacc
    20401 atagcgaccg gatgcatccc cgttaatctg tatcgtcctg tcaaccggat tgcctgccag
    20461 aacgcgggga acgacttccc gaccgaacag ttcatcaatc agggaggaca tgagtgtggt
    20521 tggtaacgtt gcactggaac aacgcacccg tcggccaaag cgactgacag atacaggtga
    20581 cccaccggtc agatccacat cactgacttt atgtgcggca caccagacaa agaaatgccg
    20641 taattcatct gcagtgaaac gctgaaacgg aaaaagacca aactcatcag gaatattcat
    20701 cgggagcctc cttcagtgcc tataagcgga cgccagtgag atttctgaag attgaattcc
    20761 gcatgatttt taaggcgacg aacacgatct ccagtgacca gttttttccc gtcacctgta
    20821 acggtttgca tttgatcaga aacatccgga cgggtgatca tgccctgacg ccagagaagt
    20881 gagtacagca tcatgccccg gtaatcacgg gtgagggttt tctggttggc ttcaagcgtc
    20941 aggtcggcgt tttgtcttcc ttcctgccat ccctgccgta atgcggtttc ccacactttt
    21001 cgttgcacgc tgtcttctgg aacgacgctg ccttctgtac ccggagtggc tgttgttgaa
    21061 agcccccgga gaagccagtc acgccagccc ggtggattgc tgacaaagcg ttcagggcga
    21121 atgatggtcc agacccggga agaggtccgg atttgatctg gcgtgatatg agctacgtcc
    21181 tgtgcttcat caataaccgg tggtagccag ccttccgggc taatcagtgg ccggaaatcg
    21241 tataaggcat taagtgtgct ttcccgggcg ttgagtgcct gaatcagctc ccatgaacgc
    21301 tgagccttgc caccacgaaa tccaagcgtt ttacctgcgt cggtcagcat tttccagcgt
    21361 gtttctgaca gactatccgg cttttcactt cgtggtttaa gccatgcatt aatgtcaggt
    21421 ggtggcgtat tgacaccacc tggcgagatg gtaggggaag aagaacaacc agtaagcagt
    21481 attactggca aaatgagaga ggtgagtgct ttcatttttt ccggttctcc ggctgcatga
    21541 agctgacctg taacaagccc ggatattgat ggattgttgc tctccaggct gtctgggctt
    21601 caataagtct caacgtattg gaaaacgtca tatcccggac gtgaaggttg actggtaacg
    21661 gcaggcggac accattgtag ttaaactgca tccccctgac tctggccagt tcgttcagta
    21721 attcaattgc atcaccttcc cagtcaaagg aaaggcgatc gctgttagct gtcagaagat
    21781 gagaccccgt tgaaaccgga agttggccgg aaaacacacc tttgtcccat aatgtgtact
    21841 gagtgtgccg ggtaaccgcc acatcagagg cagcaccgta gttacgcgga acaggagtat
    21901 gctgagaagg ggagttgcag ccagccagaa aaagggcggc aagacacccc gcaggtataa
    21961 tacggttcat aataattcct gagcagttaa atcagaatgg gatataccgg tgctccggta
    22021 cagacaggat gatgtttgtt tccggctata cttgcgttac cggaaataaa tgccagtgtg
    22081 gccaggtaaa tagcctcata gtttactgaa ggtgattatt cctgatactg gctggttgat
    22141 gtgtcgtcag acataaaacg aatatgagcg taatctttaa cgctccgcgc cgaatcgatc
    22201 catgatggat gactcgacga tttatgaatc gaacgtaccg gcgcttgtcc ctgaaacagt
    22261 tcccggccct cgtccggatg acctgcaatt cagacaggca catgtgcccg tacactcttg
    22321 tatcttactg agtatatcag aatttatgta aattcaaata tcctctatgc ctctttgttg
    22381 tattttccgt caagtatgag ccgggtacca tcgtaatgca tgatcagggc atcaaggtac
    22441 gccggtttga atttcttgcc gagcctatct ctggccagaa atgataaaca atgattatgt
    22501 gtcattatta ctatgttcct tttatgagat tctcttgcga ttgattcaag cgtcttataa
    22561 agatcattat tgcagtctga taaactgtcc ataacgacgg ggtctttacc ggagaagaat
    22621 tttgcagttt gtatggttct taccgcattg ctggagtaaa tatcatattc actgaagatt
    22681 gttgcgaact taataccttc ctgctgtgct ttctctgtac cggtaatagt gattccgctt
    22741 ttatccgaat agcatggcat atctgaacgg tcgcaacgtt ctccatggcg gaaaaggaaa
    22801 ataacggtgt tatcagcatt aatttctttt gcctgttcta gggtgatatc gggaagcgag
    22861 ctttttgctt ttgtcactgc agctaccagg aaaacagaaa ataaaagaat gaatgctgtc
    22921 tttgaataag aagaacgtgg tttcatcatg ttatttaaaa tgaatataaa aatacccccg
    22981 ggtttagtgc cgggggcggg gacagtaatc agaaagtgaa ctgaagttgt gccattgcac
    23041 cgtaatcgtg gtcagttcct accaggccgg aaacatcgat atgtacgacg tcaaatggag
    23101 agataccaac gccagcggtg aaggcgcttc cggagtttga tgccatgttc tgacgataac
    23161 cggcacgcaa ttgcgcccat ttccatgcgt taaattcagc accaacactt gcaaactgac
    23221 gtttgctgtc agaagtgaag ccactggcag gagtcagatc aacgtcaaga gcagtggtaa
    23281 acaggtcatt atgccaggat acaccggcgg ttgcctgtgg acgaactttg aaggtttctt
    23341 taaagccatt gacgacttta gtatcaatac tgcgtggaat gatgttctgg gcaacaagac
    23401 cgacagtcca gttgtcattc aggtctgtat aagcaccaat atcggcgttg aatccgtttt
    23461 tcgtgttgtg gtacttgtca ccgtcaaaat cgtctttgtc gtaatcacga accgttacgt
    23521 tgtagttaaa caggtccaca cgctgatatt ttggtgtgac gccaagagac catttttgtc
    23581 cggccgtttc cagttctttg gcaaatgaaa ttccgacgtc ggtaattaca gctgcacgcc
    23641 cgaatgcgcg tgaggtcagg gcattcttat cgacgtcggt aattgttcca ttggcaactt
    23701 tgtccagata atccaggtct gcatcgttta cttttccgtt tacgctgaca gttccgtaag
    23761 acttgaccat aagggcaaac ggcagagtgt catttgccat ggctgctaca gctgatacac
    23821 ctacctgagc atctgcattg atattgcgga attcctgcag acgatgttta agatttgccg
    23881 ctgcctgttg tacgccatgc tggttatcta cagcactgtc aaacaaatcc cagtcatctt
    23941 taacatcatc agctttattt gatacatcat caggatcagc aacttgtgcc ccaacagacg
    24001 gcagaagaag gctgaaatca tcattgctgt tgtgctttgt cagtaaagcc gggtttgcca
    24061 gaggtgccac accataatgg gaagatgcta ccccggtgcc acccattgca tcgttacgcg
    24121 cttcaaaata tgacgttgct gctgcagcgt taaaggaaaa taatacagca gatgcaatga
    24181 tggtgcgggt aatatgattt ttcttcatca tttaatttcc tgtttatcag aatttagaca
    24241 aaacaacgct atgacacgga tgtgccgtaa atttttactt tggtactatc tggtatttac
    24301 cagggtaata aagacgtatt aaataagcta acaggtagta tttgccctgt aataaggttt
    24361 ccggcaaggc caggtctcaa cgtaacgcgt cgcaaagaca ttgttgtatt ctgcctggtt
    24421 gtatattcag ttaaccattt tcttttttca tcgttcagaa gtgttggtga taatgatagt
    24481 gccggatata ttacagagcc atttatttct ttttcctgtc tcaatattac cccgcaatat
    24541 tctcttttcg tgtttcctgc gaaggggaaa ctatgccaga aaataacttc agtgttgctg
    24601 ctattattaa taagcattct gaaattatca gcagtatctg gccattcacc ggacaacgct
    24661 atcggatagt tttcatggcc attcagccag tccggattga gattaaaaca ttcagcaaga
    24721 tgttcgagag cgtcagctga aagatagtct gcaagtctgg ccgggtcctg tataaccgtg
    24781 gagcggatat tccatggcga cagcaatgtt gccagtaatg gcgcatttat atcatgcgac
    24841 agcatgatgt attcaattct gcttattatg tttcctgtgg tgttatccgc aggcataaac
    24901 atattatgaa gagcatcctc aagcaggatt cgggttaaat cagcggtgga tatggctaat
    24961 tgttcgctca ttatccccag aacttctctt accccttgtg ggtaacggac ctgcagaggt
    25021 gtcgggttta tctgacgacc gttgatgagt ttttcgccga ttgctttttt gtccagcgag
    25081 agcatcattc gggccgcaat accggtcgta ttcagtctca ttgttcctcc gactgaaaaa
    25141 gtcttttcag ctcatgtggt tattgtatct tcggtgtttt ttctggtgaa acgaataatt
    25201 tcgatcatgc agatcgaatg tgtttggcga gttgttgcaa ctcgcctttc tgtatgtgtt
    25261 ttgaatgatg tttacagcgg ggtgctgccc gggttcagag ggcaaatcat aatgtagtca
    25321 gggtcgttaa caggtaacgc tccgggagat gggacacgtt cgcctttctg tgctgcaatg
    25381 gccagtgtac gcaacaaaac ctcactggcg cggtgaactg cttcctcttt cgttgtggca
    25441 gtgacgtgaa ggttatcaaa atcaccgatc tcaatacgat gcgctttctg cccattttct
    25501 tcatcgatgg tcgtgatatg ggcaggatac ggcacaaacc atgctgccac tttttgtgtc
    25561 tgccgccggc gggcatcaag cttactgacc agttgccatg cacgaagatg agtataccgt
    25621 ttcagcatat tcatggaacg atgtcctgat attgcagcaa tctccattac attcaggcta
    25681 cccagttcga agaagcggct tattgcttca tgccgtagat cgtgaaaatg caggtcctcg
    25741 atgcgaagtc gttgtgtggc tattctccag gcatttttaa agccggatgc ggtgtaatca
    25801 aaaacattgc cgtggagatt aacgggcatc atctgaagaa agttacgggc acgtctggac
    25861 agaggaacat cccgtgagtg accgtttttg gtttcaggta aatgagccac accgtggcgc
    25921 aaatcaatgt gttcccaacg taaggccagt atttcgccct gccgcatggc tgtttcaagg
    25981 gcaagatgga aaatgacata caacatcaga tttttttcgc ggaaatagcg agaaaggcga
    26041 cgttcttctg aagacgttag ccgacgatct cgtccggagg atactttcgg cttgcgaacc
    26101 agttcaaccg ggttagtacg acaggttccc cattcaacac gagcaatatt gaacagagat
    26161 gacagaaggg cgagttcaag acgtacagta ttacctgtaa tggctttacc cgttcggggg
    26221 ttgatttctg ctaaacgaac gtctctgtat gtagcaatat caacggttgt tatttcgtcc
    26281 atattccgaa gggcaatggg atatcgcttg ataacattgc tccggtaaaa ctcctgttga
    26341 tgccctttct tgtgaacaga aactgttttc aggtacttat ccagtgcgcg tgacagggac
    26401 attttacgga tgcgtggaga cggcatgcta acctcaaaag aggaaaaaat aatgccaaca
    26461 atcattctct gcaagcgtgc caatccggta cctggaaatc atcatcagcg tcgatatgga
    26521 cgaatataaa aacatttacg ttatatccca aaaacacaca ggtcttaggg aggtttaaac
    26581 tgtgtattaa cacctatcgt attgacgggc gtgaaatggc agaaacagaa gtcgttccta
    26641 ttgacatgcc tgactctaac ggcgagatga cttggcaagc caaaaactac acacagtatt
    26701 cgtcctactt tatgaagatt acttgcttga aatagcttat tggcaaatgg catatacagt
    26761 tattgaacca ttctgatctt cggttgtatc gcacgcagcc gaccatgagt tattggctga
    26821 tgggaatgac ctggtaagcc atatgtaacc agttcgtgag gtgcatattc cgccgccacc
    26881 tgtaacacgt tttccagttg gacaggtagc tgttgctcca ggaaagcgcc aacctgtcgc
    26941 ctgtgctgta gcaatttgaa gctcaccaga tcctaccttt
//
LOCUS       DAEVZA010000061        26704 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEVZA010000061 DAEVZA010000000
VERSION     DAEVZA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.061"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..207
                     /locus_tag="LAF92_002580"
     CDS             <1..207
                     /locus_tag="LAF92_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HBI9791569.1"
                     /translation="DLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRL
                     GDFRRQLIGQVKETLQKGKTPSEK"
     gene            355..1896
                     /gene="tyrR"
                     /locus_tag="LAF92_002581"
     CDS             355..1896
                     /gene="tyrR"
                     /locus_tag="LAF92_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBI9791570.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(1940..2446)
                     /gene="tpx"
                     /locus_tag="LAF92_002582"
     CDS             complement(1940..2446)
                     /gene="tpx"
                     /locus_tag="LAF92_002582"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBI9791571.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            2565..3530
                     /gene="ycjG"
                     /locus_tag="LAF92_002583"
     CDS             2565..3530
                     /gene="ycjG"
                     /locus_tag="LAF92_002583"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBI9791572.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(3505..4233)
                     /gene="mpaA"
                     /locus_tag="LAF92_002584"
     CDS             complement(3505..4233)
                     /gene="mpaA"
                     /locus_tag="LAF92_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBI9791573.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4543..5181)
                     /locus_tag="LAF92_002585"
     CDS             complement(4543..5181)
                     /locus_tag="LAF92_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI9791574.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVSALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            complement(5199..6131)
                     /locus_tag="LAF92_002586"
     CDS             complement(5199..6131)
                     /locus_tag="LAF92_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI9791575.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            complement(6131..7051)
                     /locus_tag="LAF92_002587"
     CDS             complement(6131..7051)
                     /locus_tag="LAF92_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI9791576.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            7189..8088
                     /gene="pgrR"
                     /locus_tag="LAF92_002588"
     CDS             7189..8088
                     /gene="pgrR"
                     /locus_tag="LAF92_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBI9791577.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            8425..10038
                     /gene="mppA"
                     /locus_tag="LAF92_002589"
     CDS             8425..10038
                     /gene="mppA"
                     /locus_tag="LAF92_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBI9791578.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(10089..11120)
                     /gene="ynaI"
                     /locus_tag="LAF92_002590"
     CDS             complement(10089..11120)
                     /gene="ynaI"
                     /locus_tag="LAF92_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBI9791579.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            11364..11621
                     /locus_tag="LAF92_002591"
     CDS             11364..11621
                     /locus_tag="LAF92_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBI9791580.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(11671..12621)
                     /gene="uspE"
                     /locus_tag="LAF92_002592"
     CDS             complement(11671..12621)
                     /gene="uspE"
                     /locus_tag="LAF92_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBI9791581.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(12773..13525)
                     /gene="fnr"
                     /locus_tag="LAF92_002593"
     CDS             complement(12773..13525)
                     /gene="fnr"
                     /locus_tag="LAF92_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBI9791582.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(13720..14235)
                     /gene="ogt"
                     /locus_tag="LAF92_002594"
     CDS             complement(13720..14235)
                     /gene="ogt"
                     /locus_tag="LAF92_002594"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBI9791583.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(14246..15772)
                     /gene="abgT"
                     /locus_tag="LAF92_002595"
     CDS             complement(14246..15772)
                     /gene="abgT"
                     /locus_tag="LAF92_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HBI9791584.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(15809..17254)
                     /gene="abgB"
                     /locus_tag="LAF92_002596"
     CDS             complement(15809..17254)
                     /gene="abgB"
                     /locus_tag="LAF92_002596"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HBI9791585.1"
                     /translation="MQEIYRFIDDTIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(17254..18564)
                     /gene="abgA"
                     /locus_tag="LAF92_002597"
     CDS             complement(17254..18564)
                     /gene="abgA"
                     /locus_tag="LAF92_002597"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HBI9791586.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            18740..19648
                     /locus_tag="LAF92_002598"
     CDS             18740..19648
                     /locus_tag="LAF92_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9791587.1"
                     /translation="MTFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            19978..20541
                     /gene="smrA"
                     /locus_tag="LAF92_002599"
     CDS             19978..20541
                     /gene="smrA"
                     /locus_tag="LAF92_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBI9791588.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(20562..21794)
                     /gene="dgcM"
                     /locus_tag="LAF92_002600"
     CDS             complement(20562..21794)
                     /gene="dgcM"
                     /locus_tag="LAF92_002600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBI9791589.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            22049..23032
                     /gene="zntB"
                     /locus_tag="LAF92_002601"
     CDS             22049..23032
                     /gene="zntB"
                     /locus_tag="LAF92_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBI9791590.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            23510..24883
                     /gene="dbpA"
                     /locus_tag="LAF92_002602"
     CDS             23510..24883
                     /gene="dbpA"
                     /locus_tag="LAF92_002602"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBI9791591.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(25012..25947)
                     /gene="ttcA"
                     /locus_tag="LAF92_002603"
     CDS             complement(25012..25947)
                     /gene="ttcA"
                     /locus_tag="LAF92_002603"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBI9791592.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(25999..>26704)
                     /locus_tag="LAF92_002604"
     CDS             complement(25999..>26704)
                     /locus_tag="LAF92_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI9791593.1"
                     /translation="SGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQSQNL
                     WKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQP
                     ALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVSSLG
                     ARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWI
                     DDMNDEQIAMLNARLS"
BASE COUNT         6731 a         6413 c         6632 g         6928 t
ORIGIN      
        1 gatctcgctg ctcgtttgtc tacccgcgca gctcagggaa ttggtgctgg acttctgacg
       61 gcacgactcg ggattaaagc tatggagctt tgccgcccgc tgccgtggat tgacgatgac
      121 aaacctcgcc tcggggattt ccgtcgtcag cttatcggtc aggtgaaaga aacgctgcaa
      181 aaaggcaaaa cgcccagcga aaaataatgc aatatcgggt gctgaccgga tatctttacg
      241 ccgaagtgcc cgtttttccg tctttgtgtc aatgattgtt gacagaaacc ttcctgctat
      301 ccaaatagtg tcatatcatc atattaattg ttcttttttc aggtgaaggt tcccatgcgt
      361 ctggaagtct tttgtgaaga ccgactcggt ctgacccgcg aattactcga tctactcgtg
      421 ctaagaggca ttgatttacg cggtattgag attgatccca ttgggcgaat ctacctcaat
      481 tttgctgaac tggagtttga gagtttcagc agtctgatgg ccgaaatacg ccgtattgca
      541 ggtgttaccg atgtgcgtac tgtcccgtgg atgccttccg aacgtgagca tctggcgttg
      601 agcgcgttac tggaggcgtt gcccgaacct gtgctctctg tcgatatgaa aagcaaagtg
      661 gatatggcga acccggcgag ctgtcagctt tttgcgcaaa aattggatcg cctgcgcaac
      721 cataccgccg cacaattgat taacggcttt aattttttac gttggctgga aagcgaaccg
      781 caagattccc ataacgagca tgtcgttatt aatgggcaga atttcctgat ggagattacg
      841 cctgtttatc ttcaggatga aaatgatcaa cacgtcctga ccggtgcggt ggtgatgttg
      901 cgatcaacga ttcgtatggg ccgccagttg caaaatgtcg ccgcccagga cgtcagcgcc
      961 ttcagtcaaa ttgtcgccgt cagcccgaaa atgaagcatg ttgtcgaaca ggcgcagaaa
     1021 ctggcgatgc taagcgcgcc gctgctgatt acgggtgaca caggtacagg taaagatctc
     1081 tttgcctacg cctgccatca ggcaagcccc agagcgggca aaccttacct ggcgctgaac
     1141 tgtgcgtcta taccggaaga tgcggtcgag agtgaactgt ttggtcatgc tccggaaggg
     1201 aagaaaggat tctttgagca ggcgaacggt ggttcggtgc tgttggatga aataggggaa
     1261 atgtcaccac ggatgcaggc gaaattactg cgtttcctta atgatggcac tttccgtcgg
     1321 gttggcgaag accatgaggt gcatgtcgat gtgcgggtga tttgcgctac gcagaagaat
     1381 ctggtcgaac tggtgcaaaa aggcatgttc cgtgaagatc tctattatcg tctgaacgtg
     1441 ttgacgctca atctgccgcc gctacgtgac tgtccgcagg acatcatgcc gttaactgag
     1501 ctgttcgtcg cccgctttgc cgacgagcag ggcgtgccgc gtccgaaact ggccgctgac
     1561 ctgaatactg tacttacgcg ttatgcgtgg ccgggaaatg tgcggcagtt aaagaacgct
     1621 atctatcgcg cactgacaca actggacggt tatgagctgc gtccacagga tattttgttg
     1681 ccagattatg acgccgcaac ggtagccgtg ggcgaagatg cgatggaagg ttcgctggac
     1741 gaaatcacca gccgttttga acgctcggta ttaacccagc tttatcgcaa ttatcccagc
     1801 acgcgcaaac tggcaaaacg tctcggcgtt tcacataccg cgattgccaa taagttgcgg
     1861 gaatatggtc tgagtcagaa gaagaacgaa gagtaagcgc gaatatgcct gatggtgcaa
     1921 caccatcagg catattaaat tatgctttca gtacagccag agctgcttcg taatccggct
     1981 cggtggtgat ttcatccacc agctggctga aaatcacatt gtcattttcg tcaataacca
     2041 caacggcacg tgctgccaga cctttcagtg ggccatcagc aattgccaca ccgtaggctt
     2101 gcaggaattc agcgttacgg aaagtggaga gggtgataac gttgttcaga ccttctgcgc
     2161 cgcagaaacg agactgggcg aacggcagat cggcagaaat acacagcaca acggtgttgt
     2221 cgatctcggt tgccagttgg ttaaacttac gtactgatgc ggcgcaaact ccggtatcaa
     2281 tactcgggaa aatgttcagc actttgcgtt tacccgcaaa ctgaccgagg gtgacgtcag
     2341 acagatcttt tgccacgaga gtaaaagtct gcgctttgct acccgcctgc gggatggaat
     2401 tggcgactgt aaccgggttg ccctggaaat gaacggtttg tgacatgatt atctttcctg
     2461 tttacatata gttaacgtca cacctagttt atgctaactg tcaataacac agcaaacgct
     2521 atttgcgctt aatccgcaga ccaccgcgac aacaaggagt aaagatgaga accgttaagg
     2581 tatttgagga agcctggccc ttacataccc cgtttgtgat tgcccgggga agtcgcagtg
     2641 aagcgcgcgt ggtggtggtt gaactggaag aagagggtat taaaggcacc ggcgaatgca
     2701 cgccgtatcc gcgttatggg gaaagtgatg cctcggtaat ggcgcaaatt atgagcgtcg
     2761 taccgcaatt agagaaaggg ctgacacggg aggagttgca aaaaattctc cctgccggcg
     2821 cagcacgtaa tgcgctggat tgtgcattgt gggatctggc ggcgcgaaaa cagcagcaat
     2881 cgctggctga tttgatcggc ataacgcttc ctgggacagt tatcactgca cagacggttg
     2941 tcatcggtac gcctgatcag atggccaata gtgcatcaac actctggcag gcaggcgcga
     3001 aattactgaa agtgaagctg gataaccatc ttatcagtga gcggatggtg gcaattcgca
     3061 cagctgtgcc cgatgcgacg ctgatcgttg acgcaaatga atcctggcgt gcagaagggt
     3121 tggcggcgcg ttgccagcta ttggcggatt taggcgttgc gatgcttgaa caaccgcttc
     3181 ctgcgcagga cgatgcggcg ctggagaatt ttattcatcc gttaccgatt tgtgcagatg
     3241 aaagttgtca tacacgtagc aatctgaagg cgctgaaagg gcgctatgag atggttaaca
     3301 ttaagcttga taaaaccggt ggtctgacgg aagcgctggc gctggcgact gaagcgcgtg
     3361 cacaaggttt cagtctgatg ctgggctgca tgttgtgtac ctctcgtgcc attagcgccg
     3421 ctttaccgct ggtgccgcag gtcagtttcg ccgatcttga cggaccgacc tggctggcgg
     3481 tagatgtgga accggcgctt cagttcacga cgggcgaatt gcatctttag gatgccagcg
     3541 cagcaagttt gccatcgcaa agagatattt ttcgctggct tcgtcggagg agatgggcgg
     3601 aaactctgcg gtgatgcaat gcaagttgag atctgcacac caactgccga aagagccagg
     3661 cgtttcataa ccgacgctgg ttaccagagg caattcaaac gcctgggcca gccattcgcc
     3721 taattcgctg tgtctgggat cttcaataca ggccagtgga tcgtggaatg agaccaccca
     3781 ggcaggttga atgcggtgta taagctggca cagcgcctgg gtttcaggtt ctgagccagg
     3841 tttgtcacct gtcagtaata cgacatcacg ttcttcagcg gcgctgttcc agcgataaac
     3901 cgtttcacct tccttccagt tcgccgccgg aaagtttctg tttaaatcca caccattagc
     3961 attggcccgt aaccccagct ggcagccgtc aggattcaca cacagcacca catgatggcg
     4021 gcgcaaagaa ggtgtcaaag tccgcagagc acaggagagc gtgacgaccg aagagttttc
     4081 atcaccgtga gtgccagcca gaatcaaacc actttcgcga ctggctgcag tggccggaaa
     4141 ccagattaac ggcgcgccca ataatgaacg tccgtaatgt tctgttccgg gcggaaatgc
     4201 gccgcgttcg gcgcgtgggc ggtttacggt catgatcgtc tctggttcag gaagtattac
     4261 aggcagtgtt gtgcaaattt tgcggattat caaatccttt tcttcaacgc tatgtcatcg
     4321 atcttgtatc actttttttg ccggaagttg tttgcatttt tattgatgtg cagatattag
     4381 aattatgctg acttagatgt aagtcgtaat gttatataca tggcgtattt tattcttact
     4441 ggttctacac atgttaatcg catcggattg atcagttcac cttaataagt ttaataaatc
     4501 gtactgcaca tagtacatta cgatggtaac tatgtaattg atttacatga ataatggctt
     4561 gtcaccatca gtcacgggtt gattgatacc tatattctca ccaatgtgtt tctcaggttt
     4621 atcgatgata tctgtgatta atgctgagac actcttacgt gatacgatcg tacctttgaa
     4681 tggttcattg cgtgaggtaa gctcataatc aataatatct tcatcagtca gccaggcagg
     4741 gcgaagaatt gtatattcca gtccagaagc ctcaattgca tccgcagccc tgcggaatgg
     4801 ttttaggggc tcaccaataa ctgcattatt ccattcgcca aattttcctg gaacttcatc
     4861 ataaataccg agggaaagaa caaagatcag acgtttaaca tcacaggctt tcatcgcagc
     4921 gatcactgaa tttgcctgaa tatctaaatc ttcccccgta aggttggcat acacaacgtc
     4981 ctgtccctgc atggcctgct tcagtgctgc atgattcagt acatctccca taataatctt
     5041 gctattagtt ggatagggtt tgtggatttt tgctggctga cgggcaaata aagtttgttt
     5101 aatggtttgt ttatctgcca gttgattaat cacatggcgg gctatctgac cgccagcacc
     5161 aagaatcagt acatttttca taatatttct catgaaactt atcccagatg ggtgttgaaa
     5221 aaaggaataa ctttttccag cgcctcctga actgcttccg gtttgtcata cagttcataa
     5281 tgtgagaaat cgggtaatga aactaactga cgatcctgcg aaaccatcgc ccggccatag
     5341 acttccagtc catcacgata agcaccgaag gcaccgactt tttcaccaac tactaccatc
     5401 accggctgag tcagtaacac ttcagtaaat gcgaaggcat cccaggccag cgttttctgt
     5461 gcatgtgaga acaacatttt tgtcgcacct cccggttgtt gcccgcgtgg tgttttatag
     5521 tagtcagttg cttcataaac atcgcgttcg gtcagtccat gggcttttgc tgcatccagg
     5581 ctggcgggaa gtaattcgtt gatctgtaat tctccaccac gagcctcttt ggttcgttgc
     5641 gacgccattg cattcaatgc gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga
     5701 cgcccgatat taacacccgt aatactcact actgctttaa ttcgtttttc tgtcagcgcg
     5761 gcattaatgg catagccacc acccccacac actcccagca cgccaatacg ttcagcatta
     5821 acatatggca gagttactgc ataatcgata acacggctaa tatcctctac gcgctgtgtc
     5881 ggatcttcta tccagcgagg ctccccacca gattctccct ggaaactggc gtcataagcg
     5941 agaacgagat agcctttttc agccaatgct ttgccataga tattgccaga cgtctgctcc
     6001 ttacaactgc caaaaggatg gacgctgata atcgccggat actgttttga ctcatcaaaa
     6061 tttggtggaa actgaatatt ggcagcaata tgccaggcca tatcactatt gcggatttga
     6121 atatgtttca ttacagagtt tcctcaaaga acggagctaa tactgaaatt gcttcagcca
     6181 cataggcttt gccgtcataa agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt
     6241 tatcctggct tgatgcccga tcgtacaggt cgtcactcat ccatttgctc cctgcctggc
     6301 tgcccgccac aatctgcatc ggctgagtca ggtacacttc cgccatatgg taagcatcat
     6361 aggtaataat ctggttaagg ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg
     6421 cgcgaggggt gtggtaatac tcccaggcct gacgcagttc ttcgttcggc gcatcggact
     6481 ccttcattgg tgccagtggc ataatggcgt attctccgct gctaatatcg ctggttctgg
     6541 cgtttgaacc cgcttcaacg tatggcaggg catcaataga tttcacattg ttttcccaac
     6601 cattacggaa catcgaacca atattgaccg cactaacggt accgatggcc ttgatgcggc
     6661 gatcctgaat tgcagcattg gctgtatatc ctgcaccggc acaaattccc atcgcaccaa
     6721 ttcgggtatt gtcgacataa gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt
     6781 cagtacgaat gtatgggttt tctaactgac gcggctcgcc gccactttca ccttgataag
     6841 atgcgtcata agcaatagtg acaaatccct tttccgccag ttttttggca taggttccgg
     6901 ccgtttgttc tttaacgccc ccacctgggt gagataccac aattgcctga tactgacggg
     6961 tttcatcaaa ttttggaggg aaatagatca ctgcagacaa agagatggtt ggattattgc
     7021 tgttagtgaa gctgacttta ttattcatca ttccgttcct catggagttg tcggttcgtt
     7081 ttaacggttg gtgatatcac tatagatatt gatcattaag ttgattagac ccaaaatcat
     7141 gattagacct atcatttaaa tgattaatag attgcatgga gatactgaat gaagcgtgaa
     7201 gaaatcgctg atctgatggc gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca
     7261 gcagcccgcc tgagcatggc gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa
     7321 cgattgggat tgcggcttct gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc
     7381 gagcatcttt tgtctgttct tggccctatg ttgcatgaca tagattcagc cctggcatcc
     7441 ctgagcgagc tgcagaaccg cccatccggg acaatacgta ttactactgt agaacatgca
     7501 gcaaaaacga tattgttacc agcaatgcgc acattcctga aatcgcatcc tgaaattgat
     7561 attcagctca ccattgatta tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc
     7621 gtccgtctgg gtggggagat ggataaagat atgatcgcca ttcgaatcgg gccagatata
     7681 ccaatggcta ttgttggctc accggattat ttttctcgcc gaagtgttcc aacgtcagtg
     7741 tcacaattaa tagatcatca ggcaattaat ttgtatcttc ccacatcggg tacagcaaat
     7801 cgctggagat taatacgcgg tggacgtgaa gttcgtgttc gcatggaagg tcagctttta
     7861 ctgaatacga tagacctgat cattgatgct gcaattgatg ggcatggatt ggcgtatcta
     7921 ccttatgatc aggttgagcg ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa
     7981 ttcacaccag atttacccgg ttatcacctg tactatccac accgtcgaca tgctggctcg
     8041 gcattctcat tatttataga taggctgaag tataaaggtg ctgtttagca ctacttgctg
     8101 atacattaat ttaatttctc tcttaacgta ttctcagttc ctttcaacgt tttggttatt
     8161 ttttattctt cgtacaatgg cgacagatgc tgattatgat accgaaaact ggtttgaacg
     8221 tgcgaagcct gaacgtagtg ttcggtgttc tatgtgcttt accgcatttt ggcgtcgatt
     8281 atttacacta aatctgattt aatatattga tacataaaac attttatgct tcctgtgtca
     8341 cttttttgat ggaagttgtt tgcatttctt taaggcgaaa caaataatta cgcatcaatt
     8401 ttaatgtcgg ttagagggaa acttatgaag cactctgttt cagtcacgtg ttgtgcgctg
     8461 ttggtcagca gcatttctct ttcgtatgct gcagaagttc cgagcggcac agtactggca
     8521 gagaagcagg agctggtgcg ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa
     8581 gccgtgggcc tgccagagat tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag
     8641 aacgagaaag gggagattgt ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt
     8701 atctggactt ttaccctgcg cgataacgca aaatgggcgg atggcacacc ggtaacggcg
     8761 caagattttg tctacagctg gcaacgtctg gtggacccga aaactctatc gccatttgca
     8821 tggtttgccg cgctggcggg aatcaacaac gcacaggcga ttattgatgg taaagctacg
     8881 cctgaccagc ttggcgtcac cgcagttgat gcccatactt tgaaaattca gcttgataaa
     8941 ccgttgccgt ggtttgtgaa tttaaccgct aactttgcct tcttcccggt gcaaaaagcc
     9001 aacgtagaaa gcggtaaaga gtggacgaaa cccggaaatc tgattggcaa tggcgcttat
     9061 gttcttaaag agcgcgtagt caatgaaaaa ctggtcgtgg taccgaatac ccattattgg
     9121 gataacgcca aaacggtact gcaaaaagtg accttcctgc caattaatca ggaatccgca
     9181 gccactaagc gttacctcgc gggggatatt gatatcaccg aatccttccc gaaaaatatg
     9241 tatcagaagc tgttgaagga tattccgggg caggtttata cgccgccgca gctcgggacc
     9301 tattattatg cgtttaacac gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca
     9361 ttaagtatga cgatagatcg ccgcctgatg accgaaaaag tattagggac gggcgaaaag
     9421 ccagcgtggc attttacacc agatgttacc gccggattta cgccggaacc ttcgccgttt
     9481 gaacaaatga gtcaggaaga actgaatgcg caggcaaaaa ctttgttgag cgcagctggt
     9541 tatggtccgc aaaaaccgct gaagctgacg cttttgtata acacttcaga aaaccatcaa
     9601 aaaattgcga ttgctgtagc atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg
     9661 caaaatcagg aatggaaaac ctatatcgat agccgtaaca ccggcaattt tgatgttatc
     9721 cgcgcctcat gggtggggga ttataatgaa ccctccactt tcctgacatt attaacgtca
     9781 acgcattcag gaaatatttc acgctttaac aatccggcat atgacaaagt tctggcccag
     9841 gcatcgacgg aaaataccgt taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc
     9901 ctcatggagc aagcaccgat tgcaccaatt tatcaatata ccaatggacg attaatcaaa
     9961 ccgtggctga aaggttatcc cattaataac cctgaagatg tggcgtacag tcggactatg
    10021 tatattgtga agcattgatg tgatgggaac tggcgttacc cttgtggata acgccagtga
    10081 tgtctgattt agcgaccctg ttccggcggt gtaatgttat ccatatacag cgtctggctg
    10141 gggaaggcaa agtccgcgcc gtgtgactgt acaatatcga taatcttcaa ataaacgtct
    10201 tgctgtgcag caagccattc agcccatacc gtggttttgg taaagcaata aaccataata
    10261 ttcaatgacg agtcggcaaa ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg
    10321 gccgggtgat ttttcagcat ctcacgtacg gcttcgacaa taacgcccac ttttgccgca
    10381 tcctcataac gtaaaccaat ggtcgtggta atgcggcggt tggtcattcg tcctgggttt
    10441 tctacgctga tcgacgaaaa cagcgagttc ggtacgtaca atggacgatt atcaaaggtc
    10501 gtaattttgg taattcgcca gccaatttcc gctactgtac cttcgatatt tctgtccggt
    10561 gaacggatcc agtcgccaat actgaaagga cggtcgaaat agagcatgat cccggaaaag
    10621 aaattactca gaatatcttt accggccata ccgacagcca gaccaccaat accaccaaag
    10681 gtcaacaagc cagaaaggct catgccgaaa tgttcgccat aaagcagaac aagcaccaca
    10741 ataatggtga ttttgataat gcgcgacata atccgcgcac tggtgatatc gcgacctttt
    10801 ttaatctgct gtttttcaaa ctgattaatc agcaaaaata gcttaatcgt cagaataacc
    10861 gcaatcaggg acgtacagat aaaatcgata acgcctgggg tgataaattt gagtttatag
    10921 ttttctataa cataattaat aatgctacca acagcactga taattatggt gtagattaaa
    10981 aattgcaccg catggaataa aaaacctttt cttttacgat ttccgcggcg aaaccaaaag
    11041 ctcatcagaa tcaatgctgc gcagctaccg aaaataatga ccagattaag cgcattattt
    11101 gtaaacagtt cagcgatcat tgttttatca ggctcctcca gataattgtc gtcatgccgg
    11161 aaacccctgg cggggctatt ttaccgcgac aattcattca gatcatcaat agtcagggaa
    11221 ggaagtagca atattagcta actaatcaat gcgttgattg taaatccagc taagaggtga
    11281 ggttttcaga gcagacaacg gtgaaatgtc atggtattgt tacgtttagg taacaagaaa
    11341 tttgtctgca caaggattac atcatgatta tggcgaaact gaagtcagcg aaagggaaga
    11401 aatttctctt tggtttgttg gcggttttca ttattgcggc gtcggttgtg actcgcgcga
    11461 ctatcggcgg cgttatagaa cagtacaata ttccgctgtc tgagtggacg acatcaatgt
    11521 atgtgattca gtcatcgatg atttttgttt atagcctggt ctttactgtg ttgctggcaa
    11581 tcccgttggg aatttatttc cttggcggcg aagagcagta agtaaaaaat aggcccgata
    11641 actcgggcct tgtcagttat tgaagagtcg ttaatcgtct tcttcgtcat ccagttcaac
    11701 gggtgtctga tactggtcag gtttaatgac caacagatcg cagcgaagat gatcaatcac
    11761 ctgttccgcc gtgttgccga ggaatgctgc tgaaataccg gtgcgtccta ccgtgcccag
    11821 aaccacaatc cccgcctgta agtgctccgc caaatcagga atcacctctt ctggcagacc
    11881 tttttctacg tgcgtcatgt tttcattaat gccgaatttc tgccgcagag ctttcattgc
    11941 cagcaaatgt tgcccacgaa tggcatcgtt ataaacgctc ggatcaaatt ccggcagttc
    12001 aatcgcgata ttaattggcg ttaccggata agcgccaacc agatgaactt cggtatggtt
    12061 gacttgttct gccagttcga tcgtctcttt gaccagtttt tcattgagcg cattatgata
    12121 cggctcttca ctggcgagat tcaccgccac cagcgccttg cctccttccg gccacggctg
    12181 gtctttcacc atccacaccg ggcttgggca tttgcgtaac agatgccagt ccgttggcgt
    12241 aaaaatcacc gcttccagac ggtcatgttg gtgcgccatt tttagcacca aatcgtgccc
    12301 gccgctgatc acttcctgaa tgatggcttc gaagggacgg ttatgccaga ccactttaat
    12361 ttcaatggga acgccagcat tgagataata ttttgcctgc tcgtggatcc aggctgtacg
    12421 ctggctgatg acgccctgac gcatagcggt acgttcgtcc ggggagagca gggtggtcat
    12481 ttcgtatgag aagtcataga tcggcaaaaa ggctttaatt ttgccaccaa tccgttgatg
    12541 taaataaaca gctcgccgca atgctggttg gtcgtcctgg ttaggatcga taacaacgag
    12601 catgttctga tacatagcca tacagggtct ccttacaaca actgtcaacg cagtttgtaa
    12661 ttaaaagatt aacccatatc tggtgaatga aacagtgatg aaccttctgc cagatcaata
    12721 aatcagaaaa atttaatgat atgacagaag gatagtgagt tatgcggaaa aatcaggcaa
    12781 cgttacgcgt atgaccagca agctgggcca gcgcatcgtt attttcgatg gtgatgtatt
    12841 tacctttgac tgccagcatg ccgcttttct ggaagcgacc cagcagacgg ctgatggttt
    12901 ctaccgtcag gcccagatag ttaccgatat cgccacgagt catcgtcagg cggaattcac
    12961 gaggggagaa gccgcgttgg gcaaaacgac gggacaggtt gtagatgaat gcagccagac
    13021 gttcctcggc atttttcttc gacaacagca ggatcatgtc ctgatcgcct ttgatttcac
    13081 cgctcatcag acgcatcatc tgctgacgca gattcggcat tttaccagac aaatcgtcca
    13141 gcgtttcgaa cgggatttca cataccatcg aggtttccag cgcctgcgcg aagctcgggt
    13201 gatggccgct gccgatggcg tcaaatccca ccaggtcgcc tgctaaatgg aaaccagtga
    13261 tttgctcgtc gccttgctca gtgatggtat aacttttaat cgtaccggag cggatggcat
    13321 aaagcgattt aagttcatca ccagccttaa acagcgtctg gcctttctga ataggcttct
    13381 tccgctcaat gatattatca agctgatcaa gctcatgttc gttgagtgtg aacgggatgc
    13441 aaagctggct gatgctgcaa tcctggcaat ggatagcaca accgccagac tgaatgcgcc
    13501 gtataattcg cttttccggg atcataggtc tgctcaagcc gtaattgata tttgtcaatt
    13561 ttaacatctt tttagggagc aagtaagtct aagcaaacct taacagcaga gaattccgat
    13621 attagatgta aatatatgtc tatctatttg aaaaccctta agttgttaag ggtaacttta
    13681 cataaaagtg tgaacaagct ggcacaaatt gtttaatgtt tacagcaaaa gataaccttc
    13741 gtggcgcaat aaccactctt ttcgctgaac tccgcctgca tatccggtca tggtgccgtt
    13801 tcggccaata acccgatggc aaggtacgac gatgctgatg ggattcgatc cgtttgccgc
    13861 accaacggca cgcgccgcgc cagggcggcc caattgttca gccagttggc cgtaatgcat
    13921 tacctgcccg caggggatag tgcgtagagt tttccagact tcgcgctgaa atggcgtccc
    13981 ccctgtggca gtgggaagcg tatcaataat gctaagatta ccggcaaaat aatcactaag
    14041 cttgtcgctt aaaccgcctg ggttggtggc agaaatgcgc tcatagcctt ctttgcgata
    14101 atggatgtcc agcagctgca ccatgcgttc gctgtactct tcccattcaa ccgcccgcag
    14161 gcgaaattgc tcatcgcaaa tcacccacag tggacccagt ggcgtggcaa ttttttcttc
    14221 aagtaatctc agcatccgtt ctctcttaag acaaacgtgg gtaaatcccc ggacctatcg
    14281 gcagaccaac aagataccac gccagcaaca tcagcagcca taccaccaaa aagataagcg
    14341 gatagggcaa gactaacgaa tagtaagtac ccagtttcgc gtctggtttg tagcgttgca
    14401 ggaatccaag aaacagtggg acaaaaggag ataccggcgc taaaggcaat acggatgagt
    14461 cggcaatacg aaagaggatt tgcgcaaatg ccgggtgaaa gccaagtagc ataaacattg
    14521 gtacgaaaat gggggccaga atcgaccaga ttgcggaacc gctggcaata aacatgcata
    14581 agaaagagga aagcaacgcc agaccgacaa acgccggtat gccgctaagc cctgaacttt
    14641 ccagtatatc ggtcagcccc acggcgatga atttccccat gttgctccag ttaaacatgg
    14701 cgacaaattg ggcgagggga aaaaccatca cgataaatcc cgccatctct ttcatcggtt
    14761 caatcattaa atgcggtaaa tccgcctgac gtcgaattgt gcgggtagcg atgccataag
    14821 ccagcgagac aacaaagaaa aaaagaatga tcagtggcac gataccttta ataaagggtg
    14881 atggcatcac ggtgtgatta atcggatcgc gcaatatccc gttttccggg atcaccatca
    14941 gcgcgatcgc agcaataaaa agtagcgata cgacacctgc tatgcgtaaa ccaaaacgct
    15001 gactttcggt caatgtctgc agtttctcat cgctgtttcc ctgccattga cctaatcgtg
    15061 gctcgatgat tttgtcggtt atcaggccgc caacaatcgt cagtacgact acggagctgg
    15121 ccataaaata ccagttatca attacactga cgtgcatttg cggattgaac gcagccgccg
    15181 cttccgtgct gatccccgac agcaacacgt cggttgtgac aatcagtaaa ttagccgtaa
    15241 agccgcaacc tacgcctgca atggcagcca gtaaacctgc aactggatgc ctgcccaccg
    15301 ccagaaaaat cagcgcaccc atcggtggca tgatcactaa cgccgcatcg gaagaaatgt
    15361 ggctgaaaaa agcaataaac agcaccatat aactggcgta gcgggcatta acatgcgatg
    15421 ccattttaac cattagcgct ggcagtaagc cgacgcgctc cgccagaccg gcacctaaaa
    15481 ccagcgccag gatcgctcca agtggagcaa aaccgctaaa gtttttaatc acattgggta
    15541 aaaaccagtg taatccttcc acactgagca ggtttttcac caccaccggc gtaccatcgg
    15601 tcgggttttt cgcactgacg ccaaaggctg acaaaattgc cgtcgtcacc atgagtacga
    15661 ttatcaaata gataaagagc aaaaaaggat ggggaacctt gttaccaatt ctttcgaccc
    15721 agccatagag cttcccggat tgggaggacg acggtatgga tgacatactc atgggcattc
    15781 ctcggttgtt gtgtttgcgt tgttgttgtt attttaaagg tgacggtgtc acgtttttcg
    15841 ggatagggca gtgatacggt tgcgtgtccg taacttgctg atgctcttgt tggcattctt
    15901 gcaatagccc tgaatcaatg aagagattga ctgtggttgc tgccatagtt ttcgccgcca
    15961 gcagcattcc tttatgagca atagatgttc gcccctggct aaccagttgc cacgtatgta
    16021 gcggtgtacc gacggcaaaa caggggctga aacactgggc aacaggcagt ttccaactga
    16081 cgtcgccgac atcagtcgat gccgcaagca cgttatcggt ggcggcatat ggagcgacct
    16141 cattcgccag taccgtttca cgatgacgta gtgcaaaaac cttgccgttt tcgccaccgg
    16201 ttgcggcgat attattcaga ctgttttgcc gatcgttggg ggtgagcgta gcctgaattt
    16261 gtttcgcaaa agccagttct tcggagttcc attccggggt accaaaatgg gatagggcat
    16321 ggtacatggc attttctaag gtgcgattcg ggagataact ggaacaggct ttgtcgaagc
    16381 ggcattcaac cgtggtttcg gtcatcaatg ccgcaccttc ggcgattttg gcgacccgat
    16441 cataaatatg ctgcacgtcg gtcatttcgg gggcacggat aagataaagc acttctgcct
    16501 gcgcctggac cacgttgggc gagatcccgc cgctatctgt gatggcatag tgtacgcgcg
    16561 ctttttcaat aatatgttcg ttgaggaagt tggtgccagt ggtcatcaac gttacggcat
    16621 caagggcgct tcgtcccaaa tgaggggaat tcgcggcatg tgctgcgatc cctttaaagc
    16681 gccatgatgc ctgaatgttt gccagcgtgc gggtattgaa cataccggca aaggcttccg
    16741 ggtgccaggt gagtgccgca tccacatcat caaatacccc ctcgcggacc atgaacgttt
    16801 tacccgagcc gccttcttcg ccaggacaac cataaaagcg cactgtgccg ccttgcccat
    16861 attgttccag ccatttcttg acggctattg cagcggcaaa ggcggcggtt cccagcaaat
    16921 tgtgtccgca accgtgacca ttttcaccgg gcgtcacgga tgtaggttgc gcgcaacctg
    16981 cttgctgact taaacctgcc agggcgtcat attctcccag cagggcgata accggtttgc
    17041 cttgaccaaa cgaagcaata aaggcatttg ggatattgcc tacgttgcgg gtaacggtga
    17101 agcctgcaga ttccagcgcc gaagccagat gctccgctga ccagaactct tcaaaacgtg
    17161 tttctggatg atcccagatt tgatcggcaa tatcggtata acgttggcga tcggcttcaa
    17221 tcgtatcgtc gataaaacga tagatttcct gcatcagata cctcgcgtcc agggaaaatt
    17281 gagcgcggtg cgcgccagcg tttcgacggc aatagcgaga acctgctcgt caaaatcgaa
    17341 tttttcgttg tgatgacctg ccgccagctg tgtgccaaac accacgtagg aggcttgccc
    17401 ttgatgttgc tgcacgcggg ccatcattaa tgtggcatct tcggaacccg caggcgcttc
    17461 aacacgttca atggcctgat tgacccccgc gacctgagcc gcctgacttt gcaaccatgc
    17521 gacccattgc ggcgaaggag aactggcggt agctgcaccc atcagacgag tttcaacgcc
    17581 gacaccatac atggttgctg cgccctgaat cgcttgttgt gcacggtcaa aaacatattg
    17641 attaatgacg tcgctggccc cgcgtgtttc cactttcagc aacgccgagg caggaacaac
    17701 gttacggccg cttcctgcct gcataacgcc cacgtttact ctggaagctc cttcgctgtg
    17761 cggggcgatt gcatgcagtg caagagttgc ttgtgctgcc gccaacaagg cattgtgacc
    17821 gtcttctggt tttgcgcctg cgtgagcggc ggtcccggtg aagtgcgcgt caaatttggt
    17881 ggttgccata aaattatcac tgccgcacac cacggtgccc gcaggtacgc cagtgccaat
    17941 gtgcacggca gtaaaataat caacatcatc tacgacacct gcatcgacca tcgcccgcgc
    18001 gccacgcgta ccttcctctg caggctgaaa aatcagtttg atgacgccat gtagtccgga
    18061 ctcgaattgt ttaagggtat gcgccagccc aagcccaatg gcggtatgtc catcatgacc
    18121 acaggcatgc atcattccgg cgttacatga cgcaaaaccg tcgcggtagg ggcgatggct
    18181 gacatcctgc tcttcactga gatccagcgc gtccatatcg acacggaaag ccatcaccgg
    18241 accggggcga ccggtatcca gggtggcgac gataccagtg aaaccacctt caaaaaccgc
    18301 aatccattgt gctagcgcac cctgttgacg agcgcgctcg aattcgcgtt gtagagtgag
    18361 ttcatcaggt agtcccatcc ggctactttc attaactact tcgcggccca gcgccagtga
    18421 atagccgagc tgctgcaatt cttccgcaac aagggtggca gtgcggaatt ccacccagcc
    18481 agactctgca tagtgatgaa aatcacgtcg ccagtgcgat aattttgggg caagcgaatt
    18541 aacaaattga ttcaaagact ccataacctt tcccgtcatc agtaaaaagt gtgacccggt
    18601 tcacgtagcg atagttttta cttatcacta actgattttt cacagtttta accgttcata
    18661 aattaccctg acacaatcat ctgcattaaa gtagatgcca gtttctttgg tctgataaat
    18721 aacggttatc ggtggcgtca tgacttttca ggtaaaaatt catcaaattc gtgcttttgt
    18781 tgaagtggct cgtcagggca gcattcgcgg agcgagccga atgttgaata tgtcgcaacc
    18841 ggcactgagt aaatctattc aggagctgga agaaggatta gcggcgcaac tcttttttcg
    18901 ccgtagtaaa ggcgtgacgt taactgatgc cggtgaaagt ttttatcagc acgccagtct
    18961 tattcttgaa gagctgcgcg cagcgcaaga ggaaattcgc caacgacaag ggcaactggc
    19021 agggcagatt aatatcggca tgggggccag tatttcccgc agtctgatgc cagctgtcat
    19081 atctcgtttt catcagcagc atccgcaggt aaaagtacgc attatggaag ggcaactggt
    19141 gtcgatgatt aatgaattgc gtcagggaga attggatttc accatcaata cctattatca
    19201 gggaccgtac gaccacgaat ttacttttga gaaattactg gaaaagcaat tcgcgatctt
    19261 ttgccgcccg ggacaccccg ccattggtgc ccgttcgatc aaacagttac tggattacag
    19321 ctggacaatg ccgacgccac acggcagtta ctacaaacag ttgagtgaat tgcttgacga
    19381 tcaggcgcaa acgccacagg tcggtgtagt ctgcgagacg ttctcagcct gtatcagtct
    19441 ggtggcaaaa agcgattttc tcagcatact gcctgaagaa atgggctgcg atcccttgca
    19501 cggacagggg ctggtgatgt tgccggttag cgaaatttta ccgaaagcgg cctattattt
    19561 gattcagcgg cgtgatagtc gccagacacc actgaccgcg tcattaatca cgcaattccg
    19621 gcgagaatgc ggctatctgc aaagttaaaa ctgcataaaa aaatagagtc tgtcgacatc
    19681 cgccagactc tacagtacac acagcagtgc atccgcgtct taagtccggc attgtctcct
    19741 ctgcgccggt gactgtgtta taacggtttt aacctttagt tgccaatttt cttcagtgac
    19801 agatttcacg aaaatataag ttacattatt aatatcgtga atgaataatc atgcataagc
    19861 attttgctta aaatatcggc aatatttgga acttattact ggaattttgg gtaatacgtt
    19921 gttggaccga cccggtctgg ttatcatatc gcgctcttaa ttgcgggagg atgtaacatg
    19981 aaccttgacg acaaatcgct gtttcttgac gccatggaag atgtccagcc gctgaaacgt
    20041 gctaccgatg tccactggca tccaacgcgt aaccaacgtg cgccgcagcg tatcgacacg
    20101 ctgcagcttg ataatttcct caccaccgga tttctcgaca tcatcccact aagtcagccg
    20161 ctggagtttc ggcgggaagg gttgcaacat ggggtgctgg ataagctgcg cagtggtaaa
    20221 tatccgcaac aggcgagcct gaatcttttg cgccagccgg tggaagagtg ccgcaaaatg
    20281 atgttcagtt tcattcaaca agccatggct gatggtttgc gtaacgtgct gattattcat
    20341 ggcaaaggac gggatgataa atcgcatgcc aatattgtcc gcagctatgt ggcgcgctgg
    20401 ctgaccgaat ttgatgatgt tcaggcatat tgcactgcgc taccgcatca tggcggcagc
    20461 ggggcgtgtt acgtcgcact acgtaaaacg gcgcaggcga agcaagaaaa ctgggagcgc
    20521 cacgctaagc gcagtcgttg atctcgagac gcatccgcgg cttatgccgc cagcacgcgg
    20581 ttgcgtccat catttttcgc ccgatacaaa gcatcatcaa cgcgtttaaa cagttcatcg
    20641 atgctttcat ttccttcgtg atgcgccaca ccaatgctga cggtaaagcg tggtaagccc
    20701 gaaatactca cttttgccac gcttacgcgg atagtttcag ccagcgaaag cgcggtatcc
    20761 agtggggttc ttggtagcaa taagacaaac tcttcgcctc cccaacgaaa caccaaatcg
    20821 cctttgcgag cgcaactttc gagggtgcgg gcgagggcgc ataacacctc atcaccttta
    20881 gaatgcccat agagatcgtt aatgtgttta aaacgatcgg tgtcgatgag caacaagctg
    20941 taatcctgag cgatggcgag atgctgcatt tggcctggct cggtaatgtg ataaaactgt
    21001 cgccgattca gtaatccggt catcgcgtca tggtgagcag catgttccag ctgctcctcc
    21061 agccgttttt gctcagtaat atcatgcaca atacataaca tgagcttgtc gccataaatt
    21121 tcaatcggcc cggcataggt ctgcacatga cgagtagatc catccgccag tttatgaaca
    21181 aaattcaaag gtttatggcc gccgggtaaa tgcgaaattt catgcatgat aggcatgacg
    21241 cgacgcccga gcatattaat ttcccaggta tgtttctggc acatcgtttc atggttataa
    21301 ccatagaaat tgagcgcggc gaggttagca tcgacgattt ggccatctcg tgaggggtca
    21361 atcaacaaca ttggtgcaga gttagtcaga aaaaagcgcg cataaaaacc ttgttttttg
    21421 cgctgataat ttgccgagcg actggctttt aaacccagcg ttgccggcgc ttcgatacct
    21481 tcgaaaataa tcaccggttc tgtttctgtc agctctcgca aaacaagccg acagcttaat
    21541 gctgtttcct cttctttacg ctgaacagtg aggatttcga taatatcgtg ttggttttgc
    21601 agatcggaga ggtatttcgg cagttctttt tgtgaggaga cggaataggg tccggttcgt
    21661 agctgactaa acgtgaggtc ttgcatcaac agtcgcgccg cgctattggc ataaattaac
    21721 tgttcctcaa agggcgaaac aatccagaca ggactggtga gtaagtccag ggtattgaag
    21781 ttgtgcgtaa tcattgagat cccgttattt ttatcaattt ttgttgctat ccgatcgcaa
    21841 aaaagccacg tcatatgatc agataattct gataatgata gacgctattt aacacttcac
    21901 acggtttgta tacggaaaag cattttgctt tttgtattca atttagacag aattttatta
    21961 atcatttcag ggtaatgggg tgatgagatg ttgcgtaaca gggccagaag gctagactac
    22021 aaaataatgc gttgatgatg gaggcactgt ggaagcgatt aagggatcgg acgttaatgt
    22081 cccggatgca gtatttgcct ggatgctgga tggtagaggc ggcgttaaac cgctggaaaa
    22141 tacagatgtg attgatgaag cgcatccctg ctggctccac cttaattatg tacaccatga
    22201 tagcgcccaa tggctggcga caacaccgct gcttcccaat aacgtacgtg atgcgctggc
    22261 gggcgagagc acgcggcccc gagtcagccg tctcggtgaa ggcacgctga ttacattgcg
    22321 ctgtataaac ggcagcaccg atgaacgccc cgatcaactg gtcgccatgc gtgtatatat
    22381 ggacgggcgg ttaattgttt cgacccgaca acgcaaagtg ctggcgctgg acgatgtggt
    22441 gagcgatctg gaagagggca cgggtccgac cgattgcgga gggtggctgg tggatgtgtg
    22501 cgatgcgttg accgatcatt ccagcgaatt tatcgagcag ctgcacgata aaattatcga
    22561 ccttgaagat aatctccttg atcagcaaat tccaccgcgt ggattcctgg ctctgctgcg
    22621 caaacaatta atcgtgatgc gtcgctatat ggcaccgcaa cgtgatgttt atgctcgtct
    22681 tgccagtgaa cgtttgccgt ggatgagcga tgaccaacgc cgtcggatgc aggatattgc
    22741 cgatcgcctt gggcgcggtc ttgacgaaat cgacgcctgt atagcacgga ctggcgtgat
    22801 ggcggatgaa atcgctcagg tgatgcagga aaatttagct cgtcgtacct atacaatgtc
    22861 attgatggca atggtctttt tacccagtac ctttctgaca gggttatttg gcgtcaacct
    22921 tggtgggatc cctggcggcg ggtggcaatt cggattttca attttttgta ttctgttagt
    22981 tgttcttatt ggtggtgttg ctttatggtt gcaccgtagt aaatggttgt aacaaaagca
    23041 atttttccgg ctgtctgtat acaaaaacgc cgcaaagttt gagcgaagtc aataaactct
    23101 ctacccattc agggcaatat ctcccttgca ggtgaatgca acgtcaagcg atgggcgttg
    23161 cgctccatat tgtcttactt ccttttttga attactgcat agcacaattg attcgtacga
    23221 cgccgacttt gatgagtcgg cttttttttg cctgttattt atcagcgtct accctttaag
    23281 agtccaccca atgaccggag ggaaatatga cgacacttat ttatttgcaa attcctgtcc
    23341 ctgaaccgat tcctggcgat cctgttccag tgcccgatcc gatccctcgc ccgcaaccca
    23401 tgcctgaccc accacctgat gaagaaccga ttaaattgtc gcatcgtgag cgtagatctg
    23461 cgaggatacg cgcctgctaa ctttgcgtcg atgaccacga gaatagattg tgaccgcttt
    23521 ttctaccctg aatgttttgc ctcccgccca actcacgaac cttaatgagt tgggttattt
    23581 aaccatgacg ccggtgcagg ccgccgcgct tccggcgatc cttgccggaa aagatgttcg
    23641 cgtgcaggcg aaaaccggca gcggcaaaac ggcggctttt ggcctcggct tgttacagca
    23701 aattgatgcg tcgctatttc aaacccaggc tttagtgctg tgtcctacgc gtgaactggc
    23761 ggatcaggtg gcaggtgaat tgcgtcggct ggcgcgtttt ctgccaaata ccaaaatttt
    23821 gacgttgtgc ggtggtcaac cgttcggtat gcagcgtgat tcgttgcaac atgcgccgca
    23881 tattatcgtg gcaacgccgg gtcgtttgct ggatcacttg caaaaaggca cggtatcact
    23941 ggatgcgctg aatactttgg tgatggatga ggccgaccgc atgctggata tgggatttag
    24001 cgatgccatt gatgatgtca tccgttttgc gcctgcatct cgacagacgc ttctgttttc
    24061 ggcaacctgg ccggaagcca tcgccgcaat cagcggacga gtgcaacgcg atcctttggc
    24121 gattgaaatt gactcaacag atgctttgcc acccattgaa caacaatttt atgagacatc
    24181 cagtaaaggc aaaattcctc tgttgcaacg gttattaagt ttgcatcagc catcctcttg
    24241 tgtggtgttt tgcaatacca aaaaagattg ccaggctgtc tgcgacgcgc tgaatgaagt
    24301 agggcaaagt gcattgtcgt tacacggcga tctggagcaa cgcgatcgcg atcagaccct
    24361 ggtacgtttt gctaacggta gcgcccgtgt actggttgcg actgatgttg ctgcgcgtgg
    24421 tctggatatt aaatcgcttg agctggtggt gaactttgag ctggcgtggg accctgaagt
    24481 tcatgtacat cgcatcggcc gtacagctcg tgcaggaaat agcggtctgg cgatcagttt
    24541 ctgtgctccg gaagaagcac agcgggccaa tatcatttct gacatgttgc agataaaact
    24601 taactggcaa acgccgccag ctaatagttc cattttgccg ctggaagcag aaatggcaac
    24661 gttgtgtatc gatggcggga aaaaagccaa aatgcgcccg ggtgatgtat taggtgcgct
    24721 gacaggagac atcgggcttg atggcgcaga tattggcaaa atcgccgtgc atccggcgca
    24781 tgtctatgtc gcggtgcgtc aggctgttgc tcataaagca tggaaacagt tacagggcgg
    24841 gaagattaaa ggaaaaacgt gccgggtgcg gttattaaaa taatgaaatg ttgaattgcc
    24901 gggtgcaaga gtaaacatct tatgcgggat tgccggatgc gacgctggcc gcgtcttatc
    24961 cggcctccat aagagtagcc cgatacgctt gcgcatcggg cgctatcctg gttatttcac
    25021 ttcaaccaca ttcagccgta actcatccaa ctgattttca tcttcttctg gctgccagcc
    25081 cgccggttgt agtgggatct cttcgcgatc aaacgccaga tcacccccgt taaccacttc
    25141 agaaccgtgg gtaatgcctt tgaaatcgaa caggttggta tcgcacagat gcgacggcac
    25201 cacattctgc atcgcgctga acatcgtctc gatacgccct ggataacgtt tatcccagtc
    25261 acgcaacatg tcagcaatca cctgacgttg caggttaggc tgtgaaccgc acaggttgca
    25321 cggaataatc gggaacgctt ttgcatcggc aaatcgctga atatctttct cgcggcagta
    25381 ggccagcgga cgaataacga tatgtttgcc atcatcgctc atcagtttcg gaggcatacc
    25441 tttcatcttg ccgccgtaga acatatttaa gaacaacgtt tgcaggatat cgtcacgatg
    25501 gtgacccaac gcgatcttcg tcgcccccag ttccgttgcg gtacgataaa ggataccgcg
    25561 acgaaggcga gaacacagtg agcaagtggt tttgccctct ggaatcttct ctttcacgat
    25621 accgtaagta ttctcttcaa caatcttgta ctcaacgccc agcttttcaa gatactcggg
    25681 cagaacgtgt tccgggaagc ccggttgctt ttgatcgagg ttaacagcca ccagcgaaaa
    25741 attgattggc gcgctttgct gcaaattgcg cagaatctcc agcatggtat agctgtcttt
    25801 acccccggag aggcaaacca tgatgcgatc gccttcttca atcatattga agtcagcaat
    25861 ggcttcgccc acgttacgac gcagacgttt ttgtaatttg ttcaggttgt attgttcttt
    25921 ctttgtaatt tgttgatttt cttgcattat ttcagttctc tggtactaaa tggggcaaat
    25981 tgggggcaaa ctttgcaact acgataaccg cgcattcaac atggctatct gttcgtcgtt
    26041 catgtcatca atccacatac cgtaaatttc atacaccatc tgcgcagttt catgccccat
    26101 ttggctggct ataaatgccg ggttcgctcc tgccgtcaac agccagcagg caaaagtatg
    26161 ccgcgtatgg tacggattac ggcggcgaat accagcacgt tttactgctg cattccacct
    26221 tgcccccaaa ctgcttaccg agtaataagg tttttgtttt ccgttacaca ccctgggcat
    26281 gaaaacaaaa tgcagttttt gcttttcggt tctgccgtac tcccgatgat aaaaggtgat
    26341 ttcgcttttg cgatgatgcc cggtcagttt gtattgctcc ttcagtgctt caagagcagg
    26401 ctgcagtagt gttactgttc ggatcccggc atttgttttt gggggaccga acatatcaag
    26461 tatcgtcagg tttcttctga cattcactat tcccttttcg agatccacat cctcccacgc
    26521 cagagctgcc agttccccgt gacgaagtcc tgagtaaacg gcaaatttcc acaagttctg
    26581 gctctgtcct ttttcacttt ccattaatgc attgaattct gttttagata acggatcagg
    26641 ctttattctg tttcgctgta atttttttac tccttcaaat ggtttggttg atataaatcc
    26701 cgac
//
LOCUS       DAEVZA010000062        26666 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEVZA010000062 DAEVZA010000000
VERSION     DAEVZA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.062"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..172)
                     /locus_tag="LAF92_002605"
     CDS             complement(<1..172)
                     /locus_tag="LAF92_002605"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="catalyzes the formation of propanoyl-CoA from
                     methylmalonyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBI9791594.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPS"
     gene            complement(183..1178)
                     /gene="meaB"
                     /locus_tag="LAF92_002606"
     CDS             complement(183..1178)
                     /gene="meaB"
                     /locus_tag="LAF92_002606"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBI9791595.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(1171..3315)
                     /gene="scpA"
                     /locus_tag="LAF92_002607"
     CDS             complement(1171..3315)
                     /gene="scpA"
                     /locus_tag="LAF92_002607"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBI9791596.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRGVLNLISQHHD"
     gene            complement(3519..4412)
                     /gene="argP"
                     /locus_tag="LAF92_002608"
     CDS             complement(3519..4412)
                     /gene="argP"
                     /locus_tag="LAF92_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBI9791597.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            4554..4739
                     /locus_tag="LAF92_002609"
     CDS             4554..4739
                     /locus_tag="LAF92_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791598.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            4795..5454
                     /gene="rpiA"
                     /locus_tag="LAF92_002610"
     CDS             4795..5454
                     /gene="rpiA"
                     /locus_tag="LAF92_002610"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBI9791599.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            5710..6942
                     /gene="serA"
                     /locus_tag="LAF92_002611"
     CDS             5710..6942
                     /gene="serA"
                     /locus_tag="LAF92_002611"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBI9791600.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            7171..7230
                     /gene="ibsC"
                     /locus_tag="LAF92_002612"
     CDS             7171..7230
                     /gene="ibsC"
                     /locus_tag="LAF92_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBI9791601.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            7563..7622
                     /locus_tag="LAF92_002613"
     CDS             7563..7622
                     /locus_tag="LAF92_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI9791602.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(7723..8325)
                     /locus_tag="LAF92_002614"
     CDS             complement(7723..8325)
                     /locus_tag="LAF92_002614"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBI9791603.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(8346..8529)
                     /gene="ssrS"
                     /locus_tag="LAF92_002615"
     ncRNA           complement(8346..8529)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LAF92_002615"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(8571..8900)
                     /gene="zapA"
                     /locus_tag="LAF92_002616"
     CDS             complement(8571..8900)
                     /gene="zapA"
                     /locus_tag="LAF92_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBI9791604.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            9068..9646
                     /gene="ygfB"
                     /locus_tag="LAF92_002617"
     CDS             9068..9646
                     /gene="ygfB"
                     /locus_tag="LAF92_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBI9791605.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            9672..10997
                     /gene="pepP"
                     /locus_tag="LAF92_002618"
     CDS             9672..10997
                     /gene="pepP"
                     /locus_tag="LAF92_002618"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBI9791606.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            10994..12172
                     /gene="ubiH"
                     /locus_tag="LAF92_002619"
                     /gene_synonym="visB"
     CDS             10994..12172
                     /gene="ubiH"
                     /locus_tag="LAF92_002619"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBI9791607.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKALGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            12195..13397
                     /gene="ubiI"
                     /locus_tag="LAF92_002620"
                     /gene_synonym="visC"
     CDS             12195..13397
                     /gene="ubiI"
                     /locus_tag="LAF92_002620"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBI9791608.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYN
                     HLGHIIENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(13471..13554)
                     /locus_tag="LAF92_002621"
     CDS             complement(13471..13554)
                     /locus_tag="LAF92_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791609.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            13845..14939
                     /gene="gcvT"
                     /locus_tag="LAF92_002622"
     CDS             13845..14939
                     /gene="gcvT"
                     /locus_tag="LAF92_002622"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBI9791610.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            14963..15352
                     /gene="gcvH"
                     /locus_tag="LAF92_002623"
     CDS             14963..15352
                     /gene="gcvH"
                     /locus_tag="LAF92_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBI9791611.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            15471..18344
                     /gene="gcvP"
                     /locus_tag="LAF92_002624"
     CDS             15471..18344
                     /gene="gcvP"
                     /locus_tag="LAF92_002624"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBI9791612.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQELLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            18610..19353
                     /locus_tag="LAF92_002625"
     CDS             18610..19353
                     /locus_tag="LAF92_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI9791613.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(19410..20843)
                     /gene="bglA"
                     /locus_tag="LAF92_002626"
     CDS             complement(19410..20843)
                     /gene="bglA"
                     /locus_tag="LAF92_002626"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBI9791614.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            20888..21199
                     /locus_tag="LAF92_002627"
     CDS             20888..21199
                     /locus_tag="LAF92_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBI9791615.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            21363..22022
                     /locus_tag="LAF92_002628"
     CDS             21363..22022
                     /locus_tag="LAF92_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBI9791616.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(22218..23198)
                     /gene="ygfZ"
                     /locus_tag="LAF92_002629"
     CDS             complement(22218..23198)
                     /gene="ygfZ"
                     /locus_tag="LAF92_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBI9791617.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            23441..23707
                     /gene="sdhE"
                     /locus_tag="LAF92_002630"
     CDS             23441..23707
                     /gene="sdhE"
                     /locus_tag="LAF92_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBI9791618.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            23688..24095
                     /locus_tag="LAF92_002631"
     CDS             23688..24095
                     /locus_tag="LAF92_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBI9791619.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(24135..24656)
                     /gene="fldB"
                     /locus_tag="LAF92_002632"
     CDS             complement(24135..24656)
                     /gene="fldB"
                     /locus_tag="LAF92_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBI9791620.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            24768..25664
                     /gene="xerD"
                     /locus_tag="LAF92_002633"
     CDS             24768..25664
                     /gene="xerD"
                     /locus_tag="LAF92_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBI9791621.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            25689..26399
                     /gene="dsbC"
                     /locus_tag="LAF92_002634"
     CDS             25689..26399
                     /gene="dsbC"
                     /locus_tag="LAF92_002634"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBI9791622.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            26405..>26666
                     /locus_tag="LAF92_002635"
     CDS             26405..>26666
                     /locus_tag="LAF92_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="5'-3' single-stranded-DNA-specific exonuclease;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBI9791623.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATS"
BASE COUNT         6257 a         6765 c         7218 g         6426 t
ORIGIN      
        1 cactcggcgc gcgcaaaatg atacagcgaa tttccggccg gttgagatcg cttaatgcct
       61 gcataagatc atcaataaag actttactta aggcattaag tttgcggcca tagttaaact
      121 caatgaccgc cactttgttg atagtgacaa cgttaacata ctgataagac ataaaaattc
      181 ctttaatcaa aatattgcgt ctggataaat tcactgagct gccgcaggcc ggtgcgcggt
      241 gagagcgtat tgtttttgac cgctaaaagc gtctggcggt aatagcgatc gaaatcttca
      301 ttcgcgaaca ggtgattcag tacttcttct tcggtctgct tacgcagcca ttccaccgat
      361 tgttgttgcc gcacttgttg taaacgacca ctggcagtta gcgcggtttt gaagtcgatg
      421 atggcgtgcc agatctcatc gattccacgt ttttccagtg cgctacaagt cagaacccgt
      481 ggctgccatt cgtcgtattt acgtcgcaga atatgcaggg cactctcgta catatgccgg
      541 gcaatggcga cattggtatg gttatcgcca tcgtctttgt tgataacgag cagatcagcc
      601 acttccatca gccctttttt aatgccctgc agatcatcgc cgccaccggc aatttgcaac
      661 gagataaaac agtccaccat gcgggcgact tccgtttccg actgcccgac gccaaccgtt
      721 tcgacaatca ctacgtcata acccgccgct tcgcataaca gcattaattc ccgcgctcgc
      781 tgactggcac cgcccagatg accggaggat ggtaccgggc gaataaacgc cgcttcggca
      841 cgcgccaggt cattcatgcg ggttttatcc ccgagaatgc taccgccagt gaccgggctg
      901 ctgggatcga ccgcaataac cgcgaccttt aatccctcac gaatcaacaa catgccaaag
      961 gcttcaagaa aggtactttt ccccgcgccg ggtgtgccgg taacgcccag tcgcagggcg
     1021 ttaccgcagt acggcataat ggcatcaagc agctgcgtac ttagtgcctg atgacgcggg
     1081 tgacggcttt ccaccagcgt catggcctgg gcgagtgtgg cacgctcacc ctgacgtaag
     1141 cggcgaatac tttctgccag cgtggcttca ttaatcatga tgctggctta tcagattcag
     1201 tacgccgcgc acgctgtcga gcataggtgt acctggacca taaatcgccg ccacgccgcg
     1261 ctcttgcagg aaggcgtaat cctgcggtgg aatgacgcca ccggcgacta cgcagatatc
     1321 ttcgcgtccc cattttttca gcgcttcgac cagttccggg atcagcgttt tatgaccggc
     1381 agccagtgag gatgcgccca ctacgtgaac gtcattttct actgccaggc gggcgatctc
     1441 ttcaggtgta gagaacatcg ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct
     1501 ggcgatcact ttcgcgccgc gatcgtgtcc atcctggccc atcttagcga tcagaatgcg
     1561 cgggcgacga ccattgtcgg caaggaactg ctccgtttgc gcaacaatgg catcgaactc
     1621 ggaggccgat ttctccgact gatgatagct ttgcgcaatt acgccggtaa cacactggct
     1681 tggcaccaga taacggtcga aagcggcttc cagcgcatcg gaaatttcac ccagggtggc
     1741 gcgaacgcga gcggcattaa cagcggcagc cagcaggttt tcgttatgct gtgcggcgtg
     1801 agtcagggcg ttcaacgcgg cggttacggc ggcatcatca cgggtggcgc gaatgcgttc
     1861 cagcgaagca atttgctcgt tacgcaccat cacgttgtcg atctcaagta catcggtttc
     1921 gtcttcgtga tccagtttgt acttgttgac accaacgatg acacgcttgc cctggtcgat
     1981 cagcgactgt tcgcgcgctg aggcctcttc gatcattcgt tttggcagac ctgcttcgat
     2041 cgctttcgcc atgccaccgg cttcgtcgat ctgttggata atagctctgg cttgtttgac
     2101 gatttgatcg gtcagcgact caatgtaata ggatccggcc agtggatcga cggtgcggca
     2161 gagttctgat tcttcctgga tgatgatctg ggtgttgcgg gcaatgcgtg ctgagaaatc
     2221 ggtaggcaaa ccaagcgctt cgtcaaaggc gttggtatgc agtgactgag taccgcccag
     2281 cgtcgcagcc agtgcttcaa tggtggtgcg gataacgttg ttatacggat cctgttcagt
     2341 caggctccag cctgaggtct ggcagtgggt acgcagcgcc agtgatttcg ggtcctgtgc
     2401 gccaaatcca ctgaccgctt cgctccataa ataacgtgcc gcacgcaaca tggcgacgtt
     2461 cataaacaga tccatgccga taccgaagaa gaacgacagg cgaggagcga agtcatcaat
     2521 tttcagtccg gcagagattg ctgctttgat gtactcaatc ccatcagcga gcgtaaatgc
     2581 tacctgctgc acgcagttgg cacccgcttc acccatgtgg taaccgctga tactgatggt
     2641 attaaatcgc ggcatgttgc cggaacacca ggcgatgatg tcggcgataa tgcgcattga
     2701 cggttttggc gggtaaatat aggtgttgcg gcagaggtac tctttgagaa tatcgttttg
     2761 aatggtgccg gtcagtttat caggtgtaac accttgctct tctgcggcga cgatataaaa
     2821 cgccagtact ggtagcactg cgccattcat ggtcatcgaa accgacattt tatccagcgg
     2881 gatctggtcg aacaggactt tcatatcttc cacggtgtcg atagcgacgc ccgctttgcc
     2941 gacgtcaccc gccacgcgtg ggttatcgga gtcgtagcca cggtgggtgg caaggtcaaa
     3001 cgcaacggaa agaccttttt gcccggcggc caggttacgg cgataaaaag cgttggactc
     3061 ttttgctgtt gaaaaaccag catactgacg gatggtccac ggttgggcgg tatacatagt
     3121 ggcacgcggg ccacgaacgt agggcggcaa accaggaagg gtacctgtca cctccagatt
     3181 atcgagatcg gcttcggtat acagcggctt gatggcgatc ccttccgcgg tttgctgaac
     3241 cagcgagtcg acagttttct cccgacggct caattccttg ttggcaagct gttgccactc
     3301 ctgcacgtta gacattattt gctccgtaaa acgcaatcaa aatgagggta tttgcgccta
     3361 cggtgagctt tttacgtggg tttgtcgcca gcaaaaaaaa tgcattcaga cgtcgttttt
     3421 tgccgcctga gatttgtcta ttttgtgatg tgattcaagg aatacaaaaa gaagtgaggg
     3481 aaatgcctga tatgtgctat caggcattat ttgatggatt aatcctgacg aaggactttg
     3541 tgaccataat cgagtaacgc atcagtgact ttacgcatca tgcggctttc aggagcaaag
     3601 cggtgccagt agagcatccg tcgttgaaat agcccaggcg ttaagtcaat cagttcaccg
     3661 ctggccagct ctttctcgat ttgcaggtgc gggatcatac agcaggtggt gccctggcga
     3721 gcaagttgta cgaacgcttc tgaagaatta acgatatggc agggtacgct gcctggaggc
     3781 agatcgaagt tttgctgcaa aaaggcctgg tgcatatcgt caagatggtc aaacgcgacc
     3841 actggcgctt tcagtaatgc cgaacgcgtt acgccgttag ggaaatattt ttcggcaaag
     3901 ggttttgagc tgacgaacag atagtcgagc gcaccgagct tatcgacaag acaactcggc
     3961 agcgcctgat gttgaatact caccgcgccg accacttcgc cgcggcgcag acgttcctga
     4021 gtgcgggttt catcttctac ctgcaagttg aggcggatag gcgaatcagc caacacagga
     4081 gccagtgccg gaagcaacca cgtcgccaga ctgtcggcgt tgaccgccag tgaaagcagc
     4141 agcggagtcg aaccggtttg ttcatcgccc agccactctt cttccagcaa ctccacctgg
     4201 cgcagcagtg ccagcagttt ttgcccttgt tccgtcgggc gcggcggtac ggtacgcacc
     4261 aacagcggct gcccgaacat attttccagt tgcttaatgc gctgtgagac ggctgattgt
     4321 gtaatgcaca gcttttgtgc cgcgcgctca aatcctcgtt cacgtatcac cgcatccagt
     4381 gcctgtaatg ttctgtagtc cgggcgtttc attgctgttg tctgctcctg gtaaatgctt
     4441 tttcctgcac tatgacacaa ttttgtgtca ggttgcatat accgaagccg tcggcgggta
     4501 acatttgtat tcgtggttaa acgcgcagct taaaagatca gggatttgcg ctaatgcatt
     4561 tcgctcagcg agttcgcgcc ctggttgtgt tgaatggcgt ggcgttattg cctctgttcg
     4621 caccgtggag cggcataccc agaccgttgt atgctttatt tgcggggcga aaggggatgc
     4681 ccgccattgc gcgatatttt atggatgagt taaccacgcg gcttgccaac ggggtctgaa
     4741 tcgctttttt tgtatataat gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg
     4801 caggatgaat tgaaaaaagc agtaggatgg gcggcacttc agtatgttca gcccggcacc
     4861 attgttggtg taggtacagg ttccaccgcc gcacacttta ttgacgcgct cggtacaatg
     4921 aaaggccaga ttgaaggggc cgtttccagt tcagatgctt ccactgaaaa actgaaaagc
     4981 ctcggcattc acgtttttga tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc
     5041 gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt
     5101 gaaaaaatca ttgcttcggt tgcagaaaaa tttatctgta ttgcagacgc ttccaagcag
     5161 gttgatattc tgggtaaatt cccgctgcca gtagaagtta tcccgatggc acgtagtgca
     5221 gtggcgcgtc agctggtgaa actgggcggt cgtccggaat accgtcaggg cgtggtgacc
     5281 gataatggca acgtgatcct cgacgtccac ggcatggaaa tccttgaccc gatagcgatg
     5341 gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg gcttgtttgc taaccgtggc
     5401 gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa ccattgtgaa atgatctgac
     5461 gggggaacct cccccgttaa aaaaattctc ttcattaaat ttggtgacat gtgtcacgct
     5521 tttaccaggc aattgtcgat tgctctaaat aaatcctcta aaccagcata ttcatccaag
     5581 aattaccttt gcgtgatatt tcctcaacat cgcgacgcaa acgttcatat tgccgcaata
     5641 ttattttttg atatgttgaa aggcggatgc aaatccgcac acaacatttc aaaagacagg
     5701 attgggtaaa tggcaaaggt atcgctggag aaagacaaga ttaagtttct gctggtagaa
     5761 ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg gttacaccaa catcgaattt
     5821 cacaaaggcg cgctggatga tgaacaatta aaagaatcca tccgcgatgc ccacttcatc
     5881 ggcctgcgat cccgtaccca tctgactgaa gacgtgatca acgccgcaga aaaactggtc
     5941 gctattggct gtttctgtat cggaacaaac caggttgatc tggatgcggc ggcaaagcgc
     6001 gggatcccgg tatttaacgc accgttctca aatacgcgct ctgttgcgga gctggtgatt
     6061 ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc gcaccgtggc
     6121 gtgtggaaca aactggcggc gggttctttt gaagcgcgcg gcaaaaagct gggtatcatc
     6181 ggctacggtc atattggtac gcaattgggc attctggctg aatcgctggg aatgtatgtt
     6241 tacttttatg atattgaaaa caaactgccg ctgggcaacg ccactcaggt acagcatctt
     6301 tctgacctgc tgaatatgag cgatgtggtg agtctgcatg taccagagaa tccgtccacc
     6361 aaaaatatga tgggcgcgaa agagatttcg ctaatgaagc ccggctcgct gctgattaat
     6421 gcctcgcgcg gtactgtggt ggatattccg gcgctgtgtg atgcgctggc gagcaaacat
     6481 ctggcggggg cggcaatcga cgtattcccg acggaaccgg cgaccaatag cgatccattt
     6541 acctctccac tgtgtgaatt cgacaacgtc cttctgacgc cacacattgg cggttcgact
     6601 caggaagcgc aggagaatat cggcctggaa gttgcgggta aattgatcaa gtattctgac
     6661 aatggctcaa cgctctctgc ggtgaacttc ccggaagtct cgctgccact gcatggtggt
     6721 cgtcgtctga tgcacatcca cgaaaaccgt ccgggcgtgc taactgcgct gaacaaaatc
     6781 ttcgccgagc agggcgtcaa catcgccgcg caatatctac aaacttccgc ccagatgggt
     6841 tatgtagtta ttgatattga agctgacgaa gacgttgccg aaaaagcgct gcaggcaatg
     6901 aaagctattc cgggtaccat tcgcgcccgt ctgctgtact aattcccctt ctctgaaaat
     6961 taacgggcag gtcactgact tgcccgtttt tttatccctt ctccacaccg ttgccgcgtt
     7021 gcgcattcta ctttgcgagt ctgctccaga aaataaatca aagtcagatc aatgcgttgt
     7081 gtttatttaa tggggattta cgatggcggg acagcatggg gctgtaacgg taaagccctc
     7141 accgaagcga gggcttaaag gagaagggtt atgatgcgac ttgtcatcat actgattgta
     7201 ctgttactga taagtttcgg agcttattaa cagtcagtct caggggagga gtcatcctcc
     7261 cttaccctta ctcactaaat taggtcaaag aatcaacgat gtcaatcagg gcgatgcggg
     7321 gtgcatcgcc ctgaaccgaa ctttttgata gccacttcat cgcccacgtt tactgccgta
     7381 atagatcctt tttcccaaaa attatctctg ttttgcatgg ggtttttcga gcacgctcga
     7441 agaaaagatt taaaggtaga tcaatgcgtt gtggttattt aaagagagct tacgatagct
     7501 gggcagcatg gggctgtaac ggtaaagccc tcaccgaagc gagggcttga aggagaaggg
     7561 ttatgatgcg acttgccatc atactgattg tactgttact cataagtttc ccagcttatt
     7621 aacagtcagt cacaggggag gagcaatcct cccttaccct tactcactaa attaggttaa
     7681 agaatcaacg atgtcaatca gggcgatgca ggtgtatcgc ccttaccact cccagacttt
     7741 cgacggtgta accaccgcag gaagagggat atcccactct tcaacgggga gtttttccac
     7801 caactgacaa tcatgcgcat aacccaccgg ttgcgtttta tagtgctgcc agttttgtaa
     7861 ggtccgatca taaaaaccac cgcccattcc caggcgctga ccgtactcat caaaggcgac
     7921 cagcggtgtg atcagcacgt ctaatcggga aaggggtagc acgtcacgca catccagttt
     7981 tggctcatgg atcttcaacc tgttcatcac cagttcgctt tgcggatggt aattcaggaa
     8041 cagcaaatta ccggcactaa agggatgcaa aactggaaga tatacgcgct taccggcgcg
     8101 ccagagttgt tctattagcg gctgggtgtc gagttcacca tcaaaagaga ggaatacagc
     8161 gaccgtatgc gccatcacca ccgggggata agtcatcatc cgggtagcgg cttgttgacc
     8221 catttcctgc tgttgttccg gcgttaacgc acgacgacgt tgccgaatca ttttgcggat
     8281 ttcttgtcgg gataatgtca gtgggagttc tggtagttgc gtcatggctg gtgccagata
     8341 agaagggaat ctccgagatg ccgccgcagg ctgtaaccct tgaacccttg gttcaaggtg
     8401 aatgtgtcgt cgcagtttta aggcttctcg gacggaccga gcatgctcac caaccgcgga
     8461 gcgccacatt cttgtggtat gaaatatcgg ctcaggggac tggcccgctt gcgaacatct
     8521 cagagaaatt ttgtcttcac ggttactcta ccacagtaaa ccgaaaagtg tcattcaaag
     8581 ttttggttag ttttttcggt gatgcgacct tgttcaagta acgcttgttc tatggtctgc
     8641 tgcagcatcc gaatacgctg ttccatactt gccgcgtagt cacgagtctt cgctttttct
     8701 tgcgctaact catagctgat attcaatgcg gcaatgaaga ccaactgttc agtatttgtg
     8761 actctagtgc gttctttcag atcttgcaac cgttggttca gatcgtccgc tgcctgattc
     8821 aacgcatccc tttggtcagg cgggcagttc acacgcagtg aacggccaaa aatttggata
     8881 tcgacgggtt gtgcagacat gccaccttcc tgctgattga ctgcgctgcc ttcgtcttta
     8941 gacccatggt ctgcgaaggg gcgacactat agctaccctg atgagaagag acaagccctt
     9001 ttctggtcca ccagggccca aagtggtagc atatcatgaa tattcctccc tttgacgacg
     9061 aatgcttatg tctatacaga acgaaatgcc tggttacaac gaaatgaacc agtatctgaa
     9121 ccaacaaggg acgggtctga cccctgctga gatgcatggt ttaatcagtg ggatgatatg
     9181 tggcggtaac gatgacagct catggctacc gctacttcac gacctgacga acgaaggcat
     9241 ggctttcggt catgagctgg cacaggcact gcgtaaaatg cactctgcca ccagcgatgc
     9301 cctgcaggat gacggcttcc tttttcagct ttatctgcct gatggcgatg atgtcagcgt
     9361 tttcgatcgg gctgatgcat tggcaggttg ggtcaatcac ttcctgcttg gtcttggcgt
     9421 tacgcaaccg aagctggata aagtgaccgg cgaaaccggt gaagctatcg acgatctgcg
     9481 taacattgcg caactgggtt acgacgaaga cgaagatcag gaagagcttg aaatgtcgct
     9541 tgaagagatc atcgaatacg ttcgtgttgc cgcgctgtta tgccacgaca cctttactca
     9601 tccgcaaccg accgcgccag aagtacaaaa accgactcta cactaaaaac aaaaaacgta
     9661 aggagagtgt tatgagtgag atatcccggc aagagtttca gcgtcgccgt caggccctgg
     9721 tggagcaaat gcaacccggc agcgccgcgc tgatttttgc tgcaccagaa gtaacacgta
     9781 gcgccgacag cgaatacccc tatcgtcaga acagtgactt ctggtacttc accggcttta
     9841 acgaaccgga agcggtgctg gtgctgatta aaagcgatga cactcataac cacagcgttc
     9901 tgtttaaccg cgttcgcgac ctgacggcgg agatctggtt tggccgtcgc ttaggccagg
     9961 atgccgcgcc agagaaactg ggcgttgacc gcgcactggc attcagcgaa atcaatcagc
    10021 aactttatca actacttaac ggcctggatg tggtttacca tgcccagggc gaatatgcat
    10081 atgctgatga aatcgtgaac agtgcgctgg aaaaactgcg taaaggttcg cggcaaaatc
    10141 tcaccgcacc ggcaacgatg atcgactggc gtcctgttgt tcatgaaatg cgcctgttca
    10201 aatcgccaga agagattgcc gtactccgcc gcgcgggaga aatcaccgcc ctggcacata
    10261 cccgggcgat ggaaaaatgc cgtccgggaa tgttcgagta ccatctggaa ggcgaaattc
    10321 accacgaatt taaccgccac ggtgcgcgct atccgtccta taacaccatt gtcggcagcg
    10381 gtgaaaacgg ctgcattctg cactacaccg aaaacgagtg tgaaatgcgc gacggcgacc
    10441 tggtgttgat tgacgcgggt tgcgaataca aaggttacgc tggcgatatt acccgcacct
    10501 tcccggtcaa cggcaaattc actcaggccc agcgtgaaat ctacgacatt gtgctggagt
    10561 ctctcgaaac cagcttgcgc ctgtatcgtc cgggaacttc cattctggaa gtcaccggtg
    10621 aagtggtgcg catcatggtt agcggcctgg taaaactcgg cattctgaaa ggtgatgttg
    10681 atgaactgat cgctcagaac gcccatcgtc ctttctttat gcatggcctt agccactggt
    10741 tagggctaga tgtccatgac gttggcgttt atggtcagga tcgctcgcgc attctggaac
    10801 cgggcatggt actgaccgta gagccagggc tgtatattgc gccggatgca gaagtgccag
    10861 aacaatatcg cggtatcggc attcgtattg aagacgacat tgtgattacc gaaaccggta
    10921 acgaaaacct caccgccagc gtggtgaaaa agccggaaga aatcgaagcg ttgatggcag
    10981 ctgcgagaaa gcaatgagcg taatcatcgt cggtggcggc atggcgggcg cgacgctggc
    11041 gctggctatt tcccggttaa gtcacggggc gctgccggta catttgattg aagcgaccgc
    11101 gccagagtca catgctcatc cgggctttga tggacgagct attgcgctag cggcggggac
    11161 ctgtcagcaa ctggcgcgca tcggcgtctg gcaatctctg gctgattgcg caaccgccat
    11221 cactaccgtg catgtcagcg atcgtggtca cgctggattt gtcaccctcg ccgcagaaga
    11281 ttaccaactg gcggcgctgg gacaggttgt cgaattgcac aatgtcgggc aacggctgtt
    11341 tgcattgctg cgtaaagcac ttggcgtaac gctgcattgc ccggatcgcg tggctaacgt
    11401 tgcccgtact cagagtcacg ttgaagtgac gctggagagt ggcgagacgc tgacgggccg
    11461 cgtgctggta gcagctgatg gcacccattc agcgttagcc accgcgtgcg gcgttgactg
    11521 gcagcaggag ccttacgaac aactggccgt gattgccaac gttgcgactt ccgttgcgca
    11581 cgaaggccgc gcttttgaac gcttcacgca acatggcccg ctggcgatgt tgccgatgtc
    11641 tgacggacgc tgttcgctgg tctggtgtca tccactggaa cggcgcgaag aggtgctgtc
    11701 gtggagcgac gagaagtttt gccgtgaact ccagtcggcc tttggctggc gacttggaaa
    11761 aattacccac gccggtaaac gcagtgctta tccgctggcg ttaacccgcg ccgccagagc
    11821 tattacccat cgtaccgtgc tggtgggcaa tgcggcgcaa actctgcacc cgattgccgg
    11881 gcaagggttt aacctcggta tgcgagatgt catgagcctt gcggaaaccc tgactcaggc
    11941 gcatgagcgc ggagaagaca tcggtgatta cggcatattg tgccgctatc agcagcgtcg
    12001 acagagcgat cgcgaagcaa ccatcggcgt cacggacagc ctggtacatc tttttgccaa
    12061 ccgttgggca ccgctggttg tcgggcgcaa catcggactg atgacgatgg aattattcac
    12121 cccggcacgc gatgtgctgg cgcagcgcac cctcggttgg gtggcgcgtt gaaggcggtt
    12181 ttaaggaata aacaatgcaa agtgttgatg tagccattgt tggtggcggc atggtggggc
    12241 tggcggttgc ctgcggctta caggggagcg gcttacgcgt tgccgtactg gagcagcgcg
    12301 taccggaacc tctggcggcg gatgcaccac cacaactgcg cgtttcggct atcaatgccg
    12361 ccagcgaaaa attactcacc cgtcttggcg tctggcagga cattctctcg cgtagggcca
    12421 gctgttatca cggtatggaa gtgtgggaca aagacagttt tggtcacatt tcatttgacg
    12481 atcaaagcat gggctataac catcttgggc atatcattga aaactcagtg attcactacg
    12541 cgctgtggaa caaagcgcag cagtcgtcag atatcactct gttggccccc gcagaattac
    12601 agcaggtcgc ctggggagaa aacgaaacct tcctgacgct gaaagatggc agtatgttaa
    12661 cggcgcgtct ggtgattggc gcggacggcg ctaattcctg gttgcgcaac aaagccgata
    12721 ttccgctgac tttctgggat tatcagcatc acgcgctggt agcgaccatt cgcacggaag
    12781 aaccgcatga tgcggtggcg cggcaggttt tccatggcga aggcattctg gcctttttac
    12841 cgcttagcga tccgcatctt tgctcgattg tctggtcact gtcgccagag gaagcgcagc
    12901 ggatgcagca ggcaagtgaa gacgaattta atcgcgcgtt aaatattgct tttgataatc
    12961 gcctgggctt atgcaaggtt gagagtgagc gccttgtgtt cccactgacg gggcgttatg
    13021 cgcgccagtt tgccgcgcac cgtctggtgc tggtgggcga cgccgcgcat accattcacc
    13081 cgctggcggg gcagggagta aatctcggct ttatggatgc tgcagagctg attgctgaac
    13141 tgaaacggtt gcatcgtcag gggaaagaca tcgggcagta catttacctg cgccggtatg
    13201 agcgtagccg caagcacagt gcggcgctga tgctggctgg tatgcaggga ttccgcgatc
    13261 tgttttccgg taccaatccg gcgaaaaaac tgctgcgtga tattggtttg aaactggccg
    13321 acacgcttcc tggcgttaaa ccacaactta tccgccaggc aatgggatta aacgatttgc
    13381 ctgaatggct gcgttaaaaa tttctcttct gctgtttatt tgatacccat cacactttca
    13441 tctcccggtt ttttcgccgg gagattttcc tcatttgaaa taaactaatt tcacctccgt
    13501 tttcgcatta tattttctaa tgtcacgaat ttttgattta gtgttttttg acattttttc
    13561 agctcttaat attgccttat tcaaattaac tttctcatca catcatcttt atataaaaac
    13621 ttgtgtattt tttggttttt tattctgttg cgattttggc gttttttaac cataagctaa
    13681 tgtgatgatc aattttacct tatggttaac agtctgtttc ggtggtaagt tcaggcaaaa
    13741 gagaacgatt gcgttgggga ccgggagtgg ctccgatgct gggtttcgtg gtgataattt
    13801 caccatgaaa aagttgtcag ccccgcttat tcaatgagga caagatggca caacagactc
    13861 ctttgtacga acaacacacg ctttgcggcg ctcgcatggt ggatttccac ggctggatga
    13921 tgccgctgca ttacggttcg caaatcgacg aacatcatgc ggtacgtacc gatgccggaa
    13981 tgtttgatgt gtcacatatg accatcgtcg atcttcgcgg cagccgcacc cgggagtttc
    14041 tgcgttatct gctggcgaac gatgtggcga agctcaccaa aagcggcaaa gccctttact
    14101 cggggatgtt gaatgcctct ggcggtgtga tagatgacct catcgtctac tactttactg
    14161 aagatttctt ccgcctcgtt gttaactccg ccacccgcga aaaagacctc tcctggatta
    14221 cccaacacgc tgaacctttc ggcatcgaaa ttaccgttcg tgatgacctt tccatgatcg
    14281 ccgtacaagg gccgaatgcg caggcaaaag ctgccacact gtttaatgat gctcagcgtc
    14341 aggcggtgga agggatgaaa ccgttctttg gcgtgcaggc gggcgatctg tttattgcca
    14401 ccactggtta taccggtgaa gcgggctatg aaattgcgct gcccaatgaa aaagcggccg
    14461 atttctggcg tgcgctggtg gaagcgggcg ttaagccatg cggtctgggc gcgcgtgaca
    14521 cgctgcgtct ggaagcgggc atgaatcttt atggtcagga gatggacgaa actatttctc
    14581 ctttagccgc caacatgggc tggactatcg cctgggaacc ggcagatcgt gacttcatcg
    14641 gtcgtgaagc cctggaagtg cagcgtgagc atggcacaga aaaactggtt ggtctggtga
    14701 tgaccgaaaa aggcgtgctg cgtaatgaac tgccggtacg ttttaccgat gcgcagggca
    14761 accagcatga aggcattatc accagcggga ctttctcccc gacgctgggt tacagcattg
    14821 cgctggcacg cgtgccggaa ggtattggcg aaacggcgat tgtgcaaatt cgcaaccgtg
    14881 aaatgccggt taaagtgacg aaacctgttt ttgtgcgtaa cggtaaagcc gtcgcgtgat
    14941 ttactttttt ggagattgat tgatgagcaa cgtaccagca gaactgaaat acagcaaaga
    15001 acacgaatgg ctgcgtaaag aagccgacgg cacttacacc gttggtataa ccgaacatgc
    15061 ccaggagctg ttaggcgata tggtgtttgt tgacctgccg gaagtgggcg caacggttag
    15121 cgcgggcgat gactgcgcgg ttgccgaatc agtaaaagcg gcgtcagaca tttatgcgcc
    15181 agtaagcggt gaaatcgtgg cggtaaacga cgcactgagc gattccccgg aactggtgaa
    15241 cagcgaaccg tatgcaggtg gctggatctt taaaatcaaa gccagcgatg aaagcgaact
    15301 ggaatcactg ctggatgcga ccgcatacga agcattgtta gaagacgagt aacggcttta
    15361 ttcctcttct gcgggagagg atcagggtga ggaaaattta tgcctcaccc tcactctctt
    15421 cgtaaggaga gaggttcaca attcactgca cgtttcagga accatcgctc atgacacaga
    15481 cgttaagcca gcttgaaaac agcggcgctt ttattgaacg ccatatcgga ccggacgccg
    15541 cgcaacagca agaactgctg aatgccgttg gtgcacaatc gttaaacgcg ctgaccggcc
    15601 agattgtgcc gaaagatatt cagcttgcga ccccgccgca ggttggcgca ccggcgaccg
    15661 aatacgccgc actggcagaa ctcaaggcta ttgccagtcg caataaacgc ttcacgtctt
    15721 acatcggcat gggttacacc gccgtgcagc taccgccggt tatcctgcgt aacatgctgg
    15781 aaaatccggg ctggtatacc gcgtacactc cgtatcaacc tgaagtctcc cagggccgcc
    15841 ttgaagcact gctcaacttc cagcaggtaa cgctggattt gactggactg gatatggcct
    15901 ctgcttctct tctggacgag gccaccgctg ccgccgaagc aatggcgatg gcgaaacgcg
    15961 tcagcaaact gaaaaatgcc aaccgcttct tcgtggcttc cgatgtgcat ccgcaaacgc
    16021 tggatgtggt ccgtactcgt gccgaaacct ttggttttga agtgattgtc gatgacgcgc
    16081 aaaaagtgct cgaccatcag gacgtcttcg gcgtgctgtt acagcaggtt ggcacaaccg
    16141 gtgaaatcca cgactacacc gcgcttatta gcgaactgaa atcacgcaaa attgtggtca
    16201 gcgttgccgc cgatattatg gcgctggtgc tgttaactgc gccgggtaaa cagggcgcgg
    16261 atattgtttt tggttcggcg caacgcttcg gcgtgccgat gggctacggt ggcccacacg
    16321 cggcattctt tgcggcgaaa gatgaataca aacgctcaat gccgggccgt attatcggtg
    16381 tatcgaaaga tgcagctggc aataccgcgc tgcgcatggc gatgcagact cgcgagcaac
    16441 atatccgccg tgagaaagcg aactccaaca tttgtacttc ccaggtactg ctggcaaaca
    16501 tcgccagcct gtatgccgtt tatcacggcc cgattggcct gaaacgtatc gctaaccgca
    16561 ttcaccgttt gaccgatatc ctggcggcag gcctgcaaca aaaaggtctg aagctgcgcc
    16621 atgcgcacta tttcgacacc ttgtgtgtgg aagtggccga caaagcgggc gtactgacgc
    16681 gtgccgaagc ggctgaaatc aacctgcgta gcgatattct gaacgcggtt gggatcaccc
    16741 ttgatgaaac aaccacgcgt gaaaacgtaa tgcagctttt caacgtgctg ctgggcgata
    16801 accacggctt ggacatcgac acgctggaca aagacgtggc tcacgacagc cgctctatcc
    16861 agcctgcgat gctgcgcgac gacgaaatcc tcacccatcc ggtgtttaat cgctaccaca
    16921 gcgaaaccga aatgatgcgc tatatgcact cgctggagcg taaagatctg gcgctgaatc
    16981 aggcgatgat cccgctgggt tcctgcacca tgaaactgaa cgccgccgcc gagatgatcc
    17041 caatcacctg gccggaattt gccgaactgc acccgttttg tccgccggaa caggctgaag
    17101 gttatcagca gatgattgcg cagctggctg actggctggt gaaactgacc ggttacgacg
    17161 ccgtttgtat gcagccgaac tccggcgcac agggcgaata cgcgggcctg ctggcgattc
    17221 gtcattatca tgaaagccgc aacgaagggc atcgcgatat ctgcctgatc ccggcttctg
    17281 cgcacggaac taaccccgct tctgcacata tggcaggaat gcaggtggtg gttgtggcgt
    17341 gtgataaaaa cggcaacatc gatctgactg atctgcgcgc gaaagcggaa caggcgggcg
    17401 ataacctctc ctgtatcatg gtgacttatc cttctaccca cggcgtgtat gaagaaacga
    17461 tccgtgaagt gtgtgaagtc gtgcatcagt tcggcggtca ggtttacctt gatggcgcga
    17521 acatgaacgc ccaggttggc atcacctcgc cgggctttat tggcgcggac gtttcgcacc
    17581 tcaacctgca taaaactttc tgcattccgc acggcggtgg tggtccgggt atgggaccga
    17641 tcggtgtgaa agcgcatctg gcaccgtttg taccgggtca tagcgtggtg caaatcgaag
    17701 gcatgttaac ccgtcagggc gcagtttctg cggcaccgtt cggtagcgcc tctatcctgc
    17761 caatcagctg gatgtacatc cgcatgatgg gcgcagaagg gctgaaaaaa gcaagccagg
    17821 tggcaatcct caacgccaac tatattgcca gccgcctgca ggatgccttc ccggtgctgt
    17881 ataccggtcg cgacggtcgc gtggcgcacg aatgtattct cgatattcgc ccgctgaaag
    17941 aagaaaccgg catcagcgag ctggatattg ccaagcgcct gatcgactac ggtttccacg
    18001 cgccgaccat gtcgttcccg gtggcgggta cgctgatggt tgaaccgact gaatctgaaa
    18061 gcaaagtgga actggatcgc tttatcgacg cgatgctggc tatccgcgca gaaatcgacc
    18121 aggttaaagc cggtgtctgg ccgctggaag ataacccgct ggtgaacgcg ccgcacattc
    18181 agaacgaact ggtcgccgag tgggcgcatc cgtacagccg tgaagttgca gtattcccgg
    18241 caggtgtggc agacaaatac tggccgacgg tgaaacgtct ggatgatgtt tacggcgacc
    18301 gtaacctgtt ctgctcctgc gtaccgatta gcgaatacca gtaattcact gattcgacta
    18361 tcttctaaag gcgcttcggc gcctttttag tcagatgaca aagtacaaaa gcgctcagac
    18421 agtcccctca cccctttggg gagagggtta gggtgagggg aacaggccgg cactggcgcg
    18481 aatatttacc ctcaccccgg ccctctccct gaaagggcga ggggaaaagc gtgccaacat
    18541 tgaagattga gccagtttgt tagcaatctc aaagatacgt caacgaatta atttttctcg
    18601 gaaaaacaaa tggctatagc acttgtgacc ggtggcagtc gcggcatcgg gcgggcaact
    18661 gcattactgt tggcgcaaga agggtatacg gtggcggtta attatcagca aaacctccac
    18721 gcggcgcagg aagtgatgaa cttaataacg caagccggtg gcaaagcatt cgtgctccag
    18781 gcggatatca gcgacgaaaa ccaggtcgtt gcgatgttta cagcaatcga tcagcacgat
    18841 gaaccgctag cagcgctggt caataacgcc gggatcttgt ttacccagtg caccgttgaa
    18901 aaccttaccg cagagcgaat caaccgagta ctttccacca acgtgacggg atattttctc
    18961 tgctgccgcg aggcggtaaa acgcatggcg cttaaaaatg gtggcagtgg cggcgctatc
    19021 gtcaatgtct cttcggtggc ctcacggttg ggttcgccag gggaatatgt tgattacgcg
    19081 gcatcgaaag gggcgattga tacgttaacc accggactat cgctggaagt cgccgcgcag
    19141 gggatccgcg ttaactgcgt gcggccaggg tttatttata ccgaaatgca cgccagcggc
    19201 ggcgagcctg gacgcgtcga tcgcgttaag tcgaacatcc ccatgcagcg tggtggacag
    19261 gcagaagagg tcgcgcaggc cattgtctgg ctactaagtg ataaagcctc ttacgtcacg
    19321 ggaagtttta tcgatttggc gggcgggaaa taaaacaggg aagttgtctg accggatgca
    19381 acaagtattg catccggtac ttcatcgact taaagcttct cgccgttgct ggcaatcacc
    19441 tcttggtacc agttaaagct cttcttacgt gaacgcgaca tatcgccagc accgtcgtca
    19501 tgtttattca catagataaa gccgtagcgt ttgctgtact gcccggtggt gaacgatacg
    19561 cagtcgatgc agccccacgg tgtgtagccc atcagatcca cgccatcgta agtcaccgct
    19621 tttttcatct cttcgatatg ggcgcgcagg tagtcaatgc ggtagtcgtc gttgatgctg
    19681 ccatcttctt ccactttgtc gtaagcgcca aaaccgtttt cgacaataaa cagcggcctc
    19741 tgataacgct catacagttc gcaaagggca tagcgcagac ctactggatc aatctgccag
    19801 ccccagtcag acgctttaac atacgggttc ggtacgctgc cttcgaaacc agagatcgca
    19861 tcgccggtgc cgccttcggc cttcactgcg ttggtcatgt aatagctgaa accaagataa
    19921 tcgcaggtgc cttcacgcag tacatcgaga tcgccgtctt ccattttgat gttaaatccg
    19981 cgacgctccc actcgttcaa cacataggac gggtaatagc cgcgtagctg cacatcggta
    20041 aagacgtagc gttcgcgcat cgactcctga gcgaacatca catcgtccgg gttacagcag
    20101 tagggataga gcggcaccat cgccagcata cagccgactt tcatctccgg gttaatgcga
    20161 cgcgcagctt tcaccgccag ggcgctggcg acaaactggt gatgcagcac ctgatacatc
    20221 gtctcttccg ggttctcatg ctcggtatac accacgccgg agcagcagta accgaacagc
    20281 ggtgcacgcc agttacgctg gttgttaatt tcgttgaagg tcatccagta tttgacttta
    20341 tgcttgtagc gttcgaatac cacttccgcg aaacggacaa agaaatcaac gactttacgg
    20401 ttggtccagc taccgtattg ctgcaccaga tgcagcggca tttcaaagtg ggagagggtt
    20461 atcaccggtt cgatgttgta tttcagcagt tcatcgaaca tatcatcgta gaacttcagc
    20521 ccttcttcgt ttggctgagc ttcatcacct tttggaaaaa tacgcgtcca ggcgatggaa
    20581 gtccgaaaac atttgaagcc catttcggca aatagcttga tgtcttcctt gtagtgacca
    20641 taaaaatcaa cggcttcatg gtttggatag tatttgcctg gcaagacttc tttggtgatt
    20701 tcgcgcggca cgccgtgtgc gccaccggtc agaacgtcac aaatgctcgg ccctttgccg
    20761 cctttgttcc agccgccttc gacctgatga gcggcaactg cgccgcccca taagaaatct
    20821 ttcggtaagg tgagtttttt cactatcata ggctccagat gagtcaattc ttacaggaaa
    20881 tagtaccatg cagccaaacg acatcacttt ttttcaacgt ttccaggatg atattctggc
    20941 tgggcgtaaa accatcacca tccgcgacga gtctgaatcg cacttcaaaa cgggtgatgt
    21001 gcttcgtgtc gggcgttttg aagatgacgg ttatttttgc acgattgaag tcaccgcaac
    21061 ctcaaccgta acgctggata cgctgacgga aaaacatgca gagcaggaaa atatgaccct
    21121 gactgaactg ataaaggtca ttgccgacat ctatcccggt cagacacagt tttatgtgat
    21181 tgaatttaaa tgtctttaaa ttgaactcac gcctgttagt tgaaatttcg catttaattt
    21241 catgattttt aggagattag tctcaaatca gaatttattt tagctaacag gtgttcactg
    21301 gaactattct cagttacgct ggagaggtat acatgtgcgt gtatgtttct ccggagtaag
    21361 ttatggttca gaagcccctc attaagcagg gatattcgct ggcagaggaa attgccaaca
    21421 gcgtcagtca cggcattggg ttggtgtttg gtatcgttgg gctggtgttg ctactggttc
    21481 aggcggtgga tctcaatgcc agcgccacag cgataaccag ttacagcctc tatggcggca
    21541 gtatgatcct gctgttcctc gcttcgacgc tctatcacgc cattcctcat caacgggcaa
    21601 aaatgtggct gaagaaattt gaccattgcg ctatttacct gttgattgcc ggaacctaca
    21661 cgccgttttt gctggtgggg ctggattctc cgttagcgcg cgggttgatg attgttatct
    21721 ggagcctggc attgctgggt attctgttta aactgaccat cgcgcaccga ttcaaaattt
    21781 tatctctggt gacctatctg gcgatgggct ggctgtcgct ggtggtaatt tatgaaatgg
    21841 cagttaagct cgcggcgggc agcgttacct tactggcggt aggcggcgtg gtttattcgc
    21901 tcggggtgat tttctacgtc tgcaaacgca ttccatacaa ccatgccatc tggcacggct
    21961 tcgtgctcgg cggtagtgtg tgccactttc tggcgatcta tttgtatatt gggcaggcgt
    22021 aatgggtatg cctttgtagg cctgataaga cgcgccagcg tcgcatcagg catcggagcg
    22081 caaccgccgg atgcggcgtg aacgccttat ccgatctacg ggcctcgtcc ctgttgtgat
    22141 aaaggcatca aatcccagac atgataagac gcgtcagcgt cgcatcaggc gataaatgcc
    22201 tgatgcgata cagagattta ctcttcgagc gaatacggca gcggctcgat atgcagtgta
    22261 ttcgcatcgt cgcgtacgcg gaagatgctg tccggttcca tatcgttatt catgacgacc
    22321 tgtaccacga cctgaccatc ttccagtttt accgcagcca gcaccgtacc ggtacgacgc
    22381 cagttctcgc ccattttcag ctctaagtct tcaccagctt ccggcagtcg gctggcgcta
    22441 cctgccagca accagagcgc gcgtttgttg gcaccacgga attttgctcg cgccaccatc
    22501 tcttgtccgg tataacagcc tttcttaaag ctgataccgc ccagcgcctg gagattggtc
    22561 gcctgtggga taaactgccc gctgttggcg gcatcaatca ccgggaaacc cgcttcaatg
    22621 tttaatgcca gccactgttg gctattgttc agttccgctt caccgcgcag tttatcggtc
    22681 agcatattag cagtagcttc atcggttacg atcaggaaac gttctgccgg gtgttcaaac
    22741 catagcaaag tggtcgcgcc ttctttgact acctgttttt ctttcgaagg cagttcgcta
    22801 aagagatttg ccagcgcggc gcgcgcctga aaaccggcaa caccaagcag cacacgctcg
    22861 tcgtctggcg cgatggtcac tttagagaat accgcatatt ttttcagttc agtcagctgc
    22921 ggttcacgca cgctgcgccg ttcaatccat gcaaagccat cgccgtcgcg gaacagacgt
    22981 aaattgctcc acattttacc tttggcgtcg caatgggcgg cgagcaggtg ctgattttct
    23041 gtcatctggc tgacatctgc tgtcacctga ccctgcatat atttttcgct gtccgcgcca
    23101 gtaatggtgg caagcgccca gtcatcaagc gtcatcagcg tcagcggtaa acgggcagaa
    23161 gccgtaggct gacggggagg aaaaggtgta aaagccataa tagagtcctg agttgcttaa
    23221 cggcgtatta atgactaatg gtaaaagagc tatgaggcaa tgcaagtgct ttcactacgc
    23281 gatctgtgcc ttctgcgagg cgacttccaa aaaaatgaag ccattgaatt gttgtgaatt
    23341 aatttcgcga tcgcgatggt gtttactgat attcccgtaa aagaaggcga aaaacacgtt
    23401 acaatagccg ggtactggat tttcgtgaga caggaagaag atggacatta acaacaaagc
    23461 ccgcattcat tgggcatgcc gccgtggtat gcgcgaactc gatatttcaa tcatgccgtt
    23521 tttcgaacat gagtacgaca gcttaagcga tgacgaaaaa cgcatcttta ttcgtctgct
    23581 ggaatgtgac gatccggacc tgtttaactg gctgatgaat cacggtaaac cggccgatgc
    23641 agaactggaa atgatggtcc gactcatcca gacacggaac cgggaacgtg gtcctgtggc
    23701 aatctgattt gcgcgtctcc tggcgcgcac agtggctttc cttgctgatt catgggctgg
    23761 ttgccgctgt tattttactc atgccctggc cactcagtta caccccgtta tggatggtgt
    23821 tactttcgct ggtggtgttt gattgcgttc gcagccagcg gcgtattaat gctcgccagg
    23881 gggaaattcg cttgttgatg gacgggcgtt tgcgttggca agggcaggag tggtgcatcg
    23941 tcaaagcacc gtggatgatt aagagcggca tgatgctgcg tttacgttct gatggcggta
    24001 aacggcaaca tttatggctg gcagccgaca gcatggacga agccgaatgg cgggatttac
    24061 ggcggatttt gttgcaacaa gagacgcaaa gataaagatg acggcaaagc atatgcgcta
    24121 agccgtcagt gagatcaggc gtaatgctct gccatttcgt tgaggatttg ctcgcaccag
    24181 ctctgaatac gctcgtcgct aaggtcatac tggttagttt catccagcgc cagacccacg
    24241 aacagttgcc cgtcagcaat caccggtttc gggctggtaa attcatatcc ttccgttggc
    24301 cagtagccga cgaacttcac gcctttggtc gagagtttgt catgcagcat accgagcgca
    24361 tcgaggaacc actcgccgta tcccagttga tcgccaagcc catacagcgc aacaatttta
    24421 ccttcaaggt tcaggtcgtc gagctgatcc cagacggctt cccagtcttc ctggatttca
    24481 ccaaaatccc aggtcgggat acccagaatg agcacatcgt actgctccat taatttcggg
    24541 gagtcgtcct tgaggttatg taaggtcacc agttctgggc cgataatatc gcggattttt
    24601 tctgccgcca tttcggtgta acaggtgctg gaaccgtaaa aaagacccat attcataacg
    24661 taagcttctc gattctgctg tggcttttgc agctagtgta tcagaatcgc tttagtcacg
    24721 gcacaataag gcataatgga tcaaatgagt gaccataagg ggcgcaagtg aaacaggatc
    24781 tggcacgcat cgagcagttt cttgatgctc tgtggctgga aaaaaatctg gctgaaaata
    24841 cgttgaacgc ttaccgtcgc gatctgtcaa tgatggtgga gtggttgcat caccgcgggt
    24901 tgacgctggc gacggcgcaa agtgacgatt tgcaggcatt actggcagaa cggctggagg
    24961 gcgggtataa agccaccagc tcagcgcgtt tgctgagtgc agtgcgccga ttgttccagt
    25021 atctttatcg cgaaaagttt cgtgaagacg atcccagtgc gcatctcgct tcaccgaaat
    25081 tgccccagcg tttgccaaaa gatttaagcg aagcgcaggt cgaacgttta ttacaggcac
    25141 cattaattga tcagccactg gagctacgcg ataaagccat gcttgaagtg ttgtatgcta
    25201 ccgggctgcg tgtctctgaa ctggtcggac tgacaatgag tgatatcagc ctgcgtcagg
    25261 gcgtagtgcg ggtcattggt aaaggcaaca aagagcgcct ggtaccgtta ggtgaagagg
    25321 cggtttactg gctggaaacc tatctggaac atgggcgtcc gtggctgctg aatggtgtgt
    25381 caattgacgt gttgtttccc agccagcgtg cgcagcagat gacgcgacag accttctggc
    25441 accgcattaa acattatgct gtgctggcgg gtatcgacag cgaaaagctg tcaccgcatg
    25501 tattgcgtca cgcttttgcc acccatttat taaatcatgg tgcggattta cgcgtggtgc
    25561 agatgctact gggccacagc gacctctcca ccacgcaaat ttatacgcat gtcgctaccg
    25621 agcgtctgcg gcaacttcat caacagcatc acccgcgggc gtgatgtctg aaaagaacgg
    25681 gaagatttat gaagaaaggt tttatgttgt ttactttgtt agcggcgttt tcaggttttg
    25741 ctcaggctga tgacgcggca attcaacaaa cgttagccaa aatgggcatc aaaagcagcg
    25801 atattcagcc cgcgcccgta gctggcatga agacagttct gactaacagc ggcgtgttgt
    25861 acatcaccga tgatggtaaa catatcattc aggggccaat gtatgacgtt agcggcacgg
    25921 ctccggtcaa tgtcaccaat aagatgctgt taaagcagtt gaatgcgctt gaaaaagaga
    25981 tgatcgttta taaagcgccg caggaaaaac acgtcatcac cgtgtttact gatattacct
    26041 gtggttactg ccacaaactg catgagcaaa tggcagacta taacgcgctg gggatcaccg
    26101 tgcgttatct tgctttcccg cgccaggggc tggacagcga tgcagagaaa gaaatgaaag
    26161 ctatctggtg tgcgaaagat aaaaacaaag cgtttgatga tgtgatggca ggtaaaagcg
    26221 tcgcaccagc cagctgcgac gtggatattg ccgaccatta cgcacttggc gtccagcttg
    26281 gcgttagcgg tactccggca gttgtgctga gcaatggcac acttgttccg ggttaccagc
    26341 cgccgaaaga gatgaaagaa ttcctcgacg aacaccaaaa aatgaccagc ggtaaataat
    26401 tcgcgtgaaa caacagatac aacttcgtcg ccgtgaagtc gatgaaacgg cagacttgcc
    26461 cgctgaattg cctcccttgc tgcgccgttt atacgccagc cggggcgtgc gcagtgcgca
    26521 agaactggaa cgcagtgtta aaggtatgtt gccctggcag caactgagcg gcgtcgaaaa
    26581 ggccgttgag atcctttaca acgcttttcg cgaaggaacg cggattattg tggtcggcga
    26641 ttttgacgcc gacggcgcga ccagca
//
LOCUS       DAEVZA010000063        26422 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEVZA010000063 DAEVZA010000000
VERSION     DAEVZA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.063"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1276
                     /gene="mdtC"
                     /locus_tag="LAF92_002636"
     CDS             <1..1276
                     /gene="mdtC"
                     /locus_tag="LAF92_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBI9791624.1"
                     /translation="SRVNSGMMFITLKPRDERSETAQQIIDRLRVKLAKEPGANLFLM
                     AVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLATLPELADVNSDQQDNGAE
                     MNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMNQYKVVMEVDPRYTQDIS
                     ALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAASTISFNLPTGKSLSDASA
                     AIDRAMTQLGVPTTVRGSFAGTAQVFQETMNSQVILIIAAIATVYIVLGILYESYVHP
                     LTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVKKNAIMMVDFALEAQRHG
                     NLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGSELRQPLGITIVGGLVMS
                     QLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            1277..2692
                     /locus_tag="LAF92_002637"
     CDS             1277..2692
                     /locus_tag="LAF92_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9791625.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            2689..4092
                     /gene="baeS"
                     /locus_tag="LAF92_002638"
     CDS             2689..4092
                     /gene="baeS"
                     /locus_tag="LAF92_002638"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBI9791626.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            4089..4811
                     /gene="baeR"
                     /locus_tag="LAF92_002639"
     CDS             4089..4811
                     /gene="baeR"
                     /locus_tag="LAF92_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBI9791627.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            5002..5334
                     /locus_tag="LAF92_002640"
     CDS             5002..5334
                     /locus_tag="LAF92_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBI9791628.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            5543..5839
                     /locus_tag="LAF92_002641"
     CDS             5543..5839
                     /locus_tag="LAF92_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HBI9791629.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            5841..6137
                     /locus_tag="LAF92_002642"
     CDS             5841..6137
                     /locus_tag="LAF92_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBI9791630.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            6240..7601
                     /gene="yegQ"
                     /locus_tag="LAF92_002643"
     CDS             6240..7601
                     /gene="yegQ"
                     /locus_tag="LAF92_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBI9791631.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(7931..8248)
                     /locus_tag="LAF92_002644"
     CDS             complement(7931..8248)
                     /locus_tag="LAF92_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791632.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            8663..9562
                     /gene="yegS"
                     /locus_tag="LAF92_002645"
     CDS             8663..9562
                     /gene="yegS"
                     /locus_tag="LAF92_002645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBI9791633.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(9644..10417)
                     /locus_tag="LAF92_002646"
     CDS             complement(9644..10417)
                     /locus_tag="LAF92_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI9791634.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(10523..11563)
                     /gene="gatD"
                     /locus_tag="LAF92_002647"
     CDS             complement(10523..11563)
                     /gene="gatD"
                     /locus_tag="LAF92_002647"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBI9791635.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPDDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(11611..12966)
                     /locus_tag="LAF92_002648"
     CDS             complement(11611..12966)
                     /locus_tag="LAF92_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HBI9791636.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(12970..13254)
                     /gene="gatB"
                     /locus_tag="LAF92_002649"
     CDS             complement(12970..13254)
                     /gene="gatB"
                     /locus_tag="LAF92_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBI9791637.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(13285..13737)
                     /gene="gatA"
                     /locus_tag="LAF92_002650"
     CDS             complement(13285..13737)
                     /gene="gatA"
                     /locus_tag="LAF92_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBI9791638.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(13747..15009)
                     /gene="gatZ"
                     /locus_tag="LAF92_002651"
     CDS             complement(13747..15009)
                     /gene="gatZ"
                     /locus_tag="LAF92_002651"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBI9791639.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADRVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(15038..15892)
                     /gene="gatY"
                     /locus_tag="LAF92_002652"
     CDS             complement(15038..15892)
                     /gene="gatY"
                     /locus_tag="LAF92_002652"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBI9791640.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(16202..17254)
                     /gene="fbaB"
                     /locus_tag="LAF92_002653"
     CDS             complement(16202..17254)
                     /gene="fbaB"
                     /locus_tag="LAF92_002653"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBI9791641.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            17511..18788
                     /locus_tag="LAF92_002654"
     CDS             17511..18788
                     /locus_tag="LAF92_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HBI9791642.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18785..19789
                     /locus_tag="LAF92_002655"
     CDS             18785..19789
                     /locus_tag="LAF92_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBI9791643.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            19786..20751
                     /locus_tag="LAF92_002656"
     CDS             19786..20751
                     /locus_tag="LAF92_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBI9791644.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(20725..21471)
                     /locus_tag="LAF92_002657"
     CDS             complement(20725..21471)
                     /locus_tag="LAF92_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI9791645.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(21523..22341)
                     /locus_tag="LAF92_002658"
     CDS             complement(21523..22341)
                     /locus_tag="LAF92_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBI9791646.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YFQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(22406..23206)
                     /gene="thiD"
                     /locus_tag="LAF92_002659"
     CDS             complement(22406..23206)
                     /gene="thiD"
                     /locus_tag="LAF92_002659"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBI9791647.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(23203..23991)
                     /gene="thiM"
                     /locus_tag="LAF92_002660"
     CDS             complement(23203..23991)
                     /gene="thiM"
                     /locus_tag="LAF92_002660"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBI9791648.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(24026..24122)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(24214..24486)
                     /gene="rcnR"
                     /locus_tag="LAF92_002661"
     CDS             complement(24214..24486)
                     /gene="rcnR"
                     /locus_tag="LAF92_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBI9791649.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            24607..25431
                     /gene="rcnA"
                     /locus_tag="LAF92_002662"
     CDS             24607..25431
                     /gene="rcnA"
                     /locus_tag="LAF92_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBI9791650.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHAHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            25650..25988
                     /gene="rcnB"
                     /locus_tag="LAF92_002663"
     CDS             25650..25988
                     /gene="rcnB"
                     /locus_tag="LAF92_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBI9791651.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(26070..>26422)
                     /locus_tag="LAF92_002664"
     CDS             complement(26070..>26422)
                     /locus_tag="LAF92_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9791652.1"
                     /translation="IILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGV
                     GFILESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKIKS
                     GALEATALINVKYD"
BASE COUNT         6428 a         6809 c         6531 g         6654 t
ORIGIN      
        1 ttcgcgagtg aacagcggga tgatgtttat caccctcaag ccacgcgacg aacgcagcga
       61 aacggcgcag caaattatcg accgtctgcg cgtaaaactg gcgaaagaac cgggggcgaa
      121 tctgttcctg atggcggtac aggatattcg cgttggtggg cgtcagtcga acgccagcta
      181 ccagtacacg ttgttatccg acgacctggc ggcactgcgc gaatgggagc cgaaaatccg
      241 caaaaaactg gcgacgttgc cggaactggc ggacgtgaac tccgatcagc aggataacgg
      301 cgcggagatg aatctggttt acgaccgcga caccatggca cggctgggaa tcgacgtaca
      361 agccgccaac agcctgttaa ataacgcctt cggtcagcgg caaatctcga ccatttacca
      421 gccgatgaac cagtataaag tggtgatgga agtggatccg cgctataccc aggacatcag
      481 cgcgctggaa aaaatgttcg ttatcaataa cgaaggaaaa gcgatcccgc tgtcgtattt
      541 cgctaaatgg caaccggcga atgccccact atcggtgaat catcagggat tatcggcggc
      601 ctcgaccatt tcgtttaacc tgccgaccgg aaaatcgctc tcggacgcca gtgcggcgat
      661 cgatcgcgca atgacccagc ttggtgtgcc tacgacggtg cgcggcagtt ttgccggcac
      721 ggcgcaggtg ttccaggaga cgatgaactc gcaggtgatc ctgattatcg ccgccatcgc
      781 cacggtgtat atcgtgctgg gtatccttta cgagagttac gtacatccgc tgacgattct
      841 ctccaccctg ccctcggcgg gcgttggagc gctgttggcg ctggagctgt tcaatgcccc
      901 gttcagccta atcgccctga tagggatcat gctattaatc ggcatcgtga agaaaaacgc
      961 cattatgatg gtcgattttg cgcttgaagc ccaacggcac ggtaacctga cgccgcagga
     1021 agctattttc caggcctgtc tgctgcgttt tcgcccgatt atgatgacta ccctggcggc
     1081 gctgtttggt gcgctgccgc tggtattgtc gggcggcgac ggctcggagc tgcggcaacc
     1141 cctggggatc accattgtcg gcggactggt aatgagccag ctccttacgc tgtataccac
     1201 gccggtggtg tatctctttt tcgaccgtct gcggctgcgt ttttcgcgta aacctaaaca
     1261 aacggtaacc gagtaaatga cagatcttcc cgacagcacc cgttggcaat tgtggattgt
     1321 ggctttcggc ttctttatgc agtcgctgga caccaccatc gtaaacaccg cccttccctc
     1381 aatggcgcaa agcctcgggg aaagtccgtt gcatatgcac atggtcattg tctcttatgt
     1441 gctgaccgtg gcggtgatgc tgcccgccag cggctggctg gcggacaaag tcggcgtgcg
     1501 caatattttc tttaccgcca tcgtgctgtt tactctcggt tcactgtttt gcgcgctttc
     1561 cggcacgctg aacgaactgt tgctggcacg cgcgttacag ggcgttggcg gcgcgatgat
     1621 ggtgccggtc ggcagattga cggtgatgaa aatcgtaccg cgcgagcaat atatggcggc
     1681 gatgaccttt gtcacgttac ccggtcaggt cggtccgctg ctcggtccgg cgctcggcgg
     1741 tctgctggtg gagtacgcat cgtggcactg gatctttttg atcaacattc cggtggggat
     1801 tatcggtgcg atcgccacat tgctgttaat gccaaactac accatgcaga cgcggcgctt
     1861 tgatctctcc ggatttttat tgctggcggt tggcatggcg gtattaaccc tggcgctgga
     1921 cggcagtaaa ggtacaggtt tatcgccgct gacgattgca ggcctggtcg cagttggcgt
     1981 ggtggcactg gtgctttatc tgctgcacgc cagaaataac aaccgtgccc tgttcagtct
     2041 gaaactgttc cgtactcgta ccttttcgct gggcctggcg gggagctttg ccggacgtat
     2101 tggcagtggc atgttgccct ttatgacacc ggttttcctg caaattggcc tcggtttctc
     2161 gccgtttcat gccggactga tgatgatccc gatggtgctt ggcagcatgg gaatgaagcg
     2221 aattgtggta caggtggtga atcgctttgg ttatcgtcgg gtactggtag cgaccacgct
     2281 gggtctgtcg ctggtcaccc tgttgtttat gactaccgcc ctgctgggct ggtactacgt
     2341 tttgccgttc gtcctgtttt tacaagggat ggtcaactcg acgcgtttct cctccatgaa
     2401 caccctgacg ctgaaagatc tcccggacaa tctggcgagc agcggcaaca gcctgctgtc
     2461 gatgattatg caattgtcga tgagtatcgg cgtcactatc gccgggctgt tgctgggact
     2521 ttttggttca cagcatgtca gcgtcgacag cggcaccaca caaaccgtct ttatgtacac
     2581 ctggcttagc atggcgttga tcatcgccct tccggcgttc atctttgcca gagtgccgaa
     2641 cgatacgcat caaaatgtag ctatttcgcg gcgaaaaagg agcgcgcaat gaagttctgg
     2701 cgacccggta ttaccggcaa actgtttctg gcgattttcg ccacctgcat tgtcttgctg
     2761 atcagtatgc actgggcggt gcgtatcagt tttgagcgtg gctttattga ttacatcaag
     2821 catggtaatg aacagcgatt acaactgtta agtgatgcgc ttggcgagca gtatgcgcag
     2881 catggcaact ggcgcttcct gcgcaacaat gatcgctttg tctttcagat cctgcgttca
     2941 tttgaacacg ataattcgga agataaaccc ggcccgggta tgccaccgca cggctggcgt
     3001 acccagttct gggtggttga tcaaaacaac aaagtgctgg ttggtccgcg agcgccgatt
     3061 ccacctgacg gtacacggcg acccattctg gtcaacggtg cggaagttgg cgcggtgatc
     3121 gcctcccccg ttgagcggtt aacgcgcaat actgatatca atttcgataa acaacagcgg
     3181 caaaccagct ggttgattgt cgccctggca acgttactcg cggcacttgc cacttttctg
     3241 ctggcgcgcg gtttactggc accggtaaaa cgacttgtcg atggcacgca caaactggcg
     3301 gcgggcgatt tcactacccg cgtaacgccc accagtgaag atgaactggg caaactggcg
     3361 caagacttca accagcttgc cagcacactg gagaaaaacc agcaaatgcg gcgcgatttt
     3421 atggccgata tttctcacga actgcgtacg ccattagcgg tgctgcgcgg tgaactggaa
     3481 gccattcagg atggcgtgcg taaattcacg ccggagacgg tggcgtcttt acaggcggag
     3541 gtcggtacac tgaccaaact ggttgacgat cttcatcagt tgtcgatgtc tgatgaaggc
     3601 gctctcgcct atcaaaaagc accggtagat ttgatcccac tgctggaagt ggcgggcggc
     3661 gcatttcgcg aacgatttgc cagccgtggc ctgaaactgc aattttccct gccagacagt
     3721 attaccgtat ttggcgatcg cgaccgttta atgcagttat tcaataactt acttgaaaac
     3781 agcctgcgct acactgacag cggcggcagc ctgaaaatct ctgccgagca gcacgacaaa
     3841 acggtgcgcc tgacctttgc cgacagtgcg ccaggtgtca gtgacgatca gctacaaaaa
     3901 ttgtttgaac gtttttatcg caccgaaggt tcccgcaacc gcgccagcgg cggttccggg
     3961 ctggggctgg cgatttgcct gaacattgtt gaagcacata atggccgcat tattgctgcc
     4021 cattcgcctt ttggcggggt aagcattaca gtagagttac cgctggaacg ggatttacag
     4081 agagaagtat gaccgagtta ccaatcgacg aaaacacacc gcgtattttg atcgtggaag
     4141 atgaaccgaa gctgggacag ttgctcattg attatctgcg tgctgcgagc tatgcgccga
     4201 cgcttatcag ccacggcgat caggtactgc cgtatgtgcg ccagacacca ccggatctga
     4261 tcctgttaga tctgatgctc cctggcaccg atggcctgac gctgtgccgg gaaattcgtc
     4321 gtttttctga cattccgatc gtgatggtga cggcaaaaat cgaagagatc gatcgcctgc
     4381 tggggctgga gattggcgca gatgattata tctgtaagcc gtacagccca cgggaagtgg
     4441 tagcgcgcgt caaaaccatt ttgcgccgtt gcaaaccgca gcgcgagttg cagcaacagg
     4501 atgctgaaag cccgttgatt atcgacgaag gtcgttttca ggcttcatgg cgcggtaaaa
     4561 tgcttgacct gacgcctgcg gaatttcgtc tgctgaaaac gctctctcat gaaccaggaa
     4621 aagtgttctc ccgcgagcat ttgctcaatc atctttatga cgactaccgc gtagtaaccg
     4681 accgcaccat cgacagccac attaaaaacc tgcgccgcaa gctggaatct ctcgacgccg
     4741 aacagtcatt tatccgcgcc gtttacggcg ttggttaccg ctgggaagcc gacgcctgcc
     4801 gcatcgttta gttttagcga catgactttg ttagccggag atggcgttca gaccagatcc
     4861 ggcaacatta tcccacgcat ggtcagcaaa ctgacattat ctctccgccc tggcaggcat
     4921 cagaaaatcg ctcatacttt aatcggtaaa cagcaccttt agatgctgtt ttcgatacgc
     4981 aatttcaatc aaggagtcat tatggctggt tggtttgaac tcagtaagag cagtgataat
     5041 cagttccggt ttgtgctaaa agcgggcaat ggtgagacta tcctcaccag cgagctgtat
     5101 acctcaaaag cctctgcgga aaagggcatc gcgtcggtgc gtagcaacag cccgcaagaa
     5161 gaacgctatg agaaaaaaac ggcaagtaac ggcaaattct atttcaatct gaaagccgct
     5221 aatcatcaaa ttatcggctc cagccagatg tacgccaccg cgcaatctcg tgaaaccgga
     5281 attgcctccg ttaaagccaa tggcacaagc cagacggtga aagacaatac gtaattacga
     5341 tgccgggcgt gggttgttgg cgtccggctt tatatccaaa taacgcttag ttattaataa
     5401 ttaagagtaa ccgccgccat taaaatagtg ggcactgacg agcactcgtt gtgctttctt
     5461 tgtttaccat cccatactta tcctcctgaa catgttcatg tgccaaaata tcttctaata
     5521 ctttcgtgac gctatccggt ttatgacata cactgatgta agatttttac ttacaggcgg
     5581 gcgtaaaatg gccagaacga tgacggttga tctcggcgat gagttgcgtg aatttatcga
     5641 gtccttaatt gaatccggcg actatcgcac ccaaagcgaa gtgatccgtg agtcactgcg
     5701 attacttcga gaaaagcaag cagagtctcg cctgcaagcg ttacgtgaca tgctcgcaga
     5761 agggttgagt agtggtgaag ctcagccgtg ggaaaaggac gcgttcttac ggaaggtgaa
     5821 agcagggata aggaaataat gtgcgtatta ttaaactcat gccaaaagcc aatgaggatt
     5881 tagaaggcat ctggtattac agctatcacc atttcggcga accacaagcc gacagatatg
     5941 tggaacatct ttcagacgtt ctccagattc tgagtaacaa taatataggt acgcccagac
     6001 ctgaattagg cgaaggtatt tttgttcttc cttttgaacg ccacgtcatt tatttcctgc
     6061 aatcaccagg ggaaattatc gtcattcgta tccttaacca aaaccaggac gccaccagac
     6121 atttacactg gagctaaacc cgccgattgc gctacaatgc ccgcccttaa agtgggggca
     6181 ctcccctaac cgcttcatca ggtgaagcgg atctgacctg tcatcagaac gagagaatta
     6241 tgtttaaacc ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg
     6301 cttatggcgc agatgctgtt tatgcgggcc agccgcgtta ttccctgcgt gtgcgcaaca
     6361 acgaattcaa ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa
     6421 agttttatgt cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg
     6481 acctgaaacc ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga
     6541 ttatgctggt gcgtgagcac ttccctgaaa tgccgatcca cctttcggtg caggctaacg
     6601 ccgtgaactg ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct
     6661 ctcgcgagct gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga
     6721 tcgagatctt cgttcacggc gcgctgtgca tggcctactc cggtcgctgc ctgctctctg
     6781 gctatatcaa caagcgcgac ccgaaccagg gcacctgcac caacgcctgc cgctgggagt
     6841 acaacgtcca ggaagggaaa gaagatgatg ttggcaacat cgtacacaag tacgagccga
     6901 ttccggtgca aaatgttgag ccgacgctgg gtatcggcgc accaaccgac aaagtgttta
     6961 tgatcgaaga ggcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca
     7021 cttacatcat gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa
     7081 tgggcgtgca ttcgctgaaa atcgaaggtc gtaccaaatc tttctactat tgtgcacgca
     7141 ccgcacaggt ttaccgcaaa gctatcgatg acgccgctgc gggaaaaccg ttcgatacca
     7201 gcctgctgga aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc
     7261 gtcatactca cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc
     7321 agtttgttgg tgagtttacc ggtgagcgca agggggacct cgcggcggta gcggtgaaaa
     7381 ataaattctc cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaatttta
     7441 cccttgagca catggaaaac gccaaaggcg aagctatgcc gatagcacca ggcgatggtt
     7501 atactgtgtg gctcccggtc ccgcaggatc ttgagctcaa ttacgcgctg ctgatgcgta
     7561 atttctccgg ggaaaccacg cgtaatcccc acggtaagtg attaatttcg attatttttc
     7621 ccggatggaa aattcttaga aaccgatcac atacagctgt atttattaag gttatcatcc
     7681 gtttcgctga aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc
     7741 tgtgtaatct cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt
     7801 cttccattga ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc
     7861 atcgtgctcg cacaggttcc ggctaagcac aaccagaacg cgcatgtttg acgcttacca
     7921 aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac
     7981 cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg
     8041 catcattttt tactatattt tctgcaccag atattctgcc cctggctaaa gaagcttcgg
     8101 cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa
     8161 atttaccgat acttgtctca tcattatgca cagcacaacc agacataata aaaatactaa
     8221 ttaatgatat agcagctatc tttttcatct caccttcccc cattaaatac caacgacact
     8281 ctctagtgtt taaatataat aatggcatga ttattataat tgaataggat tataataaat
     8341 gttctgtaca acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa
     8401 ctccatgggg gctttaatat atttggaata gtatatttaa gatgccactg tttggttgaa
     8461 tattaggcat atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc
     8521 cattgcaagc tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct
     8581 ccgactttca tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc
     8641 aatcataaag gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg
     8701 caaaagtact gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat
     8761 gacgatccat gtgcgggtca cctgggagaa aggcgatgcc gcacgatatg tagaggaggc
     8821 ccggaagttg ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt
     8881 ttctacggcg ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt
     8941 aggaaccgcc aatgattttg ccaccagtgt agggattcct gaggcactgg ataaggcgct
     9001 gaaactggca attgccggta acgccattgc gatagatatg gcgcaggtca acaaacaaac
     9061 ctgttttatt aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga
     9121 aaaattaaaa gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga
     9181 tactctgcaa ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc
     9241 cctggtcatt ggtattggta acgggcgtca ggccggtggc ggtcagcaat tgtgtccgaa
     9301 cgcgttaatt aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc
     9361 ggctctcgta tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc
     9421 gtcgtggttt gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt
     9481 gagcggacaa aactttcata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc
     9541 agattgtcca ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgaaat
     9601 gccggatgca gtgtaaatac cttatccggc atacagaaca atactacacc gtaattaatt
     9661 ttacccgtgt cttctgcaat gccagtttat ccacctcaga cacagcatca tcggtaataa
     9721 taatgtcaat tttttcgatt ggcagtacct gattaaaacc gcgacggtta aatttcgatg
     9781 aatcgagaac ggcaatcact ttattagcgg cggtgaccat cgccccgcta atcgaataac
     9841 cttcattaaa ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta
     9901 aatcggcatt aatatcctgc aatgaacgct cggcaatgga accatgcatc gagcgcgttt
     9961 tatggcggac cgtgccaccg cagacgacca gagtaatgtc tttattttcg gaaagggcaa
    10021 acgctgccgg gagactgttg gtaatcaccg tgatattttt ggcggtcatt aatccttcgg
    10081 cgataagcat cgtcgtactt ccgctgtcga gaataacggt catcccttcg tggatcattt
    10141 tgactgccgc ctgcgctatt ctgttttttg gcgcgctggc gagctgaaat cgctctttaa
    10201 acccgacttc ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat
    10261 gaaagcgcgt aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt
    10321 ccgaggcagc aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta
    10381 attggatgat cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg
    10441 tggctagtgt aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc
    10501 agcatcacgc tggcccgcgg tttcatggaa tgagcaacac tttgcccggc atggcattac
    10561 gagcgatgtc acgcaccgcc tgggtgaagc tttcaaagct tccacggtga gcgattaatg
    10621 gctccaggct taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg
    10681 gccaagggct ggaatagttc atccaactgc cgataaccgt cagctcttta cgcaatattt
    10741 tgccaaacgt tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt
    10801 gggcatgagg accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct
    10861 cgaggataag ctgattaaaa cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca
    10921 tttcgctact gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg
    10981 agctaatgtc gatagccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca
    11041 gcaggccaat ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta
    11101 aatgaaaagc atgcagacca acggtaatcg gctcaataaa agccccatcc tcaataggca
    11161 tatccgtggg tagagcaaag acattttttc gcttaacgac aatatattca gcaaatccac
    11221 catcacgccg cgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcagac
    11281 actctggaca agtaaaacag ggtaataacg gcacacaggc aaccgcatcg tcagggtgta
    11341 aatcatcaac accggatccc accgcatcaa tatagccgct aaattcatgg cctaacgtta
    11401 ttggataata atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg
    11461 agctggcaat ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttctg
    11521 caacgcgcac gataccatca gtatcattca ccactgattt cataaaaact cctgattgtt
    11581 aagggggata acctccccct caggtaaaaa ttattctgcg agaacgactt tctcttgttt
    11641 aataaagcca cgcgctctac gccaggtcat gaaaataccg gtcagataaa ttgcaccgat
    11701 aataatgaaa ccgggaatat tttgcgggga gaaaacctga atcagtaacc aggtaatggg
    11761 agaaccgccc tgatccattg aagccaccat acccccggct tttaacgccc cagcattagc
    11821 cgccagttgg gtgtgcaaac caatagtttg cgtcgcgatc cacagggtga tgctcataat
    11881 gatgacaccc gagattaagg tgcggaacag atttccacga tgcacggcga ccgccatcgc
    11941 cacgaagaag ccgatggtgg caagatcgcc aaacggcagc acctgattac ccggcacaca
    12001 aacagcaatt aaaatggtga gtgggataaa aatcaggctt gccgatacca ccgccgtatg
    12061 tcccagcagc aacgccggat caaggccaat caggaactcc tgaccgccga acttcgcctg
    12121 taaacgacta cgagcctgct tagcgatggg cgttaaacca tccatgatgg gtttaatcac
    12181 ccgtggcatt agcagcatca ctgccgccgt ttttaccgcc agctgcaata cacctttgac
    12241 atcgtaaccc gcgaggatgc cgataatcag ccccatcaca aaacccacgg tgacaggctc
    12301 gccaaatgga ccaaatttgc gctgaatatc gtcggcgcta aatttaattc ggttaacgcc
    12361 tgggattttc tcgatgatag catcgaccag caccgcaatc ggccccatat acgccgacgt
    12421 accgtgcgga atagcaatac cttccagctc aaagaaattt cgggtatcgc gggcaaacca
    12481 gtcgccgagc ttataaacaa acgccgcgtg aattaccaca cctgccatcc ctatcatcca
    12541 tgaaccggtt gccagatgca gcaacgcgcc ggtgaaggtc atatgccaga tattccagat
    12601 atcaacattt accacccgcg tcatacgggt cagtaacatc gccacgttaa ccaatatcgc
    12661 aatcggaatc gccaccagcg caatttgcga cgcccaggtc attggtgaag agcccggcca
    12721 gccgacatcg accacatgca gattcaggtc gaaattttcc gccatcgctt tcgccgccgg
    12781 accaatggaa tccagcatta agccaatcac aaggccaatg ccaacaaagc caatcccgat
    12841 atgcagaccc gctttaaagc aatcgcctgc cttcatgcct aatattttag aaaaaataat
    12901 gatgacaatc ggaagcatca ccgtagggcc gaggtcgaga atataacgca tgacttctga
    12961 aaacataggt cacccctgta agatagtcag aattttattt tgtaatgctt cgataccgac
    13021 accagaaata aaaggcatgc cgtgaactaa cggaatatcg ccaaaactac gatccacttt
    13081 ggcagtggtg catatcaaat gcacaccatc catataggtt tctatttcat taacccgaca
    13141 ctggattaat tcaacaggaa tattatgatt ctgacacaac tctttaattt cttccgccgc
    13201 catcgtagag gtcgcaaccg cgcctccgca agcgacaata atcttgcgtt tcataaatag
    13261 tcctttttat tgttacagag agatttattc gtctgaatct aaaacatact ttgtgaagta
    13321 ttcctttaac tgggtttcag gaagagtgat tagtgtctcg acgatatcgg gctgttgtaa
    13381 cttgccaaat aaacagcgta aaagtttcaa ttgctgctgc ggattttcca caatcaacgc
    13441 aataaccaac gatacctcca cgtcgttatc atcatccgct tgctgaaaat gaactttatt
    13501 tgttggcctt aacagataaa tggctgacga cttagcatga atcgcctcac aatgcggtat
    13561 tgcaatggcg tgctgctcaa gcattatccc ggtagggaat tctgcttctc tggcaattaa
    13621 tgcctgtggc caggtatcat gaaccacacc tttggcgagc atctcattac cgatatgggt
    13681 taaaacttcg ctacgatcga caaaagaaat tccgctacga acaaacaggt tagtcatata
    13741 ccgtccttat tccgcacagc cgtagcgata ggcgcgcaaa acatcataaa ttttatccat
    13801 aatcagctga tgcggtattg ctgataattc cccggactga atgcgttcaa attgtttggg
    13861 aagatactga ctaatcatgc ccagtgggat gtccacgcct tgcagattca ccatcatcgt
    13921 ttcgacgcta tttttaatcc gactatgcgg ccagtaataa cgaatacgat ccgacaggct
    13981 gtaacgaata tccagtaatg aatcgttaaa acccgtacga taatattttt tccagtattg
    14041 cggttcgtcg agcatcactt cttcaattac cgccaggcaa ccgctgcgat tttcgggggc
    14101 gataagttcc tgctcaattt gtgccagtgc aaatatcgcc tcgcgtaaag caaaggttaa
    14161 tgcgggaccg actttcaata ttgcaaagtg atcgcggact aattcccaat aagccgtccg
    14221 ggtctggtaa tcggtagaat gtgcttcata aaccattcgg gtattttcta tccattgcgc
    14281 cagcgcctgc gcttcctgcg gctgataatg gataatattg ctgtgatcaa attccacacc
    14341 cggctgcacc acgatggcaa tcacgcgtgt taacgcctct gtcagcccac gggcaataaa
    14401 ggccttttga tgcgtacgta aagtattggc ggcatcttca acatgggtga tgtgtactga
    14461 ctgaatggcg ctggcctcac cgcccggaac cggaacttcg gtgccaatga catagctcag
    14521 ttgctcacgc tggcaatctg tcgccacact ttccgcagca aagcaaagca cagcagctcg
    14581 ttccgcaacc gtttctggtg ctaacggtat gggatccccc gcgcaggaca ttgacgcatc
    14641 aagatgaatt ttactgaagc cagcacgaac atatgccttt accagctcga cggatttttc
    14701 catcgccgca tccacatttt cttgctgcca gcagtttggc cccagatggt caccgccgag
    14761 aataatgcgc tcgcgcgcaa atccgactct atcggcaatc gtaaaaacaa attcgcgaaa
    14821 gtctgccggt gtcattccgg tataaccgcc aaattgattg acctggtttg acgttgcttc
    14881 aatcagcact ttgcgcgtgc tgttgcgatc aaatgccagc gccgcttcga taaccaacgg
    14941 atgggcagaa cagactgaac atatgccgat atgttcacca gctttatgcc gggcaattaa
    15001 cgttttcata ttttttcctt gttaaatggc aggtgcgtta tgccctgccc tcgcagccac
    15061 aatcggcaat cactttgctc accacatcgc gcattgcgga tttagccgac tgcaaataat
    15121 cccggggatc ggtcgcttcg gggtgctcgg tcaggtaatt ttttaacgcc tgcgagaagg
    15181 catttttcag ctccgttgca acgttgattt tgcatatccc cagtttgatg gtttgctgaa
    15241 tatccttagt cgataaccct gacgcgccat gcagcaccag cggtaagttc acccactggc
    15301 gaatgttctc cagtcgagaa aaatcaagcg ccggtgcgct ggcatacata ccatgagccg
    15361 tgccgatggc taccgccagg gaatcaattc cggttgcctc ggcaaattca cgcgcctgag
    15421 cggggttggt ataaaaagca tcggcttcat tgacttgcac atcatcttcc tggccgccaa
    15481 gttgccccag ctccgcttcg acgctgacat caaagcgatg gcaaaaatcc accacctctt
    15541 tgacccgtga aatattttgc gcaaaaggca aatgcgaggc gtcaatcatg actgagcgca
    15601 cgccagaacg aaccttctga gcgatatcgt caaatttcgt gtgatggtct agatgaattg
    15661 ccagtggatg gtgatattgc ttcgccatcg cgctgaccag cgccaacaga ttttctgtac
    15721 cagcatgagt aaatgtgcca ggcgttccgg cgatgatgac cggcgcatgc aggttggcag
    15781 cggtttctac caccacttgc atcgtttcga gattgtgaat attgaatgcc ggaaccgcat
    15841 aaccgccgcg ctgtgcgttg ttcagcatct gctttgtcga taccacgtac attttcatat
    15901 cctgtcgttt gttttcgatt tcaaaatata atgaaatgat ttgtttttaa atgtcgagat
    15961 aacgatcaca aaaacgacaa tgtgaaaatt gttcgaggag taaaaggcaa aaaaaaacgg
    16021 cctcccgata gggaagccgt agcaaagtgc gcgtgttttt atgccggatg cggtgtaaac
    16081 atacgtggca gtgctatgta ggcatgataa gacgcttccg cgtcgcatca ggcataggtt
    16141 gccggatgca gtgtaaacgc cttatccgtc ctacggggtg gtgctgttcg tatgaggcgt
    16201 atcaggcaat agtaattttg ctatcgagat aaacgtcctg cacggcatta atcagtttca
    16261 cgccgtcagc catcgatttc ttgaacgcct tacgtccaag aatcagcccc attccgccag
    16321 cgcgtttgtt gataaccgca gtacgcactg catcgctgag gtcagtttca ccgcccgcag
    16381 caccgccgga gtttatcaac ccagcccgac ccatatagca gttagctaac tgataacgca
    16441 ccagatcaat cgggttttcg ctggtcaatt tgctgtaaac acgatcgtcg gtgtaaccgt
    16501 aattaattgc tttatagccg ccgttatttt ccgccatttt ttgtttgacg atatctgcac
    16561 cgatggttgc cgccagatgg tttgcctgac cggtcaggtc ggcggaaaca tggtaatcaa
    16621 cgccatcttt cttaaaggcg gagttacgca aataggccca cagcactgtc accataccca
    16681 gctcgtgcgc acgttcaaaa gccgcagaaa tttcttcaat ctggcgacgt gactcttccg
    16741 agccaaaata gatagtcgca ccaaccgcaa ccgcgcccat gttgaacgcc tgctccacgc
    16801 tggcatacag cgtttgatcg taggtattcg ggtaacttag cgtctcgttg tgattaagtt
    16861 tgacgaggaa tggaatgcga tgcgcataac gccgcgatac cgacgccagc acgccgtaag
    16921 ttgacgccac acagttacag cccgcttcga tcgccagttc aacaatgttt ttcgggtcaa
    16981 agtagagcgg gttagcagca aatgaagctc cggcagagtg ctcaacgccc tggtcaaccg
    17041 gcagaataga aagatatcct gtgccagcca gacgcccggt gttgtacaac gtctgcatat
    17101 tacgtaacac cgctggcggg cgattattgt caatcattac gcggtctacg tagtcatgtc
    17161 cggggagata aagctggtca gaaggaattg tcatacaacg gtgctgtaaa aggttgtcgg
    17221 cgtctttgcc aagcaactgc gcaatatctg tcattactat gctcccgtaa attccgattg
    17281 gatatcggct atggattgtc ctggcccgcc ttttgcgggc aatcataatc ctggtcgtta
    17341 cgggcgagtt tttccatctt ttggatgttt tttcagcgtt ttctgttggc tcgattcatc
    17401 agaaaaaatg ttagcgcggt caagttttca ccgcaaaggt atttaaaagg tattattaag
    17461 tggtattgtc atcgcgtacc ttacattacc tgtcatgaag gaattaaaag atgaaaacaa
    17521 cagcaaagct gtcgttcatg atgtttgttg aatggtttat ctggggcgcg tggtttgtgc
    17581 cattgtggtt gtggttaagt aaaagcggtt ttagtgccgg agaaattggc tggtcgtatg
    17641 cctgtaccgc cattgcggcg atcctgtcgc caattctggt tggctccatc actgaccgct
    17701 ttttctcggc gcaaaaagtg ctggcggtat tgatgttcgc aggcgcgctg ctgatgtatt
    17761 tcgctgcgca acagaccact tttgccgggt tctttccgtt actgctggct tactctctaa
    17821 cctatatgcc gaccattgcg ctgactaaca gcatcgcttt tgccaacgtg ccggatgtgg
    17881 agcgtgattt cccgcgcatt cgtgtgatgg gcactatcgg ctggattgcc tccggtctgg
    17941 catgtggttt cttgccgcaa atactggggt atgccgatat ctcaccgact aacatcccgc
    18001 tgctgattac cgccggaagt tctgctctgc tcggtgtgtt tgcgtttttc ctgcccgaca
    18061 cgccaccaaa aagcaccggc aaaatggata ttaaagtcat gctcggcctg gatgcgctga
    18121 tcctgctgcg cgataaaaac ttcctcgtct ttttcttctg ttcattcctg tttgcgatgc
    18181 cactggcgtt ctattacatc tttgccaacg gttatctgac cgaagtcggc atgaaaaatg
    18241 ccaccggctg gatgacgctc ggccagttct ctgaaatatt ctttatgctg gcattaccgt
    18301 ttttcactaa acgctttggt atcaaaaagg tattattgct tggtctggtc accgctgcga
    18361 tccgctatgg cttctttatt tacggtagtg cggatgaata tttcacctac gcgttactgt
    18421 tcctcggtat tttgcttcac ggcgtaagtt acgattttta ctacgttacc gcttacatct
    18481 atgtcgataa aaaagccccc gtgcatatgc gtaccgccgc gcaggggctg atcacgctct
    18541 gctgccaggg cttcggcagt ttgctcggct atcgtcttgg cggtgtgatg atggaaaaga
    18601 tgttcgctta tcaggaaccg gtaaacggac tgactttcaa ctggtccggg atgtggactt
    18661 ttggcgcggt gatgattgcc attatcgccg tgctgttcat gatttttttc cgcgaatccg
    18721 acaacgaaat tacggctatc aaggtcgatg atcgcgatat tgcgttgaca caaggggaag
    18781 ttaaatgaaa acagaacgta ttctcggtgc tctttatggg caggcgttag gggatgcgat
    18841 ggggatgccc tccgagcttt ggccacgcag ccgcgttaaa gcacactttg gctggattga
    18901 ccgttttctt cctggaccaa aggagaataa cgcggcctgt tattttaacc gcgccgaatt
    18961 caccgacgat acctcgatgg cgctgtgtct ggcggatgcg ttactggaac gtgaaggcaa
    19021 gatcgatccg gatctgattg ggcgtaatat tctcgactgg gcgctgcgtt tcgacgcctt
    19081 taacaaaaac gtactaggtc cgacctcgaa gattgcgctt aacgccattc gcgacggtaa
    19141 acccgttgct gaactggaaa acaacggcgt gaccaacggc gcagcgatgc gcgtctcgcc
    19201 attaggttgt ttgcttccgg cgcgtgatgt tgattccttt attgatgatg tagcgctggc
    19261 gtccagcccg acccataaat ccgatctggc ggttgcaggc gcggtagtca tcgcatgggc
    19321 gatttctcgt gccattgacg gagaaagctg gtcagcgatt gttgattcac tgccttcaat
    19381 tgcgcgacat gcccagcaaa aacgcaccac cacgttcagc gcctcactgg cggcgcggct
    19441 ggagattgcg ctgaaaattg tgcgcaatgc cgacggcacc gaatccgcca gcgaacagct
    19501 ttaccaggtc gttggcgcag gtaccagcac tattgagtcc gttccgtgcg ccattgcgct
    19561 ggttgaactg gcacaaaccg acccgaatcg ctgcgccgtc ctgtgcgcta accttggcgg
    19621 cgacacagac accatcggtg ctatggcgac ggcaatttgc ggcgcgttgc atggcgttaa
    19681 cgctatcgat cctgcgttaa aggcggaact ggatgcggta aatcagcttg atttcaaccg
    19741 ctatgccaca gcgttggcga aatatcgtca acaacgggag gcggtatgag cggcgctcga
    19801 ttacacacgc tgctgcctga attaaccacg cgtcagccgg tgatggtcgt cggcgcggcg
    19861 gtcattgacg tgatcgccga cgcttatgcc ctcccctggc gtgggtgcga tatcgaactg
    19921 aaacagcaga gcgttaacgt tggcggctgc gcactgaata ttgccgtggc gttaaagcgc
    19981 ctcggcatcg aagcgggtaa tgccttgccg ctcggtcagg gcgtgtgggc ggagattatt
    20041 cgcaaccgga tggcaaaaga gggcttaatc agcctgatcg ataacgccga aggtgataac
    20101 ggctggtgtc tggcgctggt tgagccggat ggcgaacgca cttttatgtc attcagcggt
    20161 gtggaaaatc agtggaatcg ccagtggctg gcgcgattaa ccgttgcgcc tggcagcctg
    20221 ctctattttt ccggttatca actggcctcg ccctgcggcg aattgttagt ggaatggctg
    20281 gaagagctgc aagacgtgac gccgtttatc gattttggcc cacgtattgg cgatatcccg
    20341 gatgcattac tggcgcggat catggcctgt cgacctttag tgtcgctcaa tcgtcaagag
    20401 gctgagattg ccgccgaacg ttttgcttta tccgcagaga taacaacact tggcgagcaa
    20461 tggcaggaga aatttgccgc gccgttgatc gttcgtctcg ataaagaagg cgcatggtat
    20521 ttcagcaacg acgcttctgg ctgcattccg gcatttccaa cgcaagttgt agacaccatt
    20581 ggggcgggcg acagtcatgc aggtggcgta cttgccgggc tggcctctgg tctgccactg
    20641 gcggatgccg tattactggg caatgcagtg gcgtcgtggg ttgtcgggca tcggggcggt
    20701 gattgtgcgc caacgcgcga ggaactactc ctcgcacaca aaaacgtata gatcgctgcg
    20761 acagtggcta atgctgtact caataggccg ttgctgttgg tcaagcgcca cttgcttgat
    20821 caccagcacc ggtattttgc tatctaactg aatatgtgac tgaaactcgg catccggcat
    20881 ccgggcgcta acgcgggaac gcgtacgttg tgggtaaatg tgctggctgc ggaaatagtc
    20941 ataaagtgaa atcccgatgg catcaacatc atgaattaaa tgcgccggaa cccacgattc
    21001 ctcaatcgat accgcttctt catcgacata gcgaatacgc ttaagcaaaa agacatcact
    21061 tcctgcttcg accgccagct gctgcgcgac ttcttcggga catttcacga cacgcttgtt
    21121 aacccatagc gtatcgggct ttttcccgcg caataccacc tgctgagaaa aacctcgcgc
    21181 ttctttcaac gagtattcga agatgttgtt gatctgcgtg ccgtaaccgc gcgaacgcgt
    21241 caccacaccc tcttcttcca gcgcctgcat cgccttgcgc accgtaatgc gcgaaacgcc
    21301 ggttaactga cttaaatcac gctcaccggg caaaatattg ccatgctcca gcaccccgct
    21361 gcgcacggca ttttttaccg tttcggcaaa cttaatataa agcggcgtgt tatccgccgc
    21421 taaaatacgc tcgttcagtt gggcaatcag ctgggtatgc gcttgttcca tttgtctttt
    21481 tcctgccggg gagtctggtg ggagtatatc actgcttctt atttaaggcg tttctttaat
    21541 cccatcaacg aatgcctgca actcctccac cgtgccatta aatacattaa aatccaccgg
    21601 accattgatg ccatctacct gtccacggtc ggaatgctgc cagaagcgcc aggccatgcc
    21661 gtcattgtcc ggacgacgtt gaaaatagtg agccacccac cacggatatt cattgaaata
    21721 gcccgccaga ttcgtgtgat aaaaaacggc tcctgagtaa ataatcggct tttttcccgt
    21781 acttttttcg accattttta gccactgact tactcgtttg cgtaattctt ttgccgataa
    21841 ttttccccgt tcttctacgt ccagtaccgc agggaaatcg ccttgtgaga aatccaccgt
    21901 ttgcagaaat aatcgtgcct gaaccggcgc tgctaccgac ggggagaaat aatgatacgc
    21961 cccgcgcagc aggccatttt cgcggcttag ttgccagtta cgcgaaaaat acgggtccac
    22021 cagcttttcg ccttccgtcg ccttaataaa agcaaactgt aagcggatac cgttgtcgcg
    22081 catttttgcc acccgctgcc agtcgatccg ctcctgccag cgagaaacat ctattccgtg
    22141 aatggtgtaa ctggcaggaa tgcgaatagc gaaagattta accgggcgat aaccgtagaa
    22201 atggatgtag tctgtaatct gaactgctgt cgaatagaaa aaattcaccg tctgacgcgg
    22261 ataaattgca acgatggaaa ttagcccaag agcgcataat agagaggtga gtttttttcg
    22321 actggtaatt cttagctgca ttggtttcat ccctgaatgt cagtgccaaa ggctgacaat
    22381 aaccaaagca gctatagtac ggtgctcacc accaggcgtg gaagtggtga accggaccaa
    22441 taccgtgacc aacttccagc gtgtcggcct gggctaatgc tgatgaaagc cagctttttg
    22501 cctcctgtac ggtgtcagcc cagtttgtat ggcgcgggcg tagtgcagcc aacgccgcag
    22561 agagcgtaca accagtgccg tgggtgtttt tggtcataat gcgcggtgcg gtaaaccgtt
    22621 gttcaccctc gcgggtaaac agccagtccg ggctttgctc atcatccaga tgaccacctt
    22681 tcattagcac tgcgccacag cccatcgcca acagcgatcg cccttgttcc agcatttcct
    22741 gttcggtgcg cgcgtgtggc gcgtcgagca aggcggcagc ttcgggcaag tttggcgtta
    22801 ttaatgaaac ctgtggcaat aatcgactgc gcagcgtagc aaccgccgaa ggtgaaagca
    22861 gcgggtcgcc gctttttgcc agcataacgg tgtcgagtac cacgttttgg atctgataac
    22921 gttgcaaccg ttctgccacc gcttcaacaa tatcggtttc cgccaacata ccgattttag
    22981 tggtgtcgat tcgcacatcg ctgaacaccg aatcgagctg ggcggcgaca aaatcaggct
    23041 caatgcgata caccgactgt acgccacggg tattttgcgc caccagtgca gtaataactg
    23101 agcaaccata agcgccaagt gccgagaagg ttttaagatc ggcctgaatc cccgcaccac
    23161 cactcggatc agtaccggca atcgtcagag cgttaattcg tttcatgcct gcacctcctg
    23221 cgtcaattgc cagagcgcat caaggaaatg tggaacaaaa ctgcctggcc cctcgcttct
    23281 ggcgactgcg cgctctccgg cttgtttcat ccagtgacag gcagatgcga cattttccag
    23341 catatcgcct ggtaacgcac agcaggcagc gacaaccgcc gataatgcac agccagttcc
    23401 taccactttg gtcattaacg gatcgccacc gtgaatacca atgatacgat gtccatcggt
    23461 aacataatcc atctcgccag tgaccacgac gattgcgcca gtttcccgtg ccagtgtttg
    23521 tgcagcgggt atcgcgttag ctgcggcgtc agtggtatcc actccccgtc caccattagc
    23581 aatgccagct aatgccatga tttccgaagc attaccacgt atggcggttg gcttatgaga
    23641 cagaagttcc agacaaaaac ggcggcgata atcgagtgca ccaaccgcta ctggatcaag
    23701 cgtccagggt gtttgagagc tttttgcttg ctcaacggcg gcacacatcg actgtgcgcg
    23761 tagctgcgtc agcgtgccaa cgttaatcag caacgcactg gcaatagccg caaactgact
    23821 ggcctcttcg gtttcgataa ccatcgctgg cgatgcaccg agcgccagca aggtattggc
    23881 agtaaaggtt tgcaccacat cgttggtcat gcagtgcaca agaggggaat gttggtgaaa
    23941 aaggtgtaac gcgtgcgcag attgcgctga actcagcagg tcgacttgca tagtttgctc
    24001 ctgccataac gtgaagaagc aatgacctgg tggtccgtga cttccctacg ctggcattat
    24061 ccagatcagg tgatacgggt atttctcagc cttcacgcag aagggcaccc cgagtcgttt
    24121 ggttgcgatg ataaatcgca gaggaggatg gtaatgtcca gcgcacgcgt tgtaaacgag
    24181 gaaaaacggc agttttacaa tcgcgttaat aaattatttg atatatgaat ccagcacctt
    24241 cagaacgaca tccagatctt cttcacgttt tagctcatcc ccctggtgaa cgatgtgttc
    24301 cgtcagatga cctttaatca cttcccgcat cagaccgttt accgcgccac ggatagcagc
    24361 aatctgttgt aaaactgcag cgcattcgtg cggctcgtcg agcattttct tgagcgccac
    24421 gacctggccc tgaatcttac tggcacgcgc tttcagtttc tgtttatcac ggattgtatg
    24481 agacatggca acacctggtt aacaagaata tgaaaaatca tagcactatt aatctactgg
    24541 ggggtagtat caggtactgg gggggagtag aatcagattg ccgaattaat actaagaatt
    24601 attatcatga ccgaatttac aactcttctt cagcaaggaa acgcctggtt cttcatcccc
    24661 agcgccatct tacttggtgc gcttcatggc ctggaaccag ggcactcaaa aacgatgatg
    24721 gcggcgttta tcatcgccat caaaggcacc attaaacaag cggtgatgct cggactggca
    24781 gcaactattt cgcataccgc agtggtctgg ttaattgcct ttggcgggat ggtgatcagc
    24841 aagcgcttta ctgctcaatc agcagaaccg tggctccagt tgatttccgc agtgatcatt
    24901 attggcaccg cgttctggat gttctggcgt acctggcgcg gcgaacgcaa ctggctggag
    24961 aatatgcacg agcatgatca tgcgcatcat caccacgatc acgaagatca ccacgaccat
    25021 ggacatcatc accatcacga acatggcgag tatcaggatg cccatgcacg agcccatgcc
    25081 aatgacatta aacgacgctt tgatggtaga gaggtcacca actggcaaat tttgctattt
    25141 ggcttaaccg gtggccttat cccctgcccg gcagcaatta ccgtgctgtt gatttgcatt
    25201 cagttgaaag ccctgacact gggcgcaaca ctggtcgtca gtttcagcat tggcctggcg
    25261 ttaacgctgg tcaccgtagg cgttggcgca gcaatcagcg ttcagcaggt cgcaaaacgc
    25321 tggagcggat ttaacactct cgctaaacgc gccccctatt tttccagtct gttgattggc
    25381 ttagttggtg tgtatatggg cgtacatggc ttcatgggca taatgcgata actcaatcct
    25441 ttaggcttgc atgttatctt cagcccagga tgattcttaa atcagctatt tccgctgact
    25501 ctctgctcgc taaggatgct tttagggcat cctttcattt acacttttta cgaaatcatg
    25561 ggatcactaa caaaatatcg cttgtcagtt atattgtatg gcaggaaaga tatgcgactg
    25621 atattacaga tccccaaagt ggagagttta tgaccattaa aaataagatg ttgctgggtg
    25681 cgcttttgct ggttaccagt gccgcctggg ccgcaccagc caccgcgggt tcgaccaata
    25741 cctcgggaat ttctaagtat gagttaagca gtttcattgc tgactttaag catttcaaac
    25801 caggcgatac cgtcccagag atgtaccgca ccgatgagta caacattaag cagtggcagt
    25861 tacgtaacct gcccgcgcct gatgccggga cgcactggac ctatatgggt ggcgcgtacg
    25921 tgttgatcag cgacaccgac ggtaaaatca ttaaagccta cgacggtgag attttttatc
    25981 atcgctaaaa aaagccccct catcatgagg gggaaatgca gacaccttgt tattttttat
    26041 tattagccac ttgctcgtct tgcttgttat tagtcgtatt tcacgttgat taatgcggtt
    26101 gcctccagtg cgccagattt aattttattt gtatcgtaga cgtagtaact ggcagttatc
    26161 ggaattgaaa ccgttgtatc tgtaagtttt gacttcgccg caacttcctt taaatttgta
    26221 tatcctttac tgctgttagt gatatttaaa agtgtgactg gcgaaccatt actttcaaga
    26281 ataaatccta cgccattatc ttccgcacct ttcagtattg tgttgctgtt ggttaacaag
    26341 tcagaacttg ataaatacac ctgcattttc cgtgatgttt gattttgttg acctaataaa
    26401 tttttacaac gcagaataat at
//
LOCUS       DAEVZA010000064        26326 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEVZA010000064 DAEVZA010000000
VERSION     DAEVZA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.064"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..666
                     /locus_tag="LAF92_002665"
     CDS             <1..666
                     /locus_tag="LAF92_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HBI9791653.1"
                     /translation="LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGY
                     QLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQ
                     SICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSH
                     GEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVH
                     GNM"
     gene            638..1021
                     /locus_tag="LAF92_002666"
     CDS             638..1021
                     /locus_tag="LAF92_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbC/YhhH family protein"
                     /protein_id="HBI9791654.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1126..1967
                     /locus_tag="LAF92_002667"
                     /pseudo
     CDS             1126..1967
                     /locus_tag="LAF92_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
     gene            1995..2953
                     /locus_tag="LAF92_002668"
                     /pseudo
     CDS             1995..2953
                     /locus_tag="LAF92_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            2965..3426
                     /locus_tag="LAF92_002669"
     CDS             2965..3426
                     /locus_tag="LAF92_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI9791655.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDTKSKLQELKKLKRMDRRKNKENP"
     gene            4133..4471
                     /locus_tag="LAF92_002670"
     CDS             4133..4471
                     /locus_tag="LAF92_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791656.1"
                     /translation="MADKAILWALISASNKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKSLI"
     gene            complement(5029..6165)
                     /locus_tag="LAF92_002671"
     CDS             complement(5029..6165)
                     /locus_tag="LAF92_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI9791657.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(6168..6530)
                     /locus_tag="LAF92_002672"
     CDS             complement(6168..6530)
                     /locus_tag="LAF92_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBI9791658.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            7067..8980
                     /gene="mtlA"
                     /locus_tag="LAF92_002673"
     CDS             7067..8980
                     /gene="mtlA"
                     /locus_tag="LAF92_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBI9791659.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            9210..10358
                     /gene="mtlD"
                     /locus_tag="LAF92_002674"
     CDS             9210..10358
                     /gene="mtlD"
                     /locus_tag="LAF92_002674"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBI9791660.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            10358..10945
                     /gene="mtlR"
                     /locus_tag="LAF92_002675"
     CDS             10358..10945
                     /gene="mtlR"
                     /locus_tag="LAF92_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBI9791661.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(10957..11166)
                     /locus_tag="LAF92_002676"
     CDS             complement(10957..11166)
                     /locus_tag="LAF92_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791662.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            11451..11813
                     /locus_tag="LAF92_002677"
     CDS             11451..11813
                     /locus_tag="LAF92_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBI9791663.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            12357..13040
                     /locus_tag="LAF92_002678"
     CDS             12357..13040
                     /locus_tag="LAF92_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBI9791664.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            13084..17934
                     /gene="ehaG"
                     /locus_tag="LAF92_002679"
     CDS             13084..17934
                     /gene="ehaG"
                     /locus_tag="LAF92_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HBI9791665.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            18303..19958
                     /gene="lldP"
                     /locus_tag="LAF92_002680"
     CDS             18303..19958
                     /gene="lldP"
                     /locus_tag="LAF92_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBI9791666.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            19958..20734
                     /gene="lldR"
                     /locus_tag="LAF92_002681"
     CDS             19958..20734
                     /gene="lldR"
                     /locus_tag="LAF92_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBI9791667.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            20731..21921
                     /gene="lldD"
                     /locus_tag="LAF92_002682"
     CDS             20731..21921
                     /gene="lldD"
                     /locus_tag="LAF92_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBI9791668.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            22107..22580
                     /gene="trmL"
                     /locus_tag="LAF92_002683"
     CDS             22107..22580
                     /gene="trmL"
                     /locus_tag="LAF92_002683"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBI9791669.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(22633..23454)
                     /gene="cysE"
                     /locus_tag="LAF92_002684"
     CDS             complement(22633..23454)
                     /gene="cysE"
                     /locus_tag="LAF92_002684"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBI9791670.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(23534..24553)
                     /gene="gpsA"
                     /locus_tag="LAF92_002685"
     CDS             complement(23534..24553)
                     /gene="gpsA"
                     /locus_tag="LAF92_002685"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBI9791671.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(24553..25020)
                     /gene="secB"
                     /locus_tag="LAF92_002686"
     CDS             complement(24553..25020)
                     /gene="secB"
                     /locus_tag="LAF92_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBI9791672.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(25083..25334)
                     /gene="grxC"
                     /locus_tag="LAF92_002687"
     CDS             complement(25083..25334)
                     /gene="grxC"
                     /locus_tag="LAF92_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBI9791673.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(25475..25906)
                     /locus_tag="LAF92_002688"
     CDS             complement(25475..25906)
                     /locus_tag="LAF92_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBI9791674.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVISRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGLQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            26151..>26326
                     /locus_tag="LAF92_002689"
     CDS             26151..>26326
                     /locus_tag="LAF92_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791675.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDR"
BASE COUNT         7048 a         6281 c         6521 g         6476 t
ORIGIN      
        1 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
       61 gacccgctgc aggggcgata tatcactcag gatccgattg gactgaaggg gggatggaac
      121 ctgtatggat atcaattgaa tccgatatca gacatcgacc ccctgggttt atctatgtgg
      181 gaggatgcaa aatcgggggc atgtactaat ggtctttgcg gcacactatc cgctatgata
      241 ggtccagata aatttgattc tatagatagc accgcatatg acgccttaaa taaaataaat
      301 agccaatcta tttgcgaaga taaagagttc gctggtttaa tatgtaagga taatagtggc
      361 agatatttct caacagcacc taaccgagga gaaagaaaag gatcatatcc attcaatagc
      421 ccttgcccta atggtactga gaaagtatca gcttatcata ctcatggtgc agatagtcat
      481 ggagaatatt gggacgaaat attttcaggt aaagatgaga aaatagttaa aagtaaagat
      541 aacaatatca agtcatttta tttaggtacg cccagtggta attttaaagc aatagataac
      601 cacgggaagg aaataacaaa cagaaaagga ttacctaatg tctgcagagt tcatggtaat
      661 atgtaaaaaa atattgttta ggaactgtgt cattgtatct ttgtttgttt ttacatacaa
      721 cacatgggcg cagtgtaata ataatattaa aataatgcgc aagtatgaaa gtgaaggtaa
      781 atataccgtt agaaatttgg ttaaaaataa agctatagca ttggaattag ctgagatata
      841 tgttaagaat cgttatggac aggatgccgc agaagaagaa aaaccatacg aaatcactga
      901 gttaacaaca agttgggttg ttgaaggtac cattcactca gaccaaattg ctggtggggt
      961 ttttattata gaaataggca aaaatgatgg gagaattctg aattttggcc acggaaaata
     1021 agtatgtaag gataatcaaa catcgtgtgc gctgatggca gagtaggtgg aggactccag
     1081 acagtcaaac gatagaaaaa gatagccttt atggaggttc ctgcaatgtc aaatacatac
     1141 cagaaaagaa aggcaagtaa agagtatggt ttatataatc aatgtaagaa actaaatgat
     1201 gatgaattat ttcgcttact tgatgatcac aattccttga aaaggatttc atctgccaga
     1261 gtattacagt taagaggtgg gcaagacgct gttagattgg caattgagtt ctgctctgat
     1321 aaaaattata tccgtagaga tatcggagca tttatactcg ggcaaataaa aatttgcaaa
     1381 aaatgcgaag ataatgtttt taatattttg aacaatatgg cattgaatga taagagcgct
     1441 tgcgttcgag ctacggcaat cgagtcaacg gctcagcgat gcaagaaaaa ccaaatttat
     1501 tcacctaaaa tagtagaaca atctcaaatt actgcttttg ataaatcgac taatgtcaga
     1561 cgtgctacag catttgctat ttctgttatc aatgataaag caacaattcc actattgatt
     1621 aatctgttaa aagatccaaa tggagatgtc agaaactggg ccgcatttgc aataaatatc
     1681 aataaatatg ataatagtga tattagggat tgttttgtgg agatgcttca ggataaaaat
     1741 gaggaagtcc gtattgaagc aataatcgga ctttcctaca gaaaagataa aagggtttta
     1801 tctgttttat gcgatgagtt aaaaaaaata ctgtttatga tgatatcatt gaagctgcgg
     1861 gtgaattagg tgataaaacg ctacttcctg ttttagatac tatgttgtac aagtttgatg
     1921 acaatgaaat tataacttcc gctattgata agctgaagcg ttcatagcgt gagttgccta
     1981 tgcacagtgg gggattcccg ccggcacggt gcagatgctc gaccggctgg aaagtgaaat
     2041 cctggctgac cgggtgagtg aggaaagccg ccgctggctg gcatcgtgcg gcctgaccgt
     2101 ggagcagatg aaaaaccaga tggacccggt gtacacgccg gcgcgaaaaa tccacctgta
     2161 ccactgcgac catcgcggcc tgccgctggc gctcatcagc acggaagggg caacagcgtg
     2221 gtgcgcagaa tatgatgaat ggggcaacct gctgagtgat gagaacccgc atcatctgca
     2281 gcagctcatt cgcctgccgg gtcagcagta tgatgaggag tccggcctgt attacaaccg
     2341 ccaccgctat tatgacccgc tgcaggggcg gtatatcact caggatccga ttgggctgaa
     2401 ggggggatgg aatttttatc agtatccgtt gaatccggtc ataaatgtag atccgcaagg
     2461 tttggttgat ataaatttat accccgaaag tgatcttatc cattctgtag ctgatgagat
     2521 taatatccca ggcgttttca caatcggggg gcatggtacc cccacatcta ttgaatccgc
     2581 aacgcgcagt atcatgacag ctaaagatct agcatatcta attaaatttg atgggaatta
     2641 taaagatggg atgacagttt ggttattttc ttgtaataca ggtaaaggac aaaattcatt
     2701 tgctagccaa ttagctaaag agttacatac aaatgtaata ggacctgaca cgctatggac
     2761 gtggtggggg cgaggaacta atggtaagtt aaaaatggat acagtgctaa cagcaccaac
     2821 gaaccttaat tcaaataagg atctaatggc tataacaaca aaagaccttg gtaattggat
     2881 aacatatggg ccatctgggc accccatttc taatatgcaa ggtacgccag aaaaacccag
     2941 tgatataaga taggttgtag atgtatgaaa gcatgcttgt tactattttt ttatttttct
     3001 cttattggtc aattgcatgg tgctgatgtg aaaataaaag aaaacgaaag tgtgatggga
     3061 tctacagcaa tgacctatga tttaagtgaa gaaaagctga tgaaactgaa gtataaatca
     3121 caacatggtg attcagaggc atcatttcga ctatatcaat attactgctt tactaaaaat
     3181 aatattgata aacaattgcg attcttggaa agatcagcat ctcaggggaa tgttacagcg
     3241 cagtttaatt atggggtctt tttatcagat acaaatccaa cattatcaga atattataat
     3301 ttgaatagag ctatttattg gatggaattc gctgtaaata acggtaatat tgatacaaag
     3361 agcaaacttc aagagctcaa aaagctaaag cgaatggata gaaggaagaa taaggagaat
     3421 ccgtaaaagc aatagttcat cagactgccg cagcagtagt atgatgatga atcagggctg
     3481 tactataact attatcggta acattaaccg cagcaggggc ggtataacac tcaggctccg
     3541 attgggctgg aaggtaggtg gaatttttat cagtatccgt tgaacccgat ttctggaatc
     3601 gatccccttg ggattagcca cctgtttata cagtataact ttaggtatgc tttcgtgtgt
     3661 ttcagatata ccatgtgatg ataattttta tgatgtgctt agtattccag tcgcttctgg
     3721 taataatatg caatgtaaaa ataatccccg ttgtacacat ttataaatag atggccaata
     3781 cctcaaggcc tatggagttg gaatgttaat gacccgggag caagcaatag aaaacctgac
     3841 ggcatacgat tagtaccgtc agttaataca ggaacttata atcgcaatgg attttcaata
     3901 catagttgtt taaacgcttt tggtccgtct ttaggacctc gattctgttc cgagggttgt
     3961 ataactggtt tatctaatga catgcaaaaa ttaaatgagt taatattttc agagccagat
     4021 agcgcattaa cagttacgga ctaaaaaata tggcaaggtt attaacaatt gtattttttt
     4081 ctctatttct gcgagtgcag ttactgcaag acaatttcag agagagttag atttggcaga
     4141 caaagcaata ttatgggcac taatatcagc cagtaataaa gagggacgaa aagcgtgttc
     4201 actaagctat tttgcctgta aggccgcaga ggctgaactc ggactggcat atatggctgc
     4261 gaatgataac aaggaatttc tcacatcctt atctaatata atgaggtaca aaatagatgc
     4321 tggactttcc gaatcctata catgctattt attaagtaag ggaaagatca taagaccata
     4381 tctgaaaaat ctaaatccac tccaacttgc cgctgattgc attgaaacag taaataaaat
     4441 aaaagataag aataaaaaat cattgatata aattcagtta atatttgtag tgatgataaa
     4501 aatataaaat tgagagttaa tagtaccatt atggccattg atgattctat aaagtgcatc
     4561 gatgaatgat aacttaattt tatttaaaaa tatattaata actcactctc caggagtttt
     4621 tttaacttag ttttataatg aatacgataa ttaaaagtga agaagaaaat agttgtatac
     4681 atatattcca tgaccctaca ttgtgatcta tagattaaat attttttaaa tgtgactgtc
     4741 atccttcaga taataaagcc atcatcgcaa tagatggaaa agcactcctg ctcttttttg
     4801 attagagccg tcgcaggagg aacaactcat atcatcattg cgatctcgac gatgtatagt
     4861 ctcatcctgc ggcagaacaa gacggataaa aaatccaatg agagcacact catccagaac
     4921 ttattaacat aatgaatatt aaagaaaaat catcgcagct aatgaagtaa tatgcaaaaa
     4981 taattagtcc ttacaaggag caaagttcca taatctcccc cctccccctc aatgatccaa
     5041 ataaagataa tgcatccagc tggtcattct tagcagcact ttccgcatca ccgaaacatg
     5101 gctgaaatgg tcggagtaaa ccgccacctg ggcgtagatg ccgtcgggta aggcatcgat
     5161 atcatcgtta gggtccagtt caatggttcc cagcacaccg tccgtgccgg gcacgaccgt
     5221 taatgattgc aataccccct gcgcctgata agaaccgcct ggcacgacag gtaaaatact
     5281 agtcagtttg ccgtgaaaca cctgcccagg tagcgcgtta aacaccactt ccgcatcatc
     5341 gccgggtttc agacgtaaca gcgagttttg ccgaaattgg gcgacaattt gccgtttttg
     5401 ctcggggatg aagaccatca ccggacgcag cggcaaggca gctgcgtatg tacctgggcg
     5461 gatcagtacc tgagtaacgt agccattgct cggcgcgcga atgacagtct gctcaaggtt
     5521 atattttgct tcagtaagtt gcgctcttaa gctcacaatc tgcgattgct cgccgttaac
     5581 catactgtcg agctggctct ggatctgcgc ctgctccgcc accgagcctt tcaccagcgc
     5641 atcctgcgcg aggaaatttt gccgcgcatc gtcgatgtca cgttccgaga acggattcac
     5701 cgccgcctgg ctgcctttca agtaacgttg ataattttta aacagacggt cgcgctccgc
     5761 tgaaacctgg gtggtgttgg cctgcgcttc agtgagctgc gcacgcagcg tctttatatt
     5821 atgcgtcgcc gtcatcaggt cagcctgaag tctgtcaact cgcgcctggt aacgaaccgg
     5881 gtcgagctta aaaagcacct cgcccttttg aataagctga ttattcttgt cagtgacttc
     5941 agtaacaatt cccgtcacct gtggcgtgat ggggatcgct atcactgcct tttgcgcggt
     6001 aaaagtgtaa gggtggttgt agttcatcaa caaaatcaaa ccactcacca gaaacacgcc
     6061 acccagcgcc gccgtcgcca gcgtccactg attcaccgga atacgaaaga ttttaaagac
     6121 cgcccacgcc agcgccacgt aagttaaaac aatcaataga tccataatta gatctccggg
     6181 aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg ggaaattcgg
     6241 tcagaaagtg cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc
     6301 ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa cggccagata
     6361 acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg
     6421 ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc
     6481 accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg
     6541 gattcacgtt tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt
     6601 ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg
     6661 atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg tagatcacat
     6721 caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc
     6781 aaaacaaaaa tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgtctt
     6841 gttgcttttt tacacaagcg ttttgtgatg agtatcacgt caattacctc tctaccccct
     6901 atatttatgt gactgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg
     6961 ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct
     7021 cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat
     7081 taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg
     7141 cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa
     7201 cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg
     7261 ttataccggt ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcactac
     7321 catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc
     7381 gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg
     7441 ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgccattct
     7501 ggcatttctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt
     7561 taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa
     7621 aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca
     7681 gtcccatgaa ctgggtaaat ccatcttctt cctgattgaa gctaacccag gtccaggtat
     7741 gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg
     7801 tgcggcaatc atccacttcc tggggggtat ccacgaaatc tacttcccgt atgtgctgat
     7861 gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac
     7921 tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc
     7981 gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt
     8041 ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat
     8101 tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct
     8161 gtctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga
     8221 cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc
     8281 aggtctgtca cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga
     8341 cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca
     8401 gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct
     8461 cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag
     8521 ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg
     8581 caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg
     8641 ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta
     8701 tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa
     8761 aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga
     8821 cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac
     8881 cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag
     8941 cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccca ccctctccac
     9001 atggagaagg tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc
     9061 acaatttgtt gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc
     9121 gctgcccctc tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc
     9181 gggtaaaaac attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata
     9241 tcggtcgtgg ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg
     9301 atgtcaatca ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg
     9361 ttggtgaaac cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg
     9421 atgatgtcgt tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg
     9481 tgctggaacg tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta
     9541 atgaatcccc gttgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga
     9601 aaggccatgt gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg
     9661 gctttgtcga ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc
     9721 cgctggaagt gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag
     9781 gcgcactgcc gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac
     9841 gtaaactctt caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg
     9901 gtcatcagac cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg
     9961 cgatggaaga aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg
    10021 cggcgtacat ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag
    10081 agcgcgtagg ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat
    10141 tgctcggtac gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg
    10201 caatgcactt ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg
    10261 acaaaggtcc gcaggcggcg ctggcacaga tttccggtct tgatgccaac agcgaggttg
    10321 tatccgaggc ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac
    10381 cctgcgcccg aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt
    10441 tgaaaaccgt gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac
    10501 cgccgtcgag cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga
    10561 cgattacgcg gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga
    10621 tctttctgtg cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga
    10681 agatgcggag ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc
    10741 ctttaccgac gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc
    10801 accgccgcca cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta
    10861 tcaacaggct gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag
    10921 cttaaaaaaa gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg
    10981 ccgataaagc cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg
    11041 tattccggac ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg
    11101 gcttgatgcc atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt
    11161 gcccatgatg atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt
    11221 taggttcccg gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct
    11281 gctgtttatt atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta
    11341 agcatgttaa tgattacgtt atagaactat tgcaatgtca tcagttgtga acaaagcacc
    11401 tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag
    11461 tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg
    11521 ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca
    11581 cgctggaagc agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag
    11641 cacagaagct gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata
    11701 tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg
    11761 gccgtgaaat gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc
    11821 agacgactgg ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg
    11881 actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc gatttaccgc
    11941 tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca ataaaaatat
    12001 atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg gatttaattg
    12061 tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc ccagacatgg
    12121 tttctttcat atttgacatt tattaataat catcattaga atccacgaaa aataatatat
    12181 tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa
    12241 caatatttaa tcgacacaca tcactgtcat taatgtaaag aaacatagct caccaataat
    12301 acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga
    12361 acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat
    12421 gtgactacat tgaagaaggc aagccagaaa gtagtttatt gaaacaacaa gaagaacata
    12481 acaataaaat tgatctactt gaaaaacaac aagcacagct aaagagtcaa ctcgaaacta
    12541 ttcaaaaaca acaaactggc ataataaaca gtacaaagac tttaactcac gtaattaaat
    12601 ccgttaaaga ccaacaaaat acttttattt ttaccgagtt taacccagca aaaacgaagt
    12661 atttcatcct gaacaacggt tcggtggctt tagcgggtcg agtgttatcc attgacgcca
    12721 cagagaatgg tagtgttatt catatctcac tggtcaactt attaagtaca cctatctcaa
    12781 acattggttt taatgcgaca tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc
    12841 ggtggcaaca attacttttc aacacatcaa tgaaatccac attgaaatta ttaccaggtc
    12901 aatggcaaga cattaatttg accctgaagg gtgtatcgcc caataacctg ggatatctga
    12961 agttagccat caacatggaa aatattcagt tcgacaatct tccctctgct gaaaatcggc
    13021 agaaaagaag caaaaaataa atgcagttag tacgcttata acaaggaata gcgagcatca
    13081 ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta tactgttacc
    13141 agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg
    13201 ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac
    13261 ggtcagggtg ttgattacgg tagtggatca gctggcgacg gctgggttgc tataggcaaa
    13321 ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt gggttatgac
    13381 gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc gattggcggt
    13441 gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat agcactgggt
    13501 gcctcttcgt attcattggg ccaatactca atggccctcg gccgttattc aaaagcattg
    13561 ggtaaattgt ctattgctat gggggactct tccaaagcgg aaggagcaaa cgccattgcc
    13621 ctgggaaatg ccactaaagc tactgagatt atgagtattg ctcttggcga caccgccaat
    13681 gcgtcaaaag cgtattcaat ggcgctggga gcaagtagcg tcgcatctga agaaaacgca
    13741 attgccctgg ggcgtagcag tgtagctagc ggtactgaca gcctcgcatt tggcagacaa
    13801 tcacttgcca gcgcagcgaa cgctattgcg ataggtgctg agaccgaagc cgctgaaaat
    13861 gcaactgcta ttggcaataa tgcgaaggca aaaggaacta atagcatggc aatggggttc
    13921 ggaagccttg ccgataaagt caatactatc gcattaggaa atggcagcca ggctctggca
    13981 gataatgcaa tcgccatagg ccagggcaac aaagctgatg gcgtggatgc catcgctctg
    14041 ggtaatggta gccagtcgag aggcttaaac accattgcct taggcacagc cagtaatgca
    14101 actggtgata agagtcttgc gcttggtagt aatagcagtg ccaacggtat taactctgtc
    14161 gcgctgggcg cagattccat tgcggattta gacaataccg tctctgtcgg caatagttca
    14221 ttaaaacgca agatcgttaa tgtgaaaaat ggcgcgatca agtctgacag ttacgatgcc
    14281 attaatggtt cacagcttta tgccattagc gactcggtag caaaaaggct tggaggaggg
    14341 gctgcagtag atgttgatga cggtactgtt acagcaccaa cctacaattt aaaaaatggt
    14401 agcaaaaata acgtaggggc tgcgctcgct gtacttgatg aaaacaccct gcaatgggac
    14461 caaaccaaag gcaaatacag cgctgctcat ggtactagta gcccaactgc cagcgtaatc
    14521 accgatgttg cggatggcac gatttcagcc tccagtaagg atgcggttaa cggttcccaa
    14581 ctgaaagcta ccaatgacga tgtcgaagcc aacaccgcca atatcgctac taataccagc
    14641 aacattgcca cgaatacggc aaatattgcc accaatacca ccaatatcac caacctgacg
    14701 gattccgttg gtgaccttca ggctgatgcc ctgctctgga acgaaactaa aaaggcattc
    14761 agtgcagctc acggccagga taccaccagc aaaatcacca acgttaaaga tgccgacctg
    14821 acggctgaca gcactgatgc tgttaacggc tctcagctga aaaccaccaa cgatgctgtg
    14881 gcgacgaata ccaccaatat cgccaataac acttccaata ttgccactaa caccaccaac
    14941 atctctaacc tgactgagac ggtgactaat cttggtgagg atgcgctgaa atgggataag
    15001 gacaatggtg tattcacggc agctcatggc accgagacca ccagcaaaat caccaacgtt
    15061 aaagatggcg acctgacgac tggcagcacc gatgccgtta acggctctca gctgaaaacc
    15121 accaacgatg ccgtggcgac gaataccacc aatatcgcca ctaacaccac caacatctct
    15181 aatctgactg agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat
    15241 ggtgtcttca ctgcagctca tggcaacaat accgccagca aaatcaccaa tatcctggac
    15301 ggcacagtca ctgcaaccag ttccgatgcc attaacggta gccagcttta tgacttaagc
    15361 agcaatatcg ccacctactt cggcggcaat gcttctgtga atactgacgg tgtgtttacc
    15421 ggtccaacct acaaaatcgg tgaaacaaat tattataacg tcggcgatgc actggctgcg
    15481 attaactcct catttagcac gtctctcggc gatgctctgc tttgggatgc caccgcaggt
    15541 aaattcagtg ccaaacacgg tactaatggt gacgcaagcg tgatcactga tgtcgcagat
    15601 ggtgaaattt cagactccag ttctgacgca gtaaacggct cacaactcca cggcgtgagc
    15661 agttatgttg ttgatgcgct ggggggtggt gccgaagtca atgcagacgg caccatcact
    15721 gcgccgacgt acaccattgc taatgctgat tacgataatg tcggtgatgc cctgaatgct
    15781 atcgatacca ctcttgacga cgctctgctc tgggatgcgg acgccggtga aaatggtgca
    15841 tttagcgccg ctcacggaaa agataaaact gccagtgtaa tcactaacgt cgctaacggt
    15901 gcaatctctg ctgccagcag cgacgcgatt aacggctcac aactctatac caccaataag
    15961 tacatcgctg atgcgctggg tggtgacgca gaagtcaacg ctgacggcac catcaccgca
    16021 ccgacttaca ccattgcgaa cgccgagtac aacaacgtcg gtgacgccct ggatgcgctt
    16081 gatgataacg ccttgctgtg ggatgagact gccaatggcg gtgctggagc ctacaatgcc
    16141 agccatgacg gtaaagccag catcatcact aatgtcgcta atggcagtat tagtgaggac
    16201 agtaccgatg cagtgaacgg ttctcagttg aatgcgacga atatgatgat tgagcagaac
    16261 acccaaatta tcaatcagct cgctggtaac accgacgcaa cctatatcca agaaaacggt
    16321 gcgggtatta actatgtgcg tactaacgac gacggcttag cgttcaacga cgccagcgca
    16381 cagggtgttg gcgctacagc tataggttat aactctgtcg ccaaaggcga tagcagcgta
    16441 gctattggtc agggcagcta cagcgacgtt gatacgggta tcgccctggg tagcagctct
    16501 gtttccagcc gagtgattgc caaaggctcc cgtgacacca gcataacgga aaatggcgtt
    16561 gttattggtt acgacaccac ggatggcgaa ctgctcggtg cattgtctat cggtgatgac
    16621 ggtaaatatc gtcaaatcat caacgtagcc gatggttccg aagcccatga cgccgttacg
    16681 gttcgtcaat tgcagaatgc gattggtgcg gtcgcaacca cgccgactaa atacttccac
    16741 gctaattcaa cggaagaaga ttcactggca gtgggaactg actcgctggc aatgggtgcg
    16801 aaaaccatcg tgaatggcga taaaggtatt ggtatcggtt atggtgccta cgtggacgcg
    16861 aatgcactta acggcattgc cattggtagc aatgcgcaag tcattcatgt caacagtatt
    16921 gcgataggta atggttctac gaccactcgt ggcgctcaaa ccaattatac cgcctacaac
    16981 atggacgcac cgcagaactc tgtcggtgaa ttctcagtcg gtagtgcgga tggtcaacgt
    17041 cagatcacta acgtcgcagc aggttcggct gataccgatg cggtcaacgt gggtcagttg
    17101 aaagtaacgg atgcgcaggt ttcccagaat acccagagca ttactaacct ggataatcgg
    17161 gtaacgaatc ttgattcacg cgtcaccaat atcgaaaacg gtattggcga tatcgtcacc
    17221 accggtagca ccaagtactt caagaccaat accgatggtg tagatgccag cgcgcagggt
    17281 aaagatagcg tcgcgattgg ttctggctcc attgctgccg cggataacag cgtcgcactg
    17341 ggtacagggt ctgtggcaac cgaagaaaat acgatctctg taggctcatc tactaatcaa
    17401 cgtcgtatca ccaacgtagc cgcaggtaaa aacgacaccg atgctgttaa cgtggcacag
    17461 ttgaagtctt ccgaagctgg cggtgtgcgt tacgacacca aagctgatgg ttctatcgac
    17521 tatagcaata tcaccctcgg tggcggcaac ggcggtacga ctcgtatcag caacgtctcc
    17581 gctggcgtca acaacaacga cgcggtgaat tacgcgcagt tgaagcaaag cgtgcaggaa
    17641 acgaagcaat acaccgatca gcgaatggtt gaaatggata acaaactgtc taaaactgaa
    17701 agcaagttga gcggtggtat cgcttctgca atggcaatga ccggtctgcc gcaggcttat
    17761 acaccgggtg ccagcatggc ttctattggt ggcggtactt acaacggtga atcggcagtt
    17821 gctttaggtg tatcgatggt gagcgccaat ggtcgttggg tctacaaatt acaaggtagt
    17881 accaatagcc agggtgaata ctccgccgca ctcggtgccg gtattcagtg gtaatgatcc
    17941 attaagttag tgtgactaag ggcaaacgcc aaattgcctg atgcgttacg cgcatcaggc
    18001 aagataaggc gctccgggtc aacaatctaa gggcaattct ctgatgagga ttgccctttt
    18061 tctttaccag acatctcccc ccacaagaat tggccctacc aattcttcgc ttatctgacc
    18121 tctggttcac aattccccaa ttaaaactca catcaatatt gccaatacat aacatttagt
    18181 taaccattca ttgtcattat ccctacacaa cacaattggc agtgccactt ttacacaacg
    18241 tgtgacaagg agatgagcaa cagactcatt acacgatgtg cgtggactcc aggagacctg
    18301 caatgaatct ctggcaacaa aactacgatc ccgccgggaa tatctggctt tccagtctga
    18361 tagcatcgct tcccatcctg tttttcttct ttgcgctgat taagctcaaa ctgaaaggat
    18421 acgtcgccgc ctcgtggacg gtggcaatcg cccttgccgt ggctttgctg ttctataaaa
    18481 tgccggtcgc taacgcgctg gcctcggtgg tttatggttt cttctacggg ttgtggccca
    18541 tcgcgtggat cattattgca gcggtgttcg tctataagat ctcggtgaaa accgggcagt
    18601 ttgacatcat tcgctcgtct attctttcga taacccctga ccagcgtctg caaatgctga
    18661 tcgtcggttt ctgtttcggc gcgttccttg aaggagccgc aggctttggc gcaccggtag
    18721 caattaccgc cgcattgctg gtcggcctgg gttttaaacc gctgtacgcc gccggactgt
    18781 gcctgattgt taacaccgcg ccagtggcat ttggcgcgat gggcattcca atcctggttg
    18841 ccggacaagt aacaggcatc gacagctttg agattggcca gatggtgggg cggcagctac
    18901 cgtttatgac cattatcgtg ctgttctgga tcatggcgat tatggacggc tggcgcggta
    18961 tcaaggagac gtggcctgcg gtcgtggttg cgggcggctc gtttgccatc gctcagtacc
    19021 ttagctctaa cttcattggg ccggagctgc cggacattat ctcttcgctg gtatcactgc
    19081 tctgcctgac gctgttcctc aaacgctggc agccagtgcg cgtattccgt tttggtgatt
    19141 tgggggcgtc acaggttgat atgacgctgg cccacaccgg ttacactgcg ggtcaggtgt
    19201 tacgtgcctg gacaccgttc ctgttcctga cagctaccgt aacactgtgg agtatcccgc
    19261 cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga gtgggtgatc aacattccgg
    19321 tgccgtacct cgataaactg gttgcccgta tgccaccagt ggtcagcgag gctacagcct
    19381 atgccgccgt gtttaagttt gactggttct ctgccaccgg caccgccatt ctgtttgctg
    19441 cactgctctc gattgtctgg ctgaagatga aaccgtctga cgctatcagc accttcggca
    19501 gcacgctgaa agaactggct ctacccatct actccatcgg catggtgctg gcgttcgcct
    19561 ttatctcgaa ctattccgga ctgtcatcaa cactggcact ggcactggcg cacaccggtc
    19621 atgcattcac cttcttctcg ccgttcctcg gctggctggg ggtattcctg accgggtcgg
    19681 atacctcatc taacgccctg ttcgccgcgc tgcaagccac cgcagcacaa caaattggcg
    19741 tctctgatct gttgctggtt gccgccaata ccaccggtgg cgtcaccggt aagatgatct
    19801 ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa gagtctgatt
    19861 tgttccgctt tactgtcaaa cacagcctga tcttcacctg tatggtgggc gtgatcacca
    19921 cgcttcaggc ctatgtctta acgtggatga ttccttaatg attgttttac ccagacgcct
    19981 gtcagacgag gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc tggaagcggg
    20041 catgaagttg cccgctgagc gccaactggc gatgcaactc ggcgtatcac gtaattcact
    20101 acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg ctcagtcgac gcggcggcgg
    20161 gacgtttatt cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc agccgctaaa
    20221 aacgctgatg gccgatgatc cggattacag tttcgatatt ctggaagccc gctacgccat
    20281 tgaagccagc accgcatggc atgcggcaat gcgcgccaca cctggcgaca aagaaaagat
    20341 tcagctttgc tttgaagcaa cgctaagtga agacccggat atcgcctcac aagcggacgt
    20401 tcgttttcat ctggcgattg ccgaagcctc acataacatc gtgctgctgc aaaccatgcg
    20461 cggtttcttc gatgtcctgc aatcctcagt gaagcatagc cgtcagcgga tgtatctggt
    20521 gccaccggtt ttttcacaac tgaccgaaca acatcaggct gtcattgacg ccatttttgc
    20581 tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac cttagttttg ttcacaccac
    20641 catgaaacga ttcgatgaag atcaggctcg ccacgcacgg attacccgcc tgcccggtga
    20701 gcattatgag cattcgaggg agaaaaacgc atgattattt ccgcagccag cgattatcgc
    20761 gccgcagcgc aacgcattct gccgccgttc ctgttccact atatggatgg tggtgcatat
    20821 tctgaataca cgctgcgccg caacgtggaa gatttgtcag aagtggcact gcgccagcgt
    20881 attctgaaaa acatgtctga cttaagcctg gaaacgacgc tgtttaatga gaaattgtcg
    20941 atgccggtag cactggcacc ggtgggtttg tgtggcatgt atgcgcgtcg tggcgaagtt
    21001 caggcagcca aagcggcgga cgcgcatggc attccgttta ctctctcgac ggtttccgtt
    21061 tgcccgattg aagaagtcgc gccagccatc aagcgcccaa tgtggttcca gctttatgta
    21121 ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag caaaagcagc gggttgttcg
    21181 acgctggttt tcaccgtgga tatgccgaca ccgggcgcac gctaccgtga tgcgcattca
    21241 ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc aggcggtgac gcatccgcaa
    21301 tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt taggtaatat ctcagcttat
    21361 ctcggcaaac cgaccggact ggaagattac atcggctggc tggggaataa cttcgatccg
    21421 tccatctcat ggaaagacct tgagtggatc cgcgatttct gggatggccc gatggtgatc
    21481 aaagggatcc tcgatccgga agatgcgcgc gatgcagtac gttttggtgc tgatggaatt
    21541 gtggtttcta accacggtgg ccgccagctg gacggtgtac tctcttccgc tcgtgcgttg
    21601 cccgctattg cagatgcggt gaaaggtgat atcgccattc tggcggatag cggaattcgt
    21661 aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact tctgggtcgt
    21721 gctttcctgt atgcgctggc aacagcgggc caggcgggtg tagctaacct gctaaatctg
    21781 atcgagaaag agatgaaagt ggcaatgacg ctgactggcg cgaaatcgat cagtgaaatt
    21841 acgcaagatt cgctggtgca ggggctgggt aaagagttgc ctacggcact ggctccgatg
    21901 gcgaaaggga atgcggcata gtcgtttgag ccccctcacc ctaaccctct ccctcaggga
    21961 gtggggaccg ttcggcgctg tatgtactcc ctcactctga aacgacaccg cactcttttt
    22021 ttctccctcg cccctccggg gtgaggggaa aaggccgcac tgctcaccaa ttttctgcta
    22081 tcctgccccc gcactaaggg ggcagtatgc taaacatcgt actttacgaa ccagaaattc
    22141 cgccaaatac tggcaatatc atccgtcttt gcgctaatac cggctttcgt ctgcatatca
    22201 tcgaaccgat gggatttgcc tgggacgata agcgcctgcg ccgcgcgggg ctggactatc
    22261 acgagtttac cgccgttacg cgtcatcatg actttcgcgc attcctcgaa gcagaaaatc
    22321 cccagcgcct gtttgccctc accacgaaag gtacgcctgc tcacagcgcc gtaaactatc
    22381 aggatggcga ttatctgatg ttcggcccgg aaacacgcgg cctgccagcg agcattcttg
    22441 atgccctgcc cgctgaacaa aaaattcgca ttccgatggt gccggacagc cgcagcatga
    22501 acctgtccaa tgcggtgtca gttgtggtgt atgaagcctg gaggcagctg ggatatccgg
    22561 gagcgttgtt gagagattag ttactgtatg ccggatagat gattacatcg catccggcac
    22621 gatcacagga catcagatcc catccccata ctcaaatgta tggttaatac cgttgaaatg
    22681 ctggtccata tccattgatg gcttatcgct gtctggttta ccgacaatac gagccggaac
    22741 gccagcggcg gttgtatgcg gcggcaccgg ttgcagcacc acggaacctg cgccaatctt
    22801 cgcgccgcga ccaacttcaa tattgccgag gattttcgcg cccgcgccaa tcatcacacc
    22861 ttcacgaatt ttcgggtgac ggtcaccacc agatttaccc gtaccgccaa gcgtcacaga
    22921 ttgcagaatc gatacgtcgt tttcaattac cgccgtttca cccacaacga tgcccgtggc
    22981 gtggtcgagc ataatgccac ggccaatttt tgctgccggg tgaatatcga cctggaacgt
    23041 cacagaaacc tggttttgca gaaagattgc caatgcgcga cgcccctgat tccacaacca
    23101 gtgaccgatg cgataggcct gcaaggcatg aaaacccttc aggtataaca acggggttga
    23161 gtatttatcg actgccgggt cgcgggtacg caccgcctga atatcacagg ccgcagaggc
    23221 gatcatttcc gggtcagcgg cgtaggcttc ttccaccact tcacggatag caatagcagg
    23281 cataattggc gatgacagct tgttcgccag catgtagctc agtgcactac caaggttttc
    23341 gtgcttgagt agcgtcgcgt ggtaaaaact ggccagcatt ggctcacagt ccgccagcgt
    23401 tctggcttcg gctttaatat tgttccagac aatttccagt tcttcacacg acattgctta
    23461 ctccacacga tgagataatg accggcccgt tctgcgcggg ccgggtcata gcggtaacaa
    23521 aggttccctg gggttagtgg ctgctgcgct cgtccttgcg tgcacgacct aataacgtca
    23581 atgctgcctc gcgcgcgttt tttccgcaat ataatacttg ataaatttcc tcggttattg
    23641 gcatttcaac gccgaagcga tgcgccagtt cgcggacttc tttcgtattg cggtagcctt
    23701 ccaccacctg accaatcttc tcctgcgcgc tttgtacatc catgccctga ccgagcatca
    23761 tgccaaaacg gcggttacgc gactggttgt cggtacaggt aagcaccaga tcccccagcc
    23821 ccgccatgcc cataaaggtg gcaggatcgg cacccagcgc cgcgccaaga cgcgacattt
    23881 cagccagccc acgggtgatc agcgccgtac gcgcattcgc accaaaaccg ataccgtcgg
    23941 acatccccgc accaatggca atgacgtttt tcaccgcgcc gccaagctgc acgccaatga
    24001 aatccggatt gctgtaaacg cggaaacttt tgccgcagtg cagcagctgc tggagatcat
    24061 cggcaaaggt ctgatcggtc gaggccagcg aaatagctgt cggtaaacct gccgccagtt
    24121 ctttcgcaaa cgttgggcca gagataaccg ccagcggaat ttgatcgcct aacgcctcgc
    24181 gggccacgtc ctgtaacaga cgcccggttt ccgcttccag ccctttggtc gcccacacca
    24241 gacgcgcatc aggacgcatc agcggtttaa tctggcgcag cacttcacca aagacatggc
    24301 tgggtacgac gacgagaata ttacggctgg ctgccagcgc agtggcgaga tcgctttcaa
    24361 gatggagcgt atcgggaaaa ggcacatcgg ggagaaacgc ggcgttacag cggtcgcgtt
    24421 caagcgttgc gatatgttca gggtcatggc cccagaggac aacctcgtgg ccatttcttg
    24481 ccagggtgat ggcaagagcg gtgccgtacg agccggcacc gatcacagtc attgaagcat
    24541 tacgttggtt catcaggcat cctgatgttc ttcagtacct tcgccagcct gctgctgcaa
    24601 atagttcatg aacagcgcat cgaagttaac cggcgcaagg ttcagttgcg ggaatgtacc
    24661 gcgggatacc atgctggtga tgcactcacg agcatacggg aacagaatgt tcgggcagta
    24721 tgcgcccagg caatgtgcca tctgggtgcc ttcgataccc gcgatggaga aaataccgcc
    24781 ctgctgaact tcacacagga acgcggtttc ttcgcccagg gaggccgtta cggtaacacg
    24841 cagtaccact tcgtaaacgt catctgccag ttgagtagat gccgtatcca gatcaagttt
    24901 aacttctggc tgccaatctt tctggaaaac gtgcggcgcg ttcggcgctt cgaaagaaat
    24961 atccttggta taaatacgtt ggatctggaa agtcatttcg gtgttgtttt gttctgacat
    25021 gtgtagaaaa cccttaagtg ttgtccttaa atactgcgca atgtaatgcc aacgttcaca
    25081 cgttatttca gcaggggatc cagtccacca cgtgcatcca atgcatacaa gtcatcacag
    25141 ccgccaatgt gctgtgcgtc aataaaaatc tggggaaccg tggtgcgacc gctgcgtttg
    25201 atcatctctt cacgcttggc ggcattgcca tcgatcggca actcctggaa actcacgccc
    25261 ttgctgctca gcagtgcttt tgcacgatgg caatacgggc aggtttcttt agtatagatt
    25321 tcaacattgg ccataaatta gctcctgttt tttaccctga aatttcatgt tgcagggcgg
    25381 cagcaaaatg gagtgtcccc agaagcttat aacaataagt cgctgggggc ctaattgcag
    25441 ctaacggcct gcatcatgaa agacgacagg taaattattt gccgcgcacc aaaggcaagt
    25501 tttcgccagc ccagccagcg acgccctctt tcagcacgaa tacttgcgca aaaccagctt
    25561 tcgtcagcgc atttgcaggc tcctggcact gcaggccaga accgtctacc acgataaccg
    25621 gtttgtcttt gtgcttctca agctcaccaa cattgttggc tttgatttcg ctcggcaaca
    25681 gattgataga acctgcgata tggcctttac ggaagtcatc acgctgacgt aaatccacaa
    25741 ccacagcgtc ttctttgttg atcagacgcg tagcttcacc acgactaatc accttcactt
    25801 tcgaggtcag gctcttaaac gtagtcacaa gaaccgccac cagtaacgcg atccaggcga
    25861 tactcagtat gggatgacgg ccaacaaatt gcataatttc ttgcatgggg ggtaacaact
    25921 cccgacgtag tgattaaaaa aaccaggaaa ggagtatacc tgcgcggtgc ggcaaataca
    25981 gccagcgcgt taactggaat gcaattttgc ggggcgcgac gaaaaaaaag cgtaaatctg
    26041 gcatcggctt gccgcgagcg gtcgtatatt ttgatcttca gaggctattt tatcgattca
    26101 gctgtagtaa aattacgcaa attttgactc ttgagtatga ggttgtcgca atgtcggttt
    26161 ctaaaaaacc tatggtactg gtgattctgg atggctatgg ctatcgcgaa gaacagcagg
    26221 ataacgccat ttttagtgct aaaaccccgg taatggatgc actgtgggcc aatcgtccgc
    26281 ataccctgat cgacgcttcc ggtctggaag tcggtctgcc tgaccg
//
LOCUS       DAEVZA010000065        26012 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEVZA010000065 DAEVZA010000000
VERSION     DAEVZA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.065"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(114..2810)
                     /gene="mgtA"
                     /locus_tag="LAF92_002690"
     CDS             complement(114..2810)
                     /gene="mgtA"
                     /locus_tag="LAF92_002690"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBI9791676.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(2951..3004)
                     /gene="mgtL"
                     /locus_tag="LAF92_002691"
     CDS             complement(2951..3004)
                     /gene="mgtL"
                     /locus_tag="LAF92_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBI9791677.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            3189..4136
                     /gene="treR"
                     /locus_tag="LAF92_002692"
     CDS             3189..4136
                     /gene="treR"
                     /locus_tag="LAF92_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBI9791678.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            4255..5676
                     /gene="treB"
                     /locus_tag="LAF92_002693"
     CDS             4255..5676
                     /gene="treB"
                     /locus_tag="LAF92_002693"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBI9791679.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            5726..7381
                     /gene="treC"
                     /locus_tag="LAF92_002694"
     CDS             5726..7381
                     /gene="treC"
                     /locus_tag="LAF92_002694"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBI9791680.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQKINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            7775..9913
                     /gene="nrdD"
                     /locus_tag="LAF92_002695"
     CDS             7775..9913
                     /gene="nrdD"
                     /locus_tag="LAF92_002695"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBI9791681.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVKAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            10071..10535
                     /gene="nrdG"
                     /locus_tag="LAF92_002696"
     CDS             10071..10535
                     /gene="nrdG"
                     /locus_tag="LAF92_002696"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBI9791682.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNATQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(10580..10966)
                     /gene="cybC"
                     /locus_tag="LAF92_002697"
     CDS             complement(10580..10966)
                     /gene="cybC"
                     /locus_tag="LAF92_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBI9791683.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(11150..12502)
                     /gene="pmbA"
                     /locus_tag="LAF92_002698"
     CDS             complement(11150..12502)
                     /gene="pmbA"
                     /locus_tag="LAF92_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBI9791684.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTALMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            12596..13147
                     /locus_tag="LAF92_002699"
     CDS             12596..13147
                     /locus_tag="LAF92_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBI9791685.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(13303..14676)
                     /gene="mpl"
                     /locus_tag="LAF92_002700"
     CDS             complement(13303..14676)
                     /gene="mpl"
                     /locus_tag="LAF92_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBI9791686.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            14852..15850
                     /gene="fbp"
                     /locus_tag="LAF92_002701"
     CDS             14852..15850
                     /gene="fbp"
                     /locus_tag="LAF92_002701"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBI9791687.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(15883..16878)
                     /gene="yjfF"
                     /locus_tag="LAF92_002702"
     CDS             complement(15883..16878)
                     /gene="yjfF"
                     /locus_tag="LAF92_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBI9791688.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(16865..17887)
                     /locus_tag="LAF92_002703"
     CDS             complement(16865..17887)
                     /locus_tag="LAF92_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9791689.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(17901..19403)
                     /locus_tag="LAF92_002704"
     CDS             complement(17901..19403)
                     /locus_tag="LAF92_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI9791690.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(19713..20669)
                     /gene="ytfQ"
                     /locus_tag="LAF92_002705"
     CDS             complement(19713..20669)
                     /gene="ytfQ"
                     /locus_tag="LAF92_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBI9791691.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            20979..21509
                     /gene="ppa"
                     /locus_tag="LAF92_002706"
     CDS             20979..21509
                     /gene="ppa"
                     /locus_tag="LAF92_002706"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBI9791692.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(21589..21939)
                     /gene="chpB"
                     /locus_tag="LAF92_002707"
     CDS             complement(21589..21939)
                     /gene="chpB"
                     /locus_tag="LAF92_002707"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HBI9791693.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(21933..22184)
                     /gene="chpS"
                     /locus_tag="LAF92_002708"
     CDS             complement(21933..22184)
                     /gene="chpS"
                     /locus_tag="LAF92_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HBI9791694.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(22397..22738)
                     /locus_tag="LAF92_002709"
     CDS             complement(22397..22738)
                     /locus_tag="LAF92_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBI9791695.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(22741..>26012)
                     /gene="tamB"
                     /locus_tag="LAF92_002710"
     CDS             complement(22741..>26012)
                     /gene="tamB"
                     /locus_tag="LAF92_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBI9791696.1"
                     /translation="KPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKD
                     LFSRPVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDAL
                     DIDSNQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVN
                     LSGPVDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYT
                     LSMRTAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRG
                     ELTLNGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGT
                     LKGNSYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKG
                     LVKVRGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQP
                     DVNINLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTP
                     VGPWSLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDL
                     AMLKPFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVA
                     FQTLNLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMI
                     NPIFTRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNF
                     NGMRSTLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPD
                     VVFEATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPI
                     NSNLIVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIV
                     RKGELLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQA
                     ALSYLLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGD
                     SSQVVVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFE
                     F"
BASE COUNT         6142 a         7166 c         6607 g         6097 t
ORIGIN      
        1 tcctgcaatt tattgaattt gttggccgga taaggcattt acgccgcatc cggcatgaac
       61 aaagcttact ttgtctgcaa tctgaatcgg ggctatcgtg cccagtttat tctttactgc
      121 cagccgtaac gacggctata aaatcctttc accaactgag ttaatgtcat ataccctgcc
      181 agaatcgcaa ccagccacgg gaaatagctt aacggcagcg cctgcagctg cagataactg
      241 gccagcggtg aaaacggcaa tgcgatcccg acaatcatca cgatcacggt catgatcatt
      301 aacggccacg atgcacagct ctgaataaac ggcacacggc gggtgcggat catatgcaca
      361 atcagcgttt gcgacagtaa gcccaccaca aaccatcccg actggaacag cgtttgcgtt
      421 tccggcgtgt tggcatggaa tacccaccac atcaggcaaa acgtcagaat atcgaagatc
      481 gagctgatcg gtccgaagaa gatcataaag cgccccagat ccgccggatt ccaacgctgc
      541 ggcttttgaa tttgctcgtc gtcgacgtta tcaaacggga tcgccacctg tgacacatca
      601 tacagcaggt tctgaataag caagtgtaac ggcaacatcg gcaggaaggg caagaaagca
      661 ctcgccacca gcacgctgaa cacattaccg aagttggagc tcgccgtcat tttgatgtat
      721 ttcagcatgt tggcgaaagt gcgacgtcct tcaataaccc cctcttccag caccatcagg
      781 cttttttcca gcaggatgat atcagccgct tcacgggcaa tatctaccgc gccgtccaca
      841 gaaatgccga tatccgcagc gcgtaaggcg ggcgcatcat taataccatc gcccataaag
      901 ccaaccacat gcccttcgcg cttcagtaag gtcacaatgc gttctttatg catcggcgtc
      961 aggcgggcaa acagcgtggt gcgctgtgcg agatttgcca gttcgtcgtc agatagcgtt
     1021 tcaatatcac taccaatgac cacctctccc gcgtccagcc ccacttcatg gcacactttc
     1081 gctgcaacta actcactgtc gccagtaagg atttttacgg tgattccact cgcttttaat
     1141 gccttcagtg ccggagcggt tgtctctttt ggcggatcaa ggaaagcaat atatccttcg
     1201 aggatcaggt cggattcatc cgcccgctgg taatctcctt cacgcgctgg caggtatttc
     1261 gtcgccaccg caaccacgcg cagcccctga cgattaagcg tatcagtaac ccgcttaatc
     1321 ttacgcagca tgatgtcatc gagcggcaca atctcgccat tgtgacgcac ctgcgaacac
     1381 acattgagga tttcctgcaa tgcacctttg caaaccagct gatggtgctc ggtattttct
     1441 gccactacca ccgacatccg gcgacgctcg aaatcaaacg gaatctcatc aattttttgc
     1501 caacgactgg ccagcgagcg cgctgactct tcatccgtac cttcgagcac cgctgtatcg
     1561 agcaggtttt taagtccggt ctgataatga ctgttcaacc acgcgctatg cagcacgcgt
     1621 tcgctggttt taccggagat atcggtatga ttctccagca caattttgtc ctgtgtcagg
     1681 gtgccagttt tatcagtgca cagaatatcc attgcgccaa agttctgaat agcatccaga
     1741 tgtttgacga tcactttctg tttcgacagc tttactgcgc cgcgcgccag cgtcgaggta
     1801 acaatcatcg gcaacatttc cggcgttaag cctaccgcga ccgaaagcgc aaacagtgcc
     1861 gcttcccacc agtcgccttt ggtgtaacca ttgattaaca gcaccaccgg agccatcacc
     1921 agcataaagc gaatcagcag catactgacg cggctgatcc cttgctgaaa ggcattcggc
     1981 tcgctttcct gctcactaac acgccccgcc agttgaccaa accaggtatt ggcacctgta
     2041 gcaatcacca ttgcttgtgc cgtgccgctc accacggtgg tgcccataaa acacagggtg
     2101 tcacactcca gcggattgct gtgctccggc tggcgagtgg tagcggcttt ttctacgggc
     2161 agagactcac cggttaacga cgcctgagcg acgaacagat cccgcgcctg caagatacgt
     2221 aaatctgccg ggatcatatc ccccgccgcc agttttatta tatcgccggg caccagctgg
     2281 tcgatcggga tctccagcca gccattttcg cctttgtcgt taattacgcg cagaaccgtc
     2341 gctgtattgc tgaccatcgc tttcagggca tctgccgctt tagtggaacg tgcttcctga
     2401 ataaagttca gcaacgtaga aatagcgacc attagcgcga taacgcccgc ggcaaataaa
     2461 tcttccgtgg cgtaagaaat agcgccgaga atggtgagta aaatattaaa ggggttgcga
     2521 tagcagaccc ataaatgtac ccaccacggc gacggttgtt gtgcgggtaa tttattttca
     2581 ccatgttgtt cgcgggcaga ttccacttcc gcctgattta acccttccgg atgggtgtcg
     2641 aacgttttcc ataattcttc ttcgggcatc accgccattt tcaggcaatg cgcacttaag
     2701 gacggcggga ccgccgtatt cactgtctgc tgcgcgccag gcaatggatc gcgatgaacc
     2761 agacgggaag gtaaatggcg aatgagccgg gtaaaaattt ctttaaacat aaggagtccc
     2821 tccgcactgt ctgaaacagt acagaaatgt cttcaggcgt ggcgaagcct tacctgaagg
     2881 taataaagct gagcagggac gttaaaacgt cacggcctta cgcatccggt aacgccgtga
     2941 aaagacgggc ttaccggaaa agttttaatc tccgtcgagg gagaggcgtg ggatcaggtt
     3001 ccatataacc tccggtaagt gaaaaaagcg ccgcaaataa taaggagaat ccaataatat
     3061 tcgtagcatc tatggcgata taatacctgc tggcaactaa accaacgata aaccagactt
     3121 tacccattgc tgaatgcacg ggtaaagtta ggctcaaata attaaacaac acgttacagg
     3181 acaacaggat gcaaaatcgg ctgaccatca aagacatcgc gcgcttaagc ggcgtgggga
     3241 aatctacggt ttcccgggtg ctgaataacg aaagcggcgt gagccagcgc acccgcgagc
     3301 gtgttgaagc agtgatgaat cagcatggat tttccccttc ccgctctgcg cgcgctatgc
     3361 gtgggcaaag cgataaagtg gtcgccatca ttgttacccg cctggattcg ttgtcagaaa
     3421 atctcgctgt tcaaaccatg ctgccagcgt tctatgaaca aggttacgac ccaatcatga
     3481 tggaaagtca gttttccccg caattagttg ccgaacattt gggggtgctg aaacggcgta
     3541 atatcgacgg cgtagtgttg ttcggtttta ctggcataac agaagaaatg ttagcccact
     3601 ggcagtcatc gctggttctg ctggcgcgtg acgcaaaagg ctttgcttcg gtctgttatg
     3661 acgacgaagg ggcaatcaag atcctgatgc aacggctcta tgaccagggg catcgcaata
     3721 tcagttatct cggcgtgccg catagtgacg tgacgaccgg gaagcgacgt cacgaagcct
     3781 acctggcgtt ctgcaaagcg cataaactgc atcccgttgc cgctctgcca gggcttgcta
     3841 tgaagcaagg ctatgaaaac gtagcaaaag tgattacgcc tgaaactacc gccttactgt
     3901 gcgcaaccga cacgctggca cttggcgcaa gtaaatacct gcaagagcaa cgcatcgaca
     3961 ccttgcaact ggcgagcgtc ggtaatacgc cattaatgaa attccttcat ccggagatcg
     4021 tcaccgtaga tcctggttac gccgaagctg gacgccaggc ggcctgccaa ttgatcgcac
     4081 aggtaaccgg gcgcagcgaa ccgcaacaaa tcatcatccc cgccaccctg tcctgatcgt
     4141 ttcctgaacg ataaattgtg atcttcgctg cgtttcggga acgttcccgt ttttaaattt
     4201 ttccgcgcaa tatattctgc agccaaccaa aaatgtcatc tgccatgggg ctttatgatg
     4261 agcaaaataa accaaacgga tatcgatcgg ttgattgaac tggtcggcgg gcgcggcaat
     4321 attgcgacgg tgagccactg tattactcgc ctgcgctttg tcctcaacca accggccaat
     4381 gccagaccga aagaaattga gcaactcccc atggtgaaag gctgtttcac caatgccggg
     4441 caatttcagg tggtgattgg caccaacgtg ggtgattact atcaagcact aatagcgtca
     4501 accggacagg cgcaggttga taaagagcag gtaaaaaaag ccgcccggca gaatatgaaa
     4561 tggcatgagc agttgatctc tcatttcgcg gagatcttct tcccgttgct gcccgcgttg
     4621 attagcggcg gtttgatcct cggttttcgc aatgtgatcg gcgatttgcc catgagcaac
     4681 ggtcaaacgc tggcgcaaat gtacccttcc ctgcaaacga tctacgattt tctgtggttg
     4741 atcggtgaag cgatcttctt ctacctgccg gtcgggattt gctggtcagc ggtgaaaaaa
     4801 atgggcggca cgccgatcct tggtatcgtg cttggcgtga cactggtttc tccccagctg
     4861 atgaacgctt atctgctcgg gcagcagctg ccggaagtgt gggactttgg catgttcagc
     4921 atcgccaaag taggctatca ggcgcaggtg atcccggcac tgttagccgg gctggcgctg
     4981 ggcgttattg aaactcgcct taaacgcatc gtgccggatt acctctatct ggtggtggtg
     5041 cccgtctgtt cgctgatcct cgcggtgttc ctcgcccatg cgctgattgg tccgtttggt
     5101 cgcatgattg gcgatggcgt tgcctttgcg gtacgtcacc tgatgaccgg cagctttgct
     5161 ccgattggtg cggcattgtt tggcttcctg tacgcgccgc tggtgatcac cggcgtacac
     5221 cagaccaccc ttgctattga tttgcagatg attcaaagca tgggtggcac gccagtgtgg
     5281 ccgctgattg cgctgtcgaa tatcgctcag ggctccgccg taataggcat tatcatttcc
     5341 agccgcaagc acaatgagcg cgagatctcc gtgcctgccg ctatctccgc ctggcttggg
     5401 gtcactgagc ctgcaatgta cggcatcaac ctgaaatatc gcttcccgat gctgtgcgcg
     5461 atgattggtt ctggtctggc aggattgcta tgcggcctga acggcgttat ggcgaatggt
     5521 atcggcgtag gcggcctgcc gggaattctc tcgattcaac cgagctactg gcaggtgttt
     5581 gcgctggcaa tggctatcgc catcatcatc ccgattgtac tcacctcgtt tatctatcag
     5641 cggaaatacc gcctgggcac gctggacatt gtttaatttt cttcggggcg caattgcgcc
     5701 ccctcgcatt tgcaggaata acgtaatgac taatcttccc cactggtggc aaaacggcgt
     5761 tatctaccag atttatccaa agagttttca ggacaccacg ggtagcggca ccggcgattt
     5821 acgtggcgtt atccaacgcc tggactatct gcataaactg ggcgttgatg ccatctggct
     5881 aacccccttt tatgtctctc cccaggtcga taacggttac gatgtagcga actatacggc
     5941 gattgatccc acctacggca cgctggacga ttttgacgaa ctggtgacgc aggcaaaatc
     6001 gcgcgggatt cgtatcattc tcgatatggt gtttaaccat acctctaccc aacatgcctg
     6061 gtttcgcgag gcgctgaaca aagaaagccc ttaccgccag ttttatatct ggcgcgatgg
     6121 cgaaccagaa acgccaccga acaactggcg ttcaaaattt ggcggtagtg cgtggcgctg
     6181 gcatgcggaa agcgaacagt actatttgca tctctttgca ccagaacagg cggatctcaa
     6241 ctgggagaat ccagcggtac gcgcagagct gaaaaaagtc tgtgaattct gggccgatcg
     6301 tggggtcgac gggttgcgcc tggatgtggt gaatctgatc tccaaagacc cgcgtttccc
     6361 tgaagacctg gatggcgacg ggcgtcgctt ctacaccgac gggccacgag cacacgagtt
     6421 tttgcacgag atgaaccgcg atgtgtttac gccacgcggg ttaatgaccg taggtgaaat
     6481 gtcctccacc agccttgagc attgccagcg atacgcggca ctgacaggca gtgaattgtc
     6541 gatgaccttt aattttcatc acctgaaggt cgattatccc ggtggtgaaa aatggacgct
     6601 ggctaaacct gactttgtgg cgttgaaaac attgttccgc cactggcaac aaggaatgca
     6661 caacgtggca tggaatgcct tgttctggtg taaccacgat cagccgcgca ttgtttctcg
     6721 ctttggtgat gaaggtgaat accgcgtgcc tgcggcaaaa atgctggcga tggtgctgca
     6781 tggcatgcag ggaacgccgt atatctacca gggcgaagag attggcatga ccaacccgca
     6841 tttcacgcgc attactgact atcgcgacgt ggagagccta aatatgtttg ccgagctgcg
     6901 caacgatggt cgtgatgccg acgagttatt ggcaatcctc gccagtaaat cccgtgacaa
     6961 cagccgcacg cccatgcaat ggagcaacgg cgataatgcc gggtttacgg ctggcgaacc
     7021 gtggattggc ctgggcgata actatcaaaa aatcaacgta gaagccgcgc tggccgatga
     7081 gtcctcggtg ttttacacct accaaaagtt aatcgcactg cgtaagcaag aagccatcct
     7141 gacatggggc aattaccagg atctgctgcc aaacagccct gtattgtggt gctatcgccg
     7201 tgaatggaag gggcaaacct tgctggtcat tgccaacctt agccgtgaga tccaaccctg
     7261 gcagccaggg caaatgcgcg gcaactggca gcttgtgatg cataactacg aagaagcctc
     7321 accacaaccc tgtgccatga atttacggcc ttttgaggct gtctggtggt tacagaagta
     7381 aatcttccct aagccccggt aatgccggtc attccggggt tttgtctgtc agcatgttgt
     7441 ttttgttgat ttttcaacca gcaaattcat taaaaaattt acataacgct gtagcgcccg
     7501 tcatccgtac gctctgcttt ttactttgag ctacatcaaa aaaagttaaa acatccttga
     7561 tgcaaagcac tatatataga ctttaaaatg cgtccaaacc caatatgttg tattaatcga
     7621 ctataattgc tactacagct ccccacgaaa aaggtgcggc gttgtggata agcggatggc
     7681 gattgcggaa agcaccggaa aacgaaacga aaaaaccgga aaacgccttt cccaatttct
     7741 gtggataacc tgttcttaaa aatatggagc gatcatgaca ccgcatgtga tgaaacgaga
     7801 cggctgcaaa gtgccgttta aatcagagcg catcaaagaa gcgattctgc gtgcagctaa
     7861 agcagcggaa gtcgatgatg ccgattattg cgccactgtt gccgcggttg tcagcgagca
     7921 gatgcagggc cgcaaccagg tggatatcaa tgagatccag accgcagttg aaaatcagct
     7981 gatgtcaggt ccatacaaac aactggctcg tgcttacatc gagtaccgtc acgatcgcga
     8041 cattgaacgt gaaaaacgcg gtcgcctgaa ccaggagatc cgtggtctgg tcgagcagac
     8101 caactcctcg ttactcaacg aaaacgccaa caaagacagc aaggtgattc caacccagcg
     8161 cgacctgctg gccgggatcg tggctaaaca ctatgcacgc cagcacctgc tgccgcgtga
     8221 cgtggtgaag gcacatgagc gtggcgatat tcactatcac gatctcgatt actcgccgtt
     8281 tttcccgatg ttcaactgca tgttgatcga cctgaaaggc atgctgaccc agggctttaa
     8341 gatggggaac gccgagattg aaccgccgaa gtcgatctct acggcaaccg cggtaactgc
     8401 gcagattatt gctcaggtcg ccagccatat ttatggcggc accaccatta accgtatcga
     8461 tgaagtgctg gcgccgtttg ttaccgccag ctacaacaaa catcgcaaga ccgcagaaga
     8521 gtggaacatc ccggacgccg aaggctacgc aaactctcgt accatcaaag agtgctacga
     8581 tgccttccag tcgctggagt acgaagtaaa caccctgcac accgccaacg gtcagacgcc
     8641 gtttgtaacc tttggttttg gtctgggcac cagctgggaa tcgcgcctga ttcaggaatc
     8701 gatcctgcgt aaccgtatcg caggcctcgg taaaaaccgt aaaactgcgg tgttcccgaa
     8761 actggtgttt gcgattcgcg atggtctgaa ccataaaaaa ggcgatccga actacgacat
     8821 caaacagctg gcgctggagt gcgcaagcaa gcgcatgtat ccggatatcc tgaactacga
     8881 tcaggtagtg aaagtcaccg gttcgtttaa aaccccgatg ggctgccgca gcttcctcgg
     8941 cgtgtgggaa aatgaaaacg gcgagcagat ccacgatggt cgtaacaacc tcggcgtgat
     9001 cagcctgaac ctgccgcgta ttgctctgga agcaaaaggc gatgaagcca ccttctggaa
     9061 gctgctggat gaacgtctgg tgctggcacg taaggcgctg atgacccgta tcgctcgtct
     9121 cgaaggcgtg aaagcgcgcg tcgccccgat cctctatatg gaaggtgctt gtggcgtacg
     9181 tctgaatgct gacgatgatg tttctgaaat cttcaaaaac ggtcgtgcgt ctatttcgct
     9241 gggttacatc ggcatccacg aaaccattaa cgcgctgttc ggcggcgagc atgtttacga
     9301 caacgagcag cttcgcgcga aaggtatcgc cattgttgaa cgtctgcgtc aggcagtgga
     9361 tcagtggaaa gaagaaacgg gttatggttt cagtctctac agcacgccga gtgaaaacct
     9421 gtgcgaccgc ttctgccgtc tcgatactgc tgagtttggc gtggtgccgg gcgtgaccga
     9481 taaaggttac tacaccaaca gtttccacct cgatgtggag aagaaggtga acccgtacga
     9541 caagatcgac tttgaagcgc cttacccgcc gctggcgaac ggtggtttca tttgctacgg
     9601 cgagtatcca aacattcagc acaacctgaa ggcgctggaa gatgtctggg attacagcta
     9661 tcagcatgta ccgtattacg gcaccaatac accgattgat gagtgctacg agtgtggctt
     9721 taccggtgag ttcgagtgca ccagcaaagg cttcacttgc ccgaaatgtg gtaaccatga
     9781 cgcctcccgt gtgtcggtaa ctcgccgcgt gtgcggatat ttaggtagcc cggatgcacg
     9841 tccgtttaac gctggtaagc aggaagaagt taagcgccgc gttaaacatt tggggaatgg
     9901 gcagataggt taatcttcac tctgcgctaa gcaaatgccg gatgcggcgt aaacgcctta
     9961 tccggcctac atttggcaac ggctccaggc ctgataagac gcgacagcgt cgcatcaggc
    10021 attgattacc ggatgcagcc tacacgcgga ctgataagcc aggcgaaata atgaattatc
    10081 atcagtacta tcctgtcgac atcgtcaacg gccccggcac tcgctgcacc ctgtttgtct
    10141 ccgggtgtgt tcatgaatgc cccggttgct ataacaaaag cacatggcgg gtaaattctg
    10201 gtcagccatt taccaaagca atggaagacc agatcattaa cgatctgaat gacactcgta
    10261 tcaaacgcca gggtatttcc ctctccggcg gcgatccgct gcatccgcaa aacgtgccgg
    10321 atattctgaa actggtacaa cgcatccgcg ccgagtgtcc gggtaaagac atctgggtgt
    10381 ggacaggcta taaactcgac gaactcaacg ctacgcaaat gcaggttgtt gatttgatta
    10441 acgtgctggt cgacggcaag tttgtgcagg atttaaaaga cccttccctg atctggcgcg
    10501 gcagcagcaa tcaggtggtg catcatttgc gatgataagt tccctgttac ctgagtgcag
    10561 gcaacaggga aatgaggaat taacgatact tctggtgata ggcgttgcgg gtcgttttca
    10621 gttgctctgc ggcggcctgc gcttctttta ctttaccttc atttgccagc ttcagcgcgt
    10681 cgtcaatctg accgaccaga atgtcgaaac cgtggcggaa atctttcatt tccgggctgt
    10741 ccggtgattt atcttcgagc ttcggcggcg ttgctttttg cgcatccagc gctgcggcgc
    10801 gcatcatcgt taacgcgtct ttgacttgcg ccgcgttatc cgctttttcg atcactttta
    10861 aattgtcgtt gagggtttcc atattgtctt caagatcagc ggcaaacgac gcagaactga
    10921 ataccaacga ggagactgca agaatagcta acaggctttt acgcattgct cacttccttt
    10981 tttatttaca cttcatcctt cacactgcct cattgacggc tacgctcatt cgcccgagtc
    11041 acgtactgcg agtacgctcc ccggacctca ttcactttcc gtctcgatgc agtgcgaatg
    11101 attttgtgta attttttagt ttatagcgcg gcaggtcgcg ccagtttttt tactgtccgg
    11161 cgattttcat ctccggcaac agcacagaac cacactgtat attactgcgt gtttcaatat
    11221 cgttaccgac ggtgacaata ttgcgccaca tatcttttaa attacctgcg atggtgattt
    11281 cgctaaccgg atactgaatt tcgccgtttt ctacccagaa gcccgccgca ccacgggaat
    11341 aatcaccggt aattgcactc acgccctggc ccatcaatgc cgtcaccacc agcccggtgc
    11401 ccatctcttt gagcatttgt tcgaagctta gcccttgtcc ggcaatccgc cagttgtgaa
    11461 taccgcccgc atgtccggtg cttttcagcc ccagtttccg cgccgagtag ctggtcagca
    11521 gccactgagt caggatgcca tctttaataa tatcgcgacg ctcggtgcgc acaccttcgc
    11581 tgtcgaatgg cgtcgacgcc agccctttca gcagatgcgg atgctcttca atggtcagcc
    11641 agtccggcag aatttgttta cccagcgaat ccagcagaaa ggtagattta cggtaaaccg
    11701 agccacccgc tatcgccccc accagatggc caaaaagccc ggttgccact tcattggcaa
    11761 aaatgactgg cgctttcatg gtggagagtt tacgcggtga caggcgcgat aaagtacggc
    11821 gagcacagtc ggccccaacc cactctggcg tttgcagatc gctcatcgca cgaccaatgg
    11881 tgtaggcgta atcacgctcc atatcgccat tttcttcggc aattacacag ctggagagtg
    11941 aatgacgcgt agagcagtaa ccctgcaaca tgccgtggct gttgccaaaa actttgacac
    12001 cgtagtggct gttaaaactg ccgccttcgg tattggtaat gcgtttgtcc gcctgcaatg
    12061 ccgcctgttc tgcgcgggcc gccagttcaa tggcttcatc cggggaaact tccgcagggt
    12121 ggaacaagtc gagatctggt gcgtcaaagg ccagcagctc tttgtctgcc acgccggcac
    12181 agggatctgg cgaggtataa cgggcaatat ccagcgccgc ctgtacggtg cgggcaatgg
    12241 cctgcgggct taaatcggtg gatgatgcgc tacctttgcg gttctggtga taaacagtga
    12301 tccccagcgc gccatcgcta ttgaattcaa cattctccac ttcaccataa cgcgtgctta
    12361 cgctaatgcc ggtggtcttg ctgacggcaa cttccgcacc gtccgatttg cctgaggcca
    12421 actccagcgc agtggaaact gcttcttcca gaatcttgcg ctgcgcttca acttgagaga
    12481 ttactttcat tgcaagtgcc ataatgtaga gagtttctct gaagtctaac agagaaccgt
    12541 ttttcagtgc gcatcttaac tggtaacatt agcctctttt tttaaggagc ctgagatgac
    12601 taagcagccc gaagactggc tcgacgacgt tcccggtgat gacatcgaag acgaagacga
    12661 tgaaattatc tgggtcagta aaagtgaaat taaacgtgat gccgaggagc taaaacgcct
    12721 tggcgcggaa attgttgatc tggggaaaaa cgcgctggat aagatcccgt tagatgcgga
    12781 tctgcgcgcg gctattgagc tcgcccagcg tattaagatg gaaggtcgcc gccgccagtt
    12841 gcagctcatc ggtaagatgc tgcgccagcg cgatgtagaa ccgatccgtc aggcgctgga
    12901 taagctgaaa aaccgtcaca accagcaggt ggtgctgttc cataaactgg aaaacctgcg
    12961 cgatcgtctg atcgatcaag gtgatgacgc catcgctgag gtattaaatc tgtggcccga
    13021 tgccgatcgt cagcagctgc gtactctgat ccgcaacgcg aagaaagaga aagaagggaa
    13081 taagccgccg aaatccgcac gccagatttt ccagtatctg cgcgaactgg cggagaatga
    13141 agggtaattt tcgttgttga tgtgagtatg gtttgccgga tgcggtgcac ctgtaggcct
    13201 gataagacgc atcaagcgtc gcatcaggca ctatgctcaa atgccggatg cggcgtgaac
    13261 gccttatccg gcctacgcac actatatcag gctgaggccg aattactgcg cagcttccgc
    13321 cttcttcgcc agaccatcca gcagtttctg atggatccca ccaaaaccgc cgttgctcat
    13381 caccagaata tggtcgccag gctgagcggt tttcaccacc atatctgcca gcgtatccac
    13441 atcgccactc cagtgtgcag gctgaacgca ggcttctgcc acttctgcca cctgccacgg
    13501 aatatgcgcc ggttgcagca ggaagacttc atcggcacga cctaatgaag gtgccagatc
    13561 gtctttgcag atccccattt tcatggtatt cgagcgcggt tccagcacag caataatgcg
    13621 cgccgtacca ccaactttgc cacgcagcgc cgccagcgtt gccagaatcg ccgtcgggtg
    13681 atgggcaaaa tcgtcatata ccgtgacgcc attcgcttca ccacgcaact ccagacggcg
    13741 acgagcatta ataaacgaac ccagcgcgtt agcggcatct gccggcgcta caccaacatg
    13801 gcgagccgct gcaatcgcca tcaggccatt gtgcatatta tgttcgccta ccagcgacca
    13861 tttcacttcg cccacttttt cgccatccag caaaacttcc cattcggaag catcggtggt
    13921 cagctttttc gcctgccagt gaccctgctc acccaccagc tcctgctcgc tccagcagcc
    13981 catcgccatg gtctgtttca ggttgatgtc attttccggc cagataatac ggccctgccc
    14041 cggaacgata cgcaccagat ggtggaactg tttctggatc gctttcaggt cgtcaaagat
    14101 atcggcgtga tcgaactcaa ggttgttgag gatcagcgta cgcgggcagt aatggacaaa
    14161 tttagagcgt ttgtcgaaga aggcgcagtc gtactcatcc gcttcgataa caaagaagtc
    14221 gctttcgccc agacgcgccg aaacctcaaa gttccccggc acaccgccga ttacaaagcc
    14281 cggtttgtaa ccacactgtt ccagaatcca ggtcgccatt cccgcggtgg tggttttgcc
    14341 gtgtgtacct gcaacggcca gcacccagcg gtcgcgcagc acaaaatcgt gcagccactg
    14401 tggacctgac atataaggga tgtttttttc cagtaccgct tccacacacg gatttccacg
    14461 ggtcatggcg ttgccaataa tcaccagatc cggctgcgga tcgagctggc tggcatcgta
    14521 accctgaatc agctcaatgc cttgcttctc aagtaaggtg ctcatcggcg gatacacatt
    14581 ggcgtccgaa cccgttactt catggcctaa ctggcgcgcc agcatcgcca gaccgcccat
    14641 aaacgtgcca caaattccta aaatatgaat gcgcatacgt cactatcctt ttttaatctg
    14701 ccgctcattt tacgcatatg tcgggcaagt gagaaacgca tttcaggata atccgcaatt
    14761 tgctggcgcg attcacctga gcgcaacatt gagattattt gttaagattg ttgcggtcgc
    14821 tttactccat aaacattgca gggaaagttt tatgaaaacg ttaggtgaat ttattgtcga
    14881 aaagcagcac gagttttctc atgctaccgg tgagctcact gctttgctgt cggcaataaa
    14941 actgggcgcc aagattatcc atcgcgatat caacaaagca ggactggttg atatcctggg
    15001 tgccagcggt gctgagaacg tgcagggcga ggttcagcag aaactcgact tgttcgctaa
    15061 tgaaaaactg aaagccgcac tgaaagcacg cgatatcgtt gcgggcattg cctctgaaga
    15121 agaagatgag attgtcgtct ttgaaggctg tgaacacgca aaatacgtgg tgctgatgga
    15181 ccccctggat ggctcgtcca acatcgatgt taacgtctct gtcggtacca ttttctccat
    15241 ctaccgccgc gttacgcctg ttggcacgcc ggtaacggaa gaagatttcc tccagcctgg
    15301 taacaaacag gttgcggcag gttacgtggt atacggctcc tctaccatgc tggtttacac
    15361 caccggatgc ggtgttcacg cctttactta cgatccttcg ctgggcgttt tctgcctgtg
    15421 ccaggaacgg atgcgcttcc cggagaaagg taaaacctac tccatcaacg aaggaaacta
    15481 cattaagttt ccgaacgggg tgaaaaagta cattaaattc tgccaggaag aagataaatc
    15541 caccaaccgc ccgtatacct cacgttatat cggttcactg gtcgcggatt tccaccgtaa
    15601 cctgctgaaa ggcggtattt atctctaccc aagcaccgcc agccacccgg acggcaaact
    15661 gcgtttgctg tatgagtgca acccgatggc attcctggcg gaacaagcgg gcggtaaagc
    15721 gagcgatggc aaagagcgta ttctggatat catcccggaa accctgcacc agcgccgttc
    15781 attctttgtc ggcaacgacc atatggttga agatgtcgaa cgctttatcc gtgaattccc
    15841 ggacgcgtaa tctcctgcgc ctctccacac ggagaggcgt ttttatcgct gcgcaatgtt
    15901 gactcttgtc actggcgaac tctgacgatt ctcccacagc accgttaatc cacgctgtaa
    15961 tgctataaaa ataaacaaca aaataccgat ggcgattttc gtccaccatg agctcagcgt
    16021 gccatcaaag tttatgtaag tctgaatcag tccctggatc gccacgccaa aaagcgtccc
    16081 taataccgtt ccaacgccac cgctcaaaag cgtaccgcca attaccactg aggcgatagc
    16141 atccagttcc acacctacgc ccgccagcgc atatccggcc tgggtataaa tcgagaagac
    16201 aatccccgcc agcgttgcca gtccggtgga gagcatataa atgcgaatag tggtgctgcg
    16261 ggtagaaatc cccatcaggt ttgccgacgt tgcgttgccg ccaatggcgt atacctgatt
    16321 accaaaacgg gtacgatgcg cgaggaatat gccgataacc accaccgcca gcatcagcag
    16381 tcccatcgca cttaagcgac cgccgccagg gattttccac gcaaggcttg agagcgtgtc
    16441 ataaatggga tggtttatcg gaatcgactc ttccgaaacg agatagctga cgccgcgcaa
    16501 aaagaacatt cctgccaaag taatgataaa cgccgggatc ttcagggcgt cgatcaaaag
    16561 ccccataaat gcgccaaagg cacagcccat caccagcacc agcggaaatg ccagcaacgg
    16621 cgagaggccg aagtcgccaa tcacttttgc cagaaacacg ccggtaaaag cgatcaccga
    16681 accgacggag agatcgatcc caccggagag gatcacaaag gtcatgccaa cggcaatgat
    16741 cccaagaaag gcgttatcgg tcaggatatt gcagatcact cttgtggaag caaaaccagg
    16801 aaactgcgtc aggcagtaga gatagcccag cacaaagacg ccgatggtga tcatcagtgg
    16861 caaattacgt tttatcacgg ctacgtaccc ctttaatcag actgataaag cgttgcgact
    16921 ggacaatcag cacgcaaagc accaccaccg ctttcaccac ctggttcatc tctggcggaa
    16981 agcccgaaag caaaattccg gtgttcatcc cctgaataat cagcgccccc accaccgaaa
    17041 gcagcaggtt aaagcgcccg cccatcagcg atccgccgcc aatcaccacc gcgagaatgg
    17101 cgtccagctc cagccataat ccggcgttgt tggcatcggc accgcgaata tccgccgcca
    17161 cgataatgcc cgcaatcgcc gcacatagcc cgctcaacac gtaggtgagc atgacgatga
    17221 ttcgcgtgtt gactccggca ttttttgccg cccgaatgtt gataccaacg gcttcgataa
    17281 acatccccag tgccgttttg cgggtcaaca gccagaacag gagaagagtc agtaccgcaa
    17341 taatgactgg cgttggcagg aacaacagcg atccactgcc aaaccatgag agatccggcg
    17401 agttaaacgt gacgatctgg ccggaggtga tcaattgcgc cacgccgcgc ccggcgacca
    17461 tcaggatcag ggtggcgaca aacggctgaa ttttgaggat cgctaccagt atgccgttcc
    17521 acaatcccgc caggatgccg gtgcccaggg cgcttaacaa aacaatcggc aggctgaatc
    17581 ccgcgaccgt catcgcagcc gttgtggctc cggcgatagc catcaccgcc cccacagaga
    17641 gatcgatacc accggtggcg atcaccagcg tcataccaat cgccagtaac gcaacgggag
    17701 ctgcacggtt aagaatgtcg atagggctac cgaacaaacg cccatcctga agcaccacct
    17761 gccagaaatg cggggctacc aggctatcga ccagcagcac cagcaacagt gccgccagct
    17821 gcggcatccc cgtcggccag tgaaaacgcc tttttggcgg cgtagtgtca gggagagatt
    17881 gaggcatcac actgttctcc ttacgccgca atggcgttca tgatcgccgg aacggaaagc
    17941 tgtgccagcg ggatctccgc cacctgtttg cgatcgcgca tgatgatcac ccggtcggca
    18001 tagcccacca gttcttccag ttcggaggag atcaccagca gcgccaggcc gtcagcacac
    18061 agcgtttcaa tcaggcggat gatctcggcg tgggcaccaa catcaatgcc gcgggttggc
    18121 tcgtcgagga tcagaaattg cggtcgggtc agtagccaac gtgaaagcaa cactttttgc
    18181 tgattgccgc cagagagaaa ttcaatcggt tgttcagttg aaggtgtgcg aatgccaagc
    18241 tggcggataa agcgttcggc aatctcttgc tgctctttac gggaaatggg tcgtagccag
    18301 ccccgctggg cctggagagc gaggatgata ttttcccgca ccgaggcggc agcgatgatg
    18361 ccatcggttt tcctgtcttc cgggcagaag ccgatgccca gtaccgaagc ctgatgtggc
    18421 gatcgcaggg tttgcggttt gccttttatc aacgccgtgc cgctgtcagc aggtttgata
    18481 ccgaagatca cttcggcggt ttcggtacgt cctgatccca gcaagcctgc cagtccgacg
    18541 atctcgccgg ggcgcacttc gagatcaaac ggtgcgatcg ttcctttttt gccgtaattt
    18601 ttgaacgcgg caacgggttt gtcgctcaac aatgttcgcc cggcacgctg tagcgcgtgg
    18661 gtatccagct cgcgccccag catcattttt accagttcga tctgcggtag ctcgcgcgtt
    18721 tcccgacagc ctacgaaact gccgttgcgt aagacagtga tccgatcgct gacctgatag
    18781 acctgatcga gaaagtgagt gacaaagatc aggctgacgc cgcgatcgcg caactgacgc
    18841 atcaggccaa ataatagctc cacttcctgg gtgtcgaggc tggcggtggg ttcatcgagg
    18901 atcagcactt tggcggagag atcgatagcc cggcaaatgg cgacgatttg ctgcatcgcg
    18961 actgaaaagc ggttaagcgg ttcgcgcacg tcgagggaga aaccgtaaga tgccatcagt
    19021 tcggtggcgc gcttttccat ctctttacgg cgtagaaggc cgaaacgttt gggttcgcga
    19081 cctatgaata gattatcagc gaccgacata ttgggtagca ggttgacttc ctgataaacg
    19141 gttccgatgc cgagttgttg tgcatgggcg gtatttttcg gtgagatagc ctggccttcc
    19201 agccagatgg tgccgcgatc ggcgtggtat acaccggtta atgctttgat tagcgtcgat
    19261 tttcccgccc cgttttcacc gagcagcgcc atgatttcgc cacggcgcag gctgaaatca
    19321 acgttgtcta acgctttgac gccggggaaa aatttactta atccttcggt gcggaggatt
    19381 tcctggtgtt ggtcggtcgt catggtttcc cccttagcca cgctctaaaa gtctgggaat
    19441 agatcgtgcc tgatgttaag agctgcgccg tttgtccccc tcaccctaac cctctccccg
    19501 aaggggcgag gggacagatt gtgcacggtg ttaagtccgg tgttgcacca ctgccggatg
    19561 cggcgtgaac gccttatccg gcctacacag agcaccgaac tcgtaggcat gataagacgc
    19621 ggtaagcgtc gcatcaggct tcagcgcacc accgccggat gcggcgcgag cgccttatcc
    19681 ggcctacgaa tcggcatcag gcatagcaac cctcaatacc ccatattttt cttcttctct
    19741 aactcttctt ttgcggtatc aggcaggtaa agggtggatt tggttaacgt cagcttttca
    19801 ggcatggtgc cgtctttttt gtatttctcc agcgcgtcaa aagcgggtcc cgccatattc
    19861 ggcgtcagtt caacactggc gttcgcttcg ccatcaatca tcgctttgta gatgtccggt
    19921 acaccgtcaa tggaacccgt gaggatatct ttgcccggtt tcaggcccgc ttctttaatt
    19981 gcctgaattg cgccaatcac catgtcatcg ttatgggcgt aaaccatgca gatgtttttg
    20041 ccgttgtttt ccgctttgat aaagctttcc atgacttctt tgcctttact gcgggtaaag
    20101 tcacctgact gcgagcggat aattttgata tttggcgcat tcttaatggc ttcggcaaag
    20161 cctttcttgc ggtcaatggc gacgctggcc ccaacggtgc cctgcagctc caccacgttg
    20221 catggtttgc cattcacttc ttttaccagc cagtcaccga tcaacttacc ttcgaggatg
    20281 ttgtcggcag taacggtggt catatagaga gatttgtctt tcacatcgat ggaacggtcg
    20341 agcaagaaga ccgggatttc ggcatctttc gcctctttta ataccggttc ccaacctgtc
    20401 gcgaccaccg gagcgataaa gatcgcatcc accccttgcg cgacgaagga acgtaccgct
    20461 ttaatctggt tttcctgctt ttgctgacca tcggcaattt tcagcgtaat tccgcgtttt
    20521 tcggcctcac ttttcgccac attggtttct gcagcgcgcc agccggattc cgatccgacc
    20581 tgcgaaaatc ctacggttaa tggagcggcc aacgccatag acgacatggc tgccgagact
    20641 gcagagacta caagtaagcg tttccacata gggttgtcct cgtcggggta attattattg
    20701 gtgaaaagat gttcgcggaa aaactataga caattcgtta tgtaacggat tgcgttacat
    20761 cacacttcat aaaagtaaat aaaacgttaa tcacaagttt gtaatcgctt tcatctcact
    20821 atgaaaaatg cggctacggt tatggatttt cctgctctgt ataccgtctt aaaactggcg
    20881 aaaaaggaaa atgaagacga aaacaagcga agacattcgg cgcgagttgg ctataatact
    20941 cggcacttgt ttgccacata tttttaaagg aaacagacat gagcttactc aacgtccctg
    21001 cgggtaaaga tctgccggaa gacatctacg ttgttattga gatcccggct aacgcagatc
    21061 cgatcaaata cgaaatcgac aaagagagcg gcgcactgtt cgttgaccgc ttcatgtcca
    21121 ccgcgatgtt ctatccgtgc aactacggct atatcaacca cactctgtct ctggacggtg
    21181 acccggttga cgtactggtc ccgactccgt acccgctgca gccgggttct gtgatccgtt
    21241 gccgtccggt tggcgttctg aaaatgaccg acgaagccgg tgaagatgcg aaactgattg
    21301 ctgttccgca caccaagctg agcaaagaat acgatcacat taaagacgtt aacgatctgc
    21361 cagagctgct gaaagcgcag atcgctcact tcttcgagca ctacaaagac ctcgaaaaag
    21421 gcaagtgggt gaaagttgaa ggttgggaaa acgcagaagc cgctaaagct gaaatcgttg
    21481 cctccttcga gcgcgcaaag aataaataag ttcttatagc gcaataaccc tgaacaccgg
    21541 gcttcggtta gtaagggttt ttttatgccc gcgataaata aacacacctt attccaccac
    21601 cgcctgcaag cgtaataacg cctcttccac cacctcatcc gcagccagac caatacgctt
    21661 tgccagccgg gcgcgtagat ccatcatccg cacctgattc accagcacca cgccgtgcac
    21721 atcgccttct tcgcaatgta aaggaacgct aaatccggca taacgggcaa aatttccgcc
    21781 ctgcgtaatg ggggccacca gcgtcattcc cagttgatta aaggcttgaa cggagagcac
    21841 aagcgcaggt cgaccagcac cttgctgttc atggccgctt gctggatcaa agccaaccag
    21901 cacaatgtct ccccgttcaa attcactttt ctttaccata tttcgtcacc cgcaggggtg
    21961 gatttacccc agacatcctg ctcgctaagt tccgcggcgt tcatgtcaca ctgtgccagc
    22021 agttcatcaa gcgagtagcg cctggagatg ggtgtcagaa tcagttggtt gttgctcacc
    22081 tgcgcttcca cgctctgccc cggctgtaag ttaagttctt tcattacgat attgggaatg
    22141 accatacctg cactgttccc ccatcttttt atggtaatac gcatctgcac ctccttaagt
    22201 tatacaaagt ataactcacg gtgctacagg aaacgtttta cccctgcaca tttgtgaacg
    22261 ccagcagagt tttttatgcg attgcagata tggaaagcgg ctcggagaga aaagcaaaag
    22321 atggaaaagt tacaaaaaaa aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg
    22381 tggcgtgctt ttattattac ttatccctgt ctaaccagtc gccgctttca attagcttta
    22441 atccatcgac gggtcgttga taaacgtaca tccatgcact cccgtacggc gtctgaatca
    22501 actggcgcgc gtattcaccg cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt
    22561 tgtcaatacg ataaacttca ccgtgtactg ttccgttccc cggaactgcg cctggatagt
    22621 ggcccaggct atacaactgg tagttatcga tactgaaatc gcccagtaac tgggcattgg
    22681 tcatccagtg actgttgcct tgtttgtggc gtaaactgcc gtagacaaat attcgcattg
    22741 ctaaaactcg aactgataga gcaaatccag tgcctggtct acaccagaca cggcttccag
    22801 atatagctta ggcatcaggc gataacgtaa cgtgagtgtt gctatagagt caaatatacc
    22861 cacgccgtat ttcacttgca gacctggcaa tacatagccg ctgaccacta cctgggagga
    22921 gtcgcctact ccctgggtgt cgagcgctaa attgcttacg ccaaacgtct cgccgatttt
    22981 acccacaatc tggccacttt gcgcaacccc caggccaatc agcattgagg tcatcgccgc
    23041 actgtcgctc tgatcgctct ccagcccttg tccacgtagc aaataagaca atgcagcttg
    23101 ttgcgacata gccgggtcag agaagatctc cgctttcggt tcgtccgcca gaccagtgac
    23161 gcgaactccg gcgattacgt cgtcttctgt agcatccggg ttacgaatag cttcaatatt
    23221 aagatacggt tgatccggcg gaccagagaa cagtaactca cccttacgca caatcagatc
    23281 ctgaccatag gcatggaagc gcccttcagg gatgttgatc tgcccgttca ggcccagccc
    23341 ttgtttgtcc tgaacgacgt taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc
    23401 aatgcgcaca ttattgccaa cgtggacaat caggttactg ttaatcggaa tcgacgccgt
    23461 tttcggctct tccggttgca ggttatcgtt aagcatcacc acatcgctgg agacgcctac
    23521 tgcgctttcc ggcagatcgt gcaccacgat gcgcgcccaa gggacatcca cgcgaccatc
    23581 gagggtaaac aggtttggtg tagcctcgaa tacaacatct ggcgatacat ccattcgtac
    23641 catcggcggc acggtgatcc gcactttact gcctttcgcc gttacccgcg cccgccagtt
    23701 ttcaatttgg ctccagtcgg catcaccgtt caggtagatt tcaccctgct gggtccgtac
    23761 tgtaccggca agcgtcgagc gcataccgtt aaagttgacc gcaagctggc tcggttgcat
    23821 atcaaacggc ataaagttgc cgtcgatatc cacacccgta acctgaagct gaccaaacaa
    23881 ctgcgggctt tgcacatcac cacccagacg caagttggca ctcaccatcc ccgctgcttt
    23941 ttccccacgg gtaaagatgg ggtttatcat cgcaaggttg aagttacgga tattgacgtt
    24001 gccaccaaga ttacggcggc cttgcggatc ggtcacctgc acctgtccat caaactggcc
    24061 gttattggtc aggcggatgg tccagcccaa ttcggcacgg ttgttacgca attccgccgt
    24121 cagattcagc gtctgaaacg ccacgggcag cgccgcatcg ttgacggttt gcgttacctg
    24181 cacgttacgc cccgaaaggg tgatactgcc ctgcggcagc ccctctttcg tggtgtccca
    24241 ggcaacatcc gctttacccg tgaagatacc gctggcctga gtggtttctg gcataaacgg
    24301 tttcagcatg gcgaggtcga agcggttgag attcaccacc gcacgccctt cggcccccgc
    24361 atcgatagtt tgcggcacgc acagctccgc attcgggtta agccaacagt gtggcccgat
    24421 gctgattttt tgctccttat tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg
    24481 gccgaccggc gtctggaagc gggtattgct aagagttccc ttccagcgtt cttctttgcg
    24541 atcaaaactt cctgccagat taagctgccc ggagacaggc tcgccctgaa tccgcaactg
    24601 tagctcgtgc tgcttttcgc tgcctttggc attcagggtg acgaggttga tatttacatc
    24661 cggctgcgaa atttgctcaa cgcgtacgtc gagtttcccg gcgatctgat cggtggattt
    24721 aatgtcgcct tccacgcgaa cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc
    24781 ggtgatatct gccagtagtt gtggcgcttc caccgtgccg cgtactttca ccagcccttt
    24841 cgctgtaccg ccaagccccg gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc
    24901 aagattgaga tcttttaccc ccagctcgcc tttcacttcg gcactgtttg gccccagttc
    24961 cagatgaagc cctgggatca tccactgcat ataactgttg cctttcagcg tgccgtcaac
    25021 gttcactttg ttctgtttga cgttaccggt cagcttcagt tctggcacct ccatctgcca
    25081 ggtgccgccg tacaggctac cgcgggtttt aatcaagcca ttgagtttcg acggccactc
    25141 cgggaactct ttggcggtgt taatgccatt aagcgttagc tcaccgcgcc aactaatggc
    25201 ctgttgccag tcgagcaacg ccttgagttc agttttgcct tccagcgccg caacggtgag
    25261 tttgtcgaga ttgacctgct gttcattacc tttcgcgtcg agggtaatag tcgcgggcgg
    25321 gatctcctgt cccttcactg ccgtacgcat agagagcgtg taatcggtca ttttgccggt
    25381 aagtttcagt ttcagatcat ccgcctgata ctgcttctca ccagtgaacg gccagtaaag
    25441 ctgtttgctg ttcacttcca cgttgagcgg caatccagct tccgccagtc gcgtctgggc
    25501 gcgtaaatcc atatccaccg gaccggaaag attaacgcca atctccagct gttcgcgcag
    25561 cgcgccaccc acttttagct tcactttttc acctttcaac ggctccacgt tcagtgtgct
    25621 gttgagggtg atatccaccg gccagttgtc tgacagctgc gccgtgccgc tggcgttgac
    25681 gatcccctgg ttggaatcga tatccagggc gtccagttta gtattgccgt caatgctgct
    25741 cactttcagc agcatggtgc gcacggtgat gtccgtgtcg cccgtcacgc gtaactgctc
    25801 gcctttaaac tcttcaatgt tcaggttaag cggcaaatgc acgtcggtca tttccggcaa
    25861 taccgggcga gaaaagagat ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg
    25921 attttcaatt ttcggttcga ccacttcttc ctgcgccact tccgccactt tcggcagagc
    25981 aatcagcagg cctttcagcg acgtcggttt ca
//
LOCUS       DAEVZA010000066        25942 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEVZA010000066 DAEVZA010000000
VERSION     DAEVZA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.066"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..276
                     /locus_tag="LAF92_002711"
     CDS             <1..276
                     /locus_tag="LAF92_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI9791697.1"
                     /translation="EYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQV
                     CGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(316..1530)
                     /gene="ydcO"
                     /locus_tag="LAF92_002712"
     CDS             complement(316..1530)
                     /gene="ydcO"
                     /locus_tag="LAF92_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HBI9791698.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1583..2119
                     /gene="sutR"
                     /locus_tag="LAF92_002713"
     CDS             1583..2119
                     /gene="sutR"
                     /locus_tag="LAF92_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HBI9791699.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2192..4153
                     /gene="rlhA"
                     /locus_tag="LAF92_002714"
     CDS             2192..4153
                     /gene="rlhA"
                     /locus_tag="LAF92_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBI9791700.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4245..4475)
                     /locus_tag="LAF92_002715"
     CDS             complement(4245..4475)
                     /locus_tag="LAF92_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HBI9791701.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4697..4851
                     /locus_tag="LAF92_002716"
                     /pseudo
     CDS             4697..4851
                     /locus_tag="LAF92_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4897..5313
                     /gene="hicB"
                     /locus_tag="LAF92_002717"
     CDS             4897..5313
                     /gene="hicB"
                     /locus_tag="LAF92_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HBI9791702.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5392..6798
                     /locus_tag="LAF92_002718"
     CDS             5392..6798
                     /locus_tag="LAF92_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBI9791703.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7043..8188
                     /locus_tag="LAF92_002719"
     CDS             7043..8188
                     /locus_tag="LAF92_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI9791704.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8206..9219
                     /locus_tag="LAF92_002720"
     CDS             8206..9219
                     /locus_tag="LAF92_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI9791705.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9220..10161
                     /locus_tag="LAF92_002721"
     CDS             9220..10161
                     /locus_tag="LAF92_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9791706.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10151..10945
                     /locus_tag="LAF92_002722"
     CDS             10151..10945
                     /locus_tag="LAF92_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9791707.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10967..12391
                     /gene="patD"
                     /locus_tag="LAF92_002723"
     CDS             10967..12391
                     /gene="patD"
                     /locus_tag="LAF92_002723"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBI9791708.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            12601..13410
                     /locus_tag="LAF92_002724"
     CDS             12601..13410
                     /locus_tag="LAF92_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBI9791709.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
     gene            complement(13812..13907)
                     /gene="yncL"
                     /locus_tag="LAF92_002725"
     CDS             complement(13812..13907)
                     /gene="yncL"
                     /locus_tag="LAF92_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBI9791710.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            14102..14275
                     /gene="ortT"
                     /locus_tag="LAF92_002726"
     CDS             14102..14275
                     /gene="ortT"
                     /locus_tag="LAF92_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBI9791711.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            14361..14594
                     /locus_tag="LAF92_002727"
     CDS             14361..14594
                     /locus_tag="LAF92_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBI9791712.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(14595..15044)
                     /locus_tag="LAF92_002728"
     CDS             complement(14595..15044)
                     /locus_tag="LAF92_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI9791713.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(15041..15559)
                     /gene="mddA"
                     /locus_tag="LAF92_002729"
     CDS             complement(15041..15559)
                     /gene="mddA"
                     /locus_tag="LAF92_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBI9791714.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            15740..16777
                     /gene="curA"
                     /locus_tag="LAF92_002730"
     CDS             15740..16777
                     /gene="curA"
                     /locus_tag="LAF92_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBI9791715.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKKDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            16975..17640
                     /gene="mcbR"
                     /locus_tag="LAF92_002731"
     CDS             16975..17640
                     /gene="mcbR"
                     /locus_tag="LAF92_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBI9791716.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIDILYQQ
                     YNR"
     gene            complement(17676..19778)
                     /locus_tag="LAF92_002732"
     CDS             complement(17676..19778)
                     /locus_tag="LAF92_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBI9791717.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGIPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKQYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            20020..21081
                     /locus_tag="LAF92_002733"
     CDS             20020..21081
                     /locus_tag="LAF92_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBI9791718.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(21196..22695)
                     /gene="ansP"
                     /locus_tag="LAF92_002734"
     CDS             complement(21196..22695)
                     /gene="ansP"
                     /locus_tag="LAF92_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HBI9791719.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            22962..23579
                     /locus_tag="LAF92_002735"
     CDS             22962..23579
                     /locus_tag="LAF92_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBI9791720.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            23655..23867
                     /locus_tag="LAF92_002736"
     CDS             23655..23867
                     /locus_tag="LAF92_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HBI9791721.1"
                     /translation="MLCFLIYITLPFIQLVYFIFSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININTNKNIRQVC"
     gene            24688..>25942
                     /gene="tssI"
                     /locus_tag="LAF92_002737"
     CDS             24688..>25942
                     /gene="tssI"
                     /locus_tag="LAF92_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI9791722.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQLLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTG"
BASE COUNT         6698 a         6204 c         6618 g         6422 t
ORIGIN      
        1 gagtacaagc agctctggcg tggcggtcag gtgcttgctg ttccgccagc gtatacaagc
       61 cagcgttgcg cgtactgtgg tcatacagcg aaagagaacc gcctgtcaca aagtaaattc
      121 agatgccagg tatgtggata tacagcgaac gccgatgtaa atggcgctcg taacatttta
      181 gcggcggggc acgccgttct tgcctgtgga gagatggtgc agtcaggccg cccgttgaag
      241 caggaaccca ccgaaatgat tcaggcgaca gcctgaacgt agcagggatc cacgtccttc
      301 agggcgtgga ggatgtcaat atcggtttct gtcagcgatt aaattcaaca ccacgtaaca
      361 aacgcctccg gcaattaatc cccagaacgc agaaccaatc ccgaccagcg tcaatccact
      421 tgccgttacc agaaatgcca ccaccgccgc gtcgcgctca cgctcattat gcagcgcctg
      481 atacaaactg ccgccaatgg tactcaacag cgccagacct gccagcatct ggatccaact
      541 tacgggcagg gcagccatca tcccggtaat ggcactacca aacagacctg cgagcaaata
      601 gaaaatgcct gcaacggcag ctgccagcca acgttgatct ttatccggat gcgcttccgg
      661 gctttggcaa atagccgcgg tgattgccgc aataccgacg gaataaacgc cgaaagggga
      721 aaaaaccagt gccagcaatc cagtaaatac aattaatggc gaaacaggag ccgaatatcc
      781 agctgctttc attgctgcga tacccggtgc gttttgcgat gccatcgtca ccagaaaaag
      841 ggggagtgca acgctcaggc tgtgagcaaa cgaaaaatca ggggtaatat aagtggggag
      901 aacgggttta aagacaacat cagttgtgac aacgtcacct tgcgcgatga cgatcacgat
      961 cccaataatc atcgcggcaa ttaccgcata gcgcggcgca acggccttgg ttgccagcca
     1021 taccagcaac atacttccac acaacgtaaa ttgaccgtcc agactggcaa acgcctgtaa
     1081 accaaagcgt aataaaatcc cggcaagcat tgccgccgca agcgagtgcg gaataatgcg
     1141 catcagacga gcaaagagtc ccgttatgcc gcagaggact attaacgcgt tggtgacaat
     1201 aaaaacgccg atggcttcgt taagtgttag tccctgcaat ccggtgacca acaaagccgc
     1261 gccaggcgtt gaccatgcgg tgagaacagg tacgcgatac cataatgtca gagtcagcgt
     1321 actgacgccc attgccagcc ccagcgccgt catccagcca gagatttgtg cagtggtggc
     1381 tccggcgaca atcgctgctt gccagattat tgccgctgaa ctggcgtagc caattaatac
     1441 cgccagaaac cccgccagta gcgtgggtgg aggaatagaa aacagacgca tagtcacctc
     1501 gtgcgttata gcgtacaggc accgtagcat ttgtccgtta taacgcacaa gtgataaact
     1561 tccgttttgc cggaggagtc gcatggaaaa tctcgctcgc tttttatcca ccacacttaa
     1621 acaactacgc cagcagcgcg gctggagtct ttcgcgactg gcagaagcga cgggcgtttc
     1681 taaagcaatg ttagggcaga ttgagcgtaa tgagtccagt ccgacggtcg cgacattatg
     1741 gaaaattgcc accgggttga acgtaccatt ttcaacattt atttctccgc cgcagtccgc
     1801 tacgccttca gtttatgatc cacaacagca ggcgatggtg atcacttcgc tgtttcctta
     1861 tgatccacaa ctctgcttcg aacacttctc gattcagatg gcatctggtg caataagcga
     1921 atcgacgccc catgagaagg gggtaatcga acatgtcgtc gtcattgatg gacaacttga
     1981 tctgtgcgtt gatggggaat ggcagaccct taattgtggg gaaggcgttc gatttgctgc
     2041 agacgtcacg catatctatc gtaatggtgg ggagcaaacc gtacattttc attccctcat
     2101 ccattacccg cgcagttaag cgggaaaact atttcgcaac gtcgcgcttg tggctaaaat
     2161 agccgccatt tttcagctac tggataagaa tgtgaccgta tcttctcatc gacttgaact
     2221 gttaagcccg gcacgcgatg ccgccattgc ccgcgaagct attttgcacg gtgccgatgc
     2281 tgtttatatc ggcggccctg gttttggtgc ccgtcataat gccagtaata gcctgaaaga
     2341 tattgccgag ctggtgccgt ttgcccatcg ttatggtgca aaaattttcg tcacgcttaa
     2401 caccattttg catgatgatg agctggaacc cgcgcaacgg ctgattactg acctctacca
     2461 gaccggtgtc gatgcgctga ttgttcagga tatggggatt ctggaacttg atattccgcc
     2521 gattgaactg cacgccagta cgcagtgcga cattcgtaca gttgaaaaag cgaagttcct
     2581 ctctgatgtt ggcttcacgc agattgtgct ggcgcgagag ctgaatcttg agcagatccg
     2641 cgcgattcac caggccacgg acgcgaccat tgaattcttt attcatgggg cactgtgcgt
     2701 ggcctattcg ggtcagtgct acatttctca tgcgcaaaca gggcgtagcg ccaaccgtgg
     2761 tgattgctcg caggcgtgcc gtttgccata cacattgaaa gacgatcagg ggcgggtggt
     2821 ttcctatgaa aaacatctgc tgtcgatgaa agataacgat cagactgcca acctcggcgc
     2881 gctgattgat gctggcgtac gctccttcaa gattgaaggg cgttacaaag atatgagcta
     2941 cgtgaagaat atcaccgccc attatcgcca gatgcttgat gccattattg aagaacgtgg
     3001 cgatctggcg cgcgcttcat caggtcgtac tgaacatttc tttgttccat cgacggaaaa
     3061 gactttccac cgtggtagca cagattattt tgtgaatgcc cgtaaaggcg atattggcgc
     3121 gttcgattcg ccgaaattta tcggcctgcc ggtaggcgaa gtattgaaag tggcgaaaga
     3181 tcatctcgat gttgccgtca ccgagccact ggcaaatggt gatggcctga acgtgatgat
     3241 taaacgtgaa gtcgtcggtt ttcgtgccaa tacggtcgag aaaaccggag aaaatcagta
     3301 ccgcgtctgg cccaatgaaa tgccagcaga tttgcacaaa attcgtccac atcacccact
     3361 aaaccgtaat cttgatcata actggcagca ggcactgaca aaaacctcca gcgaacgtcg
     3421 ggtggcggta gacattgaac tgggcggctg gcaggaacaa ctgattctga ccctcaccag
     3481 tgaagagggt gtcagcatca cgcatacgct ggacgggcag ttcgacgaag ccaataacgc
     3541 cgaaaaagca atgaacaatc tgaaggatgg tctggcaaaa ctggggcaaa ccctctatta
     3601 cgcccgcgat gtgcaaatta atttgccggg ggcgctgttt gtaccaaaca gtctgttaaa
     3661 ccagttccgc cgtgaagctg ctgacatgct ggatgctgcg cgtcttgcca gttaccagcg
     3721 cggcagccgt aaaccggttg ctgatcctgc gccggtttat ccgcaaacgc atctgagttt
     3781 cctcgcgaac gtatacaacc agaaagcgcg tgaattttat catcgctatg gtgtgcagct
     3841 aattgacgcg gcgtatgaag cacatgaaga gaagggcgaa gtcccggtga tgatcaccaa
     3901 gcattgtctg cgctttgcct ttaatctgtg cccgaaacag gcgaaaggca atatcaaaag
     3961 ctggaaggcg acgccaatgc aactggttaa cggcgatgaa gtattaacgc taaagtttga
     4021 ttgccgccca tgcgagatgc acgtcattgg caaaatcaaa aatcacatac tgaaaatgcc
     4081 gttaccggga agcgtagtgg catccgtaag tccggatgag ctgctgaaaa cattgcctaa
     4141 gcgaaaaggg taaaacgcca gttttctggt tactcacaac tcattgaatc tgcatgatat
     4201 tgcctgccgg gtaaggcgtc tcgccgcatc cggcaacaaa tgactcaacg ttgtcccgct
     4261 tctggtttgc gcgatttttg ccagtaaaaa tgctcgcgca aaccttccgc cgactcttcc
     4321 gccacagcac gcaattcatc gctgtccgct tcatgacgca gctgatgatc cacattcttt
     4381 acccacacaa attcatgtcc tttgtgccct gccatgagtt gtcctgagaa cagcgcacac
     4441 gttaataaga caaccgataa cgccttcgta aacatcctgc cacctttttc ttaccttttg
     4501 ccgctatgat gccgatcgtt tcttgaggtt attattcagt tttgcaaatt agcgcaaaga
     4561 aattctggaa tcttccttcc tgattttgca tggcattctg ccgttgcggc gatttagtgc
     4621 tattttcgag caaattacac acggaggtaa acaataatgt ttactttgtt gatatactca
     4681 gcggcaggga ggcgatgtga aacaaagcga gttcagacgt tggctcgaat ctcagggcgt
     4741 cgatgtagcg aatggccgca accatttgaa actcaggttt catgggaggc gcagtgtcat
     4801 gccgcgtcac tcctgcgtaa agcaatcctg aaacaactcg gtttgagtta atcgccaatt
     4861 aaaaaggtta atgacatgcg agagacagtc gaaattatgc gttatcccgt cactcttaca
     4921 cccgcgccgg aaggcggtta tatggtttct tttgtggata tccctgaagc gttgacgcag
     4981 ggcgaaactg tcgctgaagc gatggaagcg gcaaaagatg ctttactgac cgcatttgat
     5041 ttttattttg aagataacga gcttatcccg ttaccttcgc cattaaatag tcatgatcac
     5101 tttattgaag tacctttgag cgtcgcctct aaggtattgc tgttaaatgc ttttttacag
     5161 tcagaaatca ctcagcaaga gttagccagg cgaattggca aacctaaaca ggagattact
     5221 cgcctattta acttgcacca tgcgacaaaa atcgacgccg tccagcttgc ggcaaaggcg
     5281 cttggcaaag agttatcgct ggtgatggtt taattacagt taacgaaaag ttgtcatttt
     5341 taacaactga tatagactgc cgaatcatct gcacataatt acgattcgat aatgaaaaaa
     5401 taccagcagc ttgcagaaca attacgcgag cagattgcgt cgggtatctg gcaacccggc
     5461 gatcgtttgc cttcgttgcg tgaccaggtg gcgctttcag gcatgagctt tatgactgtc
     5521 agccatgcct atcagttgct cgaaagtcag ggatatatta tcgcacgacc gcagtcgggt
     5581 tattacgttg cgccacaggc aataaaaatg ccgaaagcgc cagtcattcc agtcactcga
     5641 gatgaagcag tcgatatcaa cacttatatt tttgatatgt tgcaggccag tcgcgatccg
     5701 tcggtcgttc cgtttgcctc ggcctttccc gacccgcgac ttttccccct ccaacaacta
     5761 aaccgctcgc tggcgcaggt aagcaaaacc gccacggcga tgagcgtgat tgaaaactta
     5821 ccgccaggaa acgcagaact gcgtcaggct attgctcgtc gctatgcctt acagggcatc
     5881 accatttctc ctgatgaaat tgtcattact gccggggcgt tagaggcatt aaacctcagt
     5941 ttgcaagcgg taactgaacc gggcgattgg gtgatagtag agaatccttg tttctacggt
     6001 gcgttgcagg cgctggagcg gctacggctg aaggcgttat cggtggcgac ggatgttaaa
     6061 gaagggatcg atcttcaggc gctggaactg gcgttgcagg attatccggt gaaagcgtgc
     6121 tggctgatga ctaatagcca gaacccactc ggatttacct taacgccgca aaaaaaagca
     6181 caactggtgg cgttactcaa tcagtacaac gtaacgctga ttgaagatga cgtttacagc
     6241 gaactttatt ttggacggga aaaaccgctg cctgcgaaag cgtgggatcg ccacgatggc
     6301 gttttgcatt gctcttcgtt ttcgaaatgt ctggtgcctg gttttcgtat tggttgggtc
     6361 gccgccggaa aacatgcacg taaaattcaa cgcttgcagt tgatgagtac gctttccacc
     6421 agctcaccga tgcaacttgc gctggtggat tacctttcca cgcgccgata cgacgcccat
     6481 cttcgtcgcc tgcgtcgcca gcttgcggaa cgtaaacaac gtgcctggca ggcactgctg
     6541 cgttatctgc ctgcggaagt taaaattcat catagtgaca gcggttattt tctctggctg
     6601 gagctccccg agccgttaga tgccggcgaa ttaagcctgg cggcactgac gcatcatatc
     6661 agtattgcgc cgggtaaaat gttttctacc ggtgaaaact ggtcacgttt ttttcgtttt
     6721 aataccgcgt ggcagtgggg agaacgtgaa gaacaggcgg taaaacaatt aggcaaactt
     6781 attcaagaac ggctgtaata gcgtttaatt taattcctct tagattgggt aatatgaatt
     6841 tcgaatagca gtcatatttc ctaacccctt gtctatactc cagaagataa ccttacagac
     6901 ggcataatgc gcggtagctc acaacctgat taaattttct caggggcgaa ggtgtgcctg
     6961 caagccgccg tctatggtta aacaaggaga tatttttacg gcacggcggc tgaacaatta
     7021 attacgacag gagtaagacc ttatgagcaa gacatttgcc cgcagcagcc tgtgtgcgct
     7081 caccatgaca ataatgaccg ctcacgccgc cgaaccacct accaatttag ataaaccgga
     7141 agggcgactg gatattatcg cctggccggg atacatcgaa cgcggacaaa ctgataaaca
     7201 atacgactgg gtaacgcaat ttgaaaaaga gacaggctgc gcggtgaatg tgaaaaccgc
     7261 cgcgacttcc gatgaaatgg tcagtctgat gaccaaaggg ggttacgatc tggttacggc
     7321 atccggcgat gcctcgctgc gtttgattat gggcaaacgc gtgcagccga ttaataccgc
     7381 cttgattccc aactggaaaa cgctcgatcc gcgcgtggtt aaaggcgact ggtttaatgt
     7441 tggcggcaaa gtttacggca caccttacca atgggggccg aacctgctga tgtacaacac
     7501 taaaaccttc ccgacgccgc cggatagctg gcaagtggtt tttgttgagc aaaatctgcc
     7561 ggacggcaag agcaataaag gccgcgttca ggcttatgat ggccctatct acattgcgga
     7621 cgctgcgttg ttcgttaaag ccactcagcc gcagttgggc atcagcgatc cgtatcaact
     7681 aaccgaagaa cagtaccagg cggtgctgaa agtgctgcgc gctcaacaca gtttgatcca
     7741 tcgctactgg catgacacta ccgtgcaaat gagcgatttc aaaaacgagg gtgtggttgc
     7801 ttccagtgcc tggccctatc aggccaacgc cctgaaagcc gaaggccagc ctgttgctac
     7861 cgttttcccg aaggagggtg ttaccggttg ggctgatacc accatgctgc atagcgaagc
     7921 gaaacatccg gtttgcgcct acaaatggat gaactggtca ttaacgccaa aagtgcaggg
     7981 cgatgtggcg gcctggtttg gctcgttacc ggtagtgccg gaagggtgta aagccagtcc
     8041 gttattaggc gaaaaaggtt gtgaaaccaa cggttttaac tatttcgaca aaatcgcctt
     8101 ctggaaaacg cctatagcag aagggggcaa gtttgttccc tacagtcgct ggacgcagga
     8161 ttacattgcc attatgggcg gtcgctaact tcgctggggt gctttatgac gtacgcagtg
     8221 gagtttgaca acgtctcgcg gttgtacggt gacgtgcgcg cagtagatgg cgtcagtatt
     8281 gcgataaaag atggtgagtt cttctctatg ctggggccgt ccggctccgg caaaaccacc
     8341 tgcctgcgcc tgattgctgg cttcgaacag ctttccggcg gggctatctc tatctttggt
     8401 aaacccgcca gcaatctgcc accgtgggag cgggacgtga atactgtctt tcaggactac
     8461 gcgctatttc cgcatatgtc gattcttgac aatgtcgcct atgggctgat ggtcaaaggc
     8521 gtgaataaaa agcagcggca cgcaatggcg caagaggcgc tggagaaagt ggcgttgggg
     8581 tttgtacatc aacgtaaacc gtcacaactt tctggtggtc agcgccagcg ggttgctatc
     8641 gccagagcat tggtgaatga accgcgcgta ttgctgttgg atgaaccgct cggcgcactg
     8701 gatctcaaat tgcgtgagca gatgcagctg gaactgaaaa aactgcaaca gtctctcggt
     8761 atcactttta tcttcgttac ccacgatcag ggcgaagcgt tatcgatgtc cgatcgtgtg
     8821 gcggttttca ataatggacg cattgagcag gtcgattccc cgcgcgatct ctatatgcgc
     8881 ccgcgcacgc cgtttgttgc cggattcgtc gggacatcca acgtttttga tggactgatg
     8941 gcagagaaac tttgtggcat gacgggaagc ttcgccctgc gaccggaaca tatccgcctc
     9001 aacacccctg gtgaactgca ggccaatggc acgatccagg cggtgcaata tcagggcgcg
     9061 gcaactcgtt ttgaactgaa attgaacggc ggtgaaaaac tgcttgtgag tcaggccaat
     9121 atgacaggcg aagaactgcc tgccacgctc acgcccggac aacaggtgat ggtttcctgg
     9181 tcgcgtgatg tgatggtgcc gctggttgag gagaggtgaa tggcgatgaa tgtattgcaa
     9241 tcaccttcac gtccaggtct gggtaaggtg tccggtttct tctggcgtaa tccggggctg
     9301 gggctgtttt tactgctgct tggcccgcta atgtggtttg gcattgtcta tttcggctcg
     9361 ctgctgacac tgttatggca gggattttat acttttgacg atttcaccat gtcggtaacg
     9421 ccggaactga cgctggcgaa tatccgtgcg ctgtttaatc cggcgaatta cgacatcatt
     9481 ctccgcacgc tgactatggc tgtggcagtc actatcgcca gcgccattct ggcttttcca
     9541 atggcgtggt atatggcgcg ctataccagc gggaaaatga aagctttttt ttatattgcg
     9601 gtaatgttgc cgatgtgggc gagctacatt gttaaagcct atgcctggac gttattgctg
     9661 gcaaaagatg gcgtggctca gtggttttta caacatcttg ggctggaacc actgctgact
     9721 gcgttcctta cattacctgc ggtgggcgga aatacgctgt caacttccgg gctggggcgc
     9781 tttctggtgt ttctctatat ctggttgccg ttcatgatcc tgcccgttca ggcggcgctt
     9841 gagcgtttgc cgccgtcatt gttgcaggcg tcggctgatc tcggcgcacg tccacgacaa
     9901 acctttcgct atgtggtgct gccgctggca atcccgggta ttgccgctgg ctctatcttt
     9961 accttctcac tcacactggg cgattttatc gtcccgcagc tggttggtcc tccaggatat
    10021 tttatcggca atatggttta ttcccagcag ggggcgattg gcaatatgcc gatggcggcg
    10081 gcattcaccc tggtgccgat tgttctcatc gcactgtacc tggcgttcgt gaaacgtctg
    10141 ggagcgttcg atgcactctg aacgcgcacc gtttttcctc aaactggcgg cctggggcgg
    10201 cgtggttttc ctacattttc ccatcctgat aatcgccgcc tatgcgttta acactgaaga
    10261 tgcggcgttt agttttccac cgcagggcct gacgctgcgc tggtttagcg tggcagcaca
    10321 gcgtagtgat attcttgatg ccgtgacact gtcacttaaa gtggcggcgc tggcgacatt
    10381 aattgcgctg gtgttaggga cgctggcagc tgccgcgctg tggcgacgag actttttcgg
    10441 caaaaacgcc atttcgctgt tactgctgct gcccattgcg ctgccgggca ttgtcactgg
    10501 tctggcgtta ttaaccgcct ttaaaaccat caatctggag ccgggatttt tcaccatcgt
    10561 ggtcggtcat gcgacttttt gtgtagttgt ggtgtttaac aatgtcatcg cccgttttcg
    10621 ccgcacctcg tggagtctgg ttgaggcgtc aatggatctt ggggccaatg gctggcaaac
    10681 cttccgctac gtagtgttgc cgaatctcag ttcggcgtta ctggcaggag gaatgctggc
    10741 gtttgccttg tcgttcgatg aaatcatcgt tacgaccttt acggcaggtc atgaacgaac
    10801 gttaccgttg tggttgctca atcagcttgg gcgaccgcgt gatgtaccgg taaccaacgt
    10861 ggtggcactg ctggttatgt tggtaacaac cttgccgatc ctgggggcct ggtggctaac
    10921 ccgcgaaggc gacaatggtc aataaccact gatacaggaa tatgctatgc aacataagtt
    10981 actgattaac ggagaactgg ttagcggcga aggggaaaaa cagcctgtct ataatccggc
    11041 aacgggggac gttttactgg agattgccga ggcatccgca gagcaggtcg atgctgctgt
    11101 gcgcgcggca gatgcagcat ttgccgaatg ggggcaaacc acgccgaaag tgcgtgcgga
    11161 atgtctgctg aaactggctg atgttatcga agaaaatggt caggtttttg ccgaactgga
    11221 gtcccgtaat tgtggcaaac cgctgcatag tgcgttcaat gatgaaatcc cggcgattgt
    11281 cgatgttttt cgctttttcg cgggtgcggc gcgctgtctg aatggtctgg cggcaggtga
    11341 atatcttgaa ggtcatactt cgatgatccg tcgcgatccg ttgggggtcg tggcttctat
    11401 cgcaccgtgg aattatccgc tgatgatggc cgcgtggaaa ctggctccgg cgctggcggc
    11461 agggaactgc gtagtactta aaccatcaga aattaccccg ctgaccgcgt tgaagttggc
    11521 agagctggcg aaagatatct tcccagcagg cgtgattaac gtactgtttg gcagaggcaa
    11581 aacggtgggc gatccgctga ccggtcatcc caaagtgcgg atggtgtcgc tgacgggctc
    11641 tatcgccacc ggcgagcaca tcatcagcca taccgcgtcg tccattaagc gtactcatat
    11701 ggaacttggt ggcaaagcgc cagtgattgt ttttgatgat gcggatattg aagcagtggt
    11761 cgaaggtgta cgtacatttg gctattacaa tgctggacag gattgtactg cggcttgtcg
    11821 gatctacgcg caaaaaggca tttacgatac gctggtggaa aaactgggtg ctgcggtggc
    11881 aacgttaaaa tctggtgcgc cggatgacga gtctacggag cttggacctt taagctcgct
    11941 ggcgcatctc gaacgcgtca gcaaggcagt agaagaggcg aaagcgacag ggcacatcaa
    12001 agtgatcact ggcggtgaaa agcgcaaggg taatggctat tactatgcgc cgacgctgct
    12061 ggctggcgca ttacaggacg atgccatcgt gcaaaaagag gtatttggtc cagtagtgag
    12121 tgttacgccc ttcgacaacg aagaacaggt ggtgaactgg gcgaatgaca gccagtacgg
    12181 acttgcatct tcggtatgga cgaaagatgt gggcagggcg catcgcgtca gcgcacggct
    12241 gcaatatggt tgtacctggg tcaataccca tttcatgctg gtaagtgaaa tgccgcacgg
    12301 tgggcagaaa ctttctggtt acggcaagga tatgtcactt tatgggctgg aggattacac
    12361 cgtcgtccgc cacgtcatgg ttaaacatta agttagatgt tagtggctgt tataggacgg
    12421 ccactatcgt tttaggttct gagtttaatg gcggttaagt attagaatga ttacattaat
    12481 aactagcgta aaaattaggc taaatgtttg atggcgattt tttatcctgt cacagttgat
    12541 tagcataagg ctttagagta tcgttattaa actaataatt tataagtaga agtattaaca
    12601 atgaaagttc aattgtacaa attcacggaa gataaaaata aaacccttac cttcagatgg
    12661 acgaaaaaac actttgagtt ttgtatggat aataaaatct ttttaaatca taaggggaag
    12721 aagtcgtata aagaaaggaa tttatttctt ttctcaaaag gtgacaagat aacaattgaa
    12781 gataatgtta ttgctgaaga atattcaaca atgcctgtga aaaatttctc cagtgtcggc
    12841 gctttttcat ttccgacctg ccatttttca gggaatatac gtataggtcg attttgtagt
    12901 attgcatcaa atgtaaaaat aatgggtgga aatcatcctt tgaatcgttt tactactcac
    12961 atgatgactt ataacggtga atttgataag tttgctatga gtgaatttga acgtagctgg
    13021 acattgaaac cattcattac caaacctgaa aatccgatta taggaaatga tgtctggata
    13081 ggaaatgatg ttgtattgaa aggtggcatc gcaataggtg atggtgctgt tattgctgcc
    13141 aattccgttg ttaccaaaga tgtgccccca tatgccatag ttgcaggagt tcctgcaaaa
    13201 ataatcagat ttaggtttga ttcaaatgtt attgatgaac tgttgagaat aaaatggtgg
    13261 aattataact actcagatct tccggataat aacaagtgtg atgatataaa ttattttgtt
    13321 gaagagatga atcgattgat atcagatgga aatattcaag agagggacta taaaaaattc
    13381 aatctttctg aggtattcag aggcctataa ttatctgttt tgcatgataa atgggcaaga
    13441 taaccagggc ggggtttgca atttcaatgg ggcagcgtca tcaaggaggt tgcaaacatc
    13501 ctgccatgca tatacttttc gccatctttt tatatttacg ttattccttg aaagtataaa
    13561 gcatttgtag actcccgcgc gtataaagta ttcatgatag aaaaatgtga aaacaagatt
    13621 ccgtttgaat cagtaagctg tttgtataga cgacagtagg gggatgtttt ctactcattt
    13681 caatgtagat gccagcaaaa agaagtaaat attaaggata atgttgtaaa cctggagagt
    13741 atgagaagat cttgcgtact gtcagagagt tgatttctct gacagtacgt gctgcgtgca
    13801 aaatcactac atcaaataaa ccagccaaat ctcatagaga taagagtaaa caaaccaaca
    13861 gcagcaaaga catttatcag tactacagtt ctactggaaa cattcatttt tgccaccttt
    13921 tatcattgcc cctatgtgta tagtacgggt taagaaaatc cgcaataata ctcattctga
    13981 agatgaataa gggcaagttg ctgtttgatg attttttctc aaactggtga aaaaacttga
    14041 tgcacgtcaa aaaatgacgc atatttgcgc gcgttttatt catctggctg gacgcccgta
    14101 catgtctctc tatcaacata tgcttgtttt ttatgcggtt atggcagcaa tcgcatttct
    14161 tatcacctgg tttctttctc acgataagaa acgcatccgt ttcttaagcg cctttctggt
    14221 gggggcaaca tggccgatga gtttcccggt ggcgctgttg ttttcactgt tttaaatctt
    14281 actcactggc ttgacggcac cacgcagact tatatcattt ggatgaatcg ataaatttca
    14341 caagtggtta aggagaaagt atgtcgcatc tggatgaagt catcgcgcgc gtggatgccg
    14401 cgattgaaga gagcgtcatt gcccatatga acgaattatt aatcgccctg agcgatgacg
    14461 cggagttaag tcgggaagat cgctacaccc agcagcagcg tttgcgcacc gctattgctc
    14521 atcacggtcg caagcataaa gaagatatgg aagcgcgcca cgaacagtta accaaaggcg
    14581 gcaccatcct ctgattaaaa tgagcgtctg gctaccaacc aggcgccaac cacaagtaaa
    14641 attgcgccac aaattgggcc gaacaacgcc cgtagtggta cgccatgact gcggaagata
    14701 tccagcatca gaccgccaat tagctggctg gcgaccagta ctgcaatagt tgtcgccgct
    14761 cccacattct gatatccgct gatactggcg aagacaaaaa acgatcccag caagccgggt
    14821 ataagtgtcc accagcgcac gctggacacc agttcgccaa accccgccat gccttgttta
    14881 aaccataaaa tagaaacaaa gagcacaatc cccaccagtg agttcagcaa catggcgatg
    14941 agaatggtag aggaggtctg ggtgatgcgc accatcagcg tgttctggac taccagacca
    15001 attccggcgg caatcaaaaa agcaagggtg agcgactgat tcatccaatc gcgtccggtt
    15061 cagtgcgttc gtcgagttgc aactgcataa atgtcagatc cagccagcgc ccaaatttag
    15121 tgcccacctg cggcatttgt gcggtgacga caaatcccag cgactggtgc agatgcagcg
    15181 aggcctgatt ttgcgattcg atcccggcga ccatgacatg cttcccgcaa tcccgcgctt
    15241 catcaatcaa tcggcttaac aatttacgcc ccagaccttt gccctgatga tcgggatgga
    15301 cataaaccga atgttccacg gtatggcgaa aaccatcgaa actacgccag tcgccaaacg
    15361 aggcatatcc cgtcactacg ccatcttctt cactcaccag caccggataa cctgctatgg
    15421 tccgcgcttc aaaccaggca atgcggttat ctgcatcaac cgtttggtca ttccagatag
    15481 ccgccgtata caacacggcg tggttataaa tttccgcaat ggcagcacaa tcggctttgc
    15541 gggcaaaacg gatggacatg tctggcctcg ataaaataat gatgatgatg ttatcaatac
    15601 tatcgaaaac agcccgaagc aacggattcc tcgatgaaat tattgcgtgt tgcagagggg
    15661 atgtgacggc tgcaaacaaa accaatcccg tgagcctgct actattgatg gattcatgcc
    15721 aaaagcacag gagagcatta tggggcaaca aaagcagcgt aatcgacgtt gggttctggc
    15781 ctcgcgtcca catggcgcac cggttccgga gaatttccgt cttgaagaag atgatgtcgc
    15841 cacaccgggt gaaggacagg tgttactgcg cacagtttat ttgtccctgg acccgtatat
    15901 gcgtggacgt atgagcgatg agccatctta ttcaccgcct gttgatattg gcggcgtgat
    15961 ggtcggcggc acggtgagcc gtgttgtgga gtcgaatcat cctgattatc agcctggcga
    16021 ctgggtgctg ggctacagtg gatggcagga ctatgacata tccagtggtg atgatctggt
    16081 gaaacttggc gatcatccgc aaaatccatc gtggtcgctg ggtgtgctgg ggatgccagg
    16141 ctttaccgct tatatggggc tgctggatat cggtcagcct aaagagggcg aaacgttggt
    16201 ggtagctgcg gcgacaggtc ctgtgggagc aacggtgggg caaatcggca aacttaaagg
    16261 ttgcagggtg gtgggggttg ccggtggcgc ggaaaaatgc cgccatgcta tcgaggtgct
    16321 gggcttcgat gtttgtcttg atcaccacgc ggatgatttt gccgaacaac tggtgaaagc
    16381 gtgcccaaaa ggtattgata tctattatga aaacgtgggc ggtaaggtat ttgatgcagt
    16441 gctaccatta cttaatacat ctgcgcgcat tcccgtttgt ggcttagtga gcagctataa
    16501 cgctacagag ctaccacccg gtccggatcg tttacccctg ttgatggcaa cggtgctgaa
    16561 aaaacgtatt cgcttgcaag ggtttattat cgctcaggat tatggtcacc gcatccatga
    16621 gtttcagaag gagatggggc aatgggtgaa aaaggataaa atccactacc acgaagatat
    16681 tactgatggt ttagagaatg cgccacagac gtttatcggc ctgctgaagg gtaaaaactt
    16741 cggcaaagtg gtgatccgcg tcgcgggtga tgattaaagt ttcaacggcg gcgtaagccg
    16801 ccataaaaat ccaggcaaaa agcttatttt cagctttaat taactaacca cgtcattgtt
    16861 ctgctgctaa taacaacaaa ggtgggtggt ttatgattat tcactttaat acaccaggtg
    16921 aactccttct gccgtgcagg cagggttgga cagaaaacac gcgaaggaat taaaatgcct
    16981 ggaatgggaa aaatgaaaca tgtcagtttg actctgcagg ttgagaacga cctgaaacat
    17041 cagcttagta ttggcgcatt aaaacctggc gcacgcctga ttactaaaaa tctggcggag
    17101 caattaggta tgagtattac acctgtgcgt gaagcattat tacgtctggt ttcggtgaat
    17161 gcgctttctg tcgcacctgc acaagcattt acagttccgg aagtggggaa acgtcaatta
    17221 gacgaaatca atcggatccg ctacgaactg gaattaatgg cagttgctct ggctgttgaa
    17281 aacctcaccc cgcaagacct tgcggaactc caggaattgc tcgagaaatt acagcaggcg
    17341 caagaaaagg gcgacatgga acaaatcatt aatgtaaaca ggctatttcg cttagcgatt
    17401 tatcatcgct caaatatgcc catcctgtgt gagatgattg agcaactgtg ggtcaggatg
    17461 gggcctggtt tacattatct ctatgaagcg attaatccag cggaattacg ggagcatata
    17521 gaaaactatc atctgttact cgctgcgtta aaagcaaaag acaaagaggg atgcagacat
    17581 tgtcttgctg aaattatgca acaaaatatt gatattttat accagcaata caatcgttaa
    17641 agtaaatgcc cggcaaatac cgggcatcaa ggtaattact caaatctcca cgcaatattc
    17701 atgccgacac cataatttcg tccgggcgaa ggttcgtaat atcgcccgtt tgactcattg
    17761 acaatgacag aaccaacgta ttgtttatcg aataaattat cgacacgacc aaataaatcc
    17821 acagttaaat tgtggtaatt gtatttatac ccggtgaata agccgacgag agtataagac
    17881 ggcgcttttg ccgtattttc atcatctgcc ataatatcgc ccatataacg cgcttccgtg
    17941 cctgcatacc aaccatcttc cggtacataa cctatcgacg caaagcccat attacgggcg
    18001 atccctggca tccgattacc gttacaatcc tgttcattgc aaacattgct gcgataggtc
    18061 gcatcaagcc aggtccagga cgcgtttacg cgaaaatctc ctgcgaaacg ttgatcccat
    18121 gccagttcag cgccttgacg acgggtcttt ccggcatttt tgtaagtcgt acgcccaccg
    18181 ctactgctat cgacaacaat ttcatcatca gtgtcggtct gaaacaatgc gagactaagc
    18241 agcccatcac caatacgcgt tttactgccg atctcaattg tatcgttggt ggatggtttt
    18301 aaaccaaagt tcataccgct ttgcccatca gcacgataag acagctcatt aatcgtcggc
    18361 gtttcaaaac ctcgcccggc tgccagatag atattccagg catcggtcat tgcatatttt
    18421 aacgaaccgg caggtagcca tttatgataa ctggcatcac cgctgtcatc gccgttaccc
    18481 ggagtaacgt aatggtcgtt ggaatcaaac cacacggagc tgtagcgcac gccagcatcc
    18541 agcgacagtt tttcgctcag ctgccactgc gtctgtaaat agggatcgat gttccacatc
    18601 agattgcgtt cgtcgcgacg caactcacct ttttgcccgt actccggtat gccgctattc
    18661 aggcggaagt tattgtagcc cttgcggttt tcactcatgt tttcgtagtt caggccggta
    18721 gtgaacgtga ccggaacgcc cagttcacca cggtgtgtcc agcggctgtc tatcccctgg
    18781 taatggcgtt gcagggtaat cacgccgccc gcatgtgacg ggttaagttg gggggccatc
    18841 gggattgact ggtactgggt cgtttctcgc tctccggcat acatcatcac actcatatca
    18901 tcttgcgcgc tcaggctacg ctcatagcgc aacccagcct gagtttgctt gatggttttt
    18961 cgcgtgtcgt actgttctgc acgaggcgct tgttgtggat tagccttcca ttctgctttg
    19021 gttagcccac cagggtcatc tgctttgata tccacactat tgaaaatcag acttaatttg
    19081 ctggcttcat caatgcgtac gcccagtttg gcattagcta aatttttctg tgcgccacta
    19141 tggtcacgat agccgtgggt cgtaaaacgc gtggttgaga cggtgtaatc gacatcgcca
    19201 ggctgtgtgc cgtctcccgt tgcgcccgtt gctttcagcc catagcgcca gctgccaaaa
    19261 ctgccgtagt aactactggc ttcaatggtt ggtggctgtt gtccggtctg ggtggtgaca
    19321 ttcattaccc caccagacgc gttgccatac agggcagaga aggggccacg cagcacttcc
    19381 acattttgca cactgcttaa atcgatgttg gatgtttgcc cttgcccgtc gggcatggtg
    19441 gcgggaatac cgtccacata caggcgaata ccgcgaatac cgtaagtgga gcgggagcca
    19501 aatccgcgaa tcgacagctg taaatcttgc gcatagttct gccggttttg tacctgcaaa
    19561 ccaggcacgc cggtcagtga ttcggacaag ttaatgcgcg gtgttgccag gcgcatctcc
    19621 tcgccatcca ccacgcttac tgctgctggg gtatccagtt ctgaaaccac ctgcggtgcg
    19681 gcactgacaa tcatagtctg ttcatcagcg gcaaaaacaa cgggggaaag gacaagcagt
    19741 gcgggcaaaa cggtctgtcg gacggaaaaa atcttcatga aaaaagccag gttaagaatg
    19801 ggaaaacgcc gttatggtaa tgaaattgta aatttatgga aaacgaaacg gcacgatacg
    19861 ttaagtaatt gagaaaattg tagtcgtaac ggcaagaaat gctccacatt tgagaaaata
    19921 atgattacca ttcccattta taacaagagc gtaacgatga ttacgcttag cgaagcattg
    19981 tgaagcagca aaaatatcgg ttcatcaaag ggagtcgtca tgcatttacg tcatctgttt
    20041 tcatcgcgcc tgcgtggttc attactgtta ggttcattgc ttgttgcttc atcattcagt
    20101 acgcaggccg cagaagaaat gctgcgtaaa gcggtaggta aaggtgccta cgaaatggct
    20161 tatagccagc aagaaaacgc actgtggctc gccacttcgc aaagccgcaa actggataaa
    20221 ggtggcgtgg tttatcgtct tgatccggtc actctggaag tgacgcaggc gatccataac
    20281 gatctcaagc cgtttggtgc caccatcaat aacacgactc agacgttgtg gtttggtaac
    20341 accgtaaaca gcgcggtcac ggcgatagat gccaaaacgg gcgaggtgaa aggccgtctg
    20401 gtgctggatg atcgtaagcg cacggaagag gtgcgcccgc tgcaaccgcg tgagctggta
    20461 gctgatgatg ccacgaacac cgtttacatc agtggtattg gtaaagagag cgtgatttgg
    20521 gtcgttgatg gcgagaatat caaactgaaa accgccatcc agaacaccgg taaaatgagt
    20581 accggtctgg cgctggatag caaaggcaaa cgtctttaca ccactaacgc tgacggcgaa
    20641 ttgattacca tcgacaccgc cgacaataaa atcctcagcc gtaaaaagct gctggatgac
    20701 ggcaaagagc acttctttat caacattagc cttgataccg ccaggcagcg tgcatttatc
    20761 accgattcta aagccgcaga agtgttagtg gtcgataccc gtaatggcaa tattctggcg
    20821 aaggttgcgg caccagaatc actggctgtg ctgtttaacc cagcgcgtaa tgaagcctac
    20881 gtaacgcatc gtcaggcagg taaagtcagt gtgattgacg cgaaaagcta taaagtggtg
    20941 aaaacgttcg atacgccgac tcatccaaac agcctggcgc tgtctgccga tggcaaaacg
    21001 ctgtatgtca gtgtgaaaca aaaatccact aaacagcagg aagctaccca gccggacgat
    21061 gtgattcgta ttgcgctgta atagatgtgg cgagggtgaa aacggagaga cttgcctgac
    21121 ttttatcacg ccgcatgcgg cacaccttcg tcagtaatct aaaattgccc ggcacgtagc
    21181 cgggcaattt tgcaattagc tcgccgtttc aggcttaaac acaatttcct gtttttcttc
    21241 atcttcctcg acgactggcg cagtgctgtg aatttcagca acgcgtttgc gcacaccaaa
    21301 ccagcctata accagcagaa taccgataat cggcagcgcc gcgatagtat aagtcccgtt
    21361 cgggtaatcg aacgccatca gcacaaggac actcagtaaa aacagtaatg tcagccagga
    21421 agtgaagggc gcgccaggca gtttaaaact gacatccgct gctttgcctt gtttaattgc
    21481 tttacgcagg cgcatctggc acacgatgat aaacgcccat gaagcgatga ttcccagcga
    21541 cgcgaagttc aacacaatct caaatacgcg cgacggcacc agatagttga ggaatacgcc
    21601 gacgacatac acaactagtg tcgccagaat cccggcatac ggcacatgct gacgactcat
    21661 tttcgccata aaactcggtg cggaaccgcc catcgccatt gagcgcagaa tacgtccggt
    21721 gcagtacaga cctgagttca ggctggagag ggcagcggtc agcaccacaa tgttcataat
    21781 gctgccgata tatggcacac ccagtttaga gaaaaacgtc acgaacggac tttgccccgc
    21841 ctgatacgcg ctccacggca ataacataac cagcaacacc acggagccga cgtaaaacag
    21901 gccaatacgc caaatcacac tgttaatggc tttaggcacc atggtctgcg gatctttaca
    21961 ttctccggca gctgtaccca ccatttcaat ggaggcaaaa gcaaacacta cgccctgaat
    22021 caacaccagc gcaggcagca aaccgtgggg gaagaagccg ccattatcgg tgattaaatg
    22081 aaagccagtg gtgttgccat ccagcggctg accactaccg aggaacactg tacccacgac
    22141 caaaaaggtc acaatggcga gcactttaat aagcgcaaac cagaactcca tctccgcaaa
    22201 ccatttcaca ccgatcatat tcatggtgcc aacgatggta agtgcagcga gcgcaaagac
    22261 ccactgcggc acgccgccaa acgcacccca gtaatgcata tacagagcga cggcggtaat
    22321 atcaacaatc cccgtcatcg cccagttgat gaagtacatc cagccagcaa cataagcggc
    22381 tttctcaccc aaaaactcac gggcataaga gacaaaactg ccactggaag ggcggtgtag
    22441 caccagctca cccaatgcac gcagaataaa aaacgaaaac aagccacaaa ttaaataaac
    22501 cagtgccaat gctggacccg ccatttgcag tcgggctcct gcacctaaaa acaagccggt
    22561 gccaatcgcg ccgccaatgg cgatcatctg tacctggcga ttgcccatcg ctttgtgata
    22621 cccctcttcg tgggcattaa gccagcggcg tttcgcggcg tgttgatctg aagtgtcggt
    22681 gtcgtgttta ctcattgctc tccctgattg ctttaatgaa aaagtcatat aagttgccat
    22741 gaacaatgtt tattcatcct ggcgatgata gttatcacca ttattcttta tgggtataaa
    22801 actatcggcg cagcatccta cccgttatcg ataaacgatg caaaacatcc cctaacaatc
    22861 ctgaagggga ttaatacaac tgacgaaaaa atgacaaatc cttttgctgg ttaacctgtg
    22921 tactgtccta cacttaatct ttaaaagatt gtgaggggca tatgattaag gtgtatggcg
    22981 tacccggatg gggctctaca atcagtgagc tgatgctaac actggctgat attccttatc
    23041 agttcgttga tgtcagtggt tttgaccacg agggagcttc acgcgagtta ttgaaaaccc
    23101 tgaacccgtt gtgtcaggtt ccgacactgg cgctggaaaa tgacgagatt atgacggaga
    23161 cagcggcgat tgcattgatg gttctcgatc gtcgcccgga ccttgcgcca ccggttgggc
    23221 gcgccgaacg tcagcagttt caacggctat tggtctggct ggttgctaat gtctatccaa
    23281 cattcacttt cgccgattac cctgagcgtt gggcatctga cgcacctgaa cagttaaaga
    23341 agaatgtaat tgaatatcgc aaatcacttt atatctggct gaattcacaa ttgaccgctg
    23401 aaccttatgc ctttggtgag caactaacgc tggtggactg ttatctctgc accatgcgca
    23461 catgggggcc ggggcatgaa tggtttcagg ataatgcgca gaatatcagc gcgatcgctg
    23521 atgctgtatg tcagttaccg aaattacaag aagtattaaa aaggaatgaa attatttgag
    23581 tgggatacca gaagattatg ttatttatta ttcagtaact ttatccacaa tgatgggtgt
    23641 aattaattaa attcatgtta tgttttttga tttatatcac tctccccttc attcagcttg
    23701 tctatttcat tttctctgaa aagaaactaa ctattcacat tgtgcagatg tttcacttgt
    23761 tatcacaggt attctataat ctcaaaaagt ttttaatgat ggatatgctc ggagttggag
    23821 atgcaattaa tattaataca aataaaaata tccggcaggt atgctaaaag gataaatggc
    23881 gttctctgta tttattcgca attatctgta ggacaaatat ggagagcttg atgagtcaga
    23941 gctaaaacag caattgatta ataaacatgt tgatttattc aagaaaatgg atgtatttta
    24001 aatgttgatt cttaatataa cctaatcaat agattatcaa gtgagagcta cacattattg
    24061 gaggggcagg ggaaaacacc attttgggaa caatgcatca ggtactctta ccactgaagc
    24121 aagatcatcc gggcacctgg tggtggtgca gtgctttgga aatggtcccc tcgggggaat
    24181 tgtcattatt ttctatgcta tttgcttatg atattcgtca ggtgaattta ataaattcct
    24241 aatttgaaat atccactatt aagccagtgt ttaacgacgt taagatggca atgtgcagat
    24301 catgttatca gggtggggcg gtagcgtata ttccttatac aaatcatcat tcatttcaat
    24361 gattgttatt gaattattta ttgttgtgaa taaaaagaca ttatgaatat tggtttgact
    24421 ttgcatatca ggttttttat tgctcgctgt aatgtacgag cctgtcgtga ataacgtcga
    24481 gaacccatcc gttgcgtttg tgattatttt gttgactaaa cagacacccg tttctctgaa
    24541 gtaaaatccc agactaaatc atcacataac catgacattt ttcttatatt ccccggtaac
    24601 gccagatgtc gactcgctta tctcatccgc ccgacatgtt gtacatgcaa tatgtgcgga
    24661 ttattattca ttttcacgga ggttgctatg tcaaccggat tacgtttcac actggaagtg
    24721 gacggcctgc cgccggatgc ttttgcggtg gtttcctttc atctgaacca gttactctct
    24781 tcgctctttt ccctcgatct ctccctggtc agccagcagt ttctctccct tgaatttgcg
    24841 caggtgctgg acaaaatggc ctacctgacg atatggcagg gcgatgaagt acagcgccgg
    24901 gtgaaaggcg tggtgacctg gtttgaactc ggggagaacg acaaaaacca gatgctgtac
    24961 agcatgaagg tgcacccgcc gctgtggcgt gccggtctgc gccagaactt ccgtatcttc
    25021 cagaacgagg acatcaaaag catcctcggc acgatgttgc aggaaaacgg ggtgaccgaa
    25081 tggagtccgc tgttcagcga gccgcatcct tcccgtgagt tttgtgtcca gtacggtgag
    25141 actgattacg atttcctgtg ccggatggcg gcggaggaag gcatcttctt ttatgaggag
    25201 catgcttaca aaagtaccga ccagagcctg gtgctgtgcg acacagtccg ccatctgccc
    25261 gaatcttttg aaatcccatg gaacccgaac acccgtaccg aggtgagcac cctctgcatc
    25321 agccagttcc gctacagcgc acaaatccgc ccttcttccg tggtgaccaa agactacacc
    25381 tttaaacgcc ccggctgggc cggacgtttt gaacaggaag gccagcacca ggactaccag
    25441 cgcacgcagt atgaagtgta tgactacccc ggacgtttca agagcgccca cgggcagaac
    25501 tttgcccgct ggcagatgga cggctggcga aacaacgcag aaaccgcgcg gggaatgagc
    25561 cgctcgccgg agatatggcc gggacggcga attgtgctga cggggcatcc gcaggcgaac
    25621 ctgaaccggg aatggcaggt ggtggcaagt gaactgcacg gcgaacagcc acaggcggtg
    25681 ccgggacgca gtggttcagg caccacgctg aataatcact ttgcggtgat cccggcagac
    25741 agaacatggc gaccacagcc gttgctgaaa ccgctggtgg acggcccgca gagcgctgtc
    25801 gtgacaggac cggcaggcga ggaaatcttc tgcgacgaac atggtcgcgt gcgggtgaag
    25861 ttcaactggg accgttataa cccggcagac caagacagtt cgtgctggat ccgtgtggca
    25921 caggcgtggg caggcaccgg tt
//
LOCUS       DAEVZA010000067        25667 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEVZA010000067 DAEVZA010000000
VERSION     DAEVZA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.067"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..205)
                     /locus_tag="LAF92_002738"
     CDS             complement(<1..205)
                     /locus_tag="LAF92_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBI9791723.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLESIIICS"
     gene            complement(183..389)
                     /locus_tag="LAF92_002739"
     CDS             complement(183..389)
                     /locus_tag="LAF92_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HBI9791724.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            complement(386..997)
                     /locus_tag="LAF92_002740"
     CDS             complement(386..997)
                     /locus_tag="LAF92_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HBI9791725.1"
                     /translation="MAKPARRRCKNNECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVDEIESNHNRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(990..1166)
                     /locus_tag="LAF92_002741"
     CDS             complement(990..1166)
                     /locus_tag="LAF92_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HBI9791726.1"
                     /translation="MLSPSQSLKYQKESVERALTCANCGQKLHVLEVHVCSDCCAELM
                     SDPNSSMHEEEDDG"
     gene            complement(1159..1563)
                     /locus_tag="LAF92_002742"
     CDS             complement(1159..1563)
                     /locus_tag="LAF92_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 family protein"
                     /protein_id="HBI9791727.1"
                     /translation="MDYSQLSDFEINKRVCEALDMEEHFFIPDDEADFDSEIPTDERG
                     PIWQTQKRDINGFRASNGNCFNPCNNPEYAWPIITENNISIILDNPSMPCATDNARDL
                     FDDAGPNVGVAYDNPLRAAMIVFLMMQDANNA"
     gene            complement(1566..1742)
                     /locus_tag="LAF92_002743"
     CDS             complement(1566..1742)
                     /locus_tag="LAF92_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBI9791728.1"
                     /translation="MARQRRSITDIICENCKYLPTKRSRNKPKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(1709..1882)
                     /locus_tag="LAF92_002744"
     CDS             complement(1709..1882)
                     /locus_tag="LAF92_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninD"
                     /protein_id="HBI9791729.1"
                     /translation="MMRCDRCGECKEDNRFRPNQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            complement(1879..2319)
                     /locus_tag="LAF92_002745"
     CDS             complement(1879..2319)
                     /locus_tag="LAF92_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBI9791730.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(2594..3970)
                     /locus_tag="LAF92_002746"
     CDS             complement(2594..3970)
                     /locus_tag="LAF92_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9791731.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            complement(3967..4797)
                     /locus_tag="LAF92_002747"
     CDS             complement(3967..4797)
                     /locus_tag="LAF92_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBI9791732.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNAEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            complement(4784..4945)
                     /locus_tag="LAF92_002748"
     CDS             complement(4784..4945)
                     /locus_tag="LAF92_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791733.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(4978..5274)
                     /locus_tag="LAF92_002749"
     CDS             complement(4978..5274)
                     /locus_tag="LAF92_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791734.1"
                     /translation="MAQASYSKPTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFRYALDEITKKKSPVAAEDFKQIDMQF"
     gene            complement(5390..5575)
                     /locus_tag="LAF92_002750"
     CDS             complement(5390..5575)
                     /locus_tag="LAF92_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBI9791735.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            5656..6306
                     /locus_tag="LAF92_002751"
     CDS             5656..6306
                     /locus_tag="LAF92_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBI9791736.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            6621..6926
                     /locus_tag="LAF92_002752"
     CDS             6621..6926
                     /locus_tag="LAF92_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBI9791737.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKNRSIYYKDSNPLGNKIHAVQKIKLYSKLPYGAY"
     gene            6929..7267
                     /locus_tag="LAF92_002753"
     CDS             6929..7267
                     /locus_tag="LAF92_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI9791738.1"
                     /translation="MLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAYAL
                     KNGVTPGFNAIDDGPEYKINEEPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVNHA
                     HQRNPNKKWS"
     gene            7471..7671
                     /locus_tag="LAF92_002754"
     CDS             7471..7671
                     /locus_tag="LAF92_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HBI9791739.1"
                     /translation="MTTTIDKNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVD
                     KWAIRTTAMIARELGKQNNKAA"
     gene            7713..8039
                     /locus_tag="LAF92_002755"
     CDS             7713..8039
                     /locus_tag="LAF92_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791740.1"
                     /translation="MKVLMVYENVPESTEIYIFDANEDEVNDLKLSHGNYTNANCDES
                     IEKALSRVLVRISDPEHCDNDWLSYCGAVKTDAGKWSKSKVDNSTPIIMKDSDIEMVI
                     ITGMIM"
     gene            8236..8370
                     /locus_tag="LAF92_002756"
     CDS             8236..8370
                     /locus_tag="LAF92_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791741.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            8355..8507
                     /gene="kil"
                     /locus_tag="LAF92_002757"
     CDS             8355..8507
                     /gene="kil"
                     /locus_tag="LAF92_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HBI9791742.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKEMKSAINL
                     PWYRRK"
     gene            8583..8753
                     /locus_tag="LAF92_002758"
     CDS             8583..8753
                     /locus_tag="LAF92_002758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791743.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            8762..9469
                     /locus_tag="LAF92_002759"
     CDS             8762..9469
                     /locus_tag="LAF92_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HBI9791744.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIKIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKQGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            9470..9943
                     /gene="ssb"
                     /locus_tag="LAF92_002760"
     CDS             9470..9943
                     /gene="ssb"
                     /locus_tag="LAF92_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI9791745.1"
                     /translation="MASRGVNKVIIIGRLGHDPEIRYSPSGTAFANLTVATSEQWRDK
                     KTGEQKEQTEWHRVVMSGKLAEIASEYLRKGSEVYLEGKLRTRKWQDQSGQDRFTTEV
                     IVGVGGTMQMLGGKQGGNEQSSPQRNNGQQQRQQSQQHGNHSEPPMNFDDSDIPF"
     gene            10441..10989
                     /locus_tag="LAF92_002761"
     CDS             10441..10989
                     /locus_tag="LAF92_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBI9791746.1"
                     /translation="MNHLMVDLETMGNGPYAPVISIGAVFFDPNTGETGEEFSVNISL
                     ESSMRYRARPDASTILWWLEQSEEARKSLTSNTQELSTALSWLSEFIIKNANHKFVQV
                     WGNGASFDCVILRNSYSLTGQPVPWQWWNDRDVRTIVELGKVIGFDPKRDMPFKGTRH
                     NALDDAIHQAKYVSAIWKKLAK"
     gene            11006..11302
                     /locus_tag="LAF92_002762"
     CDS             11006..11302
                     /locus_tag="LAF92_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HBI9791747.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYSHFPEYIDPRLRNYRSRYGAISND"
     gene            11313..11477
                     /locus_tag="LAF92_002763"
     CDS             11313..11477
                     /locus_tag="LAF92_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HBI9791748.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPIPSREELLKRNSFPSVNQNKY
                     LNAMLRSGKK"
     gene            11498..12109
                     /locus_tag="LAF92_002764"
     CDS             11498..12109
                     /locus_tag="LAF92_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791749.1"
                     /translation="MDGFLKGKCIPRDLKVNETNAEYLVRKFGELESKLETALRECRS
                     AGITIDNLEAKCVALAAESAVMKAGHSYFSYGSEHNFEWHKTAEEAIESAEAAIDDYR
                     GDACDGWSEEVDSICWGIIMQSSTKVGERPRNEDDCCDPAIDTICDYALLPNIETPAT
                     DAFLAEVRAQGVEMMREHPSIKLCSLTHICDELAAQLRKGGNQ"
     gene            12106..12591
                     /locus_tag="LAF92_002765"
     CDS             12106..12591
                     /locus_tag="LAF92_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI9791750.1"
                     /translation="MTGHAAILDMCCGSRMFWFDKKDDRAIFGDIRKEEHTLCDGRRL
                     IISPDLIADFRALPFADASFPVVVFDPPHLERVGDNAWMGKKYGRLNKDTWRDDLRQG
                     FKEAFRVLRPYGVLIFKWNETQIPVRQILALTDRKPVIGQRTGKGDKTHWIIFMKEGN
                     Q"
     gene            12588..13187
                     /locus_tag="LAF92_002766"
     CDS             12588..13187
                     /locus_tag="LAF92_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HBI9791751.1"
                     /translation="MSKIDYQALRERYSPKPVPECSVCGEEMSIQRISGAHVVYACSS
                     YGGDGDFKIGRTLADEHYEKSRVTVVDDSDPDVIALLDELEAAKSKLNEQREYYEGVI
                     SDGGKRIAELEAREIKPAKGEVLVVVSGFTGCGKSAIAGEIEIAMKAIGVPVQWTNGD
                     AEKRMTGADWLTAIEMYKPTVRIVEVNVPRVAGIRIKGE"
     gene            13189..13821
                     /locus_tag="LAF92_002767"
     CDS             13189..13821
                     /locus_tag="LAF92_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBI9791752.1"
                     /translation="MSTITKERIELFIKSPLENGLTRGEQMELARIALASLEAEPVGA
                     FHIAEQQVDGTSDYIKDGEWPIDNGTIEVYAAPPVPVVPAALPENDDEDGQDIDYLEP
                     SEVYSLGRTAGWNACRAAMLYGAVPVSQTYKLNEMSGNSPVIPDGWISCSERMPNEED
                     VLVYCSDTKEQMVGFHKGKGLFQFFYMNGVEGVCEPSHWMPLPEPPQEAK"
     gene            13818..14078
                     /locus_tag="LAF92_002768"
     CDS             13818..14078
                     /locus_tag="LAF92_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eaa protein"
                     /protein_id="HBI9791753.1"
                     /translation="MMDVKEKVLQVMRSRAALQDKALGGEYPFRMATWNLRLAMEKEF
                     PDEEWRSADLRKILMELAKDGAVSKDTYASRIGQAVWRLEVR"
     gene            14185..14484
                     /locus_tag="LAF92_002769"
     CDS             14185..14484
                     /locus_tag="LAF92_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791754.1"
                     /translation="MACSTFNPITLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            14593..14793
                     /gene="torI"
                     /locus_tag="LAF92_002770"
     CDS             14593..14793
                     /gene="torI"
                     /locus_tag="LAF92_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HBI9791755.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(15323..16570)
                     /locus_tag="LAF92_002771"
     CDS             complement(15323..16570)
                     /locus_tag="LAF92_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HBI9791756.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(16642..17556)
                     /locus_tag="LAF92_002772"
     CDS             complement(16642..17556)
                     /locus_tag="LAF92_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBI9791757.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            17772..19205
                     /gene="cscA"
                     /locus_tag="LAF92_002773"
     CDS             17772..19205
                     /gene="cscA"
                     /locus_tag="LAF92_002773"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBI9791758.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(19213..20271)
                     /locus_tag="LAF92_002774"
     CDS             complement(19213..20271)
                     /locus_tag="LAF92_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI9791759.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            20439..20657
                     /locus_tag="LAF92_002775"
                     /pseudo
     CDS             20439..20657
                     /locus_tag="LAF92_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            20675..22003
                     /gene="dsdA"
                     /locus_tag="LAF92_002776"
     CDS             20675..22003
                     /gene="dsdA"
                     /locus_tag="LAF92_002776"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBI9791760.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(22111..23649)
                     /gene="emrY"
                     /locus_tag="LAF92_002777"
     CDS             complement(22111..23649)
                     /gene="emrY"
                     /locus_tag="LAF92_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBI9791761.1"
                     /translation="MAITKSTPAPLTGGTLWCVAIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(23649..24812)
                     /gene="emrK"
                     /locus_tag="LAF92_002778"
     CDS             complement(23649..24812)
                     /gene="emrK"
                     /locus_tag="LAF92_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBI9791762.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            25228..>25667
                     /gene="evgA"
                     /locus_tag="LAF92_002779"
     CDS             25228..>25667
                     /gene="evgA"
                     /locus_tag="LAF92_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBI9791763.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSK"
BASE COUNT         7128 a         5912 c         6007 g         6620 t
ORIGIN      
        1 atgagcaaat gatgattgat tccaggcatt caacgttctc tgtaccgcga tcgaccaaat
       61 cgccaaccga gataagcagg tcttttttgg tgtcgaatcc tatcgtctcc agttttttca
      121 tcaggttcgt gtagcatccg tgcagatcgc caactaccca aatatttcgg tatttgctgc
      181 catcaatttt ttcgtaatag cgcatctctt tcactccatc cgcgatgaac catgagaacg
      241 tcgttgacga tggcgtgcat tttcccgtct ttatcatcaa cgtattttct gaccgtaccg
      301 cgactacatt tcagtctgcg cgctacttct gtctggtttc cgtatgcttc aacgagcatg
      361 tctggaatgg tttttactga gaacgtcatg cggcctcact tctgctattt cgcaggtctt
      421 tgagtttctg ttggtactct gccttgatcg ccttgcactc ttcgatagtc cagcgatggc
      481 ggttatggtt tgattcgatt tcatctactg cttcctgccc gatgcggcta atcagttcga
      541 cgcgatacgg aacgagattt ccgcttttgt gctggttgca caccacgcat tgcttgtgaa
      601 tattgcgttc atcaaatcgg agttgaggtg ccgcagcagt tgtccggtaa tgtccggcat
      661 cccactgagc agacgtgagc gttccgcacg agatacatgg taagtcgcgg tctctttctc
      721 tgatgaaggc gtttacggct tgttgggctt gtttaatcca gtaactgcgg ggctttaagg
      781 cgagttttcg aatcttaagt ttatctttct gtttctgctc ctctcgtcgt cgtttcttct
      841 ctgctgcttt ttccgctttt tcgcgttctt tgcttcgtcg ttcgagtgct atctttgttc
      901 cacactctgg agagcaccac cactgattag cgaatgcagg gtgaaaccat tcccgacatt
      961 cattgttttt acatcgtctt cgcgctggtt tagccatcgt cttcttcctc gtgcattgag
     1021 ctattcggat cgctcatcag ttctgcgcag caatcggagc acacgtgaac ttccagcaca
     1081 tgcagcttct gaccgcagtt agcgcacgtt aaagctcgct cgacgctttc tttctggtat
     1141 ttaagggatt gggatgggct aagcattatt ggcgtcctgc atcatgagga agacaatcat
     1201 ggcggcacgg agtggattgt catatgcgac accaacattc ggtccggcat catcaaacaa
     1261 gtcccttgcg ttgtctgtgg cgcacggcat tgagggattg tctaaaatta tgctgatgtt
     1321 gttttcagtg ataatcggcc atgcgtattc aggattattg caaggattga agcaatttcc
     1381 gtttgaagca cggaagccat taatatccct tttttgcgtc tgccaaatag gacctctttc
     1441 gtcagtgggg atctcagaat cgaaatctgc ttcgtcatca ggtatgaaga aatgctcctc
     1501 catatctaaa gcctcacata ctcgcttgtt aatttcaaaa tcacttaact gtgaataatc
     1561 cattgtcatt tcctcgcacg atgccttagc cacctgatat cccacaggtg agccgtgtag
     1621 ttgaaggttt ttacgtcaga ttcttttggg attggcttgg gtttatttct ggagcgtttc
     1681 gttggaaggt atttgcagtt ttcacagatt atgtcggtga tacttcgtcg ctgtctcgcc
     1741 atacgtcctc cttttcctgc ggtagtggta aaactcctgt tggtgttctt tcacaccgga
     1801 gacaccatcg attccagtaa ggttgatttg gtctgaagcg gttatcttct ttgcattcac
     1861 cgcaccgatc acatcgcatc atgctgcccg gtctccccat cgcgctttcc attcgagagc
     1921 cagtcgcgct tcgtctgacc acttaacgcc acgctctgta ccgaatgcct gtataagctc
     1981 taatagctcc gcaaattcgc ttacacgcat cctgctggtt gactggccta ttaccacaaa
     2041 gccattcccg gcaaggttag gaacaacgtc ctgctgcttt aatgctgcgg taaacacaca
     2101 cttccagctt tctgcatcca gccagcgacc atgccattca acctgacgag agacgtcacc
     2161 aaggcaagcc caaagcttcc tgttttggtc taagctgcgg ttgcgttcct gaatggttac
     2221 tacgattggc ttggttgggt ctggaaggat ttgctgtact gcgtgaatag cgttttgctg
     2281 atgtgctgga gatcgaattt caaaggttag ttttttcatg acttccctct ctaacagatt
     2341 tcaggttatt ccactccgtt accgcactgc gataattcgc ggccgccaca gcagcgtggt
     2401 tagcgcagta gatttggcat ccgttctcca tgtcgaatat tgtcggtgat tttccgcatt
     2461 tacatttttt ggcacgcggt gcgtctgaac atattccgtt aacggtgtcc atcagaatcc
     2521 ccctcgttct taatccaata aaaaagggct actgtgtaaa tagcccctgt tattagctca
     2581 gtgatgtaga tggtcatacg tcagcccctt gtgcatatcg tctgccacgc gcagcaggtg
     2641 catttgatgc tgtgcaaatc tgtctggctt catcctggtc gcatgcaaca aagtgtccgt
     2701 tgcagaaccg ctggtaaacc gtaccaagtg agccaaaacg gtttttcgtc acgatgattt
     2761 cagcaaatgg cgcggcgcta ctgttctcgt catataccgc ttcccgatag agcatgatga
     2821 ttgagtctgc gtcctgctca atgcttcctg aatcacgcaa atctgcgttt gtcgggcgtt
     2881 tgtttggtcg cttctcaaca tcgcgcgaaa gctgacttag ggagataaca ggcgttttca
     2941 agtctttcgc catcgccttc aggcttcctg agatgtgagc aattgcgagg tcgttgcggt
     3001 ctgctttcgg cttctcaatc aggccaagat aatccgccat gatgagtgag aggtttggat
     3061 tttcctgttt gtgccgttct gcgattgagc gtatttcttc gaccgataac cgcgaggcat
     3121 cgactaccca tacatccaaa tctgcaagct gactcatgcc gttagcaaca cgtgcccagc
     3181 cctcgtcatc cattgatgca ggatttcgca gtacgctaac cgacatcctc ccggcgttgg
     3241 caatgcttcg ctctgcaatc tgcaatgcgc tcatttccat tgagaaaatc aatactccgc
     3301 gccggacgtc agaaccagga ataacgcggc ttgcaacgcc ttcggcaatc ttcagcgcca
     3361 gttcggtttt ccccatacca ggacgagcag cgattatcac caggtcttcc gcgttcatcc
     3421 ctccggtgat ggcatcaagt tcttcgattc cggtcttcag ggtatctgac tcttctccgt
     3481 tcctcagacg cctgtcaagc gtgtcagtgt agtcagtaat gatttcccct aaccgtacag
     3541 gtttaacctc gtcacggggc tttctgatgg ctgaaagacg ctttacaagt tcatccatcg
     3601 cctgactcga tgcgtcgatg gttccgctct gaattggttc acgcatttca tccatgattt
     3661 ccagcaccag acggcggtga tagttatccg cgaccattcc ggcatatccc ttcaggtttg
     3721 cggcactcgg gcagtttttg ctggtcatca ggattgacgt gaaatgctcc tctccgcacg
     3781 cctcggcaac catcagcgcg tcgattaggt ttctgtttcg cgcctgcttg cggataacct
     3841 cgaaggcttt ccggtagagc ggaattgaaa acgcttccgg ctccagcgtt gcaagaacgt
     3901 cactggcggt tggagttaat ccaccaatca gcaggccacc gataacgctc gcttcgatat
     3961 cctgtctcat gcaatccccc tgtctgcaaa cttcccttcc cgtactcccg ttaacgaatc
     4021 ttccctcagc aggtaatcaa aatcagccgt ccagcccgtg tcgttgtctc cgaagtaaaa
     4081 cggtttggcc tgatgcacaa acgccctgac atacgctctg aaaccgtcca catttggcgt
     4141 tttcagttgc gggatgattt tcttcaggcg gcgtttgcgt ttctcgttga ccgcaacagc
     4201 gtgtggcagt ctgtcaccga cttcggcgtt gtaggcgttc aggaaggatt cgtagtcgat
     4261 tcgttctgcc ttgcgacgtt caggtttaac ctgcccatcg ccgcccccgt tagggggtaa
     4321 gggggtattt gtatttattg tcttttgtat attgtctttt gtgtttgact gattcggtaa
     4381 attgcttttt accgatttgg tgaaggtttg ttttaccgaa ttagtaaatg ttttaccgaa
     4441 tccgttaact ttcgttttcc actcggaaat gtttgtattc ataccaactt gacgccccac
     4501 ctgaatgaga actcccattc tgataagctc gtttttggcg gtagagcatt tggttggtgc
     4561 catgccagtg agttcagcga actgttcgtt tccgatccaa tctatttttt tgttgtaacc
     4621 gtatgtcttg cgccacacag ccataacaat cagtaactga tgtagagtaa gcccagaaag
     4681 catggcggct tctaacagtg tgtttgcagt ccgagtgtag ccatcttcga gttctgccac
     4741 gcgatgctcc acgacctcca gttgaggcct gtaatcagct aacttaacga cgcccatgtt
     4801 tcactcctgc tttggctagt ctgtaaacac caacaaggcg ctctgcgaac gccctgttat
     4861 ttgctgcggc taccactaat ccctcaggtg aatcagggtg tcgaatctct tctttttcct
     4921 ggtatttctt acgacgtttt gtcataatta ctcctgtgga ttgatccagt aattccctca
     4981 gaattgcata tcaatttgct taaaatcctc ggcggccacc ggggattttt tctttgtgat
     5041 ttcatcaagc gcatacctaa aagccctgct aatcggactg atgtctgatg ccattccgaa
     5101 agcacacaag accgaagcaa taaatctcca gtccgttctg cttatcttcg attcatgaca
     5161 gccaatcatc tttgccagac cgcgctgggt aagcgttgac aggttgatga gtaaatcagt
     5221 ttcagcgcga tcaatttctc gctgtgttgg cttgctgtag cttgcttgtg ccatttgtta
     5281 attttcctat attgatattg agttatagcg gcacacccaa tggatttgcc gctgatgttt
     5341 gctcacccgg ttagaggtga aagaccagaa ctgttaaaga gcaatttgct tatgccgctt
     5401 ggcggtaagc actttcttga tacttcaggg cgccagctgt aacgatttcc aatcgatagg
     5461 cgtctttctc tgggataact tctttccact gagagactgc tgcatcgcta atgcctagtg
     5521 ctttagcaac agcacgctgg gttccgaagt ggtcaataac atcttttttg tacatagact
     5581 cgctccgaaa ttaaagaaca cttaaattat ccaccaaagg aatcttaagt caagtttatt
     5641 taagatgtct taactatgaa tacacaactg atgggtgagc gtattcgcgc tcgcagaaaa
     5701 gaactcaaga ttaggcaggc tgcccttggc aagatggttg gcgtgtctaa tgttgctatt
     5761 tcccaatggg agcgatctga aactgagccc aatggcgaaa acctattggc cttagccaag
     5821 gctttgcagt gctcccctga ttacctgttg aaaggagagg atagtctttc aaacattgcc
     5881 tatcacagca ggcatgatcc aagaggttcg tatcctctaa ttagttgggt aagcgcagga
     5941 tgttggatgg aagctgtaga gccatatcat aggcgtgcaa tagataactg gtacgacaca
     6001 acggtagatt gttctgaaga ctctttttgg ctcgacgtta aaggcgattc aatgactgcc
     6061 ccggcaggac tgagtattcc tgaggggatg attattctcg tcgacccaga agtcgaacca
     6121 cgtaatggaa agctggtagt cgccaaactt gaaggagaaa acgaggcgac attcaaaaag
     6181 ttagttattg atgccggtag aaaattcctg aaaccactca atccacaata cccaatgatt
     6241 gaaatcaatg ggaactgtaa aatcattggc gttgtcgttg atgccaagct agcaaacctt
     6301 ccttaagggg ctttcgcccc ttttttattt cccgttaaaa atcaaagaca aactaaattc
     6361 acgtccataa aattaagttt tcttcaaaaa tgcacttgac caataaatta agaagtctta
     6421 aatttaagcc atcagcagga cgctggaagc caaacggaac agattggcag gctctttaac
     6481 atcgacggac tctcaaccta accgttgaga ccagaacttg agtggttttg gggatggcgc
     6541 gaattgcagc tgcaagacag cgatcgagaa gataagcacc tcgacgcgtc atgcgccaaa
     6601 gccacttaaa ggagaccatc atggtaacca ttgtctggaa agaatccaaa ggtacggcaa
     6661 aaagccgcta caaagctcgc agagcagaac ttattgccga gcgacgcagt aatgaagcac
     6721 tggcgcgaaa aattgcgcta aagctctctg gttgcgtcag agcagacaaa gcagcatcac
     6781 taggaagcct ttgctgcaag aagaaagaag aagtcgttcg aaaaaataga agtatttatt
     6841 acaaagattc aaacccatta ggaaacaaaa tacatgcagt ccaaaaaata aaattgtaca
     6901 gtaaactacc gtacggtgct tattgagtat gcttatggtg aaaaagacta tttatgttaa
     6961 tcctgaccgc ggacaaaaca gaaaagtatc tgatagaggt cttacatctc gagacaggag
     7021 gagaatagcg agatgggaaa aaaggatagc atatgcatta aaaaacggtg taacacctgg
     7081 atttaatgct atagatgacg gtcctgaata taagattaat gaagaaccaa tggacaaagt
     7141 tgacaaagca ttagcaacac catttcctcg cgatgtcgaa aaaattgaag atgaaaaata
     7201 tgaggatgta atgcacagag ttgttaatca cgctcaccaa cgaaatccaa ataaaaaatg
     7261 gtcatagccc acttcggtgg gttttttatt gtctgaacaa acctaattta ctaccgcaag
     7321 ccacgcagtg aaatgggtgt gacttgtgtt ggtcgccaga aaatgaaatt aggcagcaaa
     7381 ccacttattt gaggtgagat atggaagaag aatttgaaga gttcgaagag catccgcagg
     7441 atgtgatgga acaataccag gactatcctt atgactacga ctattgataa aaatcaatgg
     7501 tgtggacaat tcaagcgatg caatggatgc aagctgcaat cggaatgcat ggttaagcct
     7561 gaagaaatgt ttcctgtaat ggaagatggg aaatatgtcg ataaatgggc aatacgaacg
     7621 acggcaatga ttgccagaga acttggtaaa cagaataaca aggctgcctg atggtggcct
     7681 ttatttttgg cataaacaat attggggtaa aaatgaaagt tttaatggtt tatgaaaatg
     7741 ttccagagtc gactgaaatc tatatttttg atgccaatga agatgaagtt aacgatttaa
     7801 aattgtctca cggcaattac acaaatgcta attgtgatga aagtatcgaa aaagcactat
     7861 cacgtgttct tgtcagaatc agcgatccag aacattgtga taatgattgg ctttcttatt
     7921 gtggagcggt aaaaactgat gcaggaaaat ggagtaaaag taaagttgat aactcaactc
     7981 caatcattat gaaagatagt gatattgaaa tggtaataat aaccggaatg attatgtagg
     8041 atgcgaataa gcactgtgta ttcattccaa cgagtgaata cacggagcaa tgtcgctcgt
     8101 aactaaacag gagccgactt gttctgatta ttggaaatct tctttgccct ccagtgtgag
     8161 ggccttttta tatgcatacc aataatgctt cacgagaggc attttcgtta tgcaatcaaa
     8221 tataaggagt tacccatgat gcactttcag ctcgcgggta gcggcgtcat gtccgctttc
     8281 tacccgcacg aatctgaatt atcacgccga gttaaacaat taatcagagc agcaaagaaa
     8341 caactggagg cgttatgcgc aatgaaatag ccatcaatca ccagatgctt cgtgctgcac
     8401 agaacaaagc agtaatagcc agatttattg gtgattccaa aatgtggctt gaagcaaata
     8461 aagagatgaa atcagctatc aaccttccgt ggtatcgcag gaaatgagtt ttacagataa
     8521 ctggtcagac gaagaattca ttcgtcagat gaaagaatta atcggtaacg aaggagatat
     8581 tcatgtcact tgcaaccaca gtgaaggaga gcaagttaca gagacgcatg tacacgcagc
     8641 aggcgttaat gtatcgccag aagggagatc gtgaaggtgt tcgcgtattt ttaaatgcgg
     8701 caaagactga agtattaaat cagcgttatt tccttgggcc atgtccattc tgaggtgaat
     8761 tatggatttg aataaattcg atgagccatt cagccccgaa gatatcgaat ggcgaataca
     8821 gcaaagcggt aaaacacgcg atggcaaggt gtgggctatg gtgctggctt atgtcacgaa
     8881 ccgggcaatc atgaaacgcc tggacgatgt ttgcggcaaa gcaggatggc gcaatgaata
     8941 ccgcgatatt cccaacaacg gcggagttga atgcggcata tcaatcaaga ttgattccga
     9001 atgggtaacc aaatgggatg ctgctgaaaa cacgcaggta gaagccgtca aaggtggtcg
     9061 ttccggtgca atgaagcgtg ctgccgttca gtggggaatc ggtcggtatc tgtataacct
     9121 tgaggaaggt ttcgcacaaa catctctcga caaaaagcag ggatggcaca gggcaaaact
     9181 caaggatgga acaggatttt actggctccc tccatcgctg ccgggctggg caatcccagc
     9241 atcagataac aaaccatcac cagaaaatac caaccagaaa tctccatcgg ttgactgcga
     9301 acaaatcctg aaagacttca gcgattatgc gtcaacagaa actgacaaga aaaaactcat
     9361 cgagcgttat cagcgtgact ggcaattaat ggctggcaac gaggaggcgc aggctaaatg
     9421 cgttcaagta atgaacatca gagttaacga actaaaacag gcggcataaa tggcaagcag
     9481 aggcgtaaat aaggtgatca ttattggtcg ccttgggcat gatccagaaa tcagatattc
     9541 accatcagga acggcatttg caaaccttac cgttgctacg tcagaacaat ggcgtgataa
     9601 gaaaactgga gagcaaaagg agcagacgga gtggcaccgc gtggtaatga gcgggaaact
     9661 ggcagaaatt gccagcgaat atctgcgaaa aggctctgag gtttatcttg aaggcaaatt
     9721 gcggacaaga aaatggcagg atcaaagcgg acaggatcgg ttcactaccg aagttatcgt
     9781 aggcgttggt ggaaccatgc aaatgcttgg tggcaagcaa ggaggcaatg aacagtcttc
     9841 acctcagcga aataacggcc agcaacaaag acagcaatct cagcagcatg ggaatcacag
     9901 cgaaccacct atgaacttcg acgattcgga tattccgttc taggagctga atatgaaaat
     9961 ctgctcaaga tgccatcaac agaaggaaga aagggacttt caaatcagaa gagcatccag
    10021 agatggatta actgccgctt gccgggcttg cctggctgaa tacgacaaag aacgcgctgg
    10081 attgccacat cgagtatcag caaggagaga atatcaatca tcggaacgcg gaagagaacg
    10141 gtgtaacgca gccaaaaagc ggttcattca gagcaaccca tggaaaagaa aggcccacat
    10201 cattgtgggt aattttttgc gcgacggtaa gttaatccga ccaccacaat gtgagtgctg
    10261 cggatcagaa tgtaaaccac aggcgcacca ctgcgactac agcaaaccaa ccgatgtgat
    10321 gtggctctgc aagtcatgtc atgtcgagtg gcacaaacat aacaaaccta tctacccaga
    10381 cgaggaacca gtaactctcc ccttccctcg tcacgctatt cacgcaattt aaggacttac
    10441 atgaatcact tgatggttga ccttgaaaca atgggcaacg ggccatacgc gccagttatt
    10501 tctattgggg cggtattctt tgacccgaat accggagaaa caggagaaga gttctcggta
    10561 aatatctcgc ttgaatcatc aatgcgatat cgagctcgtc ctgacgcttc aacgatttta
    10621 tggtggctgg aacagagtga agaagccaga aaatcgctaa ccagcaacac tcaggagctt
    10681 tcaacggctc tttcatggtt atctgaattc atcataaaga acgctaacca caaattcgtt
    10741 caggtttggg ggaatggagc atcatttgac tgcgttattc tccgaaacag ttattcgctg
    10801 acagggcagc cagttccatg gcagtggtgg aatgaccgcg acgtaagaac aatcgtcgag
    10861 cttggaaagg taataggatt cgaccctaag cgagatatgc cattcaaagg aactcgccac
    10921 aacgctcttg atgatgccat tcaccaagcc aaatacgttt cagcgatctg gaaaaagtta
    10981 gctaaataat caacaggaga aaaccatgcc agcgcctctg tatggtgcgg atgacccgcg
    11041 ccgatgttcc ggcaattccg tatcggaggt gctggataaa ttcagaaaaa actacgatcg
    11101 gataatgtct ctaccgcagg aaacgaaaga ggaaaaggaa tttcgccact gtatatggct
    11161 tgcagagaaa gaagaacgag agcgaattta ccagacatca atccgaccat tccgcaaagc
    11221 aacatatagc cacttccctg aatatatcga cccgcgcctg cgtaattacc gctcacgcta
    11281 tggcgctatc agtaatgact gaggaattta ccatgagagg acttgcatac aatcccggca
    11341 ttcttccggc agaaatgatt attcgccaac gcgtaaagcc aataccatcg agagaggaat
    11401 tacttaagcg aaactcgttt ccatcagtga atcaaaacaa atatctgaac gcgatgttgc
    11461 ggagtgggaa gaaatgaaac aaatgtcact aattgagatg gatggatttc tgaaaggtaa
    11521 atgcatccca cgagatttaa aggttaacga aacaaacgct gaatatcttg tccgtaagtt
    11581 cggtgaactt gaatcaaaac tagaaacggc gttgcgggag tgtcgttctg ctggaatcac
    11641 gattgataac cttgaggcca agtgcgtggc gctggcagcg gagagtgcgg tgatgaaggc
    11701 gggacattca tatttctcat atggctctga gcataatttc gaatggcaca aaactgctga
    11761 ggaagctatc gaatcggctg aggctgcaat tgacgactat cgcggtgatg cttgcgatgg
    11821 atggagcgaa gaggtcgata gtatttgctg gggaatcatc atgcaatcgt caacgaaggt
    11881 tggcgaacga ccacgcaacg aggatgactg ttgcgatcct gcgatcgata cgatttgcga
    11941 ttatgcgctt ctgcctaata tcgaaacccc agccaccgat gcttttctgg ctgaagtacg
    12001 ggcgcagggg gtagagatga tgcgcgaaca tccatcaatc aaactttgct ctttgacgca
    12061 catatgtgat gagttagccg cccagcttcg caaaggaggc aaccagtgac tggacatgca
    12121 gcaatcctcg acatgtgctg tggcagtcgc atgttctggt tcgataagaa ggacgaccgg
    12181 gcgatatttg gcgatatcag aaaggaagag cacacattgt gtgatgggcg acggctgata
    12241 attagccctg acctgatagc agattttcgt gcattaccat ttgcagacgc atcgtttccg
    12301 gttgttgtat tcgaccctcc gcatcttgag cgtgttggtg ataacgcctg gatgggaaag
    12361 aaatatggac ggctgaataa agatacctgg cgtgatgatt tgcggcaggg atttaaagaa
    12421 gcctttcgtg tgttgcgtcc atacggcgtt ctgattttta aatggaatga aacgcaaata
    12481 cctgttcgcc agatattggc actgaccgac agaaagcctg ttatcggtca acgaacagga
    12541 aagggtgaca agacccactg gattattttt atgaaggagg gcaaccagtg agcaagattg
    12601 attatcaggc gctacgggaa cgttattcac ctaagccagt acctgaatgc tctgtttgtg
    12661 gcgaggaaat gtcaatacag cgtatatctg gagcacatgt cgtttatgcc tgctccagtt
    12721 atggtggtga tggagatttc aaaattggtc gaacacttgc cgacgagcat tatgaaaaat
    12781 cacgcgtaac agtagttgat gacagtgacc ctgatgtgat tgcactactg gacgaactgg
    12841 aggcagcaaa atcaaaactc aacgagcagc gtgaatatta tgaaggtgtt atctcggatg
    12901 ggggtaagcg cattgctgaa ctggaagcac gggaaataaa accagccaaa ggtgaagttc
    12961 ttgtcgttgt ttctggtttt actggttgcg gaaaaagcgc cattgccggg gaaatagaaa
    13021 tcgcgatgaa ggctattggt gtaccggttc agtggactaa tggcgatgca gaaaagcgca
    13081 tgaccggcgc tgactggctg acagcaattg agatgtacaa accaactgtg cgcattgtgg
    13141 aagttaatgt gccacgcgtc gctggcattc gcatcaaagg agagtgatat gagcactatc
    13201 actaaagaac gtatcgaatt gttcattaaa tccccgcttg aaaacgggct tacccgtggc
    13261 gaacaaatgg aactggcacg aattgcactg gcctcgcttg aggcagagcc agttggtgca
    13321 ttccacattg ctgaacagca agttgacggc acaagtgact acatcaagga tggggagtgg
    13381 cctattgata atgggacaat tgaagtctac gccgccccgc cagtaccggt agtacctgct
    13441 gcattacctg agaacgacga tgaggacggg caggacattg attatcttga gccatctgaa
    13501 gtttactcgc ttgggcgaac agctggctgg aacgcctgcc gtgctgccat gctctatggt
    13561 gccgtacctg taagccagac ttacaagttg aatgagatgt cgggcaactc tccggtaatt
    13621 ccagatggtt ggataagctg tagtgagcga atgccaaacg aagaagatgt tttggtttat
    13681 tgttcagaca caaaagagca gatggtaggg tttcacaaag gtaaagggtt atttcaattc
    13741 ttttacatga acggtgttga gggggtatgt gagccgtcac actggatgcc gctaccagaa
    13801 ccgccgcagg aggcgaaatg atggatgtaa aagagaaggt tttgcaggtg atgcgttccc
    13861 gggctgccct gcaagataaa gctctcggcg gggaatatcc attcaggatg gcaacctgga
    13921 atctgcggtt ggcaatggag aaggaatttc ctgatgaaga atggcgttcg gcagatttgc
    13981 gcaaaattct tatggagctg gctaaagacg gagcagtatc caaagatacc tatgccagcc
    14041 ggattggtca ggcggtatgg agactggagg tgcggtaatg gctaacctgc aacttgccgt
    14101 caaaggtgaa taacaatcct cgcactcgcg gggatttctt ttatctgaac tcgctacggc
    14161 gggttttatt ttatggagat gattatggcc tgttcaacat tcaaccctat aacgttacag
    14221 aaataccagc cagaccctga agatttatgc tcactgtgtg gaggaaatca tggtaaagcc
    14281 gcaatgatcg aatgtaagga caaaatccac atatgcctta attgcgttga tgtcctcgtt
    14341 gatatcaaaa atgaaagaga agataaaaag cgtagcgagg ctattcgcgc cttagattca
    14401 tggatgcgag atgggtatag tgctgcgcaa atttatgact tagccatttc aaaaggcgaa
    14461 ataccaggtg tgcgaatcga ataaggcgta accaatattc gaattgaaga actgaaagaa
    14521 caccaagccg cctgatggcg gttttttatt gcctgatttg caggttcgat tccctattcg
    14581 gagatagcac tcatgcaaca cgaactacag cctgattcac tggttgattt gaaattcatc
    14641 atggccgata ctggcttcgg taaaaccttc atctacgacc ggattaagtc cggcgacctg
    14701 ccaaaagcca aagttatcca cggacgagca agatggttat atcgtgacca ttgtgaattc
    14761 aaaaataagc tcttaagccg cgctaatggg taaaatagcg ggtaaaatat ttctcacatc
    14821 taaaaaatat cattccaatc aatcccctgc tgcttcaagt agatgtctgc aggggacacc
    14881 agataccctt caaacgaaat ctaccttcac cccgtaaaag atgggtttgg cagcacactt
    14941 gccttatatc tactcatttt tactgcaaca ggttgaaatc tcagcactgt cagaaagcgc
    15001 tgatgactaa acagccctgg gccgggcgat gtaaccatca cacagaatcc tgatagcgaa
    15061 atatggcgtg actcgatact tcactccgca atgcattcct tgatgaattc gcaggaccgt
    15121 gatacacggg acaggtcact gaatgacgac aatgtcctgg aaatcagcga accgcgcatc
    15181 tgaagtacat ttgagcgact gtaccagaac atgaatgagg cgtttggatt aggcgattat
    15241 tagcagggct aagcatttta ctattattat tttccggttg agggatatag agctatcgac
    15301 aacaaccgga aaaagtttac gtctatattg ctgaaggtac aggcgtttcc ataactattt
    15361 gctcgcgttt tttactcaag aagaaaatgc caaatagcaa catcaggcag acaatacccg
    15421 aaattgcgaa gaaaactgtc tggtagcctg cgtggtcaaa gagtatccca gtcggcgttg
    15481 aaagcagcac aatcccaagc gaactggcaa tttgaaaacc aatcagaaag atcgtcgacg
    15541 acaggcgctt atcaaagttt gccacgctgt atttgaagac ggatatgaca caaagtggaa
    15601 cctcaatggc atgtaacaac ttcactaatg aaataatcca ggggttaacg aacagcgcgc
    15661 aggaaaggat acgcaacgcc ataatcacaa ctccgataag taatgcattt tttggcccta
    15721 cccgattcac aaagaaagga ataatcgcca tgcacagcgc ttcgagtacc acctggaatg
    15781 agttgagata accatacagg cgcgttccta catcgtgtga ttcgaataaa cctgcataaa
    15841 agacaggaaa aagttgttga tcaaaaatgt tatagaaaga ccacgtcccc acaataaata
    15901 tgacgaaaac ccagaagttt cgatccttga aaactgcgat aaaatcctct ttttttaccc
    15961 ctcccgcatc tgccgctacg cactggtgat ccttatcttt aaaacgcatg ttgatcatca
    16021 taaatacagc gccaaatagc gagaccaacc agaagttgat atggggactg atactaaaaa
    16081 atatgccggc aaagaacgcg ccaatagcat agccaaaaga tccccaggcg cgcgctgttc
    16141 catattcgaa atgaaaattt cgcgccattt tttcggtgaa gctatcaagc aaaccgcatc
    16201 ccgccagata ccccaagcca aaaaatagcg cccccagaat tagacctaca gaaaaattgc
    16261 tttgcagtaa cggttcataa acgtaaatca taaacggtcc ggtcaagacc aggatgaaac
    16321 tcatacacca gatgagcggt ttcttcagac cgagtttatc ctgaacgatg ccgtagaaca
    16381 tcataaatag aatgctggta aactggttga ccgaataaag tgtacctaat tccgtccctg
    16441 tcaaccctag atgtcctttc agccaaatag cgtataacga ccaccacagc gaccaggaaa
    16501 taaaaaagag aaatgagtaa ctggatgcaa aacgatagta cgcatttctg aatggaatat
    16561 tcagtgccat aattacctgc ctgtcgttaa aaaattcacg tcctatttag agataagagc
    16621 gacttcgccg tttacttctc actattccag ttcttgtcga catggcagcg ctgtcattgc
    16681 ccctttcgcc gttactgcaa gcgctccgca acgttgagcg agatcgataa ttcgtcgcat
    16741 ttctctctca tctgtagata atcccgtaga ggacagacct gtgagtaacc cggcaacgaa
    16801 cgcatctccc gcccccgtgc tatcgacaca attcacagac attccagcaa aatggtgaac
    16861 ttgtcctcga taacagacca ccacaccttc tgcaccttta gtcaccaaca gcatggcgat
    16921 ctcatactct tttgccaggg cgcatatatc ccgatcgttc tgtgtttttc cactgataag
    16981 tcgccattct tcttccgaga gcttgacgac atccgccagt tgtagcgcct gccgcaaaca
    17041 caagcggagc aaatgctcgt cttgccatag atcttcacga atattaggat cgaagctgac
    17101 aaaacctccg gcatgccgga tcgccgtcat cgcagtaaat gcgctggtac gcgaaggctc
    17161 ggcagacaac gcaattgaac agagatgtaa ccattcgcca tgtcgccagc agggcaagtc
    17221 tgtcgtctct aaaaaaagat cggcactggg gcggaccata aacgtaaatg aacgttcccc
    17281 ttgatcgttc agatcgacaa gcaccgtgga tgtccggtgc cattcatctt gcttcagata
    17341 cgtgatatcg actccctcag ttagcagcgt tctttgcatt aacgcaccaa aaggatcatc
    17401 ccccacccga cctataaacc cacttgttcc gcctaatctg gcgattccca ccgcaacgtt
    17461 agctggcgcg ccgccaggac aaggcagtag gcgcccgtct gattctggca agagatctac
    17521 gaccgcatcc cctaaaaccc atactttggc tgacattttt ttcccttaaa ttcatctgag
    17581 ttacgcatag tgataaacct ctttttcgca aaatcgtcat ggatttacta aaacatgcat
    17641 attcgatcac aaaacgtcat agttaacgtt aacatttgtg atattcatcg catttatgaa
    17701 agtaagggac tttattttta taaaagttaa cgttaacaat tcaccaaatt tgcttaacca
    17761 ggatgattaa aatgacgcaa tctcgattgc atgcggcgca aaacgcccta gcaaaacttc
    17821 atgagcaccg gggtaacact ttctatcccc attttcacct cgcgcctcct gccgggtgga
    17881 tgaacgatcc aaacggcctg atctggttta acgatcgtta tcacgcgttt tatcaacatc
    17941 atccgatgag cgaacactgg gggccaatgc actggggaca tgccaccagc gacgatatga
    18001 tccactggca gcatgagcct attgcgttag cgccaggaga cgataatgac aaagacgggt
    18061 gtttttcagg tagtgctgtc gatgacaatg gtgtcctctc acttatctac accggacacg
    18121 tctggctcga tggtgcaggt aatgacgatg caattcgcga agtacaatgt ctggctacca
    18181 gtcgggatgg tattcatttc gagaaacagg gtgtgatcct cactccacca gaaggaatca
    18241 tgcacttccg cgatcctaaa gtgtggcgtg aagccgacac atggtggatg gtagtcgggg
    18301 cgaaagatcc aggcaacacg gggcagatcc tgctttatcg cggcagttcg ttgcgtgaat
    18361 ggaccttcga tcgcgtactg gcccacgctg atgcgggtga aagctatatg tgggaatgtc
    18421 cggacttttt cagccttggc gatcagcatt atctgatgtt ttccccgcag ggaatgaatg
    18481 ccgagggata cagttaccga aatcgctttc aaagtggcgt aatacccgga atgtggtcgc
    18541 caggacgact ttttgcacaa tccgggcatt ttactgaact tgataacggg catgactttt
    18601 atgcaccaca aagcttttta gcgaaggatg gtcggcgtat tgttatcggc tggatggata
    18661 tgtgggaatc gccaatgccc tcaaaacgtg aaggatgggc aggctgcatg acgctggcgc
    18721 gcgagctatc agagagcaat ggcaaacttc tacaacgccc ggtacacgaa gctgagtcgt
    18781 tacgccagca gcatcaatct gtctctcccc gcacaatcag caataaatat gttttgcagg
    18841 aaaacgcgca agcagttgag attcagttgc agtgggcgct gaagaacagt gatgccgaac
    18901 attacggatt acagctcggc actggaatgc ggctgtatat tgataaccaa tctgagcgac
    18961 ttgttttgtg gcggtattac ccacacgaga atttagacgg ctaccgtagt attcccctcc
    19021 cgcagcgtga cacgctcgcc ctaaggatat ttatcgatac atcatccgtg gaagtattta
    19081 ttaacgacgg ggaagcggtg atgagtagtc gaatctatcc gcagccagaa gaacgggaac
    19141 tgtcgcttta tgcctcccac ggagtggctg tgctgcaaca tggagcactc tggctactgg
    19201 gttaacataa tatcaggtgg aacaacggat caacagcggg caagggatcc gcgtcactct
    19261 tcccccttca cgaccttcaa taatatgcaa tgcagcttcc cgcccgataa tgtcatgtgg
    19321 aagctgaatt gtggtcagcg gcggtaaaaa cagatgcccg acgccaacca gattatcaaa
    19381 gcccattacg gcgacatcct gcgggattcg tacccccttc gccagaagaa cctgataagc
    19441 cacaaaggct gcgcgatcgt taccacatat cagaacatca aaatctggtt tgcccgattt
    19501 gaagtggtca ttgagtaaac ttgcgagatc ggtgtagtga tcatcacctg ttgccatgtg
    19561 aaattgtttc acctcagcca gatctcgtcc agcatcacgc caggcctgct caaatccctg
    19621 ccgacgatac cctgttgcca acgcactttc cggtagccag aagcataacg gttgacgata
    19681 gcccgccgcg agcaaatgct gtgttgattc atattgtgca gtgtaatcat cagggatata
    19741 actgggtaac gctgggtcat ccgccacaca gttcgccaat acaatatttt caccatacag
    19801 agactcaggc agcgtgatat gtcgcagccc cattgtagta tagataatgc catccggacg
    19861 gtgggcaagc agctgacgtg ccgcgcgggc agcgtcatct tcagaaaaaa tattgattaa
    19921 aaaactattc cagccgaact cgctggcggt ttgctcaatg gcaagcagaa tatcaacaga
    19981 gaaaggagtg gtagccgtgt cctgcgccag cacggcgaga gtcgacggct tacgtccttg
    20041 agcgcgcatc ttacgggcgg aaagatcagg aacataattc agggtctgga ttgcctgcaa
    20101 tacgcggtca cgcgttgcag gacgcacaga ttctgcatta tgcatcaccc gggagactgt
    20161 catcatcgac actcccgcca ggcgtgcgac atcctttaat gaagccatac ccaagccgtt
    20221 tgccgtaaaa cgggcactgt agcagaaaca gacgtcactg gcgagatcca acgccctatc
    20281 acccaacacg gcaatacaat aaaaaataac aataattccc ggacaattgt ccccaattcc
    20341 gcctctgttc tcgccaacga gtaaccatat aggcaagtaa gtaaaacatg ccaactggca
    20401 tcgccaacct ggagaagaaa atataaatat ggatcattgt gctgccgctg tatcccgaca
    20461 tcagcccgga aattattgcg attgctatcg gttcaggtgc aattggctgc acgatcgtta
    20521 cggactcgct tttctggctg gtgaagcaat attgcggcgc tacgctcaat gaaacattta
    20581 aatactatac gacagcgaca tttatcgctt cagtcgtcgc tctggcgggc acattcctgc
    20641 tgtcatttat catctaagcg caaagagacg tactatggaa aacgctaaaa tgaactcgct
    20701 catcgcccag tatccgttgg taaaggatct ggttgctctt aaagaaacca cctggtttaa
    20761 tcctggcacg acctcattgg ctgaaggttt accttatgtt ggcctgaccg aacaggatgt
    20821 tcaggacgcc catgcgcgct tatcccgttt tgcaccctat ctggcaaaag catttcctga
    20881 aactgctgcc actgggggga ttattgaatc agaactggtt gccattccgg ctatgcaaaa
    20941 acggctggaa aaggaatatc aacaaccgat cagcgggcaa ctgttactga aaaaagatag
    21001 ccatttgccc atttccggct ccataaaagc acgcggcggg atttatgaag tcctggcaca
    21061 cgcagaaaaa ctggctctgg aagcggggtt gctgacgctt gaagatgact acagcaaact
    21121 gctttctccg gagtttaaac agttctttag ccaatacagc attgctgtgg gctcaaccgg
    21181 aaatctgggg ttatcaatcg gcattatgag cgcccgcatt ggctttaagg tgacagtgca
    21241 tatgtctgct gatgcccggg catggaaaaa agcgaaactg cgcagccatg gcgttacggt
    21301 cgtggaatat gagcaagatt atggtgttgc cgtcgaggaa ggacgtaaag cagcgcagtc
    21361 tgacccgaac tgtttcttta ttgatgacga aaattcccgc acgttgttcc ttgggtattc
    21421 cgtcgcaggc cagcgtctta aagcgcaatt tgccgagcaa ggtcgtatcg tcgacgctga
    21481 taaccctctg tttgtctatc tgccgtgtgg tgttggcggt ggtcctggtg gcgtcgcatt
    21541 cgggcttaaa ctggcgtttg gcgatcatgt tcactgcttt tttgccgaac caacgcactc
    21601 cccttgtatg ctgttaggcg tccatacagg attacacgat cagatttctg ttcaggatat
    21661 tggtatcgac aaccttaccg cagcggatgg ccttgcagtt ggtcgcgcat caggctttgt
    21721 cgggcgggca atggagcgtc tgctggatgg cttctatacc cttagcgatc aaaccatgta
    21781 tgacatgctt ggctggctgg cgcaggaaga aggtattcgt cttgaacctt cggcactggc
    21841 gggtatggcc ggacctcagc gcgtgtgtgc atcagtaagt taccaacaga tgcacggttt
    21901 cagcgcagaa caactgcgta ataccactca tctggtgtgg gcgacgggag gtggaatggt
    21961 gccggaagaa gagatgaatc aatatctggc aaaaggccgt taataacgtt tcaacgcagc
    22021 atcgcaatcc tttccctggg tgagcgatgc tgccgatggc gcagacttaa gatccccggt
    22081 cttacccgct ataaccccct ccttttatca tcacccaacg cctttcgctg taaacggcgg
    22141 tttcgcaaac caaaccaaaa ccgtaagtaa gataaaagca atagccgcca tacggaaaat
    22201 ctcatttgca gaaatagaaa gtgactgctg ggttatttca ttattaattt cattaagaac
    22261 tcctgaaagc gaaccataat atttatccat aatttgtgac gatgaattaa acacggggtt
    22321 aaattgatcg atggttgctg tcaactgact atggtgtaac gattcgcgtc gtccccacag
    22381 cgtcattgtc aacgacgtac caactgatcc tgacaaggta cgaaaaaaat tactcatact
    22441 cgaggcattg gcaaatttat tatctggcaa gcctgaaaac gaaatcgttg ttaagggtaa
    22501 aaagaaacag gcaacggcga atccctgaaa aaactgcggc aaaatgatgc ctgtaaaatc
    22561 aatcgttggc ataaatgtca cagaacgcca gtaatagcaa accgcataca tcaaaaaact
    22621 aaatgtcact aacaaccgca tgtctatttt gttgccataa cgtcctatca aaggtgaaat
    22681 taatagtggc atgatgccga tgggcgcata agcaagtccg gcccatatcg cattataccc
    22741 catcgtttcc tggagtaact gcggcataag aacgatcgct ccagagtaaa ataaatacgc
    22801 gcatgtgata ctcacaatac caatggtgaa attacgggac ttaaacaaac tgagatcaag
    22861 aatcgggttc tctgaggtcg actcccaaat gactaaagag atcagagaaa taactgatac
    22921 tactgttaat attattattg tactcgagtt gaaccaatcc agatcgcgcc ctttatcaag
    22981 cataatttgc aagccaccaa caccgagcac taacagggtc agtcctggta gattcatttt
    23041 gaccggtgaa gtttcagttt ctcttccttt aagtaaggtt aagcataatg tcaggacgat
    23101 aatccccata gggacattga ttaaaaatat ccaaccccag ctaaagttat cgcaaatata
    23161 accgcccaat atcggcccac atatcggagc gataatcacg gtcattgacc ataatgccag
    23221 agcaaatgtt cttttttctg gtggataatt ccttaataat aaactctgtg acagtggaat
    23281 taacggcccc gccattaatc cctgaacgac tctaaaaaat atcagcacat caagattggt
    23341 cgataaacta cacattaagg aagacagcga aaaaaaagtg actgaaagta aaaataacct
    23401 taattcgcct attctttgtg ccagcctgcc ggtaacaggg atcgcaatgg catttgctac
    23461 gccaaacgag gtgataaccc aggtgccttc gtctgttgat gctcccagaa agccagatat
    23521 tgtcggtatt gcgacgttag aaatagtgga atccaacatt tgcataaacg tcgctaatga
    23581 caatgcaata gcaacgcacc ataacgtccc accggttaat ggtgccggag tcgatttagt
    23641 gattgccatt aaagttgtcc attatgcgaa ataatgttgc taatttcttt ttctatcgga
    23701 ctggtatcga taactaaagc cttactggta taagccggca tggacgtcac ggttgaagcc
    23761 agatcaggca tctcagcaat gtcttcgttc ttcgtatcaa tagttgcagt catcgataaa
    23821 ccaatgcgta aggggtgttc catgagttct tttggatcaa gagaaacttc aaccggtaca
    23881 cgctgaacga ttttgatcca gttccctgtc gcattttgtg caggtaataa ggagaacgca
    23941 ttgccggttc ccatattgat ccctgtcacc cgaccatgaa acacaacatt ttcaccataa
    24001 agatcgctga taatattgac tgattgacca atccgtacat ccgtgagttg tgtttcttta
    24061 aagttggcat taacccacat ttgacgtgcc ggtacgacag ccattaacga ttgtccggga
    24121 ctcactgttt cgccgacctg aacactccgc tgggcaatat agccagtaac cggactcctt
    24181 atatccgtac gtttaagcgc caaccaggct tctttagttg catccgccgc ttccacgact
    24241 tgtggctgac ggtttaatgg tgtgttcatt actaaagctt tattcgcttt ataagcctgg
    24301 atagcggcat tcagtgccgc tttgctactt attaacgtat ctttggtatg ctccagcgtt
    24361 tcttttgaaa taaccccctg cttcgctaac ggtactcgac ggttataatc ttctaaagat
    24421 tgttgatact gaatacgtgc tgaagcgact tcggcactgt attgtttatc ctgtaagtac
    24481 agtttattcg tttgccgaac aatatttgcc agattatttt tagctttatt gagtgcgata
    24541 gtggcatcag ttttatccag tgaaactaaa atgtcacctt gtcgaacgta gttcgtatct
    24601 ttatgattaa cgacagtgac actacctgag acttgtgcag aaattggatc tgcattcccc
    24661 gtgacatagg cgtcatctgt actaatcatg tcttctaatt ccattgacca ataggcatag
    24721 gcacctgaaa acgcaataaa taaaactacc gccaataaag aaaagtattt tcttctgtta
    24781 gaatgttttt tatttgaatt aatctgttcc actattatct ctcatttctc atagatgaaa
    24841 tttatgaatt gaatgaaaag agcattcatt cttcatatca aacacagtgc atgtgttgca
    24901 cattatccca ggaacgttgg aggcctaatt taaatgaggt gcaaaaaaaa caacagagga
    24961 ctaaaccgtg gcttttgcaa tacaattctt acgcctgtag gattagtaag aagacttata
    25021 gtgccaactt gaaactataa atcatcggta caatccctga ttttattgtt gacatttcat
    25081 ttatgccgac tatttatatg gtatacttgt cgaattatct taaaggaagc tcagattttc
    25141 ttatttttat tgagaaaatg agatgacgcc ttatgtctgt attactacag ggagaaggga
    25201 gatgcttcat tgcaaaggga ataatctatg aacgcaataa ttattgatga ccatcctctt
    25261 gctatcgcag caattcgtaa tttattgatc aaaaacgata ttgaaatctt agcggagttg
    25321 actgaagggg gaagcgccgt tcagcgggtg gaaacactta agcctgatat cgtcatcatt
    25381 gatgtcgata tccccggagt taacggtatc caggtgttag aaacgctgag gaagcgccaa
    25441 tatagcggaa ttattattat tgtctccgct aaaaatgacc atttttacgg gaaacattgt
    25501 gctgatgctg gcgctaatgg attcgtgagt aaaaaagaag gcatgaacaa tatcattgcg
    25561 gctattgaag ctgcaaaaaa tggctactgc tatttcccct tctctctcaa ccggtttgtt
    25621 ggaagtttaa cgtccgacca gcaaaaactc gactccttat cgaaaca
//
LOCUS       DAEVZA010000068        25199 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEVZA010000068 DAEVZA010000000
VERSION     DAEVZA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.068"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(163..405)
                     /locus_tag="LAF92_002780"
     CDS             complement(163..405)
                     /locus_tag="LAF92_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791764.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(760..2409)
                     /gene="pgi"
                     /locus_tag="LAF92_002781"
     CDS             complement(760..2409)
                     /gene="pgi"
                     /locus_tag="LAF92_002781"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBI9791765.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
     gene            2934..4283
                     /gene="lysC"
                     /locus_tag="LAF92_002782"
     CDS             2934..4283
                     /gene="lysC"
                     /locus_tag="LAF92_002782"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBI9791766.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4428..5354
                     /locus_tag="LAF92_002783"
     CDS             4428..5354
                     /locus_tag="LAF92_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBI9791767.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(5390..6181)
                     /locus_tag="LAF92_002784"
     CDS             complement(5390..6181)
                     /locus_tag="LAF92_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBI9791768.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVIYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAEQARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(6187..7314)
                     /locus_tag="LAF92_002785"
     CDS             complement(6187..7314)
                     /locus_tag="LAF92_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HBI9791769.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            7844..8116
                     /locus_tag="LAF92_002786"
     CDS             7844..8116
                     /locus_tag="LAF92_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBI9791770.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(8117..8989)
                     /gene="rluF"
                     /locus_tag="LAF92_002787"
     CDS             complement(8117..8989)
                     /gene="rluF"
                     /locus_tag="LAF92_002787"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBI9791771.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            9201..9890
                     /gene="pepE"
                     /locus_tag="LAF92_002788"
     CDS             9201..9890
                     /gene="pepE"
                     /locus_tag="LAF92_002788"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBI9791772.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(9981..11612)
                     /locus_tag="LAF92_002789"
     CDS             complement(9981..11612)
                     /locus_tag="LAF92_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBI9791773.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(11832..15515)
                     /gene="metH"
                     /locus_tag="LAF92_002790"
     CDS             complement(11832..15515)
                     /gene="metH"
                     /locus_tag="LAF92_002790"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBI9791774.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            15715..16539
                     /gene="iclR"
                     /locus_tag="LAF92_002791"
     CDS             15715..16539
                     /gene="iclR"
                     /locus_tag="LAF92_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBI9791775.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            16857..18430
                     /locus_tag="LAF92_002792"
                     /pseudo
     CDS             16857..18430
                     /locus_tag="LAF92_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(18399..20135)
                     /gene="aceK"
                     /locus_tag="LAF92_002793"
     CDS             complement(18399..20135)
                     /gene="aceK"
                     /locus_tag="LAF92_002793"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HBI9791776.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(20416..21720)
                     /gene="aceA"
                     /locus_tag="LAF92_002794"
     CDS             complement(20416..21720)
                     /gene="aceA"
                     /locus_tag="LAF92_002794"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBI9791777.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(21750..23351)
                     /gene="aceB"
                     /locus_tag="LAF92_002795"
     CDS             complement(21750..23351)
                     /gene="aceB"
                     /locus_tag="LAF92_002795"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBI9791778.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(23620..24549)
                     /gene="metA"
                     /locus_tag="LAF92_002796"
     CDS             complement(23620..24549)
                     /gene="metA"
                     /locus_tag="LAF92_002796"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBI9791779.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            24706..25149
                     /locus_tag="LAF92_002797"
     CDS             24706..25149
                     /locus_tag="LAF92_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBI9791780.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         6241 a         6516 c         6219 g         6223 t
ORIGIN      
        1 aacaggcctg taatagcagg cagataagaa tgagtgcagg tcgcttcacg actcttctcc
       61 agaggggcag aataaccaca ccggagtgtg gttatggtaa atcccttcat acttcaagcc
      121 acagatgcaa tgactcaaat tattttgggg atacatactt tattactggg tactggtggt
      181 cgtggttgtg gtggtcccgg tattagaacc atcaccgcca ccggttgccg cgagcgcgac
      241 acccaccgcc gagcttacgg tgctgacgct ggtcgcggag gaactccccg ccgaaaccga
      301 cgttgctgcc gaccctgccg cttcgcccac ctgtacaggt gcagcccacg cagaagtcgc
      361 cgcaagcgca gatatggcaa aaatgccata cagaactttt ttcataacaa tttcccttca
      421 ttgaatgaat ggagatttac ccaaaaacat ttcgggcggg atcgagtata cacaactaaa
      481 gcatgcgaat tacggcgcgg ggaattagag atggggtttt aggataattg gaatgaatat
      541 caaatataag ctacaactta tatataaata acattaaaaa tcataatgtt aaaataaaag
      601 taaaagtaat taatctgagt attttccgaa atcaatcacg attaaaaatc tattttagga
      661 ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct gataaaacaa
      721 tgccgacata acgtcggcat tgttattaag gtttttaaat taaccgcgcc acgctttata
      781 gcggttaatc agaccattgg tcgagctatc gtggctgctg atttctttgc catctttcag
      841 ctctggcaga atacggttcg ccagctgttt acccagctcc acgccccact ggtcgaaggt
      901 gaagatgttc agaatcacgc cctgagtaaa gattttgtgc tcatacagcg caatcaacgc
      961 ccccaggctg aacggagtga tttcgcgcag caggatggag ttggtcgggc ggttaccttc
     1021 gaacactttg aacggcacca cgtagtcaag cgttgccgga tctttaccct gatcgcgata
     1081 ttcctgctca accacttcgc gggatttacc aaacgccagc gcttcggtct gggcgaagaa
     1141 gttagacagc agtttctggt gatgatcaga gagcgggtta tgggtgatag ccggagcgat
     1201 gaaatcgcac ggtaccattt tggttccctg gtggatcagc tggtagaacg cgtgctgacc
     1261 gttagtgcct ggttcacccc agataatcgg gccagtctgg taatccacaa cgttaccgtt
     1321 acggtcaaca tacttaccgt tggactccat attgccctgc tggaagtacg ccgcgaaacg
     1381 gtgcatatac tggtcatacg gcagaatcgc ctcagtttcc gcaccaaaga aattgttgta
     1441 ccagatgcca atcagcgcca gcaggacagg caggtttttc tcggcaggcg tggtggagaa
     1501 atgcttgtcc atcgcgtgtg cgccagaaag cagttcaacg aagttatcaa agccgatgga
     1561 gagaacaatc gacaggccaa tcgctgacca caaagagtaa cgaccgccga cccagtccca
     1621 gaactcgaac atgttggcag tatcaatacc aaactcgcca acggctttgg cattggttga
     1681 gagcgccgca aagtgtttcg caacgtgttt ttcatcacct gccgctttca ggaaccagtc
     1741 acgcgcgcta tgggcgttgg tcatggtttc ctgagtggtg aaggttttag atgctaccag
     1801 gaacagcgtg gtttccgggt ttactttttt cagcacttca gcgatgtgag tcccatcgac
     1861 gttagaaaca aagtgcatgt tcaggtgatt tttgtacgga cgcagtgctt cggtcaccat
     1921 gtacggcccg aggtcagaac cgccgatccc gatgttcact acgtcagtga ttgctttgcc
     1981 ggtataaccc ttccactcac cggagataat cgcttccgag aaggttttca tcttctccag
     2041 caccgcgttg acttccggca ttacgtcttt gccatcaacc aaaatcgggg tattgctacg
     2101 gttacgcagc gctacgtgca gcacagcgcg gttttcagtg cggttgatct tctcgccaga
     2161 gaacatcgac ttaatcgcgc ccgccagatc gcactctttc gccagatctt gtaatttcgc
     2221 cagcgtctct tcagtgatgc ggtttttgga gtaatccacc agcatctgat cgccgaaggt
     2281 tgcggagaac ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga tcgtaacgtc
     2341 tttcatttca tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg ttggattgat
     2401 gtttttcatt agcaatactc ttctgatttt gagaattgtg actttggaag attgtagcgc
     2461 cagtcacaga aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt gtaaaccctt
     2521 agcgcagtga agcatttatt agctgaacta ctgaccgcca ggagtggatg aaaaatccgc
     2581 atgaccccgt cgttgacaac cgccccgctc accctttatt tataaatgta ctacctgcgc
     2641 tagcgcaggc cagaagaggc gcgttgccca agtaacggtg ttggaggagc cagtcctgtg
     2701 ataacacctg agggggtgca tcgccgaggt gattgaacgg ctggccacgt tcatcatcgg
     2761 ctacaggggc tgaatcccct gggttgtcac cagaagcgtt cgcagtcggg cgtttcgcaa
     2821 gtggtggagc acttctgggt gaaaatagta gcgaagtatc gctctgcgcc cacccgtctt
     2881 ccgctcttcc cttgtgccaa ggctgaaaat ggatcccctg acacgaggta gttatgtctg
     2941 aaattgttgt ctccaaattt ggcggtacca gcgtagctga ttttgacgcc atgaaccgca
     3001 gcgctgatat tgtgctttct gatgccaacg tgcgtttagt agtcctctcg gcttctgctg
     3061 gtatcactaa tctgctggtc gctttagcgg aagggctgga acctggcgag cgattcgaaa
     3121 aactcgacgc tattcgcaac atccagtttg ccattctgga acgtctgcgt tacccgaacg
     3181 ttatccgtga agagattgaa cgtctgctgg agaacattac tgttctggca gaagcggcgg
     3241 cgctggcaac gtctccggcg ctgacagatg agctggtcag ccacggcgag ctgatgtcga
     3301 ccctgctgtt tgtcgaaatc ctgcgcgaac gcgatgttca ggcacagtgg tttgatgtac
     3361 gtaaagtgat gcgtaccaac gaccgatttg gtcgtgcaga gccagatgta gccgcgctgg
     3421 cggaactggc cgcgctgcag ctgctcccac gcctcaatga cggcttagtg atcacccagg
     3481 gatttatcgg tagcgaaaat aaaggtcgta caacgacgct tggccgtgga ggcagcgatt
     3541 atacggcagc cttgctggcg gaggctttac acgcatctcg tgttgatatc tggaccgacg
     3601 tcccgggcat ctacaccacc gatccacgcg tagtttccgc agcaaaacgc attgatgaaa
     3661 tcgcgtttgc cgaagcggca gagatggcaa cttttggtgc aaaagtactg catccggcaa
     3721 cgttgctacc cgcagtacgc agcgatatcc cggtctttgt cggctccagc aaagacccac
     3781 gcgcaggtgg tacgctggtg tgcaataaaa ctgaaaatcc gccgctgttc cgcgcgctgg
     3841 cgcttcgtcg caatcagact ctgctcactt tgcacagcct gaatatgctg cattctcgcg
     3901 gcttcctcgc ggaagttttc ggcatcctcg cgcggcataa tatttcggta gacttaatca
     3961 ccacgtcaga agtgagcgtg gcattaaccc ttgataccac cggttcaacc tccactggcg
     4021 atacgttgct gacgcaatct ctgctgatgg agctttccgc actgtgccgg gtggaggtgg
     4081 aagaaggtct ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc tgcggcgttg
     4141 gcaaagaggt attcggcgta ctggaaccgt tcaacattcg catgatttgt tacggcgcat
     4201 ccagccataa cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggtg gtgcagaagc
     4261 tgcattttaa tttgtttgag taatatttcc ctactggcgt ataacgctca ccgattgtta
     4321 tacgccatcc tcactgaaaa catagattaa ataatatctg aaaagcaaaa tcactcctgt
     4381 agaacagtat tataatcacc gaaaaatatc atttaacagg agtgttaatg gctactttaa
     4441 tggaaaaaga tgcactttta aacggagcca gtcagtgcat cgcgttttta tcaaatatca
     4501 ttgataattg ctcagtttcg tcgcatcagg attcaggcga tgctctcaag cgattagtca
     4561 gctatcgcga ttatctttat tctacccccg ctgaacttgt tgattttaca caaggtaaga
     4621 ttctccttca gcaggtaaga actcaatatc agcatgaatt caacaatact acgcattcag
     4681 aaaataaagc atcttttgat tctatttggc agcgtctgac aaatcatgaa gtcacaccgc
     4741 aacagcatcc cattggtttt gttttaggtg ggcaacccgg agcaggtaaa tcatctctga
     4801 ttgaattagc caaaagagag acaaaagata atataatgat tattaacggt gatgattttc
     4861 gatttctaca ccctgatttt aattatatct accaaaatta tggggatgat tttgttaccc
     4921 atacagcgaa attttccggc gaaacggtcg aacgtgcaat tgagagagcg atagtcagta
     4981 aactcaacat tgttgtcgaa ggaacatttc gtaatgccgc gacgccgtta caaacattaa
     5041 aaaaactcaa agatgcaggc taccaaacag aagtcatgat aaaaacaacc tcagcggcat
     5101 taagctggga aagcaccaat gaacggtata gtaaggacaa agaggcggga aatattgccc
     5161 gaaaagtgga taaaaaccac catgatatcg tcaccggttt gctggcagaa aatgcccgca
     5221 aggtgtttgc ttcgaacctg tcagataagt ttgcggtgta ttcgcgtgag aagatgatat
     5281 ttagttcaca agcggcaacg aatgacgata ttgcaacact tatacagaat gaaatcagcg
     5341 ggaatacaca ataataataa gagctgatgg cgtgaaataa cgccgggctt tatgcccggc
     5401 gcattaccgt ctggtacatt aagctatcca gttccttaac gtccccttct ctgctatccg
     5461 gtaacagcgg tggaatttcc ccacgcgcct gttccgcacg aataaacgca tgtaacaatg
     5521 gacggcgcgg accatattcc gcttctcctg cgcgctgttt acgttccagt aattcgtcta
     5581 tttctgcacg taacggcgca tcaagttcgc ttcccgccag taactcagca aagcgcattg
     5641 gcggtacgcc tttacccgct tcgacccagc gcacggccag cagcggacgc agcacgtaaa
     5701 agtatttttt cagtcgaact tcgtcgccct gcaaatatcc ggggaagttt ttctgcgcca
     5761 tcgaatagta atgccagcgc gcacgcagcg gagaaaacca cattgggacc attgccttaa
     5821 acgccgttat cgtttcttca tcttgttgat aaatcaccgg agaatccagc cattctatca
     5881 gcgtcgggtt cgcgcctttc aataatccca gcgccttacg ccactcccaa ccggaaacat
     5941 ctaactcgtc gtcgatgggc agctcaataa catcgcgcgg tgactctact cgcagatacc
     6001 attccagcgg gtgaacatac aaaaaccgta catcgtagtc gctgtcgggc gaggcaaatc
     6061 cccagccgcg gctccctgat tcacaggcgt acagtacttt cacatcatac cgccgttcaa
     6121 tttcttttaa ctgctgcgat accctttcgc gcatcgcagc actgactcca tttaatggca
     6181 tgttactcac cctttgatat taaccaaccc atgcagaacc tgatccagac cgccaaatac
     6241 agagatgtta tcgatccgct cagccactct ctccagcgtt tctagctctt ttaaacgcaa
     6301 cgctaccgga ttgttctcca tgactttggc ggtgttcagt aacgagcgcg tcgctgccgt
     6361 ttcttcacgt cggcgaatca cattcgcctg cgcagatttc tctgcctcaa ccagctgcgc
     6421 gaggatattt ttcatatcac caggcaggac gatatctttc acccctaatg aggcaatctc
     6481 cataccaaat ggcaacatgc gcgatttcac ctgttcgctg acaacgtcat caatgacctg
     6541 tttatcttcc agcagttcat ccagcgtacg agtacctacc gcttcacgca gggcaaattg
     6601 caattcccga tacaaatgat caatcggctt cgttaactga ctaaatgcca gcaacacgtc
     6661 gctgtagcgc cagttagcgg cgaggttaat ccgcagattg accttatctt tcgttaagat
     6721 ttcttgcccg ctgacttcca gtacctgcaa gcgggtgtca accacttcag cgtcaaccag
     6781 atggttaatt ttccagtatg cggtcagccc cggcggtaac agcgcctgcg tttcaccatc
     6841 gattttcagc acgcctacat gccaggccgg aacctgcacc gttagtattg catcgcggcc
     6901 tttaactgcc cctttgcgcc gtggttgcaa taccgcattc atcacctccg tttgtacctg
     6961 gacttgctgc gtattcatcc gcaccagtgt caaatcatcc tctacacgcc agtacaaacg
     7021 gcgcgttgat ggcggcagga tctcctgcaa aataccgtcc atatacaacg ccccggcttc
     7081 aatctccgac aagtctgcca ccaggcaata tttctccacc caatcaggct gaaagcggcg
     7141 caaataatcc gccagcacgt cgggaacttc gctgccatcg agcgtaatca gcaagacttc
     7201 tggcgtattt aaccagggta aaagatgctc gcctgcatgt agcactttgt agtaatcgcc
     7261 gtttctggag agtaatgcca gctgatcttt tcttacgctt atttttttaa tcattatcta
     7321 ttccttgctt aacagccgtc gcggaagcag acacgaagcg acaggggaat tcccttttgc
     7381 ctgttacccg gcatctttat cggcgacgtt atcggggatc gctgactgcg caatccctca
     7441 ctttttattt aataagttaa aaccagtatt catttcagga atgaatgtgg gaatcgaacc
     7501 cataattggc gtcaaaggct gacggaacac gcccggaaaa cagcctggcc gttcttggtg
     7561 tgccgacggg gtttgacccc ctttgtaatt cagtttcaac gcgtacgaga tattgcgaga
     7621 agcgtgccag aattgagaaa aaattattaa ccaaatgaaa aataaagatt taataaatta
     7681 tttttgtttg tagagggaaa acttttcgct aaatttgtat ctgtatttat cgtcaattat
     7741 caagaaagat aaatatttaa gaagagaaca aaggaatatt atcagttcag ctcgcaaacg
     7801 aattcccgta cactgaactc tctggctcac tgagggcacc atcatggcac tcccccgtat
     7861 tacccaaaaa gagatgactg aacgcgagca gcgtgaactt aaaacactgc tggatcgggc
     7921 gcggattgcg catggtcgcg tactgaccaa ctccgaaacc aacagcatta agaaagagta
     7981 catcgataaa ttaatggtcg agcgtgaggc tgaagcgaaa aaagcccgcc agttgaagaa
     8041 aaagcaggct tataaaccgg atcctgaagc gtcatttagc tggtcagcaa atacgtcgac
     8101 gcgcggcagg cgctgattag cgccccttct ttttacgccc cggagaggta aaacgcttac
     8161 cgttggacgc cgggcgaccg cctttttccg ccgttttttc catcttaacg actggacgtt
     8221 tgatgcccgc tgttttcggt ttggccttcg ctttaggttt tgcctcggaa gaggaatttt
     8281 cgatgagctt aaagaggtcg attaactcat catcagttaa atcgcgccac tcacccagcg
     8341 gaatgccgct taaactgacg ttcataatgc gcgtacgttc cagctttttc acttcgtaac
     8401 caaagtgctc gcacatgcga cggatctgac ggttcagccc ctgcaccagg gtaatgcgga
     8461 agacaaacgg cgcttctttt ttgactttac actttttagt cactgtcccg aggattggca
     8521 cccccgcccc cataccacga ataaattcat cggtaatcgg tttatcgacc gtaaccagat
     8581 actctttctc atgatcgtta cccgcacgga ggatcttatt caccagatcg ccgtgattgg
     8641 tgaggaaaat cagcccctgg gagtctttat ccaggcggcc aatcgggaac acgcgtttgc
     8701 tgtggttaac gaaatcgaca atgttatcgc gctcgccatc ttcggtggtg ctgacaatac
     8761 cgacgggctt gttcagggcg ataagtacca aatcttcggc ttcccgaggc tcaatcaact
     8821 gaccgtttac tttcacaacg tcgccgggtt tcacctgatc gccaatggtg gctcgcttgc
     8881 cattaaggaa cacattgcct tgctcgatat agcgatccgc ttcgcggcgt gagcaaattc
     8941 cgctttcgct gatgtattta tttaaacgga ctgatgagtc gggcagcata aattctcctg
     9001 taaaaaacgg aatataccgt acctttgggt tgataaaaaa tagtcatgag cgaactattt
     9061 gttatttcca ttggcttgaa ttcgttacct gctcaaatca ctttccagaa gcaatgtgcg
     9121 acatcgtgag ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc ttccgtgatt
     9181 tcttactaac cggagaagta atggaactgc ttttattgag taactcgacg ctgccgggta
     9241 aagcctggct ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt cgccgctcag
     9301 cggtgtttat ccctttcgct ggcgtaacgc agacctggga tgattacaca gcgaaaacgg
     9361 ctgcggttct cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt gtcgatcccg
     9421 ttgccgcgat tgaaaatgct gagatcgtga ttgtcggcgg cgggaatact ttccagttgc
     9481 tgaaacagtg ccgcgagcgc gggctgctgg caccaattac tgacgtggtt aaacgtggcg
     9541 ctctgtatat tggctggagc gcaggcgcta accttgcttg cccaaccatt cgtaccacca
     9601 acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct aaatctgttc ccgttgcaaa
     9661 tcaacccgca cttcaccaac gcgctgccgg aaggccataa aggtgaaacc cgtgagcagc
     9721 gtattcgcga actgctggtc gtcgcgccag aactgacgat tattggtcta ccggaaggta
     9781 actggatcac agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc acttatgtgt
     9841 ttaaggctgg tgaagaagcg gtgccgctag aagctggtca ccgtttttaa gtgaattgcc
     9901 ggatgcatga tccggcatct tgttcatagt gcctgatgcg acgcttgacg cgtcttatca
     9961 ggcctacagg attcacaata ttaatactcg tcccgtcctt catcttcatc cggctgttcc
    10021 agcacactgt aagccaccga acaaaacagc gagttcaggc gctgcatatc tcccagtaag
    10081 cctaaatgta gcgaactggt ttcaatgctc tgcacgtttt gctgatgcag gcgatcgacg
    10141 tgggcgtggg aatagcggcg attaagaatg cgaaagcgat gcttactacg gcgcaaacga
    10201 cgagcgctgg tgacatcgcc agagaagaac accgacattg ccagttttaa attactgagc
    10261 aattgctcat agagcgcatc cagttccttc aagccatcaa gcgaaaatgc ccgccgtgct
    10321 gccagcgatt tatcggcaat ttcgctgccc atgcgctcga cgatatcgga agcctgttca
    10381 aggttgagcg acatttcaat aatctccgcc cagcgacgcg actcttcttc tgccagttcc
    10441 tcttttggca tccgcgccag atacagctta atggcggtat agagaacgtt gatatcatct
    10501 gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt attcagccct
    10561 tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgcgcggc gttcgccaga
    10621 gcaagcgtgg gcgtatccag cgcgctgaca tccagatgtt taggccgtag ctgggtatcc
    10681 agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc aacaaatggc
    10741 agcatgacca ggcaacgtac aaggttgtag aagacgtgga aatagatcac cagttccgct
    10801 tttggcaatg acaacttccc cattgtctct gccagcaaat ggacaaacgg caggataatc
    10861 aggctaccca ccagcttaaa cagcaaacta cccagcgcga cacggcgggc tgcggcattg
    10921 gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggttagcacc aatcaccaga
    10981 cagagcgcca cggggaagga gataatgcct gcggcggtca gagtcgcagt cagcagtaca
    11041 gcagcaaggc tggagtagct gataatggcg aacatcgcgc caatcagcgc atccagcaga
    11101 atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt ttgcctgggt gatcggcgtt
    11161 acggcctgca caatcaactc cagcgccagc aaaatcagcc caagaccaat accgacgcgg
    11221 cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc gataaaaata
    11281 agtaacggtg acagccagga taagtcgaag gtgagaatac gcgccattag cgccgtcccg
    11341 acatctgcac ccagcacaat gaccagagcc ggtgcgaggg ctaccagatc ctgagcgaca
    11401 aacgaggtca ccagcatggt ggtggcatta ctgctctgca ccagtgcggt aacgccgatc
    11461 cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt acgcaaacgc
    11521 gcgccgaaga cgcgcattac gccggttcga acaatatgag tcccccagac cagcagggcg
    11581 acggcagaaa gcaggtgaag cagcgttaac acgagttcag gttctcctta tcgttatatg
    11641 tgcctgaagc aattgaatga ccaaaggacc ggaaacacgc tgactggcat ctttactgtg
    11701 ccttttgaca ttcgtttttc cggctaagat tattccagcc tgtttccagt ataagtgttt
    11761 catattaata aagagacagg gcactcattg agcgccctgt ttgttgtaag gaaaagtgac
    11821 agatttgtga atcagtccgc gtcatacccc agattcggtg ccagccagcg ctcaacttcg
    11881 gtaacgctca tacctttacg gcgggcataa tcttcaacct gatcgcgctg aatttgtgct
    11941 acagcgtagt acttgctgtc cgggtggctg aagtaccaac ccgaaaccga tgcaccgggc
    12001 cacatggcga aagattctgt gagtttcatg ccagtgtgtt tttccacttc cagcagctcc
    12061 cagatggtgg ctttttccgt atgttccggg caggccggat agcccggtgc cggacggatg
    12121 ccctggtagt tttcgcggat cagttcttcg ttgctgaggt tctcgttcgg cgcatagccc
    12181 cagtagactt tacgcacacg ctcatggaga tactccgcaa aggcttccgc cagacggtcg
    12241 gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct gcgcttcaaa ggcatcagcc
    12301 agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc cgatgtaatc tgctttacca
    12361 gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag cgaatcccgt tttttcggtc
    12421 tgttgacgca gatggtggct gacgttgatc acatgggtgc gcgtttcgtc acggtagatt
    12481 tcaatgtcat cgcccacacg gtttgccggg aacaggccca ccacgccacg cggattcagc
    12541 gttttctcgg cgcttaattt atccagcatg tcgttggcgt ctttaaacag ccgctgcgcc
    12601 tcaacgccca ccacttcatc ttccagaatg cgcggatact tcccggccag cgaccaggtc
    12661 ataaagaacg gtgtccagtc gatgtaatta cgcagagttt cgatgctggc ttcaacttcc
    12721 tgcacgccga gacggtgcgc caccggcggc gtgtaagcct gccagtcaaa agcgaaatcg
    12781 ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgcg gtttcttacg cccgtgctga
    12841 atacgtacgg tttcgtactc cttgcgggta cgagcgacaa aatcatcacg ctgagtatcg
    12901 gaaagcagcg ccgccaccac accaacggtg cgcgatgcgt tctgtacata caccgtcggg
    12961 ccgctgtagt tctgctcaat tttcaccgcc gtgtgcgctt ttgaggtcgt cgcgccgcca
    13021 atcaatagcg ggatggtgaa gccctgacgc tccatctctt tcgccacgtt aaccatctcg
    13081 tccagcgacg gcgtgataag ccccgaaagt ccaatcagat cagcattcac ttctttagcg
    13141 gtacggagaa ttttttccgc aggcaccata acgccgagat cgacaatttc gtagttgtta
    13201 cattgcagca ccacgccaac gatattttta ccgatgtcgt ggacatcgcc tttcacggtg
    13261 gcgatcacca tcttgccgtt ggttttaccc tgctctttgc tggcttcaat aaacggttcg
    13321 agatacgcca ctgcctgttt catgacgcgc gccgatttga ccacctgtgg caggaacatt
    13381 ttcccttcgc caaacagatc gccgaccacg ttcatgccgt ccatcaacgg gccttcaatc
    13441 acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcgg tatcctgctc gataaactcg
    13501 gtaatgcctt tgaccagcga gtattccaga cgtttattca cttcccacga gcgccactcc
    13561 gcctgctggg cgttagcggt gtcgtcggtt ttgctgccgc gatatttctc ggcaagctcc
    13621 agtaaacgct cagtgccatc atcgcgacga ttaagaatca catcttccac cgcgtcgcgc
    13681 agttcagcgg gcaggtcgtc gtaaatcgcc agctgcccgg cattgacgat ccccatatcc
    13741 atgccattgc gaatagcgta gtagaggaac actgcgtgaa tggcttcgcg caccggatcg
    13801 ttgccacgga acgagaaaga aacgttagat acgccgccgg aaattagcgc gtgcggcagt
    13861 tcgcgtttga tgtcttcaca cgcgccgata aagtcctgcg cgtagttgtt gtgctcttca
    13921 atgccggtgg caaccgcgaa gatgttcggg tcgaagatga tatcttctgg cgggaagcca
    13981 acctcttcgg tgaggatttt gtacgcccga cggcaaatct cgattttccg tgcgcgggta
    14041 tcggcctgtc cctgttcgtc aaaggccatt accaccactg ccgcaccgta acgacgcaag
    14101 agcttcgcgt gatggataaa ggcgtcgacg ccctctttca tcgagataga gttaacaatg
    14161 cctttgccct ggatacactt cagacctttt tcaatgacat cccattttga ggagtcgatc
    14221 ataatcggca cgcgagcgat atccggttca ccggcaatca gattgagaaa acgcaccatc
    14281 gccgcttcgg cgtcgagcat cccttcatcc atgttgatat cgataatctg cgcaccgttt
    14341 tccacctgct ggcgggccac atccagtgcc tcgctgtatt tctcttcttt gatcaggcgc
    14401 ttgaacttag cggaaccggt gacgttggtg cgttcaccca cgttcacaaa caggctatct
    14461 tcgccaatgt tcagcggctc caggccggac aaacggcagg ctacggggat ttccggcagt
    14521 ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg caatatgttg tggcgtggtg
    14581 ccacagcagc cgccgacgat attgagaaaa cccgcttgcg cccattcacg tatctgtttt
    14641 gccatcgtgt cggcgtcgag atcgtactca ccaaaggcgt tgggtagccc ggcgttcggg
    14701 tgcgcggtga cgtagcattc cgcaatccgt gacagctcct gcacgtactg gcgcagttca
    14761 tcgggcccca gcgcacagtt caggccaaag gtcagagctt cggcgtggcg caatgagttg
    14821 taaaatgctt cggtggtctg cccggagagc gtgcgcccgg aggcgtcggt gatggtgccg
    14881 gagatcataa tcggcagctc aacgcccagc gcttcaaact ccgttttcac cgcaaaaacc
    14941 gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca ggatcagatc cgcaccacct
    15001 tccaccagcg ctttggtgga ttcgcgatag gccgccacca gctggtcaaa agtgatgttg
    15061 cggaatgccg gatcgttgac gtccggagaa atagacgccg tgcggttggt tgggccgaga
    15121 acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg tccactcgtc agcacaggcg
    15181 cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca gggattccat ctggtaatcc
    15241 gccatcgcaa tggtcgtgga gttaaaggtg ttggtttcga tgatatccgc gcccgcttca
    15301 aagtaggcgt tgtggatagc ggcgatcact tccggtttgc tgagcaccag caggtcgttg
    15361 ttgcctttga gatcgcatgg ccagtcggca aagcgttcac cacgaaaatc ggcttcgttc
    15421 agtcgatagc tctggatcat ggtgcccata ccgccgtcca gcaccagaat acgttcattt
    15481 aactgcgcac gcagttgttc cactttgctg ctcacacttg ctcccgacac gctcaaccca
    15541 gatttaataa aaattcaaca aaccatactg gcataaacgc atctgtggta aaagcgacca
    15601 ccacgcaaca tgagatttgt tcaacattaa ctcatcggat cagttcagca actattgcat
    15661 tagctaacaa taaaaatgaa aatgatttcc acgatacaga aaaaggaggc tgtcatggtc
    15721 gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg ccaccgcacc agcgactgga
    15781 caggttcagt ctttaacgcg tggcctgaaa ttactggagt ggattgccga atccaatggc
    15841 agtgtggcac tcacagagct ggcgcaacaa gccgggttac ccaattccac gacccaccgc
    15901 ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg tcggcgaact gggacattgg
    15961 gcaatcggcg cacatgcctt tatggtcggc agcagctttc tccagagccg taatttgtta
    16021 gcgattgttc accctatcct gcgcaattta atggaagagt ctggcgaaac ggtcaatatg
    16081 gcggtgcttg atcaaagcga tcacgaagcg attattatcg accaggtaca gtgtacgcat
    16141 ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga tgcacgcttc cggtgcgggt
    16201 aaagcctttt tagcccaact gagcgaagaa caggtgacga agctgctgca ccgcaaaggg
    16261 ttacatgcct atacccacgc aacgctggtg tctcctgtgc atttaaaaga agatctcgcc
    16321 caaacgcgca aacggggtta ttcatttgac gatgaggaac atgcgctggg gctacgttgc
    16381 cttgcagcgt gtattttcga tgagcaccgt gaaccgtttg ccgcaatttc tatttccgga
    16441 ccgatttcac gtattaccga tgaccgcgtg accgagtttg gcgcaatggt gattaaagcg
    16501 gcgaaggaag tgacgctggc gtacggtgga atgcgctgac tttttctggc gggcagaggc
    16561 aatattctgc ccatcatacc tgagtggcaa tagaataagg gtgtctgtta atcgtattga
    16621 cgccagaata acttaatgtc atacacttca cacactttta ttaactcggc attattttta
    16681 aacatcaaac cactttaatt attaacaaaa tcgcaagatc tcattccgcc gaaactttta
    16741 cggtttcatt atgagattga gcgatttatg atatttcttt acttgtaatc atattaaatt
    16801 ttttcactgc tctgatagtt aaaattcaag acatcaataa acaatgagat atttaaatga
    16861 ttactcgtat ccctcgtagt tctttctctg caaatattaa taatacagcc cagacaaatg
    16921 aacaccaaac cctgagtgaa ttgttttata aagaactcga ggataaattt tctggcaagg
    16981 agctggcgac gcctctatta aaaagcttct cagagaactg tagacaaaat ggtcgtcata
    17041 tctttagcaa caaggatttt gtcattaaat tttccacgtc cgtcttacaa gctgataaga
    17101 aagaaattac gataattaat aaaaatgaaa acacgacact cactcaaacc attgctccaa
    17161 tatttgaaga atacctaatg gaaattttac ctcaacgctc agacactctt gataaacaag
    17221 aattaaacct aaactcagtt agaaaagaaa aagaattccc aagagttaaa cttaatgatc
    17281 aatgttattt tccggggcgc ccccaaaacc gtatagtatg ccgacacatt gctgcacaat
    17341 atattaatga tatttatcag aatgttgatt acaaacccca tcaagatgat tactcttcag
    17401 ctgaaaaatt tctcacgcac ttcaacaaaa aatgcaaaaa ccagactttg gcgttggttt
    17461 ccagccgtcc tgaggggcgt tgcgttgctg cctgcggtga tttcgggcta gttatgaaag
    17521 catattttga caagatggaa tcaaatggca tcagtgttat ggcagccata ttactggtgg
    17581 ataaccatgt tttgacggtc cggctaagaa taaagaacac aactgaagga tgtacccatt
    17641 acgtggtttc ggtttatgat cctaatgtca ctaacgataa aataagaatt atgagcgaaa
    17701 gcaaagagga tattaaacac tattctctga tggattttat gaatgtagat tatagcctcc
    17761 tgaaatggtc aaatgatcat attattaacc aatctgttgc aataattcca gcacttccga
    17821 aagaacagct attgatgtta aaaggatctg tggatgaaat aacccctcca ttatcacctg
    17881 caacgatgaa tttgctaatg gcaattggtc agaatcacca acttacgcaa ctgatgattc
    17941 aactccagaa aatgccagaa ctacatagaa cagaaatgtt gactgcctat aatagtggac
    18001 atatgaacgt tattaatact atttttaacg cattacccac tctttttaat acgtttaaat
    18061 tcgataaaaa aatatgaagc ccctcctcct ggcaaataat tctaatgaat atcccggttt
    18121 gttttcagcg atacagcata aacaacaaaa cgttgtagag acggtttatc ttgctttatc
    18181 tgaccatgca cgcctgtttg gatttaccgc tgaagatatt atggattttt ggcaacacaa
    18241 agcgccacaa aaatactctg cctttgagtt ggcttttgaa tttggtcacc gggttattgc
    18301 tgaattaatc cttaatacat taaataagat ggctgaaagc tttggcttta cggataaccc
    18361 tcgatacatt gcggagaaaa attatatgga agctttactc aaaaaagcat ctccccatac
    18421 cgtacgctaa atctttgcct gcgccgatac gcataaacat cttccacatg cccttcacgt
    18481 atgcggtttt gtagtgcgcg ccagtaatca gcgcggaaca ggtcggcgtg catctcttca
    18541 aacagcggac caatacgcgg gtcggcgcat agccagtggc gaaactcttc cgggaaaaca
    18601 tcgcccggcg agacgctgta ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc
    18661 ggcgggatgt cgcggaaatt cacttccgtc atgtagcaaa tttcatcgta atcataaaaa
    18721 accacacgcc cgtgacgggt gacaccgaag tttttaaaca gcatgtcgcc aggaaaaatg
    18781 ttagcagcgg caagctggcg aatagcgtta ccgtattctt caatggcgtc gcgcaactgc
    18841 tgaccttcca cttgttccag ccagatattg agcggcacca tccgccgctc gatatagaga
    18901 tggcgaatta caatctgctc accaagatcg gtgatttttt ccgctgcttc ctgaagcagt
    18961 aattccatta atgccgggga aatatgccgc ttctccagca caaagttttc aaactcctgg
    19021 gtgtccgcca ttcggcccac acggtcgtgc tctttcacca gttgatagca ggcacgaacg
    19081 tgagcagcag acatctcttt ctgcggcgcg aacttgtctt tgatgacttt gaatacccga
    19141 tcaaagcccg gcagcgtaaa caccaacatc accattccac gaatacccgg cgcttcaatg
    19201 aactgctcat tacagccctg tagataaacg agatattcgc ggtagctttc ggttttggcg
    19261 tgcttctggc agccgatagc catatacaat tcagcagtgg ttttacctgg cagaatttcc
    19321 cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa ccataaaata agaacgcgca
    19381 aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc aggtatcaat aaataactcg
    19441 ccgtcgtccg tctggtggat cggcagtaag aagggcaacg tgccggaagg cgtgatcagt
    19501 ttgcccacca gccaggcggc tttattgcgg taaaacagtt cgttcgccac ctgtaaatga
    19561 ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat ggcgaatgat gtaatgaatg
    19621 tcacggcttt tattctgcca gcgcaggcgc agcggcaggt cgctgataac gcgcatcagt
    19681 agagattccc agccgtgatc ggggtgaaag tctttcgcca gcgggcgggg aatggtacga
    19741 aagcggcgct ctggctgaga gctaaagata aaaagccgct cgggagtaag cgagcggtgg
    19801 tcaaataacc gacagtacac ggagttaaaa aagctctccg caatctcgaa gcgcgggtaa
    19861 tccggcaaca gccgcgtgta atgctcttta acgcgcagta aaaatgccgc gtccgtactt
    19921 tggccgttag taatgcagcg cagttgctcc acgaccagac caacgtgatg atcgtaaaga
    19981 tggatacggt ttttcatcgc ctgctggaca gcatgccagt cggcctgttc gaaacgctgc
    20041 tgcgccccgg aagtcacttc gaggaatcga ccatactgag cgtcgaagcc ttgcaaaatg
    20101 gtttgagcaa tcagtaattc caggccacgc ggcatttagc gccctcatca ggagcagaga
    20161 attgccggat gcggcgtaaa cgccttatcc agcctacgtt cggcaacggt tgtaggcatg
    20221 ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc ggcgtaaatg
    20281 ccttatccgg cctacgttcg gcaacggctg taggcatgat aagacgcgca agcgtcgcat
    20341 caggcatcgt gcaccgattg ccggatgcgg cgtgaacgcc ttatccggcc tacagtcagc
    20401 aacggttgtt gttgcttaga actgcgattc ttcagtggag ccggtcagcg cggtgactga
    20461 agacgtgccg ccctgaataa tagtcgtcac tttatcgaag taacctgtac ccacttcctg
    20521 ctggtgagat acgaaggtat agccatcttt cgcggcggca aattccggct gctgcacttt
    20581 ctcaacgtag tgcttcatac cctcgccctg ggcataggcg tttgccaggt caaacatgtt
    20641 gaaccacatg ctgtggatac ctgccagggt gatgaactgg aacttgtagc ccatatccga
    20701 cagctgctgc tggaagctgg caatagtttt gtcgtcgagg tttttctgcc agttgaacga
    20761 cggcgagcag ttataagcca gcagtttgcc cggatatttc gcgtggatag cttgtgcaaa
    20821 gcgacgcgcc agttccagat ccggcgtgga ggtttcacac cagaccaggt cagcatatgg
    20881 cgcatacgcc aggccacggc tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc
    20941 ttcactggta cgctcgccag taataaattc actgtcatac gggtcgcaat cggaggtaat
    21001 cagatccgcc gcatcggcat cggtacgagc aaccagcagg gttggaacgc ccgtcacgtc
    21061 agctgcaaga cgcgccgcga ccagtttctg aatagcttcc tgagttggca ccaaaacttt
    21121 gccgcccatg tgaccgcatt tcttcactga cgccagctga tcttcgaagt gaactgccgc
    21181 tgcaccggct tcaatcatcg ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc
    21241 ggcttccgca tcggcaacga tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc
    21301 aatgcctgcg gaccattgga tctgatcggc acgacggaag gtgttgttga tccgctccac
    21361 cacagctggc accgagtttg ccggatagag cgactgatcc ggatacatgc tggccgccag
    21421 gttagcgtcc gccgctacct gccatcccga cagatagact gcttcaatac ccgctttcgc
    21481 ctgttgcagc gcctgaccgc cagtcagtgc accgaggctg ttgatgtagc cttttttcga
    21541 ctcgccatgc agcagacgcc acattttcgc cgcgcccagt tgcgccaggg tgcattcagg
    21601 attgactgaa ccgcgtaatt tcaccacatc ttccgcgctg tatgggcgag taatgccttc
    21661 ccaacgcggt tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat
    21721 gtgcagatgc tccatagtta tgtggtggtt tacgctaaca ggcggtagcc tggcagggtc
    21781 aggaaatcaa ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca
    21841 aaacgcccct gggagaaacg ttcttcgccc agttcgctgg caatgacttt catctcttca
    21901 cccagcatct ggcggaacaa ggctttggtc accggtttgc cattgctcaa cgttttttga
    21961 tgatggatcc actgccagat tgaggtacgg gaaatttcag ccgtcgccgc atcttccatc
    22021 aggccataaa tcggcacgca gccgttgccg gagatccatg cttcgatgta ctgcactgcc
    22081 acgcgaatgt tggcgcgcat accttcttcg gtgcgttcac catcacaagg tgccagcagc
    22141 tgatcggcag taatcggcgc gtcttgttcg cgcatcactt caagctgatt tttacgggag
    22201 ccgagaatgt cgttgaatac cgccattgcc gtatccgcaa ggcctgggtg agcaatccat
    22261 gtgccatcgt gaccgttatt ggcttccagc gctttatccg cttttacttt gttgagcacc
    22321 tggttattgc gctcttcatc tttgctcgga ataaacgctg ccatgccgcc catcgcaaaa
    22381 gcaccgcgtt tatggcaggt tttaatcaac aggcgtgagt aagcattcag gaatggttta
    22441 tccatcgtca ctgcctgtct gtctggcagg acgcgatcgg gatagttttt caacgttttg
    22501 atatagctga agatgtaatc ccaacgaccg cagttcagac caacaatatg gtcacgcagc
    22561 gcgtgaagga tttcatccat ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc
    22621 ttgatggtgc cgcgcggcag attaaagcga tcttctgcat agctgaagac ttcgctccac
    22681 caggccgctt cctgccagga ctgggttttc ggcagataga aatagggacc gctgcccttt
    22741 gccaacagtg cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag gctaccgggg
    22801 attgcctcac cacgccaggt gacatgtttt tccggcaagt gcagaccgcg tacccgacaa
    22861 atcaaaaccg ctggattggg cttgagctga taaattttgc ctgcttcatt ggtatagctg
    22921 atggtgccgt taaccgcatc gcgcaggtta atttgcccgt cgatcacttt gttccagtct
    22981 ggtgccagtg aatcttcgaa atcggccata aagactttca cattggcgtt cagtgcgttg
    23041 atcaccatct tgcgctcaac cgggccagtt atctcgactc gacgatcttg taagtccgcg
    23101 ggaataccac gaattttcca gtccgtatcg cgaatggaag ctgtttccga aataaaatca
    23161 ggcaacgttc cgttatcaat atcttgctgc tgctgaatgc gcgctgccag aagtttattg
    23221 cgttgtggcg taaaatgcgt caccagctca gtcagaaatt ctaccgcttc ggcagtaaga
    23281 atttgcttct cctgctcgcc atacggcctt atgaaagcca gttcatcggt tgttgttgcc
    23341 tgttcagtca tcgtgcagct cctcgtcatg gatccgaaaa cttccccact gtgaacgaag
    23401 gatcgttgtg cgcttttcgt tcagcacaac taagactact catttaaaat gcaaaatcaa
    23461 aaacaatttc cattttaatt aaaaatactt gataacttat tgataacaat atgaataaaa
    23521 tttattcacc tcctgaggtg cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt
    23581 aaggtgctga atcgcttaac gatcgactat cacagaagat taatccagcg ttggattcat
    23641 gtgccgtaga tcgtatggcg tgatctgata gacgtaatag ttgagccagt tggtaaacag
    23701 caaattaccg tgactacgcc agctcgctcg cggtgtattt tgcggatcat tgtgcgggaa
    23761 atagttatac ggtacttccg ggcctagtcc ggcttccaca tcgcggaaat attcctgcgc
    23821 cagcgtttgc gcatcatatt cgggatggcc cgtcacaaag gcaatgcgct tatctttgct
    23881 ggcaaacaga tatgcatccc cttcttccgt ctctgccaga atttccagat cggtgtaatc
    23941 acgaatcaac gctgccggaa agtcagcata gcgcgaatgt ggtgccagga atgaatcatc
    24001 aaagccacgc gtcagaagcg catgaggatg gagaatatga tgctcgtaaa cgccagagag
    24061 tttgtcggtg cgagtttgct taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc
    24121 ccagcagaca aacagcgtcg aggtgacgtg atctttcgac cactccagca cctgtttgat
    24181 ctgcggccag taagcgacat cattaaactc caccaggccc agcggcgcac cggttacaat
    24241 cagaccgtca aagttctgtt cctgaatatc ttcaaagtta cagtagaagt tgttcagatg
    24301 ctctgcgggc gtgttgcgcg attcacggga atcgatgcgc aacagctgaa tatcgacctg
    24361 caaaggtgag tttgaaagca ggcgcagaaa ctgattttca gtttcaatct tcttcggcat
    24421 caggttaagg atcagaacct taagtggacg aatttcctga ccagacgcac gagaagttgt
    24481 catcacaaag acgttttctt cacgcaagaa attgacggcg ggtagctcgt ccggcacacg
    24541 aatcggcata acctgattac ctcactacat acgcttatac gtttagacat ccagatagct
    24601 gaagataacc agaaaatttg ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa
    24661 ggaaaagact acgttagaat ataagaatga cgaaaaggag agaaaatggt tattagtatt
    24721 cgccgctcac agcatgagga aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat
    24781 gccacccacg attttttatc agcagagtat cgggccgagc tggaagagct ggttcgttcc
    24841 tttctgccgg aagcaccgtt gtgggtcgcg gttaatgaac gggatcagcc ggttgggttt
    24901 atgttgttaa gtgggcagca tatggatgcg ctgtttatcg atcctgatgt gcgcggctgc
    24961 ggcgtgggtc ggatgctggt ggagcatgcg ctctcgatgg caccggaact gacaaccaac
    25021 gttaatgagc aaaatgagca ggcggttggg ttctataaga aggtgggttt taaggttacg
    25081 ggacgctctg aggtggacga tttggggaaa ccgtatccgt tgctgaatct ggcgtatgtg
    25141 ggggaataag ccgcccctgc cgtgcctgcc caggctggag aagacgcgcc agcgtcgca
//
LOCUS       DAEVZA010000069        24653 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEVZA010000069 DAEVZA010000000
VERSION     DAEVZA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.069"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..442
                     /locus_tag="LAF92_002798"
     CDS             <1..442
                     /locus_tag="LAF92_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBI9791781.1"
                     /translation="PVLILTARDALEERVEGLRLGADDYLCKPFALIEVAARLEALMR
                     RTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLELLMRNAGRVLPRKLIE
                     EKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK"
     gene            439..1788
                     /gene="qseC"
                     /locus_tag="LAF92_002799"
     CDS             439..1788
                     /gene="qseC"
                     /locus_tag="LAF92_002799"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBI9791782.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(1834..2166)
                     /locus_tag="LAF92_002800"
     CDS             complement(1834..2166)
                     /locus_tag="LAF92_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBI9791783.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            2485..3066
                     /gene="mdaB"
                     /locus_tag="LAF92_002801"
     CDS             2485..3066
                     /gene="mdaB"
                     /locus_tag="LAF92_002801"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBI9791784.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            3097..3411
                     /locus_tag="LAF92_002802"
     CDS             3097..3411
                     /locus_tag="LAF92_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBI9791785.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(3459..5351)
                     /gene="parE"
                     /locus_tag="LAF92_002803"
     CDS             complement(3459..5351)
                     /gene="parE"
                     /locus_tag="LAF92_002803"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBI9791786.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(5380..5961)
                     /gene="yqiA"
                     /locus_tag="LAF92_002804"
     CDS             complement(5380..5961)
                     /gene="yqiA"
                     /locus_tag="LAF92_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBI9791787.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(5961..6788)
                     /gene="cpdA"
                     /locus_tag="LAF92_002805"
     CDS             complement(5961..6788)
                     /gene="cpdA"
                     /locus_tag="LAF92_002805"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBI9791788.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(6813..7235)
                     /locus_tag="LAF92_002806"
     CDS             complement(6813..7235)
                     /locus_tag="LAF92_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBI9791789.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(7236..7865)
                     /gene="nudF"
                     /locus_tag="LAF92_002807"
     CDS             complement(7236..7865)
                     /gene="nudF"
                     /locus_tag="LAF92_002807"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBI9791790.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            8070..9551
                     /gene="tolC"
                     /locus_tag="LAF92_002808"
     CDS             8070..9551
                     /gene="tolC"
                     /locus_tag="LAF92_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBI9791791.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            9699..10370
                     /locus_tag="LAF92_002809"
     CDS             9699..10370
                     /locus_tag="LAF92_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBI9791792.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            10376..11536
                     /locus_tag="LAF92_002810"
     CDS             10376..11536
                     /locus_tag="LAF92_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBI9791793.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(11574..12362)
                     /gene="ygiD"
                     /locus_tag="LAF92_002811"
     CDS             complement(11574..12362)
                     /gene="ygiD"
                     /locus_tag="LAF92_002811"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBI9791794.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKENLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            12505..13278
                     /gene="zupT"
                     /locus_tag="LAF92_002812"
     CDS             12505..13278
                     /gene="zupT"
                     /locus_tag="LAF92_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBI9791795.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(13336..13506)
                     /locus_tag="LAF92_002813"
     CDS             complement(13336..13506)
                     /locus_tag="LAF92_002813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791796.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(13767..14420)
                     /gene="ribB"
                     /locus_tag="LAF92_002814"
     CDS             complement(13767..14420)
                     /gene="ribB"
                     /locus_tag="LAF92_002814"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBI9791797.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(14524..14672)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            14794..15084
                     /gene="ubiK"
                     /locus_tag="LAF92_002815"
     CDS             14794..15084
                     /gene="ubiK"
                     /locus_tag="LAF92_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBI9791798.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            15367..15918
                     /locus_tag="LAF92_002816"
     CDS             15367..15918
                     /locus_tag="LAF92_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HBI9791799.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            15978..18482
                     /locus_tag="LAF92_002817"
     CDS             15978..18482
                     /locus_tag="LAF92_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI9791800.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            18498..19247
                     /locus_tag="LAF92_002818"
     CDS             18498..19247
                     /locus_tag="LAF92_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI9791801.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            19249..20313
                     /locus_tag="LAF92_002819"
     CDS             19249..20313
                     /locus_tag="LAF92_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9791802.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(20356..20556)
                     /gene="glgS"
                     /locus_tag="LAF92_002820"
     CDS             complement(20356..20556)
                     /gene="glgS"
                     /locus_tag="LAF92_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBI9791803.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            20825..21454
                     /locus_tag="LAF92_002821"
     CDS             20825..21454
                     /locus_tag="LAF92_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HBI9791804.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            21481..23142
                     /locus_tag="LAF92_002822"
     CDS             21481..23142
                     /locus_tag="LAF92_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBI9791805.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            23381..23440
                     /locus_tag="LAF92_002823"
     CDS             23381..23440
                     /locus_tag="LAF92_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI9791806.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            23757..23816
                     /gene="ibsE"
                     /locus_tag="LAF92_002824"
     CDS             23757..23816
                     /gene="ibsE"
                     /locus_tag="LAF92_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBI9791807.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(23936..>24653)
                     /gene="rfaE2"
                     /locus_tag="LAF92_002825"
     CDS             complement(23936..>24653)
                     /gene="rfaE2"
                     /locus_tag="LAF92_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HBI9791808.1"
                     /translation="LQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEA
                     CFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAAARKRGEK
                     VVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIV
                     LGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLN
                     FEDGCSTTNIIKKIQQDKKG"
BASE COUNT         6647 a         6109 c         5787 g         6110 t
ORIGIN      
        1 gccggtactg atcctgaccg cgcgcgatgc gctggaggaa cgtgtagaag ggctgcgtct
       61 gggagctgac gattatctgt gtaaaccttt tgcgttgata gaagtcgccg ccaggctgga
      121 agctctgatg cgccgaacca acggccaggc cagcaacgag ctgcgccacg gtaacgtcat
      181 gctcgacccc ggcaaacgta tcgccacgct ggctggcgaa cccttaacac tgaaaccaaa
      241 agaatttgcc ctgctggaat tactgatgcg taacgctggt cgggtactgc cgcgcaaact
      301 gattgaagag aaactgtata cctgggacga agaggtcacc agtaatgccg ttgaagtgca
      361 tgtgcatcat ctgcgacgca aactcggtag tgattttatt cgtaccgtgc atggtattgg
      421 ctacacatta ggtgagaaat gaaatttacc caacgtctta gtctgcgcgt caggctgacg
      481 ctaatctttt taattctggc ctcggtgacc tggctgcttt ccagctttgt cgcctggaaa
      541 caaacaacgg ataacgtcga tgaattgttc gacacccaac tgatgctgtt tgccaagcgg
      601 ttaagtacgc tcgatctcaa cgaaatcaac gcggcggatc gcatggcaca gacgccaaat
      661 aaattaaaac acggtcatgt tgatgacgat gcgctgacct ttgccatctt tacccacgac
      721 ggcagaatgg tccttaacga tggcgataac ggagaagata ttccttatag ctatcaacgg
      781 gaaggttttg ctgacgggca actggtgggc gataaagatc aatggcgttt tgtctggatg
      841 acctcacctg atggcaaata tcgcatcgtt gttggtcagg aatgggaata ccgtgaagac
      901 atggcgctgg cgattgttgc cgggcaattg atcccgtggc tggtcgcact gccgattatg
      961 ttaatcatca tgatggtact attgggtcgt gaactcgcgc cgctgaacaa actggcgctg
     1021 gcgctgcgta tgcgtgaccc tgactcggaa aaaccactaa acgcgactgg cgtacccagc
     1081 gaagtgcgtc cgttggttga gtcgctaaat caactgttcg cccgcacaca tgcgatgatg
     1141 gttcgtgaac gacgctttac ctccgacgca gctcacgaac tacgtagccc gttaacggcg
     1201 ctgaaagtgc aaaccgaagt tgcgcagctt tctgacgatg atccgcaagc gcggaaaaaa
     1261 gcgctgcttc aattacatta cgggatcgat cgcgctactc gtctggttga tcaactgctc
     1321 actctatcgc ggctggactc actggataac cttcaggacg tcgcggagat cccgcttgaa
     1381 gatctcctgc aatcgtcggt gatggatatt taccacacgg cgcagcaggc gaaaattgac
     1441 gtgcgactga cactcaatgc ccacggcatc aaacgcaccg gacaaccgct attgctaagt
     1501 ttgttggtgc gaaatttgct ggataacgcc gtgcgctaca gtccacaggg cagcgtgata
     1561 gacgtcacgc tgaatgctga taatttcatc gtgagggata acggccccgg tgtgacgcca
     1621 gaggctctgg cgcgaattgg cgaacgcttc tatcgccccc ccggacaaac cgctaccggc
     1681 agtggacttg ggctatcgat tgtccagcga atcgccaaat tgcatggcat gaatgttgag
     1741 tttgggaatg cggaacaagg tggatttgag gcgaaggtaa gctggtaact tctctgtcta
     1801 tgagagccgt taaaacgact ctcatagatt ttactaatag caaaatataa accgtcccca
     1861 aaaaagccac caaccacaaa ccagacaaat accgagcgaa aatacatata cccaaaactc
     1921 gaacatttcc cgcataaaga gttttcttaa gataagaata ataagtggcg taagaagaaa
     1981 aaatgctgca atcattgctt gcaatatacg atcttcgcct gaaacggaag gtatactgca
     2041 aatattgtct cccgactcaa gcatccagtc atacttattg aaagcaaaaa aagcatctat
     2101 taaccaataa gtaagcatta tgtataacag agcttcaaat attgtttgaa ttttatgctt
     2161 taacatatat cagtacgctc ataaaaatcc atttataatt atcaacatga cacgttgata
     2221 ttttcatctt ttacttattc aaagccatat tttcaatccc aaaaaaaacc gaaccaaacg
     2281 tgtaaattat tccggtgcga gtaagaatta tgaggaatgg ctatcagtat tgtcattttc
     2341 agaaaatatt tatcctgcat cggtgagtca gagctagatc agacttttgc taaattcgca
     2401 aaagactttg cacattttgc taatttcacc gtaccgctct gtgacgtact atagtcgaca
     2461 aacgtctcac cttgaggtta aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa
     2521 ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg
     2581 cgcgacctcg ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg
     2641 gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg
     2701 ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat tcaccgaagg tcatggaacg
     2761 ctgtatgcca gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc
     2821 ctggtacagg gcaaaaaata tatgctttct ctgacctgga acgcaccaat ggaagccttc
     2881 accgaaaaag atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat
     2941 aaagcaaacc aattccttgg catggaaccg ctgccgacat ttatcgctaa tgacgtgata
     3001 aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt
     3061 ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc
     3121 cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt
     3181 ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct
     3241 ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc
     3301 atcgcgcatc ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta
     3361 aaaggcgacg tgctggagat gaatatccgc atcctgcaac ctgggattta aacctgcctt
     3421 gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg
     3481 tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcaacata
     3541 tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg
     3601 cgagtgttcg gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct
     3661 ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct
     3721 ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt
     3781 ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa
     3841 gcgcagagca gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt
     3901 ttgccataac gaagctggct cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa
     3961 atatcatgca cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg
     4021 atcttacctt tcagtggcat gatcgcctga tattcgcgat cgcgcgcctg cttggcagat
     4081 ccgcctgcgg agtcaccttc cacaaggaac agttcggtac ggttaagatc ctgcgcggta
     4141 cagtcagcca gtttgccagg cagtgctggg ccgctggtca gctttttgcg caccactttt
     4201 ttagccgcac gcatacggcg ctgggcgctg gaaatcgcca tctccgccag caactccgcc
     4261 gcctgaacgt tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacaaat
     4321 gccgcgcact gacgcgaaga gagacgctct ttggtctgcc cggcaaactg cggatcctgc
     4381 atttttactg acagcacata ggcgcagcga tcccagatat cttccgccga caactttaca
     4441 ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccaa caggccctga
     4501 cgcagaccgt taacgtgcgt accgccctgc atcgttggga taaggttgac gtagctttcg
     4561 gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg
     4621 ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc
     4681 gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca
     4741 tctttaaagg taatctcaac gccggggcac agtactgctt tggccttcag cacatgcgtc
     4801 aggcgtgaaa cagaaaaacg cgggctgtca aagaaggttt catccggcca gaagtgcacg
     4861 ctggtaccgg tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg
     4921 ccattttcaa aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttctacgcgc
     4981 ttcgacaggg cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa
     5041 tttttgttag agaatttacc gcctgcatgc agacggcaaa gaatcagttc aaccgccggt
     5101 acaccctctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat aacttctaac
     5161 gactggtcag catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg
     5221 acactgttat caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc
     5281 cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa
     5341 gtttgcgtca tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag
     5401 aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc
     5461 tatgacagtc tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc
     5521 atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat
     5581 gactttgaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt
     5641 ctcgttctga ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac
     5701 cactgcgggc agcataaaac attgtgacaa ccaggtggcg taatatcccc ccagtgacga
     5761 accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca gcagctctgc
     5821 cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc
     5881 cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg
     5941 taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg
     6001 tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc
     6061 cagccgggcg cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag
     6121 gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata
     6181 tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc
     6241 gcgttacgca gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc
     6301 agcagcagca acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc
     6361 tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt
     6421 tgccattgct caccaataaa cacgcgcttc gccggggaga tacccgcatc ctgtaacgcg
     6481 ctgtacatcg cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa
     6541 cttgcgatgc cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca
     6601 cctgtcgcga caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc cagcaccgcc
     6661 tggtagcttt cccaggtgtt tacccctaac agggcttcgt gcttttgtgc aaacaggtga
     6721 gtatcggtaa tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg
     6781 ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc catcgctcca
     6841 tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg cttttcgtcg
     6901 cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg
     6961 cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg
     7021 ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc
     7081 gattccacaa tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg
     7141 ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc
     7201 atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct
     7261 tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc
     7321 tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt
     7381 tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt
     7441 gacgaacgct cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt
     7501 ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca
     7561 ccctcttcta tcatcccggc aaccatctcc agtagccagg gggtttcact ggtgtcgtat
     7621 gcggcaatcc gaatctgctc aatcagcaca acttcatcac gcactgggtc aaagggtagc
     7681 aagactgcgg cgtgaccgcg ctcaaaaatt tccctccgta cctcatgact catttgcccg
     7741 ttgaataaac gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt
     7801 tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta
     7861 agcattgtta atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac
     7921 tgccgtttga gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc
     7981 caaccttttg cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc
     8041 taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc
     8101 tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc
     8161 aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg
     8221 aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca
     8281 cctatagcaa cggctaccgc gacgcgaacg gcatcaactc gaacgcgacc agtgcgtccc
     8341 tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa
     8401 aagctgcagg gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca
     8461 ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac
     8521 aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg
     8581 tagcgatcac cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag
     8641 tgaccgcacg caataacctt gataacgcgg tagagcagct gcgccagatc accggtaact
     8701 actatccgga actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg
     8761 ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga
     8821 gccaggacct ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgacgctgg
     8881 atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg
     8941 ccgctggtac ccagtatgac gacagcaata tgggccagaa caaagtgggc ctgagcttct
     9001 cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggca cagtacaact
     9061 ttgtcggtgc cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt
     9121 cctccttcaa caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag
     9181 tttccgctca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca
     9241 ttgttgatgt gttggatgca accaccacgc tgtacaacgc taagcaagag ctggcaaatg
     9301 cgcgttataa ctacctgatt aatcagctga atattaagtc agccctaggt acgttgaacg
     9361 agcaggatct gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa
     9421 acgttgcccc gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc
     9481 cggcacccgt cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt
     9541 tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga
     9601 tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta
     9661 ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac
     9721 gccacgcatc gttccgcaaa aactggagcg cgcgccatct gacaccagtc gctctcgcgg
     9781 ttgccactgt ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc
     9841 aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt
     9901 acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact
     9961 gtgttgctga atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac
    10021 cagaaaacca ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg
    10081 gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct
    10141 cctcgaaaaa cccagccagc ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact
    10201 atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac
    10261 cggcgaccac cactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca
    10321 ctatgcagcg tagcgcaact ggcacctctt ctcgttcaat gggtggctga taccgatgga
    10381 aagagtcagt attaccgagc gcccggactg gcgtgagaaa gcccacgaat acggtttcaa
    10441 ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct
    10501 cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt
    10561 ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacacac
    10621 ctggagtttt gtgcgccagt catggctgac gcaccagcca tcgctttatt cgcgtcttga
    10681 tctggcgtgg gatggcactg gcgaacctaa acttctggaa aataacgccg atacgccaac
    10741 gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg
    10801 taacttgccg gagggcagcg accagtttaa cagtctgcaa gaaaaactga tcgatcgctt
    10861 cgttgagctg cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac
    10921 ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcaacggaag ctgaaattgc
    10981 tactgagttc ctctacatcg atgatatcgg gttaggtgaa aaaggtcagt tcacggattt
    11041 acaggatcag gtaatttcca acctgttcaa gctgtatccg tgggaattta tgttgcgtga
    11101 gatgttttcc accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag
    11161 cattatctcc aacaaggcac ttctaccgct actgtgggag atgttcccga atcacccgaa
    11221 cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa
    11281 accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gaaaacggca aaaccattga
    11341 agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc
    11401 gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc aacccgccgg
    11461 aattggcatt cgtgaagacc gtgcattgat cacccaggat atgtctcggt tttatccaca
    11521 tatttttgtt gagtaatcaa tgatgccgga tggcattcgc catccggtaa ttgttaacct
    11581 atctgcaccg acagcatact caggctgccc atttctatac catcgaccgg aatggtaatt
    11641 ggctcctgcc catcccacgc acctaagaca tacaacaacg gcagatagtg ctccggcgtt
    11701 gggttcgata acgcgccacc ttcatggtcg aggtaattca ccagaggatg ctgctctacc
    11761 gccccttgcc acgtcagatt ctctttcaca tactcattaa acgacatcgc ccaaggatat
    11821 ggtgaactat caccgtgcca cttcactgta cgcaggttat gcaccacgtt gccactggcg
    11881 accaacatta ttccttcatc tcgcagcgct gccagtttgc gccccatttc gaaatgccag
    11941 gcggcaggtt tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac
    12001 atcttaatca gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc
    12061 accgggaccg gtgctaacag ctcaaccaga tgctgtgcca gcacaggcga acccggagca
    12121 ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc
    12181 gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc
    12241 gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct
    12301 tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac
    12361 atgatgatat ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg
    12421 gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag
    12481 gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg
    12541 gcagccacgt ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta
    12601 ctggcgtttt cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg
    12661 cttcctgccg cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc
    12721 ttcggtctgc ttggctattt tggcctggac cgcatgttgc cacatgcgca tccgcaggat
    12781 ttaatgcaaa aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc
    12841 actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc
    12901 agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tattcctgaa
    12961 ggtctggcgg ttgcaggccc ggtttatgca gcaacgggtt ctaaacgcac ggcaattctg
    13021 tgggcgggga tttctggatt ggcagaaatt cttggtggtg tgctggcgtg gttaatcctc
    13081 ggtagcatga tttcccccgt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg
    13141 gtggcgctct cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac
    13201 cccagctatg gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa
    13261 acggcgggaa ttggttaaaa gcaacatcag aagttggcca gcgcatggac aacttctgct
    13321 agcttcacta aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta
    13381 tttgttccgc gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt
    13441 caaatgcagg aaataaccca ccacaacaag agcaatcaat acaatccagt accaggcgat
    13501 aaacatcctt ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat
    13561 atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat
    13621 cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt
    13681 aacatcttgc atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga
    13741 ttaaggcagt aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat
    13801 gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac
    13861 tctggtgcac gcgccatcgt gccatcgtca ttagtcaact cacacagcac accagccggt
    13921 ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt
    13981 acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt
    14041 tttgcgccat cggcaatcgc tgcgcgaacg gttgtaatac gatcagcggc agaaacaccg
    14101 gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg
    14161 ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg
    14221 caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct
    14281 gccgggaaga tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg
    14341 cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag
    14401 gaaagtagcg tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag
    14461 ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag
    14521 aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc
    14581 tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg
    14641 gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg
    14701 gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg
    14761 gtactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa
    14821 atcgctcgcc aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg
    14881 gagaaaaaaa tccgccaaac cctacaagcg cagctgacgc gcctcgacct tgtaagccgt
    14941 gaagagttcg acgtccaaac gcaagtcctg ttacggactc gtgaaaaact ggcgctgctt
    15001 gaacaaagaa tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca gccagatcca
    15061 gagactctcc ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa
    15121 tcttctaatt tacagtgctt tctattacct tatcgcttaa ttccattcag aatatcatca
    15181 caaaaatgaa tgagtgcaat tgaaaatata attgttatgt attttttaag ttttacttaa
    15241 tccatattta gataaactaa tttcataaaa cccgaaaagt aaaatataat atttgaatga
    15301 ttagcaatcc ttaaataaca gttactaatc attcattttc tacttatatt tatataaagg
    15361 aatattatgt ctgcttttaa aaagtcactt cttgttgctg gcgtagcaat gattctttcc
    15421 aataacgtat ttgctgacga aggtcacggc attgttaaat ttaaaggtga agttatctct
    15481 gctccatgtt caattaaacc aggtgacgaa gacttaactg taaatctggg tgaagtagca
    15541 gataccgttc tgaaaagcga ccagaaatct ttggctgagc ctttcactat tcacctgcaa
    15601 gactgcatgt tgagccaggg tggaaccact tattcaaaag cgaaggttac tttcaccaca
    15661 gcgaatacta tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt
    15721 ggtgccacag gcgttggcgt acgtattctg gatagccaga gcggcgaagt gactttaggt
    15781 actccggtag taattacatt caacaatact aacagctatc aggaactgaa cttcaaagct
    15841 cgtatggaat ccccaagcaa agatgctacc ccgggcaacg tttacgctca ggctgactat
    15901 aagattgctt acgagtaatc acattaggcc gggttcgccc ggccacatca ttaacggatt
    15961 aatgataagt ggaacagatg tataaaaaat taaaattaac cacaataagc gaattgatta
    16021 aaaatattta ttgttcatta tccgttctca tcattggttg tgcgtcagct tatgccgttg
    16081 aattcaacaa agatttaatc gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg
    16141 aaactgatgg ccaattaccc gtaggtaaat actcattaaa cgctcttatt aataataaaa
    16201 ggacgccaat ccatctggac ctgcaatggg tattaattga taaccaaact gcagtttgtc
    16261 tgactccaga gcaattaaca ttattaggct ttactgatga gattattgaa gaggctcagc
    16321 aaaacctgat cgatggttgt taccctatcg aaaaagaaaa acaaattaca acttatctcg
    16381 ataaagggaa aatgcaatta tccatatctg cacctcaggc atggttaaaa tacaaagatg
    16441 caaactggac gcctcctgaa ctttgggatc atggtattgc tggggcattt cttgactaca
    16501 atttatatgc ctctcattat gcaccacatc agggcgataa ttcacaaaat ataagttcct
    16561 atgggcaggc tggggttaat cttggggcct ggcgcctgcg tactgattac cagtacgatc
    16621 agtcatttaa caatggcaaa agccaggcga acaacctgga ttttccgcgt atttatttgt
    16681 ttcgcccaat cccagcaatt aatgcaaaac taactatagg tcaatacgat actgaatcct
    16741 ctattttcga ctctttccat ttttctggcg tttcgttgaa aagcgatgag aatatgttgc
    16801 cgcctgatct acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg
    16861 tcactgtctc acagaacaac cgtattattt atcaagaaaa tgttcctcct ggcccatttg
    16921 ctattactaa tttattcaat acattacagg ggcaactgga cgtcaaggtt gaagaagagg
    16981 acgggcaagt cacgcaatgg caagttgcat ctaatagtat tccttatctg acgcgtaaag
    17041 ggcagattcg ctacaccact gctatgggta aaccgaccag cgttggtggt gattccttac
    17101 aacaaccctt cttctggact ggtgaattct catggggttg gctgaacaat gtatccctgt
    17161 atggtggttc agttttaaca aaccgtgatt atcaatctct ggctgccggc gttggcttta
    17221 accttaactc attgggttca ttatcttttg atgtcacacg atctgatgct cagttgcata
    17281 atcaggataa agaaacgggt tatagctacc gcgctaacta ttcaaaacgt tttgaatcta
    17341 ccggtagcca gctcactttc gctggttacc gtttctctga taaaaacttt gtgacaatga
    17401 atgaatatat caatgacact aaccattaca cgaattatca gaatgaaaaa gagagttata
    17461 ttgtcacgtt taaccagtat cttgaatcat taaggttaaa tacatacgta agtttggctc
    17521 gtaatactta ctgggacgcc agcagtaatg tgaattattc attatcactt agccgcgatt
    17581 ttgatatcgg gccattaaaa aacgtctcca cttctctaac atttagccga ataaactggg
    17641 aagaagacaa ccaggatcaa ctgtacctaa atatttcgat tccctgggga accagtagaa
    17701 cattgagcta tggtatgcaa cgaaatcagg ataacaagat ttcgcatact gcttcgtggt
    17761 atgactcttc cgatcgaaat aattcctgga gcgtttctgc ttcaggcgac aatgatgaat
    17821 tcaaagatat gaaagcgtca ctacgcgcca gttatcagca taataccgag aacggtcgtc
    17881 tctatctctc cgggacatca cagcgagaca gttattattc tctgaatgcc agttggaatg
    17941 gttcattcac ggcgactcgt cacggagccg ctttccatga ctatagtggt agtgctgact
    18001 cgcgttttat gatcgacgca gatggtgcag aagatattcc gttgaacaat aaacgcgcgg
    18061 taactaatcg gtatggcatc ggagttattc catcagtcag cagttacata acaacatcat
    18121 taagcgttga tacccgaaat cttccagaaa atgtggatat cgaaaactcg gttatcacca
    18181 ccaccttaac cgaaggtgct attggctacg ccaaacttga tacccgcaag ggctatcaaa
    18241 tcatgggggt tattcgcctg gcagatggta gtcacccacc actggggatt agcgtaaaag
    18301 ataaaaccag ccataaagaa ttaggactgg ttgctgatgg cggctttgta tacctcaacg
    18361 gcattcagga tgacagcaaa cttactttac gctggggtga taaatcctgt tttattcaac
    18421 cacccaatag cagcaactta accaccggaa cggttatttt accgtgtatt agccaaaatt
    18481 aattcaggat gaaactaatg cgctacttaa acacaaagaa tctcattgca gcaggagtat
    18541 tactcgcctg catgagcagc attgcctggg gtgccatcat cccggaccgt acccgtatca
    18601 taatgaatga gtcagataaa ggggaagcat taaaacttac taatcaaagc aaaaatctac
    18661 cctatctggc gcaaacatgg atagaagata cgaaaggcaa taaatcacgt gactttattg
    18721 tcacggttcc ccctatggta cgtttaaatc ccagtgagca aattcaaata agaatgataa
    18781 gtcaggaaaa aattgctcaa ctgcctaatg acagagaaac gctcttctat tttaacgtgc
    18841 gagaaatacc gccaaaaaca gataaaaaaa atgtgatgca agtcactatg cagcacgcat
    18901 tgaaactatt ctggcgacca aaagctattg aattagaaga tgatggggta atgtcttacg
    18961 aaaaagttga aatcatccgt agaaatgatg gttcgatccg ctttaacaat aagatgcctt
    19021 atcacgttac gctgggttat atcggaacaa acggaatcac aatgttgcca caaacgcaaa
    19081 gtttaatggt aaccccttat agccatgcag atacgcagtt taaaaatgtt ccgtcagctt
    19141 ttcaggttgg ttatatcaat gattttggtg gattaagttt ttatgaaata aactgcccga
    19201 cggttaataa ctcttgtaat gtttccgtag ccaaacgaga taaataatat gcgctactta
    19261 ttaattgtaa ttatatttat tataggtttt agtgcattac ctgtatgggc aatggactgc
    19321 tatgctgaac atgaaggggg aaataccgta gtcattggtt acgtaccaag aatttctatc
    19381 cccagcaatg gtaaaaaagg tgataagatc tggcaaagca gtgagtattt tatgaatgtc
    19441 ttctgtaata atgcattacc tgccccatca cctggagaag aatacccatc agcctggact
    19501 aatataatga tgtttttacc aggcggtcag gacttttata atcagaactc ctatattttt
    19561 ggcgtaacat acaatggagt tgattatgat tcgactgccc ctaatgctat cgcagcacca
    19621 gaatgcattg atataaaagg agcgggtacc tttaataatc actataaaaa ccccgctgtc
    19681 tgtagtggag gtcctgagcc tcaattatca gtaacctttc cagctcgcgt acaactctat
    19741 attaaattag caaaaaatgc caatagagtt aataaaaacc tggtgttacc tgatgaatat
    19801 atcgctctag agtttaaagg tatgagtggc gcaggagcta tagaagtaga caaaaatttg
    19861 accttcagga ttcgtggatt aaataatatt catgtccttg actgctttgt taatgtcgca
    19921 ctggaaccag ctgatggcgt tgttgacttt ggtaaaataa attcccgaac aattaaaaat
    19981 accagcgtta gtgagacgtt tagtgtagtc atgacaaaag atcctggtgc ggcctgtact
    20041 gagcaattca atattttagg gagctttttc actacggata ttttgagtga ttatagccat
    20101 ctggatatgg gtaatggact gctattgaag atatttcata aagatggaac agcaacggaa
    20161 tttaaccgct tctcacaatt tgcttctttt tcatcgtcta gtgcaccttc ggtcaccgca
    20221 ccattcaggg cagaacttag tgcgaatccg gcagagactg ttgttgaggg accgtttagt
    20281 aaagacgtca tcctcaaaat cacctataac tagcatctaa tacaaacact aaaacgggcc
    20341 atcaggcccg ttatatcagt gctctaactc cagctctctt gcctgcatca tctgttgaca
    20401 ataccaggca taacgtgcgc aaaaccaggt tctatgttct tcatccatgt taccagtaac
    20461 ggcaagaata tcgaccgggc gaccttctgc gtgcattctg gcaaaactac gcgccaggaa
    20521 atcgaaatta tttaaagaat taagactatg atccataagc acatcctccg gttatcgcat
    20581 accttttaag gccatatgct ttttctgatt tcagtattcc ccaatcagtg tagtgccttt
    20641 aaacataacg tgcgtaaata tattgtcccc gatcacactt tttagtgaaa aataaaaaac
    20701 catattaaaa aactttctct tccagaactt ctctaacatg tgttattatt aacaaagata
    20761 aatgtaagca aggaaactat cccaaagtaa atattgcgtc actaaatgga cattggagtg
    20821 acatatgatt ttattcgccg actataatac cccttatctt ttcgctattt cctttgtact
    20881 gttgatcggc cttttagaaa ttttagcgct tatctgcggt cacatgctct ccggtgctct
    20941 tgatgcacat cttgaccatt atgattccat aaccacaggt catattagcc aggcacttca
    21001 ttatctcaat atcggaagat tgccagctct cgtcgttctc tgcttgctgg ctggtttttt
    21061 tggtcttatt ggcatcctgt tgcagcatgc ctgcatcatg gtctggcaat caccactgtc
    21121 gaacctgttc gtggttcctg tcagtttact gtttacgatt attgcggtgc attataccgg
    21181 taaaatagtt gcgccctgga ttccacgcga ccatagttca gctattacag aagaagaata
    21241 tattggtagc atggcattaa ttaccggtca tcaagccact tcaggtaatc cctgcgaagg
    21301 aaaactcacc gatcaatttg gccaaataca ttatttacta ctggagcctg aagaaggaaa
    21361 atttttcact aaaggagaca aagtgttaat tatttgccga ctttctgcaa cgcgatattt
    21421 ggcggaaaac aatccctggc cacaaatatt gtaatttatt aatcaaaagg aaatttaata
    21481 atggatgata ttgttaattc tgtgccctcc tggatgttta ccgcgattat tgccgtatgc
    21541 attctgttta ttattggaat tattttcgcc aggctctatc gtcgcgcttc ggcagagcaa
    21601 gcttttgttc gtactggttt aggtgggcaa aaagtggtga tgagcggtgg cgcaatcgtg
    21661 atgccgatct ttcatgaaat aatccccatc aatatgaata ctctgaagct ggaagtcagc
    21721 cgctcaacca ttgatagcct gattacgaaa gatcgtatgc gtgtcgatgt agtagtcgct
    21781 ttctttgtac gggtaaaacc gtctgtagaa ggcatcgcca ctgcggcgca gacgttgggg
    21841 caacgcacgc tatcaccaga agacttacgt atgttggttg aagataaatt tgtcgatgcc
    21901 ctccgtgcaa cagctgcaca aatgaccatg catgagttac aggatacccg cgagaacttc
    21961 gtgcagggag tacaaaatac tgtcgcagaa gacctgtcga aaaacggtct tgagctggaa
    22021 agcgtttcac ttaccaactt taaccagacc tcgaaagaac atttcaatcc gaacaatgcc
    22081 tttgacgccg aaggtttaac caaactgact caggagacgg agcgccgtcg tcgcgaacgt
    22141 aacgaagttg aacaggatgt agaagttgcg gtgcgtgaga aaaaccgtga tgcactttcg
    22201 cgcaagttgg agattgaaca acaagaagcg tttatgacgc ttgagcagga gcagcaggtt
    22261 aaaacccgta ctgccgaaca gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt
    22321 gaagcagagc agacgcgaat tctggctgaa cgacaaattc aggaaacgga gatcgagcgc
    22381 gagcaggccg tccgctcaag aaaggttgag gctgaacgtg aagttcgtat taaagagatc
    22441 gaacaacagc aggtcaccga aatcgccaac cagacgaaat cgatcgctat tgccgccaaa
    22501 tcggaacaac agtcccaggc agaagcgcgt gctaaccttg cactcgcaga agcagtaagt
    22561 gcgcaacaaa acgtagaaac cactcgccag accgcagaag ccgatcgtgc taaacaagtt
    22621 gccctaatcg ctgccgcgca ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg
    22681 aaagcagaaa aagaagccgc agagatgcag gcggcggcta tcgttgagtt agccgaagct
    22741 acacgtaaaa agggtctggc ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac
    22801 gtactttctg atgaacaaac cagccttaaa ttcaaactgg ccttgttgca ggcgctacct
    22861 gcggtaatag aaaaatccgt tgagccgatg aaatctattg acggtatcaa gattattcag
    22921 gtcgatggtc tgaatcgtgg cagcgctgcg ggtgatgcaa acacgggtaa tgtggggggc
    22981 ggaaacctgg cggaacaagc attatcagcc gctctctctt accgcacaca ggcaccgctg
    23041 attgactcct tgctcaatga aattggcgtt tcaggcggct cactggcggc attgacttca
    23101 cccttaacct caacaactcc cgtcgccgaa aacgtagaat aaattttcag ctatgaacgt
    23161 ccctggcaca aaaagccggg gattttttat atctgcgttc cgctaaaaac tgcaaatgat
    23221 caggtcgttg caacgttttg cgtgaccgct cggggaaggc aaaactgcct ctgggaaagc
    23281 gttgcgcgag gtccggcgct catcaacaat cggggagtca gcaaagggct gaaacgggaa
    23341 agcccctccc gaggaagggg ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat
    23401 actgattgtg ttgttactcg taagtttcgc agcttattaa cagccaatca gaggggggag
    23461 aaatcctccc tcacccttgt tcctttactt taggttgaaa aaaaatcagc gtcaagaagg
    23521 tttcattaac acatatccct ggcccaaaaa gccggggatt ttttatatct gcgttccgct
    23581 aaaaggtgca aatgctcagg acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa
    23641 ctgcttcagg gaaagcgttg cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa
    23701 ggggctgaaa cgggaaagcc cctcccgagg aaggggcctt atagaaggaa agggttatga
    23761 tgaagttcgt catcatactg gttgtgttgt tactgttaag tttcccgact tactaacaac
    23821 tgatcagagg ggggagaaat cctcccttac ccttgttcct ttactttagg ttgaaaaaac
    23881 aacagcgtca ataggcctgc ctgctacgaa gcgagatctg tgaaccgctt tccggttagc
    23941 cttttttatc ctgttggatc ttcttgatga tgttagtggt cgagcaaccg tcttcgaagt
    24001 tgagcaccag cacttcgccg ccgttggccc agacttcttt actcccggcg atctcttctg
    24061 gtttatagtc gccgcccttc accagtaaat ctggcaggat cccggcgatc aagcgctgcg
    24121 gcgtgtcttc ttcaaacgac accacccagt cgaccgcttc cagtgcgccc agcacaatca
    24181 tacgctgttc gagcgggtta acggggcggg aatccccttt cagccgtttg gtggaggcat
    24241 cgctgttcac agcgacaatc agacggtcac ccagtttgcg agcatttgcc agataggaga
    24301 cgtgcccggc gtgcaggatg tcaaagacac cgttggtcat taccaccttt tcgccgcgtt
    24361 tacgcgctgc cgctacagca agcttcagtt cctcttcggt catcacacca aagccggtat
    24421 ctgcgcgccc acgcactgca ttttccagct cgatcggcga aaccgtggag gttcccagtt
    24481 tgccgaccac cacgccagcc gccgcattgg caaagaagca ggcttcttcc agcgaattac
    24541 ccgctgccag cgttgccgcc aggacgccaa tcaccgtgtc gcccgcaccg gtaacgtcat
    24601 acacttcctg cgcctgggtt ggcatatgca gcggcgcttt acccggttgc agc
//
LOCUS       DAEVZA010000070        24373 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEVZA010000070 DAEVZA010000000
VERSION     DAEVZA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.070"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..668
                     /locus_tag="LAF92_002826"
     CDS             <1..668
                     /locus_tag="LAF92_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBI9791809.1"
                     /translation="VLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGE
                     HVPEIILLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAY
                     EVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRRED
                     YGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLE
                     SLR"
     gene            750..1229
                     /locus_tag="LAF92_002827"
     CDS             750..1229
                     /locus_tag="LAF92_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBI9791810.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1245..1721
                     /locus_tag="LAF92_002828"
     CDS             1245..1721
                     /locus_tag="LAF92_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBI9791811.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1784..2005
                     /locus_tag="LAF92_002829"
     CDS             1784..2005
                     /locus_tag="LAF92_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBI9791812.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2079..2432
                     /gene="cbtA"
                     /locus_tag="LAF92_002830"
                     /pseudo
     CDS             2079..2432
                     /gene="cbtA"
                     /locus_tag="LAF92_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            2537..2910
                     /gene="cbtA"
                     /locus_tag="LAF92_002831"
                     /pseudo
     CDS             2537..2910
                     /gene="cbtA"
                     /locus_tag="LAF92_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            2907..3101
                     /locus_tag="LAF92_002832"
     CDS             2907..3101
                     /locus_tag="LAF92_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791813.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            3114..3227
                     /locus_tag="LAF92_002833"
     CDS             3114..3227
                     /locus_tag="LAF92_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HBI9791814.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(3516..3662)
                     /locus_tag="LAF92_002834"
                     /pseudo
     CDS             complement(3516..3662)
                     /locus_tag="LAF92_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3716..3898
                     /locus_tag="LAF92_002835"
     CDS             3716..3898
                     /locus_tag="LAF92_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBI9791815.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(3999..4328)
                     /locus_tag="LAF92_002836"
     CDS             complement(3999..4328)
                     /locus_tag="LAF92_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBI9791816.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(4500..5558)
                     /locus_tag="LAF92_002837"
     CDS             complement(4500..5558)
                     /locus_tag="LAF92_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI9791817.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(5756..6229)
                     /gene="sbmC"
                     /locus_tag="LAF92_002838"
     CDS             complement(5756..6229)
                     /gene="sbmC"
                     /locus_tag="LAF92_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBI9791818.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(6348..7514)
                     /gene="dacD"
                     /locus_tag="LAF92_002839"
     CDS             complement(6348..7514)
                     /gene="dacD"
                     /locus_tag="LAF92_002839"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBI9791819.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPQIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            7723..9150
                     /gene="sbcB"
                     /locus_tag="LAF92_002840"
     CDS             7723..9150
                     /gene="sbcB"
                     /locus_tag="LAF92_002840"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBI9791820.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(9193..9420)
                     /locus_tag="LAF92_002841"
     CDS             complement(9193..9420)
                     /locus_tag="LAF92_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBI9791821.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(9434..10492)
                     /gene="yeeE"
                     /locus_tag="LAF92_002842"
     CDS             complement(9434..10492)
                     /gene="yeeE"
                     /locus_tag="LAF92_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBI9791822.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(10671..12029)
                     /gene="plaP"
                     /locus_tag="LAF92_002843"
     CDS             complement(10671..12029)
                     /gene="plaP"
                     /locus_tag="LAF92_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBI9791823.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(12019..12081)
                     /gene="yoeI"
                     /locus_tag="LAF92_002844"
     CDS             complement(12019..12081)
                     /gene="yoeI"
                     /locus_tag="LAF92_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBI9791824.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(12296..13225)
                     /locus_tag="LAF92_002845"
     CDS             complement(12296..13225)
                     /locus_tag="LAF92_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9791825.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(13271..14095)
                     /locus_tag="LAF92_002846"
     CDS             complement(13271..14095)
                     /locus_tag="LAF92_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI9791826.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(14178..14432)
                     /gene="yoeB"
                     /locus_tag="LAF92_002847"
     CDS             complement(14178..14432)
                     /gene="yoeB"
                     /locus_tag="LAF92_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBI9791827.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(14429..14680)
                     /gene="yefM"
                     /locus_tag="LAF92_002848"
     CDS             complement(14429..14680)
                     /gene="yefM"
                     /locus_tag="LAF92_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBI9791828.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            14964..15014
                     /gene="hisL"
                     /locus_tag="LAF92_002849"
     CDS             14964..15014
                     /gene="hisL"
                     /locus_tag="LAF92_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBI9791829.1"
                     /translation="MTRVQFKHRHHHHHPD"
     misc_feature    14990..15113
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            15160..16059
                     /gene="hisG"
                     /locus_tag="LAF92_002850"
     CDS             15160..16059
                     /gene="hisG"
                     /locus_tag="LAF92_002850"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBI9791830.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            16065..17369
                     /gene="hisD"
                     /locus_tag="LAF92_002851"
     CDS             16065..17369
                     /gene="hisD"
                     /locus_tag="LAF92_002851"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBI9791831.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAACERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTHPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARGVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSELASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            17366..18436
                     /gene="hisC"
                     /locus_tag="LAF92_002852"
     CDS             17366..18436
                     /gene="hisC"
                     /locus_tag="LAF92_002852"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBI9791832.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFNSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            18436..19503
                     /gene="hisB"
                     /locus_tag="LAF92_002853"
     CDS             18436..19503
                     /gene="hisB"
                     /locus_tag="LAF92_002853"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBI9791833.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            19503..20093
                     /gene="hisH"
                     /locus_tag="LAF92_002854"
     CDS             19503..20093
                     /gene="hisH"
                     /locus_tag="LAF92_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBI9791834.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQSNYG
                     EPFTAAVQKDNFYGVQFHPERSGTAGAKLLKNFLEM"
     gene            20093..20830
                     /gene="hisA"
                     /locus_tag="LAF92_002855"
     CDS             20093..20830
                     /gene="hisA"
                     /locus_tag="LAF92_002855"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBI9791835.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNV"
     gene            20812..21588
                     /gene="hisF"
                     /locus_tag="LAF92_002856"
     CDS             20812..21588
                     /gene="hisF"
                     /locus_tag="LAF92_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBI9791836.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDTETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            21582..22193
                     /locus_tag="LAF92_002857"
     CDS             21582..22193
                     /locus_tag="LAF92_002857"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBI9791837.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTTHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLTTVIENLRKRHQ"
     gene            complement(22290..23270)
                     /gene="wzzB"
                     /locus_tag="LAF92_002858"
     CDS             complement(22290..23270)
                     /gene="wzzB"
                     /locus_tag="LAF92_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBI9791838.1"
                     /translation="MRVENNNVSGQNHDSEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAVNVIYGQAAPKVSDLEETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(23417..>24373)
                     /locus_tag="LAF92_002859"
     CDS             complement(23417..>24373)
                     /locus_tag="LAF92_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBI9791839.1"
                     /translation="LQNADYVIIATPTDYDPKTNCFNTFSVESVIQDAIEINPLAVMV
                     IKSTVPVGFTAAMQTKYHTENIIFSPEFLREGKALYDNLHPSRIVIGERSERAERFAA
                     LLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYAESLGLNTRQIIE
                     GVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAIVDANRTRKDFIA
                     DAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVIIYEPVMKEESFFN
                     SRLERDLVTFKQQADIIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         6221 a         6347 c         6048 g         5757 t
ORIGIN      
        1 ccgtcctgga aaatctgcag cgggaaggct ttcagccgtt cttcgcctgc cagacccgtg
       61 tgcgcgaccc gggccgccgg ggatacacaa aacacatgct gcgtctgcgg cgggccggag
      121 agataaacgg agaacatgtc cctgaaatta ttctgctcaa ctctcatgac ggtacctcca
      181 gctaccagat gctgccgggt tacttcaggt tcgtctgcca gaacgggtgc gtctgtggcc
      241 agtctctggg ggaagtgcgt gttccacacc ggggaaatgt agtggagaaa gttatcgaag
      301 gggcttacga ggtggtgggc gtgtttgacc ggatagagga gaagcgtgat gccatgcagt
      361 cgctggtcct gccgccaccg gcacgccagg cgctggcaca ggcggcactg acttaccgtt
      421 atggtgacga acatcagccc gtcaccaccg ccgacattct gacgccgcga cgccgggagg
      481 attacggtaa ggacctgtgg agcgcatatc agaccatcca ggagaatatg ctgaaaggcg
      541 ggatttccgg tcgcagtgca aaaggaaaac gtatccatac ccgggccatt cacagcattg
      601 ataccgacat taagctcaat cgcgcattgt gggtgatggc agaaacgatg ctggagagcc
      661 tgcgctgatg ccgtttccct gagtgaggga atcattcagc tttcgttcct gaaaaacaca
      721 catttatttg ttatttaagg agttatctca tgaaaaccgt ttctcagaat acccccacaa
      781 tttattcagc tacaacacca gagaataatc cgcctcagtt ggttgccagc ctcgtccctg
      841 atgaacagcg catcagcttc tggccgcagc attttggcct cattccacag tgggtgaccc
      901 tggagccccg tgtcttcggc tggatggacc gtctgtgcga agactactgc ggtggtatct
      961 ggaatctgta caccctgaac aacggcgggg catttatggc acccgaaccg gatgacgatg
     1021 atgacgaaac atgggtactg ttcaatgcca tgaacggtaa ccgcgcagaa atgagcccgg
     1081 aagccgccgg tattgccgcc tgtctgatga cgtacagtca tcatgcctgt cgtacggaga
     1141 attatgccat gacggtccat tattaccggt tgcgggatta cgccctgcag catccggaat
     1201 gcagcgccat tatgcgcatc atcgactgaa tggagagaag cacaatgcaa caactttcct
     1261 ttctgcccgg agagatgacg cccggcgagc gcagcctcat tcaacgggcc ctgaaaaccc
     1321 tggaccgcca tcttcatgaa cccggcgtgg ccttcacctc cacccgtgca gcacgggaat
     1381 ggctgattct gaacatggcg ggactggagc gtgaagaatt ccgggtgctg tatctgaaca
     1441 accagaatca gctgattgcc ggtgaaaccc tcttcaccgg caccatcaac cgcacggaag
     1501 tccatccccg ggaagtgatt aaacgcgccc tgtaccacaa tgccgctgcc gtggtactgg
     1561 cacacaatca cccgtccggt gaagtcacac ccagtaaggc agaccggctt atcaccgaac
     1621 gtctggtaca ggcactgggc ctggtggata tccgggtgcc ggaccatctg atagtcggtg
     1681 gcaaccaggt tttctccttt gccgaacatg gtctgcttta acccgtcaca accacatcac
     1741 acctgttttc acttttatct tctgtcttca gaggtatccc attatgaaaa ttatcacccg
     1801 tggtgaagcc atgcgtattc accaacaaca tccgacatcc cgtctttttc cgttctgtac
     1861 cggtaaatac cgctggcacg gcagtgctga agcgtatacc ggtcgtgaag tgcaggatat
     1921 tcccggtgtg ctggccgtgt ttgctgaacg ccgtaaggac agttttggtc cgtatgtccg
     1981 gctgatgagc gtcaccctga actgagtggg aattctgatg agcagaatta tcgccactac
     2041 cggaccattc ttagccgatt ttctgtaagg attttatcgt gtcagacaca ctccccggga
     2101 caacacttcc cgacgacaat cacgaccgcc cctggtgggg gctgccctgc accgtgacgc
     2161 cctgtttcgg ggcacgtctg gtgcaggagg gtaaccggtt gcattacctt gccgaccgcg
     2221 ccggtatcag aggcctgttc agcgatgcag atgcgtacca cctggaccag gcctttccgc
     2281 tgctgatgaa acaactggaa ctcatgctca ccagcggtga actgaatccc cgccatcagc
     2341 ataccgtcac gctgtatgca aaagggctga cctgcaaagc cgataccctc agcagttgtg
     2401 gttacgttta tctggctgtt tatccgacgc ccgaaatgaa aaaattaact ctccagaata
     2461 gccttctgct acggcctggt gttttcacca cgccactttt ccatttttat atctgcatat
     2521 caggaaaatc ttcagtatga aaacattacc tgtattaccc gggcaggcgg ccagttctcg
     2581 cccgtctcct gttgaaatct ggcagatact gctgtcccga ctgctggacc agcactatgg
     2641 cctcacactg aatgacacac cttttgccga tgaacgtgtg attgagcagc atattgaggc
     2701 aggcatttca ctgtgtgatg cggtgaactt tctcgtggaa aaatacgcgc tggtgctacc
     2761 gaccagccgg gattcagcgc ctgtacccgc tctcagttaa taaacagcat cgatatcctc
     2821 cgggctcgca gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt
     2881 accctgggta agtatccgga ggcgaaatga tgacactgga agccgacagc gttaacgtac
     2941 aggcgctgga tatggggcac attgtcgttg acattgatgg tgttaatatc actgaactga
     3001 ttaataaggc cgctgaaaac ggttattcac tccgcgtggt ggatgaccgt gactctaccg
     3061 aaacaccggc aacttatgcc agccctcacc agttgctgta agacaatgca gtgatgcagg
     3121 gtatagtgcc attaaccaca gaaacagcac tgttgtcctg tattaagcag gcaagagaag
     3181 atgtccttac cctgcgccat ctgcagcttc tgcaccagaa ccggtgaatc tcactcaaca
     3241 ggcttcattt cccctgacgc cagcctgaat acagctggcg ttttcattta taaacagaaa
     3301 ggaaaaccga taataatgga atggacgccc cgaccatgaa gcaacggtag gatattactg
     3361 ataaaccttc cagtggaggt gcgttatggc cgttttaacg attagcattg acctgacaaa
     3421 aaatgaattc caaatccacg gtctgggtag gaacagaaaa atctaagctc agaaaacgga
     3481 ttaaagtaag cgtcaacgga gcaccgtatt gacgcttatt tattggtgag aactacgttc
     3541 catggcagga gttcgtcaac acggttggag ggccattccg gcagtatgct cagaatatgg
     3601 cgcagatacg cttccggatc gataccgttc agacggcagg tgcctgcttt tccggcaaca
     3661 agcaggaatc tcagcgctga agcaggaatt agaggtaaaa acgccctacc gggccatgaa
     3721 tcacccggtc attggagtag taaccaaagc agatttagcc agcatggaac aaatatcttt
     3781 ggtgaaaagt tggttacggg aagctggagc gcacaacgtg ttagtaacca gtgcagttaa
     3841 taacaatgga gttacagaac tttttgcctt gctgcataca gaagaaggct gttgttaatt
     3901 tcgattggtt ctcagatgag aatgatgacg gtgatacaca gataagaatc aatgagcaat
     3961 gagggttgcc gggcaaccct cattgaataa aacgggaatt acttcgcttc gccgtttttc
     4021 atttcgccca tagctttcag ctttttggag atatcgcggc gttctttgga gagctcggca
     4081 tttttgatga tgtaatcgtc aacgcgatct tcatagtcac ctttcatgct ggcgatgatg
     4141 ccctggattg cttcaacgct catccctggc ttgatgtaat cgctcaggtt gtccagcaac
     4201 aggacgcgct tctggttgtc acggatcttt ttctcaacgt cctgaatttc acgttgcagt
     4261 ttgttcttac gacggaacag acgaacaaat tccagtacgt cctggaatga aggcttggta
     4321 gtttccattt ttatacccct gataatgtga gagtcggatt cgtttaagca accgctattc
     4381 gttagggcca acattactca tagctgccgc agatgacaat gcttttatcc cttttcacta
     4441 tcataccctt tatccttgct gaatcgaagc agcagcaaga tgattctgaa gttcaggaat
     4501 tatttgcgca aggcccggca aatcagattc gatagcaact caagctgatg tgccgtttcc
     4561 atgttcagcc acacataacc atagattggt gtttccacaa ccttcaggtc atggtggtta
     4621 tcgagcaact gacgcagctc ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc
     4681 ggctgcggat taccttcgta cagcgcatga acaaggctca gcagtatttg ctgcatcatg
     4741 tgctgggtat cacgaagttt ttgcgcgttc aataacacga aatggctggg gcgcgtggcc
     4801 cagtacgcat tgatttgcaa ctccagcata caaaccagat tgcggttaat ggtctggata
     4861 ccttcatata tcgatttggg aatacgggtt tctttgctgg cgggcgcaat cagcccacgc
     4921 attttcacgg catcggtcag cagtttttgt agatggcttt ccagacgtgg gcgttcgagt
     4981 aagttcggtg agaatgcaga ttgatagacc cgattatact cggtcagact tttcgccagt
     5041 tgaatgcgcc agtggatgaa cgcccgttgt ggccagatac cggtaaacaa cattgccagt
     5101 aaagagccga ggatcacatc gccgcttcgc cataacgccg tatcaatttc acctgtcggg
     5161 gaacccacaa caattgccag cgtcacccca atcaataatc cttgatacgg tttcttgccc
     5221 agcgccagcc aaccgcaaag aaacatcgcc gccgcgcacc agactaacat cagcggtaac
     5281 gaaattaact ccagttgcag agcgataaga cctaaaatcg accccaacac cgtaccgcca
     5341 atacgttcga aggcacgagg gacaacgttc ccccagaacg agattggccc cataatcacc
     5401 accatggtga ccagcggcca ggtgctttcc gggatagtaa acaggcggat aatgagaaaa
     5461 gtgagcagga atgccagcgc gacccgagta ccatgcacaa tgcggtaatg gcggtatacc
     5521 cggatttcaa acgggcttaa tgacttatcg gcacgcactc agaaactccg taatgaagat
     5581 gaaaagcgct cataacacat ctgtcggcca gaaggcgcgc ctctgagtta tccgacggac
     5641 ataccgggaa cagtgtaatt tcccggcagg agcgaaaaat ttgaaaggcg catcttactc
     5701 ttttcgcttt caaaaaaaga gtggtcttcg cgttaacaca ccgccctgag atgaattagt
     5761 gatgttttgg ctgcaccgca acatacattt cgatatccca gtacccatct tccgcgccat
     5821 tgttcaagta aacctcgaag cagggctttg gtaacatttc ataagcactg tcctgcaaga
     5881 ggctattaaa gaactgatac cagggtttag caaaatcatc accgactaca cgagccaccg
     5941 ccaccgcata ttgaccacct gaaatttctg tcagaatgac gccctcactg ttttcgggga
     6001 gcgtaaagtt attcggtacc gtcacgacgg tgtcgcagcg taatttttcg gcgggtgttt
     6061 catccggatt gtcgtaatag acagcaaccc actccttcgg cacaatattt ttgctatcta
     6121 cccacatcat caactgctca aaacctttct ttaccgtctg ttcccacggg ccaacgagat
     6181 ggaaacctgc aacggtacgt ttctcttcct gcttaatctc gtagttcatg acgcctccat
     6241 tgatgctgtt tttatataca gtatagttgc aaaattaaaa ccacaaggaa tgagtgttga
     6301 ttatgcgagc agactcgcac tcctgccagt ctgctgcaaa agaaaggtca ggccttatgg
     6361 tggaaataat cactcaggcg agaaaacatg ctgccttccc cgacagattc cagggtaacc
     6421 agcggccagt gcgccacctg tttatcacgg tcgtaaagtt caatttcccc tacccgctga
     6481 tgggcgctaa ttggcgcggt gagctcttta ccatcaaggg tatatttggc tttgatatgt
     6541 ggaatttcgg ctttcggtag caccatccag aactcttgtt ccgttcccag gtcgatattt
     6601 tctttatcgc cataccagat gcgttccgtt ccgacctttt tcccacggtg caaaatttgc
     6661 accgtagtaa agttttgttg cccccaacgc agtaattttc ttgcctcttc ctcacgacct
     6721 tttgcactgt cagcccccat taccactgca atgagacgac gctgcccatc tacagccgaa
     6781 gcaatgagat taaacccggc accagaagta tgacccgttt tcaggccgtc aacattcatg
     6841 gttttatccc acaacaaccc gttacggttt tgctgggtga taccgttcca ggtgagactt
     6901 ttctcactgt acatatgata aaactcgggc tcgccgtgga tgatagcgcg agaaagcaca
     6961 gctaaatcat aagccgagct atgctggcca ggtgcatcca gaccatgcac tgtttcaaaa
     7021 tgcgtatcct tgagatgcag cttctcggca tagttgttca tcatttcaac aaactgccgt
     7081 tgcccaccgg caatatagtc agccagagca acacaagcgt catttccgga atccacaatt
     7141 aaaccacggc ttaaatcacg taccgatacg cgatcgccct ctttcaaaaa catcagtgaa
     7201 gaaccgacaa acaccggatt atctttcgcc cacgcatcgc gccccacggt gacaatatcg
     7261 tctggcgtaa tgcgatgact atcgatagcg cgatccacga cataacccgt catcagcttt
     7321 gtcaggctgg cgggattgcg ctgttgatgc tcattacccg cggtgaggat ctgaccggtg
     7381 gtgtaatcca tcagtaccca ggacccggca tgaatctgtg gaggctgagg tgaaaaagga
     7441 atgttttccg ccgcaaaacc agacgataag ttaaaaatga acaaagaagc agcaataata
     7501 agacggcgtt tcaacagcaa accctcagga gtttcaaata gctgttcttt ttacggaaat
     7561 acttatgaac tggctggaat aaagtgcaag aaaatgtgac taccctctca tttttatctg
     7621 acatgatctg ttgtcactcg ctgtcaaatt gtcgcgctaa agctgattag cacggtgata
     7681 tttgatactc tggcagacag cagaaataac ggatttaacc taatgatgaa tgacggtaag
     7741 caacaatcta cctttttgtt tcacgattac gaaacctttg gcacgcaccc cgcgttagat
     7801 cgccctgcac agttcgcagc cattcgcacc gatgacgaat tcaatgtcat cggcgaaccc
     7861 gaagtctttt actgcaagcc cgcggatgac tatttacccc agcctggagc agtattaatt
     7921 accggtatta ccccgcagga ggcacgagcg aaaggagaaa acgaagccgc gtttgccgcc
     7981 cgtattcact cgctttttac cgtaccgaag acctgtattc tgggctacaa caatgtgcgt
     8041 ttcgacgacg aagtcacacg caacgttttt tatcgtaatt tctacgatcc ttacgcctgg
     8101 agctggcagc atgataactc gcgctgggat ttactggatg ttatgcgtgc ctgttatgcc
     8161 ctgcgcccgg aaggaataaa ttggcctgaa aatgatgacg gtctaccgag ctttcgcctt
     8221 gagcatttaa ccaaagcgaa tggtattgaa catagcaacg cccacgatgc gatggctgat
     8281 gtgtacgcca ctattgcgat ggcaaaactg gtaaaaacgc gtcagccacg cctgtttgat
     8341 tatctcttta cccatcgtaa taaacacaaa ctaatggcgt tgattgatgt tcctcagatg
     8401 aaacccctgg ttcacgtttc cggaatgttt ggggcatggc gcggcaatac aagctgggtg
     8461 gcaccgctgg cgtggcatcc tgaaaatcgc aatgccgtaa ttatggtgga tttggcagga
     8521 gacatttcgc cattactgga gctggatagc gacacattgc gcgagcgttt atataccgca
     8581 aaagccgatc ttggcgataa cgccgccgtt ccggttaagc tggtacatat caataaatgt
     8641 ccggtactgg cccaggcgaa tacgctacgc ccggaagatg ccgaccgact gggaattaat
     8701 cgtcagcatt gcctcgataa cctaaaaatt ctgcgtgaaa atccgcaagt gcgtgaaaaa
     8761 gtggtagcga tattcgtgga agccgaaccg tttacacctt cagataatgt ggatgcacag
     8821 ctttataacg gctttttcag tgacgccgat cgtgcagcaa tgaaaattgt gctggaaacc
     8881 gagccgcgta atttaccggc actggatatc acttttgttg ataaacgaat tgaaaagctg
     8941 ttgttcaatt atcgggcgcg caacttcccg gggacgctgg attatgccga gcagcaacgc
     9001 tggctggagc accgccgcca ggtcttcacg ccagagtttt tacaaggcta tgcagaagag
     9061 attcagatgc tggcgcagca gtatgccgac gataaagaaa aagtggcgct gttaaaagcg
     9121 ctttggcagt acgcggaaga gattgtctaa tgtgaagcct ccgccgctgg tacggcggag
     9181 gacaattttg agtcaggctt tttgaacggt gatgctccac gctgcatcgc caatctgctg
     9241 ataatcggtg atggcatgtc cttcttctgc cgcccactgt gggattgctt cagttgcctg
     9301 ggtgcaatca aactcaatta ccaactcatc gccgctggcc atttctgcca gtgcagcttt
     9361 tgcttctatg agtggaaacg gacagacctg cgtcaccaca tccagctttt taatcgccat
     9421 ctttacacct tacttaattt gccgcagcag ttgccagtcg tgccttacgc tgcggtcgaa
     9481 catacacaag ccaggacgca gtccagactc cgagaatcat aaatacaagg ccaatccagc
     9541 cctgccaggt catcattgca gtcatgacca gtccattacc gatagagcaa ccacctgcaa
     9601 tgctggcacc gaagcccatc agtacaccac ctaacccgct acgtaatgtt gtttgtgcat
     9661 cagcagcgcg aacgcggaac tcacggctcg ctttggctgc aataaatgac cccacgaaga
     9721 tccctaacac caggaaaacg ccccagttaa tgtatttcac gtcacccgcg accagaaatt
     9781 gcagaatatt ggccgttggg gaagtgattc ctaacccaaa catgcgtccg gttgcttcac
     9841 tcaggggcca ggctaaaagc gcaatcaaac cgatgagtac agcagtgaca aagggatgcc
     9901 agcgtttttc aaacagaata tgagcgatcc cggttcggcg cggcggtaag gtcgcgactt
     9961 tgagttttgg cttcttcaac tctttcatca ccacccagag cgttatcacc agcaaaacgg
    10021 caaccaacgg ccacacagac aaattaaaag tatcagcaat agagttatgt tcagtagtgt
    10081 agtgctgcaa ggtttgattt aaaccactgg cgtgtggaga acgcatcacc gcactcatca
    10141 ccatataagt gaaaagcgcg atccaactgc cgatcaatcc ctcacccgcg cgataccagg
    10201 tcccggtggc acatccaccc gccagaacaa ttcccagccc gaaaagatac ccacctataa
    10261 cagtaccaag ccacgggaac gccccggctt cgtaagtcag taggcccgcc tgaattaacg
    10321 caaaaacccc cacgctttgt accgaaattg caatcagcaa ggcgtaaaac atgcgattat
    10381 ttttcgcgat atacatatcg cgaaaaccac ctgtcaggca aaaacgccca cgctgcatga
    10441 caaatcccag cagagcacca caaattagcc cgcttaatat cattgaaaac ataaattaat
    10501 taaccagatg aatgttaatg gggaaattat tcatgactgg tggaatgcag accaataacc
    10561 aaattctcta aattagaaca aatggttatt aatgaggatt tcgataaaaa agaataaaaa
    10621 aaccggagcc aaatgttcat tcgactccgg tttattatta gaaaagatga ttatgctacg
    10681 tcttcgtact gcggaaccgg attacggaaa ctcttggtta cgcaagccag gtaaatcaga
    10741 ccgatagcag cccagatcag acccagaacc attgagcttt cttccaggtt aacccacagc
    10801 gcaccaacgg tcagcgcacc acacatcggc aggaacagat actggaagtg atctttcagc
    10861 gtcttgttac gcttctcacg gatccagaac tgcgaaataa ccgacaggtt aacgaaggtg
    10921 aacgccacca gcgcaccaaa gttgatcagc gccgtagcca ttaccaggtc gaagttgatt
    10981 gccagcaggg caatcgcgcc aaccaggatg atgttcatcg ccggagtacg ccatttcggg
    11041 tgtacataac cgaagaaact tttcgggaac acgccgtcac gacccattac gtacatcaga
    11101 cgcgctacgc ctgcgtgcgc cgccataccg gacgccagta cggtaatggt ggagaagatc
    11161 agcgcaccga cctggaacgc tttacccgca acgtacagca tgatttcagg ctgtgacgca
    11221 tccggatctt taaagcgaga gatatccggg aagtacagct gcaggaagta agttgcaaag
    11281 atgaagatca tgccgccgat cagcgcggtc aggaaaatcg cacgcgggat cacgcgctct
    11341 gcatctttgg tttcttccga cagattgctg atgccgtcaa agccggtaaa ggagaagcac
    11401 aggattgtcg ccccggtaat catcgggata acatgcgcat cgccagacca gaatggacga
    11461 gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc cataaacaac catgcccaga
    11521 atcaccgcga tcagcactac ctgcaacacg acgattacag tgttgaagtt cgctacggat
    11581 ttcagactac gcaggttaaa ggcggtcata aaggccacca gcgccaccac aaacatccac
    11641 gatgggatgg aaggcaccag agcttcaaaa tagattttcg ccagcagaat gttgatcatc
    11701 ggcgcgaaca gatagtcgag cagagaagac caacccacca taaagccgac agtcgggcta
    11761 atggatttct gggcgtaagt atatgcagag ccagcagaag gatagcggcg aaccagcttc
    11821 ccgtagctca gagccgtaaa caggatcgca atcaatgcga acgcataggc tgtcggcaca
    11881 tgaccatccg tgaggcctga aacgatacca aaagtatcaa acagcgtcat cggctgcata
    11941 taggcaagac ccatcattac aaccggaact aacgtaagcg ttttacgcaa ttccacgcga
    12001 gaggtgtttg gagtaacgtt atgcgacatg gtcattctcc tttacggtga taaccgtcgc
    12061 gtaagcaaaa aattgcccca tttttttgga ttcctcagcg acaacaactg tcgattttta
    12121 gtaaatatct atccggtacg aagcccggcc tcttggtatg aatgattggt ttgaagcaaa
    12181 aaataaccga cgctgatgaa acgtcggttt ttagtcattt tttgacagcg gcgcattgtg
    12241 cctaaatggg ggggaaatga caagagaatg agaggcttgt cataataatt tttctttaaa
    12301 tggctgattt tccgtcatca gattttgcat aaacaccggc gatcgcacta tttgctaaaa
    12361 tttcatcccg ccaccagccg ctggcaagtc ctgcggtctg ttcattccag ccaatccaga
    12421 cgggttcaaa gagagtttgg gcgaccactt tcttctcgat taacgcgcca ctatcgagaa
    12481 aacgttgcgc cagataacgg ggtaaatagc cgcagccgag gccgctaatt tgcagctcca
    12541 gcttggtttt aaaatcgaaa acggtaatgg cttcttgttc gtcgagaagt tgtgaggccg
    12601 tagaagcgcc tgcctgcgca gtgtccccca ccacaatcgc ccgatatcgt ttaatgatac
    12661 gacggtttaa tggttcttct tcctgggcta acggatgatg aggcgcgacc gcaaagactt
    12721 gttccagatc gcccaaccgc gagaaaccaa actcactgga agatggtggc tcatgcatcg
    12781 cgccaacaat aatatccgct cgcccctgag tcagggcatc ccatgaacca ccgagtacgc
    12841 cattgataaa cttcaggcgc gtcacgctat gatgttgata aaaagcttca atgagtggcg
    12901 caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac cagctcgttt tcccagcctt
    12961 catgcagttt gatcgcctgc ttttccagtt ctcgcacggt atgcagaact tcccgcccct
    13021 tctctaataa catttttccg gtacgggtga atttagcgcg gtgaccgcta cgatcaagca
    13081 attgaatatt aaggtcgctt tccagcttgt gaacggtgta actcagagcg gacggcgtct
    13141 tgtagagttt agccgacgcc gccgcaaaac tgccttcttt ttctaaggca tcgaggatca
    13201 tcaacacatc cagcagtggt ttcatacacg cccccttgca gtgtatgcgg cgtcctgctg
    13261 gcgtgatcgt ttactccagc ggcattacca gcggatcggg atactggtat tcaaatccca
    13321 gttcattaca aatccgactg ccatcaataa tcttaccttt gccgctgtcc agactatttc
    13381 tgaattgcgg aggttccagc cccagtaaac gggccatctg cggatagaaa acattacgcg
    13441 cagggtgagc tggcgcacat atattataga tgtgtccgcc tttaggtgcc tgtaacaaca
    13501 gagtgatagc gccaataaca tcttctaaat ggactaaatt aacaccatgt tcaccatcag
    13561 gcgcggtttt tccggcaaag aagcgtccgg gatgacgccc cggtccgacc aggcccgcaa
    13621 gacgcagaat atcgaccgaa gtaccgggta aattgtgcag ccagtcttcg agttcttcta
    13681 atactcgtcc actgttggtt accggattac gcggtgtagt ttctttcacc gtgccttgcg
    13741 cgtcgccata gacagacgtt gagctggtaa aaataatgcg gggaatacga tgggccagcg
    13801 cgctatccac taactcttgt accgcttgta aatagaactc atcgccgggg ccgctacgac
    13861 gtgccggaag cgtaatgacc agcgcatcgg catccatcag ggcatccaga tcgtcagaat
    13921 cgcaaactaa ctcaggctcc atgcgaagca gatagctatc aatgccactc attcgggccg
    13981 cttcgacacc atcttgtgtg gttttactcc cggtgacttg ccagcctcgc gctgaaagtg
    14041 acatcgccag cggcatgcct aaccacccta acccgacaat tgcgaccttt ttcatccgtt
    14101 atctcctgat acatcttaga tctataaggc tacgctagcg tatcaaaact gacaattcat
    14161 tctatgaatg aatctcttca ataatgataa cgacatgctg caatgagcag tgaatcgtcg
    14221 gtaaccgcgt ataccagacg gtgctcctct gtaatgcgtc gggaccagaa acctgacaaa
    14281 ttatgtttca ggggttctgg cttcccctta ccttcaaatg gtgttctgcg ggtatctttg
    14341 ataagttcat tgatcttttt aacaattcgc ttatctgttt cctgccagta cagataatca
    14401 tcccatgact cctcagacca gattagtttc actcaataat gtccttttcc gttcctttgc
    14461 ctgatttcag gctatcgatt gagtccatca atctccgggc gttagcgggg gagcgcagta
    14521 gataagccgt ctcttccagc gagttgtatt cttcgagtga catcagaaca caagcctctc
    14581 cattctgacg agtaataagg atcggggcat gatcttcaac ggctttcatc attgttgccg
    14641 acaaattctg gcgcgcttcg ctgtagctaa ttgtacgcat ttcaatctcc tcttttgtac
    14701 agttcattgt acaatgatag gcgttaatta actatttatt aattagtctg tagatcaaga
    14761 cattgtcagt gagacgaaaa atccaggttt cgctattttg ggtgccgtca ggttagggga
    14821 cactcctctt agccccctcc tttccccgct cattcattaa acaaatccat tgccataaaa
    14881 tatataaaaa agcccttgct ttctaacgtg aaagtggttt aggttaaaag acatcagttg
    14941 aataaacatt cacagagact tttatgacac gcgttcaatt taaacaccgc catcatcacc
    15001 atcatcctga ctagtctttc aggcgatgtg tgctggaaga cattcagatc ttccagtggt
    15061 gcatgaacgc atgagaaagc ccccggaaga tcatcttccg ggggcttttt tattgcgcgg
    15121 ttgataacgg ttcagacagg tttaaagagg aataagaaaa tgacagacaa cactcgttta
    15181 cgcatagcta tgcagaaatc cggccgttta agtgatgact cacgcgaatt gctggcgcgc
    15241 tgtggcatta aaattaatct tcacacccag cgcctgatcg cgatggcaga aaacatgccg
    15301 attgatattc tgcgcgtgcg tgacgacgac attcccggtc tggtgatgga cggcgtggta
    15361 gaccttggga ttatcggcga aaacgtgctg gaagaagagc tgcttaaccg ccgagcccag
    15421 ggtgaagatc cgcgctactt taccctgcgt cgtctggatt tcggcggctg ccgcctttcg
    15481 ctggcaacgc cggttgatga agcctgggat ggcccactct ccttaaacgg taaacgtatc
    15541 gccacctctt atcctcacct gctcaagcgt tatctcgacc agaaaggcat ctcttttaaa
    15601 tcctgcttac tgaacggttc tgtcgaagtc gccccgcgtg ccggactggc agatgcgatt
    15661 tgtgatctgg tttccaccgg tgccacgctg gaagctaacg gcctgcgcga agtcgaagtt
    15721 atctaccgct cgaaagcctg cctgatccag cgcgatggcg aaatggaaga atctaaacag
    15781 caactgatcg acaaactgct gacccgtatt cagggtgtta tccaggcgcg cgaatcaaaa
    15841 tacatcatga tgcacgcacc gaccgaacgc ctggatgaag tcatcgccct gctgccaggt
    15901 gccgaacgcc caaccattct gccgctggcg ggtgaccaac aacgcgtagc gatgcacatg
    15961 gtcagtagcg aaaccctgtt ctgggaaacg atggaaaaac tgaaagcgct gggtgccagt
    16021 tcaatcctgg tcctgccgat tgagaagatg atggagtgat cgccatgagc tttaacacaa
    16081 tcattgactg gaatagctgt actgcagagc aacaacgcca gctgttaatg cgcccggcga
    16141 tctccgcttc tgaaagcatt acccgcactg ttaacgatat tctcgataac gtgaaagcac
    16201 gcggtgatga cgccctgcgg gaatacagcg cgaagtttga taaaaccacg gttaccgcgc
    16261 tgaaggtgtc tgctgaggaa attgccgccg cctgcgaacg cctgagcgac gagctaaaac
    16321 aggcgatggc ggtggcagta aagaatattg aaaccttcca cactgcgcaa aaactgccgc
    16381 cagtagatgt agaaacgcag ccaggcgtac gttgccagca agtcacgcac ccggtggctt
    16441 cagtgggttt gtatattcct ggcggctccg ccccgctctt ctcaacggta ttaatgctgg
    16501 cgactccggc gcgtatagcg ggctgtaaaa aagtggtgct gtgctcaccg ccgccgattg
    16561 ccgatgaaat tctttacgcg gcgcagctgt gcggcgtgca ggacgtgttt aacgtcggcg
    16621 gcgcacaggc cattgccgca ctggcgtttg gtacggaatc tgtgccaaaa gtggacaaaa
    16681 tcttcgggcc aggcaacgcc tttgtcaccg aagcaaaacg ccaggtaagc cagcgtctgg
    16741 acggtgcggc gatcgatatg cccgcaggcc cgtcggaagt gctggtcatt gccgacagcg
    16801 gtgctacgcc tgatttcgtg gcttctgatt tgctctccca ggctgaacac ggcccggact
    16861 ctcaggtgat tttactgaca cccgctgctg atatggcgcg tggcgtagcc gaagctgtcg
    16921 aacgccagtt ggcagagctg ccgcgtgccg aaaccgcccg tcaggcactg agcgccagcc
    16981 gtctgatcgt gactaaagat ttagcgcagt gcgtagagat ctccaaccag tacggcccgg
    17041 agcacctgat catccagacc cgcaacgccc gcgatctggt cgatggcatc accagcgccg
    17101 gttcggtatt tcttggtgac tggtcacccg aatcggcggg tgattacgcc tccggcacca
    17161 accacgtact gccgacttac ggttacaccg ccacctgttc cagcctcggg ctggcagatt
    17221 tccagaagcg tatgaccgtg caggaactgt cgaaagaagg tttctccgag ctggcttcga
    17281 ccatagaaac actggccgcc gccgagcgcc tgaccgccca taaaaatgcc gttactttgc
    17341 gtgttaacgc ccttaaggag caagcatgag caccgtgact attaccgatt tagcgcgtga
    17401 aaacgtccgc aacctgacgc cgtatcagtc ggcgcgtcgt ctgggcggta acggcgacgt
    17461 ctggctgaac gccaacgaat accccacagc cgtggagttt cagcttactc agcaaacgct
    17521 caaccgctac ccggaatgtc agccgaaagc ggtgattgaa aattacgcgc agtatgcagg
    17581 cgtaaaaccg gagcaggtgc tggtcagccg tggcgcggac gaaggtattg aactactgat
    17641 tcgcgctttt tgcgaaccag gtaaagacgc catcctctac tgcccgccaa cgtacggcat
    17701 gtacagcgtc agcgctgaaa ccattggcgt cgagtgccgc acagtgccga cgctggacaa
    17761 ctggcaactg gacttgcagg gcatttccga caagctggac ggcgtaaaag tggtctatgt
    17821 ttgcagcccc aacaacccga ccgggcaact gatcaatccg caggattttc gcaccctgct
    17881 ggagttaacc cgcggtaagg cgattgtagt tgccgatgaa gcctatatag agttttgccc
    17941 acaggcatcg ctggctggct ggctgacgga atatccgcac ctggctattt tgcgcacact
    18001 gtcgaaagct tttgctctgg cgggccttcg ttgtgggttt acgctggcaa acgaagaagt
    18061 catcaacctg ttgatgaaag tgatcgcccc ctacccgctc tcgacgccag ttgccgacat
    18121 tgcggcccag gcgttaagcc cgcaggggat cgtcgccatg cgcgaacggg tagcgcaaat
    18181 tattgcagaa cgcgaatacc tgattgccgc actgaaagag atcccctgcg tggagcaggt
    18241 ttttaactct gaaaccaact acattctggc gcgctttaaa gcctccagcg cagtgtttaa
    18301 atctttgtgg gatcagggca ttatcttacg tgatcagaat aaacaaccct ctttaagcgg
    18361 ctgcctgcga attaccgtcg gaacccgtga agaaagccag cgcgtcattg acgccttacg
    18421 tgcggagcaa gtttaatgag tcagaagtat ctttttatcg atcgcgatgg aaccctgatt
    18481 agcgaaccgc cgagtgattt tcaggtggac cgttttgaca aactcgcctt tgaaccgggc
    18541 gtgatcccgg agctgctgaa gctgcaaaaa gcgggctaca agctggtgat gatcaccaat
    18601 caagatggtc ttggaacaca aagtttcccg caggcggatt ttgatggccc gcacaacctg
    18661 atgatgcaga tcttcacctc gcaaggcgtg cagtttgatg aagtgctgat ttgtccgcac
    18721 ctgcccgccg atgagtgcga ctgccgtaag ccgaaagtaa aactggtgga gcgttatctc
    18781 gctgagcaag cgatggatcg tgccaatagt tatgtgattg gcgatcgcgc gaccgatatt
    18841 cagctggcgg aaaacatggg cattaatggt ttacgctatg accgcgaaac cctgaactgg
    18901 ccaatgattg gcgagcagct cactaaacga gaccgttacg cccatgtagt gcgcaacacc
    18961 aaagagacgc aaattgacgt ccaggtgtgg ctggatcgcg aaggtggcag caagattaac
    19021 accggcgttg gcttctttga tcacatgctg gatcagatcg ccacccacgg cggtttccgc
    19081 atggaaatca acgtcaaagg cgacctctat atcgacgatc accacaccgt cgaagatacc
    19141 ggcctggcgc tgggcgaagc gttaaaaatt gccctcggcg ataaacgcgg tatttgtcgc
    19201 tttggttttg tgctaccgat ggacgaatgc cttgcccgct gtgcactgga tatctctggt
    19261 cgcccgcacc tggaatataa ggccgaattt acctaccagc gcgtgggcga tctcagcacc
    19321 gaaatgatcg agcacttctt ccgttcgctc tcatacacca tgggcgtgac cctgcacctg
    19381 aaaaccaaag gtaaaaacga tcaccaccgt gtagagagcc tgttcaaagc ttttggtcgc
    19441 accctgcgcc aggccatccg cgtggaaggc gataccctgc cctcgtcgaa aggagtgctg
    19501 taatgaacgt ggtgatcctt gatactggct gcgccaacct gaactcggtg aagtctgcca
    19561 ttgcgcgcca cggttatgaa cccaaagtca gccgtgaccc ggacgtcgtg ttgctggccg
    19621 ataaactatt tttacccggc gttggcactg cacaagcagc gatggatcag gtgcgtgagc
    19681 gcgagctgtt tgatctcatc aaagcctgta cccaaccggt gctgggcatc tgcttaggga
    19741 tgcaactgct tgggcggcgc agcgaagaga gcaacggcgt cgacttgctg ggcatcattg
    19801 acgaagacgt gccgaaaatg accgactttg gtctgccgct gccgcatatg ggctggaacc
    19861 gcgtttaccc gcaggcaggc aaccgcctgt ttcaggggat tgaagatggc gcgtactttt
    19921 actttgttca cagctacgca atgccagtca atccgtggac catcgcccag agtaattacg
    19981 gcgaaccgtt cactgcggcg gtacaaaaag ataacttcta cggcgtgcag ttccacccgg
    20041 agcgttctgg taccgctggc gctaagttgc tgaaaaactt cctggagatg tgatgattat
    20101 tccggcatta gatttaattg acggcaccgt ggtgcgtctc catcagggcg attatggcaa
    20161 acaacgcgat tacggtaacg acccgctgcc acgcttacag gattacgctg cgcaaggtgc
    20221 cgaagtgttg catctggtgg atctgaccgg ggcaaaagat ccggcaaaac gtcaaatccc
    20281 gctgattaaa accctggtcg cgggcgttaa cgttccagtg caggttggcg gcggtgtgcg
    20341 tagcgaagaa gacgtggcgg cgttactgga agcgggcgtt gcgcgcgtgg tggttggctc
    20401 caccgcggtg aaatcaccag aaagggtgaa aggctggttt gaacgcttcg gtgccgatgc
    20461 cttagtgctg gcgctggatg tccgtattga cgagcaaggc aacaagcagg tggcggtcag
    20521 cggctggcaa gagaattcgg gcgtttcact ggaacaactg gtggaaacct atctgcccgt
    20581 cggcctgaaa catgtgctgt gtaccgatat ctcgcgcgac ggcacgctgg caggctctaa
    20641 cgtctcttta tatgaagaag tgtgcgccag atacccgcag gtggcatttc agtcctccgg
    20701 cggtattggc gacattaacg atgttgctgc cctgcgtggc actggcgtgc gcggcgtaat
    20761 agttggtcgt gcattactgg aaggtaaatt caccgtgaag gaggccatcg catgctggca
    20821 aaacgtataa ttccgtgtct cgacgttcgc gatggtcagg tggtgaaagg cgtacagttt
    20881 cgcaaccatg aaatcattgg cgatattgta ccgctggcaa aacgctacgc tgaggaaggt
    20941 gcagacgaac tggtgttcta cgatatcacc gcttccagcg atggccgtgt ggtagataaa
    21001 agctgggtat ctcgcgtggc ggaagtaatc gacattccgt tttgtgtggc gggtgggatt
    21061 aagtctctgg aagatgccgc gaaaattctt tcctttggcg cggataaaat ttccatcaac
    21121 tcgcctgcgc tggcggatcc gacgttaatt actcgcctgg cggatcgttt tggcgtgcag
    21181 tgtattgtgg tgggtattga tacctggtac gacactgaaa ccggtaaata tcatgtgaat
    21241 caatataccg gcgatgaaag ccgcacccgc gtcactcagt gggaaacgct cgactgggta
    21301 caggaagtgc aaaaacgcgg tgccggagaa atcgtcctca acatgatgaa tcaggacggc
    21361 gtgcgtaacg gttacgacct cgaacaactg aaaaaagtgc gtgaagtttg ccacgtcccg
    21421 ctaattgcct ccggtggcgc gggcaccatg gaacacttcc tcgaagcttt ccgcgatgcc
    21481 gacgttgacg gtgcgctggc agcttccgta ttccacaaac aaataatcaa tattggtgaa
    21541 ttaaaagcgt acctggcaac acagggcgtg gagatcagga tatgttaaca gaacaacaac
    21601 gtcgcgaact ggactgggaa aaaactgacg gacttatgcc ggtgattgtg caacacgcgg
    21661 tatccggtga agtgttaatg ctgggctata tgaacccgga agccttagat aaaaccatcg
    21721 aaagcggcaa agtcaccttc ttctcgcgca ctaaacagcg actgtggacc aaaggcgaaa
    21781 cgtcgggcaa tttcctcaac gtagtaaata ttacgccgga ctgcgacaac gacacgttac
    21841 tgttgctggc gaatcccatc ggcccgacct gccacaaagg caccagcagc tgcttcggcg
    21901 acaccactca ccagtggctg ttcctttatc aactggaaca actgctcgcc gaacgcaaat
    21961 ctgccgaccc ggaaacttct tacaccgcca aactgtatgc cagcggcacc aaacgcattg
    22021 cgcagaaagt gggcgaagaa ggcgtggaaa ccgcactggc cgcgacggta catgaccgct
    22081 ttgagctgac caacgaggca tctgatttga tgtatcacct gttggtgctg ttgcaggatc
    22141 aggggctgga tttaacgacg gtgattgaga acctgcgtaa acggcatcag tgagttgcgg
    22201 gataagcgga tgcgatgttg tcgtcgcatc cggcaaaaaa aacgggcaag gtgccaccac
    22261 cctgcccatt ttctttaaaa ccgaaaagat tacttcgcgt tgtaattacg cagagcatta
    22321 cgccccaaca caatccccgc gccaaccatg ccgcccagca acaccgccag aatcaaggtg
    22381 attgcctttt tcgggctatc gcgacgaata ggtaacgtcg gtttcattac ataacggtaa
    22441 gcatgaatat caagatcatc aacttttaag ctttcgatat ccagcaggtt ttgacgagtc
    22501 tgatagtagt ttggtgagaa caccaacgga cgggtcgcct catgcttaat catcgactcc
    22561 agcgcttcgc tccccaaaag gaacaacgta tcttgcgtga tatcttcgcc agtctgttga
    22621 atttgcggtt ttgtcacctg cgcctgattc gcatactgca acgcttcctg aatctgacgg
    22681 atacgcagat ctttctgctc ctgcgcaacc acttcctgcg ttcttaaaga gtcctgcaag
    22741 tttttccgtc ccagagcaat gttgtctttg agatcctttt ctagctcttg attcacttta
    22801 tcatcaactt gctgaatgta ttgggccaac ttcatttgtg ctccctctgc agtttgccca
    22861 acataagaaa cagtcaatgg taattgctgg ttcttaacag aaggttcgat ggtaagtttt
    22921 tctggttctt cctgattatc cagcgtttct gctaatgcag agaaggcaga actgaagcga
    22981 ccaattaacg tctcctctaa atccgatact ttcggtgcag cctgaccata gataacattc
    23041 acggcattgt tatagccagc aatttgtccc acgtcgggct gagtgataat tgctgttgac
    23101 gtccatttct ccttcgctac tgccaaatat ccaatagcca gggcaatagc cacaatgacg
    23161 gaaatgatga ttgtcatctt gccacgccac aactgcacta gtaaatcaat caaatcaatc
    23221 tgttccgagt catggttttg cccagaaaca ttattatttt ctactctcat cattacccta
    23281 acggacgaaa taattttaag tcgccatagt ttagcgataa tcgccatgaa tgctatagga
    23341 taaatgataa aaataatgaa ttattacaaa gggaacataa gagaatcagg gcgaaaatcg
    23401 ccctgataac aggatgttag tcactgccaa agagatcgcg ggtgtagact ttatcagcaa
    23461 catctttaag ctcttctgcc atacggttgg agataatgat atcggcttgt tgtttgaagg
    23521 tgacgagatc gcgctccaga cgcgagttga aaaatgactc ttccttcatt actggttcat
    23581 agatgattac ttctacgcct ttcgccttga tacgcttcat aattccctga atagaagacg
    23641 cacggaagtt atctgaacca ctcttcataa tcaggcgata gatccccacc acttgcggct
    23701 tacgtgacaa aatggcatcc gcaataaagt ctttgcgcgt acggttagca tcgacaattg
    23761 ccgagatcag gttattcggc acagactggt agttcgccag tagctgctta gtatctttcg
    23821 gcaggcagta gccaccatag ccaaatgacg gattgttgta atggttgcca atacgcgggt
    23881 caaggcaaac accttcgata atctgacgcg tattgagacc taaactttct gcatagctat
    23941 ccagttcgtt aaagtacgct acgcgcattg ccagataggt attggcaaac aatttaatcg
    24001 cttctgcttc ggttgagtcg gtaaacaggg tcgggatatt ttgcttgatg gccccttcct
    24061 gtaatagcgc agcaaaacgc tctgcacgtt cagaacgctc accaataaca atgcgggatg
    24121 gatgaaggtt atcgtaaagg gctttgcctt cgcggaggaa ttctggggaa aaaataatgt
    24181 tctcagtatg atatttcgtc tgcattgccg cggtaaaacc aaccgggaca gtggacttga
    24241 taaccataac tgcaagtgga tttatttcaa tggcatcctg aatgacagat tccacgctaa
    24301 acgtattaaa acagttcgtt tttggatcgt aatctgtagg cgttgcgatg atcacgtaat
    24361 ctgcattctg tag
//
LOCUS       DAEVZA010000071        24047 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEVZA010000071 DAEVZA010000000
VERSION     DAEVZA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.071"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..192
                     /gene="glpC"
                     /locus_tag="LAF92_002860"
     CDS             <1..192
                     /gene="glpC"
                     /locus_tag="LAF92_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="anaerobic; with GlpAB catalyzes the conversion of
                     glycerol-3-phosphate to dihydroxyacetone phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBI9791840.1"
                     /translation="GTYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIE
                     MSTSLRCEHPITLLAQALA"
     gene            386..1288
                     /locus_tag="LAF92_002861"
     CDS             386..1288
                     /locus_tag="LAF92_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBI9791841.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSPNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(1329..1799)
                     /locus_tag="LAF92_002862"
     CDS             complement(1329..1799)
                     /locus_tag="LAF92_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBI9791842.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1854..3056)
                     /locus_tag="LAF92_002863"
     CDS             complement(1854..3056)
                     /locus_tag="LAF92_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBI9791843.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            complement(3156..3698)
                     /locus_tag="LAF92_002864"
     CDS             complement(3156..3698)
                     /locus_tag="LAF92_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791844.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            3977..4402
                     /gene="nudI"
                     /locus_tag="LAF92_002865"
     CDS             3977..4402
                     /gene="nudI"
                     /locus_tag="LAF92_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBI9791845.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(4441..5043)
                     /locus_tag="LAF92_002866"
     CDS             complement(4441..5043)
                     /locus_tag="LAF92_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBI9791846.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            5351..6490
                     /gene="arnB"
                     /locus_tag="LAF92_002867"
     CDS             5351..6490
                     /gene="arnB"
                     /locus_tag="LAF92_002867"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBI9791847.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            6494..7462
                     /gene="arnC"
                     /locus_tag="LAF92_002868"
     CDS             6494..7462
                     /gene="arnC"
                     /locus_tag="LAF92_002868"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBI9791848.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            7462..9444
                     /gene="arnA"
                     /locus_tag="LAF92_002869"
     CDS             7462..9444
                     /gene="arnA"
                     /locus_tag="LAF92_002869"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBI9791849.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            9441..10331
                     /locus_tag="LAF92_002870"
     CDS             9441..10331
                     /locus_tag="LAF92_002870"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBI9791850.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSSNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            10331..11983
                     /gene="arnT"
                     /locus_tag="LAF92_002871"
     CDS             10331..11983
                     /gene="arnT"
                     /locus_tag="LAF92_002871"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBI9791851.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            11980..12315
                     /gene="arnE"
                     /locus_tag="LAF92_002872"
     CDS             11980..12315
                     /gene="arnE"
                     /locus_tag="LAF92_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBI9791852.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            12315..12701
                     /gene="arnF"
                     /locus_tag="LAF92_002873"
     CDS             12315..12701
                     /gene="arnF"
                     /locus_tag="LAF92_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBI9791853.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(12695..12961)
                     /gene="pmrD"
                     /locus_tag="LAF92_002874"
     CDS             complement(12695..12961)
                     /gene="pmrD"
                     /locus_tag="LAF92_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBI9791854.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVARKTQ"
     gene            complement(13071..14426)
                     /gene="menE"
                     /locus_tag="LAF92_002875"
     CDS             complement(13071..14426)
                     /gene="menE"
                     /locus_tag="LAF92_002875"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBI9791855.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDESATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(14423..15385)
                     /gene="menC"
                     /locus_tag="LAF92_002876"
     CDS             complement(14423..15385)
                     /gene="menC"
                     /locus_tag="LAF92_002876"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBI9791856.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFVRETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(15385..16242)
                     /gene="menB"
                     /locus_tag="LAF92_002877"
     CDS             complement(15385..16242)
                     /gene="menB"
                     /locus_tag="LAF92_002877"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBI9791857.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(16257..17015)
                     /gene="menH"
                     /locus_tag="LAF92_002878"
     CDS             complement(16257..17015)
                     /gene="menH"
                     /locus_tag="LAF92_002878"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBI9791858.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(17012..18682)
                     /gene="menD"
                     /locus_tag="LAF92_002879"
     CDS             complement(17012..18682)
                     /gene="menD"
                     /locus_tag="LAF92_002879"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBI9791859.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(18771..20066)
                     /gene="menF"
                     /locus_tag="LAF92_002880"
     CDS             complement(18771..20066)
                     /gene="menF"
                     /locus_tag="LAF92_002880"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBI9791860.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(20145..20450)
                     /gene="elaB"
                     /locus_tag="LAF92_002881"
     CDS             complement(20145..20450)
                     /gene="elaB"
                     /locus_tag="LAF92_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBI9791861.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(20505..20966)
                     /locus_tag="LAF92_002882"
     CDS             complement(20505..20966)
                     /locus_tag="LAF92_002882"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI9791862.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            21031..21948
                     /gene="rbn"
                     /locus_tag="LAF92_002883"
     CDS             21031..21948
                     /gene="rbn"
                     /locus_tag="LAF92_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBI9791863.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            22136..23356
                     /gene="elaD"
                     /locus_tag="LAF92_002884"
     CDS             22136..23356
                     /gene="elaD"
                     /locus_tag="LAF92_002884"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HBI9791864.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLMRVI
                     NSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRQVNRNCYLDKYGDANINDYYRDLEIK
                     HSQPQNRASGKRVS"
     gene            23768..24028
                     /locus_tag="LAF92_002885"
     CDS             23768..24028
                     /locus_tag="LAF92_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI9791865.1"
                     /translation="MISDFTPSDYFYQPWVTALSPTPRHVSPEQLEKTVRYLTQTVHP
                     RNADNIDNLNRSVEYIKEFFVSSGARVTAQGVPDWLLRLCAW"
BASE COUNT         5954 a         6331 c         5859 g         5903 t
ORIGIN      
        1 ggtacttacg gtttcaaaaa agagaactac cccacctcac aagccatcgg tgcgccactg
       61 ttccgccaga tagaagagag cggcgcggat ctggtcatca ccgactgcga aacctgcaaa
      121 tggcagattg agatgtccac gagccttcgc tgcgaacatc cgattacgct actggcccag
      181 gcgctggctt aaccgacctt ttgatgcccg gtaagcacat tgcttatcgg gcatttttgc
      241 gagcgcgatt ccacgcacca tgtatgcgtt gcaacgcttt gaaaattcct ctgaaaatgt
      301 ctcgcaaagg ctgaaactga cagatgtcaa aggcccggga tagacgtaat gtcgcgtcat
      361 cataaatatc aggtgacgga caaccatgac cgaatcaaca acatcctccc cgcatgatgc
      421 ggtgtttaaa acctttatgt tcacacccga aaccgcacgg gattttctcg aaatacattt
      481 accagaacca ctgcgcaagc tttgcaacct gcaaacctta cgcctggaac ccactagttt
      541 tattgaaaaa agtttacgcg cttactactc ggatgttttg tggtccgtgg aaaccagcga
      601 cggtgacggc tatatctact gcgtgattga acatcaaagc tctgcagaaa agaatatggc
      661 ttttcggcta atgcgctatg ccactgccgc catgcagcgt cacctggata aaggctatga
      721 cagagttccg ctggtggtgc cattgctgtt ttatcatggc gaaacatcgc cctacccgta
      781 ctcacctaac tggctggatg agtttgacga tccgcaactt gcccggcagt tgtacaccga
      841 agcttttccg ttggtggata tcaccatcgt acctgacgat gagatcatgc aacatcggcg
      901 tatagctctg ctggaactga ttcaaaagca tattcgcgac cgcgatttaa tcggcatggt
      961 cgacaggatc accacgcttt tggttagagg cttcactaat gacagccagc tacaaacact
     1021 gtttaattat ctgctgcaat gcggcgatac ctcccgtttc acccgtttta ttgaggagat
     1081 tgccgaacgt tcaccactac aaaaggagag attaatgact attgctgaac ggctacggca
     1141 ggaagggcat caaattggct ggcaggaagg tatgcatgaa caagccatta aaattgcttt
     1201 gcgcatgctg gaacagggca ttgatcgtga ccaggtgctc gcggccaccc agctaagcga
     1261 agccgatctg gcagcgaata accactaatt aacacaggcc cacagccgat ccccatgggc
     1321 ctttgatatc aataactacc ttttatgcgt ggcccatttt tgcctgattt aaacatcgcc
     1381 agtcgttgat ccagggcatc gctgtagagc atcgtgtcaa cgccaacagc gacaaagttc
     1441 gctccccacg ccaggcattg ctgcgccata tcaggagcca cagccagaaa accagccgct
     1501 ttacccgcag cacggatccg ccgaatgctg gtttcaataa ttcgctgcac ttccgggtgc
     1561 ccggcgttat ccgggtagcc caacgacgca gaaagatccg ctggtccaat aaacacgcca
     1621 tcaatccctt cgacgtcgag gatctcgtcc aggttatcca gtgccgtttt actttccacc
     1681 tgcactaaca gacaaagcga atcgttgact tgcgccatgt aattctcaat gcgtccccag
     1741 cgcgcagccc gtgccacact ggccccaaca ccacgctcac cgtagggagg gttggacaag
     1801 ttttgccgga gggggaatgt gccggtgcag taaaccggca cagagtggcg gaatcagaca
     1861 gataaggtca tggactcaat aacctcaatc cagccatgct cactggcgat atcctggccg
     1921 tttaaccaac ggcgcagcat attcagtgcc atcattgcgc acacttcctg acggatagcc
     1981 aggctgtagc gcgtagtgct gaaacgcaca cgcagagcga aagtgccgtc tggtgtggct
     2041 agcgcaaagt tgagatgttc gttctcgaaa cccgaaacag ccagtgccag cccggcaaaa
     2101 tggttggccc gccgttctgt aatccagtgc gcagtttgcg ccagggtttc ttcctgtgaa
     2161 ggaaccactt cacacgccag caatggagca cctgcgcgag aaagttgcaa agccaataaa
     2221 ccgccggtga attgctcgct caacgtcagg ctgaactggc ggttttgcaa ctcgcgactg
     2281 atctgcgcgg gcagtccttc ggtgccttcg aaaatcacgc tctgtccggc aacacgttta
     2341 acgtccagcc acagtttttc catcgcctgt tgctcgcttg ccggtccggt gagtttcagt
     2401 tcgatgatcg gcattgagga gcgatagccc attgttacgc ccggcggcag ttgtagagtg
     2461 tccaggcttt gtgccagatc gctttccgaa cgaccaaagg tagttaaacg cagacaaacc
     2521 ggcggctgtg gtaaagaaaa acgctcgcgc aggcgcggca ggatttcgtg ctcgaccatc
     2581 accttaaatt ctgacggtac gccgggagta aagaacatca ggcaacgatt aagctgcacg
     2641 gcaaaaccac aggcggtgcc taccgggtta ttgataaatt cagcactggc aggcagctcc
     2701 gcttgtttac ggttgctcgg tgccattact cgtccacgtt cgtggaaata gcgttccatc
     2761 tctttgagcc aggcttcatg cagcaccatg ccttcacctt ttgctgtcgc agcggcgagt
     2821 gcgcttaaat catcgctggt cggtcccagc ccgccgttaa cgatcagcac atcggcgtgc
     2881 tgactacgtt cgcgaagaat ggtgactaag tcatcaaggt tatcccccac cgtattgcgg
     2941 cgagataatg gcagcccctg atgaaagaaa aaatcggcca gccaggcagc gttagtgtca
     3001 acgatttgcc cgtgtaacac ttcatccccg gtggatagca tttccacttt taacatggtt
     3061 atctcctgct tttgttatcg aatcactata acgcaaaggg agataaggcg tagtaagaaa
     3121 ctgaatggcg cgacagaatg ccgcgccgga agggatcaga aactggcgtt aaccccaaca
     3181 tacgggccgt cagccacggc gttgtcgcgg ttaccgtctt tacccgacag attcaggtag
     3241 cgataacccg cctcaatact gactggacgc ataatggtgt aacgcgcgcc agcattcgct
     3301 tcctcataac tttgaatacc gctggagagc gaatccggag agtagtaata ctcgccaaac
     3361 aaacggaagc tgttgccaat tttccactgc aaaccacctc ctaccgctgc ggcataacct
     3421 tcatcgccgt aattcgggtt ggtgtaaacg ccttttccgc caacggtcgc cattaacggc
     3481 ccgagaggca gattcaaccc cagccccacg cccgcgacgt caccgtcgtc gtcgttatgt
     3541 gtccagttac cgcttaaagc caggcccgtc gattcagtcc caaaaccgac accaatattg
     3601 gtgtattctt tacccgcctg gccgctgatg ctcattgcat tgaccgatgc agaaaccaac
     3661 agcattccgg ccagacctgc tagcgcaatt tttttcatta tcccctttcc cacaagcaat
     3721 taatcaaaat tcccaaagct gcgggattgt actgcctggc acagaggatt caatgagcga
     3781 aaaaagggat ctagtcactt attgtaacta aatacttctt aaataattaa acgccgaaaa
     3841 aataaacaca ctttaccgat acacacaaaa ataataataa gtgcagttat cattgcaaaa
     3901 gagattactg tcactttcac atatttctga gttaccgtat tctcatggtt tctttttatt
     3961 tataaggaat acccctatgc gacaacggac tattgtatgc cctttgattc aaaatgatgg
     4021 tgcttatttg ctgtgtaaaa tggccgacga tcgcggcgtt ttccccggtc aatgggcgct
     4081 ttcgggtggc ggcgtggagt ctggcgaacg aattgaagag gcactacgcc gcgaaattcg
     4141 cgaagaactg ggagaacagc tgcttttgac agaaatcacg ccgtggacct tcagcgatga
     4201 tattcgcacc aagacgtatg cagatggtcg caaggaagag atttatatga tttacctgat
     4261 ttttgactgc gtttctgcca accgagaagt gaaaataaac gaagagtttc aggactacgc
     4321 gtgggtaaaa cctgaagatc tggtgcatta tgatttaaat gttgccaccc gaaaaacgtt
     4381 acgtttgaaa ggtcttcttt aacaatgatg acagccatca ctggctgtca gattgaaaat
     4441 ttagtggtta acgaattccc catccagata aactttgcct ttttccacat gcatgactaa
     4501 accatccaga taatcaggtt taaatgtcgc atcacgctta tttttagcaa tatatgtcag
     4561 gcaatgatta tgggtgaaaa taacgatatt tttatcaggc gctttgcttt gtaagtcctt
     4621 aattgcacta taaatctcat taccgcactg aagaagtcgt ttatctaccg tcagcttttt
     4681 acccgcggaa aaccaggtcg ccgactgaat ggtccggacg gtattactgg aataaagatc
     4741 gaaatcaggg atgtcagcgc taaaagcgtt gcccagttca cgggcatcct gggtaccttt
     4801 aaccgtaata cctgttttat ctgacaagca ttgattggtt gaacgatcgc aacgttcagc
     4861 atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt ctggccaatg ttttattgtc
     4921 gatacgtggc aaaccattgc tactccaggc ggcatgagta cccagtccgg caattgcagc
     4981 gagcgccagt aaaatgatga aatatttttt tgacttcaat gatgagcggc aaaaagctaa
     5041 cacgtaaact ccacctatag acaagcgcaa ccagacaatt accgtaaaat tgagcgacat
     5101 ttctggcgat aattagcagt tggtgtaata ttaaaaatcc tacgatgtcg gcaaaatgcc
     5161 tcaaaatttt gccaaatgca aagcctaaat aagaaaaaat ataaaaattt caatatttac
     5221 gtctaatatt agtttcttaa ggttaagtta atattctatc cttaaaattt cgctccaaat
     5281 ggcaaaatat acacaacact ctttatagca aatataagtg gacaggtatt caatggcgga
     5341 aggaaaagca atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg tggaggaact
     5401 cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca accggtccga agaatcaggc
     5461 gcttgagcaa gccttttgcc agttgacggg aaatcagcat gccatcgcgg tcagttcagc
     5521 caccgccgga atgcatatca cgctaatggc gttggaaatt ggcaagggcg atgaagtgat
     5581 tacgccttcc ctgacctggg tttcaaccct caatatgatt tccttgttgg gtgcaacgcc
     5641 ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg cctgaagcga tcgagtcagc
     5701 cataacgcca cgcactaaag ccatcattcc ggtgcattat gccggtgcgc cagcagatat
     5761 tgacgccatt cgcgccattg gcgaacgtta cggcatcgca gttatcgaag atgctgccca
     5821 tgccgtcggt acgtattaca aagggcgaca tattggcgca aaaggtaccg ctattttttc
     5881 atttcatgcc attaaaaata ttacctgtgc ggaaggtggc ctgattgtaa ctgataatga
     5941 aaaccttgcc cgccagctac ggatgctgaa atttcacggt ctgggtgtcg atgcctatga
     6001 cagacaaacc tggggccgtg caccgcaggc tgaagtctta acaccgggct ataagtacaa
     6061 tctgaccgat attaacgccg cgattgccct gacacagtta gcaaaattag agcacctcaa
     6121 cacccgtcgg cgcgaaattg cccagcaata tcagcaagca ctggcagctc tcccctttca
     6181 gccattaagc cttcccgcct ggccgcacgt tcacgcctgg catctgttta ttattcgtgt
     6241 cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa aagaaagagg
     6301 cattggtacc gggttacatt tccgcgccgc tcatacacaa aaatattatc gcgagcgttt
     6361 tcccacgctg tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt ctttgccgct
     6421 gttcccggat atgactaccg ccgatgccga ccgtgtcatc acagcccttc agcaactcgc
     6481 aggacaataa gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt
     6541 ttataacgag caggaaagct taccggaatt aatcaggcgc accaccacag cctgtgaatc
     6601 gttggggaaa gagtatgaga tcctgctgat tgatgacggc agtagcgata attccgcgca
     6661 tatgctggtc gaagcctcac aagcggagaa cagccatatt gtgtctattt tgcttaaccg
     6721 caattacggg caacattcag cgattatggc gggattcagt cacgttaccg gcgacttaat
     6781 tattaccctt gatgccgatc tccagaatcc gccagaagaa atcccccgcc tggtggcaaa
     6841 agccgatgaa ggttacgacg tggtagggac tgtacgccag aaccgccagg acagctggtt
     6901 tcgtaaaaca gcttcgaaga tgattaaccg gcttattcag cgcaccaccg gcaaagcgat
     6961 gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca
     7021 ctgccatgaa cgtagcacct ttatcccgat tctggcgaat atcttcgccc gccgtgccat
     7081 tgaaattcca gtacatcatt ccgagcgtga gtttggtgaa tccaaataca gttttatgcg
     7141 cctgattaat ttgatgtacg acctggtgac ctgccttacc acaacgccgc tacgtatgct
     7201 gagtctgctc ggcagcatta ttgcgattgg aggttttagc attgcggtgc tgctggtgat
     7261 tttacgcctg accttcggac cacaatgggc ggcagaaggc gtctttatgc tatttgccgt
     7321 gctgtttact tttattggcg ctcagtttat cggcatggga ttactcggtg aatatatcgg
     7381 caggatctac accgatgtcc gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc
     7441 atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga tatgggatgc
     7501 ctcggtattg aagctctgct ggctgccggt tacgaaatta gcgccatttt tacccatacc
     7561 gataatcccg gtgaaaaagc cttttatggt tcggtggctc gtctggcagc ggaaagaggc
     7621 attccggttt atgcgccgga tgacgttaat catccgctgt gggtggaacg cattgcccaa
     7681 ctgtcgccag atgtgatttt ctctttttat tatcgccatc ttatttgcga cgaaattttg
     7741 cagctcgctc ccgcaggtgc atttaatctg catggttcgc tgttaccaaa atatcgtggt
     7801 cgcgcgccgc tgaactgggt gctggtcaac ggtgaaacgg aaactggcgt tacattgcac
     7861 cgaatggtga aacgtgccga tgccggggcc attgtggcgc aactgcgcat tgccattgcg
     7921 ccagacgata tcgctattac gctgcatcat aaattgtgcc atgccgcgcg ccagctactg
     7981 gaacagacat tacccgccat taaacacggt aatattctgg aaatcgccca gcgcgaaaac
     8041 gaagccacct gttttggtcg cagaacgccg gatgacagtt tccttgaatg gcataaaccg
     8101 gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg tgccttcagc
     8161 tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa
     8221 gcacagccgg ggagcgtgat ttctgttgcg ccactgctga ttgcctgtgg cgatggcgcg
     8281 ctggaaatcg tcaccggaca ggcgggcgac ggcattacta tgcagggctc gcaattagcg
     8341 cagacgctgg gcctggtgca aggttcacgc ttgaatagcc agcctgcctg caccgcccga
     8401 cgccgtaccc gggtactcat cctcggggtg aatggcttta ttggcaacca tctgacagaa
     8461 cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg atattggcag tgatgcgata
     8521 agccgttttc tgaatcatcc gcattttcac tttgttgaag gcgatatcag tattcattcc
     8581 gaatggattg agtatcacgt caaaaaatgt gatgtcgtct tgccgctggt ggcgatagcc
     8641 acgccgattg aatatacccg caacccgctg cgcgtatttg aactcgattt tgaagagaat
     8701 ctgcgcatta tccgctactg cgtgaagtac cgtaagcgaa tcatcttccc gtcaacttca
     8761 gaagtttatg ggatgtgtag cgataaatac ttcgatgagg accattctaa tttaatcgtc
     8821 ggcccggtga ataaaccacg ctggatttat tcggtatcaa aacaattact tgatcgggtg
     8881 atctgggcct atggcgaaaa agagggttta cagttcaccc tcttccgccc gtttaactgg
     8941 atgggaccgc gactggataa ccttaatgcg gcacgaatcg gcagctcccg cgctattacg
     9001 caactcattc tcaatctggt agaaggttca ccgattaagc tgattgatgg cggaaaacaa
     9061 aaacgctgct ttactgatat tcgcgatggt atcgaggcgt tataccgcat tatcgaaaat
     9121 gcgggaaatc gctgcgacgg tgaaattatc aacattggca atcctgagaa cgaagcgagc
     9181 attgaggaac tgggcgagat gctgctggcg agcttcgaaa aacatccgct gcgccatcat
     9241 ttcccaccgt ttgcgggctt tcgcgttgtc gaaagtagca gctactacgg caaaggatat
     9301 caggacgtag agcatcgtaa accgagcatc cgcaatgccc accgctgcct ggactgggag
     9361 ccgaaaattg atatgcagga aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt
     9421 gatcttacgg ataaaccatc atgaccaaag taggcttacg cattgatgtc gatacctttc
     9481 gtggcacccg tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat aatattcagg
     9541 ccagcatttt tttcagcgtc ggcccggaca atatgggccg ccatctctgg cgactggtga
     9601 agccacagtt tttgtggaag atgctgcgct caaatgcggc atcgctttat ggctgggata
     9661 ttttactggc aggtacggcc tggccaggta aagagattgg tcatgccaat gccgatatca
     9721 ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc ctgggatcac catgcctggc
     9781 aagcccgtag cagtaactgg gatcggcaaa caatgatcga cgatattgca cgcggtcttc
     9841 gcactctgga agagattatc ggtcaaccgg taacctgttc tgccgctgcg ggctggcgtg
     9901 ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac agcgattgtc
     9961 gtggggccat gccgttccgt ccattgctcg aatcaggaaa ccctggcact gcgcaaattc
    10021 cggtgacctt acccacctgg gatgaagtga ttggtcggga tgtgaaagca gaagatttta
    10081 acggttggtt actcaaccgc atcctgcgag ataaaggcac gccggtttat accattcatg
    10141 cagaagttga aggctgcgct tatcagcata attttgtgga tctcctcaaa cgcgcagctc
    10201 aggaaggcgt gacattttgc cctttaagcg aactgttatc agagacgttg ccgctcggac
    10261 aagttgttcg cggaaatatt gccggacgtg aaggctggct gggttgccaa caaattgcgg
    10321 gtagtcgctg atgaaatcgg tacgttacct tatcggcctc ttcgcgttta ttgcctgcta
    10381 ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc gatgaaacgc gttatgcgga
    10441 aatcagtcgg gaaatgctgg catccggcga ctggattgtg ccccatctgt tagggctacg
    10501 ttatttcgaa aaacccattg ccggatactg gattaacagc attgggcaat ggctatttgg
    10561 cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg accctgttaa ctgccgcgct
    10621 ggtgacctgg tttactctgc gcttatggcg cgataaacgt ctggctctac tcgccacagt
    10681 aatttatctc tcattgttta ttgtctatgc catcggcact tatgccgtgc tcgatccgtt
    10741 tattgcattc tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc aggcacagac
    10801 gtggaaaggc aaaagcgcag gatttttact gctgggaatc acctgcggca tgggggtgat
    10861 gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat gggtagcgac
    10921 gcaaaaacgc tggaaagatc tctttattta cggctggctg gcggttatca gttgcgtact
    10981 gacggttctc ccctggggac tggcgatagc gcagcgggag cctgacttct ggcactattt
    11041 tttctgggtt gagcatattc aacgctttgc actggatgat gcccaacata gagctccgtt
    11101 ctggtactac gtgccggtca tcattgccgg tagcctgccg tggctgggat tactccccgg
    11161 tgcactgtac acaggctgga aaaaccgcaa gcattccgca accgtctatt tgttgagctg
    11221 gacgataatg ccgctgctgt ttttctccgt cgctaaaggt aaattgccca cctatattct
    11281 ttcctgcttt gcatctctgg caatgctgat ggcgcattac gctttgctgg cagcaaaaaa
    11341 taatcctctg gcgctgcgga ttaatggctg gattaacatc gcttttggcg tcactggcat
    11401 tattgccaca tttgtggtct ccccgtgggg accaatgaac acgccggtgt ggcaaacctt
    11461 cgagagctat aaagtctttt gtgcctggtc gattttttcg ctatgggcat ttttcggctg
    11521 gtacacctta acaaacgtcg aaaagacctg gccttttgcc gcgctttgcc cgctggggct
    11581 ggcgttgctg gtaggatttt caattcctga cagagttatg gaaggaaaac atccgcaatt
    11641 ttttgtcgag atgacacaag aatcacttca gccaagccgc tatattctta ctgatagcgt
    11701 cggtgttgcc gcaggtctgg catggagcct gcaacgcgat gacatcatca tgtatcgcca
    11761 gacaggtgag ttgaaatacg gccttaatta tccggatgcg aaagggagat ttgtcagcgg
    11821 tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag gggattatta ctctcgtgct
    11881 ttcggttgac cgcgatgaag atatcaacag tctcgccatt ccgcccgcag atgccatcga
    11941 tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa tgatctggct aacattagtc
    12001 tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac ctgctttgtg
    12061 gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg gactggcgct ggcttgtctt
    12121 ggtcttgcca tggtgctctg gctgctggtc ttgcagaacg taccggtagg cattgcctac
    12181 ccgatgttaa gtctgaattt tgtctgggtg acgctggctg cagtaaaact gtggcacgaa
    12241 ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta ttggtggcat tgtgatcctc
    12301 gggagtacgg tgtaatgggc ctgatgtggg gattattcag cgtcattatt gcctcagttg
    12361 cgcaattaag cctgggtttt gcggcgagtc atctgccgcc gatgacgcac ttatgggatt
    12421 ttatcgcggc tctgttagct tttggcctgg atgcccggat actgttactc gggctgctgg
    12481 gttatctgct ttccgttttt tgttggtata aaacgctaca taaacttgcg ctaagcaaag
    12541 cctatgcctt gctcagtatg agttatgtgc tggtatggat tgcttcaatg gtacttcccg
    12601 gctgggaagg gactttttcg ctgaaagcac tactgggagt agcctgtatt atgagcgggt
    12661 tgatgctgat tttcctgccc atgacaaaac aacgttactg agttttccgt gccactttac
    12721 actgccgttc ccactcatcg gggctataac tgcttgccga gaggactttc accgtatgca
    12781 gcttttcacg attaatacaa taaagcgcat tctgcaaagg cgagagtaaa tcccccactt
    12841 tcacggcgaa gtcactcttc acttcggcga tcatttttat cgcgccgcca gcatcgcaga
    12901 gcataagtac atgtctgtta tcttgtttat tgcagcacga ttttttgacc agccattcca
    12961 ttgcattatc ctgtttgcta agagttttca catcaattta cagattaaca gataaactct
    13021 ttatcaacgg caatggaatg gcagaaatga gaagtgaaac ggagggttaa ttattgctga
    13081 cgttgcaccc actcttttag cgcctgacgt gaaattttaa taccgccgtt tttcagttca
    13141 ggcggcaacg ttaaccaacg caccggttgc tgaaaacggg ccagcttatc tttcacccat
    13201 tcactaagat caacgctctc gtggtcatac tccatcaccg ccaccggtcg atgaccaaac
    13261 tccttgtcgg caacggggac gataaacacc tgcaaaaccg caggatgtgc agcaattacg
    13321 cgctcgactt cttccggctg aataccctct ccgccactga agaatagatt gtctaaacgt
    13381 ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat cgcgcgtagc gtaccagcct
    13441 tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt aaccttctgc catactggca
    13501 gcccgcagcc acacttcatt attaacgatt ttcacttccc gacccggcag cggcgaacca
    13561 acgtctgcca ggccgtcggc ttctttcgca cacaccgtgg aggcaaactc ggtcagacca
    13621 tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt ccgtcaactc gaccgggata
    13681 gccgcgccgc caagtaacac cgctttcagg gaaacggaac tgcggttaac cagcaaacgc
    13741 cagagttgtg ttggcaccag tgaagcgtga gtacagcctg ccagcatttg ctccaatggc
    13801 tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc agcgccacat aattccctga
    13861 ccggagacgt gaaacagcgg taaagagagc aaccagtcat cgtgatcgcc aaacggaatc
    13921 aacgataaca ccccttcggc actggcaaga tgggcctgat aagtatggac agcggctttc
    13981 ggcaacccgg tagaacccga ggtcaacgtc attgagcaca gacgcgtcgg ctgccacgta
    14041 gcggcatgtg cgccttcaac ccgctgaatg tgcagcgacg ttaacgccgg aaacgtgttt
    14101 tccccatccg gcaccagcgc aaattgcagc gtcagattgg gcagcaattc ttcaagcaac
    14161 ggttgcggca gctgagggtt cacgggcaac acccgcgccc cgcattgcag taacgccagc
    14221 caggcgagca gcgtttgcgg cgtattccac gcccgcaaca tcacgccgct gccctcaaca
    14281 acgccctgcg ccgcaaagcc cgtagctgac tcatcgacgc gagcacaaag ctcgcgccag
    14341 ttaagttgct cgtcattaag acgtaaggcg atggcttctc cccgcacttg ccgccagtga
    14401 cgccacggcc agtcagagaa gatcataaca accgctccag tgcatcaact tccacgacag
    14461 gcagcgtgct acccggccag cgacgtacct gctgcgcctg catcagatcc agcgtgtcca
    14521 gccctggaat ggtgtccggc gttaaccagg cggcaatccg cgccagttgc gttaagccta
    14581 agctcgattc aatggaagaa ctgatcaccg ccgtcagccc cagcgcgtgc gccgcctgta
    14641 cctgctcgcg tactttctcc agactgcccg tgagcgtggg tttgataact accgcgcgca
    14701 cgccctcttc agccacaaag gcaaaatccg gctcgcgcaa gctttcatcc caggcaatgg
    14761 caatgccggt ttcacggaca aacgctcgcg aatcatcgcg ggttttgcac ggctcttcga
    14821 gaaacgcgat gcggtggcga taatccgggt taacatattt ggcaaactgc tggcctttca
    14881 gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag atccggaatt gcctccagca
    14941 acagattaac caccatgccg tcgcgcacgg cttcgtacaa tcccactttg accttcgcca
    15001 ctttctcgcc tggcatatca gcaagtttga ggatcagatc gtcaggatcg ccattacaca
    15061 gtggtgccgc acggtaattg gccgcctgcg ggagcgtatc tgccagctcc gccaatgcac
    15121 agcttacgcc aaaggcaacg gaaggcatct gcggtagctc gcaatcgcct gccagccagt
    15181 tatttaccca ggcaagcagc acgctttgcg cctcttccca ggtttcctgg ctgaagcccg
    15241 gcagtgggga gatctccccc cacccttcgc gctcgccctc acgcaggcaa acgtacagcc
    15301 cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc cgcgtccatg gggatctgcc
    15361 agcggtatac ctgcgcgcta cgcattacgg attccgttta aatttgctga aatcaggctg
    15421 acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct tccgtcatgt agaacagcat
    15481 ggtggcgttg cccgccagct cctgcagccc cgcctgcccg tcacagtcgg cgttcagcgc
    15541 agctttcagg cagcgcagcg ccatcgggct gttttgcagc atttcgcggc accaacggac
    15601 ggtttctttt tccagatccg ccagcggtac cacggtgttg acaaggccca tatccagcgc
    15661 ctgttttgcg tcgtactgac ggcacaggaa ccagatttct cgcgcttttt tctgcccgac
    15721 gatacgagcc atgtaggaag cgccccagcc gccgtcgaag gaaccgactt tcgggccagt
    15781 ctgaccgaag atggcattat ctgctgcgat agtcaggtcg cacatcatgt gcagaacgtg
    15841 accaccgccg atggagtagc cagccaccat cgcgacaacc ggtttcggac aggtacggat
    15901 ctgacgctgg aagtccagca cgttcaagtg atgtacgccg gaatcatctt tatagccgcc
    15961 gtaatcacca cgcactttct ggtcaccacc ggagcagaac gctttatcgc ctgcgccagt
    16021 cagaatgatc acgccgatat tgtcgtcata acgcgcatct gccagtgcct ggatcatctc
    16081 ttttaccgtc agaggacgga aggcattgcg cacctgcgga cgattaatgg tgatttttgc
    16141 gataccgtcg gtggattttt cataacgaat gtcctcgaaa ccttcggagc agtcgtgcca
    16201 ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc atattgtgtc ctttagtcag
    16261 aaacgcaaga tctgcgccag acttgcgatt acgccagcgg gattttcccg atgcgcgtta
    16321 tgtccggcgc gaggaatgac atggcagtcg gcagccagtt ccgccgccag ggcgcggaat
    16381 ttgctgtcac gttcaccaca taaataataa aacgcaaatg tgcgggcact aaggttagca
    16441 cgtaaatcag gctggacggc gagagaagtc gcctccagca tggcggcaag ggttgcgcca
    16501 ttattgttgc tgcgcagcgc caccagctcc cggcgttgat cgtcattgag cgaggcaaaa
    16561 acaggctgtt gataccagtc ggcaaatacc gccgttaacg gttctgtgca aaaacgctgc
    16621 gcccattggc gatcggaacg ctgacgttcc gcacgttgtt cagcattttg cagccccgga
    16681 tgcccgcctt cgacaataac cccacaaagc cccgccagcc cctggcaagc cgccatcatc
    16741 gccacccgtc cgccaagcga gtaccccacc agccagaagt caaggatgtt gtaactaacc
    16801 aaggttttac gcagtaagtc ggtgacatca tcaaatccat cgacgctaat cgccgccgaa
    16861 ccaccatgac ctgggagatc aacatacaac cgtgagtagt cggcaaacgc ctcgcccact
    16921 tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa acaccagcca gggtaaacct
    16981 ggttttccgt gttttgcctg cgcgtgcagg atcataaatg gcttacctgc gccagaagtt
    17041 gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat ttcaatcacc gtggtggttg
    17101 gcgtgcgcca ggcgtcggca aatgccgttt caagttcctg ccagttttgc ggacgatgat
    17161 atttcagctc gaacatcgcg gcggcgtgct caaaatggac gttttgcggc atcagataga
    17221 aacgctcacg ctcgctttgc ggcgttggca acagcgagaa aatttgcccg ccgttgttgt
    17281 tcaccacaat taataccagc ggcgcagaaa cctgacgcaa taacgccagc gcgttgagat
    17341 cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt gcccgctgaa
    17401 cgcctgcggc ggtcgaaagc agtccgtcga taccgctggc ccctcggttg ctgtacaccg
    17461 ggtaacctgc cggaagttgc gaaagcgcat caatcagacg taccaccagg ctgttaccaa
    17521 caaacaattg cccctgttca ggcagatagt cgcagatgcg atgcgccagt tgcgcttcgc
    17581 caaacgcatc acggcgggca ataaccgcct gcattgcctg ttccgccagg cgcgggattt
    17641 caacgcacca gggctggcgt ttttctgccg gatgcagctc cagccagtcg gcaatattgg
    17701 caattaagcg acgtccgcga tggtgtgccg gatcaagtcg cccttcaatg tcatcaacaa
    17761 tccagtactc ttctggttca cagcttgcct gccattgcag gagccgtttc cccgtcaggc
    17821 tgcttcccag ttgcaccaca atttgcgcct gctgcagctc gctggtcgct ttggcattgc
    17881 ctaaccaaag atcggcacac ggcagcggct gcccggtttg tgacagcaca tcgccaatca
    17941 gcggccagcc aagagtttgc gcccacaggg caactttttt gccctcttcc gcactcatgc
    18001 gcccggcaac caccacgccg cgcttttgtc gccagaagaa ccagtcgcgc tgtttttcac
    18061 tttccagacg aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag tcacccagac
    18121 gctgttgcca gctaagcccg gtatcgtcca tttcgccata cagcggttca gcaaacgggc
    18181 agttgatatg gactcccccc gcatgaagcg taccgagagc gtggtcgatg gtagaaacca
    18241 gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga aatactgtgc gtggggtgag
    18301 aggcgaacat tcccggctgg cgaattgcct gattcgcgcc gcagtcaatt agctccggcg
    18361 ggcgatcggc ggttaacagg atcagttttt ctccggttaa cccagcttca atcagtgccg
    18421 gatagagatt tgctaccgcc gtgccggagg tcacaatcac cgccaccggc tgcttgctga
    18481 ctttcgccag ccccagcgcc agatgtccca gtccacgctc atcgaaatgg gtgtggtgaa
    18541 tgaatgtgga attctccgcc gccgctaacg ttaacggtgt agaacgcgag ccaggggcga
    18601 tacagatgtg tctgacgccg tgacgcgtta atgcttccag aatgaccgcc gcccagcgtc
    18661 ggttaaatgc gcttactgac atgagtttgt ccggtatcaa tattgcggct aagtataagg
    18721 agctacaaaa atagattatt gatatgaatc ggtaatgatg cgactcatta ctattccatc
    18781 tgtaataaag tacgcagccc tgccgctttg ttgtcgattt cctgccactc ttgctcgggg
    18841 tcggaaccac ggacaatgcc cgcgccagca tataatcgca cgacattgcc gctaattttt
    18901 gctgagcgca gggaaacgca gaattcgctt tgttgtaatg agagatagcc cgcagaaccg
    18961 gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa actgtcgcgc cagatcgcgc
    19021 ggtaagccag caactgctgc cgttggctgc aactgatgta aacagatcac atcatccgct
    19081 ttgttgagtg aagtccagat acagcggcga agatgctgca ctttacgcag acgcagtacc
    19141 tgcggcggta aaacatccag cgtctgggta tcggcctgta atcgttggca gatatcttcc
    19201 accaccagca tgttctcgcg ctggttttta tcatccgcca tcagccactc tcctaactgc
    19261 tgcgcctgct tatcatcagg attatttgct actgttcccg ccagcgcttc agtacgcagc
    19321 gctttgtcac gccgccgcca taaccgttcc ggtgaagagc caagaaaagc attttcgcca
    19381 tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc gacgactggc agccatcatc
    19441 gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg cccgagcgag caccactttg
    19501 tcgagctcac cttcggcgat ggttttcgtt gccagttcga ttaattgcgt ccagcccgtt
    19561 ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta acccaggcaa gggcttgata
    19621 ctcaccagtg tggcgataaa ttcttttgcc tgaatcgcat cgtgctgaag ggagctttcg
    19681 ctgaatagcg tcagccgcag cgtggcttta ccgccacagc gtcgccattc caggcggggt
    19741 aaaagtaaat tgccctgcga cgggtcaaaa gcattcagcc cccaaatgcg taagtcggcg
    19801 tgttccgggt gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa acgggtaatc
    19861 gcgcccagga cggcagcttc ttcatcacca ttacgttgtt gccagtagaa ttgcgggtac
    19921 gtttgctgac tggccagcca gctcaaggca tcaaaagcgt ctttgagagg gaaaggaata
    19981 tcgataaccc gaatgccagg tgtcgccgga atctcttgcg acaaatggcg cagtagattt
    20041 tccagcgccg tagtaagtga ttgcacgcgg acctctccct gttaaaagcc tcacattata
    20101 cggggtacta caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc
    20161 aacagtccta gtaccagccc aacggccgcg cccacaccaa ttccttgcca gggtttttcg
    20221 tggacgtagt catcagcacg ataaactgcc tgcttcgccc gatagtaata actgtctgaa
    20281 gcctggctaa cccgtttttt cacatcatcc agcgcttttt ctgcacgcgc tttcagctca
    20341 acatatttct gatcggcggg atcgccagag gagcggagca cctcttccag tgtttcacta
    20401 agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact gattagacat tctcgttctc
    20461 catttgcgta aaacctgtca ctaactatag acaagggttg ttgattacgc ctgaattacc
    20521 tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat agacctccgt cacagggata
    20581 aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg ccccgaggta aacaggctta
    20641 tcaggccagt gatgcgtaca tgtttcgagt gttttactca tcagctgctg ccccactttt
    20701 tcgccacgca acgcttcact gacaatcacc cgacctataa cgaccggctc aagatcatca
    20761 tcacttttca gaatcctcgc atacgccacc agttcatcat ttttccaacc gagaatatgg
    20821 cgattatccc ccgtcaaatc atcaccatcg atatcctgat aagggcaatt ctgctcgaca
    20881 acaaacaccg cgcaacgcag ttgtaataag gcataaagct gggaaaccga taattcagag
    20941 tgatgcagat cttgccattc aatcataatg ctctcctttt agatgtatgc cttatactag
    21001 ccggaatatt ttttgaaacg tgatgaactc atggaattaa tttttttagg tacttcagcc
    21061 ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc ttaacctgca acatccgacc
    21121 cagagcggac tttggttgtt tgactgcggt gaaggcaccc agcatcagct actgcatacc
    21181 gcctttaacc ctggaaaact ggacaagatt tttatcagtc accttcatgg cgatcatctt
    21241 tttggtttac ccggcttgct gtgcagtcgt tctatgtcag gcattatcca acccttaacg
    21301 atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg cgctgcggat tagcggctca
    21361 tggaccgatt atccgctgga aattgtcgaa attggcgctg gcgaaattct cgatgatggc
    21421 ctgcgcaaag taaccgctta tccgctggaa cacccactgg aatgttatgg ctatcgtatt
    21481 gaagaacatg ataaaccggg tgcattaaat gcccaggcat taaaagctgc tggcgtgccg
    21541 cctggcccac tgtttcagga attaaaagcg ggcaaaacaa tcacgctgga agatggaagg
    21601 cagattaacg gcgcagatta cttagctgct ccagtgccag gtaaagcgct cgctattttc
    21661 ggcgataccg gcccctgcga tgccgcactt gacctggcta aaggtgtcga tgtcatggtg
    21721 cacgaagcga cgctggatat aaccatggaa gccaaagcca atagtcgcgg ccatagctct
    21781 acacgccagg ctgcgacact agcccgtgag gctggagtcg gtaagctaat cattacccac
    21841 gtcagctcgc gctatgatga caaaggttgt cagcacctgt tacgtgaatg caggtcaatt
    21901 ttcccggcga ctgaactagc gaatgatttc accgtgttta acgtttaact ctgcttatta
    21961 ttaaaaacag ggcgaaactt gccctgttat cgcaacccgc gctacatata tccacccaca
    22021 cgggcatatt gagcgatttt tgatatttat tcatctcctt acgaactata ttcagagcaa
    22081 taagttgcct ctttttatct tcattcagca caacctttgc aactaaaagg agcccatgat
    22141 ggttacagtt gtcagcaatt attgtcaatt atctcaaacc caactcagtc agacatttgc
    22201 agaaaaattt actgtgaccg aggaattact gcagtcttta aaaaaaacag cgttatccgg
    22261 agatgaagaa agtattgagt tactgcataa tattgcgtta ggttatgatg aatttggaaa
    22321 aaaagctgaa gatattcttt accatattgt tagaaaccca acaaatgaga ccctatcgat
    22381 tatccgactt attaaaaatg cctgtttaaa attatataat ttggcacata ccgcaaccaa
    22441 ctcccacctt aaaccaactg gtccggataa ttcagatgtt ctcctgttca aaaagctatt
    22501 ctccccttcg aaattaatga caattatcgg tgatgaaatt cctcttatat ctgaaaaaca
    22561 gtcgctttca aaggtgcttt taaatgatga gaataatgaa ctgagtgatg atacaaactt
    22621 ctgggataaa aatcgtcaat taaccacaga tgaaatagct tgctatcttc agaagatcgc
    22681 cgctaatgca aaaaatactc aagtcaatta tcctactggt ctctacgtcc cctactccac
    22741 cagaactcac ctggaagacg ctctcaatga aaatattaag agcgatccat catggccgaa
    22801 tgaagtccag ttattcccca taaatactgg cggacactgg atattagttt cgctacagaa
    22861 aatagtaaat aaaaaaaata ataaactaca aataaaatgc gtcatattca actcattgcg
    22921 tgcactaggc catgataaag aaaattcact tatgcgtgtc attaatagtt ttaattctga
    22981 actcatggga gaaatgtcga ataataatat aaaagttcat ttaaatgaac cagagataat
    23041 atttttacat gccgatcttc agcaatactt aagccaaagt tgcggtgcgt ttgtgtgcat
    23101 ggcagcccag gaagtgattg aacaaaggga aagcaattct gacagcgccc cctatacgtt
    23161 attaaaaaac tatgctgaca gatttaaaaa atattcagca gaagagcagt acgaaattga
    23221 ttttcaacat cgacaggtaa acagaaattg ttatttagat aaatatggcg atgcaaatat
    23281 caatgattat tatagagact tagaaataaa acactcacaa ccccaaaata gagcatcagg
    23341 caaaagagta agttaacatc atagttaatt aattcgacca gtcagcaaat atgctgactg
    23401 gtcgaacata gtttgctaaa aagtcatcac tgactgatgt cagtcacacc atccgccagt
    23461 tcaatcttcg ggtatttcca gcgtatctcg gcacgcgatt gctggtctgt tgatgtagtg
    23521 gattgttgtt gctgactttt cttaccctca gagttctgcc ccctgtcaac tcaatctgcc
    23581 aataaataac ataattatat ttttccttat atgaattcct ttttaaaata gcgaagccac
    23641 tgtaaaagat actttttgta tagatctaac aaccgtaata aaacaaacac tcataactca
    23701 ttataaataa tgataaaata acgtctttta ttgataattt atatatgaaa aaaaatcctt
    23761 ttgttatatt atttctgatt tcactccctc tgattatttt taccaaccct gggtcactgc
    23821 attatcgcca acgccccgac atgtcagtcc tgagcaatta gaaaaaacag ttcgttatct
    23881 tacacaaact gtgcatccac gtaatgccga caatattgat aatctgaata ggtccgtaga
    23941 gtatataaaa gagttctttg tcagtagcgg tgccagagtt accgcgcagg gcgtccccga
    24001 ttggctatta cgactctgcg cctggtagcc aggattaccc ttacccg
//
LOCUS       DAEVZA010000072        23991 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEVZA010000072 DAEVZA010000000
VERSION     DAEVZA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.072"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(164..677)
                     /locus_tag="LAF92_002886"
                     /pseudo
     CDS             complement(164..677)
                     /locus_tag="LAF92_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(738..842)
                     /locus_tag="LAF92_002887"
                     /pseudo
     CDS             complement(738..842)
                     /locus_tag="LAF92_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(844..1167)
                     /locus_tag="LAF92_002888"
                     /pseudo
     CDS             complement(844..1167)
                     /locus_tag="LAF92_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1913..2890
                     /locus_tag="LAF92_002889"
     CDS             1913..2890
                     /locus_tag="LAF92_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI9791866.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3175..3915)
                     /locus_tag="LAF92_002890"
     CDS             complement(3175..3915)
                     /locus_tag="LAF92_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI9791867.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALVGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4036..4224)
                     /locus_tag="LAF92_002891"
     CDS             complement(4036..4224)
                     /locus_tag="LAF92_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HBI9791868.1"
                     /translation="MESEIFSCLLRASAGFFFRFCLSWHYPNSPKNGKPALSPHHDWL
                     KTGSQRQNAPLCIWFYPQ"
     gene            complement(4598..5500)
                     /locus_tag="LAF92_002892"
     CDS             complement(4598..5500)
                     /locus_tag="LAF92_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antimicrobial resistance protein Mig-14"
                     /protein_id="HBI9791869.1"
                     /translation="MSIKNIKRIITAWKPSTFETYKKTFEKYGGSVNMHPDVVSYFMI
                     HHDWKFDFFHYEKDGDIKGSYFLCNGKQIGIMARRSYPLSSDEVLIPFSPHARCFFPD
                     KTNKLSIINKQNIINATWKIARKKQNCIIKESFSPKFEKTRRNEIQRFIRNGGEIKCI
                     SQLSDKEISSSYISLFHSRFGGTLPCYEYDNLLMFISHLRELMFGHVLFWDNKPCAID
                     IVLKSESSCNVYYDVPNGAVLNDENCMKLSPGSVLMWLNIDKARRYCQDNNKKMIYSI
                     GAFRPEWKYKLLWSVPCKVGKCLC"
     gene            complement(5569..6678)
                     /locus_tag="LAF92_002893"
     CDS             complement(5569..6678)
                     /locus_tag="LAF92_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004149557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI9791870.1"
                     /translation="MKLLLLTGATGFLGGAVLDKLLDNCNNINLLLLVRAPTPQAGLE
                     RIKENMRKFNVCEERLHALTNDNILPGDLNNPEAFLMDPRLDEVTHVINCAAIASFGN
                     NPFIWNVNVTGTLAFARRMAKVAGLKRFLHVGTAMSCTPHTGSLVKEESASSETGEHL
                     VEYTHSKATIEYLMRKQCPDLPLLVARPSIIVGHSRLGCLPSTSIFWVFRMGLMLQKF
                     MCSLDDKIDVIPVDYCADALLMLLESSLINGEIVHISAGKESSVTFSAIDEAVARALN
                     CDPVGDRYTKVSYDILAMSRHDFKNIFGPCNERLMLKAIRLYGAFSMLNVCFSNDKLL
                     SIGMPKPPKFTDYIKYCIETTKHLSIQQQMEVDFK"
     gene            complement(7111..8064)
                     /locus_tag="LAF92_002894"
     CDS             complement(7111..8064)
                     /locus_tag="LAF92_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HBI9791871.1"
                     /translation="MYLKILATALSAPVAFAALASDTGLSFTPEKISTEIDFGTLSGK
                     AKERVYLPEEKGRKASQLDWKYSNAPIVKGAFNWDLLPRVSVGASGWTTLAGRGGNMV
                     DRDWLDTSNPGTWTDESKHPNTRLNFANEFDLNIKGWLLNQPDYQLGLMAGYQENRYS
                     FTAKGGSYIYSSEGGFRDETGSFPDGERAIGYKQHFKMPYIGLTGNYRYDSFEFGGSF
                     KYSGWVKASDNDEHYNPEKRITYRSDVNNQNYYSVSLHAGYYITPAAKVYVEGTWNRI
                     TNKKGDTSLYSRNLNISDHTKNGAGIESYNFMTTAGLKYYF"
     gene            complement(9337..9495)
                     /locus_tag="LAF92_002895"
     CDS             complement(9337..9495)
                     /locus_tag="LAF92_002895"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI9791872.1"
                     /translation="MAQNAFIESFNRTYRTKILGFCLFRTLDEKRELAANWLSEYNSE
                     RHINHLTI"
     gene            9679..10878
                     /locus_tag="LAF92_002896"
                     /pseudo
     CDS             9679..10878
                     /locus_tag="LAF92_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    9960..10076
                     /locus_tag="LAF92_002896"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(11026..11351)
                     /locus_tag="LAF92_002897"
                     /pseudo
     CDS             complement(11026..11351)
                     /locus_tag="LAF92_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            12332..13519
                     /locus_tag="LAF92_002898"
     CDS             12332..13519
                     /locus_tag="LAF92_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBI9791873.1"
                     /translation="MLMKYISHRAIICTLTLLIIIITVLFTFLRSSDVPEYITAPVRK
                     GDIENSVLATGRIDAIERVNVGAQVSGQLKSLKVKQGDHVTKGQLIAEIDDLPQCNDL
                     RNAEAALNEVKAELQSKQALLKQAELRFKRQLRMLRENASSHEDFESAEAMLATTRAE
                     LHSLNAKLVQAQIEVDKKKLALEYTRVVAPMDGIVIAIVTQQGQTVNSNQSAPTIIKL
                     ARLDVMTIKAQISEADITRISVGQKARFSIFSEPDKHYSATLRAVELAPESVMKDDSL
                     ASNTSASGSGTSNASVYYNALFDVPNPENRLRIAMTAQVTLITDEAQNTLLVPIQAVH
                     RNEGKKQQVLVLAADGRLEPRNVKTGITNSVDIQILEGLNVGENVVLSLPDKKEPEER
                     IML"
     gene            13516..15456
                     /locus_tag="LAF92_002899"
     CDS             13516..15456
                     /locus_tag="LAF92_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HBI9791874.1"
                     /translation="MKKLIELKGVSRTYGNGDQTRTVLKNVDLTIVAGEMVAIIGASG
                     SGKSTLMNIMGCLDVPNRGDYYIDGQNAACLSPDELARVRREHIGFIFQRYHLIPDLS
                     ALGNVEIPAIYANSERDSRRQRATALLGRLGLEGREHHKPCELSGGQQQRVSIARALI
                     NGGKIILADEPTGALDSQSGQEVLAILNELNRRGHTVVMVTHDMKVARHAKRIIELCD
                     GEIIADSGGCVSATETLPKTNRIRQSYWKTLLDRTRESMQMALKAMKTHRLRTTLTMI
                     GIVFGIASVVTVVALGEGARQETLEEIKSLGTNVVSIYPGQDLFDDSIESIRTLVPAD
                     ANALAKQGFIDSVSPEVSASDNIRFLGKSAIASINGVGREHFRVKGIELLQGTTFRDD
                     RNALQEVIIDENTRKAIFDNTGLQALGQIVFLGSVPARVVGIAKSNNRSDASNRITVW
                     MPYSTVMYRIVGKPVLTGISVRLKDNVDNEAAISAISQLLTRRHGIKDFQLYNFEQIR
                     KSIEHTSMTFSILILMVACISLMIGSIGVMNIMLISVTERTHEIGVRMAVGARRSDIM
                     QQFIIEAVLVCLIGGALGIALSYITGALFNALADGIFAAIYSWQAAVAAFFCSTLIGI
                     IFGYLPARKAARMDPVISLASE"
     gene            15460..16830
                     /locus_tag="LAF92_002900"
     CDS             15460..16830
                     /locus_tag="LAF92_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HBI9791875.1"
                     /translation="MKILSSLTLCLITALCSGCTNVLKSDYRAPEVNYPINWTKGDVD
                     GNTSPFDWEEFNDPNLDNWLHLVMTSNNDIAIAALRIHRAQLDAERTGITNTPALKAA
                     LSMDGKKQLNNSSGWAKSGSASLGTSYELDLWGKIARQRDVAEWAVHASEEDFRSARL
                     MLLSEASNNYWRIGFVNQQITTLQQSIDYAKETLRLAEVRYRAGNISSLDVIDAQQNL
                     LTQENQLTGLQREHSQLLNQQAVLLGTVPGCQIVEPTTLPKGSLPKVNANIPASILMR
                     RPDISAKEWQLREALATVDIKRSEYYPTFNLTGALGTSSASLLALLHNPVGSVGANLT
                     LPFLEWRQRDIEVKIARNDYEQRVLEFKQLLYKAMSSIEDALSFRNQLLLQETRLREE
                     LELARKSEWLNEVRYRHGAVRISFWLDAQEKRRQAELRLDENRFNQLQNLAKIYLEFG
                     GASTFP"
     gene            17304..17498
                     /gene="tnpA"
                     /locus_tag="LAF92_002901"
                     /pseudo
     CDS             17304..17498
                     /gene="tnpA"
                     /locus_tag="LAF92_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
     gene            complement(17627..18568)
                     /locus_tag="LAF92_002902"
     CDS             complement(17627..18568)
                     /locus_tag="LAF92_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791876.1"
                     /translation="MAIINIYSKRQRKIRGEVNDVYQYNNIPHALRVQIIKIITDSIG
                     FPSSNERYTSYRNEADKVYAYIHEILSKEYGVFSLKEFAKNDFDALVDFFLKERNTEK
                     CLDFIEICFQILVSHVAKNHYEFKDITSQSPGDAVIELNERFREHGVGYQFESEEIIR
                     IDSQLIHADVVKPTLILLSGEPLFEGANDEFLAAHEHYRHKRYKECLNDCLKSFESIM
                     KAIHDKNNWKYSPNDTASKLINSCLSQNLIPAYLQSQFTSLKTMLETGIPTIRNKNAG
                     HGQGADIKEVPEELVSYMLHLTATNLLFLLKCEKNIK"
     gene            complement(18803..20192)
                     /locus_tag="LAF92_002903"
                     /pseudo
     CDS             complement(18803..20192)
                     /locus_tag="LAF92_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(20502..20651)
                     /locus_tag="LAF92_002904"
                     /pseudo
     CDS             complement(20502..20651)
                     /locus_tag="LAF92_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(20829..22022)
                     /locus_tag="LAF92_002905"
     CDS             complement(20829..22022)
                     /locus_tag="LAF92_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBI9791877.1"
                     /translation="METTSVIILNGFLGAGKTTLLKNLLIQAHEKQLSVSVIVNDMSE
                     LDVDGVLIANTDIVDAKDNNFVSITADSISSPAGLKKMDLAINHLLDHNKPDVMLIET
                     SGSSHPLPLVKYLRRHSRVRLKAFLTLVDTVMLHEDYNDGKKLIPTLQENLRHNKRGL
                     ENLLAEQIMFCNRLLLTRNDRLPFDIISAVAKAIHPLNPSVDVLAVSWGNIELSTLLA
                     IPDYNFDRVELLISELEALVGNMDTPCNNEELIWRVIRDDRPFHPQRLWDTCHRFMGM
                     GVYRSKGFFWLPGRDDLALLWNQSAGSISLALIGYWKAGVLEHTDNNLTREERSALQR
                     HIDTASGRFGDRCCQLTIIGNATEVNDFTHALSLCLLTEEEIQWWMSGGVFPDPWPQK
                     VTRLS"
     gene            complement(23264..>23991)
                     /locus_tag="LAF92_002906"
     CDS             complement(23264..>23991)
                     /locus_tag="LAF92_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI9791878.1"
                     /translation="RELPTYGYRRVWALLRRQAKLDGMPAINAKRVYRIMRQNALLLE
                     RKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAV
                     TTGGFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPK
                     STAVRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGY
                     RSPREYLRQRACNGLSDNRCLEI"
BASE COUNT         6547 a         5442 c         5347 g         6655 t
ORIGIN      
        1 gctttttcgg ttgaaaagaa attgattgaa ttactggaaa aacaatctgg tctccatacg
       61 tctgtggatt gcagtactcc ccttacagag cagagtctgt cgagaaatga cggtgaagac
      121 cagaccagaa gaaacgctga aaatatgttc ggagatgact gaactaaccc agcctggcca
      181 ggggaatatc tcgccattta gttgtataac actgactcaa catttcccgt ttcatttgcc
      241 attcaggggc ggttcctcgc cccgcaaacc acactttccc ttttcccgac tggttcagtt
      301 catcgagtgt tttcattaac ttttcactgt ttttacgggg ctggatttca tcaaataatc
      361 ccggctgcgc tatacccgat ggtgtgaaat cagccagcat gactcctgcc ttcatatagc
      421 ggtacccttc acgccagaca tggtttaagg ctctgcatgc ggcggcaata atgtcccggc
      481 tgtcctgtgt gggcaatgaa agcttttcca cagcggcatt gctgtaacag ggttcagggg
      541 gatgtccgta caaatgtcgt cacctgccag caatactgac gctccccacg tagtttctct
      601 gcggcccgct ctgcatactg aacaacagcc tggtgcatgg catctttgtc tgtgattcgt
      661 tcaccaaaac tgcgactatg tcaacgccac gatgtttgac cgttatttgc cattttcatg
      721 cccgctaaat actttcgtta ctgatgctgt ccgctttgca ccatttcatc atcactgatt
      781 ggcgttgtgt ttttggtcag cactccagct ttacgtttct cttttaaccg gtaactctct
      841 ccttgttctt tttgacggac gcattttacg tttgggctgg atgtagtaac gcagcatgga
      901 acgtccgccc gtataaccca ttgccttaat ctccgcgaag ataacctcgc tattccagac
      961 attctctgcc aggcgcatgt cgatgtaatc cataaacggt ttcagcttaa ccattttgtg
     1021 gcgggtcttt ctggctggcg gttcagggta tttgaggtag cgtctgaccg tccgttcaga
     1081 gcaaccaatc tgagccgcaa tatcgacaat gtacgcgccc tgctggcgca tttgctttat
     1141 catgtaaaag tcctctctgc tcagcatgtt gatgtccttt ctggtgtgag aaccttaagg
     1201 aaacaacatg ttgggtggag cggacaatcc aaatggtgaa ttaccgtctt atatcactgg
     1261 cgctgacacc tgtagtttgt atacacaatc cctggcattg aaacacacat atgaagtgta
     1321 cttcggatat gaccgtacgc tcaggaagta cggcagattc cgtcaaaaat tacctgtaaa
     1381 gacagacatc agaaaaaaca gtgctcgccg cagaccggtg accgggcgaa gcccgcgaac
     1441 cggcgaggaa gcgcaatgta tctgtagtta cagggagcag gtacgccctg aagccctttc
     1501 ataaaacggt tgtgtgcgct gtccttctgg aatgatttat ccacatatcc ggaggcccga
     1561 tccagtaaac agatccatga atgatcaaca aaggatccat taaagatccc catatcgctg
     1621 caaaccttgt cactcatggg ccgggaccac gatcacataa gctgtggcat gttactgata
     1681 aactgtaaca tgctaatgat aagctgtatt tagtaatcca tatactgaag taagttaatg
     1741 acataaacta tggtcagtat gccagactca gttgttaaat acaggctgca ggtttttctt
     1801 cagtcagtta gcggagttct gacacacgat tttctgttta ttcttttact gtccacaggc
     1861 tggaggcttt ctggaaaacg aaaattcaga catcaaaaaa ctgttcggcg aggtggataa
     1921 gtcctccggt gagctggtga cactgacacc aaacaataac aacaccgtac aacctgtggc
     1981 gctgatgcgt ctgggcgttt ttgtaccgac ccttaaatca ctgaagaaca gtaaaaaaaa
     2041 tacactgtca cgtactgatg ccacggaaga gctgacacgt ctttccctgg cccgtgctga
     2101 gggattcgat aaggttgaga tcaccggccc ccgcctggat atggataatg atttcaagac
     2161 ctgggtgggg atcattcatt cctttgcccg ccataacgtg attggtgaca aagttgaact
     2221 gccttttgtt gagtttgcaa aactgtgtgg tataccttca agccagtcat cccgcaggct
     2281 gcgtgagcgc atcagccctt ccctgaagcg cattgccggt accgtgattt cgttttcccg
     2341 taccgatgag aagcacaccc gggaatatat cacccatctg gtacagtcag cttactacga
     2401 tactgagcgg gatattgtgc agttacaggc cgatccccgc ctgtttgaac tgtaccagtt
     2461 tgacagaaaa gtccttctcc agcttaaggc gattaatgcc ctgaagcgac gggagtccgc
     2521 ccaggcactc tacaccttta tagagagcct gccccgggat ccggcaccga tatcgctggc
     2581 gcggctgcgt gcacgcctca atctgaagtc tcctgtattt tcccagaacc agacggtcag
     2641 acgggcaatg gagcagttgc gcgagattgg atatcttgat tacacggaga tccagcgggg
     2701 gcggacaaaa ttcttctgta ttcactaccg gcgtccccgg ttaaaagcgc cgaatgacga
     2761 gagtaaggaa aatccgttgc aaccttcacc tgcggaaaaa gtcagtccgg agatggcgga
     2821 gaagcttgcc ctgcttgaga aactgggcat cacgctggat gacctggaaa aactcttcaa
     2881 atcccgctga acataaactg tagtcagtga agagcgctcc tttactgact acagcttata
     2941 ttaaccggtg cagtgagtgg tctgctcact gcagtttata ttctgtttcc tgcagtgctg
     3001 tctgtagctg agctgccatc tgcctgtccc ttacgtaagt gatcccgtaa cctgatgctg
     3061 aggcattgct cccttcataa aacatgactt actcactaca gcttatatac atgctccagc
     3121 ttatgttatg tctgttctgc tgaccacagc ttatataagg aagcgcgtat gccgtcacct
     3181 caggggaggc agtgtacgca ggatctccgc agcatcccgc ccgtcacctg tgaaaggcac
     3241 tgccagcgtg gcagccatat ccagtgcaaa cactctggta taaacctcca tcgaacgtgg
     3301 atccctgtga ccaaccagtg cctggatgac tttccggggc tggcggtgat agagcatgtg
     3361 catgatatag ctgtgccgga aggtgtgtgg tgtgaccgga atcgaaaagt gtactccgtc
     3421 agcttcggcc cgtctgacag cctgcttcag ccagttgcgc atggtttcgt cggtcacggc
     3481 ccataatggt tcacgacgac ggggccgggt ggtgatcatc cagctttcca tctgcctgac
     3541 atagcttata tctgtcagcg gaaccaggcg cacttcatct tttggcgggc gtccgcgtcg
     3601 cgcacgcact ttttcggaca ggatccgcac aaacggtctt actccattca ggtcaaatga
     3661 ttccggcgtc agcatccggg cttcgccaat acgcattccg gtattccaga gggtggcgaa
     3721 cagcatatgg tgacgctgat ccggcatata gaagagaagg gcactcactt ccggggccag
     3781 caggtaggcc ggtgtggccc ccgtggaagt ggccattctt ctcagggaga gcgctgttgc
     3841 aaaatccacc cccggcgcaa tgggtaacag ggagacgcgt tcaggtgaat tctgcagggg
     3901 aatgacattg ttcatgagtt atttttcacg tagcacacgc actgtttatg gatacaggta
     3961 ttctgacctg aacgcccgga tccttccacg acaataaatc gcagcgcgcg gatccttttg
     4021 tttgattatc acgcattatt gcgggtaaaa ccaaatacat aaaggcgcat tttgccgctg
     4081 ggatcccgtc ttaagccagt catggtgcgg gctgagggct ggtttaccgt ttttgggaga
     4141 attggggtaa tgccaggaca ggcagaaacg gaaaaaaaat cctgcagacg cccttaaaag
     4201 acaactgaaa atttctgact ccatattcct ctggtttcag actgcttaat cacatcatca
     4261 acctgataca taataaacag attctgttca cagaatgaac aattaagtaa atggttagat
     4321 tatgcgaaaa agtacagata tttctcacca tcgttattga ataatatgtc ttcacacaaa
     4381 caatattaat attttgagtg taagccctga ctcaaagaaa gaaatgtctg ttttcggaat
     4441 caaaaatata tagttatcaa aatacattaa tttcaaattt gaaattaatg taaaacgatt
     4501 tagtaaatgt tatgacatta taaatctact cctccatgtt tagaggagta tttgcagact
     4561 ggacgctctt atatagttac ttacctttat acatattcta acataagcat ttaccaacct
     4621 tacagggaac agaccatagt agtttatatt tccattcagg tctgaatgca ccgatagaat
     4681 atatcatctt tttattatta tcctgacaat accttcgtgc tttatctata ttcagccaca
     4741 taaggacact tcccggactt agtttcatac aattctcatc attcagaact gcaccatttg
     4801 gaacgtcata ataaacatta catgagcttt cagatttcag tactatatca atagcacacg
     4861 gtttattatc ccagaatagc acatggccaa acataagttc tcgtaagtga gaaataaaca
     4921 ttaacagatt atcatattcg tagcaaggta gggtacctcc aaaccgggaa tgaaaaagtg
     4981 atatataaga gctggatatt tctttatcgg ataactggct tatacactta atctctccgc
     5041 cattcctgat aaatctctgg atttcatttc ggcgagtttt ttcaaattta ggtgaaaaag
     5101 attcctttat aatacaattt tgtttttttc tggctatttt ccatgttgca ttgataatat
     5161 tttgtttatt tataattgat aatttattag ttttatccgg aaaaaaacat cttgcatgtg
     5221 gggaaaaagg aattaatacc tcatcagatg aaagaggata agaccttctt gccattatcc
     5281 caatttgttt tccattgcac agaaaatatg agcctttgat atcaccatct ttttcatagt
     5341 gaaaaaaatc aaacttccag tcatgatgta tcataaagta tgaaacaaca tccggatgca
     5401 tgtttacact accaccgtat ttttcaaaag tttttttgta cgtttcgaat gtagatggtt
     5461 tccaggccgt aataatgcgc tttatatttt taattgacaa agtcatatcc tcggcgtttt
     5521 tatatgttat gagtgcaaca ccaacaataa ttctgattat gataaatatt atttaaaatc
     5581 aacttccatt tgttgttgaa ttgaaaggtg ttttgtcgtt tctatacagt atttaatata
     5641 atcagtaaac tttggcggtt taggcattcc gatactcagt agcttgtcgt tactgaaaca
     5701 aacattgagc atactgaacg ctccatataa acgaatggct tttaacataa ggcgttcgtt
     5761 acagggacca aaaatatttt taaaatcatg acggctcatt gccagtatgt cataactgac
     5821 tttagtatat ctgtctccaa caggatcaca gttcaaagca cgggctacag cttcgtcaat
     5881 agcagagaac gtcacactac tttctttacc tgctgatata tgaacaatct caccattaat
     5941 taacgaactt tcaagcaaca ttagcaatgc atcagcacaa taatctacag ggataacatc
     6001 tattttatca tccagagagc acataaattt ttgcaacatt aaccccattc tgaataccca
     6061 gaaaatactg gttgaaggta agcaccctaa acgactgtgg ccaacaataa ttgatggtcg
     6121 ggcaaccaac aaaggtaaat caggacactg cttacgcatc agatattcta ttgttgcttt
     6181 tgaatgcgta tactccacta aatgttcacc tgtttctgat gaagcagact cttccttaac
     6241 tagcgacccc gtatgaggtg tacaagacat agcagtacca acatgaagga agcgtttcag
     6301 tcctgccact tttgccattc ttcttgcaaa agcaagtgta cctgtaacat tcacattcca
     6361 tataaaagga ttattaccaa aagaagctat agccgcacag tttataacat gagtgacttc
     6421 atcaagacga ggatccatga gaaaggcttc cggattattt agatccccag gcaagatgtt
     6481 atcattagtt aatgcatgca acctttcctc acaaacatta aatttacgca tattttcttt
     6541 aattctttcc agtcccgctt gtggagtagg tgctcgtact aaaagtagca aatttatatt
     6601 attacagtta tccagcagct tatccaggac cgcgccacca agaaatcctg ttgcacctgt
     6661 aagtaataac aatttcatat ctaacctctt ttgttttcat gtaatcatta taaaaataaa
     6721 ttatcttaca taaactatga ttaaacaaaa tcaagagaga ttaaatgtaa aaacaatgtt
     6781 tatttttata cgacaggatc cttcaggtta ttgcgagtgt ttttattgga tggtcttctt
     6841 attattgacg agtaatagaa ttataataaa attcatatta ttaacatcgc ttatcaatta
     6901 tttttatgtc gataacactg tgtcgttatg catgaagagt ggcagagcag catctttaaa
     6961 aatacaagag ttaacagaaa cacttttatc gttgagcaca tataccctcc acatattata
     7021 aaaaatcaaa agaccaccat cagaagtgct ggccttttgt ttatcatatt taaagtgtca
     7081 tgaaaaagtt aaaaacaggc atgcttcagg ttaaaaataa tacttcagac cagctgtggt
     7141 cataaagtta tagctttcaa tgcctgcacc atttttggtg tgatcagata tattcaaatt
     7201 tctggagtac agagaagtat cacctttctt attagtaata cggttccatg ttccttcgac
     7261 ataaacttta gctgcaggtg taatataata accagcatgt agtgagacag agtaatagtt
     7321 ctgattattt acatcgctac gataggtgat ccttttctca gggttgtaat gctcatcatt
     7381 atccgatgcc ttcacccatc cgctatattt gaaactcccc ccgaactcaa agctatcata
     7441 ccgataatta ccggttaacc ctatataggg cattttaaag tgttgtttgt agcctatagc
     7501 tctttcacca tctggaaatg atcccgtttc atcccggaag cctccctcac tactatagat
     7561 ataagagcct ccttttgctg taaagctata acgattttcc tggtaaccag ccataagacc
     7621 taactgatag tctggttgat tcaggagcca tcccttaata ttcaggtcga attcattagc
     7681 aaaatttaga cgggtattcg gatgtttact ctcatcagtc caggttccgg gattgctcgt
     7741 atccagccag tccctgtcca ccatattgcc tccacgacca gctaatgttg tccaccctga
     7801 ggcaccgaca gatactctgg gtaacagatc ccagttaaat gcccccttga caattggcgc
     7861 attactgtat ttccagtcca gctggctggc tttgcgccct ttttcttcag gcagataaac
     7921 ccgttctttg gccttgccac ttaaagtgcc aaaatcaatc tccgtactga ttttctccgg
     7981 tgtaaaagaa agcccagtat cagaagccag tgcagcaaac gctacaggag ctgagagtgc
     8041 agtagcaaga atctttaagt acatagacaa gttaactcct ttccactcgt ttgtttttga
     8101 taaatatttt tttatttact cgtagtagtc aactgaaaaa tgctgttttg tttaacgttt
     8161 gtttacaatg tggccgcatg tcgctttatt tattcccttt tatgtcagaa tatcagggga
     8221 gtcagtcaaa cccggtatga ttactgcccc ggtcatccat gttccaggaa atatcagggc
     8281 atacagggtg ctggtaactg cgaataaggc cctggtcgga cactttatgc cgggaacgtc
     8341 tgtaacatgg aaaccggtac cttcttcctg ggtttatttt gttaactctt atctaaaggg
     8401 agacaaaatg gccaaatcct gcgattatga agcaatcagg gtttttatta gcgttaatgt
     8461 cggcaaatat accaataacg ccattacagt taattattca gatagcgttt gtgcgacaag
     8521 gcattaccat tcgacgaaaa taaactcagg tggttgatat cagaaatcaa ccgtggctcc
     8581 acctacaaaa aaagttgctg gtttataaat ccctcctttc tgattaataa gaaaactaat
     8641 aacatatgag tataattact tgttcagata agttaattat cttcgtgatt gatatttatt
     8701 tggtttcagt aaaaatgatg tttttattat aaagtttata aataccaagt cggtggtgtt
     8761 tattagtgta atggagggga gtgcgtaaat cgttacatgt aacacttcct tccaataata
     8821 ctttataaat caataaatgt atgattactt atttcctgaa atatcacata tattgctata
     8881 tttcttaacg agatattctt attttattga tgattgcatc tcttttattt aatgtaaagc
     8941 tttcttatta atcttctaat gtattgcaaa atggtagatg ctgagtaatc aacagacttg
     9001 tatgtaaagt caagattaac gttgctaaag cacgcatcaa ttttctgata aaaggatgtg
     9061 gttgtttttt tcgataaaaa aaattcttgt ctaaccgctg gtgaaataag attattatta
     9121 ccccggatta atactgagcc taacaataga atacaaaaaa aacaaaatgc tttaaataag
     9181 gggagcacta taagttatcc tctttacttt atgcttacca actcattttg ttactttata
     9241 acataactca tcttgcgttg taaacagact cgttttagtt ccatgcacct tttgagatcg
     9301 tgaccaaata atggcgttag tgatgttctt ccagtgtcat atggttaagt ggttgatatg
     9361 gcgttcactg ttatattctg acaaccagtt tgccgccagt tcccgctttt catccagcgt
     9421 tctgaacaga caaaaaccga gtatttttgt tcggtatgtc cggttaaaac tctcgataaa
     9481 agcgttctgc gccaacttac ctgattgggt aatctccagt tttactacat gtttttctgc
     9541 gcattacgtc cgtgttggat aaaaaaaatc ctggccataa tctatgcgaa tcatagctgg
     9601 atagacatgg ttttccgcaa tccttgaacc gccccgggaa tcctggagac taaactccct
     9661 gagaaagagg taaacaggat gactaaaaat actcgttttt cccccgaagt ccgtcagagg
     9721 gcgattcgta tggttctgga aagtcagggc gaatatgact cacagtgggc ggcaatttgt
     9781 tccattgccc caaagattgg ctgtacaccg gagactctgc gtgtctgggt acgccagcat
     9841 gagcgggata ccggaggcgg tgatggcggg ctcaccaccg ctgaacgtca gcgtctgaaa
     9901 gagctggaac gtgaaaatcg tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc
     9961 gcttattttg cgaaggcgga gttcgaccgc ctctggaaaa aatgatgcca ctgctggata
    10021 agctgcgtga gcagtacggg gtcggaccgc tatgcagcga actgcatatt gccccgtcaa
    10081 cgtattacca ctgtcagcaa cagcgacatc atccggataa acgcagtgcc cgtgcgcagc
    10141 gcgatgactg gctgaagaga gagatacagc gcgtatacga tgtacggtgt gcgtaaagtc
    10201 tggcgtcagt tgttacggga aggaatcagg gtggccagat gtacagtggc gcgcctcatg
    10261 gcggttatgg gactttccgg tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg
    10321 aaagccgttg tcgcaggcga ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag
    10381 ttgtgggtgg ctgattctac ttacgtcagc acatggcagg gggtcgtcta tgtggcgttc
    10441 atcattgatg tgtttgccgg atacatcgtg gggtggcggg tctcatcgtc tatggaaacg
    10501 acatttgtgc tggatgctct ggagcaggcg ttatgggccc gtcgaccgtc cggcacagtc
    10561 catcacagtg ataaaggttc tcagtatgta tcgctggcct acacacagcg gcttaaggaa
    10621 gccggattac tggcatcaac aggaagtaca ggcgactcgt atgacaacgc gatggcggag
    10681 agcatcaatg gcctttacaa agcggaggta atacaccgta agagctggaa aaaccgtgca
    10741 gaagtggaac tggccacact cacgtgggtg gactggtata acaatcgacg attgctggaa
    10801 aggctgggcc atactcctcc ggcagaagca gaaaaagctt attatgcttc catcggaaac
    10861 gatgatctgg cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca
    10921 cctacagagt accgctacca cttgtgaagc tggcagattc agattgtttt caatcgacaa
    10981 cgcctcatag ttaaagtcat caacgacatt gaacgtgtga agtggtcaac aaaaactggc
    11041 caccgagtta gagtttttcc agtatcgatt ttccgattcg tttggggata acccaccgtt
    11101 atattcgtgc ggtcttagtg cgctgtaata tccaacgata tagaccgtta tggcgtgagc
    11161 ggcctcgcag aagcttcgta acccaccacc ggcatccatt cgttcttcag actcctgaag
    11221 aagcgttcca ttgggctgtt atcccagcag tttccgcgcc ggctcatact ctgcctgatc
    11281 tggtatcgcc acaataactg ccggaactgc ctgctcgtat cgctgtggaa catcaccccg
    11341 ccgggcttac ccgtccagat ataggtcaca tcaccgcacc acacctgtat agcggcgtaa
    11401 tccccaaaaa cgatgctggt tgtcagtaaa caagccatga atgacagtcg ttcctgaaat
    11461 cataagacct ggaggagtgt atgctccgtc gcagtaatta ccacgatacg cttgcagaat
    11521 tttttccagt tactgaagcg tgaacggata aagaaaaagc tcttcggaac gcgggaagac
    11581 gcctccaacg atatttttga acatcgaaat gttttataac aataagcgtc ggcatggttc
    11641 ctgcgaacag atgtcaacaa tagaatataa aaacaattat tatcaacaac ttggaaggag
    11701 taagactact cgtggcgatt caatattaga tcattgatgg agatataaat ggctcacttt
    11761 ttatcggttg ttgacgatac gtttatcaag tatacatgaa aatttcttca taccagctat
    11821 atgagacgtt aagactaata atcttactcc caaaatgact atgttgttgc agtttggttt
    11881 attgactctt gtctatagtt caccgaatgc caagatgagg gagcgaggaa ctttactgtc
    11941 tacgcctctc ttcctggtta caaagacaag cgtaaaactc accatccatt agaactaaag
    12001 gcactttcct gagcagagtt ttcatctgca agataaaagc tattttacag ccgtaaagtc
    12061 cagtgaaacg aaagagaggt tagtgaatta attacttgat gtattaaagt ataatgggcg
    12121 tagataacac cttaatacga aatcttgtca ggctttcccg gtaattagta atactgttga
    12181 aacaaagcga gagggggtta gcccaattta cgcaaataaa taataatctt gattatgatt
    12241 aattccaaat aagtatattt ttccagtaga tgctgggcct taactaagaa aacccagaat
    12301 accactttca ttgttatgga aaacatggac aatgttaatg aaatatattt ctcaccgcgc
    12361 catcatttgt accttgacac tgttgatcat tattatcacc gttttgttca cctttctgag
    12421 aagctctgat gtgccagaat atattactgc cccggtgcgc aaaggcgata tagaaaattc
    12481 tgttctggcg acaggccgta ttgatgcaat tgaacgggta aacgtagggg cacaggtatc
    12541 agggcaatta aaatcactga aagtgaaaca gggcgaccat gtgactaaag ggcagttgat
    12601 tgccgaaatt gacgacctac ctcagtgtaa cgatttgcgt aatgccgaag ctgcactcaa
    12661 cgaagtgaaa gcagaacttc aatccaagca ggcgctcctg aaacaggctg aactgcgttt
    12721 taaacgccag ctccggatgc taagagaaaa tgccagctca cacgaagatt ttgagtctgc
    12781 ggaggcaatg cttgcgacca ctcgcgctga attacattct ctgaatgcca aactggtcca
    12841 ggcacagata gaagtagata agaaaaagct tgcccttgaa tatacgcgag tcgtagcgcc
    12901 aatggatgga attgttattg ccattgtcac tcagcaaggg caaaccgtta actcaaacca
    12961 gagtgcccca acaattatta aactcgctcg tctggatgtc atgaccatta aagcacagat
    13021 ctccgaggct gatataaccc gtatttctgt ggggcaaaaa gcccgcttct ctatcttctc
    13081 cgaaccggac aagcactaca gcgcaacact gcgtgccgtt gaactggcac cagaatcggt
    13141 aatgaaagat gactctctcg ccagcaacac ttcagcatca ggctccggaa cttcaaatgc
    13201 gtccgtttat tacaatgctc tattcgatgt gcctaaccca gaaaatcgac ttcgtattgc
    13261 catgactgct caggttaccc tgattactga tgaagcccaa aatacactgc tggtacccat
    13321 tcaggcagtg cacagaaatg aggggaagaa gcaacaggtc ctggtgctgg cagcagatgg
    13381 caggctggaa ccccggaatg tgaaaactgg tatcacgaac agcgtggata ttcagatcct
    13441 cgaaggttta aatgtcggag aaaatgtcgt gctgtcgctg ccggataaga aagagcccga
    13501 agaaaggatt atgctgtgaa aaaacttatt gagttaaaag gggttagccg aacttatggc
    13561 aacggagatc aaacgcggac cgtcctgaaa aatgtcgatt tgacaatcgt tgctggagaa
    13621 atggtggcaa tcattggagc ctctggctca ggcaaatcaa ccttaatgaa cataatgggt
    13681 tgccttgacg tacccaatag aggtgattac tacatcgacg gacaaaatgc cgcctgtctt
    13741 tcacccgatg agctggctag agtgcgccgg gaacacattg gctttatatt ccagcgctac
    13801 catttgatac ctgatcttag cgcacttggt aacgtggaga tcccggccat ttacgccaac
    13861 agcgaacgcg acagccgtcg gcaacgtgca acggcgttgc tgggcagact gggactggaa
    13921 ggacgtgagc atcataaacc ttgcgaactt tcaggcggtc agcagcaacg cgtcagtatt
    13981 gcccgagcac tgatcaacgg tggaaagata attttggcgg atgagccaac tggtgctctg
    14041 gattcgcaat ccgggcaaga agtgttagcc attctgaatg aacttaatcg acgtggtcat
    14101 actgtagtaa tggtgaccca cgatatgaag gtcgcccggc acgcaaagcg aatcattgag
    14161 ctgtgtgatg gcgaaatcat cgctgatagt ggaggttgcg tatctgcgac ggaaacacta
    14221 ccaaaaacca acagaatccg gcaaagctat tggaaaacac tgctcgatcg aacgcgtgaa
    14281 tcgatgcaaa tggcgctaaa agcgatgaag acgcatcgct tacgcaccac gcttaccatg
    14341 atcgggattg tttttggtat cgcctccgtt gtcactgttg tggcattggg tgaaggagcc
    14401 aggcaagaaa cactggaaga gattaaaagt ctggggacaa acgtggtcag catttatccc
    14461 gggcaggatt tgttcgatga cagcattgag agtattagaa cattggtacc tgctgacgca
    14521 aatgcactgg cgaagcaagg gtttatcgat agcgtcagcc cggaagttag cgcttcagac
    14581 aacattcgtt ttctgggtaa atctgcgata gcgtccatta acggcgttgg gcgggaacat
    14641 ttccgggtaa aaggtattga gttgttgcaa gggactactt ttagagacga tcgcaacgct
    14701 ctacaggaag ttattatcga tgagaatact cgtaaggcca ttttcgacaa cacagggtta
    14761 caggctctgg ggcaaatagt ctttcttggt tctgtaccag cacgagtggt tggcatcgct
    14821 aaaagtaaca accgtagtga tgcatcaaat cgcattactg tgtggatgcc ttacagcacg
    14881 gtgatgtacc gtattgtggg taaaccagtt ctgaccggta ttagcgtcag gctaaaagac
    14941 aatgtggata acgaggctgc aatcagcgct atctcccaac tattaacccg gcgtcacggc
    15001 ataaaagact tccagcttta taactttgaa caaataagaa agtctatcga acacacctca
    15061 atgaccttca gtattttaat tcttatggtc gcatgtatct cactaatgat cggcagtatc
    15121 ggggtgatga acatcatgtt gatttctgta acggaacgaa ctcatgaaat tggcgtacga
    15181 atggcggttg gcgcgagacg cagcgacatt atgcaacagt ttattattga ggccgtattg
    15241 gtttgcctga ttggtggcgc actcggtatt gcactgtcgt atatcacagg tgcgctcttt
    15301 aacgctctgg cggacgggat attcgcagct atatattcgt ggcaggctgc tgttgctgca
    15361 tttttttgct caaccttaat tggcataatc ttcgggtatc tcccagcccg caaggcagca
    15421 aggatggacc cggttatttc actggccagc gaataatata tgaaaatttt atcatcgttg
    15481 actttatgcc ttatcaccgc actctgttca ggttgcacaa acgtgctgaa aagtgactat
    15541 cgcgctcccg aagtgaacta tcccatcaac tggaccaagg gcgatgtgga cggaaataca
    15601 tccccgtttg actgggaaga atttaacgat cctaatctcg ataactggct tcacctggtg
    15661 atgacaagta ataacgacat agctatagct gcactgcgga tacatcgggc gcagctggat
    15721 gcggaaagaa caggtatcac caatactcct gcactaaagg cggcactgag tatggacgga
    15781 aaaaaacagc tgaataactc gtccggctgg gcaaaaagcg gttctgccag ccttggcacc
    15841 agctatgagc tggatctctg gggaaaaatt gcccgtcagc gtgatgttgc tgagtgggca
    15901 gtccatgcca gtgaagaaga ttttcgctcc gcgcgcctga tgctgctttc agaggccagc
    15961 aataattact ggcgtatcgg ttttgtaaat caacaaatta ccaccctcca gcagagtatt
    16021 gactatgcaa aagagacgct gcgtctggcc gaggttcgct atcgtgcagg aaatatctcg
    16081 tcgctggatg tgattgacgc gcagcaaaac ctgctcacgc aagagaacca gcttacaggg
    16141 ctacaacgag aacattcaca gctactcaat cagcaggccg ttttgctcgg caccgtgccc
    16201 ggttgccaga tagtcgagcc cacaacgctg ccaaaaggaa gcctgcctaa ggtcaatgcg
    16261 aatattcctg ccagcatact gatgcgccgg cctgacataa gtgctaaaga gtggcagttg
    16321 cgtgaggcgc tagctacggt tgatatcaag cgtagcgaat attaccccac cttcaacctg
    16381 acaggcgctt tgggtaccag cagcgcttcg ctactggcat tactgcataa tccggtcggt
    16441 tccgtaggcg ctaacctgac gctaccgttc ctggagtggc gacagcggga catagaggta
    16501 aaaattgccc gtaacgatta cgaacagcga gtgctggagt ttaaacagct actttataag
    16561 gcaatgagca gtatagagga cgcgctttca tttcgtaacc agttgctgct tcaggagaca
    16621 agactcaggg aagaactgga acttgcacgt aagtcagagt ggttaaacga agttcgttac
    16681 cggcatggtg cagtacgtat ttcattctgg cttgatgctc aggaaaaacg tcgtcaggcg
    16741 gagctgcgac tggatgaaaa ccgcttcaat cagttgcaga atctagcaaa aatttatctt
    16801 gagtttggcg gggcatcaac ctttccttaa gtctaattgg ggacaaccag gagcaataag
    16861 gagtgaaaaa tagccacagc tgaaaacacc atagccattg acatttgagc tttacctgct
    16921 cttgaaaaca aaaaatcact gttaagctca atgctattca cttcagcaga gcagtattgc
    16981 gtgtcacgag catgtttacc attctgtgta aatatctttt cttaaaagtg atcatcaaag
    17041 cagtcatcaa actcgataaa tatccaccgg catagcgtaa tgccgagcaa ttaaggatca
    17101 attgaccgct ccttaaactg cggcactata acggcttcta caacagggag ccgttttctt
    17161 gtgcatagaa aacccccagc taggctgggg gttccggaaa gctttcagct ttgagccagt
    17221 tattaaaacc ccttttgatt tattaaaaca ccttgcggtc tggcaactgc aagtgtcaaa
    17281 caagaaatca aaagggggtc ccaatgggga acgaaaagag cttagcgcac acccgatgga
    17341 actgtaaata tcacatagta tttgcgccaa aataccgaag acaggtgttc tacagagaga
    17401 agcgtagagc aataggctgt attttgagaa agctgtgtga gtggaaaagt gtacggattc
    17461 tggaagctga atgctgtgca gatcatatcc atatgctaca atgtttgtca aagctcagtg
    17521 aaaatgtcta atcactggct cattataaat gtccatatat tgaaaaggcc gctgcgaaaa
    17581 tccctcccgc agcggcctct ttagcataac gtcattatgc gaaccatcac ttaatgttct
    17641 tctcgcattt taagagaaat agtaaatttg tagcagtaag atgaagcata tagcttacca
    17701 attcttccgg aacctcttta atatcagctc cttgcccatg acctgcattt ttgtttctga
    17761 tggttggaat tcctgtttcc agcattgttt tcagacttgt gaactggctt tgtaaatatg
    17821 ccggaattaa attttgactt aaacagctat ttattagttt tgatgctgta tcattaggac
    17881 tatatttcca gttattctta tcatgtattg ctttcattat actttcaaat gatttcagac
    17941 aatcatttag acattcttta taacgcttat gcctgtaatg ctcatgcgca gcaaggaatt
    18001 catcattggc gccttcaaac aagggttctc cacttaaaag aatcagggtt ggctttacga
    18061 catcagcatg aatcaattga gagtctatcc ttattatctc ttcagattca aactgataac
    18121 caaccccatg ttctctaaac ctttcattta gttcaataac tgcatctcca ggactttgac
    18181 ttgttatgtc tttgaattca taatggtttt tcgcaacgtg gctcactagt atctggaagc
    18241 atatttctat gaaatctagg catttctctg tatttctctc tttaaggaaa aagtcgacaa
    18301 gagcgtcaaa gtcattcttt gcaaattcct tcaaagaaaa aacaccatat tccttagaaa
    18361 gtatctcatg gatatatgca tatactttgt cagcttcatt tctatacgat gtgtagcgtt
    18421 cattagaaga ggggaagccg atagaatcag ttattatttt aataatttgc accctgagtg
    18481 catgtggaat attgttatat tgatatacgt catttacttc gccacgaatt tttctttgtc
    18541 tcttagaata aatattaata attgccatga aatagcctca agattttttt acatgctgtt
    18601 ctgtgatact tgctgtttgg ctctttgcac cgcgaagcgg ttcggggttc tgtgacaccc
    18661 gaattttacc tcggtttcct ttgtatataa tatcattgaa gttatttgca tttctcagaa
    18721 atcttctctg gctttgattg caatagccga aagatagcgc acacatcgcc gttggtcagt
    18781 tagtacgagg tttccgccaa gttcaccttt taccaaagat ggcctcagag cttccctgaa
    18841 gctttatcat ggcttcaagt aggtcatctt cgttcagtga gcgctgagat ttgttgatgc
    18901 taggggcgtg ccgtctgcgg gaaggctcat caccagaaca aggtaacgat tgtgagcgat
    18961 tattgtcccg ttttctctgg acctgacggg caacatccaa cacatggcca agtctcttgt
    19021 tatctacaat ggcgacggga tcaacctcag aaagccggtc ataggtggag tagggcagca
    19081 gcacgccatt tagtcgcagc tccttatgtc cgtcaggata atgccagaca tcaatatact
    19141 tgccgatggc tcttcgactg tactcgttgt cctcaatcag atagagcatt ttatcgtatt
    19201 gtacagtcag attttttgat acccggcgag gctctcgcta ggtaaattct gcatcaaggt
    19261 catcgtctaa tgccagcggc cggtgcgcgt taaaatcatg caagggttct ttggcaaagc
    19321 gccggttata atccttctgc aaatgcattg gccgcttcta ttgtacagat ccccttaagc
    19381 ctgagctcct taacgagtcg gtcctgaagt gtcaaatgag cacgttcaac gcggcctttt
    19441 gcctggctgg tttcagcaca caaaggagta atattaaggc agcgcatggc cctggcaaac
    19501 tgggtttcgc cggggccggt ggtggcgtgc ttattattga ctctgaatac gctggcttta
    19561 tcgctgtaga ggatcatcgg tttaccgtat ttctcgatat agccacgtgt agcttcaaaa
    19621 taggtaaatg tggactcaga tttcacaaac agcaggtgca tcaggcgact ggtggcatcg
    19681 tcaacataga ccagggcaga acagggtcga ccccggtttt caaaccagtg gtggtcgcag
    19741 ccatcaattt gtatcagctc gccagtacaa ggacgccggt aacgaggctg atgaattttc
    19801 ggtgcgcgtt gtctgcgggg aatccagagt ccggccttta tcatgagacg tcggatggtt
    19861 tcctttccaa ggaccagacc gtgaacttcc tccagctttt cccgcgctag cgtggggcca
    19921 aaatcacggt agtgctcccg gattatactg agtgcctgat ctgtcagtga cgctggcaac
    19981 agacggttac cagtgcggcc acgactctgg ttgttcatgc caagcggccc atactggcga
    20041 taacgtttga gcagacggct gcaatgacgt ggcgtgatgc ccagcatttc ggcagcctgt
    20101 ccaggacgga tattgcgatc gatgacatcc tgaaggattt tgaggcgatt gatttctttc
    20161 acggtgaaca ttccggcgtc atttatagcc atagaaaact cctcatcagg aaaaagtgag
    20221 tcgttgtcta gacataggga tctgagaaac tatagcctgg acattcctaa tgagccacaa
    20281 atagacatta gcattgagcc tctacaatgt ttgtgcgcac aatctatctt atgttaaata
    20341 acattgaata ttaacttact tcatgaagct tactgcttct atttccactt ctttttctta
    20401 ttaatcgctc ccctaatcca cgcattcatc tgctctacag gctctaaaat attttttctg
    20461 agtactgagc acaaactgct ccataaaaaa cagaccacaa gtttgggcat gatactgatg
    20521 tagtcacgtt ttattgtttt catgaagctc tctgctattc cattactcac cggactccgc
    20581 accgccgtgt tcttcgattt aagtcccaac atccgagcga actggcgtgt ttcattaacc
    20641 cggtagtcag agtcagcggt cgaaagacct cccaggtagc tactctgcat acccactata
    20701 atgcaactat tctcgtgacg tgtgtttcag ctctcccccg gctatgatac gagatggtat
    20761 ggagagccct tctggggcca ctgcctgcac cagatgcaac aggaagcctg atatgaatgc
    20821 aatttctgtc aggacaggcg ggtaactttc tgtggccatg gatcagggaa tactccaccc
    20881 gacatccacc actgaatttc ttcttcagtc aacaagcaaa gggataaggc atgtgtaaaa
    20941 tcattaactt cagtggcatt gccaatgata gtcaactgac agcagcgatc gccaaatcgc
    21001 ccagaggctg tgtcgatgtg tcgctgtagc gctgaacgtt cttcacgagt taaattattg
    21061 tcagtatgtt ccagtactcc agctttccag tagccaatga gcgccagact gatgcttcct
    21121 gctgactgat tccagagcag ggcaagatca tctctcccgg ggagccaaaa aaaaccttta
    21181 ctgcgataaa cgcccatgcc cataaagcgg tgacaggtat cccataatct ttgcggatgg
    21241 aatggcctgt cgtccctgat gacgcgccat ataagttcct cattgttgca cggagtatcc
    21301 atgtttccta caagtgcttc cagttcactg atgagtaact caacacggtc aaaattgtag
    21361 tccgggatcg ccagtaatgt gctcagttca atatttcccc atgataccgc gagcacatcc
    21421 acggaggggt taagtgggtg tatggctttt gccacagcgg agataatgtc aaagggcagg
    21481 cggtcatttc tggtcagcag cagcctgttg cagaacatga tttgctcggc cagcaaattc
    21541 tccaggccgc gtttgttatg ccggagattt tcctgaagag tggggatcag ttttttccca
    21601 tcattgtaat cttcatggag catgacagta tcaacgagtg ttaaaaacgc cttcaggcgt
    21661 acacgtgaat ggcgacgcag gtatttgacc agaggaagcg gatgactgct tccggaggtt
    21721 tcaatcagca tcacatccgg cttgttgtga tcaagcaggt ggttaattgc gagatccatc
    21781 tttttgaggc ctgcggggct gctgatgctg tctgcggtga tacttacgaa attattgtct
    21841 tttgcatcta caatatctgt gttggcgatc agcacgccgt ccacatccag ttcgctcatg
    21901 tcattgacta tgacagagac tgaaagctgt ttttcgtgtg cctgtatgag caggtttttc
    21961 agcagagtgg ttttccctgc gccgaggaag ccattgagaa tgatgacaga tgttgtttcc
    22021 atatcagcag tacaccagtt agttagcgag gaaggcgctt ggtggtacaa agagcatccg
    22081 gattgaatcg cataacgtcg acgggagttg ttgtcgtttt tcctgtgagg tctcaacctg
    22141 ccacagaacg caccggattt attggtattt agtctccatc agcataatta tcaccatttt
    22201 tatctccggg ggaagatttg ttctgaacag gagtgggagg tgtgacctcc tgaaagtaat
    22261 tacagattgc tagttgttgt atttttattc gaatatgtct cttcgtatca gtgatagcac
    22321 ctagataaag tatattccgg gagcgttatc tgttctagat atcagagagt cagtcatttg
    22381 taaaaaagag agctgcatca ttctattttt cctgtctatt caccctgttg tcgtatctaa
    22441 aagacatccg ttccgaggat gtcgggcacc cgttctgtac taagatacat agcttttggg
    22501 tctgcactat ttggaaccga cagaagtctg tctgtgatat ccgcattttt ctgtgtgcga
    22561 tatattccga aaaacttcaa accacttaaa agaggtgttt ccggaggata agttcgacca
    22621 gggaatagtg tttcctggta agtgtggcgg agccagttca ccaggagtat ctttcttatc
    22681 cagttatact ggattttatt agatgatcac cctaccctgc agcagtacgc tgacggtgtt
    22741 tttttgggag aaccattatg aataacgtca tctttgattg gtatttatct aggcaaaaat
    22801 tcattcaacg tccactgcca agatgcaaca ggcagagtcc ttaactgtca gaatccaagt
    22861 gctcgaaatt attcgaattc cttactaatt cctcctacac aactattgtt atggaagctt
    22921 gcgaagatgc tcactatatg gcacggaaat tttcggaaat gcgtcataaa acaaagctaa
    22981 tacctcctca ttttgtccaa cccttcgtta aaattaataa aaatgatttc tttgaggctg
    23041 aaaccatatg tgaagttaca tcaagccatc aatgagattt gtagcattag gagctgaatc
    23101 ccagcagagg attccacttt tcatccgtcg ttgacaatgg ccttgaaaaa cgccgttact
    23161 caccgttctc cttcacggta atacgcagtt atagttgcag agcaatgtaa tggatctttt
    23221 atattaagaa attgtgtgag tggagaggtg catagatttg cccctatatt tccagacacc
    23281 tgttatcact taacccatta caagcccgct gccgtagata ttcccgtggc gagcgataac
    23341 ccagtgcact atgcggatgc cattcgttat aatgctcgaa cgcctctgca aggttctttg
    23401 ctgccgttaa cccgtctggt ttgggcatga cactgatgta gtcacgcttt atcgttttca
    23461 cgaagctctc tgctatgccg ttactctccg gactccgcac cgccgtgctc ttcggttcaa
    23521 gccccaacat ccgggcaaac tgccgtgttt cattagcccg gtagcatgaa ccattatccg
    23581 tcagccactc cactggagac gccggaagct cgttgccgaa gcggcgttcc accgctccca
    23641 gcatgacgtc ctgtactgtt tcactgttga agccgccggt agtgaccgcc cagtgcagtg
    23701 cctcacgatc acagcagtcc agcgcgaacg tgacacgcag tctctctccg ttatcacagc
    23761 agaactcgaa cccgtcagag caccatcgct gattgctttc tttcacggcc actctgcctg
    23821 tatgtgcccg tttcgatggc ggtacagcag gttttcgctc aagcaacagc gcattctggc
    23881 gcatgatccg gtaaacacgt ttggcattga tcgcaggcat accatcaagt tttgcctgtc
    23941 tgcgaagcag cgcccatacc cgacgataac catacgtggg cagctctctg a
//
LOCUS       DAEVZA010000073        23960 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEVZA010000073 DAEVZA010000000
VERSION     DAEVZA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.073"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            29..2461
                     /gene="bamA"
                     /locus_tag="LAF92_002907"
     CDS             29..2461
                     /gene="bamA"
                     /locus_tag="LAF92_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBI9791879.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            2583..3068
                     /gene="skp"
                     /locus_tag="LAF92_002908"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             2583..3068
                     /gene="skp"
                     /locus_tag="LAF92_002908"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBI9791880.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            3072..4097
                     /gene="lpxD"
                     /locus_tag="LAF92_002909"
     CDS             3072..4097
                     /gene="lpxD"
                     /locus_tag="LAF92_002909"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBI9791881.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            4202..4657
                     /gene="fabZ"
                     /locus_tag="LAF92_002910"
     CDS             4202..4657
                     /gene="fabZ"
                     /locus_tag="LAF92_002910"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBI9791882.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            4661..5449
                     /gene="lpxA"
                     /locus_tag="LAF92_002911"
     CDS             4661..5449
                     /gene="lpxA"
                     /locus_tag="LAF92_002911"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBI9791883.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            5449..6597
                     /gene="lpxB"
                     /locus_tag="LAF92_002912"
     CDS             5449..6597
                     /gene="lpxB"
                     /locus_tag="LAF92_002912"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBI9791884.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            6594..7190
                     /gene="rnhB"
                     /locus_tag="LAF92_002913"
     CDS             6594..7190
                     /gene="rnhB"
                     /locus_tag="LAF92_002913"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBI9791885.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            7227..10709
                     /gene="dnaE"
                     /locus_tag="LAF92_002914"
     CDS             7227..10709
                     /gene="dnaE"
                     /locus_tag="LAF92_002914"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBI9791886.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            10722..11681
                     /gene="accA"
                     /locus_tag="LAF92_002915"
     CDS             10722..11681
                     /gene="accA"
                     /locus_tag="LAF92_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBI9791887.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            11780..13921
                     /gene="ldcC"
                     /locus_tag="LAF92_002916"
     CDS             11780..13921
                     /gene="ldcC"
                     /locus_tag="LAF92_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBI9791888.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            13978..14367
                     /locus_tag="LAF92_002917"
     CDS             13978..14367
                     /locus_tag="LAF92_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI9791889.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            14432..15730
                     /gene="tilS"
                     /locus_tag="LAF92_002918"
     CDS             14432..15730
                     /gene="tilS"
                     /locus_tag="LAF92_002918"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBI9791890.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(15779..16039)
                     /gene="rof"
                     /locus_tag="LAF92_002919"
     CDS             complement(15779..16039)
                     /gene="rof"
                     /locus_tag="LAF92_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBI9791891.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(16026..16226)
                     /locus_tag="LAF92_002920"
     CDS             complement(16026..16226)
                     /locus_tag="LAF92_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBI9791892.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            16392..16937
                     /locus_tag="LAF92_002921"
     CDS             16392..16937
                     /locus_tag="LAF92_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBI9791893.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            16934..17356
                     /gene="arfB"
                     /locus_tag="LAF92_002922"
     CDS             16934..17356
                     /gene="arfB"
                     /locus_tag="LAF92_002922"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBI9791894.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            17370..18080
                     /gene="nlpE"
                     /locus_tag="LAF92_002923"
                     /gene_synonym="cutF"
     CDS             17370..18080
                     /gene="nlpE"
                     /locus_tag="LAF92_002923"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBI9791895.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(18235..19058)
                     /locus_tag="LAF92_002924"
                     /pseudo
     CDS             complement(18235..19058)
                     /locus_tag="LAF92_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(19112..20830)
                     /gene="proS"
                     /locus_tag="LAF92_002925"
     CDS             complement(19112..20830)
                     /gene="proS"
                     /locus_tag="LAF92_002925"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBI9791896.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(20942..21649)
                     /gene="tsaA"
                     /locus_tag="LAF92_002926"
     CDS             complement(20942..21649)
                     /gene="tsaA"
                     /locus_tag="LAF92_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBI9791897.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(21646..22050)
                     /gene="rcsF"
                     /locus_tag="LAF92_002927"
     CDS             complement(21646..22050)
                     /gene="rcsF"
                     /locus_tag="LAF92_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBI9791898.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(22168..22983)
                     /gene="metQ"
                     /locus_tag="LAF92_002928"
     CDS             complement(22168..22983)
                     /gene="metQ"
                     /locus_tag="LAF92_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBI9791899.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(23023..23676)
                     /gene="metI"
                     /locus_tag="LAF92_002929"
     CDS             complement(23023..23676)
                     /gene="metI"
                     /locus_tag="LAF92_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBI9791900.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(23669..>23960)
                     /gene="metN"
                     /locus_tag="LAF92_002930"
     CDS             complement(23669..>23960)
                     /gene="metN"
                     /locus_tag="LAF92_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBI9791901.1"
                     /translation="DIPEDYQERLQAEPFTDCVPMLRLEFTGQSVDAPLLSETARRFN
                     VNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHHVKVEVLGYV"
BASE COUNT         5772 a         6013 c         6583 g         5592 t
ORIGIN      
        1 agttagttag gaagaacgca taataacgat ggcgatgaaa aagttgctca tagcgtcgct
       61 gctgtttagc agcgccaccg tatacggtgc tgaagggttc gtagtgaaag atattcattt
      121 cgaaggcctt cagcgtgtcg ccgttggtgc ggccctcctc agtatgccgg tgcgcacagg
      181 cgacacggtt aatgatgaag atatcagtaa taccattcgc gctctgtttg ctaccggcaa
      241 ctttgaggat gttcgcgtcc ttcgtgatgg tgataccctt ctggttcagg taaaagaacg
      301 tccgaccatt gccagcatta ctttctccgg taacaaatcg gtgaaagatg acatgctgaa
      361 gcaaaacctc gaggcttctg gtgtgcgtgt gggcgaatcc ctcgatcgca ccaccattgc
      421 cgatatcgag aaaggtctgg aagacttcta ctacagcgtc ggtaaatata gcgccagcgt
      481 aaaagctgtc gtgaccccgc tgccgcgcaa ccgtgttgac ctaaaactgg tgttccagga
      541 aggtgtgtca gctgaaatcc agcaaattaa cattgttggt aaccatgctt tcaccaccga
      601 cgaactgatc tctcatttcc aactgcgtga cgaagtgccg tggtggaacg tggtaggcga
      661 tcgtaaatac cagaaacaga aactggcggg cgaccttgaa accctgcgca gctactatct
      721 ggatcgcggt tatgcccgtt tcaacatcga ctctacccag gtcagtctga cgccagataa
      781 aaaaggtatt tacgtcacgg tgaacattac cgaaggcgat cagtacaagc tttctggcgt
      841 tgaagtgagc ggcaaccttg ccgggcactc cgctgaaatt gagcagctga ctaagatcga
      901 gccgggcgag ctgtataacg gcaccaaagt gaccaagatg gaagatgaca tcaaaaagct
      961 tctcggtcgc tatggttatg cctatccgcg cgtacagtcg atgcctgaaa ttaacgatgc
     1021 cgacaaaacc gttaaattac gcgtgaacgt tgatgcgggt aaccgtttct acgtgcgtaa
     1081 gatccgtttt gaaggtaacg atacctcgaa agatgccgtc ctgcgtcgcg aaatgcgtca
     1141 gatggaaggt gcatggctgg gaagcgatct ggtcgatcag ggtaaggagc gtctgaatcg
     1201 tctgggcttc tttgaaactg tcgataccga tacccaacgt gttccgggta gcccggacca
     1261 ggtagatgta gtctacaagg tgaaagaacg taacaccggt agcttcaact tcgggattgg
     1321 ttacggtact gaaagtggcg tgagcttcca ggctggtgtg cagcaggata actggttagg
     1381 tacaggttat gctgttggta tcaacgggac caaaaacgat taccagacct atgctgaact
     1441 gtcggtaacc aacccgtact tcactgtaga tggtgtaagc ctcggtggtc gtctcttcta
     1501 taatgacttc caggcagatg acgccgacct gtccgactat accaacaaga gttatggtac
     1561 agacgtgacg ttgggcttcc cgatcaacga atataactcg ctgcgtgcag gtctgggtta
     1621 tgtacataac tccctgtcca acatgcagcc tcaggttgcg atgtggcgtt atctgtactc
     1681 tatgggtgaa catccgagca cctctgatca ggataatagc ttcaaaacgg acgacttcac
     1741 gttcaactat ggttggacct ataacaagct tgaccgtggt tacttcccga cagatggttc
     1801 acgtgtcaac ctgaccggta aagtgaccat tcctggatcg gataacgaat actacaaagt
     1861 gacgttagac acggcgactt atgtgccgat cgatgacgat cacaaatggg ttgttctggg
     1921 gcgtacccgc tggggttatg gtgatggttt aggcggcaaa gagatgccgt tctacgagaa
     1981 cttctatgcc ggtggttcca gcaccgtgcg tggcttccag tccaatacaa ttggtccgaa
     2041 agcagtttac ttcccgcatc aggccagtaa ttatgatccg gactatgatt acgaatgtgc
     2101 gactcaggac ggcgcgaaag acctgtgtaa atcggatgat gctgtaggcg gtaacgccat
     2161 ggcggttgcc agcctcgagt tcatcacccc gacgccgttt attagcgata agtatgctaa
     2221 ctcggttcgt acttccttct tctgggatat gggtaccgtt tgggatacaa actgggattc
     2281 cagccaatat tctggatatc cggactatag tgatccaagc aatatccgta tgtctgcggg
     2341 tatcgcatta caatggatgt ccccattggg gccgttggtg ttctcctacg cccagccgtt
     2401 caaaaagtac gatggagaca aggcagaaca gttccagttt aacatcggta aaacctggta
     2461 agtgttctcc acaaaggaat gtagtggtag tgtagcgatg actttaggcg atcaatataa
     2521 gatcgccggg ccacgcaaag aactgcaccc tccggtgcaa atgggatggt aaggagttta
     2581 ttgtgaaaaa gtggttatta gctgcaggtc tcggtttagc actggcaact tctgctcagg
     2641 cggctgacaa aattgcaatc gtcaacatgg gcagcctgtt ccagcaggta gcgcagaaaa
     2701 ccggtgtttc taacacgctg gaaaatgagt tcaaaggccg tgccagcgaa ctgcagcgta
     2761 tggaaaccga tctgcaggct aaaatgaaaa agctgcagtc catgaaagcg ggcagcgatc
     2821 gcactaagct ggaaaaagac gtgatggctc agcgccagac ttttgctcag aaagcgcagg
     2881 cttttgagca ggatcgcgca cgtcgttcca acgaagaacg cggcaaactg gttactcgta
     2941 tccagactgc tgtgaaatcc gttgccaaca gccaggatat cgatctggtt gttgatgcaa
     3001 acgccgttgc ttacaacagc agcgatgtaa aagacatcac tgccgacgta ctgaaacagg
     3061 ttaaataagt aatgccttca attcgactgg ctgatttagc gcagcagttg gatgcagaac
     3121 tacacggtga tggcgatatc gtcatcaccg gcgttgcgtc catgcaatct gcacaaacag
     3181 gtcacattac gttcatggtt aacccaaaat accgtgagca tttaggcttg tgccaggcgt
     3241 ccgcggttgt catgacccag gacgatcttc ctttcgcgaa aagtgccgcg ctggtagtga
     3301 agaatcccta cctgacttac gcgcgcatgg cgcaaatttt agataccacg ccgcagcccg
     3361 cgcagaacat tgcacccagt gcggtgatcg acgcgacggc gaagctgggt aacaacgtat
     3421 cgattggcgc taacgcggtg attgagtccg gcgttgaact gggcgataac gtgattatcg
     3481 gtgccggttg cttcgtaggt aaaaacagca aaatcggtgc aggttcgcgt ctctgggcga
     3541 acgtaaccat ttaccatgag atccagatcg gtcagaattg cctgatccag tccggaacag
     3601 tggtaggcgc agacggcttt ggttatgcca acgatcgtgg taactgggtg aagatcccac
     3661 agattggtcg cgtaattatt ggcgatcgcg tggagatcgg tgcctgcaca accatcgatc
     3721 gcggcgcgct ggatgacact attattggca atggcgtgat cattgataac cagtgccaga
     3781 ttgcacataa cgtcgtgatt ggcgacaata cggcggttgc cggtggcgtc attatggcgg
     3841 gcagcctgaa aattggtcgt tactgcatga tcggcggagc cagcgtaatc aacgggcata
     3901 tggaaatatg cgacaaagtg acggttacgg gcatgggtat ggtgatgcgt cccatcactg
     3961 aaccaggcgt ctattcctca ggcattccgc tgcaacccaa caaagtctgg cgcaaaaccg
     4021 ctgcactggt gatgaacatt gatgacatga gcaagcgtct gaaatcgctt gagcgcaagg
     4081 ttaatcaaca agactaacgt tccatctttt gttcgccaaa ctttacggcc tgtctcattc
     4141 ttacgattgc ggcaggccgt gttattattg tcgtttctta tattttgaca ggaagagtat
     4201 cttgactact aacactcata ctctgcagat tgaagagatt ttagaacttc tgccgcaccg
     4261 tttcccgttc ttactggtgg atcgcgtgct ggattttgaa gaaggtcgtt ttctgcgcgc
     4321 agtaaaaaat gtctctgtca atgagccatt cttccagggc catttccctg gaaaaccgat
     4381 tttcccgggt gtgctgattc tggaagcaat ggcacaggca acaggtattc tggcgtttaa
     4441 aagcgtagga aaactggaac cgggtgaact gtactacttc gctggtattg acgaagcgcg
     4501 cttcaagcgc ccggtcgtgc ctggcgatca aatgatcatg gaagtcactt tcgaaaaaac
     4561 gcgccgcggc ctgacccgtt ttaaaggggt tgctctggtc gatggtaaag tagtttgcga
     4621 agcaacgatg atgtgtgctc gtagccggga ggcctgataa gtgattgata aatccgcctt
     4681 tgtgcatcca accgccattg tggaagaggg cgcgtcaatt ggcgcgaacg cacacattgg
     4741 tcctttttgt atcgttggac cccatgtcga aattggtgag ggtaccgtac tgaaatctca
     4801 cgttgtcgtg aatggtcata ctaaaattgg ccgcgataat gagatttatc agttcgcctc
     4861 catcggcgaa gttaaccagg atctgaaata tgctggcgaa ccgacccgtg tggaaatcgg
     4921 cgatcgtaac cgcattcgcg aaagcgtcac cattcatcgt ggcacagtcc agggcggtgg
     4981 attgacgaag gtgggcagcg acaacttact gatgatcaac gcgcacattg cgcacgattg
     5041 tacggtaggt aaccgctgta ttctcgccaa caacgcaacg ctggcgggtc acgtatcggt
     5101 tgacgacttc gcgatcatcg gcggcatgac cgcagtccat cagttctgca tcattggtgc
     5161 gcacgtgatg gttggcggct gctccggtgt ggcgcaggac gtccctcctt atgtcattgc
     5221 gcagggtaac cacgcaacgc cgttcggtgt caatatcgaa gggctgaagc gccgcggatt
     5281 cagccgtgag gcgattaccg ctatccgcaa tgcgtataag ctgatttatc gtagcggtaa
     5341 aacgctcgat gaagtgaaac cggaaattgc tgaactggcg gaaacatatc cggaagtgaa
     5401 agcctttacc gatttctttg cacgctcaac gcgcggtctg attcgttaat gactgaacag
     5461 cgtccattaa cgattgccct ggtcgccgga gaaacctccg gcgatatcct gggggccggt
     5521 ttaatccgcg ctctgaaaga acgtgtgccc aacgcccgct ttgttggtgt tgccgggcca
     5581 cgaatgcagg ctgaaggctg cgaagcctgg tacgaaatgg aagaactggc ggtaatgggc
     5641 attgttgaag tgctcggtcg tctgcgtcgc ttactgcata ttcgtgccga tctgacaaag
     5701 cgttttggcg aactgaagcc agatattttt gttggtattg atgcgcctga cttcaatatt
     5761 actcttgaag gtaacctcaa aaagcagggt atcaaaacca ttcattacgt cagtccatcc
     5821 gtctgggcgt ggcgacagaa acgcgttttc aaaataggca gagccaccga tctggtgctc
     5881 gcatttctgc ctttcgaaaa agcgttttat gacaaataca atgtaccgtg ccgctttatc
     5941 ggtcatacca tggctgatgc catgccgtta gatccagata aaaatgccgc ccgtgatgtg
     6001 ctggggatcc ctcacgatgc ccactgcctg gcgttgctac cagggagccg tggtgcagaa
     6061 gttgaaatgc ttagtgccga tttcctgaaa acggcccagc ttttgcgcca gacatatccg
     6121 gatctcgaaa tcgtggtgcc actggtgaat gccaaacgcc gcgagcagtt tgaacgcatc
     6181 aaagctgaag tcgcgccaga cctttcagtt catttgctgg atgggatggg ccgtgaggcg
     6241 atggtcgcca gcgatgcggc gctactggcg tcgggtacgg cagccctgga gtgtatgctg
     6301 gcgaaatgcc cgatggtggt gggatatcgc atgaagcctt ttaccttctg gttggcgaag
     6361 cggctggtga aaactgatta tgtctcgctg ccaaatctgc tggcgggtag agagttagtc
     6421 aaagaattat tgcaggaaga gtgtgagccg caaaaactgg ctgcggcgct gttaccgctg
     6481 ttggcgaacg ggaaaaccag ccacgcgatg cacgatacct tccgtgaact gcatcagcag
     6541 atccgctgca atgccgatga gcaggcggca caagccgttc tggagttagc acaatgatcg
     6601 aatttgttta tccgcacacg cagctggttg cgggtgtgga tgaagtcgga cgcgggccgt
     6661 tagttggcgc ggtcgtcacc gctgcggtga tccttgaccc ggcgcgcccg attgccgggc
     6721 tgaatgattc caaaaagctg agcgaaaaac gccgtctggc gctctatgaa gagatcaaag
     6781 agaaagcgtt gagctggagt ctgggccgcg cggaacccca cgaaatcgac gagctgaaca
     6841 ttcttcatgc gaccatgctg gcgatgcagc gtgccgtcgc tgggctgcat attgcgccgg
     6901 aatatgtgtt gattgatggt aaccgctgcc cgaaattacc gatgcctgcg atggctgtgg
     6961 tgaaaggcga tagccgcgta ccggaaatca gtgccgcgtc tatcctggcg aaagtgacgc
     7021 gtgacgccga aatggcggcg ctggatattg ttttcccgca atatggtttt gcccaacaca
     7081 aagggtaccc aaccgctttt catctggaaa aactggctga acacggcgcg accgaacacc
     7141 atcggcgcag ctttgggcct gtcaaacgcg cactgggact tgcgtcctga ttcttgtgtc
     7201 gagattaagt aaaccggaat ctgaagatgt ctgaaccacg tttcgtacac ctgcgggtgc
     7261 acagcgacta ctcgatgatc gatggcctgg ccaaaaccgc accgctggta aaaaaggcgg
     7321 cggcgttggg tatgccagca ctggcgatca ccgatttcac caacctttgc ggtctggtga
     7381 agttctacgg agcgggacat ggcgcaggga ttaagcctat cgtcggggca gattttaacg
     7441 tccagtgcga cctgctgggt gatgagttaa cccacctgac ggtactggcg gcgaacaata
     7501 ccggctatca gaatctgacg ttgctgatct caaaagcgta tcagcgcggg tacggtgccg
     7561 ccgggccgat catcgatcgc gactggctta tcgaattaaa cgaagggttg atccttcttt
     7621 ccggcggacg catgggcgac gtcggacgca gtcttttgcg tggtaacagc gcgctggtag
     7681 atgagtgtgt cgcgttttat gaagaacact tcccggatcg ctattttctc gagctgatcc
     7741 gcaccggcag gccggacgaa gaaagctatc tgcacgcggc ggtggaactg gcggaagcgc
     7801 gcggtttgcc cgtcgtggcg accaacgacg tgcgctttat cgacagcagc gactttgacg
     7861 cacacgaaat ccgcgtcgcg atccacgacg gctttaccct cgacgatcct aaacgcccgc
     7921 gtaactattc gccgcagcaa tatatgcgta gcgaagagga gatgtgtgag ctgtttgccg
     7981 acatccccga agcccttgcc aacaccgttg agatcgccaa acgctgtaac gtaaccgtgc
     8041 gtcttggtga atacttcctg ccgcagttcc cgaccgggga catgagcacc gaagattatc
     8101 tggtcaagcg tgcaaaagag ggcctggaag agcgtctggc ctttttattc cctgatgagg
     8161 aagaacgtgt taagcgccgc ccggaatatg acgaacgtct ggagactgaa cttcaggtta
     8221 tcaaccagat gggcttcccg ggctacttcc tcatcgttat ggaatttatc cagtggtcga
     8281 aagataacgg cgtaccggta gggccaggcc gtggctccgg tgcgggttca ctggtggcct
     8341 acgcgctgaa aatcaccgac ctcgatccgc tggaatttga cctgctgttc gaacgtttcc
     8401 ttaacccgga acgtgtctcc atgcctgact tcgacgttga cttctgtatg gagaaacgcg
     8461 atcaggttat cgaacacgtg gcggacatgt acggtcgtga tgcggtatcg cagatcatca
     8521 ccttcggtac aatggcggcg aaagcggtga tccgcgacgt aggccgcgtg ctggggcatc
     8581 cgtacggctt tgtcgatcgt atctcgaaac tgatcccgcc cgatccgggg atgacgctgg
     8641 cgaaagcgtt tgaagccgag ccgcagctgc cggaaatcta cgaagcggat gaagaagtta
     8701 aggcgctgat cgacatggcg cgcaaactgg aaggggtcac ccgtaacgcc ggtaagcacg
     8761 ccggtggggt ggttatcgcg ccgaccaaaa ttaccgattt tgcgccgctt tactgcgatg
     8821 aagagggcaa acatccggtc acccagtttg ataaaagcga cgttgaatac gccggactgg
     8881 tgaagttcga cttccttggt ttgcgtacgc tcaccatcat caactgggcg ctggagatga
     8941 tcaacaagcg gcgggcgaag aatggcgagc cgccgctgga tatcgccgcg atcccgctgg
     9001 atgacaagaa aagcttcgac atgctgcaac gctcggaaac cacggcggta ttccagcttg
     9061 aatcgcgcgg catgaaggac ctgatcaagc gtctgcaacc tgactgcttc gaagatatga
     9121 tcgcactggt ggcgctgttc cgcccaggtc cgttgcaatc agggatggtg gataacttta
     9181 tcgaccgtaa acatggtcgc gaagaaatct cctatccgga cgtacagtgg cagcatgaaa
     9241 gcctgaaacc ggtactggag ccaacctacg gcatcatcct gtatcaggaa caggtcatgc
     9301 agattgcgca ggtgctttct ggttataccc tcggtggcgc ggatatgctg cgtcgtgcga
     9361 tgggtaagaa aaagccggaa gagatggcta agcagcgttc tgtatttgct gaaggtgcag
     9421 aaaagaacgg aatcaacgcc gaactggcga tgaaaatctt cgacctggtg gagaaattcg
     9481 cgggttacgg atttaacaaa tcgcactctg cggcctatgc tttggtgtca tatcaaacgt
     9541 tatggctgaa agcgcactat ccggcggagt ttatggcggc ggtaatgacc gccgatatgg
     9601 acaacaccga gaaggtggtg ggcctggtgg atgagtgctg gcggatgggg ctgaaaatcc
     9661 tgccaccaga tataaactcc ggtctttacc atttccacgt caacgacgac ggcgaaatcg
     9721 tgtatggtat tggcgccatc aaaggggtag gtgaaggtcc gattgaggcc atcatcgaag
     9781 cccgtaataa aggcggctac ttccgcgaac tgtttgatct ctgcgcccgt accgacacca
     9841 aaaagttaaa ccggcgggtg ctggaaaaac tgatcatgtc cggggcgttt gaccgtcttg
     9901 ggccacaccg cgcggcgctg atgaactcgc tgggtgatgc gttaaaagcg gcagatcaac
     9961 acgcgaaagc ggaagctatc ggtcaggccg atatgttcgg cgtgctggcc gaagagccgg
    10021 aacaaattga acaatcctac gccagctgcc aaccgtggcc ggagcaggtg gtattagatg
    10081 gggaacgtga aacgttaggc ctgtacctga ccggacaccc tatcaaccag tatttaaaag
    10141 agattgagcg ttatgtcgga ggcgtaaggc tgaaagacat gcacccgaca gaacgtggta
    10201 aagtcatcac ggctgcgggg ctcgttgttg ccgcgcgggt tatggtcacc aagcgcggca
    10261 atcgtatcgg tatctgcacg ctggatgacc gttccgggcg gctggaagtg atgttgttta
    10321 ctgacgccct ggataaatac cagcaattgc tggaaaaaga ccgcatactt atcgtcagcg
    10381 gacaggtcag ctttgatgac ttcagcggtg ggcttaaaat gaccgctcgc gaagtgatgg
    10441 atattgacga agcccgggaa aaatatgctc gcgggcttgc tatctcgctg acggacaggc
    10501 aaattgatga ccagctttta aaccgactcc gtcagtctct ggaaccccac cgctctggga
    10561 caattccagt acatctctac tatcagaggg cggatgcacg cgcgcggttg cgttttggcg
    10621 cgacgtggcg tgtctctccg agcgatcgtt tattaaacga tctccgtggc ctcattggtt
    10681 cggagcaggt ggaactggag tttgactaat acaggaatac tatgagtctg aatttccttg
    10741 attttgaaca gccgattgca gagctggaag cgaaaatcga ttctctgact gcggttagcc
    10801 gtcaggatga gaaactggat attaacatcg atgaagaagt gcatcgtctg cgtgaaaaaa
    10861 gcgtagaact gacgcgtaaa atcttcgccg atcttggtgc atggcagatt gcgcaactgg
    10921 cacgccatcc acagcgtcct tataccctgg attacgttcg cctggcattt gatgaatttg
    10981 acgaactggc tggcgaccgc gcgtatgcag acgataaagc tatcgtcggt ggtatcgccc
    11041 gtctcgatgg tcgtccggtg atgatcattg gtcatcaaaa aggtcgtgaa accaaagaaa
    11101 aaattcgccg taactttggt atgccagcgc cagaaggtta ccgcaaagca ctgcgtctga
    11161 tgcaaatggc tgaacgcttt aagatgccaa tcatcacctt tatcgacacc ccgggggctt
    11221 atccgggcgt gggcgcagaa gaacgtggtc agtctgaagc cattgcacgc aacctgcgtg
    11281 aaatgtctcg cctcggcgta ccggtagttt gtacggttat cggtgaaggt ggttctggcg
    11341 gtgcgctggc gattggcgtg ggcgataaag tgaatatgct gcaatacagc acctattccg
    11401 ttatctcgcc ggaaggttgt gcgtccattc tgtggaagag cgccgacaaa gcgccgctgg
    11461 cggctgaagc gatgggtatc atcgctccgc gtctgaaaga gctgaaactg atcgactcca
    11521 tcatcccgga accgctgggt ggcgctcacc gtaacccgga agcgatggcg gcatcgttga
    11581 aagcgcaact gctggcggat ctggccgatc tcgatgtgtt aagcactgaa gatttaaaaa
    11641 atcgtcgtta tcagcgcctg atgagctacg gttacgcgta attcgcaaaa gttctgaaaa
    11701 agggtcactt cggtggccct tttttatcgc cacggtttga gcaggctatg attaaggaag
    11761 gattttccag gaggaacaca tgaacatcat tgccattatg ggaccgcatg gcgtctttta
    11821 taaagatgag cccatcaaag aactggagtc ggcgctggtg gcgcaaggct ttcagattat
    11881 ctggccacaa aacagcgttg atttgctgaa atttatcgaa cataaccctc gaatttgcgg
    11941 cgtgattttt gactgggatg agtacagtct cgatttatgc agcgatatca atcaacttaa
    12001 tgaatatctc ccgctttatg ccttcatcaa cacccactcg acgatggatg tcagcgtgca
    12061 ggatatgcgg atggcgctct ggttttttga atatgcgctg gggcaggcgg aagatatcgc
    12121 cattcgtatg cgtcagtaca ccgacgaata tcttgataac attacaccgc cgttcacgaa
    12181 agccttgttt acctacgtta aagagcggaa gtacaccttt tgtacgccgg ggcatatggg
    12241 cggcaccgca tatcaaaaaa gcccggttgg ctgcctgttt tatgattttt tcggcgggaa
    12301 taccctcaag gctgatgtct ctatttcggt caccgagctt ggttcgttgc tcgaccacac
    12361 cgggccacac ctggaagcgg aagagtacat cgcgcggact tttggcgcgg aacagagtta
    12421 tatcgttacc aacggaacat cgacgtcgaa caaaattgtg ggtatgtacg ccgcgccatc
    12481 cggcagtacg ctgttgatcg accgcaattg tcataaatcg ctggcgcatc tgttgatgat
    12541 gaacgatgta gtgccagtct ggctgaaacc gacgcgtaat gcgttgggga ttcttggtgg
    12601 gatcccgcgc cgtgaattta ctcgcgacag catcgaagag aaagtcgctg ccaccacgca
    12661 ggcacaatgg ccggttcatg cggtgattac caactccacc tatgatggct tgctctacaa
    12721 caccgactgg atcaaacaga cgctggatgt cccgtcgatc cacttcgatt ccgcctgggt
    12781 gccgtacacc cattttcatc cgatctacca ggggaaaagt ggtatgagcg gcgagcgtgt
    12841 tgcgggaaaa gtgatcttcg aaacgcaatc gacccacaaa atgctggcgg cgttatcgca
    12901 ggcttcgctg atccacatta aaggcgagta tgacgaagag gcgtttaacg aagcctttat
    12961 gatgcatacc accacctcgc ccagttatcc cattgttgct tcggttgaga cggcggcggc
    13021 gatgctgcgt ggtaatccgg gcaaacggct gattaaccgt tcggtggagc gggcgctgca
    13081 ttttcgcaaa gaggtccagc ggttgcggga agagtctgac ggttggtttt tcgatatctg
    13141 gcaaccgccg caggtggatg aagccgaatg ctggcccgtt gcgccaggcg aacagtggca
    13201 cggctttaac gatgcggatg ccgatcatat gtttctcgat ccggttaaag tcactatttt
    13261 gacaccgggg atggacgaac agggcaatat gagtgaggag gggatcccgg cggcgctggt
    13321 ggcaaaattc ctcgacgaac gtgggatcgt agtagagaaa accggccctt ataacctgct
    13381 gtttctcttt agtattggta tcgataaaac caaagcaatg ggattattgc gtgggttgac
    13441 ggaatttaaa cgctcttacg atctcaacct gcggatcaaa aatatgctac ccgatctcta
    13501 tgctgaagat cccgatttct accgcaatat gcgtattcag gatctggcgc aagggatcca
    13561 taagctgatt cgtaaacacg atcttcccgg tttgatgttg cgggcattcg atactttgcc
    13621 ggaaatgatc atgacgccac atcaggcatg gcagagacag attaaaggtg aagtagaaac
    13681 cattgcgctg gaacaactgg tcggtagagt atcggcaaat atgatcctgc cttatccacc
    13741 gggcgtaccg ctgttgatgc caggagaaat gctgaccaaa gagagccgca cggtactcga
    13801 ttttctactg atgctttgtt ccgtagggca acattacccc ggttttgaaa cggatattca
    13861 cggcgcgaaa caggacgaag acggcgttta ccgcgtacga gtcctaaaaa tggcgggata
    13921 acttgccaga gcggcttccg ggcgagtaac gtgctgttaa caaataaagg agacgttatg
    13981 ctgggtttaa aacaggttca ccatattgcg attattgcga cggattatgc ggtgagcaaa
    14041 gctttctact gcgatattct tggtttcacg ctgcaaagcg aagtctatcg cgaagcgcgc
    14101 gactcgtgga aaggggattt ggcgcttaat gggcaatatg tgattgagct tttctctttt
    14161 ccgttcccgc cggagcgccc cagccgaccg gaagcttgcg gcctgcgtca tctggctttt
    14221 agcgttgatg acatcgatgc ggcagtggcg caccttgaaa gccataacgt gaagtgtgaa
    14281 gccatccgtg tcgatccata cacgcaaaaa cgcttcacct tctttaacga tccggacggg
    14341 ctgccgttgg aactgtatga gcagtaaggc ttgtcatcgc cgcatttgcc cggtaacgtg
    14401 ccgggcattg ctactgtaaa atcgcaccat catgacactc acgctcaata gacaacttct
    14461 cacctcacgc cagattctgg tggcctttag cggcgggctt gactccaccg ttctgctgca
    14521 tcagttggtg cagtggcgga cggaaaatcc gggcgtcact ctgcgcgcta ttcatgtgca
    14581 tcacggttta agtgccaatg ccgatgcctg ggttacgcat tgcgaaaacg tctgccaaca
    14641 gtggcaggtg ccgctggtgg tcgaacgcgt acaacttgcg caagaggggc tggggattga
    14701 ggcccaagcg cggcaggcgc gttatcaggc atttgcccgt accctattgc ccggcgaagt
    14761 gctggtcacc gcgcaacatc tcgacgatca atgtgaaacc tttctgctgg cgctaaaacg
    14821 cggtagcggc cctgccgggc tttcggctat gggggaagtc tcggagtttg ccggaacacg
    14881 gcttatccgc ccgttgcttg cccgcacgcg gggggaactg gcgcagtggg cattggcgca
    14941 tggtttacgc tggattgaag acgaaagtaa tcaggacgac tcatacgatc gtaactttct
    15001 gcgcctgcgt gtagtgccgt tattgcagca gcgttggccg cattttgccg aagcaacggc
    15061 ccgcagcgcc gcactttgtg ctgaacaaga gagtctgttg gatgaactgc tggcggatga
    15121 tttagcacac tgccaaacgt cgcaggggac gctgcagatt gcgccaatgc tagcgatgag
    15181 tgatgcccgt cgcgcggcga ttatccgccg ctggctggca gggcagaatg caccgatgcc
    15241 ttcccgcgac gcgctggtgc ggatctggca ggaagtggcg ctggcgcggg aagatgcctc
    15301 cccctgttta cgcttgggcg cgtttgaaat ccgccgatat cagtcgcaac tgtggtggat
    15361 taaatccgtc accgggcaaa gtgaaaccat tgtgccgtgg cagacgtggc tacaaccgct
    15421 ggaattaccg gcggggctgg gaagcgtaca gcttactgcg ggaggcgata ttcgccctcc
    15481 gcgtgcagac gaagcggtca gcgtgcgttt caaagcggca ggattgctgc atattgtcgg
    15541 gcgcaacggc ggacgtaagc tgaagaaaat ctggcaagag ttaggcgtgc cgccgtggct
    15601 acgtgacacc acgccactgc tgttttatgg cgaaacgctg attgcggcgg caggggtatt
    15661 tgtgacgcaa gaaggtgtgg ctgaaggtga gaatggcgta agttttgtct ggcagaaaac
    15721 gcttagttaa gtgaaagccg gataagacgc atcaaacgtc gcatccggcg aaagtcaatc
    15781 aggactcgct caccaccacc gtaccgattt ccgggtggct aaagctggta attttatcca
    15841 gacgtagctc acgggtttcg ccagcggcct cgacgaccag atactccaca tttttgcggg
    15901 agactaaatc gctggctttt gcctgcaatt tttcgccatc tttcagctca agtgtcagca
    15961 ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc atcacaattg attggttgat
    16021 acgtatcatt cattgacata atcgctcacc agtaagtttg ccgcagcgta tgctgctttt
    16081 tccctgacag cctcagaaag ggcgtcgtcg gcagccattt cattcagcac tttcaaaacg
    16141 cagcccagcg cgtccggaac gtatcctaag tctccgctgg cgatttccgc gtaccgcttg
    16201 cgtattaact cacaatattt ttccacatgc cctcctgtca gcactctgac ttaaccgtgg
    16261 atgcaagtct aagcctacga agataaactc tgtttcgcaa ggtgactata ccacactcat
    16321 ttctgcaata tcagcgccgc aactgcacgt attccgttac aatggcctcc tgattcgaaa
    16381 ggagttttct tatggcgctt aaagcgacaa tttataaagc gacggttaat gtggccgatc
    16441 tcgaccgcaa ccagtttctc gatgcctctc tgacgctggc gcgccaccct tcagaaaccc
    16501 aggagcgtat gatgctgcgc ttgctggcgt ggctgaaata tgctgatgaa cgtctgcaat
    16561 ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg gctgcgtaac gatcacctgg
    16621 gcattgattt gtggattgag ctggggctgc cggatgagcg gcggattaag aaagcctgca
    16681 cccaggccgc agaagtggcg ctgtttacct ataatagtcg ggcggcgcaa atctggtggc
    16741 agcaaaatca gagcaaatgt gtgcagtttg ccaatctttc cgtctggtat ctggacgatg
    16801 aacaactggc gaaagtaagc gcctttgccg atcgtaccat gactctgcag gcaacgattc
    16861 aggatggcgt gatctggtta tcggacgata agaataatct ggaagttaac ttaaccgcct
    16921 ggcaacaacc ttcatgattg tgatttcccg acatgttgct attcccgatg gtgagcttga
    16981 gatcaccgcc attcgtgcgc agggcgcggg cgggcagcat gttaataaga cctcaacggc
    17041 cattcatctg cgttttgaca ttcgggcgtc cagcctgcca gagtattaca aagagcgtct
    17101 actcgccgcc agccatcatt tgatcagcag tgatggcgtg attgtcatta aggcacagga
    17161 ataccgcagt caggaactga accgcgaagc cgcgctagcc cggctggtgg cagtgattaa
    17221 agatttaaca acagaacaaa aagcccgacg acccacgcgg cccacccgtg catcgaaaga
    17281 gcgcaggctg gcatcgaaag cacaaaaatc aagcgtgaag gcgatgcgcg gcaaagtacg
    17341 cagcggtcgg gaataaaaag aaggaataga tggtgaaaaa agcgatagtg acagcgatgg
    17401 ctgtaatcag cctctttact ctgatgggat gtaataaccg ggccgaagtc gatacgcttt
    17461 ctccagcaca ggctgccgaa ctgaaaccga tgccgcaaag ttggcgcggc gtgctgccgt
    17521 gtgctgattg cgaaggaatc gaaacctctc tgttcctcga aaaagacgga acatgggtga
    17581 tgaatgagcg ttatctcggt gctcgtgaag aaccttcctc cttcgcttcc tacggtacat
    17641 gggcgcgaac cgctgacaag ctggtattaa ccgacagcaa aggtgaaaag tcatattatc
    17701 gggcgaaagg cgatgcgctg gagatgctcg atcgtgaagg caatccgatt gaatcgcagt
    17761 tcaactatac gctggaaccg gcacaatcta gtttacctat gacgccgatg accctgcgag
    17821 gcatgtattt ttatatggct gatgcggcga ccttcactga ttgcgcgacc ggaaaacgtt
    17881 tcatggtagc gaataacgca gagctggagc gtagctacct ggctgcgcgc ggtcacagtg
    17941 aaaaaccggt gttactgtca gtagaaggtc actttacgct tgaggctaat ccggatactg
    18001 gtgcgccaac caaagtatta gcgcccgata cggcgggtaa attttacccg aataaggatt
    18061 gcagtagttt ggggcagtaa acctgcttaa ggcagaaaca aacgcaaaat tgcctgatgc
    18121 gctgcgctta tcaggccaac agcatataat gcaacatttt gaattagcac gattttgtag
    18181 gccggataag gcgtttacgc cgcatccggc attttgtgca cgaggctggc tttatcactg
    18241 agtcagccca acaaagcgac ccgccttgat ataaatccca ggcttcaggt gcccaacata
    18301 ctgtaactgc gtttctggct taaacgcaga gacatcacca atacgcatat gcatcagatc
    18361 ggcagggcta atccagcccg attgcgccag tgtaagtgga tgcccggctt tggcaaaggc
    18421 atccgtgaca aattccgaac aaaaccacga ctttttgtct ccttcgccca cactgcttaa
    18481 ctgcgctttc gccaggccgc tgacgcactt tggcgaaaat cctcggagaa cggattcagt
    18541 gagcacatct ggcgagtcac cataaaggga ataaattcga caatgccgcg atagttataa
    18601 ccgctatctt tgattttatt ggcaaacgcg gtgatatctg tggcttgttg cggggtaaga
    18661 tccgggactc gtaagacgaa aagcttatca ctatgcttca tcgctttttt aagggaaaca
    18721 atctggacgc cagcgcctgt cgcttctgca acgttattat cacccagaaa gattgcaacg
    18781 tgactcacag aggaagtgct gaagacgcgg attccaaatg aggttacccc aaggcttgag
    18841 gagaacagca aatcgccggg tttgagatca ggtgccgtta tttcttttat tgattgttcg
    18901 gtgaaagagc tttgatgctg gaatttgaca gcccatgttt ttgcctcggc atctactgct
    18961 gtggctgatg ggtctggctg gctgatatca acggtacaag ccgaaagtaa aagaaagctg
    19021 gggagaaaca ggcggcagta cgcctttggt ttatccattt tatacaatcc atgtaaaaaa
    19081 agggccctga aattcaggac cctttctggc atcagccttt aatctgtttc accagatatt
    19141 cgacgatgtc accagtctta attaactgtt tctcgccgtt gcgacgatat ttatattcga
    19201 tatcgtcgtt gtcgaggtta cggtcgccca gcacaatagt gtgcggaata ccgatcagtt
    19261 ccatatcagc aaacatcacg cccggacgct ctttgcggtc atccagcagc acttcgatac
    19321 cttgtgcacg cagttcgctg tacagtttct cagcaagctc ttgtacgcgg aaggatttgt
    19381 gcatgttcat cggcagaatc gccacctgga acggcgcgat agcgtcaggc catacgatgc
    19441 ctcgttcgtc gtagttctgc tcaatcgccg cagctaccac acgcgttacc ccgataccgt
    19501 agcaacccat cgtcaggatt tggttacggc catcttcacc ctgtacggag gctttcagtg
    19561 cttcggagta cttggtaccc agctggaaga tgtgaccaac ttcgatacca cgtttgatca
    19621 gcagcgtacc ctggccatcc gggcttggat cgccagccac cacgttacgg atatctgcaa
    19681 cttccggggt agcgacatcg cgatcccagt tgatgccgaa gtagtgttta ccatcgatgt
    19741 tagcaccagc agcgaaatca ctcatcgccg caacggtacg gtcaatcacc accggaatcg
    19801 gcatgtttac cggacccagt gaacccggac cggctttaac cacggcacga atttcttctt
    19861 cggtcgcgaa agtcagcggg cttgcaacct gcggcagttt ttctgcttta acttcgttca
    19921 gctcgtgatc accgcgcacc agcagcgcaa ccagcgggaa gctactgcct tcaaccgctt
    19981 taaccagcag agtcttaacc gttttctcaa tcggcagatt gaactgttca accagttccg
    20041 cgatggtttt cgcgttcggc gtatcaacca gcgtcatttc ctgggtagca gcagcgcgcg
    20101 gttctttcgg cgcgatagct tctgccagtt caatgttcgc tgcatagtca gaggtgtcgg
    20161 agaagaccac atcgtcttca ccgctctgcg ccagcacctg gaattcgtga gaggcgctgc
    20221 cgccgataga accggtgtcg gcttgtacgg cgcggaaatc cagccccatg cggctgaaga
    20281 ttttgctgta ggccgcatac attgcatcgt aggtttcctg cagggattcc tgagaagtat
    20341 ggaaagagta agcatctttc atcaggaatt cgcgggaacg catgacgccg aaacgcggac
    20401 gcacttcgtc gcggaacttg gtctggatct gatagaagtt cagcggcagc tgtttgtaag
    20461 agctaagctc gttacgaatc aggtcagtga taacttcttc atgagttggg ccgagtacga
    20521 acggacgctc gccacggtca acaaaacgca gcagttccgg accgtactgt tcccaacgac
    20581 cactctcttg ccacaaatca gctggctgaa ccaccggcat cgacacctcg atcgcaccgg
    20641 cgttgttcat ctcttcacgc acgatgtttt cgactttttt cagaacgcgc acgccggtcg
    20701 gcagccaggt atataacccg gaggccagct tgcggatcat cccggcgcgc agcatcagct
    20761 gatggctgat cacctcggcg tcggcaggtg tctccttgag agtggagagc aggtattggc
    20821 tagtacgcat gttgttacgg ttccagttgg aaggtagaac aggctcaagg cgagcctggg
    20881 acaaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa
    20941 tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt
    21001 taaaatcatg tagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg
    21061 ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct
    21121 ccagcgtcaa aagctgcttt tcgacctctg cggtaaaact caccgccatc tctgccgctg
    21181 gcgcactttg cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg
    21241 gtttgatatc cactaccggc gtaccatcga ccagatccag actgccgagc ttcagaatca
    21301 cgctgtcttt atggcaaaca acctctttca gctctaccag cgacatacca attgggttag
    21361 ggcggaaagt agagcgtgtt gcgaaaaccc ccattctggc gttaccgccg aggcgcggtg
    21421 gacgcacagt cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata
    21481 aatggctgaa cgcttccagg ccgcgaacgg cgtcggcctg gttgtaggga gcaatgagat
    21541 gcagttcgcc gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt
    21601 tatagggcga gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat
    21661 gttaagcgca gagccgatac atacagcctg acgatagcag cctggcgtac cgctggtgac
    21721 ttcgcagcta tgcagtaata ccgcattggc cttcattttc gaggcgttga tttgcatccg
    21781 cttacgcgcg gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc
    21841 actgacttca ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat
    21901 tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc
    21961 agtgctttga acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag
    22021 tgctactaaa cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc
    22081 aatattgaat caggagcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata
    22141 ttacaaattg tggaaacagc ctaaaaatta ccagccttta acagcgccgc cgttaaacac
    22201 tttgtttgct gcttcgtaaa cttcatcaga ctgataagcc tggacgaatt tcttcacgtt
    22261 ctcggcgtct ttgttatctt cacgcgttac gatcaggttt acgtacgggg agtctttatc
    22321 ttcgacaaag ataccgtctt tcgctggagt caggccaatc tggctggcat aggtggtatt
    22381 gataactgcc agagcgattt gcgcgtcgtc cagagatcga ggcagctgcg gtgcttccag
    22441 ctcaacaatt ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc
    22501 aacgccatct ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag
    22561 attagttgga tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt
    22621 gattttcttg gagtaaccag caatcggata aacaaaagtg ttgcctactg cgaccagttt
    22681 gtagccacga tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc
    22741 gatatcgcct ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc
    22801 aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg acttctgcta cctgctgttc
    22861 ggcaccaaca atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc
    22921 taccagtgcc agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc
    22981 cataccttat tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc
    23041 ccggacgatg cggtcgcctg cgaactgaat taaataaacc agaatgacca gcaataccag
    23101 taccgtattc atcaccgtcg cgttatagcc gatgtagcca tactgatagc caatctgacc
    23161 taaaccaccg gcaccgactg caccacccat cgcggaataa ccgaccaggg taatcagggt
    23221 gatagttgcc gcattcacca gacccggcaa tgcttccggt aacagcactt tacggacgat
    23281 ctgcatcggc gtggcaccca ttgcacggga agcttcaatt aacccggttg ggatctccag
    23341 cagagcgttc tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat
    23401 cgctgcctgc aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac
    23461 cagcaagata atgaacggga tggaacggaa aatgttcaca atcgcagaaa cggtacgata
    23521 cagcttcgcg ttagcaataa tttgccccgg gcgcgtaaca taaagcagga cgccgaccgg
    23581 cagaccaatc acaaagccaa aaaatccgga tacgaaggtc attgccagcg tttcccatac
    23641 gccacgaacc agcaaccaca tcatcggctc agacataacc cagtacctct acttttacat
    23701 ggtgttcctg cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag
    23761 tcagcatgat gccgaactta acgccaccgg cgtaatccat ctgcgcgcta ataatgttgt
    23821 tgttgacgtt gaaacgacgc gcggtttcag aaagcagcgg ggcatcgacc gattgaccgg
    23881 taaactccag gcgcaacatc ggcacgcagt cagtaaatgg ctccgcttgc agacgttcct
    23941 ggtaatcttc cgggatatcc
//
LOCUS       DAEVZA010000074        23496 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEVZA010000074 DAEVZA010000000
VERSION     DAEVZA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.074"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            118..1632
                     /gene="caiT"
                     /locus_tag="LAF92_002931"
     CDS             118..1632
                     /gene="caiT"
                     /locus_tag="LAF92_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBI9791902.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1663..2805
                     /gene="caiA"
                     /locus_tag="LAF92_002932"
     CDS             1663..2805
                     /gene="caiA"
                     /locus_tag="LAF92_002932"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBI9791903.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLLPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2934..4151
                     /gene="caiB"
                     /locus_tag="LAF92_002933"
     CDS             2934..4151
                     /gene="caiB"
                     /locus_tag="LAF92_002933"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBI9791904.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4225..5778
                     /gene="caiC"
                     /locus_tag="LAF92_002934"
     CDS             4225..5778
                     /gene="caiC"
                     /locus_tag="LAF92_002934"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBI9791905.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5887..6672
                     /gene="caiD"
                     /locus_tag="LAF92_002935"
     CDS             5887..6672
                     /gene="caiD"
                     /locus_tag="LAF92_002935"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBI9791906.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6678..7268
                     /gene="caiE"
                     /locus_tag="LAF92_002936"
     CDS             6678..7268
                     /gene="caiE"
                     /locus_tag="LAF92_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBI9791907.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7354..7749)
                     /gene="caiF"
                     /locus_tag="LAF92_002937"
     CDS             complement(7354..7749)
                     /gene="caiF"
                     /locus_tag="LAF92_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBI9791908.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8010..11231)
                     /gene="carB"
                     /locus_tag="LAF92_002938"
     CDS             complement(8010..11231)
                     /gene="carB"
                     /locus_tag="LAF92_002938"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBI9791909.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQRAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11249..12397)
                     /gene="carA"
                     /locus_tag="LAF92_002939"
     CDS             complement(11249..12397)
                     /gene="carA"
                     /locus_tag="LAF92_002939"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBI9791910.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12853..13674)
                     /gene="dapB"
                     /locus_tag="LAF92_002940"
     CDS             complement(12853..13674)
                     /gene="dapB"
                     /locus_tag="LAF92_002940"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBI9791911.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(13841..14755)
                     /gene="rihC"
                     /locus_tag="LAF92_002941"
     CDS             complement(13841..14755)
                     /gene="rihC"
                     /locus_tag="LAF92_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBI9791912.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(14821..15771)
                     /gene="ispH"
                     /locus_tag="LAF92_002942"
                     /gene_synonym="lytB"
     CDS             complement(14821..15771)
                     /gene="ispH"
                     /locus_tag="LAF92_002942"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBI9791913.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15773..16222)
                     /gene="fkpB"
                     /locus_tag="LAF92_002943"
     CDS             complement(15773..16222)
                     /gene="fkpB"
                     /locus_tag="LAF92_002943"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI9791914.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16310..16804)
                     /gene="lspA"
                     /locus_tag="LAF92_002944"
     CDS             complement(16310..16804)
                     /gene="lspA"
                     /locus_tag="LAF92_002944"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBI9791915.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16804..19620)
                     /gene="ileS"
                     /locus_tag="LAF92_002945"
     CDS             complement(16804..19620)
                     /gene="ileS"
                     /locus_tag="LAF92_002945"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBI9791916.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19663..20604)
                     /gene="ribF"
                     /locus_tag="LAF92_002946"
     CDS             complement(19663..20604)
                     /gene="ribF"
                     /locus_tag="LAF92_002946"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBI9791917.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20612..20830)
                     /locus_tag="LAF92_002947"
     CDS             complement(20612..20830)
                     /locus_tag="LAF92_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBI9791918.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20933..21196
                     /gene="rpsT"
                     /locus_tag="LAF92_002948"
     CDS             20933..21196
                     /gene="rpsT"
                     /locus_tag="LAF92_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBI9791919.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            21680..23101
                     /gene="espX1"
                     /locus_tag="LAF92_002949"
     CDS             21680..23101
                     /gene="espX1"
                     /locus_tag="LAF92_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HBI9791920.1"
                     /translation="MTNGISTSPHCLYKSNIVDDVIINKTRQNELVKVFCEYKIEFLI
                     LFDDFFRSHNLPKPSPVLHHFFQYTHLRDAYFYRCKLIEHTVQFYFFKHKGITLLRLD
                     VFDDRTSECLSEEIKIYQECHEKFVNFLKANFNQEIYPQLYTPEIFYEACRNLQSFYD
                     HQETSQNAKYSAIDKKKSHLNQEIKKLIKKNIFPELYNEQCNKIPSSSIDDNQGIMWQ
                     NFKTSNIAYSQLCKKLSLFKSIPSRLIKKSAYCSTENTITNKFDVVFSYCGNNVKEFI
                     LLLPYNKSLEMHELNEQNIQYLTALNINIHKMLLSNITIEKSDLSYGYYFGCVLSNIL
                     CFESDLSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCEDIMPGRWTTQLVNKHL
                     GYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSS
                     EMRKYLKSINECMFLMKNIAHQN"
     gene            23348..23496
                     /locus_tag="LAF92_002950"
                     /pseudo
     CDS             23348..23496
                     /locus_tag="LAF92_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         5879 a         6126 c         5810 g         5681 t
ORIGIN      
        1 attatgagtt agccattaac gcgtccacga ggttaataat aattatatta aatgttaaca
       61 aaaataaaac aaacgggaac gcaaaataaa tattcgtttt cacagtggaa ttaactcatg
      121 aagaatgaaa agagaaaaac gggaatagaa ccgaaggttt tctttccgcc gttaataatc
      181 gtcggcatac tttgttggct tacagtcaga gatctggatg cagcgaatgt cgttattaat
      241 gctgtattca gttacgtcac caatgtatgg ggatgggcat ttgaatggta tatggtggtg
      301 atgcttttcg gttggttctg gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac
      361 gaaccacctg aatttagtac cgccagttgg atctttatga tgttcgcctc ctgtacgtct
      421 gctgccgtac tgttctgggg atcgattgag atctactact acatctccac cccgccgttt
      481 ggcttagaac cgaactcgac aggggcgaaa gagttggggc tggcttacag cttgttccac
      541 tggggacctc tgccgtgggc cacttacagc ttcctttcag tcgccttcgc ttacttcttc
      601 tttgtccgca aaatggaagt gattcgcccc agctcgacac tggtgccgct ggtaggtgaa
      661 aaacacgcca aagggttgtt cggcactatc gtcgacaact tctatctcgt cgccttgatc
      721 ttcgcgatgg gtaccagtct gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg
      781 ttgtttggca ttccgcatac cctgcaactg gacgctatca tcattacctg ctggattatc
      841 ctcaacgcca tttgcgtcgc ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg
      901 cgtagttacc tgagcttcct gatgctgggt tgggtgttca ttgtcagcgg tgccagcttc
      961 atcatgaact acttcaccga ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg
     1021 ttctataccg atcccatcgc taaaggcggc ttcccgcagg gctggaccgt gttctactgg
     1081 gcatggtggg tgatttatgc tatccagatg agtatcttcc tcgcccgcat ctcccgtggt
     1141 cgtactgtgc gtgaactgtg cttcggcatg gtgctggggc tgacagcgtc aacctggatc
     1201 ctgtggactg tactcggtag taacactctg ctgttgatag ataaaaacat catcaacatt
     1261 ccaaatctga tcgaacagta cggtgtggcg cgcgccatca ttgaaacctg ggccgctctg
     1321 ccactcagca ccgccaccat gtggggcttc ttcatcctct gctttattgc caccgttacg
     1381 ctggttaacg cctgctctta taccctggcg atgtccactt gccgcgaagt acgcgatggt
     1441 gaagaaccac ctctgctggt gcgtatcggt tggtcaattc tggttggcat tatcggtatt
     1501 gttctgctgg cgctcggcgg cctgaaaccg attcaaaccg ccattatcgc cggaggatgc
     1561 ccgctgttct tcgtcaacat tatggtgacg ctctccttta ttaaagacgc gaaacagaac
     1621 tggaaagatt aattaacccc caaaatatca agaggttgaa agatggattt taatttaaat
     1681 gatgagcagg aactgtttgt cgccggtatc cgcgaactga tggccagcga aaactgggag
     1741 gcctattttg ccgagtgcga ccgtgacagc gtctacccgg aacgttttgt caaagcactg
     1801 gcggatatgg gtatcgacag tctgctgctc cctgaagagc acggtggtct ggacgcgggg
     1861 tttgttactc tcgccgccgt gtggatggag ctgggacgtc tgggggcacc aacctatgtg
     1921 ctgtaccagt tgccgggcgg gttcaacacc ttcctgcgcg aaggcacaca agagcagatc
     1981 gacaaaatta tggctttccg cggcaccggt aagcagatgt ggaactcagc gattaccgaa
     2041 ccgggcgcgg gctccgacgt gggtagcctg aaaacgactt atacccgtag aaatggtaag
     2101 atttatctta atggtagtaa gtgttttatt accagcagcg cctatacccc gtacatcgtg
     2161 gtgatggcgc gcgacggggc ttctccggac aaacctgtct acaccgaatg gtttgttgat
     2221 atgagcaaac cgggcatcaa agtgaccaaa cttgaaaagc tcggtctgcg tatggatagc
     2281 tgctgtgaaa tcacctttga cgacgtggaa ctggacgaga aagacatgtt cggtcgggaa
     2341 ggtaacggct ttaaccgcgt caaagaagag ttcgaccatg aacgtttcct ggtagccctc
     2401 accaactacg gtacggcgat gtgcgccttt gaagatgcgg cgcgctacgc caatcagcgc
     2461 gtgcagtttg gcgaggctat tggtcgtttc cagttgattc aggaaaaatt cgcccacatg
     2521 gcgatcaaat taaactccat gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac
     2581 ggcaccatca cctctggcga tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt
     2641 gaagtggtgg atagcgcaat gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc
     2701 atcagccgct tctggcgtga cctgcgtgta gaccgcgtct ccgggggatc tgacgaaatg
     2761 cagatcctga cgctgggtcg tgcggtgctg aagcaatacc gctaagtcaa tgcgctgcct
     2821 gatgcgacgc tgatgcgtct tatcaggcct acgggagcac gaatgtaggc cggataaagc
     2881 gtttacgccg catccggcag tcatgcgcta cataaatttt tcaggagcaa ataatggatc
     2941 atctacccat gccgaaattc gggccgttgg ccggattgcg cgttgtcttc tccggtatcg
     3001 aaatcgccgg accgtttgcc gggcaaatgt tcgcagaatg gggcgcggaa gttatctgga
     3061 tcgagaacgt cgcctgggcc gacaccattc gcgttcaacc gaactacccg caactctccc
     3121 gccgcaattt gcacgcgctg tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc
     3181 tgaaattaat ggaaaccacc gatatcttca tcgaagccag taaaggtccg gcctttgccc
     3241 gtcgtggcat taccgatgaa gtactgtggc agcacaaccc gaaactggtt atcgctcacc
     3301 tgtccggttt tggtcagtac ggcaccgagg agtacaccaa tcttccggcc tataacacta
     3361 tcgcccaggc ctttagtggt tacctgattc agaacggtga tgttgaccag ccaatgcctg
     3421 ccttcccgta taccgccgat tacttttctg gcctgaccgc caccacggcg gcgctggcag
     3481 cactgcataa agtgcgtgaa accggtaaag gcgaaagtat cgacatcgcc atgtatgaag
     3541 tgatgctgcg tatgggccag tacttcatga tggattactt caacggcggc gaaatgtgcc
     3601 cgcgcatgag caaaggtaaa gatccctact acgccggttg cggtctgtat aaatgcgccg
     3661 acggctacat cgtgatggaa ctggtgggca ttacccaaat tgaagagtgc tttaaagata
     3721 ttggcctcgc acatctactc ggtacgccgg aaatcccgga aggcactcag cttatccacc
     3781 gtatcgaatg cccttacggc ccactggtag aagaaaaact cgatgcctgg ctggcggcac
     3841 ataccatcgc agaagttaaa gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac
     3901 tgaccgtacc ggaactggaa agcaatccac agtatgttgc ccgtgaatca atcactcagt
     3961 ggcaaacgat ggatggtcgc acctgcaaag ggccgaacat catgccgaaa ttcaaaaata
     4021 accccggaca aatctggcgc ggaatgccct cacatggcat ggacacggct gccattttga
     4081 aaaatatcgg ctacagcgaa aacgacattc aggagttggt cagcaaaggt ctggccaaag
     4141 ttgaggacta aacgcgtggc gtcagtcggc ttgcctgact agcgcccgcc caataacaaa
     4201 cgaacaaaaa tggatagagg tgcaatggat atcattggcg gacaacatct acgtcaaatg
     4261 tgggacgatc ttgcggacgt ttacggtcat aaaacggcgc tgatttgtga atccagcggc
     4321 ggagtcgtta accggtatag ttatcttgag ttaaatcagg agattaaccg cacggcaaac
     4381 ctgttttata cgctggggat tcgcaaaggc gacaaggttg cactacatct cgacaactgc
     4441 ccggaattta tcttttgctg gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt
     4501 aacgcccgcc tgttacgcga ggaaagcgcg tggatcctgc aaaatagcca ggcgtgcctg
     4561 ctggtgacca gtgcgcaatt ctatcctatg tatcaacaga ttcagcagga agatgccact
     4621 caattgcggc acatttgcct gacagatgtg gcacttcccg ctgatgatgg cgtgagttcg
     4681 tttactcaac tgaaaaatca acaacctgcc accttgtgct atgcaccgcc gctattgact
     4741 gacgatacgg cggaaattct cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg
     4801 attacccatt acaacctgcg cttcgctgga tattactccg cctggcagtg tgcactgcgt
     4861 gacgatgacg tctacctgac ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg
     4921 gcgatggcgg cgttttctgc cggggccacc tttgtgctgg tcgagaaata cagcgcccgc
     4981 gccttctggg gacaggtgca gaagtaccgc gccaccatta ccgaatgtat tccgatgatg
     5041 atccgtacgt tgatggtgca gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa
     5101 gtgatgtttt atctcaactt gtcggagcag gaaaaagatg cgttttgtga acgcttcggc
     5161 gttcgcttgc tgacgtctta tgggatgacg gaaaccattg tgggcattat cggcgatcgc
     5221 cctggcgata aacgacgctg gccgtcgatt ggtcgggcgg ggttttgcta cgaagcggag
     5281 atccgcgacg atcacaatcg cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa
     5341 ggcgtaccag ggaaaaccat cttcaaagag tattttctca acccgaaagc cactgcgaaa
     5401 gtgctggaag ccgacggctg gctacatacc ggcgataccg gatactgcga cgaagagggc
     5461 tttttttatt tcgtcgatcg ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc
     5521 tgcgtggagc tggaaaatat catcgccacc catccaaaaa ttcaggatat cgtggttgtg
     5581 ggtattaaag attcgattcg cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt
     5641 gaaacattga gcgaagagga atttttccgc ttctgcgaac aaaatatggc gaaatttaaa
     5701 gtgccctctt atctggagat cagaaaagat ctgccacgta attgctcggg gaaaataatt
     5761 agaaagaatc tgaaataaat gaaacagcag ggaactaccc tgccagctaa taatcacgct
     5821 ataaaacaat atgcattttt tgcagggatg ttgtcgtccc tgaaaaagca aaaatggaga
     5881 aaaggaatga gtgaatcatt acatctgacc cgcaatggat caattctgga aattaccctt
     5941 gatcgtccaa aagcgaatgc tattgatgca aaaaccagct ttgaaatggg cgaagtattt
     6001 ctaaatttcc gtgacgatcc gcaattacgt gtcgccatta ttaccggtgc cggagagaag
     6061 ttcttttccg cgggctggga tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac
     6121 tttggcccgg gtggatttgc gggattaacc gaaattttca atctcgacaa accggttatc
     6181 gcagctgtga acggctatgc ctttggcggc ggtttcgaac tggcgctggc ggcagatttt
     6241 attgtttgtg ccgataacgc cagcttcgcc ctgccggaag ccaagctggg catcgttcct
     6301 gacagcggcg gcgtgctgcg tctaccgaag atcctgccgc ctgccatcgt caatgaaatg
     6361 gtgatgaccg gcagacgaat gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc
     6421 gtggttagcc aggcggaact gatggataac gcccgcgaac tggcgcagca gctggttaac
     6481 agcgccccgc tggcgattgc ggcgctgaaa gagatcttcc gcaccaccag cgaaatgccg
     6541 gtagaagaag cgtatcgcta tattcgcagc ggcgtgttga aacactatcc gtcggttctg
     6601 cattcggagg atgccattga agggccgctg gcgtttgccg agaagcgcga tccggtgtgg
     6661 aaaggacgtt aacaaccgtg agctattacg cctttgaggg gttaattccg gtggttcacc
     6721 cgacggcgtt tgtccatccc agtgccgtct tgattggcga tgtgattgtg ggagccggtg
     6781 tctacatcgg cccactcgcc tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg
     6841 gagccaatat tcaggatggc tgcattatgc atggctactg cgacactgac accatcgttg
     6901 gggaaaacgg ccatatcggg cacggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg
     6961 cattggtcgg gatgaacagc gtgattatgg atggcgcggt cattggcgaa gagagcattg
     7021 ttgccgccat gagctttgtc aaagcaggct ttcacggcga gaaacgccag ttgttgatgg
     7081 gtacgccagc ccgcgctgta cgcagtgtta gtgacgacga gttacactgg aaacggttga
     7141 ataccaaaga gtatcaggat cttgtcgggc gctgtcatgc atcgttacat gaaacacagc
     7201 cactgaggca aatggaggaa aatcgccccc gtttgcaggg gacgacggat gtgacgccta
     7261 aacggtgatc ttgccggatg cgacctggtt gctggataag atgctacaac atcgcatctg
     7321 gcaagcagga cgcccggtat caacgagata aaattaacga cgcatactct ttgacaacat
     7381 catctgccac ttctgctcgc ggttaagttc cgtgagcgga acggcaggaa tacgcggcgt
     7441 ctttcttacg ccgactaatt tatcccggct gttattgcgc aggcgcgcgt aaatttgctc
     7501 atcgatatcg accactttta ccagtcgctg gcactggcat ccccgtcctt ccagcttatt
     7561 agggatcatc ttaacttcgc agcttatttc tgcgacttcc gacagaatat aagtcagggt
     7621 attaaccgcc ttgctgtgtt caatatcgaa atgaatagag atctctcttg ctatcaccca
     7681 ccgattttca gccatcatcc attcggcgat taacaagtag agtggttttt caacatatcc
     7741 ttcacacata cgaactccag tttcacaaat taaaagtctg gctatgactc gttttgaagc
     7801 aattaaacaa ggccatatta aaattgtgga tatatcgata gtatgcaact tcaccgtcga
     7861 taagtgtgag ctaaatcctg cttatgtcat caaaaatatc gattccccaa aagatttact
     7921 taatgggcaa aaaaaacggt cctcatcaga gaaccgtatt acttattcga tcaaattagc
     7981 ggatgaaaaa tatccgccat gacacgctat tatttgatct gcgcgtgcat ttcctgcacc
     8041 gaaattactt tttcggtcgc gtcggcattc agcgccatcg cggtagcgaa accaccgttc
     8101 agggtggtgt cataatgcac tttatattgc agcgcactgc gacggatcac gcgggagtct
     8161 tcaatcgcgc gacggcctga agtggtgttg atgatgtagg tatattcgcc attcttgatg
     8221 cggtcctgaa tgtgcggacg gccttcatgc accttgttca ccagacgcgg attaatgccc
     8281 gcttcgccca gcacaatcgc cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc
     8341 agttttgccg ccaggtccac cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca
     8401 cgaccgtgtt tcttcatggt ggagttgctg cccagctgcg ctttggcaaa cgcttcagcg
     8461 aaggtgcggc ccacgcccat cacttccccg gtggagcgca tttctggccc taacagcggg
     8521 tcaacacccg ggaatttatt gaacggcagc accacttctt tcaccgagta gtacggcggg
     8581 ataacttctt tggttacgcc ctgctcagcc agcgatttgc cagccatcac acgcgccgcc
     8641 acttttgcca gcggtacgcc ggtggctttg gagacgaacg gaacggtacg cgccgcacgc
     8701 gggttaactt caatcaggta gacttcgttg tttttcaccg caaactgcac gttcatcaga
     8761 ccgcgcacct gcaattcgaa ggccagtttc tgcacctgct ggcgcatcac atcctgaatt
     8821 tcctgactta aggtgtaggc tggcagagaa catgcggagt caccggagtg cacgcccgcc
     8881 tgctcgatgt gctccatgat gccgccaatc agcaccattt cgccgtcgca gatggcatcc
     8941 acgtcaactt ctaccgcgtc atcaaggaag tgatccagca acactggcgc atcgttagac
     9001 acgctgaccg ccgtctggaa gtagcgacgc aggtcagctt cgtcatagac gatttccatc
     9061 gcccgaccgc cgagaacgta agacggacgt accaccagcg ggtagccaat ctctttcgcc
     9121 ttctctaccg ccatttcaat agcggtaacg gtggcgttcg ccggttgttt cagtttcaga
     9181 cgctcaaccg cacgctggaa gcgttcacgg tcttctgcac ggtcgatagc atccgggctg
     9241 gtgccgataa ccggtacgcc agcagcttcc agcgcgcgcg ccagtttcag cggggtctga
     9301 ccgccgtact gaacgataac gcctttcggc ttctcaatac gcacgatttc tagcacatct
     9361 tccagagtta ccggctcgaa gtagaggcga tcggaagtgt cgtagtcggt ggagacggtt
     9421 tccgggttac agttaaccat aatggtttcg taaccgtctt cgcgcagcgc cagcgaggcg
     9481 tgtacacagc agtagtcgaa ttcgataccc tgaccgatac ggttcgggcc gccgccgaga
     9541 accatgattt tttcacggtc ggtagacgga ttcgcttcgc actcttcttc ataagtggag
     9601 tacatgtaag cggtgtcggt ggcgaactct gccgcacagg tatccacgcg cttataaacc
     9661 gggtgcaggt catactggtc gcgcagcttg cggatttccg cttcgcgcac gcccgccagt
     9721 tttgccagac gcgcatcggc aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc
     9781 aggccagtga tgcccacttc cgctactttc tcttccagac gcaccagttc ttcaatctgt
     9841 accaggaacc agcggtcaat attggtcagg ttgaacacgc cgtccacaga caggcccgca
     9901 cggaaggcat cggcgatgta ccagatacgc tctgcgccag catctttcag ttcgcgacgg
     9961 attttggtta acgcttccgg gtcatcgagg ctcactttcg ggtcgaatcc agtcgcaccg
    10021 acttccaggc cgcgcagcgc tttttgcagg gattcctgct gcgtgcgacc aatcgccatc
    10081 acttcgccaa ccgatttcat ctgagtggtc agacggtcgt tagcaccagc gaatttttcg
    10141 aagttgaagc gaggaatttt agtaaccaca tagtcgatgg acggctcgaa ggaggccgga
    10201 gtacgtccgc cagtgatgtc gttcatcagt tcgtcgaggg tgtaacccac cgccagtttc
    10261 gccgccactt tagcaatcgg gaaaccggtc gctttcgacg ccagcgccga agaacgggac
    10321 acgcgtgggt tcatttcgat aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg
    10381 acattggaac caccggtttc aacgccgatt tcacgcagca ccgccatcga ggcgttacgc
    10441 atgatttgat attctttgtc ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg
    10501 gtatggatgc ccatcgcatc aaagttttcg atagagcaga cgatgatgca gttgtcgttt
    10561 ttatcacgca ccacttccat ctcgtactct ttccagccga tcagcgactc atcaatcaac
    10621 agctctttgg ttggggagag atccagaccg cgggcgcaaa tttcttcaaa ctcttcgcgg
    10681 ttataagcga taccgccgcc gctaccgccc atggtaaagg atgggcgaat aatgcacggg
    10741 aagcccacgt cagcggcaac cgccagcgct tcttccatcg tgtgtgcgat accggaacgc
    10801 gcggtttcca gaccaatttt cttcatcgct acgtcgaaac gacggcggtc ttctgcttta
    10861 tcaatcgcat cggcggtcgc accaatcata gtcacgccga actcttccaa cacgccctga
    10921 cgctccagct ccagcgcgca gttcagcgcc gtctgaccgc ccatcgttgg cagcaccgcg
    10981 tccgggcgct ctttttcaat aatcttgcgt actacttccc agtgaatcgg ctcgatgtag
    11041 gtcgcatcgg ccatttccgg gtcggtcatg atggtcgccg ggttggagtt caccagaatg
    11101 acgcggtaac cctcttcgcg cagggcttta catgcttgcg cgccagagta gtcaaactca
    11161 cacgcctgac cgataacaat cgggcccgca cccagaatca ggatactttt tatatctgta
    11221 cgttttggca tggctctttt actcctgatt acttagcggt tttacggtac tgctcaatta
    11281 actcgataaa gtggtcgaac aacggcgctg cgtcgtgtgg cccagggctg gcttccgggt
    11341 gaccctggaa gctgaacgcc ggtttatcag tgcgatgaat gccctgtaac gtaccgtcga
    11401 acagggattt atgcgtgaca cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac
    11461 cgtggttctg ggcggtgatc atcaccacgt ttttctccac atctttaacc ggatggttgc
    11521 cgccgtggtg accaaatttc attttgacag tcttcgcacc gctcgccagc gccagcagct
    11581 gatgaccgag gcagatgccg aagaccggaa tatcggtttc gaggaatttc tggatggcgg
    11641 taatggcgta atcgcacggg gccgggtcgc caggaccgtt ggagaggaag atgccgtctg
    11701 gattcatttt cagcacatct tccgcagaag tttgcgccgg aacgatggtc aggcgacagc
    11761 ctctgtccac caacatgcgc aggatgttgc gcttggcacc aaaatcataa gccacgacgt
    11821 ggaacggcag ctcgtcttct tttttcgctt ctggcaggcc gccggtcaac gtccagctcc
    11881 cttgtgtcca gctataggct tctgcggtgg tcacttcttt tgccagatcc atgccattca
    11941 gacctgggaa cgcgcgggct ttttctaacg ccaacgccgc atccgggtta tcgcctgcga
    12001 taatgcagcc attctgtgcg cctttctcgc gcagtaaacg cgtcagctta cgggtatcga
    12061 tatcggcaat cgccacgatg ttatggcgct tcaggtaaga agagaggtct tcggtattac
    12121 ggaagttgct ggcaatcagc ggcaggtcgc gaatcaccag accttgtgca tgtacctgag
    12181 aagattcttc atcggcgtca ttagtgccga cattgccaat atggggataa gtaagagtaa
    12241 cgatttgacg agaataggaa ggatcagtga ggatttcttg ataaccggtc attgaagtat
    12301 tgaaaacgac ttccccaacc gccgaacctg ttgcccctat ggcccgaccg tgaaactggg
    12361 ttccgtcttc cagaaccaat agcgctgact taatcaaaac accctccaga gaatattcac
    12421 tcactttatt tgcatattaa ttcacaatga tgacgtaaat caatgcaaat ctgcttaccg
    12481 acgaatttct ggcaaacggc ggcattctgg agatatgggc gctaaaagtc aacttaatgg
    12541 cgggattttt tatattattt tgtggctcat gcatgtttta caggcattaa gcggcaaaaa
    12601 gatcatataa cctgttctgg aaaacgcttg cgcagcaaga agattgcatg aaataaaagc
    12661 gagtagggaa aagtggacca aatggtcaaa atttacatta aaacaacaaa aaccgaagac
    12721 aaaacatgta atttttggca cttatagaca atttaaaaac ataacactaa aaataaaagg
    12781 gccaaaaatt aaagcccttt acaatcaata aattaaatgt gttatttttg caccataaca
    12841 aatattttgt ggttacaaac tattgagatc aagcacatct cgcatatcaa aaagaccact
    12901 ttccttacca ctcaaccaca aagccgatct taccgcgccg ttagcaaatg tcatgcggct
    12961 ggacgcctta tgggtgatct ccagacgctc gccaatatcg gcaaacatcg cggtatgttc
    13021 gccaacgatg tcacctgcac gcacggtggc aaaaccaatg gtgccaggca cacgttcacc
    13081 ggtgtggcct tcacgactgt agaccgcgca atctttcaga tctttatcaa gggcgtgggc
    13141 gatcgcctct cccattgcca gtgcggtgcc tgacggcgca tcaactttat gtctatgatg
    13201 tgcttcaata atttcgatat cggtgtagtc acccatcact ttggctgctt tctccagcag
    13261 cttaagcatg acgttaacgc caacgctaaa attggcggca aagacaatcg caatatcggc
    13321 ggcggcgtca cgaatcgcct gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat
    13381 ccctttgcca tgctggcgac aaaaagcgag atggttcagc gtaccttccg gacgggtaaa
    13441 atcgataaac acatcaaaat catcttttac cgcatcgagg ctgctttgca cggtaacgcc
    13501 tgttttccct gcaccagcca gctcaccggc gtcgctgccc agtaaagaag atccttcgcg
    13561 ctccagcgcc gcgcccagct gcacgccttc taatgccagc gccgcctgaa tcaactggcg
    13621 gcccatacgc cccccggctc ccgcgatggc aacgcggatg tttgcatcat gcatagctat
    13681 tctcttttgt taatttgcat agaccgtttt cagagtaacc agcgcatcgc tacgccgcca
    13741 gccaaaaacg ctgataatta gaaattaatg ataaacaggg agggatatca gtgaaaggca
    13801 tgagtcgatg aatgactgca tgccaataac atgtgacagg ttacgacgcc agagccagca
    13861 cctcagccac ccactgctgg aagcctttca catccagatc caatgccacc tgtacattgg
    13921 ctggcttgcc caggcaaccg tcgatatcaa ccaccgtcgt gcctgaggta aattcgccct
    13981 gagtttccac tgccacaaaa cagggtttga gagtgaacag gtccgggcgc accagccagg
    14041 cgatggcgca gagatcgtgc attcgcagac cgctttgcat actgccgcta cggtagtggc
    14101 taaacagtgc atgaagcatt tttccggtgc gattcaactc cggcaaagta gcgagatagt
    14161 caggggttaa tattgcctga ttggtgacat ccagaccgca catgacgatt tcgattccgc
    14221 tacggaagac acaggcagcc gcttctggat cggcagcgat attaaactcg gcgtttggcg
    14281 tacagttgcc gcgtccggca gaaccaccca tgatcaccag acggcgaata tacggcttgc
    14341 attccgggca ttgtgaaagt aacagcgcaa tattggttaa cgggccgatg gccaccaggg
    14401 taacaggctc tggtgcacgc atcagggcat cccgaatcgc cagaaacgcc ggtatcccga
    14461 gcggctttcg gttgtgctca acaaagtcgt agccagccat tcccgattcg ccgtgcacag
    14521 atgccgcatc acgcggtgcg cgtaccagcg gcacagcggc cccttgggcg agcggaatct
    14581 ccgcattcca gaaatgcagc agttgcaggg cattgcgggt agttttctca accgagacat
    14641 tacccgcgac ggtggtcatc agttgcaggt cgagttcggg tgcaaaaatc gcggcggcaa
    14701 tggcgacggc atcgtcaatg ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt
    14761 ctccataaaa aatgccggta acaagaccgg cattttcgca taacttaggc tgctaatgac
    14821 ttaatcgact tcacgaatat cgacacgcag ctctttcggc acttcgaaaa caatgttttc
    14881 ttcacggcct tccagcggaa tggcttcacc accgcccagc tgctgcaaac gtgccaccac
    14941 attctgcacc agaatatccg gagccgatgc gcccgcagtc acgccgacgc atttaacctc
    15001 tttcacccac tcttcctgga tgtctttcgc atcgtcaatc aaaaacgcgc gtttgcccat
    15061 acgctgggcc agctccgcca gacggttgga gttggaggag tttttcgaac cgaccaccaa
    15121 cacaacttcc gcctgttctg ccagggcgcg taccgcttcc tgacggttag tcgtggcgta
    15181 gcagatgtca tctttgcgcg gaccgacaat tttcgggaag cgtttacgca gcgcgtcgat
    15241 cacatcagac gtgtcatcca ccgacagcgt ggtctgggtc ataaaggaga gcttctcttc
    15301 gtttttgacc gtcagtttcc acacatcgtc cggcgattcg accagataca ttcccccttc
    15361 cgggttactg tactggccca ttgtcccttc cacttccggg tgcccggcgt gaccgatgag
    15421 aatagattct tcgccacggc gactggcgcg ggcgacttcc atatgcactt tggtcaccag
    15481 cggacaggtc gcgtcaaata ccgtcaaatc gcgacttttt gcttcgttac gtaccgcctg
    15541 agaaacaccg tgtgcggaga aaatcaggat cgcgccgtcc ggtacttcgc taatctgctc
    15601 aataaagata gccccacgct cacgcaagct atcgaccaca tagcggttat gtaccacttc
    15661 gtgacggaca tatatcggtg cgccgtaaat ggccagcgcg ttttcaacaa tgctgatagc
    15721 gcggtctacc ccggcacaaa aaccacgcgg gttggccaac aggatctgca tgttacgcct
    15781 ccagtgccgg atcgatttcc agcacttcaa tatcaaaatg aacggtctgc ccggccagcg
    15841 gatggttgaa atcaacggta atggagtcgc cgttaatttc gcggatcacg ccaggcatct
    15901 cactgccatc cattgcggta aaaagcatga ttgcgccaat ttctggctcg cctgcatcca
    15961 taaattcacg gcgggagaag tactgaatca ggtccggtga cggcacgcca aacgccgcat
    16021 ctggctccaa cgagaaggtg gttttatcgc ccactttcag ccccaacagg tgttgctcca
    16081 gcccttcaga aagagaagca tcacccaggc ggaacagcgc cggtttaccg ttgttgcggg
    16141 tagactcggc ggtggtgcca tcgtcgagtt ttagcgtgaa gtgcaccagg acggcgctat
    16201 tgctctgtac agattcagac atgcaggttt gctcttatat tttgaggatt tttgccggat
    16261 gcggcctacg atttcgcagc aatctgtagg ccttatccgg cagggtttat tattgttttt
    16321 tcgctttaga aggcaaaaaa ccttccagca caatcagtgc cgcaccgaca caaatggcag
    16381 tatcggcaag gttgaaggtg gcgaagtgcc agtcgccgac gtagaagtcg atcatatcga
    16441 caacgaagcc gtgccacagg cggtcgaaca ggttgcccag cgcgccgcca ataatcagcg
    16501 cataagcgat attgtttagc ttctgcgtgg ccttcgagcg atacatcatc actgccagga
    16561 tcacgctaat accaatcgca ataccggcaa agaaccaacg ctgccagccg ccgctatcgg
    16621 caaggaaact aaacgccgcg ccatagttac gcgcataatg cagattaagc gacgggaaca
    16681 gcgggaccgt atcccccaga gcaaagttct ggaggatcag gtatttgctg cccagatcga
    16741 taatcagcac gactaccacc agccacagcc agcgtagccc tgttgaacag atcgattgac
    16801 tcatcaggca aacttacgtt tttcaccgtc accggcgacg ttgctgacac agcggccgca
    16861 gatttctgcg tgttccgcca ccttgccgac atcctgggtg tagtgccagc agcgtgggca
    16921 cttctcacct tcggctttac tcaacgcgac tttcagccct ttgagtactt cgctctgctg
    16981 agcatcagca ggtgcgtcgt tatagtctgc aacggtagcg ccggaggtca acaggacaaa
    17041 tcgtaattca tcgcccagcg cggtcagttt cgccgacagt tccggttctg catacaaggt
    17101 tactgccgct tccagcgagc cacccacttt cttgtcggca cgcgcttgct caatgacttt
    17161 gttcacttcg ccacgcactt tcaacagctc gtcccagaac gcatcgttca tcgcttcact
    17221 gtctgccagg ccaaacaggc cttcgtacca ctcaccggtg aagacgtatt tttcacgttc
    17281 gcccggcagg tagccccaca cttcatcagc ggtgaaggag aggattggtg ccatccagcg
    17341 caccagcgct tctgcgatgt gatacagcgc agtctggcag ctacgacgcg ccacactgtc
    17401 cgctttggcg gtgtactgac ggtctttgat gatgtcgagg tagaaggaac ccatctcaac
    17461 ggagcagaag cgcatcagac gctgtaccac ttcgtggaaa tcgtatgctt cgtacgcctt
    17521 gaggatgtct tcctgtgccg ctttcgcaca acctacggcc cagcgatcca gtaccaccat
    17581 ctcttccggt ttcaccatat cttttgctgg atcaaaaccg ttcaggtttg ccagcaggaa
    17641 gcgcgcggtg ttacggatac gacgatagct atcggcagca cgtttcagga tctcgtcaga
    17701 aacggccatt tcaccggtgt agtcggttga tgccacccac agacgcagaa tatccgcgcc
    17761 cagtttgttc atcacatcct gcggcgaaac ggtattgccg atggatttag acatcttgcg
    17821 accctgacca tccacggtaa agccgtgggt cagtacctga cgatacggcg ctttgccttt
    17881 catcgcggtg gagatcatta gggaagacat gaaccaacca cggtgctggt cagaaccttc
    17941 cagatacatg tccgctgcgt gaccggcaaa ttccggacgc acgtcaacaa cagaagagtg
    18001 ggtagatccg gagtcaaacc atacatccaa tgtgtccggc actttcacgt actgatcagc
    18061 ttcatcgccg aggatctctt tcgcatcaag atcccaccac gcctggatgc cgtcaacttc
    18121 aacgcgtttt gccacttctt ccattagttc gagagtacgc ggatgcagct cttccgtgtc
    18181 tttgtgcacg aacagtgaca tcggtacgcc ccaggtgcgc tgacgggaga tacaccagtc
    18241 aggacggtta gcaaccatcg actcgatacg cgcctggccc cagtccggga tccactgcac
    18301 gcctttgatc tctttcagtg actgcgcacg cagacctttc tgatccatgc tgacgaacca
    18361 ctgcggcgtc gcgcggaaga tgatcggcgt tttgtgacgc cagcagcacg gatagctgtg
    18421 ctgcattttc tcaacgtgca gcagcgcgcc tttttcctgc agcagcgcaa cgacgatgtc
    18481 gttcgctttg aagacgttca cgccatccag cgtcggataa gtgcccggca gataagtgcc
    18541 gtccgggcca accgggttag cggtttccag gccgtatttc tgaccgatca catagtcgtc
    18601 cgggccgtgg ccaggcgcgg tgtgaacggc accggtaccg gcatccaggg taacgtgatc
    18661 gccgaggatt gccggaacgt cgaagcccat aaacggatgg gtaaagcgca gcaactcaag
    18721 ctccgcacct tttaccgtgc cgagaatggt gtaatcggtc acgccgatac gctgcattac
    18781 gctttcaacc agatctttcg ccagaatcac ggcctgaccg tcgatctgca ccagcgcata
    18841 gtcgaaatca ggtgcaatag agattgcgcg gttcgcaggc agagtccacg gcgtagtggt
    18901 ccagatcacc agcgagattg ggccgttaac gttgcttacg gcaaattttg ctttcagtgc
    18961 atcctgatcg accgcctgaa aagcaacgtc gatggacgga gaagttttgt cgtaatactc
    19021 aacttccgct tccgccagcg cagaacggca gtcaacgcac cagtgaactg gcttcgcgcc
    19081 tttgtgcagg tgaccgttgc cgatgatttt gcccagcgcg cggatgatgt tggcttcagt
    19141 tttgaagtcc atggtcaggt acgggtgcga ccagtcgccc agcacgccca gacggataaa
    19201 gtctttgcgt tgaccgtcaa cctgggtcgc cgcgtattcg cggcacttgg cgcggaactc
    19261 ggcggcggtg aatttctcac ccggcttacc gtattcttgc tctactttca gctcgatcgg
    19321 cagaccgtgg cagtcccagc caggcacata cggcgagtca taaccggaaa gccctttgga
    19381 cttcacgata atgtctttca gaatcttgtt aaccgagtga ccaatatgaa tgctgccatt
    19441 cgcataagga gggccatcat gcagaatgaa ggtttttttg ccttttttag ccgcacggat
    19501 gatgccgtac agatcatcat cagtccaacg cgccagcatt cccggttcgc gcttggcgag
    19561 atcgccacgc atcgggaacc ctgtttccgg caaattcagg gttgatttat agtcactcat
    19621 cagattctcg gttccgtgtt tcggtttgat tacataacag gcttaagccg gttttgttag
    19681 cccaaaaaat tcgcgggcgg ttaattcatc acgcgcaatc tgcgctttca gttcgtccag
    19741 cgacgcaaat cgctgctcat tgcgtatttt tttacgcagc actacttgta tatggcgacc
    19801 gtaaaggtcc attgcaacat ctaacaaatg cacttccagc tgctggcgaa taccggcaac
    19861 cgttgggcgt gttccaatgt ttgccacgcc gggtaacggc ttttcaccga ggcccagcac
    19921 ttctaccgca taaacccctt tcaccgggga aacctgacgg cgtagcggta cattcgccgt
    19981 cgggaaacct atagtgcgcc ctaattcatc accgtggact acacgcccgg agatggcaaa
    20041 cgggtgcccc agtaaacttt ctgccagagc cagattgtca tccgccagcg cctgacgcac
    20101 agcggtgctg ctgatacgca cgccaccttc gcaaaaagtt tgcgtactgg tgatatcgaa
    20161 gccgtattcc atgccagctt tctgtaataa caagaaatcg ccttcacgac cagcgccaaa
    20221 gcggaaatca tcacctacgg caagaaattt tacgcgcaaa tgcttcacca gaagatcgct
    20281 gatgaaattt tgcgcggtta acgccgcgaa acgcctgtcg aaacgcacgc acagcacgta
    20341 atcaacgcca cactctgcaa ggtaacgcag tttttcccgc agccgggtca gccgtgccgg
    20401 ggctttatcg gtagcaaaca gttccagtgg ttgaggttca aaaagcatca ccatcaccgg
    20461 taagttgcgc ttgcgccctt cttcctgcaa gccctgtaac agcgcgcgat gaccgcgatg
    20521 cacgccgtcg aaattaccaa tagtcagcac acacccttct tgcggggcct ggctgagatt
    20581 atgtatgccg cgtatcagct tcatgtctgg ctcaaaacag tgaaaatcgt ccgagtatac
    20641 cttgtacagc ggtcaaggtt aaccggcgat tgagtaccga aatcagaaag aagtggcttt
    20701 tcattgccat ggcgcaaatc acgggaagaa agtgaccgcc tgctgcaatt tttatcgcgg
    20761 aaaagctgta ttcacacccc gcaagctggt agaatcctgc gccatcacta cgtaacgagt
    20821 gccggcacat taacggcgct tatttgcaca aatccattga caaaagaagg ctaaaagggc
    20881 atattcctcg gcctttgaat tgtccatata gaacacattt gggagttgga ccttggctaa
    20941 tatcaaatca gctaagaagc gcgccattca gtctgaaaag gctcgtaagc acaacgcaag
    21001 ccgtcgctct atgatgcgta ctttcatcaa gaaagtatac gcagctatcg aagctggcga
    21061 caaagctgct gcacagaaag catttaacga aatgcaaccg atcgtggacc gtcaggctgc
    21121 taaaggtctg atccacaaaa acaaagctgc acgtcataag gctaacctga ctgcacagat
    21181 caacaaactg gcttaatcgc caatttgctg aagctttgtg aaaaagcccg cgcaagcggg
    21241 tttttttatg cctgctgctt ttatgatgcg tgagagaaca cactaccggt tgccagatag
    21301 tcaaaagatg ctggcttttg cagacatgcc gttacacttt ctgaactctt cgtggcttaa
    21361 gaatcgtaga accatcggct gatacattct gaacaagaat atctactcaa tctccagact
    21421 cccctacaaa gaatgtggat attgtgatac acattgaggt attaaactta agaccactct
    21481 atttttgaat taaattccta tacactttaa ccattcataa atatctcctt attagatctt
    21541 gtaaataatt aacaacaatc aacacaggtt aaatacctat ttatattcta cttcataaaa
    21601 ttaatgagct atttttgata gtagtcacat tcactcataa attaaattaa taaaaattca
    21661 tcaacttcaa ggactgataa tgacaaatgg tatctcaact tcgccacatt gtctttataa
    21721 atcaaatata gtagatgatg taataataaa taaaacaaga caaaatgagt tagtaaaagt
    21781 attttgcgag tataaaatag agttcttaat tttgtttgat gactttttta gaagccataa
    21841 cttgcctaaa ccatctccag tccttcatca tttttttcaa tatactcatc tgagagatgc
    21901 atatttttat agatgtaaac tcatagaaca tacagttcaa ttttattttt ttaaacacaa
    21961 aggtatcaca ttacttagat tggatgtttt tgatgacaga acaagcgaat gtctttctga
    22021 agagattaag atataccaag aatgccatga aaaattcgtt aattttttaa aggcaaactt
    22081 caaccaagaa atatacccac agctatatac tcctgaaata ttttatgagg cctgcagaaa
    22141 tctccagtca ttttatgatc atcaggaaac gtcacaaaac gcaaaataca gtgcaataga
    22201 caagaaaaaa agtcatctta accaggaaat caaaaagctt attaaaaaaa atattttccc
    22261 tgagctatac aatgagcaat gcaataaaat cccctcctca tcgatagatg ataaccaggg
    22321 aataatgtgg cagaacttta aaactagcaa tattgcatat tcacaactat gtaaaaaatt
    22381 atctttattt aaaagcatac ccagtcgatt aataaaaaaa tcagcttact gctcaactga
    22441 aaacacaatc accaataaat ttgatgttgt cttttcttac tgcggaaata atgtaaagga
    22501 atttatactt ttattaccct ataataaatc tctggagatg catgaactta acgagcaaaa
    22561 catccagtat ttgactgccc ttaatattaa tatacataaa atgttgctat caaatataac
    22621 aatagaaaag agcgaccttt catatgggta ttattttggt tgtgtattat ccaatatatt
    22681 atgttttgaa agcgacttat caaacaccat cttttcaaat ggagaaatta ataatctctt
    22741 cattaaaaaa agcaacatat ttggagcatc attcactaat accagaataa aaaatttgct
    22801 ctgtgaagat attatgcctg gtcgatggac cactcaactt gtcaataagc acttaggtta
    22861 cagatacaca ggagtattca aaaccctggc aagtattgat gacaaaccgt cccgtttcga
    22921 aattttgata ccattggtac aaactttagt tcgcgacaat gtaaagctaa acaatgatgt
    22981 gtataaagaa cttaataagt ttatgcacga ttacgacaaa acatcatcag aaatgagaaa
    23041 ataccttaaa agcatcaacg agtgtatgtt ccttatgaaa aacatagctc atcaaaatta
    23101 aaaaatctca aaaaagaagt aatttcagaa tgaaattaac tgtgatgatt attaattcac
    23161 tacagaggaa taaaaaataa tctgaagcat actctgaaac atcctactct ctcactctaa
    23221 taatggcacc ttgctcttct tataaagaag ggaataatta agaatattaa actttcatca
    23281 acttcacatc aagaaattaa aaattttaaa cattaatcta aataataaca acctgatcac
    23341 ataatcgggt acgattttat ccgataacta taatgctata actctgggaa atggttggaa
    23401 tacaccgctg ggtgccatct ctttttacgc tgcccgatcg agtagtaagt taaataatga
    23461 taccagacac gaaggtacga gctatcaggt cgccta
//
LOCUS       DAEVZA010000075        23131 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEVZA010000075 DAEVZA010000000
VERSION     DAEVZA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.075"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..776
                     /locus_tag="LAF92_002951"
     CDS             <1..776
                     /locus_tag="LAF92_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBI9791921.1"
                     /translation="LAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAF
                     GWLLPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRM
                     AQVLTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALT
                     PTRIALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLL
                     VFADWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(811..2091)
                     /gene="hemL"
                     /locus_tag="LAF92_002952"
     CDS             complement(811..2091)
                     /gene="hemL"
                     /locus_tag="LAF92_002952"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBI9791922.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            2316..3737
                     /gene="clcA"
                     /locus_tag="LAF92_002953"
     CDS             2316..3737
                     /gene="clcA"
                     /locus_tag="LAF92_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBI9791923.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            3761..3826
                     /locus_tag="LAF92_002954"
     CDS             3761..3826
                     /locus_tag="LAF92_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791924.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            3819..4163
                     /gene="erpA"
                     /locus_tag="LAF92_002955"
     CDS             3819..4163
                     /gene="erpA"
                     /locus_tag="LAF92_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBI9791925.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(4210..4833)
                     /locus_tag="LAF92_002956"
     CDS             complement(4210..4833)
                     /locus_tag="LAF92_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBI9791926.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(4871..5671)
                     /gene="btuF"
                     /locus_tag="LAF92_002957"
     CDS             complement(4871..5671)
                     /gene="btuF"
                     /locus_tag="LAF92_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBI9791927.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(5664..6362)
                     /gene="mtnN"
                     /locus_tag="LAF92_002958"
     CDS             complement(5664..6362)
                     /gene="mtnN"
                     /locus_tag="LAF92_002958"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBI9791928.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            6446..7963
                     /gene="dgt"
                     /locus_tag="LAF92_002959"
     CDS             6446..7963
                     /gene="dgt"
                     /locus_tag="LAF92_002959"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBI9791929.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            8093..9517
                     /gene="degP"
                     /locus_tag="LAF92_002960"
     CDS             8093..9517
                     /gene="degP"
                     /locus_tag="LAF92_002960"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBI9791930.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            9672..10829
                     /gene="cdaR"
                     /locus_tag="LAF92_002961"
     CDS             9672..10829
                     /gene="cdaR"
                     /locus_tag="LAF92_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HBI9791931.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(10918..11304)
                     /locus_tag="LAF92_002962"
     CDS             complement(10918..11304)
                     /locus_tag="LAF92_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBI9791932.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(11618..12442)
                     /gene="dapD"
                     /locus_tag="LAF92_002963"
     CDS             complement(11618..12442)
                     /gene="dapD"
                     /locus_tag="LAF92_002963"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBI9791933.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(12473..15145)
                     /gene="glnD"
                     /locus_tag="LAF92_002964"
     CDS             complement(12473..15145)
                     /gene="glnD"
                     /locus_tag="LAF92_002964"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBI9791934.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(15207..16001)
                     /gene="map"
                     /locus_tag="LAF92_002965"
     CDS             complement(15207..16001)
                     /gene="map"
                     /locus_tag="LAF92_002965"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBI9791935.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            16369..17094
                     /gene="rpsB"
                     /locus_tag="LAF92_002966"
     CDS             16369..17094
                     /gene="rpsB"
                     /locus_tag="LAF92_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBI9791936.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            17352..18203
                     /gene="tsf"
                     /locus_tag="LAF92_002967"
     CDS             17352..18203
                     /gene="tsf"
                     /locus_tag="LAF92_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBI9791937.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            18350..19075
                     /gene="pyrH"
                     /locus_tag="LAF92_002968"
     CDS             18350..19075
                     /gene="pyrH"
                     /locus_tag="LAF92_002968"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBI9791938.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            19367..19924
                     /gene="frr"
                     /locus_tag="LAF92_002969"
     CDS             19367..19924
                     /gene="frr"
                     /locus_tag="LAF92_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBI9791939.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            20016..21212
                     /gene="ispC"
                     /locus_tag="LAF92_002970"
     CDS             20016..21212
                     /gene="ispC"
                     /locus_tag="LAF92_002970"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBI9791940.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            21401..22159
                     /gene="ispU"
                     /locus_tag="LAF92_002971"
                     /gene_synonym="uppS"
     CDS             21401..22159
                     /gene="ispU"
                     /locus_tag="LAF92_002971"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBI9791941.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            22172..23029
                     /gene="cdsA"
                     /locus_tag="LAF92_002972"
     CDS             22172..23029
                     /gene="cdsA"
                     /locus_tag="LAF92_002972"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBI9791942.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
BASE COUNT         5531 a         5660 c         6255 g         5685 t
ORIGIN      
        1 tgctggcggt agcgggctgt attattcagc ggctgaccgg caacccgatg gcaagcccgg
       61 aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt gttaatgctg tttctggtac
      121 cgggtaatgc ctttggctgg ctgttacccg caggcagtct cggggcggcg gtgacgctgt
      181 tgatcattat gatcgccgcc ggacgcggtg gattttcccc acaccgcatg ttactggcgg
      241 ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat gttgcaggca agtggtgacc
      301 cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg accgatgcgc
      361 aggtctggcg caccggaatt gtgatggtga ttttgctggc gattaccccg ctgtgccgcc
      421 gctggctgac cattttaccg ctgggtggtg ataccgcccg agccgtagga atggcgctga
      481 cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc gcgacgatga
      541 ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg atgatgggct
      601 ttcgacggac gatgccacac atcgtaattt cggcgctggt gggtggttta ctgctggtgt
      661 tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccggcg gggctgctgt
      721 caacctttat cggcgcgcca tattttatct atttgttgag aaagcagagc cgttaattga
      781 atgcctcgcc cggtgggcga ggcaaaatca tcacaacttc gcaaacaccc gacgtgcagc
      841 atcgatggtg ttattgatat cttccatgct gtgcgccacg gacataaagc ccgcttcaaa
      901 cgctgacggt gccaggtaaa caccttcatc cagcatcata tggaagaaac gcttaaagcg
      961 ttccacgtca caggccatca catcctgata gcacgtcacg gactcggcgt cggtaaagaa
     1021 aataccgaac atgccgccaa cgtggttaac gaccagcgga attccggctt cttctgccgc
     1081 ttccagcaga ccttctgcca gacgtgatgt cagctcatcc aacgtttcgt gaacgcccgg
     1141 ttgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaattgggt taccggaaag
     1201 cgtacccgcc tgatagaccg gacccgtcgg ggccagcgca tccattacat cacgacgacc
     1261 accgaatgcg cctaccggca ttccaccgcc gatgattttg cccaggcagg tgagatccgg
     1321 ttccacaccg taataatcct gtgcgccagc tagcgctacg cggaagccag tcattacttc
     1381 atcgatgatc agcaatgcgc caaattcgtc gcacagcgcg cgcagacctg gcaggaactc
     1441 tggcagcggt ggaacgcagt tcatattgcc tgccaccggc tcgacgataa tacaggcaat
     1501 ctcttgcggg tattgctcaa acgcggcgcg tacagaagcc agatcgttat aagtacaggt
     1561 taaggtatgt ttggcgaaat ctgccggaac acccggcgag tttggctggc ctaacgtgag
     1621 tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac aaccttcaaa
     1681 tttaatgatt ttgtcgcgac cggtaaaacc acgggccagg cggatggcgc tcatggtcgc
     1741 ttcagtgccg gagttcacca tgcgcaccat atccatggtc gggaccagtt cggtcaccag
     1801 ttgcgccatt ttcacttcca tttcggttgg tgcaccaaag cttaaaccac gctcggcggc
     1861 ttcaatcacg gcattgcgga tcgccggatg gttatggccc agcaccatcg gcccccagga
     1921 accgacataa tcgatatagg ctttgccatc aacatcgtac agataggcgc cgtccgcttt
     1981 ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg agttcacacc
     2041 gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca tggagggttc
     2101 ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa gttaaatact
     2161 cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta taaaagtttt
     2221 gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag taaaatgccg
     2281 tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact ccctctttag
     2341 aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt cttgagcgcg
     2401 ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt gtcgggctgg
     2461 cagcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg ggggcgctgg
     2521 tacatactgc tgataattat ccgctactgt taaccgtcgc ttttctctgt tcggcggtgc
     2581 tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc cgaagcaggg ggatcgggga
     2641 tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt tcgctggtgg cgtgtattgc
     2701 cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggtgcta gggcgcgaag
     2761 gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt gcttgatatt ttccgcctga
     2821 aaggtgatga agctcgccat acgctgctgg caaccggtgc tgctgcgggg ctggctgcgg
     2881 cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt ccgcagtttc
     2941 gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg accattatgt
     3001 accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct gatgcgccgc
     3061 ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc ggccctattt
     3121 ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta
     3181 ccaaatgggt actgatgggt ggtgcgattg gcggtctgtg tggattgctg gggtttgtgg
     3241 caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg gggaatttca
     3301 gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta ctctgcttct
     3361 cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact gtgctgggaa
     3421 ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag gcggggacgt
     3481 ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg ttaacgggga
     3541 tcattctggt tctggagatg accgataact accagctcat tttgccaatg attattaccg
     3601 gtcttggcgc aacactatta gcgcaattta ccggcgggaa accgctatac tcggcgattc
     3661 ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag gctgcatcag
     3721 ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta ttattgggtt
     3781 agaatttgcc caattgccga tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc
     3841 cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct gacgaagata
     3901 acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc ttccagtatg
     3961 gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg
     4021 gcctggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt gattataccg
     4081 aaggtctgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt
     4141 gcggttcttc ctttagtatc taatctgttg ttcatgtgcc ggataaactt tccggcacat
     4201 cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg
     4261 aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg ctgtttccag
     4321 tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa tacaggcagt
     4381 tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg gccagaacat cacgaatgat
     4441 cccgccgcca acgccagtaa tgacgcccat acaaaccgcg attaacggac cggcttccgc
     4501 attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg cgtccagcac
     4561 cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg tcagcatgct
     4621 ggttaccatt gcaacgacca gatcggtggg atctttcacc caaaataccg ggccgtgatc
     4681 cagcgccatg tcgcgaattg tcccgccgcc tacagcggta accacgccca gtaccagaac
     4741 accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg caaataccgc
     4801 tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga gcacctgaaa
     4861 aggtcaggca ctaatctacc tgtgaaagcg cattacagag ctgttgtgca gcgaggataa
     4921 tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga attttgagct
     4981 gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta atgacaatcg
     5041 cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga acccggctgt
     5101 ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc gactcttttc
     5161 cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta ggtttatcag
     5221 catattgcgc tttcaattgc gcgtactgat ccagcaggga ttgcgcggct tgttcggcct
     5281 tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt tgttcaatgc
     5341 ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca acctgccgct
     5401 cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca atgcgttcca
     5461 gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga ggatagtcgg
     5521 aatagctgct gaccccaacc ggcgtgatcc cggcagcaaa ggcaagttca gtgttggcgg
     5581 gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcaaga aaagacaggg
     5641 cgaccagcgc cctgaacagt gacttagcca tgtgcaagtt tctgcaccag tgactcaacc
     5701 atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag atgagactgt
     5761 tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa attgtggcag
     5821 acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa gttgtggcgg
     5881 attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat caggccacgt
     5941 acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca
     6001 gctttaaagc ctgccggaca gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca
     6061 tccgcgtcgt gataacgtgc ttcgtccgag acaacgatat cgcccacttt cagcgttggt
     6121 gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg ttccaacagc
     6181 agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag cgcaacctcg
     6241 gttccattca gttggccggt atagatttcg caaccgccga gactgatagt ttgacggttt
     6301 tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat gccgattttc
     6361 atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc ttacggggaa
     6421 gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa aaataaactg
     6481 gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc tgcggatctt
     6541 cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac
     6601 ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttactc actcgatgga
     6661 agtccagcag gtggggcgct acatcgccaa agaaatttta agccgtctga aagagcttaa
     6721 attgctggaa gcatacggcc tggatgaact gaccggacct tttgaaagca ttgttgagat
     6781 gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg aagcagcgat
     6841 aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc ctctgactga
     6901 cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc ttaacgagct
     6961 gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag gcattcgtct
     7021 ggtgcataca ttgatgcgga tgaatctcac ctgggcacag gttggcggta ttttaaaata
     7081 tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt taatgaaaaa
     7141 gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag aacttaattt
     7201 ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg acatctccta
     7261 ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg agcagcttta
     7321 tcatcatttg cacgaagcgt ggggccagca tgagaaaggt tcgctctttt cgctggtggt
     7381 tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg aagatcagtt
     7441 ttttatgtat ttacgagtaa ataccctaaa taaactggta ccctatgcgg cacaacgatt
     7501 tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat tggaagatgc
     7561 cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac atgtgtttag
     7621 ccatccagat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg gattattaga
     7681 gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg tagaaaaaga
     7741 acgggtgaaa cgtttcccta ttgaatcgcg cttattccac aaactctcga cgcgccatcg
     7801 gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt ttccgctatg
     7861 ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga ccgacctcta
     7921 tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccaggct tttgtaaaga
     7981 cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcgcg ctataaaacg
     8041 aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat acatgaaaaa
     8101 aaccacatta gcactgagtg cactggctct gagtttaggt ttggcgttat ctccgctctc
     8161 tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa gccttgcacc
     8221 gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta gcacaaccgt
     8281 taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt ctccgttctg
     8341 ccaggaaggt tctccgttcc agagctctcc gttctgccag ggtggccagg gcggtaatgg
     8401 tggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg atgccgataa
     8461 aggctatgtc gtcaccaaca accatgttgt tgataacgcg acggtgatta aagtccagct
     8521 gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct ctgatatcgc
     8581 gctgatccaa atccagaacc cgaaaaacct gaccgcaatt aagatggcgg attctgatgc
     8641 actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggtctgg gagagacggt
     8701 aacttccggg attgtctctg cgctggggcg tagcggcctg aatgccgaaa actacgaaaa
     8761 cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc tggttaacct
     8821 gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg gcaacatcgg
     8881 tatcggtttt gctatcccga gcaacatggt gaaaaacctg acctcgcaga tggtggaata
     8941 cggccaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt ccgaactggc
     9001 gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc tgcctaattc
     9061 ctccgctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga acggtaagcc
     9121 aatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg gcagcaaact
     9181 gaccctgggc ttgctgcgcg acggtaagca ggtcaacgtg aatctggaac tgcagcagag
     9241 cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggcg ctgagatgag
     9301 caacaaaggc aaagatcagg gcgtggtagt gaacaacgtg aaaacgggca ctccggctgc
     9361 gcagatcggc ctgaagaaag gtgatgtgat tattggcgcg aaccagcagg cagtgaaaaa
     9421 catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac tgaacattca
     9481 gcgcggcgac agctccatct acctgttaat gcagtaatct ccctcaaccc cttcctgaaa
     9541 acgggaaggg gttctcctta caatctgtga acttcaccac aactccatac atcttcatca
     9601 tcctttaggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa gccgtgcata
     9661 acggaggact tatggctggc tggcatcttg ataccaaaat ggcgcaggat atcgtggcac
     9721 gtaccatgcg catcatcgat accaatatca acgtaatgga tgcccgtggg cgaattatcg
     9781 gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg gtactttcac
     9841 agggacgagt cgtcgatatc gatgacgcgg tggcacgtca tctgcacggt gtgcggcaag
     9901 ggattaatct accgttacgg ctggaaggtg aaattgtcgg cgtaattggc ctgacaggtg
     9961 aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa atgatgctgg
    10021 aacagtcgcg gttgatgcac ttgttggctc aggatagccg tttgcgggag gaactggtga
    10081 tgaacctgat tcaggcagag gagaatactc ccgcacttac tgaatgggcg caacggctgg
    10141 ggatcgatct caatcaaccg cgagtggtgg ctattgttga ggtcgacagc ggtcagcttg
    10201 gtgtggacag cgcaatggcg gagttacaac aactgcaaaa cgcgctgact acgcccgagc
    10261 gtaataatct ggtggcgatt gtctcgctaa ccgaaatggt ggtgttgaaa ccggcgttga
    10321 actcttttgg gcgctgggat gcagaagatc atcgtaagcg agttgaacaa ctgattaccc
    10381 gcatgaaaga gtacggccag ctgcgttttc gcgtttcact gggcaactat tttaccggtc
    10441 ctggcagtat tgcccgatcc tatcgtacgg cgaaaacgac gatggtggtg ggtaaacagc
    10501 ggatgccaga aagtcgctgc tatttttatc aggatctgat gttacctgtg ttactcgaca
    10561 gtttgcgtgg cgactggcag gccaacgaac tggcgcgacc gctggcgcgg ctgaaagcga
    10621 tggacaataa tggcttgctg cgacgaacgc tggcggcgtg gtttcgccac aatgtgcaac
    10681 cgctggctac gtcaaaggcg ctgtttattc atcgtaatac tctggagtat cggcttaatc
    10741 gtatatcgga actgaccggg cttgatttgg gtaattttga tgacaggttg ctgctgtatg
    10801 tggcgttgca gctggatgaa gagcggtagg ttatgcgtta aggtcgaggc ggcgctggct
    10861 cattcccctc agcccgaagg gtagagggga atgtccgagt taaccgtcag ttcggcatta
    10921 tttacgcgtt agtttttcca gatcggcttc aatctcgctg atcttattgg ttacgactga
    10981 ctccaggtga cgtaagtcgt cgaggatctt acgcttaaga tcaacttcgc tgcggtcacg
    11041 ctggcagatt tgatcaagct catcaatgat ataccgtaga ttcgggctga tttcctggac
    11101 ttctttataa ccctgaccca caccatcagc gacgaccgtt ttacgctgac gcggatattt
    11161 aaacttaacg ctcttggcga aaaactcgcc tttgtctttc tggaaataga ttttcagaat
    11221 atcgttgttg gcttcctgcc ggaggctgta acgatcaatt tcttcaggat tggtaatacc
    11281 cagacttttc agattgtcgt acatagcgtt acctcaaaat gagtcagtaa attgtgctta
    11341 ttttagcatt tggcctggcc cgcccggctc gactgtgatc gcagaaagcc tttttcttcc
    11401 agacttctat catggcgtaa aaatcaaaaa attacctgct ttattgtggc gatattccat
    11461 gggatggatt agcacattta tcttcgtttt gcgaattaca tgacagtccg aaacaagatg
    11521 aaaaagtatt taaaaaaggg ggaggaagaa tgagtaggtc gaattattct gaaccagaaa
    11581 gcaatcgggc agcgtcgtgc tgcccgtgtg catactttta gtcgatggta cgcagcagtt
    11641 cgttaatgcc gactttgccg cgagttttcg cgtcaacttt cttaacgata accgcacagt
    11701 agaggctgta tttgccatct ttcgacggca gattacctga aacaaccaca gaccccgccg
    11761 gaacgcgacc gtagtggatt tcgccggttt cacggtcgta aatacgggtg ctctgaccaa
    11821 tgtatacgcc catagagatg acggaacctt cttcgacaat caccccttca accacttcgg
    11881 agcgtgcgcc aatgaagcag ttatcttcaa taatggtcgg gttagcctgc agcggctcca
    11941 gcacgccgcc gatgcccacg ccaccggaaa ggtggacgtt tttaccaatc tgcgcacaag
    12001 aaccgacggt cgcccaggta tcaaccatcg tgccttcatc aacatatgcg ccgatgttga
    12061 cgtaagacgg catcagcacg gtgttacggg caataaatgc accctgacgt accgccgctg
    12121 gtggcacaac gcggaagcct tctttctgaa aacgcgcttc gtcgtagtcg gcgaatttca
    12181 tcggcacttt atcgaagtag cggctttctg ccccttcgat cacctgatta tcattaatac
    12241 ggaaagagag cagcaccgct tttttcaacc actgatgcgt cacccactga ccgtcaattt
    12301 tttccgctac acgcagtgcg ccggaatcca gcagggcgat cacctgattt accgcttcgc
    12361 gggttacggt gtctgcattg gctggcgtga tctcggcacg gcgttcaaaa gcggtttcaa
    12421 taatgttctg taactgctgc attgttaaac tcttttcata tcagtaaaca catcaccctt
    12481 tatcgtttgg attgagggcc tctgtcaacc gctgatgcac ttcctgctgc aactcgttat
    12541 taagtgcacg ccggtcagcg gtggcaatta tgaataaatc ttctactcgc tcgccaatgg
    12601 ttgtaattcg ggcaccatga agcgaaattc ccagatcggc aaaaattttc ccgactctcg
    12661 ccagcagtcc tggctggtcg agcgcgataa gctcaaggaa cgatttgcgg tcggtatggg
    12721 tcggcaaaaa cgttacttcg gtttcaacag taaaatggcg taatttggcg ggttggcgac
    12781 ggggctgcgg tggctgccag ctactttgcg tcagtacttg ctccagacca aaccgaataa
    12841 cctcatgacg atctgcggac agcgggctgc catcgggttc cagcacgata aaggtatcca
    12901 tcgccatacc gtcgcgagtg gtgaaaattt gtgcgtcgtg aacacttaaa ttgcggcggt
    12961 ctaattcggc acagacggcg gcaaacagat aggggcggtc cgggctccag ataaaaatct
    13021 cggtgcctcc acgcgtagcc tgcgggctaa gcaataccag cggtttgctt aaatcatgct
    13081 gtaataaatg gcgggcatgc caggccagtt gatttgggct atggcggaca aaatagttag
    13141 cacgacagcg tgaccaaatt tggtgcagcg cctcttcgtc gatgttatcc atgcgcagta
    13201 gtgccagtgc ctggagttgg tgatggcgaa cccgttcgcg catatccggc gtgttttgca
    13261 ttccgcgtcg tagctgcttt tcggtggcaa aatagagttc gcgcaacaga ctttgcttcc
    13321 agctattcca cagcgtttcg ttggtggcgc aaatgtcagc cactgtcagg cataccagat
    13381 agcgcagacg attttccgtt tgaacttctt cggcaaactg cttgatgact tcagggtcct
    13441 gaatatcgcg gcgttgtgct gtcaccgaca tcagcaagtg ctggcgaacc agccaggcaa
    13501 ccagctgtgt ttcgcgtgag ttcagcccgt ggagttctgc aaaatgcact acatcctgag
    13561 caccgagaat ggagtggtcg ccgccgcgtc ctttggcgat atcgtgaaac agcgcggcga
    13621 tgaaaatcag ctcagttgac ggcaggcgcg gccagacgtc cacacacaac ggatggcgct
    13681 ggcgcgtttc ttcactggca aaactctcca gtttcagcat cacgcggata gtatgttcat
    13741 ccaccgtgta ggcgtggaac agatcaaact gcatctgccc gacgatatgc gaccattgcg
    13801 gcatatacgc gccgagcacg ctatggcgat gcattggcaa tagcccgcgc cgcaccgctc
    13861 cggggtgacg cagaatgctc aaaaacagtt ttcgtgcttc cggaatatta cacagcggtt
    13921 gttgcagatg gcgacgggca tggcgtaact ggcgcagcgt ggtggagtaa atgccggtga
    13981 tcgcactgtt gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc tggcgcataa
    14041 atagtgtttc atcacgcagg tcgattagcg taccgcgtag ctgaaactca tcgtcgattg
    14101 gacgtggttt ttcgtcggcg ggaagggcga ggatggcttc atcgaacagt tgcagcagca
    14161 tctggttgag ttcactgacg cggcgtgtaa cgcggaagta atccttcatc atccgctcga
    14221 ccggttcgtt gccttcacca ctgtaattca gacgctgagc gacgctaagt tggcgatcga
    14281 ataacagacg attatcgtaa cggctgacga ccagatgcag ggcaaagcga atacgccaca
    14341 gtatatgcag acattcgttt aattccgccc gctccgctga ggttaagaag ccaaacccaa
    14401 ccatttcatc cagcgatgtt gcgccaaaat gacggcgggc cacccattgc agagtgtgga
    14461 tatcgcgcaa gccgccaggg ctacttttga tgtctggttc aagattgtag ctggtgccgt
    14521 ggtaacgctg atggcgctgg ttctgttctt caactttcgc cgcgtagaac ttgtcggaag
    14581 gccagaatcc ttcgctaaaa atatgttttt gcagttcgag gaagagcgca acatcgccaa
    14641 ttaataagcg ggattcgatt aaattggtgg cgacggttaa atccgataac ccttccagca
    14701 tgcactcttc aagcgtgcgc acactatgac cgacttccag ctttacatcc cagagcagcg
    14761 ttaacagctc gcccactttt tgcgcctgat cgtccgggag ctttttacgg cttaaaatca
    14821 gtaaatcgat gtcagaaagt ggatgcagct cgccacgacc gtagccaccg acggcgacca
    14881 atgccaggtc ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc aggagctggt
    14941 cgataaactc ggtgcgcgcc tcaatcaact gttctgcaga gatcccattg tcaaaggcat
    15001 cacccagcca acgctggaaa gtatcgatat gggcttttat cccaccgacg gttaattcat
    15061 cacggggcca gacgcatgga ttttgcggtt gaccgggcag ggtggggaga gcggtgtttg
    15121 cgtactgttc tggaagggta ttcattgtgc gccacccata agattaaatt atcgcattaa
    15181 aaaagccggc attcgccggc ttcatcttat tcgtcgtgcg agattatcgc cgggatggtg
    15241 tcatccttgc gtagcgtcaa aatttcgcag ccgttatcag tcaccacaat agtatgctca
    15301 tattgtgcag acaagctgcg atctttggtt tttaccgtcc agccatcttt catggtgcgg
    15361 atctcttttt tacccgcgtt gaccattggc tcaatggtga acgtcatccc cggtttcagc
    15421 acgacgttgg tttcacggga gtcatagtgc agcacctgcg gttcttcatg gaagccgcga
    15481 ccaataccgt gtccacaata ttcacgaacg acggagaagc cttctgcttc gacaaacttc
    15541 tgaatcgccg caccgatttc gcgcaggtta atgcctggtt tgaccatgcg cagcgccagg
    15601 tacaggcttt cttgcgtgat gcggcacaga cgttcgccca tgatggtcgg cttaccgacg
    15661 ataaacattt tcgaggtatc gccgtggaaa ccatctttga ttacggtgac atcaatgtta
    15721 acgatatcgc catctttcag cagcttagca tcgtccggga taccgtggca caccacttca
    15781 ttaatagaga tgcaaacgga tttcggatag ccgtgatagc cgaggcaggc agaaaccgcg
    15841 tgttgttcat taacaatgta atcattacag atgcgatcca gctcgccggt gctgacgccc
    15901 ggtttaacat acggttcgat catctccagc acttcggcag ccagtcggcc agcgacgcgc
    15961 attttttcga tatcttctgg ggtcttgatt gagatagcca ttaattctgt ccatcagcgt
    16021 cggtgatacc gacaatatat atgtaagtgc cgtcaatggt atcacacccg ggcaaattga
    16081 gaatcattct gaatttcgcc aaacgtgcca ctgaaggttt tctataatag aaaattcgac
    16141 atctgatgct gtacacagcg ccaacaatta ttggtgtcca tgacgtattt gtggtataaa
    16201 gcgcgccgga cttccgatcc atttcgtata cacagactgg acggaagcga caatctcact
    16261 ttgtgtaaca acacacacgt atcggcacat attccggggt gccctttggg gtcggtaata
    16321 tgggatacgt ggaggcataa ccccaacttt tatatagagg ttttaatcat ggcaactgtt
    16381 tccatgcgcg acatgctcaa ggctggtgtt cacttcggtc accagacccg ttactggaac
    16441 ccgaaaatga agccgttcat cttcggtgcg cgtaacaaag ttcacatcat caaccttgag
    16501 aaaactgtac cgatgttcaa cgaagctctg gctgaactga acaagattgc ttctcgcaaa
    16561 ggtaaaatcc ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa agacgctgct
    16621 ctgagctgcg accagttctt cgtgaaccat cgctggctgg gcggtatgct gactaactgg
    16681 aaaaccgttc gtcagtccat caaacgtctg aaagacctgg aaactcagtc tcaggacggt
    16741 actttcgaaa agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct ggagaaactg
    16801 gaaaacagct tgggcggtat caaagacatg ggcggtctgc cggacgctct gtttgtaatc
    16861 gatgctgacc acgaacacat tgctatcaaa gaagcaaaca acctgggtat tccggtattt
    16921 gctatcgttg ataccaactc tgatccggac ggtgttgact tcgttatccc gggtaacgac
    16981 gacgcaatcc gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt acgtgaaggc
    17041 cgttctcagg atctggcttc ccaggcggaa gaaagcttcg tagaagctga gtaataaggc
    17101 ttgataactc ccccaaaata gttcgagttg cagaaaggcg gcaagctcga gaattcccgg
    17161 gagcttacat cagtaagtga ccgggatgag cgagcgaaga taacgcatct gcggcgcgaa
    17221 atatgaaggg ggagagccct tatagaccag gtagtacacg tttggttagg gggcctgcat
    17281 atggccccct ttttcacttt tatatctgtg cggtttaatg ccgggcagat cacatctccg
    17341 aggattttag aatggctgaa attaccgcat ccctggtaaa agagctgcgt gagcgtactg
    17401 gcgcaggcat gatggattgc aaaaaagcac tgactgaagc taacggcgac atcgagctgg
    17461 caatcgaaaa catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca ggcaacgttg
    17521 ctgctgacgg cgtgatcaaa accaaaatcg acggcaacta cggcatcatt ctggaagtta
    17581 actgccagac tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca gacaaagttc
    17641 tggacgcagc tgttgctggc aaaatcactg acgttgaagt tctgaaagca cagttcgaag
    17701 aagaacgtgt tgcgctggta gcgaaaattg gtgaaaacat caacattcgc cgcgttgctg
    17761 cgctggaagg cgacgttctg ggttcttatc agcacggtgc gcgtatcggc gttctggttg
    17821 ctgctaaagg cgctgacgaa gagctggtta aacacatcgc tatgcacgtt gctgcaagca
    17881 agccagaatt catcaaacca gaagacgtat ccgctgaagt ggtagaaaaa gaataccagg
    17941 tacagctgga tatcgcgatg cagtctggta agccgaaaga aatcgcagag aaaatggttg
    18001 aaggccgcat gaagaaattc accggcgaag tttctctgac cggtcagccg ttcgttatgg
    18061 aaccaagcaa aactgttggt cagctgctga aagagcataa cgctgaagtg actggcttca
    18121 tccgcttcga agtgggtgaa ggcatcgaga aagttgagac tgactttgca gcagaagttg
    18181 ctgcgatgtc caagcagtct taattatcga aaaggagccg cctgagggcg gcttcttttt
    18241 gtgcccatct tgtaaattca gctaaccctt gtggggctgc gctgaaaagc gacgtacaat
    18301 gtcgctagta ttaattcatt tcaatcgttg acagtctcag gaaagaaaca tggctaccaa
    18361 tgcaaaaccc gtctataaac gcattctgct taagttgagt ggcgaagctc tgcagggcac
    18421 tgaaggcttc ggtattgatg caagcatact ggatcgtatg gctcaggaaa tcaaagaact
    18481 ggttgaactg ggtattcagg ttggtgtggt gattggtggg ggtaacctgt tccgtggcgc
    18541 tggtctggcg aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga tgctggcgac
    18601 cgtaatgaac ggcctggcaa tgcgtgatgc actgcaccgc gcctatgtga acgctcgtct
    18661 gatgtccgct attccattga atggcgtgtg cgacagctac agctgggcag aagctatcag
    18721 cctgttgcgc aacaaccgtg tggtgatcct ctccgccggt acaggtaacc cgttctttac
    18781 caccgactca gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg tgctgaaagc
    18841 aaccaaagtt gacggcgtgt ttaccgctga tccggcgaaa gatccaaccg caaccatgta
    18901 cgagcaactg acttacagcg aagtgctgga aaaagagctg aaagtcatgg acctggcggc
    18961 cttcacgctg gctcgtgacc ataaattacc gattcgtgtt ttcaacatga acaaaccggg
    19021 tgcgctgcgc cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg aataattccc
    19081 gtgatggata aataagggta agattccgca taagtatcgc gggggcgtaa gtctggttat
    19141 aaggcgttat tgttgcaggc agtttggtca cggccagcgc gcagcaaccg gtgcgtacac
    19201 aagtacgtga ggatggcgag cactacccgg ggccaaaatg gcaaataaaa tagcctaata
    19261 atccagacga ttacccgtaa tatgtttaat cagggctata cttagcacac ttccactgtg
    19321 tgtgactgtc tggtctgact gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat
    19381 cagaaaagat gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa cccaaatcag
    19441 caaaatacgc acgggtcgtg cttctcccag cctgctggat ggcattgtcg tggaatatta
    19501 cggcacgccg acgccgctgc gtcagctggc aagcgtaacg gtagaagatt cccgtactct
    19561 gaaaatcaac gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga ttatggcgtc
    19621 cgatcttggc ctgaacccga actctgcggg tagcgacatc cgtgttccgc tgccgccgct
    19681 gacggaagaa cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg
    19741 tgttgcagta cgtaacgtgc gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga
    19801 taaagagatc agcgaagacg acgatcgccg ttctcaggac gatgtacaga aactgactga
    19861 tgctgcaatc aagaaaattg aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt
    19921 ctgatttctt gaacgacaaa aacgccgctc agtagatcct tgcggatcgg ctggcggcgt
    19981 tttgcttttt attctgtctc aactctggat gtttcatgaa gcaactcacc attctgggct
    20041 cgaccggctc gattggttgc agcacgctgg acgtggtgcg ccataatccc gaacacttcc
    20101 gcgtagttgc gctggtggca ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat
    20161 tctctccccg ctatgccgta atggacgatg aagcgagtgc gaaacttctt aaaacgatgc
    20221 tacagcaaca gggtagccgc accgaagtct taagtgggca acaagccgct tgcgatatgg
    20281 cagcgcttga ggatgttgat caggtgatgg cagccattgt tggcgctgct gggctgttac
    20341 ctacgcttgc tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa gaatcactgg
    20401 ttacctgcgg acgtctgttt atggacgccg taaagcagag caaagcgcaa ttgttaccgg
    20461 tcgatagcga acataacgcc atttttcaga gtttaccgca acctatccag cataatctgg
    20521 gatacgctga ccttgagcaa aatggcgtgg tgtccatttt acttaccggg tctggtggcc
    20581 ctttccgtga gacgccattg cgcgatttgg caacaatgac gccggatcaa gcctgccgtc
    20641 atccgaactg gtcgatgggg cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag
    20701 gtctggaata cattgaagcg cgttggctgt ttaacgccag cgccagccag atggaagtgc
    20761 tgattcaccc gcagtcagtg attcactcaa tggtgcgcta tcaggacggc agtgttctgg
    20821 cgcagctggg ggaaccggat atgcgtacgc caattgccca caccatggca tggccgaatc
    20881 gcgtgaactc tggcgtgaag ccgctcgatt tttgcaaact aagtgcgttg acatttgccg
    20941 caccggatta tgatcgttat ccatgcctga aactggcgat ggaggcgttc gaacaaggtc
    21001 aggcagcgac gacagcattg aatgccgcaa acgaaatcac cgttgctgct tttcttgcgc
    21061 aacaaatccg ctttacggat atcgctgcgt tgaatttatc cgtactggaa aaaatggata
    21121 tgcgcgaacc acaatgtgtg gacgatgtgt tatctgttga tgcgaacgcg cgtgaagtcg
    21181 ccagaaaaga ggtgatgcgt ctcgcaagct gaggataatc cggctacaga gagtcgcgct
    21241 atttgttagc gtagggcttc agtgatatag tctgcgccat ctgatcgtaa gtagttggct
    21301 ttataaggtc aggtatgccg tggttttaca cggctttttt ttgtataggc ttcagtattc
    21361 ctgagtaccg taaaccctgt cagggaataa aaaacgcgtg atgttgtctg ctactcaacc
    21421 acttagcgaa aaattgccag cgcatggctg ccgtcatgtt gcgatcatta tggacggcaa
    21481 tggtcgctgg gcaaaaaagc aagggaagat tcgtgccttt gggcataaag ccggggcaaa
    21541 atccgtccgc cgggctgtct cttttgcggc caacaacggt attgaggcgt taacgctgta
    21601 tgcctttagt agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact
    21661 gtttgtgtgg gcgctcgata gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg
    21721 tattattggc gataccagtc gctttaactc gcgtttgcaa gaacgtattc gtaaatctga
    21781 agcgctaaca gccgggaata ccggtctgac gctgaatatt gcggcgaact acggtggacg
    21841 ttgggatata gtccagggag tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca
    21901 accagatcag atagatgaag agatgctaaa ccagcatgtc tgtatgcatg aactggcccc
    21961 tgtagattta gtaattagga ctggggggga gcatcgcatt agtaactttt tgctttggca
    22021 aattgcctat gccgaacttt actttacaga tgttctctgg cccgatttcg atgaacaaga
    22081 ctttgaaggg gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc
    22141 cggtgatgaa acagcctgat gggggtcgct tttgctgaag tatcgcctga tatctgcttt
    22201 tgtgttaata cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat
    22261 cgtaacgctg gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac
    22321 cactcgttcg cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgctgatgct
    22381 ttttctgttg ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg
    22441 ggcttcgctg ggttggtgga ttgtcgcgct attgctggtg ctgttttacc caggttccgc
    22501 agcaatctgg cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc
    22561 cttcttctgg ggcatgctgg cgttacgggc ctggcactac gacgagaatc attacagtgg
    22621 cgcaatatgg ctgctctatg tcatgatcct ggtatggggc gctgactctg gcgcatatat
    22681 gtttggcaaa ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg
    22741 gcaaggcttt ataggtggac tagcgaccgc agcggtaatc tcatggggtt atggcatgtg
    22801 ggcgaatctc gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc
    22861 ctcagtgctc ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag
    22921 cggtcattta attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc
    22981 ggtaccggtc tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat
    23041 atgctgagtt ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcacc
    23101 gtgcatgaat ttggtcattt ctgggttgcc c
//
LOCUS       DAEVZA010000076        23112 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEVZA010000076 DAEVZA010000000
VERSION     DAEVZA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.076"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..2609)
                     /locus_tag="LAF92_002973"
     CDS             complement(<1..2609)
                     /locus_tag="LAF92_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI9791943.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELNETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGQLQVSWGKDK
                     NSNCIVEYKLPEVS"
     gene            complement(2651..3361)
                     /locus_tag="LAF92_002974"
     CDS             complement(2651..3361)
                     /locus_tag="LAF92_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI9791944.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENEGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(3722..4285)
                     /gene="fimA"
                     /locus_tag="LAF92_002975"
     CDS             complement(3722..4285)
                     /gene="fimA"
                     /locus_tag="LAF92_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBI9791945.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(4717..4925)
                     /locus_tag="LAF92_002976"
                     /pseudo
     CDS             complement(4717..4925)
                     /locus_tag="LAF92_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(5237..6559)
                     /gene="hipA"
                     /locus_tag="LAF92_002977"
     CDS             complement(5237..6559)
                     /gene="hipA"
                     /locus_tag="LAF92_002977"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HBI9791946.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHYLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            complement(6559..6825)
                     /gene="hipB"
                     /locus_tag="LAF92_002978"
     CDS             complement(6559..6825)
                     /gene="hipB"
                     /locus_tag="LAF92_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HBI9791947.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(7034..12454)
                     /locus_tag="LAF92_002979"
     CDS             complement(7034..12454)
                     /locus_tag="LAF92_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HBI9791948.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGI
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGKGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYIDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEE
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            complement(12985..14577)
                     /gene="lsrK"
                     /locus_tag="LAF92_002980"
     CDS             complement(12985..14577)
                     /gene="lsrK"
                     /locus_tag="LAF92_002980"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HBI9791949.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(14656..15609)
                     /gene="lsrR"
                     /locus_tag="LAF92_002981"
     CDS             complement(14656..15609)
                     /gene="lsrR"
                     /locus_tag="LAF92_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBI9791950.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            15858..17393
                     /gene="lsrA"
                     /locus_tag="LAF92_002982"
     CDS             15858..17393
                     /gene="lsrA"
                     /locus_tag="LAF92_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBI9791951.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            17387..18415
                     /gene="lsrC"
                     /locus_tag="LAF92_002983"
     CDS             17387..18415
                     /gene="lsrC"
                     /locus_tag="LAF92_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBI9791952.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            18415..19407
                     /gene="lsrD"
                     /locus_tag="LAF92_002984"
     CDS             18415..19407
                     /gene="lsrD"
                     /locus_tag="LAF92_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBI9791953.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGTINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            19419..20441
                     /gene="lsrB"
                     /locus_tag="LAF92_002985"
     CDS             19419..20441
                     /gene="lsrB"
                     /locus_tag="LAF92_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HBI9791954.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            20468..21343
                     /gene="lsrF"
                     /locus_tag="LAF92_002986"
     CDS             20468..21343
                     /gene="lsrF"
                     /locus_tag="LAF92_002986"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBI9791955.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            21367..21657
                     /gene="lsrG"
                     /locus_tag="LAF92_002987"
     CDS             21367..21657
                     /gene="lsrG"
                     /locus_tag="LAF92_002987"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBI9791956.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            21714..22472
                     /gene="tam"
                     /locus_tag="LAF92_002988"
     CDS             21714..22472
                     /gene="tam"
                     /locus_tag="LAF92_002988"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBI9791957.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GIHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLAESEQQLFL
                     TRYHQMLEEQYPLQENEQILLAFPRLFIVARRTE"
     gene            complement(22476..>23112)
                     /locus_tag="LAF92_002989"
     CDS             complement(22476..>23112)
                     /locus_tag="LAF92_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791958.1"
                     /translation="RSLLREVKTTLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVL
                     AHYLKTEDLQRVLASNSPANRILLIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVL
                     AGCERIAYTPIPFAYTLILHRTVYLFCIMLPFALVVDLHYMTPFISVLISYTFISLDC
                     LAEELEDPFGTENNDLPLDAICNAIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         5772 a         5788 c         5397 g         6155 t
ORIGIN      
        1 gaaacttcag gaagcttgta ctcgacaata cagtttgagt ttttatcttt gccccatgaa
       61 acctgtaatt gccctgactg tggaagtcca gtcagataaa cctgaccatt ttccgcgaca
      121 atgctgccat ttttattctc tccgtgtgtg acaattgcac cgaatggaac gctcttatta
      181 ccgtacttca acgtcattaa tactttcccg ccgatttgtg cattaaatgt tgctctggca
      241 atagcaccgt gagttgggat gacagtgacc acggtttcat tcagttcaac attatctgca
      301 agggaattcg cgttaagagc aacacggttt tctctatatt ctgtcgcaaa tggtaatatg
      361 gcatagccac gccagtcggt atgaattccg gtctggttct ctattttgac attatcagca
      421 ccaggagcct taaccagaac cattgtgtcg cccagcggct gtccaaaggt gatgccatca
      481 gcatgagcaa taatcccacc actcattccg taataaatct ggctgctgtc accactccga
      541 ctgtaaccga cattagtatt accataagct ccacgataat taagagaact gtaaccactg
      601 gtgccagacg atgtattacc tccgtgggtg ttaccgacct gaacgctata attcaggtta
      661 ttatccggca gcagagtgcc ataaaccccc gatagattgg tcatgccgcc tttcaaatcg
      721 tttgacatac tgtaactggc gtttgaatta cgaaatgccg actgactgtc tgtacgcatc
      781 caatgactga agggaacatt aagcgtaaaa gcgagtaaat gatcccgatc gttttgccat
      841 atattattgg aatagctgta attcagcgaa gtcgtaatat caccaaacgg cacatttaat
      901 ccaaatgata tttgctggtc gctgcgtgac gtgttccagt aactttggcg actggcactg
      961 aaaaatgtcg taccgtaatt tccaagctgc tgagagatgc ttatttgttc ctgaccacgc
     1021 ttactgtaga acagattaaa ataatcaata aattgtgtct gctcattggt gtctccggta
     1081 ggaggcttga cggtgtaacc actcattcga ctgtaggcac tgtcggataa gttataaaaa
     1141 ccttgcgtag aatagcgata tcctgcgacc tggatattgg ttcctgtctg gtagaaggat
     1201 ttgctgtaga cggatttaac cgattgccca ctgtgacggg tgccatctgc aagtgtcgta
     1261 ttggcctgcg tgatatcgaa agaaaaggca ccaaaaagtc ctaaatcttt cccaatacca
     1321 aggttgaagg cctgataatc ttctgcaatt tgcattccgc cataaggtgt ccagtttcct
     1381 tccagaccat gcatcaagct accttgtatg aacttgggag agctttgcag gttatttcca
     1441 ctacgatatt cccccatggc aagcgcataa cgcgtatatc cagcacgttg gagaaccgga
     1501 acagacgaat aaggaacata taatgtctga atactgccgt ctgcctcttt tatggttact
     1561 tgcagatcac caccattggc cgcagagttg atatcatcaa tagtaaaagg gccgggtgga
     1621 acagtagtct gataaacatc gtatccgttc tgttttacac tcacttgtgc ggttccacgg
     1681 gcaataccat gaatcaccgg agcgaaacca tgttggctat cgggcaacat cgcttctgtt
     1741 gaatttattt tgaggccacg aaagttcaca ctatcaaaaa tatcgccatc ggtataacta
     1801 tcacctaccg ttaaccgtga gcgtaaggga ataatgtcac gttcagccga cgtattgata
     1861 tgctgccatt tattgctgtc agatgaattg ctactcccac tgtaataact ccacgttgag
     1921 ttatcgcgca gtcgccaact accaatgttg atgccactct gtaaattcaa atatgcatag
     1981 tttgattttc ctgcattatg gttactacga ttattaatag aattaccatt aaaactatag
     2041 tttagaagcc cagcatttat tccttcttcc cacagatcag gagaaacata gccgcgagcc
     2101 attctgccaa cataaatttg cggaacggag agtgacaaat gttgctgacc aacatcaaac
     2161 tcagttgatg cgtcggccaa acgatcctga agtggtgtgc atgcgctgtt gtccggcaca
     2221 tgtttttctg ccgaatgttc tttattgtct aataacgctg atttttttat accaagactg
     2281 acaagaagat ctgtactcag gcatgggatg agttctgcat tattatcatc tgcaataaaa
     2341 gtaatatccc gactagtcat aaattcatcg ttcaaataaa tgtcgacccg atatgtacca
     2401 ggtggtgcct cgcgtccctt agtaaaggcg gataaatcga cagattcagc aagatcattg
     2461 cttaaaaacc gtggattaaa ataatactct gcgtcaacgg gaaataccgc agttgctaag
     2521 ccaagggcaa tggtaagccg tgcaaagcga ggcattatta acacctgatg catacgatat
     2581 gccttaaatg cggcacgaat ggctgtcatt attaattccg attgagttta ttattaatta
     2641 ggtcactggc ttactgcatg gttgccttaa tacgcggagt taatgcaccg taatcattga
     2701 tcgtctgaaa actgatatcc ccggttgcag catgcgtgat atccaccgat acttcacctt
     2761 ttggcggcac catggtgttt gggagattgc tatttcctgc tttcatattt gttacggtaa
     2821 tgaaataggg cgttggatta ataagcgtga gtttgctacc gctgcgacga aaccgaagca
     2881 ttgcaggcgc ttcttcaggt gccattgcca ggttcgtggg gcgataaaac atttttatgc
     2941 ggctaataat tgccagctgt aatgtattct cattcttctg gtctttttcc atagcaggaa
     3001 tagctttaac attcacccag aacaggcttt cacggtcgcc tggtaactga tgattagtag
     3061 cgttgattat ccgcagcgta ttctcttttt taccttgcat ggaaaaaagc ggtggggtaa
     3121 tgacaaactg ggtgtctttt tggtcacctt cattttctat ccatgactga atgagataga
     3181 cactggcagg atcattattc gtgacaggca ataaaacctg tttctggttt gccggataaa
     3241 ttacccgcgt cgcacccagt gcgacagctg cgttggcaac ggtgctatgg caagcaaaga
     3301 gcaataaaag taacactgtc gccataaagg aaatagagta gggggtacgc gtagtttgca
     3361 tctgatgcat tctccatgtg ttaagaggat aagcgggaaa accgttacat cagacaatcg
     3421 attaatgttg ctaatacatt ggcgagtcgc cgggtttcca gacttaatgc gtttgtaata
     3481 aatgttggtt ctcttttaat aggaaaacct tgctacaaag taaatatttt aaataacagt
     3541 aagtttaact actgttgttt attgatagtt tagatattat gtgatttttc atagcgctcc
     3601 aaatacaaac catttctcat taaaaatgag atgaatattg gtaaattgct cgggtgtgcg
     3661 tagccatgcc acacacaccc tgtttcaatt tcagatgacg tatagccacc ttaagattga
     3721 attatagata ttgaactttg aaggtcgcat cagcgtttgc agtaccagct acggactgcc
     3781 cgagagcaat ataacgagcc tggaatggca gtatattcgt gccatcaatc aagtcggttt
     3841 tagcactaaa agtggcccca tcaagtatta atacctcacc cgtacggtcc aaaatttgaa
     3901 taccgacatt ttgggcagag cctgccgctg aactctgcag cgctaacgta tcgtcattac
     3961 tggtgacagt agtacccagg aatgcaacag ctgcattact ggaaacattg gtatcacaat
     4021 cattcagctt gatattgaag ccaacggcgg agctgaggtc acccgcttta gccaggcgag
     4081 aagaacgaac ctgacccagc tcaaccgttt ggttcattga ttctgaatca attgcacaag
     4141 cggcatttac cacttcacct ttaaaatgaa taacaccgcc attaacagtg gtcgttactg
     4201 attcatcacc ctcggctgcg cttaccgcag cagacgacat cgccaacaca gaaacgacaa
     4261 tcataccaac atgtttgagt ttcataattc tccaaaattt atatttaagt agctgaataa
     4321 agagagttaa caattaagtc attctgttat tgcttaaaaa caagaatcat tcttgatagg
     4381 ttgttgccct gagaatatag tttccagggt tattacattt cacgaagaac gataatatct
     4441 ttaaatcaca aggacaccta atcacaacaa gtttattgtg cacttgttga agagagtatt
     4501 tgcaaaaaca ttttatgtcg ctaaataaca tgacgatttc attatgcatg ttatttataa
     4561 accgtgacta aacagaatat tattgcgaac aagggaagtg tcaggaaagg tcaagggcta
     4621 ctaatggtta atatggctat aaatcattag ctatggttaa tgtttgcttg ctgaaactaa
     4681 caccgaattg ctctaaacag ataagtgcag ggtttattaa agctgaccga cagactttct
     4741 taacctggta atatctctct tgggtgactc tccaaacatc cgcgaatatt cccgactgaa
     4801 atgggatagc tttcgtaacc gacagcatag gctgctgtgg tgacatcgta atgttcattc
     4861 agcatcggtc gcctgacttc attgagacgt agccatttct gataccggag tggactcatt
     4921 cccgcttgat ccgtccttaa ataagtaaca gcaccctctg gtgttttcgt atgcagtgtc
     4981 tatgcgacag acactgcgct cttagcatgg ggcagggaat aaccaggcta tttctccatg
     5041 cacaggccaa ttatgaaaga ggagaggctt aagttcttga accctgaacg aataacgact
     5101 accagaaaag cacttcgcat cactcagaca tgatttaaca taatatacat tatgcgcacc
     5161 agtataaacc aaggaagaat ccagtcgttg gcggtcatga ttgtcgtgct cattaacaac
     5221 gaccaaaccc catatctcac ttactaccgt attctcggct taaccgtcca tgcaacctca
     5281 acacattggt ttcaactgcc gtcaccacgt tctccggaaa atctgtcggt aatgaagtct
     5341 tcacgttatc cagtgctgcc ggaatcattc tggcaaagtc acttaggatt tcatgcatct
     5401 gcacttccgg gaatctcagc acctttgctg tcgccaaata atgtcgcgga taaattttat
     5461 cgattgccgt ttttttgcct ttggatgcgt taagccccat tgccagtttg agatcgctga
     5521 tgtgtattcc cgtaccgcca aggaccggaa atgctgaaat gatgtcgtaa aatggcgtga
     5581 gtcgataact gccgccagcc tgaataaata cggagaagtt ttttgcatgg ccatccgttg
     5641 cgccaatcaa ccactggaag acctggaatt tcataaaatc ataacgatcg cgaagcgcct
     5701 cgctggaccc catcaaaaac gccatgatcc gcgcgatgcc tgggcctcca tctgattcat
     5761 atttcacaga tgaaggtaaa ccgaatgtct gacacatatc ctcctgtggc aagcgaagta
     5821 aaaccgttcg ctcagtattc caacgcctgt caaaacgttc gaccgctaac gcgcgcaccc
     5881 ttcccgcttt aatgatttct gcgtccggaa cattcaaccc aagttctttc gccagcagca
     5941 gacagtaata ctcattatca acgctttggc tgagatcgag cgtcgcattg ggctgcctga
     6001 tttcgccaat cggtaattta atgatgtgcg tcgtcggcgt tattcctttc ggaatgcacc
     6061 agtcattgcc tattctaagc agcgctgtct tctcctgcgc gccagcaacc gagatgcgaa
     6121 agtcattttc ttctctaatc atgcccagag ggatatctgc tttataagcc gttaatactt
     6181 cttcaagcct ggcttcagta agcttttccc atgccattat cgggcgcgtt atggtttcat
     6241 tttcgggtaa taacgttacg gcaccaacgc tgtctcgccc tatttctgac aataaatcaa
     6301 acggttgtct ggatttagca tgataacgtt taacgatccg gtcacgtaca atcgggctat
     6361 cgggtaacag gttatcgaag aagttaaata cggcatctga ggtgatattc cccctctgca
     6421 atggcagcga aagtgacaac ggtctggcat aacggctggc taaccactcc ggtgcatact
     6481 taaaggtgtg cgcgccgttg gctaacttcg ttaactcgcc tacccgctgg ttgttcatcc
     6541 aagtgacaag tttaggcatt accactccag atcttgctgt tctgttgatt ctggcgaggc
     6601 atttttcgtg tcgcatagtg tcattgagag ttcaagcgac tgtaaaatct taaaaaatgt
     6661 cgtgagcgtg gtattgtcag ggttgttttc gaaattggaa attgtcgcct gcttaatgcc
     6721 aatttttttc gccagctcgc tctgcgtcca gccattttgc tggcgaacca gtttcattgc
     6781 attcgccaat tgcgttgggc tatagatctt ctgaaagctc atcatgtcat acgtccacag
     6841 caaatttatc cgcttaaggg gatattatca gttttatcct ttagtgagga taaagtcaat
     6901 ctggaaacac atcttatccg ctgtacggga taaacgctgc gatatccgcg atcgcggata
     6961 aaagggaggc tttaaagcct ttgaagtcgg caatattaag ccgcatgcca tctcgacatg
     7021 cggcttatac ggtttaccag ctatatttca ctcccagcat gccctgagta tcgctatagc
     7081 ctttatcacc tagttgcaca ccgacattcc cccacaggct aaggttgtta tttactttcg
     7141 cctcaacccc ggtacgtact tcaccgagat tacgcgcacc ttcacggcct acggtttgac
     7201 cattcatctt cacggcgtag actttgctgt tgttgatcca attcgcttca atgtaaggct
     7261 ggaactcacg ctgtttacca tcgtcacgct ggtgatggct gttcaggtag gttttcaccc
     7321 caagtcgcgt ttgcacattt ccgtcgcctt ccgtttcaat gcgcgttccg tctttccggg
     7381 tatggtcgga atctttcaca cccatccagg tgatttgcgc ctgtggctgg acgtaccagg
     7441 tattcagcgt ctcttcgctg ccgctaaatg ttcccgcttc aaaggtatac ccaccctcaa
     7501 cagaggccgt cacaccgcga gaatcatagt cgtcagcaga acggttatcg gaactgacgc
     7561 tgttatcaaa ccagttatac agcgcccagc tgtcaacata agcgccggtc ttattcgcat
     7621 cgttctgata ccaggtcgcg tacagcccag cgctgtaacc gctgatgcgc ccatccgatt
     7681 tataacccac acgattactc tgagtattac tgtgctgatt agcgtaacct gccatcacgc
     7741 caagatgcca gcgatcctgc gcgttgctac tccactgcgc caaatcgccg cctagctgca
     7801 atacatagcg gttagcctga gtatttagct gaccgtcacc ggcccttgaa cgttcgtgcc
     7861 ctccgacatg acgcatccac atactgcttg ccgacccctg agaatgcagt gaatctatat
     7921 actgcggctc acccagacgg tcgtgtaaac gatggctaaa cagcgagttg gctgcggcaa
     7981 tgttgctgat atagcttccg gcctccgggc gataaactga tgggccttcc ggatccacaa
     8041 cagggggatt attgatgggg tcgggcgtat ccggtggcgt tacgccgtcc catttactgg
     8101 tcagatacca gtttttctcg tcattcccct tccctttagc cagcgtgtag acgtaagccc
     8161 cagcttcgac agttccggtg gtcagcgcga agttacctgc agaattaccg ccaacctcaa
     8221 tgagttcaat accgttgacc gtttgtgcac cgacgccgcc aatgttatca acccgaacgc
     8281 gagtgttccc ttgggtatcg cctttcacgt tcattttatc ggtgggcgaa ttatcgccgc
     8341 ccagcgtcgc gttgaacgtc atcaggccat tgtttccggt atagttgccg ttaacggtca
     8401 gtacattccc cggtttcccc ccttcgctat tcatttgaac cgtgccgcca ttctccaggt
     8461 tgccgccgat tgtggtttta gcgacacgca gtgtcccccc aggcataacg ttcacatcac
     8521 ctgcagtggt gacatttccc gacatcacac cgctgttatg gatattaatg tgttgacttg
     8581 ccatattaat ggcagagtcg gaaccgaaag caatttcccc ccccacgata tccgtcgtac
     8641 cctgatacgc cgcggaatcg ttgttaagag tcaggatccc ggtacccagt tttgtcacat
     8701 caccgctacc gctgatactg tgcgtcatcg cccagctacg ctcagtggag atggtgagca
     8761 ggccattatt gataacggaa gcatcaccca ggtttttctg ctcgttgacg ctgagcgcgc
     8821 tgcctgtatc aatgttgaat tgccccacaa agcggctatt atcgccatcc agctctacat
     8881 ctgtcctggc ggttgcgctc acgataccct tcccgcttat gctattacgc agttcgccag
     8941 tcacgttttt tagcgttaac agaccgtcat tggcaatatt tcctctgcct aacccctgag
     9001 tattatcgag gctgacttcc gcatttgggc taatgctggt taacgcattg tagcgtgcat
     9061 taagcccctc gatagccagc gttccccctg taacattcag gtttccgcca cctgtcagtt
     9121 caccctgact gatcccacct ttatttaccg tcagcgcccc ctctttgaac aaaacagtcg
     9181 aacccatctg cccggtgaat gtctctaccg tctgcgtcga tccattaaga tcgacgatgg
     9241 ccgcgttgct gatgttcagt tcccgggtgt tgccaagtgc accatccgca tcggtacgta
     9301 atgtccccat ctgaacgtag gttgccccct gatagtcgtt ctgaccgttg gaaagcgaaa
     9361 cctgtcgcac cgtattgatt gccagatccc cttcaccacc gatttttgcc gacatatcgg
     9421 ccgttgcgcc ataggctccg ccatgttcgg ctaacgtcag cttttgccca ccatggatgt
     9481 tcagcgcttt cagcccatag ttaacgtaca aaccatcacc tggtgcggtc gtgaggcgga
     9541 atccataatc accctcggcg accaccgttc cgttttgcgc aatgtgtaac gttttgtccg
     9601 cttccacctc gtcgccctgt aaatcacgta acgttaatga gccacccgac ccaataaccg
     9661 tttgcgcctt caccagttga acgccgacat ggctatcgtc gtgttccagc aaattgagcg
     9721 tcgtcagagg gttattcgcc atgggatcat tccacggttt aggcacgtcg ataagcacgt
     9781 cgcccgtccc gtttacctga tagttacggc ctttccaggt gtagtcaccc gcgccgacaa
     9841 ccagcgtatc gacgctgata tatccctctg caagcgtcgc agcaggaata tccgtatcga
     9901 aaatgagcgt accgccattc atggccagtc caccaatgga ttgctctccc acttttaccg
     9961 atgtggtatt ttcactgtca gactgcaaca tcgcgtgagt aagcgcagcg gtgttgtcgc
    10021 gttccagagt gaaggtactg tctttcagtt gggcaacacc ggcgaattca gtccctgttg
    10081 catgggtaaa gccaaacatc ttgtcgtagg atgacagctg cactcgcatc aagccatcgc
    10141 ctttgagcgc attggtcagc gtgtaatctc ctgcactgtt cgttgatgcg agaatgtcga
    10201 gcgtaccggg gcttgcaatg gtgacttcac tcgaacccat gcccgttacc ccatctgccg
    10261 tatgagacgc aatggtcagg aggccgtcat tgaccagcgt tttaccgcta taagtattcc
    10321 ctgcattgaa caccgtatgg cctgcatcat ggatcacttt tccgtcttta tcgtcacctg
    10381 taatcagcat atcgacctgg tagccggcat cactgttatt agtatgatta aagacaaaaa
    10441 caccttcgcc agaaccaaac tctactttcg tcgcgttggt tatatacccg gcatctgccg
    10501 ccgcctcacc gtgagctgca ccaatgttta gcgttcctac agcaggttca aaaacaccta
    10561 agtaaacttc tccaccttcg acattcagcg tgccgctatt cgtcagggtt aacgtacccg
    10621 taccagatgt ccctacgtac atattgaatg tttcaagaag agagttctgc ccatccaccc
    10681 tcacgtcgcc cttacttttg tcattgagag ctatctgtgt atcacgcgct ttaactatac
    10741 ctccggtggt aatattcagt tcacccttgc ctaatacacc gacctgtagc aattgtgcgt
    10801 tagtagatga tgtctttaaa ttccagagac tgtccgttga aatattaact atccccttac
    10861 cggaggcatt tcctccgata aatccatagc ccgttgaatt aagggtggca ccattactga
    10921 catttacgat accgctacca aaattaccga cattgatacc cacagtatcg aacgatgatg
    10981 tcgcatccat caggttcagt gtgccgatac cgcttgcttt atcgcccaaa aaagtaatat
    11041 tttgcgatac gaacttaccg ccatcgctaa tattgaggtt tccgacgccc gcaacaccaa
    11101 tggtggtata gacattctta ttgacctccc acatcccccc cgtggtgatg ctgacattcc
    11161 cgacgccggt ttctccataa ccgagtgagc ttcctccgtt gctgacaaca agcccttcat
    11221 tactgatatt catttcgccg tggccgtcat aaccaagatt agttccgaga ttagtgataa
    11281 cggagttctt atccttaaca gtaatgttgc ctgtgcctgt ttctttcatc cccgcagtga
    11341 taattcccga atctacttta ccttcacgcg agacattaag ttcaccgtcg ccagcatcac
    11401 cgatatagat atagcggaaa gcttcgcccg ttccgaggaa attccaatgc cctttatcgg
    11461 tcacgttgac gacaccgtgg gaaccgtcct gaacgcccac taatgaatat tctttgttat
    11521 taattagtcc attattggaa atattgagtg tgccattacc gaaatatcca ttatagagtc
    11581 tgcgtaccgt gatgacagag tcttgatcct gcacattcag ggttccgata ccggtagcat
    11641 tgccaccgat aatcgtgttt tcagccgtaa tcagtccacc ctcgcgaata gtcgcttccc
    11701 cagttccttg attaccaatt tgaaattcaa ttgaggaatc gttatttttt attagccact
    11761 caccgccctt ttcaacgaca acctggccat tactgcccgc ctgatagcct aaaatggcga
    11821 ctattgaact cgtgacgtaa cccttatccg taatatttaa tgagcccgta ccgtaactcc
    11881 ctatttcgaa taattcggtc gtcagaacag agtcctctcc ctcaacattg accgtcccga
    11941 cgcctcctgt cgaggaacct agtcttaaat agcctccatc aacgtgacct ttctgtttaa
    12001 tattcagcgt ccccgttccg gattgaccca catttaaagg ccttgcatta tttccgctat
    12061 catacaatcg ccaggtgcca cccaaaacat taacggtgcc ctctgagtcc tcattagcgc
    12121 caatgacgct ggtagtaata gtggttaagg aggcattacc gccagccaaa atattaagca
    12181 ctccagtgcc ataccagcca accaggtagg catcataagc aacatcagta tcaatattgg
    12241 taatctgatc attatcaact tccagacttg caccagacgc tgaaccgctt agtgccagca
    12301 aaacacccag cgtcaatcta ctgaatttcg ttgtcagtcc agaggattta cgcaaattaa
    12361 ccgtcgatgt tttacctgcc ctgcgagtta attccgagca ggcctgaaat acctgtagag
    12421 tgcaattcca tatcacgcga tagattctat tcatataaac gctccatata caaacaatac
    12481 agccacgaaa gagatgagtc gtcagacatt aaaaatgaaa cttattaaat tgtcagaggt
    12541 ctgtattgag tgttagttgg aggtgggaag gtgtcaattt ggctaatttg ctaatgcttt
    12601 cgaattgata gattaaaaat tgcatagttc ttattttatt taaacatgaa ctatgagtta
    12661 ttgttgctta ataagaaaac tgtttctttc aataggaaaa ttatcatcat catatttaac
    12721 aaagaatagc actaattgct aaaaatcgaa gtttattaaa ccccttttgc atccattagt
    12781 gtctttagga atattcgcta taaaataagg gttgtgctta atgctttaag aaaaatagca
    12841 attttccctt gaatatagta ctggtgatgg aacgatgaat ctgcaagctg gcttttaaca
    12901 agccagctct aaaagaaggg aaataagaat aactatactc aaaaactaac agccacggtc
    12961 atcatgatgt ggctgtcaat gaaactataa cccaggcgct ttccataacg acgtcgtcag
    13021 tccatgatca accagcccca gctgatcctg ataaactgcc tgccacttat cgcgtgaatc
    13081 ctgataaagt tcatgctttt ccgggtctgg tgtgtgcgtc cgttcccagc gaaccaggcg
    13141 ttctccggtt tctgccattg atgaaaaaat tccggcaccg acgccagctg caatggcaca
    13201 tcctaatgca gtggcttctt tgaccaccgg aatattgacg ggtaatcccg agacatcagc
    13261 gagaatttga ctccataatt tcccttttga acctccgcct gcaaagacta acgatgaagg
    13321 atgaatattc gagaaatcag caatttgctg caagttacac gctgatacaa tcgccgcatt
    13381 ttcttccagc gcacggaaca atgtcgcttt gttacattta tccgggtcaa tggacaagtt
    13441 aataaaggaa ggcgcagcgt gataccaggt tttaaagcgc attctgtcgg agaagatcgg
    13501 cattacgccc cacgacccag gcggcacccg actggccatc tcttccagca gcgtataggt
    13561 gtcgatgcct aaacgttcgg caatcagttt ttcttcggca cagaaagcat cgcggaacca
    13621 gcgcatggtg agtccggtaa aaaagcttat agattcagct tgtaccatgc caggaataac
    13681 atgaggatta acgcgcacgt tcatttctgg gtctgtcacc ggcgcggcta aatttacaac
    13741 ttgctgccag aatgtgccgc caagaaccgc ggtttgtgcc ggacgcacaa cgcctaaccc
    13801 aaggcaacca agctgcacgt cgcctcctcc aacgaccacc ggagtgcccg ccttcagacc
    13861 gcagagttcc gccgcttgtg aacttaccac gcccagcaat gtgccggttt ctttgacagg
    13921 agaaagaata tcggcacgca ggccagccat atccagcaat gcaggtttcc agtcacgggt
    13981 ggttagatca agaagtcccg tggtgccagc gttagaggga tccaccgcca gttcgccgct
    14041 gagcatatag gccagccagt cgctgatcat ggtgatggtt gatgcctgac ggtaaatatc
    14101 ggaacgatgg tgcgccagcc aaagtaatct ggggatggca cttaaagcca gtgtttgtcc
    14161 ggtcgcgcga taaacttcgt tttcaaaggt attgttgtgc agttctttaa gttcgctaac
    14221 ttcgcgtgcc gctctggcat ccacattggc gcaggcccag atcggggctc cttcattatt
    14281 atataaaaca atgccttcac gcatcgaaca tgccgaaacg gcagcgatat actccggggc
    14341 tatgccggcg ttgtgcagcg cctggcgcat acactcacac gccagttgcc agtttttgtt
    14401 gagatcaaat tccatagaac caggaacgtc cggtactgcc aggtgccgcc actccgcctg
    14461 tcccactgct atttgattgc cttccaggtc gaatatcaca gcccgaatac ttccggtacc
    14521 tgcatccagc gccatcaggt aatactttga ttctgaaggg gtaaagagtc gagccattag
    14581 attatcctcg gcttatgcgt tctgtctgtt cctctatacg ttctccatca ttcccggtaa
    14641 taaggtcatg caaatttaac tacgtaaaat cgccgctgct gtgtcctgat cggtaaccag
    14701 tgcgttgata taaccgcctt tcattgcagc ggcaattgct tcggctttat tttctccccc
    14761 tgccacgcca acccggacgg gtatggtctt cagcgagctt aaaggtaagc caatcagttc
    14821 gttatgtatt ttaatatccg tgacaacgtc accttttgca tcaaaaaagt agcctaaaat
    14881 gtcgccaacc gccccttttc gaccaatcat taactgttcg ccctggctga tataaccgga
    14941 gcgaatgatc gtcgcgtcgt cctgttgact gacggcccca atgccgacaa tcgccacatc
    15001 ggctgcttgc gcggctaaca gaacatcttt gacgcaattt tcatttttta gcgtacgggc
    15061 aatgtcagcg gaggatgccc gcaacggagc cggaataata ttcacactgc acgccgcgtt
    15121 aagctgcccg atacccgtca tataagaacc gacgccaccg gagagcgtga ccaggcgaat
    15181 ttgctgtgac gaaataaaac cacttaagcg ttgcagcgta ttcatggttg cctcgccaaa
    15241 accaatcgcc agcatctgtt gtggttgaag taaactcatc aacatatgcg ccgcgcctat
    15301 ccccagtcgc ccgccgacat cagcatccgc aagcccaggg atcacccgga catgttgcag
    15361 cgaaaactga cgacgtaatt gagtttcata ttccagacag ccttcaaagc gagaattaat
    15421 ctgtacgcga ataatgccgg actgatgccc tttctccagc aagcgcgaca ctttcaaacg
    15481 ggtcaggccg agacgatcgc tgatctcgct ctgggtcagc ccgtcgtgat agtaaaacca
    15541 cgcgatccgc gcgacctgtt cttcttcaca cattccctgt tctgaaattg ccgaatcgtt
    15601 gattgtcata attcattctt cactttgaac atatttaaat ctttaatgca attgttcagt
    15661 tcttgcgcat ttatatctgt gatggcagcc acagtttgac tctacgagca tgaacaaacg
    15721 caaccgtgaa aatcaaaata gcataaattg tgatctattc gtcggaaata tgtgcaatgt
    15781 ccacctaagg ttatgaacaa attaaaagca gaaatacatt tgttcaaaac tcacctgcaa
    15841 aactgaacgg gggaaatatg caaacgagtg atacccgcgc gttaccgcta ctttgcgccc
    15901 gctcggttta taaacagtat tcaggggtca atgtcctgaa aggcatcgat tttacgttgc
    15961 atcaggggga ggtccacgcc ctgctcggcg gcaatggtgc cggtaaatcg acgttaatga
    16021 agattattgc cggtattacc cctgctgata gcggtacgct ggagattggg ggcaacaact
    16081 acgccagatt aacgccagtt catgctcatc agctgggcat ttatctcgtt ccccaggaac
    16141 cgctgctttt cccaagcctg tcgataaaag aaaacatcct gtttgggatg gcaaaaaaac
    16201 agctctccat gcaaaaaatg aagaacttgc tggcggcgct gggctgccag tttgatctgc
    16261 atagtctggc aggatcgctg gatgtcgccg atcgccaaat ggtggaaatc ctccgcgggc
    16321 taatgcgcga ctcgcggatt ctgatcctcg atgaacctac cgcctcgctt acccctgcgg
    16381 aaaccgaacg cttgtttact cgcttgcaag agctgcttgc tactggcgtg ggtattgttt
    16441 ttatctcgca taagctgccg gaaattcgcc agattgccga tcgaattagc gtgatgcgcg
    16501 acggaaccat cgccttaagc ggcaaaacca gcgaactgtc taccgacgac attattcagg
    16561 ccatcacgcc agtggtacgg gaaaaatcgc tctctgccag ccaaaaatta tggctggagt
    16621 tacctggtaa ccgcccacaa catgctgccg gaacgccggt gctgacactg gaaaatctga
    16681 ccggcgaagg tttcaggaat gtcagcatga cgctcaatgc cggagaaatt ctgggcctgg
    16741 ctgggctggt gggggccgga cgcacagaac tggccgagac gctctatggt ctgcgtactt
    16801 tgcgtggcgg acgcattatg ctgaatggta aagagatcaa taaattatcc actggagaac
    16861 gtttactgcg cggtctggtt tatctgccgg aagatcgcca gtcatccgga ctgaatctcg
    16921 atgcttcgct tgcctggaac gtctgcgccc ttactcataa ccttcgtgga ttctgggcga
    16981 aaaccgcgaa agataatgcc accctggaac gttatcgtcg ggcgctgaat attaaattta
    17041 accaaccgga acaagctgca cggacattat ccggtggcaa ccagcaaaaa atcctcattg
    17101 ccaaatgcct ggaagcttcg ccgcaagcat tgattgtcga tgagccgacg cgcggcgtgg
    17161 atgtctcggc ccgtaatgat atctaccagc tgttgcgcag catcgccgca caaaatgtgg
    17221 cagtgctgct tatctcctcc gatctggaag agatcgaact gatggcagat cgcgtgtatg
    17281 tgatgcatca gggcgaaatt gcccactctg cactgaccgg gcgcgatatt aatgtcgaga
    17341 ctattatgcg cgttgccttc ggcgatagtc agcgtcagga ggtgtcatgc tgaagtttat
    17401 tcagaacaac cgtgaaatca cggcactgct ggcggtggtg ctgctgtttg cattacccgg
    17461 ttttctcgac cgccagtatt taagtgtgca aacgctgacc atggtttata gcagcgcgca
    17521 aatcctgatc ctgctggcaa tgggcgcgac gctggtaatg cttacgcgca atattgatgt
    17581 ttcagtgggt tcgattaccg gaatgtgcgc agtgctgttg gggatgttac tgaacgcggg
    17641 gtattcacta cctgttgctt gtgtcgcgac tttactgctt ggtttgctcg cgggattttt
    17701 caacggtgtc ctggtcgcgt ggctaaagat ccctgccatt gttgccaccc ttggcacgtt
    17761 agggttgtac agaggcatca tgttgctgtg gactggcggt aaatggattg aagggttacc
    17821 cgccgaactg aaacagctct ccgcaccgct gctgtttggc gtttccgcga ttggttggtt
    17881 gacgataatt ctggtggcat ttatggcctg gctgctggca aagacggcgt ttggacgcag
    17941 tttttatgcc acgggcgata atttacaggg cgctcgtcaa ctgggtgttc gtacggaagc
    18001 cattcgcatt gtggcatttt cgttgaacgg ctgcatggcg gcactggcgg gaattgtgtt
    18061 tgcttcgcag attggtttta tccccaacca gaccggtacc gggctggaga tgaaagcaat
    18121 tgcagcctgc gtgctgggcg gcattagttt gctcggcggt tccggtgcga tcatcggtgc
    18181 ggtactcggc gcatggttcc tgacgcagat cgatagcgta ctggtgctgt tgcgcattcc
    18241 ggcatggtgg aacgatttta tcgcgggcct cgtgctgctg gcggtgctgg tgtttgatgg
    18301 acgcctgcgt tgtgcgctgg aacgtaatct acggcggcaa aaatatgccc gctttatgac
    18361 gccaccgcca tccgttaaac ccgcttcgtc aggtaaaaaa cgggaggccg cataatgcgt
    18421 attcgctacg gttgggaact ggctcttgcc gcactgctcg ttattgagat tgtcgcattt
    18481 ggtacaatta acccgcgaat gttagatctc aatatgttgc tgttcagcac cagtgacttt
    18541 atctgcattg gcattgtcgc cctaccgctg acgatggtga ttgtcagtgg cgggatcgat
    18601 atttcgtttg gttcgaccat cggcctctgc gccattgcat tgggcgtact gtttcaaagt
    18661 ggtgtgccga tgccgctggc gatactcctg accttactgc tcggcgcatt gtgcgggctg
    18721 atcaacgccg gattaattat ctataccaaa gttaacccgc tggtgattac gcttggcacg
    18781 ctgtatctgt ttgccggaag cgctctgctg ctttccggta tggccggagc gacggggtac
    18841 gaaggtattg gtggattccc gatggcgttt acagatttcg ctaacctgga tgtgctggga
    18901 ctccccgttc cgctgattat cttcctgata tgtctcctcg ttttctggct ctggctgcat
    18961 aaaacccatg ccggacgtaa tgtgtttttg attgggcaaa gcccgcgcgt ggcgctttat
    19021 agcgcgattc cagttaaccg caccttatgt gcgctctatg ccatgacggg gctggcgtct
    19081 gcggtcgccg ctgtgctgct ggtatcgtat tttggttcag cacgttccga tctcggtgcg
    19141 tcgtttctga tgcccgccat caccgccgtg gtgcttggcg gtgccaatat ttatggtggt
    19201 tccggttcca ttatcggcac cgccattgcg gttttattag tgggatattt gcaacaaggt
    19261 ttgcaaatgg caggagtgcc aaatcaggtg tccagcgccc tttccggtgc gctacttatc
    19321 gtcgttgtcg taggtcgttc cgttagcctg catcgccagc aaattaaaga gtggctggcg
    19381 cgtcgggcca ataacccatt gccataaagg atatcttcat gacacttcat cgctttaaga
    19441 aaatcgcctt acttagcgct cttggcattg ccgcaatctc tatgaatgtg caggccgcag
    19501 agcgtattgc atttattccc aaactggttg gcgtgggatt ttttaccagc ggtggcaacg
    19561 gcgcacaaca agcgggtaaa gagctgagcg ttgatgtgac ctacgacggg ccgacagaac
    19621 ccagtgtttc tggtcaggta cagttgatta ataacttcgt caatcaaggt tataacgcca
    19681 ttatcgtttc tgcggtttcg cctgatggct tgtgtccggc actgaaacgc gccatgcaac
    19741 gtggtgtgag agtgctgacc tgggactctg atactaaacc ggagtgccgc tcttactaca
    19801 ttaatcaggg aacgcccgcc cagttgggag gtatgttggt ggatatggcg gcgcgtcagg
    19861 tgaataaaga caaagccaaa gtcgcgtttt tctactcaag ccccaccgtt acggaccaaa
    19921 accagtgggt gaaagaagcg aaagcgaaaa tcgccaaaga gcatcctggc tgggaaattg
    19981 tcactacgca gtttggctat aacgatgcca ctaaatcatt acaaaccgca gaaggaatat
    20041 taaaagcgta tagcgatctc gacgccatta tcgcccccga cgccaacgcc ctgcccgctg
    20101 ccgcacaagc cgcagaaaac ctgaaaaatg acaaagttgc gattgtcgga ttcagtacgc
    20161 caaacgtgat gcgtccatat gtggaacgcg gcacggtgaa agaatttggc ctgtgggatg
    20221 tggttcagca aggcaaaatt tcagtgtatg tcgcggatgc attattgaaa aaaggatcaa
    20281 tgaaaacggg cgacaagctg gatatccagg gcgtaggtca ggttgaagtc tcgccaaaca
    20341 gcgttcaggg ctatgactac gaagcggatg gtaatggcat cgtactgtta ccggagcgcg
    20401 tgatattcaa caaagagaat atcggcaaat acgatttctg atgtgcatta cttaaccgga
    20461 gtaagttatg gcagatttag acgatattaa agatggtaaa gattttcgta ccgatcaacc
    20521 gcaacaaaat atccctttta ccctgaaagg ttgcggtgcg ctggattggg gaatgcagtc
    20581 acgcttatcg cggatattta atccgaaaac gggtaatacc gtgatgctgg cctttgatca
    20641 tggttatttt caggggccga ctaccggact tgaacgcatt gatataaata tcgccccgct
    20701 gtttgaacat gccgatgtat taatgtgtac gcgcggcatt ttgcgcagcg tagttccccc
    20761 agcgaccaat aagccggtgg tactgcgggc gtcaggtgcg aactctattc tggcggaatt
    20821 aagtaatgaa gccgtggcgt tatcgatgga tgacgccgtg cgcctgaaca gttgcgcggt
    20881 ggcggcgcag gtttatatcg gcagcgaata tgaacatcag tcgatcaaaa atattattca
    20941 gctggtcgat gccggaatga aagtgggaat gccgaccatg gccgtgactg gcgtgggcaa
    21001 agatatggtg cgcgatcagc gttatttctc gctcgcgact cgaatcgccg ctgaaatggg
    21061 ggcgcaaatt atcaaaacct attatgtcga aaaaggtttt gaacggattg ttgccggatg
    21121 tccggtaccc attgttattg ctggcggtaa aaaattaccg gagcgcgagg cgctggaaat
    21181 gtgctggcag gctatcgatc agggcgcttc tggtgtggat atggggcgta atattttcca
    21241 gtctgaccat ccggtggcga tgatgaaagc cgtacaggcg gtggttcacc ataacgaaac
    21301 ggctgatcgg gcatatgaac tctatctgag tgaaaaacag taactgcgga tttaaggaga
    21361 agaattatgc acgtcacact ggttgaaatt aacgttcatg aagacaaggt tgacgagttt
    21421 atcgaagttt ttcgccagaa ccacctgggc tctgtacagg aagaaggtaa tttgcgcttc
    21481 gatgtcttac aggacccgga agtgaattcg cgcttttata tttacgaagc ctataaagat
    21541 gaagacgcag tggcattcca taaaaccacg cctcactaca aaacctgtgt cgcgaaactg
    21601 gaatctttaa tgaccgggcc gcgtaaaaaa cgtctgttca atgggttgat gccgtgaggc
    21661 gaattcatca gttttatcta caattggggt aacgcgctga cgggagtaaa taaatgtctg
    21721 actggaaccc ctctttatat ctgcactttg ccgctgaacg atcgcgtccg gcggtggagc
    21781 tgcttgccag agtaccgctg gagaatatcg aatacgttgc cgatcttggc tgtggcccag
    21841 gtaacagcac cgcccttcta aaccaacgtt ggcctgctgc caggataaca ggcatcgact
    21901 cgtctccggc gatgattgct gaagcgcgca gtgctttgcc agactgccag ttcgtggaag
    21961 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
    22021 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
    22081 agggcgtgct ggcagtacag atgccagata actggctgga gccgactcat gtgctcatgc
    22141 gcgaagttgc ctgggaacaa aactacccgg atcgcgggcg tgagtcgttg gctggcattc
    22201 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
    22261 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
    22321 gtccgtggtt acaggatctg gccgagagcg aacagcagct ttttcttacg cgctaccatc
    22381 agatgctgga agagcagtat ccactgcaag agaacgaaca gatactgctg gcatttccgc
    22441 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
    22501 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
    22561 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg atcttccagt
    22621 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
    22681 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
    22741 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tataggcaat gcgctcgcat
    22801 cctgccagga ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct
    22861 gaaagctgcc cattgcggcg ctgaaccgcc aaccactccc ccattattaa caagatacgg
    22921 ttagctggag agttcgaagc cagtacacgc tgaagatctt cagtcttgag ataatgagcc
    22981 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
    23041 tgcagccggg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctcgcgc
    23101 agtaacgacc gt
//
LOCUS       DAEVZA010000077        22935 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEVZA010000077 DAEVZA010000000
VERSION     DAEVZA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.077"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(50..346)
                     /locus_tag="LAF92_002990"
     CDS             complement(50..346)
                     /locus_tag="LAF92_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBI9791959.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(560..1846)
                     /gene="thrC"
                     /locus_tag="LAF92_002991"
     CDS             complement(560..1846)
                     /gene="thrC"
                     /locus_tag="LAF92_002991"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBI9791960.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1847..2779)
                     /gene="thrB"
                     /locus_tag="LAF92_002992"
     CDS             complement(1847..2779)
                     /gene="thrB"
                     /locus_tag="LAF92_002992"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBI9791961.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2781..5243)
                     /gene="thrA"
                     /locus_tag="LAF92_002993"
     CDS             complement(2781..5243)
                     /gene="thrA"
                     /locus_tag="LAF92_002993"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBI9791962.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5269..5388)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5324..5389)
                     /gene="thrL"
                     /locus_tag="LAF92_002994"
     CDS             complement(5324..5389)
                     /gene="thrL"
                     /locus_tag="LAF92_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBI9791963.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5603..6289)
                     /locus_tag="LAF92_002995"
     CDS             complement(5603..6289)
                     /locus_tag="LAF92_002995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBI9791964.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6689..6829)
                     /locus_tag="LAF92_002996"
     CDS             complement(6689..6829)
                     /locus_tag="LAF92_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9791965.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6925..7641
                     /gene="arcA"
                     /locus_tag="LAF92_002997"
     CDS             6925..7641
                     /gene="arcA"
                     /locus_tag="LAF92_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBI9791966.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7701..9053)
                     /gene="creD"
                     /locus_tag="LAF92_002998"
     CDS             complement(7701..9053)
                     /gene="creD"
                     /locus_tag="LAF92_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBI9791967.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9111..10535)
                     /gene="creC"
                     /locus_tag="LAF92_002999"
     CDS             complement(9111..10535)
                     /gene="creC"
                     /locus_tag="LAF92_002999"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBI9791968.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10535..11224)
                     /gene="creB"
                     /locus_tag="LAF92_003000"
     CDS             complement(10535..11224)
                     /gene="creB"
                     /locus_tag="LAF92_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBI9791969.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11237..11710)
                     /gene="creA"
                     /locus_tag="LAF92_003001"
     CDS             complement(11237..11710)
                     /gene="creA"
                     /locus_tag="LAF92_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBI9791970.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11921..12790
                     /gene="robA"
                     /locus_tag="LAF92_003002"
     CDS             11921..12790
                     /gene="robA"
                     /locus_tag="LAF92_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBI9791971.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12787..13434)
                     /gene="gpmB"
                     /locus_tag="LAF92_003003"
     CDS             complement(12787..13434)
                     /gene="gpmB"
                     /locus_tag="LAF92_003003"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBI9791972.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDTPALDELQR"
     gene            13486..14007
                     /gene="yjjX"
                     /locus_tag="LAF92_003004"
     CDS             13486..14007
                     /gene="yjjX"
                     /locus_tag="LAF92_003004"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HBI9791973.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14092..14418)
                     /gene="trpR"
                     /locus_tag="LAF92_003005"
     CDS             complement(14092..14418)
                     /gene="trpR"
                     /locus_tag="LAF92_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBI9791974.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14508..16445)
                     /gene="sltY"
                     /locus_tag="LAF92_003006"
     CDS             complement(14508..16445)
                     /gene="sltY"
                     /locus_tag="LAF92_003006"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBI9791975.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFVRTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDKQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16656..18323
                     /gene="ettA"
                     /locus_tag="LAF92_003007"
     CDS             16656..18323
                     /gene="ettA"
                     /locus_tag="LAF92_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBI9791976.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18630..19862)
                     /gene="nadR"
                     /locus_tag="LAF92_003008"
     CDS             complement(18630..19862)
                     /gene="nadR"
                     /locus_tag="LAF92_003008"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBI9791977.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19883..21265)
                     /gene="radA"
                     /locus_tag="LAF92_003009"
     CDS             complement(19883..21265)
                     /gene="radA"
                     /locus_tag="LAF92_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBI9791978.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21314..22282)
                     /gene="serB"
                     /locus_tag="LAF92_003010"
     CDS             complement(21314..22282)
                     /gene="serB"
                     /locus_tag="LAF92_003010"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBI9791979.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22388..>22935
                     /locus_tag="LAF92_003011"
     CDS             22388..>22935
                     /locus_tag="LAF92_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBI9791980.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTL"
BASE COUNT         5458 a         6375 c         5671 g         5431 t
ORIGIN      
        1 agcgtcgcat caggcgctga atgccggatt gttacccggc atttgtcatt tagcggtgat
       61 gtttgcctgg accatgaccg ccgtgatgat catgaggagc tttcttatgg tggcgcggcg
      121 gtggcggcgg tccgtgtggg tgccagcgat tgcctcgcca ttcataatgt tgtttccacc
      181 agccgtggtc gcgccagtga cctccatccc agtaatagcc acgattatca cgatcgccta
      241 tctgtaattt tactgacggg actaacgtaa tttccgcagc ctgtgctgcc atgggagcga
      301 ccagaaccag ggaaagtgcg agtacgatag attgcatctt tttcacaggt tactcctttt
      361 atcattgccc gttatgggtc gatatccgga gattacgcga tgagaacgac ggttgttatt
      421 ctccgcaatc ctaatctcta agttaccgca tttattgaga atttctcctt tttccctgtc
      481 gtttttctcc ataatttctt cataaaaaag ccgggctgca taaaagcaaa cccggcctga
      541 ttgagataat gaatagattt tactgatgat tcatcatcaa tttacgcaac gcagcaaaat
      601 cggcgggcag attatgtgaa agcaagggta aatcagcacg ttctgccagc tcttttggca
      661 gatccaacgt ttcaccgaga atcgcttcca cgctctcttt aaatttcgcc ggatgcgcgg
      721 tgccgaggaa caagccatat tcgcctggat tcaactggtc acgcagcgca cgatacgcta
      781 cggcagcgtg cggctccgag gtgtagccca gttcttttaa ctcacgcatt gtctgttgcg
      841 tggtttcatc atccacggct gcataaccca gctctttcag ttgccagatt ttgcggcgga
      901 acaactcttc cacacgcggc cagttgttcg gctgactcac gtccatcgcg ttggataacg
      961 tcgcctgagt cgctttgggt gaccactgac cgtcgtgcag gaaacgtggc acggtatcgt
     1021 tcacgttggt cgcagcaata aaacgtttca ccggcagacc gagtgacttc gccagcagac
     1081 ccgccgtcaa atcgccgaag tttccgcttg gcaccgagac aaccagctgg ttgcgcgctt
     1141 cctgcggcag ctgcgcaaca gcttcaaagt agtagcaaat ctgcgccagc aaacggctga
     1201 tgttaatcga gttagctgag tttaacccca gcgccacttt cagttcttca tcatcaaacg
     1261 cctgcttcac cagcgcctga caggcatcga aatcgccgtc gatggcaaca gtttcgatat
     1321 tgccgcccaa cgtacagaac agtttttctt gcagtggact gattttgcct cgtggataga
     1381 ggataaccac tttcacattc ggtaaaccgt agaaagcatg agccactgcc gctccggtat
     1441 caccggaggt cgcggtcaga atggtcactg gcttatcgcc cgcaatatgg gtcagcattt
     1501 gtgccataaa gcgaccgccg aaatctttaa atgccagcgt tggcccgtgg aacaattcca
     1561 gacaaccgac atcgctttca acattggcga ccggagccgg gaaggcaaac gccgcgcgca
     1621 cgcgctcttc caggatttcc tgcgggattt catcaccaat aaacgccgag aggatcttcg
     1681 cactgcgggt gacaaaatcc agcttcagca tctcatcaat ttcagtcagg ctgaattccg
     1741 gcaggtcatg cggaaaaaac agcccctgat ttttgcccaa cccctgggtt acggcttgcg
     1801 caaagctgac ctgctcgttg tgatctttca gattgtagag tttcatttag ttttccagta
     1861 ctcgtgcgcc cgccgtatcc agccggcaaa tatgaacaaa accttcctga ttttgcaggt
     1921 agttcttacc caaccagtcg gcaacgcgct gggcggtatc cggcttgtca catagagcga
     1981 acaaggtcgg gccggagccg gagataccgc tcgctaccgc gccgatttcc gcgaccgcct
     2041 gccgcgcctg ccggaagcca ggcagtaacc gttcacggta gggttcagcg ataacatctt
     2101 tcatcagctt cgcggcaagc tcaggctgac gggaatagca ggcgtgaatg aagccagcca
     2161 gatgtcgccc gtgcgcaatg caatcctggc ggcgatactg cgccggtaaa atagccctgg
     2221 cttctgccgt cgagacttta atccccggat acgccagcac ccacagccac tcatcaaacc
     2281 ctggcacttg ctggctgatg atgtcgtttt cttcgatcat cagctgcata ccaccaagaa
     2341 aacacggtgc cacgttgtcg taatgaatgc tgccggagat tcgtccttcc agctcgccca
     2401 tcaaagccag caaacgagtg tcattaagcg gcttgccaca gtgttcattc atcgccatca
     2461 gcgccgcgac caccgaacag gcgctggagc ctaagcccga accgattggc atattctttt
     2521 ccagagtcat cgccactgga atttgcttgc caagctcctg gcaaaaacgc tcccagcact
     2581 gataaacgat attttcccgt ggttctgacg gcagcttatc ggcaaagcgt ccgaggttgt
     2641 tgagactgaa tgtctctgcc gcctcaaccg tgactacatc tccgagcaat gcaccatcaa
     2701 caggtgtcac cgccgccccg agcacatcaa acccgacgct catattggca ctggaagccg
     2761 gggcataaac tttaaccatg tcagactcct aacttccatg agagggtacg tagcagatcg
     2821 gcaaagacac cggcagctgt aacgtcattg cccgcaccat atccgcgcag caccaacggc
     2881 agcggctgat aatagtggct ataaaaggcc agggcgtttt cgccattttt cactttgaac
     2941 agcggatcat taccatccac ttcggcaatc ttcacgcggc aggcaccatc ttcatcaata
     3001 ttgccaacat agcgcaaaac ttttccttca tcacgggcct tcgccacgcg cgcggcaaag
     3061 agatcgtcga gctgtgacag attcgccata aaagcggcaa catcaccctc agcgttaaac
     3121 tctgcgggca gcacaggttc aatttcaata tccgccagct ccagttcacg tcccgtttca
     3181 cgagcgagaa tcaatagctt acgcgctaca tccataccag aaagatcatc tcgcggatcc
     3241 ggttcggtat aacccatttc ccgcgccaga gtagtcgcct cggagaaact catgccttcg
     3301 tctaacttgc cgaagatata agaaagcgaa cctgaaagaa tgccggagaa cttcatcaat
     3361 tcatcaccag cattgagcag attttgcagg ttctcaataa ccggtaatcc agccccaacg
     3421 ttggtgtcat agaggaattt acgccgcgat ttttccgccg catgacgcaa cagatggtag
     3481 taatccatcg acgaggtgtt ggcctttttg ttcggcgtga caacgtggaa accttcgcgc
     3541 aagaagtcgg catattgatc cgccactgcc tggctggagg tgcagtcaac aatgaccggg
     3601 ttcagcagat gatattcttt cacgaggcga attaagcgcc cgagattaaa cggctctttg
     3661 gcttgcgcca gttcttcctg ccagttttcc agatttaggc catgcacatt ggtgagcagt
     3721 gccttcgagt tggcaacacc gcagacacgt aagtcgatat gtttattctt cagccagctt
     3781 tgttgacgct tcagttgctc cagcagcgca ccgccaacgc caccgacgcc aatcacaaac
     3841 acttcgataa cctgatcggt attgaacagc atctgatgag taacgcgcac gccagtggtc
     3901 gcatcatcgt tatttaccac gacagagatt gagcgttcag aagatccctg agcaatggcg
     3961 acaatgttga tattggcgcg ggccagcgcg gcaaagaatt tcgccgagat cccacgcaag
     4021 gtgcgcatac catcacctac caccgagata atggccagcc gttccgtcac cgccagcggc
     4081 tccagtaagc cttctttcag ttccaggtag aactcttcct gcattgcccg ttcagctcgc
     4141 acacagtcgc tttgtggaac gcagaaactg atactgtatt cggaagatga ttgcgtaatc
     4201 agcaccacgg aaatacgggc gcgtgacatc gcggcaaaga cgcgcgccgc catgccgacc
     4261 attcctttca tccccgggcc ggaaacgctg aacattgcca tgttattcag attggaaatg
     4321 cccttgaccg gtaattcgtc ttcatcacga ctggcaccaa tgagcgtacc tggtgcttga
     4381 ggatttccgg tatttttaat caggcaaggg atctggaact gggcgatggg ggtaatggtg
     4441 cgggggtgaa gaactttagc gccgaagtag gaaagctcca tcgcttcctg gtaggacatc
     4501 gacttcaaca acctcgcatc gggcacctga cgcgggtcgc aggtatagac cccgtcaacg
     4561 tccgtccaaa tctcgcaaca atcggcgcgt aaacaggcag ccagcaccgc cgcggagtag
     4621 tcggaaccgt tgcgtccaag taccaccagt tcgccttttt cattaccggc ggtgaaacct
     4681 gccatcaaca ccatgtgatc agccggaatg cggcttgccg caatacggcg ggtggactca
     4741 gcaatatcga cggtagattc gaggtaatgc cccactgcca gtagtttttc gaccggatcg
     4801 ataacggtaa cgttgtgacc acgcgcttct aacacgccgg ccataatggc gatcgacatt
     4861 ttctcgccac ggcaaatcag cgcagcgttg atgctatccg ggcactgccc caacaaacta
     4921 atgccatgca ggacatgttt tatttgggca aattcctgat cgacgaaagt tttcaattgc
     4981 gccagcggga accccggctg ggcggcggcg agtcccgtca aaagttcggc aaaaatacgt
     5041 tcggcatcgc tgatattggg taaagcatcc tggccgctaa tggttttttc aatcatcgcc
     5101 accaggtggt tggtgatttt ggcgggggca gagaggacgg tggccacctg cccctgcctg
     5161 gcattgcttt ccagaatatc ggcaacacgc agaaaacgtt ctgcatttgc cactgatgta
     5221 ccgccgaact tcaacactcg catggttgtt acctcgttac ctttggtcga aaaaaaagcc
     5281 cgcactgtca ggtgcgggct tttttctgtg tttcctgtac gcgtcagccc gcaccgttac
     5341 ctgtggtaat ggtgatggtg gtggtaatgg tggtgctaat gcgtttcatg gatgttgtgt
     5401 actctgtaat ttttatctgt ctgtgcgcta tgcctatatt ggttaaagta tttagtgacc
     5461 taagtcaata aaattttaat ttactcacgg caggtaacca gttcagaagc tgctatcaga
     5521 cactcttttt ttaatccaca cagagacata ttgcccgttg cagtcagaat gaaaagctga
     5581 aaaatactta ctaaggcgtt ttttatttgg tgatattttt ttcaatatca tgcagcaaac
     5641 ggtgcaacat tgccgtgtct cgttgctcta aaagccccag gcgttgctgt aaccagtcga
     5701 ccagttttat gtcatctgcc actgccagag tcgtcagcaa tgccatgact cgttcgcgta
     5761 aagcttgcag ttgatgttgg tctgccgttg tgtcactttt cgtcggttgt tgtattaatg
     5821 ttgctaattg atagcaatag accatcactg cctgccccag attgagcgaa ggataatccg
     5881 ccaccatcgg cacaccagta agaacgtcag ccaacgctaa ctcttcgtta gtcagcccgg
     5941 aatcttcgcg accaaacacc agcgcggcat ggctcatcca tgaagatttt tcctctaaca
     6001 gcggcaccag ttctactggc gtggcgtagt aatgatattt cgcccgactg cgcgcagtgg
     6061 tggcgacagt gaaatcgaca tcgtgtaacg attcaaccag tgtcgggaaa actttaatat
     6121 tatcaataat atcaccagat ccatgtgcga cccagcgagt ggctggctcc agatgtgcct
     6181 gactatcgac aatccgcagt tcgctaaacc ccatcgtttt cattgcccgc gccgctgccc
     6241 caatattttc tgctctggcg ggtgcgacca gaataatcgt tatacgcata ttgccactct
     6301 tcttgaccaa atgaccgcga acagggtgat cactgtcaac ttattacgcg gtgcgaattt
     6361 acaaattctt aacgtaagtc gcagaaaaag ccctttactt agcttaaaaa aggctaaact
     6421 atttcctgac tgtactaacg gtttaattgt taaaaaatgc tacatatcct tctgtttact
     6481 taggataatt ttataaaaaa taaatctcga caattggatt caccacgttt attagttgta
     6541 tgatgcaact agttggatta ttaaaataat gtgacgaaag ctagcattta gatacgatga
     6601 tttcatcaaa ctgttaacgt gctacaattg aacttgatat atgtcaacga agcgtagttt
     6661 tattgggtgt ccggcccctc ttagcctgtt atgttgctgt taaaatggtt aggatgacag
     6721 ccgtttttga cactgtcggg tcctgaggga aagtacccac gaccaagcta atgatgttgt
     6781 tgacgttgat ggaaagtgca tcaagaacgc aattacgtac tttagtcatg ttacgccgat
     6841 catgttaatt tgcagcatgc atcaggcagg tcagggactt ttgtacttcc tgtttcgatt
     6901 tagttggcaa tttaggtagc aaacatgcag accccgcaca ttcttatcgt tgaagacgag
     6961 ttggtaacac gcaacacgtt gaaaagtatt ttcgaagcgg aaggctatga tgttttcgaa
     7021 gcgacagatg gcgcggaaat gcatcagatc ctctctgaat atgacatcaa cctggtgatc
     7081 atggatatca atctgccggg taagaacggt cttctgttag cgcgtgaact gcgcgagcag
     7141 gcgaatgttg cgttgatgtt cctgactggc cgtgacaacg aagtcgataa aattctcggc
     7201 ctcgaaatcg gtgcagatga ctacatcacc aaaccgttca acccgcgtga actgacgatt
     7261 cgtgcacgca acctgctgtc ccgtaccatg aatctgggta ctgtcagcga agaacgtcgt
     7321 agcgttgaaa gctacaagtt caatggttgg gaactggaca tcaacagccg ttcgttgatc
     7381 ggccctgatg gcgagcagta caagctgccg cgcagcgagt tccgcgccat gcttcacttc
     7441 tgtgaaaacc caggcaaaat tcagtctcgt gctgaactgc tgaagaaaat gaccggccgt
     7501 gagctgaaac cacacgaccg tactgtagac gtgacgatcc gccgtattcg taaacatttc
     7561 gaatctacgc cggatacgcc ggaaatcatc gccaccatcc acggtgaagg ttatcgcttc
     7621 tgtggtgatc tggaagatta atcggcttta ccaccgtcaa aaaaaacggc gctttttagc
     7681 gccgttttta tttttcaacc ttatttccag atacgtaatt gatcgtccat tgtaacttct
     7741 ttactggctt tcattttcgg cagtgaaaac gcataccagt cgatattacg agtcacaaac
     7801 atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc gctatcggca
     7861 gagttgagca gtccccacat cacaccatcc agcaacaaca gcgcgagggt aaacaacatg
     7921 ctgttgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa cgccccaatc
     7981 agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc cagcaagagc
     8041 aaataaaaca ttaccaatga aagccccacc agcaaatatt gcattgggtg taaacgttgc
     8101 gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag cagaatggcg
     8161 tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac tgcgacgcta
     8221 aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc accgagatta
     8281 ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctaac ttcccgtttg
     8341 gctggcaaaa aatcacctaa aaaactggga tgcggccagt tgctggttaa ggtcatttcg
     8401 ctattacgcc cggcaggcac cacagaaaga tcgccggtac cgcttaaatt cagggccata
     8461 ttcagcttca ggttctgctt ccgccagtcc ccttcgggta aagggatatg cacgccctgc
     8521 ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt aacttcaggc
     8581 gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa gggcttgcct
     8641 aagatgatat ttggcgcatt gagctcgcta agacgcgaaa catcgaaatc ggcttttagc
     8701 gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc ttccacgttc
     8761 tgattgccat caaccatcaa tgactcaggt aaccaaaaat ggataaaact tcgtttccgc
     8821 tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat agcgataagc
     8881 ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc cacatcgcta
     8941 cggtaatcgg cacgttcgac aatcacctgc cgaatcagca ttatcggaat caacaacagc
     9001 aatactgcac caaacagggt ggtcattttc cagaacaggg gggatttcaa catagtcttc
     9061 tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag ctatgtgaag
     9121 tgacggtgaa gtcgaagcga ggccagcacg ccaccttcct gcacgttgcg cagcgtgact
     9181 tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc gctgcttttt
     9241 tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag cgcgtagtca
     9301 ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc cacttcggcg
     9361 cttagcgtta tacgaccgct ctcgggggta aaatcgatgg cgttatccag taaattcccc
     9421 agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggtggg cgtaacatgt
     9481 agagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg gcggaataat
     9541 gccgccacat caacagcagt caaaacgact tcctgacgat tctccagtct tgcctggcgt
     9601 agtaacgttt ccaccagtgc ctgcattcgc gcattttgcg tcagaatgtt gtcggtaaaa
     9661 cgagccacca cttccggtgg cggaccttcg cgtaaaattt ccgccgcgcc acgaatcgcc
     9721 gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat atagtttttc
     9781 ccttccagct tcacgcgcat actttccagc gcctgcgcaa gcttacgcaa ctcgctactg
     9841 ccgagttcag gaagaggaac gggcttattg tcagtgacgg aatcggcata gcgagtgagc
     9901 cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag tgcaatcccc
     9961 agcaaaatgg cgctggccca taaaattcgc cgctcgctac gcttaatgac cggagccatc
    10021 gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgtc cataatcggc
    10081 gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt gctgcgcgca
    10141 ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc ctgcccaacg
    10201 gctttatttg ccgaatcgaa caatacttta ccctgcgaat cggtcatata gacatgatat
    10261 tcattgcgca ctttgttaat gccaccgata ttggcgcgaa acgggcgatg ttgtagctga
    10321 ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc gggacgcgcc
    10381 agctccgcca gcaacgttgc ggtatcgatt aacgtcccct ccgttgctct tcgcacgccc
    10441 ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc caccagtaaa
    10501 aaatagccca gcagcaaccg catgccgata cgcattacag gcccctcagg ctatatccca
    10561 tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc ttggcacgca
    10621 acgttttaat gtgggtatca acggtgcgat cgtaggtgtc ctgcgcatct tcccacacgc
    10681 tatccatcag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac gtcttcagca
    10741 ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc tgcgccgcgg
    10801 gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgacgag aacttcttca
    10861 cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac ggtttagcca
    10921 catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca cttcgggccg
    10981 tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac aattcaaagc
    11041 cgctgatatc aggcaaacca acatcgagaa tcatgacgtc gggaacctgc tggcgagctt
    11101 tatccagcac cggcaagcct cgctcaaaga cctcgacggc aaaaccttcc tgctgcaaca
    11161 tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc gtttcccgtt
    11221 gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta atcgcgtttt
    11281 tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg ttgcgtttag
    11341 catcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtacgt tttttgaata
    11401 ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc ggcccgactt
    11461 gctgacaaga aatggccgca tcggaggtat cttccgccag gcccaatccc cctttaatac
    11521 caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga tcgtcaaagg
    11581 cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg accgaaccaa
    11641 tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg atcaaatgct
    11701 tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg tcacggtaaa
    11761 taacatattg attaagaaca aaatctcaat tcttttattt ccgtcaagcc ctaaaacata
    11821 ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta tccaattacc
    11881 tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg ccggcattat
    11941 tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc tcgacaatgt
    12001 agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag atgtcactgg
    12061 ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg tcgcactacg
    12121 cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact ctcaacagac
    12181 atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc gccgttctcc
    12241 tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca caatgccaga
    12301 gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga gctactcctg
    12361 ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct ggcacgattt
    12421 tctcggcaac gcgccgacca ttccgccggt gctctacggc ctgaatgaaa cgcgtccgag
    12481 tcaggataaa gacgacgaac aagaggtatt ctataccacc gcgttagccc aggatcaggc
    12541 agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat atgtgatgtt
    12601 tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat acggaacgtg
    12661 catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat actacccggc
    12721 agaagatgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc tgattccgat
    12781 ccgtcgttaa cgctgcaact catctaatgc aggggtgtcc agatgcgaga tgtcccctgc
    12841 ggtttcaaca acccagccag acgccagcca caggctttcc tgataatcca cgcgcgaaat
    12901 agaacagtta cgcagacgta agcggcgctc tgcccatgct ggtaatccga gaatcgtact
    12961 caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc cctgcggtaa
    13021 gtcccggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca ttgactcgcc
    13081 ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt tctcttcttc
    13141 ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt cacgcaggcg
    13201 agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg tacgccgggt
    13261 gcgtcctaaa tcgctactga taatatgcgt aatgccaagc tctttggcac gggttgccac
    13321 ctgcatcgcc tgttgctcac ctttggcggt cagagggctg tcagactggc cctgaatacg
    13381 tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta acatgctttt
    13441 tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca ccaagttgtc
    13501 tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca cgagatcttc
    13561 ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc ggaacaaccc
    13621 tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc ccgccgttta
    13681 cttccagagg ctgatttttg ggtggcgatt gaagctggca tcgatggtga cagcactttc
    13741 agttgggtgg tgattgaaaa caccagccag cgcggcgagg cgcgttcggc tactttgccg
    13801 ttacctgcgg tgattctgca gaaagtgcgt gaaggtgagg cgcttggtcc cgtgatgtcg
    13861 cgttataccg gtattgatga gattggtcgt aaggaagggg cgatcggcgt gtttaccgcc
    13921 gggaaactca ctcgcgccag cgtctatcac caggcggtga tcctggcgct gagtccgttt
    13981 cacaatgccg tgtatcaggc attgtaggac ggataaggcg ttcacgccgc atccggcatt
    14041 cggtgcacga tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa atcaatcgct
    14101 tttcagcaac acctcttcca gccactggcg cagctccacg ggcgcggctt tcaggctgtt
    14161 agatccacgc gtaatcgtcg cgatgcccgc gccgagttca ttttttaact cacgctggct
    14221 catttcgccg cgcaacagct cttcgacaat acgcacgcga gtccccaacg cttcgcgctc
    14281 atctggcgtc agcatcagat ttaacaacgg taaatggaga tcgttttggt aggcattctt
    14341 aagcaggtcg acaaaacgta accactcctg gtgacgctgt tctgccatcg ctgctgaata
    14401 gggtgattgt tgggccataa tatgtcgcca ttgttagcgg gggaagcaaa atgcctcccc
    14461 cggttgtact cgctaaagag tacgatagca tatcataaac gtgcggatca gtaacgacgt
    14521 ccccattccg tggcgctcat caacgtcggt ttatccccca tgaaatagcg gtagtaagcg
    14581 tcataagcca gcacgttctt cacataaccg cgcgtctcgg agaacggaat actctcgaca
    14641 aatgccacag cgtcgatacg cccggcgctg ttgccaagcc aggttcgcac ccgccctggt
    14701 ccggcgttat aagctgctga ggagaaaata cggttattgc caaactgctg ataaacatat
    14761 tgcagataac tggtgccaat gttgatattc gtttccggat ccagcaattg cccagggcta
    14821 ctataaccgg gaatagagaa catcttcacc gtatgggtcg ctgtaccagg cataatctgc
    14881 atcaggccgc tggcccctac cggtgatttc acttttggat tccaggcgct ctcctgacgg
    14941 gcaatcgcca tcgcatagct ttgcgggatc tccttaccgc tggtgtagcg tttgaaaaga
    15001 tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc atagcttccc ggcgatcgtt
    15061 gcctgaacgc taagatccca ccattggttg ttgaaagcat accgcgccag ttgagcctgc
    15121 tctgtttttg acttgctctt caccagattg gcccactcgc tacgcgcggt gttatcgaga
    15181 ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc cctgagtcag ggcgctgtca
    15241 acattctgcg gcgctttatc aatcttcagc tcatactctt ctccgatgcg ttgtgctgca
    15301 accatcgggt agaaaccacg ctgttgcatg agttgatgca aaatctcttt cgcttcagct
    15361 tcacgtccgc gttccagcaa taaatccgcc tgccagtaac gccattcatc tttctctttt
    15421 gcttccatcg gcagacgcgc cagccaggta ttcaggccgc gacgatcgcc ggtgccaagt
    15481 gccattcgca cacggcgttc aatcaacgac gtggattgcg agcgcataat ggcgtcatcg
    15541 cgccatttcg cctgcttgtc ggtgacatcg ttgcccatca aacgccaggc gacgatatcg
    15601 cgcagctcct gaatctgatc ttcattaagc tgctgcgcct gggcaagcga cgggatcatc
    15661 agccgtgcat tttcagcatc ctgccgcgcc acactggcaa acgccaccgc tgccatttga
    15721 cgggtaaaat cggtcgctcc ggttgtacgc acgaaggtca gtaccgtatt agggttgttc
    15781 gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg caggcatctg ccctgccagc
    15841 actgttacca ggccagtgtt acccgctttc atcgccagac ggatacgctc taaatacgcc
    15901 agcggatctt gtttacctga cgcacgccag acgctaaata acttgtcaca ggcgttaggc
    15961 tggctcttgc cggttagcca cagctctttc gccccttgcc aggcttcttc actctgcccg
    16021 gtgttccatt tcgcatagta gtaattacat tgcgcttcgg tagttccggg cttttccggg
    16081 ctaaaggcta acaagccacg ccagtcttca cgccgcgcca gttcattgac gaaacgagat
    16141 tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa caaagttagt gaccgtcacc
    16201 gccggttgat tcatcagatc atcggtgatc tggcggtatt ccaggtaggg ataaagcgga
    16261 taatccttca gtccaggcat catttgttcg accacatcca tttgtcgatt atcccaggcc
    16321 tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg agtcggctcg cgccacgctg
    16381 ctgaccgtca gcagacagac accggcagcc aacagccgcc aggtaacttg tttggctttt
    16441 tccacaagcg catcctctaa gtgtaaatac atcaatgcag cgtcatgccg cgtaatagat
    16501 ttatgctaac cagtcattgc cgtttacgcc acgttacgga cactttttta cttttactgc
    16561 gaaggtgatc gaccaactgg gattaggcgg ggaaaaaggc tacacttcgc ctttgaaaac
    16621 tcatcatcac gataaacata gaggcgaagt ccaacgtggc tcaattcgtt tataccatgc
    16681 atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt gaaaaacatc tctctgagtt
    16741 tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg cgcgggtaag tctaccctgc
    16801 tgcgcattat ggcgggcatt gataaagaca tcgaaggtga agcgcgtccg cagccagaca
    16861 tcaagattgg ttacctgccg caggaaccgc agctgaaccc ggaacacacc gtgcgtgagt
    16921 ccattgaaga agcggtttct gaagtggtta acgccctgaa acgcctggat gaagtgtatg
    16981 cgctgtacgc cgatccggat gccgattttg acaagctggc cgctgaacaa ggccgtctgg
    17041 aagagatcat tcaggctcac gacggtcata acctgaacgt acagctggag cgtgcggcgg
    17101 atgcgctgcg tctgccagat tgggacgcga aaatcgctaa cctctccggt ggtgagcgtc
    17161 gtcgcgtggc gttgtgccgc ctgcttctgg aaaaaccaga catgctgctg ctcgacgaac
    17221 cgaccaacca cctggatgcc gaatccgtgg cctggctgga acgcttcctg cacgacttcg
    17281 aaggcaccgt tgtggcgatt actcacgacc gttacttcct cgataacgtt gcaggctgga
    17341 tcctcgaact tgaccgcggt gaaggtattc cgtgggaagg caactactcc tcctggctgg
    17401 agcagaaaga tcagcgcctg gcgcaggaag cttcacaaga agcggcgcgt cgtaagtcga
    17461 tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg ccgccagtcg aaaggtaaag
    17521 cacgtctggc acgctttgaa gagctgaaca gcaccgaata tcagaaacgt aacgaaacca
    17581 acgaactgtt tattccacct ggaccgcgtc tgggcgataa agtgctggaa gtcagcaacc
    17641 tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct gagcttctcg atcccgaaag
    17701 gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa atcgaccctg ttccgtatga
    17761 tctccggtca ggaacagccg gacagcggca ccatcacttt aggtgaaacg gtgaaactgg
    17821 cgtcggttga tcagttccgt gactcaatgg ataacagcaa aaccgtttgg gaagaagttt
    17881 ccggcgggct ggatattatg aagatcggca acaccgagat gccaagccgc gcctacgttg
    17941 gccgctttaa ctttaaaggg gttgatcagg gtaaacgcgt tggtgaactt tccggtggtg
    18001 agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg cggcaacatg ctgctgctcg
    18061 acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc gctggaaaac gccctgctgg
    18121 agttcccggg ctgtgcgatg gttatctcgc acgaccgttg gttcctcgac cgtatcgcca
    18181 cgcacatcct ggactaccag gatgaaggta aagttgagtt cttcgaaggt aactttactg
    18241 agtacgaaga gtacaagaaa cgcacgctgg gcgcagacgc actggagccg aagcgtatca
    18301 agtacaagcg tattgcgaag taatgcgtaa aatgccggat gcggcgtgaa cgccttatcc
    18361 ggcctacaaa ccaagaaatt tcaatacgtt gtgaatctcc aggcctgata agacgcggta
    18421 agcgtcgcat caggcattga gcaccgaatg ccggatacgg cgtgaacgcc ttttccggcc
    18481 tacaacagcc ttagtatccg caaattcgta gcgggtcacc cgtgctgatt cttttaaacc
    18541 ttgtttaatc tcgacagtct cttccagaaa ggttttcaac tcatcaaaaa acttatttta
    18601 tacctcgcct ttgagccgtt tcatcgcggt tatctctgct cccccatcat ctcccgcacc
    18661 agctccacgc agcgcaggaa acggctgtca taatcgtcct cttcaacccg cacgaattcg
    18721 atattgttct cttccagcat ctccaccagc aagttctgga actctttgcg atccaccgaa
    18781 ctgccgaggc tgcgtaaacc atccgccacc cacggcgtgt tgttctccag caggatcacc
    18841 agatcgaaac ggtattcatc aatcagcgcc tgcacgaacg gatgctcacg cccttcgtac
    18901 tttttgcaga acgcctgggt agtgacaaaa tcagtgtcaa taaacgccac tttattggca
    18961 tatttcactg caaaatcaat atattgtgcg tgacccagcg cgattttatc gtagtcggaa
    19021 tactgcaatg cgatctcatc accgccgagg tgtgaaaaga cataatcgcg accatattcc
    19081 cacgcactgg tggtattaaa gatattggca agtttgttta ccagggtgga tttaccgctc
    19141 gactcgccgc caaggatcgc cacggtacgc acaaagaacg gcttcacttc cgtaggaata
    19201 tattcccagt agcggaacgg gttttcgcgg atctgtgcac cgctgatact cataaaggta
    19261 cgtttcggat cgaccagcac cgtctcgatc cccagatgtt ccatatactg tggcgcatcg
    19321 gcttcctccg aggtgtagat caggtccggc tggatcccct tttcagccat aaactttttg
    19381 atgccgttgc tccacacatc ccagccgtgc ggatacggct ccatgccctc ttcgttgaaa
    19441 gcatgaatgc gaatattttt ctgatattta aaggtttgta ataaccagcg cagacgatcc
    19501 ggcaccgtgg gctgctgcga catggcgctg tcttcaaaca acgcgcggtc gcgggtatcg
    19561 tcaaaaccca taatgatatg cagctcgtca acctggctac aggcgcgctg gataaggtag
    19621 atatgtccgg tatgcagcgg gtagaactta ccgaatacga caccaatggt tttcttctgt
    19681 cggggaaatt caagccccaa aaaacggtgc aacgcctcca gcttttgcgc gctggggctt
    19741 ttaattttgg cattcagtaa ctggcttaaa taccctttgg tcataccgct ggcatcagct
    19801 acctgctgta gcgtgcagcc ctgttgcttg atggcagttt tcaggtaatc aaatgacgac
    19861 atatctccct ccgtatctct cattataagt cgtcgaacac gctaagcgcg tcggagagtt
    19921 ttttaacgcc aaaaatctgc atcccttccg gcgctttttt cggtacgtta gccgccggaa
    19981 caatcgcccg gcgaaaaccg tgtttcgccg cttctgaaat tcgttcctga ccgctgggca
    20041 ccgggcggat ctcccctgcc agcccgactt caccaaacac caccagatcc tgtggcagcg
    20101 gtctgtcacg caggctggaa accatcgcca gcagtaacgc taaatcggca ctggtttcgg
    20161 ttaccttcac gccgccgacc acgttcacaa atacatcctg atcggccatt tgcagaccac
    20221 cgtgacggtg caagacagcc agcaggattg ccagacggtt ttgttccagc cccactgcca
    20281 cgcggcgcgg attcgccatc atcgagtgat cgaccagcgc ctgaatctcc accagcaacg
    20341 gacgcgttcc ttcccacacc accatcacgg agctgccgga ggtcacttca tctccgcgac
    20401 ttaagaaaat tgccgaaggg ttgctgactt cacgcagccc ctgttcggtc atcgcgaaga
    20461 cgcccagctc attcaccgcg ccgaagcgat ttttatggct gcgcaaggtg cgaaaacggg
    20521 agtcggcatc accatccaga agcaccgaac agtcgataca gtgttccagc actttagggc
    20581 cagccagcga gccatctttg gttacgtgcc caaccatgac aatcgccaca ccgcgcgttt
    20641 tggcgaagcg cgtcagataa gccgccgttt cacgcacctg cgccacgcta ccaggcgatg
    20701 attgtacatc cgccatatgc atcacctgga tcgagtcaat taccatcagt ttcggttgct
    20761 cttcttcggc aatcaggcag atctgttcga tgctggtttc cgacaacata ttgagattgt
    20821 cagtcggcag gccaaggcga tgagcgcgca ttgccacctg ttgcagcgac tcttcgccag
    20881 tgacatacag cgttttcatc tgctgggcca gtttgcacag cgtttgcagt agcagcgtgg
    20941 atttccccgc acctgggtta ccgccaatca gaatggcgct tcctggcacc acgccgccgc
    21001 ctagtacgcg gtcgaactct ttaaatccgg tggaaaaacg cggcagctct tcaaggctga
    21061 tatcggagag tttctggact tttgccaccc cggcgctacc ggcatagccg ctgagacgct
    21121 cgttacgcgc caccgttggc gacgcagcaa gacgcacctc ggtgatggtg ttccaggcat
    21181 gacaggcact gcactgcccc tgccagcgcg gataatcggc cccgcattca ttacaaacaa
    21241 aggcgcgttt tggagctttt gccacggtgt acctcgttaa tgctgtgccg cccgcaggat
    21301 ggcgggcgag caattacttc tgattcaggc tgcctgagag gatgcagaat acccccatca
    21361 ggtcagcgtg acggatggtg acttccgcct tttcattcac ttttggcttg gcatggtagg
    21421 caatccccag ccctgctgct ttgatcatcg gcaggtcatt agctccatcg ccaatcgcca
    21481 cggtctgcgc cagcgggatt tcatactcct gcgcgaggcg agtcaaagtt ttcgctttgt
    21541 actgcgcgtc tacgatgtcg ccgatcacat tgccggtaaa tttaccgtcc atgatctcca
    21601 gttcattggc taccacggcg gtcaggcgca gcttgtcgcg cagatattca gcaaagaaag
    21661 taaagccgcc ggaggcaatc gccactttcc agcccagcgt ttccagcttg agcaccagtt
    21721 gcgttaagcc tggcatcagc ggcagatttt cacgcacctg ttgcagaata ttggcgtcag
    21781 cgcctttcag cgtcgccaca cggctgcgca ggctggcggt aaaatcgagt tcgccgcgca
    21841 tcgcccgttc ggttacttcc gccaccatct cgcccgttcc ggccagtttg gcaatttcat
    21901 caatacattc aatctggatg gcggtggaat ccatgtccat caccagcaaa cccggcgtgc
    21961 gcaggtgcgg gattttcccc agcggggcga catccagttg cgcttcatgg gccaggcgcg
    22021 tagcccgtgc ggtgagtgaa cctgccagac gaatcacctg ataatcttcc acgcaccagg
    22081 cggcaacaat caccatcgcc gcacccagtt tgctctggta ttgggtcaga cgctgtttat
    22141 ccagcccacg accatacagc agccagccgc tacgacctgc gtggtaatcc agtggcatca
    22201 cttcatcacc acttaatgaa agaggcagac caggccataa agagacatct tcaggcaggt
    22261 cgcaccaggt aatgttaggc attaaggctc ctgtaaaatc gttcgaagca gggaaaataa
    22321 cgcatgaggc taccttgtat ccattgcttc tggcaacatt aagtctcaaa ttttcaaagg
    22381 gtggaagatg gctcgcacaa aactgaaatt ccggctgcat cgggcagtga ttgtcctgtt
    22441 ctgtcttgcc ttgttagtgg cgctgatgca gggagcgtca tggtttagtc aaaaccacca
    22501 gcgacaacgt aatccacagc tggaagaact ggcccgcacc ctggcgcgtc aggtaacgct
    22561 gaacgttgca ccgctgatgc gtaccgactc accggatgaa aaacgcattc aggcgatcct
    22621 cgatcagtta acggatgaaa gccgtatcct cgacgcgggt gtgtatgacg aacaaggcga
    22681 tcttatcgcc cgttctggcg aaagcgtcga agtgcgcgac cggctggcgc tcgacggtaa
    22741 aaaagcgggc ggctatttta accagcagat tgtcgaacca attgccggga aaaacggacc
    22801 gctcggctat ctgcgcctga cgctcgacac ccatacgctc gccaccgaag cccaacaggt
    22861 ggataacacc actaacattt tacgcctgat gttgctgctt tcactggcaa tcggcgtagt
    22921 gctgacccgc acgct
//
LOCUS       DAEVZA010000078        22714 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEVZA010000078 DAEVZA010000000
VERSION     DAEVZA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.078"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(260..336)
                     /locus_tag="LAF92_003012"
     tRNA            complement(260..336)
                     /locus_tag="LAF92_003012"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(340..432)
                     /locus_tag="LAF92_003013"
     tRNA            complement(340..432)
                     /locus_tag="LAF92_003013"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Ser,seq:gct)
     gene            complement(748..933)
                     /gene="csrA"
                     /locus_tag="LAF92_003014"
     CDS             complement(748..933)
                     /gene="csrA"
                     /locus_tag="LAF92_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBI9791981.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1168..3798)
                     /gene="alaS"
                     /locus_tag="LAF92_003015"
     CDS             complement(1168..3798)
                     /gene="alaS"
                     /locus_tag="LAF92_003015"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBI9791982.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3926..4426)
                     /gene="recX"
                     /locus_tag="LAF92_003016"
     CDS             complement(3926..4426)
                     /gene="recX"
                     /locus_tag="LAF92_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBI9791983.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4669..5730)
                     /gene="recA"
                     /locus_tag="LAF92_003017"
     CDS             complement(4669..5730)
                     /gene="recA"
                     /locus_tag="LAF92_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBI9791984.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5810..6307)
                     /gene="pncC"
                     /locus_tag="LAF92_003018"
     CDS             complement(5810..6307)
                     /gene="pncC"
                     /locus_tag="LAF92_003018"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBI9791985.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6452..7537)
                     /gene="mltB"
                     /locus_tag="LAF92_003019"
     CDS             complement(6452..7537)
                     /gene="mltB"
                     /locus_tag="LAF92_003019"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBI9791986.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7793..8356
                     /gene="srlA"
                     /locus_tag="LAF92_003020"
                     /gene_synonym="gutA"
     CDS             7793..8356
                     /gene="srlA"
                     /locus_tag="LAF92_003020"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBI9791987.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8353..9312
                     /gene="srlE"
                     /locus_tag="LAF92_003021"
                     /gene_synonym="gutE"
     CDS             8353..9312
                     /gene="srlE"
                     /locus_tag="LAF92_003021"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBI9791988.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9323..9694
                     /gene="srlB"
                     /locus_tag="LAF92_003022"
                     /gene_synonym="gutB"
     CDS             9323..9694
                     /gene="srlB"
                     /locus_tag="LAF92_003022"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBI9791989.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9698..10477
                     /gene="srlD"
                     /locus_tag="LAF92_003023"
     CDS             9698..10477
                     /gene="srlD"
                     /locus_tag="LAF92_003023"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBI9791990.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10582..10941
                     /gene="gutM"
                     /locus_tag="LAF92_003024"
     CDS             10582..10941
                     /gene="gutM"
                     /locus_tag="LAF92_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBI9791991.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11008..11781
                     /gene="srlR"
                     /locus_tag="LAF92_003025"
                     /gene_synonym="gutR"
     CDS             11008..11781
                     /gene="srlR"
                     /locus_tag="LAF92_003025"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBI9791992.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11774..12739
                     /gene="gutQ"
                     /locus_tag="LAF92_003026"
     CDS             11774..12739
                     /gene="gutQ"
                     /locus_tag="LAF92_003026"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBI9791993.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12736..14250)
                     /gene="norR"
                     /locus_tag="LAF92_003027"
     CDS             complement(12736..14250)
                     /gene="norR"
                     /locus_tag="LAF92_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBI9791994.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14437..15876
                     /gene="norV"
                     /locus_tag="LAF92_003028"
     CDS             14437..15876
                     /gene="norV"
                     /locus_tag="LAF92_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBI9791995.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            15873..17006
                     /gene="norW"
                     /locus_tag="LAF92_003029"
     CDS             15873..17006
                     /gene="norW"
                     /locus_tag="LAF92_003029"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBI9791996.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17232..19484)
                     /gene="hypF"
                     /locus_tag="LAF92_003030"
     CDS             complement(17232..19484)
                     /gene="hypF"
                     /locus_tag="LAF92_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBI9791997.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSTAVATLRARKHRPAKPLAVILPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19637..20164)
                     /gene="hydN"
                     /locus_tag="LAF92_003031"
     CDS             complement(19637..20164)
                     /gene="hydN"
                     /locus_tag="LAF92_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBI9791998.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20313..21323)
                     /gene="ascG"
                     /locus_tag="LAF92_003032"
     CDS             complement(20313..21323)
                     /gene="ascG"
                     /locus_tag="LAF92_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBI9791999.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21583..>22714
                     /gene="ascF"
                     /locus_tag="LAF92_003033"
     CDS             21583..>22714
                     /gene="ascF"
                     /locus_tag="LAF92_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBI9792000.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPA"
BASE COUNT         5307 a         6301 c         5795 g         5311 t
ORIGIN      
        1 gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat
       61 gcatcgggaa acttacttta ctgcagattt tttgataccg ctactaaagc cgtatcaagt
      121 aagagatggt gcatccggga ggattactcg ccttgcggct cgcccttcgg gccgttgcta
      181 aagcaacgtt atcctccctg gtgcctgcgg ttataccgcc acctcttcca taccttcatc
      241 gctgaaagta cgtagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
      301 gtagccgagt actctatcca gctgagctac ggatgcaaat ggcggtgagg cggggattcg
      361 aaccccggat gcagcttttg accgcatact cccttagcag gggagcgcct tcagcctctc
      421 ggccacctca ccacacgcct cttacgagtg cttcgaagaa cttgtttatc gctcatcgtc
      481 gctgcgtggc gcacatatta ctttctgaga cttataagtc aaacaatttt tcctacactt
      541 ttatcgtttg cacacttcac gttcaattag tctgtaaaaa cgtcaaaaag agtgttttat
      601 caacagaaaa atggaggtct gacagatagt agtaatgcaa aaaaatggag acttaagttg
      661 aatgaacggg agtaaagcga aaagactata gagtgaagga gaaattttga gggtgcgtct
      721 caccgataaa gatgagacgc ggaaagatta gtaactggac tgctgggatt tttcagcctg
      781 gatacgctgg tagatctctt cacggtgaac agaaacttcc ttcggggcat ttacgccaat
      841 acgtacctgg ttgcccttta cccctaaaac tgtcacggtg acctcatccc caatcatgag
      901 ggtctcacca actcgacgag tcagaatcag cattctttgc tccttgaaag attaaaagag
      961 tcgggtctct ctgtatcccg gcattatcca tcatataacg ccaaaaagta agcaatgaca
     1021 aacacattac atgtaagcag tcatggcatt acattctgtt aaacctaagt ttagccgata
     1081 tacacaactt caacctgact ttatcgttgt cgatagcgtt aatgcgaatg ccgtgaagcg
     1141 agtccacggc attgcctgac gcttatatta ttgcaatttc gcgctgaccc agcctttcac
     1201 actggctaac gctgcaggta aggccgcagc atccgtacca ccggcttgcg ccatgtcagg
     1261 acgtccacca cccttgccgc ccacctgctg agcgaccata ccaatcagtt cccctgcttt
     1321 cacacgatct gtgacgtcct tagatacgcc tgcaatcaga gaaaccttac cttcggctac
     1381 cgttgccagc acgataattg tcgaccccag ctgatttttt aaatcgtcaa ccatggtacg
     1441 caacattttc ggctcaacac cgctaagctc gctaaccaac agcttaacac cattaacatc
     1501 aattgcctta ctggaaagat ttgcgctctc ctgtgcggca gcttgttctt taagctgttg
     1561 tagttctttt tctaactgac gcgtacgttc cagtactgag cgcactttat ccgccagatt
     1621 attgctatcg cctttcagca gatgcgcgac ttcgcttaag cgatcgctgt ctgcatgaac
     1681 ggtggtgata gcgccttctc cggttaccgc ttcgatacga cgaacgcctg cagcagtacc
     1741 cgattcagag atgatgcgga acagaccaat atcaccagtg cggctggcgt gagtaccgcc
     1801 acacagctcg gtggagaaat cgcccatgct cagcacgcgt acacgctcat catacttctc
     1861 gccgaacagc gccatcgcac ctttcgcttt cgccgcttcg agatccatga tgttggtttc
     1921 gatcggcaaa ttgcgacgaa tctgtgcgtt caccaggtct tcgaccgcac gaatctcttc
     1981 cggtttcatc gcttcgttgt gtgagaagtc gaagcgcagc accttgtcgt taaccagtga
     2041 acctttctgc gatacatgag tccccagaac ctggcgcagc gcagcgtgca tcaggtgcgt
     2101 tgcggagtga ttcagacgaa tacgggcgcg acgagcctca tcaacatcag cctgcacagc
     2161 gtcgcccact ttcagagaac ccgcagcaag tttaccgatg tgaccaatcg cctggccgta
     2221 tttctgcgta tcttccaccg cgaaggagaa gttagcgcct ttcagttcgc ctttatcacc
     2281 aacctgaccg ccggattccg catagaatgg tgtttgatcc agcacgacca cagcttcctg
     2341 gcctgcattg atggcatcaa ccgctttacc atcaacaaac agcgcggtca ctttgccgtt
     2401 cagttccaga tggtcatagc ctttaaattc agatgcacta tcaacacgga tcattgcgtt
     2461 gtaatcggca ccaaagccgc tggcttcgcg cgcgcgacga cgctgctctt ccattgctgc
     2521 ttcaaaacca gcttcgtcaa ctttgatgtt gcgctcacga caaacatcag ccgtcaggtc
     2581 aaccgggaag ccataggtgt cgtacagacg gaaagccgtt tcaccatcca gcgtatcacc
     2641 agaaagtttt gccagctctt catccagcaa cgccagaccg cgctccagag tacgggcaaa
     2701 ctgctcttct tcagtcttca gcacctgctc aacctgcgcc tgctggcgtt tcaggtcttc
     2761 acccgcagag cccataacgt cgatcagcgg accaaccagt ttgtagaaga aggtttcttt
     2821 cgcgccgagc atattgccgt gacgcactgc gcgacgaatg atacgacgca gtacataacc
     2881 acggttttca ttggacggca ttacgccatc cgcgatcagg aacgcacaag aacgaatgtg
     2941 gtcagcgatt acgcgcagcg atttattgct cagatcggtc gcgccagtga ctttcgctac
     3001 cgcctggatc aacgtgcgga acaggtcgat gtcatagtta gagttaacgt gttgcagcac
     3061 cgcagcaata cgctccagac ccataccggt atctacagac ggcttcggca gcggttccat
     3121 cgtgccatct gcctggcggt tgaactgcat gaagacgatg ttccagatct caatgtagcg
     3181 gtcgccgtct tcttccggac ttcccggagg gccaccccaa atgtggtcgc cgtgatcgta
     3241 gaagatttcg gtgcacgggc cgcacggacc agtgtcaccc atctgccaga agttgtcaga
     3301 tgcgtatggc gcacctttgt tatcgccgat gcgaataata cgttcgcgcg ggatcccgac
     3361 ttctttttcc cagatttcgt aggcttcgtc gtcgctttca tagacggtaa cccacagacg
     3421 ctctttcggc agggcaaacc atttttcgct ggtcagcagt tcccatgcaa actgaatggc
     3481 atcgtgtttg aaatagtcgc cgaagctgaa gttgcccagc atttcgaaga aggtatggtg
     3541 acgcgcggtg taaccgacgt tttccaggtc gttgtgttta ccacccgcac gcacgcagcg
     3601 ttgggaagtg gtagcgcggg aataattacg cttgtcgagc ccaaggaaca catccttgaa
     3661 ctggttcatc ccggcgttgg taaacaacaa agttgggtcg ttatggggta ccagggagct
     3721 gctggcaact acctgatgtc ccttactatg gaaaaagtcg agaaacgcct gacggatctc
     3781 agcggtgctc ttgctcataa ttatcctgaa atcaagctaa cgaaatatcg ccaccagctc
     3841 cagcgtgtct taaccgccgg gctggtaact gaaaagtggg aataagataa gttttcttga
     3901 ctgggaagta aaataccgta tgcgttcagt cggcaaaatt tcgccaaata tcctggatat
     3961 cttccatcag atagccacga tagagcagaa aacgctggat cttaactttt tctgaaaaga
     4021 cagttggcaa aggttcacca tattttcgcg tcgcctgatc gcgcgccagt gcgcaccagt
     4081 cgatgtcaca ttcacgcatc gctttttctg tcgcttcgcg ggaaatacct ttctgattca
     4141 gttcctgacg aatacgtgca ggtccataac ctttgcggct acggctggcg ataaagcgcg
     4201 caacaaatcg gctgtcatcg agatagccat gttcatggca ccaggcaata acgcgctcgt
     4261 aatcttctgc cgtagcatca atctcttctg ggccattttt gcccataatc ggtgccgcga
     4321 gtttacgtcg cagttcttgc tcactgtgat cgcgcaccgc cagaatgcgt accgcacgat
     4381 ccaacaggcg agcatatgcc gggcgacggg acgttgattc tgtcatggca tatccttaca
     4441 acttaaaaaa gcaaaagggc cgcagatgcg acccaggttt tgctgataaa cagatttaat
     4501 agtcaatgag tgtgcagaat gttcccgcag gcccaattcg ttagaggatc gaggtgagag
     4561 taatttttca cgccagtgct ggcctgttcc cctcacccta accctctccc caaaggggcg
     4621 aggggaccgt ccgtgcacac attatctatt atcatcaata acgaacgatt aaaaatcttc
     4681 gttagtttct gctacgcctt cgctgtcatc tacagagaaa tccggcgttg agttcgggtt
     4741 gctcagcagc aactcacgta ctttcttctc gatctctttc gcggtttccg ggttatcttt
     4801 cagccaggca gttgcattcg ctttaccctg accgatcttc tcacctttgt agctgtacca
     4861 cgcgcctgct ttctcgatca gcttctcttt tacgcccagg tcaaccagtt cgccgtagaa
     4921 gttgatacct tcgccgtaga ggatctggaa ttcagcctgt ttaaacggcg cagcgatttt
     4981 gttcttcacc actttcacgc gggtttcgct acccaccacg ttttcgccct ctttcaccgc
     5041 gccgatacga cggatgtcga gacgaacaga ggcgtagaat ttcagcgcgt taccaccggt
     5101 agtggtttcc gggttaccga acattacacc aattttcata cggatctggt tgatgaagat
     5161 cagcagcgtg ttggactgct tcaggttacc cgccagctta cgcatagcct ggctcatcat
     5221 acgtgccgca aggcccatgt gagagtcgcc gatttcgcct tcgatttccg ctttcggcgt
     5281 cagtgccgcc acggagtcaa cgacgataac gtctactgcg ccagaacgcg ccagggcgtc
     5341 acagatttcc agtgcctgct cgccggtgtc cggctgggag cacagcaggt tgtcgatatc
     5401 gacgcccagt ttacgtgcgt agattgggtc cagcgcgtgt tcagcatcga taaacgcaca
     5461 ggttttacct tcacgctgcg ctgcggcgat cacctgcaac gtcagcgtgg ttttaccgga
     5521 agattccggt ccgtagattt cgacgatacg gcccatcggc agaccacctg ccccaagcgc
     5581 gatatccagt gaaagcgaac cggtagagat ggtttccaca tccatggaac ggtcttcacc
     5641 caggcgcatg atggagcctt taccaaattg tttctcaatc tggcccagtg ctgccgccaa
     5701 cgctttctgt ttgttttcgt cgatagccat ttttactcct gtcatgccgg gtaataccgg
     5761 atagtcaata tgttctgttg aagcaattat actgtatgct catacagtat caagtgtttt
     5821 gtagaaattg ttgccacaag gtctgcaatg catacgcagt agcctgacga cgcaccgcat
     5881 cacggtcacc gctgaagcat tcccgccggg taatgccttc accacgggca gtggcaaaag
     5941 caaaccagac ggtgccgaca ggcttctctt cactgccgcc atccggcccg gcgataccac
     6001 taatagacac ggcataatca gcacgagccg ctttcagtgc gcctatcgcc atttccacca
     6061 cgacgggttc actcaccgcg ccatgctgcg ccagcgtctc ttcgcgtacg ccgatcatct
     6121 gcgctttggc ttcgttactg taggtgacaa atccgcgttc aaaccaggca gagctaccgg
     6181 caatatcggt aatcactttc gctacccaac cgccggtaca agactcggca gttgttacgg
     6241 ttgcgccacg ggctttcagc gcctgcccaa cctgttcgct taactgcatc agttcactgt
     6301 cagtcatcac aacctctgtc agtcaaaaat ttatgccgga caagatagca ctttcgtcag
     6361 gaagatggtg ttggcgttgc aattttacga ggaggattca gaaaaaagct gattagccag
     6421 agggaagctc acgccctcct ctggctaata gttactgcac gcgcgccagc gccacggctt
     6481 gtcctaactg ccagaccgcc attgcgtaat gggtgctgtg gttataacgg gtaatggtgt
     6541 agaagttcgg cagaccgtac cagtactggt aaccggtacc gacatccaga cgcagcaggc
     6601 tggcttgttg atggttgccc agcggctgct gtggcgttaa acctgccgcg gcaagctgcg
     6661 aaatgctgta cttagttttg aagccatttg gcaagcctgg agcctgaccg tttgccatta
     6721 ccgcgacctg atcgcctttc acccagccgt gcgctttgaa atagttcgcc acgctaccga
     6781 tcgcatcaac cggatcccac aggttgatat gcccgtcgcc gctgaaatct accgcatatt
     6841 gtttgtaaga cgacggcata aactgtccgt agcccatcgc cccggcaaag gaacctttca
     6901 gattgagcgg atcgtcctgc tcgtcgcgcg ccatcaacag gaaggtttcc agctcgccag
     6961 agaaatactc cgcgcggcgt gggtagttaa atgacagcgt tgccagcgca tcgaggatgc
     7021 gagttttccc catcacgcgc ccccagcggg tttcaacgcc gataatcccg acgataattt
     7081 ccggcggtac gccatacacc tgccacgcgc gattcaacgc atcttcatac tgattccaga
     7141 aaaccacacc gttctgcacg ttgtccggcg taataaattt tttgcgataa cgcagccatg
     7201 cgccgttcgg acctgatggc ggtttcaccg atgtggttgg tgcctggtta tccatcagcc
     7261 gcagtaccga atccagacgt ttcgcctggg agagaatttc ctgcaactgc tgacgatcga
     7321 aaccgtgttt gttcaccatt ttgtcgatga actgctgggc attcgggtta ttagcgaaat
     7381 cgccgcccat ctgcatcaca ttgtgctgcg gctcaagcag gaagccgcca gacggcgttc
     7441 cggtggtcgt atcagtctca gtaggttttg gcttgctgct acaggcggca agcaacacaa
     7501 aaaggggaag caatgttaca taacgacgct tgaacatgag gggtccattt aacagattca
     7561 accaggggca agtatggtaa agcatcacgc cccgcacaag gaagcggtag tcactgcccg
     7621 atacggactt tacataactc aactcattcc cctcgctatc cttttattca aactttcaaa
     7681 ttaaaatatt tatctttcat tttgcgatca aaataacact tttaaatctt tcaatctgat
     7741 tagattaggt tgccgtttgg taataaaaca ataaatcctg aaggagagaa caatgataga
     7801 aaccattact catggtgcag agtggtttat cgggctgttc caaaagggcg gagaggtgtt
     7861 taccgggatg gtgaccggca ttcttccgct gttgattagc ctgctggtta tcatgaacgc
     7921 actgattaat tttatcggtc agcatcgtat tgaacgtttt gctcaacgtt gcgccggtaa
     7981 ccctgtttcc cgttacctac tgttaccgtg cattggcacg tttgtctttt gcaatccgat
     8041 gaccttaagc ctgggtcgct ttatgccgga aaagtacaaa cccagctact acgcggcggc
     8101 ctcttatagc tgccactcaa tgaatggcct cttcccccat atcaaccctg gcgaactgtt
     8161 tgtttatctt ggcattgcca gcggtctgac aacgctgaac ctgccacttg gcccactggc
     8221 ggtgagttat ctgctggttg gtctggtcac caatttcttc cgcggctggg tgaccgatct
     8281 gaccaccgcc atttttgaga aaaagatggg cattcaactt gaacaaaaag ttcacctggc
     8341 aggagcaaca tcatgacgca tattcggatc gaaaaaggaa cgggtggctg gggcggcccg
     8401 cttgagctga aagccacgcc gggaaaaaaa atcgtctata tcaccgccgg tacccggcct
     8461 gcgattgttg acaaactggc acagcttact ggctggcagg ctattgacgg atttaaagaa
     8521 ggtgaacccg cggaggcgga aattggtgtc gcggtaatcg actgtggcgg cacattacgc
     8581 tgcggcatct atccgaaacg acgtattccc accattaata tccactcgac gggcaagtcc
     8641 ggtccgctgg cgcagtacat tgtggaagat atttatgtct ctggcgtaaa agaagaaaac
     8701 atcactgtag tgggtgatgc gacaccacaa ccctcttccg tgggccgtga ctatgacacc
     8761 agtaagaaaa tcaccgaaca aagcgatggt ttactggcga aggtgggaat gggcatgggg
     8821 tccgccgttg cggtgctgtt tcaatctggt cgtgacacca tcgacactgt attaaaaacc
     8881 attctgccgt ttatggcatt cgtctcggcg ctcattggca tcattatggc ttctggcctt
     8941 ggtgactgga ttgcccacgg tcttgctccg ctggcgagcc atccactggg tctggtcatg
     9001 ctggcgctca tctgctcctt cccactgctt tcacctttcc tcggcccagg cgcagttatc
     9061 gcacaggtta tcggcgtatt gattggcgtg cagattggtc tcggcaatat tccgccgcat
     9121 ctggctttac cggcactgtt tgccatcaac gcgcaggcgg cctgcgactt catcccggtc
     9181 ggtttgtcgc tggcggaagc ccgtcaggac acggttcgcg tcggtgtccc ttctgtactg
     9241 gtgagccgct ttttaaccgg cgcaccgact gtactgatcg cctggtttgt ctccggtttt
     9301 atctatcaat agaggctgaa acatgaccgt tatttatcag accaccatca cccgtatcgg
     9361 cgcgagtgcc actgacgccc tcagcgacca gatgctcatc acctttcgtg aaggcgcgcc
     9421 agcggacctc gaagagtatt gcttcattca ttgccacggc gagttgaaag gtgcactcca
     9481 tcccggtttg caatttacgc tcgggcagca tcgctatccg gtgaccgccg ttggcagcgt
     9541 ggcggaagac aaccttcgcg aactgggtca tgtcaccctg cgcttcgatg gtttaaacga
     9601 agcggaattt ccgggcactg tccatgtggc aggccctgta cccgacgata tcgcgccggg
     9661 atcggttttg aagtttgaat ctgttaagga gtaaaaaatg aatcaggttg ccgttgtcat
     9721 cggtggtggg caaacattag gcgcgttcct gtgccacggt ctggctgccg aggggtatcg
     9781 cgtcgcggtt gtcgatattc agagcgacaa agccgcaaat gtggcacaag aaattaacgc
     9841 cgaatatggt gaaagtatgg cgtacggttt tggtgctgac gccactagcg agcaaagcgt
     9901 tctggcgctc tctcgtgggg tagatgaaat ctttggtcgc gtggatttgc tggtctatag
     9961 cgccggaata gcaaaagcag cctttatcag cgacttccag ctcggcgatt ttgaccgttc
    10021 gctacaagtg aatctggtgg gttatttcct gtgtgcccgt gaattttcgc gtttgatgat
    10081 ccgcgacggg attcagggtc gcattattca gatcaactcg aaatccggca aagtgggcag
    10141 caaacacaac tctggctaca gcgcagcgaa atttggtggc gtcgggctga ctcaatcact
    10201 ggcgctggat ctggcggagt acggcattac ggtgcattca ctgatgctcg gtaacctgct
    10261 gaaatcgccg atgttccagt cactgttgcc acaatacgcg accaagctgg gtatcaaacc
    10321 ggatcaagtc gagcagtatt acatcgacaa agtaccgctc aaacgcggct gcgattatca
    10381 ggacgtgctg aatatgctgc tgttctacgc cagtcctaag gcgtcgtact gcaccggaca
    10441 gtcgatcaat gtcaccggcg gtcaggtgat gttctgatta acagcggaga tccgttaagg
    10501 atctccgtga gactatagaa tgcctgatgc gctacgctca tcaggcatac aggacttccg
    10561 ccactacatt aaggaaaagt tatggtatcc gcactcatca ccgtcgccgt tatcgcctgg
    10621 tgtgcgcaac tggccttagg cggctggcaa atttctcgtt ttaaccgtgc cttcgacaca
    10681 ctatgccagc aagggcgggt tggcgtgggc cgttccagcg ggcgctttaa accgcgggtc
    10741 gtggtcgcca tcgcgctgga cgatcagcag cgcatcgtcg acaccttgtt tatgaaagga
    10801 ctgaccgtct tcgcccgacc gcaaaaaatt cccgcaatta ccggtatgca tgcgggtgat
    10861 ttacagcccg atgtgatctt tccccatgat ccactatcac agaatgctct atcattggcg
    10921 cttaaactga aacgtggata atttcgttgt gaatgttact tgcttgcgaa gttatcattt
    10981 tgaaacctaa atcaggtaat cacgcccatg aaacctcgtc agcgtcaggc cgccattctg
    11041 gagtatctgc aaaagcaggg taaatgctcg gttgaagaat tggcgcaata ctttgacacc
    11101 acaggcacaa ccattcgcaa agatctggtc attctggaac atgccggaac cgtcattcgt
    11161 acttatggcg gagtggtgtt gaataaagag gaatccgatc cgcctatcga tcataaaacg
    11221 ctcatcaaca cccacaagaa agagctgatt gcagaagctg ccgttagttt tatccatgat
    11281 ggcgattcga tcattcttga tgctggcagt accgttttgc agatggttcc cctgctctcg
    11341 cgctttaata acatcacggt gatgaccaac agcctgcata tcgtcaatgc gctatccgaa
    11401 ctggataacg aacaaactat cctgatgcca ggcggaacgt ttcgtaaaaa atcggcctca
    11461 tttcacggac agctggcaga gaatgccttc gaacatttca gtttcgataa attgtttatg
    11521 ggcaccgacg gcatcgatct caatgcgggc gtaaccacct tcaacgaggt ttataccgtc
    11581 agtaaggcaa tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc atcaaagttt
    11641 ggccgtaaaa gccccaacgt agtttgcagt cttgaaagcg tcgataagct gattaccgac
    11701 gcaggtatcg atccggcgtt tcgtcaggcg ctggaagaga aagggatcga tgtgatcata
    11761 accggagaga gcaatgagtg aagcactact gaacgcggga cgtcagacgt taatgctgga
    11821 gttgcaggaa gcaagccgtt taccggaacg tctgggcgat gattttgttc gcgccgccaa
    11881 tatcatcctg cactgcgaag gcaaagtggt ggtttcggga attggcaaat cgggccacat
    11941 tggtaagaaa atcgccgcaa cacttgccag taccggcaca ccggcttttt ttgtccatcc
    12001 ggcagaagcg ctgcacggcg atctggggat gattgaaagc cgcgatgtga tgctgtttat
    12061 ctcttactcc ggtggcgcga aggaactgga tctgattatt ccgcgtctgg aagataaatc
    12121 tatcgcgctg ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg cggcgaaagc
    12181 ggtgctggat atctccgtag aacgcgaagc ctgcccgatg caccttgcgc cgacctccag
    12241 caccgtcaat accctgatga tgggtgacgc gctggcgatg gcggtcatgc aggcgcgcgg
    12301 atttaatgaa gaagattttg cccgctccca cccagccggg gcactgggcg ctcgcttgct
    12361 gaataaagtg catcatctga tgcgccgtga cgatgccatc ccacaggtgg cgttaaccgc
    12421 cagcgtgatg gatgcgatgc tggaactcag ccgcaccggt ctggggctgg tggcggtatg
    12481 tgacgctcaa caacaggtac aaggcgtctt taccgacggc gatttacgtc gctggctggt
    12541 tggcggcggc gcactcacca cgccagttaa tgaagcgatg acggtcggcg gcaccacgtt
    12601 gcaatcgcaa agtcgcgcca tcgacgccaa agagatcctg atgaagcgca aaatcactgc
    12661 cgcaccggtg gtagatgaaa acggcaaact caccggcgca attaacctgc aggatttcta
    12721 tcaggccggg attatttaat ccttcattcc cagacgtttc gccagccgat gcaggttggc
    12781 gacgtcggtt tccagcatcc gcgcgcaggc agcccagttg tgatgatttt gtgccagcgc
    12841 ctgacgaata gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt gcttaacaac
    12901 gggcaccgcc gccacttctg gcgtcggcaa cgtcacctca ggaaaagcaa aatgttgcgc
    12961 ctcaagaatc acttcatcgc cgttgcgggt ggctctcgac agcactaccg cccgatgaat
    13021 agcatgttcc agttcgcgca cgttccccgg aaaacgatag tgttgcagta aatttcgcgc
    13081 tccggcactt aataccacgc gggagagccc cagtcgcaaa cgacactgct cgcagaaata
    13141 ccccgccagc agaatgacat catcgccccg ctcacgcagc ggcggcaccg aaagtggaaa
    13201 cacgctcagg cgatgaaaca agtcagcgcg aaatcgccct gccagcacct cttcgcgtaa
    13261 gtcgcggtta gtcgccgcca gcacgcgcac atcgacccgc aaactgcggt catcgccaac
    13321 gcgctgaata tcgccatact gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa
    13381 ctcgccgatc tcatccagaa acagagtgcc gttatccgcc atttcaaact tcccgctgcg
    13441 gttactgata gcgccagtaa acgctccttt cacatgcccg aacagttcac tttccgccac
    13501 actttccggc agtgcggcac agttgagata gaccagcgga ttcaccgccc gtggcgaggc
    13561 ttcatgaatc gctttcgcca ccagctcctt accggttccc gtctcaccgc tgatcaggac
    13621 gttgagatcg gacgccgcca caatctcaat ctcttttttc agttgcgtca tgccagggga
    13681 caagccaatc atctgcgtct gtttcaccgc ttcaaacggc gtggcatcgc ctggcatcat
    13741 attctggctt tccagttgtt caatcagcaa cgcattgctt aacgctcccg ccgccagcgc
    13801 agcaatcagc cgtagctctt cgtcgctgaa aacatcgaac tgatcgggct gcatcccgtc
    13861 gagcgtcagt gcgccgatca ggttttgccc ggcaaacaat ggcagaccaa cgcaggcgtg
    13921 aaccttcaga ctctcctgcc caggaatcaa accgtcatag ggatcgggca attcgctgtc
    13981 tgcgggaaag cgcaccacat ccccggcgcg ggcaatcgct tccagccgtg gatgcccttc
    14041 cagcgcaaag cgtctaccga gtacatcctt tgccagaccg tcgatggcaa gcggaataaa
    14101 ctgccgcgaa tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct gacgtagcgt
    14161 ggtgatcagg cgctgaaaac gatcctgatg accaatccca cgctgcaatt cgatggcgat
    14221 attcgccagc acatcaacgg aaaaactcat ctttgcctca ctgtcaattt gactatagat
    14281 attgtcatat cgaccatttg attgatagtc attttgacta ctcattaatg agcatacttt
    14341 tatttatata gtaaaaacaa tcagataaaa aactggcacg caatctgcaa ttagcaagac
    14401 atctttttag aacacgctga ataaattgag gttgctatgt ctattgtggt gaaaaataac
    14461 attcattggg ttggtcaacg tgactgggaa gtgcgtgatt ttcacggcac ggaatataaa
    14521 acgctgcgcg gcagcagtta caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc
    14581 gacaccgtcg accataaatt cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat
    14641 ctggcggata tcgattacat cgtgattaac catgcagaag aggaccacgc tggggcgctg
    14701 accgaactga tggcgcaaat tcccgatacg ccgatctact gtactgccaa cgctatcgac
    14761 tcgataaatg gtcatcacca tcatccggag tggaatttta atgtggtgaa aactggcgac
    14821 acgctggata tcggcaacgg caaacagctc atttttgtcg aaacaccaat gctgcactgg
    14881 ccggacagca tgatgactta cctgacaggc gacgcggtgc tgttcagtaa cgatgctttc
    14941 ggtcagcact actgcgacga gcatctgttc aacgatgaag tggatcagac ggagcttttc
    15001 gagcagtgcc agcgttacta cgccaatatc ctgacgccgt tcagccgcct ggtaacgccg
    15061 aaaattaccg agatcctggg ctttaactta ccagtcgata tgatagcgac ctcccacggc
    15121 gtggtctggc gcgataaccc gacgcaaatt gtcgagctgt acctgaagtg ggccgctgat
    15181 tatcaggaag acagaatcac cattttctac gacaccatgt cgaataacac ccgcatgatg
    15241 gctgacgcta tcgcccaggg aattgcggaa accgacccac gcgtggcggt gaaaattttc
    15301 aacgtcgccc gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg
    15361 ctggtcggca cttcgacgat gaataacgtg atgatgccga aaatcgccgg gctggtggag
    15421 gagatgactg gtttacgctt ccgtaacaaa cgcgccagtg ctttcggctc tcacggctgg
    15481 agcggcggtg cggtggatcg tctttccacg cgcctgcagg atgcgggttt cgaaatgtcg
    15541 cttagcctga aagccaaatg gcgaccagac caggacgctc tggagttatg tcgtgaacac
    15601 ggtcgcgaaa tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg
    15661 gtggttaaag aagaaacctc tgccaccacg acggctgacc tcggcccacg gatgcagtgc
    15721 agcgtctgcc agtggattta cgatccggca aaaggcgagc caatgcagga cgttgcgcca
    15781 ggaacgccgt ggagtgaagt cccggataac ttcctctgcc cggaatgctc cctcggcaaa
    15841 gacgtctttg aagaactggc atcggaggca aaatgagtaa cggcattgtg atcatcggtt
    15901 cgggcttcgc cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc actattccat
    15961 taaccctgat tgccgccgac agcatggatg agtacaacaa acctgacctc agccatgtta
    16021 tcagtcaggg gcaacgtgcc gatgacctta cccgccagac ggcgggtgaa tttgccgagc
    16081 agtttaatct gcacctgttt ccacaaacct gggtgacgga tatcgatgcc gaagcccgtg
    16141 tggtgaaaag ccagaataat cagtggcaat acgacaagct agtactggca accggtgcca
    16201 gtgcctttgt cccgcctgtg cctgggcgtg agttaatgct gacgttaaat agtcagcaag
    16261 agtatcgcgc ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt gttggcggtg
    16321 gtttgattgg tagcgaactg gcgatggatt tttgtcgtgc aggcaaagcg gtcacgctaa
    16381 tcgacaacgc tgccagtatt ctggcgtcat taatgccacc ggaagtaagc agccgcttgc
    16441 agcatcggtt gacggagatg ggcgttcatc tgttgttgaa atctcagttg caggggctgg
    16501 aaaaaacaga ttctggcatt ctggcaacgc tggaccgcca gcgcagtatc gaagtggatg
    16561 cggtaattgc cgccaccgga ctgcgcccgg aaaccgccct ggcacgacgc gccgggctga
    16621 cgattaatcg cggcgtttgc gtcgatagtt atctgcaaac cagtaatgcc gatatttacg
    16681 cgctgggcga ttgcgcggaa attaacggtc aggtattgcc gttcctccag ccgattcaac
    16741 ttagcgcgat ggtgctggca aaaaatcttc tcggcaataa cacgccactg aaactaccgg
    16801 cgatgctggt gaaaatcaaa acgccggaat taccgctgca tctggcaggc gaaacccagc
    16861 gtcaggattt acgctggcaa attaataccg aacgccaggg aatggtggcg cgcggcgttg
    16921 acgatgctga ccagcttcgc gcctttgtgg tcagtgagga tcggatgaaa gaggcatttg
    16981 gattgttgaa aacattgccg atgtaggtgg gctactgtgc ctaaaatgtc ggatgcgacg
    17041 ctggcgcgtc ttatccaacc tacggggagc gcatgtgttg gtcacatact gcgttcatgt
    17101 cgcatccacc tactgtgccc aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta
    17161 cggggacgca tgtgtaggcc ggataaggcg tttacgccgc atccggcaat ggtgtccaaa
    17221 tgcaacacgt tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc
    17281 ccctgcccca gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg
    17341 aaatcagcga gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg
    17401 ctaaatacca gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa
    17461 ccctgcgcca gcgcatcatg aaacgcccac gcgcgttgat taaccggtgc ctgccagttc
    17521 agccactgct gccagaaagt ggcgagatcc agttgattgt ccaccagcgg cattgtcacc
    17581 ggatgcgtca ctccgtggca cgaagctgcg agtgcctcca gagcacaagc cgcttcacct
    17641 tcataactta acgtggctgg cgcacagccc agtgccgccg ccacggcatc gaacaaccgc
    17701 ccacacgatg acgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagcacgctc
    17761 cagttttgct gttgcacact tgctgtttca gaataattct gccactccgg cacaaagcgc
    17821 aggcactgcg ccagcaggtt tcgccacggc tgcttcgccg ccaaatcgcc acccggaagc
    17881 gccactgcag gcaagccgcc caggtgctca cattcgcgat agttcacccg cagacactcg
    17941 ccgccccaca aagcgccgtt ctcccccata ccgataccat cgagcgtcaa agcaatgaca
    18001 tcaccgccat ccagcggcca ctgatgctct gccagacacg ccgctgcgtg ggcatgatga
    18061 tgcagcaccg tttgcgtcgg cagattcatt tcgcgcgccc actggctgga gacatagccc
    18121 ggatgtgcgt catgcacaac gtattgcggg gtgaaatcgt agatgttttg catcaggcgt
    18181 aacgcttcgc gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc
    18241 aacactgctt gttcaccgcg caccaggcag aaggtgtttt tcagatctgc gccgagacac
    18301 agcacaggcg gcacattttt aaagcccgga ggcaaagcca gtgcgtccgg tacatacccc
    18361 cgcgaacggc gcagcatttc gccgctttcg cgcaccaccg aatcatccat ccgctgcacg
    18421 atgtcgcggt tatgtatcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt
    18481 tcgttgctga tagccggtgg tttaccgctc aggttgccgg aggtcatcac cagcgggcat
    18541 tgcagttcct gtaacagcaa atgctggagc gggttcgcag gcaacatcac gccgacttcg
    18601 ttaaggccag gggcgatatc atcacaaagc tcaggaacgt attttttatc caccagcaca
    18661 atcggcgcgg cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaacc ttctgccact
    18721 ggcaagatga ccgccagcgg tttcgccggg cgatgtttgc gcgcccgaag tgtcgccacc
    18781 gcggtactgt tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca
    18841 atgttgccca tttttaactg tgcgatagcc gcctgtaatg ccgcctcttg ttccgcatgt
    18901 tcaccatgac ttacccattc aagatgtggg ccacactccg ggcaggccac cggctgggcg
    18961 tggaagcgac gatcgagcgg gtcgcggtac tctttatcac aggctggaca cagcggaaac
    19021 tccgccatta cggtaaacgg gcggtcgtaa ggcatggcgc gaataatggt gaaacgcggg
    19081 ccgcagtggg tacagttgat aaacggataa cgataacgcc gttcgcctgg ggtattcatt
    19141 tcggcaaggc aagcagggca agtagcggca tccgggacaa tttgcgtatt catggcacct
    19201 cccgcgctct ggcggatagt gaactcggtg ggcagttgtg accagataaa cggctcacgc
    19261 tcgacgctat caatacgcgc cagtggcgga cagtgctgat gcaattgaac aagaaacgtt
    19321 tccgggtctt ccagcagccg gacttctacg ccatcgccgt cattacagac atcgccgtga
    19381 agatttaatt gctgtgccag ctgccagaca aacggacgaa aaccgacgcc ctgcactttg
    19441 ccacgaatac gcagttggac accgcaagat gtgttttttg ccattgagtt attcccgcca
    19501 tcatgaattg cgtaacccgt ccagccggac acgacagcgt cgcatccggc agtcacaggt
    19561 cggcgatacc gcccgcttcg tattctacga atatttccgg gaattccttt gatgccagaa
    19621 cagttctgta agatttttag aacatcagcg ccgtacggcg gcgtttttct gcgctcagtt
    19681 gttcaagttt attacgatcg acacaaatta gcgcatgggt cgggcaagcc gccatacacg
    19741 ccgggccgtc ttcacgatgg ttgcacaggt cgcatttatt ggcttcagct ttgtcagccc
    19801 gtacattcag ccccgcgccg ctgttgcgga tcaccggacg taccaccact tccatcgcgc
    19861 catacgggca agccaccacg caggttttgc aaccaatgca acgttcctgc atcacatgaa
    19921 caaacccttt atcacggctg atagcaccat tcgggcagac gttagcgcac ggtgcatctt
    19981 cacactgacg gcagactgtc gccgtggaaa tgttcacacc tttaatgaca tggattcgcg
    20041 gtaaaaaggt ttccggggtc agcgatgcac agtcctgatt ttcctgatga gaaaccacgc
    20101 acgccacttc acaggtacgg caaccaatac atttactcgc gtcagcaatg atgaaacggt
    20161 tcatcaaatt ctccagcaat gacagttaat gcgccgatac attcacaaat catgccagtt
    20221 tttaatttgc tgttatttaa ggaaattaat ttctgtaatg caggaaaaaa gatgtcatcg
    20281 acactagtga cgatgacatg tgatgacaat gtttatcgcg aaagggcaat gagtgagtcg
    20341 cggcggatca gttttccgct gaaggttttc ggcggtgaga aatccccgcc atcgagcata
    20401 aaaatcagcc gtccaataat ttcctgaatc atctctgtta ccggaatttt tacgctggag
    20461 agcgccggaa cggtgtaggg ggcaattgcg atatcatcga atccgataac tgacacctgc
    20521 tctggcaccg ctacgccgcg ctcgtgcaac gctttcatcg cacctatcgc catatcgtcg
    20581 ttactggcaa ctaacgcgct aaatttagcc ccacgttcga gcaacgtttc taccccttcg
    20641 gccccgctgg cgggcgtcca tttaccgtta gcgataagtt tttcattgag cgcaatacca
    20701 tgctgcgcca gcgcgtcttt atacccagca agacgttcaa tgctggtggg ggaatccatc
    20761 gagccggtaa ggaaagcaat ctcctgatgt ccggcgttta tcaactctgc cacggcatta
    20821 aagctggtct gtttatgatc gcaccagacg ctatggctgc tgtttttgcg caggcggcga
    20881 ttaagcacca ttatcggctg actgtgcgcg tcaatgatgt catcaatctc atccacgctt
    20941 aaaaaacgcg ggtaaatcat aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc
    21001 tggcgctctt cttcggcgct gtgtttacca tctgccaata gcaactgccg ccctttctct
    21061 tccgccattc gcgcggcatg aaagagtaat tcactaaaat aaatgccgtg gtaaagcgtg
    21121 ttggtcacta ccagccccag cgtctgagta ctcttcgccg acagattgcg cgccagcaag
    21181 tttgggcggt aaccgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg
    21241 acgtagccat tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc
    21301 gccacttcca gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac
    21361 tgcaccgttt gccgattgtc cttgcgcaat cggcgggaaa aatattcagg tgaccggttt
    21421 cacaaatata aaaaatgaac aattaactct cttgcttatt tagtgacaac tattcatgat
    21481 tttgtgaaac cggtttccta attccgtttc agcatcggca tttttccgtt acgtcgactg
    21541 ataacaacta catctaccct actgataaca ggataaaatc cgatggccaa aaattatgcg
    21601 gcgctggcac gctcggtgat agcggcactg ggcggcgttg ataacatctc ggcagtcacg
    21661 cactgtatga cgcgtttgcg ctttgttatc aaagatgacc agcttatcga cagcccgacg
    21721 ttaaaaacca tccccggcgt gctcggcgtg gtacgtagtg acaaccagtg tcaggtgatt
    21781 atcggcaata ccgtttcaca agcctttcag gaagtcgtca gcctgctgcc gggagatatg
    21841 cagcccgcac agcccgtggg taaacccaaa ctcacgctgc gtcgcattgg tgcggggatc
    21901 cttgatgcgc tgatcggcac catgtcaccg ctgatcccgg cgattatcgg cggatcgatg
    21961 gtcaaactgc tggcgatgat cctcgagatg agcggcgtgc tgacaaaagg atcgccgacc
    22021 ttaaccattc tgaatgtgat tggtgacggt gctttcttct tcctgccgct aatggtcgcg
    22081 gcatctgccg ccatcaaatt taaaaccaat atgtcgctgg cgattgccat cgcaggtgtg
    22141 ctggtacatc cgagctttat tgaactgatg gcgaaagcgg cccagggtga acatgtcgaa
    22201 tttgccctga ttccggtcac cgcggtgaaa tatacctaca cggtgatccc ggcgctggtg
    22261 atgacctggt gcctgtcata tatcgaacgc tgggtggatc gcattacacc ggctgtgacc
    22321 aaaaacttcc tcaagccaat gctgattgtg ttgattgccg caccgctggc aatcctgctg
    22381 attggcccga ttggtatctg gatcggtagc gccatttcgg cgctggttta caccattcat
    22441 ggttatctgg gctggctttc agtcgccatt atgggcgcgc tgtggcctct gctggtaatg
    22501 accgggatgc accgcgtctt tacgccaacc atcattcaga ccattgccga aaccggcaaa
    22561 gaagggatgg tcatgccgtc agagatcggc gctaacctgt cgctgggcgg ttcatcactg
    22621 gcggtggcgt ggaaaacgaa aaacccggaa ctgcgccaga cggcgctggc agcggcggca
    22681 tcagccatta tggcggggat ttccgaaccg gcgt
//
LOCUS       DAEVZA010000079        22650 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEVZA010000079 DAEVZA010000000
VERSION     DAEVZA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.079"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            53..730
                     /locus_tag="LAF92_003034"
     CDS             53..730
                     /locus_tag="LAF92_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBI9792001.1"
                     /translation="MSIIFNGHYRMKHRTWITDALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPASAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EMKGSSH"
     gene            730..1077
                     /gene="tnpB"
                     /locus_tag="LAF92_003035"
     CDS             730..1077
                     /gene="tnpB"
                     /locus_tag="LAF92_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI9792002.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGICI"
     gene            1097..2668
                     /locus_tag="LAF92_003036"
     CDS             1097..2668
                     /locus_tag="LAF92_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBI9792003.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKT
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
     gene            complement(3262..3576)
                     /locus_tag="LAF92_003037"
     CDS             complement(3262..3576)
                     /locus_tag="LAF92_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792004.1"
                     /translation="MITVIISEVDGWREWKHRARTKDAQTAIIRAMNKHFPRSYNFIP
                     DDIDNAPVLFAAVTRTPNVKITGHIWKPMWNRGICWNVKGPPVIITLIQGAAWNSENK
                     PR"
     gene            complement(3634..4299)
                     /locus_tag="LAF92_003038"
     CDS             complement(3634..4299)
                     /locus_tag="LAF92_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792005.1"
                     /translation="MGSVTKKPSPVVIIKKKRRILSHQDDAVQAVPDDIKPAPVMEFK
                     TDSLPPEIPATVAGSPEQQKKTGRKKKKKKKNNRWNHEFIRHSLDCVKKTFPQLRAEE
                     GGYHAFKIGISKDVMAFISASPECGLTVKEWNCAVRMITGGYKYLQRISVAGTPRYGL
                     DGKVHGTVTEEEALYAQAKLEKHTQRYNARMKETEDARCNQPSKTVTPDSNLKDACIH
                     NGA"
     gene            complement(4402..4992)
                     /locus_tag="LAF92_003039"
     CDS             complement(4402..4992)
                     /locus_tag="LAF92_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HBI9792006.1"
                     /translation="MMKNPLAYFHIATFLLSGKSRNLSEYWKAEVIMRDKFVLHRLIR
                     NGLQRQRGFLMWWRLANEMFISGNQKQRKCAIKIKNALMERYGCDIGLGARIGKGLML
                     PHHAGIVIHGNVDIGENVIIRQNTTIGEKVSDSRENYIVIGDNVDIGAHTCIIGLNVK
                     IGSNVKIGAMSFIMEDVPDNCTYVTRKESRVIIREQ"
     gene            complement(5096..5383)
                     /locus_tag="LAF92_003040"
     CDS             complement(5096..5383)
                     /locus_tag="LAF92_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBI9792007.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKYSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
     gene            complement(5380..5631)
                     /locus_tag="LAF92_003041"
     CDS             complement(5380..5631)
                     /locus_tag="LAF92_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBI9792008.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            complement(6591..7622)
                     /locus_tag="LAF92_003042"
     CDS             complement(6591..7622)
                     /locus_tag="LAF92_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI9792009.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRMAIRELRQLE
                     LTLAAPPPH"
     gene            8594..8857
                     /locus_tag="LAF92_003043"
     CDS             8594..8857
                     /locus_tag="LAF92_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBI9792010.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            8775..9062
                     /locus_tag="LAF92_003044"
     CDS             8775..9062
                     /locus_tag="LAF92_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HBI9792011.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            9504..10037
                     /locus_tag="LAF92_003045"
     CDS             9504..10037
                     /locus_tag="LAF92_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HBI9792012.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            10291..10974
                     /locus_tag="LAF92_003046"
     CDS             10291..10974
                     /locus_tag="LAF92_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HBI9792013.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFSSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            11146..11400
                     /locus_tag="LAF92_003047"
     CDS             11146..11400
                     /locus_tag="LAF92_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HBI9792014.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            12152..13219
                     /gene="pilL"
                     /locus_tag="LAF92_003048"
     CDS             12152..13219
                     /gene="pilL"
                     /locus_tag="LAF92_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HBI9792015.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGGNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSATAPAQDTATTISPAAMVTTSTSPESSTTANSQN
                     KPRNPFRGNSVSPSTPAAIPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            13219..13656
                     /gene="pilM"
                     /locus_tag="LAF92_003049"
     CDS             13219..13656
                     /gene="pilM"
                     /locus_tag="LAF92_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HBI9792016.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQQMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            13670..15352
                     /locus_tag="LAF92_003050"
     CDS             13670..15352
                     /locus_tag="LAF92_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HBI9792017.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            15345..16640
                     /gene="pilO2"
                     /locus_tag="LAF92_003051"
     CDS             15345..16640
                     /gene="pilO2"
                     /locus_tag="LAF92_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HBI9792018.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWTPVVKKQRVPSHQKHK
                     SHVKPRRLTSLKVTVGSRNSRGEISRLTGTGILTRPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLAMNEEPPEKWQVVSSLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVGPTA
                     EEIAARARLQFKKPEPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIQEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            16627..17079
                     /gene="pilP"
                     /locus_tag="LAF92_003052"
     CDS             16627..17079
                     /gene="pilP"
                     /locus_tag="LAF92_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HBI9792019.1"
                     /translation="MRPGKSLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVNLSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            17090..18643
                     /gene="tadA"
                     /locus_tag="LAF92_003053"
     CDS             17090..18643
                     /gene="tadA"
                     /locus_tag="LAF92_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBI9792020.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDITPKVVSLSELQEAGQDDENKGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     VMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEEAVKLAWSRAISSAMRLDPDAIMEGEMRDLVSMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRVPELSED
                     DRAYLERHCNKDSLCSTDNIWFRNPHGCSDCNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMHGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDA"
     gene            18656..19741
                     /locus_tag="LAF92_003054"
     CDS             18656..19741
                     /locus_tag="LAF92_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBI9792021.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLGLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKKQLHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPVLSKI
                     SSPDSWSGALGFLYGLSLFVDNYGAICAVLFAVVTGVISWSLKNWKSRSLADNIMPWS
                     IYKDIQGAAFLLNMAALLKAKMTTLNSLNVLQEFASPWLSTRLDSIIYRVRQGDHLGL
                     ALRQCGYHFPSRESANFLSLLQGDGATELIGNYGQRWLVQTLERVKKRAAVVRLIMLI
                     FLVMSLLLLVMAVVDIQSIGDNSMGSI"
     gene            19758..20372
                     /locus_tag="LAF92_003055"
     CDS             19758..20372
                     /locus_tag="LAF92_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HBI9792022.1"
                     /translation="MLIQNINANVLKKNEKETSHDKGWGIMEQGAIALVVIIVLALVF
                     GGLYMLRSRTGVANESSNIQTIITSTQGLLKGSDGYTFTSGAKMTGALIQMGGVPKTM
                     TVRGTPSSGTATLYNSWGGAVTVAPASTSGFNNGFTVTYEKVPQDACIQIATQISRTG
                     LTNGITLNSTAHSDGKVTTEEASAQCTADNGSTGTNKLIFTING"
     gene            20382..20942
                     /locus_tag="LAF92_003056"
     CDS             20382..20942
                     /locus_tag="LAF92_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBI9792023.1"
                     /translation="MSGGKVPLRLISLILLCSLIYPARASYIAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLKPGAININKDRKTGKASSTDYGLMQINSTHIPKLIK
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKVWRIYRDMKGICLPGQGGRQCRQS"
     gene            20927..21583
                     /locus_tag="LAF92_003057"
     CDS             20927..21583
                     /locus_tag="LAF92_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBI9792024.1"
                     /translation="MQTIVGISLSPVYILPLMFIFSIIGRTLLFRVRYFLSDTEHLWY
                     KTHTAVLSGIWLYSIAVALIILSSSPLIYRIHAVLILSFMLQMAVTDALTGLLPGTFT
                     RRFLIAGILSQITTDIWWLRATEIVSAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWSVLLGAGGFVLWHSTWCIKGHKEGPLGPWLCLGHVLLLLDNLYQPLWVI
                     "
     gene            21571..>22650
                     /gene="pilV"
                     /locus_tag="LAF92_003058"
     CDS             21571..>22650
                     /gene="pilV"
                     /locus_tag="LAF92_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HBI9792025.1"
                     /translation="MGNLMKKYDRGWASLEIGAALLIVMIIVAWGAGIWQDYLKTKGW
                     QAEARLVSNWASAARSYIGKNYTTLQASSTTTTPAVITTTMLKNTGFLSSGFTETNSE
                     GQRLQAYVVRNTQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATG
                     ALRSWSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAI
                     DMGSNNLNNIGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGW
                     LITRNSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQ
                     LERTAVAGASCSPNGLVGRDNTGAIL"
BASE COUNT         6014 a         5539 c         5270 g         5827 t
ORIGIN      
        1 taaccggctc atttaaaccg tctggtctgt ttcctccggc tctacaaaaa taatgtccat
       61 catttttaat ggacactatc gtatgaaaca ccggacctgg atcactgacg ctttacgtct
      121 tcactttgaa gaacatttac cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc
      181 aacagtttgt agtatgttcg tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc
      241 aggcatgtcg gagcaggaac ttgatgcctg cctttacgga caattttcca cggtaccagt
      301 cgtacgtcct gaaagcaccg ttatatccga ggcccccgtg gtaaaaaaac gtccccggcg
      361 gcccaacttc ccttatgagt ttaaaatcgc cttagtggag cagtcactgc agcccggagc
      421 ctgtgtggcg cagatcgccc gggaaaacgg aatcaacgat aacctgctct tcaactggcg
      481 ccatcaatac cggaaaggtg gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc
      541 cgtgacgtta acgccggagc cggataataa aatcccggcc tccgcacagg aaccagagca
      601 gataaataca ccgtccgaca gtctgtgttg tgagctggtt ctgccggccg gaactctcag
      661 gcttaaaggt aaactgacgc cggcgttatt acagacactt atccgcgaaa tgaaagggag
      721 cagccactga tgatatctct ccctgccggt tcgcgtatct ggctggttgc cggtatcacc
      781 gacatgcgaa atggttttaa cggcctggca tcaaaagttc agaacgtcct gaaggatgac
      841 ccgttctccg gacacctgtt catcttccgc ggacgccggg gtgaccagat aaaagtgttg
      901 tgggctgaca gtgacggact gtgcctcttc accaaacgcc tggagcgggg ccgcttcgtc
      961 tggccagtca cccgtgacgg caaggtgcac cttactccgg ctcagttatc catgcttctt
     1021 gaaggtatca actggaagca cccgaaacga acggaacgcg ctggaatctg catataaccc
     1081 gttgtaaagt gaggatatgg acacctcact tgctcatgag aacgcccgcc tgcgggcact
     1141 gttgcagacg caacaggaca ccatacgcca gatggctgaa tacaaccgcc tgctctcaca
     1201 gcgggtggcg gcttatgctt ccgaaatcaa ccggctgaag gcgctggttg cgaaactgca
     1261 acgtatgcag ttcggtaaaa gctcagaaaa acttcgtgca aaaaccgaac ggcagataca
     1321 ggaagcacag gagcgaatca gcgcacttca ggaagaaatg gcggaaacgc tgggtgagca
     1381 atatgacccg gtactgccat ccgccctgcg ccagtcttca gcccgtaaac cgttaccggc
     1441 ctcacttccc cgtgaaaccc gggttatccg gccggaagag gaatgctgtc ctgcctgtgg
     1501 tggtgaactc agttctctgg gatgtgatgt gtcagagcaa ctggagctta tcagcagcgc
     1561 ctttaaggtt atcgaaacac aacgtccgaa actggcctgt tgccggtgcg accatatcgt
     1621 gcaggcacca gtaccttcaa aacccattgc acgcagttat gccggagcgg ggctcctggc
     1681 ccatgtcgtc accggaaaat atgcagacca tctgccgtta taccgccagt cagagatata
     1741 ccgccgtcag ggtgtggagc tgagccgtgc cacactgggg cgctggactg gtgccgttgc
     1801 tgaactactg gagccgctgt atgacgtcct gcgccagtat gtgctgatgc ccggtaaagt
     1861 ccatgctgat gatatccccg tcccggtcca ggagccgggc agcggtaaaa cccggacagc
     1921 ccggctgtgg gtctacgtcc gtgatgaccg taacgccggt tcacagatgc ccccggcggt
     1981 ctggttcgcg tacagtccgg accggaaagg tatccatcca caaaatcacc tggccggtta
     2041 cagcggtgtg cttcaggccg atgcttacgg tggttaccgg gcgttatacg aatccggcag
     2101 aataacggaa gccgcgtgta tggctcatgc ccggagaaaa atccacgatg tgcatgcaag
     2161 agcgcccacc gacatcacca cggaagccct gcagcgtatc ggtgaactgt atgccatcga
     2221 ggcagaggtc cggggctgtt cagcagaaca gcgtctggcg gcaagaaaaa ccagagccgc
     2281 gccactgatg cagtcactgt atgactggat acagcaacag atgaaaacac tgtcgcgtca
     2341 ctcagatacg gcaaaagcgt tcgcatacct gctgaaacag tgggatgcac tgaacgtgta
     2401 ctgcagtaat ggctgggtgg aaatcgacaa caacatcgca gagaacgcct tacggggagt
     2461 ggccgtaggc cggaaaaact ggatgttcgc gggttccgac agcggtggtg aacatgcggc
     2521 ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggagccag aaaagtggct
     2581 gcgctacgtt attgaacata tccaggactg gccggcaaac cgggtacgcg atctgttgcc
     2641 ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag ccgcttacgg
     2701 ccttagaaag cctagtcaaa gagctgtcac gagaacaccg ttagcttagc gtacgatttt
     2761 ttccgaattc tgcggttccc ccaggatgac ctatgttgaa tagaacctaa aacaaaacaa
     2821 atattaatag ggggttctac gccggaaccg ccgaaatttc cgacacagcg aattagtcga
     2881 tttttgaagc atgtttgtcg tgtgctgacg tgtacgttta tggtgccgtg agtgagaatt
     2941 tatccgtacc agccatgtga tacgttgcga acagtgattt ttcactccaa acggctattt
     3001 ttttgctatc cggggtccgc ttggaaaagg gaaattttcc cacgctaaga gaattgagtt
     3061 gaacgagtgg ttacatccag gccagctaac tcactgaacg taaaggctgt acgaaatgtt
     3121 ccggccgtcc gataaccgtt tggcttactt ccgtacattc ccttgcgtag atgtacagaa
     3181 ttgtacgatt tcggacaccg aaacagctgt tgtcccgcca tcctggcgat tcctgaaagt
     3241 catttgtcta aaaaaatagt cttaacgtgg tttattttcc gaattccaag cggccccctg
     3301 gatgagcgtg atgattactg gcggaccttt gacgttccag caaattcccc tgttccacat
     3361 aggtttccag atatgaccgg ttattttcac gttcggtgta cgtgttacag ctgcaaataa
     3421 tacaggtgca ttatcaatat catcagggat aaaattgtaa gatcgtggaa aatgtttatt
     3481 catagcccgg atgatggctg tctgtgcatc ctttgttcgg gcgcggtgtt tccattcacg
     3541 ccagccatcc acctcagaaa taataacagt aatcatcaaa acctcgtgtc gttactcaat
     3601 tctgattcca tagcgggaca gcatttcaca taattatgcc ccgttatgaa tgcaggcgtc
     3661 cttcaaattg ctgtccggtg tgacagtttt cgatggttgg ttgcaacgag catcttctgt
     3721 ctctttcatc ctggcgttat aacgctgcgt gtgtttttca agttttgcct gcgcatacag
     3781 cgcttcttcc tccgttacag ttccatgaac tttgccatcc agaccataac gcggagtacc
     3841 tgcgactgat atacgctgca gatatttgta accgccagta atcatcctta cagcacaatt
     3901 ccattctttt acagttagtc cacactcagg actggccgaa ataaaagcca tgacatcctt
     3961 tgagatgcct attttaaatg catgatatcc cccctcttca gcccgtaact gagggaatgt
     4021 ttttttaaca cagtccagac tgtgtcggat aaactcatga ttccatctat tgttcttctt
     4081 ttttttcttc ttcttacgtc ctgttttttt ctgttgttcc ggggaaccgg ccactgtggc
     4141 tggtatttca ggaggtaatg agtcggtttt aaattccatt actggcgctg gttttatatc
     4201 atcaggaact gcctgtacgg catcatcctg atgagaaaga attcgtcttt ttttcttaat
     4261 aataacaaca ggtgacggtt tctttgtaac actgcccata tacacacctc tgcgtatcgt
     4321 taatatataa tgcacgacat acagatagta tatatctgag tcggcaaagt caccaacaga
     4381 agaatgataa catgtatttc attactgttc gcgaattatc actctggatt ccttcctggt
     4441 gacataagta cagttatcag gtacatcttc cataataaaa gacattgccc cgattttaac
     4501 attacttccg attttcacat ttaacccaat aatacatgta tgggcaccaa tatcgacatt
     4561 atcgccaata acaatatagt tttccctact gtcagaaacc ttttcaccaa tggttgtatt
     4621 ctgccgtata atgacattct caccgatatc aacattacca tgaatgacga ttcctgcatg
     4681 atgaggtaac atgaggcctt taccaatccg cgcgcccagg ccgatatcac atccataccg
     4741 ttccataagt gcatttttta tctttatcgc acatttcctt tgtttttggt ttccagagat
     4801 aaacatctca ttggccaggc gccaccacat cagaaagcct cgttgtctct gcaacccgtt
     4861 tcttatcagg cggtgtaaga caaacttatc ccgcatgatg acttcggctt tccagtattc
     4921 tgacagatta cgacttttac ctgacaataa aaaggtggca atatgaaaat aagctaatgg
     4981 atttttcatc ataaaaacga ccttaaagaa taaagaaaat attctgtatt gtggaataaa
     5041 ataacataac tatcagaaat acttttaaag taatatcagt cagctttaaa gataactatc
     5101 tcattctctc actggcgaga ttataaacgt tactacgttc acgtttacct accgcgacaa
     5161 ccgcaataat tagcatgtca tcaataacct gatagaccag acgaaacccc gatgcgcgaa
     5221 gtttgatttt gtagcagtct tttaatccac gcagttttgc ggaaggaata tgaggatttt
     5281 cgctgtattt ctttagtttt ttcgcaaact gctgttgaat ggatttatcc agtttcagcc
     5341 attctttgag cgcatcatcc ctgaatttta ctgtataagt cataaataac tgtccaggtc
     5401 cacatcatgc agtggctgat tgctgcgttc agtaaccagt ttcaccagtt cctggtcatc
     5461 cagcgcatca agcatccttt catacagttc agcaggcaca cagtagaatg ccggctgatt
     5521 ccggttaaga atggcaacag ggtaaccatc tccggcactg actgtagcca taggattttt
     5581 tttcagttca ctgacacttg cactggtgtc agacagaatg atgttaggca taacgcacct
     5641 ttgagactat ttaacaagtc ttaaagatac ctctgaaagg accggttaac aagtctgtat
     5701 tactcatact tttgcaaata catttaatgc tggttttatt gttctgatgc cgcgtgagcg
     5761 gcatggaagg cggcataatg tagctacata tggcacaaga gtaaaaccgc cgaagcccgg
     5821 cgtaagccgg cactgattga tagatttcac cttacccatc cccaccctgc taagccatac
     5881 ccgctgtcag ccatgagaga gcttctgtgc gcggtcggag tggtcccgac gagggtttac
     5941 ccgaagtcgg ggcgtatctc cgcgttagcg ggccgtcagg gccgcttacg agcgtgtact
     6001 gagaacatcc agccagaaga ctgacagcga tgataatgta gttacaacat tcataattaa
     6061 aagcgatgtt gttcccgccc ttcgggcggg ggcggcctcg cttttcagtt atgaggggtg
     6121 ggcacggtgc tgcgtttgtg tggtgttttt tatttctgat gtctttttgt tgtggtttca
     6181 gaatggtgag gttcggggcc cgcgcgttag cgtgggaggt gcttttaaat tctgagcggc
     6241 gcggccttgg ccgctgcaag cgaaggcctc tttgtatttg ttcacagtag tctcagagat
     6301 aaagactttc cctgcggcga agcaacgcag gggattaaat aatgggttta agactatatt
     6361 gcaggtttgc tgtttcaggt ttgcagcttc ttaaagtttt ttgtttgcgg atttatgtgg
     6421 ataactgaaa caacgtggcc atgttctgat gactgatgcg tggttttatg ggcagatatg
     6481 cccctctgag gggatttaag gtgattactg cggattttgg gctgagcgga accagcgccg
     6541 aaagccccgg aggggctggt gatgattcag tgctgaggga atggtgctgt ctagtgcggt
     6601 ggcggggcag ccagcgtcag ttcaagctga cgcaactccc tgatggccat tcgcttcaga
     6661 tggtcatcag aacaaaaata ggcttcatcc ggaggcagac gcttcctgag atactctgcc
     6721 atctcatgaa tctgctggtc ggcaggcagt ttcttcaggc gtctggcgcg cttactggct
     6781 gctgctttcg cacgacggtg tttcagcgca tcctgactgc gctgcagata ccagcgtttt
     6841 ctggccgcat gtacggagat atcttcatcc tcacgcagaa caccctcccg aatgagctgc
     6901 tggcggattt cactttcacg cagtcgtttc tgctgctgtt cgtgaagttt taccatgtcg
     6961 acgcccagca tctgccagcc tgccggtgtt atccagacgt aacgtggcag acgctggcgg
     7021 gtttcacggt cccacagggt ttcctctgaa acacccagca caccaaaacg tacctgttcg
     7081 gccagcaaac gggaaaggcg ggagaccgtc acttccagtt ccggaataac cttccccttg
     7141 ctgtctttcg ggctgatttc tctggccaga cgggaaacac tcatgcctac ggtatgtgtg
     7201 cccgcatcac tgaagctgac cagaaccggc cagatggcat cgaggaggcg ttgtttttcc
     7261 ggacggaaag catatttgcg accggcatac gttcgtttct gcacgtaaaa aggatgcaga
     7321 gacatgtgca tgcgcaggct ctgctcgccg gtcaccgggt cctttttcac cagacggttg
     7381 taggcgtgcc cgagctgccc gctcagcgcc ttatagcgct caggacgata ccaggacgga
     7441 ttttcgcatt tgggtttggt ggagtgctca ccgcgacggc gcttcgtgcg cttcctttcc
     7501 ggttcaacca ggaaagtggt cgcccttccc gcttcatagt cttcccagaa acggatctgc
     7561 tcttcgggtg ctaactgact ccagtgtact gcgttatatg gagtattctt cagacctgcc
     7621 acaagctgtc gcttatggcg gagcgattgt gccgtgtatt tatacactgc acagggttga
     7681 atctctggta cggtttcatt atactttacc cgtattctgt gaggccccca taatctttct
     7741 ccgtcccgcc aagttcgtcg aaagattgtg ggggttttcc ttttatggct tccggaacat
     7801 cttcggtctt ctgacttact gcccgtaaat gccatctctc ctgaatgcct tatggcgcat
     7861 tctgccgtcc ggctttcgcc gctttgctcc acagagtgcc accctctgcg cagctaataa
     7921 caaccgaaat atacccgctt tacgttaatt tgaaaagcat tacaacgatc tcttctcttc
     7981 cagtgtctcc tcaccgatac ctctaatgag tcaatcttga cctgcgtcaa aaaaataaaa
     8041 tctattttta aaagcagatt tttctgcttt agatccttct tttggattaa aagatcgact
     8101 tttgtcctgc tcacgatcat ttatcttaca accgatccac ctaacagaat aatattctgt
     8161 tttaatgcaa ttaaacgatc taaaaaacct ttaaaaaatc attttgtaga tcattcgatc
     8221 gtaaatagaa taattcttag atgataaaac catttgttac acaacccgcc aattgtgtaa
     8281 caacagcctc tacaagagca tatttgttca tatgttcgtc agaactgcgg attgtctgga
     8341 cgagggtcag acaccgtttc gtgcatatgg accagcatgt tcgaacgcca caaagcagaa
     8401 gcatcacgcc tggatcgctt cggcgcggag cgttaagttt tgacgctttt ttgtatgttt
     8461 tttaagctgt tagttgactc ggtgcgttat actaagtacc ctttactggt cacaggcaaa
     8521 actgtgatcg ggcgtaggaa cccgcgtagt catcgtcggc gcagaagtta atttcttcat
     8581 ttggcgtcgt tttttgccat accataaaga caggaacttg tatttcttgt cgtcatctat
     8641 ggtagctcag gcggggcagg cattagcctg gccggtttcc gatgacgccg gtattcctac
     8701 ccccgtctgg gctatcacca atgagcgtag gaactcagtg atagcgtttt tagttgtcat
     8761 cggagacctt tgctatgtca cctcaactga tacagaaact cccggccatc actctgttgg
     8821 aaggcatgtt tcccgagcta tcaacaaacc agcttaaagt ctgcgttttt tacgcaatgg
     8881 gtgttcctta cgacgccatt gctcagaact gtcggctttc tcccgaaacg gtacgaacct
     8941 acctgaagcg aagcctgaag aacttaaatc tggaaggata tgatgcactt cgctctgctg
     9001 tgctgatgcg aacgttcgtt ttcatgatta gcaatacagc taaagaaaac gaaaaaatgt
     9061 gaacaggtgt ccccattaaa ggggacactt tcatggaaat tgcattttgc actttacagg
     9121 cgatcccaaa ttgataaaca taaacaccaa gcacaagtta ctttaataaa gcaaatcact
     9181 tcagacggtg tcccagttat ttgtatctgg ctcatgcccg atatatggcg accttactct
     9241 gaactgacgt gctgatgttt tatttcgcgt atcacaatac tgtgccactg tattgctgat
     9301 attagtgctt agccgttctt tatctgtgag ttcgccaaat caccgataca gaacgtacta
     9361 gagtctgctg gttatatgcc tgtcagaact gcagtttatc cagatatggg ctggacactg
     9421 cttcagacat atgatttagc atattcgcct gacatcgcaa aatcgtccat catggatcgg
     9481 ttttgcgcgg agcgttgaac ttcgtgaata tagaacacct gaataaccgg aactggtatc
     9541 ttgcgcaata taatacagcc ggcaaaaaca gagaatcttt attctcatgg ctgaatgagc
     9601 aaaacgtcgt accatggact ccattaataa caagaaagat aagacgggca gactccaggt
     9661 gctgttaccg tgaaagaata ttcgctattt ttccagggta cttttttatt ctggctaatt
     9721 ttgatattca accagtatcg gcattacgtc gccatagcgc cttcattgat ttcgtgaagt
     9781 ttggcggtga aataaaaccc gtcaataaag atattgttga tggattaatg aagatttacc
     9841 cggaccctgt gctaaatccg ggagccaggg aagagctgaa tgcggcttcc agcatatggc
     9901 tgacaaaagc acaatatcag tatctcctca gaatggaaaa tactctccag cctgagtcga
     9961 ggatatccct gctcctggaa ctggtatcaa atgccgaaca tcacggcttt atggagcgcc
    10021 ttgtaaacat cccatagcca gtatttcccg gccttcgggc cgggcttttt tcaattcaag
    10081 agaatattcc ggagtgaagc ctgcacgaag ccatccccac catggactcc ccctgtcttt
    10141 tgatggaacc gtgtatttct taatggcgaa agaaataaaa ggcttcaccc cagagtattg
    10201 attcgtttta tcaaaggccg gcccgtgcca tcacgtgtgg cggagagaac ggataccggt
    10261 cttcttttca tctcacagta ggacattcgc atgaccacga cagaacatcc cgtgcgccag
    10321 ttgctctggt gtgcactgaa tgccctgaaa acggcacagg aaaaccacaa tataaccagt
    10381 gaaaccggat tacggcatta cctgcttgaa tggctttctg gtgccagcag acatcctgag
    10441 ttcagtagtc tccctggcga aatcatggcg ttaaaagcat tgaccgaaaa agatcgcaat
    10501 attcccatca tcggaacgct gaatacgctc tttctcagct ctgccactgt cggggaatgt
    10561 ccgttgtttc gttttcgggc tgcgctgggt aaattacaga aagcaggctg gcgcacttat
    10621 gtctgcccat ggcctgagag ggtgttcaat gaatccattg aacgggcctg ttccgggaaa
    10681 cgccatctgc tacaactaag tcgcaccgaa gagtgttttc taccgacagg cgatatgagg
    10741 gcaccagtta cgttgcagct gatcactcca ctacaccgca aaactatgca gcttctggaa
    10801 acggcagagt taatattctc cgacgaagga tttcaggtgg tgagaggata cgaacatcaa
    10861 ttcgggatcg aacgcagaga aaccctcttt cacaccctat ttatcgggct gcccagcttg
    10921 ccagaagatg tctggggtgc aacacaggag cagacgacta tcacattaca ctgaggatgt
    10981 gtaatccccg gcataccccg tttccagtct tcgcagcctg taccgccctt cctgccgttt
    11041 ccgacattct ttctgtcact actcgcttgt gccgtttttt cgcaaccggt acgtgcagtt
    11101 atgacggtac agctgcttct tttcccgttc cggaaggagt ctttgatgcc acagcaacat
    11161 cccggacgac tgcaaatact ggtcgtcgac gcacactgca aacgcaggct gttttcaacg
    11221 aaaacaccga cagaccccga tgagcttgcc cgacgtttct gcacgccgga taactgtctc
    11281 gtggtggtac tacgcgacaa ccgatttctg ttccgactgg agcgtgctcc cggcagccac
    11341 tgccgctggc acaagggcag cagctcccgc catcagcatc tacaggactg gctatcatga
    11401 acgcaaccga agcaaaatgt ttattcaatg gtgtgtgatg atcaggaact gcattttttt
    11461 gttccaaaga attaaacagt taatcctcct tagtctgtta ttttagtcgc ctgcctcagc
    11521 caacatgaaa aataataacg taccattata tctctggttg ctttggtgta cgctttgtct
    11581 gtacatcact accccatggc aatacttacg ttatccatac actgacaact tatcattgcc
    11641 ttatatgtta tatttctgtg tggtggttgt gatttgcatt ttcccactac ttttcagatg
    11701 tgtacttctt attgtaaggt ctgcatctaa atacgacttc acattctttg tgaagaagaa
    11761 aaagggaagc aagaaatcaa ctatacatct tagcccctat gctgacacac gttgcccgaa
    11821 gtcaatgagg aaacactggg atgtattgct ttctctgata tcaaaagcaa ttaataatga
    11881 tgaaatcatt gtcatgcatt cacatctctt tactccagca agaataaata aacttataaa
    11941 gagactgaaa tcttatgatt tgagtttttc agttattaca tacccacgga aaacaagtct
    12001 gtacgaaaaa ttatcaattc cttttatata tctcttattt cagtggtctt tcccgcattt
    12061 gcattcggac ggaatgacag tcgtcattcg tcctctccag ccgcctcaca tcaattaaca
    12121 aactcgtata tacgaacacg gagtctccgt tatgaaaaaa aactgtctga cacttatact
    12181 gccgtcatta ttgtccggct gtgccggaat ttccggcggc aataacgtgg caccatcaga
    12241 tacgcctgct ctggtttttg ttgacggaca aatcagtgag agtgctggcg tcctggcact
    12301 tacgcaacaa cgactttctc cacctaaaat aactccgccc ccgagagtca gcgtgccaga
    12361 gacagtaaaa acagcacagc cagtaagcga atcccgaccg ttaccttcac caacaataag
    12421 tcagaatttc tcccggttaa tcattacagg caagcctggt agcgcccctg taacactatc
    12481 gccgacggcc cgtaaccgga ccgtcagtca gtggataaaa acattactac cggaaggatg
    12541 gaaattcaga caagagaatg cagccacgcc caaactgaat accaaactgg tttcatggtc
    12601 tgccaatgat cagtggaccc gttctctgaa ccggttgctg gaagaacaac acctctgggg
    12661 ccatctggac tgggataata aaacgttgac cgtcacaaca tcggctactg caccggcaca
    12721 ggacacggca accactatct cgccagcagc catggtaacc acatccactt ccccggagtc
    12781 ttcaacgaca gcaaatagcc agaacaaacc acgaaaccca ttcaggggaa acagcgtatc
    12841 tccgtctacg cccgctgcaa tcccgactcc gaccggctcc acagtcaagt ctatcccact
    12901 catgaccggg acacctgtca aaccggtatc tcagggtaaa gagtggcgtg cgccggcagg
    12961 cactacatta agggaaaaca ttattaagtg ggcagaagaa acaaaatgcg aaagcatggc
    13021 atcaacgcac tggatggtca tatggccact ttctgtaacc gattaccgtc ttgatgcgcc
    13081 tttgatgttc cggggatcat ttgaatcagc catggagcag gtatttgagt tatatcggac
    13141 cgcgcaaaaa cctttatacg cacaggcctc ccgcatgcag tgcatcatta cagtttctga
    13201 tacaagggat ggtcgctaat gggatggctg gtcatggccg tcggcctgac cattctgatt
    13261 atcactggaa gctaccagaa tcagcagatg tctgaaacca cgaacgccca gcaatatgca
    13321 agtgcgtctg tatgggcaag tcagatacta atgattgcta acagaatcaa tgatatcaga
    13381 tacgtatcag gccagcagga cggagtaatt tcttcagata aactggctct ccctgtcact
    13441 ccggactccc gaattaaaca tcagttacaa cagggacgtc tgtgggtatg gatgcctgaa
    13501 caaccaggac ttgttgaaac tttgcgttca aaaagtcgtg gttctgcact aatagggatc
    13561 ttccaaaacg ggcaactcac ctggctatca ggaactgcaa ccggtctgac acccccggca
    13621 ggcattactg ctggttctgt cgtctacgtt aactaatctg gatatattca tgaaaaaatc
    13681 acaccagcgt tcaatgaagc tggcggtgct cccctgcatg atcgccgtcg ctctttccat
    13741 ctccggatgt actttcagcg aaatcaataa aatgcagaaa aaagcacagg aggactcagc
    13801 acatgcacgg gaaaaggtat cagccctttc tgcccgcaaa tcacaggctc ttacctggct
    13861 cgataatcaa tggataaacc ctgttccggt cgctcaggta tcaagagaga aaaaacaaac
    13921 agctccggcc tgctacatca cgcaggcaag aaaaggagag atcactctac aggaactggg
    13981 gcaacgtatt actgccgtat gcggcatccc cgtaatcatc acccctgacg cagccaattc
    14041 aactcttgaa ggaggcgcta cccgccagat gactgggaca ctaccagcac ctgatgaaaa
    14101 tggtcgtctg ccgttaagca gcctgggcag cacaacaatg actacctcca ctcagccatt
    14161 aacgctgaat aacctcatgt ggcagggaga tatcaatggt cttctggatt tgatggccag
    14221 ccgaagcggt ctgtactggc gcatggataa tggtcggatt gtattctatc tgactgaaac
    14281 cagaacgtat ccacttcata tgctgaacac caaaaccagc agcagttcca gcgtcagctc
    14341 tggctcaaca agcacaatgg gggcaacagg aggccaggat aactcagcat ccggtgatgc
    14401 tacgtcctct cagagcacaa ccgtcggtca ggaatacgac ctgtatgaag acatccggaa
    14461 aactattgaa gcgatgctga caccagaaaa aggccgttac tggttatctg catcgagctc
    14521 aacgctaaca gtcactgata ctcctgctgt ccaggaagcc gtcgcacgat atgtggacga
    14581 acaaaacagt attatgaacc gccaggtagc cctgaacgta caggttctga gcgtcagcaa
    14641 taccagaaac gagcagttcg gtctggactg gaaccttgtt tacaaatcgc tacattccgc
    14701 cggagcaacg ttgaacaatg caagcggaga ttttacaggc gctacatctg caggcgtatc
    14761 aattctggat acggcaacag ggaatgccgc caaattcagc ggttccagcc ttctgattaa
    14821 agcactgagt gaacaaggcg atgtcagcgt tgtaacttca caagaaagca cggtcacaaa
    14881 cctgacgccg gtacctatcc agatggcaga tcagacagtt tacgtcgccc agtcagcaac
    14941 aactacgact acggatgtag gagcaacaac aacattaaca ccgggcatga tcaccactgg
    15001 attcaatatg accctgctgc ctttaattca gaaaacaggc aatctccagt tgcagatgaa
    15061 ctttaatctg tctgatcccc caactatccg tagctttacg tcaaaagacg gaaacagtta
    15121 catcgaaatg ccatatacca aactgcgttc actgagccag aaggtcaatc tgaaagaagg
    15181 gcaatcactt gtcgttactg gtttcgatca gaacaatacg acgacaagta aagccggtac
    15241 gtttacgcca gcaaatccat tatttggtgg ttcacaaacc gggaaaaatg aacgcagcac
    15301 gcttgtgatc atcattaccc cgactttccc gtcaggaggc aataatggct gatgaagata
    15361 tcaacccggt agtgcttctc gcagatccta aagtaaatca tcgcgtgtgg gctgcctgtc
    15421 ttaaatggac tcctgtcgta aaaaaacagc gcgtcccctc acatcaaaaa cacaagtctc
    15481 atgtaaaacc cagacggctt acctccctga aagtgactgt aggcagcaga aattcccgtg
    15541 gagagatcag ccgccttacc ggcacaggaa tactgaccag gccagaacgc aatcattatt
    15601 tttccctggc actggctttc tgctcatggg tcagaaatgg atacggtgtc ttccggtaca
    15661 gcgataagga gctcttattc cttgcttcca ttaatggcca gcctgccgtt atggctgact
    15721 tatcaggaaa tgatgccgac gttgcccaaa aggtcagcct ttttctggcg atgaacgaag
    15781 agccccccga aaaatggcag gttgtatcgt ctctggaaca tcctgataac tgggaaagta
    15841 ttattacccg gttatcatca gcagacctcc gccgttgcaa acttactgtc ggcaatcgca
    15901 gcaaattcac acttcctgcc gtactgtttt tagtagcagc cggcgcaggc acagtattct
    15961 ggatgactca gccagagcct gatgtaggac cgaccgccga ggagatagct gcacgggcaa
    16021 gattacagtt taaaaagccc gaaccaccac cggagctccc gcatccatgg gcgtcacaac
    16081 ctgtaatcag tgattttctg aaagcctgcg cggaccttcg aaaaccatca ccggtcgcgc
    16141 tagaaggctg gaaacttacc ggtggaacat gtactcccga gacattcaca ctgatttacg
    16201 aacgacagcc aggaggaact attgaaggct ttctggcaag aagcaaagaa atctttaatg
    16261 ttattcctga ctttaacctt aaagatggtg cacgcctggc ttctgtaacc cggccactgc
    16321 cttccctgcc ccgcagggat gaggcagtcc ctgccccctc agaacaactg atgcgggtgt
    16381 tcacgtggtt tcagaaaaaa cagttaacgc cggccattaa tgaaatagcc atacaggaac
    16441 cattaccagg taacgatggt gaaccagccc ctgttcagaa atggaaagaa tatcagttca
    16501 gtttatcaac gcctgtaaat cctgatgagt tgttcccttt atttcaggat accggcgttc
    16561 gtctgagcaa tatccatttt gaattaaacg gcggtacttt cagttattcc tcagaaggtc
    16621 atatatatgc gtccaggtaa atcactcatt attccatcca tatttttctt ctccggtttt
    16681 tcatttgcaa caacacaacc tcttgtcaca ataggagaac ttgaagcaca acaaaaccgg
    16741 aatattcttt tacaggcaaa agttcagggc gcacagctac agaaacaact cgaagaatca
    16801 gatgttaatc tgtcatcaga aactgtttca ggtttctctg gtgtgactgc ctccttgcca
    16861 tcagtttcag agcagccgac atctaaaaac agaaaggaat tgcccgttat tatggaaata
    16921 aacggcaaag ataaacgtct tcatgccgtt cttcgcatgg ctgatggacg tcagacaagc
    16981 gtcaccactg gtagccagct tccgggtaca tccgtcacgg tgaagtcgat ctcattgtct
    17041 ggcgtcacgt taagtgatgg caccaccctt actttctgag tgatgtgata tggacaaata
    17101 taatctgaag gatgctctcc tttttatatc ccggggagac acccatgaaa ttctcatcga
    17161 aacaaatcag cgaacaagac ctgacgtaca gagtaatctc caggaactgc taaagttata
    17221 ccctgacatc accccaaaag tagtctcgct gtctgagtta caggaagccg gtcaggatga
    17281 tgaaaataaa ggtccgaaag aacggattat tcatctgaaa gatcttgcag atgtcagtga
    17341 aagtgagaag aaagttctct cctattttga aacggccaga aaacttggtg cttcggatat
    17401 tcatttttta atttcagagt ctattttcaa agttcgcatg agaatattcg gtgaactgca
    17461 gactgtagat gaagatcaac ctgcattggg ttattcactt tgcgcaacag ccattctgtc
    17521 aatggctgat gtcactgaaa ccagtttctt ccctcaaagg gaacaggatg cgcgcctgtc
    17581 cccacaatta atgcgaaaaa taggaatatt tggtgcacga tacagtcaca gacctacggg
    17641 agatggtctg attgcggtta tgcgcctgat acccgatgac ggagacaaag ttcccacgtt
    17701 taagcaactg ggctttatac cggagcaaat ccggttgctg aacgtcatgc tgcgccgccc
    17761 ggaagggaaa attgttctgt ctggtccaac aggttcgggt aaatcaacaa cgttacgcag
    17821 tgcctgccgt gtatatcttg acgataatca ggggcgacac ctgctgacga tcgaagatcc
    17881 gctggaagga cagatactgg gggcaacaca aaccccgatt atctgtgaca aatccgacga
    17941 agaggccgtc aaactggcat ggagtcgtgc tatttcatcg gcaatgcgac ttgaccccga
    18001 tgccatcatg gaaggggaaa tgcgcgatct tgtctcgatg atgtcaacca cctacgcggc
    18061 tcagaccggt cacatcgttc tgacgacatt acatacaaac tcagctctgg gtataccgga
    18121 gcgaatgatt acgatgggta tgaatgccga tcttatttgt gatgcgcaat tactgattgg
    18181 gatgataagc cagcgccttg taccaacgct gtgtccctcc tgccgtattc catgggaaaa
    18241 gcgagtacct gaacttagcg aagacgaccg cgcttacctt gagcggcact gcaataaaga
    18301 ttcactctgc agtacagata atatctggtt ccgaaatcct cacggatgct ccgactgtaa
    18361 tcacgacgta attattaacg gcagaaagcg cggtgaaata ggtaaaggcc tcaccggcag
    18421 gaccgtaatt gctgaagtga tagaacctga taatcgctta tttcagattc tgaaaacacg
    18481 cggcaaagtt gcagcacgta aatactggct tgaaaacatg catggcatca gtcgtgtgga
    18541 gcatctgctt cgccgtatca atgaaggcct ggtggaccct cttgaagccg acagaatcat
    18601 ccctctggac gaagacgaac gcctcagcat cgatgatgcc taacaggaga acaacatgcg
    18661 tgaaatgaat ttcagtcaac ggctgagacg tttcattgtc agaaaaacgt tcagcgcccc
    18721 ctacagggtg cagttttacg aagctctgcg ttttctgctg gaaaataaac agccattaaa
    18781 gaccgcactg gaacagatgc gggatgcctg gacagacttt ggacgaaaat ggcacccctt
    18841 tgctgaactg gccacagact gtattgagtc tcttcgtgaa aacagtggcg aaaactctct
    18901 ggaatatact cttggtttat gggttcccca ggaagaggct gcagtaatca gtgccggaat
    18961 aagaagtggt tccattgtgg atgcattaca atttgccacc acgcttacag atgccaaaaa
    19021 gcaactccat caggccatat ggcaaatggc tatttacccg gtgggattgt taatcatgat
    19081 gacggggaca ttatatgttc ttaatacaga gctaatacct gtactgagca aaataagctc
    19141 ccctgattca tggagtggtg ctcttggttt tctctatgga ttatctttgt ttgtcgataa
    19201 ttacggcgct atatgtgccg ttctttttgc agtggtcaca ggtgtaatat catggtctct
    19261 gaaaaactgg aaaagccgct ctttggctga caatataatg ccatggagta tttataagga
    19321 tatacagggg gcagcattcc tcctgaatat ggctgcactt ctgaaagcca aaatgacaac
    19381 gctcaactca ctgaacgttc ttcaggaatt tgcatcacca tggttaagta cacgccttga
    19441 cagcattatt taccgcgtcc gccaggggga tcaccttggg ctggctttac gacagtgcgg
    19501 ttatcatttc ccttccaggg agtctgccaa ctttctgtct ttattacagg gagatggtgc
    19561 aacggagcta atcggcaatt atggccaacg gtggcttgta caaacgctgg agcgtgtaaa
    19621 aaaacgagca gctgttgtgc gtttaattat gctgatattt cttgtcatgt cactcctgtt
    19681 actggtcatg gccgttgtag acattcagtc tattggcgac aacagtatgg gtagtattta
    19741 aataaaacga ggtatttatg ttaattcaaa atattaatgc taacgttctg aagaaaaatg
    19801 agaaagaaac gtctcatgac aaaggctggg gtattatgga acagggtgca atagcacttg
    19861 ttgtaattat cgtacttgcc cttgtttttg gtggattgta tatgttgcgt tcacgaaccg
    19921 gtgtggcaaa tgaaagttcg aacatacaga caatcatcac aagtactcag gggctgctaa
    19981 aaggtagcga tggatatacg tttaccagtg gagccaaaat gacgggtgct ctcattcaga
    20041 tgggtggcgt acccaagacc atgacagtgc gtggtactcc atcatcaggt acagcaacgc
    20101 tctataactc atggggcggt gccgtaactg ttgctccggc ttcaacatca ggtttcaaca
    20161 atggttttac cgtcacctat gaaaaagtcc cacaggatgc ctgtatccag attgccacac
    20221 aaatcagtcg tactggttta actaacggta ttaccctgaa cagtacggct cacagtgatg
    20281 gaaaagtcac tactgaagaa gccagtgcgc aatgcacagc tgataatggc agcaccggta
    20341 caaacaaact aatcttcacc atcaatggct aaggagtttt aatgtccggg ggtaaagtgc
    20401 ccctcagact tatttcatta atcctgcttt gttctcttat ttatcccgcc cgagcttcat
    20461 acatcgcccc caaacaaatc tggactaaca agtgggacaa ttgctttgcc gccgccggcg
    20521 cacgctatca aattgaacca ttattgttga aggccatttc tgccggggaa tcttcattga
    20581 agccgggcgc aattaacata aataaggaca gaaaaacagg caaagccagt agtacggatt
    20641 atgggttgat gcaaataaac tcaacccata ttccaaagct tataaagatg ggcgtaatca
    20701 aaaagtctga ggatttaatt accaaaccat gtcttaatat ccatatagga agctggatac
    20761 tggcaagaca ttttcagata tgtggcgtca gctggaattg ccttgggtca tacaatgccg
    20821 gattccgtaa ggacagacat gaaacaagag agcaatatgc taataaagta tggagaattt
    20881 atcgtgacat gaaagggata tgccttcccg ggcagggagg cagacaatgc agacaatcgt
    20941 aggcataagt ctctcccctg tttatatcct cccattgatg ttcatatttt ccattattgg
    21001 acgaactctg ttattcagag ttcgttattt tttatctgat acagaacatc tctggtataa
    21061 gacacacact gcagttctgt caggaatatg gctatatagt atagctgtgg cattaattat
    21121 tttatcgtca tccccactta tctaccgtat tcatgctgtt cttattcttt cgttcatgct
    21181 gcaaatggca gtaacggatg cactaacagg cctcttaccg ggtacattca ccaggcgttt
    21241 tttaatcgct ggaatattat cacaaatcac aactgatata tggtggctcc gtgcaacaga
    21301 aattgtctct gccgcaattg tcttattctg tctgcataaa ctggttaacc gccatcgtct
    21361 taatattggc acaggagacc tgtggcttat tgcaggtatt accgcatggt cagggctata
    21421 taacgccata tggagtgtgt tgctgggcgc aggaggattt gtcttatggc attcaacatg
    21481 gtgcataaaa ggacataaag aaggcccact gggtccatgg ctgtgtcttg gccatgtcct
    21541 tttgctactg gataatcttt atcaaccact atgggtaatc tgatgaaaaa atatgaccga
    21601 ggctgggcat cgctggaaat tggcgcggct ttattgattg ttatgatcat agttgcctgg
    21661 ggtgcaggca tatggcagga ctatttaaaa accaaaggtt ggcaggctga agctcgtctt
    21721 gtcagcaact gggccagtgc agcccgctct tatataggga aaaactacac aactctccag
    21781 gccagtagta ccacaacaac ccctgccgtt atcacgacaa ccatgctgaa aaataccggc
    21841 tttttgtcca gcggctttac tgagacaaac agcgaggggc agcggttaca ggcatatgta
    21901 gttcgaaaca cccaaaaccc ggaattacta caggcaatgg ttgtatccag tggtggcacc
    21961 ccttatccag tgaaagcact tatccagatg gctaaggata ttaccactgg tcttggtgga
    22021 tatatccagg acggcaaaac agccacaggt gcattacgtt cctggtcagt agctttaagt
    22081 aattatggtg ccaaaagcgg taacgggcat attgccgtac tgttatcgac agatgaactt
    22141 agtggtgcag cagaggacac tgatcgtctt tacagattcc aggtcaatgg tcgccctgac
    22201 ttaaacaaaa tgcacacggc cattgatatg ggatcaaata acctgaataa cattggggca
    22261 gtaaatgccc agactggtaa tttcagcggc aatgtgaatg gtgtaaatgg cactttcagc
    22321 ggtcaggtaa aaggcaatag cggaaacttt gacgtaaatg tcaccgctgg cggtgatatc
    22381 agaagtaata atggttggtt aattactcgt aacagcaaag gctggctcaa tgaaactcac
    22441 ggtggcggat tttatatgtc cgacggatca tgggttcgaa gtgtaaacaa caagggcatc
    22501 tataccggcg gtcaggtgaa aggcggtact gttcgtgctg atggtcgcct ttatactggt
    22561 gaatacttac aactggaaag aactgccgtt gctggcgcat catgttcgcc taacggcctt
    22621 gtaggccgcg ataatacagg ggcaatactt
//
LOCUS       DAEVZA010000080        22610 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEVZA010000080 DAEVZA010000000
VERSION     DAEVZA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.080"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            372..1208
                     /locus_tag="LAF92_003059"
     CDS             372..1208
                     /locus_tag="LAF92_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBI9792026.1"
                     /translation="MFNLLDSLYVESVDTLTFKYQSIIKGLDRLFEGINLDSAFSLSE
                     EERSYFEEKAKSLNALAQLGISVEIISHELEEMDSMVTRGLNSLPTSVKEHPGFSLAL
                     NAHRLLTQQIRFLSPLKISGYQSRQRITGKNIMDYVLKFFGERFERQRITIEFSEEFK
                     QIAITDIPSRIYPVFTNIINNAMYWVSLSNNRLIKIGFVNSLVIIANSGPAIDTDDIP
                     RLFELFYSKRANGHGVGLYLCRENLAVAHHKIWYSEPDEGDNYLIKDGANFVIQFNGV
                     EF"
     gene            1210..2958
                     /locus_tag="LAF92_003060"
     CDS             1210..2958
                     /locus_tag="LAF92_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792027.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSDLIIVDYHLDNNAPD
                     KTLKLLQDLKDSDHLNMIVIYTRENLETVWMQISSTLKGALDINSLIIDYDNEDVQSY
                     WEDVVLPNLNDNGNKALTRDETIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMI
                     AEYAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTL
                     NDSLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEILKSDSPDIRC
                     RNIDFVFGNLSEELYQRLKNNNTLDEFIKSVFDSYSNEYANSGVAALLQYCSSKMDLP
                     SNNDTYHEMYHALNMNLSSKNFEDGHISTGTIFFDTESNKWYLCVSAACDLVPTQGND
                     PHHVRLSPHRLIKVLELFNASQSKALPFAEHSKYIYVMHKNQRKYLSIFEGDKTLPVV
                     DYMVVLNHGTTVDGEEKNIISAVFLSNMDGNVQNVPVRLKLKSQLRTGYAERYQAIAS
                     QYSSRIGVDYVSMMLP"
     gene            complement(3030..3446)
                     /locus_tag="LAF92_003061"
     CDS             complement(3030..3446)
                     /locus_tag="LAF92_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792028.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLLLFSLECMKLRRLKQK"
     gene            complement(3485..4714)
                     /locus_tag="LAF92_003062"
     CDS             complement(3485..4714)
                     /locus_tag="LAF92_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI9792029.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(4880..5242)
                     /gene="ssrA"
                     /locus_tag="LAF92_003063"
     tmRNA           complement(4880..5242)
                     /gene="ssrA"
                     /locus_tag="LAF92_003063"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5457..5939)
                     /gene="smpB"
                     /locus_tag="LAF92_003064"
     CDS             complement(5457..5939)
                     /gene="smpB"
                     /locus_tag="LAF92_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBI9792030.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            6110..6547
                     /gene="ratA"
                     /locus_tag="LAF92_003065"
     CDS             6110..6547
                     /gene="ratA"
                     /locus_tag="LAF92_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBI9792031.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            6537..6827
                     /locus_tag="LAF92_003066"
     CDS             6537..6827
                     /locus_tag="LAF92_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBI9792032.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(6889..7230)
                     /gene="bamE"
                     /locus_tag="LAF92_003067"
     CDS             complement(6889..7230)
                     /gene="bamE"
                     /locus_tag="LAF92_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBI9792033.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(7379..9040)
                     /gene="recN"
                     /locus_tag="LAF92_003068"
     CDS             complement(7379..9040)
                     /gene="recN"
                     /locus_tag="LAF92_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBI9792034.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLTLAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(9126..10004)
                     /gene="nadK"
                     /locus_tag="LAF92_003069"
     CDS             complement(9126..10004)
                     /gene="nadK"
                     /locus_tag="LAF92_003069"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBI9792035.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10127..10720
                     /gene="grpE"
                     /locus_tag="LAF92_003070"
     CDS             10127..10720
                     /gene="grpE"
                     /locus_tag="LAF92_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBI9792036.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(10775..12061)
                     /locus_tag="LAF92_003071"
     CDS             complement(10775..12061)
                     /locus_tag="LAF92_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBI9792037.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVHIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12082..12873)
                     /locus_tag="LAF92_003072"
     CDS             complement(12082..12873)
                     /locus_tag="LAF92_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBI9792038.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            13040..14401
                     /gene="ffh"
                     /locus_tag="LAF92_003073"
     CDS             13040..14401
                     /gene="ffh"
                     /locus_tag="LAF92_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBI9792039.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            14650..14898
                     /gene="rpsP"
                     /locus_tag="LAF92_003074"
     CDS             14650..14898
                     /gene="rpsP"
                     /locus_tag="LAF92_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBI9792040.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            14917..15465
                     /gene="rimM"
                     /locus_tag="LAF92_003075"
     CDS             14917..15465
                     /gene="rimM"
                     /locus_tag="LAF92_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBI9792041.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            15496..16263
                     /gene="trmD"
                     /locus_tag="LAF92_003076"
     CDS             15496..16263
                     /gene="trmD"
                     /locus_tag="LAF92_003076"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBI9792042.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            16305..16652
                     /gene="rplS"
                     /locus_tag="LAF92_003077"
     CDS             16305..16652
                     /gene="rplS"
                     /locus_tag="LAF92_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBI9792043.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(16729..17211)
                     /locus_tag="LAF92_003078"
     CDS             complement(16729..17211)
                     /locus_tag="LAF92_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBI9792044.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(17227..18453)
                     /gene="dgcN"
                     /locus_tag="LAF92_003079"
     CDS             complement(17227..18453)
                     /gene="dgcN"
                     /locus_tag="LAF92_003079"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBI9792045.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            complement(18443..18961)
                     /locus_tag="LAF92_003080"
     CDS             complement(18443..18961)
                     /locus_tag="LAF92_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBI9792046.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            complement(19111..19476)
                     /locus_tag="LAF92_003081"
     CDS             complement(19111..19476)
                     /locus_tag="LAF92_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBI9792047.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            19686..20756
                     /gene="aroF"
                     /locus_tag="LAF92_003082"
     CDS             19686..20756
                     /gene="aroF"
                     /locus_tag="LAF92_003082"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBI9792048.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            20767..21888
                     /gene="tyrA"
                     /locus_tag="LAF92_003083"
     CDS             20767..21888
                     /gene="tyrA"
                     /locus_tag="LAF92_003083"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBI9792049.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(21931..>22610)
                     /gene="pheA"
                     /locus_tag="LAF92_003084"
     CDS             complement(21931..>22610)
                     /gene="pheA"
                     /locus_tag="LAF92_003084"
                     /EC_number="4.2.1.51"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="catalyzing the formation of prephenate from
                     chorismate and the formation of phenylpyruvate from
                     prephenate in phenylalanine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBI9792050.1"
                     /translation="SSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINT
                     VYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGL
                     QVLERIEANQQQNFTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNH
                     NLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSEN
                     VVPVDPT"
BASE COUNT         5887 a         5035 c         5637 g         6051 t
ORIGIN      
        1 tagatgcgtt gcgcagtgaa attaaaacgc ctatcaaacc tccaaaactt ggaatgtatg
       61 aagagaaata tagagactac agagataaat ttaatgagtt ctctgcgtat attctacaaa
      121 tgaagttagc aattaataaa cttgattctg aattaaataa acttgagcct tcattgtcag
      181 cgaaaaatca ccttgaaaaa aatcaaggta ttattaattc taaactaact aagtttaata
      241 acacgataga ggagaagata cattctcttt taaaaaaatg ggccgatgaa ataaaggttg
      301 atcgaagtga ttattatgct aaaactatat cagttgttga ttcaatagat aatgattcac
      361 aaattgaaaa tgtgtttaat ttgctcgata gtttatatgt tgagtcagtt gataccctaa
      421 ctttcaaata tcaatcaata ataaaaggtc tcgatagatt atttgaaggt ataaacttag
      481 attcagcatt ctcattatct gaagaagaac gctcatattt tgaagaaaaa gctaaaagtt
      541 taaacgcgct tgcacagtta ggtattagtg ttgagataat atctcatgaa cttgaagaaa
      601 tggattctat ggtaaccaga ggactaaact ctcttcctac ttctgtaaaa gaacaccctg
      661 gtttttcatt ggcgttaaat gctcacagat tgcttactca acaaatacgt ttcttatcac
      721 ctttgaaaat atcaggttat caatccaggc agagaataac tggaaaaaat atcatggatt
      781 atgtcctgaa gttctttggg gagcgtttcg aacggcaacg aataactatt gaatttagtg
      841 aagagtttaa gcaaatcgca ataacagata taccatcaag gatctatcct gtttttacta
      901 atattatcaa caatgcaatg tattgggtca gtctgtcaaa taataggctc ataaagattg
      961 gttttgtgaa ttctttggtt atcatagcaa attctggtcc ggcaattgat accgatgata
     1021 tcccgcgact atttgaacta ttttatagca aaagagcaaa tggacatggg gtaggtctgt
     1081 atctatgtcg agaaaacctt gctgttgcac atcataaaat atggtattca gaacctgatg
     1141 aaggcgataa ctatttaata aaagatggcg ctaattttgt gatccagttc aatggagtgg
     1201 agttctaata tgacagtggc aaattataat tctcttgtcc agaaaacttt ctgcgaaaat
     1261 gcaattcgtt ccgttgtcat gattgatgac gattttctga cgtattctga atcaatcagg
     1321 gcgttgaata acgaagttga tttagactac aacaaaattg actcatctaa acgagccgct
     1381 actcttgaga gcttttttca atctaaaaat atgatttgtg atgttgacaa tggttctgtt
     1441 aatttcgatg tggatcggat tagaaaatca gatcttatta ttgtagatta tcatcttgat
     1501 aataatgcac ctgataaaac acttaaacta ttacaagatt tgaaagactc cgatcattta
     1561 aatatgattg taatatatac tagagagaat ttagaaacgg tttggatgca gatatcatcg
     1621 actctcaaag gtgctctgga tatcaacagc ttgatcattg actacgataa tgaagatgtc
     1681 caaagttatt gggaagacgt tgtattaccg aacttaaatg ataatggtaa taaagctctc
     1741 acaagagatg aaacaatagc ctatattaaa gacagtaagc cttgtagaag aattaaaaga
     1801 ttaatacatg atgatgctgt gttggaggat caaaaggata aaaacttcat tgcaaaaatg
     1861 attgcagaat atgctgtgtc tagaaatgca attatttcta gcaacacatc tggcaatgtc
     1921 attcggggtg atgaaagcgg agtaaaatgg attcaatgtg gtaatatctt tgtctcccta
     1981 tttcataagg ttcaagatga tcatgaaaac gatggagata ggatttggca aactctcaat
     2041 gattctctca ttgaatggaa accatcttat tatcagttaa taaaatctga aattcagaat
     2101 gcaatcgaag ctgaggcttt atcttttgta aatcatttgg ctaacgatca ttacggtcaa
     2161 gctgcgtggt taaatgagat attaaaatca gactcgcctg atattagatg tagaaatatt
     2221 gactttgtat ttggtaattt atcagaagag ctttatcaaa gacttaaaaa taataatacg
     2281 ctggatgaat ttatcaaaag tgtttttgat agctattcaa atgaatacgc taacagcgga
     2341 gttgctgcat tgctccaata ttgctcttca aaaatggatc tgccatcaaa taatgatact
     2401 tatcacgaaa tgtatcatgc tttaaatatg aatttgtctt caaagaattt tgaagatggt
     2461 catatttcta ctggcactat tttctttgat acagagtcga acaaatggta tttatgtgta
     2521 tctgcggcat gtgatttggt tcctactcag ggtaacgacc ctcaccatgt aagattaagt
     2581 ccgcacaggc tcattaaagt tctggagctt tttaacgcca gtcagagtaa agcattgcca
     2641 tttgctgaac attcgaaata tatatatgta atgcataaaa atcaaagaaa atatctctct
     2701 attttcgaag gggataaaac gcttcctgtt gttgattata tggtggtgtt gaatcatgga
     2761 acaacagttg atggcgaaga aaaaaatatt atttctgccg tgtttttaag taatatggat
     2821 ggcaacgtgc aaaatgttcc tgtccgactc aaacttaaat ctcaactgag aactggttat
     2881 gcagaaagat atcaggctat agcgtctcag tatagctcaa ggattggtgt ggattatgta
     2941 tcaatgatgc taccataatt attatatttt aggcgtggtg attttttttc gccatgccta
     3001 ttttatattt atcatcacaa tgatgttgtt tatttttgtt taagtcttct aagcttcatg
     3061 cattctaatg agaataataa tagagttgtg ctgtatataa agccagagtt agttaataac
     3121 tttaaattta atgcttcagg attttcactg agaaagtgaa aggcataaac taagtataca
     3181 gctaaagtag ccgttccaat tgttggtcca atatcgcctt gatcatgagg ttcaaggttt
     3241 acattgaact tgaagattag ccattcaaat ccccaagtaa aaaaaacaac taatatcatg
     3301 gctgcgaata atttcgcgat gttatctgct gatggattca tcagaacaga ttgtttctga
     3361 aaaaattcac aaagtgagag tccgaaaaaa ataaaaaaaa gtggtctgga actaaatttt
     3421 tcaaaaactt tgaatagggt ttccattttc tttaccttca ggattatatt agctattctg
     3481 atacttaggc taccagagca tttgatcttt gagcttgaac gtaatcactc caccattgca
     3541 tcaaactctg tcgctctatc agatattctg cacgattgta tgctgcgata atttcatctt
     3601 ttttcgagtg ggcaagcgct gcctcaagaa cttcagctct gaatttacca gactcctctg
     3661 ccgctgttcg tgcaatagaa cgcataccgt gagctacaag ctcgcctccg aaccccattc
     3721 ggatgatagc tgcgttggct gtttgttcat gcatatgatt aagaggcgct tttatgctgg
     3781 ggaaaaccca ttctctatgc ccacttattg atttcattaa ttcaaggatg cgcaaagctt
     3841 ctttactcaa aggaactttg tgaagctttt tcattttcat gaaatcagca ggaatgttcc
     3901 aaatgctgtt ggttgtatca atatcagacc accttgcgcg aacggcttca cccggacgaa
     3961 cccatgtcaa caattgccat tcaattagca tacgtgtttc caaccggatt gacgcattcg
     4021 tcaaagattc cataaacctt ggcaattcgc ttgggggaag ggcaggcata ttttgctttt
     4081 ttggtttact gaatctttga ccaaggttgt cagccgggtt gaactcaata agttcttcag
     4141 tagctgccca ccggaagatt tcattcagac gtgaaatgat acggcgtaga gtttccaata
     4201 cgcctcgttg ctcaatagga tcaaggtgtt gttttaagag cttaggtcgg atctcattga
     4261 tagggacatt acccagaccg ggaaagacat ttctctctaa gctgcgccag atgtctgctg
     4321 catggtcttg tgagatacct gatgtcttta ccttctcatc taaccatttc cgcgctacgg
     4381 cttggagagt gtgctcagta gcactcttta atgccttcgc cttatcgttg ttatggattt
     4441 ggggatcaac accatttgcc agaaaggaga gatattcatc acgtaaggct ctggctcttg
     4501 caagggtaag gtgaggatat gtcccaaggc tcattttggt tcttttcttg ctcactggta
     4561 ctgcatacct gaaataccaa tttttcttgc ctcctttcgc caaaggagcg attcgtagaa
     4621 tcagaccatc accgtcaaac aagttgattt ctttatcggc tggcttggtg cttttgattt
     4681 cagtgtcagt gagcttctta gcgatttttg ccattttggg accctcggtt tttggaccct
     4741 tcttagtggg tcccattcag ggtgccataa ctcgtagttc tcagcaattc tcactggacg
     4801 acaatagacg taaaaaagcc cgcagagctt gtgctgtgtg ggcttagtag acttcattga
     4861 acttcaaaca actaaaaagt ggtggagctg gcgggagttg aacccgcgtc cgaaattcct
     4921 acatcctcgg tactacatgc ttagtcagtc tttacattcg cttgccagct gcggacggac
     4981 acgccactaa caaactagcc tgattaagtt ttaacgcttc aaccccaggc agggcttcca
     5041 cgcgatctct tttgggtttg acctctcttg atccccgtcc taagagcgga ggctagggag
     5101 agagggctct aagcaggtta ttaagctgct aaagcgtagt tttcgtcgtt tgcgactatt
     5161 ttttgcggct ttttacgagg ccaaccgccc ctcggcatgc accttgggtt tcgcaaatcc
     5221 cgtcgaatcc agaatcagcc ccaatgtgta aaggtaagta taccagattt atgagcgcca
     5281 tgaccagcct caatggcgtt atcgttaaag atttagcacc catgtagcct gatttttatt
     5341 cgattaagca atgggatggc aacatttgtg tcggatgtga tagccaataa gatgttcatt
     5401 cgcgccgccg gagagggagg cgcggtgagg aactggtcaa taattggagt gcaggtttaa
     5461 cggtgggcgt ttttcatgat acgtgcttta tccacctgcc attcgcgttc tttgatatct
     5521 gaacgtttat cgtgctgttt tttacctttg gcgacgccga ttttcacttt gcaccaggca
     5581 tttttccagt acagggagag cgccactacg gtatagcctt ctcgattgac gcgaccgtac
     5641 aatgagtcca gttcgcgctg gttgagaagt aacttgcggg tacgggtagg atcgcacacc
     5701 acatgcgtgg aggccacggc cattggcgtg atgttagcgc caaacagaaa tgcctctccg
     5761 tcacgcagaa ggacgtagct gtcgctgata ttggcttttc ctgcgcgcag ggatttaacc
     5821 tcccagcctt gcagggcaag tcccgcttcg aactcttctt cgataaagta ttcgtgacgg
     5881 gcgcgcttgt taagcgcgat ggtcgctgaa ccaggtttat gtgctttttt cttcgtcata
     5941 agcgtcgtga atcatcggta atctgaaatc gaaaacaccc catatcaatc ctgcggggga
     6001 caaggctcta tcttagcatg aacccgatgt tacccagcgc cgggatagcg ttttttttac
     6061 agcaggataa atgatattat ttgttggatt tttgttgatg gaaattgtta tgcctcagat
     6121 tagccggacc gcactggtac cctacagcgc ggagcaaatg tatcagttag tgaatgacgt
     6181 tcagtcttat cctcagtttt tgccgggttg taccggaagt cggattctgg agtccactcc
     6241 tgggcagatg actgcggcgg tagatgtctc taaggctggg atcagcaaaa cgtttactac
     6301 ccgcaaccag ttgaccagta accaaagtat tcttatgaat ctggtggatg ggccgttcaa
     6361 gaaattgatt ggtggatgga agtttacgcc gctgagccag gaggcgtgtc gtatcgagtt
     6421 tcatctcgac tttgagttta ccaataagtt gattgaactc gcctttggtc gcgtgtttaa
     6481 agagctggcg tctaatatgg tccaggcttt tacggttcgt gcgaaagagg tttacagtgc
     6541 caggtaaaat tgccgttgag gtggcttatg cgctacctga gaagcagtac ctgcagcgag
     6601 tgacgctgca ggagggcgcg acggttgaag aagctattcg cgccagtggc ttgctggaat
     6661 tgcgtaccga tatcgattta actaaaaaca aagtcggcat ttacagccgt ccggcaaaac
     6721 taagcgatag tgtgcatgat ggcgatcggg tggagattta tcgccctctc attgccgatc
     6781 cgaaagagct tcgcaggcaa cgagcagaaa aatcagcgaa taaataacag acagaaaaaa
     6841 ggtgctcatt gagcaccttt tttaacgtct ttgagagcaa ctttattatt agttaccact
     6901 cagcgcaggt ttgttatcaa tattggtcaa cacaccgcta ctgttaaagg tcagcgtcag
     6961 cgtttgctgc gttacacctt catgacctgg ttgctggcgg aagacataga accaggtatt
     7021 cgtaccaaat ggatcggaca tcagcggtgt acccaatgcg tacgcaactt gttgttgcgt
     7081 catgccaaca cgtattttgg atacgtcgtt agcggtcaga tagttcccct ggttgatgtc
     7141 aggacggtaa accactcgct ccagagtgga acagcctgcg gtcaacatca atagtactgc
     7201 tgctgcagca gtcagcgttt tacagcgcat agtgatttga ttccttttcg ggcccgagca
     7261 gtacgtggct catctgtaat atgccgatga taatagacct ttcatcactt taaaaccttt
     7321 tgctttccgg tcttacggcg ttttgctgtt tactctgacc gtgaagcagg aaaaaagttt
     7381 acgctgcaag cagttctttc gcattcgcca gtgtattacg tgtgacttca ctgccaccaa
     7441 gcaggcgcgc cagctcttgt aaccgcgctt ttttattcag ggattgcata tgcgtttctg
     7501 tcatcgcacc atcggtttct ttgctgacaa aatagtgttg atgaccacat cccgcgactt
     7561 gtggcaggtg ggtaacacac atcacctgag ttgattcgcc gagttgacgc agcagtttgc
     7621 cgacaactgc cgctgttgga ccgctaatcc ctacatccac ttcatcaaaa atcagtgccg
     7681 gggtttccat tttacgcgcc gtgatgacct ggattgccag tgcgatgcgg gacaattcac
     7741 caccggatgc gactttggca ataggctgca ttggctgacc tgggttggtg gttacccgaa
     7801 actcaatacg atcggcaccg tcagcgccca ggtgatgctc gtcaaattta acatcgatcg
     7861 taaactgccc atgcggcatt gagagcgcat gcatactgtc ggtgatcagc tgtgcaagtt
     7921 cttctgcata ttgctggcgt tgttggtgta atgcgcgcgc gatttccagt gcctgctgat
     7981 gatgtttcgt taccgccagc gtaagcgttt cttgtgagtc ggcctgatcg tccagttgct
     8041 gctgttcttc cagtagcgac tggtaatact gtggcaatgc ctcagggctg acgtgatgtt
     8101 tacgtgccag cgaaatctgt tttgagatgc gctgttcaag ttcaaatagt cggttgggat
     8161 cgagatccag acgatcgcag tagtggcgca gttcatcgct ggcttcagca atctggatgg
     8221 tagcttcttc cagcatatca agtacgccgg acagtttgct gtccatgcca atcaattcgc
     8281 tcaccagttg tttagccgtg taaagctgac tttgcaggtt tgcgtcttca ccgtcggcca
     8341 ttaatgccaa tgcattctgg ctggtggtca gcaattgacc gctgttcgcc agacgtttgt
     8401 actcttcgtc gatttgttca aactctccgg gctgcggatt aaattcgtta agttctttta
     8461 attggtattg cagcagttcc gcacgggcag cgcgttcctg acttaactgt tgatgatgcg
     8521 cgaggtcacg gcagctttga tgccacaact gataacgtgc tgtcatttcc tggagtagag
     8581 aggtttcatt ggcatagcca tcaagcagga acttttggtg ctcgggtttg gtgagtaact
     8641 gatgagcgtg ctgaccatgg atctgaatca gcaactgacc cagttcgcgc agttgtgaca
     8701 gaggaacagc tgtaccgttg atgaaaccac gggagcgacc atcgctgctg atcacgcgac
     8761 gaagcaaaca ttcatgcccg tcttcaagct ggttttcttc cagccagcgc agagccgctg
     8821 gcgtatcttt cagagaaaaa cgggcgcaca ggtcagcgcg agcagcgccg gtacgcacca
     8881 tgtcggcttc agcgcgacca ccgagacaaa gaccgagggc atctattgca atagatttac
     8941 ccgcgccggt ctcgccagtt attacggtca tgccgctatg aaaatcaatc tcaagctcac
     9001 gaacgatagc aaagttgctg atggtcagtt gtgccaacat agtcgttttc ctgtatgaaa
     9061 aaccattact gttattgtgt acagtataaa ctggttttat atacagtaaa gaggctggcg
     9121 taaaattaga ataatttttt tgaccagccg agcttggtgc ttaatgtgtt gaaataactg
     9181 taatctttcg gatgaatcag attcagatgg taatcacagc gacgaatcag gacatcttca
     9241 ccttcctgaa tcggcagtgc tatctggctg tcgcaactga tttccaggtc gttacggcga
     9301 tgcgaaaaac gcagacggat cgtgctgctg ctgtttatga ccagtggtcg tgctgacaac
     9361 gtatgcggga acatgggcac cagggtaatc gcatccagag agggggtcag aataggaccg
     9421 cctgcagaga gggaataggc ggtggagcct gttggcgtcg aaataattag tccatcagat
     9481 cgctgagaaa acgcaaagat ctcgtcgata tacacttcga actcaatcat atgcgccact
     9541 ttgcctggat gaagcaccac ttcatttatc gcggtgctga tgcgtttctg gcaatcttgc
     9601 tgacagactt gcgcttccag caaaaaacgt ttctcgctga tgtagtggcc ttccagcaca
     9661 tcggctaact gttgctgggc gttatcgggg tcaaggtcag tcaggaaacc caggttgcca
     9721 cggttgattc caataacttt aatatcgtaa cgggcgagtg tgcgtgccgc gcccagcata
     9781 ttaccgtcgc caccaacgac taccgcgaga tcagctagtt gcccaatctc cgcgagcgtg
     9841 ccagttttca cattcttcag ttgcagttcg tgagcgattt gttgctcaac gatgacctcg
     9901 taaccttttg tgcacagcca gcggtagagc atttcatgtg ttgtcagtgc agtggggtgc
     9961 cgtgggtgtc ccacaatgcc aatacacttg aaatgattat tcatttttcc gaggtccttg
    10021 ttacgaagat tgatgacaat gtgagtgctt cccttgaaac cctgaaactg atccccataa
    10081 taagcgaagt tagcgagatg aatgcgaaaa aaacgcggag aaattcatga gtagtaaaga
    10141 acagaaaacg cctgaggggc aagccccgga agaaattatc atggatcagc acgaagagat
    10201 tgaggcagtt gagccagaag cttctgctga gcaggtggat ccgcgcgatg aaaaaattgc
    10261 gaatctcgaa gctcagctgg ctgaagccca gacccgtgaa cgtgacggca ttttgcgtgt
    10321 aaaagccgaa atggaaaacc tgcgtcgacg tactgaactg gatattgaaa aagcccacaa
    10381 attcgcgctg gagaaattca tcaacgaatt gctgccggtg attgatagcc tggatcgtgc
    10441 gctggaagtg gctgataaag ctaacccgga tatgtctgcg atggttgaag gcattgagct
    10501 gacgctgaag tcgatgctgg atgttgtgcg taagtttggt gttgaagtga tcgccgaaac
    10561 taacgtgccg ctggacccga atgtgcatca ggctatcgca atggtggaat ctgatgacgt
    10621 tgcgccaggt aacgtactgg gcattatgca gaagggttat acgctgaatg gtcgtacgat
    10681 tcgtgcggcg atggtaactg tagcgaaagc aaaagcgtaa tttctgtttt cgtaataatt
    10741 cacggccctg catgcgaatg cagggccgtt ttcgttactc cgccacactc tcgcgcagcg
    10801 gttttacagg aaagactttt acctgcttaa tcatattgtc ctgaacgtcg agaatatcga
    10861 tatcgtactc gccaatatgc acgcgggtgc cagcgacagg gatctcttcc agagcttcaa
    10921 gaatgacgcc attaaccgtg cgggcatcgt cttcaggtag atgccagtta aaggctttgt
    10981 tgatttcccg cacgttggcg gtgccatcga taatcaccga accgtcgttt tgcggcgtga
    11041 cctcttcggc aagtgttggc gacatcgacg tagtgaaatc gccgacaatc tcttccagaa
    11101 tatcttcaac cgtcaccagc ccctgaatgt ctccatactc gttgacgacc aggccgactt
    11161 tctttttgtt gcgctgaaac tttaccaact gcgtgctgag cggcgtacct tccggcacaa
    11221 aatagatctc gtccgcggcg cgcagcatgg tttctttggt gaactctttt ttctccgaca
    11281 tcaaccgcca ggcttcacgt actcgcagca tactgatggc gtcgtccagc gaatcacggt
    11341 agagcacgat gcgcccgtga ggtgagtggg agagttggcg cagaatcgat ttccagtcat
    11401 cgttgatatc aataccgata atttcactgc gcggcaccat gatgtcatca acggtcattt
    11461 tttccagatc gagcaccgac agcagcatat cctgattgcg acgggaaatt tgtgagcgtg
    11521 attcgtgcac gatagtgcgc aactcttctt tgctcaaaga gccgctaacc acgatatcgg
    11581 ttttgatacc catcatgcgc atcagcatac gggtgatagc attcagcaac cagaccagcg
    11641 gcatcatcaa aatttgcagc ggagccagca gaaaactact cggataagcg actttttccg
    11701 ggtacagcgc ggcaatggtt ttcggcaata cctcagcaaa taccagtacg acaaaagtca
    11761 gcacaccagt cgcaattgcc acgcccgcat cgccgtacaa acgcatccca acaatagtgc
    11821 cgagcgcgga ggcaagaata ttgaccaggt tattgccgat taacaccagg cttatcaggc
    11881 ggtctggctt acgcagcaat ttttcgacgc gtttggccga gcgattaccc tgtttcgcca
    11941 tatgtcgcag acgatagcgg ttgagggtca tcattccggt ttcggacccg gaaaaatagg
    12001 ctgaaatgac caccatgatg atcagaataa tgatcaacgt agtagtagaa atgtgttcca
    12061 ggggaaactc cttttctggg tttagctgat taactgctgg acaattcggc tgccgaagta
    12121 ggccagtgtc agaattaccg cgcccgcaac gttaaaccag acgacgcggc gtccacgcca
    12181 tccttcatgg taatgtcccc acagcagcac aatatagaca aaccacgcca cgatagagag
    12241 cacagccttg tcgatatttt ccatgctaaa taggttgtgc atgtagaaca ggccagtgca
    12301 aagcgtgagc gttagcagca ccacgccaat ctgcgtgatg tggaacattt tacgctcgat
    12361 actcatcaat ggcggcattt cctggttaaa tgccagcttc ttgttcttca gttggtaatc
    12421 aatccacgcc agttgcagcg cgtacagggc ggcgataatt agcgtggcat aggaaaagag
    12481 cgataagcca atgtgcacca gcatcccagg cgttgcttcc agatgggtga tgtattcatt
    12541 gggcatgaag gtcgccagcg ccaggttgat aagcgcaaag gcatagacaa tgggtagcag
    12601 caaccagcca cgattgcgag aagccacaat ggtcattacc gtacagatca tcaaactgac
    12661 caatgaacca acgttcagca ggctgaggtt ttgtccgcta tcaccgtcgg gcaggatgcg
    12721 ggcttccaga gcgattgcgt ggcagaccag cgcaatgacc gcagaaataa tagccatgcg
    12781 ccgccagccg ccgttttttt gcagcagacc gggaacaatc agcgcaagac tgacggagta
    12841 ggcgacaagc gcgagcagag caaaaacggg catagtgatg tcgacagttt gagcaataag
    12901 aaagagaagc agtatagcgt caggtgaacg ctgctccaac cgttgcataa caacaaagac
    12961 gccttcatgt tatactgcgg caaaatactg atgatgtgtc gcgattgcgg ccaaccgttt
    13021 ccaccccagg cgagagacaa tgtttgataa tttaaccgat cgtttgtcgc gcacgctgcg
    13081 caatatcagt ggccgtggac gcctcactga agacaacgta aaagatacgc tgcgcgaagt
    13141 gcgcatggcg ctgctggagg cggacgtcgc tctgccggta gtgcgtgagt ttatcaatcg
    13201 cgtaaaagag aaagcggttg gtcatgaagt taataagagc ctgacgccgg ggcaggagtt
    13261 cgtcaaaatc gtccgtaacg aactggttgc ggcgatgggc gaagagaacc agaccctgaa
    13321 cctggcggcg caaccgcctg cggtcgtgct gatggcgggc ctgcaaggtg ccggtaaaac
    13381 aaccagcgtt ggtaaactcg gtaagttcct gcgcgagaag cacaagaaga aagtgctggt
    13441 ggtttctgcc gacgtttatc gcccggcggc aatcaaacag cttgagacgc tggcagagca
    13501 ggtgggcgtt gatttcttcc cttctgatgt tggtcagaag ccggtagata tcgttaacgc
    13561 ggcgctgaaa gaagccaaac tgaaattcta cgacgtgctg ctggtggata ccgctggtcg
    13621 tctgcacgtt gacgaagcga tgatggacga gatcaaacaa gtccatgcgt cgattaaccc
    13681 ggttgaaacc ctgtttgtgg ttgacgccat gaccggtcag gatgcggcca atacggcaaa
    13741 agcattcaat gaagcgttac cgcttaccgg cgtagtgttg accaaagtgg acggcgatgc
    13801 ccgcggcggt gcggcgctct ctattcgtca catcactggc aaaccgatca agtttctcgg
    13861 tgttggcgag aaaactgagg cgctggagcc gttccatccg gaccgcatcg cgtcgcgtat
    13921 tctcggcatg ggcgacgtac tgtcgctgat cgaagatatc gaaagcaaag ttgaccgcgc
    13981 gcaggcagag aaattagcca gcaagctgaa aaaaggcgac ggcttcgatc tcaacgattt
    14041 tcttgagcaa ctgcgccaga tgaaaaatat gggcggcatg gctagcctga tgggcaagct
    14101 gccgggcatg gggcagatcc cggataatgt taagtcgcag atggacgata aagtgctggt
    14161 gcgtatggaa gccatcatca actcgatgac gatgaaagag cgcgctaagc cagaaatcat
    14221 caaaggttcg cgtaaacgcc gtattgctgc cggttgcggt atgcaggtgc aggacgttaa
    14281 ccgtcttctg aaacagttcg acgacatgca gcgcatgatg aagaaaatga agaagggcgg
    14341 aatggcgaag atgatgagaa gcatgaaggg tatgatgccc ccaggcttcc ctggtcgcta
    14401 atcggcctgg cttgtttgcc attttggcac cgggatgtgc tcgtcatcct cacgtactgc
    14461 gtgtacgctc cggtgactgc gcgcactccg gcaccaaact ggctgcaccg ccgacggcct
    14521 tttacagcag agttagcaac tgttgattgc aattcccgca aaaatgagta aaattttcgg
    14581 gcttttaata tgacaccgga ctccgttcct cgatggggtc cggttgtttt attcacacaa
    14641 gaggatgtta tggtaactat tcgtttagca cgtcacggcg ctaaaaagcg tccgttctac
    14701 caggttgttg tcgctgacag ccgtaatgca cgcaacggtc gcttcatcga gcgcgttggt
    14761 ttcttcaacc caatcgctag cgaaaaagaa gaaggcactc gcctggatct ggatcgcatc
    14821 gctcactggg ttggccaggg cgcaactatt tctgatcgcg ttgctgcgct gatcaaagaa
    14881 gtaaacaaag cagcttaatc tgtcacggtg gtcatgatga gcaaacaact caccgcgcaa
    14941 gcacctgttg atcccatcgt tttgggaaaa atgggttcgt cttacggtat tcgtgggtgg
    15001 ctcagagtgt tttcttccac cgaagacgcc gaaagcattt ttgactatca gccctggttt
    15061 atccagaagg cgggtcagtg gcagcaagtc cagctggaaa gctggaagca ccacaatcag
    15121 gacatgatca tcaagctgaa aggcgttgac gatcgtgatg cggcgaacct gctgacgaat
    15181 tgtgaaattg tcgtggattc atcgcagctg cctcagcttg aagagggtga ctactactgg
    15241 aaagacctga tgggctgcca ggtagtaacc actgaaggct acgatctcgg taaagtcgtc
    15301 gatatgatgg aaaccggatc taatgacgtt ctcgtcatta aggcaaacct gaaagatgcg
    15361 tttggtatca aggaacgtct cgtaccgttc ctcgatgggc aggttatcaa gaaagtcgat
    15421 ctcactactc gttcaatcga agtagattgg gatcctggtt tttaaaccac cggataaacg
    15481 gtaaaagacg gcgctatgtg gattggcata attagcctgt ttcctgaaat gttccgcgca
    15541 attaccgatt acggggtaac tggccgggca gttaaaaatg gcctgctgag catccagagt
    15601 tggagtcctc gcgacttcac gcatgaccgg caccgtaccg tggacgatcg tccttacggc
    15661 ggcggaccgg ggatgttaat gatggtgcaa cccttgcggg acgccattca tgcagcaaaa
    15721 gccgcggcgg gtgaaggcgc aaaggtgatt tatctgtcac cacagggacg caagcttgat
    15781 caagcgggcg tcagcgaact ggcaacgaat caaaaattga ttctggtgtg cggtcgctac
    15841 gaaggtatag atgagcgcgt gatccaaacc gaaattgacg aagaatggtc aatcggcgat
    15901 tacgttctca gtggtggtga gttaccagca atgacgctga ttgactccgt ttcccggttt
    15961 attccgggag tactgggaca tgaagcctcg gcaacggaag attcctttgc tgaaggattg
    16021 ctggattgcc cgcactatac gcggcctgag gtgttagaag ggatggaagt tccgccagtg
    16081 ttactgtcgg gcaaccatgc cgagatacgt cgctggcgtt tgaaacagtc gctgggccgt
    16141 acctggctta gaagacctga acttctggaa aacctggctc tgactgaaga gcaagcaagg
    16201 ttgctggcgg agttcaaaac ggaacacgca caacagcaac ataaacatga tgggatggcg
    16261 taagcccccg agatatcagt ttacccagga taagagatta aattatgagc aacattatta
    16321 agcaacttga acaagagcag atgaagcagg acgtaccttc cttccgtccg ggtgataccg
    16381 tggaagtgaa agtatgggtt gttgaaggtt ccaaaaaacg tctgcaggca ttcgagggcg
    16441 tggttatcgc tattcgtaac cgcggtctgc actctgcatt cactgttcgt aaaatttcca
    16501 acggcgaagg cgttgagcgt gtcttccaga ctcactctcc ggtagttgac agcatttctg
    16561 tcaaacgtcg tggtgctgtt cgtaaagcta aactgtacta cctgcgtgag cgtactggta
    16621 aggctgctcg tatcaaagag cgtcttaact aagatttcgc ttaagcgaca tcctgttaag
    16681 aagggctggc caattggctg gccctttttt tgtctgtttg ctcgtgtttt aaggggttgt
    16741 aatcaccact gcgacgcggc ggttctcggc gcggccctgg gcggtcttgt tactggctat
    16801 gggatatttt tttcctaaac cctgtgtggt gagattgctg cgtggaattt gtccacccat
    16861 agcccatgca tcggccacga cattcgcccg tttcaatgat aagccttcgt tgtaactgtc
    16921 ttcaccatag ttatcggtgt gtccatccat acgggcatgt gttagccctg tcgaggccaa
    16981 tttggctgcc atggtttgga tctgttgctg gctttccggg agcaatttgt agtcattttt
    17041 tgcgaacaga atggcatctg ataagcccag cgaccagtcg ccggcggatt cagtaaatcc
    17101 ataagattgc atagcggcga cttgctcagg agtaaacttt ccctgagggg actggcagcc
    17161 agtcagtatt agtgaggtga aaatcagggg tgctaccagg tgctttatca tatcgatata
    17221 tccttgttat ctcaccagct tttcggcacg ctggtgtttg gcctgataca tattgtgatc
    17281 ggcaagctct tgtaattttt ccgcagaggc gtgctcaatg gtcatcgcgt aaccaatgct
    17341 taatgtcatg gtggtctgat ggccattatg aagatcaaac gggagattaa agatttgtgt
    17401 cagtgctgag catatctgct gcacttcaga ttccgactgt acatcataga gcaccatagc
    17461 gaattcatcg ccgcccaggc ggtatgcttt atgtcgcagc ccgccaaatt cagctaaccg
    17521 ttttgcgatt tcaatcaaga ctctatcgcc cgtcgcatga ccccaggtat cattgatata
    17581 tttgaaatta tcgccatcaa gaaatagtaa cgccgacgtt tttcgggcat cggaattgtt
    17641 catcaacgta ttgatgccgc tacgaaacgc tgcgcggtta gccagcccgg ttaatgggtc
    17701 atgtagcgcg gtacgcagaa gctgcgcatt tttggcctgt aaacgaagct gccactcttc
    17761 catttcatcc agcagactgt tgaagtcgag agcgaagcgg tgaaactcag cgatacgttc
    17821 ttccgaaact cgtcgggaaa aattgcggtt ggaacgcaca tcatgtacga catcggtgat
    17881 atttttcagc gcttctacca ggccattgtg caaatggcgg gtgagggtaa ttgcgatgcc
    17941 tgatgccagc agaatacaac cggtcagtac ggcgagcgaa aaccagataa aatggctgat
    18001 tgaactgtcg cgagcggtta agcgtacttc gccaatggtt tcaccattgt gacgaatcgg
    18061 ctgaatgatg ggggcgggga agagccagtg gcttatgaaa ttgctgaaag tatcgcctgg
    18121 atccttacgg gtgtaatgcc aggacgccag aatattttgc tgcttatcac gtacttctgc
    18181 agttgaaaat tgcccttgct ggcccagcgc tgccagtgtt tcagttgctg cagggccatc
    18241 ggcaaaaacg acagctgctt ccagactgta agtcattgtt gctgctgtca gtgccaggtt
    18301 tttttgcgcg tactgtttca gggtcagcac cgaggtcacg gaaagcagca accagatcag
    18361 catcatagtg ataaatatgc tggtcatact gatgttgcgt aatgctcttt taaacgtggg
    18421 gcgcttatta agagaattat cgttatccat catttttctt ccgtgcgagc attaagacat
    18481 ccgggttgac ctttacaccg ctacgcgata aggcatccag gtttacggca aatctgacgt
    18541 cattattgtg gatgatcaga caaaaggcgc taccaataat gcactcggta ttttgttcgg
    18601 caattaataa taacgccttt gacggatatt gttcggttaa ttccatctgg aatgtcggtg
    18661 actcgttgcc aaaataaaaa ccattgcagc ctgaaatcat cgcctcttgt tgagtgtgaa
    18721 tgatgacagg cagataaggc aaagatgtcg cggcattctc ttgtagggcg gtactaaaac
    18781 gtgaagatga aaagatacat aactttggtg gccctgatag cgcaggccag cgggtgtaac
    18841 tgacaatacc ggaaaccatc atacgcacgt ttttcgcaac gtcagaaggc tcctgagcga
    18901 gtattggtgc tcccgtcagc aggagaatca gaagtaagaa cagtcggtga gaaaaacgca
    18961 ttcagggatc cccaccatct tcgctcgaaa tgcatcgata ttttttcaga atgctcatta
    19021 aaatatcatt ttttcatgcg atcgtcatca atgtgagaaa taaaacacaa tgagtaaaat
    19081 cttatgttta atttatttgt ggggttttaa ttaatttaat cgtattgtgc tggcattttc
    19141 atcagcccct ttttgccata cttcatgcag ttggctggca ttttcaacat tgttgcagct
    19201 ggcaggaaag cttttaccgg aaagtccccg ggcataagta aaatcggtct ggcaagtttt
    19261 cttttgtcct tcggcatatc ctttaaggta tagaccgcga tccgcctgtg aatcgctaaa
    19321 tgcatcgtca tcttttatgg cgctgcccga tatcgcatct tccataccaa catcatacca
    19381 gtcggtgctg gtcaaggttg gcgcgtgagt ataaggatca atctgacatc cgctaaccag
    19441 taaagccaac aagggggcga taaacttttt catcatttct ttctcctttt tcaaagcata
    19501 gcggattgtt ttcaaaggaa gtgtaaattt atctatacag aagtaagggt tgaaatcgcg
    19561 actaaattgc ctgtgtaaat aaaaatgtac gaaatatgga ttgaaaactt tactttatgt
    19621 gttatcgtta cgtcatcctc gctgaggatc aactatcgca aacgagcata aacaggatcg
    19681 ccatcatgca aaaagacgcg ctgaataacg tacatattac cgacgaacag gttttaatga
    19741 ctccggaaca actgaaggcc gcttttccat tgagcctgca acaagaagcc cagattgctg
    19801 actcgcgtaa aaccatttca gatattatcg ccgggcgcga tcctcgtctg ctggtagtat
    19861 gtggtccttg ttccattcat gatccggaaa ctgctctgga atatgctcgt cgatttaaag
    19921 cccttgccgc agaggtcagc gatagcctct atctggtaat gcgcgtctat tttgaaaaac
    19981 cccgtaccac tgtcggctgg aaagggttaa ttaacgatcc ccatatggat ggctcttttg
    20041 atgtagaagc cgggctgcag atcgcgcgta aattgctgct tgagctggtg aatatgggac
    20101 tgccactggc gacggaagcg ttagatccga atagcccgca atacctgggc gatctgttta
    20161 gctggtcagc aattggtgct cgtacaacgg aatcgcaaac tcaccgtgaa atggcctccg
    20221 ggctttccat gccggttggt tttaaaaacg gcaccgacgg cagtctggca acagcaatta
    20281 acgctatgcg cgccgccgcc cagccgcacc gttttgttgg cattaaccag gcagggcagg
    20341 ttgcgttgct acaaactcag gggaatccgg acgggcatgt catcctgcgc ggtggtaaag
    20401 cgccgaacta tagccctgcg gatgttgcgc aatgtgaaaa agagatggaa caggcgggac
    20461 tgcgcccgtc tctgatggta gattgcagcc acggtaattc caataaagat tatcgccgtc
    20521 agcctgcggt ggcagaatcc gtggttgctc aaatcaaaga tggcaatcgc tcaattattg
    20581 gtctgatgat cgaaagtaat atccacgagg gcaatcagtc ttccgagcaa ccgcgcagtg
    20641 aaatgaaata cggtgtatcc gtaaccgatg cctgcattag ctgggaaatg accgatgcct
    20701 tgctgcgtga aattcatcag gatctgaacg ggctgctgac ggctcgcgtg gcttaagagg
    20761 tttattatgg ttgctgaatt gaccgcatta cgcgatcaaa ttgatgaagt cgataaagcg
    20821 ctgctgaatt tattagctaa gcgtctggaa ctggttgctg aagtgggcga ggtgaaaagc
    20881 cgctttggac tgcctattta tgttccggag cgcgaggcat ctatgttggc ctcgcgtcgt
    20941 gcagaggcgg aagctctggg tgtaccgcca gatctgattg aggatgtttt gcgtcgggtg
    21001 atgcgtgaat cttactccag tgaaaacgac aaaggattta aaacactttg tccgtcactg
    21061 cgtccggtgg ttatcgtcgg cggtggcggt cagatgggac gcctgttcga gaagatgctg
    21121 accctctcgg gttatcaggt gcggattctg gagcaacatg actgggatcg agcggctgat
    21181 attgttgccg atgccggaat ggtgattgtt agtgtgccaa tccacgttac tgagcaagtt
    21241 atcggcaaat taccgccttt accgaaagat tgtattctgg ttgatctggc atcagtgaaa
    21301 aatggaccat tacaggccat gctggcggcg cacgatggcc cggtactggg gttacacccg
    21361 atgttcggcc cggacagcgg tagcctggca aagcaagttg tggtctggtg tgatggacgt
    21421 aagccggaag cataccaatg gtttctggag caaattcagg tctggggcgc tcggctgcat
    21481 cgtattagcg ctgtcgagca cgatcagaat atggcgttta ttcaggctct gcgccacttt
    21541 gctacttttg cttatgggct gcatctggca gaagaaaatg ttcagcttga gcaacttctg
    21601 gcgctctctt cgccgattta ccgccttgag ctggcgatgg tcgggcgact gtttgctcag
    21661 gatccgcagc tttatgccga cattattatg tcgtcagagc gtaatctggc gttaatcaaa
    21721 cgttactata agcgtttcgg cgaggcgatt gagttgctgg agcagggcga taagcaggcg
    21781 tttattgaca gtttccgcaa ggtggagcac tggttcggcg attacgcaca gcgttttcag
    21841 agtgaaagcc gcgtgttatt gcgtcaggcg aatgacaacc gccagtaata atccagtgcc
    21901 ggatgattca catcatccgg caccttttca tcaggttgga tcaacaggca ctacgttctc
    21961 acttgggtaa cagcccaata ccttcattga acgggtaatt tcccctaact ctttcaatgc
    22021 tttttgcatt tccgctgatt caagattggc ctgaatatcc agataaaaca tctcttccca
    22081 tggattaccg tgaatcgggc gtgattccag acgggtcata atcagattgt ggttgcgcag
    22141 caccagcaac gcttcaacca gcgcaccggc ttgttgcccg gtcgccatta acaacgtcgt
    22201 tttcgccgga acctggtcag acacgttaat ggctttacgt gccaacacca caaatcgggt
    22261 gaagttttgt tgctgattcg cttcaatacg ctccagtacc tgcaaaccgt acaaagtgcc
    22321 gccagcttcg cttcccaatg cagcaacatg cggtgatttt gcctgtgcaa ccttttccat
    22381 tgccgcagac gtactttcgg tatattcaat cttccagtga ggataacgat taaggaattt
    22441 gctgcattgc tggaatggct gcggatggct gtagaccgta ttaatggtgg ataaatcagt
    22501 cgtgccggag accaacaaac aatggtcgat agttaacgtc atctcgccaa caatcgacaa
    22561 gctggtatgt tgcagcagat cgtaaacgtc gtttatggca ccggagctgg
//
LOCUS       DAEVZA010000081        22363 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEVZA010000081 DAEVZA010000000
VERSION     DAEVZA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.081"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1053
                     /locus_tag="LAF92_003085"
     CDS             <1..1053
                     /locus_tag="LAF92_003085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Has polymerase, DNA-binding and 3'-5' exonuclease
                     activities. In Aeropyrum pernix this protein is sensitive
                     to aphidicolin and stable at 95#C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBI9792051.1"
                     /translation="DPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVTNIWHGR
                     DEAKRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHQIMRQTKALIE
                     AQGYDVIYGDTDSTFVWLKGAHSEEEAAKIGRVLVQHVNAWWAETLQKQRLTSALELE
                     YETHFCRFLMPTIRGADTGSKKRYAGLIQEGDKQRMVFKGLETVRTDWTPLAQQFQQE
                     LYLRIFRNEPYQEYVRETIDKLMAGELDARLVYRKRLRRPLSEYQRNVPPHVRAARLA
                     DEENQKRGRPLQYQNRGTIKYVWTTNGPEPLDYQRSPLDYEHYLTRQLQPVAEGILPF
                     IEDNFATLMTGQLGLF"
     gene            1218..4124
                     /gene="rapA"
                     /locus_tag="LAF92_003086"
     CDS             1218..4124
                     /gene="rapA"
                     /locus_tag="LAF92_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBI9792052.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQIMESLDQAGWRLDALRLIVVTHQ"
     gene            4136..4795
                     /gene="rluA"
                     /locus_tag="LAF92_003087"
     CDS             4136..4795
                     /gene="rluA"
                     /locus_tag="LAF92_003087"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBI9792053.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(4912..5727)
                     /gene="djlA"
                     /locus_tag="LAF92_003088"
     CDS             complement(4912..5727)
                     /gene="djlA"
                     /locus_tag="LAF92_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBI9792054.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            5982..8336
                     /gene="lptD"
                     /locus_tag="LAF92_003089"
     CDS             5982..8336
                     /gene="lptD"
                     /locus_tag="LAF92_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBI9792055.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            8389..9675
                     /gene="surA"
                     /locus_tag="LAF92_003090"
     CDS             8389..9675
                     /gene="surA"
                     /locus_tag="LAF92_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBI9792056.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATADIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            9675..10664
                     /gene="pdxA"
                     /locus_tag="LAF92_003091"
     CDS             9675..10664
                     /gene="pdxA"
                     /locus_tag="LAF92_003091"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBI9792057.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDIIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            10661..11482
                     /gene="rsmA"
                     /locus_tag="LAF92_003092"
     CDS             10661..11482
                     /gene="rsmA"
                     /locus_tag="LAF92_003092"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBI9792058.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            11485..11862
                     /gene="apaG"
                     /locus_tag="LAF92_003093"
     CDS             11485..11862
                     /gene="apaG"
                     /locus_tag="LAF92_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBI9792059.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            11869..12717
                     /gene="apaH"
                     /locus_tag="LAF92_003094"
     CDS             11869..12717
                     /gene="apaH"
                     /locus_tag="LAF92_003094"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBI9792060.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            12742..13341
                     /locus_tag="LAF92_003095"
     CDS             12742..13341
                     /locus_tag="LAF92_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HBI9792061.1"
                     /translation="MADYFEIRADYNQHTITVYQAYNDAIADVAVRDGRFGASFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            13377..13844
                     /locus_tag="LAF92_003096"
     CDS             13377..13844
                     /locus_tag="LAF92_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792062.1"
                     /translation="MRQINSGLNTREIILAMIDEADEPPFAEQRTWHRRRIAFLCDCI
                     SVAIDYGFFCSVSQQSIMNITRMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYVHK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADIIQLRCDV"
     gene            complement(13943..14122)
                     /locus_tag="LAF92_003097"
     CDS             complement(13943..14122)
                     /locus_tag="LAF92_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HBI9792063.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(14143..14622)
                     /gene="folA"
                     /locus_tag="LAF92_003098"
     CDS             complement(14143..14622)
                     /gene="folA"
                     /locus_tag="LAF92_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBI9792064.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(14815..16677)
                     /gene="kefC"
                     /locus_tag="LAF92_003099"
     CDS             complement(14815..16677)
                     /gene="kefC"
                     /locus_tag="LAF92_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBI9792065.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(16670..17200)
                     /gene="kefF"
                     /locus_tag="LAF92_003100"
     CDS             complement(16670..17200)
                     /gene="kefF"
                     /locus_tag="LAF92_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBI9792066.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(17308..18639)
                     /locus_tag="LAF92_003101"
     CDS             complement(17308..18639)
                     /locus_tag="LAF92_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9792067.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(18698..18985)
                     /gene="fixX"
                     /locus_tag="LAF92_003102"
     CDS             complement(18698..18985)
                     /gene="fixX"
                     /locus_tag="LAF92_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBI9792068.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(18982..20268)
                     /locus_tag="LAF92_003103"
     CDS             complement(18982..20268)
                     /locus_tag="LAF92_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI9792069.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(20319..21260)
                     /locus_tag="LAF92_003104"
     CDS             complement(20319..21260)
                     /locus_tag="LAF92_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBI9792070.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(21275..22045)
                     /locus_tag="LAF92_003105"
     CDS             complement(21275..22045)
                     /locus_tag="LAF92_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBI9792071.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         5549 a         6087 c         5700 g         5027 t
ORIGIN      
        1 gatcccgtcg ggctggttga aggcatggcg caacctgatc cagagcacag taccgaaggt
       61 tttctcgatg cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc
      121 tggcacgggc gcgatgaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag
      181 atcattatga atgcctttta tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg
      241 cgcctggcat cgtcgatcac catgcgtggt catcagatca tgcggcagac caaagcattg
      301 attgaagcgc agggctacga cgttatctac ggagataccg actcgacttt tgtctggcta
      361 aaaggtgcac attcggaaga agaggcggcg aaaatcggtc gtgtactggt gcagcacgtt
      421 aacgcctggt gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag
      481 tatgaaactc atttctgccg ttttctgatg ccaaccattc gcggagccga taccggcagt
      541 aaaaagcgct acgccgggct gattcaggag ggcgataaac agcggatggt gtttaaaggg
      601 ctggaaaccg tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctatac
      661 ctgcgcatct tccgcaacga gccatatcag gaatatgtac gcgaaaccat cgacaaactg
      721 atggcgggtg aactggatgc gcgactggtt taccgtaaac gccttcgccg tccgctgagc
      781 gagtatcagc gtaatgtgcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaac
      841 caaaagcgcg gtcgcccctt gcaatatcag aatcgcggca ccattaagta cgtatggacc
      901 accaacggcc cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg
      961 acccgccagc tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct
     1021 acacttatga ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca
     1081 ttttgatgcg tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac
     1141 ctgaataaca ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga
     1201 tatagagccg aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa
     1261 gcgaattggg acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc
     1321 catctactgg tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt
     1381 tcaaccctgg tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa
     1441 aagaagaaaa tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg
     1501 tagccctgcg tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc
     1561 tgtttgccgg gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt
     1621 attccagcga acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga
     1681 ttccgcatca gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc
     1741 tggctgacga agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac
     1801 tgctctctgg cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt
     1861 ggctggtaga aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt
     1921 atgccgaagc tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct
     1981 cgctggattt tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg
     2041 acctgctggt ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg
     2101 aatatcaggc cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga
     2161 ccccggaaca gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc
     2221 gtttccacga ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg
     2281 ccgttgccat gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg
     2341 agatgatcgg cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag
     2401 atgcccagag cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc
     2461 gcgtgctgtt ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca
     2521 ccattaaact gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg
     2581 gcgcacgtaa aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc
     2641 aggaatttga aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga
     2701 tgggctacct gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca
     2761 ctgcgctgca actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc
     2821 acgaaggtat gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca
     2881 ccggcgcaca ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg
     2941 ccagccacat ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg
     3001 gtcgtctgga tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga
     3061 aaaccgctca gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca
     3121 cctgcccgac cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg
     3181 cttcaccgga tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg
     3241 aagcgctgaa agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg
     3301 gtggcgaaaa agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc
     3361 tgatcgcctt cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg
     3421 acaacatgat cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt
     3481 cggaagatgg catcaccatc acctttgatc gtgaagtggc actggcgcgt gaagatgcac
     3541 agtttattac ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg
     3601 ataccggtag cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt
     3661 tggtggaact gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct
     3721 tcctgccacc gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc
     3781 aggtagagtt tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca
     3841 aactggttaa cgccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga
     3901 tcgagaaatc tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt
     3961 ctgccgagct gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg
     4021 aactgaccgc cattgagagc aaccgtcagc agataatgga aagcctggat caggcaggtt
     4081 ggcgtctgga tgccctgcgt ttgatcgttg tgacgcatca gtaacggagc tgaaaatggg
     4141 gatggaaaac tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca
     4201 cattatggtg gtcaacaagc cgagcggttt gttgtctgtg ccgggtcgtc tggaagagca
     4261 caaagacagc gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg
     4321 tctggatatg gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga
     4381 gttaaaacgc cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg
     4441 gcatccatcc cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg
     4501 cccgaaacag aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt
     4561 ggagtatgcg gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca
     4621 tcaattgcgt gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc
     4681 atcaccagaa gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat
     4741 tacccatccg gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc
     4801 acgctggcgc agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc
     4861 gattgcctga tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa
     4921 ccctttctgc tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc
     4981 catctccatc atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact
     5041 catcagctta cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag
     5101 cacattacag gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc
     5161 agattgctgc tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg
     5221 gtcaaactgg gcgcgggaga tccctaattc ttcagcaata acatacagca ccgcccgttc
     5281 attcgggtgc agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg
     5341 aattaagtca aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg
     5401 gtaattgtct gattttccca cccggaacgc attttgcgcc gcagtacggg aagcgccatg
     5461 aagattcatt cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc
     5521 tttggatttg gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg
     5581 ctcacgctgg ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc
     5641 aattaacagg cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac
     5701 gccaatgatt tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc
     5761 tatctcccac aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat
     5821 cgcctataat ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct
     5881 attcggacag gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt
     5941 cacgcgcaac gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca
     6001 ctctcctggc caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc
     6061 tcgcctcaca gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata
     6121 ccaatgactt acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg
     6181 ccgtgtttac tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag
     6241 tgcagctcca tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc
     6301 tcggtaatgt ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga
     6361 atctgaacac caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg
     6421 gtcgcggtaa agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata
     6481 acggtagctt tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa
     6541 ttattcatga ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc
     6601 cggtaccgat cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg
     6661 gtttcttgat cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat
     6721 attactggaa catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc
     6781 gtggcaacat catgtgggag aacgaattcc gctacctctc ccaagcgggc gctggcttga
     6841 tggaactgga ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca
     6901 gttcacgtcg ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt
     6961 tcaacgtcga ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt
     7021 acggttccag tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa
     7081 acttcaatgc caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta
     7141 gctactcggc agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg
     7201 atacgcgtat ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag
     7261 caacccgtgt tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca
     7321 tcaataccga agcgaagttg ctggcaaccc attatcagca aactaatctt gactggtata
     7381 actccagaaa cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag
     7441 ttgacggcaa aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa
     7501 cgctggaacc gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata
     7561 actacgactc atctctgctg caatctgact actctggcct gttccgggac cggacttacg
     7621 gcggtcttga ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat
     7681 atgatgatgc tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg
     7741 agtctcgcac tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg
     7801 tgtgggcagg cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc
     7861 agtacgatac acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg
     7921 atgaagaccg tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta
     7981 cgctgcctaa gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg
     8041 ctgtcgccag ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca
     8101 ccaatgcgaa caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg
     8161 caattcgcgt cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg
     8221 tatatgacaa cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc
     8281 tgggtacgca agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg
     8341 ttgatttacc acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg
     8401 aaaacgctgc ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta
     8461 gtcgataaag tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga
     8521 ttaatgcagt cggtaaaact gaacgctgct caggcaaggc agcaacttcc tgatgacgcg
     8581 acgctgcgcc accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg
     8641 cagaaaatgg gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg
     8701 aaacagaaca acatgacgct ggatcagatg cgcagccgtc tggcttacga tggactgaac
     8761 tacaacacct atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac
     8821 gaggtgcgtc gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg
     8881 ggtaaccaaa acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg
     8941 gaaaacccga cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat
     9001 caggcgcgta acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag
     9061 gcgctgaacg gcggccagat gggctggggc cgtattcagg agttacccgg gatcttcgcc
     9121 caggcattaa gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc
     9181 ttccatattc tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa
     9241 gttcatgctc gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt
     9301 gtgaaactgg aacagattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca
     9361 gcgaaagagt tctcgcagga tccaggttct gctaaccagg gcggcgatct cggttgggca
     9421 acagcggaca tttttgatcc ggctttccgt gacgccctga cccgcctgaa caaaggtcaa
     9481 atgagtgcac cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt
     9541 aatgtcgata aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt
     9601 aagttctcgg aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa
     9661 atcctgagca actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg
     9721 ggattggccc ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg
     9781 tttgtgccga tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc
     9841 tccgcactta ttcccccaac tctcctgcac aaccgcaaac tgcgggcaca ttaacgctac
     9901 ttcctgtcgc gctacgtgaa tctgtcactg cggggcagtt agcggttgaa aatgggcatt
     9961 atgtggtgga aacgctggcg cgagcgtgcg atggctgtct gaacggtgaa tttgctgcgc
    10021 tgatcacagg ccccgtgcat aaaggcgtca ttaacgacgc aggcattccg tttaccggtc
    10081 ataccgagtt tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg
    10141 aagaacttcg cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta
    10201 tcacccctgc acttttgcac gaagtgattg ctattttgca tcacgatttg cggacaaaat
    10261 ttggtattgc cgaaccgcgc attctggtct gcggactgaa tccgcacgcg ggcgaaggcg
    10321 gtcatatggg tacggaagag atagacatca ttattccggt gctcgacgag ctgcgggcgc
    10381 aggggatgaa actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg
    10441 ataacgccga cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc
    10501 agggcttcgg gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg
    10561 accacggcac cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta
    10621 cggcgcttaa tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca
    10681 gggccactta gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga
    10741 cagcattgtt tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg
    10801 tctggcggca ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact
    10861 tgaccgcgat ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat
    10921 ttatcagcag gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc
    10981 gctgcgtgtt ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt
    11041 tagctatact gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg
    11101 tctggttgca ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta
    11161 ttgcaacgtg atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt
    11221 ggattccgcc gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt
    11281 tcgtgtgttg agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa
    11341 cagcctcggc aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat
    11401 gcgagcggaa aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggaaaa
    11461 cgcgcctttg caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa
    11521 agcgtctaca ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc
    11581 gtaaccatac gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc
    11641 accaatggca atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca
    11701 cttatcgcgc ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg
    11761 ggcaccatgc agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac
    11821 attcccgtat tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac
    11881 cttattggcg acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa
    11941 tttacccctg ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt
    12001 tcgctggatg ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc
    12061 aatcacgatc tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat
    12121 cgcctgacac cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc
    12181 cagcctttgc tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg
    12241 ccgcagtggg atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg
    12301 agtgactcct atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca
    12361 ccggaattgc gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt
    12421 ttttgctttc caaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct
    12481 gccccactga aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc
    12541 tttggtcact gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat
    12601 accggctgct gctggggtgg gtcattaacc tgcctgcgct gggaagataa gcagtatttt
    12661 gtccagccgt cgaaccggca taaggatttg ggcgagggag aggcggtcgc gtcttaacga
    12721 attaccattc agggaggaat aatggctgac tattttgaaa tacgtgctga ttacaatcag
    12781 cacaccatca cggtttatca ggcttacaat gatgcaatcg ctgacgtcgc cgtacgtgac
    12841 gggcgatttg gcgcatcttt ttcattcaac cgcatgacct ggattaaacc ctcttttatg
    12901 tggatgatgg agcgtagcaa ctggggactg aaaaaagatc aacaacacat tctcgcaatc
    12961 cgaatcaaac gcaccttctt tgatacattg ctggaacaag cggtgctaac cacgccggaa
    13021 gcccatgtgt atccacatgc aggcatttgg gaaacgttat ttgcacaagc aaatgtgtat
    13081 gttcagtggg atcccgaacg cagtataaat ggtaaaaaac tggagcaccg atcattacag
    13141 cttggtattt cccgtaactt gatcagtcaa tttaacgaag acgccattgt tgccattgat
    13201 gatctgactc cgctggtgcg aaagtgtcat aaccttttga taaacggtaa aacaacacag
    13261 gcgaaatcct tcctgccgcc agaaaaaatt taccctgtta gtgcagctgc gagaaaggct
    13321 ttgggaatga aaaattcctg agacaaatac gcgcctatta agcggaaaat atctaagtgc
    13381 gacaaatcaa cagcgggctt aataccagag aaattattct ggcaatgatt gacgaagcag
    13441 atgaaccccc atttgctgag cagcggacct ggcatcgcag gcgaatcgct tttctatgtg
    13501 attgcatcag cgttgctatc gactacggct tcttctgttc tgtctctcag cagtcaataa
    13561 tgaacataac caggatggct gaagatgtat ggataacaga aagtaaaacg acccaactca
    13621 ttgatatttg gcaacaactt aagcagcttt atccgccaga aactattccc tacgtccata
    13681 agttacccac taaggaatta cttgaactgc tactttctag tcctgatgat cttcgaataa
    13741 ttgaaattcc tgacatcatc ggtgatgttt ttgatgacca aacctggatt cggttattca
    13801 aaaaacactt tgctgatatt atccaactac gatgtgatgt gtaacaatcc aaaatatatc
    13861 gccagacgat agaccaaaaa gaaagtattt agcagatact caacattctg acttctgtac
    13921 agaaatagta gagtttaaaa acttaaaact tccggtactc atcagagaaa catcctgttt
    13981 cttctgcaaa ttcatttaat gccgagatgg catcctcgtt tacttcattc cactgtagag
    14041 tggcttgaag gtttagacct acgcaatgag cgcgactcac aaaatcgcgc tcactggtaa
    14101 aatcaaacgt cttatcgttc aatttgtagt cctggcaaaa aattaccgcc gctccagaat
    14161 ctcaaagcaa tagctatgag agttctgcgc atcggcgtcg tggaattcgc tgaataccga
    14221 ttcccagtca tccggctcgt aatccgggaa atgggtgtcg ccttccactt ctgcgtcgat
    14281 atgcgtcaga tacagttttt gcgcttttgg caggaactgc tcataaacgc gaccaccgcc
    14341 aatcaccatg atttctggta cgtcaccaca cgccgcgatg gcttcatcca ccgacttcac
    14401 ccacgttacg cgatcgtccg tacccggttg actgctgagg ataatatttt tgcgtcctgg
    14461 cagcggacga ccgatggatt cccaggtatg gcggcccata attacgggtt tatttaaggt
    14521 gttgcgttta aaccaggcga gatcggcagg caggttccac ggcatggcgt tttccatgcc
    14581 gataacacga tctaccgcta acgccgcaat cagactaatc attgatttat cctggatata
    14641 aaaaattgtc gccactatac gtaaagcata aaccgtcgtc gactggcgca aggatgatgt
    14701 tgagtaaaat tttacgttct gctggcgagt ccacgctctc ccactggact cgccacatta
    14761 caatgaaaca aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattagga
    14821 tgagggtttc gtttccggtt catccgccat gttgccggta tgtttacctt cttcggttcc
    14881 ctgccagcca tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtaa tgatctcact
    14941 taacatcgcg ctggtgcgtt tatagaccgc cgcgcgggct ttggtatcat tctcaaccat
    15001 tgccatctct tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc
    15061 ttcatacggc cccagaccta aactttccag tgccagacgc ccggttttca gcgcaccttc
    15121 gaaggtttca cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtggtcgac
    15181 atcgcgggcg cgggcaataa tctgcaaatg cgggaaatgt tctttcacca tctctgtcag
    15241 ttgcaggttg gtttgcggat cgtcgatggc gttaatcagc acttccgctt tcgccgctcc
    15301 ggcagattcc agtaaatcca tccgcgtggc atcgccataa aacactttca taccaaattt
    15361 acgcaaggtt tcgatatggt ccggatcgtg atcgagtacc accattttca ccccgctgga
    15421 gagcagtaaa cgtccggtaa tctgcccaaa acgaccgaat ccggcgataa tcacgcgcgg
    15481 ctgttcttcg tcgatctcat cggcttcacg cgcttcctca gtagaagatt gctcaaggcg
    15541 attgaggatc accagcagaa tcggcgttgc tgccatcgac agcgccaccg ccagggtcag
    15601 cgatttcgcc cactccggct ccagcacatt cgccatctgc gccgcgccaa ataccacaaa
    15661 ggcaaactca ctgccctgcc ctaacaacac cgcaaaccaa cgacgctgtt tatttggcac
    15721 ttgcaacggt cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag
    15781 caaaatgaca atgcgcaatg ggttttcaag cagcgtgcca aagtctatcg acatgccaac
    15841 accgatgaaa aacagcccca acagcaaacc tttaaatggt tcgatatcgc tctccagcgc
    15901 atgacggtat tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga
    15961 caagccgacc tcttccagca gcaaaccaaa gccaaacacg aggaataacg ccacggcact
    16021 aaacacttcc cgcaagccag agcgggctac aaaacgcagc gccggacgcg tgacatagcg
    16081 ccccagcaat accaccagca ccagcgcacc cgccactttt aacgccgaga gagcaaatgc
    16141 gcccatcgtc gtcgaggcac tgctcgttgc cagtagcgga atcatcgcca ccagcgggat
    16201 cgccgcgata tcctggaaca gcagcaccgc aaaggcactg cgacccattt gcgtcaccat
    16261 cagattgcgt tcattcatcg cctgcatggc aatcgccgta gaggagagcg ccagcgtcat
    16321 gccgatcaat tccgcgacct gccagcgcag cccaagtaac atgcagaaca gccccagcag
    16381 gccgccgcaa atcaccatct gcaatgcgcc accgccgaac actgccgcac gcagcttcca
    16441 cagcctttgt ggatcgagtt cgaggccgat aataaacagc atcagcacca cgccaatctc
    16501 ggcaaagtgc agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat
    16561 gcagccggcg atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac
    16621 aatcagcgct gccgaaccga gataaatcag cgcctgaatc agcgtatggc tatccatgat
    16681 gggcctcctg ccattccagc agacgttgct tatagtgacg cgcctgccct tcgagggttt
    16741 cgtcgtcaca aataaaggtg cagtgcatgg caaacggtgg cagccagttc agcccgcagt
    16801 agattgccgt cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt
    16861 caaaatggct ttccccgccg ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg
    16921 ccgtgccgcc gtgaccgtaa gcccagccgt gcgagaaaac tttatcgatc caaagtttga
    16981 ggagcggtgg aatgctgtac cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag
    17041 acagcgcctc ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag
    17101 agcgaatttc gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg
    17161 aatgatgcgg atacggatgc gcataaatta taagaatcat aggtagcccg cgtgattacg
    17221 cctttgtttt cccacaaagt gtagtcatat attctcgcgg cttatagtca ataatattga
    17281 ttcagaaaag agagaaatct gaacaattta ccccattctc tgcccgcgga tcgtcatatt
    17341 gctggtttgc gccagtgaca tccctcgagt ctccggggca aacgctacgg aaatcaacaa
    17401 gccaaacagc gagatacccg cccccattag catcgtgtta ctgataccgt aattattgat
    17461 aaagatcggc agtgcccagg tcgaaacaat ggtgccaata cggcttaagg acataatcac
    17521 gcccacggca gaggcgcgga tatctgtcgg gaagagttca ttaggataga gccactgcaa
    17581 attacccggc ccgccagaga aaaaggcata caccgcaaag gccatcacta ccagccagat
    17641 ccccatatcc gggatcagcc ccaaaaccgc cagcgccagc gtcatcatgg caaagctgcc
    17701 aatcaacaat ggacgccgtc cggcagtgtt taaccacagc atcggcggaa tacagccgag
    17761 cataaagaac aggctaatca ccacgttccc cagcgccgcg tttttgccaa cacccaatcc
    17821 caacaaacca acgatttgcg ggccaaaggt gtaaatggcg aacattggga tcacctggca
    17881 ggtccagatg gcggcaacaa acagaacaaa aggaaaatgg cggcgattaa acagatcgcg
    17941 aaaacgggtt tgctgcggtt gctcttcatc gaaagccacc ggttcgccaa acagttttat
    18001 catcatctct tcgcactctt ttactcgccc tttgcgtaat aaccagcggg gagattcagg
    18061 cagttcgaat cgaccaatca aaatcaacaa acaggggatc gccgcgctac ccagcatcca
    18121 gcgccagccg ccttccacat cataaagcca gtagccgacc agatcggcgc aggtcgcgcc
    18181 gacataccac atggcggcga taaagctgat ggaaaacgcc cgctgacggg tactggagaa
    18241 ctcggtgatc attgaggtgg cgatgggata atctgcaccg atgacaatgc cgataagtac
    18301 ccgcatcacc aacagttcga cgggggatga aacaaacatc gtcgccaccg atatcacgcc
    18361 gatggcgatg atatcaatga ggaacatttt gcgccgtccg actttatcgg aaatatagcc
    18421 aaacagcgat gtgccaacga acagcccggc gagcgttccc gcgcccaaca agccaatcca
    18481 gtcagcgtcc agttttagcg ccggagtcag ttgctccagc gccacgccaa tcattaccag
    18541 tacataacca tccagaaacg gaccaccgct tccccacagc aaaatgcggc ggtgaattga
    18601 ggagaatttg agatcgtcaa agtttctgga cggttgcatg ggttattcct gtttttttgc
    18661 aatgagagtc agtacggcgt tacgtatcaa accaacatca gccgtaacgg aactccacac
    18721 caaaggtgcc gcgcgggtat tcccactgtt ccagcgccga ccccagcccc agaatgcgac
    18781 aggtgccgca ctccagacat ccggcgtaat cgaagcgcac actgccgtca tcctgcttct
    18841 tgtacagacc tgcggggcac gctttcacca gcagctccag ggcctgttta tcggcatcgg
    18901 ccttcacaac aatgtgcgga tgctcttcat cgacattgaa tttattgacg cccagtttga
    18961 cgtccacatt gacgggagaa gtcataaaac ggtcactcct ttcatgccat ccttgatcag
    19021 attgatgaag cccactttct tgccgtggcg gaggattttc ttgcgcatca gttccggcgc
    19081 actgccgtca atggtgaaca ggtcacgcgc cacgcccacc gccagttccg ggtagccgct
    19141 aaacatgcgt gggttatcaa ggaaggccgg tagtttctgg tacatacgca tatcgcgcag
    19201 cgggccactc tcaagatgct gacgatattc cgccagtttt tgcttactga aatcgtcgct
    19261 tttcatcgct gaaagcacgg tttttgctgc ggcttccccg gcggcaatcg ccagatccat
    19321 accgcgaatg gtaaaaccga ggttcataca cattccggcg gcatcaccgg caatcaatac
    19381 gccgtcacca accaactccg gcagcatgtt gatgcctgct tccggcacta cgtgagcgga
    19441 atattccacc agcttgccac ccgcgatcag cggtgcaacg gccggatgtt gtttgaaatc
    19501 ttccagcatt tgcggcaccg atttttttgc gtcatgcaga tgatgcagac cacaaaccag
    19561 ccccagcgac agggtatttt cattggtata aaggaagccg ccgcccatca ggccatcggt
    19621 gggtgatccc gcaaacagac aagccgcgcc ctgattaccc tgcaactgaa aacggtcttc
    19681 gattaccgac ttcggtaact cgatcagttc cttcacgcca accgccacat ccgtcggttt
    19741 aacgcgtttt gccatcccca gcttttcggc aaggatggag ttcactccat cagcaaggat
    19801 caccgttttc gcttcaatca catcgccatc ggcttctaca ccgacgactt tgccatcgcg
    19861 ctgtacgagg ttatcgacgc ggatcccggt aattaactgc gcgcccgctt cttcggcctg
    19921 ctccatcagc caggcatcaa atttactgcg caaaacggag taagaacgct gggatggcga
    19981 ggtttcgtca ccattgcagt agtccatagt catcgctgac ttttccgtca taaacgcgag
    20041 tttttcatgg gtgatcaggc gttctacggg ggcggagtcg gcgaaaccag gaataatgtg
    20101 ttccaggctg tgggcataga gacgcccgcc ggtgacgttc ttggcacctg cggaattgcc
    20161 acgctcgata actaacactt gcgccccttc gcgggcgagc accagtgcgg caaccgaacc
    20221 ggcaagccca gcaccgacga tgatggcgtc aaagatatct tcggacataa gagctccctg
    20281 tcagcggcag ggccaaaatg cagccctgcc agagtggatc aacgcgctaa agctgcggtc
    20341 agcgccggaa ggatcttcac ggcgtcacca acaatgccgt aatccgcgta ctggaagatc
    20401 ggcgcatttt tatctttatt gatggcgaaa atggtttgcg atgcattagc accaaccatg
    20461 tgctggatct gcccggagat ccccaccgcc aggtacagtt caggtttcag catcaggttg
    20521 gagataccga catagcgttc gtgctccatc catttttcgt tttccgccac cggacgagaa
    20581 caggccaact ccgcacctat cgccttgcaa agctgttctg ccagcgcaat gttctctttg
    20641 ctgccaatac cgcgaccgac gctgaccacc agacgggctt tgtcgagatc gacgctgttg
    20701 ctctggcgcg cctgggttgc cgtgcgggta atcgccacag ccggagcctg ccactccacg
    20761 gtgtgcgttt cgccagtgcg tgacgcgtct ggctgagccg catcgaacgt gccgctgctg
    20821 atggtcagta ccgcatacgg cgtggcaatg cgttcttcgc caatcgccag accaccgtaa
    20881 accatgtgtt tcactgtcgc tttaccgtcc tgtacgctga cggtgctggc atcgttagac
    20941 accgccgctt taaggcgata acccagtttt gccgccagta atttgccgcg acgggtgttt
    21001 ggcagcagca ccaggccgtc tgcgccgtgc tggcgaatag tgtcagccat gacaccggcg
    21061 taatcttcga tcatccgatc gtccggtttg ccgtttaatt tccagacatg attagcgccg
    21121 agctggattg cctgtgcgcc gtcggcatca ttgaggacaa aggtgttgat ttgattagct
    21181 aaagcctgcg caccgttcat cagttccggc agacgagaag gggtatcgct gaatacccag
    21241 acttgagaaa acgtgttcat agcatcccct gtaattaaat gactttgcga agattttcag
    21301 caaatgcggc gatctgttct tcgccgtcgc cttcaatcac gatgcgctga cgttcgcgct
    21361 gtttcggcgc agcaacctgt tgttcagacc aggcgtccac ggcgttaaaa ccgatatccg
    21421 ccgccgacca tacctggact ggctttttcg ccgcgccgag aatggctttc atcgaaggaa
    21481 tttgtgggga gttgatatca gtggaaacag caacaaccgc aggcagcgga atgcttaagg
    21541 tttcggtttc atcttccagt tcgcgctcaa cggtgagggt atctgccgtc agggagataa
    21601 ttttgctgac gccgttaact gccggaatat tgaggatttc gcccaccagc agaccaacct
    21661 gctgggcata aaggtcggaa gaaccatcgc cacagaggat caaatcaaag cctgctttct
    21721 gggcggctgc agccagtgcg ctcgccgttt gttgcggcag tgcctgctcg aactggtcat
    21781 caatcaccac aatcagttca tccgggccgc gcgatagcac atctttacgc cctttggcgt
    21841 tggtcagggc tttaccgccc acacttaagg ccgtcacctg cgcctctgct gcctgttgct
    21901 ttagctggca agccgcttca atagcgttca gatcgtattg gcttattttg gcatcggctt
    21961 tgctgaagtc taatgaacca tcagcattat tgaccgcaat atcctgttca tcaggcacgc
    22021 acttatagca agtaataatc ttcattgcat ctccagaaat catgaaggaa acgtcgttca
    22081 tatcggtggt gttaattaca gatatcacgc cctttaattt ggaaataaaa ctttaatcac
    22141 caatattgaa aatgtcacac gcagataaat ccaactttca atattgttaa gttcctcacc
    22201 aatattgaaa acatggcgta gaaaaaagaa attttcaata ttgctttatg gatcaccaat
    22261 attgaaagtc agatgctatt tttttactca caacacaaca taacaaactg attaataatc
    22321 aaaattaacg agaaaatcgc cctctattca cccgaaacaa aaa
//
LOCUS       DAEVZA010000082        22319 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEVZA010000082 DAEVZA010000000
VERSION     DAEVZA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.082"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..941)
                     /gene="araH"
                     /locus_tag="LAF92_003106"
     CDS             complement(<1..941)
                     /gene="araH"
                     /locus_tag="LAF92_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBI9792072.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAA"
     gene            complement(956..2470)
                     /gene="araG"
                     /locus_tag="LAF92_003107"
     CDS             complement(956..2470)
                     /gene="araG"
                     /locus_tag="LAF92_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBI9792073.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2540..3529)
                     /gene="araF"
                     /locus_tag="LAF92_003108"
     CDS             complement(2540..3529)
                     /gene="araF"
                     /locus_tag="LAF92_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HBI9792074.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKCTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            4326..4829
                     /locus_tag="LAF92_003109"
     CDS             4326..4829
                     /locus_tag="LAF92_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBI9792075.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4908..5159)
                     /locus_tag="LAF92_003110"
     CDS             complement(4908..5159)
                     /locus_tag="LAF92_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBI9792076.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(5274..5360)
                     /gene="azuC"
                     /locus_tag="LAF92_003111"
     CDS             complement(5274..5360)
                     /gene="azuC"
                     /locus_tag="LAF92_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBI9792077.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5623..5946
                     /locus_tag="LAF92_003112"
     CDS             5623..5946
                     /locus_tag="LAF92_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBI9792078.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            6117..6614
                     /gene="ftnA"
                     /locus_tag="LAF92_003113"
     CDS             6117..6614
                     /gene="ftnA"
                     /locus_tag="LAF92_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBI9792079.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6652..6891)
                     /locus_tag="LAF92_003114"
     CDS             complement(6652..6891)
                     /locus_tag="LAF92_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBI9792080.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            7082..8293
                     /gene="tyrP"
                     /locus_tag="LAF92_003115"
     CDS             7082..8293
                     /gene="tyrP"
                     /locus_tag="LAF92_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBI9792081.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(8355..9020)
                     /gene="yecA"
                     /locus_tag="LAF92_003116"
     CDS             complement(8355..9020)
                     /gene="yecA"
                     /locus_tag="LAF92_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBI9792082.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(9216..9302)
                     /locus_tag="LAF92_003117"
     tRNA            complement(9216..9302)
                     /locus_tag="LAF92_003117"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9266..9268),aa:Leu,seq:taa)
     gene            complement(9315..9388)
                     /locus_tag="LAF92_003118"
     tRNA            complement(9315..9388)
                     /locus_tag="LAF92_003118"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9354..9356),aa:Cys,seq:gca)
     gene            complement(9442..9517)
                     /locus_tag="LAF92_003119"
     tRNA            complement(9442..9517)
                     /locus_tag="LAF92_003119"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9482..9484),aa:Gly,seq:gcc)
     gene            complement(9669..10217)
                     /gene="pgsA"
                     /locus_tag="LAF92_003120"
     CDS             complement(9669..10217)
                     /gene="pgsA"
                     /locus_tag="LAF92_003120"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBI9792083.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLEQ"
     gene            complement(10274..12106)
                     /gene="uvrC"
                     /locus_tag="LAF92_003121"
     CDS             complement(10274..12106)
                     /gene="uvrC"
                     /locus_tag="LAF92_003121"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBI9792084.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(12103..12759)
                     /gene="uvrY"
                     /locus_tag="LAF92_003122"
     CDS             complement(12103..12759)
                     /gene="uvrY"
                     /locus_tag="LAF92_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBI9792085.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            13055..13231
                     /locus_tag="LAF92_003123"
     CDS             13055..13231
                     /locus_tag="LAF92_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792086.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            13218..13442
                     /locus_tag="LAF92_003124"
     CDS             13218..13442
                     /locus_tag="LAF92_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBI9792087.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(13509..14231)
                     /gene="sdiA"
                     /locus_tag="LAF92_003125"
     CDS             complement(13509..14231)
                     /gene="sdiA"
                     /locus_tag="LAF92_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBI9792088.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(14461..15213)
                     /gene="yecC"
                     /locus_tag="LAF92_003126"
     CDS             complement(14461..15213)
                     /gene="yecC"
                     /locus_tag="LAF92_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBI9792089.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MHPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(15210..15878)
                     /gene="tcyL"
                     /locus_tag="LAF92_003127"
     CDS             complement(15210..15878)
                     /gene="tcyL"
                     /locus_tag="LAF92_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBI9792090.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(15893..16879)
                     /gene="dcyD"
                     /locus_tag="LAF92_003128"
     CDS             complement(15893..16879)
                     /gene="dcyD"
                     /locus_tag="LAF92_003128"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBI9792091.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(16984..17784)
                     /gene="tcyJ"
                     /locus_tag="LAF92_003129"
     CDS             complement(16984..17784)
                     /gene="tcyJ"
                     /locus_tag="LAF92_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI9792092.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVETSLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKVVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(17872..18423)
                     /gene="fliZ"
                     /locus_tag="LAF92_003130"
     CDS             complement(17872..18423)
                     /gene="fliZ"
                     /locus_tag="LAF92_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBI9792093.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(18469..19188)
                     /gene="fliA"
                     /locus_tag="LAF92_003131"
     CDS             complement(18469..19188)
                     /gene="fliA"
                     /locus_tag="LAF92_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBI9792094.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(19508..20770)
                     /locus_tag="LAF92_003132"
     CDS             complement(19508..20770)
                     /locus_tag="LAF92_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HBI9792095.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSESDLSSIQDEIKSRLEEIDRVSSQTQFNGVNVLAKDGKMNIQVGANDGQTITIDL
                     KKIDSSTLNLSSFDATNLGTSVKDGATINKQVAVGAGDFKDKASGSLGTLKLVEKDGK
                     YYVNDTTNSKYYDAEVDTSKGEINFNSTNESGTTPTAATEVTTVGRDVKLDASALKAN
                     QSLVVYKDKSGNDAYIIQTKDVTTNQSTFNAANISDAGVLSIGASTTAPSNLTADPLK
                     ALDDAIASVDKFRSSLGAVQNRLDSAIANLNNTTTNLSEAQSRIQDADYATEVSNMSK
                     AQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            21036..>22319
                     /gene="fliD"
                     /locus_tag="LAF92_003133"
     CDS             21036..>22319
                     /gene="fliD"
                     /locus_tag="LAF92_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBI9792096.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNTMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDR"
BASE COUNT         5642 a         5773 c         5176 g         5728 t
ORIGIN      
        1 gctgccagca ggattaagcc gcgaaccacg tactgcgcga aaggagaaat attaagcagg
       61 ttcatggcgt tttccacggt gcctaaaatt aagatacccg ccaccacata tgagattttt
      121 ccgatgccac ctttcagaga aacgccacct aaaacgcagg cggagataac aataagctca
      181 taaccaatcg acgtcattgg ctgcccacta gtcatacgtg aagccagaat aattccggct
      241 atcgcagata ccaggccaga gagaacaaag ataataattt tggtgcgaac aaccggtaca
      301 cccgccagac gcgcagcctc ttcgttcccg ccaatcgcca gggtgttacg accaaaggtg
      361 gttttattca gcaacaaacc aaagataatc agacacgcga cggtgagcca gattggcgca
      421 ggcagaccga accagttagc gtaaccaagg gcaaagaagc tttcatcttc gataccgacc
      481 gctttaccgt ctgaaatgat gtacgccaga cctcgaacaa tctgcatcgt tgccagtgtt
      541 gtgatcagag catttatttt cagtttggcg ataacaaagc cattgaccag gccacagaga
      601 atgccaagca gcaaccccgc tgccacgcca atccacaggc tttcagtcag gttgataacc
      661 accgccgtgg tgacacccgc acaggcaatt acggaggcga cagaaaggtc aaagtcaccg
      721 gaagcaaggc agaacaacat gccacacgcc accatccccg acatggaaat tgccaggccc
      781 aaccctttca tattaatgaa ggtggcaaaa tttgggacaa aaatggcaca ggcgataaag
      841 agcaccgcaa acaccaccag catgccgtac tgatcccaga tacgcccgaa gctgaatgac
      901 gacttaggtg cgccagaccc agatgtagaa acagaagaca tcatactctc cttactcagg
      961 caactgcctg gctgacttta ggcatcgcaa ggctcagtgc ctgacgctca tctgcctgct
     1021 cgtgtaacaa ttcaccggcg atttcacctt cccgcatcac cacaatccgg tcggcaacgc
     1081 cgaggacttc aggtaagtcg ctggaggcaa acagcaccgc cacaccctgc gccgccagcg
     1141 cataaatcac gttgtaaatt tcatgcttag caccaacatc aatgccgcgc gtaggttcat
     1201 ccagcaaaat gaccttcatc tcttccgata accagcggcc cagaatggct ttttgctgat
     1261 ttccgcctga gagattcatg atcagttgct cagcgcccgg cgttttgatg ttgagcgaac
     1321 gaatgtggtg atcggcattg ttttcttccc aaccgttgtt gattacacaa ccgccgagca
     1381 catgtttacg tctggcactg atgttgatat tgtcgcgaac ggagtgcacg ggaataatgc
     1441 cttccgcttt gcgatcttcc gggcagagca tcatgcctgc ggcaatggcg tggctcggtt
     1501 tacgaatatc gatcggctgt tggtcgatat aaacctgacc ggcggtgatt tgcgtcccgc
     1561 caaacaagcc tttcattaat tcgctacgcc ccgcccctac cagaccaaac agcccaacaa
     1621 tttcaccact gcgaaccgcc agacttattg gcgtacgcac gcctggtgct ttcacagcat
     1681 caagacgcag gcgctcctcg ccataactac gaggttgcca gccgtagata tcgccaatgt
     1741 cgcgcccgac catcgcctgc accagcgcgt cgtggtcaac ctgctgcata tcggtaaagg
     1801 ttttgacata acgtccatct ttaaagacag tgatggcatc gctgagggca aatatttctt
     1861 ccatacggtg agaaacgtat aagatcaccc gcccctcttt tcgcagttca cgaataacgc
     1921 ggaaaagatt gtcgatttcg cgggcagaaa gggagctggt tggctcatca aaggcgataa
     1981 ttttagcgtt acgcgccagc gccttggcga tttcaaccat ctgccactga ccaatggaga
     2041 gatatttcag cggcgtgtcc gggtcaatat ccataccaag atgtttaagt tgtaaacccg
     2101 cctcataatt cagcaatgag cgattcacaa tgccgccttt atgcggcagc tggccgagat
     2161 agatgttttc cgcgacggtc atttccggca cgagatgcag ttcctggtaa ataatcgcca
     2221 cacccgcatt aagtgctgcg gtcgtgtcgg aaaaggacat ttcctgccca ttaatcacta
     2281 cagaacccgt ggttggcgca tagttgccgc tgaggatttt taagagagtt gattttcctg
     2341 cgccattttc acccatcaac gcatgaacct gaccggcata gcagtcaaaa ctaatatccg
     2401 tcagcgcctt aacgccggga aacgttttac cgatgccgcg aaatgagaga tacggggtag
     2461 actgttgcat aacgtctccg tgaatcactg gtcgttcaca ccccctctgc atcatgcaga
     2521 ggggaatttt tccggcaaat tacttaccgc ctaaaccttt tttctccagt tcttctttaa
     2581 agttgtcacg cgtgatcagc accacgtcgg taacttcggt acattttggc ggttcaacgt
     2641 cttttgctac ccagttgtaa agcatttcgc tggatttata gccatgtacg tccgggcttg
     2701 gcagcaggga accgtagaag ccggttgcct gtgctttaga cagttcgctc accgcatcca
     2761 caccgttaat gccaatgccg atgatatcgg cagctttaaa gccctgacct tccgtcgcgc
     2821 gtacgccgcc cagcacggtg ctgtcgttca taccgacgat cagccaatgt ttaacttccg
     2881 gatgttgaac cagcattgag ttggcagcgt caaatgcccc cgggatgtcg ttagatttgg
     2941 taggtacctg ataaatttgt ttttccggga atccggccgc tttcagcgca tccatagaac
     3001 ccgttgtacg gcgacgtgcc gtatccagtt cgttggcggt aatcgccatc accgcgcttt
     3061 ctttgacatc ccagccacgt ttctgcatct ctttatacag ttcctgaccc tgacgttcgc
     3121 caattttagt cgccgccatc atcaccagcg gaacggtatc cattggctta cctttggcgt
     3181 taacaaactg gtcatccacg gcaatgactt tcatatcgta gccacgcgct ttcgcgacga
     3241 tggcagagcc gagtttgggg tccggagtac aaataacgaa accttttgcg ccactggcag
     3301 ccaggctgtc gatcgcgttc aatgtttttt cgccatccgg cacggcaatc ttaataacct
     3361 caaaccctaa atccttcccg gctttatcgg caaacttcca ttcggtctgg aaccacggct
     3421 cttccggttg cttcaccagg aaaccgagct tcaggttctc cgccatagcg gattgtgaca
     3481 taacggctgc cagaccaatg gctgccaggg ctttagtaaa tttgtgcatg gttctctcca
     3541 gctttagtgt cgttttgtgt agggcaaaaa cgaatgacat tcgttaaatt aatcggaaaa
     3601 caaagcatta ccttttaact aaaagataag tgactgtgtt gacatagttt tagcgagaaa
     3661 ttaattctcc ataggagagc aatatcacat cgcagaatta cagtgagaac gtgcataaat
     3721 ttagcgggaa aagacataag ggaaagccaa tttgtcagac aaattgtcga atgcacagca
     3781 gattaatcca taagattagc ctggaaatcc ttgttgtctt tggtacccat gcgggatgtc
     3841 ttctttttaa ccagtcaata ggccgcatta cctggcgttg agtttttgaa atggtgtaat
     3901 aaccacaact caaagatgtg gaaaatgcgc gtcattcatt tcgccattaa ttatcaccgt
     3961 gctcattaat taacagaaca cgtataatga gagccatctc gcaaaaatga aaaaacgttt
     4021 tataaaatca tcacttcatc atgaattcaa attcatcgtt taatatcaac aagatacaaa
     4081 aagcactatc attaaaattc attgcagtta cattgatttc atcaatgaaa tgtaaaaata
     4141 tataaacttg atgatttaag cattttctta tacccgttca gacgttattc ttatttcaga
     4201 tcatcgtcag aattgactcc acgatcacat ttcggaccgg cagaaaggaa ttattctgca
     4261 aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat
     4321 taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt
     4381 acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca
     4441 ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta
     4501 actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt cccggtgaaa
     4561 aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcta
     4621 gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt
     4681 tcctgcgcga tctggaaaaa gaacagcagc atgatggtct gttgctgcaa accattcttg
     4741 atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac caacatgttc
     4801 tgaatgtcgt gtcacaccag ctgcattgat catcatcggc gctaatgcat tgcgccgatg
     4861 aaggttttga gaaaccgctg cctcatctgt ttgaagcagc ggttttttta atgggattca
     4921 ccctgtgggg taaacttgag ttcaataagc gcgatggctt tttggattgc ccgcatggtg
     4981 accgggtctg cggcggcggg atggttagta aagtcgatat tcttcagctg actggacatt
     5041 ttttcacgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg tttgataacc
     5101 aactggcaag caaccacatc agaaaccagt tcctgatcgg cattcagcgg ctgggacatc
     5161 gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc ttcagcgata
     5221 gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg
     5281 aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa catacttttc
     5341 aggattttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt gttattctct
     5401 ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatac aaatcacata
     5461 aatacccctt taatgttata aaaatgataa tcaaaaaaca gcccccctat ttctgacacc
     5521 tacagatggc aagaaatagc gcctgccagg cgtcttttcc ggccattgtc gcagcgctgt
     5581 aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact tctgattctg
     5641 actctttcat taataacgct ggcggggtgt acggttactc gccaggccca tgtgagcgaa
     5701 gttgatgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt gcagcatgcc
     5761 cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca ggaaggctat
     5821 acccacgcgg tcccctttgg tcagccagta ggcaactgta gcctttttgc gggttcctta
     5881 tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt ccctgtcttt
     5941 ctctaaatat tttaatcagc gaggggatct tcgctgatta aagaaatagc ggtaatgcgt
     6001 tttattccca ttcgtatttt taataattga agtttatatt ttaccttttg caaataataa
     6061 aataacaaat tatagtgacg ccacagcaac aaatataacc tttgtggagc actatcatgc
     6121 tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg tactcttcac
     6181 tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt gctgccgcgt
     6241 tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt gattacctga
     6301 ctgataccgg caatttaccg cgtattaata ccgttgaatc tccgtttgct gaatattcct
     6361 cacttgatga attattccag gaaacctata aacacgaaca attaatcacc cagaaaatta
     6421 acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat ttcctgcaat
     6481 ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt gataaattaa
     6541 gcctggcagg caaaagcggc gaaggtctgt attttatcga caaagaactc tctaccctcg
     6601 acacacaaaa ctaatgctct ccggcggcag gtttactgcc gccgtttcaa atcagtgacg
     6661 gcaaatctta ctctgatcgg tcgaaaaacc gtctttcgaa ggtataaatt tgccttttgc
     6721 tgccagaaac gctaccagct ctcccgctgt catcccttct gccgagcagg tgtgaaaacg
     6781 tgcctgttca ccaaaacgcg ctttaatcgc agcttccaga ctggcatgcg tatattgctc
     6841 gcctgattca atcatcatat ttaacacttc atgaccgtga atagagtcca tcgtccctcc
     6901 tcaaaaaaag cctagcgtag cgattgccgc ttatgaagac tttgcgccag cgcaggactg
     6961 aatgcttttt attgtacatt tatatttaca ccatatgtaa cgtcggtttg acgaagcagc
     7021 cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga cagaagaaag
     7081 cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg ggaaccacaa ttggcgcagg
     7141 catgctggca atgccgctgg ctgcggcagg tgttggtttt agcgtcacgt taatcttgtt
     7201 gattgggctt tgggcgttga tgtgctacac ggcgctatta ctgctggagg tgtaccagca
     7261 tgttccggca gataccggtc ttggcacgct ggcaaaacgc tatctgggac gctacggtca
     7321 gtggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcgg catacatcag
     7381 cggtgccggt gaattgttgg cctccagcat cagcgactgg acaggtattt caatgtcggc
     7441 aaccgctggc gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg tcgggacatc
     7501 gctggtcgat ttgtttaacc gttttctgtt cagcgccaaa attatttttc tggtggtgat
     7561 gctggtattg ctgctaccgc atattcacaa agtgaatctt ttaaccctgc cgttgcaaca
     7621 ggggctggct ctgtcagcaa tcccggtgat ttttacctca tttggttttc acggtagcgt
     7681 gccgagtatt gtcagctata tggatggcaa cgttcgtaag ctacgctggg tgtttataac
     7741 cggtagtgcg atccccctgg tggcatatat tttctggcag gtggcaacgc ttggcagcat
     7801 tgattcaaca acctttatgg gattgctggc taatcatgcc ggattaaacg ggctgttaca
     7861 ggcattacgc gaaatggtgg cctctccgca tgttgagctg gcagtgcatt tatttgctga
     7921 tttagccctc gccacgtcat ttctcggcgt tgcgttaggc ttatttgatt atctggctga
     7981 tttatttcag cgttcaaata ccgttggtgg acggttgcaa actggtgcaa ttacctttct
     8041 gccgccgttg gcgtttgcac tgttttatcc acgaggattt gtgatggcgc tgggttacgc
     8101 cggtgtggcg ctggcggtac tggcattgat tatcccttcg ctgttgacct ggcaaagcag
     8161 aaagcacaat cctcaggcgg gttaccgggt caaaggtggt cgtccggcgc tggtggtggt
     8221 gtttctctgt ggtattgctg tgattggcgt gcaatttttg attgcggcag ggttgttacc
     8281 agaagtgggg tgatcagata gcctcaaatt ccttattggg tgccagaatt aacgctgaca
     8341 cccaatttga cctcttaatg caggcagcac tgcttaaatt tcttaccact accgcacggg
     8401 caaggatcgt tacgccccgg tttctcttct gctttgatcg gttgctgaac agctttttcc
     8461 tgcggatgcg ccatccagta cgcatgtaga tcaagcgccg ccagtcgaat ggcatctacg
     8521 ctctcttcaa acgcttctgg cgacattttc tctacccgct cgaagttttc ctcagtaccg
     8581 tgcagcgcaa tcgcctccag cgctggtttt aacgaatcgg gcaacgttga ccagtcagaa
     8641 agtgccacgc cccgcatata gccaaagcac cactcctcaa caatcgtcag ctcgctgcca
     8701 tcaacttctc gcaagccgaa taacggctca aactgctccg ggaattcgtt cagacgctct
     8761 gcggtatcgg ccatatgttg aaaagccaga ttcataaagc gcgtcatctc tttctctgac
     8821 gcccagcgcg gcacatagtc agccccaccc cacacggcaa ccagccactg ttccggttca
     8881 atctcttgcg gagaactcaa caccgccgtc aataaaccgt ccagctccgc cacatcaagg
     8941 atggcgtggt cagtgttgta tttggtcaga atatcgtcca gccattccaa ctcactttcg
     9001 tttaacggtc ccgttttcat acgcttttcc ttgtggatct caactcgcca gcacctatct
     9061 tacatgccgg tccgtatcag agatactttt tgagtggctt tgctggtgat taaaaattaa
     9121 ggagggtgta acgacaagtt gcaggcacaa aaaaaccacc cgaaggtggt ttcacgacac
     9181 tgcttattgc tttgatttta ttcttatctt tcccatggta cccggagcgg gacttgaacc
     9241 cgcacagcgc gaacgccgag ggattttaaa tcccttgtgt ctaccgattc caccatccgg
     9301 gctcgggaag aaagtggagg cgcgttccgg agtcgaaccg gactagacgg atttgcaatc
     9361 cgctacataa ccgctttgtt aacgcgccaa attcttcagg cctttcagcc agacatccgc
     9421 tgacgccgat gtcttttaaa ctggagcggg aaacgagact cgaactcgcg accccgacct
     9481 tggcaaggtc gtgctctacc aactgagcta ttcccgcatt catcaagcaa tcagttaatc
     9541 acttgatttt attatcgtct ggcaatcagt gccgccgttc gatgcgttgc attctactta
     9601 cctggcgcga tgagtcaacg atatttttca ccacttttga ttgtttgctg aaaattacgc
     9661 cgaaacgatc actgttcaag caaatctgca cgcgcagcgc tcaaatattg caacattgac
     9721 cacagagtca gtaccgcagc cacaaagaaa agtgcaatac cggcgtactc aacccaaatg
     9781 ttcggacgcc acagcagcca ggccaacgcc accatctggg cagtggtttt cactttccca
     9841 atccaggaga cagccacgct actgcgttta cccaactccg ccatccattc gcgtagcgca
     9901 gaaataataa tttcacgggc gatcatcgtt gccgccggta aggtcaccca ccagctgtga
     9961 taatgctcgg ttaccagcac catggcgata gccacgagaa ctttatctgc gacagggtca
    10021 aggaaagcac caaaccgggt actctggttc cagcggcgtg ccagaaaacc atcgaaccag
    10081 tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca ggtgacaggc
    10141 agataaaaga ccaatacaaa gaatgggata aggatgacac ggaacagtgt aagcaacgta
    10201 gggatattaa attgcataat gacgggtaac tatctgttgt cagtaagatt acccctatgt
    10261 tgctacagag acatcaatgt ttcaacgacc agaagatctt ttctgccaga ccttgcgaaa
    10321 tacccggcac ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc aaaccgccca
    10381 tatatttcaa caacatttgc cggcgttttg gcccgacgcc ttcaatggtt tccagggaac
    10441 tggtattttt gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga tcatgtgatt
    10501 catcgcgaat atgctggata acatgcagcg cgggagaatc tggcggcaaa ctgaatccct
    10561 caccttccgg ctcaaagaac agcgtttcca gcccagcctt acgatctgct cctttggcaa
    10621 cgccaagtag cagcggatga tttttatccc atgagacatc cagttcggcg aagacatttt
    10681 tcgcctgcgc aagctggcct ttgccgccgt cgataagtat cacatccggg atcttactgt
    10741 cgtcgatggc tttaccataa cgccgacgca gcacctgatt catcgccgca taatcatcgc
    10801 ccggcgtgat gccagtaatg ttatagcgcc gatactccgc acgcagcggg ccgttagcat
    10861 caaacaccac acaggaagcg acggtttgtt cacccatggt atggctgatg tcaaagcact
    10921 ccatccgctt cacttccggc aatttcaaca cgctggcaag cgcggtcagt cgctggtgaa
    10981 cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg cgcgcgagtt
    11041 tcagataacg cgccctatcg ccgcgaggtt tggtttgaac attaatcttg cgtcccgcca
    11101 gttctgaaag ggaatcggcg agcagcgttt tatcgctaag attaaaatcg agcaggatct
    11161 cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa ctggcctacg aaggtttcta
    11221 ccacctcgct cagttccgta ccgccaggca ctttcgggaa atagctgcgg ctgccgagca
    11281 ctttgccctg acgaatgaac aatacgtgga cacaagccat gcccgcatcg aacgccacac
    11341 caataacgtc gaggtcgtcg ccggtattgg aaacgaattg tttttcggtg acgcgtcgca
    11401 ccgcctgaat ttggtcgcga atacgtgcag cttcttcaaa ctccagattc tggctggcag
    11461 tttccatacg actaatgagt tgcgtaagca cctgatcatc tttgccagac aaaaacaggc
    11521 gcacatactc gacctgctga gcgtattctt cttcactcac cagtccttca acgcacggtc
    11581 ccagacagcg ccctatctgg tattgcagac acggacgcga gcgattgcga taaacactat
    11641 tttcgcactg gcgaatgggg aaaatctttt gcagtagcgc cagtgtttca cgtacggcat
    11701 agccattcgg gaacgggccg aaatattcac ctttggcatg cttcgcacca cgatgcatcg
    11761 ccagacgcgg gtgggtatca ccactcagga agataaaagg atatgattta tcatcgcgta
    11821 gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc aacagcgctt
    11881 cggtttctgt gtgagtaacc gttacatcaa tttgctggat ctgggcgacc agcgcttcgg
    11941 ttttgcgcga agcgaggttg ctacggaaat agctggaaag ccgttttttc aggtctttcg
    12001 ctttgccgac atagataacc gtaccaccag catcgtacat gcgataaacg cctggctggc
    12061 tggttacggt ttttaaaaac gcttttgcgt caaactgatc actcactgac ttgataatgt
    12121 ctccgcatta cacagaccat ggcgaattgc caggtgagtc agctcaacat cgccatgaat
    12181 gtttagttta ctgaacatac gatagcggta gctgttcacc gttttcggac tgagattgag
    12241 ctgttctgag atctcattga ccttctggcc cttggtgatc atcagcataa tctgcaattc
    12301 acgttcagac aaactggcaa atgggctttc tgttttttct ggttcgatct ggcttaacgc
    12361 catttgttga gcgatgtcag aagcaatgta acgctgccct gaatagacag aacgaatcgc
    12421 actcacgact tcctgcggag ccgcgccttt gctgaggtag cccgcagcac cggcctgcat
    12481 gactttcgct ggtaaagggt tttctgtatg gacggtaagc atgatgattt tgacatcagc
    12541 tgtggaacgc gcgattttac gcgtcgcctc aagaccgcca atgcccggca tactcatgtc
    12601 cattagcacc acgtcaacgg catttgtccg gcaccactta acggcgtctt caccgcacga
    12661 tgcctcaccg acgactttta taccctttat atcttccaga atgcgtcgta tccctgcgcg
    12721 caccagttcg tggtcatcaa caagtagaac gttgatcaaa ggaatatctc cagaaatagg
    12781 gataacgcta ctgatagtta gtcattcgta tattagcggt ttttattgca actttgaaac
    12841 gttaaaaatg gtgggtattc gatttttctc tcgtttttgt caattcgatt gtccacagtt
    12901 atggtcacgc ccgtcaccaa cggcttaccg gatcgtttca cagtaagccc tacgatgaaa
    12961 tgtgacaaaa attgacttta ttcagcaaaa atgaaaatca gcccgctgaa tatgtaacat
    13021 taattaaccc gaacaatacg ggcaaaaaac attcatgata aaaatattta ttggtcatta
    13081 tattaacgta ttttatagca ctgccgatat cacgctcaaa aaacaaccac tgctattttt
    13141 agcaaagctt atggtatact ccgccgcctt aacatttttc accgcaaatt ttcattgcaa
    13201 catgacgagg aaaataaatg agtacgcctg atttttctac tgccgagaat aatcaagaac
    13261 tggcaaatga agtctcctgc ctgaaagcga tgctgacgct gatgctgcag gcgatgggac
    13321 aagctgacgc gggccgcgtg atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg
    13381 aaacccaggc tgcagtattt tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt
    13441 aatataaaac cggctgagag cgtgcctttc agccggtttt tgcatctggc acgcagaaag
    13501 aaaagcgatc aaattaagcc agtagcggcc gcgtaacagg ccacctgggt cttatttggt
    13561 gcattgattt ttttctgcat gtttttctga tggaaattga ccgtattctc agagattgac
    13621 aaaatcatcg ctatctctgc tgatgtcttc ccttccgccg tccatttcag aatttctttt
    13681 tcgcgcttgc tgaaattcat ctctggcgtc atcactattt catcatttaa acgcatcaga
    13741 gccatcagac tttcgcgcac cagtaactgc atttttaatt gcagttcatc actaagaatg
    13801 ggtatttcgc gagcgctgca acgggaaaag gacaaaaagc ccaacgcccg atttggcagc
    13861 attaaatact gagtgacacc gcggcgtaaa ccatgtgcgc gcgcggcttc ccataacggc
    13921 tgtgcttcgc tgaataagtc atcattccac attaaatggc cctgactaaa gttttcaggg
    13981 ttcagcaccg gatcaattgc gagaaagttt tttgcctgat aataactaac ccacgcctca
    14041 gggtaattgg tgtagaaagc cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat
    14101 aacgaatagt aatcgtactc cagctgctga gcctgaagct ctatttcatg gtagacctct
    14161 tctgcggcct ccatcctctg aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc
    14221 gtatcctgca tagtaaaccg caacgcccct gagagtgagt atcatttata atgataattc
    14281 aaactatcac ataaataaga tttatatata atttatatta ttcaggcaat gaattacttt
    14341 tgcaagccat cgcattctct tatgttatta atgagttatg ctgatttgtt aagcagtttt
    14401 atcacgcttg aaatggcgtc ccgccccgac aggtgaatcg tcggggctga ttttttctta
    14461 ttattgcagc agaaacttct cgaggaactg gcgggtgcga ggctgctggg gatcagcaaa
    14521 taacgctttt gctgccccct gctcgactat ccgcccctgg tccataaaaa tcgcccggtc
    14581 cgcaacatcc cgggcaaagc tcatttcgtg cgtcacaatc accatcgtgc gcttttcctg
    14641 cgccagctgg cggattgtgt tcaggacttc gcccaccagc tctggatcca gcgctgacgt
    14701 tggctcgtca aacaaaatca cctcaggatg cattgccagc gcacgcgcaa tcgcaacacg
    14761 ctgctgttga ccgccagaca aacgacgtgg atagctggtt tctttaccgg ccagcccaac
    14821 tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat
    14881 caccggccct tcaataatgt tttccagcac cgtacgatgc ggaaacaaat taaagttctg
    14941 gaagacaaac ccgacgtgct gacgcaactg gcgaatcagc gatttttgct gacttaatga
    15001 acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt cccgcttccg gttgttccag
    15061 cagatttatg ctgcgtagca acgtggtttt gccggaacca ctcggaccga taattgccac
    15121 cacttcgcca ggctttacct cgaggtcgat accgtgcagc accgtctgac cgtgaaattt
    15181 tttcaccagg ttcttaactt caatggcact cattttggtt ctctctcctg gcgattaagt
    15241 tgattctcaa aatggttctg caacgtcgat aatactgtcg ccatgatcca gtagatgagc
    15301 gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcttga
    15361 cggaacagct ccggcacctg gattgtcgcg gccagcgacg tatcctttac caggctgata
    15421 aagctgttcg acagcggcgg caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc
    15481 atagtctgcc atggcgtcat accaatactg gcagctgctt cccactgacc tttatcaata
    15541 gaagaaatgg cggcgcgcag cgtttcggcg gcataggcgg cagtgttcag cgacagacca
    15601 atcattgctg acggaatggg atctaattcg ataccaaact gcggcaggcc gtaatagatc
    15661 ataaacagtt gtgcgatgag cggcgtacca cgaaatatag agatataaaa acgcgccagc
    15721 cagcgcacgg gccagattgg cgacaggcgc atcagcgcga gaataaaccc gagtagtaag
    15781 ccaaaaaaca tgccgccgat actgagttgc agggtatacc cagccccttt caacaggaac
    15841 ggcaaggaat caataaccag ttgtatactt tcttgcatga gcgttcccgg agctaaacgt
    15901 ggggatgata ggcgaacagc gcaggcgcgc cgccggtatg aataaacaga atcggccctt
    15961 catctttgaa gcgtttctga ctgataccgt caatcagccc cgccatcgct tttccggtat
    16021 acacaggatc aagcagaatg ccttccagac gcgccagcaa tttcactgct tccatgcctt
    16081 cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc ccagagtaaa atttccgctg
    16141 atgcggtcag ctccagttct ttcgcaatcg cctgttgtag gttaaccact ttcggcaatt
    16201 gatcggcaac ggaacgcgac acggtcacgc caatcagttc gctttcaggc atcaggtgtt
    16261 ccagcccaac agccaatccg gcgtgagttc cggcactgcc cgatgcgacc accaccgacg
    16321 aaatattaac cgccccttca cactgttgcg cgatttccag cgcactctcc acataaccca
    16381 gcgcgcccag agcattagaa ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt
    16441 cgactcgcgt cgccagctct tccagttggg cattgggatc ggtcagtgcg tcgcacattt
    16501 caatctgggt attgaacaga tccagcaaca agcgattgcc gttggttaaa tagttttctg
    16561 cggttgtgcc aataggattt tccagcagcg ccacgcagtg cagaccgagt ttggcagcga
    16621 ctgcggcagt ctgccgcaca tggttagact ggatcgcccc ggcagtaatc agcgtatcgg
    16681 caccttcgcg tagagcatct gccgcgagaa attccagctt acgtaattta ttgccgccca
    16741 ttgccatggg ggtgacgtca tcccgtttga tgaaaatttc ccgtcctaga taatcagaaa
    16801 agcgcggcag atattcgagc ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg
    16861 ttaaattatg cagtggcata acagcctccg atgtgtgttg ttgtgatttt cttattatgc
    16921 acgctgaaaa cgcgtaaata aaaaaggcgc tagtgaaagc gccctttttt gtcattatgc
    16981 tgattatttg gtcacatcag caccaaacca tttttcggaa agggcttgca gagtgccatc
    17041 tttttgcatt tccgcaattg catcattcac cactttcagc aggtcttcat ttcctttacg
    17101 cagcgccaca ccagactcct gacgggagaa tgcttcaccg gttactgcca gcgtatcgtt
    17161 ggttttcttc accagatcca gcgccgccag acggtcaacg aggatcgcat cgatacggcc
    17221 tacgcgcaga tcctgatatt tggtcgggtc atcatcatag gtacgcacat cgacgccctg
    17281 aacattctga cgcagccact cttcatagtt ggtgcccaga ccgacaccca cttttttgcc
    17341 tttcagatca tcagccgttt taatggtgcc ttcgttacct tttttcacca gcgcctgaat
    17401 accggaaatg gtgtacgggg tagagaaatc gtattttttc ttgcgctcgt cagaaatggt
    17461 gacctgatta atcaccacat caatgcgttt agagtccagc gacgccagca taccgtccca
    17521 tttggtcggt tttagtgacg tctcaacgcc aagatgtttt gccagctgtt gggcaaattc
    17581 cacttcaaaa ccggttaatt tgccgtcatc cccctgaaaa ctgaacggcg gataagttcc
    17641 ttccagccct accagcagcg tgccgcgctc tttgacttta ttaagcagac cttcatctgc
    17701 aaaactttta acgctcatgc ccgcaaccag cgccacggcc atcacaccca tcaatgcctg
    17761 acgtcccaga tgtgctaatt tcatattcac cccgaatgtt gttatgtctg tttgcagtgt
    17821 agagccattg tttgtaaaca caaaaacaac tccgctacat cttattctta tttaatatat
    17881 atcagaagca ggcaggctgg aggatttctg cacaagtccg gtacacgttt ggcgctgata
    17941 gtgttgatac ttccgtaacg cgatgcggta attgttggtg ctggtggtgg gcagccacgg
    18001 ggcgaggatt tcatcaagga aaccgtcctg cagctgatcg agggaaatat tttgctcgtg
    18061 tagatgattc cccaaacggc gcaaacgaac gacatattca cgcaccgtac ctgggctcat
    18121 ttcagtttgc tcaaaaagaa attgcttaaa gccgataata tcgaaaaaat cactctgcgt
    18181 tttgcagtgt aaatcaccgc aaaatcgaca caatgccgcc catgattgtt gttccacttg
    18241 ccagccattt tcatcaagca gcgtgtccag gcgggaaatc tcgattttat tcactatttt
    18301 gccgtcgcga actaaggtaa tgcgatcgag taatttacgg caatgcgcgc aatgggtctg
    18361 gctgtgttta aagtctttaa gatagcggct taatggccgt cttttcaggt gctgcaccat
    18421 cattaagaac tcctggtagt caaagttaaa gtgcggcatt tactgacgtt ataacttacc
    18481 cagtttagtg cgtaaccgtt taatggcctg gctgtgtaac tgactgaccc gcgattcccc
    18541 gacctccagc actgcaccaa tctctttgag attcagctct tcctggtaat agagggttaa
    18601 taccagtttt tcgcgctccg gcaacgtttc gatagcttcc atcacccgct ggcgcagatt
    18661 actgtccagc agttgttgta gcgggttttc tcgctgatga tcatcagtaa ccagttcgat
    18721 gctatcgccg tgctcttcgc gccactcatc gtaggagaag agctggctgt tattggtgtc
    18781 gagcaacatt tggcgataat cggcaatatc gatccctaaa cgttccgcta cctcagtttc
    18841 cgtggcgttg cggccaagtt cctgctccag ttgccctatt gcctgtgcca cttcacgcgc
    18901 gttgcgtcgc acgctgcgcg gcacccagtc acggctgcga agttcatcca gcatagcgcc
    18961 acggatacgc tgcactgcgt aagttgtaaa tgccgttcct tgtagggcgt catagcgttc
    19021 gacggcatta agtaacccaa tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc
    19081 gggcagtcga acctgcaggc gcaatgcttc gtgacgcacc agcgggacat aacgctgcca
    19141 cagcgagtgt ttatccatta caccttcagc ggtatagagt gaattcacga taaacagccc
    19201 tgcgttatat gagttatcgg catgattatc cgtttctgca gggtttttaa tcggacgatt
    19261 agtgggtgaa atgaggggtt atttgtgggt taccggtaaa ttgcgggcag aaaaaacccc
    19321 gccggtggcg ggggagcacg ttgctgacaa attgcgcttt atgttgccgg atgcggcgta
    19381 aacgccttat ccggcctaca aaaatgtgca aattcaataa attgcaattc accttgtagg
    19441 cctgataagc gcagcgcatc aggcaatttg gagttgccgt cagtctcagt taatcaggtt
    19501 acgacgatta accctgcagc agagacagaa cctgctgcgg tacctggttg gcttttgcca
    19561 gcacggagtt accggcctgc tggatgatct gcgctttcga catattggac acttcggtcg
    19621 catagtcggc gtcctgaata cgggactgcg cttcagacag gttggtagtg gtgttgttca
    19681 ggttggcaat ggcagaatcc agacggttct gaacggcacc gagagaagag cggaatttat
    19741 caacagatgc aattgcatca tcaagagcct taagcgggtc agctgttaaa ttgcttggcg
    19801 cggttgtaga tgcaccaata gataaaacac cagcatcact gatattagcg gcattgaaag
    19861 ttgattgatt agttgttaca tctttggtct gaatgatata agcatcattg ccgcttttat
    19921 ctttatacac gacaagcgat tggttggctt taagtgcaga agcatccaat tttacatcgc
    19981 ggccaacagt agttacttcc gtcgctgcag taggagtagt tccactttca tttgtagagt
    20041 tgaagttaat ttcaccctta ctagtatcta cttcggcatc gtagtactta ctatttgtag
    20101 tgtcatttac atagtactta ccgtctttct caactaattt tagggtacct aacgatcctg
    20161 aagctttatc tttaaagtcg ccagcaccta ctgccacttg cttattgatg gtggccccat
    20221 ctttaacact ggtgcccaag tttgtagcat caaaactgga gaggtttagt gtagatgaat
    20281 cgatcttttt cagatcaata gtgatagtct gtccatcatt tgccccaacc tgaatgttca
    20341 ttttcccatc tttagccaaa acattcacgc cgttaaattg agtctgactt gaaacacgat
    20401 caatctcttc caggcgagat ttgatttcgt cctggataga agacaggtca gactcagagt
    20461 tagtaccggt ggtcgcctga acagtcagct cacgcacacg ctgtaagttg ttgttaattt
    20521 cggacagcgc gccttcggtg gtctgtgcaa cagaaatacc gtcgttggcg ttacgtgcag
    20581 cctgagtcag gcctttaatg ttagaagtaa aacggttagc aatcgcctga cctgcggcgt
    20641 catccttcgc gctgttaata cgcaagccag aagacagacg ctcgatagaa ctcgacagcg
    20701 cagactggtt cttgttgata ttattttgag tgatcagcga gaggctgttg gtattaatga
    20761 cttgtgccat gattcgttat cctatattgc aagtcgttga ttacgtattg ggtttccacc
    20821 cgtcggctca atcgccgtca accctgttat cgtctgtcgt aaaacaacct ttagaatttt
    20881 tttcacaaac agccattttt tgttagtcgc cgaaatactc ttttctctgc cccttattcc
    20941 cgctattaaa aaaaacaatt aaacgtaaac tttgcgcaat taaggccgat aaccccggta
    21001 ttcgttttac gtgtcgaaag ataaaaggaa atcgcatggc aagtatttca tcgctgggag
    21061 tcgggtcagg tctggattta agttcaatcc ttgatagcct caccgccgcg caaaaagcga
    21121 cgctaacccc catttcaaat cagcaatcgt cgtttaccgc taaacttagc gcctacggta
    21181 cgctgaaaag cgcgctgacg actttccaga ccgccaatac tgcattgtct aaagccgatc
    21241 ttttttccgc caccagcact accagcagca ccaccgcgtt cagtgccacc actgcgggta
    21301 acgccatcgc cgggaaatac accatcagcg tcacccatct ggcgcaggcg caaaccctga
    21361 ccacgcgcac caccagagat gatacgaaaa cggcgatcgc caccagcgac agtaaactca
    21421 ccattcaaca aggcggcgac aaagatccga ttaccattga tatcagcgcg gctaactcgt
    21481 cgttaagcgg gatccgtgat gccatcaaca acgcaaaagc gggcgtaagt gcgagcatca
    21541 ttaacgtggg taacggtgaa tatcgtctgt cagtcacatc aaatgacacc ggccttgata
    21601 atacgatgac actctcggtc agcggtgatg atgcgctaca aagttttatg ggctatgacg
    21661 ccagtgccag cagcaacggc atggaggtct cggtcgccgc ccagaatgcg cagctgacgg
    21721 tcaacaacgt cgccattgag aacagcagca acaccatcag cgacgcgctg gaaaacatca
    21781 ccctgaacct gaacgatgtc accacgggca accagacgct aaccatcact caggacacct
    21841 ccaaagcgca aacggcgatt aaagactggg tgaatgccta caactcgcta atagatacct
    21901 tcagcagcct gaccaaatac accgccgtag atgcgggagc tgatagccag agttctagca
    21961 atggcgcact gctcggcgac tccacgctgc ggacgattca gacgcagttg aagtcgatgc
    22021 tgagtaatac cgtcagttct tccaactata aaacgctggc gcagattggt atcacgaccg
    22081 atcccagcga tggcaaactg gaactggatg ccgacaaact caccgctgca ctgaaaaaag
    22141 atgccagcgg cgtaggtgca ttgattgttg gcgatggtaa aaaaaccggc atcacgacca
    22201 ccatcggcag caacctgacc agttggcttt cgacaacggg cattattaaa gccgctaccg
    22261 atggcgttag taagaccctg aataaattaa ctaaagacta caacgccgcc agcgatcgc
//
LOCUS       DAEVZA010000083        22268 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEVZA010000083 DAEVZA010000000
VERSION     DAEVZA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.083"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..130)
                     /locus_tag="LAF92_003134"
     CDS             complement(<1..130)
                     /locus_tag="LAF92_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Enterobacterial Common Antigen (ECA) polysaccharide
                     chain length modulation protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide chain length modulation protein"
                     /protein_id="HBI9792097.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALI"
     gene            complement(142..1245)
                     /gene="wecA"
                     /locus_tag="LAF92_003135"
     CDS             complement(142..1245)
                     /gene="wecA"
                     /locus_tag="LAF92_003135"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBI9792098.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(1485..2744)
                     /gene="rho"
                     /locus_tag="LAF92_003136"
     CDS             complement(1485..2744)
                     /gene="rho"
                     /locus_tag="LAF92_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBI9792099.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(2829..2930)
                     /gene="rhoL"
                     /locus_tag="LAF92_003137"
     CDS             complement(2829..2930)
                     /gene="rhoL"
                     /locus_tag="LAF92_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBI9792100.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(3071..3400)
                     /gene="trxA"
                     /locus_tag="LAF92_003138"
     CDS             complement(3071..3400)
                     /gene="trxA"
                     /locus_tag="LAF92_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBI9792101.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            3531..4796
                     /gene="rhlB"
                     /locus_tag="LAF92_003139"
     CDS             3531..4796
                     /gene="rhlB"
                     /locus_tag="LAF92_003139"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBI9792102.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            4930..6414
                     /gene="gppA"
                     /locus_tag="LAF92_003140"
     CDS             4930..6414
                     /gene="gppA"
                     /locus_tag="LAF92_003140"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBI9792103.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(6461..8482)
                     /gene="rep"
                     /locus_tag="LAF92_003141"
     CDS             complement(6461..8482)
                     /gene="rep"
                     /locus_tag="LAF92_003141"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBI9792104.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            8699..9148
                     /locus_tag="LAF92_003142"
     CDS             8699..9148
                     /locus_tag="LAF92_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBI9792105.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINVLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            9347..9628
                     /gene="ppiC"
                     /locus_tag="LAF92_003143"
     CDS             9347..9628
                     /gene="ppiC"
                     /locus_tag="LAF92_003143"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBI9792106.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(9715..11190)
                     /gene="ilvC"
                     /locus_tag="LAF92_003144"
     CDS             complement(9715..11190)
                     /gene="ilvC"
                     /locus_tag="LAF92_003144"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBI9792107.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            11340..12233
                     /gene="ilvY"
                     /locus_tag="LAF92_003145"
     CDS             11340..12233
                     /gene="ilvY"
                     /locus_tag="LAF92_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBI9792108.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(12285..13829)
                     /gene="ilvA"
                     /locus_tag="LAF92_003146"
     CDS             complement(12285..13829)
                     /gene="ilvA"
                     /locus_tag="LAF92_003146"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBI9792109.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRIFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(13832..15682)
                     /gene="ilvD"
                     /locus_tag="LAF92_003147"
     CDS             complement(13832..15682)
                     /gene="ilvD"
                     /locus_tag="LAF92_003147"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBI9792110.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(15747..16676)
                     /gene="ilvE"
                     /locus_tag="LAF92_003148"
     CDS             complement(15747..16676)
                     /gene="ilvE"
                     /locus_tag="LAF92_003148"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBI9792111.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(16696..16959)
                     /gene="ilvM"
                     /locus_tag="LAF92_003149"
     CDS             complement(16696..16959)
                     /gene="ilvM"
                     /locus_tag="LAF92_003149"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBI9792112.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(16956..18602)
                     /locus_tag="LAF92_003150"
     CDS             complement(16956..18602)
                     /locus_tag="LAF92_003150"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBI9792113.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(18605..18655)
                     /locus_tag="LAF92_003151"
     CDS             complement(18605..18655)
                     /locus_tag="LAF92_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792114.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(18742..18840)
                     /gene="ilvL"
                     /locus_tag="LAF92_003152"
     CDS             complement(18742..18840)
                     /gene="ilvL"
                     /locus_tag="LAF92_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBI9792115.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            19193..20713
                     /locus_tag="LAF92_003153"
     CDS             19193..20713
                     /locus_tag="LAF92_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBI9792116.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(20738..21076)
                     /gene="maoP"
                     /locus_tag="LAF92_003154"
     CDS             complement(20738..21076)
                     /gene="maoP"
                     /locus_tag="LAF92_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBI9792117.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWFKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            21195..22034
                     /gene="hdfR"
                     /locus_tag="LAF92_003155"
     CDS             21195..22034
                     /gene="hdfR"
                     /locus_tag="LAF92_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBI9792118.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(22130..22205)
                     /locus_tag="LAF92_003156"
     tRNA            complement(22130..22205)
                     /locus_tag="LAF92_003156"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22170..22172),aa:Trp,seq:cca)
BASE COUNT         5328 a         5892 c         5718 g         5330 t
ORIGIN      
        1 cgattaacgc aaacgccagc cccatgccaa taatccatag cttcccagcc cacaaggtac
       61 gaaacaaccc acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg
      121 gttgtgtcat cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg
      181 cagtctgcgt tttacgcgct taataaagcg agcaactttc caggcacgct taatgcaata
      241 tccatagagg aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg
      301 agaatattct gccagcacgc caatggaagc gagcagtgct gcggcaaggg taatcagcac
      361 aaacgcctga cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctgacggtc
      421 aggagagaat gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat
      481 tagcggaatg gcgattatcc acaaagcggt aaccgggctg atgggatggg ttttgccctg
      541 ggtcgtttcg agcaggatcc agataacggt aaaaccaatc agcgtactgc ccgcatcacc
      601 cataaagact ttgtagcggc gacccaggat accaaggtta agcatgatgt atggcaggat
      661 ggcggcgatc atcgcaaagc accagattgc gaggctggtt tgcccgtcga accacaaaat
      721 cataccgatt gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc
      781 aaccatgttg aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa
      841 cggtccgagc accatctccc aggagccaaa gatataaccc aggctactga gataaagttt
      901 gccgaacacc atcataacaa tgccaacagc ggcctgtatg gtggcacgga tttttacgct
      961 gatatcaaaa cggtcatcca gcgcgccaat gaaaacaagc acaccggcac aagcgagata
     1021 gagagatgca tgcggaatat agtaatcgac aattccgaac gtgaagcaaa tccctgcgta
     1081 aaccgaaatc cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt
     1141 atccactaaa ccgacttttt ttgccacttt acgggcaaaa aacagaaaca gtgtcgtgaa
     1201 taaaaaaata ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag
     1261 agaaaattat tagcagaagt ataaccacga agacctttat tcagaaggga aagtccgttc
     1321 cgaacccacc agtcctgtgc aaaaattaac catttgatat gcaaattgca taacatgcca
     1381 gcaaattcca ggaattttcc taacatcgcc agcgcggcat taagattaca gatataaaag
     1441 caaaacgcca cgtaaacacg tggcgttttt ggcataagac aaatttatga gcgtttcatc
     1501 atttcgaaga aatcgtcatt ggtcttagtc atggccagtt tattaatgag gaattccatt
     1561 gcatcgattt cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct
     1621 tcctgagtcg tgagcagctc ttctttacgg gtaccggaac ggttgtagtc gatagccggg
     1681 aagacgcgtt tttcagcgat cttacgagag aggtgcagtt ccatgttgcc tgtaccttta
     1741 aactcttcgt aaataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata
     1801 atggtcaggc tgccgccctc ttccacgtta cgcgccgcac cgaagaagcg tttcggacga
     1861 tgaagggcat tggcgtccac accaccggtc aacactttac ctgacgccgg aacaacagtg
     1921 ttgtaagcgc gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc
     1981 tcaaccaggc gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg
     2041 ggttcgtcaa aggtagaagc aacaacttca ccttttacca gacgctgcat ctcggttact
     2101 tcttccggac gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca
     2161 atgctctgag caatgttctg cagcagcata gttttaccgg ctttcggcgg tgccacaatc
     2221 agaccacgct gaccacgacc gataggtgat gccagatcca gtacgcgagc ggttaaatct
     2281 tccgtagaac cgttaccacg ttccatacgc agacgagagt ttgcgtgcag cggggttaag
     2341 ttctcaaaga ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact
     2401 ttcagcagcg caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta
     2461 tcaccagtgc ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga
     2521 ccggcgaggt aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc
     2581 agtacgccat caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa
     2641 ataatgtcct gcttacgcat acgagccagg ttttccagcc ccatattttc gccgagagtg
     2701 atcagctcag aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt
     2761 aaacttgggg taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct
     2821 gtttacggtc atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg
     2881 aaacgacaag acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa
     2941 acgggaagcg ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca
     3001 acactaagtt agcatgactc acgccgggcg tccagttttt agcgacgggg cacccgaaca
     3061 tgaaattccc ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg
     3121 cgcccacttt ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac
     3181 ggatgccata tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca
     3241 gtttgccctg atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac
     3301 cgcaccactc tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt acatccgtgt
     3361 caaaactgtc gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa
     3421 gcctggcgtg ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt
     3481 caccggatac gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa
     3541 cacatttaac agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagccc
     3601 ttgaaaaaaa agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc
     3661 tggcgggtcg tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc
     3721 ttacgtcaac gtttcattat cttctctctc atcctgcgat tgccgatcgc aaggtgaatc
     3781 aaccacgtgc cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc catgccgacg
     3841 cagaaccgct ggcggaagct accggcctga agctgggtct ggcttacggt ggtgatggct
     3901 acgacaaaca gctgaaagtg ctggaaagcg gcgttgacat tctgattggc actacggggc
     3961 gtttaattga ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtggtggtac
     4021 tggacgaagc cgatcgcatg tacgatctgg gctttattaa agatatccgc tggctgttcc
     4081 gccgtatgcc gcctgcaaac cagcgcctca acatgctgtt ctccgccacg ctttcgtacc
     4141 gggtacgtga actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg
     4201 aacagaaaac gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa
     4261 tgcgtttgct gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca
     4321 ataccaaaca ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg
     4381 gtttgttgac aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc
     4441 gtggcgatct ggatattctg gttgccaccg acgttgccgc gcgtggtttg catattccgg
     4501 cagtgacgca cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta
     4561 ttggtcgtac aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt
     4621 atgcattgaa tttgcctgct attgagacct atattggtca ctcaattccg gtaagcaaat
     4681 acaatccgga cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccacgta
     4741 caggcaatgg tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa
     4801 aatgtcatac tcaaaatcat tcaaactgta tcaagatggc aagagaatga atcccgatga
     4861 gcatactgta gtaagtgatt caggtgaaag aatgcagcca acacagagac agattgaagg
     4921 atgaagagta tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt
     4981 tttcatatgc tggttgtgcg cgaggtggct ggaagcatcc agacgctgac gcgaataaaa
     5041 cgcaaagtgc gtctggctgc tggcctgaat agcgaaaatg ccctgtctaa tgaagcaatg
     5101 gagcgcggtt ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg
     5161 caaattcgcg ttgtcgctac ggcgacatta cgtcttgccg tcaatgcggg tgattttatt
     5221 gccaaagcgc aggaaatcct cggttgtccg gtacaggtga tcagcggtga agaggaagca
     5281 cgtctgattt atcagggcgt tgctcacaca actggcggtg ccgatcagcg cctggtggtg
     5341 gatataggcg gtgccagtac tgaactggta accggcacgg gtgcacaaac cacctcgttg
     5401 ttcagcctgt cgatgggctg cgtcacctgg ctggaacgct attttgccga tcgtaatctg
     5461 gggcaggaaa attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc
     5521 gatgaattac ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg
     5581 ttacaggaaa tcatgatggc acaggggatg gatgaacgca ttaccctgga aaagttgcag
     5641 caattgaaac agcgagccat tcattgcggt cggctggagg aactggagat tgacgggctg
     5701 acgctggaac gtgcgttagt gttcccgagt ggtctggcga tcctgatcgc catttttacc
     5761 gaactgaata ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac
     5821 ggcatgttgc atctggccgt cgagcaggat attcgcagcc gtacgctgcg taatattcag
     5881 cgccgcttta tgatcgatat tgatcaggcg cagcgcgtag ccaaagttgc ggctaacttc
     5941 ttcgatcagg tggaaaagga atggcatctt gaagcaataa gccgcgattt gctcatcagc
     6001 gcctgtcagc ttcatgaaat cggcctgagc gttgacttta aacaagcgcc gcaacacgct
     6061 gcttatctgg tgcgtaatct ggatcttccc ggttttaccc ccgcacagaa aaaattgctg
     6121 gcgacgctac tgctcaacca gactaatccg gtcgatctct catcgctgca tcagcaaaat
     6181 gccgtaccgc cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc catcattttt
     6241 gccagccgtc gccgtgacga tctcgtgcca gagatgacat tacaggctaa ccatgaactg
     6301 ttgaccttga cgcttccgca aggttggcta acccaacatc cgctgggtaa agagattatt
     6361 gctcaggaaa gccagtggca gagctatgtc cactggccgc tggaagtgca ttaatgagta
     6421 agtgccggat gcgatgctga cgcatctttt ccggccttga ttatttccct cgttttgccg
     6481 ccatcatcgc tttcagattc gccagatggc tttgcccttt ctgcatccgt tcttcggcgc
     6541 tgaccacttt gcgctcctgt tcccaaatca gatcatcctg cggcagctcc agcaaaaagc
     6601 ggctcggctc cgggcgcacc agctcgccgt actgacggcg ttctttacac agcgtaaagg
     6661 tcaattcctt ctgggcgcgg gtaatgccga cataggccag ccgccgctcc tcatcgatat
     6721 tatcttcatc gatgctgctc tggtgcggca aaaacccttc ttccataccg accatgtaga
     6781 cataaggaaa ctccagccct tttgacgcgt gcagagtcat cagttgcacc tgatccagct
     6841 cttcttcact ctcaccacgc tccatcatgt cgcgcaaagt aaagcgcgtc accacctggg
     6901 tgagcgtcat cggctcatcc agttcgctgc cttccagcat ctccgtcatc cagctaaaca
     6961 gttggttgac gttcttcatg cgcatttcgg cggctttcgg gctgggcgat gtttcgtaca
     7021 gccaggattc ataatccatg ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct
     7081 ccgccagacg ctggatttct gccaaccagt gagtaaagcg ggtcaatgct tcataaccac
     7141 gtccgctaag cgtctgactc aggcccatat caaagctggc ggtaaacatg cttttattgc
     7201 gcgtcatcgc ccactcaccc agctttttca gcgtcgccgg gccaatctct ctcttcggcg
     7261 tgttaacgat acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg cgcagataag
     7321 ccagcaagtc cttgatttca ggacgagaga aaaacgacgt accaccagat attttgtatg
     7381 ggatgcggtt ttgcatcagg aatttttcaa acacccgcga ctgatggtta ccgcgataaa
     7441 gaatggcgta atctttgtac tgcgttttat tgacgaagtg atgggcgatc agctcgccag
     7501 taacgcgctc ggcttcatgt tcctcgttat tcgcgcttaa aacttttagc tctgtgccgt
     7561 aacccagttc ggagaacaga cgcttttcaa agacgtgcgg attattggcg atcaggatgt
     7621 tcgccgcttt caaaatacgt ccggaagagc ggtagttctg ctcaagctta atcaccttca
     7681 gcgccggaaa atcctgactc agcagcacca agttttgtgg acgtgcgccg cgccaggagt
     7741 agatcgactg gtcgtcatcg cccaccacgg taaagcgcgc gcggctgccc accagcagtt
     7801 tcaccagttc atactggctg gtgttggtgt cctgatactc atccaccagc agatagcgaa
     7861 ttttgttctg ccagcgctcg cggacttctt cattgcgttg cagcagcaac gtcggcaata
     7921 aaatcagatc atcgaagtcg agaacattgc acgctttcag gtgtgcatca tacagcccat
     7981 aacaatgggc aaaaatacgg tcccgctcgc caatcgcact ggctgccgcc tgggacggtg
     8041 ttttgagatc gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg agaactttgt
     8101 catcttcaat cagcccttcg gtcaactctt tgagcaacgc cagctgatcg gtatcgtcaa
     8161 acagcgaaaa gttcgccttc atccccagcg ccgcatactc acgcttgatg atatctagcc
     8221 ccagcgtgtg gaaggttgag atcatcagcc cacgcgcctc tttgcgaccc agcgtctgtc
     8281 cgacacgctc tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc
     8341 gcgcctgata accgcaaccg cggatcagat gggcgatttt attggtgatc acacgagttt
     8401 taccggaacc cgcgcccgcc agcaccaggc aggggccggt aacgaattcg acagcttgtt
     8461 gttggccggg gtttagacgc ataggtgtat tgctcaatct tcgaacgggg ggaggattgt
     8521 agcagaatcg catttgaatg tgaccgcgtg taagccgcta accggttggc tgcggatgtt
     8581 taaatcagag agagaagacc gaaatcggag ttcatcgtga atccattaga cttagaaaat
     8641 atcgggtcgt aacgttgtgt tattactttt tcagagttta ctaaggagag gtttactcgt
     8701 ggcgattaat ttcagcccta aggttggtga aatacaggaa tgcaactttg gtaactatcc
     8761 tgttatccag aatggacagt tcagcaccac gtattacgat ggccgcattc ctcctgaaat
     8821 gataaaaaac aggttagttg tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt
     8881 cgttccatta tcaaccactc gcgatcatga caagttaaat cgtggaatgc atgttgaaat
     8941 agccagtaat gttatcaatg tcctgcaatt ctttgatcag cagattcgtt gggccaaagc
     9001 cgacctggta caacaagtca gtcgaaaccg attaaataga gcaaggactt acagagggta
     9061 tcttaatcaa tgtttgccac atgagttggt tgcagacata cagagggcgg tcatcaagtc
     9121 aatcaatgcc atatcgctca taaattgaca ataaaataac catccccgat aatgactttg
     9181 tagcccatag aggccctaag ttttctcccc ttgagggacc tgcgaagccc ggattttccg
     9241 ggcttttgcc atatatgtac cagcccctcc ctgactgaca attacgttag catctgcgga
     9301 tcatgctaaa atcgccgcct gacaattatt cactcaaggc acgatcatgg caaaaacagc
     9361 agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc tcgagcagat
     9421 taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc catcaggcaa
     9481 acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt tcgataaagt
     9541 tgtgttctct tgtccggtac tggagccgac cggcccgctg cacacccagt tcggttacca
     9601 catcattaag gtgctgtacc gcaactaata gcaaggcctt ctccaggaga aggccttgag
     9661 tgttttctcc ctctccctgt gggagagggt cggggtgagg gcatcagcgc gcacttaacc
     9721 cgcaacagca atacgtttca tatctgtcat atagccgcgc agtttcttac ctacctgctc
     9781 aatcgcatgg ctgcgaatcg cttcgttcac atcacgcagt tgcgcgttat ctaccgcgcc
     9841 ttccggaata gctttaccca agtcgcccgg ttgcagctct gccataaacg gtttcagcaa
     9901 cggcacacaa gcgtaagaga acagatagtt accgtactca gcggtatcag agataaccac
     9961 gttcatttcg tacagacgct tacgggcgat ggtgttggca atcagcggca gctcgtgcag
    10021 tgattcataa tatgcagact cttcaatgat gccggaatcg accatggttt cgaacgccag
    10081 ttcaacgccc gctttcacca tcgcgatcat cagtacgcct ttatcgaagt actcctgctc
    10141 gccgattttg ccttcatact gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg
    10201 ccaggtcagc agtttcttat catcgttggc ccagtccgcc atcataccgg aggagaattc
    10261 gccggagatg atgtcgtcca tatgtttctg gaacaggggt gccatgatct ctttcagctg
    10321 ttcagacagc gcgtaagcac gcagtttcgc cgggttagag agacggtcca tcatcagggt
    10381 gatgccgccc tgcttcagcg cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc
    10441 tgcgtatgcc ggatcggtgc cctcttccac cagcttgtcg aagcacagca gcgagccagc
    10501 ctgcaacata ccgcacagga tggtttgctc acccatcagg tcagatttca cttccgcaac
    10561 gaaggacgat tccagcacgc ccgcacggtg accaccggtt gctgccgccc atgctttagc
    10621 gatcgccata ccttcgcctt tcggatcgtt ttccgggtga acggcaatca gcgtcggtac
    10681 gccgaatcca cgtttgtact cttcacgcac ttcggtgcca gggcatttcg gcgcaaccat
    10741 gactacggtg atatctttac ggatctgctc gcccacttcg acgatgttga aaccgtgcga
    10801 gtaacccagc gccgcgccgt ctttcatcag tggctgtacg gtgcgcacta catcagagtg
    10861 ctgcttgtcc ggcgtcaggt taaccaccag atccgcctgc gggatcagtt cttcgtaagt
    10921 accaacttta aaaccgtttt cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc
    10981 ttctttacgc agagcgtagg agatatcgag accagaatca cgcatgttca ggccctggtt
    11041 cagcccctgt gcgccacagc cgacgataac cactttttta ccctgaaggt agctcgcgcc
    11101 gtcggcgaat tcgtcgcgcc ccataaagcg acatttgccc agctgtgcca gctgctggcg
    11161 caggttcagt gtattgaagt agttagccat ggtgattcct cgtgatgttg tgcttcttat
    11221 tgttcggttc gcgtttgcga gattgaattc actatatgac aggaaattta ttgcggaaat
    11281 tgatatattc acaacgtcac attgcaattt ctgcaacgtc aacattgagg gctgcgccat
    11341 tggatttacg cgatctgaaa accttcctgc atctggcgga aagccgccat tttggccgca
    11401 gcgcgcgggc gatgcatgtt agcccatcca cgctctcacg gcagattcag cgcctggaag
    11461 aagatctcgg tcagccgctg tttgtgcgcg ataaccgcac ggtaacgctg actgaagcgg
    11521 gcgaagagct gcgcgttttc gcccagcaaa cgctgttgca gtatcagcag ctgcgccaca
    11581 ccatcgatca gcaagggccg tcgctctctg gcgagttaca tattttctgc tcggtgaccg
    11641 ccgcttacag ccatctaccg ccgattctcg accgcttccg cgcggaacac ccgtcggtgg
    11701 agattaaact tactactggt gatgcggcag atgcgatgga aaaggtggtc actggtgaag
    11761 cggatctggc gattgccggg aaaccggaga cgctacccgg cgcggtggcg ttttcgatgc
    11821 tggagaatct ggcagtggtg ttgattgcac ccgcattgcc ctgcccggtg cgtaatcagg
    11881 tttctgtcga aaagccggac tggtctacag tgccgtttat catggccgat cagggaccgg
    11941 tacgccgccg cattgaactg tggtttcgac gcaataaaat cagcaacccg atgatttacg
    12001 ccacggttgg cgggcatgaa gcgatggtat cgatggtggc gctcggctgt ggcgtggcat
    12061 tgttgccgga ggtggtgctg gaaaacagcc cagaaccggt gcgtaaccgc gtgatgattt
    12121 tagagcgcag cgatgaaaaa acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc
    12181 tgcatgagcc gctgattgag gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc
    12241 gcgcgggtag gcctgataag cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag
    12301 aacctgaacg ccggattatt ggtttcgtcg tggcaatcgt agcccagctc atttagccgg
    12361 gtttcgaaat ccggttcatg gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg
    12421 gtgccatggc tgcgatagtg gaacaaagaa atgttccagt gcgtacccag cgtgttgagg
    12481 aagcgcagca gcgcgcccgg tgattccggg aattcgaagc tgtagaggcg ttcctgcaac
    12541 ggatgtgacg gacgcccgcc gaccatatag cgcacatgca acttcgccat ttcgtcgtcg
    12601 gagagatcca ccacgctgta gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct
    12661 tcgaggccgc ggctcaggcg cacgccgaca aagatgcagg cgtttttggc atcggcaaaa
    12721 cggtagttga actcggtgac cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg
    12781 cctttttctt ccggaatggt caccgccaac aacgcttcac gctgttcgcc cagttcgcag
    12841 cgttccgaga cgtagcgcag gccgtggaag ttcacgttgg caccggaaag aatatgcgcc
    12901 agccgttcgc cgcgaatgtt gtgctgggcg atatattttt tcattcccgc cagcgccagc
    12961 gcgccagagg gttccgccac cgcgcgcaca tcttcgaata aatccttcat cgccgcacag
    13021 atcgcatcac tatcgacggt gatgatgtcg tcgagatact cctggcataa acggaaggtt
    13081 tcgtcaccga tgcgttttac cgctacgcct tcagcaaata gccctacgcg cggcagatca
    13141 accggatgac ccgcatccag cgctgctttc aggcaggcgg agtcttccgc ttctacggcg
    13201 atcactttga tttgtggcat cagttgtttg atcagcaccg caacgcccgc cgccagaccg
    13261 ccgccgccga ctggcacaaa tatgcggtcg agatgggcgt cctgctggag cagttccagc
    13321 gccagcgtgc cttgcccggc aatcaccatc ggatggtcga acggcggcac ccaggtgaac
    13381 ccctgctgct gagacagttc gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga
    13441 agcagcactt cgccgccgaa tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt
    13501 ggcataacga tcagggcctt cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg
    13561 tggttacccg cagaagcagt gatcacgccg tgcgcttttt gttcttccgt caggcccgcc
    13621 atcatggcgt atgcgccgcg cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc
    13681 accagaatga cgttatcaag acgcgacgac agtttttcca ttttttgtag cggcgtaacc
    13741 tgcgccgcct cgtaaaccgg cgcacgcagc actgctctta aatattcggc accttccgga
    13801 gcaccggaca ggggttgcga gtcagccatt attaaccccc cagtttcgat ttatcgcgca
    13861 ccgcaccttt gtcggcgctg gttgccaggc tggcgtaggc gcgcagggca aaggaaacct
    13921 gacgttcacg gtttttcggc gtccaggctt tgtcaccccg agcttcctgc gcttcacgac
    13981 gcgccgccag ttcggcatcg cttacctgta actgaatgcc acggttcggg atgtcgatag
    14041 cgatcaggtc accatcttca atcaggccaa tgctgccgcc gcttgccgct tccggtgaga
    14101 cgtggccgat ggaaagacca gaggtgccgc cagagaaacg accgtcggtg atcagcgcac
    14161 aggctttacc gagacccatt gatttcagga agctggttgg gtagagcatt tcctgcatgc
    14221 cagggccgcc tttcgggcct tcatagcgga tcaccaccac atcgcccgcg acaactttgc
    14281 caccgaggat cgcttctacc gcgtcgtcct ggctttcgta cactttcgcc gggccggtga
    14341 atttgaggat gctgtcatcg acgcccgcgg ttttaacgat gcagccgttt tctgcgaaat
    14401 taccgtagag caccgccagg ccgccgtctt tgctgtaggc gtgttccagc gagcggatac
    14461 agccattggc gcgatcgtca tcgagagaat cccaacggca atcctgcgag aatgcctgtg
    14521 tggtacgaat acccgcaggg ccggcgcgga acatattttt taccgcgtca tcctgggtca
    14581 gcataacgtc gtattgttcc agcgtttgcg gcaacgtcag gccaagtacg tttttcacat
    14641 cacggttcag taaccccgcg cgatccagtt cgccgagaat accgataaca ccaccagcac
    14701 ggtgaacatc ttccatatgg tatttctggg tgctcggcgc aactttacac agctgtggaa
    14761 ccttgcggga aagcttatcg atatcactca tggtgaagtc gatttccgct tcctgcgccg
    14821 ccgccagcag gtgaagtact gtgttagtcg atccacccat cgcgatatcc agcgtcatgg
    14881 cgttttcaaa cgccgcctta ctggcgatat tacgcggcag tgcactttcg tcgttttgct
    14941 cgtaataacg tttggtcaat tcaacaatgc gtttaccagc attaaggaac agctgcttac
    15001 ggtcggcgtg ggttgccagc agcgagccgt tgcccggctg cgacaggccc agcgcttcgg
    15061 tcaggcagtt cattgagtta gcggtaaaca tcccggagca ggaaccgcag gtcggacacg
    15121 cggaacgttc aacctgatcg ctctgggagt cagatacttt cgggtctgcg ccctggatca
    15181 tcgcatcaac cagatcgagc ttgatgatct gatcggaaag tttggttttc ccggcctcca
    15241 tcgggccgcc ggaaacaaag atcaccggaa tattcaggcg cagggaagcc atcagcatcc
    15301 ccggggtgat tttgtcgcag ttagagatgc agaccatggc gtcggcgcag tgggcgttga
    15361 ccatatactc aacggaatca gcgatcagtt cgcgagatgg cagtgaataa agcatccccc
    15421 cgtggcccat ggcaatccca tcatccaccg caatggtgtt gaactctttg gcaacgccgc
    15481 cagccgcttc aatttgttcg gcgaccagtt taccgagatc gcgcagatgg acgtgacccg
    15541 gtacaaattg ggtgaacgag ttcacaaccg cgataatcgg cttgccaaaa tcggcgtcgg
    15601 tcattccggt ggcgcgccac agcgcacgag cacccgccat attacgacca tgagtggtgg
    15661 tggcggaacg gtacttaggc atactttatt tactcccagt gtctgtctcg taaatgggac
    15721 ggtgcgtgcc gtcccatttt ttgtatttat tgattaactt gatctaacca gccccattta
    15781 tcttcggttt cgccagtgaa gaggccgaag aaggcttgct gaatgcgttt ggtaaccggg
    15841 ccacaacggc cttcgccaac ctgaataccg tctacgctgc gcactggcgt gatttctgcc
    15901 gccgtaccgg acataaacac ttcatccgcc aggtacaggg attcgcgcga cagcacctgc
    15961 tcacgtactt caattcccag ctctttcgcc agtttgatga tggcatcacg ggtaataccc
    16021 ggcagcgcgg aggaggtgaa cggtggggtg aacagcacac catctttcac ttcaaacagg
    16081 ttttcgcctg cgccttcaga gatataaccg ttcacatcca gcgcgatacc ttcctgataa
    16141 ccgtggcggc gcgcttcgct acccaccagc agggaagaga ggtagttacc accggctttt
    16201 gccgccgtcg ggatggtgtt tggtgctgcg cggttccagg aggaaaccat cgcatcgatc
    16261 ccctgctcca gcgcttctgc gcccagatac gctccccacg ggaaagcagc gataatcacg
    16321 tcggttgagt atcccgctgg cgggtttact cccatgccaa catcaccgac gaagatcagc
    16381 ggacggatat aggcgctggt gagattattt ttgcggatca cgtcacgaca agcttccatc
    16441 agctcatcaa tgctctgcga aaccgggaag cgatagattt tggcggagtc atgcagacgc
    16501 tgcatatgct cacgatggcg gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg
    16561 ccttcaaaaa ccgaagtgcc atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct
    16621 tcccagcgaa ccatctcccc attgaaccaa atgtaatcag ctttcttcgt ggtcattttt
    16681 atattccttt tgcgctcagg cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc
    16741 aacgtgtgcg acgtccacca gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg
    16801 gctggcaacg gtcaattcga tatttatatt ttgtgcatcg ctggcggcgg ccatattcat
    16861 tgagcagacg tggaaaccac gatgacgcac cacgcgtaaa acacgttcta aggtttccgg
    16921 attgaagcga gccgatacat tgacctgatg ttgcatcatg ataatttctc caacatttct
    16981 gaattactgg caccaggcgg caccagcggc cagacgttct caagttcgtc gattgagaca
    17041 tgaagcaggt atggcccatc actgttcagc atggtgtcga gtgccgcttc aacctggtct
    17101 ttacgggtga tgtgttggcc agggatgccg aaggcgctgg ctaacatgag aaaatcgggg
    17161 ttatcggtaa gggtggtttc gctatatcgt tcctggaaaa acagttgctg ccattgtcga
    17221 accatcccta accgttggtt atcgagtaag acgattttca acggtaactg cttacgtttt
    17281 acggtgccca gctcttgtac attcatcatg aaagagccgt caccggagat acagacgacg
    17341 gtatcgttcg gtcgcgcgac ttgcgcgcca accgccgccg gtaaaccgaa acccatggtg
    17401 cctaagccgc tggaggtgat gaaattttcc gggcgagtat gggcgatgtg ctgcgcagcc
    17461 cacatctggt gctgccccac atctgtggtc acgacgcaat ccgcaggttt acgatccgac
    17521 agttgtttta acaacaatgg cgcgtagata gcgtcaccgg gatggtcgta acgccaggta
    17581 tgttcatcac gcagctgcgc gcagtattgc tgccagtcat tgatatttaa cggctgctgt
    17641 aatgctggta acagagcatt taaatcacct tgtaatgcca catgtgcctg acgcagcttg
    17701 ttcatttctg ccgggtcgat atccatatgg ataacactgg cgtgtggtgc gaaggtgttc
    17761 agtttgccgg tcacccggtc atcaaaacgt gcacccacgg cgatcagcaa gtcgcactcc
    17821 tgcaccgcga agttcgccgc tttggtgcca tgcattccca gcatgcccag gtagtacgga
    17881 taatctgctt caactgcgcc cagccctttc agcgtgcagg tggcaggcat ttttgtggta
    17941 gcgagaaatt ctcgtaaagc aggaactgcc tgcgccatac ccacgccacc accaacgtac
    18001 agcatcggtt tttgcgcttt tgccagcatc tggcgcgctt gctcaacttc ggcatggggg
    18061 aaagtcactt cgttttcaac ggtggtgaac cacggttcca gatcgccgct ggctaactgg
    18121 atatcttttg ggatatcgac cagaaccgga ccaggacgac ctgagctggc aacgtcgaat
    18181 gctgcagcca tgatgcgcgg caactcttcc agcgactgca ccagaaagct gtgcttggta
    18241 caggctaacg acaatcccag gacatccact tcctgaaatg cgtcggtgcc gataaacggt
    18301 gcggacacct gaccagtgat ggcaacgaca gggatggaat ctaacagtgc gtccgcaagc
    18361 ccggttatca ggttggttgc gcccggacca gacgtggcga tacatacgcc agttttaccg
    18421 gtagcacggg cataaccgat agccgccatt gccgcaccct gctcatgtcg gcacagcaag
    18481 tgctccacgc cgccgtcata caatgcatcg taaaccggca taattgcgcc acccggataa
    18541 ccgaaaacgg tgttcacacc ctgtgcccgc aacgcatgta ccacccactg tgcgccattc
    18601 atagttagtt ccccgtcctg aatcttgaga aacagaattt tgtgctgtta ttcattgtct
    18661 gctcctcggt tatgttttta aggtcaaaaa aaacccccgg acctttcggt gcgggggtct
    18721 tagttcgtta aggcttgatt tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg
    18781 gataataatc accaccacgc taatcacgac caggctaatc actcgtagaa gggctgtcat
    18841 tttgtctttt cttgcatctt gttcgaagga atgcctaaag agttaccata ggttttgtaa
    18901 atagtacaag atattttttg gccgattttt atgatgcgtt taataatttc aatataaatt
    18961 gttgtttttt aaaaataaaa tagtgagtga aaaaaattca atttcagtat gaatgttgtt
    19021 cccttcctcg tagttcatcc tttgtccgct gcatttttgc gagcatcatt ccaccgccag
    19081 aacaactcaa cttcacttgc acaaataaac aggaatcagc cactaaaatt cacgaattag
    19141 cgggctacag cagcgatatc ttatgcacat aatggtgact caaggagggc ttatgtcact
    19201 gtcaattgtt catacccgcg cagccctggg agtaaatgcg cccccgatca ctgttgaggt
    19261 acatatcagt aaaggtctac ccggcttaac gatggtgggc ttaccagaaa caacggtaaa
    19321 agaagctcgc gatcgcgtgc gcagcgccat tatcaatagc ggatatgaat atccggccaa
    19381 aaaaatcacc atcaacctgg ctccagccga tctgccaaaa gaagggggac gatatgattt
    19441 acctatcgcc attgcgttgc tggcggcctc agaacagctt acagccaata agttagatga
    19501 atatgaatta gtcggagaac tggcgcttac aggcgctctg cgtggcgttc ccggtgcaat
    19561 ctccagtgca actgaagcta ttaagtcggg cagaaaaatt atcgtcgcga aagataacga
    19621 agatgaagtg gggctaatta acggtgaagg atgcctgata gccgatcatc tgcaggctgt
    19681 ctgtgcgttt ctggaaggta agcacgctct cgaacgcccg aaaccaactg atgcagtatc
    19741 ccgggcgcta caacatgatc tcagtgatgt tgtcggtcag gagcaaggaa agcgaggact
    19801 ggaaattacc gccgctggcg ggcacaacct tttactgatt gggccgccgg gaacaggtaa
    19861 aacaatgctc gccagccgta ttaatggcct gttgccagat ttaagcaatg aagaggcact
    19921 ggagagcgct gcgatattaa gtctggtaaa tgctgaatca gtacaaaaac aatggcggca
    19981 gcgcccgttc cgctcaccgc atcacagtgc gtcgttaact gcgatggtgg gcggtggtgc
    20041 aattccaggg ccaggtgaaa tttcgttggc gcataacggc gtgctttttc ttgatgagct
    20101 acctgaattt gaacggcgta cactggatgc cttgcgagag ccgattgaat ccgggcagat
    20161 ccatctttca cgcacacgag ccaaaataac ctatccagcc cgtttccagc ttgttgcggc
    20221 gatgaatccc agccctaccg gacattatca gggaaaccat aaccgctgca cgccagaaca
    20281 gacattacgt tatctcaacc ggctctcggg gccctttctc gaccgcttcg atctctcact
    20341 ggagatccca ttaccacccc ccggcatttt gagtaaaacg gtagtgccgg gagaaagcag
    20401 cgccaccgtt aaacaacgcg taatggccgc cagagagcgc caatttaagc ggcagaataa
    20461 actgaatgcc tggctggata gtccggaaat acgccaattc tgcaaacttg agagcgaaga
    20521 tgcgatgtgg ttggaaggaa cactgatcca tctggggtta tcgattcgtg cctggcagcg
    20581 gttattgaaa gttgcacgaa ccattgctga tattgatcag tctgacatta tcacacgtca
    20641 gcatttgcag gaggcagtta gctatcgtgc gattgaccgt ttgctcatcc atctgcaaaa
    20701 actactgaca taaaaaaagg gcatttcgcc ctttttatta atcatcagaa tcggtgtagt
    20761 cttcagcacc ttcaacctgc ggtttaccgc cggaaagggt gtgaaaacgt tttggacgct
    20821 taatacgcgt catatacttg aaccacacgc gttctgcttc tgtcactggc tcacgttcgc
    20881 cacggcatac tgctacgaag agtttctctt cctcggtaac cggctcgcgt ttgccaagat
    20941 ccaactcatt gaaggcataa ccatgacgct caagcagttg tgcctctttg atggtgaaat
    21001 caccatgacg agagaatcca cgtggataat gtttattgtc gaaatatcga ttagtcgtcg
    21061 taaagctttc cgccatcctg cacgctccta attctttgac cgagctagtt atggcgcgga
    21121 gtattagtta cgcttgacag agtgtaaaac aaaacattta aatcataacg acaaataatt
    21181 ttgcggagag cactgtggat acggaattgt taaaaacttt cctggaagtt agccgaacgc
    21241 gtcactttgg tcgagcggct gaatcgctct atctgaccca gtcagcagtg agctttcgaa
    21301 tcagacaact ggaaaatcaa ctgggtgtga accttttcac ccgccacaga aacaatatcc
    21361 gtttaaccgc tgccggtgaa aaactactgc cttatgcaga aacgctcatg agcacctggc
    21421 aggccgcccg caaggaggtg gcgcatacct cacgacataa cgagttttct atcggtgcca
    21481 gcgcctcatt gtgggaatgt atgcttaatc agtggctggg acgtttgtat caaaatcagg
    21541 atgcccatac aggcttacag ttcgaagcgc gaattgccca acggcagtct ctggtaaaac
    21601 agctgcatga acgccagctt gatcttctta ttaccactga agcgcccaaa atggacgaat
    21661 ttagtagtca gttgctggga tatttcactt tagcgcttta taccagtgcc ccttcaaaac
    21721 taaagggaga tcttaattat ctgcgacttg agtgggggcc agattttcaa cagcatgagg
    21781 caggtttgat cggtgctgac gaagtgccca ttctgacaac cagttctgct gaactggcac
    21841 agcaacagat tgcgatgctt aatggttgca cctggctacc cgtcagctgg gcgcgtaaaa
    21901 aaggcggcct gcataccgtt gtcgatagca caacactttc acggccgctt tatgccatat
    21961 ggctgcaaaa tagcgataaa aatacgttga ttcgcgatct tttgaaaatt aacgtgctgg
    22021 atgaagtgta ttaatatgaa tggctggcaa ggatgccggt agaaggattt acttcggaga
    22081 gggttatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggcaggggcg
    22141 gagagactcg aactcccaac acccggtttt ggagaccggt gctctaccaa ttgaactacg
    22201 cccctaatta gggtggcgga acggacggga ctcgaacccg cgaccccctg cgtgacaggc
    22261 aggtattc
//
LOCUS       DAEVZA010000084        22219 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEVZA010000084 DAEVZA010000000
VERSION     DAEVZA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.084"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..2334)
                     /gene="acnB"
                     /locus_tag="LAF92_003157"
     CDS             complement(<1..2334)
                     /gene="acnB"
                     /locus_tag="LAF92_003157"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBI9792119.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQAR"
     gene            2689..4446
                     /locus_tag="LAF92_003158"
     CDS             2689..4446
                     /locus_tag="LAF92_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBI9792120.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPIITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(4517..5941)
                     /gene="lpdA"
                     /locus_tag="LAF92_003159"
     CDS             complement(4517..5941)
                     /gene="lpdA"
                     /locus_tag="LAF92_003159"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBI9792121.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(6266..8158)
                     /gene="aceF"
                     /locus_tag="LAF92_003160"
     CDS             complement(6266..8158)
                     /gene="aceF"
                     /locus_tag="LAF92_003160"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBI9792122.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(8173..10836)
                     /gene="aceE"
                     /locus_tag="LAF92_003161"
     CDS             complement(8173..10836)
                     /gene="aceE"
                     /locus_tag="LAF92_003161"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBI9792123.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(10997..11761)
                     /gene="pdhR"
                     /locus_tag="LAF92_003162"
     CDS             complement(10997..11761)
                     /gene="pdhR"
                     /locus_tag="LAF92_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBI9792124.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            12302..13675
                     /gene="aroP"
                     /locus_tag="LAF92_003163"
     CDS             12302..13675
                     /gene="aroP"
                     /locus_tag="LAF92_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBI9792125.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(13718..14572)
                     /gene="ampE"
                     /locus_tag="LAF92_003164"
     CDS             complement(13718..14572)
                     /gene="ampE"
                     /locus_tag="LAF92_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBI9792126.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(14569..15120)
                     /gene="ampD"
                     /locus_tag="LAF92_003165"
     CDS             complement(14569..15120)
                     /gene="ampD"
                     /locus_tag="LAF92_003165"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBI9792127.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            15208..16101
                     /gene="nadC"
                     /locus_tag="LAF92_003166"
     CDS             15208..16101
                     /gene="nadC"
                     /locus_tag="LAF92_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBI9792128.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            16304..16744
                     /gene="ppdD"
                     /locus_tag="LAF92_003167"
     CDS             16304..16744
                     /gene="ppdD"
                     /locus_tag="LAF92_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBI9792129.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            16754..18139
                     /gene="gspE"
                     /locus_tag="LAF92_003168"
     CDS             16754..18139
                     /gene="gspE"
                     /locus_tag="LAF92_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HBI9792130.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLARTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            18129..19331
                     /gene="hofC"
                     /locus_tag="LAF92_003169"
     CDS             18129..19331
                     /gene="hofC"
                     /locus_tag="LAF92_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBI9792131.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(19366..20409)
                     /gene="guaC"
                     /locus_tag="LAF92_003170"
     CDS             complement(19366..20409)
                     /gene="guaC"
                     /locus_tag="LAF92_003170"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBI9792132.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(20565..20609)
                     /locus_tag="LAF92_003171"
     CDS             complement(20565..20609)
                     /locus_tag="LAF92_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792133.1"
                     /translation="MIIKTLKMSARKRN"
     gene            20634..21254
                     /gene="coaE"
                     /locus_tag="LAF92_003172"
     CDS             20634..21254
                     /gene="coaE"
                     /locus_tag="LAF92_003172"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBI9792134.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            21254..21997
                     /gene="zapD"
                     /locus_tag="LAF92_003173"
     CDS             21254..21997
                     /gene="zapD"
                     /locus_tag="LAF92_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBI9792135.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            22007..22204
                     /gene="yacG"
                     /locus_tag="LAF92_003174"
     CDS             22007..22204
                     /gene="yacG"
                     /locus_tag="LAF92_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBI9792136.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT         5236 a         6025 c         5631 g         5327 t
ORIGIN      
        1 acgcgcctgg ttacccatac acagagaaca gcccgggatc tcgatacgag ccccgctctt
       61 accgaagacg ctgtagtagc cttcttcagt caactgtgcg gcgtccatac gggttggcgg
      121 tgccacccac aggcgggtcg gcaactggcc tttatgcgca tccagcagtt tacccgcagc
      181 acggaagtga ccgatgttag tcatgcaaga accgataaat acttcgtcga tcttctcgcc
      241 ctgtaccgca gacagcggac gcgcgtcatc cgggtcgttc ggtgcacaga ggattggctc
      301 tttaatatcc gccagatcga tgtcgatcac tgccgcgtat tcagcatctg catcggcttc
      361 cagcagctca ggattcgcca gccatttttc catgccctga atacgacgtt cgagggtacg
      421 acgatcgcca taaccttccg cgatcatcca cttcagcagg acgatgttag agttcaggta
      481 ttcgatgatc ggttctttgt tcagcttgat agtacaacca gcggcagaac gctcagcgga
      541 cgcatcggtc agttcaaatg cctgctcaac tttcagatcc ggcagacctt caatttccag
      601 aatgcgacca gagaagatgt ttttcttgcc tttcttctca acggtcagca gaccttgttt
      661 gatcgcatac agcgggatcg cgtgtaccag atcgcgcagg gtgatgcccg gctgcatttt
      721 gcctttgaag cgcaccagaa cggattccgg catatccagc ggcattacgc ctgttgcggc
      781 tgcaaatgcc accagaccag aacccgccgg gaaagagata ccgatcggga aacgggtatg
      841 ggagtcacca ccggtaccga cggtatccgg cagcagcata cggttcagcc aggagtggat
      901 tacgccgtca cccggacgca gcgacacacc accacggttc ataatgaagt ccggcagcgt
      961 gtggtgcgtg ttcacgtcaa ctggcttcgg atacgccgcg gtatggcaga aagactgcat
     1021 caccaggtca gccgagaagc ccaggcacgc caggtctttc agttcatcac gggtcatcgg
     1081 gccggtggtg tcctgagagc ctacagaagt cattttcggt tcgcagtacg cgcccggacg
     1141 aatgcctttc acgccacagg cacggcctac cattttctgc gccagcgaga agccgcgatc
     1201 gctctcagcg acatctttcg cctgacggaa cacatcactg tgcggcagac caagtgcttc
     1261 acgcgctttg gtggtcaggc cacgcccgat aatcagcgga atacgaccgc cagcacgcac
     1321 ttcatcaatc agcacgtcgg ttttcagttc gaaggtcgcc agcagttcgc cggtttcgtg
     1381 gttacgcact tcacctttgt acgggtaaac gtcaatcacg tcgcccatgt tcaggttaga
     1441 gacgtcgact tcgattggca gtgcacccgc gtcttccatc gtgttaaaga agatgggtgc
     1501 aattttaccg ccgaggcaca aaccaccgcc gcgtttgttc ggcacatgtg gaatatcatc
     1561 gcccataaac cacagaacgg agttagtggc ggatttacgc gaagaacccg tacccacaac
     1621 gtcaccgacg tacgccagcg ggaaaccttt ctgttgcaga gcttcgattt gcttgatcgg
     1681 accaacaaca cctggctggt ctggctcaat accttcacgg gcgtttttca gcatcgccag
     1741 cgcgtgcagt gggatatccg ggcgtgacca cgcatccggt gccggagaaa ggtcatcggt
     1801 gttagtttcg ccagtgactt tgaagacggt aacggtcagt ttttcagcca gcgccgggcg
     1861 attcaggaac cattcggcat ccgcccagga ctgcataacc tgcttcgcat attcgttgcc
     1921 tgctttcgct ttctcttcta cgtcatagaa gttatcgaac atcagcagcg tgtgagaaag
     1981 tgctttggca gcaataggtg ccagtttggc atcatccagc gcgtcgatca gcggatgaat
     2041 gttgtaacca ccctgcatgg tgcccagcag ttcgatggct ttttccggag tcagcagagg
     2101 ggatttggct tcgcctttcg cgatagcagc caggaagcct gctttgacat aggcggcttc
     2161 atcgacgcct gggggaacac ggttggttaa cagatctaac aggaattctt cttcgcccgc
     2221 gggcgggttt ttcagcagct ctacaagtgc ggccatttgg tttgcatcca ggggtttggg
     2281 cgcaatcccc tcagcggcac gctcagctac gtgcttacgg tattcttcta gcacgacggt
     2341 tctcctcgct ctcattgtca tagtgcggca ggcgattctc ttcacgctcc tgtgagacag
     2401 cagtttgtag ggtaaatgcc cagtaccgca acgggcagaa tagcaggatt ttgacgatgt
     2461 gttaatctgt ttacaaaaaa gcaacataaa aaagtggctg aatcgttaag gatggtctag
     2521 agattgtttc cctccgtaaa aacttcgtta attcgcatgg tgataatcac ctttctcaac
     2581 gaatcccaag attagtcaaa atttaaacta ccgcctcttt atactcggat tcacagcacc
     2641 tgcgggtggc agttcgccga ccattgcgat ttccttgaga tccgaattat gaaaatgact
     2701 ttgccgttta aaccccatgt gctggcgcta atttgcagtg ccgggctttg tgccgcctct
     2761 accgggctat atataaaaag ccgcacagtg gaagcgcctg tggaaccgca atcgacacaa
     2821 cagactgcgc ctgacatcac cgcagttacg cttcctgcaa cggtttccgc accgcccgta
     2881 acgcccgccg tcgtcaaatc cgcattcagc actgcacaaa tcgatcaatg ggttgcgcct
     2941 gtcgcgctgt accccgattc tctgctttca caagtgttaa tggcatcaac ctatccggca
     3001 aacgttgctc aagcagtgca atggtcgcac gataatccac ttaaacaagg cgatgctgct
     3061 attcaggcag tatctgacca gccgtgggac gccagcgtta aatcactggt ggcctttcca
     3121 caattgatgg cattgatggg cgaaaacccg caatgggtgc aaaacctggg cgatgctttt
     3181 ctggcgcagc cgcaggacgt gatggactcg gtacagcgat tgcggcaact ggcgcaacaa
     3241 accggctcgc tgaagtcatc aaccgaacag aaagttatta ccacaacgaa gaaagctgta
     3301 ccggtaacac agacagtcac ggctcccgtc ataccatcca ataccgtttc aactgccagc
     3361 cccatcatta cagagcctgc aacaaccgtc atttccattg agcccgccaa tcctgatgtg
     3421 gtctatattc ccaactacaa cccaaccgtg gtttacggga actgggccaa tactgcgtat
     3481 ccgccggttt atctgccacc accagccgga gaaccgttta ttgacagctt tgtgcgcgga
     3541 ttcggctata gcatgggtgt tgctaccacg tacgcactat tcagcagcat cgactgggac
     3601 gacgacgatc atgaccatca tcatcatgac gatgatgatt atcatcacca cgatggcggt
     3661 catcgtgacg gtaatggctg gcaacacaac ggcgacaaca tcaatatcga cgtcaacaat
     3721 ttcaaccgta tcaccggtga gcatcttact gataagaata tggcatggcg gcacaatcca
     3781 aactaccgta atggtgtgcc ctatcatgat caggatatgg caaagcggtt tcatcaaacc
     3841 gatgtcaacg gcggaatgag tgccacgcag ctacctgctc caacacgcga cagccagcgt
     3901 caggcggcag caagtcagtt tcagcaacga acacacgccg cccccgtcat tacacgagat
     3961 acccaacgtc aggcagcggc acagcggttt aatgaagctg aacactatgg gagctatgac
     4021 gacttccgcg acttcagccg tcgccaaccc ctgacccagc aacaaaagga cgccgctcgt
     4081 cagcgttatc agtccgcctc gcctgagcag cgccaggcgg ttcgcgagaa aatgcagact
     4141 aacccacaga accagcagcg aagagaggca gcgcgtgagc gtattcagtc tgcaactccc
     4201 gaacagcgcc aggtatttaa ggaaaaagta cagcagcgcc cactgaacca acagcaacgt
     4261 gataacgccc gccagcgtat acaatcggca tcacctgaac aacgtcaggt ttttcgggaa
     4321 aaagttcagg agagccgccc acaacgtcta aacgacagta accatactgt caggctgaat
     4381 aacgagcaac ggtcagcagt acgcgaacgt ctctctgagc gcggagcaag gcgacaggaa
     4441 aggtaaattg cagacgtaaa aaaagcggcg tggttagccg cttttttaat tgccggatgt
     4501 tccggcaaac gaacaattac ttcttcttcg ctttcgggtt cggcaggtcg gtaatgctac
     4561 cttcgaacac ttctgccgcc aggcccacag actcgtgcag agtcgggtgc gcgtggatgg
     4621 tcagtgcgat gtcttcagca tcacaaccca tttcgattgc caggccgatt tcacccagca
     4681 gctcgccgcc gttggtaccg acaatcgcac caccgatcac acggtgagat tctttgtcga
     4741 aaatcagctt ggtcataccg tctgcgcagt cggaagcgat agcacgacca gaagcagccc
     4801 acgggaaggt ggcggtttca tagctgatgc ctttctcttt cgcttctttc tcagtcagac
     4861 ctacccatgc aacttctggt tcggtatagg cgatggacgg gataactttc ggatcgaagt
     4921 agtgtttctt accggcgata acttcagcgg caacgtgacc ttcgtgaaca cctttgtgtg
     4981 ccagcatcgg ctgaccgacg atatcgccga tagcaaagat gtgcggtacg ttggtacgca
     5041 gctgtttgtc aacgcggatg aaaccacggt cgtccacttc cacgccagct ttgcctgcgt
     5101 cgaggttttt accgttcggc acacgaccaa tcgctaccag cacggcgtcg taacgctgcg
     5161 gttcagcggg tgcttttttg ccttccatcg tcacataaat accgtcttct ttcgcttcaa
     5221 cggcggtaac tttggtttcc agcatcaggt tgaatttctt gctgatacgc ttggtgaaga
     5281 ctttaacgat gtctttgtca gctgccggga taacctggtc gaacatttca accacgtcaa
     5341 tctgtgaacc cagcgcgtgg tatacggtgc ccatttccag accgatgata ccgccaccca
     5401 ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag tcccagatac
     5461 gcggatcttc atgcggaata aacggcagtt ggatcgggcg agaacccgct gcaatgatcg
     5521 cattgtcgaa gttgatcacg gttttgccgt tctcaccttc aacttccagg gtgttagccc
     5581 cggtgaattt acccagaccg ttgaccactt tgactttgcg gcctttcgcc ataccagcca
     5641 gaccaccggt cagctgattg atcactttct ctttccaggt acgaatcttg tcaatatcgg
     5701 ttttcggttc gccgaagacg ataccgtgtt cagccagcgc tttggcttct tcgataactt
     5761 ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa acaccgccaa
     5821 gggtgttgta acgttctacg attacggttt ccagacctaa atcagcgcaa cggaaggcag
     5881 cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt tcagtactca
     5941 tcatgacctc tatatattta tctccggcgg tcatacccgt cgtctttcag gccgcagcgg
     6001 cgttagcttc acgccctcac cccagtcact tacttatgta agctcctggg gattctggcg
     6061 cttgctacct tgctgcaccc agaaatccat agggtatgtg aagtaatacc ctaaccacca
     6121 ccgggtcgtt ctatccgtcg gtattttaca aaattgttaa caatttttaa acaacaaacg
     6181 gcaaccgatt tgtctattcg ctaataacct caatacattc atgagattac cagaaaaaag
     6241 ccggccgttg ggccggctct tttacttaca tcaccagacg gcgaatgtca gacagcgtgt
     6301 tgttaatgat ggtaatgaaa cgggcaccat cagcaccgtc gatcacgcgg tggtcgaagg
     6361 agagagaaat cggcagcatc agacgcggca cgaactcttt accattccac accggctcca
     6421 tcgcggactt ggaaacgccg aggatagcca cttccggcgc gttcacaatc ggcgcgaagt
     6481 gggtagtacc caggccgccg atgctggaga tggtgaagca accgccctgc atttcgcccg
     6541 cagtcagctt accgtcacgc gctttcttag aaatagtcat cagctcgcga gacagctcga
     6601 tgatgccttt cttgttgacg tctttgaata ccggaacaac cagaccgttc ggggtatcca
     6661 ccgccacacc gatgttgatg tatttcttca gggtcagacg ctgaccgtct tccgacaacg
     6721 aactgttgaa gcgaggcatc tgctcaagag ctgcagcaac ggctttcatg atgaagacaa
     6781 ccggggtgat cttcacatcc agcttacgtt tcgccgcttc ttcgttctgc tgtttacgga
     6841 acgcttccag ctcggtgata tcggttttgt cgaagtgagt aacatgcggg atcatcaccc
     6901 agttacggct caggttcgca ccagaaattt tctggatacg gcccagttcc acttcttcga
     6961 tttcaccaaa cttgctgaag tccaccttcg gccacggcag catgcctggg ataccgccgc
     7021 cagtcgccgc cggagctgct tctgcacgtt tgatagcttc tttcacgtaa gcctgaacgt
     7081 cttcgcgcag gatacgacct ttacggccag tgcccttcac tttcgccagg ttaacgccaa
     7141 actcgcgtgc cagacggcgg atcagcggag tcgcgtgaac gtaagcgtcg ttttctgcaa
     7201 attcagattt gccttccgct ttcgcagccg gtgctgctgc cggagcttca gcttttgctg
     7261 ccggagccgg cgctgccgct tcctgtttcg caggagctgc cgcaggcgct gcgccttcaa
     7321 cttcgaagat cataatcagc gagccagttt tcactttatc gccaacgttg actttcagtt
     7381 ccttcacgac gcctgcaaac ggagccggaa cttccataga agctttgtcg ccttctacgg
     7441 taatcagtga ctgttcagcg gcaactttgt cgcccacttt caccatcact tcggtcactt
     7501 caacttcgtc accgccgata tccggaacgt taacttcttt cacgccagcc gctggtgcag
     7561 gggccgctgc cggagccgct tcctgtttag ccgccggagc tgccgcgcct gcttcacccg
     7621 cgacttcgaa gaccataatc agcgaaccgg tagacacttt gtcacccacg ttcactttga
     7681 tctctttcac ggtgccagca aacggagccg gaacttccat agaagcttta tcgccttcta
     7741 cggtgatcag cgactgttca gcttcaactt tatcgccaac tttcaccagg atttcggtca
     7801 cttcaacttc gtcgctgccg atatccggaa cgttaacgtc ttttgccgct gcagccgctg
     7861 gtgctgctgc cggagctgct tctttcttct cttctgcctg agcaggtgca gcgtctgctg
     7921 caccgtcggc ggaatcgaaa atcataatca gtgcgccggt ctgggtttta tcgccaacag
     7981 agactttgat ctctttaacg atacccgcct gcggagacgg aacttccata gaggctttgt
     8041 cgccttctac ggtgatcagc gactgttcgg cttcaacttt gtcgcccact ttgaccagga
     8101 tctcggtgat ttcaacttca tcagccccga tgtccggtac tttgatttcg atagccatta
     8161 ttcttttacc tcttacgcca gacgcgggtt aactttatct gcatcgatgt tgaatttggc
     8221 gattgcgtca gcaaccactt tcttatcgat ttcgccacgt ttagccagtt cgcccagcgc
     8281 cgctacaacc acgtaggaag catcaacttc gaagtggtga cgcaggttct cacggctgtc
     8341 ggaacgaccg aagccatcag tacccagtac gcggtagtcg tcagccggta cgtaagtacg
     8401 gacctgctca gcgaacagtt tcatatagtc ggtagatgcc actgccggag cgtcgttcat
     8461 cacctgagcg atatacggaa cgcgcggagt ttccagcgga tgaagcatgt tccagcgttc
     8521 acaatcctga ccatcacgcg ccagctcggt gaaggaggtc acgctataaa cgtcagaacc
     8581 tacgccgtaa tctttcgcca ggatctctgc cgcttcacgg acgtgacgca ggatagaacc
     8641 ggagcccagc agctgaactt tacctttgct accttcaatg gtttcgagtt tgtagatacc
     8701 tttacggata ccttcctcag caccttccgg cattgccggc atgtggtagt tttcgttcag
     8761 cgtagtgatg tagtagtaaa cgttctcttg tttttcaccg tacatacgct ccagaccgtc
     8821 atgcatgatg acagcaactt cgtaagcgta agccgggtcg taagagatac agttcgggat
     8881 agtcagcgac tgaatgtggc tgtgaccatc ttcgtgctgc agaccttcac cgttcagggt
     8941 ggtacgaccg gaagtaccgc cgatcaggaa gccacgcgct tgctggtcgc cagccgccca
     9001 gcacagatcg ccaatacgct ggaagccgaa catcgagtaa tagatgtaga acgggatcat
     9061 cggcagattg ttggtgctgt aagaggtcgc cgctgccagc caggaacaac ctgcgcccag
     9121 ctcgttgatc ccttcctgca gaatctgacc tttctcgtct tctttatagt aagcaacctg
     9181 ctcgcggtcc tgcggggtgt actgctgacc gttcgggctg taaataccaa tctgacggaa
     9241 cagaccttcc ataccgaaag tacgcgcttc gtcggcgatg atcggtacca gacgatcttt
     9301 gatcgacttg ttcttcagca ttacgttcag ggcacgaacg aaagcgatag tggtagagat
     9361 ctctttgctc tgctcttcca gcagcgcgcc gaagtcttgc aggctcggca gctcaagctt
     9421 ctcggtgaag ttcggctgac ggcttggcag ataaccgtgc agtttctgac gctgtgcgtg
     9481 cagataggta tgctcttcag aaccttccgg gaaggtgatg tacggcagtt tttcgatatc
     9541 tgcatcagac accggcacat tgaaacggtc gcggatgtga cgcacgccgt ccatgttcat
     9601 tttcttaacc tggtgcgcga tgtttttacc ttcagccgcg tcgcccatgc cgtaaccttt
     9661 aatggtatga gcaaggatta ctgtcgcttt gcctttggtt tcctgcgctt tcttgaatgc
     9721 agcgtagatt ttcttcggat cgtgaccacc acggttcagt gcccagatct gctcgtcagt
     9781 ccagtctgca accagtgctg cggtttcagg atatttaccg aagaagtgtt cacgaacgta
     9841 cgcaccatct ttcgatttga aggtctggta gtcgccgtca acggtttcgt tcatcagctg
     9901 gatcagttta ccgctggtat ctttacgcag cagttcatcc caacggctac cccacatcac
     9961 tttgatcacg ttccagccag caccttcgaa gatgccttcc agttcgttga tgatcttgcc
    10021 gttaccggtg accgggccgt caagacgctg caggttacag ttgataacga agaccaggtt
    10081 atccagtttt tcacgggtag cgatggtgat cgcacctttg gattccggtt cgtccatttc
    10141 accgtcgccg aggaacgcgt aaacggtctg tttagaggta tctttcaggc cacggtgttc
    10201 cagatatttc aggaatttag cctggtaaat agcaccaatc ggccccagac ccatagatac
    10261 ggtcgggaac tgccagaatt ccggcatcag tttcgggtgc ggataggaag agaggccatt
    10321 gccgtgaact tcctgacgga agttatccag ctgctcctga gtcagacgac cttccaggaa
    10381 agcacgagcg tacacgcccg gggagatgtg gccctggaag taaaccaggt cgccgccatc
    10441 ctgctcgttg cgtgcacgga agaagtggtt aaagcacaca tcataaatgg ttgcggaaga
    10501 ctggaaggac gccatgtggc cgcccagttc gaggtctttt ttcgacgcac gcagcaccgt
    10561 catgatggcg ttccagcgga tagctgaacg aatacggcgt tccagttcca gattacccgg
    10621 atactccggt tgttcttcaa cggggatggt gttgatgtag ttgctgatac ctgtgcctgc
    10681 ggctacgttt acaccgcctt tgcgggcttc agcaagcagt tggtcgatca gatactgagc
    10741 acgctcaaca ccttcttcac ggatgaccga ttcgatcgcc tggagccagt cgcgagtttc
    10801 gatcggatcc acgtcatttg ggaaacgttc tgacatgggt tattccttat ctatctaata
    10861 acgttgagtt ttctggaacc tgtcccattg aactctcgcc ggaaagctca ataagacagg
    10921 ttctacgttt agttgccgcg cttttatatg cgcttgattt acaacatctt ctggataatt
    10981 tttaccagaa aaatcactaa ttctttcgtt gctccagacg acgcagagaa cgctcacggc
    11041 ggctctcttc acgacttctg tcgagcaaaa tttcttcgat aaaggccaga tggcgatgcg
    11101 atgcttcgcg cgcttcttcc ggcttaccgg ccataatcgc ttcaaatatg cgggtgcggt
    11161 gactactcac cagcggcagc atctcgcgac gcgaatagag caattcgaag ttctggcgga
    11221 cattctgggc caacatcggc tccatacacc ttagcagatg aagcagaacc acattgtggg
    11281 ccgcttcggt gacggcaatc tgatactgga gtacggcgtt tgattccgcg tccagatcgc
    11341 cagactgctg cgccagctct atggcgtggt ggagttcacg gatgcgttcc ttgtcttcat
    11401 cggtactacg cagcgcggcg taataagcgg cgataccttc cagggcgtgt cgtgtttcga
    11461 gcaagtcata ctgtgactca ggatggtcgg agagcagctc caccagcgga tcgctgaagc
    11521 tttgccatag gctgctctgg acaaaagtgc cgccaccctg gcgacgaagc aacaagccct
    11581 tcgcttcgag acgttgaatc gcctcacgca aggagggacg ggagacgtca aactgttttg
    11641 ccagttcgcg ttccggtggg agtttttcgc ccgggcggag agtgccttcg aggatcaaaa
    11701 actccagttg ctgctcaatc acatcggaga gttttggttg gcggattttg ctgtaggcca
    11761 tgagttcctg tcttaagcca cttgccgaag tcaattggtc ttaccaattt catgtctgtg
    11821 acgctaaagt aacaaagtat tcaccttatg tccatacagg ttttgattga aatcatgaaa
    11881 ctgtgcacat tttaacaact tgacatatat aacgtttcaa agttgtaact atgcacaaat
    11941 gtagacttta cgtaggaaag gagtttttta acgattatta aactataaaa atccgaattg
    12001 aaccgattca cttaccaatt ttgtgttttt taattcaatt aaaacgaatt taaattcatt
    12061 ctacatattg agaggggttg aggctgagct ttacaaacgg tttcttttta agcaactcat
    12121 cttcaaccat gcataaagcg ggtgcattcg ctgccgcata ccattattct tgatctgacg
    12181 gaagtctttt tgtaacaatt caaacttctt tgatgtaaac aaattaatac aacaaacgga
    12241 attgcaaact tacacacgca tcactgcgta gatcaaaaaa acaaccaccg cacgaggttt
    12301 catgatggaa ggtcaacagc acggcgagca gctaaagcgc ggccttaaaa accgccatat
    12361 tcagcttatc gcgctgggtg gcgcgatagg gaccgggtta ttcctgggta gcgcctccgt
    12421 aatacagtcc gcagggccag ggattatcct gggttacgcc attgctggtt ttatcgcctt
    12481 tctgatcatg cgtcagctgg gtgaaatggt ggtcgaagaa cctgtcgcag gctcctttag
    12541 ccactttgct tataaatact ggggcagttt tgccggtttc gcctctggct ggaactactg
    12601 ggtactgtac gttttagttg ccatggctga gctgactgcc gtgggtaaat acattcagtt
    12661 ctggtatccg gaaatcccca cctgggtttc tgccgccgta ttctttgtgg tgattaacgc
    12721 catcaacctg accaacgtta aagtgtttgg cgagatggag ttctggtttg ccattatcaa
    12781 agttatcgcg gtggtagcga tgatcatctt cggcggctgg ctgctattca gtggcaacgg
    12841 cggcccgcag gcgaccgtta gcaacctgtg ggatcagggt ggtttcctgc cgcacggctt
    12901 caccgggctg gtgatgatga tggcgattat catgttctcg ttcggtggtc tggaactggt
    12961 ggggatcacc gcagcagaag ctgataaccc ggagcaaagt ataccgaaag caactaacca
    13021 ggttatctac cgcatcctga ttttctatat tggttcgtta gccgttctgc tctcactgat
    13081 gccgtggacc cgcgttaccg ccgataccag tccgtttgtg ctgatcttcc acgagttagg
    13141 cgataccttt gtggcgaatg cgctgaacat cgtggtactg actgcggcgc tctccgtgta
    13201 caacagctgc gtatattgca acagccgtat gctgtttggt ctggcacaac agggtaatgc
    13261 gccaaaagcg ctggcgtctg tcgataaacg tggtgtacca gtaaatacca ttctggtgtc
    13321 tgcactggta acggcgttgt gcgtactgat taactacctt gccccagagt ccgctttcgg
    13381 actgttaatg gcgctggtgg tatctgcact ggtaatcaac tgggcgatga ttagcctggc
    13441 gcatatgaaa ttccgtcgcg ccaagcagga acaaggcgtg gtaactcgct tccctgctct
    13501 gctttatccg ctgggtaact ggatctgcct gctgtttatg gcggcggtac tggtgattat
    13561 gctgatgacc ccaggaatgg cgatttcggt atacctgatc ccggtatggc tgatcgtgtt
    13621 aggtatcggc tatctgttta aagagaaaac cgccaaagcc gtaaaagcgc attaatctct
    13681 ctacgccctc acccgtacag ggtgagggca ataatcttta caccaacgcc ccgtaaatcg
    13741 tcagtagtgc aatcaccacc acgaccacga acgaggtttt cttcgccatt gaaaccgctg
    13801 ccttcggcgt ctccacctta tcgacatgcg gttcacgcgc cagagagaac tgcgccagac
    13861 gcgttaacac ctgatactgc gaagtatgga aatcacccag cgaagcaaac caggccggta
    13921 acgctttctc accatgaccg atcaaggcat ataccacacc cgcaagacga accggcaccc
    13981 aatccagtac atgaagcacg gcatcaatgc cggactgtaa acgatgatgc ggcgtctgat
    14041 atcgcgccag ccagtattgc catgcacgca aaaacgcata ccccatcagc gtaacgggtc
    14101 cccaggttcc ccccacaatc agccagaaca gcggtgcaag ataaaaacga aagttaatcc
    14161 acagcaatgc attttgcagc tcacgcaaat actcacgttc gtcgcagcct gccgggacgc
    14221 cgtgaatcat ggtgagttcg ccagccatcg tggcacgggc atggctatca ttacgtgaag
    14281 cagctgtcag ataagcatga taatgaagac gaactttacc tgcgccaata cacagcaaac
    14341 caatcagcag ccacaccagt agcgtgggaa cgttgaacaa tactccctgc aatgcgcgta
    14401 acagtaaaaa agtcacgccc atcgcaataa tggtcatgcc taacgtgcgc ccgagagaaa
    14461 aatgtttcac ccgccgaaag aacgcttcaa gacgatgatc aagctgccag tgctcgccca
    14521 acttaaacag gcgctcgaaa attaacacca gtaaggttgt aaatagcgtc atgttgtctc
    14581 cttgctgacc agcacacgaa accgtgccca atcaaatgca ggaccgggat cggttttccg
    14641 atccggcgca atatcacaat ggcccgtcat gtttttagcg atatccggat agcaatcaat
    14701 cagtgcccgc gtaaccgccg caagctgttg atactgcgca tcggtatacg ccagcgtatc
    14761 ggtgccttca agctcaatcc caatagaaaa atcattgcag cgttcgcgcc cctgatactg
    14821 agagactccc gcatgccatg cacgtttatc gaaaggaaca tactggacta tttcaccatc
    14881 acggcgaatc aaacagtgag cggagacgcg caaatgggcg atctcagcaa agaaaggatg
    14941 tgcctgcgga tcaatagttc cagtgaataa tgcgtcgatc cacggaccgc caaactcgcc
    15001 tggcggcagg ctaatattgt gcaccaccag cagggtgggt gtttcgtcat ccgggcggca
    15061 atcgtaatgt ggtgagggaa cgcggcgcgc gccaaccagc cacccctgtt ctaacaacat
    15121 gcaggatctc cttatatgtg gtgctaatac ccggttcaga gtagcatgtt tctaccttat
    15181 gattcgttag ctatctggag ttttaacatg ccgcctcgcc gctataaccc tgacacccga
    15241 cgtgacgagc tgctggaacg cattaatctc gatatccccg gcgcggtggc ccaggcgctg
    15301 cgggaagatt taggcggaac agtcgatgcc aacaatgata ttacggcaaa acttttaccg
    15361 gaaaattctc gctctcatgc cacggtgatc acccgcgaga atggcgtctt ttgcggcaaa
    15421 cgctgggttg aagaggtgtt tattcaactg gcaggcgacg atgtcaccat aatctggcat
    15481 gtggatgacg gcgatgtcat caatgccaat caatccttgt tcgaacttga aggcccatcc
    15541 cgcgtgctgt taacgggcga acgcactgcg cttaattttg tgcaaaccct ttcaggagtt
    15601 gccagtaagg tacgccacta tgtcgaattg ctggaaggca ccaacacgca gttgttggat
    15661 acgcgcaaaa ccttacccgg cctgcgttca gctctgaaat acgcggtact ttgcggcggc
    15721 ggagcgaatc accgtctggg gctttctgat gccttcctga tcaaagaaaa ccatattatt
    15781 gcctccggct cagtgcgcca ggcggtcgaa aaagcgtcct ggctgcaccc ggatgcgcca
    15841 gtagaagtcg aagtagagaa tctggaagaa cttgatgaag ccctgaaagc aggagccgat
    15901 atcatcatgc tggataactt cgaaacagaa cagatgcgcg aagccgtcaa acgcaccaac
    15961 ggcaaggcgc tactggaagt gtctggcaac gtcactgaca aaacactgcg tgaatttgcc
    16021 gaaacgggcg tggactttat ctccgtcggt gcgctaacta aacacgtaca agcactcgac
    16081 ctttcaatgc gttttcgcta atagttgaca gatttatccg ctccatcgcg ggcggataat
    16141 ctccccttcc ccaactttct tcgtaacgcc tcgcaaattt cttgtctttc gctgcaacga
    16201 aataaaaaac ctcggaatct gctttcccgt ttcgtttttt gcccctcgcc tgtgctgatg
    16261 gcgtttgcca aagtagcgcc aaccaaatca aggagcgaaa cagatggaca agcaacgcgg
    16321 ttttacactt atcgaactga tggtggttat tggcatcatt gccattttaa gcgccattgg
    16381 tattcccgct tatcaaaact acctgcgcaa agccgcactc accgacatgc tacaaacctt
    16441 tgtgccttac cgtaccgccg tagagttgtg cgcgctggaa catggtggat tagatacctg
    16501 cgacggtggc agcaatggca ttccctcgcc taccaccacc cactatgttt cagccatgag
    16561 tgtggcaaaa ggcgtggtgt cgctgaccgg gcaagaaagt ctcaatggac taagcgtcgt
    16621 catgacaccg ggttgggata acgcaaacgg cgtcaccggc tggacgcgca actgcaatat
    16681 tcaaagtgac agcgcattgc agcaagcctg cgaagatgtc ttccgctttg atgacgccaa
    16741 ctaaggagcg gcaatgaata ttccacagct cacggccctg tgtctgcgtt atcagggagt
    16801 cttgctggat gccagcgaag aagtggttca tgttgcggtg gtcgatgccc cctcacatga
    16861 gttgctggac gcattgcatt tcgctaccac caaacgtatt gagatcacct gctggacgcg
    16921 ccaacaaatg gaaggtcacg ccagtcgcac acaacagaca ttgcccgtag ctgttcagga
    16981 gaagcatcag cccaaagcag agttgctagc tcgaacgtta caatctgcgc tggaacaacg
    17041 cgcgtctgat attcatatcg aaccagcgga caatgcctac cgcatccgct tgcgtatcga
    17101 cggcgtattg catcctttac cggatgtttc accggatgcc ggagtcgcat taaccgccag
    17161 attaaaagtg ctgggaaacc tggatattgc ggaacatcgc ctgccgcagg acgggcaatt
    17221 cactgtcgaa ctggcaggaa acgccgtctc atttcgtatt gcgaccttac catgtcgggg
    17281 tggtgaaaag gtggtattaa ggttgttaca gcaggtgggt caggcactgg atgtcaacac
    17341 gcttggaatg cagccgttac aactggcgga ctttgctcat gccttgcaac aaccacaggg
    17401 actggtgctg gtaactggcc ctacaggcag cggcaaaacg gtcacgcttt atagtgccct
    17461 gcaaacgctg aataccgctg acattaatat ttgtagcgtc gaagatccgg ttgagatccc
    17521 catagccgga ctaaaccaga cgcaaatcca tccgcgtgcc ggactcacct ttcagggcgt
    17581 gttgcgtgcg ttattgcgcc aggatcctga cgtcatcatg atcggagaga tccgcgatgg
    17641 cgaaacagca gagatcgcta ttaaagcggc gcaaactggt cacctggtgt tgtctaccct
    17701 acacactaat tccacctgcg aaacgctggt acgtttacag caaatgggag tcgcccgctg
    17761 gatgctctca tcagcgctta cgctggtaat agcccagcgt ctggtacgta aactttgccc
    17821 acattgtcgc cagcagcaag gggagcccat ccatattcca gtcaatgtat ggccgtcgcc
    17881 gctgccccac tggcaggcac ccggttgtgt acattgctac cacggttttt atggtcgtac
    17941 ggccttattt gaagttctgc ccataacgcc ggtcattcgt cagcttattt ccgctaatac
    18001 cgacgttgaa tcgctggaaa cgcacgcacg acaggcgggt atgtgtacgc tttttgaaaa
    18061 cggctgcctg gccgtggagc aaggcttaac cacctttgaa gagttaatcc gcgtactggg
    18121 gatgccgcat ggcgagtaag caactctggc gctggcatgg cataaccggc gacggcaatg
    18181 cgcaagatgg gatgctatgg gcagagagcc gtgctttgct gctcatggca ctacagcaac
    18241 agatggttac cccacttagc ctgaagcgaa tcgccatcaa ttctgcgcag tggcgaggag
    18301 ataaaagcgc ggaagtcatt catcaactgg cgacgctact caaagccggg ttaacgcttt
    18361 ctgaagggct ggcactgctg gcggaacagc atcccagtaa gcaatggcaa gcgttgctgc
    18421 aatcgctggc gcacgatctc gaacagggca ttgctttttc caatgcctta ttaccctggt
    18481 cagaggtatt tccgccactc tatcaggcga tgatccgcac gggtgaactg accggtaagc
    18541 tggatgaatg ctgctttgaa ctggcgcgtc agcaaaaagc ccagcgtcag ttgaccgaca
    18601 aagtgaaatc agcgttacgt tatcccatca tcattttagc gatggcaatc atggtggttg
    18661 tggcaatgct gcattttgtt ctgccggagt ttgccgctat ctataagacc ttcaacaccc
    18721 cactaccggc actaacgcag gggatcatga cgctggcaga ctttagtggc gaatggagct
    18781 ggctgctggt gttgttcggc tttctgctgg cgatagccaa taagttgctg atgcgccgac
    18841 cgacctggct tatagtgcgg cagaaattgc tgttacgcat cccgattatg ggttcactga
    18901 tgcggggaca aaaactcacg cagattttta cgattctggc gctgacacaa agtgcaggca
    18961 ttactttttt gcagggcgta gagagcgtca gagaaacaat gcgctgcccg tactgggtgc
    19021 aacttctgac acaaatccag cacgatatca gtaacggtca tcccatctgg ctggcgctaa
    19081 aaaataccgg ggagtttagc ccgctctgtt tgcaattagt gagaacagga gaggcatccg
    19141 gctcgctgga cctcatgtta gacaacctcg cccatcatca tcgggataac acaatggcgc
    19201 tggcggataa cctcgcagcc ttactggaac cggcgttgct gatcataacg ggaggaatta
    19261 tcggtacgct ggtggtggcg atgtatctgc caattttcca tttaggcgat gcgatgagtg
    19321 ggatgggata acgctggcgt gttgctccac accagcgttg gaagattaca ggttgttgaa
    19381 gatgcggttt tcttgttcct gcacacggat aaatgtggtg cgcttggtca gttctttcag
    19441 gcgtgaagcc ccaacgtatg tacaagctga acgcaggccg cccaaaatat cacgcgcggt
    19501 attttcaacc gggcctcgca gcggcagctt aacggtttta ccttctgctg cgcgatattc
    19561 cgcaacgccg ccaacgtgac gtttcatcgc agactcggag ctcatgccgt agaacagcat
    19621 aaatttctcg ccgttctcct caacgatgcg accgccgctc tcttcgtggc ccgccagcat
    19681 gccgccaagc atgacgaaat cggcaccgcc gccaaaggct tttgcgacat cgcccggcgt
    19741 ggtgcagcca ccgtcgctga cgatcattcc gcccagaccg tgcgccgcat cggcacattc
    19801 gattaccgca gaaagctgcg gataaccgac gcctgttttg acgcgagttg tacaaacaga
    19861 acctgggcca atgccaactt taacgatatc agcaccggag aggataagct cctcacacat
    19921 ttcaccagtc actacgttgc cagcacaaat ggttttggtc ggccacgctt cacgcgcttt
    19981 cgcaacgaac tgcacgaagt gttcggaata accattcgcc acatcaatac aaacgaagtt
    20041 taatgccggg ttcaggtcga gaatctgttt agttttttcg aaatccgcat cagacgtacc
    20101 ggtagaaacc atcacatgtt tcagcacatc agcggaagaa ttgttgataa acgcttgcca
    20161 ctcttcgaca gaatagtgtt tatgcacagc agtcaaaata tcaaaagaag ccagcgcaga
    20221 ggccatagaa aatgtaccta cggtgtccat atttgcggcg ataattggca cgccggacca
    20281 gctctgacct gaatgtttga aggtgaattg acgttccagt tcaacatcgg aacggctttt
    20341 aagagtggag cgtttagggc ggatgagaac gtctttaaaa cctaacttca gatcttcttc
    20401 aatacgcatg tgcggattcc tggggttaat ggctataagg gtaagagcgc aactccagtg
    20461 acgctatcat acgcactaat caatgcggcg caagactgcg aaattgtctt tttttacgct
    20521 acaatcccat aaatttacct ggtaaatagt gagcgaacgt ttcatcaatt tcgctttcgt
    20581 gcgctcattt ttaacgtttt gataatcatg atgaattccc gggaatataa attatgaggt
    20641 atatagttgc cttaacggga ggcattggca gtggcaagag taccgttgcc aatgcgtttg
    20701 ctgatctcgg aattaacgtc attgatgccg atattattgc gcgtcaggtg gttgaaccag
    20761 gtgcacctgc gctacatgcc attgctgatc actttggcgc taacatgatt gctgctgatg
    20821 gaacattgca gcgccgggcc ttgcgcgagc ggatcttcgc caacccggaa gagaaaaact
    20881 ggcttaacga cctgctgcat ccgctgattc agcaagagac gcaacaccag atccagcaag
    20941 caacttcccc ctatgtactg tgggttgtgc cattgctggt agaaaactca ctgtataaaa
    21001 aagcgaatcg agtgctggtg gtggatgtca gcccagaaac gcaacttaag cgcaccatgc
    21061 agcgcgatga tgtaactcgc gaacatgtcg aacaaatcct tgctgctcag gcaacgcgcg
    21121 aagcccgcct tgccgtggca gatgacgtca ttgataataa cggcgcaccg gatgctatcg
    21181 catcggatgt tgcccgcctg cacgcacact atttgcagct tgcgtcgcag tttgtctcac
    21241 aggaaaaacc gtaatgcaga cccaggtcct ttttgaacat ccactaaatg aaaaaatgcg
    21301 tacatggctg cgcattgagt ttttgattca gcaactcacc gttaatttac ccatcgttga
    21361 ccacgctggc gcgctgcatt tcttccgtaa tgtcagtgaa ttactggatg ttttcgagcg
    21421 cggcgaagtc cgcactgagc tgttgaaaga acttgaccgg cagcaacgta aactccagac
    21481 ctggattggc gtgcctggcg tggaccagag ccgtattgaa gcattaattc agcagttaaa
    21541 agcggcgggg agcgtattaa tttccgcgcc gcgtatcggg caatttctgc gtgaagatcg
    21601 tttgattgct ctggtgcgtc agcgactgag catcccaggc ggctgttgca gctttgattt
    21661 acctacattg cacatttggc tgcatctacc ccaggcgcag cgcgacagcc aggtagaaac
    21721 ctggattgcc agcctgaacc cgctcaccca ggcacttacc atggtgctgg atttaattcg
    21781 ccagtcggcc cccttccgta aacaaaccag cctgaatggt ttttatcagg ataacggtgg
    21841 cgatgccgac ttgctgcgcc tgaatctgtc gctcgattca cagctttatc cgcaaatttc
    21901 cggacataag agccgttttg ccattcgttt tatgccgctg gacactgaaa acggacaggt
    21961 accggaacgt ctggatttcg aactggcctg ttgctaagga gtgaaaatgt cagaaactat
    22021 tacggtgaat tgcccaacct gcgggaaaac ggtggtgtgg ggtgaaatca gcccgtttcg
    22081 gccattttgc tccaaacgtt gtcagctgat cgacctcgga gaatgggctg ctgaagaaaa
    22141 acgaatcccc agcagtggcg atctttccga aagcgatgac tggagcgaag agccaaagca
    22201 gtgacatttg tctgatgcc
//
LOCUS       DAEVZA010000085        21671 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEVZA010000085 DAEVZA010000000
VERSION     DAEVZA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.085"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..350)
                     /locus_tag="LAF92_003175"
     CDS             complement(<1..350)
                     /locus_tag="LAF92_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBI9792137.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNL"
     gene            complement(445..1359)
                     /gene="ldcA"
                     /locus_tag="LAF92_003176"
     CDS             complement(445..1359)
                     /gene="ldcA"
                     /locus_tag="LAF92_003176"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBI9792138.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            1459..2070
                     /gene="emtA"
                     /locus_tag="LAF92_003177"
     CDS             1459..2070
                     /gene="emtA"
                     /locus_tag="LAF92_003177"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBI9792139.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(2072..2806)
                     /locus_tag="LAF92_003178"
     CDS             complement(2072..2806)
                     /locus_tag="LAF92_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBI9792140.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            3007..3261
                     /locus_tag="LAF92_003179"
     CDS             3007..3261
                     /locus_tag="LAF92_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBI9792141.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            3439..3879
                     /locus_tag="LAF92_003180"
     CDS             3439..3879
                     /locus_tag="LAF92_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792142.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRYKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(3958..5655)
                     /gene="treA"
                     /locus_tag="LAF92_003181"
     CDS             complement(3958..5655)
                     /gene="treA"
                     /locus_tag="LAF92_003181"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBI9792143.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(5975..7393)
                     /gene="dhaM"
                     /locus_tag="LAF92_003182"
     CDS             complement(5975..7393)
                     /gene="dhaM"
                     /locus_tag="LAF92_003182"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBI9792144.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKSEQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETKEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(7404..8036)
                     /gene="dhaL"
                     /locus_tag="LAF92_003183"
     CDS             complement(7404..8036)
                     /gene="dhaL"
                     /locus_tag="LAF92_003183"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBI9792145.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(8047..9117)
                     /gene="dhaK"
                     /locus_tag="LAF92_003184"
     CDS             complement(8047..9117)
                     /gene="dhaK"
                     /locus_tag="LAF92_003184"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBI9792146.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            9345..11264
                     /gene="dhaR"
                     /locus_tag="LAF92_003185"
     CDS             9345..11264
                     /gene="dhaR"
                     /locus_tag="LAF92_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBI9792147.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(11364..14231)
                     /locus_tag="LAF92_003186"
     CDS             complement(11364..14231)
                     /locus_tag="LAF92_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI9792148.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(15000..16091)
                     /gene="ychF"
                     /locus_tag="LAF92_003187"
     CDS             complement(15000..16091)
                     /gene="ychF"
                     /locus_tag="LAF92_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBI9792149.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKV
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(16208..16792)
                     /gene="pth"
                     /locus_tag="LAF92_003188"
     CDS             complement(16208..16792)
                     /gene="pth"
                     /locus_tag="LAF92_003188"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBI9792150.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            17070..17348
                     /gene="ychH"
                     /locus_tag="LAF92_003189"
     CDS             17070..17348
                     /gene="ychH"
                     /locus_tag="LAF92_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBI9792151.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(17403..19082)
                     /gene="dauA"
                     /locus_tag="LAF92_003190"
     CDS             complement(17403..19082)
                     /gene="dauA"
                     /locus_tag="LAF92_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBI9792152.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(19207..20154)
                     /gene="prs"
                     /locus_tag="LAF92_003191"
     CDS             complement(19207..20154)
                     /gene="prs"
                     /locus_tag="LAF92_003191"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBI9792153.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(20305..21156)
                     /gene="ispE"
                     /locus_tag="LAF92_003192"
     CDS             complement(20305..21156)
                     /gene="ispE"
                     /locus_tag="LAF92_003192"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBI9792154.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(21156..>21671)
                     /gene="lolB"
                     /locus_tag="LAF92_003193"
     CDS             complement(21156..>21671)
                     /gene="lolB"
                     /locus_tag="LAF92_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBI9792155.1"
                     /translation="PQWRQHQQDVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDR
                     YRLLLTNPLGSTELELNAQPGNVQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQW
                     ILGLPGDATDYKLDDQYRLSEITYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQR
                     IKLKMDNWIVK"
BASE COUNT         5368 a         5411 c         5439 g         5453 t
ORIGIN      
        1 agattaaaca gccacgccgc catcatgccg gttaaaccag aggtgataag cacgcccagc
       61 gtcgccagcg acagtgccgg tcctaacgcc acacgaaagg aactggcctg agtgcgcatc
      121 ccgccgtcga gcaaaataat cgccagtgcc aggttactta ccatgtaggc gaaggggtaa
      181 ttatcaaacg ggataccgcc gacgccatcg actcctgcca gcatgccgat cgccagaaaa
      241 ataaccagaa taggaatgcc aagacgggaa gaaaatgaac taagtaatat actgctggtg
      301 acaaggatgg atcctaagat gaaaaggcta attattgttg tggcatccaa cggtcggtta
      361 ctcctgatta cgctgtctct tatataaacc ctaccatatt agcggcagag acagcgtttt
      421 acttagccct gaagcgtgat ttttttacat tttaagaaca ggatgaccgc taatcgtcaa
      481 ctgagtgcct tcccgggtgt tattcagaat ggcatgtgcg cccaacggca gcgtaaccgt
      541 gcgttgttca tgaccaaaat cgaggccggt aatgagtgga atcgacaggc gggaacgcaa
      601 aaacgcgtac actgactcca ggttgtaacc tgcgtcataa tcattgggcg tgctgccgct
      661 aaagctaccg agaataatcg ccttctgacg tggcaaaatt cccgcatgat agagctgcaa
      721 cagcatacgt tcgacgcgga aagggtgctc gttaatatct tccagtacca ggataccgtt
      781 ctcaattttt ggcatccacg gtgtaccaat cagtgaaatc agcatcgcaa gattgcctcc
      841 ccacaacgtg ccttcggtct gacatgtcgg gccttcccct tgccattcaa tggtgaaggt
      901 tttattgcgt aacgccagcc agaaatggtg ctcggtaaag gcgttcagtt catccgcacc
      961 aaagtttgct accagcatgg ggccgctaaa ggtgatgaca ttgccttgcg ccagaagacc
     1021 gcactgaatg gcggtaaaat cgctatgtcc gcaaatgagt aacgggtcat gttgttggcg
     1081 agccaccagt gcctgccagt caatatctgc cagtaaacga ctggcaccgt aaccgccgcg
     1141 tacagccagc acgatggtgt tgggggtagt cagtctggca agggaattga gatcttccag
     1201 gcgctccgtt tccgtacccg caaaacgctc acaacgacgg gcaataacct cgacgttatt
     1261 gacctgatgc ccctcgtcgg taaggcgatg gataccacgc agcgcggcgt gctgtttaat
     1321 gcagtaaccc gatggggcaa ttaagtgaaa cagagacatg gcaattcctt gctgacaaca
     1381 gaaacgaaat gtatatcatg ccgcttaggt gtgccgttgt cacctcaacg gcgattccag
     1441 gctataagga tagaagaagt gaaattgaga tggtttgcct ttttgattgt gttattagcg
     1501 ggttgttcat caaagcatga ctatacgaac ccgccgtgga acgcgaaagt tccggtgcaa
     1561 cgtgcgatgc agtggatgcc aataagccag aaagccggtg cagcctgggg cgtcgatcca
     1621 caattgatca cggcgattat cgctatcgaa tcgggtggta atccgaacgc ggtgagtaaa
     1681 tcgaatgcca ttggtttgat gcagttaaaa gcttcaacct ccggacgtga tgtttatcgc
     1741 cgtatgggct ggagtggtga gccgacgacc agcgagctga aaaatccaga gcgtaatatt
     1801 tcaatggggg cggcttacct gaatattctg gaaaccggcc cgctggcagg cattgaagat
     1861 ccgaaggtac tgcaatatgc gctggtggtg tcatacgcta acggggcagg tgcgctgcta
     1921 cggactttct cgtcagatcg gaaaaaggcg atcagcaaaa tcaacgattt ggatgctgac
     1981 gagttcctcg atcacgtagc gcgaaatcat cctgcgccgc aggctccgcg ctatatctac
     2041 aaacttgagc aggcactgga cgcgatgtaa atcagtcgcg cactttgtcc gctttttccc
     2101 gggcttctcg ctcgagagag aaaataatcc gctgtaattg ccgctccacc gtcgggctga
     2161 cgttaagaaa acggaagctc agacggggag tggtgatggt ttcattcttg ccatcaatca
     2221 ctttgcgctc gctgatggag attaactggg cgtcaaagtg aaaaacaccc cattgcccca
     2281 tgttgacttc aatctgagcg aagcgcatgc cttcttgtaa ttcggcaggc tttgctgttt
     2341 ccagtaatgc gcccatgccg cctaacgaca aatcatacag gcggaaacgt aacgtactgt
     2401 tatccgccag tttggtctgg caaaaataag gcggatggag tggggcggag atgcggaaat
     2461 atcggcgtcg ttgtacaaac cataaggtgg gaggcggtac ggtgataaat gccggaagct
     2521 gcaagtattc actctgctgt agttgttcaa cagtaaactc gactttcgca ccctgagttt
     2581 cggcggtaat ggtaatgtgc tgtgccttta gcacggcgat gttgtcttcg gcttgactgc
     2641 cgaaatccag caccagttta tccggggtta ttgccagtat tttgctgatc agctgcccgc
     2701 cattccaact gagacgcaaa ggaattgcgg ctttgtgcaa atcgcgtaac acgcccagga
     2761 cggctaacgg attttgtttc aggaactgct catggtaatg actcacgcgg aaaaactcct
     2821 gactgataaa ctgtctttgg tttatcggtc acagttaaca aaacttaata caaatgcgtg
     2881 aatatttttt acatgttgtt cttaaatcag ccgacatacg cccagcatcg atcccgccct
     2941 tgcctatact tagagcgttg acgtaagcat ttcttgcgtc cgattcatcg aaacgagggc
     3001 ctgaacatgg gaattattgc ctggattatt tttggcctga tagccggcat tatcgccaag
     3061 ctaatcatgc cggggcgtga tggtggtgga tttttcctga cctgtattct cgggatagtc
     3121 ggtgcggtgg tcggcggctg gctggcgacc atgtttggca ttggcggctc catcagtggt
     3181 tttaatctgc acagcttcct ggtggcggtg gtgggagcta ttctcgttct gggcgtattc
     3241 cgcctcctgc gaagagaata agattttcat aaggcggata gcgatacaga tgccgctatc
     3301 cgctttcaca tcagaacgta tactcgacac ctgctttacg ggtggaacaa atcaatatag
     3361 cacttagatg cccgataaag tcggttcccg gtaacaacaa cagaatcctt ttttaattat
     3421 tgtttcgttg tttttggtgt gattcagaaa ataaatattt tagacagtag ccagtcaggc
     3481 gttaaaaatg gggcggatag tgaggcggtt ttggctgcgc ttattgaaca tttagaattg
     3541 atcaatcctt ctggcagatt aagccaaaat acccgttcgg caatgcttca gttacgcgaa
     3601 gaggaatggt ctgaattctt tttttggttg ctaaattctc tggagtgtct ggattatgtg
     3661 attattaatt taactccaga aagcaaaaaa acgttgatga gtgaacaccg taacaatatt
     3721 caggtagcaa tcgatgcttt atataggcaa cgtcggtata aatcaccggg ggatgatagt
     3781 gaaacactta cccgtagaaa tgacgctatc ttcggcaacc acgtatggca aacatttgcg
     3841 caatacttcc cgccaggact tgaaaaaccg tctgtgtaga aaacaccata gctatggtgt
     3901 tatctgccga caacgccatc cggagaagcc gaatgacgtt gtcggagtaa gcgctggtta
     3961 aggtgtgggt tgtgcctctt tggttgaggg ttgcgtcgtt gctgacttaa cggtcggacg
     4021 cgtcgccgga acattgtcac acggttgctc tttcgggcag atcaaatcca gcattttcag
     4081 cgtcacgcca ttggtccagc caaagccatc ctgtaatgga tattcgccac cgccgccccc
     4141 cgttccggtg gtgctgacat catatttttc caccagcttt ttctcccggt cataggtgtg
     4201 ctgaacattg gtcaggaagt gccagctaat gtccatcgcc acctcttttt gcccgtagtt
     4261 ttgtaatcct tctgtcgcga cccactgtaa cggtgcccag ccatttggcg catcccattg
     4321 ttgcccactt ttcaccgacg tggtgttcag gccgccgggt tgcagcagat gtgttttcgt
     4381 cgccgtcgcc attttgttgg cgcgatcttt cgctgccgca ttgacgtaca gcgggaacag
     4441 ggcggccgcg gttaactgat tgcgcacttt atgacttttc aggtcgtaat cggcatacca
     4501 gccttgttga tcgttccaca ggtatttttc gatccctttt tgacgggcat ttgccagcgt
     4561 ttcgtactgg tttgccatcg cgttatctcc ggcagctttg ctggcgcggg cgaggatttt
     4621 ttccatttta aacatcaggc tgttcagatc gaccggtacg atgctggtgg tgcgtaaggt
     4681 atttaactgc tgcgggttgt ccatccagcg cgagctgaaa tcccagccag acgcagcggc
     4741 agagcgcagg tcgcggtaaa tttcagtggc aggtcgattc ggattgcttt tggcggtggc
     4801 aatatcttcc acccatgact ctggtcgtgg cgtatcgcga tcgtcccagt agcggttgag
     4861 aagggtacca tcctgaagtt tgacaacgcg tttttcctgt tgtccggctt gcaggttttc
     4921 aacaccgtcc atccagtaag catattcttt ttgcatttgc ggcaggtatt gcttcaacgc
     4981 ggcatcgcct tcatgctgcg ccagtaactc taccatcagg gcaaagaagg gcggttgcga
     5041 gcggcttaaa tagtaactgc ggttgccgtt gggaatatga ccgtaagtgt ctatttcatg
     5101 agcaaaattg gccaccatat ccgcgacttt atcccagtga ccgctttcgg caagtcctaa
     5161 catggtgaag taactgtccc agtaatatac ctcgcgaaaa cgtccgcccg gcacgacata
     5221 aggttccggc agcggtaaca gagaatccca tttttctgtg ttttcggtag aacgcgttaa
     5281 taccggccaa agtccgtcaa tatgttcgcg cagtgactgc ccctctggcg gaacatattt
     5341 ctcgccttct ttcggcaggg tgaaattgac gttaacgaaa tggcgcagat caaatccgct
     5401 ctggttttgc tgcatccgat aatcagcaag gatcatcagc ggatcgctgt tcggcacggc
     5461 atcggcaaag gttttttggt ccggaaaaag tttggcgttt tgcacatcat taaacagcgg
     5521 ccctaataaa atatcaggcg gctgtggtgt taccggtgtt tcttctgcct gcaccgatag
     5581 cgcagcgaaa cacaaaaaga tacaggctgg aattaacgcc attttttgcg ggcgagaagg
     5641 tgcgggggat ttcatcaatc attctccttt ggcgaaaccg aataaaacgg tattcaagta
     5701 tcagaaaacc ttagttcagg atcgcactag ctgcatgatc gaaatctcat tctgcgaact
     5761 gtcaggcatt ttagaaaagg gaattaaagg gataaataac ggcgaaatag agtgatcaaa
     5821 taacgtctgt ttattagcca gccagcggcg ttgggttcgt gattagcgaa gaaaaatcat
     5881 cataaattgg tgaaaaatat aacaggtagt taaaaaccat tagtgctgag taaattgccg
     5941 gatgacatca gaacgatgcc atccgaacag tggcttaacc ctgacggttg aaacgttgcg
     6001 ttttaacgtc cagcgttagc gtttcttctg gttgtatcgc atacagtttc tcaccctgct
     6061 ggcaaatcca gccaatcccc agctcacggg cgattagggc gctgtgggat accggacttc
     6121 cggcgctaag gcagatacct tttacaaccg ccggatccag ttgcagtact gtggaaggat
     6181 aaatgttctc cgccagtaga atagttggcg agttaaactg cgggagttct tctttcgttt
     6241 gggtcaggtg gaccagggtg cgatgcagaa gatcgtccac atcaatatag cgagcttgta
     6301 gatattcatc atccagttgc tggtattgct ggctaagttc tttaagaact tgctgccagg
     6361 catattctgc cgtgcaatgt tcatgctgaa ggagttcgct tgccgccgcc agcagttccg
     6421 gatcatctaa cagtgtatgg tgaccagaaa agattgcggc aatatcgtca agcccgctgg
     6481 cttctgcttt cgctgttaac gtcatcagat ctaataacgt gaagtcaata gcctggcgta
     6541 atcgatcttg ttcttcttcc acggtcaggg ttgattttgc ctgtaccgta cataaaactg
     6601 gttgataata aaaggcttta cccgaaacag gcggaacggg acgcagagta ggtggagcga
     6661 cttccttcgt ttcaccaaag ttatcttcag ccagctgacg gaaagcgatc agtgcctctt
     6721 cagcttctgg ccctttcgca atcaggcgca gcgtatcgtt atagcgaact tgtagtaacg
     6781 caatctggtt aatactctct ggtgtgacgc atttgccgtt tttttccagc aacatatcgg
     6841 cattaaatgt cgataaggta taaaccagcc gggaggccgg acgtacatgc aggccgttac
     6901 ggttttttat gaccaccgcc agagaacggg cttcttcatc gtacggagga catgtgtcag
     6961 agatttcagt gtcggaggac ggtaaaccca gttgttcact tttggcttcc agcgcatgca
     7021 tggcgtcaaa gataactttg tcgatatccg cccccgaggc cgcgctgacc gttgctgcca
     7081 gtgtaccttc gaccaacggc gcagcacaca aacgtacttt tgcggcgatc tcgggagcca
     7141 gcaattccag cgcagtttca gcactcaata atgcgctacc catatccatc atgaccagca
     7201 catggtcggc atcagcaaca gattcgatgg cctccatcac tttgacggca tcggtaccaa
     7261 tgggattttg tggatcgtca attcccgcgg caatggcgat tttacaacta tcactcatta
     7321 acatctgacg ggctaattca ccgacacctt ctcccagtcg gctgctatgt gaaactatga
     7381 ccaggtttac catcaccaat tccttactct tttgcggcta acgccaacat ttgcatcata
     7441 aacatcaccg aggtcgcgcc gggatcctgg tgaccaatac tgcgttcacc gagataactg
     7501 gcgcggcctt tgcgggcttg catcgtaatc gtactttgtg cagcggattc ggcgatacta
     7561 ctggcagctt cgagcgccac cggaacagag agattttgct cgctggactg acgtaacgat
     7621 tccaccaccg gcacccacac atcacacatg gttttatcgc caggttcggc tttcccgcga
     7681 ctgattacgc cgtccgcgcc atcgcggaac atctgataaa gctcttccag tgtcaggctt
     7741 tgccgtgcct gggtcgcctg tgcggcgcgg ataaagaagg taccgaacag cggaccactg
     7801 gcaccaccga cgctggaaag cagcgtcata ccggtattct tgagaatgaa accgatatct
     7861 ttatctgcga tagcagggag tttttccacc actttgctaa agcctcggtt catatttagc
     7921 ccgtggtcag cgtcgccaat ttcgcgatcc agtccggtaa gatactcgct ctcggtgctg
     7981 aaaatatcgc cacaacgagt gagccagtta acaatttgag ttctgctcag tgacattgct
     8041 ttctccttat ttaccccagt taagggccgg ggtgtggacc ggggcgtccc agagtgccag
     8101 cgtttcgtca tcaactttca gtaaggtgat tgagaaaccg gtcatatcca gtgaggtgca
     8161 gtacgcgcca attaaattac gttcgatagt caatcccgct tgctggcaac gtgtggtcag
     8221 gcggttatag acgccgtaca gctcagaaag cggagttgcg ccaagattgt taaccagcgc
     8281 aatcacccga tcgccagact ggagcggttg tttggtttgt tgttcttcct gccaactgcc
     8341 ttgttgataa tcccagaaac gcaaagtgcg atggtatgag ccatttacca gcagggtgtc
     8401 gaacatttca tcgacggttt gatcaaggga agagaagggg cggcggtcaa tacccggctc
     8461 accatgaatg ccgacgccaa actccatctc attatccgcc agggtaaaag aaggtttgcc
     8521 cgcggcagga acggtacagg caccgagagc gatacctatt gagtggcctt gattattcaa
     8581 cttacgcccc agttccgcac aggcgtccag tgagtcgcca cgctccgccg ctgcgcctac
     8641 gagtttttca attaatacgg tgttggcaac gccgcgtcgc ccggcagtat aaagactgtc
     8701 ttttaccgca acgtcgtcat caatgaccac agtggtcact tttacgccgc tatcgtgcag
     8761 taactcggtc gctgtttcaa agttaagaat atcgccggtg taatttttga taatcatcag
     8821 tacaccttcg ccgccatcaa tttgcatggc gcattcaaag attttatcgg gcgtcggtga
     8881 ggtgaaaatt tcgcccggac aggcccccga aagcatcccc tgaccgatat aaccacagtg
     8941 catcggctcg tgtccgctgc cgccacccga cagcagtgcg acttttcctg caacaggggc
     9001 atcagctcgg gtgacataca ccggatcctg atgcagtgtc agcgatggat gcgctttcgc
     9061 cagtcctgcc agttgttcgt ccagtacgtc ttgcacatca ttgatcaatt ttttcattat
     9121 tttgctccag caattacggt agggcatgga tgatgttcaa cgacacggcg accactgact
     9181 gccgatgaat ccattgtgca tcaggcaagg gaaaagaaaa ttagcccaaa ttatgtttca
     9241 tagtgaaaca tatgctttaa tgaatgttcc atattgaaac ttttacgtgt attaatactt
     9301 aaaattgcga gccggaacac cttttgtcat aagggatgcg ggatatgagt ggcgctttta
     9361 acaacgatgg tcggggcata tcacccttaa ttgcaacctc ctgggagcga tgcaataagc
     9421 tgatgaaacg ggagacatgg aacgtaccac atcaggccca gggcgtgaca tttgcttcta
     9481 tttatcggcg taagaaagcg atgctgacgc tcggtcaggc tgcgctggaa gatgcctggg
     9541 aatatatggc accgcgagag tgtgcgctgc ttatcctcga tgaaaccgcc tgcattctca
     9601 gccgtaatgg cgatccgcaa accttgcagc agctaagtgc actgggattc aatgacggca
     9661 cgtattgcgc cgagggaatt attggtactt gtgcgctatc gttagcggct atctctggtc
     9721 aggccgtgaa aacgatggcc gatcaacatt tcaaacaggc actctggaac tgggcctttt
     9781 gtgcaacgcc gttgtttgac agcaagggcc gattgacggg aacaatagcg ctggcgtgtc
     9841 cggttgagca aactaccgca gctgatttgc cgttgacgtt ggcaatcgcc cgcgaggtcg
     9901 gtaatttact gctgacggac agtttgctcg ctgaaactaa ccgtcattta aatcaactta
     9961 atgccctgtt agaaagtatg gatgatggcg tgattagctg ggacgagcag ggtaatttgc
    10021 aatttattaa tgcccaggcg gcgcgggtct tgcgccttga cgcgacggca agtcagggac
    10081 gggcaatcac tgaactctta acgttacccg ccgtattgca acaagcaata aaacaggcac
    10141 atccgctcaa acacgtagaa gcaacctttg aaagccagca ccagtttatt gatgcggtga
    10201 taacccttaa accgataata gaaacgcagg gaaccagctt tattttgttg ctccatcctg
    10261 tggaacagat gcggcagttg atgaccagtc aattaggaaa agtcagccat acctttgctc
    10321 atatgccaca ggacgatccg caaacccgcc gcttgattca ttttggtcgc caggcggcgc
    10381 gcagtagctt tcctgtcctg ctttgtggag aagagggcgt gggcaaggca ctgctaagtc
    10441 aggcaattca taatgaaagc gagcgtgctg caggtcctta tatcgccgtc aattgtgagt
    10501 tatatggtga tgctgcgctg gcggaagaat ttattggtgg cgatcgcacg gacaatgaaa
    10561 atggccgtct gagtcggctg gaactggcac acggcggcac gctgtttctt gaaaagattg
    10621 aatatctggc ggtggagtta cagtctgctt tgcttcaggt tatcaagcag ggggttatca
    10681 cgcgactgga tgcgcggcgt ttaataccaa ttgatgtcaa agtgattgca acaacgaccg
    10741 cggacctcgc aatgctggtg gaacaaaatc gttttagtcg ccagctgtat tacgcgctgc
    10801 atgcatttga aattaccatc ccgcctctgc gtatgcggcg tggcagcatt ccggcgctgg
    10861 tgaataacaa attacgcagt cttgaaaaac gcttctctac gcggctgaaa attgatgacg
    10921 atgccctcgc tcgcctggtt tcttgtgcat ggccaggcaa cgattttgaa ctttacagcg
    10981 tcatcgagaa tcttgctctg agtagtgata acgggcgcat tcgcgtcagt gatttaccgg
    11041 aacatctgtt taccgagcag gcaacagatg atgtcagcgc cacacgcctt tccaccagtc
    11101 tgtcatttgc ggaagttgaa aaagaggcaa ttattaacgc agcccaggtc acaggcggtc
    11161 gcattcagga aatgtcggct ttacttggaa tcggccgcac tacgctgtgg cggaaaatga
    11221 agcaacatgg cattgatgca gggcagttta agcgccgggt atgaaagaca gaaacgattt
    11281 ctgatacatc agagtgatct atatttcatt ccggcgcacg ctaacatttt tcagcatcgt
    11341 ttaaggactt gtctatcccg cacttaaaag ctgaagcgat atcctccgtt gacttgcttc
    11401 tgatcaaaga ggttaccctg cgtgtaatcc gcttcgagat agaatgtgtg ctgtttgtta
    11461 tactgtgcac tgactcccac gccgttattc cagccattac ctttgaaact gtacttctcc
    11521 cgggagttgt tcaacagata ttcggtatcc cctgaaaact cgcggatagc gccagtcttc
    11581 acatagacat tcagctggct gttgcctgcg gtgatgtcat accccagtat catgctggca
    11641 cgccccagca gcgattcgta gtgattcaga tgtatattga ggccattact cgccttcata
    11701 gccatctcat tctggtggct gtatgtaagc tgggtttgcg gctctatata gaacccataa
    11761 ccagtagggg acaggttgaa cctctgcccg gcttccaggg agatgctcat tccattcgca
    11821 gtgccgttgg cgttaacgcc gttgttctga ctgtccagta cgtggaaact atttttctgg
    11881 cgcgatgctt ttataacgag atcgctgtaa aaaccgtttt gtgccatgta actggcgtac
    11941 attcccatgt agtctgaacg tgcggtaccg tcgcctccgc tatagtccgg cgatgcatgt
    12001 gttgagccaa tatacagacc gacatacaac ggcattacat cagagagacg tttatcccca
    12061 ccaaactgga taccgctgta acccatgtca aagccgctca gtttgccact ggcaaaggag
    12121 tccaggcttc ccccataact gcgcaaccag atattaccgt ctttactctg atttcgcagg
    12181 tcacccatcc gttgcatcag cgtacggttt tcaacatagt tcaataagta accgacattg
    12241 agataattgc cgccagcatc tgcggtcgtg gtgggtttag gcgtgggagc gggttcaggc
    12301 gtaggatcgg ggttgactat gggaggctga gcgggagctg gtgtgggttc aggattagga
    12361 gtcggttccg gaactgtccc ggaagcgtaa agctcccagt tagtgccatt tttacgcaca
    12421 tcgtacagat atccccccaa ctcaacctga gaagacgcgc tgaacgaggc cgcgccgtca
    12481 gtggttttta ccactgtcag aacttcattt cccgttgtgg cctcgctgcc ctggttgcgg
    12541 atagccaata cgtgattacc agcactactc ccgctgatat tcaataaatc ccctttatta
    12601 ttaacgccat taccctcgcc aacaacatca gcacgcataa taaaggtact gttaccgctc
    12661 aggttctcta cgtttaatgt ggcaaatgtg ccggcagttg acccgtggct ggcaaaatcg
    12721 acagttgaat ggctcagcgc cagcgtgtcg agattagaat tacttgttac gttccagacg
    12781 ctgttattca ttgcaacgtc cagttttccg ccattgacat tatcgctgag ggaggaacct
    12841 gtccaaaccg acccagggtg catatccaga ttgattagcc cacctttgga aagaacgcta
    12901 ccgttgataa gcatacgacc cgaggcgttg atgcggctgg cggcataacc atcgtcatgt
    12961 tgcgttgcaa tggccatctg gtcgggtgta ctcatatcaa tgaccagatc gctcgtgagg
    13021 tcgatctggc tgttggtcat ggcataaatt cccctggctc ctgccgcgcc ggtgatagcc
    13081 aaactgtctc cggttattct accgccgctg agcgcccaca aacccagcgc cagactgcca
    13141 ttacgatcaa ctgtaatatc ggtattttgc atgttgacaa cagccgttgc cgtctgggcc
    13201 gaggcaccat aagaaccgcc ggaaaagatg ctgtttcgtt gcgctgccgt gccagaaaaa
    13261 ttgattgtcg cgtctgaacc actggtgacg aggccaccgc cctgcgcgga agaaatatgg
    13321 ctatctgcac cgatagtggt tgtaccacca cgaacttcga cgccattggc cgcggctcca
    13381 gttacatcaa cagtgagtgc attcgcgcta acctggccaa agctccagag gccgtgtgca
    13441 ttatcgccat tggttttaat ggaactgttt gttccgagat cgacaacaga gtttttctgt
    13501 acgtttatcc ccatggcgct gtagccctga acatcgattg tcaggtctgt agccgtaaaa
    13561 cgcgcagcac cattggcgtt attgccgttg agaccaccga tataaacacc tgtacttcca
    13621 tcggtcgtga ttttacttcc gcttccaaga tcgacactgg tgccatagtc attgatggtt
    13681 aggcctatac cgttcgagtt ttcaatggtg aattgagtgg ctgttagtgt tgagctatgc
    13741 ccaataatga tgccgtcatc gttactctta atggtgctgc ctgtgcctaa gtcagcatgg
    13801 gtatagtcac caattaagtt aatgccgatg gcagaggttt gaccaacaac atctactgtg
    13861 agtcggttag ctgtcaggga gctgtttttg ccctgaataa tgattccttt tgcatatcca
    13921 gaggcgtcgt tgacattgac ggtgacatca ttacccaggt tgatattgcc aggcgtgttt
    13981 cctgctgggg tcattacacc atagaggttt cctgatggat cggcggtatc tcctgtaatt
    14041 ctgtcaccat cattaatagt aatattggtg ccatattgac ttcctgtatc agcagctatc
    14101 agcagggggg aaaagataat tgcattcaaa ccaatagcgc caaattttat cagttgaatt
    14161 gagggcgagc ggatttttaa ttcagcgaga cgatctgctt tggtattccc attgtgttgt
    14221 ttgatgccca taccactcct atatagtacc cagactatgg gtctatggat tttgtcattt
    14281 tgtttaaatc aatgaataat atcctcttat catcatgata tttgtatggt gcaatcatga
    14341 tgagcttctc agagaataat tctctattac cgggcaaaat caggaacaca ggaattaata
    14401 tatatttaac atccccctta caaaggtgag tttatatcga gttattccct aagttgtcgt
    14461 tacttattcc aattcgttac tataaatatg gcaaaaatat tacaacaaac agcggttaaa
    14521 aaatttggtg aagatttttt gtaataatcc taattagtta tgggaatttc gcttgatgta
    14581 tctgcataac aaaaacaata tgcgccattt ttgcattact aataagaaaa agcaaaaaac
    14641 atcgatattc atttttggtc aatgggtttt ctggcctatc gttgttattg tgctactggt
    14701 tatcattttc atcttgccag catattggag cgtgatcaat tttgatcagc tgtgaaggtg
    14761 gctgatgtgt agtgatacaa aaacttacgt tatatcactg ctaatgagat atccgtcatc
    14821 tgaacactta tgtctttaat tattctcgtg gttcactata ggcaataagc acaaaagtgt
    14881 aggatgttac aagaatgatt aggactcggt gaaatgaaaa atccacgcaa ttgcgtggat
    14941 tttatatact tttgcgctct tcatgagatt tagcgaaacc tcatgagaca ataaattaat
    15001 tagacgttga aaaggaagtt catcacatcg ccatctttca cgatgtaatc tttaccttct
    15061 gcacgcattt tgcctgcttc tttcgcgcct tgttcacctt tgtaagtgat gaaatcttca
    15121 aacgagatgg tttgtgcacg gataaagcct ttttcaaaat cagtatggat tttgcccgct
    15181 gcctgcggcg cggttgctcc aaccggaatg gtccatgcac gcacttcttt caccccagcg
    15241 gtgaagtaag tttgcaggtt cagcagctta taaccggcac ggatcacacg gttcaggccc
    15301 ggctcttcca gcccaagctc ctgcataaac tcgtcacgtt cttcgtcgtc cagttcggca
    15361 atgtctgctt caacagcagc acaaaccgga accacaacag aaccttcttt cgccgcgatt
    15421 tcacgcacct ggtcaagata tgggttgttt tcaaaaccgt cttcgttgac gttggcgatg
    15481 tacattgttg gtttcagcgt caggaagctc aggtaacgaa tagccgcttt ctcttcagcg
    15541 cttaaatcca gcgcgcgcag catacctgca ttttccaact ggggcaggca tttttccagg
    15601 accgccagct caactttcgc gtctttatcg ccacctttgg ctttcttctg tacgcgatga
    15661 atcgcacgtt cgcaggtgtc gaggtctgcc agcgccagtt cggtgttgat aacttcaata
    15721 tcgtcagccg ggttaacttt gccggaaacg tgaatgatgt tgtcattttc aaagcagcga
    15781 acaacgtgac cgatcgcttc ggtttcacgg atgttggtca ggaactggtt acccagacct
    15841 tcgcctttcg atgcgccttt taccagaccg gcgatatcga caaattccat ggtcgtggga
    15901 agcgtacgtt gcggttttac gatttcagcc agttgatcca ggcgagggtc aggcattggt
    15961 acgacgcctg tgttcggctc aatggtgcag aatggaaagt tggccgcttc aataccggct
    16021 ttggtcagcg cgttgaacag ggtagatttc ccgacgttgg gcaaaccgac gataccgcat
    16081 ttgaatccca tgatttaaat caccttaata tcttaataat caacctgtta ttgctaacag
    16141 attgcagaaa tggaaataac tttgcctatt atacacggca ctcggcaaaa atgccgcaga
    16201 caacgactta ttgcgcttta aaggcgtgca atcggttcgt tgctttggtc aagccatctg
    16261 taaaccacat ttcagtacaa cgcgccgctt cgtcgatggc ttcatcaatt aacttctgtt
    16321 cactaacagg cggtttgcct aacacaaaac cgacaacttt atttttatcg cccggatgac
    16381 cgattccgat gcgtaaacgg tgaaagttag ggttattacc caatttactg atgatgtctt
    16441 tcagtccatt gtgaccacca tggccaccgc ccaatttaaa tttggcgacg ccaggaggca
    16501 gatccagttc gtcgtgggcc accagaattt cgtccggatt aatgcggaaa aaactggcca
    16561 tcgccgcaac ggctttgccg ctgagattca taaatgtagt cgggactaac aggcggacat
    16621 cttcgcctcc aagagtgact cgcgaagtat aaccaaagaa tttagcctct tcgcgcagcg
    16681 gagcgcgcaa acgctctgcc agtaagtcaa cgaaccaggc accagcatta tgtcgcgttg
    16741 cggcgtattc agcaccgggg ttcgccaggc cgacaatcaa tttaatcgtc acgttttttt
    16801 gtcctgagtg tgtacataac tggcgcgtag tttactggtt gcggccccgc ttgacaaaaa
    16861 actgcgaatc aaatgcagat aacgtaataa ttgcatgagt ggactattag aaagtcaagg
    16921 tgttcaggcg tttatttgta aagttttgtt gaaataaggg ttgtaattgt gatcacgccc
    16981 gcacataacc cacagggtgt tgtctatact ttacacataa ggaagagggg tattccctgt
    17041 tacaacccag aaagttccgg aggtgacata tgaaacgcaa aaacgcttcg ttactcggta
    17101 acgtgctcat ggggttgggt ctggtggtaa tggtggtcgg cgtggggtat tcaatcctca
    17161 accagttacc acagtttaat atgccccagt atttcgcaca tggtgcagtg ctaagtattt
    17221 tcgtcggtgc cattctctgg ctggcgggtg cccgtgttgg cgggcatgaa caggtgtgcg
    17281 accgttactg gtgggttcgc cactatgaca aacgttgccg ccgtagcgat aatcgccgtc
    17341 atagctaaca aataatgcag tttgctgacc agtcagtttg cgctgactgg tcaatctcgt
    17401 acttataaat ccgccatcgc cgcgcgacga ttcgggaaga acgccaggcg tcccgggatc
    17461 ggttgaatgc cagcgcgagc catagtgcgc agtggctgga attccacgtt gcacacgcgc
    17521 agttcacatc cctcgggcag acgcttcaca aaacgctgga acgcatcaag accaccagca
    17581 tcaagtaccg gaaccgcatc ccacttcaga atcacaatcc gtttgccttc aagacgtgac
    17641 tccaggtccg tgaacaagcc ttcagcagca gcaaaaaaca gcgggccaat aacgcgcaga
    17701 accaggacat cgtctggaac atctacgact accggtgcca ggcgagtcat acgtgcgata
    17761 cgacgcataa acagcagcga tgccagcacg atccccacgc tgatggcaat aaccatatca
    17821 aacagcacgg tcagcgacat gcacagcagc atgacaatga tgtcatcttt cggcgcatga
    17881 cgcagcaagt cgaccacttt gtgcgcttca ctcatgttcc acgccaccat caacagcagg
    17941 gcagccatgg cggaaagcgg cagccaggag agcagcggtg ccagtaccag cagggcaaga
    18001 ataaccagaa tagagtggat caccgccgag ataggggaag ttgccccggc acggacgtta
    18061 gcggcagaac gcgcgatggc agctgtagcg gtaatgccac caaagaacgg agcgatgata
    18121 ttccccagtc cctgtccaac cagttcgcta ttcgccttgt gtttcgtccc ggtcatacca
    18181 tccagcacca cggcgcagag cagagattcg attgcgccga gcattgccat tgagaatgcc
    18241 gcaggcagca gtgtgcgaat agaatcccag gttagcgtga attctgaatt aggcagatcc
    18301 cacggcagca ccagttgcgg cagcagttgc ggaataccgt taccctgaga accatcggcc
    18361 aggacgtagt ggaattgcga accgatggta gcaacatgtc cgccgagcag gttaacaatc
    18421 cccatcaccg cgcaaccagc cagcaaggcc ggaaggtgac cgggtaaacg aatgcccaga
    18481 cgcggccaaa aaacaagaat acctagcgtc acaatgccaa tggcagcatc acccacatta
    18541 atggtcggca gcgccataaa taatgcgccg actttttgta gataatgttc cgggacatgg
    18601 gccatttgca gaccgagaaa atctttaatc tgcatggtac cgatggtgat cccgataccc
    18661 gaggtgaaac ctaaggtgac ggaaaccgga atatactcaa tcaggcgacc aaagcgtgcc
    18721 agacccatca gaatcaaaaa gatccccgac agcaaggtcg caaccagcag tcctgccagt
    18781 ccaaactgtt gcgaaacggg atagagaatt accacaaatg ccgcagtcgg accggaaacg
    18841 ctaaagcgtg acccacccgt cagagcaatg acaatccccg caacagctgc ggtatataaa
    18901 ccgtactggg gtgccacacc actaccaata gccaacgcca tcgccagcgg gatagcaata
    18961 atcccgacgg ttatcccggc aatcaggtca cgggtaaacc gtgcggcagt atatttttct
    19021 ttccagcaag cgtcgatcag agcgcggaaa ggcatcacat gtgaggaaaa tattttgttc
    19081 acaataatgt ttcatccgtg agcgcatcat ctgtcaacta aatggcaggt gaaggaggca
    19141 taggtcatac aaatggatat tacagacaaa aaaacccgcc gcagcgggtc tttgagccgg
    19201 gttcgattag tgttcgaaca tggcagagat cgattcttcg ttgctgatac gacgaatcgc
    19261 ttcggccagc atacctgaca gggtcagagt acgcacgttc ggcagtgatt tgatttcatc
    19321 gctcagcgga atggtatcgc agacaacgac ttcatcaatt acagagttac gcaggttgtt
    19381 cgccgcgttg ccagagaaga tcgggtgagt cgcgtacgca aatacacgtt tagcaccacg
    19441 ttctttcaga gcttcagcag ctttacacag cgtaccgcca gtgtcgatca tatcatcgac
    19501 cagtacgcag tcacgacctg caacgtcacc gatgatatgc atcacctggg aaacgttcgc
    19561 acgcggacga cgtttgtcga tgattgccat atcggtatcg ttcagcagct tagcgatagc
    19621 gcgggcacgc acaacgccgc cgatgtccgg agaaaccaca attgggttat ccagattcag
    19681 ctgcagcatg tcttccagca ggatcgggct accaaatacg ttatcaaccg gaacgtcgaa
    19741 gaaaccctga atctgttcag cgtgcagatc cactgtcagc acacggtcaa caccgacgct
    19801 ggagaggaag tctgcaacca ctttcgcagt gattggtaca cgagcggaac ggacgcgacg
    19861 gtcctggcgc gcatagccaa agtaggggat aacagcggtg atacgacctg cggaagcacg
    19921 acgcagggca tcaaccataa cgactaattc catcaggttg tcgttagtag gggcacaagt
    19981 ggactggatg atgaaaatat caccaccgcg tacattttca ttaatttgta cgctgacttc
    20041 gccatcgcta aagcgaccta cagcggcgtc gccgagtgaa gtgtacaggc ggttggcaat
    20101 acgttgtgct agttccgggg tggcgttacc agcaaaaagc ttcatatcag gcacgagaag
    20161 aacctcaggc atgcgtccat tggtggaaag aatctgccga aaactgtgcg ggccaggcat
    20221 gatcctttcc aggcggtgta ttaaagagcg cgatgcaacg tctggaacaa ggtgacgttg
    20281 tcaccgaaac tcagcttgcc cggcttaaag catggctctg tgcaatgggg aaagattaac
    20341 gcctttcgcc acaaagccat tgagccattc cggggcttgc tctagcacct ggcgggcttc
    20401 agactctgta tcaaattcag caaagacaca ggcccctgtc ccagtcaggc gcgacggggc
    20461 gtattctaac agccaggaaa gcaccgcatc aacctcgcga aaacgttttc ttgcgataac
    20521 ctcgcaatca ttgctgaatt cacattttag caacgtttct attgaccttt ttggcgtatt
    20581 gcgcgggagt tcaggatctt taaaaatcac cggagtcgga atacttacac cagggtgcgc
    20641 caccagatac cacttctctg gcggatccac cggcgttagt atttcaccaa cgccttcggc
    20701 aaacgcggca tgcccccgaa caaagacagg aacatctgcg cccagcgtca gccccatttc
    20761 cgccagctca tccatgctta gcccgcattg ccagagatga tttaatgcca ccaggaccgt
    20821 cgcggcattg gatgaaccac cgccgagacc gccgcccatc ggcaaacgct tgtcaatgct
    20881 gatattcgca ccgcttcccg tcggaagacg cccgctgtct gccgcagttt ttatcaacaa
    20941 tcgcgctgcg cgaacgatca ggttatcttc atgttccacg ccttcaacgg gcgttaacag
    21001 acgaatatcc ccatcgtcac gaagctcaat gctgatggtg tcgccgtaat caagaaactg
    21061 aaacagcgtt tgcagcgtgt ggtaaccatc cgcacgctga ccggtaatgt ataaaaacag
    21121 attaagtttt gccggagagg gccactgtgt ccgcattatt tcactatcca gttatccatt
    21181 tttaacttga tgcgttgacc accgtcggtg agttccatat tggctggcat cgcaggttgc
    21241 gttttggtgt cataaccacc ataaacaacc ttccagtttt tgccattctg gctgtaggta
    21301 atttcgctca ggcggtactg gtcgtccagt ttgtagtcgg ttgcatcacc cggtaaacct
    21361 aaaatccact ggcgcaagct gttgagcgga attggcattc cggtcaattt gccaatcatc
    21421 tcttcggcgt catcggcggt ataacgctga cctttattgt cgactaactg cacgttaccc
    21481 ggttgagcat tcagctccag ttccgtgctg cccaatgggt tagtgagcag cagacggtag
    21541 cgatcctggc cggtttgctg ccagaaaaag cgggcgtaca ctttttgttg gtcagaaata
    21601 taagcgaacg cgccgcgagt ctgatactga ttaagattgc gcacgtcttg ctgatgctga
    21661 cgccattgtg g
//
LOCUS       DAEVZA010000086        21454 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEVZA010000086 DAEVZA010000000
VERSION     DAEVZA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.086"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..61)
                     /locus_tag="LAF92_003194"
     CDS             complement(<1..61)
                     /locus_tag="LAF92_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792156.1"
                     /translation="MASVSISCPSCSATDGVVRN"
     gene            complement(119..295)
                     /locus_tag="LAF92_003195"
                     /pseudo
     CDS             complement(119..295)
                     /locus_tag="LAF92_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            478..699
                     /locus_tag="LAF92_003196"
     CDS             478..699
                     /locus_tag="LAF92_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBI9792157.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            699..1079
                     /locus_tag="LAF92_003197"
     CDS             699..1079
                     /locus_tag="LAF92_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBI9792158.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGGISVSSVADTLRRLYGSSE"
     gene            1084..1263
                     /locus_tag="LAF92_003198"
     CDS             1084..1263
                     /locus_tag="LAF92_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HBI9792159.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLVGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            1291..2334
                     /locus_tag="LAF92_003199"
     CDS             1291..2334
                     /locus_tag="LAF92_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBI9792160.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDSLVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSDSH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKAAPQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGAITSYSSSVR"
     gene            2425..2790
                     /locus_tag="LAF92_003200"
                     /pseudo
     CDS             2425..2790
                     /locus_tag="LAF92_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
     gene            3011..3130
                     /locus_tag="LAF92_003201"
     CDS             3011..3130
                     /locus_tag="LAF92_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBI9792161.1"
                     /translation="MVVLAGLPKGRPVPFDAGSLNPVSATAIEIETSRGSSLT"
     gene            3149..3370
                     /locus_tag="LAF92_003202"
     CDS             3149..3370
                     /locus_tag="LAF92_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBI9792162.1"
                     /translation="MATVPTPIHPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDLRNSLSYWCLPVNASAQEGL"
     gene            3367..4482
                     /locus_tag="LAF92_003203"
     CDS             3367..4482
                     /locus_tag="LAF92_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBI9792163.1"
                     /translation="MRTSLVTREEMIEAIEQHTACISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAEARKEAHRMKATNVKSEKVHAPSVQES
                     ELVVVQNQSDEIPVLEWQGVRVVTTETLAKGYGTEAIRIRQNHHENKVRFVEAKHFFK
                     VIGDELKNLRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWAFFEKLEDSY
                     FRQKEQQPVAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAASKVDFADRVSV
                     AKGILIGNFAKVVGLKQNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLD
                     DEDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(4515..5366)
                     /locus_tag="LAF92_003204"
     CDS             complement(4515..5366)
                     /locus_tag="LAF92_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792164.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDTASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DNPESAQ"
     gene            complement(5477..5686)
                     /locus_tag="LAF92_003205"
     CDS             complement(5477..5686)
                     /locus_tag="LAF92_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HBI9792165.1"
                     /translation="MAFIQPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQLDSQ"
     gene            5874..6854
                     /locus_tag="LAF92_003206"
     CDS             5874..6854
                     /locus_tag="LAF92_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HBI9792166.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWYFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
     gene            7491..7712
                     /locus_tag="LAF92_003207"
     CDS             7491..7712
                     /locus_tag="LAF92_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HBI9792167.1"
                     /translation="MGYSAAKVSTHLELEKNRGYWRAKGFDRDSCQLSLSRGEEKIVR
                     TRGRWRFYGENHKQVKAEPILYTLLKTII"
     gene            7720..8751
                     /locus_tag="LAF92_003208"
     CDS             7720..8751
                     /locus_tag="LAF92_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI9792168.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDIRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLQRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEISRIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEDGD"
     gene            8802..9113
                     /locus_tag="LAF92_003209"
     CDS             8802..9113
                     /locus_tag="LAF92_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HBI9792169.1"
                     /translation="MRKDFNIDGKYVVLSVSTNIQSPAVIVTVKLSDRMPDIDSISVA
                     FPVKSMRGAEHFVMNATEEEARRGFAKVVSDFGELLDKVNNVLSISSARSKALTASMM
                     K"
     gene            complement(9306..9413)
                     /locus_tag="LAF92_003210"
     CDS             complement(9306..9413)
                     /locus_tag="LAF92_003210"
                     /inference="COORDINATES: protein motif:HMM:NF013676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBI9792170.1"
                     /translation="MAESCPGTSRASLENMQAWYIRDETYSAALAELVK"
     gene            9462..10102
                     /locus_tag="LAF92_003211"
                     /pseudo
     CDS             9462..10102
                     /locus_tag="LAF92_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(10209..10610)
                     /locus_tag="LAF92_003212"
     CDS             complement(10209..10610)
                     /locus_tag="LAF92_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001541954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBI9792171.1"
                     /translation="MSVEENIKTVKKFYRLIEENKYDEVKNLCHPDFKFYSQVDTPLN
                     SEQFIVQEKGHMDAFPGFTMRIHEIFARDDKVACYLIFEGVQTREFLGHPASGRKVRF
                     SLMFMITLKDGKYIEKRAHYNTADILRQLSA"
     gene            complement(10619..11608)
                     /locus_tag="LAF92_003213"
     CDS             complement(10619..11608)
                     /locus_tag="LAF92_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI9792172.1"
                     /translation="MSTITVAIVITEGFSTFHVSVPLILFGEILEGKALFSRRVCAEE
                     PGLIWSAEGTAMRAEYGLDALAEADIVIIPFWKHIHERPSETLLSALVAAWKNGAQVV
                     GLCLGSFVLAYTGLLDGKRAATHWEAERDFQMLFPAVHLDVNVLYVEDERIITSAGTA
                     AALDCCLHVIRQRLGRYTANQIARRMVAPPYREGGQAQYRQLLIPKSTSDNRINQLLE
                     YLSANLGLPHDIDTLAHLASMSRRTLTRHFHSATGMTVWEWITTERLRRSQELLESTD
                     IAVERVAELAGFQSTITFRQIFRQRMGVSPSEWRKTFNGTPEGNIPAGKETEK"
     gene            complement(11624..12400)
                     /locus_tag="LAF92_003214"
     CDS             complement(11624..12400)
                     /locus_tag="LAF92_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBI9792173.1"
                     /translation="MKIIQVRNATQLISYAGKKFLVDPMLAKKEAYPGFEGTARSDIR
                     IPMTELPFDLDVLLDVDAIIVTHTHPDHWDEAAVAHIPKDKLIYVQNENDEKILRSQG
                     FSNLVILSDKSTFGEISLIKTVCQHGSDEAYANPQLAAILGDACGVVFQHPSEKTLYL
                     VGDTIWIKAVEDNLRRFNPGVVIMNTGWAHVLGFGPIIFGREDVLKAHRIVPEALIVA
                     THMEAVNHCLLTRQELLEYARDNQIQASVAAPADGESVTI"
     gene            12619..13005
                     /locus_tag="LAF92_003215"
     CDS             12619..13005
                     /locus_tag="LAF92_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /protein_id="HBI9792174.1"
                     /translation="MSASTKNHLNVQYLPFPKRSPPTTEERRIANALGALPCIACYMP
                     GVISNEVSLHHIAGRTAPGCHKKQLPLCRWHHQHAAPAEVREKFPWLVPVHADGVVGG
                     KKEFTLLNKSEMELLADAYEMANIMH"
     gene            complement(13052..13606)
                     /locus_tag="LAF92_003216"
     CDS             complement(13052..13606)
                     /locus_tag="LAF92_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792175.1"
                     /translation="MKNRSFNIIASIAMALMLTGCNQSEIDKLSLIGEDKSYLEEQDE
                     ATFEKRTPDIELFALYDKSSPDDQEPHTTGMLINGKVELSMTTKVGKYTFMQLDEMLT
                     KEYGKPVATKDHIFDKAAISGLDCIETKTCGAGKYYEVFRGKDRFVLLINGAGYLYEK
                     EGVALLTITDKQLKVARLEEDKKH"
     gene            13887..14528
                     /locus_tag="LAF92_003217"
     CDS             13887..14528
                     /locus_tag="LAF92_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HBI9792176.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCSFATEYNN
                     VASADYIRAMAGEVESNKEPSLLSPLINIIRGTQCLADKRVPQGLLTGKENYL"
     gene            14622..15659
                     /locus_tag="LAF92_003218"
     CDS             14622..15659
                     /locus_tag="LAF92_003218"
                     /inference="COORDINATES: protein motif:HMM:NF025539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792177.1"
                     /translation="MNIKPLKISNKVMKPHKASLNPDLTARNVLTYSHWDFVELWLKR
                     NEKDEALFYWEQAKVFNQAANGLPNQSAPLLHYYSFMNAVKALLSSRNINFKQHHGVA
                     RGDNAAQINNLSDIKVKIKNEGILPSFSKYIDGASHEGVYNIKSLFSGLPYIHRTYCL
                     TYDVTDDIFIPLIDAEFVLNESDNSLFFTAILSKDFRFDTVFDILPPAFEMFEIEKYK
                     IRSVESLHDFNENQPFAMSQLTEFHKKIRRNLYYINGAETLWYFKSKNKQSEHTPVSI
                     SPLVITLAAMHRLSELCRYDPLKLRQLLNEKENWIIAEFIQQSPSQFIDAISSEITGH
                     QFLIPNVRSAS"
     gene            15659..16168
                     /locus_tag="LAF92_003219"
     CDS             15659..16168
                     /locus_tag="LAF92_003219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792178.1"
                     /translation="MNIYIACALTHVPREIFHEYSNWIHSLAKGLSQNNNVKYALINS
                     DPALSKRPESNKSRLCYIWDRDMVEKSDVIIAECSFPSTGLGIELQIAEQNNIPVIIC
                     YKDYGINKTKTIEYVNPDETTHNLQVGEGFISHMVLGLPNILDVILCKDIDNTCRKIK
                     ILLDMINHN"
     gene            complement(16227..16475)
                     /locus_tag="LAF92_003220"
     CDS             complement(16227..16475)
                     /locus_tag="LAF92_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modulator protein"
                     /protein_id="HBI9792179.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGK
                     WRTVVDFKTNSVPQGVRDRAFEEWEQIIIDNALLLNAD"
     gene            complement(16472..16912)
                     /locus_tag="LAF92_003221"
     CDS             complement(16472..16912)
                     /locus_tag="LAF92_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBI9792180.1"
                     /translation="MATLSDTIKPNKTYLEAVLRTALLGKTEDEYVDFFLSGLRGRLL
                     KNPRLYRSYGPYWPEIKKLLLERGYGNFGRLVDRDVRKIYRYDRPALTLIAATLYSQE
                     RFDNGQIYSAWHLLPVSEEVDDQDYEFESYDLEVEALAQAGEKT"
     gene            complement(16946..18457)
                     /locus_tag="LAF92_003222"
                     /pseudo
     CDS             complement(16946..18457)
                     /locus_tag="LAF92_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            complement(18455..18619)
                     /locus_tag="LAF92_003223"
                     /pseudo
     CDS             complement(18455..18619)
                     /locus_tag="LAF92_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
     gene            18788..19276
                     /locus_tag="LAF92_003224"
     CDS             18788..19276
                     /locus_tag="LAF92_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI9792181.1"
                     /translation="MAQRGVNKVILIGTLGQDPDIRYIPNGGAVGRLSIATNESWRDK
                     QTGQQKEQTEWHRVVLFGKLAEIASEYLRKGSRVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSSNGWGQSNQPQNHQQYSGGGKPQSSANNEPPMDFDDDI
                     PF"
     gene            19525..20880
                     /locus_tag="LAF92_003225"
     CDS             19525..20880
                     /locus_tag="LAF92_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HBI9792182.1"
                     /translation="MTHCKENTWYERQGYNTTRPNEIRSEYERDLSRLIHSSAFRRLQ
                     SKTQVLGLGESDFYRTRLTHSMEVAQIGGGILSQLSKRDTDEGGKFLPDPSLMQAICL
                     AHDIGHPPFGHGGEVALNYCMRSYGGFEGNGQTLRILSKLDKYTETNGLDPTRRLILG
                     VLKYPASYSEVVNERAYSQLQPRSNEWLFKSSEFKPPKCYLDSEENTVRFALQPFDSD
                     DVKLFKTVKTTALNKHKKTIYKALDTTIMDLADEISYSLHDLEDAISLKMIDKKMWEE
                     HFEDKSHLFAACQCDSFNLKADDVAEKLFGESYLRKECIGTLVHLMITSVKLEVQNSD
                     FKSKILRYKVALPAAVEELRKAIFELVRNKVIQHENVQLLEFKGQKLIVELFNVLAND
                     PIRFLPTETRKKYNQEEAKGDDKKMRVICDYVSGMTDDYATRLYEKIFVPRKGSIFDR
                     L"
     gene            complement(21087..21446)
                     /locus_tag="LAF92_003226"
     CDS             complement(21087..21446)
                     /locus_tag="LAF92_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013096817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792183.1"
                     /translation="MSEVFVNNWLPGICTFFLGIFYSNIVEKKKLKQKLKNDILEIFI
                     PVFNAGNEISIEIAENAYRNMNGTFQLYKRIYPGMFNKEAERELDRLLKDGFLINGEV
                     NKHYFEPTNIESLIKRL"
BASE COUNT         5898 a         4921 c         5024 g         5611 t
ORIGIN      
        1 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca
       61 ctggagaacc tcaaagacac catcatacac taaatcagta agttggcagc atcacctaac
      121 agatgctgtc atcaccggct tcaatatagc tggaaaaatt ttcgctgata aaacggtaga
      181 aaagcgcgcc cagaacgtat tgcttaaaat cccatccatc gaccgaacct ctgacatcgt
      241 tagcaatttg ccagatttga cgatgaagct ctgcacgttg ttgaatactt gtcattttca
      301 tccacttatt tcaggcttat gtaattggcg gtgattctac agcaacttgg atgctttagc
      361 agttcggaca ttaggctacg aatgacctgc ctagaggttt gttaagccgc aaagtgctgg
      421 tgctttatgt ctgtgaagtt tataattgtg tacacataac gagtacacga ggtatttatg
      481 caatccatta acttccgtac cgcgcggggc aacctttctg aagtgctcaa caatgttgag
      541 gccggggaag aggttgaaat cacccgcaga ggccgtgagc cagcagtaat tgtcagcaag
      601 gctactttcg aagcctacaa aaaagcggcg ctggatgctg aatttgcatc cctgtttgac
      661 accctggact ccaccaacaa ggaactggtt aaccgataat gaggcatata tcaccggaag
      721 aacttattgc gcttcatgat gcgaatataa accgctacgg cggcctgccg ggcatgtcag
      781 atccgggtag ggcagaggcc attatcggga gagttcaggc cagagttgcc tacgaagaga
      841 tcaccgacct tttcgaagtc tccgccacct acttggtggc tacagcgaga gggcatatat
      901 tcaatgatgc caataagcgt accgcgctaa acagtgcgct gttatttcta cgccgtaacg
      961 gggtgcaggt atttgattca cctgaactgg cagaccttac tgtaggtgct gcgactggcg
     1021 ggatatctgt atcttctgtc gccgacacgt tacgtagatt gtatggttct tcggagtaga
     1081 ttaatggcac gcaaatacaa caaattgtcc cgtgaagcgt taaagatgct tcttgatggc
     1141 gtgagtcgcc gcgaggtaaa gcaatacctg gttggtaagc aaattggagc caggactgct
     1201 attgctgtgt tatgccgtca ggaaatggtt gtgcttaaac agagaatgcc gggcagcaga
     1261 taaagcccaa tcagtgatga aaggtgtgat gtgaaagccg taattactcc ctttgtacaa
     1321 aaagagcttg gcgtcgccac attcaaagtg gatcaggaag tcagaaagct ggtggaggct
     1381 ggccgtaaat ttattatgga gccggtgccg cgtgagttaa tcgagcacat ggacgacggc
     1441 ctcgttgttt ccgagcaaac tatggcaaca aatgaggcgt tgcagccgtt ttttaacagc
     1501 gatgaactgt ttcgccgtat tggtggaatt gactcgctgg tggcgtggtt gcgcaggaaa
     1561 gaggggcaat gccaggccgc agatcgtagt tggtgtgaca accatattgt ccacgctgaa
     1621 cgagacaata gcgcggtgtt gttgtgctgg catcacgata accattaccg gatgcgtggt
     1681 tttaatgagc tgaaagaaac gctgcacaat aatcgcgtta actggatact ggatgtcgcc
     1741 cgtcaggaaa tgggcctttc agatagccat gatttaagta ttcaggagct gtgctggtgg
     1801 gctttcatgc gtaacatgat gcacctgatg ccggaagaag tctgccgcat atcaataaat
     1861 aagatgaagg ctgctccgca ggatagcgga cctctgaaag aggcggatat tcgcccgtat
     1921 gacgatcgcg ctacagcata tgttcagatg atggaagaac gcgccgcgcc gatgcgtgca
     1981 aaagtatgcc ctgtggatgt tgactccgac ccaggtatgg cgcatttcaa aataccaaaa
     2041 cttcaatcgc taaaattgcc cgagtacatg gactttgtgg cttcccgtcc atgctgtggc
     2101 tgtggagctg cgggagctgg cgctcacatt acgccttata tcgttcgtca tagtcgatta
     2161 tgcgcgcatg acatttacgc aattcctctg tgccagtcat gccagcgtga tattgagcgt
     2221 gatcgcgata attgggagaa gacgcacggt aggctggcga tgcatcaacg attgttcttt
     2281 gattacgcgc ttggagtcgg cgctatcaca agttactcgt cgagcgttag ataaaattgc
     2341 tctaatgtgt tgctatttct ttaatcgagg gtattatatt ccacgttgat tagttgacat
     2401 gggctaatca gtaggtgaca ggatgttacc gtacagcgta tacgctgggg taaaaagatg
     2461 catagccgtg aagataagag cctgtacttt gataaacgtg cttatgccaa cgttcaagcc
     2521 gcagaggccg taaaatctgg tcgtatgaga ctggataagg gtaatgaaac tattgaggaa
     2581 gcgtcgaaaa tccctgtagg gattaactcc gcaggtcaat ggaaggtgat gagtaaggag
     2641 gatatgaaga aaaaactcaa cctgcactca ccagaccatt gggatacata ttgtttcgct
     2701 atgctggcgg attatgttcc ccaggacgaa gtgcttagcg tcgaagacga agcgcaggtt
     2761 gatgaagctc tggcatggct taatgaataa acacttgctc taataaattg tttttttgac
     2821 tgcagatgtt acattgaacc tgacctcttg cgccttgagg cattttcggt ttatgcttat
     2881 caggcacctc attaaaacgg gtgccgggat tgagaccccg gataatgtca aaggcgacac
     2941 agacgccgaa agcgtctttt tttgtgtcat gccatcgcac agccatacgt agcgtttagc
     3001 tccgaaatca atggtagtgc tggctgggct gccgaaaggc aggccggttc cctttgacgc
     3061 cggtagtctc aacccagtca gtgctaccgc cattgagatt gagacctcac gcggtagctc
     3121 cttaacttag tcaaaggagg ctgccattat ggctactgtc cctaccccaa ttcatcctga
     3181 atttatctgg cgcttttact cctgccaaaa acgtcactat cacttcgtta ttgcaccgac
     3241 agaagatgag gcccgttctc agcttcctga cgctccctgc attttctctg cccgcttttc
     3301 aaccgatttg cgcaattctc tcagttactg gtgcctccct gttaacgctt ctgctcagga
     3361 gggactatga gaacgtcatt agttacccgt gaagagatga tcgaggcaat tgaacagcac
     3421 actgcctgta tcagtaccag ggatataccg ggcgttattg ccaactactt catgatcacc
     3481 aaacaacttt accggagaaa ggacaagaac gcagttcacc gcatcctgct gtctgatatc
     3541 cgcgaatacc tgctcgaaca ggggcatctg aattacgcaa ccgtcgcagc cgaagcacgc
     3601 aaggaggcac acagaatgaa agctactaac gttaaatcag aaaaagttca tgcaccttca
     3661 gttcaggaat cagagctggt ggttgttcaa aatcagtcgg atgaaattcc cgttctggag
     3721 tggcagggag tgcgtgtcgt aacaaccgaa actcttgcta aggggtatgg tacagaagcg
     3781 attcgcattc gccagaatca tcatgagaac aaagtacgct ttgttgaagc gaagcacttt
     3841 ttcaaggtta ttggcgatga gctgaaaaat ttgcgggtag ctttaaacta ctcacaaaat
     3901 ccagtctccc ccaaagcgcg ctctctcatc ctctggacag aacgaggagc tgcccgtcac
     3961 gctaaaatgc tcgaaaccga tcaggcatgg gcattctttg aaaaactgga agacagctac
     4021 ttccgacaaa aagaacagca accggtcgca atcccccaga cgcttccaga agctctgcgc
     4081 ctggctgccg agttagctga acaaaagcaa cttctggaac agaaagccca ccagctaaat
     4141 cagcagctgg tggccgccgc ttctaaagtc gattttgccg accgggtatc agtagctaaa
     4201 gggatcctga ttgggaattt tgcaaaggtc gttggactta aacaaaatgc gctgtttgcc
     4261 tggttacggg agaacggcat tctgatagcg tccggaggcc gtaaaaatgt accgttccag
     4321 cagtacatca acgcggggta tttcacggtg aaagaagtgg tgctggatga tgaagatggc
     4381 taccagatac ggttgacgcc tcaattaacg ggtaaaggcc agcagtggtt gacgcgtaaa
     4441 ctgctcgatg ctggcttgtt aaaaccggtg gcggctgaat aatggaagaa tgcccggttg
     4501 atgccgggca taatttattg cgcgctttcg gggttgtcgt ttactggctg ccccttcttg
     4561 gttttacggc tgcgcgtaac tgatgcggct gacttaacct ttttctcttc gcgagtgatg
     4621 gcaatttgtt tttttacatt ttcaatatct gccaggcgat atatttttgc ttgcggccag
     4681 cggtcgcaga tgatcggttc tatggagtca taaaggctaa attttgcttt ttcgaattca
     4741 ccgttgatga taattccatc acggagagtt tcatcgcaga taaacacgcc acacagtggc
     4801 acatggtaac taactgattt accatcattg tagttagggc tactggaaat gtagtggacg
     4861 cgcagcattg tttcgctaaa gccgtgtacg cgcatacgga atttttcatc ctccgggtac
     4921 tgcttcatta gctcttttgt tgcttccagg ttctctatgt atttcgcact gtgctcattg
     4981 atccccgcgc ttttttggat gcgaatgtcc ttatcaatca gatgaataat gcggccagcg
     5041 gtcatgttga cgctgttcac agcttctgtc tgataagttg taaccttacg cacaccgcga
     5101 aggatgttag gcactggata taaaatagtc tttgggatat tgaggtctgg gtactgttcc
     5161 agttcccgcg ccattaaagt ccatttatca atttcagcct gaatgctgtc agtttctttg
     5221 aacggtagaa cgacaaccgg gcgtacagga cgaccgtcgc tggctgtatc aacgtgttgg
     5281 gcgcgtgcaa cagctttttt tagaaagaga tccctgaagc tgacgaactc ctggtacagt
     5341 tgttcgccgt agacataatt tatcattgat cctcctccag aattgacatg gtcaataacg
     5401 cccggctgag aaaaccggtc attactgacc tatattatag agggatcaaa cgaaaataat
     5461 agatttatta gtgcatttat tgtgagtcta actggttagt tgccatgaga tattcgattg
     5521 tgtcagtgag gtcatccagg tcgtcttggg tgatgcggta ctcctgattg gatatctttg
     5581 agtagtgttc agcaatggcg cgggctgcgt cggtttcggc agggtctaca gataaagcgt
     5641 tagagcaatg tctaacgtcg tcgatggttg gttgaatgaa agccataatt atgcctcact
     5701 gtattgacaa cacagagcct gaagctctga cctactgttt cacccatgat ccatgctggg
     5761 gtaatctaac aacattgcgc tgtgtgtaag atgagcaatg catagggaag gtgcgaacaa
     5821 gttcctgata tgagatcatc atattcatcc ggagcgcatc ccagagggac atcatgagcc
     5881 atcaactcac cttcgccgat agtgaattca gcactaagcg ccgtcagacc cgaaaagaga
     5941 ttttcctctc ccgcatggag cagattctgc catggcagaa tatgaccgct gtcatcgagc
     6001 cgttttatcc caaggcgggc aatggccgac ggccctatcc gctggagacc atgctgcgta
     6061 ttcactgcat gcagcattgg tacaacctga gcgacggtgc catggaagat gccctgtacg
     6121 aaatcgcctc catgcgcctg tttgcccgat tatccctgga tagcgccctg ccggatcgca
     6181 ccaccatcat gaatttccgc cacctgctcg agcagcatca actggcccgt caattgttca
     6241 agaccatcaa tcgctggctg gccgaagcag gcgtcatgat gacccaaggc actttggtgg
     6301 atgccaccat cattgaggca cccagctcta ccaagaacaa agagcagcaa cgcgatccgg
     6361 agatgcatca gaccaagaaa ggcaatcagt ggtactttgg catgaaggcc cacattggtg
     6421 tcgatgccaa gagtggcctg acccacagcc tggtcaccac cgcggccaac gagcatgacc
     6481 tcaatcagct gggtaatctg cttcatggag aggagcaatt tgtctcagcc gatgccggct
     6541 accaaggagc gccacagcgc gaggagctgg ccgaggtgga tgtggactgg ctgatcgccg
     6601 agcgtcccgg caaggtaaaa accttgaagc agcatccgcg caagaacaaa acggccatca
     6661 acatcgaata catgaaagcc agcatccgtg ccagggtgga gcacccgttt cgcatcatca
     6721 agcggcagtt cggcttcgtg aaagccagat acaaggggct gctgaaaaac gataaccaac
     6781 tggcgatgtt attcaccctg gccaacctgt ttcgggtgga ccaaatgata cgtcagtggg
     6841 agagatctca gtaaaaaccg gaaataacgc cagaaatggt ggaaaaaata gcctaaatag
     6901 gctgattcga tgtgtttgcg ggaaaaaaat cggcccagat ccgcgaaatt ttaatcagcg
     6961 agtcagcttg ggaagaaatg acctgcttat tcgcaccttc catagctgta atgccgttgt
     7021 ataaggtttc cctgtttgct catttccttc tgagccgctc tacaacgctg aagacacatt
     7081 aaatagtgaa tccaaagtcg tattacgtaa cggcggcaaa actataattt attagagcaa
     7141 ttgtcaaaca actatgaaaa acaatccagt ttttggctgg tggagtggga tttttctctc
     7201 aaaatttatt gctctaataa ttcttgattt ttatgcgcag ctggacgtaa actcctcttc
     7261 agacctaata acttcgtata gcatacatta tacgaagtta tattaagggt tattgaacat
     7321 gatcaattta cctgtaaatc catacagttc aataccttat caggtcaaat agtgatcact
     7381 tgatcatttg atcaagtttg cgctacgtaa aatctgtgaa aagttggcag tgttagtgct
     7441 ccagatttcg cgtagcgcac ttagcaccac caatcaatca gaggtgaaaa atgggatatt
     7501 cagctgctaa agtgtccact catcttgagc ttgagaaaaa ccgtggttac tggcgggcaa
     7561 aagggtttga tcgtgatagt tgccaactgt cattatcgcg cggtgaagag aaaatagtac
     7621 gcacgcgcgg tcgctggcgt ttctatggcg agaaccataa acaggtaaag gcagagccga
     7681 tcctgtacac tttacttaaa accattatct gagtgttaaa tgtctaattt actgaccgta
     7741 caccaaaatt tgcctgcatt accggtcgat gcaacaagtg atgaggttcg caagaacctg
     7801 atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc
     7861 gtttgccggt catgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa
     7921 cctgaagata ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtgaaaact
     7981 atccagcaac atttgggcca gctaaatatg cttcatcgtc ggtccgggct gcaacgacca
     8041 agtgacagca atgctgtttc actcgtcatg cgacggatcc gaaaagaaaa cgttgatgcc
     8101 ggtgagcgtg caaaacaggc gctggcgttc gaacgcactg atttcgacca ggttcgttca
     8161 ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct
     8221 tataacaccc tgttacgtat agccgaaatt tctaggatca gagttaaaga tatctcacgt
     8281 actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgct
     8341 ggtgtagaga aggcacttag tctgggggta actaaactgg ttgagcgatg gatttctgtc
     8401 tctggtgtgg ctgatgatcc gaataactac ttgttttgcc gtgtcagaaa aaatggtgtt
     8461 gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca
     8521 actcaccgat tgatttacgg ggctaaggat gactctggcc agaggtacct ggcctggtct
     8581 ggccacagtg cccgtgtcgg agccgcgcga gatatggccc gcgccggagt ttcaataccg
     8641 gagatcatgc aagctggtgg ctggaccaac gtaaatattg tcatgaacta tatccgtaac
     8701 ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatta gccattaacg
     8761 cgtaaatgat tgttctaatt ctttgatatt tatggtgaca tatgagaaag gatttcaaca
     8821 tcgacggaaa atatgtagtg ctgtctgtaa gcactaatat tcagtcgcca gccgtcattg
     8881 tcactgtaaa gctgagcgat agaatgcctg atattgactc aatatccgtt gcgttccctg
     8941 tcaaaagtat gcgtggtgct gaacatttcg tgatgaatgc caccgaggaa gaagcacggc
     9001 gcggttttgc taaagtagtg tctgactttg gcgaactctt ggataaggtt aacaatgtcc
     9061 tttcaatcag ttcagcaagg tccaaagcgt taacagcttc catgatgaaa taaaaaaagc
     9121 ctggcaagga accaggctgc acaaaagagc gggtttgtgt tccgcatcca atcaatcaga
     9181 aaggagtata gcacatagat actaaagtga aaaaatgtga ctgaagataa acaatataac
     9241 attgctctaa ttgattgtta taattgagcc gtggcgttag tcaactacga agacgtggcc
     9301 attacctact taaccagttc cgccagggca gcagagtagg tttcatctcg gatgtaccat
     9361 gcctgcatgt tttccagaga agcccgcgag gttcccggac aggactctgc cattgcgata
     9421 gattaaatgc aataacctaa aagcgataac gtggtggaaa aatgttcatc agaacttatt
     9481 taagggcatc aacggaagat cagtttgccg atcgggcaaa agagatgctg gggcagtttg
     9541 ttcaggatcg gggacataaa atagccagtt actaccggga gaacataagc ggtacaaaac
     9601 tcgaccgccc ggaactggga cgcttactga tggacagcca ccgcaatgat attttactgg
     9661 tcgagcaaat tgaccgcctg acccgtctga gtaacagtga ctggattacg ctgaaaaagc
     9721 agatagaaca acacgaactg cggattgtcg cctggatgta tccacctcat ggcaggcgct
     9781 gtcagataaa gacccttcgc aggctgaccc aatcacccgc gccgtgataa ccgccatcaa
     9841 taacatgctt atcgacctga tggccgcgat gtcgcataag gactggctga gccgtcgcca
     9901 acggcaacaa cagggaattg aacgggctca tacgctgggt aaataccggg gataacaggc
     9961 cgatcaggaa cggcatcaga aagtcctgta ctaccggcag gtcaagaaac taagtatcca
    10021 tgaaacggca gatgcgacag ggtacagtcc ttcacagata tgccgcattc aagcccttta
    10081 tagagagcct gaatcggaat aaaaagtagc ccaggcgtta atggattacc tttccaggct
    10141 aagctcattg ctcctgtcag atacgcagcc ctgcctgtaa taatacagac tgggcaatgt
    10201 gttgccactc aggctgacaa ctgtcggagg atatccgctg tattataatg agcccgtttt
    10261 tctatatatt tcccgtcttt cagagtgatc ataaacatca gagaaaagcg cactttcctg
    10321 ccgctggcag gatgcccaag gaattcacgg gtctgcacgc cttcaaagat aagatagcag
    10381 gcgactttat cgtctctggc aaagatttcg tggatacgca ttgtaaatcc cggaaaagca
    10441 tccatatggc ctttttcctg tacgataaac tgctcactgt tcagcggtgt atccacctga
    10501 gaatagaatt taaaatccgg atggcagagg tttttcacct catcatattt attttcttca
    10561 atcagacgat aaaacttttt aaccgttttg atattttctt caacagacat attgcacctc
    10621 atttttcagt ttctttacct gccggaatat tgccttccgg cgttccgtta aatgtttttc
    10681 gccattcact gggactgact cccatccgct gccggaatat ctgccggaaa gtaatggtgg
    10741 actgaaagcc tgccagttcg gcaacccgct ccactgcaat atctgtactt tccagcagtt
    10801 cctgactccg ccggagacgc tctgtggtga tccactccca gaccgtcatt cctgtggccg
    10861 aatgaaaatg gcgtgtaagc gttcttcggc tcatggaggc taaatgggcc agagtgtcaa
    10921 tatcatgtgg taatcccaga tttgcggaca ggtattccag cagctggttt atgcggttat
    10981 cggacgtact tttgggaata agcagttgcc ggtactgtgc ctgtccgcct tcccggtaag
    11041 gaggagcaac catccggcgg gctatttgat tagccgtata tctgcccagg cgttgccgga
    11101 tcacatggag acaacagtcc agtgctgcgg ctgtaccggc tgaggtgata atccgctcgt
    11161 cctcaacata aagcacgttc acgtccagat gaaccgccgg gaaaagcatc tggaaatccc
    11221 gttcagcctc ccagtgcgtg gccgcccgtt taccatccag cagaccggtg taagccagaa
    11281 caaaactccc caggcacagg cccaccacct gagctccatt tttccaggct gcaaccaggg
    11341 cactgagcag agtttcagaa ggcctttcat ggatgtgttt ccagaaagga ataataacga
    11401 tatcggcctc tgccagtgca tcaagcccgt attctgccct catcgccgta ccttcagcag
    11461 accagataag cccgggctct tccgcacata cccggcggct gaagagagcc ttcccttcca
    11521 gtatctcccc gaacaggatt agtggaacgg agacatgaaa tgtactgaac ccttcggtga
    11581 tcactatagc gacagtgatg gtggacatgc catggcctcc gggttaaatc gtaactgatt
    11641 caccatcagc aggagcggca actgacgcct ggatctggtt atcgcgggca tattccagca
    11701 gttcctgacg ggtcagcaga cagtggttta cggcctccat gtgcgttgcc acaatcagtg
    11761 cctctggtac gatacgatgg gctttcagga catcttccct gccgaaaata atggggccga
    11821 atccgagaac atgcgcccag cccgtattca taataacgac gccaggattg aaccgacgta
    11881 aattatcctc tacagccttg atccagatgg tatccccgac cagatagagc gttttttcgg
    11941 acggatgctg gaagaccacc ccacaggcat ccccaaggat tgctgcgagt tgcgggttag
    12001 cataggcttc gtccgaacca tgctgacaaa cggttttaat aagtgaaatt tcgccaaatg
    12061 tgcttttgtc agacagaata acaaggttgc tgaagccctg ggaacgcagg atcttttcat
    12121 cattttcatt ctgaacataa atcagtttat ctttcgggat gtgcgcaaca gccgcttcat
    12181 cccagtggtc agggtgtgtg tgggtgacaa taatggcatc cacatccagc agtacatcaa
    12241 ggtcaaaagg cagctcagtc atcgggatgc ggatatctga ccgggccgtt ccttcgaatc
    12301 ccggataggc ttcttttttc gcaagcatcg gatcgacaag aaatttttta ccagcgtaag
    12361 aaatgagttg tgtggcgtta cgtacctgga tgatcttcat tttagcaagt cctgtattgg
    12421 ttaataacca tcagagccat atcatgatgg cctaatgtga taattatgat taaatcaggc
    12481 ctgcctgcca ctgaaataat gccatatttg gatgatatca ggccattctt ctggctctca
    12541 ggtttcagga atatgtctgc tgaaagcggg tacagatctg acgcattgaa gggaagtatc
    12601 gtcctttaag gcttattgat gtcagccagt acaaaaaatc atcttaacgt acaatatctt
    12661 ccgtttccca aacggagccc accaacgacg gaggaacggc gcatcgccaa tgctcttggc
    12721 gctctcccct gcattgcctg ctatatgcct ggagtaatat ctaatgaggt gtctctgcac
    12781 catatcgccg gtcgtaccgc gccgggttgt cacaaaaagc aattgccact ttgtagatgg
    12841 caccaccagc atgcagcacc ggctgaagta agagaaaaat tcccctggct ggtccctgtt
    12901 catgccgatg gcgtggttgg aggcaagaaa gaattcaccc tgctgaacaa gtcagagatg
    12961 gagttgctgg ctgacgccta tgagatggca aatatcatgc actaatatat ttattatttt
    13021 taaaatattg gggcgcgtat gcccctgttt gttaatgttt tttgtcctct tccagacgag
    13081 caacttttaa ttgtttgtcc gtgatagtaa gcaatgcaac cccttctttt tcgtacaaat
    13141 aaccagctcc attaatcaat aatacaaacc gatcctttcc tcggaatact tcataatact
    13201 taccagcacc acaggtttta gtttcaatgc agtccaaacc agagattgca gccttatcaa
    13261 agatatggtc cttcgtcgca acaggtttcc catactcttt tgtaagcatt tcatctaatt
    13321 gcataaaggt atatttgcca acttttgtcg tcatgctcaa ctcaaccttg ccattaatca
    13381 acattcctgt ggtatgtggt tcctgatcgt caggtgacga tttatcgtat aatgcaaaaa
    13441 gttctatgtc tggagtccgt ttttcaaaag tggcttcgtc ctgttcttca agataactct
    13501 tgtcttcacc gataagtgag agtttatcaa tctctgactg gttacaccca gttaacatta
    13561 gagccatagc aatagaagcg ataatgttga aactacgatt tttcattaat caatctccgt
    13621 gatttgtggt catcaagacc ggacgcagga atagcccaga ctctactact gcaaccatat
    13681 aaaaataata aaaatattgg tttatagcat ttttacagat aaaaaaattg acatctgaca
    13741 aattacttct gccaaaatca catcaagccc ggtacggatg gatccctttt caaatattcc
    13801 atggacggca cagtctgagt actgggcgca ccttcagttg tattgctaag ccgccgctgg
    13861 tggcttttct tttttgtagg ggcgctatgg ataagaaaat atgcgttgtt tcaatgagcg
    13921 tcggcaaacc ggcgtcaatg actgctgcat ggatcaacaa tgagctgata atggctgagc
    13981 ggactagcta ccctgaacgc cgccgcgata tggaactcca gctgctgcgt gaattgcgag
    14041 aaaaagagga aaagggtttt atcgtgctgg tggaagagga aaacagcttt attaccggtc
    14101 gagttggcca gcgtgtaagg ttgcgcgatc ccttcatgaa cggcaggcca gtactgattg
    14161 aagcaatgca gatttacaag gaactagaac gccaaaaagc gatcaagtta ccgcgcaagg
    14221 aatccggcaa atacatcctc caccaaagca tcttcgattc cgaacacgac aaaaaaggcg
    14281 atgaattttt caacatcaac tggagcgaaa taacgacaga gcacgttctg acgttgttat
    14341 gcagcttcgc aacggaatat aacaacgtgg caagcgccga ctacatcagg gcaatggcag
    14401 gagaagttga gtccaataag gaaccatcgt tactaagtcc tctgattaac ataattcgcg
    14461 gtacccaatg tctagcagac aagcgagttc ctcaaggctt acttaccggt aaagaaaact
    14521 atttataatt acttacgtta gtagcaaata gagatttttg ttgtggaaca aaaatataca
    14581 ttttacaatc cagtaacttt gggtggagga ttattacaat tatgaatatt aagccgctga
    14641 aaattagcaa caaggtcatg aagcctcata aagcatcatt aaaccctgat cttaccgcta
    14701 ggaatgtact tacatacagc cattgggatt ttgtggaact ttggttaaag cggaacgaaa
    14761 aagatgaggc tcttttttat tgggagcagg caaaagtatt taatcaggcg gccaatggtt
    14821 tacccaacca atcagcccct cttttgcact attattcatt catgaatgca gtaaaggcgt
    14881 tactttcatc tcgcaatata aacttcaaac agcatcatgg tgttgctagg ggtgataatg
    14941 cagcccaaat taataattta tctgacataa aagtaaaaat aaaaaatgag ggtatacttc
    15001 cttctttttc aaaatatata gacggcgcct ctcatgaggg ggtgtacaat ataaaaagcc
    15061 ttttttctgg attgccatat attcatcgta catattgcct tacttatgat gtaactgatg
    15121 atatatttat tccactgatc gatgccgagt tcgttttgaa tgagtcagac aatagtctgt
    15181 ttttcactgc gattttatcc aaggattttc ggttcgacac agttttcgac attctaccgc
    15241 cagcgttcga aatgtttgaa atagaaaagt acaaaataag atcagtagaa tcattacatg
    15301 attttaatga aaatcaaccg ttcgccatgt cgcaattaac tgaatttcac aaaaaaataa
    15361 gaagaaatct ttactatatc aatggcgcag agactctttg gtatttcaaa agtaaaaaca
    15421 aacaatctga gcatactcct gtttcaatat cgccattggt tataacccta gcagccatgc
    15481 atagattaag tgagctatgt cggtatgacc cacttaagct aagacagtta ctaaatgaaa
    15541 aagaaaactg gattattgct gaatttattc aacaatctcc gtctcagttt atagatgcaa
    15601 tttcatctga aattactggg caccaatttc ttatcccaaa cgtaaggtct gcttcttaat
    15661 gaatatatat atcgcatgcg ctttaactca tgtcccaagg gaaattttcc atgagtacag
    15721 taattggatt cacagccttg caaaaggtct atctcaaaat aacaatgtga agtatgcttt
    15781 gataaatagt gacccggcat tatcaaagcg tcctgaaagt aacaaatcta gactttgtta
    15841 catttgggac agagatatgg ttgaaaaatc tgatgtaata attgcagaat gtagctttcc
    15901 atcaacaggg ttaggaatag agttacagat tgctgaacag aataatattc ctgtaatcat
    15961 ctgttataaa gattatggga taaataaaac taaaaccatt gaatacgtga atccagatga
    16021 aaccacccat aatctacaag ttggcgaggg tttcatttct catatggtgt taggactacc
    16081 aaacatcctt gatgtcatat tatgtaaaga tattgataac acatgcagga agataaaaat
    16141 actgttagat atgattaacc ataattaaaa attaacacca actcaagatg tctaaatcag
    16201 cccggtatta accgggctag acaaacttaa tcagcattca ggagcaatgc gttatctatg
    16261 atgatctgct cccattcttc gaatgcccga tcgcggacgc cctggggaac gctgttagtt
    16321 ttgaaatcga cgaccgtccg ccatttcccg tccggacggt acatgcgcag agctttactc
    16381 cccccttccc tgcgcacctc aacgttatgc ttgtcagcaa actcttgtaa tgctcgtagc
    16441 gtcccatgct ttactgtgta gtatcgcttt ttcaagtttt ctctccagcc tgtgctaagg
    16501 cttcaacttc caaatcgtaa gactcaaact catagtcctg gtcatcaacc tcttcagaca
    16561 ctggcagtaa atgccaggct gagtatatct gaccattatc aaaacgctcc tggctgtaga
    16621 gcgtcgcggc tatgagcgtc agcgccggtc ggtcataacg gtaaattttg cgaacgtcac
    16681 ggtcaacgag acgaccgaaa ttaccataac cgcgctccag taataatttt ttaatttccg
    16741 gccagtatgg gccatagctg cggtacagac ggggattttt cagtaatcgc ccgcgtagcc
    16801 ctgacaggaa gaaatcaacg tattcgtctt ctgtctttcc taacaacgct gtacggagta
    16861 ccgcctcaag atatgtttta ttcggtttta ttgtatcaga tagtgtggcc atattatgcg
    16921 acgcccggcg aaccgggcgc tcctgttatg cgtattgttg gatgacggcc agaacgtccg
    16981 ccacgttgtg ttttgtctcg ataatccacc agttacccgg gaaatcgctg ttcttcgcct
    17041 tcgctggcag ccagcgagcg ccgaatttcg ccttgattgc gtctttagcg cggaaaagaa
    17101 cgcctttcat gccggaggct tcctgaagcc caaatacctc gccagcggcg aattttggtg
    17161 cgtacatcat cttcaggtcg gcggtggata cgcgataatt cagaccaaga gactgagcta
    17221 tgccggtggc atcaccttgt aatgatgata actcttcttg tttctcgttt ctggcggcaa
    17281 tttcttcctc cgtgatgttg ccaagggcca ggtttatccg atcagcgtct gcctgtttct
    17341 cttcatcggt gcgaccggca agtgccgtgt taattctctg caatatctcc acatgattct
    17401 tgcgcatgct gagcaattcc ggcgtaacct cgttaaggtc caccagccca aggatggcaa
    17461 ggtcagaaaa cattgatacc aggttgtagg tcatgcgata gctgagttgg ccataggctg
    17521 atggcaactg caccgaatcc attttatagg catccataaa tttagagccg tcgtttacga
    17581 catccgcaat tgcaggtgtg attttccctg tggtggcggc ctccctgatt gctgttaccc
    17641 acgattgagt cagcgcggcg actgcatgat tcagattggc tttccgttct gctgcaatgc
    17701 gcgcgcttgc agcgtccatt gcctgcttga tctcgtcttt attgctgtaa atgccaatgg
    17761 tgccaaactg tgctgtggtg atctcaaagt ctgacgcccg gaactcatta gtaccgaaaa
    17821 tggcattggt gacctcaagt tcagaatcac cgttacgggt agccccctgg cttgttttct
    17881 ccggcattct ggcgatcgca tccgctattt tctcctgaat tgcttcaggg gatagcgtat
    17941 ctccgtatga cgcgattaca tcgccataat tggagccaaa cagttcagtc aggaatactt
    18001 ctgccaaacg gacctggcgg ttattccctt ccgacatcat accaagcacc catttagcaa
    18061 ttgacgactt cagggcgccg tcacggcgat ccggataaac cgcatgcttc aacgggtccg
    18121 tataggtacc aacaaaatca atgctgtagc ctgactctgt agtctgaacg ccgtatgagt
    18181 cagtgatttt gatcatgccg cgctgctgga aacggtagaa atcgtcacag gaaatgatgt
    18241 cgttaatccc ggcgatggag acgccaccac tgattttctg cataacagca tcttcatcgg
    18301 gagttacatc cacctgttta tccagcgtct tcacatccca gttacccgat ttggtgcctt
    18361 tgaaggtaaa gatgatctcc acgtctgcgc gctggctgtc gaagtccagc gacttaatgc
    18421 gaacgatatc accggcacaa tcgtagtatt ggcctacccc cgccgggcac atgtatggat
    18481 cacgtcggca accttcagcg tttccgataa gctcaagccc cgcctggttg gttcgcacat
    18541 tgccattacc catcacgatg gtaatcatca ccgcgatagc gcaaattgca ccgcctcctg
    18601 cggctgtttt tcccttcata aagacctcat aagcgaattt ttacgctcca ggacaaacac
    18661 ccattcacag ccaataccga ctgactcgat ccctttcgaa ggcacaggat aatgcaaatc
    18721 acttgttagc tacgtttcaa agatatacat tattgctcta attaatttgt tttattgggt
    18781 aagaaaagtg gcacaacgcg gtgtaaacaa agtcatcctg attggtaccc tggggcaaga
    18841 cccagatatc aggtatatac caaatggcgg agcggtcgga agactaagca tcgcaacaaa
    18901 tgaatcatgg cgcgacaagc aaacgggcca acagaaagag caaacagaat ggcatagagt
    18961 tgttttgttc gggaaacttg ctgaaattgc gagtgagtat ttacgaaaag gttctcgggt
    19021 ctacatcgaa gggaaactta aaacccgtaa gtggacagat gacgccggtg tagaacgtta
    19081 cacgacggaa attatcgtca gccagggcgg aactatgcaa atgatcggcg cccgccgcga
    19141 cgattcacag tcctcaaatg gctgggggca atcaaaccaa cctcaaaacc accagcaata
    19201 cagcggtggc ggcaaacctc agagcagcgc caataacgaa cctccaatgg actttgacga
    19261 cgatattcct ttttagagtt aacttgtgag ttatttgtaa atagttcata cacaggcccc
    19321 tgttaataca ggggattttt atttctagta actataagag gtgttaatta ttaattgaca
    19381 atctttagtg tttcgcgcac cttgcaagta tccaatattt actgattgtg gacgcataaa
    19441 gaagatccac agcgttacaa atttgtattt ctttcttcag caattaagca ggcgacttgc
    19501 tctatcctgc ccaaaggaac acgtatgaca cactgcaaag aaaatacttg gtatgagcgt
    19561 caagggtaca ataccacccg cccgaatgag ataaggtcgg agtatgaacg tgatctttcc
    19621 cggttaatcc actcttccgc ttttaggaga ttacaatcga agacgcaggt gttgggattg
    19681 ggcgaaagcg atttctaccg tacgcggcta actcactcta tggaagtagc gcaaattggc
    19741 ggcggtatct tgtctcagtt gtctaagcgc gatacagatg aggggggtaa attcttacct
    19801 gacccaagcc tgatgcaggc tatctgcctt gctcatgata taggacatcc ccccttcggt
    19861 catggtggtg aggtcgcact aaactactgc atgagatcct atggcggctt tgaaggtaac
    19921 gggcagacac tgcgtatact ctccaaactg gataagtaca ccgaaaccaa tgggctggat
    19981 cccacccgta gacttatttt aggcgtactg aaatatccag caagctattc agaggtcgtc
    20041 aatgagaggg cttacagcca gttacagcca agaagcaatg agtggttgtt taaatcatcc
    20101 gaatttaagc ccccgaaatg ttacctggac tctgaggaga acactgttag attcgcactc
    20161 caaccttttg atagtgatga tgttaagtta ttcaaaacgg taaaaactac cgcgttgaat
    20221 aagcacaaga aaacaatata taaagcactg gatacaacga ttatggactt ggctgatgag
    20281 atatcctact cactccatga tcttgaagac gcaatttctc tgaagatgat cgacaaaaaa
    20341 atgtgggagg agcacttcga ggacaaatcc catttatttg cagcttgcca atgcgactcg
    20401 tttaatctaa aagccgatga tgtagctgaa aaactgttcg gcgaaagtta tctacgcaag
    20461 gagtgcatag ggacacttgt tcatctgatg atcactagcg ttaagcttga agtgcagaac
    20521 tctgatttca aaagtaaaat tttacgctat aaagtggcac ttcctgccgc ggtagaagag
    20581 ttacgtaaag ccatttttga gctggttagg aacaaagtta ttcagcacga aaacgtccaa
    20641 ctattagagt tcaaaggtca gaaactgatt gtggaactat tcaatgtact ggcaaatgac
    20701 ccaatacgtt ttctcccgac agaaactcga aagaaatata atcaagagga agcaaaaggt
    20761 gatgataaaa aaatgcgcgt catttgcgac tatgtttcag gcatgacaga tgattacgct
    20821 actcgactgt atgagaaaat atttgtaccg agaaaaggct ctatttttga tagactctga
    20881 ttttttctat tatatagtac gtagccgggt tccagaaccg cataaaatta gatctggata
    20941 taattcattt aattatctgt tatatgccaa acataacgat atataatagt ggattagatg
    21001 ttacaacttg acgcagaata aatagctttt attggcgcat gatgcaaacc ccgcaatgaa
    21061 gcggggtttt aactgtggta aatgagttat aatcttttaa tcaggctctc aatattagtt
    21121 ggttcaaagt aatgcttatt aacctctcca tttatgagga aaccgtcttt tagtagtcgg
    21181 tctagctcac gctctgcttc tttgttgaac atacctggat atattctctt gtacaactga
    21241 aatgtaccat tcatattcct gtaagcattc tcagcaattt caatggagat ttcatttccg
    21301 gcgttaaata caggaatgaa tatctcaaga atatcattct ttaacttctg tttaagtttc
    21361 tttttttcaa caatattaga ataaaagata cctaaaaaaa atgtgcagat accaggaagc
    21421 caattattaa cgaaaacctc agacaaagcg tcca
//
LOCUS       DAEVZA010000087        21447 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEVZA010000087 DAEVZA010000000
VERSION     DAEVZA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.087"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..122
                     /locus_tag="LAF92_003227"
     CDS             <1..122
                     /locus_tag="LAF92_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792184.1"
                     /translation="NIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            305..499
                     /gene="bfd"
                     /locus_tag="LAF92_003228"
     CDS             305..499
                     /gene="bfd"
                     /locus_tag="LAF92_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBI9792185.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            572..1048
                     /gene="bfr"
                     /locus_tag="LAF92_003229"
     CDS             572..1048
                     /gene="bfr"
                     /locus_tag="LAF92_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBI9792186.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1045..1512)
                     /locus_tag="LAF92_003230"
     CDS             complement(1045..1512)
                     /locus_tag="LAF92_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBI9792187.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDCLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1891..2202
                     /gene="rpsJ"
                     /locus_tag="LAF92_003231"
     CDS             1891..2202
                     /gene="rpsJ"
                     /locus_tag="LAF92_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBI9792188.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2235..2864
                     /gene="rplC"
                     /locus_tag="LAF92_003232"
     CDS             2235..2864
                     /gene="rplC"
                     /locus_tag="LAF92_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBI9792189.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2875..3480
                     /gene="rplD"
                     /locus_tag="LAF92_003233"
     CDS             2875..3480
                     /gene="rplD"
                     /locus_tag="LAF92_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBI9792190.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3477..3779
                     /gene="rplW"
                     /locus_tag="LAF92_003234"
     CDS             3477..3779
                     /gene="rplW"
                     /locus_tag="LAF92_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBI9792191.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3797..4618
                     /gene="rplB"
                     /locus_tag="LAF92_003235"
     CDS             3797..4618
                     /gene="rplB"
                     /locus_tag="LAF92_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBI9792192.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4635..4913
                     /gene="rpsS"
                     /locus_tag="LAF92_003236"
     CDS             4635..4913
                     /gene="rpsS"
                     /locus_tag="LAF92_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBI9792193.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4928..5260
                     /gene="rplV"
                     /locus_tag="LAF92_003237"
     CDS             4928..5260
                     /gene="rplV"
                     /locus_tag="LAF92_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBI9792194.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5278..5979
                     /gene="rpsC"
                     /locus_tag="LAF92_003238"
     CDS             5278..5979
                     /gene="rpsC"
                     /locus_tag="LAF92_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBI9792195.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5992..6402
                     /gene="rplP"
                     /locus_tag="LAF92_003239"
     CDS             5992..6402
                     /gene="rplP"
                     /locus_tag="LAF92_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBI9792196.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6402..6593
                     /gene="rpmC"
                     /locus_tag="LAF92_003240"
     CDS             6402..6593
                     /gene="rpmC"
                     /locus_tag="LAF92_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBI9792197.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6593..6847
                     /gene="rpsQ"
                     /locus_tag="LAF92_003241"
     CDS             6593..6847
                     /gene="rpsQ"
                     /locus_tag="LAF92_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBI9792198.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7012..7383
                     /gene="rplN"
                     /locus_tag="LAF92_003242"
     CDS             7012..7383
                     /gene="rplN"
                     /locus_tag="LAF92_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBI9792199.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7394..7708
                     /gene="rplX"
                     /locus_tag="LAF92_003243"
     CDS             7394..7708
                     /gene="rplX"
                     /locus_tag="LAF92_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBI9792200.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7723..8262
                     /gene="rplE"
                     /locus_tag="LAF92_003244"
     CDS             7723..8262
                     /gene="rplE"
                     /locus_tag="LAF92_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBI9792201.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8277..8582
                     /gene="rpsN"
                     /locus_tag="LAF92_003245"
     CDS             8277..8582
                     /gene="rpsN"
                     /locus_tag="LAF92_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBI9792202.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8616..9008
                     /gene="rpsH"
                     /locus_tag="LAF92_003246"
     CDS             8616..9008
                     /gene="rpsH"
                     /locus_tag="LAF92_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBI9792203.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9021..9554
                     /gene="rplF"
                     /locus_tag="LAF92_003247"
     CDS             9021..9554
                     /gene="rplF"
                     /locus_tag="LAF92_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBI9792204.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9564..9917
                     /gene="rplR"
                     /locus_tag="LAF92_003248"
     CDS             9564..9917
                     /gene="rplR"
                     /locus_tag="LAF92_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBI9792205.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9932..10435
                     /gene="rpsE"
                     /locus_tag="LAF92_003249"
     CDS             9932..10435
                     /gene="rpsE"
                     /locus_tag="LAF92_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBI9792206.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10439..10618
                     /gene="rpmD"
                     /locus_tag="LAF92_003250"
     CDS             10439..10618
                     /gene="rpmD"
                     /locus_tag="LAF92_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBI9792207.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10622..11056
                     /gene="rplO"
                     /locus_tag="LAF92_003251"
     CDS             10622..11056
                     /gene="rplO"
                     /locus_tag="LAF92_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBI9792208.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11064..12395
                     /gene="secY"
                     /locus_tag="LAF92_003252"
     CDS             11064..12395
                     /gene="secY"
                     /locus_tag="LAF92_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBI9792209.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12427..12543
                     /gene="rpmJ"
                     /locus_tag="LAF92_003253"
     CDS             12427..12543
                     /gene="rpmJ"
                     /locus_tag="LAF92_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBI9792210.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12690..13046
                     /gene="rpsM"
                     /locus_tag="LAF92_003254"
     CDS             12690..13046
                     /gene="rpsM"
                     /locus_tag="LAF92_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBI9792211.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13063..13452
                     /gene="rpsK"
                     /locus_tag="LAF92_003255"
     CDS             13063..13452
                     /gene="rpsK"
                     /locus_tag="LAF92_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBI9792212.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13486..14106
                     /gene="rpsD"
                     /locus_tag="LAF92_003256"
     CDS             13486..14106
                     /gene="rpsD"
                     /locus_tag="LAF92_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBI9792213.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14132..15121
                     /gene="rpoA"
                     /locus_tag="LAF92_003257"
     CDS             14132..15121
                     /gene="rpoA"
                     /locus_tag="LAF92_003257"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBI9792214.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15162..15545
                     /gene="rplQ"
                     /locus_tag="LAF92_003258"
     CDS             15162..15545
                     /gene="rplQ"
                     /locus_tag="LAF92_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBI9792215.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15652..16020
                     /locus_tag="LAF92_003259"
     CDS             15652..16020
                     /locus_tag="LAF92_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBI9792216.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16031..16456
                     /gene="zntR"
                     /locus_tag="LAF92_003260"
     CDS             16031..16456
                     /gene="zntR"
                     /locus_tag="LAF92_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBI9792217.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGNVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16512..16730
                     /gene="arfA"
                     /locus_tag="LAF92_003261"
     CDS             16512..16730
                     /gene="arfA"
                     /locus_tag="LAF92_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HBI9792218.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16727..17137)
                     /gene="mscL"
                     /locus_tag="LAF92_003262"
     CDS             complement(16727..17137)
                     /gene="mscL"
                     /locus_tag="LAF92_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBI9792219.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17267..18643)
                     /gene="trkA"
                     /locus_tag="LAF92_003263"
     CDS             complement(17267..18643)
                     /gene="trkA"
                     /locus_tag="LAF92_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBI9792220.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18665..19954)
                     /gene="rsmB"
                     /locus_tag="LAF92_003264"
     CDS             complement(18665..19954)
                     /gene="rsmB"
                     /locus_tag="LAF92_003264"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBI9792221.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20000..20947)
                     /gene="fmt"
                     /locus_tag="LAF92_003265"
     CDS             complement(20000..20947)
                     /gene="fmt"
                     /locus_tag="LAF92_003265"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBI9792222.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20962..>21447)
                     /gene="def"
                     /locus_tag="LAF92_003266"
     CDS             complement(20962..>21447)
                     /gene="def"
                     /locus_tag="LAF92_003266"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBI9792223.1"
                     /translation="IPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEGIGLAATQV
                     DIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVPRAEKVKIR
                     ALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVEKLDRLKAR
                     A"
BASE COUNT         5509 a         5129 c         5578 g         5231 t
ORIGIN      
        1 acaacatcaa aatggttgtt accctgatcc acccgatcgc gatggacgac ggtctgcgtt
       61 tcgcaatccg tgaaggcggc cgtaccgttg gcgcgggcgt tgttgctaaa gttctgggct
      121 aattacacgt taattagttt tgaattgaaa agggcgcttc ggcgcccttt ttgctgcccg
      181 taatttgagc tcgaaatatt tagtcgtaat tttgctcgct gaactatggc gcaaataaaa
      241 ctgtgctatt gtaatcataa ccattctcat ttacactttg cgcagaattt gaacgggagc
      301 aatcatgtac gtttgtttgt gtaatggtgt aagtgacaaa aaaattcgcc aggcagtccg
      361 tcagtttcat ccgcaatctt tccaacaatt gcgtaagttt attcctgtgg gaaatcaatg
      421 cggtaagtgc attcgcgccg cgcgcgaagt gatgcaggac gagttgatgc agatgccaga
      481 atttaaagag attgcctgaa gaccacgctt attttttgac atcccgctag ccccatctac
      541 gctctaatag tggaagcgga gggactataa aatgaaaggt gatgttaaaa tcataaatta
      601 tctcaataaa ctattgggaa atgagcttgt cgcaatcaat cagtattttc tccatgcccg
      661 gatgtttaaa aactggggcc tgatgcgtct caatgacgtc gaataccatg aatccatcga
      721 tgagatgaaa cacgctgata aatatatcga gcgtattttg tttttagaag gaatccccaa
      781 cttacaggat ttaggcaaac tggggattgg tgaagatgtc gaggagatgc tgcaatccga
      841 cctgcgatta gaactggaag gcgcgaagga tctccgtgag gctatcgcgt atgccgacag
      901 cgttcatgac tatgttagcc gtgatatgat gatcgagatc cttgccgatg aagaaggtca
      961 tattgactgg ctcgaaactg agctggatct catcggtaaa atcggtttac aaaattacct
     1021 gcaatcacaa ataaaagtta aagattaacg gcctgccagc aaactatccc aacctacaac
     1081 gaaacccgca gccgccagaa atggcccaaa cggcagcggg ttttttaatg actcttttcc
     1141 tctcatgagc agaccaacaa ctacggctcc gcaagcgaat gaggcagcaa ggaaaaccag
     1201 tcgtggcaag aaggtccagg tatgccaggc accaagggcg gcgagaaact tcacatcacc
     1261 atagcctaat ccttctttat gacgcagtat gcgatacccc cagtaaatga cagcgaatgt
     1321 gccgtaacca ataatcgccc cccataacgc atcggctaaa cagtcaggat tacaaacctg
     1381 agagaacaaa agacctgacc agagtaacgg gcaggtaaat ctgtcaggta acaagccatg
     1441 ctttgcatcc cataaaaaaa gtaggacaga taaacaagcg tacaaaatca gaaagggaag
     1501 ggtagcgtcc ataaaacatc ctctgtaatt ggagtatcac catcgaataa ctccggatgc
     1561 tcagcaacag agaaatcgct gatttgttaa cctggttccg gtgaaaaacg ctatcttcac
     1621 aaccttacaa atttcgtgga atacagctcg caatctgcgc gttgcgatta atttctaccc
     1681 gatgcttgcg tcaggctcag atttgcgtat aatgcgcggg cttgtcgtag ttgacagcag
     1741 gttcaatctg aacctcagtt agccgaattt ggctacctaa caatgctccc aatcggggag
     1801 ctacgtaaga acggttacac tctcccatca atcgtaatgg gtctgaggag taatcatttt
     1861 cgtttataaa ataattggag ctctggtctc atgcagaacc aaagaatccg tatccgcctg
     1921 aaagcgtttg atcatcgtct gatcgatcaa gcaaccgcgg aaatcgtcga gactgccaag
     1981 cgcactggtg cgcaggtccg tggtccgatc ccgctgccga cacgcaaaga gcgcttcact
     2041 gttctgatct ccccgcacgt caacaaagac gcgcgcgatc agtacgaaat ccgtactcac
     2101 ttgcgtctgg ttgacatcgt tgagccaacc gagaaaaccg ttgatgctct gatgcgtctg
     2161 gatctggctg ccggtgtaga cgtgcagatc agcctgggtt aatcaggtca ttgagcgatt
     2221 gagaggttga aacaatgatt ggtttagtcg gtaaaaaagt gggtatgacc cgtatcttca
     2281 cagaagacgg cgtttctatc ccagtaaccg taatcgaagt tgaagcaaac cgcgttactc
     2341 aggttaaaga cctggctaac gatggctacc gtgctattca ggtgaccacc ggtgctaaaa
     2401 aagctaaccg tgtgaccaag cctgaagctg gccacttcgc taaagctggc gtagaagctg
     2461 gccgtggtct gtgggaattc cgcctggctg aaggcgaaga gttcactgta ggtcagagca
     2521 ttagcgttga actgtttgct gacgttaaaa aagttgacgt aactggcacc tctaaaggta
     2581 aaggtttcgc aggtaccgtt aagcgctgga acttccgtac ccaggacgct actcacggta
     2641 actccttgtc tcaccgcgtt ccgggttcta tcggtcagaa ccagactccg ggcaaagtgt
     2701 tcaaaggcaa gaaaatggca ggtcagatgg gtaacgaacg tgtaaccgtt cagagccttg
     2761 acgtagtacg cgttgacgct gagcgcaacc tgctgctggt taaaggtgct gtcccgggtg
     2821 caaccggtag cgacctgatc gttaaaccag ctgtgaaggc gtaaggagat agcaatggaa
     2881 ttagtattga aagacgcgca gagcgcgctg actgtttccg aaactacctt cggtcgtgat
     2941 ttcaacgaag cgctggttca ccaggttgtt gttgcttatg cagctggtgc tcgtcagggt
     3001 actcgtgctc agaagactcg tgctgaagta actggttccg gtaaaaaacc gtggcgccag
     3061 aaaggcaccg gccgtgcgcg ttctggttct atcaagagcc cgatctggcg ttctggtggc
     3121 gtgacctttg ctgctcgtcc gcaggaccac agtcaaaaag ttaacaagaa gatgtaccgc
     3181 ggcgcgctga aaagcatcct gtccgaactg gtacgtcagg atcgtctgat cgttgtcgag
     3241 aagttctctg tagaagcgcc gaaaactaag ctgctggcac agaaactgaa agacatggct
     3301 ctggaagatg tgctgatcat caccggtgag ctggacgaaa acctgttcct ggctgcgcgc
     3361 aacctgcaca aggttgacgt acgcgatgca actggtatcg acccggttag cctgatcgcc
     3421 ttcgacaaag tcgtaatgac tgctgatgct gttaagcaag ttgaggagat gctggcatga
     3481 ttcgtgaaga acgtctgctg aaggtgctgc gtgcaccgca cgtttctgaa aaagcgtcta
     3541 ctgcgatgga aaaatccaac accatcgtac tcaaagttgc taaagacgcg accaaagcag
     3601 aaatcaaagc tgctgtgcag aaactgtttg aagtcgaagt cgaagtcgtt aacaccctgg
     3661 tagttaaagg gaaagttaaa cgtcacggac agcgtatcgg tcgtcgtagc gactggaaaa
     3721 aagcttacgt caccctgaaa gaaggccaga atctggactt cgttggcggc gctgagtaag
     3781 tcggaggagt aatacaatgg cagttgttaa atgtaaaccg acatctccgg gtcgtcgcca
     3841 cgtagttaaa gtggttaacc ctgagctgca caagggcaaa ccttttgctc cgttgctgga
     3901 aaaaaacagc aaatccggtg gtcgtaacaa caatggccgt atcaccactc gtcatatcgg
     3961 tggtggccac aagcaggctt accgtattgt tgacttcaaa cgcaacaaag acggtatccc
     4021 ggcagttgtt gaacgtcttg agtacgatcc gaaccgttcc gcgaacatcg cgctggttct
     4081 gtacaaagac ggtgaacgcc gttacatcct ggcccctaaa ggcctgaaag ctggcgacca
     4141 gattcagtct ggcgttgatg ctgcaatcaa accaggtaac accctgccga tgcgcaacat
     4201 cccggttggt tctactgttc ataacgtaga aatgaaacca ggtaaaggcg gtcagctggc
     4261 acgttccgct ggtacttacg ttcagatcgt tgctcgtgat ggtgcttatg tcaccctgcg
     4321 tctgcgttct ggtgaaatgc gtaaagtaga agcagactgc cgtgcaactc tgggcgaagt
     4381 tggcaatgct gagcatatgc tgcgcgttct gggtaaagca ggtgctgcac gctggcgtgg
     4441 tgttcgtccg accgttcgcg gtaccgcgat gaacccggta gaccacccac atggtggtgg
     4501 tgaaggtcgt aactttggta agcacccggt aactccgtgg ggcgttcaga ccaaaggtaa
     4561 gaagacccgc agcaacaagc gtactgataa attcatcgta cgtcgccgta gcaaataatt
     4621 ttagaggata agccatgcca cgttctctca agaaaggtcc ttttattgac ctgcacttgc
     4681 tgaagaaggt agagaaagcg gtggaaagcg gagacaagaa gcccctgcgc acttggtccc
     4741 gtcgttcaac gatctttcct aacatgatcg gtttgaccat cgctgtccat aatggtcgtc
     4801 agcacgttcc ggtatttgta accgacgaaa tggtcggtca caaactgggt gaattcgcac
     4861 cgactcgtac ttatcgcggc cacgctgctg ataaaaaagc gaagaagaaa taaggtagga
     4921 ggaagagatg gaaactatcg ctaaacatcg ccatgctcgt tcttctgctc agaaggttcg
     4981 ccttgttgct gacctgattc gcggtaagaa agtgtcgcag gctctggata ttttgaccta
     5041 caccaacaag aaagcggctg tactggtcaa gaaagttctg gaatctgcca ttgctaacgc
     5101 tgaacacaac gatggcgctg acattgacga tctgaaagtt acgaaaattt tcgtagacga
     5161 aggcccgagc atgaagcgca ttatgccgcg tgcaaaaggt cgtgcagatc gcatcctgaa
     5221 gcgcaccagc cacatcactg tggttgtgtc cgatcgctga gactctggag actagcaatg
     5281 ggtcagaaag tacatcctaa tggtattcgc ctgggtattg taaaaccatg gaactctacc
     5341 tggtttgcga acaccaaaga attcgctgac aacctggaca gcgattttaa agtacgtcag
     5401 tacctgacta aggaactggc taaagcgtcc gtatctcgta tcgttatcga gcgtccggct
     5461 aagagcatcc gtgtaaccat tcacactgct cgcccgggta tcgttatcgg taaaaaaggt
     5521 gaagacgtag aaaaactgcg taaggtcgta gcggacatcg ctggcgttcc tgcacagatc
     5581 aacatcgccg aagttcgtaa gcctgaactg gacgcaaaac tggttgctga cagcatcact
     5641 tctcagctgg aacgtcgcgt tatgttccgt cgtgctatga agcgtgctgt acagaacgca
     5701 atgcgtctgg gcgctaaagg tattaaagtt gaagttagcg gccgtctggg cggcgcggaa
     5761 atcgcacgta ccgaatggta ccgcgaaggt cgcgtaccgc tgcacactct gcgtgctgac
     5821 atcgactaca acacctctga agcgcacacc acttacggtg taatcggcgt taaagtgtgg
     5881 atcttcaaag gcgagatcct gggtggtatg gctgctgttg aacaaccgga aaaaccggct
     5941 gctcagccta aaaagcagca gcgtaaaggc cgtaaataag gagcgtcgct gatgttacaa
     6001 ccaaagcgta caaaattccg taaaatgcac aaaggccgta accgcggtct ggcgcagggt
     6061 acggatgtta gcttcggcag cttcggtctg aaagctgttg gccgtggtcg tctgactgcc
     6121 cgtcagatcg aagcagcacg tcgtgctatg acccgtgcag ttaagcgtca aggtaagatc
     6181 tggatccgtg tgttcccgga caaaccgatc actgaaaagc cgctggcagt gcgtatgggt
     6241 aaaggtaaag gtaacgtgga gtattgggtt gccttgattc agccgggtaa agtcctgtat
     6301 gaaatggacg gtgttccgga agagctggcc cgtgaagcat tcaagctggc agcagcgaaa
     6361 ctgccgatta aaaccacctt tgtaactaag acggtgatgt aatgaaagca aaagagctgc
     6421 gtgagaagag cgttgaagag ctgaacaccg agctgctgaa cctgctgcgt gagcagttca
     6481 acctgcgtat gcaggctgca agtggccagc tgcaacagtc tcacctgttg aagcaagtgc
     6541 gtcgcgatgt cgcacgcgtt aagactttac tgaacgagaa ggcgggtgcg taatgaccga
     6601 taaaatccgt actctgcaag gtcgcgttgt tagcgacaaa atggagaaat ccattgttgt
     6661 tgctatcgaa cgttttgtga aacacccgat ctacggtaaa ttcatcaagc gtacgaccaa
     6721 actgcacgta catgacgaga acaacgaatg cggtattggt gacgtggttg aaatccgcga
     6781 atgccgtccg ctgtccaaga ctaaatcctg gacgctggtt cgcgttgtag agaaagcggt
     6841 tctgtaatac agtacactct ctcgatacga ataaacggct cagaaatgag ccgtttattt
     6901 tttctaccca tatccttgaa gcggtgttat aatgccgcgc cctcgatatg gggattttta
     6961 acgacctgat tttcgggtct cagtagtagt tgacattagc ggagcactaa aatgatccaa
     7021 gaacagacta tgctgaacgt cgccgacaac tccggtgcac gtcgcgtaat gtgtatcaag
     7081 gttctgggtg gctcgcaccg tcgctacgca ggcgtaggcg acatcatcaa gatcaccatc
     7141 aaagaagcaa ttccgcgtgg taaggtcaaa aaaggtgatg tgctgaaggc ggtagtggtg
     7201 cgcaccaaga agggtgttcg tcgcccggac ggttctgtca ttcgcttcga tggtaatgct
     7261 tgtgttcttc tgaacaacaa cagcgagcag cctatcggta cgcgtatttt tgggccggta
     7321 actcgtgagc ttcgtagtga gaagttcatg aaaattatct ctctggcacc agaagtactc
     7381 taaggagcga atcatggcag cgaaaatccg tcgtgatgac gaagttatcg tgttaaccgg
     7441 taaagataaa ggtaaacgcg gtaaagttaa gaatgtcctg tcttccggca aggtcattgt
     7501 tgaaggtatc aacctggtta agaaacatca gaagccggtt ccggccctga accaaccggg
     7561 tggcatcgtt gaaaaagaag ccgctattca ggtttccaac gtagcaatct tcaatgcggc
     7621 aaccggcaag gctgaccgtg taggctttag attcgaagac ggcaaaaaag tccgtttctt
     7681 caagtctaac agcgaaacta tcaagtaatt tggagtagta cgatggcgaa actgcatgat
     7741 tactacaaag acgaagtagt taaaaaactc atgactgagt ttaactacaa ttctgtcatg
     7801 caagtccctc gggtcgagaa gatcaccctg aacatgggtg ttggtgaagc gatcgctgac
     7861 aaaaaactgc tggataacgc agcagcagac ctggcagcaa tctccggtca aaaaccgctg
     7921 atcaccaaag cacgcaaatc tgttgcaggc ttcaaaatcc gtcagggcta tccgatcggc
     7981 tgtaaagtaa ctctgcgtgg cgaacgcatg tgggagttct ttgagcgcct gatcactatt
     8041 gctgtacctc gtatccgtga cttccgtggc ctgtccgcta agtctttcga cggtcgtggt
     8101 aactacagca tgggtgtccg tgagcagatc atcttcccag aaatcgacta cgataaagtc
     8161 gaccgcgttc gtggtttgga tattaccatt accactactg cgaaatctga cgaagaaggc
     8221 cgcgctctgc tggctgcctt tgacttcccg ttccgcaagt aaggtagggt tactaaatgg
     8281 ctaagcaatc aatgaaagca cgcgaagtaa aacgcgtagc tttagctgat aaatacttcg
     8341 cgaaacgcgc tgaactgaaa gcgatcatct ctgatgtgaa cgcttccgac gaagatcgtt
     8401 ggaacgctgt tctcaagctg cagactctgc cgcgtgattc cagcccgtct cgtcagcgta
     8461 accgctgccg tcaaacaggt cgtccgcatg gtttcctgcg gaagttcggg ttgagccgta
     8521 ttaaggtccg tgaagccgct atgcgcggtg aaatcccggg tctgaaaaag gctagctggt
     8581 aattgtcacc aattgaatca cgggagtaaa gacagatgag catgcaagat ccgatcgcgg
     8641 atatgctgac ccgtatccgt aacggtcagg ccgcgaacaa agctgcggtc accatgcctt
     8701 cctccaagct gaaagtggca atcgccaacg tgctgaagga agaaggtttt attgaagatt
     8761 ttaaagttga aggcgacacc aagcctgaac tggaacttac tctgaagtat ttccagggca
     8821 aagctgttgt agaaagcatt cagcgtgtca gccgcccagg tctgcgcatc tataaacgta
     8881 aagatgagct gccgaaagtt atggcgggtc tgggtatcgc agttgtttct acctctaaag
     8941 gtgttatgac tgatcgtgca gcgcgccagg ctggtcttgg tggcgaaatt atctgctacg
     9001 tagcctaatc ggaggaaaaa atgtctcgtg ttgctaaagc accggtcgtt gttcctgccg
     9061 gcgttgacgt aaaaatcaac ggtcaggtta ttacgatcaa aggtaaaaac ggcgagctga
     9121 ctcgtactct caacgatgct gttgaagtta aacatgcaga taataccctg accttcggtc
     9181 cgcgtgatgg ttacgcagac ggttgggcac aggctggtac cgcgcgtgcc ctgctgaact
     9241 caatggttat cggtgttacc gaaggcttca ctaagaagct gcagctggtt ggtgtaggtt
     9301 accgtgcagc ggttaaaggc aatgtgatta acctgtctct gggtttctct catcctgttg
     9361 accatcagct gcctgcgggt atcactgctg aatgtccgac tcagactgaa atcgtgctga
     9421 aaggcgctga taagcaggtg atcggccagg ttgcagcgga tctgcgcgcc taccgtcgtc
     9481 ctgagcctta taaaggcaag ggtgttcgtt acgccgacga agtcgtgcgt accaaagagg
     9541 ctaagaagaa gtaaggtaac actatggata agaaatctgc tcgtatccgt cgtgcgaccc
     9601 gcgcacgccg caagctccag gagctgggcg caactcgcct ggtggtacat cgtaccccgc
     9661 gtcacattta cgcacaggta attgcaccga acggttctga agttctggta gctgcttcta
     9721 ctgtagaaaa agctatcgct gaacaactga agtacaccgg taacaaagac gcggctgcag
     9781 ctgtgggtaa agctgtcgct gaacgcgctc tggaaaaagg catcaaagat gtatcctttg
     9841 accgttccgg gttccaatat catggtcgtg tccaggcact ggcagatgct gcccgtgaag
     9901 ctggccttca gttctaaggt agaggtgtaa gatggctcac atcgaaaaac aagctggcga
     9961 actgcaggaa aagctgatcg cggtaaaccg cgtatctaaa accgttaaag gtggtcgtat
    10021 tttctccttc acagctctga ctgtagttgg cgatggtaac ggtcgcgttg gttttggtta
    10081 cggtaaagcg cgtgaagttc cagcagcgat ccagaaagcg atggaaaaag cccgtcgcaa
    10141 tatgattaac gtcgcgctga ataacggcac tctgcaacac cctgttaaag gtgttcacac
    10201 gggttctcgc gtattcatgc agccggcttc cgaaggtacc ggtatcatcg ccggtggtgc
    10261 aatgcgcgcc gttctggaag tcgctggggt tcataacgtt ctggctaaag catatggttc
    10321 caccaacccg atcaacgtgg ttcgtgcaac tattgatggc ctggaaaata tgaattctcc
    10381 agaaatggtc gctgccaagc gtggtaaatc cgttgaagaa attctgggga aataaaccat
    10441 ggcaaagact attaaaatta ctcaaacccg cagtgcaatc ggtcgtctgc cgaaacacaa
    10501 ggcaacgctg cttggcctgg gtctgcgtcg tattggtcac accgtagagc gcgaggatac
    10561 tcctgctatt cgcggtatga tcaacgcggt ttccttcatg gttaaagttg aggagtaaga
    10621 gatgcgttta aatactctgt ctccggccga aggctccaaa aaggcgggta aacgcctggg
    10681 tcgtggtatc ggttctggcc tcggtaaaac cggtggtcgt ggtcacaaag gtcagaaatc
    10741 tcgttctggc ggtggcgtac gtcgcggttt cgagggtggt cagatgcctc tgtaccgtcg
    10801 tctgccgaaa ttcggcttca cttctcgtaa agcagcgatt acagccgaag ttcgtctgtc
    10861 tgacctggct aaagtagaag gcggtgttgt agacctgaac acgctgaaag cggctaacat
    10921 tatcggtatc cagatcgagt tcgcgaaagt gatcctggct ggcgaagtaa cgactccggt
    10981 aactgttcgt ggcctgcgtg ttactaaagg cgctcgtgct gctatcgaag ctgctggcgg
    11041 taaaatcgag gaataagtag cagatggcta aacaaccggg attagatttt caaagtgcca
    11101 aaggtggctt aggcgagctg aaacgcagac tgctgtttgt tatcggtgcg ctgattgtgt
    11161 tccgtattgg ctcttttatt ccgatccctg gtattgatgc cgctgtactt gccaaactgc
    11221 ttgagcaaca gcgaggcacc atcattgaga tgtttaacat gttctctggt ggtgctctca
    11281 gccgtgcttc tatctttgct ctggggatca tgccgtatat ttcggcgtcg atcattatcc
    11341 agctgctgac ggtggttcac ccaacgttgg cagaaattaa gaaagaaggg gagtctggtc
    11401 gtcgtaagat cagccagtac acccgttacg gtactctggt gctggcaata ttccagtcga
    11461 tcggtattgc taccggtctg ccgaatatgc ctggtatgca aggcctggtg attaacccag
    11521 gctttgcatt ctacttcacc gctgttgtaa gtctggtcac aggaacgatg ttcctgatgt
    11581 ggttgggcga acagattact gaacgaggta tcggcaacgg tatttcaatc attatcttcg
    11641 ccggtattgt cgcgggactc ccgccagcca ttgcccatac tatcgagcaa gcgcgtcaag
    11701 gcgacctgca cttcctcgtg ttgctgttgg ttgcagtatt agtatttgca gtgacgttct
    11761 ttgttgtatt tgttgagcgt ggtcaacgcc gcattgtggt aaactacgcg aaacgtcagc
    11821 aaggtcgtcg tgtctatgct gcacagagca cacatttacc gctgaaagtg aatatggcgg
    11881 gggtaatccc ggcaatcttc gcttccagta ttattctgtt cccggcgacc atcgcgtcat
    11941 ggttcggggg cggtactggt tggaactggc tgacaacaat ttcgctgtat ttgcagcctg
    12001 ggcaaccgct ttatgtgtta ctctatgcgt ctgcaatcat cttcttctgt ttcttctaca
    12061 cggcgttggt tttcaacccg cgtgaaacag cagataacct gaagaagtcc ggtgcatttg
    12121 taccaggaat tcgtccggga gagcaaacgg cgaagtatat cgataaagta atgacccgcc
    12181 tgaccctggt tggtgcgctg tatattacct ttatctgcct gatcccggag ttcatgcgtg
    12241 atgcaatgaa agtaccgttc tacttcggtg ggacctcact gcttatcgtt gttgtcgtga
    12301 ttatggactt tatggctcaa gtgcaaactc tgatgatgtc cagtcagtat gagtctgcat
    12361 tgaagaaggc gaacctgaaa ggctacggcc gataattggt cgcccgagaa gttacggaga
    12421 gtaaaaatga aagttcgtgc ttccgtcaag aaattatgcc gtaactgcaa aatcgttaag
    12481 cgtgatggtg tcatccgtgt gatttgcagt gccgagccga agcataaaca gcgccaaggc
    12541 tgattttttc gcatattttt cttgcaaagt tgggttgagc tggctagatt agccagccaa
    12601 tcttttgtat gtctgtgcgt ttccatttga gtatcctgaa aacgggcttt tcagcatgga
    12661 acgtacatat taaatagtag gagtgcatag tggcccgtat agcaggcatt aacattcctg
    12721 atcataagca tgccgtaatc gcattaactt cgatttatgg cgtcggcaag acccgttcta
    12781 aagccatcct ggctgcagcg ggtatcgctg aagatgttaa gatcagtgag ctgtctgaag
    12841 gacaaatcga cacgctgcgt gacgaagttg ccaaatttgt cgttgaaggt gatctgcgcc
    12901 gtgaaatcag catgagcatc aagcgcctga tggatcttgg ttgctatcgc ggtttgcgtc
    12961 atcgtcgtgg tctcccggtt cgcggtcagc gtaccaagac caacgcacgt acccgtaagg
    13021 gtccgcgcaa accgatcaag aaataatcgg ggtgattgaa taatggcaaa ggcaccaatt
    13081 cgtgcacgta aacgtgtaag aaaacaagtc tctgacggcg tggctcatat ccatgcttct
    13141 ttcaacaaca ccatcgtgac tatcactgat cgtcagggta acgcgttggg ttgggcaaca
    13201 gccggtggtt ccggtttccg tggttctcgc aaatccactc cgtttgcagc tcaggttgca
    13261 gcagagcgtt gcgctgacgc cgtgaaagaa tacggcatca agaatctgga agttatggtt
    13321 aaaggtccgg gtccaggccg cgaatctact attcgtgctc tgaacgccgc aggtttccgc
    13381 atcactaata ttactgatgt gactccgatc cctcataacg gttgtcgtcc gccgaaaaaa
    13441 cgtcgcgtat aacgcctcgt tttccaggtt tgttggagaa agaaaatggc aagatatttg
    13501 ggtcctaagc tcaagctgag ccgtcgtgag ggcaccgact tattccttaa gtctggcgtt
    13561 cgcgcgatcg ataccaagtg taaaattgaa caagctcctg gccagcacgg tgcgcgtaaa
    13621 ccgcgtctgt ctgactatgg tgtgcagttg cgtgaaaagc aaaaagttcg ccgtatctat
    13681 ggtgtgctgg agcgtcagtt ccgtaactac tacaaagaag cagcacgtct gaaaggcaac
    13741 accggtgaaa acctgttggc tctgctggaa ggtcgtctgg acaacgttgt ataccgtatg
    13801 ggcttcggtg ccactcgtgc agaagcacgt cagctggtta gccataaagc aattatggta
    13861 aacggtcgtg ttgttaacat cgcttcttat caggttagtc cgaatgacgt tgtaagcatt
    13921 cgtgagaaag cgaagaagca gtctcgcgtg aaagccgctc tggagctggc tgagcagcgt
    13981 gaaaagccaa cctggctgga agttgatgct ggcaagatgg aaggtacgtt taagcgtaag
    14041 ccggagcgtt ctgatctgtc tgcggacatt aacgaacacc tgatcgtcga gctttactcc
    14101 aagtaaagct tagtaccaaa gagaggacac aatgcagggt tctgtgacag agtttctaaa
    14161 accgcgcctg gttgatatcg agcaagtgag ttcgacgcac gccaaggtga cccttgagcc
    14221 tttagagcgt ggctttggcc atactctggg taacgcactg cgccgtattc tgctctcatc
    14281 gatgccgggt tgcgcggtga ccgaggttga gattgatggt gtactacatg agtacagcac
    14341 caaagaaggc gttcaggaag atatcctgga aatcctgctc aacctgaaag ggctggcggt
    14401 gagagttcag ggcaaagatg aagttattct taccttgaat aaatctggca ttggccctgt
    14461 gactgcagcc gatatcaccc acgacggtga tgtcgaaatc gtcaagccgc agcacgtgat
    14521 ctgccacctg accgatgaga acgcgtctat tagcatgcgt atcaaagttc agcgcggtcg
    14581 tggttatgtg ccggcttcta cccgaattca ttcggaagaa gatgagcgcc caatcggccg
    14641 tctgctggtc gacgcatgct acagccctgt ggagcgtatt gcctacaatg ttgaagcagc
    14701 gcgtgtagaa cagcgtaccg acctggacaa gctggtcatc gaaatggaaa ccaacggcac
    14761 aatcgatcct gaagaggcga ttcgtcgtgc ggcaaccatt ctggctgaac aactggaagc
    14821 tttcgttgac ttacgtgatg tacgtcagcc tgaagtgaaa gaagagaaac cagagttcga
    14881 tccgatcctg ctgcgccctg ttgacgatct ggaattgact gtccgctctg ctaactgcct
    14941 taaagcagaa gctatccact atatcggtga tctggtacag cgtaccgagg ttgagctcct
    15001 taaaacgcct aaccttggta aaaaatctct tactgagatt aaagacgtgc tggcttcccg
    15061 tggactgtct ctgggcatgc gcctggaaaa ctggccaccg gcaagcatcg ctgacgagta
    15121 accggatcac aggttaaggt tttactgaga aggataaggt catgcgccat cgtaagagtg
    15181 gtcgtcaact gaaccgcaac agcagccatc gccaggctat gttccgcaat atggcaggtt
    15241 cactggttcg tcatgaaatc atcaagacga ctctgcctaa agcgaaagag ctgcgccgcg
    15301 tagttgagcc gctgattact cttgccaaga ctgatagcgt tgctaatcgt cgtctggcat
    15361 tcgcccgtac tcgtgataac gagatcgtgg caaaactgtt taacgaactg ggcccgcgtt
    15421 tcgcgagccg tgccggtggt tacactcgta ttctgaagtg tggcttccgt gcaggcgaca
    15481 acgcgccgat ggcttacatc gagctggttg atcgttcaga gaaagcagaa gctgctgcag
    15541 agtaatctga agcaacgtaa aaaaacccgc cccggcgggt ttttttatac ccgtagcatc
    15601 cccacttatc tacaatagct gtactctttt tgttcatccc ctggagtatt tatgtggtta
    15661 cttgaccagt gggcagagcg tcatatagca gaagcgcaag cgaaaggtga gtttgataac
    15721 ctggcaggta gcggcgaacc attgatactg gatgatgatt ctcacgtgcc accggaatta
    15781 cgtgcggggt atcgcttgct gaagaatgcc ggttgcttac cgccagaact tgagcaacgg
    15841 agagaagcaa ttcagcttct ggatatcctg agaggtatcc gtcacgatga tccgcaatat
    15901 caagaggtta gccgtcgatt gtcattactg gaattgaagc tgcgacaagc tggattgagt
    15961 accgattttt tacgcggcga ttatgctgac aagttgttgg acaaaatcaa cgataactag
    16021 tggagtatgt atgtatcgca ttggtgagct ggcaaaaatg gcggaagtaa cacctgacac
    16081 gattcgttat tacgaaaaac agcagatgat ggagcatgaa gtgcgtactg aaggcgggtt
    16141 tcgcctgtat accgaaagcg atctccagcg attgaaattt atccgccatg ccagacaact
    16201 aggtttcagt ctggagtcga tccgcgagtt gctgtcgatc cgcatcgatc ctgaacacca
    16261 tacctgtcag gagtcaaaag gcaatgtgca ggaaagattg caggaagtcg aagcacggat
    16321 agccgagttg cagagtatgc agcgttcctt gcaacgcctt aacgatgcct gttgtgggac
    16381 cgcccatagc agtgtttatt gttcgattct tgaagctctt gaacaagggg cgagtggcgt
    16441 taagagtggc tgttgatttt ttgcactggc aggattacac tcgcgccgta aaataaccaa
    16501 ttggagtttt tatgagccga tatcagcata ctaaagggca gataaaggat aatgcgatag
    16561 aagcattact acatgatccc ttattccgac agcgcgtaga gaaaaataag aaggggaaag
    16621 gcagttacat gcgaaaagga aaacatggca atcgggggaa ctgggaggcc agtggcaaga
    16681 aagtaaatca cttttttacc actggccttc tgctttcagg cgtttgttaa gagcggttat
    16741 tctgctcttt cagcaaatca cgaatttctg tcagtaatac ttcttcttta gttggtgctg
    16801 gtgcggctgc tggttcttct tttttccgat tcagtttgtt gattagctta atcgccataa
    16861 agatggcaaa ggccacaatc agaaaatcaa agacgttttg aatgaagaca ccgtaatgca
    16921 tcacaacagc agggatatcc ccctgcgcat cgcgtagcgt gacagcaaac tgtttaaagt
    16981 cgatcccgcc aattaataag cccagaggtg gcatgatgat atcggcaacc agtgaagata
    17041 caatcttccc gaatgccgca ccgataatga cacccaccgc caaatccacc acgttcccgc
    17101 gcatcgcaaa ttcgcgaaat tctttaataa tgctcatgtt attctcccta tgaagccgac
    17161 aaccataagt ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct
    17221 tgataatcaa agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag
    17281 ggcttggctg gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa
    17341 ttacgtgatc gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac
    17401 gtaccaccgc tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga
    17461 caacgcgtga ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc
    17521 ggcgcaatga ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa
    17581 tagttgcttg ttgtggtgaa atcgcaatat cgatcacgct cccctgaacc agatccacat
    17641 aagcgcgacg ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg
    17701 cggacatgat attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga
    17761 tatgttcttc ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct
    17821 gcaacttttc cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt
    17881 aatctttttc cagacgacgc gccagccctg caccgatatt accgccacca accagcatga
    17941 tccgcttata cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg
    18001 aagcggcaat aaagaacact tcatcaccag cttccacaat ggtcgaacct tgtggacgaa
    18061 tggggcgatc gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc
    18121 gcatggtcga aagtgcgtta ccaatcagcg ggccgccata ataggcttta accacagcga
    18181 ggctgacttt accctcagcg aagttcacca cctgcaatgc gccgggatac tcaatcagtc
    18241 ggtaaatatt atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat
    18301 ctgaatgaaa tagcttatcg gcatcgcgca cgtagtctgg tgagcggatg cgagcgatgc
    18361 gattaggggt gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg
    18421 aactggttac agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg
    18481 gatgagagcc atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac
    18541 gctcaccgtt ggtatcgaca acagtgatat cgttgttctc gccaaccagg ttttccgcca
    18601 gtgtgccgcc aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat
    18661 ctcatcactt tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggca
    18721 gattttgttt acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac
    18781 gttgcaaaaa ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat
    18841 agaccagggt tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca
    18901 attgcgcgag ttccgggata tcgcgatcgc gacgtaacca tttaatatcc ggatggcgac
    18961 gaatcacacc ggttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc
    19021 cacaccattg ggaagggtag cggccatcac cttgtttcac ggtcgctttc ataccaaggc
    19081 gttttaaatt gtcgtaaacg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct
    19141 gcgcttctgg tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat
    19201 ccagaatgtg ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat
    19261 cctgaacggt aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt
    19321 ccagccgtac agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca
    19381 acaatgcaag ccagctgtcg cgggaatgat gcgtacgatt aacgcgcagc cacattggcg
    19441 gacgctggtt attggcttcg acgatggatt gccactgctc tggatacgct ttttgcagac
    19501 gcttcagcaa ccaggaagga tgcagataac gtgcatcact ggcattaaac tcggctaata
    19561 actcttcttg ctgacgctgg aactggcgta atacaccgtt tatcaaccct ttaagttgcg
    19621 gacgcttaat tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc
    19681 gggtataaag cagttgatac aaaccaacca taatcaggta atgcacggtc cgctgtttgc
    19741 cggtcatcgg acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca
    19801 gtacgccaaa gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga
    19861 gcggtggcag aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg
    19921 ccgccatgct acgtaaatta cgttgttttt tcataagtat aaaaacgccc ggcaagaccg
    19981 ggcttagaag agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag
    20041 agttcaggag gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt
    20101 tcaggatgcc atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc
    20161 ctggtgcagc gttggttgcc gtatcaatga ccgatgcttt ccagacttta accggctgtc
    20221 cttcaatttc cagccagctc attggccatg gattgaaagc gcgaatgcag cgttcaagct
    20281 gtgctgccga aagtgaccag tcaatacgcg cttcttcttt actcaacttt tcggcgtaag
    20341 tgacaagagt ttcgtcctga acttctggtt tcgccgtgcc gtctgccagt tgtttcaacg
    20401 tggtgataag cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat
    20461 cttctgcagt aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccgacat
    20521 ccatttgcat aatggtcaca ccagtttctg catcacccgc ccatagtgag cgttggattg
    20581 gtgcagcacc gcgccagcgt ggcagcagtg aaccatgaac gttgatacag ccaagacgcg
    20641 gcatctccag cactgcttta ggcagaatta agccataggc gacgacgacc ataacatcag
    20701 cctgcagatc ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa
    20761 cgggcagacc tttttcctca gccagaactt taaccgggct gggcatcagt tttttaccgc
    20821 gtcctgccgg tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca
    20881 gcgcgtcgag atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt
    20941 ctgacacgtt agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt
    21001 ctgacgaata cgttgttgtt tcaacggtga cagataatcc ataaacagtt tgccgaccag
    21061 gtgatccatc tcatgctgaa tacagatggc taacagaccg tctgcttcca attcaaatgg
    21121 tttaccgtcg cggtccaggg cgcgaatttt aactttctct gcgcgtggca ctaaagcacg
    21181 ttgttcaggg atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag
    21241 ctccggattg attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat
    21301 acgttgatgg atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt
    21361 ctcgaacata tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc
    21421 aactttgcga agccgctcgt ccggaat
//
LOCUS       DAEVZA010000088        21272 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEVZA010000088 DAEVZA010000000
VERSION     DAEVZA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.088"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            105..1118
                     /gene="oppD"
                     /locus_tag="LAF92_003267"
     CDS             105..1118
                     /gene="oppD"
                     /locus_tag="LAF92_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBI9792224.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            1115..2119
                     /gene="oppF"
                     /locus_tag="LAF92_003268"
     CDS             1115..2119
                     /gene="oppF"
                     /locus_tag="LAF92_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBI9792225.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(2172..2501)
                     /locus_tag="LAF92_003269"
     CDS             complement(2172..2501)
                     /locus_tag="LAF92_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBI9792226.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(2536..3996)
                     /gene="cls"
                     /locus_tag="LAF92_003270"
     CDS             complement(2536..3996)
                     /gene="cls"
                     /locus_tag="LAF92_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBI9792227.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            4139..4312
                     /locus_tag="LAF92_003271"
     CDS             4139..4312
                     /locus_tag="LAF92_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBI9792228.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(4367..5620)
                     /gene="kch"
                     /locus_tag="LAF92_003272"
     CDS             complement(4367..5620)
                     /gene="kch"
                     /locus_tag="LAF92_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBI9792229.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(5920..6216)
                     /locus_tag="LAF92_003273"
     CDS             complement(5920..6216)
                     /locus_tag="LAF92_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBI9792230.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            6440..7159
                     /gene="tonB"
                     /locus_tag="LAF92_003274"
     CDS             6440..7159
                     /gene="tonB"
                     /locus_tag="LAF92_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBI9792231.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(7199..7597)
                     /gene="yciA"
                     /locus_tag="LAF92_003275"
     CDS             complement(7199..7597)
                     /gene="yciA"
                     /locus_tag="LAF92_003275"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBI9792232.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(7702..8241)
                     /locus_tag="LAF92_003276"
     CDS             complement(7702..8241)
                     /locus_tag="LAF92_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBI9792233.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(8271..9014)
                     /locus_tag="LAF92_003277"
     CDS             complement(8271..9014)
                     /locus_tag="LAF92_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBI9792234.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(9042..9131)
                     /locus_tag="LAF92_003278"
                     /pseudo
     CDS             complement(9042..9131)
                     /locus_tag="LAF92_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            9371..10009
                     /gene="ompW"
                     /locus_tag="LAF92_003279"
     CDS             9371..10009
                     /gene="ompW"
                     /locus_tag="LAF92_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBI9792235.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(10069..10575)
                     /locus_tag="LAF92_003280"
     CDS             complement(10069..10575)
                     /locus_tag="LAF92_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBI9792236.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(10621..11121)
                     /locus_tag="LAF92_003281"
     CDS             complement(10621..11121)
                     /locus_tag="LAF92_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBI9792237.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(11207..11386)
                     /locus_tag="LAF92_003282"
     CDS             complement(11207..11386)
                     /locus_tag="LAF92_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBI9792238.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(11767..12573)
                     /gene="trpA"
                     /locus_tag="LAF92_003283"
     CDS             complement(11767..12573)
                     /gene="trpA"
                     /locus_tag="LAF92_003283"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBI9792239.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(12573..13766)
                     /gene="trpB"
                     /locus_tag="LAF92_003284"
     CDS             complement(12573..13766)
                     /gene="trpB"
                     /locus_tag="LAF92_003284"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBI9792240.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(13778..15139)
                     /gene="trpCF"
                     /locus_tag="LAF92_003285"
     CDS             complement(13778..15139)
                     /gene="trpCF"
                     /locus_tag="LAF92_003285"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBI9792241.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(15140..16735)
                     /gene="trpD"
                     /locus_tag="LAF92_003286"
     CDS             complement(15140..16735)
                     /gene="trpD"
                     /locus_tag="LAF92_003286"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBI9792242.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(16735..18297)
                     /gene="trpE"
                     /locus_tag="LAF92_003287"
     CDS             complement(16735..18297)
                     /gene="trpE"
                     /locus_tag="LAF92_003287"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBI9792243.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(18319..18412)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(18389..18433)
                     /gene="trpL"
                     /locus_tag="LAF92_003288"
     CDS             complement(18389..18433)
                     /gene="trpL"
                     /locus_tag="LAF92_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBI9792244.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            18571..19452
                     /gene="yciV"
                     /locus_tag="LAF92_003289"
     CDS             18571..19452
                     /gene="yciV"
                     /locus_tag="LAF92_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBI9792245.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            19449..20069
                     /locus_tag="LAF92_003290"
     CDS             19449..20069
                     /locus_tag="LAF92_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBI9792246.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIYGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            20170..21042
                     /gene="rluB"
                     /locus_tag="LAF92_003291"
     CDS             20170..21042
                     /gene="rluB"
                     /locus_tag="LAF92_003291"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBI9792247.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNS"
     gene            complement(21082..>21272)
                     /locus_tag="LAF92_003292"
     CDS             complement(21082..>21272)
                     /locus_tag="LAF92_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBI9792248.1"
                     /translation="YDYLPLEEVVQALNERPHQQTVIITGRGCHRDILELADTVSELR
                     PIKHAFDAGVKAQIGIDY"
BASE COUNT         5394 a         5297 c         5356 g         5225 t
ORIGIN      
        1 ttgttcccag cgggattcct cgtggtgacg ctgttttgtt tcaactttat cggcgatggc
       61 ttgcgtgatg ccctcgaccc gaaagatcgt taaggagtgc agccatgagc gtaattgaaa
      121 ctgcaactgt gccgctcgca caacaacagg ctgacgcact gctgaacgtg aaagatttgc
      181 gtgtcacctt tagtaccccg gacggcgacg tcacggcggt caatgatttg aatttttccc
      241 tacgtgccgg agaaacgctg ggtattgtag gtgagtctgg ttcgggtaaa tcgcaaactg
      301 catttgcgtt gatgggcctg ttggctgcca acggacgtat tggcggatcg gcaaccttca
      361 atgggcgtga aatcctcaat ttgccagagc atgaactcaa taaactgcgc gctgaacaaa
      421 tctcaatgat ttttcaggac ccaatgactt cgttgaatcc ctatatgcgc gtcggtgagc
      481 agttaatgga agtgctgatg ctgcataaga acatgagcaa agctgaggcg tttgaagagt
      541 cggtgcggat gctcgatgcg gtaaaaatgc cggaagcgcg taaacgcatg aaaatgtacc
      601 cgcacgaatt ttctggcggc atgcgtcagc gagtcatgat tgcgatggca ttgctatgtc
      661 gacctaagct actgattgcg gatgaaccca ctactgcgct ggacgtcacc gtacaggcgc
      721 agatcatgac gctattaaat gaactgaagc gggaatttaa taccgccatc attatgatca
      781 cccacgatct tggcgtggtg gcggggatct gtgacaaagt gctggtaatg tacgccgggc
      841 gcacgatgga atatggcaat gcgcgcgatg tcttttatca acccgttcat ccttattcta
      901 tcggtttgct caacgcggtg ccgcgtcttg atgcggaagg tgaaacaatg ttgaccatcc
      961 cgggtaatcc gccaaacttg ctgcgattac cgaaaggttg cccgttccag ccacgttgtc
     1021 cacatgcgat ggaaatttgt agtagcgcac cgccgctgga agagtttacg cctggccgtc
     1081 tgcgtgcttg ctttaaaccg gtggaggaac tgttatgaat gctgtaactg aaggaagaaa
     1141 agtcctcctt gaaatcgccg atcttaaagt gcactttgaa atcaaagatg gcaaacagtg
     1201 gttctggcaa ccgccgaaaa cgctcaaagc cgtcgatggt gtcactcttc gcctgtatga
     1261 aggggaaaca ttaggtgtgg taggggaatc gggatgcggt aagtccacct ttgctcgcgc
     1321 catcatcggt ttggtcaagg cgaccgacgg tcatgttgcc tggttaggta aagagttgct
     1381 gggcatgaag cccgatgaat ggcgtgccgt tcgcagtgat attcagatga ttttccagga
     1441 tccgttggca tcgctaaacc cgcgtatgac catcggcgag atcatcgctg aaccactgcg
     1501 tacttatcat ccgaaaatgt cacgccagga agttcgcgag cgcgtgaagg cgatgatgct
     1561 gaaagtcggg ttattgccta acctgattaa ccgctatccg catgagttct ccggtgggca
     1621 gtgccagcgt atcgggattg ctcgtgctct tattcttgaa ccgaagctga ttatctgcga
     1681 tgagccggtt tcggcgctgg acgtgtcaat tcaggcgcag gtggtcaacc tgctccagca
     1741 gctgcaacgt gagatgggat tgtcattaat ttttatcgct catgacctgg ccgtggtaaa
     1801 acacatttcc gatcgtgtgt tggtgatgta tctcggccat gcggtagaac tggggaccta
     1861 tgatgaggtc taccacaatc cactacatcc ttacaccagg gcattgatgt cggcagtccc
     1921 catacctgat ccggatctgg agaagaacaa aaccatccag ttactggaag gggaattacc
     1981 gtcgccgatc aacccgcctt ccggttgtgt tttccgtacc cgttgcccga ttgccggtcc
     2041 ggagtgcgcc aaaacacgtc ctgttctgga ggggagtttc agacacgccg tttcttgcct
     2101 gaaagtcgat ccgctttaaa cacaataagg gctgacagtt gtcagccctt attcacgcta
     2161 aaagcgatta tttattcccg ccagatgata tggcaaagtt tgtgatcttt ttcgcggcat
     2221 aacagaatgc gggcgaaaac atcgttgatt tcaccatctt cactgtccgc cagaccaatc
     2281 accacttcgg caaaaaagtc tgggttcaaa tcgaaatcaa catgttcctg ccagtcttcc
     2341 gccggatcga ataactccgc gccgccgcgc tcttcaaact gaagattgaa cagcagaaca
     2401 tcagctggat cgagattgtc ggcagccagt tcgagaaaaa tatcgtaggc ctgctcaagc
     2461 gtttcatctt cagtcaggcg attgtttaga tccatatcca tagtcactac ctgtttaacc
     2521 tctgttggcg acgttttaca gcaacgggct gaagaagtaa aacagtcgct cggcgacacg
     2581 ttgccataat ggacgtttta gccataaacg ggcatcgagc agacgtgaac gcgaaatata
     2641 atcgtcctga acggcggcaa ggtctgcacc aaaaccttta tcgtcgattg ccagggtaat
     2701 ctcgaaattc agccacagac tacgcatatc aaggttaacg gtgccaacca gacttagttc
     2761 gccatcgacc agtacgctct tggtatgcag taacccgcct tcaaactgat aaattttaac
     2821 cccagcagcc aacagctccg taaagaatgc gcgactggcc cagccgacca gcatcgagtc
     2881 attttttcgc ggaaggataa tactgacatc caccccgcgc tgcgccgccg tgcaaatcgc
     2941 atgaagtaaa tcatcgcttg gcacaaagta gggcgttgtc atgatcaaat attcacgcgc
     3001 cgaataagcc gcagtcaata atgcctggtg aatgagatct tccggaaagc cggggccaga
     3061 agcaattgtg tgaatggtgt gaccgctggc ctgttcaaac ggcataatat tgacatctgg
     3121 tggtggcggc agaatacgtt ttccggtttc aatctcccag tcgcaggaat aaataatccc
     3181 catcgcggtg gcgatggggc cttccatacg cgccatcaga tcaatccatt gccctacgcc
     3241 cgcatcttgt ttgaagtagc gaggatcgac catattcatg ctgccggtgt acgcgatgta
     3301 attatcgatc atgatcatct tgcgatgttg gcgcaggtcc atacggcgta aaaacacacg
     3361 catcagattg acctttaagg cttcgaccac ttcaataccg gcattacgca ttagctcggg
     3421 ccacgggctg cggaaaaaag ccacactccc ggcggagtcg agcatcaatc ggcaatgaat
     3481 gccgcgtcgc gcagcggcca ttaatgattc agccacctgg tccgccatgc cgccgggctg
     3541 ccagatataa aacaccatct caatattatg gcgcgcgagc tggatgtcgc ggattaacgc
     3601 ctgcatcaca tcatctgact cggtcatcag ttgtagctga ttccctttga ccccagcgat
     3661 cccctgacga cgctcgcaaa gcttgaataa tggcgcagcg acactgctat tttcttcggc
     3721 gaagatatgc ttacaggctt taaggtcgtt aagccatttt gcggtggaag gccacatcgc
     3781 tctggcgcgc tcagcgcggc gtttgcctaa atggagctcg ccaacggcaa gataggcaat
     3841 aattccgact aacggcagaa tgtaaataat caacagccag gccatcgcgg agggaactgc
     3901 gcgtcgtttc attagaatgc gtaaagttac gcctgcaatg agcaaccagt atcccagaat
     3961 ggccaaccaa ctcaccaacg tataaacggt tgtcatagat tagaaatcct tttgaaagcg
     4021 cattgttatg agtttacgca tatggattca tctggcaaat aaaaacgcgg gaaaagcact
     4081 ggtcagcgtc gggggtgggg gtataatgac cattctgtta ttgcatagag tagttaacat
     4141 gaagcggagt agaacggaag tggggcgctg gcgcatgcag cgtcaggcta gccgacgtaa
     4201 atcgcgttgg cttgaggggc aatcgcgccg aaatatgcgt atccacagca tcaggaagtg
     4261 cattctaaac aaacagcgta actcgttatt gtttgcgatc tacaatatct aaatgtactg
     4321 ggcaccattg cggtgcccaa taagtttact cttccgtctt taaactctat ttttgcgccg
     4381 attctttact gtcagctttc gtttcttgtt catcgttatc gctgaaaata ccatgacctg
     4441 agttcaacaa cattgaaacg agcatatcat tattaatctc ttcaccattt aaaactcgcg
     4501 ccagaatttc gctgccaaac agttgcggtg agagaatgat atccggatgt accatcttaa
     4561 tcttattcag gtttttgcta tcactgacgg cgagaactgt tttgacatca ctgctcatat
     4621 cttttgccga gagtacaaca aacgcgttat ctgcatcgtt atcactcagc gccagaatgg
     4681 cccggcaacg atcgattccc gctttcttta atactgaact gtcattactg tcaccgggga
     4741 taacatcagc gttatcgcct aagcgttgct caagttgctt gatatcatct tcaggcaagt
     4801 tgctgataac cgttacgttt tgtccgcgtt gattcagttg caggatcgta ttgatggcga
     4861 gaatcgaatg tccgcaaaca ataaaatgat ctttacgatg cattgtatga ttgtttcctt
     4921 ttacaagttt gttgaatccc ccgcggataa gcggaccaaa aattgaggtc atagatgtgg
     4981 caaaaacggt aatgccggaa ataatgaccg aaatagtgaa caatcgtgct gattcagaaa
     5041 cagggacaat atcgccatag ccgacggttg acatggtttc tatcgaaaaa tagaacgcgg
     5101 tcatcaaact ttctattcgc ggattaaaac cttcgcttaa ataaagcgca ccgtaggttg
     5161 agtaaaacag taatgtcgtg aaactaataa atgcaaaaat tgtcccggct gcggcgctac
     5221 tgtgggagaa gtctttgcgc agtatcagca aaaacaccag cgtaaaaatg caaaatccaa
     5281 tactaaattt cagccaggga taaaaatgca gggtgtaaat tagcgctatc aacaacaaaa
     5341 tgatactgat tgcccatgcg agctttgccc ggaacagtaa gccaatggag ttcaggacaa
     5401 gaaagacgcc gagcataaat agtggggcgt ttgccagcaa ggagagatcc aactctgaaa
     5461 atgctttgat atggaaaata tcaagcaaat tgaccgacat accatagatt gttttaaaaa
     5521 taagcaatcc atttaaaaag acggccagcg cgagaatgtc gtgccgaagg gtcacccata
     5581 aatttgttgc tgtttgtttg aatgtagccc agtgactcac tgatacctct gtaaccacaa
     5641 ttttgtttaa ttatcttagc gtgcacattt tctgaacata catgcagcgc gtgaatgtgt
     5701 taaacaatag tagatgactt tatccgcagt aatcaaaggt tttgagcgag caactgtacc
     5761 tcaggaaagg tcatttttcc tgaatatgtt cacatcatat aaagaaatac agataaagtt
     5821 attatctgct tgtggtggtg aatgcactga ccggctataa ggaaaggcca aacaagaaca
     5881 cggttgcaaa aaccgtgccc ttaaatattg aatctctatt cagaacactt tcttaaatgg
     5941 tttcactgaa acgtgttcat agacacctgc cgctacgtac gggtcagcat cggcccaggc
     6001 ctgagctgct tccagcgatt caaattcagc aataacggtt gagccagtaa atcccgcagc
     6061 ccctggatcg ttactgtcta ccgctggcat tggaccggct gtcaacaaac gaccttcatc
     6121 atgcagtaac tgtaaacgtg ctaaatgtgc cggacgaacg gaaaggcgtt tttcgaggct
     6181 atcagctttg tcttgagcgt aaataacata caacacgggc acaactcctt gttcgggaaa
     6241 gttgtaaagt acgttatttg aaagggcgaa gatctgcaac ggaaagatga tgtctttgtt
     6301 aagaccatgc ataaagtaag gtcaattacg ccaaaaatga cattttcact gatcctgatc
     6361 gtcttgcctt attgaatatg attgctattt gcatttaaaa tcgagacctg gtttttctac
     6421 tgaaatgatt atgacttcaa tgacccttga tttacctcgc cgcttcccct ggccgacgtt
     6481 actttcggtc tgcattcatg gtgctgttgt ggcgggtctg ctctatacct cggtacatca
     6541 ggttattgaa ctacctgcgc ctgcgcagcc gatttctgtc acgatggttg cgcctgctga
     6601 tctcgaaccg ccacaagccg ttcagccgcc accggagccg gtggtagagc cagaaccgga
     6661 acctgagccg atccccgaac cgccaaaaga agcaccggtg gtcattgaaa agccgaagcc
     6721 gaaacctaag ccaaaaccga agccggtgaa aaaggtacag gagcagccaa aacgcgatgt
     6781 caaacccgta gagtcgcgtc cggcatcacc gtttgaaaat acggcacccg cgcgccccac
     6841 atcaagtaca gcaacggctg caaccagcaa gccggttacc agtgtggctt caggaccacg
     6901 cgcattaagc cgtaatcagc cgcagtatcc ggcacgagca caggcattgc gcattgaagg
     6961 gcaggttaaa gttaaatttg acgtcacgcc ggatggtcgc gtggataacg tacaaatcct
     7021 ctcagccaag cctgcgaaca tgtttgagcg tgaggtgaaa aatgcgatgc gcagatggcg
     7081 ttatgagccg ggtaagccag gcagtgggat tgtggtgaat atcctgttta aaattaacgg
     7141 caccaccgaa attcagtaag cagaaagtca aaagcctccg accggaggct tttgattatt
     7201 actcaacagg taaggcgcga ggttttcctt caggatcaac cgcgacatac ttaaataatg
     7261 cttctgtcgc tttatagcgt tgcccgattg gttcagacgc tacttttttc acccacactt
     7321 caatattaat gctgaccgat gtcgtccctt tctggacaca gcgtgcatag cagcacacca
     7381 catcgccgac cgcaaccggc cgtaagaaag tcattccttc aacccgcacg gtcactacgc
     7441 gaccgtgggc aatttctttc gccagaatag cgccgccaat atccatttgt gacattaacc
     7501 aaccaccaaa gatgtcacca ttggcattgg tgtcggcggg catggctaaa gtacgtaaaa
     7561 caagatcgcc ctgagggacg ttatgtgttg tagacatggt aaaaccgact taaaaggaaa
     7621 atcacaggcg cgatgctact atgattttcc tgaggagaac agaggagata cagcgccagc
     7681 cccgaaggac tggcaatctg gttaggattt atcttcctgc ggcatgtggc ggtagatata
     7741 gataccgctt aacaatgtga agattaaggt aagggcggtc agtccaaaga ctttaaagtt
     7801 gacccaaata ttttgcggca gccagaatgc gatgtagatg tttgccagac cgcaaaggat
     7861 aaagaaaaca gcccaggcca gatttagctt cgaccatacc ggttgcggca gcgtgagttc
     7921 tttacccagc atccgctgaa ttagcggctt tttcatcacc cattggctga ctaacagggc
     7981 tcccgcaaac agagcataaa tgactgtaac cttccattta ataaactcat cattgtggaa
     8041 gaacagcgtc aagccaccga agacgaccac cagaacaaaa gtgatcaggg ccatcttctc
     8101 aaccttacga aagcgaaccc agctatagat aagcacaatc gccgtggcga cgatcagcgc
     8161 cgcagtagcc gcatagatgt cataaatctt gtaaaacgcg aaaaagacaa ccagcggtaa
     8221 aaaatcaaga aactgcttca ttttacgatt ccgtaatcaa atgcttaagg ttattggcga
     8281 atcaacatat acagacggaa cagatagatg agcaatacgg ctgaaatcag gttgctcaag
     8341 gtgttcgcca gtacggcacc aatttccggg gttaatgcgg caaaagaaga ggcaaaaagc
     8401 agcaacagtg tttttgccag caaccagctc agtactgcgg gtgccaccag acgcatattc
     8461 gcccaagtca gccgcatact gctacgcatc gaggcaaaaa tgcccatttt gtcctgtacc
     8521 agcatcaccg gtgccagggc cagtaaaatg gccatgataa ttcccggaac gaccactagc
     8581 atgatgccaa tctgtactaa aagggtagtc agaaaaatca gaataaataa ctttggcaat
     8641 atcggcgcac tggcaccaat agctcgcagc gcactgactc tctgacccgc agacaccagc
     8701 tggataatta atattacgcc tccggcgaga atggcgttac cgattaatcc tgaaaaagtg
     8761 gacgccgctg aagcctgcag caaaatttgt tgctgttccg gtgacatatt ctgaaccagg
     8821 tcgaacaacc cactactgcc gctaacgggc acgccgtcat tgagctgcgc aagctgtgca
     8881 tcactgggtg agaaaacatg ccctaacacc actgtgataa acgcacatag caacgatacc
     8941 aacagaatgg tcataaattg attacggaag aaatttccgg tgtcacggta tacggactgc
     9001 gccgtgatag acatgcactc tccttgagtt ttatgcaggt gttaattagc gggcaattgt
     9061 accctggtta agcctcgggt ggcagcatca ggtcttgtat ggaaaagcat atctttgtaa
     9121 agcggaggta attgctgacc ttcaggtgtg ggcaaaccgc ggcaagtggc gatcgagtta
     9181 atatttgcgt agcgaaaata tttaaaaatt gatttaaatc acgttaacca ggattctcaa
     9241 tgcaacttct aaattaaccc agatcaataa agggtgaatt atcatatgta atgtgatcta
     9301 tgtaggatca tttgttactc caatgtaggt atattcgtca cgtttttata accataacga
     9361 cggagcggat atgaaaaagt taacagtggc ggctttggca gtaacaactc ttctctctgg
     9421 cagtgccttt gcgcatgaag caggcgaatt ttttatgcgt gcaggttctg caaccgtacg
     9481 tccaacagaa ggtgctggtg gtacgttagg aagtctgggt ggattcagcg tgaccaataa
     9541 cactcaactg ggcctgacgt ttacttatat ggcgaccgac aacattggtg tggaattact
     9601 ggcagcgacg ccgttccgcc ataaaatcgg cacccgggcg accggcgata ttgcaaccgt
     9661 tcatcatctg ccgccaacac tgatggcgca gtggtatttt ggtgatgcca gcagcaaatt
     9721 ccgtccttat gttggggcag gtattaacta caccaccttc tttgataatg gatttaacga
     9781 tcatggcaaa gaggcggggc tttccgatct cagtctgaaa gattcctggg gagctgccgg
     9841 gcaggtgggg gttgattatc tgattaaccg tgactggttg gttaacatgt cagtctggta
     9901 catggatatc gataccaccg ccaattataa gctgggcggt gcacagcaac acgatagcgt
     9961 acgcctcgat ccgtgggtgt ttatgttctc agcaggatat cgtttttaat tccgcacaaa
    10021 aacgaccccg taatatacgg ggtcaataag gacatggtat aaagggtatt atttcttcgc
    10081 ttctacgcca tcagtttcag aacgaattaa aaatttctca gttgtttgcg gaatatgctg
    10141 aatcagccag tcggccattt gcttttcctc atttaaaatc gcttcgatga tcggaattga
    10201 ggctgtatca ccggcatttt ttgctgctgc taatagtgag gtataacagg ctatttcaaa
    10261 ttgctcgaag acatatccgc taatagagcc tttaactatt tcatcagaag ggaatatacc
    10321 accgattgac tgcccaagcg cagccatttt actcatggaa tctttaatga ctgaacgtga
    10381 aatgtcatta cgatcaagaa tagtttccag ttgaacaatc tggtttttgg tttcactgag
    10441 atgttgttca atacgagcgc gtagttcagg ataattatct atacgactag ccatggattc
    10501 aagcatagat tcggcttgct tttccattgc gtgggcgtca cgtaaccagt catgataatg
    10561 ttcaatacga ttcatgaaaa tgtcctcttt ataaaatgac taaaagttaa agttcatatt
    10621 tcaggcttta ttttcggctt tcttatttac gttattaagg gccagatcag tcagtttgat
    10681 gtcggtagcc ttttcttctt ccagggtttc tttcagaagc ttcgctgctt tacgataacc
    10741 taattgttca gccagcgtcg ctaaggtccc gtaactggcg atctcatagt gctcgacttt
    10801 ctgtgcagcg gcaatcagtg cggcatcacg cacttcgttt ttctcggtgc tttcgatgac
    10861 ctcattagct tcttcaatga gaccttccat tgccacacat ttcatgcgct taattttcag
    10921 gttcgattcg gactccacaa cttgatcaat acgttcaatc tgtccatgag tttcctcgag
    10981 gtgcgcatga aaagcctgac ttaatttttc atttgatgtt gctcttgcga gttttgccag
    11041 tgcccgggtt aattgttttt ctgcgctgta ggtatctgaa agcaggtgaa taaatacatc
    11101 ttcaatggtc ttcatattca tatttttctc cagtgaaatc actgcgagcc atacggcccg
    11161 cagatgcaat tatctttatc agctgatatt aataattaac ggtgaatcag gattttcctg
    11221 atttattacc accgctttgt tgaccgcctt ttttacccgc ttcggatgcg cgttgcggat
    11281 catttttaaa attaccgccg ctatgctgac cgcctttacg gcctgcgtcg gatgccttct
    11341 cacggtcttc ggcgaaattt cctgaaccac cacgatgttc ggccatgtta tttctcccgt
    11401 tgcgttgcat tgtttgatta atatgagtgt tgtgtgtcga cactcattaa aattagtcgc
    11461 taatgagaat tagtcaaatt aagcgcaacg agaagataga gggaaaatat attttgagga
    11521 acattctgga tatattaaca attacctgag gaataagtga cttagagaaa attattgatt
    11581 ttactggtgt tatgttgcgg gattaatttg tttgattata ttactgttgg gcggaaaaat
    11641 gacgtaagtt gacgttcgac cggggtaagc gaaacggtaa aaagataaat attaaatgaa
    11701 tttaggattt ttccggcttc attaaagaaa gttaaaatgc cgccagcgga actggcggct
    11761 gtgggattaa ctgcgcgtcg ccgctttcat cggttgtaca aaaactttca gtgccgccag
    11821 cattttctct ggctcattaa tatgttgctc gatgatttta acaatggccg aaccagaaat
    11881 cgcgcccgca gctcctgcat caatcgctgc ttttacctga tccggggcgg aaataccaaa
    11941 cccctgcaat ggaggtgcag cgttgtactc tttcagcttc gcaaccagat gattgagggg
    12001 taacgcggcg cggttttctg cgccggtcac gcccgctcgt gacagcaaat aggtgtaacc
    12061 acgaccgtaa gaggctatct ggcgcagcag gtcgtcatcg gcatttggcg ggcagatgaa
    12121 gataggtgcg acattatggc gcaacgcggc ctggcggaag ggcgcggact cttcaattgg
    12181 cacatcggca accagcaccg aatcgacgcc gactttctcg cactgggcat aaaactcatc
    12241 aatgcctttg ttaaacacca gattggcata catcaacagg ccaatgggaa tggtcgggtg
    12301 tttctggcga atcagtgcca gcatttcaaa acattgtgcc ggagtcacac ctgccgcaaa
    12361 ggcgcgcaga gtggcgtttt gaatcgtcgg gccatccgcc agtgggtcgg agaaggggat
    12421 acctaactcc agcgcgtcag caccggcttc aattagcgta tcgataattt tcaatgactg
    12481 ctcaatgccc ggatcaccga gcgtgacgaa aggaacgaat gcgccttctt tgcgctcctt
    12541 caactgggca aacagagatt cgtagcgttc catcagattt cccctcgtgc tttcaaaata
    12601 tcgtgaacgg tgaagatgtc tttatcgccg cgaccggaaa ggttaaccac cagtagctgc
    12661 tctttatccg ggttttcgcg catcattttc aacgcatggg ccagggcgtg ggaggattcc
    12721 agcgccggga tgatcccttc gtgcaggcac agcgttttga aggcttcaag ggcttcatca
    12781 tcggtaatag acacgtaatc agcgcgtcca gtgctgttaa gatacgcgtg ttgtgggccg
    12841 acagacggga aatccagtcc ggcggagatg gagtaagatt cttcaatctg cccgtcttcg
    12901 gtttgcatca tcggcgcttt cataccgaaa tagatgccca cgcgaccatg ttttaacggt
    12961 gcgccatgct cgccagtttc gataccgtga ccacctggct ccacaccaat caggccgacg
    13021 ttggtttcat tgatgaaatc agcaaacatg ccgatggcat tcgaaccgcc gccaacacag
    13081 gcgataacgg catccggcag gcgaccttct ctttccagaa tctgcgcttt ggtttcttcg
    13141 ccaatcatcc gctgaaactc acgcacaatg gtcggataag gatgcgggcc agctgcggtg
    13201 cccagcatat agtgcgcggt ttcgtaacta ccggaccagt cgcgcagcgc ctcgttacag
    13261 gcatctttca gcgtcgcgga accgctatgc accgggatca cttccgcacc cattaagcgc
    13321 atacgaaaaa cgttaggcga ctggcgttct acgtctttgg cacccatata aatacggcat
    13381 ttcaggccga gcagggcgct ggcaagggcc gacgccacgc catgctgacc ggcaccggtt
    13441 tcggcgatga tttcggtttt acccatccgc ttcgccagta aagcctgacc gagcacctgg
    13501 ttagttttat gcgcgccgcc gtgcagcaaa tcttcacgct tgagatacag agtggtgttc
    13561 gtcccggctg taatgttctg gcatttggtc agcgcggttg gacgcccggc atagtttttc
    13621 agcaggtcgt tgaactgagc ctgaaattca ggatcttttt gcgcactgac aaaagcttct
    13681 tccagctggc gcagagcagg catcaggatt tgtggcacgt acatgccgcc aaactcacca
    13741 aaataggggt taagtaatgt tgtcattgtt cctttcctta atatgcgcgc agcgtctgga
    13801 aaaccgaggc caaaagacgt gcgtctttga tgcccggttg cgactctaca gcagaattaa
    13861 aatcaagtcc ggcgcagccg gtttgtgccg cttccacgca gttatctgcg cctaagcccc
    13921 ccgccagcag aacgttgcca agcgattgac catttaatag tgaccagtcg aaacgttgcc
    13981 cgcttccacc ctggccgttg tctaaaacat atttatcaac gtgctgaagc tcgcgggcgg
    14041 gcagggtttc accgacgctt aatgctttcc agatggcaac gtgtgctggc agagcttcac
    14101 gcagcgtatc gatatacagc tgatcttcat taccatgcag ttgcactgcc gccagcgata
    14161 acaccttagc tttgtccacc acatcggcaa tatcgtgatt gcggaacacg ccaacatact
    14221 gcaacggtgc cgcagccatc acttcctgcg cctgttcaac gttgacgcaa cgcggtgatg
    14281 tcgcaacaaa aatcaaccca ccgtaaatcg cgcccgcgtc ataagctgct ttagcatctt
    14341 gcccacgcgt caggccacat actttattct cacccagcaa cacccggcgc acggcggcgt
    14401 gcaaatcgtc atgggccatc aacgccgaac caatcagaaa accgttagcg aagtggctta
    14461 actcgcgcac ctgagcgtaa gtattgatgc cggattcgct gattaccgtt acgttgtgcc
    14521 ccagtttcgg cgcaagctcg cgggtacggt tgagatcaat cgacaaatcc cgcagatcgc
    14581 ggttgttgat gccaacgacc tttgccccca atgcaatggc acgctccagt tcctcttcat
    14641 tactgacttc ggtcagcaca cccatctcca gactgtgggc gacggcggca agctggcgat
    14701 attgttcgtc atccagtact gaaagcatta ataagcaggc atcagcctgg taatagcgcg
    14761 ccagatagat ctggtaagga tcgataatga agtctttaca taaaatcggc tgcggggcga
    14821 tttggctgac gatggggagg aaatcaaagc tcccctgaaa atatttctca tcagtcagca
    14881 ccgaaattgc cgaagcgtaa tgtttataaa tggcggcaat gcgtgctgga tcgaaatcat
    14941 cacggatcac gccttttgac ggcgacgctt tcttgcactc cagaataaac gccgtgcgcg
    15001 caccctgtag cgcatcataa aaatgtcgcg tgctcggctg aacctcattc tgaaaactgg
    15061 ccagcggttg ctgctgtttg cgggtttcta cccaaatcgc cttgtctgcg acgattttcg
    15121 ctaaaacggt ttgcatcatt taccctcgtg ccgccagcgc ggtaactctg tcgtaagcgg
    15181 aaccactgcg cagtacctca agaacggttt gcgcattggc ttgcagatct tcatggccat
    15241 gcaggcgcat taacatggcg acgttcgcag cgacggctgc ttcatgggcg gcgtcgcctt
    15301 taccttgtaa caaacgtgtt aaaatgtcac ggttttcttc cggtgttccg cctgccagtt
    15361 gctcctggtg gtagggtgtc aggccaaaat cttcagcggt caattgatag ctcttaattt
    15421 cgccgtcatg cagctcggca acgattgtcg gcgcgtgtaa tgaaacttca tccatcccgc
    15481 cgctgtgcac caccgccgcg cgttgatacc ccagcacgcg caaggtttcg gcaatcggca
    15541 gcaccagttc cggactataa acaccaatta acgccagcgg cggatgcgcc gggttaatca
    15601 atggccccag cacattgaac agggtgcggg ttttcagttg ctggcgaacc ggcatcgcgt
    15661 ggcggaatcc ggtgtgatac ttcggcgcaa agaggaaaca tacacctaac tcatccagcg
    15721 cctggcgcga tttatcggcg ttcatatcaa gattaatacc gaacgccgcc agcagatcgg
    15781 acgaaccaga tttactggag acgctacggt tgccgtgttt cgccactttc agcccacagg
    15841 ccgcggcgac aaacgcactg gcggtagaaa tattgatact gttgctgccg tcaccgccag
    15901 taccgacgat atcagcaaac agataatccg ggcgcgggaa cggcgcggcg ttttccagta
    15961 gcgcggttgc tgctccggcg atctcgttcg ggtgctcacc gcgaattttc atgctcacca
    16021 gcgccgccgc cagttgttcc ggcttcagct cgccacgcac caccgctgaa aacagctggt
    16081 ggctttcttg ttggctaagc gtctgcgcct gatacagttt ttccagaatc ggttgcagcg
    16141 tgttggctgg ctctagtttc tgctgcgccc aggccagcgt ttgttccagc aggcgagcgc
    16201 cctgggtggt gagaatggat tccggatgga actggaatcc acaaacgcga tccgcatcgt
    16261 gacgtactgc catcaccatg ccattaaaat gggcgttgat ggttaaaccg gccggaatgt
    16321 tactgccaac cagcgagtga taacgcgcca ccggcagcgg gtttgttaat ccggcaaaca
    16381 tcgcctgacc gtcatgttca atgctcgacg ctttaccgtg aagaatttcg cccgcctgac
    16441 cgacatagcc cccgtaagct tcgacaattg cctgatgtcc gaggcaaatg ccaataatgg
    16501 gcagcttgcc acgcaagcgg gtgaggagtt ccggcataca accggcttcg ctcggcacac
    16561 cggggccagg agaaagcatc agtaccggat tgctcatcgt cgccaggcgt tcaattaagg
    16621 tctgcgccgg aatatggttg cggtaaatca ccacattatg cccattgctg cgcaactgat
    16681 ctgccaggtt gtacgtaaaa gagtcgatat tatcgagcag cagaatgtca gccatcagaa
    16741 agtctcctgt gcatgatgcg cggtggcaat agcgcgcagt acagcgcggg ctttattacg
    16801 ggtttcgtcg gcttccgact gcggaacaga atcaaggact acgccagcac cggcttgcac
    16861 ggtggcgata ccgttttcca ccagcgccga gcggatcaca atgcaggtgt cgagatcgcc
    16921 atgcgcggtg aaataaccta ccgcgccgcc gtagctgccg cggcggcgac cttccgcctc
    16981 ggcaattaac tgcatagcgc gtactttcgg cgcaccactt aacgttccca tattcataca
    17041 ggcgcggtaa gcgtgcaggg cgtcgagatc gtggcgcagt tcaccgacca cgcgggagac
    17101 caggtgcatc acgtaagagt aacggtcaac tttggtgagg tcggcgacgt agcggctgcc
    17161 gggggtgcaa atgcgtgcca gatcattacg ggcgagatcc accagcatca gatgttcaga
    17221 aagctcttta tgatcggtac gcatttccag ttcgatgcgg ctgtcgaggt ctctgtccag
    17281 cgagccatcg gcacgacgac cgcgtggacg tgttccggca atcgggtaga tctcaatctg
    17341 gcggctggtg gcgtcatact tgagcgaact ttccggcgac gcgccaaaca gggtgaaatc
    17401 attatcctgc ataaaaaaca tgtacgggct gggattactc tttttcagca cgtaataggc
    17461 cgccagcggt gacgggcagg gcagagagaa acggcgagat ggcaccacct ggaaaatttc
    17521 tccagcgcga atcgcttttt gcaacaaacg cactacgcca ccgaactctt catcgctctg
    17581 attacattca caacgcatat gcggcacgga aaccactggc agcggcggcg cggcttcggt
    17641 cagttgctga cgtagttcgt tcaggcgagc agtgagacgt tgtttttctt cttcattcgg
    17701 agcaaacagg ctggcctgaa tgcgggtgct ttttttctga tggtcaatca ccatcagagt
    17761 ttcagcgaga taaaaacaga aatcagggca gttattttcc gctgacagtt gcggtaaatc
    17821 ttcaaatccc gccacaaggt cataagagaa caggccgccg aagaacattg cttctcgttc
    17881 ttccttcggt acattcaaca gattctgcaa taaacggaaa gcgtcaaaaa ccgaaaggga
    17941 gcataagcga gcgtcttcat ccagcagtgg actgacaggg gggaagcgca gcacacggca
    18001 gtttggtaat tgttcatttt ccacacccgc aggcagggcg ttatccagta gtgtcagcag
    18061 ggcttcgccg ttgccggaaa gtgcctggat tgtgacagtg tcacctaaag ctgtaatgcg
    18121 cagcgcactg tctaccagca gcaggctttt taaatcatct ttgctgtcga tatctgcgga
    18181 ttccagcagc agcgttgccg gacgatcccc acacaactgg tgaaaaagcg cggtgggatt
    18241 gtcgcgataa gcgccttcgc aggttagcag ttcgagagtc ggtttttgtg tttgcattgt
    18301 tattctctaa ttttgttcaa aaaaaagccc gctcattagg cgggctgggt atctgattgc
    18361 tttacgcatg gtgaatacac tgcccgtttc aggaagtgcg ccaccaacct ttcagtacga
    18421 aaattgcttt cattgtcgat acccttttta cgtgaacttg cgtactagtt aactagttcg
    18481 atgattaatt gtcaacagct catttcagaa tatttgccag aaccgttatg atgtcggcgc
    18541 aaaaaacatt atccagaacg ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac
    18601 gacctgcaca gccataccac agcttccgat ggctgcctga cgccagaagc attggtgcac
    18661 cgtgcagtcg agatgcgcgt cggcaccctg gcgatcaccg accatgacac cacagctgca
    18721 atcgcgccag caagagagga aatttcacgt tcgggactgg cgttgaatct tattcccggc
    18781 gtggaaattt ccacggtctg ggaaaatcat gaaattcata ttgtcgggct gaatattgat
    18841 attactcatc cgctaatgtg tgagttcctc gcgcagcaga cagaacggcg caatcagcgg
    18901 gcgcagctga ttgccgaaag acttgaaaaa gcgcaaatac ctggggcgct ggaaggcgca
    18961 caacgactgg cgcagggcgg cgcagtgacg cgcggtcatt ttgcgcgttt ccttgttgag
    19021 tgcggcaaag ccagttcaat ggcggatgtc tttaaaaagt atctggcgcg cgggaaaacc
    19081 ggatacgttc cgccacagtg gtgtacaata gaacaagcta ttgatgtcat tcatcattct
    19141 ggcggtaagg cggtgctagc tcatcctggg cggtacaatc ttagtgctaa atggctgaaa
    19201 agattggttg cgcattttgc cgaacaccac ggtgacgcga tggaagtcgc gcagtgccag
    19261 caatcgccca atgaacgtac ccagctggcg gcccttgcgc gtcagcatca tttatgggca
    19321 tcacaaggat ctgattttca tcagccatgc ccgtggatcg aactgggtcg taaactctgg
    19381 ctgcccgcag gcgttgaagg cgtctggcag ctatgggaac agccgcagaa caccacagag
    19441 agggaattat gagccagttt ttttatattc atcctgataa cccacagcaa cgtctgatca
    19501 accaggcggt ggagatcgtg cgtaaaggcg gggtgattgt ttatccaact gattccggct
    19561 atgcgctcgg ctgtaaaatt gaagataaaa acgcgatgga gcgtatttgt cgtattcgcc
    19621 agctgccgga cggtcacaac tttaccctga tgtgtcgcga tctttctgaa ctctcgacct
    19681 attcatttgt tgataacgtt gcgtttcgtt taatgaaaaa caacacgccg ggcaactata
    19741 ccttcatctt gaaggggacg aaggaagtgc cacgccgtct gttgcaggaa aaacgcaaaa
    19801 ccatcggtat gcgtgtgcct tctaacccga ttgcgcaggc gttgcttgaa gcactgggcg
    19861 aaccgatgct ttccacttcg ctaatgctgc caggcagcga atttaccgaa tccgatcccg
    19921 aagaaattaa agaccgtctg gaaaaacagg tggatttgat tatctacggc ggctatctcg
    19981 gacagaaacc gacaacggtt attgatctta ccgatgatac gccggtcgtg gtgcgtgaag
    20041 gcgtaggtga tgtgaagcct ttcttataag tgcgtaactc tgtatactac gcggccttaa
    20101 aatggcgcta tacggcgcca ttgattctcc tgttcgacgc ctgtgaaggc gacaccaaaa
    20161 ggaagctcta tgagcgaaaa attacagaaa gtgctggccc gcgctggcca cggctcccgc
    20221 cgtgaaatcg aatctattat tgaagccggt cgtgtgagtg ttgatggcaa aattgctaaa
    20281 ctcggcgatc gcgttgaagt tacccctggg ctgaaaattc gtatcgatgg tcatctgatt
    20341 tcggtacgtg agtccgctga acaaatttgt cgcgttctgg cctattacaa accggaaggt
    20401 gagttgtgta cccgtaacga tccggaaggg cgtccgacgg tgtttgaccg tctgccaaag
    20461 ctgcgtggcg cacgctggat tgccgtaggt cgtctggacg ttaacacctg tggtctgctg
    20521 ctgttcacca ccgatggtga actggcaaac cgtttaatgc atccaagccg tgaagttgaa
    20581 cgtgaatacg ccgtgcgtgt atttggtcag gttgacgacg cgaaactgcg tgatttgagt
    20641 cgtggcgtgc aactggaaga tggtccggca gcttttaaaa ccatcaagtt cagcggcggc
    20701 gaagggatca accagtggta caacgtgact ctgaccgaag gccgtaaccg tgaagttcgt
    20761 cgtctgtggg aagcggttgg tgtgcaggtg agccgcctga tccgtgttcg ttacggtgat
    20821 atcccactgc caaaaggtct gccacgcggt ggctggacgg agctggacct cgcccagact
    20881 aactatctgc gcgaactggt ggagcttccg ccagaaacca gctctaaagt cgctgtagaa
    20941 aaagaccgtc gtcgcatgaa ggctaatcag attcgtcgtg cggtgaaacg tcacagtcag
    21001 gtgagcggcg gtcgtcgttc tggcgggcgt aataacagtt aatcaaagta tgccggacgt
    21061 catatccggc atttttacag attaataatc aatccctatt tgcgccttaa caccggcatc
    21121 aaacgcatgt ttgatggggc gcagctcact taccgtatcc gccagttcaa gaatatcccg
    21181 atgacagcct cgacccgtga taatcaccgt ctgttgatgt ggacgttcat ttaacgcctg
    21241 caccacttct tccagtggca aatagtcata cg
//
LOCUS       DAEVZA010000089        21020 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEVZA010000089 DAEVZA010000000
VERSION     DAEVZA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.089"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..945)
                     /locus_tag="LAF92_003293"
     CDS             complement(<1..945)
                     /locus_tag="LAF92_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI9792249.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNEL"
     gene            complement(1038..1685)
                     /gene="rnt"
                     /locus_tag="LAF92_003294"
     CDS             complement(1038..1685)
                     /gene="rnt"
                     /locus_tag="LAF92_003294"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBI9792250.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(1788..2195)
                     /gene="gloA"
                     /locus_tag="LAF92_003295"
     CDS             complement(1788..2195)
                     /gene="gloA"
                     /locus_tag="LAF92_003295"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBI9792251.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKVELIEEKDAGRGLGN"
     gene            complement(2276..3373)
                     /gene="nemA"
                     /locus_tag="LAF92_003296"
     CDS             complement(2276..3373)
                     /gene="nemA"
                     /locus_tag="LAF92_003296"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBI9792252.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(3410..4009)
                     /gene="nemR"
                     /locus_tag="LAF92_003297"
     CDS             complement(3410..4009)
                     /gene="nemR"
                     /locus_tag="LAF92_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBI9792253.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            4112..4351
                     /locus_tag="LAF92_003298"
     CDS             4112..4351
                     /locus_tag="LAF92_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBI9792254.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            4400..5296
                     /locus_tag="LAF92_003299"
     CDS             4400..5296
                     /locus_tag="LAF92_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBI9792255.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            5377..5898
                     /gene="sodC"
                     /locus_tag="LAF92_003300"
     CDS             5377..5898
                     /gene="sodC"
                     /locus_tag="LAF92_003300"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBI9792256.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(5899..7911)
                     /locus_tag="LAF92_003301"
     CDS             complement(5899..7911)
                     /locus_tag="LAF92_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI9792257.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(7911..8768)
                     /locus_tag="LAF92_003302"
     CDS             complement(7911..8768)
                     /locus_tag="LAF92_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI9792258.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(8771..9007)
                     /locus_tag="LAF92_003303"
     CDS             complement(8771..9007)
                     /locus_tag="LAF92_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBI9792259.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            9208..9642
                     /gene="slyA"
                     /locus_tag="LAF92_003304"
     CDS             9208..9642
                     /gene="slyA"
                     /locus_tag="LAF92_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBI9792260.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(9689..10156)
                     /gene="slyB"
                     /locus_tag="LAF92_003305"
     CDS             complement(9689..10156)
                     /gene="slyB"
                     /locus_tag="LAF92_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBI9792261.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            10430..11539
                     /gene="anmK"
                     /locus_tag="LAF92_003306"
     CDS             10430..11539
                     /gene="anmK"
                     /locus_tag="LAF92_003306"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBI9792262.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGINGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            11637..11966
                     /gene="mliC"
                     /locus_tag="LAF92_003307"
     CDS             11637..11966
                     /gene="mliC"
                     /locus_tag="LAF92_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBI9792263.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            12025..12681
                     /gene="pdxH"
                     /locus_tag="LAF92_003308"
     CDS             12025..12681
                     /gene="pdxH"
                     /locus_tag="LAF92_003308"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBI9792264.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            12810..14084
                     /gene="tyrS"
                     /locus_tag="LAF92_003309"
     CDS             12810..14084
                     /gene="tyrS"
                     /locus_tag="LAF92_003309"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBI9792265.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            14143..15006
                     /gene="pdxY"
                     /locus_tag="LAF92_003310"
     CDS             14143..15006
                     /gene="pdxY"
                     /locus_tag="LAF92_003310"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBI9792266.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(15050..15655)
                     /gene="gstA"
                     /locus_tag="LAF92_003311"
     CDS             complement(15050..15655)
                     /gene="gstA"
                     /locus_tag="LAF92_003311"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBI9792267.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(15761..17263)
                     /gene="dtpA"
                     /locus_tag="LAF92_003312"
     CDS             complement(15761..17263)
                     /gene="dtpA"
                     /locus_tag="LAF92_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBI9792268.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(17874..18509)
                     /gene="nth"
                     /locus_tag="LAF92_003313"
     CDS             complement(17874..18509)
                     /gene="nth"
                     /locus_tag="LAF92_003313"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBI9792269.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(18509..19204)
                     /gene="rsxE"
                     /locus_tag="LAF92_003314"
     CDS             complement(18509..19204)
                     /gene="rsxE"
                     /locus_tag="LAF92_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBI9792270.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(19208..19828)
                     /gene="rsxG"
                     /locus_tag="LAF92_003315"
     CDS             complement(19208..19828)
                     /gene="rsxG"
                     /locus_tag="LAF92_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBI9792271.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(19832..20890)
                     /gene="rsxD"
                     /locus_tag="LAF92_003316"
     CDS             complement(19832..20890)
                     /gene="rsxD"
                     /locus_tag="LAF92_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBI9792272.1"
                     /translation="MVFRIASSPYIHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(20891..>21020)
                     /locus_tag="LAF92_003317"
     CDS             complement(20891..>21020)
                     /locus_tag="LAF92_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HBI9792273.1"
                     /translation="LEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         5299 a         5315 c         5435 g         4971 t
ORIGIN      
        1 aagctcattt aatcgtgccg tcagtttttc cgccagccca cgtgaattag taaagacaat
       61 ggtcgagcga tggcgcaaca cttcatcaag gatacccgtt tcaatatatg gccagatgga
      121 gccttcccgg ccggcatggc tgtcttcgcc ggtgccgctg gcgaccgatg agacatcatc
      181 catattggcg accggtacga caattcgtat ctgcggatgg cgcattgcgg gcgggttgac
      241 taccgtaacc gggcgatcgc caccaagaaa tgctgccaca tcgctggctg agcgcacagt
      301 ggcagaaagg ccaattcgct gtgctgaggt gtggagcagc gcatcgagcc gctccagact
      361 taacgccaga tgcgcaccac gtttactgcc cgctaccgcg tggacttcat caataattac
      421 cgtttcgacg ccgcgtagcg tttcgcgcgc gcgggaggtc agcatcagat agagtgattc
      481 gggtgtggtg atcagaatat ccggcggatt acgggtgagt ttgctgcgtt cctgtgcagg
      541 cgtatcgcca gtacggatcc ctacgcgaag attgacttcc gtttcgccgc gccgccgccg
      601 ttcatcggca atacctttca acgggagctg caagttgcgc tgaacgtcgg tgcccagagc
      661 ttttatcggt gaaatataga ggatgcgtga ggttttacgc ttatgcgcct cgcgggtatc
      721 ttcgccgcct tcgcggaaga gccgatcgag ggcgtagaga aatgctgcca gcgttttccc
      781 ggagccggtc ggtgcaatca ccagcgcatg ttcgcttcgc gccgccacat gccaggtttg
      841 cgactggaca gcggtcggct gtttaaaggc gcgaagaaac cagtcgcggg tcgccggtga
      901 aaacacgtcc ggcaggagcg atgaagggtc tggattatct gccattaacg ctattccccg
      961 tgaatatcat gcttgatgcg tggaatagcc tcactataga tgcctgaatc attgcatgtc
     1021 atcaggcatc gactcgatta cacctcttcg gcggcaggta gcggccagcc tcccagacgt
     1081 ttccagcggt tgacgatttc acaaaacagc acagcagtgc gttcggtgtc gtacagcgcg
     1141 gagtgcgcct gggtgctgtc gaagtccatg ccagcggtct ggcaagcctt tgacaatacg
     1201 gtttgtccga gtgccagccc ggccagtgca gcagtgtcaa aagtggcgaa agggtggaac
     1261 gggttacgtt tcagtgaggc gcgttctgcg gcggccatca taaagctgtg atcaaaattg
     1321 gcattgtgcg ccaccataat ggcgcggtta cagccgctcg ctttaatacc tttacgtaca
     1381 actttaaaaa tttcgtgcag cgcctcgtat tcgctgaccg cgccgcgatc gggatcgttc
     1441 gggtcaatgc cgttgaaggc gagggcctct ggctgcaaat ttgcgccgac aaatggttcg
     1501 acgtggaaat gtaatgtggt gtccggcatc agccagcctt gttcatccat tttcagggtg
     1561 atggcggcaa tctcaagcag cgcatcggtt ttggcgttaa atccggctgt ttcaacatcg
     1621 atcacaacag gataaaaacc acgaaaacgg tcgcacagac cggtaagttg agcgttatcg
     1681 gacatctgat tctcttaata cggataaatt gcagcgcgca ttatgacaaa tattacttgt
     1741 cgatgcagta aagatgcggg cgcgatgagt tcacgcccgg caggagatta gttgcccaga
     1801 ccgcgaccgg cgtctttctc ttcgattaac tcaactttgt aaccgtccgg atcttccaca
     1861 aacgcgataa ccgtagtacc gccttttacc ggacccgctt cacgggtcac gttacccccg
     1921 ttttgacgga ttttttcgca cgcttcagcg gcgttatcta cgctaagcgc gatgtgacca
     1981 taagcagtgc cgagttcgta tttatccacg ccccagttgt aggtcagttc aatcaccgct
     2041 tcttcggttt ccgggccgta gccaacaaac gccagtgagt atttgtattc cgggttttcg
     2101 ctggtacgca gcagtttcat gcccagcact ttggtataaa aatcgatgga gcgttgcaaa
     2161 tcgccaacgc gcagcatggt atgaagaaga cgcatctttt tatcctcaaa atggctaaca
     2221 gattcaaaat gttgttttag tatagcggcg gctttacgcc gctcgcaatg ttggattaca
     2281 acgtcgggta atcggtatag ccttccgcgc cgccaccgta gaaactttcg gcacgctgtg
     2341 ggttaagctc agctttgcgc tgcaagcggg cgaccagatc cgggttcgca atccagtcac
     2401 gaccaaatgc caccgcatca attaaccctt tgccgatcag cgtttcagct ttttctactg
     2461 tgtatgcacc tgcgccgata atcggaccgt ggaaacgggc gcgtactttt tcgcggaacg
     2521 catcagtata cggttcaccc cccgcccaat ctggttctga catatgcaga taagcaatgc
     2581 cgcgtttacc cagttgttca atcagataca gtgcatcggc ttcttcattc gggccgttat
     2641 ccgtgttctg gaaagtaccg attggcgaaa tgcgaatgcc aatgcgatcg gcaccccatt
     2701 cttcaatccc ggcatcgacc acttccagta ccagacgtgc gcgattttcc acgctgccgc
     2761 cgtactgatc ggtacgatgg tttgaagaag gagaaaggaa ctgatgcagc aaataaccgt
     2821 gagcagagtg gagctctacc agatcaaaac cggcttcacg cgcgttagca atggcctgac
     2881 ggaaatcatt gacgatacct ggaatctctt ccagttcaag cgcacgcggc atggatgttt
     2941 caacacggat cgcctgacca ttttcatcgc gcagagaagt acgtgttccc gcgctaagtg
     3001 ctgaaggcgc taccggtgcc tgaccgccag gttgcaggct ggcgtgagaa atgcgtccgg
     3061 tgtgccacag ctgcacggcc atatgaccat tttcagcatg aacgccagcg gtgatttttt
     3121 tccatgcggc aatttgctcc ggactatgga tgccaggcgc acctgcatat ccttttgcct
     3181 gggcagaaat ttgcgtggct tcactaataa tcaaaccggc actggcacgt tggcgatagt
     3241 attccgccat caacggggta ggaatgtcac ccggttcaat actgcgcaga cgcgtcagcg
     3301 gtgccataaa aatacggttt gccgccgtga tcgcgcccac tttcagtggg gaatacagtt
     3361 tttcagatga cataaaggct cccggtcgtc tggtgataaa aaatgcctgc taaacggcag
     3421 gcgtcgcaat aatgtttttt acatgggcca gcgcgttttc cagtggctcg aaactgcgcg
     3481 aaattttggc ctgcagattc gcacccagcc atagtgcgta aagcacttgt gcctgttgta
     3541 gcggttcgcc acaaaaggtt aaacaatggt tctcacggcc attttccagc gcctgagaga
     3601 gcagggcgat cacgccgcga gcacctttat ccatcgcgct gcgcatatct tctgacagat
     3661 cgcacacttc ggcagagagt tttactgtca ggcaaccact gatggttcca tgttggcaaa
     3721 actggttcag tgtttgctgg taataagcca gtatgcggtc gcggtagtta ccttcgccgg
     3781 attgcagcaa ctcagtcagt cgctggtgat atgcggcgta atgacgctca agcatggcaa
     3841 cgccaaacgc ttctttagag cgaaagtagt gatagaagga ccctttcggc acttcagcgg
     3901 tttttagtaa ttcgcttagc cccatcccgg tgaatccacg ttgcaggcaa agttgctcgc
     3961 ccgtcgccag gagatgttcg cgagtatcat gttcggtgtg tttgttcatg cgttggagtg
     4021 tagtagacca gtcggtctag tgcaagcaaa gttgctactt tggacattaa cagcttcaat
     4081 aatggtaata atcttatctg aggagggcga agtggcggag caattagagt tctttcccgt
     4141 ccagagcccg tgccggggaa tttgccagtc tgacgaacgc gggttttgtc gcggctgttt
     4201 tcgtagtcgg gatgagcgtt ttaactggaa taaaatgagc gatggcgaaa aacaagaggt
     4261 gctgcgcctt tgtcggcagc gattaatgcg caaattacgc gcaaataagc cagcgtcatc
     4321 agacgaaccg gagcaaccat cactcttttg aacctaatct tgcgtatact cctctcatat
     4381 ctgataagag gaagcgatta tggttcagcg tattactatt gcgccgcaag gcccggagtt
     4441 ttcccgtttt gtgatgggct actggcgatt gatggactgg aatatgtccg cccgccagct
     4501 ggtcagtttt attgaagagc atctggatct cggcgtgacc accgtggacc atgctgatat
     4561 ttatggtggc tatcagtgcg aagcggcgtt tggcgaggca ctgaaactgg cacctcacct
     4621 gcgtgaacgg atggaaatcg tcagtaaatg cggtatcgcg acgaccgcgc gtgaagaaaa
     4681 cgtcattggt cattacatca ctgaccgcga tcacatcatt aagagcgccg aacagtcgct
     4741 aattaatctc gcgaccgatc atctggatct gctgttaatc caccgaccag acccgttaat
     4801 ggatgccgat gaagtggcgg acgcgttcaa acatctgcat cagagcggca aagtgcgtca
     4861 ttttggcgta tcgaacttta cgcctgcgca atttgccctg ttgcaatcac gtctgccgtt
     4921 tacccttgcc actaatcagg tggaaatatc cccggtgcat cagccgttac tgctggatgg
     4981 cacgctcgac caactacaac aactgcgtgt tcgtccgatg gcgtggtcct gccttggtgg
     5041 tggtcgtctg tttaatgatg attatttcca gcagctgcgt gatgaactgg ctgtggtggc
     5101 agaggagtta aacgcgggct cgattgaaca ggtggtttac gcctgggtat tacgtttacc
     5161 atcgcagccg ctgccaatta tcggttcagg taaaattgag cgcgtacggg cagctgtcga
     5221 agcagaaaca ctgaaaatga cccgtcaaca atggtttcgt atccgtaaag cggcactggg
     5281 gtacgacgta ccgtaagcac ttttaggaat agccgccgtt caaaaatgtg tcactggttt
     5341 acacttattc aggaatgcac aatgaacgga ggtcctatga aacgttttag tctggctatt
     5401 ctggcgctgg ttgttgcaac cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc
     5461 gtcacgtcgc aaggggtagg gcagtcaatt ggtagcgtca ccattactga aaccgataaa
     5521 ggtctggagt tttcgcccga tctgaaagca ttaccccccg gtgaacatgg cttccatatt
     5581 catgccaaag gaagctgcca gccagccacc aaagatggca aagccagcgc cgcggaatcc
     5641 gcaggcgggc atcttgatcc acaaaatacc ggtaaacatg aagggccaga aggtgccggg
     5701 catttaggcg atctgcctgc actggtcgtc aataatgacg gcaaagctac cgatgccgtc
     5761 atcgcgcctc gtctgaaatc actggatgaa atcaaagaca aagcgctgat ggtccacgtt
     5821 ggcggcgata atatgtccga tcaacctaaa ccgctgggcg gtggcggtga acgctatgcc
     5881 tgtggtgtaa ttaagtaatt aagaggccag cgtaccttgc ggtggtgctt gctcaagttg
     5941 cgaaagcgag cagtacagcc gccagacaat tgccgccagc tcacgggcgg caggttgatg
     6001 atgacgggca aggctgtcgc aaatccgctg taattcttca agtgtggccg ccagtgattt
     6061 ttgctgaacg ccacgctcac tcatcactcc ccgcaacaat gaaatacagt tatcccgtac
     6121 tcgcgataac ggatcggaac gcgattccca gtcgcgcaat tgccagacaa catgagaaca
     6181 gttcagcagc actacacccc agcgtaataa ccaacggcga gccagcgcat cctggctgtt
     6241 actcaactga ctgacgtgat gataagtgag cgattcaaat tcgctttcac tcagtgttgg
     6301 atggcggctt agctgatcga caaaatcccg gcgcagcgcg cgaatatggc ggcggctttt
     6361 acgagcatcc gatcctggac gcagaatggc gaacgctagc caagccagcg caacgccaac
     6421 gatttttgcc agattatcgt taagaaaatc agcaaaatca tacaccgggg gattagtgac
     6481 agcgataaac gaacccataa aaacaatcag ttgcccccac agtgcggcaa atttgggcat
     6541 ctgcaattta agaagctgca ttgtcgccag cagtggaaag agaaacagta aaaattgcca
     6601 cagatcgcta atctggacca tcaggccaaa tttgaccaca aagctgaata gcgaaagtaa
     6661 caccagcgtg cgcatcagca gtgacaacga cttaaacggt gctgcgacgg cggagtagag
     6721 tacgcagcta attgctgcca gcgttaatgc attggcaccg gcatcccatt gcgatgcaat
     6781 actccatgcg ccaatcatca tcaacgtaca aaatgtacgc agcccgctcc acatagcttc
     6841 ggcgttgtcg gtatgacgag ccagagcgga tgtccgtgcg agtctggcat gatcatctac
     6901 accgctttgc agacgatgta attcctgact actttgcaga taaaggcggc aaaaataacg
     6961 tagtcgctgc cagaaggcga cgtgccggta gtcggcgacg ttggtcgggc gtagcggggc
     7021 gataatacgt gcgacggtgt aagcatctgt ttgcgaactg gcgagcgccg tcagcaattg
     7081 ttcgagaatt tctcgcgtgg cacctggcgg tgagggccag ttgagcaaca tacgtcgcag
     7141 gctggagatg acactggtca tacgtaattg ctggtggagt agcgcattaa ggcgcgcgtt
     7201 ttgctggcga aaacgatagt ggctccagaa agcctggata cgcagcaaat tcatggtcag
     7261 tatctgccca atcaccccct catgtgctgc acgaatggca tcgtttgttt caggctgcca
     7321 gagtaaactg gcatgttcca gtaatcgggc gtgcatgttt ttcaatgcgg ttaaaagggc
     7381 tgtagcatcg gaactgctag gcaggatcat catcatcatg ccgccgcaca aaataccgac
     7441 tatcacctcg caaacgcgcg cctgagcgat atcccacagc tggctggcct cagtaatatt
     7501 aaccatcgga aaggcgatga tggcagccgt gtagcccgcc agttgaaatg catacgcgac
     7561 gttattcgtg aagtgcgcac aggcccaggt acaaaagcca agccacgccg acatgctcaa
     7621 tagaaaaaac cacggctcat tgagcgtatg cccggcaaga agcagtgccg caatggctcc
     7681 cagtaaactg ccagcgatgc gtccgaggct tttgctgatg acaccgccaa cggtgggaaa
     7741 gctaaccact gcagccgagg tcatcgccca atagggttca tccagattca aataataggc
     7801 aaccgtcagc gccagacaca tggcaatggt attgcgtaac gcataacgcc attgcgccag
     7861 cgtggccttg aaccagggca aattgcgcaa ggaccatgac gatgcgttca ttattgacct
     7921 acggcaatgc tacaggtggt gcccgacacc agcgtgacat cgcctggaac tttatccagc
     7981 gcaaagcgga cgggaacgcg ttgggcgaga cgaacccagg ggacgttagg tttcacatcc
     8041 ggaataaggc ttgagtcact ttcaacgctt tgatcataaa tcgcccgacc gatactggaa
     8101 acgtgacccc gtaacgtttt gttatcacta tagagggtaa tttgtgctgg tgcaccttcg
     8161 cggatatgtc gcaacttggt ttcttcaaaa taaccaatga cgtaaaacga gtggctgtcg
     8221 acaagggcaa acagcggttt cccggtgtca gcgtaatcgc cgatgcgggt agtgaggtta
     8281 gtcacccaac cactcaccgg ggcgcgaatt tccgtttgcg ccagttgcca ttgtgtctgt
     8341 ttcagcgtgg cctgtgcggc attaacactg gcctgcatcg ctttaacatt gaggttagcg
     8401 gtatcgagtt cttcggcaga aataaaattt tgtgagagat gacgacgacg attcgcttcg
     8461 ttattggctt ttgcgaggtc agattgcgct ttcgccagct gagcctgtgc gttcagctcg
     8521 gcgatctgaa agggcgtttt atcgatcgtg agcagaagat cccccgcgtt aaccagttgg
     8581 ttgtctttta tattcagctc aacaatacgt cccgacacct gtggggtgat agaaaccttt
     8641 tcggcgcgta ttttcccgtc gcgtgtccac ggagattgca tgtaataatt ccacagccac
     8701 catccggcaa gaaccgcaac tatcgctaca atgattgttg agaaatactt aattgtttta
     8761 attgacatag ttaccacgca atcagtattg ccagagcaag gcaaacgcaa atcgcaaaca
     8821 gcgataaatc cattaacaag ggatgccaga tgtcaccggc gtagatccag ccacgaagca
     8881 ggcggtgtac gacaagccag atgacgaatc caaacgcgaa ggctttgaaa aacggaggaa
     8941 aatagacgga cgcaccgaac accagatctt gtaagggcaa tcctgttgca ttgagcataa
     9001 acttcacaaa gtagattcct ttacgaccga atatattgcg ttagattaat aaatattctt
     9061 taagtgcgaa aaatttacgc gcaatttctg aaaaatatgt ctgacggtaa ccaaatgcag
     9121 caatacattt gttttagcaa tacaattgct gcacactatt ctaaaagccg cataatatct
     9181 tagcaagcta attataagga gatgaaattg gaatcgccac taggttctga tctggcacgg
     9241 ttggtgcgca tatggcgtgc tctgatagac catcgcctga aaccgctgga gttaacacaa
     9301 acccattggg ttacgttaca caatattcat cagttacctc cagaccagtc gcaaattcaa
     9361 ctggcaaaag cgattggcat cgagcagcca tcactggtcc gtactctgga ccaactggaa
     9421 gaaaaagggt taatttcgcg tcaaacttgt gccagcgatc gtcgggctaa acgtattaaa
     9481 ctgacggaaa aggcagagcc actgatcagc gaaatggaag ctgttattaa caaaacccgc
     9541 gcggaaatat tacatggcat ctccgcagag gaactggagc aactgattac gctcatcgca
     9601 aaacttgagc ataatatcat tgagttacag gccaaagggt gaaatgaagg gggcgtgtgg
     9661 tcagtaacct gaccacacgc aaacttattt agcgcggaga aacggtcacc tgactgccat
     9721 tgctggccag tacgacacgt tggcccggag agaaacgagt gttgccttgt ttctgtacca
     9781 ccatgatggt attaccatcg tctttacgaa tttccagctc gacaccctgc gttttgttca
     9841 ttgcactctg tacgccctga ccagctacgc cacctgcaac agcgcctgct gcagtagcca
     9901 gagaacgccc ggttccgcca ccaacagtat tccccaggaa accaccaaga acagcaccgc
     9961 caattgcacc gataacgttg gaatcatcac cgccctgaat ctgtaccgga cgtacgttaa
    10021 cgatggtgcc atagctgaca ttctgtactt gtttcgcttc agaagcggta taaacatccc
    10081 ctgacagggt gtcgttatta acacaaccga caagagacag acctaccatt gaaacaacca
    10141 atacgcgttt aatcattgaa aaatctcctg ttcaccatga aacgccacgc aagcatccct
    10201 catggtcaaa gtatatggca tatgtgaatc catggttaca tgttctacca aatatgagca
    10261 aatcataatg aaagtaggat caaccaatta taaacaaaac attcatgatg attattgatt
    10321 tgaagataat gttttactac caacggtaaa aatcggtcat tccgcatggt attcacgaaa
    10381 agtttatgtt tgagtggtgc gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg
    10441 ccgctttatt ggcgttatgt caggcaccag ccttgatggt gttgatgttg tgttggcgac
    10501 aatagatgaa caccgggtcg cacagctggc aagcttgagc tggccgatcc cggtgtctct
    10561 gaaacaggct gtactggata tttgccaggg ccagcagctt acactttcgc agtttggaca
    10621 gcttgatact caactcgggc gactttttgc tgatgcggtc aatgccttgc ttaaggaaca
    10681 aaacctgcag gcgagagata tagttgcgat cggctgtcac ggtcaaaccg tctggcatga
    10741 accgacgggc gtggcaccac acactttaca gattggcgat aacaatcaaa ttgtggcacg
    10801 caccggaatt acggttgtcg gtgattttcg ccgtcgcgat attgccttgg gaggacaagg
    10861 cgcaccgctg gtacctgcgt tccatcatgc cttgctggct cactcaaccg agcgacgaat
    10921 ggtgctcaat attggcggca tcgccaatct gtcactgctc attcctgggc agccggttgg
    10981 cggctacgat accggtcctg gtaacatgct gatggatgcc tggatctggc gtcaggccgg
    11041 taaaccttac gataaagatg ccgagtgggc acgggcgggt aaagttattc tcccactgct
    11101 gcaaaatatg ctcagcgacc catatttctc gcaacctgca ccgaaaagca ccggacgcga
    11161 atactttaac tacggctggc tggagcgcca tttacgccat tttccgggtg ttgatccccg
    11221 tgatgttcag gccacactgg cagaactcac cgccgtgacc atttctgaac aagttttgtt
    11281 gagcggtggc tgcgaacgat tgatggtatg tggtggaggt agtcgtaatc cgctactcat
    11341 ggcgcgtctg gcggcattac tgccaggcac agaagtcacc accaccgatg ccgttggcat
    11401 taatggcgat gacatggaag cattggcttt cgcctggctt gcctggcgga cgctggcggg
    11461 attaccagga aatctgcctt ccgtcactgg cgcaagccag gagacggtac tgggggctat
    11521 tttccctgct aacccgtgat atcagagtta actgaatttt tcttaccgcg tctgccgtta
    11581 cacttggaac attagggagg gcgtattgcc ctccagacca ggaaagtctt cgggaaatgt
    11641 ctatgaaaaa actgttactg atctgtttgc cggtgcttct caccggctgt agcgcgttca
    11701 atcaactggt tgagcacatg caaaccgcta cgctggaata ccagtgtgat gaaaaaccgt
    11761 tgacggtcaa actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac
    11821 tgcatctcaa acagggcatt tcagcgtctg gtgcacgtta cactgacgga atctatgttt
    11881 tctggtcgaa aggcgatgaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact
    11941 gtcagttaca aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc
    12001 cactgacaat ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat
    12061 ctgcgccgtg aatacaccaa aggcgggtta cgccgtcgcg atcttcccgc cgatccatta
    12121 accctttttg aacgttggct ctctcaggcg tgtgaagcca aactggcaga ccctaccgcg
    12181 atggtggtcg ctaccgtgga tgaacatgct cagccttatc aacgcatcgt tttactcaaa
    12241 cattacgacg aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa
    12301 attgaaaata atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg
    12361 atggtgatcg gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc
    12421 cgcccgcgtg atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc
    12481 cgcggtatcc ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg
    12541 ccattgccga gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag
    12601 ggcggtgagc atcgcctgca tgaccgcttt ttgtaccagc gagaaaatga tgcgtggaag
    12661 attgatcgtc ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg
    12721 attctggcac tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag
    12781 gtgcagtcgt tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc
    12841 aagagcgggg gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc
    12901 aaggcccgat cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc
    12961 atcttgttcc attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc
    13021 tggtaggcgg cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc
    13081 tgaacaccga agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt
    13141 tcctcgattt cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg
    13201 gcaatatgaa tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga
    13261 tgatcaacaa agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca
    13321 ctgagttttc ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg
    13381 gtgtggtgct gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc
    13441 tgacccgtcg tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcactaaag
    13501 cagatggcac caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa
    13561 ccagcccgta caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct
    13621 tcctgaagtt cttcaccttt atgagcattg aagagatcaa cgccctggaa gaagaagata
    13681 aaaacagcgg taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg
    13741 ttcacggtga agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt
    13801 ctttgagtgc gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg
    13861 ttgagatgga aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt
    13921 cccgtggtca ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac
    13981 agtccgatcc tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac
    14041 tgcgtcgcgg caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag
    14101 ggggagtgag aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc
    14161 gctatccagt ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttcctatg
    14221 cgccgcctgg gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa
    14281 tacggcaaat ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc
    14341 attgccgcca ttgataaatt acacacctgt gatgccgtgt taagtggcta tctgggatcg
    14401 gcggagcagg gtgaacatat cctcggtatc gtccgtcagg tgaaagccgc gaatccgcag
    14461 gcgaaatatt tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg
    14521 ggtgtcgcag agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat
    14581 ctggttgagc tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg
    14641 gcagcgcgcg aactcattgc gcaagggcca caaattgtgt tggttaaaca cctggcgcga
    14701 gctggctaca gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agcctggcat
    14761 atcagccgtc cgctggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg
    14821 agcggtttac tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc gctggaacat
    14881 gtgaccgctg cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg
    14941 caagtggtgg ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag
    15001 ctctgaaata tctcatttcg gcccacaacg acctgtgggc cgatggatat tactttaagc
    15061 cttccgctga cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgctg
    15121 caatgtgctc taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa
    15181 acagataggc atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg
    15241 cctcgttcac atattgcagc ttcttctcca gctgcgcgcg aactgtcggt ttgtactctt
    15301 ccggtgtatc agggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat
    15361 tcagccattc gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt
    15421 cggggacgct gtcggcaaga tactgcataa tcgctacgcc ttccgtcagc aaagtaccgt
    15481 catccagcag caatgcaggc acctgcccct tagggttaac gacaaagtaa tcgtcaccgt
    15541 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
    15601 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
    15661 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct
    15721 aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctaca gcagctttcg
    15781 ccgctttgtc tgcagcgtca tcctgcgtca tacggtgcag tttaggcgcg gtcagcagca
    15841 tcagtactgc aataatggca gtagcgacac caatctgcaa gaatacgcga ccataaactt
    15901 ccagtgacat cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac
    15961 caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca
    16021 gacgctgcgg aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc
    16081 cgatgctctg caggccatag cttgcgacca gccagcttac agacacgata ccagcgtcag
    16141 acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga
    16201 ttgcaaactt ggttggcatc ggcagggtat cgcccatctt gttatagata gcggccagaa
    16261 tcggactacc gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg
    16321 ccagacccag aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca
    16381 tctggctgta cagcacgaag aagataatgg cttcgagcat caggatgaag gcaacgatca
    16441 ttttacgacg cgcagcacct ttcatcgcga aggcttcttt accgaagata accacgatac
    16501 cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagc agccaggtgg
    16561 cgatagcgat cagtgccaca acaccaataa tggtcagcag caggttacgg tagttgatag
    16621 gctcgaagtc tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa
    16681 cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg
    16741 cggccagcca cggcgtagca atcatagaga agaaagagcc gatgttgacg gacatgtagt
    16801 acatggtgaa tgcaccgtcc agacgcgggt cgtttttctc atagcatgta gaaagcagag
    16861 aagacgggtt agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa
    16921 cgataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag
    16981 cgccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag
    17041 cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag
    17101 acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa
    17161 aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt
    17221 tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta
    17281 catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt
    17341 tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt
    17401 tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc
    17461 cagcgttaaa tttttcaaaa aacgtaacgg cagatttttt cactgcactg agaggggggt
    17521 gatggcttaa cggcgatatg ttctgctatt tagcgttttt actagtgaga tagtccagtt
    17581 tctgaaaaat agccagtgta atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa
    17641 atgattttaa cctgattctt gcaatgtttt tacattgcga cagcatcgtg atcttgatca
    17701 cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc
    17761 aaacggatta cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt
    17821 aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg
    17881 tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg
    17941 cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac
    18001 tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca
    18061 aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg
    18121 aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc
    18181 gcagcacgat cttccggaac ctcgccatta tgctgctcca gtaagatacg gcaggttttg
    18241 atgatatttt ctgctttgct gttataaagc ccaatcgttt tgatataggt tttcacccct
    18301 tcaacgccca gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc
    18361 gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca
    18421 aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg
    18481 aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg
    18541 cacgttctgc agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc
    18601 ctgccagcat cagtcccagg ccaataaatg cacctggagg cagcatcgcc agcaggaaag
    18661 gggagtcggt gtggaaaatt tccacgcgta atacttttgc ccagctacct aacagcgcat
    18721 ctgcaccgtc aaacaatgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca
    18781 tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc gccggacctt
    18841 ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa
    18901 taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag
    18961 cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc
    19021 agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc
    19081 ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagaggacaa aggccgagca
    19141 actggaccag cgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc
    19201 tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt
    19261 acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga
    19321 actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatct gcaccactga
    19381 tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt ttatcgccaa
    19441 gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgccg ttaaaatcgg
    19501 ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg
    19561 cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact
    19621 ctggcgcagt taccagatag caactctgtg ccagcgcatt gttatagcgt tcggctggca
    19681 gcacctgatc aaataatgcc ttttgttgca gactggcctg ttcagcaatc gtcgttttgg
    19741 tcatctggtt gatggccgca gttaaccctg ttgaacccgc tgcaaatagc gctaacgtaa
    19801 tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg
    19861 gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca
    19921 cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc gcaagcgcgc
    19981 cgaaaataag acgaccacga ttggtcgtag aagcggtaac cgggtcagtc aaaataaaga
    20041 atgcgccgag catggtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg
    20101 gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa
    20161 tatgccagcg aatcgctttc tgccatagca accacaggcc gccagccagc caggcgagat
    20221 ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct
    20281 gttcaaccga atgaccggca cggacagagg ttttaaatgt atccagcggt gtcgcctgac
    20341 taatgccatc aatacctaag cgtagtgtgt tcatatcacc accactggcg gtatgcccgc
    20401 tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg
    20461 gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg
    20521 ctggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa
    20581 acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt accgccagca
    20641 ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta
    20701 gtttgagtac gagagcttcg gctaacagag cactaaccga tgccaacagg atctgaacga
    20761 gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct gcgagcaaca
    20821 ccaacagcat aatgcgcgat gtctggcgct ggttatggat ataaggggag ctagctattc
    20881 tgaataccat ttagtcctcg tttacaacct tctgctgggc ggcttttttg gcctgagcgc
    20941 gggcaatagc cgcggcaact gccgctttgc gcggatcaac ctgttcttct ggttctgcgt
    21001 tagtctgttg ctgttccagc
//
LOCUS       DAEVZA010000090        20799 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEVZA010000090 DAEVZA010000000
VERSION     DAEVZA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.090"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            228..1037
                     /gene="pppA"
                     /locus_tag="LAF92_003318"
     CDS             228..1037
                     /gene="pppA"
                     /locus_tag="LAF92_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HBI9792274.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            1103..1513
                     /gene="gspS2"
                     /locus_tag="LAF92_003319"
     CDS             1103..1513
                     /gene="gspS2"
                     /locus_tag="LAF92_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HBI9792275.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAKNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            1531..2490
                     /gene="gspC"
                     /locus_tag="LAF92_003320"
     CDS             1531..2490
                     /gene="gspC"
                     /locus_tag="LAF92_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HBI9792276.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTNKKAVSDEAKQAVAEPAASAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            2520..4580
                     /gene="gspD"
                     /locus_tag="LAF92_003321"
     CDS             2520..4580
                     /gene="gspD"
                     /locus_tag="LAF92_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HBI9792277.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            4580..6073
                     /gene="gspE"
                     /locus_tag="LAF92_003322"
     CDS             4580..6073
                     /gene="gspE"
                     /locus_tag="LAF92_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HBI9792278.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            6073..7296
                     /gene="gspF"
                     /locus_tag="LAF92_003323"
     CDS             6073..7296
                     /gene="gspF"
                     /locus_tag="LAF92_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBI9792279.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDQTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            7313..7768
                     /gene="gspG"
                     /locus_tag="LAF92_003324"
     CDS             7313..7768
                     /gene="gspG"
                     /locus_tag="LAF92_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HBI9792280.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            7772..8335
                     /gene="gspH"
                     /locus_tag="LAF92_003325"
     CDS             7772..8335
                     /gene="gspH"
                     /locus_tag="LAF92_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HBI9792281.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDAPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            8332..8703
                     /gene="gspI"
                     /locus_tag="LAF92_003326"
     CDS             8332..8703
                     /gene="gspI"
                     /locus_tag="LAF92_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HBI9792282.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            8700..9299
                     /gene="gspJ"
                     /locus_tag="LAF92_003327"
     CDS             8700..9299
                     /gene="gspJ"
                     /locus_tag="LAF92_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HBI9792283.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            9302..10279
                     /gene="gspK"
                     /locus_tag="LAF92_003328"
     CDS             9302..10279
                     /gene="gspK"
                     /locus_tag="LAF92_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBI9792284.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLEQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            10276..11454
                     /gene="gspL"
                     /locus_tag="LAF92_003329"
     CDS             10276..11454
                     /gene="gspL"
                     /locus_tag="LAF92_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBI9792285.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPVVARKQIPTILPALLEEELNQDIDELHFAPLKINQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMACDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            11456..11992
                     /gene="yghD"
                     /locus_tag="LAF92_003330"
     CDS             11456..11992
                     /gene="yghD"
                     /locus_tag="LAF92_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HBI9792286.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(12141..12216)
                     /locus_tag="LAF92_003331"
     tRNA            complement(12141..12216)
                     /locus_tag="LAF92_003331"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12181..12183),aa:Phe,seq:gaa)
     gene            complement(12322..13029)
                     /locus_tag="LAF92_003332"
     CDS             complement(12322..13029)
                     /locus_tag="LAF92_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBI9792287.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            13427..15562
                     /gene="speC"
                     /locus_tag="LAF92_003333"
     CDS             13427..15562
                     /gene="speC"
                     /locus_tag="LAF92_003333"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBI9792288.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQTRFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(15612..16868)
                     /gene="nupG"
                     /locus_tag="LAF92_003334"
     CDS             complement(15612..16868)
                     /gene="nupG"
                     /locus_tag="LAF92_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBI9792289.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(17070..18149)
                     /gene="mltC"
                     /locus_tag="LAF92_003335"
     CDS             complement(17070..18149)
                     /gene="mltC"
                     /locus_tag="LAF92_003335"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBI9792290.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(18214..18489)
                     /locus_tag="LAF92_003336"
     CDS             complement(18214..18489)
                     /locus_tag="LAF92_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBI9792291.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(18517..19569)
                     /gene="mutY"
                     /locus_tag="LAF92_003337"
     CDS             complement(18517..19569)
                     /gene="mutY"
                     /locus_tag="LAF92_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBI9792292.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            19730..20449
                     /gene="trmB"
                     /locus_tag="LAF92_003338"
     CDS             19730..20449
                     /gene="trmB"
                     /locus_tag="LAF92_003338"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBI9792293.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            20449..20775
                     /locus_tag="LAF92_003339"
     CDS             20449..20775
                     /locus_tag="LAF92_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBI9792294.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
BASE COUNT         4928 a         5249 c         5820 g         4802 t
ORIGIN      
        1 agagggaaag ctgcgtttat ttgcgttggc agacacctgt aggccggata aggcgctcgc
       61 gccgcatcca gcatgaaata aggcgcatcg ggttaacaca tatgcccgat gcgccatctt
      121 atcgggtctt cagggaaagc cgcgattact tgcgttggca gatacctgta ggccggataa
      181 ggcgttttac gccgcatccg gcaagttaca acaaacaacc tttaaccatg ctttttgatg
      241 tttttcagca ataccccgtg gcgatgccca tactggctac cgtcggagga ttaatcatcg
      301 gtagtttttt gaatgtggtg atttggcgtt accccatcat gctgcgccaa caaatggcgg
      361 agtttcatgg agaaatgccg agtgcgcagt caaaaataag ccttgcgcta ccgcgttcgc
      421 actgcccaca ttgtcagcag accatccgga tacgtgacaa tattccgctg ctctcctggt
      481 tgatgctcaa agggcgctgc cgcgactgtc aggcgaaaat cagcaagcgt tatccgctgg
      541 tggagttatt gacggcactc gcttttttgc tggcgagtct ggtctggccg gaaagtggat
      601 gggcgctggc ggtgatgata ttatccgcct ggctgattgc cgcgagcatc atcgacctcg
      661 atcaccaatg gctgcccgat gtttttactc agggcgtatt gtggacggga ctaattgcgg
      721 catgggcgca gcagagcccg ctcacgctac aagatgcagt caccggcgtc ctggtggggt
      781 ttatcacttt ttactctctg cgctggatag ccggaatagt tctgcgtaaa gaagcattag
      841 gcatgggcga tgtattattg ttcgctgcgt taggtagttg ggtggggccg ttgtcgctac
      901 ccaatgttgc tttaatcgcc tcatgctgcg gcctgatata tgccgttatt acaaaaagag
      961 gatcaaccac actgcctttt ggaccgtgtt taagtctggg cggtatagca acaatttatc
     1021 tacaggcatt gttttaatga taacaacgtc attatcaaag tgacatttta actcttatta
     1081 ataaccttag agattattta ccatgtcgat aaaacaaatg ccagggaggg tattaatatc
     1141 gctattgttg agcgttacag gattattaag tggctgtgcc agccataatg aaaatgccag
     1201 tttactggcg aaaaaacagg cgaaaaatat cagccaaaac ctgccgatta aatctgcggg
     1261 atatacctta gtgctggcac aaagtagcgg cacgacggta aaaatgacca ttatcagcga
     1321 agcggggact cagaccacgc agacgcctga cgccttttta accagctatc aacgacaaat
     1381 gtgcgctgac ccaacggtga aattaatgat taccgaggga attaattaca gcataacgat
     1441 taatgataca cgtacaggta accagtatca gcggaaactg gatcgtacca cctgtggaat
     1501 agtcaaagca taacgtcggg tagatataaa ttggcgcggg ttgtttttcg tgacgcacga
     1561 atttatctca ttcaatggct gacaaaaatt cgtcacactc ttaaccagag acaatctttt
     1621 aatacagaca aagagcatct gcgaaaaatt gtacgcggga tgttctggct gatgctgctt
     1681 attatttctg caaaagtggc gcattcactc tggcgctatt tctccttttc tgcggaatat
     1741 acggctgttt ccccatcggc gaataaaccg ccccgtgcgg atgcaaaaac gttcgataaa
     1801 aatgacgtgc aattaatcag ccagcaaaac tggtttggca aatatcagcc cgtcgccacg
     1861 ccggtaaaac aacccgaacc tgtgcctgtg gcagaaacgc gtcttaatgt ggtgctgcgc
     1921 gggattgcct ttggtgccag acccggcgcg gttattgaag aaggcggtaa acagcaggtc
     1981 tatttgcagg gtgaaacgct tggctcgcac aatgcggtga ttgaggaaat caatcgcgac
     2041 catgtgatgc tgcgctatca gggaaaaata gagcgcctga gcctggctga agaggagcgt
     2101 tccactgttg ccgtgaccaa caaaaaagct gtcagtgacg aagcaaagca agctgttgct
     2161 gagcctgctg ccagtgcgcc agttgagatc ccgactgccg tgcgtcaggc actggcgaaa
     2221 gatccgcaga aaatttttaa ctatatccag cttacgcctg tgcgtaagga ggggattgtc
     2281 ggttatgcag tgaagccggg ggcagatcgt tctctgttcg atgccagcgg tttcaaggaa
     2341 ggcgatatcg ccattgcgct aaatcagcag gatttcactg atccacgagc aatgattgct
     2401 ctgatgcggc agttaccttc aatggattcc attcaactta cggttttacg caagggtgcg
     2461 cgccacgaca tttccatcgc gctgcgctaa ccgcatttaa tccaggagaa tcattcatcg
     2521 tgttttggcg tgatattacg ttgtcggtct ggcgtaagaa gacaactggc ctcaaaacaa
     2581 aaaagcgttt actgccgctg gtgctggcag cggcattatg cagttcaccg gtctgggcgg
     2641 aagaagccac tttcaccgct aattttaaag ataccgacct gaaatcgttc atcgaaaccg
     2701 tcggcgctaa ccttaataaa accatcatta tggggccggg cgtacagggg aaagtgagta
     2761 ttcgcaccat gaccccgctc aatgaacgcc agtattacca gctattcctt aacctgttgg
     2821 aagcacaggg gtatgccgtc gtaccgatgg aaaacgacgt gctgaaggtg gtgaaatcca
     2881 gcgctgcgaa agtcgagccg ctgccgctgg tcggtgaagg cagcgacaac tacgcgggcg
     2941 atgaaatggt caccaaagta gtgccggtac gtaatgtgtc ggtacgcgag ctggcaccga
     3001 ttctgcgcca gatgatcgac agcgcaggct caggcaacgt tgttaattac gatccctcca
     3061 acgtgattat gctcaccgga cgcgcctccg ttgtggagcg gctgacggaa gtgatccagc
     3121 gtgtggatca cgcgggtaat cgcactgaag aggtgatccc gctggataac gcctcggctt
     3181 cggaaatcgc ccgcgtgctg gaaagcctga cgaaaaacag cggcgagaac cagcctgcga
     3241 cgctgaaatc tcaaattgtc gccgacgaac gcaccaacag tgtgattgtc agtggtgacc
     3301 cggccacgcg ggacaaaatg cgccgcctga tccgtcggct ggactcagaa atggaacgca
     3361 gcggcaacag ccaggtgttc tatctcaaat acagcaaagc cgaagatctg gtcgatgtgc
     3421 tgaagcaggt cagcggcacg ctcacggcgg ctaaagaaga ggcggaaggc acggtcggta
     3481 gcgggcgtga gattgtatcc atcgccgcca gcaaacacag taatgccctg attgtaaccg
     3541 caccgcagga catcatgcag tcgctgcaaa gcgtgattga acaactggat attcgccgtg
     3601 ctcaggtgca cgtcgaggcg ttgatcgtgg aagttgccga aggtagcaat atcaacttcg
     3661 gcgtgcagtg ggcgtcgaaa gatgccggat taatgcagtt tgctaatggt acgcagatcc
     3721 ctatcggtac gctgggggca gccatttctc aggcgaaacc gcagaaaggc tcgacggtga
     3781 tcagtgaaaa cggcgctacc accattaatc cggataccaa cggcgatctc tccacactcg
     3841 cccagcttct ttccggcttt agcggtacgg cggttggtgt ggtgaaaggc gactggatgg
     3901 cgctggtgca ggcggttaaa aacgactcca gctctaacgt gctctccacg ccgagcatca
     3961 ccacgctgga caaccaggaa gccttcttca tggtgggcca ggacgttccg gtattaaccg
     4021 gctctaccgt tggctccaat aacagcaatc ctttcaatac agtagaaagg aaaaaagtcg
     4081 gcatcatgct gaaagtcacg ccgcagatta acgaaggtaa cgcggtacag atggtgattg
     4141 agcaggaagt ctcgaaggtg gaaggacaga ccagcctcga cgtggtgttt ggtgagcgca
     4201 aactgaaaac caccgtgctg gctaacgatg gtgagctgat tgtgcttggc ggtctgatgg
     4261 acgaccaggc gggagaaagc gtggcgaaag tgccgctgct tggcgatatc ccattgattg
     4321 gtaacctgtt taaatcgacg gcggataaaa aagaaaaacg taacctgatg gtgtttatcc
     4381 gtccgaccat tctgcgtgac ggtatggcgg cagacggcgt gtcgcagcgc aaatataact
     4441 atatgcgcgc cgaacagatc tatcgtgatg agcaaggttt aagcctgatg ccgcacaccg
     4501 cgcagccggt actgccagcg caaaatcagg ctctaccgcc ggaagttcgt gcgttcctta
     4561 atgccgggag aacgcgttaa tggtgcctgt agcacaggaa accaccgcta acaccgtgcg
     4621 tctgccctac agtttcagcc gtcggtttag cctggtggca tggtgcgaag cgtcgctgga
     4681 gatcctccat gttcatccgt tgtcgctctc tgttttgcag gagctgcagc gggggctgaa
     4741 cgcgcccttt acgctgcggc aaatcgacga ggccgaattt gaacagcggc tgaatgcggt
     4801 ctggcagcgg gactcttccg aggctcgcca gctgatggaa gatctcggtt ctgccgagga
     4861 cttttttacc ctcgcggaag aactgccgga aacggaagat ctgctggaaa gcgacgacga
     4921 tgcgccgatc atcaaactga tcaacgccat gctggcagag gcaatcaaag aaggcgcttc
     4981 ggatatccac attgagacgt ttgaaaagag tctggtgatc cgttttcgtg ttgacggcac
     5041 attacatgaa atgctgcgtc cggggcgcaa actggcctcg ctgctggtgt cgcgtatcaa
     5101 ggtgatggcg cggctggaca ttgccgaaaa gcgcgtgccg caggatggac gtattgcgct
     5161 gttgctgggc ggccgggcga ttgacgtgcg tgtctccacc atgccttctg cctggggcga
     5221 gcgcgtggtg ctgcgactgc tggacaaaaa ccaggcccgc ctgacgctgg agcgtctggg
     5281 gcttagccag caactgaccg cgcagttgcg ccagctgtta cacaaaccgc acggcatctt
     5341 tctggtgacg gggccgacgg gttccggcaa aagcaccacg ctgtacgccg gattgcagga
     5401 gctgaacaac cattcgcgca acattctcac ggttgaagat cccatcgaat acatgattga
     5461 agggatcggt cagacgcagg ttaacacccg cgtcggcatg acctttgccc gtgggctgcg
     5521 cgcgattttg cgtcaggacc cggatgtggt gatggtcggt gaaatccgcg ataccgaaac
     5581 cgcagaaatc gccgtccagg cttcacttac cggacacctg gtcctttcca cgctgcatac
     5641 caacacagcg gtgggggcga tcacacgttt gcaggatatg ggcgtggagc ctttcctgct
     5701 ctcttccagt ctgacgggcg tgatggcgca gcgactggtt cgcacgctgt gtcccgactg
     5761 ccgccagccc gcaccagcca ctgacgaaga aaaacgcctg ctgggaatta ccgacgcccg
     5821 taccgtcact ctgtaccatc cacagggctg tcccgcctgt aatcacaaag gttttcgcgg
     5881 acggactgcc atccatgagc tgatcgtggt ggatgccaca ttgcgtgatt tgatccaccg
     5941 tcaggccggg gagctggagc tggaacgtta tgtccgacaa cactctgcgg gtatccgcag
     6001 caacggcatt gagaaagtgc tcgccggaga aacctctctc gatgaagttc tgcgggtaac
     6061 catggaggcg taatggcact gttttactat caggcgctgg agcgtaatgg tcgcaaaacc
     6121 aaaggtatga ttgaggcgga ttccgcgcgt catgcccgcc agttgttgcg cggtaaagag
     6181 cttatccccg tgcatattga agcccggatg aatacttcgt caggggggat gttgcagcgt
     6241 cggcggcacg cacatcgtcg cgtggcggcg gcagatcttg cgctgttcac gcgccaactg
     6301 gcaacgctgg tacaggcagc aatgccgctg gaaacctgct tacaggcggt cagtgagcaa
     6361 agtgaaaaac tgcatgtaaa aagcctcgga atggcgctgc gcagccggat tcaggaaggt
     6421 tacaccctgt cggacagcct gcgcgaacat ccccgcgtct ttgattctct gttttgttcg
     6481 atggttgctg ccggagaaaa atccgggcat ctcgacgtgg tgcttaatcg cctggcagat
     6541 tacaccgaac agcgacagcg cctgaaatca cgtctattgc aggccatgct ctatccgctg
     6601 gttctgctgg tggtggcaac gggcgtagtc actattttgc tgacggcagt ggtgccgaaa
     6661 atcatcgaac agtttgatca tctcggacac gcgctacccg cctcaacccg cacgcttatt
     6721 gccatgagcg acgcgttaca ggccagcggc gtttactggc tggcgggttt gctggggctt
     6781 ctggtgctgg ggcaacggct acttaaaaat cctgctatgc gcctgcgctg ggatcaaacc
     6841 gtgctgcgcc tgcctgtgac ggggcgtgtt gcgcgcgggc tgaatacggc gcgtttttcc
     6901 cgcaccttaa gcatcctcac cgccagcagt gttccgctac tggaaggtat tcagaccgct
     6961 gccgctgtgt cggcaaatcg ctatgtcgaa caacaactac tgctggcggc agatcgcgtc
     7021 cgcgaaggaa gcagtctgcg cgccgcgctg gcggagttgc gcctgttccc cccgatgatg
     7081 ctgtacatga tcgcctccgg cgaacagagc ggcgaactgg aaaccatgct tgagcaggcc
     7141 gctgttaacc aggaacggga atttgatacc caggtggggc tggcgttggg gctgtttgag
     7201 cctgcactgg tggtgatgat ggcgggcgtg gtgctgttta ttgtcatcgc catcctcgag
     7261 ccgatgctac aactgaataa tatggttgga atgtaattta cggagttatc acatgaattc
     7321 gttatcccgc acacaaaaac cacgggcagg ttttaccctg ctggaagtga tggtggtgat
     7381 tgttattctt ggcgtcctgg caagtctggt ggtgcctaac ctgttgggca acaaagagaa
     7441 agccgatcgg caaaaagcca tcagcgatat cgtggcgctg gaaaacgcac tggatatgta
     7501 ccgactggat aacgggcgtt atccgaccac agaacagggg cttgaggcgc tgatccagca
     7561 gccagccaat atggcggact cccgcaacta ccgtactggt ggatacatta aacgactgcc
     7621 aaaggatccg tggggcaatg attatcagta tctcagtccg ggtgaaaaag ggctgtttga
     7681 tgtttatacc ctgggggcgg atggtcagga aaacggggaa ggcgctggcg cagatatcgg
     7741 taactggaat ttgcaggagt ttcagtaagc agtgcctgaa cgcggattca cacttctgga
     7801 aatcatgctg gtgatttttc ttatcggcct tgccagtgcg ggcgtggtac agacttttgc
     7861 gaccgattct gaatcgcccg cgaaaaaagc ggcgcaggat tttctgactc gctttgcgca
     7921 gtttaaggac agggcagtga tcgaagggaa aacactcggt gtgctaatcg acgcgcctgg
     7981 ctatcagttt atgcagcgtc gtcaggggca gtggttgccc gtttccgcga cccggttatc
     8041 ggcacaggtt acggtgccaa aacaggtgca gatgctgtta caacccggaa gtgatatctg
     8101 gcagaaggag tatgcgctgg agctgcaacg tcgtcgcctg acgctgcacg atattgaact
     8161 ggagctgcaa aaagaggcga aaaagaagac gccgcagatc cgtttttcgc cttttgaacc
     8221 cgccacgccg tttacgctgc gcttctactc ggcggcgcaa aacgcatgtt gggcggtaaa
     8281 gctggcacac gatggcgcgt tatccctcaa tcaatgtgat gagaggatgc catgaagcgc
     8341 ggatttacct tgctggaagt gatgctcgcg ctggcgattt ttgcgctggc tgccacggcg
     8401 gtgttacaga ttgccagcgg tgcgctgagt aatcagcaaa ttcttgagga aaaaacggta
     8461 gcgggctggg tggctgaaaa ccagaccgcg ctgctctacc tgatgacccg cgagcagcgg
     8521 gcggtcaggc agcagggcga gagcgatatg gcaggaagcc gctggtactg gcgaaccaca
     8581 ccgctgagta caggcaacgc gctgctccag gcggtggata ttgaagtcag ccttcacgaa
     8641 gacttttcgt cggtgattca gtcacgacgc gcctggttta gcgccgtggg aggccaacag
     8701 tgagaaggac tcgcgctggt ttcacgttac tggaaatgtt ggtggcaatt gccatttttg
     8761 cctcgctggc actgatggcg cagcaggtga ctaacggcgt cacgcgcgtg aatagcgccg
     8821 tcgccggaca cgatcaaaaa ctaaacctca tgcagcaaac gatgagtttt ctgacccacg
     8881 atctgaccca aatgatgccg cgtccggtaa gaggcgatca gggccagcgt gaaccagcgt
     8941 tactggctgg cgctggcgtg ctggcgtctg agagtgaagg aatgcgtttt gtgcgcggcg
     9001 gtgtggttaa cccgttgatg cgtctgccgc gcagtaatct gctcaccgtc ggttaccgca
     9061 ttcatgacgg ttatctcgaa cggttagcct ggccgctgac cgatgccgca ggcagcgtga
     9121 agccgacaat gcaaaagttg attccggccg attcgctccg tttgcagttc tacgacggca
     9181 cgcgctggca ggagagctgg tcgtcagtac aggcgatccc tgtggcggta cgcatgaccc
     9241 tgcattcgcc gcaatggggc gagattgaac gcatctggtt gttacgcggg ccgcaataat
     9301 catgatcacc tcaccaccaa aacgcggaat ggcactggtc gtggtgctgg tattgctggc
     9361 agttatgatg ctggtaacca tcacgctttc cgggcggatg cagcagcaac tgggaagaac
     9421 gcgcagccag caggagtacc agcaggcgct gtggtacagc gccagtgcag aaagcctggc
     9481 gctgagcgcg ctcagtctga gcctgaaaaa tgaaaagcgc gtgcatctgg aacagccgtg
     9541 ggcttccggc cctcgttttt tcccactgcc gcaggggcaa atcgccgtca ctctgcgtga
     9601 cgcacaggcc tgctttaacc tgaatgccct cgctcagccc acaacggcgt cgcgtccgct
     9661 cgcggtacaa caactgattg ccctgatcac gcgcctggat gtgcctgctt atcgggccga
     9721 actgatagcc gaaagcctgt gggagtttat tgatgaagac cgcagcgtgc agacgcgtct
     9781 gggccgtgaa gacagcgaat atctcgcccg ctcggtgcct ttctacgccg ccaatcaacc
     9841 gctggccgat atcagcgaga tgcgcgtggt gcagggaatg gacgccgggc tttatcaaaa
     9901 actgaaaccg ctggtctgtg cgctgccgat gacccgccag caaatcaaca tcaatacttt
     9961 agacgtcacg caaagtgtga ttcttgaggc gctgtttgac ccgtggttaa gccctgttca
    10021 ggcgcgggcg ttattacaac aacgtccggc gaagggctgg gaagatgtcg atcagtttct
    10081 tgcacagccg ctacttgctg acgtcgatga gcgtactaaa aaacagctaa aaaccgtcct
    10141 gagcgtggac agcaattact tctggctgcg ttcagatatc accgtgaatg agattgaact
    10201 gacgatgaac tcgttaattg tccgcatggg cccacaacac ttttcggttc tctggcatca
    10261 gacaggagaa agtgagtgag ttccatcctt gagatttttt tcccgctttg cgccgctgat
    10321 cccatccatt ggcagcgccg tacacccgat gtggagcacg gtatctggtc tgacgttgct
    10381 aacgaacagc tccagcaatg gctgcaaact gatgcgattc gactctacat tcccggcgaa
    10441 tggatcagcg tctggcaggt tgaactgcct gttgtcgccc gcaagcagat accgactatt
    10501 ctgcccgcct tactggaaga agaactgaac caggatatcg atgaactgca tttcgccccg
    10561 ttgaaaatca atcagcaact ggcaaccgta gcagtgattc accaacagca tatgcgcaac
    10621 attgcgcagt ggttgcagga aaacggcatc acccgcgcta ccgtcgcgcc ggactggatg
    10681 tccattcctt gtgggtttat ggcttgcgat gcgcaacggg ttatctgccg catcgatgaa
    10741 tgccggggat ggagcgccgg gctggcgctg gctccggtca tgttccgcgc ccagctcaat
    10801 gagcaggatt taccgctttc gctaaccgta gtcggcattg caccggaaaa gctatcagca
    10861 tgggctggcg cagacgctga acgcctgacc gttacagctc tgcccgccat taccacttat
    10921 ggcgaaccgg aagggaacct gctgacaggg ccgtggcagc ctcgcgtcag ctaccgaaaa
    10981 cagtgggcgc gctggcgggt gatgattctg ccgatattgc tgattctggt tgcgctggca
    11041 gtggagcggg gcgtgacgtt atggagcgtc agcgaacagg tggcgcaaag ccgcacccag
    11101 gcggaggaac agttcttaac gttgttcccg gagcagaagc ggattgtgaa tttacgctct
    11161 caggtgacga tggcgctgaa aaaatatcgc ccacaggccg acgatacccg gctgctcgcc
    11221 gagttgtcag cgatagccag caccctgaaa tcagcgtcac tttccgacat cgaaatgcgt
    11281 ggtttcacct ttgatcaaaa acgccagacg cttcacctcc agttgcgggc tgctaacttt
    11341 gccagtttcg acaaactgcg tagtgcactg gcaaccgatt atgttgtgca acaggacgca
    11401 ttacagaaag agggtgatgc ggtttccggc ggcgtaacgt tgcggaggaa ataacatgtt
    11461 acgcgataaa tttattcact attttcagca atggcgtgaa cgccagttaa gccgtggcga
    11521 acactggctg gcacaacacc tggcggggcg ttctccgcgt gaaaagggca tgttactggc
    11581 agcggtggtg ttcctgttta gcgtcggata ttacgtcctc atctggcagc cgttgagcga
    11641 acggatagaa caacaggaga caatgttgca gcaactggtg gcgatgaaca ccagactgag
    11701 gagcgccgcg ccggatatta tggcggcgcg aaaatccgcc acaacaacgc ctgcgcaggt
    11761 atcgcgggtt atcagtgaca gcgcttccgc gcactcggta gtcatcaagc ggatagccga
    11821 gcgtggggag aatattcagg tctggataga tccggtggtg tttaatgacc tgctgaactg
    11881 gttaaacgcg ctggatgaaa agtatgcgct gcgggtgaca caaattgatg ccagtgctgc
    11941 tgagaagact gggatggtga atgtgcagcg gctggagttt ggacgggggt aaagtatcag
    12001 gagattagta tcaataaaat tactctcgtt aacgtggcga gagtaattcc cccagacttt
    12061 ataagcaaac ttttttcgtg aaggttattg tcagatccaa aagcaaaaac ccgccttgtg
    12121 ggcgggttct taagaattag tggtgcccgg actcggaatc gaaccaagga cacggggatt
    12181 ttcaatcccc tgctctaccg actgagctat ccgggcaacg gggcgcatta aacctgattc
    12241 gcctcgtctc gtcaatcaaa tttcttcaat ttactgcaga ctgcacaatc tatcatcact
    12301 ttgccgctat tgcacgcatt gtcaggcaaa ccaggcggtc caggcggcgg aaagcggcat
    12361 tgccagaagg agtgctggaa gcatgttgac caccgggaac attttaatgc cacagatgcg
    12421 taatccggtt gccagcagca ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg
    12481 tgtggtcagc ggtaatatca gcgcggcagc ccacgccagc gttaactgga tgatcagtaa
    12541 tgggatacta attaccgaca ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa
    12601 gaaatcaaga aatgacttgg cgattaaaat actcggatcg ccggtcatcc cttcgttcat
    12661 cgccccgaag atcccggtgc cgctggcgca aaacaggaca ataatcgcga cataattctg
    12721 aataaaagat tcatgcgctg gcttcttacg tgagtgacga aacagatttt gtgctttggc
    12781 gactgctgta ttgacacctt tctccagcaa acaaatttcg ccaattagag cgccaagtag
    12841 cgtagctaaa accatcgctg gaaggttggc gcatttcaca accagtaaaa taccaatccc
    12901 cagcgatgcc agaccaaaaa tagatgtcat ggagacgcgg atacgttccg gtaagcgttg
    12961 gctgagcagt gcgccgagaa cgccacccag taagactgca ctagcgttga taaaagggcc
    13021 gatgaccaca agagttcctg ttagctgtat tgtttgattt tcttattatg aactttttgc
    13081 cttgttgatg gcttgtctgg tcgcaaagtc ctggcttgca cgctttagcg aaaggtgcca
    13141 tgattgcgcg aattttctcc tctctgtacg gagtttgccc gatgcacgcc atctccttac
    13201 attctctcgc ttatcgccgt ttcgcgcgaa acgtttccct ttctatgtta ctgctcatgc
    13261 ggtgaagtta acgcacgctc actgcaggac aacagtaaaa tcagagcgtt tctgctttta
    13321 ctgatgtctg gcggtcggag ctggtgacca gtttgaccca tatctcatgg ggcagggttt
    13381 tccaccttgt ccggtattct tacttccccg aaacgggttt gcgcttatga aatcaatgaa
    13441 tattgccgcc agtagtgaac tggtatcccg actttcttct catcgtcgcg tggtggcgtt
    13501 gggagatact gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag
    13561 tggcattctt gcgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga
    13621 acatgctgtt gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcagtg
    13681 gctggagctg gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga
    13741 cacgctgacg cagtacgttg agatgggcaa cagcaccttt gcttgccctg gacatcaaca
    13801 tggtgcgttt tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa
    13861 cgtctttcgc gccgatatgt gtaacgctga cgtaaaattg ggcgatctgc ttattcatga
    13921 aggatcggcg aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta
    13981 ttttgtgctg aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tgttaacgcg
    14041 tggcgatctg gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgttgat
    14101 tcaggcgggg gcgacgccgg tctatctgga agcttcacgt aacccgtttg gtttcattgg
    14161 cggtattgat gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc
    14221 gccagaaaaa gccgacctgc cgcgcccgtt tcgcctggcg attatccagc tgggaaccta
    14281 tgacggcact gtctacaacg cccgtcaggt gatcgatacc gttgggcatc tgtgtgatta
    14341 cattctgttt gattccgcgt gggtaggtta tgaacaattt atcccgatga tggcggatag
    14401 ctcgccgctg ctattagaac ttaacgaaaa cgatccgggg atctttgtga ctcagtcggt
    14461 gcacaaacag caggcgggat tctcacagac gtcgcagatc cataaaaaag ataaccatat
    14521 ccgcggacag acgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc
    14581 ttctaccagc cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga
    14641 aggggagagt gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa
    14701 ggctattctt gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa
    14761 attgtggcag gattatccga cgtctgtgtt agccagcgac cgccgttttt tcagttttga
    14821 gccgggggcg aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc
    14881 gtgcaagctg ttactcacta caccaggtat caatgccgaa accggcgaat atagcgactt
    14941 tggcgttccg gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa
    15001 gtgcgatctc aactccattc tgttcttatt aactccggcg gaaagccacg agaagctggc
    15061 acaactggta gagatgttgg cgcagtttga acagcatatt gaggatgact cgccgctggc
    15121 tgaggtgttg ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg
    15181 ccagttgtgt caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa
    15241 agcgatgttc cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag
    15301 cgcttatatt cgcggtgaag tggagttggt gcggattcgt gatgccgaag ggcgaattgc
    15361 ggcagaaggg gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg
    15421 gggtggggcg gttcaacgtt atttccttgc actggaagaa ggggtgaatt tgttgccggg
    15481 attttcgccg gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt
    15541 gtacggttat gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt
    15601 tctttgcgta attagtggct aaccgtctgt gtgcctgtcg ggacacgaac gtgtttatat
    15661 ttgaacatcg ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat
    15721 acggtctgcc agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg
    15781 atgccgccga ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg
    15841 gcgcgaattg ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag
    15901 tcgaatgcgc aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc
    15961 gggtcgccgt aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt
    16021 acgttcttaa taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca
    16081 gagatctgcg aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac
    16141 atcggatctt tgtcgaagct gtgcaggaag gtattaccga acatgttggt aatctgcagt
    16201 tccgcgccca gcagcattga gaagataaag aagattgcca tacgcttgtt tttaaacagc
    16261 gcgaatgcat cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc
    16321 ggaatatgcg gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata
    16381 tacagctgca tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata
    16441 aagccgatgg tgccccagat acggattggc gggaagtcag taacgatatc catcccggca
    16501 ttttgcaggc gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac
    16561 gagttaatca atatcacaag gaacatcgcc tccggtgtcg tgacctgtgc cgccatgaac
    16621 agcgtgatag cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg
    16681 tcggccacaa tccccagcag cgcaggcata aataccgcgg cgatacccag tgagctataa
    16741 actgcgccaa tagaagcacc gtcaaacttc agggtaacaa acatatagga gccgagggtc
    16801 gtcagccaac ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta
    16861 agattcatgt taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga
    16921 ccgtgcgaga tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa
    16981 ataattgcaa acattcccaa tttgcaaaac gaaattgtga tgtggataac atttttgccc
    17041 ctgagcatcg tcaggggcgg ttaatggaat tatcggcggc ggtaggattt ttgcgcggta
    17101 ttcactttat aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtctga
    17161 taaacatcgc ccggcgtcat ggtgttaata atattggcag cctgaatctt atcattcgaa
    17221 aagactcgca gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgc
    17281 gatgttgggt tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg
    17341 ccggtatcaa tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc
    17401 ccctgcgagc ggaacacatc tttcccggca gtatgttgta ccacctgcat taatcccagc
    17461 gcatcggaac ggctgaccgc atacgggtta aaggaagatt cggtctgcat aattgccaga
    17521 atcagcgact catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg
    17581 tgcgcacgtt tatcaaggtg gttcggtacc atgttaatgg tgacgctgta gatgatacgc
    17641 agcccgttac tgcggctttg cagacggttt ttcagcagat aatccgcgaa gttgcttgcg
    17701 cgaccttccc agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt
    17761 tctttcgaaa tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca
    17821 cccatcagca acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgtcccggcg
    17881 atagtttcga tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga
    17941 tcggtgtatt tcacgtagtc cttaggaccg gcgatcacca cctctttgaa tccccagatg
    18001 ttctcaatat tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt atctttgacc
    18061 caggcttcgt tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca
    18121 atcaaagcca gcgcgagata ttttttcatc attccgggtg cgtgttgtgt ttgtttttat
    18181 gcaagttgcc ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc
    18241 ctcgatatgc acctctttac cctcgaacag gaagttgacc atctcctgct caagcagctt
    18301 gcggtgctcg gcattcatca tgttgagttt cttttcatta atcagcatgg tttgcttgtg
    18361 ctgccactgc gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagctcgcc
    18421 ggggtacagc tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt
    18481 tctgctcata aatcatcctc tttatcgact cacgcgctaa accggcgcgc cagtgcgtaa
    18541 ctgctgtaac aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt
    18601 ataccagagc gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat
    18661 aggcacaata tctaagtgga aatggctgaa ggtatggcga aacgcggtca gctgcgtcag
    18721 gttatcggca gcaatctgcc gttgcgccag ccaatgccgc aaactttctt cgtcggcaaa
    18781 ctgcgggaaa cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac
    18841 ttcatcttcg tgctgtaata gcaaaaagta gccggtgcgc tccggcagcg tctgtttcgg
    18901 ttttttgccc ggataaagcg accagctatt gttggcggcg gcaatacatc cgttttgtag
    18961 cggacagagc gaacatttcg gtttcgagcg cgtacaaatc atcgcgccca aatccatcat
    19021 cgcctgatta aaccgttcca cgccgaccgc gggcgtcacc tgctcgctca aactccataa
    19081 tttattctcg acctcttttt tccctggcca gccgcttaca gcatagcagc gcgccagcac
    19141 ccgtttgacg ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc
    19201 acctgcggtg gaacgcccga cgcccggcag tgctgcaact tcctcaaagg tttccgggaa
    19261 tttaccgccg tgtaaggtcg ccacttgttg tgccgcttta tgcagattgc gcgcgcgggc
    19321 gtaatagcca agcccggtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc
    19381 ggtcaccgtc gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg
    19441 agtttgttgc aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca
    19501 gggcagagtt tttcgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga
    19561 cgcttgcatg gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca
    19621 gggtgtaaac cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac
    19681 tttggataat gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga
    19741 cgtcatttca ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt
    19801 gcgccgccag gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt
    19861 gatgggcgtt gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc
    19921 gccggtgacg cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga
    19981 tcgccccgag caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct
    20041 ggcttctgcg catgaagagg gcttaagcaa cctgcgcgtg atgtgtcacg atgcggttga
    20101 agtgctgcat aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga
    20161 cccgtggcac aaagcgcgcc ataataaacg ccgtatcgtt caggtgccgt ttgccgaact
    20221 ggtaaaaagc aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta
    20281 tgcggaacat atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag
    20341 caatgattac gtaccgcgtc cggcatcacg tccagtgacg aaatttgaac aacgtggtca
    20401 tcgtcttggt cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac
    20461 cgtagccgtc gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg
    20521 gtggcatggc gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat
    20581 tttattaacg aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc
    20641 tgggaaggtc tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt
    20701 gtgcgtaagt ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac
    20761 gtttggtggg actaagaaag catacgggcg atgacaaat
//
LOCUS       DAEVZA010000091        20607 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEVZA010000091 DAEVZA010000000
VERSION     DAEVZA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.091"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..574
                     /locus_tag="LAF92_003340"
     CDS             <1..574
                     /locus_tag="LAF92_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI9792295.1"
                     /translation="INIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSRD
                     RTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTMI
                     IAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEKL
                     RIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            666..938
                     /locus_tag="LAF92_003341"
     CDS             666..938
                     /locus_tag="LAF92_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBI9792296.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(994..1257)
                     /gene="yhcN"
                     /locus_tag="LAF92_003342"
     CDS             complement(994..1257)
                     /gene="yhcN"
                     /locus_tag="LAF92_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBI9792297.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(1622..2092)
                     /gene="argR"
                     /locus_tag="LAF92_003343"
     CDS             complement(1622..2092)
                     /gene="argR"
                     /locus_tag="LAF92_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBI9792298.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            2527..3465
                     /gene="mdh"
                     /locus_tag="LAF92_003344"
     CDS             2527..3465
                     /gene="mdh"
                     /locus_tag="LAF92_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBI9792299.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVSKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            complement(3528..4595)
                     /gene="degS"
                     /locus_tag="LAF92_003345"
     CDS             complement(3528..4595)
                     /gene="degS"
                     /locus_tag="LAF92_003345"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBI9792300.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(4685..6052)
                     /gene="degQ"
                     /locus_tag="LAF92_003346"
     CDS             complement(4685..6052)
                     /gene="degQ"
                     /locus_tag="LAF92_003346"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBI9792301.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(6206..6604)
                     /locus_tag="LAF92_003347"
     CDS             complement(6206..6604)
                     /locus_tag="LAF92_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBI9792302.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6798..7925
                     /gene="zapE"
                     /locus_tag="LAF92_003348"
     CDS             6798..7925
                     /gene="zapE"
                     /locus_tag="LAF92_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBI9792303.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            8144..8572
                     /gene="rplM"
                     /locus_tag="LAF92_003349"
     CDS             8144..8572
                     /gene="rplM"
                     /locus_tag="LAF92_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBI9792304.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            8588..8980
                     /gene="rpsI"
                     /locus_tag="LAF92_003350"
     CDS             8588..8980
                     /gene="rpsI"
                     /locus_tag="LAF92_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBI9792305.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            9375..10013
                     /gene="sspA"
                     /locus_tag="LAF92_003351"
     CDS             9375..10013
                     /gene="sspA"
                     /locus_tag="LAF92_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBI9792306.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            10019..10516
                     /gene="sspB"
                     /locus_tag="LAF92_003352"
     CDS             10019..10516
                     /gene="sspB"
                     /locus_tag="LAF92_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBI9792307.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(10559..11926)
                     /gene="dcuC"
                     /locus_tag="LAF92_003353"
     CDS             complement(10559..11926)
                     /gene="dcuC"
                     /locus_tag="LAF92_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBI9792308.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGISPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            12306..13097
                     /gene="nanR"
                     /locus_tag="LAF92_003354"
     CDS             12306..13097
                     /gene="nanR"
                     /locus_tag="LAF92_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBI9792309.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            13219..14112
                     /gene="nanA"
                     /locus_tag="LAF92_003355"
     CDS             13219..14112
                     /gene="nanA"
                     /locus_tag="LAF92_003355"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBI9792310.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            14221..15711
                     /gene="nanT"
                     /locus_tag="LAF92_003356"
     CDS             14221..15711
                     /gene="nanT"
                     /locus_tag="LAF92_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBI9792311.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            15759..16448
                     /locus_tag="LAF92_003357"
     CDS             15759..16448
                     /locus_tag="LAF92_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBI9792312.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            16445..17320
                     /gene="nanK"
                     /locus_tag="LAF92_003358"
     CDS             16445..17320
                     /gene="nanK"
                     /locus_tag="LAF92_003358"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBI9792313.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHLSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            17317..17781
                     /locus_tag="LAF92_003359"
     CDS             17317..17781
                     /locus_tag="LAF92_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBI9792314.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(17857..18162)
                     /locus_tag="LAF92_003360"
     CDS             complement(17857..18162)
                     /locus_tag="LAF92_003360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI9792315.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(18179..18658)
                     /locus_tag="LAF92_003361"
     CDS             complement(18179..18658)
                     /locus_tag="LAF92_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBI9792316.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            complement(18842..20260)
                     /gene="gltD"
                     /locus_tag="LAF92_003362"
     CDS             complement(18842..20260)
                     /gene="gltD"
                     /locus_tag="LAF92_003362"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBI9792317.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(20273..>20607)
                     /locus_tag="LAF92_003363"
     CDS             complement(20273..>20607)
                     /locus_tag="LAF92_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792318.1"
                     /translation="ILGKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDL
                     AIHEEHLRGLITEHVQHTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSA
                     AELRVQAQ"
BASE COUNT         4942 a         5187 c         5323 g         5155 t
ORIGIN      
        1 cataaatatt atcgtgctgg ataacccgat gactttccat ttcagtcagt ttctcgacag
       61 cgcattaggg caaatcgtcg gctgtgtgct cgcgttcacc gttattttgc tggtgcggga
      121 taaatcgcgc gacaggactg gacgtgtact gcttaatcag tttgtttctg ccgctgtttc
      181 cgcgatgact accaatgtgg cacgtcgtaa agagaaccac ctcccggcac tttatcagca
      241 gctgtttttg ctgatgaata agttcccagg ggatttgccg aaatttcgcc tggcgctgac
      301 gatgattatc gcccaccagc gcctgcgtga tgcgccgatc ccggttaacg aggatttatc
      361 ggcgtttcac cgacaaatgc gccgcacagc agaccatgtg atatctgccc gtagcgatga
      421 taaacgtcgt cggtactttg gtcagttgct ggaagaactg gaaatctacc aggaaaagct
      481 acgcatctgg caagcgccac cgcaggtgac ggaaccggtt catcggctgg cggggatgct
      541 ccataagtat caacatgcgt tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg
      601 ttttttcatg gctatactta gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc
      661 aacagatgaa tatttatacc tttgattttg atgagattga gagtcaggag gatttttatc
      721 gtgactttag ccaaaccttt ggtctggcga aagataaggt acgcgatctc gactcactat
      781 gggatgtgtt aatgaacgat gtcctgccgc taccacttga gattgaattt gttcatctgg
      841 gagagaaaac gcgtcgccgt tttggcgcgt taatattgct gtttgatgag gcagaggaag
      901 agctggaagg gcatttgcgt tttaatgttc gtcattagcg caaaaaaaag cccccgaacc
      961 gggggcaata tcgtcggaca agacgatgag ggtttatttg tacagttcag ccgtagcgtg
     1021 ccaggtgtca ccgctacgag cttcagtaat ctggtaggcc gttgcgcctt tctcttccgc
     1081 ttttttgttc agcatttcac gcatatccat tggcgaagac gccacaccac ttacggatac
     1141 ggtcccgatt gcttcacgat tttgtgcttg tgcagcatca atggagtcgg cagcgaatgc
     1201 accgaaagag agaacagaaa gtacgcttaa tgcagcaaca gtggttttga ttttcatgat
     1261 ttttacctcg acataatctt ttagctgggc ctttgtttcg tgacccatat cacaaaatca
     1321 agtatacact aattattgaa ctaattaata ccacgctaac tgtttcagtt gatactaagt
     1381 gttaaaaatg tgaattttaa taacaaaaaa gtattaaaaa cacggttatt tattataaat
     1441 accagttttt tcaatcagat aatgaatttt gcataatgtg ctttttttac cgtatgtatt
     1501 cattgtgtga atgatatgtc gcggtaaaac gcactattcg ttaaatgaat cgcaggggaa
     1561 agcaggggat tgagagggat aagcaacatt ttccccgccg tcagaaacga cggggcagag
     1621 attaaagctc ctggtcgaac agctctaaaa tcgcttcgta caggtctttg acggtgaaac
     1681 cgttagcagg ggtggtaaag atggtgtcat cgccagcgat ggtgcccaga ataccttctg
     1741 ctttgcccag tgagtccagc aggcgagcaa ttaactgcgc cgcgccaggg ctggtatgaa
     1801 tcacgacaac tgcatcgttg tagtcgatat ccagcaccag attcttcaat ggactggagg
     1861 tggttggtac acccagttca gctggcaggc agtaaaccat ttccattttg gcattgcgtg
     1921 tacgtacagc accaaacttg gtcaacatcc gcgagacttt agactgatta atattgtcaa
     1981 agccttgctc ctgcaacgcg gcgacgattt cgccctggga gctaaatttc tcttctttaa
     2041 gtaatgcttt aaatgcttta actagttctt cttgcttagc cgagcttcgc ataagtcacc
     2101 cgatatggtg gttgatacaa cattattgtg catacagatg aatttttatg caaacagtca
     2161 gccctgaaga aggctgaaat aatgttatga aagagcggga ttttatcaaa tttcgttatt
     2221 gagaaacatg cctgcgtcac ggcatgcaaa ttctgcttaa aagtaaatta attgttatca
     2281 aattgatgtt gttttggctg aacggtaggg tatattgtca ccacctgttg gaatgttgcg
     2341 ctaatgcata agcgactgtt aattacgtaa gttaggttcc tgattacggc aattaaatgc
     2401 ataaacgcta aacttgcgtg actacacatt cttgagatgt ggtcattgta aacggcaatt
     2461 ttgtggatta aggtcgcggc agcggagcaa catatcttag tttatcaata taataaggag
     2521 tttaggatga aagtcgcagt cctcggcgct gctggcggta ttggccaggc gcttgcacta
     2581 ctgttaaaaa cccaactgcc ttcaggttca gaactctctc tgtatgatat cgctccagtg
     2641 actcccggtg tggctgtcga tctgagccat atccctactg ctgtgaaaat caaaggtttt
     2701 tctggtgaag atgcgactcc ggcgctggaa ggcgcagatg tcgttcttat ctctgcaggt
     2761 gtagcgcgta aaccgggtat ggatcgttcc gacctgttta acgttaacgc cggcatcgtg
     2821 aaaaacctgg tacagcaagt ttcgaaaacc tgcccgaaag cgtgcattgg tattatcact
     2881 aacccggtta acaccacagt tgcgattgct gctgaagtgc tgaaaaaagc cggtgtttat
     2941 gacaaaaaca aactgttcgg cgttaccacg ctggatatca ttcgttccaa cacctttgtt
     3001 gcggaactga aaggcaaaca gccaggcgaa gttgaagtgc cggttattgg cggtcactct
     3061 ggtgttacca ttctgccgct gctgtcacag gttcctggcg ttagttttac cgagcaggaa
     3121 gtggctgatc tgaccaaacg tatccagaac gcaggtactg aagtggttga agcgaaagcc
     3181 ggtggcgggt ctgcaaccct gtctatgggc caggcagctg cacgttttgg tctgtctctg
     3241 gtacgcgcac tgcagggcga acaaggcgtt gtcgaatgtg cctatgttga aggcgacggt
     3301 cagtacgcac gtttcttctc tcaaccgctg ctgctgggta aaaacggcgt ggaagagcgt
     3361 aaatctatcg gtaccctgag cgcatttgaa cagagcgcac tggaaggtat gctggatacg
     3421 ctgaagaaag atatcgccct gggcgaagag ttcgttaata agtaattaat tagcgaataa
     3481 taaaaaaccg gagcacagac tccggttttt tgttttgagc actcgactta attggttgcc
     3541 ggatattcct gaatggtgac ctgcagcgtt aactgcttat catcacgcat cactacaacc
     3601 gggatcaccg aaccagggcg aatttctgcc acctgatcca tcgtctccag agcagagatg
     3661 gccggtttgt tatccaccga aataatcaga tcgttgacct gaatacccgc attcgccgcc
     3721 gggccgtcag gtgacacttc attaaccacg atcccttgca gttgatctat accaccgccc
     3781 tgcgcgtgca gtggtgcgat ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga
     3841 ccatcgcgga tcagcttatc cataattttg gttgctaact ggaaaggaat cgcaaagccg
     3901 ataccttccg gcgtttcgcc atcgttactc ttatcaaacg acagcgtgtt aatgcccatc
     3961 agttctccca gcgagttcac cagcgcgccg ccagagttac cgtggttaat ggaagcatcg
     4021 gtttgcagga agttttgccg cccggtcggg ttcagaccga ttcgacccgt agcactaata
     4081 atcccctggg taatggtctg cccgaggttg tacgggttac cgatcgccag taccacgtcg
     4141 ccaatgtgcg gtacgcgacg tgcattgatc ggaatggtag gtaaaccgcc agtggcatta
     4201 attttaagta ccgccagatc ggttagagag tcagatccca ccagcaatgc ttcaaataca
     4261 cgtccatcct gtaaggcgac gatgatctga tcggcgtcgt tgattacgtg tttattggtg
     4321 atgatataac cgcgttgatc catgattaca ccggatccca gggtgcggat ctcaagctgg
     4381 ttgtgagagt tggtgttcaa accacggttg taaacgttaa ccaccgctgg cgcggcgcgg
     4441 cgaaccgcca gattatagct ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa
     4501 agcgggttaa ggctgcgcag cgaaggcatg gcaaccagca gaatagcgcc gacaattaat
     4561 ccaatcgcaa cggaacgtaa gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga
     4621 acggcagcag cataccacga gttaaccgga catcacacgt aagcctgatg cccggtttac
     4681 gacattaacg catcagcaga tagatgcttt cattgccgcg tacaatttgc agggcgatga
     4741 tggccggttt tgccgccagc actttacgca tttcagcaat cgagttcacc cgatcgcggt
     4801 tgacgccaat gatcacatcg tctttttgca agccagcctg agcagctggg cttcctttga
     4861 caacttcatc gattttaata cctttgccgc catcttttag ctgaccatcg ctcaacgttg
     4921 caccttccag cgctggcgtg atcatttcag cgctggccga cgaagaggtg ctggtatcga
     4981 gcgtcacttc tacttccagt ggtttgccgt tacgcagcag gccaagcttc actttcgtgc
     5041 ccggctcggt ggtcgcgata cgagagcgca actcagcaaa gctattcagc ggtttgccgt
     5101 tgaggctggt aataatatcg cccgctttga cgcccgcttt tgccgagcca gaacctggca
     5161 acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt gaaggctttg gcgatatcgg
     5221 cactcatctc ggtgcctttg atgcctaaca aaccgcgttt gatttcacca aagtcgataa
     5281 gctgctgcgc cagtgttcgc gccatattac tggggatggc aaatccaatc ccgacgctcc
     5341 cgccgccagg cgcaaggatt gcagtgttga tgccaattaa ctcaccgtta aggtttaata
     5401 gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt ctggataaag ttttccagac
     5461 cttcaagatt caacccgctg cgtcctaatg cggaaacaat gccagaggtg gcggtttgcc
     5521 caaggccaaa tgggttaccg accgccacgg caaaatcacc gacgcgcaat ttatcggagt
     5581 cggcaatagc gatttgcgtt aatttgctcg ggttttgaat ttgtaacagg gcgatatcgc
     5641 tctggtcatc gctaccaatc agttttgcat caaactcgcg cccatcattg agctgaatac
     5701 tgattttctg tgcctgatta atcacatggt tgttggtcag cacatagcct ttactggcgt
     5761 tgatgatgac accggaacct aaaccttcga agggttgtgc aggttgatcc ggtaaatcat
     5821 caccaaaaaa ctttttgaat tcttccggga ttttctgtcc ctgactggcc gttccttcca
     5881 cccgtacgct caccactgcc ggaagcactt tttccagcat tggagccaga ctggggagag
     5941 gggcctgatc ggcaacctgg cctggaatcg acgcgacggc ctgaaatgac gccgagagag
     6001 ttaacccgac acttaacgct aatgcactca acagctgggt ttgttttttc attattcctg
     6061 ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt ttgatgttat tgaattttca
     6121 gacggctaac aatgagacac cagattaaat gatagtcggg gagggagaga agaggagggc
     6181 gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc gccagtacgc agcaggccgg
     6241 atgcgccttc agaatagtcg cgaggcatct gcaccggtgc ctgatcgttg ctggcttcag
     6301 actctgccag acgattacgg aacgggtttg cttcagcaga cagttccggc agcaggctgc
     6361 tggagctttt cgccatgtgc tgatacagct ggcgatagtc gtgcgccatg gtatccagta
     6421 attccgcgct gcgggcaaag tggctaacca gctcttcgcg atactcgtcc agttcagctt
     6481 tattcttttc cagttcgtac tgcaacgcct gttgctggcg tagtttacga ttaccaaaac
     6541 gcatggccac agcaccaata atgatgccga cgactaaccc aattagcgca tattcccagg
     6601 tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt tggcttcagg ctccatgcct
     6661 gcggctgatt atgccactat aaccgttaat tccacagaag tggaatcccg actgcatatc
     6721 gcgtagtgta gaacggcctt tttttcgtca accgtgaaca acggcgcacc gattattcaa
     6781 ggaataacaa taagatcatg caaagcgtta ccccaacatc gcaatacctg aaggcgctca
     6841 atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc cgtcagccgc ctggaaatta
     6901 tttatcaaga actcatcaat agcaggccac cagcccccag gacgagtggg ctaatggcgc
     6961 gggtcggtaa gctgtggggt aaacgcgaag acacaaagca tacgccagtg cgtggcttat
     7021 atatgtgggg cggtgtagga cgcgggaaaa cctggctgat ggaccttttc tatcaaagcc
     7081 tgccgggaga gcggaaacag cgcctgcact ttcaccgttt tatgctgcgg gtgcacgaag
     7141 agctaactgc cttacagggg cagaccgatc cgctggaaat tattgccgat cgctttaaag
     7201 ccgaaactga cgtgctctgt tttgacgaat tttttgtttc tgatattacc gacgccatgc
     7261 tacttggcgg tctgatgaaa gccctgttcg cccgcggcat taccctggta gcgacgtcaa
     7321 atattccgcc ggatgaactt tatcgaaatg gcctgcaacg tgcgcgtttt ctgcctgcaa
     7381 tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga cgctggtgtt gattatcgac
     7441 tgcgtacact cactcaggcg catctgtggc tttcgccact caacgatgaa acccgggcgc
     7501 agatggataa actatggttg gcgctggcgg gggcgaaacg agaaaattca ccgacgttag
     7561 aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa ccagacgctg gcggtctctt
     7621 ttactacgct gtgcgtcgac gcccgcagtc agcatgacta tattgcgctc tcacgtctct
     7681 ttcatacggt catgttgttt gatgtaccag ttatgacgcg gttgatggag agcgaagcgc
     7741 ggcgctttat tgcgctggtg gatgagtttt acgagcgcca tgtcaaatta gtggtgagtg
     7801 cagaagtgcc gctgtatgaa atttatcagg gcgatcggct gaagtttgag ttccagcgtt
     7861 gcctgtcacg tctgcaagag atgcaaagcg aagagtatct gaagcgcgag catttggcgg
     7921 gttaaaacct gtcacaaatc acaaaaaggg gtcgatcttt gaccccgact tctctataat
     7981 cctgcgaccc cacgttacaa gaaagttttt ttcccaaaac tttttgtgtg ctggcatagg
     8041 ctattcgaag gggtaggttt gccggacttt gtcgtgtgaa cctcaacaat tgaagacgtt
     8101 tgggtgttca ccaacgtgta actatttatt gggtaagctt ttaatgaaaa cttttacagc
     8161 taaaccagaa accgtaaaac gcgactggta tgttgttgac gcgaccggta aaactctggg
     8221 ccgtctggct actgaactgg ctcgtcgcct gcgcggtaag cacaaagcgg aatacactcc
     8281 gcacgtagat accggtgatt acatcatcgt tctgaacgct gacaaagttg ctgtaaccgg
     8341 caacaagcgt actgacaaag tgtactatca ccacaccggc cacatcggtg gtatcaaaca
     8401 agcgaccttt gaagagatga ttgctcgccg tcctgagcgt gtgattgaaa tcgcggttaa
     8461 aggcatgttg ccaaaaggcc cgctgggtcg tgctatgttc cgtaaactga aagtttacgc
     8521 gggtaacgag cacaaccacg cggcacagca accgcaagtt cttgacatct aatcgggatt
     8581 ataggcaatg gctgaaaatc aatactacgg cactggtcgc cgcaaaagtt ccgcagctcg
     8641 cgttttcatc aaaccgggca acggtaaaat cgtaatcaac caacgttctc tggaacagta
     8701 cttcggtcgt gaaactgctc gcatggtagt tcgtcagccg ctggaactgg tcgacatggt
     8761 tgagaaactg gacctgtaca tcaccgttaa aggtggtggt atctctggtc aggctggtgc
     8821 gatccgtcac ggtatcaccc gcgctctgat ggaatacgac gagtccctgc gttctgaact
     8881 gcgtaaagct ggcttcgtta ctcgtgacgc tcgtcaggtt gaacgtaaga aagtcggtct
     8941 gcgtaaagca cgtcgtcgtc cgcagttctc caaacgttaa ttggcttctg ctccggcaga
     9001 aaacaatttg cgaaaaaacc cgcttcggcg ggttttttta tggttaaatt ctgaatcagc
     9061 gtaaaaattg gaaagttgct ttttgctacc gtctgacaga caggcaaaac aaaaaccaca
     9121 tcgccaataa gggactaagt caactatttc agactaaagc gcatctcttt ttccccattt
     9181 ccggcatcga ctcaccacaa aggtcgcaaa atctggtaaa ctatcatcca attttctgcc
     9241 caaatgtcgg gtattgctca ttttttgttt gattttcgaa caaagagagt cgttccttac
     9301 tgggtaacac aacttctgac tggccacctg gtggctggta gcagtaaaaa ttctgactat
     9361 acctggaggt tttcatggct gtcgctgcca acaaacgttc ggtaatgacg ctgttttccg
     9421 gtcctactga catctatagc catcaggtcc gcattgtgct ggctgagaaa ggtgtaagtt
     9481 tcgagatcga acacgtggaa aaggacaatc cgcctcagga tctgattgac ctcaacccga
     9541 atcagagcgt tccgaccctg gtggatcgtg agctgaccct gtgggaatct cgcatcatta
     9601 tggaatatct ggatgagcgt ttcccgcatc cgccactgat gcctgtttac ccggtagctc
     9661 gcggtgaaag ccgtctgtac atgcatcgca tcgaaaaaga ctggtacacg ctgatgaaca
     9721 ccatcatcaa cggttcagct tctgaagcag atgccgcacg taagcaactg cgcgaagaac
     9781 tgctggcgat tgcgccggtc ttcggtcaga agccgtactt cctgagcgat gagttcagcc
     9841 tggtcgattg ctatcttgct ccgctgctgt ggcgtctgcc gcaactgggc atcgagttca
     9901 gcggcccggg tgcgaaagag ctgaaaggct atatgacccg cgtctttgag cgtgactctt
     9961 tccttgcttc tttaactgaa gcagaacgtg aaatgcgtct gggccggagt taatctgtat
    10021 ggatttgtca cagctaacac cacgtcgtcc ctatctgctg cgtgcattct atgagtggtt
    10081 gctggataac cagctcacgc cgcacttggt ggtggatgtg acgctccctg gcgtgcaggt
    10141 tcctatggaa tatgcgcgtg acgggcaaat cgtactcaac attgcgccgc gtgctgtcgg
    10201 caatctggaa ctggcgaatg atgaggtacg ctttaacgcg cgctttggcg gcattccgcg
    10261 tcaggtttct gtgccgctgg ctgccgtgct ggctatctac gcccgtgaaa atggcgcagg
    10321 cacgatgttt gagcctgaag ctgcctacga tgaagatacc agcatcatga atgatgaaga
    10381 ggcatcggca gacaacgaaa ccgttatgtc ggttattgat ggcgacaagc cagatcacga
    10441 tgatgacact catcctgacg atgaacctcc gcagccacca cgcggtggtc gaccggcatt
    10501 acgcgttgtg aagtaataca aaaacaggcc ctggtggcct gttttgtctt ttaatggctt
    10561 aataaaatag cgtgattgtg gcaatcatat tcaccacgaa accgactgcc atggggatcg
    10621 ctgttcgctt caccaccgca aaaggcgaaa tgcctgcaat tcctgacgta acgaccacca
    10681 ccgcagtaat cggcgaaacc gcgcgcgcta gcgtcgtggc aaaatgcatc ggcataatca
    10741 ttacaaccgc tggtacatgt agtccggctg cgatattcgg aataagactg gcaaatgaca
    10801 taaacggcgc attgccagag cccatcacaa tggcacaaat ggcaatgact agcgccataa
    10861 taatcatcac gccaataccg cccagaccag aatgctccgc acccctgata accgcatcaa
    10921 cagtgccaat cgtcgttaag cctttcgcaa atatttcacc cgcgaccacc agcgttacca
    10981 cgttggcaaa ctgcgtaccc atgccgtcaa aaaacgcctg cacatcgtcc attgtttcgc
    11041 gcaagttatg cttgcggaag aactcaacaa acatcgtcac cgtcaaactc agtaacatca
    11101 ccaccaccag atgcagttct gactgcatca gcccgatgtg ggcgaggaac agcgagccga
    11161 gcatcaagat taacggcatc acaggtaaaa tggcgtaata gagcggcggg acattatcga
    11221 gagcttttag ctctgcctgt tcgtgattga tatttttatc ttttttgtca aaagcgcgtt
    11281 gcacgaaaaa gtgggagatc gccaccgaga taatgacgca agaggcgacc ggaagctggt
    11341 agtggaagaa gtaagtggca attttcattc ccgcgacctg ggctgcaaaa atggagttcg
    11401 tttccagaat cccccactca atggacatcg tggttgcgat aaccgccacc gcagagaggc
    11461 gacttactcc cagactcacc agcgtcggaa ataaggtgac catcagcaac atacccaggc
    11521 cggaggcgct ggtaataaac tgcgccatga tttggccgat gacgtaagtt gccgacagaa
    11581 taatatacgg cgagcgaatg agttttaacg ggcggcttag caagctcacc atcgcgcgac
    11641 tggctcctgt gcgctccatg tagcgggcat aaccgcccac cgccataatc gacagcccca
    11701 atccggcaat gcggttggac agcatgcgca ggatttcatt ataaatatcg acgatcaagt
    11761 agccgctgct tttggcggga tcgagtacac caccgaaccc taaccagaca ccgcacatca
    11821 tcaggaagat acctgcggca gccagcacca cctgaggttt gtagtttttc aggatcaaat
    11881 agcccatcgt aattaatacg atgacagata taattatgcc gaacattgtt gaactccgtg
    11941 tcaaaagaaa acggtcaatc ccataaacgg cagattgaaa acaatgatgt tatatttttt
    12001 gcaagtctat ttatggtgcg gatgtcgtgt ttttaattgt aggtgaggtg gtttttcatt
    12061 aaaaaatatg cgcttatgat tattttgtaa gaacacattc ataatattca taatgctcgt
    12121 gaatagtctt atgaataatt caaacgggat gtttttatct gcgttacatt aatttttcgc
    12181 aatagttaat tattccgtta attatggtaa tgatgaggca caaagagaaa aacctgccat
    12241 tttcccctac tttcaatcct gtgataggat gtcactgatg atgttaatca cactgacctt
    12301 acagaatggg ccttatgaac gcatttgatt cgcaaaccga agattcttca cctgcaattg
    12361 gtcgcaactt gcgtagccgc ccgctggcgc gtaaaaaact ctccgaaatg gtggaagaag
    12421 agctggaaca gatgatccgc cgtcgtgaat ttggcgaagg tgaacaatta ccgtctgaac
    12481 gcgaactgat ggcgttcttt aacgtcgggc gtccttctgt gcgcgaagcg ctggcagcgt
    12541 taaaacgcaa aggtctggtg caaataaaca acggcgaacg cgctcgcgtc tcgcgtcctt
    12601 ctgcggacac tatcatcggt gagctttccg gcatggcgaa agatttcctt tctcatcctg
    12661 gtgggattgc ccatttcgaa caattacgtc tgttctttga atccagcctg gtgcgctatg
    12721 cggctgaaca cgccaccgat gagcaaatcg atttgctggc aaaagcactg gaaatcaaca
    12781 gtcagtcgct ggataacaac gcggcattca ttcgttcaga cgttgatttc caccgcgtgc
    12841 tggcggagat cccaggtaac ccaatcttca tggcgatcca cgttgccctg ctcgactggc
    12901 taatcgccgc acgtccaacc gtggctgatc aggctctgca cgagcataac aacgttagct
    12961 atcaacagca tattgcgatc gttgacgcga tccgccgcca cgatcctgac gaagccgatc
    13021 gtgcgttgca atcgcatctc aacagcgtct ctgctacctg gcacgctttc ggtcagacca
    13081 ccaacaaaaa gaaataatgc cactttagtg aagcagatcg cattataagc tttctgtatg
    13141 gggtgttgct taattgatct ggtataacag gtataaaggt atatcgttta tcagacaagc
    13201 atcacttcag aggtatttat ggcaacgaat ttacgtggcg taatggctgc actcctgact
    13261 ccttttgacc aacaacaagc actggataaa gcgagtctgc gtcgcctggt tcagttcaat
    13321 attcagcagg gcatcgacgg tttatacgtg ggtggttcga ccggcgaggc ctttgtacaa
    13381 agcctttccg agcgtgaaca ggtactggaa atcgtcgccg aagaggcgaa aggtaagatt
    13441 aaactcatcg cccacgtcgg ttgcgtcagt accgccgaaa gccaacaact tgcggcatcg
    13501 gctaaacgtt atggcttcga tgccgtctcc gccgtcacgc cgttctacta tcctttcagc
    13561 tttgaagaac actgcgatca ctatcgggca attattgatt cggcggatgg tttgccgatg
    13621 gtggtgtaca acattccagc cctgagtggg gtaaaactga ccctggatca gatcaacaca
    13681 cttgttacgt tgcctggcgt aggtgcgctg aaacagacct ctggcgatct ctatcagatg
    13741 gagcagatcc gtcgtgaaca tcctgatctg gtgctctata acggttacga cgaaatcttc
    13801 gcctctggtc ttctggcggg cgctgatggt ggtatcggta gtacctacaa catcatgggc
    13861 tggcgttatc aggggattgt gaaggcgctg aaagaaggcg atatccagac cgcgcagaag
    13921 ctgcaaaccg aatgtaataa agtcattgat ttactgatca aaacgggcgt attccgcggc
    13981 ctgaaaactg tcctgcatta tatggatgtc gtttctgtgc cgctgtgccg caaaccgttt
    14041 ggtccggtag atgaaaaata tctgccagaa ctgaaggcgc tggcccagca gttgatgcaa
    14101 gagcgcgggt gagttgtttc ccctcgctcg cccctaccgg gtgaggggaa ataaacgcat
    14161 ctgtacccta caattttcat accaaagcgt gtgggcatcg cccaccgcgg gagactcaca
    14221 atgagtacta caacccagaa tatcccgtgg tatcgccatc tcaatcgtgc acaatggcgg
    14281 gcattttccg ctgcctggtt gggatatctg cttgacggtt ttgatttcgt tttaatcgcc
    14341 ctggtactca ccgaagtaca gggtgaattc gggctgacga cggtgcaggc ggcaagtctg
    14401 atctccgcag cctttatctc tcgctggttc ggcggcctga tgctcggcgc tatgggtgac
    14461 cgctacgggc gtcgtctggc aatggtcacc agcatcgttc tcttctcggc cgggacgctg
    14521 gcctgcggct ttgcgccagg ctacatcacc atgtttatcg ctcgtctggt catcggcatg
    14581 gggatggcgg gtgaatacgg ttccagcgcc acctatgtca ttgaaagctg gccaaaacat
    14641 ctgcgtaaca aagccagtgg ttttttgatt tcaggcttct ctgtgggggc cgtcgttgcc
    14701 gctcaggtct atagcctggt ggttccggtc tggggttggc gtgcgctgtt ctttatcggc
    14761 attttgccaa tcatctttgc tctctggctg cgtaaaaaca tcccggaagc ggaagactgg
    14821 aaagagaaac acgcaggtaa agcaccagta cgcacaatgg tggatattct ctaccgtggt
    14881 gaacatcgca ttgccaatat cgtaatgaca ctggcggcgg ctactgcgct gtggttctgc
    14941 ttcgccggta acctgcaaaa tgctgcaatc gtcgctgttc tcgggctgtt atgcgccgca
    15001 atctttatca gctttatggt gcaaagtacg ggtaaacgct ggccaacggg cgtaatgctg
    15061 atggtggtcg tgttgtttgc tttcctctac tcatggccga ttcaggcgct gctgccaacg
    15121 tatctgaaaa ccgatctggc ttataacccg catactgtcg ccaatgtgct gttctttagt
    15181 ggctttggcg cggccgtggg atgctgcgta ggtggcttcc tcggtgactg gctgggaacc
    15241 cgcaaagcgt acgtttgtag cctgctggcc tcgcagctgc tgattattcc ggtatttgcg
    15301 attggcggcg caaacgtctg ggtgctcggt ctgttactgt tcttccagca aatgcttgga
    15361 caagggatcg ccgggatctt accgaaactg attggcggtt atttcgatac cgaccagcgt
    15421 gcagcgggcc tgggctttac ctacaacgtt ggcgcattgg gcggcgcact ggccccaatc
    15481 atcggcgcgt tgatcgctca acgtctggat ctgggtactg cgctggcatc gctctcgttc
    15541 agtctgacgt tcgtggtgat cctgctgatt ggtctggata tgccttcccg cgttcagcgt
    15601 tggctgcgcc cggaagcatt gcgtactcat gacgctatcg acggcaaacc atttagcggt
    15661 gccgtgccat ttggcagcgc caaaaacgat ttagtcaaaa ccaaaagtta atcctgttgc
    15721 ccggtctatg taccgggcct ttcgctaagg gaagatgtat gtcgttactt gcacaactgg
    15781 atcaaaaaat cgctgctaac ggtggcctga ttgtctcctg ccagccggtt ccggacagtc
    15841 cgctcgataa acccgaaatc gtcgccgcca tggcattagc ggcagagcag gcgggcgcgg
    15901 ttgccattcg cattgaaggt gtggcaaatc tgcaagccac gcgtgcggtg gtgagcgtgc
    15961 caattatcgg gattgtgaaa cgcgatctgg aggattctcc ggtacgcatc acggcctata
    16021 ttgaagatgt cgatgcgctg gcgcaggcgg gcgcggacat tatcgccatt gacggcaccg
    16081 accgcccgcg tccggtgcct gttgaaacgc tgctggcacg tattcaccat cacggtttac
    16141 tggcgatgac cgactgctca acgccggaag acggcctggc atgccaaaag ctgggagccg
    16201 aaattattgg cactacgctt tctggctata ccacgcctga aacgccagaa gagccggatc
    16261 tggcgctggt gaaaacgttg agcgacgccg gatgtcgggt gattgccgaa gggcgttaca
    16321 acacgcctgc tcaggcggca gatgcgatgc gccacggcgc gtgggcggtg acggtcggtt
    16381 ctgccattac gcgtcttgaa cacatttgtc agtggtacaa cacagcgatg aaaaaggcgg
    16441 tgctatgacc acgctggcga ttgatatcgg cggtactaaa cttgccgccg cgctgattgg
    16501 cgctgacggg cagatccgcg atcgtcgtga acttcctacg ccagccagcc agacaccaga
    16561 agccttgcgt gatgccttat ccgcattagt ctctccgttg caagctcatg cgcagcgggt
    16621 tgccatcgct tcaaccggaa ttattcgcga cggcagtttg ctggcgctga atccgcataa
    16681 tctcggtgga ttgctacact ttccgttagt caaaacgctg gaacaactta ccaatttgcc
    16741 gaccattgcc attaacgacg cgcaggctgc agcatgggcg gagtatcagg cgctggaagg
    16801 cgatataacc gatatggtct ttatcaccgt ttccactggc gtcggcggcg gtgtagtgag
    16861 cggcggcaaa ctgcttaccg gccctggcgg tctggcgggg cacatcgggc atacgcttgc
    16921 cgatccacac ggcccagtct gcggctgtgg acgcacaggt tgcgtggaag caattgcttc
    16981 tggtcgcggc attgcggcgg cagcgcaggg cgagctggct ggcgcggatg ctagaactat
    17041 tttcacacgc gccgggcagg gcgatgagca ggcgcagcaa ctgattcatc tctccgcacg
    17101 tacgcttgca aggctgatcg ctgatattaa agccacaact gattgccagt gcgtggtggt
    17161 tggtggcagc gttggtctgg cagaagggta tctggcgttg gtggaaatgt atctggcgca
    17221 ggagccagcg gcatttcatg ttgatttact ggcggcgcac taccgccatg atgcaggttt
    17281 acttggggct gcgctgttgg cccagggaga aaaattatga tgatgggtga agtacagtca
    17341 ttaccgtctg ctgggttaca tcctgcgtta caggacgcgt taacgctggc cttagctgcc
    17401 agaccgcaag aaaaagcgcc gggtcgttac gaattacagg gcgacaatat ttttatgaat
    17461 gtcatgacgt ttaacactca atcgcccgtc gagaaaaaag cggaattgca cgagcaatac
    17521 attgatatcc agctgttatt aaacggtgag gaacggattc tgtttggcat ggcaggcact
    17581 gcgcgccaat gtgaagagtt ccaccatgaa gatgattacc agctttgtag cgccattgaa
    17641 aacgagcaaa ccatcatcct gaaaccggga atgttcgccg tgtttatgcc aggagagccg
    17701 cataagccgg gatgcgttgt tggcgagccg ggtgagatta aaaaggtggt ggtgaaggtt
    17761 aaggctgatt taatggctta aatttattag atcaggtgtg acctgaccca tctttggcac
    17821 agagccaaac ctgaactgac ggaccgctct gtgccattac ctgacgttgt attcttttat
    17881 gttaacaccc ttgatactgt atatacctgt ccgcttaatt cgcttgccct ttttattcgt
    17941 aatagttgct gtaagtgtac tgcagcgaat ggaatgacgt tcatcaatca ccagccgaat
    18001 tgcttctgaa gcatcttcca cctctagaag gtcgtgagtc ttatttaagc cgaatggaac
    18061 aaggtggaag gcagtttttc ctgatgattt tctggttatt tttactggat acaacacatc
    18121 accactttta tggattaatt caagctgttc aatgtaaatc atggcaactt tccttcgttt
    18181 atttgcatga atatacaaac cgaaaatagg agatgcaata caaaagctgt ggaaatatca
    18241 aacagcctct caatttggca gacagcgtct gccaaatctt tgccgatggg aattctgcag
    18301 atggtgtctg cggaattccc aatggtgtta attcgccaga cactgtctgg cgaatttcaa
    18361 catgttgaaa aaagaggtaa aaaagcctca tttggcgtaa gttttttgca gagaacccac
    18421 gcttatacct tagacttaaa tcctttgata gtcgcttgat tagctgcgca ccatacgcag
    18481 ccctggcctc gccaccttgt tcaaattcga caattcgtcg gccaatttcc cagtatgtcg
    18541 cggtcatcag cgcgttaacg ctacgtaccg tttccgtccg ggcgctatcg accagatgaa
    18601 taataccgtc gtggatttgc tggtagcctg ttgaggttct ctctgagagc gattccatta
    18661 tctgtccctt tatatggcgc tgcgttgaat aatggaatca gctatacgcc gggcatctac
    18721 cggtaacaat ccagatgagc gtattacatt ggttttcttc agattatgcg aggcgctttc
    18781 cagaaagaaa ggagaatttc agcgccgact cttttctgag gaggcggcgc tgggacagag
    18841 cttaaacttc cagccagttc ataataccgt ctgccgcctt acgaccttcg gcaatagcgg
    18901 ttaccaccag atcggaacca cggacgatat cgccgccagc aaagattttc gggttgctgg
    18961 tctggaaggc gttgtcgctg ccttccgggg cgatgatgcg gccttgtgaa tccagctcga
    19021 cgctgtgttt tgccagccat tccatgttgt gtggacggaa accaaacgcc atgatcactg
    19081 catctgccgg aacgatatgt tcggaacctg caacgatctc cgcgcggcga cggcctttgg
    19141 catccggttc gcccatttcg gtacgtacca tttttacgcc gctgacttta ccgttaccgt
    19201 tcacttcaat ccccagtggc tggacgttga atttgaactc tacgccttct tcccgcgcgt
    19261 ttttcacttc gcggcgggaa cccggcatgt tctcttcatc acgacgatag gcacaggtaa
    19321 cgtgcttcgc tccctggcgc acggacgtac gcacgcagtc catcgcagtg tcgccaccgc
    19381 caaggaccac cacgcgtttg ccttccatgc tgacgaacgg ttcgtcgcgg gtttcaccaa
    19441 agcccattaa ctgtttggtg ttggcgataa ggaacggcag cgctgcgtac acgccatcgg
    19501 cgtcttcgtt ttccagcccg ccgcgcattg actgataagt cccgacgcca aggaagacgg
    19561 catcgtaatc actcagcaga tcgtccagct gcacgtcgcg gcccacttcg gtattgagtt
    19621 tgaattcaat acccatgccg gtgaagattt cacggcgacg cgtcattacc tctttttcca
    19681 gcttgaaggc cggaataccg aaggtcagca gcccgccaat ttctggatga cggtcgaaga
    19741 caacggcttt tacgccgtta cgcgtcagga catccgcaca cgccagacct gccgggcctg
    19801 cgccgataat cgccactttt ttaccggttt gcttcacgcc ggacatatcc ggacgccagc
    19861 ccatttcgaa cgctttatca ttgatataac gctcaatgtt gccgatggtc accgcgccaa
    19921 actcatcgtt cagagtgcag gaaccttcgc acagacggtc ttgcgggcag actcgtccgc
    19981 aaacttccgg cagggtgttg gtctggtgcg acagttccgc cgcttcaaaa atacgcccct
    20041 cgttggcgag cttcagccag ttcgggatgt agttgtgtac cggacatttc cactcgcagt
    20101 atgggttgcc gcacgacagg cagcggtcag cctgcgcttt ggcctggcct tcggaaaacg
    20161 gctcgtaaat ttcaacaaac tcaattttgc ggatcttcag cggtttcttt ggcggatcaa
    20221 cgcgctgcag gtcgataaat tgataaacat tctgactcat tgttgctacc ccttactgcg
    20281 cctgcacgcg caactctgct gcgctacgac tacgatgacc cagcagtgct tttacatcac
    20341 tggacttcgg tttaaccagc gcaaatttag tggcgaaggt tgaccagttc gccagaatct
    20401 cttcaccgcg ctgagagcca gtatgctgaa catgctcggt gataagaccg cgcagatgct
    20461 cttcatggat cgccagatcg tcaacgctta agacctcgac cagttccggg ttaacgcgtt
    20521 tgcggaaatc accgctttca tcgagaacgt aagcgaagcc gccggtcatg cccgcaccga
    20581 agttaacgcc ggttttaccc agaatac
//
LOCUS       DAEVZA010000092        20016 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEVZA010000092 DAEVZA010000000
VERSION     DAEVZA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.092"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..616
                     /locus_tag="LAF92_003364"
     CDS             <1..616
                     /locus_tag="LAF92_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9792319.1"
                     /translation="PMDIHSYHCEGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYN
                     QELTEVAELITSPEAIKNNLAVAFLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHA
                     SLQQRWSVADMAATIPCSEAWLRRLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVG
                     EVADTLNFFDSFHFSKAFKHKFGYAPSAVLKNTDQHPTDASPHN"
     gene            complement(613..1427)
                     /locus_tag="LAF92_003365"
                     /pseudo
     CDS             complement(613..1427)
                     /locus_tag="LAF92_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1427..3043)
                     /locus_tag="LAF92_003366"
     CDS             complement(1427..3043)
                     /locus_tag="LAF92_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBI9792320.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3339..4055
                     /locus_tag="LAF92_003367"
     CDS             3339..4055
                     /locus_tag="LAF92_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI9792321.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4052..5713)
                     /locus_tag="LAF92_003368"
     CDS             complement(4052..5713)
                     /locus_tag="LAF92_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBI9792322.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(5910..6338)
                     /gene="ibpB"
                     /locus_tag="LAF92_003369"
     CDS             complement(5910..6338)
                     /gene="ibpB"
                     /locus_tag="LAF92_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBI9792323.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6450..6863)
                     /gene="ibpA"
                     /locus_tag="LAF92_003370"
     CDS             complement(6450..6863)
                     /gene="ibpA"
                     /locus_tag="LAF92_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBI9792324.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(6857..6929)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7169..7501
                     /locus_tag="LAF92_003371"
     CDS             7169..7501
                     /locus_tag="LAF92_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBI9792325.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7503..8717)
                     /locus_tag="LAF92_003372"
     CDS             complement(7503..8717)
                     /locus_tag="LAF92_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBI9792326.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPFNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            8818..9882
                     /gene="cbrA"
                     /locus_tag="LAF92_003373"
     CDS             8818..9882
                     /gene="cbrA"
                     /locus_tag="LAF92_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBI9792327.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRNIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(9879..11216)
                     /locus_tag="LAF92_003374"
     CDS             complement(9879..11216)
                     /locus_tag="LAF92_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9792328.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11291..12439)
                     /gene="dgoD"
                     /locus_tag="LAF92_003375"
     CDS             complement(11291..12439)
                     /gene="dgoD"
                     /locus_tag="LAF92_003375"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBI9792329.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(12436..13053)
                     /gene="dgoA"
                     /locus_tag="LAF92_003376"
     CDS             complement(12436..13053)
                     /gene="dgoA"
                     /locus_tag="LAF92_003376"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBI9792330.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13037..13915)
                     /gene="dgoK"
                     /locus_tag="LAF92_003377"
     CDS             complement(13037..13915)
                     /gene="dgoK"
                     /locus_tag="LAF92_003377"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBI9792331.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(13912..14601)
                     /gene="dgoR"
                     /locus_tag="LAF92_003378"
     CDS             complement(13912..14601)
                     /gene="dgoR"
                     /locus_tag="LAF92_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBI9792332.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14879..15535
                     /locus_tag="LAF92_003379"
     CDS             14879..15535
                     /locus_tag="LAF92_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792333.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITAGLVFNTASPPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGGEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYHWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKASDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(15579..16391)
                     /gene="yidA"
                     /locus_tag="LAF92_003380"
     CDS             complement(15579..16391)
                     /gene="yidA"
                     /locus_tag="LAF92_003380"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBI9792334.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAISRIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16506..16904)
                     /locus_tag="LAF92_003381"
     CDS             complement(16506..16904)
                     /locus_tag="LAF92_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBI9792335.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17144..19558)
                     /gene="gyrB"
                     /locus_tag="LAF92_003382"
     CDS             complement(17144..19558)
                     /gene="gyrB"
                     /locus_tag="LAF92_003382"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBI9792336.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19587..>20016)
                     /gene="recF"
                     /locus_tag="LAF92_003383"
     CDS             complement(19587..>20016)
                     /gene="recF"
                     /locus_tag="LAF92_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HBI9792337.1"
                     /translation="LTFSFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIR
                     ADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLA
                     SRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD"
BASE COUNT         4916 a         5352 c         5045 g         4703 t
ORIGIN      
        1 gccaatggat attcactctt atcactgtga aggtttagtc tgggaacagt actggatgga
       61 atttaccccc accagtatga tggatattcc cgttggtcag caaagcgtta tttataatgg
      121 cgaaatttat aatcaggaac tcaccgaagt tgctgagtta ataacttcac cagaagcaat
      181 aaaaaataat ctggcagtcg cttttctgac gaaaattatt tatcagtgga tttgtcttat
      241 gtacgcagat ggtaaaaaag atccacaacg gcggcaaatt gaaaaattaa ttgccacttt
      301 acatgccagt ctgcaacaac gctggagcgt agctgatatg gctgccacga tcccctgtag
      361 cgaagcctgg ttgcgtcgtc tgtttttacg ctataccggc aagacgccga aagaatatta
      421 cctcgatgcg cgtctggatc tggcgctatc gctattaaag caacaaggaa actcggttgg
      481 cgaagtcgct gatacgctca acttcttcga ctcctttcat ttcagcaaag cctttaaaca
      541 taaatttggt tatgcgccgt cagccgtgct aaagaatacg gaccagcacc caacggatgc
      601 cagtccacac aattaacgta actctggcca gtacgctttg ttggcctcca gcaactcatc
      661 cagaatagct ttggcgaccg aggcgctcgg taccgttttc gacaacgtaa tcgcctgcca
      721 caggtgctga tatgaacgct gttcccaggc gtccacgacc agtttttcca ccgccacttg
      781 ctgactcatt aaccctttct gaaactgcgg gatatcgccg accaccagtg gttcaggccg
      841 tagtagcgca cgcgcatctg tttgcgatct tgcagcccaa caatttgcgc catccggctt
      901 tcaataccga ttggcatgtc acagatgttg aggattttcg cattcgggcg cagacgacgc
      961 gtggcttctg cgacaatggc tgccgggttg gagtagttga gcatccaggc atttggtgaa
     1021 tatttttcca tataatccac cagttccagg acgccgccaa tggaacgcat gccgtacgct
     1081 attccgccgg gtccgcaagt ttcctgacca acaacaccgt ggcgcagtgg gattttttca
     1141 tccagttcgc gcatcgggta cttgccgacg cggatgtgcg ccatgacaaa atcaacgtcg
     1201 ctgaatgcca cttcaggatc ggtggtgtaa ctaaacgcaa tgtccggcgc tttctcttta
     1261 aggatgactt tacaggcttc ggcaatcact tcctgccgcg caccatcgtt atcataaaat
     1321 ttcagtgcac gaagcgggaa acggtcctga ttcaccagga gcatcaacac gatgcctggc
     1381 gtaaaggtgc ttccaccgcc tgcgacaacc actgagaatt tggtcattat actgcctccg
     1441 taatggcaac attttctgct gattgatgag aattaattaa gctatcgagc tgttcacgca
     1501 gctgggatac atgcagacca atgatcacct gaatggcatc gccactacgg aagacgccgt
     1561 gcgctcccag ctttttaaag acttcgtcat ccagcgtttg tgacatgtca tgcagtgcaa
     1621 tgcgtagacg cgtcgcacaa ttgttaatgc tggagatatt gccgaccccg cccagggctt
     1681 gcaggatacc ggcagcctga tccagctctt tttttggctc tgccgcggtg gtttggcctc
     1741 gcgaggcttt gtattcggct tttgagtaga gtttcacttc cgcatcttca cgtcccggcg
     1801 tgcacatgtt gaactgcaga atcagggtgc ggaaaaccac gaagtacagc agggtaaagc
     1861 acaacccaat ggcgatttgg gtcagcatca tatccgcgtg gttgctgaac atcgggatcc
     1921 agttttgcgg taaaacctgg tcaatcagac ctccgcccat gttgcccacc acaccaaaga
     1981 gatacatcac ggtcgacatt gaggccgcaa gcacagcgtg taccgcaaac agcaacggtg
     2041 aaatgaacag gaaggtaaat tccagcggtt cggtaattcc caccagcatg gcggttaagg
     2101 ttgcgggaat cagcaagccc gccactttta cccgattttc cggtgcggca gtgaagtaca
     2161 tcgctaaaga aatgcccacg gcaccaaaga tttttgagtt accgtgcagg gcaaaaccgc
     2221 cttccgggaa caacgatttc agcggctcgg cgctcagact gaactcttgc agatgctgcg
     2281 cccagtacat ctgaatgccg ccttcaacag ctgccggacc aaagataaac tgtccgtaga
     2341 tgaagtggtg taaaccggtt gggatcagaa tacgttcgag gaaggtgtaa acccacaccc
     2401 caagcgcacc cgccgaacgc aggaacgctt gcagagattc aatccccatt tgtacttttg
     2461 gccagccgag caatgtcagc caggcacagg ggatcatcac caggaaggcg ataatcacca
     2521 cataagacgt cccctggaaa atgccgagaa aaaccggcag ttttttatcg aacagacggt
     2581 tatgcagcgc cgtcacaatg ccggaaatga taattgcgcc gataatgctg gtatcgaggg
     2641 ttttaatccc ggccatcatt gtcagaccgc tacctgccac cgcgtcctga gtgaaatcga
     2701 cgccgaagta gcttccccag gtcattccca tcgcattgat gaaatagttc caggtcagga
     2761 aactcaccat caccgccaga caagcacgcc cctgcgcttg cttagcaagg ccaatgggta
     2821 aaccgacagc aaaaatcagc ggcatattac ggaataccgt ccaaccgccc tcttcaataa
     2881 tgtgtacgat ttgcgcgaat aaactgttcg gatcggtcag tgattccccg acaaacatcg
     2941 ggttttgcag caagatggca agacccacca caatcccggc gaagggaaac agcagcactg
     3001 gcgtgaacat cgcgccgcca aagcgttgaa tttgactgag catttccaca tcctcttttc
     3061 tcaattctgt aagtaggaag cctgattggt tatgcggcag gcctggagcg aggataggaa
     3121 gatgcgatcg gcagaacatg acgacccaat gggattcgtg atcgctttca tgcttttcgt
     3181 tgggcggtag agatctacgt tttcgcgaaa aagtagacat ctgtgagcac tggtacacag
     3241 catcgaacac atctttaaaa aaaagatgtt ttttcaatcg attaagcaga acttgtgggc
     3301 gcattacccg tgcttgcagg caaaaaagag atctagagat gatctacaaa agcattgcgg
     3361 agcggttaag aattcgactt aattccgcag atttcacgct aaatagcctt cttcccggtg
     3421 aaaaaaagtt ggcggaagaa tttgcggtat cgcggatgac catccgtaaa gccattgacc
     3481 tgctggtagc gtggggattg gtggtccgcc gccacggcag cggcacttac ctggtgcgca
     3541 aagatgtact gcatcaaacc gccagcctga ccgggctggt ggaggtgtta aaacggcagg
     3601 gaaaaacggt caccagccag gtgctgattt ttgaaatcat gcctgcgcct ccggccattg
     3661 ccagccagtt acggattcaa atcaacgagc agatctactt ctcccgtcgc gttcgttttg
     3721 tggaagggaa accgctgatg ctggaagaca gctatatgcc ggtaaaactg ttccgtaatc
     3781 tttcgctgca acatctggaa gggtcgaagt ttgaatatat tgaacaagag tgcgggattt
     3841 tgattggcgg taattatgaa agcctgacgc cagtgctcgc cgatagactg ctggcgcggc
     3901 aaatgaaggt agcggaacac acgccactgc tgcggatcac ctcgttgtca tatagcgaga
     3961 gcggggagtt tttgaattat tcagtgatgt tcagaaatgc cagcgaatac caggtggagt
     4021 accatttacg gcgactccac ccggaaaaga gttaaccgat actccagaag agcaccgcca
     4081 gtaattgggg ggtgataatg cgcaggaaca tcactaacgg atagacagtg gcgtaagaga
     4141 gcgccgccgc accgctggtt ggatgaagat tattagcaaa cgccagcgcc ggaggatcgg
     4201 tcatggaacc tgccagcatc ccgcacatgg tcaggtaatt cattttggct aacatccgcg
     4261 ccagaatgcc aacagtaatc agcggaacgg cggtgatcag ggcaccataa ccaatccagc
     4321 ttagcccttc gccattgacc agggtattta caaaatcccc accagatttc agaccaacga
     4381 ccgagaggaa cagcacgatc cccagctccc gcagcgcgag gttggcactt ggcggcataa
     4441 accagtacag cttgccgata ctgccgatac gcccgaggat caacgccata atcagcggac
     4501 cgcccgccag ccccagtttc aacgcggccg ggaatcctgg cacaaagacg ggaatagaac
     4561 ctaacaatac gccaagcccg atgccaataa acactggcag catctgaacc tgttgcagtt
     4621 tttgctgcgc attccccagc acattggcaa cggcatcaat tgcggacgga cgccccacca
     4681 ggttgaggat atcgccgaac tgcaggctga tatcgccact ggcgaccagt tcgaccccgg
     4741 cacggttcag gcgcgagata acaacgtcat agcgttcttt aaagtgcagg tcgcgaatac
     4801 gttttccgag cacgttttca ttggtgacca ccacacgctc gacgcgcaaa tcagtgcctt
     4861 tcgtggacag cgaagtatcg acctcctgac caatcaccag ttgcgcatta tgtaaatccg
     4921 ctggctgacc caccagatgc agcaaatcgc ccagttgaat aatggtatct ggcgaaggaa
     4981 cttttagggt ttcttcgcgt ttcagacgcg agcagataat tttgtcgccg ttgagaatcg
     5041 gtacatcttt aatggctaaa tcatgcaggt tagggttctc aacgcgaata ttgatagtct
     5101 tgatcagcgc gccgccattg gtgcgtgaag actcgtgctg ctgagcttct gtctcgacat
     5161 tgacgcggaa aataacccgc aacatccaca tggtgaacaa aatcccgcaa atgccgaatg
     5221 gatacgccat cgcgtaactc atccccatct gatcgaccat ttccattggt gtacccaggt
     5281 cgcgcaaaat ctgctgccct gcccccagcg ctggcgtatt ggtaaccgca ccggagaaaa
     5341 tccccagcac taccggcagt ggaatatcaa acagtttatg caggatggcg gtaaccagac
     5401 caccgatgat gacgatcaga acagcaaaca ggttgaggcg taatccggag acgcgcaatg
     5461 aggcaaagaa gcccggccct acctgaatcc cgatagtata aacgaacagg atcaggccaa
     5521 attcctgaat aacatgcagc atatcgctac ttaatgtcat ccccgcctga gaaacaaaat
     5581 ggccgacgat gatcccacca aacagcacgc cgccaatacc taatcctatg ccgcgaaatt
     5641 tgacattgcc gataaacaaa ccgacgactg ccaccaaagc cagaatactg accgttaatg
     5701 ctatatcact catcgtccat ttctcttgag ataacatttt taattgctac ggattatggc
     5761 tgaatcggtg aagcagattt gccttatagc gcacattatc acgggaaaga acgtgccgaa
     5821 atatcttaaa caggcgaaat ttaacgcatt gttgaagaag aaagacaaaa aaagccccac
     5881 cagaatggcg gggcaaagag aatagctagt tagctattta acgcgggacg ttcgctgata
     5941 gcgatacgct gcgctgcgat gggttcaggc tcattacgaa ttaaatcaat atgcagtaaa
     6001 ccgtttacga aggttgcgcc agagacttcc atattttcag ccagcgtaaa gctcaggcta
     6061 aatggctgat tcataagccc ttgatgcagc cattttttct cttcttttgg ctgctccggc
     6121 gtgcctttta cgctcaggcg cgtaccttcc agttgaatct ctaaatcttc ctgacggaaa
     6181 cctgccagcg caagggtaat gcggtagtgg ttatcgtcgc ttttctcaat gttgtacggc
     6241 gggaagctct ggctttcacc ggcgttttgc agtgcgttgg ccagtttgtc aaaaccgatc
     6301 cattgacgca tcagtgggga taaatcgaag ttacgcatag tcatttctcc ttctaagaag
     6361 cgagtaagta cctgcaaatc cgaagattcg catatgctcc ctgacggcga gcatggagat
     6421 gtcaggccgc gccaggcggc cttaaggaat tagttgattt cgatacggcg cggttttttc
     6481 gcttccggaa tcacgcgttc gagatcgata tacagcaaac catttaccag gttagcgcca
     6541 cgaacatgaa tgttctcagc taactggaat ttgcgttcaa agttgcgttc agcgatgccc
     6601 tggtacagat aggtgcgctc tttttgttcg tcggcgtgag cacctttcac caccagcaga
     6661 ttatcctggg cggtaatttc cagttcgctc tcagcaaaac cagccacagc gatagcaatg
     6721 cggtaatggt tttcgtctac cagttcaacg ttatacggag ggtagccgcc attactctgg
     6781 ctctggttgt tttctaagtg gttaaacaaa cggtcaaatc caatagcaga acggtaaagc
     6841 ggggataaat caaagttacg cataatcaat agctcctgaa atcagcgaga atgtaagacc
     6901 ttccacaatg gacaggtcag gtagccagaa cacccatcag gcgcgtcctc atcggctacg
     6961 atgtaaaaat gggtctggaa atgaactttt caagccttat cagcggactt ttttttgcag
     7021 tttatggtct attgcataga ctgagggggc agcacgaaac gttaaagtgc gatagccgcc
     7081 acagtgcgac gtaatgatga tgttattttc agcaagggat cgctataact catcaggcca
     7141 acacccacga tcaacagatg aatttattat gataagaaat gtgttgttag cgttcatgat
     7201 atgcagcgga atgacattac tcggaggatg ctccagcgtg atgtcccata ccggagggaa
     7261 agaaggaacg tatcccggca cccgcgccag cgcaaccatg attggcgacg atgaaaccaa
     7321 ctggggcact aagtcgctgg cgattctcga tatgccgttt accgctgtga tggacacgct
     7381 tttactgccg tgggatgtgt tccgcaaaga cagctcggtg cgctcacgcg ttgaaaaaag
     7441 cgaagccaac gcgcaagcca ccaatgcggt tattccaccg gccagaatgc ctgataactg
     7501 atttagcgat cagtttcggt gatccacagc tggccacctt ccatccacgc cagccattga
     7561 ccatccggcg agaagaccac ggcgtccgca gaaggtggat tggcgtggtt ttcggttaaa
     7621 tactcaacct cgccgctttg cgcatgggca caagcaattc gattatccag cacaaagccc
     7681 aaccattctc ctgacggatg ccagttaaat gcagactgaa tatccgtttt gttatgggtt
     7741 aactggcgcg gctcgccgcc ctgtggcgag ataagccaca gttgcacaat gccgttatca
     7801 tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac agcgcaccca gtggcgcggg
     7861 acgttgacta accccggata agcccgatgg tgggtaaagg ttaaacgtcg ctgcacgacg
     7921 ccgcgcggtg gcgcgggcag ggtggtttcc gttccactta acggcgcatc acctgccgct
     7981 ttccagccag cttcatcttg cggtaactca acgataaaca gctccggcac tttctcgccc
     8041 tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat ttcctaccca tccttcttca
     8101 taagcacgat tgatttcatc gctgccaggc tgcggcgtgg gcgtggtttt gctcaccagt
     8161 acgcaccagt ggctaccgct gtattcacgc ggatgctgct tttgtacgtt gaacgggcca
     8221 aacggtgcgg caacaccgac gtttcgcaaa tccagcgccg gatcgagttc atgcattaca
     8281 tggtcgttat aggtaaagct caccctttct ccgttcgggc taaagacatg cacatggctg
     8341 ccgccgcgca gcgctcctgg cgtatacggc gcggtaatat ccattgcatc gagattgctc
     8401 accttgccgc cttcagcaat gactccgcgt cgatgatgga agtcgtaatg ccatgtttca
     8461 tcaggatttt ccgggccatg gatgaaaaca tatttctctg acttcggatg aacggtcacc
     8521 acgccgacgt gtgcgccctg tgacgcgcga tagataacct cgacctcgcc ggtatggata
     8581 ttcacacgct caatggtctc gccggtaaac gacgccccag aaggacgcac gtcaaatacc
     8641 agccactggc tgtcgggcgt ccaggtattg gtattggtga gcaggtgatt acggggagca
     8701 aaggttatct gtttcatggc gcggtcctga tacaaaacgg gtcccaccat catacttcac
     8761 aaggacttca ctttcagaca atagtctttt gccatcgtga tgaatgtgag tgaaaagatg
     8821 gaacatttcg acgtggcgat tattggcctc ggcccggcag gatcggcgtt ggcgcgaaaa
     8881 ttagcaggca aaatgcaggt gatcgcgctg gataaaaagc accagtgtgg tactgaaggt
     8941 ttcagcaaac cttgcggcgg tctgctggca ccggacgcgc agcgatcttt tattcgcgat
     9001 ggactgacgc ttcctgtcga tgtgatcgcc aatccacaga ttttcagcgt caaaactgtc
     9061 gacgtcgccg catcgctcac gcgtaactac cagcgaagct atatcaatat taatcgccat
     9121 gctttcgact tgtggatgaa atcgctgatc cccaccagcg ttgaggttta tcacgatagc
     9181 ctgtgccgaa acatctggcg tgaggatgat aaatggcatg tcatttttcg tgcagacggc
     9241 tgggagcaac atattaccgc ccgctatctg gtcggtgccg atggcgcaaa ctcgatggtg
     9301 cggcgacatc tctacccgga tcatcaaatc cgtaaatatg tcgctatcca gcagtggttc
     9361 gcggagaaac atccggtgcc gttctactcc tgcatctttg ataattcgat aactaactgt
     9421 tattcatgga gtatcagcaa agacggttat tttatctttg gcggtgctta tccaatgaaa
     9481 gacggtcaga cgcgtttcac gacgctgaaa gagaaaatga gcgcctttca gttccagttt
     9541 ggtaaggcgg tgaaaagcga aaaatgcacg gtgctgtttc cctcacgctg gcaggatttt
     9601 gtctgcggta aggacaacgc ctttctgatt ggcgaagcgg caggatttat cagcgccagt
     9661 tcgctggagg ggattagcta tgcgctggat agcgcagaca ttctgcgtgc ggtgttactg
     9721 aagcagccag agaagctcaa taccgcttac tggcgcgcca cccgcaaact gcgtttaaaa
     9781 ctcttcggca agatagtaaa aagccgatgc ctgaccgcac cggctttaag aaagtggatt
     9841 atgcgcagtg gtatggcgca tattccacag ttgaaagatt agccaacgcg cttcacatcg
     9901 cccaccagca ggatgtaaga gagcgcgcca atcaacgcga cggcggagat ataaaccagt
     9961 gcaggtgcga aaccgtaacc ctgcgccagg taccccacca ccagcggaac ggtgatgccg
    10021 cccagaccac cggcgaagtt aaacacgccg ccggttaaac caatcaggcg catcggtgcc
    10081 agagaagaga ccagcgacca ggtaatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc
    10141 atcaggcaca taatcatcat cggatcgttg gtgtagttag cgcccataat gcaggtggag
    10201 atcagcaagc cgcagataat cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt
    10261 accagcagat ccgcgaccca gccagagagc aggacgccga caaacgccgc gaggaatggc
    10321 accgtggtca taaagcctgc tttcagcgcc gtgattcctt tttcctgggt taaatagttc
    10381 gggaaccagg ttaagaaaaa ccacagtgta gaagccaccg caaattgccc aagataaacg
    10441 ccgatcagtt tacgatggaa caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc
    10501 tctttcttca ccggcgcatc accatccacc agaccgccgc catcacgaat gtaatccagt
    10561 tcagctttgc tgataccttt ggtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc
    10621 gaccagataa tgccgatacc accagtgaca atgaacaccc agtgccagct caacatctcc
    10681 tgaatccaga tcagcagcgg agtcaaaaac gccagaccga caaactgacc agacgtataa
    10741 aaaccaacgg cggaagcgcg ttcatgttcc gggaaccagc tggtcaccat ccggttattg
    10801 gtcgggaaag cgggcgcttc gaaaataccg gttatcgcac gcagaccaat taatgacatt
    10861 aatcccgtgg caaagccctg gaataaagtc gccactgacc aaccaaatat cgcaataaaa
    10921 taagtcacgc gagaacctac gcgatctaaa aaccaaccgc cggggatctg gcatagcgta
    10981 taaagccagg cgaaggccga aaatacatag cccatttccg ctttcgtaat gccgaactct
    11041 tcctgaatat gggcggaagc cacggccagg ttagcgcggt caacataaca aatgaccacc
    11101 gtaataaaga tcatcaccag cgtcagataa cgccgacgcc ccggctttgc tgcattaacg
    11161 ggaatatcca tagcgagctt tctccagatt ttgggcatag cgaagccgct caccatgccc
    11221 tgtaatttac agagggttat atttttgtat tgctgtttta gtgcccgatg aggggcttac
    11281 gtggcaggaa ttaccactct gctacgctgt tatcttcatg acgccagagc ggattacgcc
    11341 agtccggggc atttttactg aactcaataa ctttagcttc gtcgatttcc acacctaagc
    11401 ccggtttcgt taacggttta aagaagccgc caaccatact gaagtcttct ttgtttttca
    11461 caaagtcgag taactccgcg cctttgttgt aatgaatgcc catactttgt tcctgaagta
    11521 ccgcgttata ggaaacaaag tcgatatgca ggcaagccgc cagtgcaatc ggtccgagcg
    11581 gacagtgcgg cgcaagggtc acatcatagg cttctgccat tccagcaatt ttgtagcatt
    11641 cggtaatacc gcctgcatgg gagagatccg gttgcagaat cgaaataccg cctgcctcca
    11701 gcacgcgttt aaaatcgaaa cgtgagaaca tgcgctcacc cgccgccagt ggaatatgcg
    11761 tttgtgccgc cagtttcggg tagtattcgg cttgttccgc cagcaccggc tcttcaataa
    11821 acagcgggcg atacggctcc agttctttaa tcagcacttt cgccattggc gcgctgacgc
    11881 gaccgtggaa atcaagacca aactcaatct gattgccaaa agcttcacga atttgtgcca
    11941 cggtgttgac tgccgcatct accgcgcggg agttatcaat tagccccagt tcttcacaac
    12001 cgttcagttt gaaggtatcg aagccgattt cgcgcagggt tttaatgccg tcgataacat
    12061 ccgccggacg atcgccgccg acccaactgt aggctttaat tttgtcgcga accaggccgc
    12121 ccatcagttg ccagaccggc gcattcagca ctttgccttt gatatcccat aacgcctggt
    12181 cgatcccggc aatggcgctc atcaggattg gaccgccacg ataaaatccg gcgcgataca
    12241 tcacttgcca taagtcattg atgcgcgaag gatcctgacc aatcaaatag tcacccagct
    12301 cgtgaactgc cgcttccacc gtacgggcgc ggccttcaat cacgggctcg ccccaaccga
    12361 ccacgccttc atcggtttca attttcagga acatccagcg gggaggtaaa cgatacgtgg
    12421 taattttggt gattttcatt gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg
    12481 ctgtgcggtg cgttctacgg actgcccggc gcgatagaga tcgctgccta agcccgcccc
    12541 agcacaacct gcgtctatcc actgcgccag gttttctggc gtcacgccgc caacggcaaa
    12601 gactgcgatg tccgatggca ataccgcttt taacgctttg atgtattgcg gaccaaaagc
    12661 cgatgacgga aatattttca gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt
    12721 cgccgtcgcg cagccggggc agacggtcat gccgtagccc accgcacggc ggatcacttc
    12781 actatggata ttgggcgtaa cgatgagctg acagcccatc ctggcgagcg catcgacctg
    12841 ttcaggtttc agtaccgtac ctgcgccaat caacgccttg tcgccatacg catcaacgat
    12901 ggcgggaatg ctttgctccc attgtgggga attcagcggg atttcaaccg cgtcgaaccc
    12961 ggcgtcaatc accgcgccaa catgcgccag cgcctcgtcg ggcttaatgc cgcgcaaaat
    13021 ggcgatcagc ggaagtttag tttgccactg catgagcgat gctccttata ccagcctgaa
    13081 atgccgtgtc gcccgccacc gccgtcacgt cgcaacccat cgcctgaaag gcttgctggt
    13141 agcgcgcggt cagcgatgtt ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt
    13201 cgcgcatact ggcaacctct gcgccaatca acaaaccaga gagaaattcg ctgacctgtt
    13261 cgcggggaag tgttcccagc acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg
    13321 cgggcgcatt aaggccgcgc tcaaggccag ccgcgaaggc atcggcagag ttttcctgcg
    13381 gcggcaaacc tgcgccaatc aatgagtgat ttaacagtaa atgatgtaat tcaccggtca
    13441 tcacggtgcg aaaatcgttg atttgctggc tatcggcctg cacccatttg caatgcgtac
    13501 cgggcataac ataaagagag gaaggagcca gagctcgcgc gccgatcaat tgtgtttctt
    13561 cgccgcgcat cacattgtgg ttatcgtcat gagagacgca taatccggga ataatccaga
    13621 tattgtcgcc aacagacgtt aattgttcgc caatagacga aaaacgggca ggaacagata
    13681 aatacggagc aactttccag ccgacattgc tgccgaccat tcctgccatc accactggcg
    13741 tattttcttc acgccagtcg gtcgtgactt ctgctaacac cgcagccgga gattttccgt
    13801 tcaggcgcgt gacgcctgct tctgattgcc tgctctccag gcagtggtcg ccctgataaa
    13861 gccaggcgcg cagattggtc gatccccagt caattgcgat gtagcgagct gtcatgtgat
    13921 ttcctttaac cttcgtgtcg agctggcgat catggtaagc gccgcctgct ctgccgcatc
    13981 gccgtcctga tgccgtattg catcgaacag cgccttatgt tcctggagcg tttgcggcat
    14041 gttggcctca tcgcccatcc aggttcgttc aaaaaccgcc cgctgcaacg aactgatcgc
    14101 aatgctaagt tgctgtaaca ccgggttatg taccgactgc agcaccgcct cgtggtagcg
    14161 aatatccgct tcgttaaacg cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc
    14221 cgattcaatc tgcgccagat cgctggaagt cgcgcgctct gctgcccaac gggcaatcgc
    14281 cggttccacc agatttcgca cttcgctcat ggcactgata agccgtgggt cgtagtcgtt
    14341 ttccaacacc cattgcagta cgtcagtgtc gaggtaattc cactggttac gcggtgccac
    14401 aaacgccccg cgataacgtt tcatttcaat cagccgcttc gccatcagcg aacggaacac
    14461 ctcacggatg atgttgcgcg aggttgcaaa ctcttcacag agctccgcct cagccggaag
    14521 tggcgagcca ggtacgtatt tgccgtgaac gatctgttta cccagcgtaa tgacaatgcg
    14581 atcggtttta ttgagagtca tggagagtcc ttgtgctctg tatgttcttc tctactttac
    14641 cccgatcgat gcataacgcg gcaactttgt agtaccagcg tgatgacgtt cgcgtttgcc
    14701 gtgtgcgtaa tgtagtacaa acttatattg ttgtactaca atttagatca caaaaagaac
    14761 aatgcataaa aaaatgacat gcgtcgggca gaaatctgaa aagggatatc aggcgctaaa
    14821 caggagggaa agaagagtat gctttcaacg gcttagctac tcgtttaaag gattaatcat
    14881 gaagttgaat tttaagggat tttttaaggc tgccggtttg tgttctttgg catttgcgtt
    14941 gaccggatgt atcaccgcag gactggtttt taataccgca agccctccac atgccgagca
    15001 atggcggtca gacacgatta agggtttatc gctggcagag gatagtaacg gtacgaaagg
    15061 ctatgttttt gttggtgaat cgttggatta tctgttgacc accggcggcg gtgaggtggt
    15121 taaaatgctg aacgatccgg caattcatgg tgaaagaatc accgtttctg ataacgcgaa
    15181 atttatttta agcagcagta ataagaattt ctctggtgct attacgctct attaccactg
    15241 gaacaacgaa gaagataagg cactggcaac acaatatggt tttatttgtg atacccgccg
    15301 ttgtacatgg atgctggacg gcctgaaagg ctctatccac cagaaaaata aaaaagcgga
    15361 ttactcaaac gtgatggttt tccatcaacc ctttacggtg ggattttatg agtacaaagc
    15421 atcagatggc gttccgcgcg gcctcgttaa tgcgctgcta ccagtcactc ttacgctgga
    15481 tatagtaacg tcaccactgc agttcctgat tttatgcact accagaaatt gttaatcgct
    15541 gcgggcaaac gcgtttatgc tgtttgcccg tccactgatt aattcagcac atacttctca
    15601 atagcaaacg ccacgccatc ttcaaggtta gatttggtga caaagttcgc cacttctttc
    15661 actgaaggaa tagcgttatc catcgccaca ccgacgcctg catattcaat cattgcgata
    15721 tcgttttcct gatcgccaat cgccatgatt tcttccggtt taatacctaa cacgtcggcc
    15781 agtgatttca cccccgtacc tttgttaacg cgtttatcga ggatttcgag gaagtacggc
    15841 gcacttttca gcacggtata tttctctttc acttcctgcg gaatacgcga gatagcctgg
    15901 tcgaggatgg cgggttcatc aatcatcatc actttcagga actgggtatt ggggtccatt
    15961 ttctccgctt cgcagaacac cagcggaatg gtggcaacga aggattcatg caccgtgtag
    16021 tagctgatat cacggttggc ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa
    16081 ccgacttcgc gagagagttt ttccaggaaa cgatagtcgt catagctgag agcagtttgc
    16141 gccacggtgc taccatcagc ggccttctgt accagcgcgc cgttataagt aatgcagtag
    16201 tcgcccggct gttccatatg cagctctttc aggtagttgt gcacacctgc atacgggcga
    16261 cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg cggcaatcgc atttttaacg
    16321 gcgggtgaaa tggtgtgatc gggcagcaga agggtgccat ccatatcgat agcaatgagt
    16381 ttgatagcca tgagttcccc aggtagatta gttcctgacc catgctaacg cgattccgct
    16441 caaaaatcag tacaacaccc gagggaaaag ggggatgcaa cgcgggttgc atgctcccca
    16501 tttgcttagc ggaagagttt ccctttcagc agttccatac ctgcggaaag cagatcgtta
    16561 ttggcttgtg gtgacacttc accttgcggt gagagcgcat caataatctt cggcaattgc
    16621 tctgccagta aactggaagc tgtactggta tccacgccaa gtttttgccc gagatcggac
    16681 accgcatttg tgccgagcgc cgattccagt tgctcgccac taaccgattg attgccctgt
    16741 tgattactca gccaggttga gagaatggcc cctaagccgc cactttgcag tttttccagc
    16801 agcacctgaa tgccgccctg ctcctcaacc caacttaaaa tagcctgata tttccccgca
    16861 tcgcctttca gaaaggcacc gacaacttca tcaaaaagcc ccatgataat cacctgtaaa
    16921 gcgttacgtg ttgacccaaa aagtatagat ttgcgggatg ataattgcgg attgcagaaa
    16981 taaaaagggc ggagatgatc tccgcccttt tcttatagct tcttgccgga tgcggcgtga
    17041 acgccttatc cggcctacaa aatcatgaaa attcaataca ttgcaagatt ttcgtaggcc
    17101 tgataagcgt agcgcatcag gcacgctcgc atggttagcg ccattaaata tcgatattcg
    17161 ccgctttcag ggcgttctct tcaataaacg cacggcgcgg ttcaacggcg tcgcccatca
    17221 gcgtggtgaa caactggtcg gcagcaatcg catctttaac ggtaacgcgc agcatacgac
    17281 ggctttccgg gtccatggtg gtttcccaca gctgttccgg gttcatctcg cccagacctt
    17341 tataacgctg gatggagagg ccgcgacggg actctttcac cagccagtcc agcgcctgct
    17401 cgaagctggc taccggctga cgacgctcgc cacgttcgat aaacgcatct tcttccagca
    17461 agccacgcag tttctcaccc agcgtgcaga tacgacgata ttcgccaccg gtgataaact
    17521 cgtgatccag cggatagtca gtatccacac cgtgggtacg cacgcgaaca atcggttcaa
    17581 acaggttttg ttcggcattg gtgtggacat caaacttcca ctggctgccg tgctgttctt
    17641 tgtcgttcag ttcgctgacc agcgcgttca cccagcgggt aacggtctgc tcatcagaga
    17701 ggtcggcttc cgtcagcgtc ggctgataga taagctcttt cagcattgct ttcggataac
    17761 gacgctccat gcggttgatc attttctgcg tcgcgttgta ctcagacacc aatttctcta
    17821 acgcttcgcc agccaatgcc ggtgcactgg cgttggtgtg cagcgttgcg ccatccagcg
    17881 cgatggagat ttggtactga tccatcgctt cgtcgtcttt aatatactgt tcctgcttgc
    17941 ctttcttcac tttgtacagc ggcggctgag cgatgtagac gtgaccgcgt tcaacgattt
    18001 ccggcatctg acgatagaag aaggtcaaca gcagcgtacg aatgtgcgag ccgtcgacgt
    18061 ccgcatcggt catgatgatg atgctgtgat aacgcagttt gtccgggttg tactcgtcac
    18121 gaccgatacc acagccaagc gcggtgataa gcgtcgccac ttcctgagaa gagagcatct
    18181 tatcgaagcg cgctttctca acgttgagga ttttaccctt cagcggcaga atcgcctggt
    18241 tcttgcggtt acgcccctgc ttcgcagagc cgcccgcgga gtccccttcc accaggtaca
    18301 gttcggaaag cgccggatcg cgttcctggc agtctgccag tttgcccggc aggcccgcta
    18361 agtcgagcgc acctttacgg cgggtcattt cacgcgcgcg acgcgccgct tcacgggcac
    18421 gggcagcatc gataattttg ccaaccacga ttttcgcgtc ggttgggttt tccagcaggt
    18481 attctgccag cagttcgttc atctgctgtt caaccgccga tttcacctca gaagaaacca
    18541 gtttgtcttt ggtctgggag gagaatttcg ggtccggcac tttcacggaa acgaccgcaa
    18601 tcaggccttc acgcgcatcg tcaccggtgg cgctgacttt ggcttttttg ctgtagcctt
    18661 ctttgtccat gtaggcgttc agggtacggg tcatcgccgc acggaagcct gccaggtgag
    18721 taccgccgtc acgctgcgga atgttgttgg taaagcagta gatgttttcc tggaagccat
    18781 cgttccactg caacgccact tcgacgccaa taccgtcttt ttcggtggag aagtagaaga
    18841 tatttgggtg gatcggcgtt ttgttcttgt tcagatattc aacgaacgcc ttgatgccgc
    18901 cttcatagtg gaagtggtct tctttgccgt cacgcttgtc gcgcagacga atggaaacgc
    18961 cggagttgag gaacgacaac tcacgcagac gtttcgccag aatttcatat tcgaactcgg
    19021 tcacattggt gaaggtttcg aggctaggcc agaaacgcac catggtgccg gttttttcag
    19081 tctcgccggt aaccgccagc ggggcctgcg gtacaccgtg ttcgtagatc tgacggtgaa
    19141 ttttaccctc gcgctggata accagctcca gtttttgcga cagggcgttt actaccgaaa
    19201 caccaacgcc gtgcagaccg ccggacactt tataggagtt atcgtcaaat ttaccgcctg
    19261 cgtgcagaac ggtcatgatc acttccgccg ccgatacgcc ctcttccggg tgaataccgg
    19321 tcggaatgcc gcgcccgtcg tcctgtacag agacagagtt gtcggcgtga atggtgacga
    19381 taatttcttt acagtgaccc gcgagcgctt cgtcgatagc gttatctacc acctcgaata
    19441 ccatgtggtg cagaccggtg ccgtcatccg tgtcgccgat atacataccc gggcgcttac
    19501 gcaccgcatc cagccctttc aggactttga tactggagga gtcataagaa ttcgacatca
    19561 acgtttctcg ctcatttata cttgggttaa tccgttattt tacccttttc cacggtaaac
    19621 atcttcgaat tttcgtccga catgtctata acgtgttcag cactgatcgc gctgacaaag
    19681 acctgtgatt gcgtcgcttt taagcggctg gcaagcagcc cgcgacgctc atcatcaagc
    19741 tcagaggcaa aatcatctat caggtagaga caccgccgcc cgctttcacg ggtgaggaac
    19801 tctccttgcg ccagacgtaa ggcgcacatc aacagcttaa gctgcccacg cgataaggta
    19861 tcttccaccg gcgcaccgtc ggcgcgaatg cgtaagtccg ctttatgcgg gccatgcgcg
    19921 gtgtaggtta gctggcgatc gcgttcaaaa ttacgttcca gcacctcagc atattctgtc
    19981 tctttctccc agccgcgctg gaaagagaaa gtcaga
//
LOCUS       DAEVZA010000093        19525 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEVZA010000093 DAEVZA010000000
VERSION     DAEVZA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.093"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            42..2420
                     /gene="pgaA"
                     /locus_tag="LAF92_003384"
     CDS             42..2420
                     /gene="pgaA"
                     /locus_tag="LAF92_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HBI9792338.1"
                     /translation="MKLLTAAFLAASPAAKSAVNNAYDALIIEARKGNTQPALSWFAL
                     KSALSNNQIADWLQIALWAGQDKQVITIYNRYRHQQLPARGYAAVAVAYRNLQQWQNS
                     LTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNSGAPDKANLLAEAYIY
                     KLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHA
                     ELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTR
                     DRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQSIMTELFYHKETIAPD
                     LSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTWLQGHSFL
                     STVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIE
                     PRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIA
                     GSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADGQFSEGKGIVRDWLAG
                     VEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMK
                     NGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDF
                     LPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKH
                     YGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVEFDMTFRF"
     gene            2429..4447
                     /gene="pgaB"
                     /locus_tag="LAF92_003385"
     CDS             2429..4447
                     /gene="pgaB"
                     /locus_tag="LAF92_003385"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBI9792339.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4440..5765
                     /gene="pgaC"
                     /locus_tag="LAF92_003386"
     CDS             4440..5765
                     /gene="pgaC"
                     /locus_tag="LAF92_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HBI9792340.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5767..6180
                     /gene="pgaD"
                     /locus_tag="LAF92_003387"
     CDS             5767..6180
                     /gene="pgaD"
                     /locus_tag="LAF92_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HBI9792341.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6230..7018)
                     /gene="phoH"
                     /locus_tag="LAF92_003388"
     CDS             complement(6230..7018)
                     /gene="phoH"
                     /locus_tag="LAF92_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBI9792342.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7637..8908)
                     /gene="efeB"
                     /locus_tag="LAF92_003389"
     CDS             complement(7637..8908)
                     /gene="efeB"
                     /locus_tag="LAF92_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBI9792343.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(8914..10041)
                     /gene="efeO"
                     /locus_tag="LAF92_003390"
     CDS             complement(8914..10041)
                     /gene="efeO"
                     /locus_tag="LAF92_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBI9792344.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10099..10929)
                     /locus_tag="LAF92_003391"
     CDS             complement(10099..10929)
                     /locus_tag="LAF92_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBI9792345.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11595..13103)
                     /gene="putP"
                     /locus_tag="LAF92_003392"
     CDS             complement(11595..13103)
                     /gene="putP"
                     /locus_tag="LAF92_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBI9792346.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSSI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFMRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13262..13471)
                     /locus_tag="LAF92_003393"
     CDS             complement(13262..13471)
                     /locus_tag="LAF92_003393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792347.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13526..17488
                     /gene="putA"
                     /locus_tag="LAF92_003394"
     CDS             13526..17488
                     /gene="putA"
                     /locus_tag="LAF92_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBI9792348.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17528..18166)
                     /gene="rutR"
                     /locus_tag="LAF92_003395"
     CDS             complement(17528..18166)
                     /gene="rutR"
                     /locus_tag="LAF92_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBI9792349.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            18454..>19525
                     /gene="rutA"
                     /locus_tag="LAF92_003396"
     CDS             18454..>19525
                     /gene="rutA"
                     /locus_tag="LAF92_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBI9792350.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPA"
BASE COUNT         4915 a         5092 c         4846 g         4672 t
ORIGIN      
        1 attcaagtag cagaaaaagg tgcccgaaaa ccaaatgggc tttgaaactt cttactgccg
       61 catttttagc agcgagtccc gcggcgaaga gtgctgttaa taacgcctat gatgcattga
      121 ttattgaagc tcgcaagggt aatactcagc cagctttgtc atggtttgca ctaaaatcag
      181 cactcagcaa taaccaaatt gctgactggt tacagattgc cttatgggcc gggcaagata
      241 aacaggttat taccatttac aaccgctacc gtcatcagca attaccagcg cgtggttatg
      301 cagctgtcgc cgtcgcttat cgtaacctgc aacaatggca aaactcgctt acactgtggc
      361 aaaaggcgct ctctctggag ccgcaaaata aggattatca acggggacaa attttaaccc
      421 tggcagatgc tggtcactat gatactgcgc tggttaaact taagcagctt aactctggag
      481 caccggacaa agccaattta ctcgcagaag cctatatcta taaactggcg gggcgtcatc
      541 aggatgaatt acgggcgatg acagagtcat tacctgaaaa tgcatctacg caacaatatc
      601 ccacagaata cgtgcaggca ttacgtaata atcaacttgc tgccgcgatt gacgatgcca
      661 atttaacgcc agatattcgc gctgatattc atgccgaact ggtcagactg tcgtttatgc
      721 ctacgcgcag tgaaagtgaa cgttatgcca ttgccgatcg cgccctcgcc caatacgctg
      781 cattagaaat tctgtggcac gataacccag accgtactgc ccagtaccag cgtattcagg
      841 ttgatcatct tggcgcgtta ttaactcgcg atcgttataa agacgttatt tctcactatc
      901 agcgattaaa aaagacgggg caaattattc cgccctgggg gcaatattgg gttgcatcgg
      961 cttatctcaa agatcatcag ccgaaaaaag cacagtcaat aatgaccgag ctcttttatc
     1021 acaaggagac cattgccccg gatttatccg atgaagaact tgcggatctc ttttacagcc
     1081 acctggagag tgaaaattat ccgggcgcgc taactgtcac ccaacatacc attaatactt
     1141 cgccgccttt ccttcggtta atgggcacgc ctacgagcat cccgaatgat acctggttac
     1201 aggggcattc gtttctctca accgtagcaa aatatagtaa tgatcttcct caggctgaaa
     1261 tgacagccag agagcttgct tataacgcac caggaaatca gggactgcgc attgattacg
     1321 cgagtgtgtt acaagcccgc ggttggcctc gtgcagcaga aaatgaatta aaaaaagcag
     1381 aagtgatcga gccacgtaat attaatctgg aggttgaaca agcctggaca gcattaacgt
     1441 tacaagaatg gcagcaggca gctgtcttaa cgcacgatgt tgtcgaacgt gaaccgcaag
     1501 atcccggcgt tgtacgatta aaacgtgcgg ttgatgtaca taatcttgca gagcttcgta
     1561 tcgctggctc aacaggaatt gatgccgaag gcccggatag tggtaaacat gatgtcgact
     1621 taaccaccat cgtttattca ccaccgctga aggataactg gcgcggtttt gctggattcg
     1681 gttatgccga tggacaattt agcgaaggaa aagggattgt tcgcgactgg cttgcgggtg
     1741 ttgagtggcg gtcacgtaat atctggctcg aggcagagta cgctgaacgc gttttcaatc
     1801 atgagcataa acccggcgcg cgcctgtctg gctggtatga ttttaatgat aactggcgta
     1861 ttggttcgca actggaacgc ctctctcacc gcgttccatt acgggcaatg aaaaatggtg
     1921 ttacaggcaa cagtgctcag gcttatgttc gctggtatca aaatgagcgg cgtaagtacg
     1981 gtgtctcctg ggctttcact gatttttccg acagtaacca gcgtcatgaa gtctcacttg
     2041 agggtcagga acgcatctgg tcttcaccat atttgattgt cgatttccta cccagtctgt
     2101 attacgaaca aaatacagaa cacgataccc catactacaa ccctataaaa acgttcgata
     2161 ttgttccggc atttgaggca agccatttgt tatggcgaag ctatgaaaat agctgggagc
     2221 aaatattcag cgcaggtgtt ggtgcctcct ggcaaaaaca ttatggcacg gatgtcgtca
     2281 cccaactcgg ctacgggcaa cgcattagct ggaatgacgt gattgatgct ggcgcaacgc
     2341 tacgctggga aaaacgacct tatgacggtg acagagaaca caacttatac gttgaattcg
     2401 atatgacatt cagattttaa ggataaatat gttacgtaat ggaaataaat atctcctgat
     2461 gctggtgagt ataattatgc tcaccgcgtg cattagccag tcaagaacat catttatacc
     2521 gccacaggat cgcgaatctt tactcgccga gcaaccgtgg ccgcataatg gttttgtagc
     2581 gatttcatgg cataacgttg aagacgaagc tgccgaccag cgttttatgt cagtgcggac
     2641 atcagcactg cgtgaacaat ttgcctggct gcgcgagaac ggttatcaac cggtcagtat
     2701 tgctcaaatt cgtgaagcac atcgaggagg aaaaccgcta ccggaaaaag ctgtagtgct
     2761 gacttttgat gacggctacc agagttttta tacccgcgtc ttcccaattc ttcaggcctt
     2821 ccagtggcct gctgtatggg cccccgtcgg cagttgggtc gatacgccag cggataaaca
     2881 agtaaaattt ggcgatgagt tggtcgatcg agaatatttt gccacgtggc aacaagtgcg
     2941 agaagttgcg cgttcccggc tcgttgagct cgcttctcat acatggaatt ctcactacgg
     3001 tattcaggct aatgccaccg gcagcttatt gcctgtatat gtaaatcgtg catattttac
     3061 tgaccacgca cggtatgaaa ccgcagcaga ataccgggaa agaattcgtc tggatgctgt
     3121 aaaaatgacg gaatacctgc gtacaaaggt tgaggtaaat ccacacgttt ttgtttggcc
     3181 ttatggcgaa gcgaatggca tagcgataga ggaattaaaa aaactcggtt atgacatgtt
     3241 cttcaccctt gaatcaggtt tggcaaatgc gtcgcaattg gattccattc cgcgggtatt
     3301 aatcgccaat aatccctcat taaaagagtt tgcccagcaa attattaccg tacaggaaaa
     3361 atcaccacaa cggataatgc atatcgatct tgattacgtt tatgacgaaa acctccagca
     3421 aatggatcgc aatattgatg tgctaattca gcgggtgaaa gatatgcaaa tatcaaccgt
     3481 gtatttgcag gcatttgctg atcccgatgg tgatgggctg gtcaaagagg tctggtttcc
     3541 aaatcgtttg ctaccaatga aagcagatat ttttagtcgg gttgcctggc aattacgtac
     3601 ccgctcaggt gtaaacatct atgcgtggat gccggtatta agctgggatt tagatcccac
     3661 attaacgcga gtaaaatact taccaacagg ggagaaaaaa gcacaaattc atcctgaaca
     3721 atatcaccgt ctctctcctt tcgatgacag agtcagagca caagttggca tgttatatga
     3781 agatcttgcc ggacatgctg cttttgatgg catattgttc cacgatgatg ctttgctttc
     3841 agattatgaa gatgccagtg caccggctat cacggcttat cagcaagcag gctttagcgg
     3901 gagtctgagc gaaattcgac aaaacccgga gcaatttaaa cagtgggccc gctttaaaag
     3961 tcgtgcgtta actgacttca ctttagaact tagtgcgcgc gtaaaagcca ttcgcggtcc
     4021 acatattaaa actgcacgaa atatttttgc acttccggta atacaacctg aaagtgaagc
     4081 ctggtttgca cagaattatg ctgatttcct aaaaagctat gactggaccg ctattatggc
     4141 tatgccttat ctggaaggtg tcgcagaaaa atcggctgac caatggttaa tacaattgac
     4201 caatcaaatt aaaaacatcc ctcaggctaa agacaaatct attttagaat tacaggcaca
     4261 aaactggcag aaaaatggtc agcatcaggc tatttcttcg caacaactcg ctcactggat
     4321 gagcctatta caactgaatg gagtgaaaaa ctatggttat tatcccgaca attttctgca
     4381 taaccaacct gaaatagacc ttattcgtcc tgagttttca acagcctggt atccgaaaaa
     4441 tgattaatcg catcgtatcg ttttttatat tatgtctggt gttatgcata cccctatgcg
     4501 tagcgtactt tcactctggt gaactgatga tgaggttcgt tttcttctgg ccgtttttta
     4561 tgtccattat gtggattgtt ggcggcgtct atttctgggt ctatcgtgaa cgccactggc
     4621 cgtggggaga aaacgcacca gctccccagt tgaaagataa tccgtctatc tccattatca
     4681 ttccctgttt taatgaggag aaaaacgttg aggaaaccat acacgccgct ttagcacagc
     4741 gttatgagaa cattgaagtt attgccgtaa atgacggttc aacagataaa acccgtgcca
     4801 tcctggatcg catggctgca caaattcccc atttgcgggt cattcatctg gcgcaaaacc
     4861 aggggaaagc cattgcgctt aaaaccggag ctgccgcggc gaaaagtgaa tatctggtgt
     4921 gcattgatgg cgatgcgtta ttagaccgcg atgcggcggc atatattgtg gaaccgatgt
     4981 tgtacaaccc gcgtgtgggt gccgtaaccg gtaatcctcg tattcgaaca cgttctaccc
     5041 tggtgggtaa aattcaggtt ggcgagtatt cctcaattat tggtttgatc aagcgaaccc
     5101 agcgtatcta tggaaacgta tttaccgttt ccggtgttat tgccgcattt cgtcgcagcg
     5161 ccctggcaga agtgggttac tggagtgacg atatgatcac cgaagatatt gatattagct
     5221 ggaagctgca gttgaatcag tggacgattt tttacgagcc acgggcactg tgctggatat
     5281 taatgcctga aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg
     5341 cagaagtatt cctcaaaaat atgacaaggc tatggcgcaa agaaaacttt cgaatgtggc
     5401 cactgttttt tgaatactgt ctgacgacaa tatgggcctt tacctgcctg gtcggtttca
     5461 ttatttacgc agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg
     5521 cgacacatac tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc
     5581 tgatgatcga gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt
     5641 tcccggttat tttctggatg ctgagcctgg caacgacatt ggtatcattt acacgagtca
     5701 tgttgatgcc taaaaagcaa cgcgcccgtt gggtaagtcc cgatcgcggg attctgagag
     5761 gttaatatga acaatttaat tattacgacc cgacaatcgc cagtacgttt actggttgat
     5821 tatgttgcca caaccatctt gtggacatta tttgctttgt tcatattctt attcgccatg
     5881 gatctgctga cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt
     5941 ttgctggcag tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctgcgt
     6001 tttcaaaaac agcagcatca tgcagcctac caatatacgc cgcaagaata tgcagagagc
     6061 ttagcaatac ctgatgagct ctatcagcaa ctacaaaaaa gccataggat gagcgtacac
     6121 ttcaccagcc aggggcaaat aaaaatggtt gtttcagaaa aagcgctagt ccgggcataa
     6181 acacccaaaa caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg
     6241 catgcagcgt acgttggcaa agtgccgaac gtacgcagtc ctctttaccg aaccggacga
     6301 tcccaaccat ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcacacgc
     6361 cgcgaggcaa atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga
     6421 ggcgggttaa aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga
     6481 cgactgcatt ttcaaaggta cgtccacgca tataggcgaa cggcgcaatt tccaccttcc
     6541 ctatttccgg tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcacgt
     6601 cgtagaccgg gcgaaaatag ggagcaaact tttctgcgat atctccaggt aagaagccaa
     6661 gatcttcatc ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta
     6721 tcagggcctc tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg
     6781 tggcgaatat cagctgctta ctctcaatag ccttcagata gtgcaattgc gcttcatttc
     6841 gcgcgaggat gggcgaagta tcgcgactgt cgcgggccat accaatggct tctacgccgc
     6901 ccatctgcac aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc
     6961 gtctcagcac acgttttgcc tcacgacgag ctttgatcac tgctttttgt cttcccatgg
     7021 agagcacctt gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac
     7081 gaacatcaga gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa
     7141 aacggctacg cacaccgttt gcggcgtcgg taacacgagt aaagagagga agtgagaaaa
     7201 atacagagtg acgaagttgc taccggagga gaaatctccg caagtggtgg agcgtagctt
     7261 aacaagacat tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc
     7321 actgccggga acttccgctg ctacttaata attacaacag atctcgcatt cattgcaaca
     7381 atatattcac ttatacttaa ccataaaaca ccatttcagt gacattagtt tctactggaa
     7441 agatgacaga gtgatgacag atatgaaaaa agctgtgtgc tttcagcagg attcgcatca
     7501 ggcatctgcg cacgaacgcc ggatgcggcg tgaacgcctt atccgcctac cgaacccgca
     7561 ccgattgtag gcataagaag acgcgcaagc atcgcatcag gcatctgcgc aagaacgcct
     7621 tatccgtcta taaacattaa acccgcaata acgcgcttcc gagataatcg ttcgcgtcct
     7681 tcactcccgg cagcgcaaaa aaataaccgc cgccgatagg tttaacgtat tcctccagcg
     7741 cttcgccatt gagccttttt tgtacagtca ggaagccttt ttccagatcg tgttggtaac
     7801 agacaaacag tagccccata tccagttgcc cggagttggt gacgcccagt gaataactgt
     7861 agccacgacg cagcatcagg ctggactcac tctccgccgt gcggggattc gccagccgga
     7921 tatggctgtc cagcgcaatc accttccctt ccggatcgct ggcgtaatcg ggaacatcat
     7981 gctcatgctg cattcccagc ggcgcaccgg tttgcttatc acgaccaaaa atcgtctgct
     8041 gttctttcag cggagttctg tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct
     8101 gatagctgcc accgattgtc cacgctggct cctgctgatc tgccgttacc cacaccactt
     8161 tttgcatcaa cttatcattc tggctatcgg gattggcagt gccgtctttg aaacccagca
     8221 aattaatcgg cgtctctttg cctttactac gcgccgcgtg atcggaaata aacccttccc
     8281 gcttccagcg cacgctgagc aaatctggcg tatgtttgat gatatcgcgc agcgcatgga
     8341 tcaccgtgtc ctgggtgttg gcgcaaattt gtagcaacac atcaccatga cataacgccg
     8401 catccagcga gtcgttggga aaacgcgtca tcttctgcaa ctttttcggc atctgtggcg
     8461 caaggccaaa gcgctcatca aacaatgagt gacccaccga taacgtgatg gtgagattat
     8521 cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc ggatttggcg
     8581 tttctggtgc cgctccgccc tgagtcagaa aagcaaaacg ctgagtcaac aagcgaaaca
     8641 accgttcaag atcgggttta tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg
     8701 cctgttgtgg cgtcaggatc cctgcctgat gctcaccata aaacggctgt ttctcattgc
     8761 gtgcatccgg tgaaagcgta cccggcgcac tttgcgtttt ttgtgcatga gcgaccggac
     8821 aacttcccgc cagcgccagc gcaccaatgc ctttcagtaa acgtcggcgt gacggttcat
     8881 tcacgccgtt tttatcttca tactgcataa tgcttaatcc aatcccagca caccgcgaag
     8941 ttgcgccaga tcttccgcca gcgcagtaat cggtcctttc agtgcattcc ggtcggcatc
     9001 ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca gaatggtatc
     9061 gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt tttgcagttg
     9121 tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga aatcccacag
     9181 atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca cttcctcaat
     9241 cagtccggct gcaccgccga ccacttttga aggtgggaaa gccagttcac tgatgcgttt
     9301 ttgcaaatcg accacatcgg tataaagctg gtcagcgtac tgatccatgc ctttggtggt
     9361 gttgtcgcca aacaatgctt tttccagacg gtggaaaccg gtgaatttcg gatcggcggc
     9421 tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg agaacagttc
     9481 agcaatcggt tcgatgcgct catagtgctg gcgcgtcggc gcatacagtg ctttcgcttt
     9541 ttcgatatcg cctgctttaa tagcgtcagt aaaggctttc gtgtcggtca ccagctgcgt
     9601 ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac ttaacagcgc
     9661 atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc ctttcgggtt
     9721 agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg tcattttctg
     9781 gctaaagcca ggggcgatat tttcccgctc ttccaccacc atcacgcctt tgaggatctc
     9841 ccactccagc gccttctggc tgtggttctg aataatgaac tgtgttttcc cggcgttaac
     9901 cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct gcgtcacatc
     9961 agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact gcaatgcgtt
    10021 acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt aactaagcaa
    10081 gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc gtggtggcag
    10141 agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt cgctgacgct
    10201 cggcgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg agtgagttga
    10261 gagcaccgca ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc cggcttcatg
    10321 aaatgcgcga atggcaccag ctgccagccc tgcggcgacg aagagaataa acaggctggt
    10381 ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca ggaagcctag
    10441 caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc cgacatcttg
    10501 ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa cggcaaaaaa
    10561 gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc tatcgactgc
    10621 ctgctccagt tgtactttga cgttgcgcga cactttgcgc atccagaaaa ccatccaggt
    10681 aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgtt ctttttgcgg
    10741 aaattcgccg gtggtttcgt taatgaagat acccaggccc aggcacaacg cagcggcaag
    10801 caacacgcca atccacatca caccaatcca tcggcctcgc tgggtacgct taagatagct
    10861 ggcaatcaaa ctgacaatca gcgcggcttc aagtccttcg cgcaacataa tgagaaacgg
    10921 aacaaacatg ccacccaccc ttaaacgttt ttctgagtca atctatgcaa agaaaagtaa
    10981 atcgcagtga tagcgattat cattacggca aagaaaaata caagcggaat gtttataaaa
    11041 tgatgttgga atgtaaacaa agagaggtaa tgccaaggga tataaacgat tattccaggt
    11101 acaaagatgg gattgtttac aacgagccat gtgttcttgc cggatgccgc gtaaacgccg
    11161 tatccagccg acaaaaccgt cctgactcaa tacatcaaaa cttccatgta aaactgataa
    11221 gcgggtgtat caagcaattt cgtccatcct cgattatcaa atctaacttt ttgcccggaa
    11281 cataagaaca gcgcgggtgt tggtggcgtc aaaatcgctg aagcgttccc cgaaggccgg
    11341 ggcagcccac atggatgtgg gctgagggcg cgtcttacag gacgttacct cgcgcccgac
    11401 ccggtagccg aaggggataa gctgaaggta ccgcgcagcg gcgattttgt tcgccagagc
    11461 ccgggggtgc agggggccgc ggcgattggc cgccccctgc gcgctccctg caccattcaa
    11521 tttatatggc gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc
    11581 gcaggctaag tcccttagct ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca
    11641 tcggcctcgg caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac
    11701 actacaatcc caatactgcc gaagataaag cccggaataa tttcgtacag tcccagccag
    11761 ccgaactgtt tccagacgat aaccgtcagc gccccgatga tcatccccgc cagcgcaccg
    11821 ttgcgcgtca tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca
    11881 aagcctgccc acgcatagct cactagcccc agcacacggt tttccgggtt tgccgccagc
    11941 gcaatggcca ccagcgccac caccagcacc atcacacgcc ctacccacac cagctctttc
    12001 tggctggcat gtttacgcat aaacgctttg tataaatctt cggtaatcgc actggagcac
    12061 accagcagct ggcaacttaa ggttgacatt accgccgcca gaattgacga cagcagaatc
    12121 ccggcaatcc acgggttaaa cagaatttgc gccagttcga taaacacacg ttcagcattc
    12181 tggtttaccg cacctgctac cgccgggtgc tcattaaagt aagcaatacc aaagaagccg
    12241 acagctaccg cccctgccag gcagaggatc atccaggtca tactgatacg acgcgcatgg
    12301 acaatgctgt ggtgagagtc tgccgccata aaacgcgcca ggatgtgagg ctgcccgaag
    12361 taacccagcc cccagcccat caatgagata atggcgacaa agttcagccc tttgagcata
    12421 tcaacgtttt cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca
    12481 ctgataatga cgataaccgg tgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca
    12541 gtatcagtcc agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgccgca
    12601 gctcccgccc acagagccgt ttcgtagctc atgccaaagg tactttcaaa cagacgcgcg
    12661 cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag
    12721 ataatgcgca aaatgcggct tttatcttca aagcgcccgg tgaaataatc cggcagggtt
    12781 aaggcgttat tgttgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg
    12841 atccacgcgc ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa
    12901 accgcgcccg gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat
    12961 gccgtcacga atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt
    13021 gatcgccagg cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc
    13081 aacatcggtg tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa
    13141 acttgccgtt atatctgcca ccggaacggg gtaacagagt ttatgtttta ccagggcgac
    13201 cgtatcctgc cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat
    13261 tttagtgctc agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg
    13321 cacctttcaa aaatgttaac tgccgcagag aaaaagtctg agttattttt ttaatccctg
    13381 tcatatcgat ttcttttatt aacatttcat tcatttttaa gcttgctacg caggtcacat
    13441 ttaacatagt tgcacaaagt tgcaacatca tggatatttc acgataacgt taagttgcac
    13501 ctttcagaac aacaggagta atggcatggg aaccaccacc atgggggtta agctggacga
    13561 cgcgacgcgt gagcgtatta agtctgccgc gacacgtatc gatcgcacac cacactggtt
    13621 aattaagcag gcgatttttt cttatctcga acaactggaa aacagcgata ctctgccaga
    13681 gctacctgcg ctgctttctg gcgctgccaa tgagagcgat gaagcaccga ctccggcaga
    13741 ggaaccacac cagccattcc tcgactttgc cgagcaaatt ttgccccagt cggtttcccg
    13801 cgccgcgatc accgcggcct atcgtcgccc ggaaaccgaa gcggtttcta tgctgctgga
    13861 acaagcccgc ctgccgcagc cagttgctga acaggcgcac aaactggcgt atcaactggc
    13921 cgataaactg cgtaatcaaa aaaatgccag tggtcgcgca ggtatggtcc aggggttatt
    13981 gcaggagttt tcgctgtcat cgcaggaagg cgtggcgctg atgtgtctgg cagaagcgtt
    14041 gttgcgtatt cccgacaaag ccacccgcga cgcgttaatt cgcgacaaaa tcagcaacgg
    14101 taactggcag tcacacattg gtcgtagccc gtcactgttt gttaatgccg ccacctgggg
    14161 gctgctgttt actggcaaac tggtttccac ccataacgaa gccatcctct cccgctcgct
    14221 gaaccgcatt atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg atatggcgat
    14281 gcgcctgatg ggcgagcagt tcgtcacggg tgaaaccatc gcggaagcgt tagccaatgc
    14341 ccgcaagctg gaagagaaag gtttccgtta ctcttacgat atgctgggcg aagctgcact
    14401 gactgccgca gacgcacagg cgtatatggt ttcctatcag caggcgattc acgccatcgg
    14461 taaagcgtct aacggtcgtg gcatctatga agggccgggc atttcaatca aactgtcggc
    14521 gctgcatccg cgttatagcc gcgcccagta tgaccgggta atggaagaac tttacccgcg
    14581 tctgaaatca ctcaccctgc tggcgcgtca gtatgatatt ggtattaaca tcgatgccga
    14641 agaggccgat cgtctggaga tctccctcga tctgctggaa aaactctgtt tcgagccgga
    14701 actggcaggc tggaacggca tcggttttgt tattcaggct tatcaaaaac gctgcccgtt
    14761 ggtgattgat tacctgattg atctcgccac ccgtagccgt cgccgtctga tgattcgtct
    14821 ggtgaaaggc gcgtactggg atagtgaaat taagcgtgcg cagatggacg gccttgaagg
    14881 ttatccggtt tatacccgca aggtgtatac cgacgtttct tatctcgcct gtgcgaaaaa
    14941 actgctggcg gtgccgaatc taatctaccc gcagttcgcg acgcacaacg cccatacgct
    15001 ggcagcgatt tatcaactgg caggtcagaa ctactatccg ggtcagtacg agttccagtg
    15061 cctgcatggt atgggcgagc cactgtacga gcaggtcacc gggaaagttg ccgacggtaa
    15121 acttaaccga ccgtgtcgta tttatgcccc ggttggcaca catgaaacgc tgctggcgta
    15181 tctggtgcgt cgcctgctgg aaaacggtgc taacacctcg tttgttaacc gtattgccga
    15241 cacctctttg ccactggatg aactggtcgc cgatccggtc actgctgtag aaaaactggc
    15301 gcaacaggaa gggcaaactg gattaccgca tccgaaaatt cccctgccgc gcgatcttta
    15361 cggtcacggg cgcgacaact cggcagggct ggatctcgct aacgaacacc gtctggcctc
    15421 gctctcctct gccctgctca atagtgcact gcaaaaatgg caggccttgc caatgctgga
    15481 acaaccggta gcggcaggtg agatgtcgcc cgttattaac cctgcggaac cgaaagatat
    15541 tgtgggctat gtgcgtgaag ccacgccgcg tgaggtagaa caggcgctgg aaagtgcggt
    15601 taataacgcg ccaatctggt ttgccactcc tccggttgaa cgcgcggcga ttttgcatcg
    15661 cgctgccgtg ctaatggaaa gccagatgca gcaactgatt ggtattctgg tgcgtgaagc
    15721 cggaaaaacc ttcagtaacg ccattgccga agtgcgcgaa gcggtcgatt ttctccacta
    15781 ctatgccgga caggtgcggg atgatttcgc taacgaaacc caccgtccat tagggcctgt
    15841 ggtgtgtatc agtccgtgga acttcccgct ggctattttc accgggcaga ttgctgccgc
    15901 actggcggca ggtaacagcg tgctggcaaa accggcagaa caaacgccgc tgattgccgc
    15961 gcaagggatc gccattttgc tggaagcggg tgtaccgcca ggcgtggtgc agttgctgcc
    16021 aggtcagggg gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtac gcggggtgat
    16081 gtttaccggt tcaaccgaag tcgctacgtt actgcagcgc aatatcgcca gccgcctgga
    16141 cgctcagggt cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg cgatgattgt
    16201 cgattcttca gcactgaccg aacaggtcgt cgtggatgta ctggcctcgg cgttcgacag
    16261 tgcgggtcag cgttgttcgg cgctgcgcgt gctgtgcctg caagatgaga ttgccgacca
    16321 cacgttgaaa atgctgcgcg gcgcaatggc cgaatgccgg atgggtaatc cgggtcgcct
    16381 gaccaccgat atcggtccgg tgattgatag cgaagcgaaa gccaatattg agcgccatat
    16441 tcagaccatg cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa acagcgaaga
    16501 tgcccgtgaa tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac tggatgactt
    16561 tgccgaattg caaaaagagg tctttggtcc ggtgctgcat gtggtgcgtt acaaccgtaa
    16621 ccagctacca gagctgatcg agcagattaa cgcttccggc tatggtctga cgcttggtgt
    16681 ccatacgcgt attgatgaaa ccatcgccca ggtcactggc tcggcccatg ttggtaacct
    16741 gtatgttaac cgtaatatgg tgggcgcagt ggtgggtgtg cagccattcg gcggcgaagg
    16801 gttgtccggt accgggccga aagcaggcgg tccgctctat ctctaccgtc tgctggcgaa
    16861 tcgcccggaa agtgcgctgg cagtgacgct cgcgcgtcag gatgcagagt atccggtcga
    16921 tgcacagttg aaagccgcat tgactcagcc gctaaatgca ctgcgggaat gggcagcaaa
    16981 tcgtccagaa ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg caggaacaca
    17041 acgattgctg ccggggccga cgggtgaacg caacacctgg acgctgctgc cgcgtgagcg
    17101 cgtgttgtgt attgccgatg atgagcagga tgcgctgact cagctcgccg ccgtgctggc
    17161 ggtgggcagc caggtactgt ggccggatga cgcgctgcat cgtcagctag taaaggcatt
    17221 gccatcggca gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa ccgctcaacc
    17281 ctttgatgcg gtgatcttcc acggtgattc ggatcagctt cgcgcattgt gtgaagccgt
    17341 tgctgcgcgg gatggcgcaa ttgtttcggt gcagggtttt gcccgtggcg aaagcaatat
    17401 ccttctggaa cggctgtata tcgaacgttc gctgagtgtg aataccgctg ccgctggcgg
    17461 taacgccagt ttaatgacta taggttaaac aggtagccgg aggatgttac aacctcctcc
    17521 ggcatcttta acgtggtcga atcccctcaa taataatccg ctgcacgttt tcaaccgttt
    17581 gattgaaaaa tatctcatcg cgcaacgtcg cgcctgtcac cgcctccacc tgaggggcga
    17641 aatcggcgta atgttgagtg gaagcccaaa tcatgaaaat caaatgctgc ggatcaatcg
    17701 gcgcgagttt gccgcttttg acccaaccgg caatcagcgc cgatttctca tcaattaatg
    17761 ccttcaaatc gcccgtcagt tcatccatta acagcggcgc gcctgccagc atctccatac
    17821 agaacaggcg cgaagcctgc ggataatcgc gggagacttc cagcttcaga cggatgtact
    17881 cttttatcgc cgccagcgga gcgaaatctt cacgaaacgc ttttaacggt gccagccaga
    17941 tatcgagaat ctgccgcagc acggcaatat acagcgcctc ttttgacgga aagtaataca
    18001 gcagattggt ttttgaaaca cccgccaact ctgcgatctg ctccagcctt gtgccgtgaa
    18061 aaccgaattg tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc ttcgcgctta
    18121 ctgcgcgcga acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc tctcccctct
    18181 tgtagattgg caacaggata gcaaaacccc cgcccggcct cgaccttctg cactctcatc
    18241 gcgcacagat gcatgtttta tgtgcaactg ttttgaccgt ttagtccact ttttaccaga
    18301 tattttagac aacccaatgt taacacatta ataacattca ctttttaaaa actggcatcc
    18361 gctttgcaaa caagccatta cgcggctgaa aaggatatgc aggatgcagc gccccgtctg
    18421 actttcactt tacgagacga agagaggttg atgatgaaaa ttggcgtatt cgtacctatt
    18481 ggcaacaacg gctggctcat ttcgacccac gcgccgcagt acatgccgac ctttgaactg
    18541 aataaagcca ttgtgcaaaa agcggagcac taccatttcg atttcgccct gtcgatgatc
    18601 aaactgcgtg gctttggcgg caaaactgag ttctgggatc acaaccttga gtcgttcacc
    18661 ttgatggcgg ggctggcagc cgtcacctca cgcattcaga tttacgcgac agctgccacc
    18721 ttaacgttac ctccggcaat cgtcgcccgt atggccgcaa ccattgactc catctctggc
    18781 gggcgttttg gcgtcaacct cgtgactggc tggcaaaagc ccgagtatga gcagatgggt
    18841 atctggcctg gcgatgacta tttctcccgt cgttacgact atctcaccga atatgttcag
    18901 gtgctgcgcg acctgtgggg ctcggggaaa agcgatttta aaggcgattt tttcaccatg
    18961 aatgattgtc gcgtcagtcc gcaaccgagt gtacccatga aagtgatctg cgccgggcaa
    19021 agcgacgctg gcatggcgtt ctccgcccag tatgccgatt tcaacttctg tttcggcaaa
    19081 ggcgtaaata cacccacggc tttcgccccg accgctgcgc ggatgaaaca ggccgcagag
    19141 caaaccgggc gcgacgttgg ctcttatgta ttgtttatgg tgattgccga tgaaaccgac
    19201 gatgccgctc gcgccaaatg ggaacactac aaagcgggcg cggatgaaga ggcgttaagc
    19261 tggctaaccg aacaaagtca gaaagatacc cgctccggta ctgacaccaa cgtccgtcag
    19321 atggccgatc ccacttcggc ggtaaacatc aatatgggga cgttagtcgg ttcttacgcc
    19381 agtgtcgcgc gcatgttaga tgaagtcgca agcgtgcctg gtgccgaagg cgtgctgttg
    19441 accttcgatg attttctgtc gggaatcgaa accttcggcg agcgcattca accactgatg
    19501 cagtgccgcg cccatctccc tgcgc
//
LOCUS       DAEVZA010000094        19322 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEVZA010000094 DAEVZA010000000
VERSION     DAEVZA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.094"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(19..1302)
                     /gene="gltA"
                     /locus_tag="LAF92_003397"
     CDS             complement(19..1302)
                     /gene="gltA"
                     /locus_tag="LAF92_003397"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBI9792351.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1508..1615)
                     /locus_tag="LAF92_003398"
     CDS             complement(1508..1615)
                     /locus_tag="LAF92_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792352.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            1996..2400
                     /gene="sdhC"
                     /locus_tag="LAF92_003399"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             1996..2400
                     /gene="sdhC"
                     /locus_tag="LAF92_003399"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBI9792353.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2394..2741
                     /gene="sdhD"
                     /locus_tag="LAF92_003400"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2394..2741
                     /gene="sdhD"
                     /locus_tag="LAF92_003400"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBI9792354.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2741..4507
                     /gene="sdhA"
                     /locus_tag="LAF92_003401"
     CDS             2741..4507
                     /gene="sdhA"
                     /locus_tag="LAF92_003401"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBI9792355.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4523..5239
                     /gene="sdhB"
                     /locus_tag="LAF92_003402"
     CDS             4523..5239
                     /gene="sdhB"
                     /locus_tag="LAF92_003402"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBI9792356.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5440..8241
                     /gene="sucA"
                     /locus_tag="LAF92_003403"
     CDS             5440..8241
                     /gene="sucA"
                     /locus_tag="LAF92_003403"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBI9792357.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8256..9473
                     /gene="odhB"
                     /locus_tag="LAF92_003404"
     CDS             8256..9473
                     /gene="odhB"
                     /locus_tag="LAF92_003404"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBI9792358.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9567..10733
                     /gene="sucC"
                     /locus_tag="LAF92_003405"
     CDS             9567..10733
                     /gene="sucC"
                     /locus_tag="LAF92_003405"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBI9792359.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10733..11602
                     /gene="sucD"
                     /locus_tag="LAF92_003406"
     CDS             10733..11602
                     /gene="sucD"
                     /locus_tag="LAF92_003406"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBI9792360.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11706..12428)
                     /gene="mngR"
                     /locus_tag="LAF92_003407"
     CDS             complement(11706..12428)
                     /gene="mngR"
                     /locus_tag="LAF92_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBI9792361.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12597..14513
                     /gene="mngA"
                     /locus_tag="LAF92_003408"
     CDS             12597..14513
                     /gene="mngA"
                     /locus_tag="LAF92_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBI9792362.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGDTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVRVAEPIRHAEALMQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFCLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14531..17164
                     /gene="mngB"
                     /locus_tag="LAF92_003409"
     CDS             14531..17164
                     /gene="mngB"
                     /locus_tag="LAF92_003409"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBI9792363.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEASKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            17189..17416
                     /locus_tag="LAF92_003410"
     CDS             17189..17416
                     /locus_tag="LAF92_003410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792364.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            18011..>19322
                     /gene="cydA"
                     /locus_tag="LAF92_003411"
     CDS             18011..>19322
                     /gene="cydA"
                     /locus_tag="LAF92_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBI9792365.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAV"
BASE COUNT         4658 a         4762 c         5208 g         4694 t
ORIGIN      
        1 caacttagca atcaaccatt aacgcttgat atcgctttta aagtcgcgtt tttcatatcc
       61 tgtatacagc tgacgcggac gggcaatctt cataccgtca ctgtgcattt cgctccagtg
      121 ggcgatccag ccaacggtac gtgccattgc gaaaatgacg gtgaacatgg aagacggaat
      181 acccatcgct ttcaggatga taccagagta gaaatcgacg ttcgggtaca gtttcttctc
      241 gataaagtac gggtcgttca gcgcgatgtt ttccagctcc atagccactt ccagcaggtc
      301 atctttcgtg cccaactctt tcagaacttc atggcaggtt tcacgcatta cggtggcgcg
      361 cgggtcgtaa tttttgtata cgcggtgacc gaagcccatc aggcggaaag aatcattttt
      421 atctttcgca cgacgaacaa attccggaat gtgtttaacg gagctaattt cttccagcat
      481 tttcagcgcc gcttcgttag caccaccgtg cgcaggtccc cacagtgaag caatacctgc
      541 tgcgatacag gcaaacgggt tcgcacccga agagccagcg gtacgcacgg tggaggtaga
      601 ggcgttctgt tcatggtcag cgtgcaggat cagaatacgg tccatagcac gttccagaat
      661 cggattaact tcatacggtt cgcacggcgt ggagaacatc atattcagga agttaccggc
      721 ataggagaga tcgttgcgcg gataaacaaa tggctgacca atggaatact tgtaacacat
      781 cgcggccatg gtcggcattt tcgacagcag gcggaacgcg gcaatttcac gatgacgagg
      841 attgttaaca tccagcgagt cgtgatagaa cgccgccagc gcgccggtaa taccacacat
      901 gactgccatt ggatgcgagt cgcgacggaa agcatggaac agacgggtaa tctgctcgtg
      961 gatcatggta tgacgggtca ccgtagtttt aaattcgtca tactgttcct gagtcggttt
     1021 ttcaccattc agcaggatgt aacaaacttc caggtagtta gaatcggtcg ccagctgatc
     1081 gatcgggaaa ccgcggtgca gcaaaatacc ttcatcacca tcaataaaag taattttaga
     1141 ttcgcaggat gcggttgaag tgaagcctgg gtcaaaggtg aacacacctt ttgaaccgag
     1201 agtacggata tcaataacat cttgacccag cgtgcctttc agcacatcca gttcaacagc
     1261 tgtgtccccg ttgagggtga gttttgcttt tgtatcagcc atttaaggtc tccttagcgc
     1321 cttattgcgt aagactgccg gaacttaaat ttgccttcgc acatcaacct ggctttaccc
     1381 gtttgttatt tggctcgccg ctctgtgaaa gaggggaaaa cctgggtaca gagctctggg
     1441 cgcttgcagg taaaggatcc attgatgacg aataaatggc gaatcaagta cttagcaatc
     1501 cgaattatta aacttgtcta ccactaataa ctgtcccgaa tgaattggtc aatacttcca
     1561 cactgttaca taagttaatc ttaggtgaaa taccgacttc ataactttta cgcattatat
     1621 gcttttcctg gtaatgtttg taacaacttt gttgaatgat tgtcaaatta gatgattaaa
     1681 aattaaataa atgttgttat cgtgacctgg atcactgttc aggataaaac ccgacaaact
     1741 atatgtaggt taattgtaat gattttgtga acagcctata ctgccgccag gtctccggaa
     1801 caccctgcaa tcccgagcca cccagcgttg taacgtgtcg ttttcgcatc tggaagcagt
     1861 gttttgcatg acgcgcagtt atagaaagga cgctgtctga cccgcaagca gaccggagga
     1921 aggaaatccc gacgtctcca ggtaacagaa agttaacctc tgtgcccgta gtccccaggg
     1981 aataataaga acagcatgtg ggcgttattc atgataagaa atgtgaaaaa acaaagacct
     2041 gttaatctgg acctacagac catccggttc cccatcacgg cgatagcgtc cattctccat
     2101 cgcgtttccg gtgtgatcac ctttgttgca gtgggcatcc tgctgtggct tctgggtacc
     2161 agcctctctt cccctgaagg tttcgagcaa gcttccgcga ttatgggcag cttcttcgtc
     2221 aaatttatca tgtggggcat ccttaccgct ctggcgtatc acgtcgtcgt aggtattcgc
     2281 cacatgatga tggattttgg ctatctggaa gaaacattcg aagcgggtaa acgctccgcc
     2341 aaaatctcct ttgttattac tgtcgtgctt tcacttctcg caggagtcct cgtatggtaa
     2401 gcaacgcctc cgcattagga cgcaatggcg tacatgattt catcctcgtt cgcgctaccg
     2461 ctatcgtcct gacgctctac atcatttata tggtcggttt tttcgccacc agtggcgaac
     2521 tgacatatga agtctggatc ggtttcttcg cctctgcgtt caccaaagtg ttcaccctgc
     2581 tggcgctgtt ttctatcttg attcatgcct ggatcggcat gtggcaggtg ttgaccgact
     2641 atgttaaacc gctggcattg cgcctgatgc tgcaactggt gattgtcgtt gcactggtgg
     2701 tttacgtgat ttatggattc gttgtggtgt ggggtgtgtg atgaaattgc cagtcagaga
     2761 atttgatgca gttgtgattg gtgccggtgg cgcaggtatg cgcgcggcgc tgcaaatttc
     2821 ccagagcggc cagacctgtg cgctgctctc taaagtcttc ccgacccgtt cccataccgt
     2881 ttctgcgcaa ggcggcatta ccgttgcgct gggtaatacc catgaagata actgggaatg
     2941 gcatatgtac gacaccgtaa aagggtcgga ctatatcggt gaccaggacg cgattgaata
     3001 tatgtgtaaa accgggccgg aagcgattct ggaactcgaa cacatgggcc tgccgttctc
     3061 gcgtcttgat gatggtcgta tctatcaacg tccgtttggc ggtcagtcga aaaacttcgg
     3121 tggcgagcag gcggcacgca ctgcggcggc ggctgaccgt accggtcacg cactgttgca
     3181 cacgctttat cagcagaacc tgaaaaacca caccaccatt ttctccgagt ggtatgcgct
     3241 ggatctggtg aagaaccagg atggcgcggt ggtgggttgt actgcactgt gcatcgaaac
     3301 tggtgaagtg gtttatttca aagcccgcgc taccgtgctg gcgactggcg gggcagggcg
     3361 tatttatcag tccaccacta acgcccacat taacactggc gacggcgtcg gcatggctat
     3421 ccgcgcaggc gtaccggtgc aggatatgga aatgtggcag ttccacccga ccggtattgc
     3481 cggtgcgggc gtactggtca ccgaaggttg ccgtggtgaa ggcggttatc tgctgaacaa
     3541 acatggcgaa cgctttatgg agcgttatgc gccgaacgcc aaagacctgg cgggccgtga
     3601 tgtggtggcg cgttccatca tgatcgaaat ccgtgaaggt cgcggctgtg atggtccgtg
     3661 ggggccacac gcgaaactga aactcgatca cctgggtaaa gaagttctcg aatcccgtct
     3721 gccgggtatc ctggagcttt cccgtacctt cgctcacgtc gatccggtga aagagccgat
     3781 tccggttatc ccaacctgtc actacatgat gggcggtatt ccgaccaaag ttaccggtca
     3841 ggcgctgact gtgaatgaga aaggcgaaga tgtggttgtt ccgggactgt ttgccgttgg
     3901 tgaaatcgct tgtgtatcgg tacacggcgc taaccgtctg ggcggcaact cgctgctgga
     3961 cctggtggtc tttggtcgcg cggcaggtct gcatctgcaa gagtctatcg ccgagcaggg
     4021 cgcactgcgc gatgccagcg agtctgatgt tgaagcgtct ctggatcgcc tgaaccgctg
     4081 gaacaataat cgtaacggtg aagatccggt ggcgattcgt aaagcgctgc aagaatgtat
     4141 gcagcataac ttctcggtct tccgtgaagg tgatgcgatg gcgaaagggc ttgagcagtt
     4201 gaaagtgatc cgcgagcgtc tgaaaaatgc ccgtctggat gacacttcca gcgagttcaa
     4261 cacccagcgc gttgagtgcc tggaactgga taacctgatg gaaacggcgt atgcaacggc
     4321 tgtttctgcc aacttccgta ccgaaagccg tggcgcacat agccgcttcg acttcccgga
     4381 tcgtgatgat gaaaactggc tgtgccactc cctgtatctg ccagagtcgg aatccatgac
     4441 gcgccgaagc gtcaacatgg aaccgaaact gcgcccggca ttcccgccga agattcgtac
     4501 ttactaatgc ggagacagga aaatgagact cgagttttca atttatcgct ataacccgga
     4561 tgttgatgat gctccgcgta tgcaggatta caccctggaa gcggaagaag gtcgcgacat
     4621 gatgttgctg gatgcgctta tccagctgaa agagaaagat cccagcctgt cgttccgccg
     4681 ctcctgccgt gaaggtgtgt gcggttccga cggtctgaac atgaacggca agaatggtct
     4741 ggcctgtatt accccgattt cggcactcaa ccagccgggc aagaagattg tgattcgccc
     4801 gctgccaggt ttaccggtga tccgcgattt ggtggtagac atgggacaat tctatgcgca
     4861 atatgagaaa attaagcctt acctgttgaa taatggacaa aatccgccag ctcgcgagca
     4921 tttacagatg ccagagcagc gcgaaaaact cgacgggctg tatgaatgta ttctctgcgc
     4981 atgttgttca acctcttgtc cgtctttctg gtggaatccc gataagttta tcggcccggc
     5041 aggcttgtta gcggcatatc gtttcctgat cgatagccgt gataccgaga ctgacagccg
     5101 cctcgacggt ttgagcgatg cattcagtgt attccgctgt cacagcatca tgaactgcgt
     5161 cagtgtatgt ccgaaggggc tgaacccgac gcgcgccatc ggccatatca agtcgatgtt
     5221 gttgcaacgt aatgcgtaat taaatgttga tgccggggaa atcccccggc tattcaggaa
     5281 atctttaaaa actgcccctg acactaagac agtttttaaa ggttccttcg cgagccacta
     5341 cgtagacaag agctcgcaag tgaaccccgg cacgcacata actgtgcgtg gtagtatcca
     5401 cggcgaagta agcataaaaa agatgcttaa gggatcacga tgcagaacag cgctttgaaa
     5461 gcctggttgg actcttctta cctctctggc gcaaaccaga gctggataga acagctctat
     5521 gaagacttct taaccgatcc tgactcggtt gacgctaact ggcgttcgac gttccagcag
     5581 ttacctggta cgggagtcaa accggatcaa ttccactctc aaacgcgtga atatttccgc
     5641 cgcctggcga aagacgcttc acgttactct tcaacgatct ccgaccctga caccaatgta
     5701 aagcaggtta aagtcctgca gctcattaac gcataccgct tccgtggtca ccagcatgcg
     5761 aatctcgatc cgctgggact gtggcagcaa gataaagtgg ccgatctgga tccgtctttc
     5821 cacgatctga ccgaagcaga cttccaggag accttcaacg tcggttcatt tgccagcggc
     5881 aaagaaacca tgaaactcgg cgagctgctg gaagccctca agcaaaccta ctgcggcccg
     5941 attggtgccg agtatatgca cattaccagc accgaagaaa aacgctggat ccaacagcgt
     6001 atcgagtctg gtcgcgcgac tttcaatagc gaagagaaaa aacgcttctt aagcgaactg
     6061 accgccgctg aaggccttga acgttacctc ggcgcaaaat tccctggcgc aaaacgcttc
     6121 tcgctggaag gcggtgacgc gttaatcccg atgcttaaag agatgatccg ccacgctggc
     6181 aacagcggca cccgggaagt ggttctcggg atggcgcacc gtggtcgtct gaacgtgttg
     6241 gtgaacgtgc tgggtaaaaa accgcaagac ttgttcgacg agtttgccgg taaacataaa
     6301 gaacacctcg gcacgggtga cgtgaaatac cacatgggct tctcgtctga cttccagacc
     6361 gatggcggcc tggtgcacct ggcgctggcg tttaacccgt ctcaccttga gattgtaagc
     6421 ccggtcgtta tcggctctgt tcgtgcccgt ctggacagac ttgatgagcc gagcagcaac
     6481 aaagtgctgc caattaccat ccacggtgac gccgcagtga ccggacaggg cgtggttcag
     6541 gaaaccctga acatgtcgaa agcgcgtggt tatgaagttg gcggtacggt acgtatcgtt
     6601 atcaacaacc aggttggttt caccacctct aatccgctgg atgcccgttc tacgccgtac
     6661 tgtactgata tcggcaagat ggttcaggca ccgattttcc acgttaacgc ggacgatccg
     6721 gaagccgttg cctttgtgac ccgtctggcg ctcgatttcc gtaacacctt taaacgtgat
     6781 gtcttcatcg acctggtatg ctaccgccgt cacggccaca acgaagccga cgagccgagc
     6841 gcaacccagc cgctgatgta tcagaaaatc aaaaaacatc cgacaccgcg caaaatctac
     6901 gctgacaagc tggagcagga aaaagtggcg acgctggaag atgccaccga gatggttaac
     6961 ctgtaccgcg atgcgctgga tgctggcgat tgcgtggtag cagagtggcg tccgatgaac
     7021 atgcactctt tcacctggtc gccgtacctc aaccacgaat gggacgaaga gtacccgaac
     7081 aaagttgaga tgaagcgcct gcaggaactg gctaaacgca tcagcacggt gccggaagcg
     7141 gttgaaatgc agtctcgcgt tgccaagatt tatggcgatc gccaggcgat ggctgccggt
     7201 gagaaactgt tcgactgggg cggcgcggaa aacctcgctt acgccacgct ggttgacgaa
     7261 ggcattccgg ttcgcctgtc gggtgaagac tccggtcgcg gtaccttctt ccaccgccac
     7321 gcggtgatcc acaaccagtc taacggttcc acttacacgc cgttgcaaca catccataac
     7381 gggcagggcg cgttccgcgt ctgggactcc gtactgtctg aagaagcagt gctggcgttt
     7441 gaatacggtt atgccaccgc agaaccacgc actctgacca tctgggaagc gcagttcggt
     7501 gacttcgcca acggtgcgca ggtggttatc gaccagttca tctcctctgg cgagcagaaa
     7561 tggggccgga tgtgtggtct ggtgatgttg ctgccgcacg gttacgaagg gcaggggccg
     7621 gagcactcct ccgcgcgtct ggaacgttat ctgcaacttt gtgctgagca aaacatgcag
     7681 gtttgcgtac cgtctacccc ggcacaggtt taccacatgc tgcgtcgtca ggcgctgcgc
     7741 gggatgcgtc gtccgctggt cgtgatgtcg ccgaaatccc tgctgcgtca tccgctggcg
     7801 gtttccagcc tcgaagaact ggcgaacggc accttcctgc cagccatcgg tgaaatcgac
     7861 gagcttgatc cgaagggcgt gaagcgcgta gtaatgtgtt ctggtaaggt ttattacgac
     7921 ctgctggaac agcgtcgtaa gaacaatcaa cacgatgtcg ccattgtgcg tatcgagcaa
     7981 ctctacccgt tcccgcataa agcgatgcag gaagtgttgc agcagtttgc tcacgtcaag
     8041 gattttgtct ggtgccagga agagccgctc aaccagggcg catggtactg cagccagcat
     8101 catttccgtg aagtgattcc gtttggggct tctctgcgtt atgcaggccg cccggcctcc
     8161 gcctctccgg cggtagggta tatgtccgtt caccagaaac agcaacaaga tctggttaat
     8221 gacgcgctga acgtcgaata aataaaggat acacaatgag tagcgtagat attctggtcc
     8281 ctgacctgcc tgaatccgta gccgatgcca ccgtcgcaac ctggcataaa aaacccggcg
     8341 acgcagtcgt acgtgatgaa gtgctggtag aaatcgaaac tgacaaagtg gtactggaag
     8401 taccggcatc agcagacggc attctggatg cggttctgga agatgaaggt acaacggtaa
     8461 cgtctcgtca gatccttggt cgcctgcgtg aaggcaacag cgccggtaaa gaaaccagcg
     8521 ccaaatctga agagaaagcg tccactccgg cgcaacgcca gcaggcgtct ctggaagagc
     8581 aaaacaacga tgcgttaagc ccggcgatcc gtcgcctgct ggctgaacac aatctcgacg
     8641 ccagcgccat taaaggcacc ggtgtgggtg gtcgtctgac tcgtgaagat gtggaaaaac
     8701 atctggcgaa agccccggcg aaagagtctg ctccggcagc ggctgatccg gcggcgcaac
     8761 cggctctggc tgcacgtagt gaaaaacgtg tcccgatgac tcgcctgcgt aagcgtgtgg
     8821 cagagcgtct gctggaagcg aaaaactcca ccgccatgct gaccacgttc aacgaagtca
     8881 acatgaagcc gattatggat ctgcgtaagc agtacggtga agcgtttgaa aaacgccacg
     8941 gcatccgtct gggctttatg tccttctacg tgaaagcggt ggttgaagcc ctgaaacgtt
     9001 acccggaagt gaacgcgtct atcgacggcg atgacgtggt ttaccacaac tatttcgacg
     9061 tcagcatggc ggtttctacg ccgcgcggcc tggtgacacc ggtactgcgt gatgtcgata
     9121 ccctcggcat ggcagacatc gagaagaaaa tcaaagagct ggcagtcaaa ggccgtgacg
     9181 gcaagctgac cgttgaagat ctgaccggtg gtaacttcac catcaccaac ggtggtgtgt
     9241 tcggttccct gatgtctacg ccgatcatca acccgccgca gagcgcaatt ctgggtatgc
     9301 acgctatcaa agatcgtccg atggcggtga atggtcaggt tgagatcctg ccgatgatgt
     9361 acctggcgct gtcctacgat caccgtctga tcgatggtcg cgaatccgtg ggcttcctgg
     9421 taacgatcaa agagttgctg gaagatccga cgcgtctgct gctggacgtg tagtagttta
     9481 agcttcacct gcactgtaga ccgacgtgga tagggtgtcg gtctacggtt taaaagataa
     9541 cgattactga aggatggaca gaacacatga acttacatga atatcaggca aaacaacttt
     9601 ttgcccgcta tggcttacca gcaccggtgg gttatgcctg tactactccg cgcgaagcag
     9661 aagaagccgc ttcaaaaatc ggtgccggcc cgtgggtagt gaaatgtcag gttcacgctg
     9721 gtggccgcgg taaagcgggc ggtgtgaaag ttgtaaacag caaagaagac atccgtgctt
     9781 ttgcagaaaa ctggctcggc aagcgtctgg taacgtatca aacagatgcc aatggccaac
     9841 cggttaacca gattctggtt gaagcagcga ccgatatcgc taaagagctg tatctcggtg
     9901 ccgttgtaga ccgtagttcc cgtcgcgtgg tctttatggc ctccaccgaa ggcggcgtgg
     9961 aaatcgaaaa agtggcggaa gaaactccgc acctgatcca taaagttgcg cttgatccgc
    10021 tgacaggccc gatgccgtat cagggacgcg agctggcgtt caaactgggt ctggaaggta
    10081 aactggttca gcagttcacc aaaatcttca tgggcctggc gaccattttc ctggagcgcg
    10141 acctggcgtt gatcgaaatc aacccgctgg tcatcaccaa acagggcgat ctgatttgcc
    10201 tcgacggcaa actgggcgct gacggcaacg cactgttccg ccagcctgat ctgcgcgaaa
    10261 tgcgtgacca gtcgcaggaa gatccgcgtg aagcacaggc tgcacagtgg gaactgaact
    10321 acgttgcgct ggacggtaac atcggttgta tggttaacgg cgcaggtctg gcgatgggta
    10381 cgatggacat cgttaaactg cacggcggcg aaccggctaa cttccttgac gttggcggcg
    10441 gcgcaaccaa agaacgtgta accgaagcgt tcaaaatcat cctctctgac gacaaagtga
    10501 aagccgttct ggttaacatc ttcggcggta tcgttcgttg cgacctgatc gctgacggta
    10561 tcatcggcgc ggtagcagaa gtgggtgtta acgtaccggt cgtggtacgt ctggaaggta
    10621 acaacgccga actcggcgcg aagaaactgg ctgacagcgg cctgaatatt attgcagcaa
    10681 aaggtctgac ggatgcagct cagcaggttg ttgccgcagt ggaggggaaa taatgtccat
    10741 tttaattgat aaaaacacca aggttatctg ccagggcttt actggtagcc aggggacttt
    10801 ccactcagaa caggccattg catacggcac taaaatggtt ggcggcgtaa ccccaggtaa
    10861 aggcggtact acccacctcg gcctgccggt gttcaacacc gtgcgtgaag ctgttgctgc
    10921 tactggcgct accgcttctg ttatctacgt accagcaccg ttctgcaaag actccattct
    10981 ggaagctatt gatgcaggca tcaaactgat tatcaccatc actgaaggca tcccgacgct
    11041 ggatatgctg actgtgaaag tgaaactgga cgaagccggc gttcgtatga tcggcccgaa
    11101 ctgcccgggc gttatcactc cgggtgaatg caaaatcggt atccagccgg gtcacattca
    11161 caaaccgggt aaagtgggta tcgtttcccg ttccggtaca ctgacctatg aagcggttaa
    11221 acagaccacg gattacggtt tcggtcagtc gacctgtgtt ggtatcggcg gtgacccgat
    11281 ccctggctct aactttatcg acattctcga aatgttcgaa aaagatccgc agaccgaagc
    11341 gatcgtaatg atcggtgaga tcggcggtag cgctgaagaa gaagcggcag cgtacatcaa
    11401 agagcatgtt accaagccag ttgtgggtta catcgctggt gtgactgcgc cgaaaggcaa
    11461 acgtatgggc cacgcgggtg ccatcattgc cggtgggaaa gggactgcgg atgagaaatt
    11521 cgctgctctg gaagccgcag gcgtgaaaac cgttcgcagc ctggcggata tcggtgaagc
    11581 actgaaaact gttctgaaat aaatatctgt aataagaaat agccctcgcc gcttccctct
    11641 acaggaatgg cgaagggctg tcggtttcga catggttggc catcgtatga tggccttttt
    11701 tgtgcttatc gcgatgattt tcgttgcgct atcagggtaa atttatagtc atcggtatta
    11761 aaagcgttgc ggctatattc aaacacccga ccatcaacta aatatccacg cgatactttt
    11821 tcaagaatcg gctttgtctg gctgatatta agcagacggc tcatctcttc ggttggcatc
    11881 agaggaatga tttcctgttc gctacgatcg ataaccattt tcttcacttc ttcaataaag
    11941 tgatattttg aattttccat gacctgccag gtgagatccg ggaacaacgc aagcggcatc
    12001 caggtttctt ccagcgccat tggcttttgc ttgcgatagc gcacgcgctt cacatgccac
    12061 acacgatcct gcgcggtgat ttgtagctgt tgctgaagaa aatcgtcagc cggaatcact
    12121 tcaaatatca ggacttcact gtgtgtatcg acgtgacggt ccgacagttt ttcatcaaaa
    12181 ctggttaact gaaaaatatc gtaattgacc cgctcttctt tgacgtaagt cccgctgccc
    12241 tgaatgcttt cgaggatctg ttgctcgact aactggcgca aagcctgacg caccgtaacc
    12301 cggctgacgc caaactctgt ttgtaatgcc gattcggtag gtaacgcatc gccaggttta
    12361 agctcgccac gcgcaatttg ttcacgaatg cgatcggcaa tctgccggta taagggcttg
    12421 tgtcccattt ttagtatctc attaatacga atttagccat tatgcccgat aaattcatcc
    12481 tgtaaataat acaaatacaa tacaaataat ttcaatcaag tgaaattgat cacataatgg
    12541 tattgtttta tcgggcacac tggcgcgact ataaaaacga tcaagtgagg atcatgatga
    12601 atctgacgac tctgacccac cgcgatgcgt tgtgtctgaa tgcgcgcttt accagccgtg
    12661 aagaggccat ccacgcgttg actcaacgtc ttgctgctct ggggaaaatt tccagtactg
    12721 agcaatttct ggaagaagtg tatcgccgtg aaagccttgg cccgaccgcc ttaggtgaag
    12781 ggttggctgt gccgcatggc aaaactgctg cggtaaaaga agcggcgttt gcggtcgcca
    12841 cactcagcga gccgcttcag tgggaaggcg ttgatggccc ggaagcggtt gatttagtgg
    12901 tgctgctggc gattcccccc aatgaagcgg gtactacgca tatgcaactg ctgacagcgc
    12961 tgaccacgcg cctggcggat gatgagattc gggcgcgtat acagtcggcg acgacgcctg
    13021 atgagttgct ctcggcgctg gatgacaagg gagacacgca accttctgcc tctttttcca
    13081 acgcgccaac tatcgtctgc gtaacggcct gtccggcggg tattgctcac acctatatgg
    13141 ctgcggaata tctggaaaaa gccggacgca aactcggcgt aaatgtttac gttgaaaaac
    13201 aaggcgctaa cggcattgaa gggcgtttaa cggcggatca actcaatagt gcaaccgcct
    13261 gtatttttgc ggctgaagtc gccatcaagg agagtgagcg ttttaatggc attcccgcgc
    13321 tttcagtgcg tgttgccgag ccgattcgcc atgcagaagc gttgatgcaa caagcgctta
    13381 ccctcaagcg tagcgatgag acgcgtaccg tacagcaaga tacgcaaccg gtgaaaagtg
    13441 tcaaaacgga gctgaaacag gcactgttga gcggaatctc ttttgccgta ccgttgattg
    13501 tcgcgggggg cacggtgctg gcggtcgcgg tattactgtc gcaaatcttc gggctacaag
    13561 atctgtttaa tgaagaaaac tcctggctgt ggatgtaccg caagctgggc ggcgggctgc
    13621 tcggaatttt gatggtaccg gtgctcgcgg cctataccgc ctattctctg gcagataaac
    13681 cggcgttagc gccaggcttt gcggctggac ttgccgccaa catgatcggc tccgggtttc
    13741 tcggcgcggt cgttggcgga ttgatagccg gttacttgat gcgctgggtg aaaaatcact
    13801 tgcgtcttag cagtaaattc aatggattcc tgactttttg tctctacccg gtgctcggta
    13861 cgttgggagc gggcagtctg atgctgtttg tggtgggaga acctgtcgcc tggatcaata
    13921 actcgcttac cgcctggctg aacggtctgt caggaagtaa cgcgctgttg ctgggtgcca
    13981 ttctcggttt tatgtgttcc tttgaccttg gagggccagt gaacaaagcc gcttatgcat
    14041 tctgcctggg cgcaatggcg aacggcgttt acggcccgta tgccattttc gcctccgtca
    14101 aaatggtttc ggcatttacc gtaaccgctt ccacgatgct cgcaccgcgc ctgtttaaag
    14161 agtttgaaat tgagaccggg aaatccacct ggctgttagg gctggcaggt attaccgaag
    14221 gggcgatccc gatggcgatt gaagatccgc tgcgggttat tggttcgttt gtgctgggct
    14281 ctatggtaac gggcgctatt gtcggtgcga tgaatatcgg cctttcgaca cccggtgccg
    14341 gcattttctc gctcttttta cttcatgata atggcgcggg cggtgttatg gcggcaattg
    14401 gctggtttgg tgcggcattg gtgggggctg caatctcgac tgcaattctc ctgatctggc
    14461 ggcgtcacgc ggttaagcat ggcaactatc tgactgatgg cgtaatgcca taaacaaaaa
    14521 ggaaacgacg atgaaagcgg tatctcgcgt tcacatcacc ccgcatatgc attgggatcg
    14581 ggagtggtat ttcaccaccg aagagtcacg cattctgctg gtcaataata tggaagagat
    14641 cctgtgccga ctggaacagg acaacgaata caaatattac gtactcgacg ggcaaacggc
    14701 gatcctcgaa gattatttcg cggtgaaacc ggaaaacaaa gaccgcgtga agaaacaggt
    14761 agaagccagt aagttgatta tcggcccctg gtatacccag accgatacca cgattgtttc
    14821 tgcggaatcc atcgtccgta atctgatgta cggaatgcgt gactgcctcg cgtttggcga
    14881 gccgatgaaa ataggttatt taccagattc ctttggcatg tccgggcaac tgccgcatat
    14941 ctacaatgga tttggcatta cccgcaccat gttctggcgc ggatgttcgg agcgccacgg
    15001 tactgataaa accgagtttt tgtggcaaag cagtgacggt agcgaagtga cggcgcaggt
    15061 gctgccgctg ggctacgcca tcggtaagta cttacctgcc gacgaaaacg gattacgtaa
    15121 acgtctcgac agttattttg acgtgctgga aaaagcgtct gtaaccaaag agattttgct
    15181 gccgaatggg catgaccaga tgccattgca gcaaaatatc ttcgaagtga tggataagct
    15241 acgtgagatc taccctcaac gtaagtttgt gatgagccgc tttgaagagg tatttgagaa
    15301 gatcgaagcg cagcgtgaaa gtctggccac cctgaaaggt gaatttattg atggcaaata
    15361 tatgcgcgtg catcgcacca tcggttctac gcgtatggat atcaaaattg cccacgcgcg
    15421 tattgaaaat aagattgtta atctgctgga accgctggca acgctggcct ggacgttggg
    15481 ttttgaatac caccacggct tgctggagaa aatgtggaaa gagatcttaa aaaatcatgc
    15541 ccacgacagt atcggttgtt gttgcagtga caaagttcat cgcgaaattg tcgcccgctt
    15601 cgaactggct gaagacatgg cggataatct gctgcgtttc tacatgcgca aaattgccga
    15661 taacatgcca cagagtgatg ccgacaaact cgtgctgttt aacctgatgc cctggccgcg
    15721 ggaagaagtt attaacacca ctgtgcggct gcgcgccagc cagtttaatc tacgagacga
    15781 tcgcggtcag cctgtaccgt attttattcg ccatgcccgt gagatcgatc caggcctaat
    15841 cgatcggcaa atagttcatt acggtaatta cgatcccttt atggagtttg atatacagat
    15901 caaccagatt gtcccttcta tgggctatcg cacgctttat atcgaagcga atcagcctgg
    15961 caacgtgatt gcggcaaaaa gtgacgctga agggatactg gaaaatgctt tctggcaaat
    16021 tgcgctcaat gaggatggaa cgctacaact ggtagataaa gatagcggtg tgcgctatga
    16081 ccgggtgttg caaattgaag aaagctctga tgatggtgat gaatatgact attcacccgc
    16141 aaaagaagag tgggtaatga cttctgcaac agcgaaaccg caatgcgaga ttactcatga
    16201 ggcctggcag agcagggctg ttatccgcta tgacatggcg gtgccgctca atttgttgga
    16261 acgaagcgtc cggcaatcca cgggcagagt aggggtggag atggttgtca ctctgagtca
    16321 taacagcaga cgtattgatg tggatatcaa ccttgataac caggctgacg atcatcgcct
    16381 tcgcgtcctg atccctacat cttttaacac cgatagtgtt ctggcagata cccagtttgg
    16441 ttcgctaacg cgccccgtga acgacagtgc aatgaacaac tggcagcaag aaggctggaa
    16501 agaagcgccg gttccggtat ggaatatgct caactatgca gccttacagg aagggcgtaa
    16561 cggcatggct gtctttagcg aagggctacg tgaatttgaa gtcatcggtg aagagaagaa
    16621 aacctttgcc attacgttgc tgcgtggcgt gggcttactg ggcaaagaag atctgttttt
    16681 aaggcctggg cggccttcgg gaattaaaat gccagtcccg gactcacaac tacgtggtct
    16741 gctttcttgt cgcctaagtt tattgagtta taccggtacg ccaaccgccg ctggtgtagc
    16801 tcagcaggcg cgagcatggc tgactccagt acagtgttac aacaaaatcc catgggatgc
    16861 gatgaagctc aacaaagccg gattcaacgt gccggaaagt tatagtttgt tgaaaatgcc
    16921 cccagtggga tgcctgataa gcgcacttaa gaaagctgaa gaccgacaag aagtgatttt
    16981 acggctgttt aatccggctg aatcagcaac ctgtgatgcg actgttgctt tcagtcgcga
    17041 ggtgatttct tgctcagaaa cgatgatgga tgaacacatt accaccgagg aaaatcaagg
    17101 ttcaaatcta tcggggcctt ttttacccgg ccagtcacgg acgttcagtt accggcttgc
    17161 ctgaatagca atcaaaccga agccacatat gcgcggccag attgttgaca aagggcgctt
    17221 tgttcatgcc ggatacggca tgaacgcttt attcggtcta caaaagcagg caaattcaat
    17281 atattgcaga gatgatgtag acactggcaa gcgtagcgca tcaggtaatt ttgcgtttat
    17341 cttcactctc aagccacgta tatgtggctt tatttttaac aaaataataa ccctgggtga
    17401 gttaattata atataattat aagttaatta aatgttaata tcagcggggt ggatttatgc
    17461 ctttattagt aatcctgaaa ctctgcgtcg tattagccag tgaccaaaaa aagaattaag
    17521 gtcaaccgtg ctgtttttgc ttcgtctctt tttatcttta attgccaacc gaaactaatt
    17581 tcagccttat aactcacaca ttttaaacat aaatgtcact aaagttacct tattgaaaca
    17641 tgattaacat aatttgtagg aattgatatt tatcaatgta taagtcttgg aaatgggcat
    17701 caaaaagaga taaattgttc tcgatcaaat tggctgaaag gcggtaattt agctataaat
    17761 tgatcaccgt cgaaaaatgc aaatttgctt caacaaaaac ctgtttattg taaggatttt
    17821 gcggcgtaat atatacgtgg gatcaatttg agtttttatt aacatgtttg caacctttct
    17881 ttacgccgtt tttgtgtgca ttcacatggt atgatgaaag tgttcaaaca aatttctatt
    17941 ggggcatgcg tgtgaccctt tctaacgggg ttcactctcg gagtcttcat gcgatgagca
    18001 aggagtcatg atgttagata tagtcgaact gtcgcgctta cagtttgcct tgaccgcgat
    18061 gtaccacttc ctttttgtgc cactgacgct cggtatggcg ttcctgctgg ccattatgga
    18121 aacggtctac gtcctctccg gcaaacagat ttataaagat atgaccaagt tctggggcaa
    18181 gttgtttggt atcaacttcg ctctgggtgt ggctaccggt ctgaccatgg agttccagtt
    18241 cgggactaac tggtcttact attcccacta tgtaggggat atcttcggtg cgccgctggc
    18301 aatcgaaggt ctgatggcct tcttcctcga atccaccttt gtaggtctgt tcttcttcgg
    18361 ttgggatcgt ctgggtaaag ttcagcatat gtgtgtcacc tggctggtgg cgctcggttc
    18421 aaacctgtcc gcactgtgga ttctggttgc gaacggctgg atgcaaaacc caatcgcgtc
    18481 cgatttcaac tttgaaacta tgcgtatgga gatggtgagc ttctccgagc tggtgcttaa
    18541 cccggttgct caggtgaaat tcgttcacac tgtagcgtct ggttatgtga ctggcgcgat
    18601 gttcatcctc ggtatcagcg catggtatat gctgaaaggt cgtgacttcg ccttcgctaa
    18661 acgctccttt gctatcgctg ccagcttcgg tatggctgct gttctgtctg ttattgttct
    18721 gggtgatgaa tccggctacg aaatgggcga cgtgcagaaa accaaactgg ctgctattga
    18781 agccgagtgg gaaacgcaac ctgcgcctgc tgcctttact ctgttcggca ttcctgatca
    18841 ggaagaggag acgaacaaat ttgcgattca gatcccttac gcactgggca tcattgcaac
    18901 gcgttccgtg gataccccgg ttatcggcct gaaagagctg atggtgcagc atgaagaacg
    18961 cattcgtaac gggatgaagg cgtactctct gctcgaacaa ctgcgttctg gttctaccga
    19021 ccaggcggtt cgtgaccagt tcaatagcat gaagaaagac ctcggttacg gtctgctgct
    19081 gaaacgctat acgccaaacg tggctgatgc gactgaagcg cagattcaac aggcaaccaa
    19141 agactccatc ccgcgtgtag cgccgctgta ctttgcgttc cgtatcatgg tggcgtgtgg
    19201 cttcctgctt ctggcaatca tcgcgctctc tttctggagt gtcatccgca accgcattgg
    19261 cgagaaaaaa tggcttctgc gcgccgcgct gtacggtatt ccgctgccgt ggattgctgt
    19321 ag
//
LOCUS       DAEVZA010000095        19217 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEVZA010000095 DAEVZA010000000
VERSION     DAEVZA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.095"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..528
                     /locus_tag="LAF92_003412"
     CDS             <1..528
                     /locus_tag="LAF92_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HBI9792366.1"
                     /translation="QSSCPCKARFIASMMDDTYETDDGGSEAEQHAQSAKKDLTSGSD
                     SSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDFTITNKGDEAEGLS
                     LEVLDGNEVIYSEQQTGKYFDKGEHAWQWDGYSNQGILDTTKLKSKSLLVRLIALCGD
                     MMIKVDYPLHNSPEE"
     gene            652..1206
                     /locus_tag="LAF92_003413"
     CDS             652..1206
                     /locus_tag="LAF92_003413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792367.1"
                     /translation="MVNLTKNGVEFYWSRNGSRGGGIGENIVTAIGVFKVNVKAEINI
                     TPSMRTFSLISSLVPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDS
                     AHEMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDM
                     YTRLVAADEDVQGLIWLSRIKFDD"
     gene            1199..1622
                     /locus_tag="LAF92_003414"
                     /pseudo
     CDS             1199..1622
                     /locus_tag="LAF92_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2182..3942
                     /gene="tssF"
                     /locus_tag="LAF92_003415"
     CDS             2182..3942
                     /gene="tssF"
                     /locus_tag="LAF92_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBI9792368.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSDFQVLSRPVGERRTRCFYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLNAGSPVACALHRALTLGTQQFWLRLPGQDRRTLDAHFS
                     PLGFEDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLELVAWPEGITGFEIDV
                     VLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDADRMLEDETLSLSLTGTNGHLPRKALQSTLLDRPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
     gene            3906..4985
                     /gene="tssG"
                     /locus_tag="LAF92_003416"
     CDS             3906..4985
                     /gene="tssG"
                     /locus_tag="LAF92_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBI9792369.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DNPATDAVRFRPHPGMGFPVSELKNVERDAGNPDVPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAMDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPALSIRNRESMTHVSYSF"
     gene            4966..5502
                     /gene="tssJ"
                     /locus_tag="LAF92_003417"
     CDS             4966..5502
                     /gene="tssJ"
                     /locus_tag="LAF92_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBI9792370.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            5506..5934
                     /gene="tssE"
                     /locus_tag="LAF92_003418"
     CDS             5506..5934
                     /gene="tssE"
                     /locus_tag="LAF92_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBI9792371.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWLRFDGVFSPEGRIVLRHLKQQERAY"
     gene            5934..7310
                     /locus_tag="LAF92_003419"
     CDS             5934..7310
                     /locus_tag="LAF92_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HBI9792372.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLSLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRALDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNAALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRPAQQNDK
                     DASPPVPSPEISRQRAWPIFMAGMVVMACLGGTGLWGWLQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSTEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEISPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            7375..7923
                     /locus_tag="LAF92_003420"
     CDS             7375..7923
                     /locus_tag="LAF92_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HBI9792373.1"
                     /translation="MYKKERNRPFETREEVLAYHKREKIPCLECGKLLIFLPRHIWFV
                     HGIRADEYREKWNIPKHIALAGISYLEKQSEYMKARIAKGKLDPAEQIAMMRIKRRQL
                     KSDPDWRNRSGSILKKQYARRDILKNRIWERSPSIKSVSVELKTEAIRRMKNRKVAGE
                     KVQDIADDLNVSISRLYHWLKM"
     gene            complement(8270..9523)
                     /gene="amiC"
                     /locus_tag="LAF92_003421"
     CDS             complement(8270..9523)
                     /gene="amiC"
                     /locus_tag="LAF92_003421"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBI9792374.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            9755..11086
                     /gene="argA"
                     /locus_tag="LAF92_003422"
     CDS             9755..11086
                     /gene="argA"
                     /locus_tag="LAF92_003422"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBI9792375.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(11148..12974)
                     /gene="recD"
                     /locus_tag="LAF92_003423"
     CDS             complement(11148..12974)
                     /gene="recD"
                     /locus_tag="LAF92_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBI9792376.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(12974..16516)
                     /gene="recB"
                     /locus_tag="LAF92_003424"
     CDS             complement(12974..16516)
                     /gene="recB"
                     /locus_tag="LAF92_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBI9792377.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQIGDNQPWQVNDVSTAELNAKTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYEHHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(16509..>19217)
                     /gene="ptrA"
                     /locus_tag="LAF92_003425"
     CDS             complement(16509..>19217)
                     /gene="ptrA"
                     /locus_tag="LAF92_003425"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBI9792378.1"
                     /translation="PQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYPQA
                     DSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAERE
                     RNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQALKD
                     FHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKGII
                     IHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVEGI
                     SANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDELAN
                     VLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMTPQ
                     NARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIPDD
                     FSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQVM
                     FALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGYFN
                     YTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSITL
                     KEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDIVVDKK
                     QSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLGYA
                     VFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQAV
                     ITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVVEP
                     QGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
BASE COUNT         4674 a         4972 c         4909 g         4662 t
ORIGIN      
        1 cagagcagtt gcccctgtaa ggcacggttt attgcgtcga tgatggatga tacgtatgag
       61 acggatgacg ggggaagtga agcggaacaa catgcgcagt cggcgaaaaa ggatctgact
      121 tctggttctg attcatcctc tgatgatgta aaactggatt acagaattaa attatcaggt
      181 aataagatac tgacgccact gaatatacct gattataaag aaatgataag cggtggtagt
      241 acgaagaata cagaaaaaat agattttact atcacaaata aaggcgatga ggctgaagga
      301 ctttctctgg aagtgcttga tggtaatgaa gtgatttaca gtgaacagca gaccggtaaa
      361 tattttgata agggagagca tgcctggcaa tgggatggct acagtaatca aggcatactg
      421 gatacaacta agctaaaaag caaatcacta ctcgtaagac tgattgcttt atgtggtgat
      481 atgatgataa aagttgatta tccacttcat aattcaccag aggaataaaa atgggtcgat
      541 gtaaaggtag acagaaaaca aaaaacagta gatataatat ggcgactggc agttagtgat
      601 ggagggataa aaggaagtaa tcctaagtta tccccagttc cttataacga cttggttaac
      661 ctgaccaaaa atggagttga attttattgg tctcgaaatg gaagtcgcgg cggggggatt
      721 ggtgaaaata ttgttacagc gattggtgtt tttaaagtaa atgtaaaggc tgagataaat
      781 attactccat ccatgcgaac tttttctttg atatcatcat tagttcctga ctttcaggca
      841 tccgttagtc tatcaggatt tgaaaaaata tattataact acggtgatag ttataaggat
      901 attcaagatg aattacaggc gcttcttgac gctaataaca gatataaatg ggatagtgct
      961 catgaaatgg ggcataaagt actcgatgaa tatggagagg gttctagccc tgattattcg
     1021 tggactcata aaggaacatc tactcttatg caaaaaacaa taccagggaa tgttatgccc
     1081 gctcagggag agattgatgt aatgaaatat ggaaaatatc gtcctgacat gtatactcga
     1141 ttggttgcag cagatgaaga tgttcaggga ttgatctggt taagcaggat aaaatttgat
     1201 gactagagta gttattgctt tattatttct tgccggatgt acttcgcaaa tgaagtattc
     1261 caatgtcgtc aggattagga atgtatgttc ctcagatatt aatgtgttta ttcctgagta
     1321 tgtttttgga ggtaatgtta ttaagcaaca tcatatttac ttacctgtta atgggattga
     1381 tgttttgtct tatttttata ctgaaaacat caatgatggt ttccaatatg aagaagcatt
     1441 caccgtaaat aaagatagct cgggtaattt tgattatccg tttgttatct tatcatcagg
     1501 acgtgaaaaa aaaatttggg aaaaacgatc taattgaatt atccagggac atttcaacaa
     1561 aagacgataa gaagcatggg gttgtaaagt acatgataga tagtcaggat atttgcccat
     1621 agtatgattc atatcataat caactgaaaa aatgacatta ttaatcattt gtgatttttt
     1681 gatactacca agtgaaattt gatgattaga ttaatattct cttttttatt atgcacactg
     1741 tgtgctggat gtttatctca accattctat tcttgttcca ttgtcatggt aaatacatgc
     1801 aaagtatcta ttaacatttc gacagtcaat aattcaacac attatgatgg taaggtaaga
     1861 gataattttc atcttgatat cggagagcgg aagccaattg tatactttat ttacacaggt
     1921 agagagtcag atgtttttaa taaaaaccct gtaaatattg tttctgaact tagtattttg
     1981 atatctgatg gtaacaattc taaaacttta tctggatatc atatctcctc tttattgaaa
     2041 aaatcctcat ctagtatgga gtccgatttt tcttttgaaa taaatgatcc atctatatgt
     2101 ccttaggatt ctgaaaataa aaaattctaa tctcactact cgttggtatt aatttcaata
     2161 aaaggaaaga taaatcctcc gatggacgac ttaacccaac gctactacga agccgaaatg
     2221 cgctacctgc gtgaagcggg gaaagaattt gcgcaggctt accccgaccg cgccgcgatg
     2281 ctcaatcttg ataaacccgg cgcacgcgat ccctatgtgg aacgcctgtt cgaaggcttt
     2341 gcttttttaa tgggccgcct gcgcgagaag ctggacgacg atctgccgga gctgactgaa
     2401 gggctggtca gcctgctgtg gcctcactac ctgcgaacta tcccgtcact ttccgtggtt
     2461 gaactgtcga cagatcaccg gcagatgaag cagagcgaaa cgctgagcga ttttcaggta
     2521 ttgtcgcgcc ctgttggcga gcgcagaacc cgctgtttct acagcgccac gcgggatatc
     2581 acgctgcatc cgctggcgtt gccggacgtg tcattgcaat atgaaccgga cggacgttcg
     2641 gttattcgtc tgcgctttga atgcggcccg ctggtcggtg actggtcaca gatcgattta
     2701 tcccgcctgc cgctctatct caatgccggc agtccggtgg cctgtgcgct gcaccgggct
     2761 ttaacgcttg gtacgcagca gttctggttg cgtctgccgg ggcaggatcg ccggacgctt
     2821 gacgcacact tttccccgtt gggctttgag gataatgaca ggctctggcc aaaaggcgag
     2881 tcggcgttca gcggctacca gttgctgctg gagtatttca ccttccgcga gaagtttatg
     2941 tttgtggcac tcaacgggct tgagctggtt gcatggccgg aggggataac cggctttgag
     3001 attgacgtgg tgctgaacga gaactggcct cacgacctgc cgtttgacag cgataatatc
     3061 cgtctgcact gcgtgccggt catcaatctg ttcccgctcg aagccgatcc gttacacctt
     3121 tcgccactgg aaaacgaatt tctgctacgc ccgatgcgca ttcaggacgg gcataccgag
     3181 atttattccg tcgacaacat tatttcttcg cgccataccg gcagtcaggc gtatgtcccc
     3241 ttctccagct ttcgtcaccg gggcgggatg ctgcgccacg atgcgccgga acgctattac
     3301 cacacgcggg taaagcgcgg cccttccggt ctgcatgaca cctggctgat tctgggcggt
     3361 gatgcgtttg atgctgaccg gatgctggag gatgaaacgc tctctttaag cctgaccggc
     3421 actaacgggc atcttccccg taaggcttta cagagcacgc tgctcgatag gccagttcat
     3481 gcctcacaga acgtcctgcg ggtgcgcaac ctgtgcgccc caacgcagcc ctgctatccc
     3541 ccggcgcgtg accgcttcca ctggcgggtg ctcagccacc ttggttctaa ctttttatcg
     3601 atgatggata acgccgaaat tctgcgcgga acgctggcgc tgtacgactg gaccgaaagc
     3661 gagatgaacc gccgtcgtct ggaggcgatt gtcgacgttc agcacagcct gatacagcgt
     3721 tttgaaagag gctttctgct gcgtggcgtg gatattcagg tgacgctgga cagtaacggc
     3781 tttgccggag aaggtgatat cacgctgttt ggtgaactgc tgcaccgctt tttcgcactg
     3841 tatgccgata tccacttatt cactcaactg accttaattc tgcaacctac aggaaagtgc
     3901 ctgcaatgga cagagcatca cagccagcgc gtaccgggct gaccgaccgg ctggccccgg
     3961 atattgcgcg gatcaatttt taccgttttt gccagttgct ggaacagagc cagcagaacg
     4021 cgccgctggg cagtaccgac aaccccgcca ccgatgcggt gcgctttcgt ccccatcccg
     4081 gaatgggctt tccggtcagc gagttaaaga acgttgaacg tgacgcgggc aatcccgacg
     4141 tgccacccac ggtgcgcacc acctttctcg gcctgtacgg cgtggattcg ccgctgccaa
     4201 ccgcctatct ggactacatt acccagcgtc atgacggaca tgacgcagtg atggcgtttc
     4261 tcgatatttt taaccatcgc tttatcaccc agtattaccg catctggcgc aaatataact
     4321 acccggcgtc gtttgaagca ggtgctatgg atgacatttc ccgttgtctg ctgggactca
     4381 tcgggctggg tattcccggc agcgagaacc atatcgccac gccggtatcc cgttttctgg
     4441 ccctgctcag cgtgatgcgc ctgccgacgc gcacggcgga aggcgtcacg gcactggtgg
     4501 gactgcttgc cccgctgacg aaagcgacgg tggtgcctca cgatccgcag cctgttattc
     4561 tcccggcccc tgcgggtctg tcaaaaaatt cacgcatcag cctgaaaacg cgaacgctgc
     4621 tggggcgtac cggcaccgac gtcaacagcc agttgctgct gaaactgtat accgaagatg
     4681 ccgcagaagc acgtggctgg ctacctggcg ggcagctgca cagcgatttg ctggtgctgc
     4741 tgcgcgttta tctgggctgg cgctgtcagg cccgtctgca actgacgctg cccgtcagcc
     4801 tgttgcctgc tgcccggctg ggaaaacagc gcgtacagat tagccgtacc ggcatccttc
     4861 gcgcctcctt tgccgcgccc gcgaccggaa cagtgacggt cagtctgggg cgttatcagg
     4921 gattaatacc cgctttatcc attcgtaaca gggagagtat gactcatgtt tcctattcgt
     4981 tttaaacgtc cggcgctgct ctgtatggcg atgctgacgg ttgttctgag cggctgcggg
     5041 ctgattcaga aagtggtgga tgaatcgaaa agcgtggcct cagccgtttt ctacaagcaa
     5101 atcaaaatac tgcatctcga tttcttctcc cgaagtgccc tgaatacgga tgcggaagat
     5161 acgccgcttt ccacgatggt gcatgtctgg caactgaaaa cccgcgaaga ttttgacaag
     5221 gcggattatg acaccctgtt tatgcaggaa gagaagacgc tggagaagga cgtactggca
     5281 aaacacaccg tctgggtaaa accggaaggc acggcatccc tgaatgtgcc gctggataaa
     5341 gagacgcagt ttgtcgccat tattgggcag ttttatcacc ctgatgaaaa aagcgacagc
     5401 tggcgtctgg tgatcaaaag ggacgaactg gaggccgaca agccgcgctc gattgaactg
     5461 atgagaagcg acctgcgact gctgcctctc aaggataaat aaccgatgtc gacaccttcc
     5521 ttatatgaaa tgctgacgtt cagtttcagc ggcgagttac cactggaaca ggtcagcgag
     5581 cgggatcagc ttatcctttc cgtgatggac aatatgcaac gcattatcaa ctgtcgggca
     5641 ggcacgctgg cgcatttgcc ggattacggt ctgccggatc tcagtctgat ccatcagggg
     5701 atggctgcgg gtattcatgg cctgatgcgt cagattgaag aaacgctgct gcgttatgaa
     5761 ccacgcctga gtcagataca ggtggagtta ctcccccagc cccgtccggg gcatcttaat
     5821 tacctgatcc acgcgcagct tcccgatacc ggctggctac gctttgatgg cgtgttttct
     5881 ccggaaggac gaattgttct gcgtcatctc aaacaacaag agcgggcgta ctgatggcaa
     5941 gtaacgcgaa ttttatcagc cagttcgtca tgggcgggga tccctgtact tataaggaat
     6001 ccggtgaact acaggctgaa atgagtaaac tgactcaccc ggcccgaccg gatgtggact
     6061 ggcgtcaggt ggaaaaactc agcctcgcgc tgttccggca aaatggcgtg gaactacaga
     6121 cgctggtctg ttacgtactg gcaataacca gacgacaggg gctggcaggg atggctgacg
     6181 gactcggttc actggacata ttgctccagc gctgggctga cttttggccg gtacaggtac
     6241 attccagaat atcactgctc agttgggtca cagaaaaaat gcagcaggca ctgagagcgc
     6301 tggatattca gtatcaggat ctgccgcaaa tctaccgttg tgtacagcat ctttctgcca
     6361 tcgaaaccac actgcaacag tgtgaactgt ggcatatgac gaaactggat gtgctggccg
     6421 ggcagtttcg taatgcggcg ctacgtctgg agcggctggc accacaggga gcggaaacca
     6481 ctatcactcc ccctgaatta ccccgccggg aaatgaatca accgaaaaag tcagaagaaa
     6541 gtccacagcc ggtttttgca accagacccg ctcagcaaaa cgataaggat gccagtccac
     6601 ccgtgccatc ccctgaaatc tcccggcagc gggcatggcc gatatttatg gccggaatgg
     6661 ttgtgatggc ctgtctcggc ggaacaggat tatggggctg gttgcagctt aatcagccgg
     6721 acgcgctaat ccagcgaata caactgtctg tcatgccatt accgcagtcg ctggagagcg
     6781 gcgaactggc aaagctggat gtaaaggata aggcgctgct ggctcaggac agaacaattg
     6841 cggcaagtca gatgcaactg gagcagttaa acaaattgcc tgcccgctgg ccgctggagc
     6901 agggatatcg ccagctacgc cagcttgatg ccctgtggcc ggataatcct caggtcaggg
     6961 cgctgaacgc gcagtggcgt aaacagcggg aactgagcgc cctgtccaca gaggcactga
     7021 atggctacgc acaggcgcag agccagctac agcgcctgtc ggcgcagctg gatgcactgg
     7081 atgagcgtaa ggggcgatat cttaccggtt cggaactaaa aacggcggtg tatggcatcc
     7141 ggcagtcgtt aaaggagccg ccgctggaag aactgcttcg gcaactggaa gaacaaaaac
     7201 agaccggaga gatctcgccg acgctgttga cgcaaattga tacccggtta aatcagttgt
     7261 tgaatcgcta tgtaatttta ctggatacga aggtggaaca aagtcagtaa acgtcggggg
     7321 atttatgcac caataaggga tatgtggcct tgccattttt taaaaggagt ctttgtgtac
     7381 aaaaaagaga ggaacaggcc attcgaaacg cgtgaagagg ttctggcata tcataagcgg
     7441 gaaaaaatac cctgtctgga gtgtggcaag ttactcattt ttttaccgcg ccatatctgg
     7501 tttgtgcatg gaatacgcgc tgacgaatat cgtgaaaaat ggaacatacc gaagcacatc
     7561 gcactggcag ggatttcata tctggagaaa caaagtgaat atatgaaggc gagaatagca
     7621 aagggaaagc tggatcccgc agaacaaata gccatgatga gaataaagag aaggcaacta
     7681 aagtcagatc ctgactggcg taatcgctcc ggttctatat tgaagaaaca atatgcccga
     7741 cgagatatcc ttaaaaacag gatatgggaa agatctccca gcataaaaag tgtctctgtg
     7801 gagttgaaaa cagaagctat aaggcgaatg aaaaacagga aggtcgccgg ggaaaaagtc
     7861 caggatattg ccgatgacct gaacgtatct atcagcagat tgtatcactg gctaaagatg
     7921 taggcgctca tgtgctctca atgccttcaa taaagccgct atgcgcgaac cgcgcacatc
     7981 tggtagactg ttctcagtga gtcaagcaaa ctgcctactt ttctaacttt taatcatgaa
     8041 taaccagcgc cttgagtata gctggttcga atatactcgt tgattcacat cagataatga
     8101 gtgtataatt cggtagatag cttgggttcg ggaacagtga atgccttaag tttcaaaagg
     8161 ttagattaac ctcttgactg tggcccccgc aaccaattat tgaacaccct aacgggtgtt
     8221 tttttgtttc tgctctccca taaaaaaagc gccattcagc gcctttttat catccccttc
     8281 tcgccagcgt cgccccatcg gcaaaatacg ctttaatccc cgcaagaata gactccgcaa
     8341 cttcctgctg gaaagtcgcc gttttcagtt tacgctcttc ctcaacgtta ctgataaacg
     8401 ccgtttcgac cagaatggag ggaatatctg gtgcctttag tacggcaaac ccggcctgtt
     8461 caacttgatt tttatgcagc ttgttgattt taccgagctt attcagcacc gctttaccaa
     8521 acttcaggct gtcggcaatg gtcagcgact gcaccatatc gaacatggtg tggtcgacat
     8581 agcggtcacc gcttttgctc acgccaccaa tcaagtcgga ggcgttctgg gtttgtgcca
     8641 gatattttgc cgcagtactg gttgcacctt tggttgagag cgcaaacacc gaggaaccgc
     8701 tcggctggcg actggtaaag gcgtcggcat ggatagagac aaacaggtca gcacgctgtt
     8761 tctgagcttt tgctacgcgc acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt
     8821 acaccttcat attgccctct ttctcgatca gagagcgcaa acggcgagct atttgcaata
     8881 ccacatcttt ttcgcgcgtt ttgtatttcc ccaccgcacc ggagtcttcg ccaccgtggc
     8941 cagggtcaag cataatgaca atcggacgat cgcgccctgc tttacctggt tgtggaccac
     9001 tttgcgctgg cggcacctgc ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca
     9061 gcagcgggtc ctgcatatcc tgtgcattgg ccggatagag gtccatcacc agacgctctt
     9121 taaacccggc gaccggcgca agggcaaaca gctgcggttt tacgttttgc tttaattcaa
     9181 aaaccatccg tacggtttgc gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt
     9241 cgtctgcacg aatttgtgcc gccatcccct tgagtaccga gttcaggttt acatcttcga
     9301 tatccaccac cacgcgttcc gggttactca acgcgaactg cttatatttc agctgacgat
     9361 ttgattctac cgtcacacgg gtgtagctgg acgcaggcca gacgcgcacc gccacgacct
     9421 ggctgaccgc agccagactg acctgactta cgctcaatag ccacatggca cccgcgcctt
     9481 gcagtaaacg acgacggctg attgcagtgt tggatcctga catgcctctc ccgagcaaaa
     9541 gaaatctaag ctgtgtaaca agtaaacgac taatttgacc ggaaacttta gcgaaagacg
     9601 cataatctgt catctaataa acggtaaaca ttctttttat attcacggca ttactgataa
     9661 aaaagtcgct ctcgcataaa atttacactt gcaccctgcg aaaaaacaga ataaaaatac
     9721 actaatttcg aataatcatg caaagaggtg tgccgtggta aaggaacgta aaaccgagtt
     9781 ggtcgaggga ttccgccatt cggttcccta tatcaatacc caccggggaa aaacgtttgt
     9841 catcatgctc ggcggtgaag ccattgagca tgagaatttc tccagtatcg ttaatgatat
     9901 cgggttgttg cacagcctcg gcatccgtct ggtggtggtc tatggcgcac gtccgcagat
     9961 cgacgcaaat ctggccgcgc atcaccacga accgctgtat cacaagaata tacgtgtgac
    10021 cgacgccaaa acactggaac tggtgaagca ggctgcggga acattgcaac tggacatcac
    10081 tgctcgcctg tcgatgagtc tcaataacac gccgctacag ggcgcgcata tcaacgtcgt
    10141 cagtggcaat tttattattg cccagccgct gggcgtcgat gacggcgtgg attactgcca
    10201 tagcgggcgt atccggcgga ttgatgaaga cgcgatccat cgtcaactgg acagcggtgc
    10261 aatagtgcta atggggccgg tcgctgtttc agtcactggc gagagcttta atctgacctc
    10321 ggaagagatt gccactcaac tggccatcaa actgaaagct gaaaagatga ttggtttttg
    10381 ctcttcccaa ggcgtcacta atgacgacgg tgatattgtc tccgaacttt tccctaacga
    10441 agcgcaagca cgggtagaag cccaggaaga gaaaggcgat tacaactccg gtacggtgcg
    10501 ctttttgcgt ggcgcagtga aagcctgccg cagcggcgtg cgtcgctgtc atttaatcag
    10561 ttatcaggaa gatggcgcgc tgttgcaaga gttgttctca cgcgacggta tcggtacgca
    10621 gattgtgatg gaaagcgccg agcagattcg tcgcgcaaca atcaacgata ttggcggtat
    10681 tctggagttg attcgcccgc tggagcagca aggtattctg gtgcgccgtt ctcgcgagca
    10741 gctggagatg gaaatcgata aattcaccat tattcagcgc gataacacga ctattgcctg
    10801 cgccgcgctc tatccgttcc cggaagagaa gattggggaa atggcctgtg tggcagttca
    10861 cccggattac cgcagttcat caaggggtga agttctgctg gaacgcattg ccgctcaggc
    10921 gaagcagagc ggcttaagca aattgtttgt gctgaccacg cgcagtattc actggttcca
    10981 ggaacgtgga tttaccccag tggatattga tttactgccc gagagcaaaa agcagttgta
    11041 caactaccag cgtaaatcca aagtgttgat ggcggattta gggtaagcga tgaaaatcgt
    11101 cggatgcgac atgcgtaaca ctcgtacgtc gcatccggca attacgttta ttcccgtgaa
    11161 ctaaacaacg ccgccagacc actgcgccgc tcagtacgag tggcgattgc cgcacttaat
    11221 atgcgctcat cggcatacag cgacagacgg cgacgcgcgc gggtcaccgc ggtataaacc
    11281 agctctcgcg ttactaccgg cgtgcgttgg ctcggcaaaa tcaacgccgc atggtcgaac
    11341 tccgatccct gcgatttatg taccgtcatc gcccacgtag tttcgtgctc tggcaagcga
    11401 ctcggttgca cagacttaat attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc
    11461 ccgcgatcca gcgcaatacc gatatcgcca ttaaacaacc caagcgcgct gtcattacgg
    11521 gcaatcatca ccggtcgacc ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc
    11581 ttctgttgca taaactgctc aattcgctca ttcagtccag ccacgccaaa cggcccttcc
    11641 cgcagggcgc acaaaagctg gtactcattg aacgcctgaa tgattaaatc cggctcggca
    11701 cgcgcttgca gcagatccag ataacgtccg taacccgcaa gagcttcctc aagcatcgca
    11761 atataatctt cgccgctctg taaaagccgt ttttcgatat cagtaaaatc ctgctgaaaa
    11821 acggttttca ctgccgtttt atcaccacgg ttgatcgccg cagctaactg accaatgcca
    11881 gaatcgctgc cgaaacgata gcttttttgc agcaggcaga gactgtcgcg caaagatgcc
    11941 gcttctgtgc cggttcctgc cggaacgtga gtccccgtca ggcggcttag ctgcctggca
    12001 cgctcggcgg taaagcccgc gttggcataa gcgcagatat cgcccagcac agccccagcc
    12061 tcaaccgagg ccagttgatc acgatcgccg agaaagatca ctcgcgcatg atcgggcaag
    12121 gcgtcgatca gtctcgacat cataggcaga tcgatcattg acgcttcatc taccaccagc
    12181 acatcaagat gcagcgggtt accggcatga tgacgtaaac gctggctacc cggctgcgcg
    12241 cccagcaatc ggtgcaaagt gctggcatct tccggaatgc gtttcttttg ttcatcggtc
    12301 agcggtaact gtcgcaaagc cttgccgaga gattcggtta agcgcgcggc agctttaccc
    12361 gttggtgcag ccagacggat acggcagcgt tcgccgtcgg ccatttgaat taacgctgcc
    12421 agcaacttcg ctacggtggt cgttttaccg gtgccagggc cgccggaaat caccgagatc
    12481 cgccgcgtca gcgccactgc cgccgcaact ttttgccagt taatttcatc gcttactgga
    12541 aaaagtttgt ccagggtttg cgccagtaga gcttcatcaa cctcaatggc atgattcact
    12601 tcgttgaaaa agcgtgccac tgtgcgctcg ttacaccaca tgcgattcaa ataaagacga
    12661 tcgccacaga ggatcatcgg cgtgggttca tctcccctgc tgaccgcttg agaagccagc
    12721 aagcattctt cccaattttg tagctcaccg atttcactga cacaggtcgc caacagcgga
    12781 tgcgacgcct cgttattttc cagtcgtgaa agcggcaaac aaacgtgtcc ctctccggca
    12841 tcatgactta acagtgccgc cgcgagggtg acggcaggat gttcatctcc cgccacggtc
    12901 agggcaaatt gcacatccag cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc
    12961 ttttgcaatt tcattacgcc tcctccaggg tcataccggc aaacatctca tccatcaggg
    13021 caatcaaccc ggcgttgggt cgggttgtgt aaatcccctg ttgcggatgt tctttatcaa
    13081 cgccacgcag gaacagataa ataacgccgc caaagtggtg ctcatagtcg taatcagcaa
    13141 tgcgatggcg cagataacga tgcagcgcca gggtataaag ctgatattgc agatcatagc
    13201 ggtgtgcctg catcgctgct gccatagcct gttgggtgta agccgaactg tcttcaccca
    13261 accagttgga tttatagtcg agcaggtaat aacgcccttc gtggcggaac accaggtcga
    13321 taaagccttt taacatgcca cgtacctgca tgaactccag cggcgggcag cctgcggata
    13381 gcgggtcaaa ctggcggatt aacgtatcaa gctgactggc gataagcggt tcactaatcg
    13441 gcagataaaa ctccatctcc acctgtttat tgcgggcgga aagttgactc aggcttacgc
    13501 cggtttcatt gagaggtgcc tggaggacag ccgtgatcca ctcggtcaat accggttccc
    13561 actgcgattc aaagccgccg agttccagtt tttcccgcac ccagttcggg tcaaccggct
    13621 gggtaaaatc caggtcttca aacaaactgt gcaagaacgt ccccggtgac gcaccgcgcg
    13681 gaaactgatg tggtgttaac gtcggttctt caacgacgct ggcaacgcct gcagcatcga
    13741 catccagccg aggcatcaaa tcctgggcga taccgtgacc acgctgttgc aaaccagagt
    13801 agctggtgac gcgccagtta tcgccgggca atcgttgtaa cgtcttcgca ttcagctctg
    13861 ctgtagaaac atcattaacc tgccagggtt ggttatcacc aatttgtgcc gtttgccagg
    13921 caatatcatc atcgcacaat gcttcaatac aggtgcgaag ccctgccgca tcttgcggtt
    13981 cccctttttg cagcaaacgc ccgagcgcac tttggtggac gtcggtgtca ccttttttat
    14041 cgccacgacg gcgcaccagc ggtgcaacgc cgagactaca atgccaaacc gaacgcgtca
    14101 gcgccacgta aagcaaacgc agatcttccg ccagacgttc ggcctccgcg aggtcgacgc
    14161 tttctggcgc agcattaaga tccagaactg cctcaaacga gtggcgatcg tgataaaacg
    14221 cctgctcctg gacgcggaaa ttggtgataa acggcagcca gaccaatgga tattccagcc
    14281 ctttcgattt gtggatcgtg acaatctgca ccagatgttt atcactttcg agacgcattt
    14341 gttggctgga ggcattactg tctggctcga ggatatgttg cgataaccag cgtaccagcg
    14401 catgttcact ttccagctgc gttccggctt cttgtagcag ttcgctgata tgcaagatat
    14461 cggtaagacg ccgctcaccg cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg
    14521 acatcagcgc ccgcagcatc ggcataacgc cacgtttgcg ccagatttgc cgataaccat
    14581 cgaactcttc aactaccgca tcccacgcat gttcgtcatt attcagcgtt tcgatatcca
    14641 gcgcgttcag ccccatcatt gacgttgcca gcgcactacg cagggtgttc tcacgttcgg
    14701 gcgtcatcac cgcctgcaac aaccaaagca tttcctgcgc ttccagagtt tcaaaaacac
    14761 tgtcgcggtt cgaaaggtaa acggaaggga tttccagcaa cgttaaggca tcgcgcacct
    14821 gggcggcctc ctggcggctg cgcaccagca cactgatgtc cgaagcacgc accggacgcg
    14881 cgtcgtcgcc gttcatcagc aacgcttcgc ccctctgtcc ggcttgtagc cagtcgcgga
    14941 tttgcgcagc acatacctgc gccatggtac tttgataatc gccaacgccg cagctttcgc
    15001 cttccatcag ccacattttc atcgcaggct gcgtttcacc tttaaataca aaacgtaacg
    15061 cctgattttt cccggctgat ttcactggaa taaacggtat ttcgcgaaac atgaacgcgt
    15121 catcagtctg gctgaaaagc ttattcacgc tgttcaccat tcctggtgcg gaacgccagt
    15181 tggtgtctaa agtgtagtgg gcgtgaactt cgctacgcgc cttcatataa gtgaagatat
    15241 ccgcaccccg gaatgcatat atggcctgct tcgggtcgcc aattagcaac aatgcggttt
    15301 ccggctgatg gtgccagata cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa
    15361 attcatcgat cattgccacc gggaatcgcg tacggatcgc cgctgccaac acctcaccgc
    15421 tttcgctacg cagcgcggaa tcgagccgac ttaacatgtc atcaaaaccc aattcgccac
    15481 ggcggcgttt ttcacgcgct actgtttcgc ggatctcagc caatgcgcgg gtgatcacca
    15541 gatcgcggat cgacaatggt tctgcaagca gttgatcgat cgcctcaaac agtggatgtc
    15601 gcggggtttc ccccccggcc ttcgtgcgat cttctaagaa acgctgggag aatttttcca
    15661 gcgactccgg caactgataa ctgtttgtct cttcttctgc ccaggcgctg atcttgtcga
    15721 tccatttagc ctgattgcta cggttaaact tgcgtcgatc aataccagaa gattcgatca
    15781 gcgcatccag ttcacccact gcgtcgcgcc actgctgttt taccgtatca atacgcgcca
    15841 caatttgcgc gtgacgggaa gccagcgttt catcatcggg cggcggtgct ttgataaccg
    15901 gcgcttcgcc ttgcagataa cgattaatat cgcgcagcaa cgcctgcggc cctttccagg
    15961 tttcaaagac gacctgggct atttcacgcg gcagcgggta gcagtggcga cgccagaaat
    16021 cggcgcaggc ctggtagcgt agcagagact catcttcaat cagctgctgc tcaaacagca
    16081 tgccggattc aaaggcattc aggttgagca tgcgctggca aaagccgtga atagtaaaga
    16141 ctgccgcttc atccatctgc cgttcggcta acaacaacca ctgcgcggct tgcgctttat
    16201 cgtcgatctc ttccagcagg cgttcgtaca gtggattgtc ggtggtttca cgcagacagg
    16261 cgatgcgcaa ctcgtggata ttgctacgga tacgaccgcg caattctgcc gtggcagcct
    16321 cggtaaaggt gaccaccagc agttcttcaa cggtcagcgg gcggggaaag gcggcggaac
    16381 cgcctagtcc aagtaacagg cgcaaataga gcgccgcaat cgtaaaggtt ttgcctgtgc
    16441 cggcagaggc ttcaatcagg cgctcaccct gtaagggcaa gcgcaaagga tctagtgtct
    16501 cggcgacatc actcattctt ttcactcatc aggggcattg tttgctgcaa cgcgctgacg
    16561 ttctcccaca ctttccagcc ttcagggtgt acatattcgg ctttcccgtt ctggctgccg
    16621 gaaatctgcg acagaatagc catgccttgc ggctcgacca ccgcctgatg gaagaaatcg
    16681 gccagttttt gcggcgtcag cagtttaatc tgggccacga ttttatcacg cgaatcgaag
    16741 cgcatattgc cgcgatcgaa atctttactt aacttcgatg cttcttcgcc gagcgtttgc
    16801 ggtgcctgca gcatctgggt aattaccgcc tgctggattt gcgcaaactc atctggcttc
    16861 atcgctcgca attttgcctc tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat
    16921 gaaggctgtt tatcattgct ttgcaaaagg aagcccatgc cccactgacg ccccacgctc
    16981 attggaaacg caaacacggc atagcccaat tgttcttcgg tacgcaactg attgtagaac
    17041 cacggctgta cgatctgccc caacagagag ctataggctg agctggtgta ttcatcgtag
    17101 ccagtcggta caaataccgc tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag
    17161 atgacggatt gttttttatc gaccactata tctttgtttc gacaccactc tgaaccatca
    17221 gcgcccaact gtttttgcac atcgcgtgcc agggttgttg cctggtcctc ggtcatgttg
    17281 ccgataacca taaactctgg tcgagccccc gattttaagg cgtcgcgata ggccagcacc
    17341 tctttcaacg taatggaggg caaaattttg cgccgttcat ctcgcgagaa gtaaggtacc
    17401 tgggagagca tctgcgcggg cataatcgcc tgctcgaacg ctttgccctt ttctgcggaa
    17461 tccatcatct ggttatacca ggacttcgcc tgctcaagct gatcttccgt agcggtatag
    17521 ttaaagtagc cctcgagcaa tgcctggaac agctgcggca ggcgctgggt gtaaccatta
    17581 gcattaacca taaggccgtt gttagcgttg gtggaaaaac ttatgccacc aaccgacgcc
    17641 tggttgctta actgatcaag cgccagccct gcgagataat cattgagcgc aaacatcacc
    17701 tgattgcggg cgctgtccat ggctttcgga ttacgcaaaa tcaggctgac atcggctttg
    17761 ggctcgctgg caaaataacg gcttggcgca tacactactc gcagattcga ctcatcaaca
    17821 atcagctctg gatggtcgta tttcttctct gacttaatca gcgagaaatc gtcaggaata
    17881 taagggttaa gctctggcaa agagagcgca atgtcggcag cttttttctg ccagtcagcg
    17941 aaagtttgtg cgctaatttt atcgacctga tacggcgcat cgacaaaata agccgtttta
    18001 ttgtgcggct ctttcgggct gatataccag atacgcgcat tctgcggcgt catcatcgcc
    18061 agacgttcct ttactgcttt agcatcgtac cgatcggcaa tattgactgc atccagcgta
    18121 tgctcaacag gaacgcgaat catggtatct gccagccatt cgacgtaatc catatcacgg
    18181 gtgatcgacg gataacggaa gtcgatatcc agcacattcg ccagttcatc gaagtattgt
    18241 ttatcaatgc ctttttcacg taacagattg agatagctaa aaattgccgc cacaacctga
    18301 tcgcgattag ccaggccttt atcggttaaa gacgcagaga tcgctaatac gccgctgttg
    18361 ccgttgacga taggatcgga gttggcgcta atgccctcaa ctaatccctg cttttgcagc
    18421 cagtcggaaa gtgtacctgg gctgcgatta ccaatcagat aggtaatcaa ttcatccgtt
    18481 ttactacgga acttcgctga gttgttatcg atgcgaaact caacgcgcag aactttacgc
    18541 ggcagtgccg ggacgtaatg aatgataatg cccttttgcg cgtcggtgac taccggcacg
    18601 gtgatttccg gttttttgct ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt
    18661 tttgccaact ccggcagcgg tttattgctg taaataaccg ccttcatcag attggcggaa
    18721 tagtacttct cgtggaaatc tttcagcgcc tgctgcaccg gattaccagg tttgtcgctt
    18781 aaagtttcga ggttaccacc agaaaacttt gaaccggggt gtgccgggtt aatggtttct
    18841 gcgctgacct gcgccatgcg catcccgtca cgcgtacgcg ccatggttaa ttcggcattc
    18901 actgcattac gttcacgttc ggcgtatttc ttgtcgagca agggttctgc aatagcgtcc
    18961 gccaggcggt ctaccgcacc gggcaaggcg tcgttctcaa cttccagata gaaagccgtg
    19021 cgatacggtg ccgtgctggc attgtgactg ccgccgtgca ttttgagata ttcggccaga
    19081 ctgtcagcct gcgggtactt tttcgacccc atcagactca tatgttcaag gtaatgtgcc
    19141 agcccctggt acgcctcggg atcttccagc gacccaacgg gcaccaccag cgccgagagc
    19201 gatttaaccg cctgcgg
//
LOCUS       DAEVZA010000096        19183 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEVZA010000096 DAEVZA010000000
VERSION     DAEVZA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.096"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            56..1224
                     /locus_tag="LAF92_003426"
     CDS             join(56..305,305..1224)
                     /locus_tag="LAF92_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI9792379.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGAR
                     SIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEIR
                     SKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEWI
                     PVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
     gene            complement(1232..1450)
                     /locus_tag="LAF92_003427"
                     /pseudo
     CDS             complement(1232..1450)
                     /locus_tag="LAF92_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(1692..3098)
                     /gene="gndA"
                     /locus_tag="LAF92_003428"
     CDS             complement(1692..3098)
                     /gene="gndA"
                     /locus_tag="LAF92_003428"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBI9792380.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGTGTDAAIDSLKPYLEKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNDELSSYLIDITKDIFTKKDEGGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPKIANLLLAPY
                     FKKIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(3240..4256)
                     /gene="galE"
                     /locus_tag="LAF92_003429"
     CDS             complement(3240..4256)
                     /gene="galE"
                     /locus_tag="LAF92_003429"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBI9792381.1"
                     /translation="MSVLVTGGAGYIGSHTVLSLLERGYEVVVLDNHSNSSYEALCRV
                     KLLTKRDITYYRGDVSNRALLQKIFTENSIKQVMHFAGLKSVSESVQFPLEYYNNNVT
                     ASIVLLQEMMAHNVHELIFSSSATVYGNPETVPLKESSKIGGTTNPYGASKLMTEQIL
                     NDIAKSYPQFDITILRYFNPVGAHPSGFIGEDPNGIPCNLVPYICQVAVGKLPELLIY
                     GHDYPTHDGTGVRDYIHVMDLAEGHIAALQNKAQGSNCRTYNLGTGKGYSVLDLIAAF
                     ERQSHKTIPHRFVDRRPGDVAACWANPSLAEQELKWSASRDLDIMMRDSWNWQQKNPC
                     GYGS"
     gene            complement(4298..5419)
                     /locus_tag="LAF92_003430"
     CDS             complement(4298..5419)
                     /locus_tag="LAF92_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBI9792382.1"
                     /translation="MRILLIGNQSGTIVLFRKRLIEKLVSKNCVVHTLTMDNDAENFK
                     RIINMGAHPAKYEFSRSGTNPLSDLFNTIKLSRIIKKIEPDIVFCFFPKPVIFGTLAA
                     RIIGITKIDVLLEGLGYCYTRHATPDGIKKKILRTVQTTLYRLALPLCRKVIFLNPDD
                     HKELLLQNNISVKSTKIIGGIGVDLSEYNYSPAPVKPVHFTMISRLLVEKGIREFVHA
                     AEKVKLRYPEVKFSIAGAFDDNPGGITSSEFEGWVKNGSIEFLGQVSDIKSHLINSSV
                     FVLPSYREGVPRSTQEAMAVGRAIITTDVPGCRETVIDGRNGFLIPPWNAEALAEKMF
                     VFIEQPDLILTMGRTSREIALEKFDQEQVCDRLVSMILS"
     gene            complement(5560..6321)
                     /locus_tag="LAF92_003431"
     CDS             complement(5560..6321)
                     /locus_tag="LAF92_003431"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBI9792383.1"
                     /translation="MNEKFGVSGLLALNNIDNYTRVAIDSILNQSRPVDELVIVVNGC
                     HCESIIETLSEWYGNLTNVRFVKCGVEQLSFALNLGLLHCNYKYVARMDADDISHYNR
                     IEVQLNYIESKNLDMIGCAINKIDASDRIIATVNYPVGSNINKKLPFTNPFCHPSVMY
                     KKETIINACGYNAGFKSEDYDLWLRLSRMSVRWDNITDVLFSYRVHSSTAQRKPLAYA
                     EQAGLMVREMLINKSFFFLIASLISVMKFFIRGKN"
     gene            complement(6364..7266)
                     /locus_tag="LAF92_003432"
     CDS             complement(6364..7266)
                     /locus_tag="LAF92_003432"
                     /inference="COORDINATES: protein motif:HMM:NF013681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HBI9792384.1"
                     /translation="MMMIIRFSGGLGNQLFQFAAAKVLEVKFGGSIVVDDSYYDNQPS
                     KDTFRKLEIFQFNVQYTRKSNCKEKSNTRKKVLILKILTRIPWYSNSSAFRKITRLIH
                     IYNEDAFTYHMNARENDYVIGYFQNYSLLKENINIIRQQFTLTPEVDAEMRRLDSYRT
                     ISQHDNTIAVHIRRGDYVTNVNASAFHGICDIEYYKKSIELITSRIKDPKFVFFSDDI
                     AWVKEAFSGVSDAYFVENPSSTSSAVDMYLMSLCKHNIIANSTYSWWGAVLNANPEKI
                     VICPERWTLNDSIGQLYVDGWIKL"
     gene            complement(7302..8357)
                     /locus_tag="LAF92_003433"
     CDS             complement(7302..8357)
                     /locus_tag="LAF92_003433"
                     /inference="COORDINATES: protein motif:HMM:NF026247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HBI9792385.1"
                     /translation="MYIILLIYLFFCSFVNNKYLATFNVFATFAVMSGWYFPAYDWIN
                     YYDFYTRIDNIELFNNVFEPGFTALMKVCAWMGFEYHSVYMLSNVIIYFFVYRYCVRF
                     RYSGFVFFFIFALFGFVLFAEQVRQGIALAIILFASQKRKFILYALVACLFHYSAIFS
                     IGLYFIISSKEEKRRKMMLIFSCVVLVSLAFLVFDFNSTIVYFITQKISNYLNSNIFS
                     PSFALGFLLYFILSLLCLYKRKENIVNKKWQVFCFGVVLLGIPFPVFNRFTYFCYPYL
                     IPDIERLFSKYISRGILISIVVFVIGLRIVVSPIYLPLMSDYRFHLPGVTEPPNYNFI
                     MQGRCEILIRNGINDFC"
     gene            complement(8509..9234)
                     /locus_tag="LAF92_003434"
     CDS             complement(8509..9234)
                     /locus_tag="LAF92_003434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792386.1"
                     /translation="MNKNKMIPLLITSCVVSNAPLTVLSDSNKRLQSTMNALFMLSEY
                     CREVPIVICDGSGYDFSDERELLENIFQNIEFLNFHNDSEGVKRFGKGYGEGQIVNFA
                     LANSEILKKYDAFAKCTSKLWLSNYFECIKVFSGRAAFLTAPFFSPDKVDTRFYIISK
                     DFYLEKFSSIHTRVDDLNGRHLEHVFFEVIDNMPYRQVIGREIADIYGHSGSVGIHFK
                     PSKIKNFIKKMKVMFAVILNLKV"
     gene            complement(9221..10474)
                     /locus_tag="LAF92_003435"
     CDS             complement(9221..10474)
                     /locus_tag="LAF92_003435"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HBI9792387.1"
                     /translation="MNILKNSFWNIGGFIIPSLIAIPAMGIVARLLGVELFGLFTIFF
                     TIFGFASILDAGLSRAVIREVSMHREDTHRLNVILSTSLVIILVISILGALLITLNAT
                     KIAWVFNVSAQHFTDVVRSLRIISISFPFLLFSQIYTGYLEGLENFKSINLQKTVTGS
                     ILAISPVAFIACDASLMSGVLGLTLARIISFAINHYYIRRYAKINFFFFDKTICKCLL
                     SYGGWVSVSNIISPLILYCDRFILSNMLGAKNVAFYTAPSEIVNRISIFPNAISRVLF
                     PLFSHSLNKGEATRQLRHGYLLVGSSAFLIILPFLMFPSFILDMWIGTEYGTNSVTIL
                     LILLFGMFVNSVAQVTFAEIQAKGYFKATAIIHIIELVPYFCFLYFAIKYYGVIGVAV
                     ASTFRVIVDMALLLIFRWFIQNEQK"
     gene            complement(10486..11031)
                     /gene="rfbC"
                     /locus_tag="LAF92_003436"
     CDS             complement(10486..11031)
                     /gene="rfbC"
                     /locus_tag="LAF92_003436"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013509735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBI9792388.1"
                     /translation="MNVIDTTINDVKLIEPNIFSDSRGFFMETWNEKKFEQTIGKTQF
                     VQDNHSKSCKGTLRGLHYQLVNTQGKLVRVVSGVVYDVVVDLRKNSSTFGKWAGFYLS
                     GENKRQLWIPGGLAHGFYVISKVAEFVYKCTDFYNPMAEQTLLWNDPQLAIKWPLEGK
                     PLLSEKDNLGLLFVDAPKFDY"
     gene            complement(11117..12496)
                     /locus_tag="LAF92_003437"
     CDS             complement(11117..12496)
                     /locus_tag="LAF92_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBI9792389.1"
                     /translation="MSKLSCFKTYDIRGRINHDITTEIAWKIGKAFGLYTKARSVVVG
                     GDCRLTSESLKIALANGLREAGVDVIDIGMVGTEEIYFATSYLKLDGGIEVTASHNPI
                     DYNGMKIVGRDSKPISCESGLYEIKLLAETLKIDESKNEFKGKYKRISLKQEYIDHIL
                     SFINISNLRPLKLVINSGNGAAGPVIDCLEKRFQEQSIPITIIKVNNEPDGSFPHGIP
                     NPLLPECRQDTHDAVVEHGADLGIAFDGDFDRCFFFDSNGRFIEGYYIVGLLAASFLK
                     KEPGAKIIHDPRLLFNTIDIVRKAGGIPILSKTGHAFIKDRMRAENAIYGGEMSAHHY
                     FRDFFYCDSGMIPWLLVIEMLSIEKVGLNQLIDESIAAWPASGEINRNIEDAQRCIKD
                     LCDCYSGSAQHVDYTDGVSMEFAQWRFNIRVSNTESLVRLNVESCGDYSLMQQKTTEL
                     LSFLERYVA"
     gene            complement(12531..13988)
                     /locus_tag="LAF92_003438"
     CDS             complement(12531..13988)
                     /locus_tag="LAF92_003438"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HBI9792390.1"
                     /translation="MLNVQEISTLFPVVMAGGSGSRLWPLSRALYPKQFLSLIGRTSM
                     LQKTLKRLDGMYCQKPVVICNELHRFVVAEQLRNIDMLSNNIILEPEGRNTAPAIALA
                     ALTAIKENTEQDPLLLVLAADHVIEDEVEFRNNVLSAIELANQGHLVTFGIVPTHAET
                     GYGYIKRGEVLDYGMPDETVAGYAVEAFVEKPDLATAEKFLASQEYYWNSGMFLFRAK
                     RYIEELKKYRPDILDACERAFNSSRPDLDFVRINSEEFLKCPAESIDYAVMEKTAEAA
                     VVPLSVGWSDVGSWASLWDISTKTKDGNVLQGDIIVSNSQNSYINAESALVAAVGIDN
                     LVVIQTKDAVLIANKNDVQDVKKIVTKLKLEGRQEYYSHSEVFRPWGKYETIGQGNRY
                     KVKRITVKPNEGLSLRLHHHRSEHWIVVSGTARVTLSGKSRLLVTNESVYIPAGTDYS
                     LENQGIIPLELIEVSSGDYLEEDDIVRFTNRYQNQ"
     gene            complement(14010..14510)
                     /locus_tag="LAF92_003439"
     CDS             complement(14010..14510)
                     /locus_tag="LAF92_003439"
                     /EC_number="3.2.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF011963.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBI9792391.1"
                     /translation="MVFKQSEHATRLTMFLPDEEFKKIIQGSPLISIDLLVRNEYNEY
                     LLGKRLNEPAKNYWFVPGGRIQKNETISQAIDRISVNELSIHLKLEQGRFHGVWQHFY
                     HNNFFNDEFTTHYIVLAYIFNAERESIHPPQTQHQRFRWLRKEEILIDSTVHKYSASY
                     FSDNDD"
     gene            complement(14474..15439)
                     /locus_tag="LAF92_003440"
     CDS             complement(14474..15439)
                     /locus_tag="LAF92_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBI9792392.1"
                     /translation="MNKTRVFVAGHKGMVGAAIVRQLTQRSDIELLLRDRSQLDLLDS
                     AAVNQFIGQEKPDQIYLAAAKVGGIIANNTYPAEFIYQNLMIEANIIHAAHCHGVQKL
                     LFLGSSCIYPKMASQPIQEDELLSGILESTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPNDNFHSENSHVIPGLMKRFHQAKMNNAPKVVVWGSGKVRREFLHVDDMA
                     AASIYVMEVERSVWQANTEEMLSHINVGTGVDCTIRDLANMIAKITGYKGRIEFDASK
                     PEGTPRKLLCVERLAKLGWRYSIDLQSGLQDTYTWFLNNQNMLRD"
     gene            complement(15445..16563)
                     /gene="gmd"
                     /locus_tag="LAF92_003441"
     CDS             complement(15445..16563)
                     /gene="gmd"
                     /locus_tag="LAF92_003441"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBI9792393.1"
                     /translation="MSKVALITGITGQDGSYLAELLLEKGYIVHGIKRRASSLNTERI
                     DHIYQDFCTANTNFHLHYGDLTDSLNITRIISEVQPDEIYNLGAMSHVAVSFESPEYT
                     ADVDAIGTLRILEAIRFLKLEKKTRFYQASTSELYGLVQETPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRALANISLGLEKCLYL
                     GNMDSLRDWGHAKDYVKMQWLMLQQEQPDDYVIATGIQYSVREFVRLAALELGIKLRF
                     EGTGVDEIGIVEDISGDKVVGVQVGDVIVSVDPRYFRPAEVDTLLGDAAKAREQLGWK
                     AETSLRELVSEMVSSDYNTAKQHAFLKNNGYSVNIPQE"
     gene            complement(16693..17568)
                     /gene="rfbA"
                     /locus_tag="LAF92_003442"
     CDS             complement(16693..17568)
                     /gene="rfbA"
                     /locus_tag="LAF92_003442"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBI9792394.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTTAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPRPDGLAQAFIIGEEFIGC
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNFAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFISKEQLQKLSESL
                     IKNNYGRYLQKIINE"
     gene            complement(17626..18525)
                     /gene="rfbD"
                     /locus_tag="LAF92_003443"
     CDS             complement(17626..18525)
                     /gene="rfbD"
                     /locus_tag="LAF92_003443"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBI9792395.1"
                     /translation="MNILLFGKTGQVGWELQRSLAPLGNLIALDVHSTEYCGDFSNPE
                     GVAETVRKIRPDVIVNAAAHTAVDKAESEPEFAQLLNAISVEAIAKAANEVGAWLIHY
                     STDYVFSGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCVKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHVIRVAQNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGISLALNKLNAMPTTAYPTPARRPLNSRLNTEKFQQNFE
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(18525..>19183)
                     /locus_tag="LAF92_003444"
     CDS             complement(18525..>19183)
                     /locus_tag="LAF92_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBI9792396.1"
                     /translation="DEVNSDEELPLFTETTAYAPSSPYSASKASSDHLVRAWKRTYGL
                     PTIITNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHARALYT
                     VVTEGKVGETYNIGGHNEKKNIDVVFTICDLLDEIMPKEKSYREQITYVADRPGHDRR
                     YAIDADKISRELGWKPQETFESGIRKTVEWYLANTKWVENVKSGAYKSWIEQNYEGRQ
                     "
BASE COUNT         6006 a         4246 c         3458 g         5473 t
ORIGIN      
        1 tttggccacc tgaacagagg tgatatgctc acctcagaac aacacaggtg ctccaatgaa
       61 aaaaagaaat ttcagcgcag agtttaaacg cgaatccgct caactggttg ttgaccagaa
      121 ctacacggtg gcagatgccg ccaaagctat ggatatcggc ctttccacaa tgacaagatg
      181 ggtcaaacaa ctgcgtgatg agcgtcaggg caaaacacca aaagcctctc cgataacacc
      241 agaacaaatc gaaatacgtg agctgaggaa aaagctacaa cgcattgaaa tggagaatga
      301 aatattaaaa aaggctaccg cgctcttgat gtcagactcc ctgaacagtt ctcgataatc
      361 gggaaactca gagcgcatta tcctgtggtc acactctgcc atgtgttcgg ggttcatcgc
      421 agcagctaca gatactggaa aaaccgtcct gaaaaaccag acggcagacg ggctgtatta
      481 cgcagtcagg tacttgagtt gcataacatc agccatggtt ctgccggggc aagaagcatc
      541 gccacaatgg caaccctgag aggcttcaga atggggcgct ggcttgccgg caggctcatg
      601 aaagaactgg gactggtcag ttgccagcag cctgcgcacc gttataaacg aggtggtcgt
      661 gaacatgtca ctatcccgaa tcaccttggg cggcagttcg cagtgacaga gccaaatcag
      721 gtatggtgcg gcgacgtgac gtacatctgg acggggaaac gttgggcata ccttgccgtt
      781 gttctcgacc tgtttgcaag gaaaccggta ggttgggcaa tgtcgttctc tccggacagc
      841 agactgacca tcaaagcgct gaaaatggcc tgggaaatcc gcagtaaacc agccggggta
      901 atgttccaca gcgatcaggg cagccactat acaagcaggc agttccggca gttactgtgg
      961 cgttaccaga tcaaacagag tctgagtcga cgaggaaatt gctgggataa cagcccgatg
     1021 gagcgcttct tcaggagtct gaaaaacgag tggataccgg tgacgggtta catgaacttc
     1081 agcgatgctg cccatgaaat aacggactat atcgttgggt attacaacgc gctcaggccg
     1141 cacgaatata acggtgggtt gccaccaaat gaatcggaaa accgatactg gaaaaactct
     1201 aaagcggtgg ccagtttttg ttgaccacta cattccgata ggcttctttt ttatctaacg
     1261 tcgcgttaaa ccgaatttca cctgactgca aaaatttttg aatctcttta tcaacgacag
     1321 gagatatccg atcattcagc attgcaacac gtgaaggcaa aatatctaat gcaacaacct
     1381 catggttttg tgcgattaga agcccattcg acaggcccac atagccggta ccggaaatgg
     1441 taattctcat ttcatgctct cagaattagc ttaactgtga atcatgatgt tttagcatcc
     1501 tgataagagt taaaattttt agcgctacgc tgctcccagc attaacgtaa caacaaaatc
     1561 agactttact tataagaata gctgatattt attaagttaa tattaagcaa tgaaatttaa
     1621 tctataggat tattggcaaa aaaaacccgg caatatatgc cgggcctcaa attattgctg
     1681 attaaattag attaatccag ccattcagta tggaacacac cttctttatc aatgcgctta
     1741 taagtatgcg caccgaaata gtcacgctga gcctggatca ggttcgcagg cagcaccgcg
     1801 gcacggtagc tatcgtaata agcaaccgcc gcagagaagg ttggaaccgg aataccgttt
     1861 tgcaccgcat aagcgacgac atcacgcagt gcctgttggt agtcgtcagc gattttctta
     1921 aagtacggag ccaacagcag gttagcgatt tttggatttt cggcatacgc atcggtgatt
     1981 ttctgcagga actgcgcacg gatgatgcag ccagcacgga aaatcttcgc gatttcaccg
     2041 tagttcagat cccagttgta ctcttcagac gcagcacgca gttgagagaa gccctgagcg
     2101 taagaaacga ttttgcccag atacagcgca cgacgaacct tctcgataaa ctctgctttg
     2161 tcgcctgctg gctgtgcttg aggaccagac agaactttag acgcagcaac acgctgctct
     2221 ttcagagaag agatataacg agcaaacaca gactcggtaa tcaacgacag cggctcgccg
     2281 agatccagcg cactctggct ggtccattta ccggtgcctt tattagctgc ttcatccagg
     2341 atcacatcaa ccaagtagtt accgccttca tcttttttgg tgaagatatc tttggtgatg
     2401 tcgatcaggt agctgctcag ttcatcgtta ttccactcgg taaaggtctg tgccagctct
     2461 tcgttggaga gattcagacc acctttcagc agtgaatagg cttcagcaat caactgcata
     2521 tcaccgtatt caataccgtt gtgaaccatc ttcacatagt gaccagcacc gtctgcacca
     2581 atataggtaa cgcacggttc gccatcttca gctacggcag caattttggt caggataggc
     2641 gcaaccaatt cataggcttc tttctgaccg ccaggcatga tagaaggacc tttcagtgcc
     2701 ccctcttcac caccggaaac tccagtacca ataaagttaa agccttctgc agaaagctca
     2761 cggttacgac gaatggtgtc ctggaagaag gtattaccgc catcaataat gatgtcgcct
     2821 ttttcaaggt atggcttgag ggaatcaata gcagcatccg tgcctgtgcc tgctttcacc
     2881 attaacagga tacgacgagg cgtttccaga gattcaacaa actctttcac cgtatagtaa
     2941 ggaaccagtt ttttgcctgg gttttcggca atcacttctt ccgtcttttc acgggaacgg
     3001 ttgaaaatag agacggtata accacggctt tcgatgttga gggcaaggtt gcgtcccatc
     3061 actgccatac cgacaacgcc gatctgttgc tttgacatta catactcctg tcagttatga
     3121 atactactaa cgttacactg tcaatgcagc tttaatgtta acccagaaca tctggcttgt
     3181 tgtaaaaaca accggaccaa aaatttcaag tgttaaaaga gaattttttt ggatttgttt
     3241 catgagccat agccacaagg atttttttgt tgccagttcc atgaatcacg catcattata
     3301 tctaagtcac ggctcgctga ccatttaagt tcttgctctg ccaaagatgg gtttgcccaa
     3361 catgcagcca catctccagg ccggcgatcg acaaatcgat gcggaatagt cttatggctc
     3421 tgtcgctcaa aagctgcgat caaatcaagt acggaatagc ccttgccggt gcctaggtta
     3481 taagttctgc aattgctgcc ttgtgcttta ttctgcaaag cggcaatatg gccttccgcc
     3541 aagtccatta catgaatata gtccctaaca ccagtaccat catgggtagg ataatcgtgt
     3601 ccgtagatca acaactctgg aagtttacca actgcaactt ggcaaatata aggtacgaga
     3661 ttgcagggga tgccattagg atcttctcca ataaaacctg acggatgagc gcctactgga
     3721 ttaaagtatc gcaagattgt aatatcaaac tgtggatatg attttgcaat gtcatttaga
     3781 atttgctccg tcatcaattt cgatgcgcca tagggattcg tcgttccacc aatttttgaa
     3841 ctctctttaa gaggtactgt ttcaggatta ccataaacag ttgctgagga actaaatatc
     3901 aactcatgca cgttatgagc catcatctcc tgaagtaaaa cgatactcgc agtaacgtta
     3961 ttattatagt actcgagtgg gaattgcacg gactcagaaa ccgatttcaa accagcaaag
     4021 tgcatgacct gttttatcga attttcagtg aaaatttttt gtaataaagc gcgatttgaa
     4081 acatcacctc tataataagt gatatcacgc tttgtcaaca atttaacacg gcacagtgct
     4141 tcataggagg aattgctatg attatccaga acaacgactt catagcctcg ctctaaaaga
     4201 gataatacag tatgtgaacc aatatatccc gcaccaccag taactaatac tgacatattt
     4261 actccttata aacgcagtga aactttattt acttgcctca ggataaaatc atagacacca
     4321 gcctatcgca cacttgttct tggtcaaatt tttcgagcgc aatctcacga ctggtacgcc
     4381 ccatagttaa gatcagatct ggttgctcta taaatacgaa cattttctcg gcaagcgcct
     4441 ctgcattcca aggtggtatc aagaaaccat tccgcccatc aatcaccgtt tcacgacatc
     4501 ccggaacatc agtagtaatg atagctctac ctacagccat cgcttcctga gtacttctgg
     4561 gcacaccttc acgatatgag ggtaaaacaa acacactact attaatgagg tgagatttga
     4621 tatcagaaac ttgtcccaga aattcaatac taccgttttt tacccatcct tcaaattctg
     4681 aactggtaat acctccaggg ttatcatcaa aggcaccggc gatactaaat tttacttcag
     4741 gataacgtag cttaactttc tcggcagcat gcacgaattc acgaataccc ttttcgacta
     4801 aaagtcgaga gatcatcgtg aaatgcaccg gcttaacggg tgctggggaa taattgtact
     4861 cactaagatc aactccaata cctccgataa tcttagtcga tttaacagaa atattattct
     4921 ggagcaatag ttctttgtga tcatcagggt ttaaaaaaat tacttttcga cacagcggta
     4981 gagccaaacg gtataaagtt gtttgtactg ttcggagaat ttttttctta ataccatctg
     5041 gagtggcatg cctagtatag cagtacccca atccttcaag cagaacatcg atttttgtaa
     5101 tgccaataat acgcgcagca agagtgccaa agatcaccgg ctttgggaaa aaacagaaaa
     5161 caatgtcagg ctcaattttt ttaattatcc ttgaaagctt aatagtgttg aacagatcag
     5221 ataagggatt tgtacctgag cgcgagaact cgtattttgc aggatgcgcc cccatattga
     5281 taatacgttt aaaattttcc gcatcattat ccatagttaa ggtatgcaca acacaatttt
     5341 ttgatacaag cttttctatt agccttttcc taaaaagaac aatagttcca ctctggtttc
     5401 ctataagcaa tatacgcatc tgcaaacccc aagattaatt ctcagttact gaaaagtaaa
     5461 ttgtgaattt taacactgct tctgataact aaactatatg taactgatcg tattttacat
     5521 gcattcgcac aaagctagag taagttgtca acagcaaagt caattcttac ctcgaataaa
     5581 aaacttcata accgaaatca aagaagctat aagaaaaaaa aagcttttat ttatcagcat
     5641 ctcacgaacc attaaccctg cctgctctgc ataagctaac ggctttcgct gcgccgtgct
     5701 actatgaaca cgatagctaa ataaaacatc agttatattg tcccatctga ctgacatccg
     5761 cgataatctt agccataaat cataatcttc tgatttgaaa ccagcgttgt agccgcacgc
     5821 atttataatt gtttctttct tgtacatcac tgagggatga caaaatgggt tagtaaatgg
     5881 aagtttttta ttaatattac ttccaactgg atagttcacc gtagcgatta ttctatctga
     5941 agcatctatt ttattaatag cgcaaccaat catatctaga ttcttggatt caatataatt
     6001 aagttgaacc tcaattctat tataatgaga aatatcatct gcatccattc tggctacata
     6061 tttataatta cagtgtaaca accccagatt caatgcaaaa gaaagttgtt ctaccccaca
     6121 tttaacaaac ctaacattag tcaaattacc ataccactct gataaagttt ctatgatgga
     6181 ttcgcaatga catccattaa caactattac taattcatct acagggcggg actgatttaa
     6241 gatactatca atcgctacgc gagtatagtt atcaatatta ttaagtgcta ataagccact
     6301 aactccaaat ttctcgttca tgatgtttaa tacttaaata tttattctga tatggatgta
     6361 gtgctacaat ttaatccagc catcgacgta taattgacca atactatcgt tcaaagtcca
     6421 tcgctcagga catatgacga tcttctctgg attagcattg agtacagccc cccaccaact
     6481 ataagtgcta ttagcaataa tattatgctt acagagcgac ataagataca tgtcaaccgc
     6541 cgaggaggtt gagctaggat tctcaacaaa ataggcatct gaaactccag aaaatgcctc
     6601 tttaacccaa gcaatatcat cagaaaaaaa tacaaattta gggtctttaa tcctagaagt
     6661 tatcaattcg attgattttt tataatactc aatatcacat attccgtgaa atgcactagc
     6721 attgacattc gttacataat ccccccggcg aatatgtaca gcaatcgtgt tatcatgctg
     6781 gctaatagtt cgataagaat ccagtctgcg catttcagca tctacttcag gagtaagtgt
     6841 aaattgctgg cgaatgatat taatattctc ctttaggaga ctataatttt ggaaataacc
     6901 aataacataa tcgttttctc gtgcattcat atgataagtg aaggcgtctt cattatagat
     6961 atgaattaaa cgcgtaattt tacggaatgc agaagagtta ctataccaag gaatacgagt
     7021 aagaattttt aatatcaaca cttttttacg tgtattactt ttttctttac aattactctt
     7081 tcttgtatac tgaacgttaa actgaaagat ctccagtttt ctgaaggtgt ctttactggg
     7141 ttgattatca taatagctat catcaacaac aatgcttcct ccaaatttaa cttcaagcac
     7201 tttcgctgca gcaaactgga atagttgatt tcccaaaccg cctgagaatc taataatcat
     7261 catcattcct tctctaaaat tgaataacta gcaccggtgt gctaacagaa atcgtttatt
     7321 ccatttctta ttaaaatctc gcaacgtcct tgcatgataa aattataatt aggaggctca
     7381 gttactccag gcaaatgaaa gcggtaatca ctcataagcg gtaaatatat tggggagaca
     7441 actattctga gaccaataac aaaaacaact attgaaatca atatccctcg agagatatac
     7501 ttggaaaaca atctctcaat atctggaata agataaggat agcaaaaata agtaaaacga
     7561 ttaaatacag gaaaaggtat ccctaataat acaacaccaa aacaaaacac ttgccatttt
     7621 ttattaacaa tattttcttt tctcttatac aaacagagta atgataaaat aaaatacagc
     7681 aagaagccaa gcgcaaatga tggagagaat atattggaat ttaaatagtt tgatattttc
     7741 tgagttatga aataaacaat ggtggaatta aaatcgaata ctagaaatgc caagctcacc
     7801 aatactacac aagaaaatat caacatcatt ttcctgcgtt tttcctcttt actactgatt
     7861 atgaaatata agccaattga gaaaatagca gagtagtgaa acagacaggc aactaacgca
     7921 tataaaataa attttctttt ttgagaagcg aatagaataa tagctaacgc aataccttgt
     7981 ctgacctgct cggcaaaaag cacaaatcca aacaatgcaa aaataaaaaa gaaaacaaat
     8041 ccagagtatc tgaacctaac gcaataacgg taaacaaaga aatatattat aacgttactg
     8101 agcatataaa cagaatgata ctcgaatccc atccaagcac aaactttcat caacgcagta
     8161 aaaccaggct caaatacatt gttgaacagt tcaatattgt ctatacgagt atagaaatca
     8221 taataattaa tccaatcata tgccggaaaa taccacccac tcataacagc aaatgttgca
     8281 aaaacattaa acgtagcaag atatttatta tttacaaatg aacaaaaaaa taagtaaatt
     8341 aaaagaataa tatacatata gaaggccagt agccgtctcc aaacaataaa aatgtcgggt
     8401 atggatttat ctcatagagc taatatctgc aatactttct ttgcgaagtt atgcagatga
     8461 aaacaccact agcaatgagc ataaaaatac aatacgataa gtttagtact atactttaag
     8521 attcaaaata acagcaaaca taactttcat tttttttata aaatttttaa ttttggacgg
     8581 tttaaagtgg atgcctacgg agcctgagtg gccataaata tctgcaatct cacgtccgat
     8641 aacctgcctg taaggcatat tatcaataac ttcaaagaag acatgctcta aatgacgccc
     8701 attgagatca tcaaccctag tatgaatgct ggaaaatttt tccagataaa aatctttgct
     8761 aataatatag aatcttgtat caaccttatc aggagaaaag aaaggggcgg tgagaaaagc
     8821 ggcgcgacca gagaatactt ttatgcattc aaaataattg gataaccata attttgaagt
     8881 gcattttgca aatgcatcat actttttcaa aatttcacta ttagcaagtg caaaattaac
     8941 tatttggcct tcgccataac ctttgccaaa acgtttaaca ccttcgctat cattatgaaa
     9001 atttagaaat tctatatttt ggaatatatt ttcaagtaat tctctttcgt ccgaaaaatc
     9061 atagccgcta ccatcacaaa ttacaatagg aacttctcgg caatattcag ataacataaa
     9121 caaagcattc atagttgatt gcaatcgttt attactatca ctcaataccg taagcggtgc
     9181 atttgaaacg acacaggaag taatcaaaag cggaatcatt ttatttttgt tcattctgta
     9241 taaaccatct aaaaataagt aacaatgcca tatctacaat aactctaaat gtcgaagcta
     9301 cagcgacacc aattactccg taatatttaa tagcaaaata gaggaagcaa aaataaggca
     9361 cgagttctat aatatgaata atagcggtcg ctttaaagta ccctttcgct tggatttcag
     9421 cgaatgttac ttgtgcaaca gagttaacaa acatgccaaa caacaaaatg agtaaaatgg
     9481 taactgaatt cgttccatat tctgtaccga tccacatgtc caaaataaaa ctaggaaaca
     9541 ttagaaatgg tagaatgatt aaaaacgcac tactaccaac taaaagatat ccatggcgaa
     9601 gctgccttgt agcttccccc ttgtttaatg agtgactaaa taacgggaat agtactcttg
     9661 atatcgcatt agggaagata gagattctat tcactatttc tgaaggcgca gtataaaacg
     9721 ccacgttttt agcgcctaac atattagaaa gtataaaacg atcacaatag agaatcaatg
     9781 gacttataat attactgaca gaaacccaac caccatatga taacaagcac ttacaaatcg
     9841 ttttatcaaa aaaaaagaaa tttatttttg cataccgacg aatataataa tggtttatcg
     9901 caaaagaaat gatacgagcc agtgtcagac caagcacccc tgacatgagt gaagcatcac
     9961 aggcaataaa agccacagga gaaattgcca gaatcgagcc agtgactgtt ttctgcaggt
    10021 taatagactt aaagttttcc aagccttcca gataaccagt atatatctgc gaaaataata
    10081 gaaatggaaa tgagatacta ataattctca atgagcgcac tacatctgta aaatgttgtg
    10141 cagaaacatt aaatacccat gctattttgg ttgcatttag tgtaatcaga agcgccccca
    10201 atatactgat gacaagtata ataacaagcg atgtagaaag gatcacattc agccgatgtg
    10261 tatcctctct gtgcatagat acctcacgaa taactgctcg acttaagcct gcatcgagaa
    10321 tactcgcaaa cccaaaaatc gtaaagaaaa ttgtaaaaag accaaacagt tcgacaccta
    10381 atagacgagc aacgattccc atcgcaggta ttgcaatcag cgatggtata ataaagccgc
    10441 caatattcca aaaactattc tttaaaatat tcaattttgg tttacttagt aatcaaattt
    10501 tggagcatca acaaataata atcccaaatt atctttctca gaaagaagtg gctttccttc
    10561 taatggccac ttaattgcaa gttggggatc attccataat aaagtctgtt ctgccatcgg
    10621 attatagaag tctgtacatt tataaacaaa ctctgcgacc ttgctgataa cataaaaacc
    10681 atgtgccagt cctccaggaa tccacaactg tcttttgttt tcaccagaaa ggtagaaccc
    10741 ggcccacttg ccaaaagtac ttgagttttt tcgcaaatcc acaacgacat cataaacaac
    10801 accggaaaca acacgcacca acttaccctg agtgttgaca agctgataat gcaatcctcg
    10861 tagcgtacct ttacatgatt tagagtgatt atcttgaaca aattgcgttt tacctattgt
    10921 ctgctcgaac tttttctcat tccatgtctc cataaaaaaa ccacgagaat cactaaaaat
    10981 attcggctca atcagtttaa cgtcatttat agttgtatca ataacattca tattttttaa
    11041 catctcatca attgatatgc tggatggcct accacctcac aaatacctac cctacagcct
    11101 agaagcacat tcaattttat gccacatagc gctcgaggaa gctcagaagt tctgtcgtct
    11161 tttgttgcat tagcgagtaa tcaccgcacg actctacatt tagacgtaca agactttctg
    11221 tatttgatac ccgaatattg aacctccatt gcgcaaattc catgctgaca ccatccgtgt
    11281 aatccacatg ctgtgcagaa ccagaataac aatcacagag atcttttatg caacgttgtg
    11341 catcttcgat attgcgattg atttcaccgg aagcaggcca tgcggcgata gactcatcga
    11401 tcaactggtt taacccaact ttctcaatgc tcagcatttc aataactaat aaccacggaa
    11461 tcataccact atcacagtaa aaaaaatcgc gaaaataatg atgggcactc atttctccac
    11521 catagattgc gttttcagca cgcatacgat ctttaataaa tgcatgccct gttttagaaa
    11581 gtattggtat accaccggct ttcctaacga tatctattgt attaaatagg agtctcggat
    11641 catgaataat ctttgcgcct ggctcctttt tcaaaaaaga tgctgcaagg aggccaacaa
    11701 tataatagcc ctcaataaaa cgaccattgc tatcaaaaaa gaaacaacgg tcaaaatcac
    11761 catcaaacgc aatgccaaga tcggctccgt gctcaactac tgcatcgtgg gtatcctgtc
    11821 gacactcagg gagcagcggg ttgggaattc cgtgtggaaa agagccatcc ggttcattat
    11881 taactttgat aatagttatt ggaatggatt gttcttgaaa tcgtttttcc aagcaatcta
    11941 tgaccggacc tgcagctcca ttaccagaat taataactaa ttttaatggt ctgagattac
    12001 taatatttat gaaagatagt atatggtcaa tgtactcctg cttcagtgat attcttttat
    12061 acttcccctt aaattcattt tttgattcgt caatcttcaa cgtctcagca agcaatttaa
    12121 tttcataaag accgctttcg cagctgattg gtttagaatc tcgcccgacg attttcatac
    12181 cattataatc aatagggtta tggctggctg taacctctat accaccatca agtttaagat
    12241 atgaggttgc gaaataaatc tcttctgtac caaccattcc aatatcaata acgtcaacgc
    12301 cagcttcccg taacccatta gctaaggcta tttttaaaga ctcactcgtt aaacggcagt
    12361 ctcctcctac aacaacagag cgcgcttttg tatacaaacc aaatgctttt ccaattttcc
    12421 aagctatttc tgtcgtaata tcgtgattaa tacgacctcg tatatcatat gttttaaagc
    12481 aagataactt cgacattttt ctatattctt aatataagag aataatctta ttactggttt
    12541 tggtagcggt tagtgaaacg aacgatatcg tcttcttcga gatagtcacc agaacttact
    12601 tcaataagtt ctaatgggat aatgccctgg ttttcaaggc tataatccgt ccccgctgga
    12661 atataaacag attcattagt tacaagtaat ctgcttttac cagataatgt gacacgggcg
    12721 gtccccgaga ctacgatcca atgctccgaa cgatgatgat gtaatcttaa cgataatccc
    12781 tcgtttggct taactgtaat acgtttcact ttgtaacgat taccctgacc gattgtctca
    12841 tatttacccc aaggccgaaa aacttcgcta tgactatagt attcttgtct accttcgagt
    12901 ttcaatttgg tgacaatttt cttaacgtcc tgaacatcat ttttattagc aattagaaca
    12961 gcatcttttg tctgtataac aaccagatta tctatcccta ctgctgccac taaagcagat
    13021 tcagcattga tgtaactatt ctgtgaattg ctaacaataa tatcaccctg taatacgtta
    13081 ccgtctttgg ttttcgtgga aatatcccat agagacgccc acgaaccaac atcgctccag
    13141 cccacagaca gaggtacaac agcagcttca gctgtttttt ccattactgc gtagtcaatc
    13201 gattcggctg gacatttcag gaactcttct gagttgattc tgacaaaatc taaatcaggt
    13261 ctcgatgagt taaatgcccg ctcgcatgca tcgagaatat ctggacgata ttttttcaac
    13321 tcttcaatat atcgtttcgc tcgaaacagg aacatcccac tattccagta atactcttgc
    13381 gaggctaaga atttttctgc cgttgccaaa tcaggttttt caacgaaagc ttcaactgca
    13441 taaccagcta cagtttcatc cggcatgcca tagtctaaga cttcgcctct tttaatatag
    13501 ccgtaacctg tctctgcatg tgtcggaaca atgccaaagg tgactaaatg cccctgattt
    13561 gccaactcga tagcagagag aacattattt ctaaactcaa cttcatcttc gattacgtga
    13621 tccgcggcaa gaactaaaag caaaggatct tgctcagtat tttcttttat tgctgtgaga
    13681 gctgctagtg caatcgcagg agccgtattt ctcccttctg gctcgagaat aatattatta
    13741 gacaacatgt caatattacg caattgctct gcaacaacaa aacgatgcaa ttcattacat
    13801 attacaaccg gtttttgaca atacatccca tcgagccgct ttaaggtctt ttgcagcata
    13861 gaggtccgac caattaacga caaaaattgt tttggataca atgctcttga taaaggccac
    13921 aatctactac ctgaaccacc agccataacc acggggaaaa gcgtagatat ttcttgtacg
    13981 tttagcatat atattaccta atttattttc taatcatcgt tatctgaaaa ataggatgct
    14041 gaatatttat ggaccgttga atctattaaa atttcctctt tacgtaacca tctaaagcgc
    14101 tgatgttgcg tctgaggggg atggattgat tctctttcag cattaaaaat atacgctaaa
    14161 acaatataat gagtggtaaa ttcatcatta aaaaaattgt tgtgataaaa atgttgccac
    14221 acaccatgaa atctaccttg ttccagtttt aaatgaatgc tcaattcatt tacacttatt
    14281 ctatctatag cttgcgaaat cgtttcattt ttctgtatgc gaccaccggg tacaaaccaa
    14341 tagtttttcg caggttcgtt aagcctttta cccaaaaggt attcattata ctcatttctg
    14401 accagcaaat cgatagaaat taatggactc ccttgtataa tttttttaaa ctcttcgtcc
    14461 ggcaaaaaca tcgttaatct cgtagcatgt tctgattgtt taaaaaccaa gtataggtat
    14521 cctgcaaacc actctgcaga tcaatactat atctccatcc cagttttgct aagcgctcca
    14581 cgcacagcaa ttttctcggc gttccctccg gtttcgatgc atcgaactca attcgacctt
    14641 tataccccgt tatttttgca atcatgttag ccaaatcgcg aatggtacag tcaacgccgg
    14701 taccaacatt gatatgcgat aacatctctt ctgtgttcgc ttgccataca ctacgctcca
    14761 cctccatgac ataaatactg gcagcagcca tatcatcgac atgcaaaaac tcacgcctca
    14821 ccttgccact tccccacacc acgactttag gagcattgtt catttttgcc tgatgaaaac
    14881 gtttcattaa gccaggaata acgtgtgagt tttcagagtg gaagttatca ttcggtccgt
    14941 ataggttggt cggcattaca gagcgataat cacgaccata ctgtctatta taagactcgc
    15001 aaagcttaat gccggcaatt tttgcaattg catagggttc attcgtagat tctaatatcc
    15061 cactcaataa ctcatcttcc tgaataggtt gagaggccat tttagggtaa atacatgaag
    15121 atccgaggaa taacagtttc tgaacgccat ggcaatgagc agcatggata atatttgctt
    15181 caatcatcag attttgatag ataaactcgg ccggataggt attattcgca ataattcccc
    15241 caacttttgc ggcagcgaga taaatctgat ccggcttttc ttgcccgata aactgattga
    15301 ctgcagctga gtcaagcaag tctagttggc ttctatccct taaaagtaat tctatatcac
    15361 tcctttgggt caactgccgt acgatggccg cccccaccat tcctttatga cctgcgacaa
    15421 aaacacgggt tttattcata gtattcattc ctgcggaata ttaacagagt aaccattgtt
    15481 cttaagaaaa gcgtgttgtt tcgcagtatt gtaatcgctt gataccatct cagaaaccag
    15541 ttctcgcagc gaagtctcag ctttccagcc caactgttca cgtgcttttg ccgcgtcacc
    15601 taacagggta tcaacctctg cagggcgaaa ataacgcgga tcaacagata caatcacatc
    15661 accaacctgc acacctacaa ccttatcccc agaaatatct tcaacgatac caatctcatc
    15721 aacaccggtt ccttcgaaac gtagtttgat gcctagttca agagcagcca aacgcacaaa
    15781 ctcgcgtaca gaatattgaa tcccagtagc aatcacgtag tcgtcaggtt gctcttgctg
    15841 caacataagc cactgcattt ttacataatc ttttgcatga ccccaatccc gaagagaatc
    15901 catattcccc agataaagac atttttccag tcctaatgaa atattcgcca atgctctagt
    15961 aattttacgt gttacaaacg tttcaccacg gcgtggggat tcatggttaa acaaaatacc
    16021 attacatgca taaattccat atgattcacg ataatttacg gtgatccaat aagcatacag
    16081 cttcgcaacc gcatatggcg aacgcggata aaagggagta gtttcttttt gtggggtctc
    16141 ctgaaccaat ccatacagtt ctgaagttga agcttgatag aaccgtgttt ttttctctag
    16201 ttttaaaaaa cgaattgcct ctaatatacg cagtgtaccg atggcatcta catcggcagt
    16261 atattctggc gattcgaaag agacggcaac atgactcatt gcacctaagt tatatatctc
    16321 atcgggttga acttcagaaa taatacgcgt aatgttcaag ctatcggtta aatcaccgta
    16381 atgcaaatgg aaattagtgt ttgcagtgca aaagtcttga taaatgtgat caatgcgttc
    16441 agtattaaga gacgatgctc gacgcttaat tccatggaca atataccctt tctcaagcaa
    16501 taattcggcc aaataggaac catcctgtcc ggtaatacca gtaatgagtg ccactttact
    16561 catgcttcac cttatttaaa atcgtattat tggctatatc aaaatatcga tacaagctgt
    16621 gcataaaacc tatttataaa tataaaatac gatattattc acaacccaca agacgtatta
    16681 atgaaatgct ttctattcgt taataatttt ttgcaaatac ctcccatagt tgttcttaat
    16741 gagcgactca gataattttt gtaattgttc tttactgata aatcctttac gataagcgat
    16801 ctcttcggga caagaaactt ttaatccctg acgttcttca atcgtcgcaa taaaattact
    16861 ggcctctatc agactttggt gagtacctgt atccagccac gcatagccac gtcccatcat
    16921 agcaacagac aatctaccct gttccatata gatacggtta atatcggtaa tttccagttc
    16981 gccgcgggca gagggtttaa gattttttgc catttctaca acgtcgttat cataaaaata
    17041 aagcccagtt acagcaaagt tgctttttgg ttgtaatggt ttttcttcca ggctaattgc
    17101 tgtgccgttt ttatcaaact caacgacacc atagcgctca ggatcattta cgtgataagc
    17161 aaataccgtt gcgccacttt ctttattaac agctgcatcc attaacttcg gcaggtcatg
    17221 gccgtagaaa atgttatcac ccaaaaccaa agcacaatca tcacagccaa taaactcttc
    17281 accgatgata aatgcctgcg caaggccatc tggcctcggt tgcactttgt actgaagatt
    17341 caggccccac tgacttccat cacccagcag ttgttgaaaa cgaggagtat cctgtggcgt
    17401 actgataatc agaatatcgc gaatacccgc caacattagt gtagaaagcg ggtaataaat
    17461 catcggttta tcataaatcg gtaacagttg cttactgaca gccgtagtca caggataaag
    17521 acgagttcca gaaccacccg ctaaaataat acccttacgc gttttcattt catcattcct
    17581 tttaattcat cttgctccac catcatgagc aagatgcaaa aactattaaa ttgctgttgt
    17641 cgtaaatagt tcattgagca ttcgtttcac ccccacctgc caatctggca agaccagttc
    17701 aaagttctgc tgaaactttt ctgtattaag gcgagaattg agaggacgac gagctggtgt
    17761 gggataagct gttgttggca tggcattgag tttgttaaga gcaaggctaa tcccggcttt
    17821 acgcgcctct tcgaaaacca acgcggcata gtcatgccag gttgtggtgc cgctggcaac
    17881 cagatggtac aaaccagcaa cttcgggctt gttctgcgct acgcgaatga cgtgtgcagt
    17941 gcagtcagcc agcagctcag cacctgttgg tgcaccaaac tgatcgttaa taaccgctaa
    18001 ttcttcacgc tcttttgcca gacgtaacat cgttttggcg aagttatttc ctttacctgc
    18061 ataaacccaa ctggtacgga aaataaggtg cttcacgcaa tgttcctgta atgccttttc
    18121 tccggctaat ttggtttcac cgtaaacatt cagaggcgct gttgcatccg tttccagcca
    18181 tggcgtatcg ccgtttccag agaaaacgta atcagtggag taatgaataa gccaggctcc
    18241 aacttcattt gccgcctttg caatcgcttc aacacttata gcgttaagca attgtgcaaa
    18301 ctctggttct gactctgctt tatctactgc ggtatgagct gctgcattaa caataacatc
    18361 agggcgaatt tttctgacgg tttcggccac accttcagga ttactaaaat caccgcaata
    18421 ctctgtggag tgaacatcaa gcgcaatcaa gttccccaga ggagccagag agcgctgtag
    18481 ctcccagcct acctgccctg ttttaccaaa aagaagaata ttcattactg gcggccctca
    18541 tagttctgtt caatccacga cttataggca ccacttttca cattttccac ccactttgta
    18601 ttggccaggt accattccac cgttttacga atcccgcttt caaatgtttc ctgcggtttc
    18661 cagcctaatt cacggctaat tttatcagca tcaatagcat aacgacggtc atgccccgga
    18721 cgatcggcta cataggtaat ttgctcacga taagattttt ctttcggcat aatttcatcc
    18781 agcaaatcac aaattgtgaa aactacatcg atgtttttct tttcgttatg accaccgatg
    18841 ttataggttt caccgacttt accttcggta acgacggtat atagcgcacg tgcatggtct
    18901 tccacataca gccagtcacg aatttgatct cctttgccat aaatgggtaa tgctttaccc
    18961 tccagggcat tgagaatgac cagcggaatt aatttttccg ggaaatggta aggaccatag
    19021 ttattcgagc aattggtaat aatggtcgga agaccataag tacgtttcca tgcacgaact
    19081 aaatgatcgc tggatgcttt tgatgcagaa taaggactgc ttggcgcata agctgtcgtc
    19141 tcagtaaata gcggtaattc ttcatcacta tttacttcat cag
//
LOCUS       DAEVZA010000097        19070 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEVZA010000097 DAEVZA010000000
VERSION     DAEVZA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.097"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1107
                     /locus_tag="LAF92_003445"
     CDS             <1..1107
                     /locus_tag="LAF92_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBI9792397.1"
                     /translation="DNISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMR
                     KYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLV
                     TCQLDEQQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSER
                     RILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEI
                     KNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGIFATKNVLAFSRG
                     DSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPG
                     KVFTLRGGSLNINIPGRSALLLGKTGEPPNYLYL"
     gene            1443..2420
                     /gene="csiD"
                     /locus_tag="LAF92_003446"
     CDS             1443..2420
                     /gene="csiD"
                     /locus_tag="LAF92_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBI9792398.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2440..3708
                     /gene="lhgO"
                     /locus_tag="LAF92_003447"
     CDS             2440..3708
                     /gene="lhgO"
                     /locus_tag="LAF92_003447"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBI9792399.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            3731..5179
                     /gene="gabD"
                     /locus_tag="LAF92_003448"
     CDS             3731..5179
                     /gene="gabD"
                     /locus_tag="LAF92_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBI9792400.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5193..6473
                     /gene="gabT"
                     /locus_tag="LAF92_003449"
     CDS             5193..6473
                     /gene="gabT"
                     /locus_tag="LAF92_003449"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBI9792401.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            6711..8111
                     /gene="gabP"
                     /locus_tag="LAF92_003450"
     CDS             6711..8111
                     /gene="gabP"
                     /locus_tag="LAF92_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBI9792402.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8132..8794
                     /gene="csiR"
                     /locus_tag="LAF92_003451"
     CDS             8132..8794
                     /gene="csiR"
                     /locus_tag="LAF92_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBI9792403.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8795..9244)
                     /gene="kbp"
                     /locus_tag="LAF92_003452"
     CDS             complement(8795..9244)
                     /gene="kbp"
                     /locus_tag="LAF92_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBI9792404.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9328..9486)
                     /locus_tag="LAF92_003453"
     CDS             complement(9328..9486)
                     /locus_tag="LAF92_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBI9792405.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            9669..9968
                     /locus_tag="LAF92_003454"
     CDS             9669..9968
                     /locus_tag="LAF92_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9792406.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9978..10502
                     /gene="ygaP"
                     /locus_tag="LAF92_003455"
     CDS             9978..10502
                     /gene="ygaP"
                     /locus_tag="LAF92_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBI9792407.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(10549..10953)
                     /gene="stpA"
                     /locus_tag="LAF92_003456"
     CDS             complement(10549..10953)
                     /gene="stpA"
                     /locus_tag="LAF92_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBI9792408.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            11621..12070
                     /gene="alaE"
                     /locus_tag="LAF92_003457"
     CDS             11621..12070
                     /gene="alaE"
                     /locus_tag="LAF92_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBI9792409.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(12107..12451)
                     /locus_tag="LAF92_003458"
     CDS             complement(12107..12451)
                     /locus_tag="LAF92_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBI9792410.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            12603..12932
                     /locus_tag="LAF92_003459"
     CDS             12603..12932
                     /locus_tag="LAF92_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBI9792411.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            13180..13425
                     /gene="nrdH"
                     /locus_tag="LAF92_003460"
     CDS             13180..13425
                     /gene="nrdH"
                     /locus_tag="LAF92_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBI9792412.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            13422..13832
                     /gene="nrdI"
                     /locus_tag="LAF92_003461"
     CDS             13422..13832
                     /gene="nrdI"
                     /locus_tag="LAF92_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBI9792413.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            13805..15949
                     /gene="nrdE"
                     /locus_tag="LAF92_003462"
     CDS             13805..15949
                     /gene="nrdE"
                     /locus_tag="LAF92_003462"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBI9792414.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASSEYFSQYLQGNWQPKTTKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            15959..16918
                     /gene="nrdF"
                     /locus_tag="LAF92_003463"
     CDS             15959..16918
                     /gene="nrdF"
                     /locus_tag="LAF92_003463"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBI9792415.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            17273..18475
                     /gene="proV"
                     /locus_tag="LAF92_003464"
     CDS             17273..18475
                     /gene="proV"
                     /locus_tag="LAF92_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBI9792416.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            18468..>19070
                     /locus_tag="LAF92_003465"
     CDS             18468..>19070
                     /locus_tag="LAF92_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="with ProVX is involved in the high-affinity uptake
                     of glycine betaine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/betaine ABC transporter permease ProW"
                     /protein_id="HBI9792417.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVM"
BASE COUNT         4692 a         4729 c         4899 g         4750 t
ORIGIN      
        1 gataacatct ctaaggatga atgggaaaac ctccatgcat taaaagaaaa gcgtttaaat
       61 ggaatgccta aaacaacacc caaaagtgac caggttatta tgttgcaaaa tcaatacgtt
      121 cgtgaaatgc gaaaatatgg cgtacgaggt ttacgttatg atgcggcaaa acactcaaaa
      181 catgaacaaa tagaaagatc aataacccca ccgcttaaaa attataatga gcggttacac
      241 aatactaact tatttaaccc aaaatatcat aaaaaagccg ttatgaatta catggaatat
      301 ctggtaactt gtcagttgga tgaacaacaa atgtcatcac tgctttatga aagagatgat
      361 ttaagcgcca ttgatttttc attgctcatg aaaacgataa aagccttttc atttggtgga
      421 gatctccaaa cccttgcatc aaaaccgggt tccacaatct caagtatccc atcagaaaga
      481 cggatattga ttaacattaa ccacgatttt cctaacaatg gtaatctttt caatgacttt
      541 ctatttaacc atcaacaaga tgaacaatta gcaatggcat atatggccgc tctgccgttc
      601 agcaggcctt tagtttactg ggatggccaa gtattaaaat caacgactga aattaaaaat
      661 tatgatgggt ccacgcgtgt tggcggtgag gcgtggctta ataaaggttg ctctacctat
      721 cagcagctct acaatgaatt ccacgcatta tatatagata aagcaggaat atggagcgca
      781 tttgagggta tatttgcaac taaaaacgtt ctggccttta gtcgtgggga ttctgtgaac
      841 attaatcact ctcctcatga tggactagtt ataataaata aaggaaacga agaagttgaa
      901 ggtacctggc ctaacaaatt gcaacctgga atatataaaa acatggggag taatagcgtt
      961 aacattatta ttaataatac ccgaaaaatt atcccccctg gtaaagtatt tacgcttaga
     1021 ggcggatctc taaatatcaa tattcctggg cgtagcgctc ttcttttagg gaaaacagga
     1081 gaaccgccga actatctcta tttataattt aatgttatat ctgccccgat aaaacggggc
     1141 agataatatg tttagtttac taacggtcat tttgcagtga agccatttac tgttttttat
     1201 cgaccagata atctgttctc taatgttaac tccccctaac ctgttgcttt agttattcat
     1261 ttcctgtctc actttgcctt aataccctac gttaaatgtt actaatttgt tgcttttgat
     1321 cacaataaga aaacaatatg tcgcttttgt gcgcattttt cagaaatgta gatattttta
     1381 gattatggct acgaaatgag catcgccatg tcaccctaca tctcataaga ggatcgcttc
     1441 tgatgaatgc actgaccgcc gtacacaata acgctgtcga ttcaggccag gactatagcg
     1501 gattcactct catcccgtcg gcgcaatccc cgcgtctgct ggaactcacc ttcaccgaac
     1561 agacgaccaa acagtttctc gaacaggttg ccgaatggcc cgtgcaggcg ctggagtaca
     1621 aatcgtttct gcgttttcgg gtaggcaaaa ttcttgacga tctgtgtgcg aatcagctgc
     1681 aaccattgct gttgaagacc ctgctaaacc gtgctgaagg tgcgctgttg atcaatgcgg
     1741 tgggtatcga tgatgtggcg caggcagatg agatggtgaa gctggcaacg gcggtagcgc
     1801 atctgattgg tcgctcaaat ttcgatgcga tgagcggtca gtattacgcg cgtttcgtgg
     1861 tgaaaaatgt cgataactca gacagctatc tgcgtcagcc gcaccgcgtg atggaactgc
     1921 acaacgacgg cacgtatgtc gaagagatca ccgattacgt gctgatgatg aaaatcgatg
     1981 agcaaaacat gcagggtgga aattcgttgc tgctgcatct cgatgactgg gaacatctgg
     2041 accactattt ccgccatcca ctggcgcgtc gcccaatgcg ctttgccgcg ccgccgagca
     2101 aaaacgtcag caaagatgtt ttccatccag tgttcgacgt tgatcaacag ggtcgcccgg
     2161 tgatgcgcta tatcgaccag ttcgtccagc cgaaagactt cgaagaaggc gtgtggttga
     2221 gcgagctttc cgacgccatt gaaaccagca aaggcattct ttctgtaccc gttcccgttg
     2281 gcaaattcct gttgataaac aacctgttct ggctgcacgg tcgcgaccgc tttactccgc
     2341 acccggatct gcgccgtgaa ctgatgcgtc agcgcggcta cttcgcttac gccactcacc
     2401 actaccagac gcatcagtaa gcgcaaagga attgagcgga tgtatgattt tgtgattatt
     2461 ggcggcggca tcatcggcat gtcgaccgcc atgcaactga ttgatgtcta tcccgatgcc
     2521 cgcattgcgt tgctggaaaa agagtccggc ccggcctgtc accagacggg ccacaacagc
     2581 ggcgtgatcc atgccggggt ctattacacg cccggcagcc tgaaggcgca gttttgcctg
     2641 gcgggaaacc gcgccaccaa agccttttgc gatcaaaacg acattcgcta tgacaactgc
     2701 ggcaagatgc tggtcgccac gtccgatctc gaaatggaac ggatgcgcgc gttgtgggaa
     2761 cgcacggcgg cgaacggtat cgagcgcgag tggttaaacg ccgaggaact gcgcgagcgc
     2821 gaaccgaata tcaccgggct cggcggcatt tttgtgccgt ccagcggcat tgtcagctac
     2881 cgcgaagtaa cggcggcgat ggcaaaaatt ttccaggcca gaggcggcga gattatctat
     2941 aacgccgaag tcagcggcct tagtgagcat aaaagcggcg tggtgatacg tacccgtcag
     3001 ggcggcgact atgaagcatc gacgctgatt agctgttccg ggctgatggc tgaccggctg
     3061 gtgaaaatgc tcggcctcga accgggcttt atcatctgcc cgttccgcgg cgagtatttc
     3121 cgcctggcgc cggagcataa ccagattgtt aaccatctga tttaccccat tcccgacccg
     3181 gcaatgccgt ttttgggcgt gcatctcacc cgaatgatcg atggcagcgt caccgtcggg
     3241 ccaaacgcgg tgctggcttt caaacgcgaa ggctatcgca agcgcgactt ctcatttagc
     3301 gacacgctgg agattttggg ctcgtcgggg attcgccggg tgctgcaaaa ccatctacgc
     3361 tcaggactgg gcgagatgaa aaactcgctg tgcaaaagcg gctatctgcg gctggtgcaa
     3421 aagtattgtc cccggctttc gttaagcgat ctccagccct ggcccgccgg tgtgcgggcg
     3481 caggcggtat cgccggacgg caagctgatt gacgattttc tgtttgtcac caccccgcgc
     3541 acgatccaca cctgcaatgc gccctccccg gcagcgacat cagcaattcc tattggtgcg
     3601 catattgtca gcaaggtaca aacgctgttg gcaagccaga gtaaccccgg acgcacgctg
     3661 cgagcggcac gtagtgtgga tgccttacac gccgcattta atcaataacc tttgaaaaca
     3721 ggacgtaaca atgaaactta acgacagtaa cttattccgc cagcaggcgt tgattaacgg
     3781 ggaatggctg gacgccaaca atggtgaagt catcgacgtc accaatccgg cgaacggcga
     3841 caagctgggt agcgtgccga aaatgggcgc tgatgaaacc cgcgccgcta tcgacgccgc
     3901 caaccgcgcc ctgcccgcct ggcgtgcgct caccgccaaa gaacgcgcca acattctgcg
     3961 caactggttc aatttgatga tggagcatca ggacgattta gcgcgcctga tgaccctcga
     4021 acagggcaaa ccactggccg aagcgaaagg tgaaatcagc tacgccgcct cttttattga
     4081 gtggtttgcc gaagaaggca aacgcattta tggcgacacc attcccggtc atcaggccga
     4141 taagcgcctg attgttatca agcagccgat tggcgttacc gcagctatca cgccgtggaa
     4201 cttcccggcg gcgatgatta cccgcaaagc cggtccggcg ctggcggcag gctgcaccat
     4261 ggtgctgaaa cccgccagtc agacgccgtt ctctgcgctg gcgctggcgg agctggcgat
     4321 tcgcgcgggc attccggctg gggtatttaa cgtggtcacc ggttcggcgg gcgcagtcgg
     4381 taacgaactg accagcaacc cgctggtgcg caaactgtcg tttaccggtt cgaccgaaat
     4441 tggccgccag ttaatggaac agtgcgcaaa agacatcaaa aaagtgtcgc tggagctggg
     4501 cggcaacgcg ccgtttatcg tctttgacga tgccgatctc gataaagccg tggaaggtgc
     4561 gctgtcctcg aaattccgca acgccgggca aacctgcgtc tgcgccaacc gtttatacgt
     4621 gcaggacggc gtgtatgacc gctttgccga aaaattacag caggcggtga gcaaactgca
     4681 catcggcgac gggctggaga atggcgtcac catcgggccg ctgatcgatg aaaaagcggt
     4741 agcaaaagtg gaagagcata ttgccgatgc gctggagaaa ggtgcgcgtg tggtttgcgg
     4801 cggtaaagcg gatgaacgcg gcggcaactt cttccagccg accattctgg tggacgttcc
     4861 agccaacgcc aaagtgtcga aagaagagac gttcggcccc ctcgccccac tgttccgttt
     4921 taaagatgaa gccgatgtga ttgcgcaagc caatgacacc gagtttggcc ttgccgccta
     4981 tttctacgcc cgtgatttaa gccgcgtctt ccgcgtgggc gaagcgctgg agtacggcat
     5041 cgtcggcatc aataccggca tcatttccaa tgaagtggcc ccgttcggcg gcattaaagc
     5101 ctcgggtctg ggtcgtgaag gttcgaagta tggcatcgaa gattacttag aaatcaaata
     5161 tatgtgcatc ggtctttaac tggagaacgc gaatgagcag caataaagag ttaatgcagc
     5221 gccgcagtca ggcgattccc cgtggtgttg ggcaaattca cccgattttc gctgaccgcg
     5281 cggaaaactg ccgggtgtgg gacgttgaag gccgtgagta tcttgatttc gcgggcggga
     5341 ttgcggtgct caataccggg cacttgcatc cgaaagtggt ggctgcggtg gaagcgcagt
     5401 tgaaaaaact gtcgcacacc tgcttccagg tgctggccta cgagccgtat ctggagctgt
     5461 gcgagattat gaatcagaag gtgccaggca attttgccaa gaaaacgctg ctggtcacca
     5521 caggttctga agcggtggaa aacgcggtga aaatcgcccg cgccgccacc aaacgtagcg
     5581 gcaccatcgc ttttagcggc gcgtatcacg ggcgcacgca ttacacgctg gcgctgaccg
     5641 gcaaggtgaa tccgtactct gcgggcatgg ggctgatgcc gggtcatgtt tatcgcgcgc
     5701 tttatccttg cccgctgcac ggcataagcg aggatgacgc tatcgccagc atccaccgga
     5761 tcttcaaaaa tgatgccgcg ccggaagata tcgccgccat cgtgattgag ccggttcagg
     5821 gcgaaggcgg tttctacgcc tcgtcgccag cctttatgca gcgtttacgc gctctgtgtg
     5881 acgagcacgg gatcatgctg attgccgatg aagtgcagag cggcgcgggg cgtaccggca
     5941 cgctgtttgc gatggagcag atgggcgttg cgccggatct taccaccttt gcgaaatcga
     6001 tcgcgggcgg cttcccgctg gcgggcgtca ccgggcgcgc ggaagtaatg gatgccgtcg
     6061 ctccaggcgg tctgggcggc acctatgcgg gtaacccgat tgcctgcgtg gctgcgctgg
     6121 aagtgttgaa ggtgtttgag caggaaaatc tgctgcaaaa agccaacgat ctggggcaga
     6181 agttgaaaga cggattgctg gcaatagccg aaaaacaccc ggagatcggc gacgtacgcg
     6241 ggctgggggc gatgatcgcc attgagctgt ttgaagacgg cgatcacaac aagccggacg
     6301 ccaaactcac cgccgagatc gtggctcgcg cccgcgataa aggcctgatt cttctctcct
     6361 gcggcccgta ttacaacgtg ctgcgcatcc ttgtaccgct caccattgaa gacgctcaga
     6421 tccgtcaggg tctggagatc atcagccagt gttttgatga ggcgaagcag tagcgccgct
     6481 cctatgccgg aggcgacgct gcgcgtcttg tccggcctac ggggatccag gtcggataag
     6541 gcgtttacgc cgcatccgac aatctgtacg tgaacaggaa gaaatctatg ttggccgggt
     6601 aaggcggagc cgctctccgg caaaaagaat caataacaat tatacgcgtg acccggcgcg
     6661 ggaaatgtcg gggcgctctc ccaagtgaca cactttcgag aggattcagg atggggcaat
     6721 catcgcaacc acatgagtta ggcggcgggc tgaagtcacg ccacgtcacc atgttgtcta
     6781 ttgccggtgt tatcggcgca agtctgtttg tcggttccag cgtcgccatc gccgaagcgg
     6841 gcccggcggt attactggcc tatctgttcg ccggattact ggtggttatg attatgcgaa
     6901 tgctggcgga aatggcggtt gccacgcccg ataccggttc gttttccacc tatgccgata
     6961 aagccattgg ccgctgggcg ggttatacca tcggctggct gtactggtgg ttttgggtac
     7021 tggttatccc gctggaagcc aacatcgccg ctatgatcct gcactcgtgg gttccaggca
     7081 ttcccatctg gttattttcc ctcgtcatta ccctcgcctt aactggcagt aatttattaa
     7141 gcgttaaaaa ctacggcgaa tttgagttct ggctggcgct gtgcaaagtc atcgctatcc
     7201 tggcctttat tttccttggt gcagtcgcaa ttagcggttt ttacccttat gccgaagtga
     7261 gcgggatctc aagattgtgg gatagcggcg gctttatgcc caacggcttc ggtgcggtat
     7321 taagcgcgat gttgatcacc atgttctcgt ttatgggcgc agaaattgtc accattgccg
     7381 ccgcggaatc cgacacgccg gaaaaacata ttgtccgcgc taccaactcg gttatctggc
     7441 gtatttctat cttctatttg tgctctattt ttgtcgtagt ggcgttaata ccgtggaata
     7501 tgccggggct gaaagccgtt ggttcttatc gctcggttct ggaattgctc aatattcccc
     7561 atgcgaaatt aatcatggac tgcgtgatat tactttccgt aaccagctgt ctgaactcgg
     7621 cgctgtatac cgcgtcaagg atgctctact ccttaagccg tcgcggtgat gcgcccgcgg
     7681 taatgggcaa aatcaaccgc agtaaaaccc cgtatgtggc ggtgttactc tccaccggag
     7741 cggcattttt aacggtggtg gtgaactatt acgcacctgc gaaagtgttt aaattcctga
     7801 tagacagctc cggtgctatc gccctgctgg tttatttagt catcgccgtt tcacagttgc
     7861 ggatgcgtaa aattctgcga gcagaaggaa gcgaaattcg cttgcgcatg tggctttacc
     7921 cgtggctcac ctggctggta ataggcttta ttacctttgt gttggtggtg atgttattcc
     7981 gcccggcgca acagttagaa gtgatctcta ccggcttatt agcgataggg attatctgta
     8041 ccgtgccgat tatggcgcgc tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata
     8101 atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg atggctatcg
     8161 ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat tacgaatgag
     8221 tttgctgaca tcgcgttatg cacttggcgt tgggccgtta cgggaagctc tttcgcaact
     8281 ggtggcggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc
     8341 agagcaggag ctgctcgata ttttcgacgc ccgcgccaat atggaagcga tgctggtcag
     8401 tctggcgatt gcccgcggtg gcgatgaatg ggaggcagac gttctcgcaa aagcgcatct
     8461 gctgagtaag ctggaggcct gtgacgccag cgagaaaatg cttgatgagt gggatctgcg
     8521 tcatcaggcg tttcatacag caattgtggc gggctgtggt tctcactatt tgctgcaaat
     8581 gcgtgaacgg ttgtttgatc tggcggcgcg ctatcgattt atctggctgc ggcgaacggt
     8641 gctttcggta gaaatgctgg aggataaaca cgatcagcac cagaccctga ctgcggcggt
     8701 actggcgcga gataccgcgc gcgccagtga gttaatgcgc cagcatttac tgacgccaat
     8761 tcccattatc cagcaggcga tggctggcaa ttaactactc ttccggaata cgcaacactt
     8821 gccccggata aattttatcc gggcttttta gcatcggttt attcgcttcg aagattttat
     8881 tgtacagatt agcgttaccg tagacctgtt tggaaatggc actcagagtg tcgccagact
     8941 taacggtata aaactggctg gcagtggctg gtgtcgccgt tttcacctga tcatcaacac
     9001 tggcaatacc ggaaatattc cccaccgcaa caaggatttt ctccttcgcc tcctgactca
     9061 ggccgtcacc agtgaccgtc gctttgccgt cggcaatttg aatgttcact ttatcggcat
     9121 caggtatacc ggttttgctc agatgctcct gcaccttctt cgcctgatcg tctttatcgt
     9181 gctgacctgt aaccgcgtcc cagagttttt ctccggcatc tttcacaaaa ttgaacagac
     9241 ccatagctac ctcattcgtt aacggaaaca ctcagaaagt gtagcagagg aacgcggcgc
     9301 gttaacgccg cgggggtgag cacaggttta atcgcgggtt tgcacccaga acgcgtgaat
     9361 caaaccgggg atatacccaa gcagtgtcag cagaatatta ataatgaacg cccaaccgaa
     9421 ccctttaccg agcagcacgc cgagaggcgg cagaatgatg gtgatgacga ttctccagaa
     9481 acccatatgt actccctata agaaaattac tcattgttta aaaagagatt ttatctctta
     9541 agcgtagtaa ttttagcgga gcctgccagt ttcagcttac gcttttactc tcatttacgc
     9601 tgctgatgct ggtatatgtt gcatttttag tttagagttt acttaattta gaaaatactt
     9661 aaataaatat gactgaactc gcgcaattac aggccagtgc cgaacaggca gcggccttat
     9721 tgaaagcaat gagccatcct aaacgattgc tgattctgtg catgcttagc ggttcccccg
     9781 gcaccagcgc gggagagctg acgcgcatta ccggactgag tgcctctgcg acatcacagc
     9841 atctcgctcg tatgcgggac gaagggctta tcgacagcca acgggatgcc caacgcattc
     9901 tatattccat taaaaatgag gcggtaaatg ccattatcgc caccctgaaa aatgtctatt
     9961 gtccgtaagg agtcactatg gctttgacaa ccatttcgcc gcatgatgca caagaattaa
    10021 tcgcacgcgg cgcaaagtta atcgatattc gtgatgctga tgaatatctt cgtgaacata
    10081 ttcctgaagc agatctggct ccattatccg tgctggaaca gtcaggtctt ccacctaaat
    10141 tacgtcgcga gcaaattata ttccactgcc aggcaggtaa acgcaccagt aataacgccg
    10201 ataaattagc agcgattgcc gcccccgcag aaatattttt actcgaagat gggattgatg
    10261 gctggaaaag agctggattg ccagtagcgg taaataaatc tcaacccttg ccgttaatgc
    10321 gccaggtgca gatcgctgcg ggtggtttaa tattaatcgg cgttgtactg ggttataccg
    10381 taaatagcgg tttcttctta ttaagtggct ttgttggtgc cgggttactg tttgcaggaa
    10441 tcagcggttt ttgtggaatg gcaaggttgt tagataagat gccgtggaac caacgagctt
    10501 gagaagcgac gccggacgcg ccctagcagc gacatccggc ctcagtaatt agatcaggaa
    10561 atcgtcgaga gatttacctt ctgccagcgc ctgagcaatt ggcttcggtg tacggccctg
    10621 accggtccag gttttagttt caccgttaac atcggtgaat ttatatttcg ccggacgcgg
    10681 ctggcgtttt ttaccagcgc gtggtgcagc agcagagcta ttacccaata actcttccgg
    10741 gttaattccg tcagccttca tcagctccag ccaggtgcta attttttcct gacgctcagc
    10801 cagttcacgc tgctgctgtt cttcttcttc acgtctttct ttagtgacaa ccctgaattt
    10861 ttcgagcatt tcttcaagaa cgtcaatgga gaattcgcga gccatcgcac ggagggtgcg
    10921 aatgttattt aaactttgta acattacgga cataataaag aaaacctttt aacgccaaaa
    10981 caaaaaagta tttcgtcgcg tagtgtaagc gatcacttat tatatttcca caaaatttaa
    11041 tctatttaca gtcctgcgcg agattatttc agctgttatt ttctgaaact ctcagaataa
    11101 tttcaagatt atatattagc cattttgatg agtaatttag attattggtg aacagagtgg
    11161 cgctctctgc tcgctaattc cgcaagcctg acaaaaccga agtgaataaa aatgcaatat
    11221 aacagaataa aagaaaacac ccctcactgg tgctttctgc tgttaatgta cattttctgg
    11281 cataaaaaat gctgtaaatg acacggtcac gacatacacc gaattctcta cgcttatagc
    11341 gttacctcac ccccaaactt tcattagtga aattaaaggc aaggtcatat ctaattgatt
    11401 taattgaaaa taaatcatat cggcatgtaa aaatccacaa tgtacaaaaa acgagccgtt
    11461 acggaatatt ttatctacaa aaactgacta aataaaaatt tttcactaat tgaatagtca
    11521 tagccagcga tatacgctat gcgaaaatgc agatgacaat gagatccact gctttcatct
    11581 ccattaacat cccattacgc ttttattaag gagcattagc atgttctcac cgcagtcacg
    11641 cttgcgtcat gcagttgcag atacgttcgc gatggttgtt tactgttctg tcgtgaacat
    11701 gtgtattgaa gttttcctct ccggaatgag cttcgaacag tctttttatt ccagattggt
    11761 agcgattccg gtgaacatct taattgcatg gccatacggt atgtaccgcg atctgtttat
    11821 gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa aatctggctg atatcctggc
    11881 ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg ttagtggtgg gcgcagactg
    11941 gcatcagatt atggcggcgg tcagttcaaa catcgttgtt tcgatgttga tgggggcggt
    12001 ttatggctac ttcctcgatt attgccgccg actgtttaaa gtcagccgtt accagcaggt
    12061 aaaagcctga ctcttctttt cgcgactggc atcgccagtc gcgtctttaa ctggcttcgc
    12121 caaataaacc attcaaataa cgttcaagcg caacacgcga actgaagcca tgcgggatac
    12181 cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa ttgctggtaa tgatcgcaag
    12241 ttttaatatc ggaagcgggt tcagccagtg ttgaactgcg ttcttttaat gttggatgaa
    12301 ttaccagagc ggtacgtccc attcgcgctt cgcgattaac atagacataa ttttccccac
    12361 ggcggtaacc atacgctttc tgggttacca catcgacagt aaaaccgact ttttcaagta
    12421 cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc tcgttattat caaccgcggg
    12481 cctaccttac ctgattgcgc attcacaacg ctttcagaaa agtccataaa gccgcgatga
    12541 acctcagtta agaaaattat ggtctacact gaaaattaca tcgaattcta atgggggatc
    12601 atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg cgtgcaggat atccagaatg
    12661 atgtcaatca attagctgac agcctcgaat ctgtattaaa gtcctggggc agcgacgcca
    12721 aaggggaagc tgaagccgca cgcagtaaag ctcaggcatt gctgaaagaa accagggcgc
    12781 gaatgcatgg tcgtactcgc gtccagcaag ccgcacgcga tgccgttggc tgcgcggatt
    12841 cttttgttcg tgaaagaccc tggtgtagcg tgggtacagc agctgcggta ggtattttta
    12901 tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa tgataataaa tacgcgtctt
    12961 tgaccccgaa gcctgtcttc ggggtttctt tttgcctggt gaatcacaaa aatcccccta
    13021 ccccgtcacg ctcatatcca gggtaatttc gaccactatt tgctatatat tgtgtggttg
    13081 aatctttttt caactacatc tagtatctct gtatcaacag agagacaacc cgacgcgtat
    13141 catcgcgccg tatcttcatt ttaaacggaa atacgaatca tgcgcattac tatttacact
    13201 cgtaacgatt gcgttcagtg ccacgccacc aaacgggcga tggaaaaccg gggctttgat
    13261 tttgaaatga ttaatgtcga tcgcgttcct gaagcggcgg aagcgctgcg tgctcagggc
    13321 tttcgtcagt tgccggtagt gattgctggc gatcttagct ggtctggttt ccgcccggac
    13381 atgatcaatc gtctgcatcc agcgccacac gcggccagtg catgagccag ctcgtctact
    13441 tttccagtag ctccgaaaat acgcagcgtt ttatcgaacg tttaggactg cccgcggtgc
    13501 gcatcccgct caatgagcgg gaacggattc aggtagacga gccttacatc ctgatcgtgc
    13561 cctcttacgg cggcggcggt acggctggcg cggtgccacg acaggtaatt cgctttttaa
    13621 acgacgagca caaccgggcg ttgcttcgcg gcgttattgc ttcgggtaat cgcaactttg
    13681 gtgaggcgta tggccgcgcc ggagatgtga ttgcccggaa atgcggcgtg ccgtggctgt
    13741 accgttttga actcatgggt acgcaaagcg atatcgaaaa cgttcgtaaa ggagtaaccg
    13801 aattttggca acgacaaccg cagaatgcct gacgcaggaa acgatggatt accacgcgct
    13861 gaatgcgatg cttaacctct acgatagcgc aggtcgcatt cagttcgata aagaccgcca
    13921 ggccgttgac gcctttatgg cgacgcatgt gcgtccgaac agtgtgacct tcagtagcca
    13981 gcagcagcgc ctgaactggc tggtcaacga aggttactat gatgaaagcg ttcttaatcg
    14041 ctactctcgc gactttgtca ttacgctgtt tgcccacgca cacaccagcg gttttcgttt
    14101 ccagacattc ctcggggcat ggaagtttta caccagctat acgttgaaga cattcgacgg
    14161 taaacgttat ctggaagatt ttgccgatcg agtcacgatg gtggcgctga cgctggcaca
    14221 aggcgatgcg acgctggcgt tgcaactgac cgatgaaatg ctgtcaggac gctttcagcc
    14281 agccacgccg acattcctca actgcggtaa gcagcagcgc ggcgaactgg tttcctgttt
    14341 tttgctgcgt attgaagaca atatggagtc gattggtcgg gcggtaaatt ccgcactgca
    14401 actgtcgaaa cgcggcggcg gcgtagcatt tttgctgtcg aatctgcggg aagcgggcgc
    14461 gccaattaaa cgtattgaaa atcaatcttc tggcgtaatt ccggtgatga aaatgctgga
    14521 agacgcattt tcctatgcca accaactcgg cgctcgtcag ggggctggtg cagtctattt
    14581 acatgctcat catcccgata ttctgcgttt tctcgacacg aaacgggaaa atgccgacga
    14641 aaaaatccgc attaaaacac tgtcgcttgg cgtggtgatc ccggatatca ctttccatct
    14701 ggccaaagag aatgcgcaga tggcgctgtt ttcgccctat gacgttgagc gagtttatgg
    14761 caagccgttt gccgatatcg ccattagcga acactatgac gaactggttg ccgatgaacg
    14821 catccgcaaa aaatacctca acgcccgtga tttcttccag cgactggcag aaatccagtt
    14881 tgagtccggc tatccctaca tcatgtatga agacacggta aaccgtgcta accctatcgc
    14941 cgggcgcata aatatgagta atctatgctc agaaattttg caggttaaca gcgcctcaga
    15001 gtatgacgag aatctcgact atgcccgtac aggccatgat atttcctgca atttaggttc
    15061 gttgaatatt gcccacacca tggattcccc caactttgcc cgcacggtag agactgccgt
    15121 acgcggttta acggcggtgt cagatatgag tcatatccgc agcgtgccgt ccatcgaagc
    15181 cggaaatgcc gcctcacacg ccatcgggct ggggcagatg aatttacacg gttatctggc
    15241 gcgagaaggc atcgcttatg gttcgccgga agcactggat ttcaccaatc tctatttcta
    15301 taccatcacc tggcacgcac tgcgtacctc gatgttgctg gcacgcgaac gcggtgaaac
    15361 cttcgccggg ttcaaacagt cacgctatgc cagtagtgaa tatttcagcc aatatctgca
    15421 agggaactgg cagccgaaaa cgacgaaagt tggcgaactg tttgcccgta gcggtattac
    15481 gttacctacc cgtgagatgt gggcgcaact gcgcgacgac gtgatgcgct acggcattta
    15541 taaccagaat cttcaggcgg tgccgccaac cggttctatc tcttatatca accatgctac
    15601 gtcgagtatt catccgattg tggcgaaagt agagatacgc aaagagggca aaacaggacg
    15661 cgtttactac cctgccccgt ttatgactaa cgagaatctg gcgctgtatc aggatgctta
    15721 cgaaatcggc gcagaaaaga tcatcgacac ctacgcggaa gcgactcgcc atgtcgatca
    15781 gggtttgtcg ctgacgctgt ttttccccga taccgccacc actcgcgata tcaacaaagc
    15841 gcagatttac gcctggcgca aaggtatcaa aacgctctat tacattcgcc tgcgtcagat
    15901 ggcgctggaa ggcactgaaa ttgaaggctg cgtctcctgt gcactttaag gaatatctat
    15961 gaaactctca cgtatcagcg ccatcaactg gaacaagata tctgacgata aagatctgga
    16021 ggtgtggaat cgcctgacca gcaatttctg gctaccagaa aaggtgccgc tgtcgaacga
    16081 tattcctgcc tggcagacat taactgtcgt agaacaacaa ctgacgatgc gcgtttttac
    16141 tggcctgacg ctgctcgaca cgctgcaaaa tgttatcggc gcgccttcgc tgatgcccga
    16201 tgcactcacg cctcatgaag aagcggtatt atcgaatatc agctttatgg aagcggttca
    16261 tgcccgctct tacagttcga ttttctcgac gctgtgtcag accaaagatg tcgatgccgc
    16321 ctacgcctgg agtgaagaaa acgcaccgtt gcagcgaaaa gctcagatta ttcagcaaca
    16381 ttatcgcggt gatgatccgc tgaaaaagaa aatcgccagt gtgtttcttg aatctttttt
    16441 gttctattcc ggtttctggc tgccgatgta tttttccagc cgcggaaagc tgaccaatac
    16501 cgcagatctg atccgtctga ttatccgcga tgaagcagtc cacggttact acataggcta
    16561 taaatatcag aaaaacatgg aaaagatatc tctgggacaa cgtgaagagt tgaagagttt
    16621 cgccttcgat ttgttgctgg aactctacga caacgagttg caatacaccg atgagctgta
    16681 cgccgaaacc ccgtgggctg acgatgtgaa agcgtttctc tgttacaacg ccaataaggc
    16741 tttgatgaat ctgggctacg aaccgttatt tcccgcagaa atggcggaag tgaatccggc
    16801 aatcctcgcc gcgctttcgc cgaatgccga tgaaaatcac gatttctttt ccggttcagg
    16861 ctcctcttat gtgatgggga aagcggttga aacagaagat gaagactgga atttctgagg
    16921 gtgttatttt caaaaatatc actacccgca gcagggaaat aattcccgcc aaatagcttt
    16981 ttatcacgca aatattctgt agtgatctac actgatactc tgttgcatta ttcgcctgaa
    17041 actacaatat tcaggcgttt tttcgctatc tatgacaaaa aatatcaact tttctcgatt
    17101 tgctctcagc ccttatatca cgggaaattc cggcgatttg ctcgcatcaa tattcatgcc
    17161 acatttgccc tcaggggttg cctcagattc tcagtatgtt agggtagaaa aaagtgacta
    17221 tttccattgg gtaatatatc gacataaaca aataaaggaa tctttctatt gcatggcaat
    17281 taaattagaa attaaaaatc tttataaaat atttggcgag catccacagc gagcgttcaa
    17341 atatatcgaa caaggacttt cgaaagaaca aattctggaa aaaactgggc tatcgcttgg
    17401 cgtaaaagac gccagtctgg ccattgaaga aggcgagata tttgtcatca tgggattatc
    17461 cggctcgggt aaatccacaa tggtacgcct tctcaatcgc ctgattgaac ccacccgcgg
    17521 gcaagtgctg attgatggtg tggatattgc caaaatatcc gatgccgaac tccgtgaggt
    17581 gcgcagaaaa aagattgcga tggtcttcca gtcctttgcc ttaatgccgc atatgaccgt
    17641 gctggacaat actgcgttcg gtatggaatt ggccggaatt aatgccgaag aacgccggga
    17701 aaaagccctt gatgcactgc gtcaggtcgg gctggaaaat tatgcccaca gctacccgga
    17761 tgaactctct ggcgggatgc gtcaacgtgt gggattagcc cgcgcgttag cgattaatcc
    17821 ggatatatta ttaatggacg aagccttctc ggcgctcgat ccattaattc gcaccgagat
    17881 gcaggatgag ctggtaaaat tacaggcgaa acatcagcgc accattgtct ttatttccca
    17941 cgatctcgat gaggctatgc gtattggcga ccgaattgcc attatgcaaa atggtgaagt
    18001 ggtacaggtc ggcacaccgg atgaaattct caataatccg gcgaatgatt atgtccgtac
    18061 cttcttccgt ggcgttgata ttagtcaggt attcagtgcg aaagatattg cccgccggac
    18121 accgaatggc ttaattcgta aaacccctgg cttcggccca cgttcggcac tgaaattatt
    18181 gcaggatgaa gatcgcgaat atggctacgt tatcgaacgc ggtaataagt ttgtcggcgc
    18241 agtttccatc gattcgctta aaaccgcgtt aacgcagcag caaggtcttg atgcggcgct
    18301 gattgatgcg ccgttagcag tcgatgcaca aacgcctctt agcgagttgc tctctcatgt
    18361 cggacaggca ccctgtgcgg tgcccgtggt cgacgaggac caacagtatg tcggcatcat
    18421 ttcgaaagga atgctgctgc gcgctttaga tcgtgagggg gtaaataatg gctgatcaaa
    18481 ataatccgtg ggataccacg ccagcggcgg acagtgccgt gcaatccgca gacgcctggg
    18541 gtacaccggc gactgcaccg actgacggcg gtggtgctga ctggctgacc agtacgcctg
    18601 cgccaaacgt cgagcatttt aatattctcg atccgttcca taaaacgctg atcccgctcg
    18661 acagttgggt cactgaaggg atcgactggg ttgttaccca tttccgtccc gtcttccagg
    18721 gcgtgcgcgt tccggttgat tacattctca acggtttcca gcaattgctg ctgggtatgc
    18781 ccgcaccggt ggcgattatc gttttcgctc tcatcgcctg gcagatttcc ggggtcggaa
    18841 tgggcgtggc gacgctggtt tcgctgattg ccatcggcgc aatcggtgcc tggtcgcagg
    18901 ccatggttac cctggcgctg gtgttaaccg ccctgctgtt ctgtatcgtc attggtttgc
    18961 cgctggggat ctggctggcg agaagtccgc gagcggcgaa aattattcgt ccactgcttg
    19021 atgccatgca gaccacgcca gcgtttgttt atctggtgcc aatcgtcatg
//
LOCUS       DAEVZA010000098        18870 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEVZA010000098 DAEVZA010000000
VERSION     DAEVZA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.098"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..231)
                     /gene="cas6e"
                     /locus_tag="LAF92_003466"
     CDS             complement(<1..231)
                     /gene="cas6e"
                     /locus_tag="LAF92_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBI9792418.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECR"
     gene            complement(213..959)
                     /gene="cas5e"
                     /locus_tag="LAF92_003467"
     CDS             complement(213..959)
                     /gene="cas5e"
                     /locus_tag="LAF92_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HBI9792419.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREKLSAPE
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDTLRNAYQQYQDHFRKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(970..2025)
                     /gene="cas7e"
                     /locus_tag="LAF92_003468"
     CDS             complement(970..2025)
                     /gene="cas7e"
                     /locus_tag="LAF92_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HBI9792420.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKVIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(2037..2573)
                     /gene="casB"
                     /locus_tag="LAF92_003469"
     CDS             complement(2037..2573)
                     /gene="casB"
                     /locus_tag="LAF92_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HBI9792421.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2570..4132)
                     /gene="casA"
                     /locus_tag="LAF92_003470"
     CDS             complement(2570..4132)
                     /gene="casA"
                     /locus_tag="LAF92_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HBI9792422.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4230..6929)
                     /locus_tag="LAF92_003471"
     CDS             complement(4230..6929)
                     /locus_tag="LAF92_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBI9792423.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGQPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKY
                     CSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(7123..7275)
                     /locus_tag="LAF92_003472"
     CDS             complement(7123..7275)
                     /locus_tag="LAF92_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI9792424.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(7540..8274)
                     /gene="cysH"
                     /locus_tag="LAF92_003473"
     CDS             complement(7540..8274)
                     /gene="cysH"
                     /locus_tag="LAF92_003473"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBI9792425.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(8348..10060)
                     /gene="cysI"
                     /locus_tag="LAF92_003474"
     CDS             complement(8348..10060)
                     /gene="cysI"
                     /locus_tag="LAF92_003474"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBI9792426.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(10060..11859)
                     /gene="cysJ"
                     /locus_tag="LAF92_003475"
     CDS             complement(10060..11859)
                     /gene="cysJ"
                     /locus_tag="LAF92_003475"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBI9792427.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            12178..12540
                     /gene="queD"
                     /locus_tag="LAF92_003476"
     CDS             12178..12540
                     /gene="queD"
                     /locus_tag="LAF92_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBI9792428.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            12618..13889
                     /locus_tag="LAF92_003477"
     CDS             12618..13889
                     /locus_tag="LAF92_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI9792429.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            13880..14140
                     /locus_tag="LAF92_003478"
     CDS             13880..14140
                     /locus_tag="LAF92_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBI9792430.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            14157..14732
                     /locus_tag="LAF92_003479"
     CDS             14157..14732
                     /locus_tag="LAF92_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBI9792431.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(14880..15740)
                     /locus_tag="LAF92_003480"
     CDS             complement(14880..15740)
                     /locus_tag="LAF92_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBI9792432.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQVDVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(15737..16516)
                     /locus_tag="LAF92_003481"
     CDS             complement(15737..16516)
                     /locus_tag="LAF92_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBI9792433.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(16494..17903)
                     /locus_tag="LAF92_003482"
     CDS             complement(16494..17903)
                     /locus_tag="LAF92_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9792434.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(17925..>18870)
                     /locus_tag="LAF92_003483"
     CDS             complement(17925..>18870)
                     /locus_tag="LAF92_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBI9792435.1"
                     /translation="IEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALC
                     YITEVTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQH
                     FTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGP
                     DKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSS
                     HSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHR
                     VHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT         4416 a         5158 c         4703 g         4593 t
ORIGIN      
        1 tcggcattcg atggtgaaag tttcactttc cgccgggcgt tcctgcgaga ggacaaaaaa
       61 gcgaaacgcg ccctggagtt cttcccgacg ataaagaaat tgcctttctt tgccgccagg
      121 gaagagatcc cacagccact gatgcatcac atattcaccg cgatccacca gatgcagcaa
      181 ctgcgctggg gaaagctggc cggtatgtaa ggttattctt gagaggtaca tggttcctcc
      241 ttgctgagcc acggcccctg attgacggtg cgttccccaa acaaccactg ctgacgattt
      301 aaaggaacat cccgacggcg taatattttg ctcgcgacca ggccgtcgtg ttccccttcc
      361 caccagcatt catcctgaag ttttgggagt gagactttca gtttgcggaa atgatcctga
      421 tactgttggt atgcgttacg taaggtatcc gacgcgttgc cttcgagtag taacggcgca
      481 agtggtaacg ccagcggatg actttttcgc cccagataaa gcggaaaaac cggatggcgt
      541 aaaccgtcct gcaactgttc gaggctgtaa ggcgcatcgg gggttgttgc caccgccacc
      601 atccaccagg catcggtgta gtagtcgcgc cgggagataa tcgcgctcaa aagctcaggg
      661 gcgctcaact tttcgcgacg gctgaaataa cgtgctttac gcacctcttt tggcatctgg
      721 accgtgtgat aatcccgtgc ccagcgcggg ttacggctgg cgcaaaccac cagtgaatag
      781 tggcgattaa acgcgtttaa gcgttcggta tcatcacgcc gaatccctac cccggcagcc
      841 agtagcccca gcaatgctga gcgcgaagga agttcatggg tatgacgaac ttcacctgga
      901 gcatcgaccc cccaggacgc catcggccca tgaagctgaa aaatcagata ttggctcata
      961 agccgcccct tacgcgcaga taaagtccag cacgtccttc atgcttccct gtttgttcat
     1021 cacgtcaaaa cttgcgcatt cggtcttctg ttcgtagacc gtattcatgt tttcgcgaag
     1081 cgttgtaata cgctgcaccg ccacatctaa ctgacgtgtg ccattaatgg gttcatagaa
     1141 agccgccgcc agagagcgtg gttgatcggt gcctttttct gccagcgccc aggaggcgta
     1201 ggcacggctg gcaaagctgt tctgtttgcc ggttggggag actttaagtg cggcttccgt
     1261 aaaggcacgt atggtctgat tagctaacgc ttcgtcaccg ccaaggtttt cgaccagcag
     1321 atctttatca atgcagatat aggtgtagaa cagtgcagaa ccgaatccgg tttcaccaag
     1381 atgcccggca ccggcatctt cagaagcctg gcgcaaatca tcaacggcgg tgaaaaaatc
     1441 atcttcgaca atcgtttcac tgacaccaaa tgcgtgcgct acctggcagg cggcttcaac
     1501 attaaactcg ggtttattcg ccagcatacg accaaacatg gcaatatcta ccgccatacg
     1561 gtctttgcgt aacaacgcga gatcttcctc ttttggcgcg cgcttttctt ctgcaagctg
     1621 atgggccagc gcttttacgg cgtcaaattc tgccgggctg atatggacta actgttcagt
     1681 ttcggcgtta gtgagcggat cttttggttt tttgtcgttt ttagctttcc caagataatc
     1741 tgcaattttt gccgcccatt cgatgacttt tttctcttcg attcctttct caatcaggat
     1801 agttgccgcc tcacgcgcaa tacgcccact gcgaatacca atatggcccg ccagtgcctg
     1861 ttcaaaaagt gcagaagtgc gccacgcacg tttcagactt tgcgaggaaa cgcgcagacg
     1921 cgttgctcca cccagcacca cagtttttgg cgcaccggta tcatcacggt taaggttggc
     1981 agcagggtaa gcggttaaca aatgaagctg aataaatgtt gtcatgagat agtcctttat
     2041 tgattttgtt cgttatcggc gtcgccagcc tggtaatatt ccagcgccca gcggatacgg
     2101 ataaattcgg ttggccgctg ctggcggcgg tgatgattga gcaggtcgtc gctctcctgg
     2161 caccaacgga agaccccctc tgccagagag tcaagattga cggaaccgtt taataatctg
     2221 actgcgcggc gtaactggcg cagtaattca tccggtgttt ttactgccga cagacgagta
     2281 aaacgcccct ttgacattac cgccgccagc tgcgcggcaa aaggctgccg ttcatcaata
     2341 gctttaacat tggcgctaac tgcggctatc agcgccagtg cggtgatacg ccactcaggt
     2401 tcatcctgcc actttatttg tctgttcttc agaaacaggc gaaatccatc cgtcagacaa
     2461 acatcattaa ccgtcgtact acgccgcaga ctggcacgtt cgccacgttt ctcctgcaat
     2521 tcctcgtgcc atttgcgcag cgtggctttg tgctcctctt ttacaatact cattcagcag
     2581 cctcctgctt tttctccctg gcggctttag cactttgctt ctccgccgat gttgtgaaat
     2641 atttcttgcg cgcggtcata acgcgtttca aatcaacagg ctcatatgga ttggtgaata
     2701 cacgctcgtc aaaatcctga cgcgcgaata accaaatttc cttttgccat ttgccgagta
     2761 attcgtccgc atcctgacct tcttcaattt ggcgcactaa cctcaggaag cgatgctgag
     2821 ttttgttcca gaagtcgata tccacaaaac tgaaatcacc ccgtgcacct tttggatcgg
     2881 agaaccacgc ttctttcaat gcacttcgta acagacttag aatccgtgaa gccgtttgcg
     2941 cagccagacg cagtttcggt atctggcctt cttttttctt gagcagcagc gggaaatggt
     3001 gttcgtacca acagcgcgct ttcatgttgt cgaaatcata gccaaatccc cacaggccta
     3061 cttttgcctg tttcagactg ctggcattaa agagtttcac caccagcgcg ggaagttccg
     3121 tattgttttc tgatttaccc gtttcgataa ggcctaacca gtcgcgccag attaaaccgc
     3181 ccggttgtgg tttgaccgag taaaactcgc caccctcttt aagtggtata cggtaaggcg
     3241 ttaagggatg ctgccacatg gcataattcg caccgtaatt tttggtcgtc atcaggctca
     3301 ggagcgcgtc gctctgttca ccgcaaatat cgcagttgcc gactgtcgtg gtattaaaat
     3361 caatacgaat acggcgcggc attccccagt aagcctggag tttattgacc tgatcatggg
     3421 ttaccaccgc accggccagt tcgctggttc gcgtcgggcc aagccagggg aaaaccagat
     3481 cgtcaaattt tttgggtagc ggtaagtcgg cttcatcctg cggcatcaca ttgggccaca
     3541 gtttgcgcca caagggcgtt tgttgattgc cttgatattc ctgtaattca atcagagtgg
     3601 tcatcggccc accgccgcgc aaaccggtgc gatagccttt gccgcctgat ggcgcattta
     3661 actgtaggga gaacagagct aacgcggaac aatgagggca tacgtgttca gtcacgccac
     3721 gcttaataaa gtggtcttta ttaaacttcg ttgtttgagc gccgggaatc tcaggcagta
     3781 gcgaagcgac ctgaacttta tctcccttga gcgcctcgaa atcctgcata aatgaagggg
     3841 aatctggccc aaactggaaa gcatgttcta atgactgcaa tgcttcccgt agcttttcag
     3901 cttccagtcc gtcttcccag atatcatccc aacgacgatg atcttttggc gcgaagctgg
     3961 tttgtagtag ccccagcaaa aactgccata ccgcaccctg gagatctgcc cgcggcgcag
     4021 cgatatcgac aacattttca tcagccagat cgactggtgc cagcttgcct gtcgttccgt
     4081 ctttaaaacg aacaggcaac cacggggttg tcagaagtga aaacgagttc atacgcatcc
     4141 tctcgtaaaa aagaccatcc caggtcggtt gtccatcttt ccatcctttt cggtgtatca
     4201 actattcaga gagtcctgtc gcagacatct caaccaacct tgccaatcaa tccttccgta
     4261 gccgaatacc cgcaactttc gtcatcgttg actaaaatca cgtttgccat ctccggatct
     4321 tgtcgctgtt caatgcacca cagcctgaac gcctctccct ccagtaaaga aaactcatcc
     4381 cgatgttttt tccaccagct tcggcgcact ctgacaacgc tcatttccca tgcgtgagca
     4441 ccggtggcat aaggcttcac cacaccgtca atacaggtag ccagccacag ggaaacagac
     4501 tcctcagcca gacgtgtcga cagtttttcc gggaggtaat cgttgatatt ggcggcatag
     4561 ccgggcctga agttcagcac aaacttttta gccattgcgc gatcgcagta atatttgccc
     4621 acttgctcct gctcgctgcg ggcaaatcct tccggcatta ccacgtcctc accgtagacg
     4681 gattcaataa gaagccgggc tgcgtgtggc atctgaatag caccttgctc acgcagcaca
     4741 cgctgcgtca gccagattcg cccatgatcg ggatagacaa atgcactgtt acgcatggca
     4801 ctgccaaacc attcgtcgcc aggagagtcg tcccagacgg gagccagaat cagcaattca
     4861 gggggggaac gctcgtcctt tccgtcacgc tttaactgac cattaatatc gcggatatgc
     4921 cgctgtaatc gtcctgctcg ctgaatcagc aaatcaacag gggccaggtc ggagatcatt
     4981 tcgtccaggt caatatccac gctttgctca attacttgtg tacaaacaat gacttgcgac
     5041 gctctctgta aactacaata tttaccaaag cgtgccagtg tctccgtttc aattcgctgg
     5101 cgatcgctaa aagcaaagcg gctatgaaaa agtgaaaggc tggaagcagg aatgacgccg
     5161 cgggcaagca gctgacgatg aaccttaata gcgtcatcga cagaattccg gatccaggcg
     5221 atgcatttcc cctgacttac cgccgattcg atacgcgcaa tacactcttg ttcactatga
     5281 agccaaccca cgctgacgct acgctcaacg tctttgcgcg tcgctacccg gtgtgagttc
     5341 acatcggatt tcgtgacatg cgtcagccag gggtaatcat ccttttccag gaacggagct
     5401 tcttgctggc cctctgtacc ccgcgcaaag gcagcgacga gtttgtcgcg ctgctgttga
     5461 gataacgtgg cagaaagcaa aataacgctg tttccgccac gcgcctgccg ctcgatcaac
     5521 ccttcaagaa tgcacgacat gtaagcgtca caggcgtgga tctcatcagc cagcaggatt
     5581 ttgttactca gccccagaag ccgcagatta ttatgtttga acggcatcac cgccatcatc
     5641 gcctgatcca gcgtgccgac gccaatttca gccagcaacg ccttcttgtt actgtcggca
     5701 aaccaggccg cacatccctg actgaacgtt tgttcatccg gttcttctga cccgactaaa
     5761 tcaccagacc agagtgattc attaaagcgg tccattaatg tgcgggcact atgtgccagc
     5821 accaggctgg ggcgggattc tggcgaatag aaagcaagcc aggttttgac cagccgatcg
     5881 tacatggcat tggccgttgc cattgttggc agaccaaaaa acagcccctg agctttcccc
     5941 gcagctatca acctgtgcgc caggataagc gccgcttctg ttttacctgc gccagtcaca
     6001 tcttccagaa taaataactg cggtccttgc tggctgatat ccagatccag caccttttgc
     6061 tgtaacggtg tcggattctc aataaaagga aacagcgtat taattccgtt gaaaggcgcg
     6121 acttttgctt ttagaggaaa gacggttaag gcgttttgag cctgaattaa agttttctgc
     6181 cagtaacctt taatatccat tgggtgtgcg acgcgtggaa aaaatcgcgt tgacgaaccc
     6241 gtccagtctg cgagcacgac ggttgcagag atataccagg aaaggtgttt tataagttca
     6301 atgccctcat catcatccca gaatgcgggg atctctatga gcggaaacag tggtttgatt
     6361 tcaaggagaa aatctcgtgc tgcagctttg tcttcaggca gaaaattatc caattcatcc
     6421 atacgatcag ggggctgacc atgatgccct gtcgtcacgg gcatccacat ttctatcact
     6481 cgcctaagtt tgcgcggaga gagcgaagat gaaggaagta actcctgaca ttcacttaaa
     6541 taatgattcc acaaccagta gcccagcgtt gaatgagaga ttttttcgta attttttctg
     6601 gcaccttcct gaatcttgag ttcagggggc aggtaaagtt gttgaaaaga gcgggcaaat
     6661 tttccaatat cgtgccagca cagcaaccag gcgaaaaatt gagccgcctg ttccttgtca
     6721 gaaatcccta attgacgaaa ataatcagcc agcccgaagc aatttctttt aaccattaaa
     6781 tagcccatcg cggctacatc cagcgaatgc cagcaaagaa ggtgatagtc gtcgcccccg
     6841 tctttctcgc cgcgtcgggt ttttccccag aaatcaaaaa aagtcacaat atttttatcc
     6901 ttcagtaaac ttaaaggata tttacgcata ttttaaaaat tatctgtgat atatatcagc
     6961 caataaaaca tttacattat gaataaatta atgattttca tttgaaattc ataataataa
     7021 atagagacta tatattcaca taaaaaatat aaattttaaa aaagggtgca ggagaccaaa
     7081 ccatcacaat tcagtacgcg gggttttacc cccgcgtggt ggctacttct tcgattcgta
     7141 agcgagaact gccttaaact ccataccacg ttctctgata ctcaactcac acaacgagtc
     7201 acctaccatc agcgtgaatc ccagtactgt acaacacagt acgatgattg ccacaagggc
     7261 atattttgtc agcatgtctt gcctccctgc aaagaagaga ctaacattcg aattgtttgg
     7321 gttcgaacgt aagcctcagg ttgatagaaa tatcgcctgg ggcttttgtc catctggaac
     7381 ctcgcgaatg cttaacgcca gacagcctca agcacccgac gccattctat acctgataat
     7441 tcttccagcg gcgttcacgt ttaaaatcag gaatactgct cgcaaaataa aaacgccccc
     7501 atcatttctg acagaggcgt ttaatttatc cggcaatatt tacccttcgt gcaatccaca
     7561 ttcccttttt aagccaaaga aacgtgtttc ttcttccgac atgccaggtt cccatttacg
     7621 ggttgtatgg gtatcaccga ccgataaata tccttcatcc cataatgggt gatatttcag
     7681 gccatgtttt tgcaggtact gataaatagt tcggttatcc cagtcgataa tcggcagcac
     7741 tttaaatacg ccacgctgaa ttgccagcac cggtaaattg gcgcggctgc cggattgttc
     7801 gcggcgcagg ccagcaaacc acgtttgcgc attcagttct ttcagcgccc ggttcatcgg
     7861 ttcgactttg ttgatgtcat tgtacttttc aatgccttca acgccctgtt cccacagttt
     7921 gccgtagcgt gcttcctgcc aggcggcgct ttcggtagcg cggtacactt tcaggttgag
     7981 cttgagtttg tccgttaact cgtcaataaa gcggtaggtt tccgggaaca gatagccggt
     8041 gtcggtgagg atcaccggaa tatccgggtg aatctgattc accagatgca ggctcaccgc
     8101 cgcctgaatg ccaaagctgg aagaaagtac atattcaccg ggcagattat ccagcgccca
     8161 ggcaacgcgg ccttcagcgt ccagtttttc cagttcggcg ttagtttctg ccagcgccag
     8221 aatgcgatct accttcggca gttcgttcag ggcgtttaga tcgagtttgg acatagattc
     8281 ctcactgttt gccttgcctg atgcgacgct cacgcgtctt atcaggccta caagaccggg
     8341 ctgatggtta atcccacaaa tcgcgcgccg gatcgagcac cgggcgaatg atgcccgcac
     8401 gcaccgtaaa gtcgccgaag ccttcacccg cttcgcgctc tttcgcccag cgccctatca
     8461 gttcatcaag cgacgccagg atttccggct cggtgatgtt ttctttatac atccgtggga
     8521 tacgtgtccc aatgcggttg ccgccaagat gcagattgta gcgacccggt gctttaccca
     8581 ccaggcccac ttccgccagc atcgcgcgac cacaaccgtt cgggcagcct gttacacgca
     8641 tcacgatatg ctcatcgctg acaccatgtt tcgccattaa attatcgatg ttgtcgataa
     8701 aagacggcag gaaacgctcc gcttccgcca tcgccagcgg gcaagtcggg aatgacacgc
     8761 aggccatcga gttttcacgc tgcggcgtga cggcattcat taaaccgctc tctttggcga
     8821 tcttctcgat cttcgctttc tcgctttccg gtacaccggc gatgatcaga ttctggttcg
     8881 ccgtaatgcg gaaatcgcct ttgtggatct tcgcgatctc cagcaggccg gttttcagcg
     8941 gacgccccgg ataatcaagg atgcgaccat tttcgataaa cagcgtcagg tgccagttat
     9001 catcaatgcc cttaacccag ccaatacgat cgcctcgtcc ggtgaactca tatggacgga
     9061 tcggttcaaa tttgatcccc gcgcgacgct ccacttccgc tttaaacgtc tcaaccccca
     9121 cgcgctccag cgtgtatttg gttttggcat ttttacgatc ggttcggtta ccccagtcac
     9181 gctgagttgt cacgacggct tcggccaccg ccagcgtatg ctccagcggc agatagccaa
     9241 actcgctcgc cgtgcgggcg taggttttct tgttgccgtg ttcgatagaa agcccaccgc
     9301 ccaccagcag gttgaagcct accagcttgc cgttttcggc gattgccacg aagttcatgt
     9361 cgttggcatg cagatcgata tcgttctgcg gcgggatcac taccgtggtt ttgaacttac
     9421 gcggcaggta ggtctgacca aggatcggtt cttcatcagt agtggcgact ttttcctggt
     9481 cgagccagat ctccgcatac gcgcgggtac gaggcaacag atgctcagaa atcttcttcg
     9541 cccactcgta cgcttccgcg tgcagctgcg actcgtaagg gttcgaggtg cagagtacgt
     9601 tacggttcat gtcgttagct gtcgccagcg catcaagacc gaccgagtgc agcatctggt
     9661 gcaccggttt gacgttcttt ttcagaatgc cgtggaactg aaacgtctgg cggttggtca
     9721 ggcgaatgct gccatagatg gtgttttcac cggcaaattt gtcgatcgcc tgccactgtt
     9781 tagtggtaat caccccaccc ggcagacgac agcgaagcag catcgcgtgg cgcggctcca
     9841 gcttctgttc agcacgttcg gcgcggatgt cgcggtcatc ctgctgatac atgccgtgga
     9901 agcggatcag caggaagtta tcgcccttaa agccgccggt cagaccgtcg tttaaatctt
     9961 ccgcaatggt gccgcgcagg tagttgcttt caagcttcat gcgctcggcg tctgtcagtt
    10021 ttccttcgac cactaaaggc cctggatgtt tttcgctcat tagtagacat ctcgctgata
    10081 acggcgctct acgcgcagct cacttaaaaa ttcatccgcc gcttcggtgt ccatgccacc
    10141 aaattcggca atcacttcca gaagtgcctg ctcaacgtct ttcgccatgc gattagcgtc
    10201 gccgcagaca taaatgtggg caccatcatt gatccagcgc cacagctccg cgccctgttc
    10261 gcgcagtttg tcttgtacgt aaactttttc tttttggtcg cgcgaccagg caagatcgat
    10321 acgtgtcagc acgccatctt tgacgtagcg ctgccactcc acctggtaca ggaagtcttc
    10381 cgtaaagtgc ggattaccaa agaacagcca gtttttacct ggcgcttcgt cggcggcgcg
    10441 ttgctgcata aaggcgcgga acggcgcaat accggtgcct gggccaatca taatcaccgg
    10501 ggtttctgga ttggctggta ggcggaagtt atcgttatgt tcgataaata cgcggacttc
    10561 gccctcttct tccacgcggt cagcgaggaa gctggaggca ccaccggcac gggcgcggcc
    10621 ttccacgtcg taacgcacca caccaacggt gacgtgtact tcgttctcga cttccgcctg
    10681 cgaggaggcg atggaataca ggcgcggggt cagcgggcgc agcagattaa tcagcgcttc
    10741 ggcatccagt tgcgccggag agaaacgcac catgtcgaca atcggcgtcg tcgcggcgta
    10801 atgctgtaac ttcgctttat cgcccaccag cggcagcagt gtttcgctgc gggtaagcgt
    10861 ggcgtaattc tcaacaatgt tggcggtgtt gacggtcagt tcgaagtgcc actgtagcgc
    10921 ttcgttcaga ggcaacgttt tgccctcgac ggtgacaggt tcatcgcctt tcagccacag
    10981 cagttcgaca agttctttca ccagtgccgg atcgttctga taccagacgc ccagcgcgtc
    11041 acccggctgg taacgcaggc ccgagtcacc taagtcaatt tcgatatggc gaacgtcttt
    11101 ttcagagtta cgcccggtaa ttttctggtt aacagagagg ctagccacca gcggcgcgtc
    11161 tttgctgtac gggctggtgt ggatttcatt taccgcgcca gtagcgacgg attgcgaagg
    11221 tgccgcgaca ggcgcacgcg atttaagcgc atcaaccacg cgggcgcgcc actcgctggc
    11281 agcagcctgg tattcaacat cggcatcgac acggtcgagc aggcgttcac cacccagttc
    11341 cgccagcttg ctgtcgaaat ctttcccgga ctggcagaaa aattcataag agctatcgcc
    11401 gaggctaaac acggcaaacg cggtgttttc cagctttggc gcttttttgg agaacaggaa
    11461 cttatgcagc gcgacggctt cttccggcgg ttccccttcc ccttgcgttg acgtcaccac
    11521 gataagcagt ttttcgctgg cgatttgttt gaatttatag tcgcccgcgt tcaccagctt
    11581 aacgttcagt tttgccgtca ataaatcatc gcgtaatgct tcagcaaccc ggcgcgcatt
    11641 gccggtttgc gaagcagaga taatagttat acccggcatt tctgcggctg gcgctggcgt
    11701 cgctgcaagc gcagcaggct gctgattgag cacgccccag aaatagccag aaacccaggc
    11761 aagctgggtg ggagttaaat cggtcgtggc cgcctgaagg cgtgccagtt gctccgggtt
    11821 caacggaagc aacgcggaag gtgggacctg tgtcgtcatg cgtcgttatg ttccagtaag
    11881 caaagctgtt tctgcgccct gtcagcgccc ataaaacaga agagaaggta aggttaacgg
    11941 ggcaaatggt gtggattaaa gacgggatag cgataactaa taaccaaatc gactaacctg
    12001 ttttagcaat agtctttaac aacaaaatag attaaccaac ccaatgaaaa acaaatgaat
    12061 ttagccaatc attaagataa atcagcgatt ttgcgcaaca aagtcgtttt agataatgcg
    12121 aaaaaacagc ctttccggta ctctacggcg gttttatcgt cctgtagaga aattatgatg
    12181 tccaccacgt tatttaaaga tttcaccttc gaagccgctc accgcttacc acacgtcccg
    12241 gaagggcata aatgtggtcg cctgcacggg cattccttta tggtgcgact ggaaattacc
    12301 ggggaagtcg atccgcatac gggctggatt atcgatttcg ctgaactaaa agcggcgttt
    12361 aaaccaacct acgagcgcct cgatcaccat tatctcaatg atattccagg tctggaaaac
    12421 ccaaccagcg aggttttagc aaaatggatt tgggatcagg ttaaacccgt tgtgccgctg
    12481 ttaagtgcgg tgatggtaaa agaaacctgt accgcaggtt gtatctatcg cggcgaatga
    12541 taagagtgtg tcggcggtca atttccctta agtaacgcta tgttagggtg ttgtgttctg
    12601 gatatctggg gcatgacatg gaagacgact gcgacattat tattattggt gccggtattg
    12661 caggcaccgc ttgcgcgtta cgctgcgcgc gagcgggttt atccgttttg ttactggaac
    12721 gcgctgaaat ccccggcagc aaaaatcttt ccggcgggcg gttatatacc catgcactcg
    12781 cggaactcct cccgcaattt catctgaccg cgcctcttga acgacgcatc actcacgaaa
    12841 gcctttccct gttaacgccc gatggcgtaa cgacgttttc cagcttacag cccggcggtg
    12901 aatcctggag tgtattacgt gcacggttcg atccgtggct ggttgccgaa gccgaaaaag
    12961 aaggtgtcga atgcatcccc ggagcgacgg tggatgcact gtatgaagaa aacggcaggg
    13021 tgtgtggtgt catttgtggt gacgatattc tccgcgcccg ttatgtggtg ctggcagaag
    13081 gtgccaacag cgtcctggct gaacgtcacg gattagtgac tcgtcctgct ggcgaagcga
    13141 tggcgttggg gatcaaagaa gtgctgtcgc tggaaacatc cgctattgaa gaacgttttc
    13201 atctggagaa taacgaaggc gcagcgttgc tgttcagcgg caggatctgt gatgacttac
    13261 ccggcggcgc atttctttat actaatcaac aaacgctctc gttagggatt gtttgcccgc
    13321 tctcttccct tacgcaaagt cgtgttccgg caagcgagct gctgactcgc tttaaagcgc
    13381 atccggcagt gcgcccgctt atcaaaaaca cggaatcact ggagtatggt gcgcatctgg
    13441 tgccagaagg tggcttgcac agtatgccgg tgcaatacgc cggtaacggc tggctgctgg
    13501 tgggcgatgc gttgcgcagt tgcgtcaata ccggaatttc cgtgcgcggc atggatatgg
    13561 cgctgactgg cgcgcaggcg gcggcacaaa cgctgataag cgcctgccag caccgcgagc
    13621 cgcaaaatct gtttgcgctt tatcatcaca acgtagagcg cagcctgcta tgggatgttc
    13681 tacaacgtta tcagcatgtt ccggcgcttt tgcaacgccc tggctggtat cgggcgtggc
    13741 ctgcgttaat gcaggatatt tcccgcgatt tatgggatca gggtgataaa cctgttccac
    13801 cgctgcgcca gttattctgg cgtcatttac gtcgtcatgg cctgtggcat ctggcgggcg
    13861 atgttatcag gagtctgcga tgtctgtagc ccataatctc tggcgcgttg ctgatgcgcc
    13921 gcacattgtt ccggctgact ccgttgagcg tcagatggta caacggttga ttaacgcctg
    13981 tccggcaggt cttttttcgc tcacaccgga aggtgactta cgcgttgatt atcacggctg
    14041 cctggagtgt ggcacctgcc gtttgctgtg cgacgaatcc acactacaac agtggcgcta
    14101 tccgccttcc ggattcggca tcacctaccg ctttggataa ataataagga taatttatgc
    14161 ccctcttaca cctgctccgc cagaatccgg tgattgctgc cgttaaagat aatgccagcc
    14221 tgcaactggc aatcgactct gaatgccagt ttatttccgt gttgtacggc aatatctgca
    14281 ccatcagtaa tatcgttaaa aagattaaga acgccgggaa atatgctttt attcatgttg
    14341 atttgctgga aggcgcgtca aataaagaag tcgtgattca gtttttgaaa ctggtgaccg
    14401 aggcggacgg cattatcagt accaaagcct caatgttgaa agcggcaaga gcggaaggtt
    14461 ttttctgtat tcatcgcctg tttattgttg attcaatttc gtttcacaac attgataagc
    14521 aagttgcgca atcgaatccg gattgtattg agatcctgcc aggctgtatg cccaaagtgc
    14581 tgggctgggt gacagagaaa atccgccaac cgctgattgc cggtgggctg gtgtgcgatg
    14641 aagaagatgc gcgtaatgcg attaacgcgg gtgtcgtggc gctttccacc acgaataccg
    14701 gggtctggac gttagcgaaa aaattacttt gacgggataa tcgtaaccaa ttgaatttgg
    14761 tttgatttgt aggccgcacg ccacatccga cattcagcgc ctgatgcgac gcttgacgcg
    14821 tcttatcagg cctacaagtc cccaggccag aaccgtaggc aggatcatcc ggcaacacgt
    14881 tattggcaat ctgcgtgaat gtttgtcacc agtgcctcca gcaccacttt ccagtcatca
    14941 accacgccaa catctacctg tgaaaatacc gcagcgctgg cgtcatgatt aatcgccacc
    15001 acaaacttgc tattgcgtac gcccgccatt aacgctgctg cgccagaagc gccaacaaca
    15061 atacacacct caggtgccag cagatgtccg gaaataccaa tcactttttc agcatcaacg
    15121 ccaccgttca tcactcgcgc ccgactgtag cccacttcgg ctcccagctt ttctgccagc
    15181 atagcgattt cctggttatc tgcctctccg ccctgcccca ccaccagcac ccgtctggcc
    15241 tcagctaatg ggtcgcgagt aacatttttc agatcctctg tgctaaccag ccagtcggga
    15301 agcgcacccg ggacaatgtt cagttgttgc attccagagg gcaacgtggc gtttttcgct
    15361 gctcccgcct gacgcgccag ggaaaggcat agcgggcgtt tttctgtttg cagcgtcgcc
    15421 gttagcgcat ttccccagtg ggatttgcgc acgcttacgg ttggaatatc taacgaagtc
    15481 acctggcaga tgcttgcacc atgtaaacgc caggccagcc gggtcgacag ttcatcgcca
    15541 aacgttcccg gcggaaagag aacaacgtcc gcaggcgttc gctgccactg ttccaccaac
    15601 gcatccagca cctgttccgc cactacaggc tgaggttcaa tctgccagtg cgccagggta
    15661 caaccgctga aatcctgcgc cgccagccag cttgctattg cggcattctc ctgatttatt
    15721 gtcacaattg cgatattcat ggctgcatcc tttggcgtaa atagtcctgc catagctttt
    15781 gagctttttc tgctaccgtt tgcccatcaa tcagcgtcgc tccgcgtcgt tgctctgctc
    15841 ttgccagttg cagacactgc atcgctggca tttcggcagc aacagtttta cggataattt
    15901 ctgctttccc cgcagccatt cgctggcgca ttcccggtac tggcaaggcc acttcaccgc
    15961 actgacgcac ggcaatcacc gcaggcaagc gaacccggca acaacgcaaa ccatgttcag
    16021 tacgctgttc gagggtgata aacagcgcgt cgagagtgaa acgctccacc tgggtgaagc
    16081 agggccagcc cagcatttcc gccagcaaaa atggcgtttg cccattctgc ccttcgctgc
    16141 tttggcagcc ggtaattatc agatccagcg gattctgatg ctgccattcg gcaatatggc
    16201 gagcgacaaa ttccggcgca aagcgcagat ctgccgccgt ctccagcaat acagcttcct
    16261 caaacccaag agccatgaga tagcgcagcc agtgcaatgc ccgttcatcc cccatgctca
    16321 acgcggttaa agacatcggc gtaccgtttt ttctctgcgc caacagcagc gcggcagcgg
    16381 cctgttcatc agcaccgagt aaacttcgca gtagcgaaat atccggtccg cttttaccct
    16441 gagccgccgc ctgccactct ttttccgcca gcattccggc atccggttcg gctttaaacg
    16501 ctaacagaat gttcattcgc acctcctaca tttcccgccg ccaccagcgg gagggcttta
    16561 gtttctggtg cccatagcca ggtcacaaca aaaccaacca acaacactgt cgccaggagc
    16621 aataaggtga cttgcattcc ccactgcgcc agcacccacg gcagcaggcc agtacttacc
    16681 gccgcgccaa gtcgactcat ggcggtggca aaaccgacgc ccagcgagcg aatgtcagta
    16741 ggaaaacttt ccgcaggcaa aatgcccacc agattactga ctgccgaaat ggtggtgctg
    16801 aagagaacaa aaagcagcag cgttaatgaa ctgccggaag gcaaacaggc cattactacc
    16861 agcgttgccg ccagcagcaa aaaacttccc agcaaaaatt tgcgatgtgc cagcaggtgc
    16921 gtcagaacta atcccagcag cgcgcccaca attaacaacg cattaagcat caggctggca
    16981 gtcagcgcat cttccagacc aatagtctgg gcgatagttg gcagccaggt ataaatcaca
    17041 aaccatggga ttacgaggca gacaaagaag acgctgttaa acgccgtgcg ccgccagtaa
    17101 cgcgaagaga acaaggtttt gatgtgttta tgggtcgccg ttaccacttc atcgcccagt
    17161 aaaacatggg gaccaaaata gcgatgcacg atagcgtgag cttctgcaaa acgcccctgg
    17221 cgcagtagcc agcgtggtga ttctggcgtt ccccagcgta ataacgtaat caacaacgcg
    17281 ggcagagctg ccgatgccag tagccagcgc caggcctccg ggttttcgga aataaagtga
    17341 tgtccggcaa tacttgccag cacatagcct acggtccaca ccacgctgaa tgcgcccagc
    17401 aaaataccgc gatggcggcg cggggaaaat tcagccagca aggtgtgacc tactgaataa
    17461 tcgcctccca gaccaatgcc aatcaaaatg cgcagtccaa taagatgctc tggcgtggtg
    17521 gcaaaaaatt gtaaaaacga agcaagcgta atcagcaaaa agctgaaggt gaagattttt
    17581 tgccgaccaa tatggtcgga gatccaccca agaaccaggc tgccaaggaa caaaccaagg
    17641 agcgccgagc cgccgatcat tcccgccata aacggcgtca gttgcatggc gggcgtaagc
    17701 tgaataatgg cgtaaccaat gacgccgaga acataaccgt cggtcaggtg tgcgccgaaa
    17761 gtgagcgcag caatgcggca gtgaaaacgg ttaagcggta aatcatccat tcgcaccggt
    17821 gaagtgttca tctttacccc ttcattgtcc ggtaataaga acggcttctc gcctgagaag
    17881 ccgaaaaggc aacgccccgt catcgcggaa cgctgcctga cacattattt ttcaatcgga
    17941 tagatagtac ccgtattcat aatgccatta ggatcgaact gctttttcag cccttccagc
    18001 aacgcccacg cgctgccgtg ttccagtttg ctccagtgaa cgcgatgttt accgataccg
    18061 tggtggtgca ccatcgaacc gccgaggcga atggtttctt cacagatgat cttgttgagc
    18121 ggattgtggt acttgtcgat ttcctcttcc ggcttacagt caacgacgtt gtaatcgtag
    18181 acgaagtaca tgttggtgcc gttctgatag ctatgagagg aatgaccgcc cagcatggtg
    18241 atgtcgtcgg cgtgcgggaa ctcagtacga atacggttaa taacgctttc gtagatttcg
    18301 tggatgcagc tccagcagcc ggacacttca gtggtaaagc ccatgttgcc ggttttgagg
    18361 atctgcacac gttcggcagc cactttatcc ggtccccagt tcaggttgtt aaaccaggtt
    18421 tcgatcagtt tgctgtccac gcgctggcac tgcgggtagc gggcaacgat ttccgcaatc
    18481 ccttcgcccg tcgccttcgc aatgcgagga ttgccttcag ccataaagat cagcacacat
    18541 ttaccgtcgg caaaatgggt gaagtgttgg gtgccatctt cagcgtcata caaacgagcg
    18601 atcgacggac gatacccttc caccatgact tcacgcagga tgttgaagcc ggttttcatg
    18661 tcttccagga tatagccgta gaagaggttg ttttccgggg tgaatttaaa gattttcact
    18721 gttacttcag tgatatagca aagtgcacct tcgttaccga tgatgatgtg gcgaatgtcc
    18781 gggccagccg cgcggcgtgg cacgttttta atgcgtgtga cggtgccatc agccaatact
    18841 gcttccagac caacgaccat atcttcgatt
//
LOCUS       DAEVZA010000099        18701 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEVZA010000099 DAEVZA010000000
VERSION     DAEVZA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.099"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..344
                     /locus_tag="LAF92_003484"
     CDS             <1..344
                     /locus_tag="LAF92_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBI9792436.1"
                     /translation="VEQEVKALGVTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAP
                     VGTTLLEALESNNVPVVAACRAGVCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLAC
                     SCHPQGDLVLA"
     gene            477..2195
                     /gene="poxB"
                     /locus_tag="LAF92_003485"
     CDS             477..2195
                     /gene="poxB"
                     /locus_tag="LAF92_003485"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBI9792437.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            2232..3233
                     /gene="ltaE"
                     /locus_tag="LAF92_003486"
     CDS             2232..3233
                     /gene="ltaE"
                     /locus_tag="LAF92_003486"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBI9792438.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            3244..4674
                     /locus_tag="LAF92_003487"
     CDS             3244..4674
                     /locus_tag="LAF92_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBI9792439.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRVLMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            4773..5786
                     /locus_tag="LAF92_003488"
     CDS             4773..5786
                     /locus_tag="LAF92_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBI9792440.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(5783..6613)
                     /gene="amiD"
                     /locus_tag="LAF92_003489"
     CDS             complement(5783..6613)
                     /gene="amiD"
                     /locus_tag="LAF92_003489"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBI9792441.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(6610..6933)
                     /locus_tag="LAF92_003490"
     CDS             complement(6610..6933)
                     /locus_tag="LAF92_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBI9792442.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            7059..7574
                     /locus_tag="LAF92_003491"
     CDS             7059..7574
                     /locus_tag="LAF92_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI9792443.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            7792..8520
                     /gene="artP"
                     /locus_tag="LAF92_003492"
     CDS             7792..8520
                     /gene="artP"
                     /locus_tag="LAF92_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBI9792444.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            8538..9269
                     /gene="artJ"
                     /locus_tag="LAF92_003493"
     CDS             8538..9269
                     /gene="artJ"
                     /locus_tag="LAF92_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI9792445.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            9276..9992
                     /gene="artQ"
                     /locus_tag="LAF92_003494"
     CDS             9276..9992
                     /gene="artQ"
                     /locus_tag="LAF92_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBI9792446.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            9992..10660
                     /gene="artM"
                     /locus_tag="LAF92_003495"
     CDS             9992..10660
                     /gene="artM"
                     /locus_tag="LAF92_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBI9792447.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            10952..11683
                     /gene="artJ"
                     /locus_tag="LAF92_003496"
     CDS             10952..11683
                     /gene="artJ"
                     /locus_tag="LAF92_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBI9792448.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(11858..12985)
                     /gene="rlmC"
                     /locus_tag="LAF92_003497"
     CDS             complement(11858..12985)
                     /gene="rlmC"
                     /locus_tag="LAF92_003497"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBI9792449.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(13026..13514)
                     /locus_tag="LAF92_003498"
     CDS             complement(13026..13514)
                     /locus_tag="LAF92_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBI9792450.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(13574..14419)
                     /gene="potI"
                     /locus_tag="LAF92_003499"
     CDS             complement(13574..14419)
                     /gene="potI"
                     /locus_tag="LAF92_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBI9792451.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(14416..15369)
                     /gene="potH"
                     /locus_tag="LAF92_003500"
     CDS             complement(14416..15369)
                     /gene="potH"
                     /locus_tag="LAF92_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBI9792452.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(15379..16512)
                     /gene="potG"
                     /locus_tag="LAF92_003501"
     CDS             complement(15379..16512)
                     /gene="potG"
                     /locus_tag="LAF92_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBI9792453.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(16607..17719)
                     /gene="potF"
                     /locus_tag="LAF92_003502"
     CDS             complement(16607..17719)
                     /gene="potF"
                     /locus_tag="LAF92_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBI9792454.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(18070..18546)
                     /locus_tag="LAF92_003503"
     CDS             complement(18070..18546)
                     /locus_tag="LAF92_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBI9792455.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
BASE COUNT         4581 a         4989 c         4819 g         4312 t
ORIGIN      
        1 gggtagagca ggaagtgaaa gcgctcggcg tgacgcgttt ctttaaagag aaattcttca
       61 ccccagtagc agaagcggcg accagcggtc tgaaattcac caaactgcaa ccggcacgag
      121 aattttacgc cccggttggc accacgctac tggaggcgct ggaaagcaat aacgttccgg
      181 ttgtcgctgc ctgccgtgcg ggtgtttgcg gctgctgtaa gacaaaagtg gtttccggtg
      241 aatatacggt gagcagtaca atgacgctga ccgacgccga aatcgctgaa ggttacgtac
      301 tggcctgctc ctgccatccg cagggggatt tggttctcgc ataatcgcct tatgcccgat
      361 gatattcctt tcatcgggct atttaaccgt tagtgcctcc tttctctccc atcccttccc
      421 cctccgtcag atgaactaaa cttgttaccg ttatcacatt caggagatgg agaaccatga
      481 aacaaacggt tgcagcttat atcgccaaaa cactcgaatc ggcaggggtg aaacgcatct
      541 ggggagtcac aggcgactct ctgaacggtc ttagtgacag tcttaatcgc atgggcacca
      601 tcgagtggat gtccacccgc cacgaagaag tggcggcctt tgccgctggc gctgaagcac
      661 aacttagcgg agaactggcg gtctgcgccg gatcgtgcgg ccccggcaac ctgcacttaa
      721 tcaacggcct gttcgattgc caccgcaatc acgttccggt actggcgatt gccgctcata
      781 ttccctccag cgaaattggc agcggctatt tccaggaaac ccacccacaa gagctattcc
      841 gcgaatgtag tcactattgc gagctggttt ccagcccgga gcagatccca caagtactgg
      901 cgattgccat gcgcaaagcg gtgcttaacc gtggcgtttc ggttgtcgtg ttaccaggcg
      961 acgtggcgtt aaaacctgcg ccagaagggg caaccacgca ctggtatcat gcgccacaac
     1021 cagtcgtgac gccggaagaa gaagagttac gcaaactggc gcaactgctg cgttattcca
     1081 gcaatatcgc cctgatgtgt ggcagcggct gcgcgggggc gcataaagag ttagttgagt
     1141 ttgccgggaa aattaaagcg cctattgttc atgccctgcg cggtaaagaa catgtcgaat
     1201 acgataatcc gtatgatgtt ggaatgaccg ggttaatcgg cttctcgtca ggtttccata
     1261 ccatgatgaa cgccgacacg ttagtgctac tcggcacgca atttccctac cgcgccttct
     1321 acccgaccga tgccaaaatc attcagattg atatcaaccc agccagcatc ggcgctcaca
     1381 gcaaggtgga tatggcactg gtcggcgata tcaagtcgac tctgcgtgca ttgcttccat
     1441 tggtggaaga aaaagccgat cgcaagtttc tggataaagc gctggaagat taccgcgacg
     1501 cccgcaaagg gctggacgat ttagctaaac cgagcgagaa agccattcac ccgcaatatc
     1561 tggcgcagca aattagtcat tttgccgccg atgacgctat tttcacctgt gacgttggta
     1621 cgccaacggt gtgggcggca cgttatctaa aaatgaacgg caagcgtcgc ctgttaggtt
     1681 cgtttaacca cggttcgatg gctaacgcca tgccgcaggc gctgggtgcg caggcgacag
     1741 agccagaacg tcaggtggtc gccatgtgcg gcgatggcgg ttttagcatg ttgatgggcg
     1801 atttcctctc agtagtgcag atgaaactgc cggtgaaaat tatcgtcttt aataacagcg
     1861 tgctgggctt tgtggcgatg gagatgaagg ccggaggtta cctgacagac ggtactgagc
     1921 tgcacgacac caactttgcc cgcattgccg aagcgtgcgg cattacgggt atccgtgtag
     1981 aaaaagcctc tgaagttgat gaagccctgc aacgcgcctt ctctatcgac ggtccggtgt
     2041 tggtggatgt ggtggtcgcc aaagaagaat tagccattcc accgcagatc aaactcgaac
     2101 aggccaaagg ttttagcctg tatatgttgc gcgcaattat cagcggacgc ggcgatgaag
     2161 tgatcgaact ggcgaaaacg aactggctaa ggtaaaaagg gtggcatttc ccgtcataat
     2221 aaggacatgc catgattgat ttacgcagtg ataccgttac ccgaccgagc cgcgccatgc
     2281 tcgaagcgat gatggccgcc ccggttgggg acgacgttta cggagacgac cctaccgtta
     2341 atgctctgca ggactatgca gcagagcttt ccggtaaaga agccgccatt tttctgccta
     2401 ccggcactca ggccaacctg gtcgctctgc tcagtcactg cgaacgtggc gaagagtata
     2461 ttgtcggtca ggccgcgcat aactatctgt ttgaagccgg tggcgcagcg gtgctgggca
     2521 gtattcagcc gcaacccatc gacgcggctg ccgacggcac gctaccgctg gataaagtgg
     2581 cgatgaaaat caaacccgac gatatccatt tcgcccgcac caaattactc agtctggaaa
     2641 acacccacaa cggcaaagtg ctgccgcgtg aatacctgaa agaagcatgg gaatttaccc
     2701 gcgagcgcaa tctggcgctg catgttgacg gtgcgcgcat ctttaatgcc gtggtggctt
     2761 acggctgcga actgaaagag atcacgcaat attgtgattc gttcaccatt tgcctgtcga
     2821 aaggtcttgg gacgccagtc ggttcattac tcgtcggtaa tcgtgattac attaaacgtg
     2881 ccatccgctg gcggaaaatg gccggtggcg ggatgcgtca gtccgggatt ctggctgccg
     2941 ccgggatgta tgccctgaaa aataacgtcg cacggttgca ggaagatcac gacaacgccg
     3001 cctggatggc ggagcaactg cgtgaagcag gcgcggatgt gatgcgtcag gacactaata
     3061 tgctgtttgt tcgcgtcggg gaagaaaatg ctgccgcgtt aggcgaatac atgaaagcga
     3121 gaaacgtgct gattaacgcc tcgccgattg tccgcctggt gacgcatctt gacgtctcgc
     3181 gcgaacaact ggcggaagtc gccgcccact ggcgcgcatt cctggcgcgt taaggagaga
     3241 gacgtgccgc aacgcatttt agttctcggt gccagtggct acattggtca gcatctggtg
     3301 cgcacactca gccagcaagg gcatcagatc ctggcggcgg cacgtcatgt cgacaggctt
     3361 gcaaagctgc aactggcaaa cgtcagttgc cataaagtcg atctcagctg gccggataac
     3421 cttccggccc tgttgcagga tatcgatacc gtctattttc tggtgcacag catgggcgaa
     3481 ggcggcgatt ttatcgctca ggagcgccag gtggctctca acgtccgcga tgcgctacgt
     3541 gaagtaccag ttaagcaatt aatctttctc agttcgttgc aggccccgcc acatgagcag
     3601 tcggatcatc tgcgtgctcg tcaggctacg gcggacattc ttcgtgaagc gaatgtacct
     3661 gtgaccgaac ttcgggccgg aattatcgtt ggcgcaggtt cagcggcgtt cgaagtcatg
     3721 cgcgatatgg tctacaacct gccagtgtta acgccgccac gctgggtacg ttcacgcacc
     3781 acgcccatcg cgctggaaaa cttgctgcac tatctggtgg cgttgttaga tcatccggcc
     3841 agcgaacacc gcatcttcga agccgccgga ccagaggtgc tcagttatca gcaacagttt
     3901 gaacatttta tggcggtgag cggtaagcgc cgatggttga tccccatccc cctccccacc
     3961 cgctggattt cggtgtggtt tctcaatgtg attacttccg taccgcccac caccgccagg
     4021 gcgttgattc aggggctgaa acacgatctg ctggcggatg ataccgcgct acgtgcactc
     4081 atcccacaac ggctgatcgc tttcgatgac gcggtacgta gcacgttgaa agaggaggaa
     4141 aaactggtca actccagcga ctggggctac gacgctcagg cctttgcccg ctggcgaccg
     4201 gagtacggtt attttgccaa acaggcgggg tttaccgtta aaacgtccgc cagccttgct
     4261 gctttatggc aggtagtgaa ccaaatcggc ggtaaagagc gttatttctt tggcaatatt
     4321 ttgtggcaga cacgggtgtt gatggaccgc gcgatcggtc ataagctggc gaaaggccgc
     4381 ccggagcgcg aatatttaca gactggcgat gcggtagata gctggaaagt gattgtcgtt
     4441 gaaccggaaa aacaacttac gttgttattt ggcatgaaag cgccggggct gggacgactg
     4501 tgttttagcc tggaagataa aggcgactat cgtactatcg atgtccgcgc tttctggcat
     4561 ccgcacggta tgccggggct gttttactgg ttattgatga tccccgcgca tctgtttatt
     4621 tttcgcggaa tggcaaaaca aatcgccaga ctggcagaac aaagcacaga ttaatcgaca
     4681 aaacctgcgt tattctttca tgtttcccat tgcatcatgg gggaaatcac ggaagaatgc
     4741 gcacgttatt ttctcaacaa cagtggattg atatgaaggt actggttacc ggcgccacca
     4801 gcggcttagg tcgaaacgcg gtagagtttt tatgccagaa aggcatcagc gtgcgagcga
     4861 ccggtcgcaa cgaggcaatg ggcaaattgc tggaaaaaat gggcgcagag tttgttccgg
     4921 cggatctgac cgagctggtt tcatcacaag ctaaagtgat gctcgcgggc attgatacgc
     4981 tgtggcactg ctccagcttt acctcaccct gggggacaca acaggctttc gatctggcta
     5041 acgttcgcgc cactcgccgc ctgggtgaat gggctgtcgc ctggggtgtg cgtaacttta
     5101 ttcatatctc ttccccctcc ctgtacttcg attatcacca ccatcgcgat attaaagaag
     5161 attttcgccc tcaccgcttc gccaacgagt ttgcccgcag caaagcagcc agcgaagaag
     5221 tgatcaatat gctttcgcag gcgaatccac aaacgcgctt tactattctg cgcccacaaa
     5281 gtctgttcgg accgcacgat aaagtcttta ttccccgtct ggcgcatatg atgcaccact
     5341 acggcagtat tctgttaccg catggcggca gtgcgctggt cgatatgacc tactatgaaa
     5401 atgccgtgca cgcaatgtgg ctggcaagcc aggaagcctg cgataagcta ccttccgggc
     5461 gtgtgtacaa catcaccaac ggcgagcatc gcacactgcg cagcatcgtg cagaagctga
     5521 tcgacgagtt gaatattgac tgtcgtattc gttccgtccc ctacccgatg ctggatatga
     5581 tcgcccgcag catggagcgt ttaggccgca agtcagcaaa agagccgccg ctgacccact
     5641 acggcgtctc caagttgaat tttgacttta cgctggatat tacgcgtgcg caggaagagt
     5701 taggttatca gccggtcatc accctggatg aaggtatcga gaaaactgcc gcctggctac
     5761 gcgaccacgg aaaactgccg cgctaatcct gcccgtattt ctccagcaat gcttcggcaa
     5821 tcgcctgagt ttctgcatcc gcttcgccgt tatataacgt cgggcggaaa tgcatctgga
     5881 atgccataat cacgcgccgc tgctcgcgcg gtgtcatatc aggtttaacg tcataaccgt
     5941 agcgcgccaa aagctccagc aatgacgcgg tatctaccgg tgtgtgcggc gcgcgcccgg
     6001 caaggtaaaa gttaacccgc tgcgcatccg gccaggcacc aatcccctgc tgcgccagtt
     6061 gctgccaggg aaataatggc cccggatcgt ctttgcgctg cggtgcgata tccgcatgtg
     6121 ccactacgtt ttccggcttg atgtgataac gggcaataat atctttcgcc agtggaataa
     6181 gcgcctgaat ctgtgccggt tcaaacgggg caaaatattt cactccggcc gatttttgcc
     6241 agccacggtt ttccagctca atgccaatag aggtgtcgtt aaggcgcgtt gccccgcgcc
     6301 aggcgctaat ccccgcatgc caggccagtt cttgttccgg caccagttgc cagatgcgcg
     6361 gtttaccgtt gtatcgcggt ggtaccgcag ggaccagata atgcgagctg acctgcttat
     6421 cggtcagtgt cgccagcgag ctatcaaaat catctgcggt atagtggatc accagcactt
     6481 taatgcgcgg atacgccgcc tgcgcctggc gtcgggtatc aagctgatat ccctctttct
     6541 cgacaatgcc tttttcgcct gcacaccctg ccagtaacag agcggcagcg atcagccaga
     6601 agactcttct catcgacgcg ttttcaccgc cgtaccgctg acgctaacca tcagcatact
     6661 gccgttttgc ccgaccgttt cgtagtcgat atcaataccg acgacggcat cggcccccag
     6721 cgcccgcgcc tgggagccta attcctcaaa ggcgatctcc cgtgctttac gcagttcttt
     6781 ttcatacgca ccggaacgtc cgccaacgat atcgcggata ccggcaaaga aatcacggaa
     6841 aatattggca cctaaaatcg cttcgccggt caccacaccg caatattcaa cgatggtctg
     6901 gccttccaga gttggggttg tagaaaattg catgatcgat ctccttgttt gttaagcctt
     6961 cgatctctaa agcattatca gactgatacg ctattattga aaggatatca ttattatcgt
     7021 agaacgcttt cagagcgatc gcttataagg aaatcattat gcgctacagc aaattgacaa
     7081 tgcttatccc ctgcgcactg ctcctcagtg cctgcaccac agtcactcca gcttataaag
     7141 ataacggcac acgcagtggt ccttgcgttg aaggaggccc ggataacgtg gcccagcaat
     7201 tttatgacta ccgcattttg caccgcagca acgacatcac cgccttgcgc ccttatctga
     7261 gcgacaaact ggcgacactg cttagcgatg cctcccggga taataaccat cgggaactac
     7321 tgaccaacga tccattctcc agtcgcacaa ccttgccaga gagcgcccgt gttgccagtg
     7381 cctcaacaat cccgaatcgt gatgcgcgaa atattcctct gcgtgtggat ctaaaacagg
     7441 gtgatcagag ctggcaggat gaagtgttaa tgattcagga aggccagtgt tgggtgatcg
     7501 acgatgtacg ctatttaggc ggcagcgttc atgccacggc gggtacgctc cgtcagtcca
     7561 tcgagaatcg ctaacgcctt gatcaaccaa taaaccccgt aaattgtccg gcatttcgtg
     7621 aggaatgccg acatttatgc tcgccgacca ccgcccccgt tattttgtgc tatgtttatt
     7681 gaataatgcg ctttgctttt aacttttaaa gcagaaatat tgcataatta ttctgtcaaa
     7741 ggtactatct tcgacctcaa tagctatcag actgccagta tacgagtgtc aatgagtatt
     7801 caattaaacg gcattaattg cttctacggc gcgcatcagg cgctgttcga tatcacgctg
     7861 gattgcccac agggcgaaac gctggtgtta cttggcccca gcggcgcggg taaaagctcg
     7921 ctgctgcgtg tactcaatct gcttgagatg ccgcgctccg gtacgctcaa cattgcaggc
     7981 aaccatttcg atttcaccaa aacaccctca gacaaagcga ttcgcgattt gcgtcgtaac
     8041 gttggcatgg tgtttcagca atacaacctg tggccgcatc tgaccgtgca gcaaaacctg
     8101 attgaagcgc cctgccgtgt actggggttg agtaaagatc aggcactggc ccgtgcagaa
     8161 aaactgctgg aacgtctgcg tctcaaacct tatagcgatc gttacccgct gcatctttct
     8221 ggtggtcagc agcagcgtgt tgctattgcc cgtgcgttga tgatggaacc gcaggtactg
     8281 ctgttcgatg aaccgaccgc cgcactggac ccggaaatta cggcacaaat cgtcagcatc
     8341 attcgtgagc tggcagaaac gaatattacc caggtgatcg tcacccacga agttgaagtg
     8401 gcgcgtaaaa ccgccagccg agtggtgtat atggaaaatg gtcatatcgt agaacaaggc
     8461 gacgcgagct gctttaccga gccgcaaacc gaagcattta aaaactatct ctctcactaa
     8521 gattcgggat aacaacaatg aaaaaagttc tgattgccgc gttaattgca ggttttagtc
     8581 tttccgccac agctgccgaa accattcgtt ttgctaccga agcctcctat cctccgtttg
     8641 aatcgattga tgcaaacaac cagatcgttg gttttgacgt cgacctggca caggcactgt
     8701 gtaaagagat tgatgcaacc tgtactttct ctaaccaggc gtttgacagc ctgatcccaa
     8761 gcctgaaatt ccgtcgcgta gaagcagtga tggcgggcat ggatatcact ccggagcgtg
     8821 aaaagcaggt actgtttacc accccgtact atgacaactc tgccctgttt gtgggtcagc
     8881 aaggcaaata caccagtgtt gatcagctga aaggcaaaaa agtcggcgta cagaacggga
     8941 cgacacacca gaaattcatt atggataagc acccggaaat cactaccgtt ccgtatgaca
     9001 gctaccagaa cgcaaaactg gatctgcaaa acgggcgtat cgacggcgtc ttcggtgaca
     9061 ccgcagtggt cactgagtgg ctgaaagata acccgaaact ggcggcggtg ggcgacaaag
     9121 tgaccgataa agattacttc ggcactggcc tcggcatcgc ggtacgtcag ggcaacactg
     9181 agctgcagca gaaactcaac actgcgctgg aaaaagtgaa gaaagatggc acttacgaaa
     9241 ccatctacaa caaatggttc cagaagtaat tcctgatgaa tgaatttttt cctttagcaa
     9301 gcgccgccgg gatgaccgtc ggccttgccg tttgtgcatt gattgtcggg ctggcgctgg
     9361 cgatgttctt tgcggtatgg gagtcggcaa aatggcgtcc tgtcgcgtgg gcaggttcag
     9421 cgctggtaac cattctgcgt ggcctgccag aaattctggt ggtgctgttt atctattttg
     9481 gctcctcgca gctgctgctg acgctttcgg atggcttcac tatcaatctt gggttcgtgc
     9541 agatcccagt gcagatggac attgagaact tcgacgttag cccgttcctt tgtggtgtca
     9601 tcgctctgtc actgctgtat gccgcctatg cctcgcaaac gcttcggggc gcgttgaaag
     9661 cggtgccggt gggtcagtgg gagtctggtc aggcgctggg gctgtcgaaa tcggctatct
     9721 ttttccgtct ggtgatgccg cagatgtggc gtcatgcgct gcctggcctc ggtaaccagt
     9781 ggctggtgct gctgaaagat accgcgctgg tcagtttgat tagtgtgaat gatttaatgc
     9841 tgcaaacaaa aagcatcgct actcgtaccc aggaaccatt tacctggtac attgtggcgg
     9901 cggcgattta cctggtgatc accctgctca gtcagtacat tctcaaacgc attgacctgc
     9961 gcgcgacacg ttttgagcgg aggcccagct aatgtttgag tatttacccg aactgatgaa
    10021 agggctacac accagcctga cgctaaccgt tgcctcgctg attgtggcac tgattctggc
    10081 attgattttt accatcatcc tgacgctgaa aacgccggtg ctggtgtggc tggtgcgggg
    10141 ttatatcacg ctgtttaccg gtacgccgct gctggtgcag atcttcctga tttattacgg
    10201 gccgggccag tttccgactt tgcaggagta tccggcactg tggcatttgt tgtcagaacc
    10261 gtggttatgt gcgctgattg cgttatcgct gaacagtgcg gcgtatacca cgcagctgtt
    10321 ttacggtgca attcgtgcga tcccagaagg tcagtggcag tcctgtagcg ccctgggaat
    10381 gagcaaaaaa gatacgctgg cgatcctgct gccgtatgcc tttaaacgct cgctctcttc
    10441 ttattccaac gaagtggtgc tggtgttcaa aagtacctct ctggcttaca ccattacgct
    10501 gatggaagtg atgggataca gccagttgtt gtacggacgc acctacgatg taatggtgtt
    10561 cggtgcggca gggattattt acctggtcgt caacggcctg ctgacgctga tgatgcgtct
    10621 gatcgagcgc aaagcactgg cattcgaacg gcgaaattaa tgtagctgcg tacattcgcc
    10681 gggggggttt gtaggtctga taagacgcgc cagcgtcgca tcagacatca gcacggtgcc
    10741 tgtgccggat gcggcgtgga cgccttatcc ggcctacata gtgcataaat tcttataatg
    10801 aagacggaca actcactaag gtatccgtct ttttttattt catttaaatt atttaatcat
    10861 atttattgca tataaattca cttgatggca ttgttatccc atgccgcaga cacggccaaa
    10921 aatcataaga ttgacagacg ggagttccat catgaaaaag ttagttcttg ccgctttact
    10981 tgcttccttt actttcggtg cttctgccgc agagaaaatc aattttggcg tttcagccac
    11041 ctatccaccc tttgaatcta taggtgctaa taatgagatt gtcggctttg atatcgatct
    11101 ggcaaaagcc ttgtgcaaac aaatgcaggc agaatgtact tttactaatc acgcgttcga
    11161 cagcctgatc ccgtcactga aattcagaaa atatgacgcc gtaatctccg gtatggatat
    11221 cacgcccgag cgtagcaagc aggtatcgtt taccacgccc tactatgaaa actcagccgt
    11281 cgtgattgcc aaaaaagata cctacaaaac gtttgccgat ctgaaaggca aacgtattgg
    11341 gatggaaaac ggcaccacgc accagaaata tattcaggat cagcaccccg aagtgaaaac
    11401 tgtctcctat gacagttatc agaatgcctt tatcgatctg aaaaatggtc gtattgatgg
    11461 ggtatttggt gacacagcgg tggtaaacga atggctgaaa accaatccgc aactgggcgt
    11521 tgctactgaa aaagtgaccg atccgcaata cttcggcacc ggtctgggca tcgctgtacg
    11581 tccggataac aaagctctgc tggaaaaact gaataacgcg ctggcagcaa ttaaagctga
    11641 cggtacatat cagaaaatca atgaccagtg gtttccgcag taattgagac tgatgacaaa
    11701 agtaagattg cctgatgcgc tgcgcttatc tggcccacat caactctgca atgtattgat
    11761 ttagcatgat tttgtaggcc ggataaggcg tacacgccgc atccggcatg aacaaagcgc
    11821 acctgatcag caatctgaag ccgcacctgc ggctttttta ttgcttcacc agcagcgtca
    11881 tcacttcata gtgcgcggtg tgcgggaaca tatcaaaaag ctgtacccgt tcgatgcggt
    11941 aacctggcag ttcgcggata tctttcgcca tggtttgggc gttacagctg gagtagataa
    12001 taaaacgcgg tgccatcgtt gagagataat cacacagcgg tttaccaatg ccgcggcgcg
    12061 gcgggttaac cagcaccagc tccggcacat ccccctgagc ggtggcaaac tgagtggagt
    12121 ccagcgcctg aaattgcaga cgcgttaaac ccagttcagc ggctgactgc tttgcacagg
    12181 caatggcctc tggcgcaatt tcgatcccgg ttaactgcat gtcaggcgtc gcgcagtgta
    12241 aaccaaagcc ccccacaccg cagaacagat cccacatatg tttaaccggc agctgtcgta
    12301 cccagtcgcg cgcggtggcg tacaactggc tggcgaccgc cggattcgtc tggaagaaac
    12361 tttgcggacg gatccacagc ggtacgtcat taaaacgctc tgccagtgcc tgttgttcgg
    12421 tcaggtagat ctccgtctcc ccttccataa tcgccatatg taccggctga atattgacgg
    12481 taataacttt cagctgcggt agttgttcgt gtaaccacgg cagcgcctta cgcagttgcg
    12541 ccagcttggt atcagaacgc agtacaaagc gcagcatcat gccgccatca ctctggcttt
    12601 cagtcagcag aatgtatttc agttcgccac gcttacgcgc cacgttgtag ggcgttaacc
    12661 ccgctcgggc gataaacggt tttagcgccg caaaaacggg cgcaaatgag gcaggataaa
    12721 gcgggcagtc acaaaggtct tctggtgtgc cgtcgcgatg cagcataccg agcagtggtt
    12781 tttcaacgct accactcacc accattttgg ctttattacg aaacccttgt tccgggcctg
    12841 acaccggcgc gcaccattcc tcaaccggaa aatcggcgag cagattttta agatcggcgg
    12901 ttttagcgga gagttgctct ggaatcggct gcgttatcca ctgacaggaa cgacagcgac
    12961 ccgcgtcgta aagtgcgcac tgcatacatt gaccttcaca tcatcagggg cgacgattat
    13021 acacattatt gcaactggaa gaaccgccga ctggtcgagg gaacgaacag cagcatcagg
    13081 atgagcatat ccggcagctt ctgcagcatc aggctatgga agatttcacg ttttgattca
    13141 ccgggaatgc tgaacagctc cggataaccg taccccagcg aagccgccca caagtaactt
    13201 gcggcggtga tttgtgtcag cagatatagc cagcgcgccc agcgacgccc tttcaccagt
    13261 gaaaacgcac accagatctc aatgaaaacc agcaccagac tgcttaaaaa gaccagcgtt
    13321 aaactccagg tttgtacgct gcgatgaatg aactcgccaa taccgcgcac gcccagcgta
    13381 ttgaaaatca tcagcacgtc gaggccacgg atcataataa tggcgagcgc cgccacctgc
    13441 accagcgcag gcacattcag gcgagcatga gatgaagatg ttttcttaaa aaatcccaac
    13501 gtttcgtctt ccatgaaaac aatgccgcgg catgcgcggc attatgtagc caggttggca
    13561 aattttagtg tcttcagcca tgtcttgcac gctggatatc gcgtatccgc tgtttttctg
    13621 cgcgagccat cagataccag gcgataaatc cgacaattcc gaccgcaccg agaattaacg
    13681 ttgcaagggc gttgatttcc ggattcaccc ccatccgcac gctggaaaag accagcatcg
    13741 gtaacgtggt ggctcccggc ccggaaacaa agctggcgat caccagatca tcaagcgaca
    13801 aagtaaaagc cagtaaccag ccagaaatga tcgcgggcat gatcatcggt agcgtaatga
    13861 cgaaaaacac tttcagcggc gtcgcaccga gatccatcgc tgcttcttct atcgagcgat
    13921 ccagttcccg caatcttgaa gaaataacga ccgccacata agccgtacaa aaagtgacat
    13981 gcgccagcca gatggtgagc ataccgcggt ccgcaggcca gccaatggca tgagcaagcg
    14041 cgacgaataa taacaacagc gacaagcccg tgatgacatc tggcatcacc agcggcgcgg
    14101 tgatcataaa ggcaaagcca tttgatccgc gaaacctgcc aaagcgcacc agcaccaccg
    14161 ccgcaatagt cccgaggatc gccgccgccg ttgccgcaca ggcagcaatt gtcaggctta
    14221 aaccaaccgc actcatcatc gcgtcatcgc gcaataactc accataccag cgcgttgacc
    14281 agccggccca caccgtcacc agcttcgagc tgttaaacga atagataacc agcatcagca
    14341 ttggcgcgta gagaaaggta aagcccagca gcaaaatcac aatccgccag ggcgaacgaa
    14401 ctaccggtaa attattcatc cgtgttctcc cacgcttttt tgctggtgtt tgtggaacca
    14461 cattatcggc acaattagca gcagcaacat gatgatcgct accgccgagg ccaccggcca
    14521 gtcgcggtta ttgaaaaact cttgccatag cacgcgcccg atcatgatgc tgtccgggcc
    14581 accgagcagt tccgggatca caaactcgcc cacagccggg ataaacacca gcatcgatcc
    14641 ggcaataatc ccacctttag tcagcggcac gatcacagta aagaacgttt tcagcggtcg
    14701 tgcaccaaga tccagcgctg cttccaccag cgaataatca atacgaatca acgcggtata
    14761 aatcggcagt accataaacg gcacgtaggc gtaaacaatg ccaatataaa ctgccagatt
    14821 ggtatgaaga atggtcagcg gttgatcgat aacccccagc cacagcagaa aattattcag
    14881 cacaccgttg ttttttaata ttcccatcca ggcataaacg cggatcagaa acgaggtcca
    14941 cgacggcagg atcaccagta gtaataaaat attacgggtc gaaggcttac tgtgcgccac
    15001 cgcccacgcc agcggatagc cgatcagtaa acagcaaaat gtcgaaatcg cagccacctg
    15061 tagcgactgg agataagcat cgaaatagag cggatcgtcg gtcagttgca gaaaattacc
    15121 gagattaaga gtgatggaca gttgcccgtc agcccactcc atcagttcgg tataaggtgg
    15181 aatagcgcgc gccatctccg ccaggcttat tttaaagaca atcagaaatg gcagcagaaa
    15241 cagcaagatc aaccagatat atggcaacgc aatgaccagt ttgcgcccat gcttcatttg
    15301 cagctgcgac aaccacagct taaatccgcc cggcggtttc gactgggcag caggttcaag
    15361 tgtattcatc tttgctcctt aaaccgtcag caccacacag ctgtccactt cccagcacaa
    15421 acgcacttcg tcgccccagg tcggtaaccc tttacgatga cgatgggcgt tttgtagctg
    15481 ggcgctaatc atctgcccac ttttcagacg aacgtgatac accgaaagat cgccgagata
    15541 ggcaatgtgt atcacctccc ccaccgcgaa gttacaacca ttggcgggcg gctcttcgca
    15601 aagcatgatt ttttccgggc gcagcgctac gtgtaccggc acgttatcga ccactgaagc
    15661 atccgcatcg actttcagtg gatgcaccag ccccggcgaa taaagcacca ggccatcttc
    15721 ctgacgctct ttgagtacac cttcaaagac attaaccgaa ccgataaatt cagcgctgta
    15781 gcgggtagtt ggatgctcgt agatctcttc cggctcgcca atctggacaa atttcccgcg
    15841 attcataatg gcgatacgcc ccgccatggt catcgcctct tcctgatcgt gggtgaccat
    15901 cacacaagtc acaccgacgc gctccagaat atccaccact tcaagctgca tccggtcacg
    15961 cagcttttta tccagcgcgc ccatcggctc atcgagcagt aatagtttcg ggcgtttggc
    16021 aaggcttcgg gccagggcca cacgctgtcg ctgaccaccg gaaagctgat gcggtttgcg
    16081 tttggcgaac tcctgcatgt gcaccaggcc gagcatctca tggacccgac tggcaatttc
    16141 cgctttcggt agtttgtcct gtttcaggcc aaaagcgatg ttctgttcca cggtcatatg
    16201 cggaaacagc gcgtaagact gaaacatcat attgatgggg cgcaggtaag gcggcacctg
    16261 tgacaaatcg acgccatcaa gcattatctg tccggcagaa ggttgttcga aacctgccag
    16321 catacgcagc agcgtggact tgccacagcc ggatgcgccc agcagcgcga agatttcacc
    16381 tttgtagatg gtcaggctga catcatccac cgcatgttga ccatcgtagg atttggtgag
    16441 gttgcggatt tctaatagcg gcgtcagcgc cttacgggtt ttcgcctgcg ggcgagggat
    16501 agcgtcattc acggctgctc tccggcattg aatgtgtgca agcgcaccat gatggtgcga
    16561 attgccggcg ggtgtggtgc gcccgcccgg catctaaggc tgcggattat tttccgctct
    16621 tcactttggt ccacgcgcgg gtgcgcacac ggtcgatttt cgggtcctgc actttcagag
    16681 tgaacagctt cgcacgaaca tccgcaggcg gataaatacc cgggttctca cggacttccg
    16741 cactcaccag cggcgtggct gctttattgg cgttggcata gaacacatgg tcggaaatat
    16801 gcgctactac atccgggcgc agcaggtaat tcaggaactg ataggcttcg tctttgtttt
    16861 tggcatccgc aggcatggcg aatacatcaa agaacgccat cgccccttct tttggaatcg
    16921 agaacgagac attcactcca ttcttcgctt ccttcgcgcg gtttgacgcc tgccagacat
    16981 cacctgccca gccgatagcg acgcaaatat cgccgtttgc caggtcgtta atgtattgag
    17041 atgaatggaa ataacgaatg ttcgggcgca gctttaacag cagatcggtt gccggtccgg
    17101 tgtaatcatc cgctttagtg ctgttgggat ctttgccgag ataattcaac acggtagcaa
    17161 aaacttcttc tggcgcatcc aggaaagaga caccgcagct tttcagtttt tccagatttt
    17221 caggtttgag gatcaagtcc cagctatcga cgggcgcgtt ttcgcccagc acagctttaa
    17281 ctttatcaac gttatagcca atcccggtcg tcgcccacat atagggcata gcaaatttat
    17341 tgtcgggatc gtgtttggcg accagcttca gcagctccgg gtcgagattc ttccactccg
    17401 gcaatttgct tttgtccagc ggctggaaaa ctcccgcagt caactggcgt tcaagaaagc
    17461 tggcagatgg aaccaccaag tcaaagccgg tgctacccgc cattaatttg ccttccagta
    17521 cttcgttaga gtcgaaaaca tcgtagacga ctttaatacc ggtttctttt tcaaaattgg
    17581 ccaccgtgtc cggggcgata taatcagacc agttataaac gtggagtgtt ttttgttcag
    17641 ccgcgagcgt gccgacagag acggccatca gagcacccgc agccagaccc gaaagccatt
    17701 ttttatttaa ggcggtcata tctctttcct tctgaaagtt cgttaacaaa cgaacaaaag
    17761 ttgtcacact aaaagatatg caataaacgt gcatattttt tatcggcggg gtgtaagaaa
    17821 agagaactct ctcccggcca gcactgctcg ctatataaaa gcatcgcaag aataacttta
    17881 gccagggttt gagactatag ctcggattaa aaaacggtat ttaacaaatt tttatgcgta
    17941 tttgctctat gtgataaagc caaatcgaca caagagaagt gaaataatct ttaagaaaag
    18001 gttaaaaaca gcagataatt ttgcagcgcg cagccactgc accagcgact gcgtgctcga
    18061 aaggagttat cagtggagga aatagttttc ggtaacgttg ttttgccctt catcgtcagt
    18121 cggtaacagc agttgatggg cattagcttc aagaatgacc atcgaaatct gctcttcgct
    18181 ctgacgcacg aaccatgcaa actgctcata ggttacgccc tgcataacgg ataaagactg
    18241 gcaaaccacc agctttggca gattatcatc ctgcatatca agaaatgctt tcacggtcag
    18301 cgaactggca ttgatggcag ataaatccgc cgccagcggc aatacggctg aaggtcggac
    18361 ttccgccatg gcagaaaaca ggatcgtgtt atcgatcaga tcgattttgg catcaaagac
    18421 accgtcgaaa ttctgcatat ggggcagatg cagagcctga cagttatcac attcaaaaaa
    18481 actcatcccc aggtcatcga gccattgacg cagcgtatcc agaccaggaa cgaccagcga
    18541 tgtcataatt ttgtacaacc tctttcgata aaaagaccgg cacagcttac gcaaaaagcg
    18601 caggcaaaac catgatcagt aatgtgattg cgactaacca cccgttttca ggcaatattc
    18661 tgtcgtagcg tggcgttcga tccagcggat cattttaccc g
//
LOCUS       DAEVZA010000100        18107 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEVZA010000100 DAEVZA010000000
VERSION     DAEVZA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.100"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1071..2636
                     /locus_tag="LAF92_003504"
     CDS             1071..2636
                     /locus_tag="LAF92_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBI9792456.1"
                     /translation="MEWIADPSIWAGLITLIVLELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLTSISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRRGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HDNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEKEIRELLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEAEEIDARHDIQKNADGSWTVNG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPQPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDG"
     gene            complement(3106..4959)
                     /gene="asmA"
                     /locus_tag="LAF92_003505"
     CDS             complement(3106..4959)
                     /gene="asmA"
                     /locus_tag="LAF92_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBI9792457.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAPGASQPLVRADNMRLDVALMPLLSHQLNVQ
                     QVMLKGAVIQLTPQTKEVRDENAPIAPRDNTLPDLSEDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIHLQMEQDPQHRGSFEFSGRVNRDQRDLSLSLKGTVDASDYPHDLTA
                     VVEQITWQVQGADLPKQGIQGQGSFLAQWLEASKRLSFNQINLTANDSTFGGQAQVTL
                     TEQPEWQLKLQFPQLNLDNLIALNETANNNNGAVQQGQNQSMLPRPVISSRIHEPPYQ
                     GLKGFSADILLQADNVRWRGMNFANVATQMTNKSGLLEITQLQGKLNGGFVSLPGTLD
                     ATSANPQITFQPRLENVEIGTILKAFNYPIPLTGKMSLAGDFSGADIDAEAFRHKWQG
                     QAHIEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLERFTTDLTLNNGIVT
                     LNDMQGQSPMLALTGAGTLNLTEQTCDTQFDIRVIGGWNGESKLIDFLKATPVPLRVY
                     GKWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKL"
     gene            complement(4981..5562)
                     /gene="dcd"
                     /locus_tag="LAF92_003506"
     CDS             complement(4981..5562)
                     /gene="dcd"
                     /locus_tag="LAF92_003506"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBI9792458.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLNEDEAFFLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5654..6295)
                     /gene="udk"
                     /locus_tag="LAF92_003507"
     CDS             complement(5654..6295)
                     /gene="udk"
                     /locus_tag="LAF92_003507"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBI9792459.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            6613..9672
                     /locus_tag="LAF92_003508"
                     /pseudo
     CDS             6613..9672
                     /locus_tag="LAF92_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9843..10631)
                     /gene="alkA"
                     /locus_tag="LAF92_003509"
     CDS             complement(9843..10631)
                     /gene="alkA"
                     /locus_tag="LAF92_003509"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBI9792460.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEH
                     RGVVTVVPDIDSHSLRLNLSTGLEPVATECLVRISRLFDLQCNPQMVNGALGELGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVAQLYGERLDDFPDYVCFPTSQR
                     LAVADPLALKALGMPLKRAEALIYLARAALEGSLPITTPDDIEQAMKRLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTSAQIRRYAERWRP"
     gene            10765..12117
                     /gene="yegD"
                     /locus_tag="LAF92_003510"
     CDS             10765..12117
                     /gene="yegD"
                     /locus_tag="LAF92_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI9792461.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHEVPANDDETQALLRRAIRYNREEDIDVTAKSVQFGLASLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQSQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQCDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSETKTCASLPFISNELTTHISQQDLDNALNQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPVEGGDDFGSVTAGLARWADVVFR
                     "
     gene            complement(12404..12460)
                     /locus_tag="LAF92_003511"
     CDS             complement(12404..12460)
                     /locus_tag="LAF92_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI9792462.1"
                     /translation="MMKLLIIVVLLVISLPAY"
     gene            12739..13986
                     /gene="mdtA"
                     /locus_tag="LAF92_003512"
     CDS             12739..13986
                     /gene="mdtA"
                     /locus_tag="LAF92_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBI9792463.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVLAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            13986..17108
                     /gene="mdtB"
                     /locus_tag="LAF92_003513"
     CDS             13986..17108
                     /gene="mdtB"
                     /locus_tag="LAF92_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBI9792464.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            17109..>18107
                     /gene="mdtC"
                     /locus_tag="LAF92_003514"
     CDS             17109..>18107
                     /gene="mdtC"
                     /locus_tag="LAF92_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBI9792465.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEE"
BASE COUNT         4281 a         4834 c         4660 g         4332 t
ORIGIN      
        1 gattacctct gtcgatatcg acggcacttt gttcttttca ttcaccaaat gaacattttc
       61 cagcaacgat cgtgtttcgg cctttgccat aaaccagcca cagacgtcct cgcacagggc
      121 taagctcaca gttaatatcg gcgagcgaca cttatatata tcattgatat ttaataaaaa
      181 aataaatcac cacctgtcat attaccccag caaagcacac ttcatttcaa tgcgggtcaa
      241 cacgataaag gggagatttc ctgcgaagat ttagattctg cgtaattccc agacacggtt
      301 agcattatca agatgaatat tacctgatcc aaagttttgc agtatggaga ggtatttgct
      361 tttaatcgct ctgtagccga ttaacatctc cacacttttc atcacattaa tattatcaac
      421 agcaaaatta tcaatcccca ttttcctact ggaatctggc gtgatattgc aaatggtgga
      481 atgccagtca tcctcaacat ggagtagcgg tcagcagcca gatttgtgat attcgtcaca
      541 acaacgtttt atcctgccag aatgtatccc gttatccatt ccgcaagaaa cgctgggtgg
      601 tgattaaggt atttgaatat tgactctcca gtaatgcagg aggcagtgtt acaactaatg
      661 gattatcagg taaaaccatt agcgattttc aggaattata tgaagcatat ttcacgacgc
      721 tttttagtac actctttagg ctggtggctt ttaatacaac atccccttgt acaatatttg
      781 ttaaccagaa ttataatttt ctcacgtaca acaaatgatg gatattgtgc cgataagctc
      841 caggataatg tcggataaat ggtgctgtaa cattgcattt tggccctgat ttatccatga
      901 tcgaagggtg acatttatca ttactcatca taattcaagt tgcaggtgct gcggttgtgt
      961 tcgttttccc cggtcactga cttcagtaag ccccagggaa ttcactctct taccgccttc
     1021 ctgccactcg aattatgtag agtaaccaac gaataggtat tgtaattact atggaatgga
     1081 ttgccgatcc gtctatctgg gctgggttaa tcacgctgat tgtgctcgaa ctggtcctcg
     1141 gcattgataa cctggttttt attgccatcc tcgccgaaaa actcccgccg aaacagcgcg
     1201 accgcgcacg agtaaccggg ttactgttgg cgatgttaat gcgcctgtta ttgctgacgt
     1261 caatttcctg gctggtcacc ctgacccagc cactgtttag cttccgctcg tttaccttca
     1321 gcgcccgcga cttgattatg ctgttcggtg gtttcttcct gctgttcaaa gccactatgg
     1381 aactaaacga acggctggag ggaaaagaca gcaataaccc cactcagcga cgcggtgcga
     1441 aattctgggg ggtggtaacg cagatagtgg tgctggacgc cattttctca ctcgattcgg
     1501 tgattaccgc tgtcgggatg gtcgatcatt tattggtaat gatggctgcc gttgttatcg
     1561 ccatcagcct gatgctgatg gcaagcaaac cgttaacgca atttgttaat agccatccga
     1621 ccatcgttat tctctgcctg agcttcctgt tgatgatcgg ctttagcctg gtagcggaag
     1681 gtttcggttt cgtcattcca aaaggttatc tgtatgccgc aattggtttc tcggtgatga
     1741 tcgaagcact caaccagtta gctatcttta accgacgccg ttttctttct gctaaccaga
     1801 ccctgcgcca gcgaaccact gaagcggtga tgcgtctgtt aagcgggcaa aaagaggatg
     1861 cagaactgga tgcagagacc gcctcaatgc tggtagatca tgacaaccag caaattttta
     1921 atccacagga acggcggatg atcgagcgcg tgctcaatct gaaccagcgt accgtcagca
     1981 gcattatgac gtcgcgccac gatattgagc atattgatct caatgcgccg gaaaaagaga
     2041 tccgcgagtt gctggagcgg aaccagcaca cgcgcctggt ggttacagac ggtgacgatg
     2101 cggaagattt gctgggtgtt gttcacgtta tcgacctgct gcaacagtcg ctgcgcggcg
     2161 aaccgctcaa cctgcgggta ctgattcgcc agccgctggt gttcccggaa acattgccgt
     2221 tattgcctgc cctggagcag ttccgtaatg cccgtaccca ctttgctttt gtggtggatg
     2281 agtttggttc ggtggagggg attgtgacac tcagtgacgt caccgagacc atcgccggga
     2341 acttaccgaa cgaagcagaa gaaattgacg cgcggcatga tatacagaaa aacgcggacg
     2401 gttcctggac cgtgaatggt catatgccgc tggaagatct ggtgcaatat gtaccgctgc
     2461 cgttggatga aaaacgggaa tatcacacta ttgccgggct attgatggag tatttacagc
     2521 gtattccgca accaggcgaa gaagttcagg taggggatta tttgctcaaa acgttgcagg
     2581 ttgaaagtca tcgcgtgcag aaggtgcaga ttattccgct gcgcaaggat ggctagatgg
     2641 agtacgaggt gtgatggtat cggtcatatt acccgatgct gcgtaacgcc ttataacttc
     2701 agcaatgaag cattgtctgt ggtaaacgga catcacagcg ggggtgttgg tccggctgta
     2761 aatcgccgaa tcgtttcctg taggtcgggg cagcccacat ggatgtgggc tgagggcgcg
     2821 ttttacaggg atgttacctc gcgcccgtac ccaatagccg aaaggaataa aatgagggga
     2881 ccgcgccagc ggcgatttac cgccgggagc ccgggttgcc agggtggtgg cgattgagcc
     2941 cccctggcac gttcaccggc ttgagcgttc catagtagcg aggaacataa agtgaacgga
     3001 acagccttcg atcttacata ttcaaaatat actggtgccg ccacgaacgc ctgatgcaac
     3061 gcttttcgcg ttttatcagg cctacaaatt atgtcatctg gaaacttaca gcttttccag
     3121 caacttcttc acatctttgc cattgcgcga atctttattc cgttcagccc aatcattcag
     3181 gcggcgtttg gcctcatcct gtaagtgttt gcgcaacagc tgatcaactt gcaggctgta
     3241 gttaagttgc tgccatttgc cgtatacccg cagtggaacc ggcgtggctt tcaggaaatc
     3301 aatcagctta ctttcgccat tccagccgcc aatgacgcga atatcaaact gggtatcgca
     3361 agtctgctct gtcagattca acgttcccgc ccctgtcagc gccagcattg gcgattggcc
     3421 ctgcatgtcg tttaacgtca caatgccgtt attaagcgtc agatcggtag taaaacgctc
     3481 aaggcgtgtc acgttatcga agttttcagc cgctttcaca tcaccgccgt tgcgttctac
     3541 cgcctgctgg atcatctgct ggaagttcat cccttccatg cgtgtatcgg ccatttcaat
     3601 atgcgcctgc ccttgccact tatggcggaa cgcttcggca tcaatgtctg cgccagagaa
     3661 atcacccgcc agcgacattt ttccggtcag cggaatagga tagttgaacg ccttcaggat
     3721 agtgccaatc tcaacgtttt ccagtcgcgg ctgaaaggtt atttgcggat tcgcagacgt
     3781 cgcatccagt gttcccggca acgaaacgaa tccaccgttg agtttgcctt gcagttgggt
     3841 aatttccagc aagcccgatt tgttggtcat ttgcgtggcg acattggcaa aattcatccc
     3901 acgccagcgc acgttatcgg cctgtaacaa aatatctgcg ctaaaccctt ttaacccctg
     3961 ataaggcggt tcatgaatgc gcgaagaaat gaccggacgc ggcaacattg actgattttg
     4021 cccttgctga acagcgccat tgttattgtt cgcagtttca ttaagcgcaa tgaggttatc
     4081 aagattcaac tgcggaaatt gcagttttag ctgccattcc ggttgctctg tcagagtcac
     4141 ctgtgcttgc ccaccaaacg tactgtcgtt ggcggtcaaa ttaatctgat taaacgacag
     4201 gcgcttagag gcttccagcc attgcgccag gaaacttccc tgcccctgaa tgccctgttt
     4261 cggtaagtcc gcaccctgca cttgccaggt gatttgctcc actaccgccg ttaaatcatg
     4321 cggataatca gaagcatcca ccgtaccttt gagcgacagg ctaagatcgc gctgatcgcg
     4381 attaacgcgc ccggagaact caaacgaacc acgatgttgt ggatcctgct ccatttgcag
     4441 atggatatta cgaatcgtaa cctgttcgtc atcctcatgc tggaatacca gcacgctgtc
     4501 ggcaaccttc aggctggaaa tatcaaacga ccaaccgcga tcttcagaca gatccggcaa
     4561 agtattgtcc cgcggcgcaa tgggcgcgtt ttcatcgcgc acctctttcg tctgcggcgt
     4621 cagttggatc accgcccctt tcagcatgac ctgctgaacg ttcagttgat gactcagcag
     4681 cggcataagc gccacatcaa ggcgcatatt atcagcacga accagcggct ggctggcgcc
     4741 aggggcagtg agggacattc gcccagagag gatactcagt tgcggccaga cgtgccaacg
     4801 cagcggcccg tccaactgca attgataacc gctacgtgca gcgacctgct tcaccatata
     4861 gtcgcggaaa tcattcggat tcaccagcaa caccagcgca gataacccgg caaccagtac
     4921 gaccaggagt atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcag tggacctgaa
     4981 ttagtctttg tcgattcggc tggcgaccgc gccctgctgg ttgcgatatt tcgcatcttc
     5041 acggcggttg taaggacgcg cagccgggcc ggaaagcggc tcaaagctca gcgcgccaat
     5101 taacatgcca gggcgtagcg ccagcggcaa tttaccagag ttataaaact ccagcacaat
     5161 gcagccagac cagccgggat cgatacggtg cgctgtcacg tgaaccatta gtcccagacg
     5221 cgccagtgaa gaacgcccgt ccagccagcc aaccagatcg gctggcaatg ttaccgactc
     5281 cagcgtaacc gccaatgcca gttcacctgg atgcaggaaa aatgcctcat cctcattgag
     5341 cacgatttca tcgctcatca cgcgatcaag cgcagcgctc acttcgtcct ttggaccgct
     5401 cagatcaata aatgccgccg tatggccacg gaaggtacga aatttattgc ccaaccggac
     5461 atccaccgtt gcgccattaa tacgctccac aggtggacgt gggttgatcg acaaacggcc
     5521 ttcatcaagc caggcttcaa tatctcggtc acacagacgc atggcatttt ctcctttcgc
     5581 gcatcacgcc ctcaacgcca gttacgtcaa gggcgtacca ggttatcact gaacggtaca
     5641 taattcacaa aacttattca aagaactgac taattttcgc tttcaatata tcgatggcga
     5701 tacggttttt accaccgcgc ggtacgataa tgtccgcata ttgtttggaa ggttcaatga
     5761 attgcaggaa catcggacgc acggtttttt gatattgcgc cattacggaa tccatcgaac
     5821 gaccgcgctc gttaacatca cgtttaatac gacgcatcag gcagatatcc agcggcgtat
     5881 caacaaaaat cgagaagttc agttcgtcac gcagacgcgc atccgtcagc aacaaaatgc
     5941 cttcgagaat aatgactttc ttcggttcaa ccgtcaccgt ttctttcata cgcgtatgtt
     6001 caacgtagct gtaaaccggc aagtcaatag ccgagccgcg tttcagcgcc tgcaaatgct
     6061 caagcagcag gctgtgatcc atcgcgctgg gatggtcgta gttagtctta acacgttctt
     6121 ccatcgacag atggctttga tctttgtaat aacagtcttc gggaattacg ccgatgtgtt
     6181 catcgcccac ttgttcacgt aattcacgat aaagggtact ggcaataaga ctcttgccgg
     6241 aagccgatgc gccagcgata ccgataatga cgcactgatg agactgatca gtcatatatt
     6301 tagcgacctg attaacctgg atgttaggaa gggggcgcct aagcgccaaa cgcggcaatt
     6361 atagggattt catccgcgtg ataccagtcg aatagcgctg acgccatttt cccccaattg
     6421 ctggcaatcc attccctgac gcaaatttaa ttgtttgcct ttaacgcaaa gtaaaaaatg
     6481 ttatgcagta ttgcgcaaat tatccgttac acactcactt tatgaacaat taacatatta
     6541 aatgtaaaac ttttgtacta gcataaactc agagaacaat actgatgacc tggccatgcg
     6601 gatagagcag taatgagcaa acaatcacag catgtattaa ttgccctgcc ccatcctctg
     6661 cttcacctcg tcagtttagg tttagtctcg tttattttta ctctcttctc gctcgaactg
     6721 tcgcagtttg gtacgcaact tgcgccattg tggtttccca cgtccattat gatggtggcg
     6781 ttttatcgtc atgccggacg catgtggccg gggatcgcgc tgacctgctc gctggggaac
     6841 atcgccgcct ccgtactgct tttttccacc agctcactga acatgacctg gacgataatc
     6901 aacattgttg aagccgtggt tggcgcaatg ctgctgcgga aattgttacc gtggcataac
     6961 ccattgcaaa atctggctga ctggctgcgc ctcgcattgg gtagcgccgt cgtgccgcct
     7021 gtattaggcg gtgtgctggt cgttttactg aggcctggcg atgctccgct aaaagcgttt
     7081 ttaatctggg tactttcaga atccatcggc gcactggcgt tagtgccgct gggactgctg
     7141 tttaaaccac attacctgtt acgccatcgc aatccccggc tgttgtttga aacgctattg
     7201 acgctagcca tcacgctgac attaagctgg ctttcgatgc tgtacctgcc gtggcctttt
     7261 actttcatca tcgtcttgct gatgtggagc gctgtgcgtt tgccgcggat ggaagctttt
     7321 ttgatattcc tcaccacggt gatgatggta tcgctgatgc tggctgcaga ccctttactg
     7381 ctcaccacgc cgcgcaacaa cgtaatgaac aatatgccat ggttgccatt tttgctgatc
     7441 ctcttaccgg ccaacatcat gactatggtg atgtacgcct ttcgcgctga acgcaaacat
     7501 atttcagaaa gcgaaacgcg ttttcgcaac gccatggaat attccgccat tggcatggcg
     7561 ttagttggcg ccgagggtca atggctgcaa gctaataaag cgttatgcca gtttctcggt
     7621 tacagccagg aagagctacg cgccctcact tttcagcaac tgacctggcc ggaagatctg
     7681 aataaagatc tacaacacgt tgaacagttg gttaatggtg aaattaacac ctactcgatg
     7741 gagaagcgct attacaccca caatggcgac gttgtctggg cgctgcttgc ggtctcatta
     7801 gtgcgccacg ccgatggcac gccactctac tttatcgccc agattgaaga tattaacgat
     7861 ctcaaacgca ccgagcaggt caataaacaa cttatggagc gcatcacgct ggctaacgag
     7921 gcgggcggaa ttgggatttg ggagtgggaa ctcaagccca atatatttag ttgggataaa
     7981 cggatgttcg aactgtatga aattccgccg catatcaaac caaactggca ggtgtggtac
     8041 gactgcgtgt tgccggaaga tcgcgaacac gccgaaaaag tgattcgcga ttcattgcag
     8101 gcgcgcttgc cctttaagct ggaatttcgc atcgccgtga aagacggtat tcgtcatatc
     8161 cgctccctcg ctaatcgggt actgaataaa gaaggcgagg ttgaacatct gctcggcatt
     8221 aatatggata tgaccgaagt taaacaactg aatgaagcac tgttccagga aaaggagcgg
     8281 ctacacatta cgctggattc catcggtgaa gcagttgtct gtattgatct ggcaatgaag
     8341 atcaccttta tgaatccgat cgccgagaag atgagtggat gggcgcaaga agaggcgctg
     8401 ggggttccgt tgctgacagt gttacatatt acctttggcg acaatggtcc actgatggag
     8461 aatatctaca gtgcagatac ctcgcgttca gctattgaac aagatgtcgt gcttcactgc
     8521 cgtaatggcg gcagctataa cgtgcattac agcattacgc cgttaagcac tcttgatggc
     8581 agcaatattg gttcggtact ggtgattcag gatgtgaccg aatcacgcaa aatgctgcgc
     8641 cagctgagtt atagcgcctc ccatgatgct ctgactcacc tggctaaccg caccagtttc
     8701 gagaaacaac tgcgaacgtt gctgcaaacg gtaaacagta ctcatcagcg gcacgcactg
     8761 gtgtttatcg accttgatca ctttaaagcg gtgaatgaca gcgccgggca cgcggcaggc
     8821 gatgccctgc tgcgcgaact ggcatcgcta atgttaagca tgctgcgctc cagtgactta
     8881 ctggcacgac tcggcggcga tgaatttggc ttactgttgc cggactgcaa catcgaaagc
     8941 gcccgtttta tcgccacgcg cattattggt gcggtaaatg actaccactt tatatgggaa
     9001 ggacatgtac accgggtcgg cgccagtgcc ggaattacgc tgattgatgg ctgtaatcat
     9061 caggccgcag aagtgatgtc gcaggcagat atcgcctgtt acgcctcaaa aaatggcggc
     9121 cggggtcggg taaccgttta cgaaccacag caagccacgg cacatagcga gcgggcagtc
     9181 atgccgcctg atgaacaatg gctgatgatt aaagaaaatc aactcatgat gttcgctcac
     9241 ggcgttgctt cgccacggat cccggaaaca tgtaatttgt ggctgatttc acttaagctc
     9301 cagaacgacg aaggcaatgt tattgatgaa cacgtttttc gtcacggctt cagcgatccg
     9361 gcgctcagtc acgcccttga ccgcaggata ttgcatgaat ttttccagca aacggcaaaa
     9421 tcggctgccg ataaaggatt aagcatcgcc ctcccctttt ccgttgccgg cttaagcagc
     9481 gttacattgg tagacgaatt gcttgagcaa ctggaaaaaa gcccgctacc gccgcggttg
     9541 ttgcatttga ttattcctgc agaagccatc atcgatcacg cacaggccat tcaaaaacta
     9601 cgactggcgg actgccgtat tgtgctcagt caggtgggac gtgatctaca aattttcaat
     9661 acgctgaaag caatattgcc aatactcatt gccggatgcg acgctgacgc gtcttatccg
     9721 gcctacaaat tgctcattcc ccagaacgga taaggcgctc gcgccgcatc cggcgaccag
     9781 cgtcatgctt cgtctggttg ccagccctcc gtataccaga tatgcaatag cgcataagaa
     9841 cgctagggtc tccagcgttc ggcataacgg cggatttgtg cagacgtcat tcccggaaat
     9901 cgctgtttaa tcaaataatc atctggcaaa aaaacgtctt tcgcctgcca gccgcgtaag
     9961 gcgaaataat ttgccgtcca gcgtccgata cctggaaaag tttgtagcct tttcatcgct
    10021 tgctcaatgt cgtcaggcgt cgttatcggt aaggagccct ccagcgccgc cctggccaga
    10081 taaatcaatg cttctgcccg tttcaacggc atccctagcg ctttcagcgc cagcggatcg
    10141 gctaccgcca gccgttgtga tgtcggaaag cagacataat caggaaaatc atccagccgt
    10201 tcgccatata actgcgccac tttggcggtc agttttgccg ccatcgccac actcaccagt
    10261 tggcctaaaa tggcgcgcac gccctgttca aaagcatcaa cgcttccggg taaacgcaaa
    10321 ccaggtcgcg ccgcacctaa ctcgcccaac gcgccgttaa ccatctgcgg attacattgc
    10381 aagtcaaaca aacggctgat tctcaccaga cactcggttg caaccggttc taatcccgta
    10441 ctcaagttca atcgtagtga atgactgtcg atatctggca caaccgtcac cacgcctcga
    10501 tgttctccca ccgccaggct gcgggcataa taattgtcag caaccgtttc cacaccgctc
    10561 actgcacgag cagcgagaaa tcccaacatc cacgaccagt cataaggcgg ttgccagttc
    10621 agggtataca tcgcatctcc ttgttcatcc gctttcagca taaacgttat ccagacgcta
    10681 cgctttgctt tcatattccg gttgtcgcga cagcaacatt tcgctaaagt cacgcccctt
    10741 cttcaccggc atggggatta tttcgtgttt attggttttg attacggtac agcaaactgt
    10801 tcagtggcgg taatgcgcga tggcaaacca cagttgctga aaatggaaaa cgacagcacg
    10861 ctgctgcctt caatgctttg tgcgccaaca cgtgaagcgg tcagcgagtg gttgtaccgc
    10921 catcatgagg ttccggcaaa cgacgatgaa acgcaggcgc tcttgcgtcg ggcgattcgt
    10981 tataatcgcg aagaagatat cgatgtcacg gcgaaaagcg tgcagtttgg tctcgcttca
    11041 ctggcgcaat atattgatga tccagaagaa gtatggtttg taaaatcacc gaaatcattc
    11101 ctcggagcca gcggattaaa accacagcaa gtggcgctat ttgaagattt agtctgcgca
    11161 atgatgttgc atattcgcca gcaggcgcag tcgcaactac cggaagcgat tacccaggca
    11221 gtaattggtc gtccgatcaa cttccaggga ctgggcggcg atgaagcaaa cgcccaggcg
    11281 caggggattc tggagcgtgc agcaaaacgc gccggattcc gcgacgttgt tttccagtat
    11341 gaaccggtgg cggctggact ggattacgaa gcaacgttgc aggaagaaaa acgcgtgctg
    11401 gtggtggata tcggcggtgg tacaactgac tgttcattgc tgctgatggg gccgcagtgg
    11461 cgttcgcgtc tcgatcgtga agccagtctg cttgggcaca gtggttgccg tatcggcggt
    11521 aacgatctgg atatcgcgct ggcatttaaa aacttgatgc cgctgctcgg catgggcggc
    11581 gaaaccgaaa aaggtatcgc tctgccgatt ctgccgtggt ggaatgcggt ggcgattaac
    11641 gatgtgcccg cccagtgtga tttctacagt agtgctaatg gtcgcctgct taacgatctg
    11701 gtgcgcgacg cccgcgagcc ggaaaaagtc gctctgttgc aaaaagtctg gcgccagcgg
    11761 ttaagttatc gactggtgcg cagcgcggaa gagtgcaaaa tcgccctttc cagcgaaaca
    11821 aaaacctgtg catcactgcc atttatcagc aacgaactga caacacatat tagccaacaa
    11881 gatctggata acgcgctcaa ccaaccgctg gcgcgcattc tggaacaggt gcaactggcg
    11941 ctggataacg cccaggaaaa accagatgtc atctatctga cgggaggtag cgcccgttcg
    12001 ccgctgataa aaaaagcact ggcagaacaa ctgccgggca ttccggttga aggcggcgac
    12061 gactttggct ccgttactgc cggtctggcg cgttgggcgg atgtggtctt tcgctaaagg
    12121 agacgtggct caacagcagc ttaaatctga ctgcacctgg gccgttttct cgaccgactg
    12181 tcgtaacagc gccatcaacc atatcaatac aaagacagcg aggctccggg ttactggcaa
    12241 aatctcaggt ggcaaacgaa gaaactttca gaattatccc ccgcagcata gataattttt
    12301 ctattcgcct cgcaagaacg aggaattgca gattgacagt aaggggagag tgacataatt
    12361 tcaggagtga gggttgagcg gggtttcccc cgccctggta gtcttagtaa gccggtaagc
    12421 taatcactaa gagcaccacg atgatgagta gcttcatcat gaccctttcc ttatttaagg
    12481 ccccttcctc gggaggggcc ttcccgtttc agcgtcccgc tgaaatcatc ggcttacctc
    12541 ctgtcgccat gtatgcagtc caccgctgac ttgcggataa aataatttcc tgtgtcattt
    12601 ctggatacgt gctcgcaaag tgtgccgcca ttcagacgat tcccgacagt gtttcataat
    12661 tcctccattt ttctccctta ttggctggct acactagtat cattccgcga aacgtttcag
    12721 gaagagaaac tcttaacgat gaaaggcagt tataaatccc gttgggtaat cgtaatcgtg
    12781 gtggttatcg ccgccatcgc cgcattctgg ttctggcaag gccgcaatga ctcccagagt
    12841 gcagccccag gggcgacgaa acaagcgcag caatcgccag cgggtggtcg ccgtggtatg
    12901 cgttccggcc cattagcccc ggttcaggcg gcgaccgccg tagaacaggc agttccgcgt
    12961 tacctcaccg ggcttggcac cattaccgct gctaacaccg ttacggtgcg cagccgcgta
    13021 gatggtcagt tgatggcgtt acatttccag gaaggccagc aggtcaaagc aggcgattta
    13081 ctggcagaaa ttgaccccag ccagttcaaa gttgcattag cacaagccca gggccaactg
    13141 gcaaaagata aagccacgct tgccaacgcc cgccgtgacc tggcgcgtta tcaacaactg
    13201 gtaaaaacca atctcgtatc tcgtcaggaa ctggatgccc aacaagcgct ggtcagtgaa
    13261 accgaaggca ccattaaggc tgatgaagca agcgtcgcca gcgcacagct gcaactcgac
    13321 tggagccgca tcaccgcacc agtcgatggt cgcgttggtc tcaagcaggt tgatgttggt
    13381 aaccaaatct ccagtggtga taccaccgga attgtggtga tcacccagac gcatcctatc
    13441 gatttgctct ttaccctgcc ggaaagcgat atcgctaccg ttgtgctggc gcaaaaagcc
    13501 ggaaaaccgc tggtggtaga agcctgggat cgcaccaact cgaagaaatt aagtgaaggc
    13561 acgctgttaa gtctcgataa ccaaatcgat gccactaccg gtacgattaa agtgaaagca
    13621 cgctttaata atcaggatga tgcgctgttt cccaatcagt ttgttaacgc gcgcatgtta
    13681 gtcgacaccg aacaaaacgc cgtagtgatc ccaacagccg ccctgcaaat gggcaatgaa
    13741 ggccattttg tctgggtgct gaatagcgaa aacaaggtca gcaaacatct ggtgacgccg
    13801 ggcattcagg acagtcagaa agtggtgatc cgtgcaggta tttctgcggg cgatcgcgtg
    13861 gtgacagacg gcattgatcg cctgaccgaa ggggcgaaag tggaagtggt ggaagcccag
    13921 agcgccacta ctccggaaga gaaagccacc agccgcgaat acgcgaaaaa aggagcacgc
    13981 tcctgatgca ggtgttaccc ccgagcagca caggcggccc gtcgcgcctg tttattatgc
    14041 gtcctgtggc caccacgctg ctgatggtgg cgatcttact cgccgggatt atcggttatc
    14101 gcgccctgcc cgtttcggcg ctgccggaag tggactatcc gaccattcag gtggtcacgc
    14161 tctacccagg tgccagcccg gatgtcatga cctctgccgt taccgcgccg ctagaacgcc
    14221 agttcgggca gatgtctggc ctgaaacaga tgtcgtcgca aagttccggc ggtgcgtcag
    14281 ttatcacttt gcagttccag ctaacattac cgctcgatgt cgccgagcag gaagtgcagg
    14341 ccgcgattaa cgctgcgacc aacttgttgc cgagcgatct gcctaacccg ccggtttaca
    14401 gcaaagtgaa cccggcagat ccgccgatca tgacgctcgc cgtcacctca accgccatgc
    14461 cgatgacgca agtggaagat atggtggaaa cccgcgtcgc gcagaaaatc tcgcagattt
    14521 ccggcgtcgg cctggtgacg ctttccggcg gtcagcgtcc ggctgttcgc gtcaaactta
    14581 acgctcaggc gattgccgcc ctcggcctga ccagcgaaac cgtgcgcacc gccattaccg
    14641 gcgctaacgt taactcggca aaaggtagcc tcgacggccc ttcccgtgcg gtcacgcttt
    14701 ccgcgaacga ccagatgcaa tccgccgaag agtatcgcca gctaatcatc gcctaccaga
    14761 acggcgcgcc aattcgtctg ggcgatgtcg caactgtaga gcaaggtgca gaaaacagct
    14821 ggctcggcgc gtgggcgaac aaagaacagg ccattgtgat gaatgttcag cgccagcccg
    14881 gtgctaacat tatctccacc gccgacagca ttcggcagat gctgccacag ctcactgaga
    14941 gtctgccgaa atcggtgaag gtgacagtgc tttccgatcg caccaccaat atccgcgcat
    15001 ccgtcgatga tactcagttt gaattgatga tggctatcgc gctggtagtc atgattatct
    15061 acctgttttt gcgcaacatt ccggcgacca tcattcccgg tgttgctgta ccgctgtcgt
    15121 taatcggcac tttcgcggtt atggtgtttc tcgatttttc aatcaataac ctgacactga
    15181 tggcgttaac tatcgccacc ggattcgtgg tcgatgacgc catcgtggtg atcgaaaaca
    15241 tttcccgcta tatcgaaaaa ggcgaaaaac cgttggcggc ggcgctcaag ggcgcaggtg
    15301 aaatcggctt taccattatc tcgctgacct tctcactgat tgcggtgttg atcccactgc
    15361 tgtttatggg cgatatcgtc gggcgactgt tccgcgaatt tgctattacc ctggcggtag
    15421 cgattttgat ctcagcggtg gtgtcgctga ccctgacacc gatgatgtgc gcgcggatgc
    15481 tcagccagga gtcgttgcgt aaacagaacc gcttctcccg tgcctcggaa aaaatgttcg
    15541 acaggataat cgccgcctat ggtcgtggac tggcgaaagt gctgaatcat ccgtggctga
    15601 ccttaagcgt ggcactcagc acgctgctgc ttagcgtgct gctgtgggtg ttcattccga
    15661 aaggtttctt cccggtacag gacaatggca ttattcaggg cactttgcag gcaccgcaat
    15721 ccagctcctt tgccaatatg gcccagcgac aacgccaggt cgcggacgtg attttgcagg
    15781 atccggcagt gcaaagcctg acctcatttg ttggcgttga tggcactaac ccgtcgctga
    15841 acagtgcacg tttacaaatc aacctcaaac cgttggatga acgtgatgat cgggtgcaaa
    15901 aagtcatcgc ccgtctgcaa acggcggtag ataaagtgcc gggcgtcgat ctcttcctgc
    15961 aaccaacgca ggatctgact attgatactc aggtcagccg cacccagtac cagtttacct
    16021 tgcaggccac gtcactggat gcgctcagta cctgggtgcc acagttgatg gaaaaactcc
    16081 agcaactgcc acagctttct gatgtctcca gcgactggca ggacaaaggg ctggtggcgt
    16141 atgtcaatgt tgatcgcgac agcgccagcc gtctggggat cagcatggcg gatgtcgata
    16201 acgccctgta caacgcgttt ggtcagcggc tgatttccac tatttatact caggccaacc
    16261 agtatcgcgt ggtgctggag cacaacaccg aaaatacccc aggcctcgcg gcgctggata
    16321 ccattcgcct gaccagcagc gacggcggcg tggtgccgct aagctcaatt gccaaaattg
    16381 agcagcgttt tgcgccgctc tccatcaacc atctggatca gttcccggta acgaccatct
    16441 cctttaacgt gccggataac tattcgctgg gcgatgcggt gcaggcgatt atggacaccg
    16501 aaaagacgct gaatctgccg gtggatatca ccacgcagtt ccagggcagc accctcgcct
    16561 tccagtcggc gctgggcagc actgtctggc tgattgtcgc ggcggtggtg gcgatgtata
    16621 tcgtgctcgg cattctgtac gagagcttta ttcacccgat caccattctc tcgacgctac
    16681 ccaccgcagg ggttggcgca ctgctggcgt tgctgattgc tggtagcgaa ctggatgtga
    16741 ttgcgattat cggcattatt ttgctgatcg gtatcgtgaa gaagaacgcc atcatgatga
    16801 tcgacttcgc gctggctgcc gagcgcgagc aaggcatgtc gccgcgcgag gcaatctacc
    16861 aggcttgtct gttgcgtttt cgtccgatcc tgatgaccac cctggcggct ctgcttggcg
    16921 cgctgccgct gatgttgagt accggggtcg gcgcggaact gcgtcgtccg ttaggtatcg
    16981 gcatggtcgg cggcctgatt gtcagccagg tgctgacgct gttcaccacg ccggtgattt
    17041 atctgctgtt cgaccgcctg gcattgtgga ccaaaagccg ctttgcccgt catgaagagg
    17101 aggcgtaagt gaagtttttt gccctcttca tttaccgtcc ggtggcgacg attttactgt
    17161 cggttgccat taccctgtgc ggcatactgg gcttccgtat gctgccggtc gccccgctgc
    17221 cgcaggtcga ttttccggtg attatggtca gcgcctcgct gcccggtgcg tcaccagaaa
    17281 caatggcgtc ttccgttgcc acgccgctgg agcgctcact tgggcgcatt gccggagtca
    17341 gtgaaatgac ctccagcagt tcgctcggca gcacgcgtat tattttgcag tttgattttg
    17401 accgggatat caacggcgca gcgcgtgatg tgcaggcggc gatcaacgct gcacaaagtt
    17461 tgctgcccag tgggatgccc agccgcccga cctatcgcaa agcgaacccg tcggatgcgc
    17521 caattatgat cctcacgctg acgtccgata cttattcgca gggtgaactg tacgatttcg
    17581 cctcgacgca gctggctccg acgatttcgc aaatcgacgg tgttggtgat gtcgatgtcg
    17641 gaggcagctc actgcccgcc gtacgcgtcg ggctgaatcc gcaggcgctg tttaatcagg
    17701 gcgtgtcgct ggacgacgta cgcaccgccg tcagcaatgc caacgtgcgt aaaccgcagg
    17761 gcgcgctgga agatggcact caccgctggc agatccagac caatgatgag ctaaaaaccg
    17821 ccgctgaata tcagccgttg attattcact acaacaacgg cggcgcggtt cgtctgggcg
    17881 atgtggcgac ggtgaccgac tcagtgcagg atgtgcgcaa cgccgggatg accaacgcca
    17941 aaccggctat tttactgatg atccgcaaac tgccggaagc caatattatc cagacggttg
    18001 acagcatccg ggcaaaatta ccggagttgc aggaaaccat tccggcggcg attgatctgc
    18061 aaattgccca ggatcgctcc cccaccattc gcgcctcgct ggaagaa
//
LOCUS       DAEVZA010000101        17793 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEVZA010000101 DAEVZA010000000
VERSION     DAEVZA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.101"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..449
                     /locus_tag="LAF92_003515"
     CDS             <1..449
                     /locus_tag="LAF92_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBI9792466.1"
                     /translation="VLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLIEVNNLA
                     ADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDETPVRVLD
                     EEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            446..1183
                     /locus_tag="LAF92_003516"
     CDS             446..1183
                     /locus_tag="LAF92_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBI9792467.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1183..3279
                     /locus_tag="LAF92_003517"
     CDS             1183..3279
                     /locus_tag="LAF92_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBI9792468.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3326..3604)
                     /locus_tag="LAF92_003518"
     CDS             complement(3326..3604)
                     /locus_tag="LAF92_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792469.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKTDLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            3874..4284
                     /gene="psiE"
                     /locus_tag="LAF92_003519"
     CDS             3874..4284
                     /gene="psiE"
                     /locus_tag="LAF92_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBI9792470.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4328..5803)
                     /gene="xylE"
                     /locus_tag="LAF92_003520"
     CDS             complement(4328..5803)
                     /gene="xylE"
                     /locus_tag="LAF92_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBI9792471.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6175..7065)
                     /gene="malG"
                     /locus_tag="LAF92_003521"
     CDS             complement(6175..7065)
                     /gene="malG"
                     /locus_tag="LAF92_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBI9792472.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7080..8624)
                     /gene="malF"
                     /locus_tag="LAF92_003522"
     CDS             complement(7080..8624)
                     /gene="malF"
                     /locus_tag="LAF92_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBI9792473.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(8778..9968)
                     /gene="malE"
                     /locus_tag="LAF92_003523"
     CDS             complement(8778..9968)
                     /gene="malE"
                     /locus_tag="LAF92_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBI9792474.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10333..11448
                     /gene="malK"
                     /locus_tag="LAF92_003524"
     CDS             10333..11448
                     /gene="malK"
                     /locus_tag="LAF92_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBI9792475.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            11520..12860
                     /gene="lamB"
                     /locus_tag="LAF92_003525"
     CDS             11520..12860
                     /gene="lamB"
                     /locus_tag="LAF92_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBI9792476.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13012..13932
                     /gene="malM"
                     /locus_tag="LAF92_003526"
     CDS             13012..13932
                     /gene="malM"
                     /locus_tag="LAF92_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBI9792477.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14161..15741
                     /locus_tag="LAF92_003527"
                     /pseudo
     CDS             14161..15741
                     /locus_tag="LAF92_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            15964..16461
                     /gene="ubiC"
                     /locus_tag="LAF92_003528"
     CDS             15964..16461
                     /gene="ubiC"
                     /locus_tag="LAF92_003528"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBI9792478.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            16474..17346
                     /gene="ubiA"
                     /locus_tag="LAF92_003529"
     CDS             16474..17346
                     /gene="ubiA"
                     /locus_tag="LAF92_003529"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBI9792479.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(17501..>17793)
                     /locus_tag="LAF92_003530"
     CDS             complement(17501..>17793)
                     /locus_tag="LAF92_003530"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="PlsB; catalyzes the formation of 1-acyl-sn-glycerol
                     3-phosphate by transfering the acyl moiety from acyl-CoA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HBI9792480.1"
                     /translation="ITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAV
                     FSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLTIESATQGEG"
BASE COUNT         4489 a         4379 c         4582 g         4343 t
ORIGIN      
        1 tggtgctggc cttcgctgaa gacggacaac aaaaatgggt cactcaggat caagccactc
       61 ttgtcacaca acatggtcgt ctggtgaaga ctttgcttgg cggcgacaac ctgattgaag
      121 tgaataacct tgccgccgac ccgctgatta aacccgcaca aattgttgat ggtgcaacat
      181 ggacgcgcac gatgggctgg accgagtacc agcaggtacg ctacgccacc gcacgctcag
      241 tcttcaaatg ggatggcacc gataccgtca aggtcggcag cgatgaaacc ccggttcgcg
      301 tgctggacga agaagtctcc accgaccagg cgcgctggca taaccgctac tggatcgaca
      361 gcgaagggca aattcgccag tcggaacagt atctcggcgc ggattatttc ccggtgaaaa
      421 ccactctcat caaggcggca aaacaatgat taaacaaact attgtcgcat tgattttgag
      481 tgtgggggcg tcatcggtct tcgcggcggg aaccgtcaag gtattcagca atggcagcag
      541 tgaggccaaa acgctgacgg gcgcagagta tttaatcgat ctggtaggcc agccgcggct
      601 ggcaaacagt tggtggcctg gtgcggtgat tagcgaagag ctggcaacgg cggcagcatt
      661 gcggcagcag caggcgttgc tgacacggct ggcagaacag ggcgcagatt ccagcgccga
      721 cgatgccgct gcgattaacg ccttacgcca gcaaattcag gcgttgaaag tgacgggcag
      781 gcaaaaaatc aatcttgatc ccgatatcgt ccgcgttgcc gaacgcggta acccgccgtt
      841 gcagggcaac tacacgctgt gggtcggacc accgccgtcc acggtcacgt tgttcgggct
      901 tatcagccgt cctggcaagc agccattcac tccaggtcgc gacgtggcga gctatctctc
      961 tgaccagagc ctgctcagcg gtgcggatcg cagctacgcg tgggtggttt acccggacgg
     1021 acgcacgcaa aaagcgccgg tggcttactg gaacaagcgt cacgtagagc cgatgcccgg
     1081 cagcattatt tatgttggcc tcgcggactc cgtctggagt gagacgcctg atgcccttaa
     1141 cgccgacatt cttcagactc tgacgcagcg gataccccaa taatgaaaaa aagacatctg
     1201 cttagcttac tggcgctggg cattagcatg gcttgctacg gcgaaacata tcctgcgccc
     1261 attggcccgt cgcagtcgga tttcggtggc gtaggattat tacaaactcc caccgcgcgt
     1321 atggcacggg aaggggagtt gagtctgaac tatcgcgata acgatcagta ccgttattac
     1381 tcagcttcag tgcaactctt cccgtggctg gaaacaacgc tgcgttacac cgacgtgcgc
     1441 acccggcagt acagcagcgt cgaagcgttc tctggcgatc aaacgtataa agataaagcc
     1501 ttcgatctca aactgcgttt gtgggaagag agttactggc tgccgcaagt ggcggttggt
     1561 gcgcgggata tcggcggtac ggggctgttt gatgcggaat atcttgttgc cagcaaagcc
     1621 tgggggccgt tcgattttac gctcggcctg ggctggggat atctgggcac cagcggtaat
     1681 gtaaaaaatc cgctctgttc agccagtgat aaatattgct atcgcgataa cagctacaaa
     1741 caggcgggat ctatcgacgg tagccagatg ttccacggtc ctgcctcact gtttggcggc
     1801 gtggaatacc agacgccctg gcaaccgctg cgcctgaaac tggagtatga aggcaataat
     1861 tatcagcagg attttgccgg gaagctggag caaaaaagta agtttaacgt cggtgcgatt
     1921 tatcgcgtta ccgattgggc cgacgttaac cttagctatg aacgtggcaa cacctttatg
     1981 tttggcgtca cgctgcgcac caactttaac gatctgcgcc cgtcctacat cgataacgcc
     2041 cgcccgcaat atcaaccgca gccgcaggat gccattttgc agcattcggt ggtggcgaat
     2101 cagttaacgc tgttgaaata caatgccgga cttgccgatc cgcagatcca ggcgaaaggc
     2161 gatacgctgt atgttaccgg cgagcaggtg aaatatcgtg attcgcgcga agggatcatc
     2221 cgtgctaatc ggatcgtgat gaatgatctg ccggatggga tcaaaacgat ccgcgttacg
     2281 gaaaatcgcc ttaacatgcc gcaggtgacg acggaaaccg atgtcgccag cctgaaaaat
     2341 catctcgccg gagagccgtt gggccacgaa acgacgctgg cgcaaaaacg cgtcgagcca
     2401 gtggttccgc agtccaccga gcagggctgg tatatcgaca aatcacgctt tgatttccat
     2461 atcgatccgg tgctgaacca gtcggtcggt ggcccggaaa acttttacat gtatcagctg
     2521 ggcgtgatgg gaacggcaga tttgtggctg acagaccatc tactgaccac cggcagcctg
     2581 tttgcaaatc ttgccaacaa ctacgacaag tttaactaca ccaatccacc gcaggactcg
     2641 cacttaccgc gcgtgcgtac ccatgtgcgc gagtatgtgc agaacgatgt ctatgtgaat
     2701 aacctgcaag ccaactactt ccagcatctg ggcaacggct tctacggtca ggtctacggt
     2761 ggttatctcg aaaccatgtt tggcggtgcg ggggcagaag tgttgtatcg cccgctggac
     2821 agcaactggg cgtttggtct ggatgccaac tacgttaaac agcgcgactg gcgtagtgca
     2881 aaagatatga tgaaattcac cgactacagc gtgaaaaccg gacatctgac cgcgtactgg
     2941 acgccatctt tcgctcagga tgtgttagtg aaagcgagcg tcgggcagta tctggcgggc
     3001 gataaaggcg gcacgctgga gatcgccaaa cgctttgata gcggcgtggt ggttggcggc
     3061 tatgccacga tcaccaacgt ttcgaaagag gagtacggcg aaggggactt caccaaaggc
     3121 gtgtatgtct cggtaccgtt ggatctcttc tcgtctggcc cgacacgcag ccgtgcggcg
     3181 attggctgga cgccgctgac gcgtgacggt ggtcagcaac ttgggcgtaa gttccagttg
     3241 tatgacatga ccagcgaccg tagcgtcaat ttccgctaag tcatgggaaa ggtgccagtt
     3301 ttcgcaatca ctggcaccct cattcttaat ggcctgttga gccatcagga acgcagttca
     3361 cccccggttt gcgatctggt gtgtaaaggc gaaaacattc tgtcttcctg tccgtcgata
     3421 tcggttgcag atctggttgc ctgagcgact gggccatcat tgggctggag acattgcttt
     3481 gcggtaggtc ggttttatca gcagtaccga gcggaccagc ataagcagga agaacagaga
     3541 ctgataacat caaagcagca aaataaggct tcatttttac cacctttatc aggttacgtt
     3601 tcatttgttc cagaggaaca tggtcgattt ttcgcgcatt gctggtggct gggaatcacc
     3661 tgaatgggtg atttttgaat taccggcttt ggtgcggttt gtcttgccgg atgcgccgcc
     3721 aggcgcggct tatccggcct acgggtaggt atatccggct ttggtggagg cgcgctccaa
     3781 atccaggttg aacaaaacat acacaaaaaa tatagatctc cgtcacattt ttgcgttata
     3841 caggaagctc gccactgtga aggaggtact gctatgacgt cactctctcg tccgcgcgtg
     3901 gagtttatct ccaccatttt gcagaccgta ctcaatcttg gcctgttgtg cctcggcctg
     3961 attttggttg tcttcctcgg caaagaaacg gtgcatctgg cagatgtgct gttcgcgcca
     4021 gaacaaacca gcaaatatga gctggtagaa ggactggtgg tttactttct ctatttcgaa
     4081 tttatcgcgc tgattgtgaa atactttcag tccggttttc acttcccgct acgttacttt
     4141 gtctatattg ggatcaccgc cattgtgcgc ttgatcatcg tcgatcataa atcgccactg
     4201 gatgtgttga tctactcggc tgcgatcctg ttactggtga tcactctgtg gttgtgtaat
     4261 tcgaaacggc tgaagcggga gtaaaaagac agcacgccga actggcgcgg cgtgctggac
     4321 aggaagatta cagcgtagca gtttgttgtg ttttcttcgt ttccggttcc cagagggctt
     4381 ccagctcctc aagggtttta cctttggttt ccgggacaaa tttccacata aatagtgctg
     4441 ccagaacgcc catacacccg taaatccagt aggagaaacc gttgtggaaa tgggccacca
     4501 gccaggagtt tttgtccatc atcgggaagg tccaggagac gaagtagttc gccagccact
     4561 gggccgccac cgcgattgcc agcgctttac cacgaatggc attcgggaag atttccgaca
     4621 gcagaaccca gcataccgga ccccaggaca tggcaaaggc ggcaacatag aacagcatcg
     4681 acagtagcgc cacaataccc ggtgcctgag tgtaaaacgc ggtaccgagg ctaaacatac
     4741 cgattgccat tccgagcgcg ccgataattt gcagtggctt acgaccaaat ttatccaccg
     4801 tcataattgc cagaacggtg aaggtgaggt tgataactcc gacaataatg gtctgcaaca
     4861 gcgcgatatc cgtgctggcc cccagcgttt tgaacacttc cggcgcgtag tacaacacca
     4921 cattgatgcc gacaaattgc tggaagatgg agagcataac gccgattaca atcacgccca
     4981 cgccaaacat cagcagacga ccaccggttt tgcggccatg atccagggag tgtttaattt
     5041 cctgtactgc ctgagttgca agcgtgttgc ccataatttt gcgcaggata ctttccgcct
     5101 gttcttgctt gccgcgtgac atcagccagc gaggactttc tggcacggta tacagcagca
     5161 ttaagaacag cagtgcaggg atacattccg aggcaaacat ataacgccag ccgtcagtat
     5221 tcagccagct ggcatcaccg gaacgggcaa taaaatagtt tacgcagtaa actaaaagtt
     5281 gcccgaaaat aatcgcaaac tggttaaatg aaaccagttt tccgcgaata tgagctggag
     5341 ccagttccgc aatatacatt ggtgaaagca ttgaggctaa accaacgcca ataccgccaa
     5401 taatacgata aataacaaat tccgggacat aacctgccag ataaataggt actgtgttgt
     5461 ccgggtttat agaggtaaaa ccaagttctg gccaggcaga acctacacca gaaataaaaa
     5521 acaggacagc agcaatctta agtgaatcac gacggccgaa gcggttactg caataaccac
     5581 cgagggcacc gccgatgatg caaccaatca gagcgctggc tacgcaaaat cctaacaggg
     5641 agttggcagc ggattcactt aagttttgtg gagcaacaaa gacggtattg agtgactcaa
     5701 cagtaccgga aataacggcg gtgtcgtagc caaataataa accacctaat gtagcgacta
     5761 aggtaatcga aaatatataa ctggaattat actgggtatt cattcagacc tgccttagac
     5821 cattctgatc ttttatcagt gcattgatgt tggaattgtc aggcagttaa gacaattatc
     5881 attttttaca atgcgtttac gtaatgtctt ttctgtgatc ttaattgtga taattatcaa
     5941 aaattgatga aaacaatact attgccgtga ctcagagcac gaaagagaat tatcgtaagt
     6001 gggaaaacaa ataacgtaaa aataataagt tctatgatgg gaatattaac cggcgaacga
     6061 ttcagattgc agacgaaaga aaaaaaggcg ctccgtggag cgccgaataa tagtcacaag
     6121 ttgggataac gtaagttgag ggtgcagcgg cataacattg gcagaacaac atctttaacc
     6181 tttcacacca cctgccgtca ggccgttgac cagccagcgt tgagccagca agaagacgat
     6241 ggtgatcggt aatgcagaca tcacggcagc ggcggcaaag tcaccccaca ggtagttttg
     6301 cgggttgagg tattgctgca tccccacggc cagggtgtag ctgtttacgt cacgcagtaa
     6361 cagcgacgcg accggaactt cagtaatggc agcgataaac gacaggatga ataccaccgc
     6421 cagaatcggt actgacagcg gcaacaggac aaggcggaag gcctgccacg gtgtcgcacc
     6481 atccagcgcc gccgcttctt ccagcgaact gtcgatggtt tcgaaatagc ctttgatggt
     6541 ccagacatgc agcgcaatcc cacccagata cgcgaaaatt acgccgccgt gagtattcag
     6601 gccaatgaat ggaatgtact cacccagacg atcaaacaac gcatacaacg cgaccagtga
     6661 aagtactgcc gggaacatct ggaaaatcag cattcctttc agcagcgtcg ctttgcctgg
     6721 aaagcgcata cgggcgaaag cgtaggcgca ggtggtggag agcgccacaa tgccaatcgc
     6781 ggaaatcccg gcgaccttca ctgagttcca cagccacagc agtaccggaa acggcggcgg
     6841 tgtaacgcga ccatcagcct gttccacgct aaaacctaat gccagtttcc agtgatccca
     6901 ggagatttgc tccgggatca ggctgccggt cgcaaagttc ccctggcgca gtgagatagc
     6961 gacgaccatc agcagcggga acataatcgc cgcgataaaa agtagcagta gcaggtgagt
     7021 aataaacaaa cgtgcttttt gcgatttcgg ttggaccatt gccatttttg ttatctccct
     7081 taatcaaact tcattcgcgt ggctttcagg ttcactatcg ccagcgcacc caccagcagg
     7141 aagatcagcg tggcaattgc tgccgccaga ccgaagtcct gacccccgcc gccttcaaaa
     7201 gcgatgcggt aggtgtagtt aacaagcagg tcggtataac cggctggcgt ggtcgtgcca
     7261 agacgatccg ggccgccgtt ggttaacagt tgaatcagca cgaagttgtt aaagttaaag
     7321 gcgaagctgg cgatcatcag cggcgttagc ggtttaatca gcagcggcag cgtaatctta
     7381 aagaagttct ggaacggacc tgcgccatcc attgctgagg cttcatacaa atcgtccgga
     7441 atcgctttca gcaagcccat gcagaggatc atcatgtacg gataacccag ccaggtattg
     7501 acgataatca gcatcgttcg ggcggtggtc ggatcgctga accaggcggg cttcacgcca
     7561 aacagcgcgc tcaacatcat gttgatctca ccgaagctct ggttaaacaa ccctttgaaa
     7621 atcaagattg aaatgaacga tggcaccgcg tagggcagaa tcagcaggac gcgatagacc
     7681 gctttgccac gcaacgcttc ccactgcacc agacacgcca gaaccatgcc gaccgccacc
     7741 gttaaaaaga cagtgatcag cgagaacacc acggtccaga cgaaaatggc gaggaacggt
     7801 ttctgaatgc cttcgtcggt gaagacgcgg gtaaagtttt tccagccggt ggtcacggtg
     7861 taaccggggc ttagcttttc atcaccccag ttgccgtcgg cggtaatgga ctggtaaaag
     7921 ccaatttggt tattcggacg atatttcacg ccgctctgat tattcgtcaa cgtgccgtca
     7981 ccgtcgagtg tgtagagcgg ctgcgtgcca gaaaactggc gcagggagct catcatcact
     8041 ttgttgccat ctggcagaat ggcggtaatg tcactcagcg cctgacgatt ctgggtaatc
     8101 acgcgcagat tcgcgcgttc gccttcgggc tgggcggtcg tttctttcag ttgcagtttt
     8161 tgctcgccgc caaatttaaa cgcgtcggag aggtaatttt tgccggtttc gccgtcgctg
     8221 agggctagtt gccattcatc gcccgccggg taaagaccaa aattataggt tttgcctgct
     8281 tgccaggagc gatctagcaa cacttcctgc gcacgttcaa aagtcagctg gttagtgctg
     8341 ctgtagttgg tgaaggcaat ggcgatggtg cagaccagag ggaagaggac gaataatccc
     8401 attccagcca ttcccgggta aacatagcgc caggcgtagg ctttacgatt ggcgaaaata
     8461 tacagccccg ctgaactcaa tatcagcgtg gtaatggcga acaggtattc cccttgtgcg
     8521 tacattaaaa caacaaggta acccaccagc aggccgagca gacctaacac tgaccatttc
     8581 agcgcgtcgc tttgccacca atgtttcttt ttaatgacat ccatggggtt cttcctcatt
     8641 ccaggacgga taaggctttc acgccttatc cgacaacaac tgcctgatgc gacactgatg
     8701 cgtcttatca ggcctacatg cgtttcaatt tcgtaggccg gacaaggcgt tcacgccgca
     8761 tccggcattt cacagcatta cttggtgata cgagtctgcg cgtctttcag ggcttcatcg
     8821 acagtctgac gaccactggc ggcgttgatc accgcagtac gcacggcata ccagaaagcg
     8881 gacatctgcg ggatgttcgg catgatttca cctttctggg cgttttccat ggtggcggca
     8941 atacgtggat ctttcgccaa ctcttcctcg taagacttca gcgccacggc acccagcggt
     9001 ttgtctttat tgaccgcttc cagaccttcg tcagtcagca gatagttttc gaggaactct
     9061 ttcgccagct ctttgttcgg actggcggcg ttaatacctg cgctcagcac gccaacgaac
     9121 ggtttagatg gttgaccctt gaaggtcggc agtaccgtta caccataatt cactttgctg
     9181 gtgtcgatgt tggaccatgc ccacgggccg ttgatggtca tcgctgtttc gcctttatta
     9241 aaggcagctt ctgcgatgga gtaatcggtg tctgcattca tgtgtttgtt tttaatcagg
     9301 tcaaccagga aggtcagacc cgctttcgcg ccagcgttat ccacgcccac gtctttaatg
     9361 tcgtacttgc cgttttcata cttgaacgca taacccccgt cagcagcaat cagcggccag
     9421 gtgaagtacg gctcttgcag gttgaacatc agcgcgctct tacctttcgc tttcagttct
     9481 ttatccagcg ccgggatctc ttcccaggtt tttggcgggt tcggcagcag atctttgtta
     9541 taaatcagcg ataacgcttc aacagcgatc gggtaagcaa tcagcttgcc gttgtaacgt
     9601 acggcatccc aggtaaacgg atacagcttg tcctggaacg ctttgtccgg ggtgatttca
     9661 gccaacaggc cagattgagc gtagccacca aaacggtcgt gcgcccagaa gataatgtca
     9721 gggccatcgc cagttgccgc aacctgaggg aatttctctt ccagtttatc cggatgctca
     9781 acggtgactt taattccggt atctttctcg aatttcttac cgacttcagc gagaccgtta
     9841 tagcctttat cgccgttaat ccagattacc agtttacctt cttcgatttt ggcgagagcc
     9901 gaggcggaaa acatcatcgt cgttaatgcg gataatgcga ggatgcgtgc acctgttttt
     9961 attttcataa tctatggtcc ttgttggtga agtgctcgtg aaaacaccta aacggactct
    10021 agtttcttta tacggcaacc tctttccatc ctccttgccc ctacgcccca ccgttgcttt
    10081 gtgtgatctc tgttacagaa ttagcggtaa tgtggagatg cgcacataaa atcgccacga
    10141 tttttgcaag caacatcacg aaattcctta catgacctcg gtttagttca cagaagccgt
    10201 gttctcatcc tcccgcctcc tcccccataa aaaagccagg gggtggagga tttaagccat
    10261 ctcctgatga cgcatagtca gcccatcatg aatgttgctg tcgatgacag gttgttacaa
    10321 agggagaagg gcatggcgag cgtacagctg caaaatgtaa cgaaagcctg gggcgaggtc
    10381 gtggtatcga aagatatcaa tctcgatatc catgaaggtg aattcgtggt gtttgtcgga
    10441 ccgtctggct gcggtaaatc gactttactg cgcatgattg ccgggcttga gacgatcacc
    10501 agcggcgacc tgttcatcgg tgagaaacgg atgaatgaca ctccgccagc agaacgcggc
    10561 gttggtatgg tgtttcagtc ttacgcgctc tatccccacc tgtcagtagc agaaaacatg
    10621 tcatttggcc tgaaactggc tggcgcaaaa aaagaggtga ttaaccaacg cgttaaccag
    10681 gtggcggaag tgctgcaact ggcgcatttg ctggatcgca aaccgaaagc gctctctggc
    10741 ggtcagcgtc agcgtgtggc gattggccgt acgctggtgg ccgagccaag cgtatttttg
    10801 ctcgatgaac cgctctccaa cctcgatgct gcactgcgtg tgcaaatgcg tatcgaaatc
    10861 tcccgtctgc ataaacgcct gggccgcaca atgatttacg tcacccacga tcaggtcgaa
    10921 gcgatgacgc tggccgacaa aatcgtggtg ctggacgccg gtcgcgtggc gcaggttggg
    10981 aaaccgctgg agctgtacca ctatccggca gaccgttttg tcgccggatt tatcggttcg
    11041 ccaaagatga acttcctgcc ggtaaaagtg accgccaccg caatcgatca agtgcaggtg
    11101 gagctgccga tgccaaatcg tcagcaagtc tggctgccag ttgaaagccg tgatgtccag
    11161 gttggagcca atatgtcgct gggtattcgc ccggaacatc tactgccgag tgatatcgct
    11221 gacgtcatcc ttgagggtga agttcaggtc gtcgagcaac tcggcaacga aacccaaatc
    11281 catatccaga tcccttccat tcgtcaaaac ctggtgtacc gccagaacga cgtggtgttg
    11341 gtagaagaag gtgccacatt cgctatcggc ctgccgccag agcgttgcca tctgttccgt
    11401 gaggatggca ctgcatgtcg tcgactgcat aaggagccgg gcgtttaagc accccacaaa
    11461 acacacaaag cctgtcacag gtgatgtgaa aaaagaaaag caatgactca ggagatagaa
    11521 tgatgattac tctgcgcaaa cttcctctgg cggttgccgt cgcagcgggc gtaatgtctg
    11581 ctcaggcaat ggctgttgat ttccacggct atgcacgttc cggtattggc tggacaggta
    11641 gcggcggtga acaacagtgt ttccagacta ccggtgctca aagtaaatac cgtcttggca
    11701 acgaatgtga aacttatgct gaattaaaat tgggtcagga agtgtggaaa gagggcgata
    11761 agagcttcta tttcgacact aacgtggcct attccgtcgc gcaacagaat gactgggaag
    11821 ctaccgatcc ggccttccgt gaagcaaacg tgcagggtaa aaacctgatc gaatggctgc
    11881 caggttccac catctgggca ggtaagcgct tctaccaacg tcatgacgtt catatgatcg
    11941 acttctacta ctgggatatt tctggtcctg gtgccggtct ggaaaacatc gatgttggct
    12001 tcggtaaact ctctctggca gcaacccgct cctctgaagc tggtggttct tcctcttttg
    12061 ccagcaacaa tatttatgac tataccaacg aaaccgcgaa cgacgttttc gatgtgcgtt
    12121 tagcgcagat ggaaatcaac ccgggcggca cattagaact gggtgtcgac tacggtcgtg
    12181 ccaacctgcg tgataactat cgtctggttg atggcgcatc gaaagacggc tggttattca
    12241 ctgctgaaca tactcagagt gtcctgaagg gctttaacaa gtttgttgtt cagtacgcta
    12301 ctgactcgat gacctcgcag ggtaaaggtc tgtcgcaggg ttctggcgtc gcgtttgata
    12361 acgaaaaatt tgcctacaat atcaacaaca acggtcacat gctgcgtatc ctcgaccacg
    12421 gtgcgatctc catgggcgac aactgggaca tgatgtacgt gggtatgtac caggatatca
    12481 actgggataa cgacaacggc accaagtggt ggaccgttgg tattcgcccg atgtacaagt
    12541 ggacgccaat catgagcacc gtgatggaaa tcggctacga caacgtcgaa tcccagcgca
    12601 ccggcgacaa gaacaatcag tacaaaatta ccctcgcaca acaatggcag gctggcgaca
    12661 gcatctggtc acgcccggct attcgtgtct tcgcaaccta cgccaagtgg gatgagaaat
    12721 ggggttatga ctacaacggc gatagcaagg ttaacccgaa ctacggcaaa gccgttcctg
    12781 ctgatttcaa cggcggcagc ttcggtcgtg gcgacagcga cgagtggacc ttcggtgccc
    12841 agatggaaat ctggtggtaa tagcaaaacc tgggccggat aaggcgttta cgccgcatcc
    12901 ggcataaaaa caggttgtca ttatctgaaa ggggcgaaag cccctctgat tatcgggttt
    12961 agcgcgctat tgcctggcta ccgctgagct ccagattttg aggtgaaaac aatgaaaatg
    13021 aataaaagtc tcatcgccct ctgtttatca gcagggttac tggcaagcgc gcctggaatc
    13081 agccttgccg atgttaacta cgtaccgcaa aacaccagcg acgcgccagc cattccatct
    13141 gctgcgctgc aacaactcac ctggacaccg gtcgatcaat ctaaaaccca gaccacccaa
    13201 ctggcgaccg gcggccaaca actgaacgtt cccggcatca gtggtccggt tgctgcgtac
    13261 agcgtcccgg caaacattgg cgaactgacc ctgacgctga ccagcgaagt gaacaaacaa
    13321 accagcgttt ttgcgccgaa cgtgctgatt cttgatcaga acatgacccc atcagccttc
    13381 ttccccagca gttatttcac ctaccaggaa ccaggcgtga tgagtgcaga tcggctggaa
    13441 ggcgttatgc gcctgacacc ggcgttgggg cagcaaaaac tttatgttct ggtctttacc
    13501 acggaaaaag atctccagca gacgacccaa ctgctcgacc cggctaaagc ctatgccaag
    13561 ggcgtcggta actcgatccc ggatatcccc gatccggttg ctcgtcatac caccgatggc
    13621 ttactgaaac tgaaagtgaa aacgaactcc agctccagtg tgttggtagg acctttattt
    13681 ggttcttccg ctccagctcc ggttacggta ggtaacacgg cggcaccagc tgtggctgca
    13741 cccgctccgg caccggtgaa gaaaagcgag ccgatgctca acgacacgga aagttatttt
    13801 aataccgcga tcaaaaacgc tgtcgcgaaa ggtgatgttg ataaggcgtt aaaactgctt
    13861 gatgaagctg aacgcctggg atcgacatct gcccgttcca cctttatcag cagtgtaaaa
    13921 ggcaaggggt aattacgccc cacagtgctg attttgcaac aactggtgcg tctcctggcg
    13981 cacctttttt tatgcttcct tcctgggata tgagcgattt tttatagtaa ctcacttctt
    14041 cttcactaag aatatccatt atctcaatgc cttatcagag attcttttcc tttcgccggt
    14101 agtgtctgga cattcaggct acttttccag gttattttat ttctgttatg cagaggtttt
    14161 atgataagtc atatcctaaa ttctggcggc aataactctt tgatgaaaca tgatgtggtg
    14221 caaggaaata atatagtaga tcttgattta ctacgtaatt tcaatggggt gccaggttta
    14281 aacagagata actttattta tatcagcaat attttttcaa atataaaaca acggaacgaa
    14341 aaaatcatgc aataaatctg tttcgtgaag tctcaatcag taatgatact ataagtgtaa
    14401 aattctacag aaatgaaaaa aattgaatgc gcttgcaatt ttctgatgga taaagatgcg
    14461 caggggtata tcgacctgtc tgatttggat ttaacaagtt gtcattttaa aggtgacgtt
    14521 atatcgaagg tgtctttttt atcatcaaat ctacaacatg taacattcga atgtaaagaa
    14581 attggggatt gcaattttac tactgcaata gttgataatg tcatatttag atgtcgacgt
    14641 ttacacaatg tgatttttat caaagcgagt ggtgaatgtg tcgatttcag caaaaatatt
    14701 cttgatacag ttgacttctc gcagagtcaa cttactcaga gtaattttcg cgaatatcag
    14761 attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac cagagcagag
    14821 tttctgtctg ccaaagaaat atcatttatt aaatcgaata tgacagctgt tatgtttgat
    14881 catgtgcgaa tatcgacagg gaattttaaa gattgcatta cagaacaatt ggaattaact
    14941 attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa taacattata
    15001 aaaatgattg atacattgcc agataatgca atgatattga aatccgttct ggcagtaaaa
    15061 ctggttatgc aattaaaaat tcttaatatt gttaataaaa actttattga gaatatgaag
    15121 aaaacattta gccattgtcc ttatataaaa gatccaatta tacgcagtta tatccattct
    15181 ggtgaagata acaagttcga tgattttatg cgtcaacatc gattcagcaa ggtggatttc
    15241 gatacccaac agatgatcca ttttattaac aggtttaata tgaataaagg gctgattgat
    15301 aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac ggatgatatg
    15361 atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga tgtttcacct
    15421 ctatttatag aaattgaaga taatttaaga acctttaaca cgaatgaatt aacacgaaag
    15481 gataatatct ttatcctgtt ctcctctgcc gatgatgggc cagttatggt ggtaagctcc
    15541 cagcgcttac atgatatgtt gaatcctaca aaagatacca attggaattc cacgtgtatc
    15601 tataaatcca gacataagat gttgcctatt aatcttactc aggaaacact ttttagctcc
    15661 aaatctcatg gtaaatatgc gcttttccca atttttactg cgagttggcg agctactcgt
    15721 ataaagaata taggtattta agtaaaggaa aacatcaccg ttcctggcat catgggcggt
    15781 gatgccccta cggttgccct cgccagcacg ggcatcggca aagcgtaagg ttcaacagcg
    15841 gtttaccact tcatgcgatt gttgcgtttt tgttgcgcat tagatcactt aatttgcttt
    15901 acatctcccg taaacacttt tctgcgatac aatgccttta cgttatgtaa cggagagttc
    15961 ggcatgtcac accccgcgtt aacgcaactg cgtgcgctgc gctattttaa agagatccct
    16021 gccctggatc cgcaactgct cgactggctg ttgctggagg attccatgac aaaacgtttt
    16081 gaacagcagg gaaaaacggt aagcgtgacg atgatccgcg aagggtttgt cgagcagaat
    16141 gaaatccccg aagaactgcc gctgctgccg aaagagtctc gttactggtt acgtgaaatt
    16201 ttgttatgtg ccgatggtga accgtggctt gccggtcgta ccgtcgttcc tgtgtcaacg
    16261 ttaagcgggc cggagctggc gttacaaaaa ttgggtaaaa cgccgttagg acgctatctg
    16321 ttcacatcat cgacattaac ccgggacttt attgagatag gccgtgatgc cggactgtgg
    16381 gggcgacgtt cccgcctgcg attaagcggt aaaccgctgt tgctaacaga actgttttta
    16441 ccggcgtcac cgttgtacta agaggaaaaa aatatggagt ggagtctgac gcagaataag
    16501 ctgctggcgt ttcatcgctt aatgcgtacg gataagccaa ttggcgcgtt actgctgctc
    16561 tggccaacat tatgggcgtt gtgggtggcg acaccgggcg ttcctcagct ctggatcctg
    16621 gcggtatttg tcgcgggtgt ctggctgatg cgcgctgctg gatgtgtggt gaatgattat
    16681 gctgaccgaa agtttgatgg tcatgttaag cgcacggcga accgaccact tcccagcggc
    16741 gcggtaacag agaaagaggc gcgcgcactg tttgtagtgc tggtactgat ttcgttttta
    16801 ctggtactga cgctgaatac gatgaccatt ctgttgtcga ttgccgctct ggcgttggcg
    16861 tgggtgtacc cgtttatgaa gcggtatacc catctcccgc aagtggtgct gggcgcggcg
    16921 tttggctggt cgattccaat ggcttttgcc gctgtgagtg agtcggtgcc attgagttgc
    16981 tggttaatgt tcctcgccaa tattctctgg gcggtggctt acgacacgca gtatgcgatg
    17041 gttgaccgcg atgatgatgt gaagattggc attaaatcca cggcaatcct gttcggccaa
    17101 tacgataaat tgattattgg tattttgcag attggcgtac tggcactgat ggcgatcatc
    17161 ggtgagttaa atggcttagg ctggggatat tactggtcaa ttgtggtggc tggcgcgctg
    17221 tttgtttatc aacaaaaact gattgccaac cgcgagcgtg aagcctgctt taaagcattt
    17281 atgaataata actatgttgg tctggtactg tttttaggac tggcaatgag ttactggcat
    17341 ttctgatgat gtaaaaaagc cggatgatca tccggctttc ttctgggttg ccttatgcgc
    17401 ggcgcttctc aggcctacac aacacatcgc aatttattga atttgcagat tatggaaggc
    17461 cggataaggc gttttcgccg catccggcaa ttctctctga ttacccttcg ccctgcgtcg
    17521 cactctcaat cgtcaaacgc acgtctgatg taatcaactc cgccagcaac tgataaacct
    17581 tcatcgtttc tgccggttcg gcgtcgccgc tatcgctgat atacccttca tcacgcagcg
    17641 tcagcaccag agaactgaac accgccttgt cgaagaactc cggcgcgttg atgccgtgca
    17701 gcacggagag acgttgcgcg acggtgcggc tctctttctc cagcgtaccg cggttgatcg
    17761 acgggttggc actcaacaac cagaaggtga tgg
//
LOCUS       DAEVZA010000102        17641 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEVZA010000102 DAEVZA010000000
VERSION     DAEVZA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.102"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..142
                     /locus_tag="LAF92_003531"
     CDS             <1..142
                     /locus_tag="LAF92_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="damage-inducible protein YebG"
                     /protein_id="HBI9792481.1"
                     /translation="DEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASH
                     AA"
     gene            209..565
                     /locus_tag="LAF92_003532"
     CDS             209..565
                     /locus_tag="LAF92_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBI9792482.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            892..1551
                     /locus_tag="LAF92_003533"
     CDS             892..1551
                     /locus_tag="LAF92_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBI9792483.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            1760..3820
                     /gene="ptrB"
                     /locus_tag="LAF92_003534"
     CDS             1760..3820
                     /gene="ptrB"
                     /locus_tag="LAF92_003534"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBI9792484.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPESETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(3817..4479)
                     /gene="exoX"
                     /locus_tag="LAF92_003535"
     CDS             complement(3817..4479)
                     /gene="exoX"
                     /locus_tag="LAF92_003535"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBI9792485.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4503..5159)
                     /locus_tag="LAF92_003536"
     CDS             complement(4503..5159)
                     /locus_tag="LAF92_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBI9792486.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(5261..5491)
                     /gene="holE"
                     /locus_tag="LAF92_003537"
     CDS             complement(5261..5491)
                     /gene="holE"
                     /locus_tag="LAF92_003537"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBI9792487.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            5630..6004
                     /gene="yobA"
                     /locus_tag="LAF92_003538"
     CDS             5630..6004
                     /gene="yobA"
                     /locus_tag="LAF92_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBI9792488.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            6008..6880
                     /gene="copD"
                     /locus_tag="LAF92_003539"
     CDS             6008..6880
                     /gene="copD"
                     /locus_tag="LAF92_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBI9792489.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGVWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAIMVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            6893..7234
                     /locus_tag="LAF92_003540"
     CDS             6893..7234
                     /locus_tag="LAF92_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBI9792490.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            7630..8286
                     /gene="pphA"
                     /locus_tag="LAF92_003541"
     CDS             7630..8286
                     /gene="pphA"
                     /locus_tag="LAF92_003541"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBI9792491.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(8287..8478)
                     /locus_tag="LAF92_003542"
     CDS             complement(8287..8478)
                     /locus_tag="LAF92_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBI9792492.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(8583..8819)
                     /locus_tag="LAF92_003543"
     CDS             complement(8583..8819)
                     /locus_tag="LAF92_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBI9792493.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(8937..10376)
                     /gene="rsmF"
                     /locus_tag="LAF92_003544"
     CDS             complement(8937..10376)
                     /gene="rsmF"
                     /locus_tag="LAF92_003544"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBI9792494.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(10456..13089)
                     /gene="yebT"
                     /locus_tag="LAF92_003545"
     CDS             complement(10456..13089)
                     /gene="yebT"
                     /locus_tag="LAF92_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBI9792495.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(13058..14341)
                     /gene="yebS"
                     /locus_tag="LAF92_003546"
     CDS             complement(13058..14341)
                     /gene="yebS"
                     /locus_tag="LAF92_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBI9792496.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            14471..14968
                     /gene="msrC"
                     /locus_tag="LAF92_003547"
     CDS             14471..14968
                     /gene="msrC"
                     /locus_tag="LAF92_003547"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBI9792497.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            15065..15763
                     /gene="proQ"
                     /locus_tag="LAF92_003548"
     CDS             15065..15763
                     /gene="proQ"
                     /locus_tag="LAF92_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBI9792498.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRSTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            15783..>17641
                     /gene="prc"
                     /locus_tag="LAF92_003549"
     CDS             15783..>17641
                     /gene="prc"
                     /locus_tag="LAF92_003549"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBI9792499.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNED"
BASE COUNT         4499 a         4394 c         4510 g         4238 t
ORIGIN      
        1 agacgagcaa cgtgaagccc tttcgctatg gctggcagaa caaaaagatg tgctgagcac
       61 cattctgaaa accggcaaat taccgtcccc acaggtcgtc ggtgctgaat ccgaagagga
      121 agatgcctca cacgcggcct gaagcaactt gcgctcctga caatttgtac catgctttct
      181 cctgaatcat tgtgctatgg agaaaaacat gaaaaaaaga ggggcgtttt tagggctgtt
      241 gttggtttct gcctgcgcat cagttttcgc tgccaataat gaaaccagca agtcggtcac
      301 tttcccaaag tgtgaaggtc tggatgctgc cggaattgcc gcgagcgtaa aacgtgatta
      361 tcaacaaaat cgcgtggcgc gctgggcaga tgatcaaaaa attgtcggtc aggccgatcc
      421 cgtggcttgg gtcagtttgc aggacattca gggtaaagat gataaatggt cagtaccgct
      481 aaccgtgcgt ggtaaaagtg ccgatattca ttaccaggtc agcgtggact gcaaagcggg
      541 aatggcggaa tatcagcggc gttaatggct tctgataatt cgattgaccg ttggcggatg
      601 cggcgcgagt gccttgtccg ccccagaacg caccacaatc gtaggcctga gaagacgcaa
      661 cagcgtcgca tcgggcaatc agcatcaaac aatgccactc aaaacgacaa actactttca
      721 cctaaaatac ccttatcacc tgatatacag taaatacctt acctggcgcg aatttctttg
      781 aataacggcc taccgccccg cttatttgcg catcttttgt ctcgatatac attttctgac
      841 atcctcatcc gctatgctac aggcactaaa aatggttatt gaggagcaaa tatggctaac
      901 tggttaaatc aactgcaatc cctgcttggg caaagcagtt cttctacctc ctcgtctgcg
      961 gatcagggat tgggcaaact gttagtgcca ggcgcattag gcggactggc tgggctgctg
     1021 gtcgcaaata aatcagcacg taaacttctt acgaaatatg gcaccaacgc gttactggtt
     1081 ggcggcggag cggtagcggg tacggtgctg tggaataaat acaaagataa aattcgcgcg
     1141 gcgcatcagg acgaaccgca gtttggcgcg caaagtacgc cgctggatga gcgtacagaa
     1201 cgtttgatcc ttgcgctggt ctttgccgct aaaagtgatg gtcatattga tgccaaagaa
     1261 cgtgcggcaa tcgaccagca attgcgtgaa gccggcgtgg aagagcaggg gcgtgtactc
     1321 attgagcagg caatcgaaca accgctggat ccacaacgcc tggctaccgg ggtccgcaat
     1381 gaagaagagg cgctggaaat ctatttcctg agttgcgcgg ctattgatat tgaccatttt
     1441 atggaacgca gttacctgaa tgcactgggt gacgcgctga aaatccccca ggatgttcgt
     1501 gacggcattg aacgcgatct cgaacagcaa aaacgcactt tggcggaata attccgctct
     1561 ctggatagtc tgaagttgaa tatttcgggc tatccagaac cgcacagcca tttgaacaga
     1621 ctgattttct ctgtctgcat aacattcgat atccgcgcca ttacctgtca tcatctaagc
     1681 aatgactccc ctgtttcgct tgcatccccg gtgagttttg ccacccttat aggatgtttc
     1741 aaccagaaag aacaataaca tgctaccaaa agccgcccgc attccccacg ccatgacgct
     1801 tcatggcgat acgcgcatcg ataattacta ctggctgcgg gacgatacgc gttctcagcc
     1861 agaagtcctg gactacctgc aacaagaaaa tagttacggt catcgggtga tggcctcaca
     1921 acaagccttg caggatcgca tcttaaagga aatcatcgac cgcattccgc aacgagaagt
     1981 ttctgcgccc tacatcaaaa atggctaccg ctatcggcat atttatgaac caggctgtga
     2041 atatgctatc taccagcgtc aatcggcgtt cagtgaagag tgggacgagt gggaaatatt
     2101 gctcgatgcc aataagcgcg cagctcatag tgagttttat tcgatgggcg gaatggcgat
     2161 tacgcccgat aacaccatta tggcgctggc agaagatttt ctttcccgac gccagtacgg
     2221 cattcgtttt cgtaatctgg aaactggtaa ctggtacccg gaactgctgg ataacgttga
     2281 acccagcttt gtctgggcaa atgactcctg gactttctac tatgttcgca agcatccggt
     2341 gacgctgctg ccttatcagg tctggcgtca cgccatcggt acgccagcat cgcaagataa
     2401 actgatctac gaagaaaaag acgataccta ttacgtcagc ctgcataaaa cgacctcgaa
     2461 gcactatgta gtcattcatt tggccagcgc caccaccagt gaagttcgcc tgctggacgc
     2521 ggaaatggcc gatgccgagc cgtttgtttt tctgccgcgc cgcaaagatc acgaatacag
     2581 ccttgatcac taccagcatc gtttttatct gcgttccaac cgccacggca aaaactttgg
     2641 cttataccgt acccgtatgc gtgatgagca acagtgggaa gagttaattc cgccacgcga
     2701 aaacatcatg ctggaagggt ttacgctgtt taccgactgg ctggtggttg aagagcgtca
     2761 gcgcgggtta accagtttgc gccaaattaa ccgcaagacc cgggaagtca ttggtattgc
     2821 ctttgatgat ccggcctatg tgacctggat tgcctacaat ccagaatctg aaaccgcgcg
     2881 attgcgttat ggttattctt ccatgaccac accagacact ttgtttgaac tggatatgga
     2941 taccggtgag cgtcgtgtat taaaacaaac ggaagttcct ggttttgatg cggcgaatta
     3001 ccgcagtgaa cacctgtgga tagtcgcccg tgatggcgtc gaagttccgg tttcgttggt
     3061 ctaccatcgc aaacattttc gcaaaggaca caacccgttg ctggtgtatg gctatggttc
     3121 ttacggcgca agtattgatg ccgatttcag ttttagccgc ttgagtttgt tagatcgtgg
     3181 ctttgtctac gccattgtcc atgttcgcgg cggtggtgag ctggggcaac aatggtacga
     3241 agacggaaaa tttctgaaaa agaaaaatac gtttaatgat tatcttgatg cctgcgatgc
     3301 attgttaaaa ctgggctatg gctctccttc gctttgttat gcgatgggcg ggagtgcggg
     3361 gggcatgttg atgggcgttg caattaatca acgcccggaa ttattccacg gcgttatcgc
     3421 ccaggtaccg tttgttgatg ttgtaacaac gatgcttgat gaatcaattc ctcttaccac
     3481 tggtgagttt gaagagtggg ggaatccgca ggatccgcaa tattacgagt acatgaaaag
     3541 ctacagccca tatgacaacg tcaccgcaca ggcttatccg catttactgg taacgaccgg
     3601 tttgcatgat tctcaggtgc aatattggga accggcaaaa tgggtcgcta aattgcgcga
     3661 gctgaaaacc gatgaccatc ttttattgct ctgtaccgac atggactcag gccatggcgg
     3721 taaatctggt cgctttaaat cgtacgaagg cgtagcgatg gaatatgctt ttctggtcgc
     3781 gctggcgcag ggaacattac ccgctacgcc tgcggactaa gtattttcca gataatgttt
     3841 cagtgttaaa cgcagctccg ggctcatgct gtccaggtta ttaaataacc agcgcagata
     3901 gcccggatcg cgttcggcaa cgtcggaaac cgctttgcca cggtatttgc caaaggtgaa
     3961 ggtcgtcatc aacgacggac gtccggtgat atcggccatc tgttctgccg tccagccgga
     4021 ggtgttcata atatcgataa gcaacgcggc ggtgatataa cagtcataca acgcgcggtg
     4081 atgatgcagg cccggcggcg tctgtacatt gagcttgcgt gttttatata acgccatatt
     4141 gctgtacttg atcccaggcc acaaacgacg ggccagtttc atagtgcaaa tccactcacc
     4201 gggcatctca ggcagtacgc ggcggtcaaa gctggcgtta tgcgcgacat accattcact
     4261 accgtaatag tgtgggatca catcttcaat ccacggttta tcggcgacca tggcttcggt
     4321 gatgcgatga atcgccatcg cttgtggact aataggacga tcggggcgca ccaggtggct
     4381 catggggttg acgatttttc cgtcaatgac atcaacagag gcaatctcaa cgatccctcc
     4441 ctgcaaaccg caggtttctg tatcgataat gcgcaacatg gcatgctcca ggccgaaaaa
     4501 gcctagcgta atggaatgat atcgccttgc caaccctgtg atgaacgctg accgactaac
     4561 aataatgaac cacgatcggc gcgaacgatg agacgaccat attcatccca tagtgcactg
     4621 ttaccacggg catttgccat cagcacggca atcgagtact gatgtgaaaa aaattgtaag
     4681 cgacgggccg acgccagcag gtcgggttct cccagacatt gccctgtagt aaacagtgaa
     4741 caggtcgggt ccatatccat accttgcggt tgttcatcga ccaccgtgat ggttctggaa
     4801 cgacggccca gacatgcacc atgactctga tggtagatcc caggcgtttt tcgccacggt
     4861 gcgaacaccg caattccacg aataaagcga tcgttatatt caacgggaag gccggcaatg
     4921 atggtcattc gccaggtcgt tgcggcataa caaagcgggt ctaacagtga gagatcgggt
     4981 ggggcaggaa gggcacgtcg cgaataatca caccccagta aagaaagcga tggaaaaact
     5041 aacagctgac attgttgcct ggcggcggcc ctcacaaact ccagatgatg agctacctgt
     5101 tcggtgagtg aggttttacg aggctcatat tgcgctgctg caactttcca gaacgacata
     5161 atgacttcct tttcaaaatg actgcaaatt atgggtgcgg tttagaaaat actcataatt
     5221 tagcaggcgt tatgtaagaa agtgtaactc tgtataagtt ttatttaagt ttgggctcgt
     5281 aaggtaaacg tgacagattg accgaagcca aacggtgggc aataagccgc tcgcgaaacc
     5341 agctgcgcaa atgttcaggc tgttcacgtt caaccgcttc agcgatcacc ggcatattgt
     5401 agcgttcttt aaatgccacc ccggccgccg ccaaatcgac attcacttta tccatttctg
     5461 tttgatccag tttagccaga ttcttcagca ttcttaatct cctcatcatt cgcgggcaaa
     5521 agttactaag agtgagccag gatggcaagc cggaattgat gatcctcgac tcagacatta
     5581 gacaaggtta tgctctgtaa aaaagttatt acaaaaagga atagataata tgacttcaac
     5641 tgcacgctcc cttcgctacg cgctggcaat cctgaccact tcgttggtga caccttccgt
     5701 ctgggcacat gcgcatttaa cgcatcagta tcctgcggca aacgcgcaag tgacagctgc
     5761 accgcaggca atcactttaa acttctcgga aggtgttgaa acaggattca gtggtgcaaa
     5821 aatcacgggg ccaaaaaacg aaaatattaa aacattaccg gcgaagcgaa atgagcagga
     5881 ccaaaagcaa ctgattgttc cactggccga ttcgctgaaa cccggaacct acaccgttga
     5941 ctggcatgtt gtttcggtgg acgggcataa aacgaaagga cactacacct ttagcgtgaa
     6001 ataaatcatg ctggcgttta cctggatcgc gctgcgattt atccatttca cctcgctgat
     6061 gctggtcttt ggcttcgcaa tgtacggtgt ctggctggcc cccttaacga ttcgtcgctt
     6121 gctggcgaag cgttttctgc gtttacaaca gcacgctgcc gtctggagtt tgatcagtgc
     6181 cactgcaatg ctcgccgttc agggcgggtt gatgggaacg ggatgggcag atgtattttc
     6241 gccaaacatt tggcaggctg ttttgcaaac gcagtttggt ggcgtctggt tatggcaaat
     6301 tgttctcgcc ctcgtaacgt tgatcgtcgc ccttatgcaa ccgcggaata tgccacgttt
     6361 gctgtttatg ctcactaccg cgcaatttat tctgctggcg ggaatcgggc atgcgacact
     6421 gaatgaaggg gtaacggcga aaatccatca gactaatcac gcgattcacc tgatttgtgc
     6481 ggcggcgtgg tttgggggat tactgccggt gctctggtgt atgcaactca tcaaaggccg
     6541 ttggcgacac caggccattc aggcgctgat gcgtttttcc tggtgcgggc attttgcggt
     6601 gatcggcgta ctggcaagcg gcgtgcttaa tgcattgttg attaccggat ttccccccac
     6661 gcttacgact tactggggcc aactcttatt gctcaaagcc atcatggtga tgattatggt
     6721 ggtcattgcg ctggctaatc gttatgttct tgtaccgcgc atgaggcaag atgaagatcg
     6781 tgcggcaccc tggtttgagt ggatgactaa actcgaatgg gcgataggtg cggtagtgct
     6841 ggtgatcatc agcctgcttg cgactctcga acctttttga tggattacga aaatgatgaa
     6901 aaaaagtatt ctgacgtttc tgttactcac cagttctgca gcggcgctgg ctgcaccgca
     6961 ggtgattacc gtcagtcgtt ttgaagtggg taaagacaaa tgggcgttta atcgcgaaga
     7021 ggtgatgctg acttgcagac cgggtaatgc tttgtatgtc attaacccaa gtaccctcgt
     7081 gcagtatcct ttaaacgata tcgcacaaaa ggaagttgcc agtgggaaga ctaaagccca
     7141 acccatttcg gtgattcaga ttgatgatcc taacaatccc ggcgaaaaaa tgagtctggc
     7201 accgtttata gaacgagctg aaaaactctg ttaattacct aaaatagcct tttgatttcc
     7261 aataaaaaaa ccgcctcagt tctttcacca gaacgggcgg tttttaacat ttcagctgat
     7321 gaccaccacg ctttttattg accattttgc acgcaaactg gaaaacctgg cgtcgtcatc
     7381 tattcttaaa gggcaaggca actaagcctg cattaatgcc aacttttagc gcacggctct
     7441 ctcccaagag ccatttccct ggaccgaata caggaatcgt gttcggtctc tttttatctg
     7501 ttaaaagcca gaagcatttc cttcgctgac tttatagtca accataacac acactctact
     7561 gtctgagtcc agcgtttttt aacattcttg ttaagattat gtgatcttta gcgcgggagg
     7621 aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca tcaatggcga
     7681 catatctggc tttctggcga tatacacggt tgtcttgagc agttgcgccg caaattatgg
     7741 cattgtcgtt ttgatccgtg gcgagattta cttatctcag taggagacgt tatcgatcgt
     7801 gggccgcaaa gtttacgttg tctgcagtta ctggaacaac attgggttcg tgcggtaaga
     7861 ggcaatcatg aacagatggc gatggatgcg ctggcatctc agcagatgtc tttgtggttg
     7921 atgaatggcg gcgactggtt tattgcgctg gcagataatc aacagaaaca agcgaaaacg
     7981 gcgctggaaa aatgtcagca tttgcccttt attcttgaag tacacagtcg taccggcaaa
     8041 catgttattg ctcatgccga ttatccagat gatgtttatg aatggcaaaa ggacgttgat
     8101 ttgcatcagg tcttgtggag ccgctcgcga ttaggtgaac gccaaaaagg gcagggaatt
     8161 acaggtgctg atcatttctg gtttggtcat acaccgttgc gacatcgcgt ggatattggc
     8221 aacctgcatt atattgatac cggtgctgtc tttgggggcg aactgactct tgtgcaattg
     8281 caataattaa aaatcaccgt actcctgtgc aggtcgccag aaaccatcta taaaatcctc
     8341 aatcggaaaa caaccgccat ggcggatccg ttgatcgctc atagaataaa gacactgctg
     8401 ttccgtgttg tagacatcca caacaatatc ttcacaaccg ccatccaggt agcaaacaaa
     8461 aagtaccagc gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa gtttaggaga
     8521 gattttacaa cgggggaata accaggacaa ataacccgcc atgccgacgg gttctttttg
     8581 gatcaggcaa gacgcataat ccaggcatca gattgttgat cgtaacgggt acggtaaagg
     8641 acagagtgtt cgccatttag cagggcaggg atactggttt cagcatccca ggcatcgagt
     8701 tgcatacata aatcggggtc agatttacaa ggaatggtta acgttcgatc acccggcgat
     8761 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
     8821 ttcgtgctct ccatttagct gccgattgtg gtacgaccag tccgatcgca ccatttttca
     8881 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
     8941 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
     9001 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caatacatca
     9061 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
     9121 tgcggtgtca gctcgaatgc attcatatta tcgggtgagg caagagcaat aaccgcttca
     9181 tgctgccagc gataaccttt gttgtgcgtc tcggcaagtt taatccccaa ccgagaaaat
     9241 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
     9301 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
     9361 tgtccagctt cgcgatcttt caccgggctg aacgggaaat tgcccacttt gtatttgggg
     9421 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
     9481 tcgtaaattt gtgggaaaac atgcaaaaag ccttcttcgg tcagcgcttt gtttgcacca
     9541 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
     9601 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtacca
     9661 ccaggacgta atgcatgaaa ggcgctgtcg ataagctccc gttgtgtagc tgcgatttcc
     9721 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     9781 ccagagcagg gagcgtccag caaaatggca tcgaacattt ctggcactgc cgcaccaaac
     9841 acgcggccat caaaatgtgt gagcgcaaca ttactgatgc cacagcggct gatattggca
     9901 tgtaacactt ttacccgact ggcggaaaac tcattggcaa ggattgcccc ttcgttattc
     9961 atccgcgcgg caatttgcgt cgttttggag cctggcgcag ctgcgacatc catcacccgc
    10021 tgtggtgcat tatcgtcagc aaacaaggcg gcaacgggca acattgaact ggcttcctga
    10081 atataaaaca ggccgcttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
    10141 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
    10201 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
    10261 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa atgagagcgt cgaaggcatc
    10321 gcttcgcgca tttgtgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
    10381 atgtaccact ggcaaaaaaa caggcgcgca gtttagcata aacgctccgg cgcaggcacg
    10441 ccggagcagt gggcattatt tgggaagcgc agttccccat tcacgccact ctttcggttc
    10501 actttcctgc aacaggaagt gtttgccttc ctgggctttc ggtgccagtg gcgtacccgg
    10561 aggcgtggcg aaggcgatgc cgccacggat aaactggtta aaggtgccgg ttttcactac
    10621 gccgcccgtc agaccaaagt ccagactgta acccgatgcc aaccagaaga cggaattgtt
    10681 acgcaccagg tgttgatagc gtttactgat gcgcatcgca atcatcacgc gatctgacaa
    10741 tgtccccagc gtcattcctg taaccgtacc gacttccaga ccacggaaca gcacaggcgt
    10801 accaatacct aacgaaccgg cttccggcgc ttcaacaata atgcttaagc catccaggta
    10861 acgcgaatca gtaatggtgg cctcttgtaa ttcaaagtcg cggcgaggat tgccccggcc
    10921 tggttcgacg ttgatatacg gctggaggat agtatctaga tgctcaacgc ccgcggctga
    10981 aatttgcggt gtgaccaccg agaagcgcgt accgccgcgg gcgaaggtct ggacatactc
    11041 cggatagagc accgcctttg cctgtacttc attgcgcgcg gtaatcagat ccagcgtctg
    11101 gatttgcccg atatcaatac cgagatagcg aattggcatc ccgaccgcca gttttccggc
    11161 atcgaaagcg tgaagcgtaa tctgcccacc aaccgcacgg gccgctgttt cggaagcata
    11221 cagaattcgt ttgtcgcctt tacgctgact ggcgctggca ccgctgaggt tatcgaagct
    11281 aatggctccc tttaatgctc tggagagcgg ggatgcctgt acggtcaggc cactaccatt
    11341 cagctgaact ttcgccccgc cttctgccca gaacacgcta ttgctggtca gaaggttgcg
    11401 atactccggc ttaatatgca gatcgatatc aaacgcgtta gctcgcggac ggacggtaat
    11461 aacttcacca acttcaaatt tacggtagag cactaccgat cctgcctgca catccggcag
    11521 cgtctctgca ctcaaactca cggttgtggt gggtaaatcg ctaaggctgt tctccagcgc
    11581 tttttccaga ttggcataca gtggatagct ggctttcatc tcgcctttat cgcccggcag
    11641 aatacgtatc ccgccgttaa tccattctga ggcgctggca ccgagaaact caacgccatc
    11701 cagccccacc ttcacgtcga cacggctgtt gacgacaaat ttgctatcgc cttttaccag
    11761 ttctcgatgc tgaggctcga tggcgacggt aaaggtgacg cctttgctgg tgagtttacg
    11821 atcgataacc tggcctacct gcacgccgtg aagaatgagc ggctgaccag catcaatacc
    11881 gtaactttcc ggcgcggtca gggtcagcgt cagaacatca ggttcatgca gcagtgcttt
    11941 ttcgcctggc acaacaacga actctttgcg tggctcgcca tcgccgggta ccaactcgaa
    12001 ggttttgcca gtcagcaggg cgctgagatt ggcatcgcta agggataatt tcggattacg
    12061 taattcgatg cgggtatttt cacgaagcag ggtaacgacg ctgggatcaa ctgtcatttc
    12121 cccagtgact ttaccaccag gatttaaatc cagtttagtt agctgtccga cttccagccc
    12181 ctgatacatt aacggcgtcg agtcggcggt caatccggcc ccactcggca gttccagttt
    12241 tattattacg ccacgctggc tgtgggccag atcttcatac agaccaaagg tatcttccgc
    12301 ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa ccagtgccgc
    12361 cagactttcc agtttcacct tcgcgccact gacactgacg ttggcatcaa cgccggaaac
    12421 gttccagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga tcaggacatc
    12481 aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc ccaccgggat
    12541 cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agatcgggag cttgcaggtg
    12601 gatcatcaga tcgccgttat ccagccgata cttcggttgg gtatcgagtg cgacaaagtg
    12661 atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga cgagggcgtc
    12721 cagcccggag acacctgcca acgatgcttt tggcgtcacc agccagaatt gagtctcttc
    12781 gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct tacgaagatc
    12841 gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac gaacaggcgt
    12901 acggcccggg acaataccat ccgccgacat aaagtcgatg gtgacggtat taccccggtc
    12961 ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaacg gcagcagcca
    13021 gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag cgggcgtttc
    13081 ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca agaatagtca
    13141 aaattaccgc tgcgccgaaa taaaacgcag ccggtcccat agtaaaagcg aggatctgat
    13201 cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc gaccagcgac
    13261 caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc tggcatttaa
    13321 aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt accagaatac
    13381 tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac ataattccgg
    13441 aaagaattgt atcttcctgc cgtcctccgt tcagataaat gatagaaata ggcaacaggt
    13501 tggcaggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt tgcaaactat
    13561 gacggcgacg caggcgtagc gggatatggc aacgcgggca gcgaccacgc tgatcaggat
    13621 agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc gtagcggggc
    13681 gctgcggata aaatcgctcc cacagctcct cgacattaag atgtgacaac gtcaccgtcg
    13741 ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata tgggcataat
    13801 cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc attacccact
    13861 ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt cggttaccaa
    13921 accataaata tgctatggag gacaccagaa tgaggggggc accaataacg cagaaaaaga
    13981 ccatcgcccc cgttatcgta tcgccctgtt tggtcatttg ccagatgcct tgcatgacgt
    14041 tggcgtcgat acgaatacct aacagccaga tatgcaacag cggttcgccc caggcaaacg
    14101 gcatcagcaa cagcatggtg aaagccattg ccgccaggcg cgttagcgac cagtcgcgcc
    14161 cgtcgcgaat ttttgcctga cagcgcggac aataggcact ctgatgagaa tttatctcgg
    14221 gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc ggcagttcct
    14281 cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg ttaagagcca
    14341 tgacgtgcca aatgtaataa gaattaacat taatattaac ccatgattgg aatgatcttg
    14401 agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa gacaaccaag
    14461 tgattatata atgaacaaaa cagaatttta cgcggattta aatcgcgact ttaacgcgct
    14521 gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt tgttatacga
    14581 gcgtctcact gacgtaaact gggcaggttt ttatttgctt gaggacgata cactggtact
    14641 aggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg gggtgtgcgg
    14701 cactgcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt ttgacgggca
    14761 tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg tgaaaaatca
    14821 gattattggt gttcttgaca tcgatagtac cgtcttcggt cgctttacag acgaggacga
    14881 gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga cggattacaa
    14941 aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta ctggtagcgt
    15001 cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt aatcaggaaa
    15061 tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt ttctggccga
    15121 acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa tcggtatttt
    15181 tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc aattacgatc
    15241 cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac ccggcgcaac
    15301 gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg tagagcatgc
    15361 tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg aacagcaagc
    15421 gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc gccgcgaacg
    15481 caagccacgt tcgaccacgc cacgccgcaa agaaggcgct gaacgtaaac ctcgtgcgca
    15541 aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac agcacacccc
    15601 ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag cgggtcaaaa
    15661 cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc agctgaattc
    15721 gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag gccgggccag
    15781 gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata gcaggccaga
    15841 ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag gaagagacgc
    15901 agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat tatcgccagt
    15961 tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat ctgctcgatt
    16021 acagccacaa cgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag aaaaccgagt
    16081 taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac aatctggcgc
    16141 aaaagcgccg ttttgagcgt taccagtacg ctttgtcggt actggaaaag ccgatggatt
    16201 tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg aaaaacgagg
    16261 ctgagttgaa cgcgctgtgg gacagtaaag tcaaattcga cgagttaagc ctgaagctgg
    16321 caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa tttgccattc
    16381 gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg gcgtttgcgc
    16441 gtgaaatcga cccgcatacc aactatcttt ccccgcgtaa taccgaacag ttcaacactg
    16501 aaatgagttt gtcgctggaa ggtattggcg cagtgctgca aatggatgat gactacaccg
    16561 ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc agcgttggtg
    16621 acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt ggctggcgtc
    16681 ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt ctggaaattt
    16741 tacctgctgg taaagggacc aagacccgca ctgtaacatt gacccgtgaa cgtattcgtc
    16801 tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa gtcggcgtgc
    16861 tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa ctgcagaaac
    16921 tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc ggtggggcgt
    16981 tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt gttcaggtcc
    17041 gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg ttctataaag
    17101 gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc tttgccgcgg
    17161 caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt aaaggcaccg
    17221 ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct gaatggccag
    17281 cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc ggcagtacgc
    17341 aacgtaaagg cgtaacgcca gacatcatca tgccgacggg taatgaagaa acggaaacgg
    17401 gtgagaaatt cgaagataac gcgctgccgt gggatagcat tgatgccgcg acttatgtga
    17461 aatcaggcga tttaacggcc tttgggccgg agctgctgaa ggaacataat gcgcgtatcg
    17521 cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac gctatgaagg
    17581 acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat aatgaagatg
    17641 a
//
LOCUS       DAEVZA010000103        17587 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEVZA010000103 DAEVZA010000000
VERSION     DAEVZA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.103"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..361
                     /gene="yghA"
                     /locus_tag="LAF92_003550"
     CDS             <1..361
                     /gene="yghA"
                     /locus_tag="LAF92_003550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBI9792500.1"
                     /translation="PKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVAE
                     KGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQES
                     SYVTAEVHGVCGGEHLG"
     gene            complement(633..1058)
                     /gene="exbD"
                     /locus_tag="LAF92_003551"
     CDS             complement(633..1058)
                     /gene="exbD"
                     /locus_tag="LAF92_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBI9792501.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETTKAK"
     gene            complement(1065..1799)
                     /gene="exbB"
                     /locus_tag="LAF92_003552"
     CDS             complement(1065..1799)
                     /gene="exbB"
                     /locus_tag="LAF92_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBI9792502.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            2051..3238
                     /gene="metC"
                     /locus_tag="LAF92_003553"
     CDS             2051..3238
                     /gene="metC"
                     /locus_tag="LAF92_003553"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBI9792503.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            3378..4037
                     /locus_tag="LAF92_003554"
     CDS             3378..4037
                     /locus_tag="LAF92_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI9792504.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(4077..4976)
                     /gene="yqhC"
                     /locus_tag="LAF92_003555"
     CDS             complement(4077..4976)
                     /gene="yqhC"
                     /locus_tag="LAF92_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBI9792505.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            5170..6333
                     /gene="yqhD"
                     /locus_tag="LAF92_003556"
     CDS             5170..6333
                     /gene="yqhD"
                     /locus_tag="LAF92_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBI9792506.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            6438..7265
                     /gene="dkgA"
                     /locus_tag="LAF92_003557"
     CDS             6438..7265
                     /gene="dkgA"
                     /locus_tag="LAF92_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBI9792507.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            7465..8391
                     /locus_tag="LAF92_003558"
     CDS             7465..8391
                     /locus_tag="LAF92_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBI9792508.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            8442..8699
                     /gene="yqhH"
                     /locus_tag="LAF92_003559"
     CDS             8442..8699
                     /gene="yqhH"
                     /locus_tag="LAF92_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBI9792509.1"
                     /translation="MKTIFTVGVVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(8742..10961)
                     /locus_tag="LAF92_003560"
     CDS             complement(8742..10961)
                     /locus_tag="LAF92_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBI9792510.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(11072..12484)
                     /gene="ftsP"
                     /locus_tag="LAF92_003561"
     CDS             complement(11072..12484)
                     /gene="ftsP"
                     /locus_tag="LAF92_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBI9792511.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(12559..13296)
                     /gene="plsC"
                     /locus_tag="LAF92_003562"
                     /gene_synonym="parF"
     CDS             complement(12559..13296)
                     /gene="plsC"
                     /locus_tag="LAF92_003562"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBI9792512.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(13530..15788)
                     /gene="parC"
                     /locus_tag="LAF92_003563"
     CDS             complement(13530..15788)
                     /gene="parC"
                     /locus_tag="LAF92_003563"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBI9792513.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYGAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYADDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(16334..16816)
                     /locus_tag="LAF92_003564"
     CDS             complement(16334..16816)
                     /locus_tag="LAF92_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI9792514.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(16869..17261)
                     /gene="ygiW"
                     /locus_tag="LAF92_003565"
     CDS             complement(16869..17261)
                     /gene="ygiW"
                     /locus_tag="LAF92_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBI9792515.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            17413..>17587
                     /locus_tag="LAF92_003566"
     CDS             17413..>17587
                     /locus_tag="LAF92_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBI9792516.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMD"
BASE COUNT         4277 a         4683 c         4464 g         4163 t
ORIGIN      
        1 accgaaaggt gcaagtatca tcaccacttc gtcaatccag gcataccagc caagtccgca
       61 tttactggac tatgcggcta cgaaggcggc gattctgaac tacagccgtg gcctggcaaa
      121 acaggtcgcg gagaaaggta ttcgggtgaa tattgtcgcg ccaggcccga tctggacagc
      181 actgcaaatt tccggcggac aaacgcagga taagatcccg cagtttggtc agcaaacgcc
      241 gatgaagcgt gcggggcaac cggcggaact ggcccctgta tatgtttatc tggcaagtca
      301 ggagtcgagc tacgtcaccg cagaagtgca cggtgtgtgc ggcggcgagc atttaggtta
      361 aaaaaatgcc cggttgtgaa aagcaaccgg gcatcattgt gaattcactc ttacctgatg
      421 tggcgaaaac gccttatccg cctacaaacg catgaagatt caacatgttg caatagattt
      481 tgtaggcctg ataagcgtag cgcatcaggc attgagcacc taatgccgga tgcgtcgtga
      541 acgccttatc cggcctacaa acgcacgaag attcaacctg ttgcaataga ttttgtaggc
      601 ctgatgagcg tagcgcatca ggcaattcta ctttacttcg ctttggtggt ttcttcgccg
      661 accagaccta tcttcaggta acccgcctga tgcagcgtat ccattacctt catcaacgtc
      721 tcgtaatcga cggttttgtc cgctcggaag aagatggtgg tgtctttctt gccttcggtt
      781 aacgcattca acgccgtaat cattgtttca tcggtgaccg gatcgttacc gataaacatc
      841 gagttgtctg ccttcaccga cagataaacc ggtttttccg ggcgcggctg cggcgtgctg
      901 gtagaagcag gcaagttcac cttcacatct accgtcgcta acggtgccgc caccataaag
      961 ataatcagca gaaccaacat cacgtcgata aacggcgtca cgttgatatc atgcatttcg
     1021 ccgttatcgt cgaggttttc gttaagatgc attgccatcg gatattatcc tgcgcgtaat
     1081 ttttgtgcga cacgaaccgg atgcgcagcg gcgctggctt ccagatccag gtcacggctt
     1141 tgcagcaaca atacctgcgc tgcaacatca cccagcatcg ctttaaagcc gccaatctgg
     1201 cgtgcaaata cgttatagat aacgaccgca ggaatcgctg ccacgaggcc gattgccgtt
     1261 gctaacagag cttctgcgat acccggcgca acgactgcca ggttagtggt ctgcgtttgc
     1321 gcgataccaa taaagctgtt catgatgccc cagaccgtac caaacagacc aacgaacgga
     1381 gaaatcgcgc cgatggttgc cagatagccg ttaccgcggc ccatttgacg acccactgcg
     1441 gcgacccgac gctccagacg gaagctggta cgttctttaa taccttcgtt atcgtcgctg
     1501 ccttctgaca gttccagctc attctgcgct tcattgagca aatgcaggct taagcttttg
     1561 ctaccaaaat cagcggcgat atcgttggcc tggtttaagg aacgcgcttc agccagcagt
     1621 tgctgctcgc gcttaaggcg acgcttctga ttgaagaact ctacgctctt actgaagaag
     1681 attgcccagg tgactacgga ggccaaaata agcccaatca tcacgcactt aacgacaata
     1741 tcggcgtgct gatacatacc ccagacggaa aggtccgtct gcattaaatt attacccact
     1801 gtgtatctcc aggacgcaag tcacaaaatc tgcgcataat aatatcaaaa tgacgtcgaa
     1861 ttgatagtcg ttctcattac tatttgcata ctgccgtacc tttgctttct tttccttgcg
     1921 tttacgcagt aaaaaagtca ccagcacgcc atttgcgaaa attttctgct ttatgccaat
     1981 tcttcaggat gcgcccgcga atattcatgc tagtttagac atccagacgt ataaaaacag
     2041 gaatcccgac atggcggaca aaaagcttga tactcaactg gtgaatgcag gacgcagcaa
     2101 aaaatacact ctcggcgcgg taaatagcgt gattcagcgc gcttcttcgc tggtatttga
     2161 gagtatggaa gccaaaaagc acgcgacgcg caatcgcgcc aatggcgagt tgttctatgg
     2221 acggcgcgga acgttaaccc atttttcctt acaacaagcg atgtgtgaac tggaaggtgg
     2281 cgcaggctgc gcgctatttc cctgcggggc ggcagcggtt gctaattcga ttcttgcttt
     2341 tgtcgaacag ggcgatcatg tgttgatgac caacaccgcc tatgaaccga gtcaggattt
     2401 ctgtagcaaa atcctcagca aactgggcgt aacgacatcg tggtttgatc cgctgattgg
     2461 tgccgatatc gttaagcatc tgcagccaaa cactaaaatc gtgtttctgg aatcgccagg
     2521 ctccatcacc atggaagtcc acgacgttcc ggcgattgtt gccgccgtac gcagtgtggt
     2581 cccggatgcc atcattatga tcgacaacac ctgggcggcc ggagtgctgt ttaaggcgct
     2641 ggattttggc atagatgttt ccattcaggc cgccaccaaa tatctggttg ggcattcaga
     2701 tgcgatgatt ggcactgccg tgtgcaatgc ccgttgctgg gagcagctac gggaaaacgc
     2761 ctatctgatg ggccagatgg tcgatgccga taccgcctat ataaccagcc gtggcctgcg
     2821 cacattaggt gtgcgtttgc gtcaacatca tgaaagcagt ctgaaagtgg ctgaatggct
     2881 ggcagaacat ccgcaagtag cgcgagttaa ccaccctgct ctgcctggca gtaaaggcca
     2941 cgaattctgg aaacgagact ttacaggcag cagcgggcta ttttcctttg tgcttaagaa
     3001 aaaactcaat gatgaagagc tggcgaacta tctggataac ttcagtttat tcagcatggc
     3061 ctactcgtgg ggcgggtatg aatcgttgat cctggcaaat caaccagaac atatcgccgc
     3121 cattcgccca caaggcgaga tcgattttag cgggaccttg attcgcctgc atattggtct
     3181 ggaagatgtc gacgatctga ttgccgatct ggacgccggt tttgcgcgaa ttgtataaca
     3241 ttgccacttt tggacaattt tgcagacatt ttattgtgaa aagtcttaaa ttgttgcgtc
     3301 cgggatcaag gcgtcccgga cgattcagga gtacaatagg cagataaagg cttaaacgct
     3361 gttccacagg aaagtccatg gctgttattc aagatatcat cgctgcgctc tggcaacacg
     3421 actttgccgc gctggcggat cctcatattg ttagcgttgt ttactttgtc atgtttgcca
     3481 cgctgttttt agaaaacggc ctgctgcccg cctcattttt gccaggcgac agcttgttga
     3541 tactggcagg cgcattgatt gcccaggggg ttatggattt tctgcctacg attgcgattc
     3601 tgaccgccgc agcaagtctg ggctgctggc taagttatat tcaggggcgc tggttaggga
     3661 ataccaaaac ggtgaaaggc tggctggcac agcttcctgc taaatatcac cagcgcgcca
     3721 cctgcatgtt tgaccgccac ggtctgctgg cgctgctggc tggacgtttt cttgcatttg
     3781 tccgtacgct gctgccaacc atggcgggaa tttccggtct gccaaaccgc cgcttccagt
     3841 ttttcaactg gttaagtgga ttgctgtggg tcagcgtggt aaccagtttt ggctatgcct
     3901 taagtatgat tccgttcgtt aaacgccatg aagatcaggt aatgacgttc ctgatgatcc
     3961 tgccaattgc cttgttaacc gctggcttgt taggcacgct gtttgtggtg attaaaaaaa
     4021 aatactgtaa cgcctgacga ttttccccgt tcccggttgc tgtaccggga acgtatttaa
     4081 ttcccctgca tcgcccgcat tcttgccgca tcttcccccg gcgtcacacc gaagtaacgt
     4141 ttaaactcac ggctaaattg cgatgcgctt tcatagccga cgcgcatcgc tgctgcgctg
     4201 gccttcatgc cgtcatggat gatcatcatc cgcgccttat gcagacggta attcttcaaa
     4261 tactgcaacg gcgaggtgct ggtgacagac ttaaaattat ggtggaacgc cgatacgctc
     4321 atgttggctt ctgccgccag ttgctcgacg ctcaggtttt cggtgtattt attctcaatc
     4381 cgtttcagca cgcggctaat cagactgaag tgagtctggc gactgaccag cgccagtaac
     4441 gcgccgccgc aaggtccggt cagcacgtag tacagaattt cgcggatgat ctgtttgccg
     4501 agaatacgcg catccagtgg tcgctccatc acgtcgagta accgctccgc cgcgcataaa
     4561 atctcttctg ataacgtggc ggagttaatc ccgctggctg ccatcgacgg ctggaaatgc
     4621 tcatcttcgc caatgtccat caacagttcc tgtaactgca aaatatcgac attgagacgc
     4681 aaccctgcca gcggcacctc tgacgtggca taggtttcgc actcaaacgg caacggcacc
     4741 gtcagcagca ggtattcatt ggcatcataa cgaaacacgc gttcattgat ataaccgatt
     4801 ttatgtccgg aaaagagaat tatgatgcca ggctcgtaca tcaccggtgt acgtgcgaaa
     4861 ggcgtctcgc catacaacaa acgcacatcg ggcaacagtt ctgacaaact attttcttta
     4921 tttttcagtt tattaacttt atccgccagc aagcggcaaa tctcttcacg tttcatatcg
     4981 cgtaatttct taggaataat gcggcaattt gattgtgcgc aattttgtag catttctcca
     5041 gcactctgga gaaataggca agacattggc agaaatgagc attgagagcc agggcgctgg
     5101 cgatcacaat gaaaaacatc aggcagatcg ttctctgccc tcatattggc ccagcaaagg
     5161 gagcaagtaa tgaacaactt taatctgcac accccaaccc gcattctgtt tggtaaaggc
     5221 gcaatcgctg gtttacgcga acaaattcct cacgatgctc gcgtattgat tacctacggt
     5281 ggcggcagcg tgaaaaaaac cggcgttctc gatcaagttc tggatgccct gaaaggcatg
     5341 gacgtgctgg aatttggcgg tattgagcca aacccggctt atgaaacgct gatgaacgcc
     5401 gtgaaactgg ttcgcgaaca gaaagtgact ttcctgctgg cggttggcgg cggttctgta
     5461 ctggacggca ccaaatttat cgccgcagcg gctaactatc cggaaaatat cgatccgtgg
     5521 cacattctgc aaacgggcgg taaagagatt aaaagcgcca tcccgatggg ctgtgtgctg
     5581 acgctgccag caaccggttc agaatccaac gcaggcgcgg tgatctcccg taaaaccaca
     5641 ggcgacaagc aggcgttcca ttctgcccat gttcagccgg tatttgccgt gctcgatccg
     5701 gtttatacct acaccctgcc gccgcgtcag gtggctaacg gcgtagtgga cgcctttgta
     5761 cacaccgtgg aacagtatgt taccaaaccg gttgatgcca aaattcagga ccgtttcgca
     5821 gaaggcattt tgctgacgct aatcgaagat ggtccgaaag ccctgaaaga gccagaaaac
     5881 tacgatgtgc gcgccaacgt catgtgggcg gcgactcagg cgctgaacgg tttgattggc
     5941 gctggcgtac cgcaggactg ggcaacgcat atgctgggcc acgaactgac tgcgatgcac
     6001 ggtctggatc acgcgcaaac actggctatc gtcctgcctg cactgtggaa tgaaaaacgc
     6061 gagaccaagc gcgctaagct gctgcaatat gctgaacgcg tctggaacat cactgaaggt
     6121 tccgacgatg agcgtattga cgccgcgatt gccgcaaccc gcaatttctt tgagcaatta
     6181 ggcgtgccga cccacctctc cgactacggt ctggacggca gctccatccc ggctttgctg
     6241 aaaaaactgg aagagcacgg catgacccaa ctgggcgaaa atcatgacat tacgctggat
     6301 gtcagccgcc gtatatacga agccgcccgc taagcttttt acgcctcaaa ctttcgtttt
     6361 cgggcatttc gtccagactt aagttcacaa cacctcaccg gagcctgctc cggtgagttc
     6421 atataaagga ggaacgtatg gctaatccaa ccgttattaa gctacaggat ggcaatgtca
     6481 tgccccagtt gggactgggc gtctggcaag caagtaatga ggaagtaatc accgccattc
     6541 aaaaagcgtt agaagtgggt tatcgctcga ttgataccgc cgcggcctac aagaacgaag
     6601 aaggtgtcgg caaagccctg aaaaatgcct cagtcaacag agaagaactg ttcatcacca
     6661 ctaagctgtg gaacgacgac cacaagcgcc cccgcgaagc cctgctcgac agcctgaaaa
     6721 aactccagct tgattatatc gacctctact taatgcactg gcccgttccc gctatcgacc
     6781 attatgtcga agcatggaaa ggcatgatcg aattgcaaaa agagggatta atcaaaagca
     6841 tcggcgtgtg caacttccag atccatcacc tgcaacgcct gattgatgaa actggcgtga
     6901 cgcctgtgat aaaccagatc gaacttcatc cgctgatgca acaacgccag ctacacgcct
     6961 ggaacgcgac acacaaaatc cagaccgaat cctggagccc attagcgcaa ggagggaaag
     7021 gcgttttcga tcagaaagtc attcgcgatc tggcagataa atacggcaaa accccggcgc
     7081 agattgttat ccgctggcat ctggatagcg gcctggtggt gatcccgaaa tcggtcacac
     7141 cttcacgtat tgccgaaaac tttgatgtct gggatttccg tctcgacaaa gacgaactcg
     7201 gcgaaattgc aaaactcgat cagggcaagc gtctcggtcc cgatcctgac cagttcggcg
     7261 gctaacatgc aaattctccc ggtggcggta atgttccgct accggacttt tcagaaatca
     7321 tttattcccc tcgcgtcccg cccgttgtta ctcttccttg ttcaggaatg ccaaatataa
     7381 ggacatcatc atgcagagcc ggaagctctt aaaagaacaa ctcatctata tccgggataa
     7441 acgcaacgga gaggtgaaaa acagatgaaa ataatacttc tgtttttagc agccctggca
     7501 agttttaccg tacacgcaca gcccccctca ctgaccgtag aacaaacagt ccggcatatt
     7561 tatcagaact ataaatcaga tgccactgcc ccttattttg gtgaaaccgg agagcgggcg
     7621 ataacttctg cgcgtattca acaggcgctt accctgaacg acaatcttac gctgccgggc
     7681 aatattggct ggctggatta tgatccggtt tgtgattgtc aggattttgg cgatctggtg
     7741 ctagaaagcg tagcgataac tcaaactgac gccgatcatg ccgatgccgt tgtgcgcttt
     7801 cgtatcttta aagatgataa agaaaagacc acgcagacac tgaaaatggt ggcggaaaat
     7861 ggtcgttggg tcattgacga tattgtcagc aatcatggca gcgtcttaca agcagttaat
     7921 agcgagaatg aaaaaacgct ggccgcttta gcttcgttgc aaaaagaaca gccggaagcc
     7981 tttgttgccg aactctttga acatattgct gattatagct ggccgtggac gtgggtggtt
     8041 tccgactctt accgccaggc ggttaatgcc ttctataaaa ccaccttcaa gacggccaat
     8101 aatcccgatg aagatatgca aatagaacgg caatttattt acgacaatcc gatctgtttt
     8161 ggcgaagagt cgctattttc acgcgttgat gaaattcgag tcctggagaa aaccgccgat
     8221 tccgcccgca ttcatgttcg ttttacgctg accaatggca acaacgaaga gcaagaactg
     8281 gttttacagc ggcgcgaagg caagtgggaa atcgctgatt ttatccgccc gaacagcggc
     8341 agcctactta agcagattga ggcaaaaacc gccgccagat taaagcaatg agctgaatta
     8401 aataacaatt agccggaaca ataaataaaa gggaacacta tatgaaaacg attttcaccg
     8461 tgggagttgt tgttctggca acctgcttgc tcagtggctg cgtcaatgag caaaaggtca
     8521 atcagctggc gagcaatgtg caaacattaa atgccaaaat cgcccggctt gagcaggata
     8581 tgaaagcact acgcccacaa atctatgctg ccaaatccga agctaacaga gccaatacgc
     8641 gtcttgatgc tcaggactat tttgattgcc tgcgctgctt gcgtatgtac gcagaatgat
     8701 aaaaaaatcc ccggcagcat gtcagttgcc ggggattttt tttaacgtcc aaccgccgct
     8761 ttagggcgtt tcttcgcacc agcattcacc ggacgagatt gcgtagacga cgcttttttt
     8821 gccgtagcag gcgtctgacg ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg
     8881 gtattgcggt tctggcgacg agcttcacgc atctcttcaa tggttggcgc aggcactaag
     8941 caatcgcgac ggctgccaat cagatgcttt ttgcccatcg cttccagcgc ctggcggatt
     9001 aacggccagt ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg acgctgcttg
     9061 tcgcccttcg gtacgaagac gtcttcactc ttataaccaa tcttcgccag cgggtttttc
     9121 ccggtgtaat acatggtggt tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg
     9181 tcgagacgga aacgatgctt tttcagccac agcgccagat tcaccatatc ttcatcacgc
     9241 gtaccggggt gcgcggagat gaaatagggg atcagatact gctctttacc tgcctgtttt
     9301 gagtaagtat cgaacagctc tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc
     9361 ttcgataacg gcccttcttc ggtatgttcc ggggcaatct tcagataacc gccgacgtga
     9421 tgggtcgcca actctttgat atagcgcgga tcttccacgg cgatgtcata acgtacgcct
     9481 gaggcgatga ggattttttt aatgcctttc agatcacgcg cacggcgata gaggttgatc
     9541 gtcggttcgt ggttagtgtc catgtgcgga caaatatccg gataaacgca cgacaaacgg
     9601 cgacaagttt gttcagcgcg tggcgatttg cagcgcaaca tatacatgtt ggcagttggc
     9661 ccaccgagat cggaaatcac gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc
     9721 tcattaatga tcgaatcttc cgaacggctc tgaataatgc gcccttcgtg ctcggtaata
     9781 gaacagaaag aacagccgcc aaagcagccg cgcataatgt tgaccgaaaa acggatcatt
     9841 tcgtaagccg gaatacgggc attgccatag gccggatgtg gcacgcgctt gtacggcagc
     9901 gcaaaaacgc tgtccatctc ttcggtagaa agcgggatag caggcgggtt gatccacaca
     9961 tagcggtcac cgtgtttttg catcaatgcg cgggcgcagc ctgggttggt ttcgtggtgc
    10021 agaatgcgcg aagcatgggc gtacagcact ttatcgccct tcactttctc gaaagaaggc
    10081 agcaacacgt aggttttttc ccacggtttc gggcgcggtg gctgcacggt tacggctttg
    10141 gcttcctgct ttttcggtgc caccggtttg ttatccgcgc acggcaaatc ttcaccatac
    10201 ggatgcggga ttgggtcgat ttttccaggg gtatcaagac gggtggaatc cacgccgctc
    10261 cagccaggca gcgcctcttt cacgataatc gcggtattac gcacatcgcg gatttcacta
    10321 atcggctcgc ccatcgccag acggtgcgcc acctccacca gcggacgctc accgttacca
    10381 aacatcagca tgtcggcttt cgaatccacc agcacggaac ggcgcacggt atcggaccag
    10441 taatcataat gtgcggtacg gcgcagacta gcctcaatac cgccgaggat caccggtaca
    10501 tctttccacg cctctttaca acgctgggta taaaccagtg tggcgcgatc cgggcgctta
    10561 cccgcgacgt tatccggcgt gtaggcatcg tcatgacgta aacggcgatc ggcggtataa
    10621 cggttgatca tcgaatccat gttgccagca gtaacaccga aaaacagatt cggtttaccc
    10681 agacgcatga aatcgtcttt gctgctccag tctggctggg cgatgatccc gacgcgaaag
    10741 ccctgtgctt ccagcatacg accgcaaatc gccatcccga agcttgggtg atcgacatac
    10801 gcgtcgccag taaccaaaat gatgtcgcag ctatcccagc caagttgatc catctcttca
    10861 cgagacatcg gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg cggccaggag
    10921 aacaggtcgc gatccggttg gatcagggag atagagctca ttttgcttcc agaaatgata
    10981 aaaaaataat caaaggccgg ggattataag ccggaacgaa agagaaatcg aaaggtattc
    11041 catactcgcc ctcctcgggc gagtatgaag attacggtac cggattgacc aaaagttgcc
    11101 ctatcgagcc acggtccgcc atttccagcg tctgactgtt gaagtagaac gggaagtgcg
    11161 cccaggaagg ctgaccgaaa taaacaagca gctccacttg tccgtcaacc caaacggtat
    11221 ctttccagcc tctgtcttcc gggaacggca tcgcgccatt cacgttacgg atctggaaca
    11281 ttacgccttc aatatggaac gcttgcggct cgtccgcgcg taccgtccag cgttcccacg
    11341 ttccttgctg cgcggtgaca tcaatacggt tgacgtccca cagctgtcca ttaatacccg
    11401 ggtcatcacc cagactgata tcgcgactgc gaattggcga accagccatg atttcagttg
    11461 gcagcaagcg catcggaaga ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg
    11521 ttagcaccag ggtagaaacc agaatactgg atggctcaaa gaagccgcga atacgatcaa
    11581 caatgctcgc cgcttcgcca caggtgatcg acacttcatc gccgttgctc atatccacca
    11641 gaatctcgcg gcgctcgccc ggtgccagcg aaagttgctt caccgataca ggagcaggca
    11701 ggaatccctg atcgccagaa atcacatgta acgggcgacc atcgctcatc tgtagttgat
    11761 agcgacgaga gttcgacgcg ttcagcaggc gcaagcgcac ccagccacgc gagacttcaa
    11821 cgtacgggct ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg
    11881 gttcgttgta ttctggcgta ccaaagttat ccagccgttt atcctggata atgaccggaa
    11941 aatcatccac accataatgg ttggggatag gcagcgactt gctgacttca tcttccacca
    12001 gccacattcc ggcaaggccg ttatagacct gctgagccgt gcggttggga gtattggcgt
    12061 gataccacag agtagctgcg ttctggcgaa tgggcagtac gggtgcccag tcagcgtttg
    12121 gcgacatcat ccgtgccgga ccgcccatca gcgggcctgg tacctgtagc ccggccaccg
    12181 tcattgagac attttctgtc aggcggttgc tgtaaataag cttaacatcg tcgcccttcc
    12241 agacgcggat agtcggcccc aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc
    12301 ctggcgtaaa tgaccagtgc gcacgttgta cagtcataaa cagcggttgc ccacggcgag
    12361 attcaagcag cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg
    12421 gaacagcgcc tgcacaaagt gcaatccccg atgcctgaat gaactgacgc cgactgagtg
    12481 acatatttgc tccatgtaaa actggctaaa gtaataccgt gcaggaaagt gttccccgca
    12541 aaaatacaga ttgccgactt aaacttttcc ggcggcttcg cgttctgcga cttctttatc
    12601 gagctcggcg attttttgtt ccattatcga acgacaatgg gcagccagct cacgaacctg
    12661 atctttgcca tactgactga cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt
    12721 gtgcagtcga ttaagattaa tcttattcga agttgtagag acgcacacgg gaataatcgg
    12781 gacgcccgcc gcaattgccg cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc
    12841 acggctgcgg gttccttccg ggaacatcca gatggaaata cggcgttttt tgaaatgatt
    12901 cacgacttcc gcaatggtgc cgtgagcttt agtgcgattg tttctgtcga tcaataagtt
    12961 gccggttaac cagtacaact gcccgaagaa ggggatccac agcaagctct ttttacctac
    13021 cgtcaccgtc ggcggttgca caatgttcga tgctgtcacc atgtcatagt tgttctggtg
    13081 gttagcgata tagatagcat tgccgtagct ttcagcatct gcaggtttgc ggcactcaac
    13141 tttcaggccg aacagcggcg caagacggcc aaacatatgc ccaaaggtgg ccacatgttt
    13201 cgggttacgc gggctgaaaa ggcagtaaat ggagccgaat acacagacta agatgctgta
    13261 aatcacggta ataataagac gaaagatata tagcataaca ccctctgaag gtctgagaga
    13321 ctggcattgt acgtcacctg aaatcaggtt agggcattgt taaaagccgc tctctggaaa
    13381 agcgtattgt taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt
    13441 ccgggggttg aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgcaag
    13501 cgggaggaaa cagcgccctc cccggcatat tactcttcgc tatcaccgct gctggcacgg
    13561 cgaggagagt cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg
    13621 cgtccacgtt cgccagtgac tttctgtagc tcttccggac gcagtttaat tttgcgtttc
    13681 ccaacatgaa tggtcagcgt gctttgcggc ggcagaacgt acaattgcgc cagaccatct
    13741 tctccacgcg cggcttctgc cgatggaatg ttgataatct tgttgccttt gcccttcgac
    13801 agctgcggca gatcgcttac cgggaacatc aacatacggc ctgcctgagt gattgccagc
    13861 agcatatcgg aagcatcttc aatcaccacc ggcggcataa catgggcatt ttccggtaag
    13921 gtgatcaaag ccttacctgc acggttacgc gccaccagat cgttaaaggt gcagacgaaa
    13981 ccgtaacccg catcggaagc catcagcagt ttctgatcgt cgctttccat cagcatatgg
    14041 tcaacggtcg ccccaggcgg caacgttaat ttgccggtga gcggctcgcc ctgaccacgc
    14101 gccgacggca gcgtaatcgg gtcaatggca tagctacgac cggtggaatc aacaaacact
    14161 accggttggt tgctcttacc tttcaccgcc gctttgaagc tatcacccgc tttataattc
    14221 aggcccggcg cgtcgatatc atggccttta gcgctgcgta cccagcccat ctgcgacagc
    14281 acaatggtga caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg
    14341 cgttcctgca acggcgaacg acgatcgtca gcgtaggctt gcgcgtctgc ctgcagttct
    14401 ttcttcagca ggttattcat tttacgctcg gaagccaaaa tgccctgcaa ctggtcgcgc
    14461 tctttttcca gctcactctg ctcaccgcga atcttcatct cttccagttt ggcaagatga
    14521 cgcagtttca gttcgaggat cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc
    14581 agcgccggtt tcggttcatc ttcgttacga atgatctcaa tcacttcgtc gatgttgaga
    14641 aacgccacca gcaaaccttc caggatatgc aggcgcttga ggactttctc cagacgatag
    14701 ttcagtcggc ggcgcacggt atcgcgacgg aacaccagcc attcggagag gatttccagc
    14761 aggtttttca ccgccggacg accatccaga ccgatcatat tgaggttaat gcgatagctc
    14821 ttttccagat cggtggtagc gaagaggtgg ttcatcacct gatccatatc cacgcggttg
    14881 gaacgcggca caatcaccag acgggtcggg ttctcgtggt cagattcatc acgcagatcg
    14941 tcaaccatcg gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca
    15001 cctgaaacct gatgcggtaa tgcgctgata accaccgcgc catcttcttt cttccacacc
    15061 gcgcgcatac gcactgaacc acgtccgttc tcgtagattt tacggatctc ggcgcgcgaa
    15121 gtgataattt ccgcttcagt cggataatcc ggcccctgca cgatatccag cagctgatcg
    15181 agcgtggttt tcggctggtc gattaatgcg attgccgcct gagctacttc acgcaggtta
    15241 tgcggtggaa tatcggtcgc catgccgacg gcaataccgg tggtgccgtt aagcaaaatg
    15301 tttggcagac gagcaggcag cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc
    15361 cagtcagccg tcccctgccc cagctcgctc aatagcagct cggaatattt cgacaaccgg
    15421 gattcggtgt aacgcattgc cgcgaacgat ttcggatcgt ccggcgcgcc ccagttcccc
    15481 tgaccatcaa ccagcggata acggtaagag aacggctgcg ccatcaggac catcgctcca
    15541 taacaggcga tatcgccgtg cggatggtat ttacccagta cgtcaccgac ggtacgggcc
    15601 gattttttaa atttggcgct ggcattcagg cccagttcag acatcgcata cacaatgcgg
    15661 cgctgaacag gtttcagacc atcaccaata aacggcaacg cacggtccat gatcacgtac
    15721 atggagtagt ttaagtaggc gttttccgta aattcatgta gcgcaaggcg ctctgccata
    15781 tcgctcatta attctgattc ctcaacttat tcgccagcct tcaaaggggc aatattgccg
    15841 gagatactac attatctgcc gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg
    15901 acgtgctccg acggcgataa aggcatcaat gtgccttgat atacaactct ttgctgtaga
    15961 cttgatcggc atagagggca gcacaaggtt gcgcccatgc cacaccactc ggcatggggg
    16021 tccgctccgg gcgattcgag aagttgaggt tatgcgagtc cagggaagcc taaagcatca
    16081 aagtagtcga ctgtaagtat acggttcagc ccagtttagc tgtgtctcca ccagtgcccc
    16141 gcgcctcctg ccataagtgc ggcttgctca tggatacccc cgagacggct tacggcgcac
    16201 cggataataa atttcgcgta tacatggtat tccttaccgg agaaattata agcccaacaa
    16261 caatacgcct gtggcattgt tcgctcaaac ttatcgcgag tgatttggct cacactccgg
    16321 taaagattcc ccatcacgcc aacggcacat aaacatccgt ttccagtcgc tgctctgtca
    16381 ccccttcggc aagattggtg tagtgaaaca gaatcggtgc tttacgcatt ttctcgccgc
    16441 ttgcaggcag ccagtggcga ataatgcccc atatcgtgtg tgaaatatcg tccagctcgc
    16501 caacgtggcg ggccacggca taacgtccac cggtaagttc accattgctc acaccataac
    16561 gattatcggg aatcggttcg ctaacgctgc cgcagatatc aaagcgaaac gcttccggtg
    16621 cggtggtggc agggtcgtcc caggcgacgc caaaagtctg gctttggtta acaggagaaa
    16681 gccccgtctc tttacgccac atgataaatt tcgccacact gtagttgagc aactcagggc
    16741 tacagcggtg cggcaacatc gccacaggga ttgatgggaa atcgataatg tttacatcca
    16801 gtgtcaggtt tgtcattttg cctccctgcg aaattattcc cgggaacgcg tcccgggagc
    16861 ggtaacaatt acggatttac tttgcggatc tgtttgacgt caatttcaac agaattccag
    16921 tctttatcga cttcaccctg aatctcaacc gtatctttcg gcgtcaccgt cacaccgttc
    16981 cagcgtttgt ggtcgatatc aacattgata gtaccgctgg catctttaaa cacgtagaga
    17041 tcgtcagaga tgcgttcaac gatattgccg cgcagggtta cccaggtgtc gtcacgcagg
    17101 gattttgcgc tttctacggt cgttacgctg ccgttcggcc cctggaatcc tccggcctga
    17161 ctttgcgttg ccgatgggcc agaaaaaccg ccctgctctg ctgccatcac cggtgcgctg
    17221 cacagggcca ttactgcgat tactgctgcg aattttttca tgtttattac tccctttaat
    17281 gtctgtttcc gagcatttaa caagatagtc cttaacaact tcttaaggga aaaaaataaa
    17341 atttagtgct gtacagagcg cgttacaaca cggtttactg gcagcaaata cggttatcgc
    17401 agggatgaaa aaatgcgaat tttactgata gaagatgaca tgctgattgg cgacggcatc
    17461 aaaacgggcc ttagtaaaat gggttttagc gtcgactggt ttacacaagg tcgtcaggga
    17521 aaagaggcgc tttatagcgc gccttatgat gcggtgatcc tggatttaac cttaccaggc
    17581 atggatg
//
LOCUS       DAEVZA010000104        17541 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEVZA010000104 DAEVZA010000000
VERSION     DAEVZA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.104"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..332)
                     /gene="paaD"
                     /locus_tag="LAF92_003567"
     CDS             complement(<1..332)
                     /gene="paaD"
                     /locus_tag="LAF92_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HBI9792517.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISP"
     gene            complement(347..1093)
                     /gene="paaC"
                     /locus_tag="LAF92_003568"
     CDS             complement(347..1093)
                     /gene="paaC"
                     /locus_tag="LAF92_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HBI9792518.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFIRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(1102..1389)
                     /gene="paaB"
                     /locus_tag="LAF92_003569"
     CDS             complement(1102..1389)
                     /gene="paaB"
                     /locus_tag="LAF92_003569"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HBI9792519.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(1401..2330)
                     /gene="paaA"
                     /locus_tag="LAF92_003570"
     CDS             complement(1401..2330)
                     /gene="paaA"
                     /locus_tag="LAF92_003570"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HBI9792520.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTIPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            2615..4660
                     /gene="paaZ"
                     /locus_tag="LAF92_003571"
     CDS             2615..4660
                     /gene="paaZ"
                     /locus_tag="LAF92_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HBI9792521.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVNM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFITDAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            4908..7181
                     /gene="tynA"
                     /locus_tag="LAF92_003572"
     CDS             4908..7181
                     /gene="tynA"
                     /locus_tag="LAF92_003572"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HBI9792522.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(7239..8738)
                     /gene="feaB"
                     /locus_tag="LAF92_003573"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(7239..8738)
                     /gene="feaB"
                     /locus_tag="LAF92_003573"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HBI9792523.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            8974..9879
                     /gene="feaR"
                     /locus_tag="LAF92_003574"
     CDS             8974..9879
                     /gene="feaR"
                     /locus_tag="LAF92_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HBI9792524.1"
                     /translation="MNPAMDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(10051..10380)
                     /locus_tag="LAF92_003575"
     CDS             complement(10051..10380)
                     /locus_tag="LAF92_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBI9792525.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(10385..10570)
                     /locus_tag="LAF92_003576"
     CDS             complement(10385..10570)
                     /locus_tag="LAF92_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBI9792526.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(10567..13206)
                     /locus_tag="LAF92_003577"
     CDS             complement(10567..13206)
                     /locus_tag="LAF92_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBI9792527.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMEKSQMPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAEQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            13414..14403
                     /gene="ldhA"
                     /locus_tag="LAF92_003578"
     CDS             13414..14403
                     /gene="ldhA"
                     /locus_tag="LAF92_003578"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBI9792528.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            14514..14936
                     /gene="hslJ"
                     /locus_tag="LAF92_003579"
     CDS             14514..14936
                     /gene="hslJ"
                     /locus_tag="LAF92_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBI9792529.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(14933..15199)
                     /locus_tag="LAF92_003580"
     CDS             complement(14933..15199)
                     /locus_tag="LAF92_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBI9792530.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            15473..17509
                     /gene="nifJ"
                     /locus_tag="LAF92_003581"
                     /pseudo
     CDS             15473..17509
                     /gene="nifJ"
                     /locus_tag="LAF92_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
BASE COUNT         4306 a         4581 c         4348 g         4306 t
ORIGIN      
        1 gggctaatgc catactgtcg cagacgctca cgggcatcgg gggtcatcca gtcggtggtc
       61 catgccgggt cgagttgcag cacaacctga acgggggtaa acccgtgggt tgtcattgcc
      121 tcacgtatcg cgccaatcaa atgttccgtt gccggacaac cggaataagt cggcgtaaag
      181 ccgatcaccc atccttctcc catctgtgtc acattacgca ccatgcctaa atcggtaatg
      241 gtcagcaccg ggatctccgg gtccgggatc tggctgagta gcgcccatat ctcatgaacc
      301 tgtggcggtg caatggtggc cagacgttgc atacccatct cctctgttac cattgctgac
      361 cgggcaagac acgctggaga tactgcattt ctgccagcat gggtccaagg tgttcggtat
      421 gcagtccttt tttaccgcca gtgcgatacg cctgctcttg cggtacgttc aatgtggctt
      481 cgttgatccc ggcaaaaact tcggcttccc acgctgcgcg taaagtgcgt ggatcaaccg
      541 caataccctc ttcactcagt gcaatatcaa tctcgtcggc atcgaacagt tcggcggtaa
      601 aacgccacaa cttgttgatg gcctgctgca tcttttgccc tgatacgtca gtaccattgc
      661 ccagtcgctc cagccagcca cgaataaaac gcaggtgata acgcgcttct ttaattgctt
      721 tggcagaaat cgccgccagt tgcggatcac ggctctccat cagacgggta aagagcgcca
      781 catgccatgc atcgatgaaa tactggcgtg caatggtgtc ggcaaaattg ccgtttggct
      841 gttcaaccaa caataagttg ctgaactggc gctcgtctcg ggtgaaggcc agggtatctt
      901 catcgccttc tcccgctaat tcagcggcat acgataagaa gttgcgtgcc tggcctaata
      961 aatcgagtcc aatatttgcc agtgcgagat cgatttccag ttccggtgca tgaccgcacc
     1021 attcacccaa ccgctgggag agcaccaggc agttatcgcc caggcgcaag gtgtaagccg
     1081 ttaactgatt catttccgac ctcacatgtg ctcaatgcca tcagggatgg tgtaaaacgt
     1141 tggatggcga tagaccttgc tttcagccgg atcaaaaaat tcaccgcgtt cttccggttg
     1201 cgaggcaaca atttcactcg ccttcaccac ccaaattgaa catccttcgc tacgacgggt
     1261 gtaagcatca cgggcatttt ccagtgccat ccgctcatcg gcagcatgta aactgccaac
     1321 atggcggtgt gataagccct gtttgccacg cacgaaaact tcgtataacg gccagtaaac
     1381 attactcatt ttgaatctcc ttatgcgacc ttacgggcaa gttgtttttg tgcatgggcc
     1441 agcgctgctt cccgtaccca ggtaccttct tcccaggctt tacgtttggc gtcgagcctc
     1501 tcctgattac aaattccgcg accgttaatt acttcgttaa actcctgcca gtcgatctca
     1561 ccaaagcggt agtgaccgct ttcagtgtca aaatgcagat ccgggtcagg aacggtcata
     1621 ccgagcattt caacctgtgg aatggtgtta tccacgaaac gctggcggag ttcgtcgttg
     1681 gtgaaacgtt tgattttcca ggcgagactt ctggcgctgt ttggcgagtt atcatcgttt
     1741 ggcccgaaca tcattaaggc tggccaccag aaacggttaa tggcgtcttg caacatctgc
     1801 ttttgagctt cgctgccctg cgccagtgcc atgcaggctt caaacccctg acgctggtga
     1861 aaactctctt ctttgcagat tttcaccatt gctctggcat acgggccata agaagtgcga
     1921 cataacgcca cctgattgac gatcgctgcg ccatccacca gccagccgat aacgccgata
     1981 tcagcccaac tcaatgtcgg ataattaaag atggaggaat atttcatccg tccgtcgagc
     2041 attttttggt agatgtcttc tctggcgcag cccagtgttt cggcggcgct atagagatac
     2101 agaccatgac cggcttcatc ctgcactttg gccaacagaa tggctttacg ccgcaaggtt
     2161 ggtgcgcgag tgatccagtt accttcaggc aacataccaa caatctcgga gtgcgcatgc
     2221 tgcccaatct ggcggatcag tgtctttcgg taagcatcgg gcatccagtc ctgtggctcg
     2281 atagccgttt cctgggctat ccgttgctca aagcgttctt cttgggtcac gtcatcacct
     2341 ttacgattcc agttttcgtg aattaattaa aaacacgaat cacattgttt tatgaaagtt
     2401 acacaatggt taactaaaat cacaaatttt gtatgcttga actgtgacgg atttcgcacc
     2461 ctatttttat aaatcattat ttatcagtgc attaatgttt tccgcagttg ctatacagat
     2521 cgcatagtta acatttcgtt aaaagaaccc ttgcttttta tgattcgcaa tttaactatt
     2581 agcaacataa atgtgaaaca tctggagagt agcgatgcag cagttagcca gtttcttatc
     2641 cggtacctgg cagtctggcc ggggccgtag ccgtttgatt caccacgcta ttagcggcga
     2701 ggcgttatgg gaagtgacca gtgaaggtct tgatatggcg gctgcccgcc agtttgccat
     2761 tgaaaaaggt gcccccgccc tccgcgcgat gacctttatc gaacgtgcgg cgatgcttaa
     2821 agcggtcgct aaacatctgc tgagtgaaaa agagcgcttc tatgctcttt ctgcgcaaac
     2881 aggcgcaacg cgggcagaca gttgggttga tattgaaggt ggcattggga cgttatttac
     2941 ttacgccagc ctcggtagcc gggagctgcc tgacgatacg ctgtggccgg aagatgaatt
     3001 gatcccctta tcgaaagaag gtggatttgc cgcgcgccat gtactgacct caaagtcagg
     3061 cgtggcagtg catattaacg cctttaactt cccctgctgg ggaatgctgg aaaagctggc
     3121 accaacgtgg ctgggcggaa tgccagccat catcaaacca gctaccgcga cggcccaact
     3181 gactcaggcg atggtgaaat caattgtcga tagtggtctt gttcccgaag gcgcaattag
     3241 tctgatctgc ggtagtgcgg gcgacctttt ggatcatctg gacagccagg atgtggtgac
     3301 tttcacgggg tcagcggcga ccggacagat gctgcgagtt cagccaaata tcgtcgccaa
     3361 atctatcccc ttcactatgg aagctgattc cctgaactgc tgcgtactgg gcgaagatgt
     3421 caccccggat caaccggagt ttgcgctgtt tattcgtgaa gttgtgcgtg agatgaccac
     3481 aaaagccggg caaaaatgta cggcaatccg gcggattatt gtgccgcagg cattggttaa
     3541 tgctgtcagt gatgctctgg ttgcgcgatt acagaaagtc gtggtcggtg atcctgctca
     3601 ggaaggcgtg aatatgggcg cactggtaaa tgctgagcag cgtgccgatg tgcaggaaaa
     3661 agtgaacata ttgctggctg caggatgcga gattcgcctc ggtggtcagg cggatttatc
     3721 tgctgcgggt gccttcttcc cgccaacctt attgtactgt ccgcagccgg atgaaacacc
     3781 ggcggtacat gcaacagaag cctttggccc tgtcgcaacg ctgatgccag cacaaaacca
     3841 gcgacatgct ctgcaactgg cttgtgcagg cggcggtagc cttgcgggaa cgctggtgac
     3901 ggctgatccg caaattgcgc gtcagtttat taccgacgcg gcacgtacgc atgggcgaat
     3961 tcagatcctc aatgaggaat cggcaaaaga atccaccggg catggctccc cactgccaca
     4021 actgttacat ggtgggcctg gtcgcgcagg aggcggtgaa gaattaggtg gtttacgagc
     4081 ggtgaaacat tacatgcagc gaaccgctgt tcagggtagt ccgacgatgc ttgcagctat
     4141 cagtaaacag tgggtgcgtg gtgcgaaagt cgaagaagat cgtattcatc cgttccgcaa
     4201 atattttgag gagctacaac caggcgacag cctgctgact ccccgccgca caatgacaga
     4261 ggccgatatt gttaactttg cttgcctcag cggcgatcat ttctatgcgc atatggataa
     4321 gattgctgct gccgaatcta ttttcggtga gcgtgtggtg catgggtatt ttgtgctttc
     4381 tgcggctgcg ggtctgtttg tcgatgccgg tgtcggtccg gtcattgcta attatgggct
     4441 ggaaaacttg cgctttatcg aacccgtaaa gccaggcgat accatccagg tgcgtctcac
     4501 ctgtaagcgc aagacgctga aaaaacagcg tagcgcagaa gaaaaaccaa caggtgtggt
     4561 ggaatgggcg gtagaggtat tcaatcagca tcaaaccccg gtggcgctgt attcaattct
     4621 gacgctggtg gccaggcagc acggtgattt tgtcgattaa tcggtgaatg aagggcaacg
     4681 gccaatagtt gcccttttat ttcactaagt tttgtgacgt tgtcacatta tgcatgatgt
     4741 gtacatctat tttcagggca cccactgtat gaaaagctgg cacacctgcc aaacaacctg
     4801 gcaggtgcag gcaatcccct ttgcatcagt actgataatg tgaacctgac taaaccgccc
     4861 acagagcgcg gttgctaaca agaacacaac atctgacgag gttaataatg ggaagcccct
     4921 ctctgtattc tgcccgtaaa acaaccctgg cgttggcagt cgccttaagt ttcgcctggc
     4981 aagcgccggt atttgcccac ggtggtgaag cgcatatggt gccaatggat gaaacgctta
     5041 aagaatttgg tgccgatgtg cagtgggacg actacgccca gatctttacc ctgattaaag
     5101 atggcgcgta cgtgaaagtg aagcctggtg cgcaaacagc aattgttaat ggtcaacctc
     5161 tggcactgca agtgccggta gtgatgaaag acaataaggc ctgggtttct gacaccttta
     5221 ttaacgatgt tttccagtcc ggtctggatc aaacctttca ggtagaaaag cgccctcacc
     5281 cacttaatgc gctaactgcg gacgaaatta aacaggccgt tgaaattgtt aaagcttccg
     5341 cggacttcaa acccaatacc cgttttactg agatctccct gctaccgcca gataaagaag
     5401 ctgtctgggc gtttgcgctg gaaaacaaac cggttgacca gccgcgcaaa gccgacgtca
     5461 ttatgctcga cggcaaacat atcatcgaag cggtggtgga tctgcaaaac aacaaactgc
     5521 tctcctggca acccattaaa gacgcccacg gtatggtgtt gctggatgat ttcgccagtg
     5581 tgcagaacat tattaacaac agtgaagaat ttgccgctgc cgtgaagaaa cgcggtatta
     5641 ctgatgcgaa aaaagtgatt accacgccgc tgaccgtagg ttatttcgat ggtaaagatg
     5701 gcctgaaaca agatgcccgg ttgctcaaag tcatcagcta tcttgatgtc ggtgatggca
     5761 actactgggc acatcccatc gaaaacctgg tggcggtcgt tgatttagaa cagaaaaaaa
     5821 tcgttaagat tgaagaaggt ccggtagttc cggtgccaat gaccgcacgc ccatttgata
     5881 gccgtgaccg cgttgctccg gcagttaagc ctatgcaaat cattgagcct gaaggtaaaa
     5941 attacaccat tactggcgat atgattcact ggcggaactg ggattttcac ctcagcatga
     6001 actctcgcgt cgggccgatg atctccaccg tgacttataa cgacaatggc accaaacgca
     6061 aagtcatgta cgaaggttct ctcggcggca tgattgtgcc ttacggtgat cctgatattg
     6121 gctggtactt taaagcgtat ctggactctg gtgactacgg tatgggcacg ctaacctcac
     6181 caattgctcg tggtaaagat gccccgtcta acgcagtgct ccttaatgaa accatcgccg
     6241 actacactgg cgtgccgatg gagatccctc gcgctatcgc ggtatttgaa cgttatgccg
     6301 ggccggagta taagcatcag gaaatgggcc agcccaacgt cagtaccgaa cgccgggagt
     6361 tagtggtgcg ctggatcagt acagtgggta actatgacta catttttgac tggatcttcc
     6421 atgaaaacgg cactattggc attgatgccg gtgctacggg catcgaagcg gtgaaaggtg
     6481 ttaaagcgaa aaccatgcac gatgagacgg cgaaagatga cacgcgctac ggcacgctta
     6541 tcgatcacaa tatcgtgggt actacacacc aacatattta taatttccgc ctcgatctgg
     6601 atgtagatgg cgagaataac agcctggtgg cgatggaccc agtggtaaaa ccgaatactg
     6661 ccggtggccc acgcaccagt accatgcaag ttaatcagta caacatcggc aatgaacagg
     6721 atgccgcgca gaagtttgat ccgggcacga ttcgtctgtt gagtaacccg aacaaagaga
     6781 accgcatggg caatccagtt tcctatcaaa ttattcctta tgcaggtggt actcacccgg
     6841 tagcaaaagg tgcccagttc gcgccggacg agtggatcta tcatcgttta agctttatgg
     6901 acaagcagct ttgggtaacg cgttatcatc ctggcgagcg tttcccggaa ggcaaatatc
     6961 cgaaccgttc tactcatgac accggtcttg gacaatacag taaagataac gagtcgctgg
     7021 acaacaccga cgccgttgtc tggatgacca ccggcaccac acatgtggcc cgcgccgaag
     7081 agtggccgat tatgccgacc gaatgggtac atactctgct taaaccatgg aacttctttg
     7141 acgaaacgcc aacgctaggg gcgctgaaga aagataagtg attgtttcag acaaaaaaac
     7201 ggcaccaggt gccgtttttt acttatgagc gaaccagatt aataccgtac acacaccgac
     7261 ttagtttcac accaaccgtc cagccagtcg gggccaaaat cacggcccgt tcctgactgc
     7321 ttcatcccac caaacggtaa gttagcgtca attaaggtat ggctgtttac ccacaccgtc
     7381 cctgcctgta agcgatcgct atattccaga gcctgggaga gattttgcgt ccagacactg
     7441 gcagttaagc catattccgt gtcgtttgcc agttgtaacg cctcttctcc atccgctact
     7501 cgcaccaggt ttaccaccgg accaaacacc tcttcacgag ttaagcgcaa tttagcatcg
     7561 ggatttacca ccagcgttgg cgcaacataa tacccctctc cggctggtcc attcgacccg
     7621 cgaatcagct ctgcttgctg tgcctgcgca tcgtcgagga atgaacacac tttgtcgcag
     7681 tgcgcacgag aaaccaaagg gttaatctgt gcaacaggtg acatccccgg tcccacttgc
     7741 aacgatttta ccgcctgctc aaatccacta accagcgtgt caaacaacgg cgcttcaata
     7801 taaattcgcg aactggcggc gcatacttgc ccttgattca ggaagcttcc ggtcatcaag
     7861 ccttcaataa cccattgcgg atcagcatct tttaatacaa ttgccgggtt tttaccgccc
     7921 agttccagcg ttacacgcgt taagtgatca gcagcagttc tggcaatacc ttttcccgtc
     7981 gcggttgaac cggtaaaact gattttcgca acatgaggat gtgacgtcag ggccgcgccg
     8041 catacagcac ctgacccggt gacgacatta aaaacgccat cagggatacc agcctcgctg
     8101 gccagttccg ccacgcgcaa catcgtcagt ggcgtggttt ccgaaggctt aatcacgatt
     8161 gaacagcctg ctgccagtgc tggcatcacc ttccacatac caatcatcaa cggaaagttc
     8221 catggcacaa ttcccgccac tacgccaacc ggctctttac gcgtccaggc ctgataacgc
     8281 gccccctggg gtaagggaat cgacaagtcc agcgttttac ccgcgatttt ggtcgttaac
     8341 ccggcggtat aacgcatcca gttcagcgta cagcccactt caaaagcacg ggaaatggca
     8401 attgacttgc cttgctccag ggtttccagt tgcgccagct cctcactgtg ctgttccacc
     8461 agatcggcaa aacgtagcag aatacgttca cgctctgcgg gtaatcgccc ggcccagcga
     8521 cgcgagacaa aggcccgcca ggcagacatg actgcgttat ctacatccgc ttcgttggca
     8581 tcagcagtag acgcaatttc ttgcccggtg gccggatcaa agatcgccaa ccgtttttca
     8641 ctttgtgcgg ggccaggacg accatcaata taaagaccgt gttgacgatc gagaaactgt
     8701 tggacctggc ttaatactgc tacatgcggc tctgtcatca cttttcctta ttatttaccc
     8761 agtgtgattt cagtgtacgc agcgacaaga aaagtcactt ttttcgctgt gacattcgct
     8821 ttgtcagctg tgacaagctc cgcaaatcgt gacaataacc gcttatgtct ctatgcctga
     8881 caagacttat ttcctatagt caggctcatt agcttcgttt attgcaacac aaatgcaaca
     8941 ataaaaatac atttcacaga gcgaaaacgt gccatgaacc ccgcaatgga taatgagttt
     9001 cagcaatggc tttcccaaat caatcaggta tgcggaaatt ttaccggacg cctgctgact
     9061 gagcgttaca cgggtgtact ggacacgcat tttgccaaag gactaaagct gagtaccgtg
     9121 acaaccagcg gggtgaattt atcccgcacc tggcaggagg taaaaggcag cgacgatgcc
     9181 tggttttaca ccgtttttca gcttagtggt caggcaataa tggagcagga tgagcgtcag
     9241 gtgcagattg gcgctggcga tattacgtta ctcgatgcct cacgcccctg ttcgctttac
     9301 tggcaggagt cttctaaaca gatttcatta cttttgccac gcactctgct ggaacaatat
     9361 tttccccatc aaaaacctgt ctgcgcagaa agactggacg ctgacttacc catggtgcaa
     9421 ctcagtcatc gcctgttaca ggagagcatg aataatccgg cactttctga aacagaaagt
     9481 gaagctgcgc tacaggcgat ggtgtgtctg ctgcgcccgg tacttcatca gcgggaatct
     9541 gttcaacctc gtcgtgaacg tcagtttcaa aaagtggtta cgttgataga cgataatatt
     9601 cgcgaagaga tattacgccc ggagtggata gccggagaga caggtatgtc agtacgtagt
     9661 ttgtaccgaa tgtttgccga taaaggtttg gtagtcgcgc aatatattcg taaccgtcgt
     9721 ctcgattttt gtgcagatgc gattcgccat gccgcagatg atgaaaaact ggcaggcatc
     9781 ggctttcatt ggggattttc tgaccagagt catttttcaa cggtatttaa gcaacgcttt
     9841 gggatgacgc caggcgagta tcgacgtaaa ttccgctaac taattggcgt tgtgtgtact
     9901 gcaacgccaa ttagttaaaa cagatatttg actatgcatt tttcagggcg aaaggcaatt
     9961 tgacaggagt ttttgaatat tcaccttgtt tcaggaaaat agatcaatcg ctttctcctg
    10021 tagcgttatc ccgatattgc ggttttgcta ttaaaatttt cgcacccatt taccgtttat
    10081 cccttgtaca tattcccccg gtttggcccg agctacgagc ttttgcccgg cgagtttcgc
    10141 gatatcatct acagacacat tattttgctt tgccagttgt tggtaactcg ctttacgttc
    10201 ggcattaatg tcggttacta atttctctgt ttcagcatcc gttttcagcg caaccagata
    10261 accataaaat gtttcaccta cccgcccctg agttctggct tcatccagag tcaatgccat
    10321 tacattgctg cttaccagcc caacaagaaa cgcacaaaga agtaatgttt tcttcatcat
    10381 cgcctcagaa aagatcgcta cgagtttcaa gcagctcttc gacatctttg tctgccttga
    10441 tgatgatctc atgctcaatt ttaacgttca tattgatagt gatcggttcc ttgggcgcag
    10501 cgacttcaat acgcggcgta caggcagcca gcataaaaga tgacgtcaac gcagccagta
    10561 aaattttcat tgtttttcct cacactcttt tccttgcgga cagtcatttc ccggcagacg
    10621 tgcgttctgc tccaaccatg cctggagatt atcgccaaag cgtaaactgc gccacagcgt
    10681 aaaaatattc tcttcatgat agtaattaag gtttaccgta ccgcttttac catcaacgcg
    10741 actggtacct gtaatgttgg cctgcatggt taataaaccg agattatcta agttaatttt
    10801 tgtcgatgac cggctaattt ccatatagcg caaccagtta attgctgaac ccgcagtcat
    10861 attgtctttc accaccgcat ctgcggtgtc tttatccagc cgcaaggtca ttggcccgct
    10921 atttgccagc caaccatctt tcactatcca tttttcgttg tttagccata acggcaatgc
    10981 accactaaat gccccggaca tggcgaattg tttcggattg acggcgctaa ccagttcgct
    11041 ggatgagaga ttattcagcc gcaacagcgc cgggtcatgt tgcggcatac gtaattgttt
    11101 catcagtacg ttaccgccta acacatcgac gctaacatca gtcaacagca agggttcttc
    11161 ttcagtccac ggataacgcc cttgcaaatc agccgtaata tttttcgctg tcaccagatt
    11221 aatcacttcg gcaattcgca acgtaacggg gccgcgagtc cccagatgcc aggctccatc
    11281 ggcaaaacgg aaaggcagga caaaatcgac accgttaacc tgattatctg gcatccaggc
    11341 actaccgcct ttcaacacgc cgtgtcctcc cgcgcggaat ccttgttcag gcgcagcaga
    11401 aaatgcaacc tgagcataca gttcaccatc gcgtaagttc atcttccagt cgggtggcac
    11461 cagcggctgg aatacagtca gtgattgttt tggccaccag gcgttaccgc gcagacgaat
    11521 accgtcccag cgaccattta cccgaaccgg gccaatctca ccagcatgta aatcgccttt
    11581 aaagaggaaa tacgtcggat cgcgcccatc gacgctaaat tttaaggttg atggcggtaa
    11641 cacactgccg ccagtgaaca gcgtttgctc ggcgtccagt gacagcgcgc cgctgaagga
    11701 gggatgctgt gcgtcacgta cccagacgac gggcttgtcg agaattaatc gcggcttttc
    11761 taccgtcatc gtaccgtatt gtaactggtc gaaaccggtg gagagatcgg tcagcgtaat
    11821 cgtgctgtca tgccactcac cttttcctgc gacatcccag gtggcattca tcggtgtaaa
    11881 actgcctttt ccccagtagc gccactgcca gcggccagcg tcagggagaa aatcattcgc
    11941 cagcccatcc atatgcagca cgaaatcgcc cagttcattt tcatgcgcct gcaaaatggc
    12001 ctgcaaacgt ccgtcaacac cacgttgggt gacttttaca cccgctaaag gccagcggat
    12061 ttcatcgatg tccagcgaat cgatgactct tcccttcgaa cgtaataacg cgccgggctc
    12121 aaaggccagc gttgggtcag tcagacttcc acttagctgc gcgggtaagc gggcatataa
    12181 aatgaggtcc gcctgtttcg cttcaccggt cagttgcagt ggcatctgac ttttttccat
    12241 gcttaatttt cctggaccaa aattaagcac cgcgttgcct ttacccgctt gcccctgggt
    12301 cagcacactc agtcgcccgc tgaccagagc gttctcgagc cctgcctgcc agttgtcgac
    12361 tttgacaccc agtcggccac tcaaaggaaa acctgcatac ggccagctcc agcgaccatc
    12421 gcttacggtc aattgttgac gagtaatttg ccacggcaaa tcgagcaacg gatcgccgtt
    12481 atcccgtgcc agcacaatca attgcccgct attttcctgc cagtccagct ctgcatccac
    12541 cagtgacggt tcctgcggca agtttaacgt cgcagtagca tgcccactta caggaagtcc
    12601 atccggcacg agcggcatag taaattcccc caccagttta accggcggct gattttcaaa
    12661 cgcgacgaca tccagttcgc tgactgtaag ttgttgccct ttcagctggc cttgaaattt
    12721 aactttttcg ccctgataac gcagttgctg gatatcagag gttaatgcga gagagagttt
    12781 tccctgccat tcctgccagg gagaaaaaat cagtttatcg atattgatcc aggtgttagg
    12841 cagcatggac tgccactgcg cgagggtttt tggagcagct ggcgattgct ccgtctgcgg
    12901 caatttcgcc agacaagcag aatcaagttc taccatgccg acgttcaata accagcggct
    12961 gggatgtgaa aggctggcgt tggtgatatg cgcaagctga caatctccca ccagataacg
    13021 gagatcggga atgattaaac ctttacgcgt aatgcgtggg ctttcatcta atgcaatacg
    13081 tgtcccgagc ggtagccaga tgcccgccag cgtgggaacc cacagcccga gcgtcatcag
    13141 cagcgtcaac ggcacaagaa taatcagtaa taacagcgcg agaacggctt tatatttacc
    13201 cagcatgggt agttaatatc ctgatttagc gaaaaattaa gcattcaata cgggtattgt
    13261 ggcatgttta accgttcagt tgaaggttgc gcctacacta agcatagttg ttgatgaatt
    13321 tttcaatatc gccatagctt tcaattatat ttgaaatttt gtaaaatatt tttagtagct
    13381 taaatgtgat tcaacatcac tggagaaagt cttatgaaac tcgccgttta tagcacaaaa
    13441 cagtacgaca agaagtacct gcaacaggtg aacgagtcct ttggctttga gctggaattt
    13501 tttgactttc tgctgacgga aaaaaccgct aaaactgcca atggctgcga agcggtatgt
    13561 attttcgtaa acgatgacgg cagccgcccg gtgctggaag agctgaaaaa gcacggcgtt
    13621 aaatatatcg ccctgcgctg tgccggtttc aataacgtcg accttgacgc ggcaaaagaa
    13681 ctggggctga aagtagtccg tgttccagcc tatgatccag aggccgttgc tgaacacgcc
    13741 atcggtatga tgatgacgct gaaccgccgt attcaccgcg cgtatcagcg tacccgtgac
    13801 gctaacttct ctctggaagg tctgaccggc tttactatgt atggcaaaac ggcaggcgtt
    13861 atcggtaccg gtaaaatcgg tgtggcgatg ctgcgcattc tgaaaggttt tggtatgcgt
    13921 ctgctggcgt tcgatccgta tccaagtgcg gcggcgctgg aactcggtgt ggagtatgtc
    13981 gatctgccaa ccctgttctc tgaatcagac gttatctctc tgcactgccc gctgacaccg
    14041 gaaaactacc atctgttgaa cgaagccgcc ttcgatcaga tgaaaaatgg cgtgatgatc
    14101 gtcaatacca gtcgcggtgc attgattgat tctcaggcgg caattgaagc gctgaaaaat
    14161 cagaaaattg gttcgttggg tatggacgta tatgagaacg aacgcgatct gttctttgaa
    14221 gataaatcca acgacgtaat tcaggatgac gtattccgtc gcctgtctgc ctgccacaac
    14281 gtgctgttta ccgggcacca ggcattcctg acagcagaag ctctgaccag tatttctcag
    14341 actacgctgc aaaacttaag caatctggaa aaaggcgaaa cctgcccgaa cgaactggtt
    14401 taatcttgcc gctcccctgc attccagggg agcttattca gataatcccc aaagaccttt
    14461 catcctctat tcttaaaata gccctgagtc agaaactgta attgagaacc acaatgaaga
    14521 aagtagccgc gcttgttgcg ctaagcctgc tgatggcggg atgtgtaagt agtgacaaaa
    14581 ttgctgtaac gccagaacag ttacagcatc atcgctttgt gctggaaagc gtaaacggta
    14641 agcccgtgac cagcgataaa aatccgccag aaatcagctt tggtgaaaaa atgatgattt
    14701 ccggcagcat gtgtaaccgc tttagcggtg aaggcaaact gtctaatggt gaactgacag
    14761 ccaaagggct ggcaatgacc cgtatgatgt gcgctaaccc gcagcttaat gaactcgata
    14821 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
    14881 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
    14941 tgccacagct cccggcggca agtgactgtt cactacagcg tttgccgttg ggtaatgcac
    15001 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
    15061 cacaatttgc ttctccacta ctggacatcg acgcttttaa acctggcgct acgtgcgccg
    15121 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
    15181 cccaaaacgc tgctcgcatc ttttcttcct ctgatcttca agccaaacga caccgccata
    15241 aataataggc agcacagagg gcgacgtcga gagctgtcct gcgcgttgcc ccgcattttt
    15301 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta cgctgatgtg
    15361 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
    15421 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
    15481 tattgacggt aatggcgcgg ttgcttcggt ggcgtttcgc accagtgaag ttatcgccat
    15541 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
    15601 cttaaagaac gtttggggag acacaccacg cgtagttgaa atgcagtcgg aagcgggtgc
    15661 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
    15721 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
    15781 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
    15841 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
    15901 ggaagcgcaa gattttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
    15961 tattcatttc tttgatggtt tccgcacgtc ccacgaaatc aataaaattg tcccgctggc
    16021 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
    16081 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
    16141 gtctcgcgaa gccaccaacc catggtacga cgcggtctat gaccatgttg aacaggcgat
    16201 gaatgatttc tctgccgcga caggtcgtca gtatcagccg tttgaatatt acgggcatcc
    16261 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
    16321 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgcc tgtaccgtcc
    16381 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
    16441 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg taatgaccgc
    16501 actggcagaa gcctttaata atggcgagcg cgaaactctg ccccgtgtca ttggtgggcg
    16561 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttaccgagct
    16621 caacgcggct aaaccgaaag cgcgctttac ggttggtatt tacgatgatg tgaccaatct
    16681 gtcactgccg ttgccggaaa acaccctgcc aaactcggcg aaactggaag ccttgtttta
    16741 tggccttggt agtgatggca gcgtttccgc gaccaaaaac aatatcaaga ttatcggtaa
    16801 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaagg cgggcggcct
    16861 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
    16921 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
    16981 tttaaaacct ggcggcattt tcctgctcaa cacgccgtac agcgcagatg aagtgtggtc
    17041 gcgcttgccg caggaagttc aggccgtgct aaatcagaaa aaagcgcgct tctatgtgat
    17101 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
    17161 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
    17221 gggtgcgatt gccaaaagtt acagtagcaa aggccaggat ctggtggaac gcaactggca
    17281 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
    17341 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
    17401 cgcagcgatg ctcgcagggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
    17461 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaat agatccccat
    17521 ctggaaagag gaactctgta c
//
LOCUS       DAEVZA010000105        17213 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEVZA010000105 DAEVZA010000000
VERSION     DAEVZA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.105"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..68
                     /locus_tag="LAF92_003582"
     ncRNA           <1..68
                     /ncRNA_class="other"
                     /locus_tag="LAF92_003582"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            115..246
                     /locus_tag="LAF92_003583"
     ncRNA           115..246
                     /ncRNA_class="other"
                     /locus_tag="LAF92_003583"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(430..1107)
                     /gene="narI"
                     /locus_tag="LAF92_003584"
     CDS             complement(430..1107)
                     /gene="narI"
                     /locus_tag="LAF92_003584"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBI9792531.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1107..1817)
                     /gene="narJ"
                     /locus_tag="LAF92_003585"
     CDS             complement(1107..1817)
                     /gene="narJ"
                     /locus_tag="LAF92_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBI9792532.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1814..3352)
                     /gene="narH"
                     /locus_tag="LAF92_003586"
     CDS             complement(1814..3352)
                     /gene="narH"
                     /locus_tag="LAF92_003586"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBI9792533.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3349..7092)
                     /locus_tag="LAF92_003587"
     CDS             complement(3349..7092)
                     /locus_tag="LAF92_003587"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBI9792534.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7608..8999)
                     /gene="narK"
                     /locus_tag="LAF92_003588"
     CDS             complement(7608..8999)
                     /gene="narK"
                     /locus_tag="LAF92_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBI9792535.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9338..11134
                     /gene="narX"
                     /locus_tag="LAF92_003589"
     CDS             9338..11134
                     /gene="narX"
                     /locus_tag="LAF92_003589"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBI9792536.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            11127..11777
                     /gene="narL"
                     /locus_tag="LAF92_003590"
     CDS             11127..11777
                     /gene="narL"
                     /locus_tag="LAF92_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBI9792537.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11778..13172)
                     /gene="ychO"
                     /locus_tag="LAF92_003591"
     CDS             complement(11778..13172)
                     /gene="ychO"
                     /locus_tag="LAF92_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBI9792538.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13357..13710
                     /gene="ychN"
                     /locus_tag="LAF92_003592"
     CDS             13357..13710
                     /gene="ychN"
                     /locus_tag="LAF92_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBI9792539.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13754..14449)
                     /gene="chaC"
                     /locus_tag="LAF92_003593"
     CDS             complement(13754..14449)
                     /gene="chaC"
                     /locus_tag="LAF92_003593"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBI9792540.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14607..14837)
                     /gene="chaB"
                     /locus_tag="LAF92_003594"
     CDS             complement(14607..14837)
                     /gene="chaB"
                     /locus_tag="LAF92_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBI9792541.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15107..16207
                     /gene="chaA"
                     /locus_tag="LAF92_003595"
     CDS             15107..16207
                     /gene="chaA"
                     /locus_tag="LAF92_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBI9792542.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16612..16719
                     /locus_tag="LAF92_003596"
     CDS             16612..16719
                     /locus_tag="LAF92_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI9792543.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            17148..>17213
                     /locus_tag="LAF92_003597"
     CDS             17148..>17213
                     /locus_tag="LAF92_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI9792544.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGI"
BASE COUNT         4199 a         4588 c         4319 g         4107 t
ORIGIN      
        1 agcgttgccg caggcaacga cccgaagggc gaagcgcgca gcgctgagta atccttcccc
       61 caccaccatc actttcaaaa gtccgtgaac tctcaaacga atccgcaatt aaatattctg
      121 cccaagcggg gaaggatgag aagcttcgac caaggttcga cttgagcacc agcgagagag
      181 cgttgccgca ggcaacgacc cgaagggcga agcgcgcagc gctgagtaat ccttccccca
      241 ccaccatcgc ttccttaaat aatcaaaacc tgttactccg agctaatttt gcccgatggc
      301 accacgttta ccgggcctac aatatcctcc cctgtaggtc ggataagcga agcgcatccg
      361 acaactcacc ccgcaccgtg cccataaaaa aaccccgccg aagcagggtc tatgtgaact
      421 aaaattcgct tagtgacgag cgcgcaccag ctggtactta cgtgtcagat actccaccgg
      481 tacgctccag atgtgtacca gacgcgagaa cgggaacagc aggaataacg tcatccccag
      541 caccaggtgc agacggaaga taaacgccac accatcgagg tgttgagaag cgccaccgtg
      601 gaaggtcacc accgactgcg cccagccaac cagtttcatc atctcgctac cgtccatatg
      661 ctgagcggag aacggaatgg tcaacaggcc cagcgcgcac tggataacga gcagcgacag
      721 gatcaggata tccgctccgg tagtggttgc acgcacgcgt gggctgaaca gacgacgctt
      781 cagcaacagc acgccgccaa tcagacacag cacgccgctg gcaccaccag caaacatcgc
      841 cattttctgt ttcacttcaa tcggcagcca ggcttcatac atccagtgcg gcgtcagcat
      901 accgaagaag tgaccaacaa aaatccccag aataccgata tggaacaggt tcgacgccag
      961 gttcatccct ttgcgatcca gcatctggct ggacgccgcg cgccaggtgt actgcccgta
     1021 gtcataacgc agccagctac caatcaggaa gaccgccccg gctatgtacg ggtagatatc
     1081 aaagaagaac atattcagga attgcattag tgctgtcctc cggtggtgat attcagatat
     1141 tgcggcgcga cggcaccggc aaagcgacgc tgatgagcag tgattgctga atcaccgcag
     1201 cctttgtcag caaagaattt aacctgctct tcttcccaga cagcatccag cgcctgcggc
     1261 gtatcatcgc gcgcttcgtc ggcaattttt tccgccactt tgtcgctgtc gatagcggta
     1321 ttggccaatt tcagcagcag atcaaacatc acggcataac ggctttcacg ctgttgcaga
     1381 cgcgcgctca gcaatgccag aatcggcgcg atatctttca aaccttccac ggcttcgctt
     1441 tgcggcagct gcgacaggta ctccagatac agcggcagat ggtccggtaa ttcgcggctg
     1501 tttaactgca agccgtgctg ctcgtactgc gccagcaggt ccaccatcgc ctggccgcgg
     1561 tcacgggatt caccgtgaac atgttcaaat agcaacagtg acgtggcgcg gccacggtcg
     1621 aacagttcgc tgtactgggc ctgggcatcg agcggatcca tcgccgttaa atcgcgcagg
     1681 aaaatgccca gcgcatgggc atcttctttc gacagatttt tcgacgcagc aatcgcctca
     1741 aacatctctt gttgatgctg ccataaggca gcatccggat attcaaggag acgcgataca
     1801 atcacgagtt cgatcatgga tgcggctccg ttttgctggt cacatcgatg gcatcgatac
     1861 gacggctgtt gaacagattg aatttggtat ctgaaccgtg gcagccatca ccaaaggtaa
     1921 agccgcagcc atttttctcc gggaaggctt cccgtgccag ttcacgatga ctactcggca
     1981 ccacaaagcg atcttcgtag ttagcaatcg ccagataacg gtacatctcc tgtgcctggg
     2041 cttcggtcag accgacctct tccagcgcac gggtatcaac tttaccgtca acggtttcag
     2101 cacgtttgta atgacgcatc gccagcatac gtttcagtgc gcgcagtacc ggtttggtat
     2161 caccggcggt cagcagattc gccagatact gtaccggaat acgcaagctt tcgacgtctg
     2221 gcagaatgcc gttgctaccc agctcacccg cgtctgccgc agactgaatc ggagacagag
     2281 gcggcacgta ccagaccatc ggcagtgtgc gatattctgg atgcagcggc agcgccagtt
     2341 tccattccat tgccatttta taaaccggcg actgctgtgc ggcttcaata acgctcagcg
     2401 gaataccgtc tttaatcgcc tgctcgatga ctttcggatc gttcggatcg aggaacacct
     2461 ccagctgacg ctggtaaaga tctttctcgt tctcggtgct ggctgcacgt tcaatcgcgt
     2521 cggcatcgta cagcagcacg ccaagataac ggatacgacc gacacaggtt tctgagcaca
     2581 cggtcggctg acccgcttca atacgcggat agcagaagat gcacttctca gacttaccgc
     2641 tcttccagtt gaagtagatt tttttgtacg ggcatccggt gatgcacata cgccagccac
     2701 ggcatttatc ctggtcgatc aggacgatgc catcttcttc acgcttgtaa atcgcaccgc
     2761 tcgggcaggt cgccacacat gccgggttca ggcagtgttc gcacaggcgc ggcaaataca
     2821 tcataaaggt gttttcgaac tggctataca tcgccttctg gatgttgtcg aagttcttgt
     2881 ctttcgccag tttgtcaaac tcaccaccca ggtcatcttc ccagttcggc cctttttcga
     2941 ttttcgccat ccgttccccg gtaatcagcg aacgcggacg ggcaatcggc tgcgatttgc
     3001 tgccttccgg cgcggtatgc aggttctgat agtcaaaatc gaacggctcg taataatcgt
     3061 cgatccccgg cagatgcggg ttagcgaaga ttttacccag caacatggca cggttaccca
     3121 tgcgcggctg cagtttgccg ttgattttac ggatccagcc gcctttgtat ttttcctggt
     3181 tttcccagtc agtcgggaag ccctgacccg gcttggtttc cacgttgttg aaccacgcgt
     3241 attccacgcc ttcacggctg gtccagacgt ttttacaggt aactgaacag gtgtggcagc
     3301 cgatgcactt atcgagattc agcaccatgc cgacttgtga acgaattttc attttacgct
     3361 ctcctgtacc tggtcattgc cttcgccatc taaccagtca atgttcttca tcttacgcac
     3421 tacaacaaac tcatcgcggt tagaacctac ggtgccgtag tagttaaagc cgtatgccag
     3481 atgggcatag ccgccgatca tatgcgtcgg tttcggcgtg atacgggtga ccgagttatg
     3541 gataccacca cgctgttggg taatttccga accaggcagg ttaacgatac gttcctgcgc
     3601 gtggtacatc atggtcatcc ctgccggaac acgctggctg acaacagcac gggcagtcag
     3661 agcaccgttg ctgttgaata cttcaatcca gtcgttatcg gcgataccca gatctttggc
     3721 atcggcttca ctcaaccaga ccaccggacc accgcgacct aaagtcagca tcagcaggtt
     3781 gtcgctgtag gtggagtgga taccccactt ctggtgcggc gtcaggaagt tgagcgcttt
     3841 ttccgggttg ccgttggatt tctggcctat cacttctttc accgaacggg tgtcgatcgg
     3901 cggacgataa accagcaggc tttcaccgaa atcacgcatc cactggtgat cctgatacag
     3961 ttgctgacga ccagagagcg tacgccatgg gatcagctcg tgaacgttgg tgtaaccggc
     4021 gttgtaagaa acgtgttcat cttccagacc agaccaggtc gggctggaga taattttgcg
     4081 cggctgtgcc tgaatatcgc ggaagcggat cttctcgtct tctttattca gcgccagatg
     4141 cgtatggtca cgaccggtaa attcgctcag ggcagcccag gctttcacgg ctacctgacc
     4201 gttggtttcc ggtgccagtg tcaggatcat ctctgccgca tcaattgcgg tgttcagcat
     4261 cggctggcct ttcgccggac cttccgcttt ggtgtagttg agcttacgca gcagatccat
     4321 ctcgctctgg gtgttccagg caatcccttt accgccatta ccgattttct ccatcagcgg
     4381 gccgatagag gtaaagcgtt cgtaagtcgc cggataatcg cgctctacga ccataatgtg
     4441 tggcgcggtt ttacctggga tcaggtcgca ctcgcctttt ttccagtctt tcacatccag
     4501 cggctgcgcc agttcagcgg cagagtcatg ctggataggc agcgtgacga tgtcggtttc
     4561 tttacccaga tggccgacgc acacttcgga gaatttcttc gcgatggctt tgtagatttc
     4621 ccagtcgctt ttcgcttccc aggccggatc gaccgccgca gacagcgggt gaataaacgg
     4681 atgcatatcc gaagtattca tgtcgtcttt ttcgtaccag gtcgccgtcg gcaaaatgat
     4741 gtcggaatag agacaggtgc tcgacagacg gaagtccagc gtaaccacca gatccagctt
     4801 gccttccaga ccattgtcct gccagtccac ttcttccggc ttcacgccgc cctgctgccc
     4861 cagatcttta ccctggatac cgtgctccgt ccccagcagg tacttgagca taaactcatg
     4921 gcctttaccg gaagaaccga gcaggttaga acgccagatg aacaggttgc gcgggtggtt
     4981 tttaccgttt tctggttgtt ctgccgcaaa acggatggaa ccctctttaa gggatttcac
     5041 ggtgtaatct accggattca tcccggcttt ttctgcttcg ccagcgatag tcagcgggtt
     5101 agtgcctaac tgcggcgcag acggcagcca gcccatgcgt tctgcacgga cgttaaagtc
     5161 gatcaggtgt ccggtataac gggatttatc cgccatcggc gacagcagtt cttccgcagt
     5221 cacggtttca taacgccact ggctggagtg attatagaaa taagaagtgc tgttcatatg
     5281 acgcgccgga cgctgccagt caagggcaaa cgccagcggc tgccagccgg tttgcggacg
     5341 cagtttttcc tgacctacat agtgcgccca gccgcccccg ctctgaccga cacagccgca
     5401 gaaaatcagc atgttgatca gaccgcgata gttcatatcg aggtgatacc agtggttcag
     5461 ccccgcaccg acgataatca tcgaacgacc gtgcgtttta tcagcgttat cggcaaattc
     5521 acgggcgatg cgaataatct ggctgcgaga aacgccggtg atctgctcag cccaggccgg
     5581 ggtgtaagct ttcacatcgt catagctggt tgcacagtta acgtcgttca ggccacgttc
     5641 cagaccgtag tttgccagcg tcagatcata aacggtggtc accagggcgg tgctgccatc
     5701 agccagttgc aggcgtttca ccggcagttt gtgcagcagc acgttttcca gttccacttt
     5761 gttgaagtgt tccgtgccgt cgccaccaaa gtacgggaag cctacctcag cgatctcatc
     5821 ctggctaccc agcaggctca gttgcagctc ggtttcttcg ccagttttgc cgtcgcgctg
     5881 ctcaagattc catttgccct tctcgcccca gcggaagcca atagaaccgt tcggcgcaac
     5941 catttcgcca ttggtattaa aggcgacagt tttccattcc ggattgtttt cctggcccag
     6001 cgcatcaacc agatcagcag cgcgcagcat acgacctgca gcgtagtaac cgtcgcgttc
     6061 ttccagcatc accagcatcg gcatgtcggt gtagcgacgc acatagtcgg tgaaatactg
     6121 gcttgggttg tcgaggtgga attcacgcag cattacgtgg cccatcgcca gcgccattgc
     6181 cgcatcggtg ccctgtttcg gtgccagcca cagatcgcac agtttggcga tttcagcgta
     6241 gtctggtgtg acggcaacag ttttggtccc tttgtaacgc acttcagtaa agaagtgagc
     6301 atccggggta cgcgtctgcg gtacgtttga cccccaggcg atgatgtagc tggagttgta
     6361 ccagtcagca gattccggta cgtcagtttg ctcgccccag gtttgcggag acgcaggagg
     6421 caagtcgcag taccagtcgt agaagcttaa gcaagtaccg ccaatcagcg agagatagcg
     6481 tgcacccgat gcgtaagaaa ccatcgacat tgccggaatt ggcgagaaac cagcaacacg
     6541 gtccgggccg tagtttttga tggtgtaaac gttagatgcg gcgatcagtt cgttcacctc
     6601 ctgccaggaa gaacgaacaa atccaccgcg tccacgcgcc tgcttaaagc ttttcgcttt
     6661 atcggcgtct tcaatgatag aagcccatgc ctcaaccgga tcgctatgca gcgccttcgc
     6721 ttcacgccac attttcatca ggcgtttgcg catcatcggg tatttcaggc ggttggcgct
     6781 gtaaagatac caggagtagc tggcaccgcg cgggcagccg cgaggttcat ggtttggcag
     6841 atccggacgg gtacgcggat agtcagtctg ctgggtttcc caggtgacca gaccgttttt
     6901 gacgtagatt ttccagctgc aggagccggt gcagtttacc ccgtgggtag agcggacgat
     6961 tttgtcatgc tgccaacgct ggcgatatcc atcctcccag tcacggttgg tattgagaag
     7021 ctggccatgc ccatcggcaa aggtttcacc cttctgtttg aagtagcgaa accggtccag
     7081 gaatttactc atcggttttc tcctgtggga gcctgtcggc cctctgataa atcgacattg
     7141 ctaatgttta agggcaaggt aacgctctga aacagcgtgg gaattgataa cgatcaagtg
     7201 tgatggggat gaaaaataaa gtaaattcct aagatacccc tttaggataa ttaacagtta
     7261 aagcataaca tgctgtaaat taagacgttt tatacctgta gttatttaaa gcatgaagat
     7321 ttttatgcag atgggtatta aggagtattc cccatgggta acatattaat ggctcttaat
     7381 gaaatccggc gaaataagcc atataacatt agtatgttgt aactaaataa cgcataaaaa
     7441 aagcgcgaca tcatgccgcg caaaggataa tcaaatattt tactctatat aattcggttt
     7501 acaggaaggc ggcaagtgaa tgaatcccag gagcttacta aagtaagtga ctggggtgaa
     7561 tgaacgcagc cgcagcacat gtaacccgaa gtatgacgag taacggttta ttttttagaa
     7621 tgccgaccat ataccgccca ggtaatcact acgcaggcga tatagaaaat caaaaatacc
     7681 ttcattgcgc cgactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
     7741 aagaagccac caatagcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgtcggtt
     7801 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
     7861 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
     7921 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
     7981 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
     8041 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
     8101 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
     8161 agcatcgcaa agcccgcgga gaagccgata aaggagccga aggttgccag atacagcagg
     8221 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
     8281 gaggtagcaa gatcgttcat gccaaaccac gctgcaatgg tgaagatggc aaggaacggc
     8341 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
     8401 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
     8461 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
     8521 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
     8581 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
     8641 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
     8701 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
     8761 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
     8821 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
     8881 atccacaggt tacggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
     8941 ggtcgccaat ctgtaatgac agctccagta gccctttcgg gggcggatga gtgactcata
     9001 gacacctctg atactcgttt cgatgtctgc caccttagtg tctgtagcta aaggcaattt
     9061 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
     9121 gagtacagtg atttcataaa aattatgaga tttttcacag tgctgtaaaa tccctaccct
     9181 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc gggtatgggt
     9241 atacttcagc caatagccga gaatactgcc attccagaat gtatcgtcac attcattaag
     9301 gttattgctc atttaaagcc tgaaggaaga ggtttacatg cttaaacgtt gtctctctcc
     9361 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
     9421 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
     9481 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcagcagtgc cattaagcga
     9541 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
     9601 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
     9661 tgaactgatc cctgcgttga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
     9721 ccagtttgtt gccgggcttg atcaactggt atctggtttt gaccgcacca cggaaatgcg
     9781 catcgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
     9841 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
     9901 ggcgagtgcc gtcagtcatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
     9961 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    10021 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    10081 ctctttttta tggcaggcta accgccgttt gcattcccaa gccccgctgt gtgaacgcct
    10141 gtcacctgta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    10201 ttatgatact gatgatgaag agaatcatca ggagtttacc tgccaaccag atatgacttg
    10261 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    10321 aaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    10381 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    10441 actcaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    10501 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    10561 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    10621 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    10681 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaggcgag
    10741 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    10801 tcgcctggtg ccttcgcatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    10861 taacgccctc aaacattcgc aagcgagtga agtcgtggtg acggtggcgc aaaacgataa
    10921 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    10981 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    11041 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    11101 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    11161 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    11221 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    11281 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    11341 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    11401 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    11461 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    11521 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    11581 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    11641 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    11701 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    11761 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    11821 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    11881 accaccaccg acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    11941 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    12001 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    12061 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    12121 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    12181 ccacgtaacg actgactttc ggcaacctca cccgcagaaa gttgtttttt gagcggtaca
    12241 ccaaagcggt aattaagatt cagcccgagg ttattttgat tctcgccact ttcaccctgt
    12301 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    12361 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    12421 tgttctacgc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgggctgtc
    12481 aggtcatacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    12541 aacggctgat aaaagtttgc cgatagtcgc aaatattcgc cccacgcttc ggcaccaaag
    12601 cccgctcgct gaagattttc gtccagcaag ttgtcataaa aagtgttata acccaccagc
    12661 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc atcatcctgc
    12721 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    12781 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    12841 tttccccatg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    12901 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    12961 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    13021 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    13081 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    13141 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    13201 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    13261 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    13321 gatgaatgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    13381 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    13441 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    13501 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    13561 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    13621 acacttcctc ttattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    13681 ctgtcagccg ataaagtgct cacattttaa tgtttgaggg ggcgtctgac gccccactat
    13741 gagggaagtt tactcaggcg aatcccggcc gtaacacacc atccggataa ttctccgcca
    13801 gcagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    13861 taagctcctg ttccagtgaa aacaggtatt gtgcgttggt tcccagcgga ccgctcgccg
    13921 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    13981 ggtccataat aaacacaatg gcgtttactg tgcatccatc atcaagatca agctgacacc
    14041 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    14101 ccagcgtctc ttctggcagt cgataggcga cgcctgtagt gcgtccgccc tctttcagtg
    14161 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    14221 cgcgatgcca tccaaccagt gtaccggtgc acgactcggt aaactccagt gccggattcc
    14281 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    14341 tcgccgccag cgaagccgcc cgttgctctg ctgaccataa gagtgattct tcaatggcac
    14401 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    14461 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt tactgctcat
    14521 tttgaaacca ctaacgggac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    14581 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    14641 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    14701 gcgtcatcac gccgatcgtc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    14761 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    14821 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    14881 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    14941 aacaaacatt gtctaaacgt aatacttatt tttatgtccc aatgagtaaa aaatcgacag
    15001 tggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    15061 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    15121 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggcgctggt
    15181 agtgctgttc ctgtggggaa gcagccagac actaccagtg gtcattgcca tcaatcttct
    15241 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    15301 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    15361 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    15421 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    15481 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    15541 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    15601 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    15661 tttgctgatc cagaccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    15721 tgatgacgat gacccgcatc acggtaaacc gtctgcccat agcagcgtgt ggcatgctat
    15781 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaatg ccagcccgct
    15841 ggagacattg ctcgacagca tgaatgcccc tgtcgccttt actggcttcc tggtggctct
    15901 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggtcca
    15961 gcgcgcgatg aatctgttct ttggttcagt gttagcaacc atttcgctta ccgtacctgt
    16021 cgtcacgcta attgccttta tgacgggtaa cgaattacag tttgcacttg gtgcgccaga
    16081 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    16141 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    16201 tgcctgacag ttatatctcc tccgatgacg gaaatcgggg gagataagat aaattcctgc
    16261 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    16321 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    16381 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    16441 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    16501 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    16561 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    16621 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    16681 accgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    16741 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggccattttt
    16801 gtctttatct cttccacaag taaatattgg caacccgagc tggaagcatc atccagagta
    16861 ctcatgaaac gactgtaaca gctgttgaaa aaccggaatg cactcttcat tgcaaacatt
    16921 tcagggatga agtgaagatg aattgagacg tagtatccac accaagccgg aattatccgg
    16981 tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga aagagaaaac
    17041 ccccgcacgt tgagaggtaa aacctgacaa cagaacgggg gctaatcttg aaccatcaac
    17101 aattcaagaa tagccgcaaa atattgtcat tacaacaagg cggctatatg acgctcgcga
    17161 agtttgccat gattttctgg cacgacctgg cagcaccgat cctggcgggg att
//
LOCUS       DAEVZA010000106        16870 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEVZA010000106 DAEVZA010000000
VERSION     DAEVZA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.106"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1334..1903
                     /locus_tag="LAF92_003598"
     CDS             1334..1903
                     /locus_tag="LAF92_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBI9792545.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            2163..2564
                     /locus_tag="LAF92_003599"
     CDS             2163..2564
                     /locus_tag="LAF92_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792546.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            2552..2959
                     /locus_tag="LAF92_003600"
     CDS             2552..2959
                     /locus_tag="LAF92_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBI9792547.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDENYEGEKCCPSCGTGVYSREGGQEGNWKDRN"
     gene            3243..4862
                     /locus_tag="LAF92_003601"
                     /pseudo
     CDS             3243..4862
                     /locus_tag="LAF92_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            4942..5234
                     /locus_tag="LAF92_003602"
                     /pseudo
     CDS             4942..5234
                     /locus_tag="LAF92_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(5274..6308)
                     /locus_tag="LAF92_003603"
     CDS             complement(5274..6308)
                     /locus_tag="LAF92_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HBI9792548.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQ
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(6311..7276)
                     /locus_tag="LAF92_003604"
     CDS             complement(6311..7276)
                     /locus_tag="LAF92_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI9792549.1"
                     /translation="MKKNKYSTPLWVLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKEIRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRASKTMKKMEV"
     gene            complement(7333..8091)
                     /locus_tag="LAF92_003605"
                     /pseudo
     CDS             complement(7333..8091)
                     /locus_tag="LAF92_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
     gene            complement(8105..9319)
                     /locus_tag="LAF92_003606"
                     /pseudo
     CDS             complement(8105..9319)
                     /locus_tag="LAF92_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
     gene            complement(9515..9875)
                     /locus_tag="LAF92_003607"
                     /pseudo
     CDS             complement(9515..9875)
                     /locus_tag="LAF92_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9937..10627
                     /locus_tag="LAF92_003608"
                     /pseudo
     CDS             9937..10627
                     /locus_tag="LAF92_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10815..11315)
                     /locus_tag="LAF92_003609"
                     /pseudo
     CDS             complement(10815..11315)
                     /locus_tag="LAF92_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            12987..13532
                     /gene="cobU"
                     /locus_tag="LAF92_003610"
     CDS             12987..13532
                     /gene="cobU"
                     /locus_tag="LAF92_003610"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBI9792550.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            13529..14272
                     /gene="cobS"
                     /locus_tag="LAF92_003611"
     CDS             13529..14272
                     /gene="cobS"
                     /locus_tag="LAF92_003611"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBI9792551.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIIAFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGESILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            14284..15363
                     /gene="cobT"
                     /locus_tag="LAF92_003612"
     CDS             14284..15363
                     /gene="cobT"
                     /locus_tag="LAF92_003612"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBI9792552.1"
                     /translation="MQILADLLNTIPAIDSTAMSRAQRHIDGLLKPVGSLGKLEVLAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AEQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     QELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            15425..16360
                     /gene="ldtA"
                     /locus_tag="LAF92_003613"
     CDS             15425..16360
                     /gene="ldtA"
                     /locus_tag="LAF92_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBI9792553.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPDTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(16415..16490)
                     /locus_tag="LAF92_003614"
     tRNA            complement(16415..16490)
                     /locus_tag="LAF92_003614"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16455..16457),aa:Asn,seq:gtt)
BASE COUNT         4454 a         3779 c         4228 g         4409 t
ORIGIN      
        1 gcacgcacgc gcgataaaga ggggatagtt ttcgtgagga cagggagaga agcaggcgtt
       61 ctgataattg cagagttgtt ttcggaggtg gttacagtga gtcagcccgt tcctggcgag
      121 caggagactg atatcaccga tgggataagg ttgtctgatt ggtattataa atataagaag
      181 tcatcagaga gtaacgtaaa ggatttgcca gaataaccta agagagtaaa tctctccggt
      241 aacagagcgc tgtgaagcat tctgtatcag agtgcagata catgaacgct gatgcgtaaa
      301 atcagcagtg acacgccagc tccccctatg cccattcctg tataagacat gaactgagtg
      361 tattaatgct tctgccctga gactgagccg gtgagtgagg ggctgagaaa tccctcttga
      421 cacagctcta accagaaata cttccccggt tgttgcggtg cgtccggatc cccgataacg
      481 gaacactaag gctgtattgc gggacaccgg gtaagtagca gcccgtaagg cgttctgtca
      541 acgccagaaa aaatgatttc ccacacgcgt tatagactca ctcctgtgga cctggaagcc
      601 cgtaactctg cggttgccca ctgactgacg gaactgaccg caaattttac gagtgcgtgc
      661 tgggcttcag tctggtcacc tgagcgttcg gtaacggagg cgcgggcggt aataggctga
      721 ttttcaggta ttaccggctt acagcgtgtg gttgatattg aggggctgtg agtttcgcgt
      781 atttgtcaga aacccagacg agttttcctg tatgggacga aataaggcag cggcaatacc
      841 tggaaaaatt taatatggta tgaaggagaa cctgtcggtt acgcttaata tacccggtac
      901 actgtcagct caggggatac atgaaccgtt atctcaccag ctacacagta tatacacact
      961 gacgctgaca gtagtcagca ggaccacaat aatcagtgcc agcagtatgg taacgatacg
     1021 gtctgtattc cggtaatagc cgaaggggtc gacagaacca caatacgggc aacacactgt
     1081 attatgagta atcgcgtttc cacagtccct gcacttcttt ttgcgtatca ccatcactcc
     1141 ctcctcacag ccttacccgt aaccggtttt ttacattaaa aaaacaacgc tgaaaattca
     1201 accgtcagtc cggagacgac cgttcgggtt atcacagagt gcctgagaca gtgtcctgcc
     1261 ggaggtcact tcagggactc tgcgcatttt ttacgacgtg gctattccgt atgaaccata
     1321 cggagattta accatgacct acaaatacaa ccccttttgg cagcaacgta ttcgtgagac
     1381 ggtgcggcac gcactgaatg ttcatccccg cctgacggca ttgcgggttg acctgcgttt
     1441 cccggatgta ccggcagcaa cggacgcagc tgtgatatcc cgcttcatca atgccctgaa
     1501 agcccgaatc gacgcttacc agaaacgtaa gcatcgggaa ggtaaacgcg tgcatcccac
     1561 aaccctgcat tacgtctggg cccgggagtt tggggagtgc aaaggtaaaa aacactatca
     1621 cctgatgctg ctggttaacc gggatacctg gtgtcgtgcc ggtgattacc gtgctccggg
     1681 atcactggcc gggatgatta aacaggcgtg gtgcagtgcc ctgggagtgg atgtcgggtg
     1741 ccatgccacg ctggtgcatt ttccggcctg gccggcggtg tggctggaac gcgatgatga
     1801 cactggcttt cagcaggtgc tggaacgtgc tgactatctg gcgaaggaac ataccaaagc
     1861 tcactgcacc ggtgagcgca attttggctg tagccggagt tgagcacgac tggcgcgcca
     1921 atacccggca aaccaaaagt atgggttgtt gtctggcggc aacgatgtat ccactcgccc
     1981 actgactgtt ttttacgtca gtgttccatt gtgtgacaac cacagtgctg taacagcaac
     2041 cagcctgctt tacccaaact ccagtccaga ttttaactga accgccattc acaccctgac
     2101 cacgctgccc gtaccgggac ggctgttgcc tgcgtgtcgt ctggcggctg aggaatatga
     2161 ctatgtacgc aaaatccttt atcgctcttg atggcaacgg acgtctgacg ggcgcccgta
     2221 ctgcacaggc cgcaccttat gctaactaca cctgccactt gtgtggcagt gcactcagat
     2281 accatccgca atacgacact gaacttccct ggtttgaaca cactgacgac aggctgacag
     2341 agcacggtca acagtgccct tatgtcaggc cggagcgcag agaaatacag ttgattaaac
     2401 gtctgcagca attcgtaccg gatgccttac ccgtggtgcg taaagccagc tggcactgca
     2461 gacaatgtca ccacgattat tatggggagc ggtactgcac acactgccag accggaggat
     2521 tcagtattcc ccggacaact caggaggaaa tatgcgaatt ctgaactgtt atatggcgaa
     2581 tgacagcaaa ggccattttg ttacggcgaa agaagcagcg aagcacaacc gccaggacgt
     2641 tttgtgctgt gtgtcctgtg gatgcccgtt aacacttcag cggggcaatg acggacaacc
     2701 accgtggttt gaacatgatc agatgactgt cgctgagaaa atcctgctgc gatgcacctg
     2761 gcttgacccg gcagagaaag aggcccgtcg tttgcatctg cagggtatga cggttccgga
     2821 ttatacggtg aaggtgagaa agtggttttg tgtgatgtgt gacgaaaatt atgaggggga
     2881 aaagtgctgc ccgagttgtg gtaccggggt atacagcagg gagggggggc aggagggcaa
     2941 ctggaaggat aggaactgag cggttcagtc gcggaaatca gttgcagagc tgtaatgata
     3001 tctccgggag tgctgcgttg gtaggggcat cccgggatag agaaatttta gcactttgcc
     3061 ctgaatgatt gtagacgtcc cgagtactcg taccggtctg agttattaca gttagatatt
     3121 cactttttct cccgcatact tcacatctgt caacgacgga tgaaaagtgg ttcttccgca
     3181 acctgggtgg aagaaaatgg atgaaacttt ctggcgaaca aggatctaag ctgttttaag
     3241 ttatgggcaa cgcaatgcac tctcttaaga cacttctaca gttaccttgc ggatggcgat
     3301 gcagtcgaca aattattagc tctgacggta taaccctcca tctccacgga aaacgcaaaa
     3361 cagcacaatg tcctgaatgc tttaagcgta gcgactctgt tcatagttgt cgtcggcgcc
     3421 ggatacagca tctaccctgc tcagggcaga cgctatggct tgtattttcc gtccgccact
     3481 ggtactgccg taacccggct tgttcacgta aaatttttgc cgagtcgctt gctcccttcg
     3541 ccagttcaca ccagcagtct tcacaggcgt tacaaaatta acaacgtcaa ctgggattaa
     3601 tagctggagg tgaggctgga aaacgggctg caacggcagt gggtctccgt tgcagtgctg
     3661 atactcttct tcgcaggctt atcaataccc cggagacgaa acagtcaggc gcgcctcatg
     3721 tcggtattga tgagtgggcg tggcatcggg gccactgctg cggtatgtta atagtcaatc
     3781 ctgatactca ccgtcccctc gtcctgcttc ccggccgtga tcagcgtacg ctggcgacct
     3841 ggttcagaaa atatccggaa atacaggttg tctcgcgtga tcgcagtgga gtctatgcga
     3901 cagcagcacg tgaaggtgca cctcaggcca gacaggtggc cgatcgatgg cacctgctaa
     3961 aaaatattgg tgatgagcct gaacgaatga tgtacagata tatgcctctg atacgtcttg
     4021 ttgtccgaga gttatcactg aagaaatcac ctgagccaga aatatctgtg cctgtagcat
     4081 cgctccgtcg tctggaacgc cttaaacagc acatccgcaa aaaacggcat cagcgttgga
     4141 cagaggttat ggccctgcat aacaagggat gtagtttcag ggaaatatcc cgtattacag
     4201 gcctgtcgcg agtgacagtc agtcgctggg tgggttcagg aacattccct gaaatgtcaa
     4261 ctaggcctcc aaagcgaggg cttctggacc catggaggga gtggttaaaa gagcaacgag
     4321 aatgtggtaa ttataactcc ggccggatat ggcgggaaat ggtggccagg ggggttacag
     4381 gcagtgaaac catcgtcagg gatgctgttg ccaaatggca taaaggctgg atcccaccgg
     4441 ttactactgc cgcaagactt ccttcagtgt cccgggtaag ccgctggttg atgccctgga
     4501 gaataatcag gggtgaagaa aattatgctt tccgatttat tagtctgatg tgtgaaaaag
     4561 aaccggagtt gaaaatagcg cagcaactgg tactcgagtt ctaccgtatt ctgaaaacct
     4621 aaaataaatc acagctaagc agctggttca ctcgggtcca cgaaagcggc tcagcagaac
     4681 ttcggtgcgt agctgcgggg atggaagctg atgctgcggc tatatgtgag gcaatcagca
     4741 gtcgctggag tactggtgtt gtcgaaggtc atgtaaatcg cctgaaggtg ctgatacgcc
     4801 agatgtatgg tcgagccgga cttgaactac ttaggcggag ggtaatgagt ccactggcat
     4861 gaaaatcttc acctaggttg aggaagagcc acttttgcac tgttcgtgac agtatggcct
     4921 ttatgtactc actttgttac cggtagctgg atcatcacta ccgtgccgca aatggtggtg
     4981 aacagcatca attgtggaat gaatgttgcc gaagcgacca atgcgcctcg tttccaccat
     5041 cagtggttgc cggacgagct gcgtgttgaa aagggtttag tccggatacg ctcaagctgc
     5101 tggaagcaaa aggtcagaaa gtggcgctga atgaggcgat gggcagcacg caaagcatta
     5161 tggttgggtc ggatggtgag ttgtacggcg catccgactc gcgcttggtg gatgatttaa
     5221 cggcggggta ttaattgaca tcccccggcg caatgtgccg ggggataatg gttttatgcg
     5281 gtcaataatc gtccgggatt atcgatacag agttttttga ggatcgtttt atcgacgccg
     5341 cgctccgcca gataggccag aaaaacatct ggaacaaatc cccagccatt accgccattt
     5401 ttagcccaca tttgtttaag gaagacatcg tggctgagaa caagctgatc ggcgtatccc
     5461 aactcaatga gatgagcgac ggcatcggca gtctcctgca cccctggcgc tattccctct
     5521 ttcgggaagg taatgtctag gccaatcatg tcgaattcca gccagacacc tttatcaagc
     5581 attttccgct gatacgccac gtcttttcct gacggatccg agtgtgcgag agagacttta
     5641 tttggcgaga cgcccatttc acctaacaca atgtcgagta cttcatcacc gcgacgaagc
     5701 cagcccggca tgtgaatatt catcgccaca tgaggattgt taatctgtgc cagcgaggca
     5761 gcccgcaagc tgttatgctc ggcttcggta aatgtcggtg agacacctat ttcaccgatc
     5821 attccggcac gaatatccgt atcgccaatc ccttgattca attctttatc gatcgttgtc
     5881 gctagttcat caaccgtttt atgaattctc tggctttcaa atttttccag gtagggaccg
     5941 gaggaagcaa caatattcag acctgttttt aatgcgactt ctcgtaatgc ctgcgcatct
     6001 cgtccgatag actcagaccc tgtggcatca acaatggtgc gaccaccgag cgagataaag
     6061 ttattaattt cgaagatcac atcttcaata ggtttgcggt ccatattatc agcgcagcaa
     6121 taaggatcgt gttttaatgc ccactggatt tctgccgtga cttttttatc aacaagctgt
     6181 tgtgaaaaag gatagcaagg agcatccaca acagaggaaa gatcgttgaa cagatgttca
     6241 tgaggaagtg ttagccccat atcttcacgg gcgacggggc ccgtcacagt ttgtagataa
     6301 tctttcatga ttatacctcc attttcttca ttgttttcga ggctctgtat tccggaatag
     6361 tggtaacaat tgcaccgacc agtgcaaaca gtgttccgat gatcgtgacc agataaacgg
     6421 tattgcccag cgatggaatt aatacgtcaa tcaatactga tcccagtaac tggcccgccg
     6481 ttgaagcaac ccccagcatc agtaacccca gacccctgac caaaatggcc attaaaccga
     6541 tagacattag tccaagcggg ccgccaagat acatccacca gacatctggt aattgaacag
     6601 tgacgtgccc taatgcgaca cgaatagcta gcgctgtgcc taaaacacaa aatccaacga
     6661 tgaaattcca ggtgattgaa accagcatgg atcccgtagc ttcagctact tttgcatttc
     6721 cagcgggctg ccatcctgca agcaaaccag ctaagaacgg aagaatggca agtaaaatga
     6781 aagaggtaga atgccactgc ggtgacacaa caaaaatcgt agcaataacc gcaaataatg
     6841 caccgatgat gcgataaggg gtaaagtatt ttttctcgtc gacaccgata ccaaagcgat
     6901 cgcaaagtaa accagacagc aatagcgcgg agatcagtgc ggtctgaaac gttgcgaccc
     6961 cgagtgcgct ggctgatgcg ccttcagaaa ataccaccat cgctccacag agaccggcaa
     7021 accagttcca gagaggaact tttcggtttt taatcagaga aggaattgcc gcaaattgtt
     7081 gccggatttc ttttcgcgcc ataataataa aaaacatcac gactaaacca ctggcaaagg
     7141 agataactgc gcatgcatta ccgtcctgta accagtgacc taattgacca ttcaccgctg
     7201 attgcatagg ggagagcata cccgcaagaa tcgtggcaag cacccataag ggagttgagt
     7261 acttattctt tttcatacat caatccttct attatagtaa atggagtaac tggtgttacc
     7321 ccattattat tattatcttg caatatgagt cagtatattt acccacaacg cggtatagaa
     7381 cggccaggac ataaactgct gtgtacccca gtgcggtgaa cagtcactca taaagcaggc
     7441 tattttcccg ttgtgatagt taccaaagac cagtaatgga tcgttgttta ttgttaatac
     7501 cacctcagca ttttctttag cgatcgcccg gttataacct aggaaaaaag gatattcaga
     7561 gaacccttta ataaccggat ggtcaggttg tgatggctgt gcaataacac cctctggttt
     7621 ttcgacacga tcgtcaccat ctaacatcgt aaccgggagc acatcagcca gaactgtatt
     7681 tttataattg gcttttgctt caattcccat gaatgagaga tagccgccaa tcatcaataa
     7741 cccaccaccg ttattcacat attatttaat cagttcgaga gcgtttggtt ttatgcgaag
     7801 ctgataaaaa gtatcatttt gtagcaggaa agtattacta ccaatatcac taataacgat
     7861 ggcatcatat tgttctaact gtgccacatc ttcagggaag gcaatttgta ccgtatgtgc
     7921 cggcatatag gtgacatcta cctgactatt tttcagacat tggagtaacc aggtcgcacc
     7981 ttcttcatat ttacttgagg taaaactgtc atatcctttt gaatgaatca tatgaatatg
     8041 ccaggattct ccaataaaga gcacctttag tgattttttg gtgttattca ttctctgtcc
     8101 cttcttaatg cgtaaaaata gtttgctgat aatgtatact ctgaattcga tgtatcacag
     8161 attcatgttc aggcatgtcg gaagcattgc ttgtctccac cgccagggat gcaaaggcgc
     8221 tggcataaca cagtgctgat tccagtgatt ttcctttcgc aagtcctgat gccagagcgc
     8281 cgttaaaagc atccccggct cctgccgtat ttttaacgac cgctggaaac gctggcgaat
     8341 gaatgaattt tttgccatca aatgcgagcg agcccttgct accgagcgtg ataacggtgt
     8401 tttttacccc tttattgtgg atagcttctg cagctcgttt agctgattca agatcgtgta
     8461 cgtctatgcc ggacagtaaa ccagcttctg tttcattggg ggtgatataa tcaacatcct
     8521 gaataagttc gttgacgata tcattatagg gtgccgggtt aataatgacc gggatactgt
     8581 ttttttgcgc caatgtgatc gcttgttgca gagcggtata atttgtttcg agctgcagaa
     8641 gtatgacatc agaattaata atcgcttctt tctgaatagt tatttcatcg gacgacattg
     8701 tcatgttggc accgggatag atcgcaataa cattatcgcc agtaccttca ttaacaagga
     8761 tcgtggcagt tccggtttgc gtttctttcg tttgataaat tatactttta taaatcttag
     8821 acgaattcat aaagttgatc gcataatcac taaagtgatc tgtacctact tttgttatga
     8881 aataaacatc agtatcggca aagcttgctg ccagcgcctg gttacaacct tttccccccg
     8941 gtgaaaatat aaaattactt gccagaagcg attctccgat agtgggaagc ctcggcaaat
     9001 aactgatgat atcaacatta aacgaaccaa taacgcatac ttgacttttc atagttgagt
     9061 ggcctttttt tattccctag gctgtatcat aattttcaag cgcataagcg atttcatttt
     9121 tcgcaatagt atcaagcgag tctaggacga taaaagcacc gccatgatat cgagtcagat
     9181 agccaagtct ggcaagttca ttaagatctg atcgaatagt ttctttcgtt acattgaggc
     9241 tattggacag atcggatact tttacagaac cgaatttact gagtcgtttc aatatttcat
     9301 gatggcgttc aaatttcatg cttcacctca cttcagttac gttaatatct gtcgatgtat
     9361 attgatgtga taatcaccat aaagtggcgg ggaaaagcaa aaaaaatcaa aaatagccgg
     9421 agaaaaggta agcgttaatg gagaatcata ttgatgctta tttattggtg agaactacgt
     9481 tccatggcag gagttcatca atacggttgg aaggttattc cggcagtacg ctcaggatat
     9541 ggcgcagata ctcttccgga tcaatgtcgt tcagaaggca agtgccgata agcccataca
     9601 acagtgcact gcgctcgccg tcgaagaaca taaagttttt atttccgaga cagatggcac
     9661 aaagggcttt ttccgcggca ttatgtctgc ctccgcctga ccgtcaacac tgtaataaca
     9721 gagtgcgccc cactgattca gtacacagct gaaccattct cccagtctgg atttttggac
     9781 agcgtggcat ttttctccac catccacgca tgcagtgagt ccagtaacag tttgctcttc
     9841 ctctgcctga ccacaagatg ttctggttct ggcatactga atttggccac ctgagcatag
     9901 gtaatatgct caccttagaa caacacagat gctccaatga aaaaaggaaa tttcagcgca
     9961 gagtttaaac gcgaatccac tcaactagtt gttaaccaga actttacggt ggcaggtacc
    10021 atccaaagct atggatgtcg gtctttccac aatgacaaga taagtcaaac aactgcgtaa
    10081 tgagcatcag agcaaaacac caaaagcctc tctgatgaca ccagaacaaa tcgaaatacg
    10141 tgagctgagg aaaaagcggc aacgtattga aatagagaat gaaatattag aaaaggctac
    10201 ggcgctcttg atgtcagact ccctaaacag ttctcgataa tcgagaagct cagagcgcat
    10261 tatctggtga tagcactctg ccatgtgtgc aggattcatc gaagcagcta cagagattgg
    10321 aaaaaccgtc ctgaaaaaaa gacggtagac gggctgtatt acgtagtcag gtaattgagc
    10381 ttaatggcgg agcaaggaac atcgccacaa ttgcaacccg gagagactac cagatgggac
    10441 gcaagcttgc tggcagtctc atgaaagaac aggggctggt cagctgtcag ccgttgaccc
    10501 tccggtacag gcatggcagt catgaacttg tagctatcct aagaacttga agggcgctta
    10561 gctgtgacac agctaaatca agtatggtga cgtgacctat atctggactg gtaaacgatg
    10621 gggatacccg cttacgcgag agcaataagt ggctggccac cattctcgat acaaaactct
    10681 gctgaatagc agcgattttc ccagcgtggt gcgtcttttg cattttactg gttctgagtt
    10741 atcaattatc tgttcactaa tgactactct aaaaagttgt atcaatttaa gtgatatagg
    10801 gagtccggag attcaccctt tttggtgatg aagttaacaa taccgccaat tgcctgtgaa
    10861 ccgtacagta cggaatatgg acctttcact acctcaacac gctccagcgc agactcatct
    10921 atcaacagtc ccacaccata attatctccg gcgcgctgat aagttacctc ctgaccatca
    10981 atgagaatta aaacacggga ggatgcttcg ccacgaatgc ggatttgttt acggcctgcc
    11041 aaggagttgt ctgttatctc tactccgggg atgtcctgca aattatcggc tatggaggca
    11101 ttggtggagt tctgcaatgt ttgttggtca ataacctgta tagtcgccgg gctttcccaa
    11161 aggttgcttg ccgaacgatt cgtggtcacg acgagcgtct cttcgttgct gatttttgtt
    11221 tctgaagaag ttgcggttgc agtagaaact atcccagaaa tgcataatga cagagtggaa
    11281 aggatataaa ttcgacaagg tatattatat aacatttcat ccctcattaa tacaaagtac
    11341 aaatgattgc tgtaatgata attattttca tttatgttct catatatcta taattattgt
    11401 catcttcata aattgtaaat atcaaacatt tttgacctta atcatgtttt ttgatagata
    11461 ttttacgact gaacatatcc gtcgtttcct ctgactcgtg tcagtcggtc tctggttgta
    11521 tccacggacc gcactcttga cataagagtg cggtccgtgg aaaacggagt tgaaaaaatg
    11581 gtgtctgaaa gaaggtacca tcattgtgat gacaaatact ttggcgtgga taatgtaatt
    11641 aattgctgaa attgaccgta tatgcggtaa gactcatatc agtatcaggt ccggttaatt
    11701 gctgatacta atataaactt tttacaaaag gatgaatgca gaaggttact ctaatggcca
    11761 gagtgatgac aatgatggtc agacgttgac ctgcagaaac tgcattttaa ttggtctata
    11821 aagtctgcaa gaggaatcag ggtcagatca gtgagtctta tcgcggcccg aagctatgac
    11881 tgaaagaagg ctggatacag aattgcaaca atttggcaat gtcagaacac agaagctttg
    11941 caagcaggtg aaaggttatg tctagcaaaa ggagtatatc agaatatatc tactgatgta
    12001 caaaacacaa caatagcttc atgatatata aatacgtgat gacgagtggt actccgcatg
    12061 gttatttaac ttctcagcac cactaatgat cctgagcaac ttcgtatttg ggccattacc
    12121 atggagtaga aagctgtgtc tgagaatgca gaatttgaga aaagaattcg ctttcttgga
    12181 gagcttctga aaatggcacg ctaacaacgt tttgttaaaa aatgtgaatc acttgccggg
    12241 gctgcatttt ctattcgagg agaatgttga cgtagatatt acagaaatct cagcgcatca
    12301 ggataaactg ctaccttagg tcaaccacca aaagaaaata caatacgtcc agtttgtatg
    12361 cctttaataa cacatcttct aaatgttgaa gaattatccc gcctgctgaa gaacgttgct
    12421 ctgagtgtga taaacctttt tattcttgat gatgtcagcg agaagatgga atatatcccc
    12481 gactgggtcg tggttacccg gttaaacatc tggtggcgct aataaatctg gcaagtcaca
    12541 ttctgttgaa tgctgtgaca gtgccttacc gagagagcga aattgtggtg ttctagtgca
    12601 tagtcgccac cattccggat aattctatta cgttgtttta caagttcttt tacagcgcag
    12661 atctgtcgct gacgttgaat caatagcctg gatttcagaa cacaatgccc gtttgctggt
    12721 tgttccggca gtagcattaa ttcattatct gaatgaaatg tgtcaatcca cagaaaccgc
    12781 tgtcaatgac gcattttgtc ggacgttaac acgagaaagt ctggctattc cgtaatgcca
    12841 tcttatttcg taatggattg agaatgctgg atttttaatt atattacgcg cagtattaat
    12901 cgcgtaaatg ttttgcggtc agcaacggtg ggggatccat tgttctgttc aatgaatacc
    12961 atcatacatt tgaatcagga gtcggtatga tgattttggt gacggggggc gcacggagcg
    13021 ggaagagtcg ccacgcagag gcgcttattg gggactcttc acaggttctg tatatcgcta
    13081 cctcgcaaat ccttgatgat gagatggctg cacggataga acatcatcgg caaggccgcc
    13141 cggagcactg gcgcacagtg gagcgctggc aacatcttga tgaattaatt catgcagaca
    13201 ttaacccgaa tgaggttgtg ttgcttgaat gcgttaccac aatggtgact aatctgttgt
    13261 ttgattatgg cggcgataaa gaccctgatg aatgggatta tcaggcgatg gaacaggcga
    13321 ttaatgctga gattcagtcg ttgattgctg cctgccaacg ttgccccgca aaggttgtat
    13381 tagtgactaa cgaagtggga atggggattg tgccggagag tcgtctggca cgacattttc
    13441 gtgatattgc cgggcgggta aatcagcagt tggccgctgc ggcaaatgaa gtatggctgg
    13501 tggtttcggg tattggagta aaaatcaaat gagtaaatta ttttgggcga tgctctcatt
    13561 tattacgcgt ctgcctgtac cgcgtcgctg gtcccaggga ctggatttcg agcattattc
    13621 tcgcggtatt attgcttttc ctttgattgg attattgctt ggcgcgatta gcgggctggt
    13681 cttcatggtg ctgcaggcat ggtgtggcgc accactggcg gcactgttta gcgtacttgt
    13741 gctggtgctg atgaccgggg gattccatct ggacggcctc gctgatacct gtgatggcgt
    13801 attttctgca cgtagccgcg atcgcatgtt ggaaatcatg cgtgatagcc gtttaggcac
    13861 ccacggcggt ctggcattga tttttgtggt actggcaaag attctggtgt taagcgagtt
    13921 ggctctgcgt ggcgaatcga tccttgcatc gctggcggca gcatgtgcgg tcagccgcgg
    13981 gactgccgca ttattgatgt atcgtcatcg ttatgcgcgg gaagaagggc ttggcaatgt
    14041 gttcattggc aaaattgatg ggcgacaaac ctgcgtcaca ctcggcttag ccgctatttt
    14101 tgctgccgta ttattgccgg gtatgcatgg tgtggctgct atggtggtga cgatggtggc
    14161 gattttcatc ctcggtcagc ttcttaaacg tacgctgggc gggcaaacgg gcgatacgct
    14221 gggagcagcc atagagcttg gtgaactggt cttcctgctg gcactgctat aaagagacgt
    14281 gagatgcaaa tacttgccga tttactgaat acgatcccag ccatcgattc taccgctatg
    14341 tcgcgtgcac aacggcatat tgacgggtta ctcaaacctg ttggtagcct gggaaagctg
    14401 gaggtgcttg ccatacaact ggcaggaatg ccggggttga atggcatacc gcatgtgggc
    14461 aaaaaagcgg tactggttat gtgtgccgat cacggcgtct gggaggaagg ggtcgctatt
    14521 tctccaaaag aagtgacagc catacaggct gaaaatatga cccgtggaac aaccggcgtg
    14581 tgtgtgctgg cagaacaagc gggcgctaac gtccacgtaa ttgatgttgg tattgatact
    14641 gctgagccta tccccgggct tatcaacatg cgtgtcgcac gaggtagcgg caatattgct
    14701 tcagctccgg caatgagtcg ccgtcaggct gaaaagttgc ttttggacgt catatgttat
    14761 acgcaggagc tggcaaaaaa cggtgtcacg ctgtttggtg taggtgaact ggggatggca
    14821 aacacgacac cggcagcagc aatagtcagc acaatcactg gccgggatcc tgaagaagtg
    14881 gttgggattg gcgcaaacct gccgacagat aaactggcta ataaaattga tgttgtgcgt
    14941 cgggcgatta cgttgaatca accaaatcct caggatggtg ttgatgtcct ggcaaaagtg
    15001 ggtggatttg atttggtcgg aatagctgga gtgatgttag gtgctgcttc ctgcggttta
    15061 cccgtgttgc tggatggatt tctttcttat gctgctgcgc tcgcagcctg ccagatgtct
    15121 cctgcgatca aaccgtatct cattccttct cacctgtcgg cagaaaaagg cgcgcgtata
    15181 gcgctctcgc atttggggct ggagccttat ctcaatatgg agatgcgttt aggtgagggg
    15241 agtggcgcag ctctggcgat gcccatcatc gaagctgcct gtgcgatata caacaacatg
    15301 ggcgaacttg ctgccagtaa tattgttcta ccggggaata cgacttctga tttgaacagt
    15361 taaaaacgaa aagtctgata aaagctatac ttaacggata gctttcgcga cataggaaag
    15421 ggacatgatg cgtcgtgtaa atattctttg ctcatttgct ctgctttttg ccagccatac
    15481 tagcctggcg gtaacttatc cattacctcc agagggtagc cgtttagtgg ggcagtcgtt
    15541 tactgtaact gttcctgatc acaataccca gccgctggag acttttgccg cacaatacgg
    15601 gcaagggtta agtaacatgc tggaagcgaa cccgggcgct gatgtttttt tgccgaagtc
    15661 tggctcgcaa ctcaccattc cgcagcaact gattttgccc gacactgttc gtaaagggat
    15721 tgttgttaac gtcgctgaga tgcgtcttta ttactaccca ccagacagta atactgtgga
    15781 agtctttcct attggtatcg gccaggctgg gcgagaaacc ccgcgtaact gggtgactac
    15841 cgttgaacgt aaacaagaag ctccaacctg gacgccaacg ccgaacactc ggcgcgaata
    15901 tgcgaaacga ggggagagtt tgcccgcatt tgttcctgcg ggccccgata atcccatggg
    15961 gctgtacgcg atttatattg gcaggttgta tgccatccat ggtaccaatg ccaattttgg
    16021 tattgggctc cgggtaagtc agggctgtat tcgtctgcgc aatgacgata tcaaatatct
    16081 gtttgataat gttcctgttg ggacgcgtgt gcaaattatc gaccagccag taaaatacac
    16141 cactgaacca gatggctcga actggctgga agttcatgag ccgttgtcgc gcaatcgtgc
    16201 agaatatgag tctgaccgaa aagtgccatt gccggtaacc ccatctttgc gggcgtttat
    16261 caacgggcaa gaagttgatg taaatcgcgc aaatgctgcg ttgcaacgtc gatcgggaat
    16321 gcctgtgcaa attagttctg gttcaagaca gatgttttaa gagcgttggt aattagaaag
    16381 caaaaagcct gctagaaagc aggctttttg aatttggctc ctctgactgg actcgaacca
    16441 gtgacatacg gattaacagt ccgccgttct accgactgaa ctacagagga atcgtgtgaa
    16501 cggggcgcat attaatggca gtgcatggtg atgtcaaagg gtgaaatgca tttttttgtt
    16561 tgtttgctgt caatttcaac aaagtggtga gctttcaatc ttattgggta atgaaccatc
    16621 gtggtgcata ccctcctttt atagggcagg ggaacgcgac agctgattaa aggagcaaat
    16681 gacgaatcta tcataatctc ttatttgaca gttggttagc ttgtacatca acaccaaaat
    16741 aaaactggca agcatcttgc aatctggttg taagtaatgg cggcacttgg gccgattctt
    16801 aaaaaccgga ggcaacatga acttcagacg cctgaaatac ttcgtaaaaa ttgtagatat
    16861 tggtagcctg
//
LOCUS       DAEVZA010000107        16869 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEVZA010000107 DAEVZA010000000
VERSION     DAEVZA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.107"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1133)
                     /gene="puuB"
                     /locus_tag="LAF92_003615"
     CDS             complement(<1..1133)
                     /gene="puuB"
                     /locus_tag="LAF92_003615"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBI9792554.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGYGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKMLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRL"
     gene            complement(1135..2622)
                     /gene="puuC"
                     /locus_tag="LAF92_003616"
     CDS             complement(1135..2622)
                     /gene="puuC"
                     /locus_tag="LAF92_003616"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBI9792555.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYSAAAENETFETVDPVT
                     QAPLANIARGKSADIDRAVSAARSVFERGDWSQAAPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHDDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHGFIREGEGKGQLLLDGRNAKLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(2787..3344)
                     /gene="puuR"
                     /locus_tag="LAF92_003617"
     CDS             complement(2787..3344)
                     /gene="puuR"
                     /locus_tag="LAF92_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBI9792556.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSIKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(3371..4135)
                     /gene="puuD"
                     /locus_tag="LAF92_003618"
     CDS             complement(3371..4135)
                     /gene="puuD"
                     /locus_tag="LAF92_003618"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBI9792557.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            4347..5765
                     /gene="puuA"
                     /locus_tag="LAF92_003619"
     CDS             4347..5765
                     /gene="puuA"
                     /locus_tag="LAF92_003619"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBI9792558.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            5914..6078
                     /locus_tag="LAF92_003620"
     CDS             5914..6078
                     /locus_tag="LAF92_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792559.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            6068..7453
                     /gene="puuP"
                     /locus_tag="LAF92_003621"
     CDS             6068..7453
                     /gene="puuP"
                     /locus_tag="LAF92_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBI9792560.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            7587..7832
                     /locus_tag="LAF92_003622"
     CDS             7587..7832
                     /locus_tag="LAF92_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBI9792561.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            8145..9788
                     /gene="sapA"
                     /locus_tag="LAF92_003623"
     CDS             8145..9788
                     /gene="sapA"
                     /locus_tag="LAF92_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBI9792562.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            9785..10750
                     /gene="sapB"
                     /locus_tag="LAF92_003624"
     CDS             9785..10750
                     /gene="sapB"
                     /locus_tag="LAF92_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBI9792563.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            10737..11627
                     /gene="sapC"
                     /locus_tag="LAF92_003625"
     CDS             10737..11627
                     /gene="sapC"
                     /locus_tag="LAF92_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBI9792564.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            11627..12619
                     /gene="sapD"
                     /locus_tag="LAF92_003626"
     CDS             11627..12619
                     /gene="sapD"
                     /locus_tag="LAF92_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBI9792565.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            12621..13427
                     /gene="sapF"
                     /locus_tag="LAF92_003627"
     CDS             12621..13427
                     /gene="sapF"
                     /locus_tag="LAF92_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBI9792566.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            13495..13848
                     /locus_tag="LAF92_003628"
     CDS             13495..13848
                     /locus_tag="LAF92_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBI9792567.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            14218..15006
                     /gene="fabI"
                     /locus_tag="LAF92_003629"
     CDS             14218..15006
                     /gene="fabI"
                     /locus_tag="LAF92_003629"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBI9792568.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            15150..16277
                     /locus_tag="LAF92_003630"
     CDS             15150..16277
                     /locus_tag="LAF92_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBI9792569.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLRHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMDSSNEYKKISTLMRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            16345..>16869
                     /locus_tag="LAF92_003631"
     CDS             16345..>16869
                     /locus_tag="LAF92_003631"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Involved in mRNA degradation. Hydrolyzes
                     single-stranded polyribonucleotides processively in the 3'
                     to 5' direction; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBI9792570.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAP"
BASE COUNT         3741 a         4461 c         4480 g         4187 t
ORIGIN      
        1 atcaaacgtc cggctagatg agtacaggtc acgccgtggc cgctgtagcc ctgcatgtaa
       61 tagatgttgg tatcgaggcg accaaactgc ggcatacgcg acagggtcag caggaagttg
      121 cccgtccagc ggtaatcaat tttcacgccc ttcagctgcg ggaaggtttt caacattttc
      181 ggcaccacaa ggcgctcaac gtcatccggg tcgcgcgcgc cgtagaccac gccgccgccg
      241 tacagcagac ggttgtcggc ggtaaggcgg tagtaatcaa gcagatagtt acaatcttcc
      301 acacagtagt ttttcggaat caacgaacgg gctaaatcat ccgacagccg ctcggtggtg
      361 atcacctgcg tgccgcacgg catgctgcgt ttcgccagct ctggctccac tttatcgccc
      421 aggtacgcat tcccggcgac aatcacatac ttcgccgtca cctggcctgt ggctgtacgc
      481 actacggcgg gcgtggtgtg ctggatctgc gtcacggcag aaagctcata cacgcgcccg
      541 ccgttcaggc ggatggcgtc ggcttcacca atggcgaggt tcaacgggtg aatatgcccg
      601 ccgctgtgat caagcagcgc gccggtatag cgatcgctgg cgacctcgcg gcgaatggcg
      661 ttggcgtcca gcagttccag ctgtttattg ccgtagcgtt cccagttctc tttctgctct
      721 tcaagtgtgg cgagttgttt atcattcatc gccacaaaca ggccgccggg gcggtagtcg
      781 cagtcaatct gataacgttt gatgcgctcg cggatgatct cgccgccttc aaacatcatg
      841 ctgccgagca tacgggcggt gtccatgccg tagctttttt cgataacgtc gatgtcgcgg
      901 ctgtaggagt tcacaagctg cccgccgttg cgcccgctgg cgccatagcc gatgcgtgag
      961 gcttcgagaa ccactacgtc aaaacccgct tccgccagat gcagcgcgga agagagcccg
     1021 gtatagccgc cgccgaccac gcaaacatca caggtgattg actcattcag cgtgtcgaat
     1081 ggcgcgtatt tattcgcact ggcggcgtaa taactgctgg tatgttcggt catttcaggc
     1141 ctccaggctt atccagatgg ttttcagttc ggtgaatttt tcaagggcat gcagggattt
     1201 gtcgcgaccg ttgccgctct gcttatagcc gccaaacggc acggtcatat cgccatcgtt
     1261 atagttattg acgaagacgg aaccggcttt caggcgacgg ctcatacggt gggcgcggga
     1321 gagatcgcgc gtccacaccg ccgcgccgag gccatactgg ctgtcattgg caagctgtag
     1381 cgcctgatct tctgatgtga aacgggtgac caccagcacc gggccgaaaa tctcttcgcg
     1441 gcttaaggac gcattggggt ccacatccac aaagatggtc gggccgatgg ccgcagccag
     1501 ctttgcgtta cgaccatcca gcagtagttg ccctttgcct tcgccttcac gaataaagcc
     1561 atgcaccgag tcagcgtgag cgctgtcgat taaggtgccc atggtagttg cggggtcaag
     1621 cggatggccc ggctgccagt tttgcgcctg ctgttttaac agggctaaga attcatcggc
     1681 gatgctctct tccagcaaca ggcgcgttcc ggcgatacac acctgcccct ggttgtagaa
     1741 aatgcccgca gcggtcgcac tcgccgcttt ttgcagatcc ggacaatcgg cgaaaacgat
     1801 gttggcgctt ttgccgcccg cttccagcca gacgcgtttc atgttgctgt cgcccgcatc
     1861 tttcagcagc tgtttcccgg tacgggtcga gccggtgaag gcgatggcgt cgatatcgtc
     1921 atggcgtgac agcgcctgcc ccgcttcatg accaaaaccc gtcaccacgt tcaacacacc
     1981 atccggcaag cctgcttctt tcgccagccc ggcgagacgt atcgcactga gcggtgattt
     2041 ttcagacggt ttcagaacaa cgctgttccc tgctgccagc gccgggccaa gcttccagca
     2101 ggtcagcagc agcgggaagt tccacggtac tatggcggca atcacgccga ccggttcacg
     2161 cacgatcatc gccagctcat ggctactggt ggtcgccact tcgccataca ctttgtcgat
     2221 tgcttcggcg taccagcgaa tggcgcgcgc cgcgccggga atatcatcac gcaggctgtg
     2281 acgaatcggt ttgccggtgt cgagggtttc cagtagcgcc agttcttcgg cgtgggcttc
     2341 cattaaatcg gcgagtttat tcagcaccgc tttacgtttt gccggggcag cctgcgacca
     2401 gtcgccgcgt tcaaatacgc tgcgtgctgc gctcaccgca cggtcgatat cggcgctctt
     2461 gccgcgcgcg atattcgcca gcggtgcctg ggtgaccgga tcaacggttt caaaggtttc
     2521 attttccgcc gcagcggaat attcaccgtt aataaataag cgggtttcaa tggcgagact
     2581 taacgcttta tcctgccagt aagccagatg atgaaaattc attatgactc ctgtttcacg
     2641 tctatcagat atatgcagtt tgtgaacgtc agggcgcaga gatccccggg cgatgagccc
     2701 gtttatttcc ggcgacattc tttatagatt gcctttatag ctagccgggt ggcgtttcgc
     2761 ttacccggcc tacaaaagca aaaagattaa aacgtggtgg gcgtatgggc gctgataatt
     2821 cggcaaatgc ctgccgacgt attactgaaa ctgtgcggaa tgccggtatt aatggcataa
     2881 ctttgccccg cgacgagatg ataatcctgg ccattaatgg tcagaacaat ttcaccttcc
     2941 agtacagtgc ctatttcctc accctgatgc ttaattcttt ccccagtggt tgtgcccggc
     3001 tggtacgttt caaagatcat cgccagcgtg cgattcgggt taccgttatg aaccagcttt
     3061 attgacacac cctgactgcc catctcaatt aagtcgtcct gattaatgac gacctgcggc
     3121 tcatcaggtt tttccggctc ggaaaagaat tccgagagtg acagaccata caccttcagc
     3181 agcttttgca gcgtactgat ggcagggctg actttatctt gttctatcgt actgatagca
     3241 ctgtgagtca gcccggagag ttcggcggca cgacgttgtg aaagcccctg ctgctggcgg
     3301 atttccgaca agcgttttcc tggcgccagt ccctcatcac tcatatttgc atttccttaa
     3361 actgtagtgg tcagagtcgc tgtttttcag cgatatggtg ctgacaagcg gtgataaagc
     3421 cctcgaacaa tatacgcgaa agcgcgtact cgctactgtt ccattccggg tgccactgta
     3481 cgcccagcgc aaaaggatga ttgatgacgc taaccgcctc aactaagcca tccggcgagc
     3541 gagcttcaac acgcaaccgt gggctaacga ccttcgcccc ttgtccatgt agagagttta
     3601 cccaaaagtt gctacattca ggtaacaacg cagacagtaa tcccccctct tcaacctgaa
     3661 cttcatgcga cggtgcatat tgctgttcca ccggcagttc gggatcttcc cgatgttcca
     3721 gcaattcagg ctgttcgcac agcttgcgat gcaacgaacc accggttgcc accaccagtt
     3781 cttgtaaacc ccggcagatg gcgaaaatgg ggatgcgcct ttcgagtgcg gcattaatta
     3841 acgccatgct cagaagatca cgcccgggat cggcgtcagg ctcatcgccg ttttcaccat
     3901 ataggtgcgg ctgcacattg ctgggactac caggaagata aatgccatcg agtttcggca
     3961 aaagttgttc aagtaatgac ggttccgcca gcgcatgtgg tagcgcaatt ggcaagccgc
     4021 ctgcgtggat gatggcattc aggtactttt cttgcagagt ctgggtcgca tgacccttaa
     4081 gcctgttcct gcacattacg acaccgataa ccggattgtt cattatattt tccatgctca
     4141 atctcacaaa gtggactaaa ttatcgccat tactgcggtt taaatgacct ttatgttcaa
     4201 tattttttca atctagcagt gggttttcat ttttgcaaac tcaatttaac atttgacaaa
     4261 catttagttt gcatacagat tcgaatggtg gtcattatat tttacgcttt gataacgagc
     4321 ggaaaacaaa ccaaaggcga agaatcatgg aaaccaatat cgttgaagta gagaactttg
     4381 ttcagcagtc agaagagagg cggggtagcg cctttacgca ggaagtgaaa cgctacctgg
     4441 agcgctaccc gaatacgcaa tatgttgatg ttttacttac tgatttgaat ggttgcttcc
     4501 gtggaaaacg tatccctgtt tcaagcctga agaagttgga gaaagggtgt tatttcccgg
     4561 cctcggtgtt tgcaatggat attctgggca acgtagtaga agaagcgggt ctgggtcagg
     4621 aaatgggcga gccggatcgt acttgtgttc ctgttctcgg ttccttaacc ccttctgccg
     4681 ccgatccaga gtttatcggt cagatgctcc tgaccatggt cgatgaagat ggcgctccct
     4741 ttgacgttga gccgcggaac gttcttaacc gcctgtggca gcagctacgc cagcgcggat
     4801 tgttcccggt tgtagcggta gagctggagt tctatttact ggatcgtcag cgcgacgctg
     4861 aagggtatct gcaaccgccc tgcgcgcccg gcaccgatga ccgcaataca caaagccagg
     4921 tttactccgt tgataacctc aaccacttcg ccgatgtgct caatgatatt gatgaactgg
     4981 cgcagttaca gttaattccc gccgacggtg cggtcgccga agcctcgccg ggtcagtttg
     5041 aaatcaacct ttaccatacc gataacgtgc tggaagcctg cgatgatgcg ctggcgctaa
     5101 aacgcctggt gcgtctgatg gcagaaaagc ataagatgca cgccactttt atggcgaagc
     5161 cgtatgaaga gcacgcgggc agcggaatgc atatccatat cagtatgcaa aataaccgtg
     5221 gcgagaacgt gctttctgac gcggaaggcg aagattcgcc gctgctgaaa aagatgctcg
     5281 ccgggatgat tgacctgatg ccgtcgtcga tggcgttgct ggcaccaaac gtgaactcgt
     5341 atcgccgctt ccagccggga atgtatgtgc cgacgcaggc gtcgtggggc cataacaacc
     5401 gcaccgtcgc cctgcgtatt ccgtgcggcg accgtcataa tcaccgcgtg gaatatcgcg
     5461 tggcgggtgc cgatgccaac ccatatctgg tgatggcagc gatttttgcc ggtattttgc
     5521 atggccttga taacgagctg ccgttgcagg aagaagtcga aggcaacggg ctggaacagg
     5581 aaggcttacc cttcccgatt cgccagagcg atgccttggg tgagtttatc gagaatgatc
     5641 acctgcgccg ctatttaggc gaacggtttt gccatgtgta tcacgcctgt aaaaatgatg
     5701 agctgttgca gtttgagcgc ctcatcacag aaacagaaat tgagtggatg ttgaaaaacg
     5761 cgtaattccc catgcggggt tgtttcatcg gcctgagcgg agccaggcct gacatttgct
     5821 ggtaatccag acatttcccg gatgtcgcgt tatggagcgc aggcggcaac gggcttccgt
     5881 taacgattaa gactgtgacg aggaaatgag atgatgccga tgattaaatc cccgcatggc
     5941 gagggaggct gcgtatgcgc ccctcctgct actgactgga ctccgccccc tttgcttccc
     6001 ttgctaaaca ggtttgattt caggtcgaca cgaccgcaaa ccttattacg caggggaggc
     6061 agcaattatg gctattaatt caccactgaa tattgctgcg caacccggca aaacccgtct
     6121 gcgaaaatca ctgaaattgt ggcaggtggt aatgatgggt ctggcctatc tcacgccgat
     6181 gaccgtattt gatacttttg gtattgtctc cggcattagc gacggtcacg ttccagcgtc
     6241 ctatttgctg gcgctggcgg gcgtgctgtt taccgctatc agctacggca aactggttcg
     6301 ccagtttccg gaggccggtt cggcctatac ctacgcgcaa aagtcgatta atccgcacgt
     6361 cggatttatg gtcggctggt catcactgct ggattatctc tttttgccaa tgatcaacgt
     6421 tttgttggcg aaaatctatc tctccgccct cttcccggaa gtgccgccgt gggtgtgggt
     6481 ggtaaccttc gtcgccattt taaccgccgc gaatctgaag agcgtcaacc tggtcgctaa
     6541 cttcaatacc ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta tcttcctggt
     6601 ggttcaggga ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc agccgtttat
     6661 cagcgagaac gcgcacctga tcccgattat taccggtgcg acgattgtct gtttctcgtt
     6721 cctcggtttc gatgcggtga ccacgctttc ggaagagacg ccggacgccg cacgcgtgat
     6781 cccgaaagcc atcttcctga cggcggtata cggcggcgtt atctttatcg cggcgtcgtt
     6841 ctttatgcag ctgttctttc ccgatatcag ccgctttaaa gacccggacg ccgcactgcc
     6901 tgaaattgcg ctgtacgtcg gcggtaagct gttccagtcg attttccttt gcaccacgtt
     6961 tgtgaacacg ttagcgtctg gcctggcctc acatgccagc gtgtcacgtc tgctgtatgt
     7021 gatggggcgc gacaatgtgt ttccggagcg cgtgtttggc tatgtgcatc cgaaatggcg
     7081 gactccggca ctgaacgtca ttatggtcgg gattgtcgcg atgtcggcgc tgttcttcga
     7141 tttagttacc gccacagcgt taattaactt cggcgcgttg gtagcgttta ccttcgttaa
     7201 cctgtcggtg ttcaaccact tctggcggcg caaaggaatg aataaaagct ggaaggaaca
     7261 cttccactat ttattaatgc cgctggttgg cgcgctgacg gtgggcgtgc tgtggattaa
     7321 cctcgaagcc acctcgctga cgctgggtct ggtatgggct tcgttaggcg gcgcatattt
     7381 gtggtatctg atccgccgct atcgcaaagt gccgctgtac gaaggtgaca gaacgccagt
     7441 gagcgaaacg taatagtcgg atgcgcgccg cattatcctg cctgagggta atgcgccgcg
     7501 ccattaacta taaaaagatg tatgacccgg atcacgagtg ctatcatccg cccttatgct
     7561 tagcgcatta accccagggg caaataatga accacgatat cccgctaaaa tattttgata
     7621 ttgccgatga gtacgcgacc gaatgcgcag agcctgtcgc agacgctgaa cgcacaccgc
     7681 tggcccacta cttccagtta ctgctcaccc gtttgatgaa caatgaagag atcagcgaag
     7741 aagcccaaca tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt gatgagatcg
     7801 cagagttttt gaatcaatgg ggtaatgagt aattatttaa ttgcagacga tcggtcagta
     7861 tcgttgtccg gataaagcgt tcacgccgca tccggctaac aatgacacgc actgcagcgt
     7921 gctttaatgg gtttctcacg atcgtaccgc tctgctcctc gctaagttgt tctgtgagtt
     7981 atagttatag agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca cctgttcaac
     8041 gaccaaccac acggccctca ttacgacccc tgaacacaaa cggtaatggc aaatgtcatt
     8101 aaatattagt aagatagcct ctttacataa aatcccttaa tattatgcgc caggtattat
     8161 cgtctctttt ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg cctgaatccc
     8221 ccccgcatgc tgatatccgc gacagcggtt ttgtctattg cgtcagcggg caagtcaaca
     8281 cctttaaccc atccaaagcg agcagtgggt taattgtcga tacccttgcc gcccagtttt
     8341 atgatcgact gctggatgtc gatccctata cctatcgcct gatgccggaa cttgccgaaa
     8401 gctgggaagt actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc gatgttccgt
     8461 ttcaaaaaac cgactggttt actcccactc gtaaaatgaa tgccgacgat gtggtgttta
     8521 ccttccagcg aatttttgac cgcaacaacc cgtggcataa cgtcaacggc agcaacttcc
     8581 cctacttcga cagcctgcaa tttgccgata acgtgaaaag cgtccgcaaa ctggataatc
     8641 ataccgttga gttccgactc gcccagccgg atgcttcttt tttgtggcac ctcgcaaccc
     8701 attatgcttc ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa gatcgccagg
     8761 agcaactcga ccgtcaaccg gtcggcaccg gtccgtatca gttgtcggaa taccgcgccg
     8821 ggcaatttat tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg ttaatgccgc
     8881 aggtagtggt ggatttaggc tccggcggca ccggacgtct gtcgaaactc ctgaccgggg
     8941 aatgcgacgt tctggcctgg cctgctgcca gccagctatc cattttgcgt gacgacccgc
     9001 gcttgcgttt aacgctgcgt cctgggatga acgtcgccta tctggcattt aacaccgcca
     9061 aaccgccgct aaataatccc gctgtccgcc atgcgctggc actggcgatt aataaccagc
     9121 gcctgatgca atccatctat tatggtacgg ctgaaacggc ggcctctatt ttaccgcgcg
     9181 cctcgtgggc ctatgacaac gaggctaaaa ttactgaata caatccggcg aaatcgcgcg
     9241 aacagttgaa gtcgttgggg ctggaaaatt taacgctgaa actgtgggtg cccacacgtt
     9301 cgcaggcgtg gaaccccagt ccactgaaaa ctgccgaact gattcaggcg gatatggcgc
     9361 aggttggcgt aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag gcgcggttga
     9421 tggatatgag ccatgatctg acgttatccg gttgggcgac ggacagtaac gacccggaca
     9481 gtttcttccg tccgttactg agctgcgcgg caattcattc gcagaccaac ctcgcccact
     9541 ggtgcgatcc gaaattcgac agcgtattgc gtaaggcgct ctcttcgcag cagctggcgg
     9601 cgcgtattga agcctatgac gaagcgcaaa gtattctggc gcaggaattg cccattttgc
     9661 cgctggcgtc gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt ctggtactta
     9721 gcccgtttgg taacgcctcc tttgctgggg tgtatcgcga gaaacaggat gaggtgaaaa
     9781 agccatgatt atcttcacct tacgccgcat tttgttattg attgttacct tgttcctgct
     9841 gaccttcgtt ggcttcagtt tgagctattt cactccacac gcgccgttac aaggcgcgtc
     9901 actgtggaat gcctgggtgt tctggtttaa tggcctgatc cactgggatt ttggtgtttc
     9961 cagcattaac ggtcaaccaa ttgccgaaca gttaaaagag gtcttcccgg cgacgatgga
    10021 actgtgcatc ctcgccttcg gttttgcgct gattgttggc atccccgtgg ggatgattgc
    10081 cgggattacg cgccataagt ggcaggataa cttgatcaac gccatcgcct tgctgggctt
    10141 ttcaatcccg gtgttctggc tggcgcttct gttgacgctg ttttgttcac tcacgctagg
    10201 ttggttgccc gtttcagggc gtttcgatct gctctacgaa gtgaaaccga ttaccggttt
    10261 tgcgttgatt gatgcctggc tttcggactc accatggcgg gatgaaatga tcatgagcgc
    10321 aatccgccat atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa ctgaagtgat
    10381 ccgcctgatg cgtatcagca ctatcgaagt gtatgaccag aactatgtaa aagcggcggc
    10441 gacccgcggt ttgtcgcgct ttactatttt gcgtcgccac gttctgcata acgcgttacc
    10501 gccggttatt cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg cgatgatcac
    10561 cgaaatggtt tttagctggc cgggtttagg gcgctggtta attaacgcca tccgccagca
    10621 ggattacgca gccatttccg ccggagtgat ggtgtgtggt tcactggtca ttattgttaa
    10681 cgtgatttct gatattttgg gtgccatggc taaccctctg aaacataagg aatggtatgc
    10741 cttacgatag cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc gcctggcgca
    10801 aattttatag tgacgcctct gcgatggtcg gcctgtacgg ctgcgcgggg ctggctgtac
    10861 tgtgtatttt tggcggctgg tttgcgcctt acggcatcga ccagcaattt ctcggttacc
    10921 aattattgcc gccgtcatgg tcgcgctatg gcgaagtttc tttcttcctg gggactgacg
    10981 acctcggacg cgatgtttta agtcgtttac tgagcggagc tgcgccaacc gtgggtggcg
    11041 catttgtggt gacgcttgcc gcgacgatct gtggcctggt actgggcacc ttcgccgggg
    11101 cgacgcacgg cctccgctca gcggtgctta accatattct ggatacctta ctggcgatcc
    11161 cttcgctgct gctggcaatt atcgttgtag cgtttgccgg accgagtttg tctcacgcca
    11221 tgtttgccgt ctggctggcg ctgctgccgc gtatggtacg ttcgatttac agcatggtgc
    11281 atgacgaact ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca tcaacgctga
    11341 atattctctg gtttgctgtg atgcctaaca tcaccgccgg gctggtcacg gagatcaccc
    11401 gcgcactgtc gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat ctcggcgcac
    11461 aactcccctc gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg atttatgtcg
    11521 ccccgtggac tgtcatgctg ccaggtgcgg caattatgat tagcgtgctg ttggttaacc
    11581 tccttggcga tggcgttcgt cgtgcgatta ttgcgggggt ggaataatgc cattactcga
    11641 tattcgtaac ctgactattg aatttaaaac cggggatgag tgggttaaag ccgttgaccg
    11701 ggtaagtatg accttaaccg aaggtgaaat ccgcggtctt gttggtgaat ccggttccgg
    11761 caagagtttg attgcgaaag cgatttgtgg ggtgaataaa gataactggc gtgttactgc
    11821 tgaccgtatg cgttttgatg atatcgattt gctgcgtctc tccgcacgcg aacggcgcaa
    11881 actggttggc cataacgtgt cgatgatttt ccaggaaccg cagtcgtgtc ttgacccttc
    11941 agaacgtgtg ggtcgccagt tgatgcaaaa catcccagcc tggacctata aaggccgttg
    12001 gtggcagcgt tttggctggc gcaaacgccg tgccatcgaa ctactgcacc gcgtggggat
    12061 taaagatcac aaagatgcta tgcgcagttt tccctatgag ttaaccgaag gtgaatgtca
    12121 aaaagtgatg atagccattg cactggcgaa tcaaccgcgt ctgctaattg ctgacgaacc
    12181 gaccaactca atggagccaa caacccaggc gcaaatcttt cgcctgttga cgcgtctcaa
    12241 ccaaaacagc aataccacta ttttgcttat cagccatgac ttacaaatgc ttagccaatg
    12301 ggcggataaa attaacgtgc tttactgcgg tcaaacggtg gaaaccgcgc caagtaagga
    12361 gttggtgaca atgccacatc atccttatac ccaggcgctg atccgggcga taccagactt
    12421 cggtagcgcg atgccgcata aaagtcgcct caatacgctg cccggcgcta tcccactgct
    12481 ggaacagtta ccgattggtt gtcgtctggg gccgcgttgc ccgtacgcac aacgagaatg
    12541 cattgtgacg ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc atttcccgct
    12601 gaacatggag aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat ttaagtaaaa
    12661 cgttccgcta ccggaccggt tggtttcgtc gtcagaccgt agaagcggta aaacccttga
    12721 gctttacgct acgtgaaggc cagacactgg cgattattgg cgagaatggt tcgggtaaat
    12781 ccacgctggc aaaaatgctg gcgggaatga tagaacctac cagcggcgag ttattgattg
    12841 acgatcatcc actgcatttt ggcgattatt ccttccgcag tcagcgtatt cgcatgattt
    12901 ttcaggatcc ttcgacctcg ttgaatcccc gtcaacgtat ctcgcaaatt ctggattttc
    12961 cactgcgcct gaacaccaac ctggaaccgg aacagcgtcg caaacagatt attgaaacga
    13021 tgcgtatggt tgggctgcta ccggatcacg tcagttacta tccgcatatg ttggcccccg
    13081 ggcaaaaaca gcgtctgggt ctggcgcgcg cgctgatatt gcgcccaaaa gtcattattg
    13141 ccgatgaagc cctcgcttca ctggatatgt cgatgcgttc gcagttgatt aatctgatgc
    13201 tggaattaca ggaaaaacag ggcatttcgt atatttatgt tacccagcat atcggaatga
    13261 tgaagcacat tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc gagcgaggca
    13321 gcaccgcgga tgtgctggca tcgccgctgc atgaactcac caaacggctg attgccgggc
    13381 actttggcga agcattgaca gcggatgcgt ggcgtaaaga tcgctaattt tctgaatcct
    13441 ccttccagat tatccacctc attctgacaa gagattaaat cttgtcagaa tgaggtgatg
    13501 gataaaataa aatcaaatgc gcgtgattta agacgcaatc tcactttgca ggaacgaaag
    13561 ctctggcgat atcttcgcag ccgacgtttt ggtgatttca aatttcgccg tcaacatccg
    13621 gtggggagct acattctcga ttttgcttgc tgctcggcgc gcgtagtcgt tgagctggat
    13681 ggtgggcagc atgatttggc agttgcctat gataccaggc gcactagctg gcttgagtcg
    13741 cagagctgga ccgtgctgcg tttctggcat aacgagattg attgtaatga ggaggcggtg
    13801 ctggagatta ttctgcagga actgaaccgc cggtcaccct caccctgaaa gggcgagggg
    13861 gccgaccgag ccgaatagct gttgtggtga aaacatggag acagtgctgg agaatattcg
    13921 gcaaggtctg aaccgtccca gccatcgccc tgaaagggtt agggggctgt ataagcctgt
    13981 ttgttgctgg ggtgacaata tttgcacaat atggtcccct cgcccctctg gggagaggat
    14041 tagggtgtgg ggaaaagcgc ccaccctgcc cgcaccctgc tccggtcgga cctggcagct
    14101 atagccactc acagccaggt tgattataat aaccgtttat ctgttcgtac tgtttactaa
    14161 aacgacgaat cgcctgattt tcaggcacaa caagcatcaa caataaggat taaagctatg
    14221 ggttttcttt ccggtaagcg cattctggta accggtgttg ccagcaaact atccatcgcc
    14281 tacggtatcg ctcaggcgat gcaccgcgaa ggagctgaac tggcattcac ctaccagaac
    14341 gacaaactga aaggccgcgt agaagaattt gccgctcaat tgggttctga catcgttctg
    14401 cagtgcgatg ttgcagaaga tgccagcatc gacaccatgt tcgctgaact ggggaaagtt
    14461 tggccgaaat ttgacggttt cgtacactct attggttttg cacctggcga tcagctggat
    14521 ggtgactatg ttaacgccgt tacccgtgaa ggcttcaaaa ttgcccacga catcagctcc
    14581 tacagcttcg ttgcaatggc aaaagcttgc cgctccatgc tgaatccggg ttctgccctg
    14641 ctgacccttt cctaccttgg cgctgagcgc gctatcccga actacaacgt tatgggtctg
    14701 gcaaaagcgt ctctggaagc gaacgtgcgc tatatggcga acgcgatggg tccggaaggt
    14761 gtgcgtgtta acgccatctc tgctggtccg atccgtactc tggcggcttc cggtatcaaa
    14821 gacttccgca aaatgctggc tcattgcgaa gccgttaccc cgattcgccg taccgttact
    14881 attgaagatg tgggtaactc tgcggcattc ctgtgctccg atctctctgc cggtatctcc
    14941 ggcgaagtgg tccacgttga cggcggtttc agcatcgctg caatgaacga actcgaactg
    15001 aaataatcgt tctgttggta aagatgggcg gtgttctgcc gcccgttatc tccgttatac
    15061 ctttctgata tttgttatcg ccgatccgtc tttctcccct tcccgccttg cgtcaggata
    15121 acgatttcct ttacgaccaa ggagcgccca tggaacaacg ccacatcacc ggcaaaagcc
    15181 actggtatca tgaaacgcaa tccagtactg cggaatatga cgttctgcct ctggtcccgg
    15241 aagccgcaaa ggtcagcgat ccctttctgc tcgacgtgat ccttgatgaa gaaacgctgg
    15301 cccccttcct ttcatggctg gtccctgcgc gcgttcttgc agtggagttg ttccctgacc
    15361 agcttaccgt gacccgttca caaaccttca ccgcttatga acgcttgtcg acggccctga
    15421 cggttgctca ggtttgcggc gtccagcggt tatgtaacta ctattcggcg cgacttacgc
    15481 cgctccccgg acctgattcc tccagggaaa gtaatcatcg gttggcacaa atcacgcaat
    15541 atgcccgcca actggctagc tcgccttcta ttatcgacaa ccgatcgcgc cagcatctga
    15601 atgacgtcgg tcttactgcc tgggactgtg tgatcattaa ccaaatcatt ggttttattg
    15661 gctttcaggc gcggacaatt gcgacatttc aggcttatct caggcatccg gtacgctggt
    15721 tacccgggct ggagatacaa aactacgccg acgcgtcact gtttgctgat gaatcattac
    15781 gctggcgaag cagctatgag gtggaaaaac tacctgaaga gcacacaaaa agttcaactg
    15841 cagaactttg ccaactggcc gaaatactct ctctccaccc tatttcactt tcccttctcg
    15901 aaaagttgtt aaacagcaca cgggtcaata cacagccgga taatcagctt gcggcgttgt
    15961 tatgcgcgcg tataaatggc agtcctgctt gttttgccac ctgtatggat tcatcaaatg
    16021 aatataaaaa aatcagcacc cttatgcgca agggcgaaaa tgaaattaac caatgggctg
    16081 accgtcattc tgttgagcac gctaccgttc aggcgataca atggctgacc cgagcacccg
    16141 atcgctttag cgccgcccag ttcagccctt tactcgaaca cgaaaaatca tcaacgcaga
    16201 ttattaatct gctggtatgg agcgggctgt gtggctggat aaatcgctta aaaatcgcgt
    16261 tgggtgagac atattaacct tgccgcgtca gacagattcg cgtaaaactg tcagccgctc
    16321 taatggccac caaaatagac aattatgttt caggacaacc cgctgctagc gcagcttaaa
    16381 cagcaactgc attcccagac gccacgcgct gaaggggtgg taaaagccac agaaaaaggc
    16441 tttggcttcc tggaagtcga cgcgcaaaaa agttatttca ttccgccgcc gcagatgaaa
    16501 aaagtcatgc atggcgaccg aattatcgcg gtgatccaca gtgaaaaaga acgtgaatcc
    16561 gcagagccag aagaactggt tgaaccgttc ctgactcgtt tcgtgggtaa agttcagggc
    16621 aaaaatgacc gtctggccat cgttcctgat catccactct taaaagacgc cattccttgc
    16681 cgcgcagccc gtggcctgaa ccacgagttt aaagaaggcg actgggcggt tgccgaaatg
    16741 cgccgtcatc cgctgaaagg cgatcgttct ttctatgctg aactgacaca atacatcact
    16801 tttggtgacg accactttgt accgtggtgg gttacccttg cgcgccataa tctggaaaaa
    16861 gaagcacca
//
LOCUS       DAEVZA010000108        16523 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEVZA010000108 DAEVZA010000000
VERSION     DAEVZA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.108"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1226
                     /locus_tag="LAF92_003632"
     CDS             <1..1226
                     /locus_tag="LAF92_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBI9792571.1"
                     /translation="VVLTDNAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVV
                     QQVHKGNQAADFDTFGKGAWTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQR
                     ETITVLGYLFYIGDRTKTDLPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYG
                     FKDFKLKGGVLPGEQEIDTVRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTY
                     AEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHF
                     WTLSGAVRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEG
                     DCRLTKNPLEIKNGKIAVPDAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLI
                     PGWTFDRKRPVFGRH"
     gene            1247..2587
                     /gene="gudD"
                     /locus_tag="LAF92_003633"
     CDS             1247..2587
                     /gene="gudD"
                     /locus_tag="LAF92_003633"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBI9792572.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(2819..5575)
                     /gene="barA"
                     /locus_tag="LAF92_003634"
     CDS             complement(2819..5575)
                     /gene="barA"
                     /locus_tag="LAF92_003634"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBI9792573.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            5632..6933
                     /gene="rlmD"
                     /locus_tag="LAF92_003635"
     CDS             5632..6933
                     /gene="rlmD"
                     /locus_tag="LAF92_003635"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBI9792574.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            6981..9215
                     /locus_tag="LAF92_003636"
     CDS             6981..9215
                     /locus_tag="LAF92_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBI9792575.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            9293..9541
                     /gene="mazE"
                     /locus_tag="LAF92_003637"
     CDS             9293..9541
                     /gene="mazE"
                     /locus_tag="LAF92_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HBI9792576.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            9541..9876
                     /gene="mazF"
                     /locus_tag="LAF92_003638"
     CDS             9541..9876
                     /gene="mazF"
                     /locus_tag="LAF92_003638"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HBI9792577.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            9947..10738
                     /gene="mazG"
                     /locus_tag="LAF92_003639"
     CDS             9947..10738
                     /gene="mazG"
                     /locus_tag="LAF92_003639"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBI9792578.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            10966..12603
                     /gene="pyrG"
                     /locus_tag="LAF92_003640"
     CDS             10966..12603
                     /gene="pyrG"
                     /locus_tag="LAF92_003640"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBI9792579.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            12691..13989
                     /gene="eno"
                     /locus_tag="LAF92_003641"
     CDS             12691..13989
                     /gene="eno"
                     /locus_tag="LAF92_003641"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBI9792580.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(14049..14921)
                     /locus_tag="LAF92_003642"
     CDS             complement(14049..14921)
                     /locus_tag="LAF92_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBI9792581.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALARDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(14935..15075)
                     /locus_tag="LAF92_003643"
     CDS             complement(14935..15075)
                     /locus_tag="LAF92_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792582.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            15214..15885
                     /gene="queE"
                     /locus_tag="LAF92_003644"
     CDS             15214..15885
                     /gene="queE"
                     /locus_tag="LAF92_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBI9792583.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   16223..16497
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16223..16253
                     /rpt_unit_seq="gagtgttccccgcgccagcggggataaaccg"
BASE COUNT         4215 a         3888 c         4371 g         4049 t
ORIGIN      
        1 ttgtggtact caccgataac gccgggcata ccggcattgg tgaagcgccg ggcggagagg
       61 tgatttatca aacactggtc gatgctattc cgatggttct gggccaggaa gttgcgcgcc
      121 tgaataaagt ggttcagcag gtgcataaag gtaatcaggc agccgatttt gataccttcg
      181 gcaaaggtgc ctggactttt gaattgcgcg ttaatgccgt ggcggcgctg gaagccgcct
      241 tgcttgacct gctaggtaag gcgctgaatg ttccggtctg cgaactgttg gggccaggca
      301 agcaacgcga gactattacc gtcctcggtt atctgtttta tatcggtgat cggaccaaaa
      361 ccgatcttcc ttatctggaa aatacgccgg gcaaccatga gtggtatcag ttgcgccatc
      421 agaaagcgat gaacagcgaa gccgttgtgc gtctggcgga agcctcacag gatcgctatg
      481 gctttaaaga tttcaaactt aagggcgggg tgttacctgg cgagcaagaa atcgacactg
      541 ttcgtgcatt gaagaaacgc ttccctgatg cgcggattac cgttgatccc aacggtgcct
      601 ggctacttga tgaagccatt tctctatgca aagggctgaa tgatgttctt acctatgccg
      661 aagatccatg cggcgcagaa cagggtttct ccggtcgtga agttatggcg gagtttcggc
      721 gggcgaccgg cttgcccgtc gcgactaaca tgatcgccac caactggcgc gaaatgggtc
      781 atgcggtgat gctcaatgcg gtagatattc cacttgccga tccgcacttc tggacgcttt
      841 ccggtgcagt ccgtgtggcg cagctttgcg acgactgggg gctgacctgg ggctgccatt
      901 ctaataacca tttcgatatc tctctggcga tgtttaccca tgtgggcgcg gcggcaccgg
      961 gtaatcctac cgctatcgat acccactgga tttggcagga gggcgattgt cgcctgacca
     1021 aaaatccgct ggagattaaa aacggaaaaa ttgccgttcc tgatgcgccc ggtctgggcg
     1081 tggaactgga ctgggaacag gtacaaaagg cacatgaggc ctataaacgt ctgcctggcg
     1141 gtgcgcgtaa cgacgcaggt ccgatgcagt acctgatccc cggctggacc tttgaccgta
     1201 aacgtcccgt tttcggccgt cattgattct gaaaaaggac ataaatatga gttctcaatt
     1261 tacgacgcct gttgttactg aaatgcaggt catcccggtg gcgggtcatg acagtatgct
     1321 gatgaatctg agtggtgcac acgcaccgtt ctttacgcgt aatattgtga ttatcaaaga
     1381 taattctggt cacactggcg taggggaaat tcccggcggc gagaaaatcc gtaaaacgct
     1441 ggaagatgcg attccgctgg tggtaggtaa aacgctgggt gaatacaaaa acgttctgac
     1501 gctggtgcgt aatacttttg ccgatcgtga tgctggtggg cgcggtttgc agacatttga
     1561 tttgcgtacc actattcatg tagttaccgg gatagaagcg gcaatgctgg atctgctggg
     1621 gcagcatctg ggggtaaacg tggcatcgct gctgggcgat ggtcaacagc gtagcgaagt
     1681 cgaaatgctc ggttatctgt tcttcgtcgg tgatcgcaaa gccacaccgc tgccgtatca
     1741 aagccagccg gatgactcat gcgactggta tcgcctgcgt catgaagaag cgatgacgcc
     1801 ggatgcggtg gtgcgcctgg cggaagcggc gtatgaaaaa tatggcttca acgatttcaa
     1861 actgaaaggc ggtgtactgg ccggggaaga agaggccgag tctattgtgg cactggcgca
     1921 acgcttcccg caggcgcgta ttacgctcga tcctaacggt gcctggtcgc tgaacgaagc
     1981 gattaaaatt ggtaaatacc tgaaagggtc gctggcttat gcagaagatc cgtgtggtgc
     2041 ggagcaaggt ttctccgggc gtgaagtgat ggcagagttc cgtcgcgcaa ctggcctgcc
     2101 gaccgcaacc aatatgatcg ccaccgactg gcggcagatg ggccatacgc tctccctgca
     2161 atccgttgat atcccgctgg cggatccgca tttctggacg atgcaaggtt cggtacgtgt
     2221 ggcgcaaatg tgccatgaat ttggcctgac ctggggttca cactcaaaca accacttcga
     2281 tatttccctg gcgatgttta cccatgttgc cgccgctgca ccgggtaaaa tcaccgcgat
     2341 tgatacgcac tggatttggc aggaaggcaa tcagcgcttg accaaagaac cgtttgagat
     2401 caaaggcggt ctggttcagg tgccggaaaa accggggctg ggtgtagaaa tcgatatgga
     2461 tcaagtgatg aaagctcatg agctgtatca gaaacacggg cttggcgcgc gtgacgatgc
     2521 gatgggaatg cagtatctga ttcctggctg gacgttcgat aacaagcgcc cgtgcatggt
     2581 gcgttaagac agaaaaccaa aaccggctct atctggagcc ggcttgagac tgatgctaaa
     2641 cgcaaaatag cctgttgtgc tatgtatcag gtctacatca ttttttaatt cattgaattt
     2701 aattggtttt gtaggccgga taaggcgtta acgccgcatc cggcataaac acaggcactt
     2761 tgtcaccaat ctgaaaccag cgtcataaaa agccgattgc tactcgacaa gacgtccgtt
     2821 acccgagaat tttgctggct tcgcgcgcta cattatccat ctcgtccaac agttccagca
     2881 gctccggctc caaatcttct tctttagtac cgctacgtag ctgttgttcg atcagttggc
     2941 agagattctt catacgcggc acaccgctat agccgcaact gccatgcagt ttatgaatca
     3001 aatcaaccag gccttccggg ttttctccaa ccagctgttc ctcaactttg ttgcgaactt
     3061 caggcaggaa atcgagtaac atttgcagca tatcgcgcgc taaatcggtt tttcctgctg
     3121 cctggcgtag tgccagttgc cagtcgaggg tcgcattcgg gttcaccaca atttcgttga
     3181 cttcgggcgt cacgacgcga gaggaaatac cgctgccagg cttgtagcgc aacaacaaat
     3241 tatgcaatcg ctcttcttca atcggtttcg ccagataatc gctcatccct gcgccaagca
     3301 gcttctcttt ttgcccggcc attgcatgcg ccgttaccgc gataaccggc gtttgttgct
     3361 gatgggggag ctggtggatg agctcgcagg cccgaatgcc atccatgtca ggcatttgaa
     3421 tatccattaa gatcaaatcg aacggcatct gtttcgcccg ttcaaccgcc tgatgcccgc
     3481 tatcgcaaag ttccacatgt tgcaccatat cttccagcaa tgcgccgata agtttcaggt
     3541 tagcggggtt gtcatcaact gccatgactg tcattgccag cttgctttca tcggttacag
     3601 gcaaaagcgt gttttgtttg tgatgacaaa attccgtcag ggcaggcaac aggcgcgtag
     3661 gtgttaatgg tttcagcaga cacgcgccga taccatcttg cttgagtttt tctgcattga
     3721 cttgtgcatg gcaaggaagt gccagcatca ggaaatcggt catcgatacc gctttcgcta
     3781 atcgctcatg ttgcattgtt agcggctcgc ggaaggtcac cgcgatgcct aacaacatca
     3841 tgtcgtaatg cgcgggaggc agcgcggaga acgttgggct ataaaccact tccagcggcg
     3901 tttcacttaa aatatccagc gtgcattgcg ctgctgcgga gtttggttcg acataggcca
     3961 ggcgcttacc ggcgaggcac tgggtggatg gcccttcgat aataatgttc gggttcagat
     4021 cgagattaat gtggaaccag aaagttgaac cgcgattcgg ctggctatgg aacgaaatat
     4081 cgccgcccat ttcattaacc agtttttgtg taatcaccag ccccagaccg gtgccaccat
     4141 gacggcggga aatactggca tcagcctgtc ggaaggcctg gaataagcgc gattgatcgc
     4201 gttcaggaat accaatgccg gtatcccgaa tctgcacttc aatctgcact ttggtattac
     4261 tcagcgcacg tttttctacc agaatatcaa tgttgccatt ctcggtgaat ttaattgcat
     4321 tccccaccag gttagtgatg atttgctgta atcgcagtgg gtcgccgatc acgttatcag
     4381 gcacgtcgct tttaatattg agcgtcagtt ccaacccttt atcgtgagaa gaatgtgcca
     4441 gcagagtaac gacttcatcc agcgtgctgc gtagtgggaa tggaatactt tccagaatca
     4501 gcttacctgc ttccagtttc gagaagtcga gaacatcatt aataattgcc agcaaattat
     4561 ttgccgaacg ttcaatcgta ttcaggtgat cgcgctgcgt tggtgttaat tctgttttca
     4621 gcgtcaggcg ggtaaagcca ataacaccat tcagtggtgt acgcagctcg tgtgacatat
     4681 ttgccagaaa ctcggattta atacgcgccg cttcctgggc gcgctttttc gccagatcta
     4741 actcaacgtt ctgaatttcc atctgctcca gcgtttcacg cagatcggac gtcgcctggt
     4801 cgatattgtg ctgcatctct tcgtgataag cagccagcga cattgccatc gagttgatac
     4861 cgtttttcag catatccagc tcgccgagca taaatccttc cacccggctg tcgagttgcc
     4921 cgcgacggat gcggtcgacg gtattcacca tgttgcgaat cggaccggtt acatcgcgca
     4981 ttaagcgcca gccaaaaata agcgcaatac cgatacaaaa cagcatcatc acgctggaaa
     5041 taaagatctc tttatattgc tgcaagcgaa ccgatttaag atccagctca agcgcaatat
     5101 atcccaacat attttgacta tttttggcat cgctactggg cgattcgtcg ggggagtaac
     5161 tctcagaaat aatcggcgtg cggaggatca taatatcgcc gtcacgagtg acagtgagct
     5221 ggcgaggaaa cggcacgttg ctgccgagct gcattgatga gggatcaaga tgaaaattgg
     5281 aggtgacaaa gagtcggtta ttttcatcat aaaccgaaat cgcgcgaaca atatcggaat
     5341 ggcgacgatg cagtacgctt attaactgac cgatagattc gcgattttgc aggctcatgc
     5401 catattcagt agaaactgca agcggctcaa taatgctggc accggcatct tccagttgac
     5461 gctgcaagtc gttatagcga tgcacgacga aaaagatact cagcaataaa ccaataagga
     5521 cggtcggtgc caggatcaga atcatcatgc gtgcgcgcag gctgtagttg gtcatggagt
     5581 tccgttatgg gacaattaag gtcacactgt taaattgaga aagtctcgcc aatggcgcaa
     5641 ttctactctg caaaacgacg cacgacgacg cgtcagatca taaccgtttc agtcaacgac
     5701 ctcgactctt ttggtcaggg cgtggcgcga cataacggca aagcgttatt tattcctgga
     5761 ttactgccac aggaaaacgc ggaagttact gttactgaag ataaaaaaca gtacgcccgc
     5821 gctaaagtcg tgcgccggtt aagcgatagc ccggaacgcg aaacgccacg ctgccctcat
     5881 tttggcgtat gcggtggctg tcagcaacaa cacgccagcg tggatttaca gcagcgaagc
     5941 aaaagtgcgg cactcgcccg attaatgaaa cacgaagtct ctgaagtgat cgccgatgtt
     6001 ccctggggct atcgccgtcg cgcgcgttta agtttgaact acttaccgaa aacacagcaa
     6061 cttcagatgg gctttcgcaa agcgggctcc agtgacattg tcgacgtcaa acaatgcccc
     6121 attttagtgc cccaacttga agcattgctg cccaaagtca gggcatgcct gggcagctta
     6181 caagctatac gccatcttgg tcatgttgaa ctggtacagg caaccagcgg cacgctgatg
     6241 attttgcgcc ataccgcacc gctaagttcg gctgatcgcg aaaaactgga acgcttttcg
     6301 cattctgaag gcctggatct gtatctcgcc cccgatagtg agatactcga aaccgtctct
     6361 ggtgagatgc cctggtatga ctcaaacggg ttgcgcttaa cttttagccc gcgcgatttt
     6421 attcaggtca atgcgggtgt gaaccaaaaa atggtagcgc gtgcgttgga atggctggat
     6481 gtgcaacctg aagatcgcgt actggatctg ttctgcggta tgggcaactt tacactgcca
     6541 ttggcgacac aagctgccag tgtggtgggt gtagaaggtg ttccggcgct ggtggaaaaa
     6601 ggccagcaga atgcgcgtct taatggttta cagaatgtga cgttttatca cgaaaatctt
     6661 gaagaagatg tcacaaagca gccgtgggcg aaaaacggct tcgataaagt gttgctggac
     6721 ccggcgcgag caggtgccgc aggtgttatg cagcaaatta taaaactgga acctattcgt
     6781 atagtttatg tatcctgtaa ccctgcaacg ctggctcggg atagcgaagc gttattaaaa
     6841 gcaggatata ccattgcgcg actggcgatg ctggatatgt tcccacacac gggacatctg
     6901 gaatcgatgg tacttttctc gcgcgttaaa tagttacgat ttgccgattt cggcaggtct
     6961 ggtccctaaa ggagaggaca atggttgcgg taagaagtgc acatatcaat aaggctggtg
     7021 aatttgatcc ggaaaaatgg atcgcaagtc tgggtattac cagccagaag tcgtgtgagt
     7081 gcttagccga aacctgggcg tattgtctgc aacagacgca ggggcatccg gatgccagtc
     7141 tgttattgtg gcgtggtgtt gagatggtgg agatcctctc gacattaagt atggacattg
     7201 acacgctgcg ggcggcgctg ctgttccctc tggctgatgc caacgtagtc agcgaagatg
     7261 tgctgcgtga gagcgtcggt aagtcggtcg ttaaccttat tcacggcgtg cgtgatatgg
     7321 cggcgatccg ccagctgaaa gcgacgcaca ctgattctgt ttcctccgaa caggtcgata
     7381 acgttcgccg gatgttattg gcgatggtcg atgattttcg ctgcgtggtc atcaaactgg
     7441 cggagcgtat tgctcatctg cgcgaagtaa aagatgcgcc ggaagatgaa cgcgtactgg
     7501 cggcaaaaga gtgcaccaat atctacgcgc cgttggcgaa ccgtcttggg attgggcaac
     7561 tgaaatggga gctggaagat tactgcttcc gttatctcca cccgaccgaa tacaaacgca
     7621 tcgcaaaact gctgcatgaa cgccgtctcg accgcgaaca ctatatcgaa gagtttgtcg
     7681 gccatctgcg cgctgagatg aaagctgaag gcgttaaagc tgaagtgtat ggtcgtccga
     7741 aacacatcta cagtatctgg cgcaaaatgc agaaaaagaa cctcgccttc gatgagctgt
     7801 ttgatgtgcg tgcggtacgt attgtcgccg agcgtttaca ggattgttat gccgcactgg
     7861 ggatagtgca cactcactat cgccacctgc cggatgagtt tgacgattat gtcgctaacc
     7921 cgaaaccaaa cggttatcag tctattcata ccgtggttct ggggccaggt ggcaaaaccg
     7981 ttgagatcca aatccgcacc aaacagatgc atgaagatgc agagttgggt gttgctgcgc
     8041 actggaaata taaagagggc gcggcagctg gcggcgcacg ttcgggacat gaagaccgga
     8101 ttgcctggct gcgtaaactg attgcgtggc aggaagagat ggctgattcc ggcgaaatgc
     8161 tcgacgaagt acgcagccag gtctttgacg accgggtgta tgtctttacg ccgaaaggtg
     8221 atgtcgttga tttgcctgcg ggatcaacgc cgctggactt cgcttaccac atccacagtg
     8281 atgtcggaca ccgctgcatc ggggcaaaaa ttggcgggcg cattgtgccg ttcacctacc
     8341 agctgcagat gggcgaccag attgaaatta tcacccagaa acagccgaac cccagccgtg
     8401 actggttaaa cccaaacctc ggttacgtca caaccagccg tgggcgttcg aaaattcacg
     8461 cctggttccg taaacaggac cgtgacaaaa acattctggc tgggcggcaa atccttgacg
     8521 acgagctgga acatctgggg atcagcctga aagaagcaga aaaacatctg ctgccgcgtt
     8581 acaactttaa tgatgtcgac gagttgctgg cggcgattgg tggcggggat atccgtctca
     8641 atcagatggt gaacttcctg caatcgcaat ttaataagcc gagtgccgaa gagcaggacg
     8701 ccgccgcgct gaagcaactt cagcaaaaaa gctacacgcc gcaaaaccgc agtaaagata
     8761 acggtcgcgt ggtggtcgaa ggtgttggca acctgatgca ccacatcgcg cgctgctgcc
     8821 agccgattcc tggagatgag attgtcggct tcattaccca ggggcgcggt atttcagtac
     8881 accgcgccga ttgcgaacaa ctggcggaac tgcgctccca tgcgccagaa cgcattgttg
     8941 acgcggtatg gggtgagagc tactccgccg gatattcgct ggtggtccgc gtggtggcta
     9001 atgatcgtag tgggttgtta cgtgatatca cgaccattct cgccaacgag aaggtgaacg
     9061 tgcttggcgt tgccagccgt agcgacacca aacagcaact ggcgaccatc gacatgacca
     9121 ttgagattta caacctgcaa gtgctggggc gcgtgctggg taaactcaac caggtgccgg
     9181 atgttatcga cgcgcgtcgg ttgcacggga gttaggccga aatttgctcg tatctacaat
     9241 gtagattgat atatactgta tctacatatg atagcggttt gaggaaaggg ttatgatcca
     9301 cagtagcgta aagcgttggg gaaattcacc ggcggtgcgg atcccggcta cgttaatgca
     9361 ggcgctcaat ctgaatattg atgatgaagt gaagattgac ctggtggatg gcaaattaat
     9421 tattgagcca gtgcgtaaag agcccgtatt tacgcttgct gaactggtca acgacatcac
     9481 gccggaaaac ctccacgaga atatcgactg gggagagccg aaagataagg aagtctggta
     9541 atggtaagcc gatacgtacc cgatatgggc gatctgattt gggttgattt tgacccgaca
     9601 aaaggtagcg agcaagctgg acatcgtcca gctgttgtcc tgagtccttt catgtacaac
     9661 aacaaaacag gtatgtgtct gtgtgttcct tatacaacgc aatcaaaagg atatccgttc
     9721 gaagttgttt tatccggtca ggaacgtgat ggcgtagcgt tagctgatca ggtaaaaagt
     9781 atcgcctggc gggcaagagg agcaacgaag aaaggaacag ttgccccaga ggaattacaa
     9841 ctcattaaag ccaaaattaa cgtactgatt gggtagtgtt actaactcac ttctattctg
     9901 gtcacaggtt tccacctgac agacccgcaa ttttcaggac aattcaatga atcaaatcga
     9961 ccgtttgctc actattatgc agcgcctgcg cgatccggaa aacggctgcc cgtgggataa
    10021 agagcagaca tttgccacca ttgcgcctta cacccttgaa gaaacctacg aagtgctgga
    10081 cgccatcgcc cgtgaagatt ttgacgatct tcgcggtgaa ctgggcgatc tgctattcca
    10141 ggtggtgttt tacgcgcaaa tggctcagga agaagggcgc tttgacttta atgatatttg
    10201 cgctgctatt agcgataaat tagagcgtcg ccatccgcat gtttttgctg atagttctgc
    10261 cgaaaacagt agtgaagtgc ttgcccgttg ggagcaaatc aaaaccgaag agcgcgcgca
    10321 gaaagcgcag cattcggcgc tggacgatat tcctcgtagt ttaccggctt taatgcgtgc
    10381 gcaaaaaatc cagaaacgtt gcgccaacgt tggcttcgat tggacgacgc ttggtccggt
    10441 agtcgataaa gtctacgaag agatcgacga ggtgatgtac gaagcgcggc aggctgttgt
    10501 cgaccaggct aaactggagg aggaaatggg ggacctgctg tttgccacgg tcaatctggc
    10561 tcgtcattta gggacaaaag cggaaatcgc attgcaaaaa gcgaacgaaa aatttgagcg
    10621 tcgttttcgc gaagtggagc gtattgttgc cgcgcgtgga ctggaaatga caggtgttga
    10681 cctcgaaaca atggaagaag tctggcaaca ggtaaaacgg caggaaattg atctctaagg
    10741 gaattacgcg gtcaagcgcc atttgtgtca ttttttaaat gacaagcgct tgatttgcgt
    10801 caaaaacatt taccccaaag gggctatttt ctcactcctg atttcaatag tgcgctggcg
    10861 aagaggaggg ataatgaaag tttgtggcac aggtcatgtt cgggtatact gctttcccgt
    10921 cctggttatt ccatcgtctt ttcaacctaa cttctcaggt tcagcatgac aacgaactat
    10981 atttttgtga ccggcggggt cgtatcctct ctgggtaaag gcattgccgc agcctccctc
    11041 gcagccattc ttgaagcccg tggcctcaat gtgaccatca tgaaactgga tccgtacatc
    11101 aacgtcgatc caggtactat gagcccaatc caacacgggg aagtgttcgt tactgaagac
    11161 ggcgctgaaa ccgacctgga cctggggcac tacgagcgtt tcattcgtac caaaatgagc
    11221 cgccgcaaca acttcaccac gggtcgtatc tactctgacg ttctgcgtaa agaacgccgc
    11281 ggtgactacc tcggcgcaac cgtgcaggtt attccgcaca tcactaacgc aatcaaagag
    11341 cgcgtgctgg aaggtggcga aggtcatgac gtagtactgg tagaaatcgg cggtacagta
    11401 ggtgatatcg aatccttgcc gttcctcgaa gcgattcgcc agatggctgt tgaaattggc
    11461 cgtgagcaca ctctgtttat gcacctgacg ctggtgccgt acatggcagc gtctggtgaa
    11521 gtcaaaacca aaccgactca gcactctgta aaagagctgc tctccatcgg tatccagcct
    11581 gacatcctga tttgtcgttc agatcgcgct gttccggcga acgaacgtgc gaagattgca
    11641 ttgttctgta atgttccgga aaaagcggtt atttctctga aagacgtcga ttccatctat
    11701 aaaattccgg gcctgttgaa atctcagggg ctggacgatt atatttgtaa acgattcagc
    11761 ttaaactgcc cggaagcgaa tctgtccgaa tgggaacagg ttatcttcga agaagcgaac
    11821 ccggtaagtg aagtcaccat cggtatggtc ggcaagtaca ttgaactgcc ggatgcttat
    11881 aaatcagtga tcgaagcact gaaacacggt gggctgaaga atcgtgtcag cgtcaacatc
    11941 aaactgatcg attcacaaga tgttgaaacg cgcggcgttg aaatccttaa aggtctggac
    12001 gcaatcctag tacctggcgg ttttggctat cgtggcgtag aaggcatgat tacgaccgcg
    12061 cgttttgcgc gtgagaacaa tattccttat ctgggcattt gcctgggtat gcaggtggcg
    12121 ttaattgatt acgctcgcca tgttgccaac atggagaacg ccaactctac ggaatttgtg
    12181 ccagactgta agtacccggt tgtggcgctg attaccgagt ggcgcgatga aaacggcaac
    12241 gttgaagttc gtagcgagaa gagcgatctc ggcggtacca tgcgtctcgg cgcacagcag
    12301 tgccagttgg ttgacgatag cctggttcgc cagctgtaca atgcgccgac aattgttgag
    12361 cgtcatcgtc accgttacga agtcaacaac atgctgttga aacagattga agatgcaggt
    12421 ctgcgcgttg cgggccgttc cggggatgat cagttggtcg agatcatcga agttccgaat
    12481 cacccgtggt tcgtggcttg ccagttccat ccggagttta cttctactcc acgtgatggt
    12541 cacccgctgt ttgcaggctt tgtgaaagcc gccagcgagt tccagaaacg tcaggcgaag
    12601 taagtaaaaa agttagaacg gcaacgcgta ccctgggtac gcgttgtttg tctggagttt
    12661 cagtttaact agtgacttga ggaaaaccta atgtccaaaa tcgtaaaaat catcggtcgt
    12721 gaaatcatcg actcccgtgg taacccgact gttgaagccg aagtacatct ggagggtggt
    12781 ttcgtcggta tggcagctgc tccgtcaggt gcttctactg gttcccgtga agctctggaa
    12841 ctgcgcgatg gcgacaaatc ccgtttcctg ggtaaaggcg taaccaaagc tgttgctgcg
    12901 gtaaacggcc cgatcgctca ggcgctgatt ggcaaagatg ctaaagatca ggctggcatt
    12961 gacaagatca tgatcgacct ggacggcacc gaaaacaaat ccaaattcgg cgcgaacgca
    13021 atcctggctg tatctctggc taacgccaaa gctgctgcag ctgctaaagg tatgccgctg
    13081 tacgagcaca tcgctgaact gaacggtact ccgggcaaat actctatgcc ggttccgatg
    13141 atgaacatca tcaacggtgg tgagcacgct gacaacaacg ttgatatcca ggaattcatg
    13201 attcagccgg ttggcgcgaa aactgtgaaa gaagccatcc gcatgggttc tgaagttttc
    13261 catcacctgg caaaagttct gaaagcgaaa ggcatgaaca ctgctgttgg tgacgaaggt
    13321 ggctatgcgc cgaacctggg ttccaacgct gaagctctgg ctgttatcgc tgaagctgtt
    13381 aaagctgctg gttatgaact gggcaaagac atcactctgg cgatggactg cgcagcttct
    13441 gaattctaca aagatggtaa atacgttctg gctggcgaag gcaacaaagc gttcacctct
    13501 gaagaattca ctcacttcct ggaagaactg accaaacagt acccgatcgt ttctatcgaa
    13561 gacggtctgg acgaatctga ctgggacggt ttcgcatacc agaccaaagt tctgggcgac
    13621 aaaatccagc tggttggtga cgacctgttc gtaaccaaca ccaagatcct gaaagaaggt
    13681 atcgaaaaag gtatcgctaa ctccatcctg atcaaattca accagatcgg ttctctgacc
    13741 gaaactctgg ctgcaatcaa gatggcgaaa gatgctggct acactgcagt tatctctcac
    13801 cgttctggcg aaactgaaga cgctaccatc gctgacctgg ctgttggtac tgctgcaggc
    13861 cagatcaaaa ctggttctat gagccgttct gaccgtgttg ctaaatacaa ccagctgatt
    13921 cgtatcgaag aagctctggg cgaaaaagca ccgtacaacg gtcgtaaaga gatcaaaggc
    13981 caggcataag actgacttta tctgatttaa aaatgccagc ctccgggctg gcatttttta
    14041 tggttgagtt accagcggcc ggatgcgccg ccacctccag aagaaccgcc accgccgcta
    14101 aatccgccgc cacttgagcc tccaccggaa ccgctgttgt ctgaatgata acttttacct
    14161 gatgctcttt tctgaagtac acataaacag gtaaagacca taaatatggt gaaagtaaaa
    14221 aagaaggaaa atacagcaat ggaaaaaatt ttatcggaga acaggaataa atctaaaaga
    14281 tagattgctg aaatatagac tccactttta catgcgcgac aaaaattact gccttgatga
    14341 aaaaaccaga aagggaagaa catcactgca aatacccaaa aaatagcaaa gtaataatga
    14401 tcactggaag acgctgattc actttctgaa ggattagtcg gatattggtg ttgtgaagtg
    14461 agttggttat tcaaagcgtt tatagctagc tctaatccct gagcaaattt ttgttgttta
    14521 aatgcgggta tcatgttgct acggatgata tctctagcca gagcatcggt taccttttct
    14581 tccagcccat aacctacctg gatgcggaca gtacgatctg accaggcaac aatgatcagt
    14641 atcccatcat tacgtttggc atctcctaaa cgccaattgt caaaaactct tgtagcatat
    14701 tgttcaattg tctcgtcttt ggttgtggga acaattaata cagcaatttg cacttgggta
    14761 ttattgaaaa tagattctaa cttgtgcgtc agttctttct tttcttgtga gtttaagcta
    14821 ctcgtaagat ccgtaacttg ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca
    14881 aaggaacaca cgaaagtgaa cattagtatg aaatagcgca ttataaattt ctcattaaat
    14941 ctggataaat aaattgacgg cggcatcggt aattttagat tcgttatcat taaaaactaa
    15001 cacagaaaaa ataaatataa ttttggtgaa tgttttgaca tgattaaatc cattttcttt
    15061 attttcttct gacataatac gttactccct ttaattatta acgatatgtt tcgcattttt
    15121 ctttaaatct gcctgtaaca gaatgttaat aagacatgat tgttgtggca ggatctgcaa
    15181 taattaacaa ttatccactt cacagagaat gctatgcagt acccgattaa cgagatgttc
    15241 cagaccctgc aaggtgaggg ttactttacc ggcgtccccg ccatttttat tcgtttacag
    15301 ggatgcccgg ttggctgtgc ctggtgcgac accaaacaca cctgggaaaa gcttgaggat
    15361 cgggaagtct cccttttcag cattctggcg aagaccaaag agagtgataa gtggggggcg
    15421 gcgagcagtg aagatttgct ggctgtcatt ggtcggcagg gatataccgc gcggcatgtg
    15481 gtgattacgg gtggtgagcc ttgcattcat gatttgctgc cactgactga cctgctcgaa
    15541 aagaacggtt ttagctgcca gatcgaaacc agtggaactc atgaagtacg ctgcacaccg
    15601 aatacctggg ttaccgtatc gccaaagctg aacatgcgcg gcggctatga cgtgttgtca
    15661 caggcactgg agcgagccaa cgaaatcaag catccagtgg ggcgcgtacg tgatattgaa
    15721 gcactggatg aactactggc aacgctgacc gatgataaac cgcgagtcat tgcactgcag
    15781 ccgattagcc aaaaggatga tgccacacgt ttgtgcattg aaacctgcat tgcgcgtaat
    15841 tggcgtttgt cgatgcaaac acataaatat ctaaatattg cctgattaaa catttataag
    15901 cgttataaat gggtggaacc tgtaaagact tctactcatt tatattgttt gtcgcctctg
    15961 aaaactcctc cattttaccc atccagggct aatcattagc attctctaca aattctgtgg
    16021 cattaatttt tcgctggagt gaaaattatt gcggtaaagt ttggtagatt ttagtttgta
    16081 tagagttatt ttaaatattt acctttttaa tcaatggatt aagtgctctt taacataatg
    16141 gatgtgttgt ttgtgtgata ctataaagtt ggtagattgt gactggctta aaaaatcatt
    16201 aattaataat aggttatgtt tagagtgttc cccgcgccag cggggataaa ccgaaataga
    16261 cgaccagata gccgaccaga ttggagtgtt ccccgcgcca gcggggataa accgtctgtt
    16321 aatcagatta aaggtgcggt aattgagtgt tccccgcgcc agcggggata aaccggacgt
    16381 tgcgcgcact aagaaaaaca ggttcgagtg ttccccgcgc cagcggggat aaaccgctga
    16441 aatcaatcgc cggtcgtcgc cacgttacgt gttccccgcg ccagcgggga taaaccgtca
    16501 tcgaaaacca gcggatcgaa tgg
//
LOCUS       DAEVZA010000109        16426 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEVZA010000109 DAEVZA010000000
VERSION     DAEVZA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.109"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            81..260
                     /locus_tag="LAF92_003645"
     CDS             81..260
                     /locus_tag="LAF92_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792584.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            292..645
                     /locus_tag="LAF92_003646"
     CDS             292..645
                     /locus_tag="LAF92_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBI9792585.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(682..2760)
                     /gene="flhA"
                     /locus_tag="LAF92_003647"
     CDS             complement(682..2760)
                     /gene="flhA"
                     /locus_tag="LAF92_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBI9792586.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSHPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2834..3844)
                     /gene="hypE"
                     /locus_tag="LAF92_003648"
     CDS             complement(2834..3844)
                     /gene="hypE"
                     /locus_tag="LAF92_003648"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBI9792587.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLELA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3841..4962)
                     /gene="hypD"
                     /locus_tag="LAF92_003649"
     CDS             complement(3841..4962)
                     /gene="hypD"
                     /locus_tag="LAF92_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBI9792588.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4962..5234)
                     /gene="hypC"
                     /locus_tag="LAF92_003650"
     CDS             complement(4962..5234)
                     /gene="hypC"
                     /locus_tag="LAF92_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBI9792589.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(5225..6097)
                     /gene="hypB"
                     /locus_tag="LAF92_003651"
     CDS             complement(5225..6097)
                     /gene="hypB"
                     /locus_tag="LAF92_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBI9792590.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(6101..6451)
                     /gene="hypA"
                     /locus_tag="LAF92_003652"
     CDS             complement(6101..6451)
                     /gene="hypA"
                     /locus_tag="LAF92_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBI9792591.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6663..7124
                     /gene="hycA"
                     /locus_tag="LAF92_003653"
     CDS             6663..7124
                     /gene="hycA"
                     /locus_tag="LAF92_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBI9792592.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7249..7860
                     /gene="hycB"
                     /locus_tag="LAF92_003654"
     CDS             7249..7860
                     /gene="hycB"
                     /locus_tag="LAF92_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBI9792593.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7857..9683
                     /gene="hycC"
                     /locus_tag="LAF92_003655"
     CDS             7857..9683
                     /gene="hycC"
                     /locus_tag="LAF92_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBI9792594.1"
                     /translation="MSAISLINSGVAWFIAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            9686..10609
                     /gene="hycD"
                     /locus_tag="LAF92_003656"
     CDS             9686..10609
                     /gene="hycD"
                     /locus_tag="LAF92_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBI9792595.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            10627..12336
                     /gene="hycE"
                     /locus_tag="LAF92_003657"
     CDS             10627..12336
                     /gene="hycE"
                     /locus_tag="LAF92_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBI9792596.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            12346..12888
                     /gene="hycF"
                     /locus_tag="LAF92_003658"
     CDS             12346..12888
                     /gene="hycF"
                     /locus_tag="LAF92_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBI9792597.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            12888..13655
                     /gene="hycG"
                     /locus_tag="LAF92_003659"
     CDS             12888..13655
                     /gene="hycG"
                     /locus_tag="LAF92_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBI9792598.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            13652..14062
                     /gene="hycH"
                     /locus_tag="LAF92_003660"
     CDS             13652..14062
                     /gene="hycH"
                     /locus_tag="LAF92_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HBI9792599.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            14055..14525
                     /gene="hycI"
                     /locus_tag="LAF92_003661"
     CDS             14055..14525
                     /gene="hycI"
                     /locus_tag="LAF92_003661"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBI9792600.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(14684..16108)
                     /gene="ascB"
                     /locus_tag="LAF92_003662"
     CDS             complement(14684..16108)
                     /gene="ascB"
                     /locus_tag="LAF92_003662"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HBI9792601.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16117..>16426)
                     /locus_tag="LAF92_003663"
     CDS             complement(16117..>16426)
                     /locus_tag="LAF92_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBI9792602.1"
                     /translation="RLKRPLIASLISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDP
                     ANPMSIVWVFAVMALAVMLSFILTLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN
                     "
BASE COUNT         3585 a         4418 c         4546 g         3877 t
ORIGIN      
        1 ctcatggggt tatgtccggt tccctgatat taaatggggt gtgatggttt ttattttcgt
       61 tgtaatcaca taaggcgcac atgataccgg agttaatcat ttcaggggaa gaagcgaacg
      121 gtgagttctt gatgctggtt ccgggaaaga tgctgttttg cagcttttgc gtgatgctta
      181 tgaagcaagt acgcaaaatt gcgtcggttg gcgaaccttt cttctttaga gtcatagtgc
      241 cctttaagat taagatgtaa tataaataca tcttaatgag gtggaggcag catgtccggc
      301 aagcgtatct ctcgtgaaaa actgacgatt aaaaaaatga tcgatcttta tcaagcgaaa
      361 tgcccgcagg cgtcagcgga gccggagcat tacgaggcgt tgtttgttta cgcgcaaaag
      421 cggctggata aatgtgtgtt cggcgaagag aaaccagcct gtaagcagtg tccggtgcac
      481 tgttaccagc ctgcaaagcg tgaagagatg aaacagatta tgcgttgggc cgggccgaga
      541 atgctgtggc gtcatccgat cttaaccgtg cgtcatctca tcgatgacaa gcgtccggtg
      601 ccagaactgc cggaaaaata tcgtccgaaa aaacctcgtg aatagtatgc cggacaggca
      661 gatctgtccg gcaatttgca gttaaatcaa tgccgattta tcaattccca gccgcttcat
      721 ccgtgacagc agggtcgtgc gtttcagccc cagacgttgc gcagcgcctt taggcccggc
      781 aaccacgccg ttggtttctt tcagcacgcg aacaatcaac tgatattcat cttcgccctc
      841 ctgggcgaca accgttgcgg caggcggcgt ttcaggttcc ggtaaaacaa tatctggcaa
      901 tgacagctgc agcacgttac cgcgtgttag caataccgcg cgctcaatga cgttttccag
      961 ttcgcgtacg ttacccggcc actccatgtt gctcaaggtg cgcagcgtct cggcaggaat
     1021 gctgtcgata ttgcgcccca gacgacgggc aattttgaag gtaaaggctt tcgccagcag
     1081 cggaatatct tccggacgct cgcgtagtgg cggcaggtga atcgggaata cgttcaggcg
     1141 gtaatagaga tcgctacgga actctcggtc ggcgaccatt tttttcagat cgcggttagt
     1201 cgcggcgatt agacgcacgt ccgtctgaat gattttgttg ctgccgagac gttcaaactc
     1261 ctgttcctgc aatacacgca gcaacttcgg ctgtaactcc agtggcatat cgcccacttc
     1321 gtcgaggaac agggagcttt tatccgccag ttcaaaacga ccaatgcgct gggcgctggc
     1381 accggtaaaa gccccacgct catgaccaaa cagatcgctt tccagcaatc cggcaggcat
     1441 cgccgcgcag ttcattttga ccatgcggcg attattacgc ccactgagat tatggatcgc
     1501 acgggcaatc agctctttac ccgtgccagt ttcaccgagg atcagcacgg tactgtcact
     1561 ttgcgccacc atttcaactt gtttaagcac gctgtacatg gcttcgctgc ggccaataat
     1621 ctcgccaaat tcactatcaa cattgttgag ctgctcggtc agggcgaggt tttcatcaac
     1681 cagccgttct ttcagacgat ggatttcctg ataggcgagg gcgttatcga cagcgattgc
     1741 cacacgttcg gcaatctggc gcagtaaatt cagattggta gtggtaaaca ctttctcttc
     1801 gcattgcgcc agtttcagca cgcccagcat ggtgtcgcca gacatcagcg gtaacaggca
     1861 caaggtttga atctggttgc cccaggtgtc gaacaacatg cgttcatagg gggctaaatc
     1921 gtcccgctcg tggagattga tcagcagcat ctctttactt ttgaacacgc gttcggtgag
     1981 ggttccggct tcatcgactt cgctctgttc gtgggcggga tgttgtttat caagatagtg
     2041 agtggagtag atgttgagtt tgtttttacg gtggctgcgt aagacgatac tgatatcgtc
     2101 aatgtcgaaa tagtaatgga tttctttggc gacttcgctg accagttcgt ccatatccag
     2161 gcgggaaagc accgcgttgg tgatggcgac caggatgcgg aagttatcgc gttcccggca
     2221 taacaactca tagtcgacat tgttaacgac gcggctctgg atttgttcgg tgacgacaga
     2281 aacgatctgc gtaaatgttt gcagacgatt gaactctttt tcgctccagg ggcgatcgtc
     2341 ataacgaata aattcacagc caccaaaaat atgcccttcc gccgccagtg gcatcaggca
     2401 atagtgacca aattttgggt atagcccacc tgcggccagc tgcggccagg tttcacaaaa
     2461 ttcttcgtaa ctgcaatgca gcgtatcagg gtgcgacaaa atgctgcgta ccggaccgtg
     2521 tgccagaaca gtttcgtctt catatttaat gggggtgtct ttttcacgcg acgcgtaata
     2581 agacgcacgt tgagtctgcg cttgccacaa cacaatagcc gcgttgtcgg cgagcgcaga
     2641 acgctttacc agttgcgaaa gagcctcaca cagcgaggcc agatcgggct gctgcaatag
     2701 tgtccgagtg atgtcgaaca acccttgctg tccgagatca ctcatcggtg tatatgacat
     2761 tttgttcttc caggaaagca ccgcaaagcg gtgcaaaagg tcattgacta actataggag
     2821 atttagaatt ttattagcat atacgcggaa gcggttcggc gtgtggtaaa tcgagggttc
     2881 gtttcacgcc atacagaccg gcaagacgaa cacctttacg ttccaccact tcaccaatca
     2941 gcgccgcgtc tttccccagt ggatgggaat gtaacgctgc cagcacttgc tctgccgcgt
     3001 tgcgttcaac ggcgatcacc agtttgcctt cgttggcaaa gttaagggcg tccagtccca
     3061 gtagttcaca aacgccgcgc acagcaggtt taaccggcag tgccgcttct gaaatttcaa
     3121 taccacaacc gcaggctgcc gcgaactcat gaactaccgc gttcacacca ccacgggtgg
     3181 catcacgcag cgctttcacg ccgggaatgt cacgcagcgt ctgaataagc ggcgtcagta
     3241 ccgcgcagtc gctgaccagt tcgccatcca gccccagctg ctcacgcagg ttaaggatag
     3301 tcgccccgtg gtccccgagc gtgccgctaa ccagtaatac atcgcctgcg gttagcgtct
     3361 gtgcgcccca gtgaatattc gccggaattg cgcccatgcc agcggtgttg ataaacagtt
     3421 tatctgccgc gccgcgctgc accactttag tatcgccagt gacgatggca atgcctgccg
     3481 cgcgcgcggt ttctgccatg ctggtcacta cggctttcag tgtctccatc ggcaatcctt
     3541 cttcgaggat aaagccacag gagagatagc gcggaatagc gccactgacc gcaacgtcat
     3601 tggctgtgcc gcaaatcgcc agcttgccga tattaccgcc aggaaagaac agcgggtcaa
     3661 tgacgtaact gtcggtggag aacgccagac ggtcgccttc cgctaccagc tgcgccagct
     3721 caagacgtgc ctgatcttcc tgttctgcca gccacgggtt ggcaaaggct tccataaaca
     3781 ggctgttgat taattgctgc atcgcctggc cgccgctgcc gtgggcgagt tggatattat
     3841 tcacgcttca ctctcctgct gacgatactg ataccacgcg gcgcacgctc cttcggagga
     3901 gaccatcagt gcaccaaatg cggtttgagg attacaggtg ttaccaaaca gcgggcattg
     3961 atgcggctta catttgcccg ttaatacttc accacaacgt gcgcgcgggt catcgcagac
     4021 ctgctgcggt gccgggcgga aatgcgcttc ggcatcgaat cgttgataat ctggcgtcag
     4081 gtgtacacca gaagattcaa tcacgcctaa gccgcgccat tcgctgtcgc cgttgacaca
     4141 gaacacatcg gcaatcgcct gttgcgccag caggttaccg gcatccggta ccacccgacg
     4201 atactgattc tctaccttgc tgtgggccgc tattttctgc tccaccagca tgaccacgcc
     4261 ttgcagtaga tcaagcggtt cgaaaccagc caccaccagc ggacgatgaa aatcgctggc
     4321 gataaaatta taggcatcag tgccgataac catactaacg tggcccggcg cgaggaacgc
     4381 gtcgataccg ttatccggct gttccagcaa actgcgaagc gtcgggataa gcgtaatatg
     4441 ctggcagaag aagtaaaaat tctgcacatc acgcgctttc gcctgttgca gagtgatggc
     4501 ggtggtcggc atggtggttt caaaacctaa gccgaagaac accactttgc gggttggatt
     4561 ctcctgcgcc agtttcaacg catccatcgg cgagtaaacg atgcgcacat cggcaccgcg
     4621 tgcttttgcc tgcaacagcg aaccctgttt ccctggtacg cgcatggcgt cgccaaaggt
     4681 acagaagatg acttccggat ggctggcaat ctccacgcag gtgtcgattc tacccatcgg
     4741 cagtacgcac accgggcacc ccggaccgtg gataaactca acgttttcag gcagtaactg
     4801 gtcgaggccg aatttaaaaa tagcgtgggt atgaccaccg cacacttcca taatccgcag
     4861 agggcgttcg gcggtgtaag agagatgtga agcacgttcg cgcagatgct caattaactg
     4921 catcacctgt tccggcgcgc gatattcatc aacaaaacgc attatttttc ctcgccatac
     4981 aacagcgcgc cgacatccgg ctcaacgtca aacatgtttt gtaaggcgtc gagagtgtcg
     5041 cgtgcttcgg cttcattaat tacgctcatg gcaaagccaa cgtgtaccag tacccactgg
     5101 cccacgcgcg gctgaccgtt ttcatcgcag ctgccgacta acgttaaatc gacatcgcgc
     5161 tgaatgccgc agacgtcgac tttcgcctgg ttgccgtcaa tggtgcggat ctggccggga
     5221 acgcctatgc acatcgctgt gtctccagcc agttcagcca ctggtccatc ccttcgccgc
     5281 tggtggcgga aataaggatg atttcaattt ctggattgac ttcgcgggcg caggcgatgc
     5341 acttttcaac gtcaaagttg agatacggca acaggtcaac tttgttgagc agcatcagcg
     5401 aggcggcggc aaacatatgc ggatatttca gcggtttgtc ttcaccttcg gtaacggaaa
     5461 gcaccgccac tttgtgtttt tcaccgagat cgaagctggc cgggcatacg aggttgccaa
     5521 cgttttcgat aaacagaata ccgttatcgt ccagtggcag acgcggtgcg gcgtcggcaa
     5581 tcatctgtgc gtcaagatgg cagcctttac cggtgttcac ctgaatcgct ggtgtgccgg
     5641 tagcgcgaat gcgtgcggca tcgttcacgg tttgctggtc accttcaata accgcgcacg
     5701 gaacgctgtc tttcaggcgc attaaggttt ccgtcagcag ggtggtttta ccggaaccag
     5761 ggctggaaac caggttgagc accagttgct tgcgggcagc aaagcgcgcg cggttgcgtt
     5821 cagccagacg gttgtttttg tccagcacgt caatttcgac ttccagcatc cgacgctggc
     5881 tcatgcccgg tgcgtgagtg cccgcttcgc catgaccgta atgcaggtcg ccttcttcag
     5941 tctggctggg ggtaaattca ggcgctttaa tgccggtgat tttcatcttc gggcgtgccg
     6001 ccggggcaaa tggcgcgcta cgaaacgcgg aatgagggtt atgttcatca ccctcgatat
     6061 acaggttgcc ttcaccgcaa ccgcatgttg tacacatcgc tcactcctgg tctatttcta
     6121 tccgccgaat ctgtaaaccg tcgtctgcca caatctgtag catgtcacca tgacactgtg
     6181 gacagcggcg gacgcgctgg gtcagtagcg tcacatactg ttggcatgtt tcacaccagc
     6241 attcggcctc ttgttcttcg aggtgcagtt tacaaccttc cgccacgctg ccgcggcaaa
     6301 ccagatcaaa acaaaaggca agagagctgg tttcgacaca agaaaatgcg ccaattttga
     6361 gccagacccc agttacgcgt tttgcgccgt gttttgcggc ctgctgttcg atcaattcca
     6421 gtgcccgttg gcagagggtt atttcgtgca tatcgcctcc cattaactat tgccagctac
     6481 aagcaataat tgtgccagtg ttgattatcc ctgcggtgaa taatgtcgat gatgtcgaaa
     6541 tgacacgtcg acacgacgac gaaattcatc tttagcttaa aaatctcttt aataacaata
     6601 aattaaaagt tggcacaaaa aatgcttaaa gctggcatct ctgttaaacg ggtaacctga
     6661 caatgactat ttgggaaata agcgagaaag ccgattacat cgcacagcgg catcgtcgcc
     6721 tacaggacca gtggcacatc tactgcaatt cgctggttca ggggatcacg ttatcgaaag
     6781 cgcgcctgca tcacgccatg agctgcgcgc cggacaaaga actctgtttc gtcctttttg
     6841 aacattttcg tatttacgtc accctggcgg atggctttaa cagccacacc atcgagtatt
     6901 acgtcgaaac aaaagatggc gaagacaaac agcggattgc gcaggcgcaa ctgagcattg
     6961 acggcatgat tgatggcaag gtcaacatcc gcgatcgcga acaggttctg gaacactatc
     7021 tcgaaaaaat cgctggcgtt tacgacagct tatacaccgc cattgaaaac aatgtgccgg
     7081 tgaatttaag ccaactggta aagggacaaa gcccggcagc atgagctgag gctttgcccg
     7141 ttttgcaggc gttacgcctg tttggggatg ggcgtgtcga tgagtgtcga aaatgacatt
     7201 tcatcggcat gttttcgtca aaaatgacaa tcacctgagg aatgcctggt gaatcgtttt
     7261 gtaattgctg actccacgct ctgtatcggc tgccacactt gtgaggccgc ctgttcagag
     7321 acgcatcgcc agcacggcct gcaatcaatg ccgcgcctga gagtgatgct aaatgaaaaa
     7381 gaatctgcgc cgcagctctg tcaccactgt gaagatgcac cctgcgcggt ggtctgcccg
     7441 gttaacgcca tcacccgcgt tgatggggcc gtgcagttga atgaaagcct gtgcgtaagc
     7501 tgcaagctgt gcggcatcgc ctgcccgttt ggcgcaattg aattttccgg cagccgtccg
     7561 ctggatattc ctgcaaatgc caataccccg aaagcgccac cggcaccgcc tgctccggcg
     7621 cgtatcagta cgctgcttga ctgggtgccg ggtattcgcg cgatcgccgt caaatgtgac
     7681 ctttgcagtt ttgatgaaca aggtccggcc tgcgtgcgga tgtgcccgac caaagccctg
     7741 catctggtgg ataacaccga tatcgcccgc gtcagcaaac gtaagcgtga gctgactttt
     7801 aatacggact ttggcgatct caccttgttt cagcaggctc aaagtggaga ggctaaatga
     7861 gcgcaatttc cctgatcaat agcggcgtgg cgtggtttat tgccgccgct gttctggcat
     7921 ttctcttttc ttttcaaaag acgctaagtg gctggatagc cggaattggc ggcgcggttg
     7981 gtagcctgta tacggcagcc gcgggcttca ctgtgctgac tggcgcggtt ggcgtgagcg
     8041 gtgcgctgtc gctggtaagc tacgatgtgc aaatctcccc gcttaacgcg atttggctga
     8101 ttaccctcgg cctttgcggt ctgtttgtca gcctctataa cattgactgg catcgccacg
     8161 cgcaggtgaa gtgcaacggc ttgcagatca atatgttgat ggctgccgcc gtctgcgccg
     8221 tcattgccag caacctcggc atgttcgtgg taatggccga agtcatggcc ttgtgtgcgg
     8281 tgttcctcac cagcaacagc aaagagggca aactgtggtt tgcgctgggg cgtcttggca
     8341 ctctgctgct ggcgattgct tgctggctgc tgtggcagcg ttatggcacg ctggatctgc
     8401 gcctgctgga tatgcgtatg caacagctgc cgctcggttc cgatatctgg ctgctcggtg
     8461 tgattggctt tggcctgctg gccgggatta ttccgctgca cggctgggtg ccgcaggcac
     8521 atgctaacgc ctctgcgcca gctgccgcgt tgttttctac ggtagtcatg aaaattggcc
     8581 tgctgggcat tttaaccctg tcactgctgg gcggtaatgc accgctgtgg tgggggatcg
     8641 cgctgctggt gctcggcatg atcaccgcgt tcgtcggtgg tctgtatgcg ttgatggaac
     8701 acaacatcca gcgcctgctg gcttaccaca ccctggaaaa tatcggcatt atcctgctgg
     8761 ggctgggcgc tggcgtaacg ggtatcgcgc tcgaacaacc ggcgctgatt gcccttggtc
     8821 tggtcggtgg tctgtaccat ctgcttaacc atagcctgtt caaaagcgta ctgttcctcg
     8881 gggcggggag cgtctggttc cgtaccggtc atcgcgatat cgaaaaactc ggtggtattg
     8941 gcaagaaaat gccggttatc tccatcgcca tgttagtcgg gttgatggca atggctgcgc
     9001 tgccgccgct gaacgggttt gccggggaat gggttatcta tcaatccttt ttcaaactga
     9061 gcaatagtgg cgcgtttgtt gcccgtctgc tggggccgct gctcgctgtg ggtctggcaa
     9121 ttaccggtgc gctggcggtg atgtgtatgg cgaaagtcta tggcgtcacg ttcctcggcg
     9181 cgccgcgtac caaagaagcc gaaaacgcca cctgtgcgcc gctcctgatg agcgtaagcg
     9241 tagtggcact ggcgatttgc tgcgtaattg gcggtgttgc tgcgccgtgg ctgctgccga
     9301 tgctctctgc tgctgtacct ctgccgctgg agcctgctaa caccaccgtt tctcaaccga
     9361 tgatcacatt gctgctgatt gcctgcccgc tgctgccatt catcattatg gcgatttgca
     9421 aaggcgatcg tttgccgtcg cgttcccgcg gtgcggcctg ggtgtgcggc tacgaccacg
     9481 aaaaatcaat ggtgattacc gctcacggtt ttgccatgcc ggtgaaacag gcgtttgcgc
     9541 cggtgctgaa actacgcaaa tggctgaatc cggtgtctct ggtgccgggc tggcagtgcg
     9601 aggggagtgc gttgctgttc cgccggatgg cgctggttga actggcggtg ctggtggtga
     9661 ttattgtttc acgaggagcc tgagaatgag tgttttatat ccgttaattc aggcgctggt
     9721 gctatttgcc gttgcgccgc tgctctccgg tattacccgc gtggcgcgag cccgcttaca
     9781 taaccgtcgt gggccgggtg tgttgcagga gtatcgtgac attatcaaac tgctgggccg
     9841 ccagagcgtt ggcccggatg cctccggctg ggtgttccgc ctgacgccgt atgtgatggt
     9901 gggcgtcatg ctgactatcg ctactgcgct gccggtggtg accgtcggtt ctccgctgcc
     9961 gcaactgggt gatttaatca ccttactgta tctctttgcc atcgcgcgtt tcttctttgc
    10021 catttctggt ctggataccg gtagcccgtt taccgctatc ggcgcgagcc gtgaagcgat
    10081 gcttggcgtg ctggtcgaac cgatgctgct gcttggtctg tgggttgccg cacaggttgc
    10141 cggttccacc aacatcagca acatcaccga caccgtttat cactggccgc tgagccagag
    10201 catcccgctg gtactggcgc tttgtgcctg tgcgttcgct acctttatcg aaatgggcaa
    10261 actgccgttc gacctggcgg aagccgagca ggagttgcag gaaggtccgc tctccgaata
    10321 cagcggtagc ggctttggcg tcatgaaatg gggtatcagc ctgaaacagc tggtggtgtt
    10381 gcagatgttc gtcggggtgt ttattccgtg gggacaaatg gaaaccttca ccgccggtgg
    10441 actgctgctg gcgctggtga ttgccatcgt caaactggtg gtcggcgtcc tggttatcgc
    10501 gctgttcgaa aacagcatgg cccgtctgcg tcttgatatt actccgcgca ttacctgggc
    10561 tgggtttggc tttgcatttt tagcgttcgt ctccttgctg gcggcgtgat taaagagagt
    10621 ttgagcatgt ctgaagaaaa attaggtcaa cattatctcg ccgcgctgaa tgaggcattt
    10681 ccgggcgtcg tgctggacca cgcctggcag accaaagatc agctgactgt caccgtaaag
    10741 gtgaactacc tgccggaagt ggtggagttt ctttactaca aacagggtgg ctggctgtcg
    10801 gtgctgtttg gtaacgacga acgcaaactg aatggtcatt acgccgttta ttacgtgctg
    10861 tcgatggaga agggcactaa gtgttgggta acggttcgcg tcgaagttga cgccaacaaa
    10921 ccggagtatc cgtccgtgac gccgcgcgtt ccggcggctg tgtggggcga gcgcgaagtg
    10981 cgcgatatgt acggtttgat tccggttggt ctgccggatg aacgtcgtct ggtgctgccg
    11041 gatgactggc cggatgaact ttatccgctg cgtaaagaca gcatggatta tcgtcagcgt
    11101 ccggcgccga ccaccgatgc tgaaacctac gagttcatca atgaactggg tgacaagaaa
    11161 aacaacgtcg tgccgattgg tccgttgcac gtcacttccg acgaaccggg tcacttccgt
    11221 ctgttcgtcg atggcgaaaa tattatcgac gccgactacc gcctgttcta cgtccatcgc
    11281 ggcatggaaa aactggcgga aacccgtatg gggtacaacg aagtgacctt cctctctgac
    11341 cgtgtgtgcg gcatctgcgg ctttgctcac agcaccgcct acaccacgtc ggtggaaaac
    11401 gcgatgggta ttcaggtgcc agaacgtgcg cagatgatcc gcgccattct gctggaggtg
    11461 gaacgcctgc actcgcatct gctcaacctc ggcctggcct gtcactttac tggcttcgac
    11521 tccggcttta tgcagttctt ccgcgtgcgt gaaacctcca tgaaaatggc agagatcctt
    11581 accggtgcgc gtaaaaccta cggcctgaac ctgatcggcg ggattcgtcg cgatctgctg
    11641 aaagatgaca tgatccagac ccgccagctg gcgcaacaaa tgcgtcgtga agtgcaggag
    11701 ctggtggatg tgctgctgag tacgccgaac atggaacagc gcactgtcgg cattggtcgt
    11761 ctggacccgg aaatcgcccg cgatttcagt aacgttggcc cgatggtccg cgccagcgga
    11821 cacgctcgcg atacccgcgc cgatcacccg tttgttggtt atggcctgct gccaatggaa
    11881 gtccacagcg agcagggctg cgacgttatt tcccgtctga aagtgcgtat caacgaagtc
    11941 tataccgcgc tgaacatgat cgactacggt ctggataacc tgccgggtgg tccgctgatg
    12001 gtggaaggct ttacctacat tccgcaccgt ttcgcgctgg gctttgccga agcgccgcgc
    12061 ggcgacgata tccactggag catgaccggc gacaaccaga agctgtaccg ctggcgctgc
    12121 cgtgccgcga cctacgcgaa ctggccgacc ctgcgctaca tgctgcgtgg caataccgtt
    12181 tccgatgcgc cgctgattat cggtagcctg gacccttgct actcctgtac cgaccgcatg
    12241 accgtggtcg atgtgcgtaa gaagaagagc aaagtggtgc cgtacaaaga actcgagcgt
    12301 tacagcattg agcgtaaaaa ctcgccgctg aaataaggaa tcgccatgtt tacctttatc
    12361 aaaaaagtca tcaaaaccgg cacggcgacc tcgtcttatc cgctggagcc gattgcggtt
    12421 gataaaaact tccgtggtaa gccagagcag aacccgcagc agtgcatcgg ctgcgcggcc
    12481 tgcgtcaatg cctgcccgtc aaacgcctta acggttgaaa ctgacctcgc cacaggagag
    12541 cttgcgtggg agtttaatct cgggcgctgc atcttctgtg gacgctgcga agaagtctgc
    12601 ccgacggcag cgatcaaact gtcgcaagag tacgaactgg cggtgtggaa gaaagaagat
    12661 ttcctgcaac agtcccgttt tgtgctgtgc agctgtcgcg tctgcaatcg tcctttcgcc
    12721 gtccagaaag agatcgacta cgccattgcg ctgcttaagc acaatggtga cagccgcgcg
    12781 gaaaaccacc gcgaaagctt tgagacttgc ccggaatgta agcgccagaa atgcctggtg
    12841 ccgtccgacc gtattgaact gactcgccat atgaaagagg ccatctgatg agcaatttat
    12901 taggcccccg tgacgccaac ggcattccgg tcccgatgac ggtggatgaa tccatcgcca
    12961 gcatgaaggc gtcgttactg aaaaaaatca aacgttctgc ctacgtttac cgcgtggact
    13021 gcggcggctg caacggctgc gaaatcgaaa ttttcgccac gctttcgccg ctgtttgatg
    13081 cagaacgctt cggcattaaa gtcgttcctt caccgcgtca tgcggatatt ttactgttta
    13141 ccggcgcggt cacccgtgca atgcgatccc ctgcgctgcg tgcgtggcag tccgcgccgg
    13201 acccgaaaat ctgtatctcc tacggtgcct gcggtaacag tggcgggatc ttccacgatc
    13261 tctactgcgt gtggggcggt acggataaaa tcgtcccggt agatgtctac atccctggtt
    13321 gcccgccaac gcctgccgcc acgctgtacg gctttgcaat ggcgctcggc ctgctggagc
    13381 agaaaattca cgcccgtggc ccgggtgagc aggatgaaca accggcggaa atcctgcatg
    13441 gcgatatggt gcagccgctg cgtgtgaaag tggatcgcga agcacgccgc ctggcaggtt
    13501 atcgttacgg tcgtcagatt gccgatgact tcctgacaca gttagggcag ggcgaagaac
    13561 aggttgcacg ctggctggaa gcggaaaacg atccgcgtct gaacgagatt gtcagccatc
    13621 tgaatcatgt tgtagaagag gcgcgtatcc gatgagtgaa aaggtggtgt tcagtcaact
    13681 gagccgtaaa tttattgatg agaacgatgc cacgcccgcc gaggcgcagc aggtggtcta
    13741 ttacagcctg gcgattggtc accaccttgg ggttatcgat tgcctggaag cggcgctcac
    13801 ctgcccgtgg gatgaatatc tggcatggat tgccactctg gaggcaggca gtgaagcccg
    13861 ccgcaaaatg gaaggcgtgc cgaaatatgg tgagatcgtc atcgacatta accatgtgcc
    13921 gatgctggcc aacgcattcg ataaagcccg ggcagcgcaa acttcgcagc agcaggaatg
    13981 gagtacaatg ctgttaagta tgctgcatga tattcatcag gaaaacgcca tctatttgat
    14041 ggtgaggaga ctgcgtgact gacgttttac tctgtgttgg caatagcatg atgggcgatg
    14101 atggcgcagg tccgctgctg gcggaaaagt gcgccgccgc gccgaaaggt aactgggtgg
    14161 tgattgacgg cggtagcgca ccggaaaacg acatcgtcgc tatccgtgaa ctgcgcccga
    14221 cacgactgct gattgtcgac gccacggata tggggctaaa ccccggcgag atccgcatca
    14281 tcgacccgga tgatatcgcc gagatgttta tgatgactac ccataacatg ccgttgaatt
    14341 accttatcga ccagttgaaa gaagatattg gcgaagtgat tttcctcggc attcagccgg
    14401 atatcgtcgg cttttactac ccgatgaccc agccgattaa agatgcggta gaaaccgttt
    14461 atcaacgact ggaaggctgg gaaggaaatg gcggcttcgc gcagttagcg gtggaagaag
    14521 agtagttttt cattaaggaa tcaggacagg gatgttcttg atggggtgaa ccagctctga
    14581 tgccaaatgc taaattgccc gatgcgctgc gcttatcggg ccttcatggt tcgtgcgaca
    14641 tgtaggccgg ataaggcgtt cacgccgcat ccggcacggt tacttactct aaatcttccc
    14701 cattactggc aattactttt ttataccacc agaacgattt cttacgcgtg cgcgtcagcg
    14761 tgccgttgcc tgcgtcgtca cggtctacgt agacaaaacc gtagcgtttg ctcatttcac
    14821 ccgtagaggc ggaaactaaa tcaatacagc cccaggtggt gtagcccatc agcggaatgc
    14881 catctgcaat cgcttcgccc attgcgcgga tatgttcgcg taaatagctg atgcgatagt
    14941 catcgttaat ctcgccattg gcagcaagtt catcttttgc gccgaggccg ttttccacca
    15001 gaaacagcgg cttctgataa cgatcgtaca tcatattcat ggtgatacgc agaccgagtg
    15061 gatcaattcc ccagccccag tcgctcacct gtagatacgg attacgcagc gatttcacca
    15121 cgttcgccgc actgctgttg ttggcgttca tctccgccga ggcgcagcgc gaggcgtaat
    15181 agctgaaaga gacaaaatcg acggtgtttt tcaggatttc atcatcgccc ggtgctttgt
    15241 tgatggttac ccctttttcg cgaaatacgc gggcagagta agccggatac gcgccgcgcg
    15301 cctgcacatc gataaaaaac aggttttccc gatctttctc cagcgccgcc cagacatctt
    15361 ccggtttgca gctgtaaggg tagaagttac cgcccgccag catacagccc acctgatttt
    15421 gcgggttaac ctcatgggcg attttggtgg ctagcgcact ggcaaccagc tggtgatgcg
    15481 cggcctgata tttcacctga tcctgatttt caccttcttc aaacaccaga cccgcgccgg
    15541 agaacgggct atgcaacata atgttgattt cattgaaggt aagccagtat ttcaccagac
    15601 catcaaatgc ttcaaagcag gtccgggcgt agcggctgaa aaactccacc agcttgcggt
    15661 tacgccagga gccatattcg gtgaccagat gcatcggcac atcgaagtgg cacaacgtga
    15721 ccagcggttc gataccgtac tttttacact cttcaaagac agaacgataa aaagcaatgc
    15781 cctgctgatt gggcgtgatt tcatcgccct gcggaaagag acggctccag gcaattgagg
    15841 tacggaaaac cttgaatccc atctctgcca tcagggcgat atcttcttta taacgatgat
    15901 aaaaatccgt cgcctcatgg ctgggataaa actcgtcatc gcgcaactga aaacgttttt
    15961 ccagccccag tttcaccgcc attcgatgct cgccgtgtgg gatcatatcg acagtggtca
    16021 gccctttgcc accttcacgg aacgcacctt cagactggtt ggcggcaagc gcgccgcccc
    16081 ataaaaaacc ttctggaaat actgacattt tcatcctcaa ttaagactta cttctttggc
    16141 gacggtcggt tgtacgctct gatgctttcg cgcctcggca gtcgcttcct caacaggaat
    16201 atcctcaaag ccgagcaaca gtgtgaggat aaacgacagc atgaccgcca gcgccatgac
    16261 cgcgaacacc cagacgatgc tcattggatt cgccggatcg aagaactgca cgctggtaaa
    16321 tagccccggc gctgccattg agtggctggc aagccccgcc ataccggcaa ccgcgccgca
    16381 aataaaaccg ctgataagac tggcgataag cggacgtttc aggcgg
//
LOCUS       DAEVZA010000110        16279 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEVZA010000110 DAEVZA010000000
VERSION     DAEVZA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.110"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..633
                     /gene="lplA"
                     /locus_tag="LAF92_003664"
     CDS             <1..633
                     /gene="lplA"
                     /locus_tag="LAF92_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBI9792603.1"
                     /translation="AEGDRKVSGSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKK
                     LAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEAEIISPDKTPDLP
                     NFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHITRAQVFTDSLNP
                     APLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIAGAVR"
     gene            665..814
                     /locus_tag="LAF92_003665"
     CDS             665..814
                     /locus_tag="LAF92_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792604.1"
                     /translation="MIDLENQEREIINIMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(1089..1808)
                     /gene="deoD"
                     /locus_tag="LAF92_003666"
     CDS             complement(1089..1808)
                     /gene="deoD"
                     /locus_tag="LAF92_003666"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBI9792605.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(1888..3111)
                     /gene="deoB"
                     /locus_tag="LAF92_003667"
     CDS             complement(1888..3111)
                     /gene="deoB"
                     /locus_tag="LAF92_003667"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBI9792606.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(3163..4485)
                     /gene="deoA"
                     /locus_tag="LAF92_003668"
     CDS             complement(3163..4485)
                     /gene="deoA"
                     /locus_tag="LAF92_003668"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBI9792607.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(4612..5391)
                     /gene="deoC"
                     /locus_tag="LAF92_003669"
     CDS             complement(4612..5391)
                     /gene="deoC"
                     /locus_tag="LAF92_003669"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBI9792608.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            5649..7199
                     /locus_tag="LAF92_003670"
     CDS             5649..7199
                     /locus_tag="LAF92_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBI9792609.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            7171..8034
                     /locus_tag="LAF92_003671"
     CDS             7171..8034
                     /locus_tag="LAF92_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBI9792610.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8147..8929)
                     /locus_tag="LAF92_003672"
     CDS             complement(8147..8929)
                     /locus_tag="LAF92_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI9792611.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFAVLCELRSEPADEIAEVLLNNTYAVFSVSG"
     gene            complement(8926..9999)
                     /locus_tag="LAF92_003673"
     CDS             complement(8926..9999)
                     /locus_tag="LAF92_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBI9792612.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(10121..10282)
                     /locus_tag="LAF92_003674"
     CDS             complement(10121..10282)
                     /locus_tag="LAF92_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBI9792613.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(10409..11014)
                     /gene="osmY"
                     /locus_tag="LAF92_003675"
     CDS             complement(10409..11014)
                     /gene="osmY"
                     /locus_tag="LAF92_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBI9792614.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(11407..12996)
                     /gene="prfC"
                     /locus_tag="LAF92_003676"
     CDS             complement(11407..12996)
                     /gene="prfC"
                     /locus_tag="LAF92_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBI9792615.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(13087..13764)
                     /gene="yjjG"
                     /locus_tag="LAF92_003677"
     CDS             complement(13087..13764)
                     /gene="yjjG"
                     /locus_tag="LAF92_003677"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBI9792616.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(13779..14225)
                     /gene="rimI"
                     /locus_tag="LAF92_003678"
     CDS             complement(13779..14225)
                     /gene="rimI"
                     /locus_tag="LAF92_003678"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBI9792617.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(14194..14607)
                     /gene="holD"
                     /locus_tag="LAF92_003679"
     CDS             complement(14194..14607)
                     /gene="holD"
                     /locus_tag="LAF92_003679"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBI9792618.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            14710..15741
                     /gene="rsmC"
                     /locus_tag="LAF92_003680"
     CDS             14710..15741
                     /gene="rsmC"
                     /locus_tag="LAF92_003680"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBI9792619.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            16042..16128
                     /locus_tag="LAF92_003681"
     tRNA            16042..16128
                     /locus_tag="LAF92_003681"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16076..16078,aa:Leu,seq:cag)
     gene            16157..16243
                     /locus_tag="LAF92_003682"
     tRNA            16157..16243
                     /locus_tag="LAF92_003682"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16191..16193,aa:Leu,seq:cag)
BASE COUNT         3799 a         4393 c         4256 g         3831 t
ORIGIN      
        1 gccgaaggtg accgcaaagt ctcaggatcg gcctatcgcg aaaccaaaga tcgtggcttc
       61 caccacggca ccttgctgct caatgccgac cttagccgcc tggcaaacta tctcaatccg
      121 gataaaaaga aactggcggc gaaaggcatt acctcagtgc gttcccgcgt gaccaacctc
      181 accgagctgc tgccggggat cacccatgag caggtttgcg aggccataac caaggccttt
      241 ttcgcccatt atggcgagcg tgtagaagcg gaaatcatct ccccggacaa aacgccagac
      301 ttgccaaact tcgccgaaac ctttgcccgt cagagtagct gggaatggaa cttcggtcag
      361 gctcctgcat tctcgcatct gctggatgaa cgctttagct ggggcggcgt ggaactgcat
      421 ttcgacgttg aaaaaggcca tatcacccgc gcccaggtgt ttaccgacag cctcaacccc
      481 gcgccgctgg aagccctcgc cgggcgactg caaggcggcc tgtaccgcgc ggatatgctg
      541 caacaagagt gcgaagcgct gttggttgac ttcccggacc aggaaaaaga gctacgggag
      601 ttgtcgacgt ggatagcggg ggcggtaagg taactcgctg attaaaatac caaaggaagg
      661 gaatatgatc gatctggaaa accaggagcg ggagattatc aatataatgt taagccagcg
      721 tatttcctgg ctagccgccg tgcgtatcag gcataaatta tcgcttgctg aagtaagtaa
      781 aatgttaggt atctcgatta attctctaaa atagatcgaa aagacggaac ggtttagctc
      841 aaacatcaaa agcaaaatgg cagaaattta tggttgtcca ccagagttgc tgatatgccc
      901 ttcctggatg acggctgaac ataaatgaat agattcgtga atagatactt gaatagattt
      961 tattttctat ccatatgaaa aataatagta aatctataaa tcagttgcta ttgatgaata
     1021 gatactaaaa agccggagca gtctcccact ccggcttcac acgacaatcg cctttcagcg
     1081 aaacacaatt actctttatc gcccagcaga acggattcca gtgcgatttt gatcatgtcg
     1141 ttgaaggtgg tctgacgctc agcggcagtg gtctgctcgt gagtgcggat gtggtcagac
     1201 acggtacaga tggtcagggc tttcgcgcca aattctgcag cgacgccgta gataccagcc
     1261 gcttccattt ccacgccgag gatgccgtat ttttccatca cgtcgaacat ttcgccgtcc
     1321 ggagagtaga acaggtcagc ggagaacagg ttaccaacgc gagcatcaat gcccagtgct
     1381 ttagccgcgt ctaccgcgtt acgcaccatg tcaaagtcag cgatagcggc aaagtcatga
     1441 tctttaaaac ggatgcggtt aactttggaa tcggtgcagg cacccatacc gataacgacg
     1501 tcgcgcagtt ttacgtgcgg cagaactgcg ccacaggaac ccacgcggat aattttcttc
     1561 acgccgaaat cggtgatcag ttctttggtg tagatggagc aggacgggat acccataccg
     1621 tgacccatta cggaaatttt gcggccttta taagtaccgg tgaagcccag cataccgcga
     1681 acgttgttca cttcacgggc atcttcaagg aaagtttcag caatatactt tgcacgcagc
     1741 gggtcgcctg gcatcaaaac tacgtcagcg aaatcgccca tttctgcatt aatgtgtggg
     1801 gtagccattg ttttatcctt atctacatgt tttcaccctc tccctgaggg agagggccgg
     1861 ggtgagggca tcaagccgca cccattatca gaacatggct ttgccatatt ccatatcaga
     1921 agtaccaaaa tattttgcca gagtctggcc gatatccgcg aaggtttcac ggtgacccag
     1981 tgagcccggt tttactttcg ggccgtaaac cagtaccgga atgtgttcac gcgtgtgatc
     2041 agtaccggtc caggtcggat cacaaccgtg gtcagcagtg aggatcagga tgtcgtcatc
     2101 gcgcagcaga gacatcagct ccggcagacg gcggtcgaac agttccagac ccgccgcata
     2161 acctgcgacg tcgcgacggt ggccccagga agagtcgaag tcaacgaagt tggtgaagac
     2221 gatggtgtta tcacccgctt ctttcatctc tttgatggtg gcgtcaaaca gcgcgtccag
     2281 gccagttgct ttcactttct tggtgatacc gcagttggcg tagatgtctg caattttacc
     2341 gacagaaacc acctgaccgt gtttttcatc aaccagtttc tgcagcacgg tcggtgctgg
     2401 cggctcaaca gccaggtcgt gacggttacc ggtacgctgg aagttaccgg ctttgtcgcc
     2461 aataaacgga cgagcgataa cacgaccgat gttgtagccg ccgttggtca gctcttcacg
     2521 ggcgatttcg cacagttcgt agagtttatc caggccaaag gtttcttcgt ggcaggcaat
     2581 ctggaacacg gagtcagcgg aggtatagaa aatcggcttg ccggttttca tgtgctcttc
     2641 gcccagctga tccagaatga ccgtaccgga agagtggcag ttaccgagat aaccaggcag
     2701 gttagcgcgt tcaaccagtt tatccagcag ctcttgcggg aagctgtttt cgtgatcgga
     2761 gaagtatccc cactcaaaca gaaccgggac accggcgatt tcccagtgac cagacggggt
     2821 atctttaccg gatgacattt cgtgcgccca tgcgtacgcg ccgataactt cagcgttgcc
     2881 gtccattccc gccggaatga aaccggtaga accttcgtgt gctttcgcca gccccagacg
     2941 ggtcagattt ggcagattga gcgggccttt acgaccgtta tcagcttcgc ctttagcaca
     3001 agcttctgcg atatgaccca gggtgtcagc cccgacgtca ccaaagcgtt ctgcatcttc
     3061 tgtagcgccg atgccgaatg agtccagcac cataataaat gcacgtttca tatgttctcc
     3121 gtactcagtg cttcaaattt aaatgatcag atcagtatac cgttattcgc tgatacggcg
     3181 atagacagtt ggtgtgcttt ccggtgcttt atcggcaagt ttaattgccg ctttcaccgc
     3241 tttcgccgct tcctgccagc tgttttcgtc tttcgcgtgg ataaccgcca gcggacgctg
     3301 accgtctacc tggtcgccca gacgcgccat atcagtaaag ccgacgctgt aatcaatggt
     3361 gtcagatgcc tgacgacgac cgccgcccat tgcaaccact gccatcccca gcgcgcgggt
     3421 atccatttca ctgacaaaac cttcggtatc agcatagact gctttcgtca gcatcgctgt
     3481 cggcagatac ttcgcgtagt tctcaacgaa gtcggttggg cctttttgtg ccgctaccat
     3541 acgaccaaag acttctgccg ctttaccgtt gtccagcact gcttgcaatt tcgcgcgcgc
     3601 ttcggcgtca tctttcgcca gtttgccgga gataagcatc tccacgcaca gcgccatcgt
     3661 gacatcaaac agacgcgggt tacgatattc acccgtcagg aactgcaccg cttcacgaac
     3721 ttcaaccgcg ttacctgcac tggaggccag tacctgattc atgtcggtga gcagcgcggt
     3781 ggtgcgcacg ccagcgccgt tagccacgcc aacaatcgct tcggcaaggg cttcagagag
     3841 ttcgtaggtc ggcataaacg cgccgctacc cactttcacg tccatcacca gcgcatccag
     3901 accttccgcc agtttcttcg ccaggatcga ggcagtgatc agcgggatgg agtccacggt
     3961 tgcggtaata tcacgggtcg cgtagaaacg tttatccgcc ggagccagtg agctggtctg
     4021 gccgataatc gctacgccga cgtctttaat aatttcgcgg aaacggttgt catccgggaa
     4081 aatgtcgaag ccagggatgg attccagttt gtcgagcgta ccgccagtat gaccgaggcc
     4141 gcgaccggag atcatcggaa tatagccgcc gcaggctgcg accatcggcc ccaacatcag
     4201 cgaagtcaca tcgccgacgc cgccggtgga gtgtttatca acaatcgggc cattcagatg
     4261 caggcttttc cagtcgagaa cggttcctga atctcgcatc gccatggtca gcgagacacg
     4321 ctcaggcatt gtcatatcgt ggaagaaaat ggtcatcgcg agggcggcaa tctgcccttc
     4381 ggagatagtg ttgtcgcgaa taccgttgat aaagaaacga atttcttcat cgcttagcgc
     4441 atgaccatca cgttttttac gaataatttc ttgtgcgaga aacaaggtaa cctcccgagg
     4501 aaaagagtat gaggcggctt ttgccggatg cgtctacgcc ttatccggcc tacgggtaga
     4561 tacgtctcgt aggcctgata agcgcagcgc atcaggcgta aagcatctta cttagtagct
     4621 gctggcgctc ttaccgtcac cgtggcccag cgctttcaac aggcttgcca gcaggctgga
     4681 agcaccaaag cggtagtgac gcgcatctgc ccagtcagca ccgaacagtt catctgcgat
     4741 ggcgagatat ttctgcgcat cttccgcagt acgcacgccg cccgctggtt tgaaaccaac
     4801 ggttttttct acgcccatat cacggatcac ttccatcatg atgcgcgcgc tttccggcgt
     4861 cgcgttcaca gccactttac cggtagaggt tttgatgaag tccgcacccg ctttgatgga
     4921 gatttcagac gctttacgga tcagcgcttc gtctttcagt tcgccggttt cgatgatcac
     4981 tttcagcagt acattcgctg ccgcgcaagc ctctttacag gctttcacca ggtcaaaacc
     5041 aacctgctcg ttacccgcca tcagcgcgcg gtacgggaac acaacgtcaa cttcatcagc
     5101 accgtaggcg attgccgcac gggtttctgc cagcgcgatt tcgatgtcat cgttaccgtg
     5161 tgggaagttg gttaccgtag cgattcggat ttccggggtg ccctgctctt tcagcgtttt
     5221 gcgagcaatc gggataaagc gaggatagat acagatagcg gcggtattgc cgaccggagt
     5281 tttggcctga tgacacagag caattacttt ctcgtcggtg tcgtcgtcat tcagggtggt
     5341 caggtccatc aatttcagtg cacgcaggct gcttgctttc agatcagtca tttcattctc
     5401 ctggcttgtc gccaataaaa ttcaccttgc gagtttgtta gtattctaac atctactccg
     5461 caacacactt cgatatacat cacaattaag gaaatctact tacaagtttg catcactgta
     5521 atgcgatctg gttcaaataa ttcactttca aatgaatgcg tcagtggtgg caaacgcatc
     5581 aggatcaaag tgaacatcac gaaacttctt acaatggcgc atcaccttcg gcataaaagg
     5641 aaaagaagat gcccacttct catgaaaatg cactgcaaca acgttgccag caaattgtca
     5701 ccagcccagt gcttagcccg gagcagaagc gccattttct ggctctggaa gcagaaaaca
     5761 atctgcctta tccgcagctt cctgccgaag cccgccgcgc gctggatgag ggtgtaatct
     5821 gcgatatgtt tgaaggtcat gcgccgtaca aaccgcgcta tgtcttaccc gattacgccc
     5881 gttttctggc gaacggttcc gaatggctgg agctggaagg cgcgaaagat cttgatgacg
     5941 cactctctct gctgaccatt ctttaccacc acgtaccgtc ggtcacatcg atgccggtct
     6001 acctggggca actggatgcg ttgttgcaac cgtatgttag aattctaaca caagacgaga
     6061 tcgatgttcg aataaaacgt ttctggcgtt acctcgacag aaccctgcca gacgccttta
     6121 tgcacgccaa tatcggcccg tctgattcgc ccattacccg cgcgatctta cgtgcagacg
     6181 cagagttgaa gcaggtttca ccgaacctga cctttatcta cgatcctgaa atcacccctg
     6241 atgacctgct gctggaagtg gcgaagaaca tctgtgaatg tagcaaaccg cacatcgcca
     6301 acggtccggt gcatgataaa attttcacaa aagggggcta cgggattgtg agctgttaca
     6361 actcactgcc gctggcgggt ggtggcagca cgctggtacg ccttaacctg aaagccattg
     6421 ccgagcgcag cgaatcgctg gatgacttct ttacgcgcac tctaccgcac tactgccagc
     6481 agcagatcgc catcatcgat gcgcggtgtg aattcctcta tcaacaatca cacttctttg
     6541 agaatagctt cctggtgaaa gaagggctga ttaaccctga acgttttgtg ccaatgtttg
     6601 gcatgtatgg gctggcggaa gcggttaact tgctgtgtaa gaaagaaggg attgccgcgc
     6661 gctacggtaa agaagccgcc gcaaatgaag tgggttatcg catcagcgcg caactggcgg
     6721 agtttgtcgc caataccccc gtgaaatatg gctggcaaaa acgcgccatg ttacacgcac
     6781 agtcggggat cagttccgat atcggcacca cgccgggcgc gcgtttgccg tatggcgatg
     6841 agccagatcc gatcacccat ctgcaaactg tcgccccgca tcatgcttat tattattccg
     6901 gcatcagcga cattctgacg ctcgacgaaa ccatcaaacg taacccgcag gcactggtac
     6961 agctttgcct cggtgccttt aaagccggaa tgcgtgaatt taccgccaat gtcagcggta
     7021 acgatctggt tcgcgttacc ggttatatgg tgcgtttgtc ggacttagaa aaatatcgcg
     7081 ccgaaggttc acgcaccaac accacctggc tgggcgaaga agccgcacgc aacactcgta
     7141 ttctggaacg ccagccgcgc gtgataagcc atgaacagca gatgcgcttt agtcagtaag
     7201 attatcccct tctcctgcgt tgacgggcca ggcagtcgtc tggctctgtt tttgcagggc
     7261 tgcaatctgc gctgcaaaaa ctgtcacaat ccgtggacga tgggacgttg caatgactgt
     7321 ggggagtgcg tgccacagtg tccgcatcag gcgttgcaga ttgttgacgg caaagtggtg
     7381 tggaacgctg tggtttgcga gcagtgtgat acctgcctga agaggtgtcc gcaacatgcc
     7441 acgccaatgg cgcaatccat gagcgtggac gaagtgctta gccatgtccg caaagcggtg
     7501 ctgtttattg aagggataac ggtaagcggc ggcgaagcca cgacccaact gccgtttgtg
     7561 gtggcgctgt ttacagccat caaaaacgat ccgcaactgc gccacctcac ctgcctcgtg
     7621 gacagtaacg gtatgttgag cgaaaccggc tgggaaaaat tactcccggt gtgcgacggc
     7681 gcgatgctcg atctcaaagc gtgggggagc gaatgtcatc agcaattaac cggacgcgat
     7741 aatcagcaga ttaagcgcag catctgtttg ctggcagagc gcggtaagct ggcggaactg
     7801 cgtttgctgg taattccaga ccaggtggat tatttgcatc acatcgatga gctggcgacg
     7861 tttatcaaga gacttggcga tgttccggtt cgcctgaatg cgtttcatgc ccacggcgtg
     7921 tatggcgagg cgcaaagctg ggcgagcgcc acgccggaag acgttgagcc gttggctgat
     7981 gcgttaaagg tgcgcggggt gagccggttg atatttccgg cgctctattt gtgacatgcc
     8041 ggatacggcc tacggttcgg cacaaacctg taggcatgat aagacgcgac aagcgtcgca
     8101 tcaggcatta tgcgccgact gccggatgct gcctacgaag ggcaaactac ccactaacgc
     8161 taaacaccgc atacgtatta ttaagcaaca cttcggcaat ctcatccgcc ggttccgagc
     8221 gcaactcgca cagcacggca aatacccggg cggcctgttc cgggcggtta gcctgcccct
     8281 gaaaaccatt gagcggcata tccggcgcgt cggtttccag caacaacgat gccagcggta
     8341 attttgcgat gacatcgcgg gttttactgg cgcgtggata ggtgatagta ccgcctacgc
     8401 cgattttgta gcccagctgt acaaaccgtt cggcctgttg caggctgccg gaaaaaccgt
     8461 gaaccacgcc agtgcgcgat aaatcgtggc gtttaagatg catcgccagt ttgtcgtgtg
     8521 tgcgccggga atgaaggatc accggcagat cgtagcgttt cgccagtttc agctgttcgt
     8581 cgagtaacca ctgctgcctc tcaaattgcg gatcgtcgcc gaagagatcc agaccgatct
     8641 cccccaccgc caccaccttc gccggacgcc tttccagcgc ctgctgtagc tgatcaagag
     8701 acacatcgct atgtttttcc aacatccccg gatgcaaccc caatgcggca taaagcggtt
     8761 gatatttttc cgctaacgcc tgcacgcggg caaaattttc cgcctctgtc gccggaacaa
     8821 tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct ggcctcttca tcgccactaa
     8881 acggcgggaa atcaaaatgg cagtgggtat cgataaaacg gcaaatcaag ccagatcctc
     8941 attgttaaat gtcgggtcgt tcgcctgcgg agcatcgctc acttcggcaa ccagcgtatc
     9001 gttggcgact ggcgcaggcg gaataacgat cgattccggc gtcaccactg gcaccagatg
     9061 gcgggtaagc ggcgcttttt cagttaatag cttgccaacc gtggcgagga aataacgacc
     9121 gcataaacgc ccaagtttat agtcttcacg cagcgccgga atccgactgc caagcgcgat
     9181 actatgtaat ggcttcggtg gataaatttc gaatatccgc agcttgcccg gtggtttctc
     9241 aataaattgc tgaatgtcac gatagctggt ttcatgatgc tgcaccagat tgaccagcgg
     9301 ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg aaccactgcg gcgtgtagta
     9361 catttgtgac ggcacagtgc ggatgacgac caacgtttta gcgccctgcc ttgccgcctc
     9421 tttaaccgga atcgcatcac tgatcccgcc atccaggtag ttaatgcctt ccagcgacac
     9481 tccgctacga taaaagccag gtatcgcact ggaggcgcga atcacatcca gccagttttg
     9541 tttggttggt aaaaagtaat taggcgcgta gtcatcctga cgacaggcgc acatataaaa
     9601 cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt tgcaacggca tctggcttgc
     9661 ggtggcctcc accagccagt cgagatcgat aagatttcct ccacggacaa agcgcaatgg
     9721 atcgaaaaat tcgcgttttg tggtatagcg catgatgact ttgcgcgcgt aaccgggctg
     9781 attgcagata aacgccgaga ggttctgcgc cccggcagat gtgccgagat aaagatcgaa
     9841 aggattaaac tgcgcgcgca taaactcatc cagcacgcca gccgtgaaaa ttccacgctg
     9901 tccgccgcct tcgcacacca gagctattcg tccaggctgg aacggcctta gcgacaacgg
     9961 cgcaatatta ccaagcgtta caggtattcg ctgccccacc tctgccttcc tgtttttgtt
    10021 gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa aaccgcaaaa gccagtccgc
    10081 tggactggct ttggataaaa ttttcttaat ggtctgaaat ctagggtcgt tttcggccca
    10141 tgaacaaact caccaggaac agaataatcc cgacgacaaa gacaatttta gctgcgcctg
    10201 cagcggtacc ggccagacca ccaaacccaa gtgcggcggc gattaacgcg ataaccagaa
    10261 atatgatgcc ccaacgaaac ataagcgtct cctttaccat agttaatgtc accgcttgat
    10321 atgagcgtga aaatcactca atgcgatatt tttagtgtgg tgcacatcgc gcctcccgac
    10381 aaagttcggg aggacgaatt acgacgaatt acttagtttt cagatcattt ttaacgcttt
    10441 tcacaccatc taccgctttg gcgatacttt cagcacggtc gctctgtgcc tgggaatcga
    10501 cggtaccgga gagctgaacc acgccgtcgg tggtttcaac tttcacatga cgggaaggga
    10561 cgatatcgtc cgccagcagt ttggctttga tttcactggt ggtggcggtg tcacccgcgt
    10621 agcccttcac cgagccttct ttagcgtcgc gaacgtgcag tttgtcgctg acagaggtca
    10681 ccccttcaac gcctttcgcc actttcactg cctcttcggc ctgagcctgg ctttcaacga
    10741 aaccgctcag ggtcacgact ttttgatcgg ttttgacaga gatatcggtg ctcttgatgt
    10801 tgtcatgatc caccagcgcc gccttcactt tcgcggtgat ggcgctgtca tccatgaaat
    10861 taccgacttt attcatagag ctatcgactt tttgccctgc gctttcattg gtagtctgtg
    10921 cgttgttttc cgcgtaggca gagccggtcg cgacggcaga ggtcaacatt acagccagca
    10981 gagttttcga aatcttcagt cttgtcatag tcatcgattt attcctgtat gtttgctcgt
    11041 aatttgagcc tggcaacacg aggttgcatt gctgaatagg gagagacttc accctctaca
    11101 gaagtcaatg gtcgccatca caaaagcgat gggtgatgaa taacgaccat tacagcctct
    11161 gaatcagtta ttaatatcgg tagaatgaca atcgacggct ttagatactg atatctacgc
    11221 attgaacggt atttaacgcc gtcagaaatg tcattacttt gttaaatata gttcacaatt
    11281 ttgaaaccgc tcgggatatc agcgaaaaca taagcaaaag tgaatgtttt aagaacattc
    11341 cgtaagcggc taataaggaa gggaaattga cagggcgcag cggctaccgc gccctggagg
    11401 caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg ggataacgtt cctgcgccag
    11461 gcgcaggttg accatgctgg tagcgatgta agcgaggtta tcgccgccat caagcgccag
    11521 ttggctttcg ttcttacgct tgaactcttc gaatttcttc gcgtccgcac attctaccca
    11581 gcgggcagtg gcaacgttga ctgattcata tactgcttca acgttgtatt cgctcttcag
    11641 gcgcgatacc accacatcaa actgcagcac accaacagca ccaacgatca aatcgttgtt
    11701 ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa agctgtacca gccctttgag
    11761 cagctgtttt tgcttcagcg gatctttcag gcggatacga cggaacagtt ctggtgcgaa
    11821 gttcggaata ccggtgaact tcatcatctc accctgggta aaggtgtcgc cgatctgaat
    11881 agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac gcttcttcaa cgtgcgaacg
    11941 gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca tctttcgcag tacgcacctg
    12001 gcgcagcttc atgccttttt catatttacc ggatactacg cgcataaacg ccacgcggtc
    12061 gcggtgtttc gggtccatgt tggcctgaat tttaaatacg aagccggtaa atttgtcttc
    12121 gctcgcctct acggtacggg tatcagtctg acgcggcatc ggcgcaggag cccactccac
    12181 caggccatcc agcatatgat caacaccgaa gttacccagc gcagtaccga agaataccgg
    12241 agtgatttcg cccgcaagga acagttcttt gtcgaactcg ttagacgcgc ctttcaccag
    12301 ttccagttcg tcacgtagct gctgtgccag atcttcacca accgcagcgt cgagatccgg
    12361 gttattcagc cctttaacaa tacggacttc ctgaatcgtg tggcctttac cgctctgata
    12421 gagataggtt tcgtctttat aaaggtggta aacgccttta aacagcttgc cgcagccaat
    12481 cggccaggtg atcggtgcgc agccgatttt cagctcgttc tcaacttcat cgagcagttc
    12541 catcgggtcg cggatatcac ggtcaagttt gttcataaag gtgaggatcg gcgtgtcgcg
    12601 cagacgggta acttccatca gcttacgggt acgatcttca acaccttttg cagcgtcgat
    12661 aaccatcagg cagcagtcca ccgccgtcag ggtacgatag gtatcttccg agaagtcttc
    12721 gtgccccggg gtgtcgagca ggttaaccag gcaatcgtga tacggaaact gcatcacaga
    12781 cgtagtaatg gagatcccac gctgcttttc catctccatc cagtccgact tagcgtgctg
    12841 gttggaacca cggcctttta ctgtaccggc ggtctgaatg gcctgtccga acagcagcac
    12901 cttctcggtg atggtagtct taccggcatc cgggtgagaa ataatggcaa aagtgcggcg
    12961 cttcgccacc tcttgcaaat aaggagacaa cgtcataatt ctttcttctt gagtaagcgg
    13021 catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat tttacccatc aacggggggg
    13081 aggcaatcag tgtttacaca ggagctgctc cagttcgtgc aacgaagaaa cggtccaggt
    13141 gggcgcgatg ccttctggtt gctcgcgatg gtgcgcattc agccagcagg tcgcaagccc
    13201 ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg ccaaccatca gcacgcgtga
    13261 acggtcagga ttgcccgcct gttccagcgc ataatcgaaa attttcttat tcggtttggc
    13321 aacgccaact tcttcggaaa tcaccagcaa atcgaagtaa tcacgcaggc ccgtgcgttc
    13381 cagacgcact tgctgcaagg cgctaaagcc gttggtgatg atgccgattt tggcgttgcc
    13441 acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc gtgcagattt ccgccatcgc
    13501 attaataaag gcatcgttaa gcaagcctgc ttcaactttc agccgttcgg cccagctctc
    13561 aaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg ttttgataat ccacccacag
    13621 tggcttgtta acggcctgat agtcctgaaa atcttcagcg gtaaaggtga cgctgtaatc
    13681 aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta aacagcgttt catcggcatc
    13741 aaagaaaatc cagtcccact tcattattcc accttgtctt acatactgat tggcaacgcc
    13801 atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt aattgcggcg aatcgtcgcc
    13861 tcgttaaagc ctaaactttc gtacagggca atggcggcag cgtttgaagc acggacttcc
    13921 agccatagtg tcgccacgcc gcgtttttcc agttcgtcga tcagatgttc cagcagcgcc
    13981 cttcccaatc cctgacgctg atagtcagga tcgaccgcaa tattgaacaa tgtagcttca
    14041 tccagcacca cttgcgtaat cgcaaacgcc gccattttgc cgttttgcgt taactgaaag
    14101 ttgagataac gctcgccctg gttgctggca aaggtttttt cactccacgg aaaggcgtgg
    14161 gcgcgttgtt caatgtggta agccgccggt aaatcagtcg tttcgaggga agaaatcgtg
    14221 ttcatatgtg caaatttgtt gccataacgc ggcgcgtgcc gttgggtttg cccgtaaatc
    14281 ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat agcggttcgt cagtacccaa
    14341 ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg attttttctg gcgtcagttg
    14401 cagcacctgg tcggggctga cggttaatgc gcgcagaaca tcgctcacta aaggatcagt
    14461 cagggcggga agatcgtttg ccaccatcac cagacggacg tgtgccggga tcgcaatggc
    14521 aatctccccc tgcaacgcgc cagggcgacg cagcgaccac tgggtaatgc ccagttgctg
    14581 taactgccag tctcgtcggg atgtcatagc gaatcgctcc tgttgtcagg ggcgcaaata
    14641 tagcaaattc gtcgataccg cgccaacata tggctataat cgccgccagt atcaattgag
    14701 gagcattcca tgtctgcatt taccccggca agtgaagtct tgctgcgtca cagtgatgat
    14761 ttcgaacaaa gccgtattct gtttgccgga gacttacagg atgacctgcc cgcgcgttta
    14821 gataccgcgg ccagccgtgc tcatacccag caattccacc actggcaggt attaagccgc
    14881 cagatggggg ataacgcccg tttcagtctg gtcgccacgg cggatgacgt cgcagattgc
    14941 gatacgctga tttactactg gccgaagaac aaaccggaag cccagttcca gttgatgaat
    15001 ttactttctc tgctgccggt ggggacggat atttttgtcg ttggcgagaa ccgcagcggc
    15061 gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt tgaataaagt cgacagcgct
    15121 cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc cggtatttga tgccgataaa
    15181 ttctggggcg aatacagcgt cgatggcctg acggtcaaaa cgctgcctgg cgtgtttagc
    15241 cgcgacggtc tggatgtcgg tagccagttg ctgctctcga cgttaactcc gcacacgaaa
    15301 ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt cagttgcctt tgcgcgccat
    15361 tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc cagcggtaga agccagccgc
    15421 gcaacacttg cggccaactg tgttgaaggt gaagtctttg ccagcaacgt cttttccgag
    15481 gtgaaaggtc gttttgatat gatcatctcc aacccgccgt tccacgatgg gatgcaaacc
    15541 agcctggatg cggcgcaaac gctgattcgc ggcgcggtgc gtcatcttaa tagcggcggc
    15601 gagctgcgaa ttgtagcgaa cgccttcctg ccttacccgg acgtgctgga tgagacattt
    15661 ggcttccatg aagttatcgc gcaaaccggg cgcttcaagg tgtatcgcgc cattatgacc
    15721 cgccaggcga agaaaggtta agtatcccgc tgaatactgt gtcggatgcg gcgtaaacgc
    15781 cttatccgac ctacaaaacc tgcacgttag cccttcgtag gccagataag acgcgcccgc
    15841 gtcgcatctg gcatctgcac ccaaataccg tgtcggatgc ggcgtgaacg ccttatccga
    15901 cctacaaaac ctgcacgcta gtttcctgat ggacattttt ccagcaatta cacctccggc
    15961 gataattaac tattgacgaa aagctgaaaa ccactagaat gcgcctccgt ggtagcaatt
    16021 ctttttaaga attgatggta tgcgaaggtg gcggaattgg tagacgcgct agcttcaggt
    16081 gttagtgtcc ttacggacgt gggggttcaa gtcccccccc tcgcaccaaa aaccacgttg
    16141 atattgctcg cactgggcga aggtggcgga attggtagac gcgctagctt caggtgttag
    16201 tgttcttacg gacgtggggg ttcaagtccc ccccctcgca ccaaacgagg cgatatcaaa
    16261 aaaagtaaga tgactgtgc
//
LOCUS       DAEVZA010000111        16178 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEVZA010000111 DAEVZA010000000
VERSION     DAEVZA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.111"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(18..206)
                     /locus_tag="LAF92_003683"
     CDS             complement(18..206)
                     /locus_tag="LAF92_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HBI9792620.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(210..569)
                     /gene="yeaR"
                     /locus_tag="LAF92_003684"
     CDS             complement(210..569)
                     /gene="yeaR"
                     /locus_tag="LAF92_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HBI9792621.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(742..1380)
                     /gene="leuE"
                     /locus_tag="LAF92_003685"
     CDS             complement(742..1380)
                     /gene="leuE"
                     /locus_tag="LAF92_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBI9792622.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1507..2430)
                     /gene="dmlR"
                     /locus_tag="LAF92_003686"
     CDS             complement(1507..2430)
                     /gene="dmlR"
                     /locus_tag="LAF92_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HBI9792623.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2533..3618
                     /gene="dmlA"
                     /locus_tag="LAF92_003687"
     CDS             2533..3618
                     /gene="dmlA"
                     /locus_tag="LAF92_003687"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HBI9792624.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            3869..5479
                     /gene="yeaV"
                     /locus_tag="LAF92_003688"
     CDS             3869..5479
                     /gene="yeaV"
                     /locus_tag="LAF92_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HBI9792625.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5511..6635
                     /gene="yeaW"
                     /locus_tag="LAF92_003689"
     CDS             5511..6635
                     /gene="yeaW"
                     /locus_tag="LAF92_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HBI9792626.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6691..7656
                     /gene="yeaX"
                     /locus_tag="LAF92_003690"
     CDS             6691..7656
                     /gene="yeaX"
                     /locus_tag="LAF92_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBI9792627.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7710..8825)
                     /gene="rnd"
                     /locus_tag="LAF92_003691"
     CDS             complement(7710..8825)
                     /gene="rnd"
                     /locus_tag="LAF92_003691"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBI9792628.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(8907..10592)
                     /gene="fadD"
                     /locus_tag="LAF92_003692"
     CDS             complement(8907..10592)
                     /gene="fadD"
                     /locus_tag="LAF92_003692"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBI9792629.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(10797..11378)
                     /gene="yeaY"
                     /locus_tag="LAF92_003693"
     CDS             complement(10797..11378)
                     /gene="yeaY"
                     /locus_tag="LAF92_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBI9792630.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11418..12113)
                     /gene="tsaB"
                     /locus_tag="LAF92_003694"
     CDS             complement(11418..12113)
                     /gene="tsaB"
                     /locus_tag="LAF92_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBI9792631.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12171..14081)
                     /locus_tag="LAF92_003695"
     CDS             complement(12171..14081)
                     /locus_tag="LAF92_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBI9792632.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14213..14557
                     /locus_tag="LAF92_003696"
     CDS             14213..14557
                     /locus_tag="LAF92_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBI9792633.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            14919..15278
                     /locus_tag="LAF92_003697"
     CDS             14919..15278
                     /locus_tag="LAF92_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBI9792634.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMEPGERTVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(15398..15577)
                     /locus_tag="LAF92_003698"
     CDS             complement(15398..15577)
                     /locus_tag="LAF92_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBI9792635.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            15651..>16178
                     /gene="pabB"
                     /locus_tag="LAF92_003699"
     CDS             15651..>16178
                     /gene="pabB"
                     /locus_tag="LAF92_003699"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="para-aminobenzoate synthase component 1; catalyzes
                     the formation of 4-amino-4-deoxychorismate from chorismate
                     and L-glutamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBI9792636.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQE"
BASE COUNT         3963 a         4243 c         3962 g         4010 t
ORIGIN      
        1 aggtggaaaa ccgcgcctta tatccccgcc ctgaaggacg gggttttacg gcgcaccgga
       61 taagaacgca cggtattcac cagatctttt atcacttcag ccgccacttc tggcaccagc
      121 aaagtcatcg gcgtctctgt ttcataatcg acagaaacgc cattgctgtt attggtgacg
      181 gtcacggtat acgttgcttt gcccatgatt catttcccgt tatgaatgac tttccgttgt
      241 tgcgcacctt ccatcaggac ttcaggagcc acgaagaagt caatgttgaa ataagtatcg
      301 tcagtcatgg cttcaatgtt gtgccacttt tctggaggga acaccgcaaa ctgccccgct
      361 tcgataagga tcacctgatc aggctctgca ctgtgttcat cagcgtagcc gagatatttg
      421 accgccccat gcataacgga aaggcgtggg taaacccccg ggcgcgttcc tttatcaaga
      481 tgacgttcga atattccggc aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta
      541 tgaatataat tctgtgggat ttgcagcatc ctattccctc cttcggggaa tgcgctgaaa
      601 acggtttatt ccagccgttt cagggtacgc ctgataattt gcattttaaa taccatttat
      661 tggttacttt ttagcaccat atcagagaaa aatcagggag gattacaagt gggaagcccg
      721 cgacaacgcg ggctgaatgt atcaggattg cagcgtcgcc agtcgggcgg cgaaacccac
      781 gaacatcaaa ccaatcagtg agttgccaac tttagccagt ttctttttgg tacgtatgta
      841 ctgcgtgaca aaagcaccag aaataatcag gaagctcaaa tagcagaaac tcaccagttc
      901 cagcgtcgtc gccagaataa agaatgaaat tcccgtatgt ggggcattaa catcgataaa
      961 ctgtacgaaa aacgacacat agaacaaaat ggctttcgga ttagtcaggc tcaaaattaa
     1021 cgcgcgttta aaaatggccc cgtattgggg ctcatcggat ttggcctcgt tatttttacc
     1081 cttcagggtc gcgtagagaa ttttactccc cagatagagc aaataaaacg caccaagata
     1141 acgtacaatg ttgaataata tcggggtggt cttaattaat gtcgccactc cagcccatgc
     1201 cagaaacatc aatactgcat cgccaataaa tacaccgcag gccgcaagat aaccgccttt
     1261 cataccgcta ctgacgctat ttttgagtac aaacagggta tttggccctg gcaccaacac
     1321 aataaaaatg gccccaacca gataggtcca gtaattcaga accccgtatt cagcgaacac
     1381 attaacctct ttaattatct ttcgatcatg cgcgattaaa ggtgaatatg ctaaccaatc
     1441 tgtagcggct tagaaaggag aaaatcaggt tttaacccga tatcaacccg ataattgaat
     1501 cattaatcag gcatgctcca gtgaaaaatt cgggtagtgc tcggcaaaat actggcgtaa
     1561 aaattctacc gttatccgca ctttcgctga cgtcgccagc cttgaaacat aaacggccca
     1621 gacgttcgct ggctggtaat attccggtag cacttgcact aaatgaccac tggcaatgtt
     1681 ttcgctaaca tcccaccagg agcgcagagc aatcccctgc ccatcgagac accactgatg
     1741 gacaatttct ccatgattag acgacagtgg gccggtgact ttaatcgcat ggtggccttc
     1801 tttattgcgt aattgccaga caccaaaagg atgatcgcgc tctttaatga ccaggcacgg
     1861 caatgcggaa agatcggtta aatgtttcgg cgcgccatgt tgcgcaataa attccggcga
     1921 cgcgcagaga atgcggtaat tagtcgccag tttgcgggcg attaaattag gggcgatgtc
     1981 atcgccaatg cgaatatcga gatcaacacc ttcattgacc aaatcgacca accggtcttc
     2041 cacatcaaaa cgtaattcca gttgcggata cgccttagcc agcgctgata acgccggagc
     2101 caccacctgt cgtccaaaac caaagctgct gataatacgc agcatcccct gcggcacctg
     2161 acgcacgtca gaaagttcgt ccatcatctg accgacatcc tgcaaaatcc gctgcgccca
     2221 ttcataaatc cgctctcctt cttcggtaat ggtgacgcgg cgggtggtgc ggtgtaacaa
     2281 caccacgttt agcgtttgct ccagcaaggc gatgcgcttg ctgacgaacg ccggtgaaac
     2341 gcccagttct tccgccacgg cggcaaaacc agcccgacga gccaccagca taaagacgcg
     2401 caaatcattc agcagcggta aattattcat gattcgtgtt ttatgtttca ccagttacgg
     2461 ggattaattc cttttcagtc aattatagga tggtgatgtt gtcaattttg atggtcagga
     2521 agtgagaacc caatgatgaa aacgatgcgt attgctgcga tcccgggaga cgggattggc
     2581 aaagaagtcc ttcctgaagg gattcgcgtg ttacaggctg ccgctgagcg ctggggcttc
     2641 gccttgagtt ttgagcaaat ggagtgggcg agctgcgagt attacagcca tcacggtaaa
     2701 atgatgccgg acgactggca tgagcaactt agccgtttcg acgccatcta ttttggtgcc
     2761 gtcggctggc cggacaccgt tcctgaccat atttcgttgt ggggttcgct gctgaaattt
     2821 cgtcgtgaat tcgaccagta cgtcaacctg cgcccggttc gtctctttcc tggcgttccc
     2881 tgcccgctgg cgggaaaaca gcctggcgac atcgattttt acgtggtcag ggaaaacacc
     2941 gaaggcgaat attcctcgct cggcggtaga gtgaatgaag gtacagagca tgaagtcgtc
     3001 attcaggaat cggtatttac gcgtcgtggt gtcgatcgca ttttgcgtta tgccttcgaa
     3061 cttgcgcaaa gccgcccacg taagacgcta acttctgcca ctaaatcaaa cggtttagcc
     3121 atcagcatgc cgtactggga tgagcgagtg gaagcaatgg ccgagaatta cccggagatc
     3181 cgctgggaca agcagcatat tgatattctc tgcgcgcgtt ttgtgatgca gccggaacgc
     3241 ttcgatgtgg tggtggcgtc caatttattt ggcgatatcc tttccgatct tggcccggcc
     3301 tgcaccggca ccattggcat tgccccatcc gccaacctga atccggaacg cactttcccg
     3361 tcgctcttcg agccagtcca cggttccgcg ccggatatct acgggaaaaa cattgctaac
     3421 cctatcgcca cgatttgggc cggtgcaatg atgctcgatt ttctcggcaa tggcgatgag
     3481 cgtttccagc aagcgcataa cggtattctg gcagcaattg aagaagtgat tgctcacggg
     3541 ccgaaaacac ctgatatgaa aggcaatgcc accacgccac aggttgccga cgcgatttgc
     3601 aaaattattt tgcgttaagg tcaaaccagt ttatttgaac cgcgtcactg acgcggtttt
     3661 tttattcgtt ctttgcagta aataacctgc gtcatttcac cttttattgt ttccgtttcg
     3721 tgttttatgg ctttccgtat tcttaattgt ttaatttatg taacatgcaa ataatcaatt
     3781 tgttaaacac aacatacaac tcattaccct gcaaataatt acaactggca aaagccagag
     3841 acgggctttt atgcccagga gatgactcat gatgagcaat gttaagaaaa aagatgtgcc
     3901 gctgataagc atcagcctgg tggccattct tttcatcgca gctgcattaa gccttttccc
     3961 acaacaatcg gccgacgcgg ccaacgcaat atacactttt gttacgcgta cgttaggttc
     4021 cgccgtacag gtattggttt tgctggcaat gggactggtg atttatttag ccaccagtaa
     4081 atacggcaat attcgtcttg gcgaaggaaa accggaatac agcacactct cctggctgtt
     4141 tatgtttatt tgtgccggtt taggttcttc tacgctttat tggggggttg ctgaatgggc
     4201 ctattattat caaacgcctg gattaaatat cgcaccgcgt tcacaacagg cactcgaatt
     4261 tagcgtccct tactccttct tccactgggg catcagcgcc tgggcaactt atacgctggc
     4321 ctcattaatc atggcttatc actttcatgt gcgtaaaaac aaaggtctga gtctttccgg
     4381 cattattgcc gcgattaccg gcgttcgccc gcaaggccca tggggaaaac tggttgattt
     4441 gatgttcctg atcgccactg tcggcgcact gactatttcc cttgttgtca ccgcagcaac
     4501 ctttactcgt ggactttccg cgctgaccgg tttacccgat aacttcaccg tgcaggcatt
     4561 tgtgatcctg ctttccggcg gcattttttg cctaagctcg tggattggta tcaacaacgg
     4621 tttgcaacgt ctgagcaaaa tggttggctg gggcgcgttc ctgctgccat tactggtgct
     4681 gattgtcggc ccaaccgaat ttattaccaa cagcatcatc aatgccatcg gtctgaccac
     4741 gcaaaacttc ctgcaaatga gcttattcac cgatccgctt ggcgatggtt catttacccg
     4801 caactggacc gttttctact ggctgtggtg gatctcatac acccctggcg tagcaatgtt
     4861 tgtcacccgc gtttcccgcg gtcgtaagat taaagaagtt atctggggac tgatcctcgg
     4921 cagcaccgtc ggttgctggt tcttcttcgg cgtaatggaa agctatgcca ttcatcagtt
     4981 tatcaatggc gtaatcaacg tcccacaggt gctggaaaca ctgggcggcg aaacagctgt
     5041 acagcaagtt ctgatgtcgt tgccagccgg taaattgttc ctcgccgcat acctgggcgt
     5101 gatgattatt ttccttgcct cgcatatgga tgcggtggcc tacaccatgg ctgcgaccag
     5161 tacgcgtaat ctccaggaag gtgacgatcc tgaccgtggg ctgcgtcttt tctggtgcgt
     5221 ggtgatcact ctgatcccgc tttccatctt gtttaccggt gcttcgctgg aaacgatgaa
     5281 aaccaccgtc gtgctcacag cccttccctt cctcgtcatt ttactggtga aagtcggcgg
     5341 atttattcgc tggctgaaac aggattacgc cgacattccg gctcatcaag ttgaacatta
     5401 tctcccgcag acaccggttg aagccctgga aaaaacacca gtgctccctg cgggaaccgt
     5461 attcaaaggc gacaactgag cgccatcatc ctaacgataa aggtatccct atgagcaatc
     5521 tgagccctga ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag gcgtggacca
     5581 ttcctgcccg tttttatacc gatcagaacg catttgaaca cgaaaaagag aacgtcttcg
     5641 ccaaaagctg gatttgcgtc gctcacagca gcgaactggc gaatgccaat gattatgtga
     5701 cgcgtgagat cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag gttttgcgcg
     5761 cgttctataa cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa ggaaaagcaa
     5821 aaaatgtgat tacctgcccg tatcacgcat gggcattcaa actcgatggc aatctggccc
     5881 atgcacgtaa ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa ctggttccgg
     5941 tgcgtctgga agaatatgcc ggattcgtct tcatcaacat ggaccccaac gccaccagcg
     6001 tagaagatca gttacccggt ctgggcgcga aagtgctgga agcctgcccg gaagtccacg
     6061 atctgaaact ggcggcccgc tttaccaccc gcacgcctgc caactggaag aacattgtcg
     6121 ataactatct cgagtgctat cactgtggtc cggcgcatcc aggtttctcc gactccgtac
     6181 aggttgatcg ttactggcac accatgcacg gtaactggac gctgcaatac ggtttcgcca
     6241 aaccgtccga acagtcgttt aaatttgaag agggtacgga tgcggcattc cacggtttct
     6301 ggctgtggcc gtgcacgatg ctgaacgtca ccccgatcaa agggatgatg acggtcattt
     6361 atgaattccc ggtggattct gaaactaccc tgcaaaacta cgatatttac ttcaccaatg
     6421 aagagttaac cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg ttccgtccgg
     6481 aagatttacg tctggttgaa agcgtacaga aagggctgaa atcgcgtggc tatcgtggtc
     6541 aggggcgcat catggccgac agtagcggta gtggcatttc cgaacatggt atcgcccatt
     6601 tccataatct gctggcgcag gtgtttaagg actaatgaca tcggcggcgg tattttccgc
     6661 cgctgggctg atttttgatg gagtacagca atgtcagact atcaaatgtt tgaagtacag
     6721 gtgagtcagg tggaaccgct taccgaacag gtgaaacgct tcacgctggt ggcaaccgat
     6781 ggcaaaccgc tgcctgcgtt caccggagga agtcacatca ttgtgcagat gagcgatggt
     6841 gataaccagt acagcaatgc gtattcacta ctgagttcgc cgcatgacac ctcttgttat
     6901 cagattgccg ttcggctgga ggaaaactcg cgcggcggtt cccgcttttt gcatcagcag
     6961 gtaaaagtgg gcgatcggtt aacgatttca acgcctaata acctgtttgc gctaattccc
     7021 tcagccagaa agcatctgtt tatcgcgggc ggtattggta tcaccccttt cctgtcgcac
     7081 atggcagagc tgcaacacag cgacgtcgac tggcagctac attactgctc gcgaaatcca
     7141 gaaagttgcg catttcgtga tgagctagtc cagcatccgc aggctgagaa agttcacttg
     7201 catcactcat caaccggaac acgactggaa ttagcgcgat tattggcgga tatcgaacct
     7261 ggcacacacg tttatacctg tggtcccgag gcgctaaatg aagcggtaag aagtgaagct
     7321 gcgcgtctgg acatcgtcgc cgatacgctg cactttgagc aatttgctat cgaagacaaa
     7381 accggcgatg cctttaccct ggtgcttgcc cgttccggaa aagagtttgt ggtgccggaa
     7441 gagatgacta ttttgcaggt tattgaaaat aataaagccg cgaaagtgga atgtttatgt
     7501 cgtgaagggg tatgcggaac ctgcgaaaca gcaatactgg aaggtgaagc tgaccatcgg
     7561 gatcagtatt ttagcgatga agagcgtgcc agccagcaaa gtatgttgat ctgttgttcg
     7621 cgtgcgaagg gtaaacgcct ggtgttggat ttgtagtttg ccaaagccgg atgtggcgct
     7681 gagcgcgccc agtccggctt cggaagattt tactgcggat attcctgcaa taaattgtgt
     7741 aatgcttccg ccatcagctc accacgccag ccggaaatca gctccggcaa attgttctgc
     7801 ggtttcagtt tccagtgcca gttcagcagt tggttgattt gccgacgcga tgccagcaat
     7861 tcggcgctga tcttatgcgt ttcgctcacg tcagtaatca gcgacttaat cgctttaaac
     7921 gctttacgat aaccaggcat gtccatcagg ttaagcatcg gctgcggtaa ggcctcttcc
     7981 ggcaatgtct gcgctttttc caccagcgcc agcagcgttt taccgtgaaa gcggatttcg
     8041 ctaccggata aacccaggct gtccagttcg cctaaacttc ccggcatata acgcgcgacc
     8101 gaccacaaat gctcttcacg cacgacaaag ttcaccgcca gatcacgctc tcgcgccttg
     8161 cgcagtcgcc agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg taactgccag
     8221 gcattggtga tatcacgcca ggcatcttcc ggcgcaacga cttcctgacg acgcatttgc
     8281 atcaggcggc attcatccag cgccgcaggt agccagccgg aggcctccgt ttctaccata
     8341 agcttggcgg tgatcggtaa cagataccag acatccgccg ctgcgtattc acactgacgt
     8401 tcggtcagcg gtctggccag ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg
     8461 gaatactctt ccaccatgga agcgaaaccc catgacatcg ggcgtccgca gaaggcagca
     8521 aggatttgcg tgtcaatcaa gggttgtggt aattcgccaa agacattgag gaacacttcc
     8581 agatcttcac tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag gatcgctttc
     8641 agcggtgacc agtcggtgat cccgagagga tcaattagcg ccagatgctc gccatcgaaa
     8701 agttgaatca accccagctg cgggtaataa gtgcgcgtac gaacaaattc agtatccagg
     8761 gctatcgccg gaaaggcacg gacggcttca cacaaagaag ccagcgcatc gtccgtggta
     8821 atcatttggt aattcaaatt gttttctctt tagtgggcgt caaaaaaaac gccggattaa
     8881 ccggcgtctg acgactgact taacactcag gctttattgt ccactttgcc gcgcgcttcg
     8941 tcacgtaatt ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac
     9001 tccaccagct tcggtacttt gtaacccgtg agctgacggc ggcaaaaggt caccagtgac
     9061 tcttcggtaa gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag
     9121 ccggaaggta cgccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct
     9181 tcaatctcgt tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg
     9241 acaatgcgca ggaacccttc ttcatccatc accgcgatgt cgccggtgtg taaccagcca
     9301 tttttgatga tctcatctgt agcatccgga cgctgccagt aacccagcat cacctgcggt
     9361 cctttgacac aaagctcacc cggttgaccc ggtggtactt cattatcatc atcatccacc
     9421 agtttggctt ccgtcgacgg caccggcaaa ccgatgctac cactatgata atcaatatca
     9481 tatgggttaa cgctgaccag cggcgcacac tcggtaaggc catagccttc cagcagatac
     9541 tgtcctgtca gtttcaccca acgctctgcc accacttgct gcactggcat ccctccgcct
     9601 gcggaaagat gcagactgga gaaatccagc tgctggaact ctttattgtt cagcaacgca
     9661 ttgaacaagg tgttaacgcc cgtgatagcg gtaaacggat atttcgctaa ctcttttacc
     9721 aaccctggaa tatcgcgcgg gttagtgata agcaggttct gcccacccag ttcgataaac
     9781 agcaggcagt taatggtcag ggcaaaaatg tgatacagcg gcagcgccgt caccaccagc
     9841 tctttgcccg gatgcaacag cggaccatag gtcgcgttaa cctgttccag gttcgccagc
     9901 atattgcggt gagtcagcat cgcgcctttc gccacaccag tggtgccgcc ggtgtattgc
     9961 agaaaagcta aatcttccgg caccagttcg ggtttgacgt actgcatccg gtagccgtta
    10021 tgcagtgcgc tacgaaatga aatggcatct ggcagatggt atttcggcac caaacgcttg
    10081 atgtatttaa caacgaaatt gactaccgtg ccttttgccg tagatagctg atcgcccata
    10141 cgggtcagaa ttacgtgctg aacggcggtt ttatcaacca ctttttccag tgtgtgagca
    10201 aagttagaca cgataacaat cgccgatgcg ccgctatcgt taagctgatg ctcaagctca
    10261 cgcggggtat acaacgggtt aacgtttacg acgatcatcc cggcacgcaa aatgccaaac
    10321 agcgccaccg gatattgcaa taaattaggc atcatcaacg caacgcgatc gcctttcttc
    10381 agccccaacc cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc cagcttgcgg
    10441 aaggtcatta cctcccccat attcacaaac gcaggttgat cggcgtagcg cgcgaccgac
    10501 tgctcaaaca tatctaccag agattgataa cggtcagggt tgatctccgt cggaacgtcc
    10561 gcgggataac ggttaagcca aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc
    10621 gtcatcgcaa ccccaaatga tatacatgcc gttaacataa tattaactca tcataccagc
    10681 ttgataatta cccaacgaaa aggttgcgaa gcgcgtcact atttattttt atctttaccg
    10741 taagaatgca gaaacagcgg accagccgct gtttcttttt ctttaaaaac aagcaattat
    10801 tcagttacaa ctgtttgtac tctcgcgggg ccgggattat accagcccca tccgccgtag
    10861 ccatagggcc agccacgacc gccataaaac catggatcaa tcggctgagg cggcataatc
    10921 acctgctggg ttaaatgcca acgtttgtaa cccgttactt gcatcaccat aaatttatag
    10981 ggcgtattgc cgattttgcc gtcaaccgca ccagtgattg gcccgactac cgtaaccagt
    11041 tgtccacgga aatccaccgg gtccagaaaa ccgttcacat cggcataaat gcgaccgcga
    11101 gaaggttctc ccagcgtcgg tctggctccg ctgtccagcg gtacagtagc aatttccagg
    11161 cgggttttcc cttgctggtt ttgtaccgca accactttgc caccaaagcg tgcctcctga
    11221 ccaacgtaca gctgcggcgc actcatcacc cgaactaaat cttgttgcgg cgtgggactg
    11281 ctgcctttaa tggcgtccgg cacagtgaca caaccgctca gcattagcgc aaacgtacct
    11341 gccagtatgc ctttgataac attcttttga accgccatga tgcgactcct ttttttctca
    11401 gggcatactc ttaagattca ttctttgccc ggaagtttct tccatgcgac gttgttacgt
    11461 aaataaaccg gttcggcatg ttccaccgcc accgttttac cctcggcaaa catctgacac
    11521 gcaatcggca gcatatcttc agcagcaggc agtaacactt cgccatcgcg caaaaccagc
    11581 ccgctctctt taccgagatc cggccaggct tgccagcccg tccctaccgt cacccattcg
    11641 ccggaaagct gttgcattcg ttcatggacg atttcgggtt tgagtacggc ttcggtttct
    11701 tcaccgtgcc agataccgtt ttcatcacgc tgatattcgg cccagtaaac ttcgcccatt
    11761 cgcgcgtcaa tggctgacag cacgcgggtt gcgccgtttt tgcgccacgc cccttgcgcc
    11821 atcgtcatta gcgtggagac gccaatcatc ggtaattccg cgccaagcgc cagcccttgc
    11881 gcgatgccaa taccaatgcg cacgccggta aagctaccgg ggccgcgccc gtaagccaga
    11941 gcgtttatgt cagtcaggga agttccgctg gtggtcagga tatcctgcac catcggtaag
    12001 attcgttgag tatgttcacg agggcaaagc tcaaaatgag cgttgacagt accgtcgttc
    12061 cacagggcga cagagcaggc ctctgtcgcg gtatcgatag ccagaattcg catgggtctt
    12121 cgtgcttttt tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa ttacctggag
    12181 gatggtatcg caaggaaacg aaccgcacgg gcaatgtcac gggtgcgcgg cgcgggcggc
    12241 agactggcga gaaacgtcgc gccgtaaggg cgcatcacca gccgattgtc acaaatcacc
    12301 aaaaccccgc gatcgtcggc gtcgcgaatc agtcgcccta ccccctgctt gagagtaatc
    12361 accgcatccg gcagttgtac ttcatcgaac gggtcgccac cgcgcaaacg gcaatcttcc
    12421 atgcgcgctt ttaacagcgg gtcatccggt gaggtaaacg gcaacttgtc gataatcacc
    12481 aatgacaatg tatcgccacg cacgtccacc ccttcccaga aactactggt tgccacaaga
    12541 agcgcgttac cggcgctgac aaattgctgc aacagttgcc ctttgctggt ttccccctgc
    12601 aacaaaacgg gaagcgtcat ggtagcgcgg aactgctcgg ccagatcacg catcatggcg
    12661 tgcgaggtac aaagcataaa acaacgaccg ttgttagctt cgatgatcgg tcgcagcatt
    12721 gccgctaact ggcgagcaga acctggctgg ttggtttgcg gcagattgcg cggcacacag
    12781 agtaacgcct ggcggctgta atcaaacggg cttggcaata gcaacgactc cgcctgttct
    12841 atcccaagcc gcgaggtgaa atgatgcaga tcgtcgttca ccgacagcgt tgctgaggtg
    12901 aagatccagc taccaggctt ttgcgccatt aactctttga atttatccgc cacgctgagc
    12961 ggcgtgagag ccagagtaaa atggcgcgaa gtgcattcgt accagtagct gtagcccggc
    13021 tgattgatct cttttagccg cttcagccgt gtgcgataca acgtagcgcg ctcaaatgcc
    13081 gcatccagca aggcggaacg ccccagtgac agtttcgcca cgtcataaca aagttccagg
    13141 gtgtcatcga gcagtaaaaa tgcccgctga atttgcggat tagctaacag ctcacgcagg
    13201 ttaccgcgat aacctggctc accgagttgc agacgaaaat cctgcgcact ctgggcaaga
    13261 cgatcagcgc acttttgtaa ctgctgggtg tcttttaatt cggtgcggta agcgatggtg
    13321 atgtcttttg ccaggtcgag cagttgtcga ctggagagtg actgaccaaa atactggctg
    13381 gcaatgtccg gtagctggtg ggcttcgtcg aagatcatga cgtccgcttc cgggatcagt
    13441 tcgccaaatc cactctcttt aaccaccata tccgccagaa agagatgatg gtttaccacc
    13501 accacatcgg cgtccatcgc ttttttacgt gctttgacca caaagcaatc tttatacatc
    13561 gggcagtcgc tgccaagaca gttgtcgttg gtgctggtga ccagcggcca cgcctgtgaa
    13621 tcttccgcca cgctgacgca ggtgctgata tcaccatcga ctgtttgatt agaccaggag
    13681 cgcagcagga tcacatcgct taagatttgt accggcagat cgccccccgc cagcgcctgc
    13741 tgttcgagac gttcgaggca gaggtagttt gagcgccctt tcagcagcgc cacgttgccc
    13801 gtatatttca atgcctttga gactgttggc aaatcgcggc tgtagagctg atcctgcaac
    13861 gcttttgagc cggtcgagat aatgactttc tttttcgccc gcagcgcagg agccaggtaa
    13921 gcgtaggttt tgcccgtacc ggttcctgct tccaccacca gcggctggcc tttttctatc
    13981 gcctgggtga cggctaccgc catctgtcgc tgtggttctc gcggcttaaa gcctggtatc
    14041 gctttcgcca gctgaccgtc tggtgcaaaa tcgtccgtca cactaccccc tgttgatttg
    14101 aacagggatt atgtcaggat gagggcgctt tcgccagttg aagtggtgac ggcgacctca
    14161 cattgtggca gtctttgcag cacgaaaatg gaagtataat gaggacaaaa tgatgactat
    14221 cgttcgtatc gatgctgaag cccgctggtc tgatgtagta atccacaaca acacgctcta
    14281 ctacactggt gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg ccaacacgct
    14341 ggcacagatt gacgccgtgc tggaaaaaca gggcagcaat aaatcgagca ttctggatgc
    14401 caccattttc ctggccgata aaaacgactt cgcggcgatg aataaagcgt gggatgcctg
    14461 ggttgtcgcg ggtcatgcgc cggtgcgctg cacggtacaa gcgggtttga tgaacccgaa
    14521 gtataaagtt gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc aaaataagtc
    14581 agacgataat ttatcgataa tactggtcgg ttttacataa atcgaccaga gaataagata
    14641 ttactgttga tgcttctgct aattcattat ttatatttat aatttcaatt ttatctataa
    14701 aactaactat ataaataaac ataaacatgc cgttgtgttc gccctttgat tagcaactct
    14761 ggttttcttt cttagctgaa caatctggtt atagcgtgcg gactgaccgt taattttctc
    14821 catcttactg gataacacac catgagctgc tacctttaaa acacctgaat gtcaacaggt
    14881 taactcgtgc catatcgtta taatcccttt gatattagat gcaaattaag gtcatatata
    14941 gccttatcga taacatggtt aattttaagg ataagaatat gcctgctgta atagataaag
    15001 ccctggattt cattggtgcc atggatgtat cagcgccaac accaagttcg atgaatgaaa
    15061 gcacagcgaa gggaatcttt aaatatttaa aagaactggg agtacccgcg agtgccgctg
    15121 atattacggc gcgagctgac caggaaggct ggaaccctgg gttcacggaa aaaatggttg
    15181 gatgggcaaa aaaaatggag cctggtgaac gcactgtaat taaaaatcct gaatactttt
    15241 caacatatat gcaggaagaa ctaaaagcac tggtctgagt ttaatttata tcagcataaa
    15301 tgggtcagga cgcttttaat cacatataaa aagcgtcctt tttctcacaa tcaaataatc
    15361 atcggattct ataacgaaat cgatacggat tagtgtgtta ctcgtcttca tcctcaaaac
    15421 gcgccacgat tcgctcgccg gagtggttgg cacgcagctc ttctgccacc agcgcgattg
    15481 cctggccgct gctcatccct tgtgccatca gttcctggat ccgctcgaca gctttttgct
    15541 gctgttcatg ggtgagtgaa ggtaaacctg caaacattgt taactcctgc taaattgttg
    15601 gcgctaatta tttcatgcta cccggcacat agccagtaga gtcaggactg atgaagacgt
    15661 tatctcccgc tgtgattact ttaccctggc gtcaggacgc cgctgaattt tatttctccc
    15721 gcttaagcca cctgccgtgg gcgatgcttt tacactccgg ctatgccgat catccgtata
    15781 gccgctttga tattgtggtc gccgatccga tttgcacttt aaccactttc ggtaaagaaa
    15841 ccgttgttag tgaaagcgaa aaacgcacaa cgaccactga tgacccgcta caggtgctcc
    15901 agcaggtgct ggatcgcgca gacattcgcc caacgcataa cgaagatttg ccatttcagg
    15961 gcggcgcact ggggttgttt ggctacgatc tgggccgccg ttttgagtca ctgccagaaa
    16021 ttgcggaaca agatatcgtt ctgccggata tggcagtggg tatctacgat tgggcgctca
    16081 ttgtcgacca ccagcgtcat acagtttctt tgctgagtca taatgatgtc aatgcccgtc
    16141 gggcctggct ggaaagccag caattctcgc cgcaggaa
//
LOCUS       DAEVZA010000112        15570 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEVZA010000112 DAEVZA010000000
VERSION     DAEVZA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.112"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..183
                     /locus_tag="LAF92_003700"
     CDS             <1..183
                     /locus_tag="LAF92_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI9792637.1"
                     /translation="ENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
     gene            227..334
                     /locus_tag="LAF92_003701"
                     /pseudo
     CDS             227..334
                     /locus_tag="LAF92_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(318..956)
                     /gene="ytfB"
                     /locus_tag="LAF92_003702"
     CDS             complement(318..956)
                     /gene="ytfB"
                     /locus_tag="LAF92_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HBI9792638.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDMPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1175..1795
                     /gene="fklB"
                     /locus_tag="LAF92_003703"
     CDS             1175..1795
                     /gene="fklB"
                     /locus_tag="LAF92_003703"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI9792639.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2104..3516
                     /gene="cycA"
                     /locus_tag="LAF92_003704"
                     /gene_synonym="dagA"
     CDS             2104..3516
                     /gene="cycA"
                     /locus_tag="LAF92_003704"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBI9792640.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3561..4223)
                     /gene="ytfE"
                     /locus_tag="LAF92_003705"
     CDS             complement(3561..4223)
                     /gene="ytfE"
                     /locus_tag="LAF92_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBI9792641.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4331..5296)
                     /locus_tag="LAF92_003706"
     CDS             complement(4331..5296)
                     /locus_tag="LAF92_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI9792642.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5404..6264)
                     /gene="qorB"
                     /locus_tag="LAF92_003707"
     CDS             complement(5404..6264)
                     /gene="qorB"
                     /locus_tag="LAF92_003707"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBI9792643.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6353..6733
                     /locus_tag="LAF92_003708"
     CDS             6353..6733
                     /locus_tag="LAF92_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI9792644.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6862..8805)
                     /gene="cpdB"
                     /locus_tag="LAF92_003709"
     CDS             complement(6862..8805)
                     /gene="cpdB"
                     /locus_tag="LAF92_003709"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBI9792645.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8995..9735
                     /gene="cysQ"
                     /locus_tag="LAF92_003710"
     CDS             8995..9735
                     /gene="cysQ"
                     /locus_tag="LAF92_003710"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBI9792646.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(9725..10282)
                     /locus_tag="LAF92_003711"
     CDS             complement(9725..10282)
                     /locus_tag="LAF92_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBI9792647.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            10607..10813
                     /locus_tag="LAF92_003712"
     CDS             10607..10813
                     /locus_tag="LAF92_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBI9792648.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(10875..12218)
                     /locus_tag="LAF92_003713"
     CDS             complement(10875..12218)
                     /locus_tag="LAF92_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBI9792649.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(12540..13178)
                     /gene="msrA"
                     /locus_tag="LAF92_003714"
     CDS             complement(12540..13178)
                     /gene="msrA"
                     /locus_tag="LAF92_003714"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBI9792650.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            13384..15117
                     /gene="tamA"
                     /locus_tag="LAF92_003715"
     CDS             13384..15117
                     /gene="tamA"
                     /locus_tag="LAF92_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBI9792651.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            15114..>15570
                     /locus_tag="LAF92_003716"
     CDS             15114..>15570
                     /locus_tag="LAF92_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation and assembly module TamB"
                     /protein_id="HBI9792652.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTV"
BASE COUNT         3675 a         3923 c         4102 g         3870 t
ORIGIN      
        1 gaaaatcgcc tgtcacaaag taaattcaga tgccaggtat gtggatatac agcgaacgcc
       61 gatgtaaacg gcgctcgtaa cattttagcg gcggggcacg ccgttcttgc ctgtggagag
      121 atggtgcagt caggccgctc gttgaagcag gaacccaccg aaatgattca ggcgacagcc
      181 tgaacgaagc agggatccac gtccttcagg gcgtggagga tgtcaacata tcagtatggt
      241 ttccccccag gcactggaag ccagacgtaa ggtcatcaaa gagacaatcc ttagctggct
      301 tttggttgac ccttcatcta ccgcgcacga ataaaactgc catccggctg gcgggtaaac
      361 aggacctgtt gattattccc cgtatcaatg gttaagcccg tcaccacgcc gctggcgttc
      421 tggcggattt tcaccatttg cccgttttgc aaattactca gcggcttacc cgcgccttct
      481 acctgcgcca ttgcatagac atcggtagcg ggcagtccgt ggtcgcggaa cagctgcgcc
      541 atcgttttac cgggttcgac acggtaagag cgccattgat tatcaattcc gctatctggc
      601 tggaatggtt gcgtttgggt agtttgcggc tgctcttctg gctggccttc ctgtataggt
      661 tctggagcga ccggagccac ctgatcagga tcattttgtg gggtgactaa ttgcgcccgc
      721 agctgttctt ctgtcggcgg ttgtgattgc gactgaatat ctaactgcgc ttcacgcgtc
      781 accaccggcg cgttgggcat atcatcagat ggaagcagaa atccgaccac caatacaatg
      841 gcggcaataa tgatcccccg acgatgcatc ggcggcagcg ggtccatgaa gcgaaaattg
      901 tccggcgcgt gccagacttt ctccagggtt ggttttagtt caaagcgccc gggcatggtt
      961 tcctcctgct ccgcgtcttg ttcctcaatc atagcctatg aataagctaa cgctatgatg
     1021 tccgtggtaa acccgccttt attatattca tacgggatat tgctattgtt tctttttccc
     1081 cgggatttgt catcattcct gcgacaaagt tttacccaaa gaagtgtggc tgatatgctg
     1141 cccgctactt ttatacccta agaaaggaaa tacgatgacc accccaactt ttgacaccat
     1201 cgaagcgcaa gcaagctacg gcattggttt gcaggtaggg caacaactga gtgaatctgg
     1261 cctggaaggg ctgctgccag aagcactggt tgcaggtatt gccgatgcgc tggaaggcaa
     1321 acatccggct gttccggttg atgtggtgca tcgcgcgctg cgtgaaatcc acgagcgcgc
     1381 cgatgccgtt cgtcgtcagc gtttccaggc gatggctgct gaaggtgtga aatacctgga
     1441 agaaaacgcc aaaaaagaag gtgtgaatag caccgaatct ggcctgcaat tccgcgtgat
     1501 caaccagggt gaaggcgcaa ttccggcacg taccgaccgc gttcgtgttc attacaccgg
     1561 taaactgatc gacggcaccg tgtttgacag ctccgttgct cgtggtgaac ccgctgaatt
     1621 tccggttaat ggcgtgatcc ctggctggat tgaagcactg actctgatgc cggtaggttc
     1681 taaatgggaa ctgactatcc cgcaggaact ggcatatggc gagcgcggcg caggcgcatc
     1741 catccctccg ttcagcaccc tggtgtttga agtcgaactg ctggaaatcc tctaagcagc
     1801 gcattctgtt cccctcgaac cagaggggag caggtattca gcaataaacc cttcagtttg
     1861 ccaaacggcg ctattttgtg ttgcaaagac cgcgtaagcg tgtatttttg tgagctgttt
     1921 cgcgttatca ccgtgatatg acactcactt taaacataaa attaacatta gatctaaatc
     1981 ttagtattca tcccgcgtat tgttacctaa tatcgatgag tcccgataca gattcgtcgt
     2041 atcatagact gactaaaggc cgtagagcct gaacaacaca gacaggtaca ggaagaaaaa
     2101 aacatggtag atcaggtaaa agtcgttgcc gatgatcagg ctccggctga acagtcgcta
     2161 cggcgcaatc tcacaaaccg acatattcag cttattgcca ttggcggtgc cattggtacg
     2221 gggttgttta tggggtctgg caaaacgatt agccttgccg ggccgtcgat cattttcgtt
     2281 tatatgatca tcggttttat gctctttttc gtgatgcggg caatggggga attgctgctt
     2341 tcgaatctgg aatacaaatc ttttagtgac ttcgcttccg atttactcgg gccgtgggca
     2401 ggatatttca ccggctggac ttactggttc tgctgggttg taaccggtat ggcagacgtg
     2461 gtggcgatca cggcttatgc tcagttctgg ttccccgatc tctccgactg ggtcgcctcg
     2521 ctggcggtga tagtgctgct gctgacgctc aatctcgcca ccgtgaaaat gttcggtgag
     2581 atggagttct ggtttgcgat gatcaaaatc gtcgccattg tgtcgctgat tgtcgtcggc
     2641 ctggttatgg tggcgatgca ctttcagtca ccgactggtg tggaagcgtc attcgcgcat
     2701 ttgtggaatg atggcggctg gttcccgaaa ggtttaagtg gcttctttgc cggattccag
     2761 atagcggttt tcgctttcgt agggattgag ctggtaggta caaccgctgc ggaaaccaaa
     2821 gatccggaga aatcactgcc gcgtgcaatt aactctattc cgatccgtat cattatgttc
     2881 tacgtcttcg cgctgattgt aattatgtcc gtgacgccgt ggagttcggt agtcccggag
     2941 aaaagcccgt ttgttgaact gttcgtgttg gtgggtttgc ctgcggctgc cagcgtgatc
     3001 aactttgtgg tgctgacctc tgcggcgtct tccgctaaca gcggcgtctt ctctaccagc
     3061 cgtatgctgt ttggcctggc ccaggaaggt gttgcaccga aagcgttcgc taaactctct
     3121 aagcgcgcag tacccgcgaa agggctgacc ttctcctgca tctgtctgct cggcggcgtg
     3181 gtgatgctgt atgtgaatcc cagcgtgatt ggcgcgttca cgatgatcac gacggtttcc
     3241 gcgattctgt ttatgttcgt ctggacgatt atcctttgct cgtacctggt gtatcgcaaa
     3301 cagcgtcctc atctgcatga gaagtcgatc tacaagatgc cgctcggcaa gctgatgtgc
     3361 tgggtatgta tggcgttctt tgtgttcgtg atcgtgctgc tgacactgga agatgacact
     3421 cgccaggcgc tgctggttac cccgctgtgg tttatcgcgc tggggttggg ctggctgttt
     3481 attggtaaga agcgggctgc tgaactgcgg aaataaccgc attatcatgc tggatggcgc
     3541 aatgccatcc agcttttaga tcactcaccc gctagggcgc gtgggaacag tacattgttt
     3601 tccagactga tgtgatccat caggtcatca atcagttcat taatgccgtt atacatcgct
     3661 ttccaggtgg tgcaggcttc tggcggcggt gtgacgttat tggtagtgtg tttaatcact
     3721 tccagcagtt cgcccgcttc atcgtgctcg ctttccatta cgctgattgg ccccattgcc
     3781 tggctgccca tgccttgttt gatcatcggg aagaggatct gctcttcttt catcatgtgg
     3841 ctggaaagct cttcatgcag catagtcagg tattttgtca gcccttttgg cacgctcggt
     3901 ttgtcggcgt gaacgcgctc gactttagtc gcttgcagga tcagctccgg cagctgctcg
     3961 cggtgacgat cgtggtagcg cacgatgata tggtcaatga tttctgccag cggggcgcta
     4021 cgccagtctt tctcaatcgg ttgttcagca agctttgcca gttcagcttc aatgacctca
     4081 acatccagtt ctttacgtgc cgccgcgcgc gccagcgtct gcttaccgcc acagcagtaa
     4141 tccatatcat atttacgaaa cagagccgaa gcgcgaggga tagagagcgc cagttcacct
     4201 aaaggttggt cgcgataagc catagctgat acctcattct taataagata agatgtattt
     4261 taaatgcatc tttaaggcaa aagctataac ccttacgtag cagaggctta tttaactcac
     4321 tgccagcgtg tcaactttct gagacagatt cagttaaagg tttttctggt tttgctctga
     4381 ccgcaatgac cacgccaatc actaacagcg cgataccgct cagcgtcatt agcggcggca
     4441 tttgctggcg gagtaaaaag gtgtacagca aacctgccag cgtttcgaaa acaatcagcg
     4501 gcccgagaat cactgtcggt aatcgctggc tggcgacgtt ccagcagagt gcgccaaccc
     4561 atgagcaaag cacggctatc gcaaccatca gactaataaa caccagcgga cgggggccaa
     4621 aaggtaagga gaagtccggc gtttgtatat tcagccagta acaggcgacg agatagccga
     4681 tgagagaaac cggcagcgtg accagcgcct gcgccgtcgc ccacatcatc ggatgtttgt
     4741 cgggattttc ccgcagccag cgggcgttgc gcagggcata ccatgcccag cagaccacgg
     4801 aaactaacgc tagcacgatg ccagaggtat aacgtgccca gtcaaaatcg gggagtccgt
     4861 ggtttaactc agcaatattc acactcgcca ggccgatgcc aatacaaatc agtgccgggg
     4921 cgagttttcc ccacgcgagt ttgccgtcgc gctggctata aagcagattg gcaaagacag
     4981 gaatgaccac cggcagggtg ccgataatca tcgtggaaac aggcgcgcca gtacgttgaa
     5041 tggcactggc aaggcagaag taataaatga ggttgcccat catagtgagc atcaaggcgg
     5101 taagccagtc ccgacgcgcc aactgacgca gacgcacgcg tcccagccag gcaatgggca
     5161 gcgcaattaa ccctaacgcc agataacgcc ccatcgactg caacatcgcc gggtattccg
     5221 gtacgatcaa cggcccgaca aaaataagcc cccacatcaa ccctgctaac agggcgtaca
     5281 gcacgccgct aatcattact ggcatccatt ggtctgtcag aagaaagttc aggaagcaga
     5341 catgattgct cccggcatcc cgtcattatc agagaggatg cgggaggatg ccactttaat
     5401 taactagtta ttaacattaa aaagatggct tacgctttcg gctaacgttg tcgttgggcg
     5461 gccaatcaat ttgctaagcg ttttgctgtt atcaaacaga ccacctttcg atgcgccaac
     5521 gtcagaatcc gccagcatat ccgccagtcc gtcgggcagt ccgacgcttt tcagtgccgc
     5581 ggcgaaatcg gcttcgctca gattttgata ggtaacggat tttccgctct gtttggtcag
     5641 ctctgccgct aactgtgtca acgtccaggc actatcgccc gccagttcgt aaaccttgcc
     5701 ttcgtgaccg gcttcgctaa tcacgcgtgc cgcagctgcc gcataatctg cccgcgttgc
     5761 tgaggcgatt ttgccatcgc ccgccgcacc gataaatacg ccgtgttcca gtgctgccgg
     5821 ggcgctggcg aggtagtttt cggtgtacca gccgttgcgc agcagggtgt aaacgatgcc
     5881 agaatcagcc agcattttct ccgtctcgat gtgctcatcg gcgaggccga gcggggaggt
     5941 atctgcatgt agcaggctgg tataagcgat aaatttcacg ccagccgcct ttgcggcatt
     6001 aataacatta cgatgctgcg gggcacgttg acccacttcg ctggaagaga tcagcagtag
     6061 tttttccact ccctgaagtg cagatgtcag tgcggcttca tcgccgtagt cagcctgacg
     6121 cacggtaatg ccttgtgctg ccagggcctg ggcttttgcc ggattacgaa cgatagccac
     6181 tatttggctg gcaggaaccg ttttcatcaa ggattcaata acatagtgac caagttggcc
     6241 agtggcacca gtaatagcga tcatgggaag tctccatcgt tttgtcttgt gtttaaatac
     6301 gctaacacct aaacttactt ttagtaagta cgtacaaaaa ggtaagtatg aaatgagtca
     6361 ggttagcctg tcgcaacaac tgaaagaggg taatctcttt gcggaacagt gcccgtcgcg
     6421 cgaggtgttg aaacacgtca ccagccgttg gggggtattg attctggtgg cgctacgcga
     6481 aggtactcat cgctttagcg acctgcggcg caaaatcggt ggggtgagtg aaaagatgct
     6541 tgcgcagtcg ttacaggcgt tagaacagga tggttttctt aaccgtatcg cgtatccggt
     6601 ggtgccgccg catgtggaat acagcctcac gccgctgggc gagcaggtga gcgaaaaggt
     6661 tgctgcactg gcggactgga ttgagttgaa tttgcccgag gtgttggcgg tgcgggatga
     6721 acgtgcggca taactgccgg atgcgctgag cttatctggt ctacggtgca tggcttgtag
     6781 ggcggataag atgcgccagc atcgcatccg ccaataaatg ccggatgcgg ttgatgattt
     6841 ataggccgaa aaagaagtgt tttacttact caaatccacc tgataaatcg caaacccgat
     6901 atcatcggtc gcgactttat tcatcggata ttgccctttc tctttaataa acgctgcggc
     6961 tttatctgac ggggaagttt cgaaacggat atccagtttc ttatcgccag caatcggtgc
     7021 taaacgccag ttgttatctg ccgccgggtg aatttccccc gcacgtttcg actcatcagc
     7081 aatccacgct gccagcaccg agcggttctc atccggtgaa gcaaaagcga tatggctgtc
     7141 gcccgtaccg gcaaatttgc cgccgtaagc gcgatagtta ttggtggcaa cgaggaacat
     7201 ggcgttcgga tcaatcggct tgccattaaa ggtcaggttc ttaatccttt ccgcattggc
     7261 attaatcatc tggcactcgc cgtcataacg ggcaggctgg gtaacatcaa tctgataatt
     7321 cacaccatca atcacatcaa agttataggt gcggaaacca tcccagttga tgagtgattg
     7381 cggtttcgtg ctgttaggat caatctggtt aaactgtccg gcggagcact ccagccactc
     7441 tttcacctct ttaccgctgg ctttcactac aatcagcgta ttggggtaga gataaagatc
     7501 ggcggcatta cggaaggtca actggccttt ttccacctcc acatagcttg ccgggtcatt
     7561 tttgcgacca ccaactttaa acggtgcggc agctgaaagc accggcagtt ttgccagatc
     7621 cggatcgccc tgaatgtaat gttcgacata cgctttttgc gcgttgttca ccacctgcac
     7681 ggtcggatcg tcctgcacca gtgccagata gctatacata ttgtcggcag atttaccgat
     7741 tggcttgctg acgaactggc gtgtggcatc gtgatcggct ttgagtgttt ctaccagctt
     7801 gctgtcttcc gccgcgaggg attttttatt agcgatgtcg taaatcggtc gtgcttccgc
     7861 tttcgcctgc gtcacctgcc atttaccgct gtcattactg agttgtaagt cgaccacacc
     7921 aagatgatcg ccccacatgc ccggcattac cgccggaaca ccattcagcg tgcctttggt
     7981 gatatcagcc ccttcgatat cagcaaaatc tttacccggg aaaacggcgt gagcatggcc
     8041 aaacataatg gcgttaacgc ccggaatttc actgaggtaa taaactgagt tttccgccat
     8101 cactttatac ggatcggcag acagcccgga atgcgccaga acgacaacga catcggcacc
     8161 tttctcgcgc atttcaggca cgtatttgcg cacggtttcg gtaatatcat tcaccgtcac
     8221 ttttccggat aaattagctt tatcccagcc catgatttgc ggcggcacga cgccaatata
     8281 gccaatcttc agcgtctgtt tttttccgtc tttatcgacc acttcggtat ctttaattaa
     8341 atacggtgta aacattggct gtttggttct ggcgtcaatg acgttggcat ttacataagg
     8401 gaatttcgct cccgccagcg catttttcag gtaatccaga ccgtagttaa attcatgatt
     8461 gccgagtgta ccgaccgtat aatccagcgt attcagcgcc ttataaaccg gatgaacatc
     8521 acctgctttt aatcctttcg ccgacatgta atcggccagc ggactcccct gaatcaaatc
     8581 gccgttatcg accagtacgc tgtttttcac ttcattgcgg gcatcgttaa tcaggcttgc
     8641 cgtacgtacc agtccgaatt tttccgtggc ggtgtctttg taataatcga aatccatcat
     8701 gttgctatgc agatcagtgg tttccatgat acgcagatcg accgtcgctg cattcacact
     8761 ggcggcaatc agcgtggcca ggagcgttgc gctaaactta atcatcaggg acatcctttt
     8821 atcatcggga atacgaaaga aaagggagaa taaacgtctt acttatagaa cagtgaagaa
     8881 tgccacaatt ttacgctttg aaaatgatga cactatcaca gttggcgcat tcattaacga
     8941 tagggtataa gtaaaacaat aagttaacac cgctcacaga gacgaggtgg agaaatgtta
     9001 gatcaagtat gccagcttgc acggaatgca ggcgatgcca ttatgcaggt ctacgacggg
     9061 acgaaaccga tggacgtcgt cagcaaagcg gacaattctc cggtaacggc agcggatatt
     9121 gccgcacaca ccgttatcat ggacggttta cgtacgctga caccggatat tccggtcctt
     9181 tctgaagaag atcctcccgg ttgggaagtc cgtcagcact ggcagcgtta ctggctggta
     9241 gacccgctgg atggtactaa agagtttatt aaacgcaatg gcgaattcac cgttaacatt
     9301 gcgctcattg accatggcaa accgatttta ggtgtggtgt atgcgccggt aatgaacgta
     9361 atgtacagcg cggcagaagg caaggcctgg aaagaagagt gcggtgtgcg caagcagatt
     9421 caggtccgcg atgcgcgccc gccgctggtg gttatcagcc gttcccatgc agatgcggag
     9481 ctgaaagagt atctgcaaca gcttggcgaa catcagacca cgtccatcgg ctcttcgctg
     9541 aaattctgcc tggtggcgga aggacaggcg cagctgtacc cgcgcttcgg accaacgaat
     9601 atttgggaca ccgccgctgg acatgctgtg gccgcagctg ccggagcgca cgttcacgac
     9661 tggcagggta aaccgctgga ttacactccg cgcgagtcgt tcctgaatcc tgggttcaga
     9721 gtgtctattt attaattaat ttatgcagca ggtccatcac ttgctgcacc tcttcctgag
     9781 taagcgcccc gtctttggcc cattgcacgc gaccgtcttt atccagcacc actacagcgg
     9841 aacttttttc atccagctgc caggcaccgc gtgcgacgcc attgctatca acaataaact
     9901 gcgaccaggg ataaagcttt ttattactct ccagactgct gcgcacaaac atgccggaac
     9961 ccggaattgc gtcgtcggtg ttaacaatgg tggtggtctg gtaacgatcg tgcggtaact
    10021 tcgctgattt aatcgcttca atcagcgtcg cgtttttctc ttttgcagag gtgcgaccag
    10081 caatatgttg cagtactcgc acttttccca ctaactgcgc gctgttccag gttttgtagc
    10141 taaactgatc tttatcaagc accaactcgc cacgatcggt gatgccaatc ggcggcactc
    10201 gctgaccggt ttcgaactga tgtgcggaag ccatcatcgg caacagcagg caggtgagtg
    10261 ccagaatctt gcgtagggtc atggtgtttc cttcttatga tatgcaggtg atccgaccac
    10321 ttgggtctga gttttaatca tatgtgctat ttatcgaatt tcccgcaagt gtgatgccag
    10381 tttgcggtca agcgcacaaa tcatatgaaa aatgaatgct tatactgaag accgcgcttc
    10441 ggtaaaaaga taattctgaa taattgtaac ctttaggtaa aaaaagttat acgcggtgga
    10501 aacattgccc ggatagtcta tagtcactag gcattaaaat ttgcgcctca taatagttgg
    10561 gccgattgtg gcaccgcaca ggcgtaatac tcagcaggag ataacaatga aaattttcca
    10621 acggtacaac ccacttcagg tggcgaagta cgtaaagatc ctgttccgtg gacggttata
    10681 catcaaggac gttggcgctt ttgaatttga taagggtaag attcttatcc caaaagtgaa
    10741 ggataagctg catttgtcag tgatgtccga agttaaccgt caggttatgc gtctgcaaac
    10801 agagatggct taaccaaagt gctatgcagt aataagacgg ctcctgattc aggagccgtt
    10861 gatgtttctg ggggttacgc gacgctttct tctttatctt tagcgtcagg cagttttggc
    10921 gaaagggcgg tggccttgct gtcgatccgg gtcaccagca gctggtcgat gcggtagtta
    10981 tcgatatcca ccacttcaaa tttgtagccg gcgaatttca ccgaatcggt gcgtttcggg
    11041 atcttacgca acataaacat cataaagccg ccgatggttt cgtagttgcc cgactgcggg
    11101 aattcgtcaa tatccagcac gcgcatgacg tcgtcaatcg gggtgccgcc gtcaatcagc
    11161 catgagttct catcacgggc gacaatctgc tcttccagcc cctgaccgac cagatcgccc
    11221 atcagcgtgg tcatcacgtc gttgagggtg atgatcccca ccaccagcgc gtactcgttc
    11281 atgatcaccg cgaagtcttc acctgcggtt ttaaaacttt ccaacgcctc tgaaagggtt
    11341 aacgtatccg gcacaatcag cgtgttgcga atttgtacgc cgctgttcag tgccaggctt
    11401 tggttagcca gcacgcggtt cagcaggtct ttagaatcga catagccgat gatgtggtca
    11461 atatcttcat tacagacgag gaacttagag tgcggatgtt ccgccacctt atttttcaga
    11521 ctttgctcat cttcgtggag atcaaaccaa atcacgtttt cacgcggtgt cattgaagac
    11581 ggaacggtac gggattccag ctcaaagacg ttttcaatca gctcgtgttc ctgtttacgt
    11641 aacacgcccg ccagcgcacc ggcttccact accgcgtaga tgtcatcaga agtgatgtca
    11701 tctttacgta ccattggcag tttgaaaata cggaagatta tgttcgccag gccgttgaag
    11761 aaccacacca gcggggtgca aacgtacagg cagaagcgca tcgggttgat gatacgcaaa
    11821 gccaccgctt ctggcgcaat cataccgatg cgtttcgggg ttaaatccgc aaacaggata
    11881 aacatgccag tcactaacga gaaagagaga ataaagctca gttgctcaga gagttctgcc
    11941 gacatatagc gggagaacag gctgtgaaaa gctggagaaa atgccgcatc accgacgata
    12001 ccgccgagaa ttgccactgc gttcagaccg atttggacca cggtaaagaa catgccggga
    12061 ttttcctgca tattcagaac gcgttgggca tttatattgc cttcatcagc cagcagttta
    12121 agtttgattt tgcgtgaggc ggcaagcgag atctcggaca tcgagaagaa cgcacttaca
    12181 gcgatcaagc agagtatgac taaaatactg tttaacatat cttatccgac ctttcaggcc
    12241 agatcctcgg aaggggaagt tgattaattt gtgtgaaata cacattgaaa gccgattcga
    12301 agaaagtgaa ccggcatttt cagtggctag tatagcgtaa ggtactgtaa agccgccaga
    12361 gggttaaaat tcagacagct gaaaaatgca aaactgccga tgcgctatgc cgatcaggcc
    12421 tacgcagtcc ttgcaatata ttgaatttgg acgattttgt aggcctggca aggcgtttac
    12481 gccgcatccg gcataaacaa aacgcacttt atcaacaatc tacatttata cccgtaacgc
    12541 tatgcttccg gtggcagaca gacgccaatt ccgccaattc cacagtaacc atacgggttt
    12601 ttatgcagat attgctggtg gtcatcttcg gcataataaa acggtgtggc gttagcgatt
    12661 tccgtggtga tgtgacgatc atcatcggcg gcaagcatcg ccgcctgaaa acgttccaga
    12721 ctggcgcgag ctgcggcatc ctgttctggg gtcagcggat aaatcgctga acgatactgc
    12781 gtgccgtggt cattgccctg acgcattccc tgggcgggat cgtgattctc ccaaaatacc
    12841 tgtagcaact gctcatagct gatgacggaa ggatcgtaaa caatgcgtac cgcttcggca
    12901 tgacccgtat caccggagca cacttcccga taagtcggat ttggcgtata gcctccggta
    12961 tagcctgcgg cggtgctgta aacgccgggt aactgccaga acagacgctc cacaccccag
    13021 aaacaaccca tcgcaaaaat ggcaatctcc attccgtcag gtacattggt cattgagtga
    13081 ccgttgaccg catgcagcgt ggctacgggc atcggggtgt tacgtccagg cagggcatcg
    13141 gcgggggaaa ccagatgctt tttatcaaat aaactcatgg tgtcgctctc ccgaaaatcg
    13201 gtcattgggg ttaaggttgt aacaagaggc gtatttgcac acaataacca ccgtgaatag
    13261 gtctaaagta aaacataaga aatatttggg gtttagtctg ctttttaatc catattactg
    13321 gatttttgtt aagccgttta acggcgttcc aggggcagga aaaaaggata ttcaggagaa
    13381 aatgtgcgct atatccgaca gttatgctgt gtaagcttac tctgcttaag cggatctgcc
    13441 gtcgccgcga acgtccgtct acaggtcgag gggttatcgg gacagctgga aaagaacgtt
    13501 cgtgcgcagc tttctacgat tgaaagtgat gaagtgacgc cagaccgtcg ctttcgcgca
    13561 cgcgtcgatg atgccatccg tgaaggtctg aaagcgctgg gttattacca gccgaccatt
    13621 gaatttgatc tccgtccacc gccaaagaaa gggcggcagg tattgatcgc caaagtcacg
    13681 ccaggtgtgc cggtgttaat tggcggcacc gatgtggtat tgcgcggcgg cgcgcggacc
    13741 gataaagact atttgaaatt gctcgatact cgcccggcta ttggcacggt gctgaaccag
    13801 ggcgattatg aaaatttcaa aaagtcctta accagcattg cgttgcgtaa aggttatttc
    13861 gatagcgaat ttaccaaagc gcagctgggc attgcgctcg gcctgcataa agccttctgg
    13921 gatattgatt ataacagtgg cgaacgttac cgctttgggc atgtgacctt tgaaggatca
    13981 caaatccgcg atgaatacct gcaaaatctg gtgccgttta aagagggcga tgagtacgaa
    14041 tcgaaagatc tggcggaact gaaccgccga ctttctgcta ccggctggtt taactcggtg
    14101 gtggtggctc cacaatttga taaagcgcgc gaaacgaaag tattaccctt gacgggcgtg
    14161 gtttcgccgc gaactgaaaa caccatcgaa accggggtcg gttactctac ggacgtggga
    14221 ccgcgcgtga aagcgacgtg gaaaaagccg tggatgaact cttatggtca cagtctgacc
    14281 accagtacca gtatttccgc gccggaacag accctcgact tcagctataa aatgccgctg
    14341 ctgaagaatc cactggaaca atattatttg gtgcagggcg gttttaagcg cactgacctg
    14401 aacgataccg aatctgactc cactacgctg gtggcttctc gctactggga tctctccagc
    14461 ggctggcagc gtgccattaa cctgcgctgg agcctcgacc actttaccca gggtgaaatt
    14521 accaacacca cgatgctgtt ttatcctggg gtgatgatta gccgcacgcg ttctcgtggt
    14581 ggcctgatgc caacctgggg cgactcgcaa cgctactcta tcgactactc caacacggcc
    14641 tggggctcag atgtcgattt ctccgttttc caggcgcaga acgtctggat ccgcacactg
    14701 tacgatcgcc atcgttttgt gacacgcggc acgctgggct ggattgaaac gggtgatttc
    14761 gacaaagtac cgccggatct gcgtttcttc gccgggggcg atcgcagtat tcgcggctac
    14821 aaatacaaat ctatcgcccc gaaatacgcc aacggtgacc tgaaaggggc ctcgaagctg
    14881 ataaccggat cgctggaata ccagtacaac gtgaccggaa aatggtgggg cgcggtgttt
    14941 gtcgatagtg gcgaagcggt aagcgatatt cgccgcagcg actttaaaac cggtaccggg
    15001 gtcggcgtac gctgggaatc gccggtcggg ccaatcaaac tcgattttgc cgtaccggtc
    15061 gcggataaag acgagcacgg gttacagttt tacatcggtc tggggccaga attatgagtt
    15121 tatggaaaaa aatcagcctc ggcgtggtta tcgttatctt actgttgctg ggatcggtgg
    15181 cgtttctggt gggcaccacc agcggcctgc atcttgtatt taaagcggcg gatcgctggg
    15241 tgccaggact ggatattggc aaggtcaccg gcggctggcg cgatctcacc ttgtctgacg
    15301 ttcgttatga gcagccaggc gtggcggtaa aagcgggcaa tctgcatctg gcggtcgggc
    15361 ttgagtgcct gtggaacagt agcgtttgta ttaatgacct ggcgctgaaa gacattcagg
    15421 tcaacatcga cagtaaaaaa atgcctcctt ctgaacaggt tgaagaagag gaagatagcg
    15481 gtccgctgga tctctccacg ccgtatccca tcaccctgac acgggtggcg ctggacaacg
    15541 tcaacatcaa gattgatgac accacggtgt
//
LOCUS       DAEVZA010000113        15419 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEVZA010000113 DAEVZA010000000
VERSION     DAEVZA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.113"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(168..1079)
                     /gene="gsiD"
                     /locus_tag="LAF92_003717"
     CDS             complement(168..1079)
                     /gene="gsiD"
                     /locus_tag="LAF92_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBI9792653.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1082..2002)
                     /gene="gsiC"
                     /locus_tag="LAF92_003718"
     CDS             complement(1082..2002)
                     /gene="gsiC"
                     /locus_tag="LAF92_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBI9792654.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2020..3558)
                     /gene="gsiB"
                     /locus_tag="LAF92_003719"
     CDS             complement(2020..3558)
                     /gene="gsiB"
                     /locus_tag="LAF92_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBI9792655.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINPPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3578..5449)
                     /gene="gsiA"
                     /locus_tag="LAF92_003720"
     CDS             complement(3578..5449)
                     /gene="gsiA"
                     /locus_tag="LAF92_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBI9792656.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPFISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5436..6401)
                     /gene="iaaA"
                     /locus_tag="LAF92_003721"
     CDS             complement(5436..6401)
                     /gene="iaaA"
                     /locus_tag="LAF92_003721"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBI9792657.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6605..7840
                     /gene="moeA"
                     /locus_tag="LAF92_003722"
     CDS             6605..7840
                     /gene="moeA"
                     /locus_tag="LAF92_003722"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBI9792658.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7840..8589
                     /gene="moeB"
                     /locus_tag="LAF92_003723"
     CDS             7840..8589
                     /gene="moeB"
                     /locus_tag="LAF92_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBI9792659.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARYALTRINPHIA
                     ITPVNALLDDAELAVLIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8768..9430)
                     /gene="fsa"
                     /locus_tag="LAF92_003724"
     CDS             complement(8768..9430)
                     /gene="fsa"
                     /locus_tag="LAF92_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBI9792660.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9561..10460
                     /locus_tag="LAF92_003725"
     CDS             9561..10460
                     /locus_tag="LAF92_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBI9792661.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10466..12898
                     /locus_tag="LAF92_003726"
     CDS             10466..12898
                     /locus_tag="LAF92_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBI9792662.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHELCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13044..13859
                     /gene="ybiV"
                     /locus_tag="LAF92_003727"
     CDS             13044..13859
                     /gene="ybiV"
                     /locus_tag="LAF92_003727"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBI9792663.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGNQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNQPDEQIPL
                     VIDKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQALLDNTSPFNS"
     gene            14011..15276
                     /locus_tag="LAF92_003728"
     CDS             14011..15276
                     /locus_tag="LAF92_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBI9792664.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3721 a         4269 c         3941 g         3488 t
ORIGIN      
        1 ttaacaaaat aactatctga acgctgtgcc gctgcaaaaa tagtcccttg ctgcggcttt
       61 ttaaccaaat tacccgattc tacaaaatca gcagaaagtt acaacacttg cttagggcaa
      121 taattgcctg tgaggtgatt accctttcaa gcaatattca aacgtaacta tcctttaatt
      181 ttcggatcca gcgcatcgcg taaaccatcg cccaacaaat tgaacgccag tacggtcaga
      241 aaaatagcca gggccggaaa aacagcgaca tgcggcgcga taaccatatc cgctcgtgcc
      301 tcattgagca ttgctcccca ctctggtgtc ggcggctgcg caccgaggcc gagaaatgac
      361 aggctggcgg cagagataat cgaggtgcca atgcgcatgg tgaaaaacac cacgatagac
      421 gagacggtcc caggcaagat atgacgcaat aaaatggtca tatcgctggc accaatactg
      481 cgtgctgact caataaaggt ttgctgtttc aacaccagcg tgttgccgcg caccaggcgg
      541 gcaaacgcgg ggatggaaaa aatggcgact gcgataatca cgttagcaat gccgcttccc
      601 aacaccgcaa caacagcgat cgccagtaaa atacccggga aggcaaacag cacatcgcaa
      661 atgcgcatga tcagccgatc ccaccagcct tcataatatc cagccagcaa gcccagcaac
      721 gtccctatcg ccgcaccgat aaacacggca aacacgcccg ccgccagcga gatttgcgca
      781 ccaaccagga cacggctgaa aatgtcgcgc cccagtgaat cgacgccaaa ccagtgctgc
      841 aaagaaggtc cgttattcag attgtcataa tcaaaataat tttcggcgtc atagggagcg
      901 atccagcgtg caaaaatggc caccacaatc aataaaataa cgaataatgc ggcggtcatc
      961 gccatatgct gacggcgaaa tcgtcgccag aattcatgcc acggtgtacg cacctggtca
     1021 ggtttgacca gtggcatggc gtttaacacc gcctgacgtc gccagttaaa tagtcgcatc
     1081 cttacttgta cctgatagcc gggttaatgg cggcgtaaag cacatccacc actaagttga
     1141 taagaataaa ttccagcgag aaaagcagaa tttccgcctg aatcaccggg taatcgcgca
     1201 tttctacgga gtcaacgagt aagcgtccaa gtccgggcca gttgaaaact ttctccacca
     1261 cgatagaacc accgagcaaa aagccaaact gtaagcccat catggtcact accgggatca
     1321 tcgcgttacg tagcccgtgt ttgaggacaa cccaggtttc gctcacccct ttcgccctcg
     1381 cggtacgcat ataatcttcg cttaaaacat cgacaaacga cgcgcgggta aagcgcgcca
     1441 tcacggctgc cactgccgcg ccgagcgtca gggagggtaa aatgtagtgc tgccaactgt
     1501 ctgctcccac ggtaggcagc cagcccagtt caacggagaa tacctgaatt aaaagcatcc
     1561 ccagcgcaaa tgccggaaac gagatccccg acaccgcaat ggtcatactc aatcgatccg
     1621 gccaacgatt acgccagacg gcggcgataa ttcccgccgc cataccaaat atcaccgccc
     1681 agaccatact ggttatggtc agccacagcg ttggcataaa gcggctggca atctcatcgg
     1741 caaccggacg acgcgacacc atcgacaggc caaaatcccc ctgcacagca ttgctgatat
     1801 agtgccagaa ctggtgatac agcggctgat ccaaccccag ctgctgacgc accagttcta
     1861 taacctgcgc atcagcttcg ggcccggcaa tcaatcgcgc cggatcgccg ggcagcatat
     1921 gcacaaataa aaacaccagc accgagacga taaacagcgt cggaatcaac cccagtaatc
     1981 gtttgataac gtaattaagc attccactcc ctgcgttgct tattgcaaat ccgcgtcttc
     2041 aaagctgaag ccggtgtctg gcatgatcca aaaaccggtc aggtttttac tgtgtgccga
     2101 caccagtttt tctaccacca gcgggatcca cggcgactct tgccagatga tatcctgcgc
     2161 cgccttatac aagcgggtct tttccgccgg atcattagtt ttcagtgcct gagccaggaa
     2221 gtcatccacc tgtttattgc tgtaaaacgc ggtattaaac agcgtcggtg gccagttttg
     2281 cgaggcaaac aacggcgata gtgcccagtc ggcttcgccg gttgaagccg accagccagt
     2341 gtagaacatc cgcacgccgc tctctttttg ccctttacct tcaacttctg ccgcccgctg
     2401 tccggcatcc atcgcagtca cctgggcttt aatcccgacc tgcgctaact gctgctgggt
     2461 aaattgcagc actttctgcg cggtgctgtg gttatgtgac gaccacagcg tggtactgaa
     2521 accgttggga tatcccgcct ctttcagtaa ttcgcgcgct ttcactggat cgtaaggcca
     2581 cggtttataa ctttgcgcgt aggcgatact tggcggtacc acaccagtag ctggcgttgc
     2641 atagcccgca aaggcaactt tcaccagcgc cggagggtta atggcgtaat tcagcgcctc
     2701 acggaccttc gggttatcga acggcttttg cgtcacgttc atactgatat aacgctgcat
     2761 aattgacgga ctggccatca actcgatatt tttgtttttc tccagcagtg tggcctgctc
     2821 gtagggaatg gggaaagcaa actgcgcttc accggtttgc agcattgccg cgcgggtgtt
     2881 gttatccgcc accggacgcc aggttatgct gtccagtttg ggcaatcctg gctgccagta
     2941 acccgcgaat tttttcacct tcacaaaatc ggtctgattc caggtatcca gttcatacgg
     3001 tccggttccc accggatgaa aaccaatctc cttgccatat ttttccagcg ctgccggtga
     3061 aatcatcgcg gtcgccggat gggcaagaat attaataaac gctgagaacg gctgtttgag
     3121 agtaatcttt actgttgttg gatcgatcgc ctccgttttg gcaatattct tatacaggtt
     3181 atagcgttta agatgattcg ccggatcgct ggcccggtcc agattcgctt ttaccgccgc
     3241 tgcgttgaaa tcggtgccat cctggaattt aattccttcc cgcaatttca cggtgtatgt
     3301 aaggccgtca tcggaaacgg tataactctc cgccagcacg tttttcagtt tcatctcttt
     3361 atccagacca aacagcccct ggtaaaacga tttcgctacg gcctgagata acgtgtcatt
     3421 tgcgtcatac ggatcgagcg tggtgaagtt cgatcctacc gccaccacca catctttggc
     3481 agcgaatgca caagatgcca tcaacgctgt cgcaatgccc aacgccacta acccactacg
     3541 gtgtacagct cttgccattt tattctccgc ctgaatgtta tctacgcatg aatgcgtatt
     3601 ctgattgtgg ttgtgcgacg taatgccccg gcccgacgca ttgcaacgag acggctgcca
     3661 cctcttcgcc acgcagatga atattgctgg gaagatcgtc cgacagcagt acacgctgcg
     3721 gtcgttgtcg ggacggttca gcgaccggaa ctgccgccag taatttacgc gtataaggat
     3781 gctgcgggtt ttcgaagacc gcgcgccgtg gaccaatttc aacaatttgt ccgagataca
     3841 tcaccgccac acgatgacta atccgctcta ccacggccat atcgtgggag ataaacagat
     3901 acgcaatgcc gaaatcacgc tggagatcga gcaacaagtt gataatctgc ccgcgaatag
     3961 aaacatccag cgccgagacg gcttcgtcgg caatgatcac tttcggattc aatgccaacg
     4021 cgcgagcaat gcagatgcgc tggcgctgac cgccggaaaa ctcatgcggg taacgccagg
     4081 catgttcagg taacaggccc acgcgctcca gcaaccacgc aacgcgtgca gccgcttctt
     4141 tacctggcaa taaaccgtgt acacgcagcg gttcgataat cgaatcaccg atggtctgac
     4201 gtgggtccag cgaagcgtaa gggtcctgaa aaataaactg aatatcccgg cgcaatgcct
     4261 gaagtttgcc gggtgacaag gtatcgattc tctgaccgtt aaagataatt tcgccgccct
     4321 gcgattcgac caggcgcagc aacgcccgcc cggtagtgga tttaccgctg ccagactcgc
     4381 ccaccagcga tagcgtttcg ccaggccaga gatcaaaact gactttctca acggcatgca
     4441 cttcccgcgt tacgcgattc aacaaaccgc tgcgcaaagg gaaacgggtg acaagattac
     4501 gcactcgtaa aacaggttcg ccatccacca ccgttttctg ctcgatgggg ggggcctgtt
     4561 tcgctggatg ttcaagcgat ataaacggga aacgtcgggg ataatctaac cctttcatcg
     4621 caccaagttg cggaacagca gctaacagcg cacgggtgta aggatgttgc ggtgcatgaa
     4681 aaatctgttc gacggtaccc gtttccaccg cctcgccctg atacatcacc agtacccgat
     4741 cggcaatctc tgccaccacg cccatatcgt gagtgataaa gataacgccc atcgacatct
     4801 ctttttgcaa tactttgatt aattgcagga tctgcgcctg aatagtgaca tccagcgcgg
     4861 tggttggctc atcggcaatc agcaccgccg ggcggcatga cagcgccatc gcaatcatca
     4921 ctcgctggcg catcccgcca gagagttgat gcggataacg tgaaagaatg gtttgtgcct
     4981 caggaatgcg tacctgatcc agcatccgct tcgcctcgac catcgcttct tcacgactgg
     5041 cgttctgatg cagacgaatt gattcggcaa tctgttcacc cacagtaaat accgggttca
     5101 gcgatgtcat cggctcctga aatatcatcg ccatatccgc accgcgaaca tggcgcattt
     5161 gtgcagcgct ctgctcgcta agttcaatca cttcgcgact gcgccgctgc aacagcattt
     5221 tatcgcactg tactaaaccg cccgcctgtt ccaacaggcg catcaatgcc aacgcagtca
     5281 ctgacttacc ggagccggat tcgccaacaa ttgccagcgt ctcaccgcgt tgcagactaa
     5341 aagagagatt gcggaccgca gctattttct gctggtcctg cataaaggca atattcagat
     5401 tttcaaccgc cagcacatta ccggcatcaa gttcatcact gtgtggcaac ggtgtcccct
     5461 ttttcacggt agataccggt ggttggcgta tcgcctgcgt agccccaggc gcgatacatt
     5521 ccttcggtgt taaacggtag cgcgacattc ccttcatggt cgatagcgat taagccaccg
     5581 ctaccgccaa gcgcagggag tttttccatt actacccgct cgcaggcttc cgcgagactt
     5641 aatccgccgt aatccattaa cgcggcgatg tcatatgccg ccagcgcgcg gatgaagact
     5701 tcgcccgtgc cggtacaaga aaccgccaca ctggcgttat tggcgtagca tccggcaccc
     5761 actaagggac tatcgccaac tcgtccgggt aatttattgg tcattccgcc tgtggacgtg
     5821 gctgccgcca aattgccgtc taaatccaac gccacggccc ccacggtgcc cattttctgt
     5881 ttttcatcca gtggcgcacc gctatggtcg aggacggttg ccccttcctt gcgcgctgcc
     5941 agtagttgtt cataacgcaa aggcgtggag aaaatctccg gcgagacgcg ctccatgcca
     6001 cgagcaaacg caaaattttc tgccccttcg ccaatcatca tcacatgcgg gctttgctcc
     6061 atcaccagcc gggcggcaag aaccggatta cgcagatgac taacgcccgc caccgcaccg
     6121 gctttcaggg tgttaccatc catcacacag gcgtccagtt catgggtttc atcacgcgta
     6181 aagacagcgc caattccggc gttaaacagt ggacactctt ccagcagacg caccgcttcc
     6241 gtcaccacat ccagcgcact ttcgcccgct tccagcattt tctgcccggt ttcaacaatg
     6301 gcagacaacg cctcaatgta gcgtaattcc tgttgcagac tcatctgcgc gcggctaatt
     6361 gcacctgcgc caccatgaat tgcaatgact gctttgccca tagtattcgt ccattaaggg
     6421 tggctcgcca cttttgctat aaatatcaga atttttatga attactcatt caatttttga
     6481 atatagaaag atggatatgg catgtaaagg caggagccaa cgctacgccc gaggtggcat
     6541 acagtctgtg gcgtttttct gacataatag gcaaattcga ttttgcctcc gcaggagtgt
     6601 tttcatggaa tttaccaccg gattgatgtc gctcgacacc gcgcttaatg agatgctttc
     6661 tcgcgtcacc ccactgaccg cccaggaaac gctgccactg gtacagtgtt ttggtcgtat
     6721 tctggcgagc gatgtcgttt cgccgcttga tgttccgggg tttgataact ccgcaatgga
     6781 cggctacgcg gtgcgtttag ccgatattgc ctccaggcaa ccgctgcccg ttgccggtaa
     6841 atcctttgcc ggtcagccgt accatggtga atggcctgcg ggtacctgca ttcgtattat
     6901 gaccggtgcg ccggtgccgg aaggctgcga agcggtggtg atgcaggagc agactgaaca
     6961 aacggacaat ggcgtgcgtt ttactgctga agtgcgtagc gggcaaaata ttcgccgtcg
     7021 cggtgaagat atctctgcag gtgcggttgt tttcccggcg ggaactcgcc tgactaccgc
     7081 tgaactgcca gtgattgctt cactggggat tgccgaagtt ccggtgattc gtaaagtgcg
     7141 tgtagcgctt ttttctaccg gtgatgaact ccagttgccc ggtcagccac tgggcgatgg
     7201 ccaaatctac gataccaacc gtctcgccgt acacctgatg ttagaacagt tgggatgcga
     7261 ggtaatcaac ttagggatta tccgcgacga tccacatgcc ctgcgcgccg catttattga
     7321 agccgacagc caggcggatg tggtgatcag ttccggcggt gtttcagtgg gtgaagcgga
     7381 ttacaccaaa accattcttg aagagctggg ggagatcgcc ttctggaagc tggcgattaa
     7441 accgggtaaa ccgttcgcgt tcggtaaact ttctaatagc tggttctgcg gcctgccggg
     7501 caacccggtt tcagcgacgc tgaccttcta tcaactggta cagcctttgc tggcaaaact
     7561 aagcggcaac accgccagcg gcctgcccgc gcgccagcgc gtacgcacag cgtcccgcct
     7621 gaaaaaaacg ccaggacgtc ttgatttcca gcgcggcgtg ctgcaacgca acgccgacgg
     7681 tgaacttgaa gtgacgacca ccggacatca gggttcacat atatttagct cctttagtct
     7741 cggcaactgc tttatcgtgc tggaacgcga tcgcggcaat gtggacgtgg gcgaatgggt
     7801 ggaagtagaa ccgtttaacg cgttgttcgg aggcctgtaa tggcggaact cagcgatcag
     7861 gagatgctgc gctacaaccg gcaaatcatt ctgcgcggtt ttgattttga cggtcaggag
     7921 gcgctaaaag attctcgtgt gctggtagtc ggcctcggtg gactcggctg cgcagcttcg
     7981 cagtatctgg caagcgccgg tgtcggcaac ctgacgctgc tcgacttcga cacggtttcg
     8041 ctctcgaatc tgcaacgcca gacactgcac agtgatgcca cggtcgggca accgaaggtg
     8101 gaatccgccc gttacgccct gacgcggatc aacccacata tcgcgattac gccagtcaat
     8161 gcactgctag atgacgcaga acttgcagta ttgattgctg aacacgatct ggtgctcgac
     8221 tgtacggata acgttgcggt acgtaatcaa ctgaacgcag gctgttttgc cgcgaaggtg
     8281 ccgctggttt ccggcgcggc aattcgtatg gaagggcaaa tcacggtctt cacttatcag
     8341 gacggcgaac cgtgctatcg ctgccttagc cgtttgtttg gcgagaacgc attaacctgc
     8401 gtagaagcag gcgtgatggc accgttgatc ggcgtaattg gttcgttgca ggcaatggaa
     8461 gcgatcaaac tgctggcagg ttatggcaaa ccggccagcg ggaaaatcgt catgtacgat
     8521 gcgatgacct gtcagtttcg cgaaatgaaa ctgatgcgca atccggggtg tgaggtgtgc
     8581 gggcagtaat tgctacctcc aggcctgata agacgcgaca agcgtcgcat caggcatttg
     8641 ctccagacgc cagatgcggc gtgaacgcct tatctggcct acggttcggg cacagatttc
     8701 caagcgcggc aggcatcggt gcactccgcc acgtcatcag caatttatga ggatgcagaa
     8761 cgtccggtta aatcgacgtt ctgccaaacg ctcccagcca gtcctgttca aacttcgcca
     8821 cagcggcatc aaccgccgga tagctaatca tctgttgtgc cacatccagt ggcagcgtaa
     8881 ttgattcaca tcctgccagt aagcagtcca gcgcctgacg cggggttttg aaactcgctg
     8941 ccagcacttt cgcctgcggc gcatgcattt tcaataactg gtgtaagtcg gtcacagtct
     9001 gaatgccgct accgccctga gcatcaatac gattaacgta aggcgcaaca tattccgcac
     9061 ctgccagcgc cgacagcagc ccttgtgctg cgccatatac cgcggttccc agcgttggaa
     9121 tcccttccgc ttttaacatc ttaatagctg ccagcccctc ggcggtcacc ggaactttca
     9181 ccacgatatc cgcaataata gaacgcaatt tacgtgcgtc attcaccatc ccttcagcag
     9241 tggtagccat tacctgggca aacagacgcc cctgaccgcc catcgcttca tgaagttgcg
     9301 gaagcacaac atccagcggt tttttacccg cggcgataat gcttgggtta gtggtcacac
     9361 ccgccagcgg aaaaatacgt gacagcgcct tcaccgcaac aacgtctgaa gtatccagat
     9421 acagttccat aaccatcctc aaaatgtact ttttgtctga atgtagacta tcacgggaaa
     9481 actctctgaa cagttgacgc acatcaatct aactttcatt cgaaagtatt ttaatgtttg
     9541 aacgaaacag agaggcaact atgattttca atattcagcg ctactcgacc catgatggcc
     9601 ccggtatccg cacggtcgta tttcttaaag gctgttcgct gggctgccgc tggtgtcaga
     9661 acccggaaag ccgcgcccgc acgcaggatc tgctgtatga cgcacgactg tgtctggaag
     9721 gctgcgagct gtgcgctaag gccgcgccgg aagtgattga gcgcgcgctg aatggtttgc
     9781 ttattcatcg ggaaaagtta accccggagc atctgacggc gttaaccgac tgctgtccga
     9841 cacaggcatt aaccgtgtgt ggtgaagtga aaagcgttga ggagatcatg acgaccgttc
     9901 tgcgcgataa accgttttat gatcgcagcg gcggcggttt aacgctttcg ggtggtgagc
     9961 cctttatgca gccggaaatg gcgatggcgc tactgcaagc cagccacgag gcaggcattc
    10021 atactgcggt agaaacctgt ctgcatgtgc cgtggaaata tatcgcccct tctctgccct
    10081 atatcgatct gtttcttgcc gatttaaaac acgttgccga cgcgccgttt aaacagtgga
    10141 ccgacggtaa cgccgccaga gtgctggata acctgaaaaa actcgccgca gcgggcaaaa
    10201 aaatcattat ccgcgtgccg ctgattcagg gctttaatgc cgacgaaacc tctgtaaaag
    10261 ccattaccga ttttgccgcc gacgagctgc acgttggcga aattcatttt ctgccctacc
    10321 acacgctggg catcaacaaa tatcacttac ttaatctgcc ctatgacgcc ccggaaaaac
    10381 cgcttgatgc gccagaactg ctcgactttg cccagcagta tgcctgccag aaagggttaa
    10441 ccgcgacctt acgaggataa caaccatgac cacactgaaa ctggacacgc tcagcgaccg
    10501 cattaaagcg cacaaaaatg cgctggtgca tattgtgaaa ccgccagtct gtaccgagcg
    10561 cgcgcagcac tataccgaga tgtatcaaca acatctcgat aagccgatcc cggtacgtcg
    10621 cgcgctggcg ctggcacatc acctggcgaa tcgcactatc tggatcaagc acgatgagtt
    10681 gatcatcggc aaccaggcaa gcgaagttcg cgccgcgccg atcttcccgg aatatactgt
    10741 ctcctggatc gaaaaagaga ttgatgatct ggcggatcgt cccggtgctg gttttgcggt
    10801 gagcgaagag aacaaacgcg ttctgcatga attgtgcccg tggtggcgcg gtcagaccgt
    10861 acaggatcgc tgctacggca tgtttaccga tgagcaaaaa ggtctgctgg cgaccggcat
    10921 cattaaagcg gaaggcaata tgacctccgg cgatgcgcac ctggcggtga atttcccgct
    10981 gctgctggaa aaaggtcttg atggtctgcg cgagaaagta gcggaacgtc gctcgcgcat
    11041 caacctgacg gtgctggaag atttacacgg tgagcaattc ctgaaagcga ttgatatcgt
    11101 gctggtggca gtcagtgaac acattgaacg tttcgctgcc ctggcgcgtg aaatggccgc
    11161 gaccgaaacc cgcgaaagcc gtcgcgatga actgctgacg atagcagaaa actgcgatct
    11221 tatcgcccac cagccgccgc agactttctg gcaggcgctg caactgtgtt acttcatcca
    11281 gttgattttg cagatcgaat ctaacggtca ctcggtatcg tttggtcgta tggaccagta
    11341 tctctaccca tactatcgcc gcgacgttga actcaaccag acactggatc gcgaacacgc
    11401 catcgaattg ctgcatagct gctggctgaa actgctggaa gtgaacaaga tccgctccgg
    11461 ctcgcactcg aaagcctctg cgggaagtcc gctgtatcag aacgtcacca ttggcgggca
    11521 aaatctggtt gatggtcaac caatggacgc ggtgaatccc ctctcttacg cgatccttga
    11581 atcctgcggt cgcctgcgtt ccactcagcc taacctcagc gtgcgttacc atgcaggaat
    11641 gagtaacgat ttcctcgacg cctgcgtaca ggtgatccgc tgcggcttcg ggatgccggc
    11701 gttcaacaac gacgaaatcg tgatcccgga atttattaaa ctcggtattg aaccgcagga
    11761 cgcttacgac tacgcagcga ttggttgtat cgaaaccgcc gtcggtggca aatggggcta
    11821 tcgctgtacc ggcatgagct ttatcaactt cgcccgcgtg atgctggcgg cgctggaagg
    11881 cgggcgtgat gccaccagcg gcaaagtgtt cctgccacaa gaaaaagcgt tgtcggcagg
    11941 taactttaac aatttcgatg aagtaatgga cgcgtgggat acgcaaatcc gttactacac
    12001 ccgcaaatca atcgaaatcg aatatgtcgt cgacaccatg ctggaagaga acgtgcacga
    12061 tattctctgc tcggcgctgg tggatgactg tattgagcga gcgaaaagta tcaagcaagg
    12121 cggcgcgaaa tatgactggg tttctggcct gcaggttggc attgccaacc tcggcaacag
    12181 cctggcggca gtgaagaaac tggtatttga acagggcgcg attggtcagc aacagctggc
    12241 tgccgcactg gcggatgact tcgatggcct gactcatgag caactgcgtc agcggctgat
    12301 taacggtgcg ccgaagtacg gcaacgacga tgataccgtc gatacgctgc tggctcgcgc
    12361 ttatcagacc tatatcgacg aactgaaaca gtaccataac ccacgctacg gtcgtggtcc
    12421 ggttggcggc aactattacg cgggtacgtc gtctatctcc gctaacgtac cgtttggcgc
    12481 gcagacgatg gcaacgccgg acggacgtaa agcgcatacc ccgctggcag aaggcgcaag
    12541 cccggcttcc ggtactgatc atcttggccc tactgcggtc attggctcgg tgggtaaact
    12601 gcctacggca gcgattctcg gcggggtgtt gctcaaccag aaactgaatc cagcaacact
    12661 ggagaacgaa tctgacaagc agaaactgat gatcctgctg cgcaccttct ttgaagtgca
    12721 taaaggctgg catattcagt acaacatcgt ttcccgcgaa acgctgctgg aagcgaaaaa
    12781 acatccggat cagtaccgcg atctggtagt gcgtgtcgcg ggctattccg ccttcttcac
    12841 cgcgctctct ccagacgcac aggacgatat catcgcccgt actgaacata tgctgtaatc
    12901 ttctcagccc ggcggcgtcg tcgccgggct aaatcattca catcatcaat ttcatcctta
    12961 ctttcattcg aaatataatt tgtgctctgc gtcacattat tattacactg ttacggtttc
    13021 attttcgagc caggagcaca cctatgagcg taaaagttat cgtcacagac atggacggta
    13081 cttttcttaa cgacgccaaa acgtacaacc aaccacgttt tatggcgcaa tatcaggaac
    13141 tgaaaaagcg cggcattaag ttcgttgtcg ccagcggtaa tcagtattac cagcttattt
    13201 cgttctttcc tgagctaaag gatgagatct cttttgtcgc ggaaaacggc gcactggttt
    13261 acgaacatgg caatcaactg ttccacggcg aactgacccg acatgaatcg cggattgtta
    13321 ttggcgagtt gctaaaagat aagcaactca attttgtcgc ctgcggtctg caaagtgcat
    13381 atgtcagcga aaacgccccc gaagcattcg tcgcactgat ggcaaaacac taccatcgcc
    13441 tgaaacctgt aaaagattat caggagattg acgacgtact gttcaaattt tcgctcaacc
    13501 agccggatga acaaatcccg ttagtgatcg acaaactgca cgtagcgctg gatggcatta
    13561 tgaaacccgt taccagtggt tttggcttta tcgacctgat tattcccggt ctacataaag
    13621 caaacggtat ttcgcggtta ctgaaacgct gggatctgtc accgcaaaat gtggtagcga
    13681 ttggcgacag cggtaacgat gcggagatgc tgaaaatggc gcgttattcc tttgcgatgg
    13741 gcaatgctgc ggaaaacatt aaacaaatcg cccgttacgc taccgatgat aataatcatg
    13801 aaggcgcgct gaatgtgatt caggcgctgc tggataacac atcccctttt aacagctgac
    13861 ctctctaccg gagccgtgcg ctccggtttt cccttctttt tacatcctgt acatcacatc
    13921 aaatcattaa acttgcatta acttatttat aacttatatt gtcacatgca aatcagaatg
    13981 gtttcgaatg aaagttaatg agaggtggtt atggcttcta cttttaccag cgacacattg
    14041 cctgccgatc acaaagcagc tatccgtcag atgaagcacg cgctgcgggc gcagcttggc
    14101 gacgtccagc agatctttaa tcagctaagc gatgacattg ccacgcgagt ggctgaaatc
    14161 aacgcactca aagcacaggg cgatgccgtc tggccggtgc tgtcttatgc cgatatcaaa
    14221 gcaggtcatg ttactgcaga gcagcgcgaa cagattaaac gtcgcggttg tgcggtgata
    14281 aaaggccatt tcccccgcga acaagcgcta ggctgggatc agtcgatgct ggactatctg
    14341 gaccgcaacc gctttgacga ggtctacaaa ggccccggcg ataatttctt cgggacgctc
    14401 agcgcttcac gtcccgagat ttaccccatc tactggtcgc aggcgcaaat gcaggcccgc
    14461 cagagtgaag aaatggcgaa tgcgcagtcg tttctcaatc gtctgtggac atttgaaagt
    14521 gatggaaagc aatggtttaa cccggatgtg agcgtcatct accctgaccg tatccgccgc
    14581 cgtccgcctg gaacgacctc caaaggtctt ggagcgcata ccgactccgg ggcgctggaa
    14641 cgctggctgc ttccagcgta tcagcgcgtt ttcgccaacg tctttaatgg caatctggcg
    14701 caatatgatc cctggcatgc ggcacatcgt acggaagttg aagagtacac ggtggacaac
    14761 accaccaaat gttccgtgtt tcggacattc cagggctgga cagcgctctc tgatatgctg
    14821 cctggtcagg ggttgctgca cgttgtgccc attcctgaag ccatggcgta cgtactgtta
    14881 cgtccgctgc ttgatgatgt gccggaggat gaactgtgcg gcgtagcgcc cggaagagtg
    14941 ttgccggtat cagagcaatg gcatccactg ttaattgagg cgttaaccag cattccaaaa
    15001 ctcgaggccg gagactccgt ctggtggcac tgcgacgtca tccattccgt tgcccccgtt
    15061 gaaaatcaac agggttgggg caacgtgatg tacattcctg cggcaccgat gtgcgagaaa
    15121 aatcttgcct acgcgcacaa ggtgaaggcc gcactggaaa aaggcgcatc gccgggcgac
    15181 ttcccgcgcg aggactatga aacaaactgg gaaggacgct ttacgcttgc cgacctcaac
    15241 attcacggta agcgagcgtt gggcatggat gtttgagagt taatggaagt gaattgatgt
    15301 gggattaaga aaatcgattc gacgttactc agattgctga caagtgcgct ttcttaatgc
    15361 ctgatgcgct acgcttatct ggcctacaaa gtcctgcaga ttcaatatat tacaggtac
//
LOCUS       DAEVZA010000114        15373 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEVZA010000114 DAEVZA010000000
VERSION     DAEVZA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.114"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            299..1213
                     /gene="sitA"
                     /locus_tag="LAF92_003729"
     CDS             299..1213
                     /gene="sitA"
                     /locus_tag="LAF92_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBI9792665.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            1213..2040
                     /gene="sitB"
                     /locus_tag="LAF92_003730"
     CDS             1213..2040
                     /gene="sitB"
                     /locus_tag="LAF92_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBI9792666.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            2037..2894
                     /gene="sitC"
                     /locus_tag="LAF92_003731"
     CDS             2037..2894
                     /gene="sitC"
                     /locus_tag="LAF92_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBI9792667.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            2891..3748
                     /gene="sitD"
                     /locus_tag="LAF92_003732"
     CDS             2891..3748
                     /gene="sitD"
                     /locus_tag="LAF92_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBI9792668.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(3982..4239)
                     /locus_tag="LAF92_003733"
                     /pseudo
     CDS             complement(3982..4239)
                     /locus_tag="LAF92_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            4237..4650
                     /locus_tag="LAF92_003734"
                     /pseudo
     CDS             4237..4650
                     /locus_tag="LAF92_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            4991..5371
                     /gene="crcB"
                     /locus_tag="LAF92_003735"
     CDS             4991..5371
                     /gene="crcB"
                     /locus_tag="LAF92_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBI9792669.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(5751..6944)
                     /locus_tag="LAF92_003736"
     CDS             complement(5751..6944)
                     /locus_tag="LAF92_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9792670.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            7080..8804
                     /gene="iucA"
                     /locus_tag="LAF92_003737"
     CDS             7080..8804
                     /gene="iucA"
                     /locus_tag="LAF92_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBI9792671.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            8805..9752
                     /gene="iucB"
                     /locus_tag="LAF92_003738"
     CDS             8805..9752
                     /gene="iucB"
                     /locus_tag="LAF92_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBI9792672.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            9752..11494
                     /gene="iucC"
                     /locus_tag="LAF92_003739"
     CDS             9752..11494
                     /gene="iucC"
                     /locus_tag="LAF92_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBI9792673.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            11491..12768
                     /gene="iucD"
                     /locus_tag="LAF92_003740"
     CDS             11491..12768
                     /gene="iucD"
                     /locus_tag="LAF92_003740"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBI9792674.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            12850..15051
                     /gene="iutA"
                     /locus_tag="LAF92_003741"
     CDS             12850..15051
                     /gene="iutA"
                     /locus_tag="LAF92_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBI9792675.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         3681 a         3879 c         3939 g         3874 t
ORIGIN      
        1 tcccttcatt tagtgactgc ccctgggttt ttcatgctct cctgcgtcct agagaacttg
       61 ccaataacct acattatttt tgctttgcca ttaccaatcg tcctgaacaa gttcatttta
      121 aagccaaagc gctgcccaac ttttatcctc aataccacat cgtcagcagg aaataaccta
      181 atccctgtgt tgggatctta taatcgctaa tgataattat ttacatttta tagcttctgc
      241 tataaaacat agtagttgct atgtttattc taattttgct cactataggt actaaatcat
      301 gctctcaata aaaaaagtaa ccatgctctt ggggtgccta gtcctcacct gctcgatagc
      361 atttcaggcg agtgcagctg aaaaattcaa ggtcattaca acttttacca tcatcgcaga
      421 tatggccaaa aacgtggctg gagatgctgc agaagtctca tccataacca agcctggtgc
      481 agaaattcat gagtatcagc ctacccctgg cgatattaaa cgtgcgcagg gggcacaact
      541 gattctcgcc aatggtatga atctggaatt gtggttccaa cgcttttacc agcatctcaa
      601 tggggttcca gaagtaattg tctcttcggg tgtgacgcca gtagggatca ccgaagggcc
      661 ctatgagggc aaacctaacc cccatgcctg gatgtcgcca gataatgctc tgatttacgt
      721 cgataatatt cgtgatgcgt ttataaaata cgatccggca aatgcacaaa cctaccaacg
      781 taatgccgat acttataaag ccaagattac ccaaaccctt gcccccctgc gtaagcagat
      841 tgcggaactc cctgagaatc agcgatggat ggtcaccagt gaaggggctt tttcttatct
      901 cgcacgcgat ttggggctaa aagagcttta tctgtggccg attaatgccg atcaacaagg
      961 aacaccgcag caggtacgta aggttgttga tatagttaag aaaaatcata tcccggcagt
     1021 ctttagcgag agtacgattt ccgataaacc agcgcgtcag gttgcgcgtg aaaccggcgc
     1081 gcactacggt ggtgtgctct atgtcgattc cctgagcaca gaaaacggcc cggtacctac
     1141 gtatatcgac cttctgaagg ttactaccag tacgctggta cagggaatta aagccggaaa
     1201 aagggagaaa taatgatgca atctgcaggc attgtcgtta atgatgttac tgtcacctgg
     1261 cgtaacgggc acacagcact gcgcgatgca tccttcacag taccaggcgg atctattgcc
     1321 gctctggttg gggtaaacgg ttccggaaaa tcgacgttgt ttaaagcgat tatgggattt
     1381 gtgcgtctga ccagcgggaa gatatctgtt ctgggtattc ccacacgaca ggcgctacag
     1441 aaaaacctgg ttgcctacgt ccctcagtca gaagaggttg actggtcatt tcctgtactg
     1501 gtggaagatg tggtgatgat gggccgctat gggcatatgg gtatgttgcg tatcgccaaa
     1561 aagagggatc gtcagattgt caccgatgcg ctggaacgtg tcgatatggt ggattttcgc
     1621 catcgacaaa tcggcgagct ttccggtgga caaaagaaac gcgtctttct ggcgagagcg
     1681 attgcacagc agggtgatgt gatcctgctg gatgaacctt ttaccggcgt tgatgtgaaa
     1741 accgaagcga aaatcatcag cctgttgagg gagttacgcg cggaaggcaa aacgatgctt
     1801 gtctcaacgc acaatctcgg atccgtcacg acattttgcg attatacggt catggtcaaa
     1861 ggcaccgtcc tggcaagcgg gccgacagac accactttta cagccgagaa cctggagctg
     1921 gcttttagcg gcgtactgcg ccatgtcaca cttaacggct cggaagaaag cattattact
     1981 gacgatgaac gcccttttgt ggcacatcga ccgtcagcgg tgcagaggga agaaagatga
     2041 acgtgctact ggaacccttc agctatgaat atatgctcaa tgcgatgtgg gtctcggcga
     2101 tggtcggcgg tctctgcgct ttcctgtcat gctatctgat gctaaaaggc tggtcgctga
     2161 ttggcgatgc gctgtcgcac tctattgtcc cgggtgttgc gggggcatat atgctggggc
     2221 ttccgttttc tctgggagcc ttcttttccg gcggactggc agcgggcagc atgctgtttc
     2281 ttaaccaact cactcgctta aaagaagatg ccatcatcgg cctgatcttc tcgtcctttt
     2341 ttggcctggg gctgtttatg gtgtcattga acccgacatc cgtgaacatt cagaccatcg
     2401 tcctgggcaa tattctggca atcgatccgg cggatatcct gcaactcact atcattggca
     2461 ttctctcgat catcgtgctt tttttcaaat ggaaagatct tatggtgacc tttttcgacg
     2521 agaatcacgc ccgtgctatt ggattgcatc cgggcagatt aaaaatcctc ttcttcaccc
     2581 tgctgtcggt ttcgaccgtt gcggcgctgc aaacggttgg tgcctttctg gtgatctgtc
     2641 tggtggtgac tcccggtgca acagcatggt tgttaaccga tcgttttccc cgcctgctca
     2701 tgattgctgt aactattggc agtgtgacca gtttcctcgg cgcgtgggtc agttactttc
     2761 tggacggcgc gacaggtgga atcatcgtgg tggcgcagac actgctgttt cttttagcat
     2821 ttgtctttgc gcccacccat gggcttttgg caaatcgtcg gcgtgcgcat aaagcgctgg
     2881 aggatcgctc atgatggcat tattactgga gccactgcag ttcaccttta tgagtcacgc
     2941 actgctgatt tcactggttg tttccatacc ctgtgcgctg ctgtcggtct ttctggtcct
     3001 gaaaagttgg gcactgatgg gtgacgcgat gagtcatgcg gtttttcctg gcatcgtgct
     3061 tgcctggatt ctggggttac ctctggctac aggggcattc gtcgccggag tattctgtgc
     3121 ggtggcgacg ggatacctga aagacaacag ccgaatcaag caagatacag tgatggggat
     3181 tgttttttct ggcatgtttg ccgcaggtct gatcttgtat attgcagtca aaccagacgt
     3241 acatcttgat catattcttt tcggcgacat gctcgggata accatcggcg atataatcca
     3301 gacggtgatt attgccggac tggttacact tgttattagc gtaaaatggc gggatttttt
     3361 gctgttcagc tttgattatc aacaggcgca ggcaagcggt ttgcatacga gatggctgca
     3421 ttatgggctg ctgtgcatgg tctccctgac cattgtggcg acgctgaaag cggtaggtat
     3481 cattctctct atttctcttc tcattgcgcc tggtgccatt gcggtactcc tgacacaacg
     3541 ctttcatatt gcgctcctgc tggcgactgg catatcgata ttggtctcaa tgactggtgt
     3601 ctggctgtcc ttttttatcg acagcgcgcc agctccgaca attgttgtct tattcgcggt
     3661 catgttcatc atgacgttta ccgttaccag catcaacgca cgcacaaagg aaaatgccga
     3721 accacgggat ttgttatcat ccgattagca gaacactcgg ccagcaacat ccgcttattg
     3781 gcgatgcgct tgaacgaata atgcacagac ttatgattct gataagtttt gttgccagag
     3841 agttgctgcg gactggatag caagtaatgt cgattctcag gggtagcaaa cagcgcagta
     3901 aaatgaggga agtagtggca aaacatagag aaaattgggg ggcgagggcc agtgatgtaa
     3961 aaaaccacgc taagagttta attaattgtt tgtattgaag tttaatgatc ttaactcacc
     4021 cttaagaata tcttccggta acgaagacgt ataatgcccc agcatattta tatggctatg
     4081 tatcagcagc gataatcttg ccggatgcta ctcctcagga gtttctcccg atttgcgaag
     4141 atgtgacaac gcctcctgca tgtaaagtgt gtttcacagc acaaccgcat tggtcaccag
     4201 ccccagagca cctagttgat cttcctgccc ttaacggtag ctgaagatga ctgggctgga
     4261 tggaaaatcc tgcatggtgc attgggagag cagatagaat tggttggcga cgatctgttt
     4321 gtgaccaacg taaagtatat tcagcgcggc attgacgaaa accttgccaa ttcagcattg
     4381 attaaactca accagattgg ttccctgagt gaaacctttg atgcggtaca actttgccat
     4441 gacaacaact ggggcacctt tatctcacac cgcagtggtg agacagtgga cagctttatc
     4501 gccgatatga cagttgcaat gcgcgcaggt catctcaaaa cgggagctcc atgccggggt
     4561 gagcgtattg aaaaatataa ccagcttatg agaattgagg atgaactggg ttcatcggcg
     4621 caatttgccg gaaagtctgc ctttaaatga cattattgtg ccaatccccc aggcttacac
     4681 gtcggggtta ttctggagga atgatgaaag ctcacgtttt tgttatttct atgcttactg
     4741 gcattgtcgt cacctatgcc gtgttacttc ttggttgctt gttcatcgat aaaacgctcc
     4801 ccactgtaga tgttgtcatt ctgtcacttg tcgtgggggc ttctgctcag caattgtcga
     4861 gggtattaat gtcaataaat agaaccttcc cttatcttgc atgtagtcaa aggatataaa
     4921 ttaacaatat cagtcaaatg agaacatatt ctgatattgt tacgataaaa aattatttta
     4981 tgaggggtac atgattaaat cactttttgc cgtcattatc ggcggttctg ttggatgtac
     5041 tctgcgctgg ttactgtcca cacgctttaa cagtcttttt ccaaatctac ctccaggtac
     5101 actggtggtg aacctccttg ccgggttaat tattggtact gcgttggcgt actttttacg
     5161 acaaccacac cttgatcctt tttggaaatt aatgataaca acgggacttt gtggaggtct
     5221 gagtactttc tctacatttt ctgttgaagt attcgcatta ctccaggcgg gaaattatat
     5281 atgggctttg acgtctgtac ttgttcacgt cattggctcc ctcatcatga cagctttagg
     5341 cttttttatc ataaccatac ttttcgcatg agaattcatt aacgttacag gtttatcaac
     5401 taatgtgacg cttaggatag aatttcgctc catggtatgc gtttgcagtc attacagtct
     5461 ccatgacgac tgtaatgact gcaaacgcat tacctgacat atcagtataa acaatgaatt
     5521 aacctcttgg cgtatttttt acatcactgg ccctcacccc ccaaaattct ctctgaaaaa
     5581 ttgttgcggt taacgttgcc aatgtatcca cccatttcag aagacgatct ggcatcctgg
     5641 tggcatgctg cgatgtgctt caggtgagtg cactgggcgg tcagcacagg cggtttcgac
     5701 agcgaaattg cacaggacgt tatgccagct gtgagtacaa catcatagcc ttacttgatt
     5761 gtacggtaat gccaaataca tgcagacact atggtaaata atacagcggc aaataatgcc
     5821 gtcacaatca gcgcgattga gacacctgtt ttcgacgcca gtggcaccaa caatgcgcac
     5881 agcccatagc caagtgtgtg actggccgca agccagcccg cgcctgcgcc gtcggccagc
     5941 ttatcattca gtaaaagttg ataagcgggt gttgccagag cggccccaaa tgacagcact
     6001 gcacagccca ggtaaaacaa ccatagctgt tcagtgagca tgatagccag accactactc
     6061 atcaacaccc cggcactcag gagcagagcc actggagtca ggcgctgtgg acggagtacc
     6121 acgaactgcg cgataagcgc agctattgcg gacagcccca acaaccacgc cacctgttgg
     6181 ctaatagtgg tggtatcagt atcgaactgg cgagtaaggg ctggcgaaag tccaagctgc
     6241 atcatgctca ttgccgcagc cagtaacatt gcgcaaagca gataaggcag gcaatcccgc
     6301 ttcaggctga cgctcttgcg ctccggtgtg ggctgtggtg gtgtgccggg caaccgcaga
     6361 agcatcacca cagcccacac cggagctgcc atcagcatcc acactggcgc cagagggtga
     6421 atgaccaaca ttgccgccgc gcacagcggc ccgaatagcc tgccgcagct caggccggag
     6481 ctgatggttg ccagagcggc catgcgattc ccttctcccg ctctctgcaa tgcccagacc
     6541 tgacaggctg gcaccattgc tgacacggtc agaccgtaga cgatccgggc gatgattaat
     6601 attcccaacc ccaccgctgt tgttacggca ccggtagcca tcagcacgct tcccagccca
     6661 agcagggtaa agcttacgcc atatcctccc agagcccaca gcaccacagt cttactgcca
     6721 gtaagggaaa tttgctttcc ccaccatggg gaagatggca gaaacagcat tgagcccagc
     6781 atcagcaggg cagcccatac agacagactc agatttgtct gtatgaccag aacagggagt
     6841 acaaccagca gaccgttctg tccgataccg agaagaccgg cactgaacgc cagtggccag
     6901 caggacaggg ttttttgggg cgtatcttcg atcttagatg acattgtaat gtttcaacac
     6961 catgtattaa ttgtgtttat ttgtaaaatt aatttttctg acaataacat ttctcattga
     7021 taatgagaat cattattgac ataattgtta ttattttact gtgtaggagc tgtttgatta
     7081 tgatcctgcc ctctgaaaaa tccgccacag atgtggctgc gcagtgcttc ctgaatgcct
     7141 tgattcgtga aaccaaagac tggcaactgg cggaataccc gccagacgaa ttgattatcc
     7201 cgctggacga acagaagtcg ctccatttca gagtggctta tttctcccca acccagcatc
     7261 accgctttgc atttcctgct catctggtca cggcatcagg cagttatcct gtcgacttta
     7321 ccactctctc ccggcttatt attgataagc tccggcatca actttttctg ccagttcccc
     7381 tctgcgaaac tttccaccaa cgcgtgctgg aaagctacgc ccatacgcaa cagacaattg
     7441 atgcccgtca tgactgggcc atcctgcgtg aaaaagcgtt gaattttggc gaggctgagc
     7501 aggcactgct gacaggacac gctttccacc ctgcacctaa gtctcatgaa ccgtttaacc
     7561 gccaggaagc tgaacgatac ctgcctgaca tggcacctca cttcccgctg cggtggtttt
     7621 cggtggataa aacgcaaatc gctggtgaaa gtctgcatct taaccttcaa cagcggttga
     7681 cgcgatttgc cgcagaaaat gcgccccagt tactcaacga attaagtgac aatcaatggc
     7741 tgttcccgct gcacccgtgg cagggagaat atcttttgca gcaagtgtgg tgccaggcac
     7801 tttttgctaa aggacttatc agagacttag gcgaggccgg cacgtcgtgg ctgccgacca
     7861 cctcttcccg ctccctctac tgtgctacca gccgcgatat gatcaagttc tccctgagcg
     7921 ttcggctgac caactccgtc cgtactctgt ctgtgaaaga ggtggagcga ggaatgcgcc
     7981 tggcacgtct ggcgcaaacc gacggctggc agatgctaca ggcccgcttc cctactttcc
     8041 gggtaatgca ggaggacggc tgggccgggc tgcgcgatct taacggcaac atcatgcagg
     8101 aaagtttgtt ttccctgcgt gaaaacctgc tgctggagca gccgcaaagc cagaccaacg
     8161 ttctggtttc cctgactcag gccgcaccgg atggcggtga ttcgctgctg gtttcggcgg
     8221 taaaacgcct gagcgatcgc ctcggtatca ctgtgcaaca ggccgcccac gcatgggtcg
     8281 atgcttactg tcagcaggtg ctgaagcctc tgtttacggc tgaagcggat tacggcctgg
     8341 tgctgctggc gcatcagcaa aatattcttg tgcagatgct tggggatctg ccggtcggat
     8401 ttatttaccg tgactgccag ggcagcgcgt ttatgcctca cgcgacagag tggctcgata
     8461 ccattgacga ggcgcaggcg gaaaatattt tcacccgtga gcagttgctg cgctatttcc
     8521 cttattacct gctggttaac tccacttttg ccgtaactgc tgcgctgggt gctgccgggc
     8581 tggacagcga agcgaatctg atggctcgtg tacgaacatt gctggctgaa gtgcgtgacc
     8641 aggtgactca taaaacctgt ctcaactatg ttctggaaag tccgtactgg aacgtaaaag
     8701 gtaacttttt ctgttatctg aacgatcata acgagaacac catcgttgac ccttcggtga
     8761 tctattttga tttcgctaac ccgctgcagg ctcaggaggt ctgaatgtct ggggcaaaca
     8821 ttgttcacag cggatatgga ctgcgctgtg aaaaactcga caagcctctg aatcttggct
     8881 gggggctgga caatagcgcg gtgttgcact ggcccgggga gctgccaaca gggtggctgt
     8941 gcgacgcgct ggatcagata tttatcgccg caccacaact ttcagcagtg gttcttccct
     9001 ggtccgaatg gtgtgaggag ccacaggcgc tgacgctttt cggacaggta caaagcgaca
     9061 ttatccatcg ttccgctttc tggcagttac cgttatggct gagttctccg gcaaaccggg
     9121 cctccggtga aatggttttt gatgcagagc gtgagattta tttcccgcag cgcccccccc
     9181 gtccgcaggg tgaagtttat cgtcgttacg atccacgcat tcgcaggatg ctgagtttcc
     9241 gcattgccga tcccgtttct gatgcagaac gtttcactcg ctggatgaac gatccgcgcg
     9301 ttgagtattt ctgggagcaa agtggctcac tggaggtaca gatcgcctat ctggaacgcc
     9361 agctcaccag taaacatgca ttcccgctga ttggttgttt cgacgatcgg ccgttcagtt
     9421 atttcgaaat ctactgggcg gcggaagacc gcattggtcg ccactattcg tggcagccct
     9481 ttgaccgtgg cctgcatctg ctggttggtg aacagcaatg gcgcggagcc cactatgtgc
     9541 aaagctggct gcgcggggtg acacattacc tgctgctgaa tgagccacgt acgcagcgca
     9601 cagtactgga gccacgcacc gataaccagc gcctgttccg ccatcttgag cctgcgggat
     9661 accggacaat taaagagttc gacttcccac ataaacgctc gcgcatggtg atggcggatc
     9721 gtcatcactt cttcacggag gtcggtctgt aatgaatcac aaagactggg atttggtcaa
     9781 ccgccgcctg gtggcaaaaa tgttgtctga gctggagtat gagcaggttt tccacgcgga
     9841 atctcagggc gatgaccgct actgcattaa cctgccggga gcacaatggc gcttcatcgc
     9901 tgaacgtggt atctggggct ggctctggat tgatgctcaa actctgcgct gcgcggacga
     9961 gccagtactg gctcagacgc tgctgatgca gctaaagcag gtactgtcaa tgagcgatgc
    10021 aaccgttgct gagcatatgc aggatttgta tgccacgctg ctgggcgacc tgcaactact
    10081 gaaagcccgt cgcgggctga gcgccagtga cctgattaat cttaatgccg accgcctgca
    10141 atgcctgctg agcggtcatc ctaaattcgt ttttaataaa ggtcgccgtg gctggggtaa
    10201 agaggcgctg gaacgatatg cgccagagta tgccaacacc ttcagactgc actggctggc
    10261 ggtaaaacgt gaacatatga tctggcgctg tgataacgag atggatattc atcagttgtt
    10321 gacggccgca atggatccgc aggagtttgc ccgcttcagt caggtctggc aggaaaacgg
    10381 actggatcat aactggctgc cgctgccggt acatccgtgg cagtggcagc aaaaaatcgc
    10441 taccgacttc atcgctgatt ttgccgaagg caggatggtg tctctcggcg agtttggcga
    10501 ccagtggctc gcccagcagt cgctgcgtac cctgaccaac gccagtcgcc ggggcgggct
    10561 ggatatcaag ctgccgctga ccatctacaa cacctcatgc taccggggga tccctggcag
    10621 atacatcgct gccggaccac tggcttcacg ctggctacaa caggtttttg cgaccgacgc
    10681 caccctagtg caaagcggcg cagtgatact tggtgaaccg gctgcaggct atgtctccca
    10741 tgaaggctat gccgcgcttg cccgggctcc ctatcgctac caggaaatgc ttggtgttat
    10801 ctggcgggag aatccgtgcc gctggctgaa accggatgaa agtccggttc tgatggcaac
    10861 actgatggag tgcgacgaaa acaatcagcc gctggcaggc gcatatatag accgctccgg
    10921 gctggacgct gaaacctggc ttacgcaact gttccgggtg gtcgtggttc ctctgtatca
    10981 cctgctttgc cgctacggtg tcgcgcttat tgcacatgga caaaatataa ctctcgccat
    11041 gaaagagggg gttccacagc gtgttctgct gaaagacttc cagggcgata tgcgactggt
    11101 gaaagaagag ttccccgaaa tggactcttt gcctcaggag gttcgtgatg ttacatcccg
    11161 cctgagtgcg gactacttaa tccatgattt gcagacgggt cacttcgtga cagtactgcg
    11221 ttttatttcg ccactgatgg ttcgtcttgg cgtacctgaa aggcgatttt atcaactgct
    11281 ggcagcagtg ttgagtgatt acatgaaaaa acatccacaa atgtcagagc gttttgcgct
    11341 tttctcactt ttcaggccac aaatcattcg cgtggtgctg aacccggtaa aactgacctg
    11401 gcctgatctg gatggcggca gccgcatgct gccgaattac cttgaggatc tgcaaaatcc
    11461 gctgtggctg gtaactcagg aatatgaatc atgaaaaaaa gtgtcgattt tattggtgta
    11521 gggacagggc catttaatct cagcattgct gcgttgtcac atcagatcga agaactggac
    11581 tgtctcttct ttgatgaaca tcctcatttt tcctggcatc cgggtatgct ggtaccggat
    11641 tgtcatatgc agaccgtctt tctgaaagat ctggtcagtg ctgttgcacc tacaaatccc
    11701 tacagttttg ttaactatct ggtgaagcac aaaaagttct atcgcttcct tacaagcaga
    11761 ctacgtacag tatcccgtga agagttttct gactacctcc gctgggctgc tgaagatatg
    11821 aataacctgt atttcagtca taccgttgaa aacattgatt tcgataaaaa acgtcgattg
    11881 tttctggtgc aaaccagcca gggagaatat tttgcccgca atatctgcct tggtacagga
    11941 aaacaacctt atttaccacc ctgtgtgaag catatgacac aatcctgttt ccatgccagt
    12001 gaaatgaatc ttcgtcggcc ggaccttagt ggaaaacgga taaccgtggt tggtggagga
    12061 cagagtggtg cagacctgtt ccttaatgca ttacgcgggg aatggggaga agcggcggaa
    12121 ataaactggg tctcccggcg taataatttt aacgcactgg atgaggctgc ttttgctgat
    12181 gagtatttta cacctgaata tatttcaggc ttctccggac tggaggaaga tattcgccat
    12241 cagttactgg atgagcagaa aatgacatcg gatggcatca ctgccgattc tttactgacc
    12301 atttatcgtg agttgtacca ccgttttgaa gttctgagaa aaccaagaaa tatccgtctg
    12361 ctacccagcc gctcggtaac aactctggaa agtagtggtc ctggctggaa gttgctgatg
    12421 gagcatcatc tggatcaggg cagggagagc ctggaaagtg atgtggtgat tttcgccaca
    12481 ggttaccgtt ctgcgttgcc acaaatactt ccctcactga tgcccctgat caccatgcac
    12541 gataagaaca cctttaaagt gcgtgatgac ttcactctgg aatggagtgg cccgaaagag
    12601 aacaacatct tcgtggtcaa cgccagtatg caaacccatg gcatcgccga accccagctc
    12661 agcctgatgg catggagatc tgcacgtatt cttaatcgcg taatgggacg tgatttattc
    12721 gatctcagta tgccgcccgc cctgattcag tggcgcagcg gcacctaggg aaaacgcagc
    12781 cggaggctgc ttctttaact cgctacacag catctttggg ctgatttttt ccgcccgtat
    12841 ggaggaataa tgatgataag caaaaagtat acgctttggg ctctcaaccc actgcttctt
    12901 accatgatgg cgccagcagt cgctcaacaa accgatgatg aaacgttcgt ggtgtctgcc
    12961 aaccgcagca atcgcaccgt agcggagatg gcgcaaacca cctgggttat cgaaaacgcc
    13021 gaactggaac agcagattca gggcggcaaa gagcttaaag acgcactggc tcagctgatc
    13081 cctggccttg acgtcagcag ccggagccgc accaactacg gtatgaatgt gcgtggccgc
    13141 ccgctggtcg tgctggttga cggcgtgcgt ctcaactctt cacgtaccga cagccgacaa
    13201 ctggactcta tagatccttt taatatcgac catattgaag tgatctccgg tgcgacgtcc
    13261 ctgtacggcg gcggcagtac cggtggcctg atcaacatcg tgaccaaaaa aggccagccg
    13321 gaaaccatga tggagtttga ggctggcacc aaaagtggct ttagcagcag taaagatcac
    13381 gatgaacgca ttgccggagc tgtctccggc ggaaatgagc atatctccgg acgtctttcc
    13441 gtggcatatc agaaatttgg cggctggttt gacggtaacg gcgatgccac cttgcttgat
    13501 aacacccaga ccggcctgca gtactccgat cggctggaca tcatgggaac tggtacgctg
    13561 aacatcgatg aatcccggca gcttcagttg atcacacagt actataaaag ccagggcgac
    13621 gacgattacg ggcttaatct cgggaaaggc ttctctgcca tcagagggac cagcacgcca
    13681 ttcgtcagta acgggctgaa ttccgaccgt attcccggca ctgagcggca tttgatcagc
    13741 ctgcagtact ctgacagcgc ttttctggga caggagctgg tcggtcaggt ttactaccgc
    13801 gatgagtcgt tgcgattcta cccgttcccg acggtaaatg cgaacaaaca ggtgacggct
    13861 ttctcttcgt cacagcagga caccgaccag tacggcatga aactgactct gaacagcaaa
    13921 ccgatggacg gctggcaaat cacctggggg ctggatgctg atcatgagcg ctttacctcc
    13981 aaccagatgt tcttcgacct ggctcaggca agcgcttccg gagggctgaa caacaagaag
    14041 atttacacca ccgggcgcta tccgtcgtat gacatcacca acctggcggc cttcctgcaa
    14101 tcaggctatg acatcaataa tctctttacc ctcaacggtg gcgtacgcta tcagtacact
    14161 gaaaacaaga ttgatgattt catcggctac gcgcagcaac ggcagattgc cgccgggaag
    14221 gctacatccg ccgacgccat tcctggcggc tcagtcgatt acgacaactt cctgttcaac
    14281 gccggtctgc tgatgcacat caccgaacgc cagcaggcat ggctcaactt ctcccagggc
    14341 gtggagctgc cggacccggg taaatactat ggtcgcggca tctatggtgc tgcagtgaac
    14401 ggccatcttc ctctaacaaa gagtgtgaac gtcagcgaca gcaagctgga aggcgtgaaa
    14461 gtcgattctt atgagctggg ctggcgcttt actggcaata atctgcgtac ccaaatcgcg
    14521 gcctactatt cgatttctga taagagcgtg gtggcgaata aagatctgac catcagcgtg
    14581 gtggacgaca aacgccgtat ttacggcgtg gaaggtgcgg tggactacct gattcctgat
    14641 actgactgga gtaccggagt gaacttcaac gtgctgaaaa ctgagtcgaa agtgaacggt
    14701 acctggcaga aatacgatgt gaagacagca agcccatcaa aagcgacagc ctacattggc
    14761 tgggcaccgg acccgtggag tctgcgcgtg cagagcacca cctcctttga cgtgagcgac
    14821 gcgcagggct acaaggtcga tggctatacc accgtggatc tgctcggcag ttatcagctt
    14881 ccggtgggta cactcagctt cagcattgaa aacctcttcg accgtgacta caccactgtc
    14941 tgggggcagc gtgcaccact gtactacagc ccgggttacg gcccagcgtc actgtacgac
    15001 tacaaaggca ggggccgaac ctttggtctg aactactctg tgctgttctg accggtattc
    15061 ctttacaaca aaggtacgct gatatcaaca tggccgctga cagccaagtt gatatcatat
    15121 aatacacgac ataatctgta gtcagggagg atagactctt tactgactac agattatgtc
    15181 gtgttccgtg ctcatttcct caaaaaaata caagaaaaga attagtattc taacaaaaag
    15241 tgaaataaat tgtatcaaac tccctctttt aatcctgttg agtaaatcag cttttgcaat
    15301 aggattgaaa gagtgtaagt ggaatctctt ccggatactc gttaccaccg tggctagaat
    15361 atctacggct gcg
//
LOCUS       DAEVZA010000115        15324 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEVZA010000115 DAEVZA010000000
VERSION     DAEVZA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.115"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            255..1472
                     /gene="alaA"
                     /locus_tag="LAF92_003742"
     CDS             255..1472
                     /gene="alaA"
                     /locus_tag="LAF92_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBI9792676.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1556..2155
                     /gene="yfbR"
                     /locus_tag="LAF92_003743"
     CDS             1556..2155
                     /gene="yfbR"
                     /locus_tag="LAF92_003743"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBI9792677.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2214..4046)
                     /locus_tag="LAF92_003744"
     CDS             complement(2214..4046)
                     /locus_tag="LAF92_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBI9792678.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4133..4783)
                     /gene="hxpA"
                     /locus_tag="LAF92_003745"
     CDS             complement(4133..4783)
                     /gene="hxpA"
                     /locus_tag="LAF92_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBI9792679.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4794..5288)
                     /locus_tag="LAF92_003746"
     CDS             complement(4794..5288)
                     /locus_tag="LAF92_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBI9792680.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5371..5826)
                     /locus_tag="LAF92_003747"
     CDS             complement(5371..5826)
                     /locus_tag="LAF92_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBI9792681.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6164..7366
                     /gene="ackA"
                     /locus_tag="LAF92_003748"
     CDS             6164..7366
                     /gene="ackA"
                     /locus_tag="LAF92_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBI9792682.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7441..9585
                     /gene="pta"
                     /locus_tag="LAF92_003749"
     CDS             7441..9585
                     /gene="pta"
                     /locus_tag="LAF92_003749"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBI9792683.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9775..11295
                     /gene="yfcC"
                     /locus_tag="LAF92_003750"
     CDS             9775..11295
                     /gene="yfcC"
                     /locus_tag="LAF92_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBI9792684.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11328..11870)
                     /gene="yfcD"
                     /locus_tag="LAF92_003751"
     CDS             complement(11328..11870)
                     /gene="yfcD"
                     /locus_tag="LAF92_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBI9792685.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11928..12479)
                     /gene="yfcE"
                     /locus_tag="LAF92_003752"
     CDS             complement(11928..12479)
                     /gene="yfcE"
                     /locus_tag="LAF92_003752"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBI9792686.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12535..13179)
                     /gene="yfcF"
                     /locus_tag="LAF92_003753"
     CDS             complement(12535..13179)
                     /gene="yfcF"
                     /locus_tag="LAF92_003753"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBI9792687.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13315..13962
                     /gene="yfcG"
                     /locus_tag="LAF92_003754"
     CDS             13315..13962
                     /gene="yfcG"
                     /locus_tag="LAF92_003754"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBI9792688.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14019..14381
                     /gene="folX"
                     /locus_tag="LAF92_003755"
     CDS             14019..14381
                     /gene="folX"
                     /locus_tag="LAF92_003755"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBI9792689.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKDEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14402..15295
                     /locus_tag="LAF92_003756"
     CDS             14402..15295
                     /locus_tag="LAF92_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBI9792690.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
BASE COUNT         3800 a         4101 c         3790 g         3633 t
ORIGIN      
        1 cacacaatta tcttacagat aaaaaaacca gacttacaat taagaatcag aacaattcac
       61 tatataacat tgcatgtaaa gcatatacac ctcattattt tgtcattatt aagttattaa
      121 cagcacaatc gagccttccc ctctggcaaa atcttattct gcagaccttc aaaacaccgt
      181 cctgggggag tacattgttc taagctgact tccacggcag ggagtggcga taacagcaaa
      241 aaaggtcaag attcatgtcc cccattgaaa aatccagcaa attagagaat gtctgttatg
      301 acatccgtgg tccggtgctg aaagaagcaa aacgcctgga agaagaaggt aacaaggtac
      361 tgaaactgaa catcggcaac ccagccccgt tcggttttga cgcgccagat gaaatcctcg
      421 ttgacgtgat acgcaacctg cctaccgctc aagggtattg cgattccaaa ggtctttact
      481 ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg catgcgtgat gttaccgtgg
      541 aagatattta catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc
      601 tgaacagcgg ggacgaaatg ttggttcctg caccagatta cccactatgg accgcggcgg
      661 tttcgctttc cagcggtaaa gcggtgcatt atctttgcga tgaatcctct gactggttcc
      721 cggacctcga tgatattcgc gctaaaatta cgcctcgtac gcgtgggatc gttattatca
      781 acccaaataa cccaaccggc gcggtatatt ccaaagagct tttaatggag attgtggaga
      841 ttgcacgtca gcataatctc attatcttcg ccgatgaaat ttatgacaaa attctttacg
      901 acgacgctga gcatcactca attgcgccgc tggcacctga cctgctgacc attaccttta
      961 acggactgtc gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg
     1021 ggccgaaaaa acacgccaaa ggctacatcg aaggtctgga aatgctggct tcaatgcgcc
     1081 tgtgtgctaa cgttcctgcg caacacgcca ttcagaccgc gctgggtggt tatcagagca
     1141 tcagtgaatt tattacccct ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaac
     1201 tgatcaacga tattccgggc gtttcctgcg tgaaacctcg tggtgcgctg tatatgttcc
     1261 cgaaaatcga cgccaaacgc tttaacattc acgacgatca gaaaatggtg ctggatttcc
     1321 tgttgcagga aaaagttctg ttggtgcaag ggacggcatt caactggccg tggccggatc
     1381 acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg
     1441 cgcgtttcct ttctggttat catcagctgt aatcttaatt tcactgccgg agattgcatc
     1501 cggcagcgtt atcccgccac aatgacctga tgatgtcatc atacgtaagg tcactatgaa
     1561 acagagccat ttttttgccc atctctcccg cctgaaactc attaaccgct ggccgctcat
     1621 gcgcaacgtg cggacggaaa atgtgtccga acacagtttg caggtagcga tggtcgccca
     1681 tgcgctggca gctatcaaaa atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc
     1741 tttactggcg atgtaccacg atgcctcaga agtgctcacc ggcgatctcc ctactccggt
     1801 gaaatacttc aattcgcaaa tcgctcagga gtacaaggct attgaaaaaa tcgctcagca
     1861 aaaactggtc gatatggttc cggaagagct gcgggatatc tttgcgccgt taattgacga
     1921 gcatgcatat agcgatgaag aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata
     1981 tctgaaatgt ctggaagaac tcgcggccgg aaataatgaa ttcttgctgg caaaaacgcg
     2041 actggaagcg acgcttgaag cgcgtcgcag ccaggagatg gactacttca tggaagtatt
     2101 tgttcccagc ttccatcttt cgctcgatga gattagccag gattcaccgc tgtaagcagc
     2161 cggagtctgc gtcgcatcag gcaataagcg ccggatgcga catcaggctc ttgtcaaaac
     2221 ggaaacagca tcgggatcat caccacacaa accgccatca cgataatggt gaacggtacc
     2281 cccaacttca caaagtcact gaagctgtaa tttcccggac ctaaaaccag tgtgttaaca
     2341 ggtgaagaaa ccggtgtcat aaaggcggcg gatgctgcca tcgccacgac catcgcgaat
     2401 ggataaggcg acacccccat cgttttggca gcagccagcg caatcggagc catcaacacc
     2461 gccgtcgcgg tattagagat aaatagccca ataaccgccg acaagacaaa caaacaaccc
     2521 agcatcatat gtggcccgta accaccgcca atgtccatca gccctttcac cgccagcgcg
     2581 acacctcccg ttttctgtaa tgccacagca aacggcatca tcccaacgat caaaataatg
     2641 ctcggccagt gaatggattt ataggcgctt tcagcatcta tacagcggaa tttccccatc
     2701 agcaggcagg cgatgatagc ggcaacagga ttaggaattt catctgtcag cattaacgcc
     2761 accattagca ccagacagaa aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca
     2821 ctcacctctt ccggtaagtt cagcgctacg aagtcgcggc cctgtttggc cagcataccg
     2881 atcagtttcc agttacccac aaccaggatg atatcgccca gcagcagagg ctcatccgcc
     2941 agcgaacctt ccagcgccac gccattgcgc tttagcccca ccacattcag tccgtagcgg
     3001 gtacgaaaac caatttcgcg caccgattta ccaatcagtt ctgactcagg aattaatgaa
     3061 atctctgcca tgcccacatc aagggcctgg tcagaaaaat actcgccgcg cagtaccatc
     3121 ggctccagca attgctcact acaaaattgc cggagatcga catcagccgc agacatatca
     3181 ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat taacgttcac gataacgcga
     3241 cgaaaacgcc gccagcgttc aacaccgatg acgttagcgc cataacgctc acgtaatttg
     3301 agatcatcca gccgttgacc tatcattggc gatccggggc gaatagccag acgtcgcgca
     3361 cgcccggtca gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg
     3421 cgctgcgggg tctgggtatc ccctttcagc atgaaacgca tcactaacat atacaaaata
     3481 cccagcacca gcacgaccag gccaataggt gttacgctaa agaaactgaa gccgtgatag
     3541 ccttcacgca gcaattcact gttgactacc aggttcggcg gcgtcgccac cagcgtcatc
     3601 atgccgctaa tcagcccggc aaaacttaac ggcatcatca gacgtgacgg cgaagtttgc
     3661 atacgcatgg caacgcttaa caccacggga ataaagatag cgacaacgcc ggttgaactc
     3721 ataaacgcgc caagccccgc gacggtcagc atcaacaaaa ccaacatttt gatttcactg
     3781 ttgcccgcca ctttgaccag ccatgttccc attacggtgg caacaccggt acggaccaaa
     3841 ccatcgccaa taataaacaa ggcggcaatc aggacaacgt taggatcaga aaagccggaa
     3901 aatacttcgg ggaccgtcag cgttccgctt aatgcaaacg cgacaataac aaacaaagcg
     3961 accgcatcca tacgcactct gcccgtcgca aacaagacga tggcaacggc cagtaatgaa
     4021 agaacccaaa tcaattcacc gttcacaact tatccttgtt aattgagggg gatgacttga
     4081 ttctgccata aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg tttcactgaa
     4141 taataacatc gccatttggc tgtttggtca cagtaatttg ctccagactg tggaggacca
     4201 aatcgacctc attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg acatggcaac
     4261 ccgccgccag gccagaaagc acgccagcgg gagcatcttc caccaccaca cactcctgcg
     4321 gcgcaagccc cagcagctgc gcacctaaca gatacgcatc aggttctggt tttccgcgct
     4381 tcactcgctc agcggttaca aacacctctg gtgcgggaag cccagctatt ttatggcgcg
     4441 ctcgcgctac cggcatggag ccagaagtca caatggccca cggaatacct gctttattca
     4501 aatgattgag taaggcgatt gcccccggaa gcgcggtaat accttcggtt tccgtggcct
     4561 cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg cccgccataa
     4621 aatggcgcaa agaggtgatc gcctgtttac cgtgaatgaa agccagcacc tcttccggcg
     4681 ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc agggaatcca
     4741 ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc tcctcaggcg
     4801 ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca ggcatgccac
     4861 acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa accgtgagtt
     4921 ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac ataaccgagg
     4981 taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc atcgatggat
     5041 tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc cataatgtcg
     5101 atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa ttcacgcatc
     5161 tgtaatccgt aaccacgctc aataattgtt tgcaggcgac ggtaacgttc agcatttgcc
     5221 ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg cgcgttggtc
     5281 atttccattg ttgactcctg tatcactata ctacggtgaa aaaaaagaag gctgagtatg
     5341 ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag ttttatccag
     5401 ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct tgccttcaac
     5461 gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa accagttaag
     5521 gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc cctgcatggg
     5581 taaacttaag gcgaacagcg cagtggcaac tgccggccca agctgaccgc ccagggcaat
     5641 ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat aacgcgtcat
     5701 cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagcg gccacgtctt
     5761 tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta acagaacgat tatccggcgt
     5821 tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa attcacaact
     5881 cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta tcctttagca
     5941 gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac attatgccat
     6001 tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca tgcaaaaaaa
     6061 atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac gctctatggc
     6121 tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga gtaagttagt
     6181 actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg cagtaaatgg
     6241 tgaagagtac ctttctggtt tagccgaatg tttccacctg cccgaagcac gtatcaaatg
     6301 gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc acagcgaagc
     6361 gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg cgcagctgac
     6421 tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg tagtgatcga
     6481 tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc acaacccggc
     6541 tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag acaaaaacgt
     6601 tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc tctacgccct
     6661 gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg gcaccagcca
     6721 cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag aactgaacat
     6781 catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg gtaaatgcgt
     6841 tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc gttctggtga
     6901 tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg ttgacgcaat
     6961 caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga ccagcgactg
     7021 ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa tggacgttta
     7081 ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg gtcgtctgga
     7141 cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg aactgtctct
     7201 gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg ctgcacgttt
     7261 cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta tcccaaccaa
     7321 cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc acaccgccag
     7381 ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag aggataaacc
     7441 gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac cagcgtcagc
     7501 cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt caaacctatc
     7561 gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt gcgtgcgaac
     7621 tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg tctgctttcc
     7681 agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc taacaccaaa
     7741 gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca gtttgcccag
     7801 tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt tatgtctcag
     7861 ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa cagcttcggc
     7921 ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc accggttgat
     7981 gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc caaagctaaa
     8041 gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc cgctgccggt tctcggcgct
     8101 gtgccgtgga gctttgacct gatcgcaact cgtgcgatcg atatggctcg ccacctgaat
     8161 gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt cactttctgc
     8221 gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct ggtgacttcc
     8281 gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg cgtagaaatc
     8341 ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa actgtgcgaa
     8401 cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg gcagacctct
     8461 ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgagcg tatcgagaaa
     8521 gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct gactgccact
     8581 tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac tgaacttgcg
     8641 cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac cgttaaagca
     8701 gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa tccggcagag
     8761 atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga aatcgttgat
     8821 ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa gaacaaaggc
     8881 atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg tacgctgatg
     8941 ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac cgcaaacacc
     9001 atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt atcttccgtg
     9061 ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat caacccggat
     9121 ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc tgcggccttc
     9181 ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg tgcaggtagc
     9241 gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc tgacctgatg
     9301 atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa atccaaagcg
     9361 ccgaactctc cggttgcagg tcgcgctacc gtgttcatct tcccggatct gaacaccggt
     9421 aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg gccgatgctg
     9481 cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga cgatatcgtc
     9541 tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaatctcg tcatcatccg
     9601 cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt ttttattctc
     9661 ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca atgaattgcg
     9721 ccatttcact ttgcatactt accactttgt tttgtgcaag ggaatatttg cgctatgtcc
     9781 gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt ggtgattatc
     9841 ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg gatgtttgac
     9901 agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt cgtagatcca
     9961 cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg cgtacagctg
    10021 ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg attaacctca
    10081 ggatcgaaat acgggacagc cgttggcatc atcatgttta tgctggtgat tggcggcgcg
    10141 tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct tattcgccat
    10201 acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc acttggcggc
    10261 gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc accgctaatg
    10321 gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc cacgcaaatc
    10381 ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg gattgccggc
    10441 gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc cactatgatt
    10501 ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct tctgtcacgc
    10561 gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca acgtccgttt
    10621 acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg ggtgatttgg
    10681 ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt caccatgggt
    10741 ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt taataccatg
    10801 gcttcatcct ttaccgaagg ggcgcgaatg atgatcgccc ctgccctgct ggtgggtttc
    10861 gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag cgtgttaaat
    10921 accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt cgcggcctgg
    10981 tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc tggtcaggcg
    11041 gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa ccgtcaggtt
    11101 accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc aacctcagct
    11161 tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct gaaggtgggc
    11221 gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg cgctcagttg
    11281 atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca ttctgccgtt
    11341 tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag cgctttcagc
    11401 gagtctggag tgaactcatc gcagcgcgcg gtgatttctt ccggcgtcag ccagcaaact
    11461 tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcaact gaacaacgcg
    11521 ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc aaaggggaca
    11581 ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag ttgctcatcg
    11641 gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc ttttgtctcg
    11701 gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta agttgcacga
    11761 tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac ttcgttctct
    11821 tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat tatcaggaaa
    11881 ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta cggattaatc
    11941 gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac gtcattatcc
    12001 agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc ggggttgaag
    12061 tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta caccagcaca
    12121 tcgttctggt ttaaagcagg cagattttcc gggccaaaaa gatgaccatg cgtcaaaaac
    12181 agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa atgcagcagc
    12241 atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg tgccacttca
    12301 ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt acgcgggccg
    12361 tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc aaacaactcc
    12421 agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat cagtttcatc
    12481 actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt gctatcagcc
    12541 agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc actggaatgt
    12601 cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc ggttaatcat
    12661 tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct gacccagtgc
    12721 taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct cggccgttag
    12781 tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc ggatgggcat
    12841 cagatcgctg cgcagccagg cctgaatctg tcgtgcacgc gcacgatttt ctaaatcaag
    12901 cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact ccgcaatggc
    12961 agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc gtgtctgacc
    13021 gtaaccttgc cacgtcggct gcaaatgttc accgctgtcg aggtcgatgg tcttgatatg
    13081 aaacgacagg cctttttcct gcaacgccac ccaggcagat aacacataag gggagaaaaa
    13141 gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg caataaaggg
    13201 ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata ctcacgtaag
    13261 tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc tgcaatgatc
    13321 gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct cgaagaagca
    13381 gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt tcgtccggaa
    13441 tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc tcctgccgat
    13501 ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct ggctgagaaa
    13561 acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca gtggttattc
    13621 tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa tcacgcagcc
    13681 ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg tctttaccat
    13741 gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta cagcattgcg
    13801 gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga cctcgcaatg
    13861 tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac cgggcaggca
    13921 ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca ggttctcgtg
    13981 tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct gccgctatta
    14041 ttcgtataaa gaaccttcgt ttacgcacgt ttattggaat taaggacgaa gaaattaaca
    14101 accgtcagga tattgttatc aatgttacga tccactaccc cgccgataaa gcgcgcacca
    14161 gcgaagatat taatgacgcg ctgaactatc gcaccgtcac caaaaacatt attcagcatg
    14221 tagagaataa ccgtttctct ttgctggaaa aattaactca ggatgtgctc gatatcgcac
    14281 gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactacac gcgctgcgct
    14341 acgccgattc ggtatccatg accttgagct ggcagcgtta accgccatat cgggaggctg
    14401 catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga ttccgcgttt
    14461 gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag ccagttccgt
    14521 tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc tcaacggcgt
    14581 tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga ctcacgagca
    14641 aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg atttgattaa
    14701 tgccagcgac acgccgccgt cggtactcat ttccggctcg gcaacgggct attatggcga
    14761 cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta ctcataaact
    14821 ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc gagtgtgcct
    14881 gctgcgtacc ggcgtagtgc tggcaccgga tggcggtatt ctcggtaaaa tgctgccgcc
    14941 gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg cctggattca
    15001 tatcgatgat atggtcaacg gcattctctg gctgctggat aacgagctgc gcgggccatt
    15061 taatatggtt tcgccctacc cgatacgcaa tgaacaattt gcccatgcgc tcggtcatgc
    15121 gctgcatcgc ccggctattt tgcgcgtccc cgcaaccgcc attcggctgt taatgggcga
    15181 atcttcagta ctggtattag gtggacaacg cgcgctgcct aaacggctgg aagaagcggg
    15241 ttttgcgttt cgttggtacg atttagaaga ggcgctggcg gatgtcgttc gctgatgtgg
    15301 tttactgcaa acatccgcca gtta
//
LOCUS       DAEVZA010000116        15284 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEVZA010000116 DAEVZA010000000
VERSION     DAEVZA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.116"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1277)
                     /locus_tag="LAF92_003757"
     CDS             complement(<1..1277)
                     /locus_tag="LAF92_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBI9792691.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATA"
     gene            complement(1390..3324)
                     /gene="yeaG"
                     /locus_tag="LAF92_003758"
     CDS             complement(1390..3324)
                     /gene="yeaG"
                     /locus_tag="LAF92_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBI9792692.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3760..4506
                     /gene="mipA"
                     /locus_tag="LAF92_003759"
     CDS             3760..4506
                     /gene="mipA"
                     /locus_tag="LAF92_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBI9792693.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4596..5450
                     /gene="yeaE"
                     /locus_tag="LAF92_003760"
     CDS             4596..5450
                     /gene="yeaE"
                     /locus_tag="LAF92_003760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBI9792694.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5501..6385)
                     /locus_tag="LAF92_003761"
     CDS             complement(5501..6385)
                     /locus_tag="LAF92_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBI9792695.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6469..7464)
                     /gene="gapA"
                     /locus_tag="LAF92_003762"
     CDS             complement(6469..7464)
                     /gene="gapA"
                     /locus_tag="LAF92_003762"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBI9792696.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7806..8219
                     /gene="msrB"
                     /locus_tag="LAF92_003763"
     CDS             7806..8219
                     /gene="msrB"
                     /locus_tag="LAF92_003763"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBI9792697.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8261..8533
                     /locus_tag="LAF92_003764"
     CDS             8261..8533
                     /locus_tag="LAF92_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBI9792698.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8694..9335)
                     /gene="pncA"
                     /locus_tag="LAF92_003765"
     CDS             complement(8694..9335)
                     /gene="pncA"
                     /locus_tag="LAF92_003765"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBI9792699.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(9346..10362)
                     /gene="ansA"
                     /locus_tag="LAF92_003766"
     CDS             complement(9346..10362)
                     /gene="ansA"
                     /locus_tag="LAF92_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBI9792700.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(10529..12385)
                     /gene="sppA"
                     /locus_tag="LAF92_003767"
     CDS             complement(10529..12385)
                     /gene="sppA"
                     /locus_tag="LAF92_003767"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBI9792701.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRLIPAQQVFPGAQGLLVGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            12546..13097
                     /locus_tag="LAF92_003768"
     CDS             12546..13097
                     /locus_tag="LAF92_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBI9792702.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            13214..14257
                     /gene="selD"
                     /locus_tag="LAF92_003769"
     CDS             13214..14257
                     /gene="selD"
                     /locus_tag="LAF92_003769"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBI9792703.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            14262..>15284
                     /gene="topB"
                     /locus_tag="LAF92_003770"
     CDS             14262..>15284
                     /gene="topB"
                     /locus_tag="LAF92_003770"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBI9792704.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHF"
BASE COUNT         3771 a         3796 c         3933 g         3784 t
ORIGIN      
        1 ttagctgttg cattttgttt atgaaacagt tcacggaaca ccggataaat atcatcctgg
       61 tcgcggatgt gctgcatcgc aaagttgtcg aaagtagatt gcagatgctc atattctcgc
      121 cacaatgtct gatgtgcacg acgggtaatt tcgatatagc tgtaataacg aacaacaggt
      181 aataattttt tcgccaggat ttcatggcaa agcggagagt catcggccca gttatcgccg
      241 tccgatgctt gtgcagcgta aatattccac tgtgccgggt tataacgctc tttcactacc
      301 tcatccatca gtttcagggc gctggaaacg atggtgccgc ctgtttcctg cgagtagaaa
      361 aactcatgtt catcgacttc tttcgcctgg gtatgatggc ggatgtatac gacttccacg
      421 ttcttatacg ttctgctgag gaacagatac agcagaatat aaaaacgctt agccatatct
      481 ttagtggatt gatccattga accggaaacg tccatcaggc aaaacatcac tgcctggctg
      541 gagggatcgg gccgcttctc gtagttcttg taacgtaaat cgaaggtgtc aataaaaggg
      601 acgcgttcaa ttttggcacg taattctgca atttctttgc gcagacgttc ctcttccagc
      661 agctgagcag gttcactgtt gctgatgatg gccaaattct cttccagtgc atgaagttcc
      721 cgccgcttgc ctgccgtcat ggctgtgcgt cgcgccagtg agttctgcaa ggagcgcaca
      781 acgctgatat ttgccggaac gccgttagcg gtataacccg cccgatgcgt tttatattcg
      841 gtcagctggc gttgttggtt ttgtttcaga ttcggtaagg ccaaatcttc aaagagcaaa
      901 tcaagatact catctttcga aatctgaaag acaaattcat cctgaccttc accatcctgg
      961 ctggcctggc cctgaccact gccggaacct ccgccgccac cctggggacg ttcaattcgg
     1021 tcgttctgga cgaaatggtc attgcccgga tgcacgcggt ggcgcagacc gccacgcccc
     1081 tgatgaaaca tcggttcgct aatatcttcc gtgggaatgg atacggattc gccgctgtcg
     1141 acgtcagtca ccgaacgctt attaatggcc tcggagatcg actgtttaat ttgcgcttta
     1201 taacggcgta aaaaacgctg gcgattcacc atgcttttgt ttttgccgtt cagacgccgg
     1261 tcaataaacc aggtcatatg ccccccgtac tacatttgcc aactgtatgc tacgcctgtg
     1321 ttgccgcttc tcaggcccac ggttcgctgt cgttgtaggc ccggtaacat gtgtaccggg
     1381 ccagggttgt taagacgatt tacgtacgcg caaataccat tcgcacagta aacgcacctg
     1441 tttacgggtg tagccttttt ccatcatacg gtcgacaaag tcgtcgtgtt tcttctgctc
     1501 gtcggttgac gttttggcgt taaacgagat aaccggcaac agctcctcgg tattggagaa
     1561 cattttcttc tcgataaccg tgcgcagttt ttcatagctg gtccagttcg gattgcgtcc
     1621 gctgttattc gctctggcgc gcagtacgaa gttaacaatc tcgttgcgga aatcttttgg
     1681 attactgatc cccgccggtt tctcgatttt ctccagctcg gcgttaagag actcgcggtc
     1741 aaacagctgc ccggtatccg gatcgcgata ctcctgatcc tgaatccaga aatccgcgta
     1801 ggtaacataa cggtcgaaaa tgttttgccc atattcggaa taggattcaa ggtaggccgt
     1861 ctggatctct ttgccgataa actcggcata tttcgggatc agataacctt tcaggaactc
     1921 cagatagcgt tctgcctgct cttgtgggaa ctgctcgcgc tcgatctgct gttccaggac
     1981 gtagaacaga tggaccgggt ttgccgccac ttctacatga tcgaagttga acacgcggga
     2041 gaggatctta aacgcaaaac gcgtcgacag accgttcatc ccttcatcga caccggcgta
     2101 gtcacgatat tcctgatacg acttggcttt gggatcggtg tctttcagac tttcgccatc
     2161 ataaacccgc atctttgaat aaatgctgga gttttctggc tctttaaggc gcgaaagaat
     2221 ggaaaaacgt gacagcgttt ccagcgtgcc aggggcgcag ggggcgtgag tcaattcact
     2281 gtgattaagc aatttctcgt agattttgat ctcttcggaa atgcgtaagc aatacggcac
     2341 cttcacgatg taaacacggt cgaggaaggc ttcgttgttt ttgttattac ggaaagtgac
     2401 ccattcggat tcgttcgagt gggcgagaat aatcccgttg aacggcaggg cggagatacc
     2461 ttccgtcccg ttgtagttac cttcctgagt ggcggttaac agcggatgca gcacttttat
     2521 cggtgcttta aacatctcga caaattccat gatcccttgg ttggcgcggc acagcgcacc
     2581 ggaatagccg taggcgtccg ggtcattctg cgcgtagtgt tcgagtttac gaatatcgac
     2641 tttcccaacc agcgcggaga tgtcctggtt gttctcatca ccgggttccg ttttggcgat
     2701 agcaatttgt tgcagaattg acggccagac cttcactacc cggaacttag tgatatcgcc
     2761 accaaattca tgcaggcgtt ttgccgccca tggcgacatg atggtgccga gataacggcg
     2821 aggaatgcca tactcttttt ccagaatctg agcatcttcc tgcggattga aaagacagaa
     2881 cggatgatcg ttgaccgggc tacgctcacc gttggcgctc aatacataaa tcggtacgag
     2941 ctgcattaat gatttcagtc gctcagcaag cgatgattta cccccaccca caggccccag
     3001 cagatacagg atttgtttct tctcttccag cccctgagcc gcgtgtttca gataagagac
     3061 aatctgttca atcgcgtctt ccatgccgta aaactcttca aatgccggat aacgtgcaat
     3121 gacccggtta gaaaagagtc gagaaagtct gggttcctgg gctgtatcga ccatgacagg
     3181 ctcaccgata gccatcaata gccgctcagc cgcgttggca taagcactgc gatcttgccg
     3241 acaagtggta agaaactcct gcagtgtgaa ctcttcgtcc ttggcagctt catatcgctg
     3301 gcgatagtga tcgaatatat tcatggtatg ccgtcctttc gttttttagc acaggttaag
     3361 agccgttcat atgaatagta gaggctcccg gaagaggtaa actgaacgac gtctcttgct
     3421 aaaacacagc aactctcatg ccattttatc gacgatgaga acaacgtgcc cggtgataca
     3481 ccttcaaaat taagcgtaga tggcaaatgg aaaacttgcc tgcacggctt tcttaatttc
     3541 aatggcatat caacaactca tccataaatt ttgcataatt aatgtaaaga ccagggtagc
     3601 cagtaacgct aaattcattt ggctgtaagc gcggtgtcat ccgcgtcagg aaaattaaac
     3661 agttacttta aaaaatgaaa acgtaaaaag gttgggtttc gatgtattga cgggtaaact
     3721 ttgtcgcccg ctaaacattt gtttaaggaa tgattaattg tgaccaaact caaacttctg
     3781 gcacttggag tgcttatcgc aacgtctgca ggcgtagcgc acgctgaagg taaattttcc
     3841 ctgggcgcag gcgtaggtgt cgttgagcac ccatataaag attacgatac cgatgtttac
     3901 ccagtaccgg taatcaacta tgaaggcgat aacttctggt tccgtggctt aggtggtggt
     3961 tactacctgt ggaatgacgc aacggataaa ctttcaatta ccgcttactg gtcgccgctt
     4021 tacttcaaag cgaaagacag tggcgatcac caaatgcgtc acctggatga ccgtaagagc
     4081 accatgatgg ctggtctgtc ttatgctcac tttacccagt acggttacct gcgtaccacc
     4141 ctggctggcg ataccctgga taacagcaac ggcattgtct gggatatggc ctggttgtat
     4201 cgttacacca acggtggcct gaccgtgact ccgggtattg gtgtgcagtg gaacagcgaa
     4261 aaccagaacg aatactatta tggcgtatcg cgcaaagagt ccgctcgcag cggtctgcgt
     4321 ggctataacc cgaacgacag ctggagccct tacctggagc tgagcgccag ctacaacttc
     4381 ctcggcgact ggagtgttta cggtaccgcg cgctataccc gtctgtctga tgaagttact
     4441 gacagcccga tggtggataa atcctggact ggcctgattt ctaccgggat cacctacaaa
     4501 ttctgataat gcatctttcc agggcatttt gtgcataaaa acagggcgtc acgccctgtt
     4561 ttgcattacg gtggtgcaat acctgggagg aaacaatgca acaaaaaatg attcaattta
     4621 gtggcgatgt ctcactgcca gccatagggc agggaacatg gtatatgggc gaagatgcca
     4681 gtcagcgcaa aacagaagtt gctgcactac gcgcgggcat tgagctcggt ttaaccctca
     4741 ttgataccgc cgaaatgtat gccgatggcg gtgccgaaaa ggtggttggg gaagcattaa
     4801 ccggtctgcg cgataacgtc tttctcgtct ctaaagtcta tccgtggaat gctggcgggc
     4861 aaaaagcgat aaatgcatgc gaagccagtt tacgccgtct caatactgat tatctcgatc
     4921 tctacttatt acactggtct ggcagtttcg cttttgaaga gactgtggca gcgatggaaa
     4981 aattgatcgc ccagggaaaa atccgccgct ggggcgtttc taaccttgat catgctgata
     5041 tgcaggaact ctggcagctg ccggggggaa atcagtgtgc cactaatcag gtgctttacc
     5101 atctcggttc acgagggatt gagtacgatc tactcccctg gtgccagcaa cagcagatgc
     5161 cggtgatggc ttacagtcca ttagcccagg ccgggcggtt gcgcaatgga ctgttaaaaa
     5221 acgcggtagt caacgaaatt gcacatgctc acaatatcag cgcggcacaa gtattgttgg
     5281 cgtgggtaat cagccatcag ggtgtgatgg cgattccaaa agcggccacg attgcccatg
     5341 tccaacagaa tgcggctgtg cttgaggtcg aactttcgtc agcggaatta accatgctgg
     5401 ataaagcgta tccggcacca aaaggaaaaa ctgcgctgga tatggtgtga tgttgatacg
     5461 tgccggatgc atcgcacccg gcacacaaca ttaacgcaaa ttaacgtttc gcaacgcgaa
     5521 tggattgcgc cagatgtgca ggtttctctt tggtcacttt ttgcgtttct gaagcgtaag
     5581 ccgtttctac acaaacaaat gttttgtagc catcatccgg catgtcgccc atgctaactg
     5641 aaagcgccgg tcccgggttc cagccgacaa cgttcagatg atgctggtgg cctacggcga
     5701 taatacgatt cagcgcttca tcattaatca cgctgcaatc ttgcggattc agatacacgc
     5761 gatcggtacg gtcagggaag gtctgaatac cgtcggtcag tacatcttct ttcgcgtcat
     5821 ttactttatc aatgaagcga tcgcccagcc cactgacgct taccttagcg atatcaccca
     5881 cgttaaagta ggtatgcagg gcagaggtgg tttcaaattc gccatgtgat tcaagatcga
     5941 tttcacaggt tttacccacg cggaaatgcg ctaacagcgt aaagtcgtgc ggccagaatt
     6001 ttttcgtctc ttcgctttgc gtcaattcaa aagtcagcgc tacgccatca gcatcttcac
     6061 gatgtgattt cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc agaccttgtt
     6121 gtgccgccgg accaaaccac ggccagcaaa ccggtacgcc accgcgaata gcgacgccat
     6181 ttttgaacgg tgtgttgttg ctcaaccaca gaacttcttc ttcacccgca ggtttccacg
     6241 agagaaggtg tgcgccctgt aatgcaaaag aggcttttac ctggggatga tcgaccacaa
     6301 tgaggtccag ttcatccagt ttacgacggg agaggacagg ggagatttgt tcgatgaccg
     6361 gaagggcaaa aattttctta atcatgacgc agtcctttaa cttcatttta tcaggtaaaa
     6421 aaaagagcga ccgaagtcgc tctttttaga tcacagtgtc atctcaactt atttggagat
     6481 gtgagcgatc aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt accaggatac
     6541 cagtttcacg aagttgtcgt tcagagcgat accagcttta gcatcgaaca cggaagtgca
     6601 aacttcgccg ttgaaatcgg tagatactac gtcatcttcg gtgtagccca gaacgccttt
     6661 catttcgcct tcagcagcag ctttaacggc agctttgatc tgctcgtaag ttgcagcttt
     6721 ttccagacga acggtcaggt caactacgga tacgttcggg gtcggaacgc ggaacgccat
     6781 accagtcagt ttgccattca gttctggcag tactttacct acagctttag cagcaccggt
     6841 agaggacggg atgatgttct gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg
     6901 gccatcaacg gttttctgag tagcggtagt agcgtgaacg gtggtcatca gaccttcgat
     6961 gatgccgaag ttatcgttga taactttagc cagcggagcc aggcagttgg tggtgcagga
     7021 agcgttggaa acgatgtcct ggccagcata tttgtcgaag ttagcgcctt taacgaacat
     7081 cggagtgttg tctttagacg gaccagtcat aaccactttc ttcgcaccag cggtgatgtg
     7141 tttacgagca gtttcgtcag tcaggaacag accagttgct tcagcgacaa cgtcaacacc
     7201 aacttcgtcc catttcaggt tagccggatc acgttcagcg gtaacacgga tttttttacc
     7261 gttaacgatc agatgaccgt ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga
     7321 gtcatatttc agcatgtatg ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat
     7381 ctcgatgtca gaacgtttct gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc
     7441 gttgatacct actttgatag tcatatattc caccagctat ttgttagtga ataaaaggtt
     7501 gcctgtaaaa ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac
     7561 aaatcaatcc tgtgcctaag cattacgcga ctgactcgcc tcactcttcc tttgggcttg
     7621 agaccacatg gggtcggcgc cccgaatttt aaagggcaat tacgataaaa atgtgatttt
     7681 catcacgatt taggtgcagc catttcgtcg agatcaagtt taggacaaaa ttttgccagt
     7741 tggtgttaat gttttgttag aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg
     7801 agcaaatggc taataaacct tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt
     7861 tttacgtgac gcagaatcat gggacagaac cgccatttac gggtcgttta ctgcataaca
     7921 agcgtgacgg cgtatatcac tgtttgatct gcgatgcccc gctgtttcat tccgaaacga
     7981 agtatgattc cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa gaatccattc
     8041 gttatatcaa agacttgtca catggaatgc agcgcataga aattcgttgc ggtaactgtg
     8101 atgcccatct ggggcatgtc tttcccgacg ggccgcaacc aacaggcgaa cgttattgtg
     8161 ttaactctgc ttctttacgc ttcactgatg gcgaaaacgg tgaagaaatc aacggttgaa
     8221 aaaacgattc agcaacttat tccacaggag cggaaaaatt atgaatcttg atgacattat
     8281 caacagcatg acgcctgagg tataccagcg tttgtcgacc gccgttgaac tggggaaatg
     8341 gcctgatggc gttgcgttga ccgaggaaca aaaagagaat tgcctgcaac tggtgatgct
     8401 gtggcaagcc cgccataata ccgaagcaca gcatatgaca attgacacaa atggtcagat
     8461 ggtcatgaag agcaaacagc agttaaaaga agattttggt atctcagcaa agcctattgc
     8521 tatgtttaag tgaatgagtt atctagtatt taagcctgtt attaagggag ggcaaatata
     8581 cttgttagga cgtcgtgttt tagctgcaag taggttaaat gcaacactga caagtattat
     8641 ctggcctaca atgtcattga ggaaatgtag gccagagatg aattcaacta atattacccc
     8701 tgcgtctctt cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata
     8761 aacgcgtgcg cactgtcctg gggctggata ttcacgccac gacaaccatc ggtaatcacg
     8821 tttaccttat aacctaactg taacgcgtcc agcacggtaa acttcacgca atagtcagta
     8881 gccaggccca taacgatcaa ttcatcgatt tcatgatcgc gtaaccagtc atcgagagag
     8941 gttttctgcc gacggccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg
     9001 cctttatgga acaccgctgc gatcgctttt tggttcagta acggatgtaa ttgtgcgcct
     9061 tcactgttct gcacacagtg atctggccag aaggtttgtg gcaaaccgtc gagttgacct
     9121 ggcatataag gctctacaca gtgctgactg gcaaaactgc cgtgattcgc cgggtgccag
     9181 tcctgactgg cgataaccgc ttcaccgcgc gactggcacc agtcaataag gcggttagcg
     9241 acatccaccg tactgtcacc ttccggcacg gcgagcgcgc caccagcaca gaaatcattt
     9301 tgtaaatcga ccagtaacag ggcgcgaggg ggcattgcag tctccttaat catccggcgt
     9361 cagttcgccg cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg
     9421 gctcagcagg taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgccaat
     9481 aacgccggca tgggcgaggg cgttaccggt ggcataacca cccatattca ctttaccgga
     9541 catacattgt gtcaggttga ccaccacaat accgcgatcg ctggcttctt gtaattcctg
     9601 caggaaggct ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt
     9661 caccggttgg cgcagaaaat tgcgcacgac atcagcagaa atccccggat aaatcgtcac
     9721 tacgccaatt ggttgtggcg tgattggatg aacgattaat tccccttcac cgtgcggggc
     9781 gggtggcgta ttcaaacgac gaatatggat accagcttcc agtaacggag gaaggtttgg
     9841 agaggcaaac gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacaa
     9901 tcggttattg aaaaagagcg ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc
     9961 attcagcaga ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt
    10021 cacaatgacc ggtttaccga gattctcgag catgaacgac agcgcagagg cggtatacgc
    10081 catcgtgtcg gtaccgtgta gaatgacaaa accatcatag tcgtcatagt gcgctttaat
    10141 atcttcagca atatgctgcc agtcttccgg cgtcatatca gaagaatcca tcaacggcgt
    10201 atattcatga atggtgaaat ctggcatctc cgggcgatgg aattccggca tcagcgccag
    10261 ttggcgttgt agatgacctg acaccggaat ataaccctgc tcggaacgct gcatcccgat
    10321 ggtcccgccc gtgtaggcga cgtaaattga tttcttttgc atgatattga tatgaatgat
    10381 atcgaaagag caaaagtata tacgtgaggc aggaggagcg cacctgatac tatgcggata
    10441 aggaaatatg ggtcgagaca gcgggaagag gattcgtcga ggtaaaaaat aatgtggccg
    10501 acgatctgtc ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg
    10561 cataacggtt ttgtgggtcg ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag
    10621 aggctaccga ggcaagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc
    10681 ggacagaacc agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt
    10741 attccagatg ccactgtttc acttttgcca gctctgctgc tttggcaacc gcatcatcga
    10801 aatccccgag actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacgtggc
    10861 cctgggcgat tttatcaatt tgctccggcg tcgaatgacg cgcatcagca accagcgtga
    10921 taaagcgttt atagccattc tcaatgctta attgcatcat ctgctgcgct tccggcggca
    10981 gtgccctggt gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac
    11041 caatcgaatc cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg
    11101 tcagggtgct ggggttagcc acaatgtaat tagctggcgt ggaaatccag taaccaccag
    11161 atgccgccat gccgcccatc gatacaacca caggcttacc cgctgcccgg gctgctgcca
    11221 gttcagcgcg aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga
    11281 caatcgcttt cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac
    11341 cgccaacatt cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgacac
    11401 cgatgctgtc accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc
    11461 gataattttt atcagtctta ctccagccga actctttggt cagagctttt tcgatttcgg
    11521 cactcgatgc cagtgcatcg accagcttgt tttccagtgc atatttcgcg gtatcgccac
    11581 cggttttggt taaacccaca agcaaccctt gcgcgccagg gaatacctgc tgagcaggga
    11641 tcagccggtt agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc
    11701 tgtcagcttc gcgcgctgcc ggtgacatat catcacgaat aaacggttca acggcagatt
    11761 tatacgtacc cacgcggaac acatgggtgg aaactttcag cttatccagc aacgatttgt
    11821 agtacagacc gttggtagca aaaccgtgca gatcaaccac gccttgcggt gacagccaaa
    11881 ttttattggc gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat
    11941 aaaccggttt cccgctgtca cgaaactctt tcagggcttt gccgatgtac tgcatagacg
    12001 gctggtcgcc gcctgcgaag tttttcagat ccatcacgat accggtgata ttgcggtcgt
    12061 ccttcgcctg gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg
    12121 aactggcacc aagcagctgg cggcttaatt tactaaaccg ctgagaactg tcgggtttat
    12181 cgacgatcac accagaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat
    12241 caccaccact gacctgcatc cagatcccca caccaaccag tacgaggaaa ataaagaaca
    12301 ggttaagtac catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg
    12361 caataaatcg ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg
    12421 cgcaatagat gtggttatcg taatgaccca accggcactt gtcagcagga attgcggact
    12481 gtgctgtaac aaaatccact ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta
    12541 atcaaatgga tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ctggctgaac
    12601 ccgcgccaac gggtgaacaa ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc
    12661 ataagtccat gcaaccgtgg catttttttg tgattgaagg ggaagggcgc gagcgtttca
    12721 gcgccgtact ggaacagggg gcgattgctg ccggtagtga tgacaaagct atcgacaaag
    12781 cccgtaatgc gccgttccgc gcaccgctca tcatcacggt ggtggcgaaa tgcgaagaaa
    12841 atcataaagt cccgcgatgg gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc
    12901 aaatggcagc cgttgcccaa ggatttggcg gcatctggcg cagtggcgca ttaactgaaa
    12961 gtccggtagt gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct
    13021 acctcggcac gccgcagttg aaagcatcta cgtcgattaa cgtcccggac ccgactccgt
    13081 ttgtaactta tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga
    13141 attcagacac tctcacttat cacttcacgg aatgagggtt accatacccg gattgcattg
    13201 acaggagatg tccatgagcg agaactcgat tcgtttgacc caatacagcc acggagctgg
    13261 ttgcggctgt aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa
    13321 gtttgttgat ccgaatttgc ttgtgggtaa tgagacccgc gacgatgcgg cggtgtacga
    13381 tctgggcaat ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa
    13441 tcctttcgat tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcgatggg
    13501 gggcaaaccg attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga
    13561 aattgcccgc gaagtaaccg aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct
    13621 ggctggcggt cactccatcg atgcgccgga gccgattttt ggtctggcgg taacggggat
    13681 cgtaccgacc gagcgggtga agaaaaacag taccgcacaa gccggatgca aactgttcct
    13741 gacgaaaccg ctggggatcg gcgttcttac cacggctgag aaaaaatcac tgttgaaacc
    13801 agaacatcag ggactggcga cggaagtgat gtgccggatg aacatcgcag gtgcgtcctt
    13861 tgccaacatc gaaggcgtaa aagcgatgac cgacgttact ggttttggtc tgctgggcca
    13921 tttgagcgaa atgtgtcagg gcgcaggtgt gcaggcgcgc gttgactatg acgcgatccc
    13981 aaaattgccg ggggttgaag agtacattaa gttgggcgca gtgcctggcg gcaccgaacg
    14041 taactttgcc agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgatctgct
    14101 gtgcgatccg caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga
    14161 ggttaaaact acagccgccg agtttggcat tgaactgacg gcgattggcg aactggtgcc
    14221 agcgcgcggc ggtcgtgcca tggttgagat ccgttaattc aatgcggttg tttattgccg
    14281 aaaaaccgag tctggcgcgc gccattgctg atgtcctgcc caaaccgcac cgtaaaggcg
    14341 atggctttat cgagtgcggt aatggtcagg tggtgacctg gtgtatcggt cacctgcttg
    14401 agcaggcgca gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc
    14461 cgattgtccc ggaaaagtgg caattacagc cacgaccctc cgtgaccaaa caacttaacg
    14521 tcatcaaacg cttcctgcat gaagccagcg aaatcgttca cgccggggac ccggatcgtg
    14581 aagggcaatt gctggtggat gaagtgctgg actatctgca actggcaccg gaaaagcgcc
    14641 agcaggtgca gcgttgcttg ataaacgacc tgaacccgca ggcggttgag cgggcgatcg
    14701 accgtcttcg ttccaacagt gagtttgtac cgctgtgcgt ttctgcgctg gcgcgagcgc
    14761 gtgccgactg gctgtacggc atcaatatga cccgtgctta caccattctc ggtcgcaatg
    14821 ccggttatca gggcgtgctt tccgtcggac gcgtgcagac gcccgtgctt gggctggtgg
    14881 tgcgccgcga tgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac
    14941 atatcgtgac acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg
    15001 aaccgtacca ggatgaagaa gggcgcttgt tacatcgtcc actggcggag catgtggtta
    15061 accgcatcag cggtcaaccg gctattgtca ccagctataa cgataaacgg gaatcagaat
    15121 ccgcgccgct gccgttttcg ctttcggcgt tgcagattga agcggcaaaa cgctttggtc
    15181 tgagcgcgca gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca
    15241 cttatccgcg ttccgattgt cgctatttgc cagaagaaca tttt
//
LOCUS       DAEVZA010000117        15055 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEVZA010000117 DAEVZA010000000
VERSION     DAEVZA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.117"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(317..511)
                     /locus_tag="LAF92_003771"
     CDS             complement(317..511)
                     /locus_tag="LAF92_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HBI9792705.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            900..1445
                     /locus_tag="LAF92_003772"
     CDS             900..1445
                     /locus_tag="LAF92_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBI9792706.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQSSEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1420..3345
                     /locus_tag="LAF92_003773"
     CDS             1420..3345
                     /locus_tag="LAF92_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBI9792707.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGKEQYLKFGDKETPFGLKWMPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLAEYARALSGEDE"
     gene            3342..3548
                     /locus_tag="LAF92_003774"
     CDS             3342..3548
                     /locus_tag="LAF92_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HBI9792708.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3545..5146
                     /locus_tag="LAF92_003775"
     CDS             3545..5146
                     /locus_tag="LAF92_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBI9792709.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5127..6446
                     /locus_tag="LAF92_003776"
     CDS             5127..6446
                     /locus_tag="LAF92_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBI9792710.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6456..6788
                     /locus_tag="LAF92_003777"
     CDS             6456..6788
                     /locus_tag="LAF92_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBI9792711.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6844..7868
                     /locus_tag="LAF92_003778"
                     /pseudo
     CDS             6844..7868
                     /locus_tag="LAF92_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            7910..8308
                     /locus_tag="LAF92_003779"
     CDS             7910..8308
                     /locus_tag="LAF92_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBI9792712.1"
                     /translation="MTKDELIARLRSLGEQMNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8320..8673
                     /locus_tag="LAF92_003780"
     CDS             8320..8673
                     /locus_tag="LAF92_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HBI9792713.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8685..9263
                     /locus_tag="LAF92_003781"
     CDS             8685..9263
                     /locus_tag="LAF92_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9792714.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPAAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9260..9655
                     /locus_tag="LAF92_003782"
     CDS             9260..9655
                     /locus_tag="LAF92_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9792715.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            9663..10403
                     /locus_tag="LAF92_003783"
     CDS             9663..10403
                     /locus_tag="LAF92_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9792716.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            10419..10841
                     /locus_tag="LAF92_003784"
     CDS             10419..10841
                     /locus_tag="LAF92_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBI9792717.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            10823..11257
                     /locus_tag="LAF92_003785"
     CDS             10823..11257
                     /locus_tag="LAF92_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBI9792718.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGSEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11250..13811
                     /locus_tag="LAF92_003786"
     CDS             11250..13811
                     /locus_tag="LAF92_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBI9792719.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSVSGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWAERTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GKFEQNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            13808..14137
                     /locus_tag="LAF92_003787"
     CDS             13808..14137
                     /locus_tag="LAF92_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9792720.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14137..14835
                     /locus_tag="LAF92_003788"
     CDS             14137..14835
                     /locus_tag="LAF92_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBI9792721.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPITWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14840..>15055
                     /locus_tag="LAF92_003789"
     CDS             14840..>15055
                     /locus_tag="LAF92_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HBI9792722.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     KYFRMSPEDWLQAEMQGEIVALVHSHPG"
BASE COUNT         3435 a         3785 c         4699 g         3136 t
ORIGIN      
        1 aacaattaaa cgatagttaa atggattgat gaaaattaaa actatatagg tggatgctca
       61 gcctattgga ggaggggggc actcagaatc ctgtggaatg aaataaaccg ctctttctgt
      121 ccattaccct tttagctgcg ctgtatcgtc gccgtattcc cgcattaacc atgaccgtag
      181 cccgacgggg aattccttct gcgtgagtgt gcgggaataa tcaaaaacga tgcacaccgg
      241 gttttactgt gctgacagac gcagggttac cctcatagtc gcttttccgg tgcgatggtg
      301 gaagaaaccg ggatgtttat tcatcatcac tttggattga tgtatatgct ctcttttctg
      361 acgttagtct ccgacggcag gcttcaatga cccaggctga gaaattccca gacccttttt
      421 gctcaagagc gatgttaatt tgttcaatca tttggttagg aaagcggatg ttgcgggttg
      481 ttgttctgcg ggttctgttc ttagttgaca tgaggttgcc ccgtattcag tgtcgctgat
      541 ttgtattgtc tgaagttgtt tttacgttaa gttgatgcag atcaattaat acgatacctg
      601 cgtcataatt gattatttga cgtggtttga tggcgtagat gcacgttgtg acatgtagat
      661 gataattatt atcattttgc gggtcctttc cggcgatccg acaggttacg gggcggcgac
      721 ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa ggcgtttccg ttcttcttcg
      781 tcgtaactta atgtttttat ttaaaatacc ccctgaaaag aaaggaaacg acaggtgctg
      841 aaaacgaact tttgggcctt tgtcgtttcc tttctctgtt tttggccgtg gaatgaacaa
      901 tggaagtcaa caaaaagcag ctggctgaca ttttcggtgc gagtatccgt accattcaga
      961 actggcagga acagggaatg cccgttctgc gaggcggtgg caagggtaat gaggtgcttt
     1021 atgactctgc cgccgttata aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa
     1081 agctgcgccg ggaggttgaa gaactgcggc agtccagcga ggcagatctc cagccaggga
     1141 ctattgagta cgaacgccat cgacttacgc gtgcgcaggc cgacgcacag gaactgaaga
     1201 atgccagaga ctccgctgaa gtggtggaaa ccgcattctg tactttcgtg ttgtcgcgga
     1261 tcgcaggtga aattgccagt attctcgacg ggatccccct gtcggtgcag cggcgttttc
     1321 cggaactgga aaaccgacat gttgatttcc tgaaacggga tatcatcaaa gccatgaaca
     1381 aagcagccgc gctggatgaa ctgataccgg ggttgctgag tgaatatatc gaacagtcag
     1441 gttaacaggc tgcggcattt tgtccgcgcc gggcttcgct cactgttcag gccggagcca
     1501 cagaccgccg ttgaatgggc ggatgctaat tactatctcc caaaagaatc cgcataccag
     1561 gaagggcgct gggaaacact gccctttcag cgggccatca tgaatgcgat gggcagtgac
     1621 tacatccgcg aggtgaatgt ggtgaagtct gcccgtgttg gttattccaa aatgctgctg
     1681 ggtgtttatg cctacttcat agagcataag cagcgcaaca cccttatctg gttgccgacg
     1741 gatggtgatg ccgagaactt tatgaaaact cacgttgagc cgaccatccg tgatattcct
     1801 tcgctgctgg cgctggcccc gtggtatggc aaaaagcacc gggataacac gctcaccatg
     1861 aagcgtttca ccaatggtcg tggcttctgg tgcctgggcg gtaaagcggc aaaaaactac
     1921 cgtgaaaagt cagtggatgt ggcgggttat gatgaacttg ctgcctttga tgaggatatt
     1981 gaacaggaag gctctccgac gttcctgggc gataagcgta ttgaaggctc ggtctggcca
     2041 aagtccatcc gtggctccac gcccaaagtg agaggcacct gccagattga gcgtgcagcc
     2101 agtgaatccc cgcattttat gcgttttcat gttgcctgcc cgcactgcgg gaaggagcag
     2161 taccttaaat ttggcgacaa agagacgccg tttggcctca aatggatgcc ggatgatccc
     2221 tccagcgtgt tttatctctg tgagcataat gcctgcgtca tccgtcagca ggagctggac
     2281 tttactgatg cccgttatat ctgcgaaaag accgggatct ggacccgtga tggcattctc
     2341 tggttttcgt catccggtga agagattgaa ccgcctgaca gtgtgacctt tcacatctgg
     2401 acggcgtaca gcccgttcac cacctgggtg cagattgtaa aagactggat gaagacgaaa
     2461 ggggatacgg gaaaacgtaa aaccttcgtg aacaccacgc tcggtgagac gtgggaagcg
     2521 aaaatcggcg aacgtccgga tgctgaagtg atggcagagc ggaaagagca ttattcagcg
     2581 cccgttcctg accgtgtggc ttacctgacc gccggtatcg actcccagct ggaccgctac
     2641 gaaatgcgcg tatggggatg ggggccgggt gaggaaagtt ggctgattga ccggcagatt
     2701 attatgggtc gccacgacga tgaacagacg ctgctgcgtg tggatgaggc catcaataaa
     2761 acctataccc gccggaatgg tgcagaaatg tcgatatccc gtatctgctg ggatactggc
     2821 gggattgacc cgaccattgt gtatgaacgc tcgaaaaagc atgggctgtt ccgggtgatc
     2881 cccattaaag gggcatccgt ctacggtaag ccggtggcca gcatgcctcg taagcgaaac
     2941 aaaaacgggg tttaccttac cgaaatcggt acggataccg cgaaagagca gatttataac
     3001 cgcttcacac tgacgccgga aggggatgaa ccgcttcccg gtgccgttca cttcccgaat
     3061 aacccggata tttttgatct gaccgaagcg cagcagctga cggctgaaga gcaggtcgaa
     3121 aaatgggtgg atggcaggaa aaaaatactg tgggacagta aaaagcgacg caatgaggca
     3181 ctcgactgct tcgtttatgc gctggcggcg ctgcgcatca gtatttcccg ctggcagctg
     3241 gatctcagcg cactgctggc gagcctgcag gaagaggatg gtgcagcaac caacaagaaa
     3301 acactggcag aatacgcccg tgccttatcc ggagaggatg aatgacgcga caggaagaac
     3361 ttgccgctgc ccgtgcggca ctgcatgacc tgatgacagg aaaacgggtg gcaacggtac
     3421 agaaagacgg acggcgagtg gagtttacga ccacttccgt gtctgacctg aaaaaataca
     3481 ttgctgagct ggaagtgcag accggcatga cacagcgacg caggggacct gcaggatttt
     3541 atgtatgaaa atgtccacca ttcccaccct tctggggccg gacggcatga catcgctgcg
     3601 tgaatatgcc ggttatcacg gcggtggcag cggatttggt gggcagttgc gggcgtggaa
     3661 cccaccgagt gaaagtgtgg atgcagccct gctgcccaac tttacccgtg gcaatgcccg
     3721 cgcagacgat ctggtacgca ataacggcta tgccgccaac gccatccagc tgcatcagga
     3781 tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat
     3841 cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc
     3901 cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg
     3961 ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac
     4021 cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa
     4081 cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg
     4141 tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc cgcagaaatg
     4201 gacatggata ccccgtgagt tacccggcgg gcgcgcctcg ttcattcacg tttttgaacc
     4261 cgtggaggac gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa
     4321 gatgctcgac acgctgcaga acacgcagct gcagagcgcc attgtgaagg cgatgtatgc
     4381 cgccaccatt gagagtgagc tggatacgca gtcagcgatg gattttattc tgggcgcgaa
     4441 cagtcaggag cagcgggaaa ggctgaccgg ctggattggt gaaattgccg cgtattacgc
     4501 cgcagcgccg gtccggctgg gaggcgcaaa agtaccgcac ctgatgccgg gtgactcact
     4561 gaacctgcag acggctcagg atacggataa cggctactcc gtgtttgagc agtcactgct
     4621 gcggtatatc gctgccgggc tgggtgtctc gtatgagcag ctttcccgga attacgccca
     4681 gatgagctac tccacggcac gggccagtgc gaacgagtcg tgggcgtact ttatggggcg
     4741 gcgaaaattc gtcgcatccc gtcaggcgag ccagatgttt ctgtgctggc tggaagaggc
     4801 catcgttcgc cgcgtggtga cgttaccttc aaaagcgcgc ttcagttttc aggaagcccg
     4861 cagtgcctgg gggaactgcg actggatagg ctccggtcgt atggccatcg atggtctgaa
     4921 agaagttcag gaagcggtga tgctgataga agccggactg agtacctacg agaaagagtg
     4981 cgcaaaacgc ggtgacgact atcaggaaat ttttgcccag caggtccgtg aaacgatgga
     5041 gcgccgtgca gccggtctta aaccgcccgc ctgggcggct gcagcatttg aatccgggct
     5101 gcgacaatca acagaggagg agaagagtga cagcagagct gcgtaatctc ccgcatattg
     5161 ccagcatggc ctttaatgag ccgctgatgc ttgaacccgc ctatgcgcgg gttttctttt
     5221 gtgcgcttgc aggccagctt gggatcagcc gcctgacgga tgcggtgtcc ggcgacagcc
     5281 tgactgccca ggaggcactc gcgacgctgg cattatccgg tgatgatgac ggaccacgac
     5341 aggcccgcag ttatcaggtc atgaacggca tcgccgtgct gccggtgtcc ggcacgctgg
     5401 tcagccggac gcgggcgctg cagccgtact cggggatgac cggttacaac ggcattatcg
     5461 cccgtctgca acaggctgcc agcgatccga tggtggacgg cattctgctc gatatggaca
     5521 cgcccggcgg aatggtggca ggggcatttg actgcgctga catcatcgcc cgtgtgcgtg
     5581 acataaaacc ggtatgggcg ctggccaatg acatgaactg cagtgcaggg cagctgcttg
     5641 ccagcgccgc ctcccggcgt ctggtcacgc agaccgcccg gacaggctcc atcggcgtca
     5701 tgatggctca cagtaattac ggtgctgcgc tggagaaaca gggcgtggaa atcacgctga
     5761 tttacagcgg cagccataag gtggatggca acccctacag ccatcttccg gatgacgtcc
     5821 gggagacact gcagtcccgg atggatgcaa cccgccggat gtttgcgcag aaggtgtcgg
     5881 catataccgg cctgtccgtg caggctgtgc tggataccga ggctgcagtg tacagcggtc
     5941 aggaggccat tgatgccgga ctggctgatg aacttgtcaa cagcaccgat gcgatcaccg
     6001 ttatgcgtga tgcactggat gcacgtaaat cccgtctctc aggagggcga atgaccaaag
     6061 agactcaatc aacaactgtt tcagccactg cttcgcaggc tgacgttact ggcgtggtgc
     6121 aagcgacgga gggcgaaaac gccagcgcgg cgcagccgga cgtgaacgcg cagatcaccg
     6181 ctgcggttgc ggcagaaaac agccgcatta tggggatcct caactgtgag gaggctcacg
     6241 gacgcgaaga acaggcacgc gtgctggcag aaacccccgg tatgaccgtg aaaacggccc
     6301 gccgcattct ggccgcagca ccacagagtg cacaggcgcg cagtgacact gcgctggatc
     6361 gtctgatgca gggggcaccg gcaccgctgg ctgcaggtaa cccggcatct gatgccgtta
     6421 acgatttgct gaacacacca gtgtaaggga tgtttatgac gagcaaagaa acctttaccc
     6481 attaccagcc gcagggcaac agtgacccgg ctcataccgc aaccgcgccc ggcggattga
     6541 gtgcgaaagc gcctgcaatg accccgctga tgctggacac ctccagccgt aagctggttg
     6601 cgtgggatgg caccaccgac ggtgctgccg ttggcattct tgcggttgct gctgaccaga
     6661 ccagcaccac gctgacgttc tacaagtccg gcacgttccg ttatgaggat gtgctctggc
     6721 cggaggctgc cagcgacgag acgaaaaaac ggaccgcgtt tgccggaacg gcaatcagca
     6781 tcgtttaact ttacccttca tcactaaagg ccgcctgtgc ggcttttttt acgggatttt
     6841 tttatgtcga tgtacacaac cgcccaactg ctggcggcaa atgagcagaa atttaagttt
     6901 gatccgctgt ttctgcgtct ctttttccgt gagagctatc ccttcaccac ggagaaagtc
     6961 tatctctcac aaattccggg actggtaaac atggcgctgt acgtttcgcc gattgtttcc
     7021 ggtgaggtta tccgtacccg tggcggctcc acctctgaat ttacgccggg atatgtcaag
     7081 ccgaagcatg aagtgaatcc gcagatgacc tgcgtcgcct gccggatgaa gatccgcaga
     7141 atctggcgga cccggcttac cgccgccgtc gcatcatcat gcagaacatg cgtgacgaag
     7201 agctggccat tgctcaggtc gaagagatgc aggcagtttc tgccgtgctt aagggcaaat
     7261 acaccatgac cggtgaagcc ttcgatccgg ttgaggtgga tatgggccgc agtgaggaga
     7321 ataacatcac gcagtccggc ggcacggagt ggagcaagcg tgacaagtcc acgtatgacc
     7381 cgaccgacga tatcgaagcc tacgcgctga acgccagcgg tgtggtgaat atcatcgtgt
     7441 tcgatccgaa aggctgggcg ctgttccgtt ccttcaaagc cgtcaaggag aagctggata
     7501 cccgtcgtgg ctctaattcc gagctggaga cagcggtgaa agacctgggt aaagcggtgt
     7561 cctataaggg gatgtatggc gatgtggcca tcgtcgtgta ttccggacag tacgtggaaa
     7621 acggcgtcaa aaagaacttc ctgccggaca acacgatggt gctggggaac actcaggcac
     7681 gcggtctgcg cacctatggc tgcattcagg atgcggacgc acagcgcgaa ggcattaacg
     7741 cctctgcccg ttacccgaaa aactgggtga ccaccggcga tccggcgcgt gagttcacca
     7801 tgattcagtc agcaccgctg atgctgctgg ctgaccctga tgagttcgtg tccgtacaac
     7861 tggcgtaatc atggcccttc ggggccattg tttctctgtg gaggagtcca tgacgaaaga
     7921 tgaactgatt gcccgtctcc gctcgctggg tgaacaaatg aaccgtgatg tcagcctgac
     7981 ggggacgaaa gaagaactgg cgctccgtgt ggcagagctg gaagaggagc ttgatgacac
     8041 ggatgaaact gccggtcagg acacccctct cagccgggaa aatgtgctga ccggacatga
     8101 aaatgaggtg ggatcagcgc agccggatac cgtgattctg gatacgtctg aactggtcac
     8161 ggtcgtggca ctggtgaagc tgcatactga tgcacttcac gccacgcggg atgaacctgt
     8221 ggcatttgtg ctgccgggaa cggcgtttcg tgtctctgcc ggtgtggcag ccgaaatgac
     8281 agagcgcggc ctggccagaa tgcaataacg ggaggcgctg tggctgattt cgataacctg
     8341 ttcgatgctg ccattgcccg cgccgatgaa acgattcgcg ggtacatggg aacgtcagcc
     8401 accatgacat ccggtgagca gtcaggtgcg gtgatacgtg gtgtttttga tgaccctgaa
     8461 aatatcagct atgccggaca gggcgtgcgc gttgaaggct ccagcccgtc cctgtttgtc
     8521 cggactgatg aggtgcggca gctgcggcgt ggagacacgc tgaccatcgg tgaggaaaat
     8581 ttctgggtag atcgggtttc gccggatgat ggtggaagct gtcatctctg gcttggacgg
     8641 ggcgtaccgc ctgccgttaa ccgtcgccgc tgaaaggggg atgtatggcc ataaaaggtc
     8701 ttgagcaggc cgttgaaaac ctcagccgta tcagcagaac ggcggtgccc gctgccgccg
     8761 caatggccat taaccgcgtt gcttcatccg cgatatcgca gtcggcgtca caggttgccc
     8821 gtgagacaaa ggtacgccgg aaactggtaa aggaaagggc caggctgaaa agggccacgg
     8881 tcaaaaatcc gcaggccaga atcaaagtta accgggggga tttgcccgta atcaagctgg
     8941 gtaatgcgcg ggttgtcctt tcgcgccgca ggcgtcgtaa aaaggggcag cgttcatccc
     9001 tgaaaggtgg cggcagcgtg cttgtggtgg gtaaccgtcg tattcccggc gcgtttattc
     9061 agcaactgaa aaatggccgg tggcatgtca tgcagcgtgt ggccgggaaa aaccgttacc
     9121 ccattgatgt ggtgaaaatc ccgatggcgg tgccgctgac cacggcgttt aaacaaaata
     9181 ttgagcggat acggcgtgaa cgtcttccga aagagctggg ctatgcgctg cagcatcaac
     9241 tgaggatggt aataaagcga tgaaacatac tgaactccgt gcagccgtac tggatgcact
     9301 ggagaagcat gacaccgggg cgacgttttt tgatggtcgc cccgctgttt ttgatgaggc
     9361 ggattttccg gcagttgccg tttatctcac cggcgctgaa tacacgggcg aagagctgga
     9421 cagcgatacc tggcaggcgg agctgcatat cgaagttttc ctgcctgctc aggtgccgga
     9481 ttcagagctg gatgcgtgga tggagtcccg gatttatccg gtgatgagcg atatcccggc
     9541 actgtcagat ttgatcacca gtatggtggc cagcggctat gactaccggc gcgacgatga
     9601 tgcgggcctg tggagttcag ccgatctgac ttatgtcatt acctatgaaa tgtgaggacg
     9661 ctatgcctgt accaaatcct acaatgccgg tgaaaggtgc cgggaccacc ctgtgggttt
     9721 ataaggggag cggtgaccct tatgcgaatc cgctttcaga cgttgactgg tcgcgtctgg
     9781 caaaagttaa agacctgacg cccggcgaac tgaccgctga gtcctatgac gacagctatc
     9841 tcgatgatga agatgcagac tggactgcga ccgggcaggg gcagaaatct gccggagata
     9901 ccagcttcac gctggcgtgg atgcccggag agcaggggca gcaggcgctg ctggcgtggt
     9961 ttaatgaagg cgatacccgt gcctataaaa tccgcttccc gaacggcacg gtcgatgtgt
    10021 tccgtggctg ggtcagcagt atcggtaagg cggtgacggc gaaggaagtg atcacccgca
    10081 cggtgaaagt caccaatgtg ggacgtccgt cgatggcaga agatcgcagc acggtaacag
    10141 cggcaaccgg catgaccgtg acgcctgcca gcacctcggt ggtgaaaggg cagagcacca
    10201 cgctgaccgt ggccttccag ccggagggcg taaccgacaa gagctttcgt gcggtgtctg
    10261 cggataaaac aaaagccacc gtgtcggtca gtggtatgac catcaccgtg aacggcgttg
    10321 ctgcaggcaa ggtcaacatt ccggttgtat ccggtaatgg tgagtttgct gcggttgcag
    10381 aaattaccgt caccgccagt taatccggag agtcagcgat gttcctgaaa accgaatcat
    10441 ttgaacataa cggtgtgacc gtcacgcttt ctgaactgtc agccctgcag cgcattgagc
    10501 atctcgccct gatgaaacgg caggcagaac aggcggagtc agacagcaac cggaagttta
    10561 ctgtggaaga cgccatcaga accggcgcgt ttctggtggc gatgtccctg tggcataacc
    10621 atccgcagaa gacgcagatg ccgtccatga atgaagccgt taaacagatt gagcaggaag
    10681 tgcttaccac ctggcccacg gaggcaattt ctcatgctga aaacgtggtg taccggctgt
    10741 ctggtatgta tgagtttgtg gtgaataatg cccctgaaca gacagaggac gccgggcccg
    10801 cagagcctgt ttctgcggga aagtgttcga cggtgagctg agttttgccc tgaaactggc
    10861 gcgcgagatg gggcgacccg actggcgtgc catgcttgcc gggatgtcat ccacggagta
    10921 tgccgactgg caccgctttt acagtaccca ttattttcat gatgttctgc tggatatgca
    10981 cttttccggg ctgacgtaca ccgtactcag cctgtttttc agcgatccgg atatgcatcc
    11041 gctggatttc agtctgctga accggcgcga ggctgacgaa gagcctgaag atgatgtgct
    11101 gatgcagaaa gcggcagggc ttgccggagg tgtccgcttt ggcccggacg ggagtgaagt
    11161 tatccccgct tccccggatg tggcggacat gacggaggat gacgtaatgc tgatgacagt
    11221 atcagaaggg atcgcaggag gagtccggta tggctgaacc ggtaggcgat ctggtcgttg
    11281 atttgagtct ggatgcggcc agatttgacg agcagatggc cagagtcagg cgtcattttt
    11341 ccggtacgga aagtgatgcg aaaaaaacag cggcagtcgt tgaacagtcg ctgagccgac
    11401 aggcgctggc tgcacagaaa gcggggattt ccgtcgggca gtataaagcc gccatgcgta
    11461 tgctgcctgc acagttcacc gacgtggcca cgcagcttgc aggcgggcaa agtccgtggc
    11521 tgatcctgct gcaacagggt ggtcaggtta aggactcctt cggcgggatg atccccatgt
    11581 tccgggggct tgccggtgcg atcaccctgc cgatggtggg ggccacctcg ctggcggtgg
    11641 cgaccggtgc gctggcgtat gcctggtatc agggcaactc aaccctgtcc gatttcaaca
    11701 aaacgctggt cctttccggc aatcaggcgg gactgacggc agatcgtatg ctggtcctgt
    11761 ccagagccgg gcaggcggca gggctgacgt ttaaccagac cagcgagtca ctcagcgcac
    11821 tggtcaaggc gggggtaagc ggtgaggctc agattgcgtc catcagccag agtgtggcgc
    11881 gtttctcctc tgtatccggc gtggaggtgg acaaggtcgc tgaagccttc gggaagctga
    11941 ccacagaccc gacgtcgggg ctgacggcga tggcacgcca gttccataac gtgacggcgg
    12001 agcagattgc gtatgttgct cagttgcagc gttccggcga tgaagccggg gcattgcagg
    12061 cggcgaacga ggccgcaacg aaggggtttg atgaccagac ccgccgcctg aaagagaaca
    12121 tgggcacgct ggagacctgg gcagagagga cagcgcgggc attcaaatcc atgtgggatg
    12181 cggtgctgga tattggtcgt cctgataccg cgcaggagat gctgattaag gcagaggctg
    12241 cgtttaagaa agcagacgac atctggaatc tgcgcaagga tgattatttt gttaacgatg
    12301 aagcgcgggc gcgttactgg gatgatcgtg aaaaggcccg tcttgcgctt gaagccgccc
    12361 gaaagaaggc tgagcagcag actcaacagg acaaaaatgc gcagcagcag agcgataccg
    12421 aagcgtcacg gctgaaatat accgaagagg cgcagaaggc ttacgaacgg ctgcagacgc
    12481 cgctggagaa atataccgcc cgtcaggaag aactgaacaa ggcactgaaa gacgggaaaa
    12541 tcctgcaggc ggattacaac acgctgatgg cggcggcgaa aaaggattat gaagcgacgc
    12601 tgaaaaagcc gaaacagtcc ggcgtgaagg tgtctgcggg cgatcgtcag gaagacagtg
    12661 ctcatgctgc cctgctgacg cttcaggcag aactccggac gctggagaag catgccggag
    12721 cgaatgagaa aatcagccag cagcgccggg atttgtggaa ggcggagagt cagttcgcgg
    12781 tactggagga ggcggcgcaa cgtcgccagc tgtctgcaca ggagaaatcc ctgctggcgc
    12841 ataaagatga gacgctggag tacaaacgcc agctggctgc acttggcgac aaggtcacct
    12901 atcaggagcg cctgaacgcg ctggcgcagc aggcggataa attcgcacag cagcaacggg
    12961 caaaacgggc cgccattgat gcgaaaagcc gggggctgac tgaccggcag gcagaacggg
    13021 aagccacgga acagcgcctg aaggaacagt atggcgataa tccgctggcg ctgaataacg
    13081 tcatgtcaga gcagaaaaag acctgggcgg ctgaagacca gcttcgcggg agctggatgg
    13141 caggcctgaa gtccggctgg agtgagtggg aagagagcgc cacggacagt atgtcgcagg
    13201 ttaaaagtgc agccacgcag acctttgatg gtattgcaca gaatatggcg gcgatgctga
    13261 ccggcagtga gcagaactgg cgcagcttca cccgttccgt actgtccatg atgacagaaa
    13321 ttctgcttaa gcaggcaatg gtggggattg tcgggagtat cggcagcgcc attggcgggg
    13381 ctgttggtgg cggcgcatca gcgtcaggcg gtacagccat tcaggcagct gcggcgaaat
    13441 tccattttgc gaccggagga tttacgggaa ccggcggcaa atatgagcca gcggggattg
    13501 ttcaccgtgg tgaatttgtc ttcactaagg aggcaaccag ccggattggc gtggggaatc
    13561 tctaccggct gatgcgcggc tatgccaccg gcggttatgt cggtacaccg ggcagcatgg
    13621 cggacagccg ctcgcaggcg tccgggaagt ttgagcagaa taaccatgtg gtgattaaca
    13681 acgacggcac gaacggtcag atagggccac aggcgctgaa ggcggtttat gacgtagccc
    13741 gtaaggcggc aatggatgtt gtgaccgggc agatgcgcga tggtggtctg ttctccggag
    13801 gtggacgatg aaaaccttcc gctggaaagt gaaacccggt atggatgtgg cttcggcccc
    13861 ttccgtaaga aaggtgcgct ttggtgatgg ctattctcag cgagcgcctg ccgggctgaa
    13921 tgccgacctg aaaacgtaca gcgtgacgct ttctgttccc cgttgggagg ccacggcgct
    13981 tgagtcgttt ctggctgagc acgggggctg gaaatccttt ctgtggacgc cgccttatga
    14041 gtggcggcag ataaaggtga cctgcgctaa atggtcgtcg cgggtcagta tgctgcgtgt
    14101 tgagttcagc gcagagtttg aacaggtggt gaactgatgc aggatatccg gcaggaaaca
    14161 ctgaatgaat gcacccgtgc ggagcagtcg gccagcgtgg tgctctggga aatcgatctg
    14221 acagaggtcg gtggagaacg ttattttttc tgtaatgagc agaacgaaaa aggtgagccg
    14281 atcacctggc aggggcgaca gtatcagccg tatcccattc aggggagtgg ttttgaactg
    14341 aatggcaaag gcaccagtac gcgccccacg ctgacggttt ctaacctgta cggtatggtc
    14401 accgggatgg cggaagatct gcagagtctg gtcggcggaa cggtggtccg gcgtaaggtt
    14461 tacgcccgtt ttctggatgc ggtgaacttc gtcaacggaa acagtgacgc cgatccggag
    14521 caggaggtga tcagccgctg gcgcattgag cagtgcagcg aactgagcgc ggtgagtgcc
    14581 tcttttgtac tgtccacgcc gacggaaacg gacggcgctg tttttccggg acgtatcatg
    14641 ctggccaaca cctgcacctg gacctatcgc ggtgatgagt gcggttatca cggtccggct
    14701 gtcgctgatg aatatgacca gccgacgtcc gatatcacga aggataaatg cagcaaatgc
    14761 ctgagtggtt gtaagtttcg caataacgtc ggcaactttg gcggcttcct ttccattaac
    14821 aaactttcgc agtaatccca tgacacagac agaatcagcg attctggcgc acgcccgacg
    14881 atgtgcgcca gcggagtcgt gcggcttcgt ggtgagaacg ccggaggggg aaagatattt
    14941 tccctgcgtg aatatctccg gtgagccgga gaagtatttc cggatgtcgc cggaggactg
    15001 gctgcaggca gaaatgcagg gtgagattgt ggcgctggtc cacagccacc ccggt
//
LOCUS       DAEVZA010000118        14992 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEVZA010000118 DAEVZA010000000
VERSION     DAEVZA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.118"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            228..470
                     /locus_tag="LAF92_003790"
     CDS             228..470
                     /locus_tag="LAF92_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HBI9792723.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGV"
     gene            474..761
                     /locus_tag="LAF92_003791"
     CDS             474..761
                     /locus_tag="LAF92_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792724.1"
                     /translation="MTEQEMPRYQCHKKVRAMKIGSIEHKPNPDQSGKTGSSSYGAII
                     HPDDKKYAAFDVSAEYICKHRPMSGGYYVVYEDGYESYSPAEVFESGYSKL"
     gene            771..950
                     /locus_tag="LAF92_003792"
     CDS             771..950
                     /locus_tag="LAF92_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792725.1"
                     /translation="MTSVVDLGKEKKFPITQELYERLESVIHDYDGEISLCEAIGTLE
                     LLKQSLIEGAKESSA"
     gene            974..1396
                     /locus_tag="LAF92_003793"
     CDS             974..1396
                     /locus_tag="LAF92_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HBI9792726.1"
                     /translation="MATEPKAGRPSDYMPEVADDICSLLSSGESLLKVCKRPGMPDKS
                     TVFRWLAKHEDFRDKYAKATEARADSIFEEIFEIADNAIPDAAEVAKARLRVDTRKWA
                     LARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            1393..2805
                     /locus_tag="LAF92_003794"
     CDS             1393..2805
                     /locus_tag="LAF92_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBI9792727.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADEVVHGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            2808..4934
                     /locus_tag="LAF92_003795"
     CDS             2808..4934
                     /locus_tag="LAF92_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBI9792728.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDPMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYAAEYGKKPPASLDVTSMTSWEYD
                     WFDADVIYIAKYYEVRKESVDVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            4948..5832
                     /locus_tag="LAF92_003796"
     CDS             4948..5832
                     /locus_tag="LAF92_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HBI9792729.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEV
                     ATEPEKTEETPEDYALRIGDEEIQLNADDDDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKPAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            5844..7115
                     /locus_tag="LAF92_003797"
     CDS             5844..7115
                     /locus_tag="LAF92_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head protein"
                     /protein_id="HBI9792730.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISEQNKNNLNSGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATTSVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVA
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            7158..7343
                     /locus_tag="LAF92_003798"
     CDS             7158..7343
                     /locus_tag="LAF92_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792731.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFEKLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            7318..7800
                     /locus_tag="LAF92_003799"
     CDS             7318..7800
                     /locus_tag="LAF92_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein p27"
                     /protein_id="HBI9792732.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDTPNA
                     "
     gene            7809..9227
                     /locus_tag="LAF92_003800"
     CDS             7809..9227
                     /locus_tag="LAF92_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HBI9792733.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            9227..9928
                     /locus_tag="LAF92_003801"
     CDS             9227..9928
                     /locus_tag="LAF92_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9792734.1"
                     /translation="MADSNLNVPVIIQATRLDTSILPRNIFSQSYLLYVINQGADVGA
                     IAGKANQAGQGAYDAQVKNDEQDVELADHDARITANTKAINLLEVRLTTAEGKIVVLR
                     SDVDYLLDEVIAIQAHLVTVDQRLDGIESDVSDIKSDYVSKTVTESQSLASPLDVKTS
                     YSVDGIQVVGARQTGWTAATGTPLLGSFNANQSYTVGTTYTQSEVAALATGLQQARQR
                     ILALETALRLHGLID"
     gene            9928..10383
                     /locus_tag="LAF92_003802"
     CDS             9928..10383
                     /locus_tag="LAF92_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HBI9792735.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            10386..11078
                     /locus_tag="LAF92_003803"
     CDS             10386..11078
                     /locus_tag="LAF92_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBI9792736.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLETSTPWGAG
                     IGAGIGLLGSLF"
     gene            11088..12494
                     /locus_tag="LAF92_003804"
     CDS             11088..12494
                     /locus_tag="LAF92_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HBI9792737.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSLNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            12494..14497
                     /locus_tag="LAF92_003805"
     CDS             12494..14497
                     /locus_tag="LAF92_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="injection protein"
                     /protein_id="HBI9792738.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQSAPTQQGVQQSP
                     ADNSLASGYAQLATQQKEGLDRSAEQGAVLGAAMRDAVTGESRMTPEMERLQNVGSAP
                     ELNSLSTDALRAGLGQLFGSDASQEKILQSIGGKIRKDEKGNSIVTLPSGEYALNKPG
                     LSPQDITSFLANALAFTPAGRAASVVGATLKSGATDLALQGATKIAGGENVNPVQTAI
                     SAGLGGVLKGVENTASAVSRSAMGKIAPEKQAQIDFAKQNNLPLMTTDLVEPGTNIGK
                     QARAMAERIPIAGTGGIRNAQQKAREDLVRTFSDNVGGISDAQLYQSATRGQQQFIQA
                     AGKRYDRIISLMGDTPVDITGTVKAIDDQISKLTRPGVSQDRSAVSVLQQFRNDITSG
                     PNNLQLARENRTNLRKRFMAAPDEVDRDTLEKAAQSVYNAYTTDMKKAVGAKLGAKEA
                     QNMSRVDRSWAKFNDMMSNTRVQKAIQSGKTTPEDVTKLVFSQSPAERAQLYRLLDDS
                     GRQNARAALVQRAMDKATSDSGKLSVEKFINEMKRNRKQAETFFRGEHGKQLDGIMKY
                     LDSTRQAATAAASPLTGQMVAGPAALITALASVTNPMFAKVAAVGAGIGMAGRGYESR
                     AMRNALLKLANTPKGSTAYDRAIRRVSETLTPLIQASSEKAQQ"
     gene            complement(14506..14826)
                     /locus_tag="LAF92_003806"
     CDS             complement(14506..14826)
                     /locus_tag="LAF92_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792739.1"
                     /translation="MYISLSTIFFICLAIWLLRIWQDCSVSHAAAVRNKNALIKEAEN
                     VVLSMDHLSWTEMTTGQQEVYECAIERLRLLKSYKKNHAPDSFPFLKEWPRWYDPKKA
                     TINR"
BASE COUNT         4251 a         3414 c         3853 g         3474 t
ORIGIN      
        1 ttattgaagc aaatgttgag tactggcgcg agcaactgca gttgactctc gatatcgcgt
       61 gctgatggca aacgcaaaac tgcgttatcg aaaaaatcaa agcattacta gaactgagca
      121 acggctatcc attacaaagc ccatctacgg gtgggcttga taatggctta taccctgcac
      181 gggataactt aactgatatc ccttttaacg gataaacgga gccaacaatg gcagagatta
      241 ttcccatgac tgaagaacag aaattccagt tagagattta caaactggtc atgaaccaga
      301 acgcagccgc agaagaagca tttcagttca ttggcactga cgagctgaag cttgagctat
      361 tcaaaattca cttccagtca ggcggcgcta attcagatat cacgacgcgc actatcgaag
      421 cggtgcgtaa atcgaaggaa gcgttagacc tgttcactac cggagtgtaa gagatgactg
      481 aacaagaaat gccgagatac cagtgccaca aaaaagttcg cgccatgaag attggctcta
      541 tagaacataa gccaaaccca gatcagtctg gtaagactgg ctcttctagt tatggggcaa
      601 ttattcatcc ggatgataag aaatacgcag catttgatgt tagcgcggaa tatatctgta
      661 agcaccgacc aatgtctgga ggctattacg ttgtctatga ggatggatat gaatcatatt
      721 ctcctgctga ggtatttgag tctggatatt caaaattata ggaatcctct atgacaagcg
      781 tcgttgatct tggtaaggag aagaaattcc caattactca agagctatac gagaggctgg
      841 aaagcgtcat ccatgattac gatggtgaaa tcagtttatg cgaggcgatt ggcacactcg
      901 aattgctgaa gcagtcactg attgaaggcg cgaaagagtc ctcagcctga aatgacaatt
      961 aagtgagata aatatggcga ctgaaccaaa agctggtcgc ccctctgatt atatgccgga
     1021 ggtggctgac gatatctgct cgttgctttc ttctggcgaa agtttgctga aagtatgtaa
     1081 gcgtcctggt atgccggata agtccactgt tttccgctgg ttggcaaagc atgaggattt
     1141 tcgcgacaag tacgcgaagg caactgaggc acgagctgat tctattttcg aagagatatt
     1201 cgaaattgct gacaatgcga ttccagatgc tgctgaagtg gcaaaggcaa gacttcgcgt
     1261 tgatacccgc aaatgggcgc tggcccgaat gaatccccgt aagtatggcg acaaggtaac
     1321 taacgagctt gtcggtaagg acggcggcgc aattcagatt gaaacatcac cgatgagcac
     1381 tctgttcgga aaatgacctc gattaatcct atctttgaac cgttcattga ggcgcatcgc
     1441 tacaaagtcg ccaaaggcgg tcgaggtagc ggtaagtcat gggcaattgc gaggctgctt
     1501 gttgaagcgg cgcgtcggca gcctgtgcgc atactttgcg ctcgtgagct gcaaaacagt
     1561 atcagcgatt cggtaattcg gttgcttgaa gacaccatag agcgggaagg gtattcggct
     1621 gagtttgaaa ttcagcgttc aatgattcgt catctcggaa cgaatgctga gttcatgttc
     1681 tacggcatca aaaacaaccc gacgaagatt aaatcgctcg aaggcattga tatctgctgg
     1741 gtggaggaag cggaagcggt aacgaaggaa tcatgggata tcctgatacc aaccatccgc
     1801 aagccatttt ccgaaatatg ggtgagcttc aacccgaaaa acatcctcga cgatacctat
     1861 cagcgattcg ttgtaaatcc tcccgatgat atttgcctgc tgacagtgaa ctacaccgac
     1921 aacccgcact ttcctgaagt tctccgtctg gagatggaag agtgcaaacg cagaaatccg
     1981 acactatatc gtcacatctg gcttggtgag ccagtgagcg caagtgatat ggcaatcatc
     2041 aaacgtgaat ggcttgaagc cgcaaccgat gcgcacaaga aactcggatg gaaggcgaaa
     2101 ggcgctgttg tctctgcgca tgacccgtca gatacaggac cggatgctaa aggttatgca
     2161 tcgcgccacg gttcggtagt taagcgcatt gccgaaggcc tgctgatgga catcaatgaa
     2221 ggtgctgact gggcaacttc gctggcgatt gaagacggcg ctgaccatta cctgtgggat
     2281 ggtgatggcg tcggtgcggg gctacgcaga cagacaacgg aagcgttctc cggcaagaaa
     2341 atcaccgcca cgatgttcaa gggcagcgaa tcgccattcg atgaagatgc accatatcag
     2401 gccggagcat gggccgatga agtcgtacat ggcgacaacg ttcgcactat tggcgatgta
     2461 ttccgcaata agcgagcgca attctattac gcgctggctg acaggctgta tctgacatat
     2521 cgggcggttg ttcacggtga gtatgcagac cccgacgaca tgctgagttt cgacaaagaa
     2581 gcgataggcg agaagatgct ggagaagctg tttgcagaac tgacgcagat tcagcgcaaa
     2641 ttcaataaca acgggaagct ggagctaatg actaaggtcg aaatgaagca gaagctcggt
     2701 attccatctc ctaacctggc tgatgcgctg atgatgtgta tgcattgccc ggcattggtc
     2761 cgcgaagaaa ctgaaatata cgttccctca tcctccggtt ggtaaacatg gcagagacat
     2821 tagagaaaaa acatgagcgg atcatgctca ggtttgaccg cgcctattct ccacagcagg
     2881 aagtgcgcga aaagtgcatt gaagctacga ggtttgctcg tgtccccgga ggtcaatggg
     2941 aaggagcaac ggcggctgga actaagcttg atgagcagtt cgagaagtat cctaagtttg
     3001 aaatcaacaa ggtagcaact gaacttaacc gcatcattgc agaataccgc aataatagaa
     3061 tcaccgttaa gtttcgtcct ggtgacagag aggcaagcga agagttagcc aataaattaa
     3121 atggtctgtt ccgtgctgac tacgaagaaa ctgatggcgg tgaggcttgc gataatgcat
     3181 ttgacgacgc tgctactggt ggtttcggtt gcttccgttt gacgtcgatg ctggtcaatg
     3241 aatacgaccc catggacgat cgtcagcgta ttgctattga accaatatac gacccgtcgc
     3301 gctctgtgtg gtttgaccct gacgctaaga agtacgacaa atctgacgcg ttgtgggcgt
     3361 tctgcatgta ttcgttgtca cctgaaaaat atgcggctga atacggaaag aaacctcctg
     3421 cttctctgga tgtaacgtct atgaccagtt gggaatatga ctggtttgat gcagatgtta
     3481 tttacatagc gaagtattac gaagttcgta aagagtctgt tgacgttatc agttatcgcc
     3541 atccaatcac tggagagatt gcaacatacg acagtgatca ggtcgaagat attgaagatg
     3601 aactggcaat agctggattt caggaagtgg caaggcgctc agtgaagcgc cgtcgtgtgt
     3661 atgtatccgt agtggatggt gatggtttcc ttgagaaacc tcgacgtatt cctggtgagc
     3721 atatccccct catcccggtt tatggaaaac gctggttcat tgatgacatt gagcgtgtcg
     3781 aaggacatat tgcaaaagca atggatccac agcgtttgta caaccttcag gtatcaatgc
     3841 tggctgatac tgcagcgcaa gacccaggtc agatccctat agttggcatg gagcaaatac
     3901 gtggacttga gaagcactgg gaggctcgca acaagaaacg accagcgttc ttgccgttgc
     3961 gcgaagtgag agataaatct ggcaacatca tcgctggagc taccccggca ggatatacac
     4021 agcctgcggt tatgaatcag gcattggctg cattactaca gcaaaccagt gcagatattc
     4081 aggaggttac aggcggcagt caggccatgc agcagatgcc aagtaatatt gctcaggaaa
     4141 cggttaacaa cttgatgaac agagcagata tggcttcgtt tatctatctg gacaatatgg
     4201 cgaaaagtct taaacgcgct ggtgaagtat ggctgtcaat ggcgcgtgaa gtgtacggtt
     4261 cagaacgtga agtgcgcatc gttaacgaag atggaagtga tgatatcgct gtcctgagcg
     4321 cacaggttgt tgacaggcaa acaggggctg ttgttgcgtt aaatgacctt tctgtcggtc
     4381 gatacgatgt gacggttgat gttggaccaa gctacacagc acgacgtgat gcaacggttt
     4441 ctgtactgac aaatgtcctt agctctatgc ttccaacaga cccaatgcgc ccggcaattc
     4501 agggtattat tctggacaat atcgatggcg aaggccttga tgacttcaaa gagtacaacc
     4561 gaaaccaact gctgatatct ggtattgcaa aaccacgcaa tgagaaagag cagcagattg
     4621 ttcaacaggc gcaaatggca gcacaaagcc agccaaatcc tgaaatggtt ctcgctcagg
     4681 cgcaaatggt agcagcgcag gcagaagcgc aaaaagcaac taacgaaact gctcaaactc
     4741 aaatcaaagc atttactgcc cagcaggatg cgatggagag tcaggcaaac actgtctata
     4801 aactggctca agccagaaac atcgatgaca aagcagtgat ggaggcaata cgccttctga
     4861 aagatgtcgc cgagtcacaa caacagcaat tccagtcacc accacagtca ccggcagact
     4921 taatgccgag ttaaccagga gtaatcaatg gaaaacgaac tgatcatcga cggtcaggtt
     4981 attgacctgt ctgaaacaca ggaaaatgca gaagaaacca tcatccaaac agagtcacag
     5041 cctgagaatg aaagccagga tgacaacggt aaagaggtgg caactgagcc tgaaaaaacc
     5101 gaagagacac cagaagatta cgccttgcgt attggtgatg aagaaattca gcttaacgct
     5161 gacgatgatg atcacattga cgggcaacct gcaccgcaat gggtgaaaga tcttcgcaaa
     5221 ggcttcaaag aaacacagaa agaaaaccgt gagttgcgcc gccagcttga ggaagcatta
     5281 gccaagccag cggaacatca gcaaccacaa ccagacgcta ttccaccaaa accgactctt
     5341 gagtcgtgtg attatgacga acaggcgttt gaacaggcat tgactgattg gcatgagaaa
     5401 aaaggccgtg tcgaacagca gcagcaacaa aaactacgtc agcaacagga ataccaacag
     5461 cgtttccagc aaagggtaga agcgcataaa caacgggcag ccaaacttcc tgtgaaagat
     5521 tatcaggaaa tggaggccat tgttcttagt gagctaccac caattcagca ggaaatcatc
     5581 attcactgtg cagacgaagg ctctgaacta ctcgcctatg gcttaggtaa gagccagcaa
     5641 ttacgccagc gtgtagccgc tgagacagat ccaattcgcg cagcattcct cttggggcag
     5701 attagcaaac aggtaagcct tgctccaaaa ccaaagaaag ccatcaagcc agagccggaa
     5761 gtacgtggtg gcggtgctga tgcgaaacaa gacgaattca acaaattatg tcccggcgca
     5821 aaaatcgaat aaggaaaaga taaatgccta acaatctcga cagtaacgtc agtcaaatcg
     5881 ttctgaaaaa attccttccg ggttttatgt cagatttagt tctggcgaaa accgtagacc
     5941 gtcagttgct ggcaggtgaa atcaactcca gcactggcga tagcgttagc tttaaacgtc
     6001 cgcatcaatt ctcatccctc cgtactccca ctggtgatat ttcagagcaa aataaaaaca
     6061 acctgaactc aggtaaagct acggggcgtg taggtaacta catcactgtt gctgttgaat
     6121 atcagcaact ggaggaagcg atcaagctta accaactgga agaaattctc gcgccggttc
     6181 gccagcgaat cgttaccgac cttgaaacag agcttgctca cttcatgatg aataacggtg
     6241 cgttgtcact tggtagcccc aatactccaa tcaccaaatg gtctgatgtt gcgcagacgg
     6301 catctttcct gaaagacctc ggcgttaatg aaggtgaaaa ctatgctgta atggatccat
     6361 ggtctgcaca gcgacttgct gatgcgcaga ctggtttgca tgcttcagat caattggttc
     6421 gtactgcatg ggagaacgca cagatcccaa ccaattttgg cggcattcgc gcactgatgt
     6481 ctaatgggct tgcctctcgt acgcaggggg catttggcgg aacactgaca gtcaaaacac
     6541 agccaactgt tacctataac gcagttaaag actcatacca gttcactgta acattgaccg
     6601 gagcgacaac cagcgttaca ggtttcctga aagctggtga tcaggttaaa ttcaccaata
     6661 cctactggct gcaacagcag accaaacagg cgttgtataa cggagccaca ccaattagct
     6721 tcactgcaac ggttactgct gatgctaatt cagacagcag tggcgatgtg acggttacgc
     6781 tttctggtgt tccgatttat gacactacaa acccgcagta caactctgta agtcgtcagg
     6841 tagcggcagg tgatgccgta tctgtagtag gcactgctag ccagacaatg aagccaaacc
     6901 tgttctataa caagttcttc tgtggacttg gctctatccc actgccgaaa ctgcacagta
     6961 ttgattctgc tgttgcaaca tatgaaggtt tctccatccg cgtacataaa tacgcagatg
     7021 gcgatgccaa cgtgcaaaaa atgcgctttg acttactgcc tgcatatgtg tgctttaacc
     7081 ctcacatggg cggtcagttc ttcggtaatc cgtaataaca aggggcttac gcccctttta
     7141 tgttttaagg aaacaatatg gatcgcatga gtgtattcct tgccgcagat aacgaatccg
     7201 ggcatgtaca ggccgttatc gcagaaaaag acttccagtt tttcgaaaag ttgggctttg
     7261 ttgcctcagt tgatgaattg aaaccgacca gtaagcgagg tcgtaaggcg gcagacaatg
     7321 gcaacagtac tgacaaaggg tgagatcgtc ctttttgcgc ttcgtaagtt tgctattgct
     7381 tctaatgcat cgctgactga tgttgagccg caatcaattg aagatggtgt aaatgatctg
     7441 gaagatatga tgtccgagtg gatgattaac cccggcgaca ttggttacgc tttcgcaact
     7501 ggagatgagc agccattacc agatgatgag tcaggtcttc caagaaaata caaacacgca
     7561 gtaggctatc agttattgct gagaatgcta tctgattaca gccttgaacc aactccgcaa
     7621 gttctcagta acgcccaacg ctcatatgat gccttgatga ccgacactct ggttgttcct
     7681 tcaatgcgac gacgtggaga ttttcctgta gggcagggta ataaatatga cgtgttcaca
     7741 tctgaccgat attatccagg cgatctccct ctgattgatg gcgatacccc aaacgcatag
     7801 gtgaataaat gcctattcag caacttccgc ttatgaaagg tgtcggcaaa gactttcgaa
     7861 acgccgacta tatcgactat ctgccagtga atatgttggc aacccccaaa gaaatcctga
     7921 acagcagcgg atatcttcgc tcattcccgg gcattgccaa acgctctgat gtaaacggcg
     7981 tatcgcgcgg cgtcgagtac aacatggcgc agaatgctgt ttatcgtgtg tgtggcggca
     8041 agctctacaa aggcgaaagc gaagtcggtg acgtcgccgg aagtggtcgc gtatcaatgg
     8101 cgcatgggcg gacatcacag gcggtaggcg ttaatggtca actggtcgaa taccgctatg
     8161 atggcacggt taaaaccgtc tcaaactggc ctacggacag cggattcaca caatacgagt
     8221 taggctcagt tcgcgacatt acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa
     8281 ctgattcatg gtttatcact gaccttgaag acgaatcgca ccctgaccga tacagcgcac
     8341 aatatcgtgc cgagtctcag ccggacggca tcatcggtat cggaacatgg cgagacttca
     8401 tcgtctgctt tggttcatcg acgattgaat atttctccct gactggtgca accaccgttg
     8461 gcgccgcttt gtatgtcgca cagccatcac tgatggtgca gaaaggcatt gccggaactt
     8521 actgcaaaac gccgtttgct gattcctatg cgtttatcag caatccggca acaggtgcgc
     8581 cgtctgtgta tatcatcggc tccggtcagg tatcaccaat cgccagcgcg agcattgaga
     8641 aaatactacg ctcctacact gctgatgaac tggctgatgg cgtgatggaa tcgttgcgct
     8701 ttgatgctca tgagttgctg attatccacc tgcctcgcca cgtcctagtg tatgacgcat
     8761 cttcaagcgc taatggtccg caatggtgtg tgctgaaaac aggcctgtat gacgatgtgt
     8821 accgcgctat cgacttcatt tacgaaggca atcagataac gtgcggcgat aagctagagt
     8881 ccgtgaccgg gaaattgcag ttcgatatca gcagccagta cgacaagcag caggaacacc
     8941 tgctgtttac tccgttgttc aaagcggata acgccagatg cttcgatctg gaggtggaat
     9001 catccactgg cgtagctcag tacgccgacc gtctgttcct ctctgcaacc actgacggca
     9061 tcaattacgg tcgtgagcag atgattgagc agaatgaacc gttcgtttac gacaaacgcg
     9121 ttttgtggaa gcgtgtcgga cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca
     9181 cgaagtcacc tgtaactctg tctggctgcc agataaggat tgagtaatgg ctgattcgaa
     9241 tctcaatgtg ccggtaatca ttcaggctac gcggctcgat acatcaatcc ttccacgcaa
     9301 tatattcagc cagtcttacc tgctgtatgt cataaatcag ggtgctgatg ttggcgcaat
     9361 tgccgggaag gcaaatcagg ctggtcaggg cgcttacgat gctcaggtaa aaaacgatga
     9421 acaggacgtc gaactggcag atcatgacgc aagaatcacc gcaaacacaa aagcgataaa
     9481 tctccttgag gtcaggttaa caaccgccga agggaagata gtcgtactgc gtagcgatgt
     9541 tgattacttg ctggatgagg ttatcgctat tcaggcgcat ctggtcactg ttgaccaaag
     9601 actggatggc atagaaagcg atgtatctga cattaagagt gattacgtat cgaaaaccgt
     9661 aaccgaatcg cagtctcttg cgtcaccgct ggatgtaaaa acatcatatt cagttgatgg
     9721 aattcaggta gttggagcaa ggcagactgg atggactgca gccacaggta cgccacttct
     9781 tggctcattc aacgcaaacc agtcatacac tgtcggcact acgtacacgc aatccgaagt
     9841 cgcagctctc gcaacaggtt tgcagcaggc gcggcagcgt attctggcgc ttgaaacagc
     9901 acttagatta catgggctga ttgactgatg attacattca aaccaacgcg aaacatcgac
     9961 ctgatcgaag cagtcggaaa tcaccctgac attatcgctg gtagcaacaa cggtgatgga
    10021 tacgactaca aacctgaatg ccgttacttt gaggtgaacg tgcacgggca gttcggcggc
    10081 attgtttact atcaggagat tcagccgctg acattcgatt gccacgccat gtatctgcca
    10141 gagattcgcg gcttcagcaa ggaaatcggg ctggcgttct ggcgatacat tctgactaac
    10201 accaccgttc agtgcgtaac atcgttcgct gcacgcaaat tccgccacgg gcagatgtac
    10261 tgcgcaatga ttggccttaa gcgtgtagga accatcaaga aatacttcaa aggcgtggat
    10321 gacgtgactt tttacagcgc cacacgcgaa gaactaatcg acttcctgaa tcacgggaga
    10381 tagctatgtt atatgcattt aagctgggca gaaaactgcg cggcgaggaa ccttattgcc
    10441 ctgaaaaagg cgggaaaggt ggcagctctg ataaaagcgc aaagtatgca gcagaagctc
    10501 agaagtatgc cgcagacctg caaaatcagc agtggcagac gatcatgaaa aaccttgctc
    10561 cgttcacgcc gcttgcggag cagtatgtta accagcttca gaatctttcc agtttagaag
    10621 gtcaggggca ggcacttaat cagtattaca actctcagca gtataaagac cttgcaggtc
    10681 aggcgcgtta ccagagtctt gctgctgcgg aggcgactgg tggacttggt tcgacagcca
    10741 caagcaatca actggctacg atcgcgccga ctctcggtca gtcttggtta tcaaaccaga
    10801 tgagcaatta caacaatctg gcaaacgttg ggcttggtgc tctgcaaggt caggcaaacg
    10861 ccgggcagac gtacgccaac aacatgagca gcattgcaca gcaaagcgca gctcttgccg
    10921 ctgctaatgc caacaaacca tcaagtcttc agacagcaat tagtggcgga acgtctggtg
    10981 cgattgccgg tgcaggtctt gccagccttt tggaaacatc aacaccttgg ggcgctggca
    11041 ttggtgctgg tatcggattg cttggttcgt tgttttaagg ggtaatcatg gctacttggc
    11101 aaggatcaaa tggcggattg ttggctggta tcggcggcgt caactcaaac gctccgagcg
    11161 taaatgacat cggcaatacg cttcagctta tcaggcagaa caatgatatt gagcgttcag
    11221 gcgctaacaa tgttgggctg actgctttgc aaggcctttc aggtattgcg ggggtgtttc
    11281 agcaggaaaa gcaggctcag cggcagaaag aatttcagca ggcatacgct aatgcttatg
    11341 cgtctggtga tcgcggtgct ttgcgtcagt tggctactca atatccagac cagattgaat
    11401 ccgttcgtaa aggcatggga ttcattgatg aagagcagcg taattctatc ggcaccttag
    11461 cagctggcgc acgccttgcg gcctcgtctc cagaagcaat gcaatcatgg ctgcaaaaca
    11521 acgccaagga actggcgcgc gtcggcgttg accctaacag cgttgctcag atgtatcagc
    11581 agaatccttc aggatttggt gagtttgttg atcaccttgg gatggctgct cttggtccaa
    11641 ttgattactt caatgttcag gacaagatgg ctggtcgtga gattgaccga ggcagactgg
    11701 cagagacaat ccgcagcaat caggctggtg aagcacttca ggcgagaggg caaaaccttt
    11761 cctatcagtc agcaatgact gggcacaata tcgcagcaca acgcttggct ctggatcagc
    11821 aagagttcgg gtttaagatg cagcaagcgc aggaaaaggc tcagcagttg attagtgaag
    11881 cacctaagct gtcagtaaac atggaaaaag gcatcgagac ggctgtaaac aatgccacag
    11941 catcatcaaa ctcagccaat tctatgagtg cgcttgctca acagttcaga gcagaaaaac
    12001 caacgacagg tttgttcggt aacgcacaga acatgttcgc aaaacttacc ggaagcgata
    12061 cgacattgcg tgatttgcgc atccgccaaa atgcccttgt taacagtcag gttcttaaat
    12121 tcctacctcc cggcccagca acggataaag acgttgagat cgttcggcag ggtgcgccaa
    12181 ctgacatgga taaccctgag acggtcgcaa gatggcttga tgcaatggca aaccttgagc
    12241 gacgaaacgc gcagtttaat gagtttaaag ctgagtggat gagcgcgaat ggcaatcctg
    12301 gacaatcgcg taatggcggt cagatattgg ggttggatgt taaaaaaggt gaatcattgg
    12361 ggagtgccgt taagcggtat atgtcattga atactgacgc agcgccagca caagattcga
    12421 caccttcagg agaaccacgg aatcaggttg gatcatatac ctcaaaatca ggcattcaat
    12481 ttacggtgga atgatgaaag taactgcaaa cggtaagaca tttaccttcc ctgatggtac
    12541 gagcacggaa gatattggca ccgccattga tgagtatttt gctggacagt cagcgccaac
    12601 acaacaaggt gttcagcaat cgccagcaga caactcactt gcatcaggat atgcacagct
    12661 tgccactcag cagaaggaag gactagatcg ctctgctgag caaggggctg ttttaggtgc
    12721 tgcaatgcgc gatgccgtta ccggtgaaag ccgaatgaca ccagaaatgg agagactgca
    12781 aaatgttggg tctgctccag agcttaatag cttaagcact gatgcgctgc gtgctggatt
    12841 ggggcagcta tttggttccg acgcttcaca ggagaaaata ctgcaaagta ttggcgggaa
    12901 aatccggaag gatgagaagg gaaattccat agtcaccctt ccttcagggg aatatgcact
    12961 taacaagcct ggtttgtcac cgcaggatat aacgtcattc ttggcaaatg ctcttgcatt
    13021 cactccagca ggtagagctg cgtctgttgt aggtgcaaca ctaaaatcag gcgctactga
    13081 tttagcttta cagggtgcca ctaagatcgc tggcggtgag aatgttaatc cagttcaaac
    13141 tgcaatttct gctggacttg gtggggtact gaagggtgta gaaaacaccg caagcgcagt
    13201 gtctcgctct gctatgggta agattgctcc tgaaaaacaa gctcagattg actttgccaa
    13261 gcagaacaac ttgccactga tgacaacaga tcttgtggaa ccgggaacaa atattggtaa
    13321 gcaagcacga gctatggctg agcgaatccc aatagccgga acaggtggga taagaaatgc
    13381 acagcaaaag gccagggaag atttagttag aacatttagc gataatgttg gcggaatatc
    13441 tgacgcacaa ctttaccaat cagctactcg tggtcagcag caatttattc aggctgctgg
    13501 caagaggtac gacaggatca tcagtttgat gggggatact cctgttgaca tcactggaac
    13561 agtgaaagca attgatgatc agatttccaa gttaactcgc ccaggagtat cgcaagaccg
    13621 ctcagctgtt tctgtccttc aacagtttag aaatgacatc accagcggtc caaataacct
    13681 gcaattagct agagaaaacc gcacaaactt acgtaagcgc tttatggcag cacctgacga
    13741 ggtcgataga gatacgctgg agaaagctgc gcagtctgtt tataacgcat acacaacaga
    13801 catgaaaaaa gcggttggcg caaaactagg tgcgaaggaa gcgcaaaaca tgtcgcgtgt
    13861 tgatcgttct tgggcaaagt tcaacgacat gatgagcaat acacgtgtcc aaaaagctat
    13921 tcagagtggt aaaacaacgc cagaagatgt cactaaacta gtattcagcc aaagcccagc
    13981 ggaaagggcg caactttata gattgcttga tgatagtggg cgtcaaaatg ctagagcagc
    14041 acttgttcag cgtgcaatgg ataaggcgac aagcgattca ggaaagctta gcgttgagaa
    14101 gtttattaat gaaatgaaaa ggaatcggaa gcaggctgag acgttcttca gaggagagca
    14161 tgggaaacag cttgatggga taatgaaata tcttgattcc accagacagg cagctactgc
    14221 tgccgcaagc ccactgacag ggcaaatggt agctggtcca gcagcgctga taacagctct
    14281 tgcgtctgtt acaaatccaa tgtttgcaaa agttgcggca gttggagctg gtatcggtat
    14341 ggctggcagg ggctatgagt cacgcgcgat gaggaacgca ttactaaagt tagcaaacac
    14401 gccaaaagga agtactgctt atgatagagc gatcagacgg gtatctgaaa ctcttacacc
    14461 tctaattcag gcttcaagtg agaaagccca gcagtaaaaa gttggttagc ggttgatggt
    14521 tgcttttttc gggtcatacc atctcggcca ttctttcagg aatgggaatg agtcaggtgc
    14581 gtggttcttt ttgtacgatt taagcagcct taaccgctca atcgcacact cataaacctc
    14641 ttgttgccct gtagtcatct cggtccatga aaggtgatcc attgataaga caacgttttc
    14701 agcttctttt atgagggcgt ttttatttct caccgcagca gcatggctga cagagcaatc
    14761 ctgccatatt cttaaaagcc aaatagccaa gcagatgaaa aaaatggtag atagcgatat
    14821 atacacacca acctccttag ttttgagcag gataccatga aaaaagttaa cattggaaac
    14881 gtaccaaaga tgctcgttcc gctctttgag agcggtacag ttgtgttttg tagagacttt
    14941 ccagaatggc aacgcctgca tcaaaaactt ggtgtggacg tgcaggactc gg
//
LOCUS       DAEVZA010000119        14796 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEVZA010000119 DAEVZA010000000
VERSION     DAEVZA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.119"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..784)
                     /gene="oppC"
                     /locus_tag="LAF92_003807"
     CDS             complement(<1..784)
                     /gene="oppC"
                     /locus_tag="LAF92_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBI9792740.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDG"
     gene            complement(799..1719)
                     /gene="oppB"
                     /locus_tag="LAF92_003808"
     CDS             complement(799..1719)
                     /gene="oppB"
                     /locus_tag="LAF92_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBI9792741.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(1805..3436)
                     /gene="oppA"
                     /locus_tag="LAF92_003809"
     CDS             complement(1805..3436)
                     /gene="oppA"
                     /locus_tag="LAF92_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBI9792742.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            3720..4016
                     /locus_tag="LAF92_003810"
     CDS             3720..4016
                     /locus_tag="LAF92_003810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792743.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(4174..4821)
                     /gene="ychE"
                     /locus_tag="LAF92_003811"
     CDS             complement(4174..4821)
                     /gene="ychE"
                     /locus_tag="LAF92_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBI9792744.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            5298..7973
                     /gene="adhE"
                     /locus_tag="LAF92_003812"
                     /gene_synonym="adhC"
     CDS             5298..7973
                     /gene="adhE"
                     /locus_tag="LAF92_003812"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBI9792745.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(8276..8893)
                     /gene="tdk"
                     /locus_tag="LAF92_003813"
     CDS             complement(8276..8893)
                     /gene="tdk"
                     /locus_tag="LAF92_003813"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBI9792746.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            9499..9912
                     /gene="hns"
                     /locus_tag="LAF92_003814"
     CDS             9499..9912
                     /gene="hns"
                     /locus_tag="LAF92_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBI9792747.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(10056..10964)
                     /gene="galU"
                     /locus_tag="LAF92_003815"
     CDS             complement(10056..10964)
                     /gene="galU"
                     /locus_tag="LAF92_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBI9792748.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(11166..12179)
                     /gene="rssB"
                     /locus_tag="LAF92_003816"
     CDS             complement(11166..12179)
                     /gene="rssB"
                     /locus_tag="LAF92_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBI9792749.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(12271..13176)
                     /gene="rssA"
                     /locus_tag="LAF92_003817"
     CDS             complement(12271..13176)
                     /gene="rssA"
                     /locus_tag="LAF92_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBI9792750.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            13289..13747
                     /locus_tag="LAF92_003818"
     CDS             13289..13747
                     /locus_tag="LAF92_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBI9792751.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            13797..14639
                     /gene="purU"
                     /locus_tag="LAF92_003819"
     CDS             13797..14639
                     /gene="purU"
                     /locus_tag="LAF92_003819"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBI9792752.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         4041 a         3641 c         3302 g         3812 t
ORIGIN      
        1 cgccatcact cagcaatgca ccccagctgc ttaacggctc ttgcgtaccc aaccccagga
       61 agctaaggaa agattcaaag aggatcatgc tgggcaccag aagtgatgcg tagaccacca
      121 ccacaccgag tacattcggc acaatgtggc gaataacaat gcccgatgtc gatacaccac
      181 caacttgtgc cgcctcaata aactctttgc gcttcagact cagggtttgc ccacgcacaa
      241 tacgagccat atccagccag gaaaccatgc ctatcgccac gaaaatcagc aggatgtttt
      301 gaccgaaaaa ggtcaccagc aaaatgacga agaacatgaa tgggaaggag ttgaggattt
      361 ccagcagacg catcattacc gaatccactt taccgcccag ataaccggaa agcgaaccat
      421 aaagtgtccc gacgaccaca gccaccagtg ccgcagcaac accgaccatg agtgagatac
      481 gcccgccaat cgcaacgcgc acaagcaggt cgcgaccgga tgagtcagta ccaaagtagt
      541 gaccggactc catatccggg gcgctggaca tcatcgccca gtcagtatcg tcataggcaa
      601 actgggaaag catcggtgcc aggattacaa ataacgcgat cagcaccagc actatcagac
      661 tggcaaccgc cgcacggtta tgcataaaac gtcgacgtgc gtcctgccac aagctgcgcc
      721 cttcgacctc cagcttttca ctgaaatttt ccagcgtctc gctgtttttc ttacttaaca
      781 tcatcgcgag ctccagtatc agtaacggat tttcgggtcg ataaccgcat atagcacatc
      841 gacaatggca ttaaacaaaa tggttaaagc accaaccagg atggtcaggc ttaacactaa
      901 ggaatagtca cggttcaatg cgccattaac gaacagttgt ccaatccccg gcaaaccata
      961 aatggtttcg ataaccatag aaccggtaat aatgccgaca aatgcggggc ccatataaga
     1021 gagcacgggt aacagagcag gttttaatgc gtgacgtaaa atgatccgcc gcataggtaa
     1081 ccctttcgcc cgggcagtac gaataaagtt ggagtgtaat acttcaatca tagagccacg
     1141 ggtaatacgc gcaatactgg cgatataagc gagtgacaac gccaccatcg gcaatatcat
     1201 gaatttaaga gccccaccat tccagccacc gcccggcagc caatgcaaaa tgatcgcaaa
     1261 tatcatgact aataatggcg caaccacaaa actggggata acaaccccgg tcattgccag
     1321 ccccatcacg gtatagtccc atttggtgtt ttgttttaat gcggcaataa cgccagcact
     1381 aacacccagt attaccgcaa ggaaaaatgc tgcggctccc agtttggcag aaacgggaaa
     1441 actggatgca accaggtcat tgaccgaata atctttatat ttaaacgatg gaccgaaatc
     1501 accgtgcgcc agttgtttca ggtagctgaa atactgtgtc atgatcggat cattaagatg
     1561 atatttcgct tcgatattgg ccatcacttc tggcggtaaa gtacgttcgc cggtaaaagg
     1621 gcttcccggc gcgaggcgca tcataaagaa cgaaatagta ataagaataa atagcgtcgg
     1681 aatcgcttcc agacagcgac gtagaataaa ttttaacatt gccctacctt ctggcctgtg
     1741 ccttctgtaa tgcgataaaa atcagacacc gtggagcagg acactcctgc cccacgtatt
     1801 gccattagtg cttcacaatg tacatattcc gggtataggt attatccagc ggatctttgc
     1861 cggtatagcc accaacccac ggtttcacca gacgcgcatt cacgtagtaa taaacaggaa
     1921 caatggccga atccttatcc agctgttgtt ctgctttagt gtacagagct gtgcgctgcg
     1981 cctcgtcagt cactttcagc gtttccgcca taatgctgtc aaaggccggg ctcttataat
     2041 gcgcggtatt catcgagctg ttcgaaagca tggtgttcag gaaggaagtt ggttcgttgt
     2101 agtcagcaca ccagcctgca cgggccacat caaaagtacc ctggtgacgg gtgtcgagga
     2161 acgttttcca ctcctggtta accagtttga cgtttacacc aatgtttttc ttccacaatg
     2221 aagaggcagc aatcgccagc tttttatgca gatcggaggt gttatacaac aggttgatgg
     2281 tcaacggttt gtctgcggta taacccgctt cagccagcag ttttttcgct tcttcgttac
     2341 gtttttcctg gctccagcca aaccattccg gctgagtcaa ttttgcgcca tcagtatacg
     2401 gtggagtgta accataggcg ggcatgtcgc cctgcgcttt cactttatta acaatgatat
     2461 cgcggtccat acctagtttc agtgcggtac gcacacgcac atcgttgaat ggcggtttct
     2521 ggttgttaat ttcgtaatag taagtgcaca ggtatggatc aacgtgaact tcgtccggga
     2581 tctctttttt cagcttctgg aacaattcga tcggcatgtt gttataagtc atgtcgattt
     2641 caccactacg gtagcggttg acatcggtaa cttcagaagc aataggcaaa taggttacct
     2701 ggttaataac ggttttcgcg ttgttccagt aggtcgggct gcgttcaaga acgattcgtt
     2761 cgtttacgac ccaatctttt aaggtatagg caccgttggt gacgatatta ccaggctggg
     2821 tccatttttc gccgaatttc tcgatagcgg cttttggcac cggtgaagtt gatgggtgaa
     2881 caagtaattt atagaagtac ggaacgggtt cacttaaggt gacttctaat gtgtgatcat
     2941 caatagcttt cacgccgaga tcggtaatcg gttttttccc ttcaagaatt tcatcaatac
     3001 cggcgatatg cccatattgc agataactgg catacggaga agcagtgttc ggatcaacag
     3061 aacgttgcca gctatacaca aagtcttgtg ctgtgactgg cgtgccatca gaccatttcg
     3121 catctttacg caaatggaag gtccagactt tcgcgtcttt attatcccag gattcagcga
     3181 cgccaggtgc tggatgaccg tcaagatcgc tgaccagtaa gccttcaaac aggtctcggc
     3241 tgatattaga ctccggaaca ccttcaattt tgtgcggatc taatgactga acttctgaac
     3301 cattgttacg taccagtgtt tgtttttccg ccagtgtgac gcctgcgggt acatcagctg
     3361 ccagcgcgac attccctgcc attagcgcag ccagaacgcc agctgctact aaacttctct
     3421 tggtgatgtt ggtcattgtt ttttggactc cctcattata attactggta ttaaccagca
     3481 tgtgtaatcc cggattggga ccctgcacag gctcggagac tttctgctgt caggttactt
     3541 tatcgttacg tattactatc accgacttta tttattctgg tgattcagac gatcacctta
     3601 tgtcgattcg tactgtgtct cccctgtcga gacacattat tctttcgcga cgctctttaa
     3661 ttgttaatta ttctcattac caataaataa tcgtctggcg gttgcccgga aagtaccaaa
     3721 tgcggttctt actcgccaat acattttgca aaattgttaa gcaattctct tttattgacc
     3781 gtatcagaat ctatagcttg catccatgcc ttaacaaaca tctttcaatt caatcaaata
     3841 gagattggct aagcatgtct atcttttctg attacagttc cagtagtgaa atgcacaata
     3901 atttaacaat tgattattat ttagcacttt cctctaccaa aggcagtgga attactaata
     3961 tcatttcaat tattcttcag caagcccagg attatgatgt ggcgaaaata acatgatggt
     4021 gaggagaagc agttaaggaa atccttataa ctaactgtaa tttgagaaga atatttttac
     4081 ctattccttt tgcttttgat aactgcgcgg gaacaaagta aaaataatga cgaagtaaac
     4141 ggagcatggc agctccgttt catttgaaag gaattaatta agcaggccgg ggaaaatccc
     4201 cttaatacca gtaacgataa attcaatccc caatgccatc agcaatagcc ccataatacg
     4261 cgtaatcacg ttgatgccgg tctggcgtaa aacccgtacc agccacggtg ccatgcggaa
     4321 caatccccag caacataaag cgaacaatgc aatagccaca aagaaaccaa acagatagct
     4381 aatgctgtga taacgcgtac cccagacgat ggtagaactg atcgcccctg gccccgccat
     4441 caacggcaac gccaatggca ccacaccaat gctttcacgt accgcggttt ctgatttttc
     4501 ttgcttgttc tgtttatcct cgccaagctt gccgctgatc atcgacatcg ctatcgtcac
     4561 caccaggatg cccccggcga tacggaacga atcaattgat ataccaaaaa gttgcagaat
     4621 cgtgtcgccg agaaaaagcg agatccacaa gataatggcc acagacaggt tggctgtaag
     4681 gttagttttg tttcgcgctg ccgctgtctg ataactggtc atgctgataa agacgggaat
     4741 aatccctacc gggttgacca gcgcaaataa cccgatgaaa aatttgaagt aaacgggaaa
     4801 atcaaaaaag gtctgaatca cggttagctc cgaagcaaaa gccggataat gttagccata
     4861 aataaggttg aaaagacgcg ctgacaatac gccttttgac agcatttttc acctcctaac
     4921 tacttaaaat tgctatcatt cgttattgtt atctagttgt gcaaaacatg ctaatgtagc
     4981 caccaaatca tactacaatt tattaactgt tagctataat ggcgaaaagc gatgctgaaa
     5041 ggtgtcagct ttgcaaaaat ttgatttgga tcacgtaatc agtacccaga agtgagtaat
     5101 cttgcttacg ccacctggaa gtgacgcatt agagataata actctaatgt ttaaactctt
     5161 ttagtaaatc acagtgagtg tgagcgcgag taagcttttg attttcatag gttaagcaaa
     5221 tcatcaccgc actgactata ctctcgtatt cgagcagatg atttactaaa aaagtttaac
     5281 attatcagga gagcattatg gctgttacta atgtcgctga acttaacgca ctcgtagagc
     5341 gtgtaaaaaa agcccagcgt gaatatgcca gtttcactca agagcaagta gacaaaatct
     5401 tccgcgccgc cgctctggct gctgcagatg ctcgaatccc actcgcgaaa atggccgttg
     5461 ccgaatccgg catgggtatc gtcgaagata aagtgatcaa aaaccacttt gcttctgaat
     5521 atatctacaa cgcctataaa gatgaaaaaa cctgtggtgt tctgtctgaa gacgacactt
     5581 ttggtaccat cactatcgct gaaccaatcg gtattatttg cggtatcgtt ccgaccacta
     5641 acccgacttc aactgctatc ttcaaatcgc tgatcagtct gaagacccgt aacgccatta
     5701 tcttctcccc gcacccgcgt gcaaaagatg ccaccaacaa agcggctgat atcgttctgc
     5761 aggctgctat cgctgccggt gctccgaaag atctgatcgg ctggatcgat caaccttctg
     5821 ttgaactgtc taacgcactg atgcaccacc cagacatcaa cctgatcctc gcgactggtg
     5881 gtccgggcat ggttaaagcc gcatacagct ccggtaaacc agctatcggt gtaggcgcgg
     5941 gcaacactcc agttgttatc gatgaaactg ctgatatcaa acgtgcagtt gcatctgtac
     6001 tgatgtccaa aaccttcgac aacggcgtaa tctgtgcttc tgaacagtct gttgttgttg
     6061 ttgactctgt ttatgacgct gtacgtgaac gttttgcaac ccacggcggc tatctgttgc
     6121 agggtaaaga gctgaaagct gttcaggatg ttatcctgaa aaacggtgcg ctgaacgcgg
     6181 ctatcgttgg tcagccagcc tataaaattg ctgaactggc aggcttctct gtaccagaaa
     6241 acaccaagat tctgatcggt gaagtgaccg ttgttgatga aagcgaaccg ttcgcacatg
     6301 aaaaactgtc cccgactctg gcaatgtacc gcgctaaaga tttcgaagac gcggtagaaa
     6361 aagcagagaa actggttgct atgggcggta tcggtcatac ctcttgcctg tacactgacc
     6421 aggataacca accggctcgc gtttcttact tcggtcagaa aatgaaaacg gctcgtatcc
     6481 tgattaacac cccagcgtct cagggtggta tcggtgacct gtataacttc aaactcgcac
     6541 cttccctgac tctggggtgt ggttcttggg gtggtaactc catctctgaa aacgttggtc
     6601 cgaaacacct gatcaacaag aaaaccgttg ctaagcgagc tgaaaacatg ttgtggcaca
     6661 aacttccgaa atctatctac ttccgccgtg gctccctgcc aatcgcgctg gatgaagtga
     6721 ttactgatgg ccacaaacgt gcgctcatcg tgactgaccg cttcctgttc aacaatggtt
     6781 atgctgatca gatcacttcc gtactgaaag cagcaggcgt tgaaactgaa gtcttcttcg
     6841 aagtagaagc ggacccgacc ctgagcatcg ttcgtaaagg tgcagaactg gcaaactcct
     6901 tcaaaccaga cgtgattatc gcgctgggtg gtggttcccc gatggacgcc gcgaagatca
     6961 tgtgggttat gtacgaacat ccggaaactc acttcgaaga gctggcgctg cgctttatgg
     7021 atatccgtaa acgtatctac aagttcccga aaatgggcgt gaaagcgaaa atgatcgctg
     7081 tcaccaccac ttctggtaca ggttctgaag tcactccgtt tgcggttgta actgacgacg
     7141 ctactggtca gaaatatccg ctggcagact atgcgctgac tccggatatg gcgattgtcg
     7201 acgccaacct ggttatggac atgccgaagt ccctgtgtgc tttcggtggt ctggacgcag
     7261 taactcacgc catggaagct tatgtttctg tactggcatc tgagttctct gatggtcagg
     7321 ctctgcaggc actgaaactg ctgaaagaat atctgccagc gtcctaccac gaagggtcta
     7381 aaaatccggt agcgcgtgaa cgtgttcaca gtgcagcgac tatcgcgggt atcgcgtttg
     7441 cgaacgcctt cctgggtgta tgtcactcaa tggcgcacaa actgggttcc cagttccata
     7501 ttccgcacgg tctggcaaac gccctgctga tttgtaacgt tattcgctac aatgcgaacg
     7561 acaacccgac caagcagact gcattcagcc agtatgaccg tccgcaggct cgccgtcgtt
     7621 atgctgaaat tgccgaccac ctgggtctga gcgcaccggg cgaccgtact gctgctaaga
     7681 tcgagaaact gctggcatgg ctggaaacgc tgaaagctga actgggtatt ccgaaatcta
     7741 tccgtgaagc tggcgttcag gaagcagact tcctggcgaa cgtggataaa ctgtctgaag
     7801 atgcgttcga tgaccagtgc accggcgcta acccgcgtta cccgctgatc tccgagctga
     7861 aacagatcct gctggatacc tactacggtc gtgattatgt agaaggtgaa actgcagcga
     7921 aaaaagaagc cgctccggct aaagctgaga aaaaagcgaa aaaatccgct taatcagtag
     7981 cgctgtctgg caatataaac ggccccttct ggggccgtat tttttgttta cccaaagcaa
     8041 cttttccata aaccgacagc attagccttc atcatatttg cgacgatgta taacgcctaa
     8101 acacagggat attgtacttt acaggtcaca agtcaacgtc ggtgcttaag agccctgtga
     8161 ggcgtatagc ggcgttaaaa aactgccgag aagggtatat agcccggaag aagtgcgtaa
     8221 aacgaactga caggataaaa gtgccctgct caccctgtca gtaaagaaat tcttattaat
     8281 cgtggcgatg cctttcctga atagccgtta atgagccgac ttgtaacgcc tctttatagt
     8341 gtttacggca tacagaaacg tatcgttcat taccaccaat taccacctgc tcaccttcgt
     8401 tataaggtct gcctgcttga tcaagacgca gcaccatgct tgctttacgg ccacaaaaac
     8461 agatggtttt taattcaacc agtttgtcgg accatgccag taagtattgg ctgccaataa
     8521 ataattcacc tcgaaaatcg gtacgtaaac cataacaaag tacgggtata tcgagttgat
     8581 cgacaacctc cgataattca tatacttgtt gtctggttaa aaactggcat tcatcaacca
     8641 gtacgcaatg aattgcctgc tgttcatgtt ccgcacgaat ctcatcaaat aatgatgaat
     8701 tttggttaaa taattttgca ggcgatgaca aacctatacg cgaactgact ttcccggcac
     8761 caaagcgatc atcaatttct gccgtatata cgacagtgcg catgccgcgt tcctggtaat
     8821 tgtatgaaga ttgcaacaat gctgtagact tacccgcatt cattgcggaa tagtagaaat
     8881 atagctgtgc catcagccac aggccctcaa tgatatgaaa taaagttggc tggagtttat
     8941 cataattcgt cagtttttca gctcaacagt atgcacagaa gtgggtatat gcgttctccc
     9001 ttacgaagcc ttgcataatc cttctgagct atcattacaa ctgcctcgct tgttataagc
     9061 gggtaaatga ctgctggtaa ctattcacaa tctttaacct gttgcgcaag taatagccct
     9121 ctgttgacct ccaggagata gtgcaatact aagtccatgc tcttattgcg actgttctac
     9181 ttttcatcat tcgcttaata gggaattctc gtaaacacaa ctaatacaga agactgaaag
     9241 gtcgtcagcc tacgataatc tccccataaa atgtgacatg aatcaggaag ttttaacctc
     9301 acgtgctgcg aaatcatcgg tgtaaatagg gctatatgcc gcgtcttttc tggctaattt
     9361 tatgaaaaga tatttattgg cggcacaaaa taaagaacaa ttttgaattc cttacattcc
     9421 tggctattgc acaactgaat ttaaggctct attattacct caacaaacca cccccaatat
     9481 aagtttgaga ttactacaat gagcgaagca cttaaaattc tgaacaacat ccgtactctt
     9541 cgtgcgcagg caagagaatg tacacttgaa acgctggaag aaatgctgga aaaattagaa
     9601 gttgttgtta acgaacgtcg cgaagaagaa agcgcggctg ctgctgaagt tgaagagcgc
     9661 actcgtaagc tgcagcaata tcgcgaaatg ctgatcgctg acggtattga cccgaacgaa
     9721 ctgctgaata gccttgctgc cgttaaatct ggcaccaaag ctaaacgtgc tcagcgtccg
     9781 gcaaaatata gctacgttga cgaaaacggc gaaactaaaa cctggactgg ccaaggccgt
     9841 actccagctg taatcaaaaa agcaatggat gagcaaggta aatccctcga cgatttcctg
     9901 atcaagcaat aatcttttgt agattgcact tgcttaaaat cccgccagcg gcgggatttt
     9961 ttattgtccg gtttaagaca atttaataag aataagctat aaaaaaacgg cgtcgattgc
    10021 tcaacgccgt ttcgtggata acaccgatac ggatgttact tcttaatgcc catctcttct
    10081 tcaagccagg ctttaaattc cgtgccaagg gtgttatgac gaataccgta ttcaacgaag
    10141 gcctgcatgt aacctaattt attaccgcag tcatggctct tccctttcat atgataggct
    10201 tccaccgttt ctttttcgat cagcatatca attgcgtcgg tgagctgaat ttcatcacca
    10261 gctcccggag gggtttttgc cagcaacggc caaatatccg cgctaagtac gtaacgaccc
    10321 acaatagcga gattagacgg cgcaacatcc gctttcggtt tttctaccac accaaccatc
    10381 ggtacgcttt cacccggcgc taattcaacg cctttgcaat ccacaacgcc atatgcggtc
    10441 acatcagcaa ccggttcaac catgatctgg ctatgacccg tttcatcaaa gcggcggatc
    10501 atctctgcca ggttatcctg tgacaaatcg gattcatatt catccagaat aacatcaggc
    10561 aaaataacag ctaccggttc atcacccact accgggtgag cacacaatac cgcgtgtccc
    10621 aggcctttcg ccagaccctg acgaacttgc ataatagtca cgtgcggtgg acaaatagac
    10681 tgcacttcat caagcagttg acgttttaca cgtttttcca gcattgcttc cagttcaaaa
    10741 ctggtatcaa agtggttttc aatagagttt ttagatgagt gtgtaaccag cacaatttca
    10801 gtaatgccag ccgcaataca ttcattcacg acgtattgaa ttaatggctt atcgacaagt
    10861 ggcagcatct ctttcgggat ggctttcgtc gccggcaaca tcctggttcc taatcccgca
    10921 acggggataa cggctttttt gactttcgta ttaatggcag ccatttaaat tctcctggac
    10981 tgttcgtgtt ttgaacgtgt tcatatttct gtatcgcatc ccagtatatc agcaccccga
    11041 aggggctaag ttctgaaaag gaggcgagtt agcataatta agacaattac ttatacgaat
    11101 ggcgacgata gtaccaccag ccgacattag caggtaatgc aaatttagcc cgcgttatcg
    11161 tttgctcatt ctgcagacaa catcaagcgc agtcgaccac cggttcccca tatttggcat
    11221 tgccaggcat cgcatcgctg gctcaattga ttcaaataag cgttacctaa agtgcctaac
    11281 ggaacaccat tactgatttg cacctggtgt tcgccggtat ttaacgtcgc attcagaccc
    11341 gcagaaacca gaatgagatt tttcagttcg cgatgataat agccaactaa tagcggaaac
    11401 tgccccggca gattggcctg acgaagtaaa tggtttacct gcttcaataa cgcgcccaac
    11461 tctggcaacc gttgattttg gtgtgcaagc tgttcctgta ataatccgtt aaacaatgcg
    11521 cgtaataaca aggcagcaag tacgccatta tgtccagctc gggtgacatc aaggcaataa
    11581 aatgccagat cgttttccga aagtgcggca atatcaagca ccaggccggg tttgtccgcg
    11641 gcaaccaatt gacgataatt aacccggcaa tgggaaatca cctgctgaac cggcggttgt
    11701 agttcctgta ataatttcgc cgctgcggca gggttatcaa ccattgcatc ccagtcgcga
    11761 aaaagccttt cctcttcctc aacgcgcgaa ttaaacatgc tgggatagag acaggcaaaa
    11821 accatctcgc gcaagcgatt cagatcttta actggtttca gcaaaacatc ttcaacgccc
    11881 agacgtaacg ctttggcaat atctgccata ttttcagtgg cagatatcac cagaactggg
    11941 gtctggtcgc ctctgttacg tatatgctcc agcagtttaa gcccgttcat tcgtggcatc
    12001 gcgatatcac atatcatcag gtctggagtg aaacctccca gcaactcaag ggcatccacc
    12061 ccatcagccg ccagtaccgt tgtcgctccc aatgaggaaa accatgaatc cagaagcgag
    12121 cgaaatacct gctcatcttc aacaatgaga atctgttttc cgaccaatgg ctgcgtcatg
    12181 ttctctcccc tgactggctt tactcaatag tggcatgcta ttgccacctg cgcctgtcag
    12241 aattaactta agtgtagata aaaattctgg tcaaatgttg gtgcgtacca acggcaaaag
    12301 ttcgtccatt ttcctttcca ctgccagctg tccggccgca atggcagcgt gcgcgcgatg
    12361 gaaatcaagc gtagatattt gcgggcaaac aggttgaatc agaatatcgg gcggatcacc
    12421 tgccatgcgg ttccttttaa ggcggttctc cagcacctgg atagaagtgg tcataatctc
    12481 tgttgccgtt ggcgctgtca ccgcccgacg tgtcgttatg ctgcctaacc tttctttcag
    12541 acgcgcatgc cacggcagag aatcaccatt ctcgctattt tcttcactga cattaaagga
    12601 gagcaaatct tgttgcatca aatgagcatc gtgctgcagg tcaaccgcta tcacaatatc
    12661 agcccccaat gcacgcgtga gggaaatagg aattgggtta acgactgctc catcaaccag
    12721 ccagtagccg ttatgtgcaa caggtgccat gagtcctgga atactgcatg atgcgcgaat
    12781 agcaagatgg agatcgcctt cagtaaacca taattcacgt cccgtactta aattggtggc
    12841 aacagccgca aagcgacggg aacaattttc gatctctgtt tccggcatta tttcgcggta
    12901 ttgattgaag acacgctcgc cgcgcagtaa cccaccgcgc tgccaggaga gatccatcag
    12961 gcgtaaaaca tcccaataac tgaaagaggt cacccaatct tccagcgcag ataatcgatc
    13021 gcatgcatag gcagcgccca ccagcgaacc aattgaacat cctgcaacga tatcaatttc
    13081 aatacccact ttttttagcg cattaataac gccaatatgc gaccaacctc tcgccgcgcc
    13141 agatcccaac gccagcccta tttttatctt tctcattatt cccgcaagat tcccctggaa
    13201 tgcaatcgta gccacatcgc tactgctcgg ttaacatagt gctactcagg cggttaatac
    13261 gccgttattg tttccaggga gattatttgt gtctcagctt tgtccctgtg gtagtgctgt
    13321 cgagtatagc ctatgttgcc acccttatgt gtctggtgaa aaggttgcac ctgatccaga
    13381 acatctcatg cgttcgcgtt actgcgcttt tgtgatgcaa gacgcagatt atttaataaa
    13441 gacctggcat ccctcttgtg gagcagcggc gttacgtgcc gaattgatgg ccggatttgc
    13501 gcatactgaa tggttgggat tgaccgtatt tgaacattgt tggcaggacg ccgacaatat
    13561 cggatttgtc agttttgttg ctcgatttac cgaaggtggt aaaacaggtg caataattga
    13621 acgttcgcga tttttgaaag aaaacggtca atggtactat attgacggta cacgtccgca
    13681 gtttggtcgc aacgatccct gcccttgtgg ttcaggtaaa aaatttaaaa agtgctgcgg
    13741 ccaataatgg ttgacggtac ggtttagcaa acactctcaa caaggttttt ccagcaatgc
    13801 attcactcca acgtaaagtt ctgcgtacta tttgtccgga ccaaaaaggt ctgatcgcac
    13861 gtattaccaa tatttgctac aagcacgagt taaatatcgt acagaacaat gaatttgttg
    13921 atcaccgtac cgggcgcttt tttatgcgca cggaactgga agggattttt aatgattcca
    13981 ccctgctggc ggatctcgat agcgcattgc cagaaggctc cgtgcgtgag ctgaatcctg
    14041 ccggtcgtcg ccggatagtg attctggtca ctaaagaagc gcattgcctt ggcgatttgt
    14101 tgatgaaagc caattacggc ggcctggatg tcgaaatcgc ggcagttatt ggtaaccacg
    14161 atactttacg ttctctggtt gagcgttttg atattccgtt tgagctggta agccatgaag
    14221 ggttaagccg caacgagcac gatcaaaaga tggcggacgc cattgatgct tatcaacctg
    14281 actatgtggt gctggcgaag tatatgcggg tattaacgcc ggaatttgtg gcacgcttcc
    14341 cgaataaaat catcaatatt caccattcat tcctgcctgc gtttatcggc gcacgtcctt
    14401 atcaccaggc ctatgaacgt ggtgtgaaga ttattggcgc aaccgctcac tatgtgaatg
    14461 acaatctgga cgaaggccca atcatcatgc aggacgttat tcatgtcgat catacctaca
    14521 cagctgaaga tatgatgcgc gcaggtcgtg acgtcgagaa aaacgtctta agtcgtgcgc
    14581 tatacaaagt actggcacag cgcgtctttg tttacggtaa tcggacgatt attctttaat
    14641 cgccagcaaa aataactggt tacctttaat ccgttacgga tgaaaattac gcaaccagtt
    14701 catttttctc aacgtaacac tttacagcgg cgcgtcattt gatatgatgc gccccgcttc
    14761 ccgataaggg agcaggccag taaaaagcat tacccc
//
LOCUS       DAEVZA010000120        14783 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEVZA010000120 DAEVZA010000000
VERSION     DAEVZA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.120"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1108
                     /locus_tag="LAF92_003820"
     CDS             <1..1108
                     /locus_tag="LAF92_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBI9792753.1"
                     /translation="FSDHLYENDKHYHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNV
                     SLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEEERFNLFISIPF
                     YWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDT
                     SLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDA
                     PGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQGNRQIIAPY
                     RGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDE
                     IPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1121..2079
                     /locus_tag="LAF92_003821"
                     /pseudo
     CDS             1121..2079
                     /locus_tag="LAF92_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(2118..3017)
                     /gene="nhaR"
                     /locus_tag="LAF92_003822"
     CDS             complement(2118..3017)
                     /gene="nhaR"
                     /locus_tag="LAF92_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBI9792754.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3083..4249)
                     /gene="nhaA"
                     /locus_tag="LAF92_003823"
     CDS             complement(3083..4249)
                     /gene="nhaA"
                     /locus_tag="LAF92_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBI9792755.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            4835..4987
                     /gene="mokC"
                     /locus_tag="LAF92_003824"
     CDS             4835..4987
                     /gene="mokC"
                     /locus_tag="LAF92_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBI9792756.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(5091..6221)
                     /gene="dnaJ"
                     /locus_tag="LAF92_003825"
     CDS             complement(5091..6221)
                     /gene="dnaJ"
                     /locus_tag="LAF92_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBI9792757.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(6310..8226)
                     /gene="dnaK"
                     /locus_tag="LAF92_003826"
     CDS             complement(6310..8226)
                     /gene="dnaK"
                     /locus_tag="LAF92_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBI9792758.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8603..9007
                     /locus_tag="LAF92_003827"
     CDS             8603..9007
                     /locus_tag="LAF92_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBI9792759.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            9033..9746
                     /gene="msyB"
                     /locus_tag="LAF92_003828"
     CDS             9033..9746
                     /gene="msyB"
                     /locus_tag="LAF92_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBI9792760.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            9895..10461
                     /gene="satP"
                     /locus_tag="LAF92_003829"
     CDS             9895..10461
                     /gene="satP"
                     /locus_tag="LAF92_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBI9792761.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(10496..11083)
                     /gene="mog"
                     /locus_tag="LAF92_003830"
     CDS             complement(10496..11083)
                     /gene="mog"
                     /locus_tag="LAF92_003830"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBI9792762.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(11198..12151)
                     /gene="tal"
                     /locus_tag="LAF92_003831"
     CDS             complement(11198..12151)
                     /gene="tal"
                     /locus_tag="LAF92_003831"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBI9792763.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            12430..13860
                     /locus_tag="LAF92_003832"
     CDS             12430..13860
                     /locus_tag="LAF92_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBI9792764.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            13930..14706
                     /gene="yaaA"
                     /locus_tag="LAF92_003833"
     CDS             13930..14706
                     /gene="yaaA"
                     /locus_tag="LAF92_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBI9792765.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
BASE COUNT         3733 a         3673 c         3509 g         3868 t
ORIGIN      
        1 attcagcgac catctttacg aaaatgataa acactatcat cagagtgatt atgatgattt
       61 ttatgatatt ggcagaaaaa atagcctttc tgctaatatt atgcaacctt tatccaataa
      121 tctgggaaat gtatcattaa gtgcgctttg gcggaattac tgggggcgaa gtggaaatgc
      181 taaagattac caattcagtt actccaataa ctggcaacac attagttata ctttctctgc
      241 cagccaatct tatgatgaaa ataataaaga agaggagcgt tttaatctgt ttatctccat
      301 tcctttctac tggggggatg atattgccaa aacacgtcac caaattaact tatcgaattc
      361 gacctcattt tccaaagatg gctattcctc caacaatact ggaattactg gcatagccgg
      421 tgaacatgat cagttaaatt atggtatata tgttaatcag caacaacaaa ataatgatac
      481 ctcgcttggt acgaatttaa gctggagaac tcccatcgcc ataatagatg gcagctatag
      541 tcattctaaa aacgcctggc aaagtggtgg aagtattagt agtggattag ttgtctggcc
      601 cggtggtatt aatatcacta accagttatc cgatacattt gcaattctgg atgcgcctgg
      661 gttagaaggt gcacatatta atggacaaaa atacaatcga acaaacagca aaggccaggt
      721 tgtttacgac ctgataatac ctcatcgtga aaaccatctg gtacttgata tagcaaacag
      781 tgaaagtgaa acagaattgc agggcaatcg tcaaattatt gcaccttacc gtggagcagt
      841 ttcttatgtg cagtttacaa ctgaccaacg taagccctgg tatatacagg cactgcgtcc
      901 cgatggttcg ccattaacct ttggctatga cgtactggat ctccaggaaa ataatattgg
      961 agtcgttggc cagggtagtc gcctttttat tcgcgtagat gaaattccca ctggaataaa
     1021 agttgctctc aatgatgaac agaacttatt ctgtactatt acttttcaac acgttatcga
     1081 tgaaaacaaa acatatatat gccagtaaaa ggagtttact atgaagtggc ttttattaat
     1141 caccttgagt ctatattctt ttattgtgca aagtgctccc tgtgcattga ccaatgtcgg
     1201 tgaacaacgt ggaacatata tactcaagcc actgagtatg aaaggtaatc taaccgccga
     1261 aaaattgaaa acaccggaaa tatcattgag tgtacagact tgcaagagac aaccaacgcc
     1321 attaaagttc attttttaac acagaacgct ttaaggttta aagtcggaag tcgtcattat
     1381 attattaatt tcccctttga atctgacggt aaaaaaatag agcttccagg gaagtcatct
     1441 tataatttag aagaaatgct atctgcgata aatttcacca ttccatattc tatcgcatct
     1501 gtatcagata gttctaacac cgcagacatt tttcctggaa ctccttttcc tttgttgttc
     1561 gaaattgaag tgagttcatg tacagggaat aacactaatt gtaatagcgt ccgctttaat
     1621 tataatttaa atactatttt acaggtaaat ttaatgacct gtagttttga agatcaatcc
     1681 atcgacactg gacgatttgt actgtctcgt atcaataatg aaaattatca gtttcatcat
     1741 ataagatttg actgcatcca gggcgagcaa ggtgaattag tttttgaacc cagtgatgtt
     1801 gaatattact ttgaaccagt gacgatacag gagtcaggaa ccagcatatt aaaaaatgag
     1861 ttagaaaaca gtgaggacgg tgcgggtcaa ataggctttc aactctccaa tgatggtacg
     1921 aatgaaatcc agtatggtaa aagcaattac tactcttttc agcatcccca tgaaggaagt
     1981 aatcagattc ctctatttat tcgcccgcgt acatatggaa ataatgtctc aagcgggcaa
     2041 ataatgagcc gagtcaaaat cgtggttatg tacaactaat ctatctcccc caccttaact
     2101 ttggggggag gtgccgatta acgcgccgct ggactaaaaa gcgcagaata atccgtattg
     2161 cagattcgct gtaccgccgg gtgctgaatc atccgctcag caaaaatagc atggtactct
     2221 tccatcacat tctcgacgcg accaatttct acgacagttt tatcggcata aaagtcatat
     2281 gcataaagcg ttggggcaac gaagattgca ttgtgcatcg caccaaaagc tttcatcaac
     2341 gcggcatcat caaactcgcc gaggatttct acgtttaatc cctgggagtt aaaccagtta
     2401 agcaatttgc gccctaacat tgaacgtcgc ccaggaatca aaagtcgccg ttcttccaga
     2461 caagccggga acggtttttc tggtggtgga tttgtacacc agaaactcac gccacattcg
     2521 ccaattctca cggagaacag gccttcctgc tgcgtagagt ctatcggaca gtcagaaatg
     2581 atcatatcca gtttatgctg acttaattgc tccagcagca tttcgtgggt ggattcgaag
     2641 cagcgaagat gaatgggctc gccttctacc actgcggcgt taagtacgct actgaccagg
     2701 cgtttggaaa gtgcatcagc cacaccaacg tcaaacaata aattggattc tttgcgatag
     2761 ttcacaatat ccagcatttc ctggcttaag gtgaacattt tatcggcata gcgatagacc
     2821 agttctccca gctcgctggg ttcgagacca cgtcccttgc gtttaaataa tttgccttgc
     2881 aggcgctctt ccagcgctcg aatctgtccg gtaatggttt gtggagttaa ataaagcgcc
     2941 tccgctgcgc caaccacgga accttcttta tagacatgcc agaagtaata caagtggttg
     3001 taattgatat gagacatgct catttctctc cctgataaca atgaaaaggg agccgtttat
     3061 ggctccccgg taaaccgtcc tgtcaaactg atggacgcaa acgaacgcgt aaccagctgt
     3121 atccaattac cgccgaagag atagaaccga ccaggatacc gagtttcgcc cagttaatca
     3181 gttctggatc tacgctacca aaggccaggc tggcaataaa gatagacata gtaaaaccga
     3241 taccgcacag gatccccacc gccataattt gctgataagt cgttccctca ggcagatgcg
     3301 ccagtttcaa acgcagcgcc aaccagcaga acagactaat ccccagcggt ttgccaatca
     3361 gcaagccagc gatgatcccc aatggcagaa tggaggtcaa gccatccagc gtgacacctt
     3421 gcagtgaaac gccagcatta gcaaatgcaa acagcggcaa aatcagatac gccacccacg
     3481 ggtgtaacac atgctccagt cgcttcgctg gagaacgccc atgcttctct ttcaaaggaa
     3541 taaagaagcc gacaattacc cccgccaggg ttgcgtgaac ccccgatttc aacaccgcag
     3601 tccacaacac tacgccaacc agaatataga cgcccgtgcg gcgtacacca cacagattca
     3661 ataccgcgag taccgcaatt gctacagccg cgacgccaag agaggccatc gataagtcat
     3721 tagtgtagaa caatgcgatg ataatgatgg ccccaagatc gtcgataata gccagagcca
     3781 tcaaaaagat cttcagcgct aacggaacac gacttcccaa cagcgccagc acaccaagtg
     3841 caaaggcaat gtcagtagcc gccgggatcg cccacccttc gcgggtaatc ggatcggcat
     3901 agttaaaggc cagatagagt aatgccggaa caatcatccc accaatagcg gcgataactg
     3961 gaaatgcggc ctggcgtaag ctggctagcg atccttgcat cagttcacgt ttaacttcca
     4021 gaccgactaa caggaaaaat accgccatca gcgcgtcatt tatccataac agcatgtttt
     4081 tgttgatttc gagtgaacca acccggagct gaaccggcgt ctccagaaag tcgtgatacc
     4141 atccactggt tgcgccgcta ttggccataa tcatcgccag gatagcggca atgataagaa
     4201 taatgcctcc cgaggcatca ctgctaaaga atcgatgcag atgtttcact ttttatttct
     4261 ctttcaggtg aatagatcgt ttttactatt ttaccccgca caaatcatcg ttaaaatcag
     4321 attatagtgt aggaataact cgttttttac gagcaattaa aggatttgcg ccatcagcgg
     4381 tgagatgagt ttttgacggc tgtaagcgct aacgggtgac cctaagccct gataatcata
     4441 gcttaaggtc aggatttctg agggattaat taggaaaagt aataattaat ttcttgtcag
     4501 tcggcgttat ttgttaggac aatatttcaa aaataatcca gcttgtcgtc aataattgcg
     4561 ataaagagat aaattaaagt taaataatta aatcattaaa aaattacgcc acaaaaatgc
     4621 gaggcgtctt caggattaga atcgataaaa cagaatgggt taaagagagc gatataatag
     4681 cggcgggtgc ttgaggctat ctgtctcagg cattagctga acggcagata gagaaaagcc
     4741 ccgagtgata ttttactatc aacccgaggc cccctatatg ctgaacacat gtagagtgcc
     4801 tcttactgac cgtaaggtca aggagaagag agcaatgaag cagcataagg cgatgattgt
     4861 cgccctgatc gtcatctgta tcaccgccgt agtggcggcg ctggtaacga gaaaagacct
     4921 ctgtgaggtt cacatccgaa ctggccagac ggaggttgct gttttcacgg cttacgaatc
     4981 cgagtaagtg caacggcggg gagtgatccc cgccactttt caggtgctcg catatcttca
     5041 acgcacccta attttaccca agcctgccca cgggcaggct tttggggagg ttagcgggtc
     5101 aggtcgtcaa aaaacttctt cacaccatca aagaagctct ttgagcgcgg gctgttgtgc
     5161 tcaccggttg ggccaccgaa gctttcttgc agctcttgca gcagctgttt ctgcttctcg
     5221 ttcaggccta ccggtgtttc gacgacaacg cggcacagca aatcaccctg tgcgccaccg
     5281 cggacagact tgacgccttt accgcgcata cggaaaagct taccggtctg ggtttcgcca
     5341 ggcactttca gtttgacgcg accatcaagg gtcggtactt cgatttcgcc acccagcgcc
     5401 gccatagcga agttgatcgg gacttcgcaa tagaggttgt tgccttcacg ctcgaaaatc
     5461 gggtgctgtt taacctgaac ctgaacgtac agatcgcctg ccggtgcgcc atgctcgccc
     5521 gcttcacctt cgcccgcaag acggatgcgg tctccagtgt ccacccctgc cgggatttta
     5581 acggacagcg ttttgctgcg ctcaacacga ccatgaccat gacatttgtt gcacggatct
     5641 ttgatcagcg taccgcggcc ctgacagtgt ggacaggtct gctgtacagc gaagaatccc
     5701 tgacgcatct gcacctgacc agaaccatga caggtcggac aggtctgcgg ctgtgtacct
     5761 ggttttgcac cgctaccgtg gcaaacgtca cactcttcca gagtcggaat gcggatctct
     5821 ttggtcacgc cacgtacagc ttcttcgagg gtgagctcca tgttatagcg taaatcagca
     5881 ccgcgcgccg cacgttgacg accacgtccg ccgccaaaaa tatcgccgaa aacgtcacca
     5941 aaaatatcgc tgaagtctgc gccgccgcca aaaccgccgc cgcccatgcc gccttgctca
     6001 aacgcagcat gaccatactg atcgtatgcc gcacgttttt gcgagtcggt cagaacttca
     6061 taagcttcct tgatctcttt aaatttcgcc tcggcctctt tgtcaccctg gttacggtcc
     6121 gggtggtatt tcatggccag gcgtttgtag gcctttttga tttcacgctc ttccgctgtt
     6181 ttggaaacgc ctaaaatctc gtaataatct tgcttagcca tcttttttaa attgccccta
     6241 gatgaatgca cgggcggaga ggaaattccc cttcgcccgt gtcagtataa ttacccgttt
     6301 atagggcgat tattttttgt ctttgacttc ttcaaattca gcgtcgacaa catcgtcatc
     6361 tttcgcgttg tttgcagaag catcagcacc ggcagtctgc tgctgggcat gttgctgctg
     6421 ggcgatttcc atcagtttct gggaaacctg tgccagctcc tgcattttcg cttcgatagc
     6481 ggctttgtct tcacctttca gagcagtttc cagtgcagtc agtgcagact cgatagcagt
     6541 tttgtcgtca gccggcagtt tgtcgcctgc ttcttcaacc tgcttacggg tgctgtgcag
     6601 cagatggtcg ccctggttgc gagtctgtac cagctcttca aacttacggt cagcttcggc
     6661 gttagcttct gcgtcgcgta ccattttctg gatttcatct tcgttcagac cagaagaagc
     6721 cttgatggtg atcttctgct ctttaccgct gtttttatct ttcgcggaaa cgtgcaggat
     6781 accgtcagca tcgatatcga aggtaacttc gatctgcggc atgccgcgcg gtgccgggtt
     6841 gataccatcc aggttgaact gacccagaga tttgttatca gccgcacgtt tacgttcacc
     6901 ctgcagcaca tggatggtta ccgcagactg gttgtcttca gcggtagaga acacctggct
     6961 gtgcttggtc gggatagtgg tgtttttcgc gatcagcgtc gtcatcacac cgcccatggt
     7021 ttcgataccc agagacagcg gggtaacgtc cagcagcagt acgtctttca cgtcaccagt
     7081 cagaacacca ccctgaacag cagcaccgat tgctacagct tcgtccgggt taacgtcttt
     7141 acgcggctct ttaccaaaga actcagcaac tttcttctga accattggca tacgagtctg
     7201 accaccaacg aggataacgt cgtcgatatc agatacggac aggccagcgt cctgcagtgc
     7261 aactttcagc ggctcaatgg aacggtttac cagatcttca accaggcttt ccagtttcgc
     7321 acgagtcact ttgatgttca tgtgtttcgg accggtcgca tctgcagtga tgtacggcag
     7381 gttaacgtcg gtctgctgag cggaagacag ttcgattttc gctttttctg ccgcttcttt
     7441 caggcgctgc attgccagcg gatcgttgcg caggtcaatg ccctgatctt tcttgaattc
     7501 ttcaaccagg tagttgatca gacggctgtc gaagtcttca ccacccaggt gggtatcacc
     7561 gttggtagcc agaacttcga aggttttttc gccgtcaact tcgtcgattt cgataataga
     7621 aatatcgaaa gtaccaccac ccaggtcata aaccgcgata gtacggttgc cagtaccttt
     7681 gtccagaccg taagccagcg cagctgcggt cggttcgttg atgatacgtt ttacttccag
     7741 accagcgata cggcctgcgt ctttggttgc ctgacgctga gcatcgttaa agtatgccgg
     7801 tacggtgata acagcttcag ttaccggttc acccaagtaa tcttcagcgg ttttcttcat
     7861 ttttttcagc acttcagcag aaatctgcgg cggtgccatt ttctggcctt taacttcgac
     7921 ccatgcgtcg ccgttatcag cagcaataat tttgaacggc atgatggaaa catcacgctg
     7981 tacttcttcg tcctggaagc ggcggccaat caggcgttta atcgcaaaca gggtgttttg
     8041 cgggttcgtc actgcctgac gtttagccgg ctgaccaacc agagtttcac catcctgggt
     8101 ataggcaatg atagaaggcg tggtgcgatc gccttcggcg ttctccagca cgcgaggagt
     8161 ggtgccatcc ataatcgcta cacaagagtt ggtagtaccc aggtcgatac caattatttt
     8221 acccatctaa acgtctccac tatatattcg gtcatcatgt ggttgtgagt ctgtaatagg
     8281 ggcgaaacgt ctggtttcaa ctgcccaatt tcattttttt gcagactcac tcactgcggt
     8341 tgactactaa atggggtcgt aacccacgtc atcaaggggg agatgcaaaa attttttgtg
     8401 cttttgcgca gaaatttcgc gatatagcag gacaagacgt gaaactgaca gcaattttat
     8461 tcatataaag aatgaatcgc agccaagaca ttcatcaaaa aattgtaata aaaagaaaac
     8521 attacgcacc tgaatcttct ctttatcagc agtaaactag tgggtatcca tccccctacc
     8581 tctccccact aagagaatcc ttatgaaatc cgtttttacg atttccgcca gcctggcgat
     8641 tagtctgatg ctgtgctgca cggcgcaggc aaacgaccat aaaatcctcg gcgtcattgc
     8701 aatgccgcgt aacgaaacca acgatctggc gttgaaactt cctgtttgcc gaattgttaa
     8761 acgcatacaa ctctctgccg accatggcga tttacagtta agcggtgcat cggtttattt
     8821 caaagccgcc cgtagcgcca gtcagagcct gaatattcct tcagaaataa aagaagggca
     8881 aaccactgac tggatcaaca ttaacagcga taacgacaat aaacgctgcg tctcaaaaat
     8941 caccttttcc ggtcatacgg tgaactcatc ggatatggcc acgctgaaaa ttatcggcga
     9001 cgactaacgc cagaatatag aagccacaaa aaatgaatgt taattacctg aatgattcag
     9061 atctggattt tctccagcat tgcagtgagg aacagttggc aaatttcgcc cgattgctca
     9121 cccataatga aaaaggcaaa actcgcctct ccagcgtact gatgcgcaac gaactgttta
     9181 aatcgatgga agggcatccc gagcaacatc gccgcaactg gcagctgatt gctggagaat
     9241 tacagcattt tggtggcgat agtatcgcca acaaactgcg cggacacggt aaattgtatc
     9301 gggccatttt gctcgatgtt tcaaaacgat tgaagctgaa agccgacaaa gagatgtcta
     9361 cgtttgagat tgagcaacag ttactggaac aatttctgcg taacacctgg aagaatatgg
     9421 acgaggaaca taagcaggag tttctgcacg cgatcgatgc cagggtgaat gagctggaag
     9481 agctgctgcc gctgctgatg aaagacaaat tattggcaaa aggcgtgtcg catttgcttt
     9541 ccagccaact gacccgcatt ttacgcaccc acgcagcaat gagcgtactt gggcatggtt
     9601 tgctgcgcgg cgcggggctg ggaggccctg taggtgcggc actaaatggg gttaaagcgg
     9661 tcagcggcag cgcctatcgc gtgacgattc cagccgtact gcaaatcgcc tgcctgcgcc
     9721 ggatggttag cgccactcag gtctgatacc ggaaaccggg gaaaattttc attttcccct
     9781 caaaagatcg tagacactgc cccactggct gattattatg ccgcgccctg aaaacactac
     9841 agttattcag ggaaattatt tcaccattca ttcgatgatg atttttgagg aattatgggc
     9901 aacactaagt tggctaatcc ggcaccgctg ggcctgatgg gcttcggcat gaccaccatt
     9961 ctgcttaacc tgcacaacgt gggttatttc gctctggacg gtattattct tgccatgggc
    10021 attttctacg gcggcatcgc gcaaattttt gctggtctgc tggagtacaa aaaaggcaac
    10081 actttcggtt taaccgcatt cacctcttac ggttctttct ggctgacgct ggttgcgatt
    10141 ctgctgatgc cgaaactggg tctgaccgat gcgccaaatg cacagttcct tggtgtctac
    10201 ctgggtctgt ggggcgtatt tacgctgttt atgttcttcg gcacgctgaa aggcgcacgc
    10261 gttctgcaat tcgttttctt tagcctgacc gtgctgtttg ccctgctggc gatcggtaac
    10321 attgccggta acgccgcaat catccacttt gccggctgga ttgggctgat ctgcggtgcc
    10381 agcgcaatct atctggcgat gggtgaagta ctgaacgagc agtttggtcg caccgttctg
    10441 ccgattggtg aatcccacta attgttttga gatccccccg ctcgggggga tttttttatt
    10501 cgctaacgtc gcgtcttgca ctcttcggtc tgaatgctgc aaccacttcc ggtgccgttt
    10561 caacgtatgg cccttccagc aactgaatgc agtacggtac gctggcaaaa ataccgtgta
    10621 ccacaacgtt acccgcagcg tccttcacac cttccagcgt ctctttaata gacttcggct
    10681 gaccgggtaa gttaaggatc agcgcctgtt tgcgaatcac accaacctga cgcgaaagga
    10741 tcgcagttgg tacaaaatgc aggcttatct ggcgcatctg ttcaccaaag ccaggcatct
    10801 cgcggtccgc tactgccagc gtcgcatcgg gcgttacgtc acgacgcgcc gggccagttc
    10861 cgcccgtggt gagcaccaga tggcaactca tttcatccac tagctcacac aacgtttgct
    10921 cgatgatcgc ctgctcatcg gggattaagc gggtttccag ttcaaacggc gtggttagcg
    10981 ccgatgtcag ccattcttcc agcgcaggga tgcctttatc ctgataaacg ccgctggatg
    11041 cgcgatcaga gatggaaact aagccaatac gtaaagtatt catcaagatt tttccgttta
    11101 aacagtctcg ttaaacagaa tgatacactg cgaagggagt gacagacagg cttcagaaga
    11161 ggtagcgtga ccgacttccc ggtcacgcta agaatgatta cagcagatcg ccgatcattt
    11221 tttccagttt ttcctggtca atagcaaact tacggatacc ttccgccagt ttatctactg
    11281 ccattggatc ctggttgtgc tgccacagga actcggactc agtgatacgc gccggacgcg
    11341 ctttcacttc gccggtgtaa gacagtttac gttcgatagc cccttcgctc tcagccagct
    11401 ctttcagcag tgccggtgcg atggtcagac ggtcgcagcc tgccagttcc agaatttcgc
    11461 cgatgttacg gaagcttgcg cccataacca cggtttcata accgtgctct ttgtagtact
    11521 ggtagatttc agatacagaa accacgcccg gatcttctgc cggagcgtac tctttcttat
    11581 cagtattcgc tttgtaccag tcaagaatac ggccaacaaa cggcgagatc aggaacacgc
    11641 ccgcttccgc acaagcacga gcctgagcga aggagaacag cagggtcagg ttacagttga
    11701 tgccttcttt ttccagctgt tctgcagcac ggataccctg ccaggtagaa gccagtttga
    11761 tcagaatacg atcgttgcta ataccagcat cgttgtagag tttgatcagg cgttttgctt
    11821 tcgcaattga cgcttcagtg tcataggaaa gacgcgcatc aacttcagtt gagatacggc
    11881 ccggaaccag tttcaggatt tccagaccaa tatttactgc cagtttgtcg gtcgcgtcca
    11941 cgatctgctg cgcgcgatcg ttgctctgct gtttcgccca ggcgacagca tcatcaatca
    12001 acttacggta ttccggaatc tgcgctgcgt taagaatgag agaagggttg gttgtggcat
    12061 cctgcggttg atacagcttc attgccgcga tgtccccagt gtcggccact acggtggtgt
    12121 actgacgaag ggaggtcaat ttgtccgtca tgatagtatt tctctttaaa cagcttgtta
    12181 gggggatgta accggtctgc cctgatgata tcacgacgca acgtcagcgc aaccgccgac
    12241 aagacggtta tcgccaggcg agactgtttc ggatttctga cactgcgttg atatcgcccg
    12301 ccatttttat acaaaacctc atgtatgcta cgcagaagtt atcaagtacc tcgtagcgta
    12361 tatacttctt aaacaattgg taacgtttac acaggaaagt catcgcgacc ggcaataaga
    12421 gggatatgca tgccagattt tttctccttt attaacagcg tcctttgggg atcggtaatg
    12481 atttacctgc tcttcggcgc aggttgttgg ttcacttttc gcaccggatt tgtgcagttt
    12541 cgctacatcc gccagtttgg caaaagtctt aaaaatagca ttcatccaca gccaggcggt
    12601 ttaacctcat ttcagtcatt gtgtaccagt cttgcggcgc gcgtgggtag cggcaacctg
    12661 gccggcgttg cgctggcaat taccgccggt ggacctggag ccgtcttttg gatgtgggtt
    12721 gccgcgttta tcggcatggc gacctcgttt gccgaatgtt ctctggcaca actttataaa
    12781 gaacgtgacg tcaatggaca gtttcgtggc ggaccggcat ggtatatggc gcgcgggctg
    12841 gggatgcgtt ggatgggcgt tctgttcgcc gtctttttgc tcatcgccta tggcataatt
    12901 ttcagcggag ttcaggcgaa cgccgttgcc cgcgccctaa gtttttcttt tgattttcct
    12961 ccgctggtga caggcattat tctcgctgtc tttgctctgc tggcaatcac tcgcggtctt
    13021 catggcgtcg cccggctcat gcaggggttt gtcccgttga tggcgataat ctgggtgctg
    13081 accagcatgg tgatttgcgt aataaatatc gggcaacttc cccacgtcat ttggtctatt
    13141 tttgagagtg cttttggctg gcaggaagcg gcaggcggcg cggcgggata taccttaagc
    13201 caggcgatta ctaacggttt tcagcgcagt atgttttcca atgaggcggg aatgggttcg
    13261 acgccaaacg cggcagcggc agcggcgtcc tggcctccgc atccggcagc gcaagggatt
    13321 gtccagatga ttggcatttt tatcgacacc ctggtcatct gtacggcaag cgccatgctg
    13381 atattactgg cgggtaacga cacaacctac atgccgctgg aaggtattca gcttatccag
    13441 aaggcgatgc gggtgctaat gggttcctgg ggtgctgagt ttgttaccct cgtggttatt
    13501 ctgtttgcct tcagctccat cgttgccaac tacatttatg ccgaaaacaa tctcttcttt
    13561 ttacgcctga acaaccctaa agcgatctgg tgtttgcgga tctgcacctt cgcaacggtc
    13621 atcggcggca ccttgctaag ccttccgctg atgtggcaac tggcagatat cataatggcc
    13681 tgcatggcta ttaccaattt gaccgccatt ttactgctct cgcctgtggt tcataccatt
    13741 gccagtgatt atctacgcca gcgtaaactc ggcgtgcgcc cggtgtttga tccgttgcgt
    13801 tatccggata tcggtcgcca gctttctccg gacgcgtggg atgatgtttc gcaggagtaa
    13861 tcacaactat cgatcaactc attctctttt tttgctaaag tcggcataaa tttcctgcaa
    13921 ggactggata tgctgattct tatttcacct gcgaaaacgc ttgattacca aagcccgttg
    13981 accaccacgc gctatacgct gccggagctg ttagacaatt cccagcagtt gatccatgaa
    14041 gcgcggaaac tgacgcctcc gcagattagc acgctgatgc gcatcagcga caaactggcg
    14101 ggtatcaacg ccgctcgctt tcatgactgg cagccagatt tcacgccgga gaatgcccgc
    14161 caggcgattc tggcgtttaa aggtgatgtc tacaccggct tgcaggccga aaccttcagc
    14221 gaagacgatt tcgattttgc ccaacagcat ttgcgaatgc tttccggctt gtatggcgta
    14281 ctgcgcccgc tcgatttaat gcagccttat cgtctggaaa tggggatccg tcttgagaat
    14341 gcccgaggga aagatctgta tcaattctgg ggagatatca tcaccaacaa gctgaacgag
    14401 gcgctcgcag cacaaggcga taatgtggtg attaatctgg cgtcagatga gtattttaaa
    14461 tcggtgaagc cgaagaaatt gaatgccgag attatcaagc cggtgttcct cgatgagaag
    14521 aacggcaaat ttaagataat cagcttctac gctaagaaag cacgcggtct gatgagtcgt
    14581 tttattattg aaaatcggct gaccaaacca gagcaactga ctggttttaa tagcgaaggt
    14641 tacttctttg atgaagcttc ctccagcaat ggcgaactgg tgtttaaacg ctacgagcag
    14701 cgttaagttt gtagtcaatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca
    14761 tgcagattca atatattgca gaa
//
LOCUS       DAEVZA010000121        14733 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEVZA010000121 DAEVZA010000000
VERSION     DAEVZA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.121"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            382..558
                     /locus_tag="LAF92_003834"
     CDS             382..558
                     /locus_tag="LAF92_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792766.1"
                     /translation="MNLVDAFVKKVISEPYEEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(623..919)
                     /locus_tag="LAF92_003835"
     CDS             complement(623..919)
                     /locus_tag="LAF92_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792767.1"
                     /translation="MSALRHVLSDILFSGKNRLQVLAKTSGGAGFGALVGEELSITDT
                     IETVVTGSVRANNGVLSPVMEKRMINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            1219..1470
                     /locus_tag="LAF92_003836"
     CDS             1219..1470
                     /locus_tag="LAF92_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792768.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(1542..1694)
                     /locus_tag="LAF92_003837"
     CDS             complement(1542..1694)
                     /locus_tag="LAF92_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBI9792769.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            2009..3100
                     /locus_tag="LAF92_003838"
     CDS             2009..3100
                     /locus_tag="LAF92_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein finQ"
                     /protein_id="HBI9792770.1"
                     /translation="MEMSQLKQPIFLKKIKKVINTIPGLEEQIFACRNKKRSDNPLLF
                     IDRKDEERILMSRLQSQQKNEELASKLESLFHGNELSSPHSILCFIYWRYTKKIYRLS
                     EDIISDVANTYVDNIPAQILKELPSWSIYVSAENLHTILPTSYPIHGFFFYPFLDNNG
                     NIIRLFIIDDLKQSQGTTGLKEKDVDVVNNIIRIKDSREGLLDSRKMECIDGEVVVTV
                     NEKLKDFRDREFNLLNAQISMVLYICSQINDIKEKNQFKRSEKHKKHVHTHHELPAQN
                     IREWDVGIRMGQAIRQYRQAEPTGKERTTIGSKRPHIRRGHWHTYWTGSKKPELAHER
                     KPRLIWLPPVPVNLEDVNKLPVVITPIDK"
     gene            3407..4615
                     /gene="trbA"
                     /locus_tag="LAF92_003839"
     CDS             3407..4615
                     /gene="trbA"
                     /locus_tag="LAF92_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HBI9792771.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYTSCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPKKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            4634..5704
                     /gene="trbB"
                     /locus_tag="LAF92_003840"
     CDS             4634..5704
                     /gene="trbB"
                     /locus_tag="LAF92_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HBI9792772.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            5697..7988
                     /locus_tag="LAF92_003841"
     CDS             5697..7988
                     /locus_tag="LAF92_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HBI9792773.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     LTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(8025..10724)
                     /gene="nikB"
                     /locus_tag="LAF92_003842"
     CDS             complement(8025..10724)
                     /gene="nikB"
                     /locus_tag="LAF92_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HBI9792774.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(10735..11067)
                     /gene="nikA"
                     /locus_tag="LAF92_003843"
     CDS             complement(10735..11067)
                     /gene="nikA"
                     /locus_tag="LAF92_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HBI9792775.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            11301..11636
                     /locus_tag="LAF92_003844"
     CDS             11301..11636
                     /locus_tag="LAF92_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBI9792776.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(11722..12570)
                     /locus_tag="LAF92_003845"
     CDS             complement(11722..12570)
                     /locus_tag="LAF92_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBI9792777.1"
                     /translation="MNQTLPTADLNTAGTADVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEKGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            complement(12695..12976)
                     /locus_tag="LAF92_003846"
     CDS             complement(12695..12976)
                     /locus_tag="LAF92_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792778.1"
                     /translation="MPLLCRRFSPVAPDAALPAHLTVCKAIAPDGAAISACLPPHCGL
                     FSADGLSGGTYTTTSRFSRPGERCTSHKTFCRWRQKLLRLPPDNCPVTW"
     gene            12919..13116
                     /locus_tag="LAF92_003847"
     CDS             12919..13116
                     /locus_tag="LAF92_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792779.1"
                     /translation="MQGAPHPARPEKTDGTTTACGGHWYTTPAQVKTEKTARHPQSKK
                     VKFLHFFTLIFRADLVIMVIK"
     gene            13150..13401
                     /locus_tag="LAF92_003848"
     CDS             13150..13401
                     /locus_tag="LAF92_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9792780.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMHKADIIRRLENELGETAEDV"
     gene            complement(13432..13677)
                     /locus_tag="LAF92_003849"
     CDS             complement(13432..13677)
                     /locus_tag="LAF92_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792781.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(13677..14591)
                     /locus_tag="LAF92_003850"
     CDS             complement(13677..14591)
                     /locus_tag="LAF92_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBI9792782.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGALEKALAIAC
                     QLQKMGMTPEQIKQATGLSDDELKKITH"
BASE COUNT         3911 a         3768 c         3456 g         3598 t
ORIGIN      
        1 cttcatgaaa taaacaatgg cactcacttg caaacaggag tccactatcg caagcgactc
       61 aacttagcaa tatctctcaa tttgaatgaa aaaaccagat ggcatgtgac agggaaccgt
      121 ccgcatgatg tccgtgacca tttaacccca taagctaacc ttgtcacaaa cgggcgcaga
      181 acagcctgcc agacagacaa caaaacaaaa atccaagata aaacatgaat accggtatct
      241 caatgcgaag aaataatctc cgtagaaacc cccatgagaa aaatatcata tgggagaaaa
      301 tcgcaattat cacagccagc gcattcatta ttttcgctgt gctctggcat taattatttt
      361 caacacaaaa aggaaaaaac aatgaatctg gtagacgcct ttgtcaaaaa agtaatatcc
      421 gagccatatg aagaatatgg aaaatggtgg atagatgttg aatacatttc atggggtgtt
      481 cccgggaaaa caagactgat gtttgagtca aaggagcagg cactggaagt taaagaaggg
      541 tacaaatttt taacgtgaga tctctttttg ttaaagggca ggaagcggta actccctgcc
      601 cttagttacg tttccaggct tatcagtcga taagttcccg gatcagttcc agaacagcaa
      661 tcagcgcgtt tgcaacggca actactgccc tgagaacgat gattaacaga ttaatcatcc
      721 gcttctccat cactggagaa agaaccccgt tgttcgctct tacactgcct gtcacaacgg
      781 tttctatagt gtcagttata gacagttctt ctccgaccag agcaccaaaa ccagcaccac
      841 ctgatgtttt ggccaggact tgcaacctgt tcttcccgct gaacaatatg tcggacagaa
      901 catgcctcag agctgacatg ccgagtatac acttatccag tacttgcaga aatataattc
      961 cagacgtact atctacaagt cagttataga cagttctttt ccgaccagag ccacccactc
     1021 tgtaacggaa cagtaaaaaa accgacctca ccagtcggtt tttttacatc tgttgtttct
     1081 caaaatccgc ccgacatgcc cgtcacaccg gggaaactca tgctttgaat cagaaccaat
     1141 catctcaact ttgccgtctc ctgaaaaaac tggcacattc agttcgataa ctacccataa
     1201 atacagagag aaaatccgat gtcagaaaag tggagtaaag aattcgactc ttgctttcag
     1261 gtggggagca tagaagaaat tgccgatgct ctcgagaaac gcagcggaag cgaggaaaac
     1321 gcgttagcca tgctggcaat gacagccttc acactcatga cccgacgtgg tatctgcgaa
     1381 atgcagaacg tggcccccga tggcaaaggc attcggctgg agctaatcgg cttcagggaa
     1441 aatgaaacaa taccagatac gcttcattga agaagaaagg gcgcaatgag gtcagccttc
     1501 gcaacaaagc ggcggcagaa aacctgccgc cgtgttgccc gttaacgttt aacttcgtag
     1561 gctaacgttg ccacaagcac tgtgtttccc tgctggagcc ggagtccgca aagcgaatcc
     1621 ctcaccatcc agacaaaaca cagaatcgtc acacagacga cgattaacat cattaaaaac
     1681 gttcgctgtg gcatcatagc ccctccattg ccttccggcg gttatcgggc tactataatg
     1741 ttgttgaggc attacagtag agcccttggt tgattgataa aaaaatctcc aggggctttc
     1801 tgctgtatac cgtccacaaa acgctttcgg catacagcct caagcgcccg cagctattct
     1861 aacgactttc gtatctgtaa caatcctttt attatttgaa tgattcagcg ttgtttttga
     1921 agttaacaac caacccaata catggttctc tacttcacaa caacgcatgt tatgctgatt
     1981 ttagttaata aaagaatccc cttaggagat ggaaatgtcg cagttaaaac aacctatttt
     2041 cctgaaaaag ataaaaaaag tcatcaacac aatccctggg cttgaagagc aaatctttgc
     2101 atgccgaaat aaaaaacgca gcgacaatcc attattattt atcgacagaa aagatgaaga
     2161 acgcatacta atgagtcgtc tccagagcca acaaaaaaat gaagaactgg cttctaaact
     2221 agaatcttta tttcatggaa acgaattatc gtcaccacac agtattttgt gttttatata
     2281 ctggcgctat acaaagaaaa tttatcgcct aagcgaggac atcatcagtg atgtggctaa
     2341 cacctatgtt gataacattc ctgcacagat cctcaaggaa ctcccttcat ggagtatcta
     2401 tgtttcagcc gaaaatctgc atactatact tcctacgtcc tatccgatac atggattttt
     2461 cttttatcca tttttggaca ataatggaaa tattatccgg cttttcatta ttgatgatct
     2521 gaaacaaagt caaggaacta cagggctaaa agaaaaggat gttgacgtcg tcaataatat
     2581 catcagaata aaggactctc gtgaaggact tcttgactcc agaaagatgg agtgtatcga
     2641 tggtgaagtc gtcgtaacag ttaacgaaaa gctcaaagat ttcagagaca gggagtttaa
     2701 tcttcttaac gcacagatat caatggtgct ttatatctgt tcacagatca atgatataaa
     2761 agaaaaaaat caatttaaac ggtcagaaaa gcacaaaaag catgttcaca ctcaccatga
     2821 actaccagca caaaacattc gggagtggga cgttggtatt cgaatggggc aagccatacg
     2881 gcaatatcgc caagcagaac ccacagggaa agagcggaca acaataggta gcaaacgtcc
     2941 tcatatacgc cggggacact ggcatacata ctggacagga agtaagaaac cagaactagc
     3001 ccatgagcgt aaacctagat taatatggtt acctccggta ccagttaatt tagaagacgt
     3061 aaacaaacta ccggtggtaa taacacccat tgataaatga cctgaaaagt atcttttccg
     3121 gtaggatgta cacatatcta aattatcagg ctactataat gttgtctagg cattacagta
     3181 gagcccctgg ttgattgata aaaaatctcc agggggcttt ctgctgtata ccatccacaa
     3241 aacgctttcg gcatacagcc tcaagcgtcc gcggctattc taacgacttt cgtatctgca
     3301 acaatccttt tatcacacgg ctaattcctc tggttagctc ttatcgcctg caaataaatt
     3361 tttaaccttt ccgtttgatt taaattccag aaatggagac tttgtcatgt cttacaatcg
     3421 acagcctgtc gcagaagatc ctatgcagat atggggcgct gttggtgtcc ttcttatcct
     3481 gctgctattc gtcatatggt tattcttgcc ggaagtggtt tacaccagct gcctgattct
     3541 tcatacctta tggggactgg tcgactgggg accatttcat aactatgcag caccacgata
     3601 caacctgctt gccatgaccg gtaacaacgc cgccaacata agctatagcc agtgggttaa
     3661 cgtcatggaa cagaccatag gtattctgtg gatgtacctc ttacctgtga cactctggtg
     3721 tttgtgggaa tggtatcaac atccgggaca aagccgtttc acccgcagac cagtagatat
     3781 tacccgactg ccacacatct ttgcttcgtt atcccctgct atcgcccctg tactggcaga
     3841 tggtgatccg aaaaaactct ttcatggtgg taaacgacct gaacggcggg ttgctctcac
     3901 cccggaagca tttgtcgaac agcatactct catcaccaat atgcagctcg atgttgcagc
     3961 tgcaagacga tgtttcatgg cccagttggg taaaccttta acgtcctgga aagacatggc
     4021 accacatgaa aaagcgctct ttgccatatt tggccttcag tatttcctgg atgatcggaa
     4081 ggcagcatta aagctcatgg atacactgaa cctctcatgc agaattaaaa gcaaacggga
     4141 cagcgggaaa ttctgtaccc ctgtttattc actggccaaa tcagctttcc agagagtgat
     4201 taaaagcaac ggagcacagc aatggctgaa acaacatcgc tacgtccgca gtgggctggt
     4261 ctggctgtat gcacatgacc tgcggcttac gccacccaac tggatttggc tgaaaggcgt
     4321 ggacagaacg ctgttttatg cattacaccg cgccaatacc acgaaaggat ttattgaagg
     4381 cgctggcgtg gtggccgttg cacgtgcaga ggccgaagcc atgcgttttg gtcttccctg
     4441 ccctgaaccc tgtgttgatg aagcggtgga aggcttacgg cgggatatgc tcagtctcgg
     4501 tcttatctgg gatgaaccac aaccagatcg cgaccggaaa cgccgcatcc tgaccaactg
     4561 gtccctcacc gacgacatcc tgccccgcac acctgctaca gacaacgaat tctgattatc
     4621 cggagaactg aaaatgacaa tcgagtactt tgcaacacgc attgcaaaac atatttccgt
     4681 gcaggcaatc tggccggatg gcaggacaga aattattgct gtccttccac gggatgccct
     4741 gtcagggctg gtaacacgaa ataatcaact gattcatata gcctttgacg ccatgggcac
     4801 ccgtaacaga gaaaccgtcc tgattgataa acaggagcat aacgctgacc agatacttac
     4861 agctattgat tcctgcctgc gccttgaata tctgatggaa cgccgcttca gcagaacacc
     4921 tttgttccgt ggcataatcg ccagcgttgt gctgttcgta atggcaacga ttggcttctc
     4981 tctgttccgc tatgttgatc gtgtattctg ggatgataca acgcctgaag cagtacagac
     5041 agcaggtgag ccacgactgc taccccctca cctgaatcac actgtgccat taaatgaagg
     5101 cattcaactc ccggttcccc cgaaaaaaga cgtccaggtc ccggagaaaa caatcacaag
     5161 caaaaatccg gaagccgcag ctgcgcgaca taatctggct actgttctga agcggaacgc
     5221 cgatcggggc atgtttacca tcaacctgtc atccggtcat gaacgaaccc tgttcgcatt
     5281 tctcgatcct gcctgtccta actgcagact tctggagccg gccttaaaac gcctggccag
     5341 cgacttcaat gtcgttatct acccggtttc agttattggt ggagaggaat ccaccgaccg
     5401 tgtggcacca ctgctgtgtg aaaaagatgc tcagaaacgt gctgccggct ggcaccgact
     5461 gtattccgca gataacggaa tgatgacgcc atcagaagaa acaacgcctg cagatgaaac
     5521 atgcctgaaa gcagccagag ctgcgattga tgttaacaat gtcgccttcc gtaagttcgg
     5581 atttgccggt accccatggg tgctgtccga caccggctgg catctgccta caggaattct
     5641 gcaggaaact ggcacgctta atttattcct gaaaaccacc gacagcgagt ctggccatga
     5701 gtgaacatcg cgttaacccc gagcttttac accgtacagc ctggggaaat cctgtctgga
     5761 atgcactcca gagtctcaat atctatggct tttgcctggt ggcaagtctt gtggccagtt
     5821 ttatctggcc acttgcatta ccggcatgtc tgttgttcac actgataaca atgctggtat
     5881 ttagtctgca gcgatggcgc tgccctcttc gtatgcccat gacgctggaa tgtgctgacc
     5941 cgtcacagga cagaatgatt aaaagaagtc ttttcagctt ctggccaacc ctgttccagt
     6001 atgaggtcat tctggaatca ccggccagcg gcatattcta tgttggttat caacgcgtca
     6061 gggatattgg cagggagtta tggctgagta tggacgatct gacccgccat ataatgtttt
     6121 tcgccaccac cggtggcggt aaaacggaaa ccatctttgc ctgggcgatt aacccacttt
     6181 gctgggcaag aggattcacc cttgtcgatg gcaaggcaca gaatgatacg gcaagaacga
     6241 tctggtatct ggccagacgc tttggacgtg aagacgatgt ggaagtcatt aacttcatga
     6301 acggaggaaa atcccgcagt gaaatcattc tgagtggtga aaaaacgcgt cctcagtcaa
     6361 atacctggaa tcccttctgt tacagcactg aggcattcac agcggagaca atgcagtcaa
     6421 tgctgcccca gaacgtccag gggggcgagt ggcagtcccg ggccattgcc atgaataagg
     6481 cactggtatt tggtactaaa ttctggtgtg tgcgcgaagg caaaacaatg tcgctgcaga
     6541 tgctccgtga acatatgact ctcgaaggga tggccaaact ttactgtcgt ggcctcgatg
     6601 atcagtggcc ggaagaagct atcgcaccgt tacgtaacta ccttcaggac gttcccggtt
     6661 tcgacctgtc gctggtcagg acaccatcag cctggacgga agaacccaga aaacaacatg
     6721 cttatctctc cgggcagttt tctgaaactt tcagcacatt cacggaagca ttcggggata
     6781 tttttgctga agattccggt gatatcgata tccgtgacag cattcacagc gacaggattc
     6841 tgatggtaat gatccccgct ctggatacat cagcgcatac aacgtctgct cttggacgaa
     6901 tgtttatcac ccagaaaagt atgattctcg cccgtgattt gggctaccgc ctcgaaggaa
     6961 ctgattccga tgcactggaa gtcaaaaaat ataaggggcg tttcccttat ctgtgtttcc
     7021 ttgatgaagt gggggcatac tatacggacc gtattgctgt ggaagccact caggtgcgtt
     7081 cactggattt tgctctgatt ttgatggcac aggaccagga acgtatagaa ggacagacaa
     7141 cagccacgaa tactgcaaca ctgatgcaga acacggggac aaaatttgcc ggcaggattg
     7201 tcagcgaggg tagtacggcc agaacgctga aaagcgccgc cggtgaagaa gccagggcga
     7261 ggatgaacaa cctgcagcgt caggacggca tatttggtga atcatggatt gacagcccac
     7321 aaatcagcat cctgatggaa agcaagataa atgttcagga gttgattgaa cttcatcctg
     7381 gcgagttctt ttctattttc cggggagaaa ccgtaccatc agcatcattc ttcatccctg
     7441 acgatgagaa aagctgcagc agcgatcccg ttgttataaa caggtatatc agcgtggatg
     7501 cgccacgcct ggaccgtctc cgccgtcttg ttccccgtac tactcaacgt cgaataccgt
     7561 caccggaaaa tgtcagtgcc atcattggcg tcctgactgc aaaaccctcc agaaaaagac
     7621 gaaaaattcg cacagagccg cacacgatcg tggatacatt ccagcaacgc attgcaggac
     7681 gtcaggcggc tatggcaatg ctggaagagt acgacacaga tatcaacgca cgtgaaagtg
     7741 ccttgtggga aacagcggtg aatacgctta aaacgaccac acgggaagag cgacgaattc
     7801 gctatatcac gctgaacaga ccagaactac ctgaaacaaa agaggaaaat cagatatcag
     7861 tgagagcaga aagagcaggt ataaacctgt taacgcttcc acaggacaat aaccacctga
     7921 cagggcgtcc tgtaaacggg tttcatcata aaaaaaccaa ccgtccggac tgggacggaa
     7981 tgtactgaac agtggccgcg ccggattctg gcgcggcctt ttttttaacc tggagaaggc
     8041 ggttcccagc gtttatcctg ctccggcgga ggcagtgatg cgtcatcgtt gctgttcgcc
     8101 gcgatataat cgcggtagta gcgttcactt ccttcacggt gatgatccac gtccggtcgc
     8161 gtaatcctgt agtcttcgtg accggtacgg cctgtcacat tatgccaggc aaccatttca
     8221 gcaaagacct ggttaaattg tttgtcgttt ccttctggtt caaaacccat gcgtggatca
     8281 cgcccgaata ataccggtgc aatcaaatcc agtttatctg ccagcgcatt gcggtcatgg
     8341 tgattacgga aaacaaccct gtcaccataa tccgtacaga caaactctcc cgtccgacgg
     8401 tcacggaaac gaacactccc gttcttctgc aaacgggcct cgagatcacc ggtattaccg
     8461 tataccggcg ttccgcccgg ttcacaaagc acgacacagc ggtctgttgt cgttgtgcgt
     8521 gacttatctt tacggcgatc gcgataatcc cagcctctca gctgcgatac agcagccctg
     8581 tctccctgag cagcctgaat ttcgacccac tgacggtaag agggtggata ccacttcccg
     8641 tcagcaataa gtttctgccg ctcatccctg atgtcttctt tcagcctgat caatgcctgc
     8701 attcgctgaa cttctgcaat gtgataatgc agcttacgta atgccggatc atcgtactgc
     8761 gcacgaatgt gtgacttccg cagacggcat gcctgatgaa tttcccgaca acgttcccca
     8821 taacgcagat ctggtttacg ccattgttca cgccatgcca gatagcgggc acgcaggtca
     8881 gcacgggcct cagctctggc ttcacgtcgc tgccggcgca gcattgggtc acgtttactg
     8941 ctgaccccgt acctgtcact gacagccagc tccgggttat agcgactttc aggttgcacc
     9001 ctgtcaaaca aatctgctgg ggcactcaca aaaggtccgg cctgtggctc tgcacgcccc
     9061 agagtcagat caggatgaat gctgctggcc ttcaccggcg tttgctcatg attaaaggca
     9121 tcaacgacaa caagcccgtg atgttgtttc tgtagcatca gtccctgttg tgcaaacaac
     9181 tcatgacaac gctgccaggt aaactcaccc tgtgtgatca tttcttcaat ctgagggcta
     9241 caatggctga tgctgtatcc ccacagactt tctttgtcag aaaacagttc gcgtttgata
     9301 gccccctgcg gccccgtacc tgtacgcata cggtgccatt ctttatcggt cgcaggacag
     9361 ctctccatac gacgaccacg gaagcgttct gttggcgtaa cctggcggaa aatatctgtt
     9421 ggtacaggct gccagccacc atcgagttga ttcacattgt cgagcgtcag cagtgaccat
     9481 acgctgtcag cacgaacggg agtctggtta tgatcataac cgtcacagat aaccaggtga
     9541 ccatgctgaa cacgcatcca taatcccgct tcagccagtg tgcggtgaat agcctggcag
     9601 ttttccagcc gtccttcccg tgccatttcc ggcagacgtt ctccaagatg acgacaggca
     9661 tactgctgca gactttctgt ttcagcgatt ttttccgggc gaacatcagc tgcaatgaag
     9721 tccggattat agcgtcctgg tgcttccacc tggctgaaaa tgtcttttgg cactggcgtg
     9781 tagtcaccca tttttttcat gagtttttct gcacaccagg agggaccaaa cgagtcaagc
     9841 tgtacacctt ccctgttgcg atcccagcca tccatcacca gaaattttcc gtccatctga
     9901 gacaggtaca aaccatcttc agcaagacga cgatgcaggg ataaccagtc atgtacaggt
     9961 tcactgcgta aaccagcgac cgctgtctgc gcaacatact cgcgaaaagt ttgttttttt
    10021 ccgcgtgtcc aagcgttctg gcgatcacgt tcaacggcag ttttgcgaac gatacgattt
    10081 cccggcgcat ggacccagca accgttatcc ggagcaaaac catgcttcag ttcaagctca
    10141 cggcaggcac gactgagttt ttcctgactc catgataacc ggttcagata acccgtttca
    10201 gggtgtaccc ggttaacagc cacatggacg tgcagattat ctgtatcagt atgcacggca
    10261 gagacatact gatggtcggc aaggccgagt gatttcagcg tatgacgtac actgtcataa
    10321 atctgttccg gacgcgggct ttcatgtgac tgccaggaaa ggatgtagtg aaaaacagga
    10381 tcagtgtcat cttttgcata gtgtgcctgc cgggcaatgt actccatatc tgcagcggca
    10441 gtttcaagag aagtacagtt atgaaagcag gtgacaccat aaaagttgac ccattcgcag
    10501 ccgtccttca tgacatcaac cagcgccacg aatgactcat ttcgaagacg tgtcgcataa
    10561 tcaacaaggc gactgaaacg gcttcggtgc ggttgttcag cctgcgaaga agaagataaa
    10621 tccagttcct catcggtcat gtcatcccgg acagagacgt aggataccag gtcttcgaac
    10681 gaagacttgc cgtcccttct ctttttcgga atgactgcat tcaccggaga atcctcaacc
    10741 ttccattaac tgtttacgca atgtatttgt aagttcggtt atggcaacaa gcacttctgc
    10801 gtattccttg tcaccggttc tttttccctg aacaaacaga tgcttctgca tccttcccag
    10861 tctcattaac tccttcagaa aagcatcatc tgtacgggag ttaattcgct tattcagtgc
    10921 tgcatttcgg atgtaagcag atacagtaag tccggaatct tcagcttttt tcctgatggt
    10981 ttcatcctca acaggagaga atctgagtgt cctgacaact gtcttttgcc gtacctcact
    11041 tttctttctt accgcactat cactcacttc aggctcctta cggggtgtcg gggcgaagcc
    11101 ctgaccagat ggcaattgta atagcgtcgc gtgtgacggt attacaattg cacatcctgt
    11161 cccgtttttc aggccattat agcctgttat ttttgtgcat catagtctta tgcagacggc
    11221 agaggcaggg cgcagccctg ccaggagtca gtttttgatg ataaaatcag gcttcaacat
    11281 gaaacggaga gaaagtgcca atggcttcta aaaaatttta ctcagatgat gacatccagc
    11341 tggcaaaagc tgctttatcg gaattgccag atctcaccgc tcaacggaaa acattgcgtg
    11401 attttcttga tgccattcgt gatgacatta ttgtcctggt aaggacaaag ggttatacac
    11461 tggcagatgt tcgtgacacg ctcaaaaatg cagggtatga agtaggagag aaggcacttc
    11521 gtgacattat tcgggaagca gaatcgaaaa aaccatcccg ccgttcttcc tctaaaacag
    11581 cacctaaaaa gacagattca ggcaggaaag atggcatcga tatgaacaac aattgatgtg
    11641 acaatgtcac attttgttct gagattatga tgattacaga aaaaaaagcc ccggcataaa
    11701 taccagggct tttctgttta atcagactcg tggcggcaat acacctggat aatgccgtgc
    11761 aataatatca ttcagttttt caacaaggtc agaacgctta aagacaatgt gtacggtccc
    11821 tttctggtag ccacgcaccg taaaatattc cccttctaac agcttaccga cattgtcgcc
    11881 gttcccgaga aattcaccat actgcgcccc gatgctgttc ctgaagtcag gaacgttgcg
    11941 cccctccagc agataaaacg gacgtaccag gtcatccagt gcctcccgtc cgctgcagtc
    12001 cagcgtcaca cgcccgttac tccagcggta cagcaggttt tcaataatga ttttcttacc
    12061 cagacggcag ggattattgg ttctgtaatc ccatgacaat ttccggtaga catcaatcag
    12121 tcccttttca aatgtctgca tcttactggc attcagatgg cggaatgttg ccagtacatt
    12181 gtccagggtg atttcaggac aggtgtcgct catcagctgg ctgttccact catcacgctg
    12241 gcatgaactc atgaaggtgt acatgcccgt atctgtcatc aggcggtccc agattttccg
    12301 gtcggcaaga cgggtgatat tccttttcgc ttcttccggc ttatccatgc gacgaacgga
    12361 atcagtcacg cagtcttcaa acccgtaaag aaaaatatca cctgacgcat cgagcagaat
    12421 cttgcgcgct gtcgccaggc attccatcgc ctgcatgaat agtgcaatac cttcgttacg
    12481 ctgcgcgatg atgcggtcga tagccacaga cggaataaca tctgccgtgc cggcagtatt
    12541 caggtcagca gtgggtaaag tctgattcat cacattgtct cctcatcgtc acgactgccg
    12601 ggtcagccag gtcatttccc ggaggagcga agctccttct gccgtccggg tcttagggta
    12661 tttccccgca gtggaccttt ccgacggggg acgctcacca cgtcacgggg cagttgtccg
    12721 ggggcagacg caaaagtttt tgacgccagc ggcaaaaagt tttgtggctg gtacatctct
    12781 cccctggacg actgaaacgg gatgtggtcg tgtatgtacc cccggaaagt ccgtctgcgg
    12841 agaacaggcc gcagtgcggc ggcagacagg cagagatagc agcgccatca ggcgcaatgg
    12901 ccttacacac cgtcaggtgt gcagggagcg ccgcatccgg cgcgaccgga gaaaaccgac
    12961 ggcacaacaa cggcatgcgg tggtcactgg tacaccactc ccgcacaggt gaaaacagag
    13021 aaaacagcac gacatccaca atcaaaaaaa gtgaaatttc ttcacttttt cacacttatt
    13081 tttcgcgctg atttggtgat tatggtgata aaataaagtg aagttttttc actttttcaa
    13141 aggtatttta tgaccggctg ggaactgcga atctggcgaa aaagcatgct ctggtcacgc
    13201 gaaaaagcgg ccagagaatt tggcgtgaca cagagaacat ggcacgcctg ggaaaatgcg
    13261 gagcaggtgg atgtcaccgt ctggaggacc acccaggcac tttcagtccg tgacctgctg
    13321 cctcacatgc aggggatgca caaggcagat atcatcaggc gactggaaaa tgaactgggg
    13381 gaaacggcag aggatgtgtg agcgtaattc acagcctggc tgaatgccag gctactgctc
    13441 atcaggaaac agttttttca gttcttcacc ggaaagtccc gtgacctcct gaatccgttc
    13501 cggcggccag cctatatccc gaagccggca ggctatttcc cgcgccactt caagagcccc
    13561 ttgtttaaaa ccctcgtcga aacctttctg tataagacgt tctgcaatcg tcatgctgtt
    13621 tgctctgctc ctgctgacaa aaaagcccgg cattttgccg ggctttgtgg ttctcatcag
    13681 tgagtgattt tcttcagttc gtcatcggaa agtccggtag cctgcttaat ctgctccggc
    13741 gtcatcccca ttttctgaag ctggcacgca atagccaggg ctttttcaag cgctccttcc
    13801 atgcgtcctt ccatcctgcc ttcctggcga cctttttgct caagacgttc agcgatagtc
    13861 atcagtttat cctcatgctg cggcagacgc tcagccagcg cccgcataaa ttccggtgat
    13921 acagagtccc ctgcgttaac gatgtaatga atcagaacac gcacctgctc atctgaaatt
    13981 tccaccatga cctgcgggag tttgtccagc agctccatca tgtcacgatg gcggatatgc
    14041 ttcatcagca gcgtcagtgc ggccatccgg cgatggttca tgatttcatt gtcatcaacg
    14101 acagtgatat caaccagcgg aaacggcttt gtgtatattt tagccgcaag tgccggattc
    14161 tcaaaacagt ccagccagtt catgctgtac gggtacgggc tgcgctcacc atgatagaac
    14221 agtacaggaa tcaccagtgg caacgttttg tgctgctcca gatgtctctg catcgccgcc
    14281 acactgtaac gtaacatgcg gaaaggcatg tacagatttt cggtgctctg gtgctcgatg
    14341 agcgtataaa catagccatc ttcacccccg gtggttttca cgctccagag gatatcgctg
    14401 gcgtactgat gcaggtccgg ctcaacaaag gtggcgggtt ccagcttcag cgtggacagg
    14461 tcgcacaact gccggtactc cgccggaaga tgcagttcca gaaaatctct ggcgacgtcg
    14521 ggattcgcca ggaacgaccg gaacgcggca tcatgcggcg tgggcgttgt ggtgtttttc
    14581 ttcttactca cggttcctcc tggtgagatg tctgaacgta tcctgccgcc tgcacaggca
    14641 gacggcaagg gttaatcagt ggcagacccg ctcaatttca agcgcgcgga gcaggtggcc
    14701 ccattcgctg actttcatgg cggggatacg cac
//
LOCUS       DAEVZA010000122        14666 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEVZA010000122 DAEVZA010000000
VERSION     DAEVZA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.122"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..815
                     /gene="selA"
                     /locus_tag="LAF92_003851"
     CDS             <1..815
                     /gene="selA"
                     /locus_tag="LAF92_003851"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBI9792783.1"
                     /translation="GCTLHEVGTTNRTHANDYRQAVNENTALLMKVHTSNYSIQGFTK
                     AIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEPMPQELIAAGVSLVSFSGDK
                     LLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAALEATLRLYLHPEALSEKLPT
                     LRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLSQIGSGSLPVDRLPSAALTF
                     TPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRCLEDEQRFLEMLLK"
     gene            812..2656
                     /gene="selB"
                     /locus_tag="LAF92_003852"
     CDS             812..2656
                     /gene="selB"
                     /locus_tag="LAF92_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBI9792784.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            2846..3997
                     /gene="yiaY"
                     /locus_tag="LAF92_003853"
     CDS             2846..3997
                     /gene="yiaY"
                     /locus_tag="LAF92_003853"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBI9792785.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLFKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            4162..5700
                     /gene="aldB"
                     /locus_tag="LAF92_003854"
     CDS             4162..5700
                     /gene="aldB"
                     /locus_tag="LAF92_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBI9792786.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(5919..6062)
                     /locus_tag="LAF92_003855"
     CDS             complement(5919..6062)
                     /locus_tag="LAF92_003855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792787.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            6245..6568
                     /locus_tag="LAF92_003856"
     CDS             6245..6568
                     /locus_tag="LAF92_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBI9792788.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            6574..7710
                     /locus_tag="LAF92_003857"
     CDS             6574..7710
                     /locus_tag="LAF92_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI9792789.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFIHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(7707..8681)
                     /locus_tag="LAF92_003858"
     CDS             complement(7707..8681)
                     /locus_tag="LAF92_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9792790.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            8805..9545
                     /locus_tag="LAF92_003859"
     CDS             8805..9545
                     /locus_tag="LAF92_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HBI9792791.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(9567..9755)
                     /locus_tag="LAF92_003860"
                     /pseudo
     CDS             complement(9567..9755)
                     /locus_tag="LAF92_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9759..9860
                     /locus_tag="LAF92_003861"
                     /pseudo
     CDS             9759..9860
                     /locus_tag="LAF92_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(9892..10587)
                     /gene="araD"
                     /locus_tag="LAF92_003862"
     CDS             complement(9892..10587)
                     /gene="araD"
                     /locus_tag="LAF92_003862"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBI9792792.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(10581..11441)
                     /locus_tag="LAF92_003863"
     CDS             complement(10581..11441)
                     /locus_tag="LAF92_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBI9792793.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(11434..12096)
                     /gene="ulaD"
                     /locus_tag="LAF92_003864"
     CDS             complement(11434..12096)
                     /gene="ulaD"
                     /locus_tag="LAF92_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBI9792794.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(12093..13589)
                     /gene="lyxK"
                     /locus_tag="LAF92_003865"
     CDS             complement(12093..13589)
                     /gene="lyxK"
                     /locus_tag="LAF92_003865"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HBI9792795.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSDVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(13593..14579)
                     /gene="yiaO"
                     /locus_tag="LAF92_003866"
     CDS             complement(13593..14579)
                     /gene="yiaO"
                     /locus_tag="LAF92_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HBI9792796.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
BASE COUNT         3424 a         3714 c         3970 g         3558 t
ORIGIN      
        1 caggctgcac cctacacgaa gtagggacca ccaaccgcac gcacgcgaat gattatcgtc
       61 aggcggtgaa tgaaaatacc gcactgttga tgaaagtaca taccagtaac tacagcattc
      121 aggggttcac caaagcgata gatgaagcgg aactggtggc gctcggcaaa gagctggatg
      181 ttcccgtagt gactgattta ggcagtggct cgctggtcga tcttagccag tacggtttgc
      241 cgaaagagcc aatgccgcag gagttgattg cggcgggcgt cagtctggtg agtttctccg
      301 gcgacaagtt gttaggcggg ccgcaggcag gaattattgt tggtaaaaaa gagatgatcg
      361 cccgcctgca aagccacccg ctgaagcgtg cattacgcgc ggataaaatg accctcgcgg
      421 cgctggaagc cacgttgcgt ctttatttac accctgaagc tctgagtgaa aaattaccga
      481 ccctgcgcct gcttacccgc agcgcagagg tcattcaaat ccaggcacaa cgtttacagg
      541 ccccccttgc cgcacattac ggcgcggagt ttgcggtaca ggttatgcca tgtctttcgc
      601 agattggcag tggttcgctg ccggttgatc gcctgccgag cgcggcatta acgtttacac
      661 cccatgatgg acgcggtagc caccttgagt cattagccgc ccgctggcgt gaattgccag
      721 tgccggtgat tggtcgtatt tatgacggac gattgtggct ggatttacgc tgccttgaag
      781 atgagcaacg gtttttggag atgttgttga aatgattatt gcgactgccg gacacgttga
      841 ccacggcaaa acaaccttat tgcaggcgat tactggcgta aatgctgatc gtctgccgga
      901 agaaaaaaag cgcggcatga ccatcgatct cggctatgcc tactggccgc agccggatgg
      961 tcgcgtgcct ggttttatcg acgtacccgg tcatgaaaag tttctttcca acatgctggc
     1021 gggcgttggt ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg gcgtgatggc
     1081 acagacccgt gagcatctgg cgattttgca gctgaccggt aacccgatgc tgacagtggc
     1141 gctgaccaaa gccgatcgcg tggacgaagc gcgtgttaat gaggttgaac gccaggtaaa
     1201 ggaggtgctg cgggaatacg gttttgctga ggcaaaactg tttatcaccg cagcaaccga
     1261 aggtcgggga attgatgccc tgcgcgagca tctgcttcag ttgccggaac gcgagcacgc
     1321 cagccaacat agtttccgcc tcgctattga ccgcgcattt accgtaaaag gtgccgggct
     1381 ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta ggcgattcac tctggctgac
     1441 tggtgtaaat aaaccgatgc gtgtacgtgc gctgcatgcg caaaaccagc caacagaaac
     1501 cgccaatgcc gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa aagagcagat
     1561 taaccgtggc gactggctgc ttgccgatgt gccgccagag ccgttcacac gggtgattgt
     1621 cgagcttcaa acccatacac cgctgaccca gtggcagccg ctgcatattc accacgccgc
     1681 cagccacgtc acgggacgcg tttcactgct ggaagataac cttgccgagc tggtgttcga
     1741 cacgccgctg tggctggcgg ataacgatcg cttagtgctg cgcgatatct cggcccgcaa
     1801 cacgctggcg ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta aacgtaagcc
     1861 ggaatatctg caatggctgg cgtctcttgc acgggcgcag agcgatgccg atgcgttatc
     1921 tgttcatctg gaacgcggcg cggttaacct tgcggatttc gcctgggcgc gccagctcaa
     1981 cggcgaaggg atgcgcgaat tgctgcaaca gcctggctat attcaggctg gttatagctt
     2041 gttgaatgcg ccggttgccg ctcgctggca acggaaaatt ctcgacacat tagcgactta
     2101 tcatgagcaa catcgcgatg aacctgggcc tgggcgcgaa cgtctgcgac gtatggcgtt
     2161 gccaatggaa gatgaagcgc tagtactgtt gctgattgaa aagatgcgcg aaagcggcga
     2221 catcctcagt catcacggtt ggctgcatct gccggatcac aaagcgggct tcagcgaaga
     2281 gcagcaggcc atctggcaaa aagcagagcc gctgtttggt gacgagccgt ggtgggtgcg
     2341 tgatctggca aaagagacgg gtaccgacga gcaggcaatg cgcctgacgc tacgccaggc
     2401 ggcgcagcaa ggaataatta ccgcgatcgt caaagatcgt tattaccgta acgatcggat
     2461 tgtcgaattt gccaatatga tccgcgatct cgatcaggag tgtggttcaa cctgcgcggc
     2521 ggatttccgc gatcgcttag gcgtaggccg aaagctggca attcagattc tggaatattt
     2581 tgaccgcatt ggctttacgc gtcgtcgtgg aaatgatcat ttattgcgcg acgcattatt
     2641 atttccggaa aaataaggaa atgattaatt aatttttaaa ataaattaat acaaatttct
     2701 tatgatttta aaaaaagcac attgtttaat gaatacaatg tgctttttat tagattaatt
     2761 ttgataatca ataataatga tcatgttttc gaatgaaaat cctcagtaag ctgcccaccc
     2821 ttttttacac tttcaggagt gtgttatggc atcttcaact ttctttattc cttctgtgaa
     2881 tgtcatcggc gctgattcat tgactgatgc aatgaatatg atggcagatt atggatttac
     2941 ccgtacctta attgtcactg acagtatgtt aacgaaatta ggtatggcgg gcgatgtgca
     3001 aaaagcactg gaagaacgca atatttttag cgttatttat gatggcaccc aacctaaccc
     3061 caccacggaa aacgtcgccg caggtttgaa attatttaaa gagaataatt gcgatagcgt
     3121 gatctcctta ggcggtggtt ctccacacga ctgcgcaaaa ggtattgcgc tggtggcagc
     3181 caatggcggc gatattcgcg attacgaagg cgttgaccgc tctgcaaaac cgcagctgcc
     3241 gatgatcgcc atcaacacca ctgcgggcac ggcctctgaa atgacccgtt tctgcatcat
     3301 cactgacgaa gcgcgtcata tcaaaatggc gattgttgat aaacatgtca ctccgctgct
     3361 ttctgtcaat gactcctctc tgatgattgg tatgccgaag tcactgaccg ccgcaacggg
     3421 tatggatgcc ttaacgcacg ctatcgaagc atatgtttct attgccgcca cgccgatcac
     3481 tgacgcttgt gcactgaaag ccgtgaccat gattgccgaa aacctgccgt tagccgttga
     3541 agatggcagt aatgcgaaag cgcgtgaagc aatggcttat gcccagttcc ttgctggtat
     3601 ggcgttcaat aatgcttctc tgggttatgt tcatgcgatg gcgcaccagc tgggcggttt
     3661 ctacaacctg ccacacggtg tatgtaacgc cgttttgctg ccgcatgttc aggtattcaa
     3721 cagcaaagtc gccgccgcac gtctgcgtga ctgtgccgct gcaatgggcg tgaacgtgac
     3781 aggtaaaaac gatgcggaag gtgctgaagc ctgcattaac gccatccgtg aactggcgaa
     3841 gaaagtggat atcccggcag gcctacgcga cctgaacgtg aaagaagaag atttcgcggt
     3901 tctggcgact aatgccctga aagatgcctg tggttttact aacccgatcc aggcaactca
     3961 cgaagaaatt gtggcgattt atcgcgcagc gatgtaatca tcatttccac aacggctggc
     4021 aaattgtcag ccgctttttc aactatctct gtaacccttg cccgtaaatt cgtgatagct
     4081 gtcgtaaagc tgttaccgac tggcgaagat ttcgccagtc acgtctaccc ttgttatacc
     4141 tcacaccgca aggagacgat catgaccaat aatccccctt cagcacagat taagcccggc
     4201 gagtatggtt tccccctcaa gttaaaagcc cgctatgaca actttattgg cggcgaatgg
     4261 gtagcccctg ccgacggcga gtattaccag aatctgacgc cggtgaccgg gcagctgctg
     4321 tgcgaagtgg cgtcttcggg caaacgagac atcgatctgg cgctggatgc tgcgcacaaa
     4381 gtgaaagata aatgggcgca cacctcggtg caggatcgcg cggcgattct gtttaagatt
     4441 gccgatcgaa tggaacaaaa cctcgagctg ttagcgacag ctgaaacctg ggataacggc
     4501 aaacccattc gcgaaaccag tgctgcggat gtaccgctgg cgattgacca tttccgctat
     4561 ttcgcctcgt gtattcgggc gcaggaaggt gggatcagtg aagttgatag cgaaaccgtg
     4621 gcctatcatt tccatgaacc gttaggcgtg gtggggcaga ttatcccgtg gaacttcccg
     4681 ctgctgatgg cgagctggaa aatggctccc gcgctggcgg cgggcaactg tgtggtgctg
     4741 aaacccgcac gtcttacccc gctttctgta ctgctgctaa tggaaattgt cggtgattta
     4801 ctgccgccgg gcgtggtgaa cgtggtcaat ggcgcaggtg gggaaattgg cgaatatctg
     4861 gcgacctcga aacgcatcgc caaagtggcg tttaccggct caacggaagt gggccaacaa
     4921 attatgcaat acgcaacgca aaacattatt ccggtgacgc tggagttggg cggtaagtcg
     4981 ccaaatatct tctttgctga tgtgatggat gaagaagatg cctttttcga taaagcgctg
     5041 gaaggctttg cactgtttgc ctttaaccag ggcgaagttt gcacctgtcc gagtcgtgct
     5101 ttagtgcagg aatctatcta cgaacgcttt atggaacgcg ccatccgccg tgtcgaaagc
     5161 attcgcagcg gtaacccgct cgacagcgtg acgcaaatgg gcgcgcaggt ttctcacggg
     5221 caactggaaa ccatcctcaa ctacattgat atcggtaaaa aagagggcgc tgacgtgctc
     5281 acaggcgggc ggcgcaagct gctggaaggt gaactgaaag acggctacta cctcgaaccg
     5341 acgattctgt ttggtcagaa caatatgcgg gtgttccagg aggagatttt tggcccggtg
     5401 ctggcggtga ccaccttcaa aacgatggaa gaagcgctgg agctggcgaa cgatacgcaa
     5461 tatggcctgg gcgcgggcgt ctggagccgc aacggtaatc tggcctataa gatggggcgc
     5521 ggcatacagg ctgggcgcgt gtggaccaac tgctatcacg cttacccggc acatgctgcg
     5581 tttggtggct acaaacaatc aggtatcggt cgcgaaaccc acaagatgat gctggagcat
     5641 taccagcaaa ccaagtgcct gctggtgagc tactcggata aaccgttggg gctgttctga
     5701 tataagaagc tggtcgcatt gggtattcat tgcctgatgc gacgcttacg cgtcttatca
     5761 tgcctacggg aacctgaccg taggccggat aaggcgtaca cgccgcatcc ggcataaaca
     5821 aagcgcacgt tgttacaatt tacgttttaa acctacgtgc gcaataaccc agactacgcc
     5881 aacagaagtg cgtttctggg gcgtgttttg tcaggagttt aatctcgcgt ttcgatggtg
     5941 agcgggcgaa ccaggccgta aatcaccatt ggtgatccga ggccaatcag caataaacgg
     6001 tagataattg aaggtttaat acgcttaatc tgtactgtgg atgttttact ggagttattc
     6061 attagtttgg ccaatagttc cactatttca atatgataaa cctcgtcctg ttgaaaaacc
     6121 agacagcaca tttcagaaag tatataaaaa acaatatgtc gttatataaa taaggaaatt
     6181 gcactgcatc atggcatttc ataatatgcg ttaattgttc ttttttatat tctttgaggg
     6241 aaatatgttc ctggactatt ttgcactggg agtgcttatt tttgtatttc tggtgatatt
     6301 ctatgggatt attattttac atgatattcc ctacctgatt gctaaaaaac gtaatcatcc
     6361 tcatgccgac gccattcatg ttgctggttg ggtgagtctt tttacgctgc atgttatctg
     6421 gccatttttg tggatttggg ccacgcttta ccgcccggag cggggatggg gaatgcaaaa
     6481 ccatgactcc tccgttgtgc aactacaaca gcgtattgcc gggctggaga aacagctcgc
     6541 cgacatcaaa tcctcttctg ccgagtaata cttatggatt tactgattat tttgacctat
     6601 gtggcttttg catgggcaat gtttaagatc ttcaaaattc ctgtaaataa atggaccatt
     6661 cccacagcgg ccctgggtgg catatttatt gtcagtggtt taattctgtt aatgaactat
     6721 aaccatccgt ataccttcaa agcgcaaaaa gcggttattt ctattcctgt tgtcccacag
     6781 gtgacaggcg tggtgatcga agtgacggat aagaaaaata cgctgattaa aaaaggtgag
     6841 gtgctatttc gactggaccc gacgcgttat caggcgcggg tggatcggct gatggcggat
     6901 atcgttaccg cagaacataa acagcgggcg ttgggcgcag agttagatga gatggcggcg
     6961 aatactcagc aggcaaaggc cacgcgggat aaattcgcta aagagtatca gcgttacgca
     7021 cgcggcagcc aggcgaaagt aaacccgttt tcagaacgcg atattgatgt ggcgcggcaa
     7081 aattatctgg cgcaggaagc ctccgtaaag tcatcggcgg cggaacaaaa acagatccag
     7141 agccagctgg atagcctggt gttgggtgaa cattctcaaa tcgccagcct gaaagcgcag
     7201 ctcgcggaag caaaatataa ccttgagcag acgatagtgc gtgcgccgag cgatggttat
     7261 gtgacccagg tgctgattcg tccgggtacc tatgccgcgt cgctgccgct acgtccggtg
     7321 atggtgttta tacccgatca gaaacgacaa attgtggcgc agttccgtca gaactccttg
     7381 ctgcgactgg cccctggcga cgatgcggaa gtggtgttta atgctctgcc aggtaaggta
     7441 ttcagcggca agctggcagc cattagtcca gctgttcccg gcggagctta tcagtcgacc
     7501 ggcaccttac agacgttaaa cacagcgccg ggttcagatg gcgttatcgc gaccattgaa
     7561 ctggatgagc acactgattt gagcgcttta ccagacggta tttacgccca ggtggcggtc
     7621 tactctgatc atttcatcca tgtctcggtg atgcgcaaag tgctattacg catgaccagc
     7681 tgggtgcatt acctttatct cgatcattaa tcttcgcttt ccgccgcttg ttcaggccgc
     7741 agttgctgcg actgagcaag ctggtggcga atttcacgca gcatttcatc cagcacatac
     7801 tggacccgcc agggctggta ttcccgttta cggaagatag ccaccagatc cagccaccat
     7861 tcatacgggt gctgaaagca gggcaccagc gtgccgtctt gcaggtcgct ctgcacactt
     7921 ttgtctggcg caaacacaat ccccaggtga tttcgtgcca gctccagcgc agattgcgtg
     7981 ttgtcgcaaa cgtaatttcc cttcacgcga taatcccgca cctctttact cccggcgacg
     8041 ttgaagcgcc agatattcgc gtcgtcgatc atcatcgagt cgatcaaaat acaggagtga
     8101 tgttcgagtt cgtcaggacg gctaatggga tgtttctcaa gatagcgctg gctggcataa
     8161 gcggttacgg catattgggt aataaaactg gcaaccagcg attcatcttt tggtggggca
     8221 tagctgatta aaacatcaca atcatcagga aattcgacgc cttcataaaa ggcattacgc
     8281 tcaagattgc aggtttttag cgataaggtg atatcaccga tatctttaat tttatcgatt
     8341 acatgtttgg ataaataggt aattatgccg gttggggcgt agatggtgac ccgaccacgt
     8401 ttctcatgct tataatctgc aataaaatta ttaagctgct cgtttctgtc cagcatgtcg
     8461 ttgatgtacg gtaacagcgc ggtgccaaaa ggtgtgagca tcagttgtcg ggtggttcgg
     8521 tcaaaaactt ttaaacccac ttttgattca aaatcagcaa gatatttgct gacgttggcc
     8581 tgtgcgatgc caagtacagt cgcggcatgg ctgatatttt cactggcagc gattaccgag
     8641 ataattttta actcccggta cttaagttgt aattttgtca tcgcaacagc ccaattatat
     8701 ataattttat atattactat ataaatatga atcttgcacc ggagaaattg taagcattac
     8761 tatgccgact cttttttatc ttattttaaa atggaatacc ggacatgtta attaaccgca
     8821 atattgtggc gttatttgcg ttgcctttta tggcaagcgc aactgcttct gaattatcca
     8881 ttggtgctgg tgcggcttat aatgaatcgc cttatcgcgg ttataatgaa aatacgaagg
     8941 caattccgct gattagttat gaaggtgata ctttttatgt tcgtcagacc acgttaggtt
     9001 ttattctgtc gcaaagtgaa aaaaatgaac ttagcctgac cgcatcctgg atgccgctgg
     9061 aatttgaccc tgccgataat gacgattatg ccatgcaaca gcttgataag cgtgatagta
     9121 cggctatggc gggggttgcc tggtatcacc acgagcgttg gggaaccgtg aaagcctctg
     9181 cggctgcgga cgttctggat aacagcaacg gctgggtggg ggagctatcg gtattccaca
     9241 aaatgcagat aggtcgcctg tcgctgacac ctgcgctggg cgttctctat tatgacgaga
     9301 atttcagtga ctattactat ggcatttcag agagtgagtc ccgtcgtagc ggtctggcaa
     9361 gttattccgc gcaggatgcc tgggtgccct atgtcagcct gacggcaaaa tacccgatag
     9421 gagagcacgt cgtattgatg gcgagcgcag gatacagcga gctgccggaa gagattaccg
     9481 acagcccgat gattgatcgt aatgagagtt ttacctttgt caccggggtg agctggcgtt
     9541 tttaattcac cggtggatgt cggtgcggct ggtgctccgt cgtaatgcca caaaggttgt
     9601 tgctctggat tgctctgttc ataccgggaa cctggcgtct gaatcaggat cttatggctg
     9661 aacaatccct tgccttcaaa tgtcgtaaat ggcgcatcgg cgggcagcaa caggttatgc
     9721 agtgactcga cgttgtcggc ctgctccatg caatacggtt taatgacgtt ggctatttcc
     9781 ggcagatttt tcagaaacat actggtttaa cgccagcagc gtggaagcgg cgttacagca
     9841 aggaacatat caattcgtag tgccggggcg atgaagcccc ggcgtgaggg attactgccc
     9901 gtaataggca ttggccccat gcttacgcag gtagtgctta tccagcagtt cgttttgcat
     9961 cgcagggagc tgcggcgcaa gctggcgcga gaatagaccc atataggcgc attcttcgag
    10021 tactacggcg ttatgcacgg catcggcggc gtttttaccc catgcgaacg ggccgtgaga
    10081 atgcaccagc accgccggga tttgtgccgg actcctgcca cgttcttcga aggtttcaat
    10141 gatcacttcg ccggtctgat attcatattc gccgttaatc tcctctgcgg tcatctgtcg
    10201 cgtgcagggg atagcaccgt aaaaataatc ggcgtgggtg gtgccccagg cggggagatc
    10261 cagcccggcc tgtgaccaga tggtggcgtg gcgcgagtgg gtatgcacaa tgccaccaat
    10321 ttcggcatag cgacggtaaa gcgccagatg cgttggcgtg tcggacgagg gctttttgct
    10381 accttccacc accttgccgc tggctatctc aaccaccacc atatcgtcgg cggtcatcac
    10441 gtcgtactcg acgccggaag gtttgattac catccattgc cgcgtgtcgt ctaccgcgct
    10501 gacattgccc caggtgaacg tcaccagatg gtgagcggga agcgccagat tcgccgccag
    10561 cacgtcggct ttcagttgct ctaacatata aatccagcct cctgcatacg cgcttcaatc
    10621 cagcgccgcg cctgaatgat ctccagcacc ggctctttgg ctttttcggt ccacatctca
    10681 atcaggaaag aaccgcgata gttcagttca tgcagcgttt tgaagatgcc aacgaaatcg
    10741 acgcagcctt cgccaaacgg cacatcacgg aactgtccgg ggctttgccc ggtgactggc
    10801 tgggtgtctt tcaggtggat cgcggcgata cggtcaatgc ccagtttcag ttcggcggga
    10861 acatcattgc cccaggcgct gaggttgccg acgtccgggt agacggtgaa ccacggcgag
    10921 gcgagcatct cgtcccattt tttccatttg ctgatggagt tcataaacgc tgtatccata
    10981 atctccaccg ccagcattac ttgtgatgcc gccgcctgtt cgactgccca cgccagccct
    11041 tcagcaaaac gttgccgggt gccttcgtcg tggtcttcgt aatagacgtc ataacctgcc
    11101 agctggatgg tgcgaatgcc gagatcgcgc gccaggcgaa tcgctttact catgatttcc
    11161 cgcgcccgtt cgcgcactgc ctcgtcacgg ctaccaaaag gaaagcgacg atgtgcggac
    11221 aggcacatcg acgggatccc aacacccgtt tcgatcatcg cggcaaccag cgaagtcctt
    11281 tgtgcggtgc tccagtcaag acgtgaaagc cgttcgtcgg tttcatccac cgacatttcg
    11341 acaaaatcaa aaccgcagct ttttgccagc accagccgct ccggccagga gagatctttc
    11401 gccagcgcct tttcataaat ccctaacgga tgattacgca cgcttgcctc cccagatagc
    11461 gtcgatttgc gcatggaaat cgccagccac ctgcgccgga tttgctgagc ctgccagtgc
    11521 tcgcccggca ataaacgctt tcacgcgaat atctttaaac agcggcaggt cagcaggggt
    11581 aatcccgccg gtaatggaaa gctcaaggcc gatatcggaa agcgccttca tgcgtgccag
    11641 atcggcttcg ccccactgtt gcccgctggc ctgtgcatca cgaccgcgat gataaatcgc
    11701 ctgccgcacg ccaatacgat gccagtcgcg ggcgtcgtcc agcgtccagt taccgaacag
    11761 ctcgatctga atttcaccac cgcagcgttg cgccatcgcg tggccttttt ccaccgtcgc
    11821 gagcggtgcg gcgcagatga tggtcatcca gttagcgcct gcgccaaatg cctgttgcgc
    11881 gagcgtttca ccagcgtcgg cgaccttcca gtcggcaacg atgattttgt cagggcactg
    11941 ttcgcgcaag gctttcaccg cgccaagccc ttcgtttaaa cagagaatgg tgcccgcttc
    12001 gacaatatcg acgctgtctt ttaactgcgt cacgtcgcgc tgtgcggctt caagtgatga
    12061 gtggtcgagt gccagttgca gaagtggtcg gctcataata tgtgctcctt gatgcgggcg
    12121 tgaaagccct gaagtgcggc aatgagatgc tgatagcgtt ggtatttttg ttggtaaagc
    12181 tgatggacag tcatatccgg cagcagggtg cgcaccgggt gctgtaaatc acgctgggct
    12241 tcgctgaaat cgcgataaac cccggtgccg acgcgggcgg caagtgctgc accaaagcag
    12301 ccggtttctt ccacctgcgg cagctcaata cgcagaccgc tgacgtccgc cagcatttgc
    12361 atccagacat cggaatgcgc cgggccgcca gtgacgcgca gggtgtgcac atcagtaaaa
    12421 cgttcgcgca tccggttgag atgggtcata tggctgaaca ccaccccttc ataaatggcc
    12481 tgcaacaggt gtgcgcgggt gtgaatggcc tgcatcccgt agaaaccgct ggtcatctcg
    12541 agtccggcgt tgctgccgta caggaacggc aggaaaaaga gatcgccacc ggccttcggc
    12601 aagctggcaa cggcctggtt gatctcatca aacgagattt ctccccactg tgcggtaaac
    12661 cattcgaggt tgccggaaga ggtggggctg gcttcgtgaa cgataaattc accatcgtta
    12721 acgtagcgac catagacata cggatgcgct tcaccgtcac gtaaaccgcg ggttatgccg
    12781 ctggtcaccg cccaggtgcc catcaccgca ttaagggtaa attcgtcttc gatcccggcg
    12841 cagagtgcgg tggaaaccac atcaaacagg ccgccgacaa cgggcgtacc cgctttcaga
    12901 ccggtcagta cggctgtctg agcggtgatc tccccgcaga tttcggcaga tccgacaaca
    12961 ggcggcaggg cgtgattgat ttcagcaatc cccagccagt cggtgaggca cgggtcatat
    13021 tccccaagac tcatgttgta gaggttggac tcggaaatat tgctctcttc acagcctttg
    13081 acgccggtta aacaccagcg caggtagtcg tgcgtcatca tcacgcagcc aatttgcgcg
    13141 tagcgttccg gttcgtgctc tttcagccag cgtaacagcg acaccggatg cccggtccac
    13201 aaggtttgtc gggtcaacgg gtagagtttt tccgggatgc catcttcctg ccagcgacga
    13261 acgatttcca tcgcccggcg atccgaggac aaaatggcgt taccgagcgg cttgttgttt
    13321 ttatccagca aaaacaagcc ttttccttgt gcggagatac cgataccgac aatttgttcc
    13381 ccgctaacgt cggaatgggt aagcagttcg cgaatgacag ccatgcagca ttgccacagt
    13441 tctgccatat cgcgctctgc ccaacctggc tgcgggctta atgcgcacag cggcaggcgc
    13501 tgcacgcctg cctcccggcc ttcgcggtca tacagcccgg ctttcagcca gctaccgcca
    13561 caatctaacc ccagccagta ttgcgtcatg gtttattgca cctcatccac ggctttcagt
    13621 agatcagcac cctgcggaac atcttttaca aacatgtcgc ggacctgagt acccagtgcg
    13681 tcgctaaagg ctttgcggtc gagatcggtg atgacttcca cgcccgcttc tttcatgccg
    13741 tcgatgattt tttgctgatc ttcggcaacc agtttgcgct ggtagttacc cgcttcctgc
    13801 gcagatgaaa ttagcgcttg ctggaactcc ggggttaagc catcaaactt cgctttgttg
    13861 atcaccacca aaagcgggga ataggcgtgg tgcgtcagag aaaggtactt ctgcacttcg
    13921 aaaaattttg ctgaccagac gacattgatc gggtgttcct gagcgtcgat agtgcgcgtt
    13981 tccagcccgg tatagacttc ggcaaatggc atcgggatcg ggttagcgcc aaagactttg
    14041 aatgcggcga tattcatcgg gctattgttg gtgcgaattt tcagcccttt caggtcggcg
    14101 ggggttttaa ccggtgcgcg cgagttggtg acatcgcgcc agccgttttc ccagtaggcc
    14161 agtactttca gtcctttacc ttcaagtgag gctttcagat catcaccgac tttgccgtcg
    14221 agcgttttat gcgcgtgagc ggtatcgcgg aacaggaaag ggacatcaag caagttcatc
    14281 actggtgata acccggcaaa gttattcgag ccggacattt ccatatcgat ggtgccgcca
    14341 cgtacgccgc tgatcatcgc ctgcgcgtta ccgagggtgc tatccggaaa caattttaat
    14401 ttcagctcgc ctttggttct ctcctgcaat aaatcattga attttttcgc cgcaatatgt
    14461 tgcgagtcgg tttgtgaggt ttcataaccg aaacgtaaag attgcgccgc cagagaagat
    14521 gtgctgaatg cagccaggcc agcaatgaat aatgcgtagg ttacagagcg taatttcata
    14581 atattttcct tttaattaat ccatttcaaa gggaggatga tgagatcggg aataaaaaca
    14641 aatacgacta ataatgaata taaaac
//
LOCUS       DAEVZA010000123        14493 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEVZA010000123 DAEVZA010000000
VERSION     DAEVZA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.123"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..639)
                     /locus_tag="LAF92_003867"
     CDS             complement(<1..639)
                     /locus_tag="LAF92_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBI9792797.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQL"
     gene            complement(655..3135)
                     /locus_tag="LAF92_003868"
     CDS             complement(655..3135)
                     /locus_tag="LAF92_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI9792798.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(3151..3825)
                     /locus_tag="LAF92_003869"
     CDS             complement(3151..3825)
                     /locus_tag="LAF92_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI9792799.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVSRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(3906..4448)
                     /locus_tag="LAF92_003870"
     CDS             complement(3906..4448)
                     /locus_tag="LAF92_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9792800.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            complement(4741..5022)
                     /locus_tag="LAF92_003871"
     CDS             complement(4741..5022)
                     /locus_tag="LAF92_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBI9792801.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(5285..6394)
                     /gene="apbC"
                     /locus_tag="LAF92_003872"
     CDS             complement(5285..6394)
                     /gene="apbC"
                     /locus_tag="LAF92_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBI9792802.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            6526..8559
                     /gene="metG"
                     /locus_tag="LAF92_003873"
     CDS             6526..8559
                     /gene="metG"
                     /locus_tag="LAF92_003873"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBI9792803.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            8700..12494
                     /locus_tag="LAF92_003874"
     CDS             8700..12494
                     /locus_tag="LAF92_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HBI9792804.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWVRGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            12504..>14493
                     /locus_tag="LAF92_003875"
     CDS             12504..>14493
                     /locus_tag="LAF92_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792805.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPAQTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIATLPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVANDPTAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQTQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDL"
BASE COUNT         3920 a         3615 c         3282 g         3676 t
ORIGIN      
        1 taattgtgaa acgggaatac tgtctaaagt aattaataaa tcgtccggtt tgcaggttgt
       61 ggttatcggg ttgtatgaca atgtcagatt aaaggaatag acacctgcat tttgtgccaa
      121 tgcatttctg tagctattta cacatgcatc ccatgacttg ccacctaata aacacagtag
      181 aaattgtact gcggcactcc cgcgtgtcga tgatgaagca ctggataatg tcgcaacacc
      241 attaatcatc actgaaccgc cgccccccgc agaaacattc acttttgcat tactggccgc
      301 aggtgtcacc gtaactgaat aaccactggt gaaactggtc tgattatttg acaacgtgat
      361 gttgttttta ttggtccatt caaaatgtaa atttaatttt atggtatcgt tataaagtcc
      421 aacaataata tccgacgggc tcataggggt gatagtattt gtacttacgc atgacgaatc
      481 attaaaatta tgctcatatg tcgccatttg tgtttgagta agtgcaaaat ccccctgaac
      541 aaccaagttg tgctgaacag ataactcact ataacatgcc gcatagctca caggcataac
      601 catcatcatt aatataaata gcattatgcg aatttccata gaaccacctg actattactg
      661 gcaaatataa tttctacttt catcaatctc tttaccgaag gtgattgtgc atgaaagtcc
      721 ttgttgctta tcaattgcca cattaaccga tggcggtact tcattggtgc gaataaataa
      781 ctggcttccc tggccgacaa cgccaatatt atgaccatgg atatcattga cttcataacc
      841 aaacattaat ggttgaccat ccgctcttaa cgctttaata aaccagggct tgcgctgatc
      901 ggtatcaaaa ttaaccagta caaccgcgcc gcgataaggg gcggcaattt tccggttgcc
      961 acgtaattct gcttcgctat cgctttgcga cacatccagc atcaggtgat tttcccgata
     1021 aggtgtcatt ccgtcgtata ccaccactcc attacggttt gttgtgcgat atttttgccc
     1081 attgacataa gcatctttaa ttcctggcgc attcatcaca gcaaacgttt cggaaagacg
     1141 gttcgccaga ttaacgcccc ccgaccaggc gacaatgccc cctgaaacac tggctccagc
     1201 ctgtcgataa gtactcgact gactataact gccattcact gtcgcaaccg gcgcgttcca
     1261 ggtcaaattc gccccagctg tcgtttcatt tccctgattc tgataactca ggttgacccc
     1321 atagttaaac tgatcccggc ttccaacggt tcctgataat cccgtattat ttgaggcaaa
     1381 cccctgatca tcaaacgtcg ttgagttaga catatatatt tgccgacgag gcgtcgtaac
     1441 gtcatcaccc caatcaaagg gaatcgatat aaaaatatta aaacgtttct cttcatgatg
     1501 attctcgtca tacgcctggc ttgccgcgag gatatagctt atccgccgcc agttattgga
     1561 ataactcaac tgataatcct tactgctgcc gctacgcccc cagtaatctc gccataacgt
     1621 acttaaggac acagaccccc agccttctgg caatgactgg ctcatattgg cagagaagct
     1681 atttttacgg ccaaaatcgt tctggtaata atcggcaatg tcatagacat cgttttcatc
     1741 acggcgataa ttatctttat tgtttgccca aacgtgatca ttaaatgtcc ggtaatcacg
     1801 cgatgaataa cgccaggccg ccagaccaaa acgcgtcgac gtttggctca caaatttgtt
     1861 gtaggcaatt tgataacttt gcccgtcaaa cacatcgccg ttgtcttgtt tactatgcga
     1921 cttcgtggca tcgacggaaa tggcaccaat gcgtgtattc cagccagtcc ccaaagtaaa
     1981 cgcgtaataa ttattcgcga ccatcgagcc accatacagc gtcaataaat tattaaaacc
     2041 atactgataa cccgcctgga caaaatcact ttgtttgctc gccccttcaa tatggctacg
     2101 acccgccgca aaatcatatt tcgatacgcc gggttgcagc atatttggca ctgctgcata
     2161 aggcaccaga taggtggtta ccgagccgtc cgcctctttc acgctgacat caagatctgc
     2221 tccaccaccg gccaactgca aatctgtaat cgcgaacggg ccaggaggaa cctctttctg
     2281 ataaaccaca aaaccgttct gttcaatagt taccagcgca ttactctgag caatcccctg
     2341 cacccgtggc gtaaaatttt gtttcgagtt aggcaacatc tgcatatcac gaaacaaccg
     2401 cacaccgctg aagcgaacag aatcaaaaat atcgcttgat gtgtacatat cacccacgcg
     2461 aagcgtgccg agaagttggg caaatccacg ttccagatac agggtattgc ttttccacac
     2521 ccctggattg ttatttgttt tactgaaact ggcatcagaa tgcagttgcc accccagtaa
     2581 atttaacccg ctgttaaaac gtacatatgt actcttacta ttacccgacg ctttatagtc
     2641 actgtaatac tgactcacat aataagaggt ataaaacgca ttaatacccc gctcccagtt
     2701 ttccggtgga acatagccac tttccagttc ttccacccag gcctgcggga cactgaaatc
     2761 gagacgaaaa accccgatat cccaggtata gctcccaccc tgaacaagtt gctcaaatgt
     2821 taaacattgc ttaccgctgg cgaagttatc gctattaatg cctaaccgct tgataacttc
     2881 tattgataaa catgtttctt gcgggttgtc tttaacaata atctcatatt tcccgcgcca
     2941 ttgcttattg acataaatat cgatgtcata ctgccccggt aagggttgat tatcatcaag
     3001 acgaatattt gcaacctgct ggtctttcat tccacctatc ataaaatggg tatcaaaggt
     3061 ttcttcagct gcataagctt caatgccgag caataacgct acgattgctg atgcaagtgg
     3121 ggtcattctc aacataggca gctcctgcaa ttaaattttg tcactaatat agttgccgtg
     3181 gtcatcgata atggtcagat accagttatt tgcattatta cttttgacat taacactctg
     3241 actttctaag ggggcaatca ttatagtttc ataattgact ttgacattat tagctttgac
     3301 atccgaaatc gtcacccaat tagctgagtc attttttatc accaatccat tgccactgcg
     3361 acttaccagt aactttttaa aggtcgcttt atttaccgga gcaatacccg ccgggcggta
     3421 aaacaatttg attctgtttt gcatcgcaaa cttcagtgca ttcttacctt cttgttctgg
     3481 actatttggt ggaatatcca gaacattcag ataaaaaatg ctttctttat tagtgggcag
     3541 tttattcggc ataattttga tttttacttg ctgcccggaa tttgccccta tttttgccac
     3601 tggtggcgtt aacatgaaag gaacctgaat tttttctggt ggtaacgacg tatcgccatc
     3661 atcaatccac gcctgcagca gcgaagaacg gtttccctgg ttcatcaact gcaccataac
     3721 ttctttattt tctgccgggt aaataatgcg cgtcccgtag ataacaattc cggcatgtgc
     3781 aggaaggacc atagaaaaaa tgagtaaaga taataatcct ttcatcttaa tgcctcttaa
     3841 attaaaaagc cgccatgggg atagcggcca tgtttattac ctggagaaac ttgcaatttt
     3901 atttattaat cctgcgccac acgcataacc agagaagata acacatcacc tgattgcact
     3961 gagttatctg tcagtttcag gtattttgct ttaaacggaa tagcatattg accttccgct
     4021 gttttataac tctcaatgtt aaaaggtgca gaaacatcca ggacatcgct gttatcacct
     4081 aaatagagat agtaacccac ggtatcgttg ctactggcta tcgccttatc gttaccggtt
     4141 attttatcgt tggtaatcaa cgagagtttt acaccagcaa cttctttcgg gcagctaatt
     4201 tttaacatgc tgctggttac accgactgca tcatcatagg tagaatattt agctttacct
     4261 tcaaagacag tggttggtac agtcggcata ttaatactta ctgaatcacc acccgcttcg
     4321 aatttacacg gagattctgc aatattcccc ttaatagtta atgttccggt atcaacgtct
     4381 gcagcaaata ctccagcgct cataaaaaaa gaagaaaaga cagcggcagc aataattgaa
     4441 cgtttcataa tatgttcctt aataaaattt atttttggtt gttaagagtc tgtatctctt
     4501 ggaaacaaat ttctcacatc aaaaaagttg ccgcaatgta aacacattgt tttattgatt
     4561 taaaacaaat taaccattgc aatataaata caacaaatat ttaagagtat taactattta
     4621 tcgcatctat caattaatgt agatttatgt aaatggtatt gttgatatca ataaaaaagc
     4681 cgaaatcatt tatataaatg atttcggctt tctaaatgca attaaaatca ggttgccaaa
     4741 ttaatcgtag cgatttaaca cgattcgacg tttgctatcg ctacctgcaa caaccacttc
     4801 tgtcgttaca cctttgacgg gcgaagaaaa cagatgacgg gtgtcaacat tatacgttaa
     4861 ctgaccacaa tgctgttgtg gttgactatt aaccatagcc atactgcatg ttgggggact
     4921 gatcctgcca ttaatcgtta atgtggcagt ctctgaagag aatgcgggag aagccaatcc
     4981 ataggcaaga aaaataattc ctgacagcca atacttattc attgaacgtt atccctgtag
     5041 taaaggttat gcctggatag aatgagtgca taacaaacta tagctgtaca tccactacac
     5101 agccacgaag gatgataatg aagcattgcc tgtatgatca atcgactttg tagagtttcg
     5161 gacgaaggtc cgcagaatat tcgcagtatt aaataagtgt tcattaatgc ggcaaaaact
     5221 aatacaccgc atcaatgtaa catctctgcg gtgtatgaag aataaccaga atggttaata
     5281 ggcgttagac cgcgcggaag gaaatctcgc ctggaatgac ttcaccctgc cagtagagct
     5341 gagctgcaac gcggtcagcc agctggcggt agatagcggt aaattcgctc tccggacggc
     5401 tgataaccgt tggcgtgcct ttatcgagat cctcacgtaa ggaaatgtgt aacggcatct
     5461 gccccagcag ctgtgtgtgg tatttttcag ccagtttctc tgcaccaccg gtaccgaaaa
     5521 tcggttcatg gtgaccacag ttactgcaaa tatgcacact catgttctcg acaataccaa
     5581 gcaccggaac ctcgactttc tcgaacatca caatgccttt tttcgcatcg atcaacgcga
     5641 tgtcctgcgg ggtggtgacc acgactgcac cagttactgg aatgttttgc gccagcgtca
     5701 attgaatgtc accggtgcct ggcggcatat caagcaccag atagtcgaga tccggccaca
     5761 aggtttcctg caacatctgc attaacgcct tgctcgccat cggtccacgc cacaccatcg
     5821 cattgtcgtc agtgaccaga taaccaattg aattggttgc caggccatga gacataatcg
     5881 gtgccatatg ggtaccgtcc ggcgaagttg gacgctgatt ttctgcgccc agcatggtgg
     5941 gaattgatgg gccgtagata tcggcgtcca gaatgccgac tttcgcccct tccgccgcca
     6001 gtgccagcgc caggtttacc gccgtggagg atttacccac gccgccttta ccggaactga
     6061 ccgcaatgat gttcttcaca ccattaatac ccggctgatt cttcacgcgt tttaaggtgg
     6121 cgatattgtg cgacagtttc cagtcgatag ctttcgcccc ggtgatacgc agcagttcgg
     6181 cgctacattg ctcttttaac tcttcaaacg cgctatgcca gacgaatggc ataaccagtt
     6241 ccacatgcaa cgtgtcgtcc atccaggcga catggtgcaa cgctttcagc gtggtgaggt
     6301 tatgcttcag ggttgggtgc tgaaaattgg ccagtgtccc ggcgaccatt gcgcgcaagg
     6361 cttctggtga tttggcctgg gattgttcgt tcatcccgac tccttttttg tatagataaa
     6421 ccatcagctg atagtttacc tgaagaatat agagaagtac ttacttaaca ttttcccatt
     6481 tggtactatc taaccccttt tcactattaa gaagtaatgc ctactatgac tcaagtcgcg
     6541 aagaaaattc tggtgacgtg cgcactgccg tacgctaacg gctcaatcca cctcggccat
     6601 atgctggagc acatccaggc tgatgtctgg gtccgttacc agcgaatgcg cggccacgag
     6661 gtcaacttca tctgcgccga cgatgcccac ggtacaccga tcatgctgaa agctcagcag
     6721 cttggtatca ccccggagca gatgattggc gaaatgagtc aggagcatca gactgatttc
     6781 gcaggcttta acatcagcta tgacaactat cactcgacgc acagcgaaga gaaccgccag
     6841 ttgtcagaac ttatctactc tcgcctgaaa gagaacggtt ttattaaaaa ccgcaccatc
     6901 tctcagctgt acgatccgga aaaaggcatg ttcctgccgg accgttttgt gaaaggcacc
     6961 tgcccgaaat gtaaatcccc ggatcaatac ggcgataact gcgaagtctg cggcgcgacc
     7021 tacagcccga ctgaattgat cgagccgaaa tcggtggttt ctggcgctac gccggtaatg
     7081 cgtgattctg aacacttctt ctttgatctg ccctctttca gcgaaatgtt gcaggcatgg
     7141 acccgcagcg gtgcgttgca ggagcaggtg gcaaataaaa tgcaggagtg gtttgaatcc
     7201 ggcctgcaac agtgggatat ctcccgcgac gcgccatact ttggttttga aattccgaac
     7261 gcgccgggca aatatttcta cgtctggctg gacgcaccga ttggctacat gggttctttc
     7321 aagaatctgt gcgacaagcg cggcgacagc gtaagcttcg atgaatactg gaagaaagac
     7381 tccaccgccg agctgtacca cttcatcggt aaagatattg tttacttcca cagcctgttc
     7441 tggcctgcca tgctggaagg cagcaacttc cgcaagccga ccaacctgtt tgttcatggc
     7501 tatgtgacgg tgaacggcgc aaagatgtcc aagtctcgcg gcacctttat taaagccagc
     7561 acctggctga atcattttga cgctgacagc ctgcgttact actacactgc gaaactctct
     7621 tcgcgcattg atgatatcga tctcaacctg gaggatttcg ttcagcgtgt gaatgccgat
     7681 atcgtgaaca aagtggttaa cctggcctct cgtaatgcgg gctttatcaa caagcgtttt
     7741 gacggcgtgc tggcaagcga attggctgac ccgcagttgt acaaaacctt cactgatgcc
     7801 gctgaagtga ttggtgaagc gtgggaaagc cgcgaatttg gtaaagcagt gcgcgaaatc
     7861 atggcgctgg ctgatctggc taaccgctat gtcgacgagc aggctccgtg ggtggtggca
     7921 aaacaggaag gccgcgatgc cgacctgcag gcaatttgct caatgggcat aaacctgttc
     7981 cgcgtgctga tgacttacct gaagccggta ctgccgaaac tgaccgagcg cgcagaagca
     8041 ttcctcaata cagaactgac ctgggatggt atccagcaac cgctgctggg ccacaaagtg
     8101 aatccgttca aagcgctgta taaccgcatc gatatgaagc aggttgaagc actggtggaa
     8161 gcgtctaaag aagaagtgaa agcagctgcc gcgccagtta ctggcccact ggcagatgat
     8221 ccgattcagg aaaccatcac ctttgacgac ttcgcaaaag ttgacctgcg cgtggcgctg
     8281 atcgaaaacg cagagtttgt tgaaggttct gacaaactgc tgcgcctgac actggatctc
     8341 ggcggtgaaa aacgcaatgt cttctccggt attcgttctg cctacccgga tccgcaggca
     8401 ctgatcgggc gtcacaccat tatggtggcc aacctggcac cgcgtaaaat gcgctttggt
     8461 atctccgaag gcatggtaat ggctgctggt cctggcggga aagatatctt cctgttaagc
     8521 ccggatgctg gtgctaaacc aggccatcag gtgaaataat ccccctttaa ggcgctgcat
     8581 cgacagcgcc ttttctttat aaattcctaa agttgttttc ttgcgatttt gtctctctct
     8641 aacccgcata aatactggta gcatctgcat tcaactggat aaaattacag ggatgcagaa
     8701 tgagacactt tatctatcag gacgaaaaat cacataaatt ctgggcagtt gagcaacagg
     8761 gaaacgagtt gcatatcagt tggggaaaag ttggcaccaa agggcaaagc cagataaaaa
     8821 gtttttcaga tgctgcggca gcggcaaaag cggagcttaa gctgattgcg gagaaggtga
     8881 agaaggggta tgtggagcaa gcgaaggata attctttgca accttcccaa acggtaacgg
     8941 gctctctcaa ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag
     9001 aaacccgcgc gcctgacaaa aatacagatg ctgttttacc gtggctggcg aaagatattg
     9061 ctgtcgtttt tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag
     9121 ttcctgttca gcaagcagac aaattgaccc aattacgtcg cttagcctgt agtgtgtcgc
     9181 aacgggataa taaaacagcc acatttgact tcagcgcctg ttctttagaa tggcaaaaca
     9241 ccgtcgccca ggcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcaccag
     9301 taatggctgt actcacggca cttgaaatga aatgcacaag atataaagtg cgtgaggatg
     9361 ttatggatca gatcgtccag gaaggcggtc tggaatatgc tactgatgta ataatacacc
     9421 ttcaacagat tgatattaaa tgggattatg cgaataatgt cattattatt ctgccgtctg
     9481 gcattgcacc tgactacttg gagcaatatt ccagatttga attacgccta cgtaaacatt
     9541 tatcactggc ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc
     9601 cacatattcc agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa
     9661 ttgcacatga aattgcccag cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt
     9721 taaaaatagt ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat
     9781 atgccatttt tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc
     9841 aaggtgttgc agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg
     9901 tgttgcgtca tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata
     9961 ctaaacgctg tcacgatcgg atgacgaaag cctgcgctgc gttcccacat gcagcaatgg
    10021 cggcactgac ggaacttctt gggcaaaaag aagagaacag ttggcgcatt atgctaatga
    10081 caatgcttat ctcacaacca gcactggcag aacaggtcat tccctggctc tcgacacccg
    10141 cagttgccgt actgaaatca tgccagcaac aactgacaca gccctcaaac catgccagcg
    10201 ccgatctact gccagccgta gtagtctccc ctccctggct ttcgaaaaag aaaaaatcgc
    10261 cgattccggt gctggattta gcgccattag gcattgagcc aatctgttat ctgacagaag
    10321 aaatcagtaa tcaacttttg gcgaaatata tctggtattc aaaacacatc acggttagcc
    10381 atgaagaaag tactaccaac ctgttggcaa ggatgggttt tcaacgacgg atcgctggta
    10441 catatattaa agctcccgaa gcggtagttg aggcatggct aaatgaagat tattcaacct
    10501 tactaagtga atttaaggtg tttcattcac ctaccgggca ttattggcag ttggggattt
    10561 tgacaacatt gccgctggag aaagcagtaa aagcatggaa tgcccttacc ctatctccac
    10621 ataccgatac cgaatacgcc atgttacatt ttggactcaa agggttacct gggttagtaa
    10681 actcacttgc acgctatcca caagaagcct tgcccatcac gaattacttc gcagcgagtg
    10741 agctggctcc tgccgtcgcc cgtgccttca acaaactgaa aacgctacgc gaaaacgccc
    10801 gtagctggct gttgaaatac ccggaacatg cccttaccgg cctgctgcct gcggcgctcg
    10861 gcaaagccgg tgaagcacag gataacgccc gcgctgcctt gcgtatgctt accgaaaacg
    10921 gtcatcagcc attactgcaa gaaatcgccc gacgttataa ccagccggaa gtaaccgatg
    10981 cggtgaacgc tctgcttgcg ctcgatccct tagataatca cccgacaaaa atccccactc
    11041 ttccggcctt ttatcagcca tcgctctgga cgcgcccggt attaaaagca aatgcccaat
    11101 cactgccaga tagcgccctc ctccacctcg gtgaaatgct ccgcttccct caggaagagg
    11161 ctctgtatcc gggattattg caggtgaaag acgtctgttc cgccgactca ctggcgggat
    11221 ttgcctggga tctgtttacc gcctggcaga ccgctggcgc gccgtcgaaa gagagttggg
    11281 cgttcactgc gttaggcgtt ctcggtaacg atgacaccgc ccgcaaactg acgccattaa
    11341 tacgcgcctg gcctggtgaa tcccagcata aacgcgccac cgttgggttg gatattctcg
    11401 ctgctatcgg tagtgatatc gcccttatgc agcttaacgg catcgcccag aaactgaaat
    11461 tcaaagcatt acaggagcgg gcaaaagaaa aaattgccga cattgccgag agccgcgaac
    11521 tcacggtggc ggagcttgaa gatcggttag caccggatct cggtctggat gataacggtt
    11581 cgctgctgct ggattttggc ccacggcagt tcaccgtcag ctttgatgaa accttaaaac
    11641 cgtttgtgcg tgatgtttcc ggcagccgcc tgaaagacct gcccaaaccg aacaaaagcg
    11701 atgatgaaac gcgggcgaac gatgcggtta accgctacaa attgctgaaa aaagatgcgc
    11761 gtaccatcgc cgcccagcag gtagcaaggc tggaatccgc catgtgcctg cgccgccgct
    11821 ggtcgctgga aaacttccag ctcttcctgg ttgagcatcc gctggttcgt cacttaaccc
    11881 gccgtctgat ttggggcgtt tatagcgccg aaaaccagct actggcttgc tttcgcgtag
    11941 cagaagataa cagctacagc accgctgacg atgatctttt caccctgccg gaaggcgata
    12001 tctctatcgg cactcctcac gttctggaaa tatcaccaac ggatgctgcc gcctttggtc
    12061 agctttttgc cgactacgaa ctgctaccac cgttccgcca gctcgaccgt aacagctacg
    12121 ccctgacaga agccgagcgc aatgccagtg aactgacccg ctgggcaggc agaaaatgcc
    12181 cgagcggtcg ggtcatggga ctggcgaata aaggttgggt gcgtggtgaa ccgcaggatg
    12241 gtggctggat cggctggatg atcaaacctc tgggccgctg gtcgttaatc atggaaatcg
    12301 atgaaggctt tgcagttggt atgtcgccag ccgaacttag cgccgagcaa ctcttaagca
    12361 agctgtggct atgggaaggc aaagcagaaa gctatggctg ggggagtaat tcaacacagg
    12421 aagcgcagtt ctccgtactc gatgccatca ccgccagcga gctgattaac gatattgaag
    12481 ccctgtttga ataaggaaag agcatggaca aggaattacc gtggctggcg gataacgccc
    12541 aactggaact gaaatataaa aaaggcaaaa caccactcag ccatcgcaat tggccgggcg
    12601 aaccagtgcc tgttatcact gaaagcatca ttcagacact gggtgatgaa ttgctacaaa
    12661 aagctgagaa gaaaaaaaac attgtctggc gttatgagaa tttttcactg gagtggcagt
    12721 ccgccattac gcaggccatc aacttgatcg gcgaacacaa accctcaatc ccggcccaga
    12781 caatggcggc gctagtctgt atcgcgcaaa atgacagcca acagttgctc gacgaaatcg
    12841 tccaacaaga ggggctggaa tatgcgactg aggtggtgat tgcacgccag tttattacac
    12901 gatgttatga gagtgatcct ctggtagtca cattgcagta tcagaacgag gactatggct
    12961 atggttatcg ctcagaaacc tataacgaat tcgatctccg actgcgtaag catctctctc
    13021 tggcagagga aagctgctgg cagcgttgcg ccgacaaact cattgccaca ctgccaggaa
    13081 taaccaaagt tcgccgccct tttattgcgc tgatcctccc cgaaaaacca gaaatcgcca
    13141 atgagttggt aagccttgaa tgcccgcgga ctcattttca ttctaaggag tggttaaaag
    13201 ttgttgctaa tgaccccaca gcggtgagaa aactcgaacg ctactggagc caggatatat
    13261 ttagcgatcg agaagccagc tacatgtcgc atgaaaacca cttcggctac gcggcctgcg
    13321 ccgccctttt gcgcgaacaa ggactggcag ccgttccgcg cctcgcgata tatgcccata
    13381 aagaagattg cggcagtctg ctggtacaaa ttaaccatcc gcaagtcatc cgcaccttgc
    13441 tgttagttgc tgataaaaac aaacccagcc tgcaacgtgt ggctaaatac agtaaaaact
    13501 tcccccatgc gacactcgcc gcactggcag aactgctggc gttaaaagaa ccaccagccc
    13561 gcccaggtta tccaatcatc gaagacaaaa agctgcctgc acagcaaaaa gcacgcgatg
    13621 aatactggcg tacgctgtta caaacgctga tagcatcgca gccacaacta gcagaaaggg
    13681 tgatgccgtg gttaagtact caagccaggg cagtgctgaa tagttattta tcggcaccgc
    13741 caaaaccggt tattgatagc accgataaca gcagtctgcc tgaaatgctc gtttcaccac
    13801 cgtggcgtag taagaaaaaa atgacagctc cacgtcttga tttggcaccg ctcgaattaa
    13861 ctccgcaaat ttactggcaa ccaggcgaac aagagaggct tgccgccact gagtctgccc
    13921 gttatttcag cacggaatct cttgcggaac gcatggaaca aaaaagtgga cgagttgtat
    13981 tacaggaact gggttttggg gatgatgtat ggctgtttct gaattatata ctccccggaa
    14041 aactggatgc tgcacgcaat tcactcattg ttcagtggca ttactaccag gggcgggttg
    14101 aagagatcct gaatggctgg aactccccgc aaacacaatt agcagaacag gcgctccgca
    14161 gcggtcacat agaagcgtta attaacatat gggaaaatga caacttctca cgttatcgtc
    14221 cggaaaagag tgtctggaac ctgtatttat tggcacagtt gccgcgtgag atggctttga
    14281 ccttctggct gcgtatcatt gagaaaaagc atctgttcgc gggtgaggac tattttctca
    14341 gtatcctcgg attggatgcg ctaccaggtc tgctgttggc tttttcacat cgtccaaaag
    14401 aaacatttcc gttaatttta aattttggcg caacagaact ggcgctgcct gttgcccgcg
    14461 tctggcgacg tttcgcggcg cagcgtgatc tgg
//
LOCUS       DAEVZA010000124        14471 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEVZA010000124 DAEVZA010000000
VERSION     DAEVZA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.124"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..2099)
                     /gene="plsB"
                     /locus_tag="LAF92_003876"
     CDS             complement(<1..2099)
                     /gene="plsB"
                     /locus_tag="LAF92_003876"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBI9792806.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLA"
     gene            2270..2638
                     /gene="dgkA"
                     /locus_tag="LAF92_003877"
     CDS             2270..2638
                     /gene="dgkA"
                     /locus_tag="LAF92_003877"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBI9792807.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            2748..3356
                     /gene="lexA"
                     /locus_tag="LAF92_003878"
     CDS             2748..3356
                     /gene="lexA"
                     /locus_tag="LAF92_003878"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBI9792808.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            3429..4754
                     /gene="dinF"
                     /locus_tag="LAF92_003879"
     CDS             3429..4754
                     /gene="dinF"
                     /locus_tag="LAF92_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBI9792809.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            4870..5079
                     /locus_tag="LAF92_003880"
     CDS             4870..5079
                     /locus_tag="LAF92_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBI9792810.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(5121..5636)
                     /gene="zur"
                     /locus_tag="LAF92_003881"
     CDS             complement(5121..5636)
                     /gene="zur"
                     /locus_tag="LAF92_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBI9792811.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            5954..6970
                     /locus_tag="LAF92_003882"
     CDS             5954..6970
                     /locus_tag="LAF92_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HBI9792812.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNKDANKDKLDNIDATKDKLDNR
                     GTIKGKLNNVYGKSIDYSALRHRDIIIAKIDLFIQRITHNLWHARKKNVFLIEQITNL
                     KVWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNKDATPIERCTFDVE
                     TKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDDNSLKLFASLLIS
                     LSYSSAYTDLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKRLADGGEMNFDGYL
                     DKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGV
                     KSEK"
     gene            7333..8370
                     /gene="dusA"
                     /locus_tag="LAF92_003883"
     CDS             7333..8370
                     /gene="dusA"
                     /locus_tag="LAF92_003883"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBI9792813.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            8504..8746
                     /gene="pspG"
                     /locus_tag="LAF92_003884"
     CDS             8504..8746
                     /gene="pspG"
                     /locus_tag="LAF92_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBI9792814.1"
                     /translation="MLELLFVFGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(8912..9895)
                     /locus_tag="LAF92_003885"
     CDS             complement(8912..9895)
                     /locus_tag="LAF92_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBI9792815.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMISFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            9978..11393
                     /gene="dnaB"
                     /locus_tag="LAF92_003886"
     CDS             9978..11393
                     /gene="dnaB"
                     /locus_tag="LAF92_003886"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBI9792816.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            11446..12525
                     /gene="alr"
                     /locus_tag="LAF92_003887"
     CDS             11446..12525
                     /gene="alr"
                     /locus_tag="LAF92_003887"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBI9792817.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            12778..13971
                     /gene="tyrB"
                     /locus_tag="LAF92_003888"
     CDS             12778..13971
                     /gene="tyrB"
                     /locus_tag="LAF92_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBI9792818.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(14194..>14471)
                     /locus_tag="LAF92_003889"
     CDS             complement(14194..>14471)
                     /locus_tag="LAF92_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792819.1"
                     /translation="KIRGKKDFMYICTLCCFAEEVKGDYSHYRTFLFDIGNQYKVKLT
                     QSGKKELSLTLEFNDTIIESQKVTGNKAKHILEDIEIFYRNEPDTYY"
BASE COUNT         3509 a         3435 c         3781 g         3746 t
ORIGIN      
        1 gccagcagct gtagggtgcg agaatgcgcc gggttgatat gcaactcatc atcttgcagg
       61 gtaatcagcc cctgacgttg catctcattt gccagcgcat caataacgtc cggcaactcg
      121 tcgcgatccc agcgcaggaa cagctccgct ttcagcattg ggtaaagcac attgacgtgc
      181 tccatcaata cgtcgcggga gatgtggcga tgctgggtga caattgccgc catcagcgaa
      241 ggcagcacca acatatgcgc aatgttgttg cgatagtagg tcatcagcac cgcttgctcg
      301 cgtggcagaa tgatgatgtc gccgatagtg tctttctcga cttcaaactt gttcatttgc
      361 agcgcgtgat cgataagctc gctggcgctg gctgaaggaa cggtagagtc cgtggagtaa
      421 ggtacgttgc gcatcagatc caggtagcag ttgagttgct cggttaactg ctcgcgggtg
      481 agtgagcgct gacgtgatgc cagtagcgca gtacagcaca ggttcatggc gtttgccgcg
      541 cctgcgttgt taatgcgtac catcagatcg gcagcaatat tattgaccgt cggcgttaac
      601 caggccggac gcaccgcttc gatgggatcg atagattcac gccagtctgg tacgtgctgg
      661 ttaaggtagg tcatcaacgg cattggttca ccgaagttga cgtaaccctg accgagatta
      721 cgcagcttgc ttaaaccgcg cagcatctgc ggcaggctct ctttctcttt cgtcgcgccg
      781 cgcagttctt tggcgtaagt acccacttcc atgacgtgct cataaccgat atagatcgga
      841 atcagcgtaa tcggacgcgt gccgccacgc agcatcgcct gaatggtcat cgacagcgta
      901 ccggttttcg gatccagcaa acgccccgtg cgggaacgac cgccttccac gaagtactcg
      961 acggaataac cacggctgaa cagttcgccg agatactcac ggaaaacggt ggaataaagt
     1021 ttattgcctt taaaagtacg gcgaataaag aacgccccca gacggcggaa aatcggcccg
     1081 gccggccaga agttcaggtt gatcccggcg gcgatatgcg gcggcaccag cccctggtgg
     1141 tacagcacgt aagaaagtag caggtagtcc atgtgactgc ggtggcaagg cacatacacc
     1201 agctcatgac cgtcgtgggc cagctggcga acgcgctcgg cgttatggac gttgatgccc
     1261 tgataaagtc ggttccaggt gaagcccaga atgcggtcag tcaggcggat catctcgtaa
     1321 gagaaattcg ccgcaatctc ttccatcagc gcaatcgcgt tctgctgcgc tttttcatgg
     1381 gagatttttt tgctgcgcgc ttcatcttct accgctttgg caatggcgcg ggaggcgagc
     1441 agcttattaa acagatcctg acgagcaggc agacgtgggc ccacagcagc cagacgttga
     1501 cgggcaaagt gcatacgcgc cacgcgcgcc agtttctgtg cgatagtttt atccgtgccg
     1561 tgttcatccg ccatacggcg cagcgaaact gacggcgaga aacgcacaaa actgtcgcga
     1621 ccgagccaca gtacggcgaa aaatttctgt acgccgttaa gcatacgcag cggcgggttt
     1681 acttcgcctt tttcacgccc cggcgcgcga ccaaacatca ccgatactgg caccatctgc
     1741 acatccagat ttgggttgct acggtgcaaa tcgagatagt cgtggaacag cttaatagac
     1801 tcttctttcg gcgtgtaata ggtgaataca cgcggcccgc cgtgaatgaa cacatagcgc
     1861 ggcagtagcg tgccgtcgat ttccagcggc tctaacgggt caggcaagtc atgagccaga
     1921 cactgggcgc gtaacgtcag caaatctgct ttcgagttgt acggtaaaac gtacataatt
     1981 ggacgagaag tatccagccc cagttccggg gcaggatctg ccggaataga cttgcttttt
     2041 accaggatgc ttaatggtaa attcagtaat ttgtagtaaa ttcgtggcca gccggacata
     2101 aacgatgtaa agcctctggt taataatgca aatgcgcggc aaggatagca gaaagtcatg
     2161 ggaaattctg tggtatccgc tcatgtttcg cgcggcgtta cgcaaacccg aatcatcgga
     2221 tttaacggta cactgatatt gacgctcata atgtaaaaag gttctttcaa tggccaataa
     2281 taccactgga ttcacccgaa ttattaaagc tgctggctat tcctggaaag gtttacgcgc
     2341 tgcatggatc aacgaagcgg cattccgtca ggaaggcgta gcggtattgt tggcggtggt
     2401 catcgcctgc tggctggatg tggacgcaat tacccgcgtg ctgcttatca gctccgtgat
     2461 gctggtgatg attgtggaaa tcctcaatag cgccatcgaa gcagtggttg accgaattgg
     2521 ctctgaatac catgagcttt ccggacgcgc aaaagatatg ggatccgccg cggtgctgat
     2581 tgccattatc gtcgccgtga ttacctggtg cattctgtta tggtcgcatt ttggataacc
     2641 cttccagatt tcgataaatc tctggtttat tgtgcagttt atggttccaa aatcgccttt
     2701 tgctgtatat actcacagca taactgtata tacacccagg gggcggaatg aaagcgttaa
     2761 cggccaggca acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc
     2821 cgccgacgcg tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag
     2881 aacatctgaa ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg
     2941 ggattcgtct gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg
     3001 gtgaaccgct tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttgt
     3061 tcaagccgaa tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca
     3121 ttatggatgg cgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg
     3181 ttgtcgcacg tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag
     3241 tcgaactgtt gccagaaaat agcgagttta aaccaattgt cgttgacctt cgtcagcaga
     3301 gcttcaccat tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctgtaacata
     3361 tctctgagac cgcgatgccg ccaggcgtcg cggtttgttt ttcatctctc ttcatcaggc
     3421 ttgtctgcat ggcattcctc acttcatctg ataaagcact ctggcatctc gccttaccca
     3481 tgattttctc caatatcacc gttccgttgc tgggactggt cgatacggcg gtaattggtc
     3541 atcttgatag cccggtttat ttgggcggcg tggcggttgg tgcaacggcg accagctttc
     3601 tctttatgct gttgctgttt ttacgcatga gcaccaccgg gctgactgcg caggcttatg
     3661 gtgccaaaaa tcctcaggca ttagcccgtg cgctggtgca accgttgctg ttggcgttgg
     3721 gggctggggc gttaattgcg ctgctgcgta caccgattat cgatctggcg ctgcatattg
     3781 ttggcggtag tgaagcggtc ctggaacagg cgcggcgctt tcttgaaatc cgctggttaa
     3841 gcgctccggc gtcgctggcg aatctggtat tactcggttg gttactcggc gtgcaatatg
     3901 cccgtgcgcc agtaattttg ttagtggtcg gcaatatcct caacattgtg ctggatgtct
     3961 ggctggtgat ggggctgcat atgaacgtgc agggcgcggc gctggcgacg gttattgcgg
     4021 aatatgcaac attgctgatt ggtctgctaa tggtgcgtaa aatcctcaaa ctacgcggaa
     4081 tttccggcga aatgctgaaa actgcctggc gaggaaactt ccgtcgcttg ctggcgctta
     4141 accgcgatat catgctgcgt tcgctgttgt tgcaactctg tttcggcgcg atcaccgtac
     4201 ttggtgcgcg actggggagt gacattatcg ctgttaacgc ggttctgatg acgctactca
     4261 cctttaccgc ctatgcgctt gatggttttg cctacgcggt tgaagcgcac tccggtcagg
     4321 catacggtgc gcgcgacggt agccagttgc tggatgtatg gcgggcagcg tgccgccagt
     4381 cggggattgt agcgttactg ttttcggtgg tttatttgct ggcaggggaa cacatcattg
     4441 cgttgctgac gtcgttaacc cagattcagc agctggctga ccgctatctt atctggcagg
     4501 tgattttgcc gttggttggc gtctggtgtt atctgctgga cggcatgttt ataggcgcaa
     4561 cgcgtgccgc cgaaatgcgt aacagtatgg cggtggccgc cgcaggtttt gcgctgacgc
     4621 tccttacgct gccgtggctg ggtaatcatg gtttgtggct ggcattaacc gtctttctgg
     4681 cgttgcgcgg gctttctctg gcggctatct ggcggcgtca ctggcgcaac ggtacctggt
     4741 ttgccgcaac gtgacggtta aaaattctga ataaattatc ctaagccaaa ttgctgacta
     4801 cacttaatct cacgttcaga agaaaagtga acgtactctc attcacaacc taacgatgag
     4861 gtcttgatta tgaataaaga tgaagccggc ggtaactgga aacagtttaa aggtaaagtg
     4921 aaagagcaat ggggcaaact gaccgatgat gatatgacga tcattgaagg taaacgtgat
     4981 caactggtcg gtaaaatcca ggaacgttat ggttatcaga aagatcaggc agaaaaagag
     5041 gtcgtggatt gggaaacccg caatgaatat cgctggtaat taatccctcc tgcccgacgt
     5101 gtacaaggat gtacgccctc ttaacgcggt ttctttttca cctgcacaga gtgatcatgc
     5161 tggcactgtt caggatgacg acacgcttcc acttccacgc atgccgcaca gagcccatgt
     5221 gcttcaatca cattatgccg cagggcaaac cccatttttg ccgccagcgt atgcataatg
     5281 tcttccacgc cttctgcaca ctcttctttc accgcgccgc agcgatcgca aataaacatg
     5341 gctgacgtat gcgtgggctg gtcgaacaga tgacagagca cataactgtt ggtggattcc
     5401 accttatgca caaaaccttg ctcaagcaga aaatccagcg cgcgataaac cgttggcggc
     5461 ttggcttgcg gttcagcttc gcgcagcaaa tcaagcagat cataagcgct gatagcgcca
     5521 tcctgcagac tcatcaggcg caacacttcc aggcgctgtg gggtcaggcg cacattacgc
     5581 tgcgcgcaga ttttttcagc ctgcgctaat aactcctgcg ttgtggtctt ttccatcggg
     5641 cacctcaaag tgggggttaa gaatgtcctt actttaccat gttccaggaa aaacaccgag
     5701 aacccttatt gttgccgtaa tgttgatttt ctgttttgta cgtaaggtgt tgtgttgctt
     5761 ggtcgtacca ttatcaacac gataataatt aataattcat ttttaaataa attgcttcaa
     5821 gttaactcgc tatttattta actgggtttg atcgcatacc tgtagttcag aaattatgag
     5881 cgatttttga tagtgaccag atggcaaaac acttataagg aggacacggt acgacaaaaa
     5941 aggtagatga atcatgttga agataatacc cggtgcaact ggatacttta ataaaacact
     6001 caattcaaat caattcgata ataaagatgc aaacaaagat aaattagata atatagatgc
     6061 aaccaaagat aaattagata atagaggtac aatcaaaggt aaattaaata atgtatatgg
     6121 taagtctatt gattattcag cgctgcgtca tcgggatata attattgcaa aaatagattt
     6181 gtttattcaa agaatcactc ataatttatg gcatgcacgt aaaaaaaatg tgtttttaat
     6241 tgagcaaata actaatctca aagtgtgggt caataagtat attgacgatt gcactgatga
     6301 ggatttaaac gatcgtgact ttattgcatc agttgttgac cgggctattt ttcatttcgc
     6361 gattaatagt atatgtaatc ctggggataa taaagatgcg actcccattg aacgatgtac
     6421 ttttgatgta gaaactaaga atggccttcc ctccactgtt cagctatttt atgaggaatc
     6481 taaggataat gaacctttag cgaatataca ttttcaagca ataggttctg gttttttaac
     6541 gtttgttaat gcctgccagg aacatgatga caacagctta aaattatttg cttcgctgtt
     6601 aatttcactt tcatattcta gtgcctacac agatttatca gaaacagtgt atattaatga
     6661 aaataatgag agctacctga aagctcagtt tgaaaaatta tctcaacgtg atatgaagaa
     6721 gtacctggga gagatgaagc gtctggctga tgggggagaa atgaattttg atggctatct
     6781 ggataagatg tcacatctgg tgaatgaagg aacgctcgat cctgatattt taagcaaaat
     6841 gcgagatgct gcaccacaat taattagctt cgcgaagtcg tttgacccaa cctcaaagga
     6901 agagattaaa atacttacag acacttctaa attaatttat gatttgttcg gggttaaatc
     6961 ggagaaataa tatgtgaagt tcttcgatag tatggaaggc attatataaa aggacccaat
     7021 atttattggg ttcttttttc tctatcaatg ctattagcag ggagatatat caccagagtt
     7081 taatgtgtga ttttttattt atcgtcgaac ctggattgtt tatcattggc cttaacaaag
     7141 ttaacggcta ataagattat ttccatcact tcgtgagagc ttcatgcctt gagaggatct
     7201 caattttctt ttgcaatgag acaggcgctt cctgttgtta tggtatagta ccccgctatt
     7261 gagcctcctg aatagtgatg ctgaataaca taaccccatg atatatcgat aaaataatct
     7321 ctacatttga aaatgcacgg taattctgaa atgcaaaaaa tcaaccaaac cagcgcaatg
     7381 cctgaaaaaa ctgacgttca ctggagtggt cggtttagcg ttgcaccaat gctcgactgg
     7441 acggacagac attgccgcta tttcttgcgt ctgctttccc gcaatacgtt gctgtatacc
     7501 gaaatggtga ccacaggggc gattattcac ggtaaaggtg attacctggc gtacagtgaa
     7561 gaagaacatc cggtagcgtt gcaactcggc ggtagcgatc cggcggcgct ggcacagtgt
     7621 gcgaagctgg cagaagcgcg tggatatgat gagatcaacc tgaatgtcgg ctgcccgtct
     7681 gaccgggtgc agaacggcat gtttggtgcg tgtctgatgg gtaatgcgca gctggttgcc
     7741 gactgcgtga aagcgatgcg cgatgtggtg tcgattccag tgacggtgaa aacgcgtatt
     7801 ggcatcgatg accaggacag ctatgaattt ctctgcgatt tcatcaacac cgtttccggc
     7861 aaaggcgagt gtgagatgtt tatcatccac gcacgtaaag cctggctttc ggggttaagc
     7921 ccgaaagaaa accgtgaaat cccgccgctc gattatccgc gtgtgtatca actgaagcgt
     7981 gactttccgc atctgacaat gtcgattaac ggtggtatca agtcgctgga agaggccaaa
     8041 gcacacctgc aacatatgga tggcgtgatg gtcgggcgcg aggcgtatca gaatccgggt
     8101 attctggcgg cggtagaccg ggagatcttt ggttcctcgg ataccgatgc cgatccagtg
     8161 gcggtagtgc gcgccatgta tccgtacatt gagcgtgaac tcagccaggg gacgtatctc
     8221 ggccatatta cccggcatat gttgggcttg ttccagggta ttcctggcgc gcggcagtgg
     8281 cggcgttatt taagtgaaaa tgcccataaa gcgggtgcag acattaatgt gctggaacac
     8341 gcgctcaaac tggtggcgga taagcgttaa cttttcacca aaaagtagtc aaattcacca
     8401 cgccctgcgc accgtcgcgg ggcattttgc tgttaaatca atagattatt tttggcatga
     8461 ttcttgtaat gccagcaaga gatttcatat ttgggagagc atcatgctgg aactactttt
     8521 tgtgtttggc ttttttgtca tgctgatggt caccggcgtt tcgttgctgg gcattatcgc
     8581 cgcgctggtt gtggcgacgg ccattatgtt cctcggcggt atgctggcat tgatgattaa
     8641 gttgctgccg tggttactac tggcgattgc ggtggtgtgg gttattaagg cgattaaagc
     8701 accaaaagtg ccgaaatatc agcgttatga ccgctggcgt tactaaggga ttgtgcggat
     8761 gatcacaacc taaggtttta tccttagaac aaaataggaa ttgataatca aatctgtcac
     8821 tattgcgcct ctaacagatt catcgtgctg taccctacat acagccgaac tataaaaaga
     8881 aagggcttcc caggtggaag ccctatttct tttatggaat cagcaggctg gaaccttgcg
     8941 tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc cttcagcgga tatttctgct
     9001 gctcggcgac atcgacctta atcacaccgc tggcaatcaa agagaacagt tcattactgg
     9061 cctcggttaa ttcctcccgc gtggtgatat agccttgcag ggaagggcgt gtcacataca
     9121 acgagccttt ttgattgaga atgcctaagt tcacaccggt aaccgcacct gatgagttgc
     9181 caaaactgat cattaagccg cggcgttgca ggcaatccag cgaccgttcc caggtgtctc
     9241 tgcccacgga atcgtacact acgcgcactt tcttaccgcc ggtgatctct ttcaatcgct
     9301 cgaccagatt ctcttcacga tagttaataa cctgccacgc gccggctttt agcgcgctct
     9361 gcgctttttg cgcggttcct acggtgccga taagtttcgc gcccagggct tttgcccact
     9421 ggcaggcaat taagccaacg ccgccagccg ctgcgtggaa caggaactgt tcatcgggtt
     9481 taatttcata ggttttgcgc agcagataat aaaccgttaa gcctttcagg aaagatgctg
     9541 cagcttgttc aaaagaaatt gccgcaggca gaatcgccgc tttatccgca ttaatgttat
     9601 gcacagagct gtaagcgcct aacgccgact gcgcatagac tacacgatcg cctgccttaa
     9661 tatgctttac accactgccg actttactca cgatgcctgc cgcttcggta cctaatccgc
     9721 tgggtagtga tggcggcggg taaaggccgc tgcggatata cgtgtcgata aaattgatgc
     9781 cgatggcttt attttcgacc tggatttcat tctctgccgg atcggcagga gtgaactcta
     9841 cggcttgaag tacttccggg ccaccgtgct tgtgaaattc aattcgtgtt gccatgtgtc
     9901 ctccagaacg tatcgtcagg gtctgcttca tatggtaaag tttcgaccca ttctttatct
     9961 cggtaactcc attcactatg gcaggaaata aacccttcaa caaacagcag gctgaacccc
    10021 gcgaacgcga tccacaagtt gccgggctga aagtgcctcc gcactcgatc gaagcggagc
    10081 agtcggtgtt gggcggttta atgctggata acgaacgctg ggacgatgta gccgagcgtg
    10141 tggtggcaga cgatttttac acccgcccac accgccatat ctttactgaa atggcgcgtt
    10201 tgcaggaaag cggtagtcct atcgatctga ttacccttgc ggaatcgctg gaacgccagg
    10261 ggcaactcga tagcgtcggt ggttttgctt atctggcaga gctgtcaaaa aatacgccaa
    10321 gtgcggcgaa catcagtgcc tatgcggaca tcgtgcgtga acgtgccgtt gtccgtgaga
    10381 tgatctcggt tgcgaatgag attgctgaag ccggttttga tccgcagggg cgtaccagcg
    10441 aagatctgct ggaccttgct gaatcccgcg tctttaaaat tgccgaaagt cgtgcaaaca
    10501 aagacgaagg gccgaagaac atcgccgatg tgctcgacgc aaccgtggcg cgtattgagc
    10561 agttgtttca gcagccacac gatggcgtta ccggagtaaa caccggttat gacgatctca
    10621 acaaaaaaac cgctggcttg cagccgtcgg atttgatcat cgtcgccgcg cgtccgtcga
    10681 tgggtaaaac aacatttgcg atgaacctcg tcgaaaacgc ggcgatgttg caggataaac
    10741 cggtacttat cttctcgctg gagatgcctt cagaacagat tatgatgcgt tctctggcgt
    10801 cgctgtcgcg cgttgaccag actaaaatcc gtaccgggca gctcgatgat gaagactggg
    10861 cgcgcatttc cggcaccatg ggtattttgc tcgaaaaacg caatatctat atcgatgact
    10921 cttccggcct gacgccaacg gaagtgcgtt cccgcgcacg ccgtattgcc cgtgaacacg
    10981 gcggcatcgg gcttatcatg atcgactacc tgcaactgat gcgcgtaccg gcgctttccg
    11041 ataaccgtac gctggaaatt gcagaaatct cccgctcgct gaaagcactg gcgaaagaac
    11101 tgaacgtgcc ggtggtggcg ctgtcccagt tgaaccgttc tctggaacaa cgtgccgaca
    11161 aacgcccggt caactccgac ctgcgtgaat ctggctctat cgagcaggat gcggacttga
    11221 tcatgtttat ctatcgtgat gaggtgtatc acgaaaacag tgatttaaaa ggcatcgcgg
    11281 aaattattat cggtaaacaa cgtaacggcc caatcgggac ggtacgcctg acctttaacg
    11341 gtcaatggtc gcgcttcgac aactatgcgg ggccgcagta cgacgacgaa taataattat
    11401 tttatgaatt aggtaattaa agcaaacact tatcaaggaa cacaaatgca agcggcaact
    11461 gttgtgatta accgccgcgc tctgcgacac aacctgcaac gtcttcgtga actggcacct
    11521 gccagtaaaa tggttgcggt ggtgaaagcg aacgcttatg gtcacggtct tcttgagacc
    11581 gcgcgaacgc tccccgatgc tgacgccttt ggcgtagccc gtctcgaaga agctctgcga
    11641 ctgcgtgcgg ggggaatcac caaacctgta ctgttactcg aaggcttttt tgatgccaga
    11701 gatctgccga cgatttctgc gcaacatttt cataccgccg tgcataacga agaacagctg
    11761 gctgcgctgg aagaggctag cctggacgag ccggttaccg tctggatgaa actcgatacc
    11821 ggtatgcacc gtctgggcgt aaggccggaa caggctgagg cgttttatca tcgcctgacc
    11881 cagtgcaaaa acgttcgtca gccggtgaat atcgtcagcc attttgcgcg cgcggatgaa
    11941 ccaaaatgtg gcgcaaccga gaaacaactc gctatcttta ataccttttg cgaaggcaaa
    12001 cctggtcaac gttccattgc cgcatcgggt ggcattctgc tgtggccaca gtcgcatttt
    12061 gactgggtgc gcccgggcat cattctttat ggcgtctcgc cgctggaaga tcgctccacc
    12121 ggtgccgatt ttggctgtca gccagtgatg tcactaacct ccagcctgat tgccgtgcgt
    12181 gagcataaag ccggagaacc tgttggttat ggtggaacct gggtaagcga acgtgatacc
    12241 cgtcttggcg tagtcgcgat gggctatggc gatggttatc cgcgcgccgc gccgtccggt
    12301 acgccagtgc tggtgaacgg tcgcgaagta ccgattgtcg ggcgcgtggc gatggatatg
    12361 atctgcgtag acttaggtcc acaggcgcag gacaaagccg gggatccggt cattttatgg
    12421 ggcgaaggtt tgcccgtaga acgtatcgct gaaatgacga aagtaagcgc ttacgaactt
    12481 attacgcgcc tgacttcaag ggtcgcgatg aaatacgtgg attaatcgtt ctgtaatatt
    12541 tgattgtctg tgccggatgc ggcgtgaatg ccttatccgg ccaataaaat cctaaaaatt
    12601 caataagttg atgttctttc atgctcttat aaaggtcgtg cctctggcgg atgtacgttt
    12661 gtcatgagtc tcactctgtt gctaattgcc gttcgctcct gaacatccac tcgatcttcg
    12721 ccttcttccg gtttattgtg ttttaaccac ctgcccgtaa acctggagaa ccatcgcgtg
    12781 tttcaaaaag ttgacgccta cgctggcgac ccgattctta cgcttatgga gcgttttaaa
    12841 gaagaccctc gcagcgacaa agtgaattta agtatcggtc tgtactacaa cgaagacgga
    12901 attattccac aactgcaagc cgtggcggag gcggaagcgc gcctgaatgc gcagcctcat
    12961 ggcgcttcgc tttatttacc gatggaaggg cttaacagct atcgccatgc cattgcgccg
    13021 ctgctgtttg gtgcggacca tccagtactg caacaacagc gcgtagcaac cattcaaacc
    13081 cttggcggct caggggcatt gaaagtgggc gcggatttcc tgaaacgcta cttcccggaa
    13141 tcaggcgtct gggtcagcga tcctacctgg gaaaaccacg tagcaatatt cgccggggct
    13201 ggattcgaag tgagtactta cccctggtat gacgaagcga ctaacggcgt gcgctttaat
    13261 gacctgttgg cgacgctgaa aacattacct gcccgcagta ttgtgttgct gcatccatgt
    13321 tgccacaacc caacgggtgc cgatctcact aatgaccagt gggatgcggt gattgaaatt
    13381 ctcaaagccc gcgagcttat tccattcctc gatattgcct atcaaggatt tggtgccggt
    13441 atggaagagg atgcctacgc tattcgcgcc attgccagcg ctggattacc cgctctggtg
    13501 agcaattcgt tctcgaaaat tttctccctt tacggcgagc gcgtcggcgg actttctgtt
    13561 ctgtgtgaag atgccgaagc cgctggccgc gtactggggc aattgaaagc aacagttcgc
    13621 cgcaactact ccagcccgcc gaattttggt gcgcaggtgg tggctgcagt gctgaatgac
    13681 gaggcattga aagccagctg gctggcggaa gtagaagaga tgcgtactcg cattctggca
    13741 atgcgtcagg aactggtgaa ggtattaagc acagagatgc cagaacgcaa tttcgattat
    13801 ctgcttaatc agcgcggcat gttcagttat accggtttaa gtgccgctca ggttgaccga
    13861 ctacgtgaag aatttggtgt ctatctcatc gccagcggtc gcatgtgtgt cgccgggtta
    13921 aatacggcaa atgtacaacg tgtggcaaag gcgtttgctg cggtgatgta atgcaggaaa
    13981 gcaggctgga gctacccagc ctgcagtgaa attaaactgt cgtcgctttc actctttctt
    14041 aatagatgat ttttttgatg ccgttgttct acgtgagaga taataaaacg ttgtcagttc
    14101 ttttattgtt aagtttatcc caattatctg gaattccttt ccctattttt tcgtgtggag
    14161 taataatttc tgcacatcta accattatta aatttaataa taagtatcgg gttcgttacg
    14221 atagaatatt tctatatcct caagaatatg ctttgcctta ttacctgtga ctttctgaga
    14281 ttcaattata gtatcgttaa attctaatgt taaagagagc tctttttttc cgctttgtgt
    14341 aagtttaacc ttatattgat taccaatatc aaataaaaag gtcctgtaat gagaataatc
    14401 acctttaacc tcctcggcaa aacagcaaag cgtgcaaata tacataaagt ctttcttacc
    14461 cctgattttg t
//
LOCUS       DAEVZA010000125        14387 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEVZA010000125 DAEVZA010000000
VERSION     DAEVZA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.125"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..2066)
                     /gene="yccS"
                     /locus_tag="LAF92_003890"
     CDS             complement(<1..2066)
                     /gene="yccS"
                     /locus_tag="LAF92_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBI9792820.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVV"
     gene            complement(2076..2522)
                     /locus_tag="LAF92_003891"
     CDS             complement(2076..2522)
                     /locus_tag="LAF92_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBI9792821.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2645..4699
                     /gene="helD"
                     /locus_tag="LAF92_003892"
     CDS             2645..4699
                     /gene="helD"
                     /locus_tag="LAF92_003892"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBI9792822.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4731..5189)
                     /gene="mgsA"
                     /locus_tag="LAF92_003893"
     CDS             complement(4731..5189)
                     /gene="mgsA"
                     /locus_tag="LAF92_003893"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBI9792823.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5285..5947)
                     /locus_tag="LAF92_003894"
     CDS             complement(5285..5947)
                     /locus_tag="LAF92_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBI9792824.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6120..6533
                     /locus_tag="LAF92_003895"
     CDS             6120..6533
                     /locus_tag="LAF92_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBI9792825.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6578..6895)
                     /gene="hspQ"
                     /locus_tag="LAF92_003896"
     CDS             complement(6578..6895)
                     /gene="hspQ"
                     /locus_tag="LAF92_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBI9792826.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(6953..8143)
                     /gene="rlmI"
                     /locus_tag="LAF92_003897"
     CDS             complement(6953..8143)
                     /gene="rlmI"
                     /locus_tag="LAF92_003897"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBI9792827.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8238..8516
                     /gene="yccX"
                     /locus_tag="LAF92_003898"
     CDS             8238..8516
                     /gene="yccX"
                     /locus_tag="LAF92_003898"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBI9792828.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTIQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            complement(8513..8842)
                     /gene="tusE"
                     /locus_tag="LAF92_003899"
     CDS             complement(8513..8842)
                     /gene="tusE"
                     /locus_tag="LAF92_003899"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBI9792829.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8933..9592)
                     /gene="yccA"
                     /locus_tag="LAF92_003900"
     CDS             complement(8933..9592)
                     /gene="yccA"
                     /locus_tag="LAF92_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBI9792830.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9799..9886)
                     /locus_tag="LAF92_003901"
     tRNA            complement(9799..9886)
                     /locus_tag="LAF92_003901"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9850..9852),aa:Ser,seq:tga)
     gene            10313..11431
                     /gene="hyaA"
                     /locus_tag="LAF92_003902"
     CDS             10313..11431
                     /gene="hyaA"
                     /locus_tag="LAF92_003902"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBI9792831.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11428..13221
                     /gene="hyaB"
                     /locus_tag="LAF92_003903"
     CDS             11428..13221
                     /gene="hyaB"
                     /locus_tag="LAF92_003903"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBI9792832.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13240..13947
                     /gene="hyaC"
                     /locus_tag="LAF92_003904"
     CDS             13240..13947
                     /gene="hyaC"
                     /locus_tag="LAF92_003904"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBI9792833.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            13944..>14387
                     /locus_tag="LAF92_003905"
     CDS             13944..>14387
                     /locus_tag="LAF92_003905"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBI9792834.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARDQLPAAEQS"
BASE COUNT         3592 a         3689 c         3840 g         3266 t
ORIGIN      
        1 acgaccagag gttctttgct gtctgcccgt ggttcaagtt gctgcatccg ctgtttcagg
       61 ctagctaatg cctcattgac gcgttcttca tcagcaggtt gatgatgtaa cgcgtcatca
      121 acatagcaaa ctgcgtcatc aagaaacgcc agaatttcag gattagttaa ctgctcccgg
      181 tgagcaccga gggctgagat atagctggta aacgtatggt taaggcacag caaccgaaac
      241 gcggcttcgc gaatttgcgg ggtaacgttc ggctcgctgg acatatttga taccaccgac
      301 gccagctcag catcacggtt gtgtgcatcg cggcgggcaa tacgatacgc cagacggtta
      361 tcacgcccct gatggtattg ctccagtatg gcatcgagat accgacagtt ggcctctgtg
      421 gcgcgttcga gcatgcgcgg cagattgcga aactgccagt caggccagat gtagctcact
      481 gccgcccacg caatggcaca accaatcagc gtatcgatta cgcgaggtaa cgctacttca
      541 aaaccttcac ccagtaagtt aaaacacagt agcaccaaaa gtgtgatgaa catcgttgca
      601 tgagcgtatt gcacgttacg gaaggcaaaa aagagcacgc cggtaataac cagcagcacc
      661 agctgccctt ccagtgatgg cacaaaccac agcacaggaa tgccaatggc gatacctacc
      721 agcgtaccaa taatccttaa cttcaggcgg tggcgcgtgg cgttatagtt tggctggcag
      781 acaaacaaac ttgtcagcaa gatccaatac ccgtgatgca ttccggttat ttgaatgatg
      841 gcgtagccga agcacaacac cagcgacatt cttaccgcat gacggaagag ggcggattcc
      901 ggcgtgaagt gacggctaag acgcagccag atatcactca acccgtgcgg gctgtcatca
      961 gcgagctcat tttcgtcatt attatggggt agtgcctggg cctgttctga ttcaattgtt
     1021 gccagttggg catcaatggc gcgtaaattg ttcagcaaaa atcccagtgt tttgagtaaa
     1081 tcggcgggtg cgccgttatc gcgcatccgc tccagcgcag catcaatatg cgtaaaagcg
     1141 cgctcaaaat gcggatcatg ttgataaggc tgacgcagca aaatacagcg tgacagttgc
     1201 tggcacgcct ggccctgcat cgacatcagc cgctgaaaac ggaacagcac gtcgctgtgg
     1261 cgaaaatgtt cacgcaatgt ttgatactga atatgagaag agctggcacg ctcgtgaata
     1321 tcctgcgcga caaagtaata atgcagcgtg cgacgcgttc cccgttgacc acgatcgcca
     1381 cgtaagcggg tcagcagcga gagtttcgtc tgattcaatg tcgccatcag ttgtccgttg
     1441 gcgagagcca aatcgtacag cggtgcctgg ctttcatctt caatatcagg atcaaacatg
     1501 cgcgacttga gctcaagata acgcgccagt tgttcatagc aacgcgccag gttgtcctgc
     1561 agcgggcgga ccgggaacag cagatgacca ataagtgtca ggacgttgta ccagacggca
     1621 ccggccagca gatacatcgg ctgctgatac cagtgctcat acagtgatgt tcccaacata
     1681 gtgtaaatgg cgatcagcaa tgcaccgaag gcaattgttg catagcgttg acccagaccg
     1741 ccgagcaaaa tgaagccgct ggtagagagc gttaagccaa tcgcaaatag ccagggccag
     1801 ggaaacagca attctactga ggccgaggcg ataaaaaagc agaacagcgt aatgatgagg
     1861 ttacgcaaac gtcccgccag tcggtcatcg agatcggtca gcgccgctgc caccatcccc
     1921 agcgttagcg gaatcgtcag ttttacatca cccagccacc acggaaacgc tgtggttcca
     1981 caaagcgcaa taaaaatacg cgcgtaatac agccaggcgc tgttccaggt atagcgtttg
     2041 agcaaaggac ttagcataaa ggccatatta gtggattatt caaaacgacg acgtgcattg
     2101 gcttcacgcg cagcttgcgc tgtttctacc gataccacgc gacgaccaac cggccatagt
     2161 gcaatagcgg caattttaaa gttcgcaatg ccgacaggaa tgccaatgat tgaaatacat
     2221 tgtgcgatgc ccgttgcaat gtgcatcagg cataaccacc agccaaagaa aatcagccag
     2281 aaaatattca ataccgtacc gccagtattc agcagcacat ttttgccagc cgggttcagt
     2341 tcatcgacat gaatagcttc attgccataa ggcaccagag acagtttagt gatctcccag
     2401 caggatcgtg tcagcggtaa ggtaaaaatc agcacaatac tgaccagagt cgccaacagc
     2461 cagcccagag tggtggcaaa tccgccaagc acaaagttca gaatgttcaa aacggtacgc
     2521 ataaaacctc gctttactgt ggttttcagt aatgggcggc aattgtaacg tttttttggg
     2581 ctggagcacg ttttctctga cggttacact gataagaaat aatttcgtgt ggatctacag
     2641 agtcatggaa ctgaaagcga caacgcttgg aaaacgtctg gcacagcacc cttacgatcg
     2701 ggcggtgatc ctcaatgccg ggattaaagt ctccggcgat cgccacgaat accttattcc
     2761 tttcaatcaa ttactggcga ttcactgtaa gcgcggtctg gtatggggcg agctggaatt
     2821 tgtactgccg gacgaaaaag tggtgcgtct gcacggcacc gaatggggcg agacgcagcg
     2881 tttttaccat catcttgatg ctcactggcg gcggtggagt ggcgagatga gcgaaattgc
     2941 gtctggtgtt ttacgccagc aactggattt gattgccacg cgcactggag aaaataaatg
     3001 gctgacgcgt gagcaaacct ctggcgttca gcaacaaatc cgccaggctt tgtcggcgtt
     3061 gccgttgccg gttaaccgac tggaagaatt cgataactgc cgtgaggcgt ggcgtaaatg
     3121 tcaggcctgg ttgaaagata ttgaaagcgc tcggttgcag cataaccagg cgtataccga
     3181 agccatgctt accgagtatg cggatttttt ccgccaggtc gagtcttcac cgctgaatcc
     3241 ggcgcaggcc cgggcagtcg ttaatggcga gcattctctg ttagtgctgg caggtgcagg
     3301 aagcggaaaa acgtcggtgc tggtggcccg tgcaggctgg ttgctggcgc gtggtgaagc
     3361 gtcccctgag caaattttat tgctggcgtt tggtcgcaaa gccgctgaag agatggacga
     3421 gcgtattcgc gaacggctac ataccgaaga cattaccgca cgcacgtttc atgcgctggc
     3481 gctgcatatt attcagcagg gcagcaaaaa agttccgata gtcagcaaac tggaaaatga
     3541 taccgctgcc cgtcatgaac tctttattgc tgagtggcgc aagcaatgca gcgaaaagaa
     3601 agcgcaggcg aagggctggc ggcaatggct gacggaagaa atgcagtggt cagtgccaga
     3661 aggtaacttc tgggatgatg aaaaattaca gcgtcgtctt gcctcacgcc tcgatcgttg
     3721 ggtaagtctg atgcggatgc acggtggtgc acaggcagaa atgattgcca gtgcacccga
     3781 agagattcgc gatctgttca gtaaacgtat caagttgatg gccccgttat taaaagcctg
     3841 gaaaggtgcg ctgaaggcag aaaacgctgt cgatttttcg ggccttattc atcaggcgat
     3901 tgtgattctg gagaaaggtc gctttatcag cccgtggaag catattctgg ttgatgaatt
     3961 tcaggatatc tcgccgcagc gggcagcgtt gttagcggca ttacgcaagc aaaacagtca
     4021 gacgacgttg ttcgccgttg gtgatgactg gcaggcgatt taccgattca gcggtgcgca
     4081 aatgtcgctc accaccgctt tccatgaaaa ctttggtgaa ggcgatcgct gtgatttaga
     4141 cacgacttac cgttttaaca gtcgtatcgg tgaggtggca aaccggttta ttcagcagaa
     4201 cccaggccag ttgaaaaagc cgctaaacag cttaaccaat ggagacgaaa aagccgtcac
     4261 gttattggat gagagtcaac ttgacgcttt gctggataag ctctctggtt atgccaaacc
     4321 ggaagagcgc attcttatcc tggcgcgtta ccatcacatg aggcctgcca gcctggaaaa
     4381 agcggcaaca cgctggccga agttgcaaat cgactttatg accattcatg ccagcaaagg
     4441 gcaacaggcg gattacgtca tcatcgttgg cttgcaggag ggaagtgatg gttttccggc
     4501 tgcggcgcgg gagtcgatta tggaagaggc gctactgcca ccggttgagg atttcccgga
     4561 cgctgaagaa cggcggttaa tgtacgtggc gctgacccgg gcacgccatc gggtatgggc
     4621 actgtttaac aaagagaatc cctctccctt tgtggaaata ctgaaaaatc tggatgtgcc
     4681 ggtggcgaga aaaccgtaag aaacaggtgg cgtttgccac ctgtgcaata ttacttcaga
     4741 cggtccgcga gataacgctg ataatcgggg atcagaatat cgaccgcgtc gttgaaatgc
     4801 ggcgactgga ttatgaagtc tgccgttgcc acgttggtgg cgaccggaat gttccatacc
     4861 gtcgccagac gcagcaaggc tttcacgtca ggatcgtgcg gcacggcatt tagtggatcc
     4921 cagaagaaaa tcaatacatc aattttccct tctgagatca atgcgccaac ctgctggtca
     4981 ccccccattg ggccactcaa catcgcgttg acgttcatgc cggtcgcgcg ggaaattaag
     5041 ttaccggtag tgcctgttgc atacagtacg tgttgttcca gtaacggttg atgccgttcc
     5101 acccagctca tcagcatttg tttgcagtga tcgtgtgcca ccagcgcaat atgtttccgc
     5161 gcaggtaaag tgcgagtcgt cagttccata atgtacatcc gtagttaact ttcctacaga
     5221 ttactgtaag cacttatcgc tgcaagataa agaccgaaaa agcctgcgca caggcacaaa
     5281 aatctcagga agatggttgt ttttccgccc actgcaggaa agtatttcgc gtttgtgggt
     5341 cagccagttt aaaccaatac ttcagccgtt gttctgtgag cacctgagac tgcggtggaa
     5401 tagcatccag ctcggaaaca cccgagagca gcgtactgtc atctgccatc atcgtggcaa
     5461 attgcggtag tgaagcgcgt ttcgcggatt tgttatagcg ttcaacctcg acctcataat
     5521 caatcgtttt tgccgttgag gtaatggcga gaatatccag ctttaccgga atcggcgtcg
     5581 catcgccatc caacaattca aggcgcggcg cggcatcaaa atggttagct tcccgctcat
     5641 tctccaggcg aggcaggcga aaattgacct ggttgatgag ctgggtatta aaactgacca
     5701 ccagcggtgg ggagatatac agccgttctt cactattgga aagatgaatc gtcttctcaa
     5761 cgcgaaacac taactgatgc ggtccattat ccagttcaat gctgtccgca ccgcgcagca
     5821 gagagctgga aaccttcttg ccgtcgagta ccagaagatc gacatcggtt gaaagccgca
     5881 gcgtggtggc aaaaacggat accggcaaac ataatgcaat caaggtggtc acgatgccgg
     5941 ttttcatagg tagctcctgt caaaagaccg cccgaataat aatgtgtcaa aatttttctt
     6001 taatcatact tactaacata tagacatatt tcccgctttt gctctcattc attcgtatta
     6061 gctgcatggt tggcatgtcg gctttgtcgt acactttgtt aaacagtctg gaggaaataa
     6121 tgaaagaaac cgatattgca ggcattttaa cgtccactca caccattgcg ctggtggggg
     6181 caagcgacaa acccgatcgt cccagctatc gcgtgatgaa atatctactc gaccaggggt
     6241 atcacgtcat tccggtttcg ccaaaagttg ccggcaaaac gctgctgggc cagcaggggt
     6301 atggcacgct ggcagacgtt ccggaaaagg tcgatatggt cgatgttttc cgtaattcag
     6361 aagcggcgtg gggcgtggca caggaagcta ttgccattgg cgcgaaaacc ctctggatgc
     6421 aactgggggt gattaatgag caagcggcag tactggcacg ggatgccggg ttaaatgtgg
     6481 tgatggatcg ctgcccggct attgagatcc ctcgcctggg cctggccaaa taaaaaatcc
     6541 ccggaaggca aaaaccttcc ggggattcgt tcagggatta gttacgcaga cgcggggcct
     6601 ggagttgttt gcggatggtc tgcgccagct catccatgga tggctgttca ggatgttcat
     6661 cctgcaattc gctacttagc tgggcttcag ccaggtaggt atgaaccggt aggccgttat
     6721 cgtcctccat caccacgtga taccacggag cggcgcgaag ctcgtcattc accgccagct
     6781 catcaggcga cggttcagag agcgaataaa ccgggtcgat atccacgacc actccgagat
     6841 aacctaacag ggaatggcgg acctgctggc cgataccgaa tttgctggca atcatagtca
     6901 cctcccggga aatctttacc tttgatataa gggtaaaatt ccacatttca agttacatga
     6961 cgcgacaggc aaaccctttc agatatagcc cttccgggta ggtagcgatc accggatgat
     7021 cggctgcctg acggaactgc tctataaatt gtacatcacg gccggcatca attgcggcat
     7081 ccgcgatgat tttctgaaat aaatcgctgg tcatcagacc ggaacaggag aaagtcagga
     7141 gaataccgcc ttcattcagc aactgaatcg ccagcatgtt gatatcttta tagccacgac
     7201 acgcgcccat caactggctt ttattctcaa caaacttcgg cgggtccatc acgataacgt
     7261 caaatttttc accgcgatcg cgataagtac gcagcaattt aaagacatca tcacggacaa
     7321 actcagcctt gctcagatcc agtttgttca gctcaacgtt ctgccgtgca atatccagcg
     7381 cttcctggga ggtatcaacg ctgacaacct ggctgcaacc gcccatcagt gccgatacgg
     7441 cgaaaccacc ggtataggag aaacagttca gcacacgttt attttcaacg tagcggcggg
     7501 tagccaggcg gctatcacgc tggtccaggt agtagcccgt tttgtgtccg tgctgaatat
     7561 ccaccagcag tttcatgccg tgttcttcaa tcggcagcag ggcaggtggc aactcgccgg
     7621 tgacgaggcc ctgggtcagc tccatccctt cttttttacg taccgcaaca tcgctgcgat
     7681 cgtaaatcgc acattccggg tacagcgttt gcagggcact aattaatgcc gcgcgctgat
     7741 attctgcgcc agcactcagc agttgcagca ccagaaaatt accgaaacga tcgatagtaa
     7801 tacccggcag gccatcagat tctccggcga ttaaacgata gctgtcgagg ccatcttttt
     7861 gcgccagcca gtcacgccat ttttgtgctt gttgcaaacg gcgggaaaaa aaagcaatgt
     7921 cgatagactc agacgggtca aacgtccaga cgcgcgcccg gatttgcgaa gctggtgaat
     7981 aagcgccgcg tgctaaccat tttccctgat gatcaacaat atcgatggtt tcaccgaggc
     8041 tggctttacc ttccatgcgg gcaacggccc cggaaaagac ccacggatgg cgacgaagta
     8101 atgatttttc gcgccctttg gctaacacta aacgtacact cataatttac ttttctgtcg
     8161 atgcaaagaa atgggcgtca ttgtccggag ttttccatgg atttgcaacg cgtccattaa
     8221 ggataaggag agcagcaatg tcgaaagtct gcataattgc ctgggtttac gggcgagttc
     8281 agggcgtagg atttcgctac accatacagt atgaagcgaa aagactgggt ttaaccgggt
     8341 acgccaaaaa tcttgacgat ggcagcgtgg aagtggttgc ctgcggtgaa gaagggcagg
     8401 tggaaaaatt aatgcagtgg ttaaagagtg gcggcccgcg ttctgcgcgt gtggaacggg
     8461 tgcttagcga gccgcatcat ccctcggggg aattagccga ttttcgtatc cgttaaatac
     8521 attttaccgg ttttggcagg ccagcaattt tggtggcttg cttagccgga cctttcggaa
     8581 acagtcgata taaatagcgg ctgttacctt tttcttcgcc aaatttattc gccatcgctt
     8641 ttaccagcat acgaatcgcc ggagaagtat tgaattccag atagaaatca cgcacaaaac
     8701 gcaccacttc ccagtgttct ggcgacagcg aaatcccttc gttctctgca atcaccacag
     8761 ccagcggctc actccactgg ctgctttctt tgagatagcc ttcggtatcc gtttctatct
     8821 ctttaccttc gaagatcagc ataattatta ctaccttaat cagactggcg gcagtgtaac
     8881 aaaaaacaaa gccccgcata aagcgaggct atgaaagtgt tagcgggtga gattaatcgc
     8941 ggctagcgaa gcccagaatg ctcagcaggc tgacgaagat gttgtacagc gaaacataca
     9001 ggctaaccgt ggcacgaata tagttcgtct caccgccatg aatgatgttg ctggtttcaa
     9061 acaagatagc gccagaggag atcagaatga agaccgcgct gatcgccaga tgcagagcag
     9121 gcagttgcag gaagatatta gcaaccatac caatcagcac caccacaata cccgccatca
     9181 gcataccgcc gaggaacgac atatctttgc gggtggtcag cacatatgca gagcagcaga
     9241 agaacactaa cgccgttccg cccagtgcca tagcgattac gtcacccatt ccggcagaca
     9301 gataggtgtt cagaataggt ccgaggatat aacccagaaa accggtaaag gcgaatgcgg
     9361 agataatccc ggtcggctta ttcgccgttt tataggtcag gaacatcaaa ccatacatac
     9421 ccaccagcgt cagaatcaga cccggagatg gcagcatcag caccgtactg gcagttgcgg
     9481 taatcgccga aaaggccagc gtcaggctca gcagaaaata ggtattacgc agcaccttat
     9541 gggtgctaag cagtgatgta cggtcatgtg aagaactaac aatacgatcc attgagtcac
     9601 tctctatgac agatgtaatt aattaagcag cataatgata atgcgtaagg gcacccagaa
     9661 gttttaccca tctttacgca tttgatctgg aacaggttta acagcggatt atcaggtcat
     9721 taagcaaata taacgccctg agaatttcga caggcaaaag aaaaaggggt tagcatttag
     9781 ctaacccctt atcttatttg gcggaagcgc agagattcga actctggaac cctttcgggt
     9841 cgccggtttt caagaccggt gccttcaacc gctcggccac acttccggaa tgacgcgcac
     9901 tataaacatc ccgatgcggc gtgtaaaccc ctaatttgtt tgtttgcctg aaaaacagcc
     9961 aaaagtgcat tgatagcgtg aaataacagc agattgatca tttcatcacc atgaattcct
    10021 tctcttttac tcgtttagca accggctaaa catccccacc gcccggccaa aagaaaaata
    10081 ggtccatttt tatcgctaaa agataaatcc acacagtttg tattgttttg tgcaaaagtt
    10141 tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa ggatgagctt
    10201 tcgttttcag catctcacgt gaagcgatgg tttaccttgc tacagggacg tcgcttgccg
    10261 accatgtgcg cccggtgtcc tgccggtgtc gcaaggagaa gagacgtgcg atatgaataa
    10321 cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca gctttctcaa
    10381 atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac caaagattgc
    10441 ctgggcgctg gagaacaaac cccgcattcc ggtggtatgg atccacggtc tggaatgcac
    10501 ctgctgtacc gaatctttta tccgctccgc tcacccactg gcgaaggacg tcatcctttc
    10561 cctgatttcc ctcgattatg acgatacttt gatggctgcc gccggaaccc aggcggaaga
    10621 agtcttcgaa gacatcatca cgcaatacaa tggcaaatat atcctcgcag tagaaggtaa
    10681 tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt ttattgagaa
    10741 actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct gcgcgtcctg
    10801 gggctgcgtg caggccgcgc ggcccaatcc gacgcaggca acgcctatcg acaaagtcat
    10861 caccgacaaa cccattatca aagtacctgg ctgcccgccg atcccggatg tgatgagcgc
    10921 catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa tgggccgtcc
    10981 gctgatgttc tatggtcagc gaatccacga taaatgctat cgccgcgccc acttcgacgc
    11041 cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttact gcctgtacaa
    11101 aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct ggaatgatgg
    11161 cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa atggtttctg
    11221 ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta ctcattccac
    11281 cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcggctgttg gtgtgcacgc
    11341 agtcgccagc gccgttgacc agcgcagacg tcataaccag caacctacag aaaccgaaca
    11401 tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca gggatacacc
    11461 atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga aggccacatg
    11521 cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc ctgcggcacc
    11581 atgtttcgcg ggctggagat cattctgcaa gggcgcgacc cgcgcgatgc gtgggcgttc
    11641 gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt ttacgccatc
    11701 gaagatgcta tcggtattaa agtgccggac aacgccaata tcatccgcaa cattatgctg
    11761 gcaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg gatggactgg
    11821 atcgatgtgt tagatgcgct gaaagccgac ccgcggaaaa cctccgaact ggcgcaaagt
    11881 ctctcctctt ggccgaaatc atcccctggc tatttcttcg acgtacaaaa ccgcctgaaa
    11941 aaatttgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg gcacccgcag
    12001 tacaaactgc cgccagaagc taacctgatg ggctttgccc actatctcga agctctcgat
    12061 ttccagcgtg aaattgtcaa aatccacgcg gtctttggcg gtaaaaaccc gcatccaaac
    12121 tggattgtcg gcgggatgcc ttgcgccatc aacattgacg aaagcggcgc ggtcggggca
    12181 gtcaatatgg aacgcctgaa cctggtgcag tcaattatca cccgcacggc ggacttcatt
    12241 aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc gtggagcgaa
    12301 atcggcactg gtctttctga taaatgcgtt ctcagctacg gcgcattccc ggatattgcc
    12361 aacgactttg gcgagaaaag tctgctgatg cctggcggcg cggtgattaa cggcgacttc
    12421 aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt tgtcgaccac
    12481 gcctggtatc gatatcccaa cgatcaggtc gggcgtcatc cgttcgatgg catcaccgac
    12541 ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca gctgaatgaa
    12601 caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat ggaagtgggg
    12661 ccgctggcgc gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt tgagtcggtc
    12721 gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac gttaggccgc
    12781 attttgtgcc gcgcgcacga agcgcagtgg gccgcaggta agttgcagta tttcttcgac
    12841 aagctgatga ccaacctgaa aaacggcaat ctcgccactg cttccacgga aaaatgggaa
    12901 cctgcaacct ggccgacaga gtgccgtggt gtcggtttta ccgaagcgcc gcgcggggcg
    12961 ttaggccact gggccgccat tcgcgatggc aagattgatc tctaccagtg cgtggtgccg
    13021 accacctgga acgccagccc gcgcgatccc aaagggcaga ttggcgctta tgaagcggcg
    13081 ctgatgaaca ccaaaatggc gatccccgag caaccgctgg agatcctgcg tactctgcac
    13141 agctttgacc cgtgcctcgc ctgttcaaca cacgtgctgg gcgacgacgg tagcgagctg
    13201 atctccgtgc aggtgcgtta acagcgaagg agaatcatca tgcaacagaa aagcgacaac
    13261 gttgtcagcc actatgtctt tgaagcgcca gtccgcatct ggcactggtt gacggtgtta
    13321 tgcatggcgg tgttgatggt caccggatac tttatcggca agccgctacc ttccgtcagc
    13381 ggcgaggcga cgtatctgtt ctatatgggc tacatcaggt taattcactt cagcgccggg
    13441 atggttttta ccgtggtttt gctgatgcgg atctactggg cttttgttgg caatcgatac
    13501 tcccgcgagc tgtttatcgt gccggtatgg cgtaaaagct ggtggcaggg cgtgtggtat
    13561 gaaatccgct ggtatctgtt tctggcaaaa cgtccgagtg ccgatatagg ccataatccc
    13621 atcgcccagg cggcgatgtt cggctatttc ctgatgtcgg tctttatgat catcactggt
    13681 tttgcgctgt acagcgaaca cagccagtac gctatttttg cgccgttccg ttatgtggtg
    13741 gaatttttct actggacggg tggcaactca atggacattc acagctggca tcggctgggg
    13801 atgtggctga ttggcgcgtt tgtgatcggt catgtctaca tggcgctgcg tgaagacatc
    13861 atgtccgacg acacggtgat ctccaccatg gtcaacggct accgtagcca caaatttggc
    13921 aaaataagta acaaggagcg ttcatgagcg agcaacgcgt ggtggtcatg gggctgggca
    13981 acctgctgtg ggccgatgaa ggcttcggcg tgcgggtggc ggaacggctg tatgcccatt
    14041 accactggcc cgagtatgtg gagattgtcg atggcggtac tcagggactg aacttgctgg
    14101 ggtatgtcga aagcgccagc catctgttga ttctcgatgc cattgactac gggctggaac
    14161 ctggaacgct gcgaacctat gccggagaac gcattccggc ttatctcagc gcgaagaaaa
    14221 tgagcctgca tcagaacagt ttctccgaag tgctggcgct ggcagatatc cgcggacatc
    14281 tgccagcaca tattgccctc gtcggtctgc aacccgctat gctcgacgac tacggcggca
    14341 gcctgagcga actggcacgg gatcaactgc ccgctgcgga acaatcg
//
LOCUS       DAEVZA010000126        14131 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEVZA010000126 DAEVZA010000000
VERSION     DAEVZA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.126"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            138..344
                     /locus_tag="LAF92_003906"
     CDS             138..344
                     /locus_tag="LAF92_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBI9792835.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            438..1040
                     /locus_tag="LAF92_003907"
     CDS             438..1040
                     /locus_tag="LAF92_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792836.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(1999..3534)
                     /locus_tag="LAF92_003908"
     CDS             complement(1999..3534)
                     /locus_tag="LAF92_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBI9792837.1"
                     /translation="MNDTSSDDILLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNLLQQESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPTEPCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACRRC
                     DRIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHIPLYRQSEIYARQGVVLSRSVLSG
                     WVDACCRLLAPLDEALQHYVLTDGKLHADDMPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGHIKEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIESELRGKRAEERQAVRHQKVLPLLASLEG
                     WLREKQKTLSRHSELAKAFGYALNQWPALTRYAEDGWVEVDNNIAENALRLVSLGRKN
                     WLFFGSDHGGERGASLYSLIGTCKLNGVDPERYLHHVLDVIADWPVNRVGALLPWRVT
                     LPA"
     gene            complement(3584..3931)
                     /gene="tnpB"
                     /locus_tag="LAF92_003909"
     CDS             complement(3584..3931)
                     /gene="tnpB"
                     /locus_tag="LAF92_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI9792838.1"
                     /translation="MINLPAGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLMEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3928..4311)
                     /locus_tag="LAF92_003910"
     CDS             complement(3928..4311)
                     /locus_tag="LAF92_003910"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBI9792839.1"
                     /translation="MRAKERLPRKHYSPEFKMELVRLALEEEGSIAALARKHDVNDNL
                     LFKWIRLWQREGRVCRPRKNSSSLPALIPVQLQAGSSLPTFELPSCSPPATCHIKCRG
                     GEITLTHPSAELMSTVLRELMRGPV"
     gene            4390..4549
                     /locus_tag="LAF92_003911"
                     /pseudo
     CDS             4390..4549
                     /locus_tag="LAF92_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            4593..4748
                     /locus_tag="LAF92_003912"
                     /pseudo
     CDS             4593..4748
                     /locus_tag="LAF92_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            5879..7030
                     /locus_tag="LAF92_003913"
     CDS             5879..7030
                     /locus_tag="LAF92_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792840.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            7114..7986
                     /locus_tag="LAF92_003914"
     CDS             7114..7986
                     /locus_tag="LAF92_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBI9792841.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLNGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILLELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVMQADKTEPC
                     HEWDMAGIQPSPAEAQNIREKTEAVFRLFRPVHRVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            8204..10600
                     /locus_tag="LAF92_003915"
     CDS             8204..10600
                     /locus_tag="LAF92_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HBI9792842.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     EALELMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            10597..11502
                     /locus_tag="LAF92_003916"
     CDS             10597..11502
                     /locus_tag="LAF92_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBI9792843.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            11499..12569
                     /locus_tag="LAF92_003917"
     CDS             11499..12569
                     /locus_tag="LAF92_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBI9792844.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            12705..13388
                     /locus_tag="LAF92_003918"
     CDS             12705..13388
                     /locus_tag="LAF92_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792845.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            13404..13814
                     /locus_tag="LAF92_003919"
     CDS             13404..13814
                     /locus_tag="LAF92_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792846.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            14035..>14131
                     /locus_tag="LAF92_003920"
     CDS             14035..>14131
                     /locus_tag="LAF92_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBI9792847.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSI"
BASE COUNT         3608 a         3594 c         3745 g         3184 t
ORIGIN      
        1 aatccccccg tttatctccg gacagccaca ttcccgatta ttttttacgt ttctccccgg
       61 ttgttatgcc ggtgaaggtg gcgcgctgtt ttcatgacca caccggctgg tttaacaaca
      121 tccggaggaa cattctcatg gctaccccag tttcgctgat ggatgaccag atggtcgaca
      181 tggcgtttat cactcaactg accggcctta ccgataagtg gtttgacaaa ctcatcaaag
      241 atgggggctt tcctgcgccc atcaaaatgg ggcgcagctc ccgctggctg aaaagtgaag
      301 tggaagcctg gctgcaggcg cgtattgcac agtcccgtcc gtaattttta accactaacc
      361 cttcgcccac agcagacgca atctccggcc tgccggcggc gctctgctgc ctgttatccc
      421 ccgtgaggaa tatgaaaatg aaacaacagt atcagacccg ttacgagctg ctccatgaaa
      481 actaccagaa atggctgacc ggcttcaccc ggcacgccgt atcatggggc gtgtgtcatc
      541 cgaatatcta ctatttccat aatctgacgc ccgggtgggt gtcattcaat ggcgaaaaac
      601 cggagattgc catcgttccc cagagtctgc accggctgat ttatggtccg gacaaacgga
      661 gctccccgtc cctggatgat gatctggtgg tgaatttatg caccagcgaa cacctgctgg
      721 ttcatcatcc gatgctggaa gggatcctgc tgtctgaatg tgagcgcctg aagcaacatt
      781 ctctggcaaa caaactgatc agcctcttcc gtcagtttgg cggcacggag ttacgtctca
      841 aactggtctg gctctgctgg cttgacttaa tgaccggaaa cagccttgac gactggacga
      901 aaaacctgaa acacaaatca gaaaaagatc tggagcaatg gatcattgca cgtcagggtc
      961 agagtgaacc actgacaaat ctgatggatc agtacgtgct tatggcatac cgtacatcgg
     1021 ttgatgccgc tcacagttga tgtctgagaa ccacagggct ggcagcagaa aaccagccct
     1081 gtggtaaaat acagtaatag cggcgtacat caggcacgcc gctcactcgt ccacagatca
     1141 tatacgtcat tatactgcgc gataacggag ggcttcagca cccggtcatt tctgagcggg
     1201 tatgaatacc attgatgatg cgaagcagcc acgggcagaa acgttcttca caaccaggtg
     1261 cctccatctt cactgcagtc tgaatgtgcc ggcttgttat ccaccagaaa tggtatgctc
     1321 cggtacatgc ataccccgga gactataaac gcactgaatc atagcctccc acctcagcag
     1381 gaggcagtca gctttcaggc ggaaaaacgt tgcagttaaa gatcgtgaga atcgctgttt
     1441 cgttgcatag caacccgaac agttgtagtg aaacatttta cctgcatcag cacatggtca
     1501 ggcgccgtct gtatggcgtg gaatactgat gtttcggctg atggcaagcc tcattctctg
     1561 tggaaacagg ggcgcgcatt agcttctcaa ccagagagtg tcacaaagac aaatattgct
     1621 gaaaatcgca ttcatcgttc aggcgtgaat gcccggagac aaaagccgct gtccacacag
     1681 tccccctctg acctgaacaa aaagcataca cacaacaggc tcacctgacc ccgaaaaacc
     1741 ataacttcag caataccagt gacaccagca cgttacctca gtgccacatc ttcagaccgc
     1801 gatcaatctg aaatgtgact atcggggcaa tatccctgct ttgtggtaca cgataaccag
     1861 aaaacatccc ctgaaccgcc agccagaaag actggccaca aaaggggtaa gctgaaatcg
     1921 tttatggatt acatcgacat atgcctggca gagaatggta agcgtgcagt gagaaccgta
     1981 ttgacgaagt gaagctggtc aggcaggcag agtaacccgc cacgggagca gagcacccac
     2041 ccggttcaca ggccagtcgg cgatgacatc aagtacatga tgcagatagc gttccggatc
     2101 cacgccgttt aatttgcacg tcccgataag actgtacagt gacgcaccgc gctcaccacc
     2161 atgatccgag ccgaagaaca gccagttttt tctccccaga ctgaccagtc gtagggcgtt
     2221 ttcggcaatg ttattatcca cctccaccca gccatcttct gcatagcggg tcagcgccgg
     2281 ccactggttc agcgcatacc cgaacgcctt cgccagttct gagtgccttg agagggtttt
     2341 ctgtttctcc cgcagccacc cctccagtga cgccagcagc ggcagcactt tttggtgccg
     2401 gactgcctgc cgttcctctg ctcttttacc tctgagctcc gactcgatgg catacagctc
     2461 gccgatccgt ttcagcgcct cctccgtcag ggctgacggg gtacggacgt gaacatcgtg
     2521 gatttttcgg cgggcatggg cccagcacgc ggcttccttt atatggccgt cgcggtagag
     2581 ctcgttgaac ccggcgtagg catcagcctg cagtacgcca ctgaagcctg caagatgggt
     2641 ctgagggtgg ataccttttc tgtccgggct gtaagcgaac cacactgcgg gggccagcgc
     2701 tgatccggcg ttgcggtcgt ctcgaacgta cgtccataag cgcccggtct tcgtcttctt
     2761 attgcccggc aacagcaccg ggacaggcat atcgtcagca tggagtttgc cgtcggtcag
     2821 gacatagtgc tgaagggctt catccagcgg tgccagcaga cgacaacacg catccaccca
     2881 gcccgacagt acagaacgac tcaacactac accctggcgg gcatagatct ccgactggcg
     2941 atacagcggt atgtgctctg catactttga ggtcagcact cgggccagca gccccggtcc
     3001 ggcgatcccc cgttcgatgg ggcgcgaagg agccggggcc tgaacgatgc gatcgcaccg
     3061 acggcaggca tgcttttcac ggactgtccg gataacccgg aaggcgctgc gcatcagctc
     3121 cagctgttcg gcggcatcct cacccaggta actcagcgaa ccaccgcact ccgggcaaca
     3181 cggctcagtc ggcagcagcc gcttctcatc ccgtgggagt gactcaggga acggtttgcg
     3241 ggtgcgggtc tgacgcagcg ggcgctgcac tgccgggtca tccacccggc cggtcagcgt
     3301 atcgctttcc tgctgcagca ggttcaggtc agcttccatt tgcgcgatac ggcgggatac
     3361 cttttcggaa cgactgccga agttcatccg gcgcagctta tccagttgcg cctgcagatg
     3421 gtctatttcg cgttcccggt tgctcagctt ttcctgcagg gcgtggatca acgcttcctg
     3481 ttcggccagg cgctgtttca gcagaaggat gtcgtcagaa gaggtgtcgt tcataagcct
     3541 gtattttacc aggcttattc tgcgacaacc aggataaaga gcgctacaac atggtcaggg
     3601 atgtcagtaa ccgctttggc tgtcgccagt cgataccttc catcagcatc gccagttgcg
     3661 ccggcgtcag gaacactttg ccatcgcggg ctgagggcca ggcgaagcga ccacgttcta
     3721 gtcgctttgt cagcaggcac agaccatcgc cggtggacca gagcagtttt acctgactgc
     3781 cgctgcgtcc ccggaagatg aagacgtggc cggacatcgg gtcatctttc agcgcggtct
     3841 gcacctttgc ggcgaggcca ttgaagccgt tgcgcatatc ggtgataccg gcaaccagcc
     3901 agatttttgt gcctgcggga agatttatca tacaggcccc cgcatcagtt cgcgcaggac
     3961 agtgctcatg agttcagcag agggatgagt cagtgttatt tctcctcccc gacattttat
     4021 gtggcaggta gcgggaggag agcaggatgg tagttcgaag gttgggagag aagagcccgc
     4081 ctgaagctgc acgggtatca gggcaggaag cgacgatgag ttttttcggg gccgacagac
     4141 ccgcccttca cgctgccaga gccttatcca tttaaagagc aggttatcat tgacgtcatg
     4201 tttccgggcc agtgcggcaa tactgccttc ttcttcaaga gccagcctga ccagttccat
     4261 tttgaattca ggggaatagt gtttccgggg aagtctttct ttagcgcgca tgaggtgtcc
     4321 acctgcaaaa taagtgggtg cttaagttac aggatcctgg attcgggacc agacggtact
     4381 cagacgacgc ttacagagaa tgtccggaat gacgaggtta tctttgcgga gattaaggca
     4441 atggtatacg ggccgacgtt ccatgccgcg ttactacatt cagcccggac gtaaaatgcg
     4501 cccgtcaaaa agaacggttc gcttcgaaac tcagccagga taccacctct ccgccacacc
     4561 cgggaagaag ttgatatggt ggttgccgga caacagcgct cctggattga ctgctgcatc
     4621 actcaaccac gctgaatatc aaaggagagc gttaccggtt aaaagagaaa cgtaaagctg
     4681 tggtgatgac ccaaaacgca acgcctgtca gtgaggatga aatggtgaaa agcggacagc
     4741 atcagtaacg gaagtatctt agcgggtacg aaaatggcag gatcgtgaca ttgacagaat
     4801 atctcctgat catcctgaat gactgcagtt gctgtagaaa agtaagctgt ctccacccac
     4861 ggcaccactt aacaggcatt ccgtaacttc ccccagactt actatgacgc attatcggtg
     4921 cactgaaaac gggagagtgc gcttaaaacc gcagaccgca tgcgggtgtt actgcatgac
     4981 agtctggccg tattgtgaga tgtcggcagg aaccgatgtc cgcgcagggg ggaacttcct
     5041 gcagtacctg acgcttcaaa tggtattaac cgtagggtgt tgttggttcc ggatcgcgga
     5101 aatgggagca caggtctgct acggggtgta cctctgacag tatatccggc tcgctcatct
     5161 gacagcctct ctcccggaaa agaccaaaaa tgagatcgtc tcctcaacag cagagaagag
     5221 gtcttgacag acaggctaca ttgcatacga tttatgaaga aaaagacagc aaatatggag
     5281 gttatagcgg agaataccct acgaaattcc tatgaaaaac gattgaaaaa aatatcaaat
     5341 tcgattcgtt tttatatgct ttttgggagc gtggcgatga cgaactgttg catgtgcaga
     5401 ggagaatgca cccggaagca ccagaatcat ccagaaaggc agaacgatta cgcatcaaac
     5461 caggagacag caatatcaag gtaatatact gatttaaaat gaatttacag caatacgtaa
     5521 aaacattttt tacttaaagg agacagatgc gctgaagaga gtgcaggtat ttttatataa
     5581 aaatgagaag tttacgggat gtatatccgg cacactatga ccggagaata tcagtcaagc
     5641 ttaaaaagac aataaataca gtgagttaaa tacacagaga aaacgtagta tcagagggta
     5701 catcttacaa cagagactga atttccggtt tttcataacc ggcgcattcc ggaaagtgca
     5761 ggcggcccgg aggttttctg acgccactac ctggtagatt gttctgtaca tcatgtacgg
     5821 aggtttttgt ttgctttttt aaaatctgca aatgtgagaa aagcaggtga ggtgtattgt
     5881 gggaaaggaa ctggatgata tctgcaccag ctgtccgtat atcgatgctg taaaacggca
     5941 caaacaacag ctcggtgcca tagaggaata tacacagtgg ttaaaaaaag aaccacgggc
     6001 atcttatttt tttctgttcc agctgtatac ccgcattcac aatacatttt tctcccaaaa
     6061 gcaacaactc ccgttcactc ccggtggcac tcactgcccg gaacctgatg tcacccttcg
     6121 ggacttaacg ctttcccctg gctaccacag tgattatgcc cctcaaccca ttccggagat
     6181 ggacagttct gctgttgttc ccccaactaa tgaaaacacc tcgcctccgg aagatactcc
     6241 cgacaacact ccggccggag gcaataccgg acaagcggaa aagaccagaa acgcaggact
     6301 cacacccatt cctgaaaaac gttcaggaat gcccccggaa catctgcggt ttgccacggg
     6361 gtttccatcg caaccgaaga ttgccggtcc cagagaaaag ccgatgcgca ccgtgcatcc
     6421 ggataaaatc taccgtgaaa ttatatggtt ctgctccagt tacctgctga aatccggacc
     6481 agaggctacg aggacgatta tcaactcagt attctctgaa tgggccagca taaacaatga
     6541 ttacccctcc cccttttcgt gggtggacag cagggacagt gaacagtgtg actggttatg
     6601 gaacgccatg caggtccggt gtgtgggaac cccgctgaat ccccttaccc cggagcagaa
     6661 atactggttt gcctgcgcca cgtttgataa ctgggagggc tggaatgagc aacaggtaca
     6721 gtttttactg gaaagtaatc ccagacggaa cagagcgaag tttacgcagg tctccttcca
     6781 ggcccccagg attcagcata aagcgattct tcttgatgag ctgaaaagtg cccgggagca
     6841 acaaaaaagg cgtgatgaac gtgctgatgg ttccgtccct ctgaaactgt ccggaaaaat
     6901 ccacaaacag cttgaaagta ttgcccgaag tcgtggtgtc ctcccaaaaa aactgctgaa
     6961 tgaaatgatt gagcaggcgt accatgattt ggtggcaacc aggcagaaca gccaaatcga
     7021 ttccagataa tttcagacag atattatttc cgtgaatccc ccgccacctt tccggtgcgc
     7081 ggggttttgt cttttttcac cgggaataca tgtatgaatc cgtctgatgc cattgaggca
     7141 attgaaaaac cgctctcctc cctgccttac tcactttccc gtcacatcct ggaacatctg
     7201 cgcaaactca ccagtcacga acccgtgcct ggcattatgg gtaaaagcgg ggccggtaaa
     7261 tcctcactct gtaatgcgct gtttcagggg gaggtcaccc cggtcagtga tgttcacgcc
     7321 ggcacccggg aagtgcagcg cttccgtctg aatggccatg gtcacagcat ggttatcact
     7381 gacctgcccg gggtgggcga gagccgggac agggatgcag agtatgaagc cctgtaccgt
     7441 gacattctgc tcgaactgga cctggtactg tggctgatta aagccgatga ccgtgccctg
     7501 tctgtggatg agtatttctg gcgacacatc ctgcaccggg gacatcagcg ggtactgttt
     7561 gtggtgatgc aggccgataa aacggagccc tgccatgaat gggatatggc cggtattcag
     7621 ccttctcccg cagaggcaca gaacattcgc gaaaaaacgg aggcggtatt ccgtctgttc
     7681 cggcccgtac atcgggttgt tgccgtatcg gcccgcaccg gctgggaact ggatacgctg
     7741 gtcagtgcgc tcatgacagc acttcccgac catgccgcca gtcccctgat gacccgcctg
     7801 caggacgagc tgcgcacgga gtctgtccgg tctcaggccc gtgaacagtt taccggtgcg
     7861 gtggaccgga tatttgacac ggcggagagc gtctgcattg cctctgttgc acgcacggtt
     7921 ctgcgcgccg tccgtgactc ggtggtctct gttgcccgcg cggtatggaa ctggattttc
     7981 ttctgaaccc ggtggtgatg taccaggctg ttgttacaga tttttaactc catgaaaaca
     8041 ctatgcaaac gcagccatac ccgggttgtc cccttgcgtt gtgtttctca agttaataga
     8101 atacatcgat cgttactatc atatcgatcg ttataaacga tcgtttgtag taaatgacct
     8161 cctccgtggt tgtcatgaaa atgcgtctta ggaaggttaa ataatgcacg aaaagaacat
     8221 cgccctgctt tgtgatgaag ccgaccgact tttgcaactg aacattaatc tgctccggca
     8281 aatggttgag gagccagatg tgttatctga cagtaagaac gaaaacagac tgctttttga
     8341 taaacagaaa gcactgaaaa gaattgagga gctggagggc gaacaaatca aaaccgcccg
     8401 cagggagatg gtgctggctg ttgtcggcac gatgaaagca ggcaaatcaa ccaccatcaa
     8461 cgccattgtg gggcaggaaa ttctgcctaa ccgtaaccgc cccatgacct ctgtaccgac
     8521 gctcatccgc cacgttcccg gaaaaactga gccggttctc catctggaac atattcagcc
     8581 tgtccgcaat ttattaatca cactgcagga aaaactcgcc accccggcag gacagcaggt
     8641 cgcacagacc ctgcagcaaa ccggggatac ccgcgaactg ctggatattc tgacggatga
     8701 tggctggctc aaaaatgaat accacgggga ggaggaaatc tttaccggac tggcatcgtt
     8761 aaacgatctg gttcgtcttg ctgcggcaat ggggactgaa tttccttttg atgaatacgc
     8821 agaagtgcag aaactgccgg tgatcgacgt ggaattcagc catctggtgg ggatggatgc
     8881 atgccaggga acactcacac tgctggatac ccccggccct aatgaggccg gacaaccgca
     8941 gatggaagtg atgatgcggg atcaactgca gaaagcctct gcggttctgg ctgtgatgga
     9001 ttacacccag atgaactcaa aagcggatga agacgtccgt aaagagctta atgccattgc
     9061 tgacgtatca gccggccgcc tgtttgtact ggtcaataaa tttgatgaga aagaccgcaa
     9121 tggcgatggg gcagatgccg tacgccagaa agttccggca atgctgaaca gcgatgtgct
     9181 gcccgcctcc cgcgtttatc ccggatcctc acgccaggca tacctggcta accgtgcgct
     9241 tcatgagtta cggaaaaacg gaacccttcc tgttgatgaa gcctgggtcg atgattttat
     9301 cagaggtgcc ttcggttgca tgaaaaaaga atacgtctgt aaagacagtg aactggcaac
     9361 tgaaggggcg acagacctgt gggaaggctc acttatcgat caactgataa cggaagtcat
     9421 acagagctca cattccagag ctgcggcact ggcggttgac tctgccgccg caaaactgat
     9481 gcagaatgca gaaaatatca gtgaatacct gttgttacgc catcaggggc tacagcaaag
     9541 cattcagtca ctgcagtcgc atatcaccag cctgcttgcg gatatccggg aaatcgcgga
     9601 ctgtcaggag cagatgacca ctgatgtcag aatggccatg gaggagatcg atactaaaac
     9661 ccgggaatta ctgacggggg tctgcacctc actggaagag gagctgaatg actatttcag
     9721 aagcggtaaa cgcaaagaac agcaaatgct ggaggaagaa gacgcagaac aacgaaggtc
     9781 tcagtccgga ttatggggga aaatttctca atggtccggt atcaacaacc agggccggga
     9841 ggattacagg aaacgagatt ttgccccgga cagccccgaa ataaaattca gtgatcgcag
     9901 ggaagccctt gaactgatga cgcaaatcga atcgaccgtg accagcctgc accgtgaggc
     9961 tgaagcacag ttccggcctg agctggagaa aatcgtcagc gggattgaaa caggttttcg
    10021 tggcacggcc ctgtacgcca cagaaaacat tgccggtcgc atcaatgccc gcctggagga
    10081 tgagggcttt accgtaaaaa tcagttttcc ggcagtcagc cagttacaga cccggctcgc
    10141 ggtaaaaata aatctgagtg cgcttatgga ggaaagaacg gagaccgtca cccgtcgccg
    10201 tcggcagagt ggcgtatggg gaaccgtttg tcgatggttt ggcaccagtg acctgggctg
    10261 ggaaaactat gacgaggatg tgagtcgcag cgtgatcaat atcaacaagg tcagagagga
    10321 agttatgtca ctgacccggg catatttcgg ggagctgcag gcatccattg agcaggatat
    10381 taaccagccc gtccgccagg aaatcgatgc ctttttctgc gcattcaggg agaaagttga
    10441 acaactgcgt aacacgctga ttcagagctc tgaagatcat aaacgcgatc agcaggcgca
    10501 ggaacggctt accgggcgac ttcaggcatt aaatgaaagg gttcctgagc tcattactga
    10561 cagtaaggcg ctcagggaag agctggagac aatgctgtga cttcaccatt tattcaacaa
    10621 attgctgata accgggtatg tcaggtactc acctgtcttc ctgaaaaatt tgtggttgat
    10681 ttcgcaaacg gaatcgatgt tgcacaggag catatccgca cggccggaga gcgcacgttc
    10741 ttccggcgtt taaaagaggg gctgaccggc gaaggcgctg cacggcagaa tgccatcaat
    10801 gcgtcgcttg cacagggcgt tgaggcgtcc ctgcgctggc tgacggagat gacaacgtca
    10861 cttgccacca caaactacgc gattacccgg gtaaacgaca gggtcagttc actggtcagt
    10921 gatacagcca ggctggcgca ttattccgca gatacgcggg agcagttact caccctggcc
    10981 gatcaggttc accataaact gaatcatctt gaagaaaaac tccaccgtgt tgaccaggtt
    11041 cagcgggcgc agttacatct tgagcagata ttctcatggt ggagcgccgg gcgatatgcg
    11101 tcattttccc ctgccggacg ttgttatgtg gcacttgaag agcttcgctg gggcgcgttt
    11161 ggtgatgtga tacgtcagag cgaaacaggc caggttaacc agctactgga tattctcaga
    11221 cataaagcat taacgcagat ggcacaggag agtggcggta gtgcaaccgt gcgtctgaac
    11281 actctggact ggcttggtgg tcagggccgt gagcaggcag ataacgagtg gcatgacgcg
    11341 attaactggc tgggagactg gtgcagtgaa gagcagcatc cggtgatctg gtcaaccacg
    11401 caggctgcag aacacttacc ggttcgcatg cctcgcctct gctctgcaga acgcctctct
    11461 gaaagtatgg tcgatgaaat atttcagaag ggggctgcat gagtacagaa atgaaaacgg
    11521 ggctggtgct gtccggtggc ggggcggtgg gcgcttatca ggcgggagtg gttaaggcac
    11581 tggcagagtg tggtacacag atcagcatgg tttcaggggc cagcattggc gcattcaatg
    11641 gtgccattat cgcggcctct cccgatctgt cagaagctgc cgtacgcctg gaggcgctct
    11701 gggatcatct ggggaataat caggtgctgt cggtaaacag attggtttac ttttcattgc
    11761 tgaaaaaatt gttccaggca atgaacctct gccagatccc cggacgtgca ggagcactgc
    11821 ttacgacgct tcttcgccat atatcgacaa tcaacgggtt tgacaatctg atggctcagc
    11881 cgttgttgtc agatgagccc ctgacagcgc tgatggatca ttatcttgat actgatgctc
    11941 tggcagacgg gctaccgctg tatgtgtcgc tgtaccccac agaagggggc atgcaggata
    12001 ttattgactg cattcgtgct gaactgggtg tcggaaccac gaaaaacgcc gtttttcagc
    12061 atatccagag cctgccccgc ggacagcaga aagaggctct gcttgcgtca gccgcgctgc
    12121 ccctgctgtt ccgtccccgt gaggttcagg ggacaatgtt cggtgatggt ggtatgggag
    12181 gatggcgaaa tatgcaggga aatacccctg tgacgcctct ggtcgatgcc ggatgcaata
    12241 tggtgattgt gacgcatctg agtgacggtt ctttatggga tcgccaggct tttccggaca
    12301 ccacaatcct tgagatccgt ccccggaaaa ggctgaaata tgcaggtgat ggtggcaaca
    12361 gcggcggtct gctcagtttt acatcggcac ataccgacgc ctggcgtcag cagggctatg
    12421 aagacacgat gctggcgatg gagcatatcc ggaaaccgct ggcagcacgt caggcactga
    12481 cccggtcaga ggcggtattg cagaaaagcc tggatataac ggaagaggca gatttggcac
    12541 tgagaaacgc gatggcccgg attaaataaa gacgccccaa agaaacgcca ccgcctgtgg
    12601 tggtgttttt cctgtctggt gttattgaaa atattttcat atatatatta cctgagagaa
    12661 agttttaccc ctcacctgtc aattcactca ctggaaattt aattatgaaa ttacctgtaa
    12721 agttattaat gagccttata tctctggtca gcgttattgc acgtgccggg gaatataaaa
    12781 attacagccg ggatgaaata aaatactggc gatatacatc atacaagggg gggaaacttc
    12841 cggaaggttt cactgatgag aaattttcca gcgccattta caacggaaga atatttacaa
    12901 tgaaacgttt acataccctt atgttatttc tggcggttct gttcacgggc tttaacttta
    12961 acgcagaagc ggctaccgta aaacaagctc ttagttgtaa cccggaagcc tgggctgaac
    13021 aacctggagc gtgtccatca acgtacgagt tgtacgaagg tgacgctacc tacaaagccg
    13081 ctatcgacaa agcattaaaa ccggtcggac tgagcggcat gttcggtaaa ggcggctata
    13141 tggatggccc tggtggaggt atcacgccag taaacattaa cggtacagtc tggttccagg
    13201 gcgacggttg caaagccaat acctgcggct gggactttat cgtaacactc tataacccaa
    13261 aaacccatga agttgttggc taccgttact ttggtttaga tgacccggcc tacctggttt
    13321 ggttcggtga aattggcgtg catgaattcg cgtatctggt gaaaaactac gtagctgcgg
    13381 ttaactaatt taaggagtct atcatgaaaa cttttatcaa aactttactc gttgctgtaa
    13441 ctattctgtt ctctgtcttc gctactgcga aacaagtaaa actgccaaat aacatcaaat
    13501 acgttaatac tacagaagcg ttttcctgta ccgaaattga cggtatgaat tgccagacaa
    13561 agaatcagtt caactataaa gataacagct atgttttcgt gcttgaacgt ggtggtgcct
    13621 ggtgctacga ctacactgtc tcggtagtta acctgaaaac cgggaaagca caaatgattg
    13681 aatacgggga caatcaactg tgctcaggta gcaacaaacc gttcttcgaa atcaaaaatg
    13741 gcgtaccgac ggtaggagtc atcgacacat ccggaaaacc tgtcgttgta gcccaggaca
    13801 aacttaaaat ctaaggagta aaccatgcgt ttctctgcac attattaata tcaaaggtaa
    13861 atacagcccc agccattgtt accggaaagc cgcttaatgc gtggctcgcc agtgaaaggc
    13921 ctctcagaca ataaacctta ttcatttcac ccgtcaggcc gcgtcttctc cgggagacgc
    13981 ggcttttttc atttattgca ccagtaaatc ttaaccaccg ataaggagca aagtatgcga
    14041 ttagcaagtc gttttggtta tgctgcaaac cagatacgcc gtgaccgtcc gctgacacat
    14101 gaagaactga tacgccatgt acccagtatt t
//
LOCUS       DAEVZA010000127        14124 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEVZA010000127 DAEVZA010000000
VERSION     DAEVZA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.127"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..716
                     /gene="codB"
                     /locus_tag="LAF92_003921"
     CDS             <1..716
                     /gene="codB"
                     /locus_tag="LAF92_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBI9792848.1"
                     /translation="KAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFGRNAKLAVL
                     VAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNIWTTNDNAL
                     YASGLGFANITSMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPPVGGVIIAD
                     YLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGALSYLILNP
                     ILNRKTTAAMTHVEVNSVE"
     gene            706..1989
                     /gene="codA"
                     /locus_tag="LAF92_003922"
     CDS             706..1989
                     /gene="codA"
                     /locus_tag="LAF92_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HBI9792849.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(2029..2928)
                     /gene="cynR"
                     /locus_tag="LAF92_003923"
     CDS             complement(2029..2928)
                     /gene="cynR"
                     /locus_tag="LAF92_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HBI9792850.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            3038..3697
                     /gene="cynT"
                     /locus_tag="LAF92_003924"
     CDS             3038..3697
                     /gene="cynT"
                     /locus_tag="LAF92_003924"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HBI9792851.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            3728..4198
                     /gene="cynS"
                     /locus_tag="LAF92_003925"
     CDS             3728..4198
                     /gene="cynS"
                     /locus_tag="LAF92_003925"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HBI9792852.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            4204..5385
                     /gene="cynX"
                     /locus_tag="LAF92_003926"
     CDS             4204..5385
                     /gene="cynX"
                     /locus_tag="LAF92_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HBI9792853.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPMQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGLLRSISGNYLMDWAFHTLCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(5464..6717)
                     /gene="lacY"
                     /locus_tag="LAF92_003927"
     CDS             complement(5464..6717)
                     /gene="lacY"
                     /locus_tag="LAF92_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HBI9792854.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(6769..9843)
                     /gene="lacZ"
                     /locus_tag="LAF92_003928"
     CDS             complement(6769..9843)
                     /gene="lacZ"
                     /locus_tag="LAF92_003928"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBI9792855.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TVRPSPQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVIVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLLWCQK"
     gene            complement(9966..11048)
                     /gene="lacI"
                     /locus_tag="LAF92_003929"
     CDS             complement(9966..11048)
                     /gene="lacI"
                     /locus_tag="LAF92_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HBI9792856.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(11125..12072)
                     /gene="mhpR"
                     /locus_tag="LAF92_003930"
     CDS             complement(11125..12072)
                     /gene="mhpR"
                     /locus_tag="LAF92_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HBI9792857.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            12149..13813
                     /locus_tag="LAF92_003931"
     CDS             12149..13813
                     /locus_tag="LAF92_003931"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HBI9792858.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            13815..>14124
                     /gene="mhpB"
                     /locus_tag="LAF92_003932"
     CDS             13815..>14124
                     /gene="mhpB"
                     /locus_tag="LAF92_003932"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="catalyzes the cleavage of 3-(2,3-dihydroxyphenyl)
                     propionate into 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate;
                     part of the 3-phenylpropionic acid degradation pathway;
                     member of the protocatechuate 4,5-dioxygenase family;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBI9792859.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     I"
BASE COUNT         3169 a         3822 c         3887 g         3246 t
ORIGIN      
        1 taaaagcggt cgttcccgca caaccgttag atttcaatgt cgcgctggcg ctggttgtgg
       61 ggtcatttat cagtgcgggt acgctcaccg ctgactttgt ccggtttggt cgcaatgcca
      121 aactggcggt gctggtggcg atggtggcct ttttcctcgg caactcgttg atgtttattt
      181 tcggtgcagc gggcgctgcg gcactgggca tggcggatat ctctgatgtg atgattgctc
      241 agggcctgct gctgcctgcg attgtggtgc tggggctgaa tatctggacc accaacgata
      301 acgcactcta tgcgtcgggt ttaggtttcg ccaacattac cagtatgtcg agcaaaaccc
      361 tttcggtaat caacggtatt atcggtacgg tctgcgcatt atggctgtat aacaattttg
      421 tcggctggtt gaccttcctt tcggcagcta ttcctccggt agggggcgtg atcatcgccg
      481 actatctgat gaaccgtcgc cgctatgagc actttgcgac cacgcgtatg atgagtgtca
      541 attgggtggc gattctggcg gtcgcgctgg ggattgccgc aggccactgg ttaccgggaa
      601 ttgttccggt caacgcggta ttaggtggcg cgctgagcta tctgatcctt aacccgattt
      661 tgaatcgtaa aacgacagca gcaatgacgc atgtggaggt taacagtgtc gaataacgct
      721 ttacaaacaa ttattaacgc ccggttacca ggcaaagagg ggctgtggca gattcatctg
      781 caggacggaa aaatcagcgc cattgatgcg caatccggcg tgatgcccat aactgaaaac
      841 agcctggatg ccgaacaagg tttagttata ccgccgtttg tggagccgca tattcacctg
      901 gacaccacgc aaaccgccgg acaaccgaac tggaatcagt ccggcacgct gtttgaaggc
      961 attgaacgct gggccgagcg caaagcgtta ttaacccatg acgatgtgaa acaacgcgcc
     1021 tggcaaacgc tgaaatggca gattgccaac ggcattcagc atgtgcgtac ccatgtcgat
     1081 gtttcggatg caacgctaac tgcgctgaaa gcaatgctgg aagtgaagca ggaagtcgcg
     1141 ccgtggattg atctgcaaat tgtcgccttc cctcaggaag ggattttgtc gtatcccaac
     1201 ggtgaagcgt tgctggaaga ggcgttacgc ttaggggcag atgtagtggg ggcgattccg
     1261 cattttgaat ttacccgtga atacggcgtg gagtcgctgc ataaaacctt cgccctggcg
     1321 caaaaatacg accgtctcat cgacgttcac tgtgatgaga tcgatgacga gcagtcgcgc
     1381 tttgtcgaaa ccgttgctgc cctggcgcac cgtgaaggca tgggcgcgcg agtcaccgcc
     1441 agccacacca cggcaatgca ctcctataac ggggcgtata cctcacgcct gttccgcttg
     1501 ctgaaaatgt ccggtattaa ctttgtcgcc aacccgctgg tcaatattca tctgcaagga
     1561 cgtttcgata cgtatccaaa acgtcgcggc atcacgcgcg ttaaagagat gctggagtcc
     1621 ggcattaacg tctgctttgg tcacgatgat gtcttcgatc cgtggtatcc gctgggaacg
     1681 gcgaatatgc tgcaagtgct gcatatgggg ctgcatgttt gccagttgat gggctacggg
     1741 cagattaacg atggcctgaa tttaatcacc catcacagtg ccagaacatt gaatttgcag
     1801 gattacggca ttgccgccgg aaacagcgca aacctgatta tcctgccggc tgaaaatgga
     1861 tttgatgcgc tgcgccgtca ggttccggta cgttattcgg tacgtggcgg caaggtgatt
     1921 gccagtacac aaccggcaca aaccaccgta tatctggagc agccagaagc catcgattac
     1981 aaacggtaat ttcctctgtg ttgaagagcc actgacctgt gtcagtggct accgtgattc
     2041 attctcgcca acatccgcac attcctccaa cgccatgtgc aaaaatgcct tcgcggcggc
     2101 tgtctgccag ctatttttcc gccgcaacaa aaccgccgtt ctctccagta gtggcggggc
     2161 aagagaaatc gctttaagcc cgtcatgttg tgtggcaatc gctgctggta acaatgtgga
     2221 aagggaagtg cggcgaatca gctccagaac cgcgctaatt gagttcgcct caatgaccac
     2281 ctgtggatgt agccccgctt tctcgcagta gtggtcaatt tgctctctgg tggcaaattc
     2341 cgcgctgagc aggaccagtt tttcatcatg caagcgactc aacgccacct gttcacaggc
     2401 agccagcgga tgatgttgcg ccacgactaa cgctaaactt tctgtcagta aaggaattgc
     2461 ctccagctcc ggcgaatgca caggcgcgaa ggcaatccca acgtccagct cgtcgcgaca
     2521 aagcagatct tcgattttct cctgcgacat ttcctgtagc tggagcgtga tgccggggta
     2581 gcgcgcatag aaatccgcca ttaaagggcc gataaagtag cttgtaaagg tgggagtgac
     2641 ggcgatacgc agcgatcctc gcgtcagatc ggcaacatca tgaatcgccc gtttacccgc
     2701 ccccagttcc tgtaacgccc ggctggcgta ctgtcgccag acttctcctg catcagtgag
     2761 acgaatcgtt cgcccgctac ggtcaaacag cggcacgcct aaactctcct ctaactggcg
     2821 aatctgctgg gaaagcgcag gctgggagac gtgcaacgca ctggcggcac gggtgaagct
     2881 gccatgttca gccacggcaa gaaaataatt gatatgtcga gagagcattc gccaacctat
     2941 aagtaaatcc aatagaactc atcataaatg agacttttac cttatgacaa tcggcgagta
     3001 gtctgcctct cattccagag acagacagag gttaacggtg aaagagatta ttgatggatt
     3061 ccttaaattc cagcgcgagg catttccgaa gcgggaagcc ttgtttaaac agctggcgac
     3121 acagcaaagc ccgcgcacac tttttatctc ctgctccgac agccgtctgg tccctgagct
     3181 ggtgacgcaa cgtgagcctg gcgatctgtt cgttattcgc aacgcgggca atatcgtccc
     3241 ttcctacggg ccggaacccg gtggcgtttc tgcttcggtg gagtatgccg tcgctgcgct
     3301 tcgggtatct gacattgtga tttgtggtca ttccaactgt ggcgcgatga ccgccattgc
     3361 cagctgtcag tgcatggacc atatgcctgc cgtctcccac tggctgcgtt atgccgattc
     3421 agcccgcgtc gttaatgagg cgcgcccgca ttccgattta ccgtcaaaag ctgcggcgat
     3481 ggtacgtgaa aacgtcattg ctcagttggc taatttgcaa actcatccat cggtgcgcct
     3541 ggcgctcgaa gaggggcgga tcgccctgca cggctgggtc tacgacattg aaagcggcag
     3601 catcgcagct tttgacggcg caacccgcca gtttgtgcca ctggccgcta atcctcgcgt
     3661 ttgtgccata ccgctacgcc aaccgaccgc agcgtaacct tatttttaaa ccatcaggag
     3721 atccaccatg attcagtcac aaattaaccg caatattcgt cttgatcttg ccgatgccat
     3781 tttgctcagc aaagctaaaa aagatctctc atttgccgag attgccgacg gtaccggtct
     3841 ggcagaagcc ttcgtaaccg cggctttgct ggggcagcaa gcgcttcctg ccgacgccgc
     3901 ccgcctggtc ggggcgaagc tggatctcga cgaagacgcc attctactgt tgcagatgat
     3961 tccactgcgt ggctgcattg atgaccgtat tccaaccgac ccaacgatgt atcgtttcta
     4021 tgaaatgttg caggtgtacg gcacaaccct gaaagcgttg gttcatgaga agtttggcga
     4081 tggcattatc agcgcgatta acttcaaact cgacgttaag aaagtggcgg acccggaagg
     4141 tggcgaacgt gcggtcatta ccttagatgg taaatatctg ccgaccaagc cgttctgaca
     4201 gccatgcgca atcatcaaaa gacgttcacg atgctgctgg ttctggtgct gattggtctt
     4261 aatatgcgac cactgctcac ctccgtcggg ccactgctac cgcaattgcg ccaggcgagc
     4321 ggaatgagct ttagcgtggc tgccctgttg accgctcttc cggtggtcac catgggcggg
     4381 ctggcgctgg ccggaagctg gcttcatcag catgtcagcg aacgtcgcag tgtcgccatc
     4441 agtctgttgc tgattgccgt cggtgcattg atgcgtgagc tttaccccca aagtgcgctg
     4501 ctgcttagca gcgcactgct tggtggggtg gggatcggca tcattcaggc ggtgatgcct
     4561 tcggtgatta aacggcggtt tcagcagcgc acgccactgg tgatggggct gtggtccgcg
     4621 gctctgatgg gcggcggtgg acttggtgcc gccataacgc cctggttagt tcaacatagc
     4681 gaaacctggt atcaaacact cgcctggtgg gcgctgcctg ccgttgttgc gctctttgcc
     4741 tggtggtggc aaagcacccg cgaggtcgcc tcttcccaca agacaacaac cactccggtt
     4801 cgcgtggttt tcactcctcg cgcgtggacg ctgggtgttt actttggtct gattaacggt
     4861 ggttacgcca gcctgattgc ctggttaccc gccttctata ttgagattgg tgccagcgcg
     4921 cagtacagcg gttccttgct ggcattgatg acgcttgggc aagccgcagg agctttgctg
     4981 atgcctgcta tggctcgcca tcaggatcgg cgcaaactgt taatgctggc gctggtgtta
     5041 caactggtgg ggttctgcgg ctttatctgg ctgccgatgc aattgccggt attgtgggcg
     5101 atggtgtgtg ggttaggtct gggcggcgcg tttccgctct gtttgctgct ggcgctcgat
     5161 cactctgcgc aaccggctat tgctggcaag ctggtggcgt ttatgcaggg gatcggtttt
     5221 atcatcgccg ggcttgcccc gtggttttct ggtttgctgc gtagtatcag cggcaattac
     5281 ctgatggact gggcatttca tacgctgtgc gtcgttgggc tgatgatcat aaccctgcgt
     5341 tttgcaccag cacgttttcc gcagctgtgg gtcaaagagg tatgatgcga cgcttgtttc
     5401 gccagatgat ggagctataa ataattgtcg gatgtgccca gtggcgcatc cgacattaat
     5461 tgcttaagcg acttcattca cctgacgacg cagcagggaa agcgggccgg ggccgctaag
     5521 cgtgaacacg gaaattaagg tgaagcccag cgccaccaga cccagcacca gataagcgcc
     5581 ctggaaaccg atgctttcat acatattgcc cgccagtaca gacataaaaa tcatcgccag
     5641 ttgcttaaag aagcagaaac agaccagata aatcgtcgct gaaaaacgca cttcaaactg
     5701 gctggtaata tatttaaagc agcccaccag caggaacggt acttcaaaca tatgcagcgt
     5761 tttcagaata accacttcca gcgctgaggt ggcgaacgat gagccaataa tacgtacaga
     5821 cataatagtg ccagccagca gcagggcgtt tttcccaccg atgcgattaa tgatcagtgg
     5881 cgcaaagaac ataatcgagg cgttaagtaa ttcgcccatt gtcgttacgt agccaaatac
     5941 ccgcgtaccc tgttcaccgg tagcaaagaa cgaagtaaag aaattagcaa actgttggtc
     6001 aaaaacatcg taggtgcagg aaacgccaat aacatacagt gacaaaaacc acagttttgg
     6061 ctgtctgaac agttccagcg ccagcttaag gctaaatgcc gaatggttgg cacctaccgc
     6121 attggcaacc gtggcggaag agggcgcatc cgttttggcg aaaaagagta aaacggcgag
     6181 gatgagtgca cagccagaac ccagccagaa aacaaactga ttattgatgg tgaacatgat
     6241 gccgacaatc gaggcacaca gcgcccagcc aacacagcca aacatccgcg cgcgaccaaa
     6301 ttcgaaatta ctgcgacggc tgactttctc aataaatgcc tctactgctg gcgcaccggc
     6361 gttaaaacag aaaccaagat aaataccacc aacaatcgac cctactaaaa tgttgtattg
     6421 taacagcggg ccgaagataa aaataaagaa cggcgcaaac atcactaaca tgccggtaat
     6481 aatccacagc aggtatttgc gcagcccgag tttgtcagaa agcagaccaa acagcggttg
     6541 gaataatagc gagaacagag aaatagcggc aaaaataata cccgtatcac ttttgctgat
     6601 atggttgatg tcatgtagcc aaatcgggaa aaacgggaag taggctccca tgataaaaaa
     6661 ataaaagaaa aagaataaac cgaacatcca aaagtttgtg ttttttaaat agtacataat
     6721 ggatttcctt acgcgaaata cgggcagaca tggcctgccc ggttattatt atttttgaca
     6781 ccagagcaac tggtaatggt agcgaccggc gctaagctgg aattccgccg acactgacgg
     6841 gctccaggag tcgtcgccac caatccccat atggaaaccg tcgatattca gccatgtgcc
     6901 ttcttccgcg tgcagcagat ggcgatggct ggtttccatc agttgttgtt ggctgtagcg
     6961 actgatattg aactggaagt cgccgcgcca ctggtgtggg ccataattca attcgcgcgt
     7021 cccgcagcgc agaccgtttt cgctcgggaa gacgtacggg gtatacatgt ctgacaatgg
     7081 cagatcccag cggtcaaaac aggccgcagt aaggcggtcg ggatagtttt cttgcggccc
     7141 taatccgagc cagtttaccc gctctgcgac ctgcgccagc tggcaggtca ggccaatccg
     7201 tgccggatgc ggtgtatcgc tcgctacttc aacatcaacg gtaatcgcca tttgaccact
     7261 accatcaatc cggtaggttt tccggctgat aaataaggtt tttccctgat gctgccacgc
     7321 gtgagcggtc gtaatcagca ccgcgtcggc aagtgtatct gccgagcact gcaacaacgc
     7381 tgcttcggcc tggtaatggc ccgccgcctt ccagcgttcg acccaggcgt tagggtcaat
     7441 gcgggtcgct tcacttacgc caatgtcgtt atccagcggt gcacgggtga actgatcgcg
     7501 cagcggcgtc agcagttgtt tttcatcgcc aatccacatc tgtgaaagaa ggcctgactg
     7561 acggttaaat tgccaacgct tattacccag ctcgatgcaa aaatccgttt cgctggtggt
     7621 tagttgcggg atgatatggg acgcggaggg gagcgtcaca ctgaggtttt ccgccagacg
     7681 ccactgctgc caggcgctga tgtgcccggc ttctgaccat gcggtcgcgt tcggttgcac
     7741 tacgcgtact gtgagccaga gttgcccggc gctctccggc tgcggtagtt caggcagttc
     7801 aatcaactgt ttaccttgtg gagcgacatc cagaggcact tcaccgcttg ccagcggctt
     7861 accatccagc gccaccatcc agtgcaggag ctcgttatcg ctatgacgga acaggtattc
     7921 gctggtcact tcgatggttt gcccggataa acggaactgg aaaaactgct gctggtgttt
     7981 tgcttccgtc agcgctggat gcggcgtgcg gtcggcaaag accagaccgt tcatacagaa
     8041 ctggcgatcg ttcggcgtat cgccaaaatc accgccgtaa gccgaccacg ggttgccgtt
     8101 ttcatcatat ttaatcagcg actgatccac ccagtcccag acgaagccgc cctgtaaacg
     8161 gggatactga cgaaacgcct gccagtattt agcgaaaccg ccaagactgt tacccatcgc
     8221 gtgggcgtat tcgcaaagga tcagcgggcg cgtctctcca ggtagcgaaa gccatttttt
     8281 gatggaccat ttcggcacag ccgggaaggg ctggtcttca tccacgcgcg cgtacatcgg
     8341 gcaaataata tcggtggccg tggtgtcggc tccgccgcct tcatactgca ccgggcggga
     8401 aggatcgaca gatttgatcc agcgatacag cgcgtcgtga ttagcgccgt ggcctgattc
     8461 attccccagc gaccagatga tcacactcgg gtgattacga tcgcgctgca ccattcgcgt
     8521 tacgcgttcg ctcatcgccg gtagccagcg cggatcatcg gtcagacgat tcattggcac
     8581 catgccgtgg gtttcaatat tggcttcatc taccacatac aggccgtagc ggtcgcacag
     8641 cgtgtaccac agcggatggt tcggataatg cgaacagcgc acggcgttaa agttgttctg
     8701 cttcatcagc aggatatcct gcaccatcgt ctgctcatcc atgacctgac catgcagagg
     8761 atgatgctcg tgacggttaa cgccgcgaat cagcaacggc ttgccgttca gcagcagcag
     8821 accattttca atccgcacct cgcggaaacc gacatcgcag gcttctgctt caatcagcgt
     8881 gccgtcggcg gtgtgcagtt caaccaccgc acgatagaga ttcgggattt cggcgctcca
     8941 cagtttcggg ttttcgacgt tcagacgtag tgtgacgcga tcggcataac caccacgctc
     9001 atcgataatt tcaccgccga aaggcgcggt gccgctggcg acctgcgttt caccctgcca
     9061 caaagaaacc gtcacccgca gctcatcgcg cagctcgcca tacatctgaa cttctgcttc
     9121 cagtaccgcg cggctgaaat catcattaaa gtgagtggca acatggaaat cgctgatttg
     9181 tgtgctcggt ttgtgcagca acgagacgtc acggaaaatg ccgctcatcc gccacatatc
     9241 ctgatcttcc agataactgc cgtcactcca gcgcagcacc atcaccgcga ggcggttttc
     9301 tccggcgcgt aaaaatgtgc tcaggtcaaa ttcagacggc aaacggctgt cctggccgta
     9361 accgacccag cgcccgttgc accacagatg aaacgccgag ttaacaccat caaaaataat
     9421 tcgcgtctgg ccttcctgta gccagctttc atcaatatta aatgtgagcg agtaacaacc
     9481 cgtcggattc tccgcgggaa caaacggcgg attgaccgta atgggatagg tcacgttggt
     9541 gtagatgggc gcgtcgtaac cgtgcatctg ccagtttgag gggacgatga cagtatcggc
     9601 gtcaggaaga tcgcactcca gccagctttc cggcaccgct tctggcgccg gaaaccagac
     9661 aaattgccac tcaccattca ggctgcgcaa ctgcggggag gggcgaacag tgcgggcttc
     9721 ttcgctattg cgccagctgg cgaaaggggg atgtgccgca aggcgattaa gttgggtaac
     9781 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaatccg taatcatggt
     9841 catagctgtt tcctgtgtga aattgttatc cgctcacaat ttcacacaac atacgagccg
     9901 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9961 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    10021 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
    10081 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
    10141 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
    10201 atataacacg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg
    10261 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
    10321 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
    10381 atggcactcc agtcgccctc ccgttccgct atcggctgaa tttgattgcg tgtgagatat
    10441 ttatgccagc ccgccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
    10501 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagccgcgt accgtcttca
    10561 tgggagaaaa taatactgtt gatgggagtc tggtcagaga catcaagaaa taacgccgga
    10621 acattagcgc aggctgcttc cacagcagtg gcatcctggt catccagcgg atagttaatg
    10681 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
    10741 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
    10801 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
    10861 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
    10921 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
    10981 acgcgagaaa cggtctgata agagacaccg gcatactctg cgacatcgta tagcgttact
    11041 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccacga
    11101 aaggttttgc gccattcgat ggtgtcaacg taaatgcacg ccgcttcgcc gtccggccac
    11161 cagaatagcc tgcgattcaa ccccttcttc gatctgtttt gctacccgtt gtagcgccgg
    11221 aagatgcttt tccgctgcct gttcaatggt cattgcgctc gccatataca ccagattcag
    11281 acagccaatc acccgttgtt cactgcgcag cggtacggcg atagaggcga tcttctcctc
    11341 ctgatcccag ccgcggtagt tctgtccgta accctctttg cgcgcgcgcg ccagaatggc
    11401 ttgcagcttt aacggttctc gtgccagttg atagtcatca ccggggcggg cggctaacat
    11461 ttcgattaat tccttgcgtt cttgttccgg gcaaaaggcc agccaggtca ggcccgaggc
    11521 ggttttcagg agcggtaaac gtcgcccgac cattgcccgg tgaaaggata agcggctgaa
    11581 acggtgggtg gtttcgcgta ccaccattgc atcaacatcc agggtggaca catctgtcgg
    11641 ccataccact tcgcgcaaca gatcgcccag cagcggggcc gccagtgcag aaatccactg
    11701 ttcgtcacga aatccttcgc ttaattgccg cactttgatg gtcagacgaa aactgtcatc
    11761 ggaggggcta cggcggacat atccctcttc ctgcagcgtc tccagcagtc gccgcacagt
    11821 ggtgcgatgc aggccgctga gttccgccag cagcccgacg ctggcaccgc catcaagttt
    11881 atttaacata tttaataaca ttagaccgcg ggttaagccg cgcacggttt tgtattccgt
    11941 ctgctcattg ttctgcatat taattgacat ttctatagtt aaaacaacgt tgtgcacctg
    12001 gtgcacattc gggcatgttt tgattgtagc cgaaaatacc cttcctatac tgagcgcaca
    12061 ataaaaaatc atttacatgt ttttaacaaa ataagttgcg ctgtactgtg cgcgcaacgg
    12121 cattttgtcc gagtcgtgag gtactgaaat ggcaatacaa caccctgaca tccagcctgc
    12181 tgttaaccat agcgttcagg tggcgatcgc tggtgccggt ccggttgggc tgatgatggc
    12241 gaactatctc ggtcagatgg gcattgacgt gctggtggtg gagaaactcg ataagttgat
    12301 cgactacccg cgtgcgattg gtattgatga cgaggcgctg cgcaccatgc agtcggtcgg
    12361 cctggtcgag aatgttctgc cgcacactac accgtggcac gcgatgcgtt ttctcacccc
    12421 aaaaggccgc tgttttgctg atattcagcc aatgaccgat gaatttggct ggccgcgccg
    12481 taacgccttt attcagccac aggtcgatgc ggtgatgctg gaagggttgt cgcgttttcc
    12541 gaatgtgcgc tgcttgtttg cccgcgagct ggaggccttc agccagcaaa atgacgaagt
    12601 gaccttgcac ctgaaaacgg cagaagggca gcgggaaacg gtcaaagccc agtggctggt
    12661 agcctgtgac ggtggagcaa gttttgtccg tcgcactctg aatgtgccgt ttgaaggtaa
    12721 aactgcgcca aatcagtgga ttgtggtaga tatcgccaac gatccgttaa gtacgccgca
    12781 tatctatttg tgttgcgatc cggtgcgccc gtatgtttct gccgcgctgc ctcatgcggt
    12841 acgtcgcttt gaatttatgg tgatgccggg agaaaccgaa gagcagctgc gtgagccgca
    12901 aaatatgcgc aagctgttaa gcaaagtgct gcctaatccg gacaatgttg aattgattcg
    12961 ccagcgtgtc tacacccaca acgcgcgact ggcgcaacgt ttccgtattg atcgcgtact
    13021 gctggcgggc gatgccgcgc acatcatgcc ggtatggcag gggcagggct ataacagtgg
    13081 tatgcgcgac gcctttaacc tcgcatggaa actggcgttg gttatccagg ggaaagcccg
    13141 cgatgcgctg ctcgatacct atcaacaaga acgtcgcgat cacgccaaag cgatgattga
    13201 cctgtccgtg acggcgggca acgtgctggc tccgccgaaa cgctggcagg gtacgttacg
    13261 tgacggcgtt tcctggctgt tgaattatct gccgccagta aaacgctact tcctcgaaat
    13321 gcgcttcaag ccgatgccgc aatattacgg cggtgcgctg atgcgtgagg gcgaagcgaa
    13381 gcactctccg gtcggcaaga tgtttattca gccgaaagtc acgctggaaa acggcgacgt
    13441 gacgctgctc gataacgcga tcggcgcgaa cttcgcggta attggctggg gatgcaatcc
    13501 actgtggggg atgagcgacg agcaaatcca gcagtggcgc gcgttgggca cacgcttcat
    13561 tcaggtggtg ccggaagtgc aaattcatac cgcacaggat aaccacgacg gcgtgctacg
    13621 cgtgggcgat acgcaaggtc gtctgcgtag ctggttcgcg caacacaatg cttcgctggt
    13681 ggtgatgcgc ccggatcgct ttgttgccgc caccgccatt ccgcaaaccc tgggcaagac
    13741 cctgaataaa ctggcgtcgg tgatgacgct gacccgccct gatgccgacg tttctgtcga
    13801 aaaggtagcc tgatatgcac gcttatcttc actgtctttc ccactcgccg ctggtggggt
    13861 atgtcgaccc ggcgcaagag gtgctcgatg aggtcaatgg cgtgattgcc agcgcccgcg
    13921 agcgtattgc ggctttctcc cctgaactgg tggtgctgtt tgcgcctgat cactacaacg
    13981 gctttttcta tgacgtgatg ccaccgttct gtttaggcgt tggagcgacg gcaattggtg
    14041 atttcggcag tgcggcagga gagctgcccg tgcctgtgga gctggcggag gcctgtgcgc
    14101 atgccgtcat gaagagcggg atcg
//
LOCUS       DAEVZA010000128        14002 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEVZA010000128 DAEVZA010000000
VERSION     DAEVZA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.128"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1404
                     /gene="leuA"
                     /locus_tag="LAF92_003933"
     CDS             <1..1404
                     /gene="leuA"
                     /locus_tag="LAF92_003933"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBI9792860.1"
                     /translation="SVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFI
                     ATSPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAA
                     INAGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLA
                     AVHAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVS
                     QICNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRS
                     GRAAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEH
                     FRLDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVK
                     YSLTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVE
                     KELQRKAQHNENNKETV"
     gene            1404..2495
                     /gene="leuB"
                     /locus_tag="LAF92_003934"
     CDS             1404..2495
                     /gene="leuB"
                     /locus_tag="LAF92_003934"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBI9792861.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            2498..3898
                     /gene="leuC"
                     /locus_tag="LAF92_003935"
     CDS             2498..3898
                     /gene="leuC"
                     /locus_tag="LAF92_003935"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBI9792862.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            3909..4514
                     /gene="leuD"
                     /locus_tag="LAF92_003936"
     CDS             3909..4514
                     /gene="leuD"
                     /locus_tag="LAF92_003936"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBI9792863.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(4563..5741)
                     /gene="setA"
                     /locus_tag="LAF92_003937"
     CDS             complement(4563..5741)
                     /gene="setA"
                     /locus_tag="LAF92_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBI9792864.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(5843..5974)
                     /gene="sgrT"
                     /locus_tag="LAF92_003938"
     CDS             complement(5843..5974)
                     /gene="sgrT"
                     /locus_tag="LAF92_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBI9792865.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6063..7718
                     /gene="sgrR"
                     /locus_tag="LAF92_003939"
     CDS             6063..7718
                     /gene="sgrR"
                     /locus_tag="LAF92_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBI9792866.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      7752..7851
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7882..8865
                     /gene="thiB"
                     /locus_tag="LAF92_003940"
     CDS             7882..8865
                     /gene="thiB"
                     /locus_tag="LAF92_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBI9792867.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            8841..10451
                     /gene="thiP"
                     /locus_tag="LAF92_003941"
     CDS             8841..10451
                     /gene="thiP"
                     /locus_tag="LAF92_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBI9792868.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRSWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10435..11133
                     /gene="thiQ"
                     /locus_tag="LAF92_003942"
     CDS             10435..11133
                     /gene="thiQ"
                     /locus_tag="LAF92_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBI9792869.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11247..12011)
                     /locus_tag="LAF92_003943"
     CDS             complement(11247..12011)
                     /locus_tag="LAF92_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI9792870.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12097..12975)
                     /gene="araC"
                     /locus_tag="LAF92_003944"
     CDS             complement(12097..12975)
                     /gene="araC"
                     /locus_tag="LAF92_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBI9792871.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13314..>14002
                     /locus_tag="LAF92_003945"
     CDS             13314..>14002
                     /locus_tag="LAF92_003945"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="catalyzes the phosphorylation of ribulose to
                     ribulose 5-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBI9792872.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPIL"
BASE COUNT         3361 a         3842 c         3718 g         3081 t
ORIGIN      
        1 tcggtgcaaa ccatcgcccg ccaggttaaa aacagccgcg tatgtgcgtt agctcgctgc
       61 gtggagaaag atatcgacgt ggcggccgaa tccctgaaag tcgccgaagc cttccgtatt
      121 cataccttta ttgccacttc gccaatgcac atcgccacca agctgcgcag cacgctggat
      181 gaagtgatcg aacgcgctat ctatatggtg aaacgcgccc gtaattacac cgatgatgtt
      241 gaattttctt gcgaagatgc cgggcgtaca cccattgccg atctggcgcg agtggtcgaa
      301 gcggcgatta atgccggtgc caccaccatc aacattccgg acaccgtggg ctacaccatg
      361 ccgtttgagt tcgccggaat catcagcggc ctgtatgaac gcgtacctaa catcgacaaa
      421 gccattattt ccgtacatac ccacgacgat ttaggcctgg cggtcggcaa ctcactggcg
      481 gcggtacatg ccggtgcacg ccaggtggaa ggtgcaatga acgggatcgg cgagcgtgcc
      541 ggtaactgtt cgctggaaga agtcatcatg gcgattaaag ttcgtaagga tattctcaac
      601 gtccacaccg ccattaatca ccaggagata tggcgcacca gccagttagt tagccagatt
      661 tgtaatatgc cgatcccggc aaacaaagcc attgttggca gcggcgcatt cgctcactcc
      721 tccggtatcc accaggatgg cgtgctgaaa aaccgcgaaa actacgaaat catgacacca
      781 gaatctattg gtctgaacca aatccagctg aatctgacct ctcgttcggg gcgtgcggcg
      841 gtgaaacatc gcatggatga gatggggtat aaagaaagtg aatataattt agacaatttg
      901 tacgatgctt tcctgaagct ggcggacaaa aaaggtcagg tgtttgatta cgatctggag
      961 gcgctggcct tcatcggtaa gcagcaagaa gagccggagc atttccgtct ggattacttc
     1021 agcgtgcagt ctggctctaa cgatatcgcc accgccgccg tcaaactggc ctgtggcgaa
     1081 gaagtcaaag cagaagccgc caacggtaac ggtccggtcg atgccgtcta tcaggcgata
     1141 aaccgcatca ctgactataa cgtcgaactg gtgaaataca gcctgactgc taaaggtcac
     1201 ggtaaagatg ctctgggtca ggtggatatt gtcgccaact acaacggtcg ccgcttccac
     1261 ggcgtcggcc tggccaccga tattgtcgag tcctccgcca aagccatggt gcacgtactg
     1321 aacaatatct ggcgcgccgc agaagtcgaa aaagagttgc aacgcaaagc tcaacacaac
     1381 gaaaacaaca aggaaaccgt gtgatgtcga agaattacca tattgccgta ttgccggggg
     1441 acggtattgg tccggaagtg atgacccagg cgctgaaagt gctggatgcc gtgcgcaacc
     1501 gctttgcgat gcgcatcacc accagccatt acgatgtagg cggcgcagcc attgataacc
     1561 acgggcaacc actgccgcct gcgacggttg aaggttgtga gcaagccgat gccgtgctgt
     1621 ttggctcggt aggcggtccg aaatgggaac atttaccacc agaccagcaa ccagaacgcg
     1681 gcgcgctgtt gcctttgcgt aagcacttca aattattcag caacctgcgc ccggcaaaac
     1741 tgtatcaggg gctggaagca ttctgtccgc tgcgtgcaga cattgccgct aacggcttcg
     1801 acatcctgtg cgtgcgcgaa ctgaccggcg gcatctattt cggtcagcca aaaggccgcg
     1861 aaggtagcgg acaatatgaa aaagcgtttg ataccgaggt gtatcaccgt tttgagatcg
     1921 agcgtattgc ccgcatcgcg tttgaatctg ctcgtaaacg tcgccacaaa gtcacctcaa
     1981 tcgacaaagc taacgtgctg caatcctcta ttttatggcg tgaaatcgtc aacgagatcg
     2041 ccacggaata cccggatgtc gaactggcgc atatgtacat cgacaacgcc accatgcagc
     2101 tgattaaaga tccatcacag tttgacgttc tgctgtgctc caacctgttt ggcgacattc
     2161 tgtctgacga gtgcgcaatg atcactggct cgatggggat gttgccttcc gccagcctga
     2221 acgagcaagg ttttggtctg tatgaaccgg caggcggctc agcaccagat atcgcaggca
     2281 aaaacatcgc caacccgatt gcgcaaattc tgtcgctggc actgctgctg cgctacagcc
     2341 tggatgccga tgatgcggct tgcgccattg aacgcgccat taaccgcgca ttagaagaag
     2401 gcattcgcac cggggattta gcccgtggcg ctgccgccgt tagtaccgat gaaatgggcg
     2461 atatcattgc ccgctatgtg gcagaagggg tgtaatcatg gctaaaacgt tatacgaaaa
     2521 attattcgac gctcacgtag tgtacgaagc cgaaaacgaa accccgctgt tatatatcga
     2581 ccgccacctg gtgcatgaag tgacctcacc gcaggcgttt gatggtctgc gcgcccacgg
     2641 tcgcccggta cgtcagccgg gcaaaacctt cgccactatg gatcacaacg tctctaccca
     2701 gacgaaagac attaatgcct gtggtgaaat ggcgcgcatc cagatgcagg agctgattaa
     2761 aaactgcaaa gaatttggcg tcgagctgta tgacctgaat cacccgtatc aggggatcgt
     2821 ccacgtaatg gggccggaac agggcgtcac cttgccgggg atgaccattg tctgcggcga
     2881 ctcgcatacc gccacccacg gcgcgtttgg cgcactggcc tttggtatcg gcacttccga
     2941 agttgaacac gtactggcaa cacaaaccct gaaacagggc cgtgcgaaga ccatgaaaat
     3001 tgaagtccag ggcaaagccg cgccgggcat tacagcaaaa gatatcgtgc tggcaattat
     3061 cggtaaaact ggcagcgcag gcggcaccgg gcatgtggtg gagttttgcg gcgaagcaat
     3121 tcgtgattta agcatggaag gtcgtatgac cctgtgcaat atggcaatcg aaatgggcgc
     3181 aaaagccggt ctggttgcac cggacgaaac cacctttaac tatgtcaaag gccgtttgca
     3241 tgcgccgaaa ggcaaagatt tcgacgacgc cgttgcctac tggaaaaccc tgcaaaccga
     3301 cgaaggcgca actttcgata ccgttgtcac tctgcaagca gaagaaattt caccgcaggt
     3361 cacctggggc accaatccag gccaggtgat ttccgtgaac gacaatattc ccgatccggc
     3421 ttcgtttgcc gatccggttg aacgcgcgtc ggcagaaaaa gcgctggcct atatggggct
     3481 gaaaccgggc attccgctga ccgaagtggc tatcgacaaa gtgtttatcg gttcctgtac
     3541 caactcgcgc attgaagatt tacgcgcggc agcggagatc gccaaagggc gaaaagtcgc
     3601 gccaggcgtg caggcactgg tggttcccgg ctctggcccg gtaaaagccc aggcggaagc
     3661 ggaaggtctg gataaaatct ttattgaagc cggttttgaa tggcgcttgc ctggctgctc
     3721 aatgtgtctg gcgatgaaca acgaccgtct gaatccgggc gaacgttgtg catccaccag
     3781 caaccgtaac tttgaaggcc gccaggggcg cggcgggcgc acgcatctgg tcagcccggc
     3841 aatggctgcc gctgctgctg tgaccggaca tttcgccgac attcgcaaca ttaaataagg
     3901 agcacaccat ggcagagaaa tttatcaaac acacaggcct ggtggttccg ctggatgccg
     3961 ccaatgtcga taccgatgca atcatcccga aacagttttt gcagaaagtg acccgtacgg
     4021 gttttggcgc gcatctgttt aacgactggc gttttctcga tgaaaaaggc caacagccaa
     4081 acccggactt cgtgctgaac ttcccgcagt atcagggcgc ttccattttg ctggcacgag
     4141 aaaacttcgg ctgtggctcc tcgcgtgagc acgcgccctg ggcattgacc gactacggtt
     4201 ttaaagtggt gattgcgccg agttttgctg acatcttcta cggcaatagc tttaacaacc
     4261 agctgctgcc ggtgaaatta agcgatgcag aagtggacga actgtttgcg ctggtgaaag
     4321 ccaatccggg gatccatttc gacgtggatc tggaagcgca agaggtgaaa gcgggagaga
     4381 aaacctatcg ctttaccatc gatgccttcc gccgccactg catgatgaac ggtctggaca
     4441 gtattgggct taccttgcag cacgacgacg ccattgccgc ttatgaagca aaacaacctg
     4501 cgtttatgaa ttaatcccct tgcccggtca aatgaccggg ctttccgcta tcgtccacgt
     4561 catcaaacgt ctttaacctt tgcggttaaa aataatgcga caacagaaat aaccgcaatt
     4621 acccagtaga cagcaaagtg cccccaactt tgtgcaattg ctccctgaat aacgccagcc
     4681 agaattaccc cggtagaaat actgttagta aataaggtgg tagctgcccc cgctcttcca
     4741 ggcattaaat cctgaaacca tagcatccca atacccgcaa caatgccgat aaatacagcg
     4801 ttaaaaagtt gcagcgtcat caacgccata cggctatgaa agaaaatcaa tccggtgtaa
     4861 aacagtactc ctgccgccac tgctatgacc atcattcgcc gcttaccata acgtttgaca
     4921 tagtagccag ccagaatcat tgctggtatt tccagtccag ctgccgtccc catcaggaaa
     4981 cccgccagtt tgtctggcaa tcctaactcg ctactgatcc acaacggcat atcaataatg
     5041 tacatggtgt tgcaggtcca cattaacgtc gaggcgacaa ataacatccg tacgttacta
     5101 tcctgccagc caccttgcat tgataaagca ttttccgacg gcagttctac ccgcgccaca
     5161 gacggaagca taaatgcaat caataccaga ctgagtgtga atatcccggc ggcaatcgaa
     5221 aacatcaccg taaagccgta attcaacgcc agcataaagg ccaacggtgg accgataacc
     5281 catgccagag aaagctgcgc acgcatcacc gagctaaaca tcaccacttc tcgcgccgag
     5341 ttatccgcat attcccgcgc cagagcaaat aactgtggca ttgccgtatt ggccagagat
     5401 gccagaagca caccacaggt gataagcgtc agataatgac gattaaatgc aaacaatagc
     5461 gcattgccga tagccatcaa acagcaaaat ataatcagtt ttcgccgatc gccctgactg
     5521 tcagaacgtt ttgccaacca gaggcttacg ccgatcccag caatagcatt caccgtataa
     5581 aagagtccga tccagaaagg ttgcgcgcca acctcacgac tcagaaataa gctcaatgta
     5641 ggagcctgta gcgccccggc cacccccatc ataaaagcga ccagcataaa tgccgcgtaa
     5701 acaccgttca tacggcgagc catcgtcatt atccagatca tacgttccct ttttagcgcg
     5761 gcgagaataa aaaaaaccag caggtataat ctgctggcgg gtgattttac accaatactc
     5821 agtcacacat gatgcaggca agtcaacttt cagaattgcg gtcatcccac tgcatcagtt
     5881 cttcaagcat ggttaatcgt tgtgggacgc ttaaccaacg caaccagcac aacttcgctg
     5941 tcgcggtaaa atagtgctga taaaactgac gcatggggca cctccttgct tcatcgttag
     6001 gaattattga cttaatatag ggaaaataaa attgctgtct tttgcgcagg agttcccctt
     6061 ttatgccatc tgctcgtctg caacaacagt tcatccgcct gtggcaatgc tgcgagggta
     6121 aatcgcagga cacaacgctc aacgaactgg cagcgttatt gagctgctcg cgtcgtcata
     6181 tgcgtaccct gctcaacacc atgcaggatc gcggctggct gacgtgggaa gcggaagtcg
     6241 ggcgcggtaa acgctcgcgt ctgacattcc tctataccgg gctggcgctt cagcaacagc
     6301 gggcggaaga cctgctggag caggatcgta tcgaccaact agtgcagttg gttggcgaca
     6361 aagcgactgt gcggcaaatg ctggtttctc atctgggccg cagcttccgc caggggcggc
     6421 acatcctgcg cgtgctctac tatcgtccgt tgcgtaatct gctacctggc agcgcattgc
     6481 gccgttccga aacccatatt gcccggcaaa tcttcagttc gctaacgcgc ataaatgagg
     6541 aaaatgggga actggaagca gacatcgccc accactggca gcaaatatca ccgcttcact
     6601 ggcgtttctt tttgcgtcca ggagtccatt ttcatcatgg tcgtgaactg gaaatggacg
     6661 acgtgatcgc ctctttaaaa cgaatcaata cgctgccgct ctattcgcat attgcggaca
     6721 ttgtgtcgcc gacgccctgg acgctggata tccatctcac gcaaccggac cgctggttac
     6781 cgttactgct gggacaagtt ccggcgatga tcctgccgcg cgaatgggaa accctcagta
     6841 actttgccag ccatcccatc ggcaccggtc cgtatgcggt gatccgcaac accaccaatc
     6901 aactgaaaat tcaggcattc gatgacttct tcggttaccg ggcattaatc gatgaagtta
     6961 acgtctgggt tctgccggaa attgccgacg agccagctgg aggtctgatg ctaaaaggtc
     7021 cacagggcga ggaaaaagag attgaaagcc gcctggagga aggttgctac tatttactgt
     7081 tcgacagccg cacccatcgc ggggcgaatc agcaagtcag ggactgggta agctatgtgc
     7141 tttctccaac taatctggtc tatttcgctg aggaacagta ccagcaactg tggttcccgg
     7201 cttatggact gctcccccgt tggcaccatg cccgcaccat aaagagcgaa aaaccggctg
     7261 gcctggaaag cctcaccctg accttttatc aggatcacag tgagcatcgg gtgattgccg
     7321 ggatcatgca gcagattctg gcaagtcacc aggtcacgct ggaaatcaaa gagatcagct
     7381 acgatcagtg gcatgaagga gagatcgaga gcgatatctg gcttaatagc gccaacttta
     7441 cgctgccgct ggatttttcg ctgttcgcgc acttgtgcga ggtgccgctg ctccaacact
     7501 gtattccgat cgactggcaa gccgacgccg cccgctggcg caatggcgaa atgaacctgg
     7561 cgaactggtg ccagcaactg gtcgccagta aagcaatggt gccgcttatc caccactggc
     7621 tgatcattca gggacaacgc agtatgcgcg gcctgcgtat gaataccctc ggctggttcg
     7681 attttaaatc agcgtggttt gcgccgccgg atccatgatt gctggtagat aacaaaatca
     7741 ctacactaac gccgttctca acggggtgcc acgcaaacgc gtgcgctgag aaaatacccg
     7801 tcgaacctga tccggataac gccggcgaag ggatttgagg ctccttctca agtcctttgc
     7861 cactcttttt tgaggtgcaa agtgttaaaa aaatgtcttc ccctgctgtt gctgtgcaca
     7921 gcgcccgttt tcgctaaacc cgttctgact gtttatacct acgattcctt cgccgccgac
     7981 tgggggcctg gtccaaagat caaaaaagct tttgaagccg actgtaactg cgaactgaaa
     8041 ctggtggcgc tggaagatgg cgtttcgctt ctcaaccgtc tacggatgga aggcaaaaat
     8101 agtaaagccg atgtggtgct ggggctggat aacaatctgt tagacgccgc cagtaaaacc
     8161 ggactatttg ccaaaagcgg tgtggcagcg gatgccgtta acgttcccgg cggctggaat
     8221 aatgacactt tcgtcccgtt tgattacggc tatttcgcct tcgtctatga caagaacaaa
     8281 ctgaaaaacc cgccgcaaag cctgaaagaa ttggttgaga gcgatcaaaa ctggcgggtg
     8341 atttatcagg atccgcgcac cagtacgccg gggctgggcc tgctgttgtg gatgcaaaaa
     8401 gtctatggcg atgacgcccc acaagcctgg caaaaactgg cgaagaaaac cgtcacggtc
     8461 accaaaggct ggagcgaagc ctacggcctg tttttaaaag gtgaaagcga tctggtactg
     8521 agttacacca cctctccggc ttatcacatt ctcgaagaga aaaaagataa ctacgccgcc
     8581 gcgaacttta gcgaaggtca ctatctgcag gtggaagtcg ccgcccgcac cgctgccagc
     8641 aagcagccgg agctggcgca aaaattcctc cagtttatgg tttctccggc ttttcagaat
     8701 gcgatcccaa ccggtaactg gatgtatccg gtggcaaatg tcacgctgcc tgccgggttt
     8761 gaacaattga ccaaaccggc caccacgctg gagttcacac cagctgaagt ggctgcacaa
     8821 cgtcaggcat ggattagcga atggcaacgc gccgtcagcc gttaattccc ggctggttaa
     8881 ttccaggtgt aagcgccgcc acgctggtgg tagcggttgc gctcgcggcg tttctcgccc
     8941 tgtggtggaa cgcgccgcag ggtaactggg tggcagtctg gcaggacagc tacctgtggc
     9001 atgtggtgcg cttctccttc tggcaggcgt ttctctcggc gctactctct gtcgtacccg
     9061 cgatattcct cgcccgcgcc ctctatcgca ggcgctttcc gggtcggctg gcgctgttgc
     9121 gtctgtgcgc aatgaccttg atcctcccgg tgctagttgc tgttttcggc attcttagcg
     9181 tctatggtcg tcagggctgg ctggcatcgc tctgccaatc gctcggtctg gagtggacct
     9241 tttcgcccta cggcctgcaa ggtattttgc tggcgcacgt attttttaat ctgccgatgg
     9301 cgagccgctt attactccag gcactggaaa acatccccgg cgaacagcgt caacttgccg
     9361 cccagcttgg gatgcgtagc tggcattttt tccgcttcgt cgaatggccg tggttacggc
     9421 gacaaatccc gccggttgcc gcgcttatct ttatgctctg tttcgccagc ttcgccaccg
     9481 tgttgtcgct gggcggcggt ccgcaagcga cgactatcga gctggctatt tatcaggcgc
     9541 tgagttacga ctacgatccc gcccgcgcgg cgatgctggc gctgatccag atggtgtgct
     9601 gtctcggtct ggtgctgctg agtcagcgat tgagtaaggc cattgcgcct ggcaccacgc
     9661 tgctgcaagg ttggcgcgac ccggacgatc gtctgcatag ccgcatttgc gacacggtgt
     9721 taattgtgct ggcgctgctg ctgttgctgc caccgttact ggcggtgatc gtcgatgggg
     9781 taaatcgcca gttgccggaa gtgctggcac aaccggtgct gtggcaggcg ctgtggacct
     9841 cgttgcgtat tgcgctggcg gcaggtgtat tgtgcgtagt gctgaccatg atgctgctat
     9901 ggagcagtcg cgaactgcgg gcgcggcaga aaatgctggc gggtcaggcg ctggagatga
     9961 gcggcatgtt gatcctcgcc atgccgggga ttgtgctggc taccggcttc tttttactgc
    10021 tcaacaacac tatcggcctg ccacaatctg ctgacggcat tgtgattttc accaatgcgt
    10081 taatggcgat cccttatgcg ctgaaagtgc tggaaaaccc gatgcgcgat atcaccgccc
    10141 gctacagtat gttgtgtcag tcgctgggca ttgaaggctg gtcgcgctta aaagtggtcg
    10201 agctgcgcgc cctgaaacgt ccactggcgc aggcgctggc ttttgcctgc gtgctgtcga
    10261 ttggtgattt tggcgtagtg gcgttgttcg gtaacgatga tttccgtacc ctgccgtttt
    10321 atctctacca gcaaattggc tcctatcgca gccaggacgg cgcggtcacc gcgttaattc
    10381 tgctgctgct ctgttttctg ctgtttaccg tgattgaaaa actaccgggg cgaaatgtta
    10441 aaactgactg atatcacctg gctttatcac catttgccga tgcgttttag cttaacggta
    10501 gaacgcggcg agcaggtggc aatcctcggg ccaagcggcg cgggtaaaag taccctgctg
    10561 aatttgatcg ccggttttct gacgccagcc agcggttcgc tgactatcga cggcgttgat
    10621 cacaccacga cgccgccgtc acgccgtccg gtatcgatgc tgtttcagga aaacaacctg
    10681 ttcagtcacc tgacggtcgc gcagaacatc gggctggggc ttaatccggg attgaagctg
    10741 aacgcggcgc agcaggagaa aatgcacgct atcgcccgcc agatggggat tgataattta
    10801 atggcgcggt taccgggcga gctttccggc ggtcagcgac agcgcgtggc gttagcgcgt
    10861 tgtctggtgc gcgaacagcc gattttattg ctcgatgaac cgttctctgc gctcgatccg
    10921 gcgttacgtc aggagatgct gacgctggtg agcacgagct gccagcagca aaaaatgacg
    10981 ctattgatgg tgtcgcacag tgtggaagat gcggcgcgga tcgccacacg ctcggtggtg
    11041 gtcgccgacg ggcgcatcgc ctggcagggt aagaccaatg agttgttgag cggtaaggcg
    11101 agtgcttcgg cactattggg gattacgggt tagtatgcac tgtcggatgc ggcgtgaacg
    11161 ccttatccga cctacgggta gtgcgttttg taggcctgat aagacgcggt cagcgtcgca
    11221 tcaggcaacc cgcacaagac tatctcctaa cccccaacca ctttacgcaa aatatcgata
    11281 tacaccggca tcaacgggtg gcgaattaac accaccagcg ccaccacgcc gatggcagaa
    11341 attaacggcg tcagccacaa caaacggccg cgcgacaaat aatgacttaa acgatcggtt
    11401 gctttaccgc tgcgccataa ccgccagcac agccagccac caacccacaa aaataccgcc
    11461 gtcgccagca gcaaccattt aaactcgccg ctctgcattc cggctggaat atcgatcgcc
    11521 gcgcccgcca gaatcccagg caggaagtaa aacggtggcc acagcaggca gccgataata
    11581 ttcggcgtaa tgaattttgc caccggcaga tccagcattc ccgccaccat tggcaccagc
    11641 ggacgcgtcg ggccaacaaa gcgaccgacc agaatggtga acatgctgtg ttgatgcaac
    11701 gcatgttcag ttttatcgag taatgctttg tttttcttca gaaatgacca gcgatgcaac
    11761 ggctttttaa aacgccagcc cagccagaag gagatccagt cgcccagcag gcaaccgaca
    11821 atccctgcca gccaggcgtg ccagaaactt aactcgccgc tgccaatcag cgctcccagc
    11881 cccgccatca gcaccgtacc gggtagaatc aaaccgacca gcgccagcga ctccagaaag
    11941 gccaccaaca ccaccgccat caatgaatac acggtagatt gggtaataaa gtgttccagc
    12001 aatgcttgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta
    12061 ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc
    12121 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
    12181 aaatactcgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
    12241 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
    12301 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggtgacaa
    12361 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
    12421 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
    12481 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
    12541 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
    12601 cgcttcatcc gggcgaaaga aacccgtatt ggcaaatatt gacggccagt taagccattc
    12661 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
    12721 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
    12781 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
    12841 ataacctttc atccccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
    12901 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
    12961 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
    13021 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa cccaaccggt
    13081 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
    13141 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
    13201 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
    13261 atcgcaactc tctactgttt ctccataccc gtttttttgg atggagtgaa acgatggcga
    13321 ttgcaattgg cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg gactgcgcca
    13381 gcggtgaaga gatcgccacc agcgtagagt ggtatccccg ttggcaaaaa gggcaatttt
    13441 gtgatgcccc gaataaccag ttccgtcatc atccgcgtga ctacattgag tcaatggaag
    13501 cggcactgaa aaccgtgctt gcagagctta gcgtcgaaca gcgcgcagct gtggtcggga
    13561 ttggcgttga cagtaccggc tcgacgcccg caccgattga tgccgacggt aacgtgctgg
    13621 cgctgcgccc ggagtttgcc gaaaacccaa acgcgatgtt cgtattgtgg aaagaccaca
    13681 ctgcggttga agaagcggaa gagattaccc gtttgtgcca cgcgccgggc aacgttgact
    13741 actcccgcta cattggtggt atttattcca gcgaatggtt ctgggcaaaa atcctgcatg
    13801 tgactcgcca ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag ctgtgcgact
    13861 gggtgccagc tctgctttcc ggtaccaccc gcccgcagga tattcgtcgc ggacgttgca
    13921 gcgccgggca taaatctctg tggcacgaaa gctggggcgg cttgccgcca gccagtttct
    13981 ttgatgagct ggacccgatc ct
//
LOCUS       DAEVZA010000129        13814 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEVZA010000129 DAEVZA010000000
VERSION     DAEVZA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.129"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..174
                     /locus_tag="LAF92_003946"
     CDS             <1..174
                     /locus_tag="LAF92_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792873.1"
                     /translation="LVPSDDKVGPFGAKSISEIGVNGAAPAIATAIHDACGIWLREWH
                     FTPEKILTALEKI"
     gene            339..1739
                     /gene="xanQ"
                     /locus_tag="LAF92_003947"
     CDS             339..1739
                     /gene="xanQ"
                     /locus_tag="LAF92_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HBI9792874.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            1757..3073
                     /gene="guaD"
                     /locus_tag="LAF92_003948"
     CDS             1757..3073
                     /gene="guaD"
                     /locus_tag="LAF92_003948"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HBI9792875.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            3109..4476
                     /gene="ghxQ"
                     /locus_tag="LAF92_003949"
                     /gene_synonym="ygfQ"
     CDS             3109..4476
                     /gene="ghxQ"
                     /locus_tag="LAF92_003949"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HBI9792876.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVTGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(4512..5000)
                     /locus_tag="LAF92_003950"
     CDS             complement(4512..5000)
                     /locus_tag="LAF92_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBI9792877.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCELREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(5000..6919)
                     /gene="ygfT"
                     /locus_tag="LAF92_003951"
     CDS             complement(5000..6919)
                     /gene="ygfT"
                     /locus_tag="LAF92_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBI9792878.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQGKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            7355..8803
                     /gene="uacT"
                     /locus_tag="LAF92_003952"
     CDS             7355..8803
                     /gene="uacT"
                     /locus_tag="LAF92_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBI9792879.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            8805..8930
                     /locus_tag="LAF92_003953"
     CDS             8805..8930
                     /locus_tag="LAF92_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792880.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(8927..8998)
                     /locus_tag="LAF92_003954"
     CDS             complement(8927..8998)
                     /locus_tag="LAF92_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792881.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            9053..9601
                     /gene="idi"
                     /locus_tag="LAF92_003955"
     CDS             9053..9601
                     /gene="idi"
                     /locus_tag="LAF92_003955"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBI9792882.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(9644..11161)
                     /gene="lysS"
                     /locus_tag="LAF92_003956"
     CDS             complement(9644..11161)
                     /gene="lysS"
                     /locus_tag="LAF92_003956"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBI9792883.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(11171..12269)
                     /gene="prfB"
                     /locus_tag="LAF92_003957"
     CDS             complement(join(11171..12193,12195..12269))
                     /gene="prfB"
                     /locus_tag="LAF92_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBI9792884.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(12360..>13814)
                     /gene="recJ"
                     /locus_tag="LAF92_003958"
     CDS             complement(12360..>13814)
                     /gene="recJ"
                     /locus_tag="LAF92_003958"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBI9792885.1"
                     /translation="AMRSLGCSNIDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVD
                     NGISSHAGVEHARSLGIPVIVTDHHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVA
                     FYLMLALRTFLRDQGWFDERGIAIPNLAELLDLVALGTVADVVPLDANNRILTWQGMS
                     RIRAGKCRPGIKALLEVANRDAQKLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDN
                     IGEARVLANELDALNQTRKEIEQGMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGV
                     VGILASRIKERFHRPVIAFAPAGDGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFG
                     GHAMAAGLSLEEDKFELFQQRFGELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLL
                     RDAGPWGQMFPEPLFDGHFRLLQQRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWP
                     DNGVREVQLAYKLDINEFRGNRSLQIIIDNIWPI"
BASE COUNT         3151 a         3593 c         3467 g         3603 t
ORIGIN      
        1 ttggtgccga gcgacgataa agtcggcccg ttcggggcga aatcgatctc ggaaatcggt
       61 gtaaatggcg cagctccggc gattgctacc gcaattcacg atgcctgcgg catctggtta
      121 cgcgaatggc atttcacacc ggagaaaata ctcaccgcgc tggaaaaaat ataagtaata
      181 aatggccgct tcggcggcct gtttctctgt ttgaacgatg actaaatagt gactgcgaaa
      241 atgagataag ccgttctcaa ggcgtcctgc gccccaaatt cgtgggaatt tattttacta
      301 attcagatga tcaaatttac tttaaaagga gtgaagggat gtctgatata aacaatgcag
      361 gttctgacct tatatttgaa ctggaggatc gccctccctt tcatcaggct ctcgttggtg
      421 ccattaccca tctgttggca attttcgttc cgatggtaac ccccgcgtta atcgtggggg
      481 cggccttaca gctttccgct gaaacaactg cctatcttgt ttctatggcg atgatcgcct
      541 ctggtattgg tacctggtta caagtaaacc gctacggcat cgtcggttct ggcctactct
      601 caattcagtc agtcaatttt tcatttgtta cggtcatgat tgcgctgggc agcagcatga
      661 aaagcgacgg ttttcacgaa gagttaatca tgtcgtcgct tctcggcgtc tccttcgttg
      721 gcgcatttct ggttgtcgga tcttcattta tcttgcccta tttacgtcgg gttattacgc
      781 ctaccgtcag cggtattgtg gtactgatga tcggcttaag cctgattaaa gtcggcatta
      841 tcgattttgg tggaggattt gcagccaaaa gcagcggtac attcggcaat tacgaacatc
      901 tcggcgttgg tttattggtt ttaattgtgg tgatcggctt taactgctgt cgcagtccgt
      961 tgctacgcat gggagggatc gccattgggc tatgtgtcgg ctatatcgca tcgttatgcc
     1021 tgggcatggt ggatttcagc agtatgcgca atttgccgtt aatcaccatc ccgcatccgt
     1081 tcaaatacgg ctttagtttt agcttccatc agttcctggt ggttggcacg atttatctgc
     1141 ttagcgtgct ggaagctgtc ggcgatatca ccgccacggc aatggtttcc cgccgaccca
     1201 ttcagggaga agagtatcag tcccgactga aaggcggcgt gctggcagac ggtctggttt
     1261 ctgttatcgc ctccgctgtc gggtcattac ccttaaccac gtttgcgcaa aataatgggg
     1321 ttattcagat gactggcgtc gcttcacgtt atgtcgggcg aaccatcgcg gtaatgctgg
     1381 ttatcctcgg tttgtttccg atgattggcg gcttcttcac gaccattccc tcggcagttc
     1441 tgggaggcgc aatgacgttg atgttttcca tgattgccat cgctgggatt cgcataatca
     1501 tcaccaacgg tttaaagcgc cgagaaacac ttattgtcgc cacttcttta ggtttaggac
     1561 ttggcgtctc ctacgatccc gaaattttta aaatattgcc agcctctatt tatgtactag
     1621 ttgaaaaccc tatttgtgct ggcgggttaa ctgcgatttt attaaatatt atcctccctg
     1681 gtggctaccg acaggaaaac gttctgcctg gtattacctc agcggaagag atggattaac
     1741 agtaaaggag tcaatgatgt caggagaaca cacgttgaaa gcggtacgag gcagttttat
     1801 tgatgtcacc cgtacggtcg ataacccgga agagattgcc tctgcgctgc ggtttattga
     1861 ggatggttta ttactcatta aacagggaaa agtggaatgg tttggcgaat gggaagacgg
     1921 aaagcatcaa attcctgaca ctattcgcgt gcgcgactat cgcggcaaac tgatagtacc
     1981 gggctttgtc gatacacata tccattatcc gcaaagtgaa atggtggggg cctatggtga
     2041 acaattgctg gagtggttga ataaacacac cttccctact gaacgtcgtt atgaggattt
     2101 agagtacgcc cgcgaaatgt cggcattctt catcaagcag cttttacgta acggaaccac
     2161 cacggcgctg gtgtttggca ctgttcatcc gcaatctgtt gatgcgctgt ttgaagccgc
     2221 cagtcatatc aatatgcgta tgattgccgg taaggtgatg atggaccgca acgcaccgga
     2281 ttatctgctc gacactgccg aaagcagcta tcaccaaagc aaagaactga ttgaacgctg
     2341 gcacaaaaat ggtcgtctgc tatatgcgat tacgccacgc ttcgccccga cctcatctcc
     2401 tgaacagatg gcgatggcgc aacgcctgaa agaagaatat ccggatacgt gggtacatac
     2461 ccatctctgt gaaaacaaag atgaaattgc ctgggtgaaa tcgctttatc ctgaccatga
     2521 tggttatctg gatgtttacc atcagtacgg cctgaccggt aaaaactgtg tctttgctca
     2581 ctgcgtccat ctcgaagaaa aagagtggga tcgtctcagc gaaaccaaat ccagcattgc
     2641 tttctgtccg acctccaacc tttacctcgg cagcggctta ttcaacttga aaaaagcatg
     2701 gcagaagaaa gttaaagtgg gcatgggaac ggatatcggt gccggaacca ctttcaacat
     2761 gctgcaaacg ctgaacgaag cctacaaagt attgcaatta caaggctatc gcctctcggc
     2821 atatgaagcg ttttacctgg ccacgctcgg cggagcgaaa tctctgggcc ttgacgattt
     2881 gattggcaac tttttacctg gcaaagaggc tgatttcgtg gtgatggaac ccaccgccac
     2941 tccgctacag cagctgcgct atgacaactc tgtttcttta gtcgacaaat tgttcgtgat
     3001 gatgacgttg ggcgatgacc gttcgatcta ccgcacctac gttgatggtc gtctggtgta
     3061 cgaacgcaac taataataaa actttaacat cctcgtgagg acatcattat gtctggagac
     3121 atcctacaaa caccggacgc accaaagcca cagggcgcgc tggataatta ttttaaaatt
     3181 accgctcgtg gcagtaccgt tcgtcaggaa gtactggctg gcttaacgac ctttctggcc
     3241 atggtttatt ccgttatcgt cgttccggga atgctgggca aagcaggttt tcctcccgca
     3301 gctgtgtttg ttgccacctg tctggtcacg ggcttcggct cgttgctgat ggggttgtgg
     3361 gccaatttgc caatggcgat tggttgtgcg atttccttga cggcatttac cgcattcagt
     3421 ctggtactcg ggcaacaaat tagcgttcct gtcgcactgg gcgcggtctt tctgatgggc
     3481 gtcatcttca ccgctatttc cgtaaccggt gtgcgtacct ggatcttacg taatttaccg
     3541 atgggtatcg ctcacggtac aggtatcggt atcggcctgt ttctgctgct gattgctgct
     3601 aacggtgtgg gtatggttat caaaaacccg attgaaggct tgccagtggc gctcggtgcg
     3661 tttacctcct tcccggtgat gatgagcttg ctggggctgg cggtcatctt cggtctggag
     3721 aagtgccgcg tacccggcgg gatcttgttg gtgattatcg caatttcgat tatcggctta
     3781 atctttgacc cagcggtgaa ataccacggt ctggtggcga tgccaagtct gactggcgaa
     3841 gatggtaagt ctctgatttt cagcctcgat attatgggcg cactccagcc aaccgtactt
     3901 ccgagtgtac tggcattggt gatgaccgca gtgttcgacg ccactggcac catccgtgcc
     3961 gtcgccggtc aggcgaattt gttggataaa gacaaccaga tcatcaacgg cggcaaagcc
     4021 ctgaccagtg actcagtaag ttcaatattc tccggtctgg tgggcgcagc gcccgcagcg
     4081 gtttatatcg aatcagcggc aggaaccgcc gccgggggta aaacagggtt gaccgcaacc
     4141 gtagtggggg cgttattcct gctgattctg ttcttatcac cgctgtcatt tttgatccct
     4201 ggttacgcca ctgcacccgc tctgatgtac gtgggtttgc tgatgttaag taacgtctcg
     4261 aagctggatt tcaatgattt tattgacgct atggctggcc tggtgtgtgc cgtgttcatc
     4321 gttctgactt gtaatatcgt taccggtatt atgctgggct ttgtgacact ggtcgtaggc
     4381 cgcgtctttg cacgcgaatg gcaaaagctg aatattggta cggtgatcat tactgccgca
     4441 ctggtcgcat tttacgcggg tggttgggca atctaataga ttctccgcgc ctttcagcgc
     4501 ggagatttct ttcacagagg attctcaccg ctggcaacaa tacgctgctg gcggactcgc
     4561 ctgagttctc tttcagtaag taactgcagc gcctgtgttg ggcaagattc aacgcaggct
     4621 ggcccctctt cccgcagttc acagagatcg catttcacga tttgttgccg ggtatccccc
     4681 ggcaaagact gaatggtgat cattccaaac gggcacgcac tgacgcaact ctgacagcca
     4741 atacaacggg cagaattggt ctgcaccacc tgctcgccca tcgtcagcgc ccccacgggg
     4801 caagcgccaa cacaaggggc gttttcacac tgatggcaca tcaccggcgc actgatgctg
     4861 tccagtcgct gtaccttcag ccggggtaaa aagacatcgg cattcaactc ctgttctgaa
     4921 gggtgagcga ccacacaggc tacttcacaa gtacggcagc caatacagtc agccggatta
     4981 acgataatta acgatttcat cacgatgcct tcgtatcaaa cagagttaac atatcgcgcg
     5041 ccgcctgcct tcctgcggcc attgcagtga caaccagatc cgcgccatga actgcatcac
     5101 caccagcaaa gacttttttc agatgcgtct gggtaggtaa atacccgacg tcaccggttt
     5161 gaatcaggcc ccatttatcg agtttaattc cgctgccctg caaccacggc atggcatgcg
     5221 cctggaaacc aaaggccata atcagaacat cggcgggcaa ttcaaactct gaacccgcta
     5281 ccggacgagg acgacggcga ccatccggcc ccggctcacc catggcggta cgaatcaggc
     5341 ccaccgcagt taaacgccca tcttcgtcac aagcgatata ttgcggttga acattgaact
     5401 gaaactcgac accttcctcg cgcgcattaa ccacctcttt gcgcgagccc ggcatactga
     5461 cttcatcacg acgatacgcg caggtcacgc tggcagcatt gaggcgaatg gaagtccgca
     5521 aacaatccat tgtcgtatcg ccaccgccca ataccacgac tcgcttacct tccacgtccg
     5581 tcagcggata ctcttcagac tccggcaatc ccatgagctg gcgggtatgg gcagtcagga
     5641 acggtagagc ctgaatgaca ccgggtgcat cttcatgcgg cagatctgct cgcatcatcc
     5701 cgtaagtccc cacgccgatg aaaactgcat catattcaga agttaaatcg ctaaaggtaa
     5761 tatcgcggcc aatttcacag ttaagatgaa agtcgattcc cattgcggtg aatatctctc
     5821 gccgctggct taataccgtt ttatcgagtt tgaaaggagg aatgccaaaa gtcagcatac
     5881 cgccaatttc tggatggcga tcaaagacat caacctgaac tcccgcgcgc gccagaatat
     5941 cagcacaccc taaccctgct ggcccagcgc caatcaccgc cactttttcg ctacggggaa
     6001 caactttgct gacatcggga cgccagccca tcgccagcgc ggtatcggtg atgtagcgtt
     6061 ccagattacc gatagagact gcgccagagt gatctttcaa agtacatgcg ccttcacaaa
     6121 gacggtcctg tgggcatacc ctgccgcaga tttctggtaa ggaactggtc tggtggcaaa
     6181 gttctgccgc ttcaataatc tttccttcct gtaccagacg gatgtaatcc ggaatagcgt
     6241 tatgcagcgg gcaatgccag ttgcagttag ctttttcggc acaataaaca cagcggtcac
     6301 tctcataagt cgcttgcagt ggatccagcc cgcaatagat ttcgccaaag tgggttttcc
     6361 gttcactcgc tgaaatttta tctgcgcctt tacgcgagtt aacggggagc aacgctgcac
     6421 tgcgagatgg ctgagcgtct gatgacgctt ttcctgctgc cgttttgccc tggcgagcca
     6481 cctttatcca ctgtaaccct ttatcgtcca tcagtcgtaa cgcctgtgtt gggcagactt
     6541 caatacaggc ttgcgtgccg gaactgcgct ggttacaaag gtcgcatttc tgcgcaatcg
     6601 tatcgaccat ctcaacgacg ccaaaggggc aagcgattgc gcatctttta cagccaatac
     6661 atttttgctc gtccagttgt acgctatcgg actggaaagt cagagcatta accggacaag
     6721 ccgtaacgca aggggcattg ttgcagtgat ggcaggccac cggattcgca gcctggcctt
     6781 tccctacaac gtggatacgc ggtcgaaagt cactgtgact cagcggccag ttttcttgat
     6841 tgtgtgccac cgcacaggca atttcacaag catgacagcc tatacattcc gcagcttcag
     6901 cagcgataaa cttattcatt tgcatccctt tcatttaatg agttatgtct tctcaaattt
     6961 cgcgatgcaa taaacgggca tatattaggt agcatgaccg ttttaattat aattttgcga
     7021 ctgagttcaa attattgccc ctcaatctat gccgctttca ttatttcaac attgccgacc
     7081 aaaagagcgc tatccagata aaaacgatta aatgcgagag tgcgacatgc cagaatgatt
     7141 aatgaaatat caatatgaga aaattacaga taattgcatt gtcaggttct cttctcattt
     7201 tcgtggttta accacgatta tgtgattgcc tccgcaattc ctgtctctaa ctccccttcc
     7261 tcgcaaaaac tggcactcca cgagcatgtg tttagacagt ttcattaacg taaacggttg
     7321 ctttttactc tggcgggcga aaggagaaac actgatgagc gccatagatt cccaacttcc
     7381 ctcatcttct gggcaagacc gcccaactga tgaggttgac cgcatattat caccaggaaa
     7441 gctgatcata ctcggtctgc aacacgtcct tgtcatgtac gcaggtgcag tcgctgttcc
     7501 tcttatgatt ggtgaccgac tgggcctctc aaaagaagct attgcgatgc tcattagctc
     7561 ggatctcttt tgctgcggga tcgtcacatt attgcaatgt atcggtatcg gccgctttat
     7621 ggggatccgc ctgccggtga ttatgtcggt gacctttgct gctgtaacac caatgatagc
     7681 cattgggatg aacccggata tcggcctgct ggggatattt ggtgccacta tcgccgcggg
     7741 ttttatcacc acattattag caccacttat cggtcgcttg atgcctttat tcccgccact
     7801 ggttaccggt gtggttatta cttctatcgg gcttagcatc attcaggtgg gtattgactg
     7861 ggccgccgga ggtaaaggga atccgcaata tggtaatccc gtttatttag gtatctcctt
     7921 tgctgtctta atttttatct tgctcattac tcgctatgcg aaaggattta tgtccaacgt
     7981 cgccgtatta ctggggattg tatttggctt tttactttcg tggatgatga atgaagtcaa
     8041 tttatccggg ctacatgatg cttcatggtt tgcgattgtc acgccgatgt catttggtat
     8101 gccgattttc gatcccgttt ccattctgac catgactgcc gtgttaatca tcgtgtttat
     8161 cgagtcgatg gggatgttcc tggcactggg tgaaatagtc ggtcgtaaac tctcttcaca
     8221 cgatattatt cgcgggctgc gtgtcgatgg cgtagggaca atgataggcg gaacgtttaa
     8281 cagcttcccc cacacgtcat tttcacaaaa cgttggcctg gttagcgtga cgcgcgttca
     8341 tagccgctgg gtgtgtattt cttcgggaat tatattaatc ctgtttggca tggtgccaaa
     8401 aatggcggtg ctggtcgcct ccattccgca atttgtgctg ggcggtgctg ggctggtgat
     8461 gttcggcatg gtactggcga cagggattcg aattctgtcg cgctgtaact acaccaccaa
     8521 ccgttacaac ctctatattg tggcgatcag tctcggcgtt ggcatgactc cgacgctctc
     8581 tcacgatttc ttttctaagt taccggccgt actgcaaccg ttgctgcata gcggcattat
     8641 gctcgcaacc cttagcgccg ttgtgctgaa tgtcttcttt aatggctacc agcatcatgc
     8701 tgacctggtg aaggaatccg tctctgataa agatttaaaa gtcaggacag tacgtatgtg
     8761 gcttctgatg cgcaagctga agaaaaatga gcatggagaa taatatgaat tttttaatgc
     8821 gcgctatatt cagtctgctg ttgcttttta ctctctctat tcctgtcatt tctgactgtg
     8881 ttgcaatggc cattgaaagt cgcttcaaat atatgatgct acttttttaa atggttttta
     8941 cctgtcggca tccgctcaaa acgggcggtt gtcgataaac gctcacttgg ttaatcattt
     9001 cactcttcaa ttatctataa tgatgagtga tcagaattac atgtgagaaa ttatgcaaac
     9061 ggaacacgtc attttattga atgcacaggg agttcccacg ggtacgctgg aaaagtatgc
     9121 cgcacacacg gcagacaccc gcttacatct cgcgttctcc agttggctgt ttaatgccaa
     9181 aggacaatta ttagttaccc gccgcgcact gagcaaaaaa gcgtggcctg gcgtgtggac
     9241 taactcggtt tgtgggcacc cacaactggg agaaagcaac gaagacgcgg tgatccgccg
     9301 ttgccgttat gagcttggcg tggaaattac gcctcctgaa tctatctatc ctgactttcg
     9361 ctatcgcgcc accgatccga atggcattgt ggaaaatgaa gtgtgtccgg tatttgccgc
     9421 acgcaccacc agtgcgttac agatcaacga tgatgaagtg atggattatc aatggtgtga
     9481 tttagcagat gtattacacg gtattgatgc cacaccgtgg gcgttcagtc cgtggatggt
     9541 gatgcaggca gccaatagtg aagcaagaaa attgttgtct gctttcgcgc agcacaatta
     9601 aaaaaccccg acatttgccg gggttgtgag cataacgtaa tgcttatttt accggacgca
     9661 tcgccgggaa cagaataacg tcgcggatgg tatggctgtt ggtgaacagc attaccatac
     9721 ggtcgatgcc aatccccaga cctgctgtcg gcggtaagcc atgttccagt gcggtaacgt
     9781 aatcttcatc gtagaacatc gcttcgtcgt cacctgcgtc tttcgcggca acctgatcca
     9841 ggaagcgttg cgcctgatct tccgcgtcat tcagctcgct aaagccgtta ccgatttcac
     9901 gaccaccgat gaagaactca aagcggtctg tgatttccgg gttaatgtcg ttacgacgcg
     9961 ccagcggaga aacttctgcc ggatattcag taatgaaggt cggctgaatc agatgtgctt
    10021 ctgccacttc ttcgaagatc tcggtaacga tacggcccag accccagctc ttctcaacgt
    10081 ggataccgat agattcagca atcgctttcg cagagtcgaa gttatccaga tccgccatgt
    10141 cggtttccgg gcgatatttc ttgatcgctt cacgcatggt cagtttttcg aacggtttac
    10201 cgaagtccag cgtcacgtcg ccgtaggtca cttccgtctt accgagaata tcctgtgcca
    10261 gagtacggaa cagcgattcg gtcagctcga tcagatcttt gtaatctgcg taagccatgt
    10321 agagttccat catggtgaac tctgggttat gacgtacgga gataccttcg ttacggaagt
    10381 tacggttgat ttcgaataca cgctcgaagc caccgaccac cagacgcttg aggtacagtt
    10441 ccggcgcgat acgcaggtac atgtcgagat ccagcgcgtt atggtgggtg ataaacggac
    10501 gcgcagcggc accgccaggg atcacctgca tcatcggcgt ttcaacttcc ataaagccgc
    10561 ggttcaccat gaactggcga ataccagaga ggatctgcga gcgtacttta aaggtgttac
    10621 gggattcatc gttagagatg agatccaaat aacgctgacg atagcgcgct tcctgatcct
    10681 gcaagccgtg gaatttatcc ggcagcggac gcagtgcttt ggtcagcaga cgcagctcgg
    10741 tacagtggat agacagttcg ccggttttgg ttttgaacag tttacctttc gcgccgagga
    10801 tgtcgccgag gtcccatttt ttgaactgct cgttgtaaac gccttccggc agatcgtcac
    10861 gggcaacgta cagctgaatg cgaccgccaa cgtcctgcag ggtaacgaaa gacgctttac
    10921 ccataatacg acgggtcatc atgcggccag caacggcgac ttcgatgttc agcgcttcca
    10981 gttcttcgtt ctctttaccg tcgaattctg cgtgcaattg gtcagaggta tgatcgcgac
    11041 ggaaatcgtt cgggaaggca atcccctgct cgcgcaggtt cgccagcttc tcacgacgcg
    11101 ttttcagttc attgttaaga tcgactaccg cgtcagcgcc ctgtgcgtgt tgttcagaca
    11161 tgttggttcc tcataaccct gctttcaaac ttgcttcgat aaattgatcc aggctgccgt
    11221 ccagcacggc ctgcgtgttg cgggtttcta ccccggtacg cagatcttta atgcgggagt
    11281 catcaaggac ataagaacga atctggctgc cccagccgat gtcggatttg ttatcttcca
    11341 tcgcctgttt ctcggcattt ttcttctgca tctccagttc ataaagcttc gctttcatct
    11401 gcttcatggc ctggtctttg ttcttgtgct gggaacggtc gttctggcat tgggtcacga
    11461 tcccggttgg gatgtgggta atacgcaccg cagattcggt acggttaacg tgctgaccgc
    11521 ccgcgccgga cgcgcgataa acgtcaatgc gcagatccgc cgggttgatt tcgatatcaa
    11581 tatcatcatc aacttccggg taaacaaacg cagagctgaa cgatgtgtgg cgacgaccgc
    11641 cggagtcaaa cgggctttta cgcaccaggc ggtgaacgcc agtttctgta cgcagccagc
    11701 cgtaagcgta atcgccggag attttgatcg tcacggattt aatacccgcc acttcacctt
    11761 ccgactcttc gatgatttca gttttgaaac cacgcgattc tgcccagcgc agatacatac
    11821 gctcaagcat gctcgcccag tcctgtgctt ccgtaccgcc agaccccgcc tgaatatcga
    11881 ggtagcagtc ggcgctgtca tattcgccag agaacatacg gcggaactca agctgcgcca
    11941 gtttttcttc cagggcgtcg agttcagcaa cggcttcgtt aaaggtttct tcgtcgtcag
    12001 cttctacagc cagttccagc agaccagaaa catcttccag cccctgtttc atttggtcga
    12061 gggtgtcgac aacggcttcg agggaggaac gctctttacc cagcgcctgt gcgcgttcgg
    12121 gttcgttcca gacatccggc tgttccagct cggcgtttac ttcttccaga cgctctttct
    12181 tggcgtcgta gtcaaagata ccccctaaga acgtcggagc gttccgtgag gtcctgaatg
    12241 cgattattta ccggattaat ttcaaacatg gtctgatttc ttttattgag ctagtcaaaa
    12301 tgcggtgata agagcgggat tgtacccaat ccacgctctt ttttatagag aagatgacgc
    12361 taaattggcc agatattgtc gatgataatt tgcaggctgc ggttgccgcg aaactcgttg
    12421 atatcgagct tgtaagccag ttgcacttcg cgcacgccgt tatccggcca gagggcggta
    12481 tcgacattaa aagcaatacc atccagcagc ggaccgccgc cgaccggttc gaccatgact
    12541 ttcaaatgac gttcgcccac cagccgctgt tgcagcagac ggaaatgacc atcaaacagc
    12601 ggctccggga acatctgccc ccacgggcca gcatcgcgca gcagctgcgc cacttccatg
    12661 gtcatttcgg ccgggcttaa cgggccgtct gacaccactt cgccttgcaa tagcgaaggg
    12721 tccagccact cggtaaccag ctcgccaaac cgttgttgaa agagttcgaa tttatcctct
    12781 tccagcgaca aacccgccgc catcgcatga ccgccaaact tcagcatcat accggggtag
    12841 agtgtgtcta atcgctccag cgcatcacgc atatgcagcc cctgaatgga gcgaccggaa
    12901 cctttcagcg taccatcacc tgctggcgca aaggcgataa ccggacggtg aaaacgctct
    12961 ttgatgcgcg aagccagaat accgacaacg ccctgatgcc attcggggtg atacattgcc
    13021 agcccgccgg gtagcgtgtc gcgacttcgc tccagtttct cgcacagggt caaggcttca
    13081 atctgcattc cttgttcgat ctcttttcgc gtctgattta gcgcatcgag ttcatttgcc
    13141 agcacgcgcg cttcgccgat gttgtcgcac agcaagagcg ccacaccgac ggacatatcg
    13201 tccagtcgcc cggcagcatt gagacgtggc cccagcgcaa aacctaaatc gctggcggcg
    13261 agtttttgcg catcgcgatt tgccacttcc agcagcgctt taatccccgg acggcacttt
    13321 ccggcacgga tgcgactcat cccctgccag gtcagaatgc gattattagc gtccagcggc
    13381 acgacgtccg ctactgtccc cagcgctacc agatccagca gttctgccag gttaggaatt
    13441 gcgatgccgc gctcatcaaa ccagccctga tcgcgcaaaa aggtgcgcag cgccagcatc
    13501 agataaaacg ccacacccac gcctgccagt gatttcgacg ggaaattaca gtcgcgcaag
    13561 ttagggttaa tgatcgcttc cgctgcgggt aatgtttcgc ccggcaaatg gtgatcggta
    13621 acaataaccg gaatgcccaa cgagcgagcg tgttcaacgc ccgcatggga ggaaataccg
    13681 ttatccaccg tgacaattaa ctgcgcgcca cgggcatggg cctgatcgac tacttccggg
    13741 cttaagccgt aaccgtcttc gaaacggttt ggtaccagat agtcgatatt gctgcaacca
    13801 agcgagcgca tcgc
//
LOCUS       DAEVZA010000130        13755 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEVZA010000130 DAEVZA010000000
VERSION     DAEVZA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.130"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..2271
                     /gene="dosP"
                     /locus_tag="LAF92_003959"
                     /gene_synonym="pdeO"
     CDS             <1..2271
                     /gene="dosP"
                     /locus_tag="LAF92_003959"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HBI9792886.1"
                     /translation="LWGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGM
                     SRELQLEKKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHL
                     DRPVIVLDPERHIVQCNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLW
                     KTARDQDEFLLLTRTGEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNI
                     LAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSW
                     SATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQ
                     FDPMTGLPNRNNLHNYLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVN
                     RFREKLKPDQYLCRIEGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTL
                     SIGISYDVGKNRDYLLSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEA
                     ISNNQLKLVYQPQIFAETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGR
                     WVIAEACRQLAEWRSQNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVE
                     ITESMMMEHDTEIFKRIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVD
                     RCLTEKRILALLEAITSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLP
                     AEEIPGWMSSVLPLKI"
     gene            2529..3110
                     /gene="ddpX"
                     /locus_tag="LAF92_003960"
     CDS             2529..3110
                     /gene="ddpX"
                     /locus_tag="LAF92_003960"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HBI9792887.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            3124..4674
                     /locus_tag="LAF92_003961"
     CDS             3124..4674
                     /locus_tag="LAF92_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI9792888.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQHKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            4676..5698
                     /locus_tag="LAF92_003962"
     CDS             4676..5698
                     /locus_tag="LAF92_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9792889.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            5695..6591
                     /locus_tag="LAF92_003963"
     CDS             5695..6591
                     /locus_tag="LAF92_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBI9792890.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6588..7574
                     /locus_tag="LAF92_003964"
     CDS             6588..7574
                     /locus_tag="LAF92_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI9792891.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQVLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            7567..8493
                     /locus_tag="LAF92_003965"
     CDS             7567..8493
                     /locus_tag="LAF92_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI9792892.1"
                     /translation="MSNTLLTLCDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(8549..8980)
                     /gene="osmC"
                     /locus_tag="LAF92_003966"
     CDS             complement(8549..8980)
                     /gene="osmC"
                     /locus_tag="LAF92_003966"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBI9792893.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            9326..9541
                     /gene="bdm"
                     /locus_tag="LAF92_003967"
     CDS             9326..9541
                     /gene="bdm"
                     /locus_tag="LAF92_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBI9792894.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            9643..9780
                     /gene="sra"
                     /locus_tag="LAF92_003968"
     CDS             9643..9780
                     /gene="sra"
                     /locus_tag="LAF92_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBI9792895.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            9937..11634
                     /gene="maeA"
                     /locus_tag="LAF92_003969"
     CDS             9937..11634
                     /gene="maeA"
                     /locus_tag="LAF92_003969"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBI9792896.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            11768..12778
                     /gene="adhP"
                     /locus_tag="LAF92_003970"
     CDS             11768..12778
                     /gene="adhP"
                     /locus_tag="LAF92_003970"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBI9792897.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKPGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            12925..13209
                     /locus_tag="LAF92_003971"
     CDS             12925..13209
                     /locus_tag="LAF92_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBI9792898.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(13260..>13755)
                     /gene="fdnI"
                     /locus_tag="LAF92_003972"
     CDS             complement(13260..>13755)
                     /gene="fdnI"
                     /locus_tag="LAF92_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBI9792899.1"
                     /translation="RILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLK
                     GNEHKVADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIH
                     AAAGIILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKES
                     EEGI"
BASE COUNT         3405 a         3462 c         3561 g         3327 t
ORIGIN      
        1 ctctgggggt acaaacgtga agaagtcatt ggcaataaca ttgatatgct gattccgcgg
       61 gatttgcgtc ctgcgcatcc tgaatacatt cgtcataacc gtgaaggcgg taaagcgcgt
      121 gtcgagggga tgagtcggga gctgcagctg gagaaaaaag acggcagtaa aatctggacc
      181 cgttttgcgc tatcgaaagt gagcgccgag gggaaagttt attacctggc gctggtacgg
      241 gatgccagcg tagaaatggc gcaaaaagaa cagactcgac aattgattat tgccgttgac
      301 catctcgacc gaccggtgat tgtcctcgat ccggaacgcc atattgtgca gtgcaatcgc
      361 gcatttaccg aaatgtttgg ttactgcatt agcgaagcca gcggtatgca gcccgataca
      421 ctcctgaaca ttcctgaatt ccctgccgat aaccgcattc gtttacaaca gttgctatgg
      481 aaaaccgccc gcgatcagga cgaatttctg ctgttgacgc gcaccggtga aaaaatctgg
      541 attaaagcct ctatcagccc ggtttatgac gtgctcgcgc atctgcagaa cctggtaatg
      601 actttctcgg atatcaccga agaacggcaa atccgccagc ttgaaggcaa tattctcgcc
      661 gccatgtgca gcagcccgcc atttcatgaa atgggggaaa tcatttgtcg taacatcgaa
      721 tctgttctca acgaatcgca tgtttcgctg ttcgcactgc gcaacgggat gccgatacac
      781 tgggcgtcat cttcgcacgg tgcagaagtt caaaatgcgc aaagctggtc agcgaccatt
      841 cgtcagcgtg atggcgcgcc tgcggggatc ctgcaaatta aaacctcgtc aggagcagaa
      901 accagcgcct ttatcgaacg cgtggcagat atcagtcagc atatggccgc gctggcgctg
      961 gaacaggaaa aaagccgcca gcatattgaa caactcatcc aatttgatcc gatgaccggt
     1021 ctgccaaatc gcaataacct gcacaattac ctcgatgacc tggtcgacaa agccgtctct
     1081 cccgtggtgt atctcatcgg tgttgaccat attcaggatg tgattgatag ccttggctat
     1141 gcgtgggccg atcaggcatt gctggaagtg gtcaatcgct ttcgtgaaaa actcaaaccg
     1201 gatcagtatc tctgtcgtat cgaaggtacg cagtttgtcc tcgtgagcct ggaaaacgac
     1261 gtcagtaaca ttacccagat cgccgatgag ctacggaatg tggtcagcaa gccgataatg
     1321 attgacgata aaccattccc gcttaccttg agtattggca tcagctacga cgtgggtaaa
     1381 aaccgcgatt acctactctc cactgctcac aatgcaatgg attatattcg caagaatggc
     1441 ggtaacggct ggcagttctt cagcccggcg atgaacgaaa tggtaaaaga gcgtttggtt
     1501 ttaggcgcag cgctgaaaga agcgattagc aataaccaac tgaaacttgt ttaccagccg
     1561 caaatcttcg cagaaacggg tgaactgtac ggcatcgaag cccttgcgcg ctggcacgat
     1621 ccccagcatg gtcatgtgcc cccttcacgg tttattcctc tcgcagaaga gattggtgaa
     1681 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     1741 agccagaata ttcatatccc ggcgctttcc gttaacttgt cggcactgca ctttcgcagt
     1801 aatcaactgc ctaatcaggt gtctgatgca atgcacgcct ggggtattga cggccaccag
     1861 ctgacggtag aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     1921 attcagatcc tgcgtgatat gggcgtgggc ttatcggtag atgattttgg tacgggcttt
     1981 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagtttt
     2041 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     2101 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     2161 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     2221 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     2281 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     2341 agctacgatt ccgggagatt ccttccttaa cttcccctct tgcaccaaaa ctgcactacg
     2401 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgcttc
     2461 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     2521 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtaatcttc cctgatctgg
     2581 agatcgaatt gaaatacgcc tgcgctgata acatcacagg taaagctatt tatcagcaag
     2641 cgcgttgtct gttacacaag gatgcgatta ccgcgctggc gaaaagtatc agcatcgccc
     2701 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     2761 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     2821 acagccgtgg cacggcgatc gacctgacgc ttcgtgatga gcacgggaac atcctcgata
     2881 tgggcgcagg gttcgatgaa atgcacgagc gctcccatgc ctatcaccct tccgtcccgc
     2941 ccgccgctca gcgcaatcgg ctgttgctga atgcgataat gactggtggc ggttttgtcg
     3001 gtatctccag cgaatggtgg cacttcgaat taccccaggc agcgagttac cctctgctcg
     3061 ctgatcaatt ctcttgtttt atatcccctg gcacacagca cgtcagttaa tcaggaaact
     3121 gtcatgaaga gatcgatatc gtttcgtccc acattgctcg cgctcgtcct tgccacaaat
     3181 ttcccggttg cgcacgccgc cgtaccaaaa gatatgctgg tgattggtaa ggccgccgat
     3241 ccacaaaccc tcgacccggc ggtaacaata gataataacg actggacagt gacctacccg
     3301 tcttatcagc ggctggttca gcacaaaacg gacggtgata aaggctcaac cgacgttgaa
     3361 ggcgatctgg caagtagctg gaaagcgtct gacgatcaaa aagagtggac gttcaccctg
     3421 aaagataatg ctaaatttgc cgatggcaca cctgtcactg ccgaagcagt aaaactttct
     3481 tttgagcggc tactaaaaat cggccagggg ccagcagaag catttcccaa agatttaaag
     3541 attgatgctc ccgacgaaca tacggtgaag tttaccctta gccaaccatt cgcaccgttc
     3601 ctctacacgc tggcgaatga cggtgcatcc attatcaatc cggcggtctt aaaggaacat
     3661 gcagcggatg atgctcgcgg cttcctcgcg caaaataccg ccggttccgg accatttatg
     3721 ctgaaaagct ggcaaaaagg tcagcaatta gttctggtgc caaatccgca ttacccaggc
     3781 aataaaccga acttcaaacg ggtatcggta aaaattatcg gtgaaagtgc ctcccgtcgc
     3841 ctgcagctct cccgtggcga cattgacatt gccgatgcgc tgccggtgga tcaactcaac
     3901 gccctgaagc aggaaaataa agtcaatgtg gcagagtatc cgtcactgcg cgttacctat
     3961 ctgtatctga ataacagcaa agcgcctctt aatcaggcgg atctgcgtcg ggccatttcc
     4021 tggtctaccg attatcaggg catggttaac ggcattctga gtggtaacgg aaaacagatg
     4081 cgcggcccga ttccggaagg catgtggggc tacgatgcga cggcaatgca atacaaccat
     4141 gacgaaacga aagccaaagc cgaatgggat aaagtgacga gcaaacccac cagcctgacg
     4201 tttctctact ccgataacga tccgaactgg gagcctattg ctttggcgac acaatccagt
     4261 ctcaacaagc tgggcatcaa tgtgaagctg gaaaagctgg cgaacgccac catgcgcgac
     4321 agagtgggta aaggtgatta cgacattgcg attggcaact ggagtccgga ttttgccgac
     4381 ccgtatatgt ttatgaatta ctggtttgag tcagacaaaa aaggtctgcc gggtaaccgc
     4441 tcgttctatg aaaacagtga ggtcgataag ttactgcgca atgcgcttgc gaccaccgac
     4501 cagacgcagc gtacccggga ctaccagcag gcacagaaaa tcgtcattga tgacgctgct
     4561 tatgtgtacc tgttccagaa aaactaccaa ctggcgatga acaaagaggt gaaaggcttt
     4621 gtgttcaatc ccatgctgga acaggtcttc aatatcaata ccatgagtaa ataacgtgac
     4681 cttctggagt attttacgcc aacgctgctg ggggctggtg ctcgtggtgg cgggcgtctg
     4741 cgtgattacg tttattatct cgcatctgat ccctggtgat ccggcgcggt tactggcggg
     4801 tgaccgcgcc agcgatgcta tcgtggaaaa tattcgccag caactgggac tggaccagcc
     4861 actgtatgta cagttttacc gctacgtcag cgacctgttt catggtgacc tgggaacatc
     4921 cattcgtacc gggcgtccgg tgctggaaga gttgcgtata tttttcccgg cgacgctgga
     4981 gctggctttt tgtgccctgc tgctggcact cttgattggc atcccgctgg gcatactctc
     5041 tgcagtctgg cgaaatcgct ggctggatca tctggtgcga ataatggcca ttaccggaat
     5101 ctccacacct gcgttctggc ttgggctggg cgtcattgtg ctgttttatg gtcatctaca
     5161 aatacttccc ggcggcggaa ggcttgatga ctggctggat ccaccaacgc acgttaccgg
     5221 cttttatctg ctcgatgcgt tgcttgaagg caacggtgaa gtcttcttca atgcgttgca
     5281 acatctcatc ttaccggcat taacgctggc gttcgttcac ctgggaattg tcgctcgcca
     5341 gatccgctca gcgatgctgg aacaattgag tgaagactac attcgtaccg cccgggccag
     5401 cggcttgccc ggctggtata tcgttttatg ttatgcgcta cccaatgcgt tgatcccatc
     5461 gattaccgta ttgggtttgg cgctgggcga tttgttgtat ggcgcagtgc tcaccgaaac
     5521 cgtttttgcc tggcccggaa tgggtgcatg ggtagtaaca tcaatacagg cgctcgactt
     5581 cccggcagtg atgggctttg ccgtcgtggt ttcatttgct tatgtgctgg tcaacctggt
     5641 ggtggatttg ctctatttgt ggattgatcc gcgtatcgga cgtggaggtg gtgaatgatg
     5701 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5761 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5821 atgctattga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     5881 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     5941 cgcgatctgt tcagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtc
     6001 gtggttgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6061 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6121 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6181 attgctattg tgcgaattcc cttttatgtg cgcctggcgc gggggcaagc attagttgta
     6241 cgccagtata cctatgttca ggcggcgaaa acctttggtg cgtcgcgttg gcatctgatc
     6301 agctggcata ttttacgtaa ctccctgccg ccgctgatcg tgcaggcatc gctggatatc
     6361 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6421 agtgctgaat ggggcgcgat ggtagcaaat ggtcgcaact atgtactcga tcagtggtgg
     6481 tattgcgcat ttccgggggc agcgattttg cttaccgccg ttgggtttaa tctctttggt
     6541 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6601 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6661 tcaacaatgt gtccttgcag attaaccgcg gtgaaattgt cggtctggtg ggagaatccg
     6721 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6781 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6841 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     6901 tcaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     6961 taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg caaatcccgg
     7021 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7081 taatgattgc gctggcattc tcctgcgagc cgcaattgat tattgccgac gaaccgacta
     7141 cggcgctgga cgtcacgcta cagttgcagg tactgcgttt gcttaaacat aaagcccgcg
     7201 ccagtggaac tgcggtactg ttcatcagcc atgatatggt cgtggtgtcg caactgtgcg
     7261 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgtgacg gcagacatta
     7321 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7381 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7441 aaggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
     7501 cgctgacagc gtgtggtgac aacaaccagc gctgcgcctg ttggtatccg cagcaggagg
     7561 tcataagtgt ctaacacgtt attaacgtta tgcgacgtcc atatcaattt cccggcccgt
     7621 aaaaactggc ttggtaaaac tacggaacat gttcatgcca ttaatggtat tgatttacag
     7681 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
     7741 gcacagcttt taatgggtat gctgcaaccg agccacgggc agtacatccg ttctggctca
     7801 caacgcatta tgcagatggt gtttcaggac ccgctctctt cgctcaatcc gcgcttaccg
     7861 gtgtggcgca tcatcacaga accgctctgg atagctaagc gtagtagtga acaacagcgg
     7921 cgagcgttag cagaggagtt ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
     7981 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
     8041 tcgcagcctg acgtgattgt gcttgatgag ccaacctctg cactggatat ctccgtgcag
     8101 gcgcagatcc tcaatttact ggtaacgcta caggtaaatc gcgggctgac ctatgtgctg
     8161 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
     8221 gggcagattg tggaattagg agacgcgcag caggtgttga cggcacctgc acatccatac
     8281 acccgattat tgctggattc cctccccgcc attgataaac cgctggagga agaatgggca
     8341 ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt tttctacgaa
     8401 cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattaacaat aagggaggac
     8461 ggacgtgaga tccggtgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
     8521 cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
     8581 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
     8641 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
     8701 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatc gatgttggcg
     8761 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
     8821 cgccaatcag ttcttcaggg ttggttcctt tttcgccttc aaaacgcgtg ttaaatccat
     8881 acggctgttg gttcagcacg ccactctcgg tggatactgt tcccttcccg cgtttgatat
     8941 cgccttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc ctgtgggctt
     9001 aaaaaggaaa ttatagacta cgtgagaaac agcacgagga taagcaggat attccgaata
     9061 aaaatccggc agaaatatta cttctgcact taaattaaat ttccacaata gataaccggg
     9121 aatatataac ccaagagata ttgcattaat attaatcaac tcaccacaat aataatacct
     9181 atcaatatca tgaggttaaa atataaaaat catcaaccag gactaatctt aacaacgaag
     9241 cggcaaatat ttgccatgct gaatgtgctt tataaatctg cgatccgtag cagacaccat
     9301 aaatacacag acacggagaa tcactatgtt tacttattat caggcagaaa attcaacagc
     9361 agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcacg gtgttgtcac
     9421 tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata acgacatcct
     9481 cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc ctacgcttta
     9541 aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc ttcgctacgg
     9601 cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc gaaccgtcag
     9661 gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat agttaccgaa
     9721 ggtgacaaat ccagcgtggt aaataatcca accggcagaa aacgccccgc tgaaaagtaa
     9781 ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat ggtgtttttg
     9841 tttttatctg ctctatactt ggggccgacg ccctggcggt aaagcaaaga cgataaaagc
     9901 gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac aaaaaaacag
     9961 cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt gttgaataaa
    10021 ggcagtgcct tcagcatgga agaacgccgt aacttcaatc tgctggggtt actgccggaa
    10081 gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca gggattcaaa
    10141 accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga aaccctcttc
    10201 taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac cccaaccgtc
    10261 ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt gtttatctct
    10321 taccagaacc ggcacaatat ggacgatatt ctgcaaaacg tgccgaacca taatattaaa
    10381 gtgattgtgg tgactgacgg tgaacgtatt ctagggcttg gtgaccaggg catcggcggg
    10441 atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat cagcccggcg
    10501 tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacagctgct taacgatccg
    10561 ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga attcgttgat
    10621 gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt tgaagacttt
    10681 gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg ttcttttaac
    10741 gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc aagccgcgcg
    10801 gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc agcgggatgc
    10861 ggcattgccg aaatgatcat ctcccagacc cagcgcgaag gattaagcga ggaagcggcg
    10921 cggcagaaag tctttatggt cgatcgcttt ggcttgctga ccgacaagat gccgaacctg
    10981 ctgcctttcc agaccaaact ggtgcagaag cgcgaaaacc tcagtgactg ggataccgac
    11041 agcgatgtgt tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat tctgattggc
    11101 gtctcaggac agaccgggct gtttacggaa gagatcattc gtgagatgca taaacactgt
    11161 ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc cacaccgcag
    11221 gacattatcg cctggaccga aggtaacgcg ctggtcgcca ctggcagccc gtttaatcca
    11281 gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt tattttcccg
    11341 ggcatcggcc tgggggttat tgcttccggc gcgtcacgta tcaccgatga gatgctgatg
    11401 tcggcaagtg aaacgcttgc tcagtattcg ccgctggtcc tgaacggcga aggtctggta
    11461 ctaccggaac tgaaagatat tcaaaaagtc tcccgcgcaa ttgcgtttgc ggttggcaaa
    11521 atggcgcagc agcaaggcgt ggcggtgaaa acgtctgccg aagctctgca acaggccatt
    11581 gatgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat ctaagcctgc
    11641 gcccggtagt gaaggctacc gggctatttc tctctccctt tttcagatct catccatact
    11701 gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca tccgaaaagg
    11761 aggaactatg aaggctgcag ttgttacgaa ggatcatcac gttgacgtta cggataaaac
    11821 actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg gtgtatgtca
    11881 taccgatctt catgttaaga atggcgattt tggtgacaaa cctggcgtaa ttctgggcca
    11941 tgaaggcatc ggtgtggtgg cagaagttgg cccaggtgtc acctcattaa aaccaggcga
    12001 tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact gtaatagtgg
    12061 taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg gcgggatggc
    12121 ggaagagtgc atcgtggtcg ccgattacgc ggtaaaagtg ccagatggtc tggactcggc
    12181 ggcggccagc agcattacct gtgcgggggt caccacctac aaagccgtta aactgtcaaa
    12241 aattcgtcca gggcagtgga ttgctatcta cggtcttggc ggtctgggta acctcgctct
    12301 gcaatacgcg aagaatgtct ttaacgcgaa agtgatcgcc attgatgtca atgatgagca
    12361 gttaaaactg gcaaccgaaa tgggtgcaga tttagcgatt aactcacgca ccgaagacgc
    12421 cgccaaaatt gtgcaggaga aaaccggtgg cgctcacgct gcggtggtaa cagcagttgc
    12481 taaagctgcg tttaactcgg cagttgatgc tgtccgtgca ggcggtcgtg ttgtggctgt
    12541 cggtctgccg ccggagtcta tgagcctgga tatcccacgc cttgtgctgg atggcattga
    12601 agtggtcggt tcgctggtcg gcacgcgcca ggatctaacc gaagccttcc agtttgccgc
    12661 cgaaggcaaa gtggtgccga aagtcgccct gcgtccgtta gcggacatca acaccatctt
    12721 taccgagatg gaagaaggaa aaatccgtgg ccgtatggtg attgatttcc gccgttaaga
    12781 ggcctttgct gcgagtgcca gtcctggtgt cgcagcatcg cactctctcc gacattaaat
    12841 ggctaaatgg attattgttc cttcctacgc aacaataatg atagtgttcc tgatgaagga
    12901 acactactga gagtaaagac actgatgaaa atggccaatc atccccgccc gggggacatt
    12961 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
    13021 attgctccct caacggcaag ccgattgctg accggaaaag cagctttgac accagaaatg
    13081 gcgattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
    13141 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgactacg ccgtctggtt
    13201 acgcaataaa aaaatggcgc ttcttaaagc gccattttca gcttaaagtt caaaagagat
    13261 tatatccctt cttcactctc ttttttcgct tctgccttct cgatttcacg ataccagcgc
    13321 ggatggtgtt tcttcgccca gcgacggctc accttccctt cgatcatccc tttaatcgac
    13381 cctttcaccc agaatgccat atacatatgg atcaggatgg cgtggatcag gatgataccc
    13441 gcagccgcgt ggatcagcag gctgtagcga acaacttgca tcgggaagta ctgcgcaaag
    13501 tacggacgcc agataatcac cccggtcacc agcagcacga aaatcatgct catgatcgac
    13561 cagaacatca tcttttgccc ggcgttgtac ttaccgacat ccgccacttt atgctcattg
    13621 cctttcaata cttcgacaat gttcaacagc cacggaatat ctttcttatc cgggatgttg
    13681 tgatgcacaa aacgcacaaa cataaacatc agtgcgacga aaatcgcaat gccgaaaaac
    13741 gggtgcaaaa tgcgt
//
LOCUS       DAEVZA010000131        13654 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEVZA010000131 DAEVZA010000000
VERSION     DAEVZA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.131"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..793)
                     /gene="rimK"
                     /locus_tag="LAF92_003973"
     CDS             complement(<1..793)
                     /gene="rimK"
                     /locus_tag="LAF92_003973"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBI9792900.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNAS"
     gene            complement(854..1577)
                     /gene="nfsA"
                     /locus_tag="LAF92_003974"
                     /pseudo
     CDS             complement(854..1577)
                     /gene="nfsA"
                     /locus_tag="LAF92_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            complement(1561..1848)
                     /locus_tag="LAF92_003975"
     CDS             complement(1561..1848)
                     /locus_tag="LAF92_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBI9792901.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            2008..2265
                     /gene="grxA"
                     /locus_tag="LAF92_003976"
     CDS             2008..2265
                     /gene="grxA"
                     /locus_tag="LAF92_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBI9792902.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(2295..2672)
                     /locus_tag="LAF92_003977"
     CDS             complement(2295..2672)
                     /locus_tag="LAF92_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBI9792903.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            2942..4627
                     /locus_tag="LAF92_003978"
     CDS             2942..4627
                     /locus_tag="LAF92_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBI9792904.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHFGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(4802..5338)
                     /gene="rcdA"
                     /locus_tag="LAF92_003979"
     CDS             complement(4802..5338)
                     /gene="rcdA"
                     /locus_tag="LAF92_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBI9792905.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            5422..6630
                     /locus_tag="LAF92_003980"
     CDS             5422..6630
                     /locus_tag="LAF92_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9792906.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            6630..7445
                     /gene="ybjI"
                     /locus_tag="LAF92_003981"
     CDS             6630..7445
                     /gene="ybjI"
                     /locus_tag="LAF92_003981"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBI9792907.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            7531..7815
                     /locus_tag="LAF92_003982"
     CDS             7531..7815
                     /locus_tag="LAF92_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792908.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(7856..9088)
                     /gene="mdfA"
                     /locus_tag="LAF92_003983"
     CDS             complement(7856..9088)
                     /gene="mdfA"
                     /locus_tag="LAF92_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBI9792909.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAVIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            9373..9969
                     /gene="ybjG"
                     /locus_tag="LAF92_003984"
     CDS             9373..9969
                     /gene="ybjG"
                     /locus_tag="LAF92_003984"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBI9792910.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            10027..10785
                     /gene="deoR"
                     /locus_tag="LAF92_003985"
     CDS             10027..10785
                     /gene="deoR"
                     /locus_tag="LAF92_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBI9792911.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(10832..12034)
                     /gene="dacC"
                     /locus_tag="LAF92_003986"
     CDS             complement(10832..12034)
                     /gene="dacC"
                     /locus_tag="LAF92_003986"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBI9792912.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            12281..12907
                     /gene="gstB"
                     /locus_tag="LAF92_003987"
     CDS             12281..12907
                     /gene="gstB"
                     /locus_tag="LAF92_003987"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBI9792913.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(12904..>13654)
                     /gene="yliI"
                     /locus_tag="LAF92_003988"
     CDS             complement(12904..>13654)
                     /gene="yliI"
                     /locus_tag="LAF92_003988"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HBI9792914.1"
                     /translation="SDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYLFIALGE
                     NNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQGMAMNPW
                     SNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAGTEQPVF
                     YWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRILTDRGQ
                     RIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         3297 a         3512 c         3380 g         3465 t
ORIGIN      
        1 gcgacgcatt cacctccatc accaacggcc cgcgattagc acgcagaata tccacaccag
       61 caacgtccag cgccatcgtt cgcgcggctt ttatcgcgat ttcacgctcc tgtggtgtga
      121 tactggcgac gcttgccgcg ccgccacgat gcaaattgga acgaaaatcg ccctctttcg
      181 cccgccgttc aatcgcagcg accacttcat cgccaacaac cagacagcgg atatcgcacc
      241 cttgcgcctc tttgatatat tcctgcacca gaatatgcgc gttcagaccg cggaaagcgt
      301 caatcacgct ttccgccgcc tgacgcgtct ccgccagcac gacgccaatt ccctgcgtgc
      361 cttcaaccaa cttgaccacc agcggcgcac caccgaccat gtcgattaaa tcgctggtat
      421 catccggcga atgcgcaatg cccgtgacag gcaggtcgat gccctgacgc gccagcagtt
      481 gcatggaacg caatttgtca cgcgcccggg caatggcgac cgactcattg agcggatagc
      541 tccccagcat ctcgaactgg cgcagtgccg ccgtcccata aaaggtaatg gcggtgccaa
      601 tacgcgggat cactgcgtca aaatggggta acttgcggcc tttgtagtga atagaagacg
      661 ccgcaggatt tatgttcatg tagcaagaaa gcggatcaag aatttcaacc aggtgaccgc
      721 gctgtatagc ggcttcacgc agccgcttac acgaatagag cgttccatcc cgggacaata
      781 tggcaatttt caccctgcac ctctctgtca gacctggtca aaagcccgcg tatcatacat
      841 cgacgtggcg gttttagcgc gtcgcccaac cctgtttgtg caaataatcc agaataaatg
      901 ggcggctttc tttaatgatt gttcggcgga tatgatcgct ccaggtatcc cggcgattat
      961 tgctgccacg ggtgaggtaa tattccgcca gttgctcgtc atactgcgcc agtgcgcctt
     1021 tatccagcgg ttgatagctg ttttcatgca ccaaaatgga ggccggtaaa cgcggcttaa
     1081 gatccggatt atccgcaggc cagccaaggc acagcccaaa cagcggcaga acatgctgcg
     1141 gtaatttaag cagtttcgtc accgcttcaa tattattgcg caggccgccg atatataccc
     1201 cgcccaatcc cagcgattcc gctgcgatta atgcattctg cgccatcatt gccgtatcaa
     1261 cgacaccgag caacagttgt tccgccaggc cgagctgagc atccggacag atctgtaaat
     1321 ggcggttaaa gtcggcacag aacacccaga actccgccgc ttgcgctacg tgttttttgc
     1381 ccgccggtca gcgtcaccag ctcttcacgt aacgctttgt cggtaatgcg aataatgcta
     1441 ctgcactgca aaaaactgga actggacgtc gcacgggcgc tgttaataat cgcctcacgc
     1501 tgcgcttcgg aaatgggttc atcagtgaaa tggcgaatgg agcgatggcc acaaataagt
     1561 tcaatggttg gcgtcattat ctttttctct ttctgaacgt gaatattgcg gtggacggtt
     1621 catcagctgt ggggcaagac gttttgccac ctgaagaata accaccaccg cagcgggaag
     1681 catgagcaaa acaccgagaa aaatcatcag aatctgcact tctggccgag aaaatggctc
     1741 aggcagcgac agggagtcgc ttaccgacag cagcgccacc gccagtagca tcattccgat
     1801 aaattccagt atcaacacgc ctttaggcaa tttaccgatc gcgcgcatac gcttccctct
     1861 gcaaagtgag ccttcagtct aaaacttttc actgtattgt gtttaacagt tatagctttt
     1921 agcaattaat gcaacaggtt aaacctactt tcagcgaata cattttagcg tgatcattac
     1981 aggcataaat ctatgaggag agaaataatg caaaccgtta tttttggtcg ttcgggttgc
     2041 ccttactgtg tgcgtgcaaa agatctggct gagaaattga gcaatgaacg cgatgatttt
     2101 cagtatcagt atgtagatat tcgtgcggaa gggatcacta aagaagatct acaacaaaag
     2161 gcaggtaaac ccgtagaaac cgtgccgcag atttttgtcg atcagcaaca tatcggcggc
     2221 tataccgatt ttgctgcatg ggtgaaagaa aatctggacg cctgatcgtc tgacaagccc
     2281 tcgcgttgag ggctttactg attttttctg tgctgtggtt taaacaaact actgataaat
     2341 aagaaacaca gtgcccccag cgcacaccag aacaccgcgc ttagtaacca tgccagctct
     2401 tgccagaatg agcgcgtcgg tgaaaaaaac agccgcataa tgagcattga acagggtgcc
     2461 gccagcattg cgccaaatag aggtttcagg acttctctac gctgtgaaaa gaaactggca
     2521 actgctccag gaagaatgaa aaatagcaag ccgatttcag gatgcccggc agcccgaaaa
     2581 gcgcctttca tgtgcgtcgc cagaaaaagg cacaccacaa tgaagaggac aaaacagcag
     2641 attgcccccg cccaacgttg tttatgtttc actcgttcct cctgacactg cgtctatcga
     2701 acacattttt cgccagtgtg gcgttcagta agataaagcc gcttcgcatt ccatgctaat
     2761 ataggccaac gcaattcata tagccgttga tacctaatgt gattacacta gtaaaatata
     2821 ttgttacttt actatcgttt aggtgcgctg aatgaatctg cgccctgaat tctggtaaaa
     2881 aacattatcg taaattacca tttctttcaa cagcttacta gtaaacaaga agttagcctc
     2941 cgtgaatata aacgtcgccg aattgttaaa tgggaattac attctgttat tatttgtggt
     3001 cctcgcgctt gggctatgtc tcggaaagtt acgacttggt tcgatccaac tgggtaattc
     3061 cattggcgtt ttagtcgtat cgctgttatt aggccaacaa catttcagca ttaacaccga
     3121 tgcgcttaat cttggcttta tgctgtttat tttctgcgtc ggggtcgaag ccggaccgaa
     3181 ctttttttcc attttttttc gcgatgggaa aaattaccta atgttagcac tggtgatggt
     3241 tggcagtgcg ctggtgatcg ccttagggtt aggtaagctg tttggctggg atattggcct
     3301 gacggccggt atgttagcag gctctatgac gtcgacaccg gttctggtcg gtgctggcga
     3361 tacactgcgt catttcggca tggaaagcag gcagctctca ctggcactgg ataatctgag
     3421 cctcgggtat gccttaacct atttaatcgg tctggtgagt ttgattgttg gtgcgcgtta
     3481 cttgccgaaa ttgcagcatc aggacttaca gaccagcgcc cagcaaatcg cccgcgaacg
     3541 tggcctggac actgatgcca accgtaaggt ttatttaccg gtgatccgcg cctatcgcgt
     3601 cggcccggag ctggtggcct ggaccgacgg caaaaatctg cgtgaactgg gtatttatcg
     3661 acaaaccggc tgctacattg aacgtattcg acgtaacggg attctggcaa atccagacgg
     3721 tgatgccgtg ctacaaatgg gcgatgaaat agcgttggta ggctatcccg acgcccatgc
     3781 ccgactcgat cccagcttcc gtaacggtaa agaagttttc gatcgtgacc ttctcgacat
     3841 gcgtatcgtc actgaagaag tggtcgttaa aaaccataac gctgtaggta aacgtctcgc
     3901 acaactgaag ttgaccgatc acggttgctt ccttaaccgc gtcattcgta gccagattga
     3961 gatgccgata gatgacaacg tcgtgcttaa caaaggtgac gttttacaag tcagcggtga
     4021 tgcccgtcgc gtaaaaacca tcgccgatcg catcggcttt atctcgattc acagccaggt
     4081 cactgacctg ctggcatttt gcgccttctt tgttattggg ctgatgatcg ggatgatcac
     4141 cttccagttc agcacattca gtttcggcat ggggaacgct gccgggttgt tattcgccgg
     4201 aattatgctg ggctttatgc gtgctaacca cccgaccttc ggttacattc cgcaaggtgc
     4261 attaagcatg gtgaaagagt tcggcttgat ggtgtttatg gcaggcgttg gtctgagcgc
     4321 cggtagcggt attaataacg gcctgggcgc gattggcggt cagatgttga ttgccggatt
     4381 aattgtcagt cttgtgcccg tggttatctg tttcttgttc ggtgcttatg tattgcgaat
     4441 gaaccgcgca ctgttgttcg gcgcaatgat gggcgcacgc acctgcgcgc cggcaatgga
     4501 gatcatcagt gatacagctc gcagtaacat ccctgcgctg ggctatgcgg gcacctacgc
     4561 aatcgccaac gtcctgctga cgctggcagg gacaatcatc gtcatggtat ggccaggatt
     4621 aggataaaac tgaagttgcc ctgaaaatga aatttttttg cacaaccgca gaacttttcc
     4681 gcagggcatc agtcttaatt agtgccactg cttttctttg atgtccccat tttgtggagc
     4741 ccatcaaccc cgccatttcg gttcaaggtt gatgggtttt ttgttatcta aaatttatct
     4801 actaccctgc aaccctctca accatcctca aaatctcctc gcgcgatagc ggcttacggt
     4861 cggtgacaaa atgcagcgtc atcccctcaa taaacgcatc aagcgcgcgg gcggttccgg
     4921 gttcaaacca ttgttcgagc gtttgctgac tgcgctgcat ccagttttgc attaccgttt
     4981 ttaatagcgg ttttcggcta gccagcgcgt agagctggta catcagctcc atattatccg
     5041 gcgttgcaac ctgtgagctg tagatcatat cggtgatagc ctggcatgcg ccttgagcat
     5101 cactaacatc gctaaaaaat gcctgatatt gccgggacat aatctcagta aaactgctga
     5161 acgcctccag caacaactca tcaattcctg aaaaatagta ggtcatcgat cccaacggta
     5221 ccccggcaag ggtagcaatt ttgcggtgcg taacagcatg tattccgtaa agtttcaccg
     5281 cctccagcgt ggcctggata attttttctc gccgttgcgg atcgttagcg cgacgcatgt
     5341 catttcctct ctctgcaatt gtgtacaaat gtacacaacc ttgctaatgt tgtcttccct
     5401 cttcttattt tgacgtgatc tatgaccgta aattcttcac gtaatgcatt gaaacgccga
     5461 acctgggcgc tgtttatgtt cttctttttg ccaggcctgt taatggcgtc ctgggcaacc
     5521 cgtacgcctg ctatccgcga tattctctct gtctcgatcg ctgaaatggg cggtgttctc
     5581 tttggtctgt cgatcggttc gatgagcggt attctctgct cggcgtggtt agtgaaacgc
     5641 tttggcacgc gtaatgtcat cctggtcacg atgtcctgcg cattgatcgg gatgatgata
     5701 ttaagtctgg cactctggct gacatcgccc ctgctctttg ccgttggtct cggcgtcttt
     5761 ggggcaagtt ttggttctgc ggaagtggcg ataaacgttg aaggtgccgc cgttgagcga
     5821 gaaatgaata aaacggtttt gccaatgatg cacggttttt atagcctggg cacgctggca
     5881 ggcgctggtg tcgggatggc actgacggcc tttggcgttc cggcaacggt gcacatttta
     5941 ttggcggcgc tggtaggtat cgcacctatt tatatcgcca ttcaggcaat ccctgacggt
     6001 acgggcaaaa atgctgccga tggcacccag catggcgaaa aaggcattcc tttttatcgc
     6061 gatatccagt tgctgctgat aggtgttgtg gtgctggcga tggcctttgc cgaaggttct
     6121 gccaacgact ggttaccctt attaatggtt gacggtcacg gttttagccc tacttccggc
     6181 tcgctgattt atgccggttt taccctgggg atgaccgttg gacgcttcac cggcggttgg
     6241 ttcatcgacc gttacagtcg cgttgccgtg gttcgggcca gtgcactaat gggggcgttg
     6301 ggtattgggc tgattatttt tgtcgatagc gcctgggtcg ctggggtgtc tgttgtactc
     6361 tggggactgg gtgcctcgct gggcttcccg ctgaccattt ctgccgccag cgataccggc
     6421 cccgatgcac caacccgcgt cagtgtggta gcaacgaccg gttatctggc tttcctcgtc
     6481 gggccgccgc tgctgggcta tctcggcgaa cattatggat tacgtagtgc aatgctggtt
     6541 gtactggcgc tggttattct cgcggctatt gtcgcgaaag ccgtcgccaa acccgatacc
     6601 aaaacgcaga cggcgatgga gaatagttga tgagcattaa attaattgcg gtagacatgg
     6661 atggtacttt cttaagcgat caaaaaacct ataaccgtga gcggtttatg gctcagtatc
     6721 agcaaatgaa agcacaagga attcgctttg tagtcgccag cgggaatcaa tattatcagt
     6781 tgatctcttt cttccctgaa attgctaatg aaattgcctt tgtggctgaa aacggcggct
     6841 gggtagtgag cgaaggcaaa gatgttttta atggcgagct gtcgaaggat gcgtttacta
     6901 ctgtcgtgga acatttgctg acgcgcccgg aagtggaaat tattgcctgc ggaaaaaata
     6961 gcgcctatac gctcaaaaaa tatgacgatg ccatgaaaac ggtggcggaa atgtattatc
     7021 accgtctgga atacgtcgat aactttgaca acttagagga tatcttcttt aagtttggcc
     7081 tgaatctttc cgatgaactg attccacaag tacaaaaagc attacatgag gccatcggcg
     7141 atattatggt gccggtccac accggcaacg gcagcatcga tctgattatc cccggcgtac
     7201 ataaagccaa tggccttcgc caactgcaga aattatgggg aatagacgac agcgaagtgg
     7261 tggtctttgg cgatggcggt aacgatattg agatgctgcg tcaggcaggc tttagttttg
     7321 caatggaaaa tgccggcaat gcggtcgtcg cagcggcaaa ataccgggca ggctccaata
     7381 accgtgaagg cgtactggat gtgatcgata aagtgcttaa acacgaagcg ccatttgacc
     7441 aataaaaacg gcagcacgcc cggcaacaca atacctgaat gaaggctgca taattcgcgg
     7501 aaattaaccc gctgcattat ttcaggcgaa atgtttatga aaaattgtct actgttgggc
     7561 gcgcttttaa tgggctttac tggtgtggcg atggcgcaaa gtgtcaccgt cgatgtgccg
     7621 agtggataca aagtggttgt ggttcctgat tcagtcagcg ttccacaagc ggtcagcgtc
     7681 gcgactgtgc cgcaaaccgt ctatgtggct cctgcgccag ctccggctta ccgcccgcat
     7741 ccttatgttc gccatcttgc cagcgtaggt gaagggatgg ttatcgaaca ccagattgac
     7801 gaccatcacc attaattcga gaatgcctga tcgcacaaag caatcaggca tttttttacc
     7861 cttcgtgaga atttcccatc tgtttatctt ttaaaaagat aaccatcagc gacagccaca
     7921 aaattccgtt gacaaggttg aagagattaa actgtccgtt gcccccgttc agccaggcat
     7981 gtttgctgat ttcaatacca acggtaaaga tcagcatttg cagcattccc atcgccgcag
     8041 aaaccgtacc tttactcata tcgctggcaa acagggttaa tcgcaccagt cccgcattcg
     8101 ccagaccaat accgaaagca taaatactta acccggcagt catccataaa tacgcgtgcg
     8161 atgagataac cgttgccgca gcagcgacca acagaccaat cataatcggc cagccgccca
     8221 taattatcag cgaacgtacg gtgcggcgcg aggtcaaacg cgctaacagc aagttacccg
     8281 caattaacgc cccgaaaata ggcacttgca gcaagccata ttcatagctg ctcaactgct
     8341 cgccggtaat gatgataatc ggcgactggg cgatccacgc cagcaatggc aggctaacga
     8401 atcccagcgc cagcgccccc gccacaaagc ggccgttctt cagcaccagc ttatagtcac
     8461 gaccgagttc tttcagtgac agtttctcgc ctatacgcgt ggcggtttca ggcatggctc
     8521 gttgcagacc gaaaaaggag atcgctgcca atgcggcaaa caaaacaaac atcccctccc
     8581 agggcagcac atggatccac gccgcgccca ccagcggacc aagtagcgga gcaatcagcg
     8641 ccacgttcgc catcagcgcg gtgatcttga tacaaaccgc ctcttcgaag gattcctgaa
     8701 ttgcggcgta tcccacagcg ccaatgaaac agaggcttat gccctgcaag aagcgcaaca
     8761 gagtgaattg ttcaatgttt tgcgccagta atattgccag acaggtgatg ataaaccaca
     8821 ccacccccgc cagcatcacc ggacggcgac caatacgatc cgacagcggc cccaggagcc
     8881 attgtaaaaa catcccgccc gccagatatg cggtcattga agtaggaacc caatcaatga
     8941 ccgcctgata ttgttccacc acggccaaca taccgggttg aatcatatcg ttgccgatat
     9001 aggttgaaaa ttcgtaaagc accagacaga gagggaaaag taacgcctga cgtccaagcc
     9061 tggcaccgga agctaattta ttttgcatgc aatttcttcg ccaataataa tcgcgcagag
     9121 tttaataaaa gcacagctaa cgagaaagcg aattttgtag ctgaaaccac ggttaagcac
     9181 attcttacat tattgcgagt atagctacgc tttctttaag ttttatttaa cctatgcccg
     9241 ttacaattac ccaccgtaaa caggccgctt gagggaaata agacgatgcc gctttaccca
     9301 gtttaacctg cactttattc tcaacgactt gcctgtattg gctccctttt aatcactttg
     9361 cgtcgggaag ttatgctgga aaatttgaat ctctctctat tctctcttat taacgcgacg
     9421 ccagactcgg ctccgtggat gatctcgttg gcgattttta ttgctaaaga tttgattacc
     9481 gtggtaccgc tgctggccgc ggtgctttgg ttgtgggggc ttacagcaca acggcaactg
     9541 gtgataaaaa tcgctatcgc gctggcggtc agcctgtttg tgtcctggac gatgggacat
     9601 ctttttccgc acgaccgacc ctttgtcgaa aatatcggct ataacttcct gcatcatgcg
     9661 gcggatgact cattcccaag cgaccacggt acggtgattt tcacctttgc actggcattt
     9721 ttatgctggc atcgcctgtg gtccggctca cttttaatgg tgctggccgt cgtcattgcc
     9781 tggtcgcgcg tttatcttgg cgtgcactgg ccgctggata tgctcggtgg attgctggca
     9841 ggtatgattg gctgccttag cgctcagatt atctggcaag cgatggggca taaactctat
     9901 caacgtctgc aatcgtggta tcgcttctgt tttgcattac cgatccgcaa aggctgggtg
     9961 cgtgactgat ctctgctaaa aagtgtagta ttgagcggct cgcttcaata actattcaga
    10021 gggattatgg aaacacgtcg cgaagagcgt atcgggcagc tgctgcaaga attaaaacgc
    10081 agcgataagt tacatcttaa agacgccgcc gccctgcttg gggtttcgga gatgacgatt
    10141 cgtcgcgatc tgaacaacca cagtgcgccc gtcgttttgc tcggcggcta tattgttctg
    10201 gaaccgcgca gtgccagcca ttacctgtta agcgatcaaa aatcccgcct ggtggaagaa
    10261 aaacgccggg cggcaaaaat ggctgcgaca ctggtagaac ccgatcagac tctctttttt
    10321 gactgtggca ccaccacgcc gtggattatt gaagcgattg ataatgaaat cccttttacc
    10381 gccgtttgtt attcgctaaa tacctttctg gcgctgaaag agaaacccca ttgccgcgca
    10441 tttctgtgcg gtggtgaatt tcacgccagc aacgccattt tcaaacccat cgattttcag
    10501 caaacgttga ataatttttg cccggatatc gctttttatt ccgcggcggg cgtgcatgtc
    10561 agtaaaggcg ctacctgttt taatcttgaa gagttgccgg taaaacactg ggccatgtcg
    10621 atggcgcaaa agcatgtgct ggttgtcgac cacagtaaat ttggcaaggt gcgtccggcg
    10681 cgcatgggtg acctgaaacg ctttgatatt gtggtgagtg attgttgccc ggaagatgag
    10741 tatgtgaagt acgcgcagac gcaccgtatt aagttgatgt attaatgacg tataaccgga
    10801 tgacgtttcg cgccatccgg ttatcagaag attaagagaa ccagctgccg aaccactgat
    10861 ggaatttcat catcacgaaa tcccacatcc gaccaaagaa tccgccctct tccacatttt
    10921 ccatcacgat cagcggacgc tgctcaatgg atttaccgtt aagctggaaa tcaatggtcc
    10981 cgacaacctg accttttttc agcggtgcgg taagctgcgg ttccgttaac gtgtagctcg
    11041 ccttcaggtt tttcagctgc ccacgcggta tggtcactga gcccgcttca cctgccccaa
    11101 gattcacttc gctcttatca ccaaaccaga cgcgctgagt cacaaaggtg gcatcaggtt
    11161 taattggcgt cacggtttca aagaagcgga aaccccaggt caataatttc tcagactcat
    11221 taaaacggat acggtcagtt ttcgccccca gcactacgga gattaaacgc atatcgccct
    11281 gggtagccga agcaaccaga ttatatcccg cgcctgccgt ggttcctgtc ttcatgccat
    11341 caacattcag attgctgctc cacagcagac ggttacggtt aggctgacga attttgttga
    11401 aggtgaactc tttctcttta tgaatggcgt actcttccgg cacatcgtgg atcaatgctt
    11461 tacccagcaa tgccatatcg cgcgcggtac tgaactgccc cggcgcatcc agaccgtgca
    11521 ccgtctggaa ggtagtgttg gtcagaccca gttttttggc ataaccattc atcaaaccaa
    11581 taaatgactc ctggctcccg gcaacgtaat cggccagcgc aatacaggcg tcattaccgg
    11641 actggataat cacacctttg ttcaagtctg ccaccgaaac ctgatcgccc ggtttgagga
    11701 acattaccga tgaaccacgc agtgccggat ttcccgtcgc ccaggcatct ttaccgaccg
    11761 tcaccatatc ggtgagttta atcttatcgg ccttaagcgc ctgcccaacc acatagctgg
    11821 tcatgatttt agtcaggctc gcgggatcca gtttctcatc cgcgttgcct tctgccagca
    11881 ctttaccgct ggcgtaatcc attaaaatcc atgcacgcgc atccacgctc ggcgcttcaa
    11941 cggtttgttc cgccgcgaat gccgttgggg caaaaaggaa taaaaatgca gaacccgctg
    12001 caagaccacg aaggagagag gagtattgcg tcataaatgc cacccgagta tccattcaaa
    12061 aaaaatacgt tatatcgccg tatgataagt tcggtgatta ataacgcaca cacaaactta
    12121 aagaaaccgt aagttggtaa agtttttaaa gtttatgcaa tatcctgccg cttcgctgct
    12181 tctggtgtca tttttttgcc agctgttgct taaaagttaa ctttacttca ccatcggagt
    12241 cctgttcagc ctgtggctgc ctgtcggaaa aggggtgagt atgattacgc tgtggggtcg
    12301 gaataattca accaacgtaa aaaaagtatt gctgacgctc gaagaactgg aactacctta
    12361 tgagcaaatt ctcgcgggcc gtgagtttgg gataaatcac gatgctgatt ttctggcgat
    12421 gaaccctaac gggctggtgc cgttgttgcg tgacgacgaa agtgatctca ttctttggga
    12481 atcaaacgcc attgtccgct atctggcggc acagtacggg caaaaacgtc tgtggatcga
    12541 ctcaccggca cgtcgtgcgg aagcggaaaa atggatggac tgggcaaacc agacgctcag
    12601 taatgctcat cgcgggatcc tgatgggatt agtcagaaca ccaccggaag agcgcgatca
    12661 ggccgccatt gatgccagtt gcaaagagtg cgacgccctg tttgccctgc tcgatgcgga
    12721 actggcaaaa gtaaaatggt tctctggcga cgagtttggt gtgggcgata tcgctatcgc
    12781 accgttcatc tacaatttgt ttaacgttgg cctgacctgg acaccgcgtc caaatctgca
    12841 acgctggtat cagcaactca ctgaacgccc cgcagtgcgc aaagtggtga tgattcccgt
    12901 tagctaattg cgtgggctaa ctttaagtaa ttccccactg gactcgtcgg tgagaacgta
    12961 taaataaccg tcgggtccag tgcgaacatc acgaattcgc tgccctctgt ccgttaaaat
    13021 acggccatct tctgtcactt tgtcgccgtt gacgctcatc acaatgacat ctttatcttt
    13081 cagcgcgcca ataaataatt tttgctgcca ctgggggaat ttatcgctgt tatagaaggc
    13141 catgccgctc acagcgggcg aatctttcca gtaaaaaaca ggttgctcgg tcccggcgac
    13201 gatctcccct ttcgcttccg gtatcttaaa gcctgaatag ttgattcccc aggttgccag
    13261 cggccagccg tagtttttgc ctttttgcgg gatattaatt tcatcaccac cgcgcgggcc
    13321 atgttcattc agccacagtg cattactcca cggattcatc gccattcctt gcggattacg
    13381 aatgccataa gaccagatct cggcgcgcac accggattcc tttataaaag gattatcatc
    13441 cgggatttcg ccctggtcgg tcagacgcac cagtttgccc tgtaatttat ccagatcctg
    13501 cgccgtcggg cgctgattgt tttcgcccag agcaataaaa agataacctt taccgtcgaa
    13561 taccagccgc ccgccaaaat ggttgccggt agacagtttt ggcatctggc ggaagacggt
    13621 gcggaagtcg gtcacttttg agagatcatc actt
//
LOCUS       DAEVZA010000132        13605 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEVZA010000132 DAEVZA010000000
VERSION     DAEVZA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.132"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            167..1354
                     /locus_tag="LAF92_003989"
     CDS             167..1354
                     /locus_tag="LAF92_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792915.1"
                     /translation="MFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTREL
                     TIETATADSAGNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEA
                     RSKYLRAVVNEELISTAKVTNKKSLAFTGGTNGDQSNISTAAYLRAVKVLNNAPYMHT
                     AVLGLGCYDNAAISAVEDTGLLGTDYVSCAVYHFPFSCKDKWTQSRVVFGLSGAAYAA
                     KARGVKKNSDVGGWHYSPAGEERAVIARASLQPLYPEDTPDEEAMVKGRLNKVSVGTS
                     GQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQMKHNPDGITESGLTKGMT
                     KLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEVVWACCPTGVARRIQGVPL
                     LIK"
     gene            1364..2179
                     /locus_tag="LAF92_003990"
     CDS             1364..2179
                     /locus_tag="LAF92_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792916.1"
                     /translation="MSKNFFQSGVFLGNGLSRFALNSDLVQLMESARASAEPTTDPVI
                     NNNPEPAAQTNDNAPSATAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVANFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKVRRKAQTGAAKLARKKSMKLRRKRLG"
     gene            2215..2796
                     /locus_tag="LAF92_003991"
     CDS             2215..2796
                     /locus_tag="LAF92_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792917.1"
                     /translation="MAPIAYGVYSQADGVSPYLKVTLTNSQYQVTGYISQGAAMNMAQ
                     NWEAPFTGMSMGSVSGALGGFVQVGTETTSVARWNSLMVWEGGTPPTFTLPVTFIALN
                     NPFIEVSGAIAALTAMISPELKAANVGGRIPERVTLNIGRRINITDVAIQDLSFDLDA
                     PRDSNGYFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            2808..3317
                     /locus_tag="LAF92_003992"
     CDS             2808..3317
                     /locus_tag="LAF92_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HBI9792918.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTFAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGAIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKASGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            3477..4588
                     /locus_tag="LAF92_003993"
                     /pseudo
     CDS             3477..4588
                     /locus_tag="LAF92_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
     gene            complement(4782..4937)
                     /gene="hokD"
                     /locus_tag="LAF92_003994"
     CDS             complement(4782..4937)
                     /gene="hokD"
                     /locus_tag="LAF92_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI9792919.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVTVF
                     TAYESER"
     gene            complement(5272..5541)
                     /locus_tag="LAF92_003995"
     CDS             complement(5272..5541)
                     /locus_tag="LAF92_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepL"
                     /protein_id="HBI9792920.1"
                     /translation="MTSWIAEAKAKADNLRLSQKRAQKHEFKQKVEAAARKYAYLKNK
                     RSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNDQWGIPAGFRG"
     gene            complement(5538..6116)
                     /locus_tag="LAF92_003996"
                     /pseudo
     CDS             complement(5538..6116)
                     /locus_tag="LAF92_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            complement(6146..6946)
                     /locus_tag="LAF92_003997"
     CDS             complement(6146..6946)
                     /locus_tag="LAF92_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBI9792921.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPQSLPEALRLAAELAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAATR"
     gene            complement(7110..8144)
                     /locus_tag="LAF92_003998"
     CDS             complement(7110..8144)
                     /locus_tag="LAF92_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBI9792922.1"
                     /translation="MKKPLVTRNEIAEAIALHTTCMPTREIPGAIANYFMITRRFYTR
                     TDKAVINKLLIAEIRDYLIEQGRLRYATVAAEMRKEAHRVTGKNLNIEKPAPVTSATP
                     TPAVNVIPNIGDTIDSQTLLKMVNEARKLCSEKPVRNNDFIARVKDELEGETYEIFVG
                     QKNGAEIDIITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPTPSIPQTLPEALR
                     LAAELAEQKMQLEQQLVAAAPKVDFADRVSVANGILIGNFAKVVGLKQNALFSWLRQN
                     GILMAFGARKNVPRQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRKLLD
                     AGLLKPVAIG"
     gene            complement(8141..8362)
                     /locus_tag="LAF92_003999"
     CDS             complement(8141..8362)
                     /locus_tag="LAF92_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBI9792923.1"
                     /translation="MVNANPYARQEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSADVQGDL"
     gene            8983..9495
                     /locus_tag="LAF92_004000"
     CDS             8983..9495
                     /locus_tag="LAF92_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792924.1"
                     /translation="MAKISSLCVMVVSAIFLSGCQSNGAEYAADVYDTAQLNSKQETR
                     VVNIISVLPAKVKVDNTANKQAAQTFGAVLGAVAGSVAGYNIKGTSTLGAVAGGAGGG
                     ALGAAAGSMVSDKSIVEGVSLTYKEGTKVYTSTQVGRACQFAPGIAALISTKENETRI
                     QPNATCPDKK"
     gene            9507..10046
                     /locus_tag="LAF92_004001"
     CDS             9507..10046
                     /locus_tag="LAF92_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBI9792925.1"
                     /translation="MKKIICYLCLAALNTGNVFANSLQSQIAAIAQAESEGRAREQQL
                     EDARLEQIRKQENAERLRRERAAAAANAREKARIAAENERRAKREAELTAEKKRNQAY
                     EDELRKLELEKMKLELQAKAARVKRENDFIEQELKERAARTDVIQSEADANRNISSGS
                     KDLLQSEGKAREKKASSWW"
     gene            10362..11621
                     /locus_tag="LAF92_004002"
     CDS             10362..11621
                     /locus_tag="LAF92_004002"
                     /inference="COORDINATES: protein motif:HMM:NF037405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBI9792926.1"
                     /translation="MRKKLTFFGTIVLLLLCYVIYPCVWPVAGMMLYVAYITIVGFDK
                     KNKSSSKPPIESSNRPSIKPLNKSLLNSLNKPLFKPLLKPLNEPLVKPLTKPLTKPSD
                     ERLYGHRRGLLAWWQTVDADAENWLDIFCKGCESPAEEAFLRKMVAEFNLKPKSGMLV
                     SPDLVLEMQVPYKRYRFDFVVNGRQIIEIDGAAWHSSPEQIERDRIRDEYSIKEGFMV
                     LRIPAKTVFNTPNVAIEQVKKTLEKTPNYTMPVRQSVINQQPLKKFSFTKLLSGINNN
                     LNCQMIKQEALFPFLLAIETEKRQLEMIVSEIEMEMRVASMLPQDRMLYENAKSRLEA
                     LLQNDDSLNYTTKPTYQWDKITKPSPVENQQIQESIDSEYKDIMNERELRIAKLLHRC
                     KVDPIFSMKFRDKLKMYNYPTITDIFK"
     gene            11752..12318
                     /locus_tag="LAF92_004003"
     CDS             11752..12318
                     /locus_tag="LAF92_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792927.1"
                     /translation="MTPRQLLEDVKTRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRFTDQTCKSLACPADFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGEVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            12329..12940
                     /locus_tag="LAF92_004004"
     CDS             12329..12940
                     /locus_tag="LAF92_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9792928.1"
                     /translation="MGLNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKSRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            12955..13470
                     /locus_tag="LAF92_004005"
                     /pseudo
     CDS             12955..13470
                     /locus_tag="LAF92_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
BASE COUNT         3610 a         2956 c         3342 g         3697 t
ORIGIN      
        1 tgttcttgcg gttactgagt ccaattacga agatgttctc ggcgaaccgc tgaagccgtc
       61 ctccggctca cagttcgaac caattcgcca tgtgtacgaa gctattcagc aaacgtctgg
      121 ttatgttgtc cgtgctgttc cggatgatgc gaagttcccg attattatgt tcgatgaatc
      181 aggcgaaccg gcttacagtg cgttgccata cggttctgaa attgaacttg atagcggcga
      241 agcctttgct atctacgttg atgatggtga tccgtgtatt tctcctaccc gtgagttaac
      301 catcgaaacg gcaacagcgg acagcgcggg taatgaacgc ttcctcttaa aactgaccca
      361 gacgacttcg ctcggtgtgg taacgaccct ggagacacac actgtgtctt tggcggaaga
      421 agcgaaagat gacatgggcc gcttgtgtta tctgcctacg gctctggaag cccgttctaa
      481 atatctgcgc gcggttgtta atgaagagct gatttcgacg gcgaaagtaa caaataaaaa
      541 atcgctggcg ttcactggcg gtaccaacgg cgatcagtcg aacatttcca ctgctgctta
      601 cctgcgtgcg gtgaaagtgc tgaacaatgc gccgtacatg cacaccgctg ttctcggcct
      661 gggttgctat gacaatgcgg cgatctctgc tgttgaagat accggtttac ttggtaccga
      721 ttatgtaagc tgtgctgtct atcatttccc gttctcctgc aaagacaaat ggacccaatc
      781 ccgcgtggtc ttcggtctgt ctggcgcggc gtatgcggcg aaagctcgtg gcgtcaagaa
      841 aaactccgat gtcggcggtt ggcattactc accggctggt gaagaacgtg ccgtcattgc
      901 tcgtgcatca cttcagccgc tgtatcctga agacaccccg gacgaagaag ctatggtcaa
      961 gggccgactc aataaagtat ctgtaggtac ttcgggccag atgatcatcg atgatgcttt
     1021 aacttgctgc acgcaggaca actatctgca tttccagcac gtcccatccc tgatgaacgc
     1081 aatcagccgt ttctttgtcc agttagcccg acagatgaag cataacccgg acggcattac
     1141 tgagtctggc ctgactaaag ggatgaccaa acttttggat cgctttgtcg cctccggcgc
     1201 tctggtggct cctcgtgatc ctgatgctga cggtacagaa ccgtatgtgc tgaaagttac
     1261 gcaggcggaa ttcgataaat gggaagtagt ctgggcctgc tgcccgactg gcgtagcccg
     1321 tcgtatccag ggcgtaccgc tgcttattaa gtaaggaaat acaatgagca aaaacttttt
     1381 tcaatccggg gtatttttgg ggaatggact gtctcgtttc gctttgaact ctgatcttgt
     1441 gcagttgatg gagtctgccc gagcaagcgc cgaaccgaca acagatccgg ttattaataa
     1501 taatccggaa ccggcggcac agactaacga taacgctcca tctgccacgg ctcctgagca
     1561 aatcctggaa gggaaagacg gtaaagaatg gaccgtcgaa caggcgcacc agatgattct
     1621 ggaagctgca aatcgaagtg ctatgcagaa tgcgttgagt gatgcggccg acgccgtttt
     1681 cgcctgggct gatagcggtg atctgacttt cgactccctt gatggtttcg ttcaggctat
     1741 cgctggtatc tctgatgacg acgactccga agttacagaa gaacaggacg atgcctataa
     1801 cgaagcatgg gcaaatgttg ctaacttcct cgcagcatgc ggtgtagatg atgacctgat
     1861 cgaagcactg gctgacgatg aagacgacga cgcagctgct gatgttggtg cctctatcgc
     1921 tggtttagat agcgacgacc gcgacgaact ggaagcggcg tttgttgttg ctggcacttc
     1981 tgatgaaatg ctgactgaag catttaagaa ggttgttcgt aacggtgaga tcaaactcat
     2041 ccgtaaacgc ctgcgtaaaa aacgtctgac tgcggctcaa aaatcggcgc tgaaaaaagt
     2101 gcgtcgaaaa gcccagaccg gcgcggcaaa acttgcccgc aaaaagtcaa tgaaactgcg
     2161 ccgtaagcgc cttggctaaa ggaggaggcc ggagaactcc ggcctttaac ttgaatggct
     2221 cctatagctt atggggttta cagccaggct gacggtgtat cgccatatct gaaagttact
     2281 ttaacgaact ctcagtacca ggttaccgga tatatcagcc agggagcggc aatgaacatg
     2341 gcccagaatt gggaagcgcc gtttaccggt atgtccatgg gatctgtttc tggtgctctg
     2401 ggtggttttg tgcaagtagg tactgaaaca acgtcggttg cccgttggaa tagcttaatg
     2461 gtttgggaag gggggactcc gccgacgttc acgctgcctg taactttcat tgctttgaac
     2521 aatccattca ttgaagtttc aggcgctatc gccgcgttga cagccatgat tagcccggaa
     2581 ctaaaagcgg ccaatgttgg tggtcgaatc ccggagcgcg tgacgctaaa cattggtcgc
     2641 cggatcaaca tcaccgatgt cgccatccag gacttaagtt ttgatctcga tgcgccaagg
     2701 gacagtaatg gatatttcct gaaaaacacc gtcaacctcc agttgaccgg ttcttcgata
     2761 tataacagct ccgatattgt tcgggcgttc cagtaaaagg attttatatg gggcacaata
     2821 acactaaggg aaaccgtaaa tttattaagg gccgctatac tgccaacgcg gccaaaggcg
     2881 aacgactggt atcttctgaa ttccagctca cttttgcagg ccatgaagat atcagcgtac
     2941 tggttcgcac gtcgcaaatc cctgaaatga ctcgagagga tgtggaggac tatggtccga
     3001 atggtgtgaa gttcaaccag cacggagcaa ttcgaaactc tggggaaatc caggtccagt
     3061 gcgtggagac tatcgaaggc gatattcttc agttcattaa ggatcgcatt gcggcgaagg
     3121 actatgttga tatcacgatg gctgctaccc ctgaatccaa agcttccggg gttaacgctg
     3181 tgacaaaagc tgctacaaca attgaaatgt tggactgcaa aatctacagt gatgcaatcg
     3241 actttagtac cgaagatgtg actgccgctg tgcgcccgtc acttcgtatc gtctacaact
     3301 ggattgagtg ggattaagag tcatcccttg tattttaaag ctccttcggg agctttttta
     3361 tttggagagg aaagggtgca ttgaggatac ctgacacacg aagagtggcg gggatctctc
     3421 ccataagcgc taacttaagg gttgtggtat tacgcctgat atgatttaac gtgccgatga
     3481 attactctca cgataactgg tcagcaattc tggcccatat tggtaagccc gaagaactgg
     3541 atacttcggc acgtaatgcc ggggctctaa cccgccgccg cgaaattcgt gatgctgcaa
     3601 ctctgctacg tctggggctg gcttacggcc cggggggatg tcattacgtg aagtcactgc
     3661 atgggctcag ctccatgacg ttgcaacatt atctgacgtg gctctcctga agcggctgcg
     3721 gaatgccgcc gactggtttg gcatacttgc cgcacaaaca cttgctgtac gcgccgcagt
     3781 tacgggttgt acaagcggaa agagattgcg tcttgtcgat ggaacagcaa tcagtgcgcc
     3841 cgggggcggc agcgctgaat ggcgactaca tatgggatat gatcctcata cctgtcagtt
     3901 cactgatttt gagctaaccg acagcagaga cgctgaacgg ctggaccgat ttgcgcaaac
     3961 ggcagacgag atacgcattg ctgaccgggg attcggttcg cgtcccgaat gtatccgctc
     4021 acttgctttt ggagaagctg attatatcgt ccgggttcac tggcgaggat tgcgctggtt
     4081 aactgcagaa ggaatgcgct ttgacatgat gggttttctg cgcgggctgg attgcggtaa
     4141 gaacggtgaa accactgtaa tgataggcaa ttcaggtaat aaaaaagccg gagctccctt
     4201 tccggcacgt ctcattgccg tatcacttcc tcccgaaaaa gcattaatca gtaaaacccg
     4261 actgctcagc gagaatcgtc gaaaaggacg agtagttcag gcggaaacgc tggaagcagc
     4321 gggccatgtg ctattgctaa catcattacc ggaagatgaa tattcagcag agcaagtggc
     4381 tgattgttac cgtctgcgat ggcaaattga actggctttt aagcggctca aaagtttgct
     4441 gcacctggat gctttgcgtg caaaggaacc tgaactcgcg aaagcgtgga tatttgctaa
     4501 tctactcgcc gcatttttaa ttgacgacat aatccagcca tcgctggatt tcccccccag
     4561 aagtgccgga tccgaaaaga agaactaact cgttgtggag aataacaaaa atggtcatct
     4621 ggagcttaca ggtggccatt cgtgggacag tatccctgac agcctacaaa acgcaattga
     4681 agaacgcgag gcatcgtctt aacgaggcac cgaggcgtcg cattcttcag atggttcaac
     4741 ccttaagtta gcgcttatgg gatctctccc cgccaggtct cttacctttc agattcgtag
     4801 gctgtgaaga cagtgacctc cgtctggccg gttcggattc gtacctcgca gaggtctttc
     4861 ctcgttacca gtgccgtcac aatgacggtt aaacagatga cgatcagagc gattaacatc
     4921 gctttttgct gcttcatagc ctgcttctcc ttgacctttt ggtcggtaag aggctaatct
     4981 acgtatgcta agcatagata ttgcctcaga ttaatgttaa gcgtcttgca ggacgcgtaa
     5041 tgttatctgg ggctttcttc tatctgcttt tcgggtaatg cctgaagcag atagcctcaa
     5101 gcacccgcag cgattgtatc aatgtctggc tttttttcta tagaaatcac ctggaagggt
     5161 gaatacccac atcagaagaa acgttgcagc aaacatgatc cctaatggcc agaccgcgcc
     5221 aaagaaaatc catactgaaa acgccaggtg actccagatt agagcaatct atcaccctct
     5281 gaatcccgcc ggtatacccc attgatcgtt atctttattt ttggctaaaa ccgcattaag
     5341 agcttcgttt accgtcatgc aatgcggtag gttatcgaag tttgatatcc cgccaatatc
     5401 aggcgaacgc ttgttcttca ggtaagcata tttccgcgca gccgcctcta ctttctgctt
     5461 gaactcatgt ttttgagcgc gtttttggga taaccgcaga ttgtcagcct ttgcttttgc
     5521 ctcagcgatc catgaagtca attttttgag tctggacgtt ccggcaccgc cggaaactga
     5581 tctttttgtt tttttaactt gtgacttctt attctttatt gccacgtcat cctgacaggg
     5641 ggagggggta tcattttgac atggggtgtg gatataaaat taaataaagc caatgtctta
     5701 gcgataacag ctttaacctt ggttgccgct gaagagatct ttaatttgct ttcagtcagc
     5761 gcatttttgg cttgttgtgc gaaggccaaa aaggatggtg taaaccggta caggttagcg
     5821 cgacgttcac ggtgatcgcc gataacaatc tctacagaca ggattccttt gtttacagct
     5881 tcacggaatg cacgaacgac ggttgattgg ctataaccag tttctgccgc gatcaggcgg
     5941 tgaggcttgt gaatgaagta ttcactggtt gttgccgcga gatttgcaca ttgcgacagg
     6001 atatgcccgg cgctacggga tagaccggag tgtgttacaa agcaggccaa ttcatagcca
     6061 gaaaaagtaa aatcgctcat cgttatacag ctcaggaaag tgactttagc cagcattaca
     6121 atgctggtgg ttcttactac gtctgttagc gcgttgccgc gacaggtacc agcacaccag
     6181 catcaagcaa tcgcttcatc agccactgct gacctttgcc ggttatacga gtcgtgaaag
     6241 aaatcctgct tccattgctt gtatcgatca cggtttcttt aagggtgaaa tatccacggg
     6301 atatgtattc ttgtttgggg acgttcctgc gttcaccggt tgcgatcaga attccgttat
     6361 cacgcaacca ggtgaagaga tagttttggc ccaggccgag cactttggca tagttgccga
     6421 ttagaacccc gctggcggta gcaacgcgtt cggcgaattc gactttaggt gcatccataa
     6481 gcattttttg ctccagccgt tgcttttgct ctgccagttc ggcagccaaa cggagagctt
     6541 cagggagact ctgcggaata gcaggttgta atcttccagc tcgatagtcg ataaatgtct
     6601 ggtttacctt cagccgaaac gcgggagaaa tccaaccagc gtactccact gcgagcaatt
     6661 catgggcaaa agtgccgccg ccacggcctt cgaacgaaac tatgcaattc tgcatagttt
     6721 ctttttcaag ctcttcgatg agctgtttag ctgacagcgt tcttagccat tgagctggcg
     6781 ctttatgggc accgagtccg ctcgctctgt gtagagcatt aaggttgtaa cggccagcgc
     6841 ggtcggtcgt aatttcaaca ccacaaataa cgggaagagt ggttgaagga tcgacatttt
     6901 gatgaaggtt tgatatattc atatccgcat tgaatgtttg ttgcattttt tctccaaatt
     6961 tgcatcaacc ttcaatcacc agctcgaaat ggtgattctt tgcacttaga aaacgaaatt
     7021 tattagagca aatttttcta actcgatcca gatcgggttg gtcgatctgc tcagaaacct
     7081 gtcagtttgc tggcaggttt tttcttttgt taacctattg ctactggttt taacaaacca
     7141 gcatcaagta gcttgcgagt taaccactgc tggcctttac ccgttaattg gggcgtcagc
     7201 cgtatctggt agccattttc atcatccaac accacttctt tcaccgtgaa atacccggcg
     7261 ttgatgtact gttggcgcgg tacgtttttg cgcgcaccaa aagccatgag aatgccgttc
     7321 tggcgcaacc atgagaaaag ggcgttttgc ttaagtccaa cgacctttgc aaagttcccg
     7381 atcaggattc cattggccac tgatacccgg tcggcaaaat cgactttagg ggctgcggcc
     7441 accagctgtt gttccaactg cattttctgt tctgctaact cggcagccag gcgtagggct
     7501 tctggtaatg tctgggggat cgatggggta ggggagtttg cctgctgtaa ttcttccagt
     7561 ttgtcgatca gcgaacggcg gaccgctttt gactcgcgcg cggcaactcg cagggcttgt
     7621 ttgtaggtca ttgttataat atctatttct gcgccgtttt tttgacctac gaaaatttcg
     7681 taggtttctc cttcaagttc atccttaact ctggcaatga aatcattatt gcgaactggt
     7741 ttttcgctac ataatttccg tgcctcattg accatcttca acaatgtttg gctgtcgatt
     7801 gtgtctccga tgttggggat gacattcacg gctggtgttg gcgtagctga cgtaacaggt
     7861 gctggttttt caatattcaa atttttaccg gtcactctgt gtgcctcctt tctcatttct
     7921 gctgccactg ttgcgtaacg tagacgtcct tgttcaatca aataatccct gatctcggct
     7981 atcagtagct tgttgatcac agccttatct gttcgggtat aaaaacgtct ggttatcatg
     8041 aaatagttgg caattgcgcc ggggatctcc cgtgtcggca tacaggttgt atgcagggcg
     8101 atcgcttcgg ctatttcatt acgggtgacg agaggttttt tcataaatcc ccctgaacgt
     8161 cggcagagaa ggggaggttc cagtaactaa gtgaattgcg cgagttagtt gaaaaacggg
     8221 cagtaaaaat gcaggggcca tcaggcaatt gagagcgtgc ttcgtcttct gttgctgcga
     8281 tgacgaagtg atagtggtgt tttttacagg aatagaaacg ccagatgaat tcttggcgtg
     8341 cgtaaggatt ggcattaacc atagttacgg cctcacaatc aggtttaaca acctgctacc
     8401 cgctgctaaa caggtggcag gacgtgacgg ggttagcaga ctggcgattg tgaaaccagc
     8461 aggccgaagc ctccccatca cgtcccacca taatttgggc gtaacgcggt tttacggaca
     8521 caaaaatacc gcaatatcgg atatctgcgg ctgtccgcac aatcattcag gctgctaaac
     8581 ccggtcgcag aatttgctac gacggcggaa ctataagcct gaacgattaa aaggtcaata
     8641 tgatgcgaaa agatagcatt cgtgacttaa aaagacaaat ttattagagc aacatgctgt
     8701 tggtataggt ttccatatat gcgcatataa aattggaaat tgtatataat tagagagtga
     8761 gttagtgtca atattaaacc aactcaacta aatcaagtgt aaattattag cagagcatat
     8821 aatgtcgata gttgattttt ttagacttaa tataacgcag cttcagcaat gaagatcgta
     8881 ttttttataa taattgatat acatcaatag gattaatctt gtttgtattt ggcttatgtg
     8941 atagattttc ttggattata ttttttttct gggatgattc taatggctaa aatttcttct
     9001 ttatgcgtca tggttgtttc tgcaattttt ctctcagggt gtcagtctaa tggtgctgaa
     9061 tatgcggcag atgtgtacga cacagcccaa cttaattcaa aacaagaaac aagagtagta
     9121 aatataatct ctgttctccc agccaaagta aaagttgata atacagctaa taaacaagca
     9181 gctcagactt tcggtgctgt tctcggtgca gtagctggta gtgttgctgg atataatatt
     9241 aaaggaactt ctaccttggg tgcagttgct ggtggtgctg gcggtggagc tcttggcgca
     9301 gctgctggtt ctatggtaag tgataaatca attgttgaag gtgtatctct tacttacaag
     9361 gaaggtacta aagtctatac atctacacag gttggtagag cttgccaatt tgcccctgga
     9421 atcgcggctt tgatttcaac gaaagaaaac gaaacgcgca ttcaacctaa tgctacatgt
     9481 cctgataaaa aataactcaa taagatatga agaaaattat ttgttattta tgtttggctg
     9541 ctttgaatac aggcaatgta tttgctaatt cattgcagag tcaaatagct gctattgccc
     9601 aggcagaaag tgaaggtcga gcaagagaac agcagctgga ggatgccaga ctagagcaaa
     9661 ttcgcaagca agaaaatgct gaacgtttaa gaagagagag ggctgcggca gctgcaaatg
     9721 cccgcgagaa agctcgtata gcggcggaaa atgagcgcag agcgaagcgt gaagctgaat
     9781 tgaccgcaga gaaaaagcga aatcaggctt atgaagatga gcttcgtaaa ttagaacttg
     9841 agaaaatgaa gcttgagcta caagcgaaag ctgctcgagt taagagagaa aacgatttca
     9901 ttgagcagga gctaaaggaa agggctgcaa gaactgatgt gattcagtct gaagctgatg
     9961 caaatcgtaa catctcttca gggagtaagg atttattgca aagcgaaggt aaagcaaggg
    10021 agaaaaaagc cagtagctgg tggtaatttc cgttgaacca tcctctctta ttacactcta
    10081 tgtagcaaat ggcaacccgt agaatcgatt gttaatatca attctacaag gttgccaatg
    10141 atttttttaa attttggtgt aatagtcatc ttgtagagct gtatattctc tgtaaaaata
    10201 tcatttttaa attagcataa tcaatcaatt gtgatggcga tattaagtgt taatgtatat
    10261 tagtttagag tgtgatttcc gttggtgttg gtcatctttt atttcgggca tgtgatttga
    10321 ttatataagc cagtcccctt tattgatgca aagagtttgg tatgcggaag aagttaacat
    10381 tttttgggac tattgttcta ttactgttgt gttacgtgat ttatccatgt gtatggcctg
    10441 ttgcaggcat gatgctatat gtagcatata taactatcgt aggttttgat aaaaaaaaca
    10501 aatcatcatc taaaccaccg attgagtcgt caaatagacc gtcaatcaaa ccattaaata
    10561 aatcgttatt aaattcgtta aataagccgt tatttaagcc gttattaaag ccgttaaatg
    10621 aacccttagt taaaccgtta acaaagccat taacaaaacc gtcagatgaa cgattatatg
    10681 gacaccgacg tggactgtta gcatggtggc aaactgttga cgctgacgca gaaaactggc
    10741 ttgatatatt ttgcaaaggt tgcgaatctc cggctgagga ggcatttctt cgtaaaatgg
    10801 ttgcagagtt taatcttaaa cctaaatctg gaatgttagt ttctcctgac ttagtgcttg
    10861 aaatgcaagt gccctataag cgttatcgtt ttgattttgt cgtaaatgga cgccaaatta
    10921 tagaaattga tggtgcagca tggcattcat ccccagaaca aatagagcga gatcgtattc
    10981 gtgatgaata ttcaataaag gaggggttta tggtgctacg tattccagca aagactgttt
    11041 tcaacactcc taatgttgct atagagcaag tgaagaaaac acttgaaaaa acaccgaact
    11101 acacaatgcc agttagacag agtgttataa atcagcagcc tttaaagaag ttctcattta
    11161 caaaattact tagtggcata aacaataatt tgaattgtca aatgattaag caagaagccc
    11221 tatttccatt tcttttagct attgaaacgg aaaaacgtca acttgaaatg atagtatcgg
    11281 aaattgaaat ggaaatgcgt gttgcaagta tgttgcccca agatagaatg ctatatgaaa
    11341 atgctaagtc tcgtcttgag gcattactcc aaaatgatga tagtttaaat tacactacga
    11401 agccaactta tcaatgggat aaaattacta aaccgtctcc tgttgagaat cagcaaattc
    11461 aggaaagtat tgatagcgaa tacaaagata ttatgaatga gagggagcta aggattgcaa
    11521 agctcttaca cagatgtaag gtagacccta tattttctat gaaatttaga gataagctta
    11581 agatgtataa ttacccaact ataactgata tattcaaata attattaagg ttgctataca
    11641 tcctgtagca gcgcgattcg tatgaattat gatatccata ataacagttg gcacaatgga
    11701 caaatgacat ctcgtagaat cggttaacac accagattct acgaggtttc aatgacacca
    11761 cgacaattac tcgaagacgt caaaacccgc ttcacacctt tgattgcgga tgaacctgcc
    11821 ttactggagt ctctgctaag aaaagcattg ggaacctacc aggatagggc ggggcacatc
    11881 aagcggatac gcttcactga tcagacctgt aaatcacttg cttgcccagc tgattttctt
    11941 gcgctcgtat cggttacaga tcacaccggc gatcttgtct actccgatgt ttacgatggg
    12001 aatatcgagc ttgaagatac tcatcgagcg gtatacccgc tgaatgtgtc atatctggct
    12061 aatttacgtg atatggatct ggataatggg gaagtgccac ctgaaatcat tgggttactt
    12121 tctgactatc tggaagtgtt aatcgcgata cctaacactg atcgcctgcg aagaatatct
    12181 atcgcgggga aactcgatgc cagcaattta tccgacgaga acacgctgta tcagcgaaag
    12241 ctggatctgg aagagaaaat gagcgcaaca agggcaatta tcccggggat tgttcttttc
    12301 tcatccatgt tgaagtgagg gggctgatat ggggcttaat gttgcttcag taaagtctta
    12361 tgtatcttcg gcattaacga cgacattatt tggctccggc gttggtgagc gggaagttgg
    12421 taagctgacg tcaatcatca tgaacaaaat gctgttcgcg caaggatggc agttctctgt
    12481 cgaagttgat ggactggagg gggcagactt ctttgccaaa gacattacct accacgatta
    12541 cagcatcgaa tatgaaacga ttaaaatcgg cggagggaat atccttcaac caacagagcg
    12601 ttcgcctggg cagataacaa tgatggtcag ggataccgtt gatggcctcg ttttggactg
    12661 gtttaagacg gcaaaaagtc gggtgatcaa tccggacggt accgggaata taccgtctaa
    12721 atatttgctc aatgtgcgta tttatcggtt gctgtcttcc ggcttaacca aactggaaaa
    12781 tgagatgacg gtattcccgg tcactaccgg cgatgtcacc tatgcgcggg atcaggttac
    12841 ggaatttaag tcattcccaa tgaccttcgc attgcacagc acgtttaacc aatcctcaag
    12901 ttctttggct tcccttctgg gctttagttt ttctctttga attaaggagc aaggatgctt
    12961 ttaccccttt tcccgctacc atcgcggcca actgaattga tccagttccg tcagccaaat
    13021 attgctgatg cgatgcgttt caactcgata acaccggagg aacaagaaca acagacaacg
    13081 gcgtatttaa aagccttgct ggctgaaccc gcgaaacatg atcccctgac atggacggcg
    13141 caggaccgga ttaccgcgtt atggtggata tttaccggct cccgtgaaac accggtcgag
    13201 acattcacct acacctgtaa acattgcggt aaagagcatt attacgattg cgatatgaat
    13261 gctctggctg aagatatcca ggtcctggaa gtggaaccgt tcattgacga tattgaggtg
    13321 cctgtagagg gagtacctta tcaatggcgt atcgtgccgc ttgatggttg ggcaatggaa
    13381 atgctggaga tgcgccgtgc agcattgcca cctgaagacg acgcggaatt caaagaagcg
    13441 atcgttgatt tgcgtttttg ggaattcgct taaccggttg gaatgataca gaagcgcaac
    13501 gtcgagcgtt tgggcttaaa tcaggacaag atccatcatt ccagcaaaaa gccgcttata
    13561 cgttagccaa tgttcttgat atggggggac tggtatctgg gtaat
//
LOCUS       DAEVZA010000133        13536 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEVZA010000133 DAEVZA010000000
VERSION     DAEVZA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.133"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1408
                     /gene="trkG"
                     /locus_tag="LAF92_004006"
     CDS             <1..1408
                     /gene="trkG"
                     /locus_tag="LAF92_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Trk system potassium transporter TrkG"
                     /protein_id="HBI9792929.1"
                     /translation="WLYSLSMLPPMVVALFYKEKSLFVFFITFVIFFCIGGGAWYTTK
                     KSGIQLRTRDGFIIIVMFWILFSVISAFPLWFDSELNLTFIDALFEGVSGITTTGATV
                     IDDVSSLPRAYLYYRSQLNFIGGLGVIVLAVAVLPLLGIGGAKLYQSEMPGPFKDDKL
                     TPRLADTSRTLWITYSLLGIACIVCYRLAGMPLFDAICHGISTVSLGGFSTHSESIGY
                     FNNYLVELVAGSFSLLSAFNFTLWYIVISRKTIKPLIRDIELRFFLLIALGVIIVTSF
                     QVWHIGMYDLHGSFIHSFFLASSMLTDNGLATQDYANWPTHTIVFLLLSSFFGGCIGS
                     TCGGIKSLRFLILFKQSKHEINQLSHPRALLSVNVGGKIVTDRVMRSVWSFFFLYTLF
                     TVFFILVLNGMGYDFLTSFATVAACINNMGLGFGATASSFGVLNDIAKYLMCIAMILG
                     RLEIYPVIILFSGFFWRS"
     gene            1546..2340
                     /locus_tag="LAF92_004007"
     CDS             1546..2340
                     /locus_tag="LAF92_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBI9792930.1"
                     /translation="MSEKLKIVYRPLQELSPYAHNARTHSTEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFIPTEIDELLTDVLPGTGNEEEPYTTKIDTPVYEPS
                     GGKPDISELYDDTKTQELISRIRSASLDPDIEKFLLCAAERHTVFNFSRIADYYAHAP
                     AEIQCLFEESALVIIDYQQAIENGFVRMTQRMVEIMHGGEEEEEYA"
     gene            2333..3265
                     /locus_tag="LAF92_004008"
     CDS             2333..3265
                     /locus_tag="LAF92_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792931.1"
                     /translation="MRDDFCAFILTHGRPDKVLTYRTLRRAGYTGKIFIVVDDEDKTR
                     HQYMAEFGEQVLVFSKADIASRFDEADNFGDRRSIFYARNACFDLAKLVGCKYFIQLD
                     DDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLAYYESIPALSIAMSQGGDFLGDNGGH
                     ASWVKRKAMNSFICSVDRPFSFMGRINEDVNTYTNLGRCGELFMTIGAVQLGQKQTQK
                     NSGGMTELYLDSGTYVKSFYSVMYSPSCVKISLMGASHKRIHHQVTWNNAAVKILHEK
                     YRKKTPCISMGVTNDSVFESRVSGSVPDDCTTNR"
     gene            3201..3452
                     /locus_tag="LAF92_004009"
     CDS             3201..3452
                     /locus_tag="LAF92_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792932.1"
                     /translation="MIPYSKVESLAACRMTAQQIADVLDVDLNRLKENREAMTDFYAA
                     IRKGRAKGEAELRAALFKLARKGDAFALRELLRVDKNQD"
     gene            3456..4550
                     /locus_tag="LAF92_004010"
     CDS             3456..4550
                     /locus_tag="LAF92_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBI9792933.1"
                     /translation="MSRPDWGALQQEYIAEYTRSGISPVAWCEARGLNYATARRYIKK
                     TPKNAQKEMRKTAQKSARKKTAQTAQRQNGKSQKKKAVSDALLIEGDTEEISFCPDEF
                     GISDQQAKFAMLVAQGKKPTEAYRLAGYEGQGATANSNASRMLRNARVYRAISYFRNQ
                     YQKRYTADLDLLVSQLMAIVQADPNQLAQFRRVNCRYCWGENHLYQWRDIAEFDKAAA
                     QASRDGKPEPEYGGLGFVDNAIPNPDCPKCCGEGTGQLYMADTTLLDGDARQLYAGSK
                     LGKYGVEILLEDKAAARRELLRLLSAGGALCADKRLQELEIERRRIENQKLRKEIETV
                     EDNEHPQPVAININVVDARLRSDEDDLSDA"
     gene            4531..5832
                     /locus_tag="LAF92_004011"
     CDS             4531..5832
                     /locus_tag="LAF92_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HBI9792934.1"
                     /translation="MISPTLNVPQARFLSMPHKFKAYIAGFGSGKTWVGCGGICKGIW
                     EHPGINQGYFAPTYPQIRDIFYPTVEEVAADWGLNVKINEGNKEVHFYYGRQYRGTTI
                     CRSMEKPQTIVGFKIGNALVDELDILPKEKARTAWRKIIARMRYKIDGLRNGIDVTTT
                     PEGFKFVYEQFVKAVREKTELASLYGLVQASTFDNEKNLPADYIPSLLESYPPELIKA
                     YLLGRFTNLTSGTVYHQFDRKLNNCEEVEQPGEPIYIGMDFNVGKMAGIVHVLRLGLP
                     CAVTEIINAYDTPDMIRLIKERFWLYDGHDYRKVREIYIYPDASGDSRKSSNASTTDI
                     AQLKQAGFNVVVNSSNPPVKDRVNSMNAMFCNANGERRYKVNVKRCPVYAESLEQQVW
                     DDKGEPDKKSGNDHPNDAGGYFIVKQFPIVKPTGRVTSLRI"
     gene            5835..7241
                     /locus_tag="LAF92_004012"
     CDS             5835..7241
                     /locus_tag="LAF92_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HBI9792935.1"
                     /translation="MADISTPNLDYNDMLEAWDINDALMGGTLEMRRQGENYLPKWPN
                     EDEDAYKKRLSVATLLPVYEESIKQNIGRIFAEPTVLSEETPEKIREYAENIDMEGSR
                     LDVWAQQFFSLAFQYGVAHALVDYPRTDMNEIRTKADENAAGGRPYVTMLNPRQVIGW
                     KSKVKKGKVVLTDLRIKEVIIIDGDDFGQKKVEQIRHIMPRRVEIYRRSEGTNGESVW
                     MLHESWNTSRDDIPLVTLYTKKTGFMRGTPPLLNLGLLNIKHWQSQSEQDNILHVARV
                     PLLVAYGLDRNEELTVGASTATIFEDRTKNGLEYVEHSGAAIESGETSLEKLENQMRH
                     AGAKLLRAENTSTKSVDQTNEERMQEHSPLYTMANSLEDALDNILQIMAEWSGESCGG
                     NVDVRTELDVSAQVFDSSSALAVQSLRQGGDIRQIDAVRVLQALKFIDQDSRPEDVID
                     ELKNQSVMLMEINDANRE"
     gene            7225..8337
                     /locus_tag="LAF92_004013"
     CDS             7225..8337
                     /locus_tag="LAF92_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792936.1"
                     /translation="MQTVNERLRDESIAHAVWISRYSTGVAARMVKILNDSDAELTAR
                     LLVALDSLDPGSFTVTRLESLLASVREVNRAAINSMFTSLSGELNELAIYEAGYQLSL
                     FDSLLPDFVADVHPLVGISSDALYAAAMARPFQGRLLSEWALDLEADRLRRITNTVRQ
                     GFLLGDTNEQIARKIRGHVSKGFQDGALQMSRANAASIAKTAVGHLAATARESFASAN
                     NDLIKGKQWLSTLDNRTTPQCRIRDRLKYTLDNKPVGHSVPYLHGPGKIHFCCRSTET
                     FILKSAKELGIDVRDISPAERASMDGVVAGDTTYREWFLRQPYTRQKQIVGETRAKLI
                     RDGGMSPDEFYTDKGEWLTLKQLRERDAQVFRKAGI"
     gene            8442..9206
                     /locus_tag="LAF92_004014"
     CDS             8442..9206
                     /locus_tag="LAF92_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792937.1"
                     /translation="MKLKTVEINGKQYAEIDTAGLPVYVHDDGKEIGFDAPLAIKKIT
                     ELNGEAKNHRLAKEAAEEKLAKFAAIEDPKKAIEALEMLSKIDQKKLIDAGQVDQVKA
                     EITKNFQQQLDEEKQRSQMLETQLYDSMIGGSFAGSKYIADKIAIPADLLQARFGQAF
                     KVEEGKIVAYDASGNKIYSRAKPGELAQFDEALEFLVENYPQKDYILKASGNNGGGSR
                     PTQHDVGQKTMKRSAFDALDVAGKQNALKDGITIVD"
     gene            9305..10444
                     /locus_tag="LAF92_004015"
     CDS             9305..10444
                     /locus_tag="LAF92_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 coat - protein 5 family protein"
                     /protein_id="HBI9792938.1"
                     /translation="MAGNTLTGLIPTIYTALDVVSREQVGFIPAVAKNAKADAAAKDQ
                     TVTAPVAPEAKTEDIVPGPSAPNTGDQNIGTVDVKITKSKMAPVKWNGEEQLALGPSG
                     TYNTILADQFKQAFRALANEVEADLGALYFGASRAVGTAGTTPFGVKDDLSDAALARQ
                     VLEDNGAPTTDLQMVLGSTAIANLRGKQSVLFKVNESGTEQLLREGVLGRLEGFNIHS
                     SAGVKRAPKVAATGYLVNGEKKEGDVLISIDTGSGSISAGQIVTFAGDPNQYVVAAAT
                     SNLITLAAPGLRQDLADDTAITVVGAFTANMAFDRNAFLLASRTPAMPEGGDNADDVM
                     NVTDPISGITFQIALYRQYRQVRYEVGLAWGVSSVKPAHGCLILG"
     gene            10487..10663
                     /locus_tag="LAF92_004016"
     CDS             10487..10663
                     /locus_tag="LAF92_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792939.1"
                     /translation="MAGLTKEQRAQREAAQKTQIELVVMVTDYQMFPGAPTIANVHPD
                     EVDNWKAMGWKTQE"
     gene            10667..11062
                     /locus_tag="LAF92_004017"
     CDS             10667..11062
                     /locus_tag="LAF92_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein singed"
                     /protein_id="HBI9792940.1"
                     /translation="MITYVTCDDVDNAFGNAWTSENAKNKAVLMANAWLNGFNLKINP
                     SRIPEEVKLAGAYAARIASFGKLFQQKNDSGVVISKAVSADGVSVSKSFSELPANSTA
                     LLEPDLQLAIALLKPYGLSRSQVRVVRGG"
     gene            11062..11445
                     /locus_tag="LAF92_004018"
     CDS             11062..11445
                     /locus_tag="LAF92_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBI9792941.1"
                     /translation="MGLREEIQSEVAAAFDEDLADAVSDFSGSYVTHRHWDPVTETGG
                     ESTAIYTGRGVLTRYKLGRIDGINILHGDLKLTALVCEVTDKPAVDHIIEIYDPVSRQ
                     LQRYEVITASVDPSASVYSIQLRRA"
     gene            11446..11826
                     /locus_tag="LAF92_004019"
     CDS             11446..11826
                     /locus_tag="LAF92_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBI9792942.1"
                     /translation="MAKAWDIEPSIFAGMIEEDVGLKIRYIAIQILTAIDIAAPVDTG
                     RFRNNNLVSLQHPDFGISDNVDPNGTIAVQRGIGVISKAANYGVIYIQNNLPYAEALE
                     NGHSQQAPTGVYANAFHGVLQAYK"
     gene            11823..12215
                     /locus_tag="LAF92_004020"
     CDS             11823..12215
                     /locus_tag="LAF92_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HBI9792943.1"
                     /translation="MKFTEIRNTVISRMTAQTIIDGKDVLYPNGPTFDPSGKLIWARL
                     SNIPGQAGVNEIGAGPIVYRTGIIIIQLFVPAGSGSKLITETADKLRELFEFQDDDRL
                     SYQAVSSIAVGEKNGWFQLNLQIPYRAL"
     gene            12242..13204
                     /locus_tag="LAF92_004021"
     CDS             12242..13204
                     /locus_tag="LAF92_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792944.1"
                     /translation="MSSGAKVISAFIRETVAGTTPASGDWSLLKRTSWGVKPTQNKGE
                     NNEIGGSRMAQGATPGTVDVGGDVGTKFRWGQHDDFLASCFGAEWSGDSLTMGNERIT
                     FSLATYASDVGIASVVRGAQVGSWKMQIPNDGDITATVTFAGLDWESKADDTNFIKGE
                     PVDSAGKLRYSFKEVSAVSLNGVAGGNGFCIDSFDIQFDNKLQTQRCIGTGSPYAGAN
                     IPTTFTPSGTVTLSWSKAAWEIWSKTLTGETVPFSFTLSNGEGAYTFSFPKVQVSGEW
                     PDGGNSDIIQVQLSITAADEAPTITRKKNSPAAVIAKASAEAIS"
     gene            13355..>13536
                     /locus_tag="LAF92_004022"
     CDS             13355..>13536
                     /locus_tag="LAF92_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9792945.1"
                     /translation="MVASSDNDQYRSRNALIRRHIEKMDASLHVGTKEFDISKVSEVD
                     SVDDLLIDNAARYLLK"
BASE COUNT         3602 a         2769 c         3582 g         3583 t
ORIGIN      
        1 ttggctctat agtctttcaa tgttgccacc aatggttgta gcattgtttt ataaagaaaa
       61 aagcctgttc gttttcttta taactttcgt tatatttttt tgcattggtg gcggagcgtg
      121 gtatacaact aagaaatctg gcattcaatt acgtacccgt gatgggttta ttataattgt
      181 aatgttttgg attttgtttt ctgttattag tgcattccct ttatggtttg actcagaact
      241 taatttaacg ttcattgatg ctctgtttga aggggtttct ggaataacaa caacaggagc
      301 aactgtaatt gatgatgtta gttcattacc tcgggcatat ttgtactatc ggtcacagtt
      361 aaattttata ggtggtttag gagttattgt tctagcggtt gcagtattgc cattattggg
      421 tattggtggt gcaaagcttt atcagtcaga aatgccgggg ccatttaagg atgacaaact
      481 cactccccgc ctggccgata cgtcacggac actgtggata acttattctt tattaggtat
      541 tgcttgtatt gtctgttata gacttgcagg aatgcctttg tttgatgcta tttgtcacgg
      601 gatttccaca gtttcgcttg gtggtttctc aactcatagc gagagtatcg gatattttaa
      661 taactatttg gttgagctgg tggctggttc tttttccctg ctatcggctt tcaacttcac
      721 tctttggtat attgttatta gcagaaaaac gataaaacct ttaatcagag atattgaact
      781 tcgtttcttt ctgttaatag ccttaggggt gatcattgtt acctctttcc aggtctggca
      841 tataggtatg tatgacttgc atggaagttt tattcattcg ttttttcttg ccagctccat
      901 gctcactgat aatggtttag ctacgcagga ttatgcaaac tggcccacgc acacgatagt
      961 gtttttgctg ttgtcaagtt tctttggggg atgtataggt tcaacctgtg gtggaattaa
     1021 gtcacttcga tttcttatac ttttcaaaca aagcaaacac gagataaatc agctttctca
     1081 tcccagagcg ttgttgagtg taaatgtagg agggaagata gttacagatc gtgtaatgag
     1141 gtctgtatgg agtttctttt ttctttatac tctcttcacg gtgtttttta tactggtgtt
     1201 aaatggtatg ggatatgatt ttcttacatc atttgcaaca gtggctgcat gtattaataa
     1261 tatgggatta ggttttgggg ctactgcatc gtcattcgga gtgcttaatg acattgcaaa
     1321 atatttaatg tgcatagcta tgattcttgg tcgccttgaa atttatcctg ttattatatt
     1381 gttttcaggt tttttttggc gctcctaata tatggctgat ttataattgt gagtttaata
     1441 ttatgttgac tcactcattg atccaatacc taactttacc agcaacacct ccgcccccag
     1501 tagcactggc tgctggggtg cgttttattc ataaagcaag gctgtatgag cgagaaatta
     1561 aagatagtct atcgcccatt acaagaattg tcaccgtatg cgcacaacgc caggacgcac
     1621 agtactgagc aggtggcaca actggtagaa agtattaagc aattcggctg gactaatccg
     1681 gtgctgattg acgaaaaggg cgaaattatt gcgggtcacg gtcgtgttat ggcggctgaa
     1741 atgctcaaaa tggattctgt tccggtcatt gttctgtctg gcctgacgga tgagcagaaa
     1801 aaggcgtacc gcctggcaga taatcgcctg ccgatgaatg ctggctggga tgaagatctg
     1861 ttgcggatgg agctgtcgga cctaatcaat gctgattttg atgtctccct gacaggcttc
     1921 atcccgacag aaattgatga actgttgacg gatgttttgc ccggtacagg aaatgaggag
     1981 gagccgtata cgacgaaaat tgatacgcct gtttatgagc cgtcgggcgg taaaccggat
     2041 atcagtgaac tgtacgacga tacgaaaact caggagctga tcagccggat acgttcggcg
     2101 tcccttgatc ctgatattga gaaattcctc ctgtgtgcgg cagaacgtca cacagtgttt
     2161 aatttcagca gaattgcgga ctattacgct catgcccccg ctgaaattca gtgccttttc
     2221 gaggagtcgg cgctggtgat cattgattat cagcaggcta ttgaaaatgg atttgtccga
     2281 atgacgcagc gcatggtgga gatcatgcat ggcggggagg aggaggagga atatgcgtga
     2341 tgatttttgc gcctttattc tgactcacgg gcgaccggac aaagttctga cttaccggac
     2401 gttgcgtcgt gctggctata ccgggaaaat ttttatcgtt gttgatgatg aagataagac
     2461 acggcatcag tacatggctg aatttggtga acaggtgctg gtattttcca aagccgatat
     2521 tgccagtcgt tttgacgaag ccgataattt tggtgatcgc cgctcaattt tttacgcccg
     2581 taatgcctgt ttcgacctgg caaaactggt cgggtgtaaa tacttcattc agctcgatga
     2641 tgattatcac gagttccagt ttcgggtgga tcgcaactat gaccaggcct atttcccgat
     2701 aaggaaactg gatgcgatcc tttctgaaat gctggcgtat tacgaatcaa tacccgcgct
     2761 ttccatcgct atgtcgcagg gcggggattt tcttggtgac aatggcggcc atgcttcgtg
     2821 ggtgaaacgc aaggcaatga acagctttat ctgttcggtt gatcgaccgt tctcattcat
     2881 ggggcgcatt aacgaggatg tgaatacgta cacgaatctc ggtcgctgtg gtgaattgtt
     2941 tatgacgatc ggtgctgtcc agttagggca gaaacagacg cagaaaaaca gcggcggaat
     3001 gaccgagctg tatctggatt ccggaaccta cgttaaaagt ttttactccg tcatgtattc
     3061 gccgtcgtgc gtaaaaatct cactgatggg tgccagccat aaacgcattc accatcaggt
     3121 cacctggaac aacgctgcag taaaaatcct tcacgaaaaa tacaggaaga agacaccctg
     3181 catatcaatg ggggtgacaa atgattccgt attcgaaagt cgagtctctg gcagcgtgcc
     3241 ggatgactgc acaacaaatc gctgacgttc tggatgttga tctgaaccga ctgaaagaaa
     3301 atcgggaagc aatgacagat ttttatgcgg ccatccgtaa gggcagagcg aaaggtgaag
     3361 ccgagttacg agcggcattg tttaagcttg ccagaaaagg ggatgccttt gctctgcgcg
     3421 aactactcag ggtggataaa aatcaggact aactgatgag cagaccggac tggggggcgt
     3481 tgcagcagga gtatattgct gaatacaccc gctccggtat atctccggtg gcatggtgtg
     3541 aagcaagggg actgaactac gcaacagccc gtcgttacat caaaaaaact ccgaaaaatg
     3601 cgcagaaaga aatgcgcaaa actgcgcaaa aaagtgcgcg aaaaaaaact gcgcagactg
     3661 cgcaaaggca gaacggaaaa tctcagaaaa aaaaggctgt atccgatgca ctcctgattg
     3721 agggggacac ggaagaaatt tcgttctgcc ccgatgaatt cggtatttct gaccagcagg
     3781 caaaattcgc catgttggtt gctcagggga aaaagccgac agaggcatac cgactggctg
     3841 gttatgaggg gcaaggtgcg acagctaaca gcaacgccag ccgtatgctt agaaatgcca
     3901 gggtttatcg tgctatcagc tacttccgca atcagtacca gaaacgctat accgcagacc
     3961 tggatttact ggtgagccag ttgatggcca ttgtccaggc cgaccccaat cagttggcac
     4021 aatttcgccg tgttaactgc cgttattgct ggggcgagaa tcatctctac cagtggcgtg
     4081 atattgcaga attcgataag gctgcggcac aggcctccag agatggtaaa cccgagccgg
     4141 aatatggagg cctcggcttt gttgataacg ccatacccaa tcctgattgc ccgaagtgct
     4201 gcggtgaggg aacaggacag ctttatatgg ctgataccac actgcttgat ggggatgcgc
     4261 ggcaattata tgcagggtca aagctcggaa aatacggtgt tgagatcctg ctggaggata
     4321 aggctgccgc ccggcgtgaa ttgttgcgtt tgctttctgc tggcggggca ttatgtgcag
     4381 ataagcggct acaggaactg gaaattgaac ggcgcagaat agagaaccag aagctgcgca
     4441 aagagatcga aacggtggag gataatgaac atccccagcc tgtggcgatc aatattaatg
     4501 tggttgatgc cagattaagg agtgatgaag atgatctctc cgacgcttaa tgtgcctcag
     4561 gcgcgatttc tttcaatgcc ccataaattt aaagcctata ttgctggttt tggctcgggc
     4621 aaaacatggg ttgggtgtgg cggcatatgc aaggggattt gggagcatcc aggtataaat
     4681 cagggatatt ttgcgccaac gtatccccaa attcgcgata ttttttaccc tacagtggaa
     4741 gaagttgctg ctgactgggg attgaacgta aaaattaatg agggaaataa agaggttcac
     4801 ttttattacg gacgccagta tcggggaacc actatctgca gatcgatgga gaagccacaa
     4861 acgatcgtcg gtttcaaaat tggtaatgcg ctggtggatg aactggacat tttgccgaag
     4921 gaaaaagcca gaacggcgtg gcgcaagata attgcgcgta tgcgttataa gattgatgga
     4981 cttcgcaacg gtattgacgt tacaaccacg ccggaaggat tcaaatttgt ctacgagcag
     5041 tttgttaaag ccgtgcgtga aaaaacagag ctggcctcac tgtatggtct ggtgcaggca
     5101 tctactttcg ataatgaaaa gaacctgcca gcagattaca ttccttcact tcttgaatca
     5161 taccctccag agctgattaa agcctatctt ctaggacggt ttactaacct gacaagtggt
     5221 actgtttacc atcagtttga ccggaaactg aataattgcg aagaagtgga gcagccaggg
     5281 gagccgattt atattgggat ggattttaac gttggaaaga tggcgggaat cgtccatgtg
     5341 ctgcgtttgg ggcttccatg tgcggtaact gaaatcatca atgcctacga tacgccggat
     5401 atgatccgcc tcattaaaga acgcttctgg ctgtatgacg ggcatgatta ccggaaagtg
     5461 agggagattt atatttatcc agatgcttcc ggagattcca gaaaatcaag taacgcaagt
     5521 acgacggata tagcccagct taagcaggca ggttttaacg ttgtggtgaa cagctcgaac
     5581 ccgccagtaa aagatcgcgt taactcaatg aatgcaatgt tctgcaatgc caatggtgag
     5641 cgtcgctata aagttaatgt gaagcgttgt ccggtatatg ccgaatctct ggagcaacag
     5701 gtctgggatg ataaggggga gcctgataaa aaatctggca atgatcaccc gaatgatgcc
     5761 ggaggttatt tcatcgttaa gcaattccct attgtcaaac cgaccggaag agtcacatca
     5821 cttcggattt aattatggct gatatatcaa cacccaacct cgactataac gatatgctgg
     5881 aggcgtggga tattaacgac gcattgatgg gcggtacgct tgaaatgcgc aggcaggggg
     5941 aaaattatct ccccaaatgg cctaatgaag atgaagacgc ttataaaaaa cgcctgtctg
     6001 tggctacgtt acttcctgtg tatgaagaaa gcatcaaaca aaatattggg cgcatatttg
     6061 cagagccgac agtattgagt gaggaaacgc cggaaaaaat cagggaatat gcagaaaata
     6121 tcgacatgga ggggagccga ctggatgtgt gggcgcagca atttttcagt ctcgcatttc
     6181 agtatggtgt ggcacatgcg ctggtggact atccacgaac ggatatgaac gagattcgga
     6241 caaaagccga tgaaaacgcg gccggtggtc gcccgtacgt tacgatgctg aatccacgcc
     6301 aggttattgg atggaaatcg aaagttaaaa aagggaaagt tgttctcact gatttgcgta
     6361 taaaagaggt catcattatt gatggtgatg atttcgggca gaagaaggtg gagcagatcc
     6421 gccatattat gccccgtcga gtagaaattt atcgacgcag cgaaggtact aatggcgaat
     6481 ctgtctggat gcttcatgag tcatggaata ccagccgtga tgatattcct ctggtaacac
     6541 tgtatacgaa gaaaacaggg tttatgcgtg gtacaccgcc attgcttaat cttggcttgc
     6601 tgaatatcaa gcactggcaa agccagagcg agcaggacaa tattcttcat gttgcccgcg
     6661 ttccattgct ggtggcctac gggctggaca ggaatgaaga actgacggtt ggtgcatcca
     6721 cggctacgat ttttgaggac agaacaaaaa atggcctcga atatgttgaa catagtggcg
     6781 cagcgataga atctggcgaa acatcacttg agaagctgga aaatcagatg cgtcatgccg
     6841 gcgctaaact tctgcgggct gaaaatacat ccaccaaatc tgttgatcag actaatgaag
     6901 agcggatgca ggaacactcg ccgctttata caatggcgaa ctccctcgaa gatgccctcg
     6961 ataatattct ccagataatg gcggaatgga gcggagagag ttgcggtggc aatgtggatg
     7021 tgcgcactga actggatgta tctgcccagg tgtttgactc atcctccgcg ctggctgttc
     7081 agtcattgcg tcagggcggt gatatacgtc agattgatgc ggttcgggtg ttgcaggcgt
     7141 tgaaatttat cgatcaggat tcccgtccag aggatgtgat cgatgaatta aaaaatcaga
     7201 gtgtaatgtt gatggaaatc aatgatgcaa accgtgaatg aacggctgcg tgatgaatca
     7261 attgctcatg cagtctggat atcccgctat agcactggtg tggctgccag aatggtgaaa
     7321 atactgaatg acagcgatgc ggagcttaca gctcgcctgc tggtagcact ggacagcctc
     7381 gatcctggta gttttaccgt tacgcgccta gagtcacttc tggcgagtgt cagagaggtt
     7441 aaccgcgctg ctattaacag catgtttacc agtctctccg gagagctgaa cgagctggca
     7501 atttatgagg ctggttatca gttaagtctg tttgattctc tgctacctga ttttgttgct
     7561 gatgttcacc ctctggttgg tatatcttct gatgcacttt acgccgctgc aatggcgcga
     7621 ccattccagg gacgactgct cagtgagtgg gccttggatc ttgaggcgga tcggctcaga
     7681 cgcataacaa atacggtgcg tcagggtttt ttgctggggg ataccaatga gcagatcgca
     7741 agaaaaattc ggggacatgt cagcaaggga tttcaggatg gtgcattgca gatgagccgg
     7801 gctaatgcgg ccagcattgc aaaaacagcg gttggacatc ttgctgctac tgcccgtgag
     7861 agttttgcca gcgcgaataa tgatttgatt aagggtaagc aatggttatc aacgcttgat
     7921 aatcgtacta cgccacaatg tcgaatcagg gatcgcctca aatatacgct ggataataaa
     7981 cctgtaggtc acagtgtgcc ttatttgcat gggccgggaa aaattcattt ctgctgccgc
     8041 tcaacggaaa cgttcattct gaaatcagcg aaggaactgg gtattgatgt tcgtgatatt
     8101 tccccggctg agcgggccag catggatggc gtggtggccg gagatacaac ctacagggaa
     8161 tggtttttgc gtcagcctta caccagacaa aaacagattg tgggggaaac ccgggcaaag
     8221 ctgattcggg atggcggtat gtcgccagat gaattttaca ccgataaagg cgaatggctg
     8281 acgctgaagc aactccgtga gcgtgatgca caggtattca gaaaagcagg gatttaaata
     8341 aatcatttat tacaacaggc taccttcggg tggccttttt tattgctgcg atccggatgg
     8401 tgagcagcgt aactgtcgga agacttaaac caggtactaa tatgaaactg aaaacggtcg
     8461 agattaacgg aaaacaatac gcagaaattg atactgctgg cctgccagtt tatgtgcacg
     8521 acgatggtaa agaaatcggc ttcgatgcac cgctggcgat aaaaaaaatt acagagctta
     8581 atggcgaggc aaaaaatcat cgcctggcta aagaagctgc agaggaaaaa ctggctaagt
     8641 ttgccgctat cgaagacccg aagaaggcga tcgaggcact ggaaatgctg tcaaaaatcg
     8701 accagaaaaa gctgatcgat gcgggacagg ttgaccaggt taaggcagaa attacgaaaa
     8761 attttcagca gcaattagat gaagaaaagc aacgctctca gatgctggag acgcagcttt
     8821 acgattctat gattggcggt agttttgcgg gttcaaaata tattgccgat aaaattgcga
     8881 tcccggcaga tttattacaa gcccgcttcg ggcaggcatt caaagtggaa gaagggaaga
     8941 tcgttgctta tgacgcttcc ggcaacaaaa tttattcccg cgcgaagcct ggcgaactgg
     9001 cgcagtttga tgaggcgctg gagttcctcg tcgaaaatta ccctcagaaa gactacatcc
     9061 tgaaagccag tggcaacaat ggcggcggct cccgtccgac acagcatgat gttggtcaga
     9121 aaacgatgaa acgctctgct tttgatgcac tggatgttgc aggtaagcaa aacgcattga
     9181 aagacggtat cacaattgtt gattaacaca tttgtcagct tccggatggg agctggtgtc
     9241 agggctggat agctcattac tccatctatt cacaattacg caaattttta aggaatattt
     9301 aattatggct ggaaataccc tgaccgggtt gatcccgact atttataccg ccctggatgt
     9361 tgtatcccgt gagcaggtag gttttatccc tgcggtagca aaaaacgcaa aagctgacgc
     9421 cgcagcaaaa gatcagacgg taaccgcgcc agttgcgcct gaggcgaaaa ccgaagatat
     9481 cgtaccgggg ccgtcagctc cgaataccgg tgatcaaaat attggcactg ttgatgtaaa
     9541 aattactaaa tccaaaatgg caccggttaa atggaatggt gaagaacaac tggctcttgg
     9601 cccttcaggg acttacaaca ccattctggc tgatcagttc aagcaggctt ttcgcgccct
     9661 tgcaaacgag gttgaggctg atcttggtgc gttgtatttc ggtgcttccc gcgccgtggg
     9721 aaccgcaggg acaacgccgt ttggtgttaa agatgatctt tctgatgctg ctctggctcg
     9781 tcaggttctg gaggataacg gcgcaccgac aaccgatctg cagatggtgc ttggctccac
     9841 tgccattgct aatttgcgcg gaaaacagtc tgtattgttt aaagtgaatg aatccggcac
     9901 tgaacagcta ctgcgtgagg gcgtgttggg gcgtctggag ggattcaata ttcacagttc
     9961 ggcaggtgtg aaacgagcgc caaaggttgc tgcaactggt tatctcgtga atggagagaa
    10021 aaaagaaggc gatgttctta tttccattga tactggttcg gggagtattt ctgcaggtca
    10081 gattgttacg ttcgctggtg atccgaatca gtatgttgtg gcagcagcga ccagcaatct
    10141 gattactctt gcagcgcccg gactgcgtca ggatctggcc gatgatactg ccatcacagt
    10201 tgttggggcc tttactgcaa atatggcgtt tgatcgcaac gcgtttctgc tggcatcccg
    10261 tactccggca atgccggaag gtggcgataa tgctgatgat gtaatgaatg ttacggaccc
    10321 gatttcaggg attacgttcc agattgcact gtatcgtcag tatcgccagg tgcgctatga
    10381 agttggactg gcatggggtg tttcatcagt gaagccggca catggttgtc tgattcttgg
    10441 ttaaacattc aaacggggct tcggccccat ttttaatggg gggcatatgg ccggattaac
    10501 taaagagcaa agagcacagc gtgaagccgc acaaaaaacg caaattgagt tagtggttat
    10561 ggtgaccgat tatcagatgt ttcccggcgc accaactatc gctaatgttc atcctgatga
    10621 agttgacaac tggaaggcta tgggctggaa aactcaggag taacacatga tcacttacgt
    10681 gacctgtgat gacgttgata atgcgtttgg gaacgcctgg acgagtgaga acgctaaaaa
    10741 taaagctgtt ttaatggcta atgcctggct taatggcttc aacctgaaaa ttaacccatc
    10801 ccgtattcct gaagaggtaa aacttgcggg agcatatgca gccagaattg cctcattcgg
    10861 taagttgttt cagcagaaaa atgattctgg cgttgttatc agtaaagccg tgtcggctga
    10921 cggggtcagt gtatcgaaat cattttctga gttgccagca aacagcactg cattgcttga
    10981 acccgattta cagcttgcga tagcactgct gaaaccgtac ggacttagtc gctcacaagt
    11041 cagggttgtg agggggggat aatggggctt cgtgaagaga ttcagtcaga ggttgccgct
    11101 gcttttgatg aggatttagc ggacgccgtg agtgattttt ctggttctta cgttacgcac
    11161 cggcactggg atcctgtgac ggaaactggc ggcgaatcca ccgcaatcta taccgggcga
    11221 ggcgtgttga cacgttataa gctgggcaga attgatggga ttaatatttt gcatggtgac
    11281 cttaaattaa ccgctctggt atgcgaggtt acggataaac cagctgtcga ccatattatt
    11341 gagatttatg atcctgtatc aaggcagtta caacgatacg aggtaattac agcaagtgta
    11401 gatccttccg catcagttta ctctatccag ttaaggagag cgtaaatggc aaaggcatgg
    11461 gatatagaac cgtcaatatt tgccgggatg attgaggaag atgtagggct gaagattcgc
    11521 tacatcgcta ttcagattct tactgctatt gatattgctg ctccggttga taccggacgt
    11581 ttcagaaata acaacctggt gtcgttacag catcccgatt ttggtatatc tgataacgtg
    11641 gatccgaacg gtacgattgc tgttcagcgt gggatcgggg ttatttcgaa agctgcaaat
    11701 tacggggtta tttatatcca gaataacctt ccttatgcag aggctcttga aaacggtcat
    11761 tcacaacaag cgccaactgg cgtgtatgcc aacgcttttc atggtgtttt acaggcttac
    11821 aaatgaagtt tactgaaatc agaaatacgg ttatttccag aatgacggca cagacgatta
    11881 ttgatggaaa agacgtattg tatccgaacg ggccaacgtt cgatccttcc ggtaagttaa
    11941 tctgggcgcg gttaagtaat attcccgggc aggctggagt taatgaaatt ggcgcggggc
    12001 cgattgttta tcgcacgggg ataatcatta ttcagttatt tgtccccgca ggttctggtt
    12061 caaaactcat tactgagaca gccgataaat tgcgggaact gtttgagttt caggatgacg
    12121 atcgtctcag ttaccaggct gtttcctcaa tagccgttgg cgaaaagaat ggctggttcc
    12181 agcttaatct tcaaattcca tatcgcgcgc tctagcgcaa ttaatgacat aggaggctcc
    12241 tgtgagttca ggtgcaaagg ttatctcggc atttatccgg gagacggttg caggcactac
    12301 gccagcaagt ggtgactgga gtttattaaa acgcacaagc tggggagtaa aacccaccca
    12361 gaataaaggc gaaaataatg agattggtgg ctcccggatg gctcaggggg cgacgcctgg
    12421 cactgtggat gtcggcggtg atgttggtac caaatttcgc tggggtcaac atgatgattt
    12481 tcttgcatcc tgtttcggcg cggaatggtc aggcgattct ctgacaatgg ggaatgagcg
    12541 aataacattt tctctggcga cctatgcgtc cgatgtcgga attgcctctg tcgtcagagg
    12601 agcgcaggtt ggctcatgga aaatgcagat ccctaacgac ggcgatatta cagcgaccgt
    12661 aacctttgcc ggactggact gggaatcaaa ggccgatgat acgaatttta tcaaaggcga
    12721 acctgtggat agtgcaggaa agctacgtta ttcgtttaag gaggtttcag cagtaagcct
    12781 gaatggtgtt gccggaggta acggtttttg tatcgacagt tttgatattc agttcgataa
    12841 caaactccag acacagcgtt gtatcgggac tggctcgcct tatgcaggag caaatattcc
    12901 gactactttt acaccgtccg gtacggtgac gctttcatgg tctaaagccg cgtgggaaat
    12961 ctggagtaaa acactgactg gagaaacagt tccgttcagc ttcacgcttt cgaatggaga
    13021 gggggcatac actttcagtt tcccgaaggt tcaggtgtca ggtgaatggc ctgatggggg
    13081 taatagcgac attatccagg ttcaattgag cattaccgca gcagatgaag cacctacgat
    13141 aaccagaaaa aaaaattccc cggctgccgt gatcgcaaaa gccagtgctg aggcgattag
    13201 ttgattttcc gttattcctc ctgtggtact gccctacagg gggacgcatt gaatgaggtt
    13261 atggatgttt attcttaatc agaaaattat cattggtgga gaacgctggt ttacgccaat
    13321 gaaggactta aaacctgtag acgggttaaa actgttggtg gcaagcagcg ataacgatca
    13381 gtatcgctcc cgtaatgcat taatccgtcg ccacattgag aaaatggatg ccagtttgca
    13441 cgtcggaacg aaggagtttg atatttcaaa ggtttccgag gtggattctg ttgatgattt
    13501 actcattgat aatgccgctc gttatctgct gaaaga
//
LOCUS       DAEVZA010000134        13424 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEVZA010000134 DAEVZA010000000
VERSION     DAEVZA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.134"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            84..227
                     /gene="mgrB"
                     /locus_tag="LAF92_004023"
     CDS             84..227
                     /gene="mgrB"
                     /locus_tag="LAF92_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBI9792946.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            302..589
                     /locus_tag="LAF92_004024"
     CDS             302..589
                     /locus_tag="LAF92_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBI9792947.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            1259..1402
                     /locus_tag="LAF92_004025"
     CDS             1259..1402
                     /locus_tag="LAF92_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBI9792948.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            1415..1624
                     /gene="cspE"
                     /locus_tag="LAF92_004026"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             1415..1624
                     /gene="cspE"
                     /locus_tag="LAF92_004026"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBI9792949.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            1790..2599
                     /gene="rlmA"
                     /locus_tag="LAF92_004027"
     CDS             1790..2599
                     /gene="rlmA"
                     /locus_tag="LAF92_004027"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBI9792950.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(2596..3162)
                     /gene="mntP"
                     /locus_tag="LAF92_004028"
     CDS             complement(2596..3162)
                     /gene="mntP"
                     /locus_tag="LAF92_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBI9792951.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(3591..4049)
                     /locus_tag="LAF92_004029"
     CDS             complement(3591..4049)
                     /locus_tag="LAF92_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBI9792952.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(4104..4955)
                     /gene="manZ"
                     /locus_tag="LAF92_004030"
     CDS             complement(4104..4955)
                     /gene="manZ"
                     /locus_tag="LAF92_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBI9792953.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(4968..5768)
                     /gene="manY"
                     /locus_tag="LAF92_004031"
     CDS             complement(4968..5768)
                     /gene="manY"
                     /locus_tag="LAF92_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBI9792954.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(5831..6802)
                     /gene="manX"
                     /locus_tag="LAF92_004032"
     CDS             complement(5831..6802)
                     /gene="manX"
                     /locus_tag="LAF92_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBI9792955.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            7265..8821
                     /gene="yoaE"
                     /locus_tag="LAF92_004033"
     CDS             7265..8821
                     /gene="yoaE"
                     /locus_tag="LAF92_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBI9792956.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(8825..10423)
                     /locus_tag="LAF92_004034"
     CDS             complement(8825..10423)
                     /locus_tag="LAF92_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI9792957.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(10554..11918)
                     /gene="sdaA"
                     /locus_tag="LAF92_004035"
     CDS             complement(10554..11918)
                     /gene="sdaA"
                     /locus_tag="LAF92_004035"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBI9792958.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(12102..12680)
                     /locus_tag="LAF92_004036"
     CDS             complement(12102..12680)
                     /locus_tag="LAF92_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBI9792959.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(12684..>13424)
                     /gene="pabB"
                     /locus_tag="LAF92_004037"
     CDS             complement(12684..>13424)
                     /gene="pabB"
                     /locus_tag="LAF92_004037"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBI9792960.1"
                     /translation="DCYQVNLAQRFHATYSGDEWQAFLQLNQANRAPFSAFLRLEQGA
                     ILSLSPERFILCDNSEIQTRPIKGTLPRLPDPQEDSKQAEKLANSAKDRAENLMIVDL
                     MRNDIGRVAVAGSVKVPELFVVEPFPAVHHLVSTITARLPEQLHASDLLRAAFPGGSI
                     TGAPKVRAMEIIDELEPQRRNAWCGSIGYLSFCGNMDTSITIRTLTAINGQIYCSAGG
                     GIVADSQEEAEYQETFDKVNKILRQLEK"
BASE COUNT         3414 a         3288 c         3341 g         3381 t
ORIGIN      
        1 agcacgaata tcgacatagt taggcgctgt ttaactaacg catgctagtt taatgacata
       61 aggtaggtga agcggagatt ggagtgaaaa agtttcgatg ggtcgttctg gttgtcgtgg
      121 tgttggcttg cttgctgctt tgggcgcagg tattcaacat gatgtgcgat caggatgtac
      181 aatttttcag cggaatttgt gccattaacc agtttatccc gtggtgatat cattttttca
      241 gcactagatt ttcttcctct ccagcagtgg tagaatagcg cctctactca gaggtggtga
      301 aatgaacgaa gttgtaaatt caggcgtgat gaacattgcg tctttggttg tatcggtggt
      361 ggttcttctt atcgggctca tcttgtggtt ttttatcaat cgtgccagtt ctcggactaa
      421 cgaacagatt gaactgcttg aggcgttgct ggatcagcaa aaacgtcaaa atgcactgtt
      481 acgtcgtttg tgcgaagcaa acgaacctga gaaagcagat aaaaagaccg ttgagagtca
      541 aaaatcggtt gaagacgaag atattattcg tctggtcgcc gaacgataat ccttcctcaa
      601 tcttgagtaa ccccgtcgtt gaagggttac tcaattcatc ctgattatac gcactgccat
      661 ggtgcaactg cttcaaatta aaactctggt ctgacttcaa ataattatca tattatttat
      721 ttgcgtaacg ggatatcaga aaaagatatt ttcctcatta atttattagg atgtttacat
      781 cggatttgtg attaagcgtg gtattattta ttagcgaaac gtttctctct tgattttttt
      841 gctcattcat caatttttct tattttaaat tttcaatccc tttggggatt gacttctctt
      901 tagggtaatt gatagccgtt aactgactgt tttatgagaa aaagtgatat aactttttat
      961 tcattgcata gcaaaaaatg tgatattgca cgcactatgt aataacttct cccactggcc
     1021 tggaacaact gaacttattg aactatgtta gaaaatacgc cagtttaagt atctgcctga
     1081 actggcaagg ttaagcacaa tgatatatcg gcgcgtattc cgttgcataa gtgtgcaaaa
     1141 aaagtggaag acgtatcgag atttgtgcgt ctgatcgaga catgtttaaa aatggcttgc
     1201 cataattaac gttgtatgtg ataacagatt tcgggttaaa cgaggtacag ttctgtttat
     1261 gtgtggcatt ttcagtaaag aagtcctgag taaacacgtt gacgttgaat accgcttctc
     1321 tgccgagcct tatattggtg cctcatgcag taatgtgtca gttttatcta tgttatgcct
     1381 gcgggcgaag aaaacaatct aaggaatttt tcaaatggca aagattaaag gtcaggttaa
     1441 gtggttcaac gagtctaaag gttttggctt cattactccg gctgatggca gcaaagatgt
     1501 gttcgtacac ttctccgcta tccagggtaa tggcttcaaa actctggctg aaggtcagaa
     1561 cgttgagttc gaaattcagg acggccagaa aggtccggca gctgttaacg taacagctat
     1621 ctgatcgaat ccactgatct gaagtgtgaa tacgcttcaa tctcgctata aagcctcgtc
     1681 gaatgcgagg ctttttacta tgctttatct tcgctcctgg cgttcggata tttgcccgcc
     1741 gcgtgattcg cgttacactt gcggccttta gtatcctgcc ggagttgtca tgtcgttttc
     1801 ctgtccactt tgccatcagc ctctttcgcg tgaaaaaaac agctatatct gtccccagcg
     1861 acatcagttt gatatggcga aagaagggta tgtcaatctg ctgcccgttc agcataaacg
     1921 gtctcgtgat ccgggcgaca gcgcggaaat gatacaagca cgccgcgcat tcttagatgc
     1981 cggacattat cagccgctgc gtgatgcaat tgtcgcccaa ctgcgggaac ggcttgatga
     2041 aaaggccacg gcggtgctgg atattggctg tggtgaaggg tattacacac acgcatttgc
     2101 cgatgtgttg cccgaaatca ccacgtttgg cctggatgtc tcgaaggtag cgataaaagc
     2161 ggcggcgaaa cgctatccgc aggtcactct ttgtgtcgct tccagccacc gtttgccgtt
     2221 ttccgatacc agtatggacg ccataatacg tatttacgcg ccgtgtaaag cagaagaatt
     2281 agcacgagta gtgaagcccg gcggctgggt cattactgcc acgccgggac cgcgacattt
     2341 gatggagctg aaggggctga tttacaatga agtacatctt catgcacctc atgcagaaca
     2401 actggaaggt tttacattac agcagagtga tgagttgtgt tatccgatgc gtcttcgcgg
     2461 tgatgaagcc gtcgcattat tgcagatgac gccgtttgcc tggcgtgcga agccagaagt
     2521 ctggcaaaca ctggcagcaa aagaagtgtt cgactgccag acggacttta atattcacct
     2581 ctggcagcgt tcttattaac cgtggaagtg cgtccagagg atctggacgc cgatgccgat
     2641 cagcaccagc ccgccgagaa tttccgcttt tttcccaata attgagccga taaagcgacc
     2701 aaccatcatc cctaatgtcg acataatcaa ggttgcacaa ccaatggcca atgcggtcgc
     2761 gataatgttg acctgcagga aagcaagacc aacacccaca gccatggcat ccaggctggt
     2821 ggcaatcgcg gtggttacca gtagccagaa accgtgccga cggcgcggct cttcatcttc
     2881 atcatctgcg ccacgaaaac cctcaataat cattcgcccg ccgaggaata tcagcagcac
     2941 aaacgcaatc cagtggttcc attcaaggac aaaccggctg gctaacatgc ccattcccca
     3001 gccgatcagc ggcgtcaggg tttcgacggc accaaaaata aggccggttc gcaatgcttc
     3061 agaaaatttc ggtttatgga gggtggcacc tttaccgatt gatgcagcaa atgcatccat
     3121 cgacatacca aacgcaagaa gaacagtagc agtgatattc atgacaatgt cctgaccggg
     3181 gtatccataa aacacatcat cgcccccagc aaacatgcat acatgaacaa caagccgtga
     3241 tggttttcgc gttcatgcag gcatagcgat tgatgtgcat atggtctcgc ctgactgaaa
     3301 gtattcagtc cgtacgtgcc acgttttgca acgagtatgt tgacacgtac atttccggaa
     3361 tctggaaatc agctactccc caaatgaggg cgcaacctta acatattttg agaatataaa
     3421 acaactgttg gaatatatta tttcattgct ttattgataa cgattttcat ttagatttat
     3481 atatcaatga aacgttaaaa taaataacaa ataagggaag agataatata gcctaagcta
     3541 tgtctgatta ttaaaaatac actaaacata gctttggcta aattcataag ttattgagtt
     3601 gatacgagaa gcttataaat tttttccaga tcgtcgatat ttcgaacgcg gattaacagc
     3661 cgacgctgtt ctaattgcat caccaacacg ccatcttccg acaagttcat cgctttgatt
     3721 cggctatatt caatccagac attggcgaag aaaaaacctt tttgtttaaa gatgatcttc
     3781 ggaacgcgga tccagaatat ataaaaaccc atcagagcca gtgcacttaa taaccatgtt
     3841 gttattagcg cgccatgatt cgtgacgttg ttatagataa gaatgacaat caatccgacg
     3901 aagataacgc tatcgatgcg accacgccgg agcaaaggaa ttgccagcag ggtggggccg
     3961 ttacggcggg gcatgatgaa ctgatcgtag atcgcgaagg ccaggagtgc ggcgatgaaa
     4021 agaatcagta ccaggtccgt gattgtcatt aatcctccag ataaaaaaac ggggccaaaa
     4081 ggccccggta gtgtacaaca gtcttacagt cccagcaggc cgcaagcgta accagcgata
     4141 ccgatgacga agaagccaac gatgatccac agcgggttaa cttttttgcg cagcagccac
     4201 atacaagcaa aggtcagcag cagtggtacc aggcctggca ttaactggtc aagaatggtt
     4261 tgcacggtgg tgacacgggt ctgtccatcc tgcccggtga tggtcgaaac caccagcggg
     4321 atgttgacat gtgtccactt gttaaccaat gcccccatga caaacaggcc gaggatagac
     4381 gccccttccg tcagtttttg caggaagcca ccacccatat ctttaacgat atcgatacct
     4441 ttggagtaac catacgctac gccgtagtaa cgggttgcca gacgcaccag gttaaacagg
     4501 atgaagaaca gcagcggacc taacaggctg ccgctcatcg cgataccggc acccagtgct
     4561 gcaaataccg gacgtacggt tccccagaag atcgggtcgc ctacaccagc cagcggcccc
     4621 atcaaaccga ctttgatacc gttgatagca ccgtcatcga tctctgcgcc attagcacgc
     4681 tgttcttcca gcgccagggt tacgccgaga atcggcgcag ccacgaacgg ctgggtgtta
     4741 aagaactcca ggtgacggcg aatagcttgt ttacgagctt cgttgttctc agggtagagg
     4801 cgacgaattg ccggtaccat agagaagcag aaacccagtg cctgcatacg ttcgaagttc
     4861 catgaaccct ggaagaggtt agaacgcagg aagacgccac gaatatcact ttgagtgagt
     4921 tttttctcgg tggtagtttg agttgtatca accatttcgc tcacctgtta gtccagttcg
     4981 ttatcgagat cgttgttacc agctgcctga gcaggcgcac cggctacgcg gttgtatttc
     5041 gggctaagtt ggatgtagag cactgccata acagtaccaa tcacacccag agcaaccagg
     5101 ttaaagttgg tgaatgctgc ggttacgaag ccgaggtaga agaacggcat caggtagcca
     5161 gcacgcatca tgttgataac catcgcataa ccaaccacca cgatcatgcc accggcgata
     5221 ttcagaccat tggtcaccac ttccggaatc gcattcagca tgttctgtac ttcgctggta
     5281 ccaacagaca gcgcaacgat gacggccgga atagccacac gcattgcttg caggaacaga
     5341 gaagaaacgt ggatccagga aatcgctgtc aggttgccgt tatcagcagc cttatccgca
     5401 gcgtgctgga aagcaacggt aatagtacga acgatgatgg tcagtacctg gcccgcagcg
     5461 gccagaggga ttgccagtgc gatacctgca ccaatgctct gatgacccgc gataaccaga
     5521 atggtagaaa tgatagaagc cagagcggcg tcaggcgcaa ctgcagcacc gatgttcatc
     5581 cagcccagcg cgatcatttc cagcgtacca ccgataataa taccggtttt catatcccca
     5641 agaacgatac ccaccagggt acacgcgatt agcggacggt gaaactgaaa ttcatcgagg
     5701 attgatccca tacctgcgat acaggctacg ataaatacca gcacaatttg aagagtggta
     5761 atctccattg tacttctcct gtttacgtaa gacttaagtg tgaaaactga gtgataatca
     5821 acacaatacg ttacttatcg attttgctga tcagatccat cattttcagt ttcggatcgg
     5881 tggaaacctt acggacttcc agctcaatac cgcgcgcatt cagtttcttg aacgcctcga
     5941 tatctttttc atcaaccgaa accgcgttat tcacctgggt tttaccctga cggaatgcca
     6001 taccaccgac gttaacagag gtgattttca cgccgccttc aacgagacgc tctacatctg
     6061 ttgggttggt aaataacagc attacgcgtt cgccagcata tttcgggttg ttgtagacgc
     6121 gaatcatttt ggcaacatca actacgtgtg ctgttacgcc cggaggtgca acctgggtga
     6181 gcagtgtctt acgaacggta tccgcagcca cttcatcact aacaacaata atacgggaga
     6241 cattggtttc tttggtccag cgggtggcga cctgaccgtg aatcagacgg tcgtcgatac
     6301 gcgcaaggcc aataaccatg tagtcgtttg gccccattgg ttttgccgga gttggagccg
     6361 cttttggtgc tgcggcagcg ggtgctggcg cggctttttc aaccggtttg gctttcagag
     6421 ctttcacgcc ttcacggcct gtttctactg ccagtgccac cagttcatca aagcttgggt
     6481 catcatcacg ggccattaac gtttccacga gcattggaat gttaacgcct gcaatgactt
     6541 cataatgctc tttgtcgacg acaatgcggc tggcagcatt gaacgggctg cctccccatg
     6601 tatcaacgag aaacagcacg cctttagtgg tgtcgagttt tgccaactga gcgttgtact
     6661 tttcaatcag cgtttcggca ttttcacctg gaacgaaatc gatccagccg acgttttcct
     6721 gctcgcctaa cagcatttct gccgttttaa gcaactgctc tgcagcccaa ccatgtgtgc
     6781 ctataacaat agcaatggtc acttgctacc tcctttatta tcgttaacac ctcaacgtgc
     6841 cagatgtatt tttgaatcgt cgtccacaat cgaatcgatt cagataaggg ctacaaccaa
     6901 aaacccctgc gtttcgcgat ttattttaga tatcgaaaaa attattttat gtgatgaaga
     6961 tccgtaattt aactttcgat tagcagaaat ttcgaaaggt aaaatatcct tgtcacattc
     7021 gtttgcaaag gaaggtaaat ctttgccaaa tcagaggcgt ctctgatatg tttaactccc
     7081 gtttaacaac catggagtat agggcagtag cccgcagtat ggatcgtcac cgacgtcatt
     7141 tcagcatcag gccttttaac gcctaccttt ctggctctct atgccgcacc tttcgtttgc
     7201 attttgtcgt tacgcctgca ttatttctgg cgtcgaatag ctattcctta agcaggagct
     7261 tgtcatggaa ttcttaatgg acccctcaat ttgggcgggg ctactcacgc ttgttgttct
     7321 cgaaattgtg ctgggtatcg ataacctggt cttcatcgcc attcttgctg acaaactgcc
     7381 gccaaaacaa cgcgataaag cgcgtttgct ggggttatca ctggcgctga ttatgcgtct
     7441 ggggctgctg tcgctgattt catggatggt cacgctgacc aaaccgctat ttaccgtcat
     7501 ggatttctcc ttctccggac gcgacctgat tatgttgttc ggggggatat tcttgctgtt
     7561 caaagcaaca accgaactgc atgaacggct ggaaaaccgc gatcatgatt ccggccacgg
     7621 taaaggctac gccagtttct gggtggtcgt cacacagatt gtcatccttg acgccgtctt
     7681 ctcgttggat gcggtaatta ctgcagtagg gatggttaac catctgccgg tgatgatggc
     7741 ggcggtagtg attgcgatgg cggttatgtt gctggcatcc aaaccgctga cgcgattcgt
     7801 taaccagcac cccacggtgg tggtgctctg tctgagcttc ctgttaatga ttggtctgag
     7861 tctggtggca gaaggtttcg gtttccacat tccgaaaggt tacctgtatg ccgcgattgg
     7921 cttctcgatc atcatcgaag tgtttaacca gattgcgcgt cgcaacttta ttcgccacca
     7981 gtcgactttg ccgctgcgag cgcgtactgc cgatgccatc ctgcgtttga tgggcgggaa
     8041 acgtcaggcc aatgttcagc acgatgccga taacccgatg ccgatgccga tcccggaagg
     8101 tgcatttgcc gaagaagaac gttacatgat taacggcgta ctgacgctgg cgtcgcgttc
     8161 tctgcgcggg atcatgacgc cgcgcggtga aataagctgg gttgacgcta atctcggggt
     8221 agatgaaatc cgcgagcaac tgctctcttc accgcacagt ctgttcccgg tatgtcgcgg
     8281 tgaactggat gaaatcatcg gtattgtacg tgctaaagaa ctgctggtgg cgctggaaga
     8341 gggcgttgat gtggcggcga ttgcttcggc gtctccggcg attatcgtcc cggaaaccct
     8401 cgatccgatc aacctgttgg gcgtgctgcg tcgtgctcgc gggagctttg ttatcgtgac
     8461 caacgagttt ggtgtggtac aaggtctggt cacgccgctg gatgtgctgg aagccattgc
     8521 gggtgaattc ccggacgctg acgaaacgcc ggaaatcatt actgatggtg acggctggct
     8581 ggtaaaaggc ggtacagatt tgcatgcctt gcagcaggcg cttgatgttg agcaccttgc
     8641 cgatgacgat gatatcgcga cggtcgcggg cctcgtgatc tcggcaaatg gtcacattcc
     8701 ccgtgtgggc gatgtgattg atgtagggcc actgcatatc accatcattg aagccaatga
     8761 ttatcgtgtt gatctggttc gcattgttaa agagcaaccg gcgcacgatg aagatgagta
     8821 agcattaacg taacggcata atgggcgtga tatgtccatt atgccgggcg ggcggcggtt
     8881 ggctgcccgc cagccatttg ggaaaatcac gtagcggcat cggctgtgcg tacaaatacc
     8941 cttgcaaaat atgcacgcca tgacggcgca aatacttcgc ctgttcttgc gtttccacgc
     9001 cctccgccac cagttcaata ttcagtcttt gccccagcgc gatgatgata tcggtcaccg
     9061 tcgagttaac cgcgtcagaa cctatagctg cggtaaatga cttatcaatt ttcagcacgt
     9121 caggacgtaa tgtttcaagc caggaaaatg aactgttgcc ggtgccgaag tcatcaatcg
     9181 ccagtttgac gtttttacga tgtagctcgc gggcaatccg ataatcaaca tccagtaagg
     9241 catcgcgttc ggtgatttcg aggatcagtt gctgaatcgg gtgagcgcta aaccagtact
     9301 gattgagatc ttttatcagc acaccacggc gaaaatggct gggtgcgacg ttaatgccaa
     9361 catgaaactg actactcatc gggaaaacat ggcgctgacg aatggtttct gccatcacat
     9421 agcgggtcag tggcacaatt aaatgatgtt cttccgcgat aggaataaac acatccggtg
     9481 aaatccagcc ctgacgcgga ttattccagc gcagcaaaat ctctacacca atacattgct
     9541 ggctgcgcgc attaagcaaa ggctgacaga acaattcgaa ctcatgttgc gccaggccca
     9601 gattgatttc gcgggaaaag ctcatccggt aagcggtcgc cagccaggca atgtagccca
     9661 ccagtaaact tagcagcacc gccagcggta attgtgttgg aagatagtgc catgccagcg
     9721 ccgtagcccc cggaccatta acgttaatgg taaaaggaaa gcgttgcgaa gaaacctgat
     9781 agttttcatt gtcttcaggt tgcggaaggg aatctaccag cccattacca tagagcaaat
     9841 gccgtttgtc caccgtcagg ctggcgctac tgatttgcgg cagttgtggc tcaaggagca
     9901 ttgccgtcag taagtcgatg ttaatcatct ccatgacccc agcattgcta ctgcccgctg
     9961 caggcgtcca ttgaatcaaa accggactgc ctttaatcag ggcacggtcg atcgtcaggc
    10021 gtaaaagtgg ttgcggtgca ggaagttcag ccagaatgtc cacgacgggc acattgcgat
    10081 aaccaaaaat gctggagcaa tagagtgtgc cgtcttgcac caggccaatg gatcgcacag
    10141 tttggagttt tgccgcctgt ttacgtaatg gcaaatgggc gacagagcag ggcagaccaa
    10201 tcagcggaag caagacttcg ctacctgcct gtaggggaag cagtacttta tctaattcct
    10261 ctacagcgtg attggcgaat tgtactaccc gttgttgatt taagttacgc tgtgaaataa
    10321 atcgcacgct cagggtcgaa gcgagcgtaa cgagtgcgca aatcgtacaa acaatcattc
    10381 gattacggcg ataggtttta atgatccgtt gtgctttttg catgcgggcc accctgtaag
    10441 cgtatggcga caaacgcctg acgcaacagt ggaagtgtag tggggaatat tacagcagac
    10501 gagaaagcgg gtataaattc gcccatccgt tgcagatggg cgagtaagaa gtattagtca
    10561 cactggactt tgattgccag accaccgcgt gaggtttcgc ggtatttggc gttcatgtcc
    10621 ttaccggttt cgtacatcgt ttcgatgacc ttatccagcg agacgcgcgg tgcactggtg
    10681 cggcgcagag ccatccgtgc ggcgttaatc gccttcacag aggcaatggc attacgctca
    10741 atgcacggca cctgaacctg ccctgcaacc gggtcgcagg ttaaaccaag gttgtgttcc
    10801 atgccaattt ccgccgccac gcaaacctgt tccgggctac cgcccagcag ttctgcaaga
    10861 cccgcagcag ccattgaaca ggcaacaccc acttcgccct ggcaaccaac ttccgcaccg
    10921 gaaatagagg cgttcatttt atacaatgca ccaatcgcgc ccgctgccat aaagtaacgg
    10981 gtatagatgt ccgggctgac cgattcaata aagtggtcat agtaagccag cactgccgga
    11041 acgataccgc aggcaccgtt ggttggcgca gttaccacac gaccaccggc ggcgttttct
    11101 tcgttaactg ccagcgcaaa catgtttacc cagtcaatga cattcatcgg atcgttagac
    11161 agtttatcgc tggaaactaa catccggcgc agggcagacg cacgacgtgg cacgcgcagc
    11221 gggcctggca gtacaccttc ggtgttcatc ccgcgatcga tacatgcctg catggtttgc
    11281 cagacatgcg cgaaatactc gtcgatctct ttcttgctgt gcagcgccag ttcgttctgc
    11341 atagcgagac cagacagcga atagccggtt tcattacagt acgcgagcag ttcggtggca
    11401 gatttgaacg gatacggcac gctcacttcg ttggcagcat cctgaccaaa gtgttcttca
    11461 tcgacgataa aaccgccgcc gatggaataa taagttttgc tgtagacgac ttcatcgccg
    11521 ttataggcgt ggatttgcat accgttttca tgcagcggca ggttgccgtt atgaaaacgc
    11581 atcccgttgt cgcgcgggaa atccacttca tgccgtccct gtgccagcag cagacgttcg
    11641 cgctcttcta cgtcgcgaat aaaaccggga atactgtcga tatccacggt ggcaggttcg
    11701 ttacctgcaa gacccataat aatggcgata tcggtgtggt ggcctttacc cgtcagcgac
    11761 agtgaaccat aaacgtccac ggcaacgcga gtaacgctat ccagtaagcc tttttcgacc
    11821 agatcatcga cgaactgttt acccgccttc ataggcccta cggtatggga agatgaggga
    11881 ccaatcccca ccttaaacat gtcgaataga ctaatcacga taatactcct gacaaggtga
    11941 ctggacttcc agtaacgaac taataacagc gcatagtgta agagggaacg cggcgactgg
    12001 cttaactatt cacatgaatt aaactaatca aaaaccgcga tttaactaat attgcgatga
    12061 ttgtctcaag tagtgttttg tagatgtaaa gataagtata gtcagggttt cacaccaatt
    12121 tgcagcgcca gctcacgaat tatgcctgcg gtcattcccc atacaaaata ctgttcgtac
    12181 caggacagcc atacccgatg tgaatcgcca cggcggtaga tatctaaagg gtgataacga
    12241 cccagatgta atgcctgggc gagcggcatt tcaaacaccg ccgagacttc atcttcactg
    12301 gcgcgatacg gcagatcggg cgggataatg ccgaccactg gggttacctg atagccagtt
    12361 acgctatcga cgggcggcag cacgccgata acttcaacgg cggaaggcgg tatagcgacc
    12421 tcttcttcag cttcgcgcag cgcggcggcg ataactgatg cgtccgtgtc atcgactgca
    12481 cctccaggga atgccacttg tccagcgtgt ttacgcagat gaattgaacg ctgagtcagc
    12541 aacaaccccg gttgcggtcg acggacgatg gggattaaca cggcagcctg acgatgattt
    12601 agggtttccc ggttaatttg cgggcgcaaa agctgaaagc gagataaaaa atcatcaagc
    12661 gtcaggctac ggtattccac gtcttacttc tccagttggc gtaatatctt attaacttta
    12721 tcaaaagttt cctgatattc cgcttcttcc tggctatcgg cgacaattcc accgcccgca
    12781 gagcagtata tttgtccgtt aatggcagtc agcgtgcgga tagtaatgct ggtatccatg
    12841 ttgccgcaaa agctcaaata gccaatgctg ccgcaccagg cattacgtcg ctgcggttcc
    12901 agttcgtcga taatttccat agcccgtact ttcggagccc cggttattga gccaccagga
    12961 aaagctgcgc gcagcagatc gctggcgtgt aactgttctg gtagtcgcgc cgttatagtg
    13021 ctgaccagat gatgcacggc agggaagggt tccaccacga acagctctgg tacttttacc
    13081 gaaccggcta cggcaacacg accgatatca ttacgcatta aatcgacaat catcagattt
    13141 tcggcacgat ctttcgctga gttcgccagt ttttctgctt gtttgctatc ttcctgagga
    13201 tcgggcaggc gtggtagcgt gcctttaatc gggcgggtct ggatttcact attatcacaa
    13261 agaataaacc gctctggcga aaggcttaaa attgcaccct gttcaagacg taaaaaagcg
    13321 ctaaatggcg cgcggttggc ctgattaagc tgaaggaatg cctgccattc atcgccagaa
    13381 taggtcgcat gaaaacgctg ggcgagattc acctgatagc aatc
//
LOCUS       DAEVZA010000135        13177 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEVZA010000135 DAEVZA010000000
VERSION     DAEVZA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.135"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(273..1070)
                     /locus_tag="LAF92_004038"
     CDS             complement(273..1070)
                     /locus_tag="LAF92_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI9792961.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(1081..2085)
                     /gene="holB"
                     /locus_tag="LAF92_004039"
     CDS             complement(1081..2085)
                     /gene="holB"
                     /locus_tag="LAF92_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBI9792962.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(2082..2723)
                     /locus_tag="LAF92_004040"
     CDS             complement(2082..2723)
                     /locus_tag="LAF92_004040"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBI9792963.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(2713..3735)
                     /gene="yceG"
                     /locus_tag="LAF92_004041"
     CDS             complement(2713..3735)
                     /gene="yceG"
                     /locus_tag="LAF92_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBI9792964.1"
                     /translation="MKKVFLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(3738..4547)
                     /gene="pabC"
                     /locus_tag="LAF92_004042"
     CDS             complement(3738..4547)
                     /gene="pabC"
                     /locus_tag="LAF92_004042"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBI9792965.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYNRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSAGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(4667..5908)
                     /gene="fabF"
                     /locus_tag="LAF92_004043"
     CDS             complement(4667..5908)
                     /gene="fabF"
                     /locus_tag="LAF92_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBI9792966.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(5996..6232)
                     /gene="acpP"
                     /locus_tag="LAF92_004044"
     CDS             complement(5996..6232)
                     /gene="acpP"
                     /locus_tag="LAF92_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI9792967.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(6443..7177)
                     /gene="fabG"
                     /locus_tag="LAF92_004045"
     CDS             complement(6443..7177)
                     /gene="fabG"
                     /locus_tag="LAF92_004045"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBI9792968.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(7190..8119)
                     /gene="fabD"
                     /locus_tag="LAF92_004046"
     CDS             complement(7190..8119)
                     /gene="fabD"
                     /locus_tag="LAF92_004046"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBI9792969.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(8135..9088)
                     /gene="fabH"
                     /locus_tag="LAF92_004047"
     CDS             complement(8135..9088)
                     /gene="fabH"
                     /locus_tag="LAF92_004047"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBI9792970.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(9156..10226)
                     /gene="plsX"
                     /locus_tag="LAF92_004048"
     CDS             complement(9156..10226)
                     /gene="plsX"
                     /locus_tag="LAF92_004048"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBI9792971.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(10307..10480)
                     /gene="rpmF"
                     /locus_tag="LAF92_004049"
     CDS             complement(10307..10480)
                     /gene="rpmF"
                     /locus_tag="LAF92_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBI9792972.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(10532..11053)
                     /gene="yceD"
                     /locus_tag="LAF92_004050"
     CDS             complement(10532..11053)
                     /gene="yceD"
                     /locus_tag="LAF92_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBI9792973.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            11252..11836
                     /gene="yceF"
                     /locus_tag="LAF92_004051"
     CDS             11252..11836
                     /gene="yceF"
                     /locus_tag="LAF92_004051"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBI9792974.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(11948..12907)
                     /gene="rluC"
                     /locus_tag="LAF92_004052"
     CDS             complement(11948..12907)
                     /gene="rluC"
                     /locus_tag="LAF92_004052"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBI9792975.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         3198 a         3618 c         3143 g         3218 t
ORIGIN      
        1 gggtgctttt tttacgttct cacgcgtggc aaggggggag agcctcgccg tgtacagggc
       61 atctaagcgc cctttattta ttacacagag taaaataatt cagtgccaat ttgtttgacg
      121 gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa aacatatgtc
      181 acaatatttc aaacgtggtt attcaccgcc caattttcgg cggtgaactt tactcgtttt
      241 agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag gcgggaagcg
      301 tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc ttcaacggca
      361 acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg ttgattctct
      421 tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag taatcgatcc
      481 agggggacat aacgcgcagc atcgcgcagt tgctccgcat tacggaaggt cacaatgccg
      541 gaaaaggaga tgtaaaatcc gagatccagt aatttacccg ccgtttctct gtcctctgta
      601 aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat tgccagcgta
      661 tcggcacggg cgtcacgggt atggacgata accggcttgt tcagttcacg accaatctgg
      721 atatgatgga tgaaggactc ttgctgacgt actttagttt ccggtgtgta ataataatcc
      781 agcccggttt cacccagcgc tacaacaccc tcttctgccg ccagacggcg taaatcttct
      841 acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac gacgttgtca
      901 cgttcgccta ccagatctcg catatgtaaa taacccggta atgttgtggc gactgccaga
      961 caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc cttatgcaaa
     1021 gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat gatgtctctc
     1081 ttaaagatga ggaaccggta gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa
     1141 aagatcggtg atgagaagct cgcggttgat gcctgtaaca gacattaact gttcacgaat
     1201 gtggcaaaca tcccccagta tagcctgcag gcgcgaggga gaaagatggt ttgccagttc
     1261 ggcgaccagg cccggcacat caacattggt cacctgcgca gcaccatgat ggcgttttag
     1321 cgcatccatc agcaacgttg ccagccagtg taaacgcgcc ggagcttgtt catgattaag
     1381 ggccgctagc agcgaatacc agtcgcccga tggcacgcta tatgccaacg cctgacacaa
     1441 tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgcgc caggcgaacc
     1501 ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac attgtcactt cgcgtgaaag
     1561 ccaggtcacg gcgtactgtt ccggcggcgg cgcaaggtaa tgtaaccgac aacgactacg
     1621 taatgttgcc agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg tttctgctgg
     1681 tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc
     1741 ggttacccag acaactttcg caccacctag gcgtgcgtgc tcattcagct tttcggtgac
     1801 ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt tcgggagcca gggtgtagta
     1861 atcgggatgc gtgccagcct gcatcaactg acatccacga cagtgaccgc aacttttgtg
     1921 gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag catcatcgcc
     1981 catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct gatagctggc
     2041 taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc caactccttc
     2101 acccagtggg tcacggtagt gcggattgca tccatcacgg cctccagcgg ctgggtggca
     2161 tcaatggtat gaatgctttt atcttgtgct gccagttcca gatagcgggc gcgggtgcga
     2221 ttaaagaaat cgaaagattc ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt
     2281 tttaagccaa cttccggggt aacatcgaga tagagcgtta agtcggggcg aaaatccccg
     2341 agaacagcat cacgcagtgt tgccagcata tgttggtcaa taccacgtcc gccgccctga
     2401 tacgcctgag tggagagatc gtggcgatcg ccaatcaccc aggtgccgtt agccagagct
     2461 ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag aacttcggct
     2521 ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct tcttaacttt
     2581 tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat acccagttgc
     2641 tcgagcgtct caaccaccac attacgcgcg gtagttttgc ctgcgccttc cagcccctca
     2701 atgacgatat acttactgcg catttttttc cttaagcact ttcagataat cctgcacaga
     2761 cttgttatga ctggcaagat tggtattaaa cgtgtgacca cctttaccat cggccacaaa
     2821 atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg cccccggcgt
     2881 agctatcgca cccggcggca aaccggtaat ggtataggtg ttatacgctg tcggcgtttc
     2941 caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa tcacggtcgg
     3001 gtcggtctgc aggcgcatac caatgcgtaa acggttgata aataccgagg caacctgatc
     3061 gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca ccagctggtt
     3121 tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga ccgctttcac
     3181 cattttcttg tgcgctcgct tgagtaacgc gacatcggtg gtattggcgg tatacatcca
     3241 ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc
     3301 tacggtggcg tacttatcgt cgctcagcgt gtgcttgata tacggagcct cacgcaattg
     3361 cttgaggtaa tcgctcagac gcatcccttc taccagtcgc agagggaact gtgcttcttt
     3421 accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt
     3481 cccggcttta aagtgagaaa gatccggttc gatacgcagc aaccattgga aaacccgtgg
     3541 gcgattgatg atcttatcgg cataaagctg ttcaccaagc gccagacgtc cggtccctgg
     3601 cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa gatggcgaac
     3661 cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca agataatcaa
     3721 aaacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata ttcatataac
     3781 gttgctgacg aaaaggagac atcgccacag gcacgtacgg gcatcactgg cattaacgca
     3841 ttacaaataa ccatctcatc tgcctgcaac gcctcttcca gagaggcttg cacttcgaca
     3901 agctgataag aggattgtgc cagtaaacgg atacagaatt gtcgcataat gccgttaaca
     3961 cctgcctgat ccagtcgcgg cgtataaact acattgccct tccgccagaa caaattagcc
     4021 gcacagcatt ccgtaaccca ccctgcgctg tcaaggacca gcgcctcatc agcgtttgtc
     4081 tgctcaagat gagagcggat caatacttgc tcaagccgat taagatgttt aatacctgca
     4141 agatgaggat tgcgccccag ccgcaccggg cttagcgcca acgtcatccc ctcattacgc
     4201 aaacggttgt aatgtgcagg ataagccgta acggagagaa tccgcgttgc cggtccgctg
     4261 ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt cagcacacca
     4321 ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgaggcca gaagtcacag
     4381 gaaatcatca accgctgaca agcatcctgt agtcgctgga tatgcgctga taacaaactg
     4441 actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg cgttgcccga
     4501 tcgcttactg ccagcgattc ctgcttatga ccgttaatta agaacatacc ggctccttat
     4561 cagagaattg ttagtgtggc agcatgttca atacggaaca agtcggaata aaagctaaga
     4621 aaaaaggccc gcaagcggac cttttataag ggtggaaaat gacaacttag atctttttaa
     4681 agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagagtg tattccattc
     4741 cgctaacctg acgcgcttcg tgcggtacga aatccagatc gcaaccttca tccgggttat
     4801 ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag tagatagatt
     4861 ctactgcacc cgccgcacct aacaggtgac cggtcataga tttcgtggag cttaccaaca
     4921 cacggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct ttatcgccag
     4981 ccggcgtaga agtaccgtgt gcgttaacgt agccaatctg actcgcttca atgcctgcat
     5041 cacgcaaagc atttgccatt gccagagctg cgcctgcgcc attttctggc ggtgacgtca
     5101 tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc
     5161 gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg cccagtacga
     5221 aaccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca ttgcgggtag
     5281 ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc
     5341 cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca tgaccaatgt
     5401 tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc aggccataca
     5461 tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag aatgggctga
     5521 tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt ccgaggccgc
     5581 caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc
     5641 cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag gcatccatct
     5701 tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact aagccagcaa
     5761 atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg
     5821 caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac atgcccagtc
     5881 cggtcacaac tacacgacgc ttagacacgt ttgtcctcca gggagggaaa aaatgattct
     5941 agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg cctggagatg ttcacttacg
     6001 cctggtggcc gttgatgtaa tcaatggcag cctgaacggt ggtgattttc tcagcttctt
     6061 cgtccggaat ctcagtatca aactcttctt ccagagccat taccagctca acggtgtcaa
     6121 gagaatccgc gcccaggtct tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa
     6181 cgcccagctg ttcgccgata attttcttaa cgcgttcttc gatagtgctc atactcttaa
     6241 atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt
     6301 tgcaactaaa tcccggcagg tcttaccacg attttacgtt attttgaggc cttacccccc
     6361 aataacgcaa atcattttgc actaattatt actcataacc acgcagactg cgcgcaacat
     6421 gagcattttg tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca tgcaaagttt
     6481 cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct
     6541 gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat acccgcacgc tggtcatcgc
     6601 tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc aacaacgttt acagtaatac
     6661 cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc gatcaagccc gctttcgcag
     6721 cagcgtagtt ggcctgaccg ccatttccca tggtaccaac cacagaaccg atagtgataa
     6781 tacgaccatg acgctttttc atcatagcgc gcattaccgc ttttgacaga cggaaaacag
     6841 atgaaaggtt ggtttcgata atatcgttcc actcttcatc tttcattcgc attaacaggt
     6901 tatcacgagt gataccggca ttattgacca ggatatccac ttcaccaaat tctgcgcgaa
     6961 ttttttccag aacagattcg atagatgccg ggtcggtcac attcaacatc agacctttgc
     7021 cgttagcacc taaataatca ctgatcgcct gagcgccatt ttcactggtc gcagtgccaa
     7081 taactttcgc gccacgggct gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg
     7141 caccggttac cagtgcgatt tttccttcaa aattcatgat tttcctcttt taaagctcga
     7201 gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc
     7261 gtttcgtcag gccagttagc actttgcccg ggccgacttc atagagatgt tctacgcctt
     7321 gcgctgccat gtactcaaca gacttcgtcc actgaaccgg gttatacaac tgacgtacca
     7381 gtgcgtcacg aatggcatca ccattggttt cgcatttcac atcaacgtta ttcacaacag
     7441 gaactgttgg tgcgttaaag gtgattttcg ctaattctac tgccagtttg tcggctgctg
     7501 gtttcatcag cgcacagtga gacggtacgc tcactggtaa cggcagcgcg cgttttgcgc
     7561 ccgcagcttt acaggcagcg ccagcacgct caaccgcctc tttatgaccg gcaataacca
     7621 cctgtcccgg agagttaaag tttaccggag aaacgacctg accttctgca gcttcttcac
     7681 acgctttcgc aatagacgca tcatccagac cgatgattgc cgccatagcg cccgtgcctt
     7741 ccggtacggc ttcttgcatg aacttgccgc gcatctcaac cagacgcacc gcatcagcga
     7801 aatcaatcac accagcgcaa accagcgcgg agtattcccc caggctgtga ccggccatca
     7861 ttgccggtgc tttaccgccc tgctgctgcc atacgcggta cagcgcaaca gatgcagtca
     7921 acagcgctgg ctgggtttgc caggttttat tcagttcttc agctggcccc tgctgggtca
     7981 gcgcccacag gtcgtagccc agcgccgcag aagcttcagc aaacgtttct tcgacaattg
     8041 gatagctcgc cgccatatca gccagcattc caacggtttg agaaccctgt ccagggaaca
     8101 caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc gcggagcccc
     8161 aggtgaatcc accgccaaag gcttcaagca gaaccaactg ccccggctta atgcgcccgt
     8221 cgcgtacagc ttcatccagc gcgcacggga cagaggccgc agaggtatta ccgtggcgat
     8281 ccagcgtcac cacgacattg tccatagaca taccgagttt tttcgccgtt gcactgataa
     8341 tacgcaggtt agcctgatgc ggaaccagcc agtccagttg agaacggtca agattattcg
     8401 ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg aagacttcgt
     8461 tgcccgccat cgtcagatga attgaattct ctggattcac gcggtcggcg tttggcagcg
     8521 tcagcaattc accataacta ccgtcggcat gcagatgggt ggagatgatt cccggctctt
     8581 cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa aataataata gtcccacgat
     8641 ctgttggatc gcaggtgcgc gccagtacat cggaaccgac gaccagagca tacttcaccg
     8701 ccccagattt cacgtattga tcggctacgc ttaatgcata ggtgaaacct gcgcaggctg
     8761 ctgcaacgtc aaatgccggg caacctttaa tgcccaacat gctttgaatc tgacaagctg
     8821 cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca atctggtctt
     8881 tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc aaagcccatg gttgaaacgg
     8941 tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt acgagtgaca atccactcgt
     9001 cagaggtgtc caccattttt tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc
     9061 tgccagtacc aataatcttc gtatacatgt acgctcagtc acttttcggt tatataccgt
     9121 cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc gcagagttcc gcttttgcca
     9181 ccgtccagca gctcaaaacc agctgggtat acagattcca ggcgagcggc aattcgctga
     9241 ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga
     9301 ttggctgcac catgactttt tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta
     9361 tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt
     9421 aataacagta gccaccacga ccgttttttc ccttcaccct gagatttcag cagagaaagg
     9481 aacatcctga caacaccttc catcgtcttt aacgtgacat ttcctgtaaa gccgtcacaa
     9541 accagcacat ctgtcttgcc agttaacaac tcattggctt caagatagcc gatataattg
     9601 atagaaggga ttgttttaag caccgctgag gcatcccgaa tactgtcgag accctttact
     9661 tcttcttcac caatattgag caacgccacg cgaggattgg gaatttccac cacctcttca
     9721 gccagaactg agcccataat ggcaaattgc accaacattg tgctgtcaca atcgacgttg
     9781 gcccctaaat cgaggaccac cgttttgccc ttttgctgat gtggtaatac cgtcaccagc
     9841 gccggacgct caatcccctc caggggcttg agtaataatt ttgccagccc catcagtgcc
     9901 ccggtattac cggcactgac acaggcttgc gctcgacctt ctttcaccag ctccagggcc
     9961 atacgcattg aactcccacg actggcgcgg atagcttgcg aaggccgggc atcactggcg
    10021 ataactgact gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc agctttagca
    10081 agtaatggcg tgatggcgtc gggattgccg actaaaagaa gagtgagttg cgaattagaa
    10141 ttcagtgcct gcaatgctgc aggcactgtc acggaagggc caaaatcccc tcccatgaca
    10201 tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt ctttccccag
    10261 ttgcctgggg aaaatcctca ctaagcttca tcacgcagac gcgtgattac ttagcgatga
    10321 ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt ttttcaccag
    10381 aagttttgtc tacagacagg ctggtgactg cggtcagcgc gtcatgggaa cgacgcatgc
    10441 cacgtttgga acgggttggt ttattctgtt gtacggccat ggaccttact cctcaattac
    10501 ggtttatccc cgatccaacg gggagcacca attacttacg ctttaagctg gctaatacgg
    10561 caaatgggtt tggcttttgc gcttcttcag gcagttcacc aaagaccatg tccgcttcgg
    10621 acacttcaca gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg atgatttcat
    10681 cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc ggttcatacg
    10741 cttccggcag tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa tacgttgtgt
    10801 agacctgatg agtaaacggc ttcccgcaac gctgacactc gagcgttacc gtcaccttcg
    10861 catcgccgtt taacactgcg agacgttggt tatcgatagc gaacgacatg gagcattcca
    10921 catcactgtc cacactgact acggattcgg cgacgcgctc aacctgatca ggggtataga
    10981 taccctggta atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc aggggtaatt
    11041 ttaccttttg catagggcgc gcatattaac tttgtaacgt catagagtca aagaaaaagg
    11101 cagcctgtgg ttgccttttg ccaataattc gcacacattg cgggttactg ctttattttc
    11161 gttcagtgct gataacgaac atcaacctgc aaaacgaagc gcatagttta aaatggctat
    11221 catcaatacg ctatatctga tggaaaaaaa tatgcctaaa cttattttag cctccacctc
    11281 gccatggcgt cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg cagcaccaga
    11341 ggtcgacgaa accccacgca gcgacgaatc accgcgacag ttggtgcttc gactggcaca
    11401 agaaaaagcg caatctctgg cgtcacgtta tccggatcat ttaattattg gttcagacca
    11461 ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta acggaagaaa atgcccgtct
    11521 gcaattacgc aaagccagcg gcaatatcgt caccttctat actggactgg cgttgtttaa
    11581 ttcggcgaat gggcatctac aaacagaagt ggagcctttt gacgtccatt tccgtcacct
    11641 gagcgaggcg gagattgata attacgtgcg taaggagcat cccctgcact gcgcgggtag
    11701 ctttaagagt gaaggatttg gcattacgct gtttgagcgt ttagagggac gtgaccctaa
    11761 cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg aagggaaaaa
    11821 cccgctgatg ggatagttgt ttgcgccgga tgcggttacg tcgcatccgg caatcggtgc
    11881 aggcggcctg atgcggcgta aacgccttat caggccaacc gcccattatc aggcttatat
    11941 gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg ctttaaacct tcatccatcg
    12001 gcgcttcgat acgcatcact tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt
    12061 gcaggaacag acgatttaat cccgtgcctg cttcagtgag ctgtctgtca aattcacggt
    12121 caccgtagcg atcgtcaaag gcaatcggat gacccgcata ctgtgtatgc acacggatct
    12181 gatgagtacg tccggttacc gggctacaac gcaccagggt ggcaaatgca tagcgttctt
    12241 ccactttaaa gcgtgtttcc gacggtttgc cttcctgact gacacgcacg atacgttcgc
    12301 cgctttgcag aatatttttc aataacggcg cttgaacgct cttcacatgc gactgccact
    12361 gaccgcgcac cagcgccagg taatcttttt gcattccttt ttcacgtaat tgctcatgca
    12421 gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac acctgaagtg tcccggtcga
    12481 gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcgataa
    12541 cgccgaagct taaaccactg ccgccatgta ccgccgtacc ggaaggttta ttcagcacca
    12601 ggatatgatc atcttcatat aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg
    12661 gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg tgggatacgc acctcatcac
    12721 ccgcttcgag tttatattca ggcttaatac gttttttgtt cacccgcact tcacctttac
    12781 gcaaaatacg gtaaatcata ctttttggta cgcctttcaa ttgggtacgc aaaaagttat
    12841 cgatacgttg ccccgcttcg tcagcggtga tagcaacaat ttttacggat ggagtctctg
    12901 ttttcatggt gggcgattct aattagccaa caggattcgc gccactcatt tttctatgct
    12961 tatatttagt tttgcacctt attacttcac tacgtgatca ctttattgat ggttattaaa
    13021 ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca
    13081 tcttgctata acaaggcttg cagtggaata atgaggccgt ttccgtgtcc atccttgtta
    13141 aaacaagaaa ttttacggaa taacccattt tgcccga
//
LOCUS       DAEVZA010000136        13096 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEVZA010000136 DAEVZA010000000
VERSION     DAEVZA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.136"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..908
                     /gene="glpK"
                     /locus_tag="LAF92_004053"
     CDS             <1..908
                     /gene="glpK"
                     /locus_tag="LAF92_004053"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBI9792976.1"
                     /translation="DKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPISGIAG
                     DQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGEVNYAL
                     EGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLGAPYWDPYA
                     RGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAVANNFL
                     MQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFRPGIET
                     TERNYRYAGWKKAVKRAMAWEEHDE"
     gene            1043..2053
                     /gene="glpX"
                     /locus_tag="LAF92_004054"
     CDS             1043..2053
                     /gene="glpX"
                     /locus_tag="LAF92_004054"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBI9792977.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            2150..2896
                     /gene="fpr"
                     /locus_tag="LAF92_004055"
     CDS             2150..2896
                     /gene="fpr"
                     /locus_tag="LAF92_004055"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBI9792978.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(2901..3329)
                     /gene="uspD"
                     /locus_tag="LAF92_004056"
     CDS             complement(2901..3329)
                     /gene="uspD"
                     /locus_tag="LAF92_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBI9792979.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(3356..3655)
                     /locus_tag="LAF92_004057"
     CDS             complement(3356..3655)
                     /locus_tag="LAF92_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBI9792980.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(3867..4307)
                     /locus_tag="LAF92_004058"
     CDS             complement(3867..4307)
                     /locus_tag="LAF92_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBI9792981.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            4408..5007
                     /locus_tag="LAF92_004059"
     CDS             4408..5007
                     /locus_tag="LAF92_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBI9792982.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            5115..5882
                     /gene="tpiA"
                     /locus_tag="LAF92_004060"
     CDS             5115..5882
                     /gene="tpiA"
                     /locus_tag="LAF92_004060"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBI9792983.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(5937..6692)
                     /gene="cdh"
                     /locus_tag="LAF92_004061"
     CDS             complement(5937..6692)
                     /gene="cdh"
                     /locus_tag="LAF92_004061"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBI9792984.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKCGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(6799..7788)
                     /gene="sbp"
                     /locus_tag="LAF92_004062"
     CDS             complement(6799..7788)
                     /gene="sbp"
                     /locus_tag="LAF92_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBI9792985.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(8108..9070)
                     /gene="pfkA"
                     /locus_tag="LAF92_004063"
     CDS             complement(8108..9070)
                     /gene="pfkA"
                     /locus_tag="LAF92_004063"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBI9792986.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(9251..10153)
                     /gene="fieF"
                     /locus_tag="LAF92_004064"
     CDS             complement(9251..10153)
                     /gene="fieF"
                     /locus_tag="LAF92_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBI9792987.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(10302..10802)
                     /gene="cpxP"
                     /locus_tag="LAF92_004065"
     CDS             complement(10302..10802)
                     /gene="cpxP"
                     /locus_tag="LAF92_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBI9792988.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            10952..11650
                     /gene="cpxR"
                     /locus_tag="LAF92_004066"
     CDS             10952..11650
                     /gene="cpxR"
                     /locus_tag="LAF92_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBI9792989.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            11647..13020
                     /gene="cpxA"
                     /locus_tag="LAF92_004067"
     CDS             11647..13020
                     /gene="cpxA"
                     /locus_tag="LAF92_004067"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBI9792990.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
BASE COUNT         3307 a         3388 c         3316 g         3085 t
ORIGIN      
        1 acgacaaaat gctggaagtg ctggatattc cgcgcgagat gctgccagaa gtgcgtcgtt
       61 cttccgaagt atacggtcag actaacattg gcggcaaagg cggcacgcgt attccaatct
      121 ccgggatcgc cggtgaccag caggccgcgc tgtttggtca gttgtgcgtg aaagaaggga
      181 tggcgaagaa cacctatggc actggctgct ttatgctgat gaacactggc gagaaagcgg
      241 tgaaatcaga aaacggcctg ctgaccacca tcgcctgcgg cccgactggc gaagtgaact
      301 atgcgttgga aggtgcggtg tttatggcag gcgcatccat tcagtggctg cgcgatgaga
      361 tgaagttgat taacgacgcc tacgattccg aatatttcgc caccaaagtg caaaacacca
      421 atggtgtgta tgtggttccg gcatttaccg ggctgggtgc gccgtactgg gacccgtatg
      481 cgcgcggggc gattttcggt ctgactcgtg gggtgaacgc taaccacatt atacgcgcga
      541 cgctggagtc tattgcttat cagacgcgtg acgtgctgga agcgatgcag gccgactctg
      601 gtatccgtct gcacgccctg cgcgtggatg gtggcgcagt agcaaacaat ttcctgatgc
      661 agttccagtc cgatattctc ggcacccgcg ttgagcgccc ggaagtgcgc gaagtcaccg
      721 cattgggtgc ggcctatctc gcaggcctgg cggttggctt ctggcagaac ctcgacgagc
      781 tgcaagagaa agcggtgatt gagcgcgagt tccgtccagg catcgaaacc actgagcgta
      841 attaccgtta cgcaggctgg aaaaaagcgg ttaaacgcgc gatggcgtgg gaagaacacg
      901 acgaataatg taaatgccga atgaagcgtt tatgccgcat ccggtagtcc cgaaacgtgc
      961 gggggcaacc ccgcacacat caataatccc tcccttcccc tgtgctacac ttcgcgccat
     1021 tccttactgc ttagagtttg ctatgagacg agaacttgcc atcgaatttt cccgcgtcac
     1081 cgaatcagcg gcgctggctg gctacaaatg gttaggacgc ggcgataaaa acaccgcgga
     1141 cggcgcggcg gtaaacgcca tgcgtattat gctcaaccag gtcaacattg acggcaccat
     1201 cgtcattggt gaaggtgaaa tcgacgaagc accgatgctc tacattggtg aaaaagtcgg
     1261 tactggtcgc ggcgacgcgg tagatattgc tgttgatccg attgaaggca cgcgcatgac
     1321 ggcgatgggc caggctaacg cgctggcggt gctggcagta ggcgataaag gctgcttcct
     1381 caatgcgccg gatatgtata tggagaagct gattgtcggg ccgggagcca aaggcaccat
     1441 tgatctgaac ctgccgctgg cggataacct gcgcaatgta gcggcggcgc tcggcaaacc
     1501 gttgagcgaa ctgacggtaa cgatactggc taaaccacgc cacgatgccg ttatcgctga
     1561 aatgcagcaa ctcggcgtac gcgtatttgc tattccggat ggcgacgttg cggcctcaat
     1621 tctcacctgt atgccagaca gcgaagttga cgtgctgtac ggtattggtg gcgcgccgga
     1681 aggcgtagtt tctgcggcgg tgatccgcgc attagatggc gacatgaacg gtcgtctgct
     1741 ggcgcgtcat gacgtcaaag gcgacaacga agagaatcgt cgcattggcg agcaggagct
     1801 ggcacgctgc aaagcgatgg gcatcgaagc cggtaaagta ttgcgcctgg gcgatatggc
     1861 gcgcagcgat aacgtcatct tctctgccac cggtattacc aaaggcgatc tgctggaagg
     1921 cattagccgc aaaggcaata tcgcgactac cgaaacgctg ctgatccgcg gcaagtcacg
     1981 caccattcgc cgcattcagt ccatccacta tctggatcgc aaagacccgg aaatgcaggt
     2041 gcacatcctc tgattgattt gatcgattga gccttccagt ccttcgggac tggaattttt
     2101 ttgttcggag aacgaagata aggcaagtca atcaaaacag gagaaaaaca tggctgattg
     2161 ggtaacaggc aaagtcacta aagtgcagaa ctggaccgac gccctgttta gtctcaccgt
     2221 tcacgccccc gtgcttccgt ttaccgccgg gcaattcacc aagcttggcc ttgaaatcga
     2281 cggcgaacgc gtccagcgcg cctactccta tgttaactcg cccgataatc ccgatctgga
     2341 gttttacctg gtcaccgtcc ccgatggcaa attaagccca cgattggcgg cactgaaacc
     2401 aggcgatgaa gtgcaggtgg ttagcgaagc agctggcttc tttgttctgg atgaagtacc
     2461 ggactgcgaa acgctatgga tgctggcaac cggtacagcg attggccctt atttatcgat
     2521 tctgcaacta ggcaaagatt tagatcgctt caaaaatctg gtcctggttc acgccgcacg
     2581 ttatgccgcc gacttaagct atttgccact gatgcaagaa ctggaaaaac gctacgaagg
     2641 gaaactgcgc attcagacgg tggtcagtcg ggaaacggca gcggggtcgc tcaccgggcg
     2701 gataccggcg ttaattgaaa gtggggaact ggaaagcgcg attggcctgc cgatgaataa
     2761 agaaaccagc catgtgatgc tgtgcggcaa tccacagatg gtgcgcgata cacaacagtt
     2821 gctgaaagag acccggcaga tgacgaaaca tttacgtcgc cgaccgggcc atatgacagc
     2881 ggagcattac tggtaagcgg ttacttatcg ataaacggca cgatgagcaa atccgcactc
     2941 atcttattga tcatcccgcg atatgccggc atcaaacggt tgataaatga gtgatgatga
     3001 ccacagacaa ggaggtcgca ctgctctttt tgcataattt ccagcagtgt ttccggcatt
     3061 tctccgcgtt caatacgcag ttttgtcttc ggccattgaa tatttttcgt cagtttatac
     3121 agcttgttat ccgacttatt cttcaacaat tgaagaatat cttctgttgc agggaagtag
     3181 atacccggat acaactcgct taagccatca tcaatatgaa ttaacgtcag gtgagcgtca
     3241 ttatgtctgg cgagctccag agctttattc accagtaagg catcttcttc attcccggaa
     3301 attgccacgc caatgtgttt ataagccatg tttaactcct tctaaagcaa ctccatcaag
     3361 ctaacgaacg cagtgataac tcaaaaataa tcatctcggc ctggcatgag aaagtgaagg
     3421 ccgcatcaag ctcaacttgc ccctccagat ccttaatctt gtaggccacg ctggcccctt
     3481 catctgcaga ggatgcagcg gcggcaagct ctttcagttt ggtcataaaa gactctgctt
     3541 cttcgcgggt ggcaaaaaag cgcgaaaact tactggtaca gttgtcgtta tcaatcaccg
     3601 taccgatatc aatcgcacat cctttagtac tgcatttatc tacgacatct ttcatagggc
     3661 acctctgtat attcgcctct ctgtcgatta cgacttaaga ataatgcgcc gttaagggaa
     3721 gaacaaacaa gtaatgatct tatggattac catttttttt gatattgctt aaggtgaaga
     3781 gttcagaagg attttccggg tattgtcatt tgcatcgtgg aaataacaat cccggaaaaa
     3841 aatagcagta agagatatat taaaggttaa ttacttgatt tattgtcggc tttatacttc
     3901 acatcctgag tatctttacc atatttattt tcgccttgtg tgccaacaaa cgcaccaaga
     3961 tcgataagca tcatcaccag aatcaacgtc gggacaaaac gccccaccgc ccattgccag
     4021 acacccggta aaatcgccca gttacccgcc agcagcatcc acgccacaat catcagaaat
     4081 gcccatgcgc cggaacgccc gcgatcatgc aagcgcttaa cagttactgc cgctgttggc
     4141 caaagcaagc acacaaggca aaacgccgcg gtctgaatat cgagtaaatt tttacccgcc
     4201 agtgagaaaa gcaccagcat gcctgcgaac cacaggccta tccaaatcca gaaatcacgg
     4261 cgtccaatac gccctttaaa tgagaataac cattgctgta tggtcatgta agttccttga
     4321 tggttgtctt ttccaggatt ctaccctttt gacaagggcg acagatatcg ttttaatcgg
     4381 agccagtcat aacaaaaggt actgtcaatg aagccagggt gtacgctgtt ttttctctta
     4441 tgttctgcat taaccgttac aacaacggcg catgcacaaa caccagatac ggcaacgacc
     4501 gcgccttatc tgctggctgg agcccctact ttcgatctct ccatcagcca gtttcgagaa
     4561 gactttaaca gccagaatcc tagcctgcca ctgaacgaat ttcgtgccat cgacagcagt
     4621 cccgacaaag ccaatctcac tcgtgctgca agtaaaatta acgagaactt gtatgcttct
     4681 acagcgctgg agcgcggtac cttaaaaatc aaaagcattc aaatgacctg gctacccatc
     4741 caggggccag agcaaaaagc agcgaaagcg aaagctcagg aatacatggc agcagtgatc
     4801 cgcacactca ccccattaat gaccaaaaca caaagccaga aaaaactgca gtcgctacta
     4861 acggcgggga aaaacaaacg ttattacacc gagacagaag gtgcactgcg ttatgttgtc
     4921 gcggacaacg gcgaaaaggg gctgaccttc gctgttgaac cgattaagct ggcgctatct
     4981 gaatcgcttg aaggtttgaa taaatgacaa aaagcaaagc ctttgtgccg atgaatctct
     5041 atactgtttc acagacctgc tgccctgcgg ggcggccatc ttcctttatt cgcttataag
     5101 cgtggagaat taaaatgcga catcctttag tgatgggtaa ctggaaactg aacggcagcc
     5161 gccacatggt tcacgagctg gtttctaacc tgcgtaaaga gctggcaggt gttgctggct
     5221 gtgcggttgc aatcgcacca ccggaaatgt acatcgatat ggcgaagcgc gaagctgaag
     5281 gcagccacat catgctgggt gcgcaaaacg tggacctgaa cctgtccggc gcattcaccg
     5341 gtgaaacttc tgccgctatg ctgaaagaca tcggcgctca gtacatcatc atcggccact
     5401 ctgaacgtcg tacttaccac aaagagtctg acgaactgat cgcgaaaaaa ttcgcggtgc
     5461 tgaaagagca gggcctgact ccggttctgt gcatcggtga aaccgaagct gaaaacgaag
     5521 cgggcaaaac tgaagaagtt tgcgcacgtc agatcgacgc ggtactgaaa actcagggtg
     5581 ctgcggcatt cgaaggtgcg gttatcgctt acgaacctgt atgggcaatc ggtactggca
     5641 aatctgcaac tccggctcag gcacaggctg ttcacaaatt catccgtgac cacatcgcta
     5701 aagttgacgc taacatcgct gaacaagtga tcattcagta cggcggctct gtaaacgcgt
     5761 ctaacgctgc agaactgttt gctcagccag atatcgacgg cgcgctggtt ggcggtgctt
     5821 ctctgaaagc tgacgctttc gcagtgatcg ttaaagctgc agaagcggct aaacaggctt
     5881 aagtctgaca ggtgccggat ttcatatccg gcacttactt tccttaactc ttcgccttaa
     5941 cgcaaaatct cacactgatg atcctgaatt tcctcggctg aagcacggtt aagcgtcagt
     6001 agatttcgtt gtgtcgccag caatacaaat gagttatcac tctgccgtac catcgccagc
     6061 ccgtagcttc ccatatgttc ccgcgcctca ggtacttctt ctgccagcat cataaatggg
     6121 ctgcgttgta ccagttcgct ttccgttacc cgacgcgcca ggtattcatg cccgcgcaaa
     6181 ccacctggca gtggcaacca gcggctgctg atgttcgcca gattgttatc cagttgttcg
     6241 cgcacatcag gacgaataca agagatatga atatgaaaat ggttttgcgt acgcccggtg
     6301 cgggagttga tcgccaaaga aaccgcgcga tcgggaaccg gctggccgca ttttttgctc
     6361 ataaaatcac gcgcctgcca ggccaaccaa aagaagttcg gcgttgaagg atcggtcaac
     6421 aaaggacttt cagtaccgtt aatacgatac gttggcatca acagatattg cagtggacca
     6481 ttaagatctt ttaaaaccac gtatccggca ttgggtttga cttccgcaca tggcgaagga
     6541 ttttgatttt gctgctgatt gggcaaacat tcctcaagga caatcttacg taatgtatcc
     6601 gactcttcac cggttaattt ccagtaacca ataccggcag ccacaacggc gataactatc
     6661 atcaccaaaa aaagaagacc cgcttttttc atcttttttt ccctgtacct caaagagatg
     6721 caagggtaac gcaaaatcgt gacaaataaa aaacccggtc gcgagaaacc accgggtttg
     6781 ataattatcc tggggaaatc agcgtttgct gatctgatcg aacgtaccgc cgttagcaaa
     6841 atgctctttt tgcgctttcg tccagccgcc gaactcttca tcaatggtga ataacttcag
     6901 ctttggaaac gcattttcgt actttttcgc cacctcagcg tcgcgcggac ggtagtagtt
     6961 tttcgcggca atttcctgac cttctggcga gtagagatat ttcaggtagg cttccgccac
     7021 ctctttggtg ccttttttct cgaccacttt atcgaccacc gacacggttg gctctgcgag
     7081 gatagactca ctcggcgtga cgatttcgaa tttatctttc cccagttcat tcgctgccag
     7141 cagagcttcg ttttcccagg cgatcagtac atcgccaatc ccgcgctcga caaaggtgtt
     7201 agtggagccg cgcgcgccag aatccagaac ttcgacgttt ttatacagtg cccgaacaaa
     7261 atcctgtgct tttgcctgat cgttgttgtt gtgatgcagc gcgtagcccc aggcagccag
     7321 gtagttccag cgtgcgccac cagagctttt cggattaggc gtgatcaccg aaacacccgg
     7381 tttaatcaga tcgttccagt catggatctg cttcggattg cccttacgca ccaggaaaac
     7441 aatggtggaa gtgtacggtg cggagttatc cggcagacgt ttgatccact ctttatcaat
     7501 ccgcccgcgt tccgcaattg cgtccacgtc ataggccaga gccagcgtga caacatcagc
     7561 ttcaataccg ttgattaccg acgtcgcttg tttacctgaa ccaccgtgtg actgacgaat
     7621 caccacgtta tcaccagttt gctgtttcca gtgggcgctg aatgccttgt tgtactgttc
     7681 gtacaattcg cgcgttggat catatgaaac gttaagaagc tgaatatcct ttgccataac
     7741 gctggttgcc gccagcaaaa atgttaaccc tacgccccac ttgttcatcg cccgactctc
     7801 ttatgttgtg ttgtgatgag caaagcgtgc cagaaggtta accaaacatt aaagaataaa
     7861 aaaagattgg ctataacttg cgggtatatg ttgaaggatt aaaaaggcgg aagaaatccg
     7921 cctcatattg ctgacaaagt gcgctttgtc catgccggat gcggcgcgaa cgccttatcc
     7981 ggcctacaaa agtttgcaaa ttcaataaat tgcagaatta atgtaggcct gataagcgaa
     8041 gcgcatcagg catttttgct tctgtcatcg gtttcaggct aaatgaatct gcctttttcc
     8101 gaaatcatta atacagtttt ttcgcgcagt ccagccagtc gcctttgaac ggacgcttca
     8161 tgttttcgat agcgtcgatg atgtcgtggt gaaccaactg ttcgttctgg atacctacgc
     8221 aacgaccgcc gtaacctgcc agcagcagat cgatagcgta agcgcccata cgggaagcca
     8281 gaatacggtc gtaaggcacc ggagaaccac cgcgctggat gtggcccagc acagttgcgc
     8341 gggtttcacg accggtttct ttctcgatga aatgcgccag ttcgtcaaca tcacacatat
     8401 gttcggtaat cgccacgatc gcgtgttttt tacctttcgc gatacccgct ttgatttcgt
     8461 ttaccaggtc ttcacggctg aattcaactt ccggaaccac aacgaattca cagccaccgg
     8521 caatggccgc agccaacgtc agatcgccac aataacggcc catcacttcc accacggaaa
     8581 tacgctggtg agaagaagag gtgtcacgca gacggtcgat cgcttctaca acggtgctca
     8641 gcgcagtgaa gaaaccgata gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg
     8701 gcagaccgat gcacgggaag cccatttcgg tcagacgcat tgcacccatg taggaaccgt
     8761 caccgccgat aaccaccagc gcgtcgatcc cacgtttttt caggttttcg atagccacgg
     8821 cgcggatgtt ctcgtcgcgg aattccggga agcgcgcaga accgaggaac gtaccgccac
     8881 ggttgatcat gtcagaaacg ctgtaacggt ctagctgtac catacggtct tcatacagac
     8941 ccagatagcc gtcataaatg cccattactt ccagaccttc tgtcagcgca gaacgaacaa
     9001 ccccgcgaat tgcggcgttc atgcctggcg catcaccgcc gcttgtcaac acaccgattt
     9061 tcttaatcat gactacctct gaactttgga atgcaaaata aaatctgttg ccggaagtct
     9121 tcttgcacat cgaagtgatc caacgaatgt gcaaatagta taacaatcac ttcctgctga
     9181 attgattcag gtcaggccaa atggcggtat tttatacaca aaatgcgggt ctggctctct
     9241 tttatactga ttatgaaagc atagaccgtt taccctccct gggtacgacg gaacaggggt
     9301 cctgatggat aattacatcc gatcccggaa aacgccgtaa aatagcctgc tctacctgat
     9361 ccgccaccat atgtgcctga accaaaggca gagagtcttc catttccaaa tgaatctgaa
     9421 taaagcgggt cggccctgac tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc
     9481 aggaagtcac gatatcaata atttcttgcc gttcctcatc aggcaatgcg cgatccagta
     9541 atgactgtac cgcctcatat cccatgcgta acgcgctata taaaatatag atgccgattc
     9601 ccaatgcaaa cagagcatcg gcgcgatgcc agccgtacca ggacaacccc agcgccagca
     9661 gaattgcgcc gttcatcata acatcagact ggtaatgtag catatcagcc cgcaccgcct
     9721 ggctttgcgt ccggcgcacc acccaacgct gaaacgagac aaggataatc gtacaaatta
     9781 gcgccacaat tgtcacgata accccgacgc ctggatctgt catcggtgtt ggagatatca
     9841 gatgttgaat acccgtcaaa aacaggaata gtgccgaacc ggagataaac atactttgcg
     9901 ccagcgccgc gagggactct gctttaccgt gaccaaacga gtgattatcg tcggcaggtt
     9961 gcagggaata tcgcaccacc agtaaattcg tcaacgacgc gccgatatcc accagcgaat
    10021 ccaccagcgc ggcgagaata ctcaccgacc cggtatacca ccatgcaaaa attttaatca
    10081 gcaatagcaa cgaagccatc gccgtcgcag caatcgccgc ccgactgacc agccgtccat
    10141 aagattgatt cataaatact cccgctatca actgacgcta gtataacgga agcaaatcat
    10201 ctgcaatgca ttaagcagca ggcaaattga ggataaaaaa aacccccaca tcatgtgggg
    10261 gaagacaggg atggtgtcta tggcaaggaa aacagggttt actactggga acgtgagttg
    10321 ctactactca atagcttcaa cgatgaactt ttttgccatt gcgtcacgtc acgcaactgc
    10381 tccattcgtt gttgatgttt ctcgtttaaa accgcttgct gctccggcgt taacaggcga
    10441 tacatttggt tgcggacttt tgccatctca acctgacgag caatttgctc attcgccatt
    10501 ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt ctgcggtgac aaggcgatgc
    10561 attgtctcca gttcgctaac attaacagga ggctgttcgt gccgggcctg ttgcataaga
    10621 tctcgcatct gctgacgctg atgttcggtt aaacttatgc cgtcgaacat atggctctgc
    10681 gtactgcgct gcgtaagttc ttcacccgga tgccagttat cgcctgaacc gacttcagca
    10741 gcgtggctta atgaactgac tgccagcgtt gaggccatga cggcagcggt aactatgcgc
    10801 atcatttgct cccaaaatct ttctgtcgcg attcaacgat agagagttta cgattcaggc
    10861 tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca tggattagca acgtctgatg
    10921 acgtaatttc tgcctcggag gtatttaaac aatgaataaa atcctgttag ttgatgatga
    10981 ccgagagctg acttccctat taaaggagct gctcgagatg gaaggcttca acgtgattgt
    11041 tgcccacgat ggggaacagg cgcttgatct tctggacgac agcattgatt tacttttgct
    11101 tgacgtaatg atgccgaaga aaaatggtat cgacacatta aaagcacttc gccagacaca
    11161 ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa cttgatcgcg ttctcggcct
    11221 tgagctgggc gcagatgact atctcccgaa accgtttaat gatcgtgagc tggtggcacg
    11281 tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa cagcaaaaca acgacaacgg
    11341 ttcaccgaca ctggaagttg atgccttagt gctgaatcca ggccgtcagg aagccagctt
    11401 cgacgggcaa acgctggagt taaccggtac tgagtttacc ctgctctatt tgctggcaca
    11461 gcatctgggt caggtggttt cccgtgaaca tttaagccag gaagtgttgg gcaaacgcct
    11521 gacgcctttc gaccgcgcta ttgatatgca catttccaac ctgcgtcgta aactgccgga
    11581 tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt cgcggctatc tgatggtttc
    11641 tgcttcatga taggcagctt aaccgcgcgc atcttcgcca tcttctggct gacgctggcg
    11701 ctggtgttga tgttggtttt gatgttaccc aagctcgatt cacgccagat gaccgagctt
    11761 ctggatagcg aacagcgtca ggggctgatg attgagcagc atgtcgaagc ggagctggcg
    11821 aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt tccgggcgat tgataagtgg
    11881 gcaccgccag gacagcgttt gttattggtg accaccgaag gccgcgtgat cggcgctgaa
    11941 cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg ccgataacgc cgatcatccg
    12001 cagaagaaaa agtatggccg cgtggaactg gtcggtccgt tctccgtgcg tgatggcgaa
    12061 gataattacc aactttatct gattcgtccg gccagcagtt ctcaatccga tttcattaac
    12121 ttactgtttg accgcccgct attactgctg attgtcacca tgttggtcag tacgccgctg
    12181 ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta agctgaaaaa cgctgccgat
    12241 gaagttgccc agggaaactt acgccagcac ccggaactgg aagcggggcc acaggaattc
    12301 cttgccgcag gtgccagttt taaccagatg gtcaccgcgc tggagcgcat gatgacctct
    12361 cagcagcgtc tgctttctga tatctctcac gagctgcgca ccccgctgac gcgtctgcaa
    12421 ctgggtacgg cgttactgcg ccgtcgtagt ggtgaaagca aggaactgga gcgtattgaa
    12481 accgaagcgc aacgtctgga cagcatgatc aacgatctgt tggtgatgtc ccgtaatcag
    12541 caaaaaaacg cgctggttag cgaaaccatc aaagccaacc agttgtggag tgaagtgctg
    12601 gataacgcgg cgttcgaagc cgagcagatg ggcaagtcgt tgacggttaa cttcccgcct
    12661 gggccgtggc cgctgtacgg caatccgaac gcactggaaa gtgcgctgga aaacattgtt
    12721 cgtaatgctc tgcgttattc ccatacgaag attgaagtgg gctttgcggt agataaagac
    12781 ggtatcacca ttacggtgga cgacgatggt cctggcgtta gcccggaaga tcgcgaacag
    12841 attttccgtc cgttctatcg gaccgatgaa gcacgcgatc gtgaatctgg cggtacagga
    12901 ttggggctgg cgattgttga aaccgccatt cagcagcatc gtggctgggt gaaggcagaa
    12961 gacagcccgc tgggcggttt acggctggtg atttggttgc cgctgtataa gcggagttaa
    13021 actccgcatt tgtaggcagg ataaggcgtt tacgccgcat ccggcatttg agcaggatgc
    13081 ctgatgcgac gctgat
//
LOCUS       DAEVZA010000137        12970 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEVZA010000137 DAEVZA010000000
VERSION     DAEVZA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.137"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..823)
                     /locus_tag="LAF92_004068"
     CDS             complement(<1..823)
                     /locus_tag="LAF92_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBI9792991.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASEL"
     gene            complement(820..2217)
                     /locus_tag="LAF92_004069"
     CDS             complement(820..2217)
                     /locus_tag="LAF92_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBI9792992.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2372..3370
                     /locus_tag="LAF92_004070"
     CDS             2372..3370
                     /locus_tag="LAF92_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI9792993.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3481..4386)
                     /gene="ompG"
                     /locus_tag="LAF92_004071"
     CDS             complement(3481..4386)
                     /gene="ompG"
                     /locus_tag="LAF92_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBI9792994.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(4431..5513)
                     /locus_tag="LAF92_004072"
     CDS             complement(4431..5513)
                     /locus_tag="LAF92_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI9792995.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFVLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIALR"
     gene            complement(5527..6186)
                     /gene="pgmB"
                     /locus_tag="LAF92_004073"
     CDS             complement(5527..6186)
                     /gene="pgmB"
                     /locus_tag="LAF92_004073"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBI9792996.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            complement(6183..8450)
                     /gene="ycjT"
                     /locus_tag="LAF92_004074"
     CDS             complement(6183..8450)
                     /gene="ycjT"
                     /locus_tag="LAF92_004074"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBI9792997.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELGLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(8447..9502)
                     /locus_tag="LAF92_004075"
     CDS             complement(8447..9502)
                     /locus_tag="LAF92_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI9792998.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLRDQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(9512..10300)
                     /locus_tag="LAF92_004076"
     CDS             complement(9512..10300)
                     /locus_tag="LAF92_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBI9792999.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(10318..11370)
                     /locus_tag="LAF92_004077"
     CDS             complement(10318..11370)
                     /locus_tag="LAF92_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBI9793000.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMEVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(11401..12243)
                     /locus_tag="LAF92_004078"
     CDS             complement(11401..12243)
                     /locus_tag="LAF92_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBI9793001.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(12230..>12970)
                     /locus_tag="LAF92_004079"
     CDS             complement(12230..>12970)
                     /locus_tag="LAF92_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBI9793002.1"
                     /translation="PNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLG
                     LAVAMFFNREFRLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLY
                     EQAPLWFDNPGSSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQR
                     FRIVTLPAIMPVLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKA
                     AAISVVLFIIIFAVILLTRKRVNLNGNK"
BASE COUNT         3090 a         3638 c         3225 g         3017 t
ORIGIN      
        1 ccagttcgct ggctccggca aaagcgatat tcagcaatac cagcttaaac agacgcaaac
       61 ggctgtaata ccctagttca atgccataca gcgtggcgat gcgattaatt aaacgcagat
      121 tgcgccaggc gataaacgcc atatcgacca acgccagcgg gctgaccgca atcatcaacg
      181 ttgattccgc cgccgaacgg ctgatttcgc gccgcgcctg ggcatctaaa actggctgga
      241 ccaaatgcgc atacaaactg accacttcac ggtcgttttg cgtttcatgg attgaggcat
      301 accagcgttg cagcgctgga tgagactgat caatacccgc ctgctgcgcc agtttttcgc
      361 aaaatgcgcg gcctttgccc gtgccgtggc tatgcaataa atcacgcgct tcgtcgcgtt
      421 catgggcgcg ctgtcgcaag cgccataagc gccgccactc tgttaccaca gaacctacgc
      481 cagcgccgat aatcaatgcc ccagcggcac atccacccag cgccacccag tcctgggttt
      541 gccaggcatt cattgtccac tgtacaccct gcccgacaac gcttgcgcca aacagagcca
      601 gcccgcccat caccattttg cgccacaggc tgcgtttcgg acgtaacgct gcgtccatta
      661 ccgcttcgac ttgcccctct tcttcctgcg cttcgtcgag cgtggccggg gcaaaatttt
      721 gcgcctgatt ttcgtcaaag gtctgctgcg ccctgaattt aggattctga tcgacctcca
      781 gaggaccgtc gaaatcaata cgtggtttta acggttcggt catcgcaatt tatctcctat
      841 taaaaattcc agcgcagcat caagacgaat atgcggtagt ggtttgtcga catccatcac
      901 ctgcggacga aatgcctcaa actggaagcc ttgcttatcc cagaacgcct gaccaggcaa
      961 acgtgcggga acttcgccag gataaacagt gagcggtgcg ccatcgctaa gtcgattacc
     1021 acgcagcgcc gggattttct caccgttaac atcaataatg ccgctggtgg tcgcctgaac
     1081 tgacgccagc cccaggcagt ccatgctgat cccttcgaac gccgcatttt gccaggcatc
     1141 ctgaatcagt tgttgcagta atgaaaccat attagcgtgc tgatcgatgg tcacatggtc
     1201 cgctttagtg gcagcaaaca atagcttatc gataaccggc gaaaacaaac gccggaacag
     1261 ggtacgctgc ccgtagtgaa aactttgcat cagctgcgtc agtgccagac gcatatcatt
     1321 aaatgcctgt ggcccactgt tgagaggttg caggcaatcc accagcacaa tctggcggtc
     1381 aaagcgcaga aaatgattct tatagaaccc cttcaccacc ttctcgcagt aataattaaa
     1441 gcgctcgcgc agcattccgg cattggtatg cttatcggcc tgcgccagtt tggactcgcc
     1501 ccaggtatcg acatccggcc acgggaagaa ttgcagcgcg ggcgcacctg ccatatctcc
     1561 cggcaagaca aagcgcccag gctgaataaa gtgcagcccc tgctgtttac agtggtggag
     1621 ataatcggtc cacgcggcgg caatgtctgc cagccggttt tcgtcggcag gcgctagcgg
     1681 gtccagccct tcgcacatca ttcgccattt gaccgaccat tcgccgcgct gaccattgag
     1741 taagcccgtc atctggcgtg accagcttaa atagtcctgc gccagcatcg gcaggtcgag
     1801 caaccattcg ccgggataat ccacaatctc cagatacagc gtggaggtgt ccttgaagtg
     1861 gcgtagcagc gaatcgttcg atttaaaacg tagcgccagg cggatttcac tgacgccgcg
     1921 cgttggcgtc ggccaggcgg gaggatcgcc atataactgc gccagtcctt catcatatgt
     1981 gaagcgcgga atgccaaagt cacgctgagg aatgcgtttt acgcccagca ggcgctcttc
     2041 acgcaccgcg cttaacagcg gcaagcgtgc tccggcgtga atattgagca actgattgac
     2101 catcgcagtg ataaacgctg ttttgccgct gcggcttagt ccggttacgg cgaggcgcag
     2161 atgtctgtcg acaccccgat tcaccagcgc attaagttca tttttaagtc gcttcattgc
     2221 tgtcctgtgc tgctctgaag accagaaaaa aatcattcgt aaggatatga atgtgggggc
     2281 caggccattg attatcaact gcccgtgttg tcatcgacgg gcgaactcca taagatgtgc
     2341 agcattcacg ttcggacgga gttcctgacc tatgtcccct actatttatg atattgccag
     2401 ggttgcaggc gtatcaaaat ccaccgtatc acgcgtgctg aataagcaaa ctaatatctc
     2461 cccggaagcg cgcgaaaaag tgttacgggc cattgaagaa ttacagtatc agccaaacaa
     2521 gctggcccgc gcgctgacct cttcgggttt tgatgccatt atggtgattt ctacccgttc
     2581 gaccaaaact acggcgggta atccgttttt ctctgaagtt ttgcatgcca tcaccgccaa
     2641 agctgaagag gaaggtttcg acgtgatatt gcagacgtcg cacaatccgg cagaagactt
     2701 acaaaaatgt gaaagcaaaa ttaagcagaa aatgattaaa ggcattatta tgctaagttc
     2761 gccagcggat gagtcatttt ttgcccaact tgataaatac gatattcctg tcgtggtgat
     2821 tggcaaagtt gaaggtcaat atgcccatgt ttattctgtc gataccgata attttggcga
     2881 cagcattgcg ttgaccgatg cattaattga aagtgggcat caaaatattg cctgcctgca
     2941 tgctccgctt gatgtccatg tttcagtgga tcgggtaaat ggttataagc agagcctggc
     3001 tgcgcataat attgcagtgc gtgatgaatg gattgttgac ggcggttata cccatgaaac
     3061 agcactgcaa gccgcgcggg aattattaag ccagtcaccg ttgcctgagg cagtgtttgc
     3121 tactgacagc ctgaaattaa tgagtattta tcgtgcggca gcagagaaaa atattgctat
     3181 tccgcagcag ttagcggtgg tgggttatag taatgaaacg ctgtcgttta ttttaacgcc
     3241 tgcgccaggc ggtattgata tcccaacaca ggagttaggt caacaaagct gtgagttatt
     3301 attccgctta atttctggaa aaccgtcacc acagaatatt accgttgcca cgcatatgac
     3361 gctgaaataa ataccagcta ccttgcctga atgccggatg cgatgctagc gcatcttatc
     3421 cggcctacat ttatagttcg gataaagtac tcgtgccgca tccggcaatt tagcccatta
     3481 tcagaacgag taatttacgc cgacacctgc ataatggaat ttatcgctgt cgccttcgtc
     3541 gtgatcctgc cactcaaacg cgtattccag cgaaacggaa agcccgttct ggaaatcata
     3601 accgtaaaat aaacctacac ggttaaaatc atggccttca cgttcaatat catcctgcca
     3661 gtcccagtta ctccagcgat ccagcccaat gcgcgtatac ggcgtcaccg agtggttgcc
     3721 gagcgtcagc ggcaaatagg cgcgaatttc ttgcgtagaa aactcaccgt tattgcggct
     3781 gtcgtccata ttaaagccgc gctcgagata atagttcact cgcaaggcaa ccgtttcgtt
     3841 gaaggtatat tgcaggcctg tttccgtttc gactcgggta tcagcgtaac cggtcgtgtt
     3901 cagatcgttg gcaaatttat acatcgataa ccaaccgttg aaacgtaaat cgtccgtcag
     3961 tttcacatcc cagtctggcg cgattttcca gcgctgcata ttcgccgtgt ctttacccgg
     4021 ttcatcaacg tagtgataac cataattacg gaaaccaccg gtcaggccga aactgaaatc
     4081 atcgttttcg aggaactgat aatgcacctc cagctccggg cgatcaaacc acgttccacg
     4141 tttacccgcg ctataatcta ccggcccttc ctgataatag gccagagcaa ttctccacgg
     4201 cccgttggcg gcattaaaat agactgaagg ctccgccagc ccatccatat cttcgccata
     4261 accctcgacg ttttctattt cgtacatcgc gccgatatta aagtgccagt cgttcttttc
     4321 ctcggcctgt gcgcaggcca ttcccgcaca catcaccagt gcggtacagg gtaataactt
     4381 tttcattatt gttccttgta ttatcctggg ggaatgcctt ccccctgtat ttagcgaagt
     4441 gctatttccg tttctgcatc aaagaaatga catttcgtca tatcaaaatg aatagtgata
     4501 ttttctcctg catgataatc atttaacgca ccagcacgga ccactaactc gtgcccccca
     4561 accgtggtgt agagcacaaa ttccgcaccg gttaattctg ccacgctaat tttggcggaa
     4621 atgttatttt cctcttgcgc gtccggatga atatcttccg gacgtattcc catcacgatg
     4681 ggcttatgca aacttccctg tgttttcaga accgctaatt tctcttcggg aatggttaat
     4741 ttaagcgttt ccgtaacgaa tttatcgcca tcgatcgtgc cacgaataaa attcatcgcc
     4801 ggtgatccaa taaatccggc aacaaacata ttcgctggtt ggttataaac cgttttcggc
     4861 gcaccaactt gctgaacaat cccatccttc ataatcacaa tccgcgtcgc catggtcatc
     4921 gcttcggtct gatcgtgggt cacgtagatc atggtggtat tcagcttctg gtgtagcttg
     4981 ctgatctctg cgcgcatttg cacgcgcagc ttggcatcaa ggttagagag cggttcatcc
     5041 attaaaaaca cgcccgcttc gcgcacaatc gcccgcccga gcgccacacg ctggcgttgc
     5101 ccgccggaaa gcgcgcccgg cttgcgtttc aggtactcac gcaggccgag aatttgcgcc
     5161 gcccagttca cccgctcatc aatcacctct ttggcgattt tttgcatctt caggccaaac
     5221 gccatgttgt cgtaaaccgt catatgcgga tacaacgcgt agttctggaa caccatcgct
     5281 atattgcgtg ctttggctgg aacgtcattc attcgtttgc cgtcgatcaa cagatcgccg
     5341 ccgctgatct cctcaagccc ggcaatcatg cgcagggtgg tcgacttacc gcagcccgac
     5401 gggccgacaa acacgatgaa ctctttatcg gcaatttcca ggttgaagtc cttcaccaca
     5461 tgcacctggt tatcgtagat tttttgaata tgttgtaacg aaagctgagc catgttgatt
     5521 cctttgctat acgttttgcc agaaggccga taaccgcggc caggtgagtg attccgttga
     5581 aggcaacagt aattgcgccc cggttaagcc cgcgccgatc cccaccgagc gcataccgct
     5641 ggcgttaatg gcgtcaatgc ccgcctgcgc atcttcaatg ccgatacatg cctgcggcgg
     5701 cacgcccagc ccggcacagg cggcgagaaa gatttccggg tccggtttcg agtttttaag
     5761 ttgggaagca tccgcgcaga aggtgaaaaa ctcgcgcagc tccagcgccg ctaaaatcgt
     5821 cggcgcattc agggagacag aagccagccc aaccgggatc tgctgtgcac ggagatctgc
     5881 cagcaaagag cgaatgccgg gtagaacagc gctgaccgtc aactcgcgca gtgagtggac
     5941 atagagcaga tttttgcgat acgccagttg cgccctctcc tgcgagttaa agtcgccctc
     6001 tttgcccccg tgttgcagaa tgcgccgcag agactcatcg cggctgatcc cttttaggga
     6061 ttcgttaaac tgcgcatcaa tgctgatgcc aatttcagcg gcaatctgct gccacgcctg
     6121 gaaatgcaga tgcgcggtat cggtgattac accatccaga tcgaaaatta ccccttgcag
     6181 tttcattcat cctcctgatg tgtggtagcg gtcccattga agggcaaaat aaaatcaccc
     6241 aaacagaaaa cagattcttc agccacggat ataagctgac cgttcaaacg cagtgaaacg
     6301 ggcgcagaag ttcgaatcgc aatacgctgc gcgtcaagag tgacctgtaa ttcgcagccc
     6361 tgccagaaca gagggaaaga caactgttgc cactgctcag gtaacgccgg attgagatgc
     6421 aattcaccgt cacgcacgct caccccggca aaaccctgaa tcgcccccag ccagatagcg
     6481 ccagttgcgg cagcatggat accatcatca caactatgcg gatcagcacc aagatcgatt
     6541 tcagtcccct cgcgccagaa ctgataactt tgggtcagca ggccacagcg tgcggcaaca
     6601 atgccgtgga ttgctttact taatgacgag tcgtgaatag tgcgcggttc ataaaattgc
     6661 agattggcaa gacacgatgc cgctgagaac tgctccggca gcatgtaatt gagcatcacc
     6721 acatcagctt gtttgaggat ttgcatctcg ttcacttctg cgcgtgaata atccagcaga
     6781 atggtttgct tccccgccgc cgctttgtat ttcgccagat taatcgccgg cttagccata
     6841 aacgaatcat cctgcggcaa aacgccgtcg ggctgaattt ctggcagccc cagttcttta
     6901 aggaacattt cggcgcgatg gataaacgca tcgtcgctac agccaaactg gcgggcaata
     6961 ctcagcgcct gctggacgtt gtaatacgcc atatagctgg tgaaggcgtt attattgaca
     7021 tgttcggtat attcgtcagg cccaataaca tcatgaattt ccagacggtc gttaaccctc
     7081 accgcgcggc taatccagaa ctttgcagtt tccagaagta gcgccatgcc ttcatgcgca
     7141 atgaaacttt catcccccgt ggtctgccag tattgaataa ccgcccaggc gatatcggcc
     7201 accagatgat gttccgcctg cgccgaggcc actttttgcc gcagcccggt gcgaatgtta
     7261 atggcggcaa attccggcgt ctcttcttcg ccgctgcgcg cgctttccca cggaaatagc
     7321 gcgccctgcc agccgttgcg tcgcgctttc tcctgcgcgc ctggcaagtt gtgccagcga
     7381 taacgcagta aacttcgggc aaccgtcgga tcgctaaaca gatgaaacgg taacaaaaat
     7441 acttctgtat cccagaaaac gtggcctttg tagccttcgc cggttaagcc ttttgccgca
     7501 atgctgctgc gctcgtcgtg ggcaggcgtc atgatgcgca gatgataaag cgcataatct
     7561 aacgcttgct gatcgtgcgc ttcgccgcca tttaccgtga tacgacgttt ctgccaccat
     7621 tgacgccagt tttctgttga tactgcaaga agttggtcgt aactctgctg cgcgcacatt
     7681 tcaagctggc gaagcgacgc gctgccccac tcgtctaaaa cggcttgcct gtcatcccgc
     7741 cagtcgatcc acaccagttt ttgcaacgtc actgtctcgc ctgcatgaag ctgcgcactg
     7801 gtatgttgct gcaaacggcg ctctttggcg gtatagcatt gctgcacatc accgctcacc
     7861 atacagcaac agctgatggc cacatcactg cgtccatcct gggtggtgta gatcccctgc
     7921 atcagatgct gaccaaacac ccgcacctgg gtttcgtcga gatgttggcg accgtggttg
     7981 gtttgcgtgg cgtcgatacc tgttgaaatc agcactgacg cgtcggcgtc cagtggcgta
     8041 atagtgattt ccagcacaat gagcggcagt tggtctgccg aaacaaagcg acggctggcg
     8101 atggtgtaac ctgtgccgtt gctggtacgc cagacaacgc tacggcgtaa ttctccactg
     8161 gcaaagtcaa gttcacgctg ccaggcttcg cgggataacg agaaaacctc accatttatg
     8221 gcaatctcca tccccacgac atcaggcagg ttcaccagtt cgttgatttc accttttccc
     8281 gcccgatgat acagccccgc cagatacatc cctcgcgtct ggcgcgtgta atcttcttca
     8341 tggctggcgc gaagcccaag atagccgttc ccctgcgcca tcagcgatgc atacttgttc
     8401 agggtatgct ggctaaaatg gggttctgat aacgttactg gcctggtcat aattccacac
     8461 gcgtccctgt ttctgcggat tgatataacg ccgccaccag ttgctggatg atgtacccct
     8521 gctcggcgtc ggctatcatc acgggcttgc cctgtacgtg attgataaag gcttccatgc
     8581 tgcgcaaatg gcggttgtcg tctgccattt cccgttgcat cagcgtcatt aattcaccgt
     8641 tgttatcggt gtagatatgt gctggaaaca gcgtcgcacc ggctttatca ccacaaaagc
     8701 tgacgttcat aatcgactgt tcgcggatgt tgagtgcaaa tgacgtttcc agccacagaa
     8761 tgccgccgtt atgaaattca atggtgccaa acagcgaatc ttcgacgcta taagttgccg
     8821 gatcccactc gccaaattga ccacagctct tttgcgtgcc gatcttttga aagctatgcg
     8881 cattcacgct tttcaccgcc ggaaagccca gcacatacat cgcagcatcc agcatatgaa
     8941 tgccgatgtc gatcagcggg ccaccaccct gcagttcttt attggtaaag acgccccagc
     9001 caggaacgcc gcagcgacgc agggcgcggg cggtggtaac gtaaatctct cccaaaacgc
     9061 cgttggtcac ctggtcacgc agctgttgcg tatcgagcgc aaaacgatgg tgaaagtcgt
     9121 aggccagcac cttgcccagt ttgcgcgcgg tatcgcacat ttcccgcgcc tgttctggcg
     9181 tcatggcggg cggtttttcg cacatcacat ggcagcccgc ttccagtgcc atcagggtat
     9241 gttcgtaatg aaaacggtta ggtgagcaga cgctaaccac atcaggtttc accgccagca
     9301 gcatggcctg cggatcgtcc cacacggatg cattcccgta tttttctgcc agcgcctgcg
     9361 cctgggaaag gcggctgtca cagacagcca ccagttccag atcgttgcgg gtgcagtagt
     9421 acgaagcatg aaccttatcc gccacctggc ctgcgcctat tatcgcgacc cgcagcggag
     9481 agcttgtcat tgcacttttc acaaagacct cttagcaggt acgcaaccag gccaacgaat
     9541 cacggtacgc ctgggcagga tcttccgccc ggatacgccc ttcgtacact acatagccct
     9601 gatagttatc cgcgcgcagc tgttcaaaca gcgcgtggaa atccagggtg ccgctgcccg
     9661 gctggtagcg atggttatcc gcaatatgca catgaccgag caggtcgcgg ttgtcatgca
     9721 gcgcctgcgc caggttatct tcttcgatat tcatgtgata gaaatcgccg ataatctgta
     9781 catgtttaag atcgttttcg acgatgtaac ggcgggcatc ggcgagggtg ttgatcatat
     9841 gatcctgata gcggtttaac ggttcgagat acaccacggt tccggtacgc gcggcgacct
     9901 gttccagtac gcgcagggaa tcactcacca ttttgcggtc gccgtccagg ctacgcggcg
     9961 aggtcatcgg cggtaagcgg aaggtaaaca tgccccacgc tgccggaacg acgatacctt
    10021 taccccccac ttctgccagc gcttcgagaa tgcgttcgat ctgctttaag ccattaagac
    10081 gacgctcttc gataaagtcg ccaatccaac cgtcatagcc accgcaggcg gtggtcaccg
    10141 gtaaaccggt ttctttgatt gctgctttga cttcttcgag attgttgacc agcagtttgc
    10201 cgtcaatctc aaaaccatcg aagcccatct ctttgatata acgaaatttc tccagaatgt
    10261 tttccggaaa gaatgcctga ttctgtgtgc ctattttcat tttttcattc ctttgaatta
    10321 aaacgtaacg cccattttga tgctctgttc cggatgctga tcgacatact gcatgtagct
    10381 ttccgggcta ttggcaaagg tcactaccgg gtcgattaaa tcttcgcaat tgagataacc
    10441 gttcatcagc agttcccagc aggtttcttc aatacgctta cggctccagc gcggataatc
    10501 cgggttcggt tcgctgcacg cgcgggagaa aacaattttg gcgttattga aatgcgcttc
    10561 gcgtccgagg ttaaaacctt cagcaaatgg tttggcaaac gcgacataag agatggtgcc
    10621 gccataagca aggccacgca gcgccgattg cagcgcgtcg gcgtaaccgc tggtttcgat
    10681 aatcacatcg gcaccctgct tgccggtcag cgttttgatc tcttttccta cgtcagtgcc
    10741 aatggggtta aggcagaaat cagcaccgtg gcgacgggca atatcacagc gataggcgat
    10801 aggatcgacg ccaatcacca ccgacgcgcc agcgcgtttc gccagttgga tggctatttg
    10861 accaatcgcg ccaagcccta ccaccaccac aaaatcaccc acgcgcacgt tggcatcgcg
    10921 cacgccgctc atggcaaact gcgccgggtc gtaacaaacg gcgtttttcc acgagctgcc
    10981 ttgcggcatt ttgcgcagct tgtagttatt caccgcgtta atgatgaccg tctcggagag
    11041 cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtcg gtaacgtcgc tgccgcactc
    11101 gataatgtcg ccaacctcca tgttgccaag ctggaatttg ccaaactcaa tgccgcgcgg
    11161 cgcatctgcc ggacgcggag tgaacatctg ccattcgccg ttaaagtctt catcgataaa
    11221 cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt ttcggtgcgc cgaaacgcac
    11281 gcggattttc acttcattgg ctaaaatcgc ccgatcttca tactcaacca gcgcagcaac
    11341 acgcggtgct gtggctacta actttttcat ttaaaactcc ttgtaaagaa actggccgct
    11401 ttagcccttc acgccaccgg cggtcaaacc actcttaata aaacgttccg aaagcgcata
    11461 cataatcacc accggcaatg cggtcaccag tgaggccgcc atcatccgtc cccagatgta
    11521 atctggcgta ctgaacagcg cgttcaggcc caccggtaag gtgaaattgc tggcgctgga
    11581 gaggaagatc gacgcaaaca gatagtcgtt ccacgccacc ataaagcagt agacgaacac
    11641 ggatatcagc ccggacatcg ccaacggcac ggtgatgcgg aagataattt gcagacggtt
    11701 gaggccatcc atcatcgccg cttcttcgat ctcatcgggg atggtgtcga agtagctttt
    11761 cagcataaac acggcggtgg gcagagtttg cgtcaccatg gtgatgatca gcgccatctc
    11821 agtgtcgtaa atgcccagcg cggtgatgat tttgaacagc ggcaccacca gcaaaatgcc
    11881 agagaacata tacaccgtgt aaaagctggc gttgatggtc atccgacctt taaagcgcag
    11941 gcgggaaagc gcgtaagccc caagaatgcc gaggaatacc gccaccacgg atgaaaccac
    12001 agacaccacc agactgttac ggaagtagtc gacaaacggg aaaatcatcg ggttaaaaat
    12061 gtcgacgtaa tgctccagcg tccactgctg cggcagcagc gtaggatgca gtgagatcgc
    12121 ctctttcgcg cccttgaacg aggtcatcag catcacaaaa aatggaaaca gcgtgatgat
    12181 gagaaacagc gccagcccgc agtaaaaacc gatgcgactg agtgtgcgtt tatttgttgc
    12241 cattgaggtt cacccgtttc ctggtcagca gaatgacagc gaaaataatg atgaagagca
    12301 ccaccgaaat cgccgccgct ttccctaagt cattaaaggc aaaggcggtt ttgtagagat
    12361 agacgccgag aatatcgacc ttggtggtca gcaaataaac atccgcgaac atgtagaaca
    12421 tccagatggt gcgcagggtc accaccgtcg ccaggaccgg cataatcgcg ggcagcgtga
    12481 cgatacgaaa ccgttgccag gcattagcgc catccatctc tgccgcttcg tacagcgatt
    12541 tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc atacgggaag tagcgccaga
    12601 tggcgaacag caccaccagc acaaagctac tgcccggatt atcgaaccac agcggtgcct
    12661 gctcatagag atgcagaaga tcgacgccga ggtagttcac aatgccgtag ccgttgttga
    12721 acatgtattt ccaggcgaac accagcgaaa tggacggcgt tacgtaggag aggatcacca
    12781 gcgaacgcgc ggttttgcgc aggcggaact cgcgattgaa aaacatcgcc accgccagcc
    12841 cgagcacggt gctgcccgcc accaccagcg cggtgtacca gaccgtcatc cacagtgagt
    12901 gccagaagcc gggatcggag aggatacgca catagttgct caccccaaca aaggttgact
    12961 cgatgttggg
//
LOCUS       DAEVZA010000138        12933 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEVZA010000138 DAEVZA010000000
VERSION     DAEVZA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.138"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..214
                     /locus_tag="LAF92_004080"
     CDS             <1..214
                     /locus_tag="LAF92_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793003.1"
                     /translation="FILFSWIFCYTTRFIYALKFAVAMTIANMGYQALCLYIDNSFAI
                     TRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(353..1174)
                     /gene="cobB"
                     /locus_tag="LAF92_004081"
     CDS             complement(353..1174)
                     /gene="cobB"
                     /locus_tag="LAF92_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBI9793004.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(1190..2101)
                     /gene="nagK"
                     /locus_tag="LAF92_004082"
     CDS             complement(1190..2101)
                     /gene="nagK"
                     /locus_tag="LAF92_004082"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBI9793005.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(2130..3374)
                     /gene="lolE"
                     /locus_tag="LAF92_004083"
     CDS             complement(2130..3374)
                     /gene="lolE"
                     /locus_tag="LAF92_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBI9793006.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(3374..4075)
                     /gene="lolD"
                     /locus_tag="LAF92_004084"
     CDS             complement(3374..4075)
                     /gene="lolD"
                     /locus_tag="LAF92_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBI9793007.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(4068..5267)
                     /gene="lolC"
                     /locus_tag="LAF92_004085"
     CDS             complement(4068..5267)
                     /gene="lolC"
                     /locus_tag="LAF92_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBI9793008.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            5529..6602
                     /locus_tag="LAF92_004086"
     CDS             5529..6602
                     /locus_tag="LAF92_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBI9793009.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLTLWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQQFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLGYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAISYSR"
     gene            6730..10176
                     /gene="mfd"
                     /locus_tag="LAF92_004087"
     CDS             6730..10176
                     /gene="mfd"
                     /locus_tag="LAF92_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBI9793010.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSKLPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENE
                     IA"
     gene            10320..11282
                     /gene="ldtC"
                     /locus_tag="LAF92_004088"
     CDS             10320..11282
                     /gene="ldtC"
                     /locus_tag="LAF92_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBI9793011.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(11365..11622)
                     /gene="bhsA"
                     /locus_tag="LAF92_004089"
     CDS             complement(11365..11622)
                     /gene="bhsA"
                     /locus_tag="LAF92_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBI9793012.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            11863..12495
                     /gene="comR"
                     /locus_tag="LAF92_004090"
     CDS             11863..12495
                     /gene="comR"
                     /locus_tag="LAF92_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBI9793013.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(12557..>12933)
                     /locus_tag="LAF92_004091"
     CDS             complement(12557..>12933)
                     /locus_tag="LAF92_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBI9793014.1"
                     /translation="NVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATV
                     VGALGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRM
                     DRDPGTQIPLDSNGQLILNNKV"
BASE COUNT         3149 a         3465 c         3252 g         3067 t
ORIGIN      
        1 gtttatctta ttttcctgga tcttttgcta cacgactcgt tttatttatg cgttaaaatt
       61 tgcagtggcg atgactattg ccaatatggg ttatcaggct ttatgtttgt atatcgataa
      121 tagctttgcg attaccagaa tatcgccact ctgggcaggg ctaattggtg tctgtacgtt
      181 tattgccgca ttgttattga cgagtaaacg ataaaagagg ccggaaaagt gcgcgtgtat
      241 tatgccggat ggggcgcgaa cgccttatcc ggcccacaaa acccgcaaat tcaattaatt
      301 gcgtcccctt gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc
      361 ccttcagcaa cttttcaaca aactcaggca ccacctggct tgccgggccg taatatttct
      421 cggcaaattc attaccgacc tgacttggtt cgagattcag ttccacggtg tgcgcgccat
      481 gcagtttcgc ttcgtgaaca aacccagccg ccggataaac atgcccggaa gtaccaatgg
      541 caatgaaaat atcagccatc gacaacgcca tataaatttc atccatgccg agtggcattt
      601 cgccaaacca aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat
      661 cttctggcgt aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt
      721 tcagcaattc cccatgcata tgaatgacat tggtattacc tgcacgttca tgcaggttgt
      781 ctatattctg cgttaccagc aaaaagcgat cgccgagggc gtcttgcagt ttagccagcg
      841 caagatgcgc ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat
      901 aaaacgcttg caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa
      961 cccgatgttc ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag
     1021 aaatccctgc ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt
     1081 tatctctgaa aaaaatacgc tgacgcaaac gctcgcgcag gcggcgttta tttttacgaa
     1141 aacgacttaa ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta
     1201 gatgtaggaa ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc
     1261 gcggaacacg agctacaggt aagagatgac gaggcagcct gtccgccagt tgcgttgtga
     1321 ttgccgggaa attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca
     1381 ggatatttcc cagacaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct
     1441 gctcatcgcc ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat
     1501 agtgttgata cagccacgca aaaccgcgac cagacagata attttcaatg cagccatgct
     1561 gaccacagcc gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg
     1621 gcagacgcat atggccaaac tcgccggtaa tgtagctttt accggtaatc ggtttgccgt
     1681 tgaaaatcag cccgccgcca acgccggtgc cgagaatcaa ccccatcacc agtggatatt
     1741 gagtaaattc atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc
     1801 gtacatcgcg atcaagacgt gcgctcaggt cggcacgcag cggtttaccg ctggcagcgg
     1861 ggacattggc ggcatacagc gtaccatctt ctgtttccgg cattcccgga ataccgatgc
     1921 cgacagaacc tttacagcca aaacgttggt cagcttcagc taccagctca cacactgcat
     1981 ctaaaaatgc gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc
     2041 gaccgctatc aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca
     2101 ttgccgctcc ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca
     2161 atattactgg cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg
     2221 accagtacgt agaagacgtc cagccagtgc aattccgatg gcaggaaatc aataaaatag
     2281 atatcgctgg agaggaactg atgaccgatc aacttttcaa tccactcaat aatcggggta
     2341 agttgcagtg aaaccaccac accgataatc accccacaca ggctgccgaa cagccctgcc
     2401 agcaatccat accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt
     2461 aatactgcga tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg
     2521 aaacaggcca cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg
     2581 atatcgcgat acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg
     2641 gtcacttcac ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg
     2701 gcaatacctg acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc
     2761 atggcaaaac tgtgatcgag ttgaccactc aactgcaaaa taccggcaac gtgcaaacgc
     2821 acacgttttg gctgcatcag tttatgctcg ggattcgagt tggggatcat aatcgacacc
     2881 caatccccct gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt
     2941 tcgcccgctt taaaattgcg ccacgcatcg ccctggacaa acgagggtaa tgcgctcaga
     3001 cgctgttcct gttgcgggtt aacgcccttc acctggattg cgcgaagatt cacgccactt
     3061 tccaccagcc cggtgaaatt gatatacggc gcggcagcgg caatacccgg cactttttgc
     3121 acgtgatcca gtgcttcctg ccagttagtc cacggctgat ccaccgcctc gatttcacca
     3181 tgcggcacca ccgccagaat acggttgttc agttcgcgtt caaagccgtt catcgcgctt
     3241 aagccgacga tcaataccgc cacgccgagg gcaatgccaa tggtagaaat aacggagatc
     3301 agcgacacca tgccgccgcg ccgccgtccg cgactaaaac gcaggccaat taataacgat
     3361 aaaggcatcg ccattactcc gcccccatca ggctcagttc cgccgtcaga cgaccatcac
     3421 gcatttctaa ctggcggctc atacgtttcg ccagttgcag gtcgtgagta accaccagga
     3481 aggcggtgcc ctgcaagcga ttcaattccc caagcaactg gaagatgctg tcggcgttac
     3541 gcgcatcgag gttaccggta ggttcatccg ccagtaccag gcgcgggtta ttcaccagcg
     3601 cacgggcaat cgccacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg
     3661 cacgatgatc cagccccacc gcttttaaca tctcaagtgc acggctgttg atttcagcgg
     3721 gctttttctt gccaatcagc agcggcatag ccacgttttc cagggcggta aaatccggca
     3781 gcaggtggtg aaactgataa ataaagccca gcttctggtt gcgcagctct gctttcgccg
     3841 ccgaagacag tttgctcatc ggttgaccgt taaagatcac atcgccggag gttggcgtat
     3901 ccagcccgcc caacaagtgc agcaaggtgc ttttaccgga accagagcta ccgacgatcg
     3961 ccatcatttc accttcgccg acgctgaaac tgacattgtg cagcacatcg gtttgcacac
     4021 tgccttcctg atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt
     4081 aaagcctcag cgggttgagt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc
     4141 gcgatagcca tcgccaccag cgcaataaca atgacctgta aaggttcgat agcgaccggc
     4201 agcgccgcgc catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca
     4261 agcagggcgc caagcgccgc tccgaggatc gcaccgataa tcccggcgct ggccccttgc
     4321 accataaaga ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgctact
     4381 tcgccctgct tctccattac catcagccct agtgaggtaa taatgttaaa cgccgcaacg
     4441 gcgacaatca ggctcagcag taaacccatc atattttttt ccatgcgtac ggcctggaac
     4501 aactcgcctt tacgatcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga
     4561 cttaatgagt cgactttcag cggctcatcc agccacaaac gccagccggt aatattgcct
     4621 gccggataac gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg
     4681 acttcactgt tggcggcgaa agtgccaatc acattgaaca ggcgctggct tggaatacgc
     4741 cccatcggcg tgaactggct ggcagatggt accatcacgc gaatttgatc accgcgatta
     4801 acgcctagct gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg
     4861 tcagtttgtt tcacattgac cagatacggt gtaagtggat ctttttgcgc cgggtcgata
     4921 ccgagcatca ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta
     4981 ataggtgcga cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg
     5041 ttaagagagc catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt
     5101 tgcagctcgc gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg
     5161 agggtaatgc cgatggtaga aagccaggag acgaaacgac cgaagcgatc cgctgcacgc
     5221 ccacgcatgt aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg
     5281 ttgctgtagc aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt
     5341 aagtgatcgt cagggctaat ctgaaaagcc cccgtgaatc aatcggctaa attgccatgt
     5401 tttatcatca atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac
     5461 ccattacaca tagggtcata acccgcagga tcaaccgcat gtaaattaaa aaagagcgtg
     5521 ttgtcctgat gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc
     5581 tggtggtgat ttatcactcg gtcattacct tttatccgca tctgaccact tttcagcatc
     5641 cgttatcgga agtcctgagc aaatgctgga tctatttcaa tctttacctt gcaccctttc
     5701 gtatgccggt ttttttcttt atttccggct atttgattcg ccgctatatc gacagcgtac
     5761 cgtggggaaa ttgtctcgat aaacgcatct ggagcatctt ttgggtgctg acgctttggg
     5821 gcgtggtgca gtggctggcg ttaagtgcgc ttaaccagtg gctggcacct gagcgcgatt
     5881 taagcaacgc ttccaatgcc gcgtatgccg attccaccgg tgagttcctg cacgggatga
     5941 tcacagccag caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta
     6001 aaatttttag ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg
     6061 ttaatttcgt tcccacgccg tggtggggaa tgaacagtgt gatccgcaat ttgccttatt
     6121 acagccttgg cgcatggttt ggcgcaacat taatgacctg tgttaaagag gtgccgttgc
     6181 gccgccatct gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta
     6241 ctatctcgct gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcaat
     6301 acgagcaaca attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca
     6361 ttgctatcta caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc
     6421 aaatgaacgc agcacgctgg tcgccgcaag tcgaactgac actcctgctg ggttacccct
     6481 ttgttagttt gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgcg
     6541 cattcagcga tctgttgttc tccccgcctt ctctgcccgc ggccatcagt tactcccgct
     6601 aagccatcgc cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga
     6661 acagcattgc ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg
     6721 acaaccgtta tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt
     6781 ctgctgggcg agttaaccgg cgctgcctgc gcgacgctgg tagcggaaat tgccgaacgt
     6841 cacgccggtc cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat
     6901 gaaatcagcc agtttaccga tcaaatggtg atgaatctgg cggactggga aactcttccc
     6961 tacgacagtt tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag
     7021 ctaccgacga tgcagcgtgg cgtactgatt gttccggtga atacgcttat gcagcgcgtt
     7081 tgcccgcaca gttttcttca cggtcatgcg ctggtgatga aaaaaggtca gcgtctgtca
     7141 cgagatgcat taagaaccca actggacagc gccggttatc gccatgttga ccaggtaatg
     7201 gagcacggcg aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtaag
     7261 ctgccttatc gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc
     7321 gacagccagc gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt
     7381 ccgaccgata aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg
     7441 aagcgcgatc cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgccgggatc
     7501 gagtactggc agccattgtt cttcagcgaa ccactgccgc cgctgttcag ttatttccct
     7561 gccaatacct tgctggtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct
     7621 gacacgctgg cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca
     7681 caatcgctct ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgggtg
     7741 cagctaaaaa ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa
     7801 ctgccagacc tggccgttca ggcacaacaa aaagcgccgc tggatgcgct gcgtaagttc
     7861 ctcgagactt tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg
     7921 ctgggtgaac tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa
     7981 gccagcgacc gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag
     8041 gtgcgtaatc tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt
     8101 cgtcaggatt ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg
     8161 catattggtc agccggtggt ccatctggag cacggtgtcg ggcgctatgc cggaatgacc
     8221 acgctcgaag cgggcggcat tactggcgag tatttgatgc tcacctatgc caacgacgcc
     8281 aaactgtatg ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa
     8341 gaaaacgccc cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg
     8401 gcggaaaaag tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc
     8461 aaagagggct tcgcgtttaa acacgatcgt gagcagtatc agttgttctg cgacagcttc
     8521 ccgtttgaaa ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt
     8581 cagccgctgg caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg
     8641 gcgatgcgcg ccgctttcct ggcagtagat aaccacaagc aggtggcggt gttggtgcct
     8701 accacccttc tcgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg
     8761 gtacgtatcg aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg
     8821 gaagtggcgg aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac
     8881 gtcaagttta aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt
     8941 cataaagagc gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg
     9001 ccgatcccac gcacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc
     9061 acgccgcccg cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg
     9121 gtccgggagg cgatcctgcg tgaaattttg cgcggggggc aggtttatta tctctacaat
     9181 gatgtggaaa acatccagaa agctgccgaa cggctggcag aactggtgcc tgaagcacgg
     9241 attgccatcg gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc
     9301 catcatcaac gtttcaacgt gctggtttgt accaccatta tcgaaaccgg gatcgacatc
     9361 ccgacagcca acaccattat cattgaacgt gcggatcact tcggtctggc gcagctgcac
     9421 cagttacgcg gtcgcgtcgg acgttcgcat catcaggcat atgcatggtt gctgacgccg
     9481 catccaaaag cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa
     9541 gatctcgggg caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa
     9601 ctgcttggcg aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag
     9661 ttgctggaaa acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc
     9721 accagccagc aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatt
     9781 cctgacgtga atacgcgttt gtcgttctat aaacgtattg ccagcgccaa aacggaaaac
     9841 gaactggaag agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc ggatccggcg
     9901 cgtaccctgc tggatgttgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag
     9961 ctggaaggta atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg
    10021 gcctggttga ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg
    10081 cgcctgaaat ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag
    10141 tttatgcgtg aactggaaga gaacgagatc gcttaatggt aaatcggggg cgtttctgcg
    10201 cccccatctg caacatttac aaattctttg cacttccctg cactatccga cactgtcacc
    10261 atccataatt caggcttatc tgtttattac aataacctta tatttattat ggatttttgg
    10321 tgatgatcaa aacgcgtttt tctcgctggc taacgttttt tacgttcgcc gctgccgtgg
    10381 cgctggcgct accggcaaaa gccaacacct ggccgctgcc gccagcgggc agtcgtctgg
    10441 ttggcgaaaa caaatttcat gtggtggaaa atgacggtgg ttctctggaa gccatcgcca
    10501 aaaaatataa cgtcggcttt ctcgctctgt tacaggctaa ccccggcgtt gatccttacg
    10561 taccgcgcgc gggcagcgtg ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc
    10621 gcgaaggcat tgtgatcaac attgcggagc tgcgtctcta ttactacccg ccgggtaaaa
    10681 attcggtaac cgtgtatcca ataggtattg gtcagttagg tggtgacacg ctgacaccga
    10741 caatggtgac caccgtttca gacaaacgtg caaacccaac ctggacgcca acggcaaaca
    10801 tccgcgcccg ttataaagca cagggaattg agttgcctgc ggtagtgccg gctggaccgg
    10861 ataacccaat gggccatcat gcgattcgtc tggcggccta tggcggcgtt tatttgcttc
    10921 acggtacgaa cgccgatttc ggcattggca tgcgggtaag ttctggctgt attcgtctgc
    10981 gggatgacga tatcaaaaca ctctttagcc aggtcacccc aggcaccaaa gtgaatatca
    11041 tcaacactcc gataaaagtc tctgccgaac caaacggtgc gcgtctggtt gaagtacatc
    11101 agccgctgtc agagaagatt gatgacgatc cgcagctgct gccaattacg ctgaatagcg
    11161 caatgcaatc atttaaagat gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg
    11221 atgtccgttc cgggatgccg gtggatgtcc gccgtcatca agtgagccca caaactctgt
    11281 aaatcctgga taaaaaaaat ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta
    11341 gcaggcatta atgagggtta atgcttattt ataaattact gctgttccat ggagggtatt
    11401 cggaccggtt acagaagtaa tacggaaaga ttttgcgccc atctcatccg ctttttgcgc
    11461 cagctgctct tccagcgatc ccagatttgt ccccgcgtta gcactgattg taccgacttt
    11521 ttgttggcct tctggcgttg actgaacttc gacagccgca aagctggcaa atgacatgga
    11581 gcttaaaatc gccgcagcga tgagggtttt tacgtttttc ataatagtgg ccttatgcag
    11641 atgaatgacg tcgcaagcaa ttacttaacg atcgataact aaatgataga tgtgatctgg
    11701 atcacataca agatattttt tataacaatc attaattaat ttaaaaatct ttaaatttca
    11761 gttaaataga aagcaattat ttttatggga tctggactgg tgaacggagg tgattatttt
    11821 tataataacc tttcattcac agtcaccagg tacaacggca tcatggcaac tgactcaaca
    11881 caatgtgtaa aaaaaagccg tggccgccca aaagtgttcg acagggatgc cgcgcttgat
    11941 aaggccatga aattgttctg gcaacacggt tatgaagcga cttctcttgc ggacctcgtc
    12001 gaagcgaccg gagccaaagc gcccacgcta tacgcggaat ttaccaacaa agaggggtta
    12061 tttcgtgccg ttctcgaccg ctatatcgat cgttttgccg ctaagcatga agcacagctg
    12121 ttttgtgaag agaaaagcgt ggagtctgcg ctggctgact attttgctgc catcgccaac
    12181 tgctttacca gcaaagacac cccggcaggc tgcttcatga tcaacaactg caccaccctc
    12241 tccccagatt caggagatat cgccaatacg ttgaaatcac gccatgcgat gcaagagcgc
    12301 actttgcagc agtttttatg tcaacgacaa gcgcgcgggg aaatcccgac ccactgtgac
    12361 gtgacacatc tggcagaatt ccttaattgt attattcagg ggatgtcgat cagcgcacgc
    12421 gaaggtgcat cgctggaaaa actaatgcag attgccggaa cgactttgcg tttatggccc
    12481 gaactggtga aataagtcgc aaaaaaagcc cctcagcctg agcgaatgag gggccaaatt
    12541 gcagagtaca gccttgttat actttgttat tcaaaatcag ttgcccatta ctatctagcg
    12601 ggatctgcgt acccggatcg cggtccatgc ggattttgcc ctgctgatcg ccaatcttat
    12661 aggtcacatc ataaccgagc attttttctg acttgtcata caccgtttta caacgctgtt
    12721 gcgtagtcgt gtaagtatcg ctttcctgga gagagccctg gatctggtta ccggcatatc
    12781 caccacccag cgcccccaca acagtggcga catctttacc gcgaccaccg ccaaactgat
    12841 gccctatcac gccgccagca acagcgccga gcaccgaccc ggtaatgcga ttttcatcct
    12901 gcaccggtcg acgatgggtc actgtgacgt tgc
//
LOCUS       DAEVZA010000139        12755 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEVZA010000139 DAEVZA010000000
VERSION     DAEVZA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.139"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(277..1221)
                     /locus_tag="LAF92_004092"
     CDS             complement(277..1221)
                     /locus_tag="LAF92_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI9793015.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            1745..3277
                     /gene="pntA"
                     /locus_tag="LAF92_004093"
     CDS             1745..3277
                     /gene="pntA"
                     /locus_tag="LAF92_004093"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBI9793016.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            3288..4676
                     /gene="pntB"
                     /locus_tag="LAF92_004094"
     CDS             3288..4676
                     /gene="pntB"
                     /locus_tag="LAF92_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBI9793017.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(4701..5735)
                     /gene="tqsA"
                     /locus_tag="LAF92_004095"
     CDS             complement(4701..5735)
                     /gene="tqsA"
                     /locus_tag="LAF92_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBI9793018.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            6147..6512
                     /gene="mdtJ"
                     /locus_tag="LAF92_004096"
     CDS             6147..6512
                     /gene="mdtJ"
                     /locus_tag="LAF92_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBI9793019.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            6499..6828
                     /gene="mdtI"
                     /locus_tag="LAF92_004097"
     CDS             6499..6828
                     /gene="mdtI"
                     /locus_tag="LAF92_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBI9793020.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(6867..7688)
                     /locus_tag="LAF92_004098"
     CDS             complement(6867..7688)
                     /locus_tag="LAF92_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBI9793021.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(7788..7871)
                     /locus_tag="LAF92_004099"
     CDS             complement(7788..7871)
                     /locus_tag="LAF92_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793022.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(7964..8299)
                     /locus_tag="LAF92_004100"
     CDS             complement(7964..8299)
                     /locus_tag="LAF92_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBI9793023.1"
                     /translation="MKKQIEGMTMKKVLGLVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            complement(8696..9949)
                     /locus_tag="LAF92_004101"
     CDS             complement(8696..9949)
                     /locus_tag="LAF92_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9793024.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLIGTRLHRRLHA"
     gene            10056..10949
                     /locus_tag="LAF92_004102"
     CDS             10056..10949
                     /locus_tag="LAF92_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9793025.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            11084..12304
                     /gene="mlc"
                     /locus_tag="LAF92_004103"
                     /gene_synonym="dgsA"
     CDS             11084..12304
                     /gene="mlc"
                     /locus_tag="LAF92_004103"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBI9793026.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            12429..>12755
                     /locus_tag="LAF92_004104"
     CDS             12429..>12755
                     /locus_tag="LAF92_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9793027.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHV"
BASE COUNT         3208 a         3033 c         3310 g         3204 t
ORIGIN      
        1 ccatattttg cggcagactg aaaacacccg cgcccagcat tgagcttaat accagcgcgg
       61 tgagtgcgct cagtcccagt ttcttttcca tcgatatcca gtgattaaaa cgtgataaga
      121 agaataaata ttagggcaaa acgcataaaa atggagtttg ccactgaagc gcgggatttt
      181 acgaagagat gtgtctgcat gcaatggtcg cggggaaatt tgtgactaaa aatgcgaaag
      241 gcggcatcaa gccgccttat aggagtaact gaatagttat ttatacagat ctgcgctgac
      301 ggtcaggtta ttaccacgtt cctgccactg gcgggtgatg tggtaatact tagcaccttt
      361 cttcgcggca cgtttcgcaa cctgatagga gacttcggtc atgttgccgt agttgccaga
      421 gaatttgatg ctgtcgaacg ggaccatcat cgctgctgtc gctttgttca gttcttcgac
      481 tttagtgccg tcaggaagcg tgacagtgta acgtccgcct tttgatgact gggtttcaaa
      541 gaaacgaccg acttcagaac ttggtgatgc ggtagtcgca acacctggga tctcaacttt
      601 cttcgcggct tcgccaccgg cagccagagc tgcacgtcct gcttcggaat ctgccgggat
      661 cacatccggg ctctggacga tacgtttctt agcatctttt ttatagatga atgcagtaat
      721 acgctggttg ccgccctggt tggcatcgat ttgacgaacg atgtagaaag agtaggcacc
      781 tttcgctttt gccgctttgg tgatggcatc attgacttct ggctggctac gatagaagcc
      841 ctggacggtg accgtgtcaa acggttcaat cagaacagcc tgatctttcg gcagttcgac
      901 aacaccgtta attacgcgat tacttgtttc ttctgctttt tcagcatcgg ctttatagag
      961 gtcagcgacc acacgccagt taccgctgtt accaaaatca gaagtgtcga caacataaaa
     1021 agaggcggca ccttctttat ctgcgcgacg agaaacggct ttcaccgctt cgccaatagc
     1081 attaaaacga ccggtaacca ctacacggtc aaaaggttta accgctgccg cttgctccgg
     1141 tgtcagttct gttgctgcgt taacggagaa tgccgtagca gaaagcagtg ccgacgccag
     1201 gagggtgttc ttaagcttca taaaaataat ccttcgcctt gcgcaaacca ggtactggta
     1261 ttgttattaa cgagaaacgt ggctgattat tgcatttaaa cggtgtaact gtctgcgtca
     1321 tttttcatat cacattcttt aagccaattt taatcctgct caaatgaccg tctatgctta
     1381 aaaaacagcc gtatcagcat cattactact gaagcaactg aattatataa gttaatttaa
     1441 tgttaagtag tgattcgtgc cggggcgatg tctcgtttta cccgaccgtc gaagacaatt
     1501 atcagtcttt atccggcgtt ctaaggtgtt tatcccacta tcacggctga atcgttaata
     1561 ttttgcgagt tcacgccgaa atactgattt ttggcgctag atcacaggca taattttcag
     1621 tacgttatag ggcgtttgtt actaatttat tttaacggag taacatttag ctcgtacatg
     1681 agcggcttgt gtggctcctg acacaggcaa accatcatca ataaaaccga tggaagggaa
     1741 tatcatgcga attggcatac caagagaacg gttaaccaat gaaacccgtg ttgcagcaac
     1801 gccaaaaaca gtggaacagc tgctgaaact gggttttacc gtcgcggtag agagcggcgc
     1861 gggtcaactg gcaagttttg acgataaagc gtttgtgcaa gcgggcgctg aaattgtaga
     1921 agggaatagc gtctggcagt cagagatcat tctgaaggtc aatgcgccgt tagatgatga
     1981 aattgcgtta ctgaatcctg ggacaacgct ggtgagtttt atctggcctg cgcagaatcc
     2041 ggaattaatg caaaaacttg cggaacgtaa cgtgaccgtg atggcgatgg attctgtgcc
     2101 gcgtatctca cgcgcacaat cgctggacgc actaagctcg atggcgaaca tcgccggtta
     2161 tcgcgccatt gttgaagcgg cacatgaatt tgggcgcttc tttaccgggc aaattactgc
     2221 ggccgggaaa gtgccaccgg caaaagtgat ggtgattggt gcgggtgttg caggtctggc
     2281 cgccattggc gcagcaaaca gtctcggcgc gattgtgcgt gcattcgaca cccgcccgga
     2341 agtgaaagaa caagttcaaa gtatgggcgc ggaattcctc gagctggatt ttaaagagga
     2401 agcgggcagc ggcgatggct atgccaaagt gatgtcggac gcgttcatca aagcggaaat
     2461 ggaactcttt gccgcccagg caaaagaggt cgatatcatt gtcaccaccg cgcttattcc
     2521 aggcaaacca gcgccgaagc taattacccg tgaaatggtt gactccatga aggcgggcag
     2581 tgtgattgtc gacctggcag cccaaaacgg tggcaactgt gaatacaccg tgccgggtga
     2641 aatcttcact acggaaaatg gtgtcaaagt gattggttat accgatcttc cgggccgtct
     2701 gccgacgcaa tcctcacagc tttacggtac taacctcgtt aatctgctga aactgttgtg
     2761 caaagagaaa gacggcaata tcactgttga ttttgatgat gtggtgattc gcggcgtgac
     2821 cgtgatccgt gcgggcgaaa ttacctggcc ggcaccgccg attcaggtat cagctcagcc
     2881 gcaggcggca caaaaagcgg caccggaagt gaaaactgag gaaaaatgtg cctgctcacc
     2941 gtggcgtaaa tacgcattga tggcgctggc aatcatcctt ttcggttggc tggcaagcgt
     3001 tgcgccgaaa gagtttcttg ggcacttcac cgttttcgcg ctggcctgcg ttgtcggtta
     3061 ttacgtggtg tggaatgtat cgcatgcgct gcatacaccg ttgatgtcgg tcaccaacgc
     3121 gatttcaggg attattgttg tcggagcact gttgcagatt ggccagggcg gctgggttag
     3181 cttccttagt tttatcgcgg tgcttatagc cagcattaat attttcggtg gcttcaccgt
     3241 gactcagcgc atgctgaaaa tgttccgcaa aaattaaggg gtaacatatg tctggaggat
     3301 tagttacagc tgcatacatt gttgccgcga tcctgtttat cttcagtctg gccggtcttt
     3361 cgaaacatga aacgtctcgc cagggtaaca acttcggtat cgccgggatg gcgattgcgt
     3421 taatcgcaac catttttgga ccggatacgg gtaatgttgg ctggatcttg ctggcgatgg
     3481 tcattggtgg ggcaattggt atccgtctgg cgaagaaagt tgaaatgacc gaaatgccag
     3541 aactggtggc gatcctgcat agcttcgtgg gtctggcggc agtgctggtt ggctttaaca
     3601 gttatctgca tcatgacgcg ggaatggcac cgattctggt caatattcac ctgacggaag
     3661 tgttcctcgg tatcttcatc ggggcggtaa cgttcacggg ttcggtggtg gcgttcggca
     3721 aactgtgtgg caagatttcg tctaaaccat tgatgctgcc aaaccgtcac aaaatgaacc
     3781 tggcggctct ggtcgtttcc ttcctgctgc tgattgtatt tgttcgcacg gatagcgtcg
     3841 gcctgcaagt gctggcattg ctgataatga ccgccattgc gctggtattc ggctggcatt
     3901 tagtcgcctc catcggtggt gcagatatgc cagtggtggt gtcgatgctg aactcgtact
     3961 ccggctgggc ggctgcggct gcgggcttta tgctcagcaa cgacctgctg attgtgaccg
     4021 gtgcgctggt cggttcttcg ggcgctatcc tttcttacat tatgtgtaag gcgatgaacc
     4081 gttcctttat cagcgttatt gcgggtggtt tcggcaccga cggctcttct actggcgatg
     4141 atcaggaagt gggcgagcac cgcgaaatca ccgcagaaga gacagcggaa ctgctgaaaa
     4201 actcccattc agtgatcatt actccggggt acggcatggc agtcgcgcag gcgcaatatc
     4261 ctgtcgctga aattaccgag aaactgcgcg ctcgtggtat caacgtgcgt ttcggtatcc
     4321 acccggttgc ggggcgtttg cctggacata tgaacgtatt gctggctgaa gcaaaagtac
     4381 cgtatgacat cgtgctggaa atggacgaga tcaacgatga ctttgctgat accgataccg
     4441 tactggtgat tggtgctaac gatacggtta acccggcggc gcaggatgat ccgaagagtc
     4501 ctattgctgg tatgcctgtg ctggaagtgt ggaaagcgca gaacgtgatt gtctttaaac
     4561 gttcgatgaa cactggctat gctggtgtgc aaaacccgct gttcttcaag gaaaacaccc
     4621 acatgctgtt tggtgacgcc aaagccagcg tggatgcaat cctgaaagct ctgtaaccct
     4681 gacggcctct gcggaggccg tcactcttta ttgagatcgc ttaacagaac ggcgatgctt
     4741 tgacctcccg ctgtttgttc aagcgcaatt ttgacaataa ttgttaacgg cacggaaagc
     4801 agcataccca ccggtcctaa caaccatccc caaaaaatca acgacaaaaa taccaccaat
     4861 gtggaaagcc ccagcccacg tcccatgatg cgcggctcca gaatattgcc gaagaccaga
     4921 ttaatcagca gatatcccgc cagcaccagc aacgcttcgt agaagccatt aaacaccagt
     4981 acctgagcga taggggggat tgccgcgaga actgaaccaa tattagggat gtaattaagc
     5041 gcaaaggcca gcaatcccca gacaaaagcg aagcgaacat cgagtgcggc gagcatcgcc
     5101 caggcgacca ggccggtgat gatgctgatg gctgttttta gcaccagata atgagaaaca
     5161 ctgtcaattg cacgttgaat cgccgccatc ccttcaaccg gacgcgccat catttgctga
     5221 aattttccgg gcaattgtgg cacttcgagc agcataaaca gcaccgtcag cagcaataaa
     5281 aatattgatg acatggcatt agataactgc gtcaataagt tggtgagcaa cgtcatcgcc
     5341 gcgttcggat caatgtaatg cgccagctgg tcaactgaga cgtcaatccc tacgcgttgc
     5401 aacaacggct caagagcttg cagcggcgtc ataatagagt tgcgatattg cggtaacgtc
     5461 cgcgtcaact cgttgagcgc ggaacccaga taagctaata gcaacaccat cgccatcacg
     5521 atgatggtca tcaaaatcga caccgccagt acacgcggca ctcgccagcg gaccatgtgt
     5581 tgcaccagcg ggttaagaat aacagcaata aataatgcga gaataaacgg cacgatgatc
     5641 tcggcggcaa aacggatacc gcagagaata atgaccagca ttcccaacat aatgacgatt
     5701 tttaggccat tgagcgtgat gatcggcttt gccatgcttg tccccgtttt ttcttccttt
     5761 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
     5821 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
     5881 ccgaggacaa ttatcatccg cgatgacgag aagcaacact gcggataatt gtaatattat
     5941 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
     6001 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
     6061 tgccctatgc gcggggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
     6121 aataattctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
     6181 ttgctacaga aattaccggt acgctgtcca tgaaatgggc gagcgtcagt gagggaaatg
     6241 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
     6301 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
     6361 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
     6421 taaccaccct ggtcgccggg attgtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
     6481 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
     6541 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
     6601 aaaatatttg gcttgctctc cctggcggcg gtgctggctg cctttagtgc gctttctcag
     6661 gccgtaaaag ggatcgactt gtctgtcgct tatgcattgt ggggcgggtt tggtattgcc
     6721 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
     6781 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aacacgctgc
     6841 ccgcactgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
     6901 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
     6961 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
     7021 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
     7081 acttcacagt tttgatgact gtataacgta tcgagatgat cttcaggata tcccgcctga
     7141 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
     7201 aacggcaacg gtgtaatgcc agaaggggga ttacgcagca caatcaagcc gaagtcccat
     7261 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
     7321 gtcggatcaa cgcggccttc tatgtcgtgg atgtcatagc gccaaagacc tttatttgac
     7381 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
     7441 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
     7501 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
     7561 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
     7621 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
     7681 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
     7741 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
     7801 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
     7861 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
     7921 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
     7981 cagcaggttt tgccggttgc tgatggctgt gtttcccggc gtgtttcttc gctgcctgcg
     8041 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
     8101 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgccggcg
     8161 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
     8221 cagaagacag acccatagca gcggcaacaa ccagacctaa tacttttttc attgtcatac
     8281 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
     8341 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
     8401 taaattacag cattgataat aacgtattgt gacttaaggg aaatttagct accaataata
     8461 gtagtcttga tcgggttaac tattttacta cttacaaagc ggtagaataa ctgcgcatca
     8521 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
     8581 gctagacgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
     8641 ggtttgagca agcaataagt ccggacgggt atttaccgca gtccgggctt atttttcagg
     8701 cgtgcagacg acgatgcaaa cgcgtcccga tcagcagagc aatgaccagc atcagagcaa
     8761 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
     8821 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
     8881 gttttgcgcg ggggccaatc cagctgctgg caactgagtg ggctgcgaag aatcctgctg
     8941 agaagagtaa cattccggca aagatcagcc acagcgagct gaataaggtc atcagtaaac
     9001 caaacagcat aacccccgtc gaaaacaaca tcaccggacc gcgcccgtag cgggtggtca
     9061 tggttccggc tttaggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
     9121 ccacggcctg actgacatgc cagggggaaa gcatcaaccg atagccgatg taattaaaca
     9181 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
     9241 cacgccagtg cagacgaaag ttgataaaca acgtcttagg tcgcagcgaa gtcgggcgaa
     9301 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggctagc gcgaaacaac
     9361 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
     9421 gcccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
     9481 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
     9541 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
     9601 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
     9661 catcggatag ggggccagta aacagcaaac caatagccaa catcgccgtg gaaatggaca
     9721 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
     9781 gctgcacaca atagagaagt gcaaatgttg ccagtccggc agagaacagc gccagggtga
     9841 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
     9901 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
     9961 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    10021 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    10081 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    10141 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    10201 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    10261 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    10321 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    10381 tgtctgatac gttatcgctg tttcgccgtg attatcccga tgtccattta caaacccgcg
    10441 aaatgaacac tcgtgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    10501 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    10561 tggcgatgat cccgcacgat catcccctgg caaataaccc gaatgtaacg ctggctgaac
    10621 tggcgaaaga acccttcgtc ttttttgatc cgcacgtcgg gacagggctg tatgacgata
    10681 ttctcgggct gatgcgacgt taccatttga cgcccgtcat cactcaggag gtgggcgagg
    10741 caatgaccat catcggtctg gtttccgccg gtctgggtgt ttcaattttg cctgcgtcat
    10801 ttaaacgtgt tcagctcaac gaaatgcgct gggtgccgat tgcagaagag gatgcggttt
    10861 ctgaaatgtg gttggtctgg ccgaaacatc atgaacaaag tccagctgcg cgtaactttc
    10921 gtattcatct gctgaatgct ctcaggtgag ggaaatttca gcgaaaaagc ccgaaaaatg
    10981 tgctgttaat cacatgccta agtaaaaatt tgacgacacg tattgaagtg cttcaccata
    11041 gcctacagat tatttcggag cgcgaaaata tagggagtat gcggtggttg ctgaaaacca
    11101 gcctgggcac attgatcaaa taaagcagac caacgcgggc gcggtttatc gcctgattga
    11161 tcagcttggt ccagtctcgc gtatcgatct ttcccgtctg gcgcaactgg ctcctgccag
    11221 tatcactaaa attgtccgtg agatgctcga agcacacctg gtgcaagagc tggaaatcaa
    11281 agaagcgggg aaccgtggcc gtccggcggt ggggctggtg gttgaaactg aagcctggca
    11341 ctatctttct ctgcgcatta gtcgcgggga gattttcctt gctctgcgcg atctgagcag
    11401 caaactggtg gtggaagagt cgcaggaact ggcgttaaaa gatgactcgc cattgctgga
    11461 tcgtatcatt tcccatatcg atcagttttt tatccgccac cagaaaaaac ttgagcgtct
    11521 aacttcgatt gccataaccc tgccgggaat tattgatacg gaaaatggca ttgtacatcg
    11581 catgccgttc tatgaggatg taaaagagat gccgctcggc gaggcgctgg agcagcatac
    11641 cggcgttccg gtttatattc agcatgatat cagcgcatgg acgatggcag aggccttgtt
    11701 tggtgcctca cgcggggcgc gcgatgtgat tcaggtggtt atcgatcaca acgtgggggc
    11761 gggcgtcatt accgatggtc atctgctaca cgcaggcagc agtagtctcg tggaaatagg
    11821 ccacacacag gtcgacccgt atgggaaacg ctgttattgc gggaatcacg gctgcctcga
    11881 aaccatcgcc agtgtggaca gtattcttga gctggcacag ctgcgtctta atcaatccat
    11941 gagctcgatg ttacatggac aaccgttaac cgtggactca ttgtgtcagg cggcattgcg
    12001 cggcgatctg ctggcaaaag acatcattac cggggtgggc gcgcatgtag ggcgcattct
    12061 tgccatcatg gtgaatttat ttaacccaca aaaaatactg attggctcac cgttaagtaa
    12121 agcggcagat atcctcttcc cggtcatctc agacagcatc cgtcagcagg cccttcctgc
    12181 gtatagtcag cacatcagcg ttgagagtac tcagttttct aaccagggta cgatggcagg
    12241 ggctgcgctg gtaaaagacg cgatgtataa cggttctttg ttgattcgtc tgttgcaggg
    12301 ttaacatttt ttaactgttc taccaaaatt tgcgctatct caatttgggc caggaaagca
    12361 taacttagac tttcaaggtt aattattttc ctggtttata tttgtgaagc ataacggtgg
    12421 agttagtgat gctgaagcgt ttctttatta ccggtacaga cacttctgta gggaaaacgg
    12481 tggtttcccg cgcattgcta caagcgttag cctcccaggg aaaaacggtt gcgggatata
    12541 aacccgtagc gaaggggagc aaagagacac ccgaagggct gcgtaataaa gatgccctgg
    12601 tgttgcagag tgtttcaacc atcgaactgc cttatgaagc agttaatcct atcgcgttaa
    12661 gcgaagaaga aagtagcgtg gcgcacagtt gcccaatcaa ttacaccctc atttcaaacg
    12721 gcctggcaaa cctgaccgat aaagtcgatc atgtc
//
LOCUS       DAEVZA010000140        12629 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEVZA010000140 DAEVZA010000000
VERSION     DAEVZA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.140"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..277)
                     /locus_tag="LAF92_004105"
     CDS             complement(<1..277)
                     /locus_tag="LAF92_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBI9793028.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNI"
     gene            complement(297..1523)
                     /gene="allC"
                     /locus_tag="LAF92_004106"
     CDS             complement(297..1523)
                     /gene="allC"
                     /locus_tag="LAF92_004106"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBI9793029.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPHPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLA"
     gene            complement(1545..2594)
                     /gene="allD"
                     /locus_tag="LAF92_004107"
     CDS             complement(1545..2594)
                     /gene="allD"
                     /locus_tag="LAF92_004107"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HBI9793030.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            2911..4578
                     /locus_tag="LAF92_004108"
     CDS             2911..4578
                     /locus_tag="LAF92_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBI9793031.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            4588..5847
                     /locus_tag="LAF92_004109"
     CDS             4588..5847
                     /locus_tag="LAF92_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBI9793032.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            5858..6673
                     /locus_tag="LAF92_004110"
     CDS             5858..6673
                     /locus_tag="LAF92_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBI9793033.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            6670..7563
                     /locus_tag="LAF92_004111"
     CDS             6670..7563
                     /locus_tag="LAF92_004111"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBI9793034.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7700..8767)
                     /gene="purK"
                     /locus_tag="LAF92_004112"
     CDS             complement(7700..8767)
                     /gene="purK"
                     /locus_tag="LAF92_004112"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBI9793035.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(8764..9273)
                     /gene="purE"
                     /locus_tag="LAF92_004113"
     CDS             complement(8764..9273)
                     /gene="purE"
                     /locus_tag="LAF92_004113"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBI9793036.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            complement(9391..10113)
                     /gene="lpxH"
                     /locus_tag="LAF92_004114"
     CDS             complement(9391..10113)
                     /gene="lpxH"
                     /locus_tag="LAF92_004114"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBI9793037.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(10116..10610)
                     /gene="ppiB"
                     /locus_tag="LAF92_004115"
     CDS             complement(10116..10610)
                     /gene="ppiB"
                     /locus_tag="LAF92_004115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBI9793038.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            10784..12169
                     /gene="cysS"
                     /locus_tag="LAF92_004116"
     CDS             10784..12169
                     /gene="cysS"
                     /locus_tag="LAF92_004116"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBI9793039.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(12205..>12629)
                     /locus_tag="LAF92_004117"
     CDS             complement(12205..>12629)
                     /locus_tag="LAF92_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI9793040.1"
                     /translation="LAMLPDADVLSFKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLI
                     GRRWFRAGLIRCWLFLTVSLLSHSLLDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAP
                     FALSRYTTPYGHQVIISELMWVWLPGMVLMGMLWWRRR"
BASE COUNT         2897 a         3332 c         3493 g         2907 t
ORIGIN      
        1 tgatatttcc agagatcaca tacaggaacg tttcaatgcc ttcgccaccg aagccctgtt
       61 ggttgccacc gttttgatgc agtgtgacca gataatcaac aaaagaggca cccagctttg
      121 gcgtggagag gattgtcgcg tcacaatttt caaagcccgg aataatattt tttaccagac
      181 catccggggt taacagtgcg aaattaccgt gtttaacaat cgcacggtta gccagtaaat
      241 cttcgcggta accggtgacg ttatttaaat atcccattta tgactcctta tttctgctag
      301 gcaagttgat aaagcatgag tgccaacgtt ttgacccctt cggcaaggtc ggtaatattg
      361 gtgcgttccg ccgggttatg gctgatcccg ttgatgctgg ggataaaaat catgcaggtt
      421 ggtacgcgag gcgcgaaaat ttgcgcgtcg tgcccggcac cactgtgcat cacccggtaa
      481 ttcagttttt ctctttcaca caattctgtc agggtggcga ccagctcctt attcatcggc
      541 acgggttctt cgtccatcca taaatcgata tcaataccaa tgtccatttc atcgcaaatc
      601 gcccgcatgt cgttttctaa ctgttgggtg aaatcgcgca gcacggcagc gtcggtatga
      661 cgacaatcaa tggtgaacgt ggttttaccc ggcaccacat ttaccgtatt cgggtgcggc
      721 tctactttgc caaaggtcag aaccagcgga tcgcccatcc ttttcgcttt ttcgaccgac
      781 tgatggcaaa tgcgactgaa agcgtaaact gtatcacgac gataacccat cggcgtggtg
      841 cctgcatggt ttgattcgcc gttcagcgtt accgtataac gacgctgccc gacaattgca
      901 ttcaccacgc caattgattg cccattactt tccagcacac agccctgttc aatatgcagt
      961 tcaacaaagg ctttaatatc ctgacgcgga gttagtgggg cgttcggaag agtaaatccg
     1021 caagccttca tcgcatcgac aaaactattt cctttagcat cacagatatt ccgcacgtcg
     1081 tcaggattcg ccagcccaaa gatattttta ctgccccaga agacatacgg gaagcggctg
     1141 ccttcttctt ctgccatcgc caccacttcg accgtacgta gcggcgcgcc gtattgcgtt
     1201 ttcagccagt caattgccag ccacgccgcc agcgcgccga attgcccgtc gaggttaccg
     1261 ccgttaacca cggtatcgat atgcgaaccg ctcagaacca cttcttgtgg atattcggtg
     1321 ccactcaggc gaccatataa attccccact tcatcgaaac gtgtttccag cccgcttgct
     1381 gccattcttt ttttaaattg ctgctgagtt tccagccatt ccggcgaata aagtaaacgg
     1441 gtcataccac ccgttgggtc agcgccaaaa gaggaaagcc agggcagcgt ttcttctatg
     1501 gcttgacgaa aatgtgtaat cataagaaag tcctgtctca ataattattg cgcaaaggga
     1561 tttttcgttt cgtatgacgt gttataaagc gcgtcggaaa ttaaatactg gtaaatatca
     1621 tcaacaattt cgatgccttc gacggcggct ttgcgttgtt taatatcctg atcctgtccg
     1681 ggataataaa cctgattaaa accgggcgcg ggggtaatgg catttaattc gcgcatggtc
     1741 tggctaagat gttgacgaaa taatttgctg gagaagaaaa agttcgggtt aataacgata
     1801 tgtaattgcc ccaaattacg cccggcgtgt aaatcgtcat acatcgaact aacctgtcgc
     1861 ccgaacggta agccgagtaa gacgcctgag aggacgtcaa tcatcatcat caggccatac
     1921 ccttttggcc cggcggcggg gagcagagca tgtaccgcga acggatcggt tgttggtgca
     1981 ccgtttttat cgaccgccca ggtatccggg atagacatat tacgcgagcg ggcgtcgagc
     2041 acttttcccc atgcctgtac ggtagtcgcc atatcaaagg taaggatctc gtcgccttct
     2101 cccggcgcgg caaaggccag tgggttagta ccgtagtaaa tttccgcgcc gccaaacggc
     2161 accaccattg gatcggactg gcacatcgaa ataccaatta atccggcgcg ggctgcctgc
     2221 tgcacaaaat aagagattgc gccgctgtga cccatccggc tgataccgac caccgcaacg
     2281 ccattttgct gggcggtttt gatggcatgt tccataccca ttttcgccgc gacctgtccg
     2341 gcggcattgt cggcatgtaa aattgccgag cacggcccgg tttcctcaag acgaaactcc
     2401 ggttcgcggt tggtgccgcc ttttgaaatg cgttccgcgt agtattccac gcgcaccgcg
     2461 ccatgagagt ggatccctct ggcatcggcg taaaccaata cttcagccac ggttgcagcg
     2521 tgctcacgtt ttaacccagc ctggcagagt ttattctcaa ttagctggtg gagtgtttcc
     2581 cgactgattt tcatctgtct tcctttttaa cgacggtgtg aagcatgact gcaattaaca
     2641 tacagggaaa atatctggat tatgtgatcc agacaggcaa aaaaatatag ttagaattta
     2701 tttgataatc cgctcacttt taacctgatt tttaaaacaa ccacgcttat taaaaaataa
     2761 tgcgtaatag cctggcggtt atttgaattc ttttgttaat aatccctgtg tgatattcat
     2821 caccttattt actcgttgtc atcgataccg taatcgccac attaacactg ctcgtgcaat
     2881 tgccatgggt gcaattttta aggagttgtt atgatccacg cctttattaa aaaagggtgt
     2941 tttcaggatt cggtcagttt aatgattatt tcacgaaaac tcagcgaatc agaaaatgtt
     3001 gatgatgttt ccgtaatgat gggtacgccc gccaataaag cgttattaga taccacaggt
     3061 ttctggcatg acgattttaa taacgccacg ccgaacgata tttgcgtggc aatacgtagc
     3121 gaagcggcgg atgcggggat cgcgcaggcg attatgcagc agcttgaaga ggcgctaaaa
     3181 caactggcgc aggggtcagg cagcagccag tccttgacgc aggtgcgtcg ctgggacagt
     3241 gcctgtcaga aattatccga tgccaatctg gcgctgattt cagtggctgg cgagtatgcg
     3301 gcggagctgg cgaatcaggc gctggatcgc aacctcaacg tgatgatgtt ctccgataac
     3361 gtcacgctgg aagatgaaat ccaacttaaa acccgcgcgc gagaaaaagg cttgctggtg
     3421 atggggccgg actgcggtac gtcgatgatt gccgccacgc cgctggcttt tgctaacgtg
     3481 atgccggaag gcaacattgg cgtcattggc gcttccggta ccgggattca ggagctgtgc
     3541 tcacagattg cgctggcagg ggagggaatt actcacgcga ttggccttgg cgggcgcgac
     3601 ctcagccgtg aagtgggcgg catcagtgcg ctgacggcgc tggaaatgct cagtgcagac
     3661 gagaaaagcg aagtgctggc atttgtttca aaaccgcctg ccgaagctgt gcgtctgaaa
     3721 attgttaatg ccatgaaagc aaccggcaaa ccgacggtgg cgctgttttt aggttatacc
     3781 ccggcggtgg cccgcgacga gaatgtctgg tttgcctcct cgctggatga ggccgcacgc
     3841 ctggcttgcc tgctttcacg cgtcacggcg cgacgtaacg caatagcgcc tgtcagcagc
     3901 ggatttattt gcggtttgta taccggcggt acgcttgctg ccgaagcggc gggattactt
     3961 gccgggcacc ttggcgtgga agccgacgat acccatcatc acggcatgat gctggacgcc
     4021 gatggtcacc agatcattga tctgggcgat gatttctaca ccgtcgggcg tccccatccg
     4081 atgatcgacc cagccttacg caaccagtta attgccgatc tcggcgctaa accgcaagtg
     4141 cgcgtgttgc tgcttgatgt cgtgattggc ttcggtgcga ccgccgatcc tgctgcctcg
     4201 ctggtgagcg cctggcaaaa agcctgtgcc gcgcgttcag acagccaacc gctgtacgcc
     4261 attgccacgg tgacaggtac cgaacgtgac ccgcaatgcc gctcgcagca aatcgccacg
     4321 ctggaagatg cagggatcgc ggtagtgagt tcgctgccgg aagccacctt gctggcggcg
     4381 gcactgattc gtccgctttc gcctgccact cagcaacaca caccgtcatt actggaaaac
     4441 gtcgccgtga ttaacatcgg attacgcagc tttgcgctgg agctacaaag cgccagcaaa
     4501 ccggttgtgc attaccaatg gtcgccagtc gccggcggca ataaaaaact ggctcgttta
     4561 ttagaacgtt tgcaataagg ggttcccatg tttacatcag tggcgcaagc caatgctgcg
     4621 gttatcgaac aaattcgtcg cgctcgtcca cactggctgg atgtgcaacc ggcttcttca
     4681 cttatcagcg aactaaacga gggcaaaaca ctgcttcacg ccgggccgcc aatgcgctgg
     4741 caggagatga ccggacccat gaaaggggcg tgcgtgggcg catgtctgtt cgaaggctgg
     4801 gcgaaagatg aagcgcaggc gctggcaata ctggagcagg gggaagtgaa cttcattcct
     4861 tgtcaccatg tgaatgccgt cggtccaatg ggcggtatta cttctgccag tatgccgatg
     4921 ctggtggtcg agaacgtgac cgacggcaac cgggcgtact gcaacctcaa cgaaggtatc
     4981 ggcaaagtga tgcgttttgg cgcttacggc gaagatgtcc tgactcgcca tcgctggatg
     5041 cgcgatgtgt taatgccagt attaagcgcg gcgctggggc gcatggagcg cggtatcgat
     5101 ctcacggcga tgatggcgca gggcattacg atgggcgatg agttccatca acgcaatatt
     5161 gcttcctctg cactgttaat gcgtgcgctg gccccacaaa ttgctcgcct cgatcatgat
     5221 aaacagcaca tcgccgaagt gatggatttc ctcagcgtga ccgatcagtt cttcctcaac
     5281 ctcgcgatgg cttactgcaa ggcggcgatg gatgctggcg cgatgatccg cgcaggcagc
     5341 atcgtcacgg caatgacccg caacggcaat atgttcggga ttcgggtaag cgggctgggc
     5401 gaacgctggt ttactgcgcc tgtaaacact ccgcaaggtc tgtttttcac cggcttctcg
     5461 caggagcagg cgaacccgga tatgggcgat agcgcgatta ccgaaacctt tggtatcgga
     5521 ggtgcggcaa tgatcgcagc gcctggcgta acgcgctttg tcggtgcggg tggcatggaa
     5581 gcggccagag cggtatctga agagatggcg gaaatttacc ttgagcgcaa tatgcagttg
     5641 cagatcccag gctgggattt tcagggcgcg tgcctgggac tggacattcg tcgcgtggta
     5701 gaaaccggca ttacgccact catcaatacc ggtatcgccc ataaagaggc ggggatcggg
     5761 cagattggcg caggcactgt gcgggcaccg ctggcgtgtt ttgaacaggc gctggaagca
     5821 ctggctgaaa gcatggggat tggttgagga acgcgcaatg acgatcatcc atcctctgct
     5881 tgccagccgt agtgcaccga attatcgcca gtcctggcgg ttagcgggag tgtggcgacg
     5941 ggcgattaac ctgatgacgg aaagcggcga actgttaacg ctgcatcgtc agggtagtgg
     6001 tttcggcccc ggaggatggg tgcttcgccg cgcgcaattc gatgcgttat gcggtggatt
     6061 atgcggcaat gaacgaccac aggttgtggc tcaagggatt cgcctcgggc gtttcacggt
     6121 taaacagcca cagcgttatt gtttgctgcg tattacgccg ccttcgcatc ctcaaccgct
     6181 tgcagctgca tggatgcaac gcgcggagga aaccggactt ttcggaccac tggcgttggc
     6241 ggcaagcgat ccgctgcccg ctgaattacg ccagtttcgt cactgttttc aggccgcgct
     6301 caatggcgtt aagaccgact ggcggcactg gctgggtaaa ggccccggat taacgccaag
     6361 tcatgatgac acgttgagcg gaatgctgct ggcggcctgg tattatggcg ctttagatgc
     6421 gcgcgccggt cgcccgtttt ttgcctgttc cgacaatctt caactcgtta ccacagcggt
     6481 gagcgtcagt tatttacgtt atgccgcgca aggatatttc gcctcgccac tcctgcactt
     6541 tgttcatgct ctgagttgcc cgaagcgtac cgctgttgcg attgattcgc tgctggcgct
     6601 ggggcatacg tcaggggcag atacactgct ggggttctgg cttggtcaac aattattaca
     6661 aggaaaacca tgaaaacact ggttgtagct cttgggggca acgccttact ccagcgcggt
     6721 gaggcgctga cggcggaaaa tcaatatcgc aatatcgcca gtgctgtacc cgcgctggca
     6781 cgcctggccc gttcttatcg gctggcgatt gttcacggca acgggccgca ggtggggctg
     6841 ctggcattgc agaatctggc gtggaaagag gtagaaccgt atccgctgga tgtgctggtg
     6901 gcagaaagtc aggggatgat tggctatatg ctggcgcaga gtttgagcgc acagccgcag
     6961 atgccgcccg tgacgacggt gctgacgcgc attgaggttt cgcctgatga tccggcgttt
     7021 ttgcagccag agaaatttat tggtccggtt tatcagccag aagaacaaga ggcactggaa
     7081 gcggcttacg gctggcagat gaaacgtgat ggtaaatatt tgcgccgggt ggtggcgtct
     7141 ccgcaaccgc gtaaaattct cgacagcgaa gccatcgagt tgttgctcaa agaggggcat
     7201 gtggtgattt gcagtggcgg cggcggtgtg cctgtgacgg aagatggggc agggagtgaa
     7261 gcggtgattg ataaagatct cgctgctgcg ttgctcgctg agcagattaa cgccgatggg
     7321 ctggtgatcc tcactgatgc tgatgcggta tatgaaaact ggggaacgcc gcagcaacgc
     7381 gccattcgcc atgccacacc ggatgagtta gcgccatttg ccaaagccga tggttcgatg
     7441 gggccgaagg taacggcggt aagtggttat gtcagaagcc gtggtaaacc cgcgtggatt
     7501 ggggcgttat cgcgaattga agagacgctg gcgggcgaag cggggacctg tatttcgctg
     7561 tagtgatgag agcatccggc aatcggtgca ccatgcctga tgcgacgctt gacgcgtctt
     7621 atcaggccta caatgggtac cggagcggta agccggataa ggcgtttacg ccgcatccgg
     7681 caatcaaatg agcacctgct taactgaact tactctgcgc ccacatcacg ccgctggcat
     7741 actccggcgg cagcagcggg atcaaggctt ccagcgtcgc ggtcagacgc gatgtgtcgc
     7801 tgtcggtcaa attcagatgc cccactttac gccccggacg gacttctttg tcgtaccagt
     7861 gcagatgcac cagcggcagt ttcagccagt cataattcac atcgctacca atcaggttga
     7921 tcatcaccga cggactattc actaccggtt gcggtaacgg cagatcggta atcgcccgca
     7981 gatgcagctc aaactggctg atgctggcac cgttttgtgt ccagtgaccg ctgttatgca
     8041 cacgcggtgc cagttcgttg atcagcagac cttgcggggt gacaaaacac tccatcgcca
     8101 tcacgcccac atagcccagc tcctgcataa tcgccgacag catctcttcg gcttgcgctt
     8161 gctgctgcgc attggcctgc ggaaaagcga cgctggtgcg caaaataccg tcctgatgca
     8221 ggttatgcgt cagcggataa aacacggtgc tgccatcaaa tccgcgtgcg ccaaccagcg
     8281 acacttcacc agagaagtta atgccctgct cgacaataca ttcgccgtaa cactctgccg
     8341 gtaactgttc ggtttcattg gcacgtaaac gccattgacc gcgaccgtca tagccaccag
     8401 tgcgacgctt aacaatcgcc agttcaccta aacgctcaaa caccgcaggc cactcgctgc
     8461 gatcggcaag taactgccac ggtgcggtcg gcaggtggag cttatcgaaa agctgcttct
     8521 gagtcagacg gtcggcaata atcgggaaca catcgcggtt cacaaaggcc ggatgacgcg
     8581 ccagctcgcg ggttaatgcg gtttccggcc agcgttcgat ctcagcggta atcacgcttt
     8641 gttgaaaagg caccgccgcc ggttcagcgt ccagcccgac cggccagaca gcaatgccta
     8701 acggttcacc tgcctgacgc agcatacggc ctaactgccc gttaccgagg acgcaaacct
     8761 gtttcatgcc gtacctcgcg ggtccgggtt ttccagcact tcatcagttt gtgctttgcg
     8821 ccagtcattc agacgctggt gtagctcttt atcgtgagtc gcgagaattt gtgctgccag
     8881 taatgccgcg tttgccgcgc cagctttacc aatcgccagc gtacccaccg gaatgccgcg
     8941 cggcatttgt acgatggagt agaggctatc gacaccgctc agtgcggcgc tctgtactgg
     9001 cacgcccagc accggcacca gcgttttggc ggcaatcatg cctggcaggt gcgctgcgcc
     9061 acctgcgccc gcaataatca cctgataacc gttctcttcg gcgctttcgg cgaagctgaa
     9121 cagtttatcg ggggtgcggt gagcagaaac cacttcaacg tggtgcggga cattcaggat
     9181 ttcgaagatt tcggcggcga actgcatggt agcccagtcg cttttggacc ccatcacgat
     9241 ggcgacacgc gccggattat tgcgggaaga catgcgtctt aaaactcctg tggtgcacaa
     9301 ctctcggctt tagagggcac agagaatagc acggaaagag agcaaggaaa acggttgcgt
     9361 ggctgtgaaa tcagcaaagt tgtgggtttt ttaaaacgga aaatgaatca gctcaacgtc
     9421 atccgccgtg actttcacca tcgaaccttc cgtatgccag gcaccgagta ccacgcgaaa
     9481 agcaggttgc tggttggcga taagttcatg taccgccggg cgatgggtat gtccgtggat
     9541 cagccattgc acctgatgtt tttccatcgc actgactacc gcgttttggt taacgtccat
     9601 gatcgccagc gatttactgc tgttggcttc tttactgttc gctcgcattc gcgcggcaat
     9661 gcgtttgcgc acaaacaacg gcagggcgag gaatagcatt tgcagccagg gtttgtggac
     9721 tttggcgcga aaagcctgat aacccgcgtc atcggtgcac agcgtgtcgc catgcataat
     9781 caacacccgg cgaccataaa gttcgagcac cttttcttcc ggcaataatg tcatgccgct
     9841 ttcacgggca aagcgtttgc cgagcagaaa atcacggttg ccatgaatga aataacaggg
     9901 aacgccggaa tcggacaccg ctttgatcgc tgccgccatc tggcgatgga gtgggttggg
     9961 atcgtcgtcg ccaatccatg cttcaaacag atcgccaaga atatacagcg cgtcggcctt
    10021 gcgggcttcc cccgctaaaa aacgcagaaa accggcggtg atcgccggtt cttccacgca
    10081 gagatgaaga tctgcaataa agagtgtcgc cacgattact cgctaacggt cacgctttca
    10141 atgataacgt cttcttttgg cacgtcctgg tgcataccgc tacgaccggt tgcaacacct
    10201 ttgattttgt ctaccacgtc catgccgtca accacttcag caaacacgca gtagccccaa
    10261 ccttgcaggc tttcgccaga gaagttcagg aagtcgttat caaccacgtt gatgaagaac
    10321 tgtgcagttg cagagtgcgg agcctgagta cgtgccattg ccagcgtacc acgggtattt
    10381 ttcaggccgt tgttggcttc gtttttgatc ggttctttgg tggctttttg tttcatgccc
    10441 ggttcaaaac cgccgccctg aatcataaag ccgttgataa cacggtggaa aatggtgttg
    10501 ttgtaaaaac cttcgcggca gtagtccagg aagtttttaa ctgtttcagg tgctttatcg
    10561 tcaaaagttt tgatgacaat atcgccgtga ttggtgtgga aagtaaccat ttttgcatcc
    10621 tgttccgttt gattggtgct tcaacccagt tcgggtcata tatagggtgg tgttatagca
    10681 taaccgcacg atcggatcat cacgcaatgt atgctgattc gcgcgggaaa tatgggtatt
    10741 atacgcaact caattaccca cacatgtcta aacggaatct tcgatgctaa aaatcttcaa
    10801 tactctgaca cgccaaaaag aggaatttaa gcctattcac gccggggaag tcggcatgta
    10861 cgtgtgtgga atcaccgttt acgatctctg tcatatcggt cacgggcgta cctttgttgc
    10921 ttttgacgtg gttgcgcgct atctgcgttt cctcggctat aaactgaagt atgtgcgcaa
    10981 cattaccgat atcgacgaca aaatcatcaa acgcgccaat gaaaatggcg aaagctttgt
    11041 ggcgctggtg gatcgcatga tcgccgaaat gcacaaagat tttgatgctt tgaacattct
    11101 gcgcccggat atggagccgc gcgcgacgca ccatatcgca gaaattattg aactcactga
    11161 acaactgatc gccaaaggtc acgcttatgt ggcggacaac ggcgacgtga tgttcgacgt
    11221 cccgaccgat ccaacttatg gcgtgctgtc gcgtcaggat ctcgaccagc tgcaggctgg
    11281 cgcgcgcgtt gacgtggttg atgacaaacg caacccgatg gacttcgttc tgtggaagat
    11341 gtcgaaagag ggcgaaccga gctggccgtc tccgtggggc gcgggtcgtc ctggctggca
    11401 cattgaatgt tcggccatga actgcaagca gctgggtaac cactttgata tccacggcgg
    11461 cggttcagac ctgatgttcc cgcaccacga gaacgaaatc gcgcagtcca cctgtgccca
    11521 tgatggtcag tatgtgaact actggatgca ctccggaatg gtgatggttg accgcgagaa
    11581 gatgtccaaa tcgctgggta acttctttac cgtgcgcgat gtgctgaaat actacgacgc
    11641 ggaaaccgtg cgttacttcc tgatgtcggg ccactatcgc agccagttga actacagcga
    11701 agagaacctg aagcaggcgc gtgcggcgct ggagcgtctc tacactgcgc tgcgcggcac
    11761 agataaaacc gttgcgcctg ccggtggcga agcgtttgaa gcgcgcttta ttgaagcgat
    11821 ggacgacgat ttcaacaccc cggaagccta ttccgtactg tttgatatgg cgcgtgaagt
    11881 aaaccgtctg aaagcagaag atatggcagc ggcgaatgca atggcatctc acctgcgtaa
    11941 actttccgct gtattgggcc tgctggagca agaaccggaa gcgttcctgc aaagcggcgc
    12001 gcaggcagac gacagcgaag tggctgagat tgaagcgtta attcaacagc gtctggatgc
    12061 ccgtaaagcg aaagactggg cggcggcaga tgcggcgcgt gaccgtctta acgagatggg
    12121 gatcgtgctg gaagatggcc cgcaagggac cacctggcgt cgtaagtaat tgcgctgttg
    12181 ccggatgcga gttttcgcat ccggttatcg cctgcgccac cacaacattc ccataagcac
    12241 catccccggc aaccacaccc acatcaattc agaaattatc acctgatgcc cgtacggcgt
    12301 ggtgtaacga gacaatgcaa acggcgcgac tttaatcacc tgccagggag cgaaaaagcg
    12361 ttcatctgac cacggccaca gccagccaac gcccttaccg ccagtggtta ccgaatccag
    12421 caagctgtgc gatagcaacg agacggttaa aaacagccag cagcgaatca gcccagccct
    12481 gaaccatcgg cgtccaatca gcacacataa cagcgggaca acaaacgcaa acaccagcga
    12541 atgggtaaac ccgcgatgac caaaaacatt gccgtaagca acgccaaatt taaacgacaa
    12601 tacgtcggcg tcgggcagca tcgccagga
//
LOCUS       DAEVZA010000141        12524 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEVZA010000141 DAEVZA010000000
VERSION     DAEVZA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.141"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..563)
                     /locus_tag="LAF92_004118"
     CDS             complement(<1..563)
                     /locus_tag="LAF92_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBI9793041.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIA"
     gene            complement(560..1021)
                     /locus_tag="LAF92_004119"
     CDS             complement(560..1021)
                     /locus_tag="LAF92_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HBI9793042.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            complement(1079..2125)
                     /gene="potD"
                     /locus_tag="LAF92_004120"
     CDS             complement(1079..2125)
                     /gene="potD"
                     /locus_tag="LAF92_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBI9793043.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(2122..2916)
                     /gene="potC"
                     /locus_tag="LAF92_004121"
     CDS             complement(2122..2916)
                     /gene="potC"
                     /locus_tag="LAF92_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBI9793044.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(2913..3770)
                     /gene="potB"
                     /locus_tag="LAF92_004122"
     CDS             complement(2913..3770)
                     /gene="potB"
                     /locus_tag="LAF92_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBI9793045.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(3754..4890)
                     /gene="potA"
                     /locus_tag="LAF92_004123"
     CDS             complement(3754..4890)
                     /gene="potA"
                     /locus_tag="LAF92_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBI9793046.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            5140..6369
                     /gene="pepT"
                     /locus_tag="LAF92_004124"
     CDS             5140..6369
                     /gene="pepT"
                     /locus_tag="LAF92_004124"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBI9793047.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(6515..7636)
                     /gene="roxA"
                     /locus_tag="LAF92_004125"
                     /gene_synonym="ycfD"
     CDS             complement(6515..7636)
                     /gene="roxA"
                     /locus_tag="LAF92_004125"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBI9793048.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(7712..9172)
                     /gene="phoQ"
                     /locus_tag="LAF92_004126"
     CDS             complement(7712..9172)
                     /gene="phoQ"
                     /locus_tag="LAF92_004126"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBI9793049.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTFVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(9172..9843)
                     /gene="phoP"
                     /locus_tag="LAF92_004127"
     CDS             complement(9172..9843)
                     /gene="phoP"
                     /locus_tag="LAF92_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBI9793050.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(10011..11381)
                     /gene="purB"
                     /locus_tag="LAF92_004128"
     CDS             complement(10011..11381)
                     /gene="purB"
                     /locus_tag="LAF92_004128"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBI9793051.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(11385..12026)
                     /gene="hflD"
                     /locus_tag="LAF92_004129"
     CDS             complement(11385..12026)
                     /gene="hflD"
                     /locus_tag="LAF92_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBI9793052.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(12062..>12524)
                     /gene="mnmA"
                     /locus_tag="LAF92_004130"
     CDS             complement(12062..>12524)
                     /gene="mnmA"
                     /locus_tag="LAF92_004130"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBI9793053.1"
                     /translation="AQPGKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPW
                     YVVDKDVENNILVVAQGHEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDI
                     PCTVKALDDDRIEVIFDEPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
BASE COUNT         3104 a         3179 c         3158 g         3083 t
ORIGIN      
        1 gcgataaaac ctaatgtgtt ggtgctgccg gggggagcat tttcatgctg ttgtcctgga
       61 tgcggataat acgtcagtgg cggcgtcggt tgttttaata tctgtgcttg tagaacagcc
      121 aatgcattat tgattccctg ataccagaga tctgcattta aataagccag cgtgtgattt
      181 tctatcagaa ggccgagatg tttatcgtcc agaccacttt caacctggcc gttggtgatc
      241 ataattaacc gtggttcgcg ggtagtaaat agtaacagaa cacttccttt tacattactc
      301 aatgaagatg acgcatgcgc cagcatcgct ttaccatttt gtctgagact ggcttcatta
      361 ttaatgtcgg gtaaaatcac aataaacggt gcaattcccg tttgttggcg aaaaagctcg
      421 gctttacgtg cgatctcttc agcatccggt acagtaccgg taacatccgt aaaaagtgtc
      481 ttcattattg gaatcaatgg tttactggtt gctaccagag gcaggagtat cagtaaactg
      541 agcagtataa tgaatttttt tatgggcggg acttccgttt cctgcgagat tttttggtgg
      601 aggggagcgt gattatcaga ccgacactac cgaaaataat gcctgctatg ccaacaaagc
      661 cccatttaaa aaaattaacc cagggattgt ttatttcggc ttgttctgga tgccctggcg
      721 gataatagac attgactctg tcaccttcgc tatcttcata aaaatcacta ccgatattcg
      781 aattgaaaat tagcgtgtaa tctggcgtag ggcgaaaggc aaccactgga taccaggaac
      841 cattatcatc gacgtcgttt gagtgattgt tgtaccaaac ggcatcaaca atgattcctt
      901 ctgtatgaac cgcgcttttg gtaaaagtaa aatggtaata aaagatgatt gcagaaataa
      961 cgagcaatat cagaccgata ccgagaaaag cgcgggataa aactttactg tcctgactca
     1021 ttagccacat ccttgctaac taaaaaacgg gcggtaatac caccgcccgt ttgctgaatt
     1081 aacgtcctgc tttcagcttc tgataatact cttcataaat gctgctggct gcgccaacgt
     1141 cattctgcca ttcaccattt ttaatggttt cagcatccgg gtagagcgtt ttatcgttcg
     1201 ccacttccgg gcttaacagc ttacgcgccg caaggtttgg cgttggataa ccgatagttt
     1261 cggcaacctg ttttgccaca tccgggcgca gcaggaagtt gatcaatttc agcgcgcctt
     1321 ctttgttttt ggcatttgcc gggatcgcca ggctgtccat ccagaaaatg ccgccttctt
     1381 tcggccacac cacgtcaata ggcgtacccg cctggcgtgc aacgaaagca gaaccgttcc
     1441 agatcatgcc gaggttaact tcgccttcca tgtacgggtt agccgggtta tcggagttaa
     1501 acgctgcgac gtttggcatc agttttttca gctcgttata tgcagcttca atctctttcg
     1561 gatcggtggt gttaccggag tagcccagct tacgcagcgc catctggaac acttcacggg
     1621 catcgtcggt caacagcagg ctacctttgt actcaggctt ccacagatcg gcccagctgg
     1681 taacggattt cggatctacc gcatcaccgt taacgccaat cgccgtcgcc ccccagatat
     1741 acggaatgga gtagtcgtta ttcgggtcaa acggcttgtt gagcatgtct ggatcgaggt
     1801 tgctgaaatt gcttaacttc gacttgtcga tcttctggat catcccttct ttacgcattt
     1861 tatcgacgta ataggttgaa ggaaccacca ggtcataggc accgtctttg tacgttttca
     1921 gcttggcgta catggtttcg ttcgactcgt aagtcgaata gataacctta ataccggttt
     1981 ctttggtgaa ctgttcaagc agtcctggcg gcacgtactc ggtccagttg tagaaataca
     2041 gcgtgttgtt gtcatcggcg tgagcggcgc tcatgcccag tgccagagca cccgccgcga
     2101 gcaggtggcg tgaccatttt ttcatttaac gtcccctgtg ttacctttcg ttttatcacg
     2161 agcaataagc tggctggcaa ttaccatcac cagcgacagc accagtaata tggttgccag
     2221 cgcgttaact tccggcgata cgccgacttt gaccatcgaa taaattttta acggcagaat
     2281 ttcatagctt ggtccggtga cgaacgaaga aactactaca tcgtccatcg acagggtaaa
     2341 gcttaacacc cagcccgccg ccaccgcagg cattgccagt ggcagaatga tttttcgcag
     2401 aatggtaaat tcactggcac cgagatcttt cgccgcttcc agcatccgca cgtcaaaacc
     2461 tttcaggcgc gaatacaccg tcaccaccac aaacggcagg cagaaggtga tatgcgagaa
     2521 cagcagcgac cagaagccaa gctgaatacc cagcagcata aacagcacca gcagagaaat
     2581 cgccatcaca atatctggcg acatcatcac cacaaacagc attccgctaa cgaacggctt
     2641 accgcgaaag cgataacggt acagcgcaac tgccgtcagt gaaccgataa gcgtagcaaa
     2701 cgtcgccgaa aacaccgcca ttgttagtga atgctgcgct gcctgtaaca ggctgtcgtt
     2761 gttcatcagc aggctatacc atttggtggt aaaaccctgc cagttgatgc caaagcgcga
     2821 gctgttaaag gagttcacaa tcaaaataat gattgggata tacaggtacg cgtagatagc
     2881 ggtcataaaa ccgccgcgaa gcagtcgacc gatcattcga gttccacctt tttattcagc
     2941 agacgagaag cgcgccagta aaccagcaac atcaggccca ttacgatagt cagcgtaatg
     3001 ctggtggctg caccaaatgg ccagtcacga atattgagga actgtacctt gatgacgtta
     3061 ccgatcagca ggtttttcgc accgcccatc aggtcggaca catagaacag gcccatcgct
     3121 ggcagcatca ccagcagaca tccggcaata atccccggca tcgtcagtgg gataatgatg
     3181 cggataaagg tctgcaactt gctggcaccg agatcgcgcg ccgcctcaag caaaggctta
     3241 tccagttttt cgatactgga gtacagcggc atcaccataa acggtagcag aatgtaaacc
     3301 agaccgataa tcaccgcact gggcgtgaac atgatacgga ttggtgtgtc gataacgccc
     3361 agccagagca aaaactcgtt gagatagcct ttggtgctga ggaaaatttt cagcccgtag
     3421 atacgaatca atgagttggt ccagaaggga acaatcagca gaaacagcag cagcggacgc
     3481 accttgtgtg gcagcttcgc cagaaaccag gcaaacgggt agcccagcac caggcaggcg
     3541 agggtggcga tcagcgccat attcagcgag tgcaatagca cttcaaaata gagcggatcg
     3601 agcagacgcg tgtagttatc cagcgtaaag accattttga cgaaactggc gtcgtcgcgg
     3661 gtcaaaaagc tggtgccaat gatcatcagg ttgggcagaa agacaaacaa cacaagccaa
     3721 ccgacaatag tgacaatcac tacattctgg aacttacttg tgttcttcat cagccagtac
     3781 gacctcccag ctttctaccc aattaatggc cattttttgg tcgagagagt ggtcaaagtc
     3841 agggtcgtct tcattgaaga attcgctgac catcaccatc ttgccatttt ccagttcaac
     3901 aaccgactcc agcgtcatgc ctttgtagtt acgctcacga acgtaaccaa tcagcccttc
     3961 agcgtggttg tcgtcgttaa tctcttcaac acgtaagtct tccgggcgca gcagaacatg
     4021 cagtttttgc cccggttcaa cggcgaagtt aacgtagata ttacattcgc ggccttcaac
     4081 gttggcgcgt acgcgctgct cgtcgagtcg ctcgatcacc gtggcgttaa acatattaat
     4141 ttcgccaatg aagccggcaa caaacaggtt tttcggctct tcgtagattt cacgcggcgt
     4201 gccgtcttgc tcaatgcgac catcgcgcat caccacaatc ctgtctgaca tggtgagtgc
     4261 ttcttcctgg tcgtgagtca cgaagacgaa tgtaatgcca agcttacgct gtaacgcttt
     4321 cagctcgttc tgcatttgct tgcgcagttt gtaatccagc gctgagagcg actcatccag
     4381 caacaacaga cgaggcttgt taaccaccgc gcgggcaatg gcgacgcgtt gctgttgacc
     4441 accagagagc tgatgcggtt tacgttgagc gaaggtttcc agttgcacca tccgcagggc
     4501 ttccatcacg cggggcgtaa tttcagcagc gggggttttt tgcatgcgca acccaaaggc
     4561 cacattttcg aacacggtca tgtgggggaa aagtgcgtag ctttggaaaa cagtgttcac
     4621 atagcggttt tccgccggaa cgtgggtgat gtcctcgtta tccagcatga tgcgtccgga
     4681 atcaacagtt tccagacctg caatcaggcg aagaacggtt gttttaccgc agccagaagg
     4741 gccaagcagc gtgaggaact cgccattgtt gatagtcaga tccagctggg gaatgacctc
     4801 tttaccatca aagcatttgc gaattcccgc caattgcacc agcggtgaaa gcgaactcgg
     4861 ttgtttattc aattttttac tctgtcccat gtaaacgcaa cggatggctt accgatgcgg
     4921 ggtttgtggt taaccacctt ggtgactctt aatgagggcg gtaattctac ggcaaaccgc
     4981 ttgaatcgcc aatctttgtt gtgaattact ggcttagctt tatattcatt aaggtaatgc
     5041 tgataaatat tcccgcttgc aggggtaaaa gtgacctgac gcaatatttg tcttttcttg
     5101 cttcttaata atgttgtcac aaaaagtgag ggtgactaca tggataaact acttgagcga
     5161 tttttgaact acgtgtctct ggatacccaa tcaaaagcag gggtgagaca ggttcccagc
     5221 acggaaggcc aatggaagtt attgcatctg ctgaaagagc agctcgaaga gatggggctt
     5281 atcaatgtga ccttaagtga gaagggcact ttgatggcga cgttaccggc taacgtccct
     5341 ggcgatatcc cggcgattgg ctttatttct catgtggata cctcaccgga ttgcagcggc
     5401 aaaaatgtga atccgcaaat tgttgaaaac tatcgcggtg gcgatattgc gctgggtatc
     5461 ggcgatgaag ttttatcacc ggttatgttc ccggtgctgc atcagctact gggtcagacg
     5521 ctgattacta ccgatggtaa aaccttgtta ggtgccgatg acaaagccgg tattgcagaa
     5581 atcatgactg cgctggcggt attgcaacag aaaaacattc cgcatggtga tattcgcgtc
     5641 gcctttaccc cggatgaaga agtgggcaaa ggggcgaaac attttgatgt tgatgccttc
     5701 gatgcccgct gggcttacac tgttgacggt ggtggcgtag gtgaactgga gtttgaaaac
     5761 ttcaacgccg catcggtcaa tatcaaaatt gtcggtaaca atgttcatcc gggcacggcg
     5821 aaaggagtga tggtaaatgc gctgtcgctg gcggcacgta ttcatgcgga agttccggcg
     5881 gatgaaagcc cggaaatgac agaaggctat gaaggtttct atcacctggc gagcatgaaa
     5941 ggcaccgttg accgagccga tatgcactac atcatccgtg atttcgaccg taaacagttt
     6001 gaagcgcgta aacgtaaaat gatggagatc gccaaaaaag tgggcaaagg gttacatcct
     6061 gattgctaca ttgaactggt gattgaagac agttactaca atatgcgcga gaaagtggtt
     6121 gagcatccgc atattatcga tatcgcccag caggcgatgc gtgactgcga tattgaaccg
     6181 gaactgaaac cgatccgcgg cggtaccgac ggcgcgcagt tgtcgtttat gggattaccg
     6241 tgcccgaacc tgttcactgg cggttacaac tatcatggta agcatgagtt tgtgactctg
     6301 gaaggtatgg aaaaagcggt gcaggtgatt gtccgtattg ccgagttaac ggcgcaacgg
     6361 aagtcgtgat tgcccgatgc gtatgtggac tgccaattac cgggatgaaa ccgttatatt
     6421 gatactggtc aacatactta tcgtgcctac aggcgttcag tgcatgtagg cacgataagg
     6481 cgcaatcagc gccgcatccg gcttaaatcc aaacttaccc ttcgaagaac caatacccgc
     6541 tattgaccag cgccgcgagc atcgcgagga atgacggatc ttccagcgca tcgccaaaat
     6601 tctccgcagt cagcgcaatg ttgctggcga gtgcatccag tgccggacgg tgcggggaat
     6661 cgatcttctc accattggca tacacgtcgt cgccaatgcg caatacgcgc agaccaccca
     6721 ggcgcaccag cacttcacct tgtttcagcg catcgtagat ttcatctggc tgataaggcg
     6781 gttccggcgg cgcgatatcc agttcatgac gtgactggga tataaactcg ccaaaccatt
     6841 gcttaaagtg ttccggctgg ttgatcaatt cgagcatcat ctcacgcagt ttatccatct
     6901 cttgcggcag aacgtccgca ggatgagcgc gaggtggaac atccggatcg ctgtagtagt
     6961 tgccgcccag ttcacgttgc agcacataat cggcaaatcc gctaattaat tcccgcgtat
     7021 ttggcgcgcg aaaacccacg gaatagttca tcgcattttc cagcgcgtag ccttcatgcg
     7081 ggaatcctgg cggaatataa agaatatcgc caggctccag ctcttcatcg atgatggctt
     7141 cgaacggatc gacctgtaac agatccgggt gcggacagtg ctgtttcatt tgcagctttt
     7201 cgcccactcg ccagcgacga cgaccggtac cctgaatgat aaacacgtcg tactgatcga
     7261 gatgcgggcc gacgccgccg cccggtacag aaaaagagat catcagatca tcaatacgcc
     7321 agtccggtag ttcacggaac ggtcgcatca gcgcggcggt cggctcatgc cagtggttca
     7381 ctgcctgtac cagtaatgac cagttggttt caccgagatg atcgtagctt tcgaacgggc
     7441 cgtggctgac ctgccatttg ccatcctggt gactgaccag tcgactgtca acttcgcttt
     7501 ccatcgccag acccgccaac tcgtctggag agatcgggtc aataaaatta ttaaagccgc
     7561 gttttaacac caccgggcgt ttctgccagt gacgttcaag aaaatcgggc cagttaagag
     7621 tgagttggta ttccatgtta agcatccaca ggctggtatc tgcaaccgat tataacggat
     7681 gcttaacgta atgcgtgaag tatggacata tttattcatc tttcggcgca gaatgctggc
     7741 gaccaaaaat cacctccatc cgcgcaccgc ccagcatgct ctctccggcg acgattttac
     7801 cctcatattg ctcggtgatt tcgcgggcta ccgccagccc tacaccttgc ccagggcgta
     7861 aagtatcaac ccgttgacca cggtcgaaaa tgacctctcg cttgcttaat ggaataccgg
     7921 ggccatcatc ctcgaccaca atatagagat gctcgtcggt ttgccttgca gaaatttcga
     7981 caaactcgag gcaatattta caggcattat ccagcacgtt gcccatcacc tcgacaaaat
     8041 cgttctgctc accgacaaag ctgatctctg gcgaaatatc gagagagata ttgacccctt
     8101 tgcgttgata cactttgttc agcgctgagg tgagattgtc cagcagtggg gcgaccggat
     8161 gcagctcgcg gctgagcaat gtcccgccgc gcatactggc acgatgcagg tagtagccaa
     8221 tttgctgtga aatgcggctg atttgctcca gcattaccgg ctcagcatca ctgacgctca
     8281 tcttttcact acgcagagaa cgcagcgtac tttgcagcac cgccagtggc gttttcagac
     8341 tatgggtcag gtcggtgagc gtcgtacggt atttgtcgta acgttcgcgt tcacttttta
     8401 acaatcggtt caggtttcgt accagactgg tcagttctcg cgttgtggct ggattgagca
     8461 attcgcggtt atgttcttcc agttcgcgga cttcttttgc cagggcttcg atggggcgta
     8521 aactccacca ggcggcgacc cacagcagcg ggatcactaa cagcagattg gctgagagca
     8581 catagataaa ccagctccag accatatagg aactttttag ctccaccgga atggtatcca
     8641 ccaccacaaa ggttaatttt ggcatccgcg atgttgccgg gtagacgttt actgccaccg
     8701 agtgggtcat ctccgcgtcg tcatcatctt cccgcacttc ctgcaactgt tgctgtatcg
     8761 aatgatctcc actcagcaag aggctggtat cgttaacatc cgcttcaatt tcatgaaaac
     8821 cattcgattt cagccagtca ggctggatca tcttcatcag ccagggcacg tcacgttgcg
     8881 cccataaaag ctgcccgttc tcatcataaa ttagcgtcat ggtggggctt tgcttgtcga
     8941 tattttcggg taactcgaca tgcaacttat tgttttccca cttcgcaagg gtatagaaca
     9001 gattgctctc gccacgtaac agccgaaacg tagttttatc gaaactgacg ctataaccga
     9061 tcagcgcgac cattccgtag gcaagcgaaa gcaccagtac taccgcagcc gttgccaaca
     9121 gaaaacgtac ccgcagtgag agcgggaaaa aaagacgcag taattttttc atcagcgcaa
     9181 ttcgaacaaa tagccctggc cgcgaacggt agtaatcact tcttgcggat attgtgcctg
     9241 aatttttttg cgcagacgtc ccatcagtac atcaatggta tggctttccc gcagctccgc
     9301 atccggatag agttggagca ttaacgaatc tttgctcacc actttgccat tattgcgtat
     9361 caacgtttcc ataatggtgt attcgaacgc ggtcagtttg atcacttcgt cattaataga
     9421 taattcacgg cgagagagat caacctgaaa cgggggtagc gaaatgacct gtgaagccag
     9481 gccgctatta cgccgcatta atgcctgcat tcgcgccatc acctcttcaa tatgaaacgg
     9541 tttagtcacg taatcatcag caccggcact taatacttcg actttgtcct gccagctttc
     9601 acgggcggtt aataccagaa tcggcagtga aacatcattg ctacgccagc ggcgaatcag
     9661 tgacagaccg tcctcgtcag gcaatccgag atcgacaatc gcaatatccg gtaaatgttc
     9721 attgagataa taatcggctt ctttggcatc ttctgcgtca tcgacctgat gaccagcatc
     9781 ctgaatctga actttaaggt ggtgacgtaa caacgcattg tcttcaacaa ccagtacgcg
     9841 catttttatt tctccctgtc ttaattatta aaatagtgta acgcgattat gtgtttatgg
     9901 gggtaaacat taaataaacc agcggggagg ggaggtaaag tgaaaaaaga aaaagtggat
     9961 aattttaata aacaggccgg acagcatcgc catccggcac tgatacgagt ttatttcagc
    10021 tcatcaacca tggtgatagc tcgaccaata tagttagccg gcgtcatcgc tttcaggcgg
    10081 gctttctctt cttctggcaa cgccagacca tcgataaact gcttcatgcc ttcggcgtca
    10141 acgcgcttac cgcgagtcag ctctttcagc ttctcgtacg gtttttcgat gccatagcga
    10201 cgcataactg tctggattgg ctcagccagc acttcccagt tgtgatccag ctcatccagc
    10261 agatggtcac ggtttacttc cagtttgctc acgcctttca gggtggattg atacgcaatc
    10321 agcgcataac cgatacccac gccgaggtta cgcagcacgg tggagtcggt caggtcacgc
    10381 tgccagcggg aaaccggcag tttgcttgcc agatgctgca ataccgcgtt ggaaaggccc
    10441 agattccctt cggagttttc gaagtcaatc gggttaactt tatgcggcat ggtggaagaa
    10501 ccaatctcac cagcaatggt tttctgtttg aagtggttaa gggcgatata accccagacg
    10561 tcacggtcaa agtcgatcag aatggtgttg aagcgcgcaa cgcaatcaaa caattcggca
    10621 atgtagtcgt gcggttcgat ctgggtagtg tacgggttcc actgaatacc cagcgaggtg
    10681 acgaactctt cgctgaactg atgccagtca acttccgggt aagcggcgat atgggcgtta
    10741 tagttaccga ccgcgccgtt gattttgccg aggatctcca cctgattaag ctggcggtac
    10801 tggcgctcca tacggtaggc gacgtttgcc atctctttac cgatggttga cggcgtggct
    10861 ggctgaccgt gggtacgaga cagcagcggg atatcgcggt actgaacggc gagatcttta
    10921 atgccatcaa tcagttgacg ccagtacggc aggatcactt catcacgcgc ggttttcagc
    10981 attaatgcgt gggagaggtt attgatatct tccgaagtac aggcaaagtg gatgaattca
    11041 gaaaccgcgt gcagttccgg gatctccgcc actttttctt tcaggaaata ctcaaccgct
    11101 ttaacgtcgt ggttagtggt acgctcgatg gttttgatgc gtgcggcatc ttcttcgctg
    11161 aaattggcga caattgcatc aaggtaaccg attgcgtcgg cagcaaaagc aggaacttcc
    11221 ttgatcgctg cgtgcgcggc cagtttttgc agccaacgta cttcaacttg tacacggaat
    11281 ttcagcaaac catattcgct gaaaatcccg cgcagcgcgc tgactttatc gccgtagcgt
    11341 ccatcgacag gggaaacggc ggtcagtgag gataattcca tagatcacaa ctccggggtt
    11401 aaatgagcaa gaatttgttt tgcctgagtg gtcaggcgat tacgagaaaa catcagttgc
    11461 agacgtccgc cgccgacctg gtgccagagc acggcggcgc gaatgcctgc cagcagggtt
    11521 gcgcgaactt tcgcctgcac ttgtgggctt tgcagtacag caggggaacc ggtgacctga
    11581 atgcgcgggc caagcgggct gatcacatca acatagatag cagccatcgc gctcatcagc
    11641 gtttcggact gtaaatcgaa gtgttcgagc tggcgttgca ggccgttgat ccggttgccc
    11701 agagtgtcga gcgcgccttt cgctgaggag agtttgcgct caagcaccat caagctgagt
    11761 gtgtagcggg ttaattcggc gtttaagccc tggcgactgc tggcattgag cacgccgagc
    11821 agggtttcca gcccgacgcg caggttggct tcgctaccgc caaaaaccgc cagcgtcgag
    11881 ctggggttca tatcaataat actgttgagt gagacgtgta gcgcatcggc atcacaatgc
    11941 ccctggtgag cgagttgttg caccaggcgt gccgactgac aaataccggc cagggcgagg
    12001 gtgatgtcat agtaattctt tgccacgttc actgcttcct tgttttaagt aaagataata
    12061 atcagaccgg cagcggcaga cgctgctcaa taataccgcc accgaggcac acttcaccgt
    12121 tatagaagac ggcagactgg cccggcgtca cggcggcaac cggttcatcg aaaatcactt
    12181 caatgcgatc atcgtccagc gccttgacgg tgcaagggat atcggtctgg cgatagcggg
    12241 tttttaccgt gcaacgcata gtgccggtga atggttcgcg atcgacccag tgcaactgct
    12301 gggcaatcaa cccgacagac atcagccgcg ggtgttcatg gccctgagcg acaaccagaa
    12361 tgttgttttc gacgtctttg tccaccacat accacggttc ttcggtacct tctttggtgc
    12421 caccgatacc cagaccttta cgctgaccga gagtgtgata catcagcccc tggtgctcac
    12481 caatttcatc gccatcgacg gtaatgattt tgcccggttg cgcc
//
LOCUS       DAEVZA010000142        12318 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEVZA010000142 DAEVZA010000000
VERSION     DAEVZA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.142"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..513
                     /locus_tag="LAF92_004131"
     CDS             <1..513
                     /locus_tag="LAF92_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI9793054.1"
                     /translation="ILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARWLGRDLLLK
                     YVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWPYTLISALT
                     TLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKHVDLSASRR
                     SPLTHPKNEG"
     gene            518..1195
                     /gene="ydjY"
                     /locus_tag="LAF92_004132"
     CDS             518..1195
                     /gene="ydjY"
                     /locus_tag="LAF92_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBI9793055.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            1210..1917
                     /locus_tag="LAF92_004133"
     CDS             1210..1917
                     /locus_tag="LAF92_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI9793056.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            1917..2465
                     /locus_tag="LAF92_004134"
     CDS             1917..2465
                     /locus_tag="LAF92_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBI9793057.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            2475..3641
                     /locus_tag="LAF92_004135"
     CDS             2475..3641
                     /locus_tag="LAF92_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI9793058.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            3614..5149
                     /locus_tag="LAF92_004136"
     CDS             3614..5149
                     /locus_tag="LAF92_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9793059.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            5149..5802
                     /locus_tag="LAF92_004137"
     CDS             5149..5802
                     /locus_tag="LAF92_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI9793060.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWFNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            5869..7176
                     /gene="ynjE"
                     /locus_tag="LAF92_004138"
     CDS             5869..7176
                     /gene="ynjE"
                     /locus_tag="LAF92_004138"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HBI9793061.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDIEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(7185..7805)
                     /locus_tag="LAF92_004139"
     CDS             complement(7185..7805)
                     /locus_tag="LAF92_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBI9793062.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            7892..8299
                     /gene="nudG"
                     /locus_tag="LAF92_004140"
     CDS             7892..8299
                     /gene="nudG"
                     /locus_tag="LAF92_004140"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBI9793063.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(8265..8537)
                     /locus_tag="LAF92_004141"
     CDS             complement(8265..8537)
                     /locus_tag="LAF92_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBI9793064.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            8773..10116
                     /gene="gdhA"
                     /locus_tag="LAF92_004142"
     CDS             8773..10116
                     /gene="gdhA"
                     /locus_tag="LAF92_004142"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBI9793065.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(10233..11273)
                     /locus_tag="LAF92_004143"
     CDS             complement(10233..11273)
                     /locus_tag="LAF92_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI9793066.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(11401..>12318)
                     /gene="topB"
                     /locus_tag="LAF92_004144"
     CDS             complement(11401..>12318)
                     /gene="topB"
                     /locus_tag="LAF92_004144"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBI9793067.1"
                     /translation="NAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2868 a         3209 c         3212 g         3029 t
ORIGIN      
        1 atactggtga tcgctggagg aatagttttt ggcccgctct tagggacact actctcatta
       61 attgccgcaa cgctggcctc ctcgtgctca ttcctgctgg cgcgctggct gggacgtgat
      121 ttactgctga aatacgtggg ccatagccat accttccagg ccattgaaaa aggcattgcg
      181 cgtaacggta ttgattttct tattctgacc cgcttaatcc cgttgtttcc ttacaatatt
      241 caaaattacg cttacggatt aaccaccatc gccttctggc cttataccct tatttcggca
      301 ctcacgaccc tgcccggtat tgttatttat accgtgatgg caagcgatct cgccaatgaa
      361 ggcattacgc tgcgctttat tttacaactc tgtctggcgg gcctggcgct gtttattctc
      421 gtccagctcg caaaactcta cgcccgacac aaacatgtgg atctgtctgc ttcgcgccgc
      481 agcccactta ctcacccaaa aaatgaagga tagaacgatg tcgcaacatt attcagtgtc
      541 atggaaaaaa ggactggctg cactctgctt actggctgtt gccgggctta gcggctgcga
      601 tcaaaaagag aatgcggcgg caaaagtgga atacgacgga ctttcgaaca gccaaccgct
      661 gcgcgtcgat gccaataacc atacggtaac catgctggtg caaattaatg gtcgtttcct
      721 caccgacgac actcgtcacg gtattgtgtt taaagatggc tccaacggac ataaatcgct
      781 gtttatggct tatgcgaccc cgaaagcatt ttatgaagcc ctgaaagagg caggtggtac
      841 gccgggcgaa aacatgacga tggataataa agaaacgact catgtcacag gcagcaaact
      901 ggatatttcg gtcaactggc aaggggcggc aaaagcgtat tccttcgatg aagtgattgt
      961 tgacagtaat ggcaagaaac tggacatgcg ctttggcggt aatttaacgg cagcagaaga
     1021 gaagaaaacg ggttgcctgg tgtgtctgga tagctgcccg gtcggcatcg tcagcaatgc
     1081 aacatacact tatggtgcgg tggaaaaacg cggcgaagtg aaattcaaag gcaatgcctc
     1141 agttctcccg gcggataaca cgctggcaac ggttaccttt aaaatcaccg aataaagcca
     1201 ggataaagga tgatgaagat acaatcgcgt aaaatctggt actaccgtat aaccctcatc
     1261 atcctgttgt tcgctatgct gctcgcatgg gcgctgcttc ccggcgtcca tgagtttatc
     1321 aatcgcagcg ttgcggcgtt tgccgccgtg gaccaacagg gtatagaacg ctttattcag
     1381 tcttacggcg cactggcggc agttgtctcg ttcttgttga tgattttaca ggccattgct
     1441 gcaccgctac ctgcgttttt gatcaccttt gccaatgcgt cgctgtttgg cgcgttctgg
     1501 ggcggcttgc tgtcgtggac cagttcgatg gccggcgcgg cgctgtgctt ttttatcgcc
     1561 agagtgatgg gccgcgaagt ggtggaaaaa ttaaccggca aaaccgtgct tgacagtatg
     1621 gacggctttt tcactcgcta cggcaaacac accatcctgg tctgtcggtt attgcctttt
     1681 gtccctttcg atccaatcag ctatgctgcc ggtctgactt caatacgttt tcgctcgttt
     1741 tttatcgcca ccgggcttgg tcaattaccg gcgactattg tttattcctg ggcgggcagc
     1801 atgttaacag gcggtacttt ctggtttgtc accggactgt ttattctgtt tgccctgacc
     1861 gtggtgattt ttatggcgaa gaaaatatgg cttgagcgcc agaagaggaa tgcctgatgg
     1921 gtttaccgcc gcttagcaaa attcctttca ttttacgtcc gcaggcgtgg ctgcatcgtc
     1981 gccattacgg cgaggtgcta agcccgattc gctggtgggg gcggatcccg tttatctttt
     2041 atttggtgtc aatgtttgtt gggtggctgg agcgcaaacg ctcaccgctc gatccggtag
     2101 tgcgatcgct tgtcagcgcg cgcattgcgc aaatgtgcct gtgtgagttt tgcgtggata
     2161 tcaccagtat gaaagtcgcc gagcgtaccg gcagcaccga taaactgctg gcagtggctg
     2221 actggcggca aagcccgctc ttcagcgatg aagaacggct ggcgctggag tacgccgaag
     2281 ccgcaagcgt aacgccgcca acggtcgatg atgccctgcg cacccgactg gctgcgcatt
     2341 ttgacgctca ggcgctcacc gaactgacgg cattgatcgg cctgcaaaac ctgtcggctc
     2401 gttttaattc tgccatggac attcccgctc aggggctgtg ccgtattccg gaaaaacgtt
     2461 cttaaggaga gaagatgcgc cattgtgggt ggttgctggg attgttatcg ctgttttctc
     2521 tggcaacaca tgccagtgac tggcaggaaa ttaaaaatga ggccaaaggg caaaccgtct
     2581 ggtttaacgc ctggggcggc gataccgcaa ttaaccgcta tctcgactgg gttagcggcg
     2641 agatgaaaac ccattacgct ataaacctga agattgttcg tctggcggat gccgcagacg
     2701 cggtgaagcg cattcagacc gaagctgctg ccggacgtaa aacgggcggc tcggtggatc
     2761 tgctctgggt gaacggcgaa aacttccgca ccttaaaaga ggccaattta ttacaaacgg
     2821 gctgggcgga gactctgccc aactggcgct atgtcgacac acagctgccg gtgcgggaag
     2881 atttttcagt gcctacagaa ggggctgaat cgccctgggg cggcgcacaa ctgacgttta
     2941 tcgcccgccg cgatgttacg ccacagccac cacaaacgcc gcaagcctta ctggagtttg
     3001 ctaaagccaa tcccggcacg gttacctatc cgcgcccacc ggactttacc ggcacggcgt
     3061 ttcttgaaca gttgctgatt atgctgacgc ccgatcccgc cgcgttaaaa gttgccccgg
     3121 ataaagccac tttcgcccgc gtcactgcgc ccttgtggca atatcttgac gcgctacatc
     3181 cctatttatg gcgcaaagga aaggatttcc cgccttcgcc cgcgcggatg gatgctctgc
     3241 tgaaagccgg cacattgcgc ctgtcgctga cctttaaccc tgctcatgcc cagcaaaaaa
     3301 tcgccagcgg cgatttgcct gcaagcagtt acagttttgg ttttaaccag gggatgattg
     3361 gcaacgtgca tttcgtcacc attcctgcta acgcaaatgc cagtgctgcg gcgaaggtgg
     3421 ttgccaactt cctgctctca cccaatgcgc aactgcgtaa agcggatccc gctgtctggg
     3481 gcgatccttc tgttctcgat ccgcaaaaac tgcctgatgg gcagcgcgaa acattgcaat
     3541 caagaatgcc gcaggatctg ccgccggtac tggctgaacc gcacgcagga tgggtgaatg
     3601 cactggaaca agaatggtta cgccgttacg gtacgcatta atctttttgc tgtgggcgat
     3661 ggtggcggtg atttatgcac cgctgatccc ggcagctctc acgctgatat cgcctgcgtt
     3721 gtcgttgaca cactggcagg cgttatttgc cgatccacag ttaccgcatg cattactggc
     3781 aacgctggtg tcgacaacca tcgcggcggt cggggcattg ttgattgccc tgctggtgat
     3841 tgtggcgcta tggcctgggc cgaaatggca gcgtatgtgc gcccgtctgc cgtggctgct
     3901 cgccattccc catgtggctt ttgccaccag cgccctgctg ctctttgctg acggagggct
     3961 gctatatgac tatttcccgt atttcactcc gccaatggac cgatttggca tcggtctggg
     4021 cctcaccctt gcagtgaaag aaagcgcatt tctgctgtgg atcttagcgg cagtattgag
     4081 cgaaaaacgg ctgttgcagc aggtcattgt gctggattcg ctgggctaca gccgctggca
     4141 atgcctgaac tggctgctgt tgccctccgt cgcgccttcg ctggcaatgg cgatgctggc
     4201 gattgttgcc tggtcgcttt cggtcgtgga tgtggcgatt attcttgggc caggtaatcc
     4261 accgacgctg gcagtaatta gctggcagtg gttaactcag ggcgacgcgg atcaacaaac
     4321 gaaaggcgca ctcgccagtc tgctgctgat gctgttactc gccgcctacg ttttgctgag
     4381 ctatctgcta tggcgcagct ggcggcgcac tattccccgc gtagatggca ttcgcaagcc
     4441 tgccacacct ttattaccag gcactacgct ggcgagtttt ttacccttaa ccggtgtgct
     4501 gtgtgtggtt ctgctggcga tcctcgcgga tcagtcgacg atcaatagtg aagcactcat
     4561 caacagcctg acaatggggc tggtggcgac attcatcgct ttgctcctgt tactgctgtg
     4621 gctggaatgg gggccacagc gtcgccagtt gtggctctgg ttgcccattc tattgcccgc
     4681 tctgccactg gtggcgggcc agtacacgct ggcgctatgg ctgaacctgg atggaagctg
     4741 gacggcggtg gtctgggggc atctgctgtg ggtaatgccg tggatgctgt ttatcctgca
     4801 acccgcctgg cagcgcattg attcacggtt aattttgatt gcgcaaacac tgggctggtc
     4861 gcgggccaaa atcttctttt acgtgaaatg cccactcatg ttgcgccctg tgctgattgc
     4921 cttcgcggtg ggattttcag tcagtattgc gcagtatatg ccgacgctgt ggctgggcgc
     4981 ggggcgtttt ccgacgctca ccactgaggc ggtggcatta agcagcggcg gcagcaacgg
     5041 tattctcgcc gcccaggctt tatggcaact gttgttaccg cttattattt ttgccctgac
     5101 cgcgttagtc gcaaaatggg taggttatgt cagacaagga ctccgctaat gctctgcgtg
     5161 aaaaatgttt cgctacgttt accagaaagc cgcttgctga caaacgttaa ctttacggtt
     5221 gataaaggtg acattgtcac gttaatgggg ccgtctggct gtggaaaatc cactctgttt
     5281 tcatggatga ttggtgcact ggctggacag ttttcttgta caggtgagct atggttcaat
     5341 gagcaacgga ttgacatgct acccaccgca cagcgtcaga ttggcattct tttccaggat
     5401 gcactgttat ttgaccagtt cagcgtcggg caaaatttac tgctggcgct accgtcgacg
     5461 ctcaagggga ctgccaggcg caatgccgtg aaagacgcgc ttgatcgagc gggactggct
     5521 gaaacttatc accaggaccc tgcgacatta tcaggcggtc agcgagcgcg ggtggccctg
     5581 ctgcgcgccc ttctcgctca gccaaaagcg ttactcctgg atgagccatt cagccgtctt
     5641 gatgtggctc tgcgcgataa ttttcgccag tgggtgttca gcgaagttcg cgaactggcg
     5701 atccccgtcg ttcaggtaac gcacgatctc caggatgttc ctgctgatag ttctgttctg
     5761 gatatggcgc agtggtcaga aaattacaac aaactgcgat aacgcaaagt ttttctcaat
     5821 gcgtcagttc agaatggcgc actcaaaact acaatgtcgg gattttcgat gaaacgtgtt
     5881 tctcaaatga ccgcgctggc aatggcttta gggctggctt gcgcttcttc gtgggccgct
     5941 gaactggcga agcctcttac acttgaccag cttcaacaac aaaatggcaa agcgatagat
     6001 actcgcccca gcgcgtttta taacggctgg ccacaaacct taaatggccc ttctggtcat
     6061 gaacctgccg ccttaaacct ctctgccagc tggctcgaca aaatgagcac cgaacagctc
     6121 aacgcgtgga tcaagcaaca taacctgaaa gccgatgccc cggtggcgct gtacggtaat
     6181 gacaaagatg tcgacgccgt caaaacgcga ctgcaaaaag caggttttac gcatatctcc
     6241 attctgagtg acgcgctaag cgaaccttcc cgtctacaaa aactgccgca ctttgagcag
     6301 ctggtttatc cgcaatggct gcacgacctg caacaaggta aagaggttac ggcgaaacct
     6361 gccggtgact ggaaagtcat tgaagcggcc tggggcgctc ctaagcttta ccttatcagc
     6421 catattcccg gtgctgacta catcgatacc aacgaagtgg aaagcgaacc gctgtggaac
     6481 aaagtttctg atgaacaact aaaagcgatg ctggcaaaac acggcattcg ccatgacacc
     6541 acggtcattc tgtacgggcg tgacgtatac gctggagcgc gtgtggcgca aattatgctc
     6601 tacgcaggcg tgaaagatgt gcgtctgctg gatggcggct ggcaaacctg gtccgacgcg
     6661 ggactgcctg ttgagcgagg aacgccaccg aaagtgaaag cggaaccgga tttcggcgtg
     6721 aagatccctg cacaaccgca gttgatgctc gatatagaac aggcccgtgg actgctgcat
     6781 cgccaggatg catcgctggt gagcattcgt tcgtggccag aatttatcgg tacgaccagc
     6841 ggttacagct atattaaacc aaaaggtgaa atagccggag cacgttgggg acacgctggt
     6901 agcgactcga cgcatatgga agattttcat aacccggatg gcaccatgcg cagcgccgat
     6961 gatattaccg ctatgtggaa agcgtggaat atcaaaccag agcagcaagt ttcattctac
     7021 tgcggcaccg gctggcgcgc gtccgaaacc tttatgtacg cacgcgccat gggttggaag
     7081 aatgtttccg tgtatgacgg cggctggtac gaatggagca gcgatccaaa aaatccggta
     7141 gcaaccggtg aacgcggccc ggacagtagc aaataacatt gcaattactg acgctggtgc
     7201 gacttcagcg tcagataccc actccacacc cgcgtaaagg tcgtcatcca gcacaacgca
     7261 ccgaagatcc acgcaaacca cgcaaaccat gcagggaata aacatcccag cacaaacagt
     7321 aagatcgttt ctgtgccttc ggttaacccg cccagataat aaaacgattt atgcgcataa
     7381 ccgggattat caatctgatg tttcgccgcc agcgcagcaa aagcgagaaa actgctgcct
     7441 gtgccgataa acgcaaacag cagccagccg cctgccaggg cgttttgctg cggtgcggcg
     7501 aggataaacc caaaaggtac cagcgcgtaa aaaagaaaat cgagagaaat atcaagaaag
     7561 ccgcccgcgt cggtaagccc tctgcgccgc gccagcgcac cgtccagacc atcaagcagc
     7621 ctgttcaaca aaatgacgac aagcgccgcc agataccagc ccagcgccag aaaaggcagt
     7681 gccagcacgc cgatggcaaa tcccactaat gttaaaccat ctggcgtaat gcccggttta
     7741 tcaagaaccc gcacgcactg atgcaacaac ggtttaatcc ggggatgaag atggcggtct
     7801 agcacaggca ctccttaaat ataaagcctt tctgattgag caacagtgcg gatattatgg
     7861 catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg attgaagttg ttgccgccat
     7921 cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc gcccagagcg atcaggcggg
     7981 attatgggag tttgccggtg gtaaagtcga gccggatgaa agccagcggc aggcgctggt
     8041 gcgtgagtta cgcgaagaac tgggcatcga agcaactgtg ggtgagtatg ttaccagcca
     8101 tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc tggcacgtac ccgacttcca
     8161 cgggacgtta caggcacatg aacatcaggc gctggtctgg tgctcacctg aagaggcgct
     8221 gcaatatccg ctggcccctg ctgacattcc attattagag gcgtttatgg ctttacgcgc
     8281 cgccagacca gcggattagt gctaagggtt ttgtcatccc gctggcattg cagcagtatt
     8341 ccctcggctt taattaccgc gccttcagaa taatttttat cctgataaac gcagcactga
     8401 gtacagggct gcgctgactg cccgcctgaa ctgaacactt ctggcggtac gtttacctcc
     8461 acgtccggac gataatgcgg gttagccagt gcaattaatg gaaatgctaa tactacggcg
     8521 aacaatgctc gactcacagg gaactcctta acgttattgt ctctgctact gataacggta
     8581 gccgggtggc aaaactttag cgtctgagtt atcgcatttg gttatgagat tactctcgtt
     8641 attaatttgc tttcctgggt catttttttc ttgcttaccg tcacattctt gatggtatag
     8701 tcgaaaactg caaaagcaca tcacataaac aacataagca caatcgtatt aatatataag
     8761 ggttttatat ctatggatca gacatattct ctggagtcat tcctcaacca tgtccaaaag
     8821 cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg aagtaatgac cacactctgg
     8881 ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat tactggagcg tctggttgaa
     8941 ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg atcgcaacca ggtacaggtc
     9001 aaccgtgcat ggcgtgtgca gttcagctca gccatcggcc cgtacaaagg cggtatgcgc
     9061 ttccatccgt cagttaacct ctccattctc aaattcctcg gcttcgaaca aaccttcaaa
     9121 aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg gtagcgattt tgacccgaaa
     9181 ggtaaaagcg aaggcgaagt gatgcgtttc tgccaggcgc tgatgaccga gctttatcgt
     9241 catctgggcg cggataccga cgttccggca ggtgatatcg gcgttggtgg tcgtgaagtc
     9301 ggctttatgg cggggatgat gaaaaagctc tccaacaata ccgcctgcgt cttcaccggt
     9361 aagggccttt catttggcgg tagtcttatt cgcccggaag ctaccggcta cggtctggtt
     9421 tatttcacag aagcaatgtt aaaacgccac ggtatgggtt ttgaagggat gcgcgtttcc
     9481 gtttctggct ccggcaacgt cgcccagtac gctatcgaaa aagcgatgga atttggtgct
     9541 cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg atgaaagcgg attcacgaaa
     9601 gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg atggtcgagt ggcagattac
     9661 gccaaagaat ttggtctggt ctatctcgaa ggccaacagc cgtggtctgt accggttgat
     9721 atcgccctgc cttgcgccac ccagaacgaa ctggatgttg acgccgcgca tcagcttatc
     9781 gccaatggcg ttaaagccgt cgccgaaggg gcaaatatgc cgaccaccat cgaagcgact
     9841 gaactgttcc agcaggcagg cgtactgttt gcaccgggta aagcggctaa tgctggcggt
     9901 gtagcaacgt caggccttga aatggcgcaa aacgctgcgc gcctgggctg gaaagccgag
     9961 aaagttgacg cacgtttgca tcacatcatg ctggatatcc accatgcctg tgttgagcat
    10021 ggtggtgaag gtgagcaaac caactacgtg cagggcgcga acattgccgg ttttgtgaag
    10081 gttgccgatg cgatgctggc gcagggtgtg atttaagttg taaatgcctg atggcgctac
    10141 gcttatcagg cctacaaatg ggcacaattc attgcagtta cgctctaatg taggccgggc
    10201 aagcgcagcg cccccggcaa aatttcaggc gtttatgagt atttaacgga tgatgctccc
    10261 cagggaacat ttcttatggg ccaacggcat ttcttactgt agtgctccca aaactgcttg
    10321 tcgtaacgat aacacgcttc aagttcagca tccgttaact ttctgcgata gcagcagata
    10381 tgccagtaaa gaaatcccat tagactattt ttttgataat cttcttcgct ttcgaacaac
    10441 tcgtgcgcct ttcgagaagc aagcattata taatgccagg ccagttcttc ttcaattgtc
    10501 ccgttttgaa aagctgtgct tgatatcgag atcatccatg ataattccgc cgcccatatt
    10561 agcttcgccg aggatttacc ggagcgatta ttagcgcaat cagagatata gtctgaggga
    10621 aaaacagcaa atttattcaa caaggcgata acctgctctg gggcttcctc catgtttgct
    10681 ttaaaggtat tggctccatg gtcgccagaa agaaaatgct ccattaaggc acaataactt
    10741 tcgctatctt cgatacccca ttgatcctct aaagactcgc gtcttttact tatgatatcg
    10801 atcgagtcaa aaggaagcac atgatattgg aaggtatctt tgccaggttc aggctttcgc
    10861 ggccagaact ccagcgtttc agaccattgc ttatgataga atcgataagg tgcgatcaat
    10921 tgtagcgcct gtaacttctc gatactgagc ggctcaatac ctttagcctg ataataatgc
    10981 agttgttctt tttttgcttt aaaaccggcc cgaacaataa gccccatcat aattaataga
    11041 taaagaaaag agcatcccgc ggtaatcagg cctctttcat tcaaaccgtt ggatgttatc
    11101 gctgcgaata caaacattac agcgacaaca catgttaaat aaaaccccca cttacaaagc
    11161 agcatggcct tattttcttt aatcatccgt tcaaaattac tattaaatat ttcccagcca
    11221 ttaaaagaat acttctcgct cccaggatgg ttttgtaata aaactttttt catcacattc
    11281 cctgttatta catactttag aggcgataat tttatccatg caaaaaaaat atccaacgaa
    11341 ttttcttgat ccgctggata tttcagaata tgactcgaat agcacgaaag attcactcgc
    11401 ttacgctatc gccccgcttc cgacttcatc tgctggcgga ctttttttcg cactacgttt
    11461 acgcggtgca gcctttttct tatcagcact gccaccactg cccggagcca caatgccgcg
    11521 aaactgccgc accggcgtac gtttggcttg atcaataagc tgatatagcg tccccaccag
    11581 cggctgcata aagtcctgat agcgacactg cttttcgctg atttgcgtca gcaccgattc
    11641 ccagtgcgcg gtcatgtccg gtcgcgtcgc catttctggc agcgaatgga ataacgcttt
    11701 tccggcgtcg gtagagtgga tatagcgccc ttttttggtc agaaaaccac gcttgaacag
    11761 cagttcaata atcccggcac gggttgcttc tgtccccaga ccatcggtcg cacgaaggat
    11821 ctttttcaga tctttgtcct gcacaaagcg cgcgatcccg gtcatcgccg aaagcagtgt
    11881 tgcatcggta aaatggcgcg gcggctgggt ttgccgctct accacttcac ctttttcaca
    11941 cagcaactca tcgcctttcg ccaccacagg caatggcgta ccgtcgtttt cttcatcgcg
    12001 ctctttgctg cctaacagcg tgcgccagcc tgcttcagca agaaaacggg ctttagcgac
    12061 aaatttgcct ttggcaatgt ccagttcgat aacacacttg cggaacaccg catccgggca
    12121 gaactgcatc aggtattgac gggcaatcag gttatagacc ttcgcttcgt tctccgtcag
    12181 gttgatcgca gaactccgtg cggtcgggat gatggcgtgg tgcgcatcga cctttttgtc
    12241 atcccaacag cggttgcgta tatctggatc taccactggc tgcggcaaca gatctggtgc
    12301 atgaacgctg atggcatt
//
LOCUS       DAEVZA010000143        12221 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEVZA010000143 DAEVZA010000000
VERSION     DAEVZA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.143"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1069)
                     /gene="nepI"
                     /locus_tag="LAF92_004145"
     CDS             complement(<1..1069)
                     /gene="nepI"
                     /locus_tag="LAF92_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBI9793068.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCG"
     gene            complement(1280..1732)
                     /locus_tag="LAF92_004146"
     CDS             complement(1280..1732)
                     /locus_tag="LAF92_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBI9793069.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDCSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(1785..3119)
                     /gene="adeQ"
                     /locus_tag="LAF92_004147"
                     /gene_synonym="yicO"
     CDS             complement(1785..3119)
                     /gene="adeQ"
                     /locus_tag="LAF92_004147"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBI9793070.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3294..5060
                     /gene="adeD"
                     /locus_tag="LAF92_004148"
     CDS             3294..5060
                     /gene="adeD"
                     /locus_tag="LAF92_004148"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBI9793071.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5105..6496)
                     /gene="uhpT"
                     /locus_tag="LAF92_004149"
     CDS             complement(5105..6496)
                     /gene="uhpT"
                     /locus_tag="LAF92_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBI9793072.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(6634..7953)
                     /gene="uhpC"
                     /locus_tag="LAF92_004150"
     CDS             complement(6634..7953)
                     /gene="uhpC"
                     /locus_tag="LAF92_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBI9793073.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(7963..9465)
                     /gene="uhpB"
                     /locus_tag="LAF92_004151"
     CDS             complement(7963..9465)
                     /gene="uhpB"
                     /locus_tag="LAF92_004151"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBI9793074.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(9465..10055)
                     /gene="uhpA"
                     /locus_tag="LAF92_004152"
     CDS             complement(9465..10055)
                     /gene="uhpA"
                     /locus_tag="LAF92_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBI9793075.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10130..10420)
                     /gene="ilvN"
                     /locus_tag="LAF92_004153"
     CDS             complement(10130..10420)
                     /gene="ilvN"
                     /locus_tag="LAF92_004153"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBI9793076.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10424..12112)
                     /gene="ilvB"
                     /locus_tag="LAF92_004154"
     CDS             complement(10424..12112)
                     /gene="ilvB"
                     /locus_tag="LAF92_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBI9793077.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
BASE COUNT         3164 a         3479 c         3077 g         2501 t
ORIGIN      
        1 cgccacaggt attagcaagc tgaataaccg ccacctgaat agacccggct ttttctgcct
       61 gatcggccag cgagcgggtg atccacgttg accagccgac gggaaccaat gcaaaagtta
      121 gcccccagat aatcgccacg ccggtagcaa cgattttatc gcttccccac aacgtcagca
      181 ccaacgcact cacagccagt attaacggcg cgcctgctaa ggccagtttt accgaacgtt
      241 taagaatgaa tgacgaaagc gacgtaccga taaagctggc aatgccaaaa ctcaacagca
      301 ccagcgttaa gccatccacg ccgaatcccg ccaggttcat atacaccggg cgaatatatg
      361 tgaagaaagc aaactgcccg gcgaaagaca tgaagatggc gatcatccct gccatcacac
      421 ccggacgttg taataagcgg aaagtatttt gtttctgatg cgagggttcg cctggcagtg
      481 aaggcaatga tttgataatc cagaaaatac acagcacgcc catcaccgcc gccgcattaa
      541 agacattgcg ccaaccgata agctcgccta aaaaactgcc caacggcgcg gcaatcacca
      601 gcgcaataga aaccgcgccg aagatcaccg acagcgcctt cggcaccgta cgcggcggca
      661 ccagacgcat ggtcagcgac gccgacatcg cccagaaccc gcccagcgcc agccccagac
      721 aggcacgacc gattaaaagc aaactgaatg agttagcaaa ggaaaccagc aagcaggaga
      781 gcgtcagcaa aacggcaaac aaaataacaa cgtagcggcg gtcagtagcc tgaattgtct
      841 gggtaataaa caaactggca aacattgcca caaaggcggt cacggtcacc gattgcccgg
      901 caaccccttc cgaaatgcct aaatcctggg ccattggcgt caacaaactg acgggcaaaa
      961 actcaacgat aatcagacag gcgacacaaa acgccaccga gaaaacggct gaccagttcg
     1021 gtcgggtgat ggcatccgcg ccgcggtttt cggcaataaa ttcactcatg gtgttacccg
     1081 tggcatgttt caggggaaaa gccgaccact ttaacattga aatgtgtgac gcatttaacg
     1141 tttttgcaac ttttacggtg ttgcttgagc tggagttaag atcgaaacgg aacagggcaa
     1201 agattaccgg atgcggcgtg aacgtcttat ccacccgaca ggtcaggcgc ggtctgtagg
     1261 ctgataagac gaaacggttt caatgcaacc agtgaacgcc ctcggcaggc tgaaagaacg
     1321 cgttatagcg catctgaaaa gcattaatat cctgcatttc aggttcggtt tcgcgcagaa
     1381 aacctagcgc cagcctgacc tgagcgtcgg taatcggcgc ggcgaggcga gctttttgta
     1441 aaatccgatg ccattgcagc aggttctgtt cgctacaatc gacaatgaca atttgccaga
     1501 tgagcaacac ctgcgcggcg ttttgcaggt gcgactcatc cggacgatgc aaataacgta
     1561 aaaattcgtc accagctgac tttcccattt gatcgaccat cgactccacg ggtacgacat
     1621 cgatgttcag ccgatcgctg agacggcgaa tcgcttttct ggccccggat gtcagcaccc
     1681 gaaaaccaac cacaaacact accgccagcg tggcgagcat tatccagatc atgagttctc
     1741 ctgcgtaatc agaactcatg atatctggaa accctcgcca gttactaatc caccagaata
     1801 atcttcagtg caaacagagc tgcgaccacc accacacaca ggttcagatc gcgccagcgc
     1861 ccggtgcaca ctttcatgat gcagtacgac ataaagccga gtgcaatccc ttcggtgatc
     1921 gagaaagtaa agggcatcat caccgtggta ataaacgcag gcaccgattc ggtaaaatca
     1981 tcccagttaa cgcgcgccag gctcgaagtc atcagcacgc caacaaagat taacgctccg
     2041 gcggttgcgt aaggaggaac catcgccacc agcggtgaga agaacataac caacaggaac
     2101 ataacgccaa ccacaaccgc agtcagcccc gtgcggccac cgactgccac accagaagta
     2161 ctttcaatat aggcagtaac agacgaggtg ccgataaacg cacccgccac cgaactgacg
     2221 ctatcaacat acagcgcctt attcatattg gggaatttac cgttaccatc tattaagccc
     2281 gctttatcag ttacaccaat taatgttcct gatgaatcaa ataggttgat cagcataaag
     2341 gagaaaatga taccggcgag ttcaagtgtt aacgcgccgc tcaaatctac ttcaccaatg
     2401 acgccgctaa tatcaggcgg aatggaatag acgccgctaa aatgaacatc accgaaaaat
     2461 aatccacagc aagacgtcac cacaatagaa accagcaccg cggcatgaaa atgacgtgat
     2521 gacaacacgg ttataataaa aaaccctaaa atacctaaca acacgccgtg agaacttaaa
     2581 tcgccaatca tcaccagcgt gtctttattg gcgacaataa cgccagtatt ttttaatccc
     2641 attaaggcga taaataatcc aattccgctg gtgataccaa tacgtaaact taagggaatg
     2701 ttggagatca tccagtaccg gatacgaaac agcgtgagca aaaatagtcc aactgcgccc
     2761 cagaatattg cgcccatccc ggtctgccag gagatgccca tcgcccccac gaccacgaag
     2821 gcaaagaagg cgttcagccc cattgccgga gccagcgcca cgggtaagtt agcaaatatc
     2881 cccatcgcaa tactgccgat accggcaatc aaacaggtgg taacaaacac cactttcggg
     2941 tccatttgtg ccgcgccgag gatttgcggg ttcacaaaaa cgatgtacac catggttaaa
     3001 aaagtggtca tccccgcaat caattctgtg cggacggtgg tcccatgctg aggtagttta
     3061 aataatcgcg aaagcgtccc tgattcatta ctcacgtaat cggtattgtc attattcatt
     3121 tttttgtcca ttggaggaga tttaatccct tgcttttgta aaacaaatga caacaacggt
     3181 tcagtgataa ttatcacatt tcaattgcac attaatggat attctttaat aatctcgcga
     3241 cgtttcttta tgataaataa taatcaaatt gataaaatca aaatgagaaa aatatgaata
     3301 attctattaa ccataaattt catcacatta gccgggctga ataccaggaa ttgttagccg
     3361 tttcccgtgg cgacgctgtt gccgattata ttattgataa tgtctctatt ctcgacctga
     3421 tcaatggcgg agaaatttcc ggcccaattg tgattaaagg acgttacatt gccggtgttg
     3481 gcgcagaata cgctgatgct ccggctttgc agcggattga tgctcgcggc gcaacggcgg
     3541 tgccagggtt tattgatgct cacctgcata ttgaatccag catgatgacg ccggtcactt
     3601 ttgaaaccgc taccctgccg cgcggcctga cgaccgttat ttgcgaccct catgaaatcg
     3661 tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg ctgtgccgaa caggcaaggc
     3721 aaaaccagta cttacaggtc agctcttgcg tacccgccct ggaaggctgc gatgttaacg
     3781 gtgccagttt tacccttgaa cagatgctcg cctggcggga ccatccgcag gttaccggcc
     3841 ttgcagaaat gatggactac cctggcgtaa ttagcgggca gaatgcgctg ctcgataaac
     3901 tggatgcatt tcgccacctg acgctggacg gtcactgccc gggtttgggt ggtaaagaac
     3961 ttaacgccta tattactgcg ggtattgaaa actgccacga aagttatcag ctggaagaag
     4021 gacgccggaa attacaactc ggcatgtcgt tgatgatccg cgaagggtcc gctgcccgca
     4081 atctcaacgc gctggcaccg ttgatcaacg aatttaacag cccgcaatgc atgctctgta
     4141 ccgatgaccg taacccgtgg gagatcgccc atgaaggaca catcgatgcc ttaattcgcc
     4201 gcctgatcga acaacacaat gtgccgctgc atgtggcata tcgcgtcgcc agctggtcga
     4261 cggcgcgcca ctttggtctg aatcacctcg gcttactggc acccggcaag caggccgata
     4321 tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca ggtactggtg aaaggcgagc
     4381 cgattgatgc gcaaacctta caggcggaag agtcggcgag actggcacaa tccgctccgc
     4441 catatggcaa caccattgcc cgccagccag tttccgccag cgactttgcc ctgcaattta
     4501 cgcccggaaa acgctatcgg gtcattgacg tcatccataa cgaattgatt acgcactccc
     4561 actccagcgt ctacagcgaa aatggttttg atcgcgatga tgtgagcttt attgccgtac
     4621 ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt gcttggcggc tttggactga
     4681 atgaaggtgc gctggctgcg acggtcagcc atgacagcca taatattgtg gtgatcggtc
     4741 gcagtgccga agagatggcg ctggcggtca atcaggtgat tcaggatggc ggcgggctgt
     4801 gcgtggtacg taacggccag gtccaaagtc atctgccgtt gcccattgcc ggcctgatga
     4861 gcaccgacac ggcgcagttg ctggcggaac aaattgacgc cttgaaagcc gccgcccgtg
     4921 aatgcggtcc gttacccgat gagccgttta ttcagatggc gtttctttct ctgccagtga
     4981 tccccgcgct aaaactaacc agtcaggggc tatttgatgg cgagaagttt gccttcacta
     5041 cgctggaagt cacggaataa taaaaaagcc cggcgtcatg ccgggcaaaa gtcaccagtt
     5101 acgtttatgc cactgtcaac tgctgaattt ttttctcgcg gcggattttg cgttcttcca
     5161 tcaccgccac tatcgccatc aggcagatac aaccaatcgc ggcgatatca agtgcggcga
     5221 aggtgcctgc ccagccggta aggccgaata ccggcgtccc atcggcaatc attcccagac
     5281 ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt gcctttaata ccatcggcag
     5341 cgccaatcgc ttttttaggt acaaagccaa cagcagccac accaatcaac aattgcgggc
     5401 caaagaccag gaaacccaac gcaaagagag aagccagata gatatattcg ttactggcat
     5461 gttgatacac accgagcgtg gcgataatca gcgccagcgc gatgcaggcc accaggccac
     5521 ggcgaccgtt cgccaggtca gagagccagc cccacagcag cgtaccgacc agcgcaccag
     5581 cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga gagtttcagt tcctggaacg
     5641 cgtatacggt tgaccactgg tcgataccaa tacgtaccac atagaggaaa atgttggcga
     5701 agcacagcag ccagatcact ttgtttttca gcacatactc aacaaagatc tgccacttgg
     5761 tcatatcggt agattctgtc tctttgtcct cttcgctgat ctcctcgccg aacagttctt
     5821 cagctttgcc gaggccataa gattccgggg agtcgctgcc gtaacgcagg ccgataaaac
     5881 cgacaatcag cgcgataatc gacgggaaga taaacatgcc gatcacgtgg ccatcgaaca
     5941 ggtaatttgc cccgaacagc gccacacctg ctgcgcctgc accgccaagg ttgtgagaaa
     6001 tattccagaa accgaggaat gtcccgcgtt tacgacgcgg cgtccatttg gtgatggtgg
     6061 agtaactgca cgaaccgccg gtactctgga aaaagccgct taaggcgtag aaggcaatca
     6121 tcaggaacag gctaaccgag ccgctgccca tactggcact gaagcccagc atacaaatgg
     6181 cagacaggat aagcataaac ggcaggaatt gtttggtgtt tttgccgtcg gcgtagtagg
     6241 aaaccagcgt tttacccacg ccataagtga tggagaaacc caggccgatc atccccagct
     6301 gcgtcatgct caacccgtag gtcgaaatca tgtcgttctg cgcgatgtta aagttcttgc
     6361 gaatcaggta catcgtcagg tagccgataa agaccaccag gtaggattgc atgaacggtt
     6421 tgaaccacat tttgcgccgc acttcgagcg gaaggtccag ggtcggcttg cgaacctggt
     6481 ttaagaaagc cagcatgggt tactcctgaa atgaatacct gcgtgaggca tgcattgtaa
     6541 aaatcagcag caaatcaggc ctgagatagc gtccaggcaa aacctggaaa atttcttagt
     6601 tttgccggat atgaagtgaa aaggtgagat gcatcacgct tcgcgcggtg tctgggcgtt
     6661 caaaaagggc agtaacagca gtgcggaaat cccggcggcg atagagataa ccacaaaaaa
     6721 tccgctccag tgccaggtat cgagtacttt cgccagcggc caaccagcaa gcgacgcccc
     6781 cagataagca aacaagccga caaaccccgt cgccgcccct gccgcctctt tgtgggaaca
     6841 ctctgccgcc gccataccga ttaacatctg tgggccaaag acaaaaaaac caatggtgaa
     6901 gaagcaggtt gcctgcatca cgtagctggc aaatggcatc agccacaggg agccgactga
     6961 aagcaaaatt ccggcggcga aaatcaaatt catcggccct cggttgccgt taaacaattt
     7021 gtccgagccc caacccgcta ccagcgcacc gataaatcca cccagttcaa acatcgtcac
     7081 tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg gacatataca aattgcccca
     7141 gtcgttgatc gccgcccgga ccacatagac cagcacatag caaaacgaaa gcagccagat
     7201 atacggattc agcaacacat atttggtgag gatctcttta cgcgtcagcc ctgccccttc
     7261 ttgttgttga gctatctcca gcgcgtcgtg tcgccattca ccgaccgccg gtaaacctaa
     7321 cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt cccacgacga tcgccataca
     7381 accagcaatc atcatcccgg cacgccagcc gtaatgtagt gcagccgctg ccatcacaat
     7441 gggaatgagt gcgccgccga cgttatgcgc cgtgttccat aatgcccacc aaccgccgcg
     7501 ctcggtacgt gaataccagg ccgttaacag acgcgcacac accggtgaac cccagccctg
     7561 gaaaaaggcg ttcagcaccc agagcacggc aaacgcccat agcgacgtcg agaagccaaa
     7621 cagaatgttc atgatgcccg tggcgataag ccctatcccc ataaaataac gggcatttga
     7681 gcgatcgctg acaatgccgg agacaaactt cgacacgccg taagtaatgt aaaacagggt
     7741 cgctaacagg ccgatatcgc tacggctgag cacgccgtta gcaaggattt ctggtaccgc
     7801 ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa cccagccaga tggtcagcag
     7861 aatatgccga cgccagtagc gatagcgggc atcaatttca tatttatcag tcattaatgg
     7921 cgcatcggca ggcgctttca gaaacggcaa catcatcaaa ccttagacat agcgttgagg
     7981 tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg tgtaatgtgc cacccagcgc
     8041 cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt tgcccggaac ccggcggcaa
     8101 accgctgcca tcgtcttcaa taaccagcat caaccgttca tcctgctgcc agccttgcag
     8161 ggtgaccgcg ctggcatcag catgtttcac aatgttgttc agcccttcct ggcagacacg
     8221 aaacagcgtc acgcgctggt tttcgcttaa cgctgattca tcgattcgcc attcgagatg
     8281 gctgacaata ccgcgccctt ccagctccat ttcccgcatc agtgagcgga tggcctgctc
     8341 cagggtgaga tcatccaact ggcgcggacg taaccgaccc aacaaacggc gcaccgcgtc
     8401 gtaaacgccc agcgatagtt gttcgatgag ctgcccgctc tgcttcacgc tggcgttatc
     8461 tgccgccagc cgctgaacaa tgcccgcctg agtacgaata gcagtgatgg tctgaccgat
     8521 atcatcatgc agctcacgcg ccacatcacg acgcacgctc tcttcggttt ccagcaaccg
     8581 ttcagccaga tgctgattgc gcgccagttc cttttgcagc gactggttaa gttcacgcaa
     8641 ccgctggatg ccagcgccca gtaacaaccc tgtcagactt tgcaccagca acgagagcaa
     8701 taaatccacc ggatgatcgc gccaggtttg actggcgatc agcgcgatgg cgttcatcaa
     8761 cgtcgcgatc agcgcccctt gccaaccgta gtgccaggcc agcgcgataa tcggcagcgc
     8821 caggcagaat ggcgtaaagc gcgacagttc gtccggcaat cccaactgga gccagagact
     8881 aataacaaac aacagcaagt accagaccag atgccgcccg cgccagttga tcggttgaga
     8941 aaccagtgac gggccgagcg gcagccaggt gttattggcg agatagtgcc agaacaccag
     9001 acatatcggg gccagcgtca ggccgccagt taaagtcagc aacagcgcat tccacgactc
     9061 tttgccgtgc caaagccagg gcagcgactg caacaacgcc gccgccgtta acgccgcacc
     9121 ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag cgcgagatca gcgccacggg
     9181 cagtaacgtc agtaaactac cgatcatcaa taacggaaaa tgggttaaac cgaccgcctg
     9241 cgttagccag taaatcagca gccactccgc gcccagcagc accggccagt atccgcgcgg
     9301 gcattgcagc attagcccca gacgcagacc aaacggaaat aacagcaccg ccatatcagg
     9361 gcgctcaacc aggtgcaggc tgatactcca caggcaaaac catgcggcag agaagataaa
     9421 aaagcaggca ataacggtaa ttaagcggga gaacaacgtc ttcatcacca gccatcaaac
     9481 atgcggcgcg ccagctctac gtcgttactg acacccagtt tttccatcag gttggcgcga
     9541 tggacgtgca ccgttttcgg cgacaagccc agttcggcag caatctcttt caccgccatt
     9601 ccttgcgcca gtttttccgc cacctgccgt tcgcgtttgg ttagcgggtc ctgacgaccg
     9661 gatgccagtt taatggcaat atccggcgtc agataacagc cgcccgtggc aaccgtatgc
     9721 accgccgcaa tcagttcgtc agggctacag cgtttggaga gaaagccgcg tgcccccgcg
     9781 ttaagcgcct gctcaaccag cgccggactg tcatgaacag agagcattat tgtcgccata
     9841 cctttcggca gctggcttag cagttccaga ccggagatat cgggcatgga gatatcgcaa
     9901 atacacacct gcacaccgcg ccccggcagc cccgccagcg cctcgcgccc cgaaccaaac
     9961 tcggcaacta cctgcaaatc aggttccagc cccagcagct gcgcaaagcc ggagcggacg
    10021 atgaggtgat cgtctataag ggcaacggtg atcatggtct tgtcctggcg ggtaaaaaaa
    10081 aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc cttgagcggt tactgaaaaa
    10141 acaccgcaat cttgttaaac atagtcggat cggactgatt acgctgcact ttcaccacat
    10201 cttccagctt atcgatttgg cttatcatct gctccagacg ctggtcgtca ttgaccagta
    10261 gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca aagaatgcct tcaacgttaa
    10321 aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac gcccggatgg ttgcgaacgg
    10381 tgagctccag aatcacgttg tcatgagttg tgttttgcat ggcttattcc cccaccattt
    10441 cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt ttcttcggca tcaatgcgca
    10501 catggatcag cgccgggcca gggcgattga tgatttcctg caatgcagcc tgcggatcgg
    10561 cttcgttatt caaatcacag gtttcgaggc cgaatccggc ggcaatctgc ataaagttga
    10621 tttttcccgg ataggtggcg gcaaaaacgc cttgctcgta gaacagactc tgttgctgat
    10681 gcaccagccc cagcgcttcg ttgttcatca gaatgatttt gacatccagc tgattttcac
    10741 tggcggtcgc catctcctga atattcatca tcaggctgcc gtcgccggag aaacacaaca
    10801 ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc aggcaggcca aaacccatcg
    10861 tgcccagccc accggaggtc agccactggc gtgggcgatt gagcggataa gcttgcgcgg
    10921 tccacatctg atgctgaccc acatcggtgg tgataatcgc attgtcatcg acacaggcgg
    10981 caacggcgtt gatcaggccg taatggctta atggatcgca cgctttcggg attggacacg
    11041 ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc tgcacgcggt tgcgcttcca
    11101 cctgcgggat caattgcgcc agcacgtcat caacatccgc ctgaatcgcc acatgcggct
    11161 gcttgatttt acccagctct gcgcgatcga tatcgacatg aataattttg gcattcggac
    11221 agaactgctc ggttttgcca atcgcccggt catcaaaacg cgcaccgagc actatcaaca
    11281 aatccgcctc ctgcaaaata tagttggtgc tgcgcacgcc gtgcatcccc agcataccca
    11341 gcgacaacgg atgcgctttt ggcaacatgc ccagcgccat taaagtcatg gtggtaggca
    11401 gttgcgcttt ctccgccagt tcacgcaccc gtgcgggcgc attgatcaca ccgccgccca
    11461 gataaagcac cgggcgtttg gcagcgttaa tcatcgccgc tgcgtcacga atgctttctt
    11521 cgctaaaggc gggggcggcg gctttttctg ccatagcggg ctgtgtttca atctcaaaaa
    11581 ctgccgtttg cacatcctta ggaatgtcta tccacaccgg gcctgggcgg cctgattgcg
    11641 caatgcggaa ggcatcgctc atgacctgcg ggagttcttc gatatgtctg accagatagt
    11701 tgtgtttggt gatggggata gagatgccgt aggtgtccac ttcctggaag gcgtcggtgc
    11761 cgatcatcga ggcgggaacc tgaccggtga tgcaaatcag cgggatggag tccagccgcg
    11821 catcggcaat ggcggtcacc aggttagtcg cacccggtcc gctacaggcc atacagaccg
    11881 ccggtttacc gtcggtgcgc gccatcccct gagcgataaa tcccgcgccc tgttcatgac
    11941 gggccagaat atggcggatt tgcgtgcttt ggcttaaggc atcgtaaaca ggcaggatag
    12001 aaccgcccgg aatgcccgtc acaatcttaa tgccctgctg ttccaggaaa tgaacgataa
    12061 attctgcgcc ggtaaagcgc ttacgcgtcg atgttgtgcc cgaacttgcc atgctccagt
    12121 ccttttcttc tgggccgact ttccgggagg tgcttaaacg aaaaaccccg cccggtttgc
    12181 gccggcgggg ttttggaatc gtgtgttgtt ccagtcccta c
//
LOCUS       DAEVZA010000144        12143 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEVZA010000144 DAEVZA010000000
VERSION     DAEVZA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.144"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..183)
                     /locus_tag="LAF92_004155"
     CDS             complement(<1..183)
                     /locus_tag="LAF92_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HBI9793078.1"
                     /translation="MSELLQKLTRSCFVDRDALDVARTQAALWQTWLLPVTANTPVGE
                     DPGYHDDFLRIRDEMNK"
     gene            complement(176..3502)
                     /locus_tag="LAF92_004156"
     CDS             complement(176..3502)
                     /locus_tag="LAF92_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HBI9793079.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRNVRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLTALKK
                     LRRSRPLDGIIWALTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQA
                     GRPLQSVGCLLPERCTPEQLTAMLEAHTLPLTEQGMSQLLADNRHDFLLRLAHTLAER
                     GIAHWQSVLKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSQVWAGVTGDNAR
                     GRAVGFPWLRAALMSAVCVLAIWGAGMTTSFFANCALVQETSIQTARALDTRLPQAEQ
                     LVALHTLQGELERLQYRIREGTPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLCY
                     ENIPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVANTAFASGDAGIHFELRAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            complement(3559..4773)
                     /locus_tag="LAF92_004157"
     CDS             complement(3559..4773)
                     /locus_tag="LAF92_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793080.1"
                     /translation="MPVRLNTIPDAESYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPHFWGLACGVPAFIWSLVASVRWLFFITQYIRADAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTAAFLARQQVLNTYVDLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVYLEPKTPERTAESSTALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGIWTAELDREQRTALLSL
                     NQPFTQEALMYELDAFLGRSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            complement(4851..5333)
                     /locus_tag="LAF92_004158"
     CDS             complement(4851..5333)
                     /locus_tag="LAF92_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793081.1"
                     /translation="MKTCQLKKNESVQLLSLRTIDGDTLYLKFRNVITGAFLDTPTKS
                     YDFFGDVVLSQCINGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPE
                     AVWFGKNNILIIIRNQMDVGTWKGKYIIYDNAKKSGERAYSSDNLPSKDGYIIFALNK
                     "
     gene            complement(5404..6006)
                     /locus_tag="LAF92_004159"
     CDS             complement(5404..6006)
                     /locus_tag="LAF92_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBI9793082.1"
                     /translation="MADKSEKKKETTNKSAKKTLTKKSEKLTPAKDTAPKQVNVDPAI
                     RLKQIRQLVRNNNKSIMGEDYIICQIYKESRFKQFAGKNKHNAKGLMQMQRNAVRQVF
                     KYRQQKIKGRMTTDKETNEAFANADTFYKSDKIFDEKENIKIGTEYLQYWIDKEATIE
                     EAYRAYRGTDEAYYSVIKPCAEKLAKDPDNIQILMEGIGR"
     gene            complement(6028..8535)
                     /gene="vgrG"
                     /locus_tag="LAF92_004160"
     CDS             complement(6028..8535)
                     /gene="vgrG"
                     /locus_tag="LAF92_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI9793083.1"
                     /translation="MDILVFDHSYHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     STDKAITPELVLMQDGAFSLTAPPVQGMPVQAPLRTLYGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQ
                     YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLHD
                     GATEAVWGLNTAYRVVEKSVSTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADN
                     FLQKGDNEAAESGAFYARIRHERYLNEQAILKGLSTSSLLMPGLEIRVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREMAP
                     AMAILDGAQSQMQSLSTDAQTTNADPADLSSQIALLQQSVKDLTQAVILLSAPKGVAI
                     ASGEHLQLAASKNVIANAGNNADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAVSV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIKSDDFNQSFILLDENTGQPLINWPYELELESGLKM
                     SGITDENGNTELISSDKEEVVNISVFEPDEFLDDDIN"
     gene            complement(8860..9396)
                     /locus_tag="LAF92_004161"
     CDS             complement(8860..9396)
                     /locus_tag="LAF92_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793084.1"
                     /translation="MIVSNAIADDNTKKYFLSERINYVLSLISESKVFNHIENDNLFD
                     SIVIEPVKEGDEIKGALVYKGSKLAILERNSSYSPWSKSSLIVKNYGFHDEDNFFVKN
                     FFDNLNLQFIHRKKNSFKFDVSQYSNNLSKDFCRYYFIWEKDPLVRFSLTSDAVFCEQ
                     QELYPIKIRTITIEKYGS"
     gene            complement(9386..10237)
                     /locus_tag="LAF92_004162"
     CDS             complement(9386..10237)
                     /locus_tag="LAF92_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793085.1"
                     /translation="MGSFNFDDVKTTIGALKKQNDSGEPNEKSVKITKPDRYVLSIGF
                     EVNIERAFQNDTLDLAIDFGHAFLYVTKNNLVTDFFSFGPTKVIAPEGEKSDSIKKRV
                     GMVNFPIKETSKIFRFTITKKQVEKIHSKIKSFSNKVDNKEEYFDVVTNNTCAKVSQL
                     LLEQAGISTPESSSHVKSSKEEDYNWLTETFNFANPYKWYNSFLKQFEHPIGCLGPDG
                     SDALDDTQPAKYYHILRDSWILIPNQSDDPLIANGMVLKDGSGKIHGTAKSGWFSDSL
                     DRGSYDS"
     gene            complement(10239..>12143)
                     /gene="vgrG"
                     /locus_tag="LAF92_004163"
     CDS             complement(10239..>12143)
                     /gene="vgrG"
                     /locus_tag="LAF92_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI9793086.1"
                     /translation="FYDDQSGYERGLTLPLRHPSGLHDGATEAVWGLNTAYSVVEKSV
                     TTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADNFLQKGDKEAAESGAFYARIR
                     HERYLNEQAILQGQSTSSLLMPGLEIRVQGDDAPAVFRKGVLITGVTASAARDRSYEL
                     TFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKNDIYAHIDKDGRYRVNLDFDRD
                     TWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHT
                     DHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKEQRG
                     EGFELRTDLWGAVRAKKGIFISADAQDKAQGQVREMADIISELNGLSDKIQKLSDDAT
                     TANADPADVAAQIALITSRINDLTASVILMHAPKGVAVASGEHLQLAAVKNLQINAGN
                     NADIGVVKNMFIGVGRALSVFVRKAGIKLIANKGAVSVQAQHDLMELLAKKSIEIVST
                     EDEIRISAKKKITINGGGSYIRIEGSGIEPGTPGDYNVKAVHYGRQPGAKLKQEMMAF
                     PMIESGEFNTRFFFTDDNDIPYANTKYIARLPDGTTKDGITSDDGRSEIFFSDKDGEI
                     LIHLFVEQDK"
BASE COUNT         2640 a         3291 c         2970 g         3242 t
ORIGIN      
        1 tttgttcatc tcgtcgcgga tacgcaggaa atcgtcgtgg tagccggggt cttccccgac
       61 cggcgtattt gccgtcaccg gcagcagcca tgtctgccac agcgctgcct gtgttcgcgc
      121 cacatccagt gcatcccggt cgacaaaaca gctacgggtc agcttttgca acagttcact
      181 catcggcgtc ttccccgtat tcttccgcat ctttcatact cctgccggtc agaaatacct
      241 gtcccggcag gcggaagttt ttcagttcca gcagcgccag cggcccttta ccctgttcaa
      301 cccgcagcag gtaattcagc ggcaggccgt cctgcgcttt ccacaccact ttaaaggtgc
      361 tgctgtccac cggtgtcacc tgcgcctgtt ccagcaggcg aataaggctc cagctccccc
      421 ggtaatccgc cagtatgcgt tccatcgcct gcgtactggt ccagctcagc gatgcgccgg
      481 gttgccactg ttcatccggc cagttgaaac gctgccagcg ttctttctgg ttgaaatact
      541 ccagttcctg cccgtcaatc accagatgtg tcttcatcac gtctcgggcc ggttttgccc
      601 gcagttcaaa atgtattccg gcatcgccgc tggcaaaggc ggtgttagcc acgtcgcgca
      661 ggcggttcag ggcgcggata aagtccgggt tgaccgtcag cccctgcgtg ttcatggcgt
      721 ccgccaccca gtagcggcct tcacgtttca gaacgccgga aaggttggcg gcaataaact
      781 ggttgatgcg cccgtcatcg cgcagatact gcgccagcag ggggagggag gcatcgttct
      841 gtgaggcttt gaacgggtag cgaccagcga agtcctgatt ccagtggctg acaatcgccc
      901 gctgccactg gcggttcagg ctgtccgccg caggcgtcag cacctgccgc cacgactgct
      961 ccaccgggcg gacgaacagc gcctgaccga agccactcca ttcctcgccc agactggcgg
     1021 cgattaaccg tccgtagtcg cgggtgtcgg tgaggtcaac ggttttgccc tgcagtaccg
     1081 tctgtgccag ctgttgcgtc atttcctgcg ggtcgggggc gttggtcacc tgttgcagtt
     1141 tgagacgcac acgggtaacg cgggtgaggt aggtctgcag actcagctgg ctgtcgccgc
     1201 ctccggcttt atcgcccagc aaccgcagca gcggagcgaa ggttttatcc agcggcccga
     1261 caggcacgtc gccttcacgg gattgttgcg gcgttttctc cttgccgtta aacagttcct
     1321 gtgccgattt cgccagcgag tctgacaggc cacggttttc cctgcctgcc gccgcctgcc
     1381 acgcgagggt atcggtcagc gcaatcagtg gcgactgacg ggcatcggcc ataagtgtca
     1441 gctggtcgag aatgccggag agcgagtctt cctttttcca gcgaatgctg ttgagaaacg
     1501 ccagccagct tccggcaaag tcggtaaagt agcgtgaggt gagacggtta cgcagcgtat
     1561 ccggcgagat atccgcagag gtatcctgct ggcggtcgct gagtacccag tcgatttcct
     1621 cgcgccgcgc cgtcaccacc tgctcgatgg cttccctgac ctgtccttcc cacgcctgac
     1681 gggtgaacat ccccggcacc gtctgttccg tactgaaaag agattcggtg agggtatccc
     1741 cggtcatgtc cgccagcgtc atatcggcgt agttgcggga cacctgttgc agcacgttct
     1801 ggtagagggt gttttcggca ttacgcacac caatctggcg cagcaggatt ttacgcaccg
     1861 cgccggtcag ttccggtggc ggcgaggttt tccattgcgg gtgttccggc aggttcgcca
     1921 tccagaaggt cagcagcgag ggcaacacgc tctgccgcac accttccggg atattctcgt
     1981 agcacaggcc gtccgccatc agcgtggtac tgaaaaaggc agcgtcggcc ttttccggac
     2041 gggaagtcat cagcaatgcc ttaagctgct tatagcgttg ttcaccggtg gcggtacgct
     2101 gaggactgtt gggcggcagc gcgacaaagg cgttcagttg ctgttgcaga tggtcaacgg
     2161 ccacgtcgcg caccagccgg tttgccgcct gcgcatagcc gggaaaagcg gcggcgagca
     2221 gttgttggtt acgttcaagg ccaaaacgct gataccacgg cgtaccttcg cggatacgat
     2281 attgcaggcg ctccagttcg ccctgcaggg tatgcagcgc caccagttgt tctgcctgcg
     2341 gcaggcgggt atcaagcgca cgtgcagtct ggatgctggt ttcctgaaca agagcgcagt
     2401 tggcgaagaa agaggtcgtc attccggccc cccatattgc cagcacgcag acagcggaca
     2461 tcagcgcggc gcgtagccac ggaaagccca ctgcacgccc ccgtgcgtta tcgccggtca
     2521 cgcccgccca gacctgtgac ggcagccacg catgaggtgc ggcctccggt acggcggtaa
     2581 gcggcgggct gaacatcaga ccacgcaggc gcagggaaga gaatgcgccg cctgccagca
     2641 gcggtttcag gacgctttgc cagtgagcga taccccgctc tgcaagggta tgggccagac
     2701 gcagcagaaa atcatggcgg ttatcagcca gcagttgcga catgccctgt tccgtcagcg
     2761 gcagggtgtg agcttccagc atggcggtca gttgttccgg ggtgcagcgt tccggcagca
     2821 ggcagccgac gctttgcagg gggcgtccgg cctgataatc accgttgtca cagacctgcc
     2881 acaaatagag tggggcctga aaaccgagtc gcttaccgcc gtttatcagt tcgcgccagc
     2941 ctttgtcgag ttgcgctgtc tggcggctct gttcttctgt cagcgcccag atgatgccgt
     3001 ccagcggacg gctgcggcgc agttttttta aggcggtcag cagtgtgaca tcaggctccg
     3061 ctgatggcct gccgccatat atcagcaccg tgtggtcgcc ctccagccag tgttgcccgg
     3121 tcagccccgg cgcgatggct tctgcctgtt caggctcgcc ggtcaccagc agcaggcgga
     3181 cgttacgccg ccagaacgga ccgtagcggt cgcgcaggaa ggcggcgagt tccaccattg
     3241 ccggatgacg gctgtcggct ttctttgtcg tcaccgcacg atagtcatct ccggcggcaa
     3301 gttgtctgcg gagttcaaag ttttgtacgg cagcgtggcg gttcagcaga tgataaaccc
     3361 ccatgataat caaaaagacc acgcctgcgg caatcacgcc agccatccag tacatttgct
     3421 gtgagctacc cggttcaacg cccagcaatg ccggtttacg gtgaataaac gtgcaaagcg
     3481 caaccacaac agccacggtc atgacgatgg caggccctga aacagaacgg ggccggagac
     3541 ggtttaatcc cggaaccact atgatgcctc ctgtggggaa acgaatggcg acaccacggt
     3601 cgcccatgaa taatgcccgc cctgaacgcc gctgagcgtt agttgggggt gttgtgattg
     3661 tattgccgcc agcgttgccg cagcgacgga cagccacggc gctgccggtc cggagcgccc
     3721 cagaaaggca tccagctcgt acatcagggc ttcctgagta aacggctggt taagggataa
     3781 caacgcggta cgctgttccc tgtccagttc ggcagtccat atgccggata tggcatctgg
     3841 ctgcaccggc atccagtcca gcgcctgtgc aatgcctgac gccatcatct ctgtatctgt
     3901 gatacgttca ggacgatgca gcagggccag cggcgtcagc gcggtttgcg tcaaccggtt
     3961 tgccagcaac agcgcggtgg aggattctgc cgtccgttcc ggagtcttcg gttccagata
     4021 cacggaaatc accagcagta cggccttttc ctgaataaag ttgtccagcc agtcgtccac
     4081 ggcggccagc cccggctccg gggcagaaaa cagacgggct tccgggagtc cggctttttg
     4141 ccattcgtcc tgccagatag ttctcacgga ggcccctgaa agcggcgcat caatttccag
     4201 tagtacgttg atatgaaaat cagggggcag cagaaacaat tgcggtctga tatcaataaa
     4261 aaggcgggaa ataatttcgg ataaacgccc ggtaaccggt ttattggtta atacgggaat
     4321 gacactgcga cgcacggtat cttctccgcg caaatcaacg taagtattca acacctgctg
     4381 acgcgccaga aacgccgctg tggtttctgc gacggaatca ccattcagcg cggtttgtac
     4441 ggaaaaacta agtatctgca acgcccgtcg tccccgtcgc gtttcctgca aaatcacctc
     4501 ttcccggcgc ttattccagg catcagcccg aatgtactgc gtgataaaaa acaaccagcg
     4561 aactgaagca accagcgacc agataaaggc aggcacaccg caggccagcc cccagaaatg
     4621 cgggccgttt tttgccagtt catcgctggc gaataatgag gttattgcca caccaacaga
     4681 cagcatcagt aataaggctg acagccattt aatgcgggct ggcggtgcgg gataggattc
     4741 tgcgtcggga atggtattaa gtcggacagg catcagtaca tccctttatt ttgcattatc
     4801 ggccagaagt taataaccaa gtgaaattta aagttatcta tagagtcatt ttatttattt
     4861 aatgcaaaaa ttatgtaacc atcttttgat ggtagattat ctgatgagta tgctctttct
     4921 cctgactttt tcgcattatc atagataata tatttccctt tccatgtccc aacatccatt
     4981 tggtttcgga tgataattaa aatattgttt tttccaaacc ataccgcttc gggaatattt
     5041 ctctctgcga aacaaaagcc ttcaggagag ttcttacctt taaacccatt ttcccaacca
     5101 gttaccagac aaccttttat atatggagaa ccatattcca gcgcaaagat aagagaacca
     5161 tttatgcact gagaaagaac cacatctcca aaaaaatcat aacttttagt tggagtatct
     5221 aaaaaggccc cggtgataac atttctgaat tttagatata atgtatcacc gtcaatggtt
     5281 ctaagggaaa gcaactgcac actttcattt tttttcagtt gacaggtttt cacgatttta
     5341 atcttatcgc ctgaaaaaca ggggaaagaa aaaaaacaaa gtaatattaa cataatgcgc
     5401 atactatctt cctattccct ccattagtat ttgtatgtta tcaggatctt tagccagttt
     5461 ttcagcgcaa ggttttataa cagaatagta tgcttcatct gttccacgat atgcccgata
     5521 tgcctcctca attgttgctt ctttatctat ccagtattgc aaatactctg tgccaatctt
     5581 aatattttct ttttcatcaa atattttatc agatttataa aatgtatcag cattagcgaa
     5641 agcttcattt gtctctttat cagtcgtcat tcttcctttt attttttgtt gtcgatattt
     5701 aaaaacctgc ctcactgcat ttcgttgcat ttgcattaag cctttcgcat tatgtttatt
     5761 cttacctgca aattgcttaa atctagattc tttatagatt tgacaaataa tataatcctc
     5821 tcccatgatg gatttattat tattacggac aagttgacga atttgtttca atcttatagc
     5881 tggatctaca ttcacttgtt ttggtgcggt gtcttttgct ggagttaatt tttcgctctt
     5941 cttcgttagt gttttttttg ctgatttatt tgtggtttct tttttctttt cggatttatc
     6001 tgccatgtta tattctcgct atatgtctta attaatgtca tcgtctaaaa actcatcggg
     6061 ttcaaacaca ctaatattaa cgacctcctc cttatcagag gatattagtt ctgtatttcc
     6121 attttcatca gtaatcccac tcatttttag cccactttcc agttctagtt catagggcca
     6181 attaataagt ggttgcccag tattttcatc cagcaaaata aagctctgat taaaatcgtc
     6241 agacttaatc accggaaatg ccatcagctc cggcgtcagc ttcgcctttg gtttacgccc
     6301 gtaatatccg gccttaacgt tatattctcc cggcgttccc gcttctatcc cgcaggcatc
     6361 cagccggatg tagctgccac cgccgttaag ggtgattttc ttcttcgcgg tgatcttgat
     6421 ctcatcttcc gtactggtga tctctatcga tttctgtgcc aacagttcca tcagatcgtt
     6481 ctgtgcctgt accgagaccg cccctttatt ggcaaacagc ttgatgcccg ctttacgcac
     6541 aaacacgctc agcgcctgac ccacaccgat aaacatattt tttactacgc cgatatctgc
     6601 gttgttgcct gcgttcgcta tcacattctt cgacgccgcc agttgcaggt gctcgccgct
     6661 ggcgatcgcc acgcccttgg gtgcacttag caggatcacg gcctgcgtca gatcttttac
     6721 actttgctgc aacagcgcta tctgcgatga cagatctgcc ggatcggcat tcgtcgtctg
     6781 cgcatcagtg gacagcgact gcatctgtga ctgtgcgcca tccagtattg ccatcgccgg
     6841 agccatttcg cgcacctgcc cctgcgcctt atcctgcgca tcggcgctga taaatatccc
     6901 tttcttcgcc cgcaccgcgc cccacatatc ggtacgcagt tcaaagcctt ccccgcgctg
     6961 ttgttttccg gcatccacca gatgacccag attcaactgg ctcttgccgc cgtactcggt
     7021 actgaccttg atatgctctt tgccgcgctc atcgtcgagg cggattttgt tgttcgccgg
     7081 ggtgcgcaga acgttgcgct tgtagttctg gatggtcaca tggtcagtgt gtgccgagtc
     7141 gtgttttacg cctgcaatat aaggtcggtc cgggttgcct tcctcaaagg cgatgctgac
     7201 ttccgttccc gccagcagcg gcagatgcag gccgtaggtg tcaccggcat acgggcgcga
     7261 ctggcgtacc cacaggcttt cataacccgg tttccacgta tcgcggtcaa agtcgaggtt
     7321 gacccggtag cgcccgtcct tgtcgatatg agcataaata tcgttcttca ctgtgctggt
     7381 gacccgcgcc gggagcgtgc cggccattac cgggcggggg ataagcgccg ggcggtagcc
     7441 gtagcgttct gagtagggta tggccgtgaa cgtcagttca tagctgcggt cacgcgccgc
     7501 gctggccgtc acgccggtaa tcagcacgcc cttgcggaat accgcaggcg cgtcgtcgcc
     7561 ctgaaccctg atttccagcc ccggcatcag cagtgaactg gtgctcaggc ctttgagaat
     7621 ggcctgttca ttcaggtagc gttcgtggcg gatacgggcg taaaacgcgc cgctttccgc
     7681 tgcctcgtta tcgccttttt gcaggaagtt gtcagcatag tggtaggctt cgccgtaggt
     7741 ggtgttatcg cccccggtgg cgtcatgctg ctctgtcatc atctccgctg tggcggtgcg
     7801 gtagttgtag tcccgcgtgg agacgctttt ttccaccacc ctgtacgcgg tgttcagtcc
     7861 ccataccgct tccgtggcgc cgtcgtgtaa ccctgaagga tggcgaagcg gcagcgtcag
     7921 tccgcgctca tagccgctct ggtcgtcgta aaactcgata acctcaattt tcagccgggc
     7981 atcggtggcg aagcggaacc agatacccac ttctgacagc aggcgcgaga caaacgtcag
     8041 gtcgtcctcg ccatactgca tcacctgttc gcgggccggg tattcgcttt tgaggttaaa
     8101 cacgaagtcc tgaccgcgca tctggtgccg ctcgcgcagg attttttcga cgatttgcgg
     8161 cacagtctgg ttctggtaaa tggcattctg gcggctgcgg gtcagcagcg ccatacgggg
     8221 ttcaagccgt acttcgtaac gggcttcgtc ctgtgacgat gagagatgtt taaagccggt
     8281 gatgacgccg tacagggtgc gcaggggtgc ctgcaccggc attccctgta ccggcggtgc
     8341 cgtcaggctg aatgcgccgt cctgcatcag caccaactcc ggcgtgatgg ctttatcggt
     8401 actggtaaac tgaatgtcgt agcggaacgg ggtactcagt tgttcgcggc cttcgaaggt
     8461 cagcacatcc accggacttt gcaggccgcg gattttgagt ttgtggtacg agtggtcaaa
     8521 aacaagtatg tccatgatgg cctctattat tttattttgt atacatagat agttattatt
     8581 ttcttatttt tgtataattg atacatgata aatatatatt ttgaaacagg gcttgttatt
     8641 caccattgga ttatccaaac ggttttatag agtgtactca aatatacatt tgtaactcat
     8701 attaacttga gcggttaatt ttacaataaa ataaaagaag ctattttaag ggggtattac
     8761 tgttctaaag caaccagtca tactattact acttaccacc tatcatttca tgataggctc
     8821 caatgcttcc caatatatgc ttttatagtt gtaaaaatat tatgatccat atttttcaat
     8881 ggttatagtt ctaatcttaa taggatataa ttcctgctgt tcacaaaaaa cggcgtcgct
     8941 tgttaatgaa aaacgtacca gagggtcttt ctcccaaatg aaatagtatc tacaaaaatc
     9001 tttactaaga ttattagaat attgtgaaac atcaaattta aaactatttt ttttacgatg
     9061 aatgaattgt agatttaaat tatcaaagaa gttctttaca aaaaaattat cttcatcatg
     9121 aaatccataa tttttaacta ttaaagatga cttactccag ggagagtaac tactattcct
     9181 ctccagtatt gctaatttac tacccttata tacaagtgcc cccttaatct cgtcaccttc
     9241 ttttaccggt tcgataacta tagaatcaaa aagattatca ttttctatat gattaaaaac
     9301 tttactttca gaaatcaaag acaatacata attaattcgt tcagaaagaa aatatttctt
     9361 agtgttatca tctgctatag cattgctaac tatcatagct ccccctatca agagagtccg
     9421 aaaaccaacc actttttgca gtcccatgta ttttcccaga cccatccttc aaaaccatac
     9481 catttgctat tagcggatcg tcactttgat taggtatcaa aatccaacta tccctcaaaa
     9541 tatgatagta cttcgcaggt tgtgtgtcat caagcgcatc gctaccatca ggccctaaac
     9601 aaccaatcgg atgttcgaat tgttttagaa aactattata ccatttataa ggatttgcaa
     9661 aattaaatgt ctctgttagc cagttataat cttcttcttt actgctcttc acatggctgg
     9721 aactctcagg ggttgatata ccagcctgct ccagaagcaa ctgtgatacc ttagcgcaag
     9781 tgttattggt gaccacgtca aaatattctt ctttattatc aactttgtta ctgaaagact
     9841 ttattttgga atgaatcttt tcaacctgtt tctttgttat ggtaaaacgg aaaattttgg
     9901 atgtttcttt aatagggaaa tttaccatac cgactctttt ttttatgcta tcgctttttt
     9961 ccccttctgg tgcaataact ttggttggcc caaaactaaa aaaatcagtt accaaattat
    10021 tttttgttac atacaaaaac gcatgaccaa aatcaatcgc cagatcaagt gtatcattct
    10081 gaaatgccct ttctatattg acttcaaaac caatagacag tacgtagcga tctggttttg
    10141 taatctttac actcttctcg tttggctcac ccgaatcatt ttgttttttt aatgctccaa
    10201 tagttgtttt aacatcatca aaattaaagc tacccatgtt atttatcctg ctcgacaaat
    10261 agatgtatta atatttcccc atccttatca gaaaaaaata tttctgatcg accatcatcg
    10321 cttgtaatgc cgtcttttgt tgtaccatct ggtaatcttg ctatatattt ggtgtttgca
    10381 taaggaatat cattatcatc tgtaaaaaag aaacgggtat taaattctcc agattcaatc
    10441 atcggaaacg ccatcatttc ctgtttcagc ttcgctcccg gctgacgccc ataatgcacc
    10501 gctttgacgt tataatcccc cggcgttccc ggctcaatgc cgctaccctc tatccggata
    10561 taacttccac cgccattaat cgttattttt ttcttcgctg aaatcctgat ctcgtcctca
    10621 gtactgacga tctctatcga ttttttcgcc agtaactcca tcagatcgtg ctgcgcctgt
    10681 accgatactg cgcctttatt ggcaatcagt ttgatacccg ccttgcgcac aaacacgctc
    10741 agcgcccgcc ccacgccgat aaacatattc ttcaccacgc cgatatcggc gttgtttccc
    10801 gcatttatct ggaggttttt caccgctgcc agttgcaggt gctcgccgct ggcgaccgcc
    10861 acgcctttcg gcgcgtgcat caaaatcacc gaggcggtca ggtcatttat ccggctggtt
    10921 atcaatgcta tctgcgcggc cacatccgcc gggtcggcgt tcgccgtcgt cgcgtcatcg
    10981 gacagtttct ggattttatc gctcagaccg ttcagttcgc tgataatgtc ggccatctct
    11041 ctgacctgac cctgcgcctt gtcctgcgca tcggcgctga taaatatccc tttctttgca
    11101 cgcaccgcgc cccacagatc ggtacgcagt tcaaagcctt cgccgcgctg ttctttcccg
    11161 gcatcaacaa gatggccgag gtttagctgg ctcttgccgc catattcggt actgaccttg
    11221 atatgctctt tgccgcgctc gtcatccagc cggattttgt tgttcgccgg ggtgcgcagc
    11281 acgttgcgct tgtagttctg aatggtcaca tggtcggtgt gtgccgagtc gtgttttacc
    11341 ccggcgatat acggtcggtc cgggttgcct tcctcaaagg cgatgctgac ttccgttccc
    11401 gccagcagcg gcagatgcag gccgtaggtg tctcccgcat acgggcgcga ctggcgtacc
    11461 cacaggctct cataacccgg tttccacgta tcgcggtcaa aatcgaggtt gacccggtag
    11521 cgcccgtctt tgtctatatg ggcataaata tcgtttttca ccgtgctggt gacccgcgcc
    11581 gggagtgttc cggccattac ggggcggggg ataagcgccg ggcgatagcc gtagcgttct
    11641 gagtagggta tcgccgtgaa cgtcagttca tagctgcggt cacgggccgc gctggccgtc
    11701 acgccggtaa tcagcacgcc cttgcggaat accgcaggcg cgtcgtcgcc ctgaaccctg
    11761 atttccagcc ccggcatcag cagtgaactg gtgctctggc cctgcagaat ggcctgttca
    11821 ttcaggtagc gttcgtgacg gatacgggca taaaacgcgc cgctttccgc tgcctccttg
    11881 tcgccttttt gcaggaagtt gtcagcgtag tggtaggctt cgccataggt ggtgttatcg
    11941 cccccggtgg cgtcatgctg ctctgtcatc atctctgctg tggcggtgcg gtagttgtag
    12001 tcccgcgtgg tgacgctttt ctccaccacg ctgtacgcag tgttcagccc ccatacggct
    12061 tccgtggcgc cgtcgtgtag ccctgaagga tggcgaagtg gcagcgtcag tccgcgctca
    12121 tagccgctct ggtcgtcgta aaa
//
LOCUS       DAEVZA010000145        12014 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEVZA010000145 DAEVZA010000000
VERSION     DAEVZA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.145"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..500
                     /locus_tag="LAF92_004164"
     CDS             <1..500
                     /locus_tag="LAF92_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBI9793087.1"
                     /translation="QGAHAAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSN
                     KLFRRCSARDMLLISAICGVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIA
                     ARQGSEVIRLQAVYSAVAMGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPK
                     VVPSC"
     gene            complement(492..1772)
                     /gene="csiE"
                     /locus_tag="LAF92_004165"
     CDS             complement(492..1772)
                     /gene="csiE"
                     /locus_tag="LAF92_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBI9793088.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSFF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(1963..2817)
                     /locus_tag="LAF92_004166"
     CDS             complement(1963..2817)
                     /locus_tag="LAF92_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI9793089.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(2962..3765)
                     /gene="suhB"
                     /locus_tag="LAF92_004167"
     CDS             complement(2962..3765)
                     /gene="suhB"
                     /locus_tag="LAF92_004167"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBI9793090.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            3884..4624
                     /gene="trmJ"
                     /locus_tag="LAF92_004168"
     CDS             3884..4624
                     /gene="trmJ"
                     /locus_tag="LAF92_004168"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBI9793091.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            4894..5382
                     /gene="iscR"
                     /locus_tag="LAF92_004169"
     CDS             4894..5382
                     /gene="iscR"
                     /locus_tag="LAF92_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBI9793092.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            5494..6708
                     /gene="iscS"
                     /locus_tag="LAF92_004170"
     CDS             5494..6708
                     /gene="iscS"
                     /locus_tag="LAF92_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBI9793093.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            6736..7122
                     /gene="iscU"
                     /locus_tag="LAF92_004171"
     CDS             6736..7122
                     /gene="iscU"
                     /locus_tag="LAF92_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBI9793094.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            7139..7462
                     /gene="iscA"
                     /locus_tag="LAF92_004172"
     CDS             7139..7462
                     /gene="iscA"
                     /locus_tag="LAF92_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBI9793095.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            7558..8073
                     /gene="hscB"
                     /locus_tag="LAF92_004173"
     CDS             7558..8073
                     /gene="hscB"
                     /locus_tag="LAF92_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBI9793096.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            8090..9940
                     /gene="hscA"
                     /locus_tag="LAF92_004174"
     CDS             8090..9940
                     /gene="hscA"
                     /locus_tag="LAF92_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBI9793097.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            9942..10277
                     /gene="fdx"
                     /locus_tag="LAF92_004175"
     CDS             9942..10277
                     /gene="fdx"
                     /locus_tag="LAF92_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBI9793098.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            10289..10489
                     /gene="iscX"
                     /locus_tag="LAF92_004176"
     CDS             10289..10489
                     /gene="iscX"
                     /locus_tag="LAF92_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBI9793099.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            10667..11950
                     /gene="pepB"
                     /locus_tag="LAF92_004177"
     CDS             10667..11950
                     /gene="pepB"
                     /locus_tag="LAF92_004177"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBI9793100.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVVWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
BASE COUNT         2886 a         3006 c         3385 g         2737 t
ORIGIN      
        1 tgcagggggc acatgcggcc tattacggtt ttagcgccat ttactggcag gcagctggct
       61 actcggcctc ggcggtgggg tatttgtggt cgctgggcgt ggtggcggaa gtcattatct
      121 ttgcgctgag taataaactt ttccgccgtt gtagtgcacg cgatatgctg ttgatctcgg
      181 cgatttgcgg cgtagtgcgc tggggcatta tgggagcaac tacggcgttg ccgtggttga
      241 tagtggtgca aattctgcat tgcggcacct tcacggtctg ccacctggcc gccatgcgtt
      301 atattgctgc tcgccagggt agcgaagtca tccgtttaca ggcggtttac tctgccgtcg
      361 cgatgggcgg cagtatcgct atcatgaccg ttttcgccgg tttcctgtat caatatctgg
      421 gccacggcgt gttctgggta atggcgctgg tggcgctgcc ggcaatgttt ttgcgcccga
      481 aagttgttcc ctcatgctga ttcgagcatt ttgcggatct gctcctgttg atgtgttgtc
      541 agcgtcaggt ccgtatagat cagcggtggt gaaaagagcg gtaatggcat ggtataaggc
      601 gcaataatca gtgccgcgcc acgcggagcg cctgtctgca aaaatgcctt taccgacata
      661 tgcttaatgt tgagcggcag taacgttagt tcgcgtaact gctgctcaat ctgcgcttct
      721 cgctcgctat cattcccggt cagtagaata atctgttttt catgcagatc gttttcctgc
      781 attagccagg caccgaaaat caccgcgacc agaccacttt cctcatcaga aagatggacg
      841 ccgtattcac tttcaaatcc ggccagcgcc gcgcgggtgg tgcgcaccag gcgtgggtac
      901 agtctggcga actcttccgg cagggtatta tcaataccga tggcaaaaaa actgcgattt
      961 aacgcctggg cgaggtgggt ataaagctga tcgcataacc cctgttcatc ataaaaacga
     1021 acatttccca gctcacgaaa atggtttacc agacgtttga tagactgacg caattgtctg
     1081 tctcgctgat gcgcatcacg caatggatcg ggaacccgca acatggaaaa aagtaatgcc
     1141 ataaacagtg gctcatcagg cggtacaggc tggagcgccc gacgctgcca gtggcgtcca
     1201 atttcctgcg ctacctgaaa ttcaaggcag gattccgccc agcgacgttg gagcggatta
     1261 aactgaggcg taattccagc gtggtgttgc agcaaacaat attgcagata cagacacagg
     1321 aaatgaatat cgcgcgtttc aaagcgtttt tgcagccgtc gggagcagag attcactaac
     1381 gcttgtagat tggtatcgtc gtaaaagtta cgcgcaattc cgcgccgctt cagttcactc
     1441 ttcagggcgg gggtgaaatg gctggtaata aatgatggac acagacggaa accacgtcgt
     1501 agccagtgaa ataaacacaa acgttggtta agcactgtac cttcaacccg gtagctaccg
     1561 tcataaccag ccgtcagtgt gagttgatga tagcgcagga tctcctgtcc tgtttcgctg
     1621 atatcaagac tggcaatatc atcatccaca ccattaagct cgctgaaagt tgccgtggtg
     1681 gcggtcaacc ccggctgaaa gagcgtcagc aagatctggc agcggcgctg gggagccgaa
     1741 aggacagatg gtggagcaag cgtaggcatc atctgtagat ctcccataag cataattgat
     1801 aagaatagct aaagtttgct cagggaaatg gcgaagggaa tgctaatcat cagaaatgtt
     1861 ggcaagcgtc acagaatttt tctcatctta aactgttctg accagtggat tagcatgtct
     1921 taaggtggtt ttgtgaatga ttatcgtgct agcgatcatg ggttagttct gtggattcaa
     1981 cgcgctaagg ataaagtcca ccatctgttc gcgataaaca tcgccgtgac gatcggaaaa
     2041 agcatcaatg tgttcgccat ccgggattag gatcaaccgt tttggctctt ttgccaggct
     2101 atacaacttt tcgctgtgct gccatgggat aacgtgatca gctttaccgt gaatgagtaa
     2161 aagcgggatc gggctgacgc tggcgatata attttcgccg ctgtaactct catcaagtaa
     2221 gtagccactg ccggggatca tctggttggc gatggttgca taagaggcaa atgtggagtc
     2281 gaggatcacc gcacgtatgc cttcacgatc accccgacca ataacatcca gaatattcgc
     2341 cccgccaata ctctgcccga acagcaccag acgttgtggg tttacatcac tgcgatggcg
     2401 caccacattg atggcacttt gcgtatcgtc cagcaatccg gcttgggacg gcgtgccttt
     2461 tgatttacca aacccgcgat aatcaaacat aaaaacgttg aaattacgct caggtaacca
     2521 actgaccagc ggccagtggg cggacatatt tccggcattg ccgtgagcat gaatgatggt
     2581 tgcgatggcg ttgtcagcag ggcccgtcga agaaggaata aaccagcctt gcagacgagt
     2641 accgtcctta gccgtaaatt cgacggattc cgccgaccag gggtcgggac cgtaaatttt
     2701 atcgtcagga taatagaaga agttgatggc aacgcgcggc actaaataaa cgcagaaggc
     2761 aacaacaaag agaataaaca gaattttggg aacgcgtttg ttcactggca gtgccataac
     2821 ctttccttgc gacttcttta tcatccatta atcggggaaa gatggtggga gagtgacgaa
     2881 agaaagtaaa gcggtgagga agtatttcag aatagcgcct gaaaggcgag ggcgggtgag
     2941 tgacatcacc cgcctgagtc attaacgctt cagagcgtcg cttaactcgt cacgcatgtt
     3001 cgccagcatg gctttcacaa cacgtgggtt accagcaacg atgttaccgg tcagcatata
     3061 gttatgacca ccggtgaagt cgctgacgat gccgcccgct tcacgaacca gcagctcgcc
     3121 tgccgcgaaa tcccacggac gcagaccgat ttcaaagaaa ccgtcaacac gacccgcagc
     3181 gacgtaagcc agatccagcg ccgcagaacc ggtacgacgg aagtctgcac attcgttgaa
     3241 cagtttgccg acgatgttga tgtaggtagt ggcgtactgt tttgctttga acgggaagcc
     3301 ggtcgccaga atggtaccgt cgagatcgcg agcggtgcta ccgcgcagac ggtagccgtt
     3361 cagctgtgcg ccctgaccgc gagtggcggt gaacagttcg ttacgcatag gatcgtatac
     3421 cacagcaact tcggtgcggc ctttgatacg aacggcgata gataccgcga agtgcggcag
     3481 acgtttgata aagttggtag taccatccag tggatcgata acccattgaa catcctgatc
     3541 agtaccttca agttcaccgc tttcttcggt gatgatggtg tgctgtgggt aagatttacg
     3601 aatcgtgtcg ataatcaccg cttcggcagc tttatctacg ttggtcacga aatcgttact
     3661 gcctttctgg ctcgcttcta cagcgtccgg ggtttcatag tttttggcaa ttaaattacc
     3721 cgccttgcgc gctgcgcgca cggcgatgtt cagcatcgga tgcatcggtc tctctcactg
     3781 gatgttaaag aacgggaaaa cggcgcagag tatagcagcg agatcggcat ctgtcttcgt
     3841 tttatgataa gatgcgtgaa taatctttag atgaagaaag acaatgctgc aaaatattcg
     3901 aattgtgctg gtggagacgt cacacaccgg caatatgggt tctgttgccc gtgccatgaa
     3961 aacaatggga ttaaccaatc tgtggctggt taatccactg gtgaaacccg actcccaggc
     4021 gattgccctg gcagcagggg ccagcgatgt gattggtaat gctcacatcg tcgatacgct
     4081 cgacgaagcg ttagccggtt gtagcctggt ggtgggcacc agcgcacgct cccgcacgct
     4141 gccatggccg atgctcgacc cgcgcgaatg cggcctgaaa agcgtcgctg aagcggcaaa
     4201 taccccggtg gcgctggtgt ttggtcgcga gcgcgtcggc ttgaccaatg aagagttgca
     4261 gaaatgccat tatcatgtcg cgattgcggc taacccggaa tacagctcgc tgaacctggc
     4321 gatggcggtt caggttattg cctatgaagt gcgcatggcc tggctggcaa ctcaggaaaa
     4381 cggcgagcag gtcgagcatg aagagacgcc gtatccgctg gtcgatgatc tggagcgttt
     4441 ttatggtcat ctggaacaaa cgctgctggc aaccggtttt atccgtgaaa accatccggg
     4501 gcaggtgatg aataaattgc gccgtctgtt tacccgtgcg cgcccggaaa gccaggaatt
     4561 gaatatcctg cgcgggattc tggcttctat tgagcagcag aataaaggta acaaggccga
     4621 ataacagtag ttgcctgatg cgacgcttaa cgcgtcttat caggcctaca atgtacagaa
     4681 ccgtaggtcg gataaggtgt tcacaccgca tccgacaaca ggtacaaacg ccacgataaa
     4741 aaaatggcac tgaaggttaa atacccgact aaatcagtca agtaaatagt tgaccaattt
     4801 actcgggaat gtcagacttg accctgctat gcaatacccc cacttttaca ataaaaaacc
     4861 ccgggcaggg gcgagtttga ggtgaagtaa gacatgagac tgacatctaa agggcgctat
     4921 gccgtgaccg caatgcttga cgttgcgctc aactctgaag cgggcccggt accgttggct
     4981 gatatttccg aacgtcaggg aatttccctt tcttatctgg aacaactgtt ttcccgtctg
     5041 cgtaaaaatg gtctggtttc cagcgtacgt ggaccaggcg gtggttatct gttaggcaaa
     5101 gatgccagca gcatcgccgt tggcgaagtg attagcgccg ttgacgaatc tgtagatgcc
     5161 acccgttgtc agggtaaagg cggctgccag ggcggcgata aatgcctgac ccacgcgctg
     5221 tggcgtgatt tgagcgaccg tctcaccggt tttctcaaca acattacttt aggcgaactg
     5281 gttaataacc aggaagtgct ggatgtgtct ggtcgtcagc atactcacga cgcgccacgc
     5341 acccgcacac aagacgcgat cgacgttaag ttacgcgctt aataaaaaga attcagaatc
     5401 aggccggagt gctaaatact ccgtaaacac ggtcgtacat ccagccggta gcctgattcc
     5461 ttgcattgag tgatgtacgg agtttataga gcaatgaaat taccgattta tctcgactac
     5521 tccgcaacca cgccggtgga cccgcgtgtt gccgagaaaa tgatgcagtt catgacgatg
     5581 gacggaacct ttggtaaccc ggcctctcgt tctcaccgtt tcggctggca ggctgaagaa
     5641 gcggtagata tcgcccgtaa tcagattgcc gatctggtcg gcgctgatcc gcgtgaaatc
     5701 gtctttacct ctggtgcaac cgaatctgac aacctggcga tcaaaggtgc agccaacttt
     5761 tatcagaaaa aaggcaagca catcatcacc agcaaaaccg aacacaaagc ggtactggat
     5821 acctgccgtc agctggagcg cgaaggtttt gaagtcacct acctggcacc gcagcgtaac
     5881 ggcattatcg acctgaaaga acttgaagca gcgatgcgtg acgacaccat ccttgtttcc
     5941 atcatgcacg taaataacga aatcggcgtg gtgcaggata tcgcggctat cggcgaaatg
     6001 tgccgtgctc gtggcattat ctatcacgtt gatgcaaccc agagcgtggg taaactgcct
     6061 atcgacctga gccagttgaa agttgacctg atgtctttct ccggtcacaa aatctatggc
     6121 ccgaaaggta tcggtgcgct gtatgtacgt cgtaaaccgc gcgtacgcat cgaagcgcaa
     6181 atgcacggcg gcggtcacga gcgcggtatg cgttccggca ctctgcctgt tcaccagatc
     6241 gtcggaatgg gcgaggccta tcgcatcgca aaagaagaga tggcgaccga gatggaacgt
     6301 ctgcgcggcc tgcgtaaccg tctgtggaac ggcatcaaag atatcgaaga agtttacctg
     6361 aacggtgacc tggaacacgg tgcgccgaac attctcaacg tcagcttcaa ctacgttgaa
     6421 ggtgagtcgc tgattatggc gctgaaagac ctcgcagttt cttcaggttc cgcctgtacg
     6481 tcagcaagcc tcgaaccgtc ctacgtgctg cgcgcgctgg ggctgaacga cgagctggca
     6541 catagctcta tccgtttctc tttaggtcgt tttactactg aagaagagat cgactacacc
     6601 atcgagttag ttcgtaaatc catcggtcgt ctgcgtgacc tttctccgct gtgggaaatg
     6661 tacaagcagg gcgtggatct gaacagcatc gaatgggctc atcattaatc ggtatcggaa
     6721 tcaggagaat ttataatggc ttacagcgaa aaagttatcg accattacga gaatccgcgt
     6781 aacgtgggtt cctttgacaa caacgacgag aacgtcggca gcggcatggt gggtgcaccg
     6841 gcctgtggcg acgtgatgaa gttgcagatt aaagtcaacg atgaaggtat cattgaagac
     6901 gcgcgtttta aaacttacgg ctgcggttct gctatcgctt ccagctccct ggtcaccgaa
     6961 tgggtgaaag ggaagtctct cgacgaagcg caggcgatca aaaacaccga tattgctgaa
     7021 gaacttgaac tgccgccggt gaaaattcac tgttctattc tggcagaaga cgcgatcaaa
     7081 gccgccattg cggactataa aagcaaacgt gaagcaaaat aagagttgag gtttggttat
     7141 gtcgattaca ctgagcgaca gtgcagcagc gcgagtaaat acctttctgg ctaaccgcgg
     7201 caaagggttt ggcctgcgtc tgggcgtgag aacctccgga tgttcaggta tggcttatgt
     7261 actggaattt gttgacgaac cgacgccgga agacatcgtg tttgaagaca aaggcgtgaa
     7321 agtcgtggtc gatggcaaaa gcctgcaatt tctggacggt acgcagctgg acttcgtaaa
     7381 agaaggcctg aacgaagggt ttaaattcac caacccgaac gtcaaagatg agtgtggttg
     7441 cggcgaaagc ttccacgttt gatgcgcata ccgataaccc caccgtggtc gcctgcgcgt
     7501 ggggtttgtt ttacctgatt cgccgttatc gcggcggatc gcagccctga gaatgttatg
     7561 gattacttca ccctctttgg cttgcctgcc cgttatcaac tcgataccca ggcgctgagc
     7621 ctgcgttttc aggatctaca acgtcagtat catcctgata aattcgccag cggaagccag
     7681 gcggaacaac tcgccgccgt acagcaatct gcaaccatta accaggcctg gcaaacgctg
     7741 cgtcatccgt taatgcgcgc ggaatatttg ctttctttac acggttttga tctcgccagc
     7801 gagcagcata ctgtgcgcga caccgcgttc ctgatggaac agttggagct gcgcgaagag
     7861 ctggacgaga tcgaacaggc gaaagatgaa gcgcggctgg aaagctttat caaacgtgtg
     7921 aaaaagatgt ttgatacccg ccatcagttg atggttgaac agttagacaa cgaggcgtgg
     7981 gacgcggcgg cggataccgt gcgtaagctg cgttttctcg ataaactgcg gagcagtgcc
     8041 gaacaactcg aagaaaaact gctcgatttt taatttctgg aagctaaaca tggccttatt
     8101 acaaattagt gaacctggtt tgagtgctgc gccgcatcag cgtcgtctgg cggctggtat
     8161 tgacctgggc acaaccaact cgctggtggc gacagtgcgc agcggtcagg ccgaaacgtt
     8221 agccgaccat gaaggccgtc acctgctgcc atctgttgtt cactatcaac agcaagggca
     8281 ttcggtgggt tatgacgcgc gtactaacgc agcgcttgat accgccaaca ccattagttc
     8341 tgttaaacgc ctgatgggac gctcgctggc tgatatccag caacgctatc cgcatctgcc
     8401 ttatcaattc caggccagcg aaaacggcct gccgatgatt gaaacggcgg cggggctgct
     8461 gaacccggtg cgcgtttctg cggacatcct caaagcactg gcggcgcggg caactgaagc
     8521 cctggcaggc gagctggatg gtgtagttat caccgttccg gcgtactttg acgatgccca
     8581 gcgtcagggc accaaagacg cggcgcgtct ggcggggttg catgttctgc gcttacttaa
     8641 cgaaccgacc gctgcggcta tcgcctacgg gctggattcc ggtcaggaag gcgtgatcgc
     8701 tgtttatgac ctcggtggcg ggacgtttga tatttccatt ctgcgcttaa gtcgcggcgt
     8761 gtttgaagtg ctggcaaccg gcggtgattc cgcgctcggc ggcgatgatt tcgaccatct
     8821 gctggcggat tacattcgcg agcaggcggg cattcctgat cgtagcgata accgcgttca
     8881 gcgtgaactg ctggatgccg ccattgcagc caaaatcgcg ctgagcgatg cggactccgt
     8941 gaccgttaac gttgcgggct ggcagggcga aatcagccgt gaacaattca atgaactgat
     9001 cgcgccactg gtaaaacgaa ccttactggc ttgtcgtcgc gcgctgaaag acgcgggtgt
     9061 agaagctgat gaagtgctgg aagtggtgat ggtgggcggt tctactcgcg tgccgctggt
     9121 gcgtgaacgg gtaggcgaat ttttcggtcg tccaccgctg acttccatcg acccggataa
     9181 agtcgtcgct attggcgcgg cgattcaggc ggatattctg gtgggtaaca agccagacag
     9241 cgaaatgctg ctgcttgatg tgatcccact gtcgctgggc ctcgaaacga tgggcggcct
     9301 ggtggagaaa gtgattccgc gtaataccac tattccggtg gcccgcgctc aggatttcac
     9361 cacctttaaa gatggtcaga cggcgatgtc tatccatgta atgcagggtg agcgcgaact
     9421 ggtgcaggac tgccgctcac tggcgcgttt tgcgctgcgt ggtattccgg cgctaccggc
     9481 tggtggtgcg catattcgcg tgacgttcca ggtcgatgcc gacggtcttt tgagcgtgac
     9541 ggcgatggag aaatccaccg gcgttgaggc gtctattcag gtcaaaccgt cttacggtct
     9601 gaccgatagc gaaatcgctt cgatgatcaa agactcaatg agctatgccg agcaggacgt
     9661 aaaagcccga atgctggcag aacaaaaagt agaagcggcg cgtgtgctgg aaagtctgca
     9721 cggcgcgctg gctgctgatg ccgcgctgtt aagcgccgca gaacgtcagg tcattgacga
     9781 tgctgccgct cacctgagtg aagtggcgca gggcgatgac gttgacgcca tcgaacaagc
     9841 gattaaaaac gtagacaaac aaacccagga tttcgccgct cgccgcatgg accagtcggt
     9901 tcgtcgtgcg ctgaaaggcc attccgtgga cgaggtttaa tatgccaaag attgttattt
     9961 tgcctcatca ggatctctgc cctgatggcg ctgttctgga agctaatagc ggtgaaacca
    10021 ttctcgacgc agctctgcgt aacggtatcg agattgaaca cgcctgtgaa aaatcctgtg
    10081 cttgcaccac ctgccactgc atcgttcgtg aaggttttga ctcgctgccg gaaagctcag
    10141 agcaggaaga cgacatgctg gacaaagcct ggggactgga gccggaaagc cgtttaagct
    10201 gccaggcgcg cgtcaccgac gaagatttag tggtcgaaat cccgcgttac actatcaacc
    10261 atgcgcgtga gcattaacag aggttagtat gggacttaaa tggaccgata gccgcgaaat
    10321 tggcgaagca ctgtacgatg cgtatcccga tcttgatccg aaaacggttc gattcaccga
    10381 tatgcatcag tggatttgcg atctggaaga tttcgacgac gacccgcagg catccaacga
    10441 gaaaatcctc gaagcgattt tgttagtctg gctggacgag gccgaataat ttccgcgtca
    10501 tgcttcacgc cgcagatgcg ttggctgcgc tcgttcaccc cagtcacata gttatctatg
    10561 ctcctggggc ttcactcact tgccgccttc ctgcaacgcg aatcatttag cggaaaaatc
    10621 ctggggctgc caactggcgg cccttttaca aagaaggata actaaaatga cagaagcgat
    10681 gaagattacc ctctctaccc aacctgccga cgcgcgctgg ggagaaaaag caacttacag
    10741 cattaataat gacggcatta ccctgcattt gaacggggca gacgatctgg ggctgatcca
    10801 gcgtgcggcg cgcaagattg acggtctggg catcaagcat gttcagttaa gcggtgaagg
    10861 ctgggatgcg gatcgctgct gggcattctg gcaaggttac aaagccccga aaggcacgcg
    10921 taaagtggtg tggccggatc tggacgatgc ccagcgccag gaactggata accgcctgat
    10981 gatcatcgac tgggtgcgtg acaccatcaa cgcaccggca gaagaattgg gaccatcgca
    11041 actggcacag cgtgctgttg atctgatcag caacgtcgcg ggcgatcgtg tgacttatcg
    11101 gatcaccaaa ggcgaagatc tgcgtgagca aggttatatg gggctgcaca cagtcggacg
    11161 cggttcagaa cgttctccgg tattgctggc gctggattac aacccaactg gcgataaaga
    11221 agcaccggtg tacgcgtgcc tggttggtaa aggtatcacc tttgattccg gcggctacag
    11281 catcaaacag actgcgttta tggactcgat gaagtcggac atgggcggcg cggcaacggt
    11341 taccggggcg ctggcatttg ccattactcg tggattgaac aagcgcgtga agctgttcct
    11401 ctgctgtgcg gataacctga tcagcggcaa tgcgttcaag ctgggcgata tcatcaccta
    11461 tcgtaacggt aaaaaagtcg aagtgatgaa cactgatgct gaagggcgtc tggtgcttgc
    11521 cgatggcctg attgatgcca gtgcgcagaa accggaactg atcattgatg cggcgaccct
    11581 caccggggcg gcgaaaaccg cgctgggtaa tgattatcac gcgctgttca gtttcgacga
    11641 tgcgcttgcc gctcgcttgc tggcgagtgc ctcacaagag aatgaaccgt tctggcgtct
    11701 gccgctggcg gagttccacc gcagccagct gccgtctaac tttgccgaac tgaacaatac
    11761 cggaagcgcg gcgtatccgg caggtgcaag cacggcggcg ggcttcctgt cgcactttgt
    11821 tgagaactat cagcaaggct ggctgcatat cgactgctcg gcgacttacc gtaaagcgcc
    11881 ggttgaacag tggtctgcgg gtgctacggg acttggtgtg cgcacgatag ctaatctgtt
    11941 aacggcgtaa actaatatgc cggatgcggc gtaaacgcct tatcctgcct acaaagtatt
    12001 gcaaattcaa caaa
//
LOCUS       DAEVZA010000146        11690 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEVZA010000146 DAEVZA010000000
VERSION     DAEVZA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.146"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(1..189)
                     /locus_tag="LAF92_004178"
                     /pseudo
     CDS             complement(1..189)
                     /locus_tag="LAF92_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(951..1520)
                     /locus_tag="LAF92_004179"
     CDS             complement(951..1520)
                     /locus_tag="LAF92_004179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793101.1"
                     /translation="MSGNIGANPVPKLPLLAEWVDKNNDDLFVKAKSEKKERTVKKLK
                     NRGLATTLARINTWIDYDDDDNYKWVTEYYLSPVSIESLLTCSYNLITVQAKSKHPNI
                     NSTKLYILPFLSKTRLVILSCMAFYKPSSWDSEVLIDNNIKWTPHIIELITSNDIKKH
                     LTDLAKNFENETLYPILKAFKLLPDSDKK"
     gene            complement(1505..2733)
                     /locus_tag="LAF92_004180"
     CDS             complement(join(1505..2418,2418..2733))
                     /locus_tag="LAF92_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI9793102.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVLRAQLYVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRIYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCLEI"
     gene            2797..3591
                     /locus_tag="LAF92_004181"
     CDS             2797..3591
                     /locus_tag="LAF92_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI9793103.1"
                     /translation="MSGDSGGQSILPLEMLEMLEILRRIEKRGSLEKLRKVRQYCNQI
                     FRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRKLAEYHGSPVTRMAT
                     NLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVPLSRQATDILLQLKT
                     FSEQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRHTMSTILHEQGFNSA
                     WIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSQES"
     gene            3749..4759
                     /locus_tag="LAF92_004182"
     CDS             3749..4759
                     /locus_tag="LAF92_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBI9793104.1"
                     /translation="MSLKAIATALGVSVTTVSRALGGYADVSASTRARVEAEARRRGY
                     RPNTQARRLKTGKTDAVGLVYPGRDVPFNSGVFMDMVSCISRELAHHDIDLLLIADDE
                     HADCHSYMRLVESRRVDALIIAHTLDDDPRITHLHKAGIPFLALGRVPSGLPCAWFDF
                     DNHAGTGQATQKLIALGHKRIALLSEDTSHTYVIARRQGWLDALNEHELEDTWLRLVS
                     PTRRAGYQAVMELMSLPIPPTAIITDNDLSGDGAAMALQLSGRLSGKAAVSLVVYDGL
                     PQDSIIELDVAAVIQSTRSLVGRQISEMIYQIITDSSSKPLQVVWNPVFYPGKTIHSP
                     CS"
     gene            4855..6981
                     /locus_tag="LAF92_004183"
     CDS             4855..6981
                     /locus_tag="LAF92_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBI9793105.1"
                     /translation="MVSKYCRLSSPRSDLIIKTHPHAEIIWWGSALKHFSPDDCASLE
                     RPVANGRLDIDTPLTLMAENALGLFSSPGLEGHRNGLDSSPVFYTVDVEHTENTLRLT
                     SEDSVAGLRLVSELVMTPSGILKVRHALTNLREGDWQINRFAITLPLAERAEEVMAFH
                     GRWTREFQPHRVRLTHDAFVLENRRGRTSHEHFPALIVGTPGFSEQQGEVWAVHLGWS
                     GNHRMRCEAKTDGRRYVQAEALWMPGEKALRKNETLYTPWLYACHSADGLNGMSQQYH
                     RFLRDEIIRFPEQKPRPVHLNTWEGIYFNHNPDYIMQMAERAAALGVERFIIDDGWFK
                     GRNDDRAALGDWYTDEQKYPNGLMPVIKHVKSLGMEFGIWVEPEMINPDSDLFRLHPD
                     WVLSMPGYSQPTGRYQYVLNLNIPEAFAYIYERFSWLLGEHPVDYVKWDMNRELVQAG
                     HEGRAAADAQTRQFYRLLDLLRERFPHVEFESCASGGGRIDFEVLKRTHRFWASDNND
                     ALERCTIQRGMSYFFPPEVMGAHIGHRRCHATFRQHSIAFRGLTALFGHMGLELDPVA
                     ADAKESDGYRRYALLYKEWRQLIHTGVLWRVDMPDPSIQVQGVVSPDQSQALFMISQL
                     AMPDYTLPGILRFPGLAAEVRYRLRVIDHPDLQVVGEGGHTMRKLPVWMNQSLEASGE
                     WLAQGGIQLPVLDPESAILIALERAV"
     gene            7044..8321
                     /locus_tag="LAF92_004184"
     CDS             7044..8321
                     /locus_tag="LAF92_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9793106.1"
                     /translation="MNSASTHKNPDFWIFGLFFFLYFFIMATCFPFLPVWLSDVVGLS
                     KTDTGIVFSCLSLFAISFQPLLGVISDRLGLKKNLIWSISLLLVFFAPFFLYVFAPLL
                     RFNIWAGALTGGVFIGFVFSAGAGAIEAYIERVSRSRGFEYGKARMFGCLGWALCATM
                     AGMLFNVDPSLVFWMGSGGALLLLLLLFLARPSTSQTAMVMNTLGANSSLISTRMVFS
                     LFRMRQMWMFVLYTIGVACVYDVFDQQFATFFRSFFDTPQAGIKAFGFATTAGEICNA
                     IIMFCTPWIIHRIGAKNTLLVAGGIMTIRITGSAFATTATEVVILKMLHALEVPFLLV
                     GAFKYITAVFDTRLSATVYLIGFQFSKQLAAILLSTFAGHLYDRMGFQNTYFVLGMIA
                     LTVTVISAFTLSSSRGSVHPSVEKAPAAHSEIN"
     gene            8318..9751
                     /locus_tag="LAF92_004185"
     CDS             8318..9751
                     /locus_tag="LAF92_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBI9793107.1"
                     /translation="MMKQRLALAQSALEKLCARRGNAWYPIFHLAPPAGWMNDPNGLI
                     YFNGRYHAFFQHHPASAYQGPMHWGHATSTDMLHWQHEPVALAPGDKYDRDGCFSGSA
                     VDDDGVLSLIYTGHICLEDRGNDSIIREVQCLATSHDGIHFEKQGCVLTPPEGIMHFR
                     DPKVWHEEGSWWMVIGARDASDNGQVLLYRGTSLRDWHLEHVLAHSAAGESYMWECPD
                     FFRCGNFHWLMFSPQGMTPSGYRFRNLFQSGVLAGSWKPGSVFALKGGFEELDYGHDF
                     YAPQSMLAEDGRRIIMAWMNMWDSPVPTRSEAWAGCLTLPREVFERDGRLCQRPVREV
                     ESLRRKCQPLSPVRLQGLQLLTENVQAAELLVTWHTVDSHAEHYGVRLGDGLRLYVDN
                     QAGRLVLWRYYPEEGLDGYRSVELPDTEYLTLRIFLDRSSVEVFVNDGEATLSSRIYP
                     QADSRQLSLYAAHGDAILTNGTLWMLT"
     gene            9946..11340
                     /locus_tag="LAF92_004186"
     CDS             9946..11340
                     /locus_tag="LAF92_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBI9793108.1"
                     /translation="MNKLLLGLSVFLLLEYNACAAKTGGLTLEQRMALLEERLEAAEK
                     RAEKAERMLKSFDTEQHSEIRQISSEQNKKAANSYAVVESTKEKTSSPGFLLSGYNDL
                     KFYGDVEFNIDAASKPGQLVMISSGANSESVNERWDLNGRILLGFDGTRKLDNGYFAG
                     FSAQPLADMHGSVNIDDALFFFGKDDEWKVKVGRFEAYDMFPLNQDTFIEYSGNTAND
                     IYADGRGYIYMMKEGRGRSDAGGNFLISKQLDNWYFELNTLLEDGTSLYNNGNYHGRY
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVKNAYGYTDNKGNFVDQSNRSGYGIS
                     MTWNDLKTDPENGIVVTLNTAYLDASNEKDFTASINALWKRFELGYIYAHNKIDEFSG
                     VVCNNGCWIDGEGIYNIHTIHASYQFANVMDMENFNIYLGAYYSILDSNCRYSNCDIT
                     DDRYGARLRFKYFF"
     gene            complement(11586..>11690)
                     /locus_tag="LAF92_004187"
     CDS             complement(11586..>11690)
                     /locus_tag="LAF92_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBI9793109.1"
                     /translation="YLCTLGDTPGQIFRSMVDGYHRHCTAPRGDKPTV"
BASE COUNT         2830 a         2646 c         2907 g         3307 t
ORIGIN      
        1 cgctacggtc cgatgcgtga aaaccccggg cttgcagcga ccgaacggtc gcgccgcctc
       61 tgtcgcgccg tggcctgcgc tcctggcatc ccggcaaaag gccgccggga tgtgaggggc
      121 aacgccgttt cgcgcacgcg ctcttcgtcg ttgcggcggg tgcctgcccg ttgttgtgtc
      181 cggtgccata cacctgttta agcccctgtc gccgggggct gatacagcca caacctgccg
      241 cactgactgt ccagggtaaa tgaacagccc acaaaatttt ttacccaaaa cccggtaaaa
      301 aattttgcgt tctgcccagg acaggtgcgt caggccgtgg cagtgatgcc ccttgcggcg
      361 agggttctca aacaggtgat ggtgaatacc agagggaaca aaaggcaggt ccccgcaaaa
      421 cgtcaaaact gcggctcccg aaactcaaca gctaccgtgt tctgtcaaga aaaaatcgct
      481 taaattcagt cgtacagccc ttaagtactg ccagcaggct tgtcagtacc aagtttccga
      541 tactgcgcgg ctacgctaac ggggttaaaa gggcttactt atactacatc atcgctgaaa
      601 gcttgtatgg tttcgcggaa cagggagtaa caggagaata ttcactctct accggctaac
      661 agttagctta attgcagcaa ctggtaacca actgctagaa aaattcacca tgaataataa
      721 ttgtgctgat tacctgcaat tattgtagtg aaatttctgt tcatattttt tcgctagcga
      781 tgtcttgttt gagaaaaaac actatttgtt gaatagtttg attctggtat ttggagtctt
      841 ttaaaggggg attctatatg gatatcactt tttatatgtt gtaaataatg ccgctgtaga
      901 agaacaacgg caaatgaagg ggggtattgt tacaataatc ttatttgtct ctatttttta
      961 tccgaatcag gtagcaattt aaaagctttc aatatggggt aaagtgtttc attctcaaag
     1021 ttttttgcta aatcagtgag gtgttttttg atgtcattgc tggtaatgag ctcaattatg
     1081 tgaggagtcc atttgatatt attgtcaatc aacacttcag agtcccaact agatggttta
     1141 taaaaagcca tgcaagataa aataactagt ctcgtcttcg ataagaatgg taatatatat
     1201 agtttggtac tgtttatgtt agggtgtttt gattttgcct gcacagtaat taagttatat
     1261 gagcaagtta gtaatgactc aatactaaca ggcgataagt aatattctgt aacccacttg
     1321 taattgtcat catcatcata atcaatccac gtatttatcc tggctaaagt tgttgctagg
     1381 cctctgtttt ttagtttctt tacagtcctt tcctttttct cagatttggc ttttacgaat
     1441 aaatcatcat tatttttatc aacccactca gccaggagag gcaatttagg aactggattt
     1501 gcccctatat ttccagacac ctgttatcac ttaacccatt actggcctgc tgccgcagat
     1561 attcccgtgg cgagcgataa cccagtgcac tatgcggatg ccattcgtta taatgctcga
     1621 acgcctctgc aaggttcttt gctgccgtta acccgtctgg tttaggcatg atactgatgt
     1681 agtcacgctt tatcgttttc acgaagctct ctgctatgcc gttactctcc ggactccgca
     1741 ccgccgtgtt cttcggttca agccccaaca tccgggcaaa ctgccgtgtt tcattagccc
     1801 ggtagcatga accattatcc gtcagccact ctactggaga cgccggaagc tcgttgccga
     1861 agcggcgttc caccgctccc agcatgacgt cctgtactgt ttcactgttg aagccgcccg
     1921 tagtgaccgc ccagtgcagt gcctcacggt cacagcagtc cagcgcgaac gtgactcgca
     1981 gtttttctcc gttatcacag cggaactcga acccgtcaga gcaccatcgc tgattacttt
     2041 ctttcacagc cactctgccg gtatgtgccc gtttcgatgg cggtacagcg ggttttcgct
     2101 caagcaacag cgcattctgg cgcatgatcc ggtaaatacg tttggcattg atcgcaggca
     2161 taccatcaag ttcggcctgt ctgcgaagca gcgcccatac ccgacgataa ccatacgtgg
     2221 gcagctctcc gataacatgg tgtatacgga gaagcacatc cgtatcatca gtgtgacgac
     2281 tgcggcggcc atccatccag tcatcggttc gtctgagaat gacgtacaac tgcgcacgca
     2341 acacccggag acaacggctg actaagctta ctccccatcc ccgggcaata agggcgcgtg
     2401 cgctatccac ttttttgcac gcccgtattc aacggcttct ttaaggagtt cattttccat
     2461 cgtttttttg ccgagcaggc gctggagttc tttaatctgc ttcatggcag cagcaagttc
     2521 agaggcagga acgacctgct ctcctgcggc cacagcagta agacttccct cctggtattg
     2581 cttgcgccag agaaataact ggctggctgc cacaccgtgt tgccgggcaa caagggagac
     2641 cgtcattccc ggttcaaaac tctgctgaac aattgcgatc ttttcctgtg tagtacgccg
     2701 tctgcgtttc tccggtccta agacatcaat catctgctct ccaatgacta gtctaaaaac
     2761 tagtattaag actatcactt atttaagtga tattggttgt ctggagattc agggggccag
     2821 tctatcctcc cgctggaaat gctggaaatg ctggaaattc tgcgccgcat agaaaaacgt
     2881 ggctcgctgg aaaaactgcg taaggtccgc caatactgta atcagatttt tcgttatgct
     2941 atcgccaccg gacgagccac tgtcaatccg gcatctgaac tgaccagtac gctggcggca
     3001 ccaaaagctg cgcatttccc ccacctgaga gcagatgagc tccctgtttt tctccggaag
     3061 ctcgctgaat atcatggcag tcctgttacc cgcatggcga caaatcttct gcttctgacg
     3121 ggtctcagaa ccattgagct acggtccgct gaatggtcag aaattgattt tgataatgcc
     3181 ctgtggacaa tccctgaaag ccgcatgaaa atgcgacgta aacatgtcgt accactgtca
     3241 cgacaggcca ctgacattct gctgcagctc aaaactttct ccgagcaata ccgactggtt
     3301 tttccgggac gttgtgatat caacaagcca atgagcgaag ccagcatcaa tatggtgctc
     3361 aaacgtatcg gttacgatgg cagggcaacc ggtcatggtt ttcgtcacac catgagtacc
     3421 attctgcacg aacagggctt taattctgcc tggattgaaa tgcagttagc tcatgtggat
     3481 aaaaattcca tcaggggtac ctataatcat gccctgtatc tcgatggtcg ccgtgaaatg
     3541 atgcaatggt acgctgacta cattgattca ctttccagcc aggagagtta attacacttt
     3601 gtcctgaaat tattctcaca atcaataacc tttccctctg atcttacaga tcagagggat
     3661 tccgttaact tacaataagt gatattggtt gtctggagat tcagggggcc agtctaacgg
     3721 tctactataa taattagcag gagataatat gtcacttaag gcgattgcca ctgcactcgg
     3781 agtttctgtc accactgtca gtcgggctct tggaggctat gcagatgtgt cagcttccac
     3841 ccgtgcacgg gtggaagctg aagctcgtcg acgcggctac cgtcctaata ctcaggcaag
     3901 aagactcaaa accggtaaaa ccgatgctgt cggtctggtt tatcctggac gtgatgtgcc
     3961 atttaacagt ggtgttttta tggatatggt cagttgcatc agccgggaac ttgctcacca
     4021 tgatattgac ttactactga tagctgatga tgagcatgcc gattgccaca gctatatgcg
     4081 gcttgttgaa agtcgcaggg ttgatgctct catcattgca cacactctgg atgacgatcc
     4141 ccgtatcaca caccttcata aagcaggtat tccatttctg gctcttggtc gggtaccatc
     4201 tggtctgccc tgtgcgtggt ttgactttga taatcatgcc gggactggac aggcaaccca
     4261 gaaactgatt gctttgggac ataaacgtat tgcattgttg agtgaagaca cttcacatac
     4321 ctatgttatt gcaagacgtc agggatggct tgatgcactg aatgagcatg aactggaaga
     4381 tacatggctg cggctggttt ctcccacacg gcgagcagga tatcaggccg tgatggagtt
     4441 aatgtcacta ccgataccac caacagctat tattactgac aatgatctga gtggagatgg
     4501 tgctgccatg gcgctgcagt tgagcggtcg tctttcagga aaagctgcgg tatctctggt
     4561 tgtatatgat ggtttgccac aggacagtat tattgagctg gatgtagctg cagttattca
     4621 gtcaacacga agcctcgttg gtcgccagat ttccgagatg atatatcaga taattactga
     4681 ttcatcatca aaaccactac aggttgtctg gaacccggta ttttatccgg gaaagacgat
     4741 tcattctcct tgctcctgat ttttttatcc agctcacata tccgaaacgt tttggttgat
     4801 acccgaaacg tttcggatca acagtaaggc atccctgaga aaggagaatg tcttatggtt
     4861 tcaaagtact gcagactgag cagtcctcgc tctgatttaa ttattaaaac ccatccacat
     4921 gcagaaatta tctggtgggg ctctgcactg aaacatttct caccggatga ctgtgccagc
     4981 ctggaaagac cggttgcgaa tggtcgtctg gatattgata cgccactgac actgatggct
     5041 gaaaatgccc tgggactttt tagctctccg ggactggaag gacacaggaa tgggctggat
     5101 tcatctcctg ttttttatac agttgacgtg gaacataccg aaaacactct gagacttacc
     5161 agtgaagatt cggtagccgg cctgcgtctg gtcagcgagc tggtgatgac gccatcagga
     5221 attctgaaag ttcgtcatgc actgaccaac ctcagagagg gggactggca gataaatcgt
     5281 ttcgccatca ctttaccttt agcggagcgt gcggaagaag tcatggcttt tcacggacgc
     5341 tggactcgtg aatttcagcc gcacagggta cgtctcactc atgatgcttt tgttctggaa
     5401 aatcgcagag ggcggacatc tcatgagcat tttccggcgc tgattgtcgg cacgccaggc
     5461 ttctcggaac aacagggaga ggtatgggct gtgcatctgg ggtggagtgg aaatcaccgt
     5521 atgagatgtg aggcaaaaac tgacggcagg cgttacgttc aggctgaggc tctgtggatg
     5581 ccgggtgaga aggctctcag gaagaatgaa accctgtaca ccccgtggct atatgcctgc
     5641 cactctgcgg atggcctgaa tggaatgagt cagcaatacc atcgtttttt gcgtgatgaa
     5701 attatccgtt tccctgagca aaaaccccgc cccgtacatc tcaatacctg ggaaggtatt
     5761 tatttcaatc acaatcctga ttacatcatg cagatggctg agcgtgcagc agcactgggc
     5821 gttgaacgtt tcattattga tgatggctgg tttaaaggac gtaacgatga ccgcgcggct
     5881 ctgggcgact ggtataccga tgaacagaaa tacccgaacg ggctgatgcc ggttattaaa
     5941 catgtgaaat ctctcggtat ggaatttggc atatgggttg agccggaaat gattaacccg
     6001 gattctgacc tgtttcgtct tcatcctgac tgggtattgt caatgcctgg atattcccag
     6061 ccaaccggaa gatatcagta tgttcttaac ctgaatattc ctgaggcctt tgcttacatt
     6121 tatgaacgtt tctcatggtt actgggagaa catccggttg attatgtgaa atgggacatg
     6181 aatcgtgagc ttgtacaggc agggcatgaa ggccgtgcgg cagcagatgc acagacccgt
     6241 cagttctatc gattgcttga tctcctccgt gaacgttttc cacatgttga gtttgagtcc
     6301 tgtgcttccg gtggggggcg tattgacttc gaagtcctga aacgcacaca ccggttctgg
     6361 gcatctgaca ataatgatgc cctggagcgc tgcaccatac aacgtggcat gagttacttt
     6421 ttccctcctg aggtgatggg ggcacatatt ggccatcgcc gctgccatgc aactttccga
     6481 cagcacagca tcgcttttcg tgggctgaca gcattgttcg gccatatggg gctggagctg
     6541 gatccggtgg ccgcagatgc gaaggaatct gacggttatc gccggtatgc cttgctctat
     6601 aaagaatggc gacaactgat tcatacaggt gttctctggc gtgtggatat gccagatcct
     6661 tcgatacagg ttcagggagt cgtcagccct gatcagtctc aggcactttt tatgatcagc
     6721 cagcttgcaa tgccggatta caccttacca ggcatacttc gttttcccgg tctggcggcg
     6781 gaagtgcgtt accggcttcg ggttattgat cacccggacc tccaggtggt cggtgaaggc
     6841 ggtcatacca tgcgcaaatt acctgtctgg atgaatcaga gccttgaggc cagtggtgaa
     6901 tggctggcac agggagggat tcagctcccc gtactggatc cggagagtgc gattttgata
     6961 gcacttgaaa gagctgtgtg aagagaggta cggagcctca gctccgtcct gtttctcaat
     7021 attatcccgt gagggtaaga ataatgaatt ctgcaagtac gcataaaaat cccgatttct
     7081 ggattttcgg tctgttcttc tttctctact ttttcatcat ggcaacctgt tttccgtttt
     7141 tgcctgtatg gctgtccgat gtggtcgggc tgagtaaaac ggatacaggt atagtctttt
     7201 catgtctttc tctgtttgcc atcagtttcc agccattgct tggggtcata tcagatcgcc
     7261 tgggactgaa aaaaaacctg atctggagta tcagcctgtt actggtattt ttcgccccct
     7321 tttttttata tgtatttgcg cccctgctgc gcttcaatat ctgggcaggc gcactgactg
     7381 gcggtgtttt tattggtttt gtgttttctg caggtgccgg agctattgaa gcttatatag
     7441 agcgggtcag tcgcagtcgt ggatttgaat acggtaaagc gaggatgttc gggtgtctgg
     7501 gctgggcgtt atgtgcgact atggctggaa tgctttttaa tgtcgatcct tctctggttt
     7561 tctggatggg gtcaggaggc gcattattgt tgcttcttct gttgtttctg gcgcgcccca
     7621 gtaccagcca gacggcaatg gttatgaata cactgggtgc caattcttcc ctgatttcga
     7681 ccagaatggt cttcagcctg tttcgcatgc gtcagatgtg gatgtttgtt ctctacacga
     7741 ttggtgtggc ctgtgtctat gatgtatttg atcagcagtt tgccacattt tttcgttcat
     7801 tctttgacac tcctcaggca ggaataaaag cattcggatt tgctaccact gcgggggaga
     7861 tttgtaatgc cattatcatg ttctgtacac catggataat tcatcgcatt ggtgccaaaa
     7921 ataccctgct tgttgcgggg ggaattatga ccatccgcat taccggttct gcttttgcca
     7981 ccaccgcgac agaagtggtg attctgaaaa tgcttcacgc tcttgaagtt ccatttttgc
     8041 tggttggggc gttcaaatat attacggcag tgtttgacac ccgactgtca gcgaccgttt
     8101 atttaatagg ttttcagttt tccaaacaac ttgctgcaat acttctctct acctttgccg
     8161 gccacctgta tgatcgtatg ggattccaga atacgtattt tgtgctcggg atgattgctc
     8221 tgactgttac cgtgatatca gctttcacgc tgagttcttc ccgcgggagc gtacaccctt
     8281 ctgtagaaaa agcccctgca gcgcattcgg agattaactg atgaaacagc gtcttgcttt
     8341 ggcacagtcc gcccttgaaa aactttgcgc acgtcgtggt aatgcctggt acccgatttt
     8401 tcatctggct ccacctgccg gctggatgaa tgatccaaat ggccttattt acttcaatgg
     8461 gcgttaccat gcgttcttcc agcatcatcc tgcaagcgca tatcaggggc caatgcactg
     8521 ggggcatgcc accagtaccg acatgttgca ctggcaacac gaacctgtcg cgctggcacc
     8581 cggagataaa tatgatcgtg atggctgttt ttcagggagt gccgtggatg atgatggcgt
     8641 gctatcactt atttatactg gtcatatttg tctcgaagat cgtggtaatg acagcattat
     8701 ccgtgaagta cagtgtctgg ctaccagtca tgatggtatt cactttgaga agcagggctg
     8761 tgtgctgaca cctccggaag gtatcatgca tttccgtgat cccaaagtct ggcacgaaga
     8821 aggctcctgg tggatggtca ttggtgcccg ggacgcttct gacaatgggc aagttctgtt
     8881 gtatcgcggg acatctttgc gggactggca tctggagcat gttctcgctc attccgcagc
     8941 cggagaaagt tatatgtggg aatgccccga tttcttcagg tgtggtaatt ttcactggct
     9001 gatgttctca ccacagggga tgaccccttc cggttatcgg ttccgtaacc tttttcagag
     9061 cggtgtgttg gcagggagct ggaagcctgg ttctgtcttt gcgctgaaag ggggatttga
     9121 agagctggat tatggtcatg acttttatgc tccacagtcc atgctggctg aggacggcag
     9181 gcgtatcatt atggcatgga tgaatatgtg ggattcaccc gtgcccaccc gcagtgaagc
     9241 ctgggcagga tgtctgacgc tgcccagaga ggtttttgag cgcgatggcc ggctgtgcca
     9301 gcgacctgtg cgtgaagtcg aatctctgcg cagaaaatgc cagccattat cccctgtaag
     9361 gttacagggt ttgcaattac tgaccgaaaa tgtacaggcc gcagaattat tggtgacgtg
     9421 gcatacggtt gacagtcatg cggagcacta tggcgtccgc cttggagacg gtctgcgact
     9481 ttatgtggat aatcaggccg gacgactggt actgtggcgc tattaccctg aggaagggct
     9541 ggatggttac cgcagtgttg aacttcctga tacagaatat ctgactcttc gtattttcct
     9601 ggatcgttca tctgttgaag tgtttgttaa cgatggtgag gcaaccttat caagtcgtat
     9661 ttatccgcaa gcggactcga gacaattatc gttatatgcc gctcatggcg atgcgatatt
     9721 aactaatggc actttatgga tgctgacctg agatatatcg atatcagtat tttttttgat
     9781 attatctgtc attactttgc cagtacacat taattatctt tgctgtgtgc ttttggtaat
     9841 gtgttgcgat tatatatacc agtctggtga agagtatgaa tggtattttg tgttgataca
     9901 gagaatataa gagtacccac atttgttttt atttaagggg gtaaaatgaa taagttactc
     9961 ttgggtttgt ctgttttttt attactggaa tacaatgcgt gtgcagcaaa aacagggggg
    10021 ctaacgcttg aacaaagaat ggcattgctg gaagaacgcc tggaagctgc agaaaaaagg
    10081 gcggaaaaag cagaaagaat gttaaaaagt tttgatacag aacaacattc tgaaatacgt
    10141 caaattagtt cagagcagaa taaaaaagct gctaacagct atgcagtagt tgagtcaaca
    10201 aaagaaaaaa catcttctcc aggttttctg ctctctggtt acaatgattt gaagttttat
    10261 ggtgatgtgg agtttaatat agatgctgcc agtaaacccg gtcagttagt aatgataagt
    10321 tccggggcga acagtgagtc agtgaatgaa cggtgggacc ttaatggccg tattctatta
    10381 ggttttgacg gtacccgtaa gcttgataat ggttatttcg ctggattttc agcacaacca
    10441 ctggcggata tgcacggttc agtgaatatt gatgatgcat tattcttttt tggaaaagat
    10501 gacgagtgga aagtgaaagt cggtcgtttt gaagcttacg atatgttccc tctaaatcag
    10561 gatactttca ttgagtattc cggtaataca gctaacgata tttatgctga tggccgtggt
    10621 tatatctata tgatgaaaga gggacgcggt cgttctgacg ctggtggtaa tttcctcatc
    10681 agtaaacaac tcgataactg gtattttgag ttaaacacgt tacttgaaga cggaacatct
    10741 ttatataata acggtaatta tcatggacga tatatggagc agcagaaaaa tgtcgcttat
    10801 ctgcgcccgg taattgcctg gtcgccaacg gaagaattca cagtctccgc agcgatggaa
    10861 gcaaacgtgg taaaaaatgc ttatggctat accgataata aggggaattt tgtcgatcag
    10921 tccaatcgtt ccggttatgg tattagcatg acatggaacg atctgaaaac agatccggaa
    10981 aatggcatcg tggttactct taataccgcc tatttggatg ccagtaatga gaaagatttc
    11041 actgccagta ttaatgctct gtggaaacgt ttcgaactgg gttatatcta cgcacataat
    11101 aagattgatg aatttagtgg tgtcgtgtgt aataatggtt gctggattga tggtgaagga
    11161 atatacaaca ttcacaccat tcatgcatct tatcagtttg ctaatgtgat ggatatggag
    11221 aactttaata tttacctcgg ggcttattac tcaattctgg atagtaactg tagatatagt
    11281 aattgtgaca ttaccgatga tcgttacggt gcccgattac gtttcaaata ctttttttga
    11341 tgcatagtaa caaagtattg ttccacactt acattaacgc gtttttcggg tacaccatcg
    11401 gtgagcatac gaataatcgc tgtctgtcga tggttgattt aatttattgc gcaacgagcc
    11461 agctaccggg aactggctct gaagttgcgt caggtcacga agtactgccc ggggaataat
    11521 gccagactaa ctctgaccag tgccacttga cgtctccgcc agcgcagcgg aggcaaatag
    11581 tactgtcaga ccgtgggctt atccccgcgc ggagcggtac aatgacggtg ataaccgtca
    11641 accatactgc gaaatatttg ccccggcgtg tcacccagag tgcacagata
//
LOCUS       DAEVZA010000147        11470 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEVZA010000147 DAEVZA010000000
VERSION     DAEVZA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.147"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..195)
                     /gene="rrrD"
                     /locus_tag="LAF92_004188"
     CDS             complement(<1..195)
                     /gene="rrrD"
                     /locus_tag="LAF92_004188"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBI9793110.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKT"
     gene            complement(195..410)
                     /gene="essD"
                     /locus_tag="LAF92_004189"
     CDS             complement(195..410)
                     /gene="essD"
                     /locus_tag="LAF92_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBI9793111.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            999..2096
                     /gene="ompC"
                     /locus_tag="LAF92_004190"
     CDS             999..2096
                     /gene="ompC"
                     /locus_tag="LAF92_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBI9793112.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGANYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(2285..2668)
                     /gene="quuD"
                     /locus_tag="LAF92_004191"
     CDS             complement(2285..2668)
                     /gene="quuD"
                     /locus_tag="LAF92_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HBI9793113.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2754..2894)
                     /locus_tag="LAF92_004192"
     CDS             complement(2754..2894)
                     /locus_tag="LAF92_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBI9793114.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2891..3253)
                     /gene="rusA"
                     /locus_tag="LAF92_004193"
     CDS             complement(2891..3253)
                     /gene="rusA"
                     /locus_tag="LAF92_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HBI9793115.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(3250..3540)
                     /locus_tag="LAF92_004194"
     CDS             complement(3250..3540)
                     /locus_tag="LAF92_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBI9793116.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(3533..3703)
                     /locus_tag="LAF92_004195"
     CDS             complement(3533..3703)
                     /locus_tag="LAF92_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793117.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3703..4158)
                     /gene="ybcN"
                     /locus_tag="LAF92_004196"
     CDS             complement(3703..4158)
                     /gene="ybcN"
                     /locus_tag="LAF92_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HBI9793118.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGFETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(4155..4256)
                     /locus_tag="LAF92_004197"
     CDS             complement(4155..4256)
                     /locus_tag="LAF92_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793119.1"
                     /translation="MRTHNPNSLLPSQMQKCTCDFLHPTFDLCGGEA"
     gene            complement(4369..5001)
                     /locus_tag="LAF92_004198"
     CDS             complement(4369..5001)
                     /locus_tag="LAF92_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793120.1"
                     /translation="MKFSRIEQSPTTDEGNYVKIDHDFGYFLDLNKMASIANYEDESA
                     ALLKLMLIHEDFLRVVIENLRPDGSEKYNSIRSYKYFASKLSAAVLLGLPVSLADAMA
                     ELNTIRNKYAHKIEYVITNEEVDKLAESIKNIKLEEINHGGYVHQVVEPILKDGIYAI
                     ALMKELPPDLEENRIRICKLVTSAHCMCLLGAFWLINTLHVKGKLKLSKA"
     gene            complement(4998..5174)
                     /locus_tag="LAF92_004199"
     CDS             complement(4998..5174)
                     /locus_tag="LAF92_004199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793121.1"
                     /translation="MNSQLPVLREVNIDNNECVVTIAQTGKSTWSASGFSIEHSITQP
                     KASDPRYMKQEEDK"
     gene            complement(5337..6041)
                     /locus_tag="LAF92_004200"
     CDS             complement(5337..6041)
                     /locus_tag="LAF92_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793122.1"
                     /translation="MLAKNSEAPLAATSGASIENNRVGNIDMNIVARSDLNFQGKAIV
                     PVAGMTGIWLTSTEIANALQYKSAKSVTNLFNQNADEFTSGMTQVIESVTSGNYRKKV
                     RVFSLRGAHLIAMFARTPVAKEFRRWVLDILDREIQQSPITKQFTDNELCTLAWLWRA
                     SDVMLTACQNVTPLLQVAEHREAGRFTSIEQEYPQILNRARAILARETAHVKFQPWQD
                     DKWSRVLPYFRQERLQ"
     gene            complement(6031..6189)
                     /locus_tag="LAF92_004201"
     CDS             complement(6031..6189)
                     /locus_tag="LAF92_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBI9793123.1"
                     /translation="MEKKEVKITLRYPQNVKDEFKRIAQEEGMSENAAIVQALVWALK
                     FRERIHAR"
     gene            6290..6430
                     /locus_tag="LAF92_004202"
                     /pseudo
     CDS             6290..6430
                     /locus_tag="LAF92_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
     gene            6635..7114
                     /locus_tag="LAF92_004203"
     CDS             6635..7114
                     /locus_tag="LAF92_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015584732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793124.1"
                     /translation="MSKFDRYLQREILALAIDAYPAYIGHKHSKIPASYELLKNAKED
                     LVYPNIHYLHEHSLINFDKIGYRNNNSNPLAWVKATSHGIDFVQQDGGLSAILNVKTI
                     RFHKDTIVVLEDLIAISNMNDTDKEKAKSILSELPVDAIKTVVQTLTTTALNALLKS"
     gene            complement(7260..7682)
                     /locus_tag="LAF92_004204"
     CDS             complement(7260..7682)
                     /locus_tag="LAF92_004204"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI9793125.1"
                     /translation="MVNFVKFASCNLPDDKKLITLNTYRSSYGWSQKVYEINDVQNIY
                     FIKHQGYLVGYVEIEYMNLDISIPREGYIFLHEIHIAKSMQGKSIGFQVISSLLKKLP
                     VVEMVVVNVNSGMVKLVNKFNVLNCYKGENTSTYRITS"
     gene            complement(8006..8707)
                     /locus_tag="LAF92_004205"
     CDS             complement(8006..8707)
                     /locus_tag="LAF92_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HBI9793126.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREETSVTAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELAHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(8704..9723)
                     /locus_tag="LAF92_004206"
     CDS             complement(8704..9723)
                     /locus_tag="LAF92_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBI9793127.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSDESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLCPAKLRDKWTQLEINRNKQQAGVTAGKSKLDLTNTDWI
                     YGVDL"
     gene            complement(9720..10259)
                     /locus_tag="LAF92_004207"
     CDS             complement(9720..10259)
                     /locus_tag="LAF92_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HBI9793128.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAEDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(10329..10559)
                     /locus_tag="LAF92_004208"
     CDS             complement(10329..10559)
                     /locus_tag="LAF92_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI9793129.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            10664..11353
                     /locus_tag="LAF92_004209"
     CDS             10664..11353
                     /locus_tag="LAF92_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9793130.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
BASE COUNT         3114 a         2630 c         2453 g         3273 t
ORIGIN      
        1 cgttttaccg agcatgatgt cttttcctgt atgcccgtga catacagtcc atacaccaac
       61 aatatctttg tatggtatgt agctgacacc ttccagacca tcgttaccac ttgggccagt
      121 gattaacact gatgctatag caattgctcc gccaccaata gcagcagcaa cggcttttcg
      181 taatgatgga ggcattattc acctctcgca gccttgcgct tatcttcttt aatcttgaaa
      241 taaaggtttg tcaggtacgt cagcaggcca aataccaggc tacccagcac acctattgct
      301 gcccactgtg agggcgtgac tttatcgagc agctgtaaaa accagtaacc ggcactacct
      361 gctgaggtgc cataggcgac acccgttgtt aacttatcca tggatttcat aaccccacct
      421 cgcagacaaa gcgggtgtaa attgagggaa tacaacgtat cgcaaaaaag cagaaacgta
      481 acagactcgg agtcagtgaa taactcaggt attgagttat cagctaatat cgagactcaa
      541 aaaatggaaa aaccagctcg acggcgggtt taagctgtgt gacgaagtaa ccactcttaa
      601 cagcataacc aattttttac gtacgtaaac cactgaatga tatttatgag aatgctaccg
      661 agtgttcaaa acaccaccac aaatacataa gaaaatctca acaaataacc aataaataat
      721 ttccggagtt atttttagtt aatttaaatt aaaccaccga attatagaac ccccataaat
      781 aacagccatt aatataaatt agctaatagg tttatttttg ttcaaataag agccataaat
      841 aggtttcgat agaaaaagtt cagataaaaa tagagatcta cttcacaaat taaatgagaa
      901 actaaaactt acatcttgaa ataatcacat tgattagatg aatatttatc acgcagtgac
      961 atcatttttt aataatagtt caaaaaaaag ggctcacgat gaaaaaatta acagtggcaa
     1021 tttctgctgt agctgcatca gtactgatgg cgatgtctgc tcaggcagct gaaatttata
     1081 ataaagacag taacaagctg gatctgtacg ggaaagttaa tgccaagcac tacttttcct
     1141 ctaacgatgc agatgatggt gatactactt atgttcgtct gggcttcaaa ggcgaaaccc
     1201 aaatcaacga tcagctgact ggtttcggtc agtgggaata tgaattcaaa ggcaaccgcg
     1261 ctgaatctca aggttcctcc aaagacaaaa cccgtcttgc atttgcaggc ctgaaattcg
     1321 gtgactacgg ctcaatcgat tacggccgta actacggtgt agcatacgac atcggtgcgt
     1381 ggactgacgt tctgccagaa ttcggtggcg atacctggac ccaaacagat gtgttcatga
     1441 ctggtcgcac cactggtgtt gcaacctatc gtaacaacga cttctttggt ctggttgatg
     1501 gtctgaactt tgctgctcag taccaaggca aaaacgatcg tagcgatttc gataactaca
     1561 ccgaaggtaa cggtgatggc ttcggtttct ctgctaccta tgaatacgaa gggttcggta
     1621 tcggtgcaac ttatgcgaaa tctgatcgta ccgacactca agttaatgca gggaaagttc
     1681 ttcctgaagt atttgcttcc ggtaaaaatg cagaagtttg ggccgcaggt ctgaaatatg
     1741 acgctaacaa catttacctg gccactacct attctgaaac ccagaatatg actgtatttg
     1801 ctgatcactt cgttgctaat aaagctcaaa acttcgaagc tgttgcacaa tatcagttcg
     1861 atttcggtct gcgtccgtcc gttgcttacc tgcaatctaa aggtaaggat cttggagtat
     1921 ggggcgatca ggacttagtc aaatatgttg atgtaggtgc aaactattac ttcaacaaaa
     1981 atatgtctac tttcgttgat tacaaaatca acctgcttga caaaaatgac ttcactaaag
     2041 cactcggtgt aagcactgat gacatcgttg ctgtaggtct ggtttaccag ttctaatctg
     2101 attacgaaaa agatatgttg gggaggcttt gcctccccaa catataagtg gctccctcaa
     2161 gccacttcct ttaggagcac aaccttgctt ctaactatat aaaccttctg ttatatatta
     2221 ccctttattt ttgggggcgt tgcaacgccc catttttaat aatttttagt aaacaattgg
     2281 catattaatt agagttatta acaacgatat ccatctctaa ccggatatct aatgccatta
     2341 acatcccttc aattatgccc tcagccttct gtaacctttt cccgatataa ccatcagagc
     2401 agcaatgctt acctgccagt gacatgaatg tcataccgac tacataataa tctactaata
     2461 aatcgtgcaa atcgctgttg ttctttttca gacgggccat gcacccgcaa atgatcatcg
     2521 cgtcatcgtc acaacattgc gggcgagatt ttacttttga aggaattaat cccttaaaac
     2581 cggcggcaat ggacgaccag gtcacatctt catgattatt agccgcccac gctccccaac
     2641 gctcaagaac catctgaata tcacgcatca acttactcca caaaaatcag accagaacgc
     2701 caattacaag caaaaatcaa caaaacagta ttagttgatt gttatctctg acttcatact
     2761 cctgctcctg tcagggtttt ggcgtaattc ttcagtattc ggtaatcggt caaaacagaa
     2821 ccggggaaac gatataagcg cagacgcccc cagcggtggc gaagaagttc tgccatatta
     2881 aactcaaaca tcattcattc cccatttcgg tgatggtcag ttccagcctc ccacctttgg
     2941 taacaggcat cttcacaacg cggtaatcaa cgacctgagc atcatccagc cagaaacctg
     3001 ctttagtgag tgcgtcaaaa gcggcttttt gcagattatc caggtcacgg cgacggcgat
     3061 ccggcatgtg gcactcaatg cggattttca caggcatagc caggccgata tccagcattg
     3121 cgtttttaat gattcgggcg acgttatcgc ggtatgcctg cccctctgca ctgacgtgcg
     3181 tgcgcccgcg attatggcgg taatagcgat tattgctcgg aggccatggt aatgtgatac
     3241 tgtaggtatt cacgccttaa taaccccctc tttcagccag ataacctgtg ttctcgccat
     3301 accttccagc gcgcattctt ttgcatatgc agcatcgaca aaatgtgtgc ggcggtcgat
     3361 ttcgtcgtgg caggcagaac atgcaatggt ggcaatcagg tctggcggtt tggtaccggt
     3421 gccgcacaat ccagtcagcc ggatatgtgc cagtacagac gtttcagggt tgccattaca
     3481 tacgccaggg attcttacct ggcattcccg accacgcgct gcttttctca aatcagccat
     3541 gattcctcct tgctgccagt cgcaaccatt ttttatcaac caggctagcg gtatatccga
     3601 gcagtgttgg tatttcggat ggcttcagct caggcttacg cttacgacga tttgatactc
     3661 tgtagatgtg tccgttcatg acacgaataa gcggtgtagc cattacgcct cctgcttgtc
     3721 gcggagcagc tggaactcgc agctctgcgg aatagtcagg tggcagccaa tattcaccgc
     3781 ccaggcttca accttacaca ggaagacata catctctccg gtatcaagat cggaggtatg
     3841 gcgtaatgac tgaatggtgg tgatttcgcc ggttacgaca tcaaccaggt ctttggtttc
     3901 aaaaccgaga taagtgtgtt tgagagcatc ttttacccaa gctgcagtag cgaacgattt
     3961 ccccctgctt atgaggtatt cactgatttc gctgtaccac atgtggctaa gtgcattctg
     4021 ggaaagactg cgtctctcgc gccacggttt aagcaccatg cgaaagcatt ttccgtcctc
     4081 cagataaggc tggatctgct gaccgatagc ggtgaagtta ccgcgatgta atttgatgcc
     4141 gtcttgtggg aggttcacgc ttcacctccg cagaggtcaa acgttggatg caaaaaatcg
     4201 caggtgcatt tctgcatctg tgaagggaga agagagtttg gattgtgtgt gcgcataaac
     4261 gtccccgttt agcgcagaag tcaccggagt tgttcaggtt ccggtgatac aattatggcg
     4321 aattgatgac ggagaatcaa ttttgcgaaa atgacacgat tgatctactc aggccttgga
     4381 aagcttgagc ttacccttta cgtgcagagt gttaattaac cagaaggccc cgagcaaaca
     4441 catacaatgc gcgctagtga ctaacttaca aatacgaatg cgattttctt caagatcagg
     4501 aggtagctct ttcatcaacg ctatagcgta gattccatcc ttgagaattg gctcaacaac
     4561 ttgatgcacg tagccaccat ggttaatctc ttctaacttt atgtttttaa tcgattctgc
     4621 gagtttatca acctcttcat ttgtaattac atattcaatt ttatgagcgt acttgttacg
     4681 tattgtgttg agctcagcca tagcgtctgc aagcgagaca ggaagcccta acagaactgc
     4741 ggcagacagc tttgacgcaa aatacttata gcttcttatc gaattgtatt tttcagagcc
     4801 atcaggtcgt aagttttcaa taacaacccg aagaaagtcc tcatgaatta gcatcaattt
     4861 taatagcgcg gcagactcat cctcatagtt agctatcgag gccattttat taagatctag
     4921 aaaatagcca aagtcatgat cgattttcac gtagttaccc tcatccgttg ttggactttg
     4981 ttcaattcga gagaatttca tttatcttcc tcctgtttca tatatctagg gtcagatgct
     5041 ttaggctggg tgatgctatg ctcaatggag aagccggacg cgctccatgt tgatttgcca
     5101 gtttgagcta tcgtgacaac acattcatta ttgtcgatgt taacttcgcg cagcactggt
     5161 aactgtgaat tcattttcta actcctgttt aatgttgaca ccgcatttat gctgtcacga
     5221 cacaattact ttctatcctg ctgtgatgct gctgacagcg gcatccaata ccttaccttc
     5281 tcagcattct gcaaaatcga tgacgagtca tgaccggttc gcgtagctca gtgaaattac
     5341 tgcaaccttt cctgacggaa atatggtaac actcgactcc acttatcatc ctgccaaggc
     5401 tggaatttta catgtgccgt ttctctggcg aggattgctc gcgctctgtt gagtatctgg
     5461 ggatattctt gctcgataga agtaaagcga ccggcttcgc ggtgctccgc gacctgaaga
     5521 agtggagtaa cgttctggca ggcagttaac atcacatcac ttgctcgcca taaccaagca
     5581 agtgtgcaaa gttcgttatc agtgaattgt tttgtgattg gggattgttg aatttctcga
     5641 tcgagaatat ccagcaccca gcggcggaac tctttggcta caggagttcg tgcaaacatg
     5701 gcgatcaggt gagcgccgcg cagggagaat acacgcactt tttttctgta atttcctgag
     5761 gtcactgatt cgatgacctg agtcataccg ctagtaaatt cgtcggcatt ctggttgaac
     5821 agattagtta ctgactttgc gcttttgtac tgtaaagcgt tcgcgatttc ggtagaggtc
     5881 agccagattc ctgtcatgcc agcaaccggt acaatcgctt taccttggaa gttcagatct
     5941 gatcttgcta caatattcat gtcgatattt cctacgcggt tattttcgat agaagcccct
     6001 gaggttgccg ccaacggggc ttcgctgttt ttagcgagca tgtatccgct ctctaaattt
     6061 caatgcccac acaagcgcct gaacaatagc ggcgttctca gacatccctt cctcctgtgc
     6121 aattcgcttg aattcatcct tcacattttg cggatagcga agggtgattt ttacttcttt
     6181 cttttccatt gatatctcct tgtgaggcca taatgacacc atgaggccat aatgccacca
     6241 ttgaattgat gtggcaatat ggcaccattc atttttttgg ggatttccaa tggctgataa
     6301 acaagtaaaa aactatgata agtttaacct ccgtttccct gatgggatgc gtgaagctat
     6361 agctgaacgt gccaagcgta atggccgctc aatgaactca gaaatcgtgc aaatacttga
     6421 agatgcgctt tatacagaca gtgcacgagt gttcaccgtt gaagatgaag tgaatcactc
     6481 agggaatgta gcaatagatc ccgtgcaact tgagaaatta atggaaaaag tgataaacaa
     6541 ggcattaatg ggctttacag gatcgcctgc aaaaaaaatt gataaaaaat aaaaaaatta
     6601 ttagaaactt ctgctatatg actaaggata aatgatgagt aaatttgatc gttatttaca
     6661 aagagaaatt ctggctttag ctattgatgc ctaccctgct tatattggtc ataaacactc
     6721 taaaattcca gccagctatg agttattaaa aaatgccaaa gaagatttgg tgtatcctaa
     6781 tatacattac ttgcatgaac acagcttaat aaatttcgat aagataggat atagaaataa
     6841 caacagtaat cccttagcat gggtaaaagc aacctctcac ggcattgatt tcgttcagca
     6901 ggatggaggc ctttctgcca ttctaaacgt caaaacaatc aggtttcata aagacactat
     6961 agttgtactg gaagacctca tagcgatttc aaatatgaat gatactgata aagaaaaggc
     7021 taaatctatt ctttctgaat taccagttga tgcaattaaa acagttgtac aaacacttac
     7081 aactacagct ctaaatgcgt tgctaaaatc ataaacgctc ctatgaaagg cttgtcctta
     7141 tggttatgac ttaggaggat catcgttatc cctcaatcaa taaatataaa tttaccacga
     7201 cgaaattaaa tatttttaat acaaccggca ttaacactta aagcaataat agtgagttat
     7261 taagatgtaa tacggtaagt tgaagtattt tctcctttat aacagttcaa aacattaaat
     7321 ttattgacca atttgaccat gccagaattt acgtttacca ctaccatttc aacaactggc
     7381 agtttcttaa gcaatgaaga aattacttga aatcctattg attttccctg catgctttta
     7441 gctatatgaa tttcatgcaa aaatatgtaa ccttcacgag ggattgatat atccaaattc
     7501 atatattcaa tctctacata cccaactaaa taaccttggt gctttataaa atagatattc
     7561 tgcacatcat tgatttcata aactttttgg ctccagccat aactagaacg gtaggtattt
     7621 aacgttataa gttttttatc atcaggtaga ttacaagaag caaacttaac aaaattaacc
     7681 ataatctaac cttccgttaa tttccgaata gttataataa aaacataatc gatttgccgt
     7741 ttcttatcca caatatggta gactcgtgaa cgtgctataa tgatattata tcataccaac
     7801 atttttatag ctctccctaa attttcgtct tcagtgggcc acctgaaaac accgtttgtc
     7861 atgtttttct acctagcgcc ctctttcgcc agaatacctt cctcccggtt gatactgact
     7921 atggtcgcac tagtcgattc tgcaacatca gtcgcacaga aattctggtg cgtttccaag
     7981 tagtgaataa ttgcctcttt tcccgtcata cactggctcc tttcagtccg aacttagctt
     8041 tgatttctgc aatcttcgcc agagcctgtg cacgatttag aggtctaccg cccatgacag
     8101 gaagttgttt tactggttca ggtatagcct caccacggtt aattctcgca gtcatatggg
     8161 caagttcatc ggcagccttg cgccgtaatt ccgcgtcagt caacgcattg gcccgcatgt
     8221 tctggtacag gttggtaacc aaccagtagt gcgcgtttga tttccatgga taagactctg
     8281 cgtccggata caggccacgc ttccggcaat actcgtaaac catatcaacc agctcgctgg
     8341 cgtttggcag cccggctgta acggatgttt cttcccggca ccaggcaaca aactgcccgg
     8401 gtgatggcag gaatggtcga ttctgccgac gggctacgcg cattcctgcg ttaacctgtt
     8461 ccattgtggt gatcccgttt tcccggaaag ccagcaccca ctggcggcgg atttcgttca
     8521 gttcgttctg gtcccggtta gccaggctcg ccgggaaagt tgccagtaac tggctgaaca
     8581 caccattgat gatctgcgct acctgctgta cctgcggctt ttcgtcgtac tgttccggca
     8641 tgttattggc gatccgacgc atctgctcac ggtcaaagtt aaccatctgt gcggcgatgt
     8701 ttttcataaa tccaccccat aaatccagtc agtgtttgtc aggtcgagtt ttgattttcc
     8761 ggctgtcacg ccagcctgtt gcttgttacg gttgatttcg agttgggtcc acttgtcgcg
     8821 gagtttggcc ggacatagca cgttaccgga ccagaagttg tcctggcatg cccagcggaa
     8881 cagcacgcac atgtcgcggt ggttacgtcc gtcacgttca cgcatcaggc ggatatcgtt
     8941 agcccaccct gcaaaattcg gttttctggc tgatggcgcg atggtcttca ccatgtcaaa
     9001 catccactct gcggcggtca ggtcttctgc tgtcccccac ttgctgccgc tctgaattgc
     9061 agcatccggt ttcaccacag gaagatcgtt ttctggctgg tcagaggatt cgtcagaatt
     9121 ctcggacgaa aaaggtttta tattgtcttt tgttagtttg tcttttgtgt ttacctgatt
     9181 cgggtaaacg cctttacctg atttgggtaa acttttctta cctgattcag gtaaatttac
     9241 ctctttcagg taaactttat ttttcttacc tgattcgggt aatgttgacc attcactgac
     9301 cacattatta atgccggtat tccgcccgct ctgaataaga atcccacgct ttaccagaac
     9361 actttttgca gcagaacact tgtgcggcaa tatcccggtc aattcggaca gttgctcgtt
     9421 gctcacccaa tccagttttt tattaaagcc atatgttttg cgcatgacag ctaggaagac
     9481 cagaagctgg tgctgtgtta atccggccag catcacagct tccagcaact catttgcaat
     9541 gcgcgtataa ccatcatcga gatctgccac gcgcggctcc ttttgtgccg catccggcac
     9601 tggaaaattg aatatctcag cagtgtttgc cataattcct cccgcaatga gtgtgttacg
     9661 atttgcacct gaaagtcggt tctgttccag cagaccggct ttcgccattt ctgaacctgt
     9721 catatcgccc ccagcatggt agtaaccatc gccatcaatg gaccagccag atctgggtcc
     9781 acacgaaaca tcgacacaat accttcacta atttccttca gtttctggtg gcgtggtgcg
     9841 ttgagaatga cagcctgttt tgcctcactg agttcctttt ccatttcagc caacctagcc
     9901 atgaagctat cctgctcaac caggtaaccg cgatattcca gcggtagtac cgccagaatt
     9961 gccggggtca gttcacgcac gttatttcgg tatttttcag aatcgaattt gttatcgagg
    10021 aagcggaaca gcttctggcg tgcacggctg acatcatcag ggaaatcgat ggtgccgccg
    10081 ccctgctccc gatactcatt cacaatgagt gtggcaacga catcctgatt atcttcagcc
    10141 gaccaagcgc ggacggcatc acggattttt tcgtggcctg gcacctgttt tgtttgagaa
    10201 cgatttatca ccgcagtcgg gctaaatccg ctagtctgtt ggtatgtaag tggttgcata
    10261 attgactcct ttagtttgaa ttgactgtta agttgattgc ttattgttaa agagcgtgaa
    10321 atggaaattt aagctgcgtt cttttcggtg tgtggaaaca acttcggaag atccgggcga
    10381 atctggtatg ccttcactac tccaccagta gccgtaacaa tgctgccgac atgttcaggg
    10441 gatacctttg ctttgttgtg aagccactta tagacggcct gctgtgaaac ttcgcaagca
    10501 gcgcccagtt tcttttgtga accaacgata ttgatcgctg ttttgatagc tgggttcata
    10561 acaacctccg tggttaattt gaatcaagat taaaactatg gttgttttta gtcaacaacc
    10621 attttcgttt gatggaataa aaccttggtt gtacatttgg actatgaaaa caacactctc
    10681 agaaagactt aaagaagcca gattagcgcg aggccttaca caaaaggcgc ttggggattt
    10741 ggtcggggtt agccaagctg ctattcagaa aatcgaaaca gggaaagcta atcaaacaac
    10801 taaaatcgtg gagatcgcga acgctttggg tgtgcgcgca gaatggttat cttctggcgt
    10861 tggaaatatg tcagacagta cagtgcaacc aatacaatca actgtcagcc attccaaata
    10921 cttcaagatt gacgttcttg atatagaagt cagtgctggg ccgggagtca tcaaccgtga
    10981 gtttgtagaa gttctacgct cggttgagta ctcgtttgac gatgctcgtc acatgttcga
    11041 tggtaggaag gcggaaaata tccgcatcat taacgtgcgt ggtgacagca tgtcaggaac
    11101 gatcgaacca ggtgatctgc tgttcgttga tatcacagtt aaatctttcg acggtgatgg
    11161 tatctatgcg tttctgtacg acgacacagc ccatgtaaag cgcctgcaaa tgatgaagga
    11221 taagctgctg gtcatctctg ataacaaaag ctactcaccg tgggacccga tcgagaaaga
    11281 cgagatgaac cgggtgttca tcttcggtaa ggttattggg agcatgccgc agacatatag
    11341 gaagcatggt taaagtgagg ctaaaaaaca gttacagcaa taggcctgtt gtttttcttt
    11401 aaacacgcag tgttaaaccg ctctttgaga tgcggagtaa tgagatggaa gacttgaatc
    11461 acataagggt
//
LOCUS       DAEVZA010000148        11438 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEVZA010000148 DAEVZA010000000
VERSION     DAEVZA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.148"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            137..1315
                     /locus_tag="LAF92_004210"
     CDS             137..1315
                     /locus_tag="LAF92_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HBI9793131.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQANNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPMKNIMDHTK
                     "
     gene            1312..1806
                     /locus_tag="LAF92_004211"
     CDS             1312..1806
                     /locus_tag="LAF92_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793132.1"
                     /translation="MMTKTQINKLIKMMNDLDYPFEAPLKESFIESIIQIEFNSNSTN
                     CLEKLCNEVSILFKNQPDYLTFLRAMDGFEVNGLRLFSLSIPEPSVKNLFAVNEFYRN
                     NDDFINPDLQERLVIGDDSISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIM
                     DSCS"
     gene            1901..2056
                     /locus_tag="LAF92_004212"
     CDS             1901..2056
                     /locus_tag="LAF92_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793133.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            2256..2540
                     /locus_tag="LAF92_004213"
     CDS             2256..2540
                     /locus_tag="LAF92_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HBI9793134.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(2578..4320)
                     /gene="ggt"
                     /locus_tag="LAF92_004214"
     CDS             complement(2578..4320)
                     /gene="ggt"
                     /locus_tag="LAF92_004214"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBI9793135.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            4440..4880
                     /locus_tag="LAF92_004215"
     CDS             4440..4880
                     /locus_tag="LAF92_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBI9793136.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(4867..5610)
                     /gene="ugpQ"
                     /locus_tag="LAF92_004216"
     CDS             complement(4867..5610)
                     /gene="ugpQ"
                     /locus_tag="LAF92_004216"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBI9793137.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(5607..6677)
                     /gene="ugpC"
                     /locus_tag="LAF92_004217"
     CDS             complement(5607..6677)
                     /gene="ugpC"
                     /locus_tag="LAF92_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HBI9793138.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(6679..7524)
                     /gene="ugpE"
                     /locus_tag="LAF92_004218"
     CDS             complement(6679..7524)
                     /gene="ugpE"
                     /locus_tag="LAF92_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBI9793139.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(7521..8408)
                     /gene="ugpA"
                     /locus_tag="LAF92_004219"
     CDS             complement(7521..8408)
                     /gene="ugpA"
                     /locus_tag="LAF92_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBI9793140.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(8506..9822)
                     /gene="ugpB"
                     /locus_tag="LAF92_004220"
     CDS             complement(8506..9822)
                     /gene="ugpB"
                     /locus_tag="LAF92_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBI9793141.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(10218..10931)
                     /gene="livF"
                     /locus_tag="LAF92_004221"
     CDS             complement(10218..10931)
                     /gene="livF"
                     /locus_tag="LAF92_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBI9793142.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(10933..>11438)
                     /gene="livG"
                     /locus_tag="LAF92_004222"
     CDS             complement(10933..>11438)
                     /gene="livG"
                     /locus_tag="LAF92_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBI9793143.1"
                     /translation="VRLFREMTVIENLLVAQHQQLKTGLFSGLLKTPSFRRAQSEALD
                     RAATWLERIGLLEHANRQASNLAYGDQRRLEIARCMVTQPEILMLDEPAAGLNPKETK
                     ELDELIAELRNHHNTTILLIEHDMKLVMGISDRIYVVNQGTPLANGTPEQIRNNPDVI
                     RAYLGEA"
BASE COUNT         2944 a         2987 c         2727 g         2780 t
ORIGIN      
        1 tcataattac ttcctaaagt gtaatatttt attttttaat atatacgcct acaatttcct
       61 ggagtaaata aataacaatt aacaagcata atattgccat tgataaaata gcatgccata
      121 aaaggacttt tcagggatga gtaatattgt ttacctgaca gtaacgggag aacaacaagg
      181 aagcatctcc gcaggttgtg ggacttctga gtctacaggt aatcgttggc agagcgggca
      241 tgaggatgaa atatttacat tctcactctt aaataatatt aataatacgg ggcttggttc
      301 acagttccat ggtataacat tttgtaaatt aattgataaa agcactccat tatttattaa
      361 ttccattaac aataatgaac aattatttat gggatttgac ttctatcgaa taaatagatt
      421 tggtagattg gaaaagtatt attatataca actaagaggc gcttttttat cggctattca
      481 tcaccagatc attgaaaacc aactggatac agaaacaata actattagtt atgaatttat
      541 cctctgtcaa catcttatcg caaataccga gttcagctat ttggcactcc ctgaaaatta
      601 taaccgtttg tttttaccaa attcaaaaaa ccaagcaaat aatcgtttca aaacgttaaa
      661 cagcaaagct attggcaggc tacttgctgc tggtggcgta tacaatggga acattgaagg
      721 attcagagat actgcggaaa aactgggtgg agatgcaata aaaggctatg atcaaatact
      781 aaatgaaaaa acagcgggca tagcgatagc aacagcatct attcttttaa caaagcgttc
      841 taatgttgat acatatacag aaataaatag ttacttaggc aaacttagag gtcaacaaaa
      901 acttcttgat ggtatagaca taatagaaat aatatacatt aagagacctt caaaagactt
      961 agctaactta cgaaaggagt ttaataaaac tgtaagaaaa aattttctta tcaaacttgc
     1021 aaaaacctcc gaagcatctg gaagattcaa cgccgaagac cttttaagaa tgagaaaggg
     1081 caatgttcct ctaaattata atgttcacca taaactatct ctagatgatg gtggtactaa
     1141 tgatttcgaa aatttagtat taatcgaaaa cgaaccatat cataaagttt ttactaacat
     1201 gcaatcacga atagctaagg gaatattagt aggtgaaagc aaaatcactc cctgggccat
     1261 tccatctggc tcaatttatc ctcccatgaa aaatattatg gaccacacaa aatgatgact
     1321 aaaacccaaa taaataaatt aataaaaatg atgaatgatt tagactatcc atttgaagca
     1381 ccgctcaagg aatcatttat tgaaagtata atccaaatag aatttaattc taattcaact
     1441 aattgcctgg agaagttatg taatgaagtt agtattcttt ttaagaatca acctgattat
     1501 cttacttttt taagagcaat ggatggattc gaagttaatg gattacgatt atttagcctc
     1561 tcgattccag aaccttcagt taaaaacctt tttgccgtaa atgaatttta tagaaataat
     1621 gatgatttca taaaccctga tctacaagaa cggttagtga tcggggatga tagcatttca
     1681 atatttactt atgacattaa aagtaacttt tttgaaatta gagataatat tggcacagaa
     1741 aatatattca gttcgtttag tgatttctct tcattcttaa acgaaatcat ggacagttgt
     1801 tcataataat caaaaggaaa caaaaatcat tgtaaaccat gccatctttt tagagattac
     1861 cagcagaaaa ttccttgttc gaaatttagc agtgcaagct atggaaaaac ttacaacgga
     1921 actacactcc ttaagtgaga tggaccgaag acatgtagtc tctattttag ccgaaatagc
     1981 taatggctat gatgatttta atgacatgtt gatttattta gaattttacc cgaatcataa
     2041 gattctgata tcttaaataa gaaaatcccg ttcgacgttc tgaaaaagaa atcataaaat
     2101 attagcagat acttatttcg tgactatatc ttatccgatg aacttttttc aaaaccagca
     2161 tttaataata tctttgacga agatgaacgc gtctttgctc aatgtatcac acaggaataa
     2221 taatataaca ctcaacaata atatttatta taattatgat tacttatcac gacgcattcg
     2281 cgaaagcgaa ccattacctt gatgatgcag atctcccggt cgtcattact ctacatggac
     2341 gctttagcca gggctggtat ttctgtttcg aagcacgaga atttctcgaa actggagatg
     2401 aggccgcgcg cttagctggt aacgcacctt ttattattga taaagacagt ggtgaaattc
     2461 attctctggg aacggcaaag ccgctggaag aatatctaca ggattacgaa ataaaaaagg
     2521 ctaccttcgg cttgccctga caaaatagcc ctcttcccac gaagagggcc gctaacctta
     2581 gtaccccgcc gttaaatcat ccaccgagcg cgggtcggat gcgccgtaca actcaccgtc
     2641 cggcccaacc ataatgcttt gtgtactgcc catcgcctct ttcagcgcca ctttctgacc
     2701 ttttgcttcc agcagcttga gcgtatccgg gctaaaccct ttttcgacac gcagctcgtc
     2761 cggcaaccac tgatggtgga aacgcggcgc attggtcgct tcggcgacgt tcatgccata
     2821 atcgatgcta ttcaccacca tttgcagcac tgtagtgatg atccggctac cgcctgggct
     2881 accggtaacc agccaggttt taccgtcttt caccacaatg gtcggcgaca tcgacgacag
     2941 cgggcgtttg ttcggcccga cggcgttggc atcaccgccc accagcccgt aaacgttcgg
     3001 tacgcccggt ttggcggaga aatcatccat ctggttatta agcagaatac cgctctcgcc
     3061 cgcgacaata cccgtaccga aggtggtgtt cagcgtatag gtcaccgcca ccgcgttacc
     3121 gtctttatcc actacagagt aatgggtagt ttgattactc tcataaggcg caagcttgcc
     3181 agggcgaatc tcactggacg gcttcgcttt gttgatatca atttgatcgg caatggattt
     3241 ggcataggct ttattggtca gtgcctgcca cgggactttg acaaaatccg ggtcgccaag
     3301 atattccgag cggtcggcgt aggcgtattt ctccgcttct gccatgattt gcatcgcatc
     3361 ggcgctgcca aagccgtatt tcttcatatc gaagttttcc agaatattga ggatttgtac
     3421 gatatggatc ccgccggagg atggcggtgg catggagtaa acctgatacc cgcgataatc
     3481 gccgcttatc ggagtgcgtt cgaccgcttt ataggccgct aaatcttctt tagtgatcaa
     3541 gccaccgttt ttctgcatct cctgggcgat ctgttccgca atcgtgcctt tatagaattc
     3601 gtccgggccg ttttcagcaa tcatctccag gctctttgcc aggttcgcct gcaccagcgt
     3661 gtcgcccttt ttcagcggct cgccctcttt ccagaagata gctttactgt tttcgtgatt
     3721 cggcaacact tcgctaccgt aggttttgag atcgtcagcc agcgcgtcgt taacgataaa
     3781 accatcgcgt gccagtttaa acgcgggctg cacgactttg ttcagcggca tggtgccgta
     3841 tttatccagc gccagcgaga aacctgctac cgtacccggt gtgccggaag ccagatgcga
     3901 agtgagtgat tttttgctgt ccgggttgcc ctgatcatcg aggaacatat cgcgggtcgc
     3961 tttggcgggt gccatttcgc ggaaatcgat agcggtggta ttgccatttt tcgagcggat
     4021 taacataaaa ccaccaccgc ccagattccc tgcctgcgga tgcgttaccg ccagcgcgta
     4081 gcccaccgcc acggcggcat caacggcatt cccgccctcc ttgagaatat ccacccccac
     4141 ctgagtggca gtggcgtcca cagacgctac cattccctgt ttcgcgcgta ccgggtggaa
     4201 gacatcttcc tccacaccat acgagacggg cggcgcagga ggcgcggcgg cggcgctaaa
     4261 acaacttcct gagagcagag cagcaatggc cacccggcgt aaaaacgtcg gttttatcat
     4321 cgttattctc cagagattaa ggggcaaccc cagctaagcc tggtatataa ctctgaatta
     4381 atcatcgttt tgtcgggaag cgagtaaact taaaggatat cttcagagga ggatacgcga
     4441 tgaaacgact tctgattctt acggcactcc tgccgtttgt cggctttgca cagcccatta
     4501 atactctgaa caaccctaac cagccggggt atcagatacc cagccagcag cggatgcaaa
     4561 cccagatgca gactcagcaa atccagcaaa aagggatgct gaatcagcaa ctgaaaacgc
     4621 aaactcagtt gcaacagcag catttagaaa accagataaa caataattct cagcgggtgt
     4681 tgcagtcaca gccgggggag cgaaatcccg cccggcagca aatgctgccc aacaccaacg
     4741 gcgggatgtt aaacagcaac cgtaatccgg atagttcgtt gaatcagcag catatgctgc
     4801 cggagaggag aaacggcgac atgctgaatc agcccagcac gccgcagcct gacattccgt
     4861 tgaaaactat tgggccgtaa agttcggacc aatcacgtca atcgcatcgg tacagatgca
     4921 atccacgccc cagcgcagca actctgccgc gcgctggggt ttattgacgg tataaaccag
     4981 aatccgcagt ccggcgtctt tcaactgcat cactcgcact ttatcgagta acttatgatt
     5041 gagatgaata gagacgcagc ccagccgcgc ggtcagttcg cgccagtcgt cgcgccactc
     5101 atcaagcaat aaaccacgcg gcagttccgg tgccgccagc tgtgcagctt ctaaagcatc
     5161 aatctcaaac gatgacagca gcggcggcgt cataccggcc cacagttggc gtgccgccag
     5221 cgccaccatt ttgcccgtta atggtccagt gccagtggtg ggtttgattt cgatattcgc
     5281 catcatcccg tgttcgcgac agcgttccgc cacctgcgaa agcaacggca gcggctcacc
     5341 tttaaacgct ttgctgtacc aactgcccgc atccacgcgc agtaaatcct gccagttcag
     5401 ttcacccgcg acgccccagc cgttgctggt gcgttcgaga ttgtcgtcat ggagcaggaa
     5461 gatctcgcca tctttcgata acttcgcgtc aaattcgatc atcttatgac cgtattttgc
     5521 cccgacgtcg attgccgcca gggtgttttc cggggccagc ttaccgccgc cacgatgagc
     5581 gacgatgcgg ggataaggcc agttactcat actcgttgtc ctgtttcacc atcaaaaaga
     5641 tgcagctgat tttccgccag atgcagccac agcgtgctgc ctgccgtcgg gcgctcctga
     5701 tgcgccagtc gcaccaccag cttctgctcg ccccagcgtc cgtgcgccag gttatctgcg
     5761 ccgaggatct ccagcgtgtc catcaccagc ggtacgccgc cttctgcctg cgagcttagc
     5821 gcaatatgtt ccgggcgaat gccgagagtc attttacgcc cggcatactg acggtagcca
     5881 ccgtttagcg gcagctcaat accgccgtcc agttcgaaat gcgtgccttc gttattcacg
     5941 cggcctgtca gcaggttcat cgccgggctg ccgataaaac tcgctacaaa caggctggcg
     6001 ggcttttcgt agacttcaac tggtgtgcca atctgttcgg caacgccgcc gttcatcacc
     6061 attactcgct gggcgagcgt catcgcttca acctgatcgt gagtaacgta gagtgaagtc
     6121 gttttcaggc gacggtgcag ctgttgcagc tcaaggcgca tctgcacgcg cagcttggca
     6181 tcgaggttag agagcggctc atcaaacagg aacaccgccg gatcgcgcac aatcgcgcga
     6241 cccatcgcca cacgctgacg ctgaccgccg gatagctcgc gcgggcggcg cttaagcagg
     6301 ccatccagct ccagaatgcg cgccgcttct ttaacgcgct cggctatttg ctgcttgccc
     6361 atgccgcgaa ttttcagccc ccacgccatg ttttcttcga cgctcatatg cggataaagc
     6421 gcgtagttct ggaacaccat cgcaatcccg cgatctttcg gctccatttc ggtcacgcgc
     6481 tggtcgttga tccagatatc gccttccgtc acccgctcca gcccggcaac catgcgcagc
     6541 agcgtcgatt ttccgcagcc agacggcccg accatcacga taaattcgcc atccgccaca
     6601 tcaagggtca gtggtttaat cacctgggtt ttgccatccc agcttttggt tactgcctgt
     6661 aatttcagtc ctgccatctt atttctcact atcgaccagg ccgcgcacga aggcacgctg
     6721 catcactaaa acaatcacca ccggagggat aagcgttaac aacatcgccg ccattaccga
     6781 gttccattcc gtggtgcctt cgccagtggc gatcatccct ttgatccctg ccacggtggt
     6841 gccgagatca acgtcggtaa taatcaacaa cggccacaaa tactgattcc agccgtagat
     6901 aaaggtgatc acaaacagcg ccgccagatt agttttggag agcggaaaaa cgatgtcgca
     6961 aaagaagcgc attggcgatg cgccgtcgat ccgcgcggct tccaccagct catccggcag
     7021 cgtcataaag aactggcgga acaggaaagt agcggtcgcc gaggccatca gcggcagcgt
     7081 taaaccggcg tagctgtcga gcattttcag gttggcgatg acttccaccg tcgggaagat
     7141 acgtacttca accggcagca tcagggtgat aaaaatcatc cagaagaaga ggttacgtag
     7201 cggaaaacga aaccagacaa tggcaaatgc cgagagcatc gagacggtaa ttttgccgag
     7261 cgtaatgctg aacgccatca caaagctgtt aagcaacatc cgccagaacg gcgcgctatt
     7321 cgtgcctacc ccgttcaccc agatgttgtg gatgttttcc agcagatgtg tgccggggat
     7381 gagcgtcatc ggcgcggcat agacggcctg tttatccagc gtcgccgcga caaacgccac
     7441 gtacagcggg aagaggatca ccgcgatccc gaggatcagc atggtatggc tgaatatcgt
     7501 cagccacgga cggttctcaa tcattggtaa cgcaccttgc tttcaacata gcggaactgc
     7561 accaccgtca gcacgatgac gaggaacatc aacaccaccg actgtgcggc agacgaagcc
     7621 agatccagtc cggtaaaacc ttcgcggtag atcttataaa tcagcgtcgt ggtggcctga
     7681 accggcccgc cggacgtggc ggcgtcgatc accgggaagg tgtcgaagaa ggcatacacc
     7741 aggttcacta ccagcaggaa gaaactcacc ggggcgataa gcggcagcgc aatcttaaag
     7801 aagcggcgaa tcggccctgc accgtcgatg gctgcggctt cgatcaacga acggggaatg
     7861 gattgcagcg cggcatagaa gaacaggaag ttgtagctga tttgcttcca tactgaggca
     7921 aacaccacca gaaacattgc ctgaccgctg ttttgcgcgt ggttccagtc gtagccgaac
     7981 tccgcgagaa aatgggtgat cagcccgcga ccggggttaa acaggaagat ccacaatacg
     8041 gcggcaacgg cgggagccac ggcgtaaggc agcaacatta aggtttgata gaaacggctg
     8101 ccgcgcacga tgtactccac cagcgccgcg aagaacagcg acaccagcaa accgctgacg
     8161 gtgacaaagg tgctgaattt tatcgtcgtc cagaaggagt cgagatagta gctgtcatga
     8221 aacagcgcga cgaagttatc caggccgaca aactggctgg agaaaccaaa cggatcgacg
     8281 ctttgtagcg agtaccacaa cgcttcgccc gcaggccaga taaaaaagat aacggtgatg
     8341 atgagctgcg gcgcgaccag cagataaggc agccagcgcg agcggaacac cggacgggat
     8401 gatgacatta cggttaattc ctgaactgtg ccggatgcgc ttcgcttatc cggcctacac
     8461 gcgatgccag gtcagataag cgaaacgcat ccgacattac actgattaag acttcgtcga
     8521 tttctcaaag cggcgcagca actgatttcc acgctcaacg gcggtatcca gtgcctgctg
     8581 tggtgtcttc ttaccggtcc acacgctctc cagctcttca tccacaatca cgcggatctg
     8641 cggcatgttg cccagacgca gccctttggt gaacggcaac ggcggcttat tcagcatctg
     8701 acgcgtcgcg gtatccgccc ctgggttttt ctcataaaag ccctgctcac gggtcaggtc
     8761 atacgctgct ttggtgattg gcagataacc ggttttctga tgccactcgg cagcgttttc
     8821 tggcttcgcg aggaagtcga ggaacttcgc cacgccggta tacgtttctt tatctttccc
     8881 ctgcatcacc cacaggctgg ctccgccgat aatggcgttt tgtggcgcat ctttcgcatc
     8941 ggcgtcgtaa ggcatcatac ctacgccgta gttaaatttg gcgtactcgc gaatgttggc
     9001 aagagaaccg gaagaggcgg tggtcatcgc gcaatcaccg ttatagaact tctcggtgga
     9061 ttcatcctta cgcccgacgt agctgaaatc gcccttcttg ttcatctcct cgagcatggc
     9121 gatgtgtttc acctgctccg gcttgttgaa ctccagcacc gcgtccgtgc cgtcaaagcc
     9181 gttgtttttg ctggcaaacg gcagaccgtt ccaggcgcta aagttttcca gttggatcca
     9241 gccctgccag ccgctggcgt agccgcactt cataccggag gctttcagtt tcgcggcata
     9301 gtccgccaga tcctgccagg ttttcggcgg ttgttccggg tctaatcctg ctttcttgaa
     9361 ggcgtctttg ttgtaataga gaacgggggt cgagctgttg aatggctggg agagtaagtg
     9421 gcccgttttg ctgtcggagt agtaaccgga aaccgtcggc acaaactgcg actcatcgaa
     9481 ttgaatcccc gcctctttaa acacgtcata taccggttta atggctttcg atgccatcat
     9541 ggtggcggtg ccaacttcat aaacctgcaa aatagccgga gcgttgccgg tacgaaatgc
     9601 ggcaatcccc gcgcttaaat tctgttcgta gttgccttta taggtcggta caattttgta
     9661 atccggattt tcggcgttaa aacgttgggc cagagaatcc acctctttac ccagttcccc
     9721 ttccatagaa tgccagaacg gaatggtcgt cactgcctgt gcattcccca ttaacgccag
     9781 tccgagcgcc agtgctgaag ctgtataatg taacggtttc atcgtttatc tctcttgttg
     9841 taccgaatgc gcgaattcac gcgttttatg ctcgcggggt aacatgacat gctcgaatta
     9901 cagaaaaata acttttttgt tacatttgta agatagtaag gtgtcagaaa gatgacaagg
     9961 cggtgacggc gtgggtgagg gaaaatggga gatggggcac ggataagcgg aaaatagaag
    10021 gtctgaatca aactctacag attactcatc gtttcatgcc agatgcggcg taaacgcctt
    10081 atccggccta caagatcgtg caaattcaat atattgcaac tcacccagta ggcctgataa
    10141 gcgcagcgca tcaggcaatt ttacatttgt cacctgtctc aaaggagtct tttgactcct
    10201 tatcaatcaa cgtgttatta cccgcctaaa tacgcacttc tcaccgcttc gttcgccagt
    10261 agtgcatcac cagtatcgga gagcaccaca tgaccgtttt ccagcacgta gccgcgatca
    10321 gcaagcttta gcgcctggtt ggcgttctgc tcgacgagga agatagtcat cccctgctcg
    10381 cgcagctgct cgatggtgtc gaaaatttgc tggatgataa tcggcgcaag accgagcgat
    10441 ggctcatcaa gcaacaacaa acgcgggttg ctcatcagcg cgcgaccaat cgccagcatc
    10501 tgctgttcac cgccggacat ggtgcccgcc cgctgaacac ggcgctcatg cagacgtgga
    10561 aacagttcat agacccactt tatgcgctcc tgaaactgat cacgttcggc aaaaaagccg
    10621 cccatcgcca ggttctcttc caccgtcatc cgcgagaaga cgcgacgccc ttccgggaca
    10681 atcgctaccg cttcgcgcat gattttcgcc gtctgccagt cggtaatgtc tttatcatca
    10741 aacacaattc gcccactggt ggcgcgcggg tcgccgcata atgtgccgag caaggtggtt
    10801 ttccccgccc cgttcgcgcc aataagggtg acaatctcgc cctgattgat atgcaggctc
    10861 acctcatgca gcgcctggat tttgccgtag tgggcgctga ctttgtcaaa ggacaacatg
    10921 actttttcca tcttatgcct cacctaaata ggcacggatc acgtccgggt tattacggat
    10981 ttgctccggc gtaccgtttg ccagcggcgt cccctgattg accacgtaaa ttcggtccga
    11041 aattcccatc accagcttca tatcgtgttc aatcaacaag atagtggtgt tgtgatgatt
    11101 gcgcagttcg gcaatcagct catccagctc tttggtttct tttgggttaa gacctgccgc
    11161 cggttcgtcg agcattaaaa tctccggctg cgtcaccatg cagcgggcaa tctccagacg
    11221 gcgttgatca ccataggcca ggttactcgc ctgacggttg gcgtgttcca gcaaaccaat
    11281 gcgctcaagc caggtggcgg cgcggtcgag cgcttcgctc tgggcgcgac ggaaagaagg
    11341 cgttttcaac aggccagaga acagcccggt tttcagttgt tgatgctgcg ccaccagcag
    11401 gttttcaatc accgtcattt cacggaacag acgcacat
//
LOCUS       DAEVZA010000149        10952 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEVZA010000149 DAEVZA010000000
VERSION     DAEVZA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.149"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..202)
                     /locus_tag="LAF92_004223"
     CDS             complement(<1..202)
                     /locus_tag="LAF92_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793144.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALP"
     gene            complement(362..574)
                     /gene="rpmE"
                     /locus_tag="LAF92_004224"
     CDS             complement(362..574)
                     /gene="rpmE"
                     /locus_tag="LAF92_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBI9793145.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            777..2975
                     /gene="priA"
                     /locus_tag="LAF92_004225"
     CDS             777..2975
                     /gene="priA"
                     /locus_tag="LAF92_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBI9793146.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRIRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3131..4156
                     /gene="cytR"
                     /locus_tag="LAF92_004226"
     CDS             3131..4156
                     /gene="cytR"
                     /locus_tag="LAF92_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBI9793147.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4248..5207
                     /gene="ftsN"
                     /locus_tag="LAF92_004227"
     CDS             4248..5207
                     /gene="ftsN"
                     /locus_tag="LAF92_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBI9793148.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5300..5830
                     /gene="hslV"
                     /locus_tag="LAF92_004228"
     CDS             5300..5830
                     /gene="hslV"
                     /locus_tag="LAF92_004228"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBI9793149.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5840..7171
                     /gene="hslU"
                     /locus_tag="LAF92_004229"
     CDS             5840..7171
                     /gene="hslU"
                     /locus_tag="LAF92_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBI9793150.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7238..8164
                     /gene="menA"
                     /locus_tag="LAF92_004230"
     CDS             7238..8164
                     /gene="menA"
                     /locus_tag="LAF92_004230"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBI9793151.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8257..8742
                     /gene="rraA"
                     /locus_tag="LAF92_004231"
     CDS             8257..8742
                     /gene="rraA"
                     /locus_tag="LAF92_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBI9793152.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8827..9072)
                     /gene="zapB"
                     /locus_tag="LAF92_004232"
     CDS             complement(8827..9072)
                     /gene="zapB"
                     /locus_tag="LAF92_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBI9793153.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9497..10342
                     /gene="glpF"
                     /locus_tag="LAF92_004233"
     CDS             9497..10342
                     /gene="glpF"
                     /locus_tag="LAF92_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBI9793154.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10365..>10952
                     /locus_tag="LAF92_004234"
     CDS             10365..>10952
                     /locus_tag="LAF92_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HBI9793155.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIH"
BASE COUNT         2606 a         2835 c         2943 g         2568 t
ORIGIN      
        1 cgggcagggc gatgtcggtt tcaccgggaa ggacctttgc accgagcagg ggattgaccg
       61 gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg ggggcaccga
      121 tgcgcacccc ggctgaaccc tggacaatgc taccgccata ctgcgtcatg tcgccctgac
      181 gcgccgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa ccagaagcct
      241 agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt tcagaattta
      301 taaaaggcaa attacaggca caaaaaaagc gccgtgcggc gcttttttcg gaaatccggt
      361 cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg ccaccggtag
      421 caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg tcgaggttca
      481 ggtcatgacc aacggtggag cggattttca ttacgttacc gcaagagcag ctagcagtaa
      541 tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt aaggccgcgt
      601 cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct tcaatacgat
      661 catttcgtac gaaggcgcga aatcatacag aaattaacca gcgtatgcaa actgatccgc
      721 actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat gatgctatgc
      781 ccgttgccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat ctgctgccag
      841 aaggcatgac ggttaaagct gggtgtcgcg tgcgcgtgcc gtttggcaaa cagcaggagc
      901 gcatcggggt tgtggtgtca gttagcgatg tcagcgaact gccgctcaac gagctgaaag
      961 cggtagttga agtgctggac gttgagccgg tgtttactca ctccgtctgg cgattactgc
     1021 tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat gccttgccga
     1081 ttttactacg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg tttgccaccg
     1141 aacaaggcca ggcggtggat ctgaacagcc tgaaacgctc acccaagcaa caacaggcgc
     1201 tggcagcgtt acggcaaggc aaaatctggc gtgatcaggt cgcggaactt gaatttaatg
     1261 atgccgcgtt gcaggcgctg cgcaaaaaag gtctgtgtga tttagcaagt gaaacaccag
     1321 agtttagcga ctggcgaacg aactatgccg tttctggtga gcggttgcga ttgaataccg
     1381 aacaggccac cgccgttggc gcaattcata gcgcggcaga tactttttct gcctggctgc
     1441 tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg gaaaacgtgc
     1501 tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca ccgcaaacta
     1561 tcgcccgttt tcgtgaacgt tttaatgccc ccgtggaagt tctgcattcc ggcctgaacg
     1621 acagcgagcg gctttcggcg tggttgaaag cgaaaaatgg cgaggcggcg attgtgatcg
     1681 gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc attgatgaag
     1741 agcacgacag ctcctacaag cagcaggaag gctggcgcta tcatgcccgc gacctggcgg
     1801 tgtatcgtgc gcatagcgag caaatcccga ttattcttgg ctccgcaacg cccgcgctgg
     1861 aaacgttatg caacgtccag cagaaaaaat accgcctgct gcgcctgacc cgtcgggcag
     1921 ggaatgcgcg tccggcaatt caacatgtgc tggatttaaa aggtcagaag gtgcaggcag
     1981 gtctggctcc ggcgttaatc acccgtatac gccagcattt acaggccaac aatcaggtta
     2041 tcctctttct taatcgtcgg ggttttgcgc ctgcgctgct gtgccacgac tgcggctgga
     2101 tagccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa cagcatttgc
     2161 gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc tgcggttcca
     2221 cgcacctggt ccccgtgggg ctgggcaccg aacagcttga acagacgctt gcgccgatgt
     2281 tccccggcgt gcccatttca cgtatcgacc gcgataccac cagccgcaaa ggggcgctgg
     2341 aacagcaact ggcggaagtg catcgcggcg gcgcgaggat cttgattggc acgcaaatgc
     2401 tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat gtggacggcg
     2461 cgctgttttc tgccgatttt cgctcggcag aacgtttcgc ccagctttac acccaggtcg
     2521 ccggtcgtgc cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg caccatccgg
     2581 aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc gaacaggcgc
     2641 tagctgagcg acgcatgatg cagctgccgc cgtggaccag ccatgtgatt gtgcgtgcgg
     2701 aagatcataa caatcagcat gcgccgctgt tcctgcaaca actgcgtaat ctgatccttt
     2761 ccagcccgct ggcagacgac aaactgtggg ttctcggccc ggttccggct ctggcaccta
     2821 agcgtggtgg ccgctggcgc tggcagatct tgttgcagca cccttcccgc gtgcgcttgc
     2881 aacacatcat tagtggtacg ctggcgctta ttaatacaat accggattcc cgcaaggtga
     2941 aatgggtgtt ggatgtcgat cccatcgaag gctaatgcgc aaacgttgcg aggcggatcg
     3001 aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc acgcgctatc
     3061 tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac gtgccaggcg
     3121 aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag acgttgccct
     3181 caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg ataaagtctc
     3241 ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt taccgcaacc
     3301 tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg tcccggatat
     3361 ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg caaatcacgg
     3421 atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa cctttatcga
     3481 tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggctcaaggc tgccgtttga
     3541 tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg aatttgcacc
     3601 ggaactggag ctgcctaccg ttcatatcga caatctgacc gccgcatttg atgcagtaaa
     3661 ttatttatat gagcaagggc ataaacggat tggctgtata gccggtcccg aagagatgcc
     3721 actgtgtcac tatcgcctgc aaggctatgt tcaggcgctg cgtcgctgcg gcattatggt
     3781 tgatccgcaa tacatcgccc gtggcgactt caccttcgaa gccggaagca aagcgatgca
     3841 gcagctgctt gatcttccac aaccgcctac tgctgtcttc tgccatagcg atgtgatggc
     3901 gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag acctttccat
     3961 aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga caaccatcgc
     4021 gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc aaatgcaggg
     4081 gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc ggggatcaac
     4141 acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag gcccgccgct
     4201 taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg gcacaacgag
     4261 attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc tcacggaaaa
     4321 agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc gccgttcttg
     4381 tgacctttat cggtggtctg tacttcatta cgcatcacaa gaaagaagag tccgagacgc
     4441 tgcaaagcca gaaagtgacc ggtaacggac taccaccaaa accagaagaa cgctggcgct
     4501 acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa ccttctgccg
     4561 gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt cttgaacaaa
     4621 tgcaggctga tatgcgccag cagccaacgc agctggttga agttccgtgg aatgaacaga
     4681 cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa ttagcggagc
     4741 agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag cagacgcgta
     4801 cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccggct tctactcagc
     4861 agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg aagccgcagc
     4921 aagctgcgcc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag aaaaaagacg
     4981 aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca gagacggtac
     5041 gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat ggctggaatc
     5101 gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc aatcggttga
     5161 agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac cctcaaaatc
     5221 ccccccatct ataattgcat tatgccccgt acttttgtac ggggtttgta ctctgtattc
     5281 gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg gccatgtggt
     5341 catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca acgtgaaaaa
     5401 ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta ctgcggatgc
     5461 ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc atctggtcaa
     5521 agccgccgtt gagctggcga aagactggcg taccgatcgc atgctgcgca aacttgaagc
     5581 actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg gtgacgtggt
     5641 gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc aggctgcggc
     5701 gcgtgcactg ttagaaaata ctgaactgag tgctcgtgaa attgctgaaa aggcgttgga
     5761 tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag aattaagcta
     5821 caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca gcgaactgga
     5881 taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc tgcgtaaccg
     5941 ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga aaaatatcct
     6001 gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg ctaagctggc
     6061 gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtgggct acgtcggtaa
     6121 ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg tacgcgtcca
     6181 ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta ttctcgacgt
     6241 gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg aaccgtccgc
     6301 tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca aagaaatcga
     6361 gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag gcatggaaga
     6421 gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc aaaaagcgcg
     6481 taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg cgaaactggt
     6541 gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg ggatcgtgtt
     6601 tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg atgtttctcg
     6661 tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt ccaccaaaca
     6721 cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc agattgcgaa
     6781 accgtctgac ctgatcccgg aactgcaagg tcgtctgcca atccgcgttg aactgcaggc
     6841 actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta tcaccgtgca
     6901 gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact ccggtattaa
     6961 gcgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg gtgctcgtcg
     7021 tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca gcgatttaag
     7081 cggtcaaact atcatcattg acgcagatta tgtgagcaaa catctggatg cgttggtggc
     7141 agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca tcattgtttg
     7201 atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac aaattagccg
     7261 aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct ttgctgcaat
     7321 tatcgtcggg acagcgctgg catggtggca aggtcacttc gatccgctgg tcgccctgct
     7381 ggcactgatt accgccgggc tattacagat cctttctaac ctcgccaatg attacggcga
     7441 tgcggtaaaa ggcagcgata aacctgaccg cattgggccg ctacgcggca tgcaaaaagg
     7501 ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc tcatctgtct
     7561 ctccgggctg gcactggttg cagtggcatg ccatacgctg gccgattttg tcggtttcct
     7621 gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca atcgtcctta
     7681 tggttatatc ggtctgggtg atatttccgt actggttttc tttggctggt tgagtgtcat
     7741 ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc cggcgaccgc
     7801 atgcggcctg ctggcaacgg cagtactgaa tattaataac ctgcgtgata tcaatagcga
     7861 ccgcgaaaat ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg cgcgtcgtta
     7921 tcatgcctgc ctgctgatgg gctcactggt gtgtctggcg ctgtttaatc tcttttcgct
     7981 gcatagcctg tggggctggc tgttcctgct ggcggcacca ttactggtga agcaagcccg
     8041 ttatgtgatg cgggaaatgg acccggtggc gatgcgacca atgctggaac gtactgtcaa
     8101 gggagcgtta ctgactaacc tgctgtttat tttagggata ttcctaagcc agtgggcagc
     8161 ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca tagcgcgata
     8221 tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac ttccgagctt
     8281 tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa ctttggcgga
     8341 cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa cgggttgctg
     8401 tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg cggtggttct
     8461 gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa tgaatgggaa
     8521 ggtctggtca tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt ggatatcggc
     8581 atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg cgaaagcgat
     8641 gtccgcgtca attttggcgg tgtcaccttc ttctccggcg accatcttta tgccgacaat
     8701 accgggatta ttctttctga agatccgctg gatattgaat gatagaaaag gcaccgcaag
     8761 gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc ggaagatgaa
     8821 gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct gccagccgtt
     8881 ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat gctgggcatt
     8941 ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt ccatctgcaa
     9001 cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa acacttctaa
     9061 tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaatc ctcgtcccga
     9121 ttaccggtga cgccttaata aatacgagcg cactttagtt agctccgatt gtatgaagcc
     9181 gcgccatcgc tgtccagcgg cacgccttgc agattacggt ttgccacact tttcatcctt
     9241 ctcctggtga cataatccac atcaatcgaa aatgttaata aatttgttgc gcgaatgatc
     9301 taacaaacat gcatcatgta caatcagatg gaataaatgg cgcgataacg ctcattttat
     9361 gacgaagcac acacatttta agttcgatat ttctcgtttt tgctcgttaa cgataagttt
     9421 acagcatgcc tacaagcatc gtggaggtcc gtgactttca cgcatacaac aaacattaac
     9481 tcttcaggat ccgattatga gtcaaacatc aaccttgaaa ggccagtgca ttgctgaatt
     9541 cctcggtacc gggttgttga ttttcttcgg tgtgggttgc gttgcagcac taaaagtcgc
     9601 tggtgcgtct tttggtcagt gggaaatcag tgtcatttgg ggactggggg tggcaatggc
     9661 catctacctg accgcagggg tttccggcgc gcatcttaat cccgctgtta ccattgcatt
     9721 gtggctgttt gcctgtttcg acaagcgcaa agttattcct tttatcgttt cacaagttgc
     9781 cggcgctttc tgtgctgcgg ctttagttta cgggctttac tacaatttat ttttcgactt
     9841 cgagcagact catcacattg ttcgcggcag cgttgaaagt gttgatctgg ctggcacttt
     9901 ctctacttac cctaatcctc atatcaattt tgtgcaggct ttcgcagttg agatggtgat
     9961 taccgctatt ctgatggggc tgatcctggc gttaacggat gatggcaacg gtgtaccacg
    10021 cggccctttg gctcccttgc tgattggtct actgattgcg gtcattggcg catctatggg
    10081 cccattgaca ggttttgcca tgaacccagc gcgtgacttc ggtccgaaag tctttgcctg
    10141 gctggcgggc tggggcaatg tcgcctttac cggcggcaga gacattcctt acttcctggt
    10201 gccgcttttc ggccctatcg ttggcgcgat tgtaggtgca tttgcctacc gcaaactgat
    10261 tggtcgccat ttgccttgcg atatctgtgt tgtggaagaa aaggaaacca caactccttc
    10321 agaacaaaaa gcttcgctgt aatatgacta cgggacaatt aaacatgact gaaaaaaaat
    10381 atatcgttgc gctcgaccag ggcaccacca gctcccgcgc ggtcgtaatg gatcacgatg
    10441 ccaatatcat tagcgtgtcg cagcgcgaat ttgagcaaat ctacccaaaa ccaggttggg
    10501 tagaacacga cccaatggaa atctgggcca cccaaagctc cacgctggta gaagtgctgg
    10561 cgaaagccga tatcagttcc gatcaaattg cagctatcgg tattacgaac cagcgtgaaa
    10621 ccactattgt ctgggaaaaa gaaaccggca agcctatcta taacgccatt gtctggcagt
    10681 gccgtcgtac cgcagaaatc tgcgagcatt taaaacgtga cggtttagaa gattatatcc
    10741 gcagcaatac cggtctggtg attgacccgt acttttctgg caccaaagtg aagtggatcc
    10801 tcgaccatgt ggaaggctct cgcgagcgtg cacgtcgtgg tgaattgctg tttggtacgg
    10861 ttgatacgtg gcttatctgg aaaatgactc agggccgtgt ccatgtgacc gattacacca
    10921 acgcctctcg taccatgttg ttcaacatcc at
//
LOCUS       DAEVZA010000150        10840 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEVZA010000150 DAEVZA010000000
VERSION     DAEVZA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.150"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..539
                     /gene="tmcA"
                     /locus_tag="LAF92_004235"
     CDS             <1..539
                     /gene="tmcA"
                     /locus_tag="LAF92_004235"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBI9793156.1"
                     /translation="FGYTGELWRFWHRCGFVLVRMGNHREASSGCYTAMALLPMSNAG
                     KQLAEREHYRLRRDAQALAKWNGETLPVDPLNDAVLSDDDWLELAGFAFAHRPLLTSL
                     GCLLRLLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLVRQREEAAQALFALN
                     NVRTERLRDRITQWQFFH"
     gene            613..1311
                     /gene="ypfH"
                     /locus_tag="LAF92_004236"
     CDS             613..1311
                     /gene="ypfH"
                     /locus_tag="LAF92_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI9793157.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(1421..1621)
                     /locus_tag="LAF92_004237"
     CDS             complement(1421..1621)
                     /locus_tag="LAF92_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBI9793158.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1649..2776)
                     /gene="dapE"
                     /locus_tag="LAF92_004238"
     CDS             complement(1649..2776)
                     /gene="dapE"
                     /locus_tag="LAF92_004238"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBI9793159.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMSRNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(2780..3136)
                     /locus_tag="LAF92_004239"
     CDS             complement(2780..3136)
                     /locus_tag="LAF92_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBI9793160.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            3517..3576
                     /gene="ypfM"
                     /locus_tag="LAF92_004240"
     CDS             3517..3576
                     /gene="ypfM"
                     /locus_tag="LAF92_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBI9793161.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(3675..6788)
                     /gene="acrD"
                     /locus_tag="LAF92_004241"
     CDS             complement(3675..6788)
                     /gene="acrD"
                     /locus_tag="LAF92_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBI9793162.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(6952..8652)
                     /gene="narQ"
                     /locus_tag="LAF92_004242"
     CDS             complement(6952..8652)
                     /gene="narQ"
                     /locus_tag="LAF92_004242"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBI9793163.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            8858..10837
                     /gene="aegA"
                     /locus_tag="LAF92_004243"
     CDS             8858..10837
                     /gene="aegA"
                     /locus_tag="LAF92_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBI9793164.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
BASE COUNT         2651 a         2814 c         2812 g         2563 t
ORIGIN      
        1 gttttggtta caccggggag ttatggcgtt tctggcaccg ctgcggtttt gtgctggtgc
       61 ggatgggtaa tcatcgtgaa gccagcagcg gttgctatac ggcgatggcg ctgttaccga
      121 tgagtaatgc gggtaaacaa ctggctgaac gtgagcatta ccgtttacgt cgcgatgcgc
      181 aagctctcgc aaagtggaat ggcgaaacgc ttcctgttga tccactaaac gatgccgtcc
      241 tttctgacga cgactggctt gaactggccg gttttgcttt cgctcatcgt ccgctattaa
      301 cgtcgttagg ttgcttattg cgtctgctac aaaccagtga actggcatta ccggcgctgc
      361 gtgggcgttt acagaaaaac gccagtgatg cgcagttatg taccacactt aaactttcag
      421 gccgtaagat gttactggtt cgccagcggg aagaggccgc gcaggcgctg ttcgcactta
      481 ataatgttcg cactgagcgt ttgcgcgatc gcataacgca atggcaattt tttcactgac
      541 tccttcaatt ttctcccatg gtcattgtga gtcagcggcg taaaattaat gacatcagtt
      601 aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa gcccggataa acctgcgcaa
      661 cagttgttac tgcttttcca cggtgtcggg gataacccgg tggcaatggg ggaaataggc
      721 agctggtttg cgccgctatt tcctgatgcg ctggtggtga gtgtcggagg cgcggagccg
      781 agcggtaatc cggcggggcg tcagtggttt tcggtgcagg gtattacgga agataatcgc
      841 caggcgcgtg tggatgccat catgccgacg tttattgaaa cggtgcgcta ctggcagaaa
      901 cagagcggag taggggctaa cgccacggcg ctaatcggtt tctctcaagg cgcgattatg
      961 gcgctggaga gcattaaagc cgaaccgggc cttgcttcac gcgtcattgc ttttaacgga
     1021 cgctatgcga gcctgccgga aaccgcgtct acagccacga caattcactt gattcatggt
     1081 ggtgaagatc cggttatcga tctggcgcat gcagttgcgg cgcaggaagc gttaatcagt
     1141 gccggtggtg acgtgacgct ggatatcgtg gaggatttag gccatgcgat cgacaatcgc
     1201 agtatgcaat ttgccctgga tcacttgcgt tataccattc cgaaacatta tttcgacgaa
     1261 gcgctaagtg gcggtaagcc tggcgatgat gatgtgattg aaatgatgta aaagacagat
     1321 tacctggcct gataagacgc gtgagcgtcg catcaggcat cgttcaccat tgccggatgc
     1381 ggcgttaacg ccttatccgg cctacggttg agcactcagc ttacttcttc ggttgatcct
     1441 ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa atcacgatgc gggggaagct
     1501 ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt gagatcttta atcacgttca
     1561 gcaggacgcc taccaaaaag acaatcacca gaatccacca atatttagcc agccagtcca
     1621 tgcttatttc ctcttgcaga accactcatc aggcgacgag ctgttccatg atacgttgat
     1681 acatacgggc aagtagctgc aggtcggcag cgttcacaca ttcattaatt ttatgaatag
     1741 tggcattgac cggcccgagt tccaccacct gcgcccccat gcgagcaata aagcgcccgt
     1801 cggacgttcc gcctgtggtc agtagctgcg gtttaatttc attatagtgc tcaaccgcgt
     1861 taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa tggctgcccg gaaagccacc
     1921 agtccaccgt ataacgcagt tgatgttttt caagcagggc aagcacctgt gctttgatca
     1981 tctcatcagt cagttcggtg ctgaagcgga agttaaactg cacaaacagt tcacccggaa
     2041 taacgttgtt actgcccgtt cccgcctgaa tattggcaat ctgcatactg gtcgccggga
     2101 agaattcatt gccctgatcc cactcaatag ccactaattc attaaggaaa ggtgctgcgc
     2161 gatgtaccgg attgtcagcc agatgcgggt aggcaacatg cccctgaacg ccatgaatgg
     2221 tgaggttgca ggttaacgat ccgcgacgac catttttcac cacatcacca accacttcga
     2281 tactcgacgg ttcgccgacc aggcagtaat cgagacgctc attacgcgac attaacgctt
     2341 cgacgacttt taccgtaccg ttgtgggcac tggcttcttc atcagaggtg atcagaaatg
     2401 ccagtcgccc cgtatggttg ggatgttgtg cgacaaagcg ttctgccgct accaccatcg
     2461 ccgccagcga gcctttcata tctgccgcac cgcgtccgaa taacatgcca tcacgaatgg
     2521 tgggttcaaa cggcggattg atccaacgat cggcgtcgcc aggcggcacc acgtcggtat
     2581 gcccggcaaa ggctaacgtt tcaccttgcc cacgccatgc ccaaaaattc tgcgtatcgg
     2641 caaagtccat gcgttcaacg gtaaaaccga tcgcctgcaa acgttcaatc aacaaagcct
     2701 ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat aagctgttgt gtcagctcaa
     2761 taaccgggca cgacatagac tacacctcat ggaaaaattg ctgataactg gaatcactga
     2821 aacccagcag cataggctta ccaggcgcgc agagcaatgg acgtttgata attgcaggca
     2881 tttcagtcat taatgccgcc gcagaggccg catcggtgat tttattgcgg gtggtttcgt
     2941 ccagtttacg ccaggttgta ccacgggtgt tcagtaacgc ttcccagcct aattcgttga
     3001 taaaaccgtt caataattcg ctgtccagcc catcgacgcg gtaatcatga aaacgatagt
     3061 cgatgttatt ggcttccagc caacggcgag cctttttaat ggtgtcacaa tttttgatgc
     3121 cgtaaagtgt aaccataatg aatccttttt cgatttatga gaataaatac tcatttaagg
     3181 gtgaaaacat tttgcctgca aatgattatc aggaaatata acattatcga agtattcaaa
     3241 ttaacaacta ttaaataata ttaaacattt ttaacgtaaa cattaagaat attcatcttg
     3301 ttatctgtct gtattttaac cagaattaaa tcagtattag atgccgtatg aatatctcga
     3361 aatgttgctg tgtatcacat acaaattatc accgcaggac catagtattt acataaggtt
     3421 tgtcccccgg aggtagtcat cgcagcaatc gagtaaatta gcttcaccac ggtatattgt
     3481 ttccgtagaa tacccataat tataagagag gttgttatga ttgaacgtga actggggaac
     3541 tggaaagact ttatcgaagt tatgcttcgt aagtaattcc cgagacaaag tataaacagc
     3601 aagaacccgc tgagcaggtt cttaatcgga aagttgaaaa aggcgacaca ttggcatgtc
     3661 gcctttttta ttgcttattc cgggcgcggc ttcagcggga agcggcggcg caccagcaca
     3721 aagaacagcg gcacgaagta aatagccaga atagtagctg aaatcatccc gcccattacg
     3781 ccagtaccca ccgcatgctg accaccggaa ccggcacccg tgctggttgc cattggcaat
     3841 acgccgaaga taaatgccag cgaggtcatc agaatcgggc gtaaacgctg acggcaggcg
     3901 tggagcgtcg cttcaaacag gtcgtggcct ttttggttca tctcgttagc aaactcgacg
     3961 atcaggatgg cgtttttcgc cgataaacca atgaccgtta acaggcccac ctggaagtaa
     4021 acgtcgtttt ccagcccgcg catccaggtt gccagcagcg cgccgattac ccccagcggc
     4081 acgaccagca ttaccgagaa cggtaccgac cagctttcat acaatgccgc tagacacagg
     4141 aatactacca gcaaggaaat ggcgtacagc gccggagcct gcgcgccgga aagccgctcc
     4201 tgatacgaca tcgccgtcca ctccagacca aagccgtttg gcaactgctt cactaacgat
     4261 tccataatat ccatcgccgt accggtactg acccccggtg cggcttcccc aacaatctca
     4321 accgcagaat aaccgttata gcgttccaga cgcggcgagc ctgtctccca gcgtgaggtc
     4381 gcgaaagcag agaagggcac catgccgcca tctttatttc ggacatacca gagattaatg
     4441 tcatctggca gcatgcgata cggcgcagct gcctgcacat agactttctt cacgcgaccg
     4501 cgatccataa agtcattaac atagctcgaa ccccaggcgg tttgcagtgt gtcgttaata
     4561 tcgtcgatat caacgcccag cgcctgagct ttacgctggt cgatatcaat ctgcaactgc
     4621 ggactgtcat cgaggccgtt atggcgcaca cgggttagct ccgggttttc cgccgccagc
     4681 gccagcaact ggttacgtgc tgccatcagc gcatcgtgac ctgctccagc gtggtcctgc
     4741 aactccatat caaaacctgc agaactacca agaccgctaa ttgctggcgg gctgctggcg
     4801 ataacgcgag cttctttaat cttgttaaac gccttcgttg cacgctcgat aatggcaaat
     4861 gaggtgccgg tcttactgtc gcgttcgctc cagtctttca ggcggataaa cattcgcgcc
     4921 acgttttgcc cgttaccccc ggggccagaa ccaacagtgg caaacaccga catgatgttg
     4981 tctttttcat gggtgaagta atatttctcg atttgctcaa cgactttcag ggtctgttgt
     5041 tgggttgaac cgctgggcaa ttgtaccgag gtagtaaaca tgccacggtc ttccagcggt
     5101 aagaacgacg tcggcaaacg caggaacagg aacaccatgc cgccaagcag caggacataa
     5161 atcacaatcc agcgcaggct acggtggaga attttcgcca cccctttttc gtagcgttcg
     5221 gcgttgcggt taaacatctg gttaaaccag gcaaaaaagc ctttttgccc gtgatgctca
     5281 ccttttttca gtggcttaag tagtgtggca cacagagccg gagtgaggat catcgctacc
     5341 agtactgaca gcaccatcgc cgcaacaatg gtaatagaga actggcgata gatggcaccg
     5401 gtggtgccgc cgaagaaggc cattggtaca aataccgccg aaagaaccat cgcaatcccg
     5461 accagtgccc cctggatctg ccccatcgat ttacgtgtgg cttcgcgagg agtgagtcct
     5521 tcctcactca taatacgttc gacgttttcc accaccacga tggcgtcatc caccagcaga
     5581 ccgatcgcca gcaccatcgc gaacatggtt aaggtgttga cgctgtaacc gaaggcgtaa
     5641 agtacggaga aggttcccat caacaccacc ggcacggcga tagtgggtat cagcgtggcg
     5701 cggaagtttt gcaggaacag atacataacg aggaaaacca gagcgatagc ttccagcagc
     5761 gttttcacca cgtcttcaat ggaggcttta acaaacgagg tggtttcata cgccaccttg
     5821 tattccagtc cgtgcgggaa atactgcgcc agctcgtcga gacgattgag caccagctcc
     5881 gctgtcgcca tttcgtttgc gccggaggcc agttttaccc ccagcccgga ggctggctta
     5941 ccattgaagc ggctaagata atcgtatttc tccgccccca tttcgacggt ggcgacatcg
     6001 cccagcctta cctctgagcc gtcttgattc accctcaagg tgatatcgcg gaactgttct
     6061 ggtgtttgca gcagtgactg ggcgttaatg gtggcgttga gcgcctgctt atcgacggaa
     6121 ggtgtaccac caagttgccc aaccgcaatc tgcgcgttct gtgactcaat ggcatcagtg
     6181 acatctttag ccgtcatctg gaaactgttg agtttcgccg ggtccagcca gatacgcatg
     6241 gaatattgcg aaccataggc atcgatatcc ccgacgccat tcacgcggct taacgggtcc
     6301 tgaatattac tggcaacata atcggcaata tcctgtttat ccatcgaacc atcggtagag
     6361 acgaaagcaa tggtcagaat attggtatcg ccggttttac gcaccgtcac gccctgattt
     6421 tgcaccgcct gcggtaactt tcgcatggct gattgcagct ggttttgtac ttgctgcacg
     6481 gcttcgtccg gatcggtgcc tgctttaaaa cttaaagtga cagatgcctg accggtgcca
     6541 ctgctctgag atgacatata catgagatta tcgaggccgg tcatattttg ctcgataacc
     6601 tgggtcacgg tgttttccag cgtctgggcc gatgcgccgg gatagttagc ggtcactcgc
     6661 acattcggtg gcgcgagatc ggggtattgt tcaacgggca atgaaaaaat cgccagggtc
     6721 cctgtcagac acaacaggat tgccagcacc caggcaaaaa tggggcgatc aataaagaaa
     6781 ttcgccatta aaagaggacc tcgtgtttct gcgtatcgtt gtaggcttga cttgcttcac
     6841 tgtagcggca aggtacgagc caatcgtgga gaaaaaaagg agttaatgta aattatcatg
     6901 cccttttata ctgaacctta agtgcaagta ttctttggtc agtaggaggc attacatcaa
     6961 ctgactttcc tcaccctccg cagagcgaaa gctaatactc actaacgtgc cgccgccgga
     7021 aggttgtgaa aaagtcagcg tcccacccag ccgttccgcg cgttcgcgca tgatattcag
     7081 accataatga ccttcgggct ctttcggttc accgatgcca atcccgttat cacggatata
     7141 aaccgtgtga ttgccgtccg gcgcggtgac gcaactgacg gcgatttcgc tggcgttggc
     7201 gtgcttcatc gcattcagca ccgcttcgcg aataatttgc aacaaatgca cctgcatttg
     7261 cgcatccagc gccagggttg gcagacggca gtcgagggtc agtttggcgc tggtttgatt
     7321 ttgtaacgta tccagcattt ccctcaacgc ggaggggaga tccgcctgct gcagcgtcag
     7381 gcggaaagta gtcaacagct cgcgtaactg ccgataagca tcattcaacg cctgggaaaa
     7441 atcggccatg atactttgtg cggtggcgtt atcttccggt atcgaacgct tcagtaacgt
     7501 caactggata cgtaagtaag aaagtacctg agccagcgag tcgtgcaatt cgcgggcgat
     7561 ggttgcacgt tcttccatca acaataactg ctgaaaatgc ttctgcgcct gattaaagta
     7621 caaaccgcgt cccagcatcg acgaaacgct gttaagcagc ggttctgatg atgaaacgtg
     7681 actattttgc cagtgcagtt cgccgtaaac cgtctcttgc attgtcaccg gtaaaatctg
     7741 catcggcaat tccgggtttg gttgcccttc gctaatccgc cagttttcac cgacatttaa
     7801 ctccagatat tcagccgctt cattgtcgcg aacaatctgc aaaatatggc ggaaacaatg
     7861 cacatcaatc tggctggtgt tcagcgcctg ggaacattga tacaacacct ccagacgacg
     7921 cttggcctcg tggagatcgc gggtcttttc ttctactgac gcctccagcg aaaggtacaa
     7981 tttatgcagc tcgctcgaca tctggttaaa ggtttttgca agcagaccaa gctcattcgg
     8041 cagactggta tccagcggcg gcgagtcgaa ctgcccgtgt tcaatacgtt gactggcggt
     8101 aaccagctga ttcagcgggg caaccacctg atggcgtata cggcgcaaag taaaaaagac
     8161 cagcgtgaaa ataccgatac cgccagccag ggaaatcgcc accaccagca gcattttgcg
     8221 ttcagcgtag tgctgtaaag ccagcacgaa caggtctatc tgattaacat aattattaat
     8281 attggcctga taccacggca aatcgccctt gctgagccga ttattcatct ccagccagtt
     8341 ggcattcaga tgcgcatagc gagtttttac tgcttctggc acataccaga cgttgagatt
     8401 ggttaatacc ggtgaatgca gtgcctgctg aaacaactgg cgatgtgcat tgagttgtgg
     8461 actgccactt tgcaagtcgt agcccaggcg gtaactctgc atacgtagcg atccggcaat
     8521 attgatagcc tcagcgtcgc gcaaactgct cgccagagtt agcagagcga tacccgtgga
     8581 aagaatcgac agcagcacaa tgtaaaaaaa ggcccgggcc agactggccg agacgggtcg
     8641 tttaacaatc acacgcgtct tctccacaaa aaatcattaa tgttccagtt agctgaaaca
     8701 caatttagtc agtgatggga ttatttgatc tgccacagtt tatagcgaaa tagttactta
     8761 ttctgggcac atgaacattg ccgattcccc tgtaagtcgg gtaataacaa cactcatata
     8821 aagaataagg tttttacaac caaaaaagaa ggtcgttatg aatcgtttta ttatggccaa
     8881 cagtcagcaa tgtctgggtt gtcatgcttg tgaaatcgcc tgtgtcatgg ctcacaatga
     8941 tgagcaacat gtcctgagcc aacaccattt tcatccccga attacggtta tcaaacatca
     9001 acagcaacgt agtgcagtga cctgtcacca ttgtgaagat gcgccctgcg cccgtagctg
     9061 ccctaatggc gcaatcagcc acgttgatga cagcattcag gtcaatcagc aaaagtgtat
     9121 tggctgtaaa tcctgcgtgg tggcctgtcc ttttggtacg atgcaaatcg tcctgacacc
     9181 cgtcgcggca ggaaaagtaa aagccacggc gcataaatgc gacctttgtg cggggcgcga
     9241 aaacggtcct gcctgtgttg agaattgccc ggcggacgcg ctgcaactgg tcactgacgt
     9301 cgcactctcc ggcatggcga aatcccgccg cttgcgcacc gcgcgtcagg aacatcaacc
     9361 gtggcatgcc agtaccgcgg cgcaagaaat gccggtaatg agtaaagtcg aacaaatgca
     9421 ggcaacgccc gcgcgtggcg agccggataa actggcgatt gaagcgcgca aaaccggttt
     9481 tgatgaaatt tatctgccat ttcgcgccga ccaggcacaa cgggaagcct cgcgctgcct
     9541 taagtgcggc gagcacagcg tttgtgaatg gacctgcccg ctgcataacc atattccgca
     9601 gtggattgaa ctggtgaaag ccggaaacat cgacgccgcc gtcgagcttt ctcaccagac
     9661 caacaccctg ccggaaatta ccggacgcgt ttgtccgcaa gaccgtttgt gtgaaggtgc
     9721 ctgtactatt cgcgatgagc acggtgctgt aactatcggc aacattgaac gctatatttc
     9781 agatcaggcg ttggcgaaag gttggcgtcc tgacttaagt catgtcacca aagtggacaa
     9841 gcgggtggcg attatcgggg caggcccggc agggctggcc tgtgcggatg ttctgacccg
     9901 caatggcgtg gcggtgacgg tgtacgatcg ccatccagaa attggtggct tgctcacttt
     9961 cggcattcct tctttcaaac tggataaatc cctgctggca cgccgtcggg aaatcttcag
    10021 cgcgatgggg attcacttcg aactcaattg tgaagtgggt aaagatgtct ctttggattc
    10081 gcttttggaa caatacgacg cggtcttcgt tggcgtaggc acttaccgtt ccatgaaagc
    10141 gggtttaccc aatgaagatg cgccgggcgt ttatgacgcg ctgccgttcc tcattgccaa
    10201 cactaaacag gtgatggggc tcgaagagct accggaagag ccgtttatca ataccgccgg
    10261 acttaacgtc gtggtactgg gcggcggcga caccgcgatg gactgtgtgc gtactgcgct
    10321 gcgccacggc gcgagtaacg tcacctgcgc ttatcgtcgt gatgaagcta acatgccagg
    10381 ctcgaagaaa gaggtgaaga acgcccgcga agagggggcc aacttcgaat ttaacgtcca
    10441 gccggtggcg cttgagctga atgaacaagg tcacgtttgc ggaattcgtt tcctgcgcac
    10501 gcgtcttggc gagccggatg cccaggggcg tcggcgacct gtaccgattg agggcagtga
    10561 atttgtcatg ccagccgatg cggtgattat ggcgtttggc ttcaatccac acgggatgcc
    10621 gtggctggag tcgcacggtg taacggtaga caaatggggc cgcatcatcg cggatgtgga
    10681 aagccagtac cgttaccaga ccaccaatcc gaaaatcttc gccggtggcg acgccgtgcg
    10741 tggtgcggat ctggtggtta ccgcaatggc agaaggacgt catgcggcac aggggattat
    10801 tgactggctg ggggtaaaat cagtcaaatc tcactgatag
//
LOCUS       DAEVZA010000151        10682 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEVZA010000151 DAEVZA010000000
VERSION     DAEVZA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.151"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1..2509
                     /locus_tag="LAF92_004244"
                     /pseudo
     CDS             1..2509
                     /locus_tag="LAF92_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(2509..3878)
                     /locus_tag="LAF92_004245"
     CDS             complement(join(2509..3398,3398..3878))
                     /locus_tag="LAF92_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HBI9793165.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            3920..5458
                     /locus_tag="LAF92_004246"
                     /pseudo
     CDS             3920..5458
                     /locus_tag="LAF92_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(5720..6772)
                     /locus_tag="LAF92_004247"
                     /pseudo
     CDS             complement(5720..6772)
                     /locus_tag="LAF92_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
     gene            7087..8403
                     /gene="shiA"
                     /locus_tag="LAF92_004248"
     CDS             7087..8403
                     /gene="shiA"
                     /locus_tag="LAF92_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBI9793166.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGTLIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            8505..9959
                     /gene="amn"
                     /locus_tag="LAF92_004249"
     CDS             8505..9959
                     /gene="amn"
                     /locus_tag="LAF92_004249"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBI9793167.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            10302..>10682
                     /locus_tag="LAF92_004250"
     CDS             10302..>10682
                     /locus_tag="LAF92_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI9793168.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSV"
BASE COUNT         2862 a         2512 c         2467 g         2841 t
ORIGIN      
        1 ggctaacagc aatgtcagcg atgcacagcc aatgacgttt gtggcggata aagacagagc
       61 ggttgtcgtt ttgcaaacat cgaaagcgga aatcattggg aatggcgtgg atgagacaac
      121 tctgacagca acagtgaaag atccgtcgaa tcatccggtg gcggggataa cggtaaactt
      181 caccatgcca caggacgttg cggcaaactt tacccttgaa aataacggta ttgccatcac
      241 tcaggccaat ggggaagcgc atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt
      301 taccgcaacg ctgggtaata acaataccag tgattcgcag ccggtaacat ttgtggcgga
      361 caaagcctcg gctcaggttg tcctgcagat atcaaaagat gagatcacag gtaatggcgt
      421 cgatagcgca acgctaactg caacggttaa agatcagttc gacaatgagg tgaataatct
      481 tccggtaaca ttcagctcag cctcttcagg actcaccctg accccgggag taagtaatac
      541 caacgagtct ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agaagacggt
      601 tactgcatca ctggctaata atggtgccag cgacaacaaa actgtgcatt ttattggcga
      661 cacagcggcg gcaaaaatta tcgagttgac gcctgtccca gacagcataa tcgccggtac
      721 cccgcagaac agctccggca gcgtcatcac cgccacagtc gttgataata atggctttcc
      781 ggtgaaaggt gtgactgtga acttcaccag caacgcagcg acagccgaaa tgacgaacgg
      841 cggtcaagcc gtgacgaacg aacagggtaa gactaccgtc acttatacca atacccgctc
      901 ctcgatagaa tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc
      961 cacgcttagc acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcaccttgct
     1021 acaggcactt tttgatacag tctccgcagg cgagacaacc agtctgtata ttgaggtgaa
     1081 ggataattac ggcaacggtg tcccccagca ggaggtaacc ctcagcgttt caccaagtga
     1141 aggcgtgacc cccagtaata acgctatata tactaccaac cacgacggca atttttacgc
     1201 aagctttacc gctacaaaag ccggggttta tcaattgacg gcaaccctcg aaaatggcta
     1261 ttcgatgcaa caaacagtga cctatgtgcc gaacgtcgcg aatgctgaaa tcacgctggc
     1321 agcctcgaag gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt
     1381 cgctgataca gagggcaatg cgatagccaa cactgaggta acatttactc tgccggaaga
     1441 tgtgaaggcg aacttcacgc tgagcgatgg cggtaaagtg attactgatg ctgaaggcaa
     1501 agcgaaagtc acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgatgac
     1561 tggcggtaag agtgagcagt tggtggtgaa ctttattgcg gatacgctca ctgcgcaggt
     1621 taatcttaac gttaccgagg acaattttat cgctaataac gtcgggatga ccaggctgca
     1681 ggcaacagtg actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct
     1741 accggcagat gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgatat
     1801 caacggcaag gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt
     1861 tagcgtgaac aattatggtg tcagtgatac gaaacaggtg actttgattg ccgatgctgg
     1921 taccgcaaaa ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaggg
     1981 cgcaaccatg acggcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt
     2041 taatttccgc ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg
     2101 tttcgctgaa attcttgtga caagcaccga ggtcggactg aaaacagttt cagcctctct
     2161 ggcagataaa cctactgaag tcatctcgcg attactgaat gccagtgcag atgttaattc
     2221 tgcgacgatt accagtctgg agataccgga aggtcaggta atggtcgcac aagacgtagc
     2281 agttaaagct cacgttaacg accagtttgg caacccggtt gcgcatcaac ccgtgacatt
     2341 cagtgcagag ccatcctcgc aaatgatcat cagccagaat acggtctcta ctaatacgca
     2401 gggtgtagcc gaggtcacca tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc
     2461 cctgccgaat ggagcctcac tttgtactga ccccaaaaag ttggacagtt aaacacgagg
     2521 catataggtc tgattccgat attcaattgg agtcagacct tttaatttca ggctaattct
     2581 tctgctgttg tagtattcaa tatattccgt aacagcatcc ttcagttcgc ttatattact
     2641 gaactcatca agataaaaac actccgactt taaggttcca aagaaacact ccaccacagc
     2701 attatccaga caattgcctt ttctggacat gctttgttta ataccatgtt ctttaaggat
     2761 attttgatat cttctcatac gatactgcca tccctggtca gagtgcagaa caggatgctc
     2821 gtgaggatta agctttttga atgcctgatc gagcatattc tcaaccatgt tcatcactgg
     2881 tctttccgaa aggctgtaag aaataacttc gttgttgaag agatctatta ctggagacaa
     2941 atacagcttg cgcccattga ctgcaaattc agtaacatcg gtaacccact tctcgtttgg
     3001 ccgcgtagcc ttgaaatctc tttggagaac attaggggcg gtttgcccta cctctcctct
     3061 gtaagagcgg tatcgcttga ccttaatcgc tgctttaagt gagagggttc ccatcaggcg
     3121 ctgaacagct ttatggttaa tctgtttccc ttctcgatga agagacagcg ttaccctacg
     3181 gtatccgtat cggcctctat tctcgtgata aatctcacta atacgctttt taacgtccgc
     3241 atacttgtca ggcttgctga gagcctttag atgataataa aacgtactgc gcggtatctc
     3301 cgcagccctg agaagctcat caagaggata aaactgcctt agctcgttga gtactttcac
     3361 tttttcgtgg gatgagctaa ggctttcagc ttttttagat acataagccg cgtttcaaga
     3421 aatcgaactt gcctttcaag atcctcaatg cgtcggtctt ttgacagctc caatgctgat
     3481 gccgcttttt ctggatcaac tgatattgca atgtttcttt tggtgccaat cttgagcgcg
     3541 cgtaaaccag cttctccgcg ctcttcatag accttcagcc acctggctac agaaccacta
     3601 ccagcaagca taaagtgagc agcagcctga ttaagggaca tgtgctgctc gatcacagct
     3661 ttcacgacct taatacgcaa ctctggatca gcactaacgc ctttaggttt gggaattaaa
     3721 cctttttctc catgtttttc atagagggca acccatgtcc tgacctgggt tcgggggaca
     3781 ccaaaacgtg ccgagatgat cctgtaacca tcatcagttg tgaagtagtg attcacgact
     3841 tcaaggcgct tttcaaaagg gtattttggc tttgacatat taggggctat tccatttcat
     3901 cgtccaacaa aatgggtgca gtacacttga gaaacaactg gaggctattg atgaaaaact
     3961 gacactcacg gcgtccagtc cgcttatcgg tgtctatgcc cctacaggcg ctactctgac
     4021 ggcaacgcta acctctgcaa atggcactcc agtggagggt caggtcatca actttagcgt
     4081 aacgccagaa ggggcgacgt taagtggcgg aaaagtgaga actaactctt caggtcaggc
     4141 tccagtcgtt ttgaccagca ataaagtcgg tacatatacg gtgactgcat ctttccataa
     4201 cggcgtaaca atacagacac agacaaccgt gaaagtcact ggcaactcaa gcaccgccca
     4261 tgttgctagc tttatcgctg atccatcgac tatcgccgcc accaacactg atttaagtac
     4321 cttaaaggca acggttgagg atggcagtgg taacctgatc gaaggtctca ctgtgtactt
     4381 cgccttaaaa agcggctctg ccacattaac gtcattaaca gcggtgaccg atcaaaacgg
     4441 aatcgcgaca acaagcgtga aaggagcgat gacaggtagc gtcacggtaa gcgcagtcac
     4501 gaccgctggt ggaatgcaaa cagtagatat aacgctggtg gctggcccgg cagacacctc
     4561 gcagtccgtc cttaagagca atcggtcatc actgaaaggg gactataccg atagtgctga
     4621 attacgtctt gttctgcacg atatatcagg caatccgatc aaagtttctg aagggatgga
     4681 atttgtgcaa tcaggtacta acgtgcccta tataaaaatt agcgcaattg attacagtct
     4741 aaatatcaac ggtgattaca aagccactgt tacaggaggc ggagagggta tcgcaacgct
     4801 gatccctgta ttgaatggtg ttcatcaagc tggtctgagt accacaatac aattcactcg
     4861 cgcagaagac aaaataatga gcggtacagt atcagtcaat ggtactgacc taccgacaac
     4921 tacattccct tcgcagtggt tcaccggggc gtattatcag ttgaataatg acaactttgc
     4981 cccaggaaaa acggcggctg attatgagtt ttcaagctct gcctcctggg tcgatgttga
     5041 tgctaccggt aaagtgacat ttaaaaatgt cggcagcaat tcggaaagga ttacggcgac
     5101 gccaaaatca ggaggcccta gctatgtata cgaaatccgt gtgaagagtt ggtgggtgaa
     5161 cgccggcgag gctttcatga tatacagcct tgctgaaaat ttttgcagca gcaatggcta
     5221 cacgctcccc agagcaaact atttaaacca ctgtagttcc cgaggcatcg ggtcactgta
     5281 cagtgaatgg ggagatatgg ggcattacac gactgacgct ggttttcaat caaatatgta
     5341 ttggtcatct agtcccgcaa actcaagcga acaatacgta gtttccctgg caacaggtga
     5401 tcaaagcgta tttgaaaagc ttgggttttc ttatgcgaca tgttataaaa acctgtgatt
     5461 ttccgttata atttcttaaa gagtgttcca ttcggggcac tcttttttac attccctcta
     5521 cagaactagc ccttctctca cagaaaatga ataaataaaa atgcggcacc gccagaatcg
     5581 cgttcgatgt gactatgatc attttcggtg agtactgctt tgatctaaag gctggttttc
     5641 tgtgatgatt gaccagcctc agagtattga tgacaccacc gttcataaca gatcggcatt
     5701 actatgcata agtactgtct catattctcg ccaaaacact ttttaatgcc tttgcgaatt
     5761 catccagact tatattgtca attttttgcc caacgacgac tgactccgag acatcggcca
     5821 ttgcgggcca tcgcgttttg taatcagcat aaattgcaac aacaggggta ttataggcac
     5881 ttgccatatg taatattgag gtgtcaggag ttattgcgat ataagcatct tttatgattg
     5941 ctgctgaacg tttaatcgag ggtggtaagg ataaacggta gacattatta taacaatcca
     6001 ctaatgccct ggctttatct tcacctttgg gtccatgaac gatcactatt ggtatatctg
     6061 tttcacttta aatttttgct attagatttt ctgcaatcga taatgaaaat gtacgttcct
     6121 gcgagctacc ttctaaattg aacgcaatgt aagatcctaa cgatcgcatt tcctcgcgca
     6181 tctcatcgag tacatcctca cttagtggca attcatatat tggcctgact gctggaaaac
     6241 ccgcctcacg catcataaat gcccatgtca taggtacggg agcccggagt ttctgatcca
     6301 tacgggacgc gttcttgcac aaaggggaga agcaattcat ggttatacca acaacctgaa
     6361 aattcgtttt tgctttcaac tgactgataa ataacatcgt tttcaggttc tttttacgca
     6421 tcccctcaat gcaaagatcc ggcgtaccgt attgctgtgt tatgttcttt gctaaatctt
     6481 ttatttcttt taatgttgcg tgatcctgca tagtcattgt gactaatgtt aatttagtct
     6541 gttcaagttt aagcgcatta aagacttcta aattaattgt cgacgttaca attaaaagat
     6601 gcttaatttt atgcaattca agcgcccgaa taacaggaaa gatggccata gcatcgccaa
     6661 tctgatcggg aatatggatg acaacaaagt ctgttttttc aatattgaaa ttataagctt
     6721 tataatcgta gtaactaaat gcaatacgtc tcaacaatga tgctaaaaac atacctaacc
     6781 tcgcctccct actggttata atgcaatgca gtctatcaga ctcatcaggg tgccattttg
     6841 tgcatatgcg gacttttatg tttcatatct ctaacctgtg ggtcctctgc ttaatcctta
     6901 aacaacacca gcaactcctg cgctttcatc ttccatcgaa tttttcatgt tgccgctaat
     6961 cagccataaa atcatttgca gatgcgctct gtcgaggtag tctcataagg ttcgtttata
     7021 gatcgacggc aatgtgagtt accttttcca tactaattat aaaaagacag tacaaacagg
     7081 atcattatgg actccacgct catctccact cgtcccgatg aagggacgct ttcgttaagt
     7141 cgcgcccgac gagctgcgtt aggcagcttc gctggtgccg tcgtcgactg gtatgatttt
     7201 ttactctatg gcatcaccgc cgcactggtg tttaatcgcg agtttttccc gcaagtaagc
     7261 ccggcgatgg gaacgctcgc cgcatttgct acctttggcg tcggatttct tttccgtccg
     7321 ctcggcggtg tcattttcgg tcactttggc gaccgactgg gacgtaagcg catgttaatg
     7381 ctgaccgtct ggatgatggg catcgcgaca gccttgattg gtattcttcc ttcattctcg
     7441 accattgggt ggtgggcacc tattttgctg gtgacactgc gtgccattca gggatttgca
     7501 gtcggcggcg aatggggagg cgcggcgttg ctttccgttg aaagtgcacc gaaaaataaa
     7561 aaagcctttt acagtagcgg tgtacaagtt ggctacggtg taggtttact gctttcaacc
     7621 ggactggttt cattgatcag tatgatgacg actgacgaac agtttttaag ctggggctgg
     7681 cgcattcctt tcctgtttag catcgtactg gtactgggag cattgtgggt gcgcaatggc
     7741 atggaggagt ccgcggaatt tgaacaacag caacattatc aagctgccgc gaaaaaacgc
     7801 atcccggtta tcgaagcgct gttacgacat cccggtgctt tcctgaagat tattgcgcta
     7861 cgactgtgcg aattgctgac gatgtacatc gttactgcct ttgcacttaa ttattcaacc
     7921 cagaatatgg ggctaccgcg cgaacttttc cttaatattg gtttgctggt aggtggatta
     7981 agctgcctga caattccctg ttttgcctgg cttgccgatc gttttggtcg ccgtagggtt
     8041 tatatcacag gtacgttaat cggaacgttg agcgcatttc ctttctttat ggcgcttgaa
     8101 gcacaatcta ttttctggat agttttcttc tccataatgc tggcaaacat tgcgcatgac
     8161 atggtggtgt gtgtgcaaca accgatgttt accgaaatgt ttggtgccag ttatcgctat
     8221 agtggcgctg gagtcggtta tcaggttgcc agtgtggttg gcggtggatt tacacctttt
     8281 attgccgctg cactcatcac ttactttgcc gggaactggc atagcgtcgc catttatttg
     8341 ctggctggat gcctgatttc cgcaatgacc gctttgttga tgaaagacag tcaacgcgct
     8401 tgatagcctg gcgaagatca tccgatcttc gccttacact tttgtttcac atttctgtga
     8461 catactatcg gatgtgcggt aattgtatgg aacaggagac acacatgaat aataagggct
     8521 ccggtctgac cccagctcag gcactggata aactcgacgc gctgtatgag caatctgtag
     8581 tcgcattacg caacgccatt ggcaactata ttacaagtgg cgaattacct gatgaaaacg
     8641 cccgcaaaca aggtcttttt gtctatccat cactgaccgt aacctgggac ggtagcacaa
     8701 ccaatccccc caaaacgcgc gcatttggtc gctttaccca cgcaggcagc tacaccacca
     8761 cgattactcg ccctactctc tttcgttcgt atcttaatga acaacttacg ttgctgtatc
     8821 aggattatgg tgcgcatatc tcagtgcaac cctcgcagca tgaaatccct tatccttatg
     8881 tcatcgatgg ctctgaattg acacttgatc gctcaatgag cgctgggtta actcgctact
     8941 tcccgacaac agaactggcg caaattggcg atgaaactgc agacggcatt tatcatccaa
     9001 ctgaattctc cccgctatcg cattttgatg cgcgccgcgt cgatttttcc ctcgcacggt
     9061 tgcgccatta taccggtacg ccagttgaac attttcagcc gttcgtcttg tttaccaact
     9121 acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag ccagatcctc gatcctgata
     9181 gtccctacat tgccctttct tgtgctggcg ggaactggat caccgccgaa accgaagcgc
     9241 cagaagaagc catttccgac cttgcatgga aaaaacatca gatgccagca tggcatttaa
     9301 ttaccgccga tggtcagggt attactctgg tgaatattgg cgtgggaccg tcaaatgcta
     9361 aaaccatctg cgatcatctg gcagtgctac gcccggatgt ctggttgatg attggtcact
     9421 gtggcggatt acgtgaaagt caggccattg gcgattatgt acttgcacac gcttatttac
     9481 gcgatgacca cgttcttgat gcggttctgc cgcccgatat tcctattccg agcattgctg
     9541 aagtgcaacg tgcgctttat gacgccacca agctggtgag cggcaggccc ggtgaggaag
     9601 tcaaacagcg gctacgtact ggtactgtgg taaccacaga tgacaggaac tgggaattac
     9661 gttactcagc ttctgcactt cgttttaact taagccgggc cgtagcaatt gatatggaaa
     9721 gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc atacgggaca ctactgtgtg
     9781 tttcagataa accgttgcat ggcgagatta aacttcccgg ccaggctaac cgtttttatg
     9841 aaggcgctat ttccgaacat ctgcaaattg gcattcgggc gatcgatttg ctgcgcgcag
     9901 aaggcgaccg actgcattcg cgtaaattac gaacctttaa tgagccgccg ttccgataat
     9961 aagttctgaa ccttcttatc agaaatagat gtgaaggagt aagtaagaca cctggcaaat
    10021 agcctgcaat aatcgtgggc tgtttgcttc cttgggcgga tacgagtttt attatcgtct
    10081 taatgatttc cacatattaa aagcaagtat gctttcaaaa cacaattata aaaaatcccg
    10141 ccaacaatat aagtttttat aaaattaaat ataagattat ggctttagaa tatttttatt
    10201 tctaatagac gagatttttc ctgttatgat ataatatgct gaattaacac atgttaacga
    10261 tttaccagta atgtaaataa attttcgagg agatcattcc agtgggacgt aaatgggcca
    10321 atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc taaaatttat gcaaaattcg
    10381 gtgtagaaat ctatgctgct gctaaacaag gtgaacccga tccagaatta aacacatctt
    10441 taaaattcgt tattgaacgt gcaaagcagg cacaagttcc aaagcacgtt attgataaag
    10501 caattgataa agccaaaggc ggcggagatg aaacgttcgt gcagggacgt tatgaaggct
    10561 ttggtcctaa tggctcaatg attatcgccg agacattgac ttcaaatgtt aaccgtacga
    10621 ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa tatcggagcg gcaggttctg
    10681 tc
//
LOCUS       DAEVZA010000152        10674 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEVZA010000152 DAEVZA010000000
VERSION     DAEVZA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.152"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..254
                     /locus_tag="LAF92_004251"
     CDS             <1..254
                     /locus_tag="LAF92_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBI9793169.1"
                     /translation="TQAQVAQVLQQAYAHKPLVRLYDKGVPALKNVVGLPFCDIGFAV
                     QGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI"
     gene            262..1038
                     /gene="argB"
                     /locus_tag="LAF92_004252"
     CDS             262..1038
                     /gene="argB"
                     /locus_tag="LAF92_004252"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBI9793170.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            1099..2472
                     /gene="argH"
                     /locus_tag="LAF92_004253"
     CDS             1099..2472
                     /gene="argH"
                     /locus_tag="LAF92_004253"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBI9793171.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            2738..3655
                     /gene="oxyR"
                     /locus_tag="LAF92_004254"
     CDS             2738..3655
                     /gene="oxyR"
                     /locus_tag="LAF92_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBI9793172.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3638..5038)
                     /gene="sthA"
                     /locus_tag="LAF92_004255"
     CDS             complement(3638..5038)
                     /gene="sthA"
                     /locus_tag="LAF92_004255"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBI9793173.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5369..6019
                     /gene="fabR"
                     /locus_tag="LAF92_004256"
     CDS             5369..6019
                     /gene="fabR"
                     /locus_tag="LAF92_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBI9793174.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDIGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6019..6378
                     /locus_tag="LAF92_004257"
     CDS             6019..6378
                     /locus_tag="LAF92_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBI9793175.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6418..7518)
                     /gene="trmA"
                     /locus_tag="LAF92_004258"
     CDS             complement(6418..7518)
                     /gene="trmA"
                     /locus_tag="LAF92_004258"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBI9793176.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7632..7822
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7887..9731
                     /gene="btuB"
                     /locus_tag="LAF92_004259"
     CDS             7887..9731
                     /gene="btuB"
                     /locus_tag="LAF92_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBI9793177.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9676..10533
                     /gene="murI"
                     /locus_tag="LAF92_004260"
     CDS             9676..10533
                     /gene="murI"
                     /locus_tag="LAF92_004260"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBI9793178.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2475 a         2604 c         2954 g         2641 t
ORIGIN      
        1 tgacccaagc gcaagtcgcg caagtgttac agcaggcgta tgcccataaa ccgctggtgc
       61 ggctgtatga caaaggcgtt ccggcgctga aaaatgtcgt tgggctgcca ttttgcgata
      121 tcgggtttgc cgttcagggc gagcatctga ttattgtggc gaccgaagac aacttactga
      181 aaggcgcggc ggcacaagcg gtacagtgcg ccaatattcg tttcggctat gcggaaacgc
      241 agtctcttat ttaagggtgc aatgatgaat ccattaatta tcaaactggg cggcgtactg
      301 ctggatagtg aagaggcgct ggaacgtctg tttagcgcac tggtgaatta tcgtgagtca
      361 catcagcgtc cgctggtgat tgtgcacggc ggcggttgcg tggtggatga gctgatgaaa
      421 gggctgaatc tgccggtgaa aaagaaaaac ggcctgcggg tgacgcctgc tgatcagata
      481 gacattatca ccggagcact ggcgggaacg gcaaataaaa ccctgttggc atgggcgaag
      541 aaacatcaga ttgcggccgt aggtttgttt ctcggtgacg gcgacagcgt caaagtgacc
      601 cagcttgatg aagagttagg tcatgttgga ctggcgcagc caggttcgcc taagcttatc
      661 aactccttgc tggagaacgg ttatctgccg gtggtcagct ccattggcgt aacagacgaa
      721 gggcaactga tgaacgtcaa tgccgaccag gcggcaacgg cgctggcggc aacgctgggc
      781 gcggatctga ttttgctctc cgacgtcagc ggcattctcg acggcaaagg gcaacgcatt
      841 gccgaaatga ccgccgcgaa agcagaacaa ctgattgagc agggcattat tactgacggc
      901 atgatagtga aagtgaacgc ggcgctggat gcggcccgca cgctgggccg tccggtagat
      961 atcgcctcct ggcgtcatgc ggagcagctt ccggcactgt ttaacggtat gccgatgggt
     1021 acgcggattt tagcttaagt tttgttggcc ggaggcgcag ctttccggca ttgaatttca
     1081 aaataaggaa acagagttat ggcactttgg ggcgggcgtt ttacccaggc agcagatcaa
     1141 cggttcaaac aattcaacga ctcactgcgc tttgattacc gtctggcgga gcaggatatt
     1201 gttggctctg tggcctggtc caaagccctg gtcacggtag gcgtgttaac cgcagaagag
     1261 caggcgcaac tggaagaggc gctgaacgtg ctgctggaag atgttcgcgc caggccacaa
     1321 caaatccttg aaagcgacgc cgaagatatc catagctggg tggaaggcaa actgatcgac
     1381 aaagtgggcc agttaggcaa aaagctgcat accgggcgta gtcgtaacga tcaggtagcg
     1441 actgacctga aactgtggtg caaagatacc gttagcgagt tactgacggc taaccgacag
     1501 ctgcaatcgg cgctggtgga aaccgcacaa aacaatcagg acgcggtcat gccaggttac
     1561 actcacctgc aacgcgccca gccggtgacg ttcgcgcact ggtgcctggc ctatgttgag
     1621 atgctggcgc gtgatgaaag ccgtttgcag gatgcgctta agcgtctgga tgtcagccca
     1681 ttaggctgtg gcgcgctggc gggaacggcc tatgaaatcg accgtgaaca gttagcaggc
     1741 tggctgggct ttgcttcggc gacccgtaac agtctggaca gcgtttctga ccgtgaccat
     1801 gtgttggaac tgctgtcggc tgccgctatc ggcatggtgc atctgtcgcg ttttgctgaa
     1861 gatctgattt tctttaacac cggcgaagcg gggtttgtgg agctttctga ccgcgtgact
     1921 tccggttcat cattaatgcc gcagaagaaa aacccggatg cgctggagtt gattcgcggt
     1981 aaatgcggtc gggtgcaggg ggcgttaacc ggcatgatga tgacgctgaa aggtttgccg
     2041 ctggcttaca acaaagatat gcaggaagac aaagaaggtc tgttcgacgc gctcgatacc
     2101 tggctggact gcctgcatat ggcggcgctg gtgctggacg gcattcaggt gaaacgtcca
     2161 cgttgccagg aagcggctca gcagggttac gccaacgcca ccgaactggc ggattatctg
     2221 gtggcgaaag gcgtaccgtt ccgcgaggcg caccatattg ttggtgaagc ggtggtggaa
     2281 gccattcgtc agggcaaacc gctggaagat ctgccgctcg acgagttgca gaaattcagc
     2341 ccggtgattg acgaagatgt ctatccgatt ctgtcgctgc aatcgtgcct cgacaagcgt
     2401 gcggcaaaag gcggcgtctc accgcagcag gtggcgcagg caattgcttt tgcgcaggcg
     2461 cggttggggt aagaacattt atatgtataa atttgagcct ggcttatcgc cgggcttttt
     2521 tatggcaaaa aaagcggatc ctggagatcc gcaaaagttc acgttggctt tagttattcg
     2581 agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt aaaagaggtg ccgctccgtt
     2641 cctgtgagca attatcagtc agaatgcttg atagggataa tcgttcattg ctattcttcc
     2701 tatcgccatg aactatcgtg gcgatggagg atggataatg aatattcgtg atcttgagta
     2761 cctggtggca ttggctgaac accgccattt tcggcgtgcg gcagactcct gccacgttag
     2821 ccagccgacg cttagcgggc aaattcgtaa gctggaagat gagctgggcg tgatgttgct
     2881 ggagcggacc agccgtaaag tgttgttcac ccaggcggga atgctgctgg tggatcaggc
     2941 gcgtaccgtg ctgcgtgagg tgaaagtcct taaagagatg gcaagccagc agggtgagac
     3001 catgtccggg ccgctgcata ttggtttgat tcccacggtc gggccgtacc tgctaccgca
     3061 tattatcccg atgctgcacc agacctttcc aaagctggaa atgtatctgc atgaagcaca
     3121 gacccaccag ttactggcgc aactggacag cggcaaactc gattgcgtga tcctcgcgct
     3181 ggtgaaagag agcgaagcat tcattgaagt gccgttgttt gatgagccaa tgttgctggc
     3241 tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta ccgatggccg atctggcagg
     3301 ggaaaaactg ctgatgctgg aagacggtca ctgtttgcgc gatcaggcga tgggtttctg
     3361 ctttgaagcc ggggcggatg aagatacaca cttccgcgcg accagcctgg aaactctgcg
     3421 caacatggtg gcggcaggta gcgggatcac tttactgcct gcgctggctg tgccgccgga
     3481 gcgcaaacgc gatggggttg tttatctgcc gtgcattaag ccggaaccac gccgcactat
     3541 tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc tggcagaggc
     3601 catccgagca agaatggatg gccatttcga taaagtgtta aaacaggcgg tttaaaccgt
     3661 ttaacgcagc tacccgatag gcttccgcca tggtcgggta gttaaaggtg gtgttgacga
     3721 agtactcaat agtgttgccg ccacctttct gttccataat cgcctgaccg atatgaataa
     3781 tttcggcagc gcgctcgcca aagcagtgaa tacccagaat ctcttttgtt tcccgatgga
     3841 acaaaatttt cagcgtgccc acgttcatgc cgacgatttg tgcgcgtgcc agatgtttaa
     3901 actgggcgcg gcccacttca tatggcactt tcatcgcggt cagctgctgt tcggttttgc
     3961 ccacagagct gatttccggg atggtgtaaa tgccggtagg gatatcttca atcagatgcg
     4021 cggtggcttc gccttttacc agcgcctgcg cggcaatgcg cccctggtca taggccgctg
     4081 atgccaggct cggataacca atcacgtcgc ccaccgcgta aacgtgcggc tgtgcggtct
     4141 gatacatgct gttgaccttc agctgtccgc gactgtcagt ttccagccca atgttctgta
     4201 acgccagcga atcggtatta ccggtacgac cgttggcata gagcaggcag tcagctttca
     4261 gttttttacc cgacttcaga tgcatgatca caccgtcgtc acagccttcg atcttctcgt
     4321 actcttcgtt gtgacgaatc actacgccac tgttccagaa gtgataggag agagaatctg
     4381 acatctcttg atcgagaaat gccagcaggc gatcgcgggt gttgatcaga tccactttta
     4441 catccatacc gcggaagatc gacgcatatt cacagccgat cactccagca ccatagataa
     4501 gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc gctgtcgtaa atgcgtggat
     4561 gggtgaaatc aacatctgtt ggatgatatg gacgagagcc gcaggcaata acaaattttt
     4621 cagcggttag tgtttcaacg ctgccgtccg ggcaatccag cgccaacgta tgctcgtcaa
     4681 caaagcgagc gtttccctgc aatatttcac agtgattacg ttcgtaaaat ccctgacgca
     4741 tgcgcgtttg ttgattaatc acgttatcgg catggttaag gatatcggca aaagaagagc
     4801 ggagcagtcg ggaatggtcg ctgtaaagtg ggttttgatt gaattctata atgcggctga
     4861 cggcgtgacg gagagctttc gacgggatgg tgccccagtg ggtgcaaccg ccgccaacat
     4921 tttgataacg ctcgataact gcgacgcgcg caccttgctt aaccaggccc attgcagcgc
     4981 cttcgccgcc ggggccggaa cctattacta tggcatcgta atcgtaggaa tgtggcatgg
     5041 tagggcttac ctgttcttat acataaaagc aacagaatgg taacatttta tcgcgggtaa
     5101 gccaattgat ccccgtcatt tatctggcta tatcctgagc ggcctttgct ttgtctgttt
     5161 cttacttttg ccctgacgtt ttattggatt tttatcgacg atactctccg tttaagcggc
     5221 aggtttccgc tgtacgtaaa agaaccggcc aaagaattgc agtaaatatg ttttattgcg
     5281 ttaccgttca ttcacaatac tggagcaatc cagtatgttc attctctggt atagtgccag
     5341 cagtactttt ggcaaggatt cagacatcgt gatgggcgta agagcgcaac aaaaagaaaa
     5401 aacccgccgt tcgctggtgg aagccgcatt tagccaatta agtgctgaac gcagcttcgc
     5461 cagcctgagt ttgcgtgaag tggcgcgtga agcgggcatt gctcccacct ctttttatcg
     5521 gcatttccgc gacgtagacg aactgggtct gaccatggtt gatgagagcg gtttaatgct
     5581 acgccaactc atgcgccagg cgcgtcagcg tatcgccaaa ggcgggagtg tgatccgcac
     5641 ctcggtctcc acatttatgg agttcatcgg taataatcct aacgccttcc ggttattatt
     5701 gcgggaacgc tccggcacct ccgctgcgtt tcgtgccgcc gttgcgcgtg aaattcagca
     5761 cttcattgcg gaacttgcgg actatctgga actcgaaaac catatgccgc gtgcgtttac
     5821 tgaagcgcaa gccgaagcaa tggtgacaat tgtcttcagt gcgggtgccg aggcgttgga
     5881 catcggcgtc gaacaacgtc ggcaattaga agagcgactg gtactgcaac tgcgaatgat
     5941 ttcgaaaggg gcttattact ggtatcgccg tgaacaagag aaaaccgcaa ttattccggg
     6001 aaatgtgaag gacgagtaat gaaacaagca aatcaagata gaggtacgct gctgctggcg
     6061 ttagttgctg gcttatcgat taatggtact ttcgcagcgc tgtttagctc cattgtgcca
     6121 ttttctgtat tcccgattat ttccctggtg ctgacggttt actgcctgca tcaacgttat
     6181 cttaatcgca ctatgccggt aggcttgccg ggtctggcag ctgcctgttt tattctcggc
     6241 gtactgctgt acagcacggt agttcgtgcg gaatatccgg atatcggctc taacttcttc
     6301 ccggcagtac tctccgtcat tatggtgttc tggattggcg cgaagatgcg taaccgtaag
     6361 caggaagttg ctgagtaatc ggaatcatgc cggatgctgg cgcatccggc atgggtttta
     6421 cttcgcggtc agtaatacgc cgcactccat atggtgcgtg taggggaact gatcaaacag
     6481 agccagacgt tcgaccttgt gcgtctggct taatgtttcc agattcttgc ataacgtttc
     6541 cggattgcag gagatataca gaatacgtgg atacgcctgc accattttct cggtttcact
     6601 gtccagaccg ctgcgcggcg ggtcgacaaa aatggtttcg cactgataac tcttcaagtc
     6661 gatcccttgc aggcgattaa actcgcgtac accattcatc gcctgagtaa attcttctgc
     6721 cgccatacga ataatttgta cgttatcaat atggttagct gcgatgttgt attgcgcagc
     6781 agcaaccgac ggcttagcga tttcggtggc taatacccga tcaaaattgc gcgccagtgc
     6841 taatgaaaag ttaccgttgc cgcagtacag ctccagtaaa tcgccttttg agcctttggt
     6901 tacgtccagc gcccattcca gcatctgaat attcatcgcc gcgttcggct gggtaaaact
     6961 gttttctacc tgacggtaga tcatctcttt ccctgcgacc ggcagacgtt catcgatgta
     7021 atcttgatcc agctcgattt tggttttcgt tgcccgacca atcagatgca cattcagatt
     7081 ctgcgcgcgc agtgcatcgc gcagggcttc cgcttcctga cgccactcat catccagctt
     7141 tttatggtac aacagggaaa ccaccgcctg attactcagc gtggtgaggt aatcaatctg
     7201 gaacaacttg tggcgcagaa cgggattatt acgcacaccc gcaatcatcg ccgtcatcaa
     7261 ctggttgatg agttcactgg cagcggggaa gctatccacg cggatgcggc ttttggtttg
     7321 ttgatcgaaa atgatgtgat acaggtcatc gccatcgtgc cagatgcgga actccgcacg
     7381 catccggtaa tgactgaccg gcgagcgaaa cacttccgga accaggtcag aaaacggtgc
     7441 catcatactt tgcaaacgta ccactttttc ggctaactgc gcttcatact gttctgttgg
     7501 aaggtgttcg ggggtcatga tgtatcctga aaaattaaag tacgcggcga ttgtagggat
     7561 tgctcatcag atgtccagat cttgatgaat tcctatttgt gagctacgtc tggacagtaa
     7621 cttgttacaa cctgtagcat ccacttgccg gtcctgtgag ttaataggga atccagtgcg
     7681 aatctggagc tgacgcgcag cggtaaggaa aggtgcgatg attgcgttat gcggacactg
     7741 ccatccggtg ggaagtcatc atctcttagt atcttagata cccctccaag cccgaagacc
     7801 tgccggccaa cgtcgcatct ggttctcatc atcgcgtaat attgatgaaa cctgcggcat
     7861 ccttcttcta ttgtggatgc tttacaatga ttaaaaaagc ttcgctgctg acggcgtgtt
     7921 ctgtcacagc cttttccgct tgggcacagg ataccagccc ggatactctc gtcgttactg
     7981 ctaaccgttt tgaacagccg cgcagcactg tgcttgcacc aaccaccgtt gtgacccgtc
     8041 aggatatcga ccgctggcag tcgacctcgg tcaatgatgt gctgcgccgt cttccgggcg
     8101 tcgatatcac ccaaaacggc ggttcaggtc agctctcatc tatttttatt cgcggtacaa
     8161 atgccagtca tgtgttggtg ttaattgatg gcgtacgcct gaatctggcg ggggtgagtg
     8221 gttctgccga ccttagccag ttccctattg cgcttgtcca gcgtgttgaa tatatccgtg
     8281 ggccgcgctc cgctgtttat ggttccgatg caataggcgg ggtggtgaat atcatcacga
     8341 cgcgcgatga acccggaacg gaaatttcag cagggtgggg aagcaatagt tatcagaact
     8401 atgatgtctc tacgcagcaa caactggggg ataagacacg ggtaacgctg ttgggcgatt
     8461 atgcccatac tcatggttat gatgttgttg cctatggtaa taccggaacg caagcgcaga
     8521 cagataacga tggtttttta agtaaaacgc tttatggcgc gctggagcat aactttactg
     8581 atgcctggag cggctttgtg cgcggctatg gctatgataa ccgtaccaat tatgacgcgt
     8641 attattctcc cggttcaccg ttgctcgata cccgtaaact ctatagccaa agttgggacg
     8701 ccgggctgcg ctataacggc gaactgatta aatcacaact cattaccagc tatagccata
     8761 gcaaagatta caactacgat ccccattttg gtcgttatga ttcgtcggcg acgctcgatg
     8821 agatgaagca atacaccgtc cagtgggcaa acaatgtcat cgttggtcac ggtagtattg
     8881 gtgcgggtgt cgactggcag aaacagacta cgacgccggg tacaggttat gttgagaatg
     8941 gatatgatca acgtaatacc ggcatctatc tgaccgggct gcaacaagtc ggcgatttta
     9001 cctttgaagg cgcagcacgc agtgacgata actcacagtt tggtcgtcat ggaacctggc
     9061 aaaccagcgc cggttgggaa ttcatcgaag gttatcgctt cattgcttcc tacgggacat
     9121 cttataaggc accaaatctg gggcaactgt atggcttcta cggaaatccg aatctggacc
     9181 cggagaaaag caaacagtgg gaaggcgcgt ttgaaggctt aaccgctggg gtgaactggc
     9241 gtatttccgg atatcgtaac gatgtcagtg acttgatcga ttatgatgat cacaccctga
     9301 aatattacaa cgaagggaaa gcgcggatta agggcgtcga ggcgaccgcc aattttgata
     9361 ccggaccact gacgcatact gtgagttatg attatgtcga tgcgcgcaat gcgattaccg
     9421 acacgccgtt gttacgccgt gctaaacagc aggtgaaata ccagctcgac tggcagttgt
     9481 atgacttcga ctggggtatt acttatcagt atttaggcac tcgctatgat aaggattact
     9541 catcttatcc ttatcaaacc gttaaaatgg gcggtgtgag cttgtgggat cttgcggttg
     9601 cgtatccggt cacctctcac ctgacagttc gtggtaaaat agccaacctg ttcgacaaag
     9661 attatgagac agtctatggc taccaaactg caggacggga atacaccttg tctggcagct
     9721 acaccttctg aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg
     9781 gtctatgacg agatccggca tctcttaccg gatctccatt acatttatgc tttcgataac
     9841 gtcgcttttc cgtatggcga aaaaagcgaa gagtttattg ttgagcgagt ggtggcaatt
     9901 gtcaccgcag tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg caacaccgcc
     9961 agtaccgttt cacttcctgc attacgcgaa aagttcgact tcccggttgt tggtgtcgtg
    10021 ccggcgatta aacctgctgc acgtctgacg gcaaatggca ttgtcggatt actggcaacc
    10081 cgcggaactg ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc taatgaatgc
    10141 cagatagaaa tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa gctgcatggc
    10201 gaagatgttt ctctggatgc actaaaacgt atcctacgcc cgtggttaag aatgaaagag
    10261 ccgccagata ccgttgtatt gggttgcacc catttccctc tactacaaga agaactctta
    10321 caggtgctgc cagagggaac ccggctggtg gattctggcg cagcgattgc tcgcagaacg
    10381 gcctggctgt tagaacatga agctccggat gcaaaatctg ccgatgcgaa tattgccttt
    10441 tgtatggcaa tgacgccaga agctgaacaa ttattgcccg ttttacagcg ttacggcttc
    10501 gaaacgctcg aaaaactggc agttttaggc tgatttggtt gaatgttgcg cggtcagaaa
    10561 attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac
    10621 acggaacaac ggcaaacacg ccgccgggtc agcggggttc tcctgagaac tccg
//
LOCUS       DAEVZA010000153        10643 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEVZA010000153 DAEVZA010000000
VERSION     DAEVZA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.153"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..671
                     /gene="tus"
                     /locus_tag="LAF92_004261"
     CDS             <1..671
                     /gene="tus"
                     /locus_tag="LAF92_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBI9793179.1"
                     /translation="SKAAVRLPGVLCYQVDNLSQAALVSHIQHINKLKTTFEHIVTVE
                     SELPTAARFEWVHRHLPGLITLNAYRTLTVLHDPATLRFGWANKHIIKNLHRDEVLAQ
                     LEKSLKSPRSVAPWTREEWQRKLEREYQDIAALPQNAKLKIKRPVKVQPIARVWYKGD
                     QKQVQHACPTPLIALINRDNGAGVPDVGELLNYDADNVQHRYKPQAQPLRLIIPRLHL
                     YVAD"
     gene            complement(668..2071)
                     /gene="fumC"
                     /locus_tag="LAF92_004262"
     CDS             complement(668..2071)
                     /gene="fumC"
                     /locus_tag="LAF92_004262"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBI9793180.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(2214..3860)
                     /gene="fumB"
                     /locus_tag="LAF92_004263"
     CDS             complement(2214..3860)
                     /gene="fumB"
                     /locus_tag="LAF92_004263"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBI9793181.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            4059..5234
                     /gene="manA"
                     /locus_tag="LAF92_004264"
     CDS             4059..5234
                     /gene="manA"
                     /locus_tag="LAF92_004264"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBI9793182.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            5335..6843
                     /locus_tag="LAF92_004265"
     CDS             5335..6843
                     /locus_tag="LAF92_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBI9793183.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(6888..8153)
                     /gene="uidC"
                     /locus_tag="LAF92_004266"
     CDS             complement(6888..8153)
                     /gene="uidC"
                     /locus_tag="LAF92_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBI9793184.1"
                     /translation="MRKIVAMTVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGMGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(8192..9565)
                     /gene="uidB"
                     /locus_tag="LAF92_004267"
     CDS             complement(8192..9565)
                     /gene="uidB"
                     /locus_tag="LAF92_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBI9793185.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(9562..>10643)
                     /gene="uidA"
                     /locus_tag="LAF92_004268"
     CDS             complement(9562..>10643)
                     /gene="uidA"
                     /locus_tag="LAF92_004268"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBI9793186.1"
                     /translation="GEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPF
                     YFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGI
                     VVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPS
                     VVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDV
                     LCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDM
                     WSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPK
                     SAAFLLQKRWTGMNFGEKPQQGGKQ"
BASE COUNT         2647 a         2814 c         2560 g         2622 t
ORIGIN      
        1 gcagcaaggc cgctgtccgt ctgcctggcg tgttgtgtta ccaggtcgat aacctttcgc
       61 aagcagcgtt ggtcagtcat attcagcaca tcaataaact caagaccacg ttcgagcata
      121 tcgtcacggt tgaatcagaa ctccccaccg cggcacgttt tgaatgggtg catcgtcatt
      181 tgccggggct gatcaccctt aatgcttacc gcacgctcac cgttctgcac gaccccgcca
      241 ctttacgctt tggttgggct aataaacata tcattaagaa tttgcatcgt gatgaagtcc
      301 tggcacagct ggaaaaaagc ctgaaatcac cacgcagtgt cgcaccgtgg acgcgcgagg
      361 agtggcaaag aaaactggag cgagagtatc aggatatcgc tgccctgcca cagaacgcga
      421 agttaaaaat caaacgtccg gtgaaggtgc agccgattgc ccgcgtctgg tacaaaggag
      481 atcaaaaaca agtccaacac gcctgcccta caccactgat tgcactgatt aatcgggata
      541 atggcgcggg cgtgccggac gttggtgagt tgttaaatta cgatgccgac aatgtgcagc
      601 accgttataa accacaggcg cagccgcttc gtttgatcat tccacggctg cacctgtatg
      661 ttgcagatta acgcccggct ttcatactgc cgaccatctg ttctggccgt acccagctgt
      721 caaactcggc ttcgctaaga taccccagcg caagggccgc agcttttaag gtcagccctt
      781 ctttatgcgc ttttttggcg atctccgcgg ctttgtcata accaatgtgg gtgttaagcg
      841 cagtcaccag catcagcgat tcattgagta attgattgat tcgctcacga ttcggttcaa
      901 tacccactgc gcagtgtttg ttaaaacttt ccatgccatc tgccagcaag cgcaccgatt
      961 gcaggaaatt gtggatcacc attggacgga agacgttcag ttcaaagtta ccggaagcgc
     1021 cccccatgtt gatcgccacg tcgttcccca tcacctgaca gcagagcatg gttaatgctt
     1081 cacactgggt tgggttcact ttccccggca tgattgagct gcccggctca ttttccggga
     1141 ttgagatttc accaattccg cagcgcgggc cagaggccag ccagcggaca tcattggcga
     1201 ttttcatcag tgacgcagcc aaccctttca acgcgccgtg cgcctgaacc agggcatcac
     1261 aggtcgccag cgcttcaaat ttgttcggcg cggtaacaaa cggtgcacag gtaatgactg
     1321 ccagttcatc tgctacgcga cgcgcatact ccggatgggt atttagtcca gtacccaccg
     1381 ctgtaccgcc aagagccagt tccgctacgt gaggcaggct gtattcgata tgtttgagat
     1441 tatgctcgag catcgctacc cagccggaaa tctcctgccc cagtgttagc ggcgtggcgt
     1501 cctgcaagtg ggttcgaccg atttttacga tatcggcaaa tgcacgcgat ttttcactca
     1561 gcgtctgtgt cagggtttta agctgcggaa tgagttgctt gcgcagcgcc agcagcgccg
     1621 caacgtgcat cgccgtcgga aagacatcgt tggaactttg gcttttgttg acgtcgtcgt
     1681 tagggtgaac tttacgttcc atcccgcgca cgccgccgag taattcactg gcccggttag
     1741 ccagcacttc gttcatgttc atgttacttt gcgtgccgga gccggtctgc cagatagcca
     1801 gcgggaattc gtcgtcatgc tgtcctgcca gtacttcatc cgccgcctgc cgaatggcgc
     1861 tcgctttctc ttcagacaac aagcctaaat cttcattaac ttttgccgct gcgcgcttgg
     1921 ttagcgccag cgcatgaatc agtgaggtgg gcattttctc cgtcgaaatg cggaaatgct
     1981 ccagcgagcg ttgagtttgt gcgccccaca gcttatctgc cgggacatca atcgccccca
     2041 tcgaatcttt ttcgctgcgt actgtattca tggcctgctc ctcacctgtt taattttttc
     2101 tttctgtttt gctttcgtta agcaactttt agctcactta ttatttacta tttgataaca
     2161 aatgtttggt ctttcgtgcc atgtaaaaaa accgccccga agggcggctc tgtttatttc
     2221 acacagcgag tgcactgtgt gagttgtatc tgctggaaga agtcatttcc tttatcatcc
     2281 acaaggataa acgccgggaa atcttccact tcaattttcc agatggcttc cattcccagt
     2341 tccggatatt caacacattc caggctctta atacttccct gcgccaatac agcggccgga
     2401 ccaccgatac tgccaaggta gaagccgccg tgttttttac aggcatccgt cacctgctgg
     2461 ctgcggttgc ctttcgccag catgatcata cttccgccct gcgcttgcag ttgatcgaca
     2521 taagaatcca tccgtccggc ggtcgttggg ccaagagaac cggaggcata accttccggc
     2581 gttttggccg gacccgcgta gtaaatcgga tgatctttaa tgtactgcgg cagcccttca
     2641 ccgttatcca tccgctcttt cagtttggcg tgagcaatat cacgaccgac gataatcgtg
     2701 ccgttaagcg ataagcgtgt agaaacggga tactgcgaca actgtgcgag gatctctttc
     2761 atcggacggt taaggtcaac gcgcaccgct tcgccttctc ccgctttgcg cagctcttcc
     2821 gggatatatt tgcctgggtt atgttccagt ttttcgatcc agatcccctg acggttgatc
     2881 ttcgctttga tattacggtc agcagagcag gagacgccca taccgaccgg gcaggatgcg
     2941 ccgtgacgtg gcaggcgaat cacgcggatg tcgtgagcga agtatttacc accaaactgc
     3001 gcacccagac caagattttg cgcttcgatc agcaattctt tttccagttc cacatcgcgg
     3061 aacgcctgac cgtgctcatt cccttccgtt ggcagttcat catagtattt agcggaagcc
     3121 agtttgaccg ttttaaggtt cgtttctgca gaagttccac caataacgaa cgcaatatga
     3181 tacggaggac aggccgccgt acccagcgtg cgcatcttct caaccaggta atttttcagt
     3241 ttccccggcg ttagtaacgc tttggtttcc tgatagagat acgtcttgtt agccgaaccg
     3301 ccacctttgg cgatacagag gaatttgtac tcgtcgccat caacggcata aagatcgatc
     3361 tgcgctggta gattggtgcc ggtattcacc tctttataca tatccagcgg cgcgttttgc
     3421 gagtagcgca aattatcttc gatataagtg ttatagacac cgcgtgccag cgccgcttca
     3481 tcaccgccac cggtccatac acgctgccct tttttaccaa caataatcgc ggtgccggta
     3541 tcctgacagg ttggcagaac gcctttcgcc gcgatgtcgg agttgcgcag gaattgcagc
     3601 gccacatatt tatcattttc gctggcttcc gggtcacgca gaatgtcggc cacctgttgt
     3661 tggtgagccg gacgcagcat aaatgacgca tcatgaaatg cctgacgcgc caacagagtt
     3721 aacgcttcgg gtgcgacttt caaaatctcc tgcccttcaa attcagatac gctaacgtgt
     3781 tcgctggtta gcaggtaata ctcagtatca tcttttttga gtggaaaagg agcctgataa
     3841 tgaaagggtt tgtttgacat tgttctctca cttactgcct ggtttggtta tgctctgggc
     3901 gggtgttccg ttgccctgtt aaaagcgagt aacaatatcc tacacacttt tttaacaaaa
     3961 actgagacta gtacgacttt ttgcggctcc aggttacttc ccgtaggatt cttgctttaa
     4021 tagcgggatt aatttccaca ttaaaacagg gattgatcat gcaaaaactc attaactcag
     4081 tgcaaaacta tgcctggggc agcaaaacgg cgttgactga actttatggt atggaaaatc
     4141 cgtccagcca gccgatggcc gagctgtgga tgggcgcaca tccgaaaagc agttcacggg
     4201 tgcagaatgc cgccggagat atcgtttcac tgcgtgatgt gattgagagt aataaatcga
     4261 ctctgctcgg agaggccgtt gccaaacgct ttggcgaact gcctttcctg ttcaaagtat
     4321 tatgcgcagc acagccgctc tccattcagg ttcatccaaa caaacgcaat tctgaaatcg
     4381 gttttgccaa agaaaatgcc gcaggtatcc cgatggatgc cgccgagcgt aactataaag
     4441 atcctaacca caagccggag ctggtttttg cgctgacgcc tttccttgcg atgaacgcgt
     4501 ttcgtgagtt ttccgagatt gtctccctac tccagccagt cgcaggtgca catccggcaa
     4561 ttgctcactt tttacaacag cctaatgccg aacgtttaag cgaactgttc gccagcctgt
     4621 tgaatatgca gggtgaagaa aaatcccgcg cgctggcgat tttaaaatcg gcgctcgaca
     4681 gtcagcaggg tgaaccgtgg caaacgattc gtttaatttc tgaattttac ccggaagaca
     4741 gcggcctgtt ctccccgctg ttactgaatg tggtgaaatt gaatcctggc gaagcgatgt
     4801 tcctgttcgc tgaaacaccg cacgcttacc tgcaaggcgt ggcgctggaa gtgatggcga
     4861 actccgataa cgtgctgcgt gcgggtctga cgcctaagta cattgatatt ccggaactgg
     4921 ttgccaatgt gaaattcgaa gccaaaccgg ctaaccagtt gttgacccag ccggtgaaac
     4981 aaggtgcaga actggacttc ccgattccag tggatgattt tgccttctcg ctgcatgacc
     5041 ttagtgataa agaaaccacc attagccagc agagtgccgc cattttgttc tgcgtcgaag
     5101 gcgatgcaac gttgtggaaa ggttctcagc agttacagct taaaccgggt gaatcagcgt
     5161 ttattgccgc caacgaatca ccggtgactg tcaaaggcca cggccgttta gcgcgtgttt
     5221 acaacaagct gtaagagctt actgaaaaaa ttaacatctc ttgctaagct tattaaaggc
     5281 ttataacacc ttcaggcggc cagtccgcct gatttcattt tatggataat cattatgaat
     5341 aaatcgctgg tagcggtagg cgtcattgtt gcgctaggcg tagtctggac aggcggcgca
     5401 tggtatacag gcaagaagat tgaaacccat ctcgaagaca tggtcgcgca ggcgaacgcg
     5461 caactcaaac tgacagctcc tgaatccaac ctggaagtga gttatcaaaa ctatcatcgc
     5521 ggcgtattca gcagccagtt gcaactgttg gtgaaaccca ttgccgggaa agaaaatccg
     5581 tggattaaaa gcggtcagag cgtcatcttc aacgaatcgg ttgatcatgg tcccttcccg
     5641 cttgcccagc ttaaaaaact gaacctgatc ccgtcgatgg catcaattca aaccacgctg
     5701 gttaataacg aagtaagcaa accactgttt gatatggcaa aaggtgaaac gccttttgag
     5761 attaactcgc gcattggtta cagcggtgat tccagttccg atatttcgct caagccactg
     5821 aattacgagc aaaaggatga aaaagtcgcc tttagcggcg gcgagttcca gttaaatgcg
     5881 gacagagacg gcaaagctat ctccctttcc ggggaggcgc aaagtggtcg gatagacgca
     5941 gttaacgaat acaaccagaa agtgcagttg acctttaata atctgaaaac cgacggttcc
     6001 agcacgctgg caagttttgg tgagcgcgta ggaaaccaaa aactgtcact ggaaaaaatg
     6061 accatttcgg tggaaggcaa agaactggca ctgctggaag gcatggagat cagcggtaaa
     6121 tcggatctgg tcaatgatgg taaaacgatc aatagccaac tggattactc gctaaacagc
     6181 ctgaaggtac agaatcagga tctgggcagc ggcaagctga ctttaaaagt cggccagatt
     6241 gatggtgaag cctggcatca gtttagccag caatataacg cgcaaactca ggcgctgctg
     6301 gcacagccag aaattgccaa caatcccgaa ctttatcagg agaaagtgac ggaagccttc
     6361 tttagcgccc tgccgctgat gttgaaaggc gatccggtga ttactatcgc gccgctaagc
     6421 tggaaaaaca gtcagggtga aagtgcgctg aatctgtcgc tgttcctgaa agatccggca
     6481 acgactaaag aagcgccgca aacgctggcg caggaagtgg accgctcggt taaatctctg
     6541 gatgcgaaac tgaccattcc ggtggatatg gcaactgagt ttatgactca ggtagcgaag
     6601 ctggaaggtt atcaggaaga tcaagcgaaa aaactggcga aacagcaagt tgaaggtgca
     6661 tcagcaatgg ggcagatgtt ccgtctgacc accttgcagg acaataccat caccaccagc
     6721 ctgcaatatg ctaacggtca gataacgtta aacgggcaga aaatgccact ggaagatttc
     6781 gttggtatgt ttgcaatgcc ggcattaaat gttccggtcg tacccgctat tccgcagcag
     6841 taattcacag acaaaacgcc ggagattttt ctctccggcg ttatttttta cttcagcata
     6901 aagtcatact gaatactcat tttcacatcc cagtaattca ggcgatcgcc cagcgtactg
     6961 ccatcgcgct tttcccgccc tttatcggca gcaagaaaga aattcatccc ttgtaacggc
     7021 ccttcgctga cccgatagtt cataccacct aagataccca tgccttcaat ccgcacatct
     7081 cgtttactgg attcatagcc gcgagtcaca atcggtgcaa atgtcagtgt aaattgcggc
     7141 gttaaacgat agttgacgcc caattgccag gactgcattc cttcacggtt acgatcaaga
     7201 cttaatgaaa agggatagcc aatgtcggta tccaccacgg caccattcat tgcatggcgc
     7261 aaataaccac cactgccaaa tacgcttccc cacggcgcat tccaggtcaa ttgtccacta
     7321 accaacgccg tttcattggg ctggctggta ttacgactta aaccttcgag ctgcgcgtaa
     7381 aagccgagca actccccttt caacgtataa ttcgggtcga gcggtatgct tttctcgaga
     7441 atactttgta tctgattgga aacatctttc tgtaccgatg cccccagaga atagcgactg
     7501 gtatcatcat gccaactggc ggctaaaaaa gaacgtgcgc gatgtttatc gtattttccc
     7561 gtctggctgt tatacacccc ttcatcacga ataccggttg ttgaatcagt ccataaaaat
     7621 actcgtgaac gccacattcc ccacagatca atatttggtg ttaaagccca ataacctaat
     7681 gctccttcag aaactgtcgg taatgtacgt tgcgaactgc cagccattaa cggaatacga
     7741 taaggtaagc taccataact ataatccgtc ccgaagcgac ctaatttaag caggctattt
     7801 tcactgggtt ttatttttac tgcccccagg gcaaagccaa aacttttatc accatcaaga
     7861 taccaccggg tactcatatc agcacgagca cctaatttat aaacataata cgccccgcct
     7921 tcaacgccaa taatattgga ataataacca ctattgaaat cgagcaatcc tccctgtacc
     7981 catgcgtaaa tatctcttcc cgcgccagca ctgggcttat ccgccctgcg caattcgttt
     8041 ttcagtctga tgcgtagtcc cgcttcatcg tcagccagtt gcgccgcata agcagaggta
     8101 aggccagagg cagccgtcag gcaaataacg gtcatggcca ctatttttct catagttgca
     8161 cctttgatgt tctgattatt tttattgaat attaattagt gatatcgctg attaattgct
     8221 gctgcacttt tttacgatta tcaatttcaa ccacgatttc tttgaatttt ttatccgtga
     8281 gcggataaaa ccagataata acgaatgcca gtagcataaa tccgcaaggt actaaggcaa
     8341 ttgatgtgcg gatgcccata ataacttccg gcgtttgcac ctgattggcg atatatccgc
     8401 ttaatcccaa aataaaggca ggaattgaac ctccgattgc ctgaccgcat ttacgggtaa
     8461 atgagaatag tgaataggtg agcccttcaa ttcgcacgcc ggtcaggtat tcaccgtatt
     8521 ctacggtatc agcttccagt gcccacatca cagtcatagt aacgccctga ccaattgaag
     8581 cgatggccaa cgcaaccaac gccaccggca gtgaccagac ggaaacccag aagaatagca
     8641 gataaccgca ggttcccagc aaagcgccaa tcaggaaggt attcttttta ccgatcctcg
     8701 cgaccatccc cggcaccagc ggtgccgatg ccacagtacc aaccaggttt tgcaccagta
     8761 ccagcacagt gaacagcccg gtatcattta acacatagcg cacgtagaac aacgacgagg
     8821 cgctgaccgc gaaggtcgaa atcagcacac acagcgcacc gatgcacaac ataaacagcg
     8881 ggcgattccg tttcagggtt tgcagactga tcttcaatga cggctgcgcc acgatacgta
     8941 ccacattctc acgcgtcgat ttgaagcaga tgaagtaaag caccattccg gcaatcgcca
     9001 gcacaatcgt ccagaaatgg tataccgaca ccatctcttc cgggctggag ttcttaatgc
     9061 tcggtcctat cagaaatgcc aggcagacaa aggttaatga agcggcaatc ccgcgagccg
     9121 cgcccagacg ggcgcgggat tgtggttgtt gggtcatcgc ggtagcaagt gaaccataag
     9181 gaatattcac caggctgtag caaagcccga ggcccatgta ggtcaaatat gcatacacta
     9241 ctttgctgct atggctccag tcggtcggta cccagaatac cagcacgctg aagatcatta
     9301 acggcgcagt accgaagagt aaaaacgggc ggaattttcc ccagcgggta ttcacactgt
     9361 ccaccactcg tccggcaaag acgtcggcga aggcatcgaa tacccgcacc agtaacagca
     9421 tggtgcccgc cgcagcggca ccgacgccag cgacgtcggt gtagtaactc aacaggaaga
     9481 gcgcccccat tgcgaaggcg aagttattgg cgacgtcacc gaggctgtag ccgacgatgg
     9541 tgcgccagga gagttgttga ttcattgttt gcctccctgc tgcggttttt caccgaagtt
     9601 catgccagtc cagcgttttt gcagcagaaa agccgccgac ttcggtttgc ggtcgcgagt
     9661 gaagatccct ttcttgttac ctccaacgcg caatatgcct tgcgaggtcg caaaatcggc
     9721 gaaattccat acctgttcac cgacgacggc gctgacgcga tcaaagacgc ggtgatacat
     9781 atccagccat gcacactgat actcttcact ccacatgtcg gtgtacattg agtgcagccc
     9841 ggctaacgta tccacgccgt attcggtgat gataatcggc tgatgcagtt tctcctgcca
     9901 ggccagaagt tctttttcca gtaccttctc tgccgtttcc aaatcgccgc tttggacata
     9961 ccaaccgtaa taacggttaa ggcacagcac atcaaagaga tcgctgatgg tatcggtgtg
    10021 agcgtcgcag aacattacat tgacgcaggt gatcggacgc gtcgggtcga gtttacgcgt
    10081 tgcttccgcc agtggcgcga aatattctcg tgcaccttgc ggacgggtat ccggttcgtt
    10141 ggcaatactc cacatcacca cgcttgggtg gtttttgtca cgcgctatca gctctttaat
    10201 cgcctgtaag tgcgcctgct gagtttcccc gttgactgcc tcttcgctgt acagttcttt
    10261 cggcttgttg cccgcttcga aaccaatgcc taaagagagg ttaaagccga cagcagcagt
    10321 ttcatcaatc accacgatgc catgttcatc tgcccagtcg agcatctctt cagcgtaagg
    10381 gtaatgcgag gtacggtagg agttggcccc aatccagtcc attaatgcgt ggtcgtgcac
    10441 catcagcacg ttatcgaatc ctttgccacg caagtccgca tcttcatgac gaccaaagcc
    10501 agtaaagtag aacggtttgt ggttaatcag gaactgttcg cccttcactg ccactgaccg
    10561 gatgccgacg cgaagcgggt agatatcaca ctctgtctgg cttttggctg tgacgcacag
    10621 ttcatagaga taaccttcac ccg
//
LOCUS       DAEVZA010000154        10567 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEVZA010000154 DAEVZA010000000
VERSION     DAEVZA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.154"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1293
                     /locus_tag="LAF92_004269"
     CDS             <1..1293
                     /locus_tag="LAF92_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBI9793187.1"
                     /translation="VSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETFLPELFQNH
                     GYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAG
                     TAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPND
                     NPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTIILFTSDNGA
                     VIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYPTALDAA
                     DISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRH
                     QSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQV
                     VKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1345..2502
                     /locus_tag="LAF92_004270"
     CDS             1345..2502
                     /locus_tag="LAF92_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBI9793188.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2468..2590)
                     /locus_tag="LAF92_004271"
     CDS             complement(2468..2590)
                     /locus_tag="LAF92_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793189.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2793..4478
                     /locus_tag="LAF92_004272"
     CDS             2793..4478
                     /locus_tag="LAF92_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBI9793190.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            4516..6888
                     /locus_tag="LAF92_004273"
     CDS             4516..6888
                     /locus_tag="LAF92_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBI9793191.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGNNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6945..9728
                     /locus_tag="LAF92_004274"
     CDS             6945..9728
                     /locus_tag="LAF92_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI9793192.1"
                     /translation="MRNFCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWGNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLVEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGDLSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWVSLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQEKQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRINIREQASG
                     AYSVSSRLSIDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVRQYLS
                     HPVNTYTGVLLPK"
     gene            10090..>10567
                     /locus_tag="LAF92_004275"
     CDS             10090..>10567
                     /locus_tag="LAF92_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI9793193.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTNKPNLVC"
BASE COUNT         3090 a         2344 c         2326 g         2807 t
ORIGIN      
        1 gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg ctttggtgtc
       61 tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt gcctgaatta
      121 ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc aaaaatcagt
      181 aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt caccacattt
      241 tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg attccacgct
      301 gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg tgtccccgca
      361 aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga tcgtgccaaa
      421 acacttgacc agccatttat gctttacctg gcttataatg ctccgcacct gccaaatgat
      481 aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac agcagataac
      541 tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga acaactgaag
      601 aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg tgcggttatc
      661 gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac ctatcctggc
      721 ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg taattatgac
      781 aagctgattt ccgcaatgga tttctacccg acagctcttg atgcagccga tatcagcatt
      841 ccaaaagacc ttaagctgga tggcgtttcc ttgctgccct ggttgcaaga taagaaacaa
      901 ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt tgacgaggaa
      961 aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga cgattacccg
     1021 cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa taacgattat
     1081 tcgcttgtct atacagtaga aaacaatcag ttaggtctct acaaactgac ggatctacag
     1141 caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca aggcgtggta
     1201 agagagttta tcgacagcag ccagccaccg cttagcgaag taaatcagga gaagtttaac
     1261 aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc ggaattttcc
     1321 gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag ttttcaatgt
     1381 aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac tcatgaaaaa
     1441 tggatggatg acagcactct gaaagagttc atcaaacaat atatcgcagc gtctggcaat
     1501 caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga ttttttccgt
     1561 aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa tgcattacaa
     1621 acgaatggca ttttattgaa taatgaatgg tgtgcttttc tcaaagaaca tgagtttctg
     1681 gttggtatct cgatcgatgg cccccaggag ctacatgacc gttacagacg cagtaattca
     1741 ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc atatcaagtt
     1801 gagtttaata cgttaaccgt cattaataac gttaatgtcc attaccctct tgaggtttat
     1861 cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct cgaaaccggg
     1921 acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat tgatttttct
     1981 gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg ggttaaaaac
     2041 gatgtgggtg aaattttcat tcgtcagttt gaaagctttg tcagccgttt tttggggaat
     2101 gggcatacaa gttgtatttt ccaggagtcc tgcaaggata atctggttgt tgaaagtaat
     2161 ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg aaacattaat
     2221 aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa acggatttca
     2281 gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc taagcatcgt
     2341 attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa aatccttttt
     2401 tcaaccatgg taccttatat gaacgccatg gtggagttag ctaagaacag agtaccgctt
     2461 taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc tcttttaact
     2521 ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt cctgtcgggt
     2581 gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg cgtcagggca
     2641 ttagcaatac ccttgattat caatagcata accataacca ctgaaaatga aatattcctt
     2701 tatatttcat attgttaaat gatacttaaa taaatttcat cagtaaatca ttgccataga
     2761 taatcattgg atggcacaat caattttcgc taatgataac cattcccatt acgttttgta
     2821 tgttgatagc aaagtatctc tgtctgctga agcccttttg gctgcgtaag aataataaaa
     2881 cttcagtgtt gttaatcatt attatcctcg ccatgatcct tggcgtggtt aaaattcagg
     2941 tctggttaaa cgactggaat aatgattttt tcaatgcgct aagccagaaa gaaaccgata
     3001 agctttggca gcttgtcctt tggttccctg ccctgctggg gatctttgta ttaatatccg
     3061 taaacaaaac atggttaatt aaactactga ctattcgctg gcgcgaatgg ttaacagatt
     3121 actaccttaa tcggtggttt gcagataaaa actactactt cacgcagatc tacggcgagc
     3181 ataaaaatac agataaccct gaccaacgta tcgctgaaga cattctctta ctgatcagca
     3241 aaacgctcag cctttcattt ggcttcatcc agtcacttag catgctgatc acctttaccg
     3301 ttattctttg gcaaagcgcg ggtacgctct cattcactgt tggtggaaca gaatggaata
     3361 tccaggggta tatggtctat accgttgtgc tcattgtgat cggtggaact ttatttaccc
     3421 ataaggttgg taaacgtatt cgtccgctta atgtggaaaa acaacgtagc gaagcgacat
     3481 ttcgaacaaa tcttgtgcag cataataagc aggcagaact gattgcgcta agcaatgctg
     3541 agtctctaca acgccaggag ttgagcgaca attttcatac cattaaagag aactggcatc
     3601 gtttaatgaa tcgtcagcgg tggcttgatt actggcagaa tatttattcg cgctcgctta
     3661 gcgttcttcc ctactttctg ttattgccgc agtttattag cgggcagatc aatctgggcg
     3721 gactgatgaa atcgcgccag gcatttatgc tggtatcgaa caatttaagc tggtttattt
     3781 ataaatatga cgaacttgct gagctggctg cggttatcga tcgcttgtat gagttccatc
     3841 aactcactga acagcgccct acgaataagc ctaaaaattg ccaacatgcg gtacaagtgg
     3901 ctaatgcgag tattcgtacg cctgataata agatcatatt agagaacttg aactttcatg
     3961 tttcgccagg caaatggcta ttactgaaag gctactctgg tgcgggaaaa accacactgc
     4021 ttaaaacatt atcccactgc tggccgtggt ttaaaggtga tatttcttct cctgctgaca
     4081 gttggtatgt gtcacaaaca ccgttaatca aaagcggctt actgaaagag attatttgta
     4141 aagcacttcc cctgcccgta gacgataaat cgttgagcga agtactgcat caggttggtc
     4201 tggggaaatt ggctgcgcgt attcatgacc acgatcgctg gggagatatt ctttccagcg
     4261 gcgaaaaaca acgtatcgcc ctggcacgat taattttacg acgtccgaaa tggatatttc
     4321 ttgacgaaac tacctctcat cttgaggaac aagaggctat ccgcttactg cgtttagtgc
     4381 gtgaaaaact acccacaagc ggcgtcatta tggttacaca tcaacccggt gtctggaacc
     4441 tggcagatga tatttgtgac attagcacgg ttttataaca acaataaaag ccctgctgta
     4501 cggagatata aataaatgaa gcgagttctt attcctggcg tcattttatg tggcgctgat
     4561 gtggcgcagg ccgtcgatga caaaaacatg tacatgcatg tttttgaaga gatgacggtc
     4621 tatgctcctg tccctgtacc cgtaaacggc aacacgcatt acaccagtga aagcatcgag
     4681 cgtttaccga ccgggaatgg caatatcagc gatctgctga gaaccaaccc tgcggtacgc
     4741 atggattcaa cgcaaagtac ctcgttgaac cagggagata ttcgcccgga gaaaatctct
     4801 attcacggtg cgtcgcccta tcagaatgcc tatttgattg acggtattag tgccactaat
     4861 aacttgaacc cagcgaatga gtccgatgcc agtagtgcaa ccaatattag cgggatgtca
     4921 caggggtatt atcttgatgt cagcttactg gacaatgtga cgctttatga cagttttgtg
     4981 ccggttgaat tcggtcgctt caatggcggg gtaattgatg caaagatcaa acgcttcaac
     5041 gctgatgata gcagcgtgaa actgggttat cgcactacgc gttcggactg gttaacatcg
     5101 catatcgatg agaataacaa gagcgcattt aatcaaggct cttcaggaag tacttattac
     5161 tccccagatt ttaaaaagaa cttttatacc ttgtcgttta atcaggaact cgctgataac
     5221 tttggcgtta ccgccggttt atcgcgccgc cagtctgata tcacccgcgc ggattatgtt
     5281 tcgaatgacg gcattgtcgc cggtcgggca cagtataaaa acgttatcga taccgcattg
     5341 agcaaattta cctggtttgc cagcgaccgc tttacccacg atttaacctt aaaatatacc
     5401 ggctccagcc gtgattataa taccagcacc ttcccgcagt ctgatcgcga aatgggtaat
     5461 aaatcctatg gtctggcatg ggatatggat acacagctcg catgggccaa actgcgtact
     5521 accgttggtt gggatcatat tagtgattat acccgtcacg atcatgacat ctggtacacc
     5581 gaactttcat gtacatatgg tgatattaca gggcgttgca cccgtggcgg attaggacac
     5641 atttcccagg ctgtagataa ttacaccttc aaaacacgcc tggactggca aaaattcgcc
     5701 gtgggtaatg tttcgcatca accctacttc ggcgcggaat acatctattc cgatgcatgg
     5761 actgaacgcc ataaccagtc tgaatcctat gtgattaatg ctgcaggaaa gaaaactaac
     5821 cataccattt accataaagg taaaggcagc ctgggaattg acaactacac gctgtatatg
     5881 gcggatcaca ttagctggcg gaatgtgtcg ttaatgcccg gtgtgcgtta tgactatgac
     5941 aactatctgt caaaccacaa tatctccccg cgctttatga cggaatggga tatttttgct
     6001 gatcaaacct caatgattac agcaggttat aaccgttact atggcgggaa tattcttgat
     6061 atgggattac gtgatatccg caatagctgg acggaatcgg tatcaggcaa taaaaccctg
     6121 acgcgttatc aggatttgaa aacgccttat aacgatgaac tggcaatggg attgcagcaa
     6181 aaaatcggta ataacgttat tgcgcgcgca aactatgttt accgtgaagc gcatgatcaa
     6241 atcagcaaaa gcagtcgtac cgacagcgcg actaaaacca ccattactga atataacaac
     6301 gatggcaaaa ccaaaacgca ttcgttcagc ctcagttttg aactggccga acccctgcat
     6361 atccgccagg tagatattaa cccacaaatt gtctttagct atatcaagag caagggcaac
     6421 ttgtcgttaa acaatggtta tgaggagagc aataccggtg ataaccaggt ggtttataac
     6481 ggtaatctgg tctcttacga tagcgttcca gtggcagatt ttaataaccc attaaagatc
     6541 tccttaaaca tggatttcac gcatcaaccg agcgggttgg tgtgggcgaa tacgctggcc
     6601 tggcaagaag cgcgtaaagc tcgcattatc cttggtaaga cgaatgcgca atacatcagc
     6661 gaatattcag attacaagca gtatgttgac gaaaaactgg atagcagcct gacctgggac
     6721 acccgcttgt cctggacgcc acaatttctg aaacaacaaa acctgacgat cagtgccgat
     6781 attctcaatg tactggatag caaaaccgct gttgatacaa cgaataccgg tgtggcgacc
     6841 tacgccagtg gccgtacttt ctggcttgat gtcagcatga aattttaata gttcggattt
     6901 tacttaaccg ggtaaattcg cccggttttc gcatggagat aattatgaga aacttctgtt
     6961 tcttactgac gttagtggca actctgttgc tccccgggcg gctgattgcc gccgccttac
     7021 cgcaggatga aaagttaatt accggacaac tggacaatgg cttgcgatat atgatttatc
     7081 cgcatgctca tccaaaggat caggtaaatt tatggctgca aattcatacc ggttcattgc
     7141 aggaagagga caatgagcgc ggcgtggctc attttgtaga acatatgatg tttaacggca
     7201 caaaaacatg gccgggtaat aaagttatcg aaacatttga gtcgatgggt ctgcgctttg
     7261 gtcgcgatgt taatgcctat accagctatg acgaaacggt gtatcaggtg agtttgccga
     7321 ctacgcagaa acaaaatctg caacaagtga tggcaatatt cagtgaatgg ggtaatgccg
     7381 caacctttga aaaactcgaa gtagacgctg aacgtggcgt aattactgag gaatggcgtg
     7441 cccatcagga tgcgaaatgg cgcacctctc aggcgcgccg ccctttcctg ctggctaata
     7501 cccgtaattt agaccgtgaa cctatcggcc tgatggatac tgtcgccacg gtcacaccgg
     7561 cacaattgcg ccaattttat caacgctggt atcaaccaaa taatatgacc tttatcgtgg
     7621 tcggcgatat cgacagtaaa gaagcgctgg cgctgattaa ggataattta agtaagcttc
     7681 cggctaacaa agcagctgaa aatcgcgtct ggccgacaaa agccgaaaat cacctgcgct
     7741 ttaatatcat taatgataaa gaaaaccggg tgaacggcat cgcactctat tatcgcctgc
     7801 caatggtaca agtgaacgat gagcaaagct ttatcgaaca agctgaatgg agcatgttag
     7861 ttcagctgtt caatcaacgg ctgcaggaac gtatacagtc gggcgagttg aagactattt
     7921 ctggtggcac tgcgcgcagc gttaaaattg cacccgatta tcagtcgctg tttttccgtg
     7981 taaatgcacg tgacgataat atgcaggatg ctgcgaatgc attaatggca gagttggcaa
     8041 ccattgatca gcatggtttt tctgctgaag aactcgatga tgtcaaatct acccgcctca
     8101 cctggctgaa aaatgcggtt gatcagcaag ctgaacgtga tttacgtatg ctgaccagtc
     8161 gcctggcatc cagctcatta aataatacgc cgttcttgtc gccggaagag acatatcaac
     8221 tttcgaaacg tctgtggcag caaattaccg tgcaaagtct ggtggaaaaa tggcagcagt
     8281 taagaaagaa ccaggacgca ttttgggagc aaatggtaaa caatgagctt gccgctaaaa
     8341 aagcattgtc tcctgcggct atcctggcgc tggaaaaaga gtacgccaac aaaaagctgg
     8401 cggcttacat cttcccaggc agaaatttat cgttaacagt agacgctgac ccacaggcgg
     8461 aaattagcag caaagaaacg ctggctgaga atctgacatc attaacactt tctaatggtg
     8521 ccaaggttat tctggcaaaa tccgcgggtg aagagcaaaa gctacaaatt actgccgtat
     8581 ctaataaagg cgatttaagt ttccctgcgc agcaaaaatc acttatcgcg ctggcaaata
     8641 aagcagtaag cggaagcggc gttggtgacc tctcctcttc cagcctgaaa cgctggagtg
     8701 cggaaaactc ggtaaccatg agcagtaaag tcagtggcat gaatacgttg ctctccgtta
     8761 gcgcgcggac taataaccct gaacctggtt tccagttgat taaccagcga atcacccaca
     8821 gcacgattaa cgacaatatt tgggtatcgc tacaaaatgc tcaaattcag gcgttgaaaa
     8881 cgctcgacca gcgtccagcg gagaaattcg cccagcagat gtatgagacg cgctatgctg
     8941 atgaccgcac gaaattactg caagaaaaac agattgcaca gtttactgcc gcagatgcgc
     9001 tggctgccga tcgccagttg ttttcatctc cagcggatat cacgtttgtc attgtcggta
     9061 atgtcgcaga agacaaactc gtggcgttaa ttacacgtta cttaggctca atcaaacact
     9121 ctgattcgcc attagccgca ggtaaaccat taactcgcgc gacggacaat gcatcggtta
     9181 cggtaaaaga acaaaatgaa cctgtggcac aggtttcaca gtggaagcgt tatgattccc
     9241 ggacaccggt gaatctggcg acgcgtatgg cgctcgatgc ttttaacgta gcactggcaa
     9301 aagatctacg cattaatatt cgtgaacagg catctggggc atacagcgtt tcttctcgcc
     9361 tctcgattga tcctcaggcc aaagatatca gtcatttgct ggcttttact tgtcaaccgg
     9421 aacgacatga tgaactgtta acgttagcga atgaagtgat ggttaagcgt ctggctaaag
     9481 ggatcagtga gcaagaactg aacgaatacc agcaaaacgt tcagcgcagc ctcgatatcc
     9541 aacagcgtag cgttcaacaa ttagcgaaca ctattgtaaa tagtcttatt caatatgacg
     9601 atcctgcagc atggactgag caggagcaat tgttgaaaca aatgacggta gagaatgtta
     9661 acactgccgt tagacaatat ctttctcatc cggtgaatac ctataccgga gtattattgc
     9721 caaaataata acagccccgt caacacctcg ttggcgggga ttttagcaat attcgccatt
     9781 tttatgtaat aattttataa atgcgttcaa aataatagcc gagtgctaat agtgatattt
     9841 taaggtctga tttttacgcg ataattcagg aggcatagaa agcgcataaa aataacagca
     9901 taaaacacct taccaccacc catgaatttc atattacatt gtttttcaat gaaaaaatat
     9961 tattctcgta atatctcacg ataaataaca ttaggatttt gttatttaaa cacgagtcct
    10021 ttgcacttgc ttactttatc gataaatcct acttttttaa tgcgatccaa tcattttaag
    10081 gagtttaaaa tggataagaa gcaagtaacg gatttaaggt cggaactact cgattcacgt
    10141 tttggtgcga agtctatttc cactatcgca gaatcaaaac gttttccgct gcacgaaatg
    10201 cgcgacgatg tcgcattcca gattatcaat gacgaattat atcttgatgg caacgctcgt
    10261 cagaacctgg ccactttctg ccagacctgg gacgacgaaa atgtccataa gttgatggat
    10321 ttatccatta acaaaaactg gatcgacaaa gaagaatatc cgcaatctgc agcaatcgac
    10381 ctgcgttgcg taaacatggt tgccgatctg tggcatgcgc ctgcgccgaa aaatggtcag
    10441 gccgttggca ccaacaccat tggttcttcc gaggcctgta tgctcggcgg gatggcgatg
    10501 aaatggcgtt ggcgcaagcg tatggaagcc gcaggtaagc cgaccaacaa accaaacctg
    10561 gtgtgcg
//
LOCUS       DAEVZA010000155        10426 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEVZA010000155 DAEVZA010000000
VERSION     DAEVZA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.155"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..214)
                     /locus_tag="LAF92_004276"
     CDS             complement(<1..214)
                     /locus_tag="LAF92_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBI9793194.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQLLDRVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(466..840)
                     /locus_tag="LAF92_004277"
     CDS             complement(466..840)
                     /locus_tag="LAF92_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HBI9793195.1"
                     /translation="MLTFWVGFVAGCTPLHPSDCHKTTATGSCSSGRWDDQDEWGAQA
                     RGIRAAINAKLDEPHNWKGKKCRLHMEFSQDGTALKISTSNGDKAYCEAIKSAAHKAK
                     FPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(1012..1440)
                     /gene="tolA"
                     /locus_tag="LAF92_004278"
     CDS             complement(1012..1440)
                     /gene="tolA"
                     /locus_tag="LAF92_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBI9793196.1"
                     /translation="MGFRSASILTLIISGIIIGCTDAVSTNYHDRTSYYSDKAIETQY
                     VSSSERTSDVSEDIRLYAHQIKSAIEKQFGDASKYSGKECTLRMHMAPNGLLLEVKRE
                     SGDLDLCREAMNAIKNADIPAPPSPEVYKVFQNGVLDFKP"
     gene            complement(2486..3028)
                     /locus_tag="LAF92_004279"
     CDS             complement(2486..3028)
                     /locus_tag="LAF92_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBI9793197.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLASKKLTKTAINEALRRMKKAGIEKAELEAFLREMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLICILRQRYEGRGMAKRKMAELLNDAHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAEKMA"
     gene            complement(3025..3315)
                     /locus_tag="LAF92_004280"
     CDS             complement(3025..3315)
                     /locus_tag="LAF92_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBI9793198.1"
                     /translation="MVNLRKAAKGQMCQIRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKKGHL"
     gene            complement(3315..3914)
                     /locus_tag="LAF92_004281"
     CDS             complement(3315..3914)
                     /locus_tag="LAF92_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBI9793199.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDEV
                     EFQQVYKSVLNVLWNWILFRKFSSQEEVENVAAQLLEFA"
     gene            complement(4378..4476)
                     /locus_tag="LAF92_004282"
     CDS             complement(4378..4476)
                     /locus_tag="LAF92_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI9793200.1"
                     /translation="MTVQVARKDLCEVRIRTDQTEVAVFTAYELGE"
     gene            complement(4603..6672)
                     /locus_tag="LAF92_004283"
     CDS             complement(4603..6672)
                     /locus_tag="LAF92_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI9793201.1"
                     /translation="MKGNILGDIRAEHDEKMLEASFWQTTDYKALLESYDRCIIVGRR
                     GTGKSALVHMLSKHWHAKPKTYVMTISPVEEQIIGLRDVISLFGENYLHIKAGSKLAW
                     RYAIYMELLSEIASHYKMKNDLDYKSVESHLLSWGPKRQNISSKIRKKLNAILDDNKN
                     IKPATRIAELSDKFELDLLEEVIEEAISKSNNQFVIFADRLDEGYTPDNLGVAIVDGF
                     IQAVIDIKQNMNEKVIAFAFVRDNIHRAISKMDPDFTRNIEGQVLRLHWDEYNLFNLV
                     CNRMRVAFNSTIENNTRVWNAYTANELQSNTGFKETLKLTLYRPRDILVLLNNAFLRA
                     NTHDRNRIVIDDIQATANTISQNRLNDLLKEYENVFPALDIFTSQFANSKPYYSVSEA
                     SSKIQYALDLNGVIDKMKMQDLLLFEGPIQVIQRLYSVGFIGLYNQQSSSYVFCHDGK
                     EPEKEFTPDSKLLLHPCYWLALSVHESEMTPDAADDIHDEYDIEVSSVSEEQRKQRIG
                     SLLQELHNIPEGPEGAVDFEAWALKTIKLLFATNLTNIELHPNKNGLQQRDIIATNIA
                     DTPVWKRILTDYQTRQVVFEVKNYKTLGAAEYRQVNSYLFKDYGRIAFIINRDHTENL
                     EKHKELTWVKEIYDNHNKLVVKLPSKFLERHLSKMRSPQKHDEVNKQLGKLLDQYIRV
                     YLNNKCK"
     gene            complement(6926..7675)
                     /locus_tag="LAF92_004284"
     CDS             complement(6926..7675)
                     /locus_tag="LAF92_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001471407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HBI9793202.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREAVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPMQAELPEETTTATVVKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAEPKAASVTETDFKAFPQ
                     PAGVALPVQKDATQEDIKTETVADIVQSLPSFTATREDDLILPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHWDIVHKVIAAGMEK"
     gene            complement(7698..8444)
                     /locus_tag="LAF92_004285"
     CDS             complement(7698..8444)
                     /locus_tag="LAF92_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI9793203.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            complement(8451..9239)
                     /locus_tag="LAF92_004286"
     CDS             complement(8451..9239)
                     /locus_tag="LAF92_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793204.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVETNGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTKTDQKALPEEEKEEDINTYLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            complement(9317..9739)
                     /locus_tag="LAF92_004287"
     CDS             complement(9317..9739)
                     /locus_tag="LAF92_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793205.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQDALSTAIDAHDDAVQAVYRKAHFSGGGSPSDSVVVH"
     gene            complement(9736..9990)
                     /locus_tag="LAF92_004288"
     CDS             complement(9736..9990)
                     /locus_tag="LAF92_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBI9793206.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            10070..>10426
                     /locus_tag="LAF92_004289"
     CDS             10070..>10426
                     /locus_tag="LAF92_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI9793207.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQIRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEK"
BASE COUNT         2818 a         2482 c         2041 g         3085 t
ORIGIN      
        1 attcacctct cgcagccttg cgcttatctt ctttaatctt gaaataaagg tttgtcaggt
       61 acgtcagtag gccaaatacc agactaccca gcacaccgat tgcagcccac tgtgacggag
      121 ttactctatc gagcaactgt aaaaaccagt agccagcact gcctgcggag gtgccgtagg
      181 caatgcctgt tgaaattttg tccatggatt tcatagcctc acctccgcac ggaacggatg
      241 gcatagttat tatgtgtagg ctttcagaca catcaatcag agccttaatt gatatatatg
      301 ctggagacga tgcaatataa aaagctcgcc gtagcgagct aataaaatgt atttctctga
      361 tattatgttt atttgtatta gctcagactt gacatcacag gtttcgtata tagaacatca
      421 tcaaatctgt cagtttgcta tgaatgagat atagtaattg aagagctaac ctcgcatgtc
      481 aaagccagat ttctgaaaat ctctgtagac ttccggattg ttgaaggccg gaaatttggc
      541 tttatgagct gcggacttta tcgcttcgca ataggcttta tcaccgttac tggtagatat
      601 ttttaacgcc gtgccatcct gagagaattc catatgcaac ctgcattttt tccctttcca
      661 gttatgcggc tcatcaagtt tggcattaat tgcagctctg attccccgcg cttgcgcccc
      721 ccattcatcc tgatcatccc agcgtcctga actgcaacta cctgtagcag tagttttgtg
      781 gcaatctgaa gggtgtaaag gtgtgcatcc cgcaacaaaa ccgacccaaa aagtcaacat
      841 aacgattttc tttaatccca cttcttgctc ctcaatccat taaaatctca gcaatagtag
      901 ttgttacgtc cgccactggc tcagagctga ctatccgcta aatttagctc agtgccgtag
      961 ctgtgtcaga acaaacctaa gccgaaaccg tttattacaa aacaataaat atcagggttt
     1021 aaaatccagc accccatttt gaaatacttt atatacttcc ggcgaagggg gggcaggtat
     1081 atcagcattc tttatcgcat tcatcgcttc acgacataaa tcgaggtctc cactttctct
     1141 tttaacctcc agtagaaggc cattcggggc catatgcatt ctcagtgtac actcttttcc
     1201 tgaatactta ctcgcatccc cgaactgttt ttcgatggcg ctcttgattt gatgggcata
     1261 cagacggata tcctcactaa catcagaagt acgttcagat gaactcacat actgtgtctc
     1321 tattgcttta tcggagtaat atgatgtacg gtcatgataa tttgtcgata cagcatcagt
     1381 gcacccgata ataatcccac taataatcaa cgtaagaatt gatgcgctac gaaaacccat
     1441 ttttcctcac atatgtcata tagtaaagga ttatatatac cgttgttttg gacgctcaaa
     1501 cagcgaatca gatcaaataa aacgcacatt tgttaacatt tacacaaagt ctgcgtggga
     1561 tattctgaaa gaatatccat aatgtggaga gaatctattg aagtgcatgg tgccgggtgc
     1621 ctcccggtga acaaaatgtt cgtgatacct gtcggcgaca gaaaaggtta atggtatcac
     1681 cccaccgcac agggggattc accatgcagg agttttctta gcaaactcac tgcgcgcccg
     1741 gcaactccca accacataaa atgcggagtt tgtggtattt atgcatataa ctcgcaggaa
     1801 ttatcttaaa aaactgatgt cgatccggat taaaaagaag caggtcatca tcagatgact
     1861 ggaaaaaagg aaaacaaaaa atactcatca tacagttttg attgcaggga tgagcctgct
     1921 atgcacaata tgcagaatat aagcaagata aaaatatgca ggcatattat ttcggatttt
     1981 gttattaaca caaccttttt aataatcatt tggcatacaa taaaccagcc caaaaagaac
     2041 cgcctaaaca ggcggttggt taatacaaag gatgcttcgt ctttattata gtaatctgag
     2101 gcgtcgggtg tcttgtatca gacaacatat tgtcccgcta aacagcgaat tacaaaccac
     2161 cctgcaatga tctctcatct cattttatat gagttgacga catcaggata acgcatcatc
     2221 agcccctgcc aagaaatatc aaaactcccg ccagcaatgt gttatcacaa tattgtaaaa
     2281 aaaacacagc accgaaacta taactggtct ctgttataat ttggagcaga aagaccagtt
     2341 gcccaactag cagcattctc ccctgctttc ctgacgtaaa aaaaaccgca ttaagcggtt
     2401 tttttacgat gtccatgtct gcaatccgcc tcgcgataca gctttgcgaa gcatagcaaa
     2461 attgaagcag tttatacgta agaaatcaag ccattttctc agcaaatgat tcacgcatgg
     2521 gaatatatag ggcatactca gcaacagcta accaattagc aatccgtttt tcgcatgtgc
     2581 taaaacacca ctctgggtgt gcatcattta gcaattcagc cattttgcgc ttagccatcc
     2641 cccgcccttc atagcgttgc cggagaatgc aaatcaatcc tggatgctct gccagcacct
     2701 cacttatgac tcgatcaata cataacgcct ctgcatcagt acaatgcgcc agccagctct
     2761 tttgcttgcc gttgatcatc tctcgcaaaa acgcttccag ctcagctttc tctattcccg
     2821 cttttttcat tctgcgcagg gcttcattaa tggctgtttt cgtcagtttt ttggatgcca
     2881 acaactgatt gaacatattc cctgacctgc caccgccaat atacgaccag cgcccccaca
     2941 tacgtagttt tccctgaatc cagacacttt ccagcgtggt gaggcgaagg tgttctccgc
     3001 tttttcctgt attcgttggg taaatcataa atgacctttc tttctccaga tttcttgtgt
     3061 gcgaaaaacc ccttcagcat gcatcaggcg caattcttct ttggtgtaat cgctggtttt
     3121 tacccgcccg tcgattagat cgtggcatga gctacaggct atcgccgcct gcatatcgtg
     3181 tggttttgtc gctgttccgc acgtccccgc cagcctgtaa tgcgccagca cagaggtttc
     3241 gggattgtga ttgcagtagc cagggattct gatctggcac atctggcctt tagccgcttt
     3301 acgtaaattc accattacgc aaactccagt agttgtgcgg ccacattttc aacttcctcc
     3361 tgagaggaga atttacggaa cagaatccag ttccacagca cattcagtac agatttataa
     3421 acctgctgaa actcgacttc gtccatattc gcaaaagcga tggattttgc ccgacgccca
     3481 cggctaccgt ccggataaat atgctcggtg taaaatccgg cctgaatggt tacccactcg
     3541 cggaaagcct caaacgactt tagcaacgcc gtatcccggg ttctacgagt cgcaacggtg
     3601 ttaaggtatt gctctgcggc atcactcagg gctggcgtgt gttcccgacc aactgattcg
     3661 cacaggtaat caacgaaacc agacagcagt tctcgttcgc gaggcgtgat cgccccaccg
     3721 accggagtcc agtaatcgaa tcccagttgc aggagtttga aaaaacgctt gtggaatgcg
     3781 tagttacgca cacgcttaaa gtctgcgtgt atccactcac ctattttgat ttgatgcaga
     3841 aaatcgcaac tctccggcgt cgccgggaga agtaaaccag aagaagtttg tttgaccagt
     3901 tgtatatgcg ccatttctca atctctcgat ggcgcagtgc agcagatgcc agttgttcag
     3961 gctgacgtat aaagtataaa taaactggtt ccagtgtaaa gcccccacct taatggaata
     4021 aagaccaaac aacagattgc tgggatacaa acaacgctta ttattaaaag cggttaaaca
     4081 aattaaattt taatgttatg caaatttgtc agatcaccat aatatctcat ttgaaaaccg
     4141 ctgaaataac agccctatca gggttaatca tattaaggtg agtaaatatg gaaaacaaca
     4201 aatctgtaca ttacgttcct tttttatctg taatactttt tgttttatgc tgtgcgtggg
     4261 cattattttt ataaaaatat ttacagatga agtaaacccg ccaatcaggt taactgtggc
     4321 tgcgttgagg atgcataata catcagaggt tgcggggatt tctccccgct ggtcctctta
     4381 ctccccaagt tcgtaagctg tgaagacagc gacctccgtc tggtcggttc ggattcgtac
     4441 ctcgcagagg tctttccttg caacctgcac cgtcataata acggtaaatg ttgaatcgtt
     4501 cgtaaattaa tctatacaga ttattatata gctctcatta cgattttgat tactctcact
     4561 taaaaatacc gaaagataca tgatctttcg gtaaagattg aattacttac atttattatt
     4621 taaatagacc cttatgtact gatcgagcag tttacctaat tgtttattaa cttcgtcatg
     4681 tttttgcggg cttctcattt ttgacagatg cctttcaagg aacttagagg gtaacttcac
     4741 aactaattta ttatgattat cataaatttc ttttacccac gtaagctctt tatgtttttc
     4801 taaattctct gtgtgatctc tatttatgat aaaagctatc cgcccatagt ctttaaacaa
     4861 ataggaattt acctgtctat attctgcagc acctaatgtt ttatagtttt tcacttcaaa
     4921 aacaacttgc cgtgtctgat aatctgtcag aattctcttc caaactggag tgtcagctat
     4981 atttgtcgct ataatatctc tttgctgcaa cccattttta ttgggatgca actcaatgtt
     5041 cgttaaattc gtagcaaata gtaactttat agttttgagc gcccatgctt caaaatcgac
     5101 agcaccttca ggtccttcag gtatattatg tagttcttgg agcaacgaac cgattcgttg
     5161 tttcctctgc tcttcggaaa cagagctgac ctcaatatca tattcatcat gaatatcatc
     5221 agcagcatcg ggagtcatct ctgattcatg aacacttaat gctagccagt agcatggatg
     5281 caatagcaat tttgaatctg gtgtaaattc tttttcaggt tccttaccgt catggcaaaa
     5341 tacatatgaa gatgactgct ggttatacaa cccaataaat ccaacactgt aaagtcgttg
     5401 aatcacctgg attggccctt caaaaagaag taaatcctgc attttcatct tgtcaataac
     5461 gccattgaga tcaagggcat attgtatctt agatgacgct tcagaaacac tgtagtaagg
     5521 tttgctatta gcaaattgcg atgtaaatat atctaatgca ggaaatacat tctcatattc
     5581 ttttaacaaa tcattgagtc tattttgtga aatagtattc gctgttgctt gaatgtcatc
     5641 aatgacaata cgattacgat catgtgtgtt tgctcgcaag aatgcattat ttaacaaaac
     5701 caaaatatct cttggcctat atagagtcag tttcaatgtt tctttaaaac cggtattaga
     5761 ctgtaattca tttgcggtgt aagcattcca caccctcgtg ttgttctcaa tggttgagtt
     5821 aaaagcgacc ctcattctat tgcatacaag attaaacaag ttatattcat cccaatgtaa
     5881 tctcagtact tgcccttcaa tatttcgagt aaaatcaggg tccatttttg atatggctcg
     5941 atgaatatta tctctaacaa atgcaaaggc aataaccttt tcattcatat tttgttttat
     6001 atcaatcaca gcctgaatga aaccatcaac tattgcaaca ccaagattat ccggtgtata
     6061 gccttcatca agcctatcag caaaaataac aaattgatta tttgacttcg aaattgcttc
     6121 ttctatcacc tcttccaata agtcaagttc aaatttatcg gataattcgg ctattcttgt
     6181 agctggtttt atatttttgt tatcatcgag aatagcgttc aatttttttc ttattttgct
     6241 acttatattt tgtctcttcg gtccccaaga taataaatgc gactctacac ttttgtagtc
     6301 aagatcgttt ttcatcttgt aatgacttgc gatctcagaa agcagctcca tataaatagc
     6361 atatcgccat gctaacttgc taccggcttt tatatgtaag taattttcac caaataaaga
     6421 aataacatcc cttaatccaa taatttgttc ttcaaccgga ctaatagtca tcacataggt
     6481 ttttggctta gcatgccaat gtttggaaag catatgcacc agagcacttt ttcctgttcc
     6541 tctgcggcca acaatgatgc atcgatcgta ggattcgaga agagctttat aatcagttgt
     6601 ttgccaaaat gaagcttcca acatcttttc gtcatgttca gctcggatat ccccaagaat
     6661 attacctttc atccagattc ctttttaacc cattgaggtg acagcacaaa tagtatctta
     6721 acacctgatg atttcatcag aacctgataa atagacgtga tataaatatg actaagatca
     6781 catttcatga aaaaattcga cgagtttata ttaactgcag cgaatacaaa tcaccgcagt
     6841 tttcattacc cctatctgaa gaactctgca caaccgatca tattggtcac aactgcagca
     6901 ggatgactca tataatcagg agtatttatt tctccatacc tgctgcaata actttatgga
     6961 caatatccca gtgcttgttc agctcccgca gcgcggcgca gactcgctcc cacttctgga
     7021 catgattctt cgccctacgc agttcgcggt ttgccatatg cagcgatggc aaaatcaaat
     7081 catcttctcg cgttgcagta aacgatggca gcgactgcac aatgtccgct acagtttctg
     7141 ttttaatatc ttcctgtgtt gcatctttct gtaccggtaa cgcaacacct gctggctgag
     7201 gaaaggcttt aaaatcagtt tccgttaccg atgcagcttt cggctctgct ggtaaattat
     7261 cgcccggtat gcagtaacga aatttaccgt tctggtttac gcgaatcagg cgtcctttgc
     7321 tgattgccat tgccagcgtt gaagcaactt tgcgggatgt tgtaccgaac agcgtagcca
     7381 gttcatccgc cgtttgtggt ccgcgttgtt caatcgtcgc ggttaaatcg cactctgaga
     7441 ttttcactac agttgccgtg gtggtttctt ccggcagttc tgcctgcatt ggctgttcct
     7501 gctgaacgtt gttatcagcc acacgccagg tgtacgcgct tttatcaacg aaaccagctt
     7561 ttttcagttc ccatagttcg ttcagcactg cttcacgact gatatcaagt cgcgcagcaa
     7621 gttctatgga tgtggctttt cccattgctt tcagtgcgtc aaaaacagtc tccattaaat
     7681 ttttctcccg gtaaaaatta cttcgcaatt cctggctgga cgacattcgg acgccagctc
     7741 tcccagttga aattcaccca tcgcccgccg ttcatggtca tgcgatccat aatccgctcg
     7801 ccaagcaatg ttttcatggc ctcatagttc aggtttgtca gcatccccac gctgcgcatc
     7861 gacgctgtcc ggcgatcaac aatctggtgc agtaccacct gctcgttttt cgtctcgcgc
     7921 tgaatgccaa tttcatcaag aaccagcaga tccacttcgc acagttcccg caaaaatttt
     7981 tcgcctgact gcccgtcgtc atagctggcg tgcagggcac tcataacatc agccacggta
     8041 accacaatca ctgtctgacc gtctttcagc aggcgattcc cgatagctgc cgctaagtgg
     8101 ttcttcccgg taccaggttt tccgctgaac gcaaaatttg tacacccggt catcagttca
     8161 tcagcgatgg atttcgcctg actcaacgcg tatcgctgcc cttcgttctg cacctggtaa
     8221 ttcgaaaacg agcatttgcg gtgcaatggc tggatgccag agcgattcag aattttttcc
     8281 acccgcaact gacgattctg acggttgatc tcctcacaac gtttctggcc ttcggaaagt
     8341 tgccactcgc gccactccgc taccgtcttg aatggcgcgg ttacatgtga cggggccagt
     8401 ctgcggatac gttcaagaac gtcgcctgtc gcaatatttt tcatggtcag ttaccccctg
     8461 aagcctggcg ggatcgcact atccggtaac gagacggtgt taacctgtcg gagtaacgtc
     8521 tcaggtcgaa cacctttcgg cgcgaacaag ccctggtatt cgttggcgat gctgtgtcga
     8581 atcacctgct caggtgaaaa accctgctgg cggaattttt ccagctcccg tatcgccccg
     8641 ttagcgccct gctccgttcg aatcggtttt cgcaatgcct gcctgaactg aacccactca
     8701 tgccagagtg tttccggcaa ccagtcaggc agctcgatag cctccggttc gaatttttta
     8761 gacgctcgtt tttggcgagg gggatttagg gggagataag tatttatatc ttcctctttc
     8821 tcttcctctg gtaacgcttt ttgatccgtt tttgtaacgc tggcagcgtt accttttcgt
     8881 ttcagttctc gtatttttgt tactctctcg tttgtaaccg cccgtttttt agagcttttc
     8941 ccgttatggc gctcaaagtt aggaagcgac aacacaccat tagtttcgac cagccatcca
     9001 acctgaatta acgcatcagc aaaaccagcc ataaaagtga tgcgatctat tgcacttttt
     9061 gtaacgccgc gagcgttaca ctctgcgtta ccgtctatca tttgttgatc cgcccatgcc
     9121 cagaagcgaa tgactttccc taatgcggca tctggatcaa tattcagaat ctcagcaagc
     9181 ctgaatattt ccggcttatc cggcgtaata acttcgagct ttatccagtt tgaagccatt
     9241 tgttttcacc ttgtaacgct cgcagcgtta catttaactg ataccgaaca aaacagttcg
     9301 gtacgattaa tttcaatcaa tgcactacga cagaatcgct aggagaaccg ccgccgctga
     9361 aatgtgcttt acggtaaacg gcctggactg catcatcatg cgcatcaatt gccgtactca
     9421 gtgcatcctg tgccgccagt aatgcacggc gttccagggt atcgaagatg cagagtcggt
     9481 gacgcagctc gcgaggaaga attgccagaa ccgcagggat cagtttctga attttttccc
     9541 tttgcgcatt cgtttcacct ttcaaccaac gatgatagat attctgctga ttgttccagt
     9601 ccttgcctgg taccaggggc aattcgccgc ccccctggcg cagatattct tcagtaattg
     9661 cgttagcgac ccacgcctgc cctttttcgg ctgccagggc taacaacact gattcgatgt
     9721 gctcatgctt gattttcatg aatcaactcc catcagcttt ttcgtagtag ttttatttct
     9781 gccaatagtt aaaattgcat cggcagaaaa taatccgttt gatgcatgag cgattttttc
     9841 agcgtaattt gtttcgccgg tatattctgt gcgaggcaat tttccgttat ccatccattt
     9901 gtagattgct ctttggctga caccacaaac gtcggccaca acagaaacgc gaacagtttt
     9961 gattacatct tcaagtgttt tctggttcat atcaccctca caatgtgaac tttgagtaca
    10021 tgctataaca gaactgacag tacattcaag agcgaatatc attgaactta tggttcatga
    10081 agataaagcg cgtaaagagt tcgccagtag gcttgcgcta gcctgtgaaa acgctggtta
    10141 tgaacaacat ggaaggcagg cagaaattgc ccgtcgaatg aaattaacac caaaagcggt
    10201 tagcaaatgg tttaatggtg aaacaattcc tcgccgagag aaattaaggg aattagcaac
    10261 actcattgga acaacaccaa cctatctttt gggagaggat acagaagaaa gtggacagat
    10321 acgtttctat caggagttaa atccaagaca aaaaatcatc attgaccttc tggacgagct
    10381 ccctgacagt gagacagatg aacttttaaa aactcttgag gagaaa
//
LOCUS       DAEVZA010000156        10393 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEVZA010000156 DAEVZA010000000
VERSION     DAEVZA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.156"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..771
                     /locus_tag="LAF92_004290"
     CDS             <1..771
                     /locus_tag="LAF92_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBI9793208.1"
                     /translation="KYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIES
                     RKREIEGPFGEFTGHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPAT
                     CVPLYQQLKAEFPEVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVK
                     MVIMVDEDVDPFNLPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIID
                     ATTPVAPDNRGHYSQPVVDLPETKAWAEKLTAMLAARK"
     gene            782..1018
                     /locus_tag="LAF92_004291"
     CDS             782..1018
                     /locus_tag="LAF92_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793209.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(1057..2421)
                     /locus_tag="LAF92_004292"
     CDS             complement(1057..2421)
                     /locus_tag="LAF92_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBI9793210.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(2510..3286)
                     /locus_tag="LAF92_004293"
     CDS             complement(2510..3286)
                     /locus_tag="LAF92_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBI9793211.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(3291..3929)
                     /locus_tag="LAF92_004294"
     CDS             complement(3291..3929)
                     /locus_tag="LAF92_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBI9793212.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(3926..5188)
                     /locus_tag="LAF92_004295"
     CDS             complement(3926..5188)
                     /locus_tag="LAF92_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HBI9793213.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(5185..6093)
                     /locus_tag="LAF92_004296"
     CDS             complement(5185..6093)
                     /locus_tag="LAF92_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBI9793214.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            6289..7056
                     /locus_tag="LAF92_004297"
     CDS             6289..7056
                     /locus_tag="LAF92_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI9793215.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(7107..7763)
                     /gene="pphB"
                     /locus_tag="LAF92_004298"
     CDS             complement(7107..7763)
                     /gene="pphB"
                     /locus_tag="LAF92_004298"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBI9793216.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(7869..>10393)
                     /gene="mutS"
                     /locus_tag="LAF92_004299"
     CDS             complement(7869..>10393)
                     /gene="mutS"
                     /locus_tag="LAF92_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBI9793217.1"
                     /translation="MQQYLRLKAQHPEILLFYRMGDFYELFYDDAKRASQLLDISLTK
                     RGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATSKGPVERKVVRIVT
                     PGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEPADRETMAAELQRT
                     NPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFGTRDLVGFGVENAP
                     RGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRNLEITQNLAGGAEN
                     TLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDFTAELQPVLRQVGD
                     LERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQALREKMGEFAELRD
                     LLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERLEVRERERTGLDTL
                     KVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKEYEDKVLTSKGKAL
                     ALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLNYTCPTFIDKPGIR
                     ITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYMRQTALIALMAYIG
                     SYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILHNATEYSLVLMDEI
                     GRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKMEGVANVHLDALEHG
                     DTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELESISPNAAATQVDGT
                     QMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
BASE COUNT         2487 a         2793 c         2711 g         2402 t
ORIGIN      
        1 aaatatgatc agtccgagta cgaaatggca ggcgcgctgc gtgaaagccc gtacccgatc
       61 gccaccgccc cgttgaccgg ttttgatgtg ccgtggggtt cagaagtgat cctcgaaggg
      121 gtcatcgaaa gccgtaaacg cgaaatcgaa gggccgttcg gtgagtttac cgggcactac
      181 tccggcgggc gtaacatgac cgtggtgcgc atcgataaag tctcttaccg caccaggccg
      241 attttcgaat cgctgtacct cggtatgccg tggaccgaaa tcgactacct gatggggcca
      301 gccacctgcg tgccgctgta tcagcagctg aaagccgagt tccctgaagt gcaggcggta
      361 aacgccatgt acacccatgg cctgctggcg attatctcca ccaaaaaacg ctacggcggc
      421 tttgcccgcg cggtgggcct gcgcgcaatg accacgccgc atggtctggg ctacgtgaag
      481 atggtgatta tggtcgatga agacgttgac ccgttcaacc tgccgcaggt gatgtgggcg
      541 ctctcctcga aagtgaaccc ggcaggggat ttggtgcagt tgccgaatat gtccgtgctg
      601 gaactcgatc caggctcaag ccctgcgggg atcaccgaca agctgattat cgacgccact
      661 acgcctgtcg ccccggacaa ccgtggtcac tacagccaac cggtggtgga tttaccggaa
      721 accaaagcct gggctgaaaa actgaccgct atgctggctg cacgtaaata aggagaagaa
      781 gatgatttgt ccacgttgtg ccgatgaaca gattgaagtg atggcgaaat cgccggtgaa
      841 agatgtctgg acggtatatc agtgccagca ttgcctttat acctggcgcg ataccgaacc
      901 gctgcgccgt accagccgcg aacattatcc cgaagcgttc cgcatgacgc agaaagatat
      961 tgatgacgcg ccaatggtgc cgagcatccc gccgctgctg gtggaaggta agcgttaaat
     1021 aaaaggtccg atgcccatcg gaccttttta ttaaggtcaa attaccgccc atacgcacca
     1081 ggtaattaag aatccggtaa aaccgagaat ggtcgttaac actgtccagg ttttcagacc
     1141 gtctgctacc gacaacccca gatatttggt cacaatccag aaccctgagt cattaatatg
     1201 tgacgcgcca agcccaccaa agcaggctgc cagcgtcacc aatacgcact gaatcggatt
     1261 caatcccatc accgcttctg agagtaaccc gccggttgtc aggattgcta cggttgctga
     1321 cccctgcgat gcacgcagag ccagtgaaat aataaatgcg gctggtaaca gaggcaggtc
     1381 aatcatttgt agcatattgg caagggcttt gccgacgccc gattccacca gcactttgcc
     1441 aaatacccct ccagcaccag taaccaaaat cactaccgcc gcagtgggaa gggctgagcc
     1501 cataatgtcg ctggtgtgtt gtaagctcca gccgcgacgt aaagccaata accagaatgc
     1561 cagcaccagc gcaatcatta gagctaccat tggtgagccg atcagctgta gcgtaccaag
     1621 caggggatgc gaaggcggca tcagtgttgc ggaaaccgta cccgccatga taatcgcgat
     1681 aggaataaca attagcgagg tgaccagcgc gacgcccggt ggatttattt tatcgcttaa
     1741 ttttgttgcg ccttcctcac tggccggagc cagttgcatc tgttccagta cttccaccga
     1801 catcgcatat tggcgcgtat tgattatttt cgctgcaaag tagccaacaa tccctacggg
     1861 aatagaaatc gcaataccga tgatggttag ccagccgatg tctgcgtgga gtaaccccgc
     1921 tgcggcgaca gggcctggat gcggcggtac cgccacatga acagttagca tgattccagc
     1981 gacaggcagg ccaaatttga gtggcgatat tttggcaacc ttggcaaaac cgtaaatgat
     2041 tggcgcaaga ataataaagc cgacatcaaa gaagacggga ataccgagga agaacgctgc
     2101 cagagtcagc gcagcgatag ttcgtttgtc acctaacttg cgactgaaat aattagccag
     2161 tgactctgca ccaccagagt gttcgatcat acgccccagc atagcgccca gaccaataat
     2221 aatagtgacg gaaccaagca caccgcccat cccggcgatc atcactttac ccacttcgcc
     2281 cgccggtata cccgccgcaa gtgcgactaa caggctgacg aggagcagag caacgaatgg
     2341 ttgtaccttt gccttgatga ccagcagcaa cagcatgatt acgccagcta acgcaatgca
     2401 taacaatgta attgtggaca tgggaaaccc tgtctgaaag ttatagttaa cctaccccat
     2461 ccgtagatgg ggggatgtat gggtacgttg taattaggga tttaacgaat tagcgccagg
     2521 cgtcaaacca gccaagccct tcttcggtga ggccacgagg tttatattca caaccgatcc
     2581 agccctgata tcccacctca tcgaacaggc ggaacagcca cggatagttg atttctccat
     2641 cgtccggttc atgtcgatca ggtagtccgg caatttgtac gtgcgcatat ttcccagcgt
     2701 agtcgcggat taaatgcgtc aggttgccat ctactttttg cgcatgaaaa gtatctagtt
     2761 gaataaacac gttatctcgc gcaacctctt caacaatagc cagtgcctga tactggctgg
     2821 agaagagata atgaggctta acgtcggggc tgagtgcttc aactaatatt cgcttgccgt
     2881 gtagcgcaaa gcggtcggca gcgtagcgga gattatcgat aaatactgcc cggtaccgtt
     2941 cagcgtcttc gccagcgggc acgacgcctg ccatcacatg gacttgttca caattgagcg
     3001 ccaatgcata ttccagtgcc aggtcgatgt ctgcgtgtgc ttcgtgctca cgtccgggaa
     3061 gggcggataa tccccattcc cccgcattaa tatctccggg agcggtattg aacagcgcca
     3121 gtgtcagatg gttttgctcc agttgctttt ggatttgcag ggtggagtag tcatagggaa
     3181 acagaaattc cacagcatcg aacccggctt ttcgcgctgc ggcgaagcgt tcaataaaag
     3241 gcacttcggt gaacatcatg gataaattag ctgcaaaacg aggcattgca ttaactcctt
     3301 aattccgcaa tttcacctgc ggtcagataa cggatcgggc ggtcaccgag aataaaaatc
     3361 agctttgccg tttcctccag ctcttccata ttgttggcgg cttcttgcag gctttcaccg
     3421 caaaccactg ggccatgatt tgccagtaaa aaagcctgat tgtctgctgc cagttccgcc
     3481 agatcctgtg cgatgcgttt atcgcccggt cggtaataag gcaccagcgg gacatttccc
     3541 atccgcatca ccacgtatgg tgtgaacgga cgaataacgt tgctgctgtc cagcccttgc
     3601 aggcaggaaa gcgccgtcga ccatgtgctg tgcaaatgca ccaccgcttt acagcgcgga
     3661 ttgttgcgat acagcgccag atgaaagagc acctctttcg agggtttgtc accacttaac
     3721 cattcgccat ccgcggcgac tttggaaagc cgctgcggat cgagattgcc caggcatgaa
     3781 cctgtcggtg tcgccagtaa attcccgtca ggtaaaagca gcgacagatt gccagccgaa
     3841 ccggttgcat agccgcgctg aaagaatgaa ctggcaatcc gcgtcatctc ctctcgcaaa
     3901 gactgctcta cttttgcgaa atcgctcatg ataaaaactc tctttgggct cgtgaaaaaa
     3961 aggcttcatc accgaagttg ccagatttaa gggcgagtga gacaggctta tccagtgcgt
     4021 ttacccacgg cacgccgggg gaaatggttg ggccaatatg aaaccctttt atgcccaggc
     4081 tctgtgtgac tacgccggag gtctcaccgc ctgcgacaat aaagcgtgtc acgccttccg
     4141 ctgctaaccg cgccgctagt tgagaaaata gagtttctac tgcctgactg gctttttgtg
     4201 caccgtattg ctgttgaatt gctgccaatg cgtcagtgct ggcggtggca aaaaccagtg
     4261 gagcaagtac actttcctgg cccagaaccc actctgccag ttcgtgtgca taagcggcca
     4321 gagtttcggt tgagaggcag cgtgccacat caacttcacg ggctggtgca atttgacggt
     4381 aatgtgccac ctggcggttg gtcatttgag agcatgaacc ggagagcact acgccgcgcc
     4441 cagcgagcgg atgccctgct tcgcgagcct ggttaccgtt ttcttgcgcc cactgccggg
     4501 ccaggccaat cgccagacca gaaccgcccg ttaccagtgg ggcatcgcgc aaggcttctc
     4561 cctgaatttc cagatggtgt tcggtcagcg catcaagcac cgcgtagcgg tagccctctt
     4621 gctgtaagcg agccagctct tgacgaacgg catccacacc ttgttcgaaa acatgtgccg
     4681 aaacgacgcc gcagcgccct gtggattgcg cttcaaccag acggggaaga tagctgtcgg
     4741 tcatgggatt taccgggtga tggcgcatcc cggattcggc cagcagttga ttcattacga
     4801 acaaataccc ctgataaacc gtacgtccgt tgaccggcag ggccggagag aagaccgtaa
     4861 acggcgtgtc gagagcatcc attaatgcat cggtaaccgg gccgatatta cctttcgccg
     4921 tactgtcgaa agtagagcag tatttgaaat agatctgttt gcaaccttgc tgttgcaacc
     4981 agctcagagc cgccagcgat tgctgtgtgg cttcaaccac cggacaggag cgcgttttca
     5041 ggctgatcac cagtgcgtcg attgcttccg gcattttacc tgttggaaca ccgttaattt
     5101 gtaccgttgg tagaccgttt tccaccagaa aactggcgat atccgtcgcg ccggtaaaat
     5161 catcggcgat aacgccaatc ttgatcatga tttcgctccc ggtagagtga tgccagagaa
     5221 aatcttgata actgcgctat cgtcttcttt cccgtaacct gcgttactgg cgctggtgaa
     5281 catattcaat gctgttgagg ccaatggcag cgggaagtgc agggctttgg ctgtatcggc
     5341 aaccagacca agatccttaa caaaaatatc gacggctgaa tgcggggtgt aatcgccatc
     5401 caccacatga cgcatccggt tttcgaacat ccaggaattt ccggcggcat tggtcacgac
     5461 gtcatacatc acatccagcg ggatccccgc acgggctgca agtgccatcg cttcggctcc
     5521 ggcagcaata tgtacgcccg ctaacaactg gtgaataatt tttacggtcg aacctagtcc
     5581 cggttctgca cctatgcgat aaacttttcc ggcaacggct tccagcacgg gtgccagtcg
     5641 ttcaaaggca atatcgctac cggaggccat gacagtcatt tcaccgttag cggcttttac
     5701 tgcaccacca gaaactggcg catccagcat ttccagatcg aatccagcca gagcggtagc
     5761 aatttcttgc gcatcagcac tagcgatagt ggaagaaacc attactgccg taccgggttt
     5821 cagatgttgt gcaacgcctg tttcaccaaa cagcacctgt ttaacctggg ccgcattgac
     5881 caccagcacc agcagagcgt ccagtttttc ggcaaacgtc gcggcgttat cagaaacccc
     5941 gcaagcacct gcctctttca acgtagcgca ggcattgctg ttcaggtctg cgccccaggt
     6001 agaaagacct gcgcggacac atgacagtgc tgctcccatt cccattgacc ctaagccaac
     6061 gataccgaca tgaaactcag atcccgtttt catctgctct ccttgttaat ttaagtgata
     6121 ttttgtttga tattgtgaat ataagcgctg gaagataacg atatggtgag ctgattcaca
     6181 taaattaaca ttgtgtgtta ttttatgtga actaagcgtt agttgcgccg cgcacgtttc
     6241 gcaggcaaat agcgtagaat gtcagcagga caaaggaagg agcaaaagtt gatacccgta
     6301 gagcgtcgcc aaatcatcct tgagatggta gctgaaaaag gcattgtcag tattgctgaa
     6361 ctaacggaca gaatgaatgt gtcacatatg accattcgtc gggatttaca aaaactggag
     6421 cagcagggag ccgttgtgct ggtgtccgga ggcgtccagt ctccgggacg cgtggcgcat
     6481 gaaccttctc atcaggtaaa aactgcgctg gcaatgacgc aaaaagcggc tattggcaag
     6541 ctggcggcaa gtcttgttca gccgggaagt tgtatctatc tggatgcggg aacgaccacg
     6601 ttagcgatag cacagcatct gattcacatg gagtcactga ctgtggtcac aaacgatttc
     6661 gttattgcga actacttgct cgacaacagt aattgcacaa ttattcacac tggcggtgca
     6721 gtgtgtcggg aaaaccgttc ctgtgtcggg gaagccgctg cgaccatgct gcgcagcctg
     6781 atgattgatc aggcttttat ttctgcatcg tcatggagtg tgcgggggat ttctacgcca
     6841 gcggaagata aagtcacggt gaaacgggcg attgccagtg ccagccgcca gcgagttttg
     6901 gtctgtgatg cgacgaaata tggtcaggtg gcgacatggc tggcgttacc attaagcgag
     6961 tttgatcaga ttattacaga cgacggtttg ccggagagtg ccagtcgcgc gctggcgaag
     7021 caggatctct ctttgctggt agcgaaaaat gaataatggc ctgcaataac atctggttac
     7081 tcatgcttca cagaagaagc atgagactac cttattttat aaaatgacag ccgcccactt
     7141 ttcggcgatc cggtatcaat ataaatctgg ttagcgaacg tctgaatgtt atcaaacatc
     7201 atatgtccaa atataaaata atcagcgccg tttatttgtt gtaactcgcc attaagcgat
     7261 ttctgcacac gatcaacagg ccagagtaat tcgctctccg ctatttcttt accaaagaga
     7321 tattcactcc ccggataatc tgcatgtgcg atgacatatt ttattatgtc gttagtgatt
     7381 tcaataatat gtggaaggtg atggaatttc agcaacagat ctattgcctc ttgttgctct
     7441 gaatcattta aatcgaaaaa ccagtcacca ccgctggcaa gccacatatt gccatcgccg
     7501 gtcgcgaacg catccagcgc cattgcttcg tggttgcctt taacggagat aaaccagggc
     7561 tggtttagca ggcgcaggac gtcaagactc tccggcccac gatcgatgtt atcgcctacc
     7621 gaaataagta agtcggtttc ggggaaaaaa gagagttgat gtaagcggga ttgtaataac
     7681 tgatattcac catgaatatc accaacgacc catatatggc gatagtgatg ggcattgatt
     7741 ttttgatagc gtgtagatgg catggtttga ccctgtaaaa taagctttcc tattatacag
     7801 ggtattttta tttgattcgt cagttgtcgt taatattccc gatagcaaaa gactatcggg
     7861 agttgttatt acaccagact cttcaagcga taaatccact ccagcgcctg acgcggagtc
     7921 agtgaatccg ggtccaggtt ttccagtgcc tcgaccgcag gcgaagtttc ttctggtacg
     7981 gacagcaaag acatttgcgt accatccact tgcgtagcgg cggcgttcgg cgaaatgctt
     8041 tccagctcac gcagcttttg ccgtgcgcgc ttaataacct cttttggcac gccggccaga
     8101 gctgcaaccg ccaggccgta gcttttgctc gccgcgccat cctgcacgct gtgcataaag
     8161 gcaatggtgt cgccgtgctc cagtgcatcg agatgcacgt tggcgacgcc ttccattttc
     8221 tccggtaact gggtcagctc gaaatagtgg gtagcaaaca gcgtcaatgc cttaatctta
     8281 ttcgccagat tttccgcgca tgcccatgcc agcgacagac catcgtaagt ggacgtcccg
     8341 cgcccgatct catccatcaa taccagactg tactcggtgg cgttatgtaa aatattggcg
     8401 gtttcagtca tctccaccat aaaggttgag cgcccggaag ccaggtcatc tgccgcgcct
     8461 acgcgggtaa agatacggtc aatcgggcca atctcgactt tttgcgccgg tacgtagctg
     8521 ccgatatagg ccatcagcgc aatcaacgcg gtctggcgca tataggtact tttaccgccc
     8581 atgttcggac cggtgatgat caacatacgg cgctgcggcg acagattcag cgggttagcg
     8641 ataaatggct cattcagtac ttgttcaact accggatggc gaccttcggt aatgcgaatg
     8701 cccggtttat caataaaggt cgggcaggtg tagttcaggg tataggcccg ttccgccagg
     8761 ttcaccagca cgtcgagttc cgccagcgcg ctcgcgctct gttgcaacgc ttccagatgc
     8821 ggcaacagca ggtcgaacag ctcttcataa agctgttttt ccagtgccag tgctttgcct
     8881 tttgaggtga gaactttatc ttcgtactct tttagctctg gaatgatgta gcgctcggcg
     8941 tttttcagcg tctggcgacg catgtagttg atgggtgcca gatggctttg cccacggctg
     9001 atttgaatgt agtagccgtg caccgcatta aagccaactt tcagcgtgtc caggccggta
     9061 cgttcacgct cgcggacttc cagacgctcc agataatcgg tcgcgccgtc agccagcgcg
     9121 cgccactcat ccagctcttc gttatagccc gatgcgataa caccaccgtc gcgtaccagc
     9181 accggcggtg tgtcgatgat tgctcgctcc agcagatcgc gcagctcggc aaactcgccc
     9241 atcttctcac gtagcgcctg taccggtgca ctatcgacag tttctaactg cgcacgcagc
     9301 tccggcagtt gctggaaagc gtggcgcata cgggccagat cgcgtgggcg agcagttcgt
     9361 aaagccagac gtgccagaat acgttccagg tcgccgacct gacgcagtac cggctgcaac
     9421 tcggcggtga aatcctgcaa tgcgccaata gtttgctggc gctcaagcaa cacgcgggta
     9481 tcgcgcactg gcatatgcag ccagcgtttc agcatacgac tacccatcgg cgtgacggtg
     9541 cagtcgagca cagaagccag cgtattttcc gcaccgcccg ccaggttctg agtaatttcc
     9601 aggttacgac gtgtcgcggc atccataatg atgctgtcct gctcacgttc catggtgata
     9661 gaacgaatat gcggcagggt cgtgcgttgg gtatctttcg catactgcaa cagacaaccg
     9721 gcagcacaaa gtccgcgcgg cgcgttctcg acgccaaaac cgaccagatc gcgggtgcca
     9781 aattgcagat tcaactgctg gcgcgcggtg tcgatttcaa actcccacag cgggcgacgg
     9841 cgcaggccgc gacggccttc aatcagcgac atctcggcga aatcttctgc atacagcagt
     9901 tccgccggat tagtgcgttg cagctctgcc gccatcgttt cgcggtcggc cggttcgctc
     9961 aggcgaaaac gcccggagct gatatccagc gtcgcgtagc cgaaaccttt gctgtcctgc
    10021 cagatagccg ccagcaggtt gtcctgacgc tcctgtaaca gggcttcatc gctgatggtg
    10081 cctggcgtaa cgatacgcac aactttgcgc tcaaccggac ctttgctggt cgccggatcg
    10141 ccaatttgtt cgcagatggc aacggactct ccctgattca ccagtttggc gagatagttt
    10201 tccaccgcat ggtagggaat ccccgccatc gggatcggct ctcccgccga agcaccgcgt
    10261 ttggtcagtg aaatatccag cagttgcgac gcgcgttttg cgtcgtcata aaacagttca
    10321 taaaaatcac ccatccggta aaacagcagg atctcgggat gctgggcttt cagcctgaga
    10381 tactgctgca tca
//
LOCUS       DAEVZA010000157        10335 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEVZA010000157 DAEVZA010000000
VERSION     DAEVZA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.157"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..376)
                     /gene="yghA"
                     /locus_tag="LAF92_004300"
     CDS             complement(<1..376)
                     /gene="yghA"
                     /locus_tag="LAF92_004300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBI9793218.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKA"
     gene            567..1061
                     /locus_tag="LAF92_004301"
     CDS             567..1061
                     /locus_tag="LAF92_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBI9793219.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(1101..2141)
                     /gene="gpr"
                     /locus_tag="LAF92_004302"
     CDS             complement(1101..2141)
                     /gene="gpr"
                     /locus_tag="LAF92_004302"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBI9793220.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            2298..3185
                     /locus_tag="LAF92_004303"
     CDS             2298..3185
                     /locus_tag="LAF92_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBI9793221.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            3304..3591
                     /locus_tag="LAF92_004304"
     CDS             3304..3591
                     /locus_tag="LAF92_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBI9793222.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            3780..4898
                     /gene="hybO"
                     /locus_tag="LAF92_004305"
     CDS             3780..4898
                     /gene="hybO"
                     /locus_tag="LAF92_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBI9793223.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            4901..5887
                     /gene="hybA"
                     /locus_tag="LAF92_004306"
     CDS             4901..5887
                     /gene="hybA"
                     /locus_tag="LAF92_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBI9793224.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            5877..7055
                     /gene="hybB"
                     /locus_tag="LAF92_004307"
     CDS             5877..7055
                     /gene="hybB"
                     /locus_tag="LAF92_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBI9793225.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            7052..8755
                     /gene="hybC"
                     /locus_tag="LAF92_004308"
     CDS             7052..8755
                     /gene="hybC"
                     /locus_tag="LAF92_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBI9793226.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            8755..9249
                     /locus_tag="LAF92_004309"
     CDS             8755..9249
                     /locus_tag="LAF92_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBI9793227.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            9242..9730
                     /gene="hybE"
                     /locus_tag="LAF92_004310"
     CDS             9242..9730
                     /gene="hybE"
                     /locus_tag="LAF92_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBI9793228.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            9723..10064
                     /gene="hypA"
                     /locus_tag="LAF92_004311"
     CDS             9723..10064
                     /gene="hypA"
                     /locus_tag="LAF92_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBI9793229.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            10077..10325
                     /gene="hybG"
                     /locus_tag="LAF92_004312"
     CDS             10077..10325
                     /gene="hybG"
                     /locus_tag="LAF92_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBI9793230.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
BASE COUNT         2357 a         2583 c         2829 g         2566 t
ORIGIN      
        1 acgccttatg cgcttcgtga accagcgaac gggcgaattt ctcatcgctt aaatcgcctg
       61 gcagcagaac ggctttgcgt ccgcattctt caatgatctt tttcacatcc tgagcgtctt
      121 cttcttccac gggaagataa ctgatcgcca cgtcagcccc ttcacgcgcg taagcgatgg
      181 cggcagcgcg acctattccg gaatcgcccc ctgtcaccag tgctttacga tctttcaggc
      241 gaccgctacc aacataggtt ttctcgccgc aatccggtac cggtgtcatc ttcgcctgga
      301 tgcctggcgt cggttgtttc tgtttgggat attcaccagt gtaatactgc gtggtcgggt
      361 cttttaaatg agacatcgtt tttctccctt caggttcaac gtcctttaag ggtagacgtt
      421 ctcgatgcgt tgataaggga accaggaaga tccctaaacc tcagaattat gcgacaaagg
      481 tttaacgtat atgttgattt gctgttgcgt gctgtttact caattgcgat atactgttgc
      541 ccgttttaac tacacgacag gaatgtatgg aacgttttct tgaaaatgca atgtatgctt
      601 ctcgctggct gcttgccccc gtgtactttg gcctttcgct ggcgttagtt gccctggcgc
      661 tgaagttctt ccaggagatt attcacgtac tgccgaatat cttctcgatg gcggaatcag
      721 atttgatcct cgtgttgctg tcgctggtgg atatgacgct ggttggcggt ttgctggtga
      781 tggtgatgtt ttccggttat gagaatttcg tctcacagct ggatatctcc gagaacaaag
      841 agaagctgaa ctggctgggg aaaatggacg caacgtcgct gaaaaacaaa gtagcagcgt
      901 cgattgtggc aatttcttcc attcacctac tgcgcgtctt tatggatgcg aaaaatgtcc
      961 cggataacaa actgatgtgg tacgtcatta tccatctgac gtttgtgctt tctgcatttg
     1021 tgatgggcta tcttgaccga ctgactcgtc ataatcactg atctgattcg ggcgcggttc
     1081 gcgcgcccgt tattaacagg tcatttatcg gacgacgcct gccacagatt cagctcgcca
     1141 tcggcgatat gctgatcaat ctgcgccagc tcctcggtgc taaatgtcag attattcagc
     1201 gcctgcacgt tctcctctag ttgctccgcg cggctggcac caatcaatac cgacgtcacg
     1261 cgatcatctt tcagcaacca gcttaacgcc atttgcgcca ttgattgtcc acgctgctgt
     1321 gccatttcat tcaataagcg caggctgttg aggttggctt cggtaagcat tttcggtgtc
     1381 agaccacgaa ctttattccc ttcacgatac atccgtgaat cttgcggaat gccgttgaga
     1441 tattttccgg tcagcaatcc ctgagccaga ggagtaaagg caatacagcc cacgccgtta
     1501 ttttgcaggg tatccagcag gccgctttta tccacccagc ggttcagtaa attgtacgaa
     1561 ggttgatgaa ttaaaagcgg aattttccac tcgtgcagca actcgaccat tttttgcgtc
     1621 cgctctggcg agtaagagga gatcccgaca tacagcgcct taccgctttg taccgcatga
     1681 gccagcgcag aggcggtttc ttccatcggc gtattttcat cgacgcgatg agagtaaaag
     1741 atatcgacat actcaagccc catacgcttc aggctttggt cgaggctggc gagcaggtat
     1801 ttacgtgaac cgccagagcc gtaagggccg ggccacatat cgtagccagc cttggtagag
     1861 ataatcagtt catcgcgata agcggcaaaa tcctcccgca gcaggcgacc aaagttctct
     1921 tctgcgcttc ctggaggcgg cccgtaattg ttggctaaat caaagtgcgt aatgcccaaa
     1981 tcaaacgctt tacgcaggat cgcacgctgt gattccagcg cgttaacgtg accgaaattg
     2041 tgccataaac cgagcgataa cgcgggcagg cgtaaaccgc tttttccgca atagcggtat
     2101 tgcatctgcc cgtaacgttc gggattcgct aaccagacca tgacctctcc tttccaccgt
     2161 tcaatttcga aacaatgttt ctagtttagc gattcgccag cgcgtatccc gtagtctggc
     2221 tcacagagtg acgaaaaact gggcaaaaac acgtgcttat gctttgctta aaaaaacacc
     2281 agttgaggag tgcaacgatg ccgcgtttaa ccgccaaaga tttcccacaa gagttgttgg
     2341 attactacga ctattacgct cacgggaaaa tctcgaaacg tgagttcctc aaccttgcgg
     2401 cgaagtatgc ggtgggcggg atgacggcat tagcgttgtt tgatttgctc aagccaaatt
     2461 atgcgctggc gactcaggta gagtttaccg acccggagat tgttgctgag tacatcacgt
     2521 atccttcgcc aaatggtcac ggcgaggtac ggggttatct ggtgaaaccc gcaaaaatga
     2581 gcggcaaaac gccagccgtg gtagtggtgc atgagaatcg tggactgaat ccgtatatcg
     2641 aagatgtggc acggcgagtg gcgaaggcgg ggtatatcgc cctggcacct gacggcttaa
     2701 gttccgttgg aggttatccg ggaaatgatg ataaaggtcg tgagctgcaa cagcaggttg
     2761 atccaaccaa actgatgaat gatttctttg ccgcaattga gtttatgcaa cgctatccgc
     2821 aagcggcagg caaagtgggt attaccggat tttgctatgg cggtggcgta tcgaacgcgg
     2881 cggctgtcgc gtatccggaa ctggcctgcg cggtgccgtt ttatggtcgt caggcaccca
     2941 ctgccgatgt ggcgaagatt gaagcgcctt tactactcca cttcgcggaa ctggacaccc
     3001 gaatcaacga gggctggcct gcttacgagg cggcgttgaa agccaataat aaggtttatg
     3061 aggcgtatat ctatccgggg gttaatcacg gattccataa tgattccacg ccccgttatg
     3121 acaaatctgc cgccgatctt gcctggcaaa ggacactgaa atggttcgat aaatatctct
     3181 cctgataggt ttatctctta ctggattacg tcttaaacaa gcatgaaaaa atagcgtgcg
     3241 caaaagtcgt tctttgccta aaatatcgct atatatggcg atatatagcg aatgaggtga
     3301 acgatgaata accattttgg taaaggctta atggcgggat taaaagcaac gcatgccgac
     3361 agtgcggtta atgtgacaaa attctgtgcc gattataaac gcggttttgt attaggctac
     3421 tcacaccgga tgtacgaaaa gaccggagat cgccagctta gcgcctggga agcgggtatt
     3481 ctgacgcgcc gctatggact ggataaagag atggtaatgg atttctttcg tgagaataat
     3541 tcctgttcta cgttgcgctt ttttatggcc ggttatcgcc tcgaaaattg atcaaacata
     3601 cgtattatct tgctttaatt aattacacta atgcttcttc ccttcgtttt agcgccccgc
     3661 cgcagtatca tgatatcgat aaccataata aatgtgtggt aaatggcgca tcgatcgcat
     3721 tattgatttt gcgattgagg caaaatatat gccaggtctt cgcaacggaa taactataaa
     3781 tgactggaga taacaccctc atccattctc acggcattaa ccgtcgtgat ttcatgaagc
     3841 tttgtgcagc attagccgcc accatggggt taagtagcaa agccgctgca gagatggccg
     3901 aatcggttac taacccgcag cgtccgccag ttatctggat tggcgcgcag gagtgcaccg
     3961 gttgtacgga atctctgctt cgtgcaacgc atccaacggt agaaaacctc gtgctggaga
     4021 ctatctctct ggagtatcac gaagtgcttt ccgccgcctt cggtcatcag gtcgaagaga
     4081 acaaacataa cgcacttgag aagtacaaag ggcagtatgt gttggttgtg gatggttcca
     4141 tcccattaaa agataacggt atttattgca tggttgctgg tgagccgatt gtggatcaca
     4201 tccgcaaagc ggcagaaggc gcagcagcga ttatcgctat cggttcctgc tctgcgtggg
     4261 gcggtgttgc cgcagctgga gttaacccaa ctggcgcagt cagcctgcaa gaagttctgc
     4321 caggcaaaac cgttatcaat attccgggct gcccgccgaa cccgcacaac ttcctcgcga
     4381 ccgttgcgca catcatcact tacggcaaac cgccgaaact ggatgacaaa aatcgtccga
     4441 ccttcgccta tggccgtctg attcacgaac actgcgaacg tcgcccgcac ttcgatgctg
     4501 gtcgttttgc caaagagttc ggtgatgaag gccaccgtga aggctggtgc ctgtaccacc
     4561 tcggctgtaa agggccagaa acttacggca actgctcaac gctgcaattc tgcgatgttg
     4621 gcggcgtgtg gccggtagcg attggtcacc catgctatgg ctgtaacgaa gaaggtatcg
     4681 gcttccataa aggcatccat cagcttgcca acgtcgaaaa tcagactccg cgttcacaga
     4741 aaccggatgt taacgctaaa gagggcggca acgtctctgc aggcgctatt ggtttgctcg
     4801 gcggtgtggt tgggctggtt gccggtgtca gcgtgatggc ggtgcgtgaa ctgggtcgtc
     4861 agcaaaagaa agataacgct gactcacggg gagaataacc gtgaacagac gtaattttat
     4921 taaagcagcc tcctgcgggg cattgctgac gggcgcgctg ccgtctgtca gtcatgcggc
     4981 tgctgaaaac cgcccgccaa ttccgggatc gctggggatg ttgtacgact cgaccttgtg
     5041 cgtaggctgc caggcttgcg tcaccaagtg tcaggatatc aatttccctg aacgtaaccc
     5101 gcaaggggaa cagacctggt cgaacaacga caaactgtcg ccgtatacca ataacatcat
     5161 tcaggtgtgg accagcggca caggggtcaa caaagaccag gaggagaacg gctacgcgta
     5221 cattaagaaa cagtgtatgc actgcgtcga tccgaactgt gtctctgtgt gcccggtctc
     5281 tgcactgaaa aaagatccga aaaccggcat tgtccattac gacaaagacg tgtgcaccgg
     5341 ttgccgttac tgcatggtcg cctgtccgta caacgtgccg aagtacgact acaacaaccc
     5401 gtttggtgcg ctgcataagt gcgagctgtg caaccagaaa ggtgtggaac gtctcgataa
     5461 aggcggtctg cctggctgcg tagaagtgtg cccggcgggc gcggtgattt tcggtacgcg
     5521 tgaagagctg atggcggagg cgaaaaaacg tctggcgctg aagcctggca gcgaatacca
     5581 ctatccgcgt cagacgctga aatctggcga cacttacctg catacggtgc cgaaatatta
     5641 tccgcatctg tacggcgaga aagagggcgg cggtactcag gttctggtac tgacgggtgt
     5701 gccttatgaa aatctcgacc tgccgaaact ggacgatctt tctaccggtg cgcgttccga
     5761 aaatattcaa cacaccctgt ataaaggcat gatgctacca ctggctgtgc tggcgggctt
     5821 aaccgtgctg gttcgtcgca acaccaaaaa cgaccatcac gacggaggag acgatcatga
     5881 gtcatgatcc acaaccgctg ggcggcaaaa tcatcagtaa accggtcatg atttttggac
     5941 cgttaatcgt catctgtatg ctcctgattg tgaagcgtct ggtgttcggt ctgggctctg
     6001 tctctgacct gaacggcggc ttcccgtggg gcgtgtggat cgcgtttgac ctgctgattg
     6061 gcaccggctt tgcctgtggc ggctgggcgc tggcgtgggc ggtatacgtc tttaaccgtg
     6121 ggcaatacca tccgctggtg cgtccggcgc tgttggcgag tctgtttggt tactcactgg
     6181 gtggcttgtc gatcactatc gacgtgggtc gctactggaa cctgccgtac ttctacattc
     6241 cgggtcactt caacgtgaac tcggtactgt tcgagacggc ggtctgtatg accatctata
     6301 tcggcgtgat ggcactggag tttgctccgg cactgtttga acgtctgggg tggaaggtgt
     6361 cgctacagcg actaaacaag gtgatgttct tcatcatcgc gctcggtgcg ctgctgccga
     6421 ccatgcacca gtcttcaatg gggtcgctga tgatctcggc gggctacaag gtgcatccgt
     6481 tgtggcagag ctatgaaatg ttgccgctgt tctcgttgct gacggcgttc atcatgggct
     6541 tctcgattgt catctttgaa ggttcgctgg tgcaggcggg tctgcgtggc aacggtccgg
     6601 atgaaaagag tctgtttgtt aagctgacca acaccatcag tgtgttgctg gcgattttca
     6661 tcgtgctgcg ctttggcgag cttatctatc gcgacaagct gtcgttagcg tttgccggtg
     6721 acttctactc cgtgatgttc tggattgaag tcctgctgat gctcttcccg ctggtcgttc
     6781 tgcgtgtggc gaagctgcgt aatgattccc gcatgctgtt cctgtcagca ctgagcgcac
     6841 tgttaggttg tgcaacctgg cgtctgacct attcgctggt ggcattcaat ccgggcggcg
     6901 gttacgccta cttcccgacc tgggaagaac tgttgatttc tattggtttt gtggctattg
     6961 agatttgcgc ttacatcgta ctcattcgtc tactgccgat acttcctcct ttaaaacaaa
     7021 acgatcataa tcgtcatgag gcgagcaaag catgagccag agaattacta ttgatccggt
     7081 aacccgtatt gaggggcatt tacgcatcga ttgcgaaatc gaaaatggcg tcgtttcgaa
     7141 agcatgggct tccggtacca tgtggcgcgg catggaagag atcgtgaaaa accgcgatcc
     7201 gcgcgatgca tggatgattg tgcaacgtat ctgtggcgta tgtactacca ctcacgcgct
     7261 gtcttccgtt cgtgcggcag aaagtgcgct gaatatcgac gttccggtta acgcgcaata
     7321 catccgtaac atcattctgg ctgcgcacac cacgcatgac catatcgttc atttctatca
     7381 gctttcggcg ctggactggg tggacatcac ttctgcactg caagctgacc caaccaaagc
     7441 ctccgaaatg ctgaaaggcg tttcgacctg gcatctgaac agtccggaag agttcactaa
     7501 agttcagaac aagatcaaag atctggttgc cagcggtcag ttgggtattt tcgctaatgg
     7561 ctactggggt cacccagcga tgaaactgcc gccggaagtg aacctgattg cggtagcgca
     7621 ctacctgcag gcgctggagt gccagcgtga cgctaaccgc gtcgtggcgc tgctgggcgg
     7681 taaaacgccg cacattcaga acctggcagt gggtggtgtc gcgaacccaa tcaacctcga
     7741 cgggttgggc gtgttgaacc ttgagcgcct gatgtacatc aagtctttca tcgacaaact
     7801 gagcgacttt gttgagcagg tttataaggt tgataccgcg gttatcgccg cgttctaccc
     7861 ggaatggctg acgcgcggta aaggtgcggt gaactacctg agcgtgccgg aattcccgac
     7921 cgacagcaaa aacggcagct tcctgttccc gggcggctac attgagaatg cggatctgtc
     7981 ctcgtatcgt ccgatcactt ctcattccga tgaatacctg atcaaaggca ttcaggaaag
     8041 cgcgaagcac tcctggtata aagacgaagc gccacaggcg ccgtgggaag gtaccaccat
     8101 tccggcttat gatggttggt ctgacgacgg gaaatattcc tgggtgaaat caccgacttt
     8161 ctacggcaaa acggtagaag tggggccgct ggctaatatg ctggtgaaac tggcggcagg
     8221 tcgagaatct acccagaaca aactgaatga aatcgttgcg atttatcaga aactgactgg
     8281 caacacgctg gaagtggcac agctgcactc cacgctgggc cgtattattg gtcgtaccgt
     8341 tcactgctgt gaattgcagg atatcctgca aaaccaatac agtgcactga tcaccaatat
     8401 cggcaaaggc gatcacacca cctttgtgaa accgaacatt ccggcaacgg gtgagttcaa
     8461 aggtgttggc ttcctcgaag cgccgcgcgg tatgctctct cactggatgg tgatcaaaga
     8521 cggtatcatc agcaactacc aggcagttgt tccatcaacc tggaactctg gtccgcgtaa
     8581 cttcaatgat gacgtcggtc cttacgagca gtcgctggtg ggtacaccgg ttgctgatcc
     8641 gaataaaccg ctggaagtgg tgcgtaccat tcactccttc gacccgtgca tggcctgtgc
     8701 ggtacacgta gtggatgccg acggcaacga agtggtttca gtgaaggttc tgtaatgcgt
     8761 attttagtct taggggtcgg caatattttg ctgaccgatg aagccatcgg tgtgcggatt
     8821 gtcgaagcgt tagagcaacg atacattctg ccggattatg ttgagatcct cgatggcggc
     8881 acggcgggaa tggagctgct tggcgacatg gcaaatcgcg atcatctgat tattgcggat
     8941 gccattgtgt cgaagaagag cgcgccggga acgatgatga tcctgcggga tgaagaagtt
     9001 ccggcgttgt ttaccaacaa aatctctccg catcagcttg gcctggccga cgtcttgtcg
     9061 gccctgcgct tcaccggcga gtttccgaaa aagctgaccc tggtcggcgt gatcccggaa
     9121 tcgctggagc cacacatcgg cttaacgccg acggttgaag caatgattga acctgcgctt
     9181 gagcaggttc tggctgcgct gcgcgaatca ggcgtggaag ccatcccacg ggaggcgatt
     9241 catgactgaa gagatagcag gtttccagac ctccccgaag gcgcaagtac aggcagcgtt
     9301 tgaagaaatt gcccggcgtt cgatgcacga tctctctttt ctgcatcctt caatgccggt
     9361 gtatgtttct gattttacgc tgttcgaagg tcagtggacg gggtgtgtga tcaccccgtg
     9421 gatgctgagt gcagttatct tccccggccc ggatcaactc tggccgctgc gcaaagtgag
     9481 tgaaaaaatt ggtctgcaac tgccgtatgg caccatgacc tttaccgttg gcgaactgga
     9541 cggtgtttcg caatacctct cctgttcgct gatgtcaccg ctttcgcaca gtatgtcgat
     9601 tgaagagggc caacgcctga cggatgactg cgcacgaatg atcctttcgc tgccagtcac
     9661 gaatccggat gtaccacacg cagggcgtcg cgccctgctg tttggtcgca ggagtggcga
     9721 aaatgcatga gttgtcgctt tgccagagcg ccgttgaaat tatccaacgg caggcggagc
     9781 agcacgatgt taagcgcgtc accgccgtgt ggctggaaat tggcgcgctc tcctgcgttg
     9841 aggagagcgc cgtccgtttt agttttgaaa ttgtctgcca cggaacggtg gcgcaagggt
     9901 gcgatttaca tatcgtctat aaacccgccc aggcctggtg ctgggattgc agccaggtgg
     9961 tggagattca tcagcacgat gcgcagtgtc cgatctgtca tggcgagcgg ttgcgtgtcg
    10021 ataccggcga ttcgctgatc gtcaaaagta ttgaagttga ataaccggag ttaataatgt
    10081 gtattggcgt tccaggccag gtgctggctg tcggtgaaga tattcaccag cttgcgcagg
    10141 ttgaagtatg tggtatcaag cgcgacgtga atatcgccct gatttgtgaa ggtaaccctg
    10201 ccgatctact gggccagtgg gtgctggtac acgtcggatt tgccatgagc atcatcgacg
    10261 aagatgaagc caaagccaca ttagacgcac tgcgccaaat ggattacgac attaccagcg
    10321 cgtgatgatt agctt
//
LOCUS       DAEVZA010000158        10207 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEVZA010000158 DAEVZA010000000
VERSION     DAEVZA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.158"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            6..755
                     /locus_tag="LAF92_004313"
     CDS             6..755
                     /locus_tag="LAF92_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793231.1"
                     /translation="MECVASSNSAIPNVVEVIRRINEGSTQPFLCKCDDGQLYVLKSK
                     PSMPPKNLLAEFISACLANDIGLPLPDFKIVFVPEELIEYSPDLQQQICTGYAFASLF
                     IDGAIALTFTQSRNETIIPVEQQKLIYVFDKWILNADRTLTDKGGNVNILYDISNDKY
                     YLIDHNLSFDQNAGPEDFSVHVYGPGNRKWQYDLVDRVEYRQRVVNSLHKLPAILDEI
                     PEEWIVDEEFLPFVCTTLDKGDCDEFWSAIE"
     gene            752..1579
                     /locus_tag="LAF92_004314"
     CDS             752..1579
                     /locus_tag="LAF92_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HBI9793232.1"
                     /translation="MTTPCLYSIVRYAPYAETEEFANIGVLLCAPKENYFDFQLTKRN
                     DSRVKNFFHDDCIFPVAKDSIQRELQFAKMHATQIVGHQQLAQFFRYFTNKKESIFQF
                     SSTRVILSENPKEELARIYNKYVNHSDYTKERREDVLARELKRSIDRIDGLKNVFKQA
                     TIDGYFAKFSMPLVAKKHDRIQCAIKPLAFTQAEPGKMMEHSDTWVMRITRAAEENLL
                     SLDDILFTIETPESPNSGQSKVIDIIKRTMDAKKINHIPASNHKETIDFAKKILPQV"
     gene            2088..2294
                     /locus_tag="LAF92_004315"
     CDS             2088..2294
                     /locus_tag="LAF92_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HBI9793233.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            2371..2667
                     /locus_tag="LAF92_004316"
     CDS             2371..2667
                     /locus_tag="LAF92_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBI9793234.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDLEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            2673..3458
                     /gene="bet"
                     /locus_tag="LAF92_004317"
     CDS             2673..3458
                     /gene="bet"
                     /locus_tag="LAF92_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HBI9793235.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKASEQKVAA"
     gene            3455..4135
                     /locus_tag="LAF92_004318"
     CDS             3455..4135
                     /locus_tag="LAF92_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBI9793236.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVIE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFIKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDENYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            4132..4314
                     /locus_tag="LAF92_004319"
     CDS             4132..4314
                     /locus_tag="LAF92_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HBI9793237.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPSLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            4287..4478
                     /locus_tag="LAF92_004320"
     CDS             4287..4478
                     /locus_tag="LAF92_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HBI9793238.1"
                     /translation="MHKASPVELRMSIEMAHSLAQIGVRFVPIPAETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            4555..4770
                     /locus_tag="LAF92_004321"
     CDS             4555..4770
                     /locus_tag="LAF92_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBI9793239.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            4869..5087
                     /locus_tag="LAF92_004322"
     CDS             4869..5087
                     /locus_tag="LAF92_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBI9793240.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEEIELKNKQWGL"
     gene            5135..5374
                     /locus_tag="LAF92_004323"
     CDS             5135..5374
                     /locus_tag="LAF92_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBI9793241.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRAIRRK"
     gene            5514..5750
                     /locus_tag="LAF92_004324"
     CDS             5514..5750
                     /locus_tag="LAF92_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBI9793242.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            5740..6882
                     /locus_tag="LAF92_004325"
     CDS             5740..6882
                     /locus_tag="LAF92_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HBI9793243.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(6996..8246)
                     /gene="icd"
                     /locus_tag="LAF92_004326"
     CDS             complement(6996..8246)
                     /gene="icd"
                     /locus_tag="LAF92_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBI9793244.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAGKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            8418..9071
                     /gene="rluE"
                     /locus_tag="LAF92_004327"
     CDS             8418..9071
                     /gene="rluE"
                     /locus_tag="LAF92_004327"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBI9793245.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            9081..9542
                     /gene="nudJ"
                     /locus_tag="LAF92_004328"
     CDS             9081..9542
                     /gene="nudJ"
                     /locus_tag="LAF92_004328"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBI9793246.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            9596..>10207
                     /gene="mnmA"
                     /locus_tag="LAF92_004329"
     CDS             9596..>10207
                     /gene="mnmA"
                     /locus_tag="LAF92_004329"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBI9793247.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGER"
BASE COUNT         2896 a         2409 c         2408 g         2494 t
ORIGIN      
        1 tagtgatgga gtgcgtagcg agcagcaata gtgcaatacc taatgtcgtt gaagtaatac
       61 gtcgcatcaa tgaaggttcc actcagccat ttctttgcaa atgtgatgat gggcagttgt
      121 atgttttgaa gtcaaaacca tcaatgcccc cgaaaaatct cttagctgag ttcatttcgg
      181 cgtgtttggc taatgatatc ggccttcctt tacctgactt taaaatcgta tttgtgccag
      241 aggaacttat agagtactca cctgatctgc agcaacaaat ttgtacagga tatgcctttg
      301 cttcattgtt cattgacggt gcaatagcgt taacgtttac gcagtcaaga aacgaaacga
      361 tcatcccagt cgaacagcaa aaattaatct atgtttttga taaatggata ttaaatgcag
      421 acagaacgct tactgacaaa ggtggaaacg ttaacatcct ttatgacatc agtaacgata
      481 agtattatct gattgaccat aatctctcat ttgatcagaa tgctggacct gaagattttt
      541 ctgtgcacgt gtacggccct ggtaaccgca aatggcaata tgatttagtg gatcgcgtag
      601 agtaccgcca gagggtcgtt aacagtttac acaagcttcc tgctatcctt gacgaaattc
      661 cagaagagtg gatagtagat gaggagtttt taccttttgt ctgcactacg ctagacaaag
      721 gtgattgtga tgaattttgg agcgcaatag aatgacaact ccatgcctat atagcatcgt
      781 tcgctatgcg ccttatgcgg agactgaaga gttcgcaaac ataggcgtac ttctgtgcgc
      841 gccaaaagaa aattactttg atttccagct cacaaagcga aatgactctc gtgtaaagaa
      901 ttttttccat gatgattgta ttttccctgt agcaaaagac tcaatacaaa gagaactaca
      961 gttcgcaaaa atgcatgcga cccagattgt tggacatcaa caacttgcac aattcttcag
     1021 atattttaca aacaaaaaag aatcaatttt tcagttcagt tctacgagag tgattctcag
     1081 cgaaaaccca aaagaagagc tggcccgcat ttacaataaa tatgtaaacc actctgacta
     1141 cacaaaagag cgccgtgaag atgttctagc cagagagcta aaacgaagta tcgatagaat
     1201 agatggattg aagaacgtct tcaaacaagc aaccattgat gggtatttcg caaagttctc
     1261 aatgccattg gtcgccaaga agcatgacag gatccaatgt gccatcaaac ctctggcatt
     1321 cactcaagct gaaccaggaa aaatgatgga gcatagtgat acttgggtga tgagaataac
     1381 tcgagcagca gaagaaaacc tgctttcact tgatgacatt ttattcacaa ttgaaactcc
     1441 tgaatcacca aactcaggcc aaagcaaagt tattgacatc ataaagagaa ctatggatgc
     1501 taagaaaata aatcatatac ctgcatccaa ccacaaagaa actattgatt ttgcaaaaaa
     1561 aatacttccc caagtttaaa atttattttt gtatgtgata ttccttatta ataacccggc
     1621 caccgtgccg ggttttcttt tgcctcccct catcacacaa accgctcaaa aaaccaccat
     1681 aacctcgctt cagttatcgc tatgcgattc aagtcacaaa ataaatccat cctaaataca
     1741 accagttata tctaaaacaa ccaataaaac aacgtttgtt gttgacggta aaacaactat
     1801 agttttaaat aggttcatcg caacaacaca acgatacggc aaccacctga ttcaccgttg
     1861 cgatgaccgc ttagatccgc agtttgaatt tcagcaggct tcggggagtg cgaggggtga
     1921 aacggacgcg tgaacgtcgg tgtgaccagc tgaaatcaac tcaacatttc ataccttagt
     1981 cgcttcaacg aggcggctta gttatgacaa ccggcggcca tccaccgcct gaatacgcgc
     2041 agaagtctct atatgttcag cagcccagct tacgggcagg agtttttatg gttcatcaac
     2101 attacggaac gcagaccgtt aatcgcggcg cggtcatgcc aggaatgctg gtcaaacaca
     2161 aagatggtac ctggactgca tcagctaatt tacgcggacg gctttatctg catcgcggca
     2221 tcgagcgcac ttatacccgt gatttgctcg tggaagtttt tctcgacgga cgcggtaacg
     2281 gcctgaatca ctaacccccc tttcctgttt tcctaatcag cctggcattt cgcgggcgct
     2341 attttcacag ccattttcag gagttcagcc atgaacgctt attacattca ggatcgtctt
     2401 gaggctcaga gctgggcgcg tcactaccag cagatcgccc gtgaagagaa agaggcagaa
     2461 ctggcagacg acctggaaaa gggtctgccc cagcatttgt ttgaatcgct ctgcatcgat
     2521 catttgcaac gtcacggggc cagcaaaaaa gccattaccc gtgcgtttga tgacgatgtt
     2581 gagtttcagg agcgcatggc agaacacatc cggtacatgg ttgaaaccat tgctcaccac
     2641 caggttgata ttgattcaga ggtataaaac ggatgagtac agcactcgca acgctggctg
     2701 ggaagctggc tgaacgtgtc ggcatggatt ctgtcgaccc acaggaactg atcaccactc
     2761 ttcgccagac agcatttaaa ggcgatgcca gcgatgcgca gttcatcgca ttgctgatcg
     2821 tcgccaacca atacggcctt aatccgtgga cgaaagaaat ttacgccttc cctgataagc
     2881 agaacggcat cgttccggtg gtgggcgttg atggctggtc ccgcatcatc aacgaaaacc
     2941 agcagtttga cggcatggac tttgaacagg acaacgaatc ctgcacatgc cggatttacc
     3001 gcaaagaccg caatcatccg atctgcgtta ccgagtggat ggatgaatgc cgccgcgaac
     3061 cattcaaaac ccgcgaaggc agagaaatca ccggaccgtg gcagtcgcat cccaaacgga
     3121 tgttacggca taaagccatg attcagtgtg cccgtctcgc cttcggattt gcgggtatct
     3181 atgacaagga tgaagccgag cgcattgtcg aaaataccgc atacactgca gaacgtcagc
     3241 cggaacgcga catcactccg gttaacgatg aaaccatgca ggagattaac actctgctga
     3301 ttgccctgga taaaacatgg gatgacgact tattgccgct ctgttcccag atatttcgcc
     3361 gcgacattcg cgcatcgtca gaactgacac aggccgaagc agtgaaagct cttggattcc
     3421 tgaaacagaa agcctctgag cagaaggtgg ctgcatgaca ccggacatta tcctgcagcg
     3481 taccgggatc gacgtgagag ctgtcgaaca gggggatgat gcatggcaca aattacggct
     3541 cggcgtcatc accgcttcag aagttcacaa cgtgatagca aagccccgat caggaaagaa
     3601 gtggcctgac atgaaaatgt cctacttcca caccctgctg gctgaggttt gcaccggtgt
     3661 ggctccggaa gttaacgcta aagcgctggc ctggggaaaa cagtacgaga acgacgccag
     3721 aaccctgttt gagttcactt ccggcgtaaa tgttattgaa tccccgatca tctatcgcga
     3781 cgaaagtatg cgcaccgcct gctctcccga tggtttatgc agtgacggca atggccttga
     3841 gctgaaatgc ccgtttacct cccgggattt cattaagttc cggctcggtg gtttcgaggc
     3901 cataaagtcg gcttacatgg cccaggtgca gtacagcatg tgggtgacgc gaaaagatgc
     3961 ctggtacttt gccaactatg acccgcgcat gaagcgtgaa ggcctgcatt atgtcgtggt
     4021 tgagcgggat gaaaattaca tggcgagttt tgacgagatg gtgccggagt tcatcgaaaa
     4081 aatggacgag gcactggctg aaattggttt tgtatttggg gagcaatggc gatgaagcat
     4141 cctcacgata atatccgggt aggtgcgatc actttcgtct actccgttac aaagcgaggc
     4201 tgggtatttc ccagcctttc tgttatccaa aatccactga aagcccagcg gctggctgag
     4261 gagataaata ataaacgagg ggctgtatgc acaaagcatc tcctgttgag ttaagaatga
     4321 gcattgagat ggcacatagc cttgctcaaa ttggagtcag gtttgtgcca ataccagcag
     4381 aaacagacga agaatttcat acgttagcca catccctttc acaaaagctg gaaatgatgg
     4441 tggcgaaagc agaagcagat gagagagacc aggtatgaca accactgaat gcatttttct
     4501 ggcagcgggc ttcatattct gtgtgcttat gcttgccgac atgggacttg ttcaatgaca
     4561 cctcagcaag aaaacgccct tcgcagtatt gcccgtcagg ctaattctga aatcaaaaaa
     4621 gccagacagc agtttccgga taaaaacgtc gatgacattt gccgtagcgt actgaagaag
     4681 caccgcgaaa cggtaacgct gatgggattc acaccgactc acttaagtct ggcaatcggc
     4741 atgttaaacg gcgtctttaa ggaacggtga acatgaaaag caaaatcatc agggagctac
     4801 aggctccttt tttattattc gcattcaccc tcaagcgtat taaccaacaa ttcagggatt
     4861 aatggaagat ggcagacatc attgattcag catcagaaat cgaagaatta cagcgcaata
     4921 cagcaataaa aatgcgtcgt ctgaactacc agactgtatc tgccactcat tgttgtgagt
     4981 gtggcgatcc gatagatgag cgaagacgcc tggtcgttca gggttgtcgg acttgtgcaa
     5041 gttgccagga ggagatcgaa cttaagaaca aacaatgggg attgtgatgg cctcaaagca
     5101 gcaaatttca acatcgtcca actgaggtgt aaaaatgttc agaatcattt ttcctaacac
     5161 ctggtacgtc gaccaccacg gcactccctg caaaatcctg cgttctaccc acaacaaagt
     5221 tcactacatc cgaaaaggca gaacatgtat cgccagcatg ttccgcttta atcatgactt
     5281 tgaacctgtg aataaagctg atgcagatcg gatagcagaa gagatcgaaa cggcagaaca
     5341 cattaagaag ttacgtgcca tacgcaggaa atagaaaaat tgataaattc aatactgcat
     5401 ttctcagcat taaatttatc tctatgacca gtcaagagat gtacctgcca tgagcttaat
     5461 atcatgtcag atatatcggt cacaaactcc ctcagcagct aagaggagga caaatgtctc
     5521 gactaatcac tttacaggac tgggctaaag aagaatttgg ggacttagca ccaagtgagc
     5581 gagttctgaa aaaatacgcg caagggaaaa tgatggcccc acccgctata aaagttggtc
     5641 gctactggat gattgaccga aattcccgtt ttgtaggaac gcttgcagaa ccgcaactcc
     5701 caataaacgc aaacccaaaa ctccaacgga taatcgctga tggctgctag accccgatct
     5761 cacaaaatct ctatacccaa tttatattgc aaattagata agcgaaccgg aaaggtatat
     5821 tggcaataca aacatccact atccggtcgt tttcatagct taggaactga tgagaatgaa
     5881 gcaaaacaag ttgctactga agcaaatacc attattgctg aacaacgtac ccgacaaata
     5941 ttaagcgtca atgagcgtct ggaaagaatg aaaggcaggc gctcagacat tacggtgaca
     6001 gaatggcttg ataaatatat ttctatccag gaggacaggc tgcaacataa tgaactaaga
     6061 cccaactcct atcggcaaaa aggcaaaccc attcgtcttt tccgtgagca ttgtggaatg
     6121 caacacctca aggatattac cgcacttgat attgccgaaa taattgatgc tgtaaaggct
     6181 gaaggtcata acaggatggc gcaagtcgtg agaatggtgt tgatcgacgt cttcaaagaa
     6241 gcacaacacg caggacatgt tccgccagga tttaacccag cgcaggcaac aaaacaaccg
     6301 cgaaatcgag taaaccgcca aagattatca ctgcccgaat ggcaggcaat atttgacagc
     6361 gtaagcagac gacagcccta tttaaaatgc gggatgctac ttgctcttgt cactggacaa
     6421 cgtttaggcg atatctgcaa tttgaaattc tctgatatct gggacgacat gttgcacatt
     6481 actcaggaaa aaaccggttc aaaacttgct attccgctta acctgaaatg cgatgctctg
     6541 aatattaccc ttcgtgaagt tatatctcag tgcagggatg ctgttgttag taaatatctg
     6601 gtccattacc gtcacactac ctctcaagca aacagaggag accaggtttc tgcaaatact
     6661 ctgacaacgg cttttaaaaa ggcccgggaa aaatgtggca taaaatggga gcaaggaact
     6721 gcgcccacat ttcatgaaca gcgatctctg tcagaacggt tatatcggga acagggtctg
     6781 gatacgcaaa agttgttagg ccataaatcc agaaaaatga ccgaccgata caatgatgat
     6841 cgtggtaaag actggattat cgtagatatc aaaacagcat agaaaatagc cagttttggg
     6901 gaagggtttt ggggaaagtt ttggggaaga ttttacatca tcataaaaca acgggcgtgt
     6961 tatacgcccg tttcaatatt taacacatgt agtgattaca tattcttgat gatcgcgtca
     7021 ccaaactctg aacatttcag cagtttagcg ccttccatca gacgttcgaa gtcataggtt
     7081 acggtcttgg cattgattgc gccttccata cctttaacaa tcaggtcagc cgcttcagtc
     7141 caacccatat ggcgtaacat catctcagcg gagagaataa tagagccagg gtttactttg
     7201 tcctgaccgg cgtatttcgg cgcagtaccg tgggtggctt caaacagggc gcattcgtca
     7261 ccgatgtttg caccaggggc gataccgata ccgccaacct gcgctgccag ggcgtcagaa
     7321 atgtagtcac cgttcaggtt catacaggcg ataacatcat attcagccgg acgcagcagg
     7381 atctgttgca ggaatgcatc agcaatcacg tctttaatga cgatctcttt gccggtgttc
     7441 gggtttttaa ctttcagcca cgggccgccg tcgatcagtt caccgccaaa ctcttcacgc
     7501 gccagctggt agccccagtc tttaaacgcg ccttcggtga acttcatgat gttgcctttg
     7561 tgcaccaggg tcacagagtc acggtcgttg gcaattgcgt attcaatcgc ggcacggacc
     7621 agacgtttgg tgccttcttc agaacacggc ttgataccga taccgcaatg ttccgggaag
     7681 cgaattttct tcacgcccat ctcttcacgc aggaatttaa tcactttccc ggcgtcggca
     7741 gagtctgctt tccattcgat acccgcataa atgtcttccg agttttcacg gaagataacc
     7801 atatcggtca gttcagggtg tttaaccggg cttggagtgc cttgatagta acgtaccgga
     7861 cgcaggcaga tgtagagatc cagttcctgg cgcagggcaa cgttcagaga gcgaataccg
     7921 ccaccaacag gagtggtcag cggaccttta atggcaacgc gatattcacg aatcagatca
     7981 agggtttcag caggcagcca gacatcctga ccataaacct gtgtggattt ttcaccggtg
     8041 taaatttcca tccaggagat tttacgctcg cctttatagg ctttctcgac tgcagcgtcg
     8101 accactttca gcatggctgg ggttacatct acaccgattc catcaccttc aatgtaaggg
     8161 ataatcggat tttcaggaac gttgagtttg ccgttttgca gggtgatctt cttgccttgt
     8221 gccggaacaa ctactttact ttccattcac ctctccttcg agcgctactg gtttgctcgt
     8281 ctgccaccac gttgcatatg cgtttgcgtc ctgcgatacg gatgctttag agcaattttt
     8341 tgttaatgat ttgtaattgg cttgtcaata ctaccctatt gtttgccgtc atgaaagatg
     8401 cgcgttatta ggctataatg cggcaattca taatctctga aaataccatg caaaaaactt
     8461 cttttagaaa tcaccaggtt aagcgattca gctcgcaacg ttctaccagg cgtaaacctg
     8521 aaaaccagcc cacgcgtgtg atcctgttca ataaacccta cgatgttctt ccgcagttca
     8581 ccgatgaagc cggacgcaaa acattaaaag aattcatccc ggttcagggc gtttatgcag
     8641 caggtcgtct tgaccgcgat agcgaagggt tactggtgct gaccaataac ggcgcgttgc
     8701 aggcgcgttt aacccagccg ggtaaacgca ccgggaaaat ctattatgtg caggtggaag
     8761 gtattcccac acaagacgca cttgaagcct tgcgcaatgg cgtaacctta aatgatggcc
     8821 ctaccctgcc cgccggcgcg gagctggttg acgaaccagc gtggttatgg ccgcggaatc
     8881 caccaattcg tgaacgcaaa agtattccca ccagctggct gaagatcacc ttatatgaag
     8941 gacgtaatcg ccaggtgcgc cgcatgaccg cccatgttgg tttccccacg ctgcgactga
     9001 ttcgctatgc gatgggagat tactctttgg ataatcttgc caatggcgaa tggcgagaag
     9061 tgacagatta aggatgcaaa atgtttaaac cgcacgttac cgttgcttgc gtggtgcacg
     9121 cagaaggcaa atttttagtc gttgaagaga cgattaatgg taaagcgtta tggaaccaac
     9181 ctgccgggca tctggaagcc gatgaaacct tagtggaagc cgccgcccgt gaactgtggg
     9241 aagaaaccgg catcagcgcg cagccgcaac actttattcg tatgcatcag tggattgcgc
     9301 cagataaaac gccgtttttg cgtttcctct ttgccattga gcttgagcaa atatgcccga
     9361 cccagcctca tgacagcgat atcgactgct gtcgttgggt cagcgccgaa gagattttaa
     9421 aggcgtcaaa tcttcgttcg ccgctggtgg cggaaagtat ccgttgttat caaagcgggc
     9481 aacgctatcc gctggagatg attggcgatt ttaactggcc ttttacaaag ggtgtcatct
     9541 aaaaggctgc gtgatagaat acgccgcctt gaagttcaat gtcgtgagtg atccaatgtc
     9601 tgaaaccgca aaaaaagtaa tcgtcggcat gtccggcggt gtcgattcct ccgtttctgc
     9661 ctggctgttg caacaacagg gatatcaggt tgaaggcctg tttatgaaga actgggaaga
     9721 agacgacggt gaggaatatt gcacagcggc agcggatctg gctgatgccc aggctgtctg
     9781 cgacaagctc ggcattgaac tgcacaccgt taactttgct gccgagtact gggacaacgt
     9841 cttcgaactg ttccttgccg aatataaagc cggtcgcacg ccgaatccgg atattctgtg
     9901 caacaaagag atcaaattta aagccttcct cgaatttgcc gccgaagatt taggtgccga
     9961 ttatatcgct accggtcatt acgtacgtcg ggccgatgtc gatggcaaga gccgcctgct
    10021 gcgtggtctg gacagcaata aagaccagag ctacttcctt tatacgctca gccatgagca
    10081 gattgcgcaa agcctgttcc cggtcggcga actggaaaaa ccgcaggtgc gtaagattgc
    10141 tgaagatctt ggtctggtca ccgcgaagaa aaaagactct accggcattt gcttcattgg
    10201 cgaacgt
//
LOCUS       DAEVZA010000159        10106 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEVZA010000159 DAEVZA010000000
VERSION     DAEVZA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.159"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            218..532
                     /locus_tag="LAF92_004330"
     CDS             218..532
                     /locus_tag="LAF92_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793248.1"
                     /translation="MGKPTFRSFYDVVRELEDVYGHKELWLYSGAAYATPTEMINARH
                     NWKSPKILKRNGRMVAERMDNSDSWQLVGDYKKPLFQHCAPPWQSCQIDDYFKGYYII
                     AP"
     gene            585..1364
                     /locus_tag="LAF92_004331"
     CDS             585..1364
                     /locus_tag="LAF92_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793249.1"
                     /translation="MGSMKELLFEMQEERRDEWIAENYPDAEEGTPEWDAAAQEYSWF
                     QDWMEEAAEQQYFEASLASIPDRLQDAKAELDELESLMQFNQPRIVERMAYVHCVSVL
                     DSFLMYSARALLSHPPHLQKFLHEADSLVPNKEDRRKLLASKWVEQEPDKDTPEKVYT
                     WRAQSLVAKKTFQSHKVIGWYFSRMLTTPHEWPLEEIKGVIKIRNALVHRNGVTESLE
                     PVYISSGSVQNAICTVRAFITVAAETLLQEDALYRTDDGIF"
     gene            1415..2194
                     /locus_tag="LAF92_004332"
     CDS             1415..2194
                     /locus_tag="LAF92_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBI9793250.1"
                     /translation="MTSLPANCIPVSNTSLPVAIDYPAALALRQMALVQDELPKYLLA
                     PEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQRT
                     EKAARTAGRMPAGQQTHRLVPLSDAWFVSQLQTMVATLKIPMERRNKRTGRTEKARIW
                     EVTDRTVRTWIGQAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMGH
                     KSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNRHA"
     gene            complement(2332..2607)
                     /locus_tag="LAF92_004333"
     CDS             complement(2332..2607)
                     /locus_tag="LAF92_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBI9793251.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(2601..3245)
                     /locus_tag="LAF92_004334"
     CDS             complement(2601..3245)
                     /locus_tag="LAF92_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9793252.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAARAEIEHLMNEVFGPW"
     gene            3474..4445
                     /locus_tag="LAF92_004335"
     CDS             3474..4445
                     /locus_tag="LAF92_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein parM"
                     /protein_id="HBI9793253.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVMD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            4450..4842
                     /locus_tag="LAF92_004336"
     CDS             4450..4842
                     /locus_tag="LAF92_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBI9793254.1"
                     /translation="MDDERKRKKFTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGALQ
                     SAEKMPPSATDSVQEAMNDVRLKMQKLF"
     gene            complement(4847..6118)
                     /locus_tag="LAF92_004337"
     CDS             complement(4847..6118)
                     /locus_tag="LAF92_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBI9793255.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPSYTTQWTEIPIASF"
     gene            complement(6118..6555)
                     /gene="umuD"
                     /locus_tag="LAF92_004338"
     CDS             complement(6118..6555)
                     /gene="umuD"
                     /locus_tag="LAF92_004338"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBI9793256.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(6552..6800)
                     /locus_tag="LAF92_004339"
     CDS             complement(6552..6800)
                     /locus_tag="LAF92_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBI9793257.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            7218..8120
                     /locus_tag="LAF92_004340"
     CDS             7218..8120
                     /locus_tag="LAF92_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBI9793258.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIIDNVAHYGG"
     gene            8117..8429
                     /locus_tag="LAF92_004341"
                     /pseudo
     CDS             8117..8429
                     /locus_tag="LAF92_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8505..9188
                     /locus_tag="LAF92_004342"
     CDS             8505..9188
                     /locus_tag="LAF92_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBI9793259.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            9189..9410
                     /locus_tag="LAF92_004343"
     CDS             9189..9410
                     /locus_tag="LAF92_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793260.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            9424..9858
                     /locus_tag="LAF92_004344"
     CDS             9424..9858
                     /locus_tag="LAF92_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBI9793261.1"
                     /translation="MYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNNTREND"
     gene            9903..>10106
                     /locus_tag="LAF92_004345"
     CDS             9903..>10106
                     /locus_tag="LAF92_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793262.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIYVL
                     LDAPRLATLLINRHGGVLPPLLAS"
BASE COUNT         2394 a         2458 c         2670 g         2584 t
ORIGIN      
        1 cgtggtacac ttccggaaag ttgtgatatt caggaaagtc ggatctgacg aaaacggctc
       61 tccggtaatt taacggcgtg gttatgtggc tgcttattat cagccttgaa atgatgacgt
      121 catgggaaaa tcatccgtgt gacagaagca gggttgtgct gcgcgtcatc attactctgc
      181 gtgactattg aaccggatac tatttttgag gcgaggtatg ggtaaaccaa catttagatc
      241 attttacgat gtggtccggg agcttgagga tgtatatggc cataaagagc tatggctgta
      301 ttcaggagcg gcatacgcaa cgccaacaga aatgattaac gccagacata actggaaatc
      361 gccaaaaatc ctgaaacgta acggaagaat ggttgcagaa cgaatggata actcggactc
      421 atggcaactg gttggtgact acaaaaaacc gctgttccaa cattgtgctc cgccctggca
      481 atcctgtcag atagatgact attttaaagg ctattacatt atcgccccct gaaggtcaga
      541 ggagtaattg atggcgggat tacatcattg atggcgaggt ttaaatggga agtatgaaag
      601 aactgctgtt tgaaatgcag gaggaacggc gtgacgaatg gattgcggaa aattatccgg
      661 atgctgagga aggaactcct gaatgggatg cggcagcgca ggaatacagc tggtttcagg
      721 actggatgga agaggcggct gagcagcaat attttgaggc ttcgcttgcc agtattcctg
      781 accgcctgca ggatgcaaag gctgaactgg atgagttaga gagcctgatg cagttcaacc
      841 aacccaggat cgtagagcgg atggcttacg ttcattgcgt gagtgtactg gacagctttc
      901 tgatgtacag cgcaagagca cttcttagtc atcccccgca tttacagaag ttcctgcacg
      961 aggctgattc gttggttcct aataaggaag acagaagaaa attacttgct tccaaatggg
     1021 tcgagcagga gccggataaa gacactcctg agaaggttta tacctggcga gctcagtctc
     1081 ttgtagcgaa gaagacattt cagagccaca aagtgatagg ttggtatttt tcaaggatgc
     1141 tgacaacgcc acatgagtgg ccattggagg agattaaggg tgtaatcaag atccgaaacg
     1201 cactggtaca ccgtaatgga gttaccgaat ctcttgaacc cgtgtatatc agttcgggga
     1261 gtgtgcagaa tgctatctgc acggtacgag catttattac cgtggcggct gaaacccttc
     1321 tgcaggaaga tgcactatac aggacggatg atggcatatt ctgaaattac cgcagcgaca
     1381 ccatccataa gcgtcttcac tgagggatat gtcgatgact tcgcttcccg ctaactgtat
     1441 accagtaagc aacacatcat tacctgtcgc catagactac ccagcagcgc tggcacttcg
     1501 ccagatggct ctcgttcagg atgaactccc gaaatacctg ctggcgccgg aagtcagcgc
     1561 cctgctccac tacgtgccgg atcttcaccg caagatgctg ctggcgacgc tctggaacac
     1621 gggcgcgcga attaacgaag cgctggcgct gacaaggggg gatttttcgc ttgcgcctcc
     1681 gtatccgttt gtgcagcttg cgaccctgaa acaacggacg gaaaaagccg ccaggacggc
     1741 aggaagaatg cccgccggtc agcagactca ccggctggtt ccgctctccg acgcctggtt
     1801 cgtcagccag ctgcagacga tggtggccac actgaaaatc cccatggaac ggcgtaataa
     1861 acgaacaggc aggacagaga aagcgcggat ctgggaagtg acggacagaa cggtcaggac
     1921 ctggattggg caggcggttg ccgccgctgc cgctgacggt gtgacgttct ctgtcccggt
     1981 cacgccacat acgttccgcc attcctatgc gatgcacatg ctgtatgccg gtataccgct
     2041 gaaggtcctg cagagcctga tggggcataa gtccatcagc tcaacagagg tctacacgaa
     2101 ggtgtttgcg ctggatgtgg ctgcacggca ccgggtgcag ttttcgatgc ctgagtccga
     2161 tgctgtcaca atgctgaaaa acagacacgc ataagtcaca aatcataatt atgaattgtg
     2221 atttattcta taaaagaaga gaccactgca atatgtgatc tcttgtatgc aagggtgctt
     2281 aaacagtgtg aattcacaag tgtgatttca taagtaataa cttcttgatt attacgttag
     2341 tgtttttaag tattgctcaa gggctactct gacgatggca cttacatttt ttggatcttt
     2401 accattgcgt ttattcctca atgccagatc ttctattgtg acaagcatgc tttcaccaag
     2461 agagattgtc gtccgacatt gtttttctgg ttcgggtttt tccggcgcgc cgtatggttt
     2521 atccgcaagg cgctgagcca gagcctctgc ttgttctacc gtaactgcag ctctgtttaa
     2581 tgcttgctgg ctgggtttct tcaccatggg ccaaatacct cattcattaa atgttcaatt
     2641 tctgctctgg ctgctgtatt gttcgtttct actacaccgg tgccgttgct catgcaatcg
     2701 cgatagactt ttctgaaaca aataactgaa tccaggactt gaattgttgg gaattcttca
     2761 agatattcaa ggaattcttt tctctcattt cccctcaaaa ccggattggt tgttgccata
     2821 ctttgtaaac aataaacctt aagttccggg ttaaggttac gcattgcgtc tatctgttgt
     2881 tctagttctg taagagtatc cagatctggt tgagaacact gtagtggcgc aattatttga
     2941 tgtgcgacaa ctccactcgt tatgaattcc tttgagtttc ttcctgcgac atccactatt
     3001 acatcatcga acttttcatt cagagcctgt agtgtttgtg tcaggttatc aaatttctca
     3061 acaagggtta ttgctggtaa cagtcctgca gcttctcgtt ctgcatgatg ttttgcggct
     3121 gttcgttgta gatctccgtt taacaggcag acttcccttc ctttatttgc gagagcaata
     3181 gccaggtttg atgcaatggt agttttccct gaaccacctt tgttcccacc aactacagta
     3241 atcattgcaa cctcataaat gtgatgtgtg aagtatgatg atattttgac acggtaacct
     3301 gatgtgtcaa catgtattca tatttgtgaa gtatgaaata tgaagtatgt catatgatgt
     3361 aataaatatt acttgtgttt tgtgatttca taactatgat agtgctcaaa ttgagtatta
     3421 ccttgcaacc agaaacagtg atttactcag attgagtatt taaggggcta tagatgaacg
     3481 tatactgcga tgatggttca acaacaatca agttggcatg gaatgacaat gggaaaatct
     3541 gtaagtcgtt gtcgcaaaac tcatttcgtc atggatggaa ggttgacggg ttagggattc
     3601 gccagacatt taactatgaa ctggatggta aaaaatacac gtatgatgaa gttagcaacc
     3661 aatctattct tacgactcat attgagtatc aatatacgga tgttaacctg ttagctgtgc
     3721 atcacgcttt actgaatagt ggattagcgc ctcagcctgt atcattgact gtgactttgc
     3781 ctatcagtga gttctacacg aaagagtgtc aaaaaaatga actcaacatt cagcgtaaaa
     3841 ttgagaattt aatgaggccg attcgtctta ataaaggcga tgttttcacc attgaacacg
     3901 ttgatgtgat gcctgaatca ttgccagctg tgttttctcg tctggtcatg gataaagttg
     3961 gtcagttcga aaaatcgctg gtagttgata ttggcggcac tacgcttgat gttggtgtta
     4021 tcgttggtca atttgactct gtaagtgcca ttcatggtaa ttctggcatt ggtgtgtctt
     4081 cagttacgaa agctgcaatg agtgcattgc gcatggcatc cagtgatacc agtttcctcg
     4141 ttgcagatga attgattaaa cgtcgtaatg acccggactt tgttcggcag gttataaatg
     4201 acgaaacaaa aactgatctg gtccttaata ccattgaggg ggctattgcc agtctggggg
     4261 agcaagtagt caatgagctc ggtgattttc atcatgtgaa tcgtgtttat gttgtcggtg
     4321 gtggggcccc gttgatctat gattcgataa aaacggcatg gcaccatctt gggcaaaaag
     4381 tagtgatgat ggagtcgcca cagacagcac tggttgaagc tatcgcggct ttcaaagagg
     4441 agtaatattg tggacgatga acggaaaagg aaaaaattca cactttatct gcatccggaa
     4501 aaagctgcag actttcaaac tttggaagcg atagagtccg ttccgcgttc tgagcgtggt
     4561 gagctgttcc ggaatgcatt tatttctgga atggcgttac accaacttga tcctcgcctg
     4621 cctgtattgc tgactgccat tttgagcgag gagttttcag cagatcaggt tgtaacctta
     4681 ctcagtcaga cgacaggatg gaagccttct caggccgata tcagagctgt gttgactgaa
     4741 ctcggtgctt tgcaatctgc tgaaaaaatg cctccttctg ctactgattc ggtgcaggag
     4801 gcaatgaatg atgtgcgcct taaaatgcaa aaattattct gatatatcag aatgaagcaa
     4861 tggggatctc agtccattgt gttgtatacg aaggcgagag catttcccgt ttcatttgcc
     4921 attcaggggc ggttcctcgc cccgcaaacc acactttccc ttttccagac tggttcagtt
     4981 catcgagtgt tttcattaac ttttcgctgt ttttacgggg ctggatctca tcaaataatc
     5041 ccggctgcgc tatacccgat ggtgtgaaat cagccagcat gactcctgcc ttcatatagc
     5101 ggtacccttc acgccagaca tggtttaacg ctctgcatgc tgcggcaata atgtcccggc
     5161 tgtcctgtgt gggtaatgga agcttttcca cagcggcatt gctgtaacag ggttctttta
     5221 ctgcaaaggg ggatgtccgt acaaatgtcg tcacctgccg gcaatactga cgctccccac
     5281 gtagtttctc tgcggcccgc tctgcatact gaacaacagc ctggtgcatg gcatctttgt
     5341 ctgtgattcg ttcaccaaaa ctgcgactac agacaatctg ctgttttgcc ggtggtgctt
     5401 cttccaggga tatgcaggac tcgccgttga gttcgcgtac cgtacgctca agaatgacgc
     5461 tgaagttttt ccggatgaat gccgtgttag cctgcgccag ctgcagtgct gtgttaatac
     5521 ccagcgcatt cagcttttcc gtcagtctgc gtcctactcc ccagacctca ccaactggct
     5581 gcagtcccag tagcttcaag atccgattac ggttttctgc cgtcagcgcg accacaccgg
     5641 aaaactgtgg ccattgcttt gttgcccact gtgcactttt tgccagcgtt tttgtaggcg
     5701 caatgcccac ccccatggtg agtcctgtcc agctctttac ctgttccctg agctgatgac
     5761 caaaaaactc cggagagatg caatgattta tcccccgcaa atcaataaac atttcatcaa
     5821 ttgagtaggg ctcaactgcg ggagaaagcg actccagaac agccatgacc cgttggctca
     5881 tgctgtggta cagcgcataa ttgctggaaa atacatgtat tttcttctcc aggcgcatct
     5941 gtctcacctg aaaccagggc tgccccattc tgatgccaag agcttttgcc tccgggctgc
     6001 gcgcgatcac acagccatcg ttattgctga gtacgatgac cggttcgttg cgaaggtccg
     6061 ggcggaaaac tttttcacat gaggcgtaga aactgttgat atcagccagt gcaaacatca
     6121 gcgtaactcc cgcgtcctgt gtatcacgtg agtgacaaca ccaaaaatac agatgttttc
     6181 cggatacagt gtgcggaact ccgggctgtc tgaaaccggc tccagtgccg ggcgtgggcg
     6241 caacagcagt cgtttgacgg tgaactcacc gtcgatctca gcgataacga tgtccccgtg
     6301 ttgtggtttt tcggccctgt ccactaccag cagatcacca ttctgcacgc cagcctggtt
     6361 catcgattca ccgctggcgc gcagaaagaa ggtggctgca ggtctgctga tgcaatagct
     6421 gttcagatcc agttcctgct cagcataatc agtggcaggc gaaggaaaac cggcctggca
     6481 acgatcggca aacaacggtc gcacgtaact gtcatcgcct gaagggtcag ccggacggtg
     6541 ataaacggta ctcatgctgc aggcagccag ctgtcgtcct gccagatttc ttccagcaga
     6601 gacaggatac gttctttatc cttgtcgttg cgggctcctg tgacagatac agacggggcg
     6661 ctgccctggt ctatacgcag ccagatttcc gggtagtgtg gtttcagacg ttgttcgatt
     6721 tcattctgta gcgcctgaag tgtgcctgat ttgaggtttt ttgtgctctg gcggtcgaaa
     6781 agaatttcaa tgcggatcat gttctcatct cccttttcta ttgctgtatg catatacagt
     6841 attctaataa ctgaatccac acacagtcaa atgtcattat tgtgactaaa aaacgctcaa
     6901 ttctctgagt gtgtggctgt atgcctgctg tgggctgctg tgtccagctt atccacagca
     6961 ttttgcgcac ggttctgtga ataaaatacc tggttaccca ggccgtgccg gcacgttaac
     7021 cgggctgcat ccgatgcaag tgtgtcgctg tcgccggcct cctcacccgg tcacgtttcg
     7081 tcgttcctcc tccacgcgct gcggcttcgg ggccgcacct gcattcgtat gcggtcgccc
     7141 ggttacaggt gcggcacggc ctgatggagg ccgcatgtga gaggagaatt cccatgccaa
     7201 actggtgctc gaatcgtatg tatttttctg gtgaaccggc acagatcgct gagattaaac
     7261 gactggccag cggtgcagtt acaccgcttt atcgccgcgc cacaaatgaa ggtattcagc
     7321 tgtttctggc cggaagtgcc ggacttctgc agatcactga aaatatccgc tctgagcagt
     7381 gccccggagt gacggctgcc ggacgtggtg ctgtatcacc ggaaaatatc gcgttcaccc
     7441 gctggctgac acacctgcag aacggtgtac tgctggatga gcaaaactgc ctgatgctgc
     7501 atgaactctg gctgcagagc ggtactggac agcgtcgctg ggaagagtta ccggatgatg
     7561 tcagggaaac tatcactgtg catttcaccg caaaaagagg tgactggtgt gacatctggg
     7621 gtaacgagga tgtatcggtg tggtggaacc gtctgtgtga caacgtactg ccggaaaaaa
     7681 ccatgccgtt tgacctgctg acggttttgc cgacccgcct ggatattgaa gtgaatggct
     7741 ttaacggcgg tgttctgaac ggtgttcctt ctgcatatca ctggtataca gaacggtatg
     7801 gcgtgaagtg gccttgtggg tatgacctga atatcagcag tcagggggaa aacttcattc
     7861 aggtagattt cgacacgccg tggtgtcagc cggaaagcga cgttgttgca gaattaagcc
     7921 gccgtttcgg ctgcacgctg gagcactggt acgccgaaca gggctgcaat ttctgtggct
     7981 ggcagttgta tgagcgcgga gagctcgttg atgtgctgtg gggggaactg gaatggtctt
     8041 ccccgacaga tgatgatgag ctgccggaag tcaccggacc tgcgtggata atcgacaatg
     8101 tggcgcatta tggcggatga agtatgacgg agacgggcgg gcaaccgccc gtttcttttc
     8161 cgacaaagga tgtcgccgtg ctcctctttt tactgcgccg gctgacgcgg cgcggcataa
     8221 acgctgcctg tggtcagtgt cccgcgtccg gccgggcacg ggacgggcgc ttttaccgct
     8281 cccggctggc gtcggtgaca gtgtacgcca gcccgtcgcc cttttctgat gaacgccctt
     8341 caagccgctt tcgcggcata gccttgccgt caaaaagacg acggctgcgg tattccacgg
     8401 tcggcctgac ccgttaccgg acgcggtgaa cagcccacag gcagcgggga acgggcacca
     8461 caggggtgcc ctcccggtgc cctctgtaag agaaggagtt tggtatgtcc cgttttgtcc
     8521 tcggtaactg catcgatgtt atggcccgta tccctgataa cgccattgat ttcatcctca
     8581 ccgacccgcc gtacctcgtc ggttttcgtg accgtcaggg gcgtaccatt gccggcgata
     8641 aaaccgatga atggctgcaa ccggcctgta atgagatgta ccgcgtactc aaaaaagacg
     8701 cgttaatggt gagcttctat ggctggaatc gcgtcgatcg ttttatgtct gcctggaaaa
     8761 atgcgggatt cagcgttgtt ggtcacctgg tcttcaccaa aaactacaca tcgaaggccg
     8821 catatgtggg ctatcgccac gaatgcgcct acatcctggc aaaaggccgt ccacgtctgc
     8881 cacaaaaccc gctgccggac gtgctgggct ggaaatattc gggcaatcgc catcacccga
     8941 cggaaaagcc cgttaccagc ctgcaaccac tgattgagag cttcacacat ccgaacgcga
     9001 ttgtgctgga cccgtttgca ggcagcggat caacctgcgt ggccgctctt cagtccggac
     9061 gccggtatat cggtatcgag ctgcttgaac agtatcaccg tgccggacag caacgactga
     9121 ccgccgtgca acgggccatg cagcaggggg ccgcgaatga taactggttt gaaccggagg
     9181 ctgcgtaaat gaactatgcc ggacacgaaa aacttcgcgc cgacgtggcg gaggtggcca
     9241 acaccatgtg tgaccttcgg gccaggctga acgatatgga gcatcgctgt cgctttgatt
     9301 ccgatgtgct tgttgaacgc ctgacacgcc agacgctgta tcgcgccaat cgcctgttca
     9361 tggaagcgta taccgaaatt ctggaactgg attcctgctt caaagattga gaaggagacg
     9421 agaatgtacg gaacatgcga aacgctctgc cgggcgctgg cagcaaagta tccgggagat
     9481 acgccgctga tgctggttat ctggtccccg gaagaaattc aggccctcgc tgacggaatg
     9541 gatatttccc tgtccgatca tgaaatcagg actgtcctgg cgcgcctgga ggacatcccg
     9601 gaagaccagc ggactgaatc cggtatttct tccggtgcgg cgatggagat catcaataac
     9661 gtgagagaaa accgccaggt gaccgtccct gctgaactgc tggcgtccct gattcagacc
     9721 gccgaacagg cactgtggaa acgtgagtgg gccgcccgtg ataacggcct cgccgtcccg
     9781 gaatgcgtca cccgccgaca ggcggtggtt aatcaggccc gcgccctgct gaaaaacaac
     9841 acacgcgaaa acgactgatg ttatcgccgc ctgcgggcgg cgattcaggg agaccatgaa
     9901 tcatgaacga aacgctgaat gcactgattt gtcgccacgc ccgtaacctg ctgctggcgc
     9961 agggctggcc ggaagagacg gatgttgacc agcgtaaccc gaagtatccg ggctggatca
    10021 gcatttatgt tctgctggat gcgccccggc tggcgacgtt acttatcaac cgtcacggcg
    10081 gcgtactgcc gccgctcctg gcctct
//
LOCUS       DAEVZA010000160         9958 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEVZA010000160 DAEVZA010000000
VERSION     DAEVZA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.160"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..237
                     /locus_tag="LAF92_004346"
     CDS             <1..237
                     /locus_tag="LAF92_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI9793263.1"
                     /translation="KTTRTPIPVTNSTQDSQLHNGLKKTLHDALTAKIQLTSFEAKFL
                     SDMQSKHDLNDSFTWLTQKQRATLEKILAKYGRF"
     gene            245..694
                     /locus_tag="LAF92_004347"
     CDS             245..694
                     /locus_tag="LAF92_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBI9793264.1"
                     /translation="MALVSISEAARLTGKSRTTVQSYIKQGKLTKCTDSSGSSKLDTS
                     ELLRVFGSFTGQQLGSEQSGSTVQSLAGESVQLTDQNLLHENYALKAEIELVKALLRE
                     KELLLAEKDKRNEDLKQALLLIESKLPATPEPVTQPMVKKSWKFWKK"
     gene            673..822
                     /locus_tag="LAF92_004348"
     CDS             673..822
                     /locus_tag="LAF92_004348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793265.1"
                     /translation="MEILEKIEVSARLLAEIISVVLITNRQHKNSQFWGQKTGCFARF
                     DVFSS"
     gene            1342..1881
                     /locus_tag="LAF92_004349"
     CDS             1342..1881
                     /locus_tag="LAF92_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793266.1"
                     /translation="MKRLLAASLALALAGCATPAPKAPHPDKAAIEAAEIARYSDQQL
                     CQASDAIQPSLNVNPSSHPEYNDNLSRSKALEAAARSRNLSCYKLKAANSKAIKPMTA
                     RQKQARATAVCEGYAQRTQFANPSVAYSMCLKGAQSTPEKCTRDNTHFSSEATKLTGA
                     SRAEYTEIGNAFRIGCNLN"
     gene            1892..2341
                     /locus_tag="LAF92_004350"
     CDS             1892..2341
                     /locus_tag="LAF92_004350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793267.1"
                     /translation="MKLRHTLIMSAAFLMAYQANAGTPSTIDPAAKEIASMMCRWVLD
                     GGKLTDPAQRLTIYENGKYERWLELHPSDKGGMPPIVYERHREGGFVADDKYKEYYWK
                     GSNFRLTMLRDAILRKSEVTLALEDIYNASMPISDAKCENVTYANSN"
     gene            2325..2867
                     /locus_tag="LAF92_004351"
     CDS             2325..2867
                     /locus_tag="LAF92_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793268.1"
                     /translation="MQTATNSSVKFQPWVGDHYHSSRYGVRVMVLGESHYGEPEDYAP
                     DFTQHVINEHAFQPGLRFFTIATNLLRGTTDEPTAEERREAWQHVAFYNYVQEFVGDA
                     GRIRPTRAMWRDAATVLEEVVAELRPDVILVLGYQMWDHLPELPVTWACVKHPCGGMS
                     YDEAIPEFNRAIAEALSLAG"
     gene            2939..3346
                     /locus_tag="LAF92_004352"
     CDS             2939..3346
                     /locus_tag="LAF92_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010670463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793269.1"
                     /translation="MSIQRREVYLVLKKLPDGRVIYVEGIRCAHNYFFDGHSRVGLSA
                     GSSASFVEFSPNERPMQLFHMPNENNGKWQAAFHNFGCSPEFGEPEMEYIIFVGAYSN
                     DRDCTFDLMREGLNRAWEIVEGNRQLRDGEFND"
     gene            3389..3592
                     /locus_tag="LAF92_004353"
     CDS             3389..3592
                     /locus_tag="LAF92_004353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793270.1"
                     /translation="MALALHHVFEILQFSASKPSTAREVRGIVTHAYKSSLRQFTLFS
                     GLVSLDALSHIEDGTYKGRTLLR"
     gene            3841..5889
                     /locus_tag="LAF92_004354"
     CDS             3841..5889
                     /locus_tag="LAF92_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBI9793271.1"
                     /translation="MQTELIKELNKVLKAFPQFWNGEELQRSMVSDAISQKQPDLIKA
                     LVANEKIRAVYGTDIDGIFIFDFDNLCKFLKYKEYWANSFTKYRNKVGLTSEGKYLDY
                     SSDVVLDFPFKDCVLEGGMTKENQGKDEIYYNEIIARDEIDRLFSPKVFTNVKRHTKN
                     GAQNNISYFDQSDNLIIKGNNLIALNSLITPHKGEVKLIYIDPPYNTGSDSFKYNDRF
                     NHSTWLTFMKNRLETSLKLLSDDGFIFIQCDDNELFHLKVLMDNIFGVENFINIITIK
                     TKIGGVSGSSEGKSLRDATEFIILYSKRKELAMLNPVFKKTEVIQYVNEYKDSGKSWK
                     YTQILLDLGAKTPIKSEDGVTYNHYPYAKTTSISRFSKEKGISENDVYKDYAGIVYRT
                     TNAQSSVRGRVLKELEEINNGIVSVEYTPLKGKNKGTPVEIFYNASNKDMFMFLSDML
                     EKNGESYNYMQKINTLWDDIQYNNLNKEGGYVSFENGKKPELLIKRVIELSTKAGDLI
                     LDFHLGSGTTAAVAHKMGRRYIGIEQMDYINDITVPRLQKVIGGEQGGISKDINWQGG
                     GSFVYAELMELNAYFVHEIQKAQSTEELEKLFAVMKTEAHLNYQVALENVLSAEYEVD
                     GIFRKVAFSELELHEQKQLLIEILDKNQLYVNASDMDDSDLNISESDKAFTRSFYGME
                     "
     gene            5894..8530
                     /locus_tag="LAF92_004355"
     CDS             5894..8530
                     /locus_tag="LAF92_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBI9793272.1"
                     /translation="MAKKPTSAGSEKLLFNKLKQFDEPGLFHDNYQTPDYIQANLKDA
                     LRPYQHGALRYLHYTQRKREEALLHYRHLLFHMATGAGKTMVMAGTILYLFKEYGYQN
                     FIFFVHTDAIIQKTRENLLNPQSPKYLFSQELEIDGEKITIAPVETFPSVPERNTIYL
                     KLSTIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKARGKSKTSPENEE
                     QTWERTIENILDLRPDNRLFEFTATIDLANKDIGQKYRDKVVYQYDLKQFMSDGYSKK
                     VMLLEANQNDGDKMLDAVLLSQYRKLTAADNGITGFKPVILFKSNKIAISKAKQEEFS
                     QLIAAMTPESVRRHLANKRVQLSSDTSIWHKVIQRYADSDLVTVIQQIQEDFNDFNLL
                     NVNKSDLLEENPVLLNTLENIDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKT
                     GTDSEAQLIGRGARYYPFVYDGKRSFTRRFDNSAKDLSVLEQLHYHTINEPAYIKTLH
                     ASLEQADIDVHQDGSGTIEHARLKEDFKKSTVYQTGKLYFNKVEEIESSSRRWETYSL
                     ETRFEIPYQTAGEESLDNLTGATAVITKPEPLVLDERFYRKAMQRISFYALDNLQRFF
                     PKLTGIREFIRSDAYLGKLKITVTVPQSLDFSSVPAKEKLHLLETVLLRISENIRRND
                     QKVKGTYRFISQPVKEVIKDYSLHIDPSVVINQKITTAPTIGKKWYVYDNAILNQLEH
                     RLVKTLEAFMPKLKARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTRHSDNT
                     YWQVFLEPKGDDRLLDDAWKEQMLETLNDRERIVIDENEDVRLVGIKFFANSQMDAFV
                     SDMQNRLNEGESLETASFSLPL"
     gene            complement(8573..9157)
                     /locus_tag="LAF92_004356"
     CDS             complement(8573..9157)
                     /locus_tag="LAF92_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="HBI9793273.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVSTLTEEFQAASVQLQRQSETLS
                     KVIRELDSASGNMTDTVRKSVSSAMNQVTQALTQAGTEQQKPAVNALNQVVKAANDSV
                     NAMRREMYRYTWKSAIYLVLTIFFVLACCVTALTWFMNDGYSRIAEMQRMEAVWQKKA
                     PLADISTCDGKPCVKVDTRTTYGDKENTWMIIKK"
     gene            complement(9170..>9958)
                     /locus_tag="LAF92_004357"
     CDS             complement(9170..>9958)
                     /locus_tag="LAF92_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBI9793274.1"
                     /translation="HNPTAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKVPE
                     RGGAKKVRFGETPTERKEHLIAQRERWADLQNAYLERYQHADRVDARSLKAQGIGREP
                     ERHLGAGQVQRFDTDQLQAILERREAERQVQQCCDERDSVIDVTTSLREAISERDTLM
                     LKQTQKSDPEQDAVSGRVFDFEKEPEKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQE
                     IHQEMERQKERARLAEKQRQQEKERQRIAEQKRQKPDKGWSFSR"
BASE COUNT         2799 a         2405 c         2411 g         2343 t
ORIGIN      
        1 aaaacaacaa gaacgcccat tccggtgaca aacagcacgc aagacagtca attgcataac
       61 ggtcttaaaa agacactaca cgatgcactg accgcaaaaa tccagctcac tagctttgag
      121 gctaaatttc taagcgatat gcaaagcaag cacgatctca acgactcatt tacatggctg
      181 acccaaaaac aacgagccac gctagagaaa attttagcta aatacggacg gttctgaggt
      241 atctatggca ctggtatcaa tcagcgaagc cgcaagactg acaggaaaaa gtcgcacaac
      301 agtgcagtca tacattaagc agggaaaact gaccaagtgc acagatagca gtggttcttc
      361 aaagctagat acatcagagc ttctgcgtgt tttcggatca tttactggtc agcaacttgg
      421 cagtgaacaa tcaggcagca ctgtgcagag tttggcagga gaatccgtac agttaactga
      481 ccagaatttg ctgcatgaaa actatgcttt aaaagctgaa atagaactcg taaaggcatt
      541 gctgcgtgaa aaagaactcc tactcgctga aaaagacaag cgcaacgagg atctcaagca
      601 agccctgtta ctgatagagt ccaagctacc ggcaacacca gaacctgtca cgcagccgat
      661 ggtgaaaaaa tcatggaaat tctggaaaaa atagaggttt cagcccgttt gctggctgaa
      721 atcatatccg tcgttttgat aacaaaccgg caacacaaaa acagccaatt ttgggggcag
      781 aaaacaggct gttttgcgcg ttttgacgtg ttttcgtcgt gagtacctga tcggtggtgc
      841 gcgtaagatc ccttatgtta aataccacct gttttggtgc atttttccgc aattgtatca
      901 gcagcattac tgtcaggttc cagcattgga cgcgatacct tacaatcatt tcaatctcta
      961 acagtcataa ggccatgaca tgcagaccga attaatcaaa gaactgaaca aggttttaaa
     1021 agcgtttccg caattttgga gcggagaaga tttaaggggc cgtttgggaa acggacaata
     1081 ttgtacggta agcctgtttt ttgaacagtc gaaatcatcg cagtagggtg aactcccgcg
     1141 ctatacgacg ttttccgcgg tttttggcta atcggataac ctaaatgcga tactgattag
     1201 gcgataattt aaaagcgata gccactggct ctaaatcaat ccgttacggg tattagtggt
     1261 taaataccgc gctgaacgta caataatttc cgatctctaa actgatccct tctacaactt
     1321 gcatacaggg cactcgaacc catgaaacga ctattagccg cctcattggc gctggcatta
     1381 gcaggatgcg ctactcccgc acccaaagcc ccgcatccag acaaagccgc gatagaggca
     1441 gcggagattg ccagatacag cgatcagcaa ctttgccagg catctgacgc gatacagccg
     1501 agcctgaatg ttaacccatc cagccatcct gaatacaacg acaatcttag ccgctcaaaa
     1561 gccctagagg cagctgcccg ctcgcgtaat ctgagctgct ataagctgaa ggctgccaat
     1621 agtaaggcaa tcaaaccgat gaccgcgcgc cagaagcagg cccgtgcaac tgctgtgtgc
     1681 gaaggctacg cccagcgtac ccagttcgct aatccgtctg ttgcgtactc tatgtgcctc
     1741 aaaggcgcgc aatccacgcc agaaaaatgc acccgcgaca atacgcactt cagcagtgaa
     1801 gccactaagc tcacgggcgc aagccgtgct gaatacactg aaatcggcaa tgccttccgc
     1861 attggatgca atctgaacta agggatctct catgaaacta cgccacacac tcattatgtc
     1921 cgcggcgttt cttatggcct accaggctaa tgctggcacc ccatcaacca tagatcccgc
     1981 agcaaaagaa atagcgtcaa tgatgtgccg gtgggtgctg gacgggggta agttgaccga
     2041 cccagcgcag cggctgacaa tctacgagaa cggaaaatat gagcgttggc tggagcttca
     2101 cccgtccgac aaagggggca tgccacctat cgtgtatgaa cgccatcgag aaggcggttt
     2161 cgtcgcagat gacaagtaca aagagtatta ctggaaaggc tctaacttcc gattgacgat
     2221 gctgcgggac gccatcttgc gcaaaagcga ggtaacgttg gcacttgagg atatttataa
     2281 tgcgtccatg cccatcagtg atgcaaagtg tgagaatgta acgtatgcaa acagcaacta
     2341 attccagcgt gaaatttcag ccttgggtgg gtgaccatta tcactcaagc cgctacggcg
     2401 tccgcgttat ggtgttgggt gagtcccact atggtgaacc ggaagactac gcccctgact
     2461 ttacccagca cgttattaac gagcatgctt tccagccagg gcttcgtttt ttcacgatag
     2521 ccaccaatct gctacgaggc acaactgacg agccgaccgc agaggaacgg cgtgaagcct
     2581 ggcagcatgt agccttctac aactacgttc aggagtttgt gggcgatgct ggccgtatac
     2641 gtcccacgcg tgctatgtgg cgtgatgcag ctactgtgct tgaagaagtg gtggccgagc
     2701 tgaggcccga tgtcattttg gtgttgggct atcagatgtg ggatcactta ccggaactgc
     2761 cggtaacatg ggcttgcgtc aagcatccct gcggcggaat gtcttacgat gaggcgatcc
     2821 ccgagttcaa ccgagccatc gcagaagccc tttccctggc tggctaaagt tctttggcct
     2881 tggcaccggc tggggtcatt cttactctcc actgctaccc ataactacag gaataaaaat
     2941 gagtattcag cgtcgtgaag tgtacctggt cctgaaaaag ctgccagatg ggcgggtgat
     3001 ctatgtggaa ggtatcagat gtgcccataa ttactttttt gatgggcatt ccagagtagg
     3061 cttatccgca ggtagttcag cgagctttgt agaattctca cctaatgagc gtccaatgca
     3121 gcttttccac atgccgaatg agaacaatgg caaatggcag gcggctttcc ataactttgg
     3181 ctgctcgcct gagttcggcg aacctgaaat ggaatacatc atttttgtcg gtgcctattc
     3241 taacgaccgc gactgtactt tcgatctgat gcgtgaaggg ttaaatcgtg cgtgggaaat
     3301 tgttgaaggc aatcgccaac tgcgagatgg cgagtttaac gactaacgac actgctcatg
     3361 ctatggggct ttattttaag gacgccccat ggcactagcc ctacaccacg tctttgagat
     3421 cctgcaattt tcggcctcaa aaccgagcac cgcgcgtgaa gtgcgcggta tagttaccca
     3481 cgcatacaaa agctcattgc ggcagttcac gctgttctcg ggcctggtat cgctggacgc
     3541 tctttctcac atcgaagacg gcacctacaa agggcgtacc ctactgcgat gatttggcct
     3601 gttcaaaaaa caggcttgcc gtacaatatt gtccgtttcc caaacggacc ccttttacgt
     3661 gttccggcgt gttttcgtgg tgagtggtgt tcgggtggtg cgcgtaagag agtttatgtt
     3721 aaataccact tattctggtg cattttcccg caactgaatt caactcatcg ctgtcgggtt
     3781 ccagcgccgg acgctatatc ttacaatcat tcagatatta aacagtcata aggtcatgac
     3841 atgcagacag aattaataaa agaacttaac aaggttttaa aagcattccc gcagttctgg
     3901 aacggagagg aattacaacg ttcaatggtg agtgacgcta tcagtcaaaa gcagccagac
     3961 cttatcaagg cattggtggc gaatgaaaaa atccgcgctg tttacggtac ggatatagac
     4021 ggaattttta tttttgattt tgataattta tgcaaatttc ttaaatacaa agaatactgg
     4081 gcgaacagct tcactaaata ccggaacaag gtgggattaa ccagtgaagg gaaatacctt
     4141 gattacagct ctgatgtggt gctggacttt ccttttaagg attgtgttct tgaaggcgga
     4201 atgaccaaag aaaatcaggg gaaagatgag atttattaca atgaaatcat tgctcgtgat
     4261 gagattgatc gacttttctc tccaaaggta ttcactaatg ttaaacgaca tacaaaaaat
     4321 ggtgcacaaa acaatatatc ttactttgat caaagtgata atttaatcat caaaggaaat
     4381 aacttaattg cccttaattc attaataacc ccccataaag gcgaggtaaa gttaatatat
     4441 attgaccctc catataacac tggttccgat agctttaaat ataatgatag atttaaccac
     4501 tccacgtggt taacattcat gaaaaatagg cttgaaacat cacttaaatt attatcagat
     4561 gatgggttta tttttataca atgtgatgat aacgagttat tccatttgaa ggtgttaatg
     4621 gataacatat ttggtgtaga aaacttcatc aatattataa caataaaaac aaagataggt
     4681 ggtgtatctg gaagctcgga aggaaaaagc ttaagagatg caactgaatt tataatttta
     4741 tattcgaaaa gaaaagaact tgccatgctt aaccctgttt tcaaaaaaac agaggtaatt
     4801 cagtatgtaa atgaatacaa ggatagcggt aagagctgga aatacactca aatactctta
     4861 gatttaggtg caaaaacgcc cataaaaagt gaagatggag ttacttacaa tcactacccc
     4921 tatgccaaaa ccacctccat atctagattt tcaaaagaaa aaggcataag tgaaaacgac
     4981 gtatataaag actatgcagg gattgtttat agaacaacaa atgcacaatc cagtgtcaga
     5041 ggccgagttc taaaagaact agaagaaata aataatggca ttgttagcgt agagtacaca
     5101 ccattaaaag ggaaaaacaa aggaacgcca gttgaaatat tctacaacgc cagcaataaa
     5161 gatatgttta tgttcctatc cgacatgctg gagaaaaatg gtgaaagtta taactacatg
     5221 cagaaaataa ataccttatg ggatgacatc caatataaca atttaaataa agaaggtgga
     5281 tatgtaagtt tcgaaaacgg aaagaaacca gagttgttaa ttaaaagggt catagagcta
     5341 tctacaaagg caggcgattt aatcttagac tttcatttag gtagcggaac aaccgcagca
     5401 gtagcccata agatggggcg tcgctacatt ggtattgaac agatggatta catcaacgac
     5461 ataacggtac cacgcctgca aaaagtcatc ggaggtgaac agggggggat ctcaaaagac
     5521 atcaactggc agggtggcgg cagctttgtc tacgctgaac tgatggagct gaacgcctac
     5581 tttgtgcatg aaatacagaa agcgcagtcc actgaagaac tcgaaaagct ctttgccgtg
     5641 atgaaaacag aggcacatct caactatcag gtggcactgg agaacgtgct gtcggctgag
     5701 tacgaagtgg atggtatttt ccgtaaagtc gccttcagcg aactggaact gcatgagcag
     5761 aagcaactgc tgattgaaat cctcgataaa aatcagctct atgtgaacgc ttccgatatg
     5821 gatgacagtg acctgaacat ttcagagagc gataaagcct ttacccgcag cttctacgga
     5881 atggagtgac ggcatggcga agaaaccgac ctcagccggc agcgagaagc tgctgtttaa
     5941 caagctgaag caatttgatg aaccggggct gttccacgat aactaccaga cgccggacta
     6001 cattcaggcg aatctgaagg atgccctgcg tccgtatcag cacggtgcac tgcgttacct
     6061 gcattacacg cagcgtaaac gtgaagaggc gctgctgcat taccgtcatc tgctgtttca
     6121 catggcaacc ggtgcaggga aaacgatggt gatggcgggg acgatactct atctgtttaa
     6181 agagtacggg tatcagaatt tcatcttctt cgtgcatacc gacgcgatta tccagaaaac
     6241 ccgtgagaac ctgctgaatc cgcagtcgcc gaagtacctg ttcagtcagg agctggaaat
     6301 cgacggggaa aaaatcacca tcgcaccggt ggagacgttc ccgtccgtac cggaacgcaa
     6361 cacgatttac cttaagctct caaccatcca taagatgcac gatgagctga acagctatcg
     6421 tgagaacagc atcacctacg aggatttgaa ggaaattccg cttgtgctgc ttggtgacga
     6481 ggcgcatcac ttcaacgccg gcaccaaagc cagaggaaag tcgaaaacct caccggagaa
     6541 cgaggagcag acctgggaac gcacgattga gaacatcctc gacctgcgac cggataaccg
     6601 gctgttcgag ttcaccgcca ccattgacct ggcaaataaa gatatcgggc aaaaataccg
     6661 tgacaaagtg gtttatcagt atgacctgaa gcagttcatg tcagacggct attcgaagaa
     6721 agtcatgctg ctggaagcca accagaatga cggtgacaag atgctcgatg cggtactgtt
     6781 aagccagtac cgtaaactga cagcagcaga taacggcatc accggcttca agccggtcat
     6841 tctgttcaag tcgaacaaga ttgcgatatc aaaggcgaag caggaggagt tttcgcaact
     6901 gattgcggca atgacgcctg agagcgtcag acgccacctg gcgaacaaaa gagtacagct
     6961 cagctctgac accagtatct ggcacaaagt gattcagcgt tacgctgaca gcgatttagt
     7021 gacggttatc caacagattc aggaagactt taacgacttc aacctgctga acgtcaacaa
     7081 gtcagacctg ctggaagaaa accccgtcct gctgaacaca ctggaaaaca tcgataatcc
     7141 ggtgagagcc gtctttgccg tggcgaaggt caacgaaggc tgggacgtgc tcaacctcta
     7201 cgacattgtc cgcatcagcg agcaggcgag cagcagcaaa accggcacgg acagcgaggc
     7261 gcagcttatc gggcgtggtg cgcgttacta cccgttcgtt tatgacggta agcgcagctt
     7321 cacccgccgg tttgataaca gcgcgaaaga tttgagtgtg ctggagcaac tgcactatca
     7381 caccatcaat gaaccagctt atataaaaac gctgcacgca tcattagagc aggcggacat
     7441 tgacgtgcat caggatggca gcggcacgat tgaacacgcc agactgaaag aagacttcaa
     7501 gaaatccacg gtctatcaga cgggtaagct gtatttcaac aaagtggaag agattgagag
     7561 cagcagccgg cgctgggaaa cctactcgct ggaaacacgc tttgagatac cgtatcagac
     7621 cgccggtgag gagtcgctgg acaacctgac gggcgcaacg gcagtcataa caaaaccgga
     7681 acccctggta ctggacgagc gtttttaccg caaggcgatg cagaggatca gcttctacgc
     7741 gctggataac ctgcaacgct tcttcccgaa actgaccggc atccgtgagt ttattcgcag
     7801 tgacgcctat cttggcaagc taaaaatcac cgtcaccgta ccgcagtcac tggatttctc
     7861 ctctgtaccg gcaaaagaga aactgcacct gctggaaact gtgctgctgc gcatctcaga
     7921 gaacatacgc cgcaatgacc agaaggtgaa aggcacgtac cgctttatca gccagccggt
     7981 gaaggaggtg attaaagact acagcctgca cattgacccg tcggtggtca ttaaccagaa
     8041 aatcaccacc gcaccgacca tcggtaaaaa atggtacgtg tacgataacg cgatactgaa
     8101 ccagcttgag catcgccttg tgaaaacgct ggaagcattc atgccgaagc tgaaagcgcg
     8161 ttacgacgac atttacgtac tgcgtaacga cgagcagtca acgcgcttta aactcacgga
     8221 gttcggcggc gtgcgcgggt ttatgccgga tttcatcatg atactgacgc gccattcgga
     8281 caacacttac tggcaggtgt tccttgagcc aaagggggat gaccggctgc tggatgacgc
     8341 atggaaagag cagatgctgg aaacgctgaa tgacagggag cgcattgtga ttgatgaaaa
     8401 tgaggacgtc aggctggtcg ggattaaatt ctttgccaac agtcagatgg atgccttcgt
     8461 cagcgacatg cagaacaggc tgaatgaggg agaatcgctc gaaaccgcct cgttctccct
     8521 gccgttgtag gtcgcaacaa cacccaatag cggagcgtaa tgctccgtta ttttatttct
     8581 taataatcat ccaggtattc tctttatccc catacgtggt gcgggtgtcc accttaacgc
     8641 agggctttcc gtcacacgta gagatatcag ccagcggcgc ttttttctgc cagacagctt
     8701 ccatacgctg catttcagca atccggctgt acccgtcatt cataaaccat gtcagtgccg
     8761 tcacacagca agccaggacg aagaatatcg tcagcacaag ataaatcgcc gatttccacg
     8821 tatagcgata catttcacgg cgcatggcgt taacggagtc attagctgct ttcacgacct
     8881 gattaagggc attcacggcg ggcttctgct gctcagtgcc ggcttgtgtt aacgcctgcg
     8941 taacctgatt catagcggag ctgacagact ttctgactgt atcggtcata ttaccggacg
     9001 cactgtccag ctcccggata accttgctca gtgtttcgct ctgccgctgt aactgcacgg
     9061 aggcagcctg aaactcctca gtcagcgtgc tcactcgttt ttccagcgcc tgatgatgcc
     9121 cgataagggc agcaatctgt ttaatttcgt cgctcatatc aaatcctgct tatcgtgaga
     9181 atgaccagcc tttatcgggc ttctgtcgtt tctgttcggc aatgcgctgc cgctctttct
     9241 cctgctgacg ctgtttttcc gccagtctcg cccgctcttt ctgccgttcc atttcctgat
     9301 gtatctcctg aaactccgcc atcgcatcgt taacaaggga ctgaaggtca atttcttcct
     9361 gtatgtcctt catggcatcg ctgaccagtg cattcagctt ctccggctct ttttcaaaat
     9421 cgaataccct gccggaaacg gcgtcctgtt cgggatcaga tttctgcgtc tgttttagca
     9481 tcagagtgtc ccgctcactg atggcttccc gcagcgaggt ggttacgtca atcacgctgt
     9541 cacgttcatc acagcactgc tgaacctgtc tttccgcttc cctgcgctca agaatggcct
     9601 gtaactggtc agtatcgaag cgctgaacct gtcccgcccc cagatgccgc tccggttcac
     9661 gcccgatgcc ctgcgctttc agggagcggg catcgacacg gtcagcatgc tgatagcgtt
     9721 caagataggc attctggagg tcagcccagc gttcacgctg ggcgataaga tgctctttgc
     9781 gctctgtcgg tgtttccccg aaacgcacct ttttagctcc gccgcgctcc ggtactttag
     9841 agtttgcccg tttgaaatac tgctgcgggt cacggtcaat cccgtcattc atccgttcag
     9901 aaaacataat atgcgcgtgc ggctgttcgc cgccggcaat cgccgctgtc gggttatg
//
LOCUS       DAEVZA010000161         9815 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEVZA010000161 DAEVZA010000000
VERSION     DAEVZA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.161"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..428)
                     /gene="uidR"
                     /locus_tag="LAF92_004358"
     CDS             complement(<1..428)
                     /gene="uidR"
                     /locus_tag="LAF92_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBI9793275.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQ"
     gene            complement(657..1424)
                     /gene="hdhA"
                     /locus_tag="LAF92_004359"
     CDS             complement(657..1424)
                     /gene="hdhA"
                     /locus_tag="LAF92_004359"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBI9793276.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(1536..2564)
                     /gene="malI"
                     /locus_tag="LAF92_004360"
     CDS             complement(1536..2564)
                     /gene="malI"
                     /locus_tag="LAF92_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBI9793277.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            2739..4331
                     /gene="malX"
                     /locus_tag="LAF92_004361"
     CDS             2739..4331
                     /gene="malX"
                     /locus_tag="LAF92_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBI9793278.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVRA"
     gene            4341..5513
                     /gene="malY"
                     /locus_tag="LAF92_004362"
     CDS             4341..5513
                     /gene="malY"
                     /locus_tag="LAF92_004362"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBI9793279.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            5617..6618
                     /gene="add"
                     /locus_tag="LAF92_004363"
     CDS             5617..6618
                     /gene="add"
                     /locus_tag="LAF92_004363"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBI9793280.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(6653..7693)
                     /locus_tag="LAF92_004364"
     CDS             complement(6653..7693)
                     /locus_tag="LAF92_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI9793281.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7936..8061
                     /gene="blr"
                     /locus_tag="LAF92_004365"
     CDS             7936..8061
                     /gene="blr"
                     /locus_tag="LAF92_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBI9793282.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            8334..8549
                     /gene="ydgT"
                     /locus_tag="LAF92_004366"
     CDS             8334..8549
                     /gene="ydgT"
                     /locus_tag="LAF92_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBI9793283.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            8635..9075
                     /locus_tag="LAF92_004367"
     CDS             8635..9075
                     /locus_tag="LAF92_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBI9793284.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            9152..9733
                     /gene="rsxA"
                     /locus_tag="LAF92_004368"
     CDS             9152..9733
                     /gene="rsxA"
                     /locus_tag="LAF92_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBI9793285.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            9733..>9815
                     /locus_tag="LAF92_004369"
     CDS             9733..>9815
                     /locus_tag="LAF92_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBI9793286.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRR"
BASE COUNT         2260 a         2506 c         2422 g         2627 t
ORIGIN      
        1 ttttgctggg catcacgcat ccgctgggca acaaattcag tgatcgtcat atgcttattt
       61 ttaagcatgg cggcgacctg tgggttacgc atcccttccg ccataatttc gaccaccagc
      121 gcccgttgcc caaaggcttc atgggtgaga gagacaatag actcgaccat atagtcaaca
      181 aaatgaatcc cttcaatcgg ttcccggaaa cgggccagcg ccctctcctg gtcctgtaag
      241 ataatcgcct gaatcaaggc ttctttggag atgaaatggt gatagagcgt cccgggacta
      301 atggcgcagg atttacagat ggctttcatc gaggcactgt gaaatccatt ttctgaaaaa
      361 atctctctgg cagcattgag gatccgggtc cgtgtcggtt gtgcttcagt ctgcatgtta
      421 tccatcatta aaccattata gttagcgtga ataactagtt taccgcgttg tcaggtgagc
      481 gcctatcatt gctgttgaat agtgcgaagg cacactctat tcgtatgctt ttcatccaga
      541 tattggttaa ccaatagccg tgatttggct gccagttgtt tagcagagac gttgatgaat
      601 tgcacacgct atcccggtaa acaacacata cggctgttta ccggttcgtt agtgtattaa
      661 ttgagctcct gtaccccacc accggagacg gtgagaattt gtccgcttac ccagctggct
      721 gcaggcgagc aaaggaacag cgccgcgtta gcaatatctt gcggttggcc cagacgtctg
      781 atcggcgtgt gctgtaacat tttttgttca atttctggtg taataacgga tttcagggca
      841 tcggttaata ttgcccccgg cgcaatgcca ttcacccgaa tatttttttc acccaggtca
      901 aatgccatat ttctgaccag atgactggcc gcagctttag atgatgcata ggaagtcatg
      961 tttatatttt tattttctgc cgccatagaa gtgatggtca gaataacgcc accgccattt
     1021 ttttccattt ctggcgcaac aagttgtgac agatggaaaa aagaaaacac attcagttca
     1081 taagcacggc gaaaatctgc cattggcata tcaaacggtt taggaccacc gccaccggcg
     1141 ttgttaacca ggatatcaac tttacccagc ttactgacgg caaagtctgc cagtgcagag
     1201 agttcctgtt cggaagtaat atcacaacgg caggcaaatg cctgaccacc cagttgttga
     1261 atttcgtcta caacatggtt agctgcgtcg gcgttaatat cactgaccac cacagatgcg
     1321 ccagctgtcg cgaatgtaat ggcgatttct ttaccaatac ctgcacccgc acctgtgatg
     1381 atggcgcatt ttccgtcgag tctcaggttg tcagaattaa acacataaac ctcctgtgaa
     1441 cttcagtaaa aataaaaggc accgcctgtg tgccacattt tttatcatta accttgagga
     1501 aaggacacta ccaggtatag cagataaaga gaggattatt tcgccgcaat gagccgggcg
     1561 ggaataataa gattgcgtga atgcgtctct tcatgggtga ttttttgcat catgcgatcc
     1621 gcaagtgtgg taccaagttc ccgcgcaggc gtgctggccc aggtaacggg tatatcatca
     1681 agtgtggttg gtgtcgcatc ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga
     1741 tcgactccgc tttccccgct ttgccgccct gctttgagca aaccaaacca tgcccccatc
     1801 gcaatagttt cgttatagca aaccacagca ctgatggtcg ggttatgacg taaaagcgcc
     1861 gtgatagctt ccgcggcttg cttctggctg gatgtgcact ccagcaccca atcgctgtga
     1921 aacggcaggc caaattttag tagagttgca caatagcccc ccacccgttc tgcacgggtt
     1981 aatgaggaac tttgccctcc cagccaggcg atccgctgat gcccattgcg aatgagatgc
     2041 tccgtcaaca actgtgcagc ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga
     2101 taactggcac gggaagcgaa aatcaccggg atagcttttt cttctgccat ccgtcgcagg
     2161 tcatcgctgc ttcctgcagc cccggcaatt accacaccat ccacaccctg attcagtaac
     2221 agtgaaaacc gctgtgccag ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc
     2281 cgtccctgcg cttccagagc ttccgtcaat ccggccgtca attcggcgta aaacggcgca
     2341 gataaatcac ggacgatcaa accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc
     2401 tggcgattgc gcacaaatcc cagctcttca atggcggcgt taacgcgttc tcctgtggcg
     2461 gtagagattc gccctttgcc actaagcacc agcgaaacgg tacttaccga cacgcccgca
     2521 gccagcgcaa catcatgaat ggttattttt ttggcggtag ccatgcagat gacctactcc
     2581 ctgattatgt gacagataaa acgttttacc ttttatttat cttatacccg ctattatcgt
     2641 tgcgtaatgt gatttatgcc tcactaaaat ttgataaaac gttttatctt ctcgcgcaat
     2701 ttactgaatc cagattgttc tctacgagga gtcgttttat gacggcgaaa acagcaccga
     2761 aagtcacgct gtgggagttc ttccagcagt taggcaaaac cttcatgtta cccgtggcat
     2821 tattgtcgtt ctgcggcatt atgctcggca ttggtagttc tcttagcagc catgatgtca
     2881 taaccctgat cccggtcctg ggcaaccccg tgttgcaggc tatctttacc tggatgagta
     2941 agattggctc gtttgctttt agtttcctgc ctgtcatgtt ctgtatcgcc atcccgctgg
     3001 gcctggcacg cgaaaataaa ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa
     3061 tgaacctcgc ggtaaacttc tggttgacca ataaaggcat tctgccaacc acggatgccg
     3121 cggttctgaa agccaataac atccagagca ttcttgggat ccagtcgatc gataccggga
     3181 tcctcggtgc ggtgatcgcc ggtattatcg tctggatgct gcatgagcgt ttccataata
     3241 tccgcctgcc ggatgcgctg gcattcttcg gcggtacgcg cttcgtacca attatctcct
     3301 cgctggtgat gggccttgtc ggcctggtga ttccattagt ctggccgatt ttcgccatgg
     3361 gtattagcgg cttgggccat atgataaaca gcgcgggtga tttcggaccg atgctgtttg
     3421 gtaccggtga acgtctgctg ttgccgtttg gtctgcatca cattctggtg gcattaattc
     3481 gctttaccga cgcaggcggc acgcaggaag tctgcggtca aaccgtcagc ggcgcactga
     3541 ccatcttcca ggcgcaattg agttgcccga ccactcacgg tttttctgaa agcgccacgc
     3601 gtttcctttc gcaaggtaaa atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag
     3661 ctatgtatca ctgcgcgcgc ccggaaaatc gccataaaat taaaggtctg ctgatttctg
     3721 gcctgatcgc ctgtgtcgtt ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg
     3781 tggcgccagt tctgtatgtc atccacgcgc tgttaaccgg cctcggcttc actgtcatgt
     3841 ctgtgctcgg cgtcaccatc ggtaataccg acggcaatat catcgacttc gtggtgttcg
     3901 gtattttgca tggtctgtca accaagtggt acatggtgcc agtggtggcg gcaatctggt
     3961 ttgtcgttta ctacgtcatc ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg
     4021 ggcgcgatag cgaagttgcc agctcaatcg aaaaagccgt tgccggtgcg ccgggtaaat
     4081 caggttacaa cgttcctgca atcctcgaag cattaggcgg tgccgacaat attgtcagcc
     4141 tcgataactg cattacccgt ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc
     4201 aggcactgaa ggacaatcgg gcaattggcg tagtacaact taatcaacat aacctgcagg
     4261 ttgttatcgg gccacaagtt cagtcagtaa aagatgaaat ggccggtctg atgcatactg
     4321 tccgggcata aggataagat atgttcgatt tttcaaaggt cgtggatcgt catggcacat
     4381 ggtgtacaca gtgggattat gtcgctgacc gtttcggcac tgctgacctg ttaccgttca
     4441 cgatttcaga catggatttt gccactgccc cctgcattat cgaggcgctg aatcagcgcc
     4501 tgatgcacgg cgtatttggc tacagccgct ggaaaaacga tgagtttctc gcggctattg
     4561 cccactggtt ttccacccag cattacaccg caattgatac gcagacggtg gtgtatggcc
     4621 cttctgtcat ctatatggtt tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg
     4681 tggtgatcca cacgcccgcc tatgacgcat tttacaaggc cattgaaggt aaccagcgca
     4741 cagtaatgcc cgttgcttta gagaagcagg ctgacgactg gttttgcgat atgggcaagc
     4801 tggaagccgt gttggcgaaa ccagaatgta aaattatgct cctgtgtagc ccacagaatc
     4861 ctaccgggaa agtgtggacg tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc
     4921 atggtgtgcg ggttatttcc gatgaaatcc atatggatat ggtctggggc gagcagccgc
     4981 atattccctg gagtaatgtg gcgcggggag actgggcgtt gctgacgtcg ggctcgaaaa
     5041 gtttcaatat tcccgcgctg accggtgctt acgggattat agaaaatagc agcagccgtg
     5101 atgcctattt atcggcactg aaaggacgtg atgggctttc ttccccttcg gtactggcgt
     5161 taactgccca tatcgccgcc tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct
     5221 atctgaaaga taacctgacg tatatctcag ataaaatgaa cgccgcgttt cctgaactca
     5281 actggcagat cccgcaatcc acttatctgg cctggcttga tctacgtccg ttgaatattg
     5341 acgacaacgc gttgcaaaaa gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt
     5401 atacctatgg tgaagaaggt cgtggttttg tccgtctcaa tgccggctgc ccacgttcga
     5461 aactggaaaa aggtgtggct ggattaataa acgccatccg cgctgttcgc taaccccaat
     5521 tgcgcaacgt aaaaaatcgt tgcgcaatcg tggattttta ccctgctttg tttttataat
     5581 ggtgcgcact tttatattca gaaaaagagt gcgaccatga ttgataccac cctgccatta
     5641 actgatatcc atcgccacct tgatggcaac attcgtcccc agaccattct tgaacttggc
     5701 cgccagtata atatctcgct tcctgcacaa tccctggaaa cactgattcc ccacgttcag
     5761 gtcattgcca acgaacccga tctggtgagc tttctgacca aacttgactg gggcgttaaa
     5821 gttctcgcct ctcttgatgc ctgtcgccgc gtggcatttg aaaacattga agatgcagcc
     5881 cgtcacggcc tgcactatgt cgagctgcgt ttttcaccag gctacatggc aatggcacat
     5941 cagctgcctg tagcgggtgt tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc
     6001 acctttggtg tgcaggcgaa gcttatcggc attatgagcc ggaccttcgg cgaagccgcc
     6061 tgtcagcaag agctggaggc ctttttagcc caccgtgacc agattaccgc acttgattta
     6121 gccggtgatg aacttggttt tccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt
     6181 gatgcgggct ggcatattac cgtccatgca ggcgaagctg ccgggccgga aagcatctgg
     6241 caggcgattc gtgaactggg tgcggagcgt attggacatg gcgtaaaagc cattgaagat
     6301 cgggcgctga tggattttct cgccgagcaa caaattggta ttgaatcctg tctgacctcc
     6361 aatattcaga ccagcaccgt agcagagctg gctgcacatc cgctgaaaac gttccttgag
     6421 catggcattc gtgccagcat taacactgac gatcccggcg tacagggagt ggatatcatt
     6481 cacgaatata ccgttgccgc gccagctgct gggttatccc gcgagcaaat ccgccaggca
     6541 cagattaatg gtctggaaat ggctttcctc agcgctgagg aaaaacgcgc actgcgagaa
     6601 aaagtcgccg cgaagtaaca aaatggatgg tgcaaattgc accatccatt tttcatgcaa
     6661 ggcacaaagt cgcgcgatgt ttggcggatt cgatgcccag ctcaatcaac tccattacct
     6721 ggattgcctg gcttgccgga accggatttt caccatcgcc atttaacgca tcacgaatag
     6781 ccgcatagta agccggataa ttcccaggca ccgtcaacag cgtttcttcg acacgttcct
     6841 caccttccac gcgggtaagt acgccatcac gcatatcgta gccccagtct tcctgcggta
     6901 gacgctcgcc atttttcaga cgttcttcct gtggatcgag gccatatttc acataactgc
     6961 ctcgggatcc atgcacgata taccgtgctg actcagcggc tgccagcatg gtaccgtgta
     7021 aaatgactcg ccgctgtgga taggacaaga tggcgtggaa ataatcggtc gactgcgctc
     7081 cgggccgtaa ctgtgccaaa tctaccgtca tgctgaccgg taaaccaaaa agcgtaatgg
     7141 cctgatcaag aagatgtggt gctaaatcgt accagatacc gctgcctggg ccgccctgtt
     7201 cacgccaacg atcgcgcacc tgcggacgga agcggtcaaa atgagactca aagtaagcaa
     7261 cttcacccag cacgccttcc gcgagtaaac cttttagcgt caagaaatcg ctatcccagc
     7321 gacggttatg gaatacagac agcacacgcc ccaggctttt tgccagtgca tccagctctc
     7381 gcgcttgtga cagtgtcacg gtaaagggtt tatcaacgac cacatgttta cccgcctcaa
     7441 gcgccgcttt ggctaacggg aaatgggtat cgttgggtgt aggaatgaca atcaggtcta
     7501 tgttgggatc gttaaacaga tgcttcggct cagagacaac cgtaaccgtt ggccagtcgg
     7561 cttttacttt tgtttcatca ctgctggaga ttaccgccag ttccagcccg ggcgtgcccg
     7621 caatcagggg cgcatggaag gttttgctcg cataaccata cccaatcaac ccaacacgga
     7681 tgttgtcgct catgtcattg cctctcattg aagtatgatg gctatttgac actatccttt
     7741 acccacgctc aacagtttaa taacctgcca gcaataaggg atgttgttta acttaagtca
     7801 aaaaaatagc gaattttcca acgacaaaag ctaaatatcg caaaaacctc agtaaaaatc
     7861 ttgctggagc tattattgct aagtaacatt taccccctga agttaatgga tcaatcaaga
     7921 gagatgtggg ctgtaatgaa tcgtcttatt gaattaacag gttggatcgt tcttgtcgtt
     7981 tcagtcattc ttcttggcgt ggcgagtcac attgacaact atcagccacc tgaacagagt
     8041 gcttcggtac aacacaagta agctctgcac ttgtggagcg acatgctgcc cgtccgggtg
     8101 catgttttca cttgtcggat attaaaccag gaatttatta tcttgttcga tgttgttggt
     8161 gattgtcagg gatagtaaag cgcgttactc ttttcagagc agtttttcat ccctgaatat
     8221 ttgccatcag cgcattctta ttattgtaat aacacaagcg ttcacttttg gtgaaatttt
     8281 atcatcacta ataataccta cccctttggt attaattatt ttatggacct tttatgactg
     8341 ttcaggacta cttattaaaa tttcgcaaaa tcagttcact cgaaagtctg gaaaaactct
     8401 acgaccatct taattacacc ctgacggacg atcaggaact gatcaatatg tatcgtgctg
     8461 ccgatcaccg tcgcgcagag ctggtttctg gcgggcgttt gtttgacctc ggccaggtac
     8521 cgaagtccgt ctggcactat gtccaataaa gaaagtagcg atcggttata aaagccttat
     8581 aataacgccc ctaaaattag atttgcctgt ttgcgtcgct attctggaga acctatgacc
     8641 accacaacgc cacaacgtat tggaggctgg ttgcttggcc cgttggcctg gcttttagtc
     8701 gctctgttga gtacgacgct ggcgttgttg ctgtataccg ctgcgttatc ttctccgcaa
     8761 acatttcaaa cgctcggcgg acaagcactg acgacacaaa tcttatgggg cgtttctttt
     8821 attaccgcta tcgccatgtg gtattacaca ctgtggttga ccatcgcttt ctttaaacgt
     8881 cggcgttgtg ttcctaaaca ctatattatc tggctgctta tttccgtatt gctggcagta
     8941 aaagcattcg ctttttcacc cgtggaagat ggcatagccg ttcgccagtt gctgtttact
     9001 ttgttggcaa ccgcattgat cgtcccctat ttcaaacgct cgtcgcgggt aaaagcgacg
     9061 tttgtgaatc cgtaataacc ttacagttaa cctgttgtcg cctgctctgg attaacggat
     9121 aataggcggc ttttttattt caggccgaaa aatgactgac tacctgttac tgtttgtcgg
     9181 aactgtactg gtcaataact ttgtactggt caagtttctc ggtctctgtc cgtttatggg
     9241 ggtttccaaa aaactggaaa ccgcgatggg catggggctg gcaacaacgt ttgtgatgac
     9301 gctggcgtct atttgcgcct ggcttatcga tacgtggatt ttgatcccac ttaatctgat
     9361 ttacctgcgc accctggcat ttattctggt gattgctgtg gtcgtgcagt tcaccgagat
     9421 ggtggtgcgc aaaaccagcc cggtgcttta ccgtctgctg gggatttttt tgccgcttat
     9481 caccaccaac tgtgcagtgc tcggcgtggc gttgctgaat atcaatctcg ggcacaattt
     9541 cttgcagtcg gcgctgtacg gtttttccgc cgctgtcggt ttctcgctgg tgatggtgct
     9601 cttcgccgcc atccgcgaac gccttgctgt ggctgatgtc ccggcacctt ttcgcggtaa
     9661 tgccattgcg ttaattaccg caggtcttat gtctctggcc tttatgggct ttagtggttt
     9721 ggtgaagttg taatgaatgc tatctggatt gccgttgccg ccgtgagcct gctgggcctg
     9781 gcgtttggcg ccattctggg ttatgcctcc cgccg
//
LOCUS       DAEVZA010000162         9809 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEVZA010000162 DAEVZA010000000
VERSION     DAEVZA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.162"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..435)
                     /locus_tag="LAF92_004370"
     CDS             complement(<1..435)
                     /locus_tag="LAF92_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBI9793287.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEY"
     gene            543..848
                     /locus_tag="LAF92_004371"
     CDS             543..848
                     /locus_tag="LAF92_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBI9793288.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            1047..3473
                     /locus_tag="LAF92_004372"
     CDS             1047..3473
                     /locus_tag="LAF92_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBI9793289.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVIIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            3534..5957
                     /locus_tag="LAF92_004373"
     CDS             3534..5957
                     /locus_tag="LAF92_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBI9793290.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            5968..6585
                     /gene="dmsB"
                     /locus_tag="LAF92_004374"
     CDS             5968..6585
                     /gene="dmsB"
                     /locus_tag="LAF92_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBI9793291.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            6587..7441
                     /locus_tag="LAF92_004375"
     CDS             6587..7441
                     /locus_tag="LAF92_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBI9793292.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            7484..8098
                     /gene="dmsD"
                     /locus_tag="LAF92_004376"
     CDS             7484..8098
                     /gene="dmsD"
                     /locus_tag="LAF92_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBI9793293.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            8257..9549
                     /gene="clcB"
                     /locus_tag="LAF92_004377"
     CDS             8257..9549
                     /gene="clcB"
                     /locus_tag="LAF92_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBI9793294.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(9502..>9809)
                     /locus_tag="LAF92_004378"
     CDS             complement(9502..>9809)
                     /locus_tag="LAF92_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9793295.1"
                     /translation="VVQEQLPVLMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRI
                     NPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV"
BASE COUNT         2394 a         2514 c         2505 g         2396 t
ORIGIN      
        1 atattccatc tgcatttgtt tgctgctggc tttaatcacg aagccattat catcggtttc
       61 ccaactcacc ccggcagaag gtaattctgc tagctggcat tttccctgta aacgtactct
      121 cttctccagc gtctcggcat cacggtaata attggcgtcc agtaccagag cgaccacggt
      181 attattttcc agatccagta attcgagtga atcaaaacag ccttcttccg acaaagtccc
      241 agaaacacgt ttcgtcactt caccttgctc atccattaat gtctgagtga aatcttttac
      301 cggaccacgc agcggatcaa aatcgaattc attagagaaa ctggccatct caggggtaaa
      361 tgaaagtgtt acctctgtgc ggtcacatcc tgtgaggaat atcgcgagta agcatggtag
      421 taatttgtat ttcacaacag tcaccagaga gtagagatga ttctcaatca tagtagcaaa
      481 tacagtactt tacacgttaa atgctatgct taaagaagtt atcttcgcgt aaggagctta
      541 tgatgaaact ctcaacctgc tgtgccgcgc ttctgctcgc cctcgcctca cccgcggtac
      601 tcgccgcgcc gggatcctgt gagcgcatac agagcgatat atcacagcgc attatcaata
      661 atggcgtacc cgaaagcagc ttcacgttaa gtattgtacc caatgaccag gttgatcagc
      721 ctgattccca ggtcgtcggg cattgtgcta atgatacgca taaaattctc tacacccgca
      781 ccaccagcgg taacgtctct gctcccgcgc agtctaccca ggatggcgcg cctgccgaac
      841 cgcagtaata cattatcgac tgaacgccgg atacgacaca tccggcgatt tgaacataca
      901 acataatccc accttattac tcataccctt ctattgatat ggattaataa ttcttaaccc
      961 aaaatgggta gactccctct attgttagcg cgctaaatat tcaatatata aacatttata
     1021 taacgataaa gaacagggag tgagttatgt ccaaaaatga ccgaatggtg ggcatcagcc
     1081 gcagaacact cgttaaatca accgcgatag gttctctggc gctggctgca ggcggttttt
     1141 ctttaccgtt caccctgcgc agtgcagcgg cagcggtaca acaggccagc gaaaaagtga
     1201 tctggggtgc ctgttccgtc aactgtggta gccgctgtgc acttcgtcta catgttaaag
     1261 ataatgaagt gacctgggtg gaaactgaca ataccggcag cgatgagtac ggcaaccatc
     1321 aggtacgcgc ctgtttgcgc ggtcgctcca tccgccggcg tattaatcat cccgatcgct
     1381 tgaattaccc aatgaaacgc gtgggtacac gcggtgaagg caaattcgaa cggattagct
     1441 gggatgaagc cctggatact atcgccagta gcctgaaaaa aaccgtcgaa caatatggca
     1501 atgaggctgt atatattcag tactcttcgg ggattgttgg cggcaatatg acccgctctt
     1561 cgccatcagc ctcggcggtc aaacgcctga tgaactgcta cggcggttca ctcaatcagt
     1621 atggctccta cagcaccgcg caaatttcct gtgccatgcc ctacacctac ggcagtaatg
     1681 atggcaacag caccacggat attgaaaaca gcaagctggt cgtgatgttt ggtaacaacc
     1741 cggcagaaac ccgcatgagc ggcggtggca tcacttatct tcttgaaaaa gcgcgcgaga
     1801 aatcgaacgc caaaatgatt gttatcgatc cgcgatatac cgatacggct gctggtcgtg
     1861 aagatgaatg gctccctatt cgcccgggca ccgatgccgc gctggttgcg ggtattgcct
     1921 gggtattgat taacgaaaat ctcgttgatc aaccttttct cgataaatac tgcgtcggct
     1981 atgacgaaaa aaccttaccc gcagatgcac ccaaaaatgg tcactataaa gcctatattc
     2041 ttggtgaagg tgatgataac acagcgaaaa cgccgcagtg ggcttcgcaa attaccggta
     2101 tcccggtaga ccgtatcatc aaactggcgc gtgaaattgg cacagcaaaa cccgcttata
     2161 tctgccaggg ctgggggccg caacgccagg caaacggcga actgactgca cgcgctattg
     2221 ctatgttacc tattttgacg ggcaatgtcg gcatcagcgg cggaaatagt ggcgcgcgtg
     2281 aatcgaccta taccattacc atagaacgcc tgccggtgct ggataatccg gtcaaaacgt
     2341 caatctcctg cttcagctgg acagatgcta tcgatcatgg tccgcaaatg acggcaatcc
     2401 gcgacggcgt ccgcggcaaa gataaactgg atgtgcccat taagttcatc tggaactacg
     2461 cgggaaatac cctcgttaat cagcattctg acatcaacaa aacgcatgaa attctgcagg
     2521 acgaatcgaa atgcgaaatg attgtgatca tcgaaaactt tatgacctca tcggcaaaat
     2581 atgccgacat tctgctgcca gacctgatga ccgttgagca ggaagatatt attcctaacg
     2641 actacgccgg taacatggga tatctcattt tcctccagcc tgtcaccagc gaaaaattcg
     2701 aacgcaaacc gatttactgg atcctgagtg aagtcgcgaa acgtcttgga cctgacgtct
     2761 atcaaaagtt cacagaaggt cgcacgcagg aacaatggtt acaacatctg tacgccaaaa
     2821 tgcttgccaa agatccggcg ttaccgtctt acgacgaact gaaaaaaatg ggtatctata
     2881 agcgtaaaga tcccaatggc cattttgtcg cctacaaagc atttcgtgac gaccccgagg
     2941 caaatccact taaaacgcct tccggtaaga ttgaaattta ttccagcagg ctggcggaaa
     3001 ttgcccgtac ctgggaactg gaaaaagatg aagtgataag cccattgcca gtttatgcct
     3061 caacctttga aggctggaac tcccctgaac gtagaacctt cccactgcaa ctgtttggtt
     3121 tccattacaa atcccgtact cactcgacct acggcaatat tgatctcctg aaggctgcct
     3181 gccgtcagga ggtgtggatc aaccctatag atgcgcagaa acgtgggatt gccaacggcg
     3241 atatggtgcg ggtgtttaac catcgtggcg aagttcggct accagccaaa gtaacaccac
     3301 gtattctccc tggagttagc gctatgggcc agggagcctg gcacgaggcc aatatgtctg
     3361 gcgataaaat cgaccatggc ggctgtgtga atacgctaac cactctgcgc ccttcaccac
     3421 tggcgaaggg aaacccgcag cacactaatc tggtcgagat cgaaaaaata taacccacga
     3481 caaccataaa ttctggcatg acattttgtt tgaaaagcaa taagtgagta atgatgaaaa
     3541 tccataccac agaggcgctc atgaaggctg aaatcagccg tagaagtctg atgaaaacct
     3601 ccgcacttgg cagtcttgcg ctggcaagca gtgctttcac tctgccattt tcccaaatgg
     3661 tccgggcggc agaggctccg gtagaagaga aagcggtctg gagttcctgc accgttaact
     3721 gcgggagccg ctgtctgtta cgtttgcatg tgaaagatga caccgtgtac tgggtggagt
     3781 ctgatacgac aggtgacgac gtctacggta accatcaggt tcgagcgtgt ttacgcgggc
     3841 gctctatccg ccgacggatg aatcatcctg ataggttgaa atatcccatg aagcgcgtcg
     3901 gcaagcgcgg tgaaggtaaa tttgaacgga taagttggga cgaagccctg gataccatca
     3961 gtgataatct tcggcggatc ctgaaagatt acggcaatga ggctgtacat gtcctgtacg
     4021 gaacaggcgt agatggcgga aacatcacca actcaaacgt cccgtaccgt ctgatgaact
     4081 cttgcggtgg ttttctcagt cgctatggca gctacagtac cgcacagatc agtgccgcaa
     4141 tgagttatat gttcggtgcc aatgatggca acagcccgga tgatatcgcc aatacgaaac
     4201 tggtcgttat gttcggaaat aacccggcag aaacgcggat gagcggcggt ggtgtcactt
     4261 actacgtcga gcaagcccgc gaacgttcaa acgcacgcat gatcgtcatc gatccacgtt
     4321 ataacgacac tgctgccggg cgtgaagatg aatggctgcc cattcgccct ggcaccgatg
     4381 gcgcactggc ctgtgcgatt gcctgggtac tgattactga aaacatggtc gatcagccat
     4441 ttctcgacaa atattgtgtt ggttacgatg aaaaaacgct gcccgccaac gcaccacgta
     4501 acgcgcatta taaagcctat attctgggcg aagggcctga cggcatagct aaaacgccgg
     4561 aatgggcagc aaaaatcacc agcatcccgg cagaaaaaat tatccagttg gcacgagaga
     4621 tcggttcagc aaaacctgct tatatttgtc agggttgggg gccacaacga cattccaacg
     4681 gcgagcaaac gtcccgcgct attgccatgc tttccgttct caccggtaac gtcggcataa
     4741 acggcggcaa ctcaggtgta cgcgaaggta gctgggatct gggggtagaa tggttcccga
     4801 tgcttgagaa tcctgttaaa acgcagattt ccgtctttac atggacagat gccatcgacc
     4861 atggtacgga aatgaccgcg acccgtgatg gtgttcgtgg aaaagaaaaa ctggatgtcc
     4921 ccatcaagtt tttatggtgc tacgccagta acacattgat caatcaacat ggcgacatca
     4981 atcacaccca tgaggtgctt caggatgaca gcaagtgcga gatgattgtt ggcattgacc
     5041 acttcatgac ggcctcggct aagtattgcg atatcctgtt gcccgacctg atgccaacag
     5101 agcaagaaga ccttatctct catgaatctg cagggaatat gggctatgtg atcctcgccc
     5161 aacccgcaac ctcagcaaaa tttgaacgca aacccatcta ctggatgctg agtgaagtcg
     5221 ccaaacgctt aggaccagac gtttatcaaa cctttactga aggtcgcagt cagcatgaat
     5281 ggatcaaata tctccatgcg aaaacgaagg aacgtaaccc tgagatgccc gactacgagg
     5341 agatgaaaac gaccgggatc tttaagaaaa aatgcccgga agaacactac gtcgctttcc
     5401 gcgcattccg tgaagatcca caggcaaacc cgttgaaaac accttcgggg aaaatcgaaa
     5461 tttattctga acgactggcg aagattgcag atacctggga attgaaaaaa gatgaaatta
     5521 ttcatcccct tcctgcgtat accccaggtt ttgatggctg ggacgatccc ctgcggaaaa
     5581 cctatccact gcagttaacg ggcttccact acaaagcgcg tacccactcc agctacggca
     5641 atattgatgt gttacagcag gcctgcccac aagaggtgtg gatcaacccc attgatgctc
     5701 aggcacgcgg tatccgtcat ggcgataccg tgcgggtatt taacaataat ggagaaatgc
     5761 tgattgccgc aaaagtgact ccgcgtattc tgcctggcgt taccgccatc gggcaaggtg
     5821 cgtggcttaa agcggatatg tttggtgacc gggtcgatca cggcggcagt atcaatattc
     5881 tgacctctca ccgcccttca ccgctggcaa agggaaaccc gtcgcacagc aatcttgtcc
     5941 agatcgaaaa ggtttaagga gtagcccatg accacacaat atggattttt tatcgattcc
     6001 agccgctgta ctggctgtaa aacctgcgaa ctggcgtgca aagattttaa agatcttggc
     6061 ccggaagtca gtttccgccg tatttatgaa tacgctggcg gcgactggca ggaagataat
     6121 ggcgtctggc accagaacgt gttcgcttat tatctctcca tctcctgtaa ccattgcgat
     6181 gaccccgcat gtacaaaagt ctgccccagc ggcgcaatgc ataagcgtga agatggcttt
     6241 gtggtggttg atgaagatgt ttgtatcggc tgccgctact gccacatggc ttgtccgtac
     6301 ggcgcaccac agtacaatgc tgaaaaaggg cacatgacga agtgcgatgg ttgttattcg
     6361 cgcgtcgccg aggggaaaca acccatatgt gtcgaatcct gcccgctgcg tgcacttgag
     6421 ttcggtccaa ttgaagagct gcgccagaag cacggcactc tggctgccgt cgcgccactg
     6481 ccgcgcgcgc atttcacaaa acccaatatc gttatcaaac ctaacgccaa cagccgcccg
     6541 accggcgata ccactggtta tctggctaat ccggaggaag tgtaaaatgg gaaatggatg
     6601 gcatgagtgg ccactggtta tctttaccgt attgggtcaa tgcgtagtag gcgcgctgat
     6661 tgttagcgga attggctggt tcgccgccaa aaatgacgca gaccgtcagc gtattgtccg
     6721 tggcatgttt ttcctctggc tgttgatggg catcgggttt attgcctctg tcatgcacct
     6781 gggatcgcca ctgcgagcgt ttaactcgct gaatcgaatc ggagcttctg gtctgagtaa
     6841 cgagattgct gccggttcca tattttttgc cgttggcgga ctatggtggc tggtggcggt
     6901 catcggtaaa atgccgcaag cgttggggaa actctggtta cttgtcagca tggcgctcgg
     6961 cgtcattttc gtctggatga tgacctgcgt gtatcaaatc gacaccgtgc caacctggca
     7021 taacggttac actacgctgg ctttcttcct gacggtattg ttgagcggtc caatacttgc
     7081 tgccgcaatt ctgcgggcag ctcgcgttac atttaatacc acaccatttg ccatcattag
     7141 cgttttggcg ctgatcgctt gtgccggggt gattgtgctg caaggtttgt ctctggcgtc
     7201 tattcactca tccgtgcaac aagccagcgc actggtacca gattacgcct ccttacaggt
     7261 ctggcgtgtg gtattgttat gcgcaggtct tggatgctgg ctttgtccgc tcattcgtcg
     7321 ccgtgaacct cacgttgccg gacttattct ggggctaatc ctcatacttg gcggtgagat
     7381 gatcgggcgt gtgctctttt atggcctgca catgacagta ggaatggcta ttgcaggtta
     7441 acaaattggt gcggggcaac ccgcacattt caggatgtta ggaatgaccc atttttcaca
     7501 gcaagataat ttttctgtcg cggcgcgcgt gttgggcgcg ctgttttatt acgctccaga
     7561 gagtgcggaa gccgcacctc tggttgcggt actcaccagt gacggctggg aaactcagtg
     7621 gcctctacca gaagcgtcat tagcgccgct ggtgacggct tttcagacac agtgtgaaga
     7681 gactcacgcc caggcctggc agcgtttgtt tgtcggcccg tgggcactgc cgtctccgcc
     7741 gtgggggtcg gtctggctgg atcgcgaatc tgtgctgttt ggcgattcaa cattggcact
     7801 tcgtcagtgg atgcgcgaga aaggcattca gtttgaaatg aagcaaaacg aaccggaaga
     7861 tcattttggt tcactgctgt tgatggctgc gtggttggcg gagaatggtc gccagacaga
     7921 atgcgaagaa ttgctggcat ggcacctttt tccgtggtca acacgttttc ttgatgtttt
     7981 tatcgaaaaa gcagaacacc ctttctaccg tgcactgggt gaactggctc gcttaacgct
     8041 ggcgcaatgg caatcacaac tgttgattcc tgtcgcggtt aaaccgctgt ttcgatagga
     8101 tcaccgtaat attgccggat ggtgacgatt acgcgcaatc cggcaataat aggttacagt
     8161 gtcacgtttt tttatctctt aaagcacgca ctgcttttgc ggctggcctc ttttgtcgca
     8221 aaatagtcgc ccgtgtttca ttgcccattt ctgctcatgc atcatctaca catctatccg
     8281 gatctgcgca ctatgttccg ccgtctgctt atcgcaacag tcgtcggtat tctcgcggcc
     8341 tttgccgttg ccgggtttcg tcatgcgatg ctgctactgg agtggttgtt cctcaataat
     8401 gactccggca gtctggtcaa tgcagcgaca aacctttccc cctggcgacg gttgctaact
     8461 ccggcgctcg gcggactggc ggcgggtttg ttgctgatgg gctggcagaa atttacccaa
     8521 caacgccctc atgcgccgac cgattacatg gaagcgttgc aaaccgatgg acagttcgat
     8581 tacgcagcaa gcctggttaa atcgcttgcc tcgctgctgg tagtaaccag cggcagtgca
     8641 attggtcgcg aaggtgcgat gattctttta gctgcccttg ccgcctcctg ttttgcccaa
     8701 cgttttacgc cacgccagga gtggaaatta tggatcgcct gtggggccgc ggcgggaatg
     8761 gctgcggcct atcgtgcccc gcttgctggc agtttattta tagccgaagt gctgtttggc
     8821 actatgatgt tggcctctct cggcccggtg attatttccg ccgtcgtggc attgctggtt
     8881 agcaatctga ttaatcatag cgacgcgtta ctctacaacg tacaactctc agtgacggtt
     8941 caggctcgtg actatgcgct gattatcagt acaggtgtgc tggcaggtct gtgcggacca
     9001 ctgttgttaa cgttaatgaa cgcctgtcat cgtggatttg tgagtctcaa acttgcgccg
     9061 ccctggcaac tggcactagg cgggttgatc gtgggtctgc tttccctgtt cacacctgca
     9121 gtgtggggca acggctatag caccgtacaa tcctttttaa ccgccccacc actgttaatg
     9181 atcattgccg ggatcttcct ttgtaaactg tgtgccgtgc tggcgagtag cggttccggc
     9241 gcacccggtg gggtctttac accgacgcta tttatcggtc ttgccattgg catgttgtat
     9301 ggtcgtagcc tgggattatg gttccctgat ggcgaagaaa ttacactttt actcggattg
     9361 accgggatgg cgacactgtt ggcggcaacc acgcacgcgc cgattatgtc gacgttgatg
     9421 atatgtgaaa tgaccgggga gtatcagcta ctccccggtt tattgattgc ctgcgtaatt
     9481 gcgtcggtaa tctcgcggac gttacaccgt gactctatct accgccagca cactgcgcag
     9541 catagctaag cggatgtatt gtcccagttc acgctgttcg gcgcgcggca gataaggcag
     9601 ttcaccaatg agcggtgccg gaagtttttt accaagcaca tcaatgattt ccgcataatg
     9661 cgccagtcct ggattgattc ggttagccac ccagccaatg agtggcagcc cgtcgttggc
     9721 aatcgcctga gctgttagca gtgcatggtt aatgcaacct tcctgaatac cgacaaccat
     9781 caacaccggc agttgttcct gcaccaccc
//
LOCUS       DAEVZA010000163         9635 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEVZA010000163 DAEVZA010000000
VERSION     DAEVZA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.163"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(75..1667)
                     /locus_tag="LAF92_004379"
     CDS             complement(75..1667)
                     /locus_tag="LAF92_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBI9793296.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1886..2806
                     /gene="ldtB"
                     /locus_tag="LAF92_004380"
     CDS             1886..2806
                     /gene="ldtB"
                     /locus_tag="LAF92_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBI9793297.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2865..3983)
                     /locus_tag="LAF92_004381"
     CDS             complement(2865..3983)
                     /locus_tag="LAF92_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBI9793298.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3980..4447)
                     /gene="mntR"
                     /locus_tag="LAF92_004382"
     CDS             complement(3980..4447)
                     /gene="mntR"
                     /locus_tag="LAF92_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBI9793299.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4633..4761
                     /gene="mntS"
                     /locus_tag="LAF92_004383"
     CDS             4633..4761
                     /gene="mntS"
                     /locus_tag="LAF92_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBI9793300.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5033..6616
                     /gene="opgE"
                     /locus_tag="LAF92_004384"
     CDS             5033..6616
                     /gene="opgE"
                     /locus_tag="LAF92_004384"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBI9793301.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6665..7180)
                     /gene="ompX"
                     /locus_tag="LAF92_004385"
     CDS             complement(6665..7180)
                     /gene="ompX"
                     /locus_tag="LAF92_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBI9793302.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7233..7298)
                     /locus_tag="LAF92_004386"
     CDS             complement(7233..7298)
                     /locus_tag="LAF92_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793303.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7533..8420
                     /gene="rhtA"
                     /locus_tag="LAF92_004387"
     CDS             7533..8420
                     /gene="rhtA"
                     /locus_tag="LAF92_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBI9793304.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8719..9222
                     /gene="dps"
                     /locus_tag="LAF92_004388"
                     /gene_synonym="pexB"
     CDS             8719..9222
                     /gene="dps"
                     /locus_tag="LAF92_004388"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBI9793305.1"
                     /translation="MSTAKLVKSKATNQLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         2425 a         2511 c         2266 g         2433 t
ORIGIN      
        1 gtagcgcatc aggctatttt gcatttgtca tcagccgccg tcaccccgac agtatcgggg
       61 tgacggataa tcacttactc gatcccttta ctacgcaggt aatcttcgta attaccgcta
      121 aagtcgatca cgcgttccgg ggtgatttcc agaatgcggg tcgccaggga gcttacgaac
      181 tcacggtcgt gtgaaacaaa gatcagcgtg ccctgataca gttccagcgc catgttcagt
      241 gactcgatgg attccatatc caggtggttg gtcggttcgt ccatgatcag aatgttcggc
      301 ttctgcatca ttaacttacc aaacagcatc cgcccttttt ccccaccgga aagcacttta
      361 gctggctttt tgatgtcgtc ctggctgaac agcaaacgac cgagaatgct gcgtaccgcc
      421 tgctcgtcat cgccttcctg cttccactgg ctcatccatt cgaacacggt cagatcattt
      481 tcaaactcat attcgtggtc ctgagcatag tacccaatgc gcgcgttttc agaccatttt
      541 acggtgccgc tgtccggttg cagatcgccc accagcgttt tcagcagcgt tgatttaccg
      601 acgccgttgg tacccagtac cgcaagtttt tcaccgacct ccagcagcag gttgagattt
      661 ttaaacagtg gaccgttatc aaaccctttg gtcagacctt ccacttccag cgcgttacgg
      721 aacagtttct tatcctgttc aaaacggatg aacgggttct gacggctgga ggctttcacc
      781 tcttccagtt tgattttatc aatctggcgc gcgcgcgaag ttgcctggcg agatttcgag
      841 gcgttggcgc taaagcggct aacgaaagat tgcaactcag caatctgcgc tttcttcttg
      901 gcgttatcgg ccagcagacg ttcacgcgcc tgggtcgccg ccgtcatgta ctcatcgtag
      961 ttgcccggat aaacgcgcag ctcgccgtaa tccagatccg ccatgtgggt acagaccatg
     1021 ttaaggaagt gacggtcgtg cgagatgatg atcatcgtgc tgtcacgctc gttcagcacc
     1081 tgttccagcc agcgaatggt gtcgatgtcg aggttgttgg tcggttcgtc gagcaggaga
     1141 atatccggat cagcaaacag cgcctgcgcc agaagcacac gcagcttcca gccaggagca
     1201 acttcactca tcgggccgta gtgctgttcc actggaattc ccacgccaag caacaattca
     1261 ccggcgcgag cttccgcaga gtaaccgtcc atttcgccgt atttaacttc cagatcggcc
     1321 actttatagc cgtcttcctc actcatttcc ggcaaagcat agatgcggtc acgctcctgc
     1381 ttcacttccc acaactcttt atgccccatg atcaccgtat ccagcacagt gaactcttca
     1441 aaggcaaact gatcctgacg cagcttacca atgcgctcgt tgggatcgag ggaaacgtta
     1501 cccaaagtcg gctctaagtc gccgccgagg atcttcataa aggtggattt accactaccg
     1561 ttcgcgccaa tcaggccgta acggttgccg ccgccaaatt tgacggaaat gttttcaaac
     1621 aacggcttac tgccgaactg catggtgacg ttactggaaa ctaacacagg cgtatcctga
     1681 aaagagatat gacaaaccgc gcattatgcc ataagtctga aagaagatcc tgctcctcgc
     1741 ttaaggtcta cactcaagcc aaaggcaaaa aatgtgattt cgtacacatc tgatttcact
     1801 gtgagctgga atgaacttat aatgcgcttc caatactcta atattctcaa cccaatggcc
     1861 tgccaggcac aaaatctcgc ttaacatgaa tatgaaattg aaaacattat tcgcagcggc
     1921 cttcgctgtt gtcggctttt gcagtaccgc ctctgcggta acttatcctc tgccaaccga
     1981 cgggagtcgc ctggttggtc agaatcaggt gatcaccatt cctgaaggta acactcagcc
     2041 gctggagtat tttgccgcgg agtaccagat ggggctttcc aatatgatgg aagcgaaccc
     2101 gggtgtggat accttcctgc cgaaaggcgg tactgtactg aacattccgc agcagctgat
     2161 cctgccggat accgttcatg aaggcatcgt cattaacagt gcagagatgc gtctgtatta
     2221 ctatccgaaa gggaccaaca ccgttatcgt gctgccgatc ggcattggtc agttaggcaa
     2281 agatacgcct atcaactgga ccaccaaagt tgagcgtaag aaagcaggcc cgacctggac
     2341 gccgaccgcc aaaatgcacg cagagtaccg cgctgcgggc gaaccgcttc cggctgtcgt
     2401 tccggcaggt ccggataacc cgatgggtct gtatgcactc tacatcggtc gcctgtatgc
     2461 tatccatggc accaacgcca acttcggtat cggcctgcgt gtaagtcatg gttgtgtgcg
     2521 tctgcgtaac gaagacatca aattcctgtt cgagaaagta ccggtcggta cccgcgtaca
     2581 gtttattgat gagccggtaa aagcgaccac cgagccagac ggcagccgtt atattgaagt
     2641 ccataacccg ctgtctacca ccgaagccca gtttgaaggt caggaaattg tgccaattac
     2701 cctgacgaag agcgtgcaaa cagtgaccgg tcagccagat gttgaccagg ttgttcttga
     2761 tgaagcgatt aaaaaccgct ccgggatgcc ggttcgtctg aattaatctt caaaacttaa
     2821 agcaaaaggc ggactcataa tccgcctttt ttatttgcca gaccctagtt ggccgggagt
     2881 ataactaaca aaacatatcc caccaacgcc gcccacaaca gcatcggtat tgaatagaga
     2941 tggaaacgcc accagatgcg gcgatcgttc gccatacgta gcgcaatcaa atttgccagc
     3001 gatccgggta ataacccaaa gccacctacg tttaccgccc ataccagtaa taaagacggc
     3061 ggcacatagt tcagcaacaa tatggtactc ggcacgttac tgatcacctg cgataaaccg
     3121 attgccgtta accataaccc gggttcagat agatgactca cgttacccaa cacgccttgc
     3181 aacgctggaa gctgggtcag taaatggacg tcgataaaca tcgccataaa caccagcagc
     3241 agcgtccagt ccacactgag caccacgcga cgtgccagca gcgcaaagcc tgccgccaca
     3301 atcaccagtc cccacagctc ttgtttgaac tccagcgccg tcagaaagac gatatacagc
     3361 cccagacaac tccacaccag ccgcggtttc cactccggtg tttgcacccc cgtatggtat
     3421 tgcagcgcct ttccagggaa acaacaccag cacaggagca ggagcgtcag catcattgcg
     3481 ccagccagcg gtgccatttg ggcaataaat ccggcaaacg aaagaccaga acgtccccag
     3541 ataagaatat tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt gactgccagc
     3601 gcctcaaaaa taatcagccg attaaccggg atctcacaca gtctttttag cgtgatagtc
     3661 agcggaacaa caataaacag cgcgacatcg ttggtcagaa aggtagaaag cagcgccgcc
     3721 gccagcacca taaacatcgc cagccgacgc tccgtagcaa agcggcgcac cattttgcgc
     3781 cccagcacat caaaataacc gcttaactcc acacctttgg tcagcagcat caggccgctt
     3841 aaggtgatga tggtgtgcca gtcgatagca gcaggccagg atttcggtgc aaagggcacg
     3901 aaaaagctta atccgatacc aacaagaatt aataactgaa aaaaacgatc gccttgcagc
     3961 gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg ggtaaacaaa cgaaaagcgt
     4021 cgagcgtctc ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg
     4081 gactgacgcc caacaccaac aagaaatttt cgactatctg atgtcgctcg cggctttcct
     4141 gcgccagctt ctctccttct gccgttaaaa acacgccacg ccaggggatc atttcaatca
     4201 gccccatggt tgccagccgc ttaagcattt tagccaccgt cggttgcgaa actcccagac
     4261 gagcagccat gtccacctga cgagcttccc ccacttccct gatcaagtca gaaatcagct
     4321 caacgtaatc atcaataagc tcgcgccgat gcgcctctct gacctggcgg aacccttcaa
     4381 cgtgctcttc cacgttcact aactgcgtca ctttttttgc tgttggcgta cctgcgcgac
     4441 gactcattgt gcttcctcat tatggtgacg catacatagc gttctcattt cgtgcgcaca
     4501 ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc tatataatat
     4561 agcctgtgct atatctgtat gtaatgcaat catccctcaa ggatcgacgg gattagcaag
     4621 tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca ttctcccttt
     4681 aaagtacggt taatgctgct ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa
     4741 gataaacctt ccgataaata gcggcgtcgc ggtacgccgc ttcactcctg ctttcatgca
     4801 ggcataacgc gttttggtct gaaaaacccc actttttgtc ggatttgcaa tccccttcgc
     4861 aaaagatttg ttcgtcagta gttgacctga acggcggctc gctctatctt cttgcagccc
     4921 tgcgtatatt gcggctcgcg gatgcggacc cctttccact cttcacgcac tcttgcaggt
     4981 attgaccctt gacgccaggg taagcacatg gcgtttgtta cgatagtggc atatgaattt
     5041 aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt agcccgtgga ctgcgttcta
     5101 ctttttacag tcgctattaa ttaacctcgg cttaggttac cccttcagtt tgctctacac
     5161 cgctgcgttt acggctattt tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt
     5221 tctggtcggt gtcagttcgc tggtggcggc ttgttatttc ccttttgctc aggcctacgg
     5281 cgcgcctaat ttcaatacat tgctggcatt gcactccacc aatatggaag agtcgaccga
     5341 aatcctgacg atttttccgt ggtacagcta cctggtcggc ttatttattt ttgcgctcgg
     5401 cgtaatagca atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata ccttcgacag
     5461 cctgtgtctg gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa acctggcctg
     5521 gggtggcgta tttaaactga aagatactgg ctatccggta tttcgttttg ctaaggatgt
     5581 catcgtcaat aataacgagg tgattgaaga gcaagaacgg atggcaaaac tttccggaat
     5641 gaaagatacc tggacggtca ctgccgttaa gccgaagtat cagacctatg tggtggtgat
     5701 cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg acaatacccc
     5761 gtttgccagc agcgttaacg gtttgatatt tgctgactac attgccgcca gtggctccac
     5821 gcagaaatcg cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac agtttcagga
     5881 taactttgtc accctggcaa atcgcgcggg cttccagacc tggtggtttt ccaaccaggg
     5941 tcaaatcggc gaatacgata ccgctatcgc cagcatcgcc aaacgagcag atgaagtgta
     6001 cttcctgaaa gaaggtaatt ttgaagcaga taaaaatacc aaagacgaag cgttactgga
     6061 tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg cagctgattg ttctacatct
     6121 gatgggctca catccgcagg cctgcgacag gacacaaggc aaatacgaaa cctttgtgca
     6181 gtcgaaagaa acgtcgtgct atctctatac catgacgcaa acggacgatt tactgcgcaa
     6241 gctgtacgat cagttacgca acagcggcag cagcttctcg ctggtttact tttctgacca
     6301 cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg atgataagta
     6361 tcagcaaaat ttccaggtgc cttttatggt catttccagc gacgataaag cgcatcgggt
     6421 gattaaagcc cgccgctcag ccaatgactt cttaggtttt ttctcccagt ggacgggaat
     6481 taaagcgaag gaaattaaca tcaaataccc gtttatatct gagaagaaag ccgggtcgat
     6541 atacatcacc aacttccagt tacagaaggt ggattacaac catctcggaa ccgatatttt
     6601 cgacccgaag ccttaaaaac aaaaatccgc cccgagaggc ggatttttta tatcaccaaa
     6661 gtgattagaa gcggtaacca acaccggcaa tccaggtgcc tacgtcaacg ctacgaatac
     6721 ggctctgctc gtaagagaag tccagagcaa cgttttccat cgggttgaac tgcagaccag
     6781 caccgtagga gaaaccgtag tcgctggtgt cgtgtttgta ggtcgggtat tcagtggtct
     6841 ggaatttacc ataacccaca cccactacac cgtagatgct tgcccagtcg ttaatgcggt
     6901 aagccggacc agcagtgatg ccgtagtact ggtttttgtt gtagtcacca gagcttgcag
     6961 tacggctttt ctcggtgtaa gtgaaagaac cgatcacacc cagcgggctg ttgtcttctt
     7021 catagcggta tttcaggttg aaaccgccca ttttgttcat ttggccctga gcgtcgctct
     7081 gtgcgtaacc gccagttacg gtagaagtcg cagctacgga agtacctgcg gtgaaagcca
     7141 gaactgcggc cagtgctgaa agacatgcaa tttttttcat aaccacctca aatgtgattc
     7201 aaataagtcc taagttttaa atatatcaaa aattaatggg aaactcttcg cgatttgtga
     7261 tgtctaacgg gccatttcat gtaacagaac gtttccatac accgctatcc atctaaattt
     7321 aaatcacttt ttcagagaac tgcgtaagta ttacgcatgt tttccctgtc attcatccag
     7381 attattccta atcaccagac taatgattcc atcaatcctg gcgcatttta gtcaaaacgg
     7441 gggaaaattt tttcaacaaa tgctcaacca gcattgggta tatccagtac actccacgct
     7501 ttacttaagt ctagatattt gtgggagaaa ggatgcctgg ttcattacgt aaaatgccgg
     7561 tctggttacc aataatcata ttgctcgttg ccatggcgtc tattcagggt ggagcctcgt
     7621 tagctaagtc actttttcct ctggtgggcg caccgggtgt cactgcgctg cgtctggcat
     7681 taggaacgct gatcctcatc gcgttcttta agccatggcg actgcgcttt gccaaagagc
     7741 aacggttacc gctgttgttt tacggcgttt cgctgggtgg gatgaattat cttttttatc
     7801 tttctattca gacagtaccg ctgggtattg cggtggcgct ggagttcacc ggaccactgg
     7861 cggtggcgct gttctcttct cgtcgcccgg tagatttcgt ctgggttgtg ctggcggttc
     7921 ttggtctgtg gttcctgcta ccgctggggc aagacgtttc ccatgtcgat ttaaccggct
     7981 gtgcgctggc actgggggcc ggggcttgtt gggctattta cattttaagt gggcaacgcg
     8041 caggagcgga acatggccct gcgacggtgg caattggttc gttgattgca gcgttaattt
     8101 tcgtgccaat tggagcgctt caggctggtg aagcactctg gcactggtcg gttattccat
     8161 tgggtctggc tgtcgctatt ctctcgaccg ctctgcctta ttcgctggaa atgattgccc
     8221 tcacccgttt gccaacacgg acatttggta cgctgatgag catggaaccg gcgctggctg
     8281 ccgtttccgg gatgattttc ctcggagaaa cactgacacc catacagctg ctggcgctcg
     8341 gcgctatcat cgccgcttca atggggtcta cgctgacagt acgcaaagag agcaaaataa
     8401 aagaattaga cattaattaa atttacattt ctgcatggtt atgcataacc atgcagaatt
     8461 tctcgctact tttcctctac accgtcttta tatatcgaat tatgcaaaag catatttatt
     8521 ccgaaaattc ctggcgagca gataaataag aattgttctt atcaatatat ctaactcatt
     8581 gaatctttat tagttttgtt tttcacgctt gttaccacta ttagtgtgat aggaacagcc
     8641 agaatagcgg aacacatagc cggtgctata cttagtctcg ttaattactg ggacataaca
     8701 tcaagaggat atgaaattat gagtaccgct aaattagtta aatcaaaagc gaccaatcag
     8761 ctttataccc gcaacgatgt ctccgacagc gagaaaaaag caacagtaga gttgctgaat
     8821 cgccaggtta tccagtttat tgatctttct ttgattacca aacaagcgca ctggaacatg
     8881 cgcggcgcta acttcattgc cgtacatgaa atgctggatg gcttccgcac cgcactgatc
     8941 gatcatctgg ataccatggc agaacgtgca gtgcagctgg gcggtgtagc tctggggacc
     9001 actcaagtta tcaacagcaa aaccccgctg aaaagttacc cgctggacat ccacaacgtt
     9061 caggatcacc tgaaagaact ggctgaccgt tacgcaatcg tcgctaatga cgtacgcaaa
     9121 gcgattggcg aagcgaaaga tgacgacacc gcagatatcc tgaccgccgc gtctcgcgac
     9181 ctggataaat tcctgtggtt tatcgagtct aacatcgaat aaatccatcg ctgatggtgc
     9241 agaactttag tacccgataa aagcggcttc ctgacaggag gccgttttgt tttgcagccc
     9301 acctcaacgc acttatttag tgcatccatc tgctatctcc agctgattaa gtaaattttt
     9361 tgtatccaca tcatcacaca atcgttacat aaagattgtt ttttcatcag gttttacgct
     9421 aaataatcac tgtgttgagt gcacaatttt agcgcaccag attggtgccc cagaatggtg
     9481 catcttcagg gtattgccct ataaatcgtg catcacgttt ttgccgcatc tcgaaaaatc
     9541 aatgagttgc aaaactggca cgattttttc atatatgtga atgtcacgca ggggatcgtc
     9601 ccgtggatag aaaaaaggaa atgctatgaa gtctg
//
LOCUS       DAEVZA010000164         9566 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEVZA010000164 DAEVZA010000000
VERSION     DAEVZA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.164"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1..978
                     /locus_tag="LAF92_004389"
     CDS             1..978
                     /locus_tag="LAF92_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9793306.1"
                     /translation="MPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNAPGFISLN
                     NQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEVPLAIACL
                     LVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIDENEVLDW
                     LGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGINVRRVRIL
                     IFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRGSLFGTLL
                     GVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            1011..2105
                     /locus_tag="LAF92_004390"
     CDS             1011..2105
                     /locus_tag="LAF92_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBI9793307.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDKVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            2117..2989
                     /locus_tag="LAF92_004391"
     CDS             2117..2989
                     /locus_tag="LAF92_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBI9793308.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLLALAPG
                     ESTTSEMSLRVALL"
     gene            complement(3037..3459)
                     /locus_tag="LAF92_004392"
     CDS             complement(3037..3459)
                     /locus_tag="LAF92_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBI9793309.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(3556..4758)
                     /locus_tag="LAF92_004393"
     CDS             complement(3556..4758)
                     /locus_tag="LAF92_004393"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBI9793310.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(4768..5580)
                     /gene="hcaB"
                     /locus_tag="LAF92_004394"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(4768..5580)
                     /gene="hcaB"
                     /locus_tag="LAF92_004394"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBI9793311.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(5577..5897)
                     /locus_tag="LAF92_004395"
     CDS             complement(5577..5897)
                     /locus_tag="LAF92_004395"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBI9793312.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(5897..6415)
                     /locus_tag="LAF92_004396"
     CDS             complement(5897..6415)
                     /locus_tag="LAF92_004396"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBI9793313.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(6412..7773)
                     /locus_tag="LAF92_004397"
     CDS             complement(6412..7773)
                     /locus_tag="LAF92_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBI9793314.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            7909..8799
                     /gene="hcaR"
                     /locus_tag="LAF92_004398"
     CDS             7909..8799
                     /gene="hcaR"
                     /locus_tag="LAF92_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBI9793315.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            8959..>9566
                     /locus_tag="LAF92_004399"
     CDS             8959..>9566
                     /locus_tag="LAF92_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBI9793316.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVR"
BASE COUNT         2095 a         2478 c         2649 g         2344 t
ORIGIN      
        1 ttgccgcagg gcaagagcgt ctcgctcaaa caatttgtca gtcgccatat taatgagatc
       61 ggtttgctgg tggtgattgc cattctttat ctggtcttct ccctgaacgc gcctggcttt
      121 atctcattga ataaccagat gaacgtgctg cgcgatgccg ccaccattgg gattgccgcc
      181 tgggcgatga cgctgattat tatctccggt gaaattgatg tcagcgttgg gccgatggtg
      241 gcttttgtct cggtgtgcct ggcatttttg ctgcaatttg aagttccgtt ggcgattgct
      301 tgtctgctgg tgttgctgtt aggcgcgctg atgggaacgc tcgccggggt gctgcgcggc
      361 gtgtttaacg taccaagttt cgttgccaca ctggggctgt ggagcgccct gcgcggaatg
      421 gggctgttta tgaccaacgc actgccagtg ccgattgacg aaaacgaggt gctggactgg
      481 ctgggcggac aatttctcgg tgtgccagta tccgcgctga tcatgattgt gttgtttgcg
      541 ctgtttgtgt tcatcagccg caaaaccgcc ttcgggcgct cggtttttgc cgttggcggt
      601 aatgctacgg cggcgcagtt gtgcggcatc aacgttcgtc gggtgcgcat tcttatcttt
      661 accctttcgg gattattagc ggcggtgacc ggcattttgt tggcggcgcg cctcggttct
      721 ggtaacgcag gtgccgcaaa cggtctggag tttgacgtca tcgccgcggt cgtggtcggc
      781 ggtacggcac tttccggcgg tcgcggttcc ctgttcggta cattgcttgg tgtgctggtg
      841 attacgctaa tcggtaacgg tctggtgctg ctcggtatta actccttttt ccagcaggtg
      901 gtgcgcggcg tcatcatcgt ggtggcggtg ctggcgaata tcttgctgac ccagcgtagc
      961 agtaaagcga aacgctaaac cttaacgccc ctggcgcggg tgcggcaggg atgaaaatta
     1021 atcgtattgt taatgaggga tttatgaaaa cgatgctggc agcttattta ccaggaaatt
     1081 cgaccgtcga tctgcgggaa gttgcggtgc cgacgccggg gattaaccag gtactgatca
     1141 aaatgaaatc ctccgggatt tgcggaagcg atgtccacta tatctatcat caacaccgtg
     1201 ccacggcggc ggcacccgat aaaccgttat accagggatt tatcaacggt catgaaccgt
     1261 gcgggcagat tgtggcgatg gggcaaggct gtcgccattt taaagagggt gatcgcgtgc
     1321 tggtgtatca catttctggc tgtggttttt gcccgaactg ccgccgcggc tttcctatct
     1381 cttgtactgg caaaggaaaa gcggcttacg gctggcagcg tgacggcggt catgccgaat
     1441 atctgctggc ggaagaaaaa gatctgatcc tcctgccgga tgcgctgagc tacgaagatg
     1501 gtgcgtttat cagttgcggc gttggcacgg cctatgaagg gattttgcgc ggcgaagttt
     1561 ccggcagcga taaagtgctg gtggtcggtc tggggccggt cggcatgatg gcgatgatgc
     1621 tggcgaaagg tcgcggtgca aaacggatca tcggcgttga tatgctgccg gaacgtctgg
     1681 cgatggcaaa acagttaggg gtgatggatc acggctattt agcaaccacc gaaggtctgc
     1741 cgcagattat cgccgaactc acccacggtg gcgcggatgt tgcgctcgat tgttccggta
     1801 atgccgcagg tcgcttgctg gcactgcaat ccaccgctga ctggggacgg gtggtttaca
     1861 ttggtgaaac cggaaaagtg gaattcgagg tcagcgccga tctgatgcac catcaacggc
     1921 ggattatcgg ctcctgggtg accagtctgt tccatatgga aaaatgcgcc catgatctga
     1981 cggactggaa gctgtggccg cgtaacgcca ttacccatcg cttctcgctg gaacaggcag
     2041 gtgatgccta tgcgctgatg gcgagcggca aatgcgggaa agttgtgatt aacttcccgg
     2101 attaaggagt tagcggatga ccatctatac cttatcccac gggtcgctaa agctggacgt
     2161 ttctgaccag ggcggcgtaa tcgaaggctt ctggcgcgat acgacgccgc tcttacgtcc
     2221 tggtaaaaaa agcggtgtgg caaccgatgc ctcatgtttt ccgctggtgc catttgcaaa
     2281 ccgggtgagc ggcaatcgtt ttgtctggca ggggcgtgag tatcaactgc aaccgaatgt
     2341 tgagtgggat gcgcactatc tacacggcga tggctggctg ggcgagtggc aatgtgtctc
     2401 gcacagcgat gatagtttgt gtctggtgta tgaacatcgc agcggtgtct atcactatcg
     2461 ggtaagtcag gcgtttcatt taacggcgga tacgctgacg gtgacgctct ctgtcaccaa
     2521 tcaaggggca gagacgctgc catttggtac cggctggcat ccttattttc cgttgtcgcc
     2581 gcaaacgcgg attcaggcac aggcgagcgg ttactggctg gagcgggagc agtggctggc
     2641 gggtgagttt tgcgagcagc taccgcagga actggatttt aaccagcccg cgccgttgcc
     2701 gcgccagtgg gtgaacaatg gttttgccgg atggaatggt caggcccgca tcgagcagcc
     2761 gcaggaaggt tatgccatca tcatggaaac gacgccacct gcaccgtgtt atttcatctt
     2821 tgtttccgac cctgcgtttg ataaaggata tgcgtttgat ttcttctgtc tggaaccgat
     2881 gagccatgcg ccggatgatc atcatcgccc ggaagggggt gatcttcttg cgcttgcacc
     2941 aggtgaatca acaacttcag agatgtcgtt gcgggttgcg ttgctgtaat ttattaaatg
     3001 ccggataagg cgttcacgcc gcatccggca tctttcctaa cgccgatcga gagaaattgc
     3061 ccccggcccg gtaattgcca gcagcaggaa tgcgccagcg atactgacat tcttccagaa
     3121 attaatcata tttggcccaa ccgcatcgcc ggtcatatcc cagtaatggt gaccaatcac
     3181 cgccgtaccc agcgtgtaga aaataaacag caccgccagc ggacgggtga aaaagccaag
     3241 cacgattaat atcgcggcgg gcacttccat aactaccgca ataatcgctg ccagcatcgg
     3301 cattggcgcg cccaacgagg ccatatattg gaccgtaccg tcaaagccca tcattttggg
     3361 aaaaccaaaa ataatgaaaa ttaagaccac ggcgatacgg gcaattaata acaaaacggg
     3421 gcgggcagct ccaaaatcaa aataacgtag tgtgttcata accaatccct ttgcaagata
     3481 atgtgattta aacgtaatac acactcttgc acctcgccac gccccaggca ttgagatatt
     3541 cgctaattat cctggttaca gtgatttaag cgcgatgttc tcatctatca gcagtttcgc
     3601 atcaaacgtt ttgccgctct ggatccattt gcgaattggg cgaatctcac gcccctgatt
     3661 cagcgtgacc gcaccgataa gcacgccgtt ttgcagatta aaccaaatcg ccttctgagt
     3721 ttccgggttg ccacgacaaa gccagtcatc gccacgcata tcgccaataa actgtaagtt
     3781 atcactgtac tgatcgctcc agaaccacgg cggcggcagt agcggtagcg gtagccctaa
     3841 cattgcggca gcggcaattt gcgcctggtt attggcgttt tcccagcttt cgcagcggtg
     3901 tagtgcacca ttatcaagac gagtgattgc cacatcgcca ccggcaaaga tcgcgggatc
     3961 gcaggtgcgg caagcctcat caatgacaat gccattggca gtatcaaggt tggcctcgcg
     4021 agccagttgc tcgttggcgc tgataccaat accgtaaatc accacatcag cctgaagcgt
     4081 ttccccactt tgcagcgtca gttctacttt ttcaccatcg accacatgtt caatggcatt
     4141 attgagcaga atgcgcacac cagcctgctg gtggcgctgt aaaagatagc gttgcacggg
     4201 cggtggtgca ttacggccca tgacggttgc cgccagttca atcactgtca ccttacatct
     4261 gcgctgcgtg gcgctggcag ccagttccag accaatagtt ccggcaccga caatcacgac
     4321 tgaccgttcg ggctgcagaa cttctcgcag tctggcggca tcaccggcat ggcgtagggt
     4381 aaagcagcgt tctcccagtg catcaagcaa cggcagcggt cgagctgccg cgccggttgc
     4441 tataaaaagc tgatcccagt gccagctttc gccgttggtt aacactaact ctcgtgtgtc
     4501 gcgacccagc gttttgatgg ttacaccgga atgcagatga acattgtttt cctgccacca
     4561 gttagcgggt aacacctgct gtaactgcgg ggaatcttcc agcaacatgg atttcgagag
     4621 tggaggtcgt tcataaggaa gatgtcgctc atcggaaaac agatgcagct caccggtgaa
     4681 cccttgctgg cgtagcgagg ccgcagccat tgccgccgct tgcccgccac cgacaataat
     4741 gatcgttttt tctttcatcg tgcttcctta aagatccagc ccagccgcta cgtggcgaat
     4801 gccgcgaatc gctaaacccg catcagcgtt gatcatcaca ccgcttaatg cgcgattatt
     4861 gcgccgcgat gccaacatca catacggccc cgtaaaatcc gccggttgcg ggaaaaattg
     4921 cagcggtaaa atggcggcaa ttttctccgg cgtcagagac tgcattatcg aggtttcact
     4981 ttgcccgagc gcctgtgggc cgcgcaggtc gctggccata ccacacgggc cgacgccatt
     5041 cacccgcact ttcggtgcca gttcataagc cagttggcga ataagtccgg ttgcggcatg
     5101 tttactggcg gtgtacagcg ggccaccgcc gccaggatac caggcggcat ttgacagtgt
     5161 gaaaatcatg ctgccttcac tggcgattaa cgccggagcg caggcttttg cgcccagcag
     5221 gtaaccgaga acattgacgt taaacagctc gtggaagccg gtttcgagcg tctctgcggg
     5281 agtattaacc agtgaggcat tgtgatccca gatgcctgca ttgccgataa aacaatccag
     5341 cttgccggaa cgagtcagga tctgatcgac cgcgcgttga taatcggcat aacaggtcac
     5401 gttaccttcc accgccagaa tatgttcgcc aaatcgctga cgcagactgg cgacttttgc
     5461 cgccgacagt tccagcgtgg caacctgcgc gccttcttcg ataaatcgct cgaccagcgc
     5521 cagccctaat cccgatccgc cgccggtaat aaaaatggac tcgttatgca gatcgctcat
     5581 ggctgcgcct ccggtaagtc gatgaaaatg tcaccacctt caacgtgtac tggataagtg
     5641 gtgagcggat cggtggcggg caggcataac gctttccccg ttttcaggca aaaactggcg
     5701 gcgtgtagcg ggcactccac cgtggcgtca tcttccagat acccttctga cattgacgca
     5761 ttaccatggc tgcaacgatc gttaatggca taaaactcgc cgccaacgtt gaacagggcg
     5821 atgacgggcg aggtatcgat ccggagagcc tcaccctccg gcacatccgc aacgggacac
     5881 gcataaattc gattcatcag aacagtacac tcaggttatg ggaagtgatt accgcttgat
     5941 ccaggacgat atcccgttcc agcaagcgcc agttgtcatc ttccagacgg cgaactttgt
     6001 caaaacgagt cccgacatag aacgtttcat cgcgctcttt ttgtgcccga taaagcagat
     6061 aattcacccg aacagcaaat acgtttggga tgtcggtttc gcttatctgg cagttgctga
     6121 ttaagtggcg ggtgcgtgac ggcggctctt ctgcccatgc catgcccgtt tccagacggg
     6181 cgattcgccg ctccagctgg tctttggtgt cattaaaaat ccaggttgtc ggtggctgga
     6241 cgcctttgcg gcggtcgcgt gtttgcgcgt taactgtggt gcgcatggtg taacgaatct
     6301 cttcgtcgag ctgcgccagc cagtcacgaa atttccagtc gtccagtaag ctggcttcgt
     6361 gaaagagaaa ctggctaatg cggtgatgta actctagtga aacttgcgca ctcatttcat
     6421 cacctcctgc tggtaagcag cggttttatc gagcacttcc tgccagcttt cgctactcag
     6481 aagatcggcc cagcgttggt acattccacg agcggcagtt tctgaaaaga tatagttagt
     6541 aatgccagga atgccgtcgt cgcggcgctt ttcctgacca agccccattt ccagacacag
     6601 tttgctgttg cgggcgcggt ggcctttaag caatttctgg atttcacacc agttctccga
     6661 gtcatcctgc tcgagaaaac cagcaggacc aaaagcacga gtggcgctgt tttcaaaagc
     6721 ggctttaact tcatcggagg cggctttgtc agtaatacag aacgcccaca cttcaacttg
     6781 atcagggccg cgcggatgcc agacgcggag tgtggcagtg ccgtttagcc atgaaagcgt
     6841 ggggaatata ttgttatgac ccgccaggcg cagggcgcga acttcaccta aacgttgttc
     6901 tgcttcggca taggtttcgc ggtaatagct tgaaactgcg ccatcgaccc agacattagc
     6961 atccggtttt tcagtaaaga agaaaccgct accgtgaccg tcctgaccaa attgcagcgc
     7021 atctttggcg gtttcccaca ccgggcgggc ggtttgtcca tcaccgaggc gcttatcgct
     7081 gccatcatct ttcgccccta atacctgaac ggcagaagca tggctgaaca gagcatgata
     7141 ctggtcactg gcgaactgct ctgccgggaa tttccagtta cagttgatca cccacttttg
     7201 tacgccgccg acaatttcgg tgccgccttc gcgacgatcc agcatgccat ccagatacca
     7261 ggcaatgtca cccaggtaat cacgcaggcc cggtgcgctg gtatcccagt tgccaaaaat
     7321 cagcccttta taactctcca cacaaggaac ttcgtttagt ccccagtggg atttacacaa
     7381 cccttgtggg taggcgcgag gttccagcgg tacatcgatc aactcgccgt taatgccata
     7441 agaccagccg tgatacgggc aggtaaaggc gcgagtgttg ccgcaatctg cataactcac
     7501 acgcatggcc cggtggcggc attggttgag aaacgccttg atgctgccgt ctttctgacg
     7561 cactacgaca accgcatctt cccccatgta ggtgttaaag aaatcaccag gttttgggat
     7621 ctggctttcg tgggcgagaa atagccagca acggccgaaa atacgttcaa gctccagttg
     7681 gtaaatatct gggtcggtat aaatacgcgg agtgacccga ccattttggg tatcaatcag
     7741 ttggtaaatg ttcaaatctg agggtgtggt catatttttt aatcctgaaa gataagatgt
     7801 attgaccttc tatttttgtg ccataaggat gttgctggtt ggttgctaat atgtgaaata
     7861 gttaatttct accagctgag acgttttttc gataacaagg ggaaggtgat ggaactacgg
     7921 catttacgct atttcgtcgc agtggcgcag gcactgaact ttacccgtgc ggcggaaaaa
     7981 ctgcatacct cacagccttc gttaagcagc cagatccgcg atcttgaaaa ctgtgtcggt
     8041 gttccgttac tggtgaggga taagcgcaaa gtcgcgttga cggcggcggg agaatgtttt
     8101 ctccaggacg cgctggcaat cctcgaacaa gcggaaaatg ccaaattacg ggcgcggaaa
     8161 attgttcagg aagacagaca attaaccatt ggctttgtgc catcggcgga agtgaattta
     8221 ctgccaaaag tattaccgat gtttcgtctc agacagccag acaccttgat tgagctggtg
     8281 agtttaatca ccacgcaaca ggaggaaaaa attcgccgtg gtgaactcga cgtcggctta
     8341 atgcgccatc ctgtttatag cccggagatt gattatctgg agctttttga cgaaccatta
     8401 gtggttgtgt taccggttga tcacccttta gcacatgaaa aagagatcac cgccgcgcaa
     8461 ctggatggcg tgaatttcgt cagtaccgat ccggcgtatt ccggttcgct tgcgccgatc
     8521 gttaaagcgt ggtttgcgca agaaaacagc cagccaaata tcgtccaggt ggcaacgaat
     8581 attctggtga ccatgaatct ggtgggcatg gggctgggcg tcactttgat acccggttat
     8641 atgaataatt ttaataccgg acaggttgtt tttcgtccca ttgccggaaa tgtgccttcc
     8701 attgctttac tgatggcgtg gaagaaaggg gagatgaagc cggcattacg cgatttcatc
     8761 gccattgtgc aggaacgttt ggcaaacgta acggcataat tacgcattat ttttgcaaca
     8821 tgcgtcgcgg atcgcagttt tttagtgctt gtgaactggc gttacagaca tcgggaagca
     8881 ggctcgaata ttcttatttt tcaacttgca ccaacattcg attaacccca gactaacgcc
     8941 tcctgacggg agggactcat ggttttgcaa tccacgcgct ggttggcgct cggctatttc
     9001 acatactttt ttagttacgg catttttcta cctttctgga gcgtctggct taaagggatt
     9061 ggtttaacgc cagaaaccat cggcctgtta ttgggggcag gtctggttgc ccgttttctc
     9121 gggagtttgc tcatcgcgcc ccgcgtcagc gatccttccc gcctgatttc cgccttgcgc
     9181 gtgctggcac tgctgacact tctctttgct gtcgccttct gggcgggggc gcacgtagcg
     9241 tggctgatgc tggtgatgat tggctttaac ctctttttct caccgctggt accgttgacc
     9301 gatgcactgg cgaatacgtg gcaaaagcag ttcccgcttg attacggcaa agtgcgactg
     9361 tggggctcgg tggcgtttgt cattggctcg gcgctgacgg gcaaactggt cactatgttt
     9421 gattatcggg tgatcctcgc gctgttgacg ttgggcgtgg catccatgct gctcggcttt
     9481 ctcatccgtc cgacgattca gccacaaggg gcaagccgcc agcaggagag caccggttgg
     9541 tcagcgtggt tggcgctggt tcgcca
//
LOCUS       DAEVZA010000165         9540 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEVZA010000165 DAEVZA010000000
VERSION     DAEVZA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.165"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1552
                     /gene="hrpB"
                     /locus_tag="LAF92_004400"
     CDS             <1..1552
                     /gene="hrpB"
                     /locus_tag="LAF92_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBI9793317.1"
                     /translation="RVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLHLIA
                     KEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPAVNLLAAKRLLQM
                     LGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMGNSD
                     LGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDGRYQ
                     LANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQLVQQ
                     SDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIREKGLSVLNWT
                     AEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLDIYQ
                     ALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATNPTI
                     AQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPANTAP
                     TRRTKKYS"
     gene            1748..4282
                     /gene="mrcB"
                     /locus_tag="LAF92_004401"
     CDS             1748..4282
                     /gene="mrcB"
                     /locus_tag="LAF92_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBI9793318.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            4502..6745
                     /gene="fhuA"
                     /locus_tag="LAF92_004402"
     CDS             4502..6745
                     /gene="fhuA"
                     /locus_tag="LAF92_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBI9793319.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            6796..7593
                     /gene="fhuC"
                     /locus_tag="LAF92_004403"
     CDS             6796..7593
                     /gene="fhuC"
                     /locus_tag="LAF92_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBI9793320.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            7593..8483
                     /gene="fhuD"
                     /locus_tag="LAF92_004404"
     CDS             7593..8483
                     /gene="fhuD"
                     /locus_tag="LAF92_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBI9793321.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            8480..>9540
                     /locus_tag="LAF92_004405"
     CDS             8480..>9540
                     /locus_tag="LAF92_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBI9793322.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAG"
BASE COUNT         2114 a         2423 c         2788 g         2215 t
ORIGIN      
        1 gcgtgtggcg cgttttgatc cgcgcacggg gcttacgcga ctgattactc aacgcgttag
       61 ccaggcatcc atgacgcagc gtgccgggcg cgccgggcgt ctggagccgg gtatcagcct
      121 gcatttaatc gccaaagaac aagcagaacg cgccgcggcg caaagtgaac cggagatctt
      181 acaaagcgat ctttccggtt tgctgatgga attactgcaa tggggatgca gcgatccggc
      241 gcagatgagc tggctggatc aaccgccagc ggtgaatcta ctggccgcga aacgcctgtt
      301 acaaatgctg ggggcgctgg agggtgaacg gcttagtgcg caggggcaaa aaatggcagc
      361 gctgggtaac gatccgcgtt tagcggcaat gctggttagc gcgaagaacg acgacgaagc
      421 tgctaccgcg gcaaaaattg ccgccattct cgaagagccg ccacggatgg gcaatagtga
      481 cctgggcgtg gcgttttcgc gcaatcaacc agcctggcag caacgtagtc agcaactgtt
      541 aaaacgctta aacgtacgtg gcggtgaggc agacagttcg cttatcgcgc cgctacttgc
      601 cggggcgttt gccgatcgca ttgctcgtcg ccgtgggcaa gatggacgct atcaactggc
      661 aaacggcatg ggagcgatgc tcgatgccaa cgacgcgcta agccgccacg aatggttgat
      721 cgcaccgtta ttattgcagg gcagcgcctc gccggatgcg cggattttac tggcgttacc
      781 ggtcgatatt gatgaattag tacaacgctg tccgcagctg gtgcagcaat ctgacaccgt
      841 ggaatgggat gacgcgcaag gtacgctgaa agcctggcgt cggctgcaaa tcggtcagtt
      901 gacggtgaaa gtgcagccac tggcgaaacc gtcagaagac gagttgcatc aggcgatgct
      961 taacggcatt cgtgaaaaag gtttaagcgt gctcaactgg acggcggaag cggaacagct
     1021 acgcttgcgt ttgttatgcg ccgcaaagtg gttgccggaa tatgactggc cagcggttga
     1081 tgatgaaagt ttattggcga cgctggaaac gtggctgctg ccacatatga ctggcgtaca
     1141 ttcactacgc ggcctgaaat cactcgacat ttatcaggca ctacgcggat tacttgattg
     1201 gggaatgcag caacgtctgg atagtgaatt gcctgcgcat tacactgtgc cgacgggaag
     1261 ccggatcgcc attcgttatc atgaagataa cccgcccgcg ctggcggtga gaatgcaaga
     1321 gatgtttggc gaggccacca atccgacgat cgcccagggg cgcgtgccgc tggtgctgga
     1381 gttgctttca cctgcccaaa ggccattaca aatcacacga gatttgagcg ccttctggaa
     1441 aggagcgtac cgtgaggtgc aaaaagagat gaaagggcgt tatcccaaac atgtctggcc
     1501 ggacgacccg gcaaatactg caccgacgcg acggacgaaa aagtattcgt aaggttggtc
     1561 ttctccctct ccctgtggga gagggccggg gtgagggcat cagcgcgcac gttcaccctc
     1621 accctaaccc tctccctcaa gggagagggg accgatcgag cacaaatttt gagagatatc
     1681 ttcttctgtc ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa tattgcggag
     1741 aaaaagcatg gccgggaatg accgcgagcc aattggacgc aaagggaaac cgacgcgtcc
     1801 ggtcaaacaa aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg acgattatga
     1861 tgactatgag gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca aagggcgtaa
     1921 gcctcgtggc aaacgcggct ggctatggct actgctaaaa ctggctatcg tttttgccgt
     1981 gctgatcgcc atttacggcg tttatctcga tcaaaaaatt cgtagccgta ttgatggcaa
     2041 ggtctggcaa ctgcctgcgg cagtttatgg ccgaatggtc aatcttgagc cagacatgac
     2101 catcagcaag aacgagatgg tgaagctgct ggaggcgacc cagtatcgtc aggtgtcgaa
     2161 aatgacccgt cctggcgaat ttaccgtgca ggccaacagc attgagatga ttcgccgtcc
     2221 gtttgatttc ccggacagta aagaaggaca ggtgcgcgcg cgtctgacct ttgatggcga
     2281 tcatctggcg acgatcgtca atatggagaa caaccgtcag ttcggtttct tccgtcttga
     2341 tccgcgtctg atcaccatga tctcttcgcc aaacggtgag cagcgtctgt ttgtgccgcg
     2401 cagtggtttc ccggatttgc tggtggatac tttgctggcg acagaagacc gtcattttta
     2461 cgagcatgat ggaatcagtc tctactcaat cggacgtgcg gtgctggcaa acctgaccgc
     2521 cggacgcacg gtacagggtg cgagtacgct gacgcaacag ctggtgaaaa acctgttcct
     2581 ctccagcgag cgttcttact ggcgtaaagc gaacgaagct tacatggcgc tgatcatgga
     2641 cgcgcgttac agcaaagacc gtattcttga gctgtatatg aacgaggtgt atctcggtca
     2701 gagcggcgac aacgaaatcc gcggcttccc gctggcaagc ttgtattact ttggtcgccc
     2761 ggtagaagag ctaagcctcg accagcaggc gctgttagtc ggtatggtga aaggggcgtc
     2821 catctacaac ccgtggcgta acccaaaact ggcgctggag cgacgtaatc tggtgctgcg
     2881 tctgctgcaa cagcaacaga ttattgatca agaactctat gacatgttga gtgcacgtcc
     2941 gctgggtgtt cagccacgcg gtggggtgat ctctccacag ccagccttta tgcaactggt
     3001 gcgtcaggag ctgcaggcga aattaggcga taaggtaaaa gatctctccg gcgtgaagat
     3061 cttcactacc tttgactcgg tggcccagga cgcggcagaa aaagccgccg tggaaggcat
     3121 tccggcactg aagaaacagc gtaagttgag cgatcttgaa actgcgattg tggtcgtcga
     3181 ccgctttagt ggtgaagttc gtgcgatggt cggtggttct gagccgcagt ttgcgggcta
     3241 caaccgtgcg atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag cgacttatct
     3301 gactgcctta agccagccga aaatctatcg tctgaatacg tggattgcgg atgcgccaat
     3361 cgcgctgcgt cagccgaatg gccaggtctg gtcaccgcag aatgatgacc gtcgttatag
     3421 cgaaagcggc agagtgatgc tggtggatgc gttgacccgt tcgatgaacg tgccgacggt
     3481 aaatctgggg atggcgctgg ggctgcctgc ggttacggag acctggatca aactgggcgt
     3541 accgaaagat cagttgcatc cggttccggc gatgctgctg ggggcgttga acttaacgcc
     3601 aatcgaagtg gcgcaggcgt tccagaccat cgccagcggc ggtaaccgtg caccgctttc
     3661 tgcactgcgt tcggtaattg cggaagatgg caaggtgctg tatcagagct tcccgcaggc
     3721 ggaacgcgct gttccggcgc aggcggcgta tctgacacta tggaccatgc agcaggtggt
     3781 acaacgcggt acgggtcgtc agcttggggc gaaatacccg aacctgcatc tggcagggaa
     3841 aacagggact accaacaata acgtagatac ctggtttgca ggcattgacg gcagcacggt
     3901 gaccatcacc tgggtaggcc gtgataacaa ccagccgacc aaactgtatg gagccagcgg
     3961 ggcaatgtcg atttatcagc gttatctggc taaccagacg ccaacgccgc tgaatcttgt
     4021 tccgccagaa gatattgcag atatgggcgt ggactacgac ggcaactttg tttgcagcgg
     4081 tggcatgcgt gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc agcagagcga
     4141 gatgcagcag cagccgtcag gcaatccgtt tgatcagtct tctcagccgc agcaacagcc
     4201 gcaacagcaa cctgctcagc aagagcagaa agacagcgac ggtgtagccg gttggatcaa
     4261 ggatatgttt ggtagtaatt aacatctaag cgtgaaatac cggatggcga gttgccatcc
     4321 ggtaaaataa catcccatct aagatattaa ccctttcttt tcatctggtt gtttattaac
     4381 ccttcaggaa cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc gaatattatc
     4441 ttatctttat aataatcatt ctcgtttacg ttatcattca ctttacatca gagatatacc
     4501 aatggcgcgt tccaaaactg ctcagccaaa acactcactg cgtaaaatcg cagttgtagt
     4561 agccacagcg gtaagcggca tgtctgttta tgcacaggca gcggttgaac cgaaagaaga
     4621 cactatcacc gttaccgctg cacctgcgcc gcaagaaagc gcatgggggc ctgctgcaac
     4681 tattgcggcg cgacagtctg ctaccggcac taaaaccgat acgccgattc aaaaagtgcc
     4741 acagtctatt tctgttgtga ccgccgaaga gatggcgctg catcagccga agtcggtaaa
     4801 agaagcgctt agctacacgc cgggtgtctc tgttggtacg cgtggcgcat ccaacaccta
     4861 tgaccacctg atcattcgcg gctttgcggc agaaggccaa agccagaata actatctgaa
     4921 tggcctgaag ttgcagggca acttctataa cgatgcggtc attgacccgt atatgctgga
     4981 acgcgctgaa attatgcgtg gcccggtttc cgtgctttac ggtaaaagca gtcctggcgg
     5041 cctgttgaat atggtcagca agcgtccgac caccgaaccg ctgaaagaag ttcagtttaa
     5101 agccggtact gacagcctgt tccagactgg ttttgacttt agcgattcgt tggatgatga
     5161 cggtgtttac tcttatcgcc tgaccggtct tgcgcgttct gccaatgccc agcagaaagg
     5221 gtcagaagag cagcgttatg ctattgcacc ggcgttcacc tggcgtccgg atgataaaac
     5281 caatttcacc ttcctttctt acttccagaa cgagccggaa accggttatt acggctggtt
     5341 gccgaaagag ggaaccgttg agccgctgcc gaacggtaag cgtctgccga cagactttaa
     5401 tgaaggggcg aagaacaaca cctattctcg taatgagaag atggtcggct acagcttcga
     5461 tcacgaattt aacgacacct ttactgtgcg tcagaacctg cgctttgctg aaaacaaaac
     5521 ctcgcaaaac agcgtttatg gttacggcgt ctgctccgat ccggcgaatg cttacagcaa
     5581 acagtgtgcg gcattagcgc cagcggataa aggccattat ctggcacgta aatacgtcgt
     5641 tgatgatgag aagctgcaaa acttctccgt tgatacccag ttgcagagca agtttgccac
     5701 tggcgatatc gaccacaccc tgctgaccgg tgtcgacttt atgcgtatgc gtaatgacat
     5761 caacgcctgg tttggttacg acgactctgt gccactgctc aatctgtaca atccggtgaa
     5821 taccgatttc gacttcaatg ccaaagatcc ggcaaactcc ggcccttacc gcattctgaa
     5881 taagcagaaa caaacgggcg tttatgttca ggatcaggcg cagtgggata aagtgctggt
     5941 caccctgggc ggtcgttatg actgggcaga tcaagaatct cttaaccgcg ttgctgggac
     6001 gaccgataaa cgtgatgaca aacagtttac ctggcgtggt ggtgttaact acctgtttga
     6061 taatggtgta acaccttact tcagctatag cgaatcgttt gaaccttctt cgcaagttgg
     6121 gaaggatggt aatattttcg caccgtctaa aggtaagcag tatgaagtcg gcgtgaaata
     6181 tgtaccggaa gatcgtccga ttgtagttac tggtgccgtg tataatctca ctaaaaccaa
     6241 caacctgatg gcggaccctg agggttcctt cttctcggtt gaaggtggcg agatccgcgc
     6301 acgtggcgta gaaatcgaag cgaaagcggc gctgtcggcg agtgttaacg tagtcggttc
     6361 ttatacttac accgatgcgg aatacaccac cgatactacc tataaaggca atacgcctgc
     6421 acaggtgcca aaacacatgg cttcgctgtg ggctgactat accttctttg acggtccgct
     6481 ttcaggtctg acgctgggca ccggtggtcg ttatactggc tccagctatg gtgatccggc
     6541 taactccttt aaagtgggaa gttatacggt cgtggatgcg ttagtgcgtt atgatctggc
     6601 gcgagtcggc atggcgggct ccaacgtggc gctgcatgtt aacaacctgt tcgatcgtga
     6661 atacgtcgcc agctgcttta acacttatgg ctgcttctgg ggcgcagaac gtcaggtcgt
     6721 tgcaaccgca accttccgtt tctaatttct cttttggggc acggatttcc gtgcccattt
     6781 cacaagttgg ctgttatgca ggaatacacg aatcattccg ataccacttt tgcactgcgt
     6841 aatatctcct ttcgtgtgcc cgggcgcacg cttttgcatc cgctgtcgtt aacctttcct
     6901 gccgggaaag tgaccggtct gattggtcac aacggttctg gtaaatccac tctgctcaaa
     6961 atgcttggcc gtcatcagcc tccgtcagaa ggggagattc ttcttgatgc ccagccgctg
     7021 gaaagctgga gcagcaaagc gtttgcccgc aaagtggctt atttgccgca gcagcttcct
     7081 ccggcagaag ggatgaccgt gcgtgaactg gtggcgattg gtcgttaccc gtggcatggc
     7141 gcgctggggc gctttggtgc ggcagatcgc gaaaaggtcg aggaagctat ctcgctggtt
     7201 ggcttaaaac cgctggcgca tcggctggtc gatagtctct ctggcggcga acgtcagcgg
     7261 gcgtggatcg ccatgctggt ggcgcaggat agccgttgtc tgttgctcga cgaaccgacc
     7321 tcggcgctgg atatcgccca ccaggttgat gtgctgtcgc tggtgcaccg tttaagtcag
     7381 gagcgtggcc tgacggtcat tgccgtgttg cacgatatca atatggcggc acgctactgt
     7441 gattatctgg tcgccctgcg cggcggtgaa atgattgctc agggaacgcc tgcggaaatt
     7501 atgcgcggcg aaaccctcga aatgatttat ggcatcccga tgggtatttt gccgcatccg
     7561 gcgggtgctg cacctgtgag ttttgtttat tgatgagcgg cttacctctt atttcgcgcc
     7621 gtcgactgtt aacggcgatg gcgctttctc cgttgttatg gcagatgaat accgcccacg
     7681 cggcggctat tgatcccaat cgtattgtgg cgctggagtg gttgccggtg gaattactgc
     7741 tggcgctcgg catcgtgcct tacggcgtgg cggataccat caactatcgc ctgtgggtca
     7801 gcgaaccacc attgccggac tcagtgatcg acgtcggttt gcgcacagaa cctaaccttg
     7861 aactgctgac cgaaatgaaa ccatcgttta tggtctggtc ggcaggatat ggcccttcac
     7921 cagaaatgct ggctcgtatt gcgccggggc gtggatttaa cttcagcgac ggcaaacagc
     7981 cgctggcgat ggcgcgtaag tcactgacgg aaatggcaga tttacttaac ctgcaaagcg
     8041 cagcagaaac gcatttagcg cactatgaag actttatccg cagcatgaaa ccccgctttg
     8101 tgaagcgtgg tgaacgcccg ttattgctga cgacgcttat cgatccgcgc catatgctgg
     8161 ttttcggtcc aaacagcttg tttcaggaaa ttcttgatga gtacggcatc ccaaatgcct
     8221 ggcaagggga aaccaacttc tggggcagta ccgccgtcag catcgatcgt ctggcggcgt
     8281 ataaagatgt tgatgtgctc tgttttgatc acgacaacag caaagacatg gacgcgctaa
     8341 tggcaacccc gctgtggcag gccatgccgt ttgtccgcgc cggacgcttt cagcgcgtac
     8401 ccgcagtctg gttttatggt gcgacgctct cggcaatgca ctttgtgcgc gttctggata
     8461 acgccatcgg aggtaaagcg tgagtaaacg aattgcgctt tttccggcgt tattgctggc
     8521 gctgctggtg attgtcgcta cggcgctgac ctggatgaac ttttcgcagg cgctgccgcg
     8581 tagccagtgg gcgcaggccg cctggtcgcc ggatattaac gtcatcgagc agatgatttt
     8641 tcactacagc ttgttaccac gtctggcgat ttctctactg gtgggcgcgg gcctggggct
     8701 ggtgggcgtg ctgtttcagc aagtgctgcg taacccgctg gcggaaccga cgacgttggg
     8761 cgttgctaca ggcgcgcaac tggggattac cgtcaccacg ctctgggcga tcccaggtgc
     8821 tatggcgagc cagtttgctg cgctggcagg ggcttgcgtt gttggcttaa ttgtctttgg
     8881 cgtcgcgtgg gggaaacggc tgtcgccggt aacgctgatt ctcgcggggc tggtagtgag
     8941 cctttattgt ggggcaatca atcagttact ggttatcttc catcatgacc agttgcaaag
     9001 catgttcctg tggagcaccg gaacgctgac gcaaaccgac tggggcggcg ttgagcgttt
     9061 atggccgcag ttgctgggcg gcgtgatgct gacgttactg ctacttcgtc cgttaaccct
     9121 gatggggctt gatgatggcg tggcgcgcaa tctcgggctg gccttgtcgc tcgcgcgtct
     9181 ggcggcgctg tcgctggcga tagtcatcag tgcgctgctg gtgaacgcag tggggattat
     9241 cggctttatc gggttgttcg cgccactgct ggcaaaaatg ctgggggcgc ggcgtttgtt
     9301 gccacggttg atgctggcgt cgctgattgg tgcgttaatt ctgtggcttt ctgatcaaat
     9361 catcctctgg ctgactcgcg tgtggatgga agtttccacc ggttcggtca ctgcgttgat
     9421 cggtgcgccg ctgctactgt ggctgctgcc gcgtttacgc agcattagcg cgccggatat
     9481 gaaggttaac gatcgtgtcg cgactgaacg ccaacatgtg ctggcgtttg ccctcgcggg
//
LOCUS       DAEVZA010000166         9393 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEVZA010000166 DAEVZA010000000
VERSION     DAEVZA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.166"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1378
                     /locus_tag="LAF92_004406"
     CDS             <1..1378
                     /locus_tag="LAF92_004406"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBI9793323.1"
                     /translation="LVREDLTALDFVTIDSASTEDMDDALFAKALPDDKLQLIVAIAD
                     PTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRANEVRPVLACRMTL
                     SADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIAEQVRLLAQICQR
                     RGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEAMIAANICAARVL
                     RDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFCKLRRELDAQPTG
                     FLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINHRLLKAVIKGETA
                     TRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAAEIVDISRGGMRV
                     RLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVIDVTIAEVRMETR
                     SIIARPVA"
     gene            complement(1456..1560)
                     /locus_tag="LAF92_004407"
     CDS             complement(1456..1560)
                     /locus_tag="LAF92_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793324.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            1614..3599
                     /gene="pdeR"
                     /locus_tag="LAF92_004408"
     CDS             1614..3599
                     /gene="pdeR"
                     /locus_tag="LAF92_004408"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBI9793325.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            3746..3919
                     /locus_tag="LAF92_004409"
     CDS             3746..3919
                     /locus_tag="LAF92_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793326.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            4009..4758
                     /locus_tag="LAF92_004410"
     CDS             4009..4758
                     /locus_tag="LAF92_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI9793327.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            5027..5245
                     /gene="osmB"
                     /locus_tag="LAF92_004411"
     CDS             5027..5245
                     /gene="osmB"
                     /locus_tag="LAF92_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBI9793328.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(5371..5697)
                     /gene="yciH"
                     /locus_tag="LAF92_004412"
     CDS             complement(5371..5697)
                     /gene="yciH"
                     /locus_tag="LAF92_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBI9793329.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLADG"
     gene            complement(5697..6434)
                     /gene="pyrF"
                     /locus_tag="LAF92_004413"
     CDS             complement(5697..6434)
                     /gene="pyrF"
                     /locus_tag="LAF92_004413"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBI9793330.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(6627..7796)
                     /gene="lapB"
                     /locus_tag="LAF92_004414"
     CDS             complement(6627..7796)
                     /gene="lapB"
                     /locus_tag="LAF92_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBI9793331.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(7803..8111)
                     /gene="lapA"
                     /locus_tag="LAF92_004415"
     CDS             complement(7803..8111)
                     /gene="lapA"
                     /locus_tag="LAF92_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBI9793332.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(8260..9024)
                     /gene="pgpB"
                     /locus_tag="LAF92_004416"
     CDS             complement(8260..9024)
                     /gene="pgpB"
                     /locus_tag="LAF92_004416"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBI9793333.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            9194..>9393
                     /gene="ribA"
                     /locus_tag="LAF92_004417"
     CDS             9194..>9393
                     /gene="ribA"
                     /locus_tag="LAF92_004417"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="catalyzes the conversion of GTP to formate and
                     2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine
                     and diphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBI9793334.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCD"
BASE COUNT         2384 a         2386 c         2242 g         2381 t
ORIGIN      
        1 tctggttcgt gaagatctga ccgcgctgga ttttgtcacc atcgacagtg ccagcacaga
       61 agatatggat gacgcccttt tcgctaaggc gttgccggat gacaaacttc agctgattgt
      121 ggcgattgcc gatccaaccg cgtggattgc tgaaggcagc aagctggaca aagccgcgaa
      181 aattcgcgca ttcaccaact atctgcctgg cttcaacatc cctatgctgc ctcgcgagct
      241 ttctgacgat ctctgctcac tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat
      301 gacgctctcc gctgatggca ccattgaaga taatatcgaa ttctttgccg ccaccatcga
      361 atccaaagca aagctggtgt atgaccaggt ttctgactgg ctggaaaata ccggtgactg
      421 gcagcctgaa agtgaagcaa ttgccgaaca agtccgtttg ctagcgcaaa tttgccaacg
      481 ccgcggcgag tggcgtcata accacgcact ggtgtttaaa gatcgcccgg attaccgctt
      541 tattctcggt gaaaaaggtg aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa
      601 ccgtatcgtc gaagaagcga tgattgccgc taacatttgt gcggcccgcg tactgcgcga
      661 taagctcggt tttggcatct ataacgtgca tatgggcttt gatccggcga atgccgacgc
      721 gctggcagcg ttgctgaaaa cgcacggtct gcatgtcgat gccgaagaag tgctcacgct
      781 ggacggtttc tgcaaactgc gtcgtgaact ggacgcgcaa ccaactggtt tcctcgacag
      841 ccgcattcgt cgcttccagt catttgctga aattagcact gaacccggtc ctcactttgg
      901 cctcggtctg gaagcatacg ccacctggac ttcgccgatc cgtaaatatg gcgacatgat
      961 caaccaccgt ctgctgaaag cggttatcaa aggcgaaact gcgacgcgcc cacaggatga
     1021 aatcactgtc caaatggccg agcgtcgccg tctcaaccgg atggcagaac gtgatgttgg
     1081 tgactggtta tacgcacgct tcctgaaaga caaagccggg accgacaccc gtttcgcagc
     1141 ggaaattgtc gatatcagcc gtggcggcat gcgtgttcgt ttggttgata acggcgctat
     1201 cgcctttatt ccggcacctt tcttacacgc tgtgcgcgat gaactggttt gcagccagga
     1261 aaacggcact gtacaaatta aaggtgaaac ggtttacaaa gtgactgacg ttattgacgt
     1321 cactattgcc gaagtccgca tggaaacccg cagcattatt gcgcgcccgg tcgcgtaatc
     1381 tcctttcacg gcccattcct catggatggg ccgtttattt ccccgctcta ccttcgttat
     1441 atccaggcag attttttaac tgactttcgt ttgaaaactg gcgtttttcc aaaactggat
     1501 tacaaataaa tatataaaaa aaacaatgcg tttagccgcc gttaatttca tgtaagtcat
     1561 gttgttcctt ttatttaacc gcggactccg cagttaaccg gaggatatgc atcatgaaaa
     1621 ccgttaggga gtccacaacg ttgtacaact ttctcggatc gcacaatcca tactggcggt
     1681 tgacggaaag cagcgatgtt ttgcgctttt ctaccaccga aaccacagaa cctgatcgta
     1741 ctttgcagtt atctgccgaa caggctgctc gcatcaggga aatgacggtc atcacctcca
     1801 gcctgatgat gagtctgacc gtcgatgaga gcgatctttc tgtgcatctg gtaggacgaa
     1861 aaatcaataa acgggaatgg gctggcaacg cgtctgcatg gcatgacaca ccggcggttg
     1921 ctcgtgattt atcacacggg ctttcctttg ctgagcaggt agtttctgaa gcacattccg
     1981 caatagtcat tctcgacagc cgggggaata ttcaacgctt caatcggtta tgtgaagatt
     2041 acacagggtt gaaagaacac gacgtcattg ggcaaagcgt gtttaaactg tttatgagcc
     2101 gtcgtgaagc tgcggcatcc aggcgcaata accgtgtatt ttttcgaagc ggcaatgcat
     2161 atgaagtcga actgtggata ccaacatgta aaggccagcg gctgtttctg tttcgcaata
     2221 aatttgtcca cagcggcagt ggcaaaaacg agattttttt aatctgttcc ggcaccgaca
     2281 ttaccgaaga gcgccgcgct caggagcgac tgcgtattct ggcaaatacc gacagtatca
     2341 ccggactgcc gaatcgtaac gcaatgcagg atttaatcga tcacgctatt aatcatgcag
     2401 ataacaataa agttggggtt gtgtatcttg atttggataa tttcaaaaag gtcaacgacg
     2461 cctatgggca tttgtttggt gaccagttat tacgcgacgt gtcattggct attttaagct
     2521 gtctcgaaca tgaccaggtg ttggcgcgtc caggtgggga tgagtttctg gtactggcat
     2581 ccaacacctc acaaagcgcg ctggaagcaa tggcatcacg aattttgacc cgcttacggc
     2641 tcccctttcg cattggttta attgaagttt ataccagctg ttcagtaggt attgcactct
     2701 ctcccgaaca tggttcagac agcacggcta ttattcgtca cgccgacaca gcaatgtaca
     2761 cagcgaagga aggcggacga ggacaatttt gcgtttttac tccagaaatg aatcagcggg
     2821 tatttgaata tctctggctg gataccaact tgcgtaaagc actggaaaac gatcagttgg
     2881 ttattcacta tcaaccgaaa atcacctggc gtggcgaagt gcgcagtctg gaagcactag
     2941 tacgttggca gtcacctgaa cgtgggttga ttccaccgtt ggacttcatt tcctacgccg
     3001 aagagtcagg gctaattgtg cctttaggcc gttgggtgat tctcgatgtc gtacgccagg
     3061 tggcaaagtg gcgggataaa ggcataaacc tgcgagtggc ggtaaatatt tctgcacgtc
     3121 agctcgccga tcaaaccatt ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg
     3181 aatactgccc tatagatgtt gaactgacag agagttgtct gattgagaat gatgaactgg
     3241 cactgtctgt tattcaacaa tttagccaac taggtgcgca agtgcatctg gatgattttg
     3301 gtaccggcta ctcttcactt tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg
     3361 accaggtttt tgttcgagat attcacaaac aacctgtctc gcagtcactg gtccgggcga
     3421 tcgtcgctgt ggcccaggca ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa
     3481 aggaagatgc ttttttaacc aagaacggga tcaatgagcg gcaaggattt ttgtttgcca
     3541 aaccgatgcc cgccgtcgcc ttcgaacgct ggtataaacg ctatctgaag cgcgcataag
     3601 tagatgaata tatatactta gcgctatatc ccgtcattat atatcgtatt cctggatgat
     3661 aattagccgt tgctgttaaa cacttctctt ttagcggcaa tttttgcatt attcattttc
     3721 cacttttgct ataaggatgt ccttgatgtc tgaatttgat gcccagcgcg ttgccgaacg
     3781 tattgatatt gtgttagata tccttgtggc aggcgattac cactctgcga tccataatct
     3841 cgagatactt aaagcagaac tcttgcgcca ggttgctgaa tctacgccag acatcccgaa
     3901 ggcaccgtgg gagatttaac aatcgtgcct tcaggttttc ctatttgagg atgcccggcg
     3961 cgtcagtatt tttttattta gtattataac gttataagaa ttacagcgat gaattcccga
     4021 caacaaacta ttctacagat ggtcattgac cagggtcagg ttagcgtaac cgatctggca
     4081 aaagccactg gagtttctga agttaccatt cgccaggatc tcaacaccct cgaaaaactg
     4141 agttacctcc gccgtgcaca tggctttgca gtttcgcttg atagtgatga cgtcgaaacc
     4201 cgtatgatga gcaactatac gctgaagcgt gaactcgccg agtttgccgc gtcactggtt
     4261 caaccgggcg aaaccatctt tatcgaaaat ggtagcagca atgccctgct cgcccggact
     4321 ctgggcgaac agaagaaaaa tgtcactatc atcacagtca gcagctacat tgcgcatttg
     4381 ctgaaagacg cgccttgtga agtcattttg ctcggtggcg tgtaccagaa aaaaagcgaa
     4441 agtatggttg gcccgttgac acgccagtgc atccagcagg tgcatttcag caaagcattt
     4501 attggtattg atggctggca acctgaaact ggattcaccg gtcgcgatat gatgcgtacc
     4561 gatgtggtca atgccgtgct ggaaaaagag tgcgaagcga tagtcctgac tgacagctcg
     4621 aaatttggtg ctgtacattc atactccatc ggtcccgttg agcgattcaa tcgcgtgatt
     4681 accgattcga aaatacgcgc cagcgatctg atgcatcttg agcacagtaa actcaccgtt
     4741 cacgtcgttg acatttaacc atttcccttc tctacggatg atttgcagtt tggcaaatca
     4801 tccgctctaa gatgattcct ggttgataat taagactatt tacctgttat taacactctc
     4861 aagatataaa attattatca gcgatataac aggaagtcat tatcacctgc gtgatataac
     4921 cctgcgcgcg agcagatttc acggaataat ttcaccaggc ttattcttag ctattatagt
     4981 tatagagagc ttacttccgt gaatcataaa ttcaggagag agtattatgt ttgtaacgag
     5041 caaaaaaatg accgcggctg ttctggcaat tactttggca atgtctctga gtgcctgttc
     5101 taactggtct aaacgggacc gcaacaccgc aatcggcgcg ggtgcagggg cattaggcgg
     5161 tgcagtactg accgatggca gtacgttggg tacattaggt ggtgcagctg tcggtggtgt
     5221 tattggtcac caggtcggta aataatcgta aataaatgtc gattatcgac tcgcaattat
     5281 aatacgcttg attctgttca gtacgaaata actgaataat acgcacagta aaatattcga
     5341 aagccacgga tatatccgtg gctttttatg ttaaccgtct gcgagtttta ccttcatccc
     5401 tttcgcttca agtagtgatt ttaataaatc acgcttatcg ccctggattt caataactcc
     5461 atctttaact gctccgccgc agccgcattt tttcttcagt tccgctgcaa gttttgtcag
     5521 ttcggcatca tcgagatcga caccggtaat caggcaaacg cccttacctt tacgtccact
     5581 ggtctgacgc tgaatacgca ccacaccgtc gcctttaggg cgtacagggg ccgctttggg
     5641 ctcatcaata cgtccggttt ccgttgagta gaccagacgg ctgttggaat cactcatcat
     5701 gcactccgct gtaaagaggc gttgatcgct ttcagcgtct gcgctggatc taccgattgc
     5761 gttaccgggc gaccaatcac catataatca acaccagccg acaacgcctg ttctggcgtc
     5821 ataatgcggc gctggtcacc agcttcactc ccctgcggac gaatgcccgg cgtaaccagt
     5881 ttgaactcct gaccgaatac ctgtttaaag cgcacagctt cctgagcaga acacaccaca
     5941 ccatcaaggc cacatttttg cgtcagtgcc gccagacgtt ctgcataatc tgcaggtgac
     6001 agtgtcatgc caagatcgac caggtcgctg gcttccatgc tggtcaacac tgtcacagca
     6061 atcaaaagcg gtgcatcttt gccaaacgga accagtgcct cacgcgctgc ggtcatcata
     6121 cgcgccccac cagaggcatg aacattcacc atccacacgc ctaagtcagc tgcagcagcg
     6181 acagcgtgcg cagcagtgtt aggaatatcg tggaatttca ggtcaagaaa gatatcaaaa
     6241 ccacgctgtt gaagttcgcg cacaaactgt ggcccaaaca atgtaaacat ctctttgccg
     6301 accttcagac gacaatcgcg tgggtcgatc ttgtcgacaa aggacagcgc gtcatcacga
     6361 ttatgataat caagggcaac aaccacagga gaattcgtaa cagcgcggga agaagatgaa
     6421 gcagttaacg tcatgaccag accttcttga tgatgggcac cggaagtggc gcgaaacagg
     6481 taaacggcga gcattctacc cgtggaaata aaaaattaac agttgcgatt tttcccctgg
     6541 caggcttttt ctgcgccgtc gttgccggag cacaatggaa catcaataac tagtatgttg
     6601 taactaaagt cggatttttt taaaaattac aggccatcaa gaccgcgaat cggtttaatg
     6661 gttgaccagg cccgacaaga cggacaatgc cagtagaggg tgtatgcggt aaaaccacat
     6721 ttctggcagc gataacgagg cttactacgt accttttcgc caaccatgtc acgcagcacc
     6781 atcaggctct ctttggcacg cccttcttcc gcttcattta agtggtaatc cattaactta
     6841 tggaacacac gcatggtcgg atgacgctga agctggcgcg taatatagac ctgtgcggcc
     6901 tcactaccgt cgcgcgcttc gatgatatca gcaagcatca attcagcatc ggcaccggtg
     6961 ttctcttcca ccgcgcgttg caggaattct gcccattcgg cagttttacc caactgctga
     7021 tagcaggttt gcaacatttc cagcgtttcg ctgaccagtt ctctgtcctg ggatatcacg
     7081 cgttgcaaac tttcgacggc tttggcgtat tctcctttcg ccataaacac gcgtcccatc
     7141 atgatggata cgcgggcgct gtttttatct gccgccgccc ctttttttag caaggtcatg
     7201 gcacgatcga gatcgtcgct ggccatatgc tgcagggcta actcacagta gaaatgggca
     7261 atttcgacgc gctgtttatc tttacccagc ttcaccaggc gttcggcaac atcaattgct
     7321 ttctgccact cactggtagc ctggtagatt tgtagcaact gttgcagagc gccaatgcgg
     7381 aagtcagttt catcggtcag ctgattgaac atgtcttccg cgcggtcgta taacccggcg
     7441 gccatgtaat cacgccctag ttgttgaatc gccaacagac gctgttcata ggtcagcgag
     7501 gcgctttcca ttagggtctg atggatgcga atagcgcgat caacttcgcc acgcgaacgg
     7561 aacaggtttc cgagcgtaag gtgggcttca acggtgcctg tatcctcttt aagcatatcg
     7621 agaaacaggt ctaccgcttt atcctgttga ttactaagca ggaagttaac ccccgctacg
     7681 taatcacgcg acaagcggtt ggcttcatct tgcttgtttt gttgcgcact tctgcggccc
     7741 atataccagc cataggcagc ggctacaggc aaaagcagaa acaacaactc cagcatagaa
     7801 agttattcct tcgccgctga cgagtgcggc actacagcca cgtcagtcgc gggtgaaagc
     7861 tggttttcca gtcgctttat tttacgttca gcgcgcgcca gggaaacacg aactcgcagc
     7921 cagaacaggc cacaaatcaa ccaaccgata gcaaaccccg cagcaaacaa taccgccagc
     7981 aatgtggaaa tacggtactc cccttgcgct aacagataat taaacgtcac ctgttgatcg
     8041 ttctgcgcac ccaacgtgac cgaaatcacg aagatcgcta acaccagtaa gaaaatgagt
     8101 aaatatttca cattacttcc cgttatgtgg ttagagcgaa taaattatgg tcaacttacc
     8161 gcaatagccc tttaaagtac cattttcgcg ccgtctgcga aatggcatcc acgcgcattg
     8221 ttgatgattt aagggctaaa ttcggaaaat caaccagcgt taactttctt gttctcgttg
     8281 cgctatttcg cgattttctt ccgcaggtgg tgttaatggc ccacaaattc gttgcgcaag
     8341 ccacgttgcc accgccacca gcgcccacga aatcaacgta gctactacca gatcgcgtgg
     8401 ccaatgcatc ccgagcagca ggcggcttcc catgactccc gttgcccaga ccagcaagat
     8461 agcaatggtt aacgttcgcc gacgcggcca cagcaaacca acggccagca gtgcccaact
     8521 ggcagcaaac atcgtgtgac cggaaggaaa ggcaaacccc gtctctttct gccagtgtga
     8581 acgcaaatat tgtgggatat ttttctcttc agccaactgt tctttcacta gatttccgcg
     8641 ttctgctcgc tttaaagtgt agaactcatc aaccggaata tgatgtgttt tttccagcca
     8701 gataacaaaa ggtcgtggtt cctggacttt gtctttgatc caggatttaa cgccttgtcc
     8761 cacaaggatt gcggccgcca gaatggcaaa taatacaaag gcagccttaa tgcgaaaacg
     8821 cagacaccag agaaaccagc cgaataaaat caaatgtgta atgacgcccc agggctgggt
     8881 gacagtttca gtaacccaaa aagccgcttt tagtagccaa ctttgttctc caggttgcca
     8941 acgccagcca gaaatccata cggctactgg catgacaagc aatagtgcag ctcccactgc
     9001 ggtacgtctg gcaatcgaac gcatggcctc tcctttttga taagtcccac aatcataact
     9061 gaaaacgcca gttccaggaa aaattgacag atttgtgcca ttccgtgaac gatcgacgcg
     9121 tcgtgattag gtgaacccct tctcgttatg gcaaaataag ccaatacaga accagcatta
     9181 tctggagaat ttcatgcagc ttaaacgtgt ggcagaagcc aaactgccaa ccccatgggg
     9241 cgatttcctg atggtgggat ttgaagaact ggcaaccgga cacgatcatg tcgcgctagt
     9301 ctatggcgat atttccgggc ataccccggt acttgcgcgc gtccattccg aatgtctgac
     9361 cggtgacgcc ctgttcagct tgcgctgcga ttg
//
LOCUS       DAEVZA010000167         9278 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEVZA010000167 DAEVZA010000000
VERSION     DAEVZA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.167"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..556
                     /gene="nfsB"
                     /locus_tag="LAF92_004418"
     CDS             <1..556
                     /gene="nfsB"
                     /locus_tag="LAF92_004418"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBI9793335.1"
                     /translation="LQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGNYVFNERKILD
                     ASHVVVFCAKTAMDDAWLKLVVDQEDADGRFATPEAKAANDKGRKFFADMHRKDLHDD
                     AEWMAKQVYLNVGNFLLGVAALGLDAVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGH
                     HSVEDFNATLPKSRLPQNITLTEV"
     gene            664..1911
                     /gene="ybdG"
                     /locus_tag="LAF92_004419"
     CDS             664..1911
                     /gene="ybdG"
                     /locus_tag="LAF92_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBI9793336.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(1979..3355)
                     /gene="pheP"
                     /locus_tag="LAF92_004420"
     CDS             complement(1979..3355)
                     /gene="pheP"
                     /locus_tag="LAF92_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBI9793337.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(3457..6600)
                     /gene="cusA"
                     /locus_tag="LAF92_004421"
     CDS             complement(3457..6600)
                     /gene="cusA"
                     /locus_tag="LAF92_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBI9793338.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(6612..7835)
                     /gene="cusB"
                     /locus_tag="LAF92_004422"
     CDS             complement(6612..7835)
                     /gene="cusB"
                     /locus_tag="LAF92_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBI9793339.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDQAEPHAEKTPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(7851..8183)
                     /gene="cusF"
                     /locus_tag="LAF92_004423"
     CDS             complement(7851..8183)
                     /gene="cusF"
                     /locus_tag="LAF92_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBI9793340.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(8207..>9278)
                     /gene="cusC"
                     /locus_tag="LAF92_004424"
     CDS             complement(8207..>9278)
                     /gene="cusC"
                     /locus_tag="LAF92_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBI9793341.1"
                     /translation="EGSGSWSGNLNGDSATTREFSTGLNASFDLDFFGRLKNMSEAER
                     QNYLVTEEAQRAVHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLT
                     GSSNVLALEQARGVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQ
                     SVKLPAGLSSQILLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLS
                     SLFNASSGMWNFIPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADA
                     LALRQSLNDQISAQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLD
                     LNYARQVNEISLYTALGGGWQQ"
BASE COUNT         2216 a         2596 c         2288 g         2178 t
ORIGIN      
        1 cctgcaatac agcccatcca gcaccaactc ccagccgtgg cattttattg ttgccagcac
       61 ggaagaaggt aaagcgcgtg ttgccaaatc cgctgccggt aattatgtgt tcaacgaacg
      121 taaaatactt gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc
      181 ctggctgaag ctggttgttg accaggaaga tgctgatggc cgctttgcca cgccggaagc
      241 gaaagccgcg aacgataaag gtcgcaagtt cttcgctgat atgcaccgta aagatctgca
      301 tgatgatgca gagtggatgg caaaacaggt ttatctcaac gtcggtaact tcctgctcgg
      361 cgtggcggcg ctgggtctgg acgcggtacc catcgaaggt tttgacgccg ccatcctcga
      421 tgcagaattt ggtctgaaag agaaaggcta caccagtctg gtggttgttc cggtaggtca
      481 tcacagcgtt gaagatttta acgctacgct gccgaaatct cgtctgccgc aaaacataac
      541 cttaaccgaa gtgtaattct ctcttgccgg gcatctgccc ggctatttcc cctcagattc
      601 tcctgatttg cataaccctg tttcagccgt catcataggc tgctgttgta taaaggagac
      661 gttatgcagg atttaatatc ccaggttgaa gatttagcgg gtattgagat cgatcacacc
      721 acctcgatgg tgatgatttt cggtattatt tttctgaccg ccgtcgtggt gcatattatt
      781 ttgcattggg tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc acggctttgg
      841 ttgcaaatca ttacccagaa taaactcttc caccgtttag cttttaccct gcaggggatt
      901 atcgtcaata ttcaggcggt attctggctg caaaaaggca ccgaagcggc agatattctg
      961 actacctgcg cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg
     1021 gatgttattt tgaatctggc gcagaaattc ccggcggcat ctcagttacc gctgaaaggg
     1081 atatttcagg ggattaaact gatcggcgca atcctggtcg gcattttgat gatctcgctg
     1141 ctgattggtc agtcgccagc gattctgatc agcggtcttg gtgcaatggc tgccgtgctg
     1201 atgttggttt ttaaagatcc gattcttggt ctggtggcag gtattcagct ttccgcgaac
     1261 gatatgctga aactgggcga ctggctggag atgccgaaat acggcgcgga tggcgcggtg
     1321 atcgatattg ggttaaccac cgtcaaagtg cgtaactggg acaataccat taccactatt
     1381 cccacctggt ctctggtttc tgactccttt aaaaactgga gcgggatgtc agcatctggc
     1441 gggcgacgta ttaagcgcag tatcagtatt gatgtcacca gtattcgttt tcttgatgaa
     1501 gacgaaatgc aacgtctgaa taaagcgcac ttgttaaaac cttatttaac cagccgccat
     1561 caggaaatta atgagtggaa tcgccagcaa ggttctacgg agtcgatatt aaacctgcgc
     1621 cgaatgacca atattggaac ctttcgtgcc tatctgaacg aatatctgcg taaccatccg
     1681 cggattcgta aagatatgac cttaatggta cgccaactgg ctccaggtga taacggttta
     1741 ccgctcgaga tctacgcgtt tactaacacc gtggtgtggc tggaatatga aagcattcag
     1801 gctgatatat tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg acttcatcag
     1861 tcgccaaccg gcaatgatat tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa
     1921 ccgctctcat cgaatggatg agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt
     1981 atttccgacg cagcgtttta aatgccacaa acaggaatac aatccacacc ggcagcagga
     2041 tcgctgacaa gcgcatatca tccatcgtgc acatcagcag caaaatcatg ccgaggaagg
     2101 caatgcaaag atagttgccg aacggataga gcagcgcctt aaactgtgtt tcacgcccct
     2161 gacgtcgcat cgccgcgcga aaacgcagat gcgccaggca gatcataatc cagttcaaca
     2221 gcagcgttgc taccaccagc gccatcagca gaccaaacgc tttttgcggc agcagatagt
     2281 tgattaacac caccagcgaa gtgatcgctc cggaaagcat cagcgagtta atcggcacac
     2341 cgcgacggct gacgcgagtc aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca
     2401 gcatgcggct gttagagtaa accccgctgt tatacactga cagcgatgct accagaatga
     2461 cgaagttcag cgcagaagct accacgttgc tgtcgagatt atggaaaatc atcacaaacg
     2521 ggctactgtt ggatttcact tccacccacg gatagagcgc cagtaaaacc accagtgaac
     2581 cgatataaaa cagcaggatg cgatacacca cctgattcac tgctttcggg atgctttttt
     2641 ccggatcgcg cgcttcagcg gcagtaatcc caatcagctc cagaccgccg aaggagaaca
     2701 taattaccgc cagcgacaaa atcagcccat tccagccggt ggcgaagaaa ccaccgtagc
     2761 gccagaggtt gtcgatactg gctttctcgc cgccgtgacc agaaaacagc agccacaggc
     2821 caaagccgat cataccgatg atcgccagca ctttaatcag cgcaaaccag aactcggttt
     2881 caccatataa gcgcacgttc accaggttaa cggcgttgat gataataaag aaggcggcag
     2941 cccaaatcca cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca
     3001 gctctgccat tcccaccagc acgaacatta cccagtagtt ccagccagag aggaagcccg
     3061 caaacggtcc ccagtattta taggcaaagt gggcaaatga accggatacc ggctcttcga
     3121 ccaccatctc gccaagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc
     3181 ccagcaatac agccggaccc gccatctgaa tcgccgggcc aatgccaaga aacagaccag
     3241 taccaattgc gccacccagc gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa
     3301 gcgtcggctc ttgattcgac gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt
     3361 tcccctgtgt gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa
     3421 gacacaatcc acacggttaa acggggtatc ctgcttttat ttccgtaccc gatgtcggtg
     3481 cagccacatc agcttatacg ccgccgggat aataaacagc gacagcaaag gtgcggtgat
     3541 catgccgcca atcatcggtg cggcaatccg gctcatcacc tctgaaccag cccccgttcc
     3601 ccacagaatc ggcagcagac cggcgataat caccgccacc gtcatcgctt tcgggcgcac
     3661 gcgcaggacc gcgccgtgat ataacgcctc atccagcttc tgctcactga atgtttgcgg
     3721 attattcaac gacggctcgg cctctatggc gtgacgtaaa tacatcagca tcaccacgcc
     3781 aaattcggcg gcgaccccgg cgagggcgat aaagccagtg cccgtcgcca cggaaagatg
     3841 aaagcccatc caccacagca accagatgcc gcccaccagc gcaaacggta cgctgctgat
     3901 aatcagcaac gcttcgccca cccgacggaa cgccagatac aacagcacga agataatcat
     3961 caacgtcatc ggcaccatga gtttaagctt atggttggcg cgctccagta gctcgaactg
     4021 cccggagaat gccacgctgg tgcccggttt taactgcact ttttcagcta tcgctttttg
     4081 caaatcgtga accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt
     4141 cgggcgcgca ttctcggttt tcagcattga cggtccggta gagactttaa tgtcggccac
     4201 gtctgccagg gtgatttgct gcttcatcgg cgtcagaatc ggcagctggc gcagtgcctg
     4261 cggactatcg cgccagcttt gcggataacg cagattaatt ggataacggg caattccttc
     4321 caccgtttcg ccaaccatcg ccccgcccac agcagaagtc acaaacaact gcacatccgc
     4381 caccgtcata ccgtaacgcg cggctttttc acggttaatc tcaacgttga tatagcgccc
     4441 accttccagc cgctcggcaa gagcagaagc tacgcctggc accgttcgcg ccacttcttc
     4501 aatttgctca gccatcgcgt cgatatccgc cagcacagtg ccggaaactt taatgccgat
     4561 ggggctttta atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag
     4621 attcgccagc cccggcagac gcacggtgtt atccagttcc tcaatgattt tgtccatcgt
     4681 catgcctggc cgccactgct cctgcggctt aagctggatg gtcgtttcta ccatctccag
     4741 cggagcagaa tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc
     4801 aggtacgctc ataattagct tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga
     4861 aatccccggc agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa
     4921 ttccccgcca actttattga gcggccagag aaccgtcagc accgaaagcg ccgccaccag
     4981 cagcgtggtt ttcggccagt gcagtacttt cagcaacagc ggatgataaa cacgaatcaa
     5041 aaagcgattg agcgggttac tgctttccgg cggaattttg ccacggatcc agtagcccat
     5101 caggatcggg atcactacga tcgccagcag cgccgcaccc gccatcgcat acgttttggt
     5161 gaacgccaac gggccaaaca gacgcccttc ctgcccttcc agggtgaaga tcgggataaa
     5221 cgacaacgtg ataatcagca gactgataaa tagcgccgga cccacttcaa cagatgcatc
     5281 ggtgatcacc tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca
     5341 ctcttccagc cgtttatgtg cattttcgat catgacgata gcggcatcga ccatcgcccc
     5401 gacggcaatc gcaatgccgc ccaacgacat aatattggca ttcagtccct ggaagtgcat
     5461 gacaataaaa gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg
     5521 cacatgccag agaaacagcg cacagactac cgccaccaca ataaactctt ccagcaactt
     5581 gccgctgagg ttgtcgatgg cgcggtcaat gagctggctg cgatcgtatg ttgtaactat
     5641 ctccacgcct tccggcagac tacttttcag cgtttccagt ttgtccttca cggcggcgat
     5701 cacttctcgg gcgtttttgc cggatcgcag gatcaccacc ccgcccgcca cttcaccttc
     5761 gccattcagt tcggcaatgc cccggcgcat ctccgggcca atctggacct tcgcaacatc
     5821 acgcagataa acgggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc
     5881 gagcgtttgc agatagccgc tggcgcgcac catatattcc gcttccgcca gttcgatcga
     5941 cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg ctagactgat
     6001 gccatattgc gccaggcgct ggggatcgat aaccacctga tactctttca ccacgccgcc
     6061 caccgacgcc acttccgcaa cgtcagggat ggtttttagc tcatatttga gaaaccagtc
     6121 ctgcaatgag cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca gtgcatattc
     6181 atagatccag ccaacacccg tggcatctgg tcccagctcg gcgctgactc ccgcaggcag
     6241 cttaccctgt acctggttga ggtactccag cacccgcgaa cgcgcccagt acggatcggt
     6301 accatcttcg aaaatgacat acacataaga gtcgccaaac tgtgagaaac cgcgcacagt
     6361 cttcgcgcca ggcaccgaca acatggtggt ggttagcgga taagtcacct gattttcaac
     6421 gatttgcggt gcctgaccgg gatagctggt tttaataatc acctgcacat cggagagatc
     6481 cggcagcgca tccactggcg tgttaatgat ggtccaggtg ccccagatgc tcagaaacaa
     6541 cgcgcccatc agcaccaaaa aacggttcgc caccgagcga cgaataatcc attcaatcat
     6601 tggttattcc ctcaatgcgc attggtagca ctttcagagc gcatccgctc cagtgcgcca
     6661 gaaatattgg cttcggaatc aatcaggaac aggccgctgg aaaccacctt ttcaccttcc
     6721 gccagaccag agtgtaacgc ggtgacgcct tgcgatgcct ggaaaacggc aacgcgtttc
     6781 ggtacaaagc gtccgtcggc atcaacggta atcacccgct gttcgttgcc ggtatcaatc
     6841 agcgcctgtg acggaatgag cagcatcggt tcgctggcgg tattgagttg caaccaggcg
     6901 ttcattcccg gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg
     6961 cgggtcgcgg catccacgcc aggtagcagc gtccatttgc ggatggtgag tgttttatcc
     7021 ggtcgcgccg gaacggtgag ggtaaactgc gaggcatctt tcaccagcca ggcgatggac
     7081 tccgggatcg cagcagtgac ccacaccggg tccataccct gaattttcgc taccacgtta
     7141 tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggt
     7201 gctttgagcg taaagcgagt ctggattttt tgcgtggcga tcaggcggcg aatatccgcc
     7261 tccggcattc ccgccagtct cagccgctca agaatgcctt cagtctgggt cgccgtaccg
     7321 ccggtttcgc gcagcagtaa atactcactc tgcgcttcca cccagtcagg aatggtcagg
     7381 tcgagaagcg gcgcgccctt ttgcacttta tcgcccacgg taagcggata caccttgtcg
     7441 ataaacccgg cggcgcgagc ctgcacaatg gcatactgat actcgttgta actgacattc
     7501 gccgggaaac tctgggcaaa agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc
     7561 agattctgcg tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcggca
     7621 tatttcggca ccagatccat atccataaac ggcgatttac ctggtttatc gaaccgcgta
     7681 ttaggataca tcgggtcgta ccagaataag attttacgtt ctgcggtcgg cgttttttct
     7741 gcgtgcggtt ccgcctggtc aaaccaggta aaacctgccg cagaaataat accgcccgcg
     7801 atcatgctgc cgataataag cgcgattttt ttcatctcat taaacctggg ttactggctg
     7861 actttaatat cctgtaataa agaaaggttg ccctgctgga cgaaattaaa cgccactttg
     7921 tcgccggttt taatttcact cattttcgtc tgcggggtga tggtaaagcg catggtcatc
     7981 tccggccagt tgacggcagc aatcggatcg tgatggatgg tgattttttt gctttccaga
     8041 tcaacacctt ttaccacacc ggtggcacta atgacctgtg gttgtgcttc gctcatggtt
     8101 tcatgatgat gttcgttagc ctgggcatta aagccaataa cagtaaacag actgaacatt
     8161 gcgacttgca gtgctttttt catttattct ctcctggagt taaaaattat tgctgccaac
     8221 cgccaccaag tgcggtatat aaagaaattt cgttaacctg acgggcataa ttcaggtcga
     8281 gtaaagtttg tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta
     8341 ccgcgccgtg ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gacgccagat
     8401 aacgctgctg ggcgctgatt tgatcgttca ggctttgacg taatgccagc gcatctgcca
     8461 cttctttaaa ggcgttctgg attttctgtt cataattcac caccgactgc tgctggcgaa
     8521 tttcggcgat atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt
     8581 taggaataaa attccacatc ccgctgctgg cgttaaataa tgacgacaga tcgctgctgg
     8641 cggttgatat tccgctggtc aggctgatag acgggaaaaa tgccgcacgt gcagcaccaa
     8701 tattggcatt agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata
     8761 agatttgcga cgacaagccc gccggtaatt taacgctttg caggctgtcg ctgtttactg
     8821 tctgcgcttg cggcagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct
     8881 gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt actttctatt accccgcgag
     8941 cctgttccag cgccagaaca ttgctgctac cggtcaacag ttgtttttcg acaaacgcat
     9001 atgactgctg ataattgcgc agcgtttctt cggctatttg caattgcgca tacgccagtt
     9061 gctgattgaa atagctttgc gcgacattag aaaccagcag aatatgcacc gcgcgctgag
     9121 cttcctcagt gactaaataa ttttgtcgct cggcttcgct catgttcttt aagcgaccga
     9181 aaaaatcgag atcaaagctg gcgttaaggc cagtcgagaa ctcccgcgtc gtggctgaat
     9241 cgccgttaag attgccgctc cagctgccgc tgccctcg
//
LOCUS       DAEVZA010000168         9233 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEVZA010000168 DAEVZA010000000
VERSION     DAEVZA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.168"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(79..1317)
                     /locus_tag="LAF92_004425"
     CDS             complement(79..1317)
                     /locus_tag="LAF92_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBI9793342.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            1653..2855
                     /gene="nupC"
                     /locus_tag="LAF92_004426"
     CDS             1653..2855
                     /gene="nupC"
                     /locus_tag="LAF92_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBI9793343.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(2905..5094)
                     /locus_tag="LAF92_004427"
     CDS             complement(2905..5094)
                     /locus_tag="LAF92_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HBI9793344.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5303..5378)
                     /locus_tag="LAF92_004428"
     tRNA            complement(5303..5378)
                     /locus_tag="LAF92_004428"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5343..5345),aa:Ala,seq:ggc)
     gene            complement(5418..5493)
                     /locus_tag="LAF92_004429"
     tRNA            complement(5418..5493)
                     /locus_tag="LAF92_004429"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5458..5460),aa:Ala,seq:ggc)
     gene            5714..6073
                     /locus_tag="LAF92_004430"
     CDS             5714..6073
                     /locus_tag="LAF92_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBI9793345.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            6075..6467
                     /locus_tag="LAF92_004431"
     CDS             6075..6467
                     /locus_tag="LAF92_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBI9793346.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(6519..7934)
                     /gene="gltX"
                     /locus_tag="LAF92_004432"
     CDS             complement(6519..7934)
                     /gene="gltX"
                     /locus_tag="LAF92_004432"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBI9793347.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            8193..8268
                     /locus_tag="LAF92_004433"
     tRNA            8193..8268
                     /locus_tag="LAF92_004433"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8226..8228,aa:Val,seq:tac)
     gene            8313..8388
                     /locus_tag="LAF92_004434"
     tRNA            8313..8388
                     /locus_tag="LAF92_004434"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8346..8348,aa:Val,seq:tac)
     gene            8435..8510
                     /locus_tag="LAF92_004435"
     tRNA            8435..8510
                     /locus_tag="LAF92_004435"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8468..8470,aa:Val,seq:tac)
     gene            8515..8590
                     /locus_tag="LAF92_004436"
     tRNA            8515..8590
                     /locus_tag="LAF92_004436"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8548..8550,aa:Lys,seq:ttt)
     gene            8711..9052
                     /locus_tag="LAF92_004437"
     CDS             8711..9052
                     /locus_tag="LAF92_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBI9793348.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQCLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(9043..>9233)
                     /locus_tag="LAF92_004438"
     CDS             complement(9043..>9233)
                     /locus_tag="LAF92_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9793349.1"
                     /translation="LPLPEGSPLVVKRITPVVERQLMLVRRKNRSLSTAAEALWDVVR
                     DQGNALMAGREGDPLYQI"
BASE COUNT         2463 a         2402 c         2163 g         2205 t
ORIGIN      
        1 accggctctt taacgcgtag ggaggataag gcatttacgc cgcatccgcc aatggtgcca
       61 gatgcgacgc tcattcaact acaatcccag cgccgtcccc accaacaacc agatattcag
      121 cgccacgacc agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag
      181 atcgcccatc aacttgctgt cactggtgaa aatcagcagt ggaactagcg ccagggcgat
      241 accaaaactt aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag
      301 aatgacaata aatgacggca gcatggtgac tgtacgacgc acccacagcg ggatatggaa
      361 gcgaatgaag ccctgcatca ccacctgccc cgccagtgtc cccaccaccg ttgaggacag
      421 tccggcagca accaggctta acccaaagac cgttgccgca gcatggctta acagcggttg
      481 cagcgtcaga taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc
      541 cgccgcagct gtagccatca tcgccagatt gacaaaaccg gcaatagtca tggcgatagc
      601 cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt
      661 gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc
      721 caggaagacc gcctcagaag taggtaaact cgggatcacc attcctttac ccagctgcgc
      781 caggttaggc tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa
      841 cccgccaatc actttctcca gcggtttttg cccgcgacgt tgcagcatta aaatcaggaa
      901 agtcgcgatc cccgtcagca ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa
      961 accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg
     1021 aacccaatag aaccacacta cgggacgcgg atagtgatcg cgaatctgct ccgccagatt
     1081 tttaccggtg gcaatcccca gtttggcaga gaggatctga atcagcatcg ccatcaggtt
     1141 ggcccaaacg acaacccaca gtagctgata gccgaagctg gcacccgcct gaatattggt
     1201 cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc
     1261 gagcctcatc ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt
     1321 gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ccaaacgaga atgattatca
     1381 aattcattta aatgggttgt ggtgatttct ctgatagacc tggattatga ctacgaaaag
     1441 attgatgact tgaatgttat ggttatgttt aatgttagca catttacata actttcagct
     1501 tccatacaca acatagcaga aatgtatgac agatcactat ttttgaagct tgtcacagga
     1561 cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg
     1621 gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg
     1681 cacttgccgt tgttgcgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta
     1741 tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact
     1801 ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcggat
     1861 ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct
     1921 tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc
     1981 acattcgcgt gttgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg
     2041 gcatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt cagtctgaaa
     2101 actttattgc ctataaagat atcctcggca aaatctcccg caatcgtatg tacaccatgg
     2161 cagcaacggc gatgtccacc gtatctatgt ccatcgttgg tgcatacatg accatgctgg
     2221 agccgaaata tgtcgttgcg gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt
     2281 cgctgatcaa cccttaccgt gttgatgcca gtgaagaaaa cattcagatg tccaacctgc
     2341 acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaggttg
     2401 ccattatcgt tgccgcgatg ctgatcggct ttatcgccct gatcgccgca ctgaacgcac
     2461 tgtttgctac tgtgactggc tggtttggct acagcatctc cttccagggc atcctgggct
     2521 acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg
     2581 gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga
     2641 aaattgcttc cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct
     2701 tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc
     2761 aaggtaacgt ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg
     2821 tgctgtctgc gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa agccggggat
     2881 cattccccgg cttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata
     2941 cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac
     3001 aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat
     3061 catcgcatcc agcgtattcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat
     3121 atcagcctgc aaacgcttta accgttcgta gttggcatat ccggtgccaa agtcatcaat
     3181 cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt
     3241 agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg
     3301 cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa
     3361 attgactgaa aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa
     3421 ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag
     3481 aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata
     3541 accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa
     3601 cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc
     3661 ctctctgcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct
     3721 taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc
     3781 accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt
     3841 aaccatatgc tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact
     3901 acccggcaat tgatacaact tttcgttttc ctgcatcaac ggcagcagcg tacggcaaat
     3961 tgagcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt
     4021 atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc
     4081 acgaaagttg ggtaggagcg ttaacggatc tgtcagcgcc tgcaaatgcc agcggcgatt
     4141 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
     4201 cagcaccgca agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
     4261 gttgtaattc agcaggcaaa gcgttgaaac agcccaggtg agatttaaaa acggatagcg
     4321 aagcttaccg accccgaggg taaagatgat gaagaaaacg ggtaccaagt aaccggcaat
     4381 aaagtcgttt tcataaggtg tgcacaacag aagtagcagc acgcttaacg ctgccagcca
     4441 gcttaaggta aatgcgcgtt tctctttgcc caacgacgga gcgatatccc tgcgccacaa
     4501 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
     4561 aatcagcaca gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc
     4621 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
     4681 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
     4741 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
     4801 caacacgaca aacgtctggc taccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
     4861 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
     4921 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
     4981 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
     5041 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
     5101 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
     5161 gcaaacaggg ggccagcctg ttgccgattt ttcatccaat gatgctttca ccaggcggca
     5221 ctaacccgga cgtagactgt attacaaaag cggcaaaaag cagagacaaa aaacccccgc
     5281 tttgcagcga gggttggaaa tttggtggag ctaagcggga tcgaaccgct gacctcttgc
     5341 atgccatgca agcgctctcc cagctgagct atagccccac gatgcgttta cgtaccaaat
     5401 ttgctgggtg caaaatttgg tggagctaag cgggatcgaa ccgctgacct cttgcatgcc
     5461 atgcaagcgc tctcccagct gagctatagc cccgtcacgt aaagcttgtc gagttgacgg
     5521 gcggcatcat atgaattccg cccgaatgtg tcaacggcaa attgcaacgt gtaatttcaa
     5581 tcgctgaaaa atcaggcaaa tgaacaattt tggaaaaccg ctcgcattca gtagttattc
     5641 atgtcacggt ttcctgtaaa gtggtgttat aaaatgaact actaatagac ccacatacat
     5701 tcagggaatt gttatgttca aggagaggat gacaccagat gaacttgcca gactgaccgg
     5761 ctatagccgc cagaccatta ataaatgggt acgcaaggaa ggctggacaa cgtcaccaaa
     5821 acctggcgtc cagggtggca aagcccgact ggttcacgtc aatgaacagg ttcgtgaata
     5881 tattcgcaat gctgaacgtc cagaaggtca gggagaagcg cctgcccttt ctggtgatgc
     5941 gccgcttgaa gtattgctgg tgacactggc gaaagagatg acgccagttg aacaaaaaca
     6001 gtttacatcc ttgcttctgc gggaagggat tatcggattg ttacaacgct tagggattcg
     6061 cgatagcaaa taatatgaaa agattacgca acaaaatgac caccgaagaa ctggctgaat
     6121 gcctcggtgt ggctaaacaa actgttaacc gttggatcag agaaaaaggc tggaaaacgg
     6181 aaaaatttcc cggcgtgaaa ggcggtcgtg ccagacttat tctggtcgat acgcaagttt
     6241 gcgagtttat tcagaacacg ccagccttcc ataacacacc aatgttgatg gaagctgaag
     6301 agagaattgc tgaatacgct cctggagcac gcgccccggc gtatcgacaa atcatcaacg
     6361 cgatagataa tatgacagac attgagcagg aaaaagtcgc gcaatttttg tcacgtgaag
     6421 gaattcgcaa tttcctcgcc cgtctcgata ttgacgaatc agcataaata aaaaacggca
     6481 ggatattatc tcctgccgtt tatcttttta cacgctaatt actgctgatt ttcgcgttca
     6541 gcaataaaat ccagcgcttt gttgatacgc tcgatactgc gggtcttacc aattgcgtga
     6601 acggtaacat ccagtgctgg agactgcccc gcaccggtta cggcgacacg cagcggcata
     6661 ccaactttac ccatacccac ttccagctca tccgccgtcg cctgaatagc gtgatgaacg
     6721 ttttcagcgg tccagtcagt aatcgcggcc agtttgtcac gaaccacttc cagcggctga
     6781 cgcgctaccg gacgcagatg ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg
     6841 tagaaataac ggcagctctg tgccatctct ttcagcgtct tgcagcgttc gcccagcagt
     6901 ttcaccagat cagccagctg cgggccgtta cgggtatcga tattttcctg ctcaatgtgc
     6961 cactgtaagt gagtagcaac atactccggc ggcagcgcgt taatgtagtg atggttcagc
     7021 cacagcagct tgtcggtgtt gaacgcactg gcagatttgc tgacggcatt caaagtgaag
     7081 tatttgatca tctcttcacg agtgaagatt tcctgatcgc cgtgggacca gcccagacgc
     7141 accagatagt tcagcagtgc ttctggcaaa taaccgtcat cacgatactg cattacgctg
     7201 actgccccgt gacgtttgga cagtttttta ccgtcatcgc cattgatcat agaaacgtgc
     7261 gcgtaaaccg gcaccggcgc tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg
     7321 ttgatatggt cttcgccacg gataacgtgg gtgatttcca tatcccagtc atcgacaacc
     7381 acacagaagt tataggttgg ggaaccatcg gtacggcgga taatcagatc atccagctcc
     7441 tggttgctga actcgatcgg accacggatc tgatcgtcaa aaacaacaga accttcctgc
     7501 ggattagcaa aacgcacgac acacggttca tcatcagcat gatgctcatg gctgtggcgg
     7561 cagcgaccgt cataacgcgg cttctcacct ttcgccattt gctcttcgcg cagcgcttcc
     7621 aggcgctctt tagagcaata gcatttataa gcagtgccct cttccagcat ctgatcgatc
     7681 accgcattgt agcgatcaaa acgtttggtc tggtagtacg gaccttcatc ccactccagg
     7741 ctcagccagt tcatgccatc cataatggct tcgatagctt ccggcgtgga acgctcaaga
     7801 tcggtgtctt caatacgcag cacgaactca ccgccgtggt tacgtgcaaa aagccaggag
     7861 taaagagcag tacgcgcgcc gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga
     7921 gttttgattt tcatgaaatg gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg
     7981 gaaaaattga gtgggcgata ttctatcact cccgcctgat tcctcaatgt agttcgggtt
     8041 tctaccttac gcattattgt tttcgttgac aaattgcgca acacggtctg ttttgcgatc
     8101 atcttgctta aatttacgac gaacgaacaa tttctttaga aaatgcgttg actcattttg
     8161 aactctccct ataatgcgac tccacacagc gggggtgatt agctcagctg ggagagcacc
     8221 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaac tactttatgt
     8281 agtctccgcc gtgtagcaag aaattgagaa gtgggtgatt agctcagctg ggagagcacc
     8341 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccact ttctcgccag
     8401 ctaaatttct tgtaataatg tgaagtaccg aagtgggtga ttagctcagc tgggagagca
     8461 cctcccttac aaggaggggg tcggcggttc gatcccgtca tcacccacca cttcgggtcg
     8521 ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca
     8581 cgacccacca atgtaaaaaa agcgccctaa aggcgctttt ttgctatctg cgatttgtaa
     8641 gattgatttt tcttctcaag ttgcttgttc ttctaccgat attacctcgt acagtgacga
     8701 ggactgaaca atggccacta tctcttcaac ttcaattcct tccatacaaa cacagagtag
     8761 taacagggca tcacaaggta gcgatgttgc ttcacaaatc gcgcggataa gtcagcaaat
     8821 catcaaactc actcaacaaa ttaaagagat tgtagacact agcggcagtg ctgaagacaa
     8881 gcaaaaacag gccgaattga ttcaacagca aatcacccta ctggaaacac aactggcgca
     8941 gttgcagaaa cagcaggcag aaaaggcgca ggagaaagag cagtgtttgt ctctgaatgt
     9001 atcccttcta aatcctgttg aaaaaacgac tcatatcgat atctatatct gatacaacgg
     9061 atccccttcc cgcccagcca ttagcgcatt gccctggtct cgtaccacgt cccataacgc
     9121 ttcagcggcg gtggaaagtg aacggttctt cctgcgcacc agcatcaact ggcgttcaac
     9181 caccggcgta atgcgcttta ccaccagtgg gctaccttca ggcaatggta gcg
//
LOCUS       DAEVZA010000169         8904 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEVZA010000169 DAEVZA010000000
VERSION     DAEVZA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.169"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(1..767)
                     /locus_tag="LAF92_004439"
                     /pseudo
     CDS             complement(1..767)
                     /locus_tag="LAF92_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(767..1384)
                     /gene="hyfA"
                     /locus_tag="LAF92_004440"
     CDS             complement(767..1384)
                     /gene="hyfA"
                     /locus_tag="LAF92_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBI9793350.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(1637..2107)
                     /gene="bcp"
                     /locus_tag="LAF92_004441"
     CDS             complement(1637..2107)
                     /gene="bcp"
                     /locus_tag="LAF92_004441"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBI9793351.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(2107..2679)
                     /locus_tag="LAF92_004442"
     CDS             complement(2107..2679)
                     /locus_tag="LAF92_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBI9793352.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            2825..3703
                     /gene="dapA"
                     /locus_tag="LAF92_004443"
     CDS             2825..3703
                     /gene="dapA"
                     /locus_tag="LAF92_004443"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBI9793353.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            3720..4754
                     /gene="bamC"
                     /locus_tag="LAF92_004444"
     CDS             3720..4754
                     /gene="bamC"
                     /locus_tag="LAF92_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBI9793354.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPVALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            4967..5680
                     /gene="purC"
                     /locus_tag="LAF92_004445"
     CDS             4967..5680
                     /gene="purC"
                     /locus_tag="LAF92_004445"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBI9793355.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            5834..6061
                     /gene="vapB"
                     /locus_tag="LAF92_004446"
     CDS             5834..6061
                     /gene="vapB"
                     /locus_tag="LAF92_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBI9793356.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            6061..6459
                     /gene="vapC"
                     /locus_tag="LAF92_004447"
     CDS             6061..6459
                     /gene="vapC"
                     /locus_tag="LAF92_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBI9793357.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            6606..7469
                     /locus_tag="LAF92_004448"
     CDS             6606..7469
                     /locus_tag="LAF92_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBI9793358.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            7484..>8904
                     /gene="tmcA"
                     /locus_tag="LAF92_004449"
     CDS             7484..>8904
                     /gene="tmcA"
                     /locus_tag="LAF92_004449"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBI9793359.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTG"
BASE COUNT         2207 a         2179 c         2449 g         2069 t
ORIGIN      
        1 cgaaaatacc gattttgacc attacgccag acatcaacgc cgaagcgtgc gatggtgcgg
       61 cagggtgagc gcgcggcaac cagctgtgca acggcatcat cccggctttc gcgccaaaac
      121 cgaaaaaggc cagcaggaac accgccgacg ccagccccgg agaaagtgaa agcgtgcgga
      181 aactggcaaa atcgaggctg ccgctttcgc gccccatcag caagaaggcg atcataatca
      241 gcaccgatcc ggcgtgcgat aaaaaagtag agcatgccag cgttgatcga cgttttatcc
      301 tgcctggcaa tgaccagaaa ccaggaagac agcgacatca tttcaaacag cacgatgaac
      361 caaaaagcgt tgtccatcac cagcagggca accatcgatg cgatgaaaag attcataaag
      421 aagcccatcg ccgccgcgcc tttgccctcg tattcgcgca tataagtcaa tgaatagagc
      481 gaacaaacca ccaccagcaa ggagataacc agcaccataa atgccgagag gctatccatt
      541 cgcagggtta aatcggcaaa gtcaaaaggg gtggcaaaat gggcgactaa agttacacca
      601 gcatgtaatt gcgtaattcc gctgatgatg ccaatcacgc cgcccaccag cgatgtgatg
      661 ccggaaagct taatagccag tctgtccaga ccgagtaaaa acagcgaagc cagactggca
      721 aacagataga ggatcagcga ccaggttaat aattgcaggg catccattag tgttgctcct
      781 gagtgaggga aaggggatcc tccccgccat tggcaaacca gcttgcggca aggcgctgtt
      841 tttctttcat ctgacgttgc aggctatcgt cggtgatcag ccgtaacgcc tgattcgggc
      901 aagcgcgaac gcaggccgga ccttctggca agaaatcaca caggtcgcat ttcaccgcga
      961 cggtctggac acccggttcc cagcgcagca acgcatgttg gggcagcacg ttttcttcgc
     1021 cgtctttgag tgagccttca gcctgaaaaa catattgcgc atgggcattc accggacggc
     1081 ttcctgaagc gctgattgcg ccaaatgggc agaccacggc gcaaagcttg cagccaatac
     1141 agaggctttc gttgagttgg atcgcatcat ccctctgaga gatggcattg accgggcaga
     1201 cctgcaggca aggggcttcc tcacagtgat gacacacgac aggggcagtg attgttgacg
     1261 tcttcgccag ggccaggcgc gggtgttgct gtaaaccttg cgttttatgc acgtccgaac
     1321 aggcagcgag acaggtatta catcctgtac accacagtgg ttcggccacc acaaagcggt
     1381 tcatgtcggc tccttgaaaa tgatatgaaa cattgcattt catattgcat aaaggatgcc
     1441 attctgatta actcaatata ttcaataagt tatgtattga ctggattctg aacggtacaa
     1501 aaatgatggt cattaacgtt cagcaaaaaa tggattcgac acccgtgtcg caggggaaag
     1561 cgagaatgtg atcttgatca cgagaagtga ggtatgctgg ccgcaagcgc agccagcacg
     1621 gaatggagca aagtaatcag gcgtgttctt tcagccagtt cagcacaacg tcgtggtgat
     1681 tgctggtttt gaaatcgtca aagacatgtt cgattttgcc atcagcgtca atcaggaagc
     1741 tgatgcgatg aatgccatcg taggttttgc ccatgaagga cttttcaccc cagacgccga
     1801 attgttcgca cacctggtgg tcctcatcag acaggagcgt aaagttaagc agctcttttt
     1861 ccgcaaaacg ggagagtttt tcgggtttat cggtgctgat acccagcaca tcaacgcccg
     1921 cttttttcaa ctcatccatg ttatcgcgta agccgcaggc ctgtacggta cagccggggg
     1981 tcatggcttt cgggtagaaa taaaccagaa cacgctgtcc ctggaagtcg gtcaaattaa
     2041 cttgttctcc gtcttgatcc ggcaagctaa atttcggtgc gatatcaccg gctttcagtg
     2101 gattcattac ttaactccat cctgttcatc atgttgggaa taattgacga cgttaatact
     2161 gccttgtgca ttgagttctg tacatagggc tttgaacgct tgctcaatat ttgctgcgtc
     2221 cgcagatgcg gggctgtggg cggttatctg aatatgcaac tgcgcagccc gttcattttc
     2281 agcaggttgc gtgcgcgaca ccagctccgc aatgttcata tgatgcgcgt cgaaaagtgc
     2341 tgtgaagcgt tcaattaaat gcggggagtc tgccacatcg acctgaaccc agacagatgc
     2401 tggcattggc ggacgcggac gcgccgtcgt gcgcttcatc acgattaaaa gatccagttc
     2461 ggcacctttc aacggtaacg ttgattcaat cagagtaatg gcattccatg aaccggaaag
     2521 cagcataata aacgtgaact cttctcccag catcgccagg cgactgtctt caatattaca
     2581 gccgcaacta ctgacatgac gggtgatggt gttcacaatt ccagggcgat cggcacccaa
     2641 cgcagtgatc accagataat gttgcgatga cagtgtcaaa ctggttattc ctttaagggg
     2701 tgagttgttc ttaaggaaag cataaaaaaa acatgcatac aacaatcaga acggttctgt
     2761 ctgcttgctt ttaatgccat accaaacgta ccattgagac acttgtttgc acagaggatg
     2821 gcccatgttc acgggaagta ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt
     2881 ctgtcgggct agcttgaaaa aactgattga ttatcatgtc gccagcggta cttcggcgat
     2941 cgtttctgtt ggcaccactg gcgagtccgc taccttaaat catgacgaac atgctgatgt
     3001 ggtgatgatg acgctggagc tggctgatgg gcgcattccg gtaattgccg ggaccggcgc
     3061 taacgctact gcggaagcca ttagcctgac gcagcgcttc aatgacagtg gtatcgtcgg
     3121 ctgcctgacg gtaacccctt actacaatcg tccgtcgcaa gaaggtttgt atcagcattt
     3181 caaagccatc gctgagcata ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac
     3241 tggctgcgat ctgctcccgg aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg
     3301 aatcaaagag gcaacaggga acttaacgcg tgtaaaccag atcaaagagc tggtttcaga
     3361 tgattttgtt ctgctgagcg gcgatgatgc gagcgcgctg gacttcatgc aattgggcgg
     3421 tcatggggtt atttccgtta cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa
     3481 actggcagca gaagggcatt ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt
     3541 acacaacaaa ctatttgtcg aacccaatcc aatcccggtg aaatgggcat gtaaggaact
     3601 gggtcttgtg gcgaccgata cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg
     3661 tgagacggtc agagcggcgc ttaagcatgc cggtttgctg taaagtttag ggagatttga
     3721 tggcttactc tgttcaaaag tcgcgcctgg caaaggttgc gggtgtttcg cttgttttat
     3781 tactcgctgc ctgtagttct gactcacgct ataagcgtca ggtcagtggt gatgaagcct
     3841 acctggaagc ggcaccgctt gcggagcttc atgccccggc tggaatgatt ttgccggtga
     3901 cctccggtga ttatgcaatc ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg
     3961 acattcgtcc accagcccag ccgctggcac tggtttctgg cgcgcgtacc cagttcacgg
     4021 gcgataccgc ttcattgctg gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg
     4081 ttagcgtgct gcaggcgaaa aactacacca tcacccaacg tgatgatgct ggtcagacac
     4141 tgaccaccga ttgggtacaa tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt
     4201 atcaaatctc tgttaagccg cagggttatc agcaggcggt tacggttaaa ctgctgaacc
     4261 tggaacaggc gggcaaaccg gttgcagacg cggcttccat gcagcgttac agcacggaga
     4321 tgatgaacgt tatttccgcc ggtctggata aatctgccac tgacgccgcg aacgctgcgc
     4381 aaaatcgtgc ctccaccact atggacgtac aaagtgcagc tgatgacacc ggtttaccaa
     4441 tgctggtcgt acgcgggccg ttcaatgtgg tttggcaacg tctgccagtg gcgctggaaa
     4501 aagtgggcat gaaagtgacc gacagcaccc gttcgcaggg caacatggcc gtaacttata
     4561 agccgctgtc tgacagcgac tggcaggaac tgggcgcgag cgatccaggc ctggcatccg
     4621 gtgactataa actgcaggtt ggcgatttag ataaccgcag cagcctacag ttcatcgatc
     4681 cgaaaggtca tactctgact cagagtcaga acgacgcgct ggtagctgtc ttccaggctg
     4741 cgtttagcaa gtaaaaatac agggctggag tcatccggcc cttttttctg atatgatacg
     4801 caaacgtgtg cgtcggcagg aaaacgcgat tttagcggta attcgcacga aatttgtttg
     4861 tcggacgtag ttcggataag gcgttcacgc cgcatccgac aaaacatccg gcacaccaga
     4921 cagcaaaaga ttttaaaacg ttaattcaca cccaggagtg ataaagatgc aaaagcaagc
     4981 tgagttgtat cgtggtaaag cgaaaaccgt atacagcacg gaaaacccgg acctgttggt
     5041 gctcgaattc cgcaatgata cgtcagcagg ggatggcgcg cgcattgagc agtttgatcg
     5101 caaaggtatg gtgaacaaca agttcaacta cttcattatg agcaaactgg ctgaagcggg
     5161 tatcccgact caaatggagc gtctgctctc cgataccgaa tgtctggtga aaaagctgga
     5221 tatggtgccg gttgagtgtg tcgtgcgtaa ccgtgctgct ggctctctgg tgaaacgtct
     5281 tggaatcgaa gaaggtattg agctgaaccc gccgctgttc gatctgttcc tgaaaaacga
     5341 tgccatgcac gatccgatgg tcaacgaatc ttactgcgaa acctttggct gggtgagcaa
     5401 agagaacctg gcgcgtatga aagagctgac ctacaaagcg aacgacgtgc tgaaaaaact
     5461 gttcgatgat gctggtctga ttctggtcga cttcaagctg gagtttggtc tgtacaaagg
     5521 tgaagtggta ctgggcgatg agttctcccc ggacggcagc cgcctgtggg acaaagaaac
     5581 gctggagaaa atggacaaag accgtttccg ccagagcctc ggtggcctga tcgaggccta
     5641 tgaagccgtc gcccgccgcc tgggtgtaca gctggactga tttttctggt tgcttgttta
     5701 tttagccgga gggtactccg gctaaatgtc tttgataccc ccgctacaat acttctcatt
     5761 atctcccttt gacaggaaag tcataccagg ccaatatgta tatacataac gtatatccca
     5821 aaaagggggg gctatggaaa ctactgtatt tttaagtaat cgcagtcagg ccgtcagatt
     5881 accgaaagcg gtcgctctgc cagaagacgt taagaaagta gaaattattg cgattggtcg
     5941 tacaagaatc atcactcccg ccggtgagtc ctgggacagt tggtttgatg gcgaaaatgt
     6001 gagtgcggat tttatggata tccgtgatca acccgcaatg caggaaaggg aatcattttg
     6061 atgctgaaat tcatgctgga taccaatatc tgtattttca ctattaagaa taaaccagta
     6121 catgtcagag agcggtttaa cctgaattct ggccggatgt gtataagttc tgtaacattg
     6181 gtggagctta tctatggggc agaaaaaagt cagatgccag aacgcaattt ggcggttatt
     6241 gaaggtttcg tatctcgttt ggtagtgctt gattatgata gtgctgctgc gacgcatacc
     6301 gggcaaatca gggccgagct tgcccggcag ggaaggcctg ttggaccttt tgaccagatg
     6361 atagcgggac atgctcgcag tcgcggtttg attgtggtaa ccaacaatac ccgagagttt
     6421 gagcgagtgg cgggaatcag actagaagac tggagctaac attctctttt acttctaaca
     6481 ttcgtaaatt atataacgtg gcatgaagcc ttaattcctc cgccatttca tcccgctttc
     6541 ctgtggtaat cttcctctga accacctacg atcatttcta taatgaatat attgttgagg
     6601 tatctatgcg ttggcaaggg cgacgtgaaa gtgacaatgt tgaagacagg cgcaacagct
     6661 ccggtggtcc gtctatgggc ggtcctggtt ttcgcctgcc aagcggtaaa ggcgggctga
     6721 ttttactgtt agtcgtgctg gttgcaggct actatggtgt tgatttaacc gggttaatga
     6781 ctggacagcc ggtttcccaa caacaatcaa cgcggtccat tagccctaat gaagacgaag
     6841 ctgcaaaatt cacctcggtg attctggcaa ccacggaaga cacctgggga caacagttcg
     6901 agaagatggg taagacctat cagcaaccga agctggtcat gtaccgtgga atgacgcgca
     6961 ccggctgcgg ggctggtcag tccattatgg ggccgttcta ttgcccggcg gatggcacag
     7021 tttatatcga tctctccttc tatgatgaca tgaaagacaa acttggcgcg gatggcgatt
     7081 ttgcccaggg gtacgttatc gcccatgaag tcggtcatca tgtgcagaaa ctgttaggca
     7141 tcgagccgaa agttcgtcaa ctgcaacaaa acgcgacgca ggcggaagtg aaccgcttat
     7201 ctgtgcgtat ggaactccag gccgactgtt ttgccggtgt ctgggggcat aacatgcagc
     7261 agcaaggcgt tctggaaacc ggcgatctgg aagaggcgct gaacgcggcg caggccatcg
     7321 gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagatagt ttcacacatg
     7381 gcacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc gatccggcac
     7441 aatgcaatac ttttggtaaa agcatttaac ttccggggca gggatggctg aactgactgc
     7501 gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag
     7561 cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct tacctggcga
     7621 ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttcaccct cggcgctaca
     7681 aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg gctttgatgc
     7741 cgctgccttt gcagcactta gcggaacgtt gaaagcggga agctggctgg ttttgttact
     7801 ccctgtatgg gaagagtggg aaaaccaacc tgatgccgat tcgctgcgct ggagtgattg
     7861 ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac ttacggcgga
     7921 taacgacgct atcctctggc ggcaaaacca gccgttctcg ttggcgcatt ttactccccg
     7981 tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac tcttacagca
     8041 gctactgacc atgcctccgg gcgtggcagc ggtaacggct gcgcgtgggc gcggtaagtc
     8101 ggcgttggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc
     8161 aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct ttattgcacc
     8221 ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgacg aagccgcagc
     8281 catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt tgttaaccac
     8341 tacggtgcag ggctacgaag gcaccggacg tggttttttg ctgaaatttt gcgctcgctt
     8401 tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg gatgcccgct
     8461 ggaaaaaatg gttagtgagg cactggtttt tgacgatgaa aacttcaccc atacaccaca
     8521 aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgagc cagaaacgcc
     8581 gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acttcgccgc tggatttacg
     8641 ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa acgagattgc
     8701 cggagcgctg tggctggtgg atgagggggg attatctcaa caactcagtc aggcggtatg
     8761 ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg cgcacggtag
     8821 caatccactg gcagcgacat tgcgtggacg gcgggtcagc cggatagcag ttcatccggc
     8881 gcgtcagcgg gaaggcacag ggca
//
LOCUS       DAEVZA010000170         8724 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEVZA010000170 DAEVZA010000000
VERSION     DAEVZA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.170"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..567
                     /locus_tag="LAF92_004450"
     CDS             <1..567
                     /locus_tag="LAF92_004450"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="NuoCD; NDH-1 shuttles electrons from NADH, via FMN
                     and iron-sulfur (Fe-S) centers, to quinones in the
                     respiratory chain; subunits NuoCD, E, F, and G constitute
                     the peripheral sector of the complex; in Escherichia coli
                     this gene encodes a fusion protein of NuoC and NuoD that
                     are found separate in other organisms; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBI9793360.1"
                     /translation="AYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENFDFEIPV
                     GGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERTLQHIET
                     LITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSYAHLQQI
                     PAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            570..1070
                     /gene="nuoE"
                     /locus_tag="LAF92_004451"
     CDS             570..1070
                     /gene="nuoE"
                     /locus_tag="LAF92_004451"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBI9793361.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            1067..2404
                     /gene="nuoF"
                     /locus_tag="LAF92_004452"
     CDS             1067..2404
                     /gene="nuoF"
                     /locus_tag="LAF92_004452"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBI9793362.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            2457..5183
                     /gene="nuoG"
                     /locus_tag="LAF92_004453"
     CDS             2457..5183
                     /gene="nuoG"
                     /locus_tag="LAF92_004453"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBI9793363.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            5180..6157
                     /gene="nuoH"
                     /locus_tag="LAF92_004454"
     CDS             5180..6157
                     /gene="nuoH"
                     /locus_tag="LAF92_004454"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBI9793364.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            6172..6714
                     /gene="nuoI"
                     /locus_tag="LAF92_004455"
     CDS             6172..6714
                     /gene="nuoI"
                     /locus_tag="LAF92_004455"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBI9793365.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            6726..7280
                     /gene="nuoJ"
                     /locus_tag="LAF92_004456"
     CDS             6726..7280
                     /gene="nuoJ"
                     /locus_tag="LAF92_004456"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBI9793366.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            7277..7579
                     /gene="nuoK"
                     /locus_tag="LAF92_004457"
     CDS             7277..7579
                     /gene="nuoK"
                     /locus_tag="LAF92_004457"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBI9793367.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            7576..>8724
                     /gene="nuoL"
                     /locus_tag="LAF92_004458"
     CDS             7576..>8724
                     /gene="nuoL"
                     /locus_tag="LAF92_004458"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBI9793368.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGG"
BASE COUNT         1841 a         2303 c         2558 g         2022 t
ORIGIN      
        1 gcctatggcg cgaaagaggc gctggagtgg ggcaccactg gcgcgggcct gcgtgctacc
       61 gggatcgact tcgacgtgcg taaggcgcgt ccttattctg gctatgaaaa cttcgacttt
      121 gaaatcccgg ttggtggtgg tgtttctgac tgctacaccc gcgtaatgct gaaagtggaa
      181 gagctgcgcc agagtctgcg cattcttgag cagtgcctca acaacatgcc ggaaggcccg
      241 ttcaaagcgg atcacccgct gaccacgccg ccgccgaaag agcgcacgct gcaacatatc
      301 gaaaccctga tcacccactt cctgcaagtg tcgtgggggc cggtgatgcc tgccaatgaa
      361 tctttccaga tgattgaggc gaccaagggg atcaacagtt actacctgac cagcgacggt
      421 agcaccatga gttatcgcac tcgtatccgc acgccgagtt atgcgcattt gcagcaaatt
      481 ccggcggcga tccgcggtag cctggtgtct gacctgattg tttatctggg cagtatcgat
      541 tttgttatgt cagatgtgga ccgctaatta tgcacgagaa tcaacaacca caaaccgagg
      601 cttttgagct gagtgcggca gagcgtgaag cgattgagca cgagatgcac cactacgaag
      661 acccgcgtgc ggcgtccatt gaagcgctga aaatcgttca gaagcagcgt ggctgggtgc
      721 cggatggtgc gatccacgcg atcgccgatg tgctgggtat tccggcaagc gacgtcgaag
      781 gtgtggcaac gttctacagc cagatcttcc gccagccggt tggccgccat gtgatccgtt
      841 attgcgacag cgtggtctgt catatcaacg gttaccaggg tattcaggcg gcgctcgaga
      901 aaaagctaaa catcaaacca gggcaaacga catttgacgg tcgctttacg ctgctgccaa
      961 cttgctgcct ggggaactgt gataaagggc caaacatgat gatcgatgag gatactcacg
     1021 cgcatctgac cccggaagcg atccctgaac tgctggagcg gtataaatga aaaacattat
     1081 ccgtactccc gaaacgcatc cgctgacctg gcgtctgcgc gatgacaaac agccagtgtg
     1141 gctggacgaa tatcgcagca aaaacggtta cgaaggcgcg cgtaaggcgc tgaccgggct
     1201 gtctccggac gaaatcgtta atcaggtaaa agacgctggt ctgaaagggc gcggtggcgc
     1261 gggcttttct accggcctga aatggagcct gatgccgaaa gacgaatcca tgaacatccg
     1321 ttacctgctg tgtaacgccg atgaaatgga gccgggcacc tataaagacc gcctgttgat
     1381 ggagcaactg ccgcacctgc tggtggaagg tatgctcatc tccgcgtttg cgctgaaagc
     1441 ttaccgtggc tacatcttcc tgcgtggcga atatatcgaa gcggcagtga atctacgccg
     1501 tgccattgcc gaagccaccg aagcaggtct gcttggcaaa aatattatgg gaacaggttt
     1561 tgacttcgaa ctgttcgtcc ataccggggc agggcgctac atctgcggcg aagaaacagc
     1621 gttaatcaac tccctggaag gacgtcgtgc taacccacgc tcgaaacccc ccttcccggc
     1681 gacctccggc gcatggggta aaccgacctg tgtcaacaac gtcgaaaccc tgtgtaacgt
     1741 tccggcgatc ctcgctaacg gcgtggagtg gtatcagaac atctcgaaaa gtaaagatgc
     1801 tggcaccaag ctgatgggct tctccggtcg ggtgaaaaat ccgggcctgt gggaactgcc
     1861 gttcggtact accgcgcgtg aaatccttga agattacgcc ggtggtatgc gtgacggtct
     1921 gaaattcaaa gcctggcagc caggcggcgc ggggactgat ttcctgactg aagcgcacct
     1981 tgacctgccg atggaattcg aaagtatcgg taaagcggga agccgtctgg gtacggcgct
     2041 ggcgatggcg gttgaccatg agatcaacat ggtgtcgctg gtgcgtaatc tggaagagtt
     2101 tttcgcccgt gagtcctgcg gctggtgtac gccgtgccgc gacggtctgc cgtggagcgt
     2161 gaaaattctg cgtgcgctgg agcgtggtga aggtcagccg ggcgatatcg aaacacttga
     2221 gcaactgtgt cgattcttag gcccgggtaa aactttctgt gcccacgcgc ctggtgcagt
     2281 ggagccgtta cagagcgcca tcaaatattt ccgcgaagaa tttgaggcgg gaatcaaaca
     2341 gccgttcagc aatacccatt tgattaatgg gattcagccg aacctgctga aagagcgctg
     2401 gtaaccgaat ttcgattaac gctcagtctc tgactgagaa aactggaagc atgctaatgg
     2461 ctacaattca tgtagacggc aaagaatacg aggtcaacgg agcggacaac ctgctggaag
     2521 cttgtctgtc tctgggcctt gatattcctt acttttgctg gcatccggcg ctgggaagcg
     2581 tcggtgcttg ccgccagtgt gcggtgaagc aataccaaaa cgcggaagac acgcgtggtc
     2641 gcctggtgat gtcctgtatg acaccggctt ccgatggcac ctttatttcc attgacgacg
     2701 aagaagcgaa acagttccgt gaaagtgtgg tcgagtggtt aatgaccaac cacccgcacg
     2761 actgtccggt atgtgaagaa ggcggtaact gccatcttca ggatatgacc gtgatgaccg
     2821 gacacagctt ccgtcgctac cgtttcacca aacgtaccca ccgtaatcag gatttggggc
     2881 cattcatctc tcacgaaatg aaccgctgca tcgcctgcta ccgctgcgtg cgttactaca
     2941 aagattacgc tgacggtacg gatctgggcg tttacggtgc gcacgacaac gtctacttcg
     3001 gtcgcccgga agacggcacg ctggaaagcg aattttccgg taacctggtc gaaatttgcc
     3061 cgaccggcgt atttaccgac aaaacgcact ccgagcgtta caaccgtaaa tgggatatgc
     3121 agtttgcgcc gagcatctgc cagcaatgtt ctatcggctg taacatcagt cccggcgagc
     3181 gttatggcga actgcgtcgt atcgaaaacc gttacaacgg tacggtaaac cactacttcc
     3241 tctgcgaccg tggtcgtttc ggttacggtt acgtcaacct gaaagatcgt ccgcgtcagc
     3301 cagtacagcg tcgtggcgat gatttcatta ccctcaacgc cgaacaggca atgcagggcg
     3361 cggcagatat tctgcgtcag tcgaagaaag tgatcggtat tggttctccg cgtgccagcg
     3421 tggaaagcaa ctttgcgctg cgtgaactgg tgggcgaaga aaacttctac accggtatcg
     3481 ctcacggtga gcaggaacgt ctgcaactgg cgctgaaagt gctgcgtgaa ggcggcattt
     3541 atactccggc tctgcgcgaa atcgaatctt acgatgcggt actggtgctg ggcgaagacg
     3601 ttacccagac cggcgcgcgc gtcgcgctgg cagtgcgtca ggctgtgaaa ggtaaagcgc
     3661 gcgaaatggc ggcagcacag aaagtggctg actggcagat tgcggcaatc ctcaacatcg
     3721 gtcaacgtgc gaagcatccg ctgtttgtta ccaacgttga tgacacccgt ctggatgata
     3781 tcgcggcgtg gacttaccgc gcaccggttg aagatcaggc gcgtttaggt tttgccatcg
     3841 cccatgcgct ggataactct gcgccagcgg ttgacggtat cgaacctgag ctgcaaagca
     3901 aaatcgacgt catcgtgcag gcactggcag gtgcgaagaa accgttgatt atctccggga
     3961 cgaacgccgg tagcatagag gtgattcagg ctgcggctaa cgtcgcgaaa gccctgaaag
     4021 gtcgcggcgc tgacgttggt atcaccatga ttgcccgttc cgtcaacagc atggggctgg
     4081 gcattatggg tggcggttcg cttgaagaag cgttgaccga actggaaacc ggacgcgccg
     4141 acgcggtggt ggtgctggaa aacgatctgc atcgtcacgc ttccgctacc cgcgtgaatg
     4201 ctgcgctggc taaagcgccg ctggtgatgg tggttgatca tcaacgcaca gcgattatgg
     4261 aaaacgccca tctggtactt tctgctgcca gctttgctga aagcgacggt acggtgatca
     4321 acaacgaagg ccgcgcccaa cgtttcttcc aggtttacga tcctgcttat tacgacagca
     4381 aaactgtcat gctggaaagc tggcgctggt tacactcgct gcacagcacc ctgctgagcc
     4441 gtgaagtgga ctggacgcag ctcgaccatg tgattgacgc tgttgtggcg aaaatcccgg
     4501 aactggcagg tatcaaagat gctgcgccgg atgcgacatt ccgtattcgt gggcagaaac
     4561 tggcccgtga accgcaccgt tacagcggtc gtaccgccat gcgcgccaat atcagcgttc
     4621 atgagccgcg tcagccgcag gatattgaca ccatgttcac cttctcgatg gaaggtaaca
     4681 accagccgac tgcgcaccgt tcgcaagtgc cgtttgcctg ggcgccgggc tggaactccc
     4741 cgcaggcgtg gaacaaattc caggacgaag tgggcggcaa actgcgcttt ggcgatccgg
     4801 gcgtgcgtct gtttgaaacc agcgaaaatg gtctggatta cttcaccagc gtaccggcac
     4861 gcttccagcc gcaggacggg aaatggcgta tcgcgccgta ttaccacctg tttggcagcg
     4921 atgaattgtc acagcgtgct ccggtcttcc agagccgtat gccgcagccg tacatcaaac
     4981 tcaacccagc ggatgccgcg aagttaggtg tgaacgcagg tacacacgtc tcctttagtt
     5041 acgatggcaa cacggtcacg ctgccggttg aaatcgctga agggctgacg gcagggcagg
     5101 tgggcttgcc gatgggtatg tccggtattg ctccggtgct ggctggcgcg catcttgagg
     5161 atctcaagga ggcacaacaa tgagttggat atcaccggaa ctgattgaga tcctgctgac
     5221 catcctcaaa gcggtggtga ttctgctggt ggttgtcacc tgtggggcat tcatgagctt
     5281 tggcgaacgt cgcctgctgg gtctgttcca gaaccgttac ggacctaacc gtgttggctg
     5341 gggcggttcg ctccagctgg ttgcggacat gatcaaaatg ttctttaaag aagactggat
     5401 cccgaaattc tcggatcgcg tcatctttac cctggcaccg atgattgcct ttacctcact
     5461 gctgctggcc tttgcgattg tgcctgtcag cccgggttgg gtggttgccg acctgaacat
     5521 cgggattttg ttcttcctga tgatggcagg tctggcggtt tacgcggtgc tgtttgcggg
     5581 ctggtcaagt aacaacaaat actcgctgct gggtgcgatg cgtgcttctg cgcagaccct
     5641 gagctacgaa gtgttcctcg ggctttccct gatgggcgtg gtggctcagg ccggttcatt
     5701 caacatgacc gacatcgtca acagccaggc gcatgtgtgg aacgtcatcc cgcaattctt
     5761 tggttttatt acctttgcca tcgcgggcgt ggcggtatgt caccgtcacc cgtttgacca
     5821 gccggaagcc gagcaggaac tggcggatgg ttaccacatt gaatattccg gtatgaagtt
     5881 cggtctgttc ttcgtgggtg agtacatcgg gattgtgacc atctctgcat tgatggtgac
     5941 gctgttcttc ggtggctggc aaggcccgtt gttaccgcca ttcatctggt tcgcgctgaa
     6001 aaccgcgttc tttatgatga tgttcatttt gattcgtgcg tcgttaccgc gtccgcgtta
     6061 tgaccaggta atgtccttcg gctggaaaat ctgcctgccg ctgacgctga tcaacttgct
     6121 ggtaacggcg gctgtcattc tctggcaggc gcaataaggg gcaataagac catgacctta
     6181 aaagaattgt tagtaggttt cggcacccag gttcgtagta tctggatgat cggcctgcac
     6241 gcgttcgcca aacgcgaaac gcgaatgtac ccggaagagc cggtctatct gccgccccgt
     6301 tatcgtggtc gtatcgttct gacccgcgac ccggacggcg aagagcgttg cgtagcctgt
     6361 aacctctgcg cggtagcctg cccggtcggc tgtatctcgc tgcaaaaagc agaaaccaaa
     6421 gacggtcgct ggtacccgga atttttccgc atcaacttct cacgctgcat tttctgtggt
     6481 ctgtgcgaag aagcctgtcc gaccacggcg attcagttaa ccccggattt cgaaatgggg
     6541 gaatacaagc gccaggatct ggtttacgaa aaagaggatc tgctgatctc cggtccgggc
     6601 aaatacccgg aatataactt ctaccggatg gcaggtatgg caatcgacgg caaagataag
     6661 ggcgaagcag agaacgaagc caagcctatc gacgtcaaga gcctgttacc gtaaggagag
     6721 gggcaatgga gttcgctttt tatatctgtg gcctgatagc catacttgcg accttgcgag
     6781 tgatcaccca taccaatccg gtacacgcac tgctgtacct gattatttcg ctgctggcga
     6841 tttccggggt gttcttctca ctgggcgctt acttcgccgg tgcgctggaa attatcgtct
     6901 acgcgggtgc cattatggtg ctgttcgtgt tcgtggtgat gatgctcaac ctgggcggtt
     6961 cagaaatcga acaggaacgc cagtggctga aaccgcaggt gtggattggt ccggcaattt
     7021 tgtcggccat catgctggtg gtgattgttt acgccatcct tggtgttaat gatcagggta
     7081 tcgacggtac gccaatcagt gctaaagcag tgggtattac gctgttcggg ccttacgtac
     7141 tggcggtgga actggcttct atgctgctgc tcgcgggtct ggttgtggcc ttccacgtcg
     7201 gtcgtgaaga gcgtgcgggt gaagtgctga gcaatcgtaa agacgacagc gcgaaaagaa
     7261 aaacggagga gcacgcatga tccccttaca acatggactg atcctcgcgg caatcttatt
     7321 cgttcttggc ttaaccggtc tggttatccg tcgcaatctg ctgtttatgc tgattggtct
     7381 ggaaatcatg attaacgcct ccgcgctggc ctttgtggtc gccggaagct actggggtca
     7441 gaccgacggt caggtgatgt acattctcgc catcagcctc gcggcggcag aagcgagtat
     7501 cggccttgcg ctgctgctgc aacttcaccg tcgtcgccag aacctgaaca tcgattcagt
     7561 aagtgagatg cgcggatgaa catgcttgcc ttaaccatta ttttgccatt gattggcttc
     7621 gtcctgctgg cgttctcccg tgggcgctgg tctgaaaacg tttcggcgat cgtcggcgtt
     7681 ggctctgtgg gcctggcggc gctggtaacc gcctttatcg gcgttgattt cttcgctaac
     7741 ggcgagcagg catacagcca gccgctgtgg acgtggatgt cggtaggcga ctttaacatc
     7801 ggttttaacc tggtgctgga cggcctgtcg ctgaccatgc tctcggtggt cactggtgtg
     7861 ggtttcctta ttcacatgta cgcctcctgg tacatgcgcg gtgaagaggg ctactctcgc
     7921 ttcttcgcct acaccaacct gttcatcgcc agcatggtag ttctggttct cgccgataac
     7981 ctgctgctga tgtacctcgg ctgggaaggc gtgggcctgt gctcctatct gctgatcggt
     8041 ttctattaca ccgatccgaa gaatggcgca gcggcaatga aagcgttcgt cgtgacccgt
     8101 gtgggtgacg ttttcctcgc tttcgcactg ttcattcttt acaacgaact gggcaccctg
     8161 aacttccgcg aaatggtgga actggcacca gcgcactttg ctgacggcaa taacatgctg
     8221 atgtgggcga cgctgatgct gctgggcggt gcggtcggta aatctgcgca gttgccgttg
     8281 cagacatggc ttgctgacgc gatggcgggc ccgacgcctg tctccgcgct gatccacgcc
     8341 gcaacgatgg taaccgcggg tgtctacctg atcgcccgta cccacggcct gttcctgatg
     8401 acgccggaag ttctgcatct ggtgggtatt gtcggggcgg ttacgctgct gctggccggt
     8461 tttgccgcgc tggtacagac cgacatcaaa cgtgttctcg cttactctac catgagccag
     8521 attggctaca tgttcctcgc gcttggcgtg caggcatggg atgcggcgat tttccacttg
     8581 atgactcacg cgttctttaa agcgctgctg ttcctggcat ccggttccgt cattctggcc
     8641 tgccatcacg aacagaacat cttcaagatg ggcggtctgc gtaaatctat tccgctggtt
     8701 tatctctgct tcctggtggg cggc
//
LOCUS       DAEVZA010000171         8721 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEVZA010000171 DAEVZA010000000
VERSION     DAEVZA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.171"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..338
                     /gene="envY"
                     /locus_tag="LAF92_004459"
     CDS             <1..338
                     /gene="envY"
                     /locus_tag="LAF92_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBI9793369.1"
                     /translation="LRSSVRDSVCRIIQSDIQHYWNLRIVASSLCLSPSLLKKKLKNE
                     NTSYSQIVTECRMRYAVQMLLMDNKNITQVAQLCGYSSTSYFISVFKAFYGLTPLNYL
                     AKQRQKVMW"
     gene            complement(384..460)
                     /locus_tag="LAF92_004460"
     tRNA            complement(384..460)
                     /locus_tag="LAF92_004460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Arg,seq:tct)
     gene            703..1335
                     /gene="fimZ"
                     /locus_tag="LAF92_004461"
     CDS             703..1335
                     /gene="fimZ"
                     /locus_tag="LAF92_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HBI9793370.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(1338..1853)
                     /gene="sfmF"
                     /locus_tag="LAF92_004462"
     CDS             complement(1338..1853)
                     /gene="sfmF"
                     /locus_tag="LAF92_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HBI9793371.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(1864..2871)
                     /gene="fimH"
                     /locus_tag="LAF92_004463"
     CDS             complement(1864..2871)
                     /gene="fimH"
                     /locus_tag="LAF92_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HBI9793372.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(2884..5493)
                     /locus_tag="LAF92_004464"
     CDS             complement(2884..5493)
                     /locus_tag="LAF92_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBI9793373.1"
                     /translation="MKIPITTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(5524..6216)
                     /gene="fimC"
                     /locus_tag="LAF92_004465"
     CDS             complement(5524..6216)
                     /gene="fimC"
                     /locus_tag="LAF92_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBI9793374.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAQQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(6436..6978)
                     /gene="fimA"
                     /locus_tag="LAF92_004466"
     CDS             complement(6436..6978)
                     /gene="fimA"
                     /locus_tag="LAF92_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBI9793375.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            7459..8325
                     /gene="folD"
                     /locus_tag="LAF92_004467"
     CDS             7459..8325
                     /gene="folD"
                     /locus_tag="LAF92_004467"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBI9793376.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            8327..8539
                     /gene="ybcJ"
                     /locus_tag="LAF92_004468"
     CDS             8327..8539
                     /gene="ybcJ"
                     /locus_tag="LAF92_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBI9793377.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
BASE COUNT         2426 a         2090 c         1931 g         2274 t
ORIGIN      
        1 tcttacgcag cagcgtccgc gacagcgtct gccgcattat tcaaagcgat attcagcatt
       61 actggaatct gcgaattgtc gccagttcgc tatgtttaag ccccagcctg ctcaaaaaga
      121 aattaaaaaa cgaaaatacc agctatagcc agattgtcac agagtgtcgt atgcgttacg
      181 ccgtacagat gttattgatg gataacaaaa atatcactca ggtggcgcaa ttatgtggct
      241 atagcagcac gtcatacttt atctctgttt ttaaggcgtt ttacggcctg acaccgttga
      301 attatctcgc caaacagcga caaaaagtga tgtggtgaag ggcaaagcgg aaacggataa
      361 gacgggcata aatgaagaag aaatggcgcg ccctgcagga ttcgaacctg cggcccacga
      421 cttagaaggt cgttgctcta tccaactgag ctaagggcgc gttgataccg caatgcggtg
      481 taatcgcgtg aattatacgg tcaacccttg ctgagtcaat ggcttttgat ctggttgctg
      541 aacaagtgaa cgaccgcgtc tgattttctg atttatttcg ctatagcggc aaacaaacac
      601 acaccgctgc gcgtctgaat caagaaaacc cgtattttca tgtatcaaag tacaatttac
      661 cgccctaacg gaaaattgtc cgctcctatg agactggtaa ctatgaaacc aacgtcggtg
      721 atcattatgg atactcatcc tatcatcaga atgtctattg aagttctgtt gcaaaaaaac
      781 agtgaattgc agattgtcct gaaaacggat gattaccgca taaccatcga ttatctccga
      841 acccgtcctg ttgatttaat cattatggat atagacttgc ccggaacaga cggttttacc
      901 ttcctgaaaa ggatcaaaca aatccagagc acagtgaaag tgttattttt atcatcgaaa
      961 tcagaatgct tttatgctgg cagagcgata caagctggtg ctaacggttt tgtcagtaaa
     1021 tgcaatgatc agaatgatat ttttcatgcc gttcagatga tcctctccgg atacacgttt
     1081 tttcccagcg aaacgcttaa ctatataaaa agcaataaat gtagtacgaa tagttcaacg
     1141 gtcactgtgc tatctaatcg tgaagtgacc atattacgtt atctggttag cggattatct
     1201 aataaagaaa ttgccgataa gttattactt agcaataaaa cagttagtgc gcataaatct
     1261 aatatttatg gcaagctagg tttgcattca attgtagagc ttatcgacta cgccaaatta
     1321 tacgaattaa tataatatta attataattg atcataaata tcgcatccgc tttcgccaca
     1381 cctggccgaa caccgctggc taacgctcga taattggcaa aaaagtttag tgtgacattg
     1441 ccatttgcat ctacttcctc agtcgggctc gccgctccca gtgcgagccg ggagcgatcg
     1501 ctattacgta attcgatggc gatggtttgc gccattgcgg gatcatccag agccagcagg
     1561 ttggtatcgg atgccggcgt tcccgtaaat aaaatcgcaa ctgaacccgg aggacatccc
     1621 tccagccgca ggctaaaagg gacgagtgcc gtggtatcgc cagcgttcag taattgtgtc
     1681 gtaggccatc tgcctaaatc taccgtctta tcaatatccg cagtgtttac ggtacaggag
     1741 aaatcaacaa cgttaccgtg caaattgata ttaatcgttc ccaaagggtc agctgcccat
     1801 ccactggaaa tccacagtaa cccgcagaaa cagctaaaga gtgctcttct cattctccgt
     1861 acctcattga taatcgacgc gtaaataccc cagcgcgcta aacggccctt cggtcggttt
     1921 ttgaccggta atactgatag gccaggcgcg aagtgtgaca ttggctgccg cagctgcatc
     1981 cagacggaaa ggaataacgc tattgagatc gttaggcgtg atcggcgtat cgttctgatc
     2041 ggcgacaata aaacctaaat cctgattgtc cgacaccatc gcctgaccag aaacggcact
     2101 ggcttccaga cgcattgtta aataagcctg cgcagcaaca ttcgtacatt tgaccgcaat
     2161 gctcttggtt tgcggcatga caccagcagg tcgattaccc ggccctgccg cactaaataa
     2221 cgatgcgccg atatcaccaa aatcaaattc aacaatctgc ccggcattta attcgcagtt
     2281 ttgcggtact tcaacccggc caccaaaact aatggtataa acaggtgtaa ccaacggatc
     2341 gtaaggcgtt gatgtcacat acacggtaaa catcacctgt ctggggatct ccaccatgtt
     2401 aataaaggga cgaataacct ttaaacgaaa tattaaacga gaatccatca ccccgaatgg
     2461 aaggttttgt gaaacgttag gatctgttcc catccgaata taattttgcg gggggaactg
     2521 aatatccatc acactatcaa ccagactcat cgcacctaat agataatcat ttaattgcat
     2581 atatttataa ttatcgatag tttcctgaac aataaagttg gtgacataac tacggtatgt
     2641 ataattcacc agcgtgccgg gtggacaaat tgctgacaca cctacccagc cggatttttc
     2701 cggtaaaaca acaatctgcc ctggctggtt attggtgcta ttaaagatat ttgagagatc
     2761 atagtgttca tcttctgcta cgccagtact atttgcacaa acagtagccc aggatatgtt
     2821 tgccagacac aaacatgcca tcgccaataa taatctgcag attatcttca ttgataattt
     2881 cctttatgag ccaggatgtg tgcaaactgc cgaaataaca gtgacggctt gctgtaagct
     2941 ttgcttcggc aatacatagt gcgcaatgca gcgtgagttc gcgccgtctc cccactgaac
     3001 cagtaattca ccagacaatg gcgcaccact taaataaact tgcccgtcat cacccaccat
     3061 actggttatt ccggtactgt tttcccgtac cagtgatcca aacggcaccg gttttccgcc
     3121 ctgggtaacg gtaatgagcg cccgcacgcc tatgcgggta tcaaaattgg cacgaaccaa
     3181 cgcgccttgc gtcggcacaa cgctgctaat atttttttca acatcgatgg aattccccat
     3241 cgtattggta tcaagcgcga tacggttata ccgataaacc gtggcatacg gcatcaccgc
     3301 atagccgcgc cagtcggtta aaatgccagt ttgattttca atgcgtacac cgcctgcgcc
     3361 aggcgcttta atcaaaacat tggtatcccc taaaggctgg ctcagcgtta tgccattttc
     3421 gtgcccgacc acaccgccag acagctgcca gttaacgtca tgttgatcgc ggtcgtagtt
     3481 atacccgacc cccagcgtac cgtaagtggc ctgccagttt gccgtggcgc tgcccgtata
     3541 cccattattc gaggtatcac cctggcttac gtgataactc aggttgtggc cttccagtaa
     3601 ggtcccacct acacctgcca gccagctatt ttgcccgttg ctgttacggt tggcgttaaa
     3661 ggaggcataa gcgcggtcga gcgcattttc ccgggtgtag cgacgtttgg tcaaaacatt
     3721 gaaaggcact gaaacattaa gtccgacaat acgttcgtta tcggggatcc ctacagattc
     3781 attccacgaa aatgagagcg aataactgat gccaacccag ctgctggtat accccacctg
     3841 ataccaggta tctgaatccg aagtattcca gtatttttga tgggtaccag agatataaag
     3901 cgagccaaag tcattaagtg attgtgaaat atttaactgc aaacggtctt tacggctaaa
     3961 gcgtaaattg tggtaattca cgattatcgg ttcatcgcga tgctcaccgt cgtaatcgta
     4021 atcatattcg tacccctcca ttcgacgata cgcaacatca tcaagggtat aaaaaccttg
     4081 tgtcgaatag cggtaaccca ttaactgaaa attggtgccg aaggtgttca tcgatttcgc
     4141 atagaggaag cgaatggaat ccccctcatg acgactgtcg tcggctaact gactgcgcgc
     4201 atgggtcaca tccagcgaca ctgcccccca gttcccgagg ttgcgcccca gccctaataa
     4261 aaaggcggtg taattggcag ataattgcgt cccgccgtag gcagtaaatt cctgtggtaa
     4321 accgccgagt gccgtgccct gaaaaaagaa aggcgatgat tgctgactat taccgctacg
     4381 aaaatcgccc gccgtcaggt caaatttgaa acgcccttcg cgttgtaaaa ttggcactgt
     4441 tgaatacggg atagtgtaat tctgctgatt accatcgcgc tcgtcgatgg tgacgtccag
     4501 atcgccattt gaagatgtcg ggtgcaaatc tgtaatttca aaagcgccgg gggaaacata
     4561 gctttgatag ataataaaac cattttgccg aatcgttagc tgggccgccg tacgggcaat
     4621 cccacgtacc gttggggcaa acccttgctg gctatcagga tacatattat cggaagaata
     4681 aagccgtaca ccacgaaatc caacaccatc gaaaatatca cttcctgtat tgccgtctcc
     4741 cattaccagt tcacttttca gcggaataat ggcgcgctgt acccaggtgc caatattatt
     4801 ccactgttct gaatgatatc catttccgcg aaaatagttc caggaaccat tgttgcgtaa
     4861 acgccacggg ccaatattaa tcccgctgag ctcactaaaa aaatagctat cgttaccatt
     4921 acctctgtta ccggtgaaat tataattcag gagtaaagca ggaatacctt catcccactc
     4981 ttcaggcgga atgtaaccgt gagcgctact caacaacgtt atctgaggaa tagtgatgtt
     5041 caggcgcatc gctgcaaaat caaagttaat tgtggcatca ggtacagctt tcagtaaatt
     5101 gatgcattta ttgttttgct gctgggctaa ctcgggaaat gcactgctat taaggccaat
     5161 tcgttcaagg agtacctggt taaaacaggg cattaaccca cctgatttat cacctgtatt
     5221 ttctgttgtc gattcaaata cgatatcctg tgaaccaatg aactcatcat tacgccagag
     5281 atccacccga tacactcccg caggttgatg attgcctctc tcaaagcgcg ataaatcagc
     5341 aacggatgtg ccattctcta ataaaaatgc cgggttgaaa taactttccg catagcttaa
     5401 aaaggagggt aaaatggcaa gagatgaata acaaattccg gccagacacc aggtatacct
     5461 ctgcggaata tccgtagtaa tgggtatttt cataattagc tctgatattg tcagatgatt
     5521 cggttagttt aagttcactt ctctgaccgg ggttactgaa ccataatcat taacggtctg
     5581 aaatttcagc tttccctgca ctcctgaggg taagggaatt tgtgaattaa ctctgggtgc
     5641 agccatagca ttccccaact tttggctgcc cacttgtaag ttaaccaggg tgacataaaa
     5701 aggtgaggga ttatgaacat tgatatagtt accggatcgc gaaaacttga gtgtgtccgg
     5761 cgcttctgcg ggtaattctt gtaattgaat tggacggaga aataatttca tgcgcgataa
     5821 aatcgccagt tgcaaaacat tcctgccgtt caatgcattt ttatctaccg aagggatcgt
     5881 cttaacattc atccagaaca gagactcacg atctgccgcc agcggtggac cggtgtaaat
     5941 aatacgcaag gtattttcgc ttttgggttc actaacaaac agtggcggtg taatgatgaa
     6001 tgacttttct tttacaccgc tgctgttttc aatccacgaa ttgaccagaa agcgctcatt
     6061 ggtatggcta tttctaatcc ataccgcagt ctgttgagca tcagcgggat aaataatacg
     6121 cgtggcacct aatgcgatcc ctccggcagc atgggcgctg gccgaaatga ttaaataaaa
     6181 tataatgagc attaataact ttattttagt catcatagtt aatacgcctt gtgtattcct
     6241 gacggcaaaa ataagaaata taaagaaatg attgctttgg tttcttacaa cacatcattc
     6301 ccttttcatt taataaatcg gcatgataca aagtgaacat gatttcgatt tacattgcca
     6361 ccataccgct tcagacaacc ggtacattac tttgttataa gacgtgatat ataattcaaa
     6421 acaacgtggt tttgattatt cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc
     6481 tgctgatgca cttgtaccgg tgcctttata acgtgcagaa aaatgaagaa cgttggtccc
     6541 ggggatcaag ttctggttgg ttgagaagct attcccatca ggtttgagaa ttgcggatgt
     6601 attatcaagt atttcaatac cgacacccgt tgctgttgtt gtattggtac tggatgcaat
     6661 ggccaataaa ttatcattga ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc
     6721 ggcaacaaca ggatcgcagt cgttcaactg aatggtgaat ggaattaatg cagaggtatt
     6781 accaacagca ttgaaaatat cggtacgata ttgaccgagt gtgacaacct ggtctgccga
     6841 gtcagtattc actgaacagg cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc
     6901 atctacaacg gcagcataac tacccgtagc ggcgaatagt gcggcagcca gcgcagacga
     6961 aataaatctt aatttcatat atattccttc aatctcattt atcgactcca catccgtata
     7021 taaccgatta ctttatttaa gacactgata gtagtaaaat tcctttttat cctctaagaa
     7081 tgtcttaatt gaaaatatgc actctattct aaaaaataga gagccccgtt agatgaatac
     7141 ttccgcgcaa aatatattca acaaaaatat agacctgaag cggtaaatta ccaggctgaa
     7201 aattcttttt atattgtcag gtatttctta aattatctta atccttaaac aaggaaataa
     7261 atcagttcca gatttacaac gccatcatgg acgaaaaatg aagctttcaa tctcagcgac
     7321 ctcagcgacg gtgcgcctaa ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga
     7381 aacctgacag cgcgccccgc ttctgacaaa ataggcgcat ccccttcgac ctacgtaaca
     7441 gatggaatcc tctctctgat ggcagcaaag attattgacg gtaaaacgat tgcgcagcag
     7501 gtgcgctctg aagttgctca aaaagttcag gcgcgtattg cagccggact gcgggcacca
     7561 ggactggccg ttgtgctggt gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa
     7621 cgcaaggctt gtgaagaagt cgggttcgtc tcccgctctt atgacctccc ggaaaccacc
     7681 agcgaagcgg agctgctgga gcttatcgat acgctgaatg ccgacaacac catcgatggc
     7741 attctggttc aactgccgtt accggcgggt attgataacg tcaaagtgct ggaacgtatt
     7801 catccggaca aagacgtgga cggtttccat ccttacaacg tcggtcgtct gtgccagcgc
     7861 gcgccgcgtc tgcgtccctg caccccgcgc ggtatcgtca cgctgcttga gcgttacaac
     7921 attgatacct tcggcctcaa cgccgtggtg attggcgcat cgaatatcgt cggtcgccca
     7981 atgagcatgg aactgttgct cgcaggttgc accactaccg tgactcaccg cttcactaaa
     8041 aatctgcgtc atcacgtaga aaatgccgat ctgttgatcg ttgccgttgg caagccaggc
     8101 tttattcccg gtgactggat caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt
     8161 ctggaaaatg gcaaagttgt gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca
     8221 tacattacgc ctgttcccgg cggcgttggc ccgatgacgg ttgccacgct gattgaaaac
     8281 acgctacagg cgtgcgttga atatcatgat ccacaggatg agtaacatgg cgacattttc
     8341 tttaggtaaa catccgcacg ttgagctgtg cgacttgctg aaactggaag gctggagcga
     8401 aagcggcgcg caggcgaaaa tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt
     8461 tgaaacgcgc aaacgctgca aaatcgtcgc cggtcagaca gtgagttttg caggtcacag
     8521 cgtacaggtt gttgcctgat ccctttcccg gcctgtatca acaggccggt tctcttttca
     8581 gacttttgcc ctctacgccc cgttcatcca tgctgatagc atcgacattt tgccgaagcg
     8641 accatcatgc caaccgttat tacgcacgcc gccgttcccc tatgcattgg tttagggttg
     8701 ggttcgaaag tcatccctcc g
//
LOCUS       DAEVZA010000172         8697 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEVZA010000172 DAEVZA010000000
VERSION     DAEVZA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.172"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..216
                     /locus_tag="LAF92_004469"
     CDS             <1..216
                     /locus_tag="LAF92_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793378.1"
                     /translation="NNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGR
                     CPDINKKVQTGDGINNDVDTKTDGTTQ"
     gene            330..1271
                     /gene="zitB"
                     /locus_tag="LAF92_004470"
     CDS             330..1271
                     /gene="zitB"
                     /locus_tag="LAF92_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBI9793379.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(1268..1987)
                     /gene="pnuC"
                     /locus_tag="LAF92_004471"
     CDS             complement(1268..1987)
                     /gene="pnuC"
                     /locus_tag="LAF92_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBI9793380.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2025..3068)
                     /gene="nadA"
                     /locus_tag="LAF92_004472"
     CDS             complement(2025..3068)
                     /gene="nadA"
                     /locus_tag="LAF92_004472"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBI9793381.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(3317..3442)
                     /locus_tag="LAF92_004473"
     ncRNA           complement(3317..3442)
                     /ncRNA_class="other"
                     /locus_tag="LAF92_004473"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(3473..3548)
                     /locus_tag="LAF92_004474"
     tRNA            complement(3473..3548)
                     /locus_tag="LAF92_004474"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3513..3515),aa:Lys,seq:ttt)
     gene            complement(3582..3657)
                     /locus_tag="LAF92_004475"
     tRNA            complement(3582..3657)
                     /locus_tag="LAF92_004475"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3622..3624),aa:Lys,seq:ttt)
     gene            complement(3804..3879)
                     /locus_tag="LAF92_004476"
     tRNA            complement(3804..3879)
                     /locus_tag="LAF92_004476"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3844..3846),aa:Lys,seq:ttt)
     gene            complement(3883..3958)
                     /locus_tag="LAF92_004477"
     tRNA            complement(3883..3958)
                     /locus_tag="LAF92_004477"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3923..3925),aa:Val,seq:tac)
     gene            complement(4108..4183)
                     /locus_tag="LAF92_004478"
     tRNA            complement(4108..4183)
                     /locus_tag="LAF92_004478"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4148..4150),aa:Lys,seq:ttt)
     gene            complement(4186..4261)
                     /locus_tag="LAF92_004479"
     tRNA            complement(4186..4261)
                     /locus_tag="LAF92_004479"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4226..4228),aa:Val,seq:tac)
     gene            complement(4397..4472)
                     /locus_tag="LAF92_004480"
     tRNA            complement(4397..4472)
                     /locus_tag="LAF92_004480"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4437..4439),aa:Lys,seq:ttt)
     gene            complement(4637..5428)
                     /gene="cpoB"
                     /locus_tag="LAF92_004481"
     CDS             complement(4637..5428)
                     /gene="cpoB"
                     /locus_tag="LAF92_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBI9793382.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(5438..5959)
                     /gene="pal"
                     /locus_tag="LAF92_004482"
     CDS             complement(5438..5959)
                     /gene="pal"
                     /locus_tag="LAF92_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBI9793383.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(5994..7286)
                     /gene="tolB"
                     /locus_tag="LAF92_004483"
     CDS             complement(5994..7286)
                     /gene="tolB"
                     /locus_tag="LAF92_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBI9793384.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(7419..8684)
                     /gene="tolA"
                     /locus_tag="LAF92_004484"
     CDS             complement(7419..8684)
                     /gene="tolA"
                     /locus_tag="LAF92_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBI9793385.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
BASE COUNT         2000 a         2317 c         2075 g         2305 t
ORIGIN      
        1 aataacgtcg acaataacgg ggtaaatacc ggttctggtg gcacaatgct gcattcggat
       61 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
      121 aaagatggtc gctgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
      181 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
      241 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
      301 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
      361 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
      421 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
      481 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
      541 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
      601 cgtttgtgaa tgccatagcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
      661 aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat gatggcaatt gccgtggctg
      721 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
      781 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
      841 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
      901 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
      961 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
     1021 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
     1081 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
     1141 agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt cagatggaat
     1201 atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
     1261 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
     1321 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
     1381 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
     1441 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
     1501 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
     1561 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
     1621 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
     1681 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccaaccgt
     1741 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
     1801 agaaaataat gccaaacaag gtgacgttga tcaggccaaa gaagtagttg ctgatcttct
     1861 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
     1921 agagatcata accgcctgcc cctattggta tatgtaccag gatattctgc acactaaaaa
     1981 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
     2041 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctttcatca
     2101 acatgaacct cgtgattgct tccttcctgt tctaatgcct ctgcgatgga ctgaaggcca
     2161 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcaccctc acctgcggtt
     2221 ggtgcttcca gtaactcttt atctggcact gcctgctgca ttttgtagaa aatgccccga
     2281 tcggttgcca cgataagccg ctgatgtggc aatgttttcg cagcagcgat cagttgactg
     2341 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
     2401 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
     2461 tgcacaatac aggcaccctg ccagcataga atgtctccac ccgtctgttt ttgcacgtaa
     2521 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
     2581 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
     2641 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa tgcgttaaat
     2701 tcttcaacag ggcagccgag atccagtgaa cattcagcct gaagtgtcgg catcagaatt
     2761 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
     2821 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
     2881 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
     2941 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
     3001 atgcttaacg gcgtcggctt cgggggaaaa ggataaatcg ccgtgtctgg atcaaacatt
     3061 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaatat
     3121 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
     3181 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
     3241 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
     3301 gggcgctttt ttacattggt gggtcgtgca ggatgactcg cttcgctcgc ccttcgggcc
     3361 gtcgccgcga gcggctccgt tgtttcgctg cgctcaactc aaacctgctg caggttcgaa
     3421 tctttgagta tcgcagatag caaaaaagcg cctttagggc gcttttttac attggtgggt
     3481 cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct
     3541 aacgacccct tgcgggatta tgctgcttta atcattcagg atggtgggtc gtgcaggatt
     3601 cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccata
     3661 cgggtgttac ctgaaagatt tctactcggg gccaatcttt aagattggtg tgtcgtgcag
     3721 gatgactcgc ttcgcttgcc ctacgggccg ttgctggcgc aacgttatcc ttcacgctta
     3781 acatctgagt ttgatgttaa aattggtggg tcgtgcagga ttcgaacctg cgaccaattg
     3841 attaaaagtc aactgctcta ccaactgagc taacgacccg agtggtgggt gatgacggga
     3901 tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct aatcacccga
     3961 tactgcgctg gatactacaa actacttcca ccgaacccac cgtaattagt ggtgggtcgt
     4021 gcaggatgac tcgcttcgct cgccctacgg gccgttgctg acgcaacgtt atcctacacg
     4081 cttaacatct gtcttcgatg ctaaaactgg tgggtcgtgc aggattcgaa cctgcgacca
     4141 attgattaaa agtcaactgc tctaccaact gagctaacga cccggtggtg ggtgatgacg
     4201 ggttcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc
     4261 caatactacg ctggatacta caactaacta tgttagtggt gggtcgtgca ggatgactcg
     4321 acttcgtctc gcccttcggg ccgttgctga tgcaacgtta tccttcacgt tttactaccg
     4381 cttccacctt agcgattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
     4441 gtcaactgct ctaccaactg agctaacgac ccactttttc gttgctttcg gtttgtttga
     4501 tatcccgtgg caacggcggc atatattact gatttcagac ttgagcgcaa caaaaatttc
     4561 gatgcagatc actcaactgc ttatgattcg cacgacacga ccagaaataa tgcgacttct
     4621 ggtcgtgtgt tatgcattac atcgcgttca gacgtttttg tgcctgttta gcgccatcag
     4681 taccagggta tttactgata acctgctggt atacggcttt cgcttttgcg gtgtcacctt
     4741 tgtcctgcat gatgacgccg actttaaaca tcgcatcagc agcctttggt gacttcggat
     4801 agtttttcac taccgaagca aaatagtacg ccgcatcatc ttttttaccc ttgttgtagt
     4861 ttaactgacc cagccaataa ttggcgtttg gcaggtaagt tgaatcaggg taatttttga
     4921 tgaaattctg aaatgccacc attgcgtcat cctggcggga tttatcctgc accagcgcaa
     4981 tagctgcatt gtaatccgtg tttgcatcgc cgcttttcac cggcgcgcca gcattcgcag
     5041 taccagcatc agctgtcggc gtcgttgatg ccgccgcacc gctttgatcg ctgctggttg
     5101 attgcgccgc tgcaccaccg ctgctgaggc tgtcgatctg caacaggatc tgcttttgcc
     5161 gctccacgac ctgattcagt tgatactgat tttcctgaat ctgaccacgc agggaatcaa
     5221 tatcggattg attatcagaa agttgttgct ggagttgggt taaaagctgg ctgtgagcat
     5281 tagaaatacg ctcaagttga gtgacgcggt cttcgaccga gcctgagccg acactactga
     5341 ttggtgcctg agcaaaagcg gcccaggggg ccgctatacc aaccagtaac gacagactca
     5401 atagttgatg tctgaagtta ctgctcatgc aattctctta gtaaaccagt accgcacgac
     5461 ggtttttgga gtatgccgct tcgtcatgac ccagtactgc aggtttttct ttaccgtaag
     5521 aaacgatgga gatctggtct gcagaaacgc ctttaccctg caggtacatc ttaacggcgt
     5581 tcgcacgacg ttcacccagg gagatgttgt attccggagt accacgttcg tccgcgtgac
     5641 cttctacggt gactttgtaa gacgggttgc tacgcaggaa gtttgcatgt gcatccagca
     5701 tttgagcgaa gtcagaacgg atatcgtact tgtccagatc gaagtaaacg atgttgttct
     5761 gctgcagctg ttgcatttgc agacgagcct gctcttcgga agacatgttg ccgttgccgc
     5821 cgttcgcatc cataccagtg ccggcaccca gcatgccttc gctgccgtca ttgctggcgt
     5881 tcttgttgga agaacatgcc gcaattgcca taacaggcag agcaatcatc agccctttca
     5941 gcactttgtt cagttgcatt tcaatgattc ctttactatt caattaatta ttatcacaga
     6001 tacggcgacc aggcagggaa tttgacctgt ccatcagttg ccggaagacg cgctttgaaa
     6061 cgcccatctg tagaaaccaa attcagcacg gatcccatcc cctgagaaga gctgtagatt
     6121 accatagtgc cgttaggtgc cagacttggc gtttcatcca ggaacgtgga cgacagaact
     6181 tgtacgcctc ccgttgccag atcttgtttg gcaatgtgct gctgcccacc attggagctg
     6241 accattacca taaatttacc gtcgctgctg acatccgcat cctggttctg cgaaccttcc
     6301 caggtaatac gttgtggcgc accgccgttg atattcactt tataaacctg cggacgaccg
     6361 gcctggtcag aagtaaatgc caggttctgg ctatccggga accaggtcgg ttcggtattg
     6421 ttactgcgac catcagtcac ctggcggatc tgaccagaag ccaaatccat tacgtacagg
     6481 ttcagactac cggttttcga caaggcgaat gccagtttgc tgccgtctgg cgagaatgca
     6541 ggtgcaccgt tgtgacgcgg gaatgaagcc acctgacgta cagcgccatt tgccagcgtc
     6601 tgaataacca gcgcggaacg accgctttcg aaggtcacat aagccagttt agaaccgtct
     6661 ggtgaccacg ccggagacat cagcggctgc ggtgaacggt gaacgacaaa ctggttgtaa
     6721 ccgtcatagt cagatacgcg cagttcatac gggaactgac cgccgttggt ctgaacaacg
     6781 taggcaatac gggtacggaa cgcaccttta atgccggtca gtttttcaaa cacttcatca
     6841 ctggcggtat gaccagcata acgcagccac tgcttgttca ctttgtacga gttctgagca
     6901 agtacagtac ccggtgcgcc gccagtgtca acaagttgat aagcaacatt gtaagaacca
     6961 tccggattcg gagtgacctg accgacaact acagcgtcaa tgcccagtgc ggaccatgca
     7021 gctggttgta cttcctgcgc actacccggc tgctgtggca gacgagcgcg atctaacgga
     7081 ttaaatttac cgctgttacg caagtcagca gcaacgatgc cgccaatatc ttcaggtgcc
     7141 gcaccaggcc ccgcccactg gaaaggaaca acaccaatag gacgaccgga atctacaccg
     7201 ctgtcgatca caatgcggac ttcagcatgc agaactgatg cccacagtat gagaaaacca
     7261 aatgctactc gtaatgcctg cttcatcata tctcccttat ctggaccgat ggcccacgat
     7321 aatttagcag aatgttaaca aactaaaata cacaaaacta ccagaacccc gtggcaaccg
     7381 gtgcctgatg ttgaccgtcc gaacagtcaa catcgcgatt acggtttgaa gtccaatggc
     7441 gcgtttttga acacttcata tactgcctgg cttggtggtt tcgggatctt cgcaagttta
     7501 gctgctgcca acgcagcctg acaaagtgcg ggatcgccac cttcaggttt gatatccagt
     7561 aacataccat cgggtgccag ttttatgcgc agcgtacagg ttttgcctgc ataggacgat
     7621 gcgtcataga acttactttc gatagcagat ttaatctgcc cggcatagtt attgatatcg
     7681 gcccctgatg cgccattgtt tttagtatta ccactcccgg caggcgaagc attgttccct
     7741 ttcgcccctc cccccgtttt cggtgcattc ttaccagagc ttagctcacc gaaaatatca
     7801 tctgcctctg cggccgcttt tgctgcagcg gctttttcag ctgcagcttt tgccgctgcc
     7861 gcttttttgt ctgcggcggc tttttctgcc gctgctttct tatcagctgc tgccttttca
     7921 gcagccgctt tttctgctgc ttttttgtcg gctgcagctt tctctgctgc cgctttctta
     7981 tcagctgcag ccttttcagc agccgctttc ttctcagctt ctgctttggc tttttcagca
     8041 gcttcagttg ccgctttctt tcttgcttca gctgcagctg cttctgccgc ttccgctttc
     8101 ttcttcagtg ccgcagcggc tgcctcggct tttttctgcg cttcggctgc ggctttggcg
     8161 gcttctgctt ctgctttttt ctttgcgtct gcagccgctt tcttcgctgc ttcttccgca
     8221 gctttagcat ctgcttcggc cttcgcttta gcatctgccg ccgctttcgc tgccgcctct
     8281 tcagcttgct tctgctttaa ctcggcctgt tttgcggctt cttcagcctg ctttttctgc
     8341 tcctgagccg ctaaccgctc tttctcaagt tgcttcaggc gttcctgttc agccgcttgt
     8401 ttctcacgca gttcttcagc agcctgctgt tccttcatct tgcgctgttc atcagaacgc
     8461 ttcgcgcttg attcctggct ttgcatgcgt ttgtactgct caactaccgc acctgaatca
     8521 accatgacag cgtcgatgga cgaaccaccg ccgcctccgg ctgaagcttc tatattctca
     8581 tcgaacgaac tccagatcag cgccgcaaat aagatgacat gcagcactgc tgaaataatt
     8641 atcgcccgct tgagcttgtc gttttgttcg gttgcctttg acactctcgg tttccaa
//
LOCUS       DAEVZA010000173         8575 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEVZA010000173 DAEVZA010000000
VERSION     DAEVZA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.173"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(238..1260)
                     /locus_tag="LAF92_004485"
     CDS             complement(238..1260)
                     /locus_tag="LAF92_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HBI9793386.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
     gene            complement(1245..2810)
                     /locus_tag="LAF92_004486"
     CDS             complement(1245..2810)
                     /locus_tag="LAF92_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9793387.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            complement(2885..3301)
                     /locus_tag="LAF92_004487"
     CDS             complement(2885..3301)
                     /locus_tag="LAF92_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HBI9793388.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            complement(3298..3528)
                     /locus_tag="LAF92_004488"
     CDS             complement(3298..3528)
                     /locus_tag="LAF92_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBI9793389.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            3909..7703
                     /locus_tag="LAF92_004489"
     CDS             3909..7703
                     /locus_tag="LAF92_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HBI9793390.1"
                     /translation="MPVLSANKAQEEQINTSYEPLTPSVITDEKAQSYIEALNFACSR
                     PDIRNIAVTGPYGAGKSSVLLTWERAEDNDFRVMTVSLADFEMQRAYPGDSLSVEGKP
                     DYDDNEKKAGKAEEKTIEYSILQQLLYKEKKSVLPYSRLERISDVSACQIAMMTASLL
                     FILASTATGLLFLFPDYIREKLSLSPELSQFLLELPVLARFGSAGIFLFTALFFALKK
                     LHRTGVFDRRVSIDKVDILKGAITTRPAAPSLLNVYIDEIVYFFEQTQYNVVIFEDLD
                     RHNDGAIFIKLREINQLINNCLPTDNPVRFIYAVRDNLFITPESRTKFFDFVIPVIPV
                     MDSENASEHFLSKFTLDELRQEGFKDCLARLALFIPDMRVMHNIANEFRLYRNIVNNG
                     GDLKRLISLITYKNLYAEDYHRIDQKKGMLYSIVSEYTSGKLREDYCNNLKNKIETCL
                     TELSKLHNEEVVTEHELRSEILRPYISEKTAPRLHIMTGTGGQYDFDDVIQNENTFLS
                     LLSNQIISIKPSGYNITITTIDKKTAQVMKEEYQERIAIIQKKLNNDVSRLEENIKKN
                     RCEIQNASSYDLAFFINKMGRSGFERYIACCSTPEQHDGESITDNAANIDFIYFLLSH
                     GYLSTDYMAYRSVFMPGSLSTEDNNFIRAVTSGRLPDETAKMPLSNIANTVAKLHGLG
                     ILMHDNAWHPQILWYLMRNDTNSLKTIMRMQAEVGAERRMVRLANEIFPLWEPAAQRE
                     YIRLMVDGDGHLSTMIHQIGRLNDTVAEQNLLPVLLSLPILSWEAVSQITREELQRLI
                     DLQFNLVTSLPENCAQFFCENLRNSGCRLTNIPLARSDSGQETLHLVVQKKLWTYSTL
                     NLQNICFSLSHESDNNSDTFRKKPVALIKSLRIPNLEKYVYENISSFIRDVFIHSEEN
                     DLIPDFLNSTFVDWDDAKYMTESMSFVLEDVSVILNKENTETTEISYDQNLYSLLAHH
                     NHITPCWNNVISLLSEDASIAGDTFCEWLNINYSLLPNDSLPLTDVQFSQLLIKAVTS
                     PHISKEALIAITMAFRITLINVPENLPLNNAAVLIKQKWLAPTSTVFEQLYQALYEEG
                     DKLTSLLYALICARPVLLSDNYELVLFSDDQFDLGITRLILNGDKIADEVCISILNWL
                     WEKDEALLSEAPLLSQQALIRFSTKITDDRQKQALLMQCLKNDGGSHKFIRQVLMTFG
                     HQDYAAFLTERNYRSIPRSDAMWQLAVQLGNSGFIRPPKLTHADTRIRIEPFFNAENE
                     YD"
     gene            complement(7748..8164)
                     /locus_tag="LAF92_004490"
     CDS             complement(7748..8164)
                     /locus_tag="LAF92_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HBI9793391.1"
                     /translation="MNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRAAVDATTEVKVALRLAGTPIGPN
                     DTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG"
     gene            complement(8161..8391)
                     /locus_tag="LAF92_004491"
     CDS             complement(8161..8391)
                     /locus_tag="LAF92_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBI9793392.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLR
                     PSWSSFLELEKADPGFMTEREDVVCDEGRFNL"
BASE COUNT         2227 a         2063 c         2046 g         2239 t
ORIGIN      
        1 cgcttcctgg ggggttaatg gctgatgacg ataaaatcat atatcgaatg atttatttct
       61 ctgcctgtaa atatcaggcc aggatttatt atgcgattaa tgataaatat catatcatct
      121 gcgggtttcc ctccacgcag gtccggtttc cggcgaaacc ggagcctcat ttttcggcct
      181 gaagttttgt cgttgactac tttttaccgg cttgtgccca gttgagaagc cgctgactta
      241 gtttctgaat ctccgaagat ggctttccgg aataaaatag atgttgcccc gtgcccgtga
      301 gcaggccacg tatagcctgt tacgggatga tggtggaagt gctgccagct tctgttggat
      361 gaggcgcatc cagtggttgg cccccatcac gatgcagacg tcctgaaagt ggtcgagtcc
      421 tttactgccc ccccagttca ttgagtggca tccgtaacgg tgatgttcgc gatagaagag
      481 cttgatcatc gtgttgtccg cgtgcagggc cgctgaacgc gcttcatccg tgacgatttc
      541 aatctgggtg gtatgtgttc catgagccga aatgcgaatg ttcagttgtc cggtaatgaa
      601 ctcacacact gtagctgaac agcgccatgt ccggctgagc gtctcacaat ccaccatgat
      661 acccgcagcc ctgaagcgag cctcgtatcg ggttatgtct tcatgcagcg tagcattgac
      721 gttaccgtcg cggcttgtgt caaaggtatg ctgataaaaa tccccgcagc acagcactga
      781 gatttccgcg cggcatagct ccagcagaaa attaaagtca tggccggcaa agtcctggac
      841 ttcatcaacg aacaactcat cgtagtaacg ggcaagtcgg gtacggatat ctggcagcaa
      901 cccccgggct gtcagcaggt gtgcgagccg ccggtgatac agccgtccgg cgggatcctg
      961 ataatgccgg atattcgtgc gcggtattct ggaagggggc tggttgaagc tcagaccacg
     1021 cgatgacagt tgctcctgca ggaaagggcg aaaacagaaa ccgtgcagaa actcaaacca
     1081 ggtcatcaca cggatcccgt acggtatgta gccaaagcgt ctgataatct gcgcccgcag
     1141 atgtgcttca ttatttacgg tgaaggtcag gatcagcgtc cgccggtcct ctcttaaccg
     1201 acgtatcagc agcgtggttt tacccgagcc ggcaaccgca aatatcactc tcttatccat
     1261 gccagtgcct cctggatata atcaggaacg gtcagctttt cggcgtgcaa ctgtaacagc
     1321 tggaatgctg cttctgcctt attggccagc atgtaatcct gaaccgtgag tgtccggcgg
     1381 gttccgcgga aaagcgcatc acacaggtcg gcattatcct gatacaggca gatttcaaat
     1441 gtggagcggc tgttgtcgtg gtcagcaaat acccgggagc gggaacaaag cacgtcagca
     1501 tagcgttcat cgcaattttg ctgatagttg ccgtcattgt cgcgaagggc cgcgacacga
     1561 ttttccagaa gacgggccag ttcaagataa cgacggaaac tcgttccgcc gatagcgatg
     1621 atgtgaaccc cgtcatcctc cggtgccctg ccatacaggc ggtgataaaa ggcctcgatc
     1681 agaataaact cagcatcccc ctccacgagc agcacgcgtc tggccagcgc aaattccagc
     1741 acgttattat ccggtgcttt catgaagaag gcggcagtct cagcggaaag ttcattcatc
     1801 agtaccggcc gggtagctcc gaggagaata gccttacgta ggtcgaggcg ggaggagata
     1861 tggctactgt gtgtggcaat aaacacctgc gtctgccgtt ccgtggcaag ctgattaacc
     1921 agcctcttca tactgacatg gctgagatga ttctccggct cctcaagcaa cagcgcgtgg
     1981 atctctcccc cctgctgctg atgccgctgc agcgcaaact ccgttttgat gaaacactgt
     2041 cttccttttc cgcggtgacg aatggaaata ccgtcctcag taatgtccag gttcgcctca
     2101 agacctgatt tagcgccgga acgcacgcca aactgatacg ttttcagcgt gtcgtttatg
     2161 gcggacagat gccgggcgca gaagtgcatt ttctgttgcc ggtaactgtt ttcaagccgg
     2221 taccgatcgg ccaccggcac attgacgctg taaactgtgc gcgtatactc ctgtgcggca
     2281 tgctcgttat caatgcgtgc actgtctaag aacagatggc gcagatagcg tctgaagcct
     2341 gcgaaatgac caccggagaa tgtgctgaac tgcacggaat agtattcata gggaaaatta
     2401 tcaggatcct gctgcaaaac atggtgtata tcctgaccgt attcctccat catgggagca
     2461 atgcgcattc tgagaccatc cgcatcgata cccgccagat tttgcctgcc gttcaggtca
     2521 ggctctcccc cattactcag gaaaacgtca gcggtcagca cgggtaactg atcggcacgg
     2581 cgctcacctt cctgaaaatg cctgactgct gactgtgaca ggagcgattc cacccccagc
     2641 gcctcaaccc gatgcctgct gtcactcagc acaaggtcaa gagccagcag aatagtgctt
     2701 ttcccggact cattgtcccc aaccagaatg ttacgatcgt tgctgaagcg caggtccagt
     2761 tccggaaact ttttgaagtt ttgcagcata agccgcgtga tcagtggcat ttttttattc
     2821 ctttatatac cgctgcctga aagagcggcc ggccgcgcct gaccgcctgt tttgttttac
     2881 agcgttatct tacccagtct tccagcacaa gacccggtac ccgctcaaac tctctcacat
     2941 tattcgtcac cagaatggca caggcagcga tggcgtgtcc ggctatcgcc gtgtcgttcg
     3001 gaccgatcgg cgtgccggcg agacgcagtg ccaccttaat ctccgtggtc gcatccaccg
     3061 cggcgcggtc ccacggcagg acggcatcga gcctctcaca gaacgcgtca accagttgca
     3121 cgtggcgtgg cgaggccttc ggaccggtgg caccgaagcg catctcggag taggtgatgg
     3181 ccgagaccac gatacggtga ccgcgcagca ccgactgctc caggtgctta agcaatgctt
     3241 caggttgctc gcgcatgatg aacgagcaga tgttcgtgtc gagcatatag atcttgttca
     3301 caggttaact cgtccttcgt cgctgacaac gtcctcgcgc tccgtcataa agtccggatc
     3361 ggccttttct agctccagga atgagcccca ggtcggccgg acggggcgca gaatgatgct
     3421 gtccccttcc cggacgatct ccagctcgct caccccctca aaatccagat cgcgtggaag
     3481 gcggatagca cggttgttgc catttttaaa aatggatacg gttctcatga ttctctcccg
     3541 ggtaagtgtg gatatgtgat gcatatccgg aatgatgttc attgcaccgg gtgtgtgcat
     3601 atgtttagta taggcagatg ttaggcatat gtatagattc agcatatgca gatggtgagg
     3661 atatgcgtat accatgaata tgcagatgtc aggcatatgg atatgttcag cctatgttat
     3721 tttctctctt tccccgtgca gatccggttt ctggtgaagt cggagcccac catctccgag
     3781 tatttgataa gccaattctt gtatcccttt gcctttatta tctttttggt tttcagaagt
     3841 tataatgcct gttaaatcaa caatgttgga agatcaacat gagaagacca tcccgaactg
     3901 aagcactgat gccagtactg agtgctaaca aagctcagga agaacagatt aacacctcct
     3961 atgaaccact gactccctca gtcattacag atgaaaaagc acaaagctat atcgaagctt
     4021 tgaattttgc ctgttctcgc ccggatatca gaaatattgc agtaacaggg ccgtacgggg
     4081 ccggtaaaag ttcggtatta ctgacatggg aaagggcgga ggataatgac tttcgggtga
     4141 tgaccgtatc actggctgac tttgaaatgc agcgggcata ccccggagat tcgctgtcag
     4201 ttgagggtaa gcccgactat gatgataacg aaaaaaaagc gggaaaagcc gaagagaaaa
     4261 caatcgagta cagcatcctg caacaactgc tctataaaga gaaaaaaagt gtgttaccct
     4321 attcccgtct ggagcgcatt tctgacgtaa gtgcgtgtca gatagccatg atgacggcca
     4381 gtctgttatt catactggct tcgacggcaa ctggtctgct ttttcttttc ccggattaca
     4441 tccgggaaaa gctctccctg tcgccagaac tgagtcagtt tctgctggag ttgcctgtac
     4501 tcgctaggtt tggtagcgcc gggatatttc tttttactgc tttattcttt gcactgaaga
     4561 aattgcaccg tacaggggta tttgatcggc gtgtaagtat tgataaagtt gacatactga
     4621 aaggtgccat tacaacacgc cctgcggccc cttctcttct caatgtgtac attgatgaaa
     4681 tcgtctactt tttcgaacag acacaataca atgtagtcat atttgaggat cttgaccgtc
     4741 ataatgacgg agccattttt atcaaactcc gggaaataaa tcagcttatc aataactgcc
     4801 tccccacaga caatcctgtc cgatttatct acgctgtcag ggataatctt ttcataacac
     4861 cagaatcacg gacaaaattt ttcgatttcg tcataccagt aatcccagta atggacagtg
     4921 agaatgcttc tgagcatttt ctaagtaaat ttacacttga tgaactcagg caggaaggat
     4981 tcaaagactg ccttgccaga ctggctcttt ttattcctga tatgcgggtt atgcataaca
     5041 ttgcgaatga atttcgtctt taccggaata ttgtgaataa tggaggagat ttaaagcgtc
     5101 ttatttcact gatcacctat aaaaaccttt atgctgaaga ctatcacagg atagatcaga
     5161 aaaaaggaat gctttatagc attgtatctg aatatacatc aggtaaatta agagaggatt
     5221 attgcaataa tctaaaaaac aaaattgaaa cgtgtctaac tgaactatct aaactacata
     5281 atgaagaagt ggtcactgaa catgagttaa gaagtgaaat tttgcggcca tatatcagtg
     5341 aaaaaacagc accccgatta catattatga caggtacagg agggcaatat gattttgatg
     5401 atgtaatcca aaatgaaaat acatttcttt ctttgctgag caaccaaata atcagcatta
     5461 aacctagtgg atataacatt actataacaa caatcgataa aaaaacagct caggttatga
     5521 aggaggaata tcaggaaaga atagcgatta ttcaaaagaa actaaataat gatgtttctc
     5581 gattggagga aaatattaaa aaaaacaggt gcgagattca gaatgcatcc tcgtatgatc
     5641 ttgctttttt catcaataaa atggggagat caggtttcga aagatatatt gcatgttgtt
     5701 caacgcctga acaacatgac ggagaatcca ttactgataa tgccgcaaat attgatttta
     5761 tttatttcct tctatctcat ggttatcttt ccactgacta tatggcctac cgctcagtct
     5821 ttatgccagg tagtctgagc acggaagaca ataactttat cagggctgtg acgtcaggaa
     5881 gattacctga tgaaacggca aaaatgcccc tcagcaatat tgctaacaca gtagcaaaat
     5941 tgcatggact gggcatactg atgcatgaca atgcatggca tcctcagatt ctctggtacc
     6001 tcatgcgcaa tgatacgaac tctctgaaga ctatcatgag gatgcaggca gaggtcggag
     6061 ccgaacggcg gatggtaaga ctggccaatg agatcttccc cctatgggaa cctgctgcgc
     6121 agcgggaata tatcagatta atggtcgacg gggatggtca tttatccact atgatccacc
     6181 agattgggcg cctgaatgat accgtagctg aacaaaatct tctcccggta ttactgtcct
     6241 taccgatact ttcttgggaa gccgtctctc agataactcg ggaagaactg cagagactca
     6301 tcgatttgca atttaatctc gtaacgtcct taccggagaa ttgtgcgcag tttttctgtg
     6361 aaaacctgcg gaattcaggt tgtcgcctga ctaacattcc tttggcccgg agtgactctg
     6421 ggcaggaaac attgcatttg gtggtacaga aaaaattgtg gacctattcc acgttaaacc
     6481 tgcaaaacat ttgtttctca ctttctcatg agtcagataa taacagtgat accttccgta
     6541 agaagcctgt tgctttaata aaatcgcttc gtattccgaa ccttgaaaaa tatgtttatg
     6601 aaaacatatc atcattcatt cgtgatgttt ttatacactc tgaagaaaat gatctcattc
     6661 ctgattttct taattcgaca ttcgttgact gggatgatgc aaagtacatg acagaaagta
     6721 tgagcttcgt actggaagac gtatcagtta tcctgaacaa agagaatacc gaaacaacag
     6781 aaatttctta tgatcaaaac ctgtattcac ttctggctca tcataatcat attacccctt
     6841 gctggaataa tgttatttct ctactgagtg aagatgcgtc aattgctggt gatacttttt
     6901 gtgagtggct taatattaat tattctttat tgccaaatga ctcgttgcct ttgactgatg
     6961 ttcagttctc gcaattactt attaaggctg ttacatcacc tcatatcagt aaagaagcgt
     7021 taatagcgat aaccatggca tttaggataa ctcttattaa tgtgccagaa aacttaccac
     7081 tgaataatgc tgcggtacta atcaagcaaa aatggctggc accaaccagt actgtgtttg
     7141 aacaacttta tcaggcactg tacgaagaag gtgacaaact gacatcacta ctttatgcac
     7201 ttatttgtgc ccgtcctgta ttattgagtg ataattacga gcttgtttta ttttctgatg
     7261 accagtttga cctggggatt actcgtttaa ttctgaatgg cgataagatt gcagatgaag
     7321 tctgtatcag tatcctgaac tggctctggg aaaaagatga agccctgctc tctgaggcac
     7381 cacttctcag ccagcaagcc ctgataaggt tctccaccaa aataaccgat gaccggcaga
     7441 agcaggcact gctgatgcaa tgtcttaaaa atgacggtgg gtcacacaag ttcatcagac
     7501 aggtgctcat gacgttcggg catcaggatt atgccgcttt cctcacagag aggaattatc
     7561 gtagcattcc ccgtagtgat gccatgtggc aactggctgt ccagctggga aatagtggat
     7621 ttatcaggcc gccgaaactt actcacgctg atacacgtat tcgtatagag ccctttttta
     7681 acgctgaaaa tgaatacgac tgacatcgca aaatagtggt aagtccgtcg ggggcggcct
     7741 tactccttta tcctgcccag tcttccagta ccagtccagg cacccgctca aactctctca
     7801 cattattcgt caccagtatt gccccggtgg cgatggcgtg cccggcgatc gccgtgtcgt
     7861 ttggaccgat cggcgtgccg gcgaggcgca gcgccacctt aacctccgtg gttgcatcca
     7921 ccgcagcgcg atcccagggc aggatggcat cgagacgggc gcagaacgcg tcaaccagct
     7981 gcacgtggcg tggcgatgcc tttggaccgg tggcgccgaa gcgcatctcg gagtaggtga
     8041 tggccgagac cacgatacgg tgaccgcgca gcaccgcctg ctccagattc ttaagcactg
     8101 cttcaggctg ctcgcgcatg atgaacgagc agatgttcgt gtcgagcata tagatcttgt
     8161 tcacaggtta aatcgtcctt cgtcgcagac aacgtcctcg cgttccgtca taaagcccgg
     8221 atcggccttt tctagctcca ggaatgagct ccagctcggg cggaggggac gcagaatgat
     8281 tctgtcccct tcccgtacaa tctccagctc actcaccccc tcaaaatcca gatcgcgggg
     8341 cagacggatc gcgcggttat tgccattttt gaaaatagat acggttctca tgtcttcctc
     8401 ctggcaaggc ggcttttata tatgtttagt atatgcagat gtgatacata tgtatatgtt
     8461 ataaatatgc agatgtgtga gatatgcgta tgcctgtcat atgcagatgc caaacatatg
     8521 gatatgcatg gtatctgcat gtttctctct tttttcaccg ggatccgaaa aatat
//
LOCUS       DAEVZA010000174         8442 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEVZA010000174 DAEVZA010000000
VERSION     DAEVZA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.174"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..575)
                     /locus_tag="LAF92_004492"
     CDS             complement(<1..575)
                     /locus_tag="LAF92_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBI9793393.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILEL"
     gene            complement(646..2760)
                     /gene="fusA"
                     /locus_tag="LAF92_004493"
     CDS             complement(646..2760)
                     /gene="fusA"
                     /locus_tag="LAF92_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBI9793394.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2857..3327)
                     /gene="rpsG"
                     /locus_tag="LAF92_004494"
     CDS             complement(2857..3327)
                     /gene="rpsG"
                     /locus_tag="LAF92_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBI9793395.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3424..3798)
                     /gene="rpsL"
                     /locus_tag="LAF92_004495"
     CDS             complement(3424..3798)
                     /gene="rpsL"
                     /locus_tag="LAF92_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBI9793396.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3924..4211)
                     /gene="tusB"
                     /locus_tag="LAF92_004496"
     CDS             complement(3924..4211)
                     /gene="tusB"
                     /locus_tag="LAF92_004496"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBI9793397.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4219..4578)
                     /gene="tusC"
                     /locus_tag="LAF92_004497"
     CDS             complement(4219..4578)
                     /gene="tusC"
                     /locus_tag="LAF92_004497"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBI9793398.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4578..4964)
                     /gene="tusD"
                     /locus_tag="LAF92_004498"
     CDS             complement(4578..4964)
                     /gene="tusD"
                     /locus_tag="LAF92_004498"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBI9793399.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4964..5686)
                     /locus_tag="LAF92_004499"
     CDS             complement(4964..5686)
                     /locus_tag="LAF92_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI9793400.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5853..6665)
                     /gene="fkpA"
                     /locus_tag="LAF92_004500"
     CDS             complement(5853..6665)
                     /gene="fkpA"
                     /locus_tag="LAF92_004500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI9793401.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6886..7104
                     /locus_tag="LAF92_004501"
     CDS             6886..7104
                     /locus_tag="LAF92_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBI9793402.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7153..7743)
                     /gene="slyD"
                     /locus_tag="LAF92_004502"
     CDS             complement(7153..7743)
                     /gene="slyD"
                     /locus_tag="LAF92_004502"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI9793403.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7838..8038)
                     /locus_tag="LAF92_004503"
     CDS             complement(7838..8038)
                     /locus_tag="LAF92_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBI9793404.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8048..>8442)
                     /locus_tag="LAF92_004504"
     CDS             complement(8048..>8442)
                     /locus_tag="LAF92_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="involved in potassium efflux; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBI9793405.1"
                     /translation="CNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELLQAGVTQF
                     SRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADTVQISRAR
                     EARRELEEIFQREMQQERRQLDGWDEFE"
BASE COUNT         2030 a         2234 c         2029 g         2149 t
ORIGIN      
        1 agttccagga ttttcgcttc ccactctgcg tcgccttcca gcgctttcag agcagaacca
       61 cgaacgatcg gagtgtcgtc gcccgggaag tcgtactgag acagaagttc acgaacttcc
      121 atttcaacca gttccagcag ctcttcgtca tcaaccatgt cgcatttgtt caggaacacg
      181 atgatgtacg gaacgcctac ctgacgaccc agcaggatgt gctcacgagt ctgcggcatc
      241 gggccgtcag tcgcagcaac taccaggatc gcgccgtcca tctgagcagc accggtgatc
      301 atgtttttaa catagtcggc gtgccccggg cagtctacgt gtgcgtagtg acgggtcggg
      361 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
      421 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      481 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      541 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      601 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      661 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      721 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      781 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      841 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      901 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      961 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
     1021 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
     1081 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
     1141 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
     1201 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
     1261 atcggtaact ttctggcgga tagtttcgcg gtaagcaacc tgcggtttac ctacgttcgc
     1321 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1381 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1441 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1501 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1561 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1621 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1681 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1741 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1801 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1861 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1921 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1981 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     2041 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     2101 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     2161 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     2221 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     2281 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     2341 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2401 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2461 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2521 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2581 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2641 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2701 gtcgatgtgc gcactgatac cgatattacg gtagcgtgcg atgggtgttg tacgagccat
     2761 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2821 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2881 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2941 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     3001 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     3061 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     3121 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     3181 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     3241 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     3301 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3361 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3421 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3481 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3541 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3601 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3661 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3721 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3781 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3841 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaaggtgtca
     3901 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3961 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     4021 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     4081 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtct
     4141 ccttcactga gcagacgcag cagcgcagca aaatccgtca gccagggtga acgatgtaac
     4201 gtgtgcagca taaacgcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     4261 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggtgtctgcg gatctaaccc
     4321 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4381 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctgacagcag
     4441 ctgaaaaacg ccatcagcaa taaagaagac ggccagatcg tcagttaatg cggaagttgc
     4501 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggtgcag tagaaaaaac
     4561 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4621 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaagc cagccccagc
     4681 cttcctgctt cagtttcatc gacgataccg cggcgtaacg ctgccgctac gcagatattc
     4741 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtca
     4801 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgatagaa aaagacgctg
     4861 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4921 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4981 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     5041 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     5101 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca ttcacttctt
     5161 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     5221 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     5281 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     5341 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5401 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5461 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5521 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5581 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5641 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5701 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5761 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5821 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5881 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5941 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     6001 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     6061 caacccggga taacaccgtc cagacggaaa gaaagcggtt caccacgggt gtaagagttg
     6121 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     6181 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     6241 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     6301 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6361 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6421 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6481 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6541 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6601 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6661 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6721 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6781 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6841 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6901 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6961 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     7021 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     7081 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     7141 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     7201 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     7261 atcgtggtga tgatcgtgcg cgccgtgaac gtggccatga gccagttctt cttcggtcgc
     7321 ttcgcgaatc gccacaactt caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7381 accatcaacc acgacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7441 gtcagtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7501 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7561 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7621 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7681 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7741 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7801 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7861 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7921 gtccgcactt aacacattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7981 cctgcgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     8041 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     8101 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacggtat
     8161 cagcatgcat cgggattaac tcccgcagca ttcgcatatc caggcggcga aaatgcagtt
     8221 gcgcccgctg tgcctgatgc ggatgcatgc caagcgtgac cagcgtcttg cgccccagct
     8281 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     8341 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8401 atatttccac cagcttcatg gtgtcttccg gctcgttaca gg
//
LOCUS       DAEVZA010000175         8429 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEVZA010000175 DAEVZA010000000
VERSION     DAEVZA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.175"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1793)
                     /gene="opgB"
                     /locus_tag="LAF92_004505"
     CDS             complement(<1..1793)
                     /gene="opgB"
                     /locus_tag="LAF92_004505"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBI9793406.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTA"
     gene            complement(2047..2541)
                     /locus_tag="LAF92_004506"
     CDS             complement(2047..2541)
                     /locus_tag="LAF92_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBI9793407.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            complement(2590..3327)
                     /gene="dnaC"
                     /locus_tag="LAF92_004507"
     CDS             complement(2590..3327)
                     /gene="dnaC"
                     /locus_tag="LAF92_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBI9793408.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            complement(3330..3869)
                     /gene="dnaT"
                     /locus_tag="LAF92_004508"
     CDS             complement(3330..3869)
                     /gene="dnaT"
                     /locus_tag="LAF92_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBI9793409.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            complement(3977..4450)
                     /locus_tag="LAF92_004509"
     CDS             complement(3977..4450)
                     /locus_tag="LAF92_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBI9793410.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            complement(4441..5211)
                     /locus_tag="LAF92_004510"
     CDS             complement(4441..5211)
                     /locus_tag="LAF92_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBI9793411.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASMTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            5830..6555
                     /locus_tag="LAF92_004511"
     CDS             5830..6555
                     /locus_tag="LAF92_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI9793412.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            6513..7190
                     /gene="bglJ"
                     /locus_tag="LAF92_004512"
     CDS             6513..7190
                     /gene="bglJ"
                     /locus_tag="LAF92_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBI9793413.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(7228..8016)
                     /gene="fhuF"
                     /locus_tag="LAF92_004513"
     CDS             complement(7228..8016)
                     /gene="fhuF"
                     /locus_tag="LAF92_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBI9793414.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            8157..8393
                     /locus_tag="LAF92_004514"
     CDS             8157..8393
                     /locus_tag="LAF92_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBI9793415.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT         2138 a         2012 c         2124 g         2155 t
ORIGIN      
        1 aatgccgttt tgcccttcca cattgtttta tcaacatgct ggacaatttg ctgaccgcca
       61 agctgcccct gcgagacaca ccagtcggtg gagagtgcca gttccggagc ccaaagttgt
      121 gccatcttgt agcaacggtc aacccagacg aaattgtcgc gtggagcgaa atcggccagc
      181 tggaaacgca gcggtgctga gtattcactt tccggcagcg gttctacgcg cttgtctgaa
      241 acccgcaaca gcagcggcaa acggaaatgg ctacccgaga aggcaatcat gtttttctgc
      301 tggtcgatag tgaactcttt catctcttta gggaatttcc acaggcggat gatatccggc
      361 ttccacgcca atgttttctc tttgatattg aggaagattt ccgacatcga ctgcccggat
      421 aaactgctac gaccaagtcc gagatagtta tcgccaccga gaatgtccag caccgtcgcg
      481 ccgttatcca tcgtgttacg cttcactgcc agcgtctctt gctgcggctt gtcgccacga
      541 atgacaaaaa acaggttatt gcgatcctgc ttattaaggt atttccacgc cgtgttgttc
      601 atcgctaaat ggtcagaaga gacgacgatg acggtatctt taaaccacgg tgacgctttg
      661 attttgttga taaacgccgc gatattctcc tggctgcaac ttaccgcgct gaatgactgg
      721 ttcggcttac cgtcaaaatc atattttttg cggttacagg tacgcgagat aaaaccatcc
      781 gggtgatggg tatcgactgt cagggtaaac agtgagaatc gctgacctga gcgggaaagc
      841 tcttcaaact ttttccacgc ttcatcgaga acggtatcgt cgtagaatcc ccagtcgttg
      901 cgatagtgcg ggtcggccac cacgcttttc agctcttctg agccgtataa gtggtcgaag
      961 ccatgcgact tcaggaacac atctttaccg gcaaaacgca gattcgcgcc ctgcacgaaa
     1021 tagttctgat aacccgagtt tttcaggata tcgcccagac agatgttctg cgggaagaag
     1081 ctggagacag aggcggaggc gttgccttca aagggggcaa acagcggtat gccgcactga
     1141 gaagccacca tgcccgcaat cgtgtaatcc gttcctggca gctgctgcgt gtggctgaaa
     1201 tccaggcctt catttttcaa cgcgcccagt tcaggcgtga gatccgggaa agcttcgtta
     1261 tcaaaatagg tccgctcgag actttcgccg tagatataaa ccaggttgag tttcgggtca
     1321 gggatagttt tcgacggctc tttataataa gccgcaaagt ccgggtcgcc atcgcgtgac
     1381 tgggatttca ccagttccgt tatctgacga aatgctgggc tggcgtccac cgaacccagc
     1441 gccagtaaga gcgccagcag gctgtaacca aaatggtgcg gatgatggcg acgacggcgc
     1501 aggatccagc ccagcgcacc gaacaccgct gtcagcccca gcacaatgcc gatacccggc
     1561 aggatgtatt tgctgacgcc agcaccggtc aggctgttgg ttaaggtata gagaaccgcg
     1621 tcgttaatac catcgccagt aaaatagtcg ctggcaaaca gggtgatatt taaaacgaca
     1681 aatagcccca gcaccgttaa tgtggctgca aaccaccagg tgttacgtcc cgctttccat
     1741 gcgtaaatca gcacagaggc gagaaaaagg gcgaaagaga gtagttctga caacgggcga
     1801 tcctcactaa accgggccgt ttagccaacg tttaagcttt ttgagaagca caatgtaatg
     1861 gcgtcgccat attgctgcaa ttctagtgtc aaaaaaatgc gatgttgtta ggaattagtt
     1921 tataaacaga cttttttgat cgcggtcgct tcccgtgtaa cagggggaaa accgtccagg
     1981 agcagggaaa agggcaggag agagccgcgt cagccgggta acagctaaaa cgcggcgttt
     2041 cgcggttcag gaaatgaagt tcagcccctg ctttaacacc agatcgcagg ctttggtttt
     2101 caccttttct gccagcggag tcgttccgat gttatccaga ttgagttgct gaccatcttt
     2161 ggttttcagc aaaccctgaa tgccatccag atagttggtg tcttcttttt gctcttcact
     2221 gttcaggccc agcttttcca gcacctggtt cttgatgttt tcggcatcgg ttaccgacgc
     2281 cagcttttgc ttcgcgcagt attgcagaat gcctgcggcg ttgttcatgt tatctgcgct
     2341 taaggcctgg ttcccgctgc taagcaagtt agttaatgac gcgagcgacc aaccgccttc
     2401 ctgtgtcgtg ctgttttggt tgccaagttc gctggcggcg ctggagagcg catctttcca
     2461 ggacgcagca tgcaccccgg tggaaattaa tgcgctggcg gcaatggcac agcacagaag
     2521 atgtttaaca gttttcatca tcattactca aggtggaatt gtgtcgcagt ataccgccag
     2581 aaacggggat taatactctt tacctgttac ccggctacgg tagctatccc agttgaagat
     2641 cacccacaaa ctgttaccca ggcgcatacg gtccatcacg cgttcgccca gcaacttggt
     2701 catctcttcc atattactgt tggtcagcat cccggttggg cgtttggaag aagagcggcg
     2761 atcgacgatc tggttgatga tcactttttc gtatttcgat tcggtctgca cgccgatctc
     2821 atcgatcacc agcagatcaa cgttgctcag atcgttaagc agttgttctt cactggtacc
     2881 gctattcctg aaggtatctt tcatcgccga cataatatcg gccacggtga tgatcaatac
     2941 ggatttaccg cgtagcagca gctcgttgca gattgccgcc gccagatggt ttttgccggt
     3001 tcctggctta ccagaaaaga tgaagctggc gatgttgccg tcaaactctt cgacgtactg
     3061 gcgcgctttg cttaacgcat tcatctgccc ttcacactca acgcgatagt tctcaaagga
     3121 gcagttctga tgcagtggcc gaataccgga gcggttaaag gtgcgctgca ttttcatcgc
     3181 ccgattttca cgttcgagag cggcggagcg gatcgctcct tgttctttct gccaggccag
     3241 tagttcttca cccgttttga atgcaggttt gatatgggca ggcatcattt tttgcaggcg
     3301 ttgcatcagg tcgccaacgt ttttcatcgt taccctctga atcctggtgg aatttggctg
     3361 tcaggttcac tgaccgtatt cacatctcgt ttcggcagtc cgccgttgct ggcacgaccg
     3421 atttgcaggc tgcgcgccag tttttgttgc cactgtacat ggtgaaagac tttaccttcc
     3481 gcctgccagt aggcaatgaa tgaggtcagt tcttcggtgg tcaccggctc tcttagtgcc
     3541 acgccccata gcgccgccag gcggataaag tcggcatcgg gttgccagtc cggatacatg
     3601 gcgaatttcc ccatgggtac agcaacggga gcagcttgcg gttcctgata gagttgatcg
     3661 tccagagcga catcgcttcc cggacgcgcc agcttttctt ccagcgccag cagttcagcc
     3721 aggcgtgcag gcgtgacggc ataaaacgcc ggggcattgt tagcaaatac ggcaaccaca
     3781 ccgccttcag cttttgccag aacggtttgg tgatcgtgta ccagggcgtc aataccaacg
     3841 acgtccgggg tcaaaactct ggaagacata ctgtttctca aatatggaac gatgaacgag
     3901 aagggagata acttttatag taacacacgg aggagaggag gctgtctctc atccgtgtgc
     3961 tttaagcgag gcaattttat acgcgagggc gcttgcggta caaccataat ccaggaatgg
     4021 aaagaccgat ggataatgca ccaacaatcg atgaagctgt aagaaagttg gttaacaggg
     4081 taatcatcaa cggttcgctg taacctaact ggctgatttt taccgccgaa atcattgcgg
     4141 tataagccga tatgcccggg aacataggga taacggccgc cacggtaaac actttcggat
     4201 gcgccagata ccagcgcgac cattgaatac caatggtacc gaccagcata gaagccataa
     4261 aggttgacca ctcaatattc aacccgctgg tcatcaagat cattcgggaa ccatgaccaa
     4321 tcgagccaag cagcgcacac cagcgtaacg cccgcacggg aacgttgaac accatcgcaa
     4381 agccgaccgc aggaatggcg gcgaggatca tatcctgcgc caacgctaac agaaattcga
     4441 tcacacccat ccgcgtagcc cccaaatcgt cagtgccatc actacgccga cgcaggtagc
     4501 cagtgtcagc agactggcga tcgcccagcg tgccagtccg gtattgatgt ggcctttaaa
     4561 catatcggcg acggcattaa tcaacggaaa gcccggcacc agcagcagaa cgctggcggc
     4621 catcgcaatg gtgggggtat tgctgaaagt ggggagttgc aaaagcaatc cggaaatggt
     4681 ggtggcggcg aaagcggtaa ggcaaaagtt gatctgtgga tgaagatgac gttgtgccag
     4741 cagctggcgg atatacatcg cggtcatact ggcaaagaag gtgatgacgg caccatccca
     4801 gccaccgtta ttgagtttac agaaacaggc gcaagaaagg ccaaccatta aggctaccag
     4861 ccatcttggg taacgtaatg gctgaatttg gctaaatcgt ttctctacgc ctttgtaatc
     4921 cagcagatga tgctccgcaa gaatcacaat gtgctggact tcagtcacca catgcatatt
     4981 aatgccgcga tcgtgatttt tacgtgtcga tgtcaggcat tgcccatctt taatagttgt
     5041 cagcactatg gcgttcgaag agatagaact ttcgacgctg tccattccca gcgcccgacc
     5101 cagtcgtgag gaaagctcat caaccaacgc gctttccgca ccatgttgca aaagaaataa
     5161 tccacactgg atacaaagcc gtgttacggc tcgctgttgc tcagtttgca taactcgccc
     5221 tttgtcatag acgtgctgcg tattgttcag ataatgagaa tccatttgta tcatgaagca
     5281 cggttgtttt gcggtaggtt aagatcaaaa ttgcgctgat taatcctggt tatcgggaga
     5341 agctcggtga taaaaaacga attatctctc gccgcaaaca aactatcaca tgaatattat
     5401 catcataatg aatttattgt ttggccttta cgaatcagga taatagataa ccgggcacgt
     5461 tttttaatgt taacaagtta aaactatcag atttcattga ataaatattg gttgtgtgat
     5521 ttttcattct gcgggaaggg atatcattta attataaatt aaatgaatgt gatttctgtt
     5581 tttcattaag tattcctaac aacttaatgt agatatttta aatgtctcca ggctatttca
     5641 ccaggttatc atttgatgct aaccttctga cacatgcagt ggagttgttg tgcagcagga
     5701 gtatgctgat atgaaagtga atgctaagga taatttattc gcttaatcta ttaatttgct
     5761 ggaaacattt aaggaatgaa tatcctgaat aaaccatcat aatttttaga cagtggatgt
     5821 ggaggaaata tgttgccagg atgctgcaaa aatggaattg ttatcagtaa aatacctgtt
     5881 atgcaagcag ggctaaaaga ggtcatgagg actcacttcc ctgaatatga aataatatcc
     5941 agcgcctctg cggaggacct taccttatta caattacgtc gttccggatt agtcattgct
     6001 gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta ttctttaatc
     6061 tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta ttcccaggca
     6121 gtagaattgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc cattgagaat
     6181 ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc catgctgacc
     6241 tctccggcaa tgaatgaaac tcatgatttt agctatcgct ccgtcattct cactctttca
     6301 gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat agcttcattg
     6361 cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg tcgactggca
     6421 attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc aagagaactt
     6481 aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt aagaagagaa
     6541 atgtcgcact catagaaaaa tgcgtcatga gcagtatcgg tattgagagt ttattcagaa
     6601 agtttgcggg taacccttat aagctccata cctataccag tcaggagtca tttcaggatg
     6661 ccatgtcgcg gatctcattt gcggcggtca ttttttcttt ttctgccatg agaagtgagc
     6721 gcagagaggg attatcttgc ctgactgaac tggcgattaa gtttccgcgt acccggcgtt
     6781 tagttattgc ggatgatgat attgaagctc ggctgattgg ttcattgtcg ccatcaccgc
     6841 tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa ctctttttgt
     6901 cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac attaaccaaa
     6961 gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt ggctactcaa
     7021 tgacacaaat tgccgagcag cttaaacgca atatcaaaac gatccgtgca cataaattta
     7081 atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc gcagatattc
     7141 tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa tccgcattat
     7201 gccgggttta cttccccggc agtgctttca tttcagcgta caatcgccac attgctgcac
     7261 atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccat cgcgcagtac
     7321 cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa aaaagagggc
     7381 atggcgcagc gattcaaccg tcgcctcgcc gagcagttgc ttcatctcag taagatacca
     7441 gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc cggtcgcttc
     7501 tagtgcctgc acaaccggaa ccagcgcctg gctgattaac ctttccattc gctgctgcgg
     7561 cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg cggcacggcc
     7621 ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgcct tttcctgcgt
     7681 cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg cccatagtga
     7741 gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat gatcggaata
     7801 gaccgcgagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg tcatggcgtt
     7861 aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat gttcggcgat
     7921 cgtcgcacgt acggcttgtg caagcccagc atcctgtggc tggagatgcg ttcgccagat
     7981 aacatcttca tagagcggtg cggaacgata ggccataatc gggatagtaa tctaaatgat
     8041 aatgattgct aatcctagcg ataggtttac ccgatagcaa gggatttctc tggcttgcaa
     8101 atgataaaaa ttatcatatg atattggtta tcattatcaa tgaaagagat gaaaccatgt
     8161 tgcaacgtac gctgggcagt ggctggggag tgttgctgcc aggattgctg attgcagggc
     8221 tgatgtatgc ggatttatca ccggatcagt ggcggattgt cattctgatg ggattagtat
     8281 tgacgtcggt gatgctgtat cacaaacagt tacggcatta cgttttgcta ccatcgtgcc
     8341 tggcgcttat tgctgccatc atgctgatga taatgaattt gaatcaggga tgaaaaatca
     8401 aggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DAEVZA010000176         8411 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEVZA010000176 DAEVZA010000000
VERSION     DAEVZA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.176"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            271..3456
                     /gene="rne"
                     /locus_tag="LAF92_004515"
     CDS             271..3456
                     /gene="rne"
                     /locus_tag="LAF92_004515"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBI9793416.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAASVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(3652..4605)
                     /gene="flgL"
                     /locus_tag="LAF92_004516"
     CDS             complement(3652..4605)
                     /gene="flgL"
                     /locus_tag="LAF92_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBI9793417.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(4617..6260)
                     /gene="flgK"
                     /locus_tag="LAF92_004517"
     CDS             complement(4617..6260)
                     /gene="flgK"
                     /locus_tag="LAF92_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBI9793418.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(6326..7267)
                     /gene="flgJ"
                     /locus_tag="LAF92_004518"
     CDS             complement(6326..7267)
                     /gene="flgJ"
                     /locus_tag="LAF92_004518"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBI9793419.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFQLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(7267..8364)
                     /gene="flgI"
                     /locus_tag="LAF92_004519"
     CDS             complement(7267..8364)
                     /gene="flgI"
                     /locus_tag="LAF92_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBI9793420.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
BASE COUNT         1952 a         2320 c         2167 g         1972 t
ORIGIN      
        1 attgatcttt caggcagtta gcgggctgcg ggttgcagtc cttaccggta gatggaaata
       61 tttctggaga gtaataccca gtctgtttct ttgataattg cgctgttttt ccgcatgaaa
      121 aacgggcaac cgacactctg cgcctctttg agctgacgat aaccgtgagg ttggcgacgc
      181 gactagacac gaggccatcg gttcacaccc ggaaaggcgt tactttgccc gcagcttagt
      241 cgtcaatgta agaataatga gtaagttacg atgaaaagaa tgttaatcaa cgcaactcag
      301 caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc tgtatgacct ggatatcgaa
      361 agtccagggc acgagcagaa aaaggcaaac atctacaaag gtaaaatcac ccgcattgaa
      421 ccgagtctgg aagctgcttt tgttgattac ggcgctgaac gtcacggttt cctcccacta
      481 aaagaaattg cccgcgaata tttccctgct aactacagtg ctcatggtcg tcccaacatt
      541 aaagatgtgt tgcgtgaagg tcaggaagtc attgttcaga tcgataaaga agagcgcggc
      601 aacaaaggcg cggcattaac cacctttatc agtctggcgg gtagctatct ggttctgatg
      661 ccgaacaacc cgcgcgcggg tggcatttct cgccgtatcg aaggcgacga ccgtaccgaa
      721 ttaaaagaag cactggcgag ccttgaactg ccggaaggca tggggcttat cgtgcgcacc
      781 gctggcgtcg gcaaatctgc tgaggcgctg caatgggatt taagcttccg tctgaaacac
      841 tgggaagcca tcaaaaaagc cgctgaaagc cgcccggctc cgttcctgat tcatcaggag
      901 agcaacgtaa tcgttcgcgc attccgcgat tacttacgtc aggacatcgg cgaaatcctt
      961 atcgataacc cgaaagtgct cgaactggca cgtcagcata tcgctgcatt aggtcgcccg
     1021 gatttcagca gcaaaatcaa actgtacacc ggcgagatcc cgctgttcag ccactaccag
     1081 atcgagtcgc agatcgaatc cgccttccag cgtgaagtcc gtctgccgtc cggtggttcc
     1141 attgttatcg acagcaccga agcgttaacc gccatcgaca tcaactccgc acgcgcgacc
     1201 cgcggcggcg atatcgaaga aactgcgttt aacaccaacc tcgaagctgc cgatgagatt
     1261 gctcgtcagc tgcgcctgcg tgacctcggc ggcctgattg ttatcgactt catcgacatg
     1321 acgcctgtac gccaccagcg tgcggtagaa aaccgtctgc gtgaagcggt gcgtcaggac
     1381 cgtgcgcgta ttcaaatcag ccatatttct cgctttggcc tgctggaaat gtcccgtcag
     1441 cgcctgagcc catcactggg tgaatccagt catcacgttt gtccgcgttg ttctggtact
     1501 ggcaccgtgc gtgacaacga atcgctgtcg ctctctattc tgcgtctgat cgaagaagaa
     1561 gcgctgaaag agaacaccca ggaagttcac gccattgttc ctgtgccaat cgcttcttac
     1621 ctgctgaatg aaaaacgttc tgcggtaaat gccattgaaa ctcgtcagga cggtgtgcgc
     1681 tgtgtaattg tgccaaacga tcagatggaa accccgcact accacgtgct gcgcgtgcgt
     1741 aaaggggaag aaaccccaac cttaagctac atgctgccga agctgcatga agaagcgatg
     1801 gcgctgccgt ctgaagaaga gttcgctgaa cgtaagcgtc cggaacaacc tgcgctggca
     1861 acctttgcca tgccggatgt gccgccagcg ccaacgccag ctgaacctgc cgcgcctgtt
     1921 gtagccccag caccgaaagc tgcaccggca acaccagcaa ctcctgcaca acctgggctg
     1981 ttgagccgct tcttcggcgc actgaaagcg ctgttcagcg gtagtgaaga aaccaaaccg
     2041 accgagcaac cagcaccgaa agcagaagcg aaaccggaac gtcaacagga tcgtcgcaag
     2101 cctcgtcaga acaaccgccg tgaccgtaat gagcgccgcg acacccgtag tgaacgtact
     2161 gaaggcagcg ataatcgcga agaaaaccgt cgtaatcgtc gccaggcaca gcagcagact
     2221 gccgagacgc gtgagagccg tcagcaagct gaggtaacgg aaaaagcacg taccaccgac
     2281 gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta acgatgataa acgtcaggcg
     2341 caacaagaag cgaaggcgct gaatgttgaa gagcaatctg ttcaggaaac cgaacaggaa
     2401 gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc agctcaatca gaaagtgcgt
     2461 tacgagcaaa gcgtagccga agaagcggta gtcgcaccgg tggttgaaga aactgccgct
     2521 gccgaaccaa ttgttcagga agctccagct ccacgcacag aactggtgaa agtcccgctg
     2581 ccagtcgtag cgcaaactgc accagaacag caagaagaga acaatgctga taaccgtgac
     2641 aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc acctgcgcgt gagtggtcag
     2701 cgtcgtcgtc gctatcgtga cgagcgttat ccaacccagt cgccaatgcc gttgaccgta
     2761 gcgtgcgcgt ctccggaact ggcctctggc aaagtctgga tccgctatcc aattgtacgt
     2821 ccgcaagatg tacaagttga agagcagcgc gaacaggaag aagtacaagt gcagccgatg
     2881 gtgactgagg tccctgtcgc cgccgctgtc gaacctgttg ttagcgcgcc agttgttgaa
     2941 gaaatggctg aagtcgtaga agcccccgta ccggttgccg aaccgcaacc ggaagtggtt
     3001 gaaacgacgc atcctgaagt gattgccgcc gcggtaactg aacagccgca ggtgattacc
     3061 gagtctgatg ttgccgtagc ccaggaagtc gcagaacacg cagaaccggt ggttgaaccg
     3121 caggaagaga cggcagatat tgaagaagtt gccgaaactg ctgaggttgt ggttgctgaa
     3181 cctgaagttg ttgctcaacc tgccgcgtca gtcgtcgctg aagtcgcagc agaagttgaa
     3241 acggtagccg cggttgaacc agagatcacc gttgagcata accacgctac cgcgccaatg
     3301 acgcgcgctc cggcaccgga atatgttccg gaggcaccgc gtcacagtga ctggcagcgc
     3361 cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc atacggcaac gcatcatgcc
     3421 tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat tagctcaaag taatcaagcc
     3481 ctggtaactg ccagggcttt tttatttcat ctttgaatca acatattaaa attcatcaaa
     3541 tggcatcctt gctaaccaac aatgcaaaat aggcaaaaaa atgcccgccc cggcgatccc
     3601 agagcaggca gacaaaacat acccagtttc atgatatgtt ttaaagcgag cttatttgtt
     3661 gagctggaag agcgacaatc cctgcatatc ggtaaatgct ttatacgatg cctgcaatgc
     3721 cgtttgctgc atgatgtaag atgaaatagt tgcattccag tccacatcaa ccagatcgct
     3781 catctgctgc gtttgcccta aagcgcgatc gctacctaat gaatccagcg actccagttc
     3841 gttcagctgc gtgcctaatt ccgcgcgaac tgtaaggaca ttattcagcg agtttttcag
     3901 tccacggttg gttttatcca atgccgcagc ggcgatttct ttatccgctt cgctatccgc
     3961 gaccggcgtt ttcagggctg cgatggcgct atccagcatg gcaaaaagat tggtttcaga
     4021 agcgctaccg tctggttccg ctaccgcgtt actggtaata ctgtcgaaaa ttttgtcgcc
     4081 cgtatgtcct atcaccatcg aacgcgaagc atcgacctgt tgcctaatac tttctgctcc
     4141 accgacgtat ttccctttct cttcgctaaa cggcgcagtc tctgttttat aaccggcaaa
     4201 aatgtagcgc ccgttaccgt cagtggtgtt cgccagattc agcaactggt cacgaagccc
     4261 ctggatatcc gtagccagcg aggcccggtc atcgtcactc aaagtgccat tgctggcgta
     4321 gacaattttt tcctgagcat tctggatagc agtggtgacc tggctaagta cactctcttc
     4381 cagtgacact ttttgagtgg cgaaagtacg cgccagcgtg tactggctgt tttgcgcctg
     4441 tgcctgggag agaacaaccg cttgtgatgc tgcaatggga tcgtcagaag ggttaacgac
     4501 tcgcttacct gtcgacatct gctcgccgta cttcatccat tccgcctgag aattggtgat
     4561 gccacgcatg ttttgctggt acatcatctg ggtactgaaa cgcatcttat ctccccttag
     4621 cgaatgttaa tcagcgcatc aaaaatcgcg tttgccgtct gcagaacctg cgcattcgcc
     4681 aggtaatact gctgaaaacg ttgcagattt ccgtactcct catcgagatt gacaccggaa
     4741 atcgactgct gctgattgga aagctgcgtc accacattac cttgcgtggc gctactggtt
     4801 ttcaacgtcg cggttttatt accgatatca ctcactaacg aggcataagc gtcgttaaag
     4861 gatttcgcac cgcccaccgt tttactgttg ctttgcagat ccagcagggc ctgaccgttg
     4921 cggttatcgc tatcacccgc atcttcttcg ctcgccatcg ctattttcgc ttcgtcggtg
     4981 attaatacat ccatgttgac gatagcgtca cttactggtt tcagcgtgaa gctgtcgtta
     5041 acagcaggcg ttcctgtaaa cgtcaactcc agaccatcaa atgccacttt accgttggca
     5101 tccggcgtca ccgtaaaagt ggtattactg gcaaggcggg tgacctgcca ctgattatta
     5161 tcgaacgaga ttttgtaatc agtcgccagt accgcggagg catcagttac cgtggcaccg
     5221 atcgcaacgt cacctttgtt tttcgtgttt tgcagaaccg cgggcttacc gatagcaaag
     5281 aaatcttcac cggcatcgcc gttagcatca aatccggctt tgtgttggct gttgaaagcc
     5341 tcggcaaatg ccagcgccag ttgtccaagc gtattacgcg tctggtccag atcctgagaa
     5401 cggaatgtca gaatgccgcc cagcgacccg gtattcagta atttctccgg gatctcaata
     5461 ttgcctgccg tcccatcaac gtaagcgaca gtcgtacgag aagggtcggc gctggaagga
     5521 actgccgcca gttgccgcgc cgtacttccc tgaaccagtg agtaaccatt ggccatcgtg
     5581 atgttataag taccgccatc ctgaacgctg acttctacgc caacaatctg gtttaattcg
     5641 ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg acgcccctgc ccccacgcct
     5701 gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt tagcgtaatt gttgatctga
     5761 tcaacgctgg cacctatcgc gatattgacc tgtttgtcct ggtcgcgcag atattgatcg
     5821 gtggttttaa actgattcac caacccttct gatttcccaa tcagcgcctg gcgcgctgcc
     5881 gggtcttccg cgttactcac cagcgtttgc aggctggtga agaaatcctg catctgtgtt
     5941 gccagcgaag aggtactggt ggagagcata ttgtcgattt tcgacatctg ctcatagcgg
     6001 gcagtcaggc cgctactttg cgtctgcgca gcacgtaact ggttggtaat aaacgcatca
     6061 tactcacgct gcacaccaga aacgtagacg ccattgccaa cccagccgcc agcgcccaac
     6121 gtgctattgg cctgcgccat aatagtggtt tggcgggtat atccggcaac gttatagctg
     6181 gagatattat tacttgccgt atttaacgcc gcctgggccg cgttcagtcc gctcatggcg
     6241 ttattaatca agctggacat ggaggttcct tataagcctt caattacaga gtattatcgg
     6301 cagcgacccg ccggacttga gttattcaga acagattatc aatgttcata ctgtaggttt
     6361 tgctcacctt gtcgcttatc gatttcatct gctgaatcat gttggtgagt ttgcgggcat
     6421 agtgaggatc ggtggcatag cccgcgtcct gtagggcctg cgccccctgt tccgcactcg
     6481 cggcggtcgt cacggcggcg tagcgcgggt tacgcgttaa cagcccaacg taatccgaca
     6541 aggcttccag atacgagctg tagacgcgaa acttcgcttt tactttcttc gcttcgccgt
     6601 tttcatattc agtcgtggtg atttcagtaa ctggcccttt ccagttgcca gaggctttga
     6661 caccaaacag gttatagctc ggctcgccgt tttcgcggcg gatttgccgt tgcccccaac
     6721 cagattccag tgccgcctga gcgaggatca aatgatgtgg cacaccgctt tgctggcttg
     6781 ccagttgggc gggcagcgag agttgcgcga ggaatgcttt actgtcaccc ggcagcgaat
     6841 catcgtagtt acgtggcacg gccttttgca ccagctgcga aagcgcctga ttttgataac
     6901 gcaccacagt ttcgagctgg aatttcatcg gtgctgctgg cgtggactcc tctggcaatg
     6961 gttgttctgg cgtcatctgt ttaaccatca tctctgcaag ccccagacct ttgcccgccg
     7021 tcatctgttg ggcaatttgc tggtcataca tactggtata cagacgagtg tgctcgctgc
     7081 tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca
     7141 tcccttccac ctggcgggcc accggacgga tatttgccgc cggatcttcg cccgctttcg
     7201 cctttagttc gttgagtgat tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc
     7261 tgatcatcag atgatttcca gttttgcccg cagacatccc gcactttgca ttgattgcaa
     7321 aatagacatc agatccatcg gcgtagcgcc cagcgcattg agcgcgcgca ccacgttatt
     7381 gaggctggcg ctggaacgta cgctttgcag cgaaccgccg ctctggcgta aatcgatctg
     7441 cgtttgtgga gtaaccacag tctgtccacc accaaacggt gtatctggct ggctgacatt
     7501 ggcctgacga ttgactgtta ctgagagatt tccctgcgct accgcgcagc tgtcgagggt
     7561 cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta atcactactt tagcgtcctg
     7621 cggggtgaca ttaacctgca tattctggat atctgcaagg aaacggacct gggaactgtt
     7681 gccacttggt acgcgcacct gaatagtccg cgcatctaac gcggtggcgc tgccatatcc
     7741 acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg ctgaaatctt cgtcgttaag
     7801 ttgcaaatta agggtattcc cgacgccaaa ctggctgggc aattcacgtt caataaccgc
     7861 accattggtg atccgtccac cgttcagttg gttcacctga acactgctac cgccagcgga
     7921 ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc gcatacacct gactgtcaac
     7981 gcccttaagc ggcgtcatca acaacgtacc tccacgcaag cttttggcat ttcccatgga
     8041 agaaaccacc acatcgatgg tttgcccctg acgtccaaac ggaggaagtg acgctgtcac
     8101 cattaccgca gcgacgtttt ttagctgcat attggtgccc gtcggaacgg taattcccag
     8161 ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg tctggtcacc
     8221 ggtgccatcc agccccacca ccagaccata gccaatcagt gagttttgcc ttaccccctg
     8281 aacactggtg agatcgcgaa tacgctcagc ctgagccgcc gtcgtaacca gtagaagaat
     8341 taatgcagag agaaatttaa tcaccacagc ctcacttaca ttggcgacag gttaaggaag
     8401 aaacgctgca a
//
LOCUS       DAEVZA010000177         8358 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEVZA010000177 DAEVZA010000000
VERSION     DAEVZA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.177"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..66
                     /locus_tag="LAF92_004520"
     CDS             <1..66
                     /locus_tag="LAF92_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI9793421.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
     gene            175..537
                     /locus_tag="LAF92_004521"
     CDS             175..537
                     /locus_tag="LAF92_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBI9793422.1"
                     /translation="MRMVELASQPGACVAQIARENGVNDNVIFKWLRLWQNEGRISRR
                     IPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTTVSASSCKVEFRHGNMTLEN
                     PSPELLTVLIRELTGRGR"
     gene            534..884
                     /gene="tnpB"
                     /locus_tag="LAF92_004522"
     CDS             534..884
                     /gene="tnpB"
                     /locus_tag="LAF92_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI9793423.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPLSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPVVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            915..1136
                     /locus_tag="LAF92_004523"
     CDS             915..1136
                     /locus_tag="LAF92_004523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793424.1"
                     /translation="MSQKYLIRIAELESQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQARCRKNPGWAKRSAMY"
     gene            1088..1429
                     /locus_tag="LAF92_004524"
                     /pseudo
     CDS             1088..1429
                     /locus_tag="LAF92_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1493..1972)
                     /locus_tag="LAF92_004525"
     CDS             complement(1493..1972)
                     /locus_tag="LAF92_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBI9793425.1"
                     /translation="MMDYEDFSPKEQLQLTVCQRLIAEKSYFSQEELRRDLQERGFET
                     ISQSTVSRLLNLLGVIKIRNAKGLKVYSLNPQLRPAPDAARTVSEMVVSVEHNREFIL
                     IHTVAGYGRAVARVLDYHQLPEILGVVAGSSIVWVAPRMVKRVALVHKQINYLLRTY"
     gene            complement(2053..3456)
                     /locus_tag="LAF92_004526"
     CDS             complement(2053..3456)
                     /locus_tag="LAF92_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBI9793426.1"
                     /translation="MGKFKFPSAYTILFFLIAIVAVLTWIIPAGQYHMAMNEALGKEV
                     PVAGTYAHVEANPQGLISVLMAPIAGLYDPDSGQARAIDVALFILIIGGFLGIVTKTG
                     AIDAGIERVTTRLRGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMLAARFD
                     PVVAASTVLLGAGIGTLGSTINPFATVIAANAAGIPFTNGITLRVVVLVIGWIICVTW
                     VMRYARKVRKEPSLSIIADKQEENLAHFLGNKSEQALEFTPVRKIILVIFALTFAVMI
                     YGVAVLGWWMAEISTVFLASAIIIGLIARMSEEELTSTFINGARDLLGVALIIGIARG
                     IVVIMDKGMITHTILHYAEGMVTGLSTVAFINVMYWLEVVLSFLVPSSSGLAVLTMPI
                     MAPLADFANVNRDLVVTAYQSASGIVNLITPTSAVVMGGLAIAHVPYVRYLKWVAPLL
                     GILTVVIMVALSLGALL"
     gene            complement(3504..4508)
                     /gene="argF"
                     /locus_tag="LAF92_004527"
     CDS             complement(3504..4508)
                     /gene="argF"
                     /locus_tag="LAF92_004527"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBI9793427.1"
                     /translation="MTINLKKRNFLKLLDYTPAEIQYLIDLAIKLKAAKKAGREKQTL
                     VGKNIALIFEKTSTRTRCAFEVAAFDQGAQVTYLGPGGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQAIVEELGKYAGVPVWNGLTDEFHPTQILADLMTMLEHSPGKKLSELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDVVLVEQCRSIAEETGARITLTD
                     DVEEGVWGTDFLYTDVWVSMGEPKEAWTERVSLMKPYQINADVMNATGNPNVKFMHCL
                     PAFHNEHTKVGREIEMAYGLKGLEVTEEVFESPNSIVFDEAENRMHTIKAVMVATLGD
                     "
     gene            complement(4593..5504)
                     /gene="arcC"
                     /locus_tag="LAF92_004528"
     CDS             complement(4593..5504)
                     /gene="arcC"
                     /locus_tag="LAF92_004528"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006810310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBI9793428.1"
                     /translation="MERKPTLVVALGGNALLKRGEPLEAEIQRQNIELAARTIAGLTV
                     NWRVVLVHGNGPQIGLLALQNSAYDKVTPYPLDVLGAESQGMIGYMLQQALKNSLPQR
                     EVSVLLTQVEVDATDPAFSNPTKYIGPVYNEDQAKTLAAEKGWGFKADGSYFRRVVPS
                     PQPKRIVESDAITALIQRDHLVICNGGGGVPVVEKANGYRGIEAVIDKDLSAALLAYQ
                     IGADALLILTDADAVYLDWGKPTQRPLAQVTPELLRGMQFDTGSMGPKVAACCKFVEA
                     CNGIAGIGALVDGAEILAGNKGTLIRN"
     gene            complement(5515..6735)
                     /gene="arcA"
                     /locus_tag="LAF92_004529"
     CDS             complement(5515..6735)
                     /gene="arcA"
                     /locus_tag="LAF92_004529"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBI9793429.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRDQGVEVLLLTDLLTQTLDIKEAKTWLLETQISDYRLGPTFAGD
                     VRSWLADMPHRELARRLSGGLTYGEIPAAINNMVVDTHTSNDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPAFADGDFIKYFGDENIYYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGVEFLANSLFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDVDTFSVYPEVVRKDAQCWTLTSNGRDGLQRTQETDLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTIRPGVVIGYERNVWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLERDGI"
     gene            7628..8110
                     /locus_tag="LAF92_004530"
                     /pseudo
     CDS             7628..8110
                     /locus_tag="LAF92_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609301.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(8056..8358)
                     /locus_tag="LAF92_004531"
                     /pseudo
     CDS             complement(8056..8358)
                     /locus_tag="LAF92_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="RepA; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
BASE COUNT         2065 a         2119 c         2173 g         2001 t
ORIGIN      
        1 tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat
       61 caataagttg gagtcattac ccttagacgc ccatctgaca cagacggaca tctaagtatg
      121 gaattacagg actgacgaaa agaacctcgt aaaaagtatt cgaatgaatt caaaattcgt
      181 atggttgaac tggcatcaca acccggtgct tgtgtggccc agattgcacg agaaaatggc
      241 gtcaatgata atgttatttt caaatggctc agactctggc aaaacgaagg gcgtatatca
      301 cggcgtattc cggtgacaac cacttctgac gcaggcgttg aattattacc tgtagagata
      361 acgcctgatg agccgaaaga accgatggct gctcttactc cgtccttatc cactcagact
      421 actgtcagtg ccagctcctg caaggtggag ttccgtcacg gtaacatgac gctggaaaat
      481 ccttcaccag agctgctcac tgtattgatt cgtgaactga ccgggagggg aagatgatct
      541 cactcccatc aggtacccgt atctggctcg ttgccggcgt taccgatatg cgtaaatcct
      601 tcaacggact gggagaacag gtacaacatg tgctgaatga taatcccctc tccggtcacc
      661 tgtttatctt ccgtggccga cggggtgaca ccgttaaaat tctttgggct gatgctgatg
      721 gtctgtgcct gttcaccaaa cgcctggagg aaggccagtt tatctggcct gtggtacgtg
      781 acggcaaggt atccattacc cgctctcaac tggcaatgct cctcgataag ctggactggc
      841 gtcagccaaa aacatcccgc cttaacgccc tgacaatgtt gtaaaaaacg cctggccgca
      901 ttataaaaac ggccatgagt cagaaatacc tcattcgcat tgcagaactg gaaagccagc
      961 tccgtcagaa agaccagcaa ctgagtctgg ttgaagagac ggaggccttc ctgcgctctg
     1021 cactggcacg tgccgaagaa aagatcgaag aagatgaacg ggaaatagaa catctgcggg
     1081 ctcaggcacg ctgtcgaaaa aatccaggct gggcgaagcg ttcagctatg tactgaatca
     1141 gtgggacgcc ctctgttatt acagtgatga cggtctggcg gaggcggaca ataacacagc
     1201 ggaaagagcg cttcgtgcat tctgtctcgg aaagaaaaat tacgtgttct tcggtagcga
     1261 tcacggcggc gagtgtggtg cactgctgta cgggctgatc ggcacctgcc gtctgaacgg
     1321 tatcgatccg gaagcgtatc tgcgccatat tctgagcgta ctgccggaat ggccctccaa
     1381 ccgtgttgac gaactcctgc catggaacgt agttctcacc aataaataag cgtcaatacg
     1441 gtgctccgtt gacgcttacg tattagtcaa tacaagcggg catttgtgaa tattaatacg
     1501 ttcttagtaa ataattaatt tgcttatgca ccagagcgac acgcttcacc atccgaggag
     1561 cgacccagac gatactgctt ccggccacca cgcctaaaat ttctggtaac tggtgatagt
     1621 caaggacacg tgccactgcg cggccatatc cagcaacggt atggataagg ataaattctc
     1681 tattgtgctc aacgctcacc accatttcgg acacagtgcg cgcggcatca ggagccggac
     1741 gcaactgtgg attcagcgaa tagactttta gccctttggc atttcgaatt tttatgacac
     1801 ctaacaaatt gagcagacga gaaacggtgg actggctgat tgtctcaaaa ccacgctcct
     1861 gtaagtcgcg gcgaagctcc tcttgggaaa aatagctctt ttctgcaatc agacgttggc
     1921 agaccgttag ctgtagttgc tctttgggag agaaatcttc gtaatccatc ataatcctat
     1981 attcagttct cgaaataacc ggtgacacca caccattccg tagaccggat aggggcagcg
     2041 ccatccggca aattacaaca atgcccccag acttaatgcc accataatta ccactgttaa
     2101 tatcccaagc agcggcgcaa cccatttcag atatcgcacg taaggcacgt gggcgatagc
     2161 cagtccgccc atcacaacgg cagaggtggg agtgatcagg ttaacgatac cggacgcaga
     2221 ctggtaagcc gtaactacca ggtcgcggtt gacgttagcg aagtcggcaa gaggtgccat
     2281 gatcggcatc gtcagaacgg ccagaccgga cgaagaaggc acaagaaacg acagcaccac
     2341 ttccagccaa tacatcacgt tgatgaatgc tactgtcgat aatccagtaa ccattccctc
     2401 ggcgtagtgc aaaatagtat gggtaatcat acctttatcc atgatcacta cgataccgcg
     2461 cgcgataccg ataatcagtg caacgcccag caaatctcgc gcgccgttga taaaggtaga
     2521 ggtcagttcc tcttcgctca tgcgggcgat cagaccgatg ataattgcgc tggccagaaa
     2581 taccgttgag atctccgcca tccaccaacc cagcaccgcc acgccgtaga tcatgaccgc
     2641 gaaggtaagg gcgaaaatca ccagaataat tttgcgtacc ggggtgaact ccagagcctg
     2701 ttcgctttta ttgccgagga agtgggcgag gttctcctct tgtttatccg caataatgga
     2761 gagagacggc tccttgcgaa ctttccgggc atagcgcatc acccatgtca cgcagattat
     2821 ccagccgatg acaagcacca ccacacgcaa ggtgataccg ttggtgaagg ggatcccggc
     2881 tgcattggcg gcgatcaccg tcgcgaaagg gttaatggtg gagccgagcg tgccgatccc
     2941 ggcgccgagc agcacggtgg aggcggctac cacagggtcg aagcgtgctg ccaacatcac
     3001 cggcaccagc agggtataaa acggcaggga ttcttcggcc atgccgtaaa ttgtaccgcc
     3061 agcagcaaac agcgccataa ggatcgggat catccactct tcgcgaccac gtagtcgggt
     3121 ggtgacgcgc tcgattccgg cgtcaatggc cccggttttg gtgacgatcc cgaggaatcc
     3181 tccgatgatc agaataaaca gcgcaacgtc tatcgcccta gcctgaccgg agtctggatc
     3241 atacaacccg gcaattgggg ccatcagcac tgaaatcagt ccctgcggat tggcctctac
     3301 gtgtgcatag gtgccggcaa caggaacctc cttgccgaga gcttcgttca ttgccatgtg
     3361 atactgcccg gctggaatga tccacgtcag gacggcaacg atggcaatca gaaaaaagag
     3421 aatggtatat gcggagggaa atttgaactt acccatgatg ttctcctgag acccggtgcc
     3481 ccctacgacg caccgggtgg tgattagtcg ccgagtgtcg ccaccatgac cgctttaatg
     3541 gtatgcatgc ggttttctgc ttcatcaaag acgatagagt taggggattc gaagacctct
     3601 tccgtcacct ccagtccctt caggccgtat gccatctcaa tttctcggcc cactttggtg
     3661 tgctcattgt ggaaggctgg caggcagtgc atgaacttga cgttcgggtt gccggtggcg
     3721 ttcatcacgt cagcgttgat ttgataaggc ttcatcaggc tgacgcgttc ggtccacgcc
     3781 tctttcggtt cacccattga gacccaaaca tcggtgtaga ggaaatccgt tccccacacg
     3841 ccttcttcca cgtcatcggt cagggtgata cgtgcgcccg tctcttccgc gatggaacgg
     3901 cactgttcaa caagcaccac atccggccag aaggattttg gggctacaag acggatatcc
     3961 atccccattt tggcagcccc caccatcagg gagttaccca tgttgttgcg tgcgtcgcca
     4021 aggtaggcaa agctcagttc cgacagtttt ttgcccgggg aatgttccag catggtcatc
     4081 aaatctgcga ggatttgggt tgggtgaaat tcgtcggtca gaccgttcca caccggtacg
     4141 cccgcgtatt tgcccaactc ctcaacgatg gcctgaccgt aaccacggta ttcgatgccg
     4201 tcatacatac ggcccagcac acgggcggtg tctttcattg actctttatg gccgatttgc
     4261 gatccgcctg ggccgaggta ggtcacctgc gccccttggt cgaacgcagc cacttcgaaa
     4321 gcacagcggg tacgggtgga ggttttttca aaaatcaggg caatgttttt gccaaccaag
     4381 gtctgttttt ctcgtccggc ttttttggcc gctttcagtt tgatcgcgag atcgatcagg
     4441 tactggatct ctgccggggt gtagtccagc agtttaagga agttgcgttt tttcaaattg
     4501 atggtcatta ggaaatcctt tttaaatttt aactgtctga tgccctcact ctgccatctc
     4561 ccctttgagg agagggttag gtgaaggggg attcagttac gaatcaatgt gcctttattg
     4621 cccgccaaaa tctcagcccc gtcgaccaga gcgccgatcc cggcaatacc gttgcaagct
     4681 tcaacaaact tgcagcaggc ggccactttc ggccccatcg atccggtgtc aaactgcatg
     4741 cctctgagca gttctggcgt cacctgcgct agcggacgct gggtcggttt gccccaatcg
     4801 aggtaaaccg cgtcggcatc agtgagaatc agtagtgcgt cggcccctat ctggtatgcc
     4861 agcagggcag cagagaggtc tttgtcgatc accgcctcaa ttccgcgata gccgttagcc
     4921 ttttccacaa ctggtacacc accgccgccg ttgcagataa caagatggtc gcgctggatc
     4981 agtgccgtaa tagcatcgct ctcgacaatg cgtttcggct gtggagatgg caccacgcga
     5041 cggaagtagc tgccgtcggc cttaaacccc caaccttttt ctgctgccag tgtttttgcc
     5101 tggtcttcgt tgtacaccgg tccgatatat ttggtcgggt tgctgaacgc cgggtcagta
     5161 gcgtccactt ccacctgagt aagcaggacg ctcacctcac gctgtggcag gctgtttttc
     5221 agcgcctgct ggagcatgta gccgatcatc ccctggcttt cggcgccaag aacgtccagt
     5281 ggataagggg tcactttgtc gtaggcgctg ttctgcagcg ccagcagccc gatctgtgga
     5341 ccgttgccgt gaaccaacac cacgcgccaa ttcaccgtga gcccggcgat ggtacgggcg
     5401 gccaactcaa tgttctggcg ctggatttct gcttccagtg gttcgccgcg cttcaataat
     5461 gcgttgccgc ccaacgccac aaccaaagtg ggttttcgtt ccatgatggc tcctttaaat
     5521 tccgtcgcgt tcaagcggac agctcatgca gcgtgcgccg ccgcggcctc gtcccaattc
     5581 gtcccccggg atgggcagca cggtgatgcc ggctttgtcg tatttctcgt tagtccagac
     5641 gttgcgctcg taaccaatca ccacaccggg gcggatggtc agaacgttat tggcgtcgtt
     5701 ccactgctca cgttcggctt caaaggcgtc gccgccggtg gtgatcaagc gtacctggtc
     5761 aataccgagt gctttctcga tggcgtgcaa caggtcggtt tcctgggtcc gttgtaggcc
     5821 atcgcgtccg ttcgaagtga gcgtccagca ctgggcgtct ttgcgcacca cttccgggta
     5881 aacggagaaa gtgtcaacgt cgatgtgggt catgacggtg tcaaggtgca tacaggagcg
     5941 gtgttttggc agctcaacgg cgatcactcg ctcggcctga cgatgtttga acaggctgtt
     6001 ggcgaggaac tccacgccct gcggtgttgt gcgttcagac atgccgatca ataccgcccc
     6061 acgaccaatc actaatacgt cgccaccttc caaagtggcg tggtcgtaat aaatattttc
     6121 gtcgccgaaa tacttaataa aatcgccgtc ggcaaatgcc gggtgccagc gatatattgc
     6181 ccggaggtta ttggtttcac gttgacgggc tggtttggcc atcgggttaa tagaaacacc
     6241 gttataaatc cagcaggagg tatcgcgggt aaataaatga ttcggtagcg gcttcataat
     6301 aaagtcatta gacgtgtggg tatccaccac catattgtta atggcagccg gaatttcacc
     6361 gtaagttaat ccgccgctta atcttcgcgc cagttcacgg tgcggcatgt ccgccagcca
     6421 gctgcgcaca tcgcccgcaa aggtaggtcc gaggcggtag tcagaaattt gcgtctccag
     6481 taaccaagtt ttcgcttctt taatatcaag ggtttgtgtc aggaggtctg ttaacagcag
     6541 gacttccacc ccctgatcgc gcagcgtatt tgcgaagatg tcatgctctt cacctgcccg
     6601 ttcaaccgag agtacatcat cgaaaagcag ttcctgacaa ttcgatggtg tcaaacgttt
     6661 cagacttaaa tttgggcggt gcagcataac actacgcaat tgaccaattt cagaaccgac
     6721 gtaatgtttt tccataatta ttcctttaat ttttttagaa taaaaaggta tgcgccatgt
     6781 tgtgtattta atttcatggc ggctaacttc aattgatttc gtggttcata ctaggcggta
     6841 aaataaacat tattgtgatg agattcacac aataatggat attaaggtat ttgttttaat
     6901 ttgtgttact tgcattaaat aatgattaat ttcataatgt cagctcatga atagatagtc
     6961 attatttggg tggaaatatc tctatttgtt attattttgt ggagtttgct gttttaaggt
     7021 gattatatat ttatatgatt ttaaaagtaa aaaaactgta gcgtgaataa ttatgaaata
     7081 attgcaattt aacttttttt ggcttaataa aaggctgtaa atagttatga aatcataaag
     7141 aaaaattatt aatgtgataa aatgtgatat gttctcaggt tttgtgattt tattttataa
     7201 ttctggtggt tgtgttggcg ttatcacacg ctcgaaatga agttatgcat aaccgctgca
     7261 taattacctg tgtatgatta acaatgattt aacacccttc ccgataaaca tgagctcgct
     7321 tagatgtcac aggaaaaggg ctagtatggc agcactggtt ttcgtatgaa agaaagataa
     7381 aacattgcct tatatctaag gcttcttgat tatcggtaac atccaccatc tccagccata
     7441 gctggcaggt ggtgacgttg gtgcagtact ggcgggagct tcggctccgg aactggcata
     7501 cctgctgaaa tcaacagaaa aagacccggc agtaaaggcc attgctcacg ccattctggg
     7561 cggtgcagta gcagcaatgc agagtaataa tgttgctgct ggtgcagcag gcgcagcaac
     7621 aggagagctg gcagccagag cgatcggaat gctgtatccg ggagtgaagc tgtctgacct
     7681 cagtgaagag cagaagcaga cgataagtac gctggcaacc gtgtcagcag ggctgaccgg
     7741 cggtctaacc ggaaacagca ccgcatcagc tgcagtggga gcacacagag cgggaagaat
     7801 gctgttgaga ataactacct gagcgtgtct gaaaagacag aacttgagat agcaaaacag
     7861 acgattaaaa acagtaaaga tccggcagag cgtgaaaagg cacagcagaa atatgatgca
     7921 ttgcttgaaa aagatattca gtgataagga ggttattgct gcctgtagta atggtaatgc
     7981 gggtagttca gcgtgtgcca gcgcaagact gaaagctatt gcgtcgaaag aagggtacga
     8041 agatggccct tataacgaga tatcggtcgg aatgttgcag ccaataagcg ggtcatattc
     8101 cgtctggtag ctaatcagcc ccagctctga caggaacgtc agggcacggg tggcacgggt
     8161 gatggagagt gttccagcag cagactccgt cgccagcccg cactcaatgg ccagcgtggt
     8221 gatggagcac tggacacggt tggccagcgg gtcatagtgg aaacacagcc cctgcagcag
     8281 cgcatcaata gcccgtcgac gcagcaccgg tggcatgcgc cgacgcagtc ccttcgaacg
     8341 ggcatgcgcc acatgaat
//
LOCUS       DAEVZA010000178         8177 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEVZA010000178 DAEVZA010000000
VERSION     DAEVZA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.178"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..215)
                     /locus_tag="LAF92_004532"
     CDS             complement(<1..215)
                     /locus_tag="LAF92_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBI9793430.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSF"
     gene            complement(292..596)
                     /locus_tag="LAF92_004533"
                     /pseudo
     CDS             complement(292..596)
                     /locus_tag="LAF92_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(600..1502)
                     /locus_tag="LAF92_004534"
     CDS             complement(600..1502)
                     /locus_tag="LAF92_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBI9793431.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTDAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(2369..3340)
                     /locus_tag="LAF92_004535"
     CDS             complement(2369..3340)
                     /locus_tag="LAF92_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HBI9793432.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDSLDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(3340..4506)
                     /gene="sopA"
                     /locus_tag="LAF92_004536"
     CDS             complement(3340..4506)
                     /gene="sopA"
                     /locus_tag="LAF92_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HBI9793433.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(5094..5849)
                     /gene="repE"
                     /locus_tag="LAF92_004537"
     CDS             complement(5094..5849)
                     /gene="repE"
                     /locus_tag="LAF92_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HBI9793434.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(6623..7429)
                     /locus_tag="LAF92_004538"
     CDS             complement(6623..7429)
                     /locus_tag="LAF92_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBI9793435.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(7430..7735)
                     /gene="ccdB"
                     /locus_tag="LAF92_004539"
     CDS             complement(7430..7735)
                     /gene="ccdB"
                     /locus_tag="LAF92_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HBI9793436.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(7737..7955)
                     /gene="ccdA"
                     /locus_tag="LAF92_004540"
     CDS             complement(7737..7955)
                     /gene="ccdA"
                     /locus_tag="LAF92_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HBI9793437.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
BASE COUNT         2177 a         2148 c         1903 g         1949 t
ORIGIN      
        1 tagaagctca ccatcagcgc gtcttttttg agtacgcggt acatttcatt acaggccggt
       61 tgcagccatt catcggtttt atcgccggcg atggtgcgcc cggaacggtc acgaaaaccg
      121 acgagatatg gcgggtcagt taatatgaaa tcaatggcgt tatcagggat acgggccata
      181 acatcgatgc agttaccgag gacaaaacgg gacataacaa cgctccttct cttacagagg
      241 gcaccgggag ggcccccctg tggtgcccgt tccccgctgc ctgtgggctg ttcaccgcgt
      301 ccggtaacgg gtcaggccga ccgtggaata tcgcagccgt cgtctttttg acggcgagaa
      361 tatgccgcga aagcggcttg aagggcgttc atcagaaaag ggcgacaggc tggcgtacac
      421 tgtcaccggt gccagccggg agcagtaaaa gcgcccgtcc cgtgcccgcc ggacgcggga
      481 cactgaccac aggcagcgtt cctgccgcgc cgcgtcagcc ggcgcagtaa aaagaggagc
      541 acggcgacat cctttgtcgg aaaagaaacg ggcggttgcc cgcccgtctc cgtcatactt
      601 catcccccgt aatgcgccac cttgtcgact atccactccg gcgcggtgac ttccggcagc
      661 tcatcatcat ctgtcgggga agaccattcc agttcccccc acagcacatc aacaagctct
      721 ccacgctcat atcgctgcca gccacagaaa ttgcagccct gttcggcgta ccagtgctcc
      781 agcgtgcagc cgaaacggcg gcttaattct gcaacaacgt tgctttccgg ctgacaccac
      841 ggcgtgtcga aatccacttg aatgaagttt tctccctgac ggctgatatt cacctcatac
      901 cccacaggcc acttcacgcc atactgttcc gtataccagt gatacgcaga aggaacaccg
      961 ttcagaacac caccgttaaa gccattcact tcaacatcca ggcgggtggg cagaaccgtc
     1021 agcaggtcaa acggcatggt tttttccggc agtacattgt cacacagacg gttccaccac
     1081 accgatacat cctcgttact ccagaagcca caccagtcac ctctttttgc ggtgaaatgc
     1141 acggtgatgg tttccctgac ctcatccggt aatccttccc agcgacgctg gccagtacca
     1201 ctctgcagcc agagttcatg cagcatcagg cagttttgct catccagcag tacaccgttc
     1261 tgcaggtgtg tcagccagcg ggtgaacgcg atattctccg gcgatacaac accacgtccg
     1321 gcatccgtca gtccggggca cggttcaaac tgcacatctt cagtggtctg cagaagtccg
     1381 gcacttccgg ccagaaacag ctgaatacct tcatttgtgg cgcggcgata aagcggtgtg
     1441 acggcaccgc tggccagtcg tttaatctca gcaatctgtg ccggttcacc agaaaaatgc
     1501 atacgattcg agcaccagtt tggcatggga attctcctct cacatgcggc ctccatcagg
     1561 ccgtgccgca cctgttaccg ggcgaccgca tacgaatgca ggtgcggccc cgaagccgca
     1621 gcgcgtggag gaggaacgac gaaacgtgac cgggtgagga ggccggcgac agcgacacac
     1681 ttgcatcgga tgcagcccgg ttaacgtgcc ggcacggcct gggtaaccag gtattttgtc
     1741 cacataaccg tgcgcaaaat gttgtggata agcaggacac agcagcaatc cacagcaggc
     1801 atacaaccgc acaccgaggt tactccgttc tacaggttac gacgacatgt caatacttgc
     1861 ccttgacagg cattgatgga atcgtagtct cacgctgata gtctgatcga caatacaagt
     1921 gggaccgtgg tcccagaccg ataatcagac cgacaacacg agtgggatcg tggtcccaga
     1981 ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac
     2041 gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccat
     2101 ggtcccagac taataatcag accgacgata cgagtgggac catggtccca gactaataat
     2161 cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg acgatacgag
     2221 tgggaccgtg gtcccagtct gattatcaga ccgacgatac aagtggaaca gtgggcccag
     2281 agagaatatt caggccagtt atgctttctg gcctgtaaca aaggacatta agtaaagaca
     2341 gataaacgta gactaaaacg tggtcgcatc agggtgctgg cttttcaagt tccttaagaa
     2401 tggcctcaat tttctctata cactcagttg gaacacgaga cctgtccagg ttaagcacca
     2461 ttttatcgcc cttatacaat actgtcgctc caggagcaaa ctgatgtcgt gagcttaaac
     2521 tagttcttga tgcagatgac gttttaagca cagaagttaa aagagtgata acttcttcag
     2581 cttcaaatat caccccagct tttttctgct catgaaggtt agatgcctgc tgcttaagta
     2641 attcctcttt atctgtaaag gctttttgaa gtgcatcacc tgaccgggca gatagttcac
     2701 cggggtgaga aaaaagagca acaactgatt taggcaattt ggctgtgttg atacagcggg
     2761 taataatctt acgtgaaata ttttccgcat cagccagcgc agaaatattt ccagcaaatt
     2821 cattctgcaa tcggcttgca taacgctgac cacgttcata agcacttgtt gggcgataat
     2881 cgttacccaa tctggataat gcagccatct gctcatcatc cagctcgcca accagaacac
     2941 gataatcact ttcggtaagt gcagcagctt tacgacggcg actcccatcg gcaatttcta
     3001 tgacaccaga tactcttcga ccgaacgccg gtgtctgttg accagtcagt agaaaagaag
     3061 ggatgagatc atccagtgag tcctcagtaa gcagctcctg gtcacgttca ttacctgacc
     3121 atacccgaga ggtcttctca acactatcac cccggagcac ttcaagagta aacttcacat
     3181 cccgaccaca tacaggcaaa gtaatggcat taccgcgagc cattactcct acgcgcgcaa
     3241 ttaacgaatc caccatcggg gcagctggtg tcgataacga agtatctcca accggttgag
     3301 tattgagcgt atgttttgga ataacaggcg cacgcttcat tatctaatct cccagcgtgg
     3361 tttaatcaga cgatcgaaaa tttcattgca gacaggttcc caaatagaaa gagcatttct
     3421 ccaggcacca gttgaagagc gttgatcaat ggcctgttca aaaacagttc tcatccggat
     3481 ctgaccttta ccaacttcat ccgtttcacg tacaacattt tttagaacca tgcttcccca
     3541 ggcatcccga atttgctcct ccatccacgg ggactgagag ccattactat tgctgtattt
     3601 ggtaagcaaa atacgtacat caggctcgaa ccctttaaga tcaacgttct tgagcagatc
     3661 acgaagcata tcgaaaaact gcagtgcgga ggtgtagtca aacaactcag caggcgtggg
     3721 aacaatcagc acatcagcag cacatacgac attaatcgtg ccgataccca ggttaggcgc
     3781 gctgtcaata actatgacat catagtcatg agcaacagtt tcaatggcca gtcggagcat
     3841 caggtgtgga tcggtgggca gtttaccttc atcaaatttg cccattaact cagtttcaat
     3901 acggtgcaga gccagacagg aaggaataat gtcaagccca ggccagcaag tgggctttat
     3961 tgcataagtg acatcgtcct tttccccaag atagaaaggc aggagagtgt cttctgcatg
     4021 aatatgaaga tctggtaccc atccgtgata cattgaggct gttccctggg ggtcgttacc
     4081 ttccacgagc aaaacacgta gccccttcag agccagatcc tgagcaagat gaacagaaac
     4141 tgaggttttg taaacgccac ctttatgggc agcaaccccg atcaccggtg gaaatacgtc
     4201 ttcagcacgt cgcaatcgcg taccaaacac atcacgcata tgattaattt gttcaattgt
     4261 ataaccaaca cgttgctcaa cccgtcctcg aatttccata tccgggtgcg gtagtcgccc
     4321 tgctttctcg gcatctctga tagcctgaga agaaacccca actaaatccg ctgcttcacc
     4381 tattctccag cgccgggtta ttttcctcgc ttccgggctg tcatcattaa actgtgcaat
     4441 ggcgatagcc ttcgtcattt catgaccagc gtttatgcac tggttaagtg tttccatgag
     4501 tttcattctg aacatccttt aatcattgct ttgcgttttt ttattaaatc ttgcaattta
     4561 ctgcaaagca acaacaaaat cgcaaagtca tcaaaaaacc gcaaagttgt ttaaaataag
     4621 agcaacacta caaaaggaga taagaagagc acatacctca gtcacttatt atcactagcg
     4681 ctcgccgcag ccgtgtaacc gagcatagcg agcgaactgg cgaggaagca aagaagaact
     4741 gttctgtcag atagctctta cgctcagcgc aagaagaaat atccaccgtg ggaaaaactc
     4801 caggtagagg tacacacgcg gatagccaat tcagagtaat aaactgtgat aatcaaccct
     4861 catcaatgat gacgaactaa cccccgatat caggtcacat gacgaaggga aagagaagga
     4921 aatcaactgt gacaaactgc cctcaaattt ggcttcctta aaaattacag ttcaaaaagt
     4981 atgagaaaat ccatgcaggc tgaaggaaac agcaaaactg tgacaaatta ccctcagtag
     5041 gtcagaacaa atgtgacgaa ccaccctcaa atctgtgaca gataaccctc agactatcct
     5101 gtcgtcatgg aagtgatatc gcggaaggaa aatacgatat gagtcgtctg gcggcctttc
     5161 tttttctcaa tgtatgagag gcgcattgga gttctgctgt tgatctcatt aacacagacc
     5221 tgcaggaagc ggcggcggaa gtcaggcata cgctggtaac tttgaggcag ctggtaacgc
     5281 tctatgatcc agtcgatttt cagagagacg atgcctgagc catccggctt acgatactga
     5341 cacagggatt cgtataaacg catggcatac ggattggtga tttcttttgt ttcactaagc
     5401 cgaaactgcg taaaccggtt ctgtaacccg ataaagaagg gaatgagata tgggttgata
     5461 tgtacactgt aaagccctct ggatggactg tgcgcacgtt tgataaacca gggaaaagat
     5521 tcatagcctt tttcatcgcc ggcatcctct tcagggcgat aaaaaaccac ttccttcccc
     5581 gcgaaactct tcaatgcctg ccgtatatcc ttactggctt ccgcagaggt caatccgaat
     5641 atttcagcat atttagcaac atggatctcg cagataccgt catgttcctg tagggtgcca
     5701 tcagattttc tgatctggtc aacgaacaga tacagcatac gtttttgatc ccgggagaga
     5761 ctatatgccg cctcagtgag gtcgtttgac tggacgattc gcgggctatt tttacgtttc
     5821 ttgtgattga taaccgctgt ttccgccatg acagatccat gtgaagtgtg acaagttttt
     5881 agattgtcac actaaataaa aaagagtcaa taagcaggga taactttgtg aaaaaacagc
     5941 ttcttctgag ggcaatctgt cacagggtta agggcaattt gtcacagaca ggactgtcat
     6001 ttgagggtaa tttgtcacac tgaaagggca atttgtcaca acaccttctc tagaaccagc
     6061 atggataaag gcctacaagg cgctctaaaa aagaagatct aaaaactata aaaaaaataa
     6121 ttataaaaat atccccgtgg ataagtggat aaccccaagg gaagtttttt caggcatcgt
     6181 gtgtaagcag aatatataag tgctgttccc tggtgcttcc tcgctcactc gagggcttcg
     6241 ccctgtcgct cgactgcggc gagcactact ggctgtaaaa ggacagacca catcatggtg
     6301 ctgtgttcat taggttgttc tgtccattgc tgacataatc cgctccactt caacgtaaca
     6361 ccgcacgaag atttctattg ttcctgaagg catattcaaa tcgttttcgt taccgcttgc
     6421 aggcatcatg acagaacact acttcctata aacgctacac aggctcctga gattaataat
     6481 gcggatctct acgataatgg gagattttcc cgactgtttc gttcgcttct cagtggataa
     6541 cagccagctt ctctgtttaa cagacaaaaa cagcatatcc actcagttcc acatttccat
     6601 ataaaggcca aggcatttat tctcaggata attgtttcag catcgcaacc gcatcagact
     6661 ccggcatcgc aaactgcacc cggtgccggg cagccacatc cagcgcaaaa accttcgtgt
     6721 agacttccgt tgaactgatg gacttatgtc ccatcaggct ttgcagaact ttcagcggta
     6781 taccggcata cagcatgtgc atcgcatagg aatggcggaa cgtatgtggt gtgaccggaa
     6841 cagagaacgt cacaccgtca gcagcagcgg cggcaaccgc ctccccaatc caggtcctga
     6901 ccgttctgtc cgtcacttcc cagatccgcg ctttctctgt ccttcctgtg cgacggttac
     6961 gccgctccat gggtattttc agtgttgcca ccatcgtctg cagctggctg acgtaccagg
     7021 agtcagagag cggaaccagc cggtgagtct gctgaccggc gggcattctc cccgccgtcc
     7081 tggcagcttt ttcggtccgt tgtttcaggg tcgcaagctg cacaaacgga tacggaggcg
     7141 caagcgaaaa atccccccgc gtcagcgcca gtgcttcatt aatgcgtgct ccggtgttcc
     7201 acagtgtggc cagcagcatc ttgcggtgca gatccgggac gtaatggagc agggcactca
     7261 cttccggagc cagcagatat tttggcagtt catcatggac catcgacatc tggcgaagtg
     7321 ccagagctgc cggataatca atggcaacag gcagcgatgc aggctgcccg gcagaataca
     7381 ctgccggtac catgactgca gactggctgt gtataacgga gcctgacatt tatattcccc
     7441 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
     7501 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
     7561 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
     7621 cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat
     7681 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac
     7741 cagtccctgt tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc
     7801 atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc
     7861 atggttgtgc ttaccagacc ggagatattg acatcatatg ccttgagcaa ctgatagctg
     7921 tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcac acctcttttt gacatacttc
     7981 gggtatacat atcagtatat attcttatgc cgcaaaaatc agcgcgcaaa tacgcatact
     8041 gctatctggc ttttagtaag ccttatgtat tttacctttc gttatgttaa ccaataaaaa
     8101 ttaaaatctg acttataaaa acaaagcgta attaccgcat tcccgtttcg tatggatccg
     8161 actttatttg aataaac
//
LOCUS       DAEVZA010000179         7935 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEVZA010000179 DAEVZA010000000
VERSION     DAEVZA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.179"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..103)
                     /locus_tag="LAF92_004541"
     CDS             complement(<1..103)
                     /locus_tag="LAF92_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI9793438.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMV"
     gene            complement(124..1416)
                     /locus_tag="LAF92_004542"
     CDS             complement(124..1416)
                     /locus_tag="LAF92_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI9793439.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQH"
     gene            complement(1430..3109)
                     /gene="ycjM"
                     /locus_tag="LAF92_004543"
     CDS             complement(1430..3109)
                     /gene="ycjM"
                     /locus_tag="LAF92_004543"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBI9793440.1"
                     /translation="MKQKITDHLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(3322..3636)
                     /gene="pspE"
                     /locus_tag="LAF92_004544"
     CDS             complement(3322..3636)
                     /gene="pspE"
                     /locus_tag="LAF92_004544"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBI9793441.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(3711..3932)
                     /gene="pspD"
                     /locus_tag="LAF92_004545"
     CDS             complement(3711..3932)
                     /gene="pspD"
                     /locus_tag="LAF92_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBI9793442.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(3941..4300)
                     /gene="pspC"
                     /locus_tag="LAF92_004546"
     CDS             complement(3941..4300)
                     /gene="pspC"
                     /locus_tag="LAF92_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBI9793443.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(4300..4524)
                     /gene="pspB"
                     /locus_tag="LAF92_004547"
     CDS             complement(4300..4524)
                     /gene="pspB"
                     /locus_tag="LAF92_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBI9793444.1"
                     /translation="MGALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(4578..5246)
                     /gene="pspA"
                     /locus_tag="LAF92_004548"
     CDS             complement(4578..5246)
                     /gene="pspA"
                     /locus_tag="LAF92_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBI9793445.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            5413..6390
                     /gene="pspF"
                     /locus_tag="LAF92_004549"
     CDS             5413..6390
                     /gene="pspF"
                     /locus_tag="LAF92_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBI9793446.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(6510..7775)
                     /gene="puuE"
                     /locus_tag="LAF92_004550"
     CDS             complement(6510..7775)
                     /gene="puuE"
                     /locus_tag="LAF92_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBI9793447.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAVEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
BASE COUNT         1912 a         2061 c         1910 g         2052 t
ORIGIN      
        1 acaccatcgg ccacgccacc agaccgccca gcagtaataa gctgggcgcg agaagcaaca
       61 gcgcaaaggg catatcggaa cgacctgaaa aaagcctgtt cattgcggat tcctgtcctg
      121 ggtttagtgc tgttcgacca gttcatccag ctttttctgg ctcgcttgca gcgtgccagg
      181 gagatcggct ttacccacgg tgacgttatg caccatggag ctcaccacgc cagaccccgt
      241 cacatcaccc atgcgggtaa agtttttatc ccctactgca ccaaagacct gaatatttgg
      301 cagttcaccg attagctgat tcggtagttc acccagcgcc ttaataacgt cgttgtcttt
      361 ccaggtggcg gtagtgacaa ccgctttatt caccggcagc gcagcacctg gcgacatcat
      421 cacccagtcg gcaatgttgt ctgcctgctc cataaaggtg acaaattttt ctgctgcttc
      481 cgtctcttcg gtcttttgcc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc
      541 agagtttttc tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac
      601 agccggaagg atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc
      661 tttcacttcc atgatgtcgt tagaacccgg catagtgttg gcagcaaggt cgcggtaata
      721 ggtcagtgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac
      781 gttagcctgg ttggataacg caaactggga gaaggattgt tccgtcaaca cgctttctgc
      841 tgtaggcagc gcaatgccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc
      901 cagcagctgt tgccagtttt tcggctcctc aagccccgct tttgccagca catctttgcg
      961 ataccagata ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc
     1021 ggtacgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac
     1081 tgtggcaacc gctttgcgat cgataagctg ctctttgtcc atcactttgg cgtagtcatg
     1141 gctggtttcg atcacttccg gcagcgaacc gctacgtgaa agagtaatga ctttagtgtt
     1201 ataggcatct tcttccacgg gcacttgctt aacggtgatg ccagggtttt ctttttcaaa
     1261 acgggcaatc aatttactga taacggcctg gcgctcctgc tccaccgaag aatgcataaa
     1321 ttcgatggat acattcgttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac
     1381 cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc
     1441 acataacctg ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca
     1501 gatcccgacc atgcagatta gtaatattta catgctgaat atttttactg acattaaaca
     1561 atccagtcag acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa
     1621 tcgtatcaat ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa
     1681 ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc
     1741 gggttatctc tttactgtga tattttttac ggttaatcgc acggttatat ccgagttttt
     1801 cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc gctggcacac
     1861 cggggaaact taacaaaatc gcatgggcga gaataaacct ggcgcaacgt tcttcatcgc
     1921 tactctcacg gcggcttaac gcatccatat aggtcacatt tatttcatac ggactgcgtg
     1981 taccgtcggg attatttttc cagtttacta atgcaccttc ctgctgtaac gcctcgacca
     2041 gctctaatat ttcgctttca ggcaacaagc cccgtagcgg gtttagcccg atgccatcgt
     2101 gagaggcgag gaagttaaac caggtggtgt tgctggaagg tagtgtcagg ttttgcgccc
     2161 acgcacaaag cgcctcaacg ttctgttttt gcaccgcatg cagcaccagt ggcggcagcg
     2221 agaactggta caccatatgt gcttcgtcat cgcctgcgcc aaagtaagca atgttgtctt
     2281 tatgcggaac attggtctcg gtaatgatca ctgtacctgg cgcaacgtta tcaataatcg
     2341 accgtaacag tttgataatc agatgtgttt tttccagatg gatgcagctt gttcccggct
     2401 ctttccacat aaagccaacg gcatccaggc ggacatactc agcacctttc gcaaggtaac
     2461 agagtaaaac atccaccatc gccagcaaca cttcagggct acggtaattc aggtcaattt
     2521 gatcgtcact aaaggtggtc cataaatggc gcgttgaatg atcgcgcatc tggaatggcg
     2581 ttaataacgg taacgcacgc gggcgagtga cggcgctgag atcggtttgc gggtcaacgg
     2641 caataaaaaa atcttcaaaa cctggatgct gttgtaaata gtttttaaac cattcacttt
     2701 ttgccgacat atggttgcag acaaaatcaa acattaaatg actgcattca ccgagttgct
     2761 gaatatcctg ccactccccc gcttcactgg cgacctgttg ataatcaatt accgaaaagc
     2821 catcatcaga tgaccatgga taaaacggca acaaatgaac atgtgaaaaa atgctttgca
     2881 gccattgatg gtaaaactga ttaaatgtgg gtaatggttt taaatcattg ctgtgaaatt
     2941 gatcggcata ggtaattaac acgacatcac tttcatccca gtgtttttta cgtcgctgtg
     3001 taattaatcg tttcgcactc tcaagacgcg ttacaagttt ctgtaaatga gttgcggtaa
     3061 atgttccacc gtagatttcg tccaggtgat ccgtaatttt ctgtttcatg ttgcctttcc
     3121 gtggtagcgg tcccatgaaa cgagagacta ctccgcagaa aataaactgt caattgagca
     3181 caagggtttt agtcacgcag gaaatcattt tttaccaggg aaaaagcgta tttgtgagct
     3241 gacgcaaata atctatgggg atgtaaataa ggtgtgacag aaaaaaaaac ggcgcataag
     3301 cgccgctcat ggtgaattct tttaaccttt gaccttcggc attgcgatgt ctttcaggcc
     3361 accggcgttc tcaacgtggg tatatcccat ctcgctaagg atctcttttg cttgccctga
     3421 ctggcgtccg gcattgcaat acactttcac ggtgtcgttt ttatccggaa cggcggtggc
     3481 aatgcgctct ttcacttcct tcaggggaat attgatggcc ccctgaacgt gctcttgctg
     3541 atactgctct ggaacacgaa catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa
     3601 caccagtgcc agagcaagta agcctttttt aaacatgtaa gcgtccttct atggatgaaa
     3661 attgccatcc attatacgcc tgagatttaa caatttttgc gaaactcccc tcacctttta
     3721 taacgctgtg ccagtttatt agcagcccga cttaacagcg gttccagcgc cacagccagc
     3781 aacattttca gcggtcggcg agcaactgat tttatcgccc agcccgccac acccgccggg
     3841 ccatagcgca gtgcggtaag aagtaccagc ttgcctgcta atttgaaacc aggctttacc
     3901 ttttgcccgg cctgttgcca gcgagtattc ataactttcc tcacagttga cggaaacggc
     3961 tacgtaacgt gaaagtatcg gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac
     4021 ttgccgccag ttcacggtcg acttcatcca gcaattcgct gctggaaggt agctgctcac
     4081 caaaggccat gttgtccggc attggatcaa gcgcaaatga caaaatgatg taagcaacca
     4141 gggtaaacag cgccagaccg aagaaaatcg acagcaccac caggatacgc accagtttta
     4201 ccggtacatc aaaatagttg gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg
     4261 gaatacgcca taatttttta ttgagattaa tgcccgccat tagcgatccc tccagttcgg
     4321 atgttctgca tcaagaatag attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc
     4381 atcagccagt tgcgctaatc gctgctgctc actttgcgac aattcactgc gaccagaacg
     4441 attgctgtaa tgcagccata accagatcgg taaaacaaac agcacaaaaa tggttaacgg
     4501 aatagccaga aatagcgcgc ccataagtac tccttagacg atgagcggcg gcgcgttcgg
     4561 acgccgcctg gatgttatta ttgattgtct tgcttcattt tggcttttaa ttgtgccagt
     4621 tgttcgctga ttgcatcatc ggctttcagt tcggcaaact gatcgtccag cgatttttgt
     4681 ttaccgaagc tgtggctttc tgcttccgct tccatctggt caatacgacg ttcgaaagat
     4741 tcgaaacgag ccattgcttc atccagtttg ccactgtcca gctgacgacg cacatcgcgc
     4801 gacgagtttg ccgcctgatg acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc
     4861 aatttgtttt ccagctcgcc aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc
     4921 gtcacttcat gttccaggga cttaatcaga tcggtcagtt tctgtttttc aattaacgct
     4981 gcacgtgcca gatcctctct ctctttcagc agcgccagtt cggctttttc ctgccattca
     5041 acttcacgcg ccgacgcttg ttcaatacgg cgagtcagct gtttcttttc tgccaacgcg
     5101 cgcgccgaag tagaacgtac ttcaaccagt gtatcttcca tctcctggat catcagacga
     5161 accagtttct gtggatcttc cgctttctct aacagagcgt tgatgttggc attcacgatg
     5221 tcggcaaagc gagaaaaaat acccataatg ttgtcctctt gatttctgcg ttcaggattg
     5281 tcctgctgaa ctgttaatac aatttgcgtg ccaatttttt atctttttga tttataaaga
     5341 tctgattgaa gaatcaacag caacatgcca ggatgagtta gcgaattaca ctaacaagtg
     5401 gcgaatttca tcatggcaga atacaaagat aatttacttg gtgaggcgaa cagctttctc
     5461 gaagtgctgg aacaggtttc gcatctcgca ccgctggaca aaccggtgct catcatcggc
     5521 gaacgcggca ccggtaaaga gctgattgcc agccgcctgc attatctctc ctcccgttgg
     5581 caagggccgt ttatttccct taactgcgcg gcgttaaatg aaaatctgct ggattccgaa
     5641 ctgtttggtc acgaagcggg ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt
     5701 gaacgtgccg acggcggtac gctatttctt gatgaactcg ctacggcacc gatgatggtg
     5761 caggagaaat tattgcgcgt gattgagtac ggtgaactgg agcgcgttgg cggcagccag
     5821 ccattgcagg tgaatgtgcg gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc
     5881 aatgaaggca cttttcgcgc tgacctgctc gaccgactgg cttttgatgt tgtacaactg
     5941 ccaccactgc gcgagcgcga aagcgacata atgttgatgg cagaacactt tgccatccag
     6001 atgtgtcggg aaatcaagct gcctctgttc ccggggttta cggagcgcgc cagagaaaca
     6061 ttgctgaatt atcgttggcc gggaaatatt cgtgaattga aaaacgtggt ggaacgttca
     6121 gtgtatcgcc acggcaccag cgattatccg cttgatgaca tcattattga tccctttaaa
     6181 cggcgtccgc ctgaagacgc tatcgccgtt tcagaaacca cctcgcttcc aacactgccg
     6241 ctggatttac gtgagtttca gatgcagcag gaaaaagagt tgctgcaact cagtttgcaa
     6301 caagggaaat ataaccagaa gcgcgcggct gaattactgg ggttaactta tcatcagttc
     6361 cgcgcgttgt tgaaaaagca ccagatttag cgaagttgag caatacaact tgccggatgc
     6421 ggcgtgaatg cttttttcgg catacataat caatacgtcg tttgcaggcc tgataagcgc
     6481 agcgcatcag acattattgc gttgggctat taatcgctca gcgcatcctg caaaattttc
     6541 attgccgcat cgaattgcgc atccgggatg gtcagcggat acaggaagcg aatcacgttg
     6601 ccgtatgcgc cacaggtcag caggagcagc ccctgcgcca gcgcgcgttg ctggattttc
     6661 tgtgcaatcg ccgctgacgg ctcgcccgtt tgcggatcgt taaactctac cgcaatcatc
     6721 gaccccaggc cgcgtaccgc agcaatggcc ggaacgcttt ctttggcatc aatcaacgtg
     6781 tttttaagac gctggcccag ttgattcgcg cgttcgcaga gtgattcttt gtcgataata
     6841 ttgagcaccg cgtgcgcggc agccaccgcc agcgggttac cggcgtaggt gccgccaagc
     6901 ccgcccggcg cgggtgcgtc cataatattc gcgttaccga ccacgcccga aagcggcatc
     6961 ccgcccgcga ggcttttcgc catcgtcatt aaatccggct tatcggcgta atgatccatg
     7021 gcaaacagct taccggtacg cgcaaagccg ctttgcactt catcagcaat catcacaata
     7081 ccgtgctcgt cgcacaggcg gcgaatagcg gcaaccagct cttttggcgc aacgttgaaa
     7141 ccgccctcgc cctgcaccgg ttcgaaaata atcgccgcca cctgcttcgc ttcgatgtct
     7201 gatttaaaca agcgttcgat ggcgtcgagg gagtcctgtg ttgaaatgcc gtgtaaatct
     7261 gacggataag gtacgtgata caccgaacca gggaacgggc cgaagccgat tttgtacggc
     7321 gcaacttttc cggtcagcgc catggtcata tacgtacgac cgtgaaagcc gccgctaaac
     7381 gcaatcacgc cagggcgtcc ggtatgggcg cgagcaattt tcaccgcgtt ttccaccgct
     7441 tccgcaccgg tggtgaagaa cgcggttttg gcctgcccgc tcaccggggc aagggcgttg
     7501 attttctccg ccagggtgac gtagctttca tacggcacaa tctgatacgc ggtgtgggta
     7561 aactgttgca gttgctgctc caccgccgcg accagatcag ggtggcgatg tccggtattc
     7621 agcaccgcaa tgcctgcggc gaaatcgatg tactcgttgc cctcaacatc cttcagcgtg
     7681 gcgttttcag ccgactgggc gaagaagtta cacatcacgc caaccccgcg cggggtggca
     7741 gaaagacgac gctgatggaa ttcattgttg ctcatgtttc acaccttttg actcttcacg
     7801 gttaatcgga aattaaacgc ccagacgatc gcgcaggctg taatacgccg cgcccatcgc
     7861 ggtaaacggc acacgcagcg tgcgcccgcc ggggaacggg taatgcggca gattggcgaa
     7921 ggcatcgaaa cgttc
//
LOCUS       DAEVZA010000180         7877 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEVZA010000180 DAEVZA010000000
VERSION     DAEVZA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.180"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(102..845)
                     /locus_tag="LAF92_004551"
     CDS             complement(102..845)
                     /locus_tag="LAF92_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793448.1"
                     /translation="MTDIQAATESIVAATSHPVSVQLINQGAGIWGNVATGLITGLLT
                     GGITLTGIWLTHYFTLKRERQASEDKMKKELHYIATELVFMLERYAEGCFRVVTDDGQ
                     DDDAPQPERKAVTNYPELNLIDVSGDWRTLEPRLMYRIRELPVLQDEAHRAIAYAAEY
                     SDPPWHKDYFRERQYQFTRLGINAVILAVRLRKATGMPETRLTGHDEWSAVSVFRKVW
                     RRERALRAAEATRNREWNQLVIPDGMSNQ"
     gene            complement(896..1246)
                     /locus_tag="LAF92_004552"
     CDS             complement(896..1246)
                     /locus_tag="LAF92_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793449.1"
                     /translation="MFTAFNERNDFSYAFEKIRNAISAPGENNLYAATELGLGILLRK
                     YEQFRQELDAAGELGNWEYDLDTYNHCIAVLQRYFTGNPSGLTERDARIYSHYLQTEH
                     KRFVKLAEELAAGR"
     gene            1798..2886
                     /gene="pifC"
                     /locus_tag="LAF92_004553"
     CDS             1798..2886
                     /gene="pifC"
                     /locus_tag="LAF92_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor PifC"
                     /protein_id="HBI9793450.1"
                     /translation="MLSQLNLRFHKKLIEALKTRAGRENTSVNALAERFLDDGLKTVA
                     PGDGYFQLIADPEATVRQLYRHIILGQTFGTSALSRDELRFVLVHVREAFLRGHNRLA
                     TLPALDTLLDITGNLLAWQVEHDRPVDGHYLKGIFRLAGKNWTEEFEAFRAALRPVVD
                     QMYAEHLLRPLESDCFGLAEVPDAVLAEIFTLPRLKAVFPLMLRGLDWNTEQARTLAQ
                     ELRPVISAVTETIEAGTLRLEIRVDGQHPGERPGAWYTTPRLHLLITGQDFVVPYGWE
                     ALSELLGLFTLYARHPEALTHGHQGERVMFSPPGNVTPEGFFGIDGLRIFMPAEAFET
                     LVRELATRCQEGPLAEALTGLRCLYGDL"
     gene            2888..5113
                     /locus_tag="LAF92_004554"
     CDS             2888..5113
                     /locus_tag="LAF92_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage T7 exclusion protein"
                     /protein_id="HBI9793451.1"
                     /translation="MKILRQLWNQKGLDAAVEDVPEDRYGFGNIAENISRSILTLPLE
                     ASNVVGIEGAWGSGKTSLLNLILRNLALKKDAHTHVLHISPWLSGGSPVEALFLPVAT
                     VIQQEMEIRYPPKGFKKLWRKYLLSPEAQKVIEYAQDTSSRVLPLVQYIGQFSSIINW
                     IAGGIKVFSDSRLAVDQKTTTKLRAEIAGQLVSLDLKFIVVMDDLDRLEPSQVAEVFR
                     LVRAVADLPRFTHILCYDRQIITHAVEHALNIEDGSRYLQKIIQLSFKLPRPEAFDLR
                     NEFRQRAEALYQQINNQPPDSGMVRDLIAVTDTYGAALSTPREIHQAINSLIFLYPGM
                     RDFVYFPDLCLLQLIRVTNPALYDWTEHYLTERSVIETGQGMLSDGEKADFREGLIRC
                     MKTFRASNADSFLTLADWIPGISGHNDEYLNLFEPVSEDFRHIQTTGKRLSSLTHWRY
                     YFAFSSPQNVLPPEFFRQLFEQAGVSEKQQQLSELLLSKINSVGSLSGTWFEHILSRL
                     TPGLIRERNFEECAGLVHFFFDHTDEVSTRFSIRNPWFSLREMAINEVVRHLLKHMQD
                     IDETRTITLMEKLIVTGASPFWIADFMRDLIWEHGLAQNAVPSPSDALFSRDITERLR
                     DRFAERMNQPELQQQLLLRKSLLGYLYAWRDMSSGETVKQWVREVTTTDEGLVNLLIR
                     LQTSVFSSHRGAYRRIARDQVSPFFDDWPAVEEKLKVMLSGNELTPEQEALKTALEND
                     D"
     gene            complement(5163..6062)
                     /locus_tag="LAF92_004555"
     CDS             complement(5163..6062)
                     /locus_tag="LAF92_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HBI9793452.1"
                     /translation="MAIDIFQSLKNCVFDLQRADVQNYQQPLKQLARLLNSENLQSVN
                     AHLTRNVELDTFLARSEDTESSMAGSAVLQWPDEPADILGLKLLLIEKMADDNNFSFN
                     FCHTFFYDRNIIESIRKFTSSLVAPFVRDYQLYVENQHDPEPAVFRPVSRKIFIVHGH
                     DNDALQSVARFISRIGLEEIILSERPDGSRTIIEKFEAESGDVSFAIVLMTPDDSGSA
                     LASESTRLRARQNVLYELGYFAGKLGRGKVLVLRKGDIEIPSDLAGVLYTELDEHGGW
                     KRKLLSELAYAGVPFDKDKALSA"
     gene            complement(6052..6342)
                     /locus_tag="LAF92_004556"
     CDS             complement(6052..6342)
                     /locus_tag="LAF92_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793453.1"
                     /translation="MSVKITGLDKMQKQLKEVERATEALNGSYDVHFDANDPVSIENA
                     IQEAYSMVYERASGYATNPMVSPLIEHMKENLRQQILDRAEQQRQESGQDGN"
     gene            6638..6868
                     /locus_tag="LAF92_004557"
     CDS             6638..6868
                     /locus_tag="LAF92_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBI9793454.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            6865..7281
                     /locus_tag="LAF92_004558"
     CDS             6865..7281
                     /locus_tag="LAF92_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HBI9793455.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(7443..7748)
                     /locus_tag="LAF92_004559"
     CDS             complement(7443..7748)
                     /locus_tag="LAF92_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793456.1"
                     /translation="MDSFDYPRLQALVSSPDEIKNLYRRCRNGYEKLQVFRLLELDQD
                     HPVIRKFVNETYHIENEFICQLDPSRFDLIPEYVIMECDKLIALPPAANQSSVARIA"
BASE COUNT         1946 a         1996 c         2013 g         1922 t
ORIGIN      
        1 ttgaacgagc gccatctggc gcagggccag cgcggcggga tagtcaatgg ccaccggcat
       61 ttgcgccggc tgctgcgggt gagaatatga taagaggccg ctcattggtt tgacattcca
      121 tcaggtatca ctaattgatt ccactccctg ttgcgggtag cttcagcagc gcgcagggct
      181 ctttcgcgcc tccagacttt acgaaacacg gaaactgcag accattcatc atgacctgta
      241 agacgtgttt cgggcatccc ggttgccttg cgcagacgga cggcaagaat gacagcgttg
      301 atgcccaagc gcgtgaactg atactgccgt tccctgaaat agtccttatg ccaaggaggg
      361 tcggagtatt cggcggcata tgcaattgcc ctgtgggcct catcctggag caccggcagt
      421 tcacggatgc gatacataag acgtggctct aaagtccgcc agtcaccgga gacatcgatc
      481 agattcagtt cagggtaatt cgtgacagct tttctctcag gctggggggc atcatcatcc
      541 tggccgtcat ccgtcacaac ccggaaacaa ccctcagcat accgttcaag catgaatacc
      601 aactcggtgg caatgtaatg cagttccttt ttcattttat cttctgacgc ctgtctctca
      661 cgcttaagcg tgaaataatg tgtcagccag atgccagtga gtgttatacc cccagtaagc
      721 aggccagtga taagcccggt tgccacgttt ccccagatcc ccgccccttg attaatcagc
      781 tgtacggaaa ccggatggga tgtcgcagca actatactct cagttgcggc ctgtatatca
      841 gtcattgatc atttacccct ttatattcct gagaaatccc gccctctgtc cgtagtcagc
      901 ggccggcagc cagctcttca gccagtttca caaacctttt gtgctcagtc tgcaggtaat
      961 ggctgtaaat ccgggcatca cgttctgtca gtccggatgg attgccggtg aagtaacgtt
     1021 gcagcaccgc aatacagtga ttgtaggtat cgagatcata ttcccagttt cccagctcac
     1081 cggccgcatc cagttcctgg cgaaactgct cgtatttacg gagcagaatg ccaagaccta
     1141 attctgttgc agcataaaga ttattttctc ctggcgcgga aatcgcatta cggatttttt
     1201 caaaagcata actgaaatca tttctctcat tgaatgcagt aaacatgtcc tttcctcagt
     1261 tgaatagtta gccgtgcaat tttaccacac ctcaatcatc gtcagatgag tgccactctg
     1321 tcgtgacagt gactttcact agagtgattt tcacatacta aaataataag cagccatata
     1381 acaacgtaat taaattacct gacggccgct ctggccggat ccgactttta tgttattcca
     1441 cagtttccgg aagtgtacca cgcacacgga ataaccacag cgccctttca gggtggtgag
     1501 cggccggtca cggggcaggg gagggacaca acatatatgg tcactgacag ctggaaacac
     1561 cgctacatct tgtgtttccg ggccgctgcg cgccggcggg gctgaatcag atccgtaact
     1621 tggggttttc ggatctccgg agaaataaga agaggcaggc attgttgtag cattaaaatc
     1681 ctcaatttta gtaattttag aagcatcaca attttcaaaa actaaaaaac tgattaatag
     1741 ctaataataa aatgctaggt tagcattaag aacaaaacaa acattaaagg atcgatgatg
     1801 ctaagccagc ttaacctgcg ttttcacaaa aaacttatcg aggcgctgaa aacgcgtgcc
     1861 ggtcgggaaa atacttcggt caacgcccta gccgaacgct tcctcgatga cggcctgaaa
     1921 acggtcgcgc ccggtgacgg gtattttcag ctcattgccg atccggaggc cacggtccgg
     1981 cagctgtacc ggcatatcat tctgggccag accttcggca cgtcagcgct ctcccgcgac
     2041 gaactgcgct ttgtcctggt tcacgtcagg gaggccttcc tgcgcgggca taataggctg
     2101 gccacactcc cggccctgga cacgctgctg gacatcactg gcaacctgct ggcctggcag
     2161 gtggagcacg atcgccctgt ggacggtcac tacctgaagg ggattttccg cctggccggg
     2221 aaaaactgga cggaagagtt tgaagccttc cgggcagcgc tgcgccccgt ggtggatcag
     2281 atgtatgccg aacacctcct gcgtccgctc gagagcgact gttttggcct ggcggaggtg
     2341 ccggacgcgg tgctggcaga aatcttcacc ctgccgcggc tgaaagctgt tttcccgttg
     2401 atgctgcgcg gcctggactg gaacacagaa caggcaagaa cgctggcaca ggagctgcgc
     2461 ccggttattt ctgccgtcac ggaaaccatt gaggccggca cgctgcgcct ggagatccgc
     2521 gttgacggcc agcaccccgg agaacgcccg ggggcctggt acaccacacc ccgtctgcac
     2581 ctgctgatca ccggccagga cttcgtggtg ccgtacggct gggaagcgtt atccgaactg
     2641 ctgggcctgt tcacgctgta tgcccggcat ccggaagccc tgacgcacgg tcaccagggg
     2701 gagcgagtga tgttctcccc gcccggaaac gtcacgccgg agggcttctt cgggatagac
     2761 ggcctgcgga tttttatgcc ggcggaggcc tttgagacgc tggtccggga gctcgccacg
     2821 cggtgtcaag aaggcccgct ggcggaggcc ctgaccgggc tgcgctgcct gtacggagat
     2881 ctgtaatatg aagatactga ggcagctgtg gaaccagaaa gggcttgatg cggcagttga
     2941 agatgtacct gaggatcgat acggtttcgg taatatcgca gaaaatatat cccgttccat
     3001 ccttactctt cctctggagg cttcaaatgt tgtcggaata gaaggtgcat ggggatcggg
     3061 taaaacaagc ctccttaatc tgatcctgag aaaccttgcc ctgaaaaaag atgctcacac
     3121 gcatgtgctg catatttctc cctggctgag tggcggcagt ccggttgaag cgctctttct
     3181 tccggttgcc acggttatcc agcaggaaat ggaaatacgc tatcccccga agggcttcaa
     3241 aaagctctgg cgtaaatatc tgttgtcacc ggaagctcaa aaagtgattg agtatgctca
     3301 ggatacttca tcgcgcgtcc ttccactggt tcaatatatc ggacagtttt ccagcattat
     3361 taactggata gcaggcggaa taaaggtatt ttcagacagc cgtctggctg ttgatcagaa
     3421 aaccacgaca aagcttcggg ctgaaattgc aggacaactg gtgagtctgg atctgaagtt
     3481 cattgttgtc atggatgatc tggaccgact ggagccatcc caggtggcgg aagtgttcag
     3541 gcttgtgcgt gcagtagccg atctgccccg ctttacccat attctctgtt atgacaggca
     3601 gattatcact catgccgttg aacatgcgct gaatatcgaa gatggcagcc gttatctcca
     3661 gaaaatcatt cagcttagtt ttaaattacc ccgacctgaa gcctttgatt tacgtaatga
     3721 atttcgccag cgggctgagg ctctatatca gcaaattaat aatcaaccgc cagactctgg
     3781 aatggtaagg gatctcatcg cggtgactga tacctatggt gccgcacttt cgacgccacg
     3841 ggaaatccat caggccatta attccctgat ttttctttat ccggggatgc gggattttgt
     3901 ttatttccct gatttgtgcc tgcttcagct tatacgggtg acaaacccgg ctctgtatga
     3961 ctggacagag cattacctga cagaacggtc cgtgattgaa accggtcagg gtatgctttc
     4021 tgacggagag aaagcagact tccgggaggg gcttatcaga tgtatgaaga cgttcagggc
     4081 atcaaatgca gactcgtttc tgacacttgc agactggatc ccaggtatca gtggacataa
     4141 tgatgaatat ctgaatcttt ttgagcccgt cagtgaggat tttcgtcata tccagacgac
     4201 cggtaaacga ctcagcagcc tgacgcactg gcgatattat tttgccttct cctcaccaca
     4261 gaatgttctg ccacctgaat tcttcagaca gctttttgaa caggctggcg tatcggaaaa
     4321 acagcagcag ctttctgaat tgctgctctc aaaaatcaac agtgtgggca gtctgtccgg
     4381 aacctggttt gaacatatac tgagcagact gactccaggc ctgataagag aacgtaattt
     4441 cgaagagtgc gcaggactgg ttcatttttt cttcgatcac accgatgagg tgagcactcg
     4501 cttcagtatc cgtaacccat ggttttccct gcgtgaaatg gccattaatg aggttgtacg
     4561 gcatctgctg aagcatatgc aggatattga tgagaccaga acgatcacgc ttatggaaaa
     4621 actgatcgtt acgggtgctt cgccgttctg gatagccgat tttatgcggg atcttatctg
     4681 ggaacatgga cttgctcaga atgcagtacc ttcgccttca gacgctcttt tcagtcgcga
     4741 tattacggaa cggttgcgtg acaggtttgc ggaacgtatg aatcaaccag aacttcagca
     4801 acagcttctc ctgcgcaagt ctcttctcgg gtatctctac gcctggagag acatgagttc
     4861 aggtgaaacc gtgaagcaat gggtcagaga agttactacc actgatgaag gccttgttaa
     4921 cctgttgata cgattacaga ccagtgtctt cagctcccac cgaggggcat atcgccggat
     4981 tgcgcgtgac caggtcagtc cgttctttga tgactggcca gcagtcgaag agaagttgaa
     5041 ggttatgctg tccggcaacg agctgactcc ggagcaggaa gcgctcaaaa cagcgctgga
     5101 aaatgacgac tgaccaaaaa ttaaggccgc ttagcggcct ttttcttact aaatgaagga
     5161 gatcaggcgc ttaatgcttt atccttgtca aaaggaacac cggcatacgc aagctcactc
     5221 agcagtttgc gtttccatcc accatgctca tcaagctcgg tatagagaac cccggcaaga
     5281 tctgaaggga tttcgatatc tcccttcctg agtacaagta ctttcccgcg tccaagcttc
     5341 ccggcgaaat atccgagctc atacaggaca ttctggcgtg cgcggagacg tgtgctttca
     5401 gatgccagag cactgccgct atcgtcgggc gtcatcagca cgatggcaaa actgacatcc
     5461 cctgactctg cttcaaattt ctcgataatc gtcctggagc cgtcaggacg ttctgaaaga
     5521 atgatttcct caagacctat ccgggatata aaacgggcaa cagactgaag ggcatcatta
     5581 tcatggccat gaacaataaa aatttttcgg gaaaccggcc gaaacactgc aggctcagga
     5641 tcgtgttggt tttccacata caactggtaa tcgcgcacga acggggcaac taatgacgac
     5701 gtgaattttc gaatggattc aataatgttt cgatcataga aaaatgtatg acagaaatta
     5761 aaggaaaaat tgttatcgtc tgccattttc tcgatcagca gcaacttcag gcccaaaata
     5821 tcagcgggct catcaggcca ctgtagaaca gcggaaccag ccatgctgga ttcagtatct
     5881 tcactcctgg ccagaaaggt atcaagttca acattgcggg tcagatgtgc attaactgac
     5941 tgcagattct ctgagttcaa gagccgggcc aattgcttaa gaggttgctg atagttctga
     6001 acgtcagcgc gctgaaggtc gaatacgcaa tttttaagtg actgaaagat atcaattgcc
     6061 atcctgcccg ctctcctgtc tttgttgttc tgctctgtcc agaatctgtt ggcgcagatt
     6121 ttctttcatg tgttcgatta atgggctgac catcggatta gtggcataac cagaagcgcg
     6181 ttcgtacacc atggaatagg cttcctgaat cgcgttctca atgctgacag gatcgttagc
     6241 atcgaagtgg acatcataac ttccgtttaa tgcctctgtt gctctctcaa cctcttttag
     6301 ctgtttctgc attttgtcta aacctgtgat tttgacgctc ataacaacct ccggactaag
     6361 tgagatgtat caacctttat agatagcacg ttggagatgt ggggtaaggg atatcactca
     6421 aattccatgt aatttcatta agatcagact taaccttttt tcggatctcc gataaaaaag
     6481 aaagaaatag agatatgcta aacatatgcc tggcatctac atatgctacc gctatacata
     6541 tgccacacat ctgcctatac taaacatatg cacacacccg gtgctttgaa tattccggat
     6601 atgtttcaca tatccatatg ctcgggagag aaaaaacatg agaaccgtat cgatattcaa
     6661 aaatggcaac aaccgcgcca tccgtctgcc tcgtgatctg gattttgagg gggtgagcga
     6721 gctcgagatc gtccgggaag gggacagcat catcctgcgc cccgtccggc caacctgggg
     6781 atcattccta gagtacgaaa aagccgatcc agattttatg gcggagcgcg aggatgttgt
     6841 cagcgacgaa gggcgattta acctgtgaac aagacttata tgcttgatac ctgtatctgc
     6901 tcgttcatca tgcgtgaaca gccagaagcc gtcctgaagc gtctggagca ggcggtgcta
     6961 cgcggccacc gtatagtggt ctcggccatc acctactctg aaatgcgctt cggggctacc
     7021 ggcccaaagg cctcaccacg tcacgtccaa ctggtcgatg aattctgcgc ccgcctcgat
     7081 gccatcctgc cctgggatcg cgccgcagta gacgccacca cgaagattaa agtggcactg
     7141 cggctggccg ggacgccgat cggcccgaac gacacggcga ttgccgggca cgccatcgcc
     7201 gccggggcga tactggtaac gaataataca agagaatttg agcgagtgcc tgacctggtg
     7261 ctggaagact gggtgaagta agacactctt ttcaaagtca ggagataagg gcaccaagaa
     7321 acagcccgta tacgggctgt tttaacagat atctacagta agcggtccta aactgtaggg
     7381 tatcttcgaa gatcagacta tcagggagct aaagcctatc ttccggcggc aaaaacagtg
     7441 gctcaagcga tacgggcaac cgagctctgg tttgctgccg gtggcagggc gatgagcttg
     7501 tcacattcca taatcacgta ctccgggatg agatcgaatc tggacggatc tagttgacag
     7561 atgaactcat tttcaatgtg gtacgtttcg ttaacgaatt ttcgtattac cggatggtcc
     7621 tgatccagtt ccagtaaacg gaatacctgc agtttttcat agccgttacg acagcgacgg
     7681 tacaggtttt ttatttcatc tgggctgctg acgagagcct gaagtcgcgg gtaatcaaaa
     7741 ctgtccacat actcccggat atcccgcaag gcctgctgaa ttttctcagg agccattttg
     7801 ggatagcccg agcccgcagc ggcaggctcg cgataatcca gcggggcgac gcgtcgatgg
     7861 aatagattgg atagaag
//
LOCUS       DAEVZA010000181         7861 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEVZA010000181 DAEVZA010000000
VERSION     DAEVZA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.181"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..668
                     /locus_tag="LAF92_004560"
     CDS             <1..668
                     /locus_tag="LAF92_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBI9793457.1"
                     /translation="IFTFSFGFILALALAKKNIRFAKVWRLIFILPYAIPTFVTLLIF
                     RLLLNGIGPVNSFLNSIGINSVDFLSVPLNAKITVIAVCVWVGAPYFMLLIAGALTNI
                     SRDLYEASEVDGATKWQQFKEITLPMVLHQVAPTLVMTFAYNFNNFGAIYLLTEGGPT
                     NPEYRFAGHTDIFITWIYKLTLNFQQYHIASVISIIIFIFLSVIAIWQFRRMKSFKDD
                     VGM"
     gene            671..1504
                     /locus_tag="LAF92_004561"
     CDS             671..1504
                     /locus_tag="LAF92_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBI9793458.1"
                     /translation="MDKILRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLIVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVSFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            1521..3320
                     /locus_tag="LAF92_004562"
     CDS             1521..3320
                     /locus_tag="LAF92_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBI9793459.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEYHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISTPECEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNGRPEISPEGVEPWGSTPTSFNFMGGDLWGVIDKLDYLEDLGINGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGVDSPI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEG
                     TPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLL
                     DSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRKCM
                     IWDENKQDLELRQFIRWLIRLRKKHPQWCEASIQWKDVEHPTVIAYQRDNITFFLNNS
                     EDTANFIYDGRSMEISGFSYEIEGLPAADLYDF"
     gene            3342..4139
                     /locus_tag="LAF92_004563"
     CDS             3342..4139
                     /locus_tag="LAF92_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBI9793460.1"
                     /translation="MIIDAHTHIGDFVKISMPEDVFLASLDKYDIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQAIKICGSDKILFGTD
                     NPINGLDTYNDDDFYNKYFTELKSKLTQSEFEQLMFTNAKKLFKIKI"
     gene            4232..5071
                     /locus_tag="LAF92_004564"
     CDS             4232..5071
                     /locus_tag="LAF92_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI9793461.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPRNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
     gene            5141..5935
                     /locus_tag="LAF92_004565"
     CDS             5141..5935
                     /locus_tag="LAF92_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBI9793462.1"
                     /translation="MVAVSQPTVDKIIFGAEATLINKIGTCWIASMDVLRKAVFEGVN
                     IIITHEPTFYSYADLEGDVLEFSWARKIMDYTRGELSYLKIIEQKKEFLHKNNLVIIR
                     CHDVMDREPTFGMSKALAQQLELDVTNIVASDDMYHVYAIEPDSAINITKRFAKNLKI
                     YNLPGIQFYGDRARIVRTIGIGAGCFCEPIQYMEYQADYYITINDSIKTWVQTQYSKD
                     SGLPMAVIGYSVAEEAGMRRLASYLDLHSGYPCIHFTAGCDYDWIE"
     gene            complement(5995..7437)
                     /gene="pyk"
                     /locus_tag="LAF92_004566"
     CDS             complement(5995..7437)
                     /gene="pyk"
                     /locus_tag="LAF92_004566"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBI9793463.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAARLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(7565..>7861)
                     /gene="hexR"
                     /locus_tag="LAF92_004567"
                     /gene_synonym="yebK"
     CDS             complement(7565..>7861)
                     /gene="hexR"
                     /locus_tag="LAF92_004567"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBI9793464.1"
                     /translation="VELAQLARENDAMVIALTSAGTPLAREATLAITLDVPEDTDIYM
                     PMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKESRFDKQLLNLSDDR"
BASE COUNT         2173 a         1506 c         1737 g         2445 t
ORIGIN      
        1 cgatttttac cttcagtttt ggctttattt tggctttagc actggctaaa aaaaatatac
       61 gtttcgcaaa ggtctggcga ttgatattta tactgccata tgccataccc acatttgtaa
      121 cgttacttat tttccgcctt cttttaaatg gaattggtcc ggttaactcg tttctgaaca
      181 gtattggaat taattcagtt gattttctgt cggttccatt aaatgcaaaa ataacagtaa
      241 ttgccgtttg tgtttgggta ggggcaccgt attttatgct attgatcgcg ggtgcattaa
      301 ccaacatatc ccgagatttg tatgaggcta gtgaggtcga tggcgcaact aagtggcaac
      361 agtttaagga aattacattg ccgatggtgt tacatcaggt tgcgccaact ctggtaatga
      421 catttgcata caacttcaat aattttggtg caatttatct tctgactgaa ggtggcccga
      481 caaatccgga atatagattt gctgggcata cagatatttt cattacctgg atttataaac
      541 ttacattgaa tttccagcaa tatcatatag cgtctgtgat ctctattata atctttatct
      601 tcttatcggt tatagcaata tggcaattca gacggatgaa atcatttaaa gatgatgtag
      661 ggatgtaact atggataaaa ttttacgtaa aatatcaacg ctcttagttt atcttttttt
      721 aatctgcaat tccattcttg tgcttgggcc agtgatatgg acgataatgg catcttttaa
      781 aaaagggaat aacctattta gttcaacatt tagtggcatc gagtttacat ttgaccacta
      841 cataacgttg ttcacggata cgccatatat gcagtggtat ttgaacactt tcattcttgc
      901 aaccgctaac atgttaattt cactgatcgt tgtgacgatg accgcctacg ttttttcaag
      961 gtatcgattt cgtggaaaac gtaatacttt aatgagtatt cttgtattgc aaatgtttcc
     1021 tgcattttta tcaatgactg ccatttatat cctgctatca aaggccaatt tgatagacac
     1081 atatactggg ttgcttctgg tttatgtaac cggttcattg cccttcatga cgtggttggt
     1141 aaaaggttat tttgatgcta ttccaacttc tttggatgag gccgctaaaa tagatggagc
     1201 agggcatctg actattttta ttcagataat attgccattg gctaagccga ttctcgtttt
     1261 cgttggatta gtttcgttca ctggaccatg gatggacttc attttgccaa cgttggtgct
     1321 tcgtagtgag gataaaatga ccctggcgat tggaattttc agttggattg cttctaattc
     1381 agcagaaaat tatacgcttt ttgctgctgg ggcactttta gttgcagtac ctatcacatt
     1441 gttatttgtt ttcacacaga agcatataac tactggactt gttagcggtg ccgttaagga
     1501 ataattaggg attagtattt atgattacag ttagttcgtt aaaacactca gcaaaaagtg
     1561 aagatagcta tgcctatgat aacgaaactt tgcatgtacg attacgcaca ttaagaggag
     1621 aagttgataa agttattctc tggataggcg atccatataa ttgggctgaa ggtggtcttg
     1681 acggcggtaa catggccggg acagaagcat tcggctggat tggcggcaac gagatcgaga
     1741 tggaacaaga agctgtcacc gaatatcatg atcactggtt tgcagtgttc aaacctcaaa
     1801 aacgccgttg ccgttacggt tttatacttt ttgggaaaga gggagagaag tttctttttg
     1861 gtgagaaaag atgtgttgat atcagtacac cggagtgcga agagcgagag ttaagccgct
     1921 taaataactt tttttgcttc ccttatctta ataaaattga cgttctaaat accccctctt
     1981 gggtaaaaaa tacggtatgg taccaaattt ttccagatcg tttctgtaac ggcagacctg
     2041 aaatatcccc agaaggggta gaaccgtggg gcagtacacc gacttcattc aactttatgg
     2101 gtggggatct gtggggggtt attgataaac tcgattatct ggaagatctg ggtattaatg
     2161 ggatttattt ttgtcctatt tttacctcag cttccaacca taaatatgac acaatcgatc
     2221 actttagtgt tgatccacat ttaggcggga atattgcttt ccatactttg attgaagaag
     2281 cgcataagcg tggaataaaa atcatgttgg atgcagtatt caatcattta ggcgttgact
     2341 ctccaatatg gcttgacgtt gtacgcaatg gtgccaattc acgttacgcc gattggttct
     2401 ggattcataa atttcctgtt tatcccgata ctccaaaaag tgaatgggat tttaaaaact
     2461 ttaactatga aacctttggc aatgttattg aaatgcctaa gctaaatact gagaatgaag
     2521 agtgccgtga atacttactt agtatagttc gctattggac gcaaaatttt gatattgatg
     2581 gttggcgtct ggatgttgcg aatgaagtgg atcatcattt ctggcgagat ttcagaaagg
     2641 tgattaaaga tatcaaacct gaatgttata ttttgggtga gatatggcat gagggcaccc
     2701 cctggttgcg tggtgatcag tttgattcat taatgaatta tcccttaaca tatgggatta
     2761 ttgattattt tgcgttacag gatacaacaa aacaagaatt tatgacatcc gttacacgtt
     2821 cttatctttg ttatcctaaa aatataactg aagttatgtt taatcttctg gatagtcatg
     2881 acaccgcgcg tattttaagc gtttgtttga atgataaacg aaaagttaaa ctcgcctatt
     2941 tatttatgtt aactcaagcg ggctctcctt gtatctacta cggtagcgaa ataggaattg
     3001 atggttttaa atcaatgacg cttgagaata atagaaaatg tatgatatgg gatgaaaaca
     3061 aacaagatct tgaattacgg caatttatac gttggttaat acgtctacgt aaaaaacatc
     3121 cacaatggtg tgaggcatca atacaatgga aagatgttga acatcctacg gtgatcgctt
     3181 atcaacgcga taacatcact ttcttcttaa ataatagtga agatactgcc aactttattt
     3241 atgacggaag aagtatggag atctccggtt tcagttatga aattgaaggt ttacctgctg
     3301 ctgatcttta tgatttttaa atttcaatta ggatgtttgt tatgataatt gatgcacaca
     3361 ctcatattgg tgattttgtg aaaatcagca tgccagaaga tgtttttctg gcatctctgg
     3421 acaagtatga tattgatttt gcgttatgtt cctgtggatc tgccgttgaa gttgaccatg
     3481 accagaaccc gattccggat gaagatcaag ttacacagca tgataataat gaacgtatgc
     3541 tcagacttgt tcgtcaacat tcaaagcgaa ttggcgcgtt tatgtggatt aagcctcgtc
     3601 tggaaagttg tgatcaagat tttgaagata tgattgccag caatcgagat atcatttatg
     3661 gcatcaaagt tcacccatat cattcaaaaa tggctttcaa ttcagataaa gttcaggaat
     3721 atatacgcct tgctcaaaaa tatgatctcc cggtagttag ccatacttct aatgattatg
     3781 aatccagccc acagttggtt tatgagatgg cacttaaata tccaaatgtg aattttgtta
     3841 tgtgtcatat ggggctcgcg acagataatc aggaagcgat cgaattgatt gcaaaacttc
     3901 ctaatctcta tggcgatact gcgtgggtta gggcggatat ggtttaccag gcgataaaaa
     3961 tctgtggtag tgataagatt ttatttggta ccgataatcc tattaatgga ctagatactt
     4021 ataatgatga tgatttttat aataagtatt ttactgaatt aaaaagtaaa ttaacgcaaa
     4081 gtgagtttga acaacttatg tttaccaatg cgaaaaaact ttttaagata aaaatatagt
     4141 tattagtcat ttctaagtgc tccccatata gaatgcggta ggggagtgct tagatgttat
     4201 atttcatttt gcattgaggt taatttaaat catgaacgat attgagaact atggtaatcg
     4261 ctatcatgtg ttgcaatcct ttcctatgcc acaattggca agtagtcgcg aaataagaat
     4321 attactacct gcgtcctatt atgaatctga tgtcagttac cctgtattgt atatgcacga
     4381 tgggcaaaat ttatttgata aatctgtatc ttttggtcca catgtatggg atatacctga
     4441 ggcggtagat gcttttttcc caaattccca atacgacggt gttatcattg tggggattga
     4501 taatgcatcg tctcatggta aattcagccg tatggatgag tattctccat ggccgcgaaa
     4561 cacatctttt cccttacctg gctgggaccc tgatgtggat tatagtggag gaaaaggtgc
     4621 gttgtatgtt gattttattg tgaatacgct caagaactat attgacagca attttcgaac
     4681 tctcccagat cgtaataata cagcgatagc aggtagttct atgggtgcgt acatcagttt
     4741 gtttgccgca attcttcgtc tagacgtttt ttcaaaagtt ggcgtttttt cgccggcact
     4801 atggttcaat gacagcgcaa tgctgaattt tattcaggaa aacaatatct ttgcagatct
     4861 tactgtatat ctcgatgtcg gtacgctgga aaccagtggt atgcgcgaag actttcctga
     4921 agtctatatt tcaggcgcgg aaaaattatg cgtgagtctc cgtaaacaac gcaatgtaac
     4981 tatagattat catctatggg gaggtgatac ccactctgag tctgcatggg caaaacgttt
     5041 tccagaaatg ttgaaattat tttattgctg atgaaaccgg tgaaggatgt tgaatgcatc
     5101 atattaaaac agccgcagat cttaattgtt tttaaattca atggttgccg tttcgcaacc
     5161 aactgttgat aaaattattt ttggtgctga ggcgacactt attaataaaa ttgggacctg
     5221 ctggattgcc tcaatggatg ttttaagaaa ggctgttttt gaaggtgtca atatcattat
     5281 tactcatgag cctacatttt attcttatgc tgatctggag ggtgacgttc ttgagttttc
     5341 atgggccaga aaaataatgg attatacgcg cggtgaactc agttatttaa aaataattga
     5401 gcaaaaaaag gagtttttgc ataaaaataa tttggtaatc attcgttgcc atgatgtcat
     5461 ggatcgagag cctacgttcg gaatgtctaa ggctttggct cagcagttag aactggatgt
     5521 tacaaatatt gttgcttctg atgatatgta ccatgtatat gctatagaac ctgacagtgc
     5581 cattaatatt accaaacgtt ttgccaaaaa tttgaaaatt tataatcttc ctgggatcca
     5641 attttatgga gatagagcca gaatagttag aactattggg attggggcag gctgtttttg
     5701 tgagccaata caatatatgg aatatcaggc tgattattat attacgatta acgattcaat
     5761 taaaacatgg gtgcagacac aatactccaa agatagtggc ttaccgatgg cagttattgg
     5821 atatagtgtt gcagaggaag cgggtatgcg acgtcttgca agctatttag atctccattc
     5881 aggctatccc tgtattcatt ttacagcggg ctgtgattat gattggattg aatgagtagt
     5941 cctaatttat gtgttgctaa ataccccggt catcagcggc cggggcaggt gagattactc
     6001 taccgttaaa atacgcgtgg tattagtaga acccacggta ctcatcacgt cgccctgggt
     6061 gacaatcacc aggtcaccag acatcaagta acctttatcg cgcagcagat taaccgcttc
     6121 gctggcagct gctacgccgt cattagcgct atcaaagtgc accggcgtaa cgccacgata
     6181 gagagcagtc aggttcagcg tacgttcatg gcgcgacatg gcgaaaattg gtagaccaga
     6241 gctgatacgg gaggtcatca gcgcggtacg acccgattcg gtcatggtaa tgatcgccgt
     6301 aacgcctttc aggtggttag ctgcgtacat tgctgacatg gcaatagctt cttccacatt
     6361 gtcgaactga acgtccagac ggtgtttaga aacgttgatg ctcgggattt tttccgcacc
     6421 caggcaaacg cgcgccatgg ctgcaacagt ttctgacgga tactgccctg cggcagtttc
     6481 tgcagacagc atcacagcgt cagtaccatc cagaacggcg tttgctacgt ccatgacttc
     6541 tgcacgcgtc ggcatcgggt tagtaatcat tgactccatc atctgggtcg ccgtgattac
     6601 cgctcggttt agctgacgcg cacgacggat caacgctttc tgaatgccga ccagttccgg
     6661 gtcgccaatt tccacaccga ggtcgccacg tgcaaccatt accacgtcag aggcgaggat
     6721 gatgtcatcc attgcatcct ggctgcaaac ggcttccgca cgttcaacct tggcaacaat
     6781 tttcgcatca catcctgcat cgcgtgccag gcgacgggca tagttcagat cttcgccaca
     6841 gcgtgggaag gagacagcca ggtaatctac gccaatcaac gccgcagtct taatgtctgc
     6901 tttgtctttt tcggtcagcg cttcagccga caaaccgccg ccaagtttgt tgataccttt
     6961 attgttggag aggggaccac cgacggtgac ttcggtgaac actttcatgc cctgaacttc
     7021 cagtactttt aactggacgc gaccatcgtc cagcagcagg atgtcaccag gcacgacgtc
     7081 agcaggcagg cctttgtagt cgataccgac tttttctttg tcgccttcac ctttacccag
     7141 gttggcgtcg agcaggaatt tatccccaat attgaggaaa actttgcctt ctttaaaggt
     7201 ggatacacgg attttgggcc cctggaggtc acccagaata gccacatgac gccccagtct
     7261 tgcggcaatc tcacgaactt tatccgcgcg cattttgtga tcttcaggcg agccgtgaga
     7321 aaagttcata cgtacaacgt tggcacccgc cgcgataact ttttcaagat tattatcgcg
     7381 atctgttgct gggcctaacg tggtaacgat ttttgttctg cgaagccttc tggacatgta
     7441 atactccgtt gactgaaaca accaggtgtt gcttgaacat gaaatccgaa tgaaataacg
     7501 ccgcgatggc gataaattaa tctcattcgg atgtcgtata agattaggac agtgacagtc
     7561 gtttttagcg atcgtcactt aaattaagta actgcttatc aaaacgcgat tccttcagcg
     7621 cttccttgac ccgcttcaag ttatctctga attttgcacc gcgtcgcaaa gtaaatcctg
     7681 tcgccagcac atctatcacg gtcagctgtg caagtcgaga aaccatgggc atataaatgt
     7741 cagtatcttc cggtacgtcg agggtaattg ccagcgttgc ttcccgggcg agcggggtac
     7801 ccgcagaggt gagggcaatc accattgcgt cgttttcgcg agccagctgc gccagctcga
     7861 c
//
LOCUS       DAEVZA010000182         7785 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEVZA010000182 DAEVZA010000000
VERSION     DAEVZA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.182"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            326..892
                     /locus_tag="LAF92_004568"
     CDS             326..892
                     /locus_tag="LAF92_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI9793465.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            943..3402
                     /locus_tag="LAF92_004569"
     CDS             943..3402
                     /locus_tag="LAF92_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI9793466.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPVISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEKGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3417..4148
                     /locus_tag="LAF92_004570"
     CDS             3417..4148
                     /locus_tag="LAF92_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI9793467.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPTALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            4145..5206
                     /locus_tag="LAF92_004571"
     CDS             4145..5206
                     /locus_tag="LAF92_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9793468.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5358..6404
                     /locus_tag="LAF92_004572"
     CDS             5358..6404
                     /locus_tag="LAF92_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBI9793469.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6401..7192)
                     /gene="nei"
                     /locus_tag="LAF92_004573"
     CDS             complement(6401..7192)
                     /gene="nei"
                     /locus_tag="LAF92_004573"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBI9793470.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7228..>7785)
                     /gene="pxpA"
                     /locus_tag="LAF92_004574"
     CDS             complement(7228..>7785)
                     /gene="pxpA"
                     /locus_tag="LAF92_004574"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBI9793471.1"
                     /translation="FPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRHV
                     KPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVFA
                     DRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLHG
                     DGEHALAFARRLRSTFAEKEIVVAA"
BASE COUNT         1979 a         1988 c         1841 g         1977 t
ORIGIN      
        1 ctaaacacaa aaaattgtaa aaataaaatc cactatcgga cattcatata ttctaaaaaa
       61 aaatatatcg gtataaatta taaacaaatc gatgattttt aatcatctat cttcctctca
      121 gttacatgaa taaaccacca aatatagcgc acccctataa tttccaatac tttattcatc
      181 aaacatttaa ttcataccat caattaatta cttttacatt taataaaatt aataatattt
      241 ttaagaaaaa ctaaaaacat tcgacaatca aataagatat tcttgcaccg atatactttt
      301 ttcttttctg ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc
      361 cgtatctctg ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca
      421 ctttaaaggt gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga
      481 ggttgaactc ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt
      541 tgctgtcgac ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg
      601 caagatttct aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc
      661 gattctcaac aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa
      721 agatcaaagc aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc
      781 cagcgaacaa acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt
      841 aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact
      901 ttggcggcgc tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt
      961 tatcgactct cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa
     1021 ttcaacctta atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag
     1081 gaaaaaggag tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa
     1141 atcagtaacg ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc
     1201 aatgatttac tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg
     1261 ataaatcagt gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc
     1321 aatcagcaac taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg
     1381 accccaccct ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt
     1441 gccagcagct accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca
     1501 gccggaatca acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat
     1561 agcgatgata accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta
     1621 ccgcaattag gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac
     1681 ggtttttctt ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta
     1741 cgcggctacg ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt
     1801 caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac
     1861 cttaatcagt ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg
     1921 aacaatttcc aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc
     1981 tataaactgg ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc
     2041 ttttttagca atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg
     2101 ctgctttctg gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg
     2161 cttggcgcgc tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac
     2221 gagcggggct taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg
     2281 atttccctcg ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctt
     2341 gatcacaaat acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg
     2401 ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg
     2461 tggaatgcag acgcctccac gaccgccaac atcacagccg gttttaatgt tgatattggt
     2521 gactggagag atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat
     2581 cgcgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc
     2641 tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg
     2701 gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga
     2761 gcccaggtga gcggtaacta tcagcatctg agttcagcgg gtgagtggga tatttctggt
     2821 acctatgccg ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca
     2881 acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc
     2941 agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt
     3001 ggcattgcgg tggtgccggt gatttccagt taccagcctt ccaccgtggc ggtgaacatg
     3061 aatgacttac ccgacggagt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa
     3121 ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt
     3181 cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc
     3241 attagcgtgg ggatggttgg cgagaaagga catgcctggt taagcggagt cgctgaaaat
     3301 caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat
     3361 atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga
     3421 catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac
     3481 tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg
     3541 tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag
     3601 gggagaaaag cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag
     3661 caacaacgca ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggtgatcgcg
     3721 aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc
     3781 atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccgactgcat
     3841 tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc
     3901 aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg
     3961 aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca
     4021 caaacaccgg aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg
     4081 cgttacgcat gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga
     4141 agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa
     4201 gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga
     4261 agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc
     4321 tgacgatatt aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga
     4381 gcatgtttat gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg
     4441 cgtaacttac aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa
     4501 ccagtgtatt gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg
     4561 atggcagaac aaaatttgct cgggtgatcc cgctaatatt cacatgtcgc gtacctttct
     4621 cgctcgcatg cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac
     4681 gcttagcgac tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagaccctac
     4741 tgcccaaaac ctgaaatatc atattactgg tctggaaaac attcgcgtgc tggattgcag
     4801 cgtcaatttt tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct
     4861 ggatattcgt cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa
     4921 tgaccaatgc accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt
     4981 tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa
     5041 cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt
     5101 ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc
     5161 tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc
     5221 ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat
     5281 tgctgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa
     5341 taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc
     5401 tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga
     5461 tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc
     5521 tcgccgcaca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca
     5581 ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca
     5641 gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct
     5701 ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag
     5761 atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg
     5821 ttctggtcac ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct
     5881 ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta
     5941 cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg
     6001 cagtgctcca gtctggtcag ctcatcacca tcgaactacc ggaatggctg ctggcgatgg
     6061 cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg
     6121 cactacgccc gctaccgcaa atcctgctgt cgatttttgc tctgctggct atttgtgcgg
     6181 gtatggcgtg ggggctgacc cggtttatgc acattgattt tatgactgcc tacctcgcca
     6241 ccagtcccgg cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg
     6301 cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca
     6361 ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagcctggac
     6421 accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc
     6481 acagttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct
     6541 catccacctg cccccgcgta gcgtaagaaa accgaggaat ctccagtaac gcgtgtgcga
     6601 gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct
     6661 gccagaggat ctccacccgc aaataattgc caagcccagc cagaaacgcc tgatcgagca
     6721 gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc tttaccacct
     6781 ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg
     6841 tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt
     6901 cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa
     6961 ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat
     7021 gagttaacaa cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg
     7081 attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga
     7141 tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat
     7201 ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct tttcggcaaa
     7261 tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat gcaggcagac
     7321 tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac
     7381 catctctagc gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc ctggctgact
     7441 tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt
     7501 tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaac
     7561 caatgctgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt
     7621 cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc
     7681 acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg
     7741 cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaa
//
LOCUS       DAEVZA010000183         7723 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEVZA010000183 DAEVZA010000000
VERSION     DAEVZA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.183"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..245)
                     /locus_tag="LAF92_004575"
     CDS             complement(<1..245)
                     /locus_tag="LAF92_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBI9793472.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQ"
     gene            406..2121
                     /locus_tag="LAF92_004576"
     CDS             406..2121
                     /locus_tag="LAF92_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBI9793473.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            2118..3611
                     /locus_tag="LAF92_004577"
     CDS             2118..3611
                     /locus_tag="LAF92_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBI9793474.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(3658..4107)
                     /locus_tag="LAF92_004578"
     CDS             complement(3658..4107)
                     /locus_tag="LAF92_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793475.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            4217..4564
                     /locus_tag="LAF92_004579"
     CDS             4217..4564
                     /locus_tag="LAF92_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBI9793476.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            4554..4916
                     /locus_tag="LAF92_004580"
     CDS             4554..4916
                     /locus_tag="LAF92_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBI9793477.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            4913..5410
                     /locus_tag="LAF92_004581"
     CDS             4913..5410
                     /locus_tag="LAF92_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBI9793478.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(5418..6602)
                     /gene="emrD"
                     /locus_tag="LAF92_004582"
     CDS             complement(5418..6602)
                     /gene="emrD"
                     /locus_tag="LAF92_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBI9793479.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(7021..7110)
                     /gene="tisB"
                     /locus_tag="LAF92_004583"
     CDS             complement(7021..7110)
                     /gene="tisB"
                     /locus_tag="LAF92_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBI9793480.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(7398..>7723)
                     /locus_tag="LAF92_004584"
     CDS             complement(7398..>7723)
                     /locus_tag="LAF92_004584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793481.1"
                     /translation="DSAVGSSFALNMEVVIGDLVGFVFNNIYTSTALQGRQWKIFQQG
                     KIEGLITFCTELQITKNPAGARFHQPPAAGNRKAHCIMSTLKVYVFRAERAISARILT
                     YLGHS"
BASE COUNT         1887 a         2011 c         2001 g         1824 t
ORIGIN      
        1 atttgttcgc cattctttaa aagaattttc ccacatcctt gtacggtata caccgcaacc
       61 catgagttgg cgataagcgg tttcttcttg tcccgcggcc agatcacccg gtaatgctcg
      121 tcggcgtagg tatgccagag cgagatcaga gaaattccgc tggagatcac attttcgtta
      181 atcaataagg gaatattgta cggagtttcg ttttttacat ctgaactttg caattttcca
      241 ttcattttaa tatccctccg gcgaattatg ctcacaacct gaacttaaca gaaaacaatc
      301 cgttcaggga tacgacgttt gtattttaag aatctgactg cctgacccga cgcattttac
      361 ctctccctat attcatgcgt ccgggacata aataagggct atgagatgaa ttcgttacaa
      421 atcttgagtt ttgtcggttt tacgctgctg gtggcggtga tcacctggtg gaaggtccgc
      481 aaaacagata ccggatcgca acaaggctat tttcttgccg gacgttcact aaaagcgccg
      541 gttattgccg cttcgttaat gctaaccaac ctttccacgg aacaactggt cggcctttcc
      601 gggcaggcct acaaaagcgg catgtcggtg atgggctggg aagtgacttc agcggtgacg
      661 ctgatcttcc tcgcgctaat ctttttaccg cgctatctga agcgcggcat tgccaccatc
      721 cccgattttc tggaggaacg ttatgataaa acgacgcgta ttatcatcga cttctgcttc
      781 ctcattgcca ccggcgtctg ctttctgccg attgttctct actccggcgc gttggcgctc
      841 aacagcctgt ttcacgtcgg ggaatcgcta cagatttctc acggtgcggc tatctggcta
      901 ctggtaattt tgcttggtct ggcgggaatt ttgtatgcgg tgatcggcgg actgcgcgca
      961 atggcagtgg cggactccat caacggtatt gggctggtta tcggcgggtt gatggtgccg
     1021 gtatttggcc tgatcgcgat gggcaagggc agctttatgc agggcattga gcaactcacc
     1081 accgttcacg ccgagaaatt aaactcaatc ggtggcccga ccgatccctt gccgattggc
     1141 gcggcattta ccggtttgat tctggtgaac accttttact ggtgtacaaa tcagggcatc
     1201 gtgcaacgca cgctggcgtc aaaaagcctg gcggaagggc aaaagggggc gctgttaacg
     1261 gcggtgctga aaatgctcga cccgctggta ctggtgctgc cagggttgat tgcgtttcat
     1321 ctgtatcagg atttaccgaa agccgacatg gcctacccga cgctggtcaa taacgttctg
     1381 ccagtgccaa tggtgggttt cttcggcgcg gtgttatttg gtgcggtgat cagtaccttc
     1441 aacggctttc tgaatagcgc cagtacgtta ttcagtatgg gtatttaccg tcgcatcatt
     1501 aaccagaatg ccgagccgca gcagctggtc accgtcgggc gcaaatttgg tttctttatc
     1561 gctatcgttt cggtgctggt cgcgccgtgg atcgccaacg cgccgcaggg gctgtatagc
     1621 tggatgaaac agctcaacgg catttacaac gtgccgctgg ttaccatcat cattatgggc
     1681 tttttcttcc cgcgcatccc ggcgctggcg gcaaaagtgg cgatggggat tggcataatc
     1741 agctacatca ccatcaacta tctggtgaag ttcgacttcc atttcctcta tgtgctggcc
     1801 tgtacgttct gcatcaacgt ggtagtgatg ctggtgatcg gttttatcaa accgcgcgcc
     1861 acgccgttca ccttcaaaga tgcgtttgcg gtggacatga aaccgtggag aaacgtcaag
     1921 atcgcgtcaa ttggcatcct gttcgcgatg attggcgtct atgccgggct ggctgaattc
     1981 ggcggctacg gtacgcgctg gttagcgatg atcagttatt tcattgccgc cgtagtgatt
     2041 gtctacctga tttttgacag ctggcggcat cgtcacgacc cagccgtaac ctttactccc
     2101 gacgcgaagg atagcctatg aaacgcccca attttctgtt catcatgacc gatacccagg
     2161 ccaccaatat ggtcggttgc tatagcggta aaccgctgaa tacgcaaaat attgatagtc
     2221 tggcggcgga aggtattcgc tttaattccg cctacacctg ttcaccggtt tgtacgcctg
     2281 cacgcgccgg actatttacc ggtatctacg ctaaccagtc cggcccgtgg accaacaacg
     2341 tcgcgccagg caaaaacatc tccactatgg ggcgctactt taaggatgcc ggctatcaca
     2401 cctgttacat cggcaaatgg catctcgacg gtcatgacta tttcggcact ggcgagtgtc
     2461 cgccggagtg ggacgctgat tactggttcg atggggcgaa ctaccttagc gaactgacgg
     2521 aaaaagagat cagcctgtgg cgcaatggcc taaacagcgt tgaggattta caggcgaacc
     2581 atatcgacga aaccttcacc tgggcgcacc gcatcagcaa tcgggcggta gattttctgc
     2641 aacagcccgc gcgcgccgag gaacccttcc tgatggtggt ttcgtatgat gagccgcatc
     2701 acccgttcac ctgtccggtg gagtatttag agaaatacgc tgatttttac tacgatctgg
     2761 gcgagaaagc tcaggatgac ctggcgaaca aaccggaaca tcaccgctta tgggcgcagg
     2821 cgatgccatc gccagtcggt gatgacgggc tttatcacca tccgctctat tttgcctgta
     2881 atgactttgt tgatgaccaa atcggacggg tcatcaatgc cttaacgcca gagcaacgtg
     2941 aaaatacgtg ggtcatttat acttccgatc acggcgaaat gatgggcgca cataagctga
     3001 tcagtaaagg agcggcgatg tatgacgaca tcacccgtat tccgctgatc atccgttcgc
     3061 cgcaagggga gcggcgacag gtcgatacgc cagtcagtca tatcgattta ctgccgacaa
     3121 tgatggcgct ggcagatatt gaaaaaccag agattctgcc gggggaaaat atccttgccg
     3181 tgaaagagcc acgcggtgta atggtggaat ttaaccgcta cgagattgag catgacagct
     3241 ttggcggttt tattccggtg cgttgctggg tgacggatga ctttaaactg gtactcaacc
     3301 tcttcaccag tgatgaactt tacgatcgcc gtaatgaccc aaatgaaatg cataacctga
     3361 tcgatgatat ccgttttgca gacgttcgca gcaaaatgca tgacgcctta ttggattaca
     3421 tggacaaaat tcgcgatccg ttccgcagtt accaatggag cctgcgtccg tggcgtaaag
     3481 atgcactgcc gcgctggatg ggggcatttc gtccacgccc acaagatggc tattcgccgg
     3541 tggtacgtga ctatgacacc ggcctaccga cgcaaggagt gaaagtggaa gagaaaaaac
     3601 agaagttctg acgataatgc ccgctgatga acacccggcg ggcattattc aggctgctta
     3661 aaccagtgaa ccgccggtca ggaaggaaaa aatcaggaat atcagcaaaa tgctgcacac
     3721 gatgccgata ccaaaaagca aggtatggaa agccagcgtc ccgccgccaa aaaccagcgc
     3781 gccgcgaatg ccccagcgat tttccttacg cataccgcca ataaatgcgc aaagtagcgc
     3841 agcaatggcc agtgcgatac cgctttgatc gaggatttta tcgacatcta cgtcctcttt
     3901 tttctccacg gtggtaattt tttcgccttt tattccggcg agaagacctc ttttaatttc
     3961 agccgttttg tccgccacga tgctttcaaa cgttggcggt ggtgcggaaa aagggccgaa
     4021 tgaaaagtgg ataatcccca tcattaaagc gatggcaccg aacagcatgc ccaatgaact
     4081 gattttattt tgagctatga tacccatctg acctccttgt agcggatggc gaaagcatat
     4141 caggaaacgg gcggacgaaa ttcttaaaaa aaaaccgcta aaggtagagc gttagtgaaa
     4201 ttgcaaagga gcaacaatga aaatttcccg ccttggagaa gcgcccgatt accgcttctc
     4261 gctggctaat gagcgtactt ttctggcgtg gatccgtacc gcactaggat ttctggcggc
     4321 gggggttggg cttgatcaac ttgcgccaga tttcgccacg cccgtcattc gtgaactgct
     4381 ggcgttgttg ttgtgtctgt tttccggcgg gttagcgatg tatggctacc tgcgctggtt
     4441 acgcaatgaa aaagcgatgc gtctgaaaga ggacctgccc tacaccaaca gcttattaat
     4501 cattagctta atattgatgg tcgtggcggt gatcgttatg ggactggtgt tgtatgccgg
     4561 atagccgcaa agccagacgc attgccgacc caggactgca gccggagcgc acatcactgg
     4621 cgtggtttcg taccatgctg ggctacggcg cgttgatggc gttggctatc aaacacaact
     4681 ggcaccaggc gggcatgtta ttctggattt cgattggcat tctcgccatc gtggcgctga
     4741 tcctctggca ctacacccgt aatcgcaatt taatggatgt cacgaatagc gatttttccc
     4801 aatttcacgt agtccgtgac aaatttttga tctccctcgc ggtgttatct ctcgcaatac
     4861 tgtttgctgt aacgcatata catcaactta tcgtatttat tgagagagtc gcatgacagg
     4921 aagtcaggtc attgatgctg aagaggacag gcataaactg gttgtagagt acaaggatgc
     4981 tctgcaacct gccgattttt atcacaattt caaacagcgg ggcatccgct ccgtacagct
     5041 cattccctat cttgaatttg atgaccgggg cgatctgacg gccgcttcgg tgacagcaga
     5101 gctgtggggg aaatttttaa ttgccctgtt cgagtgttgg gtgcgggcag atatcagtcg
     5161 tatctcgatt gagctttttg acgccacctt acaaaaatgg tgtggcagcg aaaacccgca
     5221 accgcgccgt gactgccagg cgtgcgactg gcatcgcctg tgcccgcacg ctcgccagga
     5281 gacgccagac agcgtgcttt gtgcgggcta tcaggcgttt tattcttact ccgcaccgca
     5341 tatgcgggtg atgcgtgatc tgataaagca gcatcgttct cccatggagc tgatgacgat
     5401 gctgcggtga cgtgcgctta aacgggctgc ccctgatgcg acatccgcgt cgccagcggc
     5461 agccagcaca gcacgatcaa caatcccatt aaggtcatca acaaccccag gctgccctga
     5521 ccggtttgcg gcaacatcgc agagagcgac gccagcacgc cggaaccaat gttttgcaga
     5581 ccgccgacca gcgcgccagc cgtgcccgcc aggaagggga acggctccat cgcgccgctg
     5641 gtcgccagcg gaaacagcat cccggcaccg aaaaagaaca gcgcggcggg aacgagcagc
     5701 gtccagacat tcatcacgcc aaaccagtcg gggatccaca tcagcaagcc agccagcagg
     5761 cagcagataa ccgactgcca cattaacgtg gagaagcgtt tattgggacg tccggcaaac
     5821 catgcgccaa aaaacgctgc cggaatcggc agaataaaca aaatactgac cgtcatactg
     5881 ctcagcccta acaccgcgcc catcagcacg ccggagcagg cttcaaaggc ggcaatcccg
     5941 gccagaccgc caatcagcat cagcaaataa cagttaaaac cgctgttacc gaaaagcgtt
     6001 ttataactgg taagcaggcg cgtgcgcggt gcatcgaccg gacgcgtttc cggcatccag
     6061 cgggccatac tgaaggtcac accagcacaa agaaccaaca aaaagagata acaggcgcgc
     6121 cagttccaca tcgtatccag cagaccgccg attagcggtg cgagcaacgg actgacgaga
     6181 atccccatgt ttaacaggct gttagcatgg cgcaactgtg tccgttcata taaatctcgc
     6241 ggcaaagtac gcgccattac gccgccaacg ccggtgccca tcccctgcat cgcgctggcg
     6301 gcaatcaaca ccgtcaaact ggaggtcgtg accgcgacca gcgttgccag cataaaaatg
     6361 gacattccga cgaggatcac cggtcggcgg cccacgcggt cggaaatcgg gccataaaac
     6421 agctgtgaga caccgtaagt cagcagataa gcgcccatta cgctctgcac cgccccttca
     6481 cggacgttga gatcgcgcgc catatcggca atagctggaa tataaatggt ttgcgccatc
     6541 tgaccgacgg ccacgagtaa taccaacatc aataacaaat tgacgtttct ttgccttttc
     6601 attatcacgg atgcttttat aaaaataatt agaataagtc actacgcatt cccataaatg
     6661 cgggagagga atctatcaca ttgatgaatg ttagccagat atacgccagg aatggggaat
     6721 tgtttagcgg cagaatatgt aaacaaaagc ggcaataaat gttgccggga gggattacgt
     6781 gcgaactgta accctctccc caaaggggcg agggggaaac caccgttccc tttcagaaaa
     6841 tgagctaaac attgcacggg acggtcccct cgcccctttg gggagagggc tagggtgagg
     6901 ggcacaggca gcgcaggtgc aaacattctg ctggtagaag aagcccccaa ctccaacccg
     6961 cacgctaaat ataaaagggg agcggttacc cgctcccctt tggtgcgact tgaatctgaa
     7021 ttacttcagg tatttcagaa cagcatcaag cagttgcagt gctgcaacaa tgagtttgag
     7081 gataagaatg gcgatatcca ccaggctcat acgcgtctcc tgtggttcag gagcactgcc
     7141 agctgacgta cctttccgct gcctgttgcc agctgttgtg cacgcggtat cttaaggaga
     7201 aacgttgtgc ttgttgacat aacacagtgt gctcacgcgg ggttaaggcg ctgacggcac
     7261 cacccgtttc agccaggact tagtgcgccg ggtaacgatg cggcccccgc ataacacatt
     7321 gcgtacagtg atattataga agtttactgt ataaataaac agtaatattt ggacaaaacg
     7381 caaactgtga tcgatactca actatgccct aaatacgtca aaattcgtgc cgaaattgcg
     7441 cgttctgcgc ggaacacgta tactttcagt gttgacataa tacagtgtgc tttgcggtta
     7501 ccagccgcag gcggctgatg aaacctcgct ccggcggggt tttttgttat ctgcaattca
     7561 gtacaaaacg tgatcagccc ctcaattttc ccttgctgaa aaattttcca ttgtctcccc
     7621 tgtaaagctg tgcttgtata aatattgtta aacacaaaac caacaaggtc cccaatgact
     7681 acttccatgt tcaacgcaaa actactacca actgcgctat ccg
//
LOCUS       DAEVZA010000184         7702 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEVZA010000184 DAEVZA010000000
VERSION     DAEVZA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.184"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..452
                     /locus_tag="LAF92_004585"
     CDS             <1..452
                     /locus_tag="LAF92_004585"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="catalyzes the transfer of phosphoglycerol to the
                     glucan backbone; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerol transferase I"
                     /protein_id="HBI9793482.1"
                     /translation="LKIVDNDIRYKADSFIFNVAGAPEEVKQFSGISRPESWGRWSNA
                     QLGDEVKIEYKHPLPKKFDLVITAKAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLH
                     FDNPTDADTLVIVPPEPVSTNEGNILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(591..1613)
                     /gene="lgoD"
                     /locus_tag="LAF92_004586"
     CDS             complement(591..1613)
                     /gene="lgoD"
                     /locus_tag="LAF92_004586"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HBI9793483.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            1752..2666
                     /locus_tag="LAF92_004587"
     CDS             1752..2666
                     /locus_tag="LAF92_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI9793484.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            2881..4242
                     /locus_tag="LAF92_004588"
     CDS             2881..4242
                     /locus_tag="LAF92_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9793485.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNQDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(4291..5946)
                     /gene="tsr"
                     /locus_tag="LAF92_004589"
     CDS             complement(4291..5946)
                     /gene="tsr"
                     /locus_tag="LAF92_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBI9793486.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(6065..6511)
                     /gene="hpaR"
                     /locus_tag="LAF92_004590"
     CDS             complement(6065..6511)
                     /gene="hpaR"
                     /locus_tag="LAF92_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBI9793487.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            6783..>7702
                     /locus_tag="LAF92_004591"
     CDS             6783..>7702
                     /locus_tag="LAF92_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBI9793488.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCN"
BASE COUNT         1892 a         2066 c         1810 g         1934 t
ORIGIN      
        1 cgctgaaaat tgttgataac gatattcgct acaaagccga cagtttcatc ttcaacgtcg
       61 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
      121 gctggtccaa cgctcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
      181 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
      241 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagta accaccacca
      301 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
      361 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
      421 tggaaattaa agttgtggaa cgtgaagggt aattcctgac tgatgtgtag gtcgcataag
      481 gtgcgaccta cacaccttct ccggcctaca aaacatcatt gagaaacaga aaaaaacagc
      541 gcccaacact tccccggacg ctgcatggca actttaataa tctcgcctta tcagaaagta
      601 attacaccct taatcaactc acgattgtta atcacatcgc gctcgtaggt ttctgccagc
      661 gtggcgaacg gatagcgatg ggttaacatc atgtcggcag tgattttccc ttctgccatc
      721 agtcgaccga ctttagcgaa atcttccggc gtggcgttgc ggctgcccat catcgtcgtt
      781 tcttttttat ggaattccgg atcggaaaac tgcaactcac ctttaaacag gccgacaaat
      841 accaccgtgc cgccgtgacg aatcagattc acggtgttat tcatcgcatg ttgattacct
      901 gtcgcgtcga tcactttctg cgccagcgaa ccaccaaact gcgcccgtag ctgcgcgtca
      961 aaatcttcgg ctgacgggtc cagtacaggt aattccagac gcgttgccac atgttcacgg
     1021 cgcgctggac tggtatccgc caccaccacc tgtgcgccat cggctttagc gattgccgcc
     1081 gcgcccagac cgattggccc cgccccgacc accagcacct gctcgccggg agcaatggct
     1141 gcgcgacgca ccgcatgagc gctaatagcg aaaggttcaa tcaatgctgc cgcctgcggg
     1201 tcaataccgt ctgcgggcaa aatgttcgcc accggcacgc tcaaatactc actaaaaccg
     1261 ccatcctgat gcacgccaat gactgaaatt ttttcacagc aattggtacg cccgctttta
     1321 cacgccgggc attgctgaca ggcaacataa gggatcacag caacttgctg accattttta
     1381 agatcagcaa tatttttacc cagcccaaca atctccccac atatttcatg gcctaaaaca
     1441 cgtggataac taaaaaatgg ttgatttcca ccccaggcat gaatatcggt accgcaaatc
     1501 ccgacagact taatttttat taatgcttca ttgtcacccg gaataggtat ctcgcgttgt
     1561 ttccagacta attctttcgg ctgctggcaa attaaaacat tcatcgtaga catgattcgg
     1621 ctctccattt tttgttgcct tagatatcaa tgaaaatgaa aaatggcagc ggaacggaaa
     1681 atctgttttg tgaaaacaca cgcataaaaa tgttttaaat cgggttttaa tggactcaca
     1741 aggaggaaaa tatgagtcgt tcacaaaatt tacgccacaa tgtgattaac caggtcattg
     1801 atgatatggc gcggggtcat atcccttccc cacttccatc gcaaagtgcg ctggcggaaa
     1861 tgtacaacat cagccgcaca acagtgcgtc atattctcag ccacttacgc gaatgcggcg
     1921 tcttaacgca ggttggaaac gactatgtta ttgcgcgtaa acctgaccat gatgacggtt
     1981 tcgcctgtac taccgcttca atgagtgaac aaaacaaagt gtttgagcag gcttttttca
     2041 cgatgataaa ccagcgtcag ttacgccctg gggaaacttt ttctgaactg caactggctc
     2101 gggccgcggg tgtcagcccg gtggttgtaa gagaatatct tttaaaattc ggccgttata
     2161 atcttattca aagcgaaaaa cgcggccagt ggagcatgaa gcaattcgat caatcctacg
     2221 ccgagcagct gtttgagcta cgcgaaatgc tcgaaacaca ttcacttcag cactttctca
     2281 acctgcctga tcatgatccc cgctggctgc aggcgaaaac catgctcgaa cgtcatcggc
     2341 tactgcgtga caacatcggc aatagctttc gcatgttctc gcaacttgac agagattttc
     2401 actccttact gctttcagct gccgacaata tcttttttga tcaatcgctt gagatcatct
     2461 cagtcatctt tcattttcac tatcaatggg acgaaagcga tcttaaacag cgcaatatca
     2521 ttgccgtcga tgagcatatg accattctca gcgctctgat ttgccgcagt gaccttgatg
     2581 ccacccttgc gctccgtaac catttgaatt cagctaaaca atcaatgatt cgatccatta
     2641 atgagaacac gcgttatgct cattaagtac ggattaaaaa cagccaacta ttagcgggtg
     2701 cacggtttta tcaaaagata aataccgaga aattcccgct tacctatgct cacaatcagt
     2761 cgactaaaaa tttttttgtg agcggtagta aagtcctaaa actttaacct gccgttaatg
     2821 tcaaccttgc gtaattcaca attacgtaaa caattaaaat tataaataca ggagtccggc
     2881 gtggaaaaag aaaatatcac cctcgatccg cgttcttcat ttactccatc ttcgtcggca
     2941 gatattcccg tgccaccaga tggattagtt caacgcagta cccgaattaa acgcattcaa
     3001 accaccgcca tgttgttatt attttttgcg gcggtaatca attatctcga ccgcagttcg
     3061 ctgtcggtag caaatttaac gattcgtgaa gaattgggat taagtgccac cgaaatcggc
     3121 gctttgctct ccgtgttttc actcgcttac gggattgcgc aacttccttg cggcccacta
     3181 ttggatcgta aaggcccacg cctgatgctg ggactgggga tgttcttctg gtcactgttc
     3241 caggcaatgt ctggcatggt gcacaacttt acgcagttcg tgttggtgcg tatcggtatg
     3301 gggattggtg aagcgccgat gaacccatgc ggtgtaaaag tcattaacga ctggttcaac
     3361 atcaaagagc gcggacgccc gatgggcttc ttcaacgcag cttctaccat tggcgttgcc
     3421 gtaagcccac cgattctggc ggcgatgatg ctggtgatgg gctggcgcgg gatgtttatt
     3481 accattggtg tactggggat ttttctcgcc atcggctggt atatgctcta tcgcaaccgc
     3541 gagcacgtag aactgactgc cgttgaacaa gcttatctca atgcaggtag cgtcaatgcc
     3601 cgccgagatc cgctcagttt tgccgaatgg cgcagcctgt tccgtaaccg tacaatgtgg
     3661 ggaatgatgc tcggattcag tggcatcaac tacactgcgt ggctgtatct ggcctggctt
     3721 cctggttacc tgcaaacagc ctataaccag gatttaaaaa gcacagggtt gatggcggct
     3781 atccctttcc tgtttggggc tgccgggatg ctggtcaacg gttacgttac tgactggctg
     3841 gtcaaagggg gaatggctcc gattaaaagc cgtaagatct gcattattgc cgggatgttc
     3901 tgttctgccg cctttacgct ggtcgtacca caagcgacaa catccatgac ggcggttctg
     3961 ctgattggca tggcactgtt ctgtattcac tttgccggaa catcctgctg gggcttgatc
     4021 cacgtcgcag ttgcttctcg catgactgcg tcggtgggca gtatccagaa ctttgccagc
     4081 ttcatctgcg cctcttttgc gccgatcatt actggtttta ttgttgatac cacccactca
     4141 ttccgtctgg cactaatcat ctgcggttgc gtcaccgcag cgggggcact ggcgtacatc
     4201 ttcctggttc gtcagccgat caacgaccca cgtaaagatt aatctcctta tgcccgataa
     4261 cattttgctt atcgggcatt ttcatggcga ttaaaatgtt tcccagttct cctcgctatc
     4321 tgccacggcc attttacgcg gcgcagctgg cgtcacggtt tttaccacag ccgatgtttc
     4381 acgctgctgt tgctgaatcc ggaacactgc cactgcttcg gtcaggcgac tggcctgctc
     4441 ttccagcgcg gcggcggcgg cggcagattc ttccaccagc gcggcgttct gttgagttac
     4501 ccggtccatc tcagcaaccg ctaagccaac ctgatcgata ccacggctct gctcatcaga
     4561 agcagaagct atttcgccca taatgtccgt cacgcgggtc acagcactga caatctccgc
     4621 cattgtttcc ccggcgcttt cgaccagtgt agagccaaca tccactttcc ccaccgagtc
     4681 ttcaataagg cttttaattt cacgagccgc ctgagcgcta cgctgggcca gattacgcac
     4741 ttctcccgca accaccgcaa aaccgcgccc ttgctcaccc gcacgcgctg cttcaaccgc
     4801 cgcgttcaaa gccagaatat tggtctggaa ggcaatgccg tcaattacgc tgataatatc
     4861 ggcgattttc tgcgaactgg tggagatatc gcgcatagtc tgcaccacgt tatctaccac
     4921 tttgccgccc cgttgcgccg tttcagaagc acttaatgcc agatggctgg cctggcgcgc
     4981 attctcggcg ttctgtttca ccgttgcggt cagttgctcc atgctggctg ccgtctcttc
     5041 cagcgaagcg gcctgttgct cggtgcgcga agagagatcg ttattgccgg tagcgatttc
     5101 gctggcaccg ctatagatgg cattggcccc gttgcgcaca tcaccgacgg tacgcatcag
     5161 ctctccctgc atatggcgca aactctctgc cagttgcccc atctcattag agccatccac
     5221 ctcaatcggt ttcaccagat cgccgcctgc aatatgacgg atgctgtcaa tcaggcgatt
     5281 cattggcgct accagcgagg ctttaatacc gaaccagacg gcgaagatga ccgccagtac
     5341 gacgatcatc acgcccacca gaatccacat cgcctggctg taggaggcat tgttatcgct
     5401 gacggcgata tcatagagcc gatcgttttg ctccatgtaa gccacatact gcttctcgaa
     5461 accgtcttga tatccctggg tcggctgatc aaagaactcg ttgattttgc ctgcacctaa
     5521 cagttggatc agctccgcca gcgcattgtg ataaatatcg taattacgtt tgatctctgc
     5581 cgctgcggcg gtgctctgac gcgggtcacg cggcaacgct tcgtaatccg cccagttttt
     5641 ttccgcctgt ttcagcgaaa tactggcact ctgcatcagc tcagcaacgg ttgaaccgct
     5701 accaatatta ttctgatcca tcatgtagcg gatacccgcg cggttgaggg tgttacgcgt
     5761 ctgcaacaac gcgacccagc tgccattcag cgtggattgc tgctggcgaa tggtttgtaa
     5821 aacagtgaaa ttttctttgt cattctttaa ggcattaaag aacagaccgc ctgatgtcag
     5881 ttgtaaaagg ccaaaaacgg ccaaaaccag cagtaagctg gtcacgatct tgatacgttt
     5941 taacatggtt tctctttcct gtggaccgat gcaagatttt cggcctggaa agcaaaaact
     6001 ttatgaaaag gtgtaacccg ccatccatgg aaaaaccagg cgctggttat cagatactaa
     6061 aaagttattc attatcgccc gggatggctt cttgtcggga attgcccaga gcaataaatt
     6121 cctctaacag atgggttaac tgttgcattt tctcggcagt aaactgagct tcaatctgcc
     6181 gataggcttc ttcaatctgt gtctgagcgc ggttatacag cgcctgcccc tctttggtta
     6241 gcgagatata gagcttccgc tgatcattga tcggctttaa tcgcaacact aaaccgtcgc
     6301 gttccatccg cgtaaggatc ccggtcaggc ttgggcgcaa aatgcatgcg cgatacgcca
     6361 gatcgtgaaa atccattgat gggctttccg ccagaatacg cacgatgcgc cactgttgct
     6421 cagtcaaatt atgccgttta acgattgggc gaaaataact catcgccgct tctcgcgcct
     6481 ggagcaacgc aatggttaga gagtcgtgca ttatctttcc cctgaaaaat tcaaaatcat
     6541 taatatagaa acagttaatc atcacgttac cgggactgcg ctgcccctgc aattcttcat
     6601 tttatcaaaa ttgagcatgt tttgttaact tgatgatatt taatatttta ttatctttaa
     6661 atgtaaatag tttgttaatt agatcacatt tacatcactt acttttgcca aactacgcaa
     6721 tcgggaataa aagtaatcat taacatatta atgattaaaa tgatcgaatc cgaggagtgg
     6781 taatgaaagg cactatcttc gccgtagcgt tgaaccatcg cagccaactt gatgcatggc
     6841 aggaagcgtt ccagcaatcc ccctacaaag ccccgcctaa aactgcggtc tggtttatta
     6901 aaccgcgcaa tacggtgatt ggttgcggtg aaccgattcc ctttccacag ggtgaaaagg
     6961 tactgagcgg tgcgactgtt gcgctgattg tgggaaaaac ggcgacgaaa gtacgtgaag
     7021 aagatgcggc agagtacatc gccggatatg cgctggctaa cgacgtcagc ctgccggaag
     7081 agagctttta ccgcccggca atcaaagcaa aatgtcgtga tggattctgc cccattggcg
     7141 aaaccgtggc tctcagcaat gtcgataatc tgaccatcta taccgagatc aacgggcgtc
     7201 ctgccgatca ctggaatacc gccgatttac aacgtaacgc cgcgcagttg ctgagcgccc
     7261 tgagcgaatt tgccacgctg aatccaggcg acgccattct gctcggcacg ccacaggcgc
     7321 gcgtggaaat acagccaggc gatcgcgttc gtgttctcgc agaaggtttc ccgccgctgg
     7381 aaaatccggt agtggacgaa cgtgaagtga ccacgcgcaa gagcttccca acgcagccac
     7441 acccgcacgg cacgctgttt gccctcggcc tgaactacgc cgaccacgcc agcgaactgg
     7501 aatttaagcc accggaagaa ccgctggtgt tcctgaaagc gccaaatacc ctcactggcg
     7561 ataaccagac ctccgtgcgt ccaaacaata ttgaatacat gcactatgaa gcggagctgg
     7621 tggtagttat tggcaaacag gcgcgtaacg tcagcgaagc cgatgccatg gattatgtcg
     7681 cgggctacac cgtgtgtaac ga
//
LOCUS       DAEVZA010000185         7619 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEVZA010000185 DAEVZA010000000
VERSION     DAEVZA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.185"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..358
                     /gene="cas6e"
                     /locus_tag="LAF92_004592"
     CDS             <1..358
                     /gene="cas6e"
                     /locus_tag="LAF92_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBI9793489.1"
                     /translation="ICKAGKRHDLLMEAKRQVKGQAEGRDIWLHQQQAALDWLAAQGE
                     RSGFTLLDTSVDAYRQQQLRRENTRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKS
                     RAFGCGLMLIKPGAEA"
     gene            355..1275
                     /gene="cas1e"
                     /locus_tag="LAF92_004593"
     CDS             355..1275
                     /gene="cas1e"
                     /locus_tag="LAF92_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HBI9793490.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            1275..1568
                     /gene="cas2"
                     /locus_tag="LAF92_004594"
     CDS             1275..1568
                     /gene="cas2"
                     /locus_tag="LAF92_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HBI9793491.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   1665..2366
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1665..1692
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaacc"
     gene            complement(2448..3485)
                     /gene="iap"
                     /locus_tag="LAF92_004595"
     CDS             complement(2448..3485)
                     /gene="iap"
                     /locus_tag="LAF92_004595"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBI9793492.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            3737..4645
                     /gene="cysD"
                     /locus_tag="LAF92_004596"
     CDS             3737..4645
                     /gene="cysD"
                     /locus_tag="LAF92_004596"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBI9793493.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            4647..6074
                     /gene="cysN"
                     /locus_tag="LAF92_004597"
     CDS             4647..6074
                     /gene="cysN"
                     /locus_tag="LAF92_004597"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBI9793494.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            6074..6679
                     /gene="cysC"
                     /locus_tag="LAF92_004598"
     CDS             6074..6679
                     /gene="cysC"
                     /locus_tag="LAF92_004598"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBI9793495.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            6729..7052
                     /locus_tag="LAF92_004599"
     CDS             6729..7052
                     /locus_tag="LAF92_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBI9793496.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            7246..7557
                     /gene="ftsB"
                     /locus_tag="LAF92_004600"
     CDS             7246..7557
                     /gene="ftsB"
                     /locus_tag="LAF92_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBI9793497.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
BASE COUNT         1839 a         1881 c         2077 g         1822 t
ORIGIN      
        1 aatctgtaaa gcgggcaagc gccacgatct gctgatggag gcgaaacggc aggtgaaggg
       61 gcaggcggaa gggcgtgata tctggttgca tcaacaacag gcggcgctgg actggctggc
      121 agcgcagggg gaaagaagtg gttttacact actggatacc tcggttgatg cctaccgaca
      181 acaacaactc cggcgggaaa acacccggca actgatccag ttcagtagcg tggattatac
      241 ggggatgctt acggtcaccg atcccgggtt atttctgcaa cgtctcagtc aggggtatgg
      301 caaaagccgg gcgttcggct gtggtctgat gctgatcaaa cccggagcag aggcgtgacg
      361 tttgtaccac tgagcccgat cccattaaaa gatcgcacct ccatgatctt cctccagtac
      421 ggtcagatcg acgtactgga cggcgctttc gtgctgatcg acaaaacagg gatccgaacg
      481 catattccgg tgggatcggt cgcctgcatt atgctcgaac caggaacgag agtttcccac
      541 gcggcggtgc atctggccgc cacggtggga acactgctgg tctgggtcgg tgaagcgggc
      601 gttcgcgttt actcctccgg acaacccggt ggggcgcggg cagataaatt actctaccag
      661 gcaaagctgg ctttaacgga agatctacgg ttgaaggtgg tgcgcaaaat gtatgaatta
      721 cgttttcgtg aaccacagcc agctcgccgt tcagtggagc agttacgtgg aattgaggga
      781 tctcgcgttc gccagacgta tgcattactg gcgaaacaat atggtgtgaa atggaatggt
      841 cgcaaatacg atcctaaaga ctgggaaaaa ggcgatgttg tgaatcgctg catcagtgct
      901 gctacatcat gtctgtacgg tatatctgaa gcggcagtat tagccgcggg atatgcgccc
      961 gctattgggt ttatccatag tggcaaaccg ctttcatttg tttatgacat agccgatatc
     1021 attaaatttg attcggttgt gccaaaggca tttgaaatag cagcgaggca acccgcagaa
     1081 cctgataaag aagtcagatt agcctgtcgc gatattttcc gtagcactaa gttaacgggc
     1141 aaattaatac cgttaattga ggaagtcctt gccgcaggtg aaattgaacc gccacaacct
     1201 gcgccggata tgttaccgcc agccatcccc gaacctgaaa cgttgggcga tagcggtcat
     1261 cgaggacgcg gctgatgagc atggtcgtgg ttgttacaga aaatgtcccg ccgcgcttac
     1321 gtggacggct cgcaatctgg ctactggaag tgcgtgccgg tgtgtatgtt ggtgatacat
     1381 caaaacgtat tcgggagatg atctggcagc aaattaccca actggctggt tgcggaaatg
     1441 tggtgatggc ctgggcgacc aataccgagt cgggttttga atttcagacc tggggagaaa
     1501 acagacgtat tccggtggat ttggatgggt tacgtttggt ttcttttctt cctgttgata
     1561 atcaataagt tatccgttct ttaaaaataa ggaaatgttt taatttagtt ggtagattgt
     1621 tgatgcggaa taaatttgtt taaaaacagt tatgtatgct tagtgtgttc cccgcgccag
     1681 cggggataaa ccggtcacga ctgaaaaaaa acgcgtcacg cgcgcgtgtt ccccgcgcca
     1741 gcggggataa acctctaaaa tagaaattat tcacggggat gctaatgtgt tccccgcgcc
     1801 agcggggata aaccgtggca gatcgatttt accgccagaa tgacagggtg ttccccgcgc
     1861 cagcggggat aaaccgatta aggcggacga ggaaaaggca gacgttacgt gttccccgcg
     1921 ccagcgggga taaaccgtta ggcacagaaa aaaggctact cagcgaactg tgttccccgc
     1981 gccagcgggg ataaaccgat caggtaatcg agacacctgt cagcactctc gtgttccccg
     2041 cgccagcggg gataaacccc cagcgccaga cgggaacaca attttaaagc cgtgttcccc
     2101 gcgccagcgg ggataaaccg ttgtcgggga tctgttcaaa ctgactcatt aggtgttccc
     2161 cgcgccagcg gggataaacc gggggcgcat taacagagag aaaaacgcat aggccgtgtt
     2221 ccccgcgcca gcggggataa accgcagctc gcagcgctcg gaacgtggcg ctataggtgt
     2281 tccccgcgcc agcggggata aaccgcggct atggaattta tggagaagtt tggttttgtg
     2341 ttccccgcgt cagcggggat aaaccgggcg cactggatgc gatgatggat atcacttaga
     2401 attccccgcc cctgcggtag aactcccagc tcccattttc aaacccatca agacgccttc
     2461 gccagctcct tcaccaacgg tagcattatc cgcataacgt cacggcagcg acgttctatt
     2521 cttccaggaa gtgccttatc aatatgttgt tgattatcca gtcttacgtc atgccagcta
     2581 tttcccgccg ggaaggcagg tgtttttgcg cgttgctgat aaccatcctt attcccaaga
     2641 ttccagttag tcgcttccac cgaaagtaca gcaatgcccg ctttgtcgaa tatttctgcg
     2701 tcattacaac acccagtgcc tttcggataa tttttattca aacccgggtt ggtcgttgcg
     2761 gctattccgt gactgcgcgc aattgccagc gccctgtcgc gcgttaattt ccttactgct
     2821 tcaggagttt ttacaccgct gttgaaatac aatttatcgc caacaattaa gttatcgaga
     2881 ttaatcacca gcagcgtatt tttcttttcg gtgtcactca tccgcttgag taaattctca
     2941 gcgcctaatt tcccttcctc ttcgccgctg gtcgccacaa atcgaatacc atactcggta
     3001 ggcgtatttt tcaggcgttc tgccaattcc agcatgacac ctaaacctgc ggcgttatca
     3061 tccattcctt gtaacgtcag cccgccgaga ttggcatcgg cgtcagcatc gctcagcggg
     3121 gcgtaagtat ccagatgcgc cataatgatg atctgctgcg gcgctttgcc ttcatgagcg
     3181 gcaatcaccg tacttcccgt cacgttatgc cagctcttgc gattatcgcg ggcggtataa
     3241 atataccgac tattaaatgt ccgaatatca ctgcgataac ccatttgctg aaactgttgg
     3301 cgaatataat cggcagataa catttctgca ggagttccgg tcatgcgtcc cggaaagaaa
     3361 gtagcaatat gtcgtgcctg agtattagca aaatcgccag gtttaggtga cgaggcgtgt
     3421 acggggagaa taaagcatac gccgagcgcc agggcagcgg tacggtggcg caatgcggaa
     3481 aacatagtga gtccttaaat accatgcaaa tttttttacc gccatagtat gaaactgccg
     3541 ctgcgctaaa acaatttcaa atcttcctaa acgcccgaaa tccggtgcct taagcacttt
     3601 ttgatattag ctttgccaaa tcgttattcc gttaaggaac tactcattct aattggtaat
     3661 ttcattcgtt ctcttacgct ccctatagtc gaaacatctg atggcaagaa aatagcggta
     3721 ttgcaaagga acggttatgg atcaaatacg acttactcac ctgcggcaac tggaggcgga
     3781 aagcatccac attattcgcg aggtggcggc agaattctca aatccggtga tgctctactc
     3841 tatcggtaaa gattccagcg tcatgctgca tctggcgcgc aaggcgtttt atccaggtac
     3901 gctgcctttc ccgttgctgc atgtcgatac cggctggaaa ttccgcgaga tgtatgagtt
     3961 ccgcgatcgt actgctaaag cctacggctg cgaactgctg gtgcataaaa acccggaagg
     4021 cgtggcgatg gggattaatc cattcgtgca cggcagcgcg aaacataccg atattatgaa
     4081 aactgaaggc ctgaaacagg cgctgaacaa atacggtttt gatgccgcct tcggtggtgc
     4141 gcgccgtgac gaagagaaat cccgcgctaa agagcgaatt tactctttcc gtgaccgctt
     4201 ccatcgctgg gatccgaaaa atcagcgccc ggagctgtgg cacaactaca acgggcaaat
     4261 taacaaaggc gaaagcatcc gcgtcttccc gctctctaac tggaccgagc aggatatctg
     4321 gcaatacatc tggctggaaa atatcgacat tgttccgcta tatctcgctg cggaacgtcc
     4381 ggttctggaa cgcgacggta tgttgatgat gattgatgac aaccgtatcg acctgcaacc
     4441 gggcgaagtg attaaaaaac ggatggtgcg tttccgtacg ctgggctgct ggccgctgac
     4501 cggtgcggtg gagtcaaatg cacaaacact gccggaaatc atcgaagaga tgctggtttc
     4561 caccaccagt gaacgtcagg gccgcgtgat tgaccgcgac caggcggggt ctatggagct
     4621 gaaaaaacgt caggggtatt tttaagatga acaccgcact tgcacaacaa atcgccaatg
     4681 aaggcggcgt cgaagcctgg atgattgcgc aacaacataa aagcctgctg cgttttctga
     4741 cctgtggtag cgtcgatgac ggcaaaagta ccctgattgg tcgcctgctg cacgataccc
     4801 gccaaatcta cgaagatcag ctctcatcgc tgcataacga cagtaagcgt cacggcaccc
     4861 agggcgaaaa actggatctg gctctgctgg tggacggcct gcaagctgag cgcgaacagg
     4921 gcatcactat tgatgtggcc taccgctatt tctctaccga gaagcgtaaa tttattatcg
     4981 ccgacacccc cgggcacgag cagtataccc gcaatatggc gaccggcgcc tcaacgtgtg
     5041 agctggcgat cttactgatc gatgcccgta aaggcgtgct cgatcaaacc cgtcgtcaca
     5101 gttttatctc cacgctgctg gggatcaaac atctggtcgt agcgatcaac aaaatggatc
     5161 tggtggatta cagtgaagag acattcaccc gcattcgtga agactacctg actttcgccg
     5221 aacagttacc tggtaatctg gatatccgct ttgtgccgct ctctgcactg gaaggcgaca
     5281 acgtggcttc gcaaagtgaa agtatgccgt ggtacagcgg tccgacactg ctcgaagtgc
     5341 tggaaaccgt ggagatccag cgagtggtgg atgctcagcc aatgcgcttc ccggtgcagt
     5401 acgttaatcg cccgaatctc gattttcgtg gctacgccgg aacgctggca tccggtcgcg
     5461 tggaagtcgg gcaacgtgtc aaagtgctgc cctctggtgt ggaatcaaac gtcgcgcgga
     5521 tcgtgacttt tgatggtgat cgcgaagaag cctttgccgg agaagcgatc accctggtgc
     5581 tgacggatga gatcgacatc agccgtggcg atctgctgct ggcggcagac gaagcgttac
     5641 cggcggtgca gagcgcgtcg gtggatgtgg tatggatggc ggaacagccg ctttctccag
     5701 ggcagagtta cgacatcaaa attgccggta agaagacgcg cgcgcgtgtt gatggcattc
     5761 gctatcaggt tgatattaat aaccttaccc agcgtgaagt tgaaaacctg ccactgaatg
     5821 ggatcggcct cgtggatctc acttttgacg agccgctggt gttagatcgt tatcaacaaa
     5881 atccggtgac gggtgggctg atttttatcg atcgcctgag caatgtgacc gtgggtgccg
     5941 gtatggtgca cgagccagtt agccaggcaa ctgctgcgcc atctgaattc agtgcattcg
     6001 aactggaatt gaatgctctg gttcgtcgcc actttccgca ctggggcgcg cgcgatttgc
     6061 tgggggataa ataatggcgc tgcatgacga aaacgtcgtc tggcatagcc atccggtcac
     6121 tgtgcaacaa cgcgagctac accacggtca tcgtggtgta gtgctgtggt ttaccggcct
     6181 ctccgggtcc ggtaaatcaa cggtcgccgg ggcgctggag gaggcgttac ataaactcgg
     6241 cgtcagtacg tatctgctgg atggcgataa tgttcgccac ggattatgca gcgatctcgg
     6301 ttttagcgat gccgatcgta aagagaatat ccgtcgcgtc ggtgaagtgg cgaatttgat
     6361 ggttgaagcc ggactggtag tgctgaccgc atttatctcg ccacaccgcg ccgaacgcca
     6421 gatggttcgc gaacgcgtag gagaagggcg ctttatcgaa gtgtttgtcg atacgccgct
     6481 ggcgatttgc gaagcccgcg atcccaaagg cttatataag aaagcgcgtg ccggtgaact
     6541 gcgcaacttt acgggaatag attccgttta cgaagcgcct gaatcggcag aaattcatct
     6601 caatggtgaa caattagtaa caaatttggt acagcaatta ttagatctgt tgagacagaa
     6661 cgatattatc agatcctgag acaccaccgg gtttccatgc ccggttcgtc aaagtaacag
     6721 gatcaaatat gcgtaatagc cataacatta cactaacaaa taacgacagc cttactgagg
     6781 atgaagaaac cacatggtca ctgcctggtg ccgtggtcgg ttttatctcc tggttatttg
     6841 cgctggcgat gccgatgttg atttatggct ctaacacgct gttcttcttt atctacacct
     6901 ggcctttctt tctggcgctg atgcccgtcg cggtagtggt ggggattgcg ctgcattcat
     6961 tgatggacgg aaagctacgc tacagtattg ttttcaccct ggtgacagta ggcattatgt
     7021 ttggcgcact gtttatgtgg ctactgggct aatttgtact ttcttgtctc ctctgttcat
     7081 aattcaaacc gtaactaata atgagattat gttctgcacg ccctgggtat acgtaacaat
     7141 ggacaaatgt ggtacatttg cccgcgttgt cgcggtatcc ccaacagagg atgtagagtc
     7201 gtcttcggat gcatgggatg atgatgccgt ttttcagggg gcaggatggg taaactaacg
     7261 ctgctgttgc tggctattct ggtctggcta cagtattcgc tgtggttcgg taagaacggt
     7321 atacatgact atacccgcgt caatgatgat gtggcggcac agcaagctac aaacgcgaaa
     7381 cttaaagcgc gaaacgatca actttttgcc gaaattgacg atctcaatgg cggccaggag
     7441 gcgctcgaag agcgtgcgcg taatgaactc agcatgacca ggccgggcga aactttttat
     7501 cgtctggtgc ctgacgcgtc gaagcgcgca cagtctgcgg ggcaaaacaa tcgataaatc
     7561 agcccgggaa ttaacatggc aaccactcat ttggatgttt gcgccgtggt tccggcggc
//
LOCUS       DAEVZA010000186         7478 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEVZA010000186 DAEVZA010000000
VERSION     DAEVZA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.186"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..499)
                     /locus_tag="LAF92_004601"
     CDS             complement(<1..499)
                     /locus_tag="LAF92_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBI9793498.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     SGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQ"
     gene            complement(486..878)
                     /gene="trbF"
                     /locus_tag="LAF92_004602"
     CDS             complement(486..878)
                     /gene="trbF"
                     /locus_tag="LAF92_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HBI9793499.1"
                     /translation="MTANKKNQEFKIRKIRRNIEYSFRDSDRYFDLFIVFLVAGIVLW
                     AVMHVIFDVCIDSWMADPKLLNFQYMWNVLMKVIPFTLWALAAGFLVTFFLSPMCELI
                     FGNIMIFLLKRRMRRENTLREGSNNASH"
     gene            complement(832..1206)
                     /gene="trbJ"
                     /locus_tag="LAF92_004603"
     CDS             complement(832..1206)
                     /gene="trbJ"
                     /locus_tag="LAF92_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HBI9793500.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWILVSEIYYSRMTGLNESS
                     VISEETYNDSKQKESGIQNQKN"
     gene            complement(1136..1681)
                     /gene="trbB"
                     /locus_tag="LAF92_004604"
     CDS             complement(1136..1681)
                     /gene="trbB"
                     /locus_tag="LAF92_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBI9793501.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(1668..1949)
                     /gene="traQ"
                     /locus_tag="LAF92_004605"
     CDS             complement(1668..1949)
                     /gene="traQ"
                     /locus_tag="LAF92_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001732192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBI9793502.1"
                     /translation="MHKYKFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLAE
                     LIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAEQQEGGHVSH"
     gene            complement(2065..2808)
                     /gene="traF"
                     /locus_tag="LAF92_004606"
     CDS             complement(2065..2808)
                     /gene="traF"
                     /locus_tag="LAF92_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBI9793503.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIARLAEHYGIMFFYRGQEPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(2801..3058)
                     /gene="trbE"
                     /locus_tag="LAF92_004607"
     CDS             complement(2801..3058)
                     /gene="trbE"
                     /locus_tag="LAF92_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HBI9793504.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTANGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKNDERNEDE"
     gene            complement(3085..4935)
                     /gene="traN"
                     /locus_tag="LAF92_004608"
     CDS             complement(3085..4935)
                     /gene="traN"
                     /locus_tag="LAF92_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBI9793505.1"
                     /translation="MKRLLPLLLGLAVSVAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEFCTRT
                     ARITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNSTFNVGLVNGQSGTFPLSGATGLQVTQGQVLTGSGCTANGNCLSGGNGDR
                     QVYEKLASGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLKGTECSEPGGTKT
                     GVMEGKPWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESKTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKSPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(4932..5354)
                     /locus_tag="LAF92_004609"
     CDS             complement(4932..5354)
                     /locus_tag="LAF92_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI9793506.1"
                     /translation="MALTKKQREKLRMKFGGRCAYCGCELPEKGWHADHVQAVLRKSE
                     QCMKAAEKRIFRLKSTGDVFRPEADCPENLVPACAPCNLLKTTYSLEMFRTQVSLQVE
                     RGRRSSVNFRTAERFGLIQVVNKPVVFWFEQYQRESEV"
     gene            complement(5380..5751)
                     /locus_tag="LAF92_004610"
     CDS             complement(5380..5751)
                     /locus_tag="LAF92_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793507.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLTWGWSVKGTFNSDFQLCFRLQPEGPHHWY
                     HRYCRRSLRADFRTLVRLVNP"
     gene            complement(5748..6386)
                     /gene="trbC"
                     /locus_tag="LAF92_004611"
     CDS             complement(5748..6386)
                     /gene="trbC"
                     /locus_tag="LAF92_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBI9793508.1"
                     /translation="MSRKIFELFALTAGLFISAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKATAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKSQ"
     gene            complement(6413..6934)
                     /locus_tag="LAF92_004612"
     CDS             complement(6413..6934)
                     /locus_tag="LAF92_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793509.1"
                     /translation="MGFFSFLRKISNKEKGVVIYEKQWFSLLEKVSAFDEVRQWAESN
                     GGAYYPLLECRRLTDSENVRKSEDILNRLEFIDRRFREFISIMSEHINNDKTKSMWER
                     ELSEYPPEKIYQVRREGEHLSLKNYGDDITLNRDTAVATVQITVSRKCITQEEMDELY
                     ALSERLKEKCAGN"
     gene            complement(6997..7305)
                     /locus_tag="LAF92_004613"
     CDS             complement(6997..7305)
                     /locus_tag="LAF92_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793510.1"
                     /translation="MEKKLSSKYIHMYMSISRVFIRLTGNVLKLFPFAMVICPVVWFY
                     LCPEHIISDIVNDWQDADTLEKILLVKLYLKTVLILTVVSVVVLYLSSDKFMPVKRRK
                     "
     gene            complement(7332..>7478)
                     /locus_tag="LAF92_004614"
     CDS             complement(7332..>7478)
                     /locus_tag="LAF92_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HBI9793511.1"
                     /translation="QMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRN
                     CVFL"
BASE COUNT         1846 a         2089 c         1649 g         1894 t
ORIGIN      
        1 cctgcgtccg ggggaaaagc ccgccaataa tcccctgcgc cgcctggcag gaactgaggt
       61 tcatactgtt aatgtcgctt gccattttct gcaggaagtc ttttgcggtt ttgatttccg
      121 gcaccgttgt ctgcagggca aggtcaaaaa agtaaccggc agcattgctc ataatctgct
      181 taacaaaacg ctgcagctgc tcgccattaa taaaactgaa cgaaccgagg taggcatcga
      241 tgccgccaca tccggcattg atatccggca gcgtcatgga aatcagctga acatttttga
      301 cctgagtacg ggcatacagg gagccgccgt aagcatagcc ggcagcctgc ccctgccaga
      361 cgcccgactg tgtggtattg gaggcgaagc ccagcttgtt aaagaactga ttcatgtcgc
      421 tgttcacatc cgctgacgct gcaggcgtca ccgtcagtaa ggcggcacaa agaataaaaa
      481 gaggcttaat gcgaagcatt attgctgccc tcccttaatg tgttctcccg gcgcatacgg
      541 cgtttcagca ggaatatcat gatatttccg aatattaatt cgcacattgg ggacagaaaa
      601 aatgtaacaa gaaaccctgc tgccagcgcc cataacgtaa acggaatgac tttcatcaga
      661 acattccaca tatactggaa attaagtaat ttcgggtcgg ccatccatga atcaatacag
      721 acatcgaaaa taacgtgcat aactgcccat aaaacaattc cggcaacaag gaaaacaata
      781 aacaaatcaa aatatctgtc agaatccctg aaactatact caatattccg tctaattttt
      841 ctgattttga attcctgatt ctttttgttt gctgtcatta taagtttcct ctgatattac
      901 tgatgattca ttcagaccgg tcatgcggct gtaatatatt tcagacacca aaatccatcc
      961 cacaattgcc cccacaaaaa acacaacaga aacagaaaag gtaagacaca gcgaaacgac
     1021 tatttctaca gggctggcgg gataaaaaag cccctgacgg ataaagggga tattcaggct
     1081 gacgattatt cccgtcgcga tgccggatat tccggcaatg agtaacacca cctgcttatt
     1141 tcgcaccttt ttttcctccg tacatctgca gaacggtatc catccgcgcc ataaaaccgg
     1201 cggcatccgt tgccccctgt aaaagcggta atgcctcaag cgtgttgacg ttgaccagga
     1261 aggtggtcgg tgtggccacc gggatattcg ggaagaaggt ctgcatcacg tccggtggca
     1321 ccggtaatgc ttccggaaag gcggtatccc cctgaccatc cagggtgtag ggaaaaacgg
     1381 aaaagccgta ctgctgtgcc aactgcttca gtaccgggtc aaactggtgg cagtaagggc
     1441 aatgcccctg cataaacagc accaccttcc agtcggccag gttaacctgc ctgccgttgc
     1501 tgaggcggaa ccagcgggga gccggctttt ctgtcctggc tgacgtgtct gctgccggtt
     1561 gtgcaggctg cgcggccata ccctgcggat tccagagtcg ttcaatttca tcccgggtgg
     1621 aggcctgcac acctgtggcc atcatcaaca aaatgaccgg cagtaattta gtgagagaca
     1681 tgtccgccct cctgctgctc tgccatcatg gcctgttgcc tggcttccag tgcctcacgc
     1741 tcttcccggc tgacacgggc aatccatgca tccagcacct ggtatgcgcc gaacagcaca
     1801 aggatggccg caatcaactc cgccagaaaa aaagccatcc agggtttgct gtaaacaaga
     1861 cgggcgacga tatgaaacca gacacccagg gaaaataccc acattcccgt aatatcaagc
     1921 cggggtaaag agaatttata tttatgcaca gtaaaactct cctgtcatac agagaattaa
     1981 ataagggact accggaataa atggtgaatt attaccaggt tcagaattaa tggaatttac
     2041 tgacgaacgg aaaatatccg taattcagaa gttcggttta aaatcttcag aaacgttcag
     2101 gaactgtttt gccaggtcgt cctgcgaaat aaaaccgtat gataacggac gaacactgcc
     2161 ctgtttcggg tcaaccagca tcatggccgg gaaatatttc acgccgaggc gctgcgcctg
     2221 cccctggtca gtccgggaat ccggcaacag cggattaatc acgccatcca cggaaacggg
     2281 aataacactc agaccatagg tatccctgaa gccattaatg acctgcgcca gctgcccgtc
     2341 gatgggttcc tgcccccggt aaaaaaacat aatgccgtaa tgctctgcca gtctggcaat
     2401 cgcctgtcgc tgctgcgcct ggtctgctgc cagctggttc cgtaccgtgc cgttgtaatg
     2461 gctgtactgc aggttatagt ccagttcggg atgtgccagc atggcctttt tagcactcat
     2521 ggtgaaaagc ccggcctgct gagtccagta attctgcagc cggaaatatt tcacaaaatt
     2581 atccacaccg ggatacagaa tggcttcgta cagcgcccgc tttgttgccg tctgcagcgc
     2641 ggccagtttc tgcataatat ccggctcctg acggggggct gcaggtacag gtttattttc
     2701 tttttccttc ggatttattt tctcgttata ccactgccag cctgcatctt tccctgacgc
     2761 cggaaaaata aagcagcaga gtaacagtgg cagtaatgct ttattcatct tcattccttt
     2821 catcattttt tctttcatca ggtttctgca ggtcagtcag ggcagaaaaa caaaaataca
     2881 gaataaacag gagcacaccg gagtaaagga taacgaaagc agcggcaagc atcccgtttg
     2941 ccgtggtttc ttttaccgta tcccacgacc agataattac cggggaaatg acaatcgctg
     3001 taatcaaaag ccggataaga aaatcaataa aacggttcga cgtgaatatg accttcatca
     3061 tttcccccgg ttgtttccca taccttactg ccctgcctgt ttcagctggt cagcaatctg
     3121 ctctttcact ttctgcgtca gaacgccact gtccgggatt ttctggttat tcatcaggtc
     3181 ttcgtagaag ttagtgaaat ccagtctgtc gaactgaatt ttctgcagct catcaaccgt
     3241 aatcccccga cagtcaggac ttttggcgct gccaaacccg atacgcaact gcccgttacg
     3301 cccctgctgc tggactatct gcgccagctt cgaatcaaac tggcagtagc tgcgcttttt
     3361 ctgcaggcag acgcccagca cctttttcga acagaactcg cccacactca ccgtcagttt
     3421 gttgtccttt gcctttccca gtgccttttc ttcactgttg cacttcgcca gccccacatc
     3481 ctgaccccag ccaccatcct tacagcagtt actgaagccg gctgcggcct tacggcagaa
     3541 tttcgcctcg cccgtgaaag ccctgacatc cacaccattc agggcggcaa catccttacc
     3601 tgcggcggca agcgcagcca gctgtgatac tgcctcacca aaatcactgc tttttccgct
     3661 ctgtgcctta tcgcattcac cgtcaaggca gaagacatca ccaccacaga ccatcacttt
     3721 tcctgatgtt tttgactcgc aggaatacgt ggcatactca tgcagacagg ttccctcatc
     3781 ggaataaaac gcgcactgac gtgaggccag cgtacaggcc ggattatcga catatttctg
     3841 acaggtgccg ttatctgcgg actgagtgac atatttatcc cggtatgccc agcatgcctg
     3901 tgtgatgctc cagggtttac cttccatcac tccggttttc gtaccacccg gctctgaaca
     3961 ctcagtacct ttcagcacgc catccgcttt attaaacgga caactctcca cccactccac
     4021 ccgtggaacc cattctttat cacggacctt catcctgagt ttcagcgtaa aggtggacgc
     4081 gccgctggct agtttctcat aaacctgtcg gtcaccgttc ccgccagaaa gacagttacc
     4141 gttagcagta cagccactgc cggtcagtac ctgcccctgt gtcacctgca aacctgtcgc
     4201 cccggacaag gggaaagtac ccgactgccc gtttaccagg ccaacattga acgttgaatt
     4261 catgaaggta taacgagaat tcaggaagaa aaaagcggcg tatacagaca atgatgcact
     4321 ttccactgta ccggttaccg gagccgtgac actgaacacc agtttcagcc cgttcatact
     4381 gaaccggaac tgtgagtgcg gtatcgtcac ctcacgatat tcatccgtgt atttccagtc
     4441 tccggtgatc ctggcagtcc gtgtgcagaa ctcctccacc atcgtgtccc gttcacaggt
     4501 atagtttgta aattcactgc ggtttatctg ctgtgcacta cactgctggc ctgtgtttcc
     4561 tacaatgctg tcagccctgt tcaccacatc cctgccggtc tgaatgaacg gtgcatccgg
     4621 tgacagaatg tctttcggct tgttcatgaa tgactctgtg atagtttttc cggtttcacc
     4681 ggtcgcccat tccgtggtgc cgtcattttt caggccgcca tccccgccag cagtcacacc
     4741 accgtaatat tttgtctcgt ccggattagc gttatagccg gggatactct cctgtggctt
     4801 aaagccctga atactgcctg taccctgccc ctgaatctga cgggcaaaat cagaacccgc
     4861 ccggtaatca ctgttgctgt cagcctgtgc cacactgact gccagcccca gcagcaatgg
     4921 cagaagacgt ttcatacctc actctccctc tgatattgct caaaccagaa cacgactggt
     4981 ttgttcacca cctgaatcag cccgaaccgt tctgcggtcc tgaaattcac gctgcttctt
     5041 cgcccccgtt caacctgcag ggacacctgc gttctgaaca tctccagcga atatgttgtt
     5101 ttcagcaggt tgcacggcgc acaggccggg acgaggtttt ccggacagtc ggcctccggt
     5161 ctgaaaacat caccggtgga tttcagcctg aaaatgcgct tttctgcagc cttcatgcac
     5221 tgctctgact tgcggagcac cgcctgaaca tggtcggcat gccatccttt ctccggcagt
     5281 tcacagccgc agtacgcaca acggccaccg aatttcatac gcagtttttc ccgctgtttc
     5341 tttgtcagcg ccatcaatgc ctccttaacg ggaacagatt tacggattca ctaatcggac
     5401 cagcgtcctg aagtctgcac gcagtgagcg gcgacagtac cggtgatacc agtgatgagg
     5461 gccttcaggc tgaagccgga agcaaagctg aaaatcactg ttaaaagtcc ccttcaccga
     5521 ccatccccat gtcagacgtg gtattgctga ttcagcatcc tgccgcagtc gctgctccag
     5581 aagggcagcc atttttaccg gacaggcaaa ggcgatataa ggcggcagct ttgtaacagg
     5641 gaaaccatgc ccctgacagg acgcatatgt acgaaatccg tgaacgttca tcgcggccac
     5701 cagtgcacgg atgtccgggt caatccggta atagcctgca ggtaaactca ttgcgactta
     5761 tccccggtgc cgtccagcag tccctgagcc acctgccggc agtctcctgt ggcggccact
     5821 ttctccagcg cctgtctgac gcgcagattt ccccggatga tgtcgtgccc ctggctgcag
     5881 aacaccacca gagccggcac gctgcgaatg ccgtactgtg aaaacagcgt cgggtcaatc
     5941 tgaacaccat cggtcgcacc gtctttcacc agagacagca ctgcttcagc cgtggctttc
     6001 aggtcgttgt tcaccatacc ccgcagtgtg gccggaatac cgtagtgccg ggtttctccc
     6061 agcatccgtt tcagcccctc ttcgggtatg gaaaacgaca caaaatacac ggcaccctgc
     6121 tgtggtttat ccttctgagc agcctgctgt ttacggacca gttcatcgag aaactggtta
     6181 tcggaatgct gaaggggatt cgccctgacc tgctgttctg cccatgcctg cagctgtgtg
     6241 tccggttttt cacgtaactg ctgactcagt gtttcctgct gcttcaggaa ctggcggttt
     6301 tcgggtgtat tcacattttc cgaagcgtgg gcagaaataa acagtcctgc tgtcagggca
     6361 aaaagttcaa atattttccg gctcatatca tctcccggca cagtgatata atttaattac
     6421 cggcgcactt ttctttcagt ctctcagaca atgcatataa ttcatccatt tcttcctgag
     6481 taatgcattt tctgctgaca gtaatctgca ccgtagccac ggcggtatct ctgttcagag
     6541 taatatcatc accataattt tttaatgaca gatgttctcc ctctctccgg acctggtata
     6601 ttttttccgg tggatattca gacaattccc tctcccacat ggatttagtt ttatcattat
     6661 taatatgttc agacataatg ctgatgaatt cccgaaatct tctgtcaata aactccagtc
     6721 ggttcagtat atcctctgat ttcctgacat tttcactgtc tgtaagccga cgacactcca
     6781 gcagaggata atatgctcca ccattacttt cagcccattg tctgacctca tcaaaagcac
     6841 tcaccttttc cagcagtgag aaccactgct tctcatagat aaccacccct ttttctttat
     6901 tacttatctt tcgtaaaaac gagaaaaatc ccataacgca cctttactta tattgtacac
     6961 atgcacacac agttatattt atgagaacca ccagactcat ttcctgcgtt taacaggcat
     7021 aaacttatct gacgaaagat acaagacaac aacgcttacc actgtcagaa taagaaccgt
     7081 cttcagatac agtttgacaa gcagtatttt ttccagtgta tctgcatcct gccagtcgtt
     7141 aacaatgtct gagataatat gttcagggca aagataaaac cacaccacag ggcatatcac
     7201 catagcaaaa ggaaacagtt ttaatacatt tcctgtcaaa cgaatgaaca ctcgggatat
     7261 actcatatac atatgaatgt atttactgct taatttcttt tccatttaaa atattcctct
     7321 ttttgtttaa gttacagaaa tacacagtta cgtttacgcc acatcaggta gccgaagttt
     7381 ttctttgtgt tgggcggatt ttttcccgtc tcccagcgca tcacactgcg cccgaacggg
     7441 tggcactgtc cgctgtccgg atacatattc accatctg
//
LOCUS       DAEVZA010000187         7440 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEVZA010000187 DAEVZA010000000
VERSION     DAEVZA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.187"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            49..672
                     /locus_tag="LAF92_004615"
     CDS             49..672
                     /locus_tag="LAF92_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HBI9793512.1"
                     /translation="MLKIIKQHKFAFLFTALLSTFFLNMFPPSAAGYELNTVLQIVAG
                     VNLLQRRYQVVSFIVLLIMFVTTHWLHPVNAMIQLYYGSYLVFFIILSVIVFRQVIFS
                     ARINTESICAALSGFLLIGYIGFFMFSAIENHMPGAFRGLSNDELQMMNELFYYSFIS
                     ILTVGYGDIVAVSWPARNATILVILTGYIYSLVFIARIVSGFSVSEK"
     gene            757..1008
                     /locus_tag="LAF92_004616"
                     /pseudo
     CDS             757..1008
                     /locus_tag="LAF92_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(1297..1614)
                     /locus_tag="LAF92_004617"
                     /pseudo
     CDS             complement(1297..1614)
                     /locus_tag="LAF92_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1617..1755
                     /locus_tag="LAF92_004618"
                     /pseudo
     CDS             1617..1755
                     /locus_tag="LAF92_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="PsiA; frameshifted; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            1987..2148
                     /locus_tag="LAF92_004619"
     CDS             1987..2148
                     /locus_tag="LAF92_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI9793513.1"
                     /translation="MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTA
                     TMAYESGGK"
     gene            2241..2405
                     /locus_tag="LAF92_004620"
     CDS             2241..2405
                     /locus_tag="LAF92_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793514.1"
                     /translation="MAPFSLLALYGHEQPDCLEGCFPPGTGMNVRLRRSFFSPAFSGY
                     GQSEITSASL"
     gene            2816..3022
                     /locus_tag="LAF92_004621"
     CDS             2816..3022
                     /locus_tag="LAF92_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI9793515.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPRQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            3047..3334
                     /locus_tag="LAF92_004622"
     CDS             3047..3334
                     /locus_tag="LAF92_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793516.1"
                     /translation="MSTQNIHVSTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            3453..4274
                     /locus_tag="LAF92_004623"
     CDS             3453..4274
                     /locus_tag="LAF92_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBI9793517.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRAQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(4570..5142)
                     /locus_tag="LAF92_004624"
     CDS             complement(4570..5142)
                     /locus_tag="LAF92_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBI9793518.1"
                     /translation="MLSLFVEWVKLFTDKVTRGGKMKKWMLSICLMFINEICHATDCF
                     DLAGRDYKIDPDLLRAISWKESRYRVNAVGINPVTGYGSGLMQVDSQHFNELARYGIK
                     PEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQRRLAYASDVY
                     RIYTGIKSSKGIRIPVTKKSLTEINSVQNN"
     gene            5512..5895
                     /locus_tag="LAF92_004625"
     CDS             5512..5895
                     /locus_tag="LAF92_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBI9793519.1"
                     /translation="MAKIQVYVNDHVSEKINAIAVQRRAEGAKEKDVSYSSIASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSTVAKILGIESLSPHVSGKPKFEY
                     ANMVEDIREKVSVEMERFFPKNDDE"
     gene            6086..6733
                     /gene="traJ"
                     /locus_tag="LAF92_004626"
     CDS             6086..6733
                     /gene="traJ"
                     /locus_tag="LAF92_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HBI9793520.1"
                     /translation="MCAMDRRERALISQLHFPHSLGEAISFLKYPVCVRFEDGSFIQE
                     NNRFEKLIRSSFNSCDEWFDSLTLECKLQLSRAEIESCSSVYGVNCNNDILLNNMLWS
                     VIIESVITPCGYFFIWRFIWVANDNLSSFVVSKYTNELIVLSDEYVGIEPYLIGFSHH
                     YSSAKMNITVSKSKKKTMKLFKRHGFSSRDLWLDEMIRTEKILPLYAKVKEILGR"
     gene            6869..7084
                     /gene="traY"
                     /locus_tag="LAF92_004627"
     CDS             6869..7084
                     /gene="traY"
                     /locus_tag="LAF92_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HBI9793521.1"
                     /translation="MSRGRTRAAPGNKVLLILDETTNQKLLAARDRSGRTKTNEVFIR
                     LKDHLNRFPDFYNASLVKEEAESIDDI"
     gene            7127..>7440
                     /gene="traA"
                     /locus_tag="LAF92_004628"
     CDS             7127..>7440
                     /gene="traA"
                     /locus_tag="LAF92_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBI9793522.1"
                     /translation="MDAVLSVQGVSAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AG"
BASE COUNT         1939 a         1570 c         1804 g         2127 t
ORIGIN      
        1 gggcattatc tgaacataaa acactatcaa taagttggag tcattaccat gttaaagata
       61 ataaaacaac acaaatttgc ttttcttttc acagcactgc tgagcacctt ctttctcaat
      121 atgtttccac cgagcgctgc agggtacgag ttgaataccg tgctgcagat tgtggcgggc
      181 gttaatctgt tgcagcgtcg ctatcaggtc gtttcattta ttgtgttgct gataatgttt
      241 gtcaccacac actggctgca tcccgtcaat gccatgatac agctgtacta cgggagttat
      301 ctggtctttt tcatcattct gtcagtgatt gtgttccgcc aggtgatttt ctccgcccgg
      361 ataaacacag agtccatctg cgctgcacta agtggctttc tgcttattgg ctatataggg
      421 tttttcatgt tttctgcgat agaaaatcat atgcctggcg cgttccgtgg actgtcaaat
      481 gatgagttgc agatgatgaa tgaacttttt tattattctt ttatcagtat tctgaccgta
      541 ggctatggcg atattgttgc cgtcagctgg ccggctcgta atgccactat tctggttatt
      601 ctcacgggct atatttactc actggtcttt attgcccgca tcgtcagtgg attttcggtc
      661 tcagaaaaat aactctttgt accagctacc agtcggctgt actacgggtt cctgagtccg
      721 gcaaaacggc atcgtgtatc gttccggtcg agtaggcagc ctaacccgac cactgaaaac
      781 gtcgcggcag agctgaagct gctgacggaa aattgctgcg atagtgggat ttctctggga
      841 ggtggatcgc cgcacgactg cgcgaaagga attgcgttgg tcgcaaccaa tggtggaaat
      901 attcgtgatt atgaaggtgt tgatcgttcc gcgaaaccgc tgctgccaat gattgcgatt
      961 aacaccactg cgggtacggc ttcggaaatg acccgtttct gtattatggg gtcagtttgg
     1021 atatcggaaa ttattgtact ataagggctt ccccggcatg tcaacgccgg ttttatagtt
     1081 tctgtctgct cgtcagtaaa ataaagggga gagactgcgg tactataaac ggccatgatg
     1141 aatcgttatc gattcgggcc ctgatgaaag acgcgcgtga ggttcttgtt cgttaagcgg
     1201 atacgtatat cctgtacaat agccagatag gcctcacggg gtctaaccct ttttcatcaa
     1261 cgagttgcag gtcagatggt tatactgctt tgtgctttag ctggctgagt acagcctgaa
     1321 atcatcaccg taatgcaacg atgcccagca cagcctcgcg tttttggcgg cgatggctac
     1381 aacagcccgc cagtaccccc tgcgctcaac caacgtacaa acccaacggc tgaatgagtc
     1441 agtccttttc tctgcaccaa tcaaaaccga acgggccccc tgaaccagaa gcgttcgcag
     1501 atatgaatcg cccgctttcg ttattctgcc aagctttgat tttccgccac tgctgtactg
     1561 tgatggtgta agtcccagcc aggctgccag ttggcgtcca ttcttaaaat catggcgttg
     1621 acaaggcaag tatggcagcc agagaggccg gtcacgctgt ccgtgagatg gagcggtgga
     1681 tggtgccgaa caaactccgg gaggcagcgt gatgcggcaa caatcacaca gattacccgt
     1741 aaacagtctg aatgagcggg ttattttcag gaaaagtgag tgtggtcagc gtgcagggat
     1801 atgggctatg atgtgcccgg cgcttgaggc tttctgcctc atgacgtgaa ggtggtttgt
     1861 taccgtgttg tgtggcagaa ggacaaaagc cccgtagtta atttttcatt aacccacgag
     1921 gcccctgcat gcttagacaa catcaggata gcctcttacc gcgctttgcg caaggagaag
     1981 aaggccatga aactaccggg aaacgccctt atctggtgcg tattaatcgt gtgctgcacg
     2041 ttgttgatat tcacactcct gacccggaat cgtctgtgcg aagtccggct gaaggacgga
     2101 tacagggaag ttacggcaac aatggcttac gaatccggcg gtaagtagca acccggaggc
     2161 gggcgcaggc ccgccttttc agggctgatg ctggtctgac tgcactgaag cgcctttata
     2221 aaggggctgc tggttcgccg gtggcccctt tctccttgct ggcattgtac gggcatgaac
     2281 aaccagactg cctggaaggc tgtttccctc cgggaacggg aatgaacgtg cgcctgcggc
     2341 gctcgttctt ctcccccgcc ttctctggtt atggccagtc agaaatcaca tcagcttccc
     2401 tgtgattatt ctctttttcc tcctgttctg actctgatta ctgctgccct gattactgtc
     2461 agttctgttg ttctcctgcc tgtgcctttc ccgggcgtcc ccctggcggg gcgggttgtc
     2521 gtgaacggag cctgcgaagc tgtctccgct ctggcgggct tccatccctg acgccaaacc
     2581 ccgggcttgc agcgaccgtt ccggtcgcgc cgcctctgtc gcgccgtggc ctgcgctcct
     2641 tacatcccgg caaaaggccg ccgggatgta aggcgcaacg ccgtttcgca aaccgcgctt
     2701 cacgtcgttg cggcgggtgc ctgcccgttg ttgtgtccgg tgccattcac ctgtttaagc
     2761 ccctgtcgcc gggggcaaag acagccacaa ccgtctccgc cagtccaggg gtgaaatgta
     2821 catcccgcaa aactttttgc ccaaaagcag gcaaaaactt ttgcggtctg cccccagaca
     2881 gtcgtttccg gccgtggcag tgatgcccct tgcggcgaag gttctcaaaa aggtgatggt
     2941 gaataccgga gggaacaaaa ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa
     3001 ctcaaaagat gatgggggct gaaaaccaga gagaggagaa agcaggatga gtacccaaaa
     3061 tatccacgtt agcaccgcgt cgtacacgct tcttgttgcc gggaagaaaa aaaacaccgg
     3121 ggaagagtgg gacgtcctag aattcagcag cctgactgag ctgaaaaaat atcgcaaaag
     3181 ccacccggaa aagatggcct tcagctatag ctacgcgctc agccggggtg tggatacgca
     3241 gttccgccat atcaacattg cggaagccga tcacttcaaa cagttcctgc gtcagattaa
     3301 gcgtgccggc ctcgatatcc gggcgatctg ctgaccggat gtgatgaata tccctcctga
     3361 ttgttgcaat cttgttaatg atggtaggga gggatgatat taagcggaaa aattttttat
     3421 taaatttatt ctttcataaa ggagtagctg ttatgcgatt agcttcccgt tttggtcggt
     3481 ataattccat ccgccgtgaa cgtcctttaa cggatgatga attaatgcag ttcgtgcctt
     3541 cggtattttc cggtgataaa catgagtccc ggagtgaacg ttatacgtat attccaacaa
     3601 tcaatatcat caataagtta cgtgatgaag gtttccagcc attctttgcc tgtcagagtc
     3661 gggttcgtga tttgggacgt cgcgaataca gtaaacatat gttacgtctt cgcagggaag
     3721 ggcatattaa cgggcaggaa gttcctgaaa ttatcctgct taattcacat gatggttcat
     3781 ccagttatca gatgatcccc ggaatttttc gttttgtctg cacaaatggc ctggtgtgcg
     3841 gaaataattt tggcgaaatc cgcgttccac ataaaggtga tattgtcggg caggttatcg
     3901 agggagcgta tgaagtgctc ggtgtctttg ataaggtcac tgataatatg gaggcgatga
     3961 aagaaattca tcttaacagt gacgagcaac atttatttgg tagagctgca ctgatggtca
     4021 ggtatgaaga tgaaaataaa acgccagtga cgcctgaaca aataattact ccccgtcgtc
     4081 gggaggataa acagaacgat ctctggacaa cctggcagcg ggctcaggag aatatgataa
     4141 aaggtggatt atcggggcga agtgcctccg ggaaaaatac caggacaaga gccattacag
     4201 gtattgatgg tgatataagg atcaacaagg cgttgtgggt gattgctgaa cagttcagaa
     4261 agtggaagtc atgataatat aagtaaagcc ccgaaaaatt ttcggggctt tacttatatt
     4321 atcattcttc ggcatcgctg tgtcgtctga tatacatcaa tttcatcaac tggctttccg
     4381 gttgtctgta aatattgtgc ccgcgaaaaa tggtctgaca ggaggctgcg cagttaccgg
     4441 gtatatataa tatgggttcc cgcttcgcgg gacttcggcc agtcagcatt atcactcata
     4501 tgatattttt gtgtgtggcc ttccatgccg cttttgcggc ataaccggta tctgacaatg
     4561 tctgtaagat taattattct gcacgctgtt aatttccgta agtgatttct ttgtgaccgg
     4621 aatccggata cctttactgc tttttatccc ggtataaatc cggtaaacat ctgatgcgta
     4681 ggcaagacgt ctctggttct ggcgttcggt tttcctgaat cctgcattat atgcaccaac
     4741 ggcctcccag gtgacgcccc attttttaaa tgctattgcc agataataag caccggtata
     4801 aatgttcatg cagggatctg ttgtcagatg ttccggctta attccgtagc gggccagttc
     4861 gttaaaatgc tgggaatcta cctgcatcag tccgctgcca tatcccgtta ccggattaat
     4921 accgacggca ttaacccggt aacgggattc tttccatgat attgctctca gtaaatcagg
     4981 atctattttg taatcccgtc ctgcaagatc aaagcaatca gtggcatggc agatctcatt
     5041 tataaacatc aggcagatgg ataacatcca ttttttcatt tttccacctc tggtgacttt
     5101 atccgtaaat aatttaaccc actccacaaa aaggctcaac atgttggtgg ttctcaccac
     5161 caaaagcacc acaccccacg caaaaacaag tttttgctga tttgtgtttt aaatcatgta
     5221 attaatgaac tatatttata ttatataatt ttgaattata ttgcagagat tcgtgataga
     5281 ttcgatagtg aatctatcac gaattgtcaa tgattcagta atgactattt tttttgaaaa
     5341 catctattta cttggcgcgt gaatctatat tagattcgtc atttgatgat tgctgattcg
     5401 tatagtgaat cgtgatttta gtatgatgaa tctaatggcg attcgtcata aggtgcagaa
     5461 tagagagcac gaaaatatat tgtttgttta ggaaggtgaa taaggtgtgt tatggccaaa
     5521 attcaggttt atgttaatga ccatgtgtct gaaaaaataa atgctattgc tgtgcaaagg
     5581 cgagcagagg gagcaaaaga gaaagatgtt agttattcaa gtattgcctc tatgttgctt
     5641 gagttaggat tgcgagttta tgaagcgcag atggaacgaa aagaatcagc gtttaatcag
     5701 gcagagttta ataaacttct tcttgagtgt gttgtaaaaa cgcagtcaac ggtggcaaag
     5761 attttaggta ttgagtctct tagccctcat gtttccggaa aaccgaagtt tgaatatgcc
     5821 aatatggttg aagatatcag agagaaggtg tcggttgaga tggaaaggtt ctttccaaaa
     5881 aatgatgatg aataaagtga aataaatttt gactttgttc aaatatcatg gttttttatg
     5941 gttcaaactg atgacaataa tcagaataat tagtatattg attatgtggt taatgccacg
     6001 ttaaaaattg aaactgaaaa tcgccgatgc agggagcagc cactccctgc atcgactgtc
     6061 catagaatcc ttagaggagg ttcctatgtg tgcgatggat cgtagagaaa gagcactcat
     6121 cagtcaattg catttccctc atagtcttgg agaggctatt tcgtttttaa aatatccagt
     6181 atgtgtccgc ttcgaggatg gttcttttat tcaggaaaat aaccgctttg aaaaacttat
     6241 cagatcgtca tttaattcat gtgatgaatg gtttgatagt ttaacgttgg aatgtaaatt
     6301 acagttatcc agagcagaga ttgaatcatg ctcttcagtt tatggtgtaa attgtaataa
     6361 tgacatatta cttaataata tgttatggag tgtgatcata gaaagtgtta tcacaccatg
     6421 cggctatttc tttatctgga gatttatttg ggtagcaaat gataatctgt catcgtttgt
     6481 tgtgtctaaa tacactaatg aattaattgt actatctgat gaatatgtag gcattgagcc
     6541 atatctgata gggtttagcc atcattattc atcagcaaag atgaacatca ctgtgtctaa
     6601 atcaaagaag aaaaccatga aattatttaa acgacatgga tttagttcta gagatctctg
     6661 gttggatgaa atgattagaa cggaaaagat acttccttta tatgctaaag taaaagaaat
     6721 attgggaaga taggctgtta acttattgtt actttgcggg aaccattggt tcccgttttt
     6781 gttaatatga tttactctat ttttgttttc attaaagatg atttatatga ttatatttta
     6841 ttatctcaag taattatgcg gaggaaacgt gagcagaggg agaaccagag ccgcaccggg
     6901 taacaaagtc ttattaatac tggatgagac aactaatcag aaacttttag cagctagaga
     6961 tcgtagcgga agaacaaaaa ctaatgaggt gttcattaga ctaaaagatc atctcaatcg
     7021 ttttccagac ttttataatg catctttagt aaaggaggaa gctgaaagca tagacgatat
     7081 ataaagagtt ttatttttca ctatcaatat taaacagagg aatcatatgg acgccgtttt
     7141 aagtgttcag ggtgtttctg cgcccgtcaa aaagaagtcg tttttttcta aattcactcg
     7201 tctgaatatg cttcgcctgg ctcgcgctgt gatcccggct gctgttctga tgatgttctt
     7261 cccgcagctg gcgatggccg ccggcagcag tggtcaggac ctgatggcaa gtggtaacac
     7321 cacggttaag gcgaccttcg gtaaggactc cagtgttgtt aaatgggttg ttctggctga
     7381 agttctggtc ggtgcggtca tgtacatgat gaccaaaaac gtcaagttcc tggccggttt
//
LOCUS       DAEVZA010000188         7322 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEVZA010000188 DAEVZA010000000
VERSION     DAEVZA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.188"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..332)
                     /gene="envY"
                     /locus_tag="LAF92_004629"
     CDS             complement(<1..332)
                     /gene="envY"
                     /locus_tag="LAF92_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBI9793523.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPS"
     gene            complement(515..1405)
                     /locus_tag="LAF92_004630"
     CDS             complement(515..1405)
                     /locus_tag="LAF92_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBI9793524.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(1406..4378)
                     /gene="nfrA"
                     /locus_tag="LAF92_004631"
     CDS             complement(1406..4378)
                     /gene="nfrA"
                     /locus_tag="LAF92_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBI9793525.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(4365..6602)
                     /gene="nrfB"
                     /locus_tag="LAF92_004632"
     CDS             complement(4365..6602)
                     /gene="nrfB"
                     /locus_tag="LAF92_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HBI9793526.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(6871..>7322)
                     /locus_tag="LAF92_004633"
     CDS             complement(6871..>7322)
                     /locus_tag="LAF92_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBI9793527.1"
                     /translation="HGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQ
                     KKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAE
                     LFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         1693 a         1915 c         1923 g         1791 t
ORIGIN      
        1 ttgctgggtt gctgcttgct gtggttggcg gctagccgga acacttccgg tgtacggcta
       61 tgacaagcga taaccggggt tgccagccgc gaccagggct ttacacaggt taagttctta
      121 tttaaaaaca acagataatc gttgatgatg ttacggttga tgtgggggat taatacgtga
      181 tttaacgttg agagttcaat gacgttattg ttgcacgcga aggcggccag gtagtttttc
      241 ggaagggtaa acgtagcatg gttattgacg cttacctcta cctctttttc ggtcaggatc
      301 accacgcaag gttcactgct gctcaattgc atttcgcact cctcagatat cagaaactcc
      361 gctcaaagga tctatgcttc ctgcatgagt gatcggcccg ttcgccgata acgatcttct
      421 ttctttagca cgctttttag caattaatct tgatggaatt ctgacgagag cgaaagaggt
      481 aagccaggtc gtacccgact tacctggagg agatttaata ctcgagaatg ccgtgcgcga
      541 cgggcaaata gcgcagagag aaatagagag tgtctttacc gcaagaggaa cgtttcgcta
      601 acagcgaggc gatctctgca tccggtttcg gtttcgctgt aaaggttttg cctttgccgg
      661 cgacaaacag ttcataaaca atgccgctgg caggggcggg actttggcaa tcggcgctgg
      721 cctgtagata acgttcacgc tgttcagcgg taagtttatc cacgccgctg ccatcctgta
      781 cccagacatt aacgccagtt gctttaactt gttccagcag ttggcgatag ccatcgggcg
      841 acatgtttcc ggcgaaaaaa ctactgatat aaaccggttt tgctgaaaca tcgctaatca
      901 gccgctgcgc gttgtttaac catgttagca aaggctgacg ggcagcttcg ctgcgccagt
      961 tcaggtcgtc aatttccgcg ctgatgtacc agccatccgg cgttacgcca ggcgcggcgc
     1021 tccataatct ggcttgctgg agatcggcag ccagcaggcg attaagatag ctttccagcg
     1081 ctgcggacga ctgtttctgg tgcataaaaa attccggatc ggcgttcagc ccgacaatca
     1141 gcttcaggcc agcctgttgc gcagctgcgg cacgcttaaa caataacgcg cgctgttctg
     1201 gctgggtgaa tgcatcgccg taacgggtcc attgcaaaac cagggtatcg aagccttgca
     1261 aacgtaactg actcatcagc ccctgccact gggtatcggt aacctgacta tctcggtttt
     1321 gtggttgcca gataatacct ttcatcgcaa aggaaaaagg gctgaccaaa agcagtgtca
     1381 gcaatacgaa aatgaactta cgcatttacc agtgcactcc aatggtgaga aacgcgttgt
     1441 tgcgctctcc gttacgttga ttaatcgcct taaaggtatg ttgatactcg acgccgagac
     1501 tgactttgtg cggccaggcg tcgtagtgcg tctcgccggt ccagatattc cagcggaccc
     1561 cgactccgcc aagctgcgcg ccctgagtgc ctttatcacg atagccgttg tcctgaacgt
     1621 gagcgtaagg ctcaatagtc tgtccgttag ctaccttctg atgccagctg acgcgataat
     1681 ctgccgtcca cgcctgaata tcctggcgga tatattgcgc cgcatcgagg tacaggtttt
     1741 gggcaaacca gcctgaaccg ttcgggtgcc attcgtcgct gtatttgccg ccattaaaga
     1801 atgaggcgct ggcgcgcagc atggtatcgg atgcgccatt ttgcccgttc agcggcaact
     1861 gctgttcgac ggcgaggaaa aagatctgat cgcgcagcgg cttccagcgc agaccggtgc
     1921 cggacatcgg atttttcacc ggcatcatca ccccgttttc tccggtatcg gcaaagacgc
     1981 ggctataaac tgagagcagg tcgccttcca gcagcatatt gcgtccgatg cggtactcgg
     2041 cttccagttg cccgtagcta cgatagcttt tccctggcgc tgcgccgccg acattattgt
     2101 tagcggtgct cattgccccg gaacgcaagc cgatagaaga atcgaaactg aacgtccagc
     2161 ggcgaccgac ctcctcatgc aaacggcgga aattgaagcg ttgctgattt tgctctgggg
     2221 tcagtggggt tatcagcgcc tgattatcaa tgtcatcaat caccagccgg gcgtaatgct
     2281 gcgtcgcagg catgtcatcc agacgctggt tcacataggc cagttgtcgg atcagtgccg
     2341 gatcgtccgg aagcccttta tgcgcctgtt cgagcatttc ccgcgactgt gcgatatcac
     2401 cgctatccca caaggcgtaa ccaagcgctg cctggatgtt gctattattc ggttccagtt
     2461 ccagcgcggc gcgcaaatca ctcaccgccg ccgggacatt atgacgttgg cgataaattg
     2521 tcgcccgcgc aacgtaagcg ttggcagaag gcgcaatatt gattgagcgc gtgagatcgc
     2581 tcagtgcgag ttccggctga ccaggaatgt aacgttgcgc atgcagccac cagtagaggg
     2641 cattgtttcc cagtccacgt tgttctgcct gttgtagcca gcgatcgcga gctgcaccat
     2701 ttcctgccgc ctgggcggta ttggcagcag caagcagatc ctcattgctc atgtcgtgaa
     2761 gactgatttt ctgccaggcc gccagtgcgg tggcgtagtc ctcaacctga tacgcctgat
     2821 aggctaccgc acgatgttgc caggcgttcg gttgtcgttg ttcggcctga agccatgcat
     2881 acagcgccac accgggtagc gtgtcccgat aacactttgc cagacggttc caggcggcag
     2941 catcgtagga aggcgacata tcgcccagca agcgaactat tgccgggcaa ttatctgcaa
     3001 tacccggcaa ctgactttgc cactgacgtt gctccgccag cggtaagggt ttcgataaaa
     3061 tcgccacctt cgccggcgtt gccaggtaag gatgactttc cagcagagac gccagtcgcg
     3121 ccattaaagt ctggctgaca cgcgcatcgc cctggaaagg atagcgttgc agcaataaat
     3181 cggcagcttc gcgtgactgc tcgttctgca tcagttgcca ggtcagttga tccaggcggg
     3241 taagatttgc cggttcttgc tgatacagca atcgtgccag acgcagagat tcagccttgt
     3301 tacgggtcgc cacgctgaca gcataacgct cctcaagcat ttcattggcg gggagggtgg
     3361 cgagcagttt ttgcgctgcg tcgtactgac cttcttttaa cagcaccggt agcgtcgcgc
     3421 caacaacata ctggcggtta tcggcaaact gcaccgtata attcgccaat gcctgaacgg
     3481 ggttggcgct gtatttagat aacagataga gccaactttt ctcttgtgcg tccgtggtaa
     3541 acagcggctt attttcattg agataatgct ggaggcgtgc tttttcgcca cgataagcca
     3601 gcgcggtcgc gtaagtaata tatgactgag gatcggtgaa gatcccctgt gattgcagtg
     3661 ccaggatccg atcgtccagc tgcccggcaa gaagcacgtc aaaccactga cggcgttctg
     3721 ccgcgcttaa tgtgttctgc tggcgtgctt cattgtatag cgtatctgcc tgggaccatt
     3781 gtttcaggta gattgcccgt tgcagcagat cggttcgcag cgtttttcct tctggcgatg
     3841 cagcaaacgt cgcatcgttc agttgcgctc tggcgacagg taattgtgcc agccgcaggg
     3901 cattctgccc gacttcactg cgacaacgca gggtcggcgc agcgtcgcac gctttttgct
     3961 gggcaagcag ttcttcaaca gtcgtaacgc ttttcacttc aaccggaata gccgccagac
     4021 tgcgctcaag tcgggcatct cctgggtgac gtttcagttg atcctcaagc aacagccgcg
     4081 cccggtcatc atgaccaaaa tggcgatagg cttccgcaag gtataaagtc agcggaatat
     4141 tatccggcac ctgctggtgt atatattcaa attcgcggat ggcggttgct tcgtcgttat
     4201 ttttctgtgc cttcagcgcc ttatcgagac ggggataaat aacaaaatgg cgataatcgc
     4261 tcagccccag ctcttctgcg ctggtgccga tattgtctgc gagtgcgctg gtactcaata
     4321 aagacgtcag cagtaaacca gaccatccga tgacgcgatt aaggttattc tccttcattt
     4381 tcggactcca gttgcgcaac ctgttctgtg tttaaacctg ctttgagtaa tagtgattgc
     4441 atcgaaactt gtaattcgcg ttgaattgtc aggacgcgat ccaacgtttc ctggctgata
     4501 acgccttcgg tgaccaaaaa cttgccgagc ggcagagaac tgcgttcatg gcgcaataac
     4561 aacacgttaa ttgctgaacg attaatatga ccgagcgtgg tcagtatttc ggcgaacagg
     4621 aactgatgcg gcacatattg ccgccagatt tcaccggtct gctgttccgt gagccactga
     4681 tgctgaaccg cattgtacaa cattgcccgc ggatcgtgac cgcgtcggcg tgcataccag
     4741 tgacgtaacc ccgtgacaat ttgtccccgc agaacaatga cgtaacgcac tttgcgtccg
     4801 actttacgcg tcagggccgc cagcgaaacc gggtcaatac catcttcact gccgacaatt
     4861 aactcatcat tttccagacg cagcggcagt actgcataat gcagcgctac ggaggctggc
     4921 atttcggcaa tcagcgagga cgggatctgc caggcatcga tggattccca cgccacgccg
     4981 ttttgctctg ccagcgcctg tgccagctgc tcggcgctaa tcagcccctg catcagcatt
     5041 gaaccgccca ggcgtagacc ttcgacgcga ttacgcagtg ctgtatcgag ttgttcttca
     5101 gtgatgacct gattttccag cagaatttgg cctaacgggc gcaacgagcg ggtatcgcca
     5161 gtcacgctgg ggaagtcatg cgttgtttta tcccacgcga cgcgacgtgg atcgccgtgt
     5221 tgaagtacct gttttagcgc gcgccagttg gccatgaagt taatcaggtt gccccagaaa
     5281 agacgcagga cagatagcag cccctgcgtc agaccgtagt agccagtgac gaaaatcacc
     5341 cgctgcacga tgcggttaac catcaagcca aagtttagcc acagcagggt cattaaccat
     5401 gcgctgccgc taaaaataga aaggaaatgc caggcattgg gccacaaact ttcatacgcc
     5461 agcaacagca aaagctggag catcaccagc atcgcgagga agctgacaaa gttactgatt
     5521 gcccctttgc ggtcgcgcca gagaaagtag ttcagcgtca ggctggaggt ccatttgtgg
     5581 gttttaaagc cctggaaaac aatgccgatg atccagcgag atttttgtcg aactgcagtc
     5641 gaaaaggtat cggggaaata ttcgcgcacg cagatcatgt ttgacgtccg tgcgtgctgt
     5701 aaaaatttgc gctgctcgcg ttctttggct tcgtccacca ccggaaaacg gacaaaaatt
     5761 tccgtcatac ctttctcttt caggcggaaa ccaatgtcgt aatcttcagt cagactctgc
     5821 acgtcgaaag caataccgtc accgtcagct aacagagcgg tcacggcgcg gcggctgaaa
     5881 caggtgccga cgcctgcgct gggcacttgt ccggcgaggg cttcacgcac cggaacatct
     5941 ttgccatgca gctctgaaaa ctcatcaatg taagtcatgc tggtgaagtg cgtccattcg
     6001 cgttcgaacg gatacaccgg gatctgaatc agatctttac gctcgaccag atagttgaac
     6061 agacgcaatt ccatcggtga aatcacatct tcggcgtcat gcagaataaa accagcaaaa
     6121 gcgaaattgg cgctacgttc aaactgggtg atggcgtcca gcacgttgtt cagacagtcg
     6181 gctttgctgg tgggaccagg acgcgcgcag actaccttat gcacgttcgg aaagcgagcg
     6241 cacacttcgt caacatcacg ttgagtatcg gggtcgttgg ggtaggtgcc aacaaagata
     6301 tgatagtttt cgtagtcgag cgtggtcgcc gccagctcgg ccatattgcc gatgacgccc
     6361 gtttcattcc acgccggaac cataatcgct aacggttttt catctggttt atacagttcg
     6421 cggtaactca ttcgcgggta gcggcgataa acactcaact tgcgtttaat gcggcgtacc
     6481 cagtagacga catcaataaa aaaatcgtcc agcccgctga tgaacatgat gaccgctaac
     6541 gttatcgcga ttacttttaa gccgtagagc caggttgcaa aaacatcaag aagccagtcc
     6601 acacaaaaac cttacattaa cgctggttat gtttagggtg gcgtatatta aggtttttta
     6661 tgaattgtga cagcttttta ccattaatag gtatgactat tgcggcacgt tatttttaca
     6721 ctggttataa aagttgccgt ttgcgtagaa tgtagacaaa agcatggtgt caaggagggg
     6781 aggttaacgt catagtgaaa aggagcggcg gcaccgagca ggtcataatg tagtggaagt
     6841 gcctggagga ggtgagcagg cagggcaaga ttacgaaagc ccgctccccg caaggactga
     6901 cgcgagatag tttctgtcca tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa
     6961 taccttatca ttcgttaaaa tattaatagc gatgtgccgt atccctgaaa ataattctgc
     7021 ggcgtttcct cttcttattt tgcagtcgtc ttcattcatt accacgtcca gacgccagtg
     7081 cagcttattc tccacgtgcc agtggtttcg gattgctgtg gcgaacttct ctgcggttaa
     7141 atcagcagaa ctgatataat atctgaccgt catttcgggc tctttctttt gttctgctat
     7201 tattgaccga aaggagactg ccacgcataa tttcttcaga cctttccatt caaacgtgaa
     7261 atcaataagt tcatcaggga catcgcaaac aatatgaaga cggatttctt ctctgccgtg
     7321 ac
//
LOCUS       DAEVZA010000189         7191 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEVZA010000189 DAEVZA010000000
VERSION     DAEVZA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.189"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(68..877)
                     /locus_tag="LAF92_004634"
     CDS             complement(68..877)
                     /locus_tag="LAF92_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBI9793528.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRS
                     SVYSSIDNLYKNGYIFLVDGETKEYEAKSPELILSQIEKSTIDNIKILKKELSQMMFQ
                     NEREFIYNVSGFDNLHQKAKEMLSSAECEIYLNTDFDLHFLKNEICEALNRHVRVIVF
                     SFNKLITPDPRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSNNRLL
                     INIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            complement(1284..2411)
                     /gene="ugpC"
                     /locus_tag="LAF92_004635"
     CDS             complement(1284..2411)
                     /gene="ugpC"
                     /locus_tag="LAF92_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBI9793529.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLKCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLYFNLDTSFCHIFDFYTEENLTNYL"
     gene            complement(2494..2982)
                     /locus_tag="LAF92_004636"
     CDS             complement(2494..2982)
                     /locus_tag="LAF92_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HBI9793530.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            complement(3073..4029)
                     /locus_tag="LAF92_004637"
     CDS             complement(3073..4029)
                     /locus_tag="LAF92_004637"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBI9793531.1"
                     /translation="MFSSLDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            complement(4048..5085)
                     /locus_tag="LAF92_004638"
     CDS             complement(4048..5085)
                     /locus_tag="LAF92_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBI9793532.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQNIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQ
                     DNIYVHAFYRF"
     gene            5358..6590
                     /locus_tag="LAF92_004639"
     CDS             5358..6590
                     /locus_tag="LAF92_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI9793533.1"
                     /translation="MKLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLVWSDATSV
                     EYMKYAAKEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            6593..>7191
                     /locus_tag="LAF92_004640"
     CDS             6593..>7191
                     /locus_tag="LAF92_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBI9793534.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIAAIIVGLFSTIYTL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAPNHIPPKNLVDWVGIKNFIMLFKFKIWSDTFLGVALWT"
BASE COUNT         2164 a         1384 c         1325 g         2318 t
ORIGIN      
        1 agtttagacg ttataaaaga caaatagatt gtgatcgcct acagacgatc acaatgaaag
       61 gaaaatatta acggtttttg ctatcactca aatgtttttc atcctgcacc atatcattgc
      121 cagattcatg caaagtattt attaatgcat tggcatctgg atattgttgc tgatacttgg
      181 ttagatatat atcactatgg atgtgttcgg caattatatt gataagcaag cgattattcg
      241 agtataaccc cagtgattca gctcgttgtg aaaaaaccat tactgtcttc atatcaacaa
      301 caagcataaa acggtgagaa gggtaatatt cttcttcatg gttaaagcgg taatacagtt
      361 ctacacgtgg gtcgggagta ataagtttat taaatgaaaa aacaataact cgaacatgtc
      421 gattcagagc ctcacaaatt tcatttttca aaaaatgtaa gtcaaaatcc gtgttgaggt
      481 aaatttcaca ctctgcagaa gaaagcatct ctttagcttt ctgatgtaag ttatcgaaac
      541 cagagacatt ataaatgaat tcacgttcat tctgaaacat catctgtgat aattcttttt
      601 ttagaatttt tatattgtca atagtgcttt tttcaatttg tgaaagtatg agttcaggag
      661 attttgcttc atattcttta gtttcaccat cgacaagaaa gatataaccg ttcttgtaaa
      721 gattatcaat agatgagtat acagacgaac gagaaagtga aatttctttt gctatcttgt
      781 aaccactgct acgaccgttt ttcaacagac tgatgtaaac ctgtgcatcg gtgcgagtga
      841 aaccaaaatt tatcaattta tttaccaaat gtatcataga cgcgctcctg aaatgaaaat
      901 gaaatcttac caattttaac attattaatt aacattttcg aaaaaattgc tgagctaaaa
      961 tgtggattta tcgtcattca tcctcaagct ggttacaggt ctacagtacg taagcattgt
     1021 gaactgcgaa tctgaatgcc ctccaactat ggtcagaaga catgctctga cctaaatagt
     1081 aggttcactg agtaagcggt gttatcaacg gtttattcat atatgctctt ttttatggat
     1141 ggaatttggt aactcaatta ccttgataag aatcagttcg tggagttaat gacatttacc
     1201 aatagacagt tcgagcagta agaagcctta tggaattgta aggtttaata ccgttattca
     1261 acctggggtt attgtcatat caatcacaaa taattagtta gattttcttc agtgtaaaaa
     1321 tcaaatatat gacagaagct tgtatcaagg ttgaaataca aatttttacc aatatcggct
     1381 gtagtaattg attgatcgtt gacttttgca ataaactctt tgttgttaac ggtaaagtaa
     1441 agatattttt cactgcccat attttcaata atacttaatg caccagtgac tgtattcatt
     1501 tctttgcact tcaaactcaa agagatatgt tctggacgaa taccaaaaca aacgggttta
     1561 tctatatacg ctttgagtac tgattgttta ttctcaggta tgtacaaccg aatatttttg
     1621 gcaatgtcaa tatagagttt accgtcttcc tcacgtacaa ccgcatctat caaattcatt
     1681 gacggggaac caataaagcc agcaacgaat ctgttagcag ggtggttata cagattaact
     1741 ggtgtgtcga cctgcattat tcgcccctga ttaagcacac aaatacgatc accgagggtc
     1801 aatgcttcaa tttgatcgtg tgtaacataa atcatggttg ctggatgacc ctcatccttg
     1861 agagactgat gcaactgagc aattttgact cgcattgaaa cacgaagttt cgcatcaagg
     1921 ttagatagtg gctcatcaaa taagaaaaca tctggcttac ggacaattgc acggcctacc
     1981 gcgacacgct gacgctgacc acccgacatt tctttcggtt ttcggtaaag taaatcgcta
     2041 atttcaagtt tttctgcaac ttcttcgaca attttcttaa tctcttcttt tggccttttt
     2101 tgcattttta aaccaaaagc catattgtca aatacagtct tatggggata cagcgcatag
     2161 ttctggaaca ccattgcaat gctgcgatct ttaggcggaa gggtattaac aaggcggtcg
     2221 ctaatgtaaa tttcaccacc tgatacgtct tcaagacctg caatcatgcg taatgtagtt
     2281 gatttggcac acccagatgg tccaacaaaa accatgaatt caccatcgtt tatatcgaga
     2341 tcaatgccgt gaacagcttt gaatccattg tcataaattt tttcaacttt gcgtagaatt
     2401 accgcggcca taatctgctc tcattaacaa gaattttgga atactgatac tccactattc
     2461 aacgaatgtt gaatagtgga gtgctaaaaa taactattta catggtgcta ccatggcggc
     2521 tattgccttt ttatcaggac caatttgaat cgtccaaaca tactttccat cggcaggaat
     2581 gacgactttt gtatttttat ccatgcctcc tttcttgagt gttttagcct gatttaatgg
     2641 caattcaaga cctgttacag tgaactgcgg gttccagttc atagtcgcaa attgcattgt
     2701 taccgcacct gctttttcag aataaataag ctgataaata ccatctccct tataatgcat
     2761 tttacgctct ggcttcatta accaatagcc ttccgggagg gttccggaaa tgtaaagacc
     2821 cgcagcaatt ggccctcttt catttacatc agggaggtta cattctgaat agcgaggatc
     2881 cgctttcacg acaccttcct gacttccaga gggtgaattg ccatcaacgc atcctgaaag
     2941 aagaccagtc aacacagccg caataagcaa tttatttttc atttaaactc tcaataaaag
     3001 tagtatagct tagttaagct atttctatgt cagtgttaat gttaatgtat ataaataata
     3061 cttctcaacg actcactgtt tatatacacc atgctgttta gttagtttat taaggacagc
     3121 gagatttttg ttgtattctc gcgtattttt ggcagggtaa ccaaaaaggt cactcttttc
     3181 aggacaagaa tggcttacac ctgatttagc cttaattacc gaataactac caattgatat
     3241 gtgaccggcg attccgactt gaccatgtgt aataacatgg tcgcctaaaa ctacgtgtcc
     3301 tgaaaaaccg cattgtgaaa caatcaggca tttattaccg attatgcagt catgcccaat
     3361 ctgcacttga ttatcaatac gagtgccttg gcctataatc gtatctccaa gtgtccctcg
     3421 gtcaatggtg ttgttacaac cgatttcaac atcatcacct atgattactc taccgatgct
     3481 atcaactcgg acatatgaat cacgttcatc cgacatatat tcaaaactat aattaccgat
     3541 ggagttatta gaatctatga taacgtcatt gccaataata gtaccttctt taatcactgt
     3601 attcgcatga atggcaacat tatcacctat ggtcacacag ttcataattt taacaccagg
     3661 cataaaatgg cagttcatac ctatctggca gtgtttacca atgtatacat caggtatcgt
     3721 tgaagtgttt tcttgctcaa acaaacgata cttatgtacc ttaaaatatc ttaaaacttt
     3781 gttaagatta ttcacgttca ggtaatcgac tacaatcttt gccttagcat tagaactaat
     3841 gatctcttcc ggtccgataa ttatatccgc accagtcgtt ggtatatgca acaaatctcc
     3901 aggtgtcata acaatcgaga gtccaccaac taaaggacta tggattggcc gaatggtatc
     3961 aaccctgagt gatggatcac catataaagt tccttgcatc aacgttttaa tatctagtga
     4021 gctgaacata ggtttccctc tgtttaatca aaagcgatag aaagcatgaa cataaatatt
     4081 atcctgattg agcacattat atttttcttc atctcttgga tcataaaagt ttttgtgctc
     4141 tttttcccag gcatgtttat attcaatctt ggctatccag tcgtcaccta tgttttgttc
     4201 ataacctaga gtggcttttc gaattgtttt gaaataggtt ctgtttgcat tgtattgcaa
     4261 actatctgcg ttttctgttt tcccgtgtcc gaagcgggtg cgtagataca gattacctgc
     4321 gttttcaaaa gcatatttga taattggctc gtagtaagtt tcggtataaa tactacgatc
     4381 atttatttta ccgtgattaa attcatcgtt atgctttttc tggtagaaaa attcaatacc
     4441 aaactcccat gcaccaaaac gtttataggg ttgaaatagt atactttgac cttcttcttc
     4501 gcgtacgccc caggagctct tatcatttcg actgtataag tattcaacgt ttgaataaaa
     4561 acctgcgttc cagtcagagt taaagtacgt caacgtaggg cgaaaactgt tgtcaacagt
     4621 tacagtatgc aagcctgtaa cataagggct atctatattt ccgtcggtat acatcaggtc
     4681 gtataccaga ctgaagtcga aaccgttacc aatagtgaac gttttgttta gtgatatata
     4741 gttttgcaga cttgtttcat tttcgtgata gcctttttct tcttgtcggc gatcgataga
     4801 cttaaggcca tagattgctg caaattgcca attaggttgg ctgaaatagg cctggaaaat
     4861 ctcgtgtgtt ttagtatatt cctttactgt tttacctaaa tagttatcag tgcttttggt
     4921 ctcatattca aatgtagtac caacatgtcc attaaagccc gcttgttctt ctgaccagtc
     4981 agtgtcggag gtaaagccgc gatcgtttgc cgcaaaggct cctgttgagc ctaaaaggac
     5041 tgttgcaatt gaagccgata agacattctt ttttattaaa accataaatg agttccttcc
     5101 aaaatatcaa tttaagatat gatttctgtt attccatata gtcgtacctc atgtactcct
     5161 aaacactctg cggcaataaa atattttttt cctcaacaaa ttactgcaaa tgggatatgc
     5221 atcactattt gcagttattc atcattaaaa tcttatttga cagttgattg gccttgattt
     5281 gtgttgataa tggcgtgaat gcttatgtgg tcgtacagca tgtactactt aaagatgtta
     5341 ttcatgaggt aaatataatg aagctttcaa atatcgtcac agttataatt cttgcaattt
     5401 ccagtacgct aacaccccag gcgatggcag aaaaattaat tcctgaaact gatgctgagt
     5461 tgttagtttg gtctgatgcc acaagcgtgg aatatatgaa atatgcagca aaggaattca
     5521 ataaagattt tggctacaag gtaaagttta catttcgcaa tatagcgcca atggatgcag
     5581 catcaagaat tatgcaggat gggggtacga ctcgtgtagc tgatgtagct gaaattgaac
     5641 atgataccct ggggcggtta gtcgttgctg gcggggttat ggaaaacatg gtctcagctg
     5701 agcggattaa aaaaacattt attccaggcg cagtatcggc agctacatat aataacatca
     5761 gctatggttt tcctgtaagt ttcgcaacgc tggcgctttt ctataataag gatttgttaa
     5821 acaccgcacc aaaaacattc gaagaaatca atactttcag tgaaaagttt aataattcat
     5881 ccgagcataa atatgctctg ctatgggatg tacaaaatta ttatgtttca cgtatgttta
     5941 ttaccttgta tggtgccaac gaattcggta aaaccggtaa cgatcctaaa gctctaggca
     6001 tcgcttcatc tgaagcgaag aaagggctag agacgatgaa acgcttaaag aaagcgaatc
     6061 cctctaatcc tcttgatatg ggtaatccac aagttctaag aggtctgttt aatgaaggta
     6121 aagttgctgc tgtaatcgac ggaccttggt ccatacaagg ttacattgac agcggaatca
     6181 attttggcgt gacacgcatc ccaacattag atggtcatca gcctcgcaca ttttcaacag
     6241 tacggctggc cgttgtaagc tcatttaccg aatatcctca tgccgctgaa ttattcgctg
     6301 actacctgac aacggataag atgttgatga agcgttatga aatgacgaat ctcatcccac
     6361 caatcgattc acttatgaac aaaattagcc agacaggtag tgaagctata aaagcaatta
     6421 ttgctcaagc taattattct gacgcaatgc catcaatacc ggaaatgtct tatctatggt
     6481 ctccaatgac taatgctatt ttggctacct gggttgagaa taaaacaccg gatgaagttt
     6541 taaatcatgc acaaacaatt attgaagaac aactttcgct tcaggagtaa ttgtgaatac
     6601 acaacaactg aaaatgaaga gtgcacctgt gctacctatt tcctgtttga ttatgggcgg
     6661 gactcagtta tcacgtcatt attatgtgaa aggcgggata ttctttgcaa tacaagtatg
     6721 ctttttgctt tatctttccg atattgtgca tacattaata ggtttgttca cattaggtga
     6781 tgtcgcgcaa attcgaaaag ggttgactgt tattcagggt gacaattcta tatttatgct
     6841 tgtagagggc gtgatagcgg ccattattgt tggcttattc tccactattt acacccttaa
     6901 tattaaagat gcgcggaact catcttattg ccatctgact tttaaacaac agttatataa
     6961 gctctatgag gataaatttg catttatagt cctaacgccc gcatttttag cgagtatagc
     7021 attcattgtt ttacctattg taattacagt tcttgtatcg tttacaaact atgctgcgcc
     7081 aaatcacatc ccaccaaaga acctggttga ttgggttggg attaaaaatt tcataatgtt
     7141 gtttaaattt aaaatatggt ctgatacgtt tctaggtgtc gctctctgga c
//
LOCUS       DAEVZA010000190         7064 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEVZA010000190 DAEVZA010000000
VERSION     DAEVZA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.190"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1854)
                     /locus_tag="LAF92_004641"
     CDS             complement(<1..1854)
                     /locus_tag="LAF92_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBI9793535.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNN"
     gene            complement(2061..2345)
                     /gene="ihfB"
                     /locus_tag="LAF92_004642"
     CDS             complement(2061..2345)
                     /gene="ihfB"
                     /locus_tag="LAF92_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBI9793536.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2505..4178)
                     /gene="rpsA"
                     /locus_tag="LAF92_004643"
     CDS             complement(2505..4178)
                     /gene="rpsA"
                     /locus_tag="LAF92_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBI9793537.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(4289..4972)
                     /gene="cmk"
                     /locus_tag="LAF92_004644"
     CDS             complement(4289..4972)
                     /gene="cmk"
                     /locus_tag="LAF92_004644"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBI9793538.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(5145..5909)
                     /gene="ycaL"
                     /locus_tag="LAF92_004645"
     CDS             complement(5145..5909)
                     /gene="ycaL"
                     /locus_tag="LAF92_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBI9793539.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(6078..>7064)
                     /gene="aroA"
                     /locus_tag="LAF92_004646"
     CDS             complement(6078..>7064)
                     /gene="aroA"
                     /locus_tag="LAF92_004646"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBI9793540.1"
                     /translation="RPLAAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYL
                     EQENYPPLRLQGGFTGGNVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYI
                     DITLNLMKTFGVEIENQHYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGT
                     VKVTGIGRNSMQGDIRFADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMT
                     IATAALFAKGTTTLRNIYNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLN
                     FAEIATYNDHRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         1779 a         1871 c         1657 g         1757 t
ORIGIN      
        1 gttatttcct ttatcgttgc tagcttgtaa aggccagtgc acgctgaaac gcaatccttg
       61 ccattgccac gcttcgccac gcacacaggg ctgatgatgt tcccagttta acggacttct
      121 gatccataac atcggccatg tgtgcaatat tgagtccagc cctccccggt gatccagatg
      181 ttcatgactc agaataacgc cttccggttc aagattatgc cagtggagcc aggggataat
      241 cagttgttgc ccactatccc cttcgggcca ggccagtcct gtgtcataga gaatcgcttt
      301 gccgtttctg gctatcacca ttgccagccc ttgcccgaca tcaagcatgt acacctgcca
      361 ctcgtcaggt cgaggttttt gccatagcgg ccagcacatc agcaagccta cagccacaca
      421 cattgctggc aacgttcgcc aggcgtttaa tcgccatacc accagtaaga accatgggga
      481 aaatgatagc cattgccaac gttcagcaat gttgatccac ccttctggca aactctttaa
      541 cccccagaaa agtaaagcca aagaccggtc ggcaagaaac cataaccctt gctccaggat
      601 taacggcccg cttaaatgca caaccatcgc ggcgaggatc aacggaaccg tgataaatgt
      661 caccaaggga attgctaata gatttgcaat aaacgaggtc agactaatgc catgaaatat
      721 gacgatttgc acgggcataa gcagaagtgt gatacccagt tgcagatgga tgagggaaac
      781 aactgcacgc aataccggcg gcagttgcca ctcaggacag ggaaaccact gataccaaaa
      841 aatcagtgcc gcgaccgcag cggcagagag ccataaactt tgcgagagaa tggcaacagg
      901 atccatcagc aaaattgccg ccagacaaca tatccataca tcccagccac tccactgtcg
      961 cccacttaac ttaagcattc cccacgtagc aagcgccacc acggtacgca atgcaggagg
     1021 ttgcattccc gtcaaccagg cataaaaagc agcacagcag atcccaccaa ttaatggtat
     1081 ttgccagtgg atccagcgcc caggcagaaa aatttgtcca ctgcgaatga gtccggcagc
     1141 cagcaacgcc gcaaaagcga tgtgcaatcc cgatatcgcc attaaatgcg ccgttccagt
     1201 atcgcgcatg atatttttga tttctttggg gacggataac cgttccccca tacctaaacc
     1261 aagaataacc gcattccacg gatagggttg cagcgttgtt tgtagtgacg ccagatactg
     1321 tgcacgcagg ctacaattcg gttcaataac acttgcctgc agaaagcggc cggtgagcgg
     1381 ttgatgctgg gcaatggcat aacgctggct atcaaagccg ccatcattaa gttggccatg
     1441 aaccgcacga actttgagtt tcattgacca ttgttgtcca gcacaaaccg cttgcggaag
     1501 atattcgcca tacagcacaa ggcctggcgc agggaatata cgtcgacctt gtagatgagt
     1561 aatttgaccg taatgagtgg tcatgccgtc agttgcagtg atctcaacaa ttgcatcctg
     1621 cgtcgcgcca gttaaggttt ctcctgccca caaaatttgc tttgctgata atatgcccca
     1681 aacgaaaaac agcaacgtca gcgcgatata acggacggtt ttaacaggga taaatgccag
     1741 tacgcaggcg aagagagtca gaaacgcaag ggttaatgtc ccaggcaatt ggggcaaaat
     1801 cagcaacgga aaaattccgc aaattatgca tacgccgact gtcgttattt tcatgcaccc
     1861 ctccctgttt atggaaagtg tgcaactttg ttgagcggtt gtctggtggg aattttgcgt
     1921 tccccgaggc acattcagaa gaatttaacg aaagggtgaa aactgctcaa aactctgcga
     1981 ggcagcttcc agttcctgat tagagaaatg aaaaaagcac ccgacaggtg cttttctcgc
     2041 gttcaagttt gagtaaaaac ttaaccgtaa atattggcgc gatcgcgcag ttctttacca
     2101 ggtttaaagt gaggaacgta ttttccttcc agttctactt tatcgccagt cttcggatta
     2161 cgtccggtac gtggtgcgcg gtagtgcaaa gagaaactgc cgaaaccgcg gatttcaata
     2221 cgctcgccct gcgcaagagt cgaggccata tgctccagca tctcttttac tgcatcttca
     2281 accgtcttgg cgggaatgtg cgattgctgg gtggcaagtc tttctatcaa ttctgacttg
     2341 gtcatgattc ctccggttcc ttaaaggcaa attaagcggc tgctgttgct gcattgatta
     2401 agggcggccg tagccgccct tagtgcttga ttacaggacg aaacctgcaa tctgtcaagt
     2461 aaactcaaca aacttcggaa taaaaatccc gaagagtcag agaattactc gcctttagct
     2521 gctttgaaag cttcagccat tgcgttgttg gagaagtttg catcttcctg tttgttaaca
     2581 gttgcgattg catctttctc gtcagcttcg tctttcgcac gaacagacag gctgattgcg
     2641 cggtttttac gatcaacgcc ggtgaattta gcttcaactt cgtcgccaac gctcagaacc
     2701 agggtagcgt cttcaacgcg gtcacgggat gcttcagaag cacgcaggta accttcaacg
     2761 ccgtcagcca gttctacggt tgcgccttta gcgtcaactg cagttacttt accggttacg
     2821 atagcgcctt tcttgttcag agcaacccag ttgttgaacg gatcttctgc gagctgttta
     2881 acgcccaggg agatacgttc acgttctgcg tcaacctgca gaacaactgc agcgatttcg
     2941 tcgccttttt tgtattcacg aactgcttct tcgcctgcaa cgttccagga gatgtcagac
     3001 aggtgaacca ggccgtcgat gccgccgtcc aggccgatga agataccgaa gtcagtgata
     3061 gacttgattt taccttcaac acggtcgccc ttgttgtggg tttccgcgaa ctgctgccac
     3121 gggttagctt tgcactgttt cagacccagg gagatacgac gacgttcttc gtcgatatcc
     3181 agaaccataa cttccactac atcgccaacg ttaacaactt tggacgggtg gatgtttttg
     3241 ttggtccaat ccatttcgga aacgtgtacc aggccttcaa cgccttcttc gatttcaacg
     3301 aagcagccgt agtcggtcag gttggtcacg cgaccagtca gtttggtacc ttccggataa
     3361 cgtttagcga tagctaccca cggatcttcg cccaactgtt tcaggcccag ggatacacgg
     3421 gtacgttcgc ggtcgaactt cagcacttta acagtgattt cgtcgcccac gttgacgatt
     3481 tcgctcggat gcttaacgcg tttccaggcc atgtcagtga tgtgcagcag gccgtcaacg
     3541 ccgcccagat caacgaatgc accgtagtca gtgaggttct taacgatacc tttaacttcc
     3601 atgccttcct gcaggttttc cagcagctga tcgcgctctg cgctgttttc ggattcgata
     3661 acggcacgac gagaaacaac aacgttgttg cgcttctgat ccagcttgat tactttaaat
     3721 tcaagctctt tgccttccag gtgcagagtg tcacgcaccg gacgaacgtc taccagagaa
     3781 cctggcagga acgcacgaat accgttcagc tcaacagtga agccgccctt aactttgccg
     3841 ttgataacac cggtaacagt ttcagcatct tcgtaagctt tttccagcgt gatccaggct
     3901 tcgtgacgtt tagctttctc acgggacagc agagtttcac cgaagccgtc ttctactgcg
     3961 tccagagcaa cgtcaacttc gtcacctacc tggatttcca gctcgccctg ggcgtttttg
     4021 aactgctcag ccgggatggc ggactcagat ttcagaccag cgtcaaccag tactacgtct
     4081 ttgtcgatag caacaacaac gccacgaacg atagaacccg ggcgggtttc gatttctttt
     4141 aaggactctt caaagagttg agcaaaagat tcagtcatgt ttaatcttca ggtttatatt
     4201 taacgtccac ctggctccat gccggatggg gttgtttaac atacccgctg tcattccatt
     4261 gcaacggggg tactgcaaat tcggtcgctt atgcgagagc caatttctgg cgcgcgtatt
     4321 gtagcgcttt ttcaatcact tgctcaatgc ttaaggtggt ggaatccaac actaaagcat
     4381 cggctgccgg aaccagtggc gctaccgctc ggttacgatc gcggtcgtcg cgttctttga
     4441 tctcggccaa aaggcgctca aagttaacac taaagccctt ctcctgcaac tgtagcatgc
     4501 ggcgatgcgc acgttcttcc gaggaggcgt caaggaaaat tttcactggc gcatcaggga
     4561 ataccaccgt tcccatgtcg cggccatcgg caatcaggcc tggtaattcg cgaaacgcgc
     4621 gttggcgacg caataatgct tcacgaacgc gtgggaatgc cgcgacttgt gaagctgcat
     4681 tcgccacttc ctgagtacga atttcgccgc tgacatcttc cccttcgagg atcacttcca
     4741 gattgccatt ggttgacaca aaacgtacat ccagatggga tgccagcggt accagcgcat
     4801 cttctgacgc aacatcaaca tggtgatgta atgccgccaa tgccagtacg cgataaattg
     4861 cacccgaatc cagcagatgc cattgcaatg cttccgccat agccttacac aaggtgcctt
     4921 tccctgcacc gcttgggcca tcaatggtaa taaccggggc aattgccgtc atctttatct
     4981 ccttaaaaca ggcgtaccgt taacataacc gcgcgcatta tacgcgccga cgccacaaat
     5041 cgttaacgtt gtgtgcaaaa accagttttg tctgtattca gatggggatt tattgcgcaa
     5101 ttatagcggg ctggcagaag accagcccga aagatgacaa tgatttactt accagaggcg
     5161 atacgatcac ggatgtgttg cgcacgctct gttgatggtg ggtgagagtc aaacatggac
     5221 tgggtgcgac cgccatccag actagccagt ttttcaaagc tgccaaccag cccctgggtg
     5281 ctgatgccac gtttcttcaa cagatcaaag gagaaatcat ctgcttcgga ctctttatca
     5341 cgggagtatt tagcattaat agcgccttct gcgatatcac ccaattgtga gcgggaaagc
     5401 tgggaagcca caccgctggt agctgaaatg gcatcgcgtg cggcaacgat cgcataagaa
     5461 gctttcattt cagccagcga gtgacccaac gcgacgtggc ccagttcatg gcccagaaca
     5521 ccttcaattt cgttgtcatt catcatgtcc atcaggccac tgtagacacg gacacagccg
     5581 ttcgccatcg cccatgcgtt gacgtcgctg gtcatataaa ccttatagtt gaccggcgtg
     5641 ccattaatat tgttacccaa tgctttggcg atttttgcca gacgtttacc gtacttgctg
     5701 gatttacttg cgacttgatt gccgctgtcc atttcggcac agccctgatt ggcaatcgct
     5761 tttgcatcgg catcgcttaa tgttgctgct ttataggcat ttaaaccgga gctgatagcc
     5821 atattggtat caataccgtg ggtattttga caccctgtca gtaatgctgc agaggtcgca
     5881 atcgccagca gtaatttagt attcttcatg tgaatcatcc gttgattaat catatataaa
     5941 tattatgtat caacaactta cattgacaag cgacgctacc attaatcaaa atgactcaca
     6001 aggtccgaaa aaaaacgccg acaatgtgcc gacgttttga taaagaaaga tttggctatt
     6061 tattgcccgt tgttcattca ggctgcctgg ctaatccgcg ccaactgctc gaaataatcc
     6121 ggaaatgttt tggccgtgca tttgggatca agaatcgtca ctggtgtatc tgacaacgcc
     6181 accagcgaga aacacatcgc catccggtga tcattgtatg tcgcgatctc ggcaaagttc
     6241 agtttttccg gaggagtgat acgaatgtaa tcgtgcccct cttccacttc cgcgccgact
     6301 ttacgcagtt ctgttgccat cgcaaacagg cgatcggtct ctttaacacg ccagttatag
     6361 atattgcgca gcgtggtggt gccttttgca aataacgccg ccgtggcaat ggtcatcgcc
     6421 gcatcaggaa tatggttcat atccatatca atagcgttca gttcaccacg cgtgcaggaa
     6481 atataatcat cgccccagca aatggtcgcg cccatttttt ccagcacatc agcaaagcga
     6541 atatcaccct gcatactgtt acgtccaata ccggtcactt ttacagtgcc gcctttgatt
     6601 gctgctgctg ccagaaagta agaagccgaa gatgcatcgc cttcgaccaa ataagtaccc
     6661 ggagactgat aagactgccc gccttttacg acaaattgtt gatagtgctg attttcaatt
     6721 tcaacaccaa acgtcttcat cagattgagt gtgatgtcga tataaggttt agaaaccaga
     6781 tcgcctttaa tacgaatcac cgtatcttcc ggcgcaagag gcgcagtcat taacagtgcg
     6841 gtgaggaatt ggctggaaac ggagccatca acgtcaacgt tgccgccagt aaagccgccc
     6901 tgtaaacgca acggcggata attttcttgt tccaggtaag tgatcttcgc cccgcccagg
     6961 cgcagcgcat ccaccagatg accaatcggg cgttctttca tacgcggctc accggtcagc
     7021 acaatatcat tgctacccag acaaagagct gccgccagcg gacg
//
LOCUS       DAEVZA010000191         7041 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEVZA010000191 DAEVZA010000000
VERSION     DAEVZA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.191"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..290)
                     /locus_tag="LAF92_004647"
     CDS             complement(<1..290)
                     /locus_tag="LAF92_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBI9793541.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAESW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRD"
     gene            complement(394..705)
                     /locus_tag="LAF92_004648"
     CDS             complement(394..705)
                     /locus_tag="LAF92_004648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793542.1"
                     /translation="MEIVIYITISILILDIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDAFPGMRLRHQVTGDTDDDRLAHSPADRMYTGKDHQKYTFCT
                     K"
     gene            complement(820..1029)
                     /locus_tag="LAF92_004649"
     CDS             complement(820..1029)
                     /locus_tag="LAF92_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793543.1"
                     /translation="MARRELELREIPYIKNSLHANYSYKSISIGSKQGWLISAKLKVP
                     ETFEPDMIFIEISDPEGFINIPDVL"
     gene            complement(1068..5930)
                     /locus_tag="LAF92_004650"
     CDS             complement(1068..5930)
                     /locus_tag="LAF92_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HBI9793544.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARQGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPGDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     IERAGTGAEGFRYDRHGNLLAWTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEIITPEGKTQYAYDKSGRLTGIFSPDGISQRTGYDERGRVNVTTQGRR
                     TIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWSGLVPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETERYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGELDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLLGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTG
                     FLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLY
                     EVYHEVIHAKQFAKIGREAYEALGRLSREEHVLNEILKSKNLFNEAEIAHAIKYVEGL
                     REKFMMGLTN"
     gene            complement(5950..6411)
                     /locus_tag="LAF92_004651"
     CDS             complement(5950..6411)
                     /locus_tag="LAF92_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HBI9793545.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(6439..>7041)
                     /locus_tag="LAF92_004652"
     CDS             complement(6439..>7041)
                     /locus_tag="LAF92_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793546.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
BASE COUNT         1406 a         2051 c         1797 g         1787 t
ORIGIN      
        1 cagtcacgca tccagttaat aaagcactca tgaaattttg caggactgat acaggataca
       61 actctggcaa tagtatcgtg aacaggaata ccattttcaa aatcaccata ttgcttcaag
      121 aaatcgagat gtgtttcccc gaaatcctct atatcttccc aactttctgc accagaaata
      181 acggcgcaaa tagtcaacag tagaatatcc gataatttat gttccacttt ccaggcttgt
      241 ctgtaatcgg gaataataga aatatgttcc atcaattttt taagttccat tttgttctcc
      301 ttaattatgt aaggagtatt tgatcatgta taagcaataa aaaacagctt caggtaatga
      361 ggaatatctc aatttttaaa cataaaatgt gaattattta gtacaaaaag tgtacttctg
      421 atgatctttc cctgtataca tccggtctgc ggggctgtgt gccaggcggt catcgtcggt
      481 atcgccggtc acctggtggc gcagacgcat tcccggaaag gcgtcataaa agaagcggca
      541 gctcagggca tcacggtcga acagcaccag gtaatcatcg cggcggcgca ggatgtactg
      601 ggcgtgatac gggttactgg tttcgatatt ggctaacatc cgctgtttta ttaatgcgat
      661 atctaaaatc aggatggaaa tagtgatgta tatcactatt tccatgatct tgccttgcgt
      721 tcatccaggc aggaatatcc ctgttagcag cctctgtgcg atgtacgcca gcggtctgta
      781 aagaaaccga ttaccttata acaaaacctc taacgtagtt tataatacat caggaatatt
      841 aataaaacct tctgggtcag atatctcaat aaatatcata tcaggctcaa aagtttcagg
      901 gacttttaat ttcgctgaaa ttaaccaacc ttgcttagaa ccaatactaa tagatttata
      961 gctgtaattg gcatgtagac tatttttaat ataaggaatt tcccttaact ctaactcacg
     1021 ccgagccaag tcaagagcca tttcgatagt tattttgtta tgttttatca atttgttagt
     1081 cccatcatga atttttctct caatccctca acatatttta tggcatgagc tatttccgct
     1141 tcattgaata aatttttact ttttaatatt tcatttagaa cgtgttcctc cctagataaa
     1201 cgtcctagtg cttcgtatgc ttctcgtcca attttcgcaa attgcttagc atgaatcact
     1261 tcatgataca cctcatataa ggtagcattt ttcctaagat aaatattgcg aaggccataa
     1321 tcaaaagctg ccctagcatt tggtggcaaa tatttatctg ctttccttat tactttaatt
     1381 cctagcttct ccatttcttt agcaatattg tttatttgac tacgtgataa acgaacccca
     1441 tccaggaaac ccgtatgccc actaacacga cgaccatcgc caatatagtc catcaaactg
     1501 ctgattttag aacttgcagc caatcccagc ggatcaaccc acccacaagg attcggcaca
     1561 taccggtaag tctgctcccc gcccagcaac cccagcggat cactcaccag gtacattccg
     1621 gtttccggct cgtaataccg gaaccggtta tagcacagcc ctgattccgc atcctgccac
     1681 tgtcctgcat acagcaggcc ggggtcggct tccgggtcca gttccccgcg cgggtccggg
     1741 tagccggtgt ctgcgggcag actgagtgcc agcccccaca ggctggtctg ccccggtcgc
     1801 cagacggttt taccttcact gttaaagagc atcagcgggc ggcccgtcag gtcgctcacc
     1861 gcgtgattcg tccgcgtcac ccactgcggg gccacggacg ggtgcggctg ccgtacccgg
     1921 ctgaactgct ggcttatcag ctcaaagccg ttaaacacca ggtgccttac gctgtacagt
     1981 ttatcatcgc ggtattcccg gatttccgca atactgtcgc catcccacag gtacgtaaaa
     2041 cggattttct tccggtcgca gcgtttttcc gtccgcctgc cgctggcgtc gtggcggtat
     2101 tcccactgct ccccctgtgc gctgcaatac ccggtcagct ggtcccggct gtcccaccgg
     2161 aaccgctctg tttccgggcg gtagccgtca cggtgttttg tccggctgac catccggcct
     2221 gcggcgtcat agtcatactg cgtgtttccg gcctgtttca gccggtggcc tccggcatac
     2281 cgctcagtct cttcacttat ccggtgcctg cccgcagact gcgccttcag gtagccacaa
     2341 ctgtcatagc cataggactc gtcgccttcg ccatagccca gcccggtgcc tgaggctgtc
     2401 accgccgtga cctggccgtt ccggtccagc cggtactccc gccgtgtttc ccgtccgtaa
     2461 tcttcccggc tggtgacagc cgacacatta cccgcgctgt cgtaacggta ctcccgtacc
     2521 agtgacggaa ccagcccgct ccatgtggct tcttccggaa aaaatccggc gcgctggctg
     2581 acaggctgac cggctgcatt ccagccctgt tcacaggcaa aaccccggtt actgtgccgg
     2641 agcacttcac gtcccgcctc atcacggctg aacgtcagcg tctcatcccc cggcaggata
     2701 acctccgtca gttctccggc ggcgttgtaa cggtaggtgg ttttcagcag ggattcatcg
     2761 ggatgtctgt cgcgttcatc ttccggtggc aggatacagc ggatgacggt gtgttcatcg
     2821 gggtagtggt attcaatggt ccgtcggccc tgagtggtga cattcacccg cccgcgttcg
     2881 tcatagccgg tgcgctgtga tatgccgtcc gggctgaaga tacccgtcag cctgccggat
     2941 ttgtcatacg catactgcgt tttgccttcc ggcgtgatga tttctgccag gcggcctgcg
     3001 gcatcccggc tgtagaggat ggtttcctcc aggccgttga ccctggcggt acagaggcca
     3061 tcggcgtcat agcggtaacg ccagaccacg ccgccccagt cacgctcttc cgtcacccgt
     3121 ccacagttgt ccagaatata acggtatgac tcgccctgcg gattgatgac ttctgtcagt
     3181 tgcagcgtgt ctttatcgta gcggtactgc cagcggtggc cttcaccgtc cgttcgcgcc
     3241 accggcaaat cgaacgggcc gtactccatt gtccagacaa caccattgcc gtccgtccag
     3301 gccagcaggt tgccgtggcg gtcatagcga aatccttccg cgccggttcc agcacgttca
     3361 atgccgctca gtttatggtg ctgctgccac tgtaaccgca ctgactgacc gccggggccg
     3421 cgcatcttca ccggcaggtc gtgaccgctg tattcggtgt ggtgttcacg gcccatcacg
     3481 tcggtgagtg tttccggccg gttgtggtgg tcgtactcta tgcccagacg ccgttcatcc
     3541 tggcggaata tccccgacag cagcccctga gcggtgtatg ccagccgggt ctcttcgccc
     3601 tgtggcgaca ccaggcttac cggcaggccg cgttcatcac actgctgtgt ccacgtctgc
     3661 ccggcagggt cggtgatgtt gctgagcagg ccgcgctcat catagcggta ctgccactcc
     3721 gcaccgtctg ctgccgtgta acggcttagc tggccgtgtg cggcgttgta ttcaaacttc
     3781 tccacccgcc cggcagggga gtggcgggcc agcaggtgat ggaattcatc ccattcatac
     3841 gtggtggtgc tgccgtccgg cgcttcttcc cgcagaatat tgccgtcagg atcatagtaa
     3901 taacggtgag tgcccttgcc gtccgtcacg gtggtgcagc cgtcaccata gtccagcgtg
     3961 gcgttgtaat agccctccgc cccggtgacg gtcacgcacc ggccctgttc atcgtaggtg
     4021 aaacggctcc acgtctggtc gttatcggac cagcggatga tccggtccgc agcgtcgtac
     4081 tcataaaaca ggtggtagtc cagccgcgca tccgcttctg tcagccgccc ccgcgcatcc
     4141 tgttcgtagg tcgccagcgt ctgtatgccg ttatccgtgc ggtgaatggc tttcaggtag
     4201 ccgctggcat ggtacgtcag tttaagccgg atcccgtcgg tgtgactgac acccgtgatc
     4261 cggtactggc tgtcgcgctc aaacgtgatg cggttgctgg cggtgtcact catcccgccc
     4321 agcatataca tccggtcacc ggggctgtgt gccaggcggt catcgctggt atcgtcagtc
     4381 accgggtggc gcagacgcat tcccggaaag gcgtcataaa agaagcggct gctcagtgca
     4441 tccctgtcga acagctccag gtaatcgtcg cggcggcgca gaatgtactg ggcgtgatac
     4501 gggttaaccg ccgtatcact gtcaccgtcg aaggcgaagt tcagcgtggc tccctggctg
     4561 ataatatcca cccggtttcc ctgttcacgc acccaggcgt attcgctcca ggagtcgctc
     4621 cagcccacgc cggtcaggcc atgcagcggc agtacagaac tgtggaaacg ggtgaaggag
     4681 agggggagtg tctggcccag ggtgaagtcg gtacgttcgt cgcagtacgc gccggttacc
     4741 ggatcgacag gatctgttac ccatctgatg actttattgg caatactttt taacgactta
     4801 cctgttttcc ccagcaacgc gccgagcttt gcccccagca gcttcccgct tttccccagc
     4861 aggttcccca gtttgccgag tctcgccaga ttcttcagtt ttcccagcag tcccgctggc
     4921 ggcagaagat agctggctat cgttattccc cccaggatca ttctctgcca cggcgggaac
     4981 tccggctgga tatccagata ggtcacctgc tcaccgccaa taaagacgtc cggcgaacct
     5041 cccacgattg ctgcgctgca ctccagtttg tcgccctggc gtgcggcagg ctgattgttg
     5101 ataaagacgt tgctgctgcc ctgggctatc ctcggtggtt tgttctcctt gtcgcagagc
     5161 gccgtgtcct caccggcttc cgggaaatcc acatcttttt tgatttcgcc acgcccgccc
     5221 accatggtgg caaactccat taataccgga ccggcgggaa acagaagcga tgcgccggcg
     5281 ctgacgcgat ccataaaacc ggcattgctg tccgtcaggg tgataacagc gttcccccag
     5341 gccttcgcca gtggtactgc tttttccgct gccgattcaa gcaggtcagt ggcaatatca
     5401 ccccatgagg gttccccctg tgggacttca ggtattgctc caggtggggg aggaggggga
     5461 agggtgacgg cggccctggc agcgggcttt ccgttaatgt ttacattttc agaaccggta
     5521 ttgatggcgc cgtccgtgtc acaaaagctg tttatccatg actcaagctg gttctgtttc
     5581 tcctccacat aatcactcgc cttatagctg gcatacatca gcgcaggcac cagaaccagc
     5641 gcataagggc cggtggcaca ggcggccata atcaggcaac cttctgcgat ggtcactatc
     5701 gcccccagca cggcccctgc cagcgccccc aaaaaactct tgtgctgaat ttcatcgccc
     5761 agcctggcgg cgggggggcc ggactttttt gatgccaaca tctgtgcaat ggctttgtcg
     5821 ttggctgccc gtttcatctg gctggcgacc atcgcctgct gtgacagcat tcgcattgcc
     5881 agcgtaccgc ctgcggtcgc tccctgtggc ccgcctggtc cttcactcat gtgttatccc
     5941 tcgtttatgt cagcgcggct ggtccttttg gatgtcattc tccggtggca acggggtgaa
     6001 gctctttatc acctccagca gcatcgtctt ttgactgttc ttcagggata aggaggtgct
     6061 gaaagtgaag gtcagcagcg tatctccacg ccgctggaat acggctatct ggctaatccg
     6121 cccctgattc ccctcatctt ccggaccgtt ccaggaataa tccagcagcc aggccggggc
     6181 ctctgccaga gtgatttccc ggcaggcatg ttcggtgtac ccatcaagtt tgccctggta
     6241 cagcgccagc gtttcctggt acagctctgg tgtcagggtt ccctgtggaa cggcttcgcg
     6301 ggtaatgacc aggttgatgc cactgttatc tgtggacacc aggacctgca ttgactgatc
     6361 ctgccattga gacggcagtt gaatgtggcc ttcattaaaa gtgaactgca taaaatactc
     6421 cctgataatc tgagtgaatc agttgatgaa aacagaaccg cccacgatgg tgatgccatc
     6481 cttcgacagc ctgattaccg tcgattcccc gacctgtagc tgaatgccat ccggcgtcag
     6541 cgcaattttg ctttcctcca cggccagcaa aatttcctta atcgccgtat aagcctgtat
     6601 accgtcgcct tcgccaccat tgtttacagc gtcaacatgc accgaaccgg caatcgtgcc
     6661 tctgtcctga aagacatcct cagataaaga ccctttcagt actccggtga tcttgtggcc
     6721 tttttccacc gtaagaaggc ggttaccttc ctggatcgtg accgtctggt cattttttac
     6781 ggtttcgcgg tgatcgtgtt ttacggtggt ggtacggtcg ttgagcactt cggtatccat
     6841 gttcttctgc gcgtggatgt agacctgttc tctccctgtc gcatcatcaa acttcagctc
     6901 attaaatccg ctgcccatgt acgttttgga gcggatggtc atctgcgtct ttgttcccgg
     6961 caggctgccg ggggtgcggt tttcctggtg gtaggtgcgc cccataatga tcggctggtc
     7021 cggatcgccg ttgaggaagt c
//
LOCUS       DAEVZA010000192         7010 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEVZA010000192 DAEVZA010000000
VERSION     DAEVZA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.192"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            267..446
                     /locus_tag="LAF92_004653"
     CDS             267..446
                     /locus_tag="LAF92_004653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793547.1"
                     /translation="MQQLVPPPGVKGIDKMVHYEVVQYLMDCCGITYNQAVQALRSND
                     WDLWQAEVAIRSNKM"
     gene            457..612
                     /locus_tag="LAF92_004654"
     CDS             457..612
                     /locus_tag="LAF92_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HBI9793548.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(609..1097)
                     /locus_tag="LAF92_004655"
     CDS             complement(609..1097)
                     /locus_tag="LAF92_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HBI9793549.1"
                     /translation="MLDVFTPLLKFFANEPLERLMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            1539..1760
                     /gene="kilR"
                     /locus_tag="LAF92_004656"
     CDS             1539..1760
                     /gene="kilR"
                     /locus_tag="LAF92_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HBI9793550.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            1760..1930
                     /locus_tag="LAF92_004657"
     CDS             1760..1930
                     /locus_tag="LAF92_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793551.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            2005..2280
                     /locus_tag="LAF92_004658"
     CDS             2005..2280
                     /locus_tag="LAF92_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793552.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPTEKKAEFLATLIAMKLKGEI"
     gene            2382..4982
                     /gene="recE"
                     /locus_tag="LAF92_004659"
     CDS             2382..4982
                     /gene="recE"
                     /locus_tag="LAF92_004659"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HBI9793553.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPVHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEQDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGISKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEQEEFSNRFIVAPEFNRRTNAGKEEE
                     KAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLSDCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            4975..5784
                     /gene="recT"
                     /locus_tag="LAF92_004660"
     CDS             4975..5784
                     /gene="recT"
                     /locus_tag="LAF92_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HBI9793554.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNSDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            5841..6035
                     /gene="ralR"
                     /locus_tag="LAF92_004661"
     CDS             5841..6035
                     /gene="ralR"
                     /locus_tag="LAF92_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HBI9793555.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            6028..6237
                     /gene="rcbA"
                     /locus_tag="LAF92_004662"
     CDS             6028..6237
                     /gene="rcbA"
                     /locus_tag="LAF92_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HBI9793556.1"
                     /translation="MYKITATIEKEGGTSTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSSRIALYSN"
     gene            6316..6531
                     /gene="xisR"
                     /locus_tag="LAF92_004663"
     CDS             6316..6531
                     /gene="xisR"
                     /locus_tag="LAF92_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HBI9793557.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            6533..>7010
                     /locus_tag="LAF92_004664"
     CDS             6533..>7010
                     /locus_tag="LAF92_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HBI9793558.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRSLIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVR"
BASE COUNT         2221 a         1511 c         1636 g         1642 t
ORIGIN      
        1 aagaaaaaac aaaaagcctc ttaaaccagc ataaatccgg tagcgtcccc ctccgggttt
       61 gtgcttcact ttatcccgtc tcattttttt atacataaaa tgtacttaaa gtactttaca
      121 atgatgaaca caaagtacat tatataccta ccaacccacc ccgccccaca gaacgccggg
      181 caatacttcg agttaccagg cagtggtcag gggttaagta gccagcccga ggcgtatgaa
      241 catgacggcg ggattcaaat tttgcagtgc agcagttagt tccgccaccc ggcgttaagg
      301 ggatagataa gatggtgcat tacgaagtag ttcagtattt gatggattgt tgcggtatca
      361 cttacaacca ggctgtgcag gctttacgca gcaacgactg ggatctctgg caggcagaag
      421 tcgctatacg tagcaacaag atgtgagatt cgcaaaatgc aaaaaatcga cctcggcaac
      481 aacgaatccc tggtgtgcgg cgtgttcccc aaccaggatg gaacgttcac tgccatgacg
      541 tataccaaaa gcaaaacatt taaaaccgaa actggtgcgc gccgatggtt ggagaagcac
      601 acagtaagct aacgattaaa acgtctactc ctgctgttcc agaataactt cataaaatgg
      661 gagtattttt cggtgacgag ataataagaa cagtttgcgc tatcactctg atgttgaatg
      721 atgcccttcc gttctaattt tttcataacc gggttacggc aaggagaagt gataataaga
      781 tttcctgttt taaggaaatc tttaaataca gcgatttctt tctcagataa acgaagcaat
      841 actcgttgct ctggtagtaa tgaataatgc ttttgaatat gcgctcgcaa tcttgagaag
      901 gaaatggcga ccacgaaaga aaaggcaaaa acgataatct gaaagagcca aggtatttca
      961 gtataagcat tgaatgcgac agtaaactct ttcggtatca gccagagagt gagaccaaaa
     1021 atgataatcg tatacataag tctttcgagt ggctcgttag caaaaaattt caacaatgga
     1081 gtaaatacat ccaacatatc aataactctc aactgtaagg gtattgaaat gttaacacaa
     1141 gctctcgctg taggagtata gccgagacca ccgaagcccg gaggtggtga aataaaaccg
     1201 ggcacaacac gaaggcgcat ttccgatatc cataaagagt cggtcttgtc tgttaaattt
     1261 aaatggtggg agtgcgcctc cggttgtaaa taacgacatt gctgtgtgta gtcctggcgg
     1321 catcagtttt tttcttgaag ttcggctgat gtccgccctt tttaaagtga attttgtgat
     1381 gcggtgaatg cggctaagcg cacgtggcac agttaaaagt catgttagtc cttattggtt
     1441 tgggtgggaa agccgactgt aattgttaac tggttgcagt cacctggagg caccaggcac
     1501 cgcatcaaca aagttcattt gtaaaaatgg agataattat gattgcacat cacttcggaa
     1561 ctgatgaaat accacgtcag tgtgtgactc ctggcgatta tgttcttcat gaaggccgga
     1621 catatattgc ctcggcaaac aatattaaaa agcgaaaact atatattcgt aacctgacca
     1681 caaaaacatg cattactgac cgcatgatta aagtcttcct cggtcgtgat ggtttacctg
     1741 taaaggcgga gtcatggtga tgactaagaa aataaaatgt gcttaccacc tttgcaaaaa
     1801 agacgttgaa gaaagcaaag ctattgaaag aatgcttcac ttcatgcacg ggattttatc
     1861 aaaagacgaa ccgagaaaat attgcagtga agcttgtgcc gaaaaagacc agatggcaca
     1921 tgaactttaa ttaattgact attcgaaact gaatttatgc cagaaatggc aggtattcgc
     1981 tcaaccttaa ttaaggagaa aaacatgatt accaattatg aagccactgt tgtaactacc
     2041 gatgacattg ttcacgaggt gaatctggaa ggaaagcgca ttggctacgt aattaaaaca
     2101 gaaaataaag aaaccccatt cactgtggtt gatatcgatg gtccatcagg caacgtaaaa
     2161 acacttgatg aaggtgtcaa aaaaatgtgc ctggtgcata tcggaaagaa tctgcccaca
     2221 gaaaaaaaag ccgaatttct ggcaactcta attgcaatga aattaaaagg tgaaatctga
     2281 aagaaatagc ctgcgtatga cgcaggctat gaacagtgtg tatccggcaa gatcattcac
     2341 tgaacaaaac gaattttaat ctgagttgag gttaaaaaac aatgagcaca aaaccactct
     2401 tcctgttacg gaaagcgaaa aaatcatccg gtgaacctga cgtcgtcctg tgggcaagca
     2461 acgattttga atcgacctgt gccactctgg actacctgat cgttaagtca ggtaaaaaac
     2521 tgagcagcta ttttaaagct gttgccacga attttcctgt cgttaatgac ctgcccgctg
     2581 aaggcgagat cgattttacc tggagtgaac gctatcaact cagcaaagac tccatgacat
     2641 gggaactaaa accgggagca gcaccagaca acgctcacta tcaaggcaat accaacgtca
     2701 acggcgaaga catgactgag attgaggaga atatgctact cccaatttct ggccaggaac
     2761 tgcccattcg ttggcttgct caacacggca gcgaaaaacc ggtaacgcac gtttcacgcg
     2821 acggactcca ggcattacac attgctcggg ctgaagaact accggctgtt actgccctgg
     2881 ctgtttccca caaaaccagc ctgctcgacc cgctggaaat tcgcgaactc cacaaactgg
     2941 ttcgtgacac tgacaaagtt ttccctaatc ctggtaattc aaacctggga ctgataactg
     3001 cttttttcga agcatacctg aacgctgact acaccgatcg aggactgctg acaaaagagt
     3061 ggatgaaggg taatcgtgtt tcacacatca ctcgcacggc ttccggtgct aatgctggcg
     3121 gcggaaacct caccgatcgc ggcgaaggtt tcgtacacga tctgacgtca ctggcgcgcg
     3181 acgtagccac tggcgtactg gcccgttcaa tggatctgga catctataac cttcatccgg
     3241 tacacgctaa acgcattgag gaaattatcg ctgaaaataa accgcccttt tctgttttcc
     3301 gcgacaaatt catcaccatg cctggcgggc tggattattc ccgcgccatc gtggttgcgt
     3361 ccgtaaaaga agcaccaatt gggatcgagg tcatccccgc gcacgtcact gaatatctga
     3421 acaaagtact gactgaaacc gatcatgcca accctgatcc ggaaatcgtg gatattgcct
     3481 gcggtcgctc ctctgccccg atgccgcagc gagtaacaga agaaggaaaa caggatgatg
     3541 aagaaaaacc gcaaccatct ggaacaacgg cagttgaaca gggagaggct gaaacaatgg
     3601 aacaggacgc aactgaacat catcaggaca cgcagccgct ggatgctcag tcacaggtaa
     3661 attctgttga tgcgaaatat caggaactgc gggcagaact ccatgaagcc cggaaaaaca
     3721 ttccatcaaa aaatcctgtc gatgccgata aattgcttgc tgcatcacgt ggtgaatttg
     3781 ttgacggaat tagcgacccg aacgatccga aatgggtaaa ggggatccag actcgcgatt
     3841 gtgtgtacca gaaccagcca gaaacggaaa aaaccagccc ggatatgaat caacctgagc
     3901 cagtagtgca acaggaaccg gaaatagcct gcaatgcctg cggccagact ggcggggata
     3961 actgccctga ctgtggtgcg gtgatgggcg acgcaacata ccaggaaaca ttcgatgaag
     4021 agagtcaggt tgaagctaag gaaaatgatc cggaggaaat ggaaggcgct gaacatccgc
     4081 acaatgagaa tgctggcagc gatccgcatc gcgattgcag tgatgaaact ggcgaagtcg
     4141 cagatcccgt aatcgtagaa gacatagagc caggtattta ttacggaatt tcgaatgaga
     4201 attaccacgc gggtcccggt atcagtaagt ctcagctcga tgacattgct gatactccgg
     4261 cactatattt gtggcgtaaa aatgcccccg tggacaccac aaagacaaaa acgctcgatt
     4321 taggaactgc tttccactgc cgggtacttg aacaggaaga attcagtaac cgctttatcg
     4381 tagcacctga atttaaccgc cgtacaaacg ccggaaaaga agaagagaaa gcgtttctga
     4441 tggaatgcgc aagcacagga aaaacggtta tcactgcgga agaaggccgg aaaattgaac
     4501 tcatgtatca aagcgttatg gctttgccgc tggggcaatg gcttgttgaa agcgccggac
     4561 acgctgaatc atcaatttac tgggaagatc ctgaaacagg aattttgtgt cggtgccgtc
     4621 cggacaaaat tatccctgaa tttcactgga tcatggacgt gaaaactacg gcggatattc
     4681 aacgattcaa aaccgcttat tacgactacc gctatcacgt tcaggatgca ttctacagtg
     4741 acggttatga agcacagttt ggagtgcagc caactttcgt ttttctggtt gccagcacaa
     4801 ctattgaatg cggacgttat ccggttgaaa ttttcatgat gggcgaagaa gcaaaactgg
     4861 caggtcaaca ggaatatcac cgcaatctgc gaaccctgtc tgactgcctg aataccgatg
     4921 aatggccagc tattaagaca ttatcactgc cccgctgggc taaggaatat gcaaatgact
     4981 aagcaaccac caatcgcaaa agccgatctg caaaaaactc agggaaaccg tgcaccagca
     5041 gcagttaaaa atagcgacgt gattagtttt attaaccagc catcaatgaa agagcaactg
     5101 gcagcagctc ttccacgcca tatgacggct gaacgtatga tccgtatcgc caccacagaa
     5161 attcgtaaag ttccggcgtt aggaaactgt gacactatga gttttgtcag tgcgatcgta
     5221 cagtgttcac agctcggact tgagccaggt agcgccctcg gtcatgcata tttactgcct
     5281 tttggtaata aaaacgaaaa gagcggtaaa aagaacgttc agctaatcat tggctatcgc
     5341 ggcatgattg atctggctcg ccgttctggt caaatcgcca gcctgtcagc ccgtgttgtc
     5401 cgtgaaggtg acgagtttag cttcgaattt ggccttgatg aaaagttaat acaccgcccg
     5461 ggagaaaacg aagatgcccc ggttacccac gtctatgctg tcgcaagact gaaagacgga
     5521 ggtactcagt ttgaagttat gacgcgcaaa cagattgagc tggtgcgcag cctgagtaaa
     5581 gctggtaata acgggccgtg ggtaactcac tgggaagaaa tggcaaagaa aacggctatt
     5641 cgtcgcctgt tcaaatatct gcccgtatca attgagatcc agcgtgcagt atcaatggat
     5701 gaaaaggaac cactgacaat cgatcctgca gattcctctg tattaaccgg ggaatacagt
     5761 gtaatcgata attcagagga ataattcagc ctggcggtgt aatgcaccgc caacttgaaa
     5821 tattttttat gagaaaaatt atgagatatg acaatgttaa accatgtcca ttttgtggtt
     5881 gtccatcagt aacggtgaaa gccatttcag gatattaccg agcgaagtgt aacggatgcg
     5941 aatcccgaac cggttatggt ggaagtgaaa aagaagcact cgaaagatgg aataaacgaa
     6001 ccactggaaa taataatgga ggtgttcatg tataaaatta ccgccactat tgaaaaggaa
     6061 ggtggcactt ctactaactg gacaagatat tcaaaatcta aactaacgaa atcagaatgc
     6121 gaaaaaatgc tctcaggtaa aaaagaagca ggcgtttcca gagagcagaa agtaaaactg
     6181 ataaatttta attgcgagaa acttcagtcc tcgagaattg cattgtattc aaattaaaac
     6241 ttcatagctg attattaata atcaacatcg ggcgtcaatt tcagtctaac attggcgcct
     6301 gccagaggtg atgcgatggc acaagtaatc tttaatgaag agtggatggt tgaatacggc
     6361 ctgatgcttc gcactggtct gggggccaga caaattgaag cataccgcca gaactgttgg
     6421 gtggagggct tccacttcaa acgagtatct cctttaggta agccagacag caaacgaggg
     6481 attatctggt acaactatcc aaagataaat cagtttatca aagactcatg atatgtctaa
     6541 attaccaaca ggtgtcgaga ttagaggtag atacattcgc atctggttca tgtttcgagg
     6601 aaaacgatgt cgggaaacat taaaaggctg ggagattaca aacagtaata ttaaaaaggc
     6661 cggaaattta agatcgctga tagttcatga aataaactcc ggtgaatttg agtatttaag
     6721 acgttttccc cagtccagca ctggggcaaa aatggtgaca acgagagtca taaaaacgtt
     6781 cggagagctt tgtgatatct ggacaaaaat taaagagaca gagttaacaa caaacacaat
     6841 gaagaaaacg aaatcacaat taaaaacact cagaataata atttgtgaaa gtaccccgat
     6901 atcacatatt cgttatagcg atatcttaaa ctaccggaat gaactgctgc atggagaaac
     6961 gctttacctg gataatccaa gatccaacaa aaaaggaaga accgtgcgca
//
LOCUS       DAEVZA010000193         6994 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEVZA010000193 DAEVZA010000000
VERSION     DAEVZA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.193"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            95..721
                     /gene="dmsB"
                     /locus_tag="LAF92_004665"
     CDS             95..721
                     /gene="dmsB"
                     /locus_tag="LAF92_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBI9793559.1"
                     /translation="MKQKQYGFYIDSSRCTGCKTCQVACKDKNQLPIGVNFRRVYEYA
                     GGSWTQAGKDIWRQDVFAYYLSVSCNHCADPACTKVCPTGAMHKRTQDGLVVVDEQIC
                     IGCRYCEMACPYGAPQYNSEKKHMTKCDGCYELIEQNKKPLCVSSCPLRALDFGPIDE
                     LREKYGDITDIAPLPQSSFTQPSITINPNPHGKPTSEYRGTIVNKSEL"
     gene            724..1551
                     /locus_tag="LAF92_004666"
     CDS             724..1551
                     /locus_tag="LAF92_004666"
                     /inference="COORDINATES: protein motif:HMM:NF016838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBI9793560.1"
                     /translation="MHELPLVFFTVLMQAVAGSFILLWLIKLIPAAQLQTSGQQFSLA
                     IFSLWPLLALAGLAALTHLGQPLRALNVLFGLKHLSPMSLEIVMVLIFSLLGLSTSFL
                     LLKKVKGAPLLLCAVLAALAAIGVLFAIANVYFLPTVSLWSTPWTVINFVFSGILSGS
                     LLSALLLYAFRNRNGSSTSFIYIMKPIWGAVLFLFILFSCGYLFALNGEVSAIPVWLK
                     AIQVIRILLLASAFLCCLLMNLSTQITGKLAAVTTAVIIAELLGRVFFYELILLQQL"
     gene            1590..4013
                     /locus_tag="LAF92_004667"
     CDS             1590..4013
                     /locus_tag="LAF92_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBI9793561.1"
                     /translation="MSTQQNITQAVDHSDLSRRRFLKLSSFLGMGAYLSTHSMNAIAA
                     AVIDTHVGEVAKWHNCWAHCHSSCLLKIVTENGEIKRIETDDQGDDTFGRHQVRACLR
                     GRSLHKRTFAPDRLKYPMKRIGKRGEGKFKRISWEEALTEVHDKLSHIIREYGNQAIF
                     SRIGYGKPDGSYHYVPRFLNMIGGYLSPEGNYSSHQIDTASQYTYGDKSYTFGSAPTE
                     MARSDLIVYFGNNPANTRMSGGSDTYHYQWYKKQNSARTIVIDPQYTDTACGREDLWL
                     AIRPGSDAALVEAIAWVLITEGFTDDDFLKTHCYGYDEEPGDSEKNIPPLDKRLGYKA
                     YILGEGIDGIAKTPKYASAICGIPEEQIYQLARELGHAKAPYIAQGWGPQRQAAGEQT
                     ARAIFMLPILLGKIGLPGTNNGASDFLFHTTDVMTMPTGKNPVSAKVPCFKWLEAVER
                     GHEMTALTDGIKGAPKLDTDVKCVFMYQGNWLLNQHSDCNATAKILADESKLEFIFVM
                     DNHMTASAKFADILLPDITWLENSRIVAFSTHLTMTEHVIDPMYECRHPYDVFADLAD
                     KFGLREKFTEGRSWDDWQRYLYNQSRLKHTNFPPIEILEREKTVRLPFDPLAPRYTLK
                     AFRENPALNPLKTESGKIQIYSHKLAKLAETWTLPAGDTITPLPQYTPTWESYADEAL
                     REQYPLQLIGFKSKGRPHSTFHNVPWLREVIDDVLMINPIDAKARHLAQGQMVHVYNA
                     RGTIQIPIRITPRIMPGVVAMGEGAWYQPDEKGIDIGRCINTLTRLKPTALAKANPQG
                     TNLVEVKPI"
     gene            4096..4743
                     /locus_tag="LAF92_004668"
     CDS             4096..4743
                     /locus_tag="LAF92_004668"
                     /inference="COORDINATES: protein motif:HMM:NF014651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HBI9793562.1"
                     /translation="MSHRIETEAITERELSLVSLSLLYHFFCFEPQKALINNMLNSDI
                     LNLWHSKWDMDIHETNTFLQNQDALERLQADHLALFVGIGMPKAVPWGSVYLTEENLL
                     LQRSTHALTAFLKSHRIILCTDEQQISDHIGLCLSVLALLLKQWGDYPEETTERTIRE
                     FLRDHLLPWSGRFCELLTAHAETPLYALLGKVTKRILKDQQHQWQITPRPRTLYY"
     gene            4936..6603
                     /gene="ettA"
                     /locus_tag="LAF92_004669"
     CDS             4936..6603
                     /gene="ettA"
                     /locus_tag="LAF92_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBI9793563.1"
                     /translation="MAQYVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDTDIEGEARPQPGIKIGYLPQEPKLNPEQTVREAVEEAVSEVKNALT
                     RLDEIYAAYAEPDADFDKLAKQQGEMEAIIQSHDGHNLDNQLERAADALRLPPWDAKI
                     EHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDKRLEQEASSEAARRKSIEKELEWV
                     RQNPKGRQAKSKARLARFDELNSVEYQKRNETSELFIPPGPRLGDKVLEVQNLKKSYG
                     DRLLIEDLSFAIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGDTVKIASVD
                     QFRDSMDNSKTVWEEVSGGLDIMRIGNFELPSRAYVGRFNFKGTDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYGDEGKVEFFEGNFTEYEDWKKRTLGAEALEPHRIKYKKIAK"
     gene            6719..>6994
                     /locus_tag="LAF92_004670"
     CDS             6719..>6994
                     /locus_tag="LAF92_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005175332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793564.1"
                     /translation="MPANVLLLGATGLIGSHLLAMLQSDPRIERIYAPTRHPLSNAGN
                     KVINPTGSDVQILLDTLTEPVDAAFCCLGTTQKTAGSRAAFRFADITL"
BASE COUNT         1940 a         1624 c         1573 g         1857 t
ORIGIN      
        1 tttacatgtg gaaatcccgt atagaaacaa tccacagcat caaccactat gcctccaaac
       61 tgtaataacc attcactcta atttggtggc agctatgaaa caaaaacaat atggcttcta
      121 tatcgacagc agtagatgta caggatgtaa aacctgtcag gttgcctgta aagataaaaa
      181 tcaactgcct attggtgtca attttcgtcg ggtatatgaa tacgccggag gtagttggac
      241 tcaagccgga aaagatatct ggcgacaaga cgttttcgct tattacctct ccgtcagctg
      301 taatcactgc gccgatccgg cctgtaccaa agtttgccct accggcgcta tgcataagcg
      361 tacacaggat ggtttagttg ttgttgatga gcaaatttgt atcggttgtc gatactgcga
      421 aatggcctgt ccgtatggag cgccgcagta taacagcgag aagaaacaca tgacaaaatg
      481 tgatggctgt tatgaattaa tcgaacaaaa taaaaaaccg ctgtgtgttt catcgtgtcc
      541 gttacgggcg ctcgattttg ggccaatcga cgaactaaga gaaaaatatg gtgatattac
      601 tgatatcgcc ccacttcccc aatcgtcgtt tacccaaccg tctatcacga tcaatcccaa
      661 tcctcatggg aagccaacgt cagagtatcg tggaacgatt gttaataaat cggagctata
      721 actatgcatg aattaccttt ggtcttcttt accgtactta tgcaagccgt tgccggtagc
      781 tttattctgc tttggttaat taagctgatc cctgccgctc agctacaaac ctcaggccaa
      841 caattttcac tggcaatatt ctctctttgg ccgctgttag cactggcggg gctcgccgcg
      901 ttaacacatt taggccaacc attaagagcc ctaaacgtac tctttggcct aaaacatctt
      961 tctcccatga gcctcgagat cgtcatggtg ctcattttta gtttgcttgg cctaagcacc
     1021 agtttcttac tattgaaaaa agtaaaagga gcgccactgc tcctgtgcgc tgttctggcg
     1081 gcactggcag ccatcggtgt tctatttgcc atcgcaaacg tttatttttt accaacagtt
     1141 tctctttggt ctacgccgtg gaccgttatt aactttgtat ttagcggtat tttgtccggc
     1201 tcattattaa gcgcccttct gttgtacgca tttcgtaaca gaaacgggag ttcaacatcg
     1261 ttcatataca tcatgaaacc catttggggc gcggtactct ttctatttat cctgttttcc
     1321 tgtggctatt tgtttgctct aaacggagaa gttagcgcaa ttcctgtatg gcttaaagct
     1381 attcaagtta ttcgaatcct gttattggca tccgcatttc tctgctgcct tttaatgaat
     1441 ttatccacgc aaataacggg caagttagcc gctgtaacaa cggctgtcat tattgccgag
     1501 ctcttaggaa gagtcttttt ctatgaactt attttactgc aacagcttta aggcaatcgg
     1561 acttaacgcc gcgatataag gattttatta tgtctacaca acaaaatata acgcaagcag
     1621 tcgaccattc tgatttatcc cgcaggcgct ttttaaaact gagctctttt ctcggtatgg
     1681 gcgcctattt atcgactcac tctatgaatg ctatcgctgc ggctgttatt gatactcacg
     1741 taggtgaagt tgccaaatgg cacaattgct gggcacattg tcattccagt tgcttattaa
     1801 aaatcgtgac tgaaaacggt gaaataaaac gtattgaaac ggacgaccaa ggcgatgata
     1861 catttggtcg ccatcaagtc agagcttgtt tacgcggccg atctttgcat aaacgaacct
     1921 tcgctcccga cagattaaaa tacccaatga aacgcatcgg caaacgtggc gaaggtaaat
     1981 ttaagcgcat cagttgggaa gaagcactaa cggaagtgca cgataagcta agtcatatca
     2041 tccgtgaata tggtaatcag gcaatcttca gccgaatcgg ttacggaaaa ccggacggtt
     2101 cttatcacta tgttcccaga tttctgaata tgatcggcgg ctacctatcg ccggaaggta
     2161 attacagtag tcaccagatt gatacggctt ctcaatatac ttacggcgat aaatcttata
     2221 cgttcggcag cgcaccaacg gaaatggcgc gttcagatct gattgtttat tttggaaata
     2281 atccggccaa tacgcgaatg agcggtggca gcgatactta ccattatcaa tggtacaaaa
     2341 agcaaaactc cgccagaaca atcgttattg atcctcaata cacagatacc gcctgtggtc
     2401 gggaagatct ttggctggct attcgcccgg gaagtgatgc agcattagtt gaagcaattg
     2461 cgtgggtact gataacggaa ggttttaccg atgacgactt tctgaaaaca cattgttacg
     2521 gttatgacga agaaccgggt gatagcgaaa aaaatattcc tccgttagat aagcgtttgg
     2581 gatacaaagc ttatattttg ggtgaaggca tcgacggtat cgcaaaaacg ccaaaatacg
     2641 ccagcgcaat ctgcggcatt ccggaagaac aaatttatca gttagcacgt gaacttggcc
     2701 acgcgaaagc gccctatatt gctcaaggtt ggggccccca gcgtcaggct gccggagaac
     2761 aaacggccag agcaatattt atgctgccaa ttttgctggg taaaatcggc ttgcccggta
     2821 cgaataacgg cgcatcagac tttttgttcc acacaacaga tgtcatgaca atgccaaccg
     2881 gaaaaaaccc ggtttcggca aaagttccct gctttaaatg gctggaagcc gtagaacgtg
     2941 gccatgaaat gacggcatta accgatggga tcaaaggcgc acctaaatta gataccgacg
     3001 taaaatgcgt ctttatgtat caaggcaact ggctgttaaa tcagcactct gactgcaatg
     3061 caacggccaa aatattggcc gatgaaagca agttagagtt tattttcgtg atggataatc
     3121 acatgacagc cagcgctaaa tttgcggata ttttactacc ggatattact tggctggaaa
     3181 actctcgtat tgtggccttc tcaacgcatt tgactatgac tgaacatgtc attgatccta
     3241 tgtacgagtg ccgccatcct tacgatgtct ttgccgatct tgccgacaaa tttggtctcc
     3301 gcgaaaaatt tactgaaggg cgcagttggg atgactggca acgctatctg tataaccaaa
     3361 gccgtttaaa acacacgaat tttccgccta tcgagatttt agaacgagaa aaaaccgttc
     3421 gtcttccgtt tgatcctctc gcaccgcgat atacgttaaa agcgtttcgg gaaaatccgg
     3481 ccctcaatcc actgaaaaca gaatcaggta aaattcagat ttacagccat aaactagcca
     3541 aacttgcgga aacatggacg ctgccggcgg gagatacgat tacgccattg ccgcaatata
     3601 cgccgacgtg ggagagctat gccgatgagg cattaagaga gcaatatcct ttacaactca
     3661 ttggatttaa atcaaaagga cgcccacact caacgtttca taacgttccg tggctgcggg
     3721 aagtcattga cgatgtttta atgattaatc ctattgatgc aaaggctcgt catcttgctc
     3781 aagggcagat ggtgcatgtt tataacgcac gcggaacgat tcaaattcca atacgcatta
     3841 cgccaagaat aatgcccggc gttgtagcga tgggcgaagg tgcatggtat cagccggatg
     3901 agaaaggcat tgatatcggt cgatgcatta ataccctcac tcgtttaaag ccgacggcct
     3961 tagcgaaagc caaccctcaa ggaaccaatc tggtcgaagt caaaccgatt taaattcaac
     4021 gttagcctct caatggaggc taactcattg atttcgtttt agccggaaat ctatctcttt
     4081 ttataggtga ataaaatgag ccatcgtata gaaactgaag ctattactga gcgtgaattg
     4141 tcactggtca gcttatccct tttgtatcat ttcttttgct ttgaaccaca aaaggcacta
     4201 atcaacaata tgttaaacag cgatattctt aatctttggc acagtaaatg ggacatggat
     4261 attcacgaga caaacacctt tttacaaaat caagatgctc tcgagcgttt gcaggctgac
     4321 catttagcct tattcgttgg cattgggatg ccaaaagccg ttccatgggg atcggtttat
     4381 ctgacggaag agaacctgct attacagcgc tcaactcatg cattaaccgc ttttttgaaa
     4441 tcccaccgca ttatactttg taccgatgaa cagcaaattt ctgaccacat tggattatgt
     4501 ttatcagtac tcgccttgct attaaaacaa tggggagatt atccggaaga aaccaccgag
     4561 cgcacaatac gcgaattttt acgcgatcat ttattgccat ggagtggccg tttttgcgaa
     4621 ttgctcaccg cccatgctga aacgcctcta tatgcattat taggaaaagt cactaaacgg
     4681 atattaaaag atcagcaaca tcaatggcag attaccccgc ggccccgcac gctatattat
     4741 tgatcttaaa cttttacaaa cggattttat ctaatggggg ccatgaggtt gccattcgtg
     4801 tgccggcgtt caaaagtctc aactcacggt tttatcaggt tgaggaaaat atgccgatca
     4861 ccatggcaaa gtcttcccct ccatgctcta atcggcgcca ttaagaaacc ccgtaattaa
     4921 gaggctccta atactgtggc acaatacgtc tatactatgc atcgtgtcgg aaaagttgtt
     4981 cctccgaagc gacatattct aaaaaatatc tctctcagct tcttccccgg cgccaaaatt
     5041 ggtgtgcttg gtttaaacgg cgcaggtaaa tccacgctat tacgcattat ggccggtatc
     5101 gacaccgata tcgaaggtga agcgcgtccg cagccgggca ttaaaattgg ttatttgccg
     5161 caagaaccta agctgaatcc tgagcaaacc gtaagagaag cagttgaaga agccgtcagt
     5221 gaagtcaaaa atgccttaac gcgtttagac gaaatttatg cggcatacgc agaaccggat
     5281 gcagacttcg ataagctggc taaacagcaa ggcgaaatgg aagcgatcat tcaatctcac
     5341 gatggacata atctggataa ccagttggag cgtgctgctg atgcattgcg ccttccgccg
     5401 tgggacgcca aaattgagca tttatccggt ggtgaacgcc gtcgcgttgc actttgtcgc
     5461 ctactgctgg aaaaaccgga catgttactg ctcgacgaac caaccaacca cttggacgca
     5521 gagtccgttg cctggctgga acgcttcctg catgactatg aaggcaccgt tgtagccatt
     5581 acccacgacc gttacttcct tgataacgtc gcaggctgga ttctggaact tgaccgcggt
     5641 gaaggtattc catgggaagg taactattct tcatggctgg agcaaaaaga taagcgtctg
     5701 gagcaagaag cctcttccga agccgctcgc cgcaaatcta ttgagaaaga acttgagtgg
     5761 gttcgccaga atccaaaagg ccgtcaagct aagagtaaag cgcgtcttgc ccgctttgat
     5821 gagctcaata gcgttgaata tcaaaaacgc aatgaaacca gcgagctgtt cattccgccc
     5881 ggccctcgtt taggcgataa agtgctggaa gttcagaatc tgaaaaaatc ttatggcgat
     5941 cgtctgctaa tagaagattt atcattcgcg attccaaaag gtgccattgt cggtattatc
     6001 ggcccgaacg gcgctggtaa atcaacgctg tttcgcatga tttccggtca ggagcagcct
     6061 gacagcggca caatcacttt aggtgatacc gtaaaaatcg cctccgttga tcagttccgt
     6121 gacagtatgg acaatagcaa aacggtttgg gaagaagttt ccggcggtct ggatattatg
     6181 cgcattggta actttgaact gccaagccgc gcttatgtcg gtcgcttcaa cttcaaaggc
     6241 accgatcagg gcaaacgcgt tggcgagctt tccggcggcg aacgcggtcg cttacattta
     6301 gctaaactgc tacaagttgg cggaaatatg ctgcttctcg acgaaccgac taacgatctg
     6361 gatatcgaaa cgctgcgtgc gctcgaaaac gccctgttag agttcccggg ctgcgccatg
     6421 gtcatttccc atgaccgctg gttccttgat cgtatcgcta cgcacatcct tgattacggc
     6481 gatgaaggta aagtggaatt ctttgaaggt aactttactg aatacgaaga ctggaaaaag
     6541 cgtacgctgg gtgcagaagc gcttgaacct catcgtatca aatataagaa gatcgccaaa
     6601 taattctgat ctattcttat taacggatgt tttatcaata cacacattaa cgccccttaa
     6661 tttaggggcg ttttttcagg ttgagcgtaa cgggcttaat cacttgagga gagcagagat
     6721 gcctgcaaac gttttattat taggcgcaac cggattaatc ggcagtcatt tactcgctat
     6781 gctgcaatcc gatccgcgca ttgaacgcat ttacgctccg acgcgccacc cgctgtcaaa
     6841 tgcgggtaac aaagtgatta acccaacggg cagtgatgtt caaattctgc ttgatacgct
     6901 gactgaaccc gttgacgccg ctttttgctg tttgggaacc acacaaaaaa ccgccggtag
     6961 ccgcgcggct ttccgctttg ctgatattac actt
//
LOCUS       DAEVZA010000194         6823 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEVZA010000194 DAEVZA010000000
VERSION     DAEVZA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.194"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            286..330
                     /locus_tag="LAF92_004671"
     CDS             286..330
                     /locus_tag="LAF92_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793565.1"
                     /translation="MFSKLAQSSIKAMF"
     gene            583..906
                     /gene="iraM"
                     /locus_tag="LAF92_004672"
     CDS             583..906
                     /gene="iraM"
                     /locus_tag="LAF92_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBI9793566.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGERKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(1009..1161)
                     /locus_tag="LAF92_004673"
     CDS             complement(1009..1161)
                     /locus_tag="LAF92_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003840851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HBI9793567.1"
                     /translation="MGWTEAADLIVKGMEGAINAKTVTYDFERLMEGAKLLKCSEFGD
                     AIIKNM"
     gene            complement(1642..2376)
                     /locus_tag="LAF92_004674"
     CDS             complement(1642..2376)
                     /locus_tag="LAF92_004674"
                     /inference="COORDINATES: protein motif:HMM:NF024098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBI9793568.1"
                     /translation="MNIYIDESIHEEFGFMILAYVVCNQDPQSDIYKILSEHSVDEHH
                     SCARMDKSESLRNLRSNLISYLNANCRWGAFIMPSEYRHRSYEDVSFLLSLLIKNFPD
                     RKGNIFFDEGIINPTEALSLCSDEVKVITCSSHEVCGIQLADLVASFSGVRLRETITG
                     KAKILTYGYDFGYEPPIESPLGFELFATLRQSMLRGNEPLGNEMPEFATFKTINKGLV
                     LSKNLTTEFSQLALKTFGEVYLGCIH"
     gene            complement(2870..3100)
                     /locus_tag="LAF92_004675"
     CDS             complement(2870..3100)
                     /locus_tag="LAF92_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793569.1"
                     /translation="MCCVVFLKNSQTIPIEWIKPFDFAEKLLSEFEANLIYWNKPELN
                     TQHMKKEPTFEYGQVHAQNVTGATYFWHDKFI"
     gene            3945..5147
                     /locus_tag="LAF92_004676"
     CDS             3945..5147
                     /locus_tag="LAF92_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBI9793570.1"
                     /translation="MSLRFRQTFTLFPGVRLNIGKRGISASIGVPGATVNVGKKGVRA
                     TVGLPGTGLSYTTPTLPYDDGYSVTNPLNPAPTEPHLESGSSPENTPSNAKIYMPVAG
                     MNEISSASVEVLTSTSLLPLRDLIAKAREQRAEIKADLQEALAEESKQKSELARRKSS
                     LFRWFYKRRIAELETLLPLTQAEISRLVSWEDSTKIAITFESSDASQRAYAAMVHAFD
                     MLKSSVKKWDITADKATDQFAERTLATRSVNRHPVTFDFSSTDLIQFTGRAMRFENVN
                     GDDILLYPGVAVIPRADGAFALIDLRELQISSEYLRFHEEEGVPSDSRIDGYTWAKTN
                     KNGSPDRRFKDNYQIPICIYGNITFHSQTGVTEEYMVSNADAAHAFAEAVKRYQISLT
                     ETEALVQA"
     gene            5160..6144
                     /locus_tag="LAF92_004677"
                     /pseudo
     CDS             5160..6144
                     /locus_tag="LAF92_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700311.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
     gene            complement(6248..>6823)
                     /locus_tag="LAF92_004678"
     CDS             complement(6248..>6823)
                     /locus_tag="LAF92_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI9793571.1"
                     /translation="RMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTYIAP
                     PDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSPEGE
                     FQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQAANEHIAPLQDAVDLEIATEEEASL
                     LAAWKKYRVLLNRVDTSVAPDIEWPAVPVME"
BASE COUNT         2186 a         1374 c         1223 g         2040 t
ORIGIN      
        1 ccataaataa tcaccatgcc aatactatca aattgatatt tgaaatgaga tcacttgact
       61 ttctatacgt tattttataa cggttaatat atttataaaa acaacgggtg tgccatacgc
      121 ccgtttcaat acttaacgca catgtgtttt ggtttagtca tcatccagtt atatgtattt
      181 tagccaggaa caggttaaac ctttcctata taactcaaaa attgaaacct tattctcatg
      241 tcatgcttat attcattatt atcgttatat aaaaaggcaa ccataatgtt tagcaaattg
      301 gcacaaagta gcataaaggc tatgttttaa ttacaggatg ttcagtcatt tgaatgtata
      361 acattatagc taaacaaatc taaaacgaag tgaataattt attgttttca ctaaatctca
      421 ttttgtttaa catccattga gattccttgc tttaaatttt gttttatata agcaatcgtt
      481 ttaattaatt tattttttga aggggtaata tactcatatg caaaataaag aaatgaacat
      541 ccaaatgaac tatattaaat accgtgggat aagacataac aaatgaagtg gatagtaatt
      601 gacacgataa ttcaaccttc atgcggcata tctttttcag ccatatgggg taatatgaaa
      661 atgatcatct ggtatcaatc tactatattt ctccctcctg gcagtatatt tacaccggtt
      721 aaatctggaa ttatccttaa agataaagaa tatcctatta ctatttataa catcgcacca
      781 ttcaacaagg atttatggag tctactcaaa agcagtcaag agtgtcctcc aggagaaaga
      841 aaaataacaa ataaatgttt gcataatagt tgcattataa aaatatgccc atatgggctc
      901 aagtaaaggg ttttatataa atatgctcga tatgctatta ataataccta cataaataaa
      961 acaacgggcg tgttatacgc ccgttgcaat attaaacaca tgtagtgatt acatgttctt
     1021 gatgatcgca tcaccaaatt ctgaacattt cagcagttta gcgccttcca tcagacgctc
     1081 gaagtcatag gttacggtct tcgcattgat tgcgccttcc atacctttaa caatcaggtc
     1141 tgcggcttca gtccagccca tatggcgtaa cagtatataa aaacattaag ttcaattcat
     1201 tgatatttaa tgataaaaat tattttattg tctttttatt agccaaaatc aactttattc
     1261 aaccgtttga tttcattgac gttcgttttt attttgggga aaggttttct ctaccattta
     1321 tatccaaatt acaagtctac attttattaa catctcaata catgtgaggc cttgcagacc
     1381 tttgtgagac cttatgtgtc tctgttttgt cccaccttgt attacgactt gcatagccaa
     1441 tgaagataaa tgtgacgaca aacggcgcgg cagtcttctt ttccttcata ctttccccac
     1501 ccagcatgca taccttctac cataactgta gtgaatgtct gttatgagcg aaaagcggaa
     1561 gtcagttgta tcatcgtcac ctatattctt aggaacacct agcttaattt tgctagtaat
     1621 tgataaatag taatattaca tttaatggat gcaacctaag tacacttccc caaaggtttt
     1681 taatgccaac tgactaaatt cagttgttaa gtttttcgac aaaaccaatc ctttattaat
     1741 agttttaaat gtggcaaatt caggcatctc attcccaaga ggttcatttc ccctgagcat
     1801 agattgtcga agtgtagcaa aaagctcaaa tccaagcggg gattctattg ggggctcata
     1861 gccgaagtcg taaccgtaag ttaaaatttt tgcttttcct gttattgttt ctctcaacct
     1921 aacaccacta aaagatgcta ctagatcagc aagttgtatc ccacaaactt catgggatga
     1981 gcatgttata actttgactt catcagagca tagactgaga gcttctgtag ggtttatgat
     2041 accttcatcg aaaaaaatat tccctttcct gtctggaaaa ttcttaatca ataagcttaa
     2101 taaaaaagac acatcctcat aagaacgatg gcgatactca cttggcataa taaatgcccc
     2161 ccatcgacaa tttgcgttta agtaggaaat aagattactc ctcaaatttc ttaaagattc
     2221 agatttatcc attcttgcgc atgagtgatg ttcatcaact gaatgttctg aaagaatctt
     2281 gtatatatca gactgaggat cctgattaca aactacataa gccaagatca taaacccaaa
     2341 ctcctcgtgt atcgactcat caatgtaaat attcaaagcc ttatcctttc aatgatatat
     2401 tatttttatg ttagattata ggcatggttc attgaattac aacgccattt ttgagttaaa
     2461 aaatcctctg aatatattaa tttatcattt tgcgtgtgac ataatgataa acatgctgaa
     2521 tattagttaa aatgtccgct tctggcactc aactgccata ggcgggccca agggcagctt
     2581 cgaacgcagg ctgtatgaaa actctgattg ctgacagaag gtcacgcgtt tagatgactt
     2641 cttagaaaat ttacatagaa agatgatgca gattttactt aaaagcgcag gcgtcagcct
     2701 cttttgcatt cacctctgaa gcttaaaccg ttttcacaca gtctgagcga attacagacg
     2761 caatactaag tttattgcgc ccttgagcac aatcttgacc aatatgtaga actcattgga
     2821 aaaaaagtgc ggaaaataga gaattaactc aagttttgtt tatatagttc taaatgaatt
     2881 tatcatgcca aaaatacgtt gcgccagtta cattttgtgc atgaacttgc ccatattcaa
     2941 atgtaggttc tttcttcata tgttgcgtat tcaactctgg tttattccaa taaatcaagt
     3001 tcgcttcaaa ttcactcaat agtttctcag cgaaatcaaa aggcttaatc cattctattg
     3061 ggatggtttg gctgtttttt aaaaaaacaa cgcagcacaa atatatacgc tttgacaaat
     3121 tgtcatattt aggatggtgt aacttagtaa ttgcgctatg gcctgaagaa aatcgtgagc
     3181 tcttcgatga tgtcttgccg atgtaaacaa gcctttcatt ttcatcatct ccaactgtaa
     3241 tcaaataaat aggataacaa gctagagggg cctcaccatc acaatttgac gataagtaat
     3301 tttggttaga ctgccatgaa atatctaggt tttgggctga aagtccaata gtttcatgta
     3361 gtgcatatga ggattggaga actagaaaac atcctatctc tttttcagga attagtaatg
     3421 aagggtcttt caaacttctg cgaagataat tttcattcga tccgtattcg aggggtctgt
     3481 aagttaattc ataagccata acatcgatcc tttaaatttc ataacgatat gttaagggat
     3541 ggatgacctg ctggtcaatg tgcctaggga ctagccatgt cagcttctgg cacatagcag
     3601 ccctagagac agtggcgtaa agtcatggag gaccgatggg aggaggcaca aatcctccca
     3661 tacaaaaaat acgcaaaatc gataacagct tgaaatcatt caatattctc actatcagaa
     3721 gctgaccagc cagccgcagc actttcttgc agacgacgag cctgcggttt caactccacc
     3781 agcccaccaa tcattattgg gcttttacgt ttttcttcac ctgcaagcaa accgttgtat
     3841 catggcggaa tgaagatgag ctaggaataa tcttatgaat cagctcaata ttcatataaa
     3901 aacatttcga actttggtgt acaaaactac ataggtataa ctttatgtct cttcgtttca
     3961 gacaaacctt tactctattt cctggcgttc gactcaacat tggcaagcgt ggaataagtg
     4021 caagcattgg cgtgcctggc gcaactgtca atgttggtaa aaaaggggtt agagcaactg
     4081 tcggattacc gggcacaggc ttatcttata ctacacctac cttgccctat gatgatggat
     4141 actcagttac caatccatta aatccagccc ctacagaacc tcatttggag tctgggtcat
     4201 ctccagaaaa cacaccatcg aacgctaaaa tatatatgcc tgtagctggc atgaatgaaa
     4261 tatccagcgc ttcggtagaa gtccttacaa gtacctccct tttaccttta cgagatttga
     4321 ttgctaaagc acgagaacaa agagcagaga taaaagcaga tcttcaagag gctcttgccg
     4381 aagaatcaaa acaaaaaagc gagctggctc gacgcaaatc aagtctattc cgttggtttt
     4441 acaaacgacg catcgcagaa cttgagacac tactccccct aacccaagct gagatatctc
     4501 gcctagtttc atgggaagac agcacaaaaa tagccataac attcgagagc agtgatgctt
     4561 cacagcgcgc atatgcagcg atggtccatg catttgatat gttaaaatcg agtgtcaaaa
     4621 aatgggatat tactgcagat aaagctacag accagtttgc tgaaagaaca ttagcaaccc
     4681 ggtctgttaa tcgtcaccca gttacctttg attttagttc aacggatctc attcaattta
     4741 cagggcgtgc gatgaggttt gaaaatgtaa atggagacga tattttgctt tatcctggag
     4801 ttgcagtcat accacgagct gatggagcat tcgctctgat tgatttacgc gaattacaaa
     4861 ttagttcaga atatctaaga ttccatgagg aagaaggtgt tcccagtgac tcacgcatag
     4921 atggttatac atgggcgaaa acgaataaga atggctcacc agaccgtcgt tttaaagaca
     4981 actaccaaat ccctatttgc atttatggaa atattacttt ccattctcaa acaggggtaa
     5041 ctgaagaata tatggtatca aatgcagatg ccgcgcacgc ctttgctgag gcagtaaaac
     5101 gctatcagat ttcactcaca gaaacagaag cgttggtaca agcctaattt atcgccgata
     5161 tggggacgaa tcgtagcccc ccctcgaaca aaaaaaacct taagaaccag actattatgg
     5221 ctggttctta tacggaggat ttgttattgt acaactcttc tatctggtat atttacggat
     5281 agaactgttc aggctactca atccttttta agtacaaatg aacagataga aatagttata
     5341 ttgcaaggag ctatcatgca cactgagtat atatggcgat accttgacat agagaaattc
     5401 tctatgttac tcgaacaaaa tgctttattt ttttgctcag caaaaaattt tgaagatcca
     5461 ttcgaaggtg aatttgcttg gggacatact ggctataaaa aattcattga gacacaggaa
     5521 aaactatgcg ctactcatgg tgccggcatg gacttagatc ctttcatggc attcaattta
     5581 aagacactta aggaaataag cgagaggaca tatataagtt gctggcattg taacgaacat
     5641 gaatctgaag ctatgtggaa gctgtattgt aaaaaccccg ctaaaggcgt tgtcataaaa
     5701 tcaaagaaaa aaaacctcca aagccaactt gaaaataata atttaaacaa actacaatta
     5761 aaacacgtaa aatacgtcaa aaatttctgg ataaaacaat acaatccaga atcagatgta
     5821 ttctttaaca agagaccatc tttcgaatac gaaaaagagt ttagagctat ttttcaagag
     5881 aataccttta acactccacc tgtaaaggga aaactagtcc ctgtatctct taacaatctg
     5941 ttggaagaaa taaggatttc acctttcgca gataaggatt tcaaaaatga agttttacaa
     6001 atttcaaaaa aatatggact agatctaaaa gtcaaaaact ctgaaattga aatgcatccc
     6061 atcatgcatg ttgaggaaga aataattgca gaaggcccca attggtatca gtcaaaaatc
     6121 agattggcaa tacgtgatgc ttaatcttat attgaagttt tttatgtctc ctgctgaaac
     6181 tagctaaaat ttaccaagcc gcagcacgtt attgcataca acgtgtatgc ggcgtatctc
     6241 aaaacgatta ctccataaca gggacagcag gccactcaat atccggtgct actgatgtat
     6301 caacacggtt cagcaacacc cgatacttct tccatgctgc cagcaacgac gcctcttctt
     6361 ccgttgcgat ctccagatcc acagcatcct gaagtggcgc aatatgctca ttcgctgcct
     6421 gcatcaggct gttttttgtt tcttccgcct cccggatccg gaatagtttt tctgcttctg
     6481 catctttcac ccaggctgtg ccgttccact tctgaaactc cccttccggg gataaccagg
     6541 tgacattttc cggtaatgag ccgagttcag aaataaataa cgcgtcgccg gaagccacgt
     6601 cataaaccgt tttaccccga tgatcttcaa cgagatgcca cgatgcctca tcactgttga
     6661 aaacagccac gaagccagcc ggaatatctg gcggtgcaat ataggtactg tttgctggca
     6721 gacctgtatg aggcggaata tatgcgtcac cttcaccaat aaattcatta gttccggcca
     6781 gcagattata aatttttatg gtccgtggtt gttcactcat tct
//
LOCUS       DAEVZA010000195         6799 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEVZA010000195 DAEVZA010000000
VERSION     DAEVZA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.195"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(202..2775)
                     /gene="clpB"
                     /locus_tag="LAF92_004679"
     CDS             complement(202..2775)
                     /gene="clpB"
                     /locus_tag="LAF92_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBI9793572.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2905..3636)
                     /gene="pgeF"
                     /locus_tag="LAF92_004680"
     CDS             complement(2905..3636)
                     /gene="pgeF"
                     /locus_tag="LAF92_004680"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBI9793573.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRVGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3633..4613)
                     /gene="rluD"
                     /locus_tag="LAF92_004681"
     CDS             complement(3633..4613)
                     /gene="rluD"
                     /locus_tag="LAF92_004681"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBI9793574.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4748..5485
                     /gene="bamD"
                     /locus_tag="LAF92_004682"
     CDS             4748..5485
                     /gene="bamD"
                     /locus_tag="LAF92_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBI9793575.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5756..6097
                     /gene="raiA"
                     /locus_tag="LAF92_004683"
     CDS             5756..6097
                     /gene="raiA"
                     /locus_tag="LAF92_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBI9793576.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6200..6325
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6201..6248
                     /gene="pheL"
                     /locus_tag="LAF92_004684"
     CDS             6201..6248
                     /gene="pheL"
                     /locus_tag="LAF92_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HBI9793577.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6347..>6799
                     /locus_tag="LAF92_004685"
     CDS             6347..>6799
                     /locus_tag="LAF92_004685"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBI9793578.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPY
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQA"
BASE COUNT         1721 a         1804 c         1601 g         1673 t
ORIGIN      
        1 aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt ggtggcgcat
       61 tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg ttcgctcatt
      121 tttcaggcaa aacatcttaa atatagtctt ttccgtctaa cttatagaca aaaacgagcc
      181 ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat taacttccag
      241 gcgaatcact ttacctggaa ccaattcacc agacagtatt tgctgtgcca gcgggttttc
      301 gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat cgtaaccgtt
      361 ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac gttcttccag
      421 acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct gttcacccag
      481 cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga agttatggct
      541 taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga agcgttcctg
      601 aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga agtcgaccgt
      661 tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa tgttgaagac
      721 atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac gacgacgcac
      781 agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg caccaaccag
      841 acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca tcgcctcgtc
      901 gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca cacccgttgg
      961 gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg cacggctacg
     1021 gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc gatggtgcag
     1081 ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag aaaccggaat
     1141 ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat tacgcaacag
     1201 acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca gttccgggat
     1261 tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag cctgttcaat
     1321 agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa gcgatgcctt
     1381 ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc tcagttcttc
     1441 gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta acgcctgttg
     1501 ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg gttttgagtc
     1561 aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt tatccggcag
     1621 ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg ccgggtcagt
     1681 aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa tcgcaatggt
     1741 atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca gcgcagcatc
     1801 tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt gcaattcacc
     1861 acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg ctttacccgc
     1921 gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct gtttggcaag
     1981 atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg ccccagccac
     2041 cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg gcacttcgcc
     2101 gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc cgggttcacc
     2161 aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac ggcgaatttc
     2221 ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg taaggtcgat
     2281 ggtatatttt ttcaaagcct ggcgttggtc ttcagcacct tgatcgttca cgctttcacc
     2341 tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg ctgctttcag
     2401 gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt ctgacgagat
     2461 aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc gcaccagatc
     2521 ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat ttaatgcctg
     2581 attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata aaggactaac
     2641 cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga taaattggtt
     2701 gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt tattagtaag
     2761 acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga gattaaatga
     2821 ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa aatcacgcgt
     2881 accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca tacgaccggt
     2941 ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac aacggtcgcc
     3001 gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct gataaatatc
     3061 cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt ctactgccat
     3121 aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg ccggccctaa
     3181 ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt ccagcacgcc
     3241 tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttccca ctcgattgca
     3301 aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccag gcgtattgct
     3361 ataagaggca tcagctcgtt ttgaggcata aggttcgcca gtaagcttaa gcacatcttt
     3421 gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag caaaaagtcg
     3481 cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga ggttgagtga
     3541 gtcatacggg ggcaagctca cgccgccgat acgagtagag ctacaggccg caacaccttt
     3601 tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc atccttatgc
     3661 tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg aataggcgca
     3721 tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt tgcatgaagc
     3781 gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc ttttggcgga
     3841 cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg ggccatatgc
     3901 acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt gtgcacacgg
     3961 aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg caccgccatg
     4021 tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc agcggtcata
     4081 tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa agattcgact
     4141 aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt gtctttatcc
     4201 agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta atgaagcaac
     4261 gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag gtcgcgcggt
     4321 ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat atcctgcggt
     4381 tcaaaacgcg cttcttcttc aatctcagcg ttgatggcaa cctgctcgcc acccaatact
     4441 ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc gaggatccat
     4501 tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg atctaagcgt
     4561 tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc catatatact
     4621 gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg tgctattgta
     4681 gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg aggaaagtca
     4741 aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc tgtttttggc
     4801 gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa tttacgcgac
     4861 tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac tggaagcgtt
     4921 agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc tcatctacgc
     4981 ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt ttattcgcct
     5041 taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga ccaatatggc
     5101 gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc gcgatcctca
     5161 acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc caaacagtca
     5221 gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg cgaaatatga
     5281 atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg ttaaccgcgt
     5341 agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc tgccgctgat
     5401 ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag cgaaaatcat
     5461 cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca gcccttgagc
     5521 tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta tcgatctcat
     5581 caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc tgcgatttct
     5641 cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc acacattttg
     5701 acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa aatttatgac
     5761 aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac atgtcgcaga
     5821 ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata tcattctgtc
     5881 caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg gcgttctggt
     5941 tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca acaagctgga
     6001 acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa catcggtgaa
     6061 agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga gtgtatcgcc
     6121 aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt actgtactaa
     6181 agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc ttttttacct
     6241 tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa caataaggcc
     6301 tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga catcggaaaa
     6361 cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat tagcattact
     6421 ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct cgcatcgccc
     6481 ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc tcggtaaagc
     6541 gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg aagattccgt
     6601 attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgt actcagcacg
     6661 catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc agtatgctgc
     6721 ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca
     6781 ggtggaaacc ggccaggcc
//
LOCUS       DAEVZA010000196         6771 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEVZA010000196 DAEVZA010000000
VERSION     DAEVZA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.196"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1479
                     /locus_tag="LAF92_004686"
     CDS             <1..1479
                     /locus_tag="LAF92_004686"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBI9793579.1"
                     /translation="NVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGA
                     GDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVNKGDALTLVELTPAQHFTKPPA
                     RFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEEN
                     FRELMNYDFTAQMENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQ
                     MVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGE
                     DAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGP
                     IVECEKCGSEMHLKMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEK
                     SDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPE
                     GNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK"
     gene            1689..2663
                     /gene="cysB"
                     /locus_tag="LAF92_004687"
     CDS             1689..2663
                     /gene="cysB"
                     /locus_tag="LAF92_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBI9793580.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            2958..3122
                     /locus_tag="LAF92_004688"
     CDS             2958..3122
                     /locus_tag="LAF92_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBI9793581.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            3125..3292
                     /locus_tag="LAF92_004689"
     CDS             3125..3292
                     /locus_tag="LAF92_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793582.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            3406..3501
                     /locus_tag="LAF92_004690"
     CDS             3406..3501
                     /locus_tag="LAF92_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793583.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            3665..6340
                     /gene="acnA"
                     /locus_tag="LAF92_004691"
     CDS             3665..6340
                     /gene="acnA"
                     /locus_tag="LAF92_004691"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBI9793584.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(6404..>6771)
                     /gene="ribA"
                     /locus_tag="LAF92_004692"
     CDS             complement(6404..>6771)
                     /gene="ribA"
                     /locus_tag="LAF92_004692"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase II RibA"
                     /protein_id="HBI9793585.1"
                     /translation="TQIAEEGRGILLYHRQEGRNIGLLNKIRAYALQDQGYDTVEANH
                     QLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTEAGINIVERVPLIVGRNP
                     NNEHYLDTKAEKMGHLLNK"
BASE COUNT         1743 a         1591 c         1734 g         1703 t
ORIGIN      
        1 aatgtgatgg cggaatcgct gaaggatatg gaagcagatg cgcaaaaact gtaccagtta
       61 atctggcgtc agttcgtagc ctgccagatg accccagcga aatatgactc cacgacgctg
      121 accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg ttttgatggc
      181 tggacaaaag tgatgcctgc gttgcgtaaa ggcgatgaag atcgcatctt accagcagtt
      241 aataaaggcg atgctctgac gctcgttgaa cttacaccag cccagcactt taccaagccg
      301 ccagcccgtt tcagtgaagc atcgctggtt aaagagctgg aaaaacgtgg tatcggtcgt
      361 ccgtctacct atgcgtcgat catttcgacc attcaggatc gtggctacgt gcgagtagaa
      421 aatcgtcgtt tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct tgaagaaaat
      481 ttccgcgagt taatgaacta cgactttacc gcgcagatgg aaaacagcct cgaccaggtg
      541 gcaaatcacg aagcagagtg gaaagctgta ctggatcact tcttctcgga tttcacccag
      601 cagttagata aagctgaaaa agatccggaa gagggtggta tgcgcccgaa ccagatggtt
      661 ctgaccagca ttgactgccc gacttgtggt cgcaaaatgg ggattcgcac agcgagcacc
      721 ggggtattcc ttggctgttc tggctatgcg ctgccgccga aagagcgttg caaaaccacc
      781 attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga tgctgaaacc
      841 aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca cggcgatgga cagctatctc
      901 atcgatccga aacgtaagtt gcatgtctgt ggtaataacc caacctgcga cggttacgag
      961 atcgaagagg gcgaattccg cattaaaggt tatgacggcc cgatcgttga gtgtgaaaaa
     1021 tgtggctctg aaatgcacct gaaaatgggg cgtttcggta aatacatggc ctgcaccaac
     1081 gaagagtgta aaaacacacg taagatttta cgtaacggcg aagtggcacc accgaaagaa
     1141 gatccggtgc cattacctga gctgccgtgc gaaaaatcag atgcttattt cgtgctgcgt
     1201 gacggtgctg ccggtgtgtt cctggctgcc aacactttcc cgaaatcgcg tgaaacgcgt
     1261 gcgccactgg tggaagagct ttatcgcttc cgcgaccgtc tgccggaaaa actgcgttat
     1321 ctggccgatg cgccacagca ggatccggaa ggtaataaga ccatggttcg ctttagccgt
     1381 aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa aggcgactgg ctggtcagca
     1441 ttttatgttg atggcaaatg ggttgaagga aaaaaataac ctttaattct gtcaggtttt
     1501 tataaacaaa gggtcgcgaa agcggccctt ttttattgca tattattttt tcttcaaacc
     1561 tatacactaa ggctataaat gatatagtgg ttatagttag cacctttttt attattaaat
     1621 cgtattagtc acccgccagg tgtgacgaaa aaacgatgtt ctgatggcgt ctaagtggat
     1681 ggtttaacat gaaattacaa caacttcgct atattgttga ggtggtcaat cataacctga
     1741 atgtctcatc aacagcggaa ggactttaca catcacaacc cgggatcagt aaacaagtca
     1801 gaatgctgga agacgagcta ggcattcaaa ttttttcccg aagcggcaag cacctgacgc
     1861 aggtaacgcc agcaggacaa gaaataattc gtatcgctcg cgaagtcctg tcgaaagtcg
     1921 atgccataaa atcggttgcc ggtgagcaca cctggccgga taaaggctcg ctgtatatcg
     1981 ccaccacgca tacccaggca cgctacgcgt taccaaacgt tatcaaaggc tttattgagc
     2041 gttatccccg cgtttctttg catatgcacc agggctcgcc gacacaaatt gctgatgccg
     2101 tctctaaagg caatgctgat ttcgctatcg ccacagaagc gctgcatctg tatgaagatt
     2161 tagtgatgtt accgtgctac cactggaatc gggctattgt agtcactccg gatcacccgc
     2221 tggcaggcaa aaaagccatt accattgaag aactggcgca atatccgttg gtgacatata
     2281 ccttcggctt taccggacgt tcagaactgg atactgcctt taaccgcgca gggttaacgc
     2341 cgcgtatcgt cttcacggca acggatgctg acgtcattaa aacttacgtc cggttagggc
     2401 tgggggtagg ggtcattgcc agcatggcgg tggatccggt cgccgatccc gaccttgtgc
     2461 gtgttgatgc tcacgatatc ttcagccaca gtacaaccaa aattggtttt cgccgtagta
     2521 ctttcttgcg cagttatatg tatgatttca ttcagcgttt tgcaccgcat ttaacgcgtg
     2581 atgtcgttga tgcggctgtc gcattgcgct ctaatgaaga aattgaggtc atgtttaaag
     2641 atataaaact gccggaaaaa taatttcttg cgttattttc ggcacctttt atgtagcgaa
     2701 ggtgccggaa tatattctct ttttttactt atttctatac cgtgcacaat atttttatcc
     2761 ctcatattta tagggtaaat tacctgtaaa gtagtgtcta attacccgcc tcgcaaactc
     2821 cagttttcaa atatctattt caggtcaaaa gattgaatta ttcactggag acgattcgta
     2881 aattcgctgg atttttgact aaagtttctt taggatttgt ctcatcgtat gattaattac
     2941 accaatcgtt caacgtcttg ctgataagtg atggcgatta tatgaggtta gcaatgcctt
     3001 ctggaaatca ggaaccccgc cgcgatcctg aattaaaacg taaagcctgg ctggcggttt
     3061 ttcttggttc tgcacttttc tgggtggttg tcgcactgct gatttggaaa gtgtggggat
     3121 aactatggtc ggtcaggagc aactggagtc gtcaccatta tgccagcata gtgacaatga
     3181 aacagaaacg aaacgggaat gttccgtcgt tattccagac gactggcaac taacatcgca
     3241 gcagcaagcc tttatagaac tgtttgctga agatgatcag ccgaaacaat aattatcatc
     3301 attcttatta cccattttta atgaattaaa gggcttttaa tacaccgcag caataacagc
     3361 ttgagttatc tcaacacaaa ataataaccg ttaagggtgt agcctatgat caacacaaat
     3421 atgaaatatt ggtcctggat gggcgcgttt tctctgtcga tgctcttctg ggccgaactc
     3481 ctctggatca ttactcactg atccttgacc ccgctgcggc ggggttgtca tttgctttgc
     3541 cacaaggttt ctcctctttt atcaatttgg gttgttatca aatcgttacg cgatgtttgt
     3601 gttatcttta atattcaccc tgaagagaat cagggcttcg caaccctgtc attaaggagg
     3661 agctatgtcg tcaaccctac gagaagccag taaggacacg ttgcaggcca aagataaaac
     3721 ttaccactac tacagcctgc cgcttgctgc taaatcactg ggcgatatca cccgtctgcc
     3781 caagtcactc aaagttttgc tcgaaaacct gctgcgctgg caggatggta actcggttac
     3841 cgaagaggat atccacgcgc tggcaggatg gctgaaaaat gcccatgctg accgtgaaat
     3901 tgcctaccgc ccggcaaggg tgctgatgca ggactttacc ggcgtacctg ccgttgttga
     3961 tctggcggca atgcgcgaag cggttaaacg cctcggcggc gatactgcaa aggttaaccc
     4021 gctctcaccg gtcgacctgg tcattgacca ctcggtgacc gtcgatcgtt ttggtgatga
     4081 tgaggcattt gaagaaaacg tacgcctgga aatggagcgc aaccacgaac gttatgtgtt
     4141 cctgaaatgg ggaaagcaag cgttcagtcg gtttagcgtc gtgccgccag gcacaggcat
     4201 ttgccatcag gttaacctcg aatatctcgg caaagcagtg tggagtgaat tgcaggacgg
     4261 tgaatggatt gcttatccgg atacactcgt tggtactgac tcgcacacca ctatgatcaa
     4321 cggccttggc gtgctggggt ggggcgtggg tgggatcgaa gcagaagccg caatgttagg
     4381 ccagccggtt tccatgctta tcccggacgt agtgggcttt aaacttaccg gtaaattacg
     4441 tgaaggtatt accgccacag acctggttct cactgttacc caaatgctgc gcaaacatgg
     4501 cgtggtgggg aaattcgtcg aattttatgg tgatggtctg gattcactac cgctggcgga
     4561 tcgcgccacc attgccaata tgtcgccaga atatggtgcc acctgtggct tcttcccaat
     4621 cgatgctgta accctcgatt acatgcgttt aagcgggcgc agcgaagatc aggttgagtt
     4681 ggtcgaaaaa tatgccaaag cgcagggcat gtggcgtaac ccgggcgatg aaccgatttt
     4741 taccagtacg ttagaactgg atatgaatga cgttgaagcg agcctggcag ggccaaaacg
     4801 cccacaggat cgcgttgcac tgcccgatgt accaaaagca tttgccgcca gtaacgaact
     4861 ggaagtgaat gccacgcata aagatcgcca gccggtcgat tatgttatga acggacatca
     4921 gtatcagtta cctgatggcg ctgtggtcat tgctgcgata acctcgtgca ccaacacctc
     4981 taacccaagt gtgctgatgg ctgcaggctt gctggcgaaa aaagccgtaa ctcttggcct
     5041 caagcggcaa ccgtgggtca aagcgtcgct ggcaccaggc tcgaaagttg tttctgatta
     5101 tctggcaaaa gcgaaattga caccgtatct cgacgaactg gggtttaacc ttgtgggata
     5161 cggttgtacc acctgtattg gtaactctgg gccgctgccc gatcctatcg aaacggcaat
     5221 caaaaaaggc gatttaaccg tcggtgcagt gctgtccggc aaccgtaact ttgaaggccg
     5281 tatccatccg ctggttaaaa ccaactggct ggcctcgccg ccgctggtgg ttgcctatgc
     5341 gctggcggga aatatgaata tcaacctggc ttctgagcct atcggccatg atcgtaaagg
     5401 cgatccggta tatctgaaag atatctggcc atcggcacaa gaaattgccc gtgcggtaga
     5461 acaagtctcc acagaaatgt tccgcaaaga gtacgcagaa gtttttgaag gcacagcaga
     5521 gtggaaggaa attaacgtca cacgatccga tacctacggt tggcaggagg actcaaccta
     5581 tattcgctta tcgcctttct ttgatgaaat gcaggcaaca ccagcaccag tggaagatat
     5641 tcacggtgcg cggatcctcg caatgctggg ggattcagtc accactgacc atatctctcc
     5701 ggcgggcagt attaagcccg acagcccagc gggtcgatat ctacaaggtc ggggtgttga
     5761 gcgaaaagac tttaactcct acggttcgcg gcgtggtaac catgaagtga tgatgcgcgg
     5821 caccttcgcc aatattcgca tccgtaatga aatggtgcct ggcgttgaag gggggatgac
     5881 gcggcattta cctgacagcg acgtagtctc tatttatgat gctgcgatgc gctataagca
     5941 ggaacaaacg ccgctggcgg tgattgccgg gaaagagtat ggatcaggct caagccgtga
     6001 ctgggcggca aaaggtcctc gtctgcttgg aattcgtgtg gtgattgccg aatcgtttga
     6061 acgaattcac cgttcgaatt taattggcat gggcatcctg ccgctggaat ttccgcaagg
     6121 cgtaacgcgt aaaacgttag gactaaccgg ggaagagaag attgatattg gcgatctgca
     6181 aaatctacaa cccggtgcga cggttccggt gacgcttacg cgcgcggatg gtagccagga
     6241 ggtcgtaccc tgccgttgtc gtatcgacac cgcgacggag ttgacctact accagaacga
     6301 cggcattttg cattatgtca ttcgtaatat gttgaagtaa caagtatttg cttgccggtt
     6361 attttgcttc cggcaagcaa actaattaca caatgcaaga gggttatttg ttcagcaaat
     6421 ggcccatttt ctcggctttg gtatcgagat aatgttcgtt attggggtta cgacctacaa
     6481 tcaatggtac gcgttcaaca atattaatcc ctgcttcggt cagaatttcg acttttttcg
     6541 ggttattggt taacaagcgg acttcattga cgccaaggag tttgaacata tcagcgcaaa
     6601 gagtgaagtc gcgctcatca gcggcgaagc ctaactggtg gttagcctct acggtatcgt
     6661 aaccttgatc ctgcagtgcg taagcgcgga ttttattcag cagaccaatg ttacgacctt
     6721 cctgacggtg atacagcaaa ataccacggc cttcctcggc aatttgcgtc a
//
LOCUS       DAEVZA010000197         6668 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEVZA010000197 DAEVZA010000000
VERSION     DAEVZA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.197"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1..326
                     /locus_tag="LAF92_004693"
                     /pseudo
     CDS             1..326
                     /locus_tag="LAF92_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            477..986
                     /locus_tag="LAF92_004694"
     CDS             477..986
                     /locus_tag="LAF92_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBI9793586.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            983..2401
                     /gene="phrB"
                     /locus_tag="LAF92_004695"
     CDS             983..2401
                     /gene="phrB"
                     /locus_tag="LAF92_004695"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBI9793587.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGDYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(2443..3924)
                     /gene="dtpD"
                     /locus_tag="LAF92_004696"
     CDS             complement(2443..3924)
                     /gene="dtpD"
                     /locus_tag="LAF92_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBI9793588.1"
                     /translation="MNKLASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            4195..4938
                     /gene="ybgI"
                     /locus_tag="LAF92_004697"
     CDS             4195..4938
                     /gene="ybgI"
                     /locus_tag="LAF92_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBI9793589.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLVADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4961..5617
                     /gene="pxpB"
                     /locus_tag="LAF92_004698"
     CDS             4961..5617
                     /gene="pxpB"
                     /locus_tag="LAF92_004698"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBI9793590.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5611..6543
                     /gene="pxpC"
                     /locus_tag="LAF92_004699"
     CDS             5611..6543
                     /gene="pxpC"
                     /locus_tag="LAF92_004699"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBI9793591.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6533..>6668
                     /locus_tag="LAF92_004700"
     CDS             6533..>6668
                     /locus_tag="LAF92_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBI9793592.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     R"
BASE COUNT         1631 a         1622 c         1854 g         1561 t
ORIGIN      
        1 cggataagaa atccaatgag atcacagcta tccctgaact tcttaacatg ctggatatta
       61 aaggaaaaat catcacaacc gatgcgatgg gttgccagaa agatattgca gagaagatac
      121 aaaaacaggg aggtgattat ttattcgctg taaaaggaaa ccaggggcgg ctaaataaag
      181 ccttcgagga aaaatttccg ctgaaagaat taaataatcc caagcatgac agttacgcaa
      241 ttagtgaaaa gagtcacggc agagaagaaa cccgtcttca tattgtttgc gatgtccctg
      301 atgagcttat tgatttcacg tttgaataga aagggcagct atggttagaa actacctgac
      361 gtcagtcctt gcggggagca ggctttcgta aatttgtcct gctacaacag gattaacttc
      421 acaaatatca tttctcaacg tctacactta ctcctgtaaa ccgttcagga gcagtaatga
      481 atcagcaacg atttgatgac agcaccctaa tccgtatctt cgcccttcat gagttacatc
      541 gactgaaaga acatggctta acgcgcgggg cgcttctcga ttatcacagc cgctataaac
      601 tcgtctttct ggcgcattct cagccggagt accgcaaact tggcccgttc gtggctgata
      661 ttcaccagtg gcaaaatctg gatgattttt acaaccagta ctaccaacgc gtaattgttt
      721 tgctttctca tcccgccaac ccgcgcgatc acaccaacgt tttgatgcac gttcagggtt
      781 attttcgccc gcatattgat tccacagaac gccagcagtt ggcggcgctt atcgacagtt
      841 atcgccgtgg cgagcaacca cttcttgcgc cgctgatgcg tatcaaacac tatatggcgc
      901 tttatcctga cgcctggctt tcagggcagc gttatttcga actttggccg cgtgtgatta
      961 acttgcgcca ttcaggagtt ttatgattac ccatctggtc tggtttcgcc aggatttacg
     1021 tctgcacgat aatctcgcac tggctgccgc ctgccgcaat tcgtctgcac gcgtgctggc
     1081 gttatatatc gctacaccac gccagtgggc gacgcataac atgtcgccgc gtcaggctga
     1141 acttatcaat gctcaactga atgggctaca aatagcgctt gcggaaaaag gtattccttt
     1201 attgttccgt gaagtggatg actttgtcgc cagtgtcgaa atagttaaac aggtgtgcgc
     1261 ggaaaacagc gttacccacc tgttttataa ctatcagtat gaagtgaatg agcgggcgcg
     1321 ggatgtggaa gttgaaagag cgctgcgtaa cgtggtgtgt gaaggatttg atgacagcgt
     1381 gatcctgccg cctggcgcgg tgatgaccgg caatcacgag atgtacaaag tctttacgcc
     1441 ttttaagaat gcctggctga aacggctgcg ggaagggatg ccggagtgcg tcgctgcacc
     1501 aaaagttcgt agtagcggat cgatagagcc ctcgccatcc attacgctga attatcctcg
     1561 tcagtctttc gatactgcgc attttccggt ggaagaaaaa gcggcgattg cgcaattacg
     1621 ccagttttgc cagaacggtg ccggagatta tgagcaacaa cgagattttc cggcagtgga
     1681 aggcaccagc cgtttgtcgg ccagcctggc aacgggcggg ttatcgcctc gccagtgctt
     1741 gcatcgcttg ttggctgaac agccgcaggc gctggacggt ggggccggta gtgtctggct
     1801 tagtgagctg atctggcgcg agttctaccg tcatctgatg acgtattacc cctcgttgtg
     1861 taaacatcgt ccatttattg cctggacgga tcgtgtgcag tggcaaagca atcccgcaca
     1921 tttacaggcc tggcagaaag gcaaaacggg atacccgatt gttgatgccg ctatgcgtca
     1981 gcttaacagc actggctgga tgcataaccg gctacggatg attacagcca gttttctggt
     2041 gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat ttcatgtcgc agctgattga
     2101 tggtgatttg gcagctaata acggtggctg gcagtgggcg gcttctacgg gtactgatgc
     2161 tgcgccgtat tttcgtattt tcaatccgat aactcagggc gagaaatttg accgtgaggg
     2221 cgagtttatt cgtcgatggt taccggagct gcgcgatgta ccagggaaag cggtgcatga
     2281 gccgtggaag tgggcgcaga aagcaggtgt gaagctggat tatccgcaac cgatagttga
     2341 tcacaaagag gcaaggctgc gaacgctggc agcgtacgaa gaagcgagga aaggagcctg
     2401 ataaaacgca gcgagcgttt tatcaggtat ggtgctggcc tattatgact ccagcgccag
     2461 cgcgcggttt ctgaatttca gcgcctgata cagccaaatc atcagtacca gtcctacaca
     2521 cgccagtgcg ccccaggtaa tttgatcaaa tacttcaata tatgcattga tggagtagtt
     2581 gatcgccccg gaagcatcaa acgaagcctg cgatgtctga tcggcaatca cgcccgccag
     2641 atagttcgcg atcgcgccag aaagcagcat gtagatgccg gttaatacgc cggtcacgcc
     2701 cggaatttca atgcgcgtaa tttgcgacat ggcaaccggg tcgataaaca gttccgcaaa
     2761 gcccatcacc gccaggccta ataccatcag tggcagagaa gagtgaccat acattgcgga
     2821 ccagcgggcg cttaaggtca gaatgcagaa cccggcgctc atcaggccga gaccaagagc
     2881 aaattttccc cagatgcgca cggtacgatt acctgcgacg ctttctttaa ccacccacgc
     2941 caggaacaca ccgcacagca taactgcgaa ggcattaatc gactggaaca tagcagtcgg
     3001 aacggtataa ccaaacatat cgcggttaac gaagcggtcg atataaaggc taatcgagct
     3061 gccgccctgt tgtgcgaagg cccagaacaa catactgaag aaggtgagcg tcacaatcag
     3121 ccccagctcc ttacgctgtt tctggttttc tgctttgcga taaatttttg ccagtacacc
     3181 caggccaata atagtcgcga caattaaggc gtataccgac cactctttcc agaacagtac
     3241 ggtaatcagc gcaggcgttg ccaccagcag aaccagcagc catccccagt taggcaggag
     3301 aaagtttgtt gcacgcagta cttttttatt aacgccgcgg gtatgtgtga aatgacgatt
     3361 gccacataag aaaatgacca gacccgcgat catacccacc gccgccaggc caaagcccat
     3421 cgcccagctg tactcttcct gggcgtaacc acaggcgata ggtgcgataa tagaccccac
     3481 gttacccgct gcatacatca gcgagaaacc gccatcacga cgcggatcgg ttggctcata
     3541 cagctcgccg agcaggcaac tgacattgga tttaaacaga ccatagccac aaacgataat
     3601 cgccagggac agatagagga atgacggatg gatctcactg gcacccagca ccacatgacc
     3661 gatcgccatc aacaacgccc ccagcatcac cgccatgcga ttgccgagaa ctttatccgc
     3721 caaaaagcca ccgaggattg gcgtgacata caccagcgaa cagtaggcgc taaataactc
     3781 gtaggcgtga gtatcgttgt attttagttg attggtgaga tagagaatca gcagggcacg
     3841 catgccgtaa aagctaaaat attcccagat ttgcagcgca acgacatagt aaatagcgcg
     3901 cggttgtgat gcgagtttat tcataataat tccacaattg acggcaacgt cacgtaatag
     3961 ttgcgcggcg tgcttgtgtt tccttaaagt taaaactttg atacagatct gattatttat
     4021 gcaatatgct gtctgattgc ataaatatac attagctgaa gcgtgatgat ataaacaatt
     4081 tgcgtctttt ggcgtcgggt ttgtttctta atatgtcaaa agattggcta tcgacgaaca
     4141 ggcgaggtaa cgctatgtta acggtgtagc tatcagaata cggaaggcgc gaagatgaaa
     4201 aacaccgaac tggaacaact gattaacgaa aaactgaaca gcgcggcgat tagtgattac
     4261 gcgccgaatg gtttgcaggt ggaaggcaaa gagacggtgc aaaaaattgt taccggtgtc
     4321 accgccagcc aggctttgct cgatgaggca gtgcgtctgg tcgcggatgc ggtcattgtg
     4381 catcatggct acttctggaa aggagagtct ccggtcattc gcggaatgaa gcgtaaccgt
     4441 ttaaaaacgt tgctggcgaa tgatatcaac ctgtatggct ggcatttgcc gcttgacgca
     4501 catcctgagc tgggcaataa cgcacagctg gcggcgttac tggggatcac ggtcatgggc
     4561 gaaattgagc cgttggtgcc gtggggagag ctgaccatgc ctgtgccggg actggaacta
     4621 gcctcctgga ttgaagcgcg tctgggacgt aagccgttat ggtgtggcga taccggacct
     4681 gaggtggtcc agcgcgtcgc ctggtgcacg ggcggcgggc aaagttttat cgatagcgcc
     4741 gcgcgttttg gcgtggatgc ttttattact ggcgaagttt ctgaacagac cattcattca
     4801 gcccgcgagc aggggttgca tttttatgct gcaggtcacc atgccactga acgtggtggt
     4861 attcgcgcat tgagcgagtg gctgaatgaa aataccgatc ttgatgtgac ctttattgat
     4921 attcctaatc ctgcataacg aataatcaga gggatcgaaa gtgcaacgag cgcgttgtta
     4981 tctgataggt gaaacggcgg tagtgctgga actggaaccg ccggtgacgc tggctagcca
     5041 gaaacggatc tggcgactgg cgcagcgtct ggtggatatg ccgaatgtgg ttgaagccat
     5101 tcccggcatg aacaatatca cggtgatttt gcgtaatcct gagtcgctgg cgctggatgc
     5161 catagagcgt ttgcaacgct ggtgggagga gagcgaggcc ctggaacctg agtctcgctt
     5221 tattgaaatt ccggtggttt acggtggtgc aggcggaccg gatttggcgg tggtcgcggc
     5281 gcattgcggg ttgagcgaaa aacaggttgt tgaattgcac tcctccgtgg aatacgtggt
     5341 ctggttttta ggttttcaac cgggcttccc gtatctcggg agtttgccgg aacaactaca
     5401 cacgccacgg cgcgctgaac cgcgcttact cgttccggca ggttctgtcg ggatcggcgg
     5461 gccgcagact ggtgtttatc cgctggcaac gccgggtggc tggcagttga ttggtcatac
     5521 ctcactcagc ctgtttgatc cggcgcgtga cgaacccatc ttattacgtc cgggagacag
     5581 cgtgcgcttt gtaccgcaga aggagggagt atgctgaaga ttattcgtgc gggcatgtat
     5641 accactgtgc aggatggcgg tcgtcacggt tttcgccagt cgggtatcag ccactgcggc
     5701 gcactggata tgcccgcgct gcgcattgct aacctgctgg tgggtaatga cgccaatgcc
     5761 cccgcgctgg agatcacgct cggtcagtta acggttgagt tcgaaactga tgggtggttt
     5821 gctctgacag gggccggttg cgaagcgcgg ctggatgata atgccgtctg gaccggctgg
     5881 cgattgccga tgaaagcagg ccagcgttta acgcttaaac gcccgcagca cgggatgcgc
     5941 agttatctgg cggtcgcggg tggtattgat gttccgccgg taatggggtc atgcagcacc
     6001 gatctcaacg tggggattgg cgggctggaa ggccgtttac tgaaggatgg tgaccgactc
     6061 ccgattggca aatcgaagcg tgattttatg gaagcgcagg gcgttaaaca gctgctgtgg
     6121 ggcaaccgca ttcgcgcctt gccggggccg gaatatcatg agttcgatcg cgcctcgcag
     6181 gatgcattct ggcgttcgcc ctggcagctt agcccgcaaa gtaaccgcat gggctatcgc
     6241 ttacaggggc aaattttaaa acgcaccacc gatcgcgaac tgttatctca cggtttgtta
     6301 ccgggtgtgg tgcaggtgcc gcataacggg cagcctattg tgttgatgaa cgacgcacag
     6361 accaccggtg gttacccgcg tattgcctgt atcattgagg ctgatatgta ccatctggcg
     6421 caaattccgc tcggtcagcc gattcatttt gtccagtgtt cactggaaga ggcactgaaa
     6481 gcgcggcaag atcaacaacg ttatttcgaa caattagcgt ggcggctaca caatgaaaat
     6541 tgacctgaac gccgatctgg gcgaaggctg cgccagcgac gcagagctat taacgctggt
     6601 ttcctctgcc aatattgctt gtggatttca tgcaggcgat gcccaaacca tgcaggcttg
     6661 cgtgcgtg
//
LOCUS       DAEVZA010000198         6664 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEVZA010000198 DAEVZA010000000
VERSION     DAEVZA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.198"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            5..520
                     /locus_tag="LAF92_004701"
     CDS             5..520
                     /locus_tag="LAF92_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI9793593.1"
                     /translation="MIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDSLNFTV
                     DLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLKLDEGS
                     NTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLV
                     RASATFTLEFQ"
     gene            533..1036
                     /locus_tag="LAF92_004702"
     CDS             533..1036
                     /locus_tag="LAF92_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI9793594.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            1096..2010
                     /locus_tag="LAF92_004703"
     CDS             1096..2010
                     /locus_tag="LAF92_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI9793595.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
     gene            2344..4623
                     /gene="ydeP"
                     /locus_tag="LAF92_004704"
     CDS             2344..4623
                     /gene="ydeP"
                     /locus_tag="LAF92_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBI9793596.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGKRYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4871..5068
                     /gene="safA"
                     /locus_tag="LAF92_004705"
     CDS             4871..5068
                     /gene="safA"
                     /locus_tag="LAF92_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBI9793597.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            5142..5903
                     /gene="ydeO"
                     /locus_tag="LAF92_004706"
     CDS             5142..5903
                     /gene="ydeO"
                     /locus_tag="LAF92_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBI9793598.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
     gene            6307..>6664
                     /locus_tag="LAF92_004707"
     CDS             6307..>6664
                     /locus_tag="LAF92_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBI9793599.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEG"
BASE COUNT         1916 a         1417 c         1486 g         1845 t
ORIGIN      
        1 taacattatt ttcctcggtt tatgtctggg gttaaccacc tattctgctt tatccgcaga
       61 tagcgttatt aaaattagcg ggcgcgtcct cgattatggc tgcacagtct catcggattc
      121 gcttaatttt accgtagatc tccaaaaaaa cagtgccaga caatttccaa cgaccggtag
      181 cacaagtcca gccgtccctt ttcagattac gttaagtgaa tgcagcaaag ggacaacggg
      241 ggttcgggtt gcatttaacg gtattgagga tgcagaaaat aatactttgt tgaaactgga
      301 tgaaggaagc aatacggcct ccggtttggg tatagaaata ttggacggaa atatgcgtcc
      361 ggtgaaactg aatgaccttc atgccgggat gcagtggatc ccactggtac cagaacagaa
      421 caatattttg ccttactccg ctcgtctgaa gtcaactcag aagtccgtca atccgggact
      481 ggtgagggct tcggcaacct ttacccttga atttcaataa tcagagtcag atatgaaaag
      541 acttcacaag aggttcctgt tagctacgtt ttgcgcgtta ttcacagcaa ctctccaggc
      601 cgccgatgtc actatcactg ttaatggtcg ggtagtcgct aaaccctgca ctattcaaac
      661 caaagaagct aacgttaatc tcggggatct ttatacgcgc aatctgcaac aacctggttc
      721 tgcatctggc tggcacaata ttactttgtc attaaccgat tgtccggttg aaacaagtgc
      781 agtgacggca atcgtgacag gttcaactga caatacgggt tattacaaaa atgaaggtac
      841 tgccgaaaat attcagatag agctgaggga tgaccaggat gcgacgttaa aaaatggcga
      901 tagcaaaacg gttattgttg atgagatcac tcgtaatgca cagtttccac ttaaggcaag
      961 agctatcacg gtgaatggaa acgcaagcca gggaacgatc gaggcgctaa tcaatgtgat
     1021 ctacacctgg caataacgtc gattcgattt tcttagcctt ctttttaaat caatttttta
     1081 accttaggat atatcatggg taaaacaatc agtataaagg tcctgttcgg tatttatctt
     1141 ctattaatgg caggtaaagt ttttgccttc agttgtaatg tcgatggcgg gtcaagtatt
     1201 ggagctggta caacctctgt ttatgttaat ctcgaccctg taatacagcc gggccagaat
     1261 ctggttgtag acttgtctca gcatataagt tgctggaatg attacggcgg ctggtacgac
     1321 actgatcata taaacctggt acaaggctca gctttcgcag gctctctaca gtcatataaa
     1381 ggatcgctgt actggaataa tgtgacctac cccttcccgt tgacgacaaa taccaacgta
     1441 ttggatattg gtgataagac accgatgcca ttaccgttaa aactctatat taccccagtt
     1501 ggggcggccg gtggagttgt cataaaagcg ggggaagtca ttgcccgtat tcatatgtat
     1561 aaaattgcaa cattaggaag cggaaatccg cgtaatttta cgtggaatat tatttcaaat
     1621 aacaatgttg ttatgccaac aggcggctgc accgttgata gtcgtaatgt caccgtggat
     1681 ctcccggact ttcctggaag cgcagaaata ccccttggcg tatattgctc aagcgaacaa
     1741 aagctctcat tttatctttc gggtgccacc actgacagta gccgacaagt gtttgctaac
     1801 acagcacctg atgcaacaaa agcatcggga gtgggtgtca ctctaatgcg taatggaaaa
     1861 atactggcta cgggagaaaa tgtctccctg gggacagtaa ataaaagcaa agtacctctg
     1921 ggtttaagcg caacctatgg acaaacagga aacaaggttg cagccggaac cgtccagtca
     1981 gtcattggtg tcacttttat ctatgaataa ttgtgatttt gaccttagtc tatgcgctcc
     2041 attcacgaaa attgcgcgga taattatttt gtgaaggcta ttagcctaca cctgtaagaa
     2101 aatccgcgca caattgaagc cagagtctta ttttttatat tgaaaaatat gttaatttaa
     2161 acactatgag agatgatgct taacttatcg tatttaatct attgtttaac gataatagat
     2221 taaaaataga aagagagtag tcccctactc ttatatatcc atgttggcga taatcccttt
     2281 gtatgtactg tgcatcatcg ctattacaaa tcctaataat tcatttccac acaggataag
     2341 tagatgaaga aaaaaattga atcctaccag ggtgctgcag gtgggtgggg tgctgtcaaa
     2401 tccgtagcga atgcagtacg taagcagatg gatatacgcc aggatgttat tgccatgttt
     2461 gacatgaata agccagaggg ctttgattgt ccgggttgtg catggccaga tcctaagcac
     2521 agtgcgtcat tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga agtcacggat
     2581 aagcaggtaa atgcctcttt ctttgctgag aatacggttc aatcattact ttcctgggga
     2641 gatcacgagc ttgaggctgc gggacgtctc actcagcctt tgaaatatga tgccgtcagc
     2701 gactgttaca agccattaag ctggcaacaa gctttcgacg aaattggcgc acgccttcaa
     2761 agctatagtg atcccaatca ggttgaattc tatacttcgg gccgcacttc caatgaagct
     2821 gcctttcttt atcagctttt tgcccgtgaa tacgggagca ataactttcc cgactgctcc
     2881 aacatgtgcc atgaaccgac cagtgtgggt ttggcagcga gtatcggtgt aggtaaaggg
     2941 accgtgttgc tggaagactt tgagaagtgc gatttagtca tttgcattgg gcataaccct
     3001 ggtacaaacc accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg gggagcgaaa
     3061 atgatcgcca tcaatcctct acaggaacgt ggcctggagc gatttaccgc accgcaaaac
     3121 ccgtttgaaa tgctgacgaa ctctgagact cagttggcca gtgcctacta caacgtgcgc
     3181 attggtggcg atatggcgtt gctcaagggg atgatgcgcc tgttaattga gcgcgatgat
     3241 gctgcaagtg ccgcaggtcg gccctcattg cttgatgacg aatttattca aacgcatacc
     3301 gtcggctttg acgagctacg ccgtgacgtt ctcaattccg agtggaaaga tatcgaacgt
     3361 atttctggac taagtcagac acaaatcgcc gaactggctg acgcatatgc cgctgccgaa
     3421 cgcaccatta tctgttacgg aatggggatc actcagcacg aacatggtac ccagaacgta
     3481 cagcaactgg tcaatttgct gttgatgaaa ggtaacattg gcaagcctgg tgcgggtatc
     3541 tgcccactac gtggtcactc taatgtacag ggcgaccgaa ccgtcggtat caccgagaaa
     3601 ccgtctgcag agtttctggc tcgcctgggt aagcgctatg gcttcacccc acctcatgca
     3661 cctggacatg ctgcaattgc cagcatgcaa gcaatatgta cggggcaggc tcgagcattg
     3721 atctgcatgg ggggcaattt tgcgctggca atgccagatc gggaagcgag cgctgtaccg
     3781 ttaacgcaat tagatttggc ggtacacgta gccactaagc ttaaccgctc tcatctgctg
     3841 accgcacggc atagctatat tctgccggtt ctggggcgta gcgagattga tatgcaaaaa
     3901 agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga tgattcatgc ctcgcgtggc
     3961 gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg ctgtggtcgc gggaatcgcc
     4021 caggcaacac taccccagag cgtggtagcc tgggagtatc tggtggaaga ttatgatcgc
     4081 attcgcaatg acattgaagc tgtgctgcca gagttcgccg actataacca gcgcatccgt
     4141 catcccggtg gttttcacct gataaatgca gctgctgaaa ggcgctggat gacgccgtca
     4201 ggtaaggcta atttcattac cagcaaaggg ctgttagaag atccctcttc agcgtttaac
     4261 agtaagctgg tcatggcgac agtacgcagc cacgatcagt acaacacgac gatttatggt
     4321 atggatgatc gctatcgagg ggtattcggt caacgagatg tggtctttat gagtgctaaa
     4381 caagctaaaa tttgccgtgt aaaaaacggc gaaagagtta atcttattgc gcttacgcca
     4441 gacggtaagc gcagctcacg ccgcatggat agattaaaag tggtcattta ccctatggct
     4501 gaccgctcac tggtgaccta ttttccagaa tcgaatcaca tgctaactct tgataaccac
     4561 gatccattaa gtggcattcc tggttataaa agtattccgg ttgaattaga accatcaaat
     4621 taattcgcct cctcatttct tctgctgtca tccgcacagc agaagaattt ctcattgact
     4681 attatcgcgc aatttgctca cattgattaa attaaaccac acactataag atataaactt
     4741 ctgcctacag ctgtaagaaa ctccgctcag tactgaagca ccagtcctat ttcctctttt
     4801 ctccagcctg ttatattaag catgctgatt aacgattttt aacgttatcc gctaaataaa
     4861 catatttgaa atgcatgcga ccacagtgaa aaacaaaatc acgcaaagag acaactataa
     4921 agaaatcatg tctgtaattg tggttgtctt attactgaca cttacgttga tagccatttt
     4981 ttcggcaatt gatcagctga gtatttcaga aatgggtcgc attgcaagag atcttacaca
     5041 tttcattatc aatagtttgc aagactgaaa ataaacagca aattataaat gtgaaatgtt
     5101 taaaaggtat cgataaaact ttattgtttt aaggagataa aatgtcgctc gtttgttctg
     5161 ttatatttat tcatcatgcc ttcaacgcta acattttaga taaagattac gccttctctg
     5221 acggcgagat cctgatggta gataacgctg ttcgtacgca ttttgaacct tatgagcggc
     5281 attttaaaga gatcggattt actgaaaata ccattaaaaa atatctacaa tgcactaaca
     5341 tccagacagt gacggtgcct gttcctgcga agtttttacg tgcttcaaat gtaccgactg
     5401 gattgcttaa tgaaatgatt gcttatctca actcggaaga acgcaatcat cataattttt
     5461 cagaactttt gcttttttct tgcctgtcta tttttgccgc atgcaaaggt ttcattacac
     5521 tattaactaa cggtgtgcta tccgtttccg gaaaagtgag aaatattgtc aacatgaagc
     5581 tggcgcaccc atggaagctg aaagatattt gtgactgcct gtacatcagt gaaagcctgt
     5641 tgaagaaaaa acttaagcaa gagcaaacga cattctcaca gattctttta gatgccagaa
     5701 tgcagcacgc aaaaaatttg atacgcgtag aaggttcagt caataaaatt gccgaacaat
     5761 gtggttatgc cagtacatct tattttattt atgcgttccg caaacatttc ggcaacagtc
     5821 cgaagagagt ttctaaggag taccgttgtc aaaggcacac gggtatgaat acgggcaaca
     5881 cgatgaatgc tttagctatt tgattatttg ctaacgagta gtcaaccacg caccctacgt
     5941 aaaaattaaa aatggggcag tcattccctg ccccgcgttg tttttaggcg atatatttat
     6001 tgaaataaat aagtgacatt catcacatat ttatgcattt gcataacctg ttgcatgatt
     6061 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     6121 gattagttgt ttatcggcga gaaattactt agtagaacag aaagtaatgt caacgcttta
     6181 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     6241 ttacacccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     6301 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     6361 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     6421 aaaacaaacg ttgctttctc agactttacg ccgactgaat acagtaccaa aggaaagcca
     6481 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     6541 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     6601 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     6661 ggcg
//
LOCUS       DAEVZA010000199         6460 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEVZA010000199 DAEVZA010000000
VERSION     DAEVZA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.199"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..322
                     /locus_tag="LAF92_004708"
     CDS             <1..322
                     /locus_tag="LAF92_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBI9793600.1"
                     /translation="FNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAY
                     TPYNIHTYKTEEVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSS
                     SWPF"
     gene            646..2034
                     /gene="narU"
                     /locus_tag="LAF92_004709"
     CDS             646..2034
                     /gene="narU"
                     /locus_tag="LAF92_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBI9793601.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGVFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2116..5856
                     /gene="narZ"
                     /locus_tag="LAF92_004710"
     CDS             2116..5856
                     /gene="narZ"
                     /locus_tag="LAF92_004710"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HBI9793602.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLRYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5853..>6460
                     /locus_tag="LAF92_004711"
     CDS             5853..>6460
                     /locus_tag="LAF92_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBI9793603.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKR"
BASE COUNT         1549 a         1689 c         1659 g         1563 t
ORIGIN      
        1 atttaatttg ttttccccag cacttaagct taatactggc ctggcaattc tttcgcctgg
       61 tgcatttgaa gttcactctg acggaataga tgtggataac gaattgtttc actatcctat
      121 taaaaaagca tataccccat ataatataca cacttacaag acagaggaag ttgtaaacca
      181 gaggaatata aaagttaaaa acatgacctt agatgaaata aacaatactt actgtaataa
      241 cgattattac aatcaggcaa taagagagga accgatagac cttctggaca gatcgttttc
      301 ctccagttca tggccttttt agtgaataaa tcatcactaa ttctataagt gtctggtctt
      361 gttgataata tagttaatat gtaacccttt agattcacga tctgaagggt tgaaacatga
      421 catacgttca aagattaacc cccgtttgaa ggttagcttg ttagttacat ttagtaacac
      481 atatctggct acttaatccc cattctgccc ccgcggttac gctttcttat ctattctttc
      541 aatcagcact tcacccaatt catccttaat tctaataact ggctattgcg ccttacctcc
      601 tgttttgtct tttttcatta ccaatgtgtg catgtgagga acaatatggc actgcaaaat
      661 gagaaaaata gtcgttatct tttacgcgac tggaaaccag aaaatccggc cttctgggaa
      721 aataaaggaa aacatattgc tcgaagaaac ctctggatat cagtcagttg tctacttctt
      781 gccttctgtg tctggatgct atttagcgca gttaccgtta atctcaataa aatcggtttt
      841 aattttacta ccgatcaact ctttttatta accgcattac cctccgtttc tggcgcatta
      901 ttgcgtgttc cctactcctt tatggtgcct atattcggtg gacgccgatg gacggttttt
      961 agtactgcaa tcctgattat tccttgcgtc tggctcggaa ttgccgtgca aaatccgaat
     1021 actccttttg gggtatttat cgttatcgct ttgctatgcg gttttgcagg tgcaaacttt
     1081 gcttcgagca tgggcaatat cagtttcttc tttccaaaag ccaaacaagg gagcgctctt
     1141 gggattaatg gcggattagg aaacttaggt gtaagtgtaa tgcagctggt tgcaccgctg
     1201 gtcatttttg tacctgtatt tgcctttctc ggcgtcaatg gcgtaccgca ggccgacggt
     1261 tcggtgatgt cgctggcgaa tgccgcatgg atttgggtac cgctactggc gattgccacg
     1321 atcgccgcct ggtcagggat gaatgatatc gccagttcac gcgcctcaat tgccgaccag
     1381 ctccctgtct tacaacgcct gcatctctgg ctgctgagcc tgctttacct tgccaccttc
     1441 ggttcgttta tcggtttttc tgcgggtttt gccatgctgg caaaaaccca gttcccggat
     1501 gtgaatattc tgcgcctggc gttctttggc ccatttatcg gtgccatcgc gcggtcggtt
     1561 ggtggtgcta tttccgataa gttcggcggc gtgcgggtga cgttgatcaa ctttattttt
     1621 atggcgattt tcagtgccct gctgttcctt accttaccgg gcacaggctc cggtaatttc
     1681 atcgcctttt acgccgtatt tatggggctg tttctgactg cgggtctggg aagtggttct
     1741 actttccaga tgatcgccgt catctttcgc cagataacca tttatcgggt aaagatgaaa
     1801 ggcggtagtg atgagcaagc tcagaaagaa gccgtcaccg aaacggcggc ggctctgggc
     1861 tttatctcag ccattggcgc agtgggcggc ttttttattc cgcaggcgtt tggcatgtcg
     1921 ctcaatatga ccggctctcc ggtcggcgcg atgaaagtgt ttttaatctt ctacatcgtt
     1981 tgtgtgctgc tgacctggct ggtttatggt cggcggaagt tcagccaaaa ataagagatc
     2041 gatgcctgat gtacaaccgt gtcgcatccg acaaggcatc agatcccaat tcattcctgg
     2101 agcaggagtc atgtcatgag taaacttttg gatcgctttc gctacttcaa acaaaagggc
     2161 gaaacctttg ccgatggtca cggacaggtg atgcatagca accgcgactg ggaggacagc
     2221 tatcgccagc gttggcagtt cgacaaaatc gtgcgttcca cccacggtgt taactgtaca
     2281 ggctcctgta gctggaaaat ctacgttaaa aatggtctgg tgacctggga aatccaacag
     2341 accgactacc cgcgcactcg ccctgacctg cccaatcatg aacctcgcgg ctgcccgcgt
     2401 ggcgcaagtt actcctggta tctttacagc gctaaccgcc tgagataccc gctcattcgt
     2461 aaacgactga ttgaactgtg gcgcgaagcc ctcaagcaac acagcgatcc ggtactggcg
     2521 tgggcatcga ttatgaacga tccgcaaaag tgcctgagct acaaacaagt gcgtgggcgc
     2581 ggcgggttta tccgctccaa ctggcaggaa ctaaaccagc tgattgccgc cgctaacgtc
     2641 tggaccatca aaacctacgg cccggatcgc gttgccggtt tctcgccgat cccggcgatg
     2701 tcgatggttt cttacgccgc cggaacgcgt tatctgtcgc tgcttggcgg cacctgttta
     2761 agtttctacg actggtattg cgacctgccg cccgcctcgc cgatgacctg gggcgagcaa
     2821 accgacgtac cggaatctgc cgactggtat aactccagct acatcatcgc ctgggggtct
     2881 aacgtaccgc agacacgtac gccggacgcc cacttcttta ccgaagtacg ctacaaaggc
     2941 actaaaacca tcgccattac ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg
     3001 ctggcaccga aacaaggcac tgatagcgcc ctggcgatgg caatgggcca tgtgatttta
     3061 aaagagtttc atctcgataa tcccagcgac tactttatca actactgccg ccgctacagc
     3121 gacatgccga tgctggtaat gctggagcct cgcgacgatg gtagctacgt tcccgggcgc
     3181 atgatccgcg catctgacct ggtggatgga ctgggcgaaa gcaacaatcc gcagtggaaa
     3241 accgtagcag ttaataccgc aggtgaattg gtagtgccga acggttcgat tggtttccgc
     3301 tggggagaaa aaggcaaatg gaatctggaa tccattgccg ccggtacgga aaccgaattg
     3361 tcgttaaccc tgctcggtca acatgacgct gttgcaggcg tggccttccc ctactttggc
     3421 ggcattgaaa atccgcattt tcgcagcgta aaacacaatc cggtgctggt gcgccaattg
     3481 cccgttaaaa acctgacgtt agtcgatggc aacacctgtc cggtggtcag cgtttatgat
     3541 ttggtactgg cgaattacgg cctcgatcgc gggctggaag atgaaaacag tgcgaaagat
     3601 tacgctgaaa tcaaaccgta caccccagcc tggggtgagc aaattaccgg cgtgccgcgc
     3661 cagtatattg aaaccatcgc ccgtgaattt gccgataccg cccataaaac gcatgggcgc
     3721 tcgatgatta tcctcggcgc aggtgttaac cactggtatc acatggacat gaactaccgc
     3781 gggatgatca atatgctgat cttctgcggt tgtgtcgggc aaagcggtgg cggctgggca
     3841 cactatgtcg gccaggaaaa actgcgccca caaaccggct ggttgccgct ggcctttgcg
     3901 ctcgactgga accgaccacc acgccagatg aacagtacct cgtttttcta caatcattcc
     3961 agccaatggc gctatgaaaa agtctctgct caggagttac tttcaccgct cgccgatgcc
     4021 agtaagtaca gcggtcatct gattgatttc aacgttcgcg ccgaacgtat gggctggcta
     4081 ccttctgcgc cgcagctggg gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc
     4141 ggattatccc ccacagaatt taccgcccag gcgctgaaat cgggcgattt acgtatggcc
     4201 tgcgaacaac cagatagcgg cagcaatcat ccgcgtaatt tgtttgtctg gcgctctaac
     4261 ctgcttggct cctccggcaa aggccacgag tatatgcaga agtatctgct ggggaccgaa
     4321 agcgggattc agggcgagga actcggtgcc agcgacggga tcaaaccgga agaagtcgag
     4381 tggcaaactg cggcgattga aggcaagctc gacctgctgg tgacgctcga cttccgcatg
     4441 tccagtacct gcctgttctc cgatatcgtt ctgcccaccg ccacctggta cgaaaaagac
     4501 gatatgaaca cctcggatat gcatccgttt attcatccgc tttctgcggc ggtcgatccg
     4561 gcgtgggaat cacgcagcga ctgggaaatc tacaaaggta tcgccaaagc attttcgcaa
     4621 gtgtgcgtgg gtcatcttgg caaagaaacc gacgtggtat tacaacccct actgcatgac
     4681 tctccggcag agctctcaca gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat
     4741 ctgattccag gcaaaaccgc gccgaatatt gtggcggtgg agcgcgacta ccctgctacg
     4801 tatgaacgct ttacctcgct cgggccattg atggacaaac ttggcaatgg cggtaaaggg
     4861 atttcgtgga atacccagga tgaaatcgat ttcctcggca aactcaatta caccaagcgt
     4921 gatggcccgg cacaggggcg tccgctgatt gacaccgcca ttgacgcttc agaagtgatt
     4981 ctggcactgg caccggaaac caacggtcat gttgcagtca aagcgtggca ggcgctgggc
     5041 gagatcaccg ggcgcgaaca tacccatctg gcgctgcaca aagaggacga gaagattcgc
     5101 tttcgcgata ttcaggcgca gccgcgtaaa attatctcca gccccacctg gtccggtctg
     5161 gaaagcgatc acgtatccta taacgcggga tacaccaacg ttcatgagtt aattccgtgg
     5221 cgcacgctgt cgggacgcca gcagctctat caggatcatc cgtggatgcg tgcttttggt
     5281 gaaagcctgg tggcttatcg cccgcctatc gacacccgta gcgtcagtga gatgcgccag
     5341 ataccgccaa acggcttccc ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg
     5401 ggcattcact caacctacag tgaaaacctg ctaatgctga cgctctctcg cggtggaccg
     5461 attgtctgga tcagcgaaac agatgcccga gaactgacca ttgtcgataa cgactgggtg
     5521 gaagtattca acgccaacgg cgcgctgact gcccgcgcgg tggtcagcca acgtgtaccg
     5581 ccgggcatga ccatgatgta tcacgcccag gaacgcatta tgaatattcc tggttcggaa
     5641 gtaactggca tgcgcggcgg cattcataac tcggtcaccc gcgtttgccc gaaaccaacg
     5701 catatgattg gcggttacgc gcagctggcc tggggcttta actactacgg caccgtcgga
     5761 tcgaaccgcg atgagttcat catgatccgc aagatgaaga acgttaactg gctggatgat
     5821 gaaggtcgcg atcaggtaca ggaggcgaaa aaatgaaaat ccgttcacaa gtcggcatgg
     5881 tgcttaacct cgacaaatgt atcggctgcc atacctgttc ggtgacctgt aaaaacgtct
     5941 ggaccgggcg cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca
     6001 ttggttatcc gaaaaactgg gaagatcagg aagagtggca aggcggctgg gtccgcgatg
     6061 tgaatggcaa gatccgtccg cgtctgggca gcaagatggg cgtaataacc aaaatcttcg
     6121 ccaacccggt ggtgccgcag attgatgatt actacgaacc tttcaccttc gactacgaac
     6181 atttgcatag cgcaccggaa ggcaaacata tccctactgc tcgcccgcgt tcactgattg
     6241 acggcaaacg gatggacaaa gtgatctggg gcccaaactg ggaagaactg ctgggcggcg
     6301 agtttgaaaa acgtgcccgc gaccgcaact tcgaggccat gcaaaaggag atgtacgggc
     6361 agtttgaaaa caccttcatg atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca
     6421 gttgcgtggc gacctgccca agcggcgcta tctacaaacg
//
LOCUS       DAEVZA010000200         6300 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEVZA010000200 DAEVZA010000000
VERSION     DAEVZA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.200"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="LAF92_004712"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="LAF92_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBI9793604.1"
                     /translation="MVESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="LAF92_004713"
     CDS             complement(1608..1931)
                     /locus_tag="LAF92_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBI9793605.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="LAF92_004714"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="LAF92_004714"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBI9793606.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="LAF92_004715"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="LAF92_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBI9793607.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..>6300)
                     /locus_tag="LAF92_004716"
     CDS             complement(6138..>6300)
                     /locus_tag="LAF92_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HBI9793608.1"
                     /translation="YCTAEVAIALLDMAGDTEAAAGLGELFTRFKTRYLAGKTQHLGS
                     ITAEQLESV"
BASE COUNT         1481 a         1631 c         1596 g         1592 t
ORIGIN      
        1 aaaggccatc agtcacgatg gcctttttgc gttggcgcag aaaaaaatgc ctgatgcgac
       61 gctgcgcgtc ttatactccc acatatgcca gattcagcaa cggatgcggt ttccccaact
      121 tgcctacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcataatg atacgcaaca aatgtataac
      241 attttcttta ccaaaagaaa taaataaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggttga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc gattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tactttgccc acattttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgttatcgg tgtgtatgat gtgtttcgga tcgctggtta tcgcctgcct cccaggttat
      661 gaaacaatag gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tctgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac cacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgccaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcaataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagctccag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttta ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatca
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gatcagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccggattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacggcaaat tcggcatcga tgttatcagg gtcggagatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgcgtcatg ttggctaaat cttcatgagt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgcgatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcag gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca cacctccctc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagcc cggttagcca
     4381 tactttgcac caacaggccg tggatccgcg cctgcggcca tgccattttt acgcgatcga
     4441 aaatagcgtt tgccgcttgc tggacttcat tggctgtacg caggtacagc attacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggtg gggagcgtgt
     4621 tcatgccata cgcttgcagg ataggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgccgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 acgcggtgat ggtgccttcc ggcgtgcgat aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta attcttgcgc gctttctgtt gcgggagccg
     4981 ctgcgctggg tgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgagc gggttagaga
     5101 ttgccacatg tccaggcagt gcatcgcgca gtttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgtgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ctcgtgggtg tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccacgccgg atccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggccgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 caagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggcagccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc tagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcatgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gctaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg gaagtaccgg tccgttaaag cctcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgatgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aaagctcgcc taaccctgct
     6241 gccgcctcgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
//
LOCUS       DAEVZA010000201         6297 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEVZA010000201 DAEVZA010000000
VERSION     DAEVZA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.201"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            87..806
                     /locus_tag="LAF92_004717"
     CDS             87..806
                     /locus_tag="LAF92_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBI9793609.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            982..2028
                     /gene="ansB"
                     /locus_tag="LAF92_004718"
     CDS             982..2028
                     /gene="ansB"
                     /locus_tag="LAF92_004718"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBI9793610.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            2145..3152
                     /locus_tag="LAF92_004719"
     CDS             2145..3152
                     /locus_tag="LAF92_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBI9793611.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(3208..4509)
                     /locus_tag="LAF92_004720"
     CDS             complement(3208..4509)
                     /locus_tag="LAF92_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBI9793612.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(4509..5012)
                     /locus_tag="LAF92_004721"
     CDS             complement(4509..5012)
                     /locus_tag="LAF92_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBI9793613.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(5057..6043)
                     /locus_tag="LAF92_004722"
     CDS             complement(5057..6043)
                     /locus_tag="LAF92_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBI9793614.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         1888 a         1428 c         1463 g         1518 t
ORIGIN      
        1 acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca gcaaatgctg
       61 gcctgatttt ttcttgaggg aagactatga tgcgcaaaat gctgctggcg gcagcacttt
      121 cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg cgtgacgatg
      181 tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg gtgatcacgc
      241 cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc cagcgcgagc
      301 aggcgaagga ttatcaggct gaactacgca gcacgctgcc gtggattgat gaaggcgcga
      361 aaagccgcgt cgaaaaagcc cgtattgcgc tggataaaat tatcgttcag gagatgggcg
      421 aaagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagctgaaa gagcagatga
      481 accgcattat cgaaacgcgc agcgatggcc tgacgtttca ctataaagcc attgatcagg
      541 ttcgcgccga aggccagcaa ttagtgaatc aggcaatggg tggaatttta caggacagca
      601 ttaatgaaat gggcgcgaaa gcggtgttga aaagcggcgg taacccatta cagaatgtgc
      661 tgggaagcct gggggggctg caatcctcaa tccaaaccga gtggaaaaag caggaaaaag
      721 atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa gatagccgca
      781 aagccctggt cgggaattta aaataatcct ctattttaag acggcataat acttttttat
      841 gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca aaatatattc
      901 acgttgtaaa ttgtttaacg tcaaatttcc catacagagc taagggataa tgcgtagcgt
      961 tcacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc cgcactggtt
     1021 atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc aaccggcggg
     1081 accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg taaagttggc
     1141 gtagaaaatc tggttaatgc ggtgccgcaa ctaaaagaca ttgcgaacgt taaaggcgag
     1201 caggtagtga atatcggctc ccaggacatg aacgataatg tctggctgac actggcgaaa
     1261 aaaattaaca ccgactgcga taaaaccgac ggcttcgtca ttacccacgg taccgacacg
     1321 atggaagaaa ccgcttactt cctcgacctg acggtgaaat gcgacaaacc ggtggtgatg
     1381 gtcggcgcaa tgcgcccgtc cacgtccatg agcgcagacg gtccattcaa cctgtataac
     1441 gcggtagtga ccgcagctga taaagcctcc gccaaccgtg gcgtgctggt agtgatgaat
     1501 gacaccgtgc ttgatggccg tgacgtcacc aaaaccaaca ccaccgacgt agcgaccttc
     1561 aagtctgtta actacggtcc tctgggttac attcacaacg gtaagattga ctaccagcgt
     1621 accccggcac gtaagcatac cagcgacacg ccattcgatg tctctaagct gaatgaactg
     1681 ccgaaagtcg gcattgttta taactacgct aacgcatccg atcttccggc taaagcactg
     1741 gtagatgcgg gctatgatgg catcgttagc gctggtgtgg gtaacggcaa cctgtataaa
     1801 tctgtgttcg acacgctggc gaccgccgcg aaaaacggta ctgcagtcgt gcgttcttcc
     1861 cgcgtaccga cgggcgctac cactcaggat gccgaagtgg atgatgcgaa atacggcttc
     1921 gtcgcctctg gcacgctgaa cccgcaaaaa gcgcgcgttc tgctgcaact ggctctgacg
     1981 caaaccaaag atccgcagca gatccagcag atcttcaatc agtactaatc gcctcgcccc
     2041 ggtatcgtgc cggggctttt tcacttcaga ctcacgtcca ttgccaattt taattaccct
     2101 aatgataatc accggaataa attattccgc gcgagggttt tcgggtgaaa aagcaatgga
     2161 ttgttggcac ggcactgctt atgttgatga ctggtaatgc ctgggcagat ggcgaaccgc
     2221 caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt ctcaagcttg
     2281 atgctatcac cttaaaaaat cttgatgcta agggtaatca ggccacctgg tcagcggaag
     2341 gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca gattacgaac
     2401 tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg ttaaccagta
     2461 aaggaacgcc cgcgtctggc tggtcggtga acttttactc ttttcaggcg gcagccagcg
     2521 atcgtgggcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc gtgaatagcg
     2581 aagatttcaa ttatcgcttt agtcagcttg aatctgcgtt gaatacccag aagaactcga
     2641 ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg gcgcagaaag
     2701 cagcggatgc atattgggga aaagatgcaa acggtaagca aatgacccgc gaagaagcct
     2761 ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc gaggcgttcg
     2821 cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat aaacagagtg
     2881 aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac gaacaacggc
     2941 gacagacttt tctgcaatca caaaaactca gccgtaaatt gcaggatgac tgggtaacgc
     3001 tcgaaaaagg gcaatatccg ttaacgatga aagtttcaga aataaacagt aaaaaagtcg
     3061 cgattttgat gaaaattgac gatatcaacc aagctaatga acgctggaaa aaagataccg
     3121 aacaactgcg gcgcaatggg gtgattaagt aagtttgata tagtgccttt gccggataat
     3181 ataaaaagtt atccggcaaa ttatttttta cattagccct agtagctgtg gaatgaacaa
     3241 ggatatttgc ggaatgtaag tgatcatcat caacgctact agcaatgcag cataaaatgg
     3301 cagcaatggt ttgattaaat gttgaatctt cacacccgag atagaacaac caacaaatag
     3361 cgcactaccc accggcggtg ttaacagccc aatacaaagg ttggccacca tcataatacc
     3421 gaaatgtact gggtccattc ccagctcttg tgcaatcggc aagaagatcg gtgtaaagat
     3481 tagtacagcc ggtgtcatgt ccataaagat gccgacaatt aataagacga tattaatcag
     3541 cagcaatatg atcagtgggt tatcagaaat ccccatcaat gcgtcgctga tcatataagg
     3601 aatatcagca ttagtcattg cccaggacat gcccacggaa aaaccgatca acagtaatac
     3661 aatcgaagtg gtaacaaccg actccagaat cagtttgggt aaatggcgcc attttacttc
     3721 ccggtaaacc agcaccgaga gaataaatgt gtagacaacg gcaattgctg acgcttcagt
     3781 tgcggtaaaa atgccgccca gaatgccgcc catcacaatg aacaccagaa gcaaacttgg
     3841 taaggcatcc agaaacgctt tacaggcctg agcaaaggtt gctctttctg aaagcgggta
     3901 gccacggcgt ttagcaataa tgccgcagac tatcattacc gcaagaccca ttaagatccc
     3961 tggcaaatat cccgccataa atagcgatgc tacagaaacg ccgccagctg tcagggaaaa
     4021 cacaattaac acgtttgagg gcggtatcag taatccagta atacaagatg agacgtttac
     4081 agcggtagaa aatgccggat cataaccttg tttggtctga atgggattca gtgtgccacc
     4141 aacggcagca gctgccgcaa cggctgaacc agaaatggaa ccaaacatca tattcgccag
     4201 gacgttcaca tgaccaagtg aaccaggaac ccgaccaacc attacctggg cgaggttaat
     4261 caaacgtatc gcaataccgc cgctgttcat cagcgttccg gcaaagatga aaaaggggat
     4321 tgccaatagg gcaaagttat ccagtccatt agccagtcgc tgcgaaacag tgattgccgc
     4381 gatatcccag ggaaacatca aaagcattga agctacagtg gcaataccga ttgagaacga
     4441 aatgggcaca ccaataaaga ccaacacaaa aaaacagcca aacagtgtaa tcgcgatata
     4501 actatccatt aactggcctc ctgcgcatgt tgtttcagtt gttgaatgct ttgattaata
     4561 aaacacaagg cataaaacat catcaccaat ccacttaatg gcagaatgat atatacgtat
     4621 cccatcggta tttgcatcgc ggcagatacc tgtgatgttg ccagcgtttt atcaattaat
     4681 ttcaggccac cagtcacaat gaccgaaccg gcaaaaccta aaataagaac gttaatgact
     4741 atactaagaa gtaattgctt gcgcttattc agggcgagag caaataaatc gatagagaga
     4801 tgtctctgcg cgccgacggt atatgcagca cctaataacc ctacccagat catcaaaaat
     4861 cgtgccagct catcggttaa tgtacttggc tggttaagta cgtagcggct aaatacttgc
     4921 cagactacgc atacaaccag agcaagcatg acggcgacag aaaacgcggc aatgacgcgg
     4981 tctaccgcaa gtttcaggcg gttaaatatc attgtaagca tcctggaaaa tcagggccag
     5041 agactggccc ggaatattat tgagcagcgt tgtcgaactt ctctaacaaa gcagcttgtt
     5101 ttggatcttt tttgaagtcg tcaaacagag gctgaactgc ggcacggaat ggtgctttat
     5161 ccacttttac aatttcaccc cccatagctt tcgcctgagc acgtgtgtca gcgtcaattt
     5221 tttcccacaa tttttgttga taagcttcgg atttcttcgc tgctgtttcg aggatttgtt
     5281 gctgttctgg ggttaattta ttccatgttt tggtggagat aaccaaaaag tcaggaattg
     5341 aggcgtgttc atcttcagaa aaaactttgg caatttcaat atgtcgagtc tgcacccagg
     5401 aaggcacgtt attttctgcg ccatctacta caccttgctg catcgcagta taaacttcgc
     5461 caaatgaaat tggagtgggt gaaccaccca tcagttcaat cattttgatg gtggtcggac
     5521 ttggctgaac acgaattttc agccctttaa gatcttctgg cttagtaatc ggttttttcg
     5581 catagaaact gcgtgtaccc gctacataag ctgataaagc aaaaaagcct ttgtcttttg
     5641 ttgagtccat aatttcttta ccaacttcac caaaaacaac tttgttgaag tgagcctgat
     5701 ctttaaataa gaacggcaaa ttatagatag cataaatatt atcaaaagac tccagatcac
     5761 tggcggaacc tttggtcata tccagagcgc cattttgcaa caactccata gtttctcgcg
     5821 cattacccat ctggctgctg ggataaatac ggatcaccat attacctttt gacagctgtt
     5881 ttacctcttt cgccagctct tcaaaactct gatgtacaac atgacttctt tccaggttgt
     5941 gtgctaactt taacgtcact ttttctgcgg cgtttacgcc agtgctaacc agagccagca
     6001 aggatgcgca gactaatgag cgggtaaatg acctcatttt cataaaaaaa ctccctgttg
     6061 aaggatggaa attggttgat atctaattgt gcttatcagt gctttgtgcg acaaattggt
     6121 taacctgtct gacaacttgt ctatgtttat cacaacttga aataaagtca gaaattaaat
     6181 attaaatttg tgcgccaggt tgtacatgtt tataatcaat aaatgacgag taaatattca
     6241 ctatatatat ataataaata tcgtcgatat ttattcagta agaaatatat tttgcca
//
LOCUS       DAEVZA010000202         6279 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEVZA010000202 DAEVZA010000000
VERSION     DAEVZA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.202"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            305..1300
                     /locus_tag="LAF92_004723"
     CDS             305..1300
                     /locus_tag="LAF92_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBI9793615.1"
                     /translation="MATTVIAAFNEFMKDTVNIKKADTDDARASRDWLIGKMNDFEKD
                     DKFPVSFPAIHIAFGSFARRTKIRPLDDIDLMFGLTGQGATYTILSDRITVTSSGEGS
                     RLHSYRHSGADTVCSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIV
                     PCFITSEDAFGRTYYLIPDGNGHWKFTDPRKDRDRVTTINVQNNGNVLNVIRAVKYWQ
                     RRPTMPSMSSYLLETLILDYYAGRTSCSSFVDMELEALFRHLGQSVRYSVNDPKGIQG
                     DINSLSAEARKAISDRCYLDAQKVSEARWFENNKEYEKSINKWRDVFGPFFPVYG"
     gene            1304..2236
                     /locus_tag="LAF92_004724"
     CDS             1304..2236
                     /locus_tag="LAF92_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793616.1"
                     /translation="MNAIYTNQSTRENLDLLYAQARVYDRVKNWNRLNFLFSIIVPLL
                     LSFVTVYNRSREFVDSELLSSLLGLYGLLVLTFNIVISGHISALRRKAASIQEMYDCR
                     VLGIRRNELKVEEIPRDDIIREAAYFRDSPEKARKRFGEEGWYVSKVYDAPQAVMALL
                     CHGKNLGWDKSLREVLHVFYLSAFIVSPVAMLVYGIVMKSGLNEILFYMVFTLPVIRY
                     FLLQFLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLGYTLRNIQDEMFTYRASC
                     PPVPNGIQLIMKPKNERIYVDYFETNLKELHLQK"
     gene            2534..3622
                     /locus_tag="LAF92_004725"
     CDS             2534..3622
                     /locus_tag="LAF92_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI9793617.1"
                     /translation="MLSQLTLRFHKKLIEALKIRAGHENTSVNALAARFLDDGLKTAA
                     AGDGYFQLVADPEATVRQLYRYIILGQTFGTAPVSRDELRFILAYAREAFICGQNRLA
                     TLPALRTLLDITRDLLAWQAENDRTVDRHYLQGIFRLPGDNLVEEFDRFLADLRPVVD
                     QMYAEHLLRPLESGCFELTEIPDAVLAEIFTLPRLNTIFPLVLRGLDWTTDKATALAQ
                     DLRPVIPTVTETVEAGTLRLEIRIDGQHPGERPGAWYNTPRLHLLITGQDFVVPYGWE
                     VFSELLGLFTLYARHPEALAHGHQGEHVMLSPPGHVSKEGFFGIDGLRIFMPAEAFET
                     LVRELTIGCAQGSLAEALTGLRGLYGDV"
     gene            3624..4493
                     /locus_tag="LAF92_004726"
     CDS             3624..4493
                     /locus_tag="LAF92_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793618.1"
                     /translation="MVSFTLLENALDSVEIGLEYFRKSRESQNRRDYKQCLLNLFQAA
                     ELLLKAAVSRQDAGRIFTQKSLKKHCKDPACPTESELHRCQSVKISELCELIKTHHRS
                     EFSDHGLQLMRAVAQMRNNLQHFALEISPQSLAMQLSELYKLIFRPAFILLQADKEHN
                     SWNSAARRQFIDLEAHFMEITTPDEYVLALCPVCESWSHFIVYDGESYPVSSHCICCN
                     FRLNNLQTWDFQECPECNWHSVIYVPQYHAGVCLCHTCYYSKEGGFVPMEPCKCSGFR
                     MEDHCSNCDPEEE"
     gene            complement(4550..6115)
                     /locus_tag="LAF92_004727"
     CDS             complement(4550..6115)
                     /locus_tag="LAF92_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9793619.1"
                     /translation="MAARQNIPAQLRLKEIQIQSLKGISNCTITFPADRKVTAIMGMN
                     GSGKSTIIHALACCYKPRNKTSKENNRFSDFFTPHDDNNWRDSGFTAHFYAGNLDNQG
                     NKISFVPTAAPNDTFTQVYAKVSRWQPVYARRPVKESLYLGLQTLGTLSDDLAASRHA
                     KYVSQSFEPANIKQKILESMCRVLEADYSDLMTCTTSSGYTFSKFTKNGITYTEHTMG
                     AGEKRVFEVLKAAHDPLLRPNGLLLIDELDVLLHEKAFKKLIEELISIAEDALLEIVF
                     STHRESIVQFKRAINIVSIFNMGTGIQAFPGVSADALRQLTDIQPEMVSVFVEDELAR
                     TVINVLLEREALTDKVNVELFGAAENSAVVLAGLMLAGEDINKVMCVLDGDVHRTVSE
                     RHKIVNKCLTGTDKREQRRAVMNRIFEFSLADFPQKGAPEYNHKRWFEAIDPATISAE
                     ESAEYIKLRGFSRSINGLADWHDYYEKLNHFARKSNIEHTVLSYISKYSPAWDDYISA
                     IRQEIIVKAAELE"
BASE COUNT         1636 a         1498 c         1510 g         1635 t
ORIGIN      
        1 acttttttcg gatctctgga gaagggaaga aggatttgtg ctgataatgc aatactaatg
       61 atatattttg aacttaattg cattattgct tatttgtcat taataactga aatacattgc
      121 ataattatca atgaaagaac gaggctcata tttcccatgt aaattatatg cccttgtaga
      181 gagtgagacg gtagtgaact tgcggtacta agaataaact gtaccgactc ttcctgggca
      241 ctggaatgtt cagatgtacg acagagccct gcgccttttg taataaataa ttaaaggact
      301 gaacatggcg accacagtaa tagctgcctt taacgaattt atgaaagata ccgtgaatat
      361 caaaaaggca gataccgatg acgcgcgtgc aagtcgcgac tggcttatcg gtaagatgaa
      421 tgattttgag aaggacgata aatttccggt gagttttcca gcgatccata ttgcctttgg
      481 ctcctttgcc agaagaacca aaatccgtcc gcttgatgat atcgatctga tgtttggctt
      541 aaccgggcaa ggtgccacct ataccatcct gagtgaccgg ataactgtaa cctcctccgg
      601 agaggggtca cgtctgcaca gttaccgcca ttccggagca gacaccgtct gctccgtcag
      661 aattctgaat gcctttaaaa accgcctgca ggacattgcc cagtacgccc aagctgatat
      721 caggcgtaac caggaagctg tcacgctgaa actggtcagc aaggactgga attttgacat
      781 cgtcccctgc tttattacca gcgaagatgc ctttggacgg acttactacc tgatccccga
      841 tggcaacggc cactggaagt ttaccgaccc ccgtaaagac agggacagag tcactactat
      901 caatgtgcag aataacggca atgtgctcaa tgtcatcaga gcagtcaaat actggcagcg
      961 gcgaccgacg atgccatcca tgagttccta cctgctggaa acgctgattc tggactatta
     1021 cgctggcagg acatcctgct catcgtttgt tgacatggag ctggaagcgc ttttccgcca
     1081 ccttggtcaa tccgtgcggt actctgtcaa cgatcccaaa ggcatacagg gtgatatcaa
     1141 ctcactttca gccgaggcac gtaaggcgat atcggacaga tgttatctcg atgcgcaaaa
     1201 ggtatcggag gcgagatggt ttgaaaataa taaagagtat gaaaaatcca tcaataaatg
     1261 gcgcgatgtg ttcgggccgt tctttcctgt ttacgggtga accatgaatg ccatttacac
     1321 aaaccagtcg accagagaaa acctggattt actgtatgcg caggcccgtg tctacgacag
     1381 ggtaaaaaac tggaacagac tgaattttct cttcagcatc atcgtaccgc tcctgctttc
     1441 tttcgttacc gtatacaacc ggagccggga atttgtcgat tcagagctgt tgtcttcgct
     1501 gttggggtta tatggactcc tggtgcttac ctttaacatt gttatcagtg gacacatctc
     1561 ggctctgcgc aggaaggctg cctcaatcca ggaaatgtac gactgccggg tacttggcat
     1621 ccggcgtaat gaactgaagg tggaggagat cccacgggat gacatcatcc gggaagctgc
     1681 atattttaga gacagcccgg aaaaagcacg aaagcgattt ggcgaagagg ggtggtacgt
     1741 cagtaaggtg tatgacgcgc cgcaggccgt aatggcgctc ctttgccacg gaaaaaatct
     1801 cggctgggac aaatcgctca gggaggttct gcacgtattt tatctttccg cttttattgt
     1861 ctcgcctgtc gcaatgcttg tttacggcat cgtcatgaaa tccgggctga atgagatcct
     1921 cttctatatg gtgttcacac tgccggttat ccgctatttc ttgcttcagt ttctggataa
     1981 tcgcagcagt atgaaaagaa gcgagaagct gaaaaagtac gttgagaagg aattatccgg
     2041 cataagggtg tccggaaggg cagaagaaga gcaactgggt tatacgctcc ggaatatcca
     2101 agatgagatg tttacctacc gagcctcatg tccgcccgtt cccaacggta ttcagctgat
     2161 aatgaagccc aaaaatgaac gaatttatgt tgattacttt gaaacaaatc tgaaggagct
     2221 gcaccttcag aagtaatccc tccttagctc cggtgtagga tttacgtatg ccggagcaca
     2281 tccgataaac aattctggtt gcctgagtgt cagcgtaaac acatagtcac cttcacctca
     2341 ggacggttag catccagctt tcacagaaac cggctctcag tgaagaaaga agaataacgc
     2401 tgaatcacat tattgaaaat gacatcatgt catttaattg actgcattgt tatcaaatga
     2461 tttacttcat acatattcat ttcatttatt gaaactgtat tcaaagaata tagctgtcat
     2521 gacaggaacc cacatgctga gtcagctgac cctgcgcttt cacaaaaaac tgatcgaggc
     2581 actgaaaatt cgtgccggcc acgaaaatac ctcagtcaac gccctcgccg cacgctttct
     2641 tgatgatggt ctgaaaacgg ccgctgccgg cgacggttat tttcagcttg tcgccgatcc
     2701 ggaggccacc gtccggcaac tgtaccgata tatcattctc ggccagacct tcggcaccgc
     2761 tccggtctcc cgggatgagc tacgcttcat cctagcctat gcccgggagg cctttatctg
     2821 cggccagaac aggctggcaa cactcccggc cctgcgcacc ctgctcgaca tcacccgcga
     2881 cctgctggcc tggcaggctg agaacgaccg cacggtggac aggcactatc tgcaggggat
     2941 attccgcctg ccgggagata acctggtgga agaatttgac cgattcctag cagatctgcg
     3001 tcctgttgtg gatcagatgt atgccgagca cctgctgcgc ccgctggaga gcggctgctt
     3061 tgaacttacg gagattccag acgcggtcct ggcggaaatt tttaccctgc cgcggctgaa
     3121 taccatattt cccctggtcc tgcgcgggct ggactggact acagacaaag ccacagccct
     3181 cgcacaggat ctgcgcccgg tgatcccgac cgtgacggaa accgtcgaag ccggcacgct
     3241 tcgcctcgag atccgcattg acggccagca tcccggcgag cgtccggggg cctggtacaa
     3301 tacaccccgt ctgcacctgc tcatcaccgg ccaggatttt gtggtgccgt acggctggga
     3361 agtattctct gaactgctgg gcctgttcac gctttatgcc cgtcatcctg aagctctggc
     3421 acacggccat cagggcgagc atgtaatgct ctcacccccc ggacacgtat caaaagaggg
     3481 attcttcggt atcgacggac tacggatttt tatgccggcg gaggcctttg agacgctggt
     3541 ccgtgagctc accataggat gtgctcaagg ctcgctggcg gaggccctga ctgggctacg
     3601 cggcctttac ggagatgtct gacatggtga gttttacctt actggaaaat gcgctggact
     3661 ctgtagagat aggactagaa tatttcagaa aatcccggga aagccagaac cgacgtgatt
     3721 acaaacaatg cctgcttaat ctcttccaag cagcagagtt gttgctgaag gcagccgttt
     3781 cgcgtcagga tgctggaagg attttcacgc aaaaatcctt gaaaaagcat tgtaaggatc
     3841 ctgcatgtcc cacagaaagt gagctgcacc gatgccagtc tgtgaagatt agcgaattat
     3901 gtgaacttat aaaaactcat caccggagtg aattttcgga tcatgggtta cagttgatga
     3961 gagctgtagc acagatgaga aataatcttc agcattttgc gctggaaatc agcccacaaa
     4021 gcctggcgat gcagttgtct gaattgtaca aattgatctt tcggcctgcg ttcatcttgc
     4081 tacaggccga taaagagcat aattcctgga actcagccgc gcgcagacaa tttattgatc
     4141 tggaggctca ttttatggag attacaaccc cggatgaata tgtgcttgca ctttgtcccg
     4201 tctgcgaaag ctggtcgcac tttattgtct atgacggtga aagttatccg gtcagctctc
     4261 attgtatctg ttgtaatttc cgtctgaata acctgcaaac atgggatttt caggagtgtc
     4321 cagagtgtaa ctggcattca gtaatctatg tacctcagta ccacgcggga gtatgccttt
     4381 gtcatacctg ttactacagc aaggaaggag gatttgttcc aatggagcca tgcaaatgca
     4441 gtggattccg gatggaagat cattgcagta actgcgatcc agaggaagag taaaccgttc
     4501 ggcagataag tgagtcagca gggcagcaaa agtaccgccc tgaatattct cattccaatt
     4561 cggcagcctt gacaataatt tcttggcgga tcgctgaaat ataatcatcc cacgcgggag
     4621 agtatttgct gatgtagctc agaacggtat gttcgatgtt ggattttctg gcaaaatgat
     4681 tcagtttttc atagtaatca tgccagtctg ccagcccgtt gattgaccta gaaaaccctc
     4741 ttagcttgat gtattcagca gactcctctg ccgaaatagt ggcagggtct atcgcttcaa
     4801 accagcgctt atgattatac tccggggcac ctttttgagg gaaatcagca agactgaact
     4861 caaaaatgcg gttcataacc gcacgacgtt gttctctttt atctgtccct gtcaggcatt
     4921 tgtttactat tttatgtctc tcagagacag ttcggtgaac atcgccgtca agaacacaca
     4981 taaccttatt aatatcctcg ccggcaagca ttagtccagc cagaacaacc gccgagtttt
     5041 cagctgcacc gaacagttca acattcacct tatcggtcag agcttctcgc tccagaagca
     5101 cattgataac cgtgcgtgcc agttcatctt caacgaaaac actgaccatt tcaggctgaa
     5161 tatcggtcag ttgtcgcaac gcatcagctg acaccccagg gaaagcttga atccctgttc
     5221 ccatattgaa aatactgaca atattgatgg ctcgcttgaa ctgaacaata gattccctgt
     5281 gcgttgagaa gacaatttca agcaaggcgt cttcggctat ggaaatcaat tcttcaatca
     5341 gctttttaaa agccttttca tgcaaaagta catccagttc gtcaatcagt agtaaaccat
     5401 taggtctcag aagggggtca tgagcggctt tcagcacttc aaaaacccgt ttctccccag
     5461 ctcccattgt atgttcagtg taggtaattc cgtttttggt aaatttactg aatgtgtagc
     5521 cactgctggt cgtacaggtc ataagatctg agtaatccgc ttcaagcacg cgacacatag
     5581 attcaagtat tttttgtttg atattagcgg gttcgaagct ttggcttaca tatttcgcat
     5641 gacgtgaagc agccagatca tcggacaacg tgcctagcgt ttgcagaccc agatagagcg
     5701 actccttgac tggacgtcgt gcatatactg gctgccagcg actgactttg gcatacactt
     5761 gggtaaaggt atcattcggt gctgctgtgg ggacaaatga aattttgttg ccctgattgt
     5821 ccaggtttcc tgcgtaaaaa tgggctgtaa agccactgtc acgccagttg ttatcatcgt
     5881 gaggagtaaa gaaatctgaa aatctgttgt tttcctttga ggttttattc cggggtttgt
     5941 aacaacaggc aagggcatga ataatcgtgg atttaccgga gccgttcatc cccataatgg
     6001 cagtaacttt gcggtcagca ggaaatgtga ttgtacagtt tgaaatgcct ttgaggctct
     6061 gaatctgtat ttccttcaag cgtaattgtg ctggaatgtt ttgcctagct gccattctga
     6121 gcggttcctt tgcatgctaa tagtcggatt aatcccaagc acattacctt aattcaggga
     6181 aaaagtctgg catctaaaac agagatttca caggcagata ttaccttttc cgacttaacc
     6241 ttttttcgga tctccgataa aaaagaaaga aatagggat
//
LOCUS       DAEVZA010000203         6259 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEVZA010000203 DAEVZA010000000
VERSION     DAEVZA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.203"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..371
                     /locus_tag="LAF92_004728"
     CDS             <1..371
                     /locus_tag="LAF92_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793620.1"
                     /translation="QTINKATSIYKIEHITVGKPGSEQYRHAFELADQLGLKHPDCIE
                     HVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHENGGIGYQF
                     LYEGDELKCYEHGLLIESVE"
     gene            698..1558
                     /gene="repA"
                     /locus_tag="LAF92_004729"
     CDS             698..1558
                     /gene="repA"
                     /locus_tag="LAF92_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HBI9793621.1"
                     /translation="MNQSFISDILYADIESKAKELTVNSNNTVQPVALMRLGVFVPKP
                     SKSKGESKEIDATKAFSQLEIAKAEGYDDIKITGPRLDMDTDFKTWIGVIYAFSKYGL
                     SSNTIQLSFQEFAKACGFPSKRLDAKLRLTIHESLGRLRNKGIAFKRGKDAKGGYQTG
                     LLKVGRFDADLDLIELEADSKLWELFQLDYRVLLQHHALRALPKKEAAQAIYTFIESL
                     PQNPLPLSFARIRERLALQSAVGEQNRIIKKAIEQLKTIGYLDCSIEKKGRESFVIVH
                     SRNPKLKLPE"
     gene            1958..3163
                     /locus_tag="LAF92_004730"
     CDS             1958..3163
                     /locus_tag="LAF92_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9793622.1"
                     /translation="MKRDYGGVGTIALRASALLKAMSQDIEDQRKEFNQTEYYQTFTR
                     NAVAKLPKLSRRIVEQAIKEMEDDGYQFNKKQVGNVEQYALTIQNVIDIYAHRKIPKY
                     RDIHKSPYVIFVVNLKGGVSKTVSTVTLAHALRVHQDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGSILETAAQAMLNDLDAETLRKEVIRPTIVPGVDVIPASIDDGFVASQWK
                     ELVEEHLPGQNQYEILRRNIIDRVADDYDFIFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLEQLEEEGVEPRLSASIGFMSKMTGKRDHETSHSLAREV
                     YASNILDSSLPRLDGFERCGESFDTVISANPQSYPGSAEALKKARTEAERFTKAVFDR
                     IEFVRGEAA"
     gene            3160..4125
                     /locus_tag="LAF92_004731"
     CDS             3160..4125
                     /locus_tag="LAF92_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBI9793623.1"
                     /translation="MKKIVSRGRVLGKNSSEFARMLEGSEGTKTFTLKSGRQAKFLLT
                     VVLSGEIESRTFVDPAVNGRDQSLLTPESVSDISRTIKLQQFFPAIGRMVGERIEVLD
                     GSRRRAACIFNETKFEILVTKDEISLADARQLAIDIQTAREHTLRELGKRFEVMYGKS
                     MTKEEIARAENISKAKVTRAFQAAAVPDEMIAVFPVASDLALPDYQLLLQISEDANAK
                     SVPIEELVDTVRERIAETEGAKEDKAKILAIFKAESKSLKPAPVKSVVVEKLRDFSDR
                     RQYARKKSDPKKRVVAYEFSRLPSEVQTEIDEAIKKIIGKMSAGE"
     gene            4390..6096
                     /locus_tag="LAF92_004732"
     CDS             4390..6096
                     /locus_tag="LAF92_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793624.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYVAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFDV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPVLVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISSDTIDAGD
                     SE"
BASE COUNT         1618 a         1547 c         1524 g         1570 t
ORIGIN      
        1 agcaaactat caacaaagca acttcaatct acaaaattga gcacatcact gttggcaagc
       61 caggatctga acaataccgt catgctttcg agcttgccga tcagcttggt ttaaaacacc
      121 cggattgcat cgagcatgta tttccgacct atgctgatga gcaatgtact catgtcctta
      181 ccgaagagga ttttttcagc actgaagaac gagaaggcgt tgatcgctgc attggtgtga
      241 tttgctcttc agtgagttat gagttattcc ctaatgtcca tgaaaatggt ggtattggat
      301 accaattcct gtacgaaggc gatgagctta aatgttatga acatggtctt cttatcgaaa
      361 gcgtagaata atacccttcc ttccaaccgg ctatgttggc cggtttttca cttatccaca
      421 ttatccacat tatccactgg gtagatccaa taattaggtc catacagatc ccaattagat
      481 ccatatagat ccctgatcgt tgcaggccgc gccacgtctg gcttagaagt gtatcgcgat
      541 gtgtgctgga gggaaaacga tgtgtgctgg agggataaaa atgtgtgctg acgggttgct
      601 aatgtgtgct ggcgggatat aggatgtgtg ctgacgggaa agcctgggta gttatcacca
      661 cttataaaaa ctatccacat aattcggaaa aagtaatatg aatcaatcat ttatctccga
      721 tattctttac gcagacattg aaagtaaggc aaaagaacta acagttaatt caaacaacac
      781 tgtgcagcct gtagcgttga tgcgcttggg ggtattcgtt ccgaagccat caaagagcaa
      841 aggagaaagt aaagagattg atgccaccaa agcgttttcc cagctggaga tagctaaagc
      901 cgagggttac gatgatatta aaatcaccgg tcctcgactc gatatggata ctgatttcaa
      961 aacgtggatc ggtgtcatct acgcgttcag caaatacggc ttgtcttcaa acaccatcca
     1021 gttatcgttt caggagttcg ctaaagcctg tggtttcccc tcaaaacgtc tggatgcgaa
     1081 actgcgttta accattcatg aatcacttgg acgcttgcgt aacaagggta tcgcttttaa
     1141 gcgcggaaaa gatgctaaag gcggctatca gactggtctg ctgaaggtcg ggcgttttga
     1201 tgctgacctt gatctgatag agctggaggc cgattcgaag ctctgggagc tgttccagct
     1261 tgattatcgc gttctgttgc aacaccacgc cttgcgtgcc cttccgaaga aagaagctgc
     1321 acaagccatt tacactttca tcgaaagcct tccgcagaac ccgttgccgc tatcgttcgc
     1381 gcgaatccgt gagcgcctgg ctttgcagtc agctgttggc gagcaaaacc gtatcattaa
     1441 gaaagcgata gaacagctta aaacaatcgg ctatctcgac tgttcaattg agaagaaagg
     1501 ccgggaaagt tttgtaatcg tccattctcg caatccaaag ctgaaacttc ccgaataagt
     1561 gtgtgctgga gggcagctgc atttgaaaaa tgtgcgctgc cgggaatgcc tgcccatttt
     1621 cctgtttttg gtgtgcgctg gagggttgcg ccacgcagtt tgcccagaca ttccctccag
     1681 cacacatcta accatccaag tttccctcca gcgcacatct aatcttctat ctttcccttc
     1741 agcgcacata tttgatacca gcgatccctc cacaacacat aattcaatgc gacttccctc
     1801 tatcgcacat tctggtcctg catcatccct ccagcacaca tctaatagcc tcatcgccat
     1861 ttctttacgt gcaataattt acgcacgaat caaaataagt tgcacgtagc agaatcaaac
     1921 gtacaattca ctcatacgaa atgataagga gatgatgatg aaacgcgatt acggcggtgt
     1981 cggcaccata gctcttcgtg caagcgcatt acttaaggcc atgagtcagg atattgaaga
     2041 tcagcgcaaa gagttcaatc agaccgagta ttatcagacg ttcactcgta acgctgtggc
     2101 aaagttgccg aagctgagcc gccgcattgt ggagcaggcc atcaaagaga tggaagatga
     2161 tgggtaccag ttcaacaaga aacaggtcgg taacgttgaa cagtacgcgc tgaccatcca
     2221 gaacgtcatt gatatctatg cccaccgtaa gatcccaaaa tatcgcgaca ttcacaaatc
     2281 gccttacgtt atttttgtcg taaacctgaa gggtggcgta tccaaaacgg tttccacagt
     2341 cacgttggcg cacgctctgc gtgtgcatca ggatttactg cgtcacgatc tgcgcattct
     2401 ggtaattgac cttgaccctc aggcatccag cacaatgttc ctcgaccata ctcacagtat
     2461 tggttccatc ctggaaaccg ccgcgcaggc gatgctgaac gacttggacg cggagacgct
     2521 acgcaaagag gtgattcgtc cgaccatcgt tcctggcgta gacgtgattc cagcctctat
     2581 cgacgatggc tttgttgcca gccaatggaa agagctggtt gaagagcatc ttcccggaca
     2641 aaatcagtac gaaatccttc gacgcaatat cattgatcgt gttgcggatg attatgactt
     2701 tatctttatt gataccggtc cacacctgga tccgttcctg ctcaacggtc tggcggccag
     2761 cgatttgctg cttaccccta ccccaccagc ccaggttgac ttccactcaa cactgaaata
     2821 tctcacccgt ctgccagaaa tgctggagca actggaggag gaaggcgtag aaccgcgttt
     2881 aagcgccagc attggtttta tgtcgaagat gaccggcaag cgcgatcacg agacatcaca
     2941 cagccttgcg cgtgaggttt acgccagcaa cattctggac tcttctctgc ctcgtctgga
     3001 tggatttgag cgatgcggcg agtctttcga caccgtaatc agtgccaacc cgcaatcgta
     3061 tccaggcagt gcagaggcgc tgaagaaggc acgaaccgag gccgagcgtt tcactaaggc
     3121 tgtgtttgat cgaattgagt ttgttagggg tgaggcggca tgaaaaaaat agtttcccgt
     3181 ggacgagtgc tgggcaagaa tagctccgag tttgctcgca tgcttgaagg cagtgaaggc
     3241 accaaaacct ttaccctaaa atctggccgc caggctaaat ttttgcttac cgttgtgctg
     3301 agtggtgaga ttgagtcgcg cacgttcgtt gacccggcag ttaacggccg cgatcagtct
     3361 ctgctcaccc ctgagtcggt aagcgatatt tcccgcacca ttaaattgca acagttcttc
     3421 ccggctatcg gtcgtatggt tggggagcgc attgaggtat tggacggatc acgtcgccgc
     3481 gctgcgtgta tcttcaatga aacgaaattt gagattctgg tgacgaaaga tgagatcagc
     3541 ctggcggatg cacgccagct ggccattgat atccagacag cccgcgaaca caccctgcgc
     3601 gaactgggta aacgcttcga ggttatgtat ggtaagagca tgaccaaaga agagatcgcc
     3661 cgcgctgaga acatttcaaa ggctaaagtt acgcgcgcgt tccaggcggc agcggtgccg
     3721 gatgagatga ttgctgtctt ccctgttgcc agcgatctcg ccctgccaga ttaccagtta
     3781 ctgctccaga tctccgagga tgccaacgct aaaagcgtgc ctattgaaga gctggttgat
     3841 acggtgcgcg aacgaattgc agagactgag ggcgcgaaag aggataaagc gaagatactg
     3901 gctatcttca aagcggaaag caaaagcctg aagcccgcgc cggttaaatc tgtggtggtt
     3961 gagaagctgc gagacttctc tgaccgtcgc caatatgctc gaaagaagtc cgatccgaaa
     4021 aaacgggttg tcgcctacga gttctccaga ctcccgtctg aagtgcaaac tgaaattgac
     4081 gaagcaataa aaaaaatcat tgggaaaatg tctgctgggg aataatcccg ctggtgggag
     4141 gtggtgttag cccctcccct tcctaaaatg tcccgcacca atttcattac taaccttatg
     4201 atttgcatgt gttttttagg agaatttcag actgaaattc ccacgattta acgcctgcat
     4261 tcaccgtcca cttccccgca caaaaaattt aaaaaattac ttttcgcgag aaagtcaaca
     4321 agtgactttc aataaaatct cttccgaaaa gggattcaca caagtgcctt gtgtttaagg
     4381 aagagtaaat tgagtaactt acgcgaatac cagaatcgta ttgcagatat cgcaaaacgc
     4441 tctaaagctg tgcttggctg ggcaagcact gcgcagttcg gtactgataa ccaattcatt
     4501 aaagatgatg ccgcgcgtgc cgcatctatc cttgaagctg cacgtaaaga cccggttttt
     4561 gcgggtatct ctgataatgc caccgctcaa atcgctacag cgtgggcaag tgcactggct
     4621 gactacgccg cagcacataa atctatgccg cgtccggaaa ttctggcctc ctgccaccag
     4681 acgctggaaa actgcctgat tgagtccacc cgcaatagca tggatgccac taataaagcg
     4741 atgctggaat ccgtcgcagc agagatgatg agcgtttctg acggtgttat gcgtctgcct
     4801 ttattcctcg cgatgatcct gcctgttcag ttgggggcag ctaccgctga tgcgtgtacc
     4861 ttcattccgg ttacgcgtga ccagtccgac atctatgaag tctttaacgt ggcaggttca
     4921 tcttttggtt cttatgctgc tggtgatgtt ctggacatgc aatccgtcgg tgtgtacagc
     4981 cagttacgtc gccgctatgt gctggtggca agctccgatg gcaccagcaa aaccgcaacc
     5041 ttcaagatgg aagacttcga aggccagaat gtaccaatcc gaaaaggtcg cactaacatc
     5101 tacgttaacc gtattaagtc tgttgttgat aacggttccg gcagcctact tcactcgttt
     5161 actaatgctg ctggtgagca aatcactgtt acctgctctc tgaactacaa cattggtcag
     5221 attgccctgt cgttctccaa agcgccggat aaaggcactg agatcgcaat tgagacggaa
     5281 atcaatattg aagccgctcc tgagctgatc ccgctgatca accacgaaat gaagaaatac
     5341 accctgttcc caagtcagtt cgttatcgcg gctgagcaca cggtacaggc ggcgtatgta
     5401 gcacagcgtg aatttggtct ggacctgggt tccctacagt tccgcaccct gaaggaatac
     5461 ctgtctcatg aacaggatat gctgcgtctt cgcatcatga tctggcgtac tcttgcgacc
     5521 gacacctttg acatcgctct gccggttaac cagtcctttg atgtatgggc aaccatcatt
     5581 cgtggcaaat tccagactgt atatcgcgac attattgagc gcgttaaatc ttctggtgcg
     5641 atggggatgt ttgctggtgc tgatgcagca tctttcttca aacagttgcc gaaggatttc
     5701 ttccagccag ccgaagacta tatccagact ccgtatgttc actacatcgg taccctgttc
     5761 ggtaacgtga aagtgtacga agtacctgct ggtatttgta agaacttaac gacagagaac
     5821 attcagttca gctcgatgga tgtgctgtgc tacgtccgtg atgaaaatcc gggtaaagca
     5881 ggcttcgtga ctggtgatgc tgtcccggcc atcccgttcc agcatccgac cactccggtg
     5941 ctggtcaacc gtaccacgct gtggggttcg gctatcaacg atatgcaccc acgcaacggc
     6001 gctgattact tcactcgtgt aacgctgaca atggccaaaa aaggcgggct taactttata
     6061 agcagcgaca cgattgatgc cggtgactct gagtaatcag gggaagttct ccgtttaaca
     6121 tagcgccccg tgcggggcgc ataacaggga aagttatgtc tcaatattca attcaacagt
     6181 cattaggtaa tgcatccggt gtcgctgtta gcccgatcaa tgccgatgcg acgttatcta
     6241 ccggtgttgc attaaatag
//
LOCUS       DAEVZA010000204         6252 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEVZA010000204 DAEVZA010000000
VERSION     DAEVZA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.204"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..117)
                     /locus_tag="LAF92_004733"
     CDS             complement(<1..117)
                     /locus_tag="LAF92_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebG family protein"
                     /protein_id="HBI9793625.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLD"
     gene            251..1429
                     /gene="purT"
                     /locus_tag="LAF92_004734"
     CDS             251..1429
                     /gene="purT"
                     /locus_tag="LAF92_004734"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBI9793626.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     SDAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(1485..2126)
                     /gene="kdgA"
                     /locus_tag="LAF92_004735"
                     /gene_synonym="eda"
     CDS             complement(1485..2126)
                     /gene="kdgA"
                     /locus_tag="LAF92_004735"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBI9793627.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(2163..3974)
                     /gene="edd"
                     /locus_tag="LAF92_004736"
     CDS             complement(2163..3974)
                     /gene="edd"
                     /locus_tag="LAF92_004736"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBI9793628.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(4209..5684)
                     /gene="zwf"
                     /locus_tag="LAF92_004737"
     CDS             complement(4209..5684)
                     /gene="zwf"
                     /locus_tag="LAF92_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBI9793629.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSREIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            6022..>6252
                     /gene="hexR"
                     /locus_tag="LAF92_004738"
                     /gene_synonym="yebK"
     CDS             6022..>6252
                     /gene="hexR"
                     /locus_tag="LAF92_004738"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBI9793630.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANG"
BASE COUNT         1493 a         1666 c         1546 g         1547 t
ORIGIN      
        1 gtcaagcaga tccgccgtat caagcatttt gtcataagca tcggcttcct ttttgctggt
       61 aaacgacatt ttctcttcgc cctcacgaat gactacgtat ttaacttcaa ccgccatttg
      121 cagcctctca taataactgt gattttatac agtatatttc ttttcggttg agaaatcaac
      181 atcagcaata aagacacacg caaacgtttt cgtttatact gcgcgcggaa ttaatcaggg
      241 gatattcgtt atgacgttat taggcactgc gctgcgtccg gcagcaactc gcgtgatgtt
      301 attaggctcc ggtgaactgg gtaaagaagt ggcaatcgag tgtcagcgtc tcggcgtaga
      361 ggtgattgcc gtcgatcgct attccgacgc accagccatg catgtcgcgc atcgctccca
      421 tgtcattaat atgcttgatg gtgatgcatt acgccgtgtg gttgaactgg aaaaaccaca
      481 ttatatcgtg ccggagatcg aagctattgc caccgatatg ctgatccaac ttgaagagga
      541 aggactgaat gttgtcccct gcgctcgcgc aacgaaatta acgatgaatc gcgagggtat
      601 ccgtcgcctg gcggcagaag agctgcagct gcccacttcc acttatcgtt ttgccgatag
      661 cgaaagcctt ttccgcgagg cggttgccgc cattggctat ccctgcattg taaaaccggt
      721 gatgagctct tccggcaagg ggcagacgtt tattcgctct gcagagcaac ttgctcaggc
      781 atgggaatac gctcagcaag gcggtcgcgc cggagcgggc cgcgtaattg ttgaaggcgt
      841 cgttaagttt gacttcgaaa ttaccctgct aaccgtcagc gcggtggatg gcgtccattt
      901 ctgtgcacca gtaggtcatc gccaggaaga tggcgactac cgtgaatcct ggcaaccaca
      961 gcaaatgagc ccgcttgccc ttgaacgtgc gcaggagatt gcccgtaaag tggtgctggc
     1021 actgggcggt tatggtctgt ttggtgtcga gctgtttgtc tgtggtgatg aggtgatttt
     1081 cagtgaggtc tcccctcgtc cacatgatac cgggatggtg acgttaattt ctcaagatct
     1141 ctcagagttt gccctgcatg tacgtgcctt cctcggactt cctgttggcg ggatccgtca
     1201 gtatggtcct gcagcttctg ccgttattct gccacaactg accagtcaga atgtcacgtt
     1261 tgataatgtg cagaatgccg taggcgcaga tttgcagatt cgtttatttg gtaagccgga
     1321 aattgatggc agccgtcgtc tgggtgttgc gctggcgacc gctgagagtg ttgttgacgc
     1381 cattgaacgc gcgaagcacg ccgccggaca ggtaaaagta cagggttaaa cccggcaaaa
     1441 aaacgctaca aaaatgcccg atcgtagatc gggcgttttg acttttacag cttagcgcct
     1501 tctacagctt cacgcgccag cttagtaatg cggtcgtaat cgcccgcttc cagcgcatct
     1561 gccggaacca gccaggaacc accgatgcac agcacgcttt tcagcgccag gtagtcacgg
     1621 tagttagccg gagaaatacc acccgtcggg cagaaacgga cctgggagaa cggacccgcg
     1681 atcgcctgca gggctttcac gccgccgtta gcttcagccg ggaagaattt gaactctttc
     1741 aaaccgtagt ccatacccag catcagttcg gaaacagtgc tgatccccgg aattagagga
     1801 atagtccctt cggtagcagc tttcagcagc ggctcggtca gacccgggct aattgcgaac
     1861 tgtgcacccg cttcagtgac ttctgccagc tgctgtggat tcagcaccgt accggcaccc
     1921 acaatcgctt caggcacttc tttggcgata gcacggatag cgtcaactgc acactcggta
     1981 cgcagagtca cttccagaac gcgcacccca ccagcaacca acgcttttgc catcggcact
     2041 gcgtgttcca gtttttttac cacgataacc ggtacaaccg ggccggtggt caggattgat
     2101 tctgcacttg ttttccagtt tttcatcaga gttttctctc gcctgattac aaatttgtcg
     2161 tcttaaaaag tgatacaggt tgcgccctgt tcggcaccgg acagtttttc acgcaaggcg
     2221 ctgaataatt cacgtcctgt ccccacgcgt gacgcgctca ggtcaggaat gtgcggttcg
     2281 cgagcagcca gttccgcttc gtctaccagc agcgtcagtt cgcctgtctg tccattcaca
     2341 cgaatgatgt ccccgtcgcg cacttttgcc agcagcccgc catcgtaggc ttctggtgtt
     2401 acgtggatag ctgacggcac tttacctgaa gcgccggaga gtcgtccatc ggtaactaac
     2461 gcaattttga aacaccggtc caataataca ccaagtggcg gcatgagttt atgtaattct
     2521 ggcattccgt tcgcttttgg cccctgatga cggacaacaa cgacacaatc gcggtccagc
     2581 aaacccgctt caaaggccgg cataacgtca tgctggcttt caaaaacaac cgctggcgct
     2641 tcaatcacct ggttctcaac cggcacggca gaggttttca taaccgcacg gcccaggtta
     2701 ccgcttaaca cttttgtccc accatgatga gagaaaggtt gttcgaagga agcgatcaca
     2761 ttgctgtcga gtgatttttc cgccccttcc cgccagtcca gttcaccatt attcagccat
     2821 ggttcaaggg tataacgaga cagaccaaag cctgccaccg tattgacatc ttcatgcagc
     2881 aggcctgctt tgagcagttc acgcaccaga accggtacgc cacctgccgc ctggaagtgg
     2941 ttaatatcgg ccggaccgtt cgggtagaga cgtgccatca gcggtacaac atcagaaagg
     3001 tcagagaagt catcccagtt aatctgaata ccggccgcgc gcgccatcgc caccaggtgc
     3061 atggtgtggt tagtggaacc accggtcgcc agcagtgcaa cgataccgtt caccaccact
     3121 ttctcatcga tcatcttacc gatcggcatc cattcattac cattaccggt catgcgtgta
     3181 acctgacgcg cagcggcagc ggtcaaagca tcgcgcagcg gagaatccgg atgaacaaaa
     3241 gaagagcctg gcaactgcat ccccataaat tccaccacca tctggttggt gttggcagta
     3301 ccgtagaaag tacatgttcc cggcgcatgg taagacgcgg cttctgactc cagtaaggcc
     3361 atgcggtcca ctttaccttc ggcataaagc tggcgaatac gcactttttc tttatttggc
     3421 aaaccgcttg ccatcggtcc agacggcaca aacaccgcag gcaaatgacc aaacgacagg
     3481 gctgccatcg tcagacccgg gacaatcttg tcgcacacac cgaggaacag agcaccatca
     3541 aacatgttat gggacagccc gaccgccgca gacatcgcta tcacttcgcg gcttagcagc
     3601 gacaattcca ttccatcctg cccctgggtg acaccatcac acatcgccgg aacaccgccc
     3661 gcaacctgac caaccgcatt cgcttcatgc agggctttac gaatgatttc tggatagtgt
     3721 tcataaggct ggtgcgcgga gagcatgtcg ttataggagg tgatgatggc gatattgtta
     3781 cgcaacatgc ttttcaaaga ggctttgtct tctggctggc aggcagcgaa accgtgtgcc
     3841 aggttaccgc atgccaactg cgaacgatga acggtcgaag ttttcgcttg ttctatccgg
     3901 gcgagataag cagagcgagt ctcgcgcgaa cgttcaatga ttcgatttgt tacgcgtaac
     3961 aattgtggat tcataaaggc tcctgaaatt gagttgtcag agcaggatga ttcacaacgc
     4021 gtttcattca gaggatttat gactgaaacg cctgtaaccg gagctcatag ggcaaaaacg
     4081 tttcagtcag tgtaataaaa aaagcctcgt gggtgaatcc gcacgaggcc tgaaagtgta
     4141 aaaattgttc tacaatctgt gcaagatcat gttaccggta aaataaccat aaaggataag
     4201 cgcagatatt actcaaactc attccaggaa cgaccatcac gggtaatcat cgccaccgag
     4261 gcaacgggtc cccaggttcc ggcctgatac ggtttcggcg catcattgtc catcgcccac
     4321 gcctcagtaa tggagtctac ccatttccag gcttcttcca cttcgtcgcg acgaacaaac
     4381 agtgcctgaa taccacgcat ggtttccagc agcaaacgtt cataggcatc cgccagatgc
     4441 gtctgattaa aggtttctga atagctcaga tccagcttgg tgatttgcag gttatgtttg
     4501 tggtcaaggc caggaacttt attcagtacc tggatatcca cgccttcatc aggttgcaga
     4561 cggatagtca gtttattctg cggcagatcc tgccacgatt ctttaaacag attcagttca
     4621 ggtgttttga aatagaccac gacttcagaa catttggtcg gcagacgttt accagtacga
     4681 aggtagaatg gcacaccggc ccagcgccag ttatcaatgt cgacgcggat cgctacgaag
     4741 gtttctgtat tgctgctctt gttcgcgccc tcttcttcca gatatcccgg cacttttttg
     4801 ccctgggcga agcccgcagt atattgcccg cgtacggttt tttcgcgtac gttggagcgg
     4861 tcgatgcggc gcagagactt cagtactttc actttttcat cgcggatgct gtctgcgctc
     4921 aggtcagacg gcggagacat cgcaatcatg cagagaattt gcagcaggtg gttttggatc
     4981 atatcgcgca tctgaccggc tttatcaaaa tagccccagc gcccttcgat ccccacttct
     5041 tctgccacgg taatctcaac atgatcaatg gtgcgattgt cccagttatt cacaaacaga
     5101 gagttagcaa aacgcagcgc caacaggttc agcactgttt ctttaccaag atagtggtcg
     5161 atacggtaaa cctggcactc ctcgaagtat tcgccaacct gatcgttgat ttcccgcgag
     5221 gtcgccagcg acgtccccag cggtttctcc atgactacgc gtgccggttt agcattcagt
     5281 tttgcctcac caagcccttt gcaaattgcg ccaaaagtgc tgggcggcat ggcaaagtag
     5341 ttaatggtga tacgattttt ttgatccagc atcgcgccga gacggctgaa tgcagcagtg
     5401 tcattgacat cgagattaca aaaatccaga cgtgcactta gggtgtccca taaaccttca
     5461 tcaatggttt ctttcatgaa agtttcgagc gcctcgcgga caactttggt ataagccgct
     5521 ttatcccagt cagcacgccc tacgccgata atccgggtgt ccgggttgag ctgaccggct
     5581 ttttccagtt gatacaggga aggcagcaat ttacgacgcg caaggtcgcc tttcgcgccg
     5641 aaaatgacca ggtcacaggc ctgggctgtt tgcgttaccg ccatgtcatt ctccttaagt
     5701 taactaaccc ggtacttaag ccagggtata cttgtaattt tcttacggtg cactgtactg
     5761 cttttacgag cttgcgaaaa ctgtaaacgc ttatccaccc gtgcgattac ggtaaaagcg
     5821 cgcaaagtgc ggcaaaactg ataaaaaaat catcgttttc cattgggtaa aaatctgaca
     5881 ctgatcatgt tatgaaaaaa aataacaact tttttatctg cttttgtcat taacacagca
     5941 cacaggcgta atatctgaca aaactacatt tcgatttctt tcagtgcgga aatcgtcatt
     6001 acccgtgagt ttctttacat catgaatatg ctggaaaaaa tccagtctca gctggaacat
     6061 ttgagcaaat cagagcgcaa agttgccgag gtcattctgg cttcgcccga taacgcgatc
     6121 cattcgagta ttgctgctct ggcgctggaa gccaatgtta gcgaaccgac ggtgaatcgt
     6181 ttctgtcgca gcatggacac gcgcggtttt cctgatttta aacttcatct ggcacaaagt
     6241 ctggcgaatg gc
//
LOCUS       DAEVZA010000205         6225 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEVZA010000205 DAEVZA010000000
VERSION     DAEVZA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.205"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..600)
                     /gene="hcr"
                     /locus_tag="LAF92_004739"
     CDS             complement(<1..600)
                     /gene="hcr"
                     /locus_tag="LAF92_004739"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBI9793631.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMT"
     gene            complement(612..2264)
                     /gene="hcp"
                     /locus_tag="LAF92_004740"
                     /gene_synonym="priS"
     CDS             complement(612..2264)
                     /gene="hcp"
                     /locus_tag="LAF92_004740"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBI9793632.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(2408..3307)
                     /gene="lysO"
                     /locus_tag="LAF92_004741"
     CDS             complement(2408..3307)
                     /gene="lysO"
                     /locus_tag="LAF92_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBI9793633.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(3802..4497)
                     /gene="aqpZ"
                     /locus_tag="LAF92_004742"
     CDS             complement(3802..4497)
                     /gene="aqpZ"
                     /locus_tag="LAF92_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBI9793634.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            4923..>6225
                     /locus_tag="LAF92_004743"
     CDS             4923..>6225
                     /locus_tag="LAF92_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBI9793635.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWH"
BASE COUNT         1611 a         1600 c         1615 g         1399 t
ORIGIN      
        1 ggtcatcacg gtacgtgaag ctaagtcagg tacacctgcc agcagttcgc gagtgagacg
       61 accagcgata aagccttcgg taacgttatt ttctgccacc agcgttaccg gatagttacg
      121 ccactcatcg gcgaaaataa catcctgcgg cgtacgcacg ttgtagatca cccgcacatc
      181 ggcctgtgga cggttcttcg caagccagcg acgcatcgac ataatcggcg tgacgccgca
      241 gcctgccgcc agcaacagga atttatcttc tgctttatcg tcgcaggtaa attcccccat
      301 cgcgtccgaa agccagagat aatcaccgcg ttttacatcg cgcgtcagcc actgggagcc
      361 gacaccgtca tcaatccgcc gcacggtcag ggtgatatat tcactcacgc ctggcgtgga
      421 ggaaatggtg taagcacgca gcgtttccgc tgagtttcgc acgctgacca gtgcatattg
      481 cccggcgcga tatgggtagt aatcgtggca aatcagggaa atcgtccaca catccggcgt
      541 ttcttgcgta atgtgatgaa cctgcatccg ccacgggcat tgattcgttg gcatcgtcat
      601 cgacaaactc cttacgcgct caacagttgc ttcatgtctt cttcaacagt ggtgatagaa
      661 cgcaggccaa atttctcgtt cagcaccgcc agcaggtctg gtgtcaggaa accaggtgca
      721 gtcgggccgg tgacgatatt tttcacgccc agagaaagca gcgtcagcag aatgacgatc
      781 gctttctgtt caaaccagga gagcaccagc gacagcggca gatcgttgac accgcagccc
      841 agtttctctg ccagagtgac agccagaata atcgctgagt aagcatcgtt acattgacct
      901 gcatctacca gacgcggcag accttcgata tcgccaaact caagtttgtt aaagcgatat
      961 ttaccacagg cgagggtcag gatcaggcag tcatccggca cgctggtggc gaaatcggtg
     1021 aagtagtggc gctcgccgcg tgcgccgtca cagccaccaa gcaggaagat atgacgcagt
     1081 ttttcacggc tcaccagatc aatcagcgta tccgctgcgc caagcagcgt ctggcggcca
     1141 aagcccacgg tgatcaggtg cggaatttcg ctgtacggga agcccgccat ttgttgcgcc
     1201 tgggcgatga ccgcagaaaa atcttcgcca tccagatgac gcacacccgg ccagccaacg
     1261 atgctgcggg tccagatacg atcgtcataa gcgcctacgg ttgggtcgat gatgcagttc
     1321 gaggtcatca cgatggggcc agggaaacga gcgaactcca cttgctgatt ctgccagccg
     1381 ctgccgtagt taccgaccag atgcttgaat ttacgcagct ccgggtagcc atgcgcaggc
     1441 agcatttcgc cgtgggtgta aacattaacg cccgtgcctt cggtctgttc cagcaggttg
     1501 tagagatctt tgagatcgtg accggaaatc aggatgcatt tacccgccgt cgctttgacg
     1561 ttgacctggg ttggcgtcgg gtgaccgtat ttaccggttt cgcctgcatc cagaatgctc
     1621 atcactttga agttcatctg gccgatttcc attgaacact caagaagcgc gttcatatcg
     1681 gcaggccagg tccccagcca cgccatgatt ttatgatact gggcataaat atcgttgtcg
     1741 tattgaccga gaacatgcgc gtgttccata taggccgctg cacctttcag gccatacagg
     1801 cacagcagac gcaggccgag aatgttttcg ccaatcgccg ctttatcttt gttaggggta
     1861 aattctgctg cctgacgttg cagctcgccg agatcgtcgc tcaccagttg caggtcagcc
     1921 atcgggttat cgacgcgcgc gttggcatct acagccaggc attgcgcttt cagcgcctcg
     1981 cgcagggcaa tcgcttcacg agcgtagccg acaatacgcg gagaatcgaa gttaacgttg
     2041 gtcagggttg agaaaaaggc acgtggcgcg aagctgtcaa catcgtggtt gatgatgccg
     2101 tattcacgcg cttttaccgc ccaggcagaa agcccttgca gcgccgcgat gagtaaatcc
     2161 tgaaggtcag aagtttccgc cgttttacca cacatcccct gcgcgtatga gcagccgttt
     2221 cctgccggag tacggatagt ttgttcacat tgcacacaaa acatgatcac accttttaaa
     2281 gttatattta atatacatgt ttaaggttaa gacgcttaac gcggggataa aagggatttt
     2341 tcatgcaact ttaagggaga ttgatttagc gcaattttgg cggcagggat ctaccgccag
     2401 agaggtatta cgcagagaaa aaggcgatga ggatcggcac taacaggcta agaataaaac
     2461 cgtgaacaat tgccgccggg accatatcca gcccgccagt acgttgaaga acgggcaggg
     2521 tgaaatccat tgatgtggca ccgcataagc ccagtgcagt agagcggctg cggcgaatca
     2581 gcccagggat caacataata gcaatcagtt cacgggccag atcattaaaa aacgccgcgc
     2641 tcccgattac cggaccaaaa gattcggtca ataaaatacc ggaaagagaa taccagccga
     2701 aaccggaggc cattgccagc gcggtattga tggggagatc aagaataaag gcgttaatta
     2761 aaccaccaat taatgaactg acaaccacca ccacggcgac aatcattccc cggcgattaa
     2821 ggacaatctg ctttaaggtc atgccattat tgcgcaactg aataccaacg aggaaaagta
     2881 gcaaaattaa cgtgtattca ctggcttcgg tcgcgtgttg taagaaagcc agtccactca
     2941 gaccaatggc aaaaccaatc actactacgc cgcacagttt tagcgactcc agcgccatcg
     3001 caatacgcga cgggagtttt tcttgctgat ggtggttgcg ccacggcagg cctcgctcca
     3061 gccacatcag ggcggcaata ttacacagta aaataacggt aatactgacg gcagaataat
     3121 gcagaatcgc caacaggtta ctggcgaggt tatcgagaaa cgccagactg atacccataa
     3181 aaaagagaat aaggtaaacc atccagctta atagctgatt aataactttt aacgcagctt
     3241 gttggcgaag cggaatgagg taacccacaa tcaggggaac cagaatgatt aacagcccag
     3301 aaaacatgaa aacccagtcc ttgcaaagat gaagtcgaaa tgcgcgatga cacactactg
     3361 aaagcggaag gacgagtaaa gttgcaatta aaaggaaatg ttatgcataa ggagcagtag
     3421 agtattcgtt ttcatttaaa gatattcttg cgctttaatt acaaactgca ccgatgttgg
     3481 tggcgtcaaa atcgccgagg cgttccctga aggccggggc agcccacatg gatgtgggct
     3541 gagggcgcgt tttacaggga tgttacctcg cgcccgaccc ggtagccgta agggataagt
     3601 cgagggcacc gcgcagcggc gattttgttc gccagagccc gggggtgcag ggggcggcgg
     3661 cgattggccg ccccctgcgc gctccttgcg ccagtggcaa tatgtggctt agctcatgaa
     3721 aggagcgcaa caagatgatg aatcaacata taacaacatc ttaaaaaaag gcctgacatt
     3781 acgccaggcc ttctgcgtta attaatcacg cttttccagc agggtccggt aaatcagacc
     3841 accgataatg ccgccgacaa ttggcaccac ccagaagaac cacagttgtt ctaatgccca
     3901 gccgccctgg aagatagcaa ccgcggtgct gcgcgccggg ttaacagaag tgttagtcac
     3961 cggaatacta attaagtgaa tcagggttaa ggccagacca atagcgatcg gcgcaaaacc
     4021 tgccggcgcg aatttgtcgg ttgcgccgtg gatcaccaac aggaaacctg cactcaatac
     4081 cagttcaact accagcgcgg aaagcatgga ataaccgcct ggtgaatgct cgccataacc
     4141 gttagaagca aaaccgctgg ctgccgcgtc aaaacccgtt ttaccactgg caattaaata
     4201 cagcagcgcc gctgcaacaa taccgccgac aacctgggca attacgtagc caacgacttc
     4261 ttttgccgga aaacgtccgc cagcccataa accaatagtg accgccgggt taaaatgacc
     4321 accagaaata tgaccaacag caaaggccat cgtcagaacg gtcagaccga acgccaacgc
     4381 cacgccggca aaaccaatgc ctaattccgg gaagcctgcg gccagtacag cactaccaca
     4441 gccaccaaaa acaagccaga aagtaccaaa acattcagct gctaattttc tgaacatatc
     4501 cacctcaatt aaaaattgac cctgtgaaaa atatggtcgt tttatagggt cgtcgtaaaa
     4561 aagtgacgac ggaaataatg cgcggctatt ttaaaaacga aggcgagtca ttcaccagat
     4621 aaataaatcc agtaaatttg atttagggca acagcgggtt gccccatata gtcatttgtc
     4681 tgattgacag tgtagtgcaa gcaaaagatt taatccttta ggcgtaataa aaaataattt
     4741 atcatgctaa ttatttgatt ttgttgtttt tgcagactta tcagcaagag ggagtataac
     4801 gcgattgttc gctcattttt cagacatttg ccatgcttaa atgtgatgtc atcacgtatt
     4861 agcaaggcct ttcccgttat actgccagcg taaaggataa gtcacatatt tctggagggg
     4921 atatgattct tgagcgcgtt gaaattgtgg gttttcgcgg tatcaaccgt ttgtcgttga
     4981 tgctcgaaca aaacaacgtc ctgattgggg agaacgcgtg gggtaaatcc agcttgctgg
     5041 acgccttaac cctgctgcta tcgccagaat cagatctcta ccattttgag cgcgacgatt
     5101 tctggttccc gccgggagat atcaacgggc gagaacatca tctgcatatt attttgacct
     5161 tccgcgaatc gctgccaggc cgacatcggg ttcgccgtta tcggccgctg gaagcgtgct
     5221 ggacgccatg caccgatggc tatcaccgta ttttttatcg tctggaaggg gagagtgcgg
     5281 aagacggcag cgtgatgaca ctgcgcagtt ttctcgataa agacggacat ccgattgatg
     5341 tcgaggatat taacgatcag gcacgccatc tggtgcgttt aatgccggtg ctgcgcttgc
     5401 gtgatgcccg ttttatgcgc cgtattcgta acggcacggt gccaaatgtt cccaatgtgg
     5461 aagtcaccgc gcgccagctc gatttcctcg cccgtgagtt gtcctcacat ccgcaaaatc
     5521 tctctgatgg acagattcgt cagggacttt ccgcaatggt gcagttgctt gagcattatt
     5581 tctctgagca gggggccgga caggcgcgat atcgtttaat gcggcggcgg gccagcaatg
     5641 agcaacgaag ctggcgctat ctggacatca tcaaccggat gattgaccga cctggagggc
     5701 gctcgtatcg ggttattttg ctcggcctgt ttgctacttt attgcaggca aaaggcacat
     5761 tgagactgga taaagacgcg cgtccattgt tgctgatcga agatccagaa acccgtttac
     5821 accccattat gctttcagtt gcctggcatc tgttgaatct tctgccattg cagcgcattg
     5881 ccaccaccaa ctcgggtgag ttgctttcgt taacgccggt agagcatgtt tgccgactgg
     5941 tacgtgagtc ctcgcgcgtt gccgcctggc gtctggggcc gagtggcttg agtaccgaag
     6001 atagccgacg catatccttt cacattcgtt ttaaccgtcc gtcatcgctg tttgctcgct
     6061 gctggttgct ggtggaaggg gaaacggaaa cctgggttat caatgaactg gcgcgtcagt
     6121 gcggacatca ttttgatgcc gaagggatca aggtcattga gtttgcccag tccgggctaa
     6181 agccactggt taaatttgcc cgccgaatgg ggattgaatg gcatg
//
LOCUS       DAEVZA010000206         6059 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEVZA010000206 DAEVZA010000000
VERSION     DAEVZA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.206"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..753
                     /locus_tag="LAF92_004744"
     CDS             <1..753
                     /locus_tag="LAF92_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HBI9793636.1"
                     /translation="KSNKNDFVDAEAICEAASRPSMRFVQPRTESQQAMRALHRVRES
                     LVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLSTLLEDSSLPLYLSQLLLKLQQHYH
                     YLVEQIKDLESQLKRKLDEDEVGQRLLSIPCVGTLTASTISTEIGDGKQYASSRDFAA
                     ATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVFIQKLEHQSGKLADWVRELL
                     CRKSNFVVTCALANKLARIAWALTARQQTYEA"
     gene            complement(935..1111)
                     /locus_tag="LAF92_004745"
     CDS             complement(935..1111)
                     /locus_tag="LAF92_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBI9793637.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            1441..2256
                     /gene="sul2"
                     /locus_tag="LAF92_004746"
     CDS             1441..2256
                     /gene="sul2"
                     /locus_tag="LAF92_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBI9793638.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2343..2645
                     /locus_tag="LAF92_004747"
     CDS             2343..2645
                     /locus_tag="LAF92_004747"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793639.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2821..4314)
                     /locus_tag="LAF92_004748"
     CDS             complement(2821..4314)
                     /locus_tag="LAF92_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HBI9793640.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4525..4749)
                     /locus_tag="LAF92_004749"
     CDS             complement(4525..4749)
                     /locus_tag="LAF92_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793641.1"
                     /translation="MKKPNQDDEPFFITEEIAAEMIAGGYEFELPPIPCTIRLRDVLE
                     RMTDAELALQPGEIADQERERCRRKPCSTS"
     gene            complement(4746..5483)
                     /locus_tag="LAF92_004750"
     CDS             complement(4746..5483)
                     /locus_tag="LAF92_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI9793642.1"
                     /translation="MRRTKPVAAPMVARVYLRVSTDAQDLERQEAITTAAKAAGYYVA
                     GIYREKASGARADRPELLRMIGDLQPGEVVIAEKIDRISRLPLPEAERLVASIQAKGA
                     SLAVPGVVDLSDLAAEAQGVAKIVLEAVQIMLFRLALQMARDDYEDRRERQRQGIELA
                     RQAGRYKGRRADPKRRAQVVALRKSGYSINKTAELAGYSAAQVKRIWAEVSQAEAKQH
                     GAFVEDALTEADALAAVGQDERQEERA"
     gene            5810..>6059
                     /locus_tag="LAF92_004751"
     CDS             5810..>6059
                     /locus_tag="LAF92_004751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793643.1"
                     /translation="MRRCSSEAKKNRLGVSEAASLHAVQGGRNRAGDQGGKPRGLMCS
                     SATLAGFFDLSDASGPPWALLDLLLLFLDVLLLLVLLLV"
BASE COUNT         1205 a         1781 c         1732 g         1341 t
ORIGIN      
        1 aaaagcaaca aaaatgactt cgttgatgct gaagctatct gtgaagcagc atcacgtcca
       61 tctatgcgtt tcgtgcagcc cagaaccgaa tctcagcagg caatgcgagc tctgcatcgt
      121 gtccgtgaat ccctggttca ggataaggtg aaaacaacta atcagatgca tgcttttctg
      181 ctggaatttg gtatcagcgt tccgcgaggt gctgccgtta ttagtcgact gagtaccctt
      241 cttgaggaca gtagtttgcc tctttatctc agccagttac tgctgaaatt acaacagcat
      301 tatcactatc ttgttgagca gattaaagat ctggaatctc agttgaaacg aaagttggac
      361 gaagatgagg ttggacagcg cttgctgagt attccctgcg ttggaacgct gactgccagt
      421 actatttcaa ctgagattgg cgacgggaag cagtacgcca gcagccgtga ctttgcggcg
      481 gcaacagggc tggtaccccg acagtacagc acgggaggtc ggacgacatt gttagggatt
      541 agcaagcggg gcaacaaaaa gatccgaact ttgttggttc agtgtgccag ggtattcata
      601 caaaaactgg aacaccagtc tggcaagttg gccgactggg tcagggagtt gttgtgtcgg
      661 aaaagcaact ttgtcgtcac ctgtgctctg gcaaacaagc tggccagaat agcctgggca
      721 ctgacggcgc gacagcaaac ttacgaagca taaaggcaga aatacaccag tttaaacaat
      781 cattcatctg gttttgcgaa tactgatatt gatgatacta acggcccacc ggcctgttga
      841 ggaacctgta aaacggaaag gctcattgaa gccgtatatt ttctggaggt tcatcaggcg
      901 cggaactcat cgaggcgcgg gaataaaatc ccattcagac gccggataga ttcaagcaag
      961 ccaacttgtc gtcaaaatcg gtgttgcaaa aacgggagtg accatagatt ccgttttctg
     1021 aggcgacccc cacatagaga ccttcccggt cattcacttt gtagagtttg tccctgggct
     1081 tgagattgcg cagcttggta tcggtcagca tgtaccttgt ccttcttcgt ctccgatacc
     1141 cactctattg taaacaagac atttttatct tttatattca atggcttatt ttcctgctaa
     1201 ttggtaatac catgaaaaat accatgctca gaaaaggctt aacaatattt tgaaaaattg
     1261 cctactgagc gctgccgcac agctccatag gccgctttcc tggctttgct tccagatgta
     1321 tgctattctg ctcctgcagc taatggatca ccgcaaacag gttactcgcc tggggattcc
     1381 ctttcgaccc gagcatccgt atgagactca tgctcgatta ttattattat agaagccccc
     1441 atgaataaat cgctcatcat tttcggcatc gtcaacataa cctcggacag tttctccgat
     1501 ggaggccggt atctggcgcc agacgcagcc attgcgcagg cgcgtaagct gatggccgag
     1561 ggggcagatg tgatcgacct cggtccggca tccagcaatc ccgacgccgc gcctgtttcg
     1621 tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg cgctcaaggc agatggcatt
     1681 cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg
     1741 gcctatctca atgatattcg cggttttcca gacgctgcgt tctatccgca attggcgaaa
     1801 tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag
     1861 gcacccgctg gcgacatcat ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg
     1921 ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg atcccggcat ggggtttttt
     1981 ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc ggttcgatga attgcggctg
     2041 cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca
     2101 ggccgtggtc cgggggatgt cggggccgcg acactcgctg cagagcttgc cgccgccgca
     2161 ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg gctggcggta
     2221 ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc acattcggga tatttctcta
     2281 tattcgcggt tcagcaggca tgtccccttt gagggcgacc cgacgacagg ataatcgacc
     2341 ttatggtgcg caaatatttc ggcacagacg gtattcgtgg caaagccaac gaaggcgcga
     2401 tgacggcgga aaccgccttg cgcgtcggca tggcggctgg ccgtgtcttt cgtcgcggtg
     2461 accaccgcca tcgtgtcgtg atcggcaagg atacgcgcct gtcgggctat atgcttgaac
     2521 ccgcgctcac agccggtttc acctcgatgg gcatggacgt attccttttt ggcccgctgc
     2581 cgacaacgta taggaagaat aaacgccctt ttcacccaag tccaacagct ttggaccgca
     2641 gttgactctt tcgacacccc tgcgatgcaa cccaatccgg ctgacgggga gccagcaacg
     2701 ctgaaaattt accctcctct ttcccactag cggctccttt tccgacaacc agcacggcgg
     2761 atccctgccg cggcgctgtg aacgcagcat tttgattggt atcgttggcc ttcaggctcg
     2821 tcagtcaaac agacccagga gcagctcagc cggtggcgcc cggctttcgg gtaacgccct
     2881 ggtcccgctg gtttcggctt tgtgcttcag gtgatcaagg atctgcttga tcactatagg
     2941 gtcttcaatg caggcgatga ctttcatggc gccgccgcag ccgctgcagg tctcgatgtc
     3001 gatattgaaa acacgcttga gccgttgcgc ccatgtcatc gacgctcgcc gttgtgctgg
     3061 tgttgccggt tcatcagcca ccctgacctt gttgcccctg ccccgttttg ccggcgtgac
     3121 caacgcccgg tgccgactgt tgggtgcgaa caccccgtgg aagcgggtta ggttgactct
     3181 gggcttcggt accagggcgg ccagccttgc aatgaaatcc aatggttcga aaatgacgtg
     3241 cgtggtgccg tcccggtacg gcgtcttgag ctggtagcgc acgttgccgc ctcgtgttaa
     3301 cgacagccgc ttctcggata ccgccgggcg gctgatgtac cggcacagcc gttcgagctt
     3361 cttgcgttca tcggccctgg ccgccacgcc ggcgtgcagg ctggacccgg ctaccttgcc
     3421 aatcccgtca ccgaacggat caccactggt cggcagagtt tgcaaagtga acacctttcg
     3481 ccccgcctgt gaaccgacag cgatacggta agtgatcgag tgccccagca ggggtgtcat
     3541 cgggtcgtca tccaccgcat ccgaggccag atagctgttt tcgacatccc gttccagcag
     3601 gccttgccgt tccagatagc gacccacccg gtgggcgatg gtgtgcgtca gctgggtgag
     3661 ctctgggctg gtcggcgcct tgacccagcg gaaacgcgct gagccgtggg attgctcgac
     3721 atacacaccg tcgagaaaca gcatgtggaa gtgaacattc agattgagcg ccgatccaaa
     3781 acgctggatc agggtgaccg cgcccgtctt ggccacttgg tgggtatggc ccgctttctt
     3841 gaccaggtgc gtggcaatga cgcggtaaac gatgcccagc acccacccca tgatctcggg
     3901 ccggctggca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     3961 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     4021 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     4081 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     4141 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     4201 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     4261 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     4321 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     4381 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactcgat
     4441 tgtaaacaag cacacaaaac cctttagtga caacaatttg caagaactat caggctcagt
     4501 gccatgaaaa ataccatgac cagatcatga ggttgaacac ggcttgcgcc ggcagcgttc
     4561 acgctcctgg tcggcgatct cgcccggctg caatgctagc tcagcatcgg tcatgcgctc
     4621 cagcacgtcg cgtaggcgga tggtgcaagg aatgggcggc agctcgaact catagccgcc
     4681 ggcgatcatt tcggccgcaa tctcctcggt gatgaaaaac ggctcgtcgt cttggttcgg
     4741 cttcttcatg ccctttcctc ctggcgctca tcctggccga cagcggccag ggcatcggct
     4801 tccgtcaatg cgtcctccac gaacgcgccg tgctgcttcg cttcggcctg gctgacctcg
     4861 gcccatatcc gtttcacctg ggccgcactg tacccggcca gctcggcggt cttgttgatg
     4921 ctgtagccgg acttgcgcag cgcgacaact tgggcgcggc gcttcggatc agcacggcgg
     4981 cccttgtacc gcccggcctg gcgggccaac tcaatgcctt ggcgctggcg ttcgcgcctg
     5041 tcctcgtagt cgtcgcgggc catctgcaag gccaggcgaa aaagcatgat ctgcacggct
     5101 tccagcacga tcttggcgac gccctgggcc tcggccgcca ggtcggatag atcgaccacg
     5161 ccagggacgg ccaggcttgc gcctttggcc tgtatcgagg ccaccaggcg ctcggcctcg
     5221 ggcaaaggta ggcggctgat gcggtcgatc ttctcggcaa tgaccacctc gccgggctgt
     5281 aggtcgccga tcatgcgcag cagctcaggc cggtcggcgc gtgcgccgga tgccttctca
     5341 cggtagatgc cggcgacgta gtagccggcg gccttcgcgg ccgtagtgat cgcctcttgg
     5401 cgttccaagt cctgcgcgtc ggtgctgacg cgcagataga cccgcgccac catcggcgcg
     5461 gctactggct tcgtcctgcg catacttgcg ggctcctttg ggcatacgca aagtgtccgg
     5521 tcatgctacc cccggccaaa aaggccgtca tgcgatttat cttggccggg tcgttattgg
     5581 cgggtctaaa tggccgctcc gtggatctca tcgccagaaa tggcgatgcg agatccaggg
     5641 cagggcggcc gccatcgtca gacctggcga tggtcgacgc cggcgcgcat cgtcggatgt
     5701 ggcgatgcga gctcgaggtc ggtggccacc gccggcgacc tggtgccatc gtcggcaatg
     5761 gcgatgctgc agctcgacgt gcgcggcctg gtcgtctcgg catcgtcgga tgcggcgatg
     5821 ctcgagcgag gcgaaaaaaa accgcctcgg ggtgtccgag gcggcaagtc tgcatgcggt
     5881 tcagggagga agaaaccgcg ccggcgatca gggaggaaaa ccgcgcggcc tgatgtgcag
     5941 ctcggccact ttagccggat ttttcgactt atccgatgcg agcggcccgc cgtgggcgct
     6001 gctcgatctg ctgttgcttt ttctcgacgt gcttctgctc ctggtgctgc tgctcgtcg
//
LOCUS       DAEVZA010000207         6041 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEVZA010000207 DAEVZA010000000
VERSION     DAEVZA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.207"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..300)
                     /gene="yliI"
                     /locus_tag="LAF92_004752"
     CDS             complement(<1..300)
                     /gene="yliI"
                     /locus_tag="LAF92_004752"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HBI9793644.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRI"
     gene            complement(411..794)
                     /gene="bssR"
                     /locus_tag="LAF92_004753"
     CDS             complement(411..794)
                     /gene="bssR"
                     /locus_tag="LAF92_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBI9793645.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            1007..2332
                     /gene="rimO"
                     /locus_tag="LAF92_004754"
     CDS             1007..2332
                     /gene="rimO"
                     /locus_tag="LAF92_004754"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBI9793646.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(2379..3707)
                     /locus_tag="LAF92_004755"
     CDS             complement(2379..3707)
                     /locus_tag="LAF92_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBI9793647.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(3715..>6041)
                     /locus_tag="LAF92_004756"
     CDS             complement(3715..>6041)
                     /locus_tag="LAF92_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI9793648.1"
                     /translation="KIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQK
                     NVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLF
                     SRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFD
                     RPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEII
                     AKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASD
                     KLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGD
                     AKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIK
                     LCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTR
                     KIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFC
                     DDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGI
                     ISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEP
                     HFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTG
                     LSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVA
                     RELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1686 a         1460 c         1294 g         1601 t
ORIGIN      
        1 gatgcggcga gactgagcaa aatcaggcgc taaaaccacg tccagcaggc cgccctgccc
       61 gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat aatccttttc ctgcttgcca
      121 gtgacgcaac tcgccgccgc gcagagtgat taacataccg tgattatcgg gtaaaaaggc
      181 cagtgcccag ggatggtcga gtttgtcttg cagtacttcg acatttaccg ttgcaggagc
      241 cgcccagaga gcggaagaaa gacaaataag gggcacaagg aaaaaggatt gtcgatgcat
      301 aacgcctccg gattgactct ggcttaaagc gtagtcagtg gaggagataa caaattcatt
      361 tttacaaaaa cttaaacatg aagggggaga cgctttctcc cccttagttt tcaggccttc
      421 tcaagcatgg cgtgcttctg caggctctgg atactcagcg ttaagctcat cagacaattt
      481 tcaagcttat cggcgttgac ggtaataaca gtcgggcaat catggtgccc actcatcaaa
      541 catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa gagcactcca ctcttcctga
      601 tccagatgaa gattcaaccg cagcgattta tcgtgcagtt cgcgattcag tttaaaaaag
      661 ttatctcgta gatgattgct ttcgctgacg gacatgtatc cttttgcctt cctcagttgc
      721 acgtatgcgg cgaggtcaac gcgtgcgtcg atcaaccggt tcagcagatc gattcgctgt
      781 ctgtcaacga acatacttcg atcctcctct ttccagcctt gttgcaatta aatagtatgg
      841 ctattttttg agcaaaatgc tgtccaggat caattcttca cgccgaatta acaaaattta
      901 catttccttc gcctccgcca gcgaatgaga ttgcgataca aattttatcc agaactctgt
      961 agaatccctg ccctgacctt tcctgtaact gaatattttt taagctatga gcaaagtaac
     1021 tccccagccg aaaatcggct ttgtttccct tggctgtccg aaaaaccttg ttgattcaga
     1081 gcgtattctc accgaactcc gcactgaagg ttatgacgtg gtaccgagct atgacgatgc
     1141 ggacatggtg atcgtcaaca cctgcggctt tattgacagc gcggtacaag aatcactgga
     1201 agccattggt gaagcgttga atgaaaacgg caaggttatt gtgaccggtt gtctgggggc
     1261 aaaagaagat cagatccgcg aagtccaccc gaaagtgctg gaaatcaccg ggcctcatag
     1321 ctatgagcag gttctggagc acgttcatca ctacgtgcca aaaccgaaac acaacccatt
     1381 cctgagcctg gtgccagaac aaggtgtgaa actgacgccg cgtcattatg cctatctgaa
     1441 aatttctgaa ggctgtaatc accgctgcac cttctgcatt attccgtcta tgcgcggcga
     1501 cctggtgagc cgtccgattg gcgaagtgtt aagtgaagcg aaacgtctgg tagatgcggg
     1561 cgttaaagag attctggtga tctcgcagga tacttccgcc tatggcgttg atgttaaaca
     1621 tcgtactggc ttccacaacg gcgagccggt aaaaaccagc atggtcagcc tgtgcgaaca
     1681 gttatcgaaa ctggggatct ggacacgtct gcactacgtt tacccttatc cgcatgtgga
     1741 cgacgtcatc ccactgatgg cagaaggcaa aatcctgccg tatctggaca ttccgttgca
     1801 gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg ggttctgtag atcgccaact
     1861 ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg accctacgct caacctttat
     1921 tgtcggcttc cctggcgaga cagaagaaga tttccagatg ctactcgact tcctgaaaga
     1981 agcgcgtctg gatcgcgttg gctgctttaa atacagcccg gttgaaggtg cagacgccaa
     2041 tgccctgcct gaccaggttc cggaagaagt taaagaagaa cgctggaacc gtttcatgca
     2101 gctgcagcag cagatttccg ccgagcgcct gcaagagaaa gtgggccgtg aaattctggt
     2161 gattatcgac gaagtggacg aagaaggcgc gattggtcgc agcatggcag atgcaccgga
     2221 aatcgacggc gcggtttatc tcaatggtga aaccaacgtt aagccgggtg atatcctgcg
     2281 tgtgaaagtc gagcacgccg atgagtacga tttgtggggt agccgggttt aattaattca
     2341 ggccgggcaa aatcatttgt ccggcctgcg aataatgatt actgccagct aatctgagcc
     2401 ctgccgtttt tcttactctt atacagccgc tcatccgcca gcattttcaa gcgttcgacg
     2461 gtggtacatt catttgaatg cgctacaccg atactggtac ttaaattaat gcttcccacc
     2521 tggaaatgaa cgatttcttt tagaatcgat agcaactctt ccagcgttcc acccgtataa
     2581 atcaccgcaa attcgtcacc gccgaagcgg tagattttac ctttatctac cgggatctgt
     2641 gatttaagga ttttcaccac tttcatcaac acttcatcgc ccagcaaatg accgaaggtg
     2701 tcgttaatgt ttttgaattt atcgacgtca aacaggcaca aataaccgcc tttttccggc
     2761 agcgctttta aatcttcgtc aaaggatttt ctgcccattg ctttggtcag gtcgtccagg
     2821 aacgccgagt tatacatact gtagagtttt ttgacatcgg cagccgaaaa gaaaagcccg
     2881 ccatccagta cgcgttcaaa atggttatta atgatggctt ctttctccac aataccacga
     2941 ttaatcactc tgcgtaccag atagaggcaa ctggtacccg atatcaccgc caccaatgcg
     3001 gcaaagaaaa taatcaatgt acagacccgc aaataaacat cttttttata cgcaaatacg
     3061 tacaatccgg tcggcaaata ggcgaagtgc tccatcgctt ccgaaacggt gaagatggcg
     3121 tttttagcat tatattcatc accggtcacc ggtttaccgt tcttaaatag agagaacatc
     3181 aggttctcgc tgtccatcgc cagcgatttt gacagtaaag aaaaacgctc cagaccaagc
     3241 aaaaatatca gactacgatt gtgagttacc ggatagaggt aatagacgaa ggttttaccg
     3301 tcaaaagaga taaaccctgt atttttgaca tgtcgatact tcacaacctg cataatatct
     3361 gcacaataat ttttctcgaa ctcctttacc tgaatacatt cgtcactgga aatcagataa
     3421 ggaccgacat cgtacaaata agagggggaa accgagtcgg cgtaagctgt gcgcaaacca
     3481 accaacagct gttcctccgt gggatttagc gtgttgataa gatcaattgc cacgctacgc
     3541 cgatattcag caatatttgc caccgcaaaa gcagtatcaa taacggactg tttcaggcta
     3601 tacacccgtt cctttaccat ttgagtgtag atcccgcagg caactgctga aatcatgata
     3661 aaaatcagca gactgactgt aagtacgaac ttattgattc tggacatacg taaactactc
     3721 ttttactaat tttccacttt tatcccaggc ggagaatggc atgggtttct ggtacagata
     3781 cccctgagcc tgaacacatc ctaatttaat taattcgcgc gcaacgtctt cgctctctac
     3841 gccttccgca atcaccggga tccggttata gcgcgccaga tcgaaaataa tgcgcacaat
     3901 ttttcccgca gtaccgaaaa tatcgccggt aaattgcgag tccactttta tgctatctgg
     3961 attaatttca taaaaacgtt tcaggtttga caaaccggtg ccaaagtcat caatcgatat
     4021 ccgtacccct tgtttacgca gttgttcaat attgagataa aaatgttcat taattcgctc
     4081 gatcacggtt tcagtgatct caacacagag gctgttcgcc ttcaggccat tttctttcat
     4141 gatggtggta aaacgctcat gaaagtgcgg ttcgtttaac tgccagggcg aaacattaat
     4201 atgcagtaag aaatcatcct tcagcgacgc acgctgacta aaatgtctaa attcacccat
     4261 tgccgtttta ataatctgat aacccagctc attgataaac ccgatatctt cagcaatggt
     4321 aatgaacttc agcggtgaaa tgatcccccg ctccgcagac acccaacggc acaacgcctc
     4381 cgcgcccaga atggcgcggg tgttaatatc aacgatcggt tgatagacga ggaatagttc
     4441 gccaatttcc attgcatggc gaatattacg cgcaatccag atattctctt ctatttcgtt
     4501 gagtttcgcg tcatcacaaa aaataaactc ggagaacata tggtcacgga tactggaaat
     4561 cgccagtttc agtttttctg cgtaactcca ggcgttttcc ccctgcaaag gttcacaaat
     4621 ggcagactta tggatgcgca gattgcggta gccaaagctg ctgtcaaaat cgtttaccat
     4681 cgctatttta cgggtaaatg tctgtacatt ttcccgacac agaagtaaat agagatcggc
     4741 attgaatgtc acgatatcgc agcattgcgc atatttctcg gcaattcgct gacacaattt
     4801 cctgacgatt ttattgcgtt catcaacacc aaataccgct tccacggctt ccagaccaca
     4861 cagtttgatg atccccacag ccagatactg attattgttg gactcaataa tcgccatatt
     4921 tttaataaaa ccgctttcct gatcgtaaga gatcagtgag agcaatttat ttgaccgttc
     4981 aatttcggca tctttcattt ccgcgatcga atttttgatg ctgataatct ctttcgcatc
     5041 gccttcgcga attttcagag cctcgatttg atccgttgag tcgggcaaaa acgccagcgt
     5101 attaaccagc tcggtcattt cagaaaattg ccgacggaac acacgagtgg tcatgaaata
     5161 taccaccagc aacacgataa taatgaaagc aaacggtaga taaaaaagtt tatctgatgc
     5221 gttcaataaa ttaccgccag gataataaaa atagatttcg aacaatgcat tatttggcac
     5281 tgctgatgtc tttgcaatat actccgtatc gacagaatca ataatattat aattaccttc
     5341 gctattataa ataatcagtt tttcacgagc gagttgattc ttggcaatga tctcatgacc
     5401 ttgtttcaaa aatagaactt catcattagc gctgatttga tcaagatact cgttcagata
     5461 ccgccagttt aaatcgacat gaattttatc taccgctaac aaatcgtgat ctttgccgtt
     5521 gatacgtagc gtaaaagaga tacatttttt acccggacga tcaaagcaat tgtatgccgg
     5581 attccagctg ttgttagagt catctgaagc caccgaccac ggtcgtttcg atgggttaaa
     5641 atcggcaaaa ataacccgcc ctgactcgtc gacaagcggt ccatttactt gttcctgatg
     5701 ataaacaacg attttatcat tcgccctccg tgaaaagaga taatatttat tatcatcaag
     5761 taccagtccc accgagtcta aaaatggcat catttccatt ttcagaatca cggcgcgttg
     5821 aatctcctct gtatttttta ataaatacag tgagtccttt aatgtcaggg ccgaacgttc
     5881 aatatcttta aagaaatagc caacgttttt ctgaataagt tcaaagcggc tatcggcacg
     5941 ttttaccgcg cgttccgata ccagttgata gttaatgatg aaaaaaagac caataaatga
     6001 cagtgctgcc aataaaaata aaattatcag ccttatcttt a
//
LOCUS       DAEVZA010000208         6030 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEVZA010000208 DAEVZA010000000
VERSION     DAEVZA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.208"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1424
                     /locus_tag="LAF92_004757"
     CDS             <1..1424
                     /locus_tag="LAF92_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBI9793649.1"
                     /translation="SAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQVAPEDC
                     TGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKLERIDI
                     RTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLPSTPYT
                     TDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLTALKSD
                     ATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFGGLDHV
                     LSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMMMYGHV
                     YVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQLTATGF
                     WPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQLWKDAA
                     ADLQKRYDFLAQMAGKAEKSNTD"
     gene            1791..2924
                     /gene="ompN"
                     /locus_tag="LAF92_004758"
     CDS             1791..2924
                     /gene="ompN"
                     /locus_tag="LAF92_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBI9793650.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            3065..3499
                     /gene="uspF"
                     /locus_tag="LAF92_004759"
     CDS             3065..3499
                     /gene="uspF"
                     /locus_tag="LAF92_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBI9793651.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(3764..3937)
                     /locus_tag="LAF92_004760"
     CDS             complement(3764..3937)
                     /locus_tag="LAF92_004760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793652.1"
                     /translation="MQERAAVGLTLIETFWNFFLWQKWGKTLQKGQKRGKKRVDYRSL
                     LFSLMMFNTLKNK"
     gene            4277..4390
                     /locus_tag="LAF92_004761"
     CDS             4277..4390
                     /locus_tag="LAF92_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793653.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            4459..4692
                     /gene="ydfK"
                     /locus_tag="LAF92_004762"
     CDS             4459..4692
                     /gene="ydfK"
                     /locus_tag="LAF92_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HBI9793654.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            5009..5599
                     /locus_tag="LAF92_004763"
     CDS             5009..5599
                     /locus_tag="LAF92_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI9793655.1"
                     /translation="MSQIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(5697..>6030)
                     /locus_tag="LAF92_004764"
     CDS             complement(5697..>6030)
                     /locus_tag="LAF92_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI9793656.1"
                     /translation="GDELFISELGPLPENVTWLSPEGEFQKWNGTAWVKDTEAEKLFR
                     IREAEETKNNLMQVASEHIAPLQDAADLEIATEEEISLLEAWKKYRVLLNRVDTSTSQ
                     DIEWPALP"
BASE COUNT         1746 a         1387 c         1285 g         1612 t
ORIGIN      
        1 actcagctat tcgcgcaaaa gtggtaccgc ctgaagcgat ggaaaacgcc cctgccagcc
       61 tgcattcgct ggatgtgaaa tcgcgtgata tgcgcgggca gaaatatgtc ttgcaggtgg
      121 ctccggaaga ttgcaccggt tgtaacctgt gcgtcgaagt ttgcccggcg aaagaccgtc
      181 agaatccaga gattaaagcc atcaatatga tgtcgcgcct ggaacatgtc gaagaagaga
      241 aaatcaatta cgatttcttc ctcaacctgc cagaaatcga ccgtagcaaa ctggaacgta
      301 ttgatattcg tacatcgcag ctgattacac cgctgtttga atattcaggt gcttgctccg
      361 gttgtggcga gacgccgtat attaaattgc tgactcagct ctatggcgac cggatgttga
      421 tcgctaacgc caccggctgt tcttcaattt atggcggtaa cctgccctct acaccgtata
      481 ccaccgatgc caacggtcgt gggccggcat gggcgaactc tctgtttgaa gataatgccg
      541 aatttggcct tggtttccgc ctgacggtcg atcaacaccg tgtccgcgtg ctgcgtctgc
      601 tggatcaatt tgccgataaa atcccggcgg aattactgac agcgttgaaa tcagacgcta
      661 cgccagaggt tcgtcgagaa caggtcgcag ctttacgcca gcaactcaac gatgttgccg
      721 aagcacatga actgctgcgt gacgcagatg cactggtgga aaaatcaatc tggctgattg
      781 gtggcgacgg ctgggcttac gatatcggct ttggcggtct ggatcatgta ttgagtttga
      841 cggaaaacgt caacattctg gtgctggata cgcaatgcta ttccaacacc ggtggtcagg
      901 cgtcgaaagc gacaccgctg ggtgcagtaa ctaaatttgg cgagcacggc aaacgtaaag
      961 cgcgtaaaga tcttggcgtc agtatgatga tgtacggtca tgtttatgtg gcgcagattt
     1021 ctctcggcgc gcagctcaac cagacggtga aagcgattca ggaagcggaa gcgtatccgg
     1081 ggccatcgct gatcatcgct tatagcccat gtgaagagca tggttacgat ctggcactca
     1141 gccacgacca gatgcgccaa ctcacagcta ccggcttctg gccgctatat cgcttcgatc
     1201 cgcgtcgtgc cgatgaaggc aaactgccgc tggcgctgga ttcacgcccg ccgtcagtag
     1261 cactggaaga aacgttactt cacgagcaac gtttccgtcg gctgaattcg cagcagccag
     1321 aagtggcaga acagttatgg aaagatgctg cagctgattt gcaaaaacgc tatgacttcc
     1381 tggcacaaat ggccggaaaa gcggaaaaaa gcaacaccga ttaaatgctc tggataagga
     1441 ttatccaatt ctaaaaaaaa agcccggacg actgttcggg cttgtctttt tatatgttgg
     1501 aaaatcagtg gcaatgcaat ggcccaacag aaaataaaat ctgtgacaat aaaggcatat
     1561 aacccgcgca gaataacgta tagaaaatta aaagtataat ttttattttg tatgtattat
     1621 cttcgataca ttcaccagac ttattatcat ttcagaggaa ttatttgatt aaggttttac
     1681 ttaaggcgta acaaatgata tttatcggct aactgaactt ctcctttagg atgttttcac
     1741 tccccggaat ggggataaaa ggcaaataaa ataactaaag gatttattca atgaaaagca
     1801 aagtactggc acttttaatt cctgccctgc tcgccgcagg tgctgcacat gcagccgaag
     1861 tttataataa agacggcaac aaattagatc tgtatggcaa agttgatggc ctgcattatt
     1921 tttctgataa ttcagcgaaa gatggcgatc agagctatgc gcgtctgggt tttaaaggcg
     1981 aaacccaaat taacgatcaa ctcactggct acggtcaatg ggaatacaat attcaggcaa
     2041 acaacactga atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc gggctgaaat
     2101 ttgcagatta cggttctttc gattacggac gtaattatgg cgtaatgtac gacatcgaag
     2161 gctggaccga tatgctgcct gaatttggcg gtgactctta taccaatgca gacaacttta
     2221 tgactggtcg agccaatggc gtcgcgactt atcgtaatac tgatttcttc ggtctggtaa
     2281 atggtctgaa cttcgcggtg cagtatcaag gtaacaacga aggagccagt aatggtcagg
     2341 aaggcaccaa caacggacgt gatgttcgcc atgaaaacgg tgacggctgg ggtctttcca
     2401 caacatatga tttaggcatg ggctttagcg ctggtgcggc atacacctct tctgaccgca
     2461 ccaatgacca ggttaaccat actgcggcgg gtggtgataa agcagacgcg tggactgctg
     2521 ggctaaaata cgatgctaac aatatttacc tggcaaccat gtattcagaa acgcgtaata
     2581 tgaccccgtt tggcgacagc gattatgctg ttgcaaacaa aacccagaac tttgaagtca
     2641 ctgcacagta ccagtttgat tttggtctgc gtccggcagt ctctttcctg atgtctaaag
     2701 gccgtgacct gcacgctgcc ggtggtgcag acaacccggc aggtgttgat gataaagatc
     2761 tggttaaata cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacttatg
     2821 ttgactataa aatcaacctg ttggatgaag atgacagttt ctacgctgcc aatggcatct
     2881 ctaccgatga tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg
     2941 ttatggcggg ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc
     3001 cgtcattaca caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg
     3061 ttttatgaac agaacgattc ttgtccctat cgatatttcc gattcagaat taactcaacg
     3121 cgtgattagc cacgttgagg cagaggcaaa gattgatgat gcagaggttc atttcctgac
     3181 ggtaatacct tcactgccct actatgcctc tctgggttta gcgtattccg cagaattacc
     3241 ggcaatggat gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt
     3301 taaactgcca accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat
     3361 tctggaaatg gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga
     3421 tatcaccact tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc
     3481 cgtgctggtt gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt
     3541 cgcaaatgtg ctgacatttt cccctctaat ccgtaccata cacgccactg tttttatatc
     3601 agatttctta tgctgggcat tccggcatga cgcatactct tctgatgcca tataacgaat
     3661 tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga
     3721 acaagtaagg gcaaaaatca caactatctg actcataagt gttttactta tttttcaatg
     3781 tgttaaacat catcagcgag aacaataaac tacgataatc cactcttttt ttgccccttt
     3841 tttgcccctt ttgcagcgtt ttgccccatt tttgccacaa aaaaaaattc caaaacgtct
     3901 caatcaaagt caaaccaacc gcagcacgtt cttgcatacg acgtgactgc ggtttttcaa
     3961 ctattcagat acatcactcc catcacattc attcctccgc atcataggca tataggctat
     4021 atcaccttga tatttttctt cttcagataa aaactgttat ctatgtatac ttttaaaccc
     4081 aatccgtgta gagtctctgc ataagatagt ttgcagttgc cgcttcagct tgcgccataa
     4141 accgcctgat ttttgctgcc acctgttagc attcctgtat acctgaaacg acaatgttta
     4201 tctacgaact ttaagaacac ccaagataaa aattgtcaac tatatcatat ataacacatt
     4261 actaattcga ggctatatga acagcatact gataatcaca tctctcctta tcatattcag
     4321 catttttagt catgccctaa taaaattagg gattggcata tccaataacc cagacaaaac
     4381 cgatgtataa gtcaacatat cctgaatcag acatacaata tcgcaatgaa aatcaataat
     4441 attttaagga atatcttcat gaaatcaaaa gacaccctaa agtggttccc tgcgcagctt
     4501 cctgaagtaa gaattatcct aggggatgct gtagtggaag tagcaaaaca gggaagacct
     4561 atcaatacca gaacattgct tgattacatt gaaggaaaca taaagaaaaa atcatggctg
     4621 gataacaaag aattattaca aacagcgata tcagttctta aagacaacca aaatttaaat
     4681 ggtaaaatgt aatataataa acttactttt ttatcatttt tccactttaa caacattttg
     4741 ctccactttt ccacgaccaa acaacttgaa atctggttaa aataacacgc aacactattc
     4801 ttcttccttg agtccgcccg gaactcgaaa aacaaaccga gttaaagcca tttttcacaa
     4861 aatcgatttt gggtctcacc aaaattacgg gattgcatac gcattcgttt attttcgaac
     4921 gtgtacatac aaatatgcac aaaaataatc ataattattt tctgagatgc attatgatat
     4981 gaacaccaat ttcgtataga gtctcactat gtctcaaatt tttgcttact gtcggatatc
     5041 aacgctggat cagaccaccg aaaatcaacg ccgggaaatc gaaagtgcag gttttaaaat
     5101 caaacctcag caaataatcg aagaacacat tagtggctca gcagcaacca gtgagcgtcc
     5161 tggttttaac cggttgcttg ctcgcctgaa atgtggtgat caattgattg tgacaaaact
     5221 ggatcgcctt ggttgtaatg caatggatat caggaaaaca gtggaacaac tgaccgaaac
     5281 aggtatcaga gtgcattgct tagcattggg gggcattgac ctgaccagtc caacaggaaa
     5341 aatgatgatg caagtaattt cagcagtcgc tgaatttgaa cgagaccttt tacttgaacg
     5401 cactcattcc gggatagtaa gagcccgcgg cgcagggaaa cgttttggtc gaccacctgt
     5461 gttaaatgaa gaacagaaac aggtggtatt cgaacgaatt aagtcaggtg taagtataag
     5521 tgccattgcc cgggaattca aaacctcgcg gcaaaccatt ttaagagcca aagcaaaact
     5581 tcagacacct gacatataaa aaataatctc ggtgtgagat gctttacgtc ttccaagccc
     5641 ccttccttgc cgtaaatgga aagatacatc taattataga atttatatgt tttaccctac
     5701 ggcagtgctg gccattcaat atcctgtgaa gttgacgtat caacacggtt cagcaatacc
     5761 cgatactttt tccatgcttc cagcaacgag atttcttcct ccgttgcaat ttccagatct
     5821 gcagcatcct gaagcggcgc aatatgctca ctggctacct gcatcaggtt gttttttgtt
     5881 tcttccgcct cccggatccg gaacagtttt tctgcttccg tatccttcac ccaggctgtg
     5941 ccgttccact tctgaaactc cccttccggc gataaccagg taacattttc cggtaacgga
     6001 ccgagttcag aaataaataa ctcgtcccct
//
LOCUS       DAEVZA010000209         5954 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEVZA010000209 DAEVZA010000000
VERSION     DAEVZA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.209"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..714
                     /gene="repA"
                     /locus_tag="LAF92_004765"
     CDS             <1..714
                     /gene="repA"
                     /locus_tag="LAF92_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBI9793657.1"
                     /translation="IHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQC
                     SITTLAIECGLATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITF
                     TSALFAALDVSEEAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWSFVRERFRSY
                     QTELKSRGIKRARARRDANRERQDIVTLVKRQLTREISEGRFTASREAVKREVERRVK
                     ERMILSRNRNYSRLATASP"
     gene            1647..1991
                     /locus_tag="LAF92_004766"
     CDS             1647..1991
                     /locus_tag="LAF92_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793658.1"
                     /translation="MDLYHFTAIPMLHSILASEGLREGYLTLYDGTILYNKVWLTTSP
                     LPYGHGLCNGTEKLSESEKSFMRRVGNISESTSINGTHNKKLIRLKIDTEWIKKQPGF
                     CSYKKLMRDLDR"
     gene            complement(2172..3287)
                     /locus_tag="LAF92_004767"
                     /pseudo
     CDS             complement(2172..3287)
                     /locus_tag="LAF92_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3287..3652)
                     /locus_tag="LAF92_004768"
     CDS             complement(3287..3652)
                     /locus_tag="LAF92_004768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793659.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDAEVLRFDN
                     FDDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVTPPV"
     gene            complement(3774..4289)
                     /locus_tag="LAF92_004769"
                     /pseudo
     CDS             complement(3774..4289)
                     /locus_tag="LAF92_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            complement(4459..5658)
                     /locus_tag="LAF92_004770"
     CDS             complement(4459..5658)
                     /locus_tag="LAF92_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBI9793660.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHL
                     VFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITT
                     ESQWRSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDIS
                     LLRYMPA"
     gene            complement(5668..5856)
                     /locus_tag="LAF92_004771"
     CDS             complement(5668..5856)
                     /locus_tag="LAF92_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793661.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSI
                     LKRRGDTAALTLLQDTGV"
BASE COUNT         1440 a         1633 c         1428 g         1453 t
ORIGIN      
        1 attcatgtgg cgcatgcccg ttcgcgtggt ctgcgtcggc gcatgccacc ggtactgcgt
       61 cgccgggcca ttgatgcgct gctgcagggg ctgtgtttcc actatgaccc gctggccaac
      121 cgcgtccagt gctccatcac cacgctggcc attgagtgcg gactggcgac agagtctgct
      181 gccggaaaac tctccatcac ccgtgccacc cgggctctga cgttcctgtc agagctggga
      241 ctgattacct accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc
      301 acgttcacat ctgcactgtt tgctgccctc gatgtatcag aggaggcagt ggccgccgcg
      361 cgccgcagcc gtgttgaatg ggaaaacaga cagcgcaaaa agcagggact ggataccctg
      421 ggtatggatg agctgatagc gaaagcctgg agttttgtgc gtgagcgttt ccgcagttac
      481 cagacagagc ttaagtcccg tggaataaaa cgtgcccgtg cgcgtcgtga tgcgaacagg
      541 gaacgtcagg atatcgtcac cctggtgaaa cggcagctga cgcgtgaaat ctcggaaggg
      601 cgcttcactg ccagtcgtga ggcggtaaaa cgcgaagtgg agcgtcgtgt gaaagagcgc
      661 atgattctgt cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtga
      721 cctcctctga ataatccggc ccgcaccgga ggcatctgca cgcctgaagc ctgtcgacga
      781 acaaaaaaac agcactgcat acaaaaaaca acctaatcat ccaccttcag gtgcatccgg
      841 tccctcctgt ttttgataca aaacacgcct cacagacgag gaattttgct tatccacatt
      901 taactgcaag ggacttcccc ataaggttac aatcgttaat gtcataaagc gccagctgtc
      961 agccctacag ggtgcaatgt atcttttaaa cacctgttta tatctccttt aaactactta
     1021 attacattca tttaaaaaga aaacctattc actgcttgtc ctgtggacag acagatatgc
     1081 acctcccacc gtaaacggcg ggccccaacc ggagccactt tagttacaac acacaaaacc
     1141 aacctccaga aaaaccccga accagcgcag aactgaaacc acaaagcccc tccctcataa
     1201 ctgaaaagcg gccccgcccc ggcccttcgg gccggaacag agtcgctttt aattatgaat
     1261 gttgtaacta catcttcatc gctgtcagtc ttctcgctgg aagtattgaa tacacgctcg
     1321 taagcggccc tgacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaccctcgt
     1381 cgggaccact ccgaccgcgc acagaaactc tgtcatggct gaaagcgggt atggtctgac
     1441 agggctgggg atggataagg tgaaatctat caatcagtac cggcttacgc cgggcttcgg
     1501 cggttttact ccggtatcac atgtaaccac agcgtgccgc cttacatgcc gctgaagcgg
     1561 cattcagtaa acaggctatg cacgactcgc cagtctggcc tgaacatgat ataaaatgaa
     1621 tataaataga cagaataggg ttatttatgg atttatatca ctttacagca attcctatgt
     1681 tgcattccat acttgcatct gaaggcctga gagaaggata tctaacgttg tatgatggaa
     1741 caattttgta taataaagta tggttaacga catctccatt accatatggt cacggattat
     1801 gtaacggtac tgaaaaatta agtgaatcag aaaaatcatt tatgcgaaga gtcggtaata
     1861 tatctgagtc tacgtctata aatggaacac ataataaaaa gttaataagg ttaaaaatag
     1921 atactgaatg gataaaaaag cagcctggat tttgctcata taaaaagctg atgcgtgacc
     1981 ttgatcgata ggaatttaaa tccccaaaag actaaaaaag caccacaaaa cagatatttc
     2041 ttcaacacct cttgtgcacc atatgagcgg aaacgactat taaacaggat gacactgatt
     2101 gatacagggt atgaatggcg gttttttgct ccgtttccgg taaaatggac gcaacttccc
     2161 ctctgcgact ctcagccgca cacctgctgt cgtgccagtc gctcatgcat caggaccagc
     2221 tctgccagtc ggtaacctct gatacatccc tctccacctg atttgcatcg ccgtttttct
     2281 caccgtctcc gttatgacgt acacaacttc ttccagtaag cggcgcttca ccggactcag
     2341 gaaaccgtag tacctcacca tacggaaccc cttatctgct acatgccagg agaacctttc
     2401 catgaactca tctccactca tcaacaggta ttcttcccgt tttgtacggt gactgttgta
     2461 acgcagaccg atttcatcct gaccggcata atgctccagc cgactcatcg gcaccggtgg
     2521 ctttttcagg taagagccga agtacaccgc cacatgtgtg gcgttatcca tcaccctgga
     2581 tatgttgaca ttccagccgc ggcggtaatg cgtgtccagg aagcgattcc agtcccgttt
     2641 gctgtttcct tccactgcca gttcatccgg tattaccagc tccgggtatt tccgggacag
     2701 tagccgcgtt atccggtagc gccacatgct catcacctta cgggcgtata aatgaagatt
     2761 tttccaggtg tggcccgacg tcacaccacc ggcagttgtc gataaatgga tatgcggatg
     2821 ccactgctgg tcactccccc atgtgtggat caccgtgaat atccccggct ccacatctgc
     2881 ctgatggcag atttccagta tcacatccgc tgcaatgcgg ttcatctctg ccagtaacca
     2941 ccggttgtgg aacaccaggg accagtactg gcagggaagt gtgaacacaa tatgctgcca
     3001 cgggcagtcg gggaccaggc tcagcagata ctgtatccac tgtgcgccag ccttcacccc
     3061 gcagttcgga caggagcggc tttacaccgg aagcagatct ttttggtgtg gcaacagtcc
     3121 ggtgacgaac agcaccattg cgtgtagccc atcagcgtgg tcccgcatgc catgattttt
     3181 gtcactgact cagtcaccac cggacgcact gaaccttccg gctgtttctc cagccagtta
     3241 agccagcggt taccctgctg aaaaatatcg gcaaaacggg gaagcatcag acgggtgggg
     3301 taacgccatc cggccagtga actgtgccgc attccgggca gtacatacca ccggcactga
     3361 taccggcaag aatggttgca aactcccgct ccgtacagcg ggctatttct ggataccctt
     3421 cgtcatcgac acgaacaaac cagaagacca gctttttgtt tcctgcatcc agaaagaacg
     3481 gaatacccag gtcagcgcag cactcaacgg catcgtcaaa attatcaaag cgcagaactt
     3541 cagcgtcttc ttcgtcaaaa aaatcatccg gatgaagttt cacaacatag cggggaagtt
     3601 tgcttctttg tggtgcgggt ttgcgtttac ggggtttagc tgaacgggcc atataactac
     3661 ctgaaagaca atgacattat ctttttttat aacggtaatt ccaggccatg acaagccgca
     3721 gccgccaggc tgcctactcg agggagtctg acatcaagag cgcggttgta gattcaatct
     3781 gtcaatgcaa cacccctttc aattatctct ttcggtgttt tgaacttcag tgtctttctc
     3841 ggtctgttgt ttagctgagc agcaaccaga tctagttcat gttgagtata ttgggcaaga
     3901 catgtctttt taggaaagta ctgccgaatt agcccatttg tgttctcatt tgttccccgc
     3961 tgccaaggac tctgaggatc gcagaagtaa actttaacgc cggtgctgac agtaaattct
     4021 agatgtctgg tcagttccat tcctctgtcc catgtcagtg attttctgag ttctgacggt
     4081 aaactcagga atttgtcggt aagagcctga tttactgaga cagaatcttt gcccctgagt
     4141 ctgaggatga tcgtataacg tgattttcgg tctacaagtg tggctatatg agagtttttt
     4201 gtacctgaga ctaaatcgcc ctcccaatgc cccagagagc gtctgttatc gatatttcgg
     4261 gaacgttcgt gaattggtgt tccgttcacg tataggaaat tgaaaaacca gtcgatacca
     4321 ttgtccacgc cttatgccac gtgagccttt ctgcttttcc caccactccg gtggtttccc
     4381 tctctgtatg gtcgattttt ataccgtaac aggctgtttt ttatactttc accgattccc
     4441 gggcacactt agcctgtgtc aggccggcat atacctcagt agactgatat cccgcgcgtt
     4501 tttcttcagc cctgatacat tcagccccgc aatggcccgg cggtatacca tccggcagcc
     4561 acacagcacg cattcgaacg ggtcacgact caggaactgt ttcaccattt gtgcatagca
     4621 cactttcgcc actggttccg gtttatccat ccccagtgca cggtacacct gcggcagctt
     4681 ctctccacac acacggttgg caaggaaccc gaagtacctc accatcttaa aaaacttctc
     4741 cgggatgtgc tgtttcagcc tcgcgaccag ctcacgctgt gtcagcgttt ccgtcgccgt
     4801 ttctcccgtt ttgtggtcca ggtaacggaa gctcaggctt gcccctccgt tgtaatgagc
     4861 cagtcgggaa gccgctattg gtggcttctt cagataacga cccaggtagc gtgccgtatt
     4921 ccgccctccg gctgtcttct tcgacatgta cacatgccag tattttccgc cggatttcag
     4981 caccaggctt ctccactgtg attccgtcgt gatatgtgac aacgactccg gcattgccat
     5041 cccctctgac cacgctttca gaagcagctg ccgcatattc cacatccacc gtgaacgcat
     5101 cgcgtctttc aggaagctca gctttttcca ctgaccatgc ttattcagac ctccacaggt
     5161 tacagacaca tgtacatgcg gatgccagtt gagacgacgg ccatacgtgt ggatggcgca
     5221 gaagataccg ggttccagcc cccgttttcg ggcggcatac agcagattct ccaccgccag
     5281 acggcacacg tcattcagca gccagcggtt gctttcgaac accggccaca gcgtgtccgg
     5341 cagggtgaag accagatgta cccagtcgca gtcaggaaga cgattcagct gtgttgctgt
     5401 ccacaggtct gtggccttct ttccgcagga cgggcaggca cggctgccgc atgagttggt
     5461 caggtacttt acgtgctgac agtccgggtt atcacacccg aactctttta cacccagtat
     5521 ccgtgtgccg caggccagca ttttggtgac ggcttcaacc tcgatatcgc gcagaccgcc
     5581 cgcatccagg aaggacgtcc agcactggtt ggctgtgaac agacgtttca gagggcgggg
     5641 agtaaaaccg gacaacatga ggatggatta aaccccggta tcctgtagca gggtaagggc
     5701 tgctgtatcg ccacggcgct tcaggatgga ggtcgtcacc atcgcaggtg gtgggtccat
     5761 gggtttgagc attttcatcc agtcccattc tcgttgtgag agcttctcag caccaacaga
     5821 gagcagaact gtatcgatac ttttgcgggt caacataagg ggatgagtcc ttaataaaac
     5881 aggtcctcag agcatacata ttctgcgggg cgtgcggcaa caacaggacg aaccggggcg
     5941 gttttcaatt tgca
//
LOCUS       DAEVZA010000210         5929 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEVZA010000210 DAEVZA010000000
VERSION     DAEVZA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.210"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..886
                     /gene="narH"
                     /locus_tag="LAF92_004772"
     CDS             <1..886
                     /gene="narH"
                     /locus_tag="LAF92_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415984.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBI9793662.1"
                     /translation="LCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVG
                     RIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIE
                     AAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAI
                     ESLRIPVQYLANMLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVA
                     QVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNS
                     SRIDAINITEVRDKAEGE"
     gene            886..1581
                     /gene="narW"
                     /locus_tag="LAF92_004773"
     CDS             886..1581
                     /gene="narW"
                     /locus_tag="LAF92_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBI9793663.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            1578..2258
                     /gene="narI"
                     /locus_tag="LAF92_004774"
     CDS             1578..2258
                     /gene="narI"
                     /locus_tag="LAF92_004774"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBI9793664.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            2337..3230
                     /locus_tag="LAF92_004775"
     CDS             2337..3230
                     /locus_tag="LAF92_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBI9793665.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(3326..4171)
                     /gene="nhoA"
                     /locus_tag="LAF92_004776"
     CDS             complement(3326..4171)
                     /gene="nhoA"
                     /locus_tag="LAF92_004776"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBI9793666.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            4344..4913
                     /locus_tag="LAF92_004777"
     CDS             4344..4913
                     /locus_tag="LAF92_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBI9793667.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(4917..5144)
                     /gene="pptA"
                     /locus_tag="LAF92_004778"
     CDS             complement(4917..5144)
                     /gene="pptA"
                     /locus_tag="LAF92_004778"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HBI9793668.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(5198..5929)
                     /locus_tag="LAF92_004779"
                     /pseudo
     CDS             complement(5198..5929)
                     /locus_tag="LAF92_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1430 a         1498 c         1513 g         1488 t
ORIGIN      
        1 tttgtgcatt agcggttgtc cgtacaaaaa aatctacttc aactggaaaa gcggcaagtc
       61 agaaaaatgt atcttctgtt atccgcgaat tgagtcaggc caacccaccg tgtgctcaga
      121 aacctgcgtg ggtcgcatcc ggtatctggg cgtgctgctt tacgacgccg accgcattga
      181 ggaagcggcg agcaccgagc gcgaagttga cctctatgaa cgtcagtgcg aagtgttcct
      241 cgatccacac gatccctcag tgatcgagga agccctgaaa caaggtattc cacaaaacgt
      301 gattgaagct gcccagcgtt cgcctgtcta caaaatggcg atggactgga aactggcgct
      361 accgctgcac cctgaatatc gcaccctgcc aatggtctgg tacgttcctc cgctgtcgcc
      421 gattcagtcc tacgcagatg cgggcggttt gccgaaaagc gaaggcgtgc tgcccgccat
      481 cgaaagcctg cgtattccgg tgcaatatct cgccaatatg ttgagtgccg gcgataccgg
      541 tccggtactg cgggcgctga aacggatgat ggcgatgcgc cactatatgc gttcacaaac
      601 cgtggaaggc gttactgata ctcgtgccat cgacgaagta ggcctgagcg tcgcccaggt
      661 cgaagagatg tatcgttacc tcgccattgc caactatgaa gatcgttttg tcatcccgac
      721 gagccatcgg gaaatggcgg gcgatgcctt cgcagaacgc aacggctgcg gttttacctt
      781 tggcgacggt tgtcacggct cggacagcaa attcaacctg ttcaacagta gccgtatcga
      841 tgccatcaac atcaccgaag tgcgcgacaa agcggagggc gaataatgca gatcctcaaa
      901 gtgatcggcc tgttgatgga gtatccggat gagctgttgt gggaatgcaa ggaggacgcg
      961 ctggcgttga tccgccgcga cgcgccgatg cttacggatt tcacccacaa cctgcttaac
     1021 gcgccgctgc tggataaaca ggccgaatgg tgcgaagtgt ttgaccgcgg gcgcaccacg
     1081 tcgctgctgc tgttcgaaca tgttcatgcc gagtcccgcg atcgcggcca ggcaatggtg
     1141 gacctgctgg cggagtatga aaaggtcggc ctgcagctgg attgtcggga actgcccgat
     1201 tatctaccgc tgtatctgga gtatttaagc gtactgcctg acgatcaggc gaaagaagga
     1261 ttgctcaatg ttgcgccgat cctcgccctg cttggcggtc gcttaaaaca acgcgaggca
     1321 ccgtggtacg cgttgtttga tgctctgctg caactggcgg gaagtatact ttcaagtgac
     1381 agtgtcacaa aacaagtaaa cagcgaagag cgcgatgaca cccgccaggc gcttgatgca
     1441 gtgtgggaag aggaacaggt gaagtttatt gaagataacg ccacggcgtg tgacagctcg
     1501 ccgcttaatc aatatcagcg acgctttagc caggatgtcg cgccgcaata tgtcgacatc
     1561 agtgcgggag gtgggaaatg attcagtatc tgaacgtctt tttttacgat atctacccgt
     1621 acatttgtgc gacggtgttt ttcctcggca gctggctgcg ctacgactac ggccagtaca
     1681 cctggcgcgc ctcctcaagt caaatgctcg ataaacgcgg gatggtgata tggtcgaatc
     1741 tgttccatat cggcattttg gggattttct tcgggcacct gttcggcatg ttaacgccgc
     1801 actggatgta cgcgtggttt ttgccagtgg cagcgaaaca actcatggcg atggtgctcg
     1861 gcggtatttg cggtgtgttg acattgattg gcggcgcagg actgctgtgg cgcaggctga
     1921 caaatcagcg cgtgcgtgca acttccacta cgccggatat tatcatcatg agcattttgc
     1981 tgatccagtg tctgctgggg ctaagtacca taccgttttc agcacagtat cctgatggta
     2041 gcgaaatgat gaagctggtg ggttgggcgc aaagcattgt cactttccgt ggtggatcgt
     2101 cagaaatgct taacggcgta gcgttcgttt tccgcctgca tctggtattg ggaatgacca
     2161 tcttcctgct cttcccgttc accagattgg tgcatgtgtg gagcgcgccg tttgagtact
     2221 ttactcgtcg gtatcagatt gtgcgttcgc ggcgataatt tcaccctggc cggatagccc
     2281 ttcgctctcc ggccacttgc aggctatgat agttctccag tcacaggaga acgttaatga
     2341 aaccgcaggt gtaccacgtc gatgctttta cctctcaacc atttcgcggc aattctgccg
     2401 gtgttgtttt ccccgccgat aatcttagcg aagcgcaaat gcagcttatc gcccgcgagt
     2461 taggccattc ggaaactgct tttctgctgc acagcgacga cagcgatgtg cgcatccgct
     2521 actttacgcc aacggttgaa gtgccgattt gcggtcacgc gacagtagct gcgcactatg
     2581 tacgtgcgaa ggtgttaggt ttaggaaatt gcacgatctg gcaaacatcg ctggcaggaa
     2641 aacatcgcgt gactatcgaa aagcacaacg atgattatcg tatttcgctg gaacaaggta
     2701 cgccgggctt tgaaccgccg ctggagggtg aaacacgtga ggcgattatc aacgcgcttc
     2761 atctcactga ggacgatatt ctgccaggct tgccaattca ggtggcaaca acaggacact
     2821 caaaagtgat gatcccgttg aaaccagaag tggatatcga cgccctttcg cctgatcttg
     2881 ctgcgctgag cgctatcagc aaacagattg gctgcaatgg tttcttcccg ttccagatcc
     2941 gcccaggcaa aaacgaaacc gatggtcgca tgttctcgcc tgcgattggt attgtggaag
     3001 atccggtgac tggcaatgcc aatggcccga tgggtgcatg gttggtacat cacaacgtat
     3061 tgccccacga tggcaaggtg ctgcgtgtta aaggccatca ggggcgcgca ttggggcgtg
     3121 acggcgtgat tgaagtgacg gtgacaattc gtgataacca accggaaaaa gtcaccattt
     3181 caggcacagc ggtgattttg tttcatgccg aatgggcaat tgaaatctga aatcgttgcc
     3241 tgatgcgctt cgcctgtcag gtctacaacc gggctaatgt atgccggata aggcgattac
     3301 gccgcatccg gcaacctgac ataaattatt ttcccgcctc cggatgcgta tcaaacgccg
     3361 ccatcaccag cgctaactca tccacagtaa agccatgttt cgcatcatcc acgcccagac
     3421 caaactgttc ctgcatcacg gcatataacg acaccacatc cggtagattt cgctgctcca
     3481 ccgcgtgccc attttcataa tgggtaaaat gaaaattggt cagcgttagc ttgccaccgt
     3541 ccggcaaatg gcggcacatc agcaaatgat ggcgaaaatg cgactgcggc cagtgcgccg
     3601 accagaaatt gcccatcaca taatcgcttt gttgctgctc gcagagatca aaacggtaca
     3661 tcgactgcca atgctgatga tgattaaact gcaacaccca atcatcaccc tcctgcaaca
     3721 accgatactc tccgtgtggc gtggtctgca cgagatcgga aactaaacga atcggcgcgg
     3781 ttagcgtctg cccaccgaaa ccgacatcag caatccattt ttcctcttcc agttccacca
     3841 acagcaaacg atgggtgcgc ggcggtaatg ctggcggatt tgataacact acgcgcccta
     3901 acaagctgcg aacgttaaac cccagctcgc gtaacacccg ctcaaacacg ccattctgct
     3961 caaaacagta accgccacga cgggctatca ccagtttctc ttccagcgat tgatcatcaa
     4021 gctgtatttc cctcggcagc aaaacgtcaa ggttttcaaa cggaatggtg caattgtgtt
     4081 tcaggtgcaa tgcacgaagc gtatcaatat tgaccgcagc agctcccgac cagttaatac
     4141 gggcaaaata gtgattcaga atgggcgtca tcaggcgttt cccttagtgg ttttctcaac
     4201 ttataacctg gaatagacgc cgcaccggct attttgtgcg atcgcggaca tgcatcacgt
     4261 ttgcaaatcg ccaatcccgt taatttcgtt acttattgcg ttacggacta cgcttaaaaa
     4321 aaccttcttc gcgaggcgat accatgagcc gattcatccc catagaatta caccatgcca
     4381 gccgtctatt aaatcatggc ccaactgtca tgatcaccag tttcgatgag caatcccagc
     4441 ggcgtaacat tatggctgca gcctggtcaa tgccggtgga gtttgaaccg ccacgcgtgg
     4501 cgattgtggt agataaatcg acatggacca gggagttgat tgaacgtaac ggtaagtttg
     4561 gcatcgttat cccgggcgtt gcagcaacta actggacgtg ggcagtggga agcgtgtcgg
     4621 gtcgtgaaga agataaattt aattgctatg gcattccggt ggtgaaaggt ccggtgcttg
     4681 gtttgccact tgttgaagag aaatgcctgg cgtggatgga gtgccgcctg ctacctgcga
     4741 cttctgcgca acaaaaatac gacacgctgt ttggcgaggt ggtatcagca gcggcagacg
     4801 cacgggtatt tgtcgaaggc cgctggcagt ttgatgatga taagctcaat acgttgcatc
     4861 atttaggtgc tggcacgttt gttaccagcg gcaagcgagt tacggcgggt taagcattac
     4921 atgctgtaac cgggtttctt tatcaaagcc tccatttggg gcgcgatttc ggcatcccag
     4981 atagcttgcc aagattctgg ttgaatctgc tgtagagcaa tgcttatcga gctatcttta
     5041 ctgttcagat ggcgaataat aacgtcggta atatctgcag caagtgctgc tttttgttgt
     5101 tcgtccagtt cacgcggaaa gcatttaatg tcgatgtgcg gcatgattgt gctccttttg
     5161 aaagatgtca gtaaaattaa cacacattgt gaattttcag tcctttccat tcaaacgtga
     5221 aatcaataag ttcatcaggg acatcgcaaa caatatgaat acggatttct tctctgccgt
     5281 gactcttttc acaaattgcg taactgtcat gctctggatt atttaattct ttcagcggaa
     5341 atttttcctc aaaggcttta tttagccacc cctggtttcc tttcaccgcg aataaataat
     5401 cacctccctg tttttgtatc ttctctgcta tatctttctg gcaacccatc gcatcagttg
     5461 tgatgatttt tcctttaata tccagcatgt taagaagttc tgggatagct gtaatctcat
     5521 tagatttatc atccgtcttg atctgtccga tgaccagact gtgcattgtt gagaacgcac
     5581 taatgacatg aatcgctccc ctgcggcgac tcttatcata agaatgccgg agcgtttttc
     5641 catcaattgc aatgacgtct ttatcatctg aagaatgaca atcacgcatc cagttaataa
     5701 agcactcatg aaattttgta gggctgatac aggatacaac tctggcaata gtatcgtgaa
     5761 caggaatacc attttcaaaa tcaccatatt gcttcaagaa atcgagatgt gtttccccga
     5821 aatcctctat atcttcccaa ctttctgcac cagaaataac ggcaaaaata gtcaatagta
     5881 ggatgcctga caatttatgc tctactttcc aggcttatct gtaatcggg
//
LOCUS       DAEVZA010000211         5926 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEVZA010000211 DAEVZA010000000
VERSION     DAEVZA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.211"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..555)
                     /gene="thiK"
                     /locus_tag="LAF92_004780"
     CDS             complement(<1..555)
                     /gene="thiK"
                     /locus_tag="LAF92_004780"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBI9793669.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKVREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLI"
     gene            complement(536..1177)
                     /gene="lpoB"
                     /locus_tag="LAF92_004781"
     CDS             complement(536..1177)
                     /gene="lpoB"
                     /locus_tag="LAF92_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBI9793670.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(1191..1568)
                     /locus_tag="LAF92_004782"
     CDS             complement(1191..1568)
                     /locus_tag="LAF92_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBI9793671.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(1571..1930)
                     /gene="hinT"
                     /locus_tag="LAF92_004783"
     CDS             complement(1571..1930)
                     /gene="hinT"
                     /locus_tag="LAF92_004783"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBI9793672.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            2264..4453
                     /gene="fhuE"
                     /locus_tag="LAF92_004784"
     CDS             2264..4453
                     /gene="fhuE"
                     /locus_tag="LAF92_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBI9793673.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(4513..>5926)
                     /gene="ptsG"
                     /locus_tag="LAF92_004785"
     CDS             complement(4513..>5926)
                     /gene="ptsG"
                     /locus_tag="LAF92_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBI9793674.1"
                     /translation="NLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVVSHVMAEA
                     GGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLHLPAEEIA
                     SKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLAAIFTGVV
                     LSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPFQMQIGEY
                     TNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPENRAKVGGI
                     MISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDGTSFSHGL
                     IDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATEDAKATGTS
                     EMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVVAGSGVQA
                     IFGTKSDNLKTEMDEYIRNH"
BASE COUNT         1537 a         1560 c         1474 g         1355 t
ORIGIN      
        1 gatgagtttt aaccctgacg cgctatgcac taaatttccg gcgtggacat ccatatgcaa
       61 tggacttaag cgtaaaggcc gtggttcccg cactttgcgc agacgtttta acattcgcag
      121 ccaacccact gtccgccgcg ccggatcgct ttgctgccag tacagttcca gtaacggcaa
      181 cagcgttatt cgccagccaa aacgtggttg ttgatgtaga taatacagca agcctgccag
      241 ttcgttggta tccggcaaat acgtttttac cgcgccgggc agatagtcga ctaccatcca
      301 gtcacggaga tataaatgcg gcttcggtgc aatgcttgcg ggtagttgtg ataaagcccg
      361 atactggcgc aagaacgcgg actgcggcgc atcaggatcg tgcggctgac gcacaacaaa
      421 acgctggccc tgatgttcaa tgagaaaact cccgccacta agcccactat taaacgtcgt
      481 gacgggatga aactgcggga aaaagcgcga cagcaattcg tcgcgcatga tgggattatt
      541 gctgcgaaac ggcaccttta cctgaccaga taatttcgcc cgtctgcacc agcatcagct
      601 gcatttgtag ggtcggagcg ttaacgttgc cagaggcgct ggagtacagc acgtaatgag
      661 cgccgacatt gcgggcaatg cctatggctt tactacgggt acctaaactg tcctgcggcg
      721 acaaacctaa ctgttgctta gccatcgaca gctgctgggc ggaaaccagg gtaaatttcc
      781 cgttattagc cagcgcattt cgcagcgttt cggtcgcttc tgcggcattc agcgaaccgt
      841 tagtacggtt gttaacgcta tcaaccagca ggacgctacc cgcagtcacc ccgtcagccc
      901 caagcatctt actgaccatc ggctgcattg cgccattcca gtcataatgg cgaatatgcg
      961 gcgcaggcgg tgccgtttga tcttcgtgct caattgggcc tggctgctgc gggatcgtcg
     1021 gcaccgaggg cactgtgggg acaggctgtt gtggctcggc tggttgttcc ggcgctggtt
     1081 tcacttcttc taccggtgca ggttcacgtt gccccacaca cccggcgaga aacatcgcca
     1141 gcgcggtaat caaggcgtag cgactcattt ttgtcatcaa gattcacccc ttacaaatat
     1201 agataaagtc tgactttgtg cgcccccaga aaattggcgc tgccgtacag cgttaccgcc
     1261 gaatgtgcgg gaatgctaac gctgcgtggc ctttccagag gatgcatctc cagccctctg
     1321 gcgtcatacc agtaaaaacg ataatgaacg gtaacgggtt cttgcctttc gttataaagc
     1381 gttgaggatg ctgaaggttg aatatcagac gtcgaaagga cgggcttttc tgcactgatg
     1441 cccgcagcca ataaagatga ctccatcacc agcgattgct catcattcac cggaatttcc
     1501 ggatgtgaac gacagcccac cagcagtaac aacgccagag acaccagccc aaagcatcct
     1561 tttctcatcg ttacagacct ttatgcgcca gcattggtcc cagcggacgg ccacccaaca
     1621 agtgcatatg gatgtggtaa acctcttgtc cgccatggcg gttggtattc atgatcagac
     1681 gatagccatc ttcggcaata ccttcttgct cagcaatttt tgccgctacg gtgatcatgc
     1741 gtcccagcgc ctgctcatgc tcagctgaca cgtcgttcac agtcggaatg aggatattcg
     1801 gaatgatcag aatatgcgtt ggcgcttgcg gcgaaatatc gcgaaacgcc gttaccagat
     1861 catcctggta gacgatatcg gaggggatct cacgacgaat aattttgctg aatatagttt
     1921 cttctgccac gacattttcc tttttcaaat agccatgcgt tgcgccatgc tacgccgcgc
     1981 cacactgcga gtatagagta tggtcgagtt aaacctgctc tttcaacctt aactcacgat
     2041 ttcttaagca aaaaatcgtt ttgctgccgt atatatcgcc attattccca tttctgcctg
     2101 cgaatgcgct gaaaagacat tctctgtttg gtttacacga caaatatgaa tgcgtatatt
     2161 tctcatttgc atttacaaac aaaattattc gcacactaaa tataaccgct catcaccctc
     2221 ctccggcatg agcctgacga caacataaac caagagattt caaatgcttt caacacaatt
     2281 taacagggat aatcaatatc aagccagcac caaaccatca ctacttgccg gttgcatagc
     2341 attggcacta ttaccttctg ccgcttttgc cgctccagcc actgaagaaa cggtgattgt
     2401 tgagggttca gccacagctc cagatgatgg cgaaaatgat tacagcgtaa cgtctacctc
     2461 tgcgggtacc aaaatgcaga tgactcaacg tgatattcct cagtcggtca ctattgttag
     2521 ccagcagcgg atggaagatc agcagttaca aacgctgggc gaagtgatgg aaaacacgct
     2581 ggggatcagc aaaagtcagg cggattccga tcgtgctctt tattattccc gcggattcca
     2641 gatcgataac tatatggttg atggtatccc cacctatttt gaatcgcgct ggaatctggg
     2701 cgacgcactt tctgatatgg cactctttga acgcgtagaa gtagtgcgtg gcgcgacagg
     2761 actcatgacc gggacgggta atccatctgc ggcaattaat atggttcgaa aacacgcgac
     2821 cagtcgtgaa tttaaaggcg atgtctcggc ggaatacggt agctggaaca aagaacggta
     2881 tgtggcggat ttacaaagcc cactcaccga agacggtaaa atccgcgcgc gaattgtcgg
     2941 cggctaccag aataacgact catggctgga ccgctacaac agtgaaaaga ccttcttctc
     3001 gggcattgtc gatgctgatt taggcgatct tacgacgctt tcagccggtt acgaatatca
     3061 gcgcattgat gttaatagcc ctacctgggg cggtttaccg cgctggaata ctgatggcag
     3121 cagcaacagt tacgatcgtg cacgcagtac cgcacctgac tgggcgtaca acgataaaga
     3181 gatcaacaag gtctttatga ccctgaagca gcggtttgct gatacctggc aagcgacact
     3241 gaatgccacc cactctgaag tcgaatttga cagcaaaatg atgtatgtcg atgcctatgt
     3301 aaacaaagcg gatggtatgc tggttgggcc atacagtaat tatggacctg gctttgatta
     3361 tgtcggcggc accggttgga acagtggcaa acgtaaagtt gatgcgctgg atttgttcgc
     3421 tgacggtagt tatgaattgt ttggtcgtca gcacaatcta atgtttggtg gcagttacag
     3481 caaacaaaac aatcgttact tcagttcatg ggccaacatc ttcccggatg aaattggcag
     3541 tttctacaac tttaatggca atttcccaca aaccgactgg tcaccacaga gcctggcgca
     3601 ggacgatacc acacatatga aatcgttata tgctgccact cgtgtcaccc ttgccgatcc
     3661 gctgcatctg atcctcggcg cacgttatac caactggcgg gttgatacgc tgacttacag
     3721 catggagaaa aaccacacca cgccttacgc tggtctggtg tttgacatca atgacaactg
     3781 gtcgacctac gccagctata cctctatttt ccagccgcaa aatgatcgtg acagttcagg
     3841 caaatatctg gctccaatca ccggtaacaa ctacgagctg ggtctgaaat cggactggat
     3901 gaatagccgt ctgaccacca cattagccat cttccgtatt gagcaggata atgtcgctca
     3961 gtccaccggt acacctatcc ccggcagcaa cggcgaaacc gcctataaag cggtggatgg
     4021 gacagtcagt aaaggggtgg aatttgaact caacggcgca attaccgaca actggcagct
     4081 gacatttggc gcaacgcgct atattgcaga ggataacgaa ggaaacgccg ttaatcctaa
     4141 tctgccacgc accacggtta aaatgttcac cagctatcgg ttgcctgtca tgccagagtt
     4201 gacagtcggc ggtggtgtta actggcaaaa tcgcgtgtat accgacaccg tgacaccgta
     4261 tggcaccttc cgcgccgagc aaggtagcta cgcgctggtg gatctcttca cccgctacca
     4321 ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac ctgttcgaca aaacctacga
     4381 taccaacgtg gaaggttcta tcgtctacgg cgcaccgcgt aatttcagca ttaccggcac
     4441 gtatcaattc tgaataacac ctgtaaaaaa ggcagccatc tggctgcctt agtctcccca
     4501 acgtcttacg gattagtggt tacggatgta ctcatccatc tcggttttca ggttatcgga
     4561 tttagtaccg aaaatcgcct gaacaccaga accagcaacc actacgcccg ctgcgcccag
     4621 tttcttcagg ccggcctgat ccactttaga cacatcagca acgctgacgc gcagacgggt
     4681 aatacatgcg tcgaggttag taatgttttc tttaccacca aatgcagcaa ccagagccgg
     4741 tgccatttcg ctggtacctg tcgcttttgc atcttcagtc gcgtcttcac gacccggcgt
     4801 tttcagatcc agtgctttaa tcagcacgcg gaagatggtg tagtaaacaa tcgcataacc
     4861 gataccgacg atcgggaaca gccacagttt gctgctgtta ccagacagaa cgatgaagtc
     4921 gatcagaccg tgcgagaacg acgtaccgtc acgcatcccc agaagaatac agattgggaa
     4981 tgccaggcct gccagaatcg cgtggatgat gtacaggatc ggcgcaacga acatgaagga
     5041 gaactcgatc ggctcggtga taccggtcag gaacgaggtc agcgccgcgg aaatcataat
     5101 accgcccact ttcgcgcggt tttctggttt agcagagtgc cagatagcaa ttgcggcagc
     5161 tggcagaccg tacattttga acaggaagcc accagacagt ttacccgcag tcgggtcacc
     5221 cgccatataa cgcggaatgt cgccgtggaa aacctgacct gctgcgttgg tgtattcacc
     5281 aatctgcatc tggaaaggta cgttccagat gtggtgcaga ccaaacggta ccaggcaacg
     5341 ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc tggtaagcag cccactgaga
     5401 gaaggtctgg attgcagaac caatcggcgg ccaaatgaag gacagcacaa cgccagtaaa
     5461 gatggcagcc aggccagaaa tgatcggcac aaagcgttta ccggcaaaga agccaagata
     5521 ctcaggcagc ttaatacggt agaaacggtt aaacatgtac gctgcgatcg caccggagat
     5581 aatccctccg agtacgccag tatccgccag gtgtttagag gcgatttctt cagcaggtaa
     5641 atgcagtacc agtggcgcaa ccacggccat ggttttaacc atgatgccat aggcaacaac
     5701 tgcggccagc gcggatacgc catcgttatt ggtaaagccg agggcgacac cgatcgcaaa
     5761 aatcagtggc atgtttgcaa agacggaacc gcctgcttct gccataacat gcgatacaac
     5821 ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc agaatacctg cgataggcag
     5881 tacggatacc ggcagcatca gcgatttacc gaccttttgc aggtta
//
LOCUS       DAEVZA010000212         5909 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEVZA010000212 DAEVZA010000000
VERSION     DAEVZA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.212"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..317
                     /locus_tag="LAF92_004786"
     CDS             <1..317
                     /locus_tag="LAF92_004786"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="catalyzes the second step in the glutathione
                     biosynthesis pathway, where it synthesizes ATP +
                     gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate +
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBI9793675.1"
                     /translation="VVDGEPVPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIAR
                     QIGPTLKEKGLIFVGLDIIGDRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQ
                     QQ"
     gene            426..989
                     /locus_tag="LAF92_004787"
     CDS             426..989
                     /locus_tag="LAF92_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBI9793676.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            989..1405
                     /gene="ruvX"
                     /locus_tag="LAF92_004788"
     CDS             989..1405
                     /gene="ruvX"
                     /locus_tag="LAF92_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBI9793677.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSAAAVIILESYFEQGY"
     gene            complement(1581..2561)
                     /locus_tag="LAF92_004789"
     CDS             complement(1581..2561)
                     /locus_tag="LAF92_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBI9793678.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            2579..3283
                     /gene="yggS"
                     /locus_tag="LAF92_004790"
     CDS             2579..3283
                     /gene="yggS"
                     /locus_tag="LAF92_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBI9793679.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            3301..3867
                     /gene="yggT"
                     /locus_tag="LAF92_004791"
     CDS             3301..3867
                     /gene="yggT"
                     /locus_tag="LAF92_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBI9793680.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            3864..4154
                     /locus_tag="LAF92_004792"
     CDS             3864..4154
                     /locus_tag="LAF92_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBI9793681.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            4162..4755
                     /locus_tag="LAF92_004793"
     CDS             4162..4755
                     /locus_tag="LAF92_004793"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBI9793682.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDNQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            4748..5884
                     /gene="hemW"
                     /locus_tag="LAF92_004794"
     CDS             4748..5884
                     /gene="hemW"
                     /locus_tag="LAF92_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBI9793683.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         1386 a         1559 c         1557 g         1407 t
ORIGIN      
        1 tggtggtgga tggcgagccg gtaccgtact gcctggcgcg tattccgcag gggggcgaaa
       61 cccgtggcaa tctggctgcc ggtggacgcg gtgaacctcg tccgctgacg gaaagtgact
      121 ggaaaatcgc ccgtcagatc gggccgacgc tgaaagaaaa agggctgatt tttgttggtc
      181 tggatatcat cggcgaccgt ctgactgaaa ttaacgtcac cagcccaacc tgtattcgtg
      241 agattgaagc agagtttccg gtgtcgatca ccggaatgtt aatggatgcc atcgaagcac
      301 gtttacagca gcagtaaccc accttagcga gaaggatctc gttgagactc tgagtgacag
      361 cgcccttctt tccacgcata ctgggcgctg ttgctttttt gaaccaggaa acagaacctc
      421 tgacaatgaa tttacagcat cactttctta ttgccatgcc tgctctccag gatccgattt
      481 tccgtcgttc cgtggtctac atttgcgaac ataataccaa tggtgcaatg gggatcatcg
      541 tcaacaagcc gctggaaaat ctcaaaattg aagggattct ggaaaagctg aagatcacgc
      601 cggagccgcg tgatgaatca atccgtctgg ataaaccggt tatgctcggc ggtccgctgg
      661 ctgaagatcg cgggtttatt ttgcatactc cgccctccaa ttttgcttcc agcattcgca
      721 tttcagacaa cacagtgatg accacttccc gcgatgtgct ggaaacgctc ggcaccgata
      781 aacaaccgtc tgacgtattg gtggctctgg gttatgcctc ctgggaaaaa ggtcaactgg
      841 aacaagaaat tctcgataac gcgtggctaa cggccccggc ggatctgaat attctgttca
      901 aaacaccgat tgccgaccgc tggcgcgagg cggcaaaact gattggtgtg gatattctca
      961 ccatgcctgg tgtggcaggg cacgcctgat gagtggaacc ttactcgcct tcgacttcgg
     1021 taccaaaagc attggtgtag cggtcggcca acgcattacc ggcaccgctc gccctttgcc
     1081 tgcaattaaa gcacaggacg gtacgccgga ctggaacatt atcgagcgtt tactgaaaga
     1141 gtggcagccg gacgaaatca tcgtcggttt gccgctgaat atggacggca ccgagcaacc
     1201 attgactgcc agagcgcgta aatttgccaa ccgtattcat ggtcgtttcg gtgttgaagt
     1261 aaagctccat gacgaacgtc ttagcaccgt ggaagcccgt tccggtctgt ttgaacaggg
     1321 cggctatcgg gcgctcaaca aaggcaaagt cgactctgcc gctgcggtta ttattctcga
     1381 aagctatttc gagcagggat attaaggcga tctaaaagcc tggcggatag tcgttaccgc
     1441 catcttgccg gatgcggcgt gaacgcctta tccagcacac atctggcagc ggctgtaggt
     1501 ctgataagac gcgttagcgt cgcatcagac atttattgcc tttataggcc ctataaactt
     1561 gcgcatcggg catggcaacg tcacaaacgc ccttccccca cccgctgctg ataactctgc
     1621 tgaaacgtta tcatccccac ctgctgcccg gtttgaataa catgcggtaa ctggtgggtt
     1681 ttcccttcgc gaatcaaatt ccccaccgcg ggagtgttaa tcaataattc aaacagcgcc
     1741 acgcgtcctt cctgtttatc cacttccagc ttttgtgaca acacggcccg taaactacct
     1801 gccagttgat tacgtacggg atctttttcc tgcgccggaa atgaatccac cagtcgctca
     1861 actgcctgcg cggcaccacg cgtatgtaat gttgccagca ccaaatgccc ggtttctgcc
     1921 gccgtcagtg ccagacggat tgtctcgctg tcacgcagct ctccgagcaa aatcacatca
     1981 ggatcttccc gcaatgcggc ccgcaatccc gatgcgaacg tcatacagtg caaaccaatt
     2041 tcccgctgct ggatcaaaca tcgctggctg gcatagatat attcaacagg atcttccagc
     2101 gtcagaatat gcgcatcggc atgttgattg agatagccaa ccatcgccgc cagcgtggta
     2161 gatttgccgc tccccgtcgc ccccgtcacc agaatcaggc cattctcgct cttgagtaat
     2221 tccggcaata ccgttggtgc gccaagctgt tcgagctgtg gacagtgcga aggtaacaac
     2281 cgtaatgcca gagaaatacc ttgccgttgc gcgaacgcac tgccacgcaa ccgctggttt
     2341 tccgccagcg acacggcaaa atccagctgg ccattttcca gcaatattgc ccgctgatcg
     2401 tcatccagcc actcccgcag tagctcttcg acgtccggcg catcaaacgg cgcagcttcc
     2461 attagcccgc gaatgcgcca tcgtgcgggc caggcgctgc acaggtgtag atccgagacg
     2521 ttatgcttta cactaagggc cacaatttct tccatattca tactaagatc ctcggaaaat
     2581 gaacgatatt gcgcataacc tggcacaggt ccgggacaaa atctcagcgg ctgcaacgcg
     2641 ttgcggccgt tctccagaag aaattacgct gcttgcagtc agtaaaacaa aacctgcgag
     2701 cgccatcgca gaagccattg atgccgggca gcgtcaattt ggtgaaaact acgttcagga
     2761 aggggtagat aaaattcgcc actttcagga actgggcgta acaggattag aatggcattt
     2821 tattggcccg ttgcagtcta ataaaagccg cctggtggca gagcatttcg actggtgtca
     2881 taccatcgac cgtttgcgca ttgctacccg cctcaacgat cagcgcccgg cagaacttcc
     2941 ccctcttaac gtactgattc aaattaatat tagtgatgaa aacagtaagt ccgggattca
     3001 actggcagaa ctggacgagc tggcagcggc ggtcgctgaa ctaccgcgtt tacgtctgcg
     3061 cgggctgatg gcaatccctg cgcctgagtc agaatatgta aggcagtttg aagttgcacg
     3121 ccaaatggct gtagcatttg ccggactgaa aacgcgctac ccgcatatcg acacgctctc
     3181 tctgggaatg tcggacgata tggaagccgc cattgcggca ggtagcacga tggttcgtat
     3241 cggcactgca atttttggtg cgcgtgatta ctctaaaaaa taaggaatta aaggaacgcc
     3301 atgaatacgt tgactttcct gctttcaacg gtcattgagc tgtataccat ggtgctgtta
     3361 ttacgcatct ggatgcagtg ggctcattgt gatttttaca accccttctc acagtttgta
     3421 gtgaaggtaa cacagccaat tatcgggcca ctgcgccgcg ttattccggc aatgggtccc
     3481 attgatagcg cctcgctgct ggttgcgtac attctcagtt ttatcaaagc catcgtgctg
     3541 tttaaagtgg tgaccttcct gccaatcatc tggattgccg gtttactgat tctgctgaaa
     3601 accatcggcc tgctgatttt ctgggtcctg ctggtgatgg cgattatgag ctgggtaagc
     3661 cagggtcgta gtccgattga atacgtgctg attcagctgg ccgatccgct gctgcgcccg
     3721 attcgccgcc tgctaccggc aatgggtggg attgatttct cgccgatgat cctcgttctg
     3781 ctgctgtatg tcatcaatat gggtgtcgca gaagtattac aggcaaccgg aaacatgctg
     3841 ctgccggggc tgtggatggc gttatgagtg ccgtaacagt taatgatgac ggtctggttt
     3901 tacggcttta tattcagccg aaagccagcc gtgattctat tgtcggttta catggcgacg
     3961 aagttaaagt cgccattacc gcgccgccgg ttgacggtca ggccaacagc catctggtga
     4021 agtttctcgg taagcaattc cgggttgcca aaagccaggt ggtgattgaa aaaggcgaac
     4081 ttggccgcca caaacaaatt aaaatcatta atccgcaaca aatcccgcca gaaatcgcgg
     4141 cgttaattaa ttaggtatcc tatgcaaaaa gttgtcctcg caaccggcaa tgccggtaaa
     4201 gtgcgtgagc tggcgtcgct gcttagcgac ttcggtcttg atatcgtggc ccaaacagac
     4261 ctcggcgtag attccgctga agaaaccggc ctgaccttta tcgaaaacgc gattctgaaa
     4321 gcgcgccatg cggcaaaagt gaccggttta ccggcaattg ccgacgactc tggtctggcg
     4381 gtagatgtac ttggcggcgc gccgggcatt tactccgcgc gttattccgg tgaagacgcg
     4441 accgatcaaa agaatctgca aaaactgctg gaaacactaa aagacgtacc ggacaaccaa
     4501 cgtcaggcgc gtttccactg cgtactggta tatctgcgtc acgcggaaga tcccactccg
     4561 ctggtgtgcc acggcagctg gccgggcatg attactcgtg aaccagcggg tactggcggc
     4621 tttggttatg atccaatctt cttcgtacct tccgaaggga aaaccgctgc cgaactgacc
     4681 cgcgaagaaa agagcgccat ttcccaccgt ggtcaggcat tgaaactgct gctggacgct
     4741 ttacgtaatg gttaaattac cgccgctaag tctctacatt cacatcccgt ggtgcgtgca
     4801 gaaatgcccg tactgcgatt tcaactctca cgcgttgaaa ggagaagtgc cgcacgacga
     4861 ttatgttcag catctgctta acgacctgga caacgatgtg gcttacgctc agggccgtga
     4921 agtaaagaca atctttattg gcggtggtac gccgagcctg ctttccggcc cggcgatgca
     4981 aacgctgctg gacggcgtgc gtgcgcgttt gccgctggca gcggatgcag aaattactat
     5041 ggaagcgaac ccaggcacgg tagaagcgga tcgctttgtc gattatcagc gtgctggtgt
     5101 gaaccgcatc tctattggtg tgcagagttt tagcgaagaa aagctgaaac gacttgggcg
     5161 tattcatggc ccgcaagaag cgaaacgagc ggcaaagctg gcgagcggtt tagggttacg
     5221 tagctttaac cttgatttga tgcatggact accggatcaa tcactggaag aggcgcttgg
     5281 cgatctacgc caggccattg aactgaatcc gccgcatctt tcctggtatc aactgaccat
     5341 cgaacccaat acgctgtttg gttcgcgacc accggtgctg ccggacgacg atgcgttgtg
     5401 ggatattttc gaacaggggc atcagttatt aaccgcagcg ggttatcagc aatatgaaac
     5461 ttccgcttac gccaaacccg gttatcagtg ccagcacaat ctcaactact ggcgctttgg
     5521 cgactacatc ggtattggct gcggcgcgca cgggaaagtg accttcccgg atgggcgcat
     5581 tctgcgtacc actaaaacgc gtcatccgcg tggttttatg caaggaaggt atctggaaag
     5641 ccagcgtgat gtcgaagccg cagataagcc gtttgagttc tttatgaatc gcttccgtct
     5701 gctggaggcc gcgccgcgcg tggagtttat tgcgtatacc gggctttgcg aagatgtgat
     5761 tcgcccacag ttagacgagg cgattgccca gggttatctc accgaatgtg cggattactg
     5821 gcagataacg gaacatggta aattattctt aaactcactt cttgaattat tcctcgctga
     5881 gtaatcatta atgcgtctgc gggcgcatt
//
LOCUS       DAEVZA010000213         5824 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEVZA010000213 DAEVZA010000000
VERSION     DAEVZA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.213"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..477)
                     /locus_tag="LAF92_004795"
     CDS             complement(<1..477)
                     /locus_tag="LAF92_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBI9793684.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATR"
     gene            complement(547..1332)
                     /locus_tag="LAF92_004796"
     CDS             complement(547..1332)
                     /locus_tag="LAF92_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI9793685.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            1651..2928
                     /locus_tag="LAF92_004797"
     CDS             1651..2928
                     /locus_tag="LAF92_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9793686.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2955..4433
                     /locus_tag="LAF92_004798"
     CDS             2955..4433
                     /locus_tag="LAF92_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBI9793687.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
     repeat_region   5072..5823
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5133..5152
                     /rpt_unit_seq="cggtttatccccgctggcgc"
BASE COUNT         1517 a         1371 c         1399 g         1537 t
ORIGIN      
        1 gcgggttgct accaggccgc ccatctgcgc cagcggcttt gactgcggag aatgccccgt
       61 ggtgtaacct ttttcacgca acgcattttc cagcacctcc agcggaacgc cacattgcgc
      121 cgtcgcctgc atattctcaa tatcaatatt aatgatttga ttcatggcgg agccgtcgag
      181 caccaccgag ttttccacaa cggtttccag cccaccttca gtggcggaag cgccggtacg
      241 cggcacaccg ttaattttgt gcgcattcat aaaattcagc acacgggaga cttgttctgt
      301 ggaaccgagt tttacgaccg ctgccggaat cggcaaagta taaataccat gaatatccgg
      361 aaatttacga aaacggtcaa tactgttctt ctttaatact gtttcatcgg taattacgcg
      421 atctgcacca acaatttcct ttagctggtc gacaatcgct gcgcgagata aagacatagt
      481 aattccttcc tgataataaa aatggaataa tcattaataa gagaaaacag aaaccaccgt
      541 tatggattag cgcactaaat aaccaccatc gaccactaat aaatgcccat tgacataatt
      601 tgaagccgga cttgcgagga atacagctgc gcccattaaa tcctgagtat cgccccaacg
      661 gtttgccgga atatgatcaa gaacgcgctg attggtttct ggattactgc gtgtcgccag
      721 cgtaatatct gttgcataat agccaggggc gataccattt acctgaatat tatattgacc
      781 tagctcatca caataagctt tggtgaaccc ggcaagagca tgtttagtgg cagaatatgc
      841 aggtgaccat tgtccaccta agtaagagaa caatgaacag atattaataa ttttgccgct
      901 tttctgcggg atcataattt ttgcagcttc atagcttaac tcgaatgcgg cggtcaggtt
      961 cacatcaatc atcggatccc agtcggcacg accgaagtcc agcaccttat tcagcttaca
     1021 aataccggca ttgtttacca gaatatcaac tgtaccgaaa cgctcacagc aagcagcgat
     1081 aatcttctgc ggcgcgcctt ctgcggtgat atccacctgc atgaagtcca cctcaacacc
     1141 ctgtttttca atcatttcct ttgtttcgcc gttatctttg acgaaactag gaataaagat
     1201 atttgcgcca gctttggcca acgccatggc aaatgcctgg cctaaaccgc tattcccacc
     1261 ggtaacaatt gcggttttac ctttcagtga gaaaaaatcc attgagaacg cattgagaga
     1321 ttcgattgac atagttagct ccaatttccg taaggcaaaa aaaaagaaag gtaacttccc
     1381 ccgcgagagg gaagttacct ttctcattat ctctggtaac tggagataat ttttagcata
     1441 acggtataac tgctaatgtg atcgtaatca cagtgtgata ttcgtattgt caaatagttt
     1501 taaaaagcag aagcgtgatt actctcacac aacacctctg ctttccatgt tatttcttat
     1561 tgcaattact agagacctga gaaaagtaat ccacctgaaa gggcggctta cttttctctt
     1621 ttttttgtct attacaaatg ggaatacgat atgcaacaca actcatatcg ccgttggata
     1681 accctcgcga taattagttt tagcggcggc gttagtttcg acctggctta tttacgttat
     1741 atttatcaaa ttcccatggc gaaatttatg ggattcagca ataccgagat aggtttaata
     1801 atgagtacct ttggtattgc ggctattatt ctttatgccc ccagcggcgt tattgccgat
     1861 aaattttcac accgcaaaat gattacttcc gcgatgatca ttaccggatt actgggtctg
     1921 ttaatggcaa cgtatccacc gctgtgggta atgctctgta ttcaggtcgc ctttgcgata
     1981 acgacgattt taatgctgtg gtcggtgtcg attaaagccg cctcgttgct gggcgatcat
     2041 agtgagcaag ggaaaattat gggctggatg gaagggctgc gcggcgtcgg tgtaatgtcg
     2101 ctggcggtgt ttaccatgtg ggtcttttct cgctttgcac cggatgacag cgccagcctg
     2161 aaaacagtca ttatcatcta cagtgtggtt tacatcttgt tggggattct gtgctggttt
     2221 tttgttagcg ataacaacaa cctgcgcagt gccaataacg aagaaaaaca gtcattccag
     2281 cttagcgaca tccttgccgt tttgcgtatc agcaccacct ggtattgcag catggtgatt
     2341 tttggtgtct tcacaatcta cgccattctg agttactcca ccaactatct gaccgaaatg
     2401 tacggcatgt cgctggtggc agcgagctac atggggattg tgatcaacaa aatcttccgc
     2461 gcgctgtgcg gcccacttgg cggcattatc accacctaca gtaaagtgaa atcccctacc
     2521 cgcgtgatcc aaatcctttc cgtactcggc ctgctggcgt taactgccct gctcgtcacg
     2581 aactctaacc cgcaatcggt cgcgatgggg attggcctga ttttactgct gggattcacc
     2641 tgttacgcct cacgcgggct gtactgggcc tgccctggcg aagcgagaac accgtcttac
     2701 attatgggca ccacggtagg tatttgttcg gtgattggat tcctgccgga tgtcttcgtt
     2761 tacccgatta tcggccactg gcaagacacc ctgcccgcag cagaagccta ccgcaatatg
     2821 tggctgatgg gaatggcggc gcttgccatg gtgattgtct ttaccttttt gctgttccaa
     2881 aaaattcgta ctgctgatag cgcccccgca atggctagca gcaagtaagc caggaaaatt
     2941 acaggagaaa aatcatgtcg aaaaaataca tcatagggat tgatggcgga agtcagagca
     3001 caaaagtggt gatgtacgat ctggaaggta acgtggtttg cgaaggcaaa ggcttattac
     3061 agccgatgca cacgccagat gccgatactg cagaacatcc tgacgacgat ttatgggcat
     3121 cattatgttt tgccggtcac gatttgatga gtcagtttgc cgggaataaa gaagatattg
     3181 tcggtattgg tctgggatcc atccgttgct gtcgtgcgtt attgaaagcc gatggcacgc
     3241 ctgctgcgcc gttgattagc tggcaggatg cacgcgttac acgcccttac gaacacacta
     3301 atcctgacgt ggcatatgtc acctcttttt cgggttatct gacgcatcgc ttaaccggcg
     3361 agtttaaaga caatatcgcc aactattttg gtcagtggcc ggtggattat aagagctgga
     3421 catggagcga agatgctgcg gtaatggata agtttaatat cccccgtcat atgctgtttg
     3481 atgtgcaaat gcctggcacc gtcctcggac atatcacacc acaagccgca ctggcgacac
     3541 atttcccggc aggactgccg gttgtttgta ccaccagtga taaaccggta gaagctctgg
     3601 gggccggatt actggatgat gaaactgcgg taatttctct tggcacttac atcgcattga
     3661 tgatgaacgg caaagcactg ccgaaagatc cggtggcgta ctggccgatt atgtcttcta
     3721 ttccgcaaac attgctgtat gaaggttacg gtattcgcaa aggtatgtgg acggtgagct
     3781 ggctgcgtga catgttaggc gagtcgttaa ttcaggatgc cagggcgcag gatctttcac
     3841 cggaagattt actcaacaag aaagcctcta gtgtgccacc tggctgtaat gggctgatga
     3901 cggtgctgga ctggctgacc aatccgtggg aaccgtacaa acgcgggatt atgatcggct
     3961 ttgattccag catggattac gcatggatat atcgttcgat attggaaagc gtggcgctaa
     4021 cgctgaagaa caattacgac aatatgtgta atgaaatgaa tcactttgcg aagcatgtga
     4081 tcattactgg cggcggttcg aacagcgatc tgtttatgca aatttttgcc gacgtgttca
     4141 accttccggc acgccgtaac gccattaacg gttgtgcaag tctgggagca gcgattaata
     4201 cagcggtagg tctggggcta tacccggatt acgcaacggc tgttgataag atggttcgcg
     4261 tgaaagatat ctttataccg attgagagca atgccaaacg ctacgacgcg atgaataaag
     4321 gcattttcaa agacctaacc aaacatactg atgtgatcct gaaaaaatcg tatgaagtga
     4381 tgcatgggga attggggaat gtggattcga tccagagctg gtcgaatgcg taagtgagga
     4441 aggccgggcg ggaaactgcc cggcctgaac atacctgaat ggttatcccc gctgacgcgg
     4501 ggaacataag ttcgacaact taaaaaatga taacaagtcc ggtttatccc cgctgatgca
     4561 gggaacataa taaaaacttt aactagttcg gtttatccct gtttttacaa ggaatattaa
     4621 ctacactcga tgtctttaga ataaaagaca atacgaatcg cactttatca attttcactt
     4681 aaagtatgaa ctggatatct ttattcataa gaactattca tcaacagcgt atagaggcgg
     4741 ttatgtaaaa ccactcatta gcctcaaaac aaagacaaac ttccagattt attacttaac
     4801 caatgaataa caagcttaaa aaacatactg ttattctaaa gaaaaaatta acaggcatta
     4861 attcaatatt aaaaaaataa tagattaaaa tttcttaacg atttaagaat catacaaata
     4921 acactttgat taaatttaat tttttgtatc gataattgtg aacctctctg gcatggagaa
     4981 ctattttgaa catgaggtgt tacgtggata tgttgcttat tacaagtact gctaatataa
     5041 aaacttgaga aagagataac gggttatatg gtggtttatc cccgctggcg cggggaactc
     5101 aacatcggaa acggcttcgc ggcggcggcg tccggtttat ccccgctggc gcggggagct
     5161 ctacgtgaag aatatttgca acacccgcaa gaacggttta tccccgctgg cgcggggagc
     5221 tcggcatagc caggctgatc cggcgacggc cttacggttt atccccgctg gcgcggggaa
     5281 cacgcccgcc tcgtcggtgt attccgcgag atcgccggtt tatccccgct ggcgcgggga
     5341 acacgttatc accgagtgca gtagcccgcg cgcgctcggt ttatccccgc tggcgcgggg
     5401 aacacgtcgc cgggttgatt ttccatgatg atttttacgg tttatccccg ctggcgcggg
     5461 gaacacatat aaatcgcaaa gctcggaaaa tgttttaacg gtttatcccc gctggcgcgg
     5521 ggaacaccct ctcttatttt ttcatcgtcc atattcatgc ggtttatccc cgctggcgcg
     5581 gggaacacgg ccccggaaca tctgccgcag tgcgataccc cggtttatcc ccgctggcgc
     5641 ggggaacacg gattgcaggc atcaggtgat atcgccaaaa ccggtttatc cccgctggcg
     5701 cggggaacac tgcgttgcta tgcagatcgc gcagcgtccc gacggtttat ccccgctggc
     5761 gcggggaaca cggtaaccgt tcgttaaatt ccacgcccgc gagcggttta tccccgctgg
     5821 cgcg
//
LOCUS       DAEVZA010000214         5791 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEVZA010000214 DAEVZA010000000
VERSION     DAEVZA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.214"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..356)
                     /gene="trbB"
                     /locus_tag="LAF92_004799"
     CDS             complement(<1..356)
                     /gene="trbB"
                     /locus_tag="LAF92_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbB"
                     /protein_id="HBI9793688.1"
                     /translation="MSLIKSLLFTLLLAAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEA"
     gene            complement(343..627)
                     /gene="traQ"
                     /locus_tag="LAF92_004800"
     CDS             complement(343..627)
                     /gene="traQ"
                     /locus_tag="LAF92_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBI9793689.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGRHVSH"
     gene            complement(746..1093)
                     /gene="trbA"
                     /locus_tag="LAF92_004801"
     CDS             complement(746..1093)
                     /gene="trbA"
                     /locus_tag="LAF92_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HBI9793690.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYFFSERNDREMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDGFRRIFICIMLSSVWS
                     GVMWFFIRGLMTG"
     gene            complement(1109..1852)
                     /gene="traF"
                     /locus_tag="LAF92_004802"
     CDS             complement(1109..1852)
                     /gene="traF"
                     /locus_tag="LAF92_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBI9793691.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQVQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(1845..2102)
                     /gene="trbE"
                     /locus_tag="LAF92_004803"
     CDS             complement(1845..2102)
                     /gene="trbE"
                     /locus_tag="LAF92_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HBI9793692.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEIL
                     AAAFVIFYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(2129..3937)
                     /gene="traN"
                     /locus_tag="LAF92_004804"
     CDS             complement(2129..3937)
                     /gene="traN"
                     /locus_tag="LAF92_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBI9793693.1"
                     /translation="MKRILPLILALVAGMALADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFRRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(3934..4572)
                     /gene="trbC"
                     /locus_tag="LAF92_004805"
     CDS             complement(3934..4572)
                     /gene="trbC"
                     /locus_tag="LAF92_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBI9793694.1"
                     /translation="MNWKTFALFALTAGMHITVHASDNVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(4600..5052)
                     /locus_tag="LAF92_004806"
     CDS             complement(4600..5052)
                     /locus_tag="LAF92_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793695.1"
                     /translation="MSNNIKYGSRILDMGVLLEMKALVNANGGVSRCIMECRQLACEV
                     RHSQQLLDKLSCIDDWLCRFSSIMYHRFDKEQIQWREDVDRDYPREKVYIFSPDNVSV
                     HNNDIKDSANNTSGITVSITASKICAGQKEFDAFLKLAEKLRENPTGN"
     gene            complement(5082..5543)
                     /locus_tag="LAF92_004807"
     CDS             complement(5082..5543)
                     /locus_tag="LAF92_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI9793696.1"
                     /translation="MYKQATELMLNFKDRILIKGEEDTGKSTLLTEIRISDSDSRYYN
                     FKTLNSAGYNRLCDENIDNFDFLNTPEKTLILDGVRLCEKKMTSKVIRLIKQARKYHK
                     RLVVVADSCESEFIELLFDGVIALSFNSDRERSCNVYTPSRHRNTDNIYAR"
     gene            complement(5569..>5791)
                     /locus_tag="LAF92_004808"
     CDS             complement(5569..>5791)
                     /locus_tag="LAF92_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HBI9793697.1"
                     /translation="ETIGKNNAVCNEYPSPILPKERWRYQMVNMYPDSGQCHPFGRSV
                     TRWETGKNPPNTKKNFGYLMWRKRNCVFL"
BASE COUNT         1401 a         1549 c         1285 g         1556 t
ORIGIN      
        1 aatgcctccg gaaaggcggt atccccctga ccatccaggg tgtagggaaa aacggaaaag
       61 ccgtactgct gtgccagctg tttcagtacc gggtcaaact ggtgacagta agggcaatgc
      121 ccctgcataa acagcaccac cttccagtcg gccaggttaa cctgcctgcc gttgctgagg
      181 cggaaccagc ggggggctgg cttttctgtc ctggctgacg tgcctgctgc cggttgtgca
      241 ggctgcgcgg ccatgccctg cggattccag agtcgttcaa tttcatcccg ggtggaggcc
      301 tgcacggcag cggctgccag caacagggtg aacaacagtg atttaatgag agacatgtct
      361 gccctcctgc tgcccttcca tcatggcctg ttgcctggct tccagtgcct cacgctcttc
      421 ccggctgaca cgggcaatcc atgcatccag cacctggtat gcgccgaaca gaacaaggat
      481 ggccgcaatc aactccgcca gaaaaaaagc catccagggt ttgctgtaaa caagacgggc
      541 gacgatatga aaccagacac ccagggaaaa tacccacatt cccgtaatat caagccgggg
      601 taaagagaat ctgcgtttac ttatcatgtt aaactccggt tattatttac atgagagtat
      661 ttatgtctgt aatattcgcg tttgtgtgat attaatcaga tcaacctcat tattttgtat
      721 ggtttttatt accgttttat atgacttagc ctgtcataag cccccttata aaaaaccaca
      781 tcactccgga ccatactgat gacaacataa tacagataaa tattcttctg aacccgtcga
      841 agctcgtaac gttcggtaat gagccaataa ccattggaac aaccattgct gcgccctgat
      901 tcataattgc accacacaga aaccagagac tggcggtaag cacatataaa cacgcgatat
      961 taataacaag gaaaaccagt gagctcagga gaaacatttc cctgtcatta cgctcagaaa
     1021 agaaataacc ggcaataatg acaaatatta acaaaacaac acctgtaaac attttcaaat
     1081 aatcttcact cataaatcaa atccgcgatt aaaaattggg tttaaaatct tcagaaacgt
     1141 tcaggaactg ttttgccagg tcgtcctgcg aaataaagcc gtatgataac gggcgaacac
     1201 tgccctgttt cgggtcaacc agcatcatgg ccgggaaata tttcacgccg aggcgctgcg
     1261 cctgcccctg gtcagtccgg gaatccggca acagcggatt aatcacacca tccacggaaa
     1321 cgggaataac actcagacca taggtatccc tgaagccatt aatgacctgc gccagctgcc
     1381 cgtcgatggg gtcctgcccc cggtaaaaga acatgatgcc gtagtgttca gccagtttcg
     1441 caatggcctg tcgctgctgc acctggtctg ccgccagctg gttccggacc gtgccgttgt
     1501 aatggctgta ctgcaggtta tagtccagtt cgggatgcgc cagcatggct tttttggcgc
     1561 tcatggtgaa aagcccggcc tgctgagtcc agtaattctg cagccggaaa tatttcacaa
     1621 aattatccac gccgggatac agaatagctt cgtacagcgc ccgctttgtt gccgtctgca
     1681 gtgcggccag tttctgcata atgtccggct cctgacgggg ggctgcaggt actggtttat
     1741 tttctttttc cttcggattt attttctcgt tataccactg ccagcctgca tcttttcctg
     1801 acgccggaaa aataaagcag cagagtaaca gtggcagtaa tgctttattc atcttcattc
     1861 ctttcatcac tttttctttc atcaggtttc tgcaggtcag tcagggcaga aaaacaaaaa
     1921 tacagaataa acaggagcac accggagtaa aaaataacga aagcagcagc aagtatctca
     1981 cctgcagtgg tttcttttac cgtatcccac gaccagataa ttaccgggga aatgacaatc
     2041 gctgtaatca aaagccggat aagaaaatca ataaaacggt tcgacgtgaa tatgactttc
     2101 atcattttcc ccggttattt tccctacctt actgtcctgc ctgtttcagc tggtcagcaa
     2161 tctgctcttt cactttctgc gtcagaacac cactgtccgg gattttctgg ttattcatca
     2221 ggtcttcgta gaagttagtg aaatccagtc tgtcgaactg aattttctgc agctcatcaa
     2281 ccgtaatccc ccgacagtca ggatgcttcg cactgccaaa actgatacgc agctgtccgt
     2341 tgcgcccctg ctgctggact atctgcgcca gcttcgaatc aaactgacag taactgcgtt
     2401 ttttctccag gcagacaccc agcactttct tcgaacagaa ctctccgaca ctgacggtca
     2461 gtttgtttga ttttgcttta gccagggctt tttcatcact gctgcatttg gccagcccga
     2521 tatcctgtcc ccagccgctg tccttacagc agttgctgta tccggctgca gccttcttac
     2581 aaaatttcgc ctgaccggtg aaagcacgga catccacccc gttcagcgcc gcaacatcct
     2641 tacctgctgc ggccagtgcc gccagctgag aaacggcttc ggcaaaatca ttactcttcc
     2701 cgctctgagc cttatcgcat tcgccatcga gacagaagac gtctccccca cagaccatca
     2761 ctttcccgga tgtttttgac tcacaggagt acgtggcata ttcatgcaga caggcacctt
     2821 cttcggagta aaaggcgcac tggtgtgaca ccagggtaca ggcaggattg tcggtgtagg
     2881 tctgacaggt tcccttgtct gctgactgtg tgacatacgt gtcccggtat gcccagcagc
     2941 tctggtacat gctgtatgac tgaccgtctt tcaccagcgt cctgttcccg ccagcctccg
     3001 tacattctgt ttttgtcagt ttccccagct ccttgctgaa cgggcagctt tcagaccaga
     3061 caacatacgg ggtgtacgag gcttttttta ccctgacaac cagacgcagc ttcattgccg
     3121 tatgacgtct gaattttgta ttcccgaccc ggacggggtg agatgacgta aaagcaacac
     3181 cggcagtcag tgttttctgt actggtgtaa atgaggcatc tgctgagtat ttggcattcc
     3241 agctcagtgg ggttcccagt actgtaattg tcataggtac actcttctgc aggtaagtac
     3301 gactccagct gtaatgcgca tcgacaattt caccggtaac gggagaggta atggagacaa
     3361 catactgacc attcacttca cgtgcaggta gctgactcat ttcatactcc agcgtccggg
     3421 tttcccatgt ggtactcccc tgcagctcca tacgggcagt tcgtgtgcag tactgctcca
     3481 cctgtaaatc ccgctcacag gtgtaattcg tgtattcact ccggctaatc tcctgcgcac
     3541 tgcactgctg cccggtgttt cccacaatgc tgtcagcccg gttcaccaca tccctgccgg
     3601 tctggataaa cggtgcatcc ggtgaaagaa tgtctttcgg cttgttcatg aatgactctg
     3661 tgatggtttt tccggtttca ccggtcgccc attccgtggt gccgtcattt ttcaggccgc
     3721 catccccgcc ggcagtcacg ccaccgtagt attttgtctc gtccggattc gcgttatagc
     3781 cggggatact ctcctgtggc ttaaagccct gaatactgct gcttccctgt cctttgatct
     3841 ggtgagcaaa atcagagccg gcccggtaat cactgttgct gtcagccagt gccatgccgg
     3901 caaccagagc cagtatcaga ggtaaaatac gtttcatttc ccggaatctc ctttccctgc
     3961 cagtaaatca tgagccacct gccggcagtc acccgtggcg gccactttct ccagcgcctg
     4021 gccgacccgc aggtttcccc ggatgatgtc gtatccctga ctgcagaaca ccaccagtgc
     4081 cggtacactg cgaatgccgt actgtgaaaa cagcgtcggg tcgatctgaa caccatcagt
     4141 cgcgccgtct ttcaccaggg acagcacggc ttcagcggtg gtcttcaggt cattgttcac
     4201 catgccccgc agtgtggcgg gtataccgta gtgccgggtt tcgcccagca tccgtttcag
     4261 cccctcttcg ggaatggaaa acgacacaaa atacagggcc ccctgccggg gtttcccgtc
     4321 ctgcgaagcc tgctgtttac gaaccagctc atccaggaaa tggttatctg aacgctgaag
     4381 ggggttctcc agcacctgtt tctccgccca ggccttcagc tgatggtcag gtttttcacg
     4441 cagttgtctg cttaaatttt cctgctgctt caggaactgg cggttttcag gagtgttcac
     4501 attgtctgag gcatgaacag tgatatgcat tcctgcagtc agggcaaaaa gcgcaaatgt
     4561 tttccagttc atgatatatc ctgatggcat tgttacaatt taattacccg tagggttttc
     4621 tctcaatttt tctgccagtt ttaaaaaagc atcaaactct ttctgacctg cgcatatttt
     4681 actggctgta atcgacacgg ttattcctga tgtgttattg gcagagtctt taatatcatt
     4741 attatggacg gagacattat cgggactgaa aatataaact ttttctctgg gataatccct
     4801 gtccacatcc tcccgccact gaatctgttc tttatcaaaa cggtgataca ttattgagga
     4861 aaaccggcac aaccagtcgt caatacatga cagcttatcc agtaattgtt gcgaatgacg
     4921 gacctcacag gcaagctgcc gacactccat aatgcaccgt gacacaccac catttgcatt
     4981 cacaagcgct ttcatctcca gcagaacacc catatcaagt attctggagc catattttat
     5041 attattactc attacaccct cctctgtttt tgtttacaca tttacctggc ataaatatta
     5101 tctgtattac gatgccttga tggcgtataa acattgcaac tcctttctct gtcagaattg
     5161 aaggacaatg caataacacc atcaaacaac agttcgataa actcagactc acatgagtct
     5221 gcaaccacaa ccaggcgttt atgatatttt ctggcctgtt taattaacct gataactttt
     5281 gatgtcattt tcttttcaca cagcctgact ccatcaagga ttaacgtctt ttcaggtgta
     5341 ttcagaaaat caaaattatc aatattttca tcacaaagcc ggttgtaccc cgcagagtta
     5401 agagttttaa agttgtaata ccgggaatca ctatcactga ttcttatttc agtcaggaga
     5461 gttgacttgc cggtatcttc ttccccctta atcagaatgc ggtctttaaa gttcagcatt
     5521 aattctgttg cctgtttata cataaaaaat ccttcagaat tagtagattt acaggaagac
     5581 acagttacgt ttacgccaca tcaggtagcc gaagtttttc tttgtattgg gcggattttt
     5641 cccggtttcc cagcgggtca cactgcgccc gaacggatgg cactgtccgc tgtccgggta
     5701 catattcacc atctggtaac gccagcgttc tttgggcagg attggggacg gatattcatt
     5761 acagacggcg ttatttttcc cgatggtttc c
//
LOCUS       DAEVZA010000215         5709 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEVZA010000215 DAEVZA010000000
VERSION     DAEVZA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.215"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(27..995)
                     /locus_tag="LAF92_004809"
     CDS             complement(27..995)
                     /locus_tag="LAF92_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HBI9793698.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVP
                     LRCPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRI
                     WRKLHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETA
                     MYRMKQLLGDSLTLRDYDGQVAEAMAMVRALNRMTKAGMPESVRIA"
     gene            1029..1340
                     /locus_tag="LAF92_004810"
                     /pseudo
     CDS             1029..1340
                     /locus_tag="LAF92_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
     gene            1407..1562
                     /locus_tag="LAF92_004811"
     CDS             1407..1562
                     /locus_tag="LAF92_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HBI9793699.1"
                     /translation="MSFKANYESLASIMPRNEQEADAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            1504..2166
                     /locus_tag="LAF92_004812"
     CDS             1504..2166
                     /locus_tag="LAF92_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HBI9793700.1"
                     /translation="MLAWRLNLQLRMNIPPRATRTVFCVGSGPSLTREDCAAIEKTGC
                     SIIAVNNSWQMFDDIYALYAGDLSWWKQYGSTIPGGRFRKVTANLAAAKSFSLEYRRY
                     CGPAEGVNSGAQAISLAAESGAEVVVLVGYDCSLQNGLHWHGAHPQALRNPTQVSISK
                     WQQQFLDTRKKHADLHILNASRSSAIQCFPRINLEAVIALLSSAVAQAPQTLLRRAEC
                     RL"
     gene            2064..2690
                     /locus_tag="LAF92_004813"
     CDS             2064..2690
                     /locus_tag="LAF92_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HBI9793701.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGTEP
                     KNTNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSAREQIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            2687..3364
                     /locus_tag="LAF92_004814"
     CDS             2687..3364
                     /locus_tag="LAF92_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBI9793702.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDADQEILARALVELVRRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQHYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            3361..4047
                     /locus_tag="LAF92_004815"
     CDS             3361..4047
                     /locus_tag="LAF92_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793703.1"
                     /translation="MKIALVLRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILENPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSAFWQDPEGWIARCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLISSFF"
     gene            4365..5627
                     /locus_tag="LAF92_004816"
     CDS             4365..5627
                     /locus_tag="LAF92_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793704.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLQIEH
                     LKRQQQDAIDDATHDANQRADNAERISIELETKLNEMTAMVELRNLQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESR
                     RPELVRRVEWAQEVMLADAELPFEPEFIDDLATKGLHTLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
BASE COUNT         1466 a         1303 c         1493 g         1447 t
ORIGIN      
        1 gaacgaccct gtagctggct ggattttcag gcaatacgca cgctttctgg catcccagcc
       61 tttgtcatcc tgttcaacgc acgcaccatg gccatagctt ccgctacctg accatcgtag
      121 tcacgcagcg tcagtgaatc tcccaacaac tgcttcattc tgtacattgc cgtttccgct
      181 atcgagcgac ggttatattc cgttgtccat ttccaccgtg cattgcttcc gctcagccgc
      241 tgattagcaa cggcacggtt gcggtctgcg tactcaccgg gccagtaacc tgctcctttt
      301 cggggaggaa taagcgcgct gatttttttg cggcgcagtt catcgtgaca gagccgggta
      361 tcgtaagccc cgtctgccgc ggctgccctg atttttctgt gagtctgccg gataaggccc
      421 gggaaggctt ctgagtccgt gacgttattc agcgacaggt ctgcacagac aacttcatgt
      481 gtgttgctgt caacagcaag atgcaacttt cgccagatac gacggcgctc tttgccgtgc
      541 tttctgactt tccattcgcc ttcaccaaag accttcagcc cggtggaatc aatcaccagg
      601 tgtgcgattt caccccgggt ggacgttttg aaactgacat taaccgactt tgcccgctta
      661 ctgacactgg tgtaatccgg gcagcgcaac ggaacgttca tcagggcaaa aatggaatca
      721 ataaaaccct gcgcagcccg cagggtcagc cggaatacgc gtttaatcac cagaacggtg
      781 gtgatggcga gatcagaata gcgctggggc cttcctcgtg atgaaggcgt tgccgactca
      841 taccaggcct gaatcgcctc atcatccagc cagaaagtga gggagccacg gttgatgaga
      901 gctttgttgt aggtggacca gttggtgatt ctgaactttt gctttgccac ggaatggtct
      961 gtgttgtcgg gaggatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa
     1021 caaagccagg gaaacgttca aaagcaaccg ccgggaaaat gaaggctgaa gggcagaaaa
     1081 aaggttatcc ggacatgagt ctcgataaag catgcggtat ttatcacggc atgcgaattg
     1141 agcttaaaga accaaatggt aaagccccga cgaaagagca gatcgcctgg atgcgcaggc
     1201 ttagagagga aggttactac gtcgttcttg cgtatggtgc agaacaagcg ataaccgcca
     1261 tcctggaata cataagcctt aaaaagggtg aggctattga gcatgtattg aacggcgaca
     1321 agtggttgta tgctgcttaa aataataaat taattagtgc atatgcgctc tttgatatag
     1381 cgcacattaa catcgggaga ataatcgtgt cattcaaggc taattatgaa tcgctggcat
     1441 cgatcatgcc gcgtaatgaa caggaagcag atgctgtagt ggacccggta atcgctgaaa
     1501 tgaatgctcg cctggaggct gaatttgcag ctgagaatga acataccacc cagggcgact
     1561 aggactgttt tttgtgtcgg tagcggtccg tcactcactc gtgaggactg tgctgctata
     1621 gaaaaaactg gctgttcaat catcgcggtt aacaattcct ggcagatgtt cgatgacatt
     1681 tatgccttat acgccggtga tttgtcatgg tggaagcaat acggatccac cataccggga
     1741 gggagattcc gcaaagtgac agccaacctg gcggcggcga aatcattttc gttggagtac
     1801 aggcgatatt gtggaccggc ggaaggggta aatagcggcg cgcaggctat cagtctggct
     1861 gctgaatcag gggctgaagt agtggtatta gtcggctatg actgttctct gcaaaacggc
     1921 cttcattggc atggcgcgca ccctcaagcc ctacggaatc caacgcaggt gtctatttca
     1981 aaatggcaac agcagttcct ggatacccgc aaaaaacacg cagatttaca tattttgaat
     2041 gcaagtagga gcagtgcaat tcaatgtttc ccaagaataa atttagaggc agtgatcgcg
     2101 ttattatcgt cggcagtggc ccaagcgccg caaactttgt tgcgccgcgc ggagtgccga
     2161 ttatagcggt caatggggcc atcgactggc ttaaccgcgc ttcttatttt ttcacccttg
     2221 atccatcgcc agacaatatg cggcgcgttg gtcgtggccg ccgtcgccgt ggtgtttgtt
     2281 attgcatggc actacccgat gttaaagaac gtgaagtcag agatggcgtt ctgtgcttcc
     2341 gtcgtgtggc tgagcgcggc acggagccaa aaaatacgaa ttctcccgag tggtgggcgt
     2401 ggcgctggtc cgcacatttc ggcctttgcg aagatgagaa tgaaattgcc agcggcaata
     2461 gtgcatatgg tgctctgaac ctggctttcc atatcggatt caaacatgtc gccctggtgg
     2521 gcgttgacgc tacacaagaa ccacgcgttc actccggcgg cacgccaaaa aatctaagtc
     2581 acctgccttt gttattccag tctgcgcgtg aacagattga cgttgtttca tgcgggaaaa
     2641 tgggaggtat tccgcagatg actcttaaag aatggctgaa gaatacatga tggcacccac
     2701 aatttatcac cgtatcgacg gtaccaaata caggaatgtc tgggttgttg gtgatctgca
     2761 tggttgctac accagactga tgtccgaact ccatcgtgtg gattttgacc cggcgcagga
     2821 tttactgata tcggtcggcg accttatcga tcgcggtact gaaaatgtcg aatgtctgga
     2881 actattgcag atgccctggt tcagggcagt gatggggaac catgagcggc tgatgattga
     2941 tgcgttaagt ccagatggca acgtgaataa ctggctaatg aatggcggtc aatggttctt
     3001 catgctggac gctgatcagg aaatattagc cagggcgctg gtggagttgg taagacgact
     3061 gccctatatc attgagttga acaccgggca agaaactatc gttatagccc atgccgacta
     3121 tccggataat gaatatcaat tcggtaagga ggtgccgctt ttcaacgttg tctgggcgcg
     3181 cgagcgtatc agtgattcga tggatgatat tggtggcgaa atttctggcg cagatcgttt
     3241 tatctttggt cacactccgg tgaaaagccc gaagacattc tggaatcagc attatatcga
     3301 cacaggtgcc gtattttgcg gaaacctgac attgatgaaa gtgaaaggtg atggtgcagc
     3361 atgaagattg ctttagttct tcgctctggt ggtgactata acgcttccga tgtgcagtgg
     3421 ctggttaatc aactgccaaa aggctatgaa attatttgcc tgacagacct gaagcgttta
     3481 catgtacctg gcgtcaaagt tgtcccattg atcaaccagt ggcaaaagtg ccgtggctgg
     3541 tgggcgaaaa tcgagttgtt ccgaccggat ataaccgatg atctgttcta tctggatttg
     3601 gacacggtta ttgccggtga tatacgccca atcctagaga atccaccaac cagcttcacc
     3661 atgcttaggg atttttacca tccacaatat cgtggcagcg gtgccctgtg gataccaaat
     3721 agtgtaaaag cgcatatctg gagtgcattc tggcaagatc cggaaggttg gattgctcgt
     3781 tgtgttacta ctgaatgctg gggtgatcag gggtttttgc ggaaggttat gggtgatgat
     3841 acaccagcat ttcaggatct gtatccggga tggtttgtaa gttacaaggc cgatgttgtg
     3901 gaacctggtt cgaaatatgc gagcgcgcgt tactccaggg ggaatggggc attaccaaaa
     3961 gactgccgaa taatcttttt ccacggcaaa ccgcgacctc gcgaagtgtc agaggattgg
     4021 cttcccctta tcagctcatt tttttgagcg agaatcagaa taatattgct ctaataattc
     4081 attattttta aaacgtgatg tacactcatc atgtttttta tcagagcaat ctacaaggtg
     4141 cactatgtgg ccattccgac ggaaatatca ctactggctg atcgcctttg ttacgccgac
     4201 cggcggtatc aggcatgtca tcaccaggta tcgcaacaag agactcacct tagccagaat
     4261 tttacaggct gccataggtg agggactgga tacaaattgc gtagtccttc caccttcata
     4321 cttaggaaaa atgaccgaag cacaagctaa tacggaactt tgaaatgagc acttcagcac
     4381 aaaaccaatc aatcgaaaat gtatctatcc ctgatgtcct gaatgccggt attccggcca
     4441 ttatccagaa catccgggcc gcgcaacgcc gcgttagttg tgatgacctc acagcacgtt
     4501 tttttgataa tgcggttcag tcagcggaga tgcttcacgc acagcttatt gatgtttata
     4561 acgcagaagc tgatagccat aactccctgg tagatgcagc tgaaaatatg cagttggatc
     4621 tcggtctgaa gggtaaagaa attgaagagc ttcagctgca aattgaacat ttgaaacgcc
     4681 agcaacagga cgcgatcgac gatgcgacgc atgacgccaa ccagcgtgct gataatgccg
     4741 aacgtataag cattgagctg gaaacaaaac tcaatgaaat gaccgcgatg gttgaactgc
     4801 ggaacttaca gatttcaacg ctaaaatctc aatataaaga gatcatgaaa cttgatcctt
     4861 ttaaccttga gaaacgctat aacaaagcta aaagcgagcg acaggaactg cgtaagcagg
     4921 tcgccgacct taaccaacag ctcaaaaaaa ctattaaaga tgcaagcgag gcgcgcgtgg
     4981 catttgctaa taaaaaagca gaggttaccg cgctggttaa tgagaatgcc aaatttgcga
     5041 cgctcaagaa ggaaatgtat ggcattactg agcgccgttt ccctgcaagc aaacttcatc
     5101 cgacgttagg gcaaatctcc ttcttcccgc gcctcctggc ttatgggatc tcatcgccta
     5161 aagagttcaa taacgagcgt ccttatatcg tttctaagct ggactttgct tatcagttct
     5221 gctgcgacat gggctatgcc attgatatcc gaatcaacga atggttgatg ccaaacttcc
     5281 agccgttggc aattttccgc gagtttcagc cggaaggttg ggtagagttc ttccatgaat
     5341 tgatctgtaa agagatggaa agccgccgcc cggaattggt ccgtcgagtt gagtgggcgc
     5401 aagaggttat gttggcagat gcagagctgc cgttcgaacc ggaattcatt gatgatctgg
     5461 caactaaagg gctgcatacc ctgtttgatg tggttacccg ccgtcatgag cagttggttg
     5521 tcgaattggg tttagaggaa actgcggcaa gaagacttct cgatgtttgc tatgcacgta
     5581 gcgatgcatg ggaaaaagag aacggcggca ctatttacgt tcgctgatag ttacagtgtc
     5641 acttttaatg atggtggagt gctcccacca gcattttttt cgtccaatga ggagggcatt
     5701 tgagtattt
//
LOCUS       DAEVZA010000216         5706 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEVZA010000216 DAEVZA010000000
VERSION     DAEVZA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.216"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..917)
                     /gene="emrA"
                     /locus_tag="LAF92_004817"
     CDS             complement(<1..917)
                     /gene="emrA"
                     /locus_tag="LAF92_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBI9793705.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPA"
     gene            complement(1044..1574)
                     /gene="emrR"
                     /locus_tag="LAF92_004818"
     CDS             complement(1044..1574)
                     /gene="emrR"
                     /locus_tag="LAF92_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBI9793706.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(1665..2000)
                     /gene="ygaH"
                     /locus_tag="LAF92_004819"
     CDS             complement(1665..2000)
                     /gene="ygaH"
                     /locus_tag="LAF92_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBI9793707.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(1990..2727)
                     /gene="ygaZ"
                     /locus_tag="LAF92_004820"
     CDS             complement(1990..2727)
                     /gene="ygaZ"
                     /locus_tag="LAF92_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBI9793708.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(2851..4035)
                     /locus_tag="LAF92_004821"
     CDS             complement(2851..4035)
                     /locus_tag="LAF92_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9793709.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(4227..5219)
                     /gene="proX"
                     /locus_tag="LAF92_004822"
     CDS             complement(4227..5219)
                     /gene="proX"
                     /locus_tag="LAF92_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBI9793710.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(5277..>5706)
                     /locus_tag="LAF92_004823"
     CDS             complement(5277..>5706)
                     /locus_tag="LAF92_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBI9793711.1"
                     /translation="VTIIFALPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQ
                     LPLAMPTIMAGVNQTLMLALSMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIV
                     ILAIILDRLTQAVGRDSRSRGNRRWYTTGPVGLLTRPFIK"
BASE COUNT         1413 a         1475 c         1518 g         1300 t
ORIGIN      
        1 tgcgccggaa gcagtgagaa tgcgctacct gtgcccatat ccagaccaac cactttaccg
       61 gtgtatttca catcatcgcc gtaaatatcg gtggtgatag tgaccggctg accgatacgc
      121 atattggcaa tctgcgtctc tttaaagttg gcatccaccc acatattggt ggctggaacg
      181 accgccatca gcggcgtcgt tgggctaatt tgcgccccag gctgtaccgc gcggcgggag
      241 acataaccgg tcatcggact gacaatacgg gtacgctcca gcgccagcca ggcgttacgt
      301 acttcggtgg cagcctgttg cacggcaggc tgatcttcca gtttagtccc cagaatcatc
      361 gcctgattgg cattgtattg ttgaatagcg acgtccagtt gcgcctgggc actggtgacg
      421 gcgtcgcggg cgtgttgcag ctcttcgcga ccaatcaggt tggcattgcc cagcggcaca
      481 cggcggttgt agtcgctttg tgctttcgcg agggcgattt tttgcacctc aatattcgcc
      541 tgcaactgtt tgctgttaat catcagctgg tgggtttggc gaacgctgga agccagtgca
      601 gttttggctt tttcaaacgc ctggcgagca tctgtcgggt cgagagtgac cagaacatcg
      661 ccttctttta caaaatcggt gttatcggcc cagactttcg tcacgctgcc agacacctga
      721 gacataattt gaatctgatt ccctgccacg tatgcgtcat cggtttcttc gaaatgacgc
      781 agtaccaaaa accaataaat ccctatcgct acggcaataa ttataaagag caaggtgaga
      841 aggaggagca gacgcttacg tttgccgctc ttctttaccg gttgctgcgg ggtttgagtc
      901 tccgcatttg cgctcatatt gttctccacg atcttcttaa ctcatcggct gagccgacct
      961 gcttgccaaa aaggccagca tgttgcgatg ctggccggtc attttttctt atataaatct
     1021 ggatttttga gcgagatgac gcgttagctc atcgcttcga gaaccacacc gtcttgttcc
     1081 atctggtcga gacgggagag caatttgcgg gtgatttgct cgagctgatc tttttctgtt
     1141 gtgctgagcg cggaccagag ttgatgcagg cagttatgct gcggtggtaa aacctcgcgc
     1201 aaaaactcgt gacctttttc cgttaattgc agatgcaggc agcggcgatc gttatcgctt
     1261 tcacgacgtt cgatccaacc gcgtttttcc agttcatcgg caatacgcgt cgcgttggta
     1321 cgggatgatc caagagcaca acttaattca gaaggctgaa tactgtggtt ttcctgagac
     1381 tccagcgtaa tcaacgccat aaacaacgtc tcgttaatcc cctgagcctt cagcatttta
     1441 ttgcggttct ccagcagctt gctttgcatg tgcatgcaaa gacgagtcag aaggatctcc
     1501 tgataaggaa aatcttcgtg gcggctggcg cgaaatttta gcatttgttc aatgggcgta
     1561 aacgaactat ccatttgggt atgacctcat taatttcagc cgatatagta acgacagtga
     1621 caaataaagt aaatgtattg ttttagaaaa atgattcttg tgggttatat aatcgccatc
     1681 actttccagg cgagcccata ggccagcgca ctaagcagtg ttgggatgat aatgctgcgt
     1741 gttttataga aactggcacc cagtaccgcg aagccgacca gcgtgggcac gaaacggcgt
     1801 gtatcgtgca tcacttctgg tgcggtagag acaaccagca gagcgcatat cgaggcgatg
     1861 ccaatggtgt cgagcaaaat acctaccgcg ccacgtttgg ttgggcgggc attacccaca
     1921 cgcaggcgca gcggcaaata gcggaagcaa taattcgcca cgccaaccag taacccaagc
     1981 agcagaacct catagctcat cgggcgctcc ttgccagaat gcctggatta acgcagtgag
     2041 gcagccacag acaatgcctg ccagaatggc gacgggaata gaaaatagcg ttacgcctgc
     2101 aagggcacca actaacgctg cggtaacgca aagagattgt ttgcgctgga aagaggcgag
     2161 caggaaactc ataaagagtg ccggaagcat aaaacctaat gcagcttcaa cggcgggata
     2221 accttgcagc aagccgctgc cggagaatgc ccctattacc gtaccaaata cccacgatga
     2281 ccatgaactg aaggcaatgc cgatcatcca gttctcgctc cagcggcgat tattgcgtac
     2341 cagttttgcg gttgcggcgg caaaaacctc atccgtcagg ccaaatgccc acagggcggt
     2401 tttcgatttt tgcagacgct gaataatacg gctacgcagt gacgggccat acaacacatg
     2461 gcgaacatcc attgccatga cggtcagtgc agcaacccac aaactactcc cggctgccag
     2521 catcgcggta atgacgaact ggctcgcgcc tgcataaatg atgcaggaga aaaaaacgct
     2581 ttcgagagga gagaatccca gacgggtcgc attcagaccg aacgcaaagg ccaccggaat
     2641 ataactaata acaatcggta aactgtcttt gcatccttcc atgaaggtcg ccgaaccagg
     2701 agcaggctgt ggagtagggc tttccataga gtgtacgctt aacgattgtt aatattataa
     2761 atagactgaa tgaatatctt aaccttatca gactgatggg cttcttaaca cccttataag
     2821 tgtaaagcca cgaaaacggt tgctgattgc ttaatttgcg gcttcctgac gatgaaaacc
     2881 tcgccaccag accagtaagt taaccagggc aatcgtcgcg ccagccagac aaacgccagc
     2941 ccaaccgcca tgttgccagg ctgaggctga aattagcgaa ccggcggcac cgccaataaa
     3001 gtagctggtc atgtaacctg cggtcaggcg attacgcgca tcagggtgta ttcgataaat
     3061 taccgtctgg ttagtgatat gcacgccctg tacggtgaga tccagcacca ggatgccgat
     3121 aatcagcgcc agtacggaag tgtgaccaaa ccagatcgcc agccatgaaa gtaatagcag
     3181 cagcagaccg aaagttgtgg tgtggtgcga tttaccctta tcggcaaaac cgcccgccgg
     3241 acgagcgccc aacgctccgg cagctcccgc aagtccaaac agaccaatga caccatcgct
     3301 gtagttaaaa ggtggagcgg caagcaaaaa ggccattgag gtccagagaa tgctgaagtt
     3361 ggcaaaggtc aggcagccca gcaatgcgcg ggtgcgcagg atcttatcgc tgataaacat
     3421 gctgaaaacg gaacctaaca actgtgggta gttgaggtgg gtttctgatt tcatttgtgg
     3481 cagaccacgc cataatgcca gcgccatcag tgccattaac accgaggcaa cccaaaagac
     3541 ggtgcgccag ccgccgagat tcgccagcaa tccggcaact gtccgtgcca gcaagatccc
     3601 caacagcaga ccgctcataa tagtgccaac cactttgccg cgtttgtccg gtgaagccag
     3661 cgtcgctgcc agcggaacca gaatttgtgc cacgacagag aataaaccgg ttaatgccgt
     3721 accgaggatc atcatcgcca gcgactgact gctggcagtg attaacatgc cgcctgccgc
     3781 cagtaaggtc atcgagacaa tcaggcggcg gcgttcaaac atatcaccga ggggaacaag
     3841 aaacagcaga cctgctgcat agcccaattg cgcggcggta acaataaagc ctgccgaact
     3901 ggcggaaagg gaaaagttac gcgcgatggt gtcgagcaat ggctgagcgt aatagttact
     3961 ggcgaccgcc agaccggtgg cgatagacat cagcacgatc agcgccgggc taagctcatg
     4021 attaggttta gtcattattt ttgttcatca ggtgatgatt aagctggaaa tcaataatag
     4081 cggatgttga tgaatatggg atgaaagata gcttgttgaa atgtgcgatg tggtttgtag
     4141 gcatgataag acgcgtcagc gtcgcatcag gcattgtgta cagagcggcg gatgaggcaa
     4201 aaacgcctta tccgcctgaa taaaaattac ttctgcgctg ccagcgcctc attcacccag
     4261 ccatcgaact gctgctggtg ggctttgatc cagccatcaa cgtggccctg aatatcgcct
     4321 tctgaggctt tgccgtcatg cataatggcg ttctgggcgt taatatcggc cactggcaac
     4381 tgcataatgg caaacagttt ggctgccgcc ggatttttct cggtccaggc tttgttggca
     4441 acgatatgca tggtgctgac cgggaagccg taattcgcgc cattcggcag tttggtatcg
     4501 gcatttttat cgcccggcag tgcggagaac ggcacctgca accagaccac atctttccct
     4561 ggcttcagct cattactcac ccagtacggc gtccaggtgt aataaaacac cggtttgccc
     4621 tctttgtagc gactgatggt gtcggccatc atcgctgcgt agttcccctg attatgcgtc
     4681 acggtgttgg tcagttcata cgcggcaagc tggtggttga tggcaccttc gcagccccag
     4741 ccagggttac aaccggttaa atccgcttta ccgtcgccgt tggtatcgaa cagtttggcg
     4801 atcttcggat ctttcagttg tgcgatgttg gtgattttat actggtcggc ggttttctta
     4861 tcgatcaggt aaccctgtgc cgcgccgtta acaaataccc cttcacgata aaatttctta
     4921 tcgccaccgg cagcttcgta catgttgtca tgcagcggcg tccagttcac ggcggtgaag
     4981 gttgcatcgc cggaagcaag cgaggtgtag ccaacgttgt aatctacttc gctgggttta
     5041 ttgacggtat aacctaattt ctccagcgca cgactgacca gcagtgtctg gaaggtttct
     5101 tcggtgatgg tgctttgaac tggattaact gtaatgcctt tgcccggcag gtcggcagca
     5161 aaagtttgtg tagagataag cgtggcaaac gctgtcgcaa aaagtacgct atgtcgcatt
     5221 gttattcctt tttttggcag ggtgataatg cccggcgtca ccgggcaagt gcagagttac
     5281 ttaatgaatg ggcgggtcag cagaccaaca gggccagtgg tgtaccagcg acggttgccg
     5341 cgactgcgtg agtcgcgccc aacggcctgc gtcaggcgat cgaggataat ggcgaggatc
     5401 acaatcccga cgccgccaac ggtggcaagc cccatatcca gacgaccgat accgcgaagt
     5461 accatctgac ccagcccgcc gacggcaatc atcgaggcaa tgaccaccat agaaagggcc
     5521 agcatcagcg tctggttaac gcccgccata atggtcggca tcgccagcgg taactgaact
     5581 ttgaacagca tctggcgcgg gctggcaccg aatgagcgcg aggcttcaat cagatccgcc
     5641 ggaacctggt taatccccag aatggtcaga cggataatcg gcggcagagc aaagatgatc
     5701 gtcacc
//
LOCUS       DAEVZA010000217         5684 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEVZA010000217 DAEVZA010000000
VERSION     DAEVZA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.217"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..262
                     /locus_tag="LAF92_004824"
     CDS             <1..262
                     /locus_tag="LAF92_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HBI9793712.1"
                     /translation="FRGRYQDPVAPERDQLISTLNGLVAPFGVLPETAVLNVPLSTRQ
                     HPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQSLR"
     gene            275..1927
                     /locus_tag="LAF92_004825"
     CDS             275..1927
                     /locus_tag="LAF92_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBI9793713.1"
                     /translation="MRNTLKQAVVLWGMVLLLVLWSVFISPSGVLRWAGAAAIVLAVA
                     ALLIYRRRQAWTEMTGDAGLSSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTVMPGMYRDAAVLTGRLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPEKAHSPWLICTGGEIQVATSAETASPAQWLTQTSTQE
                     RSQPLCYLLKAESLMQWLNLYVLAALNGPEAKCPPLAMAVGLHPSLPAVDNNLWQLWI
                     TARTGLTTDIADTGTDATLPFPDALLRRLPRQSGFTPLRRASVTMLGITTVAGIAALC
                     LSATANHQLLRHIGDDLHQFYAVPAEEFITKARRLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGEQIRQPVLRAIRDWRPPEQKMEVTASLQAQTVRLDSMSLFDVGQARLKDGST
                     KVLVDTLVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            1985..2476
                     /locus_tag="LAF92_004826"
     CDS             1985..2476
                     /locus_tag="LAF92_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBI9793714.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKEPSKEKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            2667..5312
                     /gene="tssH"
                     /locus_tag="LAF92_004827"
     CDS             2667..5312
                     /gene="tssH"
                     /locus_tag="LAF92_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HBI9793715.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLTALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQVTEPLTSAQPEAATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLQARLTALDIEREAIEQDSVFYPEASPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     AQQIMTLRQEGTDSTELQQQLRAHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVVR
                     RLRQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHH
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            5324..>5684
                     /locus_tag="LAF92_004828"
     CDS             5324..>5684
                     /locus_tag="LAF92_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI9793716.1"
                     /translation="MGIQNFDHSHHKLKIRGQQSPVDVLTFEGREQLSTPFRYDIQFT
                     STDKAITPELVLMQDGAFSLTAPPVQGMPVQAPLRTLYGVITGFKHLSSSQDEARYEV
                     RLEPRMALLARSRQNAIY"
BASE COUNT         1222 a         1660 c         1736 g         1066 t
ORIGIN      
        1 atttcgcggg cgttatcagg atccggtggc cccggagcgc gaccagctta tttcgacgct
       61 caacgggctg gtcgcgccat ttggcgtact gccggaaacg gcggtactga acgtgccgtt
      121 atccacccgt cagcaccctt tgctgcattc gccgtttttc tggctggtaa cgctgacgct
      181 gctactggcc ggtgtctggt gggggctgca ccactggctg aacgtgctgg tggatgaatt
      241 gctgccgcaa agcctgcggt aaagggagtc gtgagtgagg aacacgctga aacaggccgt
      301 cgtgctgtgg ggaatggtgt tactgctggt gctgtggtcg gtgtttatca gtccgtctgg
      361 cgtactgaga tgggccgggg cggcggctat cgttctggcg gttgccgcgt tgctgattta
      421 ccggcgcagg caggcgtgga cggagatgac cggcgatgcc gggttgtcat cgctgccgcc
      481 ggaaacgtac cgacaaccgg tagtgctggt ctgtggcgat atgtcggcgc atctgttcac
      541 tgacagcccg gtccggcagg tttcagaagg gctgtatctg catgttcccg atgaagaaca
      601 gcttgtggcg caggtggagc gattactgac ccttcgcccg gcgtgggcat cgcagcttgc
      661 cgtggcgtat accgtcatgc ccggcatgta ccgggatgcg gcggttctga ccggacgact
      721 gcgacggttc gcccacagta tggcgacagt gcgtcgccgg gcgggcgtaa acgtcccctg
      781 gcttctctgg agcgggctgt ccggctcgcc cttgccggag aaggcacatt caccgtggct
      841 tatctgtacc ggcggcgaaa ttcaggtagc gacgtctgct gaaaccgcct cccccgcgca
      901 gtggctcacg cagaccagca cacaggagcg cagccagcca ctctgttacc tgctgaaagc
      961 cgaaagcctg atgcagtggc tgaatcttta tgtgctggcg gcactgaacg gcccggaggc
     1021 gaaatgcccg ccactggcga tggccgtggg gctacacccc tcgttgcctg cagtggataa
     1081 caacctgtgg cagctgtgga tcaccgccag aaccggcctg acgacggata tcgcggacac
     1141 cggcacagac gccacgctgc cgtttccgga tgccctgtta cggcggttgc cgcgtcagtc
     1201 gggctttacc ccgctgcgac gggccagtgt aaccatgctg ggcatcacca ccgtggcggg
     1261 tatcgccgcg ctgtgtctgt cagccacggc gaatcaccag ttattacggc atatcggtga
     1321 cgatctgcac cagttttatg ccgttccggc ggaggaattt atcaccaaag cccgtcgtct
     1381 gtcggtgctg aaagacgatg cggtcatgct cgatgggtat taccgggaag gagaaccgct
     1441 gcgtctcggt ctggggttat accccggcga acaaatccgc cagccggtat tacgcgccat
     1501 tcgtgactgg cgtccgcctg agcaaaaaat ggaagtgaca gcctcgcttc aggcgcagac
     1561 cgtgcgtctt gacagtatgt cgctgtttga cgtcggacag gcccgcctga aagacggctc
     1621 gacaaaagtg ctggtggaca cactggtgaa catccgggca aaaccgggct ggcttatcct
     1681 cgtggccgga tataccgacg ccaccggcga cgaaaaaagc aatcagcagt tatcactgcg
     1741 acgtgccgaa gcggtgcgta actggatgct gcagaccagc gacatcccgg ccacctgttt
     1801 tgccgtacag ggactgggtg agagccagcc tgcggcgacc aacgatacgc cacagggccg
     1861 ggcagtcaac cggcgcgtcg aaatcagtct tgttccgcgt tctgacgcct gtcaggacgt
     1921 gaaataaaac ataccgccgg aagaaggcgg tgcttcaatc acactaacaa ggagagtaat
     1981 tctcatggct attcctgctt atctctggct gaaagatgac ggcggcgcgg atatcaaagg
     2041 ttccgtggac gttcaggggc gcgaaggcag catcgaagtg gtggcgctgg atcacgatgt
     2101 gtacatcccg accgacaaca acaccggcaa actgaccggt acccgtactc acaagccctt
     2161 tacgtttacc aaagaaatcg atgcgtccag cccgtatctc tacaaagcag tcaccaccgg
     2221 ccagaccctg aaaacggcgg aatttaagtt ttaccgcatc aacgatgccg gtcaggaagt
     2281 ggagtacttc aacatcacgc tggataacgt caagctggtc agagtcgctc cgctgatgca
     2341 cgacatcaag gagccttcca aagagaagca taaccacctg gaacgcattg agttccgcta
     2401 cgagaaaatc acctggactt acaaagacgg caacatcatt cattccgact cgtggaatga
     2461 acgtccttcc gcctgataac cgtggcagac aggaaactgt ctgcctttct gttgtgtgct
     2521 ttgcccagtg ccgcaggcac cggtactgag caaagcatac ccacaatctg gtgatacctg
     2581 atgggcctga gtgaggtaac ggctatgggc ggtagcgtac ctgaacgcag atcgcaggaa
     2641 tacagaacac gataaggaaa ccttctatga ccggaaatca ccccgccgcg ctgctgcgtc
     2701 gccttaaccc gtactgtgca cgggcgctgg acgctgccgc ctcactgtgt cagacccgcg
     2761 cccatgcgga gataaccatt gaacactggc tgctgaaact gctggagcag ggagaaggcg
     2821 atatcacggt gattgcccgc cgctatgaat gggatatcga cacgctctgg cagtctctgc
     2881 tggcacatct ggacacctta ccccgctcgg tccgcgaacg tccgcagctt tctgaacccc
     2941 tgacggcgct tatcaggcag gcgtggctga ttgcctcgct ggaaggcgac gacccgcaaa
     3001 tccgcagtca gcacctgctg atggcgctga cagaaaaacc gatgctgccc gcctgtaatg
     3061 acctgtgggt attgctgagc ctgagccgtg tgcagcttga gcggctgcgt cccctgctgg
     3121 atgcgcagtc ggatgaatgt ccggcacgtc agccacaggt caccgaaccg ctgacatccg
     3181 cgcaaccaga ggcggcaacg acggacgcac cggcaaaaac gctgacgggg aaacaggatg
     3241 acgccctgct ggcggtgctt aaccgcttta ccgaagacgt gacggaaaaa gcccgcagcg
     3301 ggcgaatcga cccggtattc gggcgcgaca cggaaattcg ccagatggtc gatatcctct
     3361 cacgtcgccg caaaaacaac ccgattctgg tgggagaacc gggggtgggc aaaaccgcgc
     3421 tggtggaagg gctggcgctg cgtatcgccg aaggcaacgt gccggacagc ctgaaaacgg
     3481 tgcatatccg cacactggac ctcggtctgt tacaggctgg cgcgggtgtt aaaggtgaat
     3541 ttgaacagcg gctgaaaaat gtcatcgatg cagtgcagaa atcaccggag ccggtactgc
     3601 tgtttattga tgaagcccat accattatcg gtgcgggtaa tcaggcaggc ggcgcggatg
     3661 cggcgaacct gctgaaaccg gcgctggcaa ggggcgaact gcgcaccatc gccgccacaa
     3721 cgtggagcga atacaaacag tattttgagc gcgacgccgc gctggaacgc cgcttccaga
     3781 tggtgaaagt cgacgagccg gatgatgaca ccgcctgcct gatgctgcgg ggactcaagg
     3841 cccgctatgc acagcaccac ggcgtacata tgctggacag cgccattcag accgccgtgc
     3901 gcctgtcgcg ccgctatctg accggacgcc agttgccgga caaggcggtt gatttgctgg
     3961 ataccgccgg ggcaagagtc cgcatgagcc tcgacaccct gccggaaccg ttgacgcagc
     4021 ttcaggcgcg tctgaccgcg ctggatattg agcgggaagc aattgagcag gacagcgtgt
     4081 tttatcccga agccagcccg gagcggctgg cggaactgac cgatttgcgt gatgagctgc
     4141 aggcagaagc cgggcatctg gaagcgcagt atcagcagga aaaggcactg gcgcagcaga
     4201 ttatgacgtt gcgtcaggaa gggacagaca gcactgaact gcaacagcaa ctgcgagcgc
     4261 atcagggctt tgctccgttg ctggcgctgg atgtggacgc ccgcgccgtc gccacggtgg
     4321 tggcggactg gaccggcatc ccgctctctt cgctgctcaa ggacgagcag agcgacctgc
     4381 tcagtatgga acaaagcctt gaaaaccgcg ttgtcgggca aagcccggcg ctctgcgcca
     4441 tcgcacagcg gctgcgggcg gctaaaaccg gcctcacgcc ggagaatggc ccgcaggggg
     4501 tattcctgct gaccggcccc agcggcaccg gtaaaaccga aactgcgctc acactggccg
     4561 acaccctgtt tggcggtgaa aaatccctta tcaccatcaa cctctccgaa tatcaggagc
     4621 cgcataccgt ctcccagctt aaggggtctc ctccgggcta cgttggctac ggtcagggcg
     4681 gcgtacttac cgaagccgtt cgcaaacgcc cgtacagcgt ggtgctgctc gacgaagtgg
     4741 aaaaggcgca ccgcgacgta atgaacctgt tctatcaggt gtttgaccgg ggttttatgc
     4801 gcgacggcga agggcgggaa attgacttcc gcaacaccgt gattctgatg acggctaatc
     4861 tgggcagcga ccacatcatg cagctgctgg aggaaaaacc ggacgccaca gacgcagacc
     4921 tgcatgaact gctgtatccc ctgctgcgcg agcacttcca gcctgcactg atggcgcgtt
     4981 ttcagacggt gatttaccgc ccgctgggac aggaagcgat gcgcgccatt gtggaaatga
     5041 aactggcgca ggtggttcgt cgtcttcgtc agcactatgg gctggaaacg gaaatcaacg
     5101 acagcctgta cgacgccctg accgccgcct gcctgctgcc ggacaccggt gcgcgtaata
     5161 tcgacagcct gctgaaccag caaatcctgc cggtcttaag ccagcagttg ctggcacagc
     5221 aggctgcgca ccataagcca gcccaactac ggcttggctg ggatgaggaa gacgggattg
     5281 tactggagtt cgctacggaa gagatgcaat aacggaggct gtcatgggta ttcagaattt
     5341 cgaccactcg caccacaagc tcaaaattcg cggccagcaa agtccggtgg atgtgctgac
     5401 ctttgaaggc cgcgagcaac tgagtacccc gttccgctac gatatccagt ttaccagtac
     5461 cgataaagcc atcacgccgg agttggtgct gatgcaggac ggcgcattca gcctgacggc
     5521 accgccggta cagggaatgc cggtgcaggc ccccctgcgc accctgtacg gggtcattac
     5581 cggttttaaa catctctcat cgtcacagga cgaagcccgt tacgaagtac ggctggagcc
     5641 gcgtatggcg ctgctggccc gcagccgcca gaatgccatc tacc
//
LOCUS       DAEVZA010000218         5659 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEVZA010000218 DAEVZA010000000
VERSION     DAEVZA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.218"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..625)
                     /locus_tag="LAF92_004829"
     CDS             complement(<1..625)
                     /locus_tag="LAF92_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBI9793717.1"
                     /translation="MAFDDLRSFLQELDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGND"
     gene            complement(625..1218)
                     /locus_tag="LAF92_004830"
     CDS             complement(625..1218)
                     /locus_tag="LAF92_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBI9793718.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            1365..1772
                     /locus_tag="LAF92_004831"
     CDS             1365..1772
                     /locus_tag="LAF92_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBI9793719.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDSADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(1893..2885)
                     /gene="rpoS"
                     /locus_tag="LAF92_004832"
     CDS             complement(1893..2885)
                     /gene="rpoS"
                     /locus_tag="LAF92_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBI9793720.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(2948..4087)
                     /gene="nlpD"
                     /locus_tag="LAF92_004833"
     CDS             complement(2948..4087)
                     /gene="nlpD"
                     /locus_tag="LAF92_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBI9793721.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(4227..4853)
                     /gene="pcm"
                     /locus_tag="LAF92_004834"
     CDS             complement(4227..4853)
                     /gene="pcm"
                     /locus_tag="LAF92_004834"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBI9793722.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(4847..5608)
                     /gene="surE"
                     /locus_tag="LAF92_004835"
     CDS             complement(4847..5608)
                     /gene="surE"
                     /locus_tag="LAF92_004835"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBI9793723.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGSRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPDTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
BASE COUNT         1306 a         1566 c         1436 g         1351 t
ORIGIN      
        1 gatcgttacc gagcgtgatc gcaatcggca gatcttcacc gcgctcttct gctttatgca
       61 gatgcagggc gatatcgtgc atcggcaccg gttgcaggcc gagcttacgc ttgcccttca
      121 cttccatgcg gtagatgccg acgttctgct tgccgaagtt atccgggtcg agcggatcgc
      181 gggaaaccac gcacgctttg tcgagataga aaccgccatc gccatcgttt aaacgaaaca
      241 gcggcaggat atcgaacagg ttgatctcgt cgccatcaac ggtgttctgc gcccaggctg
      301 gattggcgcg gcgctccggg gcaatcggga agttatccca gcggcggata aactcatcaa
      361 tctgcttttt aaccggggca tttggcggca ggccgaggga aatcgcgtgg ttctgccagg
      421 aaccgatggt gttcatcgcc acgcgggcat cggtaaagcc gcgaatatta tcaaaccaca
      481 gcgcgggcgc gccgtcgccg atacgcccgg tggcgttagc tgctgctgcc agatccggct
      541 cggcgttcac ttcttcgctg attttcagta actggccgtg gtcatcaagc tcctgtaaaa
      601 agctgcgtaa atcatcaaat gccattattc attctcctga gaaaaattcc gggcctgcgg
      661 caatccttgc cagcgcctgg cgtggggatg ttcgaggcca aattgatcca gcacgcgggc
      721 taccacatgg tggacaatgt catctaccgt ttcgggatgg ttataaaagg caggcatcgg
      781 cggcaccatc gccacgccca tgcgtgaaag tgcgagcata ttttcgagat ggatggtgct
      841 aagcggcatt tcacgcggca ccagcaccag tttgcggcct tctttgagca cgacgtccgc
      901 cgcgcgccct accaggccat cggcgtaacc agcgcggata ccggcgagcg ttttcatact
      961 gcacggaata acgatcatgc cgtcggtacg aaaagaaccg gatgagatga tcgccgcctg
     1021 atccgccggg ttatggctga agtctgcgag ggcagcaaca tcgcgagcgc tgtaaggcgt
     1081 ttccagttca atggtggttt tcgcccactt cgacatcacc agatgagtct cgacattcgg
     1141 catctcccgc agcgcttgca gtaatgccac accaagaggc gcaccggtag cccctgtcat
     1201 cccgacgatc agtttcattg cttattctct taattgttcg tatacgaacg ttattattaa
     1261 catactcctt catatccaca gcggcaagga ttgaaaagta acaacatctt cttcgtcact
     1321 aaaaaaccgc tatgatagcg acagagaatt agcccggagt ttacatggcg ttacgaaata
     1381 aagcgttcca tcagttacga cagctttttc agcagcacac ggctcgctgg cagcacgagt
     1441 tacctgacct gaccaaacca cagtacgcgg tgatgcgcgc cattgctgat aaacctggta
     1501 tcgaacaggt ggcactgata gaagcagcag tcagcaccaa agcaacgcta gcagaaatgt
     1561 tggcaagaat ggaaaatcgt ggcttagtca gacgtgaaca tgactccgct gacaagcggc
     1621 gacgctttgt ctggctgact gccgaaggag aaaaggtact tgctgcggcg atacctattg
     1681 gtgacagcgt ggatgaggaa tttttggggc gtttgagtgg agaagagcag gagctgttta
     1741 tgcagctggt gcgcaagatg atgaacgcat agggggcaaa tttccggatg cgacgctatc
     1801 gcgtcttatc cagactacaa atacaccgac tcaccaaccg aaagaaaaaa ggccagcctc
     1861 gcttgagact ggcctttctg acagatgctt acttactcgc ggaacagcgc ttcgatattc
     1921 agcccctgcg tttgtaggat ttcgcgcaaa cggcgcaggc cttcaacctg aatctggcga
     1981 acacgttcac gggtgaggcc aatttcacga cctacatctt ccagtgttgc cgcttcgtac
     2041 cccagcaaac cgaatcgacg tgccagcact tcacgctgtt tggcgttcag ctcgaacagc
     2101 catttgacga tgctctgctt catatcatca tcttgcgtgg tatcttccgg accgttctct
     2161 ttttcatcgg ccaggatgtc cagcaacgct ttttcggaat caccacccag cggggtgtct
     2221 accgaggtaa tgcgctcgtt aagacgaagc atacggctga cgtcatcaac tggcttatcc
     2281 agttgctctg cgatctcttc cgcacttggt tcatggtcca gcttatggga caactcacgt
     2341 gcggttcgca ggtaaacgtt cagctccttt acgatgtgaa tcggcaaacg aatagtacgg
     2401 gtttggttca taatcgcccg ttcaatcgtc tggcgaatcc accaggttgc gtatgtcgag
     2461 aagcggaaac cacgttccgg gtcaaacttc tctaccgcgc ggatcagccc caggttgccc
     2521 tcttcgataa ggtccagcaa cgccagacca cgattgccat aacggcgggc aatttttacc
     2581 accagacgca agttactctc gatcatccgg cggcgagagg cgacatctcc acgcagtgcg
     2641 cgacgcgcaa aataaacttc ttcttcggcc gttaacagtg gtgaataacc aatctcacca
     2701 aggtaaagct gagtcgcgtc caacacacgc tgtgtggctc cctgcgataa cagttcctct
     2761 tcggccaaat cgttatcact gggttcctct tctactaagg ccttttcgtc aaaaacctca
     2821 actccgttct catcaaattc cgcatcttca tttaaatcat gaactttcag cgtattctga
     2881 ctcataaggt ggctcctacc cgtgatccct tgacggaaca ttcaagcaaa agcctggttc
     2941 cgccgattta tcgctgcggc aaataacgca gcgggtttac ggatttcccc ttgtaacgaa
     3001 tttcaaaatg caagcgtgtt gaactggttc cggtgctacc catggttgct attttttgcc
     3061 ccgccttcac ttcttgttgt tcccggacca gcattgtgtc gttatgggcg taggcactca
     3121 ggtaatcatc attatgtttg atgataatca gattaccgta gccgcgcagc gcgttaccgg
     3181 cataaacaac gcggccatct gcggtcgcga taattgcctg tcctttgctg cctgcgatat
     3241 caatcccctt gttgcccccc tcagaagcgc caaaggtttc gatcactttg ccctcagtcg
     3301 gccagcgcca ggtggagata ggcgtactgg ttgatgtact gctgacagtc ggctcggttg
     3361 tgcttgctgt tggtaccgtt acaggcgctg tgaccgtggt cgcagttggc ttgttgttcg
     3421 gcaacatttt gttagcactc tgttcacccg aagactcaga atacgtaatt gtcggttgcg
     3481 acgcaacagc aacggtggaa ttttgtgcag gcttgatcac aactccttgc tctgctgcat
     3541 cggcctgggt aatggcattt ccgccagtga ttggcgtacc ggaagcatta cccacctgta
     3601 aggtctgacc gacgttcagc gcgtatggtg cctgaatatt gttgcgctga gcaaggtcac
     3661 ggaaatcgtt gccagtaatc caggcgatat agaaaagtgt gtcgcctttt ttcacggtat
     3721 aggtactgcc gctataactg cctttcggaa tgttcccata ctgacggtta tagacgatgc
     3781 gtccgttttc catctgtacc ggctgctgag ctactggctg tactggctgg atttgcggtt
     3841 gttgagtagc ctgaatttgt ggctgctgta ccggctgaat ttgcggttgc tgcgctgtag
     3901 acgtcgttcc cattttcggc ggcggcgtaa tcaacatacc agaattggta tttgcaggcg
     3961 cattgccatt aacggagctg accggtgccg gtggatttga agtgtcagaa cagcctgcca
     4021 gccatagcga aaccagtgac aaagccgcaa tgcggcgaac ggtgaatttt gggcttcccg
     4081 cgctcattta tcccccagga aaaattggtt aataaccagt gacataatta ccgtgcaagg
     4141 caccatactg aacactggaa aagatgttca cgatacgctg acctgcggca aaataaccag
     4201 gaaaaatcca ggtatttcct cacgttttaa gccagctcac ccttcactaa agggacaaag
     4261 cgcacggcct ccacggtatc gataataaat tcgcctcccc gacgacgcac ccgtttcaaa
     4321 tactggtgct cctcccctac gggtaagacg agaatcccgc cttcgtccag ctgcgtcatt
     4381 agcgcagttg gaatttccgg cggtgccgcc gtaacaatga tagcgtcaaa cggcgcacgt
     4441 gcctgccaac cttgccatcc atcgccatga cgggttgaaa cattatgtaa atcaagattt
     4501 ttcaggcggc gacgcgcctg ccactgcaag cctttaatcc gttcaaccga gcaaacatgc
     4561 tggacaagat gcgccaggat tgccgtttga tatcccgaac cagtgccaat ttccagcacc
     4621 cgcgactgcg gcgtcagctc gagtaattcg gtcattcgcg ccaccatata cggctgcgaa
     4681 attgtctgcc cctgacctat cggcaaggcg atattgtccc aggctttttg ttcaaacgct
     4741 tcatcaacga atttttcacg cggcacggcg gcaagtgcat tcagcacctg ctcatcctga
     4801 ataccttgcg cacgtaattg atccagaagt gcttgtacgc gtctgcttac cattgcgtgc
     4861 caactcccac gctgtttaac cagtctgaaa ccacatcttg cgcgctatgc gcagttaaat
     4921 ccacatgcag cggcgtgatg gagacatagc cttcatctac cgctgcgaag tcggtatccg
     4981 gcccggcgtc acatttcccc cccggaggac caatccagta cagtgtatta ccacgaggat
     5041 cttgctgcgg gatcacctga tctgccggat gacggctccc acagcgagtc acgcggattc
     5101 ctttgatttg gtccagcggc aaatccggta catttatatt aagaatacgc cctgttcgca
     5161 gcggttcttt gcacagtgca cgcagaatag aacaggtaac tgccgcagcg gtgtcgtaat
     5221 gtttatgccc gtctagcgag acggcaagcg ccggaaaacc taaatggcga ccttccatcg
     5281 cggcggctac cgtaccggaa taaataacat catcccccag attcggcccg gcgttaatcc
     5341 cggacacaac aatgtccggg cgcggacgca tcagagcatt cacgccaaga tagacgcaat
     5401 cggtcggggt tcccatttgc acagcaatat caccattttc aaaggtaaac gtgcgcaggg
     5461 aggattccag tgtcagagaa tttgaagcgc cgctgcggtt acgatcgggg gcgaccacct
     5521 gaacgtcagc aaactcacgc aaggctttcg ccagcgtttg tataccgggt gcatgtaccc
     5581 cgtcatcatt actcagcaat atgcgcataa tcacctgttg tgttgataag ttccctgaca
     5641 acgctggttg caaaactac
//
LOCUS       DAEVZA010000219         5539 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEVZA010000219 DAEVZA010000000
VERSION     DAEVZA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.219"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..329)
                     /locus_tag="LAF92_004836"
     CDS             complement(<1..329)
                     /locus_tag="LAF92_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9793724.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAV"
     gene            complement(556..1770)
                     /locus_tag="LAF92_004837"
     CDS             complement(556..1770)
                     /locus_tag="LAF92_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9793725.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTTIAAIGFLLALFIGLRSRKKTR
                     HQSLKENVAPGG"
     gene            complement(1870..2226)
                     /locus_tag="LAF92_004838"
     CDS             complement(1870..2226)
                     /locus_tag="LAF92_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBI9793726.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(2362..2469)
                     /gene="rprA"
                     /locus_tag="LAF92_004839"
     ncRNA           complement(2362..2469)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="LAF92_004839"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(2655..3767)
                     /gene="ydiK"
                     /locus_tag="LAF92_004840"
     CDS             complement(2655..3767)
                     /gene="ydiK"
                     /locus_tag="LAF92_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBI9793727.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            4156..>5539
                     /locus_tag="LAF92_004841"
     CDS             4156..>5539
                     /locus_tag="LAF92_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBI9793728.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVL"
BASE COUNT         1576 a         1320 c         1353 g         1290 t
ORIGIN      
        1 cacacagcca gaccgtaggc gagagttaaa ttcgggcaag cgggaatacc aaagaagaat
       61 agcagataca ttattactgc cattaatatc accgcccgac gaccaaactt atcggagatc
      121 acaccgaaga ataaaatact gatcaatcgc cccaaaccga taccggaaat taaataggca
      181 atgcccgcgt tgtcagtgga aaacttttcc gccagagatg acatgttttg ggcaagcgta
      241 ataacactaa tgccgtgcag gaagtagctg aagtaaatac aaagaacagc caggataaat
      301 ggcgtgctga aagccttatt ttgagacatt ttcacagctc cttgctgaaa gataatttcg
      361 ttaccgtacg tattgttctc cgacggttta cataattact atactgaaga tgattttccc
      421 tgggtacatg gcataaagtt tatgtttcca ggaatattgc atgtcatagt ttgtttttga
      481 tcgcgatttt ccttagttag cgcagaaaga gttatttatc cgtaagatct tgcatgacct
      541 gaaaagaata ttgcattacc cacccggagc gacattttcc tttagcgact gatgccgcgt
      601 ttttttgcgg ctgcgtaagc cgataaacag tgccagtaaa aaaccgatgg cagcgatggt
      661 ggtatcgaac cacataatat cggcaatact tctttgggac agatgagccg taatcaacgg
      721 aatagtaaag gtcgcaatac tgcccgcact gtaatagatc cctgtagctt tacctttagc
      781 gtaagggaaa cgttcagcca ttaacgtcag gccaatttgc acaacacctc cagcagaggt
      841 aaaaccaatg acaaaagcaa atattatcac cacataaaat gtgggatgca ggcagacggt
      901 aaacagagca ataaatgaga taaaggtgta cagcatcagt aatgttgttg ggcgaacggt
      961 attacgaatg agtggagcgg taataaatac acaaagcagc gaacccacgg tgtagatact
     1021 gagtagttta atcgacatag tgtatgacat gcctgcaaca aattgtccgt actgtgccag
     1081 ccactggcta accagataaa acgttgccat tgagatatag ccatataagg tataactggc
     1141 taaatcgata attgaacagc gatgttccgt agagctggtg gttttcttta tgacaggtaa
     1201 gcgatgaccc ggatggggtg ggaacgtaca acggtataaa aacagagcgt taataaacat
     1261 aatgcctgca gcaatcataa aggaccaacc gaaccacagt tcagcccaca ctaacaggct
     1321 aatgattagc ggtaataaaa attgtccgct ggaaacaaat gctttaatta aaatattggc
     1381 tgtcccaggt gagcgtggaa aagcttccat caaactggga taagtgcctg catcgagaaa
     1441 actgtttgcc attcctgcca gaaagccaaa aacataagcg ataatgatgt tattggtgtg
     1501 caggatgcca aaaaagaagg ccatatagca gcacatcccg agcatgataa aagggcggcg
     1561 accaaagcga tcggataata atcctgcaaa aagcaagaca cttaatcgac caatgcccag
     1621 cgatgagata actatcgaga cacccgcggc attagtctgc caaagtgtct ccagcgaggc
     1681 catattcagg ctcatcaaaa tgacgcccat accatgcacc aggtaattaa aatacaaccc
     1741 aagtgcggta gggtaatagg gatttttcat aggttctact tagcaaaggt gctttaaaaa
     1801 gtgtagcgat gttgtgtcag tatccgttca ttattgttgt aacaacgaca attccttttt
     1861 gtggagaaat taacaaagcc gctccagatc gtgtgcatat aattcagcgg ctaccgattg
     1921 atagatcttc cagtcaataa aattgccatc agggtagact tgttgaaacg cggttaagtc
     1981 ggggaaaaaa cgcatggtgt tgttgccgat gcggttattt attttctcta tgatggcatg
     2041 aagatgtttt tttcgttgct gacgcatagc caacagttgc tcgactacac gatcaggaat
     2101 ggcgcatttg ccattttccc actgacgcca gctttcactg tcaccggtct gggcaatcca
     2161 ggtcgcgcat tcatcaatag tcatggcaaa aatatggcga agtgcctgga gttcataagc
     2221 gttcatcgtg taatggctgc atgaaataaa caataaaata taaataacat tatcagcctg
     2281 ctgacggctt gaagagagtc acagtatctt gtgcaacatt attggaaaga gtgaggggcg
     2341 aggtagcgaa gcggaaaaat gttaaaaaaa agcccatcgt gggagatggg caaagactac
     2401 acacagcaat tcgttgtttc actcagggga tttccatgct tataaatcaa tatgttgatt
     2461 tataaccgta agctaatagt aggcatacgg actttttgcg tcgatcagat tcgtctcatt
     2521 agtttaaaaa ctgtaaagaa tttgcgacag gtactaccag ctgaatggcg gtaagcagga
     2581 aaatatgggg aagagcaagc aaatatcaat tagcgggggt taacaatcag gcggcagagc
     2641 cgcctgatga gaaattactt attcggtttc tcgatttcgc caagttcttc gagaatttct
     2701 tccggttggt ccgtcggcgg cgggacttct tccacccacg cggcaaacag acgccaggaa
     2761 acggctaaca gaaccggacc aataaacaga ccgatcatcc cgaaagcaat caaaccacca
     2821 ataacaccag agaggatcag gatcagcggt aaatcggcac ccatgcgaat taacattggg
     2881 cggatgacgt tatccagcgt gccaaccaca ccgctccaca ctaacaatac cgttccccag
     2941 gtggtatcgc cagtccagta gagccagata atcgccggaa tcagtaccgg caacgggcca
     3001 agctggacaa ggcaggagag gatcattaac accgttagca acgttgcata aggtacgccg
     3061 gatacggcga ggccgatacc gccaagcact gcctgtacta acgccgtcac caccacaccc
     3121 agcgccaccg cgcggatagc ctgtgccgcc agcagtacgg cggcatctcc gcgaacgcct
     3181 gccagacggg ttgcaaaatg gcgaatgcct tgtgccacct gttcaccgcg ccagtacagc
     3241 agggcactga agagaagcat cagcgcacaa tgcaccataa agcgcccgat atgcgccgcc
     3301 tgcccaacga accaggtggt ggtggtgcca atataagggc ggactttcgc catgatcgcc
     3361 gtccccccca tatccagcaa gttgtgccag cctgcataca gcttcgcgcc aatcaccgga
     3421 atggtattaa gccacgccag atcgggtaac gtcatgtcgc cgctggaaat ggctttaatt
     3481 agcgggccgc tgccgtcgac gatactgtta accagcaaag caatagggat gataaacacc
     3541 atcactaata acagcgtcat caccagaacg gcgagagagc ggcggccaaa catgatcttt
     3601 tgcaaacgta acaataccgg ccaggtggcg ataaccaccg taccggccca tgcaaagccg
     3661 agaataaagg gttgaacaat ccacagacat gccacaatca tgatggctaa aaacagcacc
     3721 gaaagcagaa tttgtgcgac atccctgggc tgacgaacat ttaccataac tacttttcac
     3781 cttttttgtg cgtcaaatcg tcgacgcgac gtgaacacgc gaaactcacc tgcataatga
     3841 tgagcaattt cagcggtttt taacaggccg attctgccct taattctgat gggcgtgtta
     3901 gaaaaaaatg tggtacaaca actcaggcaa cacgcaaacg atttactcgt cgtatttcaa
     3961 gttgcatgtg ctgcggctac gttcgttcac cccagtcact tactattgta agctcctgga
     4021 gttcactcgc ttgccgcctt cctgcaactc gaaatccata gagtaaataa acatcacctt
     4081 tcagtgcgct attgcagccg cctgaaagaa tagtgtatcg acctacatca cagaccgcag
     4141 gaaagggtca atataatgat tccacagatt tcccaggcac ccggcgtcgt tcaactggtg
     4201 cttaattttt tgcaagagct ggagcaacaa ggttttaccg gcgatacggc gacaagttat
     4261 gccgatcgtc tgacaatgtc gaccgacaac agtatttacc aacttctccc cgatgcggtg
     4321 gtatttccgc gttcaaccgc agatgtggcg ctgatcgccc gtcttgccgc gcaggaacgc
     4381 tattcatcgc tgatctttac cccccgtggc ggcggcaccg gcactaacgg tcaggcgctc
     4441 aaccagggga ttattgttga tatgtcccgc catatgaacc gcatcatcga aattaaccct
     4501 gaagagggct gggtgcgcgt tgaggccggg gtgataaaag atcaactcaa tcagtacctg
     4561 aaaccgttcg gctacttttt tgcaccagaa ctttcgacca gcaaccgggc aacgctcggc
     4621 gggatgatca ataccgatgc ttctggtcag ggatcgctgg tctatggcaa aacgtcagat
     4681 cacgtacttg gcgtacgcgc ggtgttgttg gggggcgata ttctcgatac gcaaccttta
     4741 cccgtcgaat tggcggaaac gctgggtaaa tccaatacca caatcgggcg aatttataac
     4801 acggtttatc aacgttgccg tcagcaacgc cagttaatta tcgacaactt ccccaaactt
     4861 aaccgctttc ttaccggtta cgatctgcgt catgtcttta acgatgagat gaccgagttc
     4921 gacctgacgc gcattctgac gggttcagaa gggacgctgg cctttattac cgaagcgcgg
     4981 ctggatatta cgcccttgcc taaagtgcgc cgtctggtga acgtcaaata tgactctttt
     5041 gactccgcgc tgcgtaacgc gccgtttatg gttgaggcgc gggcgctttc ggtagagacg
     5101 gtggactcaa aagtgctgaa tctggcgcgg gaagatattg tctggcattc cgtcagcgag
     5161 ttgattaccg atgtgcctga caaagagatg ctcgggctga acattgtgga gtttgctggt
     5221 gatgatgagg cgctgattga cgagcgagtt aatgctctct gtgtgcggct tgatgagctg
     5281 attgccagcc aacaggcagg tgtgattggc tggcaggtgt gccgcgagct ggcgggtgtt
     5341 gaacgtatct atgcgatgcg taaaaaagcc gttggtctgc ttggcaatgc caaaggtgcc
     5401 gctaagccaa ttccgtttgc tgaggatacc tgcgtaccgc cggaacatct ggccgattat
     5461 attgctgaat ttcgcgcgct gctcgacagc cacggcttaa gctacggtat gttcggtcac
     5521 gtcgacgcag gtgtcttgc
//
LOCUS       DAEVZA010000220         5469 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEVZA010000220 DAEVZA010000000
VERSION     DAEVZA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.220"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..69
                     /locus_tag="LAF92_004842"
     CDS             <1..69
                     /locus_tag="LAF92_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBI9793729.1"
                     /translation="ILKTTDYLRIAQPELLNFRRTA"
     gene            complement(70..174)
                     /locus_tag="LAF92_004843"
     CDS             complement(70..174)
                     /locus_tag="LAF92_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793730.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            344..790
                     /locus_tag="LAF92_004844"
     CDS             344..790
                     /locus_tag="LAF92_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBI9793731.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(747..1568)
                     /gene="nimR"
                     /locus_tag="LAF92_004845"
     CDS             complement(747..1568)
                     /gene="nimR"
                     /locus_tag="LAF92_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HBI9793732.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            1665..2846
                     /gene="nimT"
                     /locus_tag="LAF92_004846"
     CDS             1665..2846
                     /gene="nimT"
                     /locus_tag="LAF92_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBI9793733.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            2901..3248
                     /locus_tag="LAF92_004847"
     CDS             2901..3248
                     /locus_tag="LAF92_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBI9793734.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3270..3524)
                     /gene="yoaF"
                     /locus_tag="LAF92_004848"
     CDS             complement(3270..3524)
                     /gene="yoaF"
                     /locus_tag="LAF92_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBI9793735.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            3707..4732
                     /gene="dgcP"
                     /locus_tag="LAF92_004849"
     CDS             3707..4732
                     /gene="dgcP"
                     /locus_tag="LAF92_004849"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBI9793736.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            4765..4863
                     /locus_tag="LAF92_004850"
     CDS             4765..4863
                     /locus_tag="LAF92_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBI9793737.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            4866..4940
                     /gene="yoaJ"
                     /locus_tag="LAF92_004851"
     CDS             4866..4940
                     /gene="yoaJ"
                     /locus_tag="LAF92_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBI9793738.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(4999..5247)
                     /locus_tag="LAF92_004852"
     CDS             complement(4999..5247)
                     /locus_tag="LAF92_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBI9793739.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
BASE COUNT         1307 a         1357 c         1325 g         1480 t
ORIGIN      
        1 attttgaaaa ccacggatta tttgcgcatt gcgcaaccag aactgctcaa tttccgccgc
       61 actgcgtaac tagcctgtcc gttcaataag cagaacggac aaaaccagca caacagcaat
      121 ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac atttgatcgt tcatagcatg
      181 cccccgggag atagctgttt tcacgttatt gttagcgtgc acaaatggca gtttgatgac
      241 agttcgcctg attttttatg caaaaaacgt aaagattttc tacttccttc ctgcagcaag
      301 cgtaaagtaa gcaggcttat tattttttgg caaggaaacc acgatgtttg atgtcacttt
      361 gctgatcctg ctcgggttag ctgcgctggg ctttatcagt cataacacca ctgtcgccgt
      421 ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta agcacctttt tcccgtggat
      481 tgaaaaacag gggctaagta tcgggataat cattctgacg attggcgtta tggcccccat
      541 cgccagcggg acgctaccgc cctcaacctt gattcactcg tttctgaact ggaaatcgct
      601 ggtggcaatt gcagtaggcg ttattgtctc ctggctgggt gggcgcggcg tgaccttaat
      661 gggcagccag ccgcaactgg tcgccgggct gctggtaggc actgttttag gcgtggcgct
      721 gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt ctggtttcgc tgattgtggg
      781 gaaacagtag ttaatcccgc aatatatcgg cctggcgttt gccccaggcc tttcttaaac
      841 atagtaataa aggccgttgt tgaatcatac cccagcgtat gagcaacttt ctgtacagta
      901 tcgcctttca ctaatccctg aagtgccata atcagctgta actgctgacg ccactggcga
      961 aaactcaacc ccgtctcttt tactatcagt cgcgcaagat tgcgttcact catcgcaaaa
     1021 aagccagccc attgccctaa tgacccccac tcgacgggcc ctttcgccat catctccacc
     1081 atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca gctgttgttg tggctgctga
     1141 gggagttcat caaacaggac ctgaattaat ctgcgggtca tcggctcagc acgctgagtc
     1201 gtcgtcctgt ttgccagggt taaaatcaat tcccggcata agggcgatat tttcaatgtg
     1261 caacatgttg tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg
     1321 ttagctgtca cttgattgct atgttccacc ccgcccggta tccaaaccgc atattgcggc
     1381 ggcaccatcc acaaagcatt ttccaccgta caggtaattg caccatgtaa tgctagtatc
     1441 agctgtcctt tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata
     1501 caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat agccattgag attcaacctg
     1561 tgcatcattt tgtccgaatt tagcgataat ttgtcatttt agcttgattc aacataacaa
     1621 taaaaacggt aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt
     1681 cattaagcgg caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat
     1741 tacgcgtcac ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc
     1801 tgacgacagc gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct
     1861 cacctttggc tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt
     1921 tacttttgat ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg
     1981 gcggtacagc ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa
     2041 ttaaacgcga tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg
     2101 gagctgcagc ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct
     2161 ggcaaggcgc gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc
     2221 cacagtggcg aagccaacaa catgcaaatt tgagtacctc gcgcgcctta catactcggg
     2281 gtatctggcg ttcgccgctt gcctggcagg tcacattgtt tcttgggatc aactcactgg
     2341 tctattacgt gattattggc tggcttccgg cgatcctcat cagtcgcggc tatagcgaag
     2401 cacaggcggg ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga
     2461 tccctctttt cttacatcat gtgaaagatc agcgtggtat tgcagcgttc gttgccttga
     2521 tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc gcacgcgatc acctggactc
     2581 tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc
     2641 gggcaagttc tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt
     2701 tgctggcagc ctgtgggccg ccgctgatgg gtaaaataca cgatgctaac ggtgactggt
     2761 ccgtaccact gatgggtgtt gccatacttt cactactgat ggcgattttc ggactttgcg
     2821 ccgggagaga caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcatgcg
     2881 tgaaagggag aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac
     2941 agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg
     3001 atttagccct tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct
     3061 ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca atatcttgca gaactggcgc
     3121 aacacgagca agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc
     3181 tctacgcagc aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta
     3241 gcttgtgatt ttagtacagc atccggcggt tatttttcac cagccggatg gtcccgtcgc
     3301 cacaatgccc attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct
     3361 tgcgctgtct gcactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga
     3421 tttgccatac caatctgcgg cggtttaggt gcttctggct gagaaggggt tgaacaacca
     3481 gccaggacca gcaagcaagg cagaacaaaa ctgataattt tcatttattg atctcacata
     3541 tttatccaga cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat
     3601 tcactcaaat catattacgc cttgtttcat ctttgttgat gatgttttat catgcctgca
     3661 aagattaaat aatcagcatt tacccgccgt atcctggagt tgtttcgtgt cagatcagat
     3721 tatcgcccgc gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca
     3781 gcttctggag atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga
     3841 tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac agccagaaaa tgcacatccc
     3901 ggagaatttt accgtctcgt gggattactc gttatgcaaa cgcgctattg atgaaaactg
     3961 ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg
     4021 aatcaccaca tttctgagta caccaattca cttaccggat ggatcattct atggcacgct
     4081 ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt
     4141 attcgccgga ctgattgcac aatatattca aaaagaggcg ttggttgaac agctgcgcga
     4201 agccaatgcc gcgctgattg cgcaatcgta taccgactcg ttaaccgggc taccgaatcg
     4261 gcgggcgatt tttgaaaatc tgacgacgct gttttctctc gcccggcatc ttaaccataa
     4321 gataatgatc gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca
     4381 taatagtggc gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa
     4441 tggcgaagtt attggtcgtc tcggcggtga tgagtttttg gttgtttcac taaataacga
     4501 gaatgcggat atttcgtcgc tgcgagaacg tattcaacag caaatacgtg gagaatatca
     4561 cttaggtgat gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc
     4621 tgaaacaacc gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa
     4681 aaaacacaaa cagaaaacac cttttgtcgc gcatccagcg ctacattcct gaggcgtatt
     4741 cacatccttt tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttt
     4801 attacagcgg tcgtattttt agcatggttt tttattggcg gctatgctgc cccgggagca
     4861 taaagatgaa aaaaacaacg attattatga tgggtgtggc gattattgtc gttctcggca
     4921 ctgagctggg atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc
     4981 ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc
     5041 accaataacg gcaaccacga agctgccaaa attgaagcca tcgactttac caaagccaaa
     5101 cagtgtgctg atccatccgc cgactacggc accgactatc cccagcagaa tagtcataaa
     5161 gaatccacca ccatctttac ctggcatgat ccacttcgcc agaataccgg caataagccc
     5221 aaaaataatc catgacagaa tgcccattgt ttcctcactt atctgttttg cattagcggg
     5281 ttagtcgctg ataaaaagca tagcacaaca tcgggagggc aagatttgtg acgagcatca
     5341 cggaggattt ttttgcgatg gcgcagaaat tgcgccatca acgatcggtg ataattacca
     5401 accacaaaca tcatgttcgt tttccgtgtc ttgacatcct ccacgccctg aatgacgagg
     5461 atccctgct
//
LOCUS       DAEVZA010000221         5419 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEVZA010000221 DAEVZA010000000
VERSION     DAEVZA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.221"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..319)
                     /locus_tag="LAF92_004853"
     CDS             complement(<1..319)
                     /locus_tag="LAF92_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBI9793740.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLL"
     gene            complement(319..870)
                     /gene="kefG"
                     /locus_tag="LAF92_004854"
     CDS             complement(319..870)
                     /gene="kefG"
                     /locus_tag="LAF92_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBI9793741.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            1001..2914
                     /locus_tag="LAF92_004855"
     CDS             1001..2914
                     /locus_tag="LAF92_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI9793742.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            2914..3936
                     /locus_tag="LAF92_004856"
     CDS             2914..3936
                     /locus_tag="LAF92_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI9793743.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            3930..4148
                     /locus_tag="LAF92_004857"
     CDS             3930..4148
                     /locus_tag="LAF92_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBI9793744.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            4202..5071
                     /locus_tag="LAF92_004858"
     CDS             4202..5071
                     /locus_tag="LAF92_004858"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBI9793745.1"
                     /translation="MSAQHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(5126..>5419)
                     /locus_tag="LAF92_004859"
     CDS             complement(5126..>5419)
                     /locus_tag="LAF92_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBI9793746.1"
                     /translation="EMVLMAAGGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRL
                     FTHINLHFIVTGRDLKDAAVARAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         1349 a         1503 c         1423 g         1144 t
ORIGIN      
        1 tcaataatcc cgccagcaac gccgcgctta acagcacctg tgccgcgcct acgccaaaaa
       61 tcgaacgccg cagttgccaa agtttggagg gattcaactc aagaccgatg ataaacatca
      121 ggaataccac gccgagttcc gaaaagtgga ggatctcatc gacgtcgcta ataaacccca
      181 gcccccatgg accaattgca attcctgcca gcaaatatcc caacacagcg ccaataccca
      241 gccgcgatgc cagcggcacc gcagccaccg ccgcgaagag aaacagcact cctgcgagta
      301 aaaaatcgga accttccatc agcggcctcc tggagacagc ggatttgcca gccagtcacc
      361 gtaggctctg gcgtgactcg ccagctcctg tgcgctttgc cgtctcgccc agtaaataat
      421 gatgggactt aaccaatgca tccggcacat gcccgccgcc agttcaaagg gacgcagaac
      481 atcgctcatc gggtagcgat tcaatgcgtc ataacggtaa gcactttccg gctcgccggt
      541 ggtaatcacg ctacgccagt actttcccgc cagttggttt cctcccggcc cgctggcaaa
      601 accacgactt aatacccggt ccagccactc tttcagtagc gccgggcagc tataggtata
      661 aagaggatgc tgaaagacaa tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat
      721 atcaataaaa aaatcgggat agtgcgcata aaggtcgtgc acggtaacat tgctgagctg
      781 cgtggccggt ttaagcagta cccggtttgc caccgagtcc tgagattccg gatgggcata
      841 cagcagcaaa actttcgctg gctgagacat catccccctc ccggtatggt ttttgtgtat
      901 agtcgctgtt ttgggctacc attgcgcccg gtgcggcagc tcgcccatac attacattat
      961 cataatgata agttaacata gtctgaacat acggcgcctt atgattgttt tctcctcgtt
     1021 acaaattcgt cgcggcgtgc gcgtcctgct ggataatgcc accgccacca tcaaccctgg
     1081 gcagaaagtc ggcctggtgg gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct
     1141 gaaaaatgaa atcagcgccg acggcggcag ctacaccttt ccgggaagct ggcaactggc
     1201 gtgggtgaat caggaaacgc cggcgttacc gcaagcggcg ctggaatatg tcattgacgg
     1261 cgaccgtgaa tatcgtcaac tagaagcgca gctacacgac gccaacgaac gtaacgacgg
     1321 gcacgccatt gcgaccattc atggcaagct ggatgctatt gacgcatgga gtattcgctc
     1381 ccgtgccgcc agcctgctgc acggcctcgg tttcagcaat gaacaactgg agcgcccggt
     1441 aagtgatttc tccggtggct ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg
     1501 ttcagacttg ctgctgctcg acgaaccgac taaccacctc gatctcgatg ccgttatctg
     1561 gctggaaaaa tggttgaaga gctatcaggg cacgctgatc ctgatctctc acgaccgcga
     1621 cttcctcgat ccgatcgttg ataaaattat tcatatcgaa caacaaagca tgttcgagta
     1681 caccggcaac tacagttcgt ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc
     1741 gatgtatgaa agccagcagg aacgcgtagc gcatctgcaa agttatatcg accgtttccg
     1801 tgccaaagcc accaaagcga agcaggccca gagccgcatt aagatgctcg agcgtatgga
     1861 gctgattgcc cccgcgcacg tcgacaaccc gttccgcttt agcttccgcg cgccggaaag
     1921 cctgccaaat ccgttactga agatggaaaa agtcagcgcg ggctatggcg atcgcattat
     1981 tctcgactcg attaaactga acctggtgcc cggctcgcgc attggtctgt taggccgcaa
     2041 cggcgcgggt aaatcgacat taatcaaact gttagccggt gaacttgcgc cagtcagcgg
     2101 tgaaattggt ctggcgaaag ggatcaagct cggctacttc gcccagcatc aacttgaata
     2161 cctgcgcgcc gacgaatcac ctattcaaca tctggcacgt ttagcgccgc aggagctgga
     2221 acaaaaactg cgtgactacc tcggcggctt tggtttccag ggcgataaag taaccgaaga
     2281 aacgcgccgc ttctcaggtg gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca
     2341 gcgtccgaat ctgctgctgc tcgacgaacc gactaaccac cttgacctcg acatgcgtca
     2401 ggcactcacc gaagcattaa tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg
     2461 tcatttgctg cgttccacca ctgacgatct ctacctggtt cacgatcgta aagtcgaacc
     2521 gttcgacggc gatctggaag attatcaaca gtggttgagc gacgtacaaa agcaggaaaa
     2581 ccagaccgac gaagcgccaa aagagaacgc gaacagcgcc caggcacgta aagatcagaa
     2641 gcgtcgggaa gcggagctgc gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct
     2701 ggaaaaagag atggagaagc tgaacgcgca actggcgcag gcggaagaga aactcggcga
     2761 cagcgaactg tatgaccaga gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc
     2821 cagcgccaaa tccggcctgg aagagtgcga aatggcgtgg ctggaagccc aggagcagct
     2881 tgagcagatg ctgctggaag gccaaagcaa ctgatggcgc agataacgac gaccgatgcc
     2941 aatgaattta gcagcagtgc tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta
     3001 caaactatgc tgccgcgttt gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg
     3061 ctggagttgc ccgacggcga ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg
     3121 aaacataaac cgcgtttagt ggtgtttcac gggctggaag gcagtctcaa tagcccttac
     3181 gcccacggtc tggttgaggc ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt
     3241 cgcggatgca gcggtgaacc aaaccgtatg caccgcattt accattcggg cgaaaccgaa
     3301 gacgccagtt ggtttttacg ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc
     3361 gtcggctatt cgctcggcgg taatatgctg gcctgtttgc tggcaaagga aggcaatgat
     3421 ctcccggttg atgcggcggt gattgtctct gcgccgttta tgctggaagc ctgtagctat
     3481 catatggaaa agggcttttc ccgcgtttat cagcgttact tgctgaacct gttaaaagcc
     3541 aatgccgcgc gcaagctggc agcctacccc ggaacgctgc cgattaatct cgcgcagtta
     3601 aaatcggtac gtcgcatccg tgaatttgac gatctcatca ccgccagaat tcacggctac
     3661 gccgacgcta tcgactatta tcgtcagtgt agcgccatgc cgatgctgaa ccggatcgcc
     3721 aaaccgacgc tgattattca cgccaaagac gatccgttta tggatcatca ggtgatcccg
     3781 aaaccggaaa gtctcccccc gcaggtggag tatcaactga ctgaacatgg cggtcatgtt
     3841 ggctttattg gcggtacatt acttcatccg caaatgtggc tggagtcacg cattcctgac
     3901 tggttaacaa cgtatctgga ggcgaaatca tgttgattcc gtggcaagac ctctcccccg
     3961 aaacgctgga aaatttaatt gaaagctttg tgttacgtga aggcaccgat tatggtgaac
     4021 atgagcgtac acttgaacag aaagtcgccg acgtcaaacg ccagctacag tgcggagaag
     4081 cggtgctggt atggtcggag ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc
     4141 gcgaataacc tgcaaccgtc ggcggacgag ataataatga tagctaagtc atggagttac
     4201 tatgtctgcc caacatccgg tcattgcggt aacaggatcc agcggcgcgg ggaccaccac
     4261 caccagcctc gcgtttcgta aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga
     4321 aggcgacagt tttcaccgtt acacccgccc ggaaatggac atggcgatcc gcaaagcgcg
     4381 cgacgccggg cggcatatca gctacttcgg ccccgaagcg aacgacttcg gcctgctgga
     4441 acaaaccttc attgaatacg gtcagagcgg caaagggaaa tctcgcaaat atctgcatac
     4501 ctacgatgaa gccgtaccgt ggaatcaggt accgggaaca ttcaccccct ggcaaccttt
     4561 accagaaccc actgatgtac tgttttatga aggtttacac ggcggcgtag tcacgccaca
     4621 gcataacgtt gcgcagcatg tggacttact ggtcggcgtg gtgcctatcg ttaaccttga
     4681 gtggattcaa aaactgatcc gcgacaccag cgagcgcggg cactcacgag aagcagtgat
     4741 ggactcagta gtgcgttcaa tggaagacta tatcaactac atcacaccgc agttttcccg
     4801 cacccatctt aacttccagc gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa
     4861 aggtatcccg tcgctggatg aaagctttgt ggtgatccat tttcgtaatc tggaagggat
     4921 cgatttcccc tggctgctgg cgatgttgca aggctcattc atttcccaca tcaatacgtt
     4981 agtggtaccg ggcggcaaaa tggggctggc aatggaatta attatgctgc cgctggtgca
     5041 acgattgatg gaaggaaaga aaatcgagta actctgctat tacgccggat aaaatactat
     5101 ccggcttcac aacgggatag ttaaatcacg cggcaaccac ttcatacgag tgagtaatat
     5161 tcaccgcttt ttccagcatc aacgccactg aacaatattt ctcagcagag agatcaaccg
     5221 cacgcgcaac cgctgcgtct ttcaggtcgc gaccggtgac gataaaatgc agattaatgt
     5281 gcgtaaacag gcgtggtgcc tcttcgcggc gttcagaggt caattttact tcacaatcga
     5341 ccacatcctg acgccctttt tgcaggatag aaaccacatc gatggcactg caaccccccg
     5401 ccgccatcaa caccatttc
//
LOCUS       DAEVZA010000222         5404 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEVZA010000222 DAEVZA010000000
VERSION     DAEVZA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.222"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..505)
                     /gene="tapT"
                     /locus_tag="LAF92_004860"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(<1..505)
                     /gene="tapT"
                     /locus_tag="LAF92_004860"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBI9793747.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTDPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSA"
     gene            complement(574..993)
                     /gene="trxC"
                     /locus_tag="LAF92_004861"
     CDS             complement(574..993)
                     /gene="trxC"
                     /locus_tag="LAF92_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBI9793748.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            1200..2237
                     /locus_tag="LAF92_004862"
     CDS             1200..2237
                     /locus_tag="LAF92_004862"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBI9793749.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(2285..2974)
                     /gene="ung"
                     /locus_tag="LAF92_004863"
     CDS             complement(2285..2974)
                     /gene="ung"
                     /locus_tag="LAF92_004863"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBI9793750.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            3279..3662
                     /gene="grcA"
                     /locus_tag="LAF92_004864"
     CDS             3279..3662
                     /gene="grcA"
                     /locus_tag="LAF92_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBI9793751.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(3718..4305)
                     /gene="eamB"
                     /locus_tag="LAF92_004865"
     CDS             complement(3718..4305)
                     /gene="eamB"
                     /locus_tag="LAF92_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBI9793752.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            4408..5289
                     /locus_tag="LAF92_004866"
     CDS             4408..5289
                     /locus_tag="LAF92_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9793753.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         1422 a         1296 c         1431 g         1255 t
ORIGIN      
        1 aggcagaaag ccgggaaaga tcgacggaaa tgacgggaag attatccaga tacggacttt
       61 tacgaaacat cttgcgcgct tccggccagg taccatcgag catgataaac agcggtggct
      121 taccggcagg tggtgtgaag atcacttccc gttgctcatc agcatacgag gcgggaaaga
      181 ccaccattgg ttgatagtcc gggttttgta ccagatccag caagtcttgc gaggggtcgg
      241 tacgcgacca ttgaaacgca acggtatcag gcaaaatatc agcaatcaga cgcccggtgt
      301 tacttggctt cattggctcg gtgtcgaaca tcagcaaaca gaagcgactt tttgcttgtg
      361 ctggggtaat tgtcgaacag agacataatt tctctggcaa aagacagcgt tggcagcgac
      421 gaacgcgatt accgcgggca agaaaaggac gtgttgcgcg cgcaatacgc tcggcgcgta
      481 actggagaac agcgttttcg gtcataagag agcgtcgaaa aaacgccatt gtcgcagagg
      541 agaaaacggg gcacaagatg tgccccggta agattaaaga gattcgttca gccagctatc
      601 gaacggcgct ttcggtactg cgccattaag catgtcgaca acctgaccgt ttttgaaaat
      661 catgatcgtc ggtatactac gaattccaaa gcgactgctc aattcacgtt cagcttcggt
      721 attcactttc acaaagcgca ctttaccgct acgctcttgc gcgacatctt caaaaattgg
      781 tgcgaaatta cggcaggggc cgcaccacgg tgcccagaag tcgatcacca caggtagatc
      841 atccttcagc aatttgtcga gtgtttcacc ggtcgcatta atcacctctc cgtcaaacaa
      901 gtcgtgaccg cagcgtccgc attttgccgc atcttcgatc cgatcgtcgg gaatgcgatt
      961 gatggcctga caatgggtac aaacggtatt cataactaac ctcgggatga gtaaggagac
     1021 ttatcggcaa cgacgccggt atgtttctaa tatgttacat attatcgggg acattgttcc
     1081 agacaacaat ctgttttatt caataggtat gatagtcgca ggctttggta cgaaatcatg
     1141 gcgaaccgcg cggacttcgg gtaatctgcg cgcttcgcgc agcgctggtg gagaaaagca
     1201 tgaacgatga aatgaaaggt aaaagcggca aggtcaaagt gatgtatgtc cgcagtgatg
     1261 atgattctga taaacgtacc cacaacccgc gtaccgggaa agggggcggg cgtccaggaa
     1321 aatctcgtgc tgacggtggc cgtcgccccg cccgcgatga caaacagagt cagccccgtg
     1381 accgcaagtg ggaagattcg ccgtggcgca cggtttcccg cgcgccgggt gatgagacgc
     1441 cggaaaaggc cgatcacggt ggcatcagtg gtaaaagttt tattgatccg gaagtgttgc
     1501 gtcgtcagcg tgcggaagaa acccgcgtct acggcgaaaa tgcatgtcag gcacttttcc
     1561 agagccgtcc ggaagcgatt gttcgcgcct ggtttatcca gagtgtaacg ccgcgtttta
     1621 aagaagcctt gcgctggatg gcagcaaacc gcaaagcgta ccatgtggtg gatgaagcgg
     1681 aattgacaaa agcgtcaggc acggaacatc acggcggcgt ttgcttcttg atcaaaaagc
     1741 gtaacggtac aaccgtgcag cagtgggtaa gccaggcagg cgcgcaggat tgcgttctgg
     1801 cactggaaaa cgaatctaac ccgcataacc tgggtggcat gatgcgcagc tgcgcgcact
     1861 tcggcgtgaa aggtgtcgtg gtgcaggatg cggcactgct ggagtcgggg gcggctatcc
     1921 gtaccgcaga aggcggcgca gagcacgttc agccgattac tggcgacaac attgttaacg
     1981 tgctggatga tttccgtcag gcaggttaca ccgtagtgac cacttccagc gagcagggta
     2041 aaccgctgtt caaaactagt ctgccagcga aaatggtact ggtgctgggt caggaatatg
     2101 aagggttacc ggatgccgca cgcgatccga acgatctgcg cgtgaagatt gacggtacgg
     2161 gcaacgttgc cgggctgaat atctctgtcg caaccggcgt tttgcttggt gaatggtggc
     2221 gtcagaataa agcctgataa atcagccggg tggcaactct gccatccggc atttccccgc
     2281 aaatttactc actctctgcc ggtaatactg gcatccagtc aatcggcgtc tcgccacgtt
     2341 gttccagcca ctgatttgcc agcacaaaat ggttgcagcc aaagaatcca cgatgcgccg
     2401 aaagcggcga cggatgcggt gctttcagta catgatggcg ttgcttatct ataatcgccc
     2461 ctttcttttg cgcatgtgat ccccacaaca aaaacaccac gccttcgcgg tgctggttaa
     2521 tcaggctgat gactttatca gtaaacgtct cccaaccgag gctggcgtgg gaatgcgcct
     2581 gacctgcgcg aaccgtcaac acagtattga gcagcagaac gccctgacgc gcccagcttt
     2641 cgagatagcc atgattaggg cgggtgaagc ccggaatagt attttccagc tctttataca
     2701 tattcaataa tgacggagga atggcaatgc cgggacgaac ggaaaatgcc agaccatgcg
     2761 cctgtcccgg tccgtgataa ggatcctggc cgagaatcac cactttaacg tcacccaact
     2821 cggtaaagcg gaacgcgtta aagacatctt tttgtggtgg atagatagtg acgccggact
     2881 gccgctcgct ggcgacggtc tgaagggtat taagaaaata aggttgttgc ttctcttcag
     2941 ccagcacgtc atgccaggtt aattcgttag ccatctcgct ctcctgcgaa tcttcaatcc
     3001 gcctagctta actgcttctt tctacagaac aaaatcatgc tcatttttta gacttaaacg
     3061 agtgcaaaaa aatgtaaatt ggtaaaattt cacacccgca taagttgatg taaaacaata
     3121 aaatccattt acgaccacaa tcgatgcgtg gtttttattg atttaaatca aagaataaag
     3181 ggtggttgag gagtatatat acactcaagc aacaatggtt ttaccaattg gccgcgacag
     3241 gctgaacaaa tcaaataatt ttgccgggga ggcatcacat gattacaggt atccagatta
     3301 ctaaagccgc taacgacgat ctgctgaact ctttctggct gctggacagc gaaaaaggcg
     3361 aagcacgttg catcgttgca aaagcaggtt atgcagaaga tgaagtggtt gcagtaagca
     3421 aactgggtga cattgaatac cgtgaagttc cagtagaagt gaaaccagaa gttcgcgttg
     3481 aaggtggtca acacctgaac gttaacgttc tgcgtcgtga aactctggaa gatgcagtta
     3541 agcatccgga aaaatatccg cagctgacca tccgtgtatc cggttatgca gttcgcttta
     3601 actctctgac tccggaacag cagcgcgacg ttatcgcccg tacctttact gaaagcctgt
     3661 aaggcttaag tagcaagtta ctaagcggaa gagggcgacc tcttccgctt tttttcgtta
     3721 atagaaaatg cgtaccgcgc aatagaccag caacagggca agcacgatat ttaactggcg
     3781 accatactgg cgaaacaatc gctgaaacag atgccccgcc agcgcccagc acacattgcc
     3841 aaacgtccca atcatcgcca gcaaaacgct gacgccaact acccagctta gcgcctgtgt
     3901 ttgcggcaga acaaacgtcg acagtgccgt aacaccgtac aaaatgattt tgacgttcac
     3961 aaactgcaaa gcaaagctgg cccaaaagct gattggtttt gcctgaagtc cgtcttcctt
     4021 tgttgggctg gtggcgattt tccacgccag ccagacaata tatgccgccc ccgcccaact
     4081 caaaaggtgt accgctgccg ggtcaatcac tgccagtgaa aatgaaatgc ccgcacacag
     4141 taacatcaca atcaaaaatc ccagactcat ccctgccagc acgcgggtac tttgacgaaa
     4201 tccatgcgac gtagcagagc taagggcgag aatattgttc ggtcctggcg tcatagcggt
     4261 aatcagggtg taagtccaaa aagcacttaa aagggtcggt gtcacttgtt atctcctctg
     4321 ttttggtaga cagtacccga cacggaacat ttaaaaaaat gaatgttttt atattctaga
     4381 ttcgatattt tcgataggtt tggggttatg gatctgcgcc gttttattac gcttaaaacc
     4441 gtggtggaag agggttcctt tttgcgagct tcgcaaaaat tgtgctgtac acaatcgacg
     4501 gtgacttttc atattcagca gcttgagcag gagttttcag tccagttatt tgagaaaatt
     4561 ggccgacgga tgtgccttac ccgcgaagga aaaaagttgc tgccgcacat ttatgaactt
     4621 acccgagtga tggatacgct tcgtgaggct gccaaaaagg agtcagatcc ggacggggaa
     4681 ctacgcgttg tttcgggcga aacgttgctc tcttaccgaa tgccgcaggt gttgcagcgt
     4741 tttcggcaac gcgcaccaaa agtgcgttta tcgttgcagt cgctgaactg ttacgtgatc
     4801 cgtgatgcgc tactgaatga tgaggccgat gtcggcgtct tttatcgcgt agggaatgat
     4861 gatgccctga accgacgaga gttgggtgaa caatcgctgg tgctggtggc ttcaccgcaa
     4921 attgcagatg tcgattttac tgagccggga agacataacg cctgtagctt tattatcaac
     4981 gaaccgcaat gtgtcttccg gcagatattt gagagcacgc tgcgtcagcg gcggatcacg
     5041 gtggaaaaca ccattgagct gttaagcatt gaaagtatca aacgttgtgt cgcggcgaat
     5101 atcggtgtca gttacttgcc ccgctttgcg gtggaaaaag agcttgagag cggagaatta
     5161 attgaattgc cttttggcga acagtcgcag accattacgg ccatgtgcgc acaccatgct
     5221 ggaaaagcgg ttagcccggc gatgcacact tttattcagt gtgttgaaga gagttttgtg
     5281 gctggataaa aataatgtcg ggattaaacc cggcatttaa cttgtcgaca aagtgcgctt
     5341 tattcatgcc ggatgcggcg tgaacgcctt atccggccta caagatcgtg caaattcaat
     5401 atat
//
LOCUS       DAEVZA010000223         5398 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEVZA010000223 DAEVZA010000000
VERSION     DAEVZA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.223"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..224
                     /locus_tag="LAF92_004867"
     CDS             <1..224
                     /locus_tag="LAF92_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="with ErmA and TolC forms a multidrug efflux system;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug export protein EmrA"
                     /protein_id="HBI9793754.1"
                     /translation="RLPVRIELDQKQLEQYPLRIGLSTLVSVNTTNRDGQVLANKVRS
                     TPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            241..1779
                     /gene="emrB"
                     /locus_tag="LAF92_004868"
     CDS             241..1779
                     /gene="emrB"
                     /locus_tag="LAF92_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBI9793755.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(1843..2358)
                     /gene="luxS"
                     /locus_tag="LAF92_004869"
     CDS             complement(1843..2358)
                     /gene="luxS"
                     /locus_tag="LAF92_004869"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBI9793756.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(2508..4064)
                     /gene="gshA"
                     /locus_tag="LAF92_004870"
     CDS             complement(2508..4064)
                     /gene="gshA"
                     /locus_tag="LAF92_004870"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBI9793757.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(4137..4565)
                     /locus_tag="LAF92_004871"
     CDS             complement(4137..4565)
                     /locus_tag="LAF92_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI9793758.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(4562..5128)
                     /gene="yqaB"
                     /locus_tag="LAF92_004872"
     CDS             complement(4562..5128)
                     /gene="yqaB"
                     /locus_tag="LAF92_004872"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBI9793759.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         1255 a         1456 c         1346 g         1341 t
ORIGIN      
        1 agcgtctacc tgtgcgtatc gaactggacc agaaacagct ggagcaatac ccgctgcgca
       61 ttggtttgtc cacgctggtg agcgtcaata ccactaaccg tgacggtcag gtactggcaa
      121 ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg tgaaatcagc ctggcacctg
      181 tcaataaact gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg
      241 atgcaacagc aaaaaccgct ggaaggcgcg caactggtca ttatgacgat tgcgctgtca
      301 ctggcgacat tcatgcaggt gctggactcc accattgcta acgtggcgat ccccactatc
      361 gccgggaatc tgggctcatc gctcagccag ggaacgtggg taatcacttc tttcggggtg
      421 gcgaatgcca tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa
      481 ctgttccttt ggtccaccat cgcctttgct attgcgtcgt gggcgtgtgg tgtctccagc
      541 agcctgaata tgctgatctt cttccgcgtg attcagggga ttgtcgccgg gccgttgatc
      601 ccgctttcgc aaagtctatt gctgaataac tacccgccag ccaaacgctc gatcgcgctg
      661 gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct cggcggttat
      721 atcagcgata attaccactg gggctggata ttcttcatca acgtgccgat tggcgtggcg
      781 gtggtgttga tgacactgca aactctgcgc ggacgtgaaa cccgcaccga acggcggcgg
      841 attgatgccg tggggctggc actgctggtt attggtatcg gcagcctgca gattatgctc
      901 gaccgcggta aagagctgga ctggttctca tcacaggaaa ttatcattct taccgtggtg
      961 gcagtggtgg ctatctgctt cctaattgtc tgggagctga ccgacgataa cccgatagtc
     1021 gatctgtcgt tgtttaagtc gcgcaacttc accatcggct gcttgtgtat cagcctcgcg
     1081 tatatgctct acttcggcgc gattgttctg ctgccgcagt tgttgcagga ggtctacggt
     1141 tacacggcaa cgtgggcagg tctggcctct gcgccggtag ggattattcc ggtgatcctg
     1201 tcgccgatta tcggccgctt cgcgcataaa ctggatatgc ggcggctggt aaccttcagc
     1261 tttattatgt atgccgtctg cttctactgg cgtgcctata cctttgaacc aggtatggat
     1321 tttggcgcgt cggcctggcc gcagtttatc caggggtttg cggtggcctg cttctttatg
     1381 ccgctgacca ccattacgct gtctggtttg ccaccggaac gactggcggc ggcatcgagc
     1441 ctctccaact ttacgcgtac gctggcgggg tctatcggca cgtccatcac caccaccatg
     1501 tggaccaacc gcgagtcgat gcaccacgca cagttaaccg agtcggtaaa cccgtttaac
     1561 ccaaacgccc aggcgatgta cagtcaactg gaagggcttg ggatgacgca acagcaggcc
     1621 tccggctgga ttgcccagca gatcaccaac caggggctga ttatttccgc caatgagatc
     1681 ttctggatgt cagccgggat attcctcgtc ctgctggggc tggtgtggtt tgctaaaccg
     1741 ccatttggcg caggtggcgg cggaggcggt gcgcactaag tacaactaag ccagttcatt
     1801 tgaactggct tttttcaatt aattgtgaag atagtttact gactaaatgt gcagttcctg
     1861 caacttctct tccggcagcg ccagttcttc gttgctgttg atgcgtacgt cacgttccag
     1921 aatgctacgc gcaatatcct gcgcttcctg caacgagtgc atctggtaag tgccacactg
     1981 gtagacgttc agttccggga tctgattctg atcctgcact ttcagcacgt cttccattgc
     2041 cgctttccag gcatcagcaa cacgctgctc atctggcgta ccaatcagac tcatataaaa
     2101 accggtgcgg cagcccattg gcgagatatc gataatctct acaccattac cgttaagatg
     2161 gttacgcata aaaccagcaa acaggtgctc cagggtatgg atccctcttt ctggcatcac
     2221 ttctttgttc ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc catgcggggt
     2281 gttcattgtt ttcgccaccc gaactgcagg cgcttccatc cgggtatgat cgactgtgaa
     2341 gctatctaac aacggcattt agccacctcc ggtaattttt ttaaaaattt tctgaactct
     2401 ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc atccctgaat
     2461 tcagagatga aattttggcc actcacgagt ggcctttttc ttttctgtca ggcgtgtttt
     2521 tccagccaca ccgcaaacgg ttcggtatca gcggcttcca tttcctgctg acggcgttca
     2581 gacgcctcgc gctcggctac aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc
     2641 agcagattac ggtaggcttc tgcaaatgct ttgcctgttc cgccaatacc agtatcaatc
     2701 atagacctta agatacgggc agagaaagtc agatcgggat tatcgaagca ggcaaccagt
     2761 tcatcacaca ctttctgata cgcttcgccg ccgttaatac tatccagcgt ttgcgcgacg
     2821 cgtttcagat cgcggaacag atctttaccc acctgcggta acgggaactg tgcggtttcg
     2881 cagccgatac ccagcgtcag acccggtttg cgaccttcga ggatcacccg gttccagtta
     2941 acgcgtgtac aggcaagttc gctactgctc atttccggtg catcagccag cgcacaccag
     3001 accataaaca ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg cgagaacggg
     3061 ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa
     3121 ggtgactcgc cgctgcgggt aacgcgtttt ggacgaatcg gcgcatacag ttcgttttca
     3181 atctgcagca cgttgctatt gatttgcagc ctcttaccgt ctttctcaat accaatcttc
     3241 gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc ccgctacgta ctcgtaaaga
     3301 tcgttgaagg taataccaag attgctttgc gatttattgg tatagccgag atcgctcaaa
     3361 cgaagagagg tcgcatacgg caggtaatac ataccgcact cggttttctc aaacggcagc
     3421 gatgttggtt ttccttgcag gaaagaagaa caaatcgccg gagatgcacc aaacagataa
     3481 ggaatgaccc aaccgaaacg atagtaattg cggataacgc ggaaatagcc cgcagaaatt
     3541 ttctctttgg catcagcgcc cgagatatca ccgcacttcg cttgccagaa tgccattggt
     3601 aaagagaaat tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta gcgatttttc
     3661 agcccttcac gatacagcgt tttaaagcgt ccggtgttag aagtgccgta ctgtgccagt
     3721 tcgatgtcct gaccttctgc gatgtagcat ggcatactta acggccacat ccgctcatcg
     3781 cccatattgc gcgccgtata acgatgcaga tcgcgcataa aggtcagcat atgttcaata
     3841 tcaccatcca ctggtgtaat gaattccagc aatgcttccg caaaatcggt agtaatccat
     3901 ttatgcgtca gtgcggaacc taatgcttca ggatgacctg ttgttgccag tgtgccatca
     3961 gcattaacac gcaaagtttc gcgctccagc ccacgctgta tcccctttaa cgcctgagga
     4021 tgtttttcca gccaggccag cgcctgtgat acgtccggga tcaaattgac ctcccgcctg
     4081 tcaaaatcgt tttaattagc ataactgtaa tggtgaccat atgtgcaggc ctacaattag
     4141 tgccaccaca tcatgccctg aacggtcgct gctgcaactg caacatagcg taacgcttta
     4201 ccaaggcaca aaaaaaagat tactggcccc caggagatgc gcatccatcc cgctaacaga
     4261 cacagtaaat cgccaaccac gggcatccag cttaataata gcgtgactgc accatagcgt
     4321 ttcagccagc cggtagcttt ctcttgccag cgcgatgttt tacgcaatgg aaagaaacgc
     4381 ccaaggataa cgttagttaa ccctccaagg ctattaccca ttgttgctgt taaaactaaa
     4441 acccagggat aactgatccc ggaaagcaac attgccacca gcacgacttc ggagttgccg
     4501 ggtaatagtg tagcgctgag aaaactactg gcaaacaacg agaaaagcga taacgcttca
     4561 ctcacagcaa gcgaacatcc acggcgtcca tgcctgctgc acgggccgcc tgaataccga
     4621 aatcggcatc ttcaaagacc acacactgcg tcggttgcac gcccatacgc tgcgcgcaca
     4681 acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg atcagcagcg acgacggcgt
     4741 caaaataacg gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg ctttcactcc
     4801 ccgttcctac agccattggg cgacgaccat gccaactttt caccacttca acaagaggaa
     4861 gcggctcgac actatccagc agcatacttc ttactgcttc tgttttttca cgcgctaacg
     4921 catgcgggtc gagatcggcc tgattcagct caataattgc ctgagcaata cgccaggtgg
     4981 gcgatccatt aagcgcaatc attgcctgaa catcgtactg aagaccgtag tgccctaata
     5041 cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag gattgtgcca tccatatcaa
     5101 aaattaaacc tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta
     5161 ctttagcgta attgcttgat tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg
     5221 gataaagtag ttatttagaa atgagatatt tttgaaggaa atttttggga gaagatggtg
     5281 catccgggag gattactcac tggcgttcgc ccttcgggcc gttgctgacg caacgttatc
     5341 ctccctggtg cctgcggtta ataccgctat ctcttcgata ccttcattgc tgaaggtt
//
LOCUS       DAEVZA010000224         5379 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEVZA010000224 DAEVZA010000000
VERSION     DAEVZA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.224"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..694
                     /gene="glnH"
                     /locus_tag="LAF92_004873"
     CDS             <1..694
                     /gene="glnH"
                     /locus_tag="LAF92_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBI9793760.1"
                     /translation="SSHAADKKLVVATDTAFVPFEFKQGDKYVGFDVDLWAAIAKELK
                     LDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAIDFSDGYYKSGLLVMVKANN
                     NDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPNIDNAYMELGTNRADAVLH
                     DTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDELRDKVNGALKTLRENGTYN
                     EIYKKWFGTEPK"
     gene            833..1492
                     /gene="glnP"
                     /locus_tag="LAF92_004874"
     CDS             833..1492
                     /gene="glnP"
                     /locus_tag="LAF92_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBI9793761.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            1489..2211
                     /gene="glnQ"
                     /locus_tag="LAF92_004875"
     CDS             1489..2211
                     /gene="glnQ"
                     /locus_tag="LAF92_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBI9793762.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            2328..4553
                     /gene="ybiO"
                     /locus_tag="LAF92_004876"
     CDS             2328..4553
                     /gene="ybiO"
                     /locus_tag="LAF92_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBI9793763.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(4550..>5379)
                     /gene="rlmF"
                     /locus_tag="LAF92_004877"
     CDS             complement(4550..>5379)
                     /gene="rlmF"
                     /locus_tag="LAF92_004877"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBI9793764.1"
                     /translation="LTLTPAGEQSVDFANPLAVKALNKALLAHFYAVANWDIPDGFLC
                     PPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSET
                     SSQALSSAQAIISANPGLNRAIRLRRQKESGAIFNGIIHKNEQYDATLCNPPFHDSAA
                     AARAGSERKRRNLGLNKDDALNFGGQQQELWCEGGEVAFIKKMIEESKGFAKQVMWFT
                     SLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
BASE COUNT         1211 a         1417 c         1431 g         1320 t
ORIGIN      
        1 ttcttctcat gccgcggata aaaaattagt tgtcgcgacg gataccgcct tcgttccgtt
       61 tgaatttaaa cagggcgata aatatgtggg ctttgacgtt gatctgtggg cggccatcgc
      121 taaagagctg aagctggatt acgaactgaa gccgatggat ttcagtggga tcattccggc
      181 actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt accatcaccg acgagcgtaa
      241 aaaagcgatc gatttctctg acggctacta caaaagcggc ctgttagtga tggtgaaagc
      301 taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg aaagtggttg ctgtgaagag
      361 cggtactggc tccgttgatt acgcgaaagc aaacatcaaa actaaagatc tgcgtcagtt
      421 cccgaacatc gataacgcct atatggaact gggcaccaac cgcgcagacg ccgttctgca
      481 cgatacgcca aacattctgt acttcatcaa aaccgccggt aacggtcagt tcaaagcggt
      541 aggtgactct ctggaagcgc agcaatacgg tattgcgttc ccgaaaggta gcgacgagct
      601 gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag aacggaactt acaacgaaat
      661 ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc tacacctgta aaacgcactg
      721 gcagttccct ctcccctctg gggagaggat tagggtgagg ggcgcaaacc cgctccgggg
      781 ccattaatta ccctgaattt gattatttac accacggtaa caggaacaac atatgcagtt
      841 tgactggagt gccatctggc ctgccattcc gcttctgatt gaaggtgcca aaatgaccct
      901 gtggatttcg gtcctcggtc tggcaggcgg tctggtaatc ggattgctgg caggttttgc
      961 acgcaccttc ggtggttgga tagccaacca cgtcgcgctg gtctttattg aagtgatccg
     1021 cggcacacct atcgtcgtcc aggtgatgtt tatctatttc gccctgccga tggcgtttaa
     1081 cgacttacgc atcgacccat ttactgcggc ggtggtcacc atcatgatca actccggcgc
     1141 gtacattgcg gaaatcacgc gtggtgcggt gctgtctatt cacaaaggtt ttcgtgaagc
     1201 aggactggcg ctcggtcttt cacgttggga aaccattcgc tacgtcattt taccgctggc
     1261 actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc atcagcatta aagacacctc
     1321 gctgtttatt gttatcggcg tggcggaact gacccgtcag gggcaagaaa ttattgccgg
     1381 taacttccgc gcccttgaga tctggagcgc cgtggcggtg ttctatctga ttattaccct
     1441 ggtgctgagc tttattctgc gtcgtctgga aagaaggatg aaaatcctgt gattgaattt
     1501 aaaaacgtct ccaagcattt tggtccaacc caggtgctgc acaatatcga tttgaacatt
     1561 gctcagggcg aagtcgtggt gattatcggg ccgtccggtt ccggtaaatc gaccctgctg
     1621 cgctgcatca acaaactgga agaaatcacc tccggcgatc tgattgtcga tggcctgaag
     1681 gttaacgatc cgaaagttga cgagcgcctg attcgccagg aagcgggtat ggtgttccag
     1741 cagttttacc tcttcccgca tctgacggcg ctggaaaacg tcatgtttgg tccgctacgc
     1801 gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc gtgagctgct ggcgaaagtc
     1861 ggtctggcag aacgtgcaca tcactaccct tccgaacttt ctggtggtca acagcagcgt
     1921 gtggcgattg cccgcgcgct ggcggtgaag ccgaaaatga tgctgtttga tgaaccgact
     1981 tccgctcttg acccggaact gcgccatgaa gtgctgaagg ttatgcagga tctggctgaa
     2041 gaagggatga cgatggtgat cgtgacccac gaaatcggtt ttgccgagaa agtagcttcg
     2101 cggctgatct ttatcgacaa aggccggatt gcggaagatg gcgatccgca ggtattgatc
     2161 aagaacccgc cgagccagcg cttgcaggaa tttttgcagc acgtctctta acttctctgc
     2221 ctccctttca cccgaaaggg aggcacacca gattcctctc atttaaaatc gcccctcccc
     2281 cagcatctat acttatcttt ttgctctgtt ttctcactgg aggagtcatg cggtggatcc
     2341 tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc ggtatccata cccggcgtta
     2401 caaccacaac gacaacggac tcaacgactg aaccggcccc ggaaccggat atcgaacaaa
     2461 aaaaagcggc ctatggcgca ctggcggatg tgctggataa cgacacctcg cgtaaagaat
     2521 tgatcgacca gttgcgtact gtcgccgcga cgccccctgc ggaaccggtc ccgaagatcg
     2581 tgccgccgac gctggttgaa gagcaaaccg tgctgcaaaa agtcaccgaa gtcagccgcc
     2641 attatggtga agccctttcc gcccgcttcg gacaacttta tcgcaatatc accggctccc
     2701 cgcataagcc gtttaatcca caaaccttca gcaatgcgtt gacccatttt tcaatgttag
     2761 cggtattagt gtttggtttt tactggctga ttcgtctgtg cgcactgccg ctgtatcgca
     2821 aaatgggcca gtgggcgcgg caaaaaaatc gcgaacgcag caactggctg cagcttccgg
     2881 cgatgattat cggggcgttt attatcgacc tactgttact ggcactgaca ttgtttgtcg
     2941 gccaggtatt aagcgacaac ctgaatgcgg gcagtcgcac catcgctttc caacaaagtt
     3001 tgtttctcaa cgcctttgcc ctgattgaat ttttcaaagc tgtactacgc ctgatttttt
     3061 gcccaaatgt ggcggagctg cgcccgttca cgattcagga cgagagcgcc cgttactgga
     3121 gtcgtcgcct gagctggtta agcagcctga ttggctatgg cctgattgtg gccgtgccga
     3181 ttatctctaa tcaggtgaat gtacagatag gtgcgctggc gaacgtcatc attatgctgt
     3241 gcatgaccgt ctgggcgttg tacctgatct tccgaaataa aaaagagatt acccaacatt
     3301 tgctcaactt tgcggagcat tcgctggcct ttttcagcct gtttatccgc gcctttgcgc
     3361 tggtgtggca ctggctggca agcgcctatt ttatcgtgct gtttttcttt tcgttgttcg
     3421 atccgggcaa cagcctgaaa tttatgatgg gtgcaacggt gcgcagcctg gcgattattg
     3481 gtatcgcagc gtttgtttcc ggtatgtttt cccgctggct ggcaaaaacc atcactctct
     3541 cgccacatac tcagcgtaac tatccggagt tgcaaaaacg gctgaatggc tggctttcgg
     3601 cggcactgaa aacggcgcgt attctgaccg tctgcgtggc ggtaatgttg ctgttgagcg
     3661 catggggatt gttcgatttc tggaactggc tgcaaaacgg cgcggggcag aaaaccgtag
     3721 atatcctgat ccgcatcgca ctcattcttt tcttctcggc ggttggctgg acagtgctcg
     3781 ccagtttgat cgaaaaccgg ctggcttcgg atattcatgg ccgcccgcta cccagcgccc
     3841 gtacgcgtac cctgctgacg ctgttccgta acgcgctggc ggtgattatc agtaccatca
     3901 ccatcatgat tgtgttgtcg gaaatcggcg tcaatatcgc gccattgctg gcaggtgctg
     3961 gggcattagg tctggctatc tcgtttggtt cgcaaacgct ggtgaaagat attatcaccg
     4021 gggtatttat tcagtttgaa aacggcatga acactggaga tttggttact atcgggccgt
     4081 tgaccggcac tgtggaacgg atgtcgattc gctccgtggg cgtgcgtcag gataccgggg
     4141 cgtatcacat cattccgtgg tcttcgataa ccacctttgc taacttcgtc cgcggcattg
     4201 gttcggtggt ggcaaactac gatgttgatc gccatgaaga tgctgataaa gccaatcagg
     4261 cactgaaaga tgcggtagcg gaattgatgg aaaacgaaga aattcgcggg ctggttattg
     4321 gtgaaccaaa ctttgccggg attgtcggct taagcaatac cgcgtttaca ctgcgtgttt
     4381 cgttcaccac gctgccactc aaacagtgga cggtccgctt tgccctcgac agccaggtga
     4441 aaaagcattt cgacctggca ggtgttcgcg cgccagtgca gacttatcag gtgctgcctg
     4501 ctccgggcgc ggccccggct gaaccgctgc cgccggggga gccaacgctt taacgttggc
     4561 gattaacaaa acggcggcgc tgctcgtcgt tcataaaggt ccaggcaata aagcgactct
     4621 gcttttgccc ctgggccatc tcttttttaa ccaccttcac cgcgcccacg tccgtcagag
     4681 cacgatacaa cggcggtaag ttttcaccac gagaaaccag cgaggtaaac cacatcacct
     4741 gcttcgcgaa gcctttgctc tcttcaatca tctttttgat aaaggcgact tcaccacctt
     4801 cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc atcgtctttg ttcagcccca
     4861 ggttacggcg tttacgctca ctacctgccc gcgctgcagc ggcggaatcg tggaatggcg
     4921 ggttacacaa ggtcgcgtcg tattgctcgt ttttatggat gatgccgtta aaaattgccc
     4981 cactctcttt ttgccgacgc agacgaatgg cgcggttaag ccccggatta gcactgataa
     5041 tcgcctgcgc actgcttaac gcctggctgc tggtttcact accggtaaaa cgccagccat
     5101 attcatgtac gccaatcagc ggataaatac agttcgcacc aacgccgata tccagaatgc
     5161 tggcattcgc tggaattgtt ccgcttgctt cagccagtaa atcggcaagg tgatgaatat
     5221 aatccgcccg gcccggtact ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta
     5281 cggcgtaaaa atgggccagc aacgccttat tgagcgcctt caccgccagc ggattggcaa
     5341 agtctacgct ttgctccccg gcgggtgtaa gcgtgagga
//
LOCUS       DAEVZA010000225         5370 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEVZA010000225 DAEVZA010000000
VERSION     DAEVZA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.225"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(86..940)
                     /gene="kdsA"
                     /locus_tag="LAF92_004878"
     CDS             complement(86..940)
                     /gene="kdsA"
                     /locus_tag="LAF92_004878"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBI9793765.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(976..1785)
                     /locus_tag="LAF92_004879"
     CDS             complement(976..1785)
                     /locus_tag="LAF92_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBI9793766.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(1789..2181)
                     /locus_tag="LAF92_004880"
     CDS             complement(1789..2181)
                     /locus_tag="LAF92_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBI9793767.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(2178..3011)
                     /gene="prmC"
                     /locus_tag="LAF92_004881"
     CDS             complement(2178..3011)
                     /gene="prmC"
                     /locus_tag="LAF92_004881"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBI9793768.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(3011..4093)
                     /gene="prfA"
                     /locus_tag="LAF92_004882"
     CDS             complement(3011..4093)
                     /gene="prfA"
                     /locus_tag="LAF92_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBI9793769.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(4135..>5370)
                     /gene="hemA"
                     /locus_tag="LAF92_004883"
     CDS             complement(4135..>5370)
                     /gene="hemA"
                     /locus_tag="LAF92_004883"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBI9793770.1"
                     /translation="INHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGGVVLSTCN
                     RTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMRVASGLDS
                     LVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGASAVSVAF
                     AACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRERAQILADE
                     VGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLLVDIAVPR
                     DVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMAWLRAQSA
                     SETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAPTKSLQQA
                     ARDGDNERLNILRDSLGLE"
BASE COUNT         1302 a         1473 c         1291 g         1304 t
ORIGIN      
        1 cagcctgcgg ccttttttgt atttggcacc catgaaaaaa gtcttaacgc agaacgctaa
       61 gactttattt ttcaagcaaa aaagattact tgctggtatc cagttcttcg aaacctttca
      121 ccagatcatc aatcgctttc atctgcttga ggaacggttc cagtttagcc agcggcagcg
      181 cggatggacc atcacatttc gcatgttccg gatccggatg cgcttcaata aacagccccg
      241 ccagacctac cgccataccg gctcgtgcca gctcagccac ctgagcacga cgaccaccgg
      301 aagctgcgcc aaacggatcg cggcattgca gtgcgtgggt cacgtcgaaa atcaccggcg
      361 agttaccaga cactttcttc ataatgctga agcccagcat atcgacaacc aggttgtcat
      421 agccgaagtt agcaccacga tcgcaaagaa tcactttttc gttgccacct tctttgaatt
      481 tatcaacgat attacccatc tgtcccgggc tgacaaactg tggtttcttg acgttaatta
      541 ccgcaccggt tttcgccatg gcttcaacca ggtcagtctg gcgagcaaga aacgccggca
      601 actgaatcac atccacgaca tcagcaacgg gctgtgcctg acttggttcg tgaacgtcgg
      661 tgataatttt cacgccaaaa gtctgcttca actcctggaa gattttcatc ccttcttcca
      721 ggcccggtcc acgataagag tggatggagg agcggttggc tttgtcaaaa gaggctttga
      781 acacgtaagg gatacccagt ttctgggtca cagttacgta gtgctcgcaa atgcgcatcg
      841 ccagatcgcg agattccagc acgttcatac cgccaaacag tacgaacggc aggtcatttg
      901 ctacgttgat gtcgccaatg ctaaccactt tttgtttcat aggatcgcct taatcttgag
      961 taaaatgtcg attaattaat gcagcacaat atgtttatgc gcgatgttat ttatttgcgc
     1021 acggatcatt tcgctgatcg ggtcttccgg acactgttca acgaaataac ttaaatcgtt
     1081 caacgcaacg tgttcgcaat ccagttgcgc ataaatcaac ccgcgatcgc gaatttcata
     1141 gggatcttca gggttgaatt gtaataaagc ttcgctggtg cgtaacgcca gctccatctg
     1201 attttcttcc atcaacgagg ctttgagtgt atccagcaat ttgcggatta cctcaatgtt
     1261 atcagcttca tcaaggtctt cataaaacag ttccgccgac gggctgatat ttccctttaa
     1321 ccatacgtcc agcatatgtt cgcttaacga ttcaccgtta aaaggattaa tcagccaaat
     1381 ttcgccatcc ggacattcaa tgcgcaatat cagctgcgta gggaaaatca ccggcagcag
     1441 cggcaaatcg agacgattcg cgacccataa taaaaccgca cctaatgata ccgcactgcc
     1501 ctgtcgattc tttaacacct ggtccagcca taatgcatcg gaaagacgat aaacgccgcg
     1561 tgaggcttta aatccccagt cgccgtaaaa cagcgcgatc agtttttcca gctgctcttc
     1621 taaaggcaga agctggctga tttcttcctt cgccagacta acgagacgct ccagttcgtc
     1681 gtagacatct tgcgagggaa aatcgcggcg gattgcttcg caagccagga tcatgccttc
     1741 gcacaatggc gctttattaa attcgaaatc agctaacgat ctcatgactt accccagtaa
     1801 cggtactttt gtggtggcga gtttaatgat gatgtacagc accaccaacg ccagcgggaa
     1861 ggcaataatg cgcgcctgct gactgtgcat acgacgataa tcgagtgcaa taaaacccaa
     1921 aacgatataa atgataactc caaacagctt ttcagtcagc cactgtgcct gcccggaaaa
     1981 tggcaggatg tgcgctttag ccatcaacgc aatgccgctt aacaataaca ccgtatcgac
     2041 aaccggcggc acaatgcgcg tccagcgagc aaatgcctgt ggatgcttct gataacgtag
     2101 ccagaaacgt aaggttaata gcccaacaga aagcgcgata ctaataagat gaacactaag
     2161 cagtgtagaa aaacttgtca ttgataatag cggccgagcg ttacgcgctc gttatcacca
     2221 tagtcacggc aggtttcgac gtcatgatac cccgcgagga taaatgcttg tcgcaccgct
     2281 tcgccctgct gccagccatg ttccagaagc agaaagccgc cggataccag cgcgttacgc
     2341 gactgttcga tgatatgcac gatgtctgcc attccactgt ctgccgcaac cagcgcagtg
     2401 agcggctcaa agcggacatc gccttgttga agatgtgggt cctgctcgtc aatatacggc
     2461 ggattgctga caatcatcgc aaactgctgc ccggctagcg cgctaaacca gtcgctttgc
     2521 agaatgtgga tatttttgat cgccagatgc tgggcattac gttgtgccag ggagacagca
     2581 tcaggcatac gatctacagc gataatttcg cagtccgggc gctcgctagc cagcgcaagc
     2641 gcaatcgccc cggtacccgt cccgagatcg agaatacggc aaggttgttc aggcaaccgc
     2701 gccagtgcct gctccaccag acactccgta tccgggcgcg gaattaaggt cgctggcgaa
     2761 acaaataacg gcaacgacca gaattctcgc accccggtta aatgagcaat gggttcacca
     2821 tcgcgacgac gtgtcagtag cgcatcaagt tgctgacatt gttcgtcagt cagctgcgtt
     2881 tcaccaaagg ccagaataaa agtccgcccc ctgccagtga catactccaa taagatttca
     2941 gcgtcacggc gtgggctttc gctcgcctga agttggctta ttgcttcacg taaccagtat
     3001 tgatattcca ttattcctgc tcggacaacg ccgccagttg gtcggcctga tgttcctgga
     3061 taatcggttc aatcagcata tccagcttac cttccatcac ttcatccaga cggtagagtg
     3121 tcaggttgat gcggtgatcg gtaacgcgcc cctgcgggaa gttgtaagta cggttacggt
     3181 cgctgcgatc gccactcccc agcaggttac gacgggtaga cgcttcggcc tgttgccgtt
     3241 ttgccatttc tgcagcgtgg atacgagcac caagtacaga aagtgccttc gctttgtttt
     3301 tatgttgtga acgttcatcc tgacattcaa caacaatccc ggtcggcaag tgagtaatac
     3361 gaattgccga atcggtggtg ttaacgtgct gaccacccgc ccctgacgag cggaaagtat
     3421 caatgcgtaa atctgctggg ttgatgtccg gcagttctgc gtcaggcagt tctggcatta
     3481 ccgcaacggt acaagcagaa gtatgaatac gaccctgcga ttccgtagca ggaacacgct
     3541 gcacgcgatg accgccagat tcaaatttca gacgaccata cacaccatca ccgctaattt
     3601 tggcgatgat ctctttataa ccaccatgtt caccctcgct ggcgctcatg atttctaccc
     3661 gccagcggcg ggcttcggcg taacggctgt acatacggaa cagatcgcct gcgaacagcg
     3721 ccgcttcgtc gccgccagtt ccggctcgga cttcgaggaa ggcgttacgt tcgtcatcag
     3781 gatcttttgg cagtaacaga acctgtaatt gctgttccag ttgctcgctt ttttctttag
     3841 cttcgcgcag ttcatcctgc gccatctcac gcatttcagg atcatcgagc atcatctgtg
     3901 cggtttcgat atcttcctga acctgttgcc agtcggtaaa acagcgcgaa acatcactta
     3961 actgcgcata ttcgcgtgat aatgcgcgaa aacgttcctg gtcggcgata gtttgcgcgt
     4021 cacccagcaa cgcctgaact tcttcatggc gttcatgcag ggcttccagt ttggcaacga
     4081 tagaaggctt cataggcgta aatgcaccct gtaaaaaaag aaaatgatgt actgctactc
     4141 cagcccgagg ctgtcgcgca gaatattcag gcgttcgtta tccccgtcac gggcggcctg
     4201 ttgaagtgat ttcgttggcg catggatcaa gcggttagtc agtttccatg ccagatcctg
     4261 cataatggct tgcgcgtcgc cgccctgctc aagggccgct aacgctttgg cggttaactc
     4321 atcgcgaacg tgctctgcct ggctgcgata ctcgcgaatg gtttcgctgg cgctttgtgc
     4381 tcgcagccac gccataaatt cgctggtttc ctgagcgaca atagtttccg cctcaaccgc
     4441 tgcggcttta cgctgcgcca ggttgtgcga aatgatgctt tgcagatcgt ccacactata
     4501 aagataagca ttcgccagtt tgccaacttc cggctcaaca tcgcgcggaa cggcaatatc
     4561 caccaacagc attggttggt tgcgacggct ttttaatgcg cgctccacca tgcctttccc
     4621 gataatcggt aacgggctgg cggtggaact gatgatgata tcggcttcgc gcagacgttc
     4681 gtcgatctca ctcagggcaa tcacttccgc gccaacttca tctgccagaa tttgggcacg
     4741 ttcgcgagtg cggttggcga taatcatctt ctgtacttta tgttcgcgca gatgacgcgc
     4801 taccagctcg atggtttcgc ccgcgcctac cagcaacact gtgaccgtag agagcgattc
     4861 aaagatctgc cgcgccagcg tacaagccgc aaaagcgaca gacacagcac tggcaccgat
     4921 atctgtttca gtgcgaacgc gtttcgcgac agagaaagat ttctggaaca tgcgttccag
     4981 ttcgctggcc ttcatatgac ctttttgcga atcggcaaac gcttttttaa cctgaccgag
     5041 gatctgcggc tccccaagaa ccagtgaatc caggccgctg gcaacacgca ttaaatggct
     5101 aaccgcgtcg ttatcctgat gccagtagag gcttttacgc agatcttctt cattaagatt
     5161 gtgataatcg caaagccagc ggattaacgc ctcttgcagg ttatcctgct cttcaacgct
     5221 aagataaagt tccgtgcggt tgcacgtcga cagcaccacg ccgccctgca ccatcggctg
     5281 cgcaagcagg ctgtcaagcg cctgatcgag cttatccggc gaaaacgata cacgttctcg
     5341 cagcgataca ggtgccgttt tatggttgat
//
LOCUS       DAEVZA010000226         5219 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEVZA010000226 DAEVZA010000000
VERSION     DAEVZA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.226"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            192..392
                     /locus_tag="LAF92_004884"
     CDS             192..392
                     /locus_tag="LAF92_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793771.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVSLEVRGFRKEAAEAWLLAAR
                     VAPRTDWQQFARQRAERCQRRR"
     gene            778..1377
                     /locus_tag="LAF92_004885"
     CDS             778..1377
                     /locus_tag="LAF92_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793772.1"
                     /translation="MATGKSEKQQVIAFIDYENCSNLTEISLNIYTELIIFAGAKQNN
                     VALPVNAFPEAVKITLRHVSEVSRNNVDFHLVLELGQCVCHYGPEKEYHIVSADKGYD
                     GIVRTLQARGIRCRRVSPDKASSVKMAAPDMDIVIRWANKIQQAAREVRNMPVTEKTF
                     NNYLKSQMRGEWRDVLTRGIRDELVRRGVMKIQGNKIIW"
     gene            1541..1771
                     /locus_tag="LAF92_004886"
     CDS             1541..1771
                     /locus_tag="LAF92_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793773.1"
                     /translation="MTVAVRGKQEFIIAELDSEIRKIRLKLTDSYEEGVRLSSGTFTL
                     PARFCREILPDDVRSITIILEKSDDEWWYGSY"
     gene            2076..2225
                     /locus_tag="LAF92_004887"
     CDS             2076..2225
                     /locus_tag="LAF92_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI9793774.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            2509..2757
                     /gene="repA"
                     /locus_tag="LAF92_004888"
     CDS             2509..2757
                     /gene="repA"
                     /locus_tag="LAF92_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBI9793775.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQEMCDAEGVTQAEMIAELIKQKSAFS"
     gene            3002..3076
                     /gene="tap"
                     /locus_tag="LAF92_004889"
     CDS             3002..3076
                     /gene="tap"
                     /locus_tag="LAF92_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBI9793776.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGWCD"
     gene            3069..3926
                     /gene="repA"
                     /locus_tag="LAF92_004890"
     CDS             3069..3926
                     /gene="repA"
                     /locus_tag="LAF92_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBI9793777.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGVLSITRATRALTSLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEVAVASARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIITLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            4627..4767
                     /locus_tag="LAF92_004891"
     CDS             4627..4767
                     /locus_tag="LAF92_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793778.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
     gene            4865..>5219
                     /locus_tag="LAF92_004892"
     CDS             4865..>5219
                     /locus_tag="LAF92_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBI9793779.1"
                     /translation="MIQTRNQYLQFMLVMLVMLAAWGISWGARFVMEQAVLLYGSGKN
                     YLFFSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKA
                     LALPAEPFMTKLFADK"
BASE COUNT         1397 a         1218 c         1368 g         1236 t
ORIGIN      
        1 aaaatccgcc gccagaaccg gataaaggca gaacttcagg ccgtgcttga tgagaaataa
       61 aaaaaagcct ccatcagaga ggaggcaggg aaataaggct taaaaggaaa taacttccgt
      121 acccgaaagg aagccaattc agtagattat ttaacaccat aatactgacg ggttaaagag
      181 gagtgataaa gatgaatgga ttcagaaaca gttcacggaa cggtcaggtc tggcgctggc
      241 agcgtgccgg aagccgtgcc gtcagcctgg aggtcagggg attccggaag gaggccgctg
      301 aagcgtggct cctggctgcc cgtgttgctc cccgtacaga ctggcagcag tttgcacggc
      361 agagagccga acgctgtcag cgacggagat aatgaaaaaa aacggcaaaa aaaagacttc
      421 ccctgagcaa aggaggccaa aaatcattca tagcgtatta ctgacattga atgaatgctc
      481 tgtacatcaa ccctgtgaag ctaccaccgg ttaatgccgg gaggttgcgg gaagaaagat
      541 aatacacagt cattacgtgg ttaatatctg tcagaatgtt ttttgtgacc ataaactgtc
      601 agatggataa aaaagaccgt catgtgacgg tcagtgttgt cctgtcaggg acaaatgcat
      661 tcagtaagga gagcaaaata ataacatcac gttgcccggc tgttaatatc aggcgatgtt
      721 aagtgttatt tatgactctt ttatctggtt gtgaagtctg ttaatcagga agagaaaatg
      781 gcgaccggaa aatcagagaa gcaacaggtg attgccttta tcgattacga gaactgcagt
      841 aacctgacag aaatttcctt aaatatatat acggagttaa ttatattcgc gggtgcgaaa
      901 cagaataatg tggcactgcc agtaaacgct ttccctgaag cagtaaaaat cacactccgg
      961 catgtcagtg aagtctcccg gaacaatgtt gattttcatc tggtgctgga gctggggcag
     1021 tgtgtatgcc actacgggcc ggagaaggaa taccatattg tttccgctga caagggatat
     1081 gacggtattg tccggacact gcaggcgcgc gggattcgct gccgtcgcgt ctccccggat
     1141 aaggcgtcat cagtaaagat ggctgcaccc gacatggata tcgttatccg gtgggcaaac
     1201 aaaattcaac aggcagcgcg ggaggttcgt aatatgccgg taacagaaaa gacatttaat
     1261 aattatttaa aatcacagat gcgcggtgaa tggcgtgatg tcctgaccag aggtatccgg
     1321 gatgaactgg tccgtcgcgg ggtaatgaaa attcagggga ataaaatcat ctggtaagac
     1381 tgccagaata aaatgatatt tattttattt ataaaaacag aaaggtcgaa aatcaataat
     1441 ggcatttatt tccatgacaa gacagaaagc gccgtacaga tatgcaagca agacccggtt
     1501 atcgataaga cgaacagact caggctctgc cgggtgttat gtgacggttg ccgttcgggg
     1561 gaagcaggaa tttattattg ctgaacttga ttcagaaatc cggaaaatca ggctgaaact
     1621 gacggactcc tatgaagaag gggtaaggct gtcttcgggc acgtttacgc ttccggccag
     1681 attctgccgg gaaatactgc cggacgacgt caggagtatc accattattc tggaaaaaag
     1741 cgacgatgag tggtggtacg ggagttactg atatgtggca gaactccgga cactgacgcc
     1801 ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa gcaagttttc cccgctcccg
     1861 gcgtttctgc aataaaaagc aggggcatgg aggtcgtcgt aaccagacag gaatgtggta
     1921 ttatcgcggc gggtgtctga gcctttctgg ttcaggcaag acgcaggtac cagaaatgcg
     1981 aagaccccac tcgttaatcc attaactcgt gaggtctgca tgaagtactt agcactactg
     2041 attgtagcct cttctttgca gagaggtcaa agtttatgac gaaatatgcc cttatcgggt
     2101 tgctcgccgt gtgtgccacg gtattgtgtt tttcactgat attcagggaa cggttatgtg
     2161 aactgaatat tcacagggga aatacagtgg tgcaggtaac tctggcctac gaagcacgga
     2221 agtaagctgc cgggcgggga cggaagtccc cgctttccgg aagtgtgagg tatttcaggg
     2281 gcagacaccc gacatgccag aaacagccgg tcccgcccgg ggccggcatc caggttaagg
     2341 catttcctgt ttttcagtca tttcatcatc aaaatcacat taaacggtcg taatcagaca
     2401 tgatttgtgc gccaacacag atcatcatca caactttcaa gtcgctgatt tcaaaaaact
     2461 gtagtatcct ctgcgaaacg atccctgttt gagaattgag gaggcgagat gtcgcagaca
     2521 gaaaatgcag tgacttcctc atcaggtaca aagcgagcat accgaaaagg taatcctctg
     2581 acgcttgcag aacgtcagca ggcatctctg gcaagaaaac gtgccacaca taaagaactc
     2641 agagtgttca ttcctgctgc gttaaaagcg caattacagg agatgtgtga cgctgaaggt
     2701 gttactcagg ctgagatgat tgctgaactg ataaagcaga agagtgcttt cagctaacta
     2761 aaatgtagcg acatcacatt cttgcctgtt tgtggtgtgc gtgatagatt gctgatcgtt
     2821 taaggaattt tgtggctggc cacgccataa ggtggcaggg aactggttct gatgtggatg
     2881 tacaggagcc agaaaagcga aaaccccgat aatcttcttt aactttggcg agtcagaaag
     2941 attaccgggg ctaacaagaa actgcataga agctgttgct ctatgcgggg agtatagtta
     3001 tatgcccgga aaagttcaag acttctttct gtgctcactc cttctgtgca acataagtgc
     3061 aggatggtgt gactgatctt caacaaactt attaccgcca ggtaaagaac ccgaatccgg
     3121 tgtttacacc ccgtgaaggt gccggaacgc tgaagttttg cgaaaaactg atggaaaagg
     3181 cggtaggctt cacctcccgt tttgatttcg ccattcatgt ggcacatgcc cgttcgcgtg
     3241 gtctgcgtcg gcgcatgcca ccggtactgc gtcgtcgggc tattgatgcg ctgctgcagg
     3301 ggctctgttt tcactatgat ccgctggcca accgtgtcca gtgctccatc accacgctgg
     3361 ccattgagtg tggactggcg acggagtctg atgccggagt actctccatc acccgtgcca
     3421 cccgtgccct gacgtccctg gctgagctgg gtctgattac ctaccagacg gaatatgacc
     3481 cgcttattgg ttgcaacatt ccgaccgata tcacgttcac accggcgctg tttgccgctc
     3541 tcgatgtgtc tgaggtggcg gtggcttccg cacgccgcag ccgtgtggaa tgggaaaaca
     3601 gacaacgcaa aaagcagggg ctggataccc tgggcatgga tgaactgata gcgaaagcct
     3661 ggcgttttgt tcgtgagcgt ttccgcagtt accagacaga gcttaagtcc cggggaataa
     3721 agcgtgcccg tgcgcgtcgt gatgcggaca gggaacgtca ggatattatc accctggtga
     3781 aacggcagct gacgcgcgaa atcgcggaag ggcgcttcac tgccagtcgt gaggcggtaa
     3841 aacgtgaagt ggagcgtcgt gtgaaggagc gcatgattct gtcacgtaac cgcaattaca
     3901 gccggctggc cacagcttcc ccctgaaagt gacctcttct gaataatccg gcccgaaccg
     3961 gaggcattcg cacgcctgaa gcctgccagc gaacaaaaaa tcagcaccac atacaaaaaa
     4021 caacctcact atccaccttc tggagcatcc ggttccccct gtttttaata caaaatgcgc
     4081 ctcagcgacg gggaattttg cttatccaca tttaactgca agggacttcc ccataaggtt
     4141 acaaccgttc atgtcataaa gcgccagccg ccagtcttac agggtgcaat gtatctttta
     4201 aacacctgtt tatatctcct ttaaactact taattacatt catttaaaaa gaaaacctat
     4261 tcactgcctg tcctgtggac agacagatat gcacctccca ccgcaagcgg cgggccccga
     4321 ccggagccac tttagttaca acacacaaaa acaacctcca gaaaaacccc ggtccagcgc
     4381 agaaccgaaa ccacaaagcc cctccctcat aactgaaaag cggccccgcc ccggcccaaa
     4441 gggccggaac agagtcgctt ttaattatga atgttgtaac tacatcttca tcgctgtcag
     4501 tcttctcgct ggaagttctc agtacacgct cgtaagcggc cctcacggcc cgctaacgcg
     4561 gagatacgcc ccgacttcgg gtaaaccctc gtcgggacca ctccgaccgc gcacagaagc
     4621 tctctcatgg ctgaaagcgg gtatggtctg gcagggctgg ggatgggtaa ggtgaaatct
     4681 atcaatcagt accggcttac gccgggcttc ggcggtttta ctcctgtatc atatgaaaca
     4741 acagagtgcc gccttccatg ccgctgatgc ggcatatcct ggtaacgata tctgaattgt
     4801 tatacatgtg tatatacgtg gtaatgacaa aaataggaca agttaaaaat ttacaggcga
     4861 tgcaatgatt caaacacgta atcaatatct gcagtttatg ctggttatgc tggttatgct
     4921 ggctgcatgg ggcattagtt ggggagccag atttgtcatg gagcaggccg ttctgcttta
     4981 tggatcagga aaaaactatt tgttcttcag tcatggtact gttctgatgt acctgctgtg
     5041 tgttttcctg gtataccgcc gttggatagc tccgctaccg gtcgttggtc agctgcgcaa
     5101 cgttggcgta ccgtggctgg tcggtgcgat ggccgtggtg tatgtcggtg tatttctgct
     5161 cggtaaggcg ctggctctgc ctgctgagcc atttatgacg aaactttttg ccgataagt
//
LOCUS       DAEVZA010000227         5202 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEVZA010000227 DAEVZA010000000
VERSION     DAEVZA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.227"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(134..922)
                     /locus_tag="LAF92_004893"
     CDS             complement(134..922)
                     /locus_tag="LAF92_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HBI9793780.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(1053..1526)
                     /locus_tag="LAF92_004894"
     CDS             complement(1053..1526)
                     /locus_tag="LAF92_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HBI9793781.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            1684..2697
                     /gene="intI1"
                     /locus_tag="LAF92_004895"
     CDS             1684..2697
                     /gene="intI1"
                     /locus_tag="LAF92_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBI9793782.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2900..3250
                     /locus_tag="LAF92_004896"
     CDS             2900..3250
                     /locus_tag="LAF92_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBI9793783.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3426..3986
                     /locus_tag="LAF92_004897"
     CDS             3426..3986
                     /locus_tag="LAF92_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI9793784.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            3990..>5202
                     /locus_tag="LAF92_004898"
     CDS             3990..>5202
                     /locus_tag="LAF92_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HBI9793785.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRA"
BASE COUNT         1091 a         1552 c         1525 g         1034 t
ORIGIN      
        1 tttggctgtg agcaattatg tgcttagtgc atctaacgca tagttgagcg gcgggcgcag
       61 cccgtccgct tgaacgccga gttaggcatc agatgccctc ggcgcgggtc gatgcacttt
      121 tcgcacatgc cgctcaacgc aagattctct caatcgttgc tttggcatat cgaacgaacg
      181 cggccgtctc ttcgacgcgc attgctaggt cgtcgtcctc gctacccagg tacgccgcgc
      241 gtgccttgca gatgaggggc cgatgctcgg caggcaaacg ctccgatacc catgcggcag
      301 caacgtcctt aggagcaatg agaccagttg aagcgctgta ccaaatgcga gcaagagcaa
      361 gaacgacgtt ccgctcgtca cccttccaat ccgactctgc attccactgg gcaatagtgt
      421 cgaaaagcgc cttggagaaa tgctccttcg gcaccggctc gaaaaacgtg gctgcggatg
      481 ggcctagaag cgcaaggctg tgttgcctcg ccttggtcag caaaatcgca agatcgtgat
      541 ccagaacggc aggctcgaac gttccggaaa ggatgtcgtg gcggagccac tcaccgaact
      601 gaagctcacg ccgcgccgga tagcgccaag gcactacttc gcttcgagcg acaacagtta
      661 gctccagcgg tcgccatgtt ccgccatcgc ctggcggtga tgagactttc agcaaatcga
      721 gcattagcgc ctgccggagc gaatcgttag gtgcggcgct gacggtcacg agcaagtcta
      781 tgtcgctgtc cggcttcagc cctccatcga tcgcagatcc gaacaggtgg attgtgtcca
      841 gtgtcgcagc cagatggcgc tcgatcaccg cgcgagcgtg ggacagctgc ttgaaaactt
      901 gtgcagggaa aaattcaccc atgatgccta acgttaagtt cagcggcagc ttttaagttg
      961 cggctttgtg gaatactttt gcgcagcaaa accacaaaga cgcgacttaa aagctgtcca
     1021 aggagcgaag cgactggtgc tgcaacgcat tgttagcctt ttttccaaat ctggtatgta
     1081 taatttatat tagacataaa aaactgttca aaaaccaaat tgaaattctc aggcattata
     1141 gggaatttga tatcaccttc gacttcaacg tgaacagtag acaaatgaat tatatctgct
     1201 ttttcaataa ggctattata gatttgaccc ccgccagaga catatacatg atctgtaact
     1261 tttgatagct ctttcaaagc attttctatt gaaggaaaaa ctaggacgtt ttcatttgag
     1321 cttgaaattc cgttctttga cactactgca tatttgcgat ttggaagaac acccatagag
     1381 tcaaatgttt ttcttccgac aaggagccat tgattatatg tgagcgcttt aaagagtagt
     1441 tgctcacctt ttactgacca cgggatatca ggaccactac cgattacgcc attttctgac
     1501 actgcagaaa tcaatgatat tttcaattta actcccttaa tggctaactt tgttttaggg
     1561 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
     1621 taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa
     1681 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
     1741 ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtccac
     1801 tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc
     1861 gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat
     1921 cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc
     1981 tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg
     2041 gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccagctt
     2101 ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat
     2161 ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg
     2221 ttacccgaga gcttggcacc cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg
     2281 ctgaaggacc aggccgaggg ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag
     2341 tatccgcgcg ccgggcattc ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg
     2401 accgatccac ggagcggtgt cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc
     2461 gccttcaaac gtgccgtaga acaagcaggc atcacgaagc ccgccacacc gcacaccctc
     2521 cgccactcgt tcgcgacggc cttgctccgc agcggttacg acattcgaac cgtgcaggat
     2581 ctgctcggcc attccgacgt ctctacgacg atgatttaca cgcatgtgct gaaagttggc
     2641 ggtgccggag tgcgctcacc gcttgatgcg ctgccgcccc tcactagtga gaggtagggc
     2701 agcgcaagtc aatcctggcg gattcactac ccctgcgcga aggccatcgg tgccgcatcg
     2761 aacggccggt tgcggaaagt cctccctgcg tccgctgatg gccggcagca gcccgtcgtt
     2821 gcctgatgga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     2881 ccgtcttctg aaaacgacaa tggaggtggt agccgagggt gtggaaacac ccgactgcct
     2941 tgcgtggttg cggcaggcgg gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat
     3001 gccggcggcg gccttcgtcg gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa
     3061 tgaaccgagc accagttgct gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac
     3121 gccggaaggg gccgagtacg tggagcattt ctgcgggctg gagtgctatc agcgcttcca
     3181 ggcgcgggcc agcactgcga ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc
     3241 gtcaggttga ggcataccct aacttgatgt caggcagggc cgcgccgctt cgtcagaata
     3301 gagtctgctt tcccattttt tgacacatgc ccgcgaaggt tatagatttc agcctgacag
     3361 aaatgggctt tgaggcacaa cggaacagaa agtgcactta agccgccttc aaccaaggag
     3421 acatcgtgca ggggcaccgc atcggctacg tccgggtcag cagctttgac cagaacccgg
     3481 aacgccagct ggaacaaacc caggtgagca aggtgttcac cgacaaggca tcgggcaagg
     3541 acacccagcg cccccagctc gaagcgctgc tgagcttcgt ccgcgaaggc gatacagtgg
     3601 tggtgcacag catggatcgg ctggcccgca acctcgatga cctgcgtcgc ttggtacaga
     3661 agctgactca gcgcggcgtg cgcatcgagt tcctgaagga gggcctggtg ttcactggcg
     3721 aggactcgcc gatggccaac ctgatgctgt cggtgatggg ggccttcgct gagttcgagc
     3781 gcgccctgat ccgcgagcgg cagcgtgagg gcatcgcctt ggccaagcag cgtggcgcgt
     3841 accggggccg caagaaagcc ctgtccgatg agcaggctgc taccctgcgg cagcgagcga
     3901 cggccggcga gcccaaggcg cagcttgccc gcgagttcaa catcagccgg gaaaccctct
     3961 accagtacct ccgcacggac gactgacaca tgccgcgtcg cttgatcctc tcggccacgg
     4021 agcgggacac cctgcttgcg ctgccggaaa gccaggatga cctgatccgc tactacacct
     4081 tcaacgactc cgacctgtcg ctgatccgcc agcgacgcgg cgacgccaac cgcctcggct
     4141 tcgccgtgca gctctgcctg ctgcgctacc ccggttacgc gctgggaacc gacagcgagc
     4201 tgcccgagcc ggtcatcctg tgggtggcga agcaagtcca ggccgagccg gcgagctggg
     4261 caaagtacgg cgagcgcgac gtgacccgtc gcgagcatgc ccaggaactg cgcacctacc
     4321 tgcaactggc cccgttcggc ctgtccgact tccgcgccct ggtgcgcgag ctaaccgagc
     4381 tggcccagca gaccgacaaa ggcttgctgc tggccggtca ggccctggag agcctacggc
     4441 agaaacgacg catcctgccg gcgctgagcg tgattgaccg ggcctgctcg gaagccattg
     4501 cgcgagccaa tcggcgggtc taccgcgccc tggtcgaacc actcacggac tcgcatcggg
     4561 ccaagctgga cgagctgttg aagctcaagg ccggcagcag catcacctgg ttgacctggc
     4621 tgcgccaggc accgctgaaa cccaactctc ggcacatgct ggaacacatc gagcggctga
     4681 agacatttca gttggtggac ttgcccgaag gcctgggccg gcacatccac cagaaccgcc
     4741 tgctcaagct ggcccgcgag ggtgggcaga tgacgcccaa agacctcggt aagttcgagc
     4801 cgcagcgccg ctacgcgacc ctggccgccg tggtgctgga gagcaccgcg accgtgatcg
     4861 atgagctggt cgatctgcat gaccgcatcc tggtcaagct gttcagcggc gcgaagcaca
     4921 agcatcagca gcagttccag aagcagggca aggcgatcaa cgacaaggtg cgcctgtact
     4981 ccaggatcgg ccaggcgctg ctggaagcga aggaaagcgg cagcgacccc tatgccgcca
     5041 tcgaggcggt gattccctgg gacgagttca ccgagagcgt cagcgaggcc gagctgctgg
     5101 cccggccgga aggcttcgac cacctgcacc tggtcggcga gaacttcgcc accctgcgcc
     5161 gttacacgcc ggccttgctg gaggtgctgg aactgcgcgc cg
//
LOCUS       DAEVZA010000228         5167 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEVZA010000228 DAEVZA010000000
VERSION     DAEVZA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.228"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1310..1519
                     /locus_tag="LAF92_004899"
     CDS             1310..1519
                     /locus_tag="LAF92_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793786.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(1585..2118)
                     /locus_tag="LAF92_004900"
     CDS             complement(1585..2118)
                     /locus_tag="LAF92_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793787.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2121..3047)
                     /locus_tag="LAF92_004901"
     CDS             complement(2121..3047)
                     /locus_tag="LAF92_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBI9793788.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            3224..3622
                     /locus_tag="LAF92_004902"
     CDS             3224..3622
                     /locus_tag="LAF92_004902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793789.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(3826..4740)
                     /locus_tag="LAF92_004903"
     CDS             complement(3826..4740)
                     /locus_tag="LAF92_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI9793790.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
BASE COUNT         1435 a         1352 c         1105 g         1275 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag
      721 cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc agtactatcc
      781 atacagctaa ttacatataa atactattaa accatctgga cattaaaaat aaactcttat
      841 aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat
      901 cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag
      961 ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag
     1021 aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac
     1081 ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact
     1141 tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc
     1201 tccttacata tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa
     1261 tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca tggcaaaatt
     1321 aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat caagccaggc
     1381 atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag ctcttactga
     1441 tgagcacgta ccgttatata tacgaaggct ggaatactat cgtctgcttt atcgaccaaa
     1501 gcagacagaa tcatattgaa aaaaacggtg aacggttcgc gaaatccttt cgcgggtcgt
     1561 gaggcgttgg acaagcgaag cgcgtcagtt tttccggctc ttccccgtga caggtttctc
     1621 caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt
     1681 cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac
     1741 tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt cctgcacgat
     1801 attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct tcatgtttgc
     1861 gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca gtcctgccac
     1921 gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca caccgtcaga
     1981 aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag caacagcttt
     2041 ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg tgagtaattt
     2101 ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc
     2161 cgttcaggtg cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa gccatgctcc
     2221 cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg agtgtccagc
     2281 cattccctga tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga atagtccggt
     2341 tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc ggcagcttca
     2401 aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt ccggcagtca
     2461 atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac ctcccagcgt
     2521 tttcgcaatt cccgcaactg ctctttttgc tgagtgagag tcaggcttcc ggaatccttt
     2581 ttcgcacctc cccgctcggg atacctggcg ttatagcgcc ggaaatactg ttccggtgta
     2641 cgttcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg ctgttcgccg
     2701 ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg ttcgccagct
     2761 tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag ttcacggggt
     2821 aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg gtcagccgcc
     2881 tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg tgcggaatat
     2941 tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc gtggtttgca
     3001 gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt ttcagccctc
     3061 tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct tccttctaat
     3121 tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga tatactgcac
     3181 gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca tcagcaacat
     3241 cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga ccgcacgaca
     3301 gagacagatt attgacctga tcgacaacga agccggactc acagaacagg aacgcaaact
     3361 tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga gcgaacgaaa
     3421 acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca atcaccggct
     3481 ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa caggcgagct
     3541 atgctataaa aaagacagga tattacaagc acttaaggaa cttaagtacg atctggagac
     3601 atccccaaac ccagacgcgt aagtgcctcc atccgtgttc cgattccctg gtgcttcctc
     3661 gctcattcgc tcgctacgct cggtcatgag actgcggcga gcactaccac cacaatcacg
     3721 cggaaagccg cgccattgca gggctgaacg gggaaaaacg gcaaaaaacc tgctgctcaa
     3781 aaagacgcca gaaaggcgct gttatctgat ataggcttat ttggtctact ctacgagacc
     3841 tcgttttttc agttggtaat accaggcgcg actgatccct aatgcctccc acggtttcag
     3901 cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta gaaccgccct tgcgaccgcg
     3961 tgcagcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag tttccggcgt
     4021 gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt cagccagtga
     4081 aagcggaacg ggaagcgatg cattgtacat ttcgacacgc tggatcaccg cttcaagcca
     4141 ctgtgagaac gctggccagc cctgacgaat cgccctgtaa gcccatttgc gcgtcatttc
     4201 gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacgac gctctgaggc
     4261 gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctcgc gccactccat
     4321 caccagccat tccaggtgga acggattttt gcaaatcagg ccgctgtagt tcacatccgc
     4381 acaaagtttc tcacacagcg aacgttcaac cgctgcggca tatttcagcg ccttaaccga
     4441 ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag agcaggtgag cgtgtccgtt
     4501 cacaggattt ttaacggtga tgttcggcgc gggagcgttc cggtcgctcc agtcaatcgc
     4561 agccccctcg cggtcaacgt caaaaaccag ccagaactgc gcatgaggct ggttctgctg
     4621 tatgtaccgc gcaagaagcg cacgcccttt gccggagatg cgaagaccaa aagcgagatc
     4681 atccgtgtga tagggacgat ggggtaaatt ttcgtcgaag tattgaagcg cggcgctcat
     4741 gtgttacgat tctctccggg ttatgcgacc tacacgccct ctgtatttca tgttgaatct
     4801 tgccggatct ggacatgatt tcaggggcgt ttgtttttca gtcgcttaca ctacacccaa
     4861 cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt taccataact cccgttgtac
     4921 gcccccctta aaacgccctc agtcgctttc tggcgcaaaa aacgcaaaaa gacaagaaaa
     4981 caccaggcca aaccagaaaa acgcgtcaga atcgttttca gggcgttgca taactatgca
     5041 cgaaagcgat gtgcgatcgg tttcgtgcgt tctgaacacc gaacggctca aaacgacaag
     5101 ttttctcccc tggtgcgtgg cgctctgacg gggtttcctg tttccaaact tcacatctcg
     5161 ccaggac
//
LOCUS       DAEVZA010000229         5032 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEVZA010000229 DAEVZA010000000
VERSION     DAEVZA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.229"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="LAF92_004904"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="LAF92_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBI9793791.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="LAF92_004905"
     CDS             1828..3147
                     /locus_tag="LAF92_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBI9793792.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="LAF92_004906"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="LAF92_004906"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBI9793793.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPFIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..>5032
                     /locus_tag="LAF92_004907"
     CDS             4926..>5032
                     /locus_tag="LAF92_004907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI9793794.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVM"
BASE COUNT         1303 a         1177 c         1201 g         1351 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgcac ccgtttcggg acaatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcgctcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaacccac gtcaatgaaa
      841 tgagtaaccc agggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttatctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg tgtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc tctggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taagtcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact agttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcggctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcgtgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattaa aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc tttcattcgt gctaaagccg
     3601 cagaaattgc ccttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctacc atttctcgat taacagtatt gatatcgcta
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 actatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaagtcct gctggattct gatttaggca
     4141 gcagtttgcc tttaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagcgg cattgccgaa gtaggccata tctcccgtct gattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtttg cgggtgaagt
     4321 ttctattaca gataagaaat accgtatcgc aaattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactgac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cgcagtagtg
     4621 attatgtttt ccgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggataatg ccgccgatgg catttttttc cccgcccttg
     4981 agcaaaatat gatgggtgcg gtgttaatta acgaaaatga tgaagtgatg tt
//
LOCUS       DAEVZA010000230         5005 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEVZA010000230 DAEVZA010000000
VERSION     DAEVZA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.230"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..349)
                     /gene="kdsB"
                     /locus_tag="LAF92_004908"
     CDS             complement(<1..349)
                     /gene="kdsB"
                     /locus_tag="LAF92_004908"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBI9793795.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNL"
     gene            complement(346..528)
                     /gene="ycaR"
                     /locus_tag="LAF92_004909"
     CDS             complement(346..528)
                     /gene="ycaR"
                     /locus_tag="LAF92_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBI9793796.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(580..1812)
                     /locus_tag="LAF92_004910"
     CDS             complement(580..1812)
                     /locus_tag="LAF92_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBI9793797.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(1849..2835)
                     /gene="lpxK"
                     /locus_tag="LAF92_004911"
     CDS             complement(1849..2835)
                     /gene="lpxK"
                     /locus_tag="LAF92_004911"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBI9793798.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(2832..4580)
                     /gene="msbA"
                     /locus_tag="LAF92_004912"
     CDS             complement(2832..4580)
                     /gene="msbA"
                     /locus_tag="LAF92_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBI9793799.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(4617..>5005)
                     /locus_tag="LAF92_004913"
     CDS             complement(4617..>5005)
                     /locus_tag="LAF92_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBI9793800.1"
                     /translation="DGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSS
                     SLPLIQRVNGKVALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQG
                     WRISSLREQILPRWYHQWFGVPVDNG"
BASE COUNT         1264 a         1382 c         1199 g         1160 t
ORIGIN      
        1 cgaggttatc agcaacctga cgaatgattg tcgcagggat catcggttca tcaccctgca
       61 cattaacgat caccgtgtcg tcgctgaatg cgcatttttc gacaacttcc gccaggcgtt
      121 ctgtccctga ctggtgatcg gcgcgcgtca tacatacttc accgccagcg gcttcaacgg
      181 cgcgggcgac atcctcatga tcggttgcca cgatgatgcg atcggcacct gattcgcgag
      241 cacgttcaag aacatgaaca atcatgggtt tgccattaat atcaaccaat ggtttaccgg
      301 gcagacgcgt tgacgcgtag cgcgcgggaa taatgaccac aaaactcatg atttactctc
      361 atcagcagtc agcacgcggg cttccgtttc cagcaacact ggaatgccat cacgcagcgg
      421 gaaagccagg ttgtccagtt tgcaaataag ttcttgtttt tcttggttat accaaagttt
      481 tccgttgcaa accgggcagg caatgatttc aagcagacga tgatccatag ttcctccaga
      541 tggatcgggt tatgaatgca taaatcttat cataatcatt tatgcgacgg ggtctatttc
      601 ccagccagtg cggcaatcag taaagagacc ttgcgggcag cgtcctaatg tcacccgcgt
      661 tgcctgctgc cagctcgcga aatcagtaat cgcctgacgt aacccttttt gcagcattgt
      721 cgttggttta atgccttcct gtaaccacag agagataact tcaaggatgc ctgtctggcg
      781 atgcattttg gcatccattc gcccaactaa ttgcccacga tgtaataatg gcagaacaaa
      841 atagccatac tggcgtttcg gcgctggggt atagcactcc agccggtagc taaaatcaaa
      901 aagctgctct gcgcgtttgc gatcccagac aacaggatca aaaggcgaaa gtaccgcgct
      961 gtgcgttgca gtgagctttc cggccagcgc acgctcgagt agcggcagca aatcagcatg
     1021 cagccaaaga ttgcccaatt tttcaacatg cacagcaatg atttgctgct gttcagccct
     1081 cgcttcgcgc cacgccgcca gtgccggacg tttcagccga tagtaatctg ccagccactg
     1141 ttcgcggaat attcccaggc tacgcgcact gttatccagc atgatgattt ctgcttctgt
     1201 ttgcgaaacg agatcgcgct catcatccca gtcaggcatg acacggtggg ttaaatcata
     1261 aacgcgctgg aagttgcgcc gttcaatcac catcaccttt ccggcagtaa ataaaccttc
     1321 cagatgccgt ttatgcggct tccattccca ccagccgctt gcacctttac gaggatgctc
     1381 aaaatcggct gaacgtaccg gccccttatc atgaatatgc tgaattaact gtgcaatttc
     1441 cgcctcatgt tcctgcatcc aggcgtcttt gtatttccag cccatttttt caggtgccag
     1501 catgcggtgg cgaataagac gaaagtcgct acgcggcata aagcaggctt catgcgccca
     1561 gtattccatt aattcgccac gcgccagaga ctcatccagc cactgggcag gataatttcc
     1621 cagacgactg aaaagcacca gatatggact acgggcaaca atattgatgg tatcgatttg
     1681 cagcaaggac atgcgggaga tcgttgccgg aatatcctcc aacgacgctt gacggcgggg
     1741 tttgtttaac aggccttgtg cggcaaggtg aagattacgc gcatcagcaa gggagaggtg
     1801 cggcagcgac attcatgact ccatcaatcg aacgctgccg cggcgtaact agttgccaga
     1861 agccagcgag gttagttgcg taagcagttt cgctggttca tcacctgaaa gctgtgcgtc
     1921 tacaggcaaa taccaccaat tttcttctgc aaaggcccgg catttcaccg catctttttc
     1981 agtcattacc agcgtttgcc cggcgcttac caacgcactg acatccgcat ggttcaaaga
     2041 ctgatgatcg gccagcggta cacatttttc cggttgtacg ccacacatct tcagcgtggc
     2101 aaaaaagcgc ggcggatgcc caatccccgc catcgccact acatgttcaa gctgagcaac
     2161 gtcacaacgc gtaccggtac gtaaattcac cgcctgaccc ggcagcagat gcatggggat
     2221 ttcaccgctg cgagggacac caccgttgac gattaccgca tcaaccgact ttaagcgccc
     2281 cgctcgctca cgcattggcc ccgccggcaa ccaccagcca ttgccaaagc gacgcacacc
     2341 atcaataacg acaatttcca catcacgcgc cagacggtaa tgctgtaaac cgtcgtcggt
     2401 tacgatgatc tgcacatcag ggtgttgcgc cagaatggct tttaccgcat cagaacgaac
     2461 gggagaaacc gcaacaggcg catcagtgcg ttgataaatc aacacaggtt catcacccgc
     2521 ctgtgctgtt gtggtatctg ccgacaataa cagcggataa gattcagcct taccaccata
     2581 tccccgcgat acgaccccca ctcgaattcc gcgctgttgc aactgttcca ccagccagac
     2641 aacgaccggg gtttttccgt tgccgcctgc ggtgagatta ccaaccacga caaccggtac
     2701 gggggcacgc caggcgcgct tcagttttag tttatagcaa agacggatcg cgccactcac
     2761 caggccatac aaccaggaga gtggcagcaa tagccgccac aaaggggatt caccagacca
     2821 gattttttcg atcattggcc aaactgcatt ttgtgaagtt gcgcgtaaac gccgcggtgc
     2881 tcaagcaaat cgttatgcgt accgcgttcc acaatgacac catcctcgac gaccacgatt
     2941 tcgtctgcct tttcaatggt agacaagcgg tgggcaatca ccagagaggt acggtttttc
     3001 tgcaactcat ccagtgccgc ctgaatcgca cgttcggatt cggtatccag agccgaggta
     3061 gcttcatcca gaatcagaat cgggctatca cgcagcaagg ctcgagcgat agcaatacgc
     3121 tgacgctgac cgccagagag cagcacgccg ttttcaccaa tcactgtatc gagaccgtta
     3181 tccatcttat tgatgaagtc catggcgtag gccatacgcg ccgcttcttc aatttgctca
     3241 cggctgtact gttcagtccg tgcgtaagca atgttgttag caaccgtatc gttaaacaga
     3301 tggacattct gcgacaccag agcaacctgg ttacgtaacg acgccagggt atactcgcgc
     3361 agatcgtgac catccatcag gatttcgcct tcatcaatat cgtaaaaacg agtgatcagg
     3421 ctggcgatgg ttgatttacc tgaaccagag cgtccaacca gagcaaccgt cttccctgcc
     3481 ggaattttca ggttgatgtt acgcaatgca ggtacgtcac gtcccggata agtaaaggtg
     3541 acattgcgga attccacgtc gccagtcgca cgctcgatca cgcgcttacc ttcgtctttc
     3601 tcctgctcac tgtccagaat ggtaaacagc gtctgacaag ccgccatacc gcgctggaac
     3661 tgggcgttaa cgttggtcag tgatttcagc ggacgcatca gtgcaatcat tgaagagaaa
     3721 acaacggtaa tcgtaccggc agtcaggcta tccatgacac ttgggaagct cgccgcatac
     3781 agaacaaacg ccagcgccaa agaggcgatc agctgaatga tcggatcaga gatggaagag
     3841 gctgaaacca ttttcatccc ctgaagacgc attcggttgc tgactttatc aaagcgtttc
     3901 gtttccactt cctgaccacc gaaaatcaat acttctttgt ggcccttcag catttgttcg
     3961 gcgctggtgg tcacctgccc catggtgttc tgcatgtttt tactgatgtt gcgaaaacgc
     4021 ttcgatacta cgcgaatcgc aatcgaaaca atcggtgcca gcacaatcaa aatgatcgac
     4081 agttgccaac tgtaatagaa catcatgatg aacaggccga tgattgacgc accttcacgc
     4141 acaaccgtta tcagtgcgcc ggaagaagaa gaagcaacct gttcggaatc gtaggtgata
     4201 cgtgacaaca gcgtccccgt tgactgtttg tcaaaaaatg aaaccggcat tcccatcatg
     4261 tgaccaaaca ggcggcgacg catggtcatt accacctttc ctgataccca ggagatacag
     4321 tagctggaga catagctggt gataccacgt aaaatcatca gcccgatcac caccagcggc
     4381 atccacacca gcacggagcg atctgtttta ccaaagccat catcaagaag tggcttaagg
     4441 agcgataaca tgaaggtatc gctggctgcg ttgaggatta acgctacgcc cgccacgatc
     4501 agacccgctt taaaaggcgc aatggttggc cacagtcggc ggaacgtctg ccacgtagag
     4561 agatctttgt cgttatgcat tcaaaaaacc agcatttgtt gaaatagccg catattctac
     4621 ccgttatcca ctggcacgcc aaaccactga tgataccaac gaggtaaaat ttgctctctg
     4681 aggctgctaa tccgccagcc ttgcgctgaa aaattgaccg tcacttgacc ctgatgtgga
     4741 gtatcaagcc attgatatcc ttgctgttga tagcgatgct taactttatt agagggcaat
     4801 cgccatgcgt tatagcgcga tgccgatgcg agtgccactt ttccattcac tcgctgaatt
     4861 aatggcaacg atgatgaggt attactgcca tggtgaggta cctgaagcaa tgttgtctgc
     4921 acttgctgcc agtaacggct tagcatcttt tgttcagcgg ggacttcaat atcaccggtt
     4981 agaagaatgc tattcgtccc gtcat
//
LOCUS       DAEVZA010000231         4966 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEVZA010000231 DAEVZA010000000
VERSION     DAEVZA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.231"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..705
                     /locus_tag="LAF92_004914"
     CDS             <1..705
                     /locus_tag="LAF92_004914"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="catalyzes the synthesis of 2-methylcitrate from
                     propionyl-CoA and oxaloacetate; also catalyzes the
                     condensation of oxaloacetate with acetyl-CoA but with a
                     lower specificity; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HBI9793801.1"
                     /translation="NGERIQPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAE
                     HEFNASTFTSRVIAGTGSDMYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEA
                     DIRKRVESKEVVIGFGHPVYTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMW
                     ESKKMFPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSAN
                     YVGPEDRQFVALDKRQ"
     gene            739..2190
                     /gene="prpD"
                     /locus_tag="LAF92_004915"
     CDS             739..2190
                     /gene="prpD"
                     /locus_tag="LAF92_004915"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HBI9793802.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            2230..4116
                     /gene="prpE"
                     /locus_tag="LAF92_004916"
     CDS             2230..4116
                     /gene="prpE"
                     /locus_tag="LAF92_004916"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HBI9793803.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            4454..>4966
                     /locus_tag="LAF92_004917"
     CDS             4454..>4966
                     /locus_tag="LAF92_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBI9793804.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWL"
BASE COUNT         1064 a         1349 c         1437 g         1116 t
ORIGIN      
        1 aacggcgaac gcatccagcc ggaaaccgat gacgactcca tcggtggtca cttcctgcat
       61 ctgctgcacg gcgaaaagcc gtcgcaaagc tgggaaaagg cgatgcatat ttcgctggtg
      121 ctgtacgccg aacacgagtt taacgcctcc acctttacca gccgggtgat tgcgggcact
      181 ggctctgata tgtattccgc gattattggc gcgattggcg cactgcgcgg gccgaagcac
      241 ggcggggcga atgaagtgtc gctggagatc cagcaacgct acgaaacgcc ggacgaagcc
      301 gaagccgata tccgcaagcg ggtggaaagc aaagaagtgg tcattggttt tggtcatccg
      361 gtttatacca tcgccgaccc gcgccaccag gtgattaaac gtgtggcgaa gcagctctcg
      421 caggaaggcg gctcgctgaa gatgtacaac atcgccgatc gcctggaaac ggtgatgtgg
      481 gagagcaaaa agatgttccc caatctcgac tggttctccg ctgtttccta caacatgatg
      541 ggcgttccca ccgagatgtt cacaccactg tttgttatcg cccgcgtcac cggctgggcg
      601 gcgcacatta tcgaacaacg tcaggacaac aaaattatcc gtccttccgc caattatgtt
      661 ggaccggaag accgccagtt tgtcgcgctg gataagcgcc agtaaacctc tacgaataac
      721 aataaggaaa cgtacccaat gtcagctcaa atcaacaaca tccgcccgga atttgatcgt
      781 gaaatcgttg atatcgtcga ttacgtcatg aactacgaaa tcagctccaa agtagcctac
      841 gacaccgcac attactgcct gctcgacacg ctcggctgcg gtcttgaagc tctcgaatac
      901 ccagcctgta aaaaactgct ggggccaatt gtccccggca ccgtcgtacc taacggcgtg
      961 cgcgtccccg gaactcagtt ccagctcgac cccgtccagg cggcatttaa catcggcgcg
     1021 atgatccgtt ggctcgattt taacgatacc tggctggcgg cggagtgggg gcatccttcc
     1081 gacaacctcg gcggcattct ggcaacggcg gactggcttt cgcgcaacgc ggtcgccagc
     1141 ggcaaagcgc cgttgaccat gaaacaggtg ctgaccggaa tgatcaaagc ccatgaaatt
     1201 cagggctgca tcgcgctgga aaactccttt aaccgcgttg gcctcgacca cgttctgtta
     1261 gtgaaagtgg cttccaccgc cgtggtcgcc gaaatgcttg gcctgacccg cgaggaaatt
     1321 ctcaacgccg tttcgctggc gtgggtggac ggtcagtcgc tgcgcaccta tcgccatgcg
     1381 ccgaacaccg gcacgcgtaa atcctgggcg gcgggcgatg ccacttcccg cgcggtacgt
     1441 ctggcactga tggcgaaaac gggcgaaatg ggttacccgt cagccctgac tgcgccggtg
     1501 tggggattct acgacgtctc ctttaaaggt gaatcgttcc gcttccagcg cccgtacggt
     1561 tcttacgtga tggaaaatgt gctgttcaaa atctccttcc cggcggagtt ccactcccag
     1621 acggcagttg aagcagcgat gacgctctat gaacagatgc aggcagcagg caaaacggcg
     1681 gcggatatcg aaaaagtgac cattcgcacc cacgaagcct gtattcgcat catcgacaaa
     1741 aaagggccgc tcaataaccc ggcagaccgc gatcactgca ttcagtacat ggtggcgatc
     1801 ccgctgctat tcgggcgctt aacggcggca gattacgagg acaacgttgc gcaagataaa
     1861 cgcattgacg ccctgcgcga gaagatcaat tgctttgaag atccggcatt taccgctgac
     1921 taccacgacc cggaaaaacg cgccatcgcc aatgccatta cccttgagtt caccgacggc
     1981 acacgatttg aagaagtggt ggtggagtac cccattggtc atgctcgccg ccgtcaggat
     2041 ggtattccga aactggtcga taaattcaaa atcaatctcg cgcgccagtt cccgactcgc
     2101 caacagcagc gcattctgga ggtttctctc gacagaactc gcctggaaca gatgccggtc
     2161 aatgagtatc tcgacctgta cgtcatttaa gtaaacggcg gtaaggcgta agttcaacag
     2221 gagagcatta tgtcttttag cgaattttat cagcgttcga ttaacgaacc ggagcagttc
     2281 tgggccgagc aggcccggcg tattgactgg cagacgccct ttacgcaaac gctcgaccac
     2341 agcaatccgc cgtttgcccg ttggttttgt gaaggccgaa ccaatttgtg ccacaacgct
     2401 atcgaccgct ggctggagaa acagccagag gcactggcat tgattgccgt ctcttcggaa
     2461 acagaagaag agcgcacctt tacctttcgt cagcttcatg acgaagtaaa cgcggtggcg
     2521 tcaatgctgc gctcgctggg cgtgcagcgt ggcgatcggg tgctggtgta tatgccgatg
     2581 attgccgaag cgcatattac tctgctggcc tgcgcgcgca ttggcgctat tcattcggtg
     2641 gtgtttgggg gatttgcctc gcacagcgtg gcggcgcgaa ttgatgatgc taaaccggtg
     2701 ctgattgtct cggctgatgc cggagcgcgc ggtggcaaaa tcattcccta taaaaaattg
     2761 ctcgacgatg cgataagtca ggcgcagcac cagccacgcc atgttttgct ggtggatcgc
     2821 gggctggcga aaatggcacg cgtcagcggg cgggatgtcg atttcgcgtc gttgcgccat
     2881 caacacatcg gcgcgcgggt accggtggcg tggctggaat ccaacgaaac ctcctgcatt
     2941 ctctacacct ccggcacgac cggcaaacct aaaggcgtgc agcgtgacgt cggcggatat
     3001 gcggtggcgc tggcgacctc gatggacacc atttttggcg gcaaagcggg cggtgtgttc
     3061 ttttgtgctt cggatatcgg ctgggtggtg gggcattcgt atatcgtcta cgcgccgctg
     3121 ctggcgggga tggcgactat cgtttacgaa ggattaccga cctggccgga ctgcggcgtg
     3181 tggtggaaaa tcgtcgagaa atatcaggtg agccggatgt tctcagcgcc gaccgccatt
     3241 cgcgtgctga aaaaattccc taccgctgaa attcgcaaac acgatctctc gtcgctggaa
     3301 gtgctctatc tggctggaga accgctggac gagccgaccg ccagttgggt gagcaatacg
     3361 ctggatgtgc cggtcatcga caactactgg cagaccgaat ccggctggcc gattatggcg
     3421 attgctcgcg gtctggacga caggccgacg cgtctgggaa gccccggcgt gccgatgtat
     3481 ggctataacg tgcagttgct taatgaagtc accggcgaac cgtgtggcgt caacgagaaa
     3541 gggatgctgg tggtggaagg gccgctgccg ccggggtgta ttcagaccat ctggggcgat
     3601 gacgaccgct ttgtgaagac gtactggtcg ctgttttccc gtccggtgta cgccaccttt
     3661 gactggggca tccgcgatgc tgacggttat cactttattc tcgggcgcac tgacgatgtg
     3721 attaacgttg ccgggcatcg gctgggcacg cgcgagattg aagagagtat ttccagtcat
     3781 ccgggcgttg ccgaagtggc ggtggttggg gtgaaagatg cgctgaaagg gcaggtggcg
     3841 gtagcgtttg tcattccgaa agagagcgac agtctggaag atcgtgatgt ggcgcattcg
     3901 caagagaagg cgattatggc gctggtggac agccagattg gcaactttgg ccgcccggcg
     3961 cacgtctggt ttgtctcgca attgccaaaa acgcgatccg gaaaaatgct gcgccgcacg
     4021 atccaggcga tttgcgaagg acgcgatcct ggagatctga cgaccattga tgatccagcg
     4081 tcgttggatc agatccgcca ggcgatggaa gagtaaggta ttgtcggatg cgtcgcgtgg
     4141 cgcatccgac accatgcgcc gatgcctgat gcgacgctta ccgcgtctta ttatgcctac
     4201 aggcctgaac cgtaggtcgg ataaggcgct cgcgtcgcat ccgacacccc gcgccgatgc
     4261 ctgatgcgac gctgacgcgt cttatcaggc ctacccacta cccactgttt ttacaccgat
     4321 aatttttccc ccaccttttt gcactcattc atataaaaaa tatatttccc cacgaaaacg
     4381 attgcttttt atcttcagat gaatagaatg cggcggattt tttgggtttc aaacagcaaa
     4441 aagggggaat ttcgtgtcgc aagataacaa ctttagccag gggccagtcc cgcagtcggc
     4501 gcggaaaggg gtattggcat tgacgttcgt catgctggga ttaaccttct tttccgccag
     4561 tatgtggacc ggcggcactc tcggaaccgg tcttagctat catgatttct tcctcgcagt
     4621 tctcatcggt aatcttctcc tcggtattta cacttcattt ctcggttaca ttggcgcaaa
     4681 aaccggcctg accactcatc ttcttgctcg cttctcgttt ggtgttaaag gctcatggct
     4741 gccttcactg ctactgggcg gaactcaggt tggctggttt ggcgtcggtg tggcgatgtt
     4801 tgccattccg gtgggtaagg caaccgggct ggatattaat ttgctgattg ccgtttccgg
     4861 tttactgatg accgtcaccg tcttttttgg catttcggcg ctgacggttc tttcggtgat
     4921 tgcggttccg gctatcgcct gcctgggcgg ttattccgtg tggctg
//
LOCUS       DAEVZA010000232         4878 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEVZA010000232 DAEVZA010000000
VERSION     DAEVZA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.232"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            17..544
                     /locus_tag="LAF92_004918"
     CDS             17..544
                     /locus_tag="LAF92_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI9793805.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            602..835
                     /locus_tag="LAF92_004919"
     CDS             602..835
                     /locus_tag="LAF92_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBI9793806.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            896..2919
                     /locus_tag="LAF92_004920"
                     /pseudo
     CDS             896..2919
                     /locus_tag="LAF92_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            2988..3422
                     /gene="psiB"
                     /locus_tag="LAF92_004921"
     CDS             2988..3422
                     /gene="psiB"
                     /locus_tag="LAF92_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBI9793807.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3419..4138
                     /locus_tag="LAF92_004922"
     CDS             3419..4138
                     /locus_tag="LAF92_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBI9793808.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(4159..4338)
                     /locus_tag="LAF92_004923"
     CDS             complement(4159..4338)
                     /locus_tag="LAF92_004923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HBI9793809.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQAVYG"
     gene            4418..4576
                     /locus_tag="LAF92_004924"
     CDS             4418..4576
                     /locus_tag="LAF92_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI9793810.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
BASE COUNT         1097 a         1287 c         1518 g          976 t
ORIGIN      
        1 cagaaggaga cacatcatgg cagttcgtgg cattaacaag gtcattcttg tcggtcgtct
       61 gggaaaagat ccggaagtcc gttacatccc caacgggggc gcagtggcaa acctgcaggt
      121 ggccacgtca gaaacctggc gcgacaaaca gacgggggaa atgaaggagc agacggaatg
      181 gcaccgcgtg gtgctgttcg gcaagctcgc ggaagtggca ggtgaatatc tgcgtaaggg
      241 ggcgcaggtc tacattgaag gccagcttcg cacccgtagc tgggaagata acggtattac
      301 ccgttacgtc accgaaatcc ttgttaaaac cacgggcacc atgcagatgc tggggagtgc
      361 accacagcag aacgctcagg tgcaaccgca gcctcagcag aatggtcagt cacagagtgc
      421 tgacgcgacg aaaaaaggga gcgcgaaaac gaaaggccgt ggacgtaagg ctgcgcagcc
      481 ggagcctcag ccgcaaccac cggaggggga ggattacggg ttttcagacg atatcccgtt
      541 ctgactggcc tgactgtgat aaccgccccg tcctgcgcgg ggcatcaccg gagatatgag
      601 gatgagcgaa tatttcagaa tacttcaggg actgcctgac ggtcctttta cccgcaaaca
      661 ggccgaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgacc acggcgaaca
      721 gtttcgcctg gttgtccgta ataacggaac aatggtctgg cgtacctgga attttgagga
      781 cggtgccggg tactggatga accatgtcat ccgtgatttc gggatcctta agtaagagag
      841 gtgccggacg gggatcacgt ccggcagaca ttaactacac aataagggga tagtcatgtc
      901 agtaacaaag tctgaaccaa aatctacccg taaagcaagc cgtaaacctg caaaaacgca
      961 ggaaactgtc ctgtctgccc tgctggcgca gacggaggaa gtgagcgtgc cgctggcctc
     1021 gctgattaag tcaccactga atgtgcgcac ggtgccgtat tctgcggagt ccgtcagcga
     1081 actggctgat tccattaagg gggtcggcct gctgcagaat ctggttgttc atgccctgcc
     1141 aggtgaccgc cacggtgtcg ccgcaggtgg tcgccgactg gcagcactca acatgctggc
     1201 agagcgtggc atcattccgg ctgactggcc tgtccgcgtg aaaattattt cgcaggaact
     1261 ggcgacggcc gcatcgatga ccgagaacgg tcatcgtcgg gatatgcacc cagccgaaca
     1321 gattgccgga ttctgtgcaa tggcgcagga aggcaaaaca cctgcacaaa tcggtgattt
     1381 gctgggctat tcaccccgcc acgttcagcg aatgctgaaa ctggctgacc ttgcgcctgt
     1441 catcctcgat gcgctggcag aagacctcat caccaccgag cactgtcagg cgctggcgct
     1501 ggagaacgac accgcgcgtc aggtgcaggt gtttgaagcc gcctgtcagt cgggatgggg
     1561 cggtaaaccg gaagtacaga ccattcgtcg tctggtgacc gaaagtgaag tggcggtggc
     1621 agggaacagt aaattccgct tcgtgggggc tgatgccttc tcgccagatg aactgcgcac
     1681 tgacctgttc agcgacgacg agggtggcta tgtggactgc gtggcgctcg atgccgccct
     1741 gctggaaaaa ctccaggctg ttgctgaaca ccttcgggaa gccgaaggct ggggatggtg
     1801 cgccggacgc atggagcctg tcggtgagtg ccgtgaggat gccggaacat accgctgtct
     1861 gccggagccg gaagcggtgc tgacggaggc ggaagaagaa cgtctgaacg aactgatgac
     1921 gcgttacgac gcgctggaaa accagtgtga ggaatctgac ctgctggaag cagaaatgaa
     1981 gctgatgcgc tgcatggcga aggtcagggc gtggacgccg gagatgcgtg ccggaagcgg
     2041 tgtggtggtg tcctggcgtt atggcaacgt gtgtgtccag cgtggtgtgc agttgcgcag
     2101 cgaggatgat gcggctgacg acgctgaccg cacggaacag gtgcaggaga aagcgcccgt
     2161 ggaggaaatc agtctgccac tgctgacgaa aatgtcttcc gaacgcacgc tggcagtcca
     2221 ggcagcactg atgcagcaac cggacaaatc tctggcactg ctggcatgga cgctctgccc
     2281 tgatgaatcc cctaatgatt tttaccaaaa tcattagggg attcatcagc gctctgcctg
     2341 aatgtgtttg gcagcggagc gtacagtaaa ccagcacaaa tcagcctgga atgtaaacat
     2401 tattcgctga ccagcgatgc gccatcgggg aaggacggtg ccgcattcct ggcgctgatg
     2461 gcagaaaaag cccgtcttgc agccctgtta ccggagggat ggtcacggga tatgacgaca
     2521 ttcctgtcac tcagccagga ggtactgtta tccctgctca gtttctgcac cgcgtgcagc
     2581 atccacggcg ttcagacccg cgagcatggt cacacgtcac gcagtccgct ggactcgctg
     2641 gagagcgcta tcggattcca catgcgcgac tggtggcagc cgacgaaagc aaacttcttc
     2701 ggacacctgc aaaagccgca gattatcgat gccctgaatg atgccggact gtccggtgct
     2761 gcgcgggacg cggagaaaat gaagaaaggc gatgccgctg aacatgcaga gttccacatg
     2821 aaagacaacc gctgggtgcc tggctggatg tgtacaccac gtccacaggc agagacggaa
     2881 accacggagt accgtgacga ccaggcagaa gccgcctgac cgccagtcat aaaccattta
     2941 tcacgccgcc ctgccggaga cggggcggca gcaagggaga taccgtgatg aaaactgaac
     3001 tgaccctgaa tgtattacag accatgagcg cacaggaata tgaagatatc cgggctgccg
     3061 gaagtgatga acgtcgtgaa ctcacacatg ccgtgatgcg ggagctggat gcgccggata
     3121 actggacgat gaacggcgag tatggcagcg agttcggcgg ctttttcccc gtccaggtcc
     3181 gtttcacgcc tgcccacgaa cgttttcacc tggcgttatg ttcaccgggc gatgtctctc
     3241 aggtctgggt gctggttctg gtgaatgccg gtggtgagcc gtttgcggtg gttcaggtac
     3301 agcggcggtt tgcaccggaa gccgtcagcc attcactggc actggcggcg tcactggacg
     3361 cgcaggggta cagcgtcagt gacatcatcc atatcctgat ggcggaagga ggtcaggcat
     3421 gagcgcacgt tcacaggcac tggttcccct cagcgcagag caacaggccg catggcgggc
     3481 ggtggcggag acagaaaaac gccgccacca gggaaacacg ctggcggaat atccgtatgc
     3541 gggggcgttt ttccgctgtc tgaacggaag ccgcaggata tcgctgtctg acctgcgatt
     3601 tttcatgccg tccctgaccg cagaagaact gcgcggcaac cgttctcagt ggctgtatgc
     3661 cgtcgatgtg ctgattgaaa ctcagggcga ggtctgcctt cttccccttc ccggcgatgc
     3721 agcagagcgg ctgttcccgt cggtgcggtt ccgtgtcagg gagcgcagtc gccacaaatc
     3781 cgcactggtc atgcaaaagt acagccgcca gcaggcgagg gaggcagagc agaaagcccg
     3841 tgcgtaccag gcgctggtcg ctcaggctga aatcgaactg gcattccatt cgccggaaac
     3901 agtcggaagc tggcatgcac gctggagtga ccgggtggca gagcatgacc tggaaaccct
     3961 gttctggcag tggggtgagc gttttccgtc actggccgga atggaacgct ggcagtggca
     4021 ggatatgccg ttctggcagg ttattgcgga agccagtctt gcagcaagag aggccggtca
     4081 cgctgtccgt gagatggagc ggtggatggt gccgaacaaa ctccgggagg cggcgtgatg
     4141 cggcaacaat cacacagatt acccgtaaac agcctgaatg agcgggttat tttcaggaaa
     4201 agtgagtgtg gtcagcgtgc aggtatatgg gctatgatgt gcccggcgct tgaggctttc
     4261 tgcctcatga cgtgaaggtg gtttgttacc gtgttgtgtg gcagaaagaa gatagccccg
     4321 tagtaagtta attttcatta accaccacga ggcatcccta tgtctagtcc acatcaggat
     4381 agcctcttac cgcgctttgc gcaaggagaa gaaggccatg aaactaccac gaagttccct
     4441 tgtctggtgt gtgttgatcg tgtgtctcac actgttgata ttcacttatc tgacacgaaa
     4501 atcgctgtgc gagattcgtt acagagacgg acacagggag gtggcggctt tcatggctta
     4561 cgaatccggt aagtagcaac ctggaggcgg gcgcaggccc gccttttcag ggctgatgct
     4621 ggtctgactg cactgaagcg cctttataaa ggggctgctg gttcgccggt ggcccctttc
     4681 tccttgctgg cattgtacgg gcatgaacaa ccagactgcc aggaaggccg tttccctccg
     4741 ggaacgggga tgaacgtgcg cctgcggcgc ccgttctttt cccccgcctt ctctgtttat
     4801 gacttaccag aaatcacatc accttccctt tgattattct ctttttcctc ctgctctgac
     4861 tctgattact gctctgac
//
LOCUS       DAEVZA010000233         4798 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEVZA010000233 DAEVZA010000000
VERSION     DAEVZA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.233"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..124
                     /locus_tag="LAF92_004925"
     CDS             <1..124
                     /locus_tag="LAF92_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HBI9793811.1"
                     /translation="GKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            252..833
                     /gene="sodB"
                     /locus_tag="LAF92_004926"
     CDS             252..833
                     /gene="sodB"
                     /locus_tag="LAF92_004926"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBI9793812.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(979..2148)
                     /locus_tag="LAF92_004927"
     CDS             complement(979..2148)
                     /locus_tag="LAF92_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9793813.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTEMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(2314..2403)
                     /gene="ynhF"
                     /locus_tag="LAF92_004928"
     CDS             complement(2314..2403)
                     /gene="ynhF"
                     /locus_tag="LAF92_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBI9793814.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            2702..3727
                     /gene="purR"
                     /locus_tag="LAF92_004929"
     CDS             2702..3727
                     /gene="purR"
                     /locus_tag="LAF92_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBI9793815.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(3724..4656)
                     /locus_tag="LAF92_004930"
     CDS             complement(3724..4656)
                     /locus_tag="LAF92_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI9793816.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
BASE COUNT         1261 a         1298 c         1140 g         1099 t
ORIGIN      
        1 cggcaaattt attcagtcac cgcgcacagg tcaggaaatt caaatcactt ctctcagtga
       61 agactactgg cagcgccact atgttggcgc tcgtcgggta atgaccccaa aaacacttcg
      121 ctaaaacttt accctgttgt tacggcaaca gggtaagttc atcttttgtc tcacctttta
      181 atttgctacc ctatccatac gcacaataag gctattgtac gtatgcaaat taataataaa
      241 ggagagtagc aatgtcattc gaattacctg cactaccata tgctaaagat gctctggcac
      301 cgcacatttc tgcggaaact atcgagtatc actacggcaa gcatcatcag acttatgtca
      361 ctaacctgaa caacctgatt aaaggtaccg cgtttgaagg taaatcactg gaagagatta
      421 ttcgcagctc tgaaggtggc gtattcaaca acgcagctca ggtctggaac catactttct
      481 actggaactg cctggcaccg aacgccggtg gcgaaccgac tggaaaagtc gctgaagcta
      541 tcgccgcatc ttttggcagc tttgccgatt tcaaagcgca gtttactgat gcagcgatca
      601 aaaactttgg ttctggctgg acctggctgg tgaaaaacag cgatggcaaa ctggctatcg
      661 tttcaacctc taacgcgggt actccgctga ccaccgatgc gactccgctg ctgaccgttg
      721 atgtctggga acacgcttat tacatcgact atcgcaatgc acgtcctggc tatctggagc
      781 acttctgggc gctggtgaac tgggaattcg tagcgaaaaa tctcgctgca taataactga
      841 tggcaaatgc agcattgcct gatgcgcttc gcttaccatg ccaacgcgga tgaggcgtta
      901 acagcgcatt cggcaagata aatcgcacgt tgtcagcaac tgtaacgcag aaggttatcc
      961 ttctgcgttt ttgtttaatt agctgttagc aacgcaaact gtttcaggtt gttttctggc
     1021 tgacataaac accagcaata atgccagtcc cgcgacaatc gctcccatca ccggcacaaa
     1081 gctgtatccc agcccagcgg aaattaccgc accaccagca gctgctccca gcgcatttcc
     1141 aagattaaag gcaccaatat tgactgatga agacagacct ggcgcttcac tggcgacacg
     1201 catcacgcgc atctgtaacg gcggtacgac cgcaaaggtt gctgcgcccc acaccaccat
     1261 gctaatagct gcgccgaact cattgcgggc caggaacggg attgccagca taatcaccat
     1321 caacaacaac aaaaagcctt tcaacgtgcc gttaactgaa cgatctgcca gtttgccgcc
     1381 gagatagtta ccgatagaga atccgacacc aatcagcacc agcatttccg tgacgaacac
     1441 cggtgttgcg tgggtaatac tttgcagtac cggagagata taggtgtaga gagtaaacat
     1501 tgcaccagct cccagtaccg tcgtcagcaa tgcagacagc acctgcggac gcattaatac
     1561 cgccagctct tttttcactt caggtcgtgc ccctgcacca cctttaggta atgagaagaa
     1621 cagacttacc attgaaatca ctcccagccc cgccgttgcc agaaatgaca tccgccagcc
     1681 gatggtttca cccaaccagg tcgccgccgg cacgccaccg atatttgcca gggttaaccc
     1741 cataaacata gtggcaactg cgctggcctg tttatgtttt ggcaccacgc ttgcggccac
     1801 gactgaaccc aaaccaaaaa atgctccgtg attcaggctg gtcaaaatgc gtgaaagcat
     1861 cagggtcata taatccggcg cgatggcgga aagtacgttg ccgagcgtga aaattgccat
     1921 caggaaaatc aacgcactgc ggcgggcacg atgagaaagt agaagcgtca tcagcggcgc
     1981 gcccaccatt acgccaactg cataggcact gattaacatt ccggcagcgg gaatcgagac
     2041 atccacaccg cgcgcaatga cgggcaacaa gcccattggc gagaactccg ttgtcccgat
     2101 accaaacgcg ccaatcgcca gcgccagcaa cggatagtta attttcatgc cttatctcca
     2161 cctcttcgcg tcattacgcg atattcatta aagtggcgaa agcatgacag caatcacaaa
     2221 aaaatgaaaa taacaaaaag agaaaacact tttgccattt tgctaacaaa caggaaggag
     2281 atgcgaggga gaacgcgctc cctcgagagg aaatcagtgc agcgcggcag tcaaacccac
     2341 ggctacgatc aaaccgagga cgataatcgt tgttaccagt gaaaatttaa ggtcggtgct
     2401 catcaagttt tctccttttt tattaccaca caaaaagtga tattacgcat ttttacacac
     2461 tgtgatgaaa aaatctcccg tcatttataa tgataagtgt ttttaccact tccccttttc
     2521 gtcaagatcg gccaaaattc cacgcttaca ctatttgcgt actggccatt gaccccttcc
     2581 tgacgctccg tgtcgttttt ccggcgtacc gcaacacttt tgttgtgcgt aaggtgtgta
     2641 aaggcaaacg tttaccttgc gattttgcag gagctgaagt tagggtctgg agtgaaatgg
     2701 aatggcaaca ataaaagatg tagcgaaacg agcaaacgtt tccactacaa ctgtgtcaca
     2761 cgtaatcaac aaaacacgtt tcgtcgctga agaaacgcgc aacgccgtgt gggcagcgat
     2821 taaagaatta cactactccc ctagcgcggt ggcgcgtagc ctgaaggtta accacaccaa
     2881 gtctatcggt ttgctggcga ccagcagcga agcggcctat tttgccgaga tcattgaagc
     2941 ggttgaaaaa aattgcttcc agaaaggtta caccctgatt ctgggtaatg cgtggaacaa
     3001 tcttgagaaa cagcgggctt atctgtcgat gatggcgcaa aaacgcgtcg atggtctgct
     3061 ggtgatgtgt tctgagtacc cagagccgtt gctggcgatg ctggaagagt atcgccatat
     3121 cccaatggtg gtgatggact ggggtgaagc aaaagctgac ttcaccgatg cggtcattga
     3181 taacgcgttc gaaggcggct acatggccgg gcgttatctg attgaacgcg gtcaccgcga
     3241 aatcggcgtc atccccggcc cgctggaacg taacaccggc gcaggccgcc ttgccggttt
     3301 tatgaaggcg atggaagaag cgatgatcaa ggtgccggaa agctggattg ttcagggtga
     3361 ctttgaaccc gaatctggtt atcgcgccat gcagcaaata ctgtcgcagc cgcatcgccc
     3421 tactgccgtc ttctgtggtg gcgatatcat ggcaatgggc gcactttgtg ctgctgatga
     3481 aatgggtctg cgcgtcccgc aggatgtttc gctgatcggt tatgataacg tgcgcaacgc
     3541 ccgctatttt acgccggcgc tgaccacgat ccaccagcca aaagattcgc tgggtgaaac
     3601 agcgttcaac atgctgttgg atcgtatcgt caacaaacgt gaagaaccgc agtccattga
     3661 agtgcatccg cgcttgattg aacgccgctc cgtggctgac ggcccgttcc gcgactatcg
     3721 tcgttaatca cccgttgcgg gagtctcttc cggctcccgc agccactcct tattcagcgt
     3781 ctcactatcg ccgagatact caagcagcca ggttaacgca ggcgacatat cattttgctg
     3841 ccatgtcaga caacatgccg aatccgggaa ggggttttcc aattcaagtg ctacccactt
     3901 cccctcatta agccacggtt tggcgaaatg tgttggcacc atccctatgc ataatcctgc
     3961 cgagatacag gttgccgatg attcccagtc aggcacgaca actctttttt ggttatccag
     4021 caaccaggta atacgtttag gtagcgttcg cgaggtgtct tcgcgcacca acgacggcca
     4081 gttgcgcaac gtatcatcgc tgaatgggcc atccatcaac gccagcgggt ggtggctggc
     4141 aacaacgcaa ctccagctta gcatccccat atcccggaag gcataacgac cgcctaccgg
     4201 aatcgcgcgt gttgcgccaa tcgccagttc cacgcgcccg tcggaaagcg catcccagac
     4261 accgttgaac acttcctgaa agacaagaag ttcgacatca tcaaaatggc gataaaaatc
     4321 aacgatcatc tgccgtgtac gttctggcct gacaatatta tccactgcga tagctaactg
     4381 accgcgccag ccgttcgcta tctgctgaca ttgctggcgg gtgatctgca tttttttgac
     4441 aacagagcgc ccttctttga gaaaccacgc tccagcagcg gtcagctcca catcacggtg
     4501 ccgtcgttca aagagcggca ccgccagcca ctcttccagc tgacgcacgg tatagctgac
     4561 cgcagaagga acgcgatgca gctcctgtgc cgcagcgcta aaactaccat tacgcgctac
     4621 cgcatcaaca acttcgagtg aatattctga ccacatagtc tgcctgcaaa atttttgaaa
     4681 ccagtcatca aatattaccg tttcacaaca ctaatttcac tccctacact ttgcggcggt
     4741 gtttaattga gagatttaga gaatatacat gcaacctggg aaaagatttt tagtctgg
//
LOCUS       DAEVZA010000234         4775 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEVZA010000234 DAEVZA010000000
VERSION     DAEVZA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.234"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1230
                     /locus_tag="LAF92_004931"
     CDS             <1..1230
                     /locus_tag="LAF92_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI9793817.1"
                     /translation="LLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCA
                     VIANVSYLVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAA
                     SINLVATLAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVF
                     IGVEGAVVVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSM
                     AGLMVEMMGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQ
                     AAPSASLWLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPL
                     HKAVGVGACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVL
                     IGMLLIASVPATWMLVG"
     gene            1267..1989
                     /gene="folM"
                     /locus_tag="LAF92_004932"
     CDS             1267..1989
                     /gene="folM"
                     /locus_tag="LAF92_004932"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBI9793818.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(1986..2321)
                     /locus_tag="LAF92_004933"
     CDS             complement(1986..2321)
                     /locus_tag="LAF92_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBI9793819.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            2450..3169
                     /gene="rstA"
                     /locus_tag="LAF92_004934"
     CDS             2450..3169
                     /gene="rstA"
                     /locus_tag="LAF92_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBI9793820.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            3173..4474
                     /gene="rstB"
                     /locus_tag="LAF92_004935"
     CDS             3173..4474
                     /gene="rstB"
                     /locus_tag="LAF92_004935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBI9793821.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            4550..>4775
                     /locus_tag="LAF92_004936"
     CDS             4550..>4775
                     /locus_tag="LAF92_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="binds to DNA replication terminator sequences to
                     prevent passage of replication forks; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBI9793822.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFR"
BASE COUNT         1131 a         1194 c         1212 g         1238 t
ORIGIN      
        1 ttgctggcct ttgccatgct gatcctcacg cgcattcgtc ccgaacttga cggcggtatc
       61 tttacctatg cccgcgaagg gtttggcgag ctaatcggct tttgttccgc atggggatac
      121 tggctgtgcg cagtcatcgc caacgtctcc tatctggtga tcgttttttc cgcgttaagc
      181 ttttttacgg acacgccgga attgcgcctg ttcggcgatg gtaatacctg gcaatcgata
      241 gtcggtgcat cggctttatt gtggattgtt cactttttga tcctgcgcgg ggtgcaaacc
      301 gctgccagca ttaacctggt ggcgacactg gcaaaattgt tgccgctggg tctgtttgtt
      361 gtgctggcga tgatgatgtt caaactggat accttcaagc tcgacttcac cggacttgca
      421 cttggcgtac ccgtgtggga acaggtgaaa aacaccatgc tgatcaccct gtgggttttc
      481 attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc gtaataaacg tgatgttggc
      541 aaagcgacac tgctggcggt tctctccgct ctgggcgttt acttgctggt aacgctgctt
      601 tcgctgggcg tggtggcacg tcctgaactg gctgaaattc gtaacccgtc aatggccggt
      661 ctgatggtgg aaatgatggg accatggggc gaaatcatca tcgctgccgg tttgatcgtt
      721 tccgtttgcg gcgcgtactt aagctggacc atcatggcgg cagaagttcc gttcctggct
      781 gccactcata aagcattccc acgcattttt gcgcgccaga acgcacaagc tgcgccatcc
      841 gcctcgctgt ggttgaccaa catctgcgtg caaatttgtc tggtactcat ctggctgacc
      901 ggttccgatt acaacacgtt gctgaccatc gcctcagaaa tgattctggt gccctatttc
      961 ctggtcggcg cgttcttgct gaaaatcgca acccgtccac tgcataaagc ggtaggtgtc
     1021 ggtgcctgca tttatggctt atggttattg tatgcctctg gtccaatgca cttgctgtta
     1081 tccgttgttc tgtatgcgcc ggggctgctg gttttcctgt atgcgcgcaa aacgcataca
     1141 catgataatg tactgaaccg tcaggaaatg gtgctgattg gcatgctgtt aattgcctca
     1201 gttccggcga cctggatgct ggtgggataa cgttcatccc atcgttggat agcaaaggag
     1261 ataacgatgg gtaaagccca gcccttgcca atattaatta ctggcggagg tcgtcgcatc
     1321 ggcctcgccc tcgcctggca tttcattaat caaaagcaac cggtgattgt cagctatcgg
     1381 acgcactatc cagccattga tggactgatt aaggcaggtg cgcagtgtat tcaggctgat
     1441 ttttcgacca acgacggtgt gatggcgttt gccgatgaag tactaaaaag cacccatggt
     1501 ctgcgtgcta ttttgcataa cgccagtgcg tggatggcgg aaaaaccagg tacaccactg
     1561 gccgacgtac tggcttgcat gatgcagatc cacgttaata ccccatacct gctcaaccat
     1621 gcgctggaaa gattactgcg tgggcacgga cacgccgcca gcgatatcat tcactttacc
     1681 gattatgtgg tggagcgcgg tagcgacaaa catattgcgt atgctgcaag caaagcggca
     1741 ctggataata tgacccgctc gtttgcccgc aagctggcac cggaagtgaa agtgaattct
     1801 attgcgccat cgctgatcct gtttaatgaa catgatgatg ccgaatatcg acaacaggcg
     1861 ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag aagtgatcga cctggtcgat
     1921 tacttactta ccagctgctt tgtcaccgga cgcagtttcc cacttgatgg cggtcgtcat
     1981 ctgcgttaat gcagctttat ccagcatata atcaacaccc atgcgcttat cccccaacac
     2041 gcaacagaac caacaaaagc ggcgggcaag cgcatcattc cggtgaaata ccacagcgac
     2101 accaggtaaa caaaataggg aataatcgac cacatactaa aaatgatggt tgcgcgtaag
     2161 gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga taagcgcaaa ggtcgggaaa
     2221 agtggaatca gcccggcgat ataataattt ttcgtttttg ctaaaacacc aatcaacagc
     2281 actaccagcg caccgagcgc ggctttgatt accagcccca tctttttacc ttaacacttc
     2341 cataacaagt catcagtaga atacctgatg aaaacttgtt tagaaacgat tgatagtaag
     2401 taaaaacagc gcggtgtatt gtgacgtttt tatatctacc gtgaatgtta tgaacactat
     2461 cgtatttgtg gaagatgatg cggaagtcgg ttcactgatt gccgcgtacc tggcaaaaca
     2521 tgatatgcag gttaccgtag agccgcgcgg cgaccaggcc gaagaaacca ttttgcgaga
     2581 aaatccggat ttggtgttac tcgacatcat gctaccaggc aaggacggca tgaccatttg
     2641 tcgtgattta cgcgcaaagt ggtctggacc gattgttctt ctaacctctc tcgatagcga
     2701 tatgaaccac atcctggcac tggaaatggg tgcctgcgac tatattctca aaacgacgcc
     2761 ccctgctgtt ttgctagcgc gtttacgttt gcatttgcgt cagaatgagc aagccacact
     2821 gaccaaaggt cttcaggaaa cgtctctgac tccctacaaa gccctgcatt tcggcacgtt
     2881 gaccatcgat cccatcaacc gcgtagtcac cctggctaac actgaaatct cgctctcgac
     2941 agctgatttc gaattattgt gggaattagc tacccatgcc gggcaaatca tggaccgcga
     3001 tgcattgctg aaaaatttac gcggcgtcag ttatgacgga ctggatcgta gcgtggacgt
     3061 ggctatttcg cggttaagaa aaaaactgct cgataacgcc gcagaacctt atcgcattaa
     3121 aactgtgcgt aacaaaggct atctttttgc gcctcatgca tgggaataag cgatgaaaaa
     3181 actgtttatc cagttttacc tgttattgtt tgtctgcttc cttgtgatgt ctctgctggt
     3241 tgggttggtg tacaaattta ccgccgaacg cgcgggcaaa cagtcgctgg atgatttgat
     3301 gaacagttcg ctgtatctga tgcgcagcga attgcgtgag atccccccac acgactgggg
     3361 taagacgctg aaagagatgg atttaaatct ctctttcgat ctgcgtgtcg agccactgag
     3421 taaataccat cttgatgata tttccatgca ccgactgcgt ggcggcgaaa ttgtcgccct
     3481 ggacgatcag tacacgtttt tgcagcgtat cccgcgcagc cactacgtgc tggcagttgg
     3541 tcctgttcct tatctttatt atctccatca gatgcgattg ctggatatcg ccctgatcgc
     3601 ttttattgct atttccctcg cctttccggt gtttatctgg atgcgtccgc actggcagga
     3661 tatgttaaaa ctggaagcag cggcgcaacg atttggcgat gggcatctca atgagcgtat
     3721 ccactttgat gagggttcga gctttgaacg acttggtatc gcatttaacc agatggcgga
     3781 caatatcaac gccttgattg ccagcaaaaa acagcttatt gacggtatcg ctcacgaact
     3841 gcgaacaccg ttagtgcgcc tgcgttatcg actggagatg agcgataacc tgagcgccgc
     3901 cgaatcccag gcgttgaatc gtgatatcag tcaacttgaa gctttaattg aagagctgct
     3961 gacttatgcc cgactcgatc gcccacaaaa cgagcttcat cttagcgaac cagacctgcc
     4021 gttgtggctg tcaacgcatc tggcagatat tcaggcagta acgcccgata aaacggtacg
     4081 gataaaaacg ctcgtgcaag gccattatgc ggcgttggat atgcgcttaa tggagcgcgt
     4141 gctggataat ttgctcaata acgccctgcg gtactgccat tcaacggttg aaaccagcct
     4201 gctactgtcg gggaatagag cgacattaat tgttgaggat gatggcccag ggattgcccc
     4261 agaaaaccgc gaacatatct ttgaaccttt tgttcgcctc gatcccagcc gggatcgctc
     4321 aaccggcggc tgcgggctgg ggctggcaat tgtccactct atagcactgg caatgggcgg
     4381 tacggttaat tgtgacacca gcgaactggg tggtgctcgc ttctcgttta gctggccgtt
     4441 atggcataac atcccgcaat ttacctctgc ctgacactac gcgcacgatg gtcaagtcac
     4501 cacgactgtg ctataaaata agtatgttgt aactaaagtg gttaatatta tggcgcgtta
     4561 cgatctcgta gaccgactca acactacctt tcgccagatg gaacaagagc tggctacatt
     4621 tgccgctcat cttgagcaac acaagctatt ggttgcccgc gtgttctctt tgccggaggt
     4681 aaaaaaagag gatgagcata atccgcttaa tcgtattgag gtaaaacaac atctcggcaa
     4741 cgacgcgcag tcgctggcgt tgcgtcattt ccgcc
//
LOCUS       DAEVZA010000235         4740 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEVZA010000235 DAEVZA010000000
VERSION     DAEVZA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.235"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..242
                     /locus_tag="LAF92_004937"
     CDS             <1..242
                     /locus_tag="LAF92_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HBI9793823.1"
                     /translation="LSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFY
                     RNKQYHLPGDTADRLNYQKMAQVVDGVITLLYNSK"
     gene            345..848
                     /locus_tag="LAF92_004938"
     CDS             345..848
                     /locus_tag="LAF92_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBI9793824.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(915..2372)
                     /gene="nuoN"
                     /locus_tag="LAF92_004939"
     CDS             complement(915..2372)
                     /gene="nuoN"
                     /locus_tag="LAF92_004939"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBI9793825.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2379..3908)
                     /gene="nuoM"
                     /locus_tag="LAF92_004940"
     CDS             complement(2379..3908)
                     /gene="nuoM"
                     /locus_tag="LAF92_004940"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBI9793826.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4072..>4740)
                     /locus_tag="LAF92_004941"
     CDS             complement(4072..>4740)
                     /locus_tag="LAF92_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311189.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     ubiquinone; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBI9793827.1"
                     /translation="VTAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFI
                     VFHGKEQIHAHAVKGVTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTL
                     EITSGVVAVVGILLAAWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVK
                     PFLGIAWLLKRDPLNSMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALL
                     MVLR"
BASE COUNT         1300 a         1298 c         1239 g          903 t
ORIGIN      
        1 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
       61 ttgatttctc tgaccacctg aattattggc aacacgatat tcccgccgta atgattactg
      121 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
      181 attatcagaa aatggctcag gtggtggatg gtgttataac cttgttatac aacagtaaat
      241 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg cacattttat tttccctcgt
      301 ttattttaag atgcgaaaca tgaaattcaa cggaggaaaa ataaatggga atgattggct
      361 attttgcaga aattgactca gaaaaaatta accaactgct tgagtctact gaaaaaccct
      421 taatggacaa tattcatgac acgctttcag gattacgccg actggatatt gataaacgct
      481 gggatttttt acactttggg ctgacaggca cctctgcttt tgatcccgcg aagaatgatc
      541 cgctgagtcg tgccgtgctt ggtgaacaca gcctcgaaga tggtatagat ggtttcctgg
      601 gattgacatg gaatcaggag ttagccgcga caatcgaccg actggaagct ttggatcgaa
      661 gtgagctgcg taaaaagttt tccattaagc gactcaatga aatggagatt tatccgggag
      721 tgaccttttc agaagagtta gaggggcaac tctttgcttc gatcatgctt gatatggaaa
      781 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
      841 ttgggtgatg gtgtcagggc accaaaaaaa accgccgaac atgtcggcgg ctttctgact
      901 tacaaactga tagcttacat cagcggcatt gctaaccgca caatgctaat cagcggttgt
      961 ggccatacac ccagcaccag taccaacagt gcagagatca gcaccacgat accgcctgcg
     1021 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggggcgtg caggtacagg
     1081 ctcaccgcca cgcgcaggta gtagtaaagg ccgattgccg aaccgacaac cacggcgccc
     1141 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accaataaag
     1201 cccagcgtca tcggaatacc ggccagagac agcatcatca ccgtcatcac cgccgcgagg
     1261 atcgggcgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacgg
     1321 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     1381 gccaggtaaa ctcctaccgc ttccatcgac atctcgcccg tttgcagcgc aatcagcgcc
     1441 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatattggtc
     1501 tggctcagcg ccatcaggtt accgaagatg atagaggcaa aggcgataat cgccagcact
     1561 acccgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     1621 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     1681 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc aaggccaaca
     1741 atcatcaggc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     1801 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     1861 agcaggaaag aagacgctgc ggcagaaagg atagtgtatt tgatactggc ttccagcgaa
     1921 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcgata
     1981 ccgaggaaca gagacgccag atggttggca ttcgccagca ggatcccgcc cagcgcggca
     2041 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2101 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     2161 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     2221 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     2281 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     2341 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     2401 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     2461 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     2521 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     2581 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     2641 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     2701 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     2761 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     2821 taaagctgac cacaaagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     2881 attaccgcgc cctggtaggc caactggctg ccggtgtaga tagcaatcag cacgaagccc
     2941 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgggc gaaagccatc
     3001 cacgcaccgt agaagatgcc gataaccccc agccacatag cgattggcgc aaactctgcc
     3061 gatgcgttcg ggaacagcgg cagggagaaa cgcagcaaac cgtaagcggc agttttcagc
     3121 aagatccccg cgaggtcaac ggaaccggcg gtcggagcct gagagtgcgc atccggtagc
     3181 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatt
     3241 aacaggtatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     3301 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcaat caacatcacc
     3361 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     3421 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     3481 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     3541 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacag
     3601 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagcccgtca
     3661 atggcgagat ggatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     3721 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     3781 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgcggcac cttgacgcca
     3841 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     3901 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     3961 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4021 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4081 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4141 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     4201 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     4261 cttgacgaac actttgtcgt acagccagtc aaagccccag gcgttgtacc accaggtact
     4321 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagtacgtt tacccagcca
     4381 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     4441 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     4501 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     4561 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     4621 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     4681 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
//
LOCUS       DAEVZA010000236         4686 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEVZA010000236 DAEVZA010000000
VERSION     DAEVZA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.236"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..957)
                     /gene="glpC"
                     /locus_tag="LAF92_004942"
     CDS             complement(<1..957)
                     /gene="glpC"
                     /locus_tag="LAF92_004942"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBI9793828.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPG"
     gene            complement(954..2213)
                     /gene="glpB"
                     /locus_tag="LAF92_004943"
     CDS             complement(954..2213)
                     /gene="glpB"
                     /locus_tag="LAF92_004943"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBI9793829.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(2203..3831)
                     /gene="glpA"
                     /locus_tag="LAF92_004944"
     CDS             complement(2203..3831)
                     /gene="glpA"
                     /locus_tag="LAF92_004944"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBI9793830.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4104..>4686
                     /locus_tag="LAF92_004945"
     CDS             4104..>4686
                     /locus_tag="LAF92_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9793831.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPA"
BASE COUNT         1006 a         1265 c         1294 g         1121 t
ORIGIN      
        1 ccccgggatt ttacgcaaca gctccagggt gtagagcgtc cagcccattt tttccatatg
       61 gcacggagtg tgataaacca ctttcagcgg cagcggtttc agcggtaacg ttttgccttc
      121 gtccagcttg cgccacagcc agcgggttgc cagttcgata tgatcgcgca agcctttgtt
      181 gtcgacattc agcacttccg ggtattcgtc gcgcagggca aatgtacagg ttgaggaggt
      241 ggcaatcacc ggaatgcctt ttactcccac agcttcgcgg atcgactcta cattcgtaat
      301 tgcctgtttg cgtgctttat cggtaaagcc gttggcgatt agcggtacgc cgcagcattt
      361 ttctttgctg agcagttgta caccggtacc cattgcgttg agcactttaa ttaaatcttt
      421 acctaactgc ggatggttgt agttaacgaa gcagccgtga aagaaagcga cctggtcttt
      481 atattgtgct tgctgagccg ctatgctgcg ataccagcga cggaacgtgc cgaaggagta
      541 tttcggtagc gtgcggcgat gatcgatttt taacgccgca tcaagcagct gccgcaccgg
      601 tttcagcgag gtagcggtgt tgacgattgg tgcgaacggc gtggaaacgc tacccatcag
      661 gtcggtatga ctcaacacaa aattacgcag cgacgggcgc gtggtgtcat attttgcccg
      721 cgcgcgctgg ataatatcgc caatcttcac atcggacggg caggcgactt cacaacgttt
      781 gcagttgatg caatatttca gcgcctcgtc atacagtgcg ccatctttca aacgcagacg
      841 ctcgccatcc ggcccggctt gttttggccc tggataaccg ggattcaccc ggctcaccgg
      901 gcaggcggtg gtgcagacgg tgcacttaat gcagttttcg aagctggtgt cgttcattgt
      961 tggcctcctg cgcgttgggc aatctgttga gcggcatgta aagcactgac ggcacaaaca
     1021 ccgccgccgc atccctgggc gatgggatca aatccgccca gcaccgaacc gatggcaaac
     1081 aggttctcaa tggtttgccc tgcctgcgac gggcgtagcg tctcatcagt ggttacaccg
     1141 aactgctgcc acggttgcgg cgcaaaaaaa tcgcccttat accattcacc ccgcgtggcg
     1201 gtttgtagca catcaaggcc gagaatcggc tcgcgaatgc cgttacgttc cgctaccagt
     1261 ccgccactaa agaaactgcc gctggcgaga accgcgaaac gtggacgtag cggaatatcg
     1321 gcgtgattgc gggtccagat ttcattcact acgccatttt tacaggtcac ttttttcact
     1381 tcatcgcccg gcatccacac gccaccctgg cgcacaaact ggcgctgtaa ctggttttgc
     1441 agacgaatgc ccagcacgga aggcggcagc gttggcaaaa gcatcagtga acaaggtagt
     1501 ttttcattca accaacgcca cagtttgtca tcggccaggc cgaagcaggc gggcatcagg
     1561 atcatttcgc aggtattggc gacaggaata agcgcatcaa gtaacagcgg ccagttttct
     1621 tcattatcaa ggaaacgggc gatattcaca gcgcgaaatt cggtggcgtt attgcgcagg
     1681 acatccagtt ccggcagctc tatttctgcg gtttcaaccg aaagatccag ttcgcgcaac
     1741 gaagccgccg caagatgcgc ctggaaatcc atcagcccgc ttatccccac tacacatatc
     1801 ttcttcgcgg gtagcggcca gacgggaact tctggcgaac ttaaccaggt tgcacgcaaa
     1861 gtgccgagcg gggtaacgcg ctgatgggcc agttccacgc tgccctgcaa ttgcgcaccg
     1921 ctttcagcga tcaatgcctg cgcctggcaa gcgagatcga gcacgcgttg tggttcgaga
     1981 agactgtaag gatgggctgg tgcctgctga cgcaaagatt ccagtccact gtgaatgtct
     2041 gtcaccggtt gaccatcagg cagatggctc agcaaatcca gcgaaccgga tgagaaatgc
     2101 agcgcgcttt gaccacgcgt gacaatggca cagcgcaggc cgtgtttttg cagttgcagg
     2161 ccacagagta atccggcgag gccgccgccc ataatgacag tatcaaagcg catctttctg
     2221 ctccttctcc agaccacaca gtccctgata aacccagcgg gtaaattcgc tttcgcgcag
     2281 tgcgtctccc caggcgatgg gttgcacgcc tttccagcgt tcgttaagga aggtggaaag
     2341 ttgctcgata gattgcgcgg acgtcgtgac gttaaaacgt tgcagcagtc cggcagcgcg
     2401 gcaggcgcag agttcgccct ggcaggtacc catccccaca cgggtacgac ggcgtaaatc
     2461 cagcaggcta ttaacgttta aattttctac cgcgtactga acttcacccg cagttaccgc
     2521 ttcgcactca cataccaggc tacgatgcag acggccttcg ctcagccagg caggcgtgcg
     2581 atcgccatga cgataaaccg cagaaccgcg cagcggggca ggcagggaga tgactttacg
     2641 caaggtaact tcagcgggtt cttgtgaacc aggcagtgcc agatcggcag tcgtacaggg
     2701 gcgcgtgttg cccagtttgc ggcataccgc gtcggtagcc cattcagcca tcagccgata
     2761 ggtcatcagt ttgccaccgg tgatggtgat aaatccgtcc agaccatcgc gttcagcatg
     2821 gtcgagcagc acgatgccac ggctgacgtt acgtccgctc gggtcgtcat cgctggcaac
     2881 cagcgggcgc acgccagaat aggcccgcaa aatgcgcgtt ttcgccatca cgggggccag
     2941 tttttcccct tcacgcagca gaatatcaac ctcttctgcc gtcactcgat tatcgtcaat
     3001 ctcgttgtaa tcaatacgta aagaggtggt gccaatcagc gaaatggtat cgccaggcac
     3061 cagaatatcg gcgtcggaag gtttacggca gcggttgatc acatgctggt taatgcggtg
     3121 atccatgatc agcagcgatc ctttcgccgg gaacatgcga atgcgcagat cggcatattc
     3181 ggcaatgtgt tgcccccaga tcccagcggc attaaccacg acaggtgcat gaagggcctg
     3241 agtttcgccg gtgagatggt tacgtacacg aacaccgcac accgtcgcgc cttcacgaat
     3301 cagccccgtg acttcatgag cggtaaggat aacggcaccg tgttctttgg catccagcat
     3361 gtttgctgcg gtcagacgaa atggatcaac ggtgccatcc ggaactttca ccgcgccaat
     3421 cagtgccggg ttaacggcag gttcgataat gcgcgcttgc tgcgggtcta tagcttctgc
     3481 gctgatccct gcttcttcgc aggcgcgaat aaaagtggcc tggaatgaga ggtcatcttc
     3541 cggcagggtg ataaacaggc cgttggttgg ttcaacgcag tgacgtgcaa tgcgtttcag
     3601 gatctggttt tcactaatgc attcgcgggc cgattccgca tcggttaccg catagcgcgc
     3661 accgctgtgc agcaggccgt ggttacgccc ggtggcaccg gttgcgatgt cgtggcgctc
     3721 aaccaaaatc acgcgcagcc cgcgcagggc acagtcgcgg gcaatcccgg ctcccgttgc
     3781 gccgccacca atgataatca cgtcacttga ttgcgagtcg cgagttttca ttgttatccc
     3841 tctgaagttc gttttttacc atttagccat agtaaaaaca tgaattgttt gatttcgcgc
     3901 atattcgctc ataattcgaa agtgaaacgt gatttcatgc gtcattctga acattttgta
     3961 aatcttattt aataatgtgt gcggcaattc acatttaatt tctgaatgtt ttcttaacat
     4021 cgcggcaact aaagaaacgg caggttctcc cactgaatca gtctgttaat cataaataag
     4081 accacgggcc acggaggctt tcaatgttga gtatttttaa accagcgcca cacaaagcgc
     4141 gcttacctgc tgcggagatc gatccgactt atcgtcgatt gcgctggcaa attttcctgg
     4201 ggatattctt tggctatgcg gcttactatt tggttcgtaa gaactttgcg cttgctatgc
     4261 cttatctggt cgagcaggga ttctctcgcg gtgatttagg tttcgcgctt tcggggatct
     4321 cgatcgctta tggattttcg aaattcataa tgggttcggt atcagatcgc tcgaatccgc
     4381 gcgttttcct ccccgcaggt ttgattctgg cggcggcagt gatgttgttt atgggctttg
     4441 tgccatgggc gacgtcgagc attgcggtga tgtttgtact tttgttcctc tgcggttggt
     4501 tccaggggat ggggtggccg ccgtgtggtc gtactatggt gcactggtgg tcgcagaaag
     4561 aacgtggcgg cattgtgtca gtgtggaact gtgcgcacaa cgttggtggt ggtattccgc
     4621 cgctgctgtt cctgctgggg atggcctggt tcaatgactg gcacgcggcg ctctatatgc
     4681 ccgctt
//
LOCUS       DAEVZA010000237         4661 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEVZA010000237 DAEVZA010000000
VERSION     DAEVZA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.237"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..539)
                     /locus_tag="LAF92_004946"
     CDS             complement(<1..539)
                     /locus_tag="LAF92_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBI9793832.1"
                     /translation="MQDIQQETLNECIRAEQSASVVLWEIDLTEVGGDRYFFCNEQNE
                     KGESVTWQSRQYQAYPIQGSGFEMNGKGSAARPTLTVSNLHGMITGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSEADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRG"
     gene            complement(539..877)
                     /locus_tag="LAF92_004947"
     CDS             complement(539..877)
                     /locus_tag="LAF92_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9793833.1"
                     /translation="MANTEEFHWLPEDGMKTENKPSIKTVRFGDGYEQRSPNGLNHSL
                     RVFTCDFKVEANERDSFEKFLARHEGYKSFFWRPPGINRKIRVVCRTWSATEHITYTD
                     FSCQFDEVVI"
     gene            complement(870..4103)
                     /locus_tag="LAF92_004948"
     CDS             complement(870..4103)
                     /locus_tag="LAF92_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBI9793834.1"
                     /translation="MAEQTSRLAIIIDSTGAKNNADNLTSSLVKMTQAGETAANSAGK
                     VTKATEDEKNALAKLKAAIDPVGAAIDTVGRRYSELKKFFDKGLIDKEEYEFLVRKLN
                     ETTEELSGVAQAQREAEKAGKLAAAQQEAQAQAFQRMLDKIDPLAAALRNLEQQHDEL
                     NAAFASGKINGSQFENYSRKIQETRRELTGEAQAEREAAKAHDEQVVALQRLIAQLDP
                     VGTAFNRLVEQQKQLNEAKAKGMLSPEMYEELSGKLRAMRSELEVTQSQLSKTGMSAK
                     QTAFAMRMLPAQMTDIVVGLSTGQSPFMVLMQQGGQLKDMFGGIGPAIKGVGSYVLGL
                     INPFTLAAAAVGVLGLAYYKGSQEQDEFNKSLILTGNQLGTTSGQLGDIAQRAGNAAD
                     STTGAAAAVLNQLVRSGKVASSSLEQVTTAIVKTSEVTGISTEQLVNDFNEIAKDPVS
                     AISKLNDQYHFLTLATYNQIKALQDEGNQHEAARIATEEYSSSMIQRTNQIKENLGYL
                     ETAWKAVADSAKWAWDSMLDIGREASLDQKISDVLRQIDEIEKNTRPGVFGLGGIGDG
                     GAQNKRLARLKQQLGVLQAEKIAQDVLNSSINDYNKRQQEGIELRQRADAFSKQYQTR
                     EQQRASELAKLEKLKNQYSKEEYNNLIAQINERYKDPKQPKAKGYSDDAAQRMIDHLN
                     QQNALLSSQAELTVKLSSSEQELVKWRQRIADLESRPSSKLTQDQKSLLLHREEITAL
                     MEKNVAIEKNNRLIKESAEITAWRDSLQASIDNRQQGYDIQIAGYGVGDKNQQRQQEL
                     LRIEHGYNNQRLQLERDYADKSRGMSDHVFQEKMQALNDALEREKEIVRQKNEQLDIQ
                     AGDWVSGASQGFNNWLDDTKDISEQIKSTTTQMFDGMTDALGDFVTTGKANFRSFATS
                     VISDLSRMALKASITGIFDSISNSSSGGILGTIGSAISKFIPNAKGGVYESPSLSTYS
                     NGIYDSPQFFAFAKGAGVFGEAGPEAIMPLTRTSDGSLGVRAINSKSGNGGGDITYAP
                     VYQITIQNDGQNGEIGPQAIKALMGMVDQRVQGNLLNMRRDGGILSG"
     gene            complement(4269..4469)
                     /locus_tag="LAF92_004949"
     CDS             complement(4269..4469)
                     /locus_tag="LAF92_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793835.1"
                     /translation="MLKELLYAYSVISRARRYAGMAGVPLPLSLTEINEYLATHPVLI
                     ERDEFEAVIFALDDQYFQEQCV"
     gene            complement(4577..>4661)
                     /locus_tag="LAF92_004950"
     CDS             complement(4577..>4661)
                     /locus_tag="LAF92_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBI9793836.1"
                     /translation="LYWQILAEAASIAQGKEQQKQDTIKKP"
BASE COUNT         1121 a         1234 c          929 g         1377 t
ORIGIN      
        1 tcaccgcgat aggtccaggt gcaggtgttg gccagcatga tacgtcccgg aaaaacggca
       61 ccgtccgttt ccgtcggcgt ggacagtaca aaagaggcac tgaccgcgct cagttcgctg
      121 cactgctcga tgcgccagcg gctgatcacc tcctgctccg gatcggcttc gctgtttccg
      181 ttgacgaagt tcaccgcatc cagaaagcgg gcgtaaacct tacgccggac caccgttccg
      241 ccgaccagac tctgcagatc ttccgccatc ccggtgatca tgccgtgcag gttagagacc
      301 gtcagtgtcg gacgggcagc actgcccttg ccgttcatct caaatccact cccctgaatg
      361 ggataggcct gatactgccg actctgccag gtgacagact cacctttttc gttctgctca
      421 ttacagaaaa agtagcgatc cccgcctacc tcggtcaggt cgatttccca gagcaccacg
      481 ctggccgact gctccgcacg aatgcattca ttcagcgttt cctgttgtat gtcctgcatc
      541 agatcaccac ttcgtcaaac tgacacgaaa aatcggtata ggtgatatgt tctgtcgctg
      601 accacgttcg acacaccact ctgatttttc tgttaatacc cggcgggcgc caaaaaaaag
      661 atttatagcc ttcatgccgg gctaaaaatt tttcaaatga atcacgttca ttcgcctcaa
      721 ctttgaaatc acaggtgaaa acgcgcagag aatgattaag tccatttgga cttcgttgct
      781 cataaccatc gccaaatctt acagttttta tcgatggttt attttctgtt ttcattccat
      841 cctctggtaa ccagtgaaat tcttccgtat tagccactta atattccccc atcacgtcgc
      901 atatttaaca gattgccctg cacccgctga tcaaccatcc ccataagtgc ttttattgcc
      961 tgagggccaa tctctccatt ctggccgtca ttttgaatag tgatttggta tacaggggca
     1021 taggtaatat ctccgcctcc attaccactt ttactattaa tagctctgac accaagagaa
     1081 ccatcggaag ttcgtgttaa tggcataatg gcttccggtc cagcctcacc aaaaacacca
     1141 gccccttttg caaaagcaaa aaattgcggg gaatcataaa taccgttcga atacgtgctc
     1201 aatgacggag actcataaac accgcccttt gcattcggaa taaatttact aatagcactc
     1261 ccgatagttc ctaaaatccc cccagaagaa ctgttactaa tgctgtcgaa aatcccagta
     1321 attgaagcct ttaatgccat tcgactaaga tccgaaatca cggaagtagc gaaagaacga
     1381 aaatttgcct tgcctgtcgt gacaaaatca cccagcgcat cggtcatccc atcaaacatc
     1441 tgagtcgtgg ttgattttat ctgctcactg atatccttag tgtcatccag ccagttattg
     1501 aatccctggg aggcaccact aacccagtct cctgcctgaa tatcgagctg ctcgtttttc
     1561 tgtctgacaa tttctttttc ccgttccaga gcatcattca gagcctgcat tttctcttga
     1621 aaaacatgat ctgacattcc acgggattta tctgcatagt cacgttcaag ttgcagacgc
     1681 tgattgttat atccatgttc aatccgcagt aattcctgct ggcgttgctg atttttatcg
     1741 ccaaccccat aaccagcaat ctgaatatca tacccctgct gacgattatc aatcgaagcc
     1801 tgcaatgaat cacgccatgc tgttatttcg gcggattcct tgattagcct gttattcttt
     1861 tcaatcgcaa catttttctc catcaacgcg gttatttctt cccggtgtaa gagaagcgat
     1921 ttctgatcct gggttaattt cgatgatggt cgtgactcta ggtcggcaat ccgctgacgc
     1981 cacttaacca gttcctgttc agaggaactt aatttaacgg tcaactcagc ttgtgaactt
     2041 agtaacgcat tctgttgatt cagatgatca atcattcgtt gggctgcatc atcagaataa
     2101 cctttcgcct ttggttgctt tggatcttta taacgctcat ttatttgagc gataagatta
     2161 ttatattctt cctttgaata ctgattcttt agcttttcca gttttgcaag ttcactagct
     2221 ctctgctgct cccgggtctg atattgtttt gaaaaggcat ctgctctctg tctgagttca
     2281 attccttcct gttgtcgctt gttgtaatcg tttattgatg aatttagtac gtcctgagca
     2341 attttttctg cttgaagtac gcccaattgc tgctttaatc gtgccagcct tttattttga
     2401 gctccgccat ctccaatgcc acctaacccg aaaactccgg gtcgggtatt tttttctatt
     2461 tcatcaattt gacggagaac atctgagatt ttttgatcaa gggaggcctc acggccaata
     2521 tccagcatgg aatcccatgc ccattttgcg gagtctgcga cagctttcca tgcagtctca
     2581 agataaccaa gattttcttt aatctggttg gtgcgctgga tcattgagga tgagtattct
     2641 tctgtcgcaa tgcgggcggc ctcatgctgg ttcccttcat cctgtagcgc cttaatctgg
     2701 ttataagtcg caagtgtcag aaaatggtac tgatcattaa gttttgatat ggcactgaca
     2761 ggatcctttg caatttcatt gaagtcatta accagttgtt cggttgatat tcctgttact
     2821 tcgctcgttt ttactatcgc tgtcgtcact tgctccagcg aactgctcgc tacctttccc
     2881 gaacgcacaa gctgatttaa tactgccgcc gcagcaccag ttgtcgaatc agccgcattc
     2941 ccggcacgtt gagctatatc gcccaattgc ccgctggttg tccccaactg atttccggta
     3001 agaataagag atttattaaa ttcgtcctgc tcctgagagc ctttatagta agccagtcct
     3061 aagaccccaa cggctgctgc ggccagggta aaaggattaa ttaatcccag cacataagaa
     3121 cctacacctt tgatcgccgg gccaatccca ccgaacatat cttttagctg gccgccctgc
     3181 tgcattaaca ccataaatgg cgactgacca gtggacaacc caacaacaat atccgtcatt
     3241 tgtgcaggca acatgcgcat agcaaaagcc gtttgttttg ccgacattcc ggttttgctt
     3301 aattgtgatt gagtaacctc aagctcactc cgcatagcac gaagttttcc agaaagctcc
     3361 tcatacattt caggagaaag catcccctta gcttttgctt cattgagctg tttctgttgt
     3421 tctaccagac gattaaaagc agttccgaca ggatcaagtt gagcaatcag acgttgcaaa
     3481 gcaacaacct gttcatcatg cgcttttgct gcttctcgct ctgcctgagc ctctccggta
     3541 agctctcgcc gtgtttcctg tatttttcgg ctataattct caaactgaga accatttatt
     3601 ttcccggatg caaacgcagc attaagttca tcatgctgtt gttcaagatt tcttagcgcc
     3661 gcagccagag ggtcgatctt gtccagcatt ctttgaaagg cctgagcctg cgcttcctgc
     3721 tgggcggcag caagttttcc ggccttctcg gcttctctct gcgcttgcgc aaccccgctc
     3781 aattcctctg tggtttcatt aagtttacgg acaagaaatt catattcttc tttatcaata
     3841 agccctttat cgaaaaattt ctttaattca gaatagcgtc gaccgacagt atcaattgcg
     3901 gcaccaactg gatcaatagc tgcttttaat tttgcgagcg cgttcttctc atcttctgtt
     3961 gccttagtca ctttccctgc gctatttgca gcagtttccc cagcctgcgt cattttgact
     4021 aatgaggagg tcagattgtc agcattattt ttcgctccag tgctatcaat aattattgcg
     4081 agacgcgagg tttgctctgc catttattaa aactcctgac aacaaaaaac ccaccgcgaa
     4141 gtgggtttca ggcgacataa tagtagatat agcgattacg aggccacgca atgcttttct
     4201 ccaggagcat catcgattta attaaagaca ccatcacatc tctgtaacag agtgtacgta
     4261 attaacaact acacacactg ctcctgaaaa tactggtcat ccagtgcaaa gatcactgct
     4321 tcaaattcat cgcgctcaat caataccgga tgagtggcta aatattcatt tatctctgtc
     4381 agagataaag gcaaaggcac cccagccatt ccagcataac gtcgggcacg ggatattacc
     4441 gaataggcgt acaacaactc cttaagcacc gggtctattt ctggttccgg tatcggtggc
     4501 aacctgagtt tttctcgctt ccatcttgcc ttttcccccc tttctccccc gaactccgat
     4561 aaccaccgct gggcagctat ggctttttta tcgtatcctg cttctgctgc tctttaccct
     4621 gggcgatgct ggctgcttct gcaaggatct gccagtacaa c
//
LOCUS       DAEVZA010000238         4609 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEVZA010000238 DAEVZA010000000
VERSION     DAEVZA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.238"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..498)
                     /locus_tag="LAF92_004951"
     CDS             complement(<1..498)
                     /locus_tag="LAF92_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBI9793837.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAI"
     gene            complement(575..868)
                     /locus_tag="LAF92_004952"
     CDS             complement(575..868)
                     /locus_tag="LAF92_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793838.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            1015..1977
                     /gene="parM/stbA"
                     /locus_tag="LAF92_004953"
     CDS             1015..1977
                     /gene="parM/stbA"
                     /locus_tag="LAF92_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBI9793839.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            1980..2330
                     /locus_tag="LAF92_004954"
     CDS             1980..2330
                     /locus_tag="LAF92_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBI9793840.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(2453..2734)
                     /locus_tag="LAF92_004955"
     CDS             complement(2453..2734)
                     /locus_tag="LAF92_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793841.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(2862..3096)
                     /locus_tag="LAF92_004956"
                     /pseudo
     CDS             complement(2862..3096)
                     /locus_tag="LAF92_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4545..>4609)
                     /locus_tag="LAF92_004957"
     CDS             complement(4545..>4609)
                     /locus_tag="LAF92_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793842.1"
                     /translation="KSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1374 a         1020 c          940 g         1275 t
ORIGIN      
        1 aattgcgttc gggtgtgtga agctctcaat cagcggttgc aggctggtaa cgggcttttc
       61 cgtcgggtga tggcgattgc ccgaatattt ccagcccagc acgtccggca gcgggttttg
      121 tggcagacgt ggacggcctt ttgccaggat gtaggcacat tcgtggcgat agcccacata
      181 tgcggccttc gatgtgtagt ttttggtgaa gaccaggtga ccaacaacgc tgaatcccgc
      241 atttttccag gcggccataa agcgatcgac gcggttccag ccatagaagc tcaccattaa
      301 cgcgtctttt ttgagtacgc ggtacatttc attacaggct ggttgcagcc attcatcggt
      361 tttatcgccg gcgatggtgc gcccggaacg gtcacggaaa ccgacgagat acggcgggtc
      421 ggtgaggatg aaatcgacag cattgcccgg aaacgtggcc ataacacgca cgcagtcacc
      481 caggataaaa cgggacataa cagcactcct tctcttacag agggcaccgg gagggcaccc
      541 ctgtggtgcc cgttccccgc tgcctgtggg ctgttcaccg cgtccggtaa cgggtcaggc
      601 cgaccgtgga ataccgcagc cgtcgtcttt ttgacggcga ggatatgccg cgaaagcggc
      661 ttgaagggcg ttcatcagaa aagggcgacg ggctggcgta cactgtcacc aacgccagcc
      721 gggagcggta aaagcgcccg tcccgtgccc ggccggacgc gggacactga ccacaggcag
      781 cgttcctgcc gcgccgcgtc agccggcgca gtaaaaagag gagcccggcg acatcttttg
      841 ttacccgcca aacaaaaccc aaaaacaacc catacccaac ccaataaaac accaaaacaa
      901 gacaaataat cattgattga tggttgaaat ggggtaaact tgacaaacaa acccacttaa
      961 aacccaaaac atacccaaac acacaccaaa aaaacaccat aaggagtttt ataaatgttg
     1021 gtattcattg atgacggttc aacaaacatc aaactacagt ggcaggaaag cgacggaaca
     1081 attaaacagc acattagccc gaacagcttc aaacgcgagt gggcagtctc ttttggtgat
     1141 aaaaaggtct ttaactacac actgaacggc gaacagtatt catttgatcc aatcagcccg
     1201 gatgctgtag tcacaaccaa tatcgcatgg caatacagcg acgttaatgt cgttgcagtg
     1261 catcacgcct tactgaccag tggtctgccg gtaagcgaag tggatattgt ttgcacactt
     1321 cctctgacag agtattacga cagaaataac caacccaata cggaaaatat tgagcgtaag
     1381 aaagcaaact tccggaaaaa aattacatta aatggcgggg atacattcac aataaaagat
     1441 gtaaaagtca tgcctgaatc tataccggca ggttatgaag ttctacaaga actggatgag
     1501 ttagattctt tattaattat agatctcggg ggcaccacat tagatatttc tcaggtaatg
     1561 gggaaattat cggggatcag taaaatatac ggagactcat ctcttggtgt ctctctggtt
     1621 acatctgcag taaaagatgc cctttctctt gcgagaacaa aaggaagtag ctatcttgct
     1681 gacgatataa tcattcacag aaaagataat aactatctga agcaacgaat taatgatgag
     1741 aacaaaatat caatagtcac cgaagcaatg aatgaagcac ttcgtaaact tgagcaacgt
     1801 gtattaaata cgctcaatga attttctggt tatactcatg ttatggttat aggcggtggc
     1861 gcagaattaa tatgcgatgc agtaaaaaaa cacacacaga ttcgtgatga acgttttttc
     1921 aaaaccaata actctcaata tgatttagtt aacggtatgt atctcatagg taattaatga
     1981 tggacaagcg cagaaccatt gccttcaaac taaatccaga tgtaaatcaa acagataaaa
     2041 ttgtttgtga tacactggac agtatcccgc aaggggaacg aagccgcctt aaccgggccg
     2101 cactgacggc aggtctggcc ttatacagac aagatccccg gacccctttc cttttatgtg
     2161 agctgctgac gaaagaaacc acattttcag atatcgtgaa tatattgaga tcgctatttc
     2221 caaaagagat ggccgatttt aattcttcaa tagtcactca atcctcttca caacaagagc
     2281 aaaaaagtga tgaagagacc aaaaaaaatg cgatgaagct aataaattaa ttcaattatt
     2341 attgagttcc ctttatccac tatcaggctg gataaaggga actcaatcaa gttattttct
     2401 taccagtcat tacataatcg ttattatgaa ataatcgttt gcactgtctc tgttattcag
     2461 gcaatttcaa taaaggcact tgctcacgct ctgtcatttt ctgaaactct tcatgctgca
     2521 tttcgatttt tcttctaaaa tcataacttg catacctgtt ctttttatgt atatccagat
     2581 ctatttttgt gacgagttca aaccacaatg atagcactcc ccatgcagcg ttatgaaaca
     2641 tttccgcaac agatttagag tctgtattaa ccgcttcagt catgtagtcg ctaatacgtt
     2701 ctgcagtgat tttcgttatt tcatcgtaat tcattttatc ccctacaaga ctatttaatg
     2761 gaatgaaaaa tatttggtct gataacaatg ctcctgaatt aacacctgca ggctatgtta
     2821 aagttgccct cacacacaat ctacctgttt tctcgtgtgt gtcacaggcg caatacataa
     2881 atagtctggc cattaagttc gtaacggatt tcgtactcgc cagcaaaaat ccgtctgaca
     2941 tccctggttt caaactgaaa caactgttca tcccatacgt ggatgagtta acagaatgac
     3001 cggcgcctgt gtcagggact acaccgtttt ggctgcagct ggtttactga ccaacgccag
     3061 aaaatcatat aactgcgtca atgtcagcgt atccagcgcg actgtattat ttttctctgc
     3121 aaccagaata ttgcattagg ttattaccag gtgttctaat gtacaggtaa agacgttctt
     3181 tataatctga aatctctact tttaatccct cagactttgc ctgctgaaag ccctcgtcaa
     3241 ggtgcgcttt tattaggtta atgtctggat gtcttgtatc gggggcaatt ctgtattggt
     3301 gtaacgatgg ggtggatgtt ccctgcaata agacctccag atagagattg gcacaaccat
     3361 tcccattatt tctaatagtt tattggtaat cacaaactgt atatttagcc attatattga
     3421 atcctgtgaa gtaaacggcg acggctcaaa attttatcaa acctaagata attctaccat
     3481 caaagttaat aatttgaatt tatatgacgg ctctatgggg gggtaaaaat ccccgtgcag
     3541 agaggcgatt ttcagagttg aaaacgtcgt cgttaagaac accaaagtca gtaccaccaa
     3601 cccggaaagt agcttcatgc accgggacaa cagcagtttt ggtgtccgaa atcgtacaat
     3661 tctgtacatc tacgcaagga aatgtacgga agtaagccaa atggttatcg gacggtcgga
     3721 acatttcgta cagcctttac gttcagtgag ttagctggcc tagatgtaac cactcgttca
     3781 actcaattct cttagcgtgg gaaaatttcc gttttccaag cggccccctc aagcggaccc
     3841 cttatagtac aataattttc gatatccaaa ctgcccccaa ttacatctcc agtctctggc
     3901 agcagagatc atatatttcc attgccgccg tagccacgat attctggcta ttcacaaaaa
     3961 caatttagcc ccaaagcgca gtaaaaaagt acactatgaa ggtgtatgat accttcatag
     4021 tgcttaagta attatgagta atataatctg tgtaaactac taattgcttc accgtcagtg
     4081 attaaatgcg tagctgttcc ttgtcttata taatacctat tttcaaaatg acagacttct
     4141 ccgacacttt ttatttcgat ttttaaaaca tgcagtcctt catatgaaca taatgaaata
     4201 tcttttagta tttgttgttt caaagcctca ttaaaattaa atgattctat tttttgtttt
     4261 atgaacagaa agtaattatc tatgtttttt gattgaatta cagcttcatg atcaattcca
     4321 ttgatataat agccattgca ctcaataggg gtaacaccat ataaagtctt aactctgtct
     4381 gccgtagctt tggtatcgga aacaccaact aaaatataac cattagattc tctaccgatg
     4441 ttatttatgg cgacagccgt ttggactata ttaatgaaac attcttcgtc tattttattc
     4501 tcgcctgaga gaataaataa tccctgtttg gtaatgactc caacttattg atagtgtttt
     4561 atgttcagat aatgcccgat gactttgtca tgcagctcca ccgattttg
//
LOCUS       DAEVZA010000239         4550 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEVZA010000239 DAEVZA010000000
VERSION     DAEVZA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.239"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(83..880)
                     /gene="mtfA"
                     /locus_tag="LAF92_004958"
     CDS             complement(83..880)
                     /gene="mtfA"
                     /locus_tag="LAF92_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBI9793843.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            974..1063
                     /locus_tag="LAF92_004959"
     tRNA            974..1063
                     /locus_tag="LAF92_004959"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1008..1010,aa:Ser,seq:cga)
     gene            complement(1633..2163)
                     /locus_tag="LAF92_004960"
     CDS             complement(1633..2163)
                     /locus_tag="LAF92_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBI9793844.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(2506..3156)
                     /gene="zinT"
                     /locus_tag="LAF92_004961"
     CDS             complement(2506..3156)
                     /gene="zinT"
                     /locus_tag="LAF92_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBI9793845.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(3413..4048)
                     /gene="msrQ"
                     /locus_tag="LAF92_004962"
     CDS             complement(3413..4048)
                     /gene="msrQ"
                     /locus_tag="LAF92_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBI9793846.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(4049..>4550)
                     /gene="msrP"
                     /locus_tag="LAF92_004963"
     CDS             complement(4049..>4550)
                     /gene="msrP"
                     /locus_tag="LAF92_004963"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBI9793847.1"
                     /translation="EPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDE
                     AMHPLTLMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLA
                     APDEYGFYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGL
                     DLRENF"
BASE COUNT         1288 a          984 c         1015 g         1263 t
ORIGIN      
        1 atattactta gcggtacctt gtctgtcaac actaaatttc ataagtcatt tcaattggtt
       61 aattaatctg caaagttgtt aattaatgaa cattcgtcgc cgaaaacgag tctgtatcat
      121 tagcgtgatg cagtctctgc aaaggatctt gttgataaaa ttggcagaaa cgttgccaca
      181 atgaagggaa acgaggagca aaaagttctg gggcgctaaa gaaatattca gaaagtacgg
      241 caaaacattc agcaggatcg ctggctgcat aagcatcaat gctcgccgca ttctcgccaa
      301 ccaattcgat ttcttcctga atgttgttca ttgcagcatg aagatcgtgt tcccagccag
      361 caacctcacg caacggaata aagggaactc cgctggcgcg atcgccgtta cgggtgtcca
      421 gcttatgagc gacttcatga ataatcaggt taaaaccaga agcatcaaaa gaatcttgta
      481 tatccaacca gttcaaaacg ataggccctt gctgccagct ctgacctgac tgaacaatac
      541 gttggttatg caccagaccg atatcgtctt cccattcatc atcgaccaca aatggcgcag
      601 gataaattaa gacttcatga aaaccatcca gccattccag tcctaactcc agaacgggta
      661 ggcaaaatag aagtgctatc cggcagcttc ttaatgaatc cagttcaaag ccctgtaaag
      721 gaacaagccg cttttgctgt aaaaaacgtt cggcaagagt gactaatttg ctttgttcct
      781 gttccgtcag acacgttaaa agggggatcg atagtgcttc ctgccaggga agggcagttt
      841 gatgtgctga ttcttgtact ttccagggcc acttaatcat cgttttgctc gcaaactcgt
      901 cacttgaaca aaattgcacg gacagggact gttaaaatgc caaatttcct ggcatcatgg
      961 caaccatctg aacggagaga tgccggagcg gctgaacgga ccggtctcga aaaccggagt
     1021 aggggcaact ctaccggggg ttcaaatccc cctctctccg ccactttatc aatgacttat
     1081 ctcccgactt cccgccttgc ttttcctaaa cagaacaatc gtagaatatt cttgaagggt
     1141 tagatcgtca ctgttttctg ttcgatactg tgacattcag cacttgattc gctatggatc
     1201 tgacaggaag ctttcgagcg aaaatgtgca gttattcagt cgttttctta tcgctcacca
     1261 ttattctttt agatattcat cctatgaaac tgccgcaaag ttggtggtgg gaactgaagt
     1321 tgcgtagaga agggttcaat aattgagtca aaaaaggaac gcggccgtca gattaaagcc
     1381 gcgggacaaa gtccataatt tccaagagtt gttgaatttc ccagcttttt tgaaagcctt
     1441 tgtatcatac taacaagtaa aaaaggttta aataccaaaa ttaacctgta tttttgataa
     1501 gtcttaccat tgtacgattt acacagtttc tgctcctcct tgttgatagt ggaaagggtg
     1561 cattgtgcac ttgcgtaaac atttatgacg cgttgatgtc catggttcac atttccttgt
     1621 caggggatcg acttatttgc ggcgaggcat cattcgtaac agagtattat ctttctgaat
     1681 gtagtgatga aagagtgctg cgccagcgtg agctgcgatt aaaaagtaac ctatatttgc
     1741 ccaggtttcg tgaatatttt ttatcagtgc tttaatttcg ctgtttgggg taacaaaggg
     1801 agacacattg aaaccaagga aactccacga ttttccactg taagccatca aagcaatccc
     1861 cagcagagga agtgcaagga aggttatata aagcatgatg tgcattagcg aagccgcttt
     1921 catttgccag gcaggtggcg gtggcacaat agaaggatca ctataacggt gttttataat
     1981 caggcgtgaa aacattaaca cccaaacgaa tatacccgca ttgtaatgtg tttctcgcat
     2041 cagcagataa gtactactac ctttaggaaa ccagccacgg agttccatcg cggcataggt
     2101 tattgcaacg aaaagcagcg ttatccaatg taaataaatt tgagtttttg agaatcggtt
     2161 catttttgta gctcctgtaa tttttacagt caagctatcc cacgaacctt aaaaattcct
     2221 tatacagatg tggttttaag aatttgttat ttaaaaaatt gatagtgcac attgtaacca
     2281 gccaatataa gcaatggcgt gagcacaggt atctttctct gttggccgta ttgtgtatgg
     2341 aatccgttat tggttaactc aatgcgggtt agcttattaa tatacagaca cgtcatagtg
     2401 atgagcgtgt ctgtagctag cttgcgttca gtggcgtttg tggatggacc agtggcagtt
     2461 atgatgactt actaaagcgg catcgaggcg ttatcatgag aatactcaat gagacatcat
     2521 ttcctcgacc acttcttcgc tactcaactg atatggataa tacgttggcc agttttccat
     2581 ttcatttaat aaagattgct gtgaatcatt acccataaaa atgtgaaaat gagatgattt
     2641 tcgtggtgca attatatgat cgctaaattg tatatattta ggggctttgc tttcaggatc
     2701 tttacattcg aataagtaac gaacgccttt cttgcctgat ttataggtga gtattttgta
     2761 tccatcgtaa tcatatttac aggatgttgt ttcattattt ctatggaatt caacaatgcc
     2821 gtcctcaatg ccaatcatct cgatatctgt tgcataacct ttgtgataat aatctttaat
     2881 ttcagcaaat gttttagttt tatctgcatc cgctttcttc tgaaagacgg ggtcaagttt
     2941 gccactttgc agtaaaggat aaacggattg ccagactcca tcccagtcac tgagcgttcg
     3001 gttttgtaca ttggcatcat caaaaacacc attagcagct ttttgttcga cctctgttaa
     3061 gggtttgccg tgtgagtgat gaccatgcga aaaggcagga gcgctaacaa taaagacacc
     3121 taaagcaaca gccagtttgt gaagacgaat cgccaaaaca gtttcctcca gagtcattgt
     3181 atatgatgtg taatgttata ttgtaacata tggcaaaatg caacaactct taggagatga
     3241 gagcatagtt ttctcaggtg tacttatcta tcttattgaa ataaaatgaa ttatattgat
     3301 aatttgaggt tgatgacaaa cgaaacataa tctgatgtga tctgcgtagg ccggataggg
     3361 cgctcgcgcc gcatccggca gggataacgc gtactttgtc ggcaatatga agttatacag
     3421 ataatttgtt gtgaacctgc ttgcgtaacc ggttaaaaag ggaacgcaac ttcttatacc
     3481 gtaaggctaa aagcagtaca gccagcccag cgtagatgag cggctgcggt gaaataatct
     3541 tcactgacca cagataatgt attggggcca ggatcgcgac aagatagacg aaattatgca
     3601 actgttgcca gtgcttgccg agttttcgct gcatcgcctg ggttgaagta aacgctaaag
     3661 caagcaaaat aacccagctg ataattccta acgttaaata aggccgggta attaactcct
     3721 tgcctaataa cgccagatta ttcacgccca gctccagcaa tgcgtaactg gttaaatgca
     3781 gtgtcgccca ggcaaagcac cataagccta acaggcggcg agtgcgtatc aataacggct
     3841 gttttgcgta gcgtgccaga ggtgtgatta acaaggtcgc cagcaaaaat ttcagtgcag
     3901 tgcgaccggt aaaatgctga atatctttca ccggatcggc acccagtcca ccgtgattga
     3961 tcgcccagac cagccagaga aacggcaaca atccggcaag atgcaggcta actttcagcc
     4021 atgtcacctg ttttgctgtc agacgcattc agaaattctc ccgcaaatcc aggccacgat
     4081 acagcgatgc cacctggtcg gcgtaaccat taaacagtag cgttggctgg cgctgaacat
     4141 cgaggatgcc gcctgaacca ataaatcgtt cggtagcctg tgaccagcgc gggtgatcaa
     4201 catgcggatt aacgttggcg taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg
     4261 ttggcggacg ctcgcgggtc agcttaatac tgactatcga tttaatccct ttaaagccat
     4321 atttccacgg cacaatcagt cgtaccggcg cgccattttg tggcggtaac gccttgccat
     4381 aaacacccac ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc aatccttcga
     4441 cataaggata tttcagcccg ccgccgataa agcggtcctg ctggcctggc atttgttccg
     4501 gtgcataaat tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg
//
LOCUS       DAEVZA010000240         4473 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEVZA010000240 DAEVZA010000000
VERSION     DAEVZA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.240"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            220..1776
                     /locus_tag="LAF92_004964"
     CDS             220..1776
                     /locus_tag="LAF92_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9793848.1"
                     /translation="MAEKNKKTITGQVLNSIKINKLKCINGLNEIIFKPHALTAILGP
                     NGSGKSTILHAIASIYMPEEGFPGEDHRLMHFFPRSPHAEWNGSDFIVNLTYRKDGVM
                     IENELKNYGKADIRGSRWIQIYARRPLREVYYLGIDKCVPIIESEKKNNIQYETSSVS
                     NDLITNILHYASYILNKPYTSFNQHQQPNGKILIGVESGGLAYSSLSMSAGEQKIFLI
                     LETILKADKNALILIDELDLLLHDEALKKLIEVISSHAKDKNKQIIFTTHREMITTLS
                     DKINIRHVVNIQGRSYSFEETKPDAINRLTGESTTPIEIYVEDDLAVAIINKICSSLK
                     ASKYVKIFKFGAASNAFTLLASTLIRGDNLSGKLYILDGDKYSTENEKKTALDKVFTG
                     TESRTYELKAAAEGKVKQFNLPNGVKPEQYIHYLITNVPLDGLGGEYLEIIEAARDIR
                     VELDAHNYISNILTKLGIDRPSGLTRVMDLASRHPEWHQYVSEVTDWLQPVVSDLMER
                     LPENDTVDIT"
     gene            1968..2585
                     /locus_tag="LAF92_004965"
     CDS             1968..2585
                     /locus_tag="LAF92_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HBI9793849.1"
                     /translation="MGKYQEEKKTPIIMVKKRRTLLPPSLSEKTGVIARSETEKMEES
                     VSEGISSPAVDTHTPDAPACKKKKKRRRFPRQPHWTHEFTHDCVEKVKSLFPHLRAEG
                     GGFLPLKIGITNDFAAFLTEHPETELTLDEWSCAISCITTRQVYLQRTAVAGIPRYGL
                     DGLPAGQVSECDALNARRWLAVREQQKLKMKTMQEQSASTEKTER"
     gene            complement(2878..3915)
                     /locus_tag="LAF92_004966"
     CDS             complement(2878..3915)
                     /locus_tag="LAF92_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI9793850.1"
                     /translation="MNSWIPMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLASLLGAVTPFCSCSTVPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLIWVTFGWKVTLLYAIIAAGVSVLASIVLDLLGFERHIVDHKNSASSGCTTKC
                     DDSEASVKRTVKNEQNVSACCSSISSEQPAVSCCSSENLGNVNLTTNTTSGPIKLAMK
                     DALQQFKDVLPYLLLSVLIGSFIYGFIPSEWIAAHAGADNPLAIPLSAVVGIPLYIRA
                     EAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIIAFLTVILGMA
                     ILMGYLTQFLF"
     gene            4020..4343
                     /locus_tag="LAF92_004967"
     CDS             4020..4343
                     /locus_tag="LAF92_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI9793851.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PSSTLSHHISALVSVGLVTQNRESRTLMCVSQYEILEAIIEFLREECCVNSKTDVAEP
                     AGKNG"
BASE COUNT         1518 a          906 c          863 g         1186 t
ORIGIN      
        1 ggttaacttt cctgcgccac cagtggatag gagatatgat cagtcataac attaagtcta
       61 aaattacaga catctcaaaa agtctgtagc ataaaagagg cctgatgtag aatactgtgc
      121 caaatagaat attaaaagga tttgatatat cccctgttca cagttatatg tttctgaatt
      181 acatggtgaa tccccccaac ataatgctag gatttgaaaa tggcagaaaa aaataaaaaa
      241 accattactg gtcaagtctt gaactccatt aaaattaaca aactaaaatg tataaatgga
      301 ttaaacgaga ttatttttaa acctcatgcg ttaaccgcaa ttttaggtcc aaatggaagc
      361 ggaaaatcaa caatactcca tgctatagca agcatataca tgcctgaaga aggtttcccc
      421 ggtgaagacc atcggttaat gcattttttt ccacgcagcc cacatgctga atggaacggc
      481 agtgacttca ttgtaaattt aacttatcgt aaagatgggg taatgattga aaatgaattg
      541 aaaaattatg gaaaagcaga tattcgaggc tcacgatgga ttcagattta tgctcgtcgt
      601 cctttaagag aagtttacta tcttggcatt gataaatgtg ttcctataat agaatcagaa
      661 aaaaagaata atattcagta tgaaaccagc agtgtcagta atgatttaat aacaaacatt
      721 cttcattacg cctcctatat acttaataaa ccatacacaa gtttcaatca gcatcaacaa
      781 cccaatggaa aaatattgat tggagttgag tcagggggac tggcctactc atcattgagt
      841 atgagcgcag gagagcaaaa gatattttta attctggaaa caatattgaa agcggacaag
      901 aacgccttaa tattaattga tgaattagat ttattgttgc atgacgaagc attgaaaaag
      961 ttaatagaag tcatatcatc tcatgccaaa gataaaaaca aacaaatcat ctttactacc
     1021 catagagaaa tgataaccac tttatcagac aagataaata taagacacgt cgtaaacatt
     1081 caaggccgta gttattcatt tgaagaaaca aaacctgatg ctataaacag attaacaggt
     1141 gaatcaacga cgcctattga aatatatgta gaagatgact tagctgttgc cataataaat
     1201 aaaatatgtt cttcacttaa ggccagcaaa tatgtaaaaa tatttaaatt tggcgcagct
     1261 tcaaacgcat tcactctact tgccagtacc cttattcgtg gtgacaatct atctggtaaa
     1321 ctttatattc ttgatggcga taaatattcc acagaaaatg aaaagaaaac tgctcttgat
     1381 aaagttttca ccggcacaga atcacgaact tatgagttaa aagcggcagc ggaaggaaaa
     1441 gtaaaacaat ttaatctccc aaatggggtt aagcctgaac aatacattca ttatcttatt
     1501 acaaatgtcc cactggacgg attgggtggc gaatatttag aaataatcga agcagcaagg
     1561 gatatcagag ttgaacttga tgctcataat tatatctcga atattttaac taaattgggc
     1621 attgatcgtc cgtccgggtt aacacgagtg atggatctgg cctccaggca tcccgaatgg
     1681 catcagtatg tgagtgaagt cactgactgg ttacagcccg tagtttctga tttaatggaa
     1741 cgattacctg agaatgatac agtagatata acttaaaata tccaaccatc ccgaatgagc
     1801 aaatacaaaa actcatttgg gataataatt aatataatta tgctggctca tttcatgcct
     1861 gtcaaaaaaa aacaaatttt gctctgttgt tttcttaacg aagatattat gttaatcgtg
     1921 aaaatatgat atatattatt atataagaac acatcgtaga ggtttttatg gggaaatatc
     1981 aggaagaaaa gaaaacaccc atcattatgg tgaaaaaacg tcgcactctt ttaccccctt
     2041 ccttatctga aaaaacaggt gttatcgcac gttcagagac agaaaagatg gaagaatcag
     2101 tctctgaggg gatcagttcg cctgccgtcg atactcatac ccctgatgct cctgcctgta
     2161 agaaaaaaaa gaaaagacgc cgttttccac gccagcctca ttggactcat gaatttactc
     2221 acgattgcgt ggaaaaagtg aagtctctgt ttccccatct tcgcgcagaa ggcggaggat
     2281 ttcttccact gaaaattggc ataaccaatg attttgccgc attcctgaca gaacaccctg
     2341 agacagaact gacactggat gaatggtcct gtgcaatatc ctgcatcacc acccgacagg
     2401 tatatctgca gcgtactgct gtcgcaggta tccctcgtta tggactggat ggactaccag
     2461 caggtcaggt atcagaatgc gatgcgctga atgccagacg ctggctggca gtaagggaac
     2521 aacagaagct aaagatgaaa accatgcagg aacagtccgc gtcaaccgaa aaaactgaac
     2581 ggtaatttca tcgcgataac agcaggttac aaacacaata atattcattg tgaagtattc
     2641 ctttcagatc agcttatatg ttttttgcag gctgctggta gaacagttgc ttttacttgt
     2701 ggtcagtaag cagagatatt ccgacatggg cattcttcag gctatagcct tacaggatca
     2761 atcaccacct ccctaggagg tggtttaggg ataacaataa aagggctaca ccgttagaac
     2821 cgcgccgtaa gactgaaggg attttcatat tgacgcaaaa atcccttctc ataaggatta
     2881 aaacaaaaat tgcgttaaat atcccataag gattgccata cctaagataa ccgtcagaaa
     2941 tgcgattatc atgggcattc tgaacattga tttaagcaat attacttctg tcagactcgc
     3001 tccagcacta ccgattatca aggccattaa tgcccccagg cccattcctt tagtcatcag
     3061 cactgatgca agtggtatca ctgcctctgc gcggatatac aacggaatac caacaactgc
     3121 actcagcggt atggctaagg gattatctgc tccggcatga gctgcaatcc actctgaagg
     3181 gataaagcca taaataaatg aacctattaa tacacttaac aaaagataag gcagaacatc
     3241 cttaaactgc tgtaaggcat ctttcatagc taactttatt gggccagagg tggtattcgt
     3301 tgtgagattc acattgccca gattctcgct gctgcaacag ctaacagctg gttgttcaga
     3361 tgatattgat gaacaacacg ctgatacgtt ctgctcgttc tttaccgtcc tttttaccga
     3421 agcttcggaa tcatcgcatt tcgtggtgca accacttgat gctgagtttt tgtgatcaac
     3481 aatatgacgt tcaaatccta gtaaatccaa tacgatactg gcaaggactg agacaccggc
     3541 agcgatgatc gcgtacaaca aagttacttt ccagccaaag gtaacccata ttaaccccac
     3601 gataatggga ttaagcaacg gtgaaacaaa caaaaaagtg agtgtcggac caaaccccgc
     3661 tttcgcagac aacaatcctc gcaacattgg aactgttgag caactacaga aaggggtaac
     3721 ggctcccagc aacgaggcca gcagataccc cttacctttt ctcgccccca tcatctgctg
     3781 aattttatga tccgggactt tttgccttat caggctcaca ccggcactaa tgacaataaa
     3841 cagcaatgaa agctcaacag ccagaaaaac aaacatctct gcggcatctt gcaacatagg
     3901 aatccaacta ttcatactaa acctcttgac agaatctatt attctagaat aatcgaaata
     3961 taattatagt caagatattt ctggtattat cgaaatatca tcaggcagag ggttgtcaca
     4021 tgcaactgga agaagtagct aaagctctaa aagaactggg tcacccgacc cgtttgttca
     4081 tattcaaaca tcttgtaaaa gctggagaac aaggtttacc tgtaggtgaa ttgcaaaagc
     4141 agcttggcat tccttcatca acgttaagtc atcatatttc agccctggta tcagtaggac
     4201 tcgtcacaca aaaccgggag agtcgaacgc tcatgtgcgt ctctcaatat gaaatactgg
     4261 aagctatcat cgaattctta cgtgaagagt gctgtgtaaa cagtaaaaca gatgttgcgg
     4321 aacccgctgg caaaaatggg taagtaaccg gaaaactcat aactgtcatg attgacgaat
     4381 gctgtatcta cccgtgcgaa gcacacctaa ttaagccaag aatatataga gaaaatcata
     4441 aaaattaata ggttaaataa tatagttcct gat
//
LOCUS       DAEVZA010000241         4457 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEVZA010000241 DAEVZA010000000
VERSION     DAEVZA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.241"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..340)
                     /locus_tag="LAF92_004968"
     CDS             complement(<1..340)
                     /locus_tag="LAF92_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU/TssL family secretion system protein"
                     /protein_id="HBI9793852.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     LVRERLKDAGFSRESVEHITYAQCALLDETVLSRSGMDDGQAIWMKDPLQSHFFNTLQ
                     AGELLYERMKQ"
     gene            complement(337..1674)
                     /gene="tssK"
                     /locus_tag="LAF92_004969"
     CDS             complement(337..1674)
                     /gene="tssK"
                     /locus_tag="LAF92_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HBI9793853.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALTSDSAVVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALELGSLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVSLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRA"
     gene            complement(1692..3230)
                     /gene="tssC"
                     /locus_tag="LAF92_004970"
     CDS             complement(1692..3230)
                     /gene="tssC"
                     /locus_tag="LAF92_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HBI9793854.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGENVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            complement(3289..3789)
                     /gene="tssB"
                     /locus_tag="LAF92_004971"
     CDS             complement(3289..3789)
                     /gene="tssB"
                     /locus_tag="LAF92_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBI9793855.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLSGSGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
BASE COUNT         1089 a         1110 c         1136 g         1122 t
ORIGIN      
        1 cctgcttcat ccgctcgtac agcagctcgc ctgcctgcag ggtgttgaag aaatgcgact
       61 ggagtggatc cttcatccag atggcctgac cgtcgtccat gccgctgcga ctcaggactg
      121 tttcgtccag cagggcgcac tgcgcgtagg taatgtgctc cacgctctcc cggctaaacc
      181 cggcatcttt caggcgttcg cggaccagtt ccacctgccg ctggcagtga cgatacagct
      241 ccataccgtg acgcacggtg gttccctgac gcagttccac caccgtcaga aacgtgtcgc
      301 gcagcagcgc gtcgatattg atatccgtct cttttttcat gcgcgcagca ccgcaaacag
      361 ttcaggtttc aggtcgccca tggttcctgg cgcataaatc atgcagcacc cggactccag
      421 catcgattta gccgcgtcac tgtgcatatc cagcgcaaaa tactggttct ccagtcgcag
      481 cggcagcgcc gccggaactg aggtcagtgg caccagtgaa acaccgttca gcgccacgtt
      541 gataagcagc gtgacgtcat caggcgcacc gattttgcag accagcggta gctggtgcag
      601 tacctgatgc gcaggcagag aggaacgaac ggacagataa aaatcggcct cttcccgcag
      661 gcgcggatcg tgcaggtctg ctttccagcg gttgcccggc agggatccca gctccagtgc
      721 gatcacccgt gacggcaggc tggcttccag cagttcgctg agcagggaga acaggggcgg
      781 aaagacggtg gacggcgatt cgtggacata gggcgggacg gcgctgacgt tatgttcaag
      841 ggaaaatgtc agcagcgccc cggcaagcct cgccagctcc cgccagacca gttcggggtg
      901 aaccgccggg tagcgcagga aatcactcag caccggctcg gcggagttca gcgcgttaag
      961 caaccagaac agggagacat cggcgacggc gaagtccgcc atacgctggt tgctttcccg
     1021 acgcatcgcc atcaggcgct gacatttggc ccgtagctgc aacagcagcg tgtccagacg
     1081 ctgtaccagt ccgtcatggg cgttaaacgc cagtaacggc ggaatataac tctcatcctg
     1141 tgtccagttg ccctgaacat cccgcaccag acgcgccagt gggcaggtga tgtaatcctg
     1201 attgttatca tgggcaaagc gcagggacag cgcatgacgc tcaacggcca tatcttcact
     1261 gtcactgccg tagatatcct gcaccttttg ccattcctga cgatagcgca gcggtctttc
     1321 agtctgctca ccctgaccga gattaccgcc gttaccatgg gcaagcggca gcgccagcag
     1381 aacaaccgcg ctgtcgctcg tcagcgcgcg cagatcgcag gcgagaggca gaacatcgct
     1441 cacatctgaa tcgatcaatg tgccatcagc aaaacgcagg cgtactgcct gcgttttcag
     1501 ctttccggac gtcagagcat cgcggtcaaa gagcacattc gcgatccccc acggatgacg
     1561 aatacaaagt tgcgcaatgc tgtcattgct gaacgcttcc cagcgggcct gctgctgaaa
     1621 ctgctgagag gacaggaatg tcccctcagc ccacagcggt cgatgaattt tcatatcaca
     1681 ccacttcccg gtcaggcttt cgctttcggc atctgggaaa ccagtgacag gttgatatcc
     1741 atcccttcaa tctggaaatg cggcacggca aagagtctga cgcggaagaa gcccggattg
     1801 tcctcaatat cctccacgat aaccttgccg tcgcgcagcg ggtgagacgc ctgcagttca
     1861 tcacccggat cggtcatctc cgtcaccagt gtgcggatcc aggtgttcag ttccagttcc
     1921 agtacgcggc ggtctttggt ggtaccgata ttctcgcgct ggatgatttt caggtaatgc
     1981 gcaatgcggg acagcaggaa gatatagggt aaacgggcgt taatacggct gttggcggtg
     2041 gcgtcagcgg tatcatacaa cgccggtttc tgggcagagt ttgccgagaa gaagcaggcg
     2101 taatcgcgat tcttataata agagagcgga ataaagccaa ggttggcaaa ttcaaattcg
     2161 cgggtttccg ggatcatcac ttcggacgga attttgacct gattgccggt gccgagatcg
     2221 taaagatgga tgggcagatc ggccactgcg ccacctgcct gtggaccacg gatctgcacg
     2281 caccagccat tattcacaaa gctcttcacc atattggcgg caaaggcgaa cgaggcgttt
     2341 gtccagagat acttttcgtg atccgggcct ttgacttctt ccacatagtt aaagctgcgt
     2401 accggcaccg tgtccggccc atagggcaga cggcccagca ctcgcggcat cactaatccc
     2461 acatagcggc tgtcatcggt atcacggaac gatttccatt taatatattc tgcgcggtca
     2521 aagtagttgc cgatatcttt aatcgcggcg acttcttcca tcgaattttt aaggaagaat
     2581 ttcggcccga cagaaccgat aaaaggcata tgggaagcgg cagacactct ggaaatattg
     2641 cgcagcaggg caatatcctg cgggctgtta tcaaattcat agctggaaat taatgaggca
     2701 acaggttcgc cacccggcgt atcgtactcc tgaatgtatg tatgacgata taaaccgctc
     2761 tgaatgattt ccggggagtc gtcgaaatcc tgacgcagat gctctttgct gatatccagc
     2821 aattcaattc tgacattttt gcgaaaatca gtgcgctgta cgacgaacca tgtgccgcgc
     2881 cacagcgact cgactttctg gaattcagga tggtgcatga ccgcatcaag ctgtttactg
     2941 attttctgat ccaaatcgtc gatatgaaaa tccagcaggc tcttgtcgag cttgaccacg
     3001 ttctcgcctg atttcgccag acaggaaagt agaacgtgaa tcgccgctgt cacgcgctca
     3061 tcgggtgagg catccgccag tgtttcgctg ttttgccata aatctatttc ctgaatggaa
     3121 gacaccgggg ttaaattaat tttatcaaac agggactgat aaacgccgcc ggactcagtg
     3181 gtggtcagtg ttgcagtttc agaggtggca tgttcttgtt gtacagacat cagcatttcc
     3241 ttttcagtga tcctttcttc tccggtttaa cctgaatatc acttcgggtt aaacgttctc
     3301 ctgcggcgca attttcgcca gttcatcacg cagttctgaa ctgagcgtcg ggtctttcag
     3361 gatattttcc agttcacgtc gaaacgttgc gttatctaac agattggatt tcaggtcacg
     3421 taataaattg cgcatagcga gtaatacacg aagctgtggg atattttttg ctacctgttc
     3481 gggttcaaaa tctttcaggc ttttaaattc aagcgcgata ttttgctcat taccgcttcc
     3541 gcttagtgtg tcttctactg ttaaattaac cgcaggcgaa aactcagcca tgacactgtt
     3601 gaagttgttt ttattgatat caatcttgtc ccgttcggac agcggacgtt gctcttttcc
     3661 gttactgtaa tcgccaacag caagaagctt gagggggagt tcaactttct ttttagcatt
     3721 gcctgtatgc aggtcaagct tgatatttac acgagcggcg ggaacttcat tctggaaact
     3781 gtcggccata tccttttcct catcatccac tttttctgat ggttattgac ataattatat
     3841 tcacgggatt atcgggtgat ttaaataaaa agcaatgatt aggaaaatat aaaaatgaat
     3901 aatgtatttt tctggggaat agatacagtt gtatcatttt agatattatt tagcctcttg
     3961 attatttttc gggagagggc agccttatct ggtgagaaaa tttggcatga aacttaaaat
     4021 aataacaaga taaacttcac aaatttgcgt ttttaagaaa ttgggtagtg tgggagtcta
     4081 ttaaagcatt cattaaaatt attgcccggg cttccacaaa aaattattaa tttttctact
     4141 aattaaaaat tgtgtgcttt ttgtacaata attatataag tgtagcctcc agttgtgata
     4201 ttgaaatgtt aacataatca aaacaaattg atttatagaa tatgtaagtg aagttcattg
     4261 gtggggtata tatttaatat ataaataaga tggatggctt tgattggggg gatcaacggt
     4321 ttactcattg tttcaggctt cactgaaacg gataaggaag cgtaaaaacg aaaaaaccgc
     4381 aagctaaagg tagcgttgcg gtttctgata ttctaataaa ttggttgcgg gggccggatt
     4441 tgaaccgacg accttcg
//
LOCUS       DAEVZA010000242         4406 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEVZA010000242 DAEVZA010000000
VERSION     DAEVZA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.242"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="LAF92_004972"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="LAF92_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI9793856.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="LAF92_004973"
     tRNA            complement(324..399)
                     /locus_tag="LAF92_004973"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="LAF92_004974"
     tRNA            complement(406..480)
                     /locus_tag="LAF92_004974"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="LAF92_004975"
     tRNA            complement(597..681)
                     /locus_tag="LAF92_004975"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="LAF92_004976"
     tRNA            complement(690..765)
                     /locus_tag="LAF92_004976"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="LAF92_004977"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="LAF92_004977"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBI9793857.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="LAF92_004978"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="LAF92_004978"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBI9793858.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="LAF92_004979"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="LAF92_004979"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBI9793859.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHYVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4233..4348)
                     /gene="rrf"
                     /locus_tag="LAF92_004980"
     rRNA            complement(4233..4348)
                     /gene="rrf"
                     /locus_tag="LAF92_004980"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1129 a         1100 c          989 g         1188 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt tcaggggagc taatgctccc
     2101 ctatcttatt tttccgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatccc caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgc tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtatgagca tggccgccaa cgtattacga tcgagattga ttcccgcttc ctgcagcgtg
     2401 atccaccctt gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggca
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtta gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttaa cacgaacttt atcagcaccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataacgggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc gggacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcaccag agtgaaattc gccattcgct aacagcgcga ttaatttcag cggaacggta
     3061 ttatccttca tgaaatcgtc tccactgcgc taacttcacc tgatgcacca ataaagcgaa
     3121 cttcgggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca taatgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gaggactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatctgcct ggggataatt tggtgctgtt ggaaattgtg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcacct
     3661 gcttgcctgt cgccagttca acacagtcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtaccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tttgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 cggggggatg gcttgtagat atgatgacag gaagagtttg tagaaacaca aaaaggccca
     4201 gtcttccgac tgagccttct gcttaatttg atgcctggca gttccctact ctcgcatggg
     4261 gagaccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag
     4321 gtgggaccac cgcgctacgg ccgccaggca aattctgttt tatcagaccg cttctgcgtt
     4381 ctgatttaat ctgtatcagg ctgaaa
//
LOCUS       DAEVZA010000243         4392 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEVZA010000243 DAEVZA010000000
VERSION     DAEVZA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.243"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..314)
                     /gene="traA"
                     /locus_tag="LAF92_004981"
     CDS             complement(<1..314)
                     /gene="traA"
                     /locus_tag="LAF92_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBI9793860.1"
                     /translation="MDAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAASSSGQDLMASGNNTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFL
                     AG"
     gene            complement(348..575)
                     /gene="traY"
                     /locus_tag="LAF92_004982"
     CDS             complement(348..575)
                     /gene="traY"
                     /locus_tag="LAF92_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HBI9793861.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVTEESESTFKEL"
     gene            complement(669..1355)
                     /locus_tag="LAF92_004983"
     CDS             complement(669..1355)
                     /locus_tag="LAF92_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBI9793862.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALSQSGSNTNTSDKLTIWNDLSPGTLLVF
                     SFYMLGVGHATIARELGITDRASEDRIKPVKRKIKEFFEHFDLFRVSCIYKGKIDLLL
                     NIIREFYSVK"
     gene            complement(1546..1929)
                     /locus_tag="LAF92_004984"
     CDS             complement(1546..1929)
                     /locus_tag="LAF92_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBI9793863.1"
                     /translation="MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQTEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ASMVDDIREKVSVEMDRFFPKNDDE"
     gene            2206..2853
                     /locus_tag="LAF92_004985"
     CDS             2206..2853
                     /locus_tag="LAF92_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBI9793864.1"
                     /translation="MIQKANQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMLAICLMFINGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAV
                     GAYNAGFRKTERQNQRRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(3150..3971)
                     /locus_tag="LAF92_004986"
     CDS             complement(3150..3971)
                     /locus_tag="LAF92_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBI9793865.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(4082..4378)
                     /locus_tag="LAF92_004987"
     CDS             complement(4082..4378)
                     /locus_tag="LAF92_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793866.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
BASE COUNT         1309 a          992 c          845 g         1246 t
ORIGIN      
        1 aaaccggcca ggaacttgac gtttttggtc atcatgtaca tgaccgcacc gaccagaact
       61 tcagccagaa caacccattt aacaatactg gagtccttac cgaaggtcgc cttaaccgtg
      121 ttgttaccac ttgccatcag gtcctgacca ctgctgctag cggccatcgc cagttgcggg
      181 aagaacatca tcagaacagc agccgggatc acagcgcgag ccaggcgaag catattcaga
      241 cgagtgaatt tggaaaaaaa cgacttcttt ttgacgggcg cagaagcacc ctgaacactt
      301 aaaacggcgt ccatatgatt cctctgttta atattgatag ataaaaacta aagttcttta
      361 aatgttgact cgctctcttc ggtcacctcc ctgaatattt cttcgttata gaaatcagga
      421 aatcgtttca gatgatctct gagacggatc tggacttcta tcgttttact tcttccacta
      481 cgatctttgg ccttaataag tcggttattt gtatcttcat cgagatacac cgagactgtt
      541 ctgcttatac cgcctctcgc gttacgcctc ctcactcaat tcacctctgt ttcccgacta
      601 ataaaattat gattgcacag aaaccgacga aaaaaaacac gccaaaattg gcgtgcctta
      661 tttaacattt acttaacgct ataaaattca cgaattatat ttaatagcaa atctattttc
      721 cctttataga tacatgacac tctgaataaa tcaaagtgtt caaaaaattc ttttattttc
      781 cgtttaactg gtttaattcg atcctcagat gctctgtctg taatacccaa ttctctggca
      841 attgttgcat gaccaacacc cagcatataa aaagagaaaa caagcaatgt cccgggggaa
      901 agatcattcc atatcgttaa tttatcagaa gtatttgtat tacttccact ctgacttaac
      961 gcaaaaaatg gataatcagg aaacggatta atttgccata aaacaacaga ttcgtcatta
     1021 taaaaagaaa cattctccat ccttatctga tagatttcgc catgaaaatt aaaacgacga
     1081 caaaatgcag agccatgtcc aagagcctgg cattccaatt caagtgatga aagaaaaatt
     1141 tcaacctgca gacgtatata actctctccg gataagggtt tatcttctct ggaaaagagt
     1201 tcaataaaag ccccattggc ataaaggatt ttccggtttt tgttacggac acaaaccgga
     1261 acgggagaat ttctgtagat attctgaacg ttaaggatgt acttatttac agattgactg
     1321 ttaagtggcc tttctctacg atccagcgca cacataggaa cctccgttaa ggattctatg
     1381 gacagtcgat gcagggagtt cagacctccc tgcatcggcg attttcagtt tcaattttta
     1441 acgtggcatt aaccacgtaa ttaatatact aattatctga cgtattgtca tctttttgaa
     1501 tcataaaaat tcttatattt gaaccaactc aaacttcatt ttcatttatt catcatcatt
     1561 ttttggaaaa aaccggtcca tctcaacaga cactttctct ctgatatcgt caaccatact
     1621 ggcatattca aacttcgggt ttccggagac atgaggactg agagactcaa tacctaaaat
     1681 ctttgccacc gttgactgtg tttttacaac acattcaaga agaagtttat taaactctgt
     1741 ctggttaaac gcagactctt tacgctccat ctgtgcatca tatacgcgaa gccccaattc
     1801 aagaagcatt gaagcagttc ctgaaaggct tatatcttta tctcttgctc cctcctgacg
     1861 acgcttttca acaatcatgt taattttttc atgaacctca ttactgatat acagatttac
     1921 tctggccata agacagtacc ttttatattc tgaaacaagt gttttatttc atttactcat
     1981 agtttggtct gaaaaaagaa gagcacccct agcagcgccc ctaggggcat actataaaaa
     2041 aacgcacggc gctgctaggg gcggccctaa tatatccaaa tgtttttcat aaaatttgtc
     2101 agtactgacc ctaacaaggg tcgccatagg gtcgccatag ggtcgtcaac gcccctattt
     2161 aataatgtaa aataaattaa aatacattat ttaaaacata agctaatgat tcaaaaagca
     2221 aatcagcaaa aacttgtttt tgcgtggggt gtggtgcttt tggtggtgag aaccaccaac
     2281 ctgttgagcc tttttgtgga gtgggttaaa ttatttacgg ataaagtcac cagaggtgga
     2341 aaaatgaaaa aatggatgtt agcaatctgc ctgatgttta taaatgggat ctgcgaagcc
     2401 gccgattgct ttgatcttgc aggtcgggat tacaaaatag acccggattt actgagagcg
     2461 atatcatgga aagaatcccg ttaccgggtt aatgccatcg gtattaatcc ggtaacggga
     2521 tatggcagcg gactgatgca ggtagattcc cagcatttta acgaactggc ccgctatgga
     2581 attaagccgg aacatctgac aacagatccc tgtatgaaca tttataccgg tgcttattat
     2641 ctggcaatag cctttaaaaa atggggcgtc tcctgggagg ccgttggtgc atacaatgcc
     2701 ggattcagga agaccgaacg ccagaaccag agacgtcttg cctacgcatc agaggtttac
     2761 cggatttata cctggataaa gagcagtaaa ggcatccggg ttccgaccac gaagaaatca
     2821 ctttcccaaa ttaacagtgt gcagaaaaat taatattgta tacattgtca aataccggtt
     2881 atgccgcgac agcggcatgg aaggccacac acaaaaatat catatgagtg ataatgctga
     2941 ctggccgaag ccccgcaaag cgggatccca tcttatatat atccggtaac tgcgcagcct
     3001 cctgccagac catttttcgc gggcacaata tttacagact accgaaaagc cagttgatga
     3061 aattgaggca tatcagacga cacagcaatg ccgcaggatg ataatataag taaagccccg
     3121 aaaaattttt cggggcttta cttatattat catgacttcc actccctgaa ctgttcggca
     3181 atcatccata atgctttgtt gattcggata tcaccgtcaa tgcctgtaat ggctcttgtt
     3241 ctggtatttt taccggaggc acttcgcccc gataatccac cttttatcat attctcctga
     3301 acccgctgcc aggttgtcca gagatcgttc tgtttatcct cccaacggcg gggagtaatt
     3361 atttgttctg gcgtcactgg cgttttattt tcatcttcgt acctggccat caatgcagct
     3421 ctgccaaata aatgttgttc gtcactgtta agatgtatct ctttcattgt ctccatattt
     3481 tcagtgactt tatcaaagac accgagcact tcatacgctc cctcaataac ctgcccgaca
     3541 atatcacctt tatgtggaac gcggatttcg ccaaaattat tcccacacac caggccattt
     3601 gtgcagacaa aacgaaaaat tcctgggatc atctgataac tggatgaacc atcatgtgaa
     3661 ttaagcagga taatttcagg aacttctttc ccgttaatat gcccttccct gcgaagacgt
     3721 aacatatgtt tactgtattc gcggcgtccc aaatcacgaa cccgactctg acaggcaaag
     3781 aatggctgga aaccttcatc acgtaactta ttgatgatat tgatggttgg gatatatgta
     3841 taacgctcac tccgggattc atgtttatca ccagaaaaga cagatggaac aaactgcatt
     3901 aattcgtcat ccgttaacgg gcgctcacgg cgaatagaat tatatcgacc aaaacgggaa
     3961 gctaatcgca taacagtaac tcctttatga aggcataaaa ttaataaaag ttttttcgct
     4021 taatatcatc ccccctcggt tatcgttaac aaaaagacaa caattccgag gggaaggacc
     4081 atcaaatcag gtcagttcgg gactgaccaa cctgccgagt gaaaagcagt aacagcgcga
     4141 tcaactcatc agcgtttttt ctgacaagag gagtatgatt ctccaggcta tccagtaagt
     4201 catgcagctc attattgacc tggtggagca ccttaaagag tgcaccagaa tcccggcagc
     4261 tctgatatgc aagatttgcc tgaacagcac gattctgcat gatcccgatt tgtgcgattg
     4321 cgtctttctg ctgatcgcct gttggctgat aagtggtgtt aaaagaaaat gcgctcataa
     4381 ttcctcctgt at
//
LOCUS       DAEVZA010000244         4376 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEVZA010000244 DAEVZA010000000
VERSION     DAEVZA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.244"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..3005
                     /locus_tag="LAF92_004988"
     CDS             <1..3005
                     /locus_tag="LAF92_004988"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBI9793867.1"
                     /translation="QLLAVTSGGTIPDRGMYSVLLPEGEEQAGSRRVGELDEEMVYES
                     RVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEGNGRPAELGEMIGDFLH
                     LLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGSRHLVLERCRDEIGDWR
                     IILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVARIPDTDGKLPDAAIFL
                     FEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQRLRASQLLEI
                     AQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTPSPFATSLLF
                     GYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQVEEELQRLA
                     PGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKRIFPAMISGQ
                     ERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHALVTAEQLAHE
                     FSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREATRPVAATTY
                     ARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPASLWESQILP
                     ARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEYAAESFTPAEADQANRS
                     ALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEALWALVWQGVITSDIWAP
                     LRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPNLAGRWSLLQVEPLNDT
                     ERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSMEDSGRIMRGRFVEGLGG
                     AQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNLLPWPAHPATLVPTRRA
                     GALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTTALRREPRLRFTLTEVN
                     DLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(3056..3403)
                     /gene="grxD"
                     /locus_tag="LAF92_004989"
     CDS             complement(3056..3403)
                     /gene="grxD"
                     /locus_tag="LAF92_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBI9793868.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            3738..>4376
                     /gene="mepH"
                     /locus_tag="LAF92_004990"
     CDS             3738..>4376
                     /gene="mepH"
                     /locus_tag="LAF92_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBI9793869.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVF"
BASE COUNT         1014 a         1146 c         1190 g         1026 t
ORIGIN      
        1 ctcaattgtt ggcggttacc agcggcggca ccattccgga tcgtggcatg tatagcgtgt
       61 tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg gatgaggaga
      121 tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca tggcggatcc
      181 agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct gcccggctcc
      241 ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg atcggcgatt
      301 ttcttcattt actggcggat ggcgcgttct tttccgggac tattcccccg tggctggcag
      361 aagaaaatac gaacgccaat attcaggggt tgattgacga gcaacgcaac gcgacgggca
      421 tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt ggcgactggc
      481 gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg ctggcgattg
      541 ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt gatgacggca
      601 ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt tttttgtttg
      661 aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca cttttcgccg
      721 cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact ccgggccatc
      781 gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc gctcagggat
      841 atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat gtttatgatc
      901 ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa atatccgatg
      961 taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat gtcgcggaat
     1021 ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg tcgctggaca
     1081 gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc gacccgcagg
     1141 tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga gcgaaaggtg
     1201 aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa gacctggcgc
     1261 aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt cttgcagtaa
     1321 aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg gatgatgccg
     1381 ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag atttatttac
     1441 atagagtcag ttacccgctt cgcgatctct ttctgcgcta tctccgggct catgctctgg
     1501 tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc gaagagcagc
     1561 ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc tgggtgagcg
     1621 atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa gcgacgcgtc
     1681 ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta ttacccgcca
     1741 ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag ggcgtagatg
     1801 gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca ctctgggaaa
     1861 gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat gaattactgg
     1921 caaccggtgc ggttatctgg tcggggcaaa aaaaactggg tgaagatgac ggcctggtgg
     1981 cactgcatct acaggaatat gctgcagaat cgttcactcc cgccgaagcg gatcaggcga
     2041 atcgttcggc gctgcaacaa gcgatagtcg ctgttctggc tgacggagga gcctggtttg
     2101 cacaacaaat cagccaacgg atacgcgaca aaatcggcga atcggttgat ctctctgccc
     2161 tgcaagaggc gttatgggcg ctggtctggc aaggcgtcat caccagcgac atttgggcac
     2221 cgttacgcgc cctcacccgc agcagttcca acgcacgcac ctcaactcgc cgcagtcacc
     2281 gggctcgtcg tggacgtcct gtctatgcgc aacccgtctc gccgcgggta tcttacaaca
     2341 caccaaatct ggctggacgc tggtcgttat tgcaggtgga gccactaaac gataccgaaa
     2401 ggatgctggc gctggcggaa aatatgctcg accgctacgg catcatcagt cgtcaggcgg
     2461 tgatagccga aaatatccct ggcgggtttc catcgatgca aacgctttgt cgaagtatgg
     2521 aagactccgg gcgaattatg cgaggtcgtt ttgtagaagg tctgggtggc gcgcaattcg
     2581 ctgaacgtct gactattgac cgattgcgcg atctggcgac acaagccacg caaacgcgcc
     2641 actatacacc agtggcgctc tctgccaacg atccggctaa tgtgtgggga aatcttctgc
     2701 cctggcctgc acatccggca acgctggttc caacgcgtcg ggcgggtgcg ctggtggtcg
     2761 tttctggcgg caaattgtta ctctatctgg cgcaaggtgg caaaaaaatg ctggtctggc
     2821 aggaaaaaga ggaattactc gccccagagg ttttccacgc gctgactacc gcactgcgtc
     2881 gcgaaccacg gctgcgcttt acgctaacag aagtgaatga tctaccggtc cggcaaacgc
     2941 cgatgtttac gctgctgcgt gaggcgggat tttcaagttc gccacaaggg ctggattggg
     3001 gatagagaaa ggactgacgg atgcccgttc gcatccgtca gtattgcagg acggattatt
     3061 ccgcgtccgg ctcttcagac ttgtatttag cggcagtttc tttgatcagc tgctgcagtt
     3121 cgccacgctg atacatttcg atcacgatat cacaaccgcc gaccagctcg ccgtcaaccc
     3181 acagttgcgg gaaggtcggc cagttagcat atttcggcag ttccgcacga atgtccggat
     3241 tctgcagaat atcaacatag gcaaaacgtt cgccacatgc ggcaagcgcc tggactgcct
     3301 gggcagagaa accgcagctc ggcagtttcg gtgaaccttt catgtacagc aggatcgggt
     3361 tttcagcaat ctggcgttgg attttttcga tagtggtgct cattgtcttg cttcctcaaa
     3421 cttctttacg gcagtaatct gacattgtag cgggtcagtg cggcatcgga aaataacatt
     3481 ttcatcacgc ttttgctatt ttatcccttt gctctatttg ttgcatttca aatattcgtt
     3541 tcttctctga tgcgcggcaa actggcgtat ttataaccat ttttagcttt cactgctgca
     3601 tttttttgcg ctcgccaacg aaacgtattt tttaacaata aaagctatta actttctctt
     3661 cttctatgca ttagaatcat caagttttgt aaatcagacg caggcatgat agacctgcct
     3721 taacagaggg acgctcagtg gcgcggataa accgtatttc gatcacgctc tgtgctttac
     3781 tttttaccac cctgccttta acgcctatgg cccatgcttc aaagcaagcc agggagagtt
     3841 ctgctaccac tcatatcacc aaaaaagcag ataaaaagaa aagcacggca accaccaaaa
     3901 agacccagaa aacagcgaaa aaagccgcca gtaaaagtac gaccaaaagc aaaaccgctt
     3961 cttccgttaa aaaatcttcc attaccgctt ctaaaaacgc caaaactcgc agcaaacacg
     4021 ccgtcaataa aacggcctca gccagcttta ccgaaaagtg taccaaacgt aagggctata
     4081 aatcgcattg tgtgaaagtc aaaaatgccg cgtcaggaac tcttgccgac gcgcacaaag
     4141 cgaaggtgca aaaagctacg aaagtggcaa tgaataaact gatgcagcaa attggtaagc
     4201 catatcgttg gggtggcagc tcaccgcgta ccggttttga ttgcagcggc ctggtttatt
     4261 acgcttataa agatttggtg aaaattcgta ttccgcgtac ggcgaatgaa atgtatcacc
     4321 tgcgtgatgc agcgccaatc gaacgtagtg aactgaaaaa cggcgacctg gtcttt
//
LOCUS       DAEVZA010000245         4230 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEVZA010000245 DAEVZA010000000
VERSION     DAEVZA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.245"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(14..265)
                     /gene="trbG"
                     /locus_tag="LAF92_004991"
     CDS             complement(14..265)
                     /gene="trbG"
                     /locus_tag="LAF92_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HBI9793870.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(258..419)
                     /locus_tag="LAF92_004992"
     CDS             complement(258..419)
                     /locus_tag="LAF92_004992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HBI9793871.1"
                     /translation="MTAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSVPVKELIDRII
                     QKYKEPFDE"
     gene            complement(441..578)
                     /locus_tag="LAF92_004993"
     CDS             complement(441..578)
                     /locus_tag="LAF92_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HBI9793872.1"
                     /translation="MNMRNINVITALSVPGKTVSDDFMHAVLSNCATRIVLPAPEKFG
                     S"
     gene            complement(565..1155)
                     /locus_tag="LAF92_004994"
     CDS             complement(565..1155)
                     /locus_tag="LAF92_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HBI9793873.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYIVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(1145..2572)
                     /locus_tag="LAF92_004995"
     CDS             complement(1145..2572)
                     /locus_tag="LAF92_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HBI9793874.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVMYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGGVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLRMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEENQESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(2572..3276)
                     /gene="traK"
                     /locus_tag="LAF92_004996"
     CDS             complement(2572..3276)
                     /gene="traK"
                     /locus_tag="LAF92_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HBI9793875.1"
                     /translation="MLGGLFFSCSVMAGVGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(3287..3853)
                     /gene="traE"
                     /locus_tag="LAF92_004997"
     CDS             complement(3287..3853)
                     /gene="traE"
                     /locus_tag="LAF92_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HBI9793876.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETADASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(3875..4186)
                     /gene="traL"
                     /locus_tag="LAF92_004998"
     CDS             complement(3875..4186)
                     /gene="traL"
                     /locus_tag="LAF92_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBI9793877.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
BASE COUNT          983 a         1205 c         1004 g         1038 t
ORIGIN      
        1 aaagaaatct gtttcattgt ccctcacctt tctcatcgca gttcacaaaa tccttcttca
       61 aatccctgac cgtacaggtt tttacaatgt caggattttc aaaaaccgta ctccgtgatg
      121 atttataaac atatgccaca atgcgtttcc cacccgcatc aggttctggt gcactgactt
      181 cacataacgg aggtgttatt ccggactcat acagcaccgg ataatttatt tttttgacgc
      241 tgccatcaga aaccagctta ttcatcgaag ggctccttat atttctgaat aattctgtcg
      301 attagttctt tcaccggaac agattttccg ccaccggatt gtccggcaac cacaatatgc
      361 cccggagaag aaaataaact catcgacata cccttcttca tcacaccgac agcagtcata
      421 ttaaaattgt ccggcaatga ctaagaaccg aatttctccg gtgcaggaag tacgattctt
      481 gtggcgcagt tactgagaac agcatgcata aaatcatctg acacggtttt acccggcaca
      541 gagagggctg taataacatt aatattacgc atgttcattt ttatctccgt tgtttctgtt
      601 tgttctgtca tcaggaagat aaacagggca tttcctgatt tttcccggca gggaagcgtc
      661 ttccgtataa acgtccttca gatattcatc tgcggttacc acccggatac atttcatttt
      721 tccgggcagc accggatatt tcccggtgat cgctgccagc gagacataca tcagtgccag
      781 tgttgcccat aatgcggtga ccagttgcat cagtgaccgg tcagtctccc tgacataatc
      841 tgcagcatca gccgcgcggt aatggcagat atcctgtatt tctgatctgc ctgaagaatc
      901 agaggaagaa aaccagcatt cactgaccgg gaactgtccg gcaggtgctg tttgtcttac
      961 gaagttaatc tccttaacca gtaatttccc cggcgtggtg ttgtccttcc ataaaacaaa
     1021 cagagccatc agggccattg tggccagtgg cagaatgacg atgtacttca gacaccagaa
     1081 cagtcctcgc agaacccggg cgacaacata ccgggcggca tggcacgcct gacgggacga
     1141 catattattt gccatcgttg ccccctccct ggctgccgga cataaactgc tgcggattca
     1201 ggttgcccag ctgcttcagc atctggtcag tattaaagcc gttaagatga ctttcagccg
     1261 atggcggaac aggaaccgga ctttcctgat tctcttcttc cgctctgctc tgcgtgcgtt
     1321 ccagcgccat ctcttccacg gttttcagct ggaagccgtc ctggaacacc acggtcactt
     1381 cgttgcccgc accaatcggt atcaccgggt gatactgttc ggcacgtttg atgtagtagt
     1441 cactgagcgt ctgtgcggct ttcgatgcgc cgccaccgat acccattctc aggacatcac
     1501 cagccccgta agcggctgtg gcacccagcc cgacagccgg ctgggaggcg cgctccattc
     1561 cctgaccgat accgtcaaca aatcccgcgc cccatgccca gccgaggatt ttgccgttac
     1621 gcatcaccac tccgcccttg ataccgtttt taccccggaa gctgacatgc cccttaatcg
     1681 gcatatcaat agttttgccg tccttcaggc aactgatatt gcgggtgcgt acaatggcac
     1741 gctcactaga cacatccccc caggcttcca gacccacaaa acatcccgtt gcgtcatacg
     1801 tcttgctgtt cggcatttcc accaggccgg tgatacgcag ctgcatcggc accgtggatt
     1861 cattaccggt gacagaggca ttggcatccg ccccttcaat cagcatggct ttcgcaaaac
     1921 ttcctgacgg aatgtacggc agtgatggtc cctgttttcc ctcattacgg gtaaacacct
     1981 tacgctgtat ccggttaggc accggcacag actggtacat cacctgtggt ggtggcgtga
     2041 caccattccc gggataaaac gccgtcggcg gtggaacagc aacgctgccc tgcggcggga
     2101 aagatacggg tgtgtttcct ggctgtggtt cgccttccgg gccgggcggt gaggcaggca
     2161 tctgcggtac aggctcaccg gtcgccgtga cgggattagc acccagggct tttacctgct
     2221 ctgccagggc ggcattgtcc tgtcccaact tttcaatacg acgctggtca tcaccgcgct
     2281 gtttgttcag aacatccagc tcacgacgga tttcctcata ctgcttctgc atctgcgctg
     2341 ccgtcacctg catctctgtg gtggcatgct gacgcacctt gtcatcaaag gtcgtatcca
     2401 ccacaccggt catatccggc acaggctcct gttcagccac ggtttcaccg ttaccggaca
     2461 tgtccacatc agacagatac agtgccccac caatcgcgga ggctgtaccg acaaccacaa
     2521 tccccagcca caggtactgc ttgcgtttca caatggtatt gatactggcc atcagttgcc
     2581 ctccccgtca cggatgacat acacatccat gcgcgcacct gccagtaact ggctggcagg
     2641 ctggctgaac atcacggcac gggttcccgg ctgccagaag tcactttccc ggatattcag
     2701 ggcagacagc gttttatttt ccacggcaaa gcggaccatc ttcaggtgat tccccgtcca
     2761 tacggcatct gccactgaag acagccccgc cggggcctgc agggtttcct ttgtcaccgg
     2821 gacctgatac cagcctgcgg gtaattttcc gccacggacg gcctggctga tggtcacaag
     2881 cagggattcg taaggcgtgg acgtttccca cgcaccggct tcttctccgg ttccgcgcag
     2941 gtcactgacc agctgaatgg tacgccccgc gccttcacgg ggaacggcct gaatggaaag
     3001 attcagacca cgttctgttt ccagaatgaa cgtaaagggc tttttgttga cggtggccac
     3061 caccacaccg ccggaggcgg tctgctcatt attggtcagg gcaccatcca gactgttcac
     3121 ggcgataatc cggtcaccgg gaacggtaaa cagattcggg ctggtattgc tgatggcaat
     3181 actggcctga ccaccgttca ccattggcac cacggtgggg gccagcgtac cgactccggc
     3241 catcacgctg caggaaaaaa acaggccgcc taatattatt gctgtattat tttttctcat
     3301 cgtctgtttc cccgaaatta tccagccagg tcaccccgtt ttcccgcttc agaataagga
     3361 tgtaatgttt gatatcagtg aaaggttttg aatcaccaat ccaggtttta agcacaccac
     3421 gaatttccac acggccatac tgaggccaga cacgaacact ggtctggaaa aaggctgagt
     3481 tcacgttatc gtttttaata cgcttcgctt cttcagccag aataactttc atctggttct
     3541 gtgcgcccgg gcggatatat tgcagaagcg cctgatgtga ggcatcggcg gtttctgatg
     3601 aaacattcag acggagggca ataaatgaca gcgccatctg ctgtaaataa gaggcgtcgg
     3661 cactgttctg tgacacggca aagggggcat taaatgccat tggcgtcacg gcagtgcgtt
     3721 gctcattctg aagacggtag ttattcaccc cctgaatgac gttaacagag aggctgagaa
     3781 caataagcac tgacataaat ataaaggcga tggccattac acgactggta cttaaacggg
     3841 caccgtgttc catataacaa tcctggtatc agttctattt aatccactgc cggaaacacg
     3901 aatcgggaac attatgaaaa ataccgcgca gcagggctgt tggcatatac cagtaaatca
     3961 ggtcacgtaa ccaggaactg ccccgccctt ttttcagttt tttaatcccg aaataaacca
     4021 gaaccgctgc accaataccg aacagatatt tcgatgttgt gataccccag ccaatacaga
     4081 ttgctgcggg gatcagttca tccagtggca ggccaaacca gcggctctgg ttggtcagtg
     4141 tttccgggaa acgatatttc ttaagtttat tctcgtctcc cgacatcgtt ttatttcctg
     4201 tcagaggcca acgacggcca tacccacagc
//
LOCUS       DAEVZA010000246         4086 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEVZA010000246 DAEVZA010000000
VERSION     DAEVZA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.246"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..126)
                     /locus_tag="LAF92_004999"
     CDS             complement(<1..126)
                     /locus_tag="LAF92_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="cytochrome b556(FDO) component; heme containing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBI9793878.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQW"
     gene            complement(119..1003)
                     /gene="fdnH"
                     /locus_tag="LAF92_005000"
     CDS             complement(119..1003)
                     /gene="fdnH"
                     /locus_tag="LAF92_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBI9793879.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(1016..4063)
                     /gene="fdnG"
                     /locus_tag="LAF92_005001"
     CDS             complement(1016..4063)
                     /gene="fdnG"
                     /locus_tag="LAF92_005001"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3476..3478),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBI9793880.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT          874 a         1179 c         1039 g          994 t
ORIGIN      
        1 ccattgcagc gtcgggaaga agaacgaaat cccggacagc gccaccagga agaagcaaat
       61 caccacggtc cagtgacagg cgcgatcaat aaatttggtg cgcacaatca ttttcgactt
      121 actcatgatg atcctcctcg tcatcgtcca cttccttatt cgggccaata ccgatgtagt
      181 ggaaaatcaa cccggcaaaa gtggcaataa agccagccgc tgccagcggt ttcaacgcgc
      241 ctttccacag gcttaccgag gtgtcgatct tcggatcttt cggcagaccg tgatacagct
      301 ccggctgatc ggcgtgatgc agcacgtaca taacgtgcgt accaccgacc ccttccgggt
      361 tgtagacgcc agcatgttcg taaccacgcg ctttcagttt cgccacgcgc tgttccgcca
      421 gctccagcat ctccttcttg gtgccgaagt ggatagcccc ggtcggacag gttttcacac
      481 aagccggttc ctggccgacg ctgacgcgat cgacgcagag cgtgcattta tatacccggt
      541 tatcctcttt gttgaggcgc ggaatattaa atggacaccc ggcaatgcag taaccacagc
      601 cgatgcagtt ttccgactgg aaatcgacaa tcccgttagc gtactgaatg attgcaccag
      661 cagacgggca cgccttcagg cagcccggat cttcacagtg catacagccg tctttacgga
      721 tcagccactc cagcttgccg ttctgttcgg tttcgctaaa gcgcatcacc gtccaggact
      781 tggcgctcag atcggcgggg ttatcgtaaa ccccgacgca gtgccccact tcatcacgga
      841 tgtcgttcca ctccgaacac gccacctgac aggctttaca gccgatacag gtggaaacgt
      901 cgataagttt cgcgacttct gctttgtaat cacgcacctg agaaggcggc gtgatggagt
      961 tagttgcgga ccttttgata atgtcctgcg tttccatagc catctgttcg cccccttacg
     1021 ccttctcgat gttgactaag aacgctttat attccggcgt ttgcgagttt gcatcaccga
     1081 cattcggcgt cagagtgtta gcgatataac ctttacgcgc gacaccctca aagccccagt
     1141 ggattggaat acccaccgtt tcaacctgct gaccgtttac attcagcggt ttcagacggc
     1201 gcgttaccac cgccaccgcg cggataaagc cacgcttgct tgagacagtg acacgatcgc
     1261 cattattaat gcctttcgcc gccgccagcg tttcgctgat ttccacaaac tgttccggct
     1321 gagcaattgc gttgagcaat gcgtgcttgg tccaggtgtg gaagtgctcg gtcagacgat
     1381 aggtcgtacc cacatacggg aactgctctt ttttacccat ccgcagcgcg tcttgttcat
     1441 acagacgaac aaccgggtta gacaccacgt tcggatgcag cgggttagtg cccagcggcg
     1501 tttcaattgg ctcgtagtgt tccgggaacg gaccttccgc cattttgttg atggcaaaca
     1561 ggcgtcccat cccttccggc tgcatgataa acggcccggt tggcgtaccc ggtgcggcat
     1621 tgccgaagtc cggaatatcg ttacccgtcc acttgctgcc gttccactgg atcagcatcc
     1681 gtttcggatc ccacggttta ccgttgatat ccgccgaagc acggttgtac agcacgcgac
     1741 ggttgagcgg ccacgcccag gcccatccca gcgtattccc cagaccggac gggtcggagt
     1801 tatcgcggtt agccatctgg ttgccctgct ctgtccagct accggtgtag atccagcaag
     1861 aagatgcggt tgtaccgtca tcacgcagat gcgcaaagct actcagcaac tgacctttct
     1921 tcgcaatcag cacgccattt gcgtcataga gatcttccag cgcatagccg ttgttctctt
     1981 ttgccacttc gtcagattgc ggttcgtgcg gctgcttgta gttccagctc atcttcatca
     2041 gcggttctac gcctttacca ccttcggcct ggtacagctc gcgcagatga tggtagatac
     2101 ccgccagaat ttcgccgtcg ttacgcgctt cgcccggcgc gtcctgacct ttccagtgcc
     2161 actgcaacca gcgaccggag ttagcgatag aaccatcttc ttcggcaaag caggtcgaag
     2221 gcagacggaa tacttcagtc tgaatagacg ccggatcgac atcgttcgac tcgccgtggt
     2281 tctgccagaa ggtagaagtt tcagtcacca gcggatcgat aaccaccatg tacttcagct
     2341 tgctcaggca gctcaccact ttgtttttgt ccgggaagga cgcaaccggg ttaaagccct
     2401 ggcagaaata accggtgact ttgccttcat ccatcatgtt gaaatacttg atgacgtcgt
     2461 aggtctggtc ccacttcggc agccagtcat agccccagtt gttctctttc tgcgcggcat
     2521 cgccatagaa agatttcatc aggctaacga agaacttcgg atagttgctc cagtagttca
     2581 cctgatcagc cagcgtcgct ttcggcgtgt tcgcttccag atacgactgc aaatcaacct
     2641 gtttttctga cggcagcgtc agataacctg gcaggctggt agagagcagg cctaagtcag
     2701 tcagaccctg aatgttggag tgaccacgca atgcgttcac gccgccaccg gccataccca
     2761 tgttaccgag cagcaactgg atcatcgcca tagtacggat gttctgcgca cccacggtgt
     2821 gctgcgtcca gcccagcgcg tacaggaagg tggttgtgcg atccggtgcg ctggtggagg
     2881 ccagcacttc acacactttc aggaagtcgg cttttggcgt accacagatg ttttcaacaa
     2941 cgtccggcgt gtagcgggaa acgtgcgctt tcagcaggtt ccacacacag cgcggatgag
     3001 tcagtgtttc atcgcgtttc gcatagccgt tttcatcgaa ctgatagttc caggacgatt
     3061 tatcgtattg ccgtttttca gcgtcgtagc cgctgaacag accgtcttcg aaagcaaaat
     3121 catcacgcac cagcaggctg gcgttggtgt aatgtttaac gtattcggcg ttgattttgt
     3181 tgttttcgat caggtagcgc aaaacgccag acaggaacgt aatgtccgta ccggaacgaa
     3241 taggtgcgta aatatccgcc acagaagcgg tacgcgtaaa acggggatcg acaacgatca
     3301 aggttgcatc gttgttgttt ttcgcttcca tcgcccagcg gaaaccgacg ggatgcgctt
     3361 cagcagcgtt accgcccatt accattacga cgttagcgtt tttgatatcc acccagtggt
     3421 tggtcatcgc accgcgacca aatgttggag caagacttgc taccgttggt ccgtgtcaga
     3481 cgcgcgcctg gttgtctacc gccagcatcc cgagggagcg ggcaaatttc tgtgtcagca
     3541 tcccggtttc gttgctggca ccggaggcac acagcatacc ggtagaaagc caacggttta
     3601 ctgttacgcc ctgctcgttc ttttcaataa agttagcgtc acggtcagct ttcatcagct
     3661 ttgcaatacg ggagaatgct tcttcccagc taatgcgctg ccatttgtca gaacctggcg
     3721 cacgatattc cgggtagcgc agacggtttt cgctgttgac gtaatccagc aaaccggccc
     3781 cttttgggca cagcgcacca cggcttaccg gatgatccgg gtcaccttca atgtgataaa
     3841 tcgcttctct ggcgtttttc gcgccatcac ccaggctata catcaatagc ccgcaaccta
     3901 cggaacagta tgtgcaggtg ttacggatct ctttagcgcg taataatttg tagtttcgcg
     3961 cctgagccag tgcttgcttc ggggcaaagc ccaatgccgc taccgttgtt ccagccatac
     4021 cgcccgcgca gattttaaaa aattgtctgc gactgacgtc cattgctttc ctcttttttc
     4081 aggggt
//
LOCUS       DAEVZA010000247         4051 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEVZA010000247 DAEVZA010000000
VERSION     DAEVZA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.247"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..144
                     /locus_tag="LAF92_005002"
     CDS             <1..144
                     /locus_tag="LAF92_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HBI9793881.1"
                     /translation="VGGGIVAIVPHEMAIAVWSPELDDAGNSLAGIAVLEQLTKQLGR
                     SVY"
     gene            144..503
                     /locus_tag="LAF92_005003"
     CDS             144..503
                     /locus_tag="LAF92_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBI9793882.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            642..2060
                     /locus_tag="LAF92_005004"
     CDS             642..2060
                     /locus_tag="LAF92_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBI9793883.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            2287..3738
                     /gene="uxaB"
                     /locus_tag="LAF92_005005"
     CDS             2287..3738
                     /gene="uxaB"
                     /locus_tag="LAF92_005005"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBI9793884.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT          940 a          937 c         1046 g         1128 t
ORIGIN      
        1 gttggtggcg gtattgtggc gattgttccg catgaaatgg ccatcgctgt ctggagtccg
       61 gaactggatg atgcaggtaa ctcgcttgcg ggtattgccg tccttgaaca attgacgaaa
      121 cagttagggc gttcggttta ttaatgcagt ctctcgatcc actcttcgcg cgtttatccc
      181 gttcaaaatt tcgctctcgc tttcgtctgg gcatgaaaga gcgtcagtat tgcctggaga
      241 aaggcgcgcc agtcatcgaa caacatgcgg cggattttgt cgctaaacgt cttgctccgg
      301 cattaccggc taatgacggt aagcaaaccc ccatgcgcgg gcatccggtg tttatcgctc
      361 agcacgccac cgcgacctgc tgtcgcggtt gtctggctaa gtggcataac attccgcagg
      421 gtgtttcgtt aagcgaagaa caacaacgtt atatcgtggc ggtaatctat cactggctgg
      481 ttgtacagat gaatcagcca tgagaattgc ggaaaacgat aaaatagcat tcccagggta
      541 cctttatttg ccgccgctgt tagcgaacat gctatccttt atgagaattt acttattacg
      601 ttgagataat tgccacccgc aattgtatgg acagataatt aatgcatgta caccccattt
      661 caactttccg tttgttccag gaaggtcatc tgctacgtaa tagcatcgct atttttgcgc
      721 taaccacgct gttctatttt attggtgcag agttacggct ggttcacgaa ctttctcttt
      781 tctggccgct gaatggcgta atggcggggg tgtttgcccg ctatgtctgg cttaatcgac
      841 tgcattacta tgcgatcagt tatgtggcga tgctggttta tgatgccata accaccgaat
      901 ggggactggt ttcactggct atcaatttct ccaatatgat gtttattgtt accgtcgcct
      961 tactggtcgt gcgggataag cgtttgggga aaaataagta tgagccagta agtgccttac
     1021 ggctatttaa ttactgtctg attgccgcat tattatgcgc tattgtcggg gcgattggtt
     1081 cggtcagtat tgatagtctg gatttctggc ctttgcttgc ggactggttc agtgagcaat
     1141 tctcaacggg cgtgttgatc gtgccttgta tgctgacgtt ggctattcct ggagtgctgc
     1201 cgcgctttaa agcagaacag atgatgcccg ccatcgcgct tattgtgtcg gttattgcct
     1261 cggtagtcat tggcggagcg gggagtctgg cgttcccgct gcctgcatta atctggtgtg
     1321 cagtgcgcta tacgccgcag gtaacatgtc tgttgacctt tgtcaccggt gcggtggaaa
     1381 tcgtactggt ggcaaattcg gtgattgata tctcggtcgg ttcgccgttc tccattccgg
     1441 aaatgttctc cgcacgtctc ggtattgcca cgatggcgat atgcccaatt atggtttctt
     1501 ttagcgtggc agcgatcaat tcgctaatga agcaagttgc gctgcgagcc gactttgatt
     1561 ttctgactca ggtttactca cggtccggtc tttatgaggc gctgaaaagt ccatcgctga
     1621 aacagacaca acatctgacg gtcatgctgc ttgatatcga ctatttcaaa agcattaacg
     1681 ataactatgg acatgaatgt ggcgataaag tgttaagcgt gtttgcccgg catattcaga
     1741 agattgtcgg tgataagggg ctggtggcgc gcatgggcgg cgaagaattt gctgttgcag
     1801 tgccgtcggt gaatcctgtc gatggtctgc taatggcgga aaaaatccgt aaaggcgttg
     1861 aactgcaacc attcacctgg caacaaaaaa cgctttatct cacggtaagt attggcgtcg
     1921 gtagtggtcg cgcatcgtac cgaacgctga ccgatgattt taataaattg atggtcgaag
     1981 ccgatacgtg tctgtatcgc tcgaagaaag atgggcgcaa ccgtaccagc actatgcgtt
     2041 acggtgagga agttgtctga catctttatt cattccgcgc gatttgaatg caatgtgttc
     2101 taccacttta tgatccagga aaaggcgtat ttcctcgatt aaaccatgat ccgcgccaca
     2161 cttttctcat aaaactgctc attaatcatc aggagttgat caaaacaggg gttcggcgta
     2221 ttaaagtttg ctaacctgtc gtaccaattc aaacaggttg tatgactaat cagaagggaa
     2281 cccattgtga aaacactaaa tcgtcgcgat tttcccggtg cacagtatcc agaacgtatc
     2341 attcagtttg gtgaaggtaa cttcctgcgc gcctttgttg actggcaaat cgatctcctg
     2401 aatgagcaca ccgatctgaa ttctggtgtg gtcgttgttc gtccgattga aacttcattc
     2461 ccaccgtcac tgagcacgca ggatggtctg tataccacca ttatccgtgg cctgaatgag
     2521 aagggggaag cggtcagcga cgcgcgtctg attcgctcgg ttaatcgtga aatcagcgtc
     2581 tacagtgaat acgatgaatt cctgaaactg gctcacaacc cggaaatgcg ctttgttttc
     2641 tctaatacca ccgaagcggg tatcagctac catgcgggcg ataaattcga tgacgcgcca
     2701 gcggtaagtt atccggcaaa actgacccgt ctgctgttcg aacgttttag ccatttcaac
     2761 ggtgcgctgg ataaaggttg gatcatcatc ccgtgtgagt tgatcgacta caatggcgac
     2821 gcgttgcgtg aactggtgct gcgctatgcg caagagtggg cactgccaga agcatttatt
     2881 caatggctgg atcaggctaa cagcttctgt tctacgctgg tggaccgtat cgttaccggt
     2941 tatccgcgcg atgaagtggc taaactggaa gaagagctgg gttatcacga tggttttctc
     3001 gacaccgctg aacactttta cctgtttgtg attcagggac cgaaatcctt agcgactgaa
     3061 ctgcgtctgg acaaatatcc gctcaacgtg ctgattgttg acgatattaa accgtataaa
     3121 gaacgcaaag tggcaatcct caacggtgcg cacaccgcgc tggtgccggt ggcgttccag
     3181 gcagggctgg ataccgtagg tgaagcgatg aacgatgctg aaatttgcgc attcgttgaa
     3241 aaagccatct acgaagaaat tattccggta ctggatttgc ctcgtgatga actggaatct
     3301 ttcgccagtg cagtcaccgg gcgtttccgt aacccgtaca ttaagcatca gctgctgtct
     3361 atcgcgctca acggtatgac caagttccgc acccgcatcc tgccgcagct gctggcaggg
     3421 cagaaggcaa acggcacact tccggcgcgc cttactttcg cattagcggc attgattgcg
     3481 ttctatcgtg gtgagcgtaa cggagaaaca tatccggtac aggacgatgc acactggctg
     3541 gaacgttatc agcaactctg gagccagcat cgtgaccgtg ttatcggtac ccaggaactg
     3601 gtagcgattg tgttggcaga aaaagaccac tgggagcagg acctgacgca agtgcctggt
     3661 ctggtcgagc aggttgctaa tgacctggat gcgattcttg aaaaagggat gcgcgaagct
     3721 gtacgtccgt tgtgctaata acaccgttac ttctgaaccc ggtttcgacc gggtttctta
     3781 tttcactata ttagttacaa catcctatgt gcctttatca ttaacaggtg ccacatgcag
     3841 cgcctgttaa tgttgtaagc ttgtaggtaa tttgtaagta gcgtcttgag aatgtgatca
     3901 taatcacgtt taatagggcg actttttcac gcctgaagat aaccatgatt gttcgtccac
     3961 aacaacactg gctgcgccgt atttttgtct ggcacggctc agtattatcc aagatatcat
     4021 cgcgcttact actcaatttt ctcttttcta t
//
LOCUS       DAEVZA010000248         4034 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEVZA010000248 DAEVZA010000000
VERSION     DAEVZA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.248"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            35..274
                     /locus_tag="LAF92_005006"
     CDS             35..274
                     /locus_tag="LAF92_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793885.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(417..1208)
                     /gene="kdgR"
                     /locus_tag="LAF92_005007"
     CDS             complement(417..1208)
                     /gene="kdgR"
                     /locus_tag="LAF92_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBI9793886.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            1385..2758
                     /locus_tag="LAF92_005008"
     CDS             1385..2758
                     /locus_tag="LAF92_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9793887.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(2804..3685)
                     /gene="htpX"
                     /locus_tag="LAF92_005009"
     CDS             complement(2804..3685)
                     /gene="htpX"
                     /locus_tag="LAF92_005009"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBI9793888.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT          939 a         1044 c          936 g         1115 t
ORIGIN      
        1 cgattaagcg aacataaaaa gagaagaggt tgtaatgcga ttcatcattc gcacagttat
       61 gctgatagca ctggtatgga taggattatt actcagcgga tatggcgttt tgattggaag
      121 caaagagaat gccgcaggat taggtttgca atgtacctat ctgactgcca gaggcaccag
      181 cacggtacaa tacttgcaca ctaaaagtgg tttcttgggg ataacagatt gcccactgtt
      241 acgtaaaagc aatatcgtgg tcgataatgg ttaagggtaa taaataacca gattctcact
      301 catgagtaaa atattcttat agcaagcgcg tttaatataa cgcaccttca actttatttt
      361 tctcatgcta aataaagaac ctgtctgttt gatattccga caggttctta ctctcatcag
      421 aacggatagt cgtgataacc catttgggca gaaattttgc gcgctgcggt atgcaacatt
      481 gcgacatatt cctgtaaacg ctcttcagag aaacgcaacg tcgggaagga gatgctcaaa
      541 ccggcaatga ccacgccaaa gcgatcaaat accggtaccg caatgcatcg cagcccttct
      601 tcctgctctt cgttatcttc gccatacccc tgctcgcgca cctggtccag aacgggtaat
      661 aacgcttctg tactggtgat ggtccgctcg gtactgcgtt tatactccac gccctcaaga
      721 atttgcttca cttcatcgag atcgcgccat gccagcagta ccttaccaat cgcggtgctg
      781 tacagcggat tacgacgccc aatccgtgaa tacatgcgca aattgtacat agagtcaatt
      841 ttgtgaatgt aaacaatact gtcttcgtcc agtgcgccga ggtggatagt ttctttggtc
      901 aggcgggaga tctcacgcat ctggatatct gcgctacgaa ttaaatcgac gttttgtaac
      961 gcgcgagcgc ccagttcaaa caatttcagg gtcagggaat atttctccga ctccccttcc
     1021 tgcgccacat aacctaaggt tttcatggtc tgtaaaaagc gataaacggt gctttttgac
     1081 atcatgacgc gctgcgacag ctcggttatc cctatttcgc gctcttcacc cagcgcctgc
     1141 aaaatgccaa aaacttttag cacggaagat acagaatcag gctgtttatc cagatctgcg
     1201 ttagccattt atcacctcat tgcgagtgtt ttataaaaat cagaactgtt ttttattata
     1261 atttcgcacc agggtggtcg caatccatct tttgccggtt agttacaatt ctgcgacatc
     1321 caccgtgaat atcagtgcta gaatcatacc cctgttgatt attcaccaaa gatataaaat
     1381 tcctatgcca aaagttcagg ccgacggcct gccattgccc cagcgatacg gtgcgatatt
     1441 aaccattgtg attggtattt cgatggctgt ccttgacggc gcaatcgcca acgtcgccct
     1501 gccaacaatc gccacggacc ttcatgccac gccagccagt tccatctggg tagtgaacgc
     1561 ctatcaaatc gccattgtca tctccctgct ctcgttttcg tttctgggcg atatgtttgg
     1621 ctatcgacgt atttataaat gcggtctggt cgtttttctg ttgtcttcac tgttctgcgc
     1681 cctttctgat tcgctgcaaa tgctcaccct tgcgcgtgtc atacaaggtt tcggcggtgc
     1741 agcgttgatg agcgttaata ccgcacttat ccgcctgatc tatccacaac gttttctggg
     1801 gagagggatg ggcataaact cgtttattgt tgccgtctct tctgctgccg ggccgacaat
     1861 tgctgcagca atcctctcca tcgcatcctg gaaatggtta tttttaatca acgtaccgtt
     1921 aggtattatc gccctgcttc tggcgatgcg ttttctgcca cccaatggtt ctcgcgccag
     1981 taaaccccgt ttcgacctgc ccagcgccgt gatgaacgcg ttaaccttcg gcctgcttat
     2041 cactgcgttg agtggtttcg ctcaggggca atcgctgacg ttaattgctg cggaactggt
     2101 ggtaatggtt gttgttggta ttttctttat tcgccgccag ctttctcttc ccgtaccgct
     2161 gctaccggtg gatttactgc gtatcccgct gttttcactt tctatttgca catctgtttg
     2221 ctctttctgc gcacaaatgc tggcaatggt ttccctgccc ttttacctgc aaaccgtgct
     2281 cgggcgtagt gaagtcgaaa caggtttact tctgacaccg tggccgttag caacgatggt
     2341 gatggctccg ttggcaggct atttgattga acgcgtacat gcaggattgc tgggtgctct
     2401 agggttgttc atcatggctg cggggctttt ttccctggtt ctgctgcccg cgtcacctgc
     2461 ggatatcaat attatctggc cgatgatctt atgtggcgct ggatttggct tgttccagtc
     2521 acccaataac cacaccatta ttacctccgc tcctcgcgaa cgtagcggtg gagccagtgg
     2581 catgttagga acggctcgtc tactgggtca gagtagcggc gcggcactgg tggcgctgat
     2641 gctaaatcag ttcggtgata atggtacgca cgtttcgctg atggctgcgg ctattctggc
     2701 agtgattgct gcctgtgtca gtggtttacg tatcactcag ccacgatcca gggcataaaa
     2761 aaagcgcgtc gatcaggacg cgctttttag tatttacttc atattacttc aggtattcac
     2821 ccgtacgcag agcttcaatt cgtttatcca gcggcgggtg agtcatgaac aactcactga
     2881 gcgatttcga cttaccgtta atgcagaaag ccatcatgct ggttgcttct tgcggttcat
     2941 agctggtttt caggcgctgc agcgcggcaa tcattttctc gcgaccaacc agttttgccg
     3001 aaccggcatc agcatggaat tcacgatgac gcgagaacca catggtgata atgctcgcca
     3061 gaataccaaa caccagttcc agaaccgttg caaccgcaaa gtagatcagc gggttgccgt
     3121 tgctctcttc accttcatca cgatttccgc ccataaaacc cgcggcaagc tgcgccagaa
     3181 tacgggaaat aaagataacg aaggtgttca ccacgccctg aatcagcgtc atggtgacca
     3241 tatcaccatt ggcgatgtgg ctgatttcgt gagcaattac cgcctcggct tcatccgggc
     3301 tcatgttctg cagcaaaccg gtgctgacag caaccagaga ggcatcacgg cgcgcaccgg
     3361 ttgcaaaagc gttgatgtcc ggcgcatggt agatagccac ttgcggcata gcgatccctg
     3421 cctgacgagc ctgggttgct acagtattaa ccagccaacg ttccctttcg ttacgcggtt
     3481 gctcgatcac ttccccgcca acagatcgta atgccatcca tttggacatc agaagcgaaa
     3541 cgaaggaacc accaaaaccg aacagcaagg ccatgatcat cagcccctga acgctgctcg
     3601 actgtatccc tgtcaggctc agtaccagcc cgaaaacgac cattacggcc aggttcgtta
     3661 gcaggaagag cgcgattcgc atcataattt tctttttacc tcagtttaac aaaacgcaat
     3721 atgcgatacc cacatcgtat gggttacgcg actattttca agtctggata gtgcgtaagt
     3781 caccagaaag acacaacttt acattttgta gcatctgatt tacggcatct tgccgctgtt
     3841 aaaaaatcag gcacaatttc ttgtgcctga ttgatattac ttgacgggag cgggttgttc
     3901 cgcgggtctg gctttttcaa gcttcgccag atcgagtgcg atattcaccg tctcatccag
     3961 ataaggatcc ggctcctggt aatctttcgg tagatcatcc agcttcttca actccggttt
     4021 accttcgcgt ttaa
//
LOCUS       DAEVZA010000249         3906 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEVZA010000249 DAEVZA010000000
VERSION     DAEVZA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.249"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            75..815
                     /locus_tag="LAF92_005010"
     CDS             75..815
                     /locus_tag="LAF92_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBI9793889.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(786..1553)
                     /locus_tag="LAF92_005011"
     CDS             complement(786..1553)
                     /locus_tag="LAF92_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBI9793890.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1759..2337)
                     /gene="lpcA"
                     /locus_tag="LAF92_005012"
     CDS             complement(1759..2337)
                     /gene="lpcA"
                     /locus_tag="LAF92_005012"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBI9793891.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2577..>3906
                     /locus_tag="LAF92_005013"
     CDS             2577..>3906
                     /locus_tag="LAF92_005013"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBI9793892.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEK"
BASE COUNT          906 a         1011 c         1058 g          931 t
ORIGIN      
        1 atccagacta ttccgatttg ggatctattt tgccgtcctg gcgtgtgtaa ccgttttatc
       61 aaggaataag cagtatgcgt aaaatcgcat taattcttgc gatgttgttg atcccgtgcg
      121 tttcgtttgc cggtttgctg ggcagcagta gctcgaccac gccagtgagc aaagagtata
      181 agcagcagtt gatgggatcc cctgtctaca tccagatctt caaggaagaa cgtacgctcg
      241 atctctacgt caaaatgggc gagcaatatc agctgctcga cagttataaa atctgtaaat
      301 attccggcgg cttagggcca aaacagcgtc agggcgattt caaaagcccg gaagggtttt
      361 atagcgtcca gcgtaatcag ttaaaaccag acagccgtta ctacaaagcg attaatattg
      421 gtttccccaa tgcctatgac cgtgcgcatg gttatgaagg gaaatacctg atgatccacg
      481 gcgattgtgt ttccatcggc tgctacgcaa tgaccaatca gggtattgat gagatcttcc
      541 agttcgttac tggtgcgctg gtgtttggtc agccgagcgt gcaagtgagt atttacccgt
      601 tccgcatgac cgacgccaat atgaagcgcc ataaatattc caactttaag gacttctggg
      661 agcagctgaa gccgggctac gactactttg agcaaacccg taagccaccg accgtttctg
      721 tggtcaatgg tcgttacgtg gtcagcaagc cgttaagcca cgaagttgtg cagccacaac
      781 tggcatcaaa ctacacgctc cccgaggcaa aataagcgcc attcgcctgg cataatcttt
      841 tgccaggttt cattgcccgt cagcggctgt gttgcaatca ccgtgaccac atcattcggt
      901 gtggtctgcg agctgaagtc gatttccaca tcctgatcca gcagcgttgc cacgccaaac
      961 ggcgcgcggc gggtgatcca gtgtaaatta gtcgagcaat acgccatcac atagcgcccg
     1021 tcagaaagca gcatgttgaa aacgcccttc tgccgcagtt catccgccag tgaggcgata
     1081 tatttaaaca ctgccgccat gttgcccggt gtgcgcgggt aacgctgcgt taatttatgc
     1141 aggagccagc agaaggcttt ttcgctatcg gtttcaccga ccgggcggaa gttgccggtt
     1201 tccagtgatt tgtagcccgt cagttgtccg ttatgggcat aagtccagtt acgcccccat
     1261 aactcgcggg taaatgggtg agtattttcc agcgccacct cgccccgatt agcctggcga
     1321 atatgagcca ccaccgaaca ggattttatc gggtagtcct ggacaagttt ggcgatgggg
     1381 gaattaaagc tgggttgtgg atctttaaat gtgcgacagc ctttaccttc gtaaaaggta
     1441 atgccccagc catctttatg tggcccggtt ccaccaccac gctgtacaag tccggtgaaa
     1501 ctaaagcaga tatcggtagg gacgttggcg ctcatcccga gcaattcgca catgggtaac
     1561 ctccacaaca gcgggagcgc atggcccccg gcgaagattg atttaagaat tttcaggtcg
     1621 gatgcggcgt aaacgaatta ttcggcctac ggttcgcgta cagatttgta ggcatgataa
     1681 gacgcgtcag catcgcatca ggcatcagcg cacaagtgcc ggatgcggcg taaacgcctt
     1741 atccggccta cgccagactt acttaaccat ctctttttca attaactgga tcaggatatg
     1801 gatcacttta atgtgaatct cctgaatgcg gtcggcataa ccaaagtgcg gaacgcgaat
     1861 ttcgatatcc gccgtgccag ccattttacc gccgtcttta ccggtcaggg tgatcacttt
     1921 catccccttc tcacgcgccg ctgcgatcgc tttgatcacg tttgcagagt taccggaggt
     1981 ggagatcccc agcagtacat cgccttcgcg acctaccgct tcaacgtagc gggagaaaat
     2041 atcattgaaa ccgaaatcat taccgacgca ggaaatatga ctaacgtcag aaatagcaat
     2101 cgccgggtag cccggacggt tttcacggta gcgaccggtc aactcttcgg caaagtgcat
     2161 agcgtcgcaa tgggaaccgc cgttgccgca ggaaagcact ttgccaccgg ctttaaagct
     2221 gtctgctaac aggaccgccg cgcgctgaat ggcgtgaata ttggcgtcat cttttaaaaa
     2281 gttagccagc gtttccgccg cttcgttcag ttcgttacga ataagatcct ggtacatgag
     2341 gatatccttc agcataaatg taatagacaa aatgcagtgt accggatacc gccaaaagcg
     2401 agaagtacgg gcaggtgcta tgaccaggac tttttgacct gaagtgcgga taaaaacagc
     2461 aacaatgtga gctttgttgt aattatattg taaacacatt gctaaatgtt tttacatcca
     2521 ctacaaccat atcatcacaa gtggtcagac ctcctacaag taaggggctt ttcgttatga
     2581 tgattttgag tattctcgct acggttgtcc tgctcggcgc gttgttctat caccgcgtga
     2641 gcttatttat cagcagtctg attttgctcg cctggacagc cgccctcggc gttgctggtc
     2701 tgtggtcggc gtgggtactg gtgcctctgg ccattatcct cgtgccattt aactttgcgc
     2761 ctatgcgtaa gtcgatgatt tccgcgccgg tatttcgcgg tttccgtaag gtgatgccgc
     2821 cgatgtcgcg cactgagaaa gaagcgattg atgcgggcac cacctggtgg gagggcgact
     2881 tgttccaggg caagccggac tggaaaaagc tgcataacta tccgcagccg cgcctgaccg
     2941 ccgaagagca agcgtttctc gacggcccgg tagaagaagc ctgccggatg gcgaatgatt
     3001 tccagattac tcatgagctg gcggatctgc cgccggagtt gtgggcgtac cttaaagagc
     3061 atcgcttctt cgcgatgatt atcaaaaaag agtacggcgg actggagttc tcagcttatg
     3121 cccagtctcg cgtgctgcaa aaactctccg gcgtgagcgg gatcctggcg attaccgtcg
     3181 gcgtgccgaa ctcattaggc ccgggcgagc tgctgcaaca ttacggcact gacgagcaga
     3241 aagatcacta tctgccgcgt ctggcgcgtg gtcaggagat cccttgcttt gcactgacaa
     3301 gcccggaagc gggttccgat gcgggcgcga ttccggacac cgggattgtc tgcatgggcg
     3361 aatggcaggg ccagcaggtg ctggggatgc gtctgacctg gaacaaacgc tatattacgc
     3421 tggcaccgat tgcgactgtg cttgggctgg cgtttaaact ctccgacccg gaaaagctgc
     3481 tgggtggcgc agaagattta ggtattacct gtgcgctgat cccgaccacc acgccgggcg
     3541 tggaaattgg tcgtcgccac ttcccgctga acgtaccgtt ccagaacgga ccgacgcgcg
     3601 gtaaagatgt cttcgtaccg atcgattaca tcatcggggg gccgaaaatg gccgggcaag
     3661 gctggcggat gctggtggag tgcctctcgg taggccgcgg catcaccctg ccttccaact
     3721 caaccggcgg cgtgaaatcg gtagcgctgg caacgggcgc gtatgctcac attcgccgtc
     3781 agttcaaaat ctctatcggt aagatggaag ggattgaaga gccgctggcg cgtattgccg
     3841 gtaatgccta cgtgatggat gctgcggcat cgctgattac ctacggcatt atgctcggcg
     3901 aaaaac
//
LOCUS       DAEVZA010000250         3900 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEVZA010000250 DAEVZA010000000
VERSION     DAEVZA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.250"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..779
                     /gene="amyA"
                     /locus_tag="LAF92_005014"
     CDS             <1..779
                     /gene="amyA"
                     /locus_tag="LAF92_005014"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBI9793893.1"
                     /translation="KHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQTYIDQV
                     EGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQPLQAL
                     EAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDE
                     LILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGN
                     KTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(813..1226)
                     /locus_tag="LAF92_005015"
     CDS             complement(813..1226)
                     /locus_tag="LAF92_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI9793894.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            1413..2618
                     /gene="yedE"
                     /locus_tag="LAF92_005016"
     CDS             1413..2618
                     /gene="yedE"
                     /locus_tag="LAF92_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBI9793895.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2615..2848
                     /gene="yedF"
                     /locus_tag="LAF92_005017"
     CDS             2615..2848
                     /gene="yedF"
                     /locus_tag="LAF92_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBI9793896.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            2957..3628
                     /locus_tag="LAF92_005018"
     CDS             2957..3628
                     /locus_tag="LAF92_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBI9793897.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGKPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEISGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(3613..>3900)
                     /locus_tag="LAF92_005019"
     CDS             complement(3613..>3900)
                     /locus_tag="LAF92_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI9793898.1"
                     /translation="SGLNRSILDQGWYEMRRQLEYKQLWRGGQVFAVPPAYTSQRCAC
                     CGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGCQQTG"
BASE COUNT          878 a         1004 c         1036 g          982 t
ORIGIN      
        1 tcaaacatat accggcatgg ttttataaag agtggatcga acacgtacaa gaagttgcac
       61 caaagccgct gtttattgtg gcggagtact ggtcgcatga agtcgataaa ctgcaaacgt
      121 atattgatca ggtggaaggc aaaaccatgc tgttcgatgc gccgctgcag atgaaattcc
      181 atgaagcatc gcgcatgggg cgcgactacg acatgacgca gattttcacg ggtacattag
      241 tggaagccga tcctttccac gccgtgacgc tcgttgccaa tcacgacacc caaccgttgc
      301 aagccctcga agcgccggtc gaaccgtggt ttaaaccgct ggcgtatgcc ttaattttgt
      361 tgcgggaaaa tggcgttcct tcggtattct atccggacct ctacggtgcg cattacgaag
      421 atgtcggcgg tgacgggcaa acctatccga tagatatgcc aataatcgaa cagcttgatg
      481 agttaattct cgcccgtcag cgtttcgccc acggtgtaca gacattattt ttcgaccatc
      541 cgaactgcat tgcctttagc cgcagtggca ctgacgaata tcccggctgc gtggtggtca
      601 tgtcgaacgg ggatgatggc gaaaaaacca ttcatctggg agagaattac ggcaataaaa
      661 cctggcgtga ttttctgggg aaccggcaag agagcgtagt aaccgacgaa aacggtgaag
      721 cgaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa
      781 ttcatccccg gcggcaagcc ggggagattt cattacggca gtggcgtcgg taacggcgct
      841 ttatccagcg cggcggcaca ctctgccgtc gggcgatcca cacgctccat cgtcatacca
      901 tcgtactcaa tggtgttacc gtcacgctca acctcgtata gctcacgttt tacggtaacg
      961 ttagtgagat catcagacat caatgtcaac ttacccggca cggcaatcac ccgctgccac
     1021 tgacggcaat caagagtatc gccctcttta gtgacaatca ggctgcctat cgcttccggg
     1081 ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc ccgccagcca gtccggcgcg
     1141 ggggttttca cgacattgtt atagttttcg acttcggcac agcccgccag caacatgagc
     1201 gcgcctgcaa ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg
     1261 gcattaaagc cctgatgtcg ccagcctttc caggttcaga gagaaatttg atctaactat
     1321 tatttttgtg aattattacc ccgaaaataa atttgcatga tgtatgattc gcgactcttg
     1381 tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct
     1441 ggttgattaa attctgggca cccatccctg cggtcatcgc ggcgggtatt ctctccactt
     1501 actattttgg cattactggc accttttggg ctgtcacggg tgaatttacc cgttggggcg
     1561 gccagctcct gcagctgttt ggcgtccatg ctgaagagtg gggttatttt aaaattatcc
     1621 atctggaagg atcgccatta acccgcatcg acgggatgat gatcctcggc atgtttggcg
     1681 gctgctttgc cgcagcgctg tgggccaaca atgtcaaact gcgcatgcca cgcagccgta
     1741 tccgcattat gcaggccatt attggcggta ttatcgctgg ttttggcgcg cgtctggcaa
     1801 tgggctgtaa cctggcggcg ttctttaccg gtattcctca gttctcgctg catgcctggt
     1861 tctttgccat cgccactgcc attggttcat ggtttggcgc acgctttacc ctgctgccca
     1921 tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac
     1981 cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggt atgctgggct
     2041 gggcgctgct cacggcgatg aaccaaccaa aactggggct ggcaatgctg tttggcgtcg
     2101 gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt
     2161 ggatcaccgg acgtacccat atggcgaaag caatcattat cggtatggcg gtgagtgcca
     2221 tcgggatctt cagttacgta cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa
     2281 acgcggtaat tggtggttta ctgtttggtt ttggcatcgt gctggcaggc ggctgcgaaa
     2341 ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca
     2401 atgtgatcgg ctcgacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca
     2461 ccgactggga caaaatcaac ctgctgaaaa cctttggccc gatgggtggc ctgctggtga
     2521 catatttgct gttgtttact gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc
     2581 gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcattc ctgattaccg
     2641 tcttgatatg gtgggtgaac cctgccctta tccggcagtc gcaacccttg aggcgatgcc
     2701 gcagttaaaa aaaggtgaga tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa
     2761 tattccactg gatgcgcgta atcacggcta tacggtgctg gatattcagc aagacgggcc
     2821 aaccattcgt tatttaattc agaagtaatc cctcattccc gctgtggcca acagcgggaa
     2881 catcgttacc tctgtatcac atagatttta cccgccccct ctcctattct tttcagtatg
     2941 atctcaggag gttgctatgt gtggacgctt tgcccaatcc caaacgcgtg aagattacct
     3001 tgcgcttctc gcggaagata ttgaacgcga tattccctac gatcccgaac ccattggcag
     3061 atacaacgtc gcgccgggaa ccaaagttct tctgctcagt gaacgtgatg aacaccttca
     3121 tctggatccg gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctgattaa
     3181 cgcccgcgta gaaactgcgg ccaccagtcg tatgtttaaa ccgctctggc aacatggtcg
     3241 ggcaatctgt tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca aaaaacagcc
     3301 ttattttatc tatcgcgctg acggaaaacc tgtttttatg gccgcgatag gtagcacacc
     3361 gtttgagcgt ggtgacgaag ccgaaggatt tttaattgtc actgcagcag cagaccaggg
     3421 tctggtagat attcatgacc gccgcccgct ggtactgtcg ccagaagctg cacaggaatg
     3481 gatgcggcaa gagattagcg gtaaagaagc atcagaaatt gctaccaacg gctgtgtgcc
     3541 agcaaaccag ttcacctggc acccggtatc gcgcgcggta ggtaatgtca aaaaccaggg
     3601 cgcggagtta attcaacctg tttgttgaca tcctccacag gcaagaacgg cgtgccccgc
     3661 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
     3721 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
     3781 ctggcttgtg tacgctggcg gaacagcaaa cacctgaccg ccacgccaga gctgcttgta
     3841 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
//
LOCUS       DAEVZA010000251         3899 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEVZA010000251 DAEVZA010000000
VERSION     DAEVZA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.251"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1351)
                     /gene="aaeB"
                     /locus_tag="LAF92_005020"
     CDS             complement(<1..1351)
                     /gene="aaeB"
                     /locus_tag="LAF92_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBI9793899.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     "
     gene            complement(1357..2289)
                     /gene="aaeA"
                     /locus_tag="LAF92_005021"
     CDS             complement(1357..2289)
                     /gene="aaeA"
                     /locus_tag="LAF92_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBI9793900.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(2297..2500)
                     /locus_tag="LAF92_005022"
     CDS             complement(2297..2500)
                     /locus_tag="LAF92_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBI9793901.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            2683..3612
                     /gene="aaeR"
                     /locus_tag="LAF92_005023"
     CDS             2683..3612
                     /gene="aaeR"
                     /locus_tag="LAF92_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBI9793902.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(3746..>3899)
                     /gene="tldD"
                     /locus_tag="LAF92_005024"
     CDS             complement(3746..>3899)
                     /gene="tldD"
                     /locus_tag="LAF92_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="responsible for the proteolytic maturation of the
                     E. coli pMccB17 plasmid-encoded microcin B17, an exported
                     protein that targets the essential topoisomerase II DNA
                     gyrase; degrades the E. coli plasmid F-encoded CcdA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBI9793903.1"
                     /translation="IETMQQISMVGNDLKLDNGVGVCGKEGQSLPVGVGQPTLKVDNL
                     TVGGTA"
BASE COUNT          986 a         1022 c          988 g          903 t
ORIGIN      
        1 cttctatacc gaggaagaat cccagcactg ccaggctaat acacagcagc aacatgctct
       61 gttgggtatt agggataatc accaaaaagt agagcagccc taacggcagc gcggccagcg
      121 tcccgtagat aaagtcgatc gccaccatgc gtggattcgg caaacgcatt gccagtgacg
      181 tcacgaccgc aatcatcacc attgcaccac taccggaagt ccagcccgtc cacagccaga
      241 aaagcgttcc cagaatgcag gaaagtgtgg ttcgccagaa gttaaccatt gcatgatgac
      301 gttcggctga ctctactttt acttccggtt cgccttgcag gatctcttct tcggtggcgt
      361 tgatttttgt gttactgata acgccgcgct tgagaagctg ataacgcgtt gccgccgcga
      421 cccagctata aatggtgaca ggcgtttccc gttccccggt ccaggcgata actcttcgca
      481 ggcgtttgag ctgcttgtgg acgtcctgcg cggtttctac cggtgtgtca aaaaattcgc
      541 ggaaagtatc agtgatcaat tccgggcgcg tattctgaat aagataagtt tcgcaggatt
      601 gggtaatcag cgtcagcgat agcgtattga tcgcttttaa acgtcgattg gcccgcgccc
      661 agcgggaaga ttccatattc aggttgctgc gcataccttg tagcgccgtg gtgcgtcgaa
      721 ccaggtcgcc ccaggcttta tcgacaactt caccatcgcc atgcttgata cagagttgca
      781 ttaattgata ttgcgcgacc agcaaacttt ccagctctcg atccacttct tgtttgatcg
      841 atcgcggaga aaagagcaaa tccgccataa ttgcacacac aataccgata acgatctcgc
      901 tacaacgttc gacggcaaac tgcggcgtaa gcaatggttc aggctgaatg gtgatcacaa
      961 tgatcagcgc ggtataaccg gccagccccc acgcatacga gttttctatt cgtaccagcg
     1021 aggatatcca ggtacaaaaa ccggcccaga tacagcacac cagaatcatc aataatggtg
     1081 cgcggatcat cgcaatgatg atcaccagtc cggcaataca gccaataaat gtgccgatga
     1141 tgcgcaaaaa gccacgatag cgaatagcgc cagaatacgg ttcacctccc gcagcaaagg
     1201 ccggaccggc agcaacaatc gccgctgtca gtaccgccca gcgtggcgtt tccagctgga
     1261 agtgaaagcc aacaaacagc gccagtacaa tggcggtcgc cagttttacc gcaaagcgaa
     1321 tatgttggtt agcaatggag aaaataccca tcgtgattaa ccaaactcac gcaggcgatg
     1381 ggccatttta cggaagaacg aatcctggct ttcgtcgcga tcttgtttgc cagtgaccac
     1441 cactgtagca gtggtgcccg caggccagat gttctcttgc tggttgtcga gacgaatacg
     1501 aaccggaaca cgttgcgcca aacgcaccca ttcaaggtta gagtcgatgg tcgccatccc
     1561 tttgtcgtca cgcgtgctgc tggcgttggt gaccccagcg gcaacactat caacagtccc
     1621 tttcagcact ttgttactgc caagcggcgt gatctctgca cgatacccag ggcgcacccc
     1681 ttccagctta gtttcttcca tataggccag tacatagaaa gagttctgtt tcaccagcgc
     1741 aaccgccgtt gatccacgag taataaactc accggtatag acgttgaggt tggtcaccca
     1801 gccatccgct ggcgcgcgga tcaccgtgcg ttcaagatcc agttttgcca gatcgcgggt
     1861 cgcctgcgct ttcgctaact gatgcagaac cgtttgtagt acgttgttgg cctggtcgat
     1921 ctcttcgcga gacatcgcct gcacaccgag acgattacga cgtccggcct cctggcgttt
     1981 ctcctgtgcc agtacctgat aataagcaac atcggcttgc gcttcctcaa gcgccttttg
     2041 atagcgcggc tggtcgatgg tgaacagtac ctgtcctttt ttcaccagct ggttatcatg
     2101 aacattcacc tgggtaatga gtccagaaac gtccggcgcg atcgcaacga catcagcgct
     2161 aaagcgcgcg tcacgcgtcc acggggattc ggtgtaatag acccaggcat taaaaattgc
     2221 gatgaaggcc agaatgacta atacgaccgt gatggccgta cgggagaatt ttcttattag
     2281 tgttttcact tcaacctcaa acgaacagtc gcgatatcaa ataaaacaag cagcaataga
     2341 gcgcggtgtt gaacaacgcc ggatgccaga caaagtcgta gatacctgtt ggcacaagta
     2401 cccggcgcac cagccagaaa atcgccagtg ataaaagcaa ttcaaaaaat atcggtggga
     2461 aggacagccc aaacaccacg ataacgggaa acagactcat gtcgaccttg gttgtataga
     2521 gagagcagga gttattattt tcagcatctg tcgccgcaga gaagggcatg gaaagccggg
     2581 cgagagcaac attgctgtag attgatattt aatatattag cgtaactgtt atgctgttat
     2641 ctatattatg tgatctaaat cacttttaag tcagagtgaa taatggaacg actaaaacgc
     2701 atgtcggtgt ttgccaaagt agttgaattt ggctctttta ccgccgccgc cagacagcta
     2761 cagatgagcg tttcgtccat cagtcagacg gtgtcaaaac tggaagatga gttgcaggta
     2821 aagctgttaa accgtagtac acgcagcatt ggtctgactg aagccggcag aatttactac
     2881 cagggctgcc gtcggatgct ccatgaagtg caggatgttc atgagcaact gtatgccttc
     2941 aataacaccc ccatcgggac gctgcgcatt ggctgttctt caactatggc acaaaatgtt
     3001 ctcgccgggc tgacagcaaa aatgctgaaa gaatacccag gtttgagcgt caatctggtt
     3061 accggaattc ctgcccccga cctgattgcc gatggtctgg atgtggtaat ccgcgtcggc
     3121 gcgttgcagg attccagcct gttttcccgc cgtctgggcg cgatgccgat ggtggtgtgc
     3181 gccgcgaaaa gctatctcac acaatacggc ataccggaaa aacccgccga tttgagtagt
     3241 cattcatggc ttgaatacag cgtgcggccc gacaatgaat ttgaactgat cgcaccggaa
     3301 gggatctcga ctcgcctgat cccacaagga agatttgtga ctaatgatcc gatgacgctg
     3361 gtgcgctggc tgacggcggg tgccgggatc gcctacgtgc cgctgatgtg ggtgatcaac
     3421 gagatcaatc gtggggagct ggagatcctg ctgccgcgtt accagtcaga tccacgcccg
     3481 gtttatgcgt tgtataccga aaaagataaa ctgccgctga aggtacaggt cgtgatcaac
     3541 tcgctgacgg attattttgt tgaggtcggt aaattgtttc aggagatgca tgggcgcggg
     3601 aaagagaagt aattcatatt gtactgttac gttgttcaaa cctgtgccaa cgggttcccc
     3661 tcaccctaac cctctcccca aaggggcgag gggaccgttc gtgcacgtag aaagattaat
     3721 tatccttctg aaaatagtga aattattacg cagtaccgcc aacagtcagg ttatcgactt
     3781 tcaacgttgg ctggcccacg ccaaccggca aactttgccc ttctttaccg cagacaccca
     3841 cgccgttatc cagtttcagg tcgttgccaa ccatcgaaat ctgctgcatg gtttcgata
//
LOCUS       DAEVZA010000252         3872 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEVZA010000252 DAEVZA010000000
VERSION     DAEVZA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.252"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            177..1271
                     /locus_tag="LAF92_005025"
                     /pseudo
     CDS             177..1271
                     /locus_tag="LAF92_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1353..1757
                     /locus_tag="LAF92_005026"
     CDS             1353..1757
                     /locus_tag="LAF92_005026"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBI9793904.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            1754..2101
                     /gene="tnpB"
                     /locus_tag="LAF92_005027"
     CDS             1754..2101
                     /gene="tnpB"
                     /locus_tag="LAF92_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI9793905.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            2150..3685
                     /locus_tag="LAF92_005028"
     CDS             2150..3685
                     /locus_tag="LAF92_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HBI9793906.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALQEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPLARS
                     TAD"
BASE COUNT          974 a         1043 c         1052 g          803 t
ORIGIN      
        1 ttcttatccg caatcaagcc catgcggaaa gagagcgtga aatagaagaa atgacgcaaa
       61 agatccgggc tgttttcacg gatcacactg agctgaactc tctgatagac catctacagg
      121 aactcagtaa tgctttcaaa tcaaccagtt ccgggatttc acgttcttca accggcatga
      181 gaggtctgtc aggcggaaat aaaatacacc acattccggc tggtcttgag aactatcaac
      241 cctacatccg cagtgaacgc cgcgtggaat ggattgactg gcagacaaag ggactcgagt
      301 tttcgcccct ttcggatggc tgctgtccgt tctgtaccgg tgatatcacg ggaaaggaag
      361 cacaaattcg ccaagtcaga gaggaatacg ataaatccac cattaaaaac ctgaccgcta
      421 tcatcagact ggtggaaaac ctcggtaatt acctgacgga gagcgccaga gaacgccttc
      481 tggccattac tatgcttcag aacggtccgg aagccgaaca tatcgagtac ctagtggcgt
      541 tgaaacgcca gaccgatacg ctgacagaga aactcactgc gctgagaggc ctgaatgttt
      601 tcagcctgca ggaacagcag aacgttcggg aggtgctcac tgccaggttg attgacctgc
      661 agttcttccc tgatctgcaa tccgaactca tgcaggggat caccgacaga ctgaacgcgg
      721 cccttatgga cctgataaac cttgccggac cgctgcaggg aaaaattaac cggcaccggg
      781 acagcatgat ccggctgatc gcacagcaca aaacaaacat caataatttc ctcacttatg
      841 caggctataa atacagggtg gatatagccg gcgagggaga gcagagaaaa ctcaggctac
      901 gacacataga ttttgacggg tacgtcagcg gcggtagcca gcatctcagc tatggagaac
      961 ggaacgcctt tgcgattgtg ttattcatgt atgaatgcct gtcaaaaaac ccgggactga
     1021 ttatccttga tgatcccata tcttcttttg ataaaaacaa gaagtttgcc attcttgaaa
     1081 tgctgttcag acgtgcaagt ggtgaatgcc tgaagaaccg gactgtactt atgctgacgc
     1141 atgacgtgga gcctgtgatt gacacgctga agtcagtcag aaggttgttc agtaatcagg
     1201 tgaccgcctc ctgcctgcgc ctgtctgctg gagtcataga agaattacct gttaacgacg
     1261 gcgatatcat ggtaagcgtc gtctcagcac cgtctggcag atcctgaaat tcctgagaga
     1321 atagtggaca ccaaatatgg tggacgcaat ccatgaaatc attaaccgca gtgcgtaaaa
     1381 aaagccctaa ttatcccgtt gagttcaaaa tcaaaatggt tgaactctcg catcgaccag
     1441 agatctccgt agcgcaactc gctcgtgagc atgggatcaa cgataatttg ctgttcaagt
     1501 ggcgccagta ctggcgcgaa ggaaaactac gtcctccttc aacaacagaa aacaacgtgc
     1561 ctgagctgct cccgataaca cttgatgccg aagatgttgt ccctacaacc tccccccggt
     1621 cacaacctgt agctgctgcg acacctgaat cactcaatat cagctgtgaa gtgacgttcc
     1681 ggcacggatc actccgtctg aatggtgcca tcagcgaaaa tatcctgaac ctgctgatac
     1741 gggagctcaa acgttgatcc cattaccatc agggacaaag atctggctgg tcgctggcat
     1801 caccgatatg agaaacggct tcaacggcct ggcggcaaag gtgcagacga cgctgaaaga
     1861 cgatccgatg tcaggtcacg tttttatctt ccgtgggcgt aatggcagtc aggtaaagct
     1921 cctctggtct accggcgatg gactgtgtct gctgaccaaa cggctggagc gcggccgctt
     1981 cgcctggccg tcagcccggg atggcaaagt gttcctcaca ccggcacagc tggcgatgct
     2041 gctggaaggt atcgactggc ggcagcctaa aagactgctt acgtccctga ctatgttgta
     2101 agcctcttta tcctggtcga cgctgaatga gcctggtaat atacccggta tgagcagctc
     2161 acttcctgac gatatcaatg cactgaaacg tctccttgcc gaacaggagg cgctgaaccg
     2221 tgccctgcag gaaaagctga acgagcgtga acgcgaaata gaccatctgc aggcacagct
     2281 ggataagctg cgccggatga acttcggcag ccgctcggaa aaagtctccc gtcgtatcgc
     2341 acagatggaa gctgacctga aggcacttca gaaagaaagt gataccctta ccggtcgggt
     2401 tgacgacccg gccgtgcagc gcccgctgcg tcaaacccgc acccgcaaac cgttccccga
     2461 atcactcccc cgcgatgaaa aacggctgct gccggcagcg tcatgctgcc cggaatgtgg
     2521 aggctcgctg agctatctgg gtgaggatgc cgccgaacag ctggagctga tgcgcagcgc
     2581 cttccgggtt atccggactg tacgtgaaaa gcatgcctgt actcagtgcg atgccatcgt
     2641 gcaggccccc gcgccttcac ggcccatcga gcggggtatc gcaggaccgg ggctgctggc
     2701 ccgcgtgctg atctcaaagt atgcagagca caccccgctg taccgccagt ctgaaatgta
     2761 cggccgccag ggcgtggagc tgagtcgttc actgctgtcg ggctgggtgg atgcatgctg
     2821 ccggctactg tcaccgctgg aagaagcgct tcaggactat gtgctgactg acggtaagct
     2881 ccatgctgat gacacgcctg tcccggtgct gttgccaggc aataagaaaa cgaagaccgg
     2941 gcggttatgg acctacgttc gtgacgaccg taacgccggg tcaacgctgg cgccggcggt
     3001 gtggttcgct tacagcccgg acagaaaagg catccatccg cagacccatc ttgcggggtt
     3061 cagtggtgta ctgcaggcgg atgcatacgc cgggttcaac gagctgtacc gggatggccg
     3121 gataacggaa gccgcctgtt gggctcacgc ccgccgtaaa atccacgatg tgcacgttcg
     3181 caccccgtca gccctgacgg aggaagcgct gaaacggatc ggcgaactgt acgccatcga
     3241 ggcagagata aggggaatga cggcggagca gcgccttgcc gaacgtcagt tgaaaacgaa
     3301 accgctgctg aaatccctgg aaagctggct gcgtgaaaag atgaaaaccc tgtcgcgaca
     3361 ctcagaactg gcgaaagcgt tcgcatacgc cctgaaccag tggccggcgc tgacgtacta
     3421 tgcagatgat ggctgggctg aggcggacaa taacatcgct gaaaatgcgt tgcggatggt
     3481 cagtctgggc cgcaaaaact acctgttctt cggttcggat catggaggag agcggggagc
     3541 gctgctgtac agcctgatcg ggacgtgcaa actgaacgga gtggagccag aaagctacct
     3601 ccgctatgtc cttgacgtca tagccgactg gccgataaac cgggtcggcg aactgctccc
     3661 cctggcgcgt agcactgccg actgaataac acatccccgt caatacggtt cttgctgcac
     3721 gcttacatat catgacattc atgcagatct gcaaatccat caccgcgtca gcagactgtg
     3781 aggagatcat taagctcatc tatcttcgtc gctactttga aatcgttgac gaacgtggtg
     3841 atgcttatca gcttctatcc aatctattcc at
//
LOCUS       DAEVZA010000253         3700 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEVZA010000253 DAEVZA010000000
VERSION     DAEVZA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.253"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            2..394
                     /locus_tag="LAF92_005029"
     CDS             2..394
                     /locus_tag="LAF92_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBI9793907.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            532..1416
                     /locus_tag="LAF92_005030"
     CDS             532..1416
                     /locus_tag="LAF92_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBI9793908.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="LAF92_005031"
     CDS             complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="LAF92_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HBI9793909.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            2753..3403
                     /gene="tetR(A)"
                     /locus_tag="LAF92_005032"
     CDS             2753..3403
                     /gene="tetR(A)"
                     /locus_tag="LAF92_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBI9793910.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(3435..3677)
                     /locus_tag="LAF92_005033"
     CDS             complement(3435..3677)
                     /locus_tag="LAF92_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HBI9793911.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
BASE COUNT          686 a         1144 c         1162 g          708 t
ORIGIN      
        1 cgtgctgttc cccaactttt cgctcggcga agagtatgaa catgcgccgc cggcgacgaa
       61 tcgccaaatc tcaccgtatc tcccgagcgg acgatttcgc accggtctgc ccgtcgaagg
      121 gcttgcgatc gaacggggcg accttttcta tgcatgtccg cgagccagcg tcttttatgg
      181 cacggcgctc gacgccgacc ttcggacgcg cggcgtaagc acgcttgtca tggccgggat
      241 aagcaccacc ggcgttgttc tttcaagcgt cgcctgggct agtgatgcgg actacgacgt
      301 gcgtttggtc caggactgct gctacgaccc ggatcgggat gcccacgaag ctctgttgcg
      361 ttccgggttc ggcggacgtg tacaggtcgt gtaattttcg ggcctcgcat gatcgcggcc
      421 atagccgcag gtggattggt gcgaggcagg gccagtccaa gtcagggtgc ggtcatcgcg
      481 gcgcctgcaa ctctcaaaaa cccatcgccg tagcggaggt cttgtaatcg catgtcatta
      541 cgcacgcccg atttattgtt cacagcgata gcacctgcca tttggggcag cacctacatt
      601 gtcaccaccc aatacctgcc gaacttctca ccgatgacgg tcgcgatgct gcgggcgttg
      661 ccggcgggtt tattgctcgt gatgatcgtc cgacagattc caacgggaat ctggtggatg
      721 cgcatcttca tcctcggcgc acttaatatt tcgctattct ggagcttgtt gtttatttcg
      781 gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc
      841 gtgttcatct ctgccgctct gctaggtagc ccgatacgat tgatggcggt cctgggggct
      901 atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc gctagatcct
      961 gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc
     1021 cgcaagtggc aacctcccgt gcctctgctc acctttaccg cctggcaact ggcggccgga
     1081 ggacttctgc tcgttccagt agctttagtg tttgatccgc caatcccgat gcctacagga
     1141 accaatgttc tcggcctggc gtggctcggc ctgatcggag cgggtttaac ctacttcctt
     1201 tggttccggg ggatctcgcg actcgaacct acagttgttt ccttactggg ctttctcagc
     1261 ccggggaccg ccgtgttgct aggatggttg ttcttggatc agacgctgag tgcgcttcaa
     1321 atcatcggcg tcctgctcgt gatcgggagt atctggctgg gccaacgttc caaccgcact
     1381 cctagggcgc gtatagcttg ccggaagtcg ccttgacccg catggcatag gcctatcgtt
     1441 tccacgatca gcgatcggct cgttgccctg cgccgctcca aagcccgcga cgcagcgccg
     1501 gcaggcagag caagtagagg gcagcgcctg caatccatgc ccacccgttc cacgttgtta
     1561 tagaagccgc atagatcgcc gtgaagagga ggggtccgac gatcgaggtc aggctggtga
     1621 gcgccgccag tgagccttgc agctgcccct gacgttcctc atccacctgc ctggacaaca
     1681 ttgcttgcag cgccggcatt ccgatgccac ccgaagcaag caggaccatg atcgggaacg
     1741 ccatccatcc ccgtgtcgcg aaggcaagca ggatgtagcc tgtgccgtcg gcaatcattc
     1801 cgagcatgag tgcccgcctt tcgccgagcc gggcggctac agggccggtg atcattgcct
     1861 gggcgagtga atgcagaatg ccaaatgcgg caagcgaaat gccgatcgtg gtcgcgtccc
     1921 agtgaaagcg atcctcgccg aaaatgaccc aaagcgcggc cggcacctgt ccgacaagtt
     1981 gcatgatgaa gaagaccgcc atcagggcgg cgacgacggt catgccccgg gcccaccgga
     2041 acgaagcgag cgggttgaga gcctcccggc gtaacggccg gcgttcgcct ttgtgcgact
     2101 ccggcaaaag gaaacagccc gtcaggaaat tgaggccgtt caaggctgcc gcggcgaaga
     2161 acggagcgtg gggggagaaa ccgcccatca gcccaccgag cacaggtccc gcgaccatcc
     2221 cgaacccgaa acaggcgctc atgaagccga agtgccgcgc gcgctcatcg ccatcagtga
     2281 tatcggcaat ataagcgccg gctaccgccc cagtcgcccc ggtgatgccg gccacgatcc
     2341 gcccgatata gagaacccaa aggaaaggcg ccgtcgccat gatggcgtag tcgacagcag
     2401 cgccggccag cgagacgagc aagaccggcc gccgcccgaa acgatccgac agcgcgccca
     2461 gcacaggtgc gcaggcaaat tgcatcaacg catacagcgc cagcagaatg ccatagtggg
     2521 cggtgacgtc gttcgagtga accagatcgc gcaggaggcc cggcagcacc ggcataatca
     2581 ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat tacgatcagg ggtctgttgg
     2641 gtttcacgtc tggcctccgg accagcctcc gctggtccga ttgaacgcgc ggattcttta
     2701 tcactgataa gttggtggac atattatgtt tatcagtgat aaagtgtcaa gcatgacaaa
     2761 gttgcagccg aatacagtga tccgtgccgc cctggacctg ttgaacgagg tcggcgtaga
     2821 cggtctgacg acacgcaaac tggcggaacg gttgggggtt cagcagccgg cgctttactg
     2881 gcacttcagg aacaagcggg cgctgctcga cgcactggcc gaagccatgc tggcggagaa
     2941 tcatacgcat tcggtgccga gagccgacga cgactggcgc tcatttctga tcgggaatgc
     3001 ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc gcgcgcatcc atgccggcac
     3061 gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag cttcgcttcc tctgcgaggc
     3121 gggtttttcg gccggggacg ccgtcaatgc gctgatgaca atcagctact tcactgttgg
     3181 ggccgtgctt gaggagcagg ccggcgacag cgatgccggc gagcgcggcg gcaccgttga
     3241 acaggctccg ctctcgccgc tgttgcgggc cgcgatagac gccttcgacg aagccggtcc
     3301 ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat ggattggcga aaaggaggct
     3361 cgttgtcagg aacgttgaag gaccgagaaa gggtgacgat tgatcaggac cgctgccgga
     3421 gcgcaaccca ctcactacag cagagccatg tagacaacat cccctccccc tttccaccgc
     3481 gtcagacgcc cgtagcagcc cgctacgggc tttttcatgc cctgccctag cgtccaagcc
     3541 tcacggccgc gctcggcctc tctggcggcc ttctggcgct cctgctgcgg cgtccgctcg
     3601 tgggccgtgg cgcgggtccg cgcgccggcc tcgtgcgcct ggcgctcgcg ggcgaggtcc
     3661 agggcggccg tcttcacgtt ctgccttgcg cagatgagat
//
LOCUS       DAEVZA010000254         3660 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEVZA010000254 DAEVZA010000000
VERSION     DAEVZA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.254"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            2..>3660
                     /gene="traI"
                     /locus_tag="LAF92_005034"
     CDS             2..>3660
                     /gene="traI"
                     /locus_tag="LAF92_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBI9793912.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRTEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISHQ
                     KSALHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFTELGLEIDAQIKR
                     GDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPSELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRT"
BASE COUNT          815 a          959 c         1221 g          665 t
ORIGIN      
        1 gatgatgagt atcgcgcagg tcagatcggc cggaagtgcc ggtaactatt acaccgacaa
       61 ggataattac tatgtactgg gcagcatggg agaacgctgg gccggcaagg gggctgaaca
      121 gcttgggctg cagggcagcg tcgataagga tgtttttacc cgtcttctgg agggaaggct
      181 gccggacgga gcggatctaa gccgcatgca ggatggcagt aataagcatc gtcccggcta
      241 cgacctgacc ttctccgccc ccaaaagtgt ctccatgatg gccatgttag gtggcgataa
      301 gcgcctgatt gatgcacata accaggccgt ggactttgct gttcgtcagg tggaggcgct
      361 ggcctccaca cgggtgatga cggacggaca gtcagaaacg gtgctgaccg gtaatctggt
      421 gatggcactg tttaaccacg acaccagtcg cgatcaggaa ccacagttac acacgcatgc
      481 ggtggtggct aatgtcacgc agcataacgg tgagtggaag acactgagca gtgacaaagt
      541 ggggaaaacg gggttcattg agaatgtgta cgctaatcag attgcctttg gcaggctcta
      601 ccgggaaaaa ctgaaagagc aggttgaggc gctgggctat gaaaccgaag tggtgggtaa
      661 gcacggtatg tgggagatgc cgggcgtacc ggtggaggcc ttttccggac gctcacaggc
      721 tatccgggag gccgtcgggg aagacgcctc gctgaaatcc cgggatgtgg cggcactgga
      781 tacgcgtaaa tccaaacagc acgtcgatcc ggaaatcaga atggctgaat ggatgcagac
      841 gctgaaggaa accgggttcg acatccgggc atatcgtgac gcggcggatc agcgtacgga
      901 gatccgcacg caggcgcccg ggccagcttc acaggacggg ccggatgtgc agcaggcggt
      961 gacacaggcg attgccggat taagtgaacg aaaagtgcag ttcacgtaca cggacgtgct
     1021 ggccaggacg gtcggcatac tgccgccgga aaatggtgtg attgaacggg cacgcgccgg
     1081 tatcgatgag gccatcagcc gtgagcagct tatccccctc gatcgtgaga aggggctgtt
     1141 cacgtccggg attcatgtgc tcgatgagct gtcagtccgg gcactcagtc gtgacatcat
     1201 gaaacagaac cgggtgaccg tacatccgga gaaaagtgtc ccccggacgg ccggttacag
     1261 cgatgccgtc agtgttctgg cacaggatcg cccgtcgctg gccattgtgt ccgggcaggg
     1321 cggtgcagcc gggcagcgtg agcgggtggc tgaactggtc atgatggccc gggaacaggg
     1381 gcgggaggtg cagattatcg ctgctgaccg tcgctcgcag atgaacctga agcaggatga
     1441 acggctgtcc ggtgaactga taaccggacg tcgtcagctg ctggaaggca tggccttcac
     1501 gccgggcagt accgttatcg tcgaccaggg cgaaaaactc tccctgaaag agacgttaac
     1561 cctgctggat ggtgccgcac gtcataacgt acaggtcctg ataaccgaca gcgggcagcg
     1621 aaccggtaca ggcagtgcgc tgatggccat gaaggatgcc ggggtgaaca catatcgctg
     1681 gcagggcgga gaacagcgac cggccaccat catcagtgaa ccggaccgta atgtccgcta
     1741 tgcccggctg gccggagatt ttgcggccag cgtgaaagcc ggagaagaga gcgtggcaca
     1801 ggtcagcggg gtacgggaac aggccattct gacacaggcc attcgcagtg agctgaaaac
     1861 acagggcgtg ctcggacacc cggaggtaac catgaccgcc ctgtcaccgg tctggctgga
     1921 cagccggagc cgttatctgc gggatatgta ccgtccgggg atggtgatgg agcagtggaa
     1981 cccggagaca cgcagtcatg accgctatgt tatcgaccgg gtgacggcgc agagtcacag
     2041 cctgaccctg cgggatgcgc agggcgaaac gcaggtggta cgtatttctt ctctggacag
     2101 cagctggtcg ctgttccggc cggaaaaaat gccggtggca gacggcgagc gtctgagggt
     2161 gacagggaaa attcccggac tccgcgtctc cggcggtgac cgcctgcagg tggcatccgt
     2221 cagtgaagat gcgatgacgg ttgttgtgcc ggggcgggct gaaccggcct ccctgcctgt
     2281 gagcgattca ccattcacgg cactgaagct ggagaacggc tgggtggaaa cgcccgggca
     2341 ttccgtcagc gacagtgcga aggtttttgc ctccgtcaca cagatggcaa tggacaacgc
     2401 caccctgaac ggtctggccc gcagcggtcg tgatgtccgg ctgtattctt cactggatga
     2461 aacccgtact gcggaaaaac ttgcccgcca tccctccttt acggtggttt ctgagcagat
     2521 aaaggcgcgt gccggtgaga catcgctgga aacggctatc agccatcaga agtccgcact
     2581 tcacacgccg gcacagcagg ccattcatct ggcccttccg gtggtggaaa gtaaaaacct
     2641 ggccttcagc catgtggacc tgctgacaga ggcgaagtcg tttgctgcag aaggaaccag
     2701 ttttactgaa ctggggctgg aaatcgatgc gcagataaaa cggggtgatt tactgcatgt
     2761 ggatgtggct aaaggctatg gcacagacct gctggtttcc cgtgcgtcgt atgaggctga
     2821 aaaaagcatt cttcgccata ttctcgaagg taaggaggcg gtcacgccgc tgatggagag
     2881 agtacctagc gaactcatgg agaaactgac atcaggacag cgcgccgcca cccgcatgat
     2941 actggaaacg tccgaccgtt tcacggtggt gcagggctat gccggtgtgg ggaagaccac
     3001 acagttccgg gcggtgatgt cagccgtgaa catgctgccg gagagtgagc gtccccgcgt
     3061 cgtggggctg ggtcccacac accgtgcggt cggggagatg cgcagcgccg gcgtggatgc
     3121 gcagacactg gcgtcatttc tgcatgacac gcagctgcag cagcgcagcg gagaaacgcc
     3181 ggatttcagc aacacgctgt tcctgctcga tgagagctca atggtgggca ataccgacat
     3241 ggcacgggca tacgccctga ttgcggccgg tggcggtcgt gccgtggcca gtggtgacac
     3301 ggaccagttg caggccatcg cgcccggtca gcctttccgt ctccagcaga cgcgcagtgc
     3361 tgccgatgtg gccatcatga aggagattgt gcgccagacg ccggagctgc gggaggcggt
     3421 atacagcctg attaaccggg atgtggaaag ggcactgtcc gggcttgaga gtgtgaaacc
     3481 gtctcaggtg ccacgtcagg agggcgcatg ggtaccggag cactccgtga cggagttcag
     3541 tcacagccag gaagcgaaac tggcagaagc gcagcagaag gcgatgctga aaggtgaggc
     3601 ttttccggat attcccatga cactgtatga agccattgtc cgcgattata ccggcaggac
//
LOCUS       DAEVZA010000255         3636 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEVZA010000255 DAEVZA010000000
VERSION     DAEVZA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.255"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            122..1597
                     /locus_tag="LAF92_005035"
     CDS             122..1597
                     /locus_tag="LAF92_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI9793913.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1778..3268
                     /locus_tag="LAF92_005036"
     CDS             1778..3268
                     /locus_tag="LAF92_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI9793914.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3311..>3636
                     /gene="yeaK"
                     /locus_tag="LAF92_005037"
     CDS             3311..>3636
                     /gene="yeaK"
                     /locus_tag="LAF92_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBI9793915.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGA"
BASE COUNT          987 a          787 c          767 g         1095 t
ORIGIN      
        1 tggctgattt ttagcttact gtaaattatc tcatttatta tatacattag cttacaatcg
       61 ctttaaatat gacagcataa cctttacata atttagttcc agaaaacaat cattcggaaa
      121 aatgattcag tcaacacgta tttccatggg gttattcttt aaatattttt tatcgttaac
      181 gaaaattgat cctggtcaaa actatatatc tctgccatca ataaaatcca gcactcacat
      241 tgctctcctt tttatggttt ctatgggtac acaaaaatta aaagctcaaa gcttttttat
      301 tttcagttta ttgctgacgt taattttatt ttgcatcacc acattatata acgaaaacac
      361 aaatgtaaaa ctcatcccac agatgaatta cctgatggtt gttgtggctt tgtttttcct
      421 taacgccgtc atttttcttt tcatgttaat gaaatatttc actaacaaac aaattttacc
      481 aacactcatt ttaagccttg catttttaag tggccttatc tatttagttg aaaccattgt
      541 aattatccat aaaccaatta acggcagtac actgatccag acaaagtcga atgatgtttc
      601 tattttctat attttccgcc aactcagttt tatttgttta acctcgctgg cactcttttg
      661 ttatggaaaa gacaacatcc ttgacaacaa taagaaaaaa acgggaatcc tgttgctggc
      721 gctgattcct tttttagttt ttccccttct ggcacacaat ctgagcagtt ataacgctga
      781 ctattctttg tatgtcgtcg actactgtcc ggacaaccat actgcgacct ggggaatcaa
      841 ctatacaaaa atattggttt gtctgtgggc atttttactg ttctttatta tcatgcgtac
      901 acgattagcc agcgaactat ggccgttaat agcattatta tgtctggcat cgctatgctg
      961 caacttactt ctactgactc tggatgagta taattacacc atctggtata tcagtcgcgg
     1021 gattgaagtt tccagtaaac tgtttgttgt gtcttttctg atttataaca tttttcagga
     1081 actgcaactc tccagcaaac tggcagttca tgatgtgctg accaatattt ataatcggcg
     1141 ctactttttc aacagcgtag agtcattatt gtcgcgacct gttgttaagg acttctgtgt
     1201 catgctggtt gatattaatc agttcaaacg catcaatgcc caatggggac atcgtgtggg
     1261 tgataaagtg ctggtttcaa ttgtcgatat tatccagcaa agcatccgcc ctgatgatat
     1321 tttagcgcga ctggagggtg aggtgtttgg cttgctattt accgaactca atagtgccca
     1381 ggcaaaaatc attgcggaac gtatgcgtaa aaatgtcgaa ctcctgaccg gctttagtaa
     1441 cagatatgat gttcctgaac aaatgaccat cagtattggc acggtttttt caacgggtga
     1501 cacgcgtaat atctcgcttg tcatgacgga agcagataaa gccttacgcg aagcgaaaag
     1561 cgaggggggc aacaaagtga ttattcatca tatttaagtg caaaaatatt cagagccatg
     1621 ctttttgcgt ggcttttgca tacaatttat tactattacc cctaaatttc ccctccccag
     1681 cggcgcagag atgagtataa ttagcgcccc tgtgccaggc cgcaatcgaa ctttatctgg
     1741 ttttctcgtt tcactaaccg aaggagtgcc atttatcatg aaattgcacc atagaatgct
     1801 ccggcatttt atcgccgcaa gtgtcattgt gctgacatct tccttcctta tttttgaact
     1861 tgtcgccagc gacagagcaa tgagtgccta tctgcgctat atcgtgcaga aagcagactc
     1921 ctcctttctt tatgataagt atcagaatca gagtattgcc gcgcatgtga tgcgcgctct
     1981 cgctgctgag cagtcggaag tgtcgccaga acagcggcgc gccatctgcg aggcttttga
     2041 gtctgccaat aatacccatg gcttaaacct gactgcccat aaatacccgg gcttacgcgg
     2101 cacactacaa accgcatcca ctgactgcga cacaattgtg gaagctgcag cactattacc
     2161 cgcttttgat caggcagtgg aaggcaaccg ccaccaggat gattacggtt caggtcttgg
     2221 gatggccgaa gagaaatttc actattatct cgatctcaat gaccgctatg tctattttta
     2281 tgagccggtt aatgttgaat actttgcgat gaataactgg tccttcctgc agtcaggaag
     2341 tattggtatc gatcgcaaag atattgaaaa ggtatttacc gggcgtaccg tattgtcgag
     2401 catttaccag gatcagcgta ctaaacagaa cgtgatgagt ttgctgacgc cggtgtatgt
     2461 tgcagggcag ctaaaaggga ttgtgctgct ggatattaac aaaaacaatt tgcggaatat
     2521 cttttacact catgaccgcc ctctcctctg gcgttttctc aatgtcacgc taaccgatac
     2581 cgattcgggg cgcgacatta tcatcaacca gagcgaagat aatctgttcc agtatgtcag
     2641 ttacgtccat gacttaccgg gcggcattcg tgtctcgtta tccattgata ttctttactt
     2701 tatcacgtct tcgtggaaaa gcgttctgtt ctggattttg acggcgttaa ttttgctgaa
     2761 tatggtccgg atgcacttcc gtttatacca aaatgtgtcg cgagaaaata ttagtgatgc
     2821 gatgactgga ctgtataatc gcaaaatttt aacccctgaa ctggagcagc ggttgcagaa
     2881 actggtgcaa tccggttctt cggtgatgtt tattgcaatt gacatggaca agttaaagca
     2941 aataaatgac accctcggtc atcaggaggg ggatttagcg attacgttat tagctcaggc
     3001 gattaaacaa tcgattcgta aaagtgatta tgccatccga ctcggtggcg atgaattctg
     3061 catcattctt gtcgattcga cgccgcaaat tgcagcacaa ctgcctgaac gtatcgaaaa
     3121 acgtctgcaa catatcgcgc cgcagaaaga gatcggcttc tcttccggta tttacgcgat
     3181 gaaagaaaac gatacgttac atgatgcgta taaagcttcc gatgagcgtt tatatgtcaa
     3241 taagcagaac aaaaacagcc gttcatgata accttctgtg gttgtttgct tgtaatctca
     3301 ggagcgtgaa atgactgaaa tggctaaagg aagcgtgacg catcagcggt taatcgcttt
     3361 attatcacaa gaaggtgcta acttccgcgt cgttacccat gaagcggtag gtaaatgtga
     3421 agcggtgtca gaaattcgtg gcaccgcttt aggccaggga gctaaggcac tggtatgtaa
     3481 agtcaaaggc aatggcgtaa atcaacatgt tctggcgatc ctcgctgccg atcaacaggc
     3541 cgatctgagc caactcgcca gccatatagg cggattacgc gcttcgttgg ccagcccggc
     3601 ggaagtcgat gaactcacag gctgtgtctt cggcgc
//
LOCUS       DAEVZA010000256         3626 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEVZA010000256 DAEVZA010000000
VERSION     DAEVZA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.256"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(43..300)
                     /gene="repA"
                     /locus_tag="LAF92_005038"
     CDS             complement(43..300)
                     /gene="repA"
                     /locus_tag="LAF92_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBI9793916.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGKPLSEAERQRAASARKRSVCKEI
                     KVFVRPELKNCLTSLCAEEGVTQAELIERLIEKEACHRNMM"
     gene            705..1727
                     /locus_tag="LAF92_005039"
     CDS             705..1727
                     /locus_tag="LAF92_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBI9793917.1"
                     /translation="MTITTIGIDLAKNVFAVHGVDQNGKTVLVKSRVTRAALPGLIAS
                     LPPCVIGMEACSGAYYWARLFLQYGHEPRLMAAKFVSPYRMAGKSGKNDAADAQAICE
                     AVRRPHMRFVPVKDESQQAMQCLHRNRQGFIEEKTATYNRLRGLISEFGVIAPQSTDA
                     LRHMVSAQKSSLPLQVQQCVDDLLEHVDRIEANITEYDRILSRIAKTDHRSQRLMKLK
                     GVGPTTACALVACIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKASDSYLRT
                     LLVQGARSVLIGAEKRTDLFSRWVCSLVERRGYWRAVVAIAAKNARLCWASLHYGDDF
                     RLYSAS"
     gene            2199..2552
                     /locus_tag="LAF92_005040"
     CDS             2199..2552
                     /locus_tag="LAF92_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793918.1"
                     /translation="MSETVQIESFNAEFLTKQKLHDLIFYRKSFDITNVNDISETVKI
                     VEYVIEHQQKKLTCRVYTEYRSTAVAGSLWSPTALLGAVSAAAIGVHNLATWNPDYEI
                     GKNYVKRTISVRYKK"
     gene            complement(2965..3543)
                     /locus_tag="LAF92_005041"
     CDS             complement(2965..3543)
                     /locus_tag="LAF92_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBI9793919.1"
                     /translation="MSKLAASPIAALLIRIILRSVNQHSPLLMQLHAAGIRTGDAERI
                     LSSGECWQRQKTLLTGREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRPRSRQ
                     WWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIP
                     LLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            3576..>3626
                     /locus_tag="LAF92_005042"
     CDS             3576..>3626
                     /locus_tag="LAF92_005042"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793920.1"
                     /translation="MASVSISCPSCSATDGV"
BASE COUNT          965 a          859 c          850 g          952 t
ORIGIN      
        1 aatctagcac tgacagaaaa aactagcaag aatgtgaccc catcacatca tgttccggtg
       61 acaggcctct ttctcgataa gcctttctat caactctgcc tgagttaccc cttcctctgc
      121 acacagactg gtaaggcagt ttttcagttc tggtctgacg aaaaccttga tctctttaca
      181 tacagatcgt ttgcgcgcag aagctgctct ctgtcgttct gcttcactca aaggtttacc
      241 ctttctgtat gctcgttttg caccagatga ggaagtcact gcattttctg tctgcgacat
      301 ctcgcctcct caatactcaa acagggatcg tttcgcagag aatactacag ttttttgaaa
      361 tcagcgactt gagaattgtg acgaagatct gtgttggcgc acaaatcaac ggggattact
      421 gtcgtttaag ggctgcgttt gggaaacgta caatattgta cggttgcgtt atcgcgctcg
      481 gacgctatac ggtgtttggc agacctttag ggacatgaga taacctaaat gcgataagag
      541 aaaagcaata acttaaaggc gatagctgat cacgatgaat caatcagtta cttatcagta
      601 tcggttaaaa aatgccccta acgaatggaa gggacttccc ctggatatgc acctgattaa
      661 ctcttgtgca atcatggggt aaacatagcc tggagagatt cgttatgacc atcactacta
      721 tcggtatcga tcttgctaaa aatgtgttcg ctgttcatgg ggttgaccaa aatggcaaaa
      781 ccgttctggt gaaatccaga gtaacccgcg cagctcttcc cgggctgatt gcaagtttac
      841 cgccctgtgt tatcgggatg gaggcgtgct ccggggctta ttactgggca agattgtttc
      901 tgcaatatgg tcatgagccg cgtctgatgg ccgctaaatt tgtatctcca taccgcatgg
      961 ccggtaaatc agggaaaaat gatgcagctg atgctcaggc tatctgcgaa gcggttcgtc
     1021 gcccacatat gcggtttgtt ccagttaagg acgagagcca gcaggcgatg cagtgtctgc
     1081 atcgtaaccg acagggtttt atcgaagaga aaacagcaac atataatcgt cttcgagggt
     1141 tgatatcaga attcggcgtc attgccccac aaagtacaga tgctttgcgg cacatggttt
     1201 ctgcgcagaa gagctcttta cctcttcagg ttcagcaatg tgtagatgat ttactggaac
     1261 acgttgatcg cattgaagct aacatcactg aatatgaccg aattttgtcc cgcatagcca
     1321 aaacagatca ccgcagtcag cggctgatga agctaaaagg agttggtccc acaactgcct
     1381 gtgctctggt tgcctgtatc ggtaatgcac atgattttaa aaatggacgg caactggcgg
     1441 cctggctggg gttgactcca tcgcaataca gtagcggcgg aaagtcgaag ttaggcagga
     1501 taacaaaagc cagtgattcg tatctgcgaa cgcttctggt acagggggct cgttcagtac
     1561 tgattggcgc tgagaaaagg actgacttat tcagtcgttg ggtctgttca ctggtcgaac
     1621 gcagaggata ctggcgcgct gttgtggcca tcgccgccaa aaatgcaagg ctgtgctggg
     1681 catcattgca ttacggtgat gatttcaggc tgtattcagc aagctaaagc aggaagaaac
     1741 cgtatgacca tctgatctgc aactcgttga tgataaaggg ttagaccccg tgaggtatat
     1801 ctgattattg tacaggatat acgtatccgc ttaacgaaca agagcctcac gcgcgtcttt
     1861 catcagggtc cgaatcgata gcgattcatt atggccgttt atagtaccgc agtctctccc
     1921 ctttattttt aactgtagtg cagacacaaa ccataaatac cgacgttgac tgttcgggga
     1981 agcccttata gtacaaaaat gtccgatctc caaaccgccc ccacatacgg cagtcggttt
     2041 acgcttacag ttaagacgat caatttctta ttattgatcg tttatacaag tcaatatatt
     2101 cttttctgac atacactgcc cctgaaaaag tgcaacgttt gaccgtaagg cgcgtgccta
     2161 gggaaattca cagactccaa gaaagagagg aacaacggat gtcagaaacg gtacagattg
     2221 agtcgttcaa cgcagaattt ttgaccaaac agaaactaca cgacctgatt ttttaccgca
     2281 aaagttttga tatcacgaat gttaacgaca tcagtgaaac cgtcaaaata gtggagtatg
     2341 tgattgagca tcagcagaag aaattaacct gccgcgtata caccgaatac agaagcactg
     2401 cggtggcagg ctccctatgg tcaccaacag cacttctggg agctgtgagc gctgccgcaa
     2461 tcggtgtcca taatctggcg acatggaacc cggactatga aattggtaag aattacgtca
     2521 aaagaaccat atctgtaagg tataaaaagt aacgggttta tatctgtccg gttcagccaa
     2581 aatcctctcc cataattcct atgcgagctg aacgggggca gtctggagat cgaaaaattt
     2641 tgaacgttaa gcgcattatt taaccaatag atatgcataa cagattgatt ttgcgtcaac
     2701 gtctatccct tttaagttat cgccattgca gtattgcatt taggttatcc gattgaggtc
     2761 aaatcctctg aaaacgccgt atagcacggg agtacacccg agcgcgataa cgcaaccgta
     2821 caatattgta cgtttcccaa acgcagccct ttaatgtgat ttaaactgtg aaatagtatg
     2881 gttttcagtt atggaaacgc cgggagcggg gaaaacttgc tttttcccgt ttccggggtt
     2941 ggacaactga gcaacgcgaa ggcgtcagct acgatgttcc ggggactgct gatcagcccc
     3001 tgttgtattc agccattctc cggtcatctg cagcagtttt ctggcatcgt ggcttctgag
     3061 caacggaata ccagcctgcc tcagaacctc ttccagaaga atatcccggc gtctgcgttc
     3121 cggtcgtaaa tgactggcat catccagctc tactgctgca acaatactga acgaacgccg
     3181 ctcaacgata accacatcaa catgccactg agacaccatc ctgaataact gccaccactg
     3241 gcgtgatcgt ggccggatat tcccgttcag ctggacgata tccgcgaccc gtacctgcgg
     3301 acacagatac caccgcttca tatccacaat tctgaacagt cctttcataa aactgacttc
     3361 ccttcctgtc agcagcgtct tctgacgttg ccagcattca ccgctggaca gtattcgctc
     3421 tgcatcaccg gtccgtatac ctgccgcatg caattgcatc agcaaaggag aatgctgatt
     3481 tacacttctg aggataatcc ggataagtaa cgctgcaata ggtgatgctg ccaacttact
     3541 gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc
     3601 cctcctgttc agctactgac ggggtg
//
LOCUS       DAEVZA010000257         3596 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEVZA010000257 DAEVZA010000000
VERSION     DAEVZA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.257"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            689..919
                     /locus_tag="LAF92_005043"
     CDS             689..919
                     /locus_tag="LAF92_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793921.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            971..2332
                     /locus_tag="LAF92_005044"
     CDS             971..2332
                     /locus_tag="LAF92_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBI9793922.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPRQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2379..2942
                     /locus_tag="LAF92_005045"
     CDS             2379..2942
                     /locus_tag="LAF92_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI9793923.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVSPAPICDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(3028..3483)
                     /locus_tag="LAF92_005046"
     CDS             complement(3028..3483)
                     /locus_tag="LAF92_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793924.1"
                     /translation="MAPDRTTGRDPPQRREARFAKRRCSLHPGVFCRDAGSAGHGATR
                     VARPFRSLQTRVFASGMEARQGGDSFAGSVHDSPPRQGDARKRHKQENNKTDNNQGSR
                     KKKNSSSSSSSSSSSSSSRNNNIISNRQMTGLKRQKPPSERPRNERGGA"
BASE COUNT          798 a         1023 c         1046 g          729 t
ORIGIN      
        1 cgctacggtc cgatgcgtga aaacccctgg cttgcagcga ccgttccggt cgcgccgcct
       61 ctgtcgcgcc gtggcctgcg ctcccggcat cccggcaaaa ggccgccggg atgtaagggg
      121 caacgccgtt tcgcgcacgc gctctccgtc gttgcggcgg gcccctgctc attgcctgcc
      181 gggtgccaca cacccgtatg agtccctgtc gccgggggca tagacagcca caacctgccg
      241 cactgactgt ccagggtaaa tgaacagccc acaaaatttt ttacccaaaa accggtaaaa
      301 aattttgcgt tctgcccagg acaggtgcgt caggccgtgg cagtgatgcc ccttgcggcg
      361 aaggttctca aacgggtgaa gttcatacca cagggaatac agcagcaggt tcccgcgaaa
      421 cgtcaaaacc gcggctcccg aaactcaacg ggtgatgttc tttaaaaatc aggatttatt
      481 tcaggctccg gcaatacgca tttatttgtt ctgtcagtca tccggctggc aggtaatgag
      541 aacgcaggaa cagaaggagg aaaacaccgg agaacgtcag acgcagatat gaaaaaagca
      601 ggccggggag gacctgctta atccacaccg caaaaactgt tcgagcagtt tctgcggttc
      661 cattcaacag tcataaggag tttcatttat gacggtaagt attgtttcac cttccgcagc
      721 ggctgtaaag ccccgccgtc atccccgttt cagacgcgaa gacatccccg cgccggaaat
      781 tgaccccgtg ctgaaagcct tcgggcgaca cattgcccgg agttttcacc ggggacgtgg
      841 tgtacacatc cccgccatga aaaatacggc tttcgggcag gtgctccgca cactcgaact
      901 gaaacgcgct tttaactgat gaggtgagcc gccccgcgag gggcggcaac agacggaagg
      961 agaacatttt atgacattag ctgcaatcac catgaccgcc ccggaagccg ccagccctgt
     1021 gcagatgtac cgcgcgacct actcaccgga tgacaacaaa ctgcgcctgt atgccgcgtc
     1081 acgtcttgac ccggagacgt ataaaaaagt gcatgatgcc ggtttccgct gggcacccag
     1141 acaggcgctg tttgtcgcgc cagcctggac accgggccgg gaagacgtgc tcctctcact
     1201 tgccggagag attgaggatg aagacagcac gctcgctgaa cgtcaggaag cacgggcgga
     1261 gcggtttacc ggatacagcg gaaagcgggc cagtgaatcc gcacaggcac ttgatgaagt
     1321 ggaaagactg gccgcgatga tcccgcccgg tcagcccatt cttgtggggc atcacagcga
     1381 acgccgcgcc cgtcgtgatg cgcagcgtat tgaaaacggc atgaaacgtg ccgtgatgct
     1441 ctttgaacgt gcggaatact gggaagaacg ggcgcggtcg gcactgcttc acgcgaagta
     1501 taaagaacgc ccggacgttc gctggcgtcg tatcaaaaaa atcgaagctg atttgcgcaa
     1561 ggctgaaaag accatcgcac agtcgcagaa atatctgacg atgtggcggg ctgaatcgct
     1621 ggatctgaat atggcaaaac tcatcagcag tcatgaccat atcagcgcct gtttcccgct
     1681 ggatacgtat ccgcgcccgg cagaaaaaag ccagtatgaa gggagccggt cgttatggtc
     1741 ggccctggat gatgacatca tcaccacgga gcaggcccgc gaaattgcga tccgctgtca
     1801 tgaacggcag attcagcatc agcaacgctg ggttaaccac tatcagaacc gcctgatcta
     1861 tgagcgtgcc atgctggacg aaagcggcgg cgtggttacc cggacacagg attttgagcc
     1921 gggcggacag gttttcagcc ggggcgagtg gctgactatc atccgcgtga acaaaagcaa
     1981 cggggcggtg agttcagtca caacgccaaa ttacagtttt ctcgggtaca gcggcacgat
     2041 gaaagtgacg cccgatcgca tcacggacta caaagcacca tcggcagaag aggctgccgt
     2101 cgccagtcag gccgcgaagc gtccgccggt agtcaactat ccgggggaag gtttccggga
     2161 aatgacaaag gcgcagtggg ccgccctgcc ccgggactgt aaggccgtgc gcagtgtgga
     2221 agaagcagaa gaccacgggg cataccgcta ccgccgcaca atggacaata atttccgtct
     2281 ggtgaatgtg tatatcaccg acatgaaaat cacggaaatc ccacagaaat aaggtatatc
     2341 cccccgggaa atccccgggg gatgacggag ataatgatat gcacagtcag ttaagagaac
     2401 gtatccggct gatgcgcgca aggctggata acgccgcgcc ggttgctgaa atccgggctg
     2461 aatctcagct ttttgtgagt cctgccccga tctgtgatcg cctggtgacg ctggcggaaa
     2521 tcagcaaccg tgatcacatt ctggaaccct ctgccggcac cggggccata ctgcgggcga
     2581 ttcgggatac cgcgccggag gccatgtgtg atgcggtgga gattaacagc gggctggtcc
     2641 ggtatctgcg ggaaaatttt aacggtgtca gggtacagtg tggcgacttc atggaatggc
     2701 agccggtgca gtattacagc cggatcatta tgaatccgcc gttcagtcac gggcaggata
     2761 tccggcatat cctgcgggct ttttccctgt tgcgtccggg gggcgttctg gttgccgtct
     2821 gtctcaacgg gccacgccag caggagaagc tgttaccgtt ttctgacgtc cgcgaggagc
     2881 tgccgcgcgg cacgtttgct tataccgatg tcccgacgat gattattcgt ttgcgtgcct
     2941 gatgacacag ggctggcggg aggataaatg cccgtctgtc cgcgccggga aaaaaccgcg
     3001 cgccggtggc gctgaaaggg gccgcgctca cgcgccgccc ctttcattac gcggacgttc
     3061 tgatgggggc ttctgtcttt tcagacctgt catttgtctg ttactgatga tgttgttgtt
     3121 cctgctactg ctactgctac tgctactgct actgctactg ctactgttct tcttttttct
     3181 actgccctga ttattgtctg ttttgttgtt ctcctgctta tgccttttcc gggcgtcccc
     3241 ctggcggggc gggctgtcgt gaacggagcc agcgaagctg tctccgccct ggcgggcttc
     3301 catccctgac gcaaaaaccc gggtttgcag cgaccggaac ggtcgcgcca cccttgtcgc
     3361 gccgtggcct gcgctcccgg catcccggca aaagacgccg ggatgtaagg agcaacgccg
     3421 tttcgcaaac cgcgcttcac gtcgttgcgg cgggtccctg cccgttgttc tgtccggtgc
     3481 cattcacctg tttaagcccc tgtcgccggg ggcatagaca gccacaacct gccgcactga
     3541 ctgtccaggg taaatgaaca gcccacaaaa ttttttaccc aaaacccggt aaaaaa
//
LOCUS       DAEVZA010000258         3563 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEVZA010000258 DAEVZA010000000
VERSION     DAEVZA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.258"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..307
                     /locus_tag="LAF92_005047"
     CDS             <1..307
                     /locus_tag="LAF92_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBI9793925.1"
                     /translation="RVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGEVG
                     YTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPES"
     gene            complement(371..862)
                     /locus_tag="LAF92_005048"
     CDS             complement(371..862)
                     /locus_tag="LAF92_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBI9793926.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(964..1518)
                     /gene="ycdY"
                     /locus_tag="LAF92_005049"
     CDS             complement(964..1518)
                     /gene="ycdY"
                     /locus_tag="LAF92_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBI9793927.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(1542..2279)
                     /gene="ycdX"
                     /locus_tag="LAF92_005050"
     CDS             complement(1542..2279)
                     /gene="ycdX"
                     /locus_tag="LAF92_005050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBI9793928.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(2334..3272)
                     /gene="ghrA"
                     /locus_tag="LAF92_005051"
     CDS             complement(2334..3272)
                     /gene="ghrA"
                     /locus_tag="LAF92_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBI9793929.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT          927 a          936 c          851 g          849 t
ORIGIN      
        1 gcgcgtcgtc aatgtgagta ccggtgagat cctctcctcg gtgaacacca gtaagacgat
       61 actttcctat gaagttcagg ccggggtttt ccgctttatt gactaccagc gcttgctgga
      121 aggggaagtg ggttacacct caaacgaacc cgttatgctg tgtctgatgt cggctatcga
      181 aacaggggtc attttcctga ttaatgatgg tatcgaccgt ggtctgtggg atttgcagaa
      241 caaagcagaa cggcagaacg acattctggt gaaataccgc catatgtcgg ttccaccgga
      301 atcctgacag ataaaaaagc cggaattatc atccggcatt ttactgttta agcgaaagcg
      361 tgagtgcccc tcacgcttta tttttgattt cctgctcacg gtgagccgtc tttgcactac
      421 gcgccgccag ccacaggccg ctgtttttca ttgcgtaacc aaataccagc ccaagcgcca
      481 gcgaaggtaa caccagtttc cagtcaccct gcccggcaaa cgtcgcgcat gcgcctataa
      541 acgtccccgg aacaaatgaa agcagcagct gtttggcctg aatacacatc agaaacgcga
      601 caataccggt tatgacataa ccgagaattt ccagatgtgg tgccagtgca ctaccgtaaa
      661 taatgaccat cgcccacacc acgccactaa gcagggttgc agcggagatc gccagccctt
      721 tcagcccacc ttgcgggcag gcaaagtagg ccgtacagcc caggaagccc gcccagctta
      781 gtaagcccag ggaaacagcc acccatcccc agataccgga gagaatgcct gttgtgattg
      841 caatagagag aagtatgttc atgccgcgca tgatagcaga aaacgcggcc atgaatgaga
      901 tcgagcacac attttatgca acaacaaatt atctgttgca gttatgagtg acgatgatca
      961 catttactct tcagaatctt cttccagctc gtcccacatt gcactaatgg catcacgggt
     1021 tagcggtgcc atggtgcgcc agaaaggcgt ggttgcatgg gcctccactt tgccgaggaa
     1081 cgcaccacac cagggtaaca gatactcact gaacagtgtt tccagtgctt cgctctcatc
     1141 ttccgttgac tgatcttcca gccaggaagc cgcaagcagc aatgtgccga tgtgatcggc
     1201 tggcgtatcc gccagtggca tccctcgttc ggaaagaaaa gcgcgcactt ccgcttccgt
     1261 tgcgtcctca acccatgcgc tacgatatgg cggcacggca cattcatcgc cgataaacaa
     1321 cgcattgtaa tcggcagaga cttgcgccat atcacagctt ttctgtaaac gcgtcagtaa
     1381 ctcatcctgc tccagtggcc agttcgcagc cagtttcccc tcacgaatca gggtaaacag
     1441 cggcaccagt aaaggatctt gcggttggcg gtaatagagc gaacccagca cacgacagag
     1501 gatagaaaac tcgttcatta atatatttcc attactagta attaaaggtc tgcaaattcc
     1561 gcaatcggtg ccataccgcg agattcaagg aagttcagta agcggcgcgg cgaaacattc
     1621 aaaatgcgct ctggcggaaa atctaccgcg tcgaggattt taagacactc ttcaaattcc
     1681 cccatggtaa acgcggtgtg agaatccgag cctaatgcca cccaacctcc agcatcacgt
     1741 accgctgcgg ctaccgcacg acagttgtct tcactgccct tacgtgagtg taaaaatgaa
     1801 gaattattga tttccagcgc cacctgatgt ttcgcggctg cttcagcaac ggctttcaca
     1861 tctatttcat atttgggatt tccgggatgg cttattatat gcacattgcc gctggcgata
     1921 gtggcgatca tcgcttgtgt attggtcgct ttgtcatgtg gtgcaaaaac cggctcatga
     1981 aaaccggcaa taatcagatc cagcgagtca aacattttac cgctgcaatc aatttcacca
     2041 tcaacatttt taatgttagc ttcgatgccg cgcaggatcc ctaccccatc aaccactcgc
     2101 ggccagatac gcatgttaat gaagtgccag tgatgcggag catcttccat atccgggcca
     2161 tgatcggtga tcgcaaaaag tttaatgccc ttttgtttgg cctgggcaat gtaatcactc
     2221 aatgtgctat atgcatgtgt gctggcaacg gtatgcatat gaaggtcgac gggatacata
     2281 actctctcct gtattcaatt cccgccaagg atagcaggaa tcctgatgct ttattagtag
     2341 ccgcgtgcgc ggtcgacttg cccgcagacc ctctcccctt tttcgagctg ggcaatggtg
     2401 cgagaaatgt actccacagc ttcagcggga cgggtaatcg cggcgacatg tggtgttatc
     2461 gtcacgcgtg gatgttgcca gagcggactt tcaggcggta agggttcacg attaaaaaca
     2521 tccaacattg cgcctttaac tttgccgcta tccagcgccg cgagcaggtc atcttccaca
     2581 acatgaacac cacgcgccag gttgaggaga tacgcgccat ccggtaattt ttcgagtaat
     2641 tgttgattaa taatgccgac ggtttcaggg gtattcggta acaaattaat caatacccga
     2701 cattggctca gaaatgcaga cagttcttcc cgtccggcaa agctttgcac gccaggccac
     2761 gatttacggg ttcgactcca gcaacgcagc ggaaagcgcc aggtttgcag actctgagca
     2821 actttactgc ccagtacgcc tgcgcccaaa atgccgatgg taaaatcttc ccgatgatat
     2881 tcaggcagcg gttgccaatg cgaactattt tgctggatgc gataatcgtc aaaacgtcga
     2941 aaccaatgca gcacctgact gacagcatat tcctgcattt gctcgcccat accggtatct
     3001 tccaggcgaa aaagtggaac agaagggttc agcatttcag ggtgtgcctg tagcttgctc
     3061 aaaatagaat caacaccggc cccgagtgcg aacaccgctt taagatcgcg ccctgccagc
     3121 atttcaacag gaggatgcca gactaaagca taatcagcag agtcattatc tccgcttttc
     3181 catgctctga ctcttgcctg aggaatagct ttgcgcagtg cctcaatcca ccattgggta
     3241 tcgaacgttg ggtgataaaa gatgatatcc atactgactc ccgaaaagcg ttgtgcgaaa
     3301 tttattcgca cttatcgtta tgatctacaa aggccaccag cataacaaat ccgtggtcgg
     3361 tggcaaaaaa agcagatttc gcttattaaa accacacatt gattgaagtt tgaataaacg
     3421 cgcgattttt tcaaaaagtt tgttgacctc aggtcatgat ttccctaaat tagcgcccgt
     3481 tccagcaaga caggaacgac aatttggtga ggtgtccgag tggctgaagg agcacgcctg
     3541 gaaagtgtgt atacggcaac gta
//
LOCUS       DAEVZA010000259         3439 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEVZA010000259 DAEVZA010000000
VERSION     DAEVZA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.259"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1878)
                     /gene="maeB"
                     /locus_tag="LAF92_005052"
     CDS             complement(<1..1878)
                     /gene="maeB"
                     /locus_tag="LAF92_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBI9793930.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IE"
     gene            2167..3117
                     /gene="tal"
                     /locus_tag="LAF92_005053"
     CDS             2167..3117
                     /gene="tal"
                     /locus_tag="LAF92_005053"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBI9793931.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3137..>3439
                     /locus_tag="LAF92_005054"
     CDS             3137..>3439
                     /locus_tag="LAF92_005054"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBI9793932.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPE"
BASE COUNT          817 a          938 c          845 g          839 t
ORIGIN      
        1 ctcaatacca aaacgacgga cagtttctgc cgccatcaag gtgatctccg ccagctcttc
       61 tgcgtctggt tcgtcattga cgtaggtatc ggcaataaag gtgttaccac tcggcagcag
      121 cagcgcgttc attgcacctg cggtgtgaac gccatcgcga taaccaaaga catttttcac
      181 cacgctaaaa tgttcatgat aatcgcccac cgtaccgcag atcattgcat cggcttcccc
      241 acgctgaacc atgatcgcgc cgatcactgt cgggttactg atcagcgccc gctgcgcctg
      301 ttcctgagtg acaccgcgac gcttcatgat ctggaagtat tcggtccagt actctttaaa
      361 gcgcggatcg gattcgttat tgacgatctc aaaatcaacg cccgctttga tctgcaagcc
      421 cagtttctga atgcgcattt cgatcacgtt cggacgaccg ataaggatcg gtttcgccag
      481 tcccagcgtt accagttcct gagtggcatg cagaacgcgc gcctcttccc cttccggcag
      541 aactacgcgc ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa acaggttggt
      601 tttgtaaacg aactcagtca gcttgtcgat gtagacgtcg aaatcagcaa tcggacgcgt
      661 cgccacgccc gactccatcg ccgctttcgc caccgcagga gcgatcttaa cgatcaagcg
      721 cggatcaaac ggttttggaa tgatgtattc cggaccaaag ctcagatcct gatcgccata
      781 cgctgaagcc accacttcgc tctgttcggc gtgggccagt tctgcaatcg cgcgtaccgc
      841 cgccagtttc atctcttcgt tgatggcggt tgcgccaacg tccagcgcgc cacggaagat
      901 gaacgggaag caaaggacgt tgttgacctg gtttggatag tcagaacgac cggtgcagat
      961 gatggcatcc ggacgcactt ctttcgccag cggcggcaga atttccggtt ccgggttcgc
     1021 cagcgccagg atcattggcg cacgagccat tttcttcacc atttcctggg tcagcacttt
     1081 cgggccggaa catccgagga aaatatccgc gccttcaatc acatcatcga gggtacgttt
     1141 gccgtcatcc accaccgcat atgcggcttt ggtttccgcc atgtttggct cacggccctg
     1201 atagataacg ccttttgaat cgcaaaccac gatgttatgt ttttgcagac ccagcgctac
     1261 cagcaggttc atacaggcga ttgctgcggc acccgcgccg gaaaccacca tccgcacgtc
     1321 ggagatgttt ttctccacca cgcgcaagcc gttgaggatg gcggcagtgc tgatgattgc
     1381 cgtgccgtgc tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca gtttctgttc
     1441 aatatagaaa cattctggcg ctttaatgtc ttcgaggttg attccgccga aggtcggttc
     1501 gagcgcagcg acaacttcaa taaatttgtc cgggtcgagt tcatcaactt caatgtcaaa
     1561 tacatcaatc ccggcgaatt tcttaaacag aacgcccttg ccttccatca ccggtttgcc
     1621 agccagcgcg ccaatgttgc ctaaccccag caccgccgta ccgttagaga tcaccgccac
     1681 caggttaccg cgcgcggtat atttgtaggc ttttaacggg tctttttcga tttcaagaca
     1741 aggtgcggca acgcctggtg agtaggccag cgccagatcg cgctgcgttg ccagaggctt
     1801 ggttggagaa acctggattt tccctggaac tggaaattca tggaaatcaa gtgcactttg
     1861 ttttaactgg tcatccattt ggttgttcct ttcacgtaac gttcacaaat aaagtgtgtg
     1921 ggcaacagcc cctgcccaca acgtggcgca cattattacc ctgccggagt ctacagactt
     1981 tgagcaagtc caaactctca ccattaatat aatgttttgg taataatcct ataacactga
     2041 tgttacctgc ttaatccagc aataccatgc ctgtctgcta tgcttttttg atgcgtttag
     2101 cgaaatttct cagaagtgtg aattaacgca ctcatctaac actttacttt tcaaggagta
     2161 tttcctatga acgagttaga cggcatcaaa cagttcacca ctgtcgtggc agacagcggc
     2221 gatattgagt ccattcgcca ttatcatccc caggatgcca ccaccaatcc ttcgctgtta
     2281 ctcaaggctg ccggattatc acaatatgag catttaatag acgatgctat cgcctggggt
     2341 aaaaaaaatg gcaagaccca ggaacaacag gtggtcgcag cgtgtgacaa actggcggtc
     2401 aatttcggtg ctgaaatcct caaaatcgta cccggtcgcg tgtcaacaga agttgatgca
     2461 cgcctctctt ttgataaaga aaagagtatt gagaaggcgc gccatctggt ggacttgtat
     2521 cagcaacaag gcgttgagaa atcacgcatt ttgatcaaac tggcttcgac ctgggaagga
     2581 attcgcgcgg ccgaagagct ggaaaaagaa ggtattaact gcaacctgac gctgctgttt
     2641 tcttttgccc aggcgcgggc ctgtgcagaa gcgggcgttt ttctgatttc gccgtttgtc
     2701 gggcgtattt atgactggta tcaggcacgc aagccgatgg acccgtatgt ggtggaagaa
     2761 gatccgggcg ttaaatcggt gcgcaatatc tacgactact ataagcaaca ccactatgaa
     2821 accattgtga tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt aaccggctgc
     2881 gatcgactga ctatcgcacc gaatttgctg aaggagctgc aggaaaaagt ttcgccagtg
     2941 gtacgtaaat taatcccacc ttctcagacg ttcccacgcc cagctcccat gagcgaagcg
     3001 gagttccgtt gggagcacaa tcaggatgcg atggcggtag aaaaactgtc tgaaggcatt
     3061 cgtctgttcg ctgttgatca acgcaaactg gaagatcttc ttgccgccaa actataaacc
     3121 agccacggag tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg cactcagtat
     3181 ggatgcggta caaaaagcca attctggtca tcccggcgcg ccgatgggca tggctgatat
     3241 tgccgaagtg ctgtggaacg attttcttaa acataaccct accgacccaa cctggtatga
     3301 tcgcgaccgc tttattcttt ccaacggtca cgcgtcgatg ctgctctaca gtttgctaca
     3361 tctgaccggt tacgacctgc cgctggaaga actgaagaac ttccgtcagt tgcattcgaa
     3421 aaccccaggc cacccggag
//
LOCUS       DAEVZA010000260         3420 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEVZA010000260 DAEVZA010000000
VERSION     DAEVZA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.260"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1356)
                     /gene="cheA"
                     /locus_tag="LAF92_005055"
     CDS             complement(<1..1356)
                     /gene="cheA"
                     /locus_tag="LAF92_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBI9793933.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AM"
     gene            complement(1361..2287)
                     /gene="motB"
                     /locus_tag="LAF92_005056"
     CDS             complement(1361..2287)
                     /gene="motB"
                     /locus_tag="LAF92_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBI9793934.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(2284..3171)
                     /gene="motA"
                     /locus_tag="LAF92_005057"
     CDS             complement(2284..3171)
                     /gene="motA"
                     /locus_tag="LAF92_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBI9793935.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(3298..>3420)
                     /locus_tag="LAF92_005058"
     CDS             complement(3298..>3420)
                     /locus_tag="LAF92_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="With FlhD is involved in the activation of class 2
                     flagellar genes and as well as a number of other genetic
                     systems; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FlhC"
                     /protein_id="HBI9793936.1"
                     /translation="GSFACSLCQPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
BASE COUNT          771 a          939 c          844 g          866 t
ORIGIN      
        1 catcgcgact tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt
       61 tgccaaaatt cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt
      121 gccgccctga tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag
      181 ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg
      241 gtcgataatg cgttctatca ggctcttgtc gagctcggtg gaactgccca ccagcgtcag
      301 ttctacctgc ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa
      361 gacatattcc atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt
      421 acgttgtaac tgccccatgc tggtaatcaa atcaccatga ttaaccgggt ccagttcgct
      481 ggaacgctgg gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg
      541 atcaaccttt tctaccgcta cacggatgct ggtggattca ctgctgcgcg tcgttttttc
      601 ccgctccacg cggccggttg gcgcttgttc ggctgccagt ttaagcacag gtggggtagc
      661 tatttttggc gagacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca
      721 gagtaccgct gtgatgtcat cttcggcgat gtcgcccggt aatattgccg agagcgaatc
      781 cgcccctttc accacgtcag ttaacgttgt cagatgcccc agctcttctt ccagcaggtc
      841 gacttcgccg gccttcaggc gcgaaaggat aattcgtcgc ggcaactgac tgcgactctg
      901 ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc
      961 acctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcga agctggcggc
     1021 atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc
     1081 caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag
     1141 caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc
     1201 tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc
     1261 cggctgcaaa accagcagat gctgctccat atcagccaac agttcgtccg cttcatcaaa
     1321 aaatgtctga taaaaatcgc ttatatccat gctcacgctg tcacctcggt tcggctgatg
     1381 gcattgtggg aacactgacc tgtggtgcaa cctcaggttt ttccagggcg cttactggct
     1441 cattctggct ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca
     1501 gcaggctgat gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg
     1561 ccgccatgcc gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc
     1621 ggcgggatgc attggcccga tcggcagaaa gctcccagtt gctgtaacct ttctcaccac
     1681 tggcgtatgg gaaatcatcg gtatgacccg aaaggctaat acggttggga ataccattca
     1741 gaacgggcgc gatggcgcgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg
     1801 ttttaaacat cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca
     1861 gatcgatttt gagatgtgga cgtaacgccc gcagtttcgg atcggactct atcaactggt
     1921 cgagatcacc ccgcaatttc cgcaatcgac tttgctccat gcgttttttc aactcttcga
     1981 tgttcggctg cttattcact tccccctggc tttgggtgta atcatcacca ccgccgggaa
     2041 ttgggctttc actattagaa atgcgatcgc cgcccgtaac cgcagtcgcc agtggagtcc
     2101 ggaagtactc cgcaatctga atcagctctt ttgggctgga gatggagatc agccacatca
     2161 ccagaaaaaa ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat
     2221 gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat
     2281 tcttcatgct tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc
     2341 ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac
     2401 ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat
     2461 cattttgctg gtttcggcgc ttttctgacg taaaacactc gctaatgggg aaataaatcc
     2521 gtaagccaat aaaatgccga ggaaagtccc caccatcgca tgtgcgataa gcgcacccag
     2581 ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg acccccatta cagccgcgac
     2641 aataccaaac gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc
     2701 ctcgctttcg tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat
     2761 atgaccgctg ataatcagac gcagataatc gacgataaaa tcaagcatga cgctatccgc
     2821 gaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc
     2881 cagcgaaaac atccccatct gccgcgattt cgccatcaac cgataaagca gagccagcag
     2941 atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgccttcag
     3001 cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc
     3061 aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata
     3121 accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc
     3181 cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggca
     3241 aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt gcgcctcacc gtatcagtta
     3301 aacagcctgt actctctgtt catccagcag ttgtgggata atatcggcag gattctggga
     3361 aagtttacgt ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc
//
LOCUS       DAEVZA010000261         3382 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEVZA010000261 DAEVZA010000000
VERSION     DAEVZA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.261"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..435)
                     /locus_tag="LAF92_005059"
     CDS             complement(<1..435)
                     /locus_tag="LAF92_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBI9793937.1"
                     /translation="MNIVPLNYKGEIVSFNTDGWINVTGVAERFGKRIDNWMRLAETL
                     EYVRALDEALTGKESQILHPSQSRYVKTSKARKDRGGGTWLHPKLSVAFARWCDARFA
                     VWCDLHIDSLLRGELTEQQKYEQACRIRDDRKSKASNGAREMA"
     gene            complement(640..1077)
                     /locus_tag="LAF92_005060"
     CDS             complement(640..1077)
                     /locus_tag="LAF92_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBI9793938.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRDDVSAGRKRLQVAASCAKSKTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            complement(1074..1550)
                     /locus_tag="LAF92_005061"
     CDS             complement(1074..1550)
                     /locus_tag="LAF92_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBI9793939.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(1534..1857)
                     /locus_tag="LAF92_005062"
     CDS             complement(1534..1857)
                     /locus_tag="LAF92_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HBI9793940.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(2454..2972)
                     /locus_tag="LAF92_005063"
     CDS             complement(2454..2972)
                     /locus_tag="LAF92_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBI9793941.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKRAIASYYH
                     ATAKPRKMCGRGGEGWRKPSLATCRNEIDDILKASLFVLYQPMQNAFKMRKRVEKVKH
                     VAVKNLDMQLAI"
     gene            complement(2963..>3382)
                     /locus_tag="LAF92_005064"
     CDS             complement(2963..>3382)
                     /locus_tag="LAF92_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBI9793942.1"
                     /translation="SERGNVNHWLLNGGGWFFNLDYDKEILAKALAHKADELPLIIEL
                     VSKDKKYVICHADYPCDEYEFGKPVDHQQVIWNRERISNSQDGIVKEIKGADTFIFGH
                     TPAVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGAWA"
BASE COUNT          816 a          876 c          761 g          929 t
ORIGIN      
        1 agccatctct cttgccccat tgctggcttt tgatttccgg tcatcgcgaa tgcgacatgc
       61 ttgctcatat ttctgctgct cagtcagttc accgcgaagc agactatcaa tgtgcaggtc
      121 gcaccacaca gcaaaacgag catcacacca acgggcaaat gcaactgaaa gttttggatg
      181 tagccacgta ccaccacccc tgtcctttcg tgccttgctg gtttttacat acctcgattg
      241 tgagggatgt aaaatttgag attctttccc agtcaacgct tcgtctaaag cacgaacgta
      301 ttcaagcgtt tctgccaaac gcatccagtt atcaatgcgt ttcccaaatc tctcagcaac
      361 acctgtgacg ttgatccaac catcagtgtt gaaactgaca atttcacctt tgtaattaag
      421 tggcacgata ttcataacgt ttacctacca tttgaaatga acctttgccg cacaggaaac
      481 cagcccaccg aggctcgcca gcactaactg gtatcctcaa aggcccattc caaaggggca
      541 ggttcggtgt aaaaaaaaca tgcgttgcgg tacgcattta ttgcaaaaaa gccccgcatc
      601 gcgaggctca ttaaatggac tttgtgattt gcaaaaaaat tatttcagac attgcgtcct
      661 gatgtactcc tgcaggtagt taacctgcgc ggttatcctg tcgattccac ttcggagacg
      721 gtaataattg agttcagcat ctgctgtaag tcttgggctt tctccatcgc ccatgctgct
      781 ggctccggtc tttgactttg cacaggaggc ggcgacttgc agccgcttac gcccagcaga
      841 aacatcatca cggagacttt cgatagtcgc gttagcatca gcaagctcct ttgtatatct
      901 ggcatcgagt tctgctacgt cacgttgacg cttctgcatg tcagcgatga tggatgtggc
      961 tttgtcgcgc tgctctttgt aggcgattgc gttatcacgg taatgattaa cagcccatga
     1021 caggcagacg atgatgcaga taaccagagc ggagataatc gcggttactc tgctcatacc
     1081 tcaatctctc ttaccgttcc gccagcttct ttgaattttg caatcaggct gtcagcctta
     1141 tgctcgaact ggccataacc agccccgggc aacgaagccc agatattgct gcaacggtcg
     1201 attgcctgac ggatatcacc gcgatcaatc atcggcaaag cgccacgctc tttaatctgt
     1261 tgcaatgcca cagcgtcctg gcttttcgga gagaagtctt tcaggccaag ctgcttacgg
     1321 tatgcatccc accaacgtga aagaagctgg taacgtccgg cggctgttga tttgagtttg
     1381 gggtttagcg tgacaagttt gcgagggtga tcggagtaat cagtgaatag ctctccgcca
     1441 acaatgacgt cataaccatg atttctggtt ttctgccgtc cgttatcagt tccctctgac
     1501 cacgccagca tatcgaggaa cgccttacgt tgattattga tttccaccat cttctactcc
     1561 ggctttttta gcagcgaagc gtttgataag cgaaccaatc gagtcagtac cgatgtagcc
     1621 gatgaacacg ctcgttatat aagcgagatt gctacttagt ccggcgaagt cgagaaggtc
     1681 acgaatgaac caggcgataa tggcgcacat cgttgcgtcg attactgttt ttgtaaacgc
     1741 accgccatta tatctgccgc gaaggtacgc cattgcaaac gcaaggattg ccccgatgcc
     1801 ttgttccttt gccgcgagaa tggcggctaa caggtcatgt ttttctggca tcttcatgtc
     1861 ttacccccaa taaggggatt tgctctattt aattaggaat aaggtcgatt actgatagaa
     1921 caaatccagg ctactgtgtt tagtaatcag atttgttcgt gacagatatg cacgggcaaa
     1981 acggcaggag gttgttagcg caacctcatg ccgcccgctt tcacgaagat catgtgtaga
     2041 aggccgcagc gtaactatca ctgatgaatt caggatagcc agtggctacg gctcagtttg
     2101 ggttgtggcg gtcggtgctg aactccgact taatgacgat aggcgtgtac cgacgcctcg
     2161 ttttacttcc tccgctttca cggcttcacc ctagaccagc tttacgaaat cctcgtaaac
     2221 ctaaccgcgg cagatatgac cggcacggtg tgccatatca cggaccggcg ggtgtctcgt
     2281 tcacctgatt aacgcatcag cctgcgtatt caccacaacg ataagagcac tgcgcggcac
     2341 ctttcaccaa ttccgcgagg tctgcgggtt caatgctctt acctgttgtg caaacaaaaa
     2401 agccaccatt gcaacttaag agtcactaac ggcagcttac cctctaatta tggctaaatg
     2461 gctaattgca tgtcaaggtt tttaacagca acatgcttaa ctttctcaac acgtttacgc
     2521 attttgaaag cattttgcat tggttggtac aaaacaaata atgacgcttt caggatgtcg
     2581 tcaatttcgt ttctacaggt tgccagtgaa ggttttctcc atccctcgcc accacgtcca
     2641 cacatcttgc gtggctttgc agtcgcgtga tagtaggatg caattgctcg cttagatgaa
     2701 ccatgagcgt agtagctgag gaggattcca aaggctttct tgtcaatgta catgacggaa
     2761 tcgacgacct gagaaatcaa cattccatca tcatcattac acattggcct tgtcataact
     2821 cttcccggct ctacgctctc catgaacttc gctattacgc tgctcatgcg cttttccaga
     2881 cgacctgaat aaacccatgc gccccacagt tcaagccagc cattcagcca ctcatgctgt
     2941 tctttggtga ggtttagttc tcttatgccc acgcgccttc tccctgtacc tgaatcaatg
     3001 tgaggtttcc gcagaacact gcgccggtat cgatatacat ctggttggca aacttgagtg
     3061 gtttcactgc tggcgtatga ccaaagatga acgtgtccgc gcctttgatt tctttcacga
     3121 tcccgtcttg tgagttgctg attcgttcgc ggttccagat tacctgctga tgatcaactg
     3181 gctttccaaa ctcgtattcg tcacaaggat aatcggcgtg gcagatgaca tattttttat
     3241 ctttactcac cagttcgatg attaacggaa gttcatctgc tttatgggca agagctttag
     3301 ccagaatttc tttgtcgtaa tcgagattaa agaaccagcc accgccatta agcagccagt
     3361 gattgacgtt tccgcgctct ga
//
LOCUS       DAEVZA010000262         3345 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEVZA010000262 DAEVZA010000000
VERSION     DAEVZA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.262"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(822..2270)
                     /gene="cusS"
                     /locus_tag="LAF92_005065"
     CDS             complement(822..2270)
                     /gene="cusS"
                     /locus_tag="LAF92_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBI9793943.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFAHDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVG
                     GKPIYTLYIALSIDFHLHYINDLMNKLVMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQQELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            complement(2260..2943)
                     /gene="cusR"
                     /locus_tag="LAF92_005066"
     CDS             complement(2260..2943)
                     /gene="cusR"
                     /locus_tag="LAF92_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBI9793944.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3100..>3345
                     /locus_tag="LAF92_005067"
     CDS             3100..>3345
                     /locus_tag="LAF92_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="with CusA, CusB and CusF is part of a cation efflux
                     system that mediates resistance to copper and silver;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBI9793945.1"
                     /translation="MSSCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAML"
BASE COUNT          905 a          816 c          785 g          839 t
ORIGIN      
        1 actggttcag atgaaaggaa accaccgcaa aagcatccgg cggcaggccg tccacttcca
       61 gtgtgaaacg taatccggtt gacatagcaa cctccagtaa aatgaataat gatccgtacc
      121 gtacaactgc ggataatgtg ataagcggga cgacttcaga cgttattagg gaaggaacag
      181 aaaatgctat ggttatgtga gaattttgtt taaatattac tttacagaaa cgggtgtctg
      241 ttcagtcaaa aaaataatca caagctcaac ctcttgtttc ttggcgttat tcaggacagg
      301 tacgcatatc acaacgagta acaaaaacgc aaagttaaac caatattcat aatatatttt
      361 tattcacagc aataaataat ccaattaaca ttattaaaat gaatgattgc ataagaaata
      421 tatgctaccg ctccaccctg ataacatgat ctgcacattg cacatcttaa cgttgttaaa
      481 tactggctca atagtggata ttttaaatta ggaatttatc aaattcactt gataaataga
      541 ttgagtgaat aatataacaa ttaatgatga ttaatcgcga ttaaattatt cattgcttta
      601 tatgatagat ggcttgttta taacgatgaa aatttagaaa gggcattgtg atctacaatt
      661 ggaagaaaca cagatttaac taagtttcat atacaaaaat ctcgtgaaac tttgaaaaat
      721 aaaagccttc gatatttcat atgtttaata agtttttctc taatttgttg atcaacaata
      781 ccaggaggtg atgcaccacg ccctgaattt gaacttaata atcactctct ctccggcagc
      841 ataatcacaa accttgtccc ccgcgcatct gacgttaccg caaccgtgcc tttatgcgcg
      901 acaacaatcg atttcactat cgccagccca ataccgctac cttcaccttt tcgctggcgg
      961 gaagggtcaa cgcgatagaa acggtcaaac aatcgcggta agtgctcggg cgcaatgggc
     1021 gtaccggggt tttcgacgga aacttgcacc tgatgattga ccgtctggca gcgcactaca
     1081 atagcctcac tgggtggcgt ataacgcagg gcattagaaa gcaggttgct taacgcccga
     1141 cgcagcatca gcggatcgcc cgcaacctga catttgtcgc caacaaaccg caactccacg
     1201 ccgcgatctt ccgctaacgc ctcgaaaaaa tcgaacactt tgccgacttc atccgccagg
     1261 ttgagcattt tcttttcggg gattagctgg ttgttatcgg cctgagcgag aaacagcata
     1321 tcgctgacca ttttcgccat tcgcgtcagc tcttcgagat tagagtagag cacatcttcc
     1381 agctcctgct ggctgcgcga ctggctgagg gcgatttccg tttgcgtaat gagattggta
     1441 atgggtgtgc gaatttcgtg agcgatatcc gctgagaaat tggactggcg ggtaaagaca
     1501 tcctcaatac gctcgatcat atggttgaac gacagtacca gctgttccag ctcaatgggc
     1561 acggtctgcg ggtcaaggcg aacgtcgaga tctttcgagg tgatattctg gatttgacgg
     1621 ctgacgctgc ggatcggtgc gtgaccttta tgtaccgcca acagcacgat aaagacgatc
     1681 aggatgctga ttaccgatgc ggtcattaca agtttattca tcaaatcatt tatgtaatga
     1741 agatgaaaat cgatcgaaag cgcgatgtag agcgtataaa tcggtttgcc gcccaccaac
     1801 gggccaaccg gcaagttaat catccgccag ttgctgtgtt ccatatgccc gtgaccgtgc
     1861 cctggcatca taatcgtcgg gccggaaagg agatacacct cgccgccccg cgcgtcttta
     1921 tcgggtatgg catcatgtgc aaactcgcgg atatcgggcg caccggggga gtgatacacc
     1981 gttttaccgt ggctatctgc cagggagatc aacacattgg aataaccact gacgatatct
     2041 tccagcgtca ttaagcgtcg ggcttgcgtt tcgtcagggt gatttagtac ccgttcaagg
     2101 gtggcgctaa tctcttttaa atcattaata tcctgctcgg caaaatgcac ttttactgag
     2161 tggatcatga tccatgcaaa ggcgaaaaac gccgcgatgg tggccaggct gataaaaaag
     2221 gtcaggcggg ttgccagcga aaacgggcgc tgaaatggct tactgaccat ccggcacctc
     2281 aagcatgtaa cccacgccgc gcacggtttg aatcagcttt ggctcaaagt cgttgtcgat
     2341 tttgccgcgt agccgcttca ccgccacatc aatagcgttg gtatcgctgt caaaattcat
     2401 gtcccatacc tgcgaggcga taagcgagcg gggcagcact tcaccctgat ggcggagaaa
     2461 aaactccagc agagtaaatt ctttactggt cagagtgatg cgcgtgccgc tgcgggtgac
     2521 tttgcggctg acgagatcga ccatcagatc ggcaacctga aactgacttt cgataatcac
     2581 cgccgccccg cgccgcagga gagtgcgcac ccgcgccagc agttcagcaa aagcgaacgg
     2641 tttcaccagg tagtcatccg cccccaactc cagccccttg acgcgatgtt caatggtgcc
     2701 aagcgcggta agcaacagaa tcggcatccc tttattagcg gaacgtaaca tgcgcacgat
     2761 atcccagccg ttcacgtccg gcagcataat atcgaggatt atcaggtcat aatcaccggt
     2821 catcgccaga tggtagccat tcagcccgtt gtcggccaaa tcgaccacaa aaccggcttc
     2881 ggttaaccct ttggtcaagt attctccggt tttcttttca tcttcgacaa tcaacagttt
     2941 catatttcct ccgcatgttg cccgggcaat tctagagtag cgggatcaga tggcaatcgc
     3001 ttattggcaa aatgacaatt ttgtcatttt cctgtcaccg gaaaatcaga gcctggcgag
     3061 taaagttggc ggcataaaat catcagaaat tatgagccta tgtcttcttg taaacttctg
     3121 ccattttgtg tggcccttgc gctaaccggt tgttcactgg caccggatta tcagcgtccg
     3181 gcaatgcccg tgccgcagca gttctcactc agccagaacg gcctcgttaa cgcagcagat
     3241 aactaccaga acgcgggctg gcgcaccttt tttgttgata atcaggtgaa gacgctgatt
     3301 agcgaggcgc tggtgaataa ccgggatttg cgcatggcga tgctg
//
LOCUS       DAEVZA010000263         3340 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEVZA010000263 DAEVZA010000000
VERSION     DAEVZA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.263"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            308..>3340
                     /gene="yeeJ"
                     /locus_tag="LAF92_005068"
     CDS             308..>3340
                     /gene="yeeJ"
                     /locus_tag="LAF92_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
                     /protein_id="HBI9793946.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYKTPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSVTMTATVRDAKGNLLNDVMVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQYMTATLQDKNGNPLK
                     DKEITFSVPNDVASKFSISNGGKGMTDSNGVAIASLTGT"
BASE COUNT          935 a          835 c          809 g          761 t
ORIGIN      
        1 aaaaattcgc ttttttagcg caatgtcact gaccttagtt gaacattgtt ttttaacgga
       61 tagcgggttt ttaacatctt aagcgccctc gacctttatg gttgagggcg ttttgctatg
      121 aacgccatca ccattttccc ctcgattata aaacttgagt tattcagtag tctcccctct
      181 tgcaactcac acccaaaact gcctaacgaa aagttattaa ttttcaatca tattgctatc
      241 agtatttaca ttttttcgct gtgctagaaa gggcgcattt atgttagctc gttcagggaa
      301 ggtaagcatg gctacgaaga agagaagtgg agaagaaata aatgaccgac aaatattatg
      361 cgggatggga attaaactac gccgcttaac tgcgggtatc tgtctgataa ctcaacttgc
      421 gttccctatg gctgcggcag cacaaggtgt ggtaaacgcc gcaacccaac aaccagttcc
      481 tgcacaaatt gccattgcaa atgccaatac ggtgccctac acccttggag cgttggaatc
      541 ggcccaaagc gttgccgaac gtttcggtat ttcggtggct gagttacgca aactcaacca
      601 gtttcgtacg tttgctcgag gttttgataa tgtccgccag ggtgatgaac tggatgtccc
      661 ggcacaagtt agtgaaaaaa aattaacccc gccgccgggt aatagcagtg acaacctcga
      721 gcaacagata gccagtactt cacagcaaat cgggtctctg ctcgccgaag atatgaacag
      781 cgagcaagcg gcaaatatgg cgcgtggatg ggcctcttct caggcttcag gcgcaatgac
      841 agactggtta agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg aagattttag
      901 cctgaagaac tcccagttcg attttctcca tccgtggtat aaaacgcctg ataatctctt
      961 tttcagtcag catactctcc atcgtactga cgagcgtacg cagattaaca acggcttagg
     1021 ttggcgtcat ttcactccca catggatgtc gggcatcaac ttctttttcg accacgatct
     1081 tagccgttac cactcccgcg ccggcattgg cgcggagtac tggcgcgact atctaaaatt
     1141 aagcagtaac ggctatttgc gactgaccaa ctggcgcagc gcacctgaac tggacaacga
     1201 ttatgaagca cgcccggcca atggctggga tgtacgcgca gaaagctggc tacccgcctg
     1261 gccgcacctt ggcggtaaac tggtctatga acagtattat ggcgatgaag tggccctgtt
     1321 cgataaagac gatcggcaaa gtaatcctca tgccataacc gctggactta actatacccc
     1381 cttcccgctg atgaccttca gcgcggagca acgccagggt aaacagggcg aaaatgacac
     1441 ccgttttgcc gtcgatttta cctggcaacc tggcagcgca atgcagaaac agcttgaccc
     1501 gaatgaagtc gctgcacggc gtagccttgc aggcagccgt tatgatctgg tggatcgcaa
     1561 caacaatatc gttctggaat atcgcaaaaa agaactggtt cgcctgaccc tgacagaccc
     1621 cgtgacaggg aagtcaggag aagtgaaatc actggtttcg tcgctacaaa ccaaatatgc
     1681 cctgaaaggc tataacgtcg aagccaccgc actggaagct gccggtggca aagtggtcac
     1741 aacgggtaaa gatattctgg ttaccctgcc ggcttaccgg ttcaccagta cgccagaaac
     1801 cgataacacc tggccgattg aagtcaccgc cgaagatgtc aaaggcaatt tgtcgaatcg
     1861 tgaacagagc atggtggtcg ttcaggcacc tacgctaagc cagaaagatt cctcggtatc
     1921 gttaagtacc caaacattga acgcggattc ccattcaacc gccacactga cttttattgc
     1981 gcatgatgca gcaggtaatc ctgttgtcgg gctggtgctc tcgacgcgtc acgaaggtgt
     2041 tcaggacatc accctttctg actggaaaga taatggtgac ggaagctata cccagatcct
     2101 gaccacagga gcgatgtctg gcacgctgac gctgatgcca cagctgaatg gtgtggatgc
     2161 ggctaaagcc cccgccgtgg tgaatatcat ttctgtttcg tcatcccgaa ctcactcgtc
     2221 aattaagatt gataaggacc gttatctctc cggcaatcct atcgaggtga cggtagaact
     2281 gagagatgaa aatgacaaac ctgttaagga acaaaaacag caactgaata acgcagtcag
     2341 catcgacaac gtgaaaccag gagtcactac agactggaaa gaaaccgcag atggcgtcta
     2401 taaggcgacc tataccgcct ataccaaagg cagtggactt actgcgaagc tattaatgca
     2461 aaactggaat gaagatttgc ataccgctgg atttatcatc gacgccaacc cgcagtcagc
     2521 gaaaattgcg acattatctg ccagcaataa tggtgtgctc gccaatgaga atgcagcaaa
     2581 caccgtctcg gtcaatgtcg ctgatgaagg aagcaaccca atcaatgatc ataccgtcac
     2641 gtttgcggta ttaagcggat cggcaacttc cttcaacaat caaaacaccg caaaaacgga
     2701 tgttaatggt ctggcgactt ttgatctgaa aagtagtaag caggaagaca acacggttga
     2761 agtcaccctt gaaaatggcg tgaaacaaac gttaatcgtc agttttgtcg gcgactcgag
     2821 taccgcgcag gttgatctgc agaagtcgaa aaatgaagtg gttgctgacg gcaatgacag
     2881 cgtcacaatg accgcgaccg tccgggatgc aaaaggcaac ctgctcaatg acgtcatggt
     2941 cactttcaat gttaattcag cagaggcgaa actgagccaa accgaagtga atagccacga
     3001 cgggatcgcc acagctacgc tgaccagttt gaaaaatggt gattataggg ttacggcctc
     3061 tgtgagctct ggttcccagg ctaatcaaca ggtgaatttt atcggtgatc aaagtactgc
     3121 tgccctgacc ctcagtgtgc cttcaggtga tatcaccgtc accaacacag ctccgcaata
     3181 tatgactgca accttgcagg ataaaaatgg caacccacta aaagataaag aaatcacctt
     3241 ctctgtgcca aacgacgtcg caagtaagtt ctcgattagc aacggaggaa aaggcatgac
     3301 ggatagtaac ggggttgcaa tcgcctccct gaccggcacg
//
LOCUS       DAEVZA010000264         3268 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEVZA010000264 DAEVZA010000000
VERSION     DAEVZA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.264"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..233)
                     /locus_tag="LAF92_005069"
     CDS             complement(<1..233)
                     /locus_tag="LAF92_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HBI9793947.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVV"
     gene            complement(237..929)
                     /gene="tolQ"
                     /locus_tag="LAF92_005070"
     CDS             complement(237..929)
                     /gene="tolQ"
                     /locus_tag="LAF92_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBI9793948.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(926..1330)
                     /gene="ybgC"
                     /locus_tag="LAF92_005071"
     CDS             complement(926..1330)
                     /gene="ybgC"
                     /locus_tag="LAF92_005071"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBI9793949.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(1480..1773)
                     /gene="ybgE"
                     /locus_tag="LAF92_005072"
     CDS             complement(1480..1773)
                     /gene="ybgE"
                     /locus_tag="LAF92_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBI9793950.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(1773..1886)
                     /gene="cydX"
                     /locus_tag="LAF92_005073"
     CDS             complement(1773..1886)
                     /gene="cydX"
                     /locus_tag="LAF92_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBI9793951.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(1901..3040)
                     /gene="cydB"
                     /locus_tag="LAF92_005074"
     CDS             complement(1901..3040)
                     /gene="cydB"
                     /locus_tag="LAF92_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBI9793952.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(3056..>3268)
                     /locus_tag="LAF92_005075"
     CDS             complement(3056..>3268)
                     /locus_tag="LAF92_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="part of the aerobic respiratory chain; catalyzes
                     the ubiquinol to ubiquinone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I ubiquinol oxidase subunit CydA"
                     /protein_id="HBI9793953.1"
                     /translation="IGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKF
                     ARLGPSSLKTGRYHFEQSSTTTQPAR"
BASE COUNT          890 a          882 c          804 g          692 t
ORIGIN      
        1 accaccacgg tgtactgacc aataccagac acttcaacaa tcactggcgg attatcgtta
       61 ctgctcaccg cctgtgattc agtagcgtct ggcagatcga cctccacgct ctgggtgatg
      121 atgggcgctg tcgccataaa gatcagcaac agcaccagca gtacgtccag caacggtaca
      181 atgttgattt cggacttgag atcgcgacga cctcgtccac gcgctctggc catggcttac
      241 cccttgttgc tctcgctaac ggtaaacgcc tggcggtgca gaatcgcggt aaactcttcc
      301 ataaagttgt cgtaattcag ttccagtttg tttacgcgct ggttgaggcg gttataagcc
      361 attaccgccg gaattgcggc gaacagacca attgcagtcg caatcaacgc ttctgcgata
      421 ccgggcgcaa ccatttgcag tgttgcttgt tttaccgccc cgagggcgat aaaggcgtgc
      481 atgatccccc agaccgtacc aaacagacca atatacgggc tgatggagcc aaccgtaccg
      541 aggaacggaa tgtgcgtttc cagattttca agttcacggt tcatggagat acgcatagca
      601 cgcgacgccc cttccactac ggcttccggc gcatggctat tggcacgatg caggcgcaca
      661 aactctttga acccgctgta aaagatttgt tccgaaccag tcagattatc ccgtttcccc
      721 tggctctctt gatagaggcg agagagttcg attccagacc agaatttatc ttcaaacgct
      781 tcggcttcgc gcgccgctgc gttaagaata cgggtccgct ggataataat ggcccaagat
      841 gcgattgaaa aaccaatcaa aatcaacatg ataagtttaa ccagaaggct agccttcagg
      901 aacaaatcaa ggatattcat gtcagtcact gcttaaactc cgcgacaata gacttgggaa
      961 gcgcacgagg cttcattttg agtgggtcaa cgcaaacaac cagaacctct gcttcattca
     1021 gcaaagtatt ctcggcgttg acaatacgtt gcgtgaaaac caaagaggtg ccacgcattg
     1081 atgttatttc agtctgtatt tcgagcatat cgtcgagccg cgcaggtgcg taatattcca
     1141 ccgtcatttt acgtaccaca aaggcaacgc gttcagccat cagcgcctgc tgactgaagt
     1201 gatgatgacg cagcatctct gtgcgtgctc tttcataaaa agcgacgtaa ctggcgtggt
     1261 acaccacacc accggcatcg gtatcttcat agtagacgcg aaccggccat cgaaacagcg
     1321 ttgtattcac tttacatccc ggtaatgcaa caaaagttag agcttttaaa cgctgctact
     1381 atacgcgcgc gattgaggtt tgggaatgag ggcaagttaa gggagcgtaa aaatttatgg
     1441 gcctggagcg gcccataatt ttttaaagag aattctgatt taaaagaaga agaaaatcag
     1501 cccgacaatg agaacaatat cggcaagcaa tgggcaaaaa atcccttgcc aaagaacctt
     1561 ctgcggacga aagcccacgc cgtgaatcac accagcacag acggcccaca tcagcaataa
     1621 accatgccag atttccagtt cactggtctt cgcggcaaaa cgagatgggt cccaaaacat
     1681 acatcctgct aacagaagcg ccatcacgaa ggaaagcgcc cgcagggggc gcttgtccat
     1741 taccgcatac aaagtcgcga taatcttact catcagatgt cttcttgacc ggctttgcct
     1801 gattcgacgt gttcaagcgc cagcgcggtg attaccccaa acgaacaggc aagaagcgtt
     1861 cccagaatcc atgcgaaata ccacattttt agctccttac ttagtacaga gagtgggtgt
     1921 tacgttcaat atcttctttg gtgatacgac cgaacatttt ccagtaacac caggcggtgt
     1981 agagcagaat gatcggtacc agaaccaccg caacccaggt catgacgtta agcgtcagct
     2041 ggctggaagt tgcatcccac attgtcagac ttgcgttcat catggtgctg gacggcatca
     2101 caaacgggaa cattgcgata ccggctgtca ggatgatgca ggccagcgtc agggaggaga
     2161 acacaaacgc ccacgcggct ttatccatac gtgcagtcag gatggtcagc agcggcagaa
     2221 ccacacccag tgccggaata gcccacagaa ttggcgtgtt gttgaagtta accagccatg
     2281 cgccagcttc acgaaccact tctttattca gtgggttaga ggctgcgtaa tggtccattg
     2341 tcgatttcac gacataacca tcgataccgt acatcaccca tacgccagcc agtgcgaaac
     2401 agaccagtgt caccagtgca gccacctgag ccgttgcacg ggtacgcagg tgcagttcgc
     2461 ccacggtacg catttgcaga taggttgcgc cctgagtaat gatcatccct acgctcacca
     2521 cgcctgccag caggccgaac gggttaagca actggaagaa gttaccggtg tagtacagac
     2581 gcagatattc atcaacgttg aacggtacgc cctgcaacag gttaccgaac gctacaccaa
     2641 tcaccaacgg cggaacgaag ctaccaatga agatgcccca gtcccacatg ttacgccagc
     2701 gggtttcttc aatcttggag cggtagtcaa aaccgaccgg acggaagaac aaggacgcca
     2761 gcacgaggat catcgccaca tagaagccgg agaacgcagc agcatagacc atcggccagg
     2821 cagcaaagag tgcgccgccc gcggtgatca gccaaacctg gttaccgtcc cagtgtggtg
     2881 caatggagtt aatcataatt cgacgctcgg tgtcgttacg accgaggaaa cgggtgagca
     2941 tgcccacccc catgtcgaaa ccgtcagtga ctgcaaaacc aatcagcaga acgccaacca
     3001 gcagccacca gataaaacgc aatacttcat aatcgatcat ttgacgactc ctgtcttagc
     3061 gtgccggctg agtagtcgtg gaagactgct caaagtgata gcgaccggtt ttcaggctgc
     3121 ttgggccgag gcgtgcaaat ttgaacatta agaacaattc tgccaccagg aacagggtat
     3181 acaggccgca aatcagcacc attgagaaga tgagatcgcc tgcggtcagt gacgagttcg
     3241 ccacagctgt cggcagcact tcaccgat
//
LOCUS       DAEVZA010000265         3241 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEVZA010000265 DAEVZA010000000
VERSION     DAEVZA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.265"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1..1984
                     /locus_tag="LAF92_005076"
                     /pseudo
     CDS             1..1984
                     /locus_tag="LAF92_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            1969..2166
                     /locus_tag="LAF92_005077"
                     /pseudo
     CDS             1969..2166
                     /locus_tag="LAF92_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            2182..2391
                     /locus_tag="LAF92_005078"
     CDS             2182..2391
                     /locus_tag="LAF92_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793954.1"
                     /translation="MNEAKEKDLGTYKKSTLKTEKITRGLFSNDEITLIYFSEYSKRI
                     VQEVFVFNVEDKKVKLKGYRYDSIN"
     gene            complement(2413..2532)
                     /locus_tag="LAF92_005079"
                     /pseudo
     CDS             complement(2413..2532)
                     /locus_tag="LAF92_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2899..3241
                     /locus_tag="LAF92_005080"
                     /pseudo
     CDS             2899..3241
                     /locus_tag="LAF92_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          900 a          696 c          912 g          733 t
ORIGIN      
        1 gtgaaggcca ccgtgtcgct gtccactacg gctacgacga taagggccgc ctgacgggcg
       61 aacggcagac ggtggagaac ccggagacgg gggaactgct gtggcagcat gagacgaaac
      121 acgcctacaa cgaacagggg ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg
      181 agtggctgac ctacggcagc ggttacctgg cgggcatgaa gctgggcggg acgccgctgg
      241 tcgagtatac gcgggacagg ctgcaccgtg agacggtgcg cagcttcggc agcagggcag
      301 gcagtaatgc cgcatatgaa ctgaccagca catacacccc cgcaggccag ttacagagcc
      361 agcacctgaa cagcctggtg tatgaccgtg actacgggtg gaatgacaac ggcgacctgg
      421 tgcgcatcag cggtccgcga cagacgcggg aatacggcta cagcgccacg ggcaggctgg
      481 agagcgtgcg caccctcgca ccggacctgg acatccgcat cccgtatgcc accgacccgg
      541 cgggaaaccg cctgccggac ccggagctac acccggacag cacgctcacg gcgtggccgg
      601 ataaccgcat cgcagaggat gcgcactatg tttaccacta tgatgaatac ggcagactga
      661 cggagaagac ggaccgtatc ccgacgggtg tgatacggac ggacgacgag cggacccacc
      721 actaccacta cgacagccag caccgcctgg tgttccacac gcggatacag catggcgagc
      781 cactggtcga gagccgctac ctctacgacc cgctgggacg gcgaatggcg aaacgggtat
      841 ggcggcggga gcgtgacctg acggggtgga tgtcgctgtc gcgtaaaccg gaggagacat
      901 ggtacggctg acgacggtac agaccgacac cacgcgtatc cagacggtat accagccggg
      961 gagcttcacg ccgctcatcc gggtcgagac agagaacggc gagcgggaga aagcgcagcg
     1021 ccgcagcctg gcggagaagt tacagcagga agggagcgaa gacggtcacg gtgtggtgtt
     1081 cccggctgaa ctggtgcggc tgctggacag gctggaggaa gaaatccggg cagaccgcgt
     1141 gagcaatgaa agccgggcgt ggcttgcgca gtgcgggctg acggtggagc aactggccag
     1201 acaggtggag ccggaataca caccggcgcg aaaagttcat ttttaccact gcgaccaccg
     1261 gggcctgccg ctggcgctca tcagcgaaga cggcaatacg gcgtggcgcg gggagtatga
     1321 tgaatggggc aaccagctta atgaagagaa cccgtattac ctgcaccagc cataccgtct
     1381 gccggggcag cagcatgatg aggaatcagg gctgtactat aaccggaacc ggtactatga
     1441 cccgctacag gggaggtata ttacacaaga ccccattggg ctggcggggg gatggaacct
     1501 gtataattac ccactgaatc cgataataag gatggatcct ttgggtttgt ataatttata
     1561 tcaattatta tatgatgttt ggcatgatga ttcatatgga acatcatcaa ttgatattac
     1621 tggcagtgga gatctaatat cattaggtgg tcatgcagga cttggcgttg cgtttgctaa
     1681 aaagaaaggt gaaatgttat ctgatatttg tatttatgct acagcatgcg gacatgcagg
     1741 aattggtggt gggataaatg cggctatcac atattctgag accaagtctt tacctacatc
     1801 gggagtcagc aattcagtag gtgtaacggt tggcggcgga gttggggggc attttgcgta
     1861 tacttatgta gtggatgttg ataatccaga atcatcgaca gaatctgttg gtatcggtgc
     1921 aggtgttgac gcttcagtta tgactctggc ttgtagaacg tggcaagaat gctgggtcaa
     1981 ttaacaggag gaaatatgaa aaaatatata tttttagttt tatttttatt gtgtggttgt
     2041 tctccagtgg acatagagcc agctagacaa gaagttaata tctttcgtgg tttatatcag
     2101 acagggaaat acagtgatat atataaaatc acttcaagtg attttcagac tgccacatct
     2161 gaaaaaaaat ttatcgacat aatgaacgaa gcaaaagaaa aagatcttgg gacttataaa
     2221 aaatcaaccc ttaaaactga aaaaatcact cgcggattat tttcaaacga tgaaattaca
     2281 ttgatatatt tctcagaata ttcaaaaaga attgtccaag aggtttttgt gtttaatgtt
     2341 gaagacaaaa aagtaaaatt aaaaggttat aggtatgatt cgattaatta aattttttga
     2401 tgatgacttc aattagtaga acatgtattt ttcgataaaa gcaccgatga ctttttcatg
     2461 aagttctaca gagcgcgaga aacagatggt tctgcgagtc agacgtttga ttcaggttct
     2521 caggggcaga ttcagggaaa gatcacaggg aaagatcatc ggtgtgccgt aggcacaaag
     2581 atgcaaaacg ggacgttttg catgtaataa gcataagctt gtgaagagat ggtggcaggc
     2641 ccaccgggat ctgctgtcgg aagtcggtcc caaacaaccc cggctgctgt ggtaaagagc
     2701 catggtagtg agcgcagggg gaacgatcag acgagatctg atcctatgtg agattaggga
     2761 aagatcatca aaagttcact ttttgtacta aataattcgc attttatgtt taaaaattga
     2821 gatattcctc attacctgaa gctgtttttt attgcttata catgatcaaa tactccttac
     2881 ataattaagg agaacaaaat ggaacttaaa aaattgatgg aacatatttc tattatcccc
     2941 gattacagac aagcctggaa agtggaacat aaattatcgg atattctact gttgactatt
     3001 tgtgccgtta tttctggtgc agaaggctgg gaagatatag aggattttgg ggaaacacat
     3061 cccgatgttt tgaagtgata tggtgatttt gaaaatggta ttcctgttca cgatatcatt
     3121 gccagagttg tatcctgtat cagtcctgca aaatttcacg agtgctttat taactggatg
     3181 cgtgactgcc attcttcaga tgataaagac gtcattgcaa ttgatggaaa aacgctccgg
     3241 c
//
LOCUS       DAEVZA010000266         3193 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEVZA010000266 DAEVZA010000000
VERSION     DAEVZA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.266"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(211..807)
                     /locus_tag="LAF92_005081"
     CDS             complement(211..807)
                     /locus_tag="LAF92_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793955.1"
                     /translation="MMKSDEQYHVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKVLSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            complement(804..1523)
                     /locus_tag="LAF92_005082"
     CDS             complement(804..1523)
                     /locus_tag="LAF92_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBI9793956.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(1520..1954)
                     /gene="psiB"
                     /locus_tag="LAF92_005083"
     CDS             complement(1520..1954)
                     /gene="psiB"
                     /locus_tag="LAF92_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBI9793957.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFSVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(2009..>3193)
                     /locus_tag="LAF92_005084"
     CDS             complement(2009..>3193)
                     /locus_tag="LAF92_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793958.1"
                     /translation="ELRTDLFSDDGDGYVDRVALDAALLEKLQAVAEHLREAEGWEWC
                     AGRMEPVGECREDAGTYRCLPEPEAVLTEAEDERLNELMTRYDALENQCEESDLLEAE
                     MKLMRCMAKVRAWTPEIRAGSGVVVSWRYGNVCVQRGVQLRSEDDATDDADRTEQVQE
                     KASVEEISLPLLTKMSSERTLAVQAALMQQPDKSLALLAWTLCLNVFGSGAYSKPAQI
                     SLECEHYSLTSDAPSGKEGAAFMALMAEKSRLAALLPEGWSRDMTTFLSLSQEVLLSL
                     LSFCTACSLNGVQTRECGHTSRSPLDSLESAIGFHMRDWWQPTKANFFGHLKKPQIIA
                     ALNDAGLSGAARDAEKMKKGDAAEHAEHHMKDNRWVPGWMCAPHPQTDATERTDNLAD
                     AA"
BASE COUNT          677 a          985 c          833 g          698 t
ORIGIN      
        1 gatggaggca cggcacagcc cgcacacgac gctgaggcca cagagctgaa aacccacaag
       61 gccatgcccc gcatggccgg gtaaaagcgg agccgccggc gacttcctta ttccggcgag
      121 tgcagaggac acgccgcatg acctccagac cacccttgat atgaaacgat catgatcggc
      181 gtgtccctgc gtaaaatata catgtctggt tcaccggaga atatctgtga tatccacaag
      241 ttttctgtcg cacagccagt cctgcgcttc acgcaccacg ctactgtcgg gtttcaccgc
      301 tttcagcgat gatgccagtc gcttgatcaa cataaccagt tcccgtgtct gtatgtccgg
      361 cattgtctcc ggcggagcag agggactctc atttccgacg cccatcccat gcgcttccag
      421 tgtggcacgg acaaaccagg cggcatcgac cagttgctgg cgaatccagg tcagatcggc
      481 atttctgtca cgggctgcca ttcgctcacc cgacagcacc ttgctgatat ggctgcgcac
      541 cagattatcc aggagttcct gcgcttcttc cggtgaaaag ccacctggta tctctgtgcg
      601 caacagtcct ttcctgtaca gtagctgcag cagatcaatc tgcgaccgca ctgcaactat
      661 cagtacataa cggacaatat tcgcactggc ggtggtttct gtatcattca gcagttcctc
      721 cggatcatta ccaccggtat tgcagagcaa cggcaacaga ggtcgcatcc acacgggaac
      781 gtgatattgt tcgtctgatt tcatcatatt gcgttctcca gcttgttcgg gatcatccac
      841 tgctcaagtg cacgtacctg taccggtgca ccacgaccgg cctcaccggc ttcaaaaatc
      901 agccgccaga gtggttcctc ctgccagaaa aaccggtcaa atgatgacag tgatggaaaa
      961 cgcccgcacc acccccagaa aatcgtttcc agatcatgct cctcaacaac accagaccag
     1021 cgggacaacc agcttccgac cgtggcggga gtatgaaacg caaggtcaat ctgcgcctga
     1081 gcaaaacatg tctggtattc cagttcccgt cgttctgcct cacgttcccg ctggcggctg
     1141 tatttctgct ctgtcagcgt ggttttctga cgacgccgct caccttcacg aaaaggaacg
     1201 gacgggaaca gacgatgccc cgcatcggat ggcaggggaa gaacacagac ttcaccaaaa
     1261 ctttcaatca gtttatccac ggcagccagc cacaggagac ggttgccatg aaattcctcc
     1321 ttcgtcagcg caggagcaaa aaagcggaga tcggtcagcg aaatccggcg actgccattc
     1381 aggcagcgaa agaaagcgct ggcataaggc catgctgaaa gtgtgcgtcc ctgacgacga
     1441 cgttgttcgg tgacagccac agcctgcatg gcggcctgtt gctctgcgct gagggggatc
     1501 agtgcccgtg aacgtgcgct catgcctgac ctccttctgc catcaggata tggatgatgt
     1561 cgttaacact gtacccctgt gcatcaagtg tcgcggcaag cgccagcgtg tgactgatag
     1621 cgacaggcgt gaagatatgt tgcacctgaa cgacggagaa aggctgtcca ccacaattca
     1681 ccagaaccag catccagagc tgagagacgt cacccggtga acataacgcc aggtgaaaac
     1741 gttcgtgggc tggcgtgaaa cgaacctgga cggggaaaaa gcccccgaac tcactgccat
     1801 actcgccatt catcatccag ttagccggtg cgtccacttc acgcatcacc tcgtgagtga
     1861 gattacggcg catatcgctt cccgcagcac ggatatcttc atattcctgt gcgttcatgg
     1921 actgcagggc attcagggtc agttcagttt tcatcacggt atctcccttg ctgccgcccc
     1981 gtctccggcg gggcggtgtg gttgttcatc aggcggcatc agccaggtta tcggtgcgtt
     2041 cagtggcatc tgtctgtgga tgtggtgcac acatccagcc tggaacccag cggttgtctt
     2101 tcatatggtg ctctgcatgt tcagccgcat cacctttctt catcttctct gcgtcccgtg
     2161 cggcaccgga cagtccggca tcattcaggg ctgcgataat ctgcggcttt ttcaggtgtc
     2221 cgaagaagtt tgctttcgtc ggctgccacc agtcgcgcat gtgaaaaccg atggcgctct
     2281 ccagtgagtc aagcggactg cgtgacgtgt gaccacactc gcgggtctgg acaccgttaa
     2341 gactgcacgc ggtacagaag ctgagcaggg ataacagcac ctcctgactg agggacagga
     2401 atgtcgtcat gtcccgtgac catccctccg gcagcagggc tgcaagacgg gatttttctg
     2461 ccatcagcgc catgaatgcg gcaccttcct tccccgatgg cgcatcgctg gtcagcgaat
     2521 aatgttcaca ttccaggctg atttgtgctg gtttactgta cgccccgctg ccaaacacat
     2581 tcaggcagag cgtccatgcc agtagtgcca gagatttgtc cggctgctgc atgagtgccg
     2641 cctggactgc cagcgtgcgt tcggaagaca ttttcgtcag caacggcagg ctgatttcct
     2701 ccactgacgc tttctcctgc acctgttccg tgcggtcagc gtcgtcagtc gcgtcatctt
     2761 cactgcgcaa ctgcacacca cgctggacac atacgttgcc ataacgccag gacaccacca
     2821 caccgcttcc ggcacgtatc tccggcgtcc acgctctgac cttcgccatg cagcgcatca
     2881 gcttcatttc tgcttccagc aggtcggatt cctcacactg gttttccagc gcgtcgtaac
     2941 gcgtcatcag ttcgttcagg cgttcgtctt ccgcctccgt cagcaccgct tccggctccg
     3001 gcagacagcg gtatgttccg gcatcctcac ggcactcacc gacaggctcc atgcgcccgg
     3061 cgcaccattc ccagccttcg gcttcccgaa ggtgttcagc gacagcctgg agtttttcca
     3121 gcagggcggc atcgagcgcc acgcggtcga cataaccgtc cccgtcatcg ctgaacaaat
     3181 cggtgcgcag ttc
//
LOCUS       DAEVZA010000267         3192 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEVZA010000267 DAEVZA010000000
VERSION     DAEVZA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.267"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(23..679)
                     /locus_tag="LAF92_005085"
     CDS             complement(23..679)
                     /locus_tag="LAF92_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI9793959.1"
                     /translation="MSEIQRLYIVDDDESVLDSLEFVLQGEGYQTQCFSSAEAFLQWS
                     ERHIECLTDTGCLILDCRMPGMGGQTLQTLLSQTRSTLGIVFLTGYGDVPMAVDALKQ
                     GAVDFLQKPVDQKHLLRAVKNALIWSAAEKARQTYISRYEQLTGRERNILTLLADGKT
                     NQQIAENLFISARTVEVHRANMQKHLGMGSLAEMVRLYTLVEPYLGEICEPPVRIKRK
                     "
     gene            complement(691..2502)
                     /locus_tag="LAF92_005086"
                     /pseudo
     CDS             complement(691..2502)
                     /locus_tag="LAF92_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
     gene            complement(2711..3118)
                     /gene="cadR"
                     /locus_tag="LAF92_005087"
     CDS             complement(2711..3118)
                     /gene="cadR"
                     /locus_tag="LAF92_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HBI9793960.1"
                     /translation="MRIGQLAQLVGVETQTIRFYEQQGLLPPPDRQDNGYRVYTEKHG
                     EGLAFIRRCRILGLSLAEIHELQSYQDAPHQPCTAVNALLDDHISHVRSQITALQALE
                     KQLVSLRASCNDDREVEACGVLAGISEGNMHQQ"
BASE COUNT          832 a          735 c          690 g          935 t
ORIGIN      
        1 atagccgtat tctatttctc ttctatttcc gttttattct gaccggcggc tcgcaaatct
       61 cacctaaata aggttcaacc agcgtataaa gacgaaccat ttcagccaaa gaacccatgc
      121 ctaagtgttt ttgcatattg gctcgatgta cttcaaccgt tcgggcagaa ataaaaagat
      181 tttcagcaat ttgctgattt gtttttccat cggccaacaa agttaagata ttgcgttcac
      241 gaccggttaa ttgctcataa cggctgatgt acgtttggcg cgctttttct gctgctgacc
      301 aaattaaagc gtttttcacg gcacgtaaca gatgtttttg atcgaccggc ttttgcagaa
      361 aatcaacggc tccttgtttc aaggcatcaa cggccatcgg aacatcgccg tatccggtca
      421 gaaaaacgat acctaaagtg cttctggttt ggctaagcaa tgtttgcagc gtttgaccac
      481 ccattcccgg catgcggcaa tccagaatta agcaaccggt gtccgtcagg cactcaatat
      541 gccgttcact ccactgtaaa aaagcttcgg cggaggagaa gcactgagtt tgatatcctt
      601 caccttgcag aacaaattca agggaatcca gaacggattc atcatcatca acaatgtata
      661 aacgctgtat ttctgacatg ctgaactccg ttatttatgg ttgtggtaga gagtgaagat
      721 aacagatgct tcgcatccgc cgtcaggatg gttttgtagt aaaaatgtac tgcgatgtga
      781 ctcggcaatt tctttacaga taactaaacc aaggcctatt ccgtccgctt ttgttgtgac
      841 aaatggcgtc ataagttctt ccagtggcag ttgtaaacct gctccgcgat ctctgacggt
      901 taaatgtgcc ttgttttctg tatgtaacag attgatcgat acgctgcaag caccggcctg
      961 agcggaagca tcaattgcat tagtaaataa attaaccatt aattgttcta ttcctgcttt
     1021 atctgccttc agaagcatct tttgcttatc gatatgtaaa attagctcta cttgttttga
     1081 ttttgcatag atctcaacaa gttttattgc ctgctgcaga ataattgcca gatcctgagg
     1141 cgttttttgt atagcctgct ttctgagtaa ttgacggaaa cgatcgacaa cttgattaat
     1201 tcggataacc tgatggcgaa ttttatccag taacacatca atatccgtgg agtggtcacc
     1261 gcgttttcgg cgcgatagtt caccttcagc atacagatga atcgcggaca gcggctgact
     1321 taactcatgc gctaaacttg agcccagttt attgaccaat gcccgacgtt gagcatattg
     1381 cagattctct aacgcttgct gtagcgaaag ggtactttta cgatatttca tcgtcaggaa
     1441 aatgtattga agcggcaaaa taattaaggc gataccgatg acgatcagca ggtagtaacg
     1501 gctcttaatc cactcccata atttaatcca ccagggctgt tccagaggat gtattcctaa
     1561 atctttaaac aatcgatcca gttccgcctg actttcagga actgtccatc ccagagattg
     1621 tgcagctttt gcgacttcgg attggctagg aaccgctaat aatgcggtga gaatctttcg
     1681 tcccaacgtt ctgtctgccg cgtcggtcat ggcaaaactc cagttaggat aaagacgagt
     1741 agaaacctga cagccaaagg agtcgggcgc gatattgttt aatgcccgga gtgctttttc
     1801 ttccaactgg tgtttttcta tcattgattc aacgaggcag accggcaaaa tagcggcctg
     1861 aattttcccg gctaataaag cctgaacaac ggattcatga ggataaccga tttcctgtac
     1921 atgagagaaa taacgattgc tgacacccat ttggtgcaac tctctttcca ttgccataaa
     1981 accaccgaat gcctgacgat gagcggtacc gatgggctta ccggataact gtaatagatc
     2041 ggtaaacggg ctattttctt tgacccacat agccgaacct gttgcggtag ttgttccgcc
     2101 ctgcattggg ctgattaatg tcgccatcca ggataaagag tgaaccctac cgatacggat
     2161 actttctgat ggattaacaa tgataaattg cagattttgt tgactaattt cgtgctgtaa
     2221 ctgagataaa ggcagcggaa ttaaccggaa atcatactct ggtatttctt gttttaacca
     2281 attgattgtt ggttgccatc tttctctggc ttcagtcatt ccccgatcgg caaaaacacc
     2341 aatagaaatc agatgctttg ttggctcgtt taccggttct gcactgataa ctgcgatgga
     2401 tttcatcata aaaaaaatga gcattccaaa atatatcaat gacttcatta tgctgtttcc
     2461 tgtttttatc tgtcatgaaa gatatttctt ggtgtcaggc atttgcttac atggttaatt
     2521 tctgaaaacg taaatcagtc tttcaagctt atcgctatcg gcatatctgc ttgccgccgg
     2581 tctgatgtta ttctttctgc tattatctag agctatatta taggggtaag cggattaaat
     2641 ggttgatctt acctttgcat gagagtgaga cagaccggca tctcatcgga ttatctggtt
     2701 gatgcttcac ctactgctgg tgcatgtttc cttcgctaat tccagcaaga accccacacg
     2761 cctcaacttc ccggtcatcg ttgcaactcg ctctcagtga aacgagttgt ttctcaagcg
     2821 cttgcagagc ggttatctgc gaccgcacat gagagatgtg atcatcgagc aaggcgttga
     2881 cggcggtaca aggctgatga ggggcgtcct gatagctctg tagttcgtga atctcagcca
     2941 gtgacaggcc caggattctg cagcgacgga tgaaggccag cccctcacca tgcttctcgg
     3001 tatagacacg gtaaccgttg tcctgccgat caggcggcgg caacaagccc tgctgttcat
     3061 agaagcggat cgtctgtgtt tcgaccccta ccaactgcgc caactgacca atgcgcatca
     3121 gcctcctccc caacggattc tttactctat tgaccttata gtagctttat agttttaaat
     3181 ggtaccacaa ca
//
LOCUS       DAEVZA010000268         3140 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEVZA010000268 DAEVZA010000000
VERSION     DAEVZA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.268"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(67..1164)
                     /locus_tag="LAF92_005088"
                     /pseudo
     CDS             complement(67..1164)
                     /locus_tag="LAF92_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(1221..2189)
                     /locus_tag="LAF92_005089"
     CDS             complement(1221..2189)
                     /locus_tag="LAF92_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="HBI9793961.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            complement(2224..3099)
                     /locus_tag="LAF92_005090"
     CDS             complement(2224..3099)
                     /locus_tag="LAF92_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-65"
                     /protein_id="HBI9793962.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAVAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAERR
                     RDVLASAARIIAEGL"
BASE COUNT          639 a          923 c          831 g          747 t
ORIGIN      
        1 caggaatcaa cgcctttacc cgctgccgga gcctcacctc aatagcactc tccggcagcc
       61 gtattgttac agcgaatcaa actgatagct gaccgatagc ccaacgccat agttacggcc
      121 cggcgccggt tcataatagc gtccgttgct ctcgttgacg ataacagagc cgacgtagcg
      181 cttatcaaac aggttatcaa cgcgcgtata gaggtcgacg gtccagttat ctagcacata
      241 tttatagccg gtattcagtg ccgtcaccgt ataggctgga gcctgctcgc tattggcatc
      301 gttgacctga atatcgctca tatagcggac ttcagcgcca gcgtaccatc cctcttccgg
      361 cacccagcca aaggaggcat aggcgctatt acgtgcaatc cccgggatcc gattgccgct
      421 ctgaatagca tcgccggcgt tttcacggta ggtcgcatcc agcagcgtcc acgccagttt
      481 ggcgcgccag gcgggcgcaa attgctgatc ccatcccagt tcgaccccgc ggcggcgcgt
      541 cttgcccgcg ttctgatagg acgtacggcc attgtcgctg gcggcaacga caatttcatt
      601 tttcgtgtcc gtattgaaga cgctcaggct cgccatcccg gtgcccacct gccatttact
      661 gcctgcttca acggtggtgt tagtggccgg ttgcagagca aagttaagcc cggactgacc
      721 atcgggacga taggacagct cgttaatggt cggcgtttca aatcccctcc cggcagagag
      781 gtaaacgttc cactgcggcg tcatggcata cttcagcgct gccgccggaa gccacttgtg
      841 atagctcgct tcgccgctat catcaccatt tttacctaca acataacgat cgttggaatc
      901 aaaccatacc gagctatagc gcgccccggc gtccagcccc agggctgagg tgagctgcca
      961 gtgcgtttga atgtagggat cgaggttcca catcaggtta cgttcgttac gtcgcatatc
     1021 acctttggtg ccgagatcgt aaacgccgtt ctgataaaca aagttctcat agcctttgcg
     1081 ctgttcggtc atcgcctcgt aatcaaggcc ggtggtgatg gcgtaaggga taaaaccgct
     1141 gtcatcgtgg tgtgtccagc gcgtggcttt gttgaataaa tcagatttcg ggtaagtctc
     1201 ccccgtagcg ggttgtgttt tcaggcaata cgcacgcttt caggcatacc tgctttcgtc
     1261 attttgttca gcgctcgtac cagggccata gcctccgcaa cctgaccatc gtagtcacgc
     1321 agcgtcagtg aacccccgaa cagctgtttt acccggtaca tcgccgtttc cgctatcgag
     1381 cgacggttgt aatctgttgt ccatttccac cgcgcattac tcccggtcat tcgctgatta
     1441 gccactgcac ggttacggtc tgcatattca ccgggccagt aacccgcacc ttttcggggc
     1501 gggataagcg cgctgatttt cttacgccgc agttcatcgt gacagagccg ggtatcgtaa
     1561 gctccatcgg cggcggctga cctgattttc cggtgggttt gccggattaa cccggggaag
     1621 gcctctgagt ccgtaacgtt gttcagcgac aggtcagcgc agatgatttc atgtgtttta
     1681 ctgtcaactg ccagatgcag cttacgccat atacgacggc gttcctggcc atgctttttg
     1741 actttccact cgccttcacc gaagaccttc agcccggtgg aatcaattac caggtgtgcg
     1801 atttcacccc gggtgggcgt tttgaaactg atattaaccg actttgcccg cctgctgaca
     1861 cagctgtaat ccgggcagcg tagcggaacg ttcatcagag aaaaaatgga atcaataaag
     1921 ccctgcgcag cgcgcagggt cagcctgaat acgcgtttaa tgaccagcac agtcgtgatg
     1981 gcaaggtcag aatagcgctg aggtctgcct cgtgaagaag gtgttgctga ctcataccag
     2041 gcctgaatag cttcatcatc cagccagaaa gttatggagc cacggttgat gagggcttta
     2101 ttgtaggtgg gccagttggt gattttgaac ttttgctttg ccacggaacg gtctgcgttg
     2161 tcgggaagat acgtgatctg atccttcaac tcagcaaaag ttcgatttat tcaacaaaac
     2221 cagttacagc ccttcggcga tgattctcgc cgctgaagcc agcacatcgc ggcggcgctc
     2281 tgcgttctgt tgcggctggg taaaataggt caccagaacc agcggcgcac gaccctgcgg
     2341 ccagatcacc gcaatatcat tggtggtgcc gtagtcgccg ctgccggtct tatcacccac
     2401 agtccacgac gtcggtaagc cggcccgaat gctggctgcg ccggtcgtat tgcctttgag
     2461 ccacgtcacc aactgcgccc gctgggtttc gcccagcgca tgacccagcg taagctgacg
     2521 caacgtctgc gccatcgccc gcggcgtggt ggtgtctctc gggtcgccgg gaatggcggt
     2581 attcagcgta ggttcagtgc gatccagacg aaacgtctca tcgccgatcg cgcgggcaaa
     2641 agccgtcacg cctcccgggc caccgagctg ggcaatcaat ttgttcatgg cggtattgtc
     2701 gctgtactgc aacgcggccg cgctcagttc tgccagcgtc attgtgccgt tgacgtgttt
     2761 ttcggcaatc ggattgtagt taaccagatc ggcaggcttg atctcgacag gctgattaag
     2821 cagctgcttt tgcgtttcac tctgcttaag caccgccgcg accgccataa ctttactggt
     2881 actgcacatt ggaaagcgtt catcaccgcg ataaagcacc tgcgtattat ctgcggtatc
     2941 gatgagcgcg acgcccagcc gccctccgct gcttttctcc agcgccgcca gcttttgctg
     3001 caccgcactc gtctgcgcat aaagcggcgc gctgcccagc agcagcggaa tgcacgccgc
     3061 cgccgcgaac atcatccgtt gcactctctt tgtcaccatc tcaaactccc aatacggtca
     3121 atccgtgtta catcagtatt
//
LOCUS       DAEVZA010000269         3123 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEVZA010000269 DAEVZA010000000
VERSION     DAEVZA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.269"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..438)
                     /gene="nuoB"
                     /locus_tag="LAF92_005091"
     CDS             complement(<1..438)
                     /gene="nuoB"
                     /locus_tag="LAF92_005091"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBI9793963.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVD"
     gene            complement(454..897)
                     /gene="nuoA"
                     /locus_tag="LAF92_005092"
     CDS             complement(454..897)
                     /gene="nuoA"
                     /locus_tag="LAF92_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBI9793964.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(1528..2466)
                     /gene="lrhA"
                     /locus_tag="LAF92_005093"
     CDS             complement(1528..2466)
                     /gene="lrhA"
                     /locus_tag="LAF92_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBI9793965.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT          879 a          763 c          667 g          814 t
ORIGIN      
        1 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcacc
       61 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
      121 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctgacgcg gcgaagcacg
      181 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
      241 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
      301 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
      361 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
      421 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
      481 cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
      541 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
      601 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
      661 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
      721 ggagccgacc gagtcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg
      781 accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag
      841 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
      901 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
      961 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
     1021 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
     1081 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
     1141 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
     1201 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
     1261 gatctgtgac acgcttttgt cattccataa caaaaacgca gcaacaaatt tacgtatttt
     1321 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
     1381 tctctggtga ttgaaaaatg aacagttttg tacgttctgc actatgcgag gaaggatttt
     1441 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg
     1501 tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc
     1561 atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc
     1621 ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc
     1681 ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat
     1741 catctcaacc ggccttgccg tcacaccaag accggctttc actgccgcac gaacggccgg
     1801 aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag
     1861 caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt
     1921 ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc
     1981 cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat
     2041 ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga
     2101 actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat
     2161 agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg
     2221 caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg
     2281 accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact
     2341 tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc
     2401 aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact
     2461 tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca
     2521 aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa
     2581 gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg
     2641 aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc
     2701 tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg
     2761 cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaggcgg catcaagagc
     2821 agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt
     2881 gcgaattatc tgatgcaaag ttatgttatg ttaggcaaag taatcttcta ttattaataa
     2941 gcacatcaaa acctttttga atattaaata ataattaatt agcatcatcc tcattcatta
     3001 attccgctta acaatagttt cacaccttgc ccccattacg acacctcgga aatcatcacg
     3061 ctgttaaact ctccgtaatg cggaaatatc atcatcgcgt ttcattagtg aattcttaaa
     3121 tga
//
LOCUS       DAEVZA010000270         3111 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEVZA010000270 DAEVZA010000000
VERSION     DAEVZA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.270"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..2484
                     /gene="traG"
                     /locus_tag="LAF92_005094"
     CDS             <1..2484
                     /gene="traG"
                     /locus_tag="LAF92_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBI9793966.1"
                     /translation="MVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIIN
                     LFQDYVQNCVLGDIYLNHKYTLEDLMASGDPYTLIFSRPSPLRGVYDSNNNFITCKDA
                     SVTLKDRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQI
                     MRQNVTMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQ
                     TILVGITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQ
                     NGAPVVLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAI
                     SPTASAAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGN
                     MVMDASGAMSRLPVGINATRQIAAAQQEMSREASNRAESALHGFSSSIASAWNTLSQF
                     GSNRGSSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTNA
                     SLGLYGDAYAKGHLGISVLGNGGGVGLQAGAKASIDGSDLDSHEASSGSRASHDARHD
                     IDARATQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMT
                     RSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAM
                     AWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDS
                     NIRNDVKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALTQNNKYN
                     EEKLAQERIPGADSPKELLEKAKSYQHKE"
     gene            2517..3038
                     /gene="traS"
                     /locus_tag="LAF92_005095"
     CDS             2517..3038
                     /gene="traS"
                     /locus_tag="LAF92_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632670.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HBI9793967.1"
                     /translation="MITQQIISSELEVLKKHIDSGDIRIPSLWQGLKPGFIIMGWMIF
                     CPLLMSFLITQKTSETLTAVLAGGWLGLIILFIVARIRMLYFSLPEEFLKTSSVMRII
                     SSKLKVYFIVYMGIIFLWSFLGGGIIYGFGAILVTVIMAFLIQLDIGRYQFVGVIDAI
                     NSYVKNKKLSRVK"
BASE COUNT          868 a          666 c          815 g          762 t
ORIGIN      
        1 atggtggcag gttatgagat ggtgttcgct cagcctgaca gcgccaccta cagcaagaca
       61 ggcatgctgt tcggggcgaa cctgattgtg aaaagcaccg atttcctgtc ccggaatccg
      121 gaaatcatca atctgtttca ggactacgtc cagaactgcg tgctgggtga tatttacctg
      181 aaccacaaat acacgctgga agacctgatg gcctccggtg acccctacac gctgattttt
      241 tcccggccca gcccgctacg gggcgtttat gacagcaata ataatttcat aacctgtaaa
      301 gatgcgtcgg tcacgctgaa agacaggctg aatctcgata caaagacggg aggcaagacc
      361 tggcattatt atgtgcagca gatatttggc ggcaggccgg accctgacct gttattcaga
      421 caactggtga gtgacagtta cagttatttc tacggctcca gccagtctgc cagccagatt
      481 atgcgccaga acgtcaccat gaatgccctg aaagagggta tcaccagtaa tgcggcccgt
      541 aacggtgata ccgccagcct ggtcagtctg gccacgactt cgtcgatgga gaaacagcgt
      601 ctggcacatg tctccattgg tcatgtgctg atgcgaaacc tgccgatggt gcagaccatt
      661 ctggtgggca ttaccattgg tatcttcccg ctcctggtac tggcggctgt cttcaacaga
      721 ctgacgctgt ctgttctgag aggctatgtg tttgctctga tgtggttcca gacctggccg
      781 ttgctgtatg ccattctgaa cagtgccatg acattctatg caaagcagaa tggtgcaccg
      841 gtcgtgctct ctgaactctc tcagatacag ctgaaatact ccgacctggc gtccactgcc
      901 ggatatatat ctgtcatgat acccccgttg tcatggatga tggtaaaagg ccttggcgca
      961 ggtttttcca gcgtgtacag ccatttcgct tcctctgcta tcagtccgac tgccagtgcg
     1021 gcaggtagtg tggttgacgg taattactcc tacggcaaca tgcagacgga aaacgtgaac
     1081 ggcttcagct ggagcaccaa cagcaccacg tcgtttggtc agatgatgta ccagaccggc
     1141 agtggcgcaa ccgccacaca gacccgtgac ggtaatatgg tgatggatgc aagcggagct
     1201 atgtcccggt taccggtcgg catcaatgca acgcgtcaga ttgcggcagc acaacaggaa
     1261 atgtcccggg aagcatcgaa cagagcagaa agtgccctgc atgggttcag cagcagtatt
     1321 gccagtgcct ggaacacgct cagtcagttt ggttctaacc gggggagcag tgattctgtc
     1381 acaggtggtg ctgacagcac catgagcgca caggactcca tgatggccag ccgtatgcgc
     1441 agtgcagtgg aaagctatgc gaaggcgcat aatatcagta atgagcaggc gacaagagaa
     1501 ctggcatcga gaagtacaaa tgcgtcgctt ggcctttacg gtgatgctta tgctaaaggg
     1561 catttaggca taagtgtatt gggtaacggt ggaggggttg gtctccaggc cggagctaag
     1621 gcaagcattg atggcagtga tcttgactca catgaagcca gtagtggttc acgggccagc
     1681 catgatgctc gtcatgatat cgatgccaga gctacacaag actttaaaga ggccagcgat
     1741 tactttacca gccgcaaagt cagtgaatcc ggcagccata ctgacaataa tgccgattcc
     1801 cgcgtggacc agctgtctgc agccctgaac tcagcgaaac agagttacga ccagtacacg
     1861 acgaatatga cccgcagcca tgaatatgct gaaatggcat cccgtactga aagcatgagt
     1921 gggcagatga gtgaagatct gtcgcaacag tttgcacagt atgtgatgaa acacgcaccg
     1981 caggatgccg aagctattct gacaaacacc agttcaccgg agattgcaga acgccgccgg
     2041 gcgatggcgt ggtcttttgt gcaggaacag gtgcagcctg gtgttgataa tgcctggcgt
     2101 gaatcccgtg gcgatattgg taaaggaatg gagagcgtac cttcgggtgg tggcagccag
     2161 gatattattg ctgaccatca ggggcatcag gccattattg atcaaagaac gcaggacagt
     2221 aatatccgga atgatgtaaa acatcaggtt gataatatgg tcacagaata taaagggaat
     2281 ataggtgata ctcagaacag tatccgtggc gaggaaaata ttgtaagagg gcagtattct
     2341 gaactacaaa atcatcataa aacagaagca ttaactcaga ataataaata taatgaagaa
     2401 aagctggctc aggaaagaat acctggtgct gatagtccga aagagctttt agagaaagca
     2461 aagagttacc agcataaaga ataatcgtaa catgttgggt agggtatgga gaggccatga
     2521 ttacacaaca aattatcagt agtgagttgg aagttttaaa gaaacatatt gactcaggag
     2581 atattagaat tccgtcacta tggcagggat taaaacccgg gtttataatc atgggatgga
     2641 tgattttttg tcccttacta atgtcatttc taataacaca aaagacaagt gaaacactca
     2701 ctgctgtgtt agccggagga tggcttggac ttattattct gtttattgtg gcaaggatta
     2761 gaatgttata tttctctctg ccggaagaat tcctaaagac atcatccgta atgaggatta
     2821 tatcatcaaa actgaaagtg tatttcattg tgtatatggg aattatattt ttatggtctt
     2881 ttttaggtgg agggattata tacggttttg gtgcaatttt agtgacggtt attatggcgt
     2941 tcttgattca attagatatc ggtagatatc aatttgttgg tgttattgat gcaatcaata
     3001 gttacgtcaa aaacaagaag ttatcaagag taaaataaaa gatattagag agtaaatata
     3061 tgatgaaaac aaaaaaacta atgatggttg cactggtcag ttccactctg g
//
LOCUS       DAEVZA010000271         3102 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEVZA010000271 DAEVZA010000000
VERSION     DAEVZA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.271"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1101)
                     /locus_tag="LAF92_005096"
     CDS             complement(<1..1101)
                     /locus_tag="LAF92_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmmdR/YeeO family multidrug/toxin efflux MATE
                     transporter"
                     /protein_id="HBI9793968.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIA"
     gene            1106..1181
                     /locus_tag="LAF92_005097"
     tRNA            1106..1181
                     /locus_tag="LAF92_005097"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1139..1141,aa:Asn,seq:gtt)
     gene            complement(1219..2169)
                     /gene="cbl"
                     /locus_tag="LAF92_005098"
     CDS             complement(1219..2169)
                     /gene="cbl"
                     /locus_tag="LAF92_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBI9793969.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIAALLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMESSEEEIDYQI"
     gene            complement(2271..>3102)
                     /gene="nac"
                     /locus_tag="LAF92_005099"
     CDS             complement(2271..>3102)
                     /gene="nac"
                     /locus_tag="LAF92_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBI9793970.1"
                     /translation="PALSQQVATLEGELNQQLLIRTKRGVTPTDAGKILYTHARAILR
                     QCEQAQLAVHNVGQALSGQVSIGFAPGTAASSITMPLLQAVRAEFPEIVIYLHENSGA
                     VLNEKLINHQLDMAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQSVDVNAIAQMNL
                     FLPSDYSAIRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPESAARSLC
                     GAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS
                     "
BASE COUNT          887 a          806 c          669 g          740 t
ORIGIN      
        1 cgcaattgaa aacgcgataa aatttccggc aataacactg gtccccatcc cggcaacgaa
       61 catttgggtt aataaccgac cactggtaaa taacactgat tcgacactcg cgggaatacc
      121 aatccccatg acttcccaga taatgctaaa attcagcggt ttaaaatagc tctttaacga
      181 aatccttagc gcaggattaa aaccaatcgc cagcacccac aaaattgcaa ctgcgccaat
      241 ataacgagaa atggttaaac ccagccctgc cccgacaaat cccagtcccg gccaggagaa
      301 aaggccgtaa atcaatatgc cgctaataat aatattaaga atattcaggc taccgttaat
      361 caatagcggt attttcgtat tccctgcacc acgaagtgcc ccgctaccaa taagagtgat
      421 ggcagctgct ggataactga gtaccgtcag ctccagataa gtcaacgcca gtgctttaac
      481 ttctgtcgtg gcatcacccg cgacgaaatc aataatttgt tcgccaaaat gatgaataag
      541 cgttgccaac agtacggcaa acaacgtcat gatcaccaat gactgccgcg tcgccaccct
      601 cgctcgtcgt cgatcccgct taccgagact aaatgccaca acgacagtag taccaagatc
      661 gatagcagca aaaaaagcca taatgaccat attgaagctg tccgccaatc ccacgccggc
      721 catcgcatct tttcccagcc agctgaccag aaaagtgctc agaaccccca tcaacaggac
      781 acaggcattc tccatgaaga taggaacagc aagcggggtt atctcgcgcc agaacaacac
      841 tttgtagctc ttgcgtttag cgtgccagcg agtgccgtga acaacctggc gtaaagcaga
      901 ggagatattc aaagccgacc ttaattgcag aaagtgaaac cacatttcaa ataatgaggg
      961 agaatcagca aagctgcaaa gattttcgcc aacaaattgt ctgcaaatgc aacaaactgt
     1021 tgatagaaac ggcaaacagt tggggaattt aaaaatcggg tttgacaaaa gatttttcgc
     1081 cgttaagatg tgcctcaaca acgattcctc tgtagttcag tcggtagaac ggcggactgt
     1141 taatccgtat gtcactggtt cgagtccagt cagaggagcc aaattcctga aaagcccgct
     1201 tttatagcgg gatttttgct atatctgata atcaatttcc tcttcactgc tttccatcac
     1261 ctgccgcttg atatcctcaa ctgacagtcc tgcattacaa agttccagaa agcgccagac
     1321 atagttacgc tgaagttgtc ctcgcttcag tcccaaccag acagtattag catcaaaaag
     1381 atgccgcgta tccaggcgga ttaaattctc ttcctcttgt tcgccactgg attgctcggc
     1441 aactaatccg atcccaagcc caagagcaac ataggtttta atgacatcag aatcctgcgc
     1501 acttaataca atatctgcca gcaaaccttt gcgggcaaat gcgtcatcaa tacgtgagcg
     1561 ccccgtaatc ccctgtcggt aagtgattaa cggccacttc gctattgatt ccagcgtcaa
     1621 tggtgaaatt tgcgtcaagg gatgatcgtg tggaacaagc aaactatggt gccaacgaaa
     1681 ccacgggaag gcgacgagct gcgggtcatt actcaaacgc tcgctggcga taccaatatc
     1741 agcttcgcca ttttgcaaca atgccgcaat ttcctgtggc gtcccctgga ttagctcgag
     1801 ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc tctggcaagc tataacgtgc
     1861 ctgagtatgc gtcgttgcaa tagtgagaac gccagacgta tcgttggtaa acaggtctgc
     1921 aagccgacga acattactgg cttcattcag aatacgttct gcaatgacca gtaatgcttt
     1981 gcccggttca gtcatgccca gcagtcgctt acctcgtcga acaaatattt cgatgccaag
     2041 ttcatcctcc agttcccgaa tatgacggct gacgcctgac tgtgaggtaa aaagcatatt
     2101 cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg cggattatct ttagttgttg
     2161 gaaattcacg gtaaactccg ggcagttcag atttcccgtt attgttaaag tctaatgccc
     2221 ggcataacaa ataataaaaa cccgcatctt attccatccc gatataacac ttagctcacc
     2281 aattgccact gccttttttc catcactgga gaactaatca ctgacattaa caactctttc
     2341 actgcctgtg cctgtggcga taagttcgct ctggcgggta aatttaatga caaagagaga
     2401 ctcatggaag gagtggtaat gcgtgacatc cacccattta ctgcgccaca taacgaacgc
     2461 gcggccgatt cgggtaatac tgcaacgccc atgccgctgg caatcgctgc ggtaagcgtg
     2521 gcaatagact caatttcacc aataactttt gccgtgagtc gccgtaggga aaaagcctca
     2581 tcaacacgaa gtctaatagc actgtaatca ctggggagaa agaggttcat ttgcgcaata
     2641 gcattcacat caacgctttg ccccgggcaa tcttgagttc ctaccagaaa aagatcttct
     2701 ttcagcaaag cctgactgga tacaccagcc acaggggaat gctcataaat caccgccata
     2761 tcgagttggt gatttatcaa tttttcgtta agcactgcac cactattttc atgaagatag
     2821 ataacgatct ccggaaattc agcgcgaacc gcctgtaata agggcatggt gatggatgac
     2881 gcagcggttc ctggtgcaaa gccaatcgag acttgccccg ataatgcctg accaacgtta
     2941 tgcaccgcca gttgggcctg ttcacactga cgtaaaatgg cccgcgcatg ggtatagaga
     3001 atttttccgg cgtctgttgg tgtaacgccc cgctttgtac ggatcaaaag ttgttgattt
     3061 aactcacctt ccagtgtggc aacctgctgg ctgagcgctg gt
//
LOCUS       DAEVZA010000272         3061 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEVZA010000272 DAEVZA010000000
VERSION     DAEVZA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.272"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..2493
                     /gene="traG"
                     /locus_tag="LAF92_005100"
     CDS             <1..2493
                     /gene="traG"
                     /locus_tag="LAF92_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBI9793971.1"
                     /translation="MVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIAN
                     LFQDYVQNCVMGDIYLNHKYTLEELIASADPYTLIFSRPSPLRGVYDSNNNFVTCKDA
                     SVSLKDKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQI
                     IRQNVTINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQ
                     TILTGIAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQ
                     NGAPVVLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAI
                     SPTASAAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGN
                     MVMDASGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQF
                     GSNRGSSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTSTRL
                     SGGMYADAHAEWGVKPKILGVGGGVGIRGGFKGSLDATDDDSHSASSGSRATHDARHD
                     IDAKASKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMT
                     RSHEYAEMASRTESMSGQMSEDLSQQFALYVMKHAPQDAEAILTNTSSPEIAERRRAM
                     AWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDS
                     NIRNDVKHQVDNMVTEYKGNIGDTQNSIHGEENIVDRQYSELKNNHKQEEIQQNNRYN
                     EENKRQKLMPTPSEDALKQMVDDKKERLKGPL"
     gene            2490..2999
                     /gene="traS"
                     /locus_tag="LAF92_005101"
     CDS             2490..2999
                     /gene="traS"
                     /locus_tag="LAF92_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HBI9793972.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWVLL
                     WPTFLRLVSVKSDVRDVGFDICASGMMGFLLFVAITNGMMLYLAIPDSFRKDSKIINF
                     MYSKSKTYILLFLIVFSMVSFMHSILYVFALMITFILFFLVYTIDINRYNLSAIASVI
                     GLFKKESVS"
BASE COUNT          848 a          649 c          791 g          773 t
ORIGIN      
        1 atggtggcca gttacgagat gatcttcacg caaccggaca gtgtcaccta cagcaaaacg
       61 gggatgctgt ttggcgcaga actggtatca aaaagcaccg actttttgtc ccgcaatccg
      121 gaaatcgcca atcttttcca ggattatgtg cagaactgcg tgatggggga tatttacctg
      181 aaccacaaat acacgctgga ggagctgata gcctccgctg atccctacac gctgattttt
      241 tcccgcccca gtccgctacg gggcgtttat gacagcaata ataatttcgt gacctgtaag
      301 gacgcgtcgg tttcgctgaa agacaagctg aatctggata cacagagtgg cggaaaaacc
      361 tggcattatt atgcccagca gctatttggt ggacgccctg atccgaacct gttgttcagt
      421 acgctgattg gcgacagtta cagttacttc tatggctcaa gtaaatcagc cagccagatc
      481 atccgccaga atgtcaccat caatgcgctg aaggagggga tcaccagtta tgccgcgcgt
      541 aatggtgatt cagccagcct ggtgaatctg gccaccacgt catcgatgga gaagcaacgt
      601 ctggcacatg tctccatcgg tcatgtggcg atgcggacac tgcccatgac gcagaccatc
      661 ctgacgggga tcgccatcgg tattttcccg cttctggtac tggcggctgt cttcaacaaa
      721 ctgacgctgt ccgtcctgaa aggctatgtg tttgccctga tgtggttgca gtcatggcca
      781 atgttgtacg ccattctgaa cagcgccatg acattttatg cgaagcagaa tggtgcaccg
      841 gtggtgctct cggaaatttc acagatacag ctgaaatact ccgatctggc ttccactgcc
      901 gggtatcttt ccatgatgat cccgccatta tcctggatga tggtcagggg gctcggggca
      961 ggattttcca gtgtgtacag ccatttcgct tcctctgcta tcagcccgac cgccagtgca
     1021 gcaggcagtg tggttgacgg taattactcc tacggcaaca tgcagacgga gaacgttaac
     1081 ggcttcagct ggagcaccaa cagcaccacg tcgtttggtc agatgatgta ccagaccggc
     1141 agtggcgcaa ccgccacaca gacccgtgac ggtaatatgg tgatggatgc aagcggagcg
     1201 atgtcccggt taccggtcgg catcaatgca acgcgtcaga ttgcggcggc acaacaggaa
     1261 atggcccggg aagcatcgaa cagagcagaa agtgccctgc atgggttcag cagcagtatt
     1321 gccagtgcct ggaacacgct cagtcagttt ggttctaacc gggggagcag tgattctgtc
     1381 accggtggtg ctgacagcac gatgagtgct caggactcca tgatggccag ccgtatgcgc
     1441 agtgcagtgg aaagctatgc gaaggcgcat aatatcagta atgagcaggc gactcaggaa
     1501 ctggcatcaa caagtaccag gctatctggt ggaatgtatg ctgatgctca tgctgaatgg
     1561 ggggttaaac ctaaaattct tggtgttggt ggtggggtag gcatcagagg tggatttaag
     1621 gggagcctgg atgcaacgga tgatgactca cattcagcaa gtagtggcag cagggctacc
     1681 catgatgctc gccatgatat cgatgctaaa gccagtaagg actttaaaga ggccagcgat
     1741 tactttacca gtcgcaaagt cagtgaatcc ggcagccata ctgacaataa tgccgattcc
     1801 cgcgtggacc agttgtctgc agccctgaac tcagcgaaac agagttacga ccagtacacg
     1861 acgaatatga cccgcagcca tgaatatgct gaaatggcat cccgtactga aagcatgagt
     1921 gggcagatga gcgaagattt gtcgcaacag tttgcactgt atgtgatgaa acacgcaccg
     1981 caggatgccg aagctattct gacaaacacc agttcaccgg agattgcaga acgccgtcgg
     2041 gcgatggcat ggtcttttgt tcaggaacag gtgcagcctg gtgttgataa tgcctggcgt
     2101 gaatcccgtg gggatattgg taaaggaatg gagagcgtac cttcgggtgg tggcagccag
     2161 gatattattg ctgaccatca ggggcatcag gccattattg atcaaagaac gcaggacagt
     2221 aatatccgta atgatgtaaa acatcaggtt gataatatgg tcacagaata taaagggaat
     2281 atcggagata ctcagaatag tattcatggc gaagaaaata ttgtagacag acaatattca
     2341 gagttaaaaa ataatcacaa acaggaagaa attcaacaaa acaacagata taatgaagaa
     2401 aataaacgac agaaattaat gccaacaccg tctgaagatg cattaaaaca gatggtggat
     2461 gataagaaag aacgattgaa ggggccatta tgatcactca ctatgatata aaaatggaga
     2521 tgcaaaaact aaaggaggta ctctctgtag agggtgttaa tatcccatca ttattacaag
     2581 tcataaagcc gggaacttat gtgtttttat gggttttatt atggccaact tttctgcgtt
     2641 tagtatcggt taaaagtgat gtcagggatg ttggctttga tatatgtgct agtggaatga
     2701 tggggttcct gctttttgtg gctattacaa atggaatgat gttatacctt gcgattcctg
     2761 atagtttcag aaaggattca aaaataataa attttatgta ttctaagagt aagacatata
     2821 ttttgttgtt tttgattgtt ttttctatgg tttcgtttat gcattctatc ttatatgttt
     2881 ttgcactaat gattacattc atattatttt tcttggtgta taccatagat ataaatcgtt
     2941 ataatctctc agctatagcc tcagtaatag gactgttcaa aaaagagtct gttagttaaa
     3001 aggatatttt ggatgaaaac aaaaaaattg atgatggttg cactggtcag ttccactctg
     3061 g
//
LOCUS       DAEVZA010000273         3041 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEVZA010000273 DAEVZA010000000
VERSION     DAEVZA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.273"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..424)
                     /gene="flhC"
                     /locus_tag="LAF92_005102"
     CDS             complement(<1..424)
                     /gene="flhC"
                     /locus_tag="LAF92_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBI9793973.1"
                     /translation="MSEKSIVQEARDIHLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCG"
     gene            complement(427..777)
                     /gene="flhD"
                     /locus_tag="LAF92_005103"
     CDS             complement(427..777)
                     /gene="flhD"
                     /locus_tag="LAF92_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBI9793974.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            1557..1985
                     /gene="uspC"
                     /locus_tag="LAF92_005104"
     CDS             1557..1985
                     /gene="uspC"
                     /locus_tag="LAF92_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBI9793975.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1992..>3041)
                     /gene="otsA"
                     /locus_tag="LAF92_005105"
     CDS             complement(1992..>3041)
                     /gene="otsA"
                     /locus_tag="LAF92_005105"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBI9793976.1"
                     /translation="IIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPT
                     YDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIG
                     IEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYP
                     QHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRK
                     LLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIV
                     NPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSA
                     ESQQRDKVATFPKLA"
BASE COUNT          843 a          682 c          666 g          850 t
ORIGIN      
        1 cgccgcagca gttgcagctg gaaagttgca gtaatccact ttcaacaaac cgcaccaatg
       61 tccaggcacg ggttaatgcc agcagtggtc cttcttctgc ttgtgggcac tgttcaaggt
      121 ataaacggta ggctttgatc accgcatcga cgccattaca caaaccggtt ttcagtaaaa
      181 actgccatgc attacagaac atcgaagcat gaacgttttg ttcccaggtc ataaaccagt
      241 cggttgagaa tggcagcatg cctttcggcg gtgggcttcc gcgcagttct ttataaagtt
      301 ttatcaggcg tccgcgactt aactgtgttt cgctttccag catctgcaaa cgagctccca
      361 gggtgatcaa ttccattgcc agatgaatat cccgcgcttc ctgaacaatg cttttttcac
      421 tcatgatcag gcccttttct tgcgcagcgc ttcttcaggc tgattaacat cattcagcaa
      481 gcgtgttgag agcatgatgc cggtatgaat ttgctggaga tcgtcaacgc gggaatcttg
      541 cgtcaactga gtaatcgtct ggtggctgtc aaaacggaag tgacaaacca gttgattggt
      601 ttctgccagc ttaaccattt gcggaagagt cagtgccgct aacgttgtcg ccatttcttc
      661 atttatgccg agacgaaaca tagcggacgc tttgtcctga acaatcaaac gctgtgcaag
      721 tagtaaatat gacaagttga tgtcataaat gtgtttcagc aactcggagg tatgcattat
      781 tcccacccag aataaccaac tttattttta tgcggtttca ccgcaccccg tgatgtcgcc
      841 gggaagcccc ggtaaaaaat aattagcatt agaatagttg cgataagctg caataagcag
      901 aaccaccttt ttggtttaat atgtccttac aaatagaaat gggtctttac acttatctaa
      961 gatttttcct aaatcgacgc aactgtactc gtcactacac gcacatacaa cggagggggg
     1021 ctgcgatttt caataatgcg tgatgcagat cacacaaaac actcaattac ttaacataaa
     1081 tgtataagtc atacttttgt tttgggtgta tttcaatctg ttaaaaagtt tttcgctacg
     1141 ctagcaagca aaaatgaaac aggaataatc gaaatgggat gttgcgcaca gttaaaataa
     1201 ctcaccgtaa ataatcatct gctataaata atcactttca tgcaatacca gataagctat
     1261 ttttaaacag acacttaccg cacaacaaac taattaaata caaagaaagg caacaatcaa
     1321 cttaatgtta atgaaatgtt ttacatatat taaccacttg tttaatgcgg agaatcagta
     1381 cactattttc ttataattac atttgaaatt atatgatcac cgggagtgat tgattaattg
     1441 tctttactaa taatcagact aatatttacc tgtttgaccg agttgggatt gcgtcgtttc
     1501 tccattagga gtaaagcttt aatgtcacct gaagttcaca gaataaggaa caggttatga
     1561 gctatagcaa tattcttgtc gctgttgcgg ttacaccgga aagtcagcaa ctgctggcaa
     1621 aagcggtatc tatcgccagg ccagtaaagg gacacatcag tttaattact ctcgcttccg
     1681 acccggaaat gtacaatcaa ttagccgcgc cgatgcttga agatttacgc agcgtgatgc
     1741 aagaagaaac gcaaagcttt cttgataagt taattcagga tgcggggtat cccgttgaca
     1801 agacgtttat tgcctacggt gagttaagcg aacatattct ggaagtatgt cacaaacatc
     1861 atttcgattt ggtgatttgc ggtaatcaca atcacagttt cttttcgcga gcatcctgct
     1921 cggcgaaaag agtgattgcc tcaagtgagg tcgatgtgct gttagttcca cttacgggtg
     1981 attaaccgct cctacgcaag ctttggaaag gtagcaactt tatcgcgctg ctggctttcc
     2041 gcgcttcgcg gaactatctg ctttaggtcg ctaatgaagc actcctgcca gtggttaata
     2101 tcgtttttca cgataacgtc cagcatttct gcatgacggg aaatacgttc cgccagcgac
     2161 atagtcaatg cacgatccag cgcagctgca acttcgtcac gatcgtaggg gttaacaatt
     2221 aacgccgacg ttaactcgtt tgccgctccc gcaaattgcg aaagaacaag aacgcccggg
     2281 ttggctgggt cctgagcagc aacatactct tttgctacca ggttcatccc gtcacgcagt
     2341 ggcgtcacta agcccacgtc agagtagcgg aatattttca tcagtaattt acggtcaaaa
     2401 tgctgattca aataataaag cggcgtccag cctaattgcc cgtatttacc attaattcgt
     2461 ccagcttcat tttcgagctg atgacgaata tcctgatagg cttgcacatc accacgcgac
     2521 gttggtgcaa tctgggtata acgaatttta ccatgatgct gcggatattt ttccagcaac
     2581 gcttcatagg cgagaaaacg ctctggcaaa cctttggaat aatccagccg ttcgacagaa
     2641 aagatatttt gtacgttttt cagttccgct ttaagttgcg ccagttttgg cggcagtggc
     2701 ccggcagcct gtttggctat ttctttcggt tcaatgccga tcgggtagac ttctgttcga
     2761 aatgctttgc cccaggctgt atggcttttt gcgctacgtg tcgtgacgcg ggtcaggtta
     2821 gaaagacaat ccaggaacgc cagacgatcg ttttctgtct ggaaacccag caaatcataa
     2881 tcacaaagct gttcaagcaa ggtgtcatat gtcggcagcg cgttgaagat ttccggtgtc
     2941 gggaaaggaa tatgcagaaa gaaaccaatg cgattattca ctccccgttt gcgtaattca
     3001 tgcgcaaatg gcaacaggtg ataatcgtgg atccagataa t
//
LOCUS       DAEVZA010000274         2947 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEVZA010000274 DAEVZA010000000
VERSION     DAEVZA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.274"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..800
                     /locus_tag="LAF92_005106"
     CDS             <1..800
                     /locus_tag="LAF92_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI9793977.1"
                     /translation="GDPDQPIIMGRTYHHENRTPGSLPGTKTQMTIRSKTYKGSGFNE
                     LKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQTVGV
                     NQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHK
                     SLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIF
                     LNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            867..>2947
                     /gene="rhsD"
                     /locus_tag="LAF92_005107"
     CDS             867..>2947
                     /gene="rhsD"
                     /locus_tag="LAF92_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HBI9793978.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGLWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSE"
BASE COUNT          671 a          807 c          972 g          497 t
ORIGIN      
        1 acggcgaccc ggaccagccg attattatgg ggcgtaccta ccaccatgaa aaccgcacac
       61 ccggcagcct gccggggacg aagacgcaga tgacgatccg ctcgaaaacc tataagggca
      121 gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc tacatccacg
      181 cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg ataaacaacc
      241 atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag atacagacgg
      301 tgggcgttaa ccagatagag acggtgggca gtaaccagat cattaacgtg ggttctgttc
      361 aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac cagacgacgg
      421 taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc ggtttgcata
      481 aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt accttcaccg
      541 ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc ggtgagcatc
      601 tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa atttttctca
      661 acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca ctgttgatta
      721 actggaactg tgcagcctcg aaatccccac cgaagacccc tgatgaaaag caggatacgc
      781 cggatatgag agagtactga tcccgtacct gttcggtatt cagtaacaaa caagcattaa
      841 aaataaacag aagaaggaat tgaatcatga gcggaaaacc agcggcgcgt cagggagata
      901 tgactcagta tggcggtcct attgtccagg gttcggcagg tgtaagaatt ggcgcgccca
      961 ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg gtaaatccgc
     1021 tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc ccgctgccgt
     1081 tcattctctc ccgcacctac agcagctacc ggaccaggac acccgcgccg gtgggagtgt
     1141 ttggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgcgat gatgcactgg
     1201 tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg ggggaggcgg
     1261 tgtacagccg cagcgagtca atgtggctgg tgcgcggtgg taaggcagcg cagccggacg
     1321 gccacacgct ggcgcggctg tggggggcgc tgccgccgga tatccggtta agcccgcatc
     1381 tttacctggc gaccaacagc gcacaggggc tgtggtggat actggggtgg tcagagcggg
     1441 tgccgggtgc tgaggacgta ctgccagcgc cgctgccgcc gtaccgggtg cttaccgggc
     1501 tggcagaccg tttcgggcgg acgctgacgt accggcgtga ggccgcaggc gacctggccg
     1561 gggaaatcac cggcgtgacg gacggtgccg ggcgtgagtt ccgtctggta ctgaccacac
     1621 aggcacagcg ggcggaagag gcccgcacct cttcgctatc ttcttctgac agttcccgcc
     1681 ctctctcagc ctcaccgttc cccgacacac tgcccggtac cgaatacggc cccgacaggg
     1741 gcatccgtct ttcggcggtg tggctgatgc acgacccggc atacccggaa agcctgcccg
     1801 gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg gtatatgacc
     1861 gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc cggatggtgg
     1921 cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacc gggcgggtgg
     1981 tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag gaccgcatca
     2041 ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg gccgggctga
     2101 agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg tatgacgcgg
     2161 caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac ggtctgaatg
     2221 tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg aaattttact
     2281 ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag agccgccggg
     2341 aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag acagtacgct
     2401 accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg acgggcagca
     2461 cccggcagat gacgtggagc cgctacgggc agttgctggc gttcactgac tgctcgggct
     2521 accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggttcac cgcgaggaag
     2581 gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg aaagacgcac
     2641 agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc gttatcaccc
     2701 cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc agcaccacgc
     2761 agggcgggct gacgcgcagc atggaatacg atgctgccgg acgtgtcatc agtctgacta
     2821 acgagaacgg cagtcacagc gacttcagct acgatgcgct ggaccggttg gtacagcagg
     2881 gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa ctcacgcaga
     2941 gtgaaga
//
LOCUS       DAEVZA010000275         2929 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEVZA010000275 DAEVZA010000000
VERSION     DAEVZA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.276"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1089
                     /locus_tag="LAF92_005108"
     CDS             <1..1089
                     /locus_tag="LAF92_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1974 domain-containing protein"
                     /protein_id="HBI9793979.1"
                     /translation="YHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITV
                     EGANILTRSMMIFGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSF
                     WLGLTRGLTSSTPTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLG
                     DILSQLYLASAVLKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAG
                     LLNVVIFPTGRHYLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEA
                     LVDVIAADPIHQRICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRS
                     INVDDFDPEELATKPVKLPEKVRKVEAA"
     gene            complement(1132..1578)
                     /gene="ivy"
                     /locus_tag="LAF92_005109"
     CDS             complement(1132..1578)
                     /gene="ivy"
                     /locus_tag="LAF92_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBI9793980.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            1759..2529
                     /gene="yafV"
                     /locus_tag="LAF92_005110"
     CDS             1759..2529
                     /gene="yafV"
                     /locus_tag="LAF92_005110"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBI9793981.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(2571..>2929)
                     /locus_tag="LAF92_005111"
     CDS             complement(2571..>2929)
                     /locus_tag="LAF92_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBI9793982.1"
                     /translation="CVAVSFRSIIAEQKKEPEMTVRYYISSADLTVEKFATAIRNHWH
                     VENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAA
                     MDRNYLASVLAESGLS"
BASE COUNT          665 a          736 c          790 g          738 t
ORIGIN      
        1 tatcactgta cccaccgcgg gcagcagtcg attattgatg cgatggatat cactggtggc
       61 aaaggcatta tgctcgggca aagcaacttc ctggcgcgtg cttaccaggg cgcaccgatt
      121 gccatcaccg ttgaaggggc taacattctg acccgcagca tgatgatctt cggacaagga
      181 gcgattcgtt gccatccgta cgtgctggaa gagatggaag cggcgaagaa caatgacgtc
      241 aacgcgttcg ataaactgtt gttcaaacat atcggtcacg tcggtagcaa caaagttcgc
      301 agcttctggc tgggcctgac gcgcggttta accagcagca cgccaaccgg cgatgccact
      361 aaacgctact atcagcacct gaaccgcctg agcgccaacc tcgccctgct ttctgatgtc
      421 tcgatggcag tgctgggcgg cagcctgaaa cgtcgcgagc gcatctcggc ccgtctgggg
      481 gatattttaa gccagctcta cctcgcctct gccgtgctga agcgttatga cgacgaaggc
      541 cgtaatgaag ccgacctgcc gctggtgcac tggggcgtac aagatgcgct gtatcaggct
      601 gaacaggcga tggatgattt actgcaaaac ttcccgaacc gcgtggttgc cgggctgctg
      661 aatgtggtga tcttcccgac cggacgtcat tatctggcac cttctgacaa gctggatcat
      721 aaagtggcga agattttaca agtgccgaac gccacccgtt cccgcattgg tcgcggtcag
      781 tacctgacgc cgagcgagca taatccggtt ggcttgctgg aagaggcgct ggtggatgtg
      841 attgccgccg acccaattca tcagcggatc tgtaaagagc tgggtaaaaa cctgccgttt
      901 acccgtctgg atgaactggc gcacaacgcg ctggcgaagg ggctgattga taaagatgaa
      961 gccgctattc tggtgaaagc tgaagaaagc cgtctgcgca gtattaacgt tgatgacttt
     1021 gatccggaag agctggcgac gaagccggta aagttgccgg agaaagtgcg gaaagttgaa
     1081 gccgcgtaaa tgaataacgg agccgaaagg ctccgtttct ttatccgcta attatttaaa
     1141 attaaagcca tccggatggt tttccaggct gccggtcaac gccgcgaaca acaccgtttt
     1201 accatcaatc gaaagcgcat cgttcacatt cagccaggtg agtttctctt gcgacgtttt
     1261 ctcatcaata gtcgagaaca gccccgtcat ctgattagat ttctcggacc acatcacagc
     1321 gatacgttgc gagccacagt catgcggttt gcacgcgctc atcacctgat acgtctcatc
     1381 tcccaacgtt acggtttgtg cgggagtata agtaccgcct ttcatcaccc aggcaggcag
     1441 cttatgccct tgtaccatct gattaaatgc agctttggtg gtttcgccct ttgcaaggct
     1501 gctaatggtt aaatcatcct gcgccattgc actggtggcg atgaccagag cggcgactgt
     1561 cgttattgcc ttaaacatca ttcctcccga gcttatcctg cccatgttat taaccttcta
     1621 aagcctggcg ataacacgat aatttgcaaa tcagatattt ccaatatcag catttgttat
     1681 caatttattc tgcgctaagg caactgcctg ataaggtgtt aaagttataa aacccttcgc
     1741 ataaaggagt tgtacaccgt gcctggtttg aagattacgc ttttgcagca accactggtg
     1801 tggatggatg gccctgccaa cctgcgtcat tttgatcgtc aactggaagg tattaccggg
     1861 cgcgatgtga tcgttctacc ggagatgttt accagcggct ttgccatgga agcggcagct
     1921 tcgtcgctag cacaagatga cgtagtgaac tggatgacag ctaaggcgca gcagtgcaat
     1981 gcgctgattg caggcagtgt tgcattacaa acggagtctg gttcggttaa ccgctttttg
     2041 ctggttgagc cgggcggcac ggtacatttt tatgataagc gtcatctgtt ccgcatggca
     2101 gatgagcatc tacattataa agcgggcaat gcgcgagtga ttgtggaatg gcgcggctgg
     2161 cgtattttgc cgttggtgtg ctacgactta cgttttccgg tgtggtcgcg caatctcaac
     2221 gattatgacc tcgccatcta cgttgccaac tggcctgctc cgcgctctct gcactggcag
     2281 gcattgctga cggcccgcgc gattgagaat caggcgtatg tggcgggatg caatcgcgtc
     2341 ggcagcgatg gcaacggctg ccattatcgc ggtgacagcc gggtgattaa cccgcagggc
     2401 gagattatcg ctactgccga cgcgcatcag gcaacgcgca ttgatgcgga gctgtcgatg
     2461 gtggcattgc gggaatatag agaaaagttt ccggcatggc aggatgcgga tgagtttagg
     2521 ttgcggtgag tgggggttgt gtttaaggct gggggagagt cggggtaaga ctacgaaagc
     2581 ccgctctccg caaggactga cgccagatag tttctgtcca tagctgcttt tcgcatctta
     2641 cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa tattaatagc gatgtgccgt
     2701 atccctgaaa ataattctgc ggcgtttcct cttcttattt tgcagtcgtc ttcattcatt
     2761 accacgtcca gacgccagtg cagcttattc tccacgtgcc agtggtttcg gattgctgtg
     2821 gcgaactttt ctacggttaa atcagcagaa ctgatataat atctgaccgt catttctggc
     2881 tctttctttt gttctgctat tattgaccga aaggagactg ccacgcata
//
LOCUS       DAEVZA010000276         2907 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEVZA010000276 DAEVZA010000000
VERSION     DAEVZA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.277"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..468
                     /locus_tag="LAF92_005112"
     CDS             <1..468
                     /locus_tag="LAF92_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBI9793983.1"
                     /translation="SGKHGPFLGCSQYPACDYVRPLKSSADGHIVKVLEGQVCLACGA
                     NLVLRQGRFGMFIGCSNYPECEHTELIDKPDETAITCPQCRTGHLVQRRSRYGKTFHS
                     CDRYPECQFAINFKPIAGECPECHYPLLIEKKTAQGVKHFCASKQCGKPVSAE"
     gene            473..1045
                     /gene="tsaC"
                     /locus_tag="LAF92_005113"
     CDS             473..1045
                     /gene="tsaC"
                     /locus_tag="LAF92_005113"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBI9793984.1"
                     /translation="MNNNLQGDAIAAAIDVLNKERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            1050..1868
                     /gene="aroE"
                     /locus_tag="LAF92_005114"
     CDS             1050..1868
                     /gene="aroE"
                     /locus_tag="LAF92_005114"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBI9793985.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            1865..2122
                     /locus_tag="LAF92_005115"
     CDS             1865..2122
                     /locus_tag="LAF92_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBI9793986.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(2098..2652)
                     /locus_tag="LAF92_005116"
     CDS             complement(2098..2652)
                     /locus_tag="LAF92_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBI9793987.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT          748 a          685 c          727 g          747 t
ORIGIN      
        1 tccgggaaac acggtccgtt tcttggatgc tcacagtatc cggcgtgtga ctacgtccgt
       61 cctctgaaat cttcagcgga tggacatatt gtcaaagttc tggaggggca ggtttgcctt
      121 gcatgtggcg caaatctggt attacgccag ggacgctttg gtatgtttat tggttgcagt
      181 aactaccctg aatgcgaaca taccgaactt atcgataaac cggacgaaac agcaattaca
      241 tgcccccaat gccggacggg ccatctggtc cagcgccgct cccgttatgg caaaacattt
      301 cactcctgtg atcgctaccc ggagtgtcaa tttgccatta acttcaaacc catagctgga
      361 gaatgccctg agtgtcatta tccactactc atcgaaaaga aaaccgcgca gggtgtaaaa
      421 cacttttgtg ccagtaaaca atgtggaaag ccggtttcgg cggaataata acgtgaataa
      481 taacctgcaa ggagacgcta tcgcagctgc gatagatgtc ctcaataaag aacgtgtcat
      541 cgcctatcca acggaagccg ttttcggtgt tgggtgcgat cctgatagcg aaacagcagt
      601 gatgagactg ttggagttaa aacagcgtcc ggttgataag gggctgattt taatcgcggc
      661 aaattacgag cagcttaaac cctatattga tgacaccatg ctgactgatg cgcagcgtga
      721 aaccattttt tcccgctggc caggtcctgt cacctttgtc tttcccgcgc ctgcgacaac
      781 accgcgctgg ttgacgggcc gctttgattc gcttgctgta cgagtcaccg accatccgtt
      841 ggtggttgct ttgtgccagg cttatggtaa accgctggtt tctaccagtg ccaacttgag
      901 cggattgcca ccttgtcgaa cagtagacga agttcgcgca caatttggcg cggcgttccc
      961 ggttgtgcct ggtgaaacgg gggggcgttt aaatccttca gaaatccgcg atgccctgac
     1021 gggtgaactg tttcgacagg ggtaacataa tggaaaccta tgctgttttt ggtaatccga
     1081 tagcccacag caaatcgcca ttcattcatc agcaatttgc tcagcaactg aatattgaac
     1141 atccctatgg gcgcgtgttg gcacccatca atgatttcat caacacactg aacgctttct
     1201 ttcgtgctgg tggtaaaggt gcgaatgtga cggtgccttt taaagaagag gcttttgcca
     1261 gagcggatga gcttactgaa cgggcagcgt tggctggtgc tgttaatacc ctcaagcggt
     1321 tagaagatgg acgcctgctg ggtgacaata ccgatggtat aggtttgtta agcgatctgg
     1381 aacgtctgtc ttttatccgt cctggtttac gtattctgct catcggcgct ggtggagcat
     1441 ctcgcggcgt actactgcca cttctttccc tggactgtgc ggtgacaatc actaatcgga
     1501 cggtatcccg cgcggaagag ttggctaaat tgtttgcgca cactggcagt attcaggcgt
     1561 tgggtatgga cgaactggaa ggtcatgagt ttgatctcat tattaatgca acatccagtg
     1621 gcatcagtgg tgatattccg gcgatcccgt catcgctcat tcatccaggc atttattgct
     1681 atgacatgtt ctatcagaaa ggaaaaactc cttttctggc atggtgtgag cagcgaggct
     1741 caaagcgtaa tgctgatggt ttaggaatgc tggtggcaca ggcggctcat gcctttcttc
     1801 tctggcacgg tgttctgcct gacgtagaac cagttataaa gcaattgcag gaggaattgt
     1861 ccgcgtgaat caggccatcc agtttccgga cagagaagag tgggacgaga ataaaaaatg
     1921 tgtatgtttt cccgctctcg tgaatggcat gcaactgaca tgtgcgatct ctggcgagag
     1981 tctggcgtat cgttttactg gagatacgcc agaacagtgg ttagcgagtt ttcgtcagca
     2041 tcgctgggat ctggaagaag aagcggaaaa cttaattcag gaacaaagtg aagatgatca
     2101 aggctgggtc tggttaccct gatccagata ttcgtccttc catttcacgt aattattcgc
     2161 ggaatagcgt aacccagcct tctcttcatc acttaacggg cggatctgtt tgacggggct
     2221 accgagatac agatatccgc tctccagccg tttattttgt gggaccagac tacccgcacc
     2281 aatcatcaca tcatcttcta ctattgcgcc atcaagtaaa attgagccca tcccaaccaa
     2341 aactcgattg ccaatggtgc agccgtggag catcaccttg tgaccaacag tgacatcttc
     2401 gccaatggtt aatgggttgc catctgggtt gtacgaggat ttatgagtga catgcaacat
     2461 actgccatcc tggatattgg tgcgtgctcc gatctgtaca taatgtacat ctccacgaat
     2521 cacaacgagc ggccagatcc ccacatcatc agccagacga acgtcaccaa tcacgacact
     2581 gctatcgtcg atcattacgc gctgaccgat ttgtggaaaa agatcgcggt atgggcgtaa
     2641 aacatcagac atacttacct cagcaataaa tgatttacta atgactttgg gggcattatt
     2701 ggccttgtgc aagtctttta gtatgcaaaa aagcaccgtt ttgtgtgcga atgcagcaaa
     2761 aagggtgaaa aaacaacaaa cagaaaaaaa gatcaaaaaa gtacttgtgc aaaaaattgg
     2821 gatccctata atgcgcctcc gttgagacga caacgtgaaa cacttcacaa gatggtcgta
     2881 acaacgaaga gaaaaaatcc tgaaatt
//
LOCUS       DAEVZA010000277         2878 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEVZA010000277 DAEVZA010000000
VERSION     DAEVZA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.278"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>2878)
                     /locus_tag="LAF92_005117"
     CDS             complement(<1..>2878)
                     /locus_tag="LAF92_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBI9793988.1"
                     /translation="SETVLGTEVKYDTPITRTITSANIDRLRFTFGVQALVETTSKGD
                     RNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQYLASVVVDNLPPRPFNIRMRRMTPD
                     STTDQLQNKTLWSSYTEIIDVKQCYPNTALVGVQVDSEQFGSQQVSRNYHLRGRILQV
                     PSNYNPQTRQYSGIWDGTFKPAYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYV
                     IGQNCDQSVPDGFGGTEPRITCNAWLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQ
                     DRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETATELVED
                     TPAILRYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDSSVGAEGLRHVPGD
                     VIEICDDDYAGISIGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPVSVEV
                     QSVTDGVKVKVSRVPDGVAGYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVP
                     EKEAIVDNGAHFDGDLSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGGS
                     FLLRLTVAADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNAWGQQGDPASVSF
                     RIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSETRITDIRQVETTARYLG
                     TGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGKITES
                     HLGKELLEKVDLTEDNASKLEQFSKEWKDANDKWNAMWAVKIEQTKDGKHYVAGIGLS
                     MEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSG
                     GNPPAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVK
                     TVSKSFPRTNSYASGTITVRISDDQKFDRQVMIPPVLFRGGKHEN"
BASE COUNT          561 a          897 c          741 g          679 t
ORIGIN      
        1 aattctcatg cttaccaccg cggaataaca ctggcggtat catgacctgc cggtcaaatt
       61 tctgatcatc actgattctt accgtgatgg tgccactggc ataactgttc gtgcggggga
      121 aagacttgct gaccgttttg acaatatcgc cttcaatctg attggctgac agtttcccct
      181 taatctgaca gttctcatta atcgtgacgt tgttgagcgt cccggagttc gcattcacac
      241 tgccactgat atccgcattt ttagcagtca gctttccgtc cggtgtcagg gaaaatgccg
      301 gtggatttcc accgctggta atggtggggg ccgtcaggcg cttcaggaac acgtcgttca
      361 tgaatatctg gttgccctgc gccacaaaca tcggcgtttc attcccgttt gccgggtcaa
      421 taaacgcgat acgattggcg gcaaccagaa actggctcag tttgccttcc tccgtgtcct
      481 ccatgctgag gccaataccc gcgacataat gtttgccgtc tttggtctgc tcaattttga
      541 cagcccacat ggcattccac ttatcgttag catccttcca ctctttcgaa aactgctcca
      601 gtttgctggc gttatcctcc gtcaggtcga ctttttccag cagctcttta ccaagatggg
      661 attcggttat cttgcctttg aaaaaatcca ggtaaccttc cgcatcatcg ctcgcccgac
      721 cgacggcctc cacgaatgcc gatttgccaa cggtgttcac actgcggata taaaagtaat
      781 aatcatggcc cggtttgata ttgatactgg cggctatcca gtacagcccc gtgccaaggt
      841 agcgggctgt ggtttcaacc tgcctgatat cggtaatccg cgtttccgag aaccagaact
      901 caaattgtac cgtcggatca taaaccgcaa gatgcggcgt ggcggttatc tgaaaatagc
      961 ccggtgtcag ctcaatccga gacggcgctg ccggtgcggc aatccggaac gataccgatg
     1021 ccggatcgcc ctgctgtccc cacgcattta ccgcccggac agtcagcctg tagttcccca
     1081 gcgccagttg cgtgaagcgg tatgtggttt ccgtcgtcct ggccgtactg accagccgct
     1141 cactgccatc gtccgctgcc acggtcaggc gaagcaggaa gctccccccc ttcaccacct
     1201 tcggcgtgtc ccagcgcgcc agcacctgat attccccgct gtctgcggtg acttcggcag
     1261 tcaggtgctg caccgcgggc ggcgtgacgc cattcaccgt gccgctcagg tcgccgtcaa
     1321 agtgcgcccc gttatccacg atggcttctt tttcgggtac atgctgcacg gcggtgatgg
     1381 catacgtgcc gtcgtcgttc tcacggatac tcacgcagcg gaacaggcgc tggcgcaacg
     1441 tcggcagctt cagcccccat acgctgtatc cggcaacgcc gtcaggaaca cggctcactt
     1501 ttaccttcac gccgtcggtg acggactgga cctccacgct gaccggattg ccacttccgt
     1561 caaccaggct tatcagcgtg gtaccggagg atggcagcgt gatttcacgg tcgagcgtca
     1621 gcgtccgggt ctggctgttc accgccagca cgcgcccgcc gatgctgatc cccgcatagt
     1681 catcatcgca gatttcaata acatcacccg gcacatggcg aagcccttcg gcacccacgc
     1741 tggaatccac ggtctgcgtc tccagcagct ccgttttaat cagccacagc ccggcgcggt
     1801 gtgcctgccc ccggctggta cagccaaagg catccatctt cgtgacgtta cgaccgtaac
     1861 ggagaatggc cggcgtgtcc tccacaagct ctgtcgccgt ctcccagccg ttatccgggt
     1921 caatccagtt cacctcaacg gcattatggc ggtccttcag ggcgctgaag ctgtagcgga
     1981 acggcgcgcc atcatccggc atcaccacat tactgcggtt ataggtccac accttatccg
     2041 acggtcggtc ctgcacgaac gtcagcgtct gcccgttcca taccggcata cagcgcatcg
     2101 ccgagcagaa atcactgagc acatcccacg ccttacgctg tgtggtcagc caggcattac
     2161 aggtgatgcg cggctccgtg ccgccaaatc cgtcaggcac cgactggtcg caattctggc
     2221 cgatgacata cagcgcccat ttatccacat ctgctgcacc aagacgtttc cccatgccgt
     2281 agcgcggatg ggtcagcata tcccacagac accaggccat gttgttgctg tatgccggtt
     2341 taaacgttcc gtcccagata ccactgtatt gccgcgtctg cgggttataa ttcgacggca
     2401 cctgcagaat acgcccgcgc agatgataat tacggctcac ctgctggctg ccgaactgct
     2461 ccgagtccac ctgcacgccg accagtgccg tgttcgggta gcactgtttc acatcgataa
     2521 tttcggtgta tgacgaccag agcgttttgt tctgcagctg gtctgtggtg ctgtccggcg
     2581 tcatcctgcg catccggata ttaaacgggc gcggcggcag gttatccacc accaccgagg
     2641 ccagatactg tgaagtggtt ttacccttaa tggtgatgtc tttttccgtc acccagccac
     2701 cgttacgttg tatctgaacc agcaggcgga cttccgatgg attcctgtcc ccctttgagg
     2761 tggtttccac cagtgcctgc acgccgaaag taaaacgcag acggtcaatg tttgccgacg
     2821 tgatggtccg ggtgatcggc gtgtcatatt tcacttctgt accgagcacc gtctcgga
//
LOCUS       DAEVZA010000278         2833 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEVZA010000278 DAEVZA010000000
VERSION     DAEVZA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.279"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..66)
                     /locus_tag="LAF92_005118"
     CDS             complement(<1..66)
                     /locus_tag="LAF92_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmF-related methyltransferase"
                     /protein_id="HBI9793989.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLAT"
     gene            342..602
                     /locus_tag="LAF92_005119"
     CDS             342..602
                     /locus_tag="LAF92_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI9793990.1"
                     /translation="MKKCLTLLIATVLSGIFLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            867..>2833
                     /locus_tag="LAF92_005120"
     CDS             867..>2833
                     /locus_tag="LAF92_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HBI9793991.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLP"
BASE COUNT          738 a          762 c          684 g          649 t
ORIGIN      
        1 cgtggcgaga tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc
       61 ggacatagca ttctccttta agcagcggcg taagataccc gcataacgcg acagggtaaa
      121 tatctggctg cgtttatcat tccccaaaac cttaacatgg ctatggttta tggcaattca
      181 tcataaaaat cagcattatt catgtcattg atttgtctta ttttgtaaaa atatatcgtt
      241 caaaaaataa gcacatctct gtcaattttt tgcgtcattt tcatcgccca ggcgacctac
      301 acttatcagg cactacccgt tgtatttatg aggatgtcat tatgaaaaag tgcctcacac
      361 tactgattgc caccgtcctg agcggaatct ttctcacggc ttatgccgcg caaccgatga
      421 gtaacctgga cagcggtcag ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa
      481 acctaagcga tctggaggat aaactggcag aaaaagcgcg cgagcaaggc gcgaaaggtt
      541 atgtcattaa ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc atctacaaat
      601 aaccccacca acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa
      661 aaaacatcaa atatgaactc aatgtaaata aatgtatttc tttttcgcgc aatgggtgat
      721 agaaaatcgc gctaagtgat aatgcttatc aaaattatta tcacttgcac gaacactatc
      781 acgggattaa cagtggcatc gcatccgcag agatgctttc tcgtggcagt gaaaatttca
      841 acatataaga aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct gccagacaat
      901 ttcattcgct cacgttcttt gccggtcttt gtattggcat cacgcctgtg gctcaggcac
      961 tcgccgccga agggcaaact aacgcggatg acacgctggt tgtcgaagca tcaacgcctt
     1021 cgctttatgc gccacaacaa tctgccgatc cgaaattctc gcgtccggta gcggatacta
     1081 cccgcacgat gacggtgatt tctgaacaag tgattaaaga tcagggcgca accaacctta
     1141 ccgacgcgct caaaaacgtc cccggcgtgg gtgcgttttt tgcgggtgag aacggtaact
     1201 ccaccactgg cgacgccatt tatatgcgcg gtgctgatac ctctaacagt atttatattg
     1261 atggcattcg cgatatcggc agcgtctcgc gcgacacctt caatactgag caggtcgaag
     1321 tgattaaagg gccttccggc accgactacg ggcgcagcgc gccgacgggc tcgatcaata
     1381 tgatcagcaa gcagccgcgc aatgattccg gcattgacgc ctccgccagt attggcagcg
     1441 cctggttccg ccgcggcacg ctggacgtca atcaggtcat tggtgatacc actgcggtgc
     1501 gcctgaatgt gatgggcgaa aaaacgcacg atgccggacg cgacaaagtc aaaaatgagc
     1561 gttacggcgt cgccccttct atcgcttttg gccttggtac agcgaatcgt ttgtatctta
     1621 attatctgca tgtcacccag cacaacacgc cagacggcgg cattccgacc atcggtttgc
     1681 cgggttattc tgccccatct gcgggaacag cggctctgaa tcattccgga aaagttgata
     1741 ctcataactt ttacggcacg gattccgatt acgacgattc gaccaccgac accgccacca
     1801 tgcgttttga gcacgacatc aacgataaca ccaccattcg caatactacc cgttggtcgc
     1861 gcgtaaagca ggattacctg atgacggcga ttatgggcgg ggcgtcgaat attactcagc
     1921 ccaccagcga tgtgaatagc tggacctggt cacgcacggc gaataccaaa gatgtgagta
     1981 ataaaattct caccaaccag accaacctga cctcgacgtt ctataccggt tctatcggtc
     2041 atgatgtcag taccggcgtg gaatttaccc gtgaaacgca gactaactac ggcgttaatc
     2101 cggtgacgtt acccgcggta aatatttatc atcctgacag cagcattcat cccggcggcc
     2161 tgacgcgcaa cggcgcaaac gccaatggtc agacggatac cttcgcaatt tacgccttcg
     2221 atacgctgca aatcacccgt gattttgagc tgaacggcgg gatccgtctg gataattatc
     2281 atactgaata tgacagtgcc accgcctgcg gcggcagcgg acgcggtgcc atcacctgcc
     2341 cagctggtgt ggcaaaaggt tctccggtca ccaccgtcga caccgccaag tcgggcaatc
     2401 tggtgaactg gaaagccggg gcgctgtatc acctgacgga aaacggtaat gtctatatta
     2461 actatgccgt ttcccagcag cctccgggcg gcaacaactt cgcccttgcg cagtctggca
     2521 gcggtaacag tgccaaccgc accgatttta aaccgcaaaa agccaacacc agcgagattg
     2581 gcaccaaatg gcaggttctg gataaacgcc tgttgctcac cgccgcgctg ttccgtactg
     2641 atatcgaaaa tgaagttgag caaaatgatg acggaactta ctcgcaatac ggtaagaaac
     2701 gtgtcgaagg ctatgagata tccgtggccg ggaatatcac tcccgcgtgg caggtgattg
     2761 gcggctatac ccagcaaaaa gcaaccatca aaaacggcaa agatgttgcc caggatggtt
     2821 cctcatcgct gcc
//
LOCUS       DAEVZA010000279         2828 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEVZA010000279 DAEVZA010000000
VERSION     DAEVZA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.280"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..2360)
                     /locus_tag="LAF92_005121"
     CDS             complement(<1..2360)
                     /locus_tag="LAF92_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9793992.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATRATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKLSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTNAGEAAVQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSSDITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSA"
     gene            complement(2425..>2828)
                     /locus_tag="LAF92_005122"
     CDS             complement(2425..>2828)
                     /locus_tag="LAF92_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBI9793993.1"
                     /translation="TSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAGPSVRVNEW
                     FSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSVDGRHSNTSLAWGAGVQFNP
                     TESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          532 a          823 c          744 g          729 t
ORIGIN      
        1 gcagatttgt caaaagtctg cccctgcatc agggcatagc cagacggaac ggtatctgat
       61 ggccacggga ttggtgcgcc aggcggataa aactgctctg atggcgtata gagtgaataa
      121 actgtaccgt ccgttaaccc ttccggctta ttagcagaat atgctggtga cgtatgaatc
      181 gtcacgctgg cattactggt ataatcccat tgaatattta caccagtcgc ataatttccg
      241 attgcaacgt aaatatcgta agtatcacca gatgtattga cccaggcaaa atttgtaaac
      301 cctgtcgatg tgcgctgcca taaagcacca gtaatcccct tcggattacc attacctgca
      361 cgcaaaacaa gttcagatat acctgcctgt tgaggtgacc ccacgttaaa tcccgcgcca
      421 ccaatcaacg taattgaaac aacagaactc gcctgtggca tggttaccgt tgctaatttg
      481 aaccaaccag caccaccgct gaatgacatt gttgttgagt taagcgtacc aatatctttc
      541 ggcgtcagtg ttatatccga tgaaagcgcc ttaccattca ccttacgggc agaaggtacc
      601 cgaccattcg cattatcatt agctgctttc actgctttcg gtgtcgcggc aagcgtttca
      661 gatgcgctgt tggttgcact actgagctgg acaattcctt tttgtgctgt cgtagcgtcc
      721 tgagcagtat atttcccgtt agcaaggtca taggctgcct ttaccgactt tggcgttgcc
      781 gccagcattt cagatgtgct gttggtcgcg ttgctaagct gaactatccc tttctgtgct
      841 gtcgttgcat cctgtgcggt gtatttcccg ttagccagct catacgcggc cttaacggct
      901 tttggcgttg ccgccagtga ctcggaagtg ctgttagtcg cactgctgag ttgtactatc
      961 ccctttttcg tcgtgctcgc atcctcaagc gccacggcgg atgcaatatc ctctgcccgt
     1021 tttgccgctg tctcggcgcg cgttgccgcg gattccgccg tacttttgct ctgagctgcc
     1081 gccgtcgcac tgccagctgc ctctgtcgcc ttcgtggatg ccgtcgtggc gctgcccttt
     1141 gctgctgacg cctgtctggt cgcctcatct tttgaagcag acgcagatga tgccgatgac
     1201 gccgccgaac tggcggacga tgcggcagcc gtttttgagg attctgcgct ggtttccgac
     1261 gctttcgcgt tcgtctcgga tgtcttcgct gcggaagcag acctcgctgc tgcgctggcc
     1321 tgtacagcgg cttcgccagc attcgttgtg gctgttgaag cagacgatgc agcactttct
     1381 gccgattttc cggcggcggt ggcactggct gaggcctgcc cggcacttgt tgacgcggca
     1441 ctggcagata atgcagccgc tgtttttgag cctgctgctg cggaggcact ctgttctgct
     1501 gccgtttcag aggacttagc gtttgtctca gacgttttgg ccgccttcgc ggaattgcct
     1561 gctgccgttg ccgaggaggc tgcactactg gcgctcgagg ctgcgctcgt ttctgataat
     1621 tttgccgcct cttttgaagc cgacgcatcc cgggctgagg tggcagcttc tgacgctttc
     1681 gtggtcgcgg tggatgcaga agtggctgct gattgttgtg acgctgccgc attcgtttct
     1741 gacgttttcg ccgcaccggc actggtggcc gccgcgcttt ttgaggactc agcagcggca
     1801 gcactttttg atgcttcagt ggcctttgtt gatgccgttc ctgcgctgga agacgctgac
     1861 tgagccgacg acgcggcctg tccggctgac gtgctggctg cgcgtgctga gcctgcagca
     1921 tcagtcgcac gggttgccgc ctcacgggca gatgtgctgg catcgctggc tgacttcttt
     1981 gcggctgccg tgttctgtgc caccgcggac gcgttacgcg ccacctcttc caccatcagt
     2041 tcaaaacgac gcagtgcctc cggacgggca tcatcctccg tcatggcacc gagaaaatca
     2101 ttcagcgtac cgggttgaga atcttcatac acggtgatgg tcccggcatg tgacggcggg
     2161 aatccctcca ccaacagaat aacgctgtac tgaccgtact caacgtccat gctgtaacgc
     2221 ccggcttcat ccggattttc tgaggccagc gtgttcacca ccaccgtggt actgttacgt
     2281 tttgctttca gctggattgt gcagttctgt accggttttc ctgtgccgtc tttcagtaca
     2341 cctgaaatct ttactgccat attcacccca caaaaaagcc cgcctgaacc ggcgggctgt
     2401 cataacgctg tgttacctgg ctaatcagaa tttataacca acacccacga tgaacccgtc
     2461 agtgcgccag tcaccactgc cggaaccttc ataagcgaca tcaatggcca cggattcggt
     2521 cgggttaaac tgcacgccag ccccccacgc cagagacgtg ttgctgtggc gaccgtcaac
     2581 acttccggtc agcacatcgt gcgttttccc cttgttgtca gttacgcgaa ggtaatcccc
     2641 ggaaaaagtc gaaacacggc tgtaagccat acccgccatc gcatacgcgc tgaaccattc
     2701 attcacgcgc acagacggcc ccgccatcac gctgaaccag cggttacgca cggaatcttc
     2761 atgccagcgg gtatcgctgt aacgggtcag ctggcgattc ttgtctcctg catagctgaa
     2821 tgacgtca
//
LOCUS       DAEVZA010000280         2763 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEVZA010000280 DAEVZA010000000
VERSION     DAEVZA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.281"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..231
                     /locus_tag="LAF92_005123"
     CDS             <1..231
                     /locus_tag="LAF92_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI9793994.1"
                     /translation="WHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTN
                     DKVFKAGLRRKMRKAAMGRNYLASVLTGSGLS"
     gene            261..713
                     /locus_tag="LAF92_005124"
     CDS             261..713
                     /locus_tag="LAF92_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9793995.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWRDPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKDAPQYPRGVRNVQNGTTRNVVKDQQLLKQLRKVDSGK"
     gene            931..1173
                     /locus_tag="LAF92_005125"
     CDS             931..1173
                     /locus_tag="LAF92_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HBI9793996.1"
                     /translation="MDEAEGFIFKPSTSKTLNAASHSKNLIDIIDSGCELEDISSIIP
                     ENLSGNIDKLINQTLDFIRGAPDFGGAVDKEIILVK"
     gene            1254..>2763
                     /locus_tag="LAF92_005126"
     CDS             1254..>2763
                     /locus_tag="LAF92_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI9793997.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPKKKD
                     SPNYGNPVNPVLGAKVLPGETDIALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSA
                     LWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDG
                     FGRTLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGP
                     RSASSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRF
                     EYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDE"
BASE COUNT          678 a          580 c          903 g          602 t
ORIGIN      
        1 tggcacgtgg agaataagct gcactggcgt ctggacgtgg taatgaatga agacgactgc
       61 aaaataagaa gaggaaatgc agcagaatta ttttcaggga tacggcacat cgctattaat
      121 attttgacga atgataaggt attcaaggca gggttaagac gtaagatgcg aaaagcagcc
      181 atgggcagaa actacctggc gtcagtcctt acggggagcg ggctttcgta atcttgccct
      241 gggtaggagg ccctccaggg atgttagtcg gtgcggcagt tggagcggtg gttattggtg
      301 gtggtatagt aatatacaac aatcgcgagg ctgttgggga tgcgattgag aaaagtatgg
      361 agatgcgggg tgaagccgaa ttattaaatg cacaaagtca aatggaattc tggcgagatc
      421 cctatggtgc tttttccagc gtgtttggaa atgatgattc atcaaaagag aatccaaatg
      481 tcgggaagga tttgacggat gaagagaaga actgtttggg cagtggcagt acaggaggtt
      541 cgggagggtg ggagccagat gaggatggcg aaataagaaa tacctacagg tctattaagg
      601 atgctcctca gtatcccaga ggggttagga atgtccaaaa tggcactaca aggaatgttg
      661 taaaggatca gcagttattg aaacaattac gtaaagtaga ttcaggaaaa tagagtaaag
      721 tttataaaga tggatttgat gcgtcaggtc gaaaagtgtc cattcactat tttgaaagtc
      781 agagtggtcg agtatttaat gtaaaagtaa aaccttattg gagtaattta taatgagaat
      841 tgattatagt attagcaatg aacacgcaga gaaatttatc agtcttttag tgttgggtgt
      901 tttatattct attgaaaaaa agcaatatct atagatgagg cagaaggttt tatatttaag
      961 ccatctacgt caaaaacatt gaatgctgca tcgcactcta aaaacttaat tgacattata
     1021 gattctggtt gtgagcttga agatatatct agcatcattc ctgaaaattt atcaggtaat
     1081 attgataaat taattaacca gacgctggat tttattcgag gtgctcctga ttttggcggt
     1141 gcagtcgata aggaaattat tttagtcaaa taaaaatata tcgttaatat atgttggtga
     1201 tgttttgttg agggcttttt tattgttagt ggtataaata taagggagtg agaatgagcg
     1261 gaaaaccggc ggcgcggcag ggcgacatga cccgcaaggg actggatatt gtgcagggtt
     1321 cagcaggggt gctgataggc gcgccgacgg gggttgcctg ctcggtgtgt cctaaaaaaa
     1381 aggacagtcc aaattatggc aacccggtga acccggtgct gggtgcaaag gtgctgccgg
     1441 gcgagacgga cattgcgctg cccggcccgc tgccgtttat tctttcccgc gcctacagca
     1501 gctaccggac cagaacaccc gcgccggtgg gggtgtttgg tcccggctgg aaagcgccct
     1561 tcgatatccg cttacagata cgcgatgaag gcctgatact caacgacagc ggcggcagga
     1621 gcattcactt tgagccgctg tttcccggcg agataagcta cagccgcagc gagtcactct
     1681 ggctggcgcg gggcggagta gcggcgcagc acagttcgca gccgctaagc gcgctctggc
     1741 aggtgctgcc ggaagatgtt cgcctgagtc cgcatgtgta cctggcgaca aacagccttc
     1801 aggggccgtg gtggatactc agctggccgg agcgggtgcc gggggcggac gaggtgctgc
     1861 cgccggagcc gcccgcatac cgggtgctga cgggcgtggt ggacggcttc gggcggacgc
     1921 tggcctttca ccgggcggcg gagggtgatg tggcgggcgc ggtgacgggg gtgacggacg
     1981 gcgcgggccg ccgttttcac ctggtgctga ccacacaggc acagcgggcg gaagtattcc
     2041 gtaaacagcg cgccacgtct ttatcttccc ctgccggtcc ccgttctgct tcctcttctc
     2101 tggttttccc cgacacgctg cccgccggta caggatacgg cacggataac ggtatccggc
     2161 tggaggcggt atggctgaca cacgacccgg catacccgga tgaacagccg actgcgccgc
     2221 tggcgcgcta cacgtacacg gccggtggtg aactgcgggc ggtgtatgac cgcagcggaa
     2281 cgcaggtgcg cgggtttact tatgatgcgg agcacgccgg gcggatggtg gcgcaccatt
     2341 atgcggggag gccggagagc tgctaccggt atgatgatac cggtcgggtg acggagcagg
     2401 tcaacccgga ggggctggac taccgctttg agtacggaga gagccgggta atcatcacgg
     2461 acagcctgaa ccggcgggag gtgctgtaca cggaaggcga gggtggcctg aagcgggtgg
     2521 tgaagaagga acatgcggac gggagcatta cccgcagcga gtatgatgag gcggggaggc
     2581 tgaaggcaca gacggatgcg gcggggcggc ggacggagta cagcctgcat atggcgtcgg
     2641 gtgcggtgac ggcggtgacg gggccggacg gcaggacggt acggtatggc tataacagcc
     2701 agcggcaggt gacgtcggtg acgtacccgg acgggcttcg cagcagccgg gagtatgatg
     2761 aga
//
LOCUS       DAEVZA010000281         2758 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEVZA010000281 DAEVZA010000000
VERSION     DAEVZA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.282"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>2758)
                     /locus_tag="LAF92_005127"
     CDS             complement(<1..>2758)
                     /locus_tag="LAF92_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBI9793998.1"
                     /translation="TSETNAAASQKSAATSASAATTKASEAATSARDAAASKEAAKSS
                     ETNASSSASSAASSATAAANSAKAAKTSETNAKASETAAGQSASAAAGSKTAAASSAS
                     AASTSAGQASASASAAGKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNA
                     KASETSAESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAG
                     SAAAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGVVQLSSATNSTSET
                     LAATPKAVKAAYDNAEKRLQKDQNGADIPDKDRFLSNINVYSKGEVDQKKGMRYVVVN
                     APAGVQEGKYYPLVIKRNDSHRASRVVISTPSRSANHRMNNCEFNGFVCAGGWTDRGS
                     YACGMFWAYSTSERAIHSILMSNKGDTVDSVFYIEGGAFPVEVFLEEGLSVTAPASDY
                     IVAETTYKFGATDPYSESVAVNLILDFKQGNGFYSSYPVLSKSDISGNKIYANDEVIV
                     RSQNALRMIAGDYGVIWRNDGANTYLLMTDKGDQYGGWNGLRPFAVNNATGEVTINTP
                     LNSPKGIKGNSDTATKLQTAIKISGVSFDGSTDITLTAAHVAAFARRATDTYADADGG
                     VPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEE
                     DWAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQAFDKSAYPKLAAAYPSGVI
                     PDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGAKTTSSFDYGTKSTNNT
                     GAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGW
                     LANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTV
                     KNIAF"
BASE COUNT          591 a          736 c          671 g          760 t
ORIGIN      
        1 aaatgcgatg tttttgacgg tgttttccgc gttaccagca gcgttaacgg tgatggtgtg
       61 tccatgtgaa ccaatcgcaa cggagtgcgt atgagcacca ataccgacag tatgcgcgtg
      121 tgcacctgca gatgctgctg tgccggacag tgagtggcta tgattaccat ctgtactggt
      181 attagctaac caccccgtag acatacctac tgagccttgt acaccccagg tattttgacc
      241 tgagcttgta taaccatatt gataagtatc tttaaaaaca ctggggttaa atcgacggcc
      301 atctctatgg ctgtgattac cagctgcatt cgtgctgcca cttaaactat gggtatgcgc
      361 accagtgtta ttcgtggatt tagtgccgta atcaaacgac gatgtggttt tcgcccccaa
      421 atccgtactg gatgcgctgg cgctgtgggt atgcgattta atgccgtcct gttcctgaga
      481 caatacggcc cgaccactgg caggtttgcc cttaatcgtc cagccacgca tatcagggat
      541 cacgcctgac ggataagccg ctgcaagttt cgggtatgca gatttgtcaa aagcctgccc
      601 ctgcatcagg gcataaccag acggaacggt atctgatggc cacgggattg gtgcgccgac
      661 tgggtagctt tctggtggaa gatttttcga ggtataaact tctgcccagt cttcctcaaa
      721 accatagcca tctcttgagg aacggtagaa cagacctcca tttctgtaat gtgccttcat
      781 ctgcaaggtc cggcaacttc cgactccggt atagaagtta accagaatat agctgtcgcc
      841 agagcgggtg acattgtaag cgcctgattc ggcattccat ggaacgcccc catccgcatc
      901 ggcatacgta tccgttgctc ttctggcaaa agcagcaaca tgcgcagcgg ttaaagtgat
      961 atcagtagaa ccatcaaacg aaacaccaga tatttttatt gctgtctgaa gtttcgtagc
     1021 tgtatcagaa tttcccttaa tccccttagg agaattaagt ggtgtattga tcgtcacttc
     1081 acctgttgcg ttatttactg caaatggtcg aagaccgttc cagccaccgt actggtcgcc
     1141 tttatcagtc atgagcaagt aagtatttgc tccatcattt cgccatatga caccgtaatc
     1201 acctgcaatc atccttaacg cgttctgact acggacaata acttcatcat tagcgtaaat
     1261 cttattcccg ctaatgtctg acttactcaa aacaggatac gaactataaa atccattacc
     1321 ttgtttaaaa tctaaaatta aatttaccgc aactgattca gaataaggat cagttgcgcc
     1381 aaatttataa gtcgtttcag caacaatata atcagaagca ggtgcagtaa cggagagacc
     1441 ttcttctaaa aagacctcta cgggaaatgc cccaccttct atatagaata cgctgtctac
     1501 ggtatcgcct ttattactca tcagaatgga atgaatagcg cgttccgatg ttgaataagc
     1561 ccagaacata ccacaggcat agctaccccg gtccgtccag ccgcctgcgc aaacaaaacc
     1621 attaaactca caattattca ttctgtgatt agctgagcga cttggcgttg aaatgacaac
     1681 gcgggatgcc cgatggctgt cattcctttt tatcactaac ggataatatt taccttcctg
     1741 gacacccgcc ggggcattta ccacaacgta tcgcatcccc tttttctgat ccacttcacc
     1801 tttgctgtaa acattaatgt tactcaggaa gcggtccttg tcgggaatat cagcaccgtt
     1861 ctggtctttc tgcagacgtt tctctgcatt gtcataggct gcttttactg cctttggcgt
     1921 tgccgccagc gtttcagacg tgctgttggt cgcactgctg agctgtacta cccccttttt
     1981 cgtcgtgctc gcatcctcaa gcgccacggc ggatgcaata tcctctgcac gttttgccgc
     2041 tgtctcagcg cgcgttgctg cggattctgc cgtacttttg ctctgtgctg ccgctgccgc
     2101 actgccagca gcctctgtcg ccttcgtgga tgctgtcgtg gcgctgccct tcgctgcgga
     2161 tgcttgtctg gtcgcctcat cttttgaagc agacgccgat gatgccgatg acgccgccga
     2221 actggcggac gatgcggcag ccgtttttga ggattctgcg ctggtttccg acgctttcgc
     2281 gttcgtctcg gatgtcttcg ctgcggaagc agacctcgct gctgcactgg cctgttcagt
     2341 ggcttcgcca gccttcgttg tggctgttga agcggacgat gcggcgcttt ctgccgattt
     2401 tccggcggcg ctagcactgg ctgaggcctg cccggcactt gttgacgcgg cactggcaga
     2461 tgatgcagcc gctgtttttg agcctgccgc agctgaggca ctctgtcccg ctgccgtttc
     2521 agaagccttg gcgttcgtct cggacgtttt tgccgccttc gcagaatttg ctgccgccgt
     2581 tgccgaggaa gcggcactac tggcgcttga tgatgcgttc gtttctgatg atttcgctgc
     2641 ctcttttgag gccgccgcat cccgggctga ggtggcagct tctgacgcct tcgtggtcgc
     2701 ggcggatgca gaagtggctg cagatttttg tgacgctgcc gcattcgttt ctgacgtt
//
LOCUS       DAEVZA010000282         2757 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEVZA010000282 DAEVZA010000000
VERSION     DAEVZA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.283"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..345
                     /locus_tag="LAF92_005128"
     CDS             <1..345
                     /locus_tag="LAF92_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBI9793999.1"
                     /translation="VLTRGDVRHIAQDWSLTDDELETVMQRLDDAFEHGADVSVVHDV
                     VRELMEEKRASRHVTVPAVMLEKVMALAGSEMKRLYAVGSENGGDGDAFVREEREAMD
                     VVLQALDGETMS"
     gene            342..533
                     /locus_tag="LAF92_005129"
     CDS             342..533
                     /locus_tag="LAF92_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794000.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            686..952
                     /locus_tag="LAF92_005130"
     CDS             686..952
                     /locus_tag="LAF92_005130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794001.1"
                     /translation="MIFRDTVTVYGCLLLRPLRLPAAWRHPVVRPDSDRQLLAHPPVS
                     AEPAGAVSGCRYGPMRENPGLQRPLRSRRPCRAVTCAPCTPDGV"
     gene            1304..1831
                     /locus_tag="LAF92_005131"
     CDS             1304..1831
                     /locus_tag="LAF92_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI9794002.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQSQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            1889..2122
                     /locus_tag="LAF92_005132"
     CDS             1889..2122
                     /locus_tag="LAF92_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBI9794003.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            2181..>2757
                     /locus_tag="LAF92_005133"
     CDS             2181..>2757
                     /locus_tag="LAF92_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBI9794004.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETALSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIQADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRI"
BASE COUNT          599 a          755 c          874 g          529 t
ORIGIN      
        1 gtgctgaccc gtggggatgt gcgtcacata gcccaggact ggagtctgac agacgatgag
       61 ctggaaaccg ttatgcagcg gctggacgat gcctttgagc atggtgcgga tgtcagcgtt
      121 gttcacgacg ttgttcgtga actgatggaa gaaaagcgcg ccagccgtca tgtgacagtc
      181 ccggcggtga tgctggaaaa agtgatggcg ctggcaggca gtgaaatgaa gcgcctgtat
      241 gccgtcggga gtgagaacgg gggcgacggt gacgcattcg tcagggagga acgcgaagca
      301 atggacgttg tgttacaggc gctggacggg gagacgatgt catgaatatc agcacagaaa
      361 cccgcgaaat tctgcgtaat tacagggccg tgattaatgc gcggcgtcgt gaaatggggc
      421 agaaaccgct caccaccgcg cagattgttg atgaaatctg cgattttgtg gcgaatcagc
      481 aggcggtttt cctgggcggt cactacatct tacagggcag cagaaacagg tgatacaggg
      541 gcaggcggtg gaggccgcct gtcagcccgc gcgaaaaccc ctggcaggct gacgcctgcc
      601 ggaaacgcgg ggtgcagcgc ctgcggcgct gccggcccgg ctttccggtc agaaggtagt
      661 gtatggctgt atgccaggaa cagatgtgat attccgtgac actgtcaccg tttatggttg
      721 tcttttattg cgcccacttc ggctcccggc tgcctggcgg catccggtcg tcaggcccga
      781 ctccgacagg cagctgctgg cgcacccgcc agtctccgcc gaacccgctg gcgcggtttc
      841 gggctgccgc tacggtccga tgcgtgaaaa cccgggcttg cagcgaccgt tacggtcgcg
      901 ccgcccctgt cgcgccgtga cctgcgctcc ctgcacacct gacggtgtgt gaggaaactg
      961 cgcctgatgg cgcagcagtc ctccctcctg ccgccacccc tgcggcctgt ttcccgctgt
     1021 ggactttccg gggggcagac gtaaccccct cccgttccat ctgtccaggg tgagatgcac
     1081 cggccacaaa atttttagcc atgaacccgg caaaaaattt tgcgtccgcc cccggacagc
     1141 tgtcccgtga ggcggtaaac gggcccccat cggaaaggtc cactgcgggg agaaaccccg
     1201 aagaagtgga cggcaggagg agctttcgct cctctgaagg ggcacatgaa cgtcccgtcg
     1261 ccggtatccg gtttatctca catctttcag tcaggaggtt cttatgagtg cacgtggtat
     1321 caacaaggtc atcctcgtcg ggcgtctggg caatgatccg gaagtccgtt acatccccaa
     1381 cgggggcgca gtggcaaacc tgcaggtggc cacatcagaa agctggcgtg acaaacagac
     1441 gggggagatg cgggagcaga cggaatggca ccgcgtggtg ctgttcggca agctcgcgga
     1501 agtggcaggt gaatatctgc gcaagggtgc gcaggtctac atcgaaggtc agcttcgcac
     1561 ccgtagctgg gaagataacg gtatcacccg ttacgtcact gaaattcttg ttaagaccac
     1621 gggcaccgtg cagatgctgg gacgtgcacc acagcagaac gctcaggcgc aaccgaagcc
     1681 tcagcagaat gggcagtcac agagtgctga cgcgacgaaa aaaggtggcg cgaaaacgaa
     1741 aggccgtgga cgtaaggccg cgcagccaga gcctcagccg caaccgccgg agggggagga
     1801 ttacgggttt tcagacgaca tcccgttctg atcgggctga ctgtgacaac cgccccgccc
     1861 tgtgcggggc atcaccggag atatgaggat gagcgaatat ttcagaatac ttcagggact
     1921 gccggacggc tcctttaccc gcgaacaggc ggaagccgtt gccgcacagt accggaacgt
     1981 ctttatcgag gatgatcagg gaacgcattt tcgcctggtt gtccgtcagg atggcacgtt
     2041 gatctggcgc tcctggaatt ttgaggacgg tgccgggtac tggatgaacc agtacatcag
     2101 ggatttcggg attcttaagt aagagaggtg ccggacgcgc gaacgtccgg caggacataa
     2161 gcaattataa ggggatgatt atgcctgtaa cgaagtgtga accagaaact acccgcaaag
     2221 caagccgtaa atctgtaaaa acgcaggaaa ctgcactgtc tgccctgctg gcgcagacgg
     2281 aggaagtgag cgtgccgctg gattcactga ttaaatcacc gctgaatgtg cgcacggtgc
     2341 cgtattctgc ggagtccgtc agtgaactgg ctgattccat taagggagtc ggcctgctgc
     2401 agaatctggt tgttcatgcc ctgccaggtg accgttacgg tgtcgccgca ggtggtcgcc
     2461 gactggcagc actcaacatg ctggcagaac gtgacatcat tcaggctgac tggcctgtcc
     2521 gtgtgaaggt cattccgcag gagctggcga ctgccgcatc gatgaccgag aacggtcatc
     2581 gtcgggatat gcaccctgcc gaacagattg ccggattccg cgcaatggcg caggaaggca
     2641 aaacacctgc acaaatcggt gatttgctgg gctattcacc ccgccacgtt cagcgaatgc
     2701 tgaaactggc tgaccttgcg cctgtcatcc tcgatgcgct ggcagaagac cgcatca
//
LOCUS       DAEVZA010000283         2691 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEVZA010000283 DAEVZA010000000
VERSION     DAEVZA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.284"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..2114
                     /locus_tag="LAF92_005134"
     CDS             <1..2114
                     /locus_tag="LAF92_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI9794005.1"
                     /translation="THRTVNGDPAEQWQYDEHGWLTTLSHTCEGHRVSVHYGYDDKGR
                     LTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKL
                     GGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYTLTGQLQSRHLNLPQLDCDYTW
                     NDNGQLVRISGPQECREYRYSGTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELHP
                     DSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHRL
                     VFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEETWYGWDGDR
                     LTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEVLQEDTGVTLPAELA
                     VMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLHLYHCDHRGLP
                     LALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRLPGQQYDEESGLYYNRNRYYDP
                     LQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKFHVNGDPSDFNQAVEYLKQDSQ
                     MKETIDFLSSSEETINIEYIEGTNVRFNSNNMAIYWNSRASLFCSTELNSKSQSPALG
                     LGHEFAHAQYYLLDKENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHS
                     GLPFYRVDGPLQTMKITGTPE"
     gene            2126..2575
                     /locus_tag="LAF92_005135"
     CDS             2126..2575
                     /locus_tag="LAF92_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794006.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          736 a          565 c          833 g          557 t
ORIGIN      
        1 tcacgcaccg gacggtgaac ggcgacccgg cagagcagtg gcagtatgat gagcacgggt
       61 ggttaaccac cctcagccat acctgtgaag gccaccgcgt gtcggtccac tacggctatg
      121 acgataaagg ccgcctgacg ggtgaacggc agacggtgga gaacccggag acaggggaga
      181 tgctgtggga gcatgagacg gggcatgcgt acagcgaaca ggggctggcg acccgtcagg
      241 agccggacgg tctgccgccg gtggagtggc tgacgtatgg cagcggttat cttgcgggga
      301 tgaagctggg cggaacgccg ctggtcgagt acacgcggga ccggctgcac cgcgagacgg
      361 cccgtagctt cggcggggcg ggcagtacag cggggtacga acaggccacg gcgtacaccc
      421 tcacagggca gctacagagc aggcacctga accttccgca gcttgactgt gactatacgt
      481 ggaacgataa cggacaactg gtgcgcatca gcggcccgca ggagtgccgg gagtaccgtt
      541 acagcggcac gggaaggctg acgggcgtcc acactacggc agcgaacctg gatatcgata
      601 tcccgtatgc gacggacccg gcaggaaacc ggctgccgga cccggaactg cacccggaca
      661 gcaccctcac ggcgtggccg gataaccgca tcgcggaaga tgcgcactat gtctaccgct
      721 acgatgaata cggcaggctg gcggagaaga cggaccgcat cccggaaggg gtgatacgga
      781 tgcacgacga gcggacccac cactaccact acgacagcca gcaccgtctg gtgttctaca
      841 cgcggataca gcacggtgag ccgcaggtgg agagccgcta cctctatgac ccgctgggcc
      901 gccggacggg aaaacgggtg tggcggcggg agcgtgacct gacggggtgg atgtcgctgt
      961 cgcgtaaacc ggaggagacc tggtacgggt gggacggtga caggctgacc actgtacaga
     1021 cccaacagac aagaatccag acggtatacc agccgggaag cttcacgccg ctcctgagaa
     1081 tcgaaacaga gaatggtgaa caggcgaagg cgcggcaccg tagcctggcg gaggtgttgc
     1141 aggaggacac gggtgtgacg ctaccggcgg agctggcggt gatgctgggg aggctggagc
     1201 gggaactgcg gcagggcagc gtgagtgaag aaagccagca gtggcttgcg cagtgcgggc
     1261 tgacggcgga acagatggcc gcgcaactgg aggcggaata catcccggag aggaaacttc
     1321 atctttacca ctgtgaccac cgtgggctgc cgctggcgct tatcagcccg gaaggggaaa
     1381 cggcgtggca gggggagtat gacgagtggg gaaacctgct gggcgaggaa agcgcgcagc
     1441 accttcagca gtcactccgt ctgccgggac agcagtatga tgaggagtcg gggctgtact
     1501 acaaccgcaa ccggtactat gatccgttgc aggggagata tatcacccag gacccgatag
     1561 ggctgagagg tgaatggaat ctgtataaat atccattaaa ccctgttcgg tttatagatt
     1621 ctttgggttt aaaattccat gttaacggtg atccaagtga ttttaatcag gctgttgagt
     1681 atttaaaaca agattcacaa atgaaagaaa ctatagattt tttatcaagc tctgaagaaa
     1741 ctataaatat agagtatatt gaaggaacca atgttagatt taattcgaat aatatggcga
     1801 tatattggaa tagtagagca tctttatttt gttcaactga attaaactcg aaatcacaat
     1861 cgcctgcatt aggtttggga catgagtttg ctcatgcgca gtattacctt cttgataagg
     1921 aaaatttcat ggctctttta agccgaacag ataagaaata tgagaataaa gaagaggcaa
     1981 gggttattac aataattgaa tcgcgtgcgg cgaaaacttt aggtgagtgt accagaggag
     2041 ctcatagtgg actgcctttt tatcgggtgg atggaccgtt gcagacgatg aaaataacgg
     2101 gtacgccaga atagtgaggt tttttatgat gaaatatttg atggttttat tgtctctatt
     2161 ttctggttcg gtactgggga tggggcgagt gaatgaattg tgtggaatag atagcgtgaa
     2221 aacgatagag ataattaatc ttccatcgta tgttaccacg ttagttccgc ttagcaagga
     2281 aggtcttaat gaaatataca gatataaagt ggtggtgaat gaaattagcg atctttatgc
     2341 tggtaagata attgatttat tgcaaatgaa gtatttcaga aaagaaaaat ataacaatat
     2401 aagatggggg gttagtatca tttcgaaagg taataataaa tgtgaaattt attttgatgc
     2461 ttttggtgaa tgtggaagtg tgaatggcat taacgtgtgt tttgaaaaaa atgaaatgat
     2521 aggttggatt aaaaaagaaa tccctttgct gtcgcaaaaa ataggtgggc tttaaatggt
     2581 agggcaatat tcaaagacag tataggtaac tatttgtatg gccatgtaag gtaaaagaaa
     2641 aggaataaac catgagcgga aaaccggcgg cgcggcaggg cgacatgacc c
//
LOCUS       DAEVZA010000284         2670 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEVZA010000284 DAEVZA010000000
VERSION     DAEVZA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.285"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1590
                     /locus_tag="LAF92_005136"
     CDS             <1..1590
                     /locus_tag="LAF92_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="cleaves origin of transfer and unwinds; oriT
                     relaxase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBI9794007.1"
                     /translation="LIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRD
                     GELRRLSTWETHRDALALVDNVYHRIAGISRDDGLITLQDAEGNTRLISPREAVAEGV
                     TLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPAQ
                     EQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQV
                     YTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDVAAGRAVLRQ
                     AGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTSDDGNGLRGFSGEG
                     RVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAI
                     TGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKP
                     DLPDGKTEQAVREIAGQVRERAAITERETALPESVLREPQREREAVREVARENLLQER
                     LQQMERDMVRDLQKEKTLGGD"
     gene            1610..2356
                     /gene="traX"
                     /locus_tag="LAF92_005137"
     CDS             1610..2356
                     /gene="traX"
                     /locus_tag="LAF92_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBI9794008.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            2411..>2670
                     /gene="finO"
                     /locus_tag="LAF92_005138"
     CDS             2411..>2670
                     /gene="finO"
                     /locus_tag="LAF92_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBI9794009.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPG"
BASE COUNT          583 a          675 c          910 g          502 t
ORIGIN      
        1 ctgattgtca cgcacctgaa tgaggaccgg cgcgtgctga acagcatgat tcatgatgca
       61 cgggaaaagg ccggtgagct gggaaaagag caggtcatgg tgcctgtcct gaacacagcg
      121 aatatacgtg acggagaact gcgtcgtctt tccacctggg agacacatcg ggacgcactt
      181 gccctggtgg ataatgtgta tcaccggatt gccggtatca gcagggatga cgggctgata
      241 accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc ggtggctgaa
      301 ggtgtcacac tgtacacccc ggacaaaatc cgggtgggga ccggtgaccg gatgcgcttc
      361 acgaagagtg accgggagcg tggttatgtg gccaacagcg tctggacggt gacagcggta
      421 tccggtgaca gtgtcacgct gtcggacgga cagcagaccc gggtgattcg ccccgcccag
      481 gagcaggcag agcaacatat tgacctggcc tatgccatca ccgcccacgg tgcgcagggt
      541 gcgagtgaaa cctttgccat tgcgcttgaa ggcacggaag gtaaccggaa actgatggcc
      601 ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca ggtgtacacc
      661 gataaccgtc agggctggac ggatgccatt aacaatgccg tacagaaagg aactgcccac
      721 gatgtgctgg agccgaaacc ggaccgggag gtcatgaatg cagagcggct gtttagtacg
      781 gcgcgggagc tgcgggacgt ggcggcaggg cgtgccgttc ttcgtcaggc ggggcttgcc
      841 gggggagaca gtcctgcacg gtttattgca ccggggcgta aatatccaca gccgtatgtg
      901 gcactgccgg cgtttgaccg taacggcaaa tcagccggta tctggctgaa cccgctgacc
      961 tcggatgatg gaaacgggct gcggggattc agtggtgaag gccgtgtgaa aggcagcggg
     1021 gatgcgcagt tcgtggccct gcagggcagc cgtaacggag aaagcctgct ggctgataat
     1081 atgcaggatg gtgtccggat tgcccgtgat aatcctgaca gtggtgtggt ggtaagaatc
     1141 gccggtgaag gccgtccgtg gaatcccggt gccatcaccg gtggccgcgt gtggggggat
     1201 atcccggaca acagcgtcca gccgggagcc ggaaatggcg agccggtcac ggcagaggta
     1261 ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgccg tgcggatgaa
     1321 attgtccgta aaatggcaga gaacaaacct gacctgccgg atggcaaaac agagcaggct
     1381 gtcagggaga ttgccgggca ggtgcgtgag cgggctgcca taactgaacg ggaaactgcg
     1441 ctgccggaga gtgtgctgcg tgaaccacaa cgggagcggg aagcggtccg ggaggttgcc
     1501 cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt ccgtgacctg
     1561 cagaaagaga aaaccctggg cggagactga tacaggaagg caaacgctga tgacaacgga
     1621 taacacgaac acgacacgta acgattcact ggctgcccgg accgatacct ggttgcagtc
     1681 gttgctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat
     1741 ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt ttctggccgg
     1801 gcgtggagcc tttccgctgt ttgccctggt gtgggggctg aatctgtccc gtcatgcgca
     1861 tatccggcaa ccggccatta accggctgtg gggatggggg attattgccc agttcgcata
     1921 ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg cagtggcggc
     1981 acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccattcttct
     2041 gatggcgctg tgggggcctt tgtccggcac cagttacggc attgccgggc tgctgatgct
     2101 ggcagtcagc caccggctgt accgggcgga agacagagcg gaacgtctgg cgctggtcgc
     2161 ctgcctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag cggcggtggc
     2221 cggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa aatcattacc
     2281 ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg ctgtgctggg
     2341 cgttctggcg ctgtgacggg tgtggtatct ttgaccgcaa gaggatgatt cgtcagaggc
     2401 agaacacagc atgacagagc agaaacgacc ggtactgaca ctgaagcgga aaacagaagg
     2461 agagacgcct gtccgcagcc ggaaaaccat catcaatgtc accacgccac caaaatggaa
     2521 ggtgaaaaag cagaaactgg ccgagaaagc ggcccgggaa gcagagctgg cggcaaaaaa
     2581 agcgcaggcc agacaggcgc tgtccattta tctgaacctg ccctcactgg atgaggccgt
     2641 gaataccctg aagccctggt ggccgggatt
//
LOCUS       DAEVZA010000285         2554 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEVZA010000285 DAEVZA010000000
VERSION     DAEVZA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.286"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            123..>2554
                     /gene="traC"
                     /locus_tag="LAF92_005139"
     CDS             123..>2554
                     /gene="traC"
                     /locus_tag="LAF92_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBI9794010.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYRV
                     FFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLREKGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKKNKARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHR"
BASE COUNT          607 a          665 c          696 g          586 t
ORIGIN      
        1 catgagtgga tattcagaaa acagaaatct ttgtatatgc gccgggaaat ggcgcgttaa
       61 ttacaggtat tcccttcatg gctaccgcat ttctcgcttt atttttcaac taaggaattc
      121 atgtgaataa tccacttgag gccgtcactc aggcggttaa ctccctcgtc acagcactga
      181 aactgcctga cgaatccgca aaggccaatg aagttctggg cgaaatgagc ttcccgcagt
      241 tcagtcgtct gctgccgtac cgtgattaca accaggaatc cggtctgttc atgaatgaca
      301 ccacgatggg ctttatgctg gaagccattc ccatcaatgg ggcgaatgag tccattgtgg
      361 aggccctcga tcacatgctg cgcaccaaac tgccgcgcgg ggtgccgttc tgcatccatc
      421 tgatgtccag tcagctggtt ggtgacagga ttgaatacgg gctgcgtgag ttctcctggt
      481 cgggtgaaca ggctgaacgg tttaacgcca ttacccgtgc ctattatatg aacgcggcag
      541 cgacacagtt tccgctgccg gaggggatga atctgcccct gaccctgcgc cattaccggg
      601 tattcttctc gtactgttct ccctcgaaga aaaaaagccg ggctgatatt ctggaaatgg
      661 aaaacctggt gaaaatcatc cgggcgtcgt tacagggggc cagtatcacc acacaggcgg
      721 tggatgcaca ggcctttatc gatattgtcg gggagatgat taaccataac ccggactccc
      781 tgtatccgaa aagacgtcag ctggacccgt attctgatct gaattatcag tgtgtggagg
      841 acagttttga cctgaaagtc cgggcggatt acctgacgct gggcctgcgt gagaagggta
      901 ggaacagcac ggcccgcatc ctgaatttcc atctggcccg taacccggaa atcgccttcc
      961 tgtggaacat ggccgacaac tacagcaacc tgctgaaccc ggaactgtcc atctcctgtc
     1021 cgttcatcct gacgctgact ctggtggtgg aagaccaggt gaaaacccac agcgaagcca
     1081 acctgaagta catggacctg gagaaaaagt cgaagacctc ctatgccaaa tggtttccgt
     1141 ccgtggagaa agaggcgaag gagtgggggg aactgcgtca gcggctgggc tccggtcagt
     1201 cttctgtcgt gtcctacttc ctcaacatca cagcattctg caaggacaac aatgaaacgg
     1261 cgctggaagt ggagcaggac attctgaaca gctttcgtaa aaacggtttt gagctgattt
     1321 caccgcgctt taaccacatg cgcaatttcc tgacctgtct gccctttatg gccgggaaag
     1381 ggctgtttaa acagctgaaa gaggcaggtg tggttcagcg cgcagagagc tttaatgttg
     1441 ccaacctgat gccgttagtg gcggataacc ccctgacacc ggcaggtctg ctggcaccca
     1501 cctaccgtaa ccagctggcg tttatcgata ttttcttccg ggggatgaat aacaccaact
     1561 acaacatggc ggtctgtggc acctccgggg ccggtaaaac cgggctgata cagccactta
     1621 tccgcagcgt gctggactcc gggggctttg ccgtggtgtt cgacatgggg gatggctata
     1681 agtccctgtg tgagaacatg ggtggggtgt atctggacgg tgaaaccctg cgttttaacc
     1741 cgtttgcgaa catcaccgat attgaccagt cagcggagcg tgtccgtgac cagttgtcgg
     1801 tgatggccag ccccaacggt aacctggatg aagtacatga aggtctgctg ctgcaggcgg
     1861 tcagggcctc ctggctggct aaaaagaata aggcgcgcat cgatgatgtg gtggatttcc
     1921 tgaaaaacgc ccgtgataac gaccagtatg tcgagtcacc gaccatccgc agccgtctgg
     1981 acgaaatgat tgtgctgctt gaccagtaca ctgccaacgg cacttacggc cagtatttta
     2041 actctgatga gccgtccctg cgggatgacg caaaaatggt ggtgctggag ctgggcggac
     2101 tggaagaccg tccgtcactg ctggttgcgg tgatgttcag ccttatcatt tacatcgaga
     2161 acaggatgta ccgcacgccg cgtaatctca aaaaactgaa cgttattgat gaaggctggc
     2221 gtctgctgga cttcaaaaat cacaaggtcg gtgaatttat tgagaaaggt taccgtaccg
     2281 cccgccgtca taccggtgcc tatatcacca tcacacagaa catcgtcgac tttgactctg
     2341 acaaggcctc cagtgctgca cgcgcggcat ggggtaactc ctcctacaaa attatcctca
     2401 aacagagtgc gaaggagttc gcgaaataca accagctgta tccggaccag ttcctgccac
     2461 tgcagcgtga catgattggt aagtttggtg cggccaaaga ccagtggttc agttccttcc
     2521 tgctgcaggt ggaaaaccat tcctcctggc accg
//
LOCUS       DAEVZA010000286         2543 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEVZA010000286 DAEVZA010000000
VERSION     DAEVZA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.287"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1..710
                     /locus_tag="LAF92_005140"
                     /pseudo
     CDS             1..710
                     /locus_tag="LAF92_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            608..1255
                     /locus_tag="LAF92_005141"
     CDS             608..1255
                     /locus_tag="LAF92_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBI9794011.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1316..>2543
                     /locus_tag="LAF92_005142"
     CDS             1316..>2543
                     /locus_tag="LAF92_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBI9794012.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNIVGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GMQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQHCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNG"
BASE COUNT          529 a          647 c          848 g          519 t
ORIGIN      
        1 gcccggcgat gtgcgccagc ggagtcgtgc ggcttcgtgg tgagaacacc ggagggggaa
       61 agatattttc cctgcgtgaa tatctccggt gagccggagg agtatttccg gatgtcgccg
      121 gaggactggc tgcgggcaga gatgcagggt gagattgtgg cgctggtcca cagccacccc
      181 ggtggtctgc cctggctgag tgagaccgac cggcggctgc aggtgcagag tgatttgccg
      241 tggtggctgg tttgccgggg gcgattcaca agttccgctg tgtgccacat ctttccgggc
      301 ggcgctttga gcacggggtg acggactgtt acacgctgtt ccgggatgct taccatctgg
      361 cggggattga gatgccggat tttcatcgcg aggatgactg gtggcgtcac ggtcagaatc
      421 tctatctgga taatctggag gccacagggc tgtatcaggt gccgttgtca gcggcgcagc
      481 cgggcgatgt gctgctgtgc tgttttggtt catcggtgcc gaatcatgcc gccatttact
      541 gtggtgacag cgagctgctg caccatattc ctgaacaact gagcaaacga gagaggtaca
      601 ccgacaaatg gcagcgacgc acacactccc tctggcgtca ccgggcatgg cacgcatctg
      661 cctttacggg gatttacaac gatttggccg ccgcatcgac cttcgagtga aaacggggtc
      721 cgaagccatc cgggcgctgg ccatgcagat cccggcgttt cgtcagaaac tgagcgacgg
      781 ctggtaccag gtacgcattg ccgggcgtga tgccggtgaa accgaattgt ctgcccgtct
      841 taatgagccg ctggcaaatg gtgccgtgat ccacatcgtg ccgcgtctgg cgggagctaa
      901 aagtggcggt gtgtttcagg tggtgctggg ggcggctctg attgcggtgg catggtggaa
      961 ccctgtgggc tggctgggtg ccgcggctgt atcgggcatg tatgcggcag gggccagtat
     1021 gatcctgggc ggagtggcgc agatgctggc accgaaagcc aggacgccca cggcagcaag
     1081 tacagataac ggcaaacaga acacctattt ctcctcactg gataacatgg ttgcccaggg
     1141 caatgttctg cccgttctgt acggtgaaat gcgcgtgggg tcgcgggtgg tctctcagga
     1201 gatcagcacg gcagacgaag gggatggtgg tcaggttgtg gtgattggtc gctgatgcaa
     1261 aacattttat gtgaaaccgc ctgcgggcgg ttttgtcgtt tatggagcgt gatgaatggg
     1321 taaaggcagc agtaaggggc ataccccgcg cgaagcgaag gacaacctga aatccacgca
     1381 gttgctgagt gtgattgatg ccatcagtga agggccgatt gaaggtccgg tggatggatt
     1441 aaaaagcgtg ctgctgaaca gtacgccggt gctggacagt gaggggaata ccaacatcgt
     1501 cggtgtcacg gtggtgttcc gggccggtga gcaggagcag actccgccgg agggatttga
     1561 atcctccggc tccgagacgg tgctgggtac ggaagtgaaa tacgacacgc cgatcacccg
     1621 caccatcacg tcggcaaaca tcgaccgact gcgctttacc ttcggtgtgc aggcactggt
     1681 ggaaaccacc tcaaaggggg accggaatcc gtcggaagtt cgcctgctgg ttcagataca
     1741 acgtaacggt ggctgggtga cggaaaaaga catcaccatt aagggcaaaa ccacctcgca
     1801 gtatctggcc tcggtggtgg tggataacct gccgccgcgc ccgtttaata tccggatgcg
     1861 cagaatgacg ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcata
     1921 caccgaaatc atcgatgtga aacagtgcta cccgaacacg gcactggtcg gcatgcaggt
     1981 ggattcggag cagttcggca gccagcaggt gagccgtaat tatcatctgc gcgggcgcat
     2041 tctgcaggtg ccgtcgaact ataacccgca gacgcggcaa tacagcggta tctgggacgg
     2101 aacgtttaag ccagcataca gcaacaacat ggcctggtgt ctgtgggata tgctgaccca
     2161 tccgcgctac ggcatgggga aacgtcttgg tgcggcggat gtggacaaat gggcgctgta
     2221 tgtcatcggc cagcattgcg atcagtcggt gccggacggt tttggcggca cggagccgcg
     2281 catcacctgt aatgcgtacc tgaccacaca gcgcaaggcg tgggatgtgc tcagtgattt
     2341 ctgctctgcg atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga
     2401 ccgaccgtcg gataaggtgt ggacctataa ccgcagtaat gtggtgatgc cggatgatgg
     2461 tgcgccgttc cgctacagct tcagcgccct gaaggaccgc cataatgccg ttgaggtgaa
     2521 ctggattgac ccgaataacg gct
//
LOCUS       DAEVZA010000287         2521 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEVZA010000287 DAEVZA010000000
VERSION     DAEVZA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.288"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            127..1002
                     /locus_tag="LAF92_005143"
     CDS             127..1002
                     /locus_tag="LAF92_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-55"
                     /protein_id="HBI9794013.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(1049..1525)
                     /locus_tag="LAF92_005144"
     CDS             complement(1049..1525)
                     /locus_tag="LAF92_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HBI9794014.1"
                     /translation="MKQITMSDMQQQSAAAVQSPRLRAHRNFHPELSDSVQRLAIAME
                     PGTYVRPHRHPHTFELLLPLRGRFVVLNFDDRGTVTHRAILGETCTVLEMAAGTWHAV
                     LSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTELMAWYAQAQVGDSTFAV"
     gene            complement(1784..>2521)
                     /gene="bla"
                     /locus_tag="LAF92_005145"
     CDS             complement(1784..>2521)
                     /gene="bla"
                     /locus_tag="LAF92_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HBI9794015.1"
                     /translation="VGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAG
                     QEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGP
                     KELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLAS
                     RQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVI
                     YTTGSQATMDERNRQIAEIGASLIKHW"
BASE COUNT          602 a          639 c          662 g          618 t
ORIGIN      
        1 ttcattctgg cgacgtccgt atttgccttt cggaagcata aaatcggacg cgttgtggct
       61 cgcttcaggt aaaatattga ctattcatgt tgttgttatt tcgtctcttc cagaataagg
      121 aatcccatgg ttaaaaaatc actgcgccag ttcacgctga tggcgacggc aaccgtcacg
      181 ctgttgttag gaagtgtgcc gctgtatgcg caaacggcgg acgtacagca aaaacttgcc
      241 gaattagagc ggcagtcggg aggcagactg ggtgtggcat tgattaacac agcagataat
      301 tcgcaaatac tttatcgtgc tgatgagcgc tttgcgatgt gcagcaccag taaagtgatg
      361 gccgtggccg cggtgctgaa gaaaagtgaa agcgaaccga atctgttaaa tcagcgagtt
      421 gagatcaaaa aatctgacct tgttaactat aatccgattg cggaaaagca cgtcaatggg
      481 acgatgtcac tggctgagct tagcgcggcc gcgctacagt acagcgataa cgtggcgatg
      541 aataagctga ttgctcacgt tggcggcccg gctagcgtca ccgcgttcgc ccgacagctg
      601 ggagacgaaa cgttccgtct cgaccgtacc gagccgacgt taaacaccgc cattccgggc
      661 gatccgcgtg ataccacttc acctcgggca atggcgcaaa ctctgcggaa tctgacgctg
      721 ggtaaagcat tgggcgacag ccaacgggcg cagctggtga catggatgaa aggcaatacc
      781 accggtgcag cgagcattca ggctggactg cctgcttcct gggttgtggg ggataaaacc
      841 ggcagcggtg gctatggcac caccaacgat atcgcggtga tctggccaaa agatcgtgcg
      901 ccgctgattc tggtcactta cttcacccag cctcaaccta aggcagaaag ccgtcgcgat
      961 gtattagcgt cggcggctaa aatcgtcacc gacggtttgt aatagcggaa acggaatggg
     1021 gaaactcatt ccgtttttgt ttatcgcctt agacggcaaa agtgctgtcg cccacctgcg
     1081 cttgcgcata ccaggccata agctccgtgg ttcctggttc tccttccgct ggagcccagt
     1141 gcgcatagtc atcggcagcc acgggttgat agccaccgtg ttttacttca aaaattatgc
     1201 caccggtatc cagcgacagc acggcatgcc aggttcctgc ggccatctcc agcaccgtac
     1261 aggtttcccc caatatcgcc cgatgggtga cggtaccccg atcgtcaaaa ttcagcacca
     1321 cgaaacgacc ccttaatggc aacagtagct cgaaggtgtg agggtgtcgg tgcgggcgca
     1381 cgtaggtccc aggttccatg gcaatagcca gacgttggac cgaatcgctt aattctggat
     1441 ggaaattacg gtgcgcgcgg agccgaggcg attgcacagc cgctgcgctc tgttgctgca
     1501 tatcgctcat ggtaatttgt ttcatcagga tgaccttgtc gattaatgaa ttggggggga
     1561 ttaccgtatg acgtcagggt gacagaaccc gcagagcaag tgaagaactg ggtttgccaa
     1621 tttttcccta tcctgattta ctgaaatggt attgacggta gtgctgatat tcaggccact
     1681 tttttagttg ataaaagtct gtaattcttt ttaccgtctg ggaaaaattt acggtggtaa
     1741 tcacccccca attatttcag tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1801 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1861 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1921 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1981 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2041 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     2101 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2161 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2221 ctccgatcgt tgtcagaagt aagttggcag cagtgttatc actcatggtt atggcagcac
     2281 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2341 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2401 cacgggataa taccgcacca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2461 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2521 c
//
LOCUS       DAEVZA010000288         2493 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEVZA010000288 DAEVZA010000000
VERSION     DAEVZA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.289"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            321..737
                     /locus_tag="LAF92_005146"
     CDS             321..737
                     /locus_tag="LAF92_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosfomycin resistance glutathione transferase
                     FosA3"
                     /protein_id="HBI9794016.1"
                     /translation="MLQGLNHLTLAVSDLASSLAFYQQLPGMRLHASWDSGAYLSCGA
                     LWLCLSLDEQRRKTPPQESDYTHYAFSVAEEEFAGVVALLAQAGAEVWKDNRSEGASY
                     YFLDPDGHKLELHVGNLAQRLAACRERPYKGMVFFD"
     gene            complement(742..1260)
                     /locus_tag="LAF92_005147"
     CDS             complement(742..1260)
                     /locus_tag="LAF92_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004108661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794017.1"
                     /translation="MRLAPRPFFALCVLVFIAAAAFSAWRLLPADNAGVMSCSTKGIM
                     RFENMDKENVNGNIHFNFGAQGKGSMVVEGYTDSAAGWLYLQRYVKFTYSSKRISATE
                     RHYRINQWESSASSIDESPNVIFDYFMREMSDSHDGLFLNAQKLNEKAILLSSINSPL
                     WICTLKSGSKLN"
     gene            complement(1260..2048)
                     /locus_tag="LAF92_005148"
     CDS             complement(1260..2048)
                     /locus_tag="LAF92_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBI9794018.1"
                     /translation="MRYNINARFIYDATDGTLTLPQSDEPDSQLSLTASALFNYFLRH
                     TEIVSREDVLKKVWDDNGLTSSNSNLNQYLSMLRKTFRHYGVDNIIVTVSRGYLQLNP
                     EVIIEALDDAPPVSPQQTPAEPPPVTTTPDAPRSAHARGMCWYMAGACLLTIALLLVA
                     FSLVGISESQPIALTRLSHSQCELLASDEMRRSVSEESYGKNFDEVRQRLNIACKPGE
                     RFLFFYGDRLETNGLGRVFLAHCAMHEDNPFSYCDNYFYYSWKP"
     gene            complement(2068..>2493)
                     /locus_tag="LAF92_005149"
     CDS             complement(2068..>2493)
                     /locus_tag="LAF92_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004133049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI9794019.1"
                     /translation="KGGLIHHFPNKQALIFALFARLLAIMEEAITALMQQDGVSYGRF
                     TRAYLNYLADLTDTHESRQLMVLSLAMPDEPVLRKCWRDWMLEKLAQGDELDNSPTGT
                     LVRYAADGIWLSELTEGITMSADHRRALVDSLNKMTLPA"
BASE COUNT          537 a          614 c          765 g          577 t
ORIGIN      
        1 aattatttag gatatagctc tgtgatttag tctttttagg gcgtttattt atgtctttat
       61 gctgtaatgt cggtgctgat ttggtcttca ataaggcgat ttttagcccg ctttttccgg
      121 ggggatgaaa tgttattgga ttgctaaatg atttcaaaaa aggggtaaca tagagcgggg
      181 attagtgtgg cgaggcgcag gtttccgatg gcaggctaaa cgcaaaaaat gcgcttttta
      241 gccggtgatg aggtgaggcc gggaggggat gcgtcgccga tcacagttta caacagggtt
      301 tgataacggg aggaaaagtc atgctgcagg gattgaatca tctgacgctg gcggtcagcg
      361 atctggcgtc aagcctggca ttttatcagc agttacctgg aatgcgcctg cacgccagct
      421 gggatagcgg agcctatctc tcctgtgggg cgctgtggct gtgcttgtcg ctggatgagc
      481 agcggcgtaa aacgccccct caggaaagcg actataccca ctacgccttc agcgtggcgg
      541 aagaagagtt tgccggggtg gtggctctgc tggcgcaggc gggggctgag gtatggaaag
      601 ataaccgcag tgaaggggcg tcttactatt ttctcgaccc tgacggccat aagctggagc
      661 tgcatgtggg gaatctggcg cagcggctgg ccgcctgtcg cgaacgcccc tacaagggga
      721 tggtcttttt tgattgacgg gttagttcag cttactgccg gatttcaacg tgcagatcca
      781 cagcggtgaa ttgatagagc tgagcaagat agccttttcg ttgagcttct gagcgttgag
      841 aaacagcccg tcgtggctgt cggacatctc gcgcataaag tagtcgaaaa tcacgttcgg
      901 cgattcatca atcgaggagg cgctcgattc ccactgattg atgcggtaat gacgctcggt
      961 ggccgagatg cgtttgctgc tgtaggtaaa cttcacgtag cgttgcaggt agagccagcc
     1021 ggcggcggag tcggtgtaac cttcgaccac catcgagcct ttaccctgcg cgccaaaatt
     1081 gaagtgaata ttgccgttaa cgttctcttt gtccatgttc tcaaagcgca tgatgccctt
     1141 agtggaacag ctcatgacac ccgcgttgtc ggcaggcagc agccgccagg cgctaaacgc
     1201 ggcggcggca ataaatacca gaacgcagag ggcaaagaac ggacgcggcg cgagtctcat
     1261 tacggcttcc aggagtagta aaagtagtta tcgcaatagc tgaacgggtt atcttcatgc
     1321 atggcgcagt gggcaaggaa cacgcgcccc agcccgttgg tttcgagccg atcgccgtag
     1381 aagaaaagaa aacgttcacc gggtttacag gcaatattca gccgctggcg tacctcatcg
     1441 aagtttttgc cgtaggactc ttcactgacc gaacgtcgca tttcatcgct ggccagcagt
     1501 tcgcactggc tatggctgag gcgggtcagc gcgataggct gggattcact gatgccgacc
     1561 agactgaagg caaccagaag cagggcaatg gtgagcaagc aggctccggc catgtaccag
     1621 cacatcccgc gagcatgtgc ggagcgcggt gcgtctggcg ttgtcgtaac gggcggcggt
     1681 tcggcaggcg tctgttgtgg tgaaaccggc ggcgcgtcgt ccagcgcttc gataatgacc
     1741 tcgggattaa gctgcaaata gccgcgcgag acggtgacga tgatgttatc gacgccgtaa
     1801 tggcgaaagg tcttccgcag catgctgaga tactgattca gattgctgtt ggatgaggtt
     1861 aatccattgt catcccagac ttttttcaga acatcttccc ggctgacaat ctcggtatgg
     1921 cgcagaaaat agttaaacag ggcgctggcg gtgagggata gctggctgtc cggttcatcg
     1981 ctttgcggca gcgtcagggt tccgtcggtg gcgtcgtaga taaaacgggc attgatgttg
     2041 tagcgcatcg cggcctcagt ctgtgcttca cgcgggaagc gtcattttat tcagggagtc
     2101 aaccagcgcg cgacggtggt cggcgctcat ggtgatccct tccgtcaact ccgaaagcca
     2161 gatgccgtca gcggcgtagc gcaccagcgt gccggttggg ctgttatcca gctcatcgcc
     2221 ctgcgccagt ttctccagca tccagtcgcg ccagcatttg cgcagcaccg gttcatcggg
     2281 cattgccagc gacaaaacca taagctggcg gctttcatgg gtgtccgtga gatccgccag
     2341 atagttcaga taggcgcggg taaaccggcc atagctcacg ccatcctgtt gcatcagcgc
     2401 ggtaatcgct tcttccatga ttgccagcag acgcgcgaac agggcgaata ttagcgcctg
     2461 cttgttcggg aaatggtgga taaggccgcc ttt
//
LOCUS       DAEVZA010000289         2429 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEVZA010000289 DAEVZA010000000
VERSION     DAEVZA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.290"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..895
                     /locus_tag="LAF92_005150"
     CDS             <1..895
                     /locus_tag="LAF92_005150"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="catalyzes the phosphorylation of ribulose to
                     ribulose 5-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBI9794020.1"
                     /translation="GGAFDCHMGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAV
                     KGICGQVDGSVVPGFIGLEAGQSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQ
                     KQLLPALTEAWAKNPSLDHLPVVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGG
                     LIAATAFGARAIMECFTDQGIAVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQ
                     CCALGAAIFAAVAAKVHADIPSAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMS
                     AEQHYLPTSAPAQAAQAVATL"
     gene            906..2408
                     /gene="araA"
                     /locus_tag="LAF92_005151"
     CDS             906..2408
                     /gene="araA"
                     /locus_tag="LAF92_005151"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBI9794021.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
BASE COUNT          548 a          682 c          682 g          517 t
ORIGIN      
        1 cggcggcgcg tttgactgcc atatgggcgc agttggcgca ggcgcacagc ctaacgcact
       61 ggtaaaagtt atcggtactt ccacctgcga cattctgatt gccgacaaac agagcgttgg
      121 cgagcgggca gttaaaggta tttgcggtca ggttgatggc agcgtggtgc ctggatttat
      181 cggtctggaa gcaggccaat cggcgtttgg tgatatctac gcctggttcg gtcgcgtact
      241 cagctggccg ctggaacagc ttgccgccca gcatccggaa ctgaaagcgc aaatcaacgc
      301 cagccagaaa caactgcttc cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga
      361 tcacctgccg gtggtgctcg actggtttaa cggtcgtcgc acaccgaacg ctaaccaacg
      421 cctgaaaggg gtgattaccg atcttaacct cgctaccgac gctccgctgc tgttcggcgg
      481 tttgattgct gccaccgcct ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg
      541 gatcgccgtc aataacgtga tggcgctggg cggcatcgcg cggaaaaacc aggtcattat
      601 gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt gttgcctctg accagtgctg
      661 tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa gtgcacgcag acatcccatc
      721 agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg caaccgcgca gcgaacaggc
      781 acaacgcttt gaacagcttt atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca
      841 ctatcttcca acttccgccc cggcacaggc tgcccaggcc gttgcgactc tataaggaca
      901 cgataatgac gatttttgat aattatgaag tgtggtttgt cattggcagc cagcatctgt
      961 atggcccgga aaccctgcgt caggtcaccc aacatgccga gcacgttgtt aatgcgctga
     1021 atacggaagc gaaactgccc tgcaaactgg tgttgaaacc gctgggcacc acgccggatg
     1081 aaatcaccgc tatttgccgc gacgcgaatt acgacgatcg ttgcgctggt ctggtggtgt
     1141 ggctgcacac tttctccccg gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac
     1201 cgttgctgca attccacacc cagtttaacg cggcgctgcc gtgggacagt atcgatatgg
     1261 actttatgaa cctgaaccag actgcacatg gcggtcgcga gttcggcttc attggcgcgc
     1321 gtatgcgtca gcaacatgcc gtggttaccg gtcactggca ggataaacaa gcccatgagc
     1381 gtatcggctc ctggatgcgc caggcggtct ctaaacagga tacccgtcat ctgaaagtct
     1441 gccgttttgg cgataacatg cgtgaagtag cggtcaccga tggtgataaa gttgccgcac
     1501 agatcaagtt cggtttctcc gtcaatacct gggcggttgg cgatctggtg caggtggtga
     1561 actccatcag cgacggcgat gttaacgcgc tggtcgatga gtacgaaagc tgctacacca
     1621 tgacgcctgc cacacaaatc cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta
     1681 ttgagctggg gatgaagcgt ttcctggaac aaggtggctt ccacgcgttc accaccacct
     1741 ttgaagattt gcacggtctg aaacagcttc caggtctggc cgtacagcgt ctgatgcagc
     1801 agggttacgg ctttgcgggc gaaggcgact ggaaaaccgc cgccctgctt cgcatcatga
     1861 aggtgatgtc aaccggtctg cagggcggca cctcctttat ggaggactac acctatcact
     1921 tcgagaaagg taatgacctg gtgctcggct cccatatgct ggaagtctgc ccgtcgattg
     1981 ccgtagaaga gaaaccgatc ctcgacgttc agcatctcgg tattggtggt aaggacgatc
     2041 ctgcccgact gatcttcaat acccaaaccg gtccagcgat tgtcgccagc ctgattgatc
     2101 tcggcgatcg ttaccgtctg ctggttaact gtatcgacac ggtgaaaaca ccgcactccc
     2161 tgccgaaact gccggtggcg aatgcgctgt ggaaagcgca accggatctg ccaactgctt
     2221 ccgaagcgtg gatccttgct ggtggcgcgc accataccgt cttcagccat gcgctgaacc
     2281 tcaacgatat gcgccagttc gccgagatgc acgacattga aatcacggtg attgataacg
     2341 acacccgcct gccagcgttt aaagacgcac tgcgctggaa cgaagtgtat tacggatttc
     2401 gtcgctaagt agccacatca ggtatgtaa
//
LOCUS       DAEVZA010000290         2357 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEVZA010000290 DAEVZA010000000
VERSION     DAEVZA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.291"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            630..2297
                     /gene="ltrA"
                     /locus_tag="LAF92_005152"
     CDS             630..2297
                     /gene="ltrA"
                     /locus_tag="LAF92_005152"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBI9794022.1"
                     /translation="MNTHISVSTIPHPTCWHTINWKACHARVRKLQLRIAKATRQQQW
                     RQVRELQRILTRSFSGKAVAVRRVTENTGKRTPGIDGKIWHTPKEKWEGICSLNLCGY
                     RPQPLRRIHIPKSNGKTRPLGIPTMRDRAMQALWLLALEPVSETTADHNSYGFRPMRS
                     THDAIESIFLRMSQKVSPKWILEGDIKGCFDNISHDWLLSHIPMDRRLLKKWLKAGYM
                     ERGVFNHTNSGTPQGGIISPVLANMALDGLEKELMQTFRKSGYHSAKHQVNYVRYADD
                     FICSGSSRELLENEVRPLIAAFMRERGLELSEEKTAITHIDKGFDFLGQNVRKYNGKM
                     LIKPSKKNLKNFLCKVREIIKRNPTLPAWKLIGQLNPVIRGWATYHRHVVAKETFNYV
                     DTQIWRAIWRWCVRRHPRKGLRWIAGRYFSFEGRRWIFKAITPEGKILTLFRAMETPI
                     KRHIKIKGEATPYTPGMEIYFERRLDLIWKGKSKKMKTVVQLWKRQGKHCPQCGQLIT
                     NQTGWNIHHRIRKVMGGSDELTNLELLHPNCHRQLHSREAGAHRKHL"
BASE COUNT          692 a          584 c          652 g          429 t
ORIGIN      
        1 ggatgccgcc aggcagccgg gagccgaagg tgcgcgtgcg acaggaagtc gcttaacata
       61 ttgttttaca ttgataaaac cagctatccc attagctgta gcctaccggg acaggcgttg
      121 ccggcaacgg cggggtctga agctcccggg acaacgagag ccgggcgtca gcccgggtaa
      181 agccccgtga ggggctttac cttgctgcaa gcatcatgcg gcaggagagc aaggctggtc
      241 agaagggtta acacatgtga actgttgata aacaccgtta tcggcaacag gccaaagatg
      301 ctgacaggcc tgaaccaaaa ggtacggggt ccggttgcta cctcgcgcac agggagcaca
      361 tgggcgcaga gacccccggt gaagagacag aacccgccct gaccctctgt atgttaagcg
      421 gaacctggta aacccgtatc gctgcctcac aggcaggtga accgcaagga acactgtcag
      481 cggtgcgggc agaggaggac ggaaaaagcg aatgcccggc tgtaataacc gggatacggg
      541 gcaggttccc cgacgcgaaa gcgggcagac ttccgtctgg tctttaattg catgaaaagc
      601 tgtaaagcag tcaaaaggat atcagcgaga tgaatacaca catcagtgtg tccacgatac
      661 cccacccgac atgctggcac accataaact ggaaagcatg ccatgcccgg gtgcgaaagc
      721 tacagttacg gattgcaaag gcaacccgac agcaacaatg gcggcaggtc agagaactac
      781 agcgcatcct cacccgctca ttctccggca aagccgtggc tgtcaggcgt gtaactgaaa
      841 ataccggtaa aaggacaccc ggtattgacg gaaaaatatg gcacacaccg aaggagaaat
      901 gggaaggaat atgcagcctg aatctgtgtg gttacaggcc acagccacta cggcgaatac
      961 atatcccaaa atcaaacggc aaaacgcgac cgctgggcat acccacaatg agggaccggg
     1021 ccatgcaggc actgtggctg ctggctctgg aacccgtatc cgagaccact gctgaccata
     1081 acagctatgg tttcagacca atgcgcagca cgcatgacgc cattgagtcc atttttctgc
     1141 ggatgagcca gaaagtatcg cccaaatgga tactggaggg cgatattaaa gggtgcttcg
     1201 ataacatcag ccatgactgg ctgctttccc atattccgat ggacaggaga ctgcttaaaa
     1261 aatggttgaa agcaggttac atggaaagag gcgtgtttaa ccatacgaac agcgggacac
     1321 cgcagggagg catcatatcc cctgttctcg ccaacatggc tctggacgga ctggagaagg
     1381 aactgatgca aacgttcagg aaaagtggtt atcacagtgc aaagcaccag gtgaattatg
     1441 tgagatatgc tgatgatttc atctgttcag gcagctcccg tgaattgctg gaaaatgagg
     1501 tcagaccact gattgcagct ttcatgcgcg aacgagggct ggaactgtcg gaagaaaaaa
     1561 cagcgatcac acatatagat aaaggcttcg actttttagg gcagaacgtc aggaagtaca
     1621 acggtaaaat gctgattaaa ccgtcgaaga aaaacctgaa gaacttcctc tgtaaagtca
     1681 gagaaataat taaacgcaat ccgacgctcc cggcatggaa actgataggc cagctaaacc
     1741 cggtgatccg gggatgggct acctatcacc gacacgtcgt ggcgaaagaa acatttaatt
     1801 atgtcgacac ccaaatctgg cgagctatat ggcgatggtg tgtaagacgg catccccgga
     1861 aaggattgcg gtggatcgcc ggacgatact tctcctttga aggcaggcgc tggatcttta
     1921 aagccataac cccggaaggg aaaattctga cactcttccg ggcaatggaa acaccgataa
     1981 agcgccatat aaaaatcaaa ggcgaagcaa caccatacac ccccggaatg gaaatctact
     2041 ttgaacgtcg tctggatcta atctggaaag gcaaatcaaa gaagatgaag acagtagttc
     2101 aactctggaa gaggcagggg aaacactgtc cgcagtgcgg acaactcata accaaccaga
     2161 ccggatggaa tatacatcac cggatcagga aagttatggg cggcagtgac gaactgacca
     2221 atctcgagtt gttacatccg aactgccacc gacagttaca cagcagggaa gccggtgctc
     2281 accgaaagca cttataaagg cttgagcgga gtgcggggaa actcgcacgc tccgttctta
     2341 gggggcggcg gcgcagc
//
LOCUS       DAEVZA010000291         2267 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEVZA010000291 DAEVZA010000000
VERSION     DAEVZA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.292"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..458)
                     /locus_tag="LAF92_005153"
     CDS             complement(<1..458)
                     /locus_tag="LAF92_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBI9794023.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSAR"
     gene            complement(523..1122)
                     /locus_tag="LAF92_005154"
     CDS             complement(523..1122)
                     /locus_tag="LAF92_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HBI9794024.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(1192..>2267)
                     /locus_tag="LAF92_005155"
     CDS             complement(1192..>2267)
                     /locus_tag="LAF92_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HBI9794025.1"
                     /translation="DDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSIT
                     DVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGA
                     GIENTPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITS
                     GGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFV
                     KAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYSDPGSGNN
                     PGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKIFQKGNQ
                     GAGNITDCTVIVTKKAASGISIR"
BASE COUNT          493 a          661 c          522 g          591 t
ORIGIN      
        1 cgtgctgagc ctgcagcatc agtcgcatgg gttgccgcct cacgggctga tgtgctggca
       61 tcgctggctg acttcttcgc ggctgccgtg ttctgtgcca ccgcggacgc gttacgcgcc
      121 acctcttcca ccatcagttc aaaacggcgc agtgcctcag gacgggcatc atcctccgtc
      181 atggcaccga gaaaatcatt cagcgtaccg ggtcgggaat cttcatacac ggtgatggtc
      241 ccggcatgtg acggcgggaa tccctccacc aacagaataa cgctgtactg cccgtactca
      301 acgtccatgc tgtaacgccc ggcttcatcc ggattttctg aggccagcgt gttcaccacc
      361 accgtggtgc tgttacgttt tgctttcagc tggattgtgc agttctgtac cggttttcct
      421 gtgccgtctt tcagtacacc tgaaatcttt actgccatat tcaccccaca aaaaagcccg
      481 cctgaaccgg cgggctgtca taacactgtg ttacctggct aatcagaatt tataaccgac
      541 acccacgatg aaaccgtcag tgcgccagtc accactgccg gagccttcat aagcaatatc
      601 aatggccacg gattcggtcg ggttaaactg cacgccagcc ccccacgcca gagacgtgtt
      661 gctgtggcga ccgtcatcac ttccggtcag cacatcgtgc tttttcccct tgttgtcagt
      721 tacgcggaga taatccccgg agaaagtcga cacacggctg taagccacac ccgccatcgc
      781 atacgcgctg aaccattcat tcacgcgcac agacggcccc gccattacgc tgaaccagcg
      841 gttacgaacg gaatcttcat gccagcgggt atcgctgtaa cgggtcagct ggcgattctt
      901 gtctcctgca tagctgaatg acgtcaccag ccccagtgtg tccgtaaact cataacggta
      961 tttcacgtta atcccgttca gatcatcact gccggggacg ttcgtcgagg catgaagata
     1021 ccccgcgctc agcgtggact gatgttcaga cgcccatgca ggcgcaccgg atacggccag
     1081 acaaatggct gcggacaaaa ttgctgcaca aactttacgc ataattacct ctcgcttttc
     1141 tgcaataaaa aaggcgtcat tcctgacgcc ctttattggg ttataaatat ttcaacgaat
     1201 actgatgccg gaagcggctt ttttggtcac aatcaccgta cagtcggtga tattgcctgc
     1261 cccctgattg cctttctgga aaatcttaaa ctccagagtg acgcttcccc tgccactcgg
     1321 catatcaata actgcactgt aactaccggg aatggcccct ttagtttctc tggatgcgat
     1381 taatacgccg tttttgcgaa cttcaaaacc ataacccgtg tatcttgtac ctcccgggtt
     1441 attaccactt cccggatcgc tatacgctat tccgttaaag ataatgggcg gaataatgat
     1501 ttgacggtca aagttatgat catcgctgat ggtgactgta accgtcccgt ttggtgtttc
     1561 cgtattaccc cacgtaccag cctgtttcgg gaatgatttg gatacagctt taacgaagtc
     1621 acctctgacc tgagtcgcct ccagcatgcc cttaatcgta cagttttcat ttaccgtgac
     1681 attgttgagc gtcccggcgt tcgcattcac actgccactg atatccgcat ttttagcggt
     1741 cagctttccg tccggtgtca gggaaaatgc cggcggattt ccaccgctgg taatggtggg
     1801 agccgtcagg cgtttcagga acacgtcatt catgaatatc tgatcaccct gaccaacaaa
     1861 catcggtttt gtgttgccat ttgcaggatt aatcatcgca atcctgtccg ccgccagcag
     1921 cacctgactc tgcatgccgt caggggtgtt ctcaataccg gcaccgatac ccgcaatata
     1981 aaggcgtccg tcctgcatct gctgcagctt cactgcccac atgctgttca ggttattatt
     2041 tgtatcaacc tgaaccttct gtatctgctg gatcgctgca ctctggtctt ccagtttctt
     2101 attgacggtc tgtgttattt cattgctgac atccgttatg gacgtcctga tttcagtcag
     2161 gtcaggcgca agctgaccgt tatcaatctg cgtccacagc tcctgagcca gatgggtttt
     2221 ccctatctcg cctttgaaaa aatccagata gcctgatgca tcatcac
//
LOCUS       DAEVZA010000292         2243 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEVZA010000292 DAEVZA010000000
VERSION     DAEVZA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.293"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1048
                     /locus_tag="LAF92_005156"
     CDS             <1..1048
                     /locus_tag="LAF92_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HBI9794026.1"
                     /translation="SGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVS
                     NEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIE
                     NTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKRLTAPTITSGGN
                     PPAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTV
                     GKAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRL
                     IVKKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDWYPTASISDLL
                     VVVMKKATAGISIS"
     gene            1116..1715
                     /locus_tag="LAF92_005157"
     CDS             1116..1715
                     /locus_tag="LAF92_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBI9794027.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTNSFIVGVGYKF"
     gene            1780..>2243
                     /locus_tag="LAF92_005158"
     CDS             1780..>2243
                     /locus_tag="LAF92_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBI9794028.1"
                     /translation="MAVRISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENPD
                     EAGRYTMDVEYGQYSVSLLVEGFPPSHAGIITVYEDSKPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAA"
BASE COUNT          568 a          527 c          661 g          487 t
ORIGIN      
        1 atcaggctat ctggattttt tcaaaggcga gatagggaaa acccatctgg ctcaggagct
       61 gtggacgcag attgataacg gtcagcttgc gcctgacctg gctgaaatca ggacgtccat
      121 tacggatgtc agcaatgaaa tcacacagac cgtcaataag aaactggaag accagagtgc
      181 agcgatccag cagatacaga aggttcaggt tgatacaaat aataatctga acagcatgtg
      241 ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat attgcgggta tcggtgccgg
      301 tattgagaat acgccagcag gaatgcagag tcaggtgctg ctggcggcag acaggattgc
      361 gatgattaat cctgcgaatg gcaacacaaa accgatgttt gttggtcagg gtgatcagat
      421 attcatgaat gaagtgttcc tgaagcgcct gacggccccc accattacca gcggcggcaa
      481 tcctccggcc ttttccctga caccggacgg aaagctgacc gctaaaaatg cggatatcag
      541 tggcagtgtg aatgcgaact ccgggacgct caacaacgtt acgataaatg agaactgtca
      601 gattaaaggg aaactgtcag ccaaccagat tgaaggcgat attgtcaaaa cagtgggtaa
      661 ggctttcccg cgggactccc gtgcaccgga gcggtggcca tcaggaacca ttaccgtcag
      721 gatttatgac gatcagccgt ttgaccggca gattgttatt ccggcggtgg cattcagcgg
      781 tgctaaacat gagagagagc atactgatat ttactcctca tgccgcctga tagtgaagaa
      841 aaatggtgct gaaatttata accgtaccgc gctggataat acgctgattt acagtggtgt
      901 tattgatatg ccagctggtc acggccacat gacgctggag ttttcggtgt cagcatggct
      961 ggtaaatgac tggtatccca cagcaagtat cagcgatttg ctggttgtgg tgatgaagaa
     1021 agccaccgca ggcatcagta tcagctgaat tttataaccc atatacgggc gccagaaatg
     1081 gcgccttttt tattgcagaa aagcgagagg taattatgcg taaagtttgt gcagccattt
     1141 tgtccgcagc catctgtctg gccgtatccg gtgcgcctgc atgggcgtct gagcagcagg
     1201 ccacactgag cgcagggtat cttcatgccc gtacgaacgc tcccggcagc gataatctga
     1261 acgggattaa cgtgaaatac cgttatgagt ttacggacac gctggggctg attacgtcat
     1321 tcagttatgc caacgctgaa gatgagcaaa aaacgcatta cagcgatacc cgctggcatg
     1381 aggattccgt gcgtaatcgc tggttcagcg tgatggcggg gccgtctgtg cgcgtgaatg
     1441 aatggttcag cgcgtatgcg atggcgggta tggcttacag ccgtgtgtcg actttctccg
     1501 gggattatat ccgcgtaact gacaacaagg ggaaaacgca cgatgtgctg accggaagtg
     1561 atgacggtcg ccacagcaac acgtctctgg cgtggggagc tggcgtgcag tttaacccga
     1621 ccgaatccgt ggccgttgac gtcgcttatg aaggctccgg cagcggtgac tggcgtacca
     1681 acagtttcat cgttggtgtc ggttataaat tctgattagc caggtaacac agtgttatga
     1741 cagcccgccg gttcaggcgg gcttttttgt ggggggaata tggcagtacg gatttcaggt
     1801 gtactgaaag atggcgcagg taagccgata caaaactgca ccattcagct aaaggccagg
     1861 cgcaacagca ccacggtggt ggtgaacaca gtggcctcag aaaacccgga tgaagccggg
     1921 cgttacacaa tggacgtcga gtatggtcag tacagcgtca gtctgttggt ggagggattc
     1981 ccgccgtcac acgccgggat tatcaccgta tatgaggact caaagccggg cacactgaat
     2041 gatttcctcg gtgcaatgac ggaggatgat gtccggccag aggcactgcg ccgttttgaa
     2101 ctgatggtgg aagaggtggc gcgtaacgcg tccgcagtgg cacagaacac ggcagccgcg
     2161 aagaagtcag ccggcgatgc cggcacatct gcccgtgagg cggcaaccca tgcgactgat
     2221 gctgcaggct cagcacgcgc agc
//
LOCUS       DAEVZA010000293         2225 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEVZA010000293 DAEVZA010000000
VERSION     DAEVZA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.294"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1104
                     /locus_tag="LAF92_005159"
     CDS             <1..1104
                     /locus_tag="LAF92_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI9794029.1"
                     /translation="DGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQCRS
                     LAEKIQQEGSEDGHGVVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTVERLAA
                     QIEPVYLPERKIHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPY
                     RLPGQQYDKESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGL
                     DAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYL
                     MCRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDAC
                     IEKYNTGQLFGLDGIKADNPIKTKQGSSDASNY"
     gene            1088..1570
                     /locus_tag="LAF92_005160"
     CDS             1088..1570
                     /locus_tag="LAF92_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794030.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGHKTPKSV
                     EIYLSEKNIIYKIINDQKISRGNGHFISIMVNNYRTHCGVVDINLNFFNDILYSVRLK
                     NISKLENMEFCAKKQRVYFSDKNKKASYKIINYGDYYDVDYYDNNLKNEVFDWIGKWS
                     "
     gene            complement(1485..1670)
                     /locus_tag="LAF92_005161"
     CDS             complement(1485..1670)
                     /locus_tag="LAF92_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794031.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHRNSLHN"
     gene            1751..>2225
                     /locus_tag="LAF92_005162"
     CDS             1751..>2225
                     /locus_tag="LAF92_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBI9794032.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSAILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKMFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPEL"
BASE COUNT          710 a          374 c          576 g          565 t
ORIGIN      
        1 gacggcgaca ggctgacgac ggtacagacc gacaccacgc gtatccagac ggtataccag
       61 ccggggagct tcgcgccgct catccgcatc gaaacagaca acggtgaacg ggagaaagcg
      121 cagtgccgca gcctggcgga gaagatacag caggaaggga gcgaggacgg gcacggcgtg
      181 gtatttccgg ctgaactggt ggggctgctg gacaggctgg agggggaaat ccgggcaaac
      241 tgcgtgagca gtgaaagccg ccagtggctt gcgcagtgcg ggctgacggt ggagcggctg
      301 gcggcacaga tagagccggt ttatttgcct gagcgaaaaa tacatcttta tcactgtgac
      361 caccggggcc tgccgctggc gcttatcagt gaagacggca atacggcgtg gagcgcggaa
      421 tatgatgaat ggggcaacca gcttaatgag gagaacccgc atcacctgca ccagccgtac
      481 cggctgccgg ggcagcagta tgataaggag tcggggctgt actataaccg tcaccggtac
      541 tacgatccgt tgcaggggcg gtatatcacc ccggacccga ttgggttgag aggtggatgg
      601 aatatgtatc agtatccgtt gaatcccata caagtgatag acccaatggg gttagatgcg
      661 attgagaata tgacatcagg tggactaatt tatgccgtat ctggtgtacc tggattgatt
      721 gctgcaaaca gcattactaa cagtgcttac cagttcggtt atgatatgga tgctattgtt
      781 ggcggagctc ataatggggc cgccgatgca atgagacatt gttacttgat gtgtcgaatg
      841 actaagacat ttggatcaac aatagctgac gtgataggta aaaatcatga ggcggcaggg
      901 gatagacaag gtcagccagc taaagaaaga atcatggatc ttaaaaataa cactgtcggt
      961 attgcttgtg gcgatttttc tgccaaatgt agcgatgcat gtattgaaaa atataacact
     1021 gggcaactct tcgggttaga tggtataaaa gcagataatc caataaaaac aaagcaaggg
     1081 agttcagatg cttcaaatta ttagaggcaa acttgtcatt tttttaatta ccctttgttt
     1141 atttgttgtt taccttgggt ttgataacaa ttcaaattct gacatcgtat tttatggaca
     1201 taaaacacca aagagcgttg agatatatct ttctgaaaaa aatattattt ataaaataat
     1261 taatgaccaa aaaattagta gagggaatgg tcattttata agtataatgg ttaataatta
     1321 caggacgcac tgtggagtgg ttgatataaa tcttaatttt ttcaatgata ttctttatag
     1381 cgtgcgatta aaaaacatta gtaaactgga gaatatggag ttctgcgcta aaaaacaacg
     1441 ggtgtatttt agtgataaaa acaagaaggc tagttataaa ataattaatt atggagacta
     1501 ttacgatgtt gattattatg ataacaattt gaaaaatgaa gtttttgact ggattggtaa
     1561 atggtcataa tgaacaacac cagaaagaga aaaatataaa atacatacag aaagacatga
     1621 gagcaaggga cggggcaggg caagatcatg aaaattgtga tgtaaatcac gattttcatc
     1681 ttggctttaa cgcctacagg tgatcaaaat ttaacctttg gaataactaa aaagataaaa
     1741 aaggacccag gtgagtattc aaagtttgct tgattatatt tcagtgatcc ctgatatacg
     1801 acaacaagga aaggttaaac ataaattatc tgctattttg tttctcaccg tatgtgcagt
     1861 aattgcaggt gccgatgagt ggcaggaaat tgaagatttt ggacatgaaa gacttgaatg
     1921 gctaaagaaa tatggtgatt ttgataatgg cattccggtc gatgacacca ttgcacgcgt
     1981 tgtgagtaac attgacagtt tggcctttga aaagatgttt attgaatgga tgcaggagtg
     2041 ccatgaaatc actgatggtg aaattatagc aatagatgga aagaccataa gaggctcctt
     2101 tgataaggga aaaagaaaag gagcaatcca tatggtgagt gcattctcga acgaaaatgg
     2161 tgttgtactg gggcaggtga aaacggaagc caaaagtaat gagattacag ccattccaga
     2221 gttgc
//
LOCUS       DAEVZA010000294         2123 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEVZA010000294 DAEVZA010000000
VERSION     DAEVZA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.295"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>2123
                     /gene="traC"
                     /locus_tag="LAF92_005163"
     CDS             <1..>2123
                     /gene="traC"
                     /locus_tag="LAF92_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBI9794033.1"
                     /translation="SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLP
                     EGMNLPLTLRHYRVFFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQA
                     FIDIVGEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNS
                     TARILNFHLARNPEIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEAN
                     LKYMDLEKKSKTSYAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNE
                     TALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAES
                     FNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTG
                     LIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAE
                     RVRDQLSVMASPNGNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYV
                     ESPTIRSRLDEMIVLLDQYTANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLV
                     AVMFSLIIYIENRMYRTPRNLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGA
                     YITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDM
                     IGKFGAAKDQWFSSFLLQVENHSSW"
BASE COUNT          505 a          547 c          590 g          481 t
ORIGIN      
        1 ccagtcagct tgttggtgac aggattgaat acgggctgcg tgagttctcc tggtcaggtg
       61 aacaggctga acggtttaac gccattaccc gtgcctatta tatgaacgcg gcagcgacac
      121 agtttccgct gccggagggg atgaatctgc ccctgaccct gcgccattac cgggtattct
      181 tctcgtactg ttctccctcg aagaaaaaaa gccgggctga cattctggaa atggaaaacc
      241 tggtgaaaat catccgggcg tcgttacagg gggccagtat caccacacag gcggtggatg
      301 cacaggcctt tatcgatatt gtcggggaga tgattaacca taacccggac tccctgtacc
      361 cgaaaagacg tcagctggac ccgtattctg atctgaatta tcagtgtgtg gaggacagtt
      421 ttgatctgaa ggtccgggcg gattacctga cgctggggct gcgtgagaac ggcaggaaca
      481 gcacagcccg tatcctgaat ttccatctgg cccgtaaccc ggaaatcgcc ttcctgtgga
      541 acatggccga taactacagc aatctgctga acccggaaat gtccatttcc tgcccgttca
      601 tcctgacgct gacactggtg gtggaagatc aggtgaaaac ccacagtgaa gccaacctga
      661 agtacatgga cctggagaaa aagtcgaaga cctcctatgc caaatggttt ccgtccgtgg
      721 agaaagaagc taaggagtgg ggggaactgc gccagcggct gggctccggt cagtcttctg
      781 tcgtgtccta cttccttaac atcacagcat tctgcaagga taacaatgaa acggctctgg
      841 aagtggagca ggacattctg aacagctttc gtaaaaacgg ttttgagctg atttcaccgc
      901 gctttaacca catgcgcaat ttcctgacct gcctgccctt tatggccggg aaagggctgt
      961 ttaaacagct gaaagaggcc ggtgtggttc agcgcgcaga gagctttaat gtggccaacc
     1021 tgatgccgtt agtggcggat aaccccctga caccggcagg cctgctggca cccacctacc
     1081 gtaaccagct ggcgtttatc gatatcttct ttaaggggat gaataacacc aactacaaca
     1141 tggcggtctg tggaacatcc ggggccggta aaaccgggtt gatacagcca cttatccgca
     1201 gcgtgctgga ctccgggggc tttgccgtgg tgttcgacat gggggatggc tacaagtctt
     1261 tgtgtgagaa catggggggc gtatatctgg acggtgaaac cctgcgcttt aacccgtttg
     1321 cgaacatcac tgatattgac cagtcagcgg agcgtgtccg tgaccagttg tcggtgatgg
     1381 ccagccccaa cggtaacctg gatgaagtgc atgaaggtct gttgctgcag gcggtcagag
     1441 cctcctggct ggctaaaaag aagcaggcgc gcattgatga tgtggtggat ttcctgaaaa
     1501 acgcccgtga taacgaccag tatgtcgagt caccgaccat ccgcagccgt ctggacgaaa
     1561 tgattgtgct gcttgaccag tacactgcca acggcactta cggccggtat tttaactctg
     1621 atgaaccgtc cctgcgggat gatgccagaa tggtggtgct ggagctgggt gggctggaag
     1681 accgtccgtc actgctggtt gcggtgatgt tcagccttat catttacatc gagaaccgga
     1741 tgtaccgcac gccgcgtaac ctcaagaaac tgaacgtcat tgatgaaggc tggcgtctgc
     1801 tggacttcaa aaatcgtaaa gttggtgagt ttattcaaaa aggttaccgc acctgtcgcc
     1861 gtcataccgg tgcctatatc accatcacac agaacatcgt cgactttgac tctgacaagg
     1921 catccagcgc agcccgcgcg gcatggggta actcctccta caaaattatc ctcaaacaga
     1981 gtgcgaagga gttcgcgaaa tacaaccagc tgtatccgga ccagttcctg ccactgcagc
     2041 gcgacatgat tggtaagttt ggtgcggcca aagaccagtg gttcagttcc ttcctgctgc
     2101 aggtggagaa ccactcctcc tgg
//
LOCUS       DAEVZA010000295         2101 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEVZA010000295 DAEVZA010000000
VERSION     DAEVZA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.296"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(60..1025)
                     /locus_tag="LAF92_005164"
     CDS             complement(60..1025)
                     /locus_tag="LAF92_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBI9794034.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1891..>2101)
                     /locus_tag="LAF92_005165"
     CDS             complement(1891..>2101)
                     /locus_tag="LAF92_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794035.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          581 a          551 c          440 g          529 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaaa cccccaaaac aagccccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
     1861 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
     1921 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
     1981 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     2041 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     2101 t
//
LOCUS       DAEVZA010000296         2059 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEVZA010000296 DAEVZA010000000
VERSION     DAEVZA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.298"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            228..1019
                     /locus_tag="LAF92_005166"
     CDS             228..1019
                     /locus_tag="LAF92_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HBI9794036.1"
                     /translation="MRVAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQ
                     DLFEALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
     gene            1151..1972
                     /gene="lnu(F)"
                     /locus_tag="LAF92_005167"
     CDS             1151..1972
                     /gene="lnu(F)"
                     /locus_tag="LAF92_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(F)"
                     /protein_id="HBI9794037.1"
                     /translation="MLQLKMIELFKEGCHEDARIIAALMFGSFAIGEGDEFSDIEFAV
                     FIQDDHFENFDQRSWLNAVSPVAAYFPDDFGHHTALFENGIRGEFHFMRKSDIPVIST
                     WQGYGWFPSLEAAVLLDRSGELSRYASALVGGPPIREGAPLVEGLVLNLISLMLFGAN
                     LLNRGEYARAWALLSKAHENLLKLVRLHEGATDHWPTPSRALEKDISEDSYNRYLACT
                     SSAEPRALCAAYHQTWTWSLELFKSVTEPLNIELPRTVIAQAKRLLNESATPHNK"
BASE COUNT          520 a          502 c          547 g          490 t
ORIGIN      
        1 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
       61 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
      121 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
      181 acgatgttac gcagcagggc agtcgcccta aaacaaagtt agacatcatg agggtagcgg
      241 tgaccatcga aatttcgaac caactatcag aggtgctaag cgtcattgag cgccatctgg
      301 aatcaacgtt gctggccgtg catttgtacg gctccgcagt ggatggcggc ctgaagccat
      361 acagcgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
      421 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
      481 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
      541 gcgaactgca atttggagaa tggcagcgca atgacattct tgcgggtatc ttcgagccag
      601 ccatgatcga cattgatcta gctatcctgc ttacaaaagc aagagaacat agcgttgcct
      661 tggtaggtcc ggcagcggag gaattctttg acccggttcc tgaacaggat ctattcgagg
      721 cgctgaggga aaccttgaag ctatggaact cgcagcccga ctgggccggc gatgagcgaa
      781 atgtagtgct tacgttgtcc cgcatttggt acagcgcaat aaccggcaaa atcgcgccga
      841 aggatgtcgc tgccgactgg gcaataaaac gcctacctgc ccagtatcag cccgtcttac
      901 ttgaagctaa gcaagcttat ctgggacaaa aagaagatca cttggcctca cgcgcagatc
      961 acttggaaga atttattcgc tttgtgaaag gcgagatcat caagtcagtt ggtaaatgat
     1021 gtctaacaat tcgttcaagc cgaccgcgct acgcgcggcg gcttaactcc ggcgttgtgc
     1081 tgacgaaaaa aaacaaaaat ctgcgtatac ctttcctcat aatctgtggc ttcaataaaa
     1141 ggatatttct atgcttcaac tgaaaatgat cgaactcttc aaggaaggtt gtcatgagga
     1201 tgcacgaata atcgcggcat tgatgttcgg ctcatttgct atcggagagg gtgacgagtt
     1261 ctctgatatc gaattcgcag tgttcatcca ggatgaccat tttgaaaatt tcgatcagcg
     1321 ctcgtggctt aatgccgtaa gtccggttgc tgcttacttt ccggacgact tcggccacca
     1381 caccgcactt tttgaaaacg gcattcgcgg tgaattccat ttcatgcgaa aatcggacat
     1441 accggtcatt tccacttggc aaggctatgg gtggtttccc tcgcttgagg cggctgtttt
     1501 gttggaccga tcaggagagt tgtcaaggta cgcaagcgct ctcgtgggcg gtcccccgat
     1561 acgtgaaggc gcgccgctgg tggaagggct tgtgttgaac ctcatcagcc tgatgctctt
     1621 tggggccaat cttttaaatc ggggagagta cgctcgcgcc tgggctttgc tcagcaaagc
     1681 acatgaaaac ctactcaagc tggttcgact ccacgaaggg gcaacagacc actggccgac
     1741 accttcacgc gcgctcgaaa aggatatctc ggaggactcg tataatcgct atctggcatg
     1801 cacaagcagt gcagaaccaa gagcactatg tgcagcctat catcaaacgt ggacgtggag
     1861 tctcgaattg ttcaagagcg tgacagaacc tctgaatatc gagcttccga gaactgtaat
     1921 tgcgcaggca aaaaggttgc tcaatgagtc tgcgacgccg cacaacaagt aaatccagcg
     1981 gacgcataaa aacgcgccgc tgattttgac gttagatgca ctaagcacat aattgctcac
     2041 agccaaacta tcaggtcaa
//
LOCUS       DAEVZA010000297         2048 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEVZA010000297 DAEVZA010000000
VERSION     DAEVZA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.299"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..635
                     /gene="ispD"
                     /locus_tag="LAF92_005168"
     CDS             <1..635
                     /gene="ispD"
                     /locus_tag="LAF92_005168"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBI9794038.1"
                     /translation="KQYLSIGNQTILEHSVHALLAHPRVKRVVIAISPGDSRFAQLPL
                     ANHPQITVVDGGDERADSVLAGLKAAGDAQWVLVHDAARPCLHQDDLARLLALSETSR
                     TGGILAAPVRDTMKRAEPGKNAIAHTVDRNGLWHALTPQFFPRELLHDCLTRALNEGA
                     TITDEASALEYCGFHPQLVEGRADNIKVTRPEDLALAEFYLTRTIHQENT"
     gene            635..1114
                     /gene="ispF"
                     /locus_tag="LAF92_005169"
     CDS             635..1114
                     /gene="ispF"
                     /locus_tag="LAF92_005169"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBI9794039.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            1111..>2048
                     /gene="truD"
                     /locus_tag="LAF92_005170"
     CDS             1111..>2048
                     /gene="truD"
                     /locus_tag="LAF92_005170"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBI9794040.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNW"
BASE COUNT          483 a          505 c          597 g          463 t
ORIGIN      
        1 ctaagcaata tctctcaatc ggtaatcaaa ccattcttga acactcggtg catgcgctgc
       61 tggcgcatcc ccgggtgaaa cgtgtcgtca ttgccataag tcctggcgat agccgttttg
      121 cacaacttcc tctggcgaat catccgcaaa tcaccgttgt agatggcggt gatgagcgtg
      181 ccgattccgt gctggcaggt ttgaaagccg ctggcgacgc gcagtgggta ttggtgcatg
      241 acgccgctcg tccttgtctg catcaggatg acctcgcgcg attgttggcg ttgagcgaaa
      301 ccagccgcac gggagggatc ctagccgcac cagtgcgcga tacgatgaaa cgtgccgaac
      361 cgggcaaaaa tgccattgct cataccgttg atcgcaacgg cttatggcac gcgctgacgc
      421 cgcaattttt ccctcgtgag ctgttacatg actgtctgac gcgcgctcta aatgaaggcg
      481 cgactattac cgacgaagcc tcggcgctgg aatattgcgg attccatcct cagttggtcg
      541 aaggccgtgc ggataacatt aaagtcacgc gcccggaaga tttggcactg gccgagtttt
      601 acctcacccg aaccatccat caggagaata cataatgcga attggacacg gttttgacgt
      661 acatgccttt ggcggtgaag gcccaattat cattggtggc gtacgcattc cttacgaaaa
      721 aggattgctg gcgcattctg atggcgacgt ggcgctccat gcgttgaccg atgcattgct
      781 tggcgcggcg gcgctggggg atatcggcaa gctgttcccg gataccgatc cggcatttaa
      841 aggtgccgac agccgcgagc tgctacgcga agcctggcgt cgtattcagg cgaagggtta
      901 taccctgggc aacgtcgatg tcactatcat cgctcaggca ccgaagatgt tgccgcacat
      961 tccacaaatg cgcgtgttta ttgccgaaga tctcggctgc catatggatg atgttaacgt
     1021 gaaagccact actacggaaa aactgggatt taccggacgt ggggaaggga ttgcctgtga
     1081 agcggtggcg ctactcatta aggcaacaaa atgattgagt ttgataatct cacttacctc
     1141 cacggtaaac cgcaaggcac cgggctgctg aaagccaatc cggaagactt tgtggtggtg
     1201 gaagatttgg gctttgagcc tgatggtgaa ggtgagcata ttctggttag aatcctcaaa
     1261 aacggctgca atacccgttt tgtggcggat gcactggcga aattcctgaa aattcatgcc
     1321 cgtgaagtca gcttcgctgg gcaaaaagac aaacatgctg ttacggaaca gtggttatgc
     1381 gctcgcgtgc cgggcaagga aatgcccgat ctgagcgcct ttcaactgga aggctgccag
     1441 gtgctggagt atgcgcggca caagcgcaag ctgcgtttag gcgcgctgaa aggtaacgcc
     1501 tttaccctgg ttctgcgcga agtgagcaat cgcgatgacg ttgaacaacg tctgatcgat
     1561 atttgcgtaa aaggtgtacc gaactacttc ggtgcccaac gttttgggat tggcggtagc
     1621 aacttgcagg gggcgcagcg ctgggcgcaa accaatactc cggtgcgcga tcgcaataaa
     1681 cggagttttt ggttgtcggc agcccgcagt gcgttgttta atcagattgt tgctgagcgc
     1741 ctcaaaaaag cagacgttaa tcaagttgtt gacggcgatg cgctacaatt agccggacgt
     1801 ggtagctggt ttgtcgcaac caccgaagaa ctggcggaat tacagcgtcg cgttaacgat
     1861 aaagagctga tgataaccgc cgcattgccg ggcagtggcg aatggggaac tcagcgtgaa
     1921 gcgctggcat tcgaacaagc agctgttgcc gcagaaactg aattacaagc tttactggtg
     1981 cgcgaaaaag ttgaagccgc gcgcagagcg atgctgctgt atccgcaaca attaagctgg
     2041 aattggtg
//
LOCUS       DAEVZA010000298         1951 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEVZA010000298 DAEVZA010000000
VERSION     DAEVZA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.300"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..1011
                     /locus_tag="LAF92_005171"
     CDS             <1..1011
                     /locus_tag="LAF92_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI9794041.1"
                     /translation="GEQAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEIL
                     ADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATE
                     WCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPI
                     GLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQL
                     RLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTA
                     SDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGI
                     RK"
     gene            1008..1577
                     /locus_tag="LAF92_005172"
     CDS             1008..1577
                     /locus_tag="LAF92_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794042.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
     gene            1803..>1951
                     /locus_tag="LAF92_005173"
     CDS             1803..>1951
                     /locus_tag="LAF92_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBI9794043.1"
                     /translation="MELKKLMEHISITPDYRQAWKVVHKLSDILLLTICAVISGAEGW
                     EDIED"
BASE COUNT          607 a          388 c          465 g          491 t
ORIGIN      
        1 ggtgagcagg cgaaaacgca gcgccgcagc ctggcggata cccttcagca gtccggcggc
       61 gaagacggtg gcagtgtggt gttcccgccg gtgctggtgc agatgctcga ccggctggaa
      121 agtgaaatcc tggctgaccg ggtgagtgag gaaagccgcc gctggctggc atcgtgcggc
      181 ctgacggtgg agcagatgca aaaccagatg gacccggtgt acacgccggc gcgaaaaatc
      241 cacctgtacc actgcgacca tcgcggcctg ccgctggcgc ttgtcagcac ggaaggggca
      301 acagaatggt gcgcagaata cgatgaatgg ggcaacctgc tgaatgaaga gaacccgcat
      361 cagctgcagc agcttatccg cctgccgggg cagcagtatg atgaggagtc cggcctgtat
      421 tacaaccgcc accgctatta tgacccgctg caggggaggt atatcactca ggatccgatt
      481 gggctgaagg ggggatggaa tttttatcag tatccgctga atccggttca gtatatagat
      541 tcaatgggac tggcatcaaa atatggacac ttaaataatg gcggatatgg agcgagaccc
      601 aacaaaccgc ctacgcccga tccaagtaaa ttgccggaca tagcgaaaca attaagactg
      661 ccatatccta ttgaccaggc cagtagtgcg cctaatgttt tcaaaacatt cttcagagca
      721 ttaagccctt acgactacac actgtattgc aggaagtggg taaaaccaaa tctgacttgt
      781 acgccacagg atgattccca gtatccaggg atggatacaa agacagcaag tgattacctg
      841 ccacagacaa attggccaac aactcaatta ccaccaggat atacttgtgc agaaccctat
      901 ttattcccag acattaataa acccgatggg ccagcaacag cagggataga tgatttgggt
      961 gaaattttag ctaagatgaa acagagaaca tcgagaggaa taagaaaatg aaaagagttt
     1021 tgttcttttt gctgatgata tttgttagtt ttggtgttat agctgattgc gaaatacaag
     1081 ctaaagatca tgattgtttt actattttcg ctaagggtac tattttttcc gcgtttcctg
     1141 tcttaaataa taaagccatg tggagatggt atcagaatga ggacatcggt gagtattatt
     1201 ggcaaacaga actgggtaca tgtaaaaata ataaattcac accaagcggg gcgagactct
     1261 taattcgcgt cgggtcacta cgccttaatg aaaatcatgc catcaaagga acgttgcagg
     1321 agttgataaa tacagcagaa aaaacggcgt ttctcggcga tcggtttaga agttatataa
     1381 gagcgggtat ctatcaaaaa aagagcagtg atccagttca gttgttggca gtccttgata
     1441 attcaatcat ggtaaaatat tttaaagatg agaaaccaac ttatgctcga atgacagcac
     1501 atctcccgaa caaaaatgag tcttacgaat gtttaataaa gatacagcat gaactaattc
     1561 gtagtgaaga gaaataaaat ctcttcctga gaaaacaaat taatcattgt gaaaaattat
     1621 ataactcatg gaataggtgt ttattttgcg ttccatttgc agggaaagat cacgtaacgc
     1681 tacttttttg tactaaataa ttcgaatttt aagttcaaca attgagatac tacttattgt
     1741 ctaaagctgt ttttcatagc ttatacatga tcaaatactc cttacataaa taaggtgaac
     1801 aaatggaact taaaaaattg atggaacata tttctattac acccgattac agacaagcct
     1861 ggaaagtggt gcataaattg tcagatattc tactgttgac tatttgtgcc gttatttctg
     1921 gtgcagaagg ttgggaagat atagaggatt t
//
LOCUS       DAEVZA010000299         1930 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEVZA010000299 DAEVZA010000000
VERSION     DAEVZA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.301"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..143)
                     /locus_tag="LAF92_005174"
     CDS             complement(<1..143)
                     /locus_tag="LAF92_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794044.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVL"
     gene            complement(236..670)
                     /locus_tag="LAF92_005175"
     CDS             complement(236..670)
                     /locus_tag="LAF92_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HBI9794045.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            960..1259
                     /locus_tag="LAF92_005176"
     CDS             960..1259
                     /locus_tag="LAF92_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794046.1"
                     /translation="MAAEGLTAPSGAVALHTVRCAGSAVSGATGAARPERSLQAWFFR
                     HRTVAAARNRASGFGGDWRVRQQLPVGVGPDDRMPPGSREPKVSPPPEIRTTETT"
     gene            complement(1399..1899)
                     /locus_tag="LAF92_005177"
     CDS             complement(1399..1899)
                     /locus_tag="LAF92_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBI9794047.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT          419 a          584 c          562 g          365 t
ORIGIN      
        1 aggacgtccg ggttaatctc atcctgtgcc agctcctgac gtctgaagcg ggtatggcgg
       61 tgcaggggtt tattctcccg caggcactct tcacgcagac gctttacata ctcggcggtt
      121 gcgaagataa ttgatggtct catggcataa tctccataga tagtgattga gatgccggac
      181 ttcctggcgg ggaagcgccg gcggagagag gcagggttta tcgccctgcc ttttttcatt
      241 tcggacagcg acgcagtgcc agcgcgagcg ctttcatctt ccagccttcc ggcgtgcggt
      301 acatcacctt ccagtagccg tcgatgaaca cataatggaa gcgcacctct tcagtgcttt
      361 cagtggcaat agcctccctg aaagaacggt actcacgcgc ctcactgtcc atccggaaac
      421 cactgtcacg tccgtaataa gccgtcacac cgtccaccgg gttctgaagc gtatgcccgg
      481 cgggtttgtc gcaacgggga ccgaggcaac ggatatcacc gtgttcaacc agggcttcgg
      541 cctgctgacc ggtggcatag tgggtatgca ggatgcgacc gttccaggtc ggctcaccgt
      601 gcaggtggca gtaaacggta cggaacaccc cgtcgcggca aaaaacggaa atcgtgctgc
      661 tgacagtcat gaaaacgtct ccttctgctg aatcaggtcg ccgggccgac cggttgctcc
      721 ccggaggggc gaaagcccct tctgccgtcc acttcttcgg ggtttctccc cgcagtggac
      781 ctttccgatg gggcgtccgt caccacgtca cggggcagct gtccgggggc ggacgcaaaa
      841 ttttttgccg ggtttatggc taaaaatttt gtggacggta tatcttcccc tggactgctg
      901 aaacgggatg tggttactcc agcccccgga aagtccacag cgggaaacag gccgcagggg
      961 tggcggcgga agggttgact gcgccatcag gcgcagtggc cttacacacc gtcaggtgtg
     1021 cagggagcgc cgtttccggc gcgacagggg cggcgcgacc ggaacggtcg ctgcaagcct
     1081 ggtttttcag acatcggacc gtagcggcag cccgaaaccg cgccagcggg ttcggcggag
     1141 actggcgggt gcgccagcag ctgcctgtcg gagtcgggcc tgacgaccgg atgccgccag
     1201 gcagccggga gccgaaggtg tccccgcctc cggaaatcag aacaacagaa acgacataac
     1261 agaaaaatgc ctcacaccat gacgggcagg aagggcagcg ccgcaggcgc ggccctcctg
     1321 catttcaggc aggcgtaagc ctgccagggt tttccggcgg cctggcaggc catacccccg
     1381 ccggagcggg ggagtaaatc accgacggaa cacatgaccg tcaataaagg tgaaggagtc
     1441 caggaataaa tcccgcgcat acgcctcata gtcaaaatag agcgccaccg tctccggcac
     1501 gtcagccagc aggccggtat cactgacgaa ctcaaccgca aaagcctcct cactgtcagc
     1561 ctcgccccac caggcatcgc ggaaggtgtc aaaatccgtc tcaccggtat catccaccca
     1621 gagacgataa gcctcttcgc agccttcatc ccgtgcccgg cggaagcctt caacccaagc
     1681 ccagttgata tggcattcag aggccatatt ccccgggaat ccctcataat cctgaaacat
     1741 cagttcagga tcggcttcat cctggtgaag agcacggcag gcggcgaaaa agtcgcgctc
     1801 atcatcaaac gtggtcaggt caaaccagcg tccggcgatg cttccacagt tgtatttgtg
     1861 ccaggttcca acgtatacag caggtgcaac aacagacatt cccgaatcct ccatagtcac
     1921 atgttctgcg
//
LOCUS       DAEVZA010000300         1915 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEVZA010000300 DAEVZA010000000
VERSION     DAEVZA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.302"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            548..1906
                     /locus_tag="LAF92_005178"
     CDS             548..1906
                     /locus_tag="LAF92_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI9794048.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
BASE COUNT          574 a          304 c          407 g          630 t
ORIGIN      
        1 tctaattaca ggtaactgaa aagaaagata tttttgcacc tcataatccg ttattaaacg
       61 cggaagagag acgtgaattg ttgatgatga gaagaagaaa tgatgagcag agtgtccata
      121 taaaatcctt ttctcgcccg aaaatccatt ccaatgatga ggatcttcag gaatacggca
      181 taaatcccaa tgcctttttc aaaataaatt aggagtaaaa taattaaatc agtaaattcc
      241 gatgcatgat ttcggatttt tcgaaagtcc tgatgaaagg ctgcgctacg gcactcacgg
      301 atttaattgt tattcctaat gtatctatga attaatgttt tataaggatt ttctataacc
      361 atttataagt attttcaagc ctggcttgtt gcaaacaatg tataaagcac ttaggcaata
      421 ataattacat tcagcaacta tcatcatcgg tattgtttgt gggcggaatt tcatattgac
      481 aacagtacag ttcttatatc tattaataat agaaagggat ctacaaccta cagattggtg
      541 tagctttatg gaaaaagact atttgagaat tagtagtact gtattagtga gcttattgtt
      601 tgggcttgct ttggttttgg tgaatagttg gtttaatcag ccaggcgttg aggaagttgt
      661 tccacgatca acctatctga tggtaatgat tgctttattt tttatcgata ctgttgcatt
      721 tatttttatg cagttgtatt tcatttatga ccgtaggcaa ttttcaaact gtatacttag
      781 tttggctttt ctgagttgtt tgatttactt tgttaaaacc gtcattatca ttcagcaaat
      841 tattgagggg cgtttgacaa gcagtgttgt ccaaaatgac attgcaatct attatttgtt
      901 tcgtcagatg agtttgtgca tattaatatt tctggcattg gtgaataaag ttagtgaaaa
      961 cacaaaacag cgcaatttat tttccaaaaa aatgactttg tgcataagtt tgttttttgt
     1021 ttttgggggg ccgatagttg ctcacatact ttctagtcat tatgagagct acaatttaca
     1081 cattgccgag ttaaccaatg aaaatggtca ggtggtttgg aaagcctcat atgttaccat
     1141 aatgattttc atgtggttaa ccttgctttc ggttaatcta tactttaatg gtttacgcta
     1201 tgatatctgg aatggagtga cggtgattgc tttttgtgcc gtactgtaca atatctctct
     1261 gttatttatg agtaggtata gcgtgtccac ttggtatatt agtcgcacta ttgaagtagt
     1321 cagtaagtta actgttatgg taatatttat gtgccatata ttcagtgcgc tacgagtaac
     1381 aaagaacatt gcacatcgcg atcccttaac caatatattt aacagaaatt atttttttaa
     1441 tgaactgaca gttcaatcag catcagccaa aaaaacgcct tattgcgtca tgattatgga
     1501 tatcgaccac ttcaaaaaag tcaacgacac ctgggggcat ccggttggcg atcaggtgat
     1561 aaaaacagtg gtgaatatca ttgggaaaag catacgacca gatgaccttt tagcgcgcgt
     1621 cggcggcgaa gagtttggcg tcttgctgac ggacatcgat actgaacgtg cgaaagcttt
     1681 agcggaaagg attcgggaaa atgttgagcg tttaactggc gataatcctg aatacgctat
     1741 accacaaaaa gtgacgatta gtattggcgc tgttgtgact caggaaaatg cgttaaaccc
     1801 aaatgagatc tatcgactgg ctgataacgc actttacgag gcgaaagaga ccgggcgtaa
     1861 taaggtggtt gtgagggatg tggtgaattt ttgtgagtca ccataaagcg gcatt
//
LOCUS       DAEVZA010000301         1909 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEVZA010000301 DAEVZA010000000
VERSION     DAEVZA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.303"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..848)
                     /gene="sohB"
                     /locus_tag="LAF92_005179"
     CDS             complement(<1..848)
                     /gene="sohB"
                     /locus_tag="LAF92_005179"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBI9794049.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYG"
     gene            1068..1826
                     /locus_tag="LAF92_005180"
     CDS             1068..1826
                     /locus_tag="LAF92_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBI9794050.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            1823..>1909
                     /locus_tag="LAF92_005181"
     CDS             1823..>1909
                     /locus_tag="LAF92_005181"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="catalyzes the formation of adenosylcobalamide from
                     cobinamide and the formation of coenzyme B12 from
                     cob(I)alamin; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBI9794051.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERG"
BASE COUNT          469 a          524 c          450 g          466 t
ORIGIN      
        1 tgtccgtacc agtgttcacc cgttgccacc tgttcaatat ccagagacgg acgcatacgc
       61 ttcacaaaat ctttaaacaa ctgatgcgtt tcgttcaact cttcgcggaa tttctcccgc
      121 ccttcttcgg tattttcacc cagcaacgtc agagtacgct tatactgccc ggcagtgtgc
      181 agttcgatat caatatcttt gcttttcagg aagcggttaa agttgggcat ttgcgccacc
      241 accccaatgg aacccacaat agcaaacggt gcggaaacaa ttttgtccgc cacacaggcc
      301 atcatgtaac cgccgctggc agcgactttg tctaccgtaa cagttaaagg aatgttttta
      361 tcacgcagac gctgcagctg cgaagccgcc aacccgtaac catgcaccat gccaccaggg
      421 ctttccagac gtagcacaac ctgatcctgc ggtttgaatg ctgcgagtac agccgttatc
      481 tcttcacgta gcgagttcac ttcatgggcg tccatgctgc ctttaaaatc cagcacccag
      541 acgcgaggtt tactgtcagt tgccacctcg cccagtttgg ctttcgcttt tgctgctttc
      601 gcttcttgct tgtgcttttt cttctgcgct ttgtgccact gtttttgctg atgtgagtcc
      661 atcagcgccg cggccagttc ttctttcatc tccttatact gttcgctgag attgttgacc
      721 cgtaactcgc cacgctggcg tttattacgt tgagcaacat tgacaataat ggcggcaatc
      781 gccgcaatcg ctagcacaac ggtaacgatt ttcgccaaaa acaaaccata ttcagacaac
      841 aattccacga gtccccacct tggttaaaca acgctttggc tcacagttta caacaggcat
      901 gttaagccgt ctcgcagaga tgttattgca gcgaaagtat gacaaagtat ccattttgcg
      961 gtgttatcgc cccaggctga ttgaaatgcc cttctgtttc aggcataaag ccccaaagtc
     1021 ataaagtaca ctggcagcgc gacagacaac gtcgttgagg agtagccatg cattaccagc
     1081 caaaacaaga tttactcaat gatcgcatta tcctggtgac gggagccagc gatgggattg
     1141 gccgtgaagc cgcgatgacg tatgcacgct atggtgcgac agtgattctg ttgggccgta
     1201 atgaagaaaa attacgtcag gtagccagcc acataaacga agaaactgga cgtcagccac
     1261 agtggtttat tctcgatttg ctgacctgca cgtccgaaga ttgccaacaa ctggcacagc
     1321 gcattgtcgt taattatccg cgtctggatg gtgttttgca taatgccgga ttgctcggcg
     1381 atatttgccc aatgagcgaa caaaatccgc aggtctggca ggacgtcatg caggtcaacg
     1441 ttaatgccac ctttatgctc acccaggcac tgcttccttt attactcaaa tcggacgccg
     1501 gttcactggt ctttacttca tcaagcgttg gacgtcaggg acgagctaac tggggtgcat
     1561 atgcagcgtc gaaatttgcc accgaaggga tgatgcaggt actggccgat gaatatcagc
     1621 agcgcctgcg cgtcaactgc attaacccag gcggtacgcg caccgcaatg cgcgcaagcg
     1681 ccttcccgac cgaagatcca cagaaactta aaacacccgc tgatatcatg ccgctctacc
     1741 tctggctgat gggcgatgac agccgccgta aaaccggcat gacctttgac gcccaaccgg
     1801 gccgtaaacc aggaatttcc caatgagtga tgaacgctac caacagcgtc agcagcgagt
     1861 gaaagaaaaa gtagatgctc gtgtggccca ggcccaggat gaacgcggt
//
LOCUS       DAEVZA010000302         1906 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEVZA010000302 DAEVZA010000000
VERSION     DAEVZA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.304"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..409)
                     /locus_tag="LAF92_005182"
     CDS             complement(<1..409)
                     /locus_tag="LAF92_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBI9794052.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTEDRVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICNTAAIARIFVQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYIILEAARNYWSALDDEKKSRFRFHHISTDEV"
     gene            complement(779..1672)
                     /gene="galF"
                     /locus_tag="LAF92_005183"
     CDS             complement(779..1672)
                     /gene="galF"
                     /locus_tag="LAF92_005183"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBI9794053.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT          512 a          463 c          395 g          536 t
ORIGIN      
        1 acacttcgtc agtagaaata tggtgaaagc ggaagcggct tttcttttca tcatccaatg
       61 cagaccagta attacgagcg gcttctaaaa taatataggt acccacaata ttggtttcaa
      121 taaatgcagc cgggccagtt attgaacgat caacgtgact ctcagccgcg aggtgcatca
      181 ctgcatctgg ctgatgctga acaaaaatac gcgctattgc tgcagtattg caaatatccg
      241 catgttcaaa aatataacgt tcagaatcag aaacatcagc gagtgattcc aggtttccgg
      301 cgtacgttaa tttatcgaca ttaacaacac gatcctccgt attatttata atgtgacgaa
      361 ccacagcaga accgataaat cctgcaccac cagtaacaag tatcttcact ttttattcca
      421 aattacgttc agagctcact gtggaataaa cggctcttcg tttgattaac agaagtatta
      481 acgcacgcta ccgcccctgg cttaacagct accagagcac tgcgtgcata ccgacgatgt
      541 ggcgagtgtt acccactcgc gctaaacaaa aaaaattcaa acgctaattg tcttaccaat
      601 ctgctcagga aacaaggaaa atccaggaaa actttgacta aaatcttatt gctaactcat
      661 tgttattata gtgttttata aagaaacaac gacaagaaca tttgttgcaa tttactttcg
      721 ctacgaatct tcactgccgt tatgattttc ttattgaccg ttacatccgg taagattttt
      781 attcgcttaa cagcttctca atccctttgc ggaacttcgc tccttctttc aggttgcgta
      841 gtccgtactt cacaaatgcc tgcatgtaac ccattttttt accgcagtcg tagctgtcac
      901 ccgtcattaa cattgcgtca acggactgtt ttttcgccag ttcggcaatg gcgtcagtta
      961 gctgaatacg tccccatgct ccgggttgag tccgctccag ttcaggccaa atgtccgcag
     1021 aaagcacata gcgaccaacc gccataatgt ctgagtccag cgtctgcggc tgatcgggtt
     1081 tttcgataaa ttcaacaatg cgactaactt tgccttcacg atccagcggt tctttagtct
     1141 ggatgacaga gtattcagaa aggtcacccg gcatacgttt tgccagaacc tggctacgac
     1201 ccgtttcgtt gaatcgcgca atcatggcgg caaggttgta acgcagcgga tcggcgctgg
     1261 cgtcatcaat aacgacatcc ggcagcacca caacgaatgg attgtcgcca atggcaggtc
     1321 tggcacacag aatggagtgg cccaaaccta aaggttcgcc ctgacgcacg ttcataatgg
     1381 taacgcccgg tgggcagatg gactgaacct ctgccagcaa ctggcgtttt acgcgcagtt
     1441 caaggagaga ttctagttca taagaagtgt cgaagtggtt ttcgactgcg ttcttggacg
     1501 catgagtcac caggaggatt tctttgatcc ctgcagccac aatttcgtca acaatgtact
     1561 gaatcatggg tttatcgacg atcggtagca tctctttggg tatcgcctta gtggcaggca
     1621 acatatgcat cccaagtccc gctacaggaa taactgcttt taaattcgtc attatttcat
     1681 ccacctgtaa aatggttgct gaattatagc tcgttcgatt tttttcgcca gcatcaatta
     1741 tcctgaactg aatactgatt tactgccgat gctacgccgc ttcgttcagg attgcagtag
     1801 cgatattttt aagtatggtt ccatttttcc aggaatggtc gcaaatctcc tcccctcttc
     1861 tggctgtcga aagttaatct tttccacatt aataatacgg tgatta
//
LOCUS       DAEVZA010000303         1905 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEVZA010000303 DAEVZA010000000
VERSION     DAEVZA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.305"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(306..1610)
                     /gene="ndh"
                     /locus_tag="LAF92_005184"
     CDS             complement(306..1610)
                     /gene="ndh"
                     /locus_tag="LAF92_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBI9794054.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          466 a          486 c          472 g          481 t
ORIGIN      
        1 cgggccccgt tcaaacacgt tcagactggc cactgccgct acgcccagcg cagctgcgac
       61 accaatcccg atacccgcca acattgattt attcacggga acacctcctt ctttgcagtt
      121 atgccaatca tgcaagcgaa ggagaaaagc agctatcaga cagaagatga aaagcgcggg
      181 aatggctgga aatacttaag gattcggagg agtctgaagg aatgcctgat gcgacgcggc
      241 tgccgatcgc gctgttcctc aagggcggat aaggcgttta tgccacatcc gccagtgtac
      301 gtcgattaat gcaacttcaa acgcggacgg ataacgcggt taatactccc caccagcatc
      361 attaatccgg ttttaaagta accatgcagc gcaatctgat gcattcggta tagcgagata
      421 tatacaaagc gcgcaattcg tccttcaatc atcattgagc cgcgcgtcag gttacccatc
      481 aggctaccga cggtggagaa gttcgacagc gataccagcg aaccatgatc tttatactga
      541 taatttttca gcggcttacc gttcatctgc gccagtatgt tgttcattgc gcaagtcgcc
      601 atctggtgtg cagcctgagc acgcggcgga acaaagcccc cttccggacg cgggcatgac
      661 gcgcagtcgc caatagcgta aatgtctgga tcgcgggtgg tttgcagcgt cggttccacc
      721 accagctggt tgatacggtt agtttcaaga ccaccgatat ctttcaggaa gtctggcgct
      781 ttgatcccgg ctgcccacac catcagatca gcctcaatat attcgccatc tttagtgtgc
      841 aggccgcctt catcagcact ggtgaccatg gtttgcgtca gcacgcgaac gccaagtttc
      901 gttagctcgt tgtgggccgc agcagagata cgtggcggta atgcaggcaa aatacgttct
      961 cccgcttcta ccagcgttac gttcagggct tcgttggtca ggcctttgta accgtagctg
     1021 tgcagttgct tgaccgcgtt gtgcaattca gcggagagtt ctacacccgt cgcgccgccg
     1081 ccgacaatcg caatgttcac tttgccattt gcgcccaggt tggcggagta tttcaggaag
     1141 agattcagca tctcctggtg aaagcgacgc gcctggtgcg ggttatcgag gaaaatgcag
     1201 ttctctttga cacctggcgt attgaaatcg ttagaggtgc tacccagcgc cattaccagg
     1261 gtgtcatagg cgattttacg ttccggaacc agcagttcac ctttctcatc gcgcagttct
     1321 gcaatagtga ttgttttcgc ttcacgatca atatcaatga cggaacccag ctggaactgg
     1381 aaaccatgat tgcgcgcatg ggccagatag ctcaacgcat cgacgccttc atcaagcgag
     1441 ccagtcgcca cttcgtgcag cagcggtttc cacaggtggc tgtggttacg atcgaccagc
     1501 gtaattttgg cttttttctt acgtcccagc ttatgcccca gctgtgttgc catttccagc
     1561 ccaccagcac cgccgccgac aatcacaatc tttttcaatg gcgtagtcaa cgtgaccccc
     1621 ttaaatttat taactaattg ttaattaatg gttattaaca tagctcttaa ttaacaacag
     1681 gttacagcaa tgaaaatgtt cttcgctatt gagaataaca tgaatggtgc attggtcata
     1741 ccaaaattga tgttaatcaa gttttgttgc tgaaaagata ggcgacttta ccgggtgaga
     1801 tcaaaaaata gagtttattg tttattattt gagcaaattc ggaagagtaa tatagggtga
     1861 agcgtatatc gagggcgttt ggtcgtcctg ataagatgcg cagca
//
LOCUS       DAEVZA010000304         1891 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEVZA010000304 DAEVZA010000000
VERSION     DAEVZA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.306"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(17..754)
                     /locus_tag="LAF92_005185"
     CDS             complement(17..754)
                     /locus_tag="LAF92_005185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794055.1"
                     /translation="MALPFAADACLLASSFEADADDADDAAELADDAAAIFEDSALVS
                     DAFAFVSDVFAADADLAAALACSVASPAFVVAVEADDAALSADFPAAVALAEACPALV
                     DAALADDAAAVFEPAAAEALCPAAVSEDLAFVSDVFAAFAEFPAAVAEEAALLALEDA
                     FVSDDFAASFEAAASRAEVAASDAFVVAADAEVAADCCDAAAFVSDVFAAAALVAAAL
                     FEDSAAAALFDASVAFVDAVPALEDAD"
     gene            891..>1891
                     /locus_tag="LAF92_005186"
     CDS             891..>1891
                     /locus_tag="LAF92_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBI9794056.1"
                     /translation="MEDASLTTKGVVKLSSAVDSTSESLAATPKAVKAANDNANSRVP
                     SNRKVNGKALTADITLTPKDIGTLNSVTMSFSGGAGWFKLATVTMPQASSIVYIALIG
                     GAGYNVGSPHQAGISELVLRAGNGNPKGITGALWKRTAVGLTNFAWINTSGDTYDIYV
                     EIGNYATSVNIHWDCTANASVSVYTSPTYSASKPSSVTYGVVYTMYSSHQKPTPSDIG
                     ALPTTGGTVSGPLSVTGGLTGSLNGNASTATKLQTARSIGGVVFDGSANINLPGVNTT
                     GNQNTTGNAATATKLQTARKISGVPFDGSKDITLTAAHVAAFARRATDTYADADGGVP
                     "
BASE COUNT          517 a          496 c          510 g          368 t
ORIGIN      
        1 aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg aacggcatca acaaaggcca
       61 ctgaagcatc gaaaagtgct gctgctgcag agtcctcaaa aagcgcggca gctaccagtg
      121 ccgctgcggc gaaaacgtca gaaacgaatg cggcagcgtc acaacaatca gcagccactt
      181 ctgcatccgc cgcgaccacg aaggcgtcag aagctgccac ctcagcccgg gatgcggcgg
      241 cctcaaaaga ggcagcgaaa tcatcagaaa cgaacgcatc ctcgagcgcc agtagcgcag
      301 cttcctcggc aacagcggca ggaaattccg cgaaggcggc aaaaacgtca gagacgaacg
      361 ccaggtcttc tgaaacggca gcgggacaga gcgcctcggc tgcggcaggc tcaaaaacag
      421 cggctgcgtc gtctgccagt gccgcgtcaa caagtgccgg gcaggcctca gccagtgcca
      481 ccgccgccgg aaaatcggca gaaagcgctg catcgtccgc ttcaacagct acaacgaagg
      541 ctggcgaagc cactgaacag gccagcgcag cagcgaggtc tgcatccgca gcgaagacat
      601 ccgaaacgaa cgcgaaagcg tcggaaacca gcgcagaatc ctcaaaaatg gcagctgcat
      661 cgtccgccag ttcggcggcg tcatcggcat catctgcgtc tgcttcaaaa gatgaggcga
      721 gcagacaggc atcagcagcg aaaggcagcg ccacgacagc atccacgaag gcgacagagg
      781 cagctggcag tgcgacggcg gcatctcaga gcaaaactac tgctgaatcc gctgcgaccc
      841 gtgcggaagc tgctgctgat cgtgctgaag agattgccgg tgcagttgcg atggaagacg
      901 caagccttac aactaaaggt gttgtgaaac ttagcagtgc tgttgatagc accagtgaat
      961 cgctggccgc aacgccaaaa gcagttaaag cagccaatga caatgcgaat agcagggtgc
     1021 catctaaccg aaaagttaac ggaaaagcac tgactgcgga tataacatta acgccgaaag
     1081 atattggtac tttaaattca gtaacgatgt ctttctctgg cggggctggg tggttcaaac
     1141 tggctacggt taccatgcca caagcgagtt ccatcgttta catcgcattg attggtggcg
     1201 ctggttacaa cgtcggttcc ccacatcagg caggcatttc agaactggtt ctacgagcag
     1261 gcaatggaaa ccccaaagga attaccgggg ctttgtggaa gcgtacagcc gtcggattaa
     1321 cgaatttcgc ctggatcaac acatccggcg atacatatga tatttacgtt gagattggca
     1381 attatgcgac gagtgtaaat atccattggg attgtactgc aaatgcgtca gtttctgttt
     1441 atacctcgcc aacatattca gcgagtaagc cttccagcgt tacctatggt gttgtttata
     1501 cgatgtatag ctcacatcag aaacctacac catcagatat tggagcactg ccaacaaccg
     1561 gaggaacagt ttcaggtccg ttgtctgtta caggtggatt aactggttca ttgaatggta
     1621 atgcaagtac agccacgaaa ttgcagacgg caagatctat cggtggagtt gttttcgacg
     1681 gttctgcaaa tattaacctt ccaggtgtaa acactacggg taatcagaac accactggta
     1741 atgctgcaac tgctacaaaa ctccagactg caagaaaaat atccggtgtt ccatttgatg
     1801 gttccaaaga tattacttta accgccgcgc atgtggctgc ttttgccaga agggcaacgg
     1861 atacatatgc cgatgcggat ggtggcgttc c
//
LOCUS       DAEVZA010000305         1876 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEVZA010000305 DAEVZA010000000
VERSION     DAEVZA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.307"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..337
                     /gene="yddG"
                     /locus_tag="LAF92_005187"
     CDS             <1..337
                     /gene="yddG"
                     /locus_tag="LAF92_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBI9794057.1"
                     /translation="FNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIKLISAAFTL
                     GFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGALMVCGGSL
                     LCWLATRRG"
     gene            596..886
                     /locus_tag="LAF92_005188"
     CDS             596..886
                     /locus_tag="LAF92_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI9794058.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            910..>1876
                     /locus_tag="LAF92_005189"
     CDS             910..>1876
                     /locus_tag="LAF92_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBI9794059.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTAINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFL"
BASE COUNT          621 a          378 c          304 g          573 t
ORIGIN      
        1 atttaacgga attaccgttt ttgtcctgct aacgggagca agtctgtggg tttactattt
       61 tctcacgcca caaccagaaa tggtatttag cacgcccgtc atgattaaac tcatctctgc
      121 ggcatttacc ttaggatttg cttatgctgc atggaatgtc ggtatattgc atggtaacgt
      181 caccataatg gcggtaggtt cgtattttac gcccgtactt tcctcagcgc ttgcagccgt
      241 gctgctcagc gccccgctgt cgttctcatt ctggcaaggc gcgctaatgg tctgcggcgg
      301 ttccctgctc tgctggctgg cgacacgtcg tggttaaaat aattgccggg tctacggccc
      361 ggcagttaat cagataaatt ccgacaatat taaaaaataa atttcaaata ccagtaattc
      421 accttatatc tcatttcgac ttcgatcaca tatcgataaa taatattatt tcatctattt
      481 ttctcattgt ttttaatcac cattctattt taaaaacagt atatcattta taaatgacgc
      541 cgtttaaaat tcgtttagaa aatacgttta acgataatta aaaggattct gttaaatgaa
      601 attaaaaata gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag cacacgctgc
      661 cgaagtctat aacaaggatg gtaataaact cgacctttat ggcaaggtta ccgctctacg
      721 ttattttact gatgataagc gtgacgatgg tgataaaact tatgcccgtc tcggctttaa
      781 aggagaaacg caaatcaatg atcaaatgat tggttttggt cactgggaat atgattttaa
      841 aggctataac gatgaagcca acggctcgcg cggcaagaat ctctgattga gttaagtgcc
      901 catcacaata tgatcactga ccttatatta cacaatcatc ccaggatgaa aacaatcact
      961 ttaaacgaca accatattgc acatttaaac gccaaaaaca ctacaaaact ggaatattta
     1021 aacttaagca ataacaattt actgccaacc aatgacattg atcaactaat atcatcaaaa
     1081 catctttggc atgtattagt taacgtcatc aacaatgatc cacttgcaca aatgcagtac
     1141 tggactgcag taagaaatat aattgatgac actaatgaag tgaccattga tttatcagga
     1201 cttaatttaa ccactcaacc accagggctg caaaacttca ccgctatcaa tcttgataat
     1261 aaccaactca cacattttga tgcaaccaac tacgatagac tcgtaaagct aagtctgaat
     1321 agtaatgctc ttgagtcaat aaattttcct caaggcagaa atgtaagtat tacacatata
     1381 tctatgaata ataatgctct cagaaatatt gatatagata ggctttcatc agttacttat
     1441 tttagtgcgg cacataatca actagagttt gtgcaattag aatcttgcga atggctgcaa
     1501 tacctgaatc tcagccataa tcaattaact gatattgttg caggtaataa aaatgaactc
     1561 ttactgctgg atctatccca taataaacta acaagtttac acaatgactt atttcccaac
     1621 ttgaatacgt tacttattaa caacaatttg ctttctgaaa ttaaaatatt ctatagcaac
     1681 ttctgcaatg ttcagacatt aaacgctgct aacaaccagt tgaaatatat aaatcttgat
     1741 ttcctgactt atcttccatc tatcaaaagt ttaagactgg acaataataa aataacccac
     1801 attgatacta ataatacatc cgatattgga actttattcc ccataataaa acagagcaaa
     1861 aacttaaatt ttttaa
//
LOCUS       DAEVZA010000306         1851 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEVZA010000306 DAEVZA010000000
VERSION     DAEVZA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.308"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..200
                     /gene="thiK"
                     /locus_tag="LAF92_005190"
     CDS             <1..200
                     /gene="thiK"
                     /locus_tag="LAF92_005190"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="catalyzes the phosphorylation of thiamine to
                     thiamine phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBI9794060.1"
                     /translation="HRQLVNDYATRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQ
                     TGDQQFIRLADDTWRQLLIKQ"
     gene            211..1236
                     /gene="nagZ"
                     /locus_tag="LAF92_005191"
     CDS             211..1236
                     /gene="nagZ"
                     /locus_tag="LAF92_005191"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBI9794061.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            1259..1801
                     /locus_tag="LAF92_005192"
     CDS             1259..1801
                     /locus_tag="LAF92_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI9794062.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
BASE COUNT          464 a          431 c          517 g          439 t
ORIGIN      
        1 agcaccggca attggtcaat gactatgcca ctcgcgcgaa gatttatccg gcgcaattat
       61 ggcgtcaggt caggcgatgg tttccctggc tgctgatgct caaagcaggg tggtttgagt
      121 accgctggcg acaaaccggc gatcaacaat ttatcaggct ggccgatgac acctggcggc
      181 agctattaat aaaacaataa ggagagcagt gtgggtccag taatgttgga tgtcaaaggt
      241 tacgaactgg acgcggaaga gcgtgaaata cttgcgcatc cgctggtggg agggctgatt
      301 ctctttacgc gtaactatca tgatcctgcc cagttacgtg aactggtgcg ccagatccgc
      361 gcagcttcgc gcaatcatct ggtggtggcg gtagatcagg aaggtggacg cgtgcagcgt
      421 tttcgtgaag gttttacccg cttgccagcg gcgcaatcat tcgctgcgct gtcaggaatg
      481 gaagagggcg gcaaactggc gcaagaggcg ggttggctga tggccagcga aatgatcgct
      541 atggatattg atatcagctt tgcgccagtg ctggatgtcg ggcatatcag cgcggcgatt
      601 ggcgagcgtt cttatcatgc cgatccacaa aaagccctgg caattgccag ccggtttatt
      661 gatggtatgc atgaagccgg aatgaaaacg accgggaaac acttcccagg acacggtgca
      721 gtaacggcag actcacacaa agaaacaccg tgcgatccac gtccacaagc ggagattcgc
      781 gctaaagata tgtcggtctt cagttcctta atccgcgaaa ataaactcga cgccattatg
      841 cctgcgcatg tgatctacag tgatgttgat ccgcgtccgg cgagcggttc tccctactgg
      901 ctgaaaaccg ttttgcgtca ggaactgggt tttgacggcg tgattttctc tgacgattta
      961 tcgatggaag gtgccgcgat tatgggcagt tatgccgaac gcgggcaggc atcactggat
     1021 gcgggttgcg atatgatcct ggtctgcaat aatcgtaaag gggccgtcag cgtgttagat
     1081 aatctgtcac cgatcaaggc agaacgtgtt acacgtttgt atcataaagg ttcattttcg
     1141 cgacaggaac tgatggactc ggctcgctgg aaagcgatca gcacccgtct gaatcagtta
     1201 cacgaacgct ggcaggaaga gaaagcaggt cactaaccct ggcttatgtg aggaagcgat
     1261 gattatctat ttacacggtt ttgactctaa cagtccgggt aaccacgaga aagtcttaca
     1321 attgcagttt attgacccgg atgtacgctt gataagctac agtacgcggc atccgaaaca
     1381 tgatatgcag catctgctta aagaagtgga caaaatgttg caactgaacg ttgacgagcg
     1441 tccgctaatt tgcggcgttg gcctgggcgg atactgggcg gaacggattg gttttctttg
     1501 cgacatccgc caggtgatct tcaaccctaa tttgttccct tatgagaaca tggaaggcaa
     1561 gattgatcgc ccggaagagt atgccgatat tgccactaag tgtgtgacca acttccgcga
     1621 gaagaatcgc gatcgttgtc tggttatttt gtcgcgtaat gatgaagcac ttaacagcca
     1681 gcggacatct gaagagttgc atcattatta cgagattgtc tgggacgaag agcagacgca
     1741 caaattcaag aatatctccc cgcatttaca gcgcattaaa gcgttcaaaa ccctcgggta
     1801 aatgccctcg tcgcatcagg taaccttgcc ggtacctgat gcgctccgaa t
//
LOCUS       DAEVZA010000307         1795 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEVZA010000307 DAEVZA010000000
VERSION     DAEVZA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.309"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(14..1057)
                     /locus_tag="LAF92_005193"
     CDS             complement(14..1057)
                     /locus_tag="LAF92_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBI9794063.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(1183..1758)
                     /gene="nudK"
                     /locus_tag="LAF92_005194"
     CDS             complement(1183..1758)
                     /gene="nudK"
                     /locus_tag="LAF92_005194"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBI9794064.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
BASE COUNT          435 a          509 c          417 g          434 t
ORIGIN      
        1 cccgcaattt tgtttaacgg gtgatccaca acgtgggcca ggcgtctggc ccatgccagt
       61 tatcgcaggt gggttctgcg gcataacgca ccaggcgaaa acgctgaccg tcaaagcgcc
      121 agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc accagttcac
      181 gcgatttctc atcaaatgtt gcgttcatca gttccagttc attcgtctcc tgaccgttgt
      241 tgaatggcaa acgcaaccga accgggcgcg aggctagtgg ctttttacgc gacacaatcc
      301 atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc agcgctttat
      361 catcagtcag cgcagtgaca ttcacctcac gacgtaacgg atcaagcgag cagcgcagac
      421 cattcatccg ccagtttcca taatccagca aatcgttgcg ttcttcgagt gagagtggcg
      481 tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg cgcgggcggt acgctgagcg
      541 gcggttcgtc ccctttcttg atccacgcgg tttcactgcc aacgcgcttt tgctgagcat
      601 caataaacaa caacgccgct tttaagccac tcagagaaat ggtctgattg ccgtcgcgta
      661 aggtgatcgc cttcccttcc tgaatcatct gcaaaaacgc ggtgatggtt gccgtatcat
      721 cggttactaa taaccatggt gaaatccgcc acttgtcacc acttaacgcc aacggctcac
      781 catctaacag cagccgtggc gctatctctc cttcagacgc atccggcgac ttcaacccgc
      841 cgcgctcaat acgtaaaacg gcatcggtat gcgccccggc gctgcggctc agggtcatca
      901 ccagtccatt atgatcgccc gtattgcgcg ccacgcagaa gttttggtta ttacaggtga
      961 cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac gtcggcaaca
     1021 aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga cggtacgacc ccagaagttc
     1081 aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gcggctcaat cagatttgtc
     1141 ggattgagcc aataataaac aacgaaatat tattgttatt tttcagtcca ttaaatgtga
     1201 cgtttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc cggttttgat
     1261 catctccagc gcctggctga acggcagctc gagcacttca atatcttcat cttcaacacc
     1321 gccaccggcg ttagcgcgct gattgtcact gtattcggcg ataaaaaagt ggattagctc
     1381 agtcacaccg ccaggcgaca tatacagttc aaataatttg cgcacttcgc ccacttcata
     1441 gcccgtctct tcaatcgctt ctttacgaat gcacacttcc ggttcgtcgt tatccagcag
     1501 cccggcgcag gtttcaatca gctgcccgct ttcattgcca ttaacccagg tagcgacacg
     1561 gaactgacga atcagaacca cgctcttttt cttcgcgttg tacaggagga tcgtcgcacc
     1621 attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt tgcgcgtgag
     1681 atcgtaggta atgttgtgca gggtgaaata gttatcggag agaattttgt ctttaatgag
     1741 ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg aacaattatc ttacg
//
LOCUS       DAEVZA010000308         1794 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEVZA010000308 DAEVZA010000000
VERSION     DAEVZA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.310"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..609
                     /locus_tag="LAF92_005195"
     CDS             <1..609
                     /locus_tag="LAF92_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794065.1"
                     /translation="DVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGS
                     NQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGY
                     SVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHL
                     EGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            685..>1794
                     /locus_tag="LAF92_005196"
     CDS             685..>1794
                     /locus_tag="LAF92_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI9794066.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPTKK
                     DSPNYGSPVNPLLGAKVLPVETDLALPGPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESFWLARGGVLKQHKGHPLA
                     RLWRALPEAVRLSPHTYMMAVSTTGQWLILGWPERVPEADEVPPPEPPAYRVLTGVVD
                     GFGRSLIFHREAAGELAGEITGVTDGAGRRFHLALSTQAQRAEAFRKQRVTSLSSPAG
                     PRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGE
                     LRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT          398 a          475 c          580 g          341 t
ORIGIN      
        1 gatgtgataa acaaccatgc cgaaaaaata ggtaacaacc aggcgatcac cgttaccaat
       61 aaccagatcc agaacattgg cgttaatcag atacagacgg ttggtgtcaa ccaggtggaa
      121 acggtgggca gtaaccagat tatcaaagtg ggatcaaacc aggttgaaaa ggtggggatc
      181 attcgtgcgc tgacggtggg tgtagcttac cagacgacgg taggcggcat tatgaatacc
      241 tcggtggcgt tgttgcagtc ctcacaggta gggctgcata aatcactgat ggtgggaatg
      301 ggctacagcg tcaatgtggg gaataacgtc accttctcgg tgggcaagac gatgaaggaa
      361 aacaccggac aaacagcagt ttattctgcc ggtgagcatc ttgaactctg ctgtggtaag
      421 gcaaggctgg tgctgacgaa ggacggaagc atatttctca acggtacgca cattcatctg
      481 gaaggggagt cggatgtgaa cggtgatgcg ccagtgatta actggaactg tggtgccaca
      541 caacctgtac cggatgcgcc tgtgccgaaa gatttacccc ccggaatgcc ggatatgcgg
      601 caattttgag tcaggtgcaa aaaattaaaa cggaactggt gcagtgacca gatgaaccat
      661 attaaaagca gaaggaattg atttatgggc ggcggaaaac cggcggcgcg gcagggtgac
      721 atgacccgca agggactgga tattgtgcag ggttcagcag gggtgctgat aggtgcgcca
      781 accggtgtgg cctgctcggt gtgtcccaca aaaaaggaca gtccgaacta tggcagcccg
      841 gtgaacccgc tactcggtgc gaaggttctg cctgttgaaa ccgacctggc actgcccggc
      901 ccgctgccgt ttattctttt ccgcgcctac agcagctacc ggaccaggac gcccgcgcca
      961 gtgggcgtgt ttggtcccgg ctggaaagcg cccttcgata tccgcttaca gatacgcgat
     1021 gaaggcctga tactcaacga cagcggtggt aggagcattc actttgagcc gttgtttccc
     1081 ggcgagataa gctacagccg cagcgagtcg ttctggctgg caaggggcgg agtgctgaaa
     1141 caacacaaag gccatccgct tgcgcggctc tggcgggcgt tgccggaagc ggtccgtctg
     1201 agtccacaca cctatatgat ggctgtcagc acaaccgggc agtggctgat actcggctgg
     1261 ccggagcgtg tgccggaggc ggacgaggtg ccgccgccgg agccgcccgc ataccgggtg
     1321 ctgacgggcg tggtggacgg cttcgggcgc tcactgattt tccaccgcga ggccgcaggc
     1381 gagctggctg gcgaaatcac cggggtgacg gacggcgcgg gccgccgttt tcacctggca
     1441 ctgagcacac aggcgcagcg ggcggaagcc ttccgtaaac agcgcgtcac gtctttatcc
     1501 tcccctgccg gtccccgttc tgtttcctct tctcaggttt tccccgacac gctgcccgcc
     1561 ggtacagagt acggtgccga taacggtatc cggctggagg cggtgtggct gacacacgac
     1621 ccggcctacc cggatgaaca gccgactgcg ccgctggcgc gctacacgta cacggccagc
     1681 ggagaactgc gggcggtgta tgaccgcagc gggacgcagg ttcgcgggtt tacttatgat
     1741 gcggagcacg ccgggcggat ggtggcacac cattatgcgg ggaggccgga gagc
//
LOCUS       DAEVZA010000309         1734 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEVZA010000309 DAEVZA010000000
VERSION     DAEVZA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.312"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(32..217)
                     /locus_tag="LAF92_005197"
     CDS             complement(32..217)
                     /locus_tag="LAF92_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794067.1"
                     /translation="MIIKIIIAFSICAMLIFSSIYKKELLSRNYIACSGIPSGWMPGL
                     ATKYVKEKTSCEKNGNN"
     gene            complement(1034..1507)
                     /locus_tag="LAF92_005198"
     CDS             complement(1034..1507)
                     /locus_tag="LAF92_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794068.1"
                     /translation="MGDHSAIYFEVSGDSSSPYLYDPNGSYNPYKKLENGNGQRGSGF
                     VFSGEFAKLDDFISYQENPNEVTQNKLDSEWFPYKVKLLSLPVTNDELLTIKNAALAL
                     DDGDEEPGSFSCAEWSSKLLKYIIPELDIYTLPNSLWEAVVKEKEKRIKLNGENK"
     gene            complement(1555..1734)
                     /locus_tag="LAF92_005199"
                     /pseudo
     CDS             complement(1555..1734)
                     /locus_tag="LAF92_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378345.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
BASE COUNT          608 a          339 c          201 g          586 t
ORIGIN      
        1 ttagataaag tattacaatc tcatcattga tttaattatt gccatttttt tcacatgatg
       61 ttttttcttt aacatatttc gttgccagac ccggcatcca cccagacggg ataccactac
      121 aagcaatata atttctagat aataattctt ttttgtaaat tgaagaaaaa atcaacattg
      181 cgcaaataga gaaagcaata ataatcttaa ttatcaacca aaggcgcacc attgttactt
      241 tagataatcg tcgcccacta atagcaaaaa acaaaatctc aaacgacatt acatataatg
      301 taaaaggtaa aaagataatt aacatatacc cccagctata tacaataata tcattccata
      361 aaaatagttc ataagtagca taacagccaa aaaaaacaaa agttgtggat aaaaaaatat
      421 aaatatggct ccgcaagtta actttagctt ttcttttaat atatttatag tcatatctat
      481 ttcaattccc atttgctcac attgtttagt ttatctttta aatactcctt cacttcatgt
      541 tccattactt ccttcccttt aataagcatg tgttttgccc acatggctcc caaatccctt
      601 cttcaatctc tctggctttc ttaacattat tttattattt acttgatcac taacttccct
      661 gagcatctaa taaataaaac aaagtaagaa aaataatacc atcccattag caatgtgttt
      721 gatattaacg caagcacctc tagattatta taagcatcta cacccctgaa tattatatct
      781 gtcaataata aagcaatgaa tgataataga ccacctgtac aacacccaaa gaacaaagaa
      841 taaaaaacat ttaatttata aaaaatatca gtaacaaata aaataacaaa ggaaaatgaa
      901 aggtataaaa ttaggtaaga ataccagtaa aaatcatgaa gttgatgttt ggcgtcaact
      961 ttataaacaa ggtaagcatt aacaaataat gatataataa aaaataaaag taatactaca
     1021 aatctaaata ttttcattta ttctcgccat ttaactttat tcttttttct ttttctttaa
     1081 caaccgcttc ccatagtgaa tttggcagcg tataaatatc aagttctggg attatatatt
     1141 ttaacaattt tgaagaccat tcggcacaac taaaactccc cggttcttca tcgccatcat
     1201 ctaatgccaa tgcagcattc tttatcgtta ataactcgtc attcgttacc ggtaaagata
     1261 ataactttac cttataggga aaccattcgc tatccagctt gttctgcgtt acttcattcg
     1321 gattttcttg ataactgata aaatcatcca gttttgcaaa ttcaccacta aatacaaaac
     1381 cggatcctct ctgcccattt ccattttcta attttttata aggattatag cttccattag
     1441 gatcataaag ataagggctt gatgaatcac cagacacttc aaaataaata gctgaatgat
     1501 ctcccattac ctcggcatta gtactatata ctctaattgt ggatgtaatt aaaattattt
     1561 ctgatgtata ttttgttttt ttctgagtgt tatctcctcg aacatccgtc cattctttat
     1621 ttaacctatt ctgatctatt cctgctatct ggttatctgt tcctgaacct tctttggtct
     1681 catacgtatc atccatcatt gacgcaataa atcgcgcctt acacgggcaa ctgc
//
LOCUS       DAEVZA010000310         1730 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEVZA010000310 DAEVZA010000000
VERSION     DAEVZA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.313"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            6..521
                     /locus_tag="LAF92_005200"
     CDS             6..521
                     /locus_tag="LAF92_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HBI9794069.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELM
                     KAVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVW
                     ISGETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRR
                     ATGWQRVETRA"
     gene            626..1168
                     /locus_tag="LAF92_005201"
     CDS             626..1168
                     /locus_tag="LAF92_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794070.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKN
                     YSVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVL
                     QAIEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFRAENGLNGKVTEQGDSYDL
                     QIRGLSIDQLVKITGYLKQL"
     gene            1165..1413
                     /locus_tag="LAF92_005202"
     CDS             1165..1413
                     /locus_tag="LAF92_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794071.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYD
                     AVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNET"
     gene            1403..1630
                     /locus_tag="LAF92_005203"
     CDS             1403..1630
                     /locus_tag="LAF92_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBI9794072.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQ
                     NSQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
BASE COUNT          590 a          298 c          416 g          426 t
ORIGIN      
        1 aaaaaatgaa tatccaggaa gcattaaacg tttttggatt atccggcgaa ttaactgaaa
       61 aagatatcaa agcagcatac agaaaagccg ctttaaaata tcatccagat cgtaacccgt
      121 taggggctga actgatgaaa gcggtaaatg cagcttttga tgtattgatg gcaaatattg
      181 ataaaataaa tcagttccag agcactgatg aacatgcacg atataattac ggtgatgacc
      241 tggaaaaagt attaaacgtt ctttctggtt tatctggtct ggtatttgaa gtgataggta
      301 actgggtctg gattagtgga gaaaccatta cacataagga aactttaaaa gaaatcgggt
      361 gtaaatgggc ggcaaagaaa aaacaatggt tttatcgtcc agacgaacat aaaagttact
      421 ggaatcgtga agaacacacg atagaagaaa tccgcgcaaa atacggtaca accggacagc
      481 gcagggcgac agggtggcaa cgcgtggaaa ccagagcgta accagaacgg gggcgagaag
      541 cccccgatat tatagccaac ccgtcttttg gggcatcgaa cccaaaaaga cgggaacagc
      601 agccacgtag caaaaaggga aacagatgaa ctcttttttt gaacaatatc accctgtctt
      661 tgaagttgtc tgccgtatcc tggggaacgg ctggcgcgtg aacaaacttg atgattgttc
      721 gtctcgtata aaactgacgt caccgcagtt taaaaattac tctgtgcata ttcggatgga
      781 aaaagaccgg ttttctgttg tggggagtgt ggatagtcgc tcctggcgta gtccgcatca
      841 tgtttgcacg ttatccagaa aacggaatcc cgttgatatt gcagccgata tcgagcgaaa
      901 aattctggta aatgcttcgc aggaggtgtt acaggcgatt gagtatgaga aacaccaagt
      961 ggaaaaaaag gacgaaattc tgatcctgaa aggtatgtta tcgcaacttg tccagcttga
     1021 aagctggtat ggggcattga caggctttag agctgaaaac ggattgaacg gtaaagtcac
     1081 cgaacagggc gacagttatg atttacagat tcggggcttg agtatagatc aacttgttaa
     1141 aattacaggg tatttgaaac agttatgaga aaaaataaag gcgatgtgac ttattttctt
     1201 gaaaaagaag gtgacaatta caggcttaca aaaaggataa aagccagaac aaatgttaaa
     1261 atcgggaaca aaactacgaa aattacattg tatgatgccg tactgaatga gaatgaatta
     1321 caacatattg attttacgtg tgccggatta agggaagacg atgagacgcc agttaagaat
     1381 ttaattaagg agtttatgtt aaatgaaacc tgataataca cctgaaaatg taaaaaaatt
     1441 acgtttaaaa gcaggattaa cacaaaagga atgcagtcat atttatggtg ttgggctaag
     1501 aacctggcag aaaaaagagg aagttaatac tcaaaacagt caaagtctgt cactggttga
     1561 gtttgagttt cttttgttac ttgcaggaga acatcctgaa tacgtgctct gcaaaaggga
     1621 gtcaaaataa aatgacctgt gatggtttta tattagttcc tggagttgtg cccgtagctc
     1681 gctgacgctc acacgcgcag tacagcgccc cgccatgctg caatgcacca
//
LOCUS       DAEVZA010000311         1730 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEVZA010000311 DAEVZA010000000
VERSION     DAEVZA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.314"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>1730
                     /locus_tag="LAF92_005204"
     CDS             <1..>1730
                     /locus_tag="LAF92_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HBI9794073.1"
                     /translation="AAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKF
                     YEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQ
                     YLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQL
                     LPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESS
                     PGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGG
                     RGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLR
                     IEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTL
                     NIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFI
                     LDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTA
                     AIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGK
                     FRPLRMPGKP"
BASE COUNT          327 a          592 c          540 g          271 t
ORIGIN      
        1 ggcagccgtg cagaccctgc gtgagatgaa cgccgacaac ctgcgcaagg tgccggccga
       61 tgcacccacg gccttcatca agccgcgctg gaagccgctg gtgatcaccc cggaaggcct
      121 cgaccggaaa ttctacgaaa tctgcgccct gtccgagctg aagaacgccc tgcgctccgg
      181 cgacatctgg gtcaagggct cgcggcagtt ccgcgacttc gacgactacc tgctgccggc
      241 cgagaagttc gccgcactca agcgcgagca ggccctgccc ctggcgatca acccgaacag
      301 cgaccagtac ctggaagagc gtttgcagct gctggacgag cagttggcca ccgtcacccg
      361 cctggccaag gacaacgagc tgcccgatgc catcctcacc gagtcagggc tgaaaatcac
      421 cccgctggat gcggcggtgc cggatcgggc gcaggcgctg atcgaccaaa ccagccagtt
      481 actgccgcgc atcaagatca ccgaactgct gatggacgtg gacgactgga cgggcttcag
      541 ccgccacttc acccacttga aggacggggc cgaggccaaa gacaggacgt tgctgctgtc
      601 cgcaatcctc ggtgatgcga tcaacctcgg gctgaccaag atggccgagt cgagccccgg
      661 cctgacctac gccaagctgt cctggctgca agcctggcac atccgcgacg aaacctattc
      721 ggcggccttg gccgagctgg tcaaccacca gtatcgccac gcctttgccg cccactgggg
      781 cgacggcacg acctcatcct ccgatggcca gcgcttccgc gcgggtggcc ggggcgagag
      841 caccgggcac gtcaacccga agtacggtag cgagccggga cggctgttct atacccatat
      901 ctccgaccag tacgcgccgt tcagcacccg cgtggtgaat gtcggcgtcc gcgattccac
      961 ctatgtgctc gacggcctgc tgtaccacga gtccgacctg cggatcgagg agcactacac
     1021 cgacacggcc ggcttcaccg atcacgtctt tgccctgatg cacctgctag gcttccgctt
     1081 cgcgccgcgc atccgcgacc tcggcgaaac caagctgtac gtgccgcagg gcgtgcaagc
     1141 ctacccgacg ttgcgcccgc tgatcggcgg caccctgaac atcaagcacg tgcgtgccca
     1201 ctgggacgac atcctgcgcc tggccagctc gatcaagcag ggcaccgtca ccgcctcgct
     1261 gatgctgcgc aagctcggca gctacccgcg ccagaacgga ctggccgtgg ccctgcgcga
     1321 gctgggccgg atcgagcgca cgctgttcat cctggactgg ctgcaaagtg ttgaactgcg
     1381 ccgccgcgtg catgccggcc tgaacaaagg tgaggcgcgc aactcgctgg ccagggcggt
     1441 gttcttcaac cgccttgggg aaatcaggga tcggagcttc gagcagcagc gctaccgggc
     1501 cagcggcctc aacctggtga cggcggctat cgtgctgtgg aacacggtgt acctggaacg
     1561 cgccacccag gggttggtcg aggccggcaa gccggtggac ggcgagctgc tgcaattcct
     1621 gtcgccgctg ggctgggagc acatcaacct aaccggcgat tacgtctggc ggcagagccg
     1681 cagactggaa gacgggaagt ttcggccctt acggatgccc ggaaaacctt
//
LOCUS       DAEVZA010000312         1713 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEVZA010000312 DAEVZA010000000
VERSION     DAEVZA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.315"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>1713
                     /gene="traD"
                     /locus_tag="LAF92_005205"
     CDS             <1..>1713
                     /gene="traD"
                     /locus_tag="LAF92_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBI9794074.1"
                     /translation="QYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLI
                     TFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDS
                     EIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVIYDRSCEFVKSYYDPSIDKILNPL
                     DARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDPFWQGSGRTIFAEAAYLMRNDPNR
                     SYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKAIRYLQGI
                     EHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVVSMWLSIAIRGLLAMGEN
                     RNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLF
                     DVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQYSYGADPVRDGISTGKEMERQTL
                     VSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARPKVAPEFIPRDINPEMENRLSAVL
                     AAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPQQPQQPVSPAINDKKS
                     DSGV"
BASE COUNT          405 a          436 c          478 g          394 t
ORIGIN      
        1 tccagtatta cggcaaaacc ttccggatga acgcggctca ggtactgcat gataaataca
       61 tgatctggtg cggtgagcag ctatggtctg cattcgttct ggcgtctgtc gtggccctgg
      121 ttatttgcct gatcaccttc tttgttgtct cctggattct ggggcgtcag ggtaaacaac
      181 agagcgaaaa tgaagtcaca ggtggtcgtc agctgacaga caatccgaaa gacgttgccc
      241 ggatgctgaa aaaagacggc aaggactccg atatccggat tggcgacctg ccgattatcc
      301 gggattccga aatccagaac ttctgtctgc acggaacagt gtcgacgggt aagtcagagg
      361 ttattcgacg tcttgccaac tatgcccgta aacgtggcga catggtggtg atttatgacc
      421 gttcgtgtga gtttgttaaa agttactatg atccctccat cgataagatc ctgaatccgc
      481 tggatgcccg ctgtgccgcc tgggatttgt ggaaggagtg cctgacacag ccggattttg
      541 ataatgtcgc gaacacgctg atcccgatgg gaacgaaaga ggacccgttc tggcagggat
      601 caggacgtac catttttgcg gaagcggcgt atctgatgcg taatgacccc aaccgcagct
      661 acagcaaact ggtggacaca ctgctttcca tcaaaatcga aaaactgcgt accttcctgc
      721 gtaattcacc ggcggccaac ctggtggaag agaaaattga gaaaacggcg atttccatcc
      781 gtgctgtgct gaccaactac gtgaaagcca tccgttacct gcaggggatt gagcataacg
      841 gtgaaccctt caccatacgt gactggatgc ggggtgtccg ggaagatcag aaaaacggct
      901 ggctgtttat ctcgtcaaat gccgacactc atgcctccct gaagccggtg gtttccatgt
      961 ggctgtccat tgccattcgt gggctgctgg caatgggaga aaaccgtaac cgtcgtgtgt
     1021 ggtttttctg tgatgagtta cccacgttac acaaactgcc ggacctggtg gagatcctgc
     1081 cggaagcccg taagttcgga ggctgttatg tgtttggtat ccagtcctat gcccagctgg
     1141 aagatatcta cggtgagaaa gcggcggcca cgctgtttga tgtgctgaat acccgtgcct
     1201 ttttccgctc cccgagccat cagattgcag agttcgcggc cggtgagatt ggtgagaaag
     1261 agcacctgaa agccagtctg cagtattcct acggtgctga tcccgtgcgt gacgggatat
     1321 caaccggtaa ggagatggag cgccagacgc tggtcagtta ttccgacatt cagtctctgc
     1381 cggatctgac ctgttatgtc actctgcccg gaccgtatcc ggcagtaaaa ctctcactga
     1441 aatatcaggc acgtccgaag gttgcccctg agtttattcc gcgtgacatc aacccggaaa
     1501 tggagaaccg tctgagtgcc gtacttgccg caagggaagc agaaggtcgt cagatggcca
     1561 gcctcttcga accggatgtg ccggaagttg tttccggaga agacgtgact caggctgaac
     1621 agccgcaaca gccgcaacag ccgcaacagc cgcaacagcc gcaacagccg gtgtctcctg
     1681 ccatcaacga taagaagtca gattcaggtg tga
//
LOCUS       DAEVZA010000313         1671 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEVZA010000313 DAEVZA010000000
VERSION     DAEVZA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.317"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>1671)
                     /gene="tkt"
                     /locus_tag="LAF92_005206"
     CDS             complement(<1..>1671)
                     /gene="tkt"
                     /locus_tag="LAF92_005206"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBI9794075.1"
                     /translation="TGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDG
                     CLMEGISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIH
                     EIDGHDPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVAL
                     ARQKLGWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMS
                     GGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLT
                     IWKGSVSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAAR
                     MAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWK
                     LAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEME
                     ITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWY
                     KYVGLKGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
BASE COUNT          350 a          514 c          403 g          404 t
ORIGIN      
        1 aggcaccttt cactcccagc accttatgcg cttttgccac aatattctcg gcggtaaagc
       61 caaagaacgg gaacagctta tccgccggag cagattcccc gtaacccgtc atcccgacaa
      121 ttgccccttt cagaccaaca tacttgtacc agtaatcggc aatacctgct tccaccgcca
      181 cgcgagccgc aacgttagaa ggcaacaccg actcccgata ttcctcatcc tgggcgtcga
      241 aaatatcggt cgagggcagg gaaactacgc gtacattgcg accttctcct gctaatttct
      301 ctgccgcttg cagggtaatt tccatctctg aaccggtggc aatcagaata atatctggct
      361 taccgccgct gtctttcagc acataaccgc cacgagcaat ctctttaacc tgatccggcg
      421 tacgttccac ctgggccaga ttctgccttg agaggatcag tgccgtcggt ccgttgtggc
      481 gctcaaccgc cagcttccag cccaccgccg cttccacctg atcgcacggt cgccaggtgc
      541 tgaaatttgg cgttaagcgc aggctggcca gttgctcaac agcctggtgc gtcggaccat
      601 cttcgcccag gccaattgag tcgtgggtat aaaccataat ctgccgcgct ttcatcagtg
      661 ccgccatccg cgcggcgtta cgggcgtatt caacaaacat caggaacgtc gcggtatacg
      721 gcacaaagcc gccgtggtgc gcgatgccgt tggcgatagc ggtcatgcca aattcacgca
      781 ccccgtagtg aatgtagttg cccgctggat cttccttcag cgaaacagaa cctttccaga
      841 tggtcaggtt gctgggagcc agatccgccg aaccgccgag caactcaggc agcatcggcc
      901 cgtaagcgtt aagcgtattt tgcgaagcct tacgggtagc gattttcgcc ggatttgcct
      961 gtaactcatt gatatatttc tgagtcgttt tctcccagtc cttcggtaaa ccaccgctca
     1021 tccgtcgggt aaactcttct gccagttgcg gatgagcctt tttataggcg gcaaacttct
     1081 cattccagct ctgctgcgct ttttcgcctt tttcacgggc atcccaggcg tgataaatct
     1141 ttttagggat ctcaaatggc ggatggtgcc agcccagttt ttgccgtgcc agcgccactt
     1201 cttcttcccc cagtggtgcg ccgtgcgcct cttccttacc tgctttattc ggcgaaccaa
     1261 agccaatcac cgtacggcag ataatcagcg acggcttatc tttcacgctt tgcgcttcaa
     1321 ggatcgcttc cttcaccgcc tgcggatcgt gaccgtcgat ttcatggatc acatgccagt
     1381 gataggcttc aaaacgtttt gccgtatcgt cggtaaacca gccttctgtt tcaccgtcga
     1441 tggaaatacc gttgtgatcg taaaaaccaa tcagcttgcc cagtcccagc gtgcctgcca
     1501 gcgaacagac ttcgtgggaa ataccttcca tcaggcagcc gtcgcccata aacacatagg
     1561 tgaagtgatc gacgatctca tggtctggct ggttaaactg cgccgccaat gtacgttccg
     1621 ctatcgccag cccgacggcg ttcgccaaac cttgtccaag cgggccggtg g
//
LOCUS       DAEVZA010000314         1613 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEVZA010000314 DAEVZA010000000
VERSION     DAEVZA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.318"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>1613
                     /gene="rsxC"
                     /locus_tag="LAF92_005207"
     CDS             <1..>1613
                     /gene="rsxC"
                     /locus_tag="LAF92_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBI9794076.1"
                     /translation="TPLRQVPLAQRFVIPLKQHIGAEGELCVSVGDKVLRGQPLTRGR
                     GKMLPVHAPTSGTVTAIAPHSTAHPSALAELSVIIDADGEDCWIPRDGWADYRTRSRE
                     ELIERIHQFGVAGLGGAGFPTGVKLQGGGDKIETLIINAAECEPYITADDRLMQDCAA
                     QVVEGIRILAHILQPREILIGIEDNKPQAISMLRAVLADSNDISLRVIPTKYPSGGAK
                     QLTYILTGKQVPHGGRSSDIGVLMQNVGTAYAVKRAVIDGEPITERVVTLTGEAIARP
                     GNVWARLGTPVRHLLNDAGFCPSADQMVIMGGPLMGFTLPWLDVPVVKITNCLLAPSA
                     NELGEPQEEQSCIRCSACADACPADLLPQQLYWFSKGQQHDKATTHNIADCIECGACA
                     WVCPSNIPLVQYFRQEKAEIAAIRQEEKRAAEAKARFEARQARLEREKAARLERHKSA
                     AVQPAAKDKDAIAAALARVKEKQAQATQPIVIKAGERPDNSAIIAAREARKAQARAKQ
                     AELQQTNDAATVADPRKTAVEAAIARAKAR"
BASE COUNT          377 a          426 c          458 g          352 t
ORIGIN      
        1 acacccctgc gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt
       61 ggcgctgaag gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt
      121 acccgtggtc gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct
      181 attgcgcccc actctacggc tcatccttca gctttagctg aattaagcgt gattattgat
      241 gccgatggtg aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt
      301 cgcgaagagt taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga
      361 ttcccgacag gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac
      421 gcggctgagt gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct
      481 caggtcgtag agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc
      541 ggcattgaag ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct
      601 aacgatattt ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta
      661 acctacattc tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta
      721 ttaatgcaaa acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg
      781 attaccgagc gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg
      841 gcacggctgg ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc
      901 gatcaaatgg tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc
      961 ccggtcgtaa agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca
     1021 caggaagaac aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt
     1081 ttgccgcaac agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat
     1141 aacattgctg attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc
     1201 ctggtgcaat atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag
     1261 cgcgccgcag aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa
     1321 gcggctcgcc ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat
     1381 gcgattgctg ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt
     1441 gtgattaaag cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt
     1501 aaagcgcaag ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt
     1561 gctgatccac gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcg
//
LOCUS       DAEVZA010000315         1612 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEVZA010000315 DAEVZA010000000
VERSION     DAEVZA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.319"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..719)
                     /gene="metN"
                     /locus_tag="LAF92_005208"
     CDS             complement(<1..719)
                     /gene="metN"
                     /locus_tag="LAF92_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBI9794077.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQK"
     gene            907..1482
                     /gene="gmhB"
                     /locus_tag="LAF92_005209"
     CDS             907..1482
                     /gene="gmhB"
                     /locus_tag="LAF92_005209"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBI9794078.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT          410 a          390 c          418 g          394 t
ORIGIN      
        1 aacttctgcg ccagcggcgt tttcggatgc gagaacactt cacttaccgt gtcctgctcg
       61 atcagttcgc cattgctgat gaccgccacg caatcacaaa tacgcttcac aacgtccatt
      121 tcgtgggtga tcaacagaat cgtcaaaccc agacggcggt taatgtcttt cagcagttcg
      181 agaatagaac gtgtcgttgc cgggtccagc gcgctggtgg cttcatcaca cagcaatact
      241 ttgggattgc tggctaatgc acgggcaatc gccacacgtt gtttctgccc accggaaaga
      301 ttcgacgggt agctatcatg cttatcgcca agaccaacca atgacagcaa ttccgtcacg
      361 cgacgtttga tctcgtcttt cggtgtgttg tccagctcca gcggcagagc cacgttgcca
      421 aaaacagtac gcgaagagag caggttaaaa tgctggaaaa tcataccaat ctggcggcga
      481 gctttggtca actcggattc tgacagcgtg gtcagttcct ggccatcgac cagcacgcta
      541 ccctcggttg ggcgctccag caggtttaca caacgtataa gcgtactctt acccgcgcct
      601 gaggcaccga taacgccata aatttgtcca gctggcacat gcaggctgac gttgttcaac
      661 gcctggatgg tgcgggtgcc ctggtggaac actttggtga tattcgaaag ttttatcatt
      721 gattatttat tatcgtcatt aagttagtcg tggcatctcg aatgcctgaa acgggcaacg
      781 ccgtcaatga aatggatgtt aaggcatcca gacgtctaaa tcaatcaggt ttatgcgaag
      841 agcactttct tgcaggtcga aacatgcgat actagcgtcc catgccttat taaggagcta
      901 taaaaggtgg cgaagagcgt acccgcaatt tttcttgacc gtgatggcac cattaatgtc
      961 gatcacggct atgtccatga gatcgacaac tttgaattta tcgacggtgt tattgacgcc
     1021 atgcgcgagc taaaaaaaat gggctttgcg ctggtggtag taaccaacca gtctggcatt
     1081 gctcgcggta aatttaccga agcacagttt gaaacgctga ccgagtggat ggactggtcg
     1141 ctggcggacc gagatgtcga tctggatggt atctattatt gcccgcatca tccgcagggt
     1201 agtgttgaag agtttcgcca ggtctgcgat tgccgcaaac cacatccggg gatgcttttg
     1261 tcagcacgcg attatttgca tattgatatg gccgcttctt atatggtggg cgataaatta
     1321 gaagatatgc aggcagcggt tgcggcgaac gtgggaacaa aagtgctggt gcgtacgggt
     1381 aaacctatta cacctgaagc agaaaacgcg gcagattggg tgttaaatag cctggcagac
     1441 ctgccgcaag cgataaaaaa gcagcaaaaa ccggcgcaat gattaaaaga tgagcggttg
     1501 aaataaaaat gcatttttcc gcttgtcttc ctgagccgac tccctataat gcgcctccat
     1561 cgacacggcg gatgtgaatc acttcacaca aacagccggt tcggttgaag ag
//
LOCUS       DAEVZA010000316         1611 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEVZA010000316 DAEVZA010000000
VERSION     DAEVZA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.320"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            136..546
                     /gene="fliS"
                     /locus_tag="LAF92_005210"
     CDS             136..546
                     /gene="fliS"
                     /locus_tag="LAF92_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBI9794079.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            546..911
                     /gene="fliT"
                     /locus_tag="LAF92_005211"
     CDS             546..911
                     /gene="fliT"
                     /locus_tag="LAF92_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBI9794080.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            989..>1611
                     /gene="amyA"
                     /locus_tag="LAF92_005212"
     CDS             989..>1611
                     /gene="amyA"
                     /locus_tag="LAF92_005212"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBI9794081.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFR"
BASE COUNT          443 a          371 c          415 g          382 t
ORIGIN      
        1 gtcgcgcgct ataaagaaca atttacccaa ctggacgttt taatgacctc gttaaatagc
       61 accagcagct acttaacgca gcagttcgaa aacaacagta attccaagta atcattattc
      121 atcgggagac aggtcatgta cgcggcaaaa ggcacccagg cctatgcaca aattggcgtc
      181 gaaagcgccg taatgagcgc cagccagcag cagctggtca ccatgctatt tgatggagtg
      241 ctaagcgcac tggttagagc gcgcctgttt atgcaggaca acaatcagca aggcaaaggc
      301 gtctctttgt caaaagcgat caacatcatt gagaacggac tgcgggtgag tcttgatgaa
      361 gagagcaaag acgaactaac ccaaaacttg attgctcttt atagctatat ggtcaggcgc
      421 ttgctgcaag ccaatttacg caacgatgtc tccgcagtcg aagaagtgga agcattaatg
      481 cgcaatattg ccgatgcctg gaaagaatcg ttactctccc cttctttgat tcaggaccca
      541 gtctgatgaa ccacgcaccg catttgtatt tcgcctggca acaactcgtc gaaaaaagcc
      601 agctcatgtt acgcctggca acagaagaac aatgggacga actcatcgcc agcgagatgg
      661 cgtatgtgaa tgcggtgcag gagattgcac atctgactga agagattgac ccgtccacca
      721 cgatgcagga gcagctccgc ccgatgctgc gcctgattct cgacaacgaa agcaaggtaa
      781 agcagttatt acagattcgg atggatgaac tggcgaaact ggtcggtcag tcatcggtgc
      841 aaaaatcggt gttaagtgcc tatggcgatc agggcggctt tgtgctggct ccgcaggata
      901 acctcttttg aatctgaacg agtcgatggc tcgcgaataa tccgattacg gctacgcttc
      961 taatgttccc cttgaatgga gtcgaagaat gcgtaatccc acgctgttac aatgttttca
     1021 ctggtattac ccggaaggcg gtaagctctg gcctgaactg gccgagcgcg ccgacggttt
     1081 taatgatatt ggtatcaata tggtctggtt gccgcccgcc tataaaggcg catcgggcgg
     1141 gtattcggtc ggctacgact cctatgattt atttgattta ggcgagtttg atcagaaagg
     1201 cagcatccct actaaatatg gcgataaagt acaattgctg gccgccattg acgccctgaa
     1261 acgtaatgac attgcggtgc tgttggacgt ggtagtcaac cacaaaatgg gcgcggatga
     1321 aaaagaagct attcgcgtgc agcgtgtaaa tgctgacgac cgtacgcaaa ttgatgaaga
     1381 aatcattgag tgcgaaggct ggacgcgtta caccttcccc gcccgtgccg ggcaatactc
     1441 acagtttatc tgggatttca aatgttttag cggtatcgac catatcgaaa accctgatga
     1501 agatggcata tttaaaattg ttaacgacta caccggcgaa ggctggaacg atcaggttga
     1561 tgatgaatta ggtaatttcg attatctgat gggcgagaat atcgattttc g
//
LOCUS       DAEVZA010000317         1575 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEVZA010000317 DAEVZA010000000
VERSION     DAEVZA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.321"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..549)
                     /locus_tag="LAF92_005213"
     CDS             complement(<1..549)
                     /locus_tag="LAF92_005213"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="catalyzes the formation of
                     selenocysteinyl-tRNA(Sec) from seryl-tRNA(Sec) and
                     L-selenophosphate in selenoprotein biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBI9794082.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGA"
     gene            complement(647..1255)
                     /locus_tag="LAF92_005214"
     CDS             complement(647..1255)
                     /locus_tag="LAF92_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBI9794083.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
BASE COUNT          382 a          436 c          400 g          357 t
ORIGIN      
        1 cgcgccgcca atctccacca gttcgccgcg agataccacc acctcttttc cgctggcagt
       61 ggccgccaac atcaataaca ccgccgccgc attgttattg acgatacagg catcttccgc
      121 ccccgtaata cggcacaaca gttgcgccag cgcccgatcg cgatgtccgc gtccggcgtc
      181 gtccagatcg tactcgaggg tcactggcga acgcatagcc tgcgcaacgg cttccaccgc
      241 ggcttccgcc tgtaaagctc gcccaaggtt ggtatgcagc acggttcccg tcaggttgat
      301 caccggacgc agcgcgctct gcgcttcttt cgtcaaccgg gcatcgactt cttgcgccca
      361 gttttcacac cacgcaggca gcgtctggct gccacgaatc acttctcgcg cttcgtcgag
      421 catctgacgc aacaattcca ccacgcgggt gtgaccataa gtatcacgca aagaaaggaa
      481 ggagctatcg cgcaataagc gatcaatagc cggaagttga ctatagaggg aacgcgtttc
      541 ggttgtcata ggaaacctgg ctgatcaagg ccctctcaca cggagaaggg cgtttaacat
      601 aaccacggat tgtaacgtga gatgggtcag gaggacatat cgcgcatcaa gcctttggcg
      661 gttcggtgcg agcaaaactt tcgcggctaa acaggttttc gaccagtttg actaagtgtg
      721 ggcggtcgac acaccaacct ggcgcaacgc ggcggaaatt gagatatccg acggcacagg
      781 caatggcgat agtcgccaga ttgaccgtat cggttttgag tgtgccatcg accagatatc
      841 cttccagcac atccagactg cggttgattt tctcccgctg gcgtaacaat tcatcttcag
      901 actgctgcgc cgctggacgc gcctgttcac gcaccgatac cagcccggca tccataatgc
      961 catccgccag tgcctcaatt ttgcgcaccc gcaacgactc cagcggatcg cgcggcaaca
     1021 tcgccggagc gacattcatt aattcaatat attcagcgat gatcggcgaa tcaaaccaac
     1081 attcgccctc ttcggtcacc agcaccggca cttttcctaa cgggttaaat tgcgccacgc
     1141 cgttgtccgc gttatagggc agttcattaa taaattcgaa agttatgccc ttttctaaca
     1201 acagaataga aagtttgcgt acaaacgggc tggtgtagct accgacgagt ttcatgccga
     1261 gtcctttgtg cgaggaaaaa tatcagtatg gcctggtgat gggaaaaagg gcagaaaatg
     1321 ttgatggttt gttacttcca aataaatcac atatttatca tggtgatata aatattttcc
     1381 taattgtttc tatctgatgg atatctcact taaggctttc ttataaatct gtagggtttt
     1441 gcctggaagc agacaaataa cccgataaaa caaggatgag cagatgagcg gaaaaccggc
     1501 agcgcgtcag ggcgacatga cgcagtatgg cggtagcatt gttcagggtt cagccggggt
     1561 gcgcattggt gcccc
//
LOCUS       DAEVZA010000318         1502 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEVZA010000318 DAEVZA010000000
VERSION     DAEVZA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.322"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..343
                     /locus_tag="LAF92_005215"
     CDS             <1..343
                     /locus_tag="LAF92_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBI9794084.1"
                     /translation="AWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVAPSI
                     IKENLSLHLTHTVKWTESLDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQRVMHC
                     RKAFSDAKVFK"
     gene            340..1326
                     /locus_tag="LAF92_005216"
     CDS             340..1326
                     /locus_tag="LAF92_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794085.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
BASE COUNT          366 a          286 c          347 g          503 t
ORIGIN      
        1 tgcctggcac acgcgtttga tggaagacgg cgttcaggca atgcgcgatt atcttgccgg
       61 actggatatc gcttcgccag agcatcaggt tctgatgaac gtaacagcaa aaagcgaggt
      121 cgctccgtca attatcaaag aaaatctctc attacactta acccatacgg tgaaatggac
      181 tgaatctctc gatacatttc tgaatatgcc aaccccggta gctttccttg aaataagtaa
      241 taagccctat ttggggaata tgttaaatga ttttgctggc gtggatcagc aacgagttat
      301 gcattgtcgt aaagcattta gtgatgcaaa ggtattcaaa tgattcctga ttatttaact
      361 tttattcgct ttcaggataa acgaaatctg atatacattt atgctattgg acttattctg
      421 ataggctttt attggaaaaa tgcaggattt acttttccat cagaggatat tggtgtcgtt
      481 agtgggattc tggccctggt gctgtataat tttatttttg atctcaaggc gtactgggct
      541 tataaatgcg tcacgaagaa tatcgatttt tcgtggttta agaaaaagca gaaccacaaa
      601 atagaattat ttcttacaca acctctggtg gcaggatttc tgtcgttaat catgttgagt
      661 gcaatgagtt gggggctata ccagcttcta ccctcgttat atgcgctgtt cctgatttcg
      721 ttacttgggc cgttggtcat ctttctgctg tttcggatga tccgcaccag ttatgtcaag
      781 caggtcgcta tttcagtagc gaaaaaagta aaatataaaa gtctgactcg ctatgtgctg
      841 ctttcggtgt gcatctcaac ggttgttaac ctgcttacta tcagcccgtt gcgtaacagt
      901 gattcttttg tgacagaggg gcagtggtta acgtttaaat cgataattgc attgctcatt
      961 ctttgtggcg tagtgttggc gattaatctg ttttttctgc gcttctccaa gcggtacgct
     1021 tttctgggca ggcttttttt gcaggaaatc gatctgtttt tctccagtga aaatgcgttg
     1081 tcgacctttt ttgccaagcc gctttggctt cggttattca tattgctggt tattgaaatg
     1141 atgtggatta cgctggtgtc ggtattggca acgcttgtgg aatggcggat ttggtttgaa
     1201 gcctattttt tactctgcta tgtaccgtgc ttaatttact attttttcca ttgtcgattc
     1261 ctctggcata acgattttat gatggcatgt gacatgtatt tccgttgggg gcattttaat
     1321 aagtgaggaa gttacaggaa gtgaccagat aacgcatata tgttctctac tatcttgcgc
     1381 attttgattg ttgactgagt aaccagacag ttgatgtgca caatttcccc tcgccctaac
     1441 agacgtgggc gggggcaccc ggtgtgtgca atatacgaaa aaaaagcccg tactttcgta
     1501 cg
//
LOCUS       DAEVZA010000319         1497 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEVZA010000319 DAEVZA010000000
VERSION     DAEVZA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.323"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..1211)
                     /locus_tag="LAF92_005217"
     CDS             complement(<1..1211)
                     /locus_tag="LAF92_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HBI9794086.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTIL"
     gene            complement(1305..>1497)
                     /gene="nuoB"
                     /locus_tag="LAF92_005218"
     CDS             complement(1305..>1497)
                     /gene="nuoB"
                     /locus_tag="LAF92_005218"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBI9794087.1"
                     /translation="CPPRPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSER
                     ERKRGERIAVTNLRTPDEI"
BASE COUNT          337 a          400 c          428 g          332 t
ORIGIN      
        1 agaatggtat tttgcagcgc cgctttctcg taagacgcca gacgtttcgg catccagtcg
       61 aggaactcac gcagcaggcg atcccagccg cgcggcaggt cgtgcgctac gccgccaata
      121 cggaaccacg ccgggtgcat acggaaaccc gtgatcgctt ccaccagatc gtaaattttc
      181 tgacgatcgg taaaggcgaa gaacacgggc gtcattgcgc cgacgtcctg aataaaggtc
      241 gagatgtaca gcaggtggct gttgatacgg aacagttcgg agagcataac gcgaatgacg
      301 ttaacgcgat ccggcacggt gatcccggcc agtttctcta ccgccagcac gtaaggcatt
      361 tcgttaacgc agccgccgag gtattcaata cggtcggtat acggaatgta gctgtgccag
      421 gactggcgct cgcccatttt ctccgcacca cggtggtggt agccgatgtc tggtacgcag
      481 tcgacaatct cttcgccgtc gagttgtaaa acgatacgga aagccccgtg cgccgacggg
      541 tggttcggac cgaggttgag gaacatgaag tcctcgtttt cggtgccgcg cttcatcccc
      601 cactcttccg gtttgaaggt cagggcttcc atctccagat cctgtttggc tttggtcagc
      661 tcaaacggcg agaattcggt agcgcgcgcc gggtaatctt tacgcagcgg gtgacctttc
      721 caggtttgcg gcatcatgat gcggcgcagg ttcgggtgac cgtcgaaagt aatgccaaac
      781 agatcccagg tttcacgctc ataccagtta gcgttcggga acagtttggt gaaggtcggt
      841 acgtgcaggt cgttttctgc cagcgccacc ttcagcatga tgtcgcggtt acgatcgata
      901 gaaatcagat ggtagaaaac ggaaaaatcc gcggcaggta acccttcgcg gtgtgtgcgc
      961 agacgttcgt ccatgccgtg taagtcaaac agcatgacgt aaggtttcgg cagtttcttt
     1021 aagaaatcgc caacttccag taattgttca cgcttgatcc acacaacggg aaccccggtg
     1081 cgagtcgcct gaacagtaaa ggcatccggc ccaaaacggt tgcgcagttc gccaatcacc
     1141 ggatcatcaa gatgatcgcg ggtctgccag gcgggttctt gcgcggttaa gtcggtcata
     1201 ttgttcacca ttgcaaatgg tccgtggtga ctgtcggcgc agtaagcttc gcgaaataga
     1261 tttaggaata agcgaagaaa aatccctttg ccgacaggcg caaattaaat ctcgtcaggt
     1321 gtacgcaggt tggttacggc aatgcgttca ccgcgcttgc gttcgcgctc tgattgcata
     1381 ttggcgcgat aaacgccctg atcgccaacc acccaggaga gcggacgacg ttctttgccg
     1441 atagattcct gcaacagcat cagtgcctgc atgtacgctt caggacgcgg cgggcag
//
LOCUS       DAEVZA010000320         1478 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEVZA010000320 DAEVZA010000000
VERSION     DAEVZA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.324"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..538)
                     /locus_tag="LAF92_005219"
     CDS             complement(<1..538)
                     /locus_tag="LAF92_005219"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Has polymerase, DNA-binding and 3'-5' exonuclease
                     activities. In Aeropyrum pernix this protein is sensitive
                     to aphidicolin and stable at 95#C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBI9794088.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVY"
     gene            complement(613..1308)
                     /gene="araD"
                     /locus_tag="LAF92_005220"
     CDS             complement(613..1308)
                     /gene="araD"
                     /locus_tag="LAF92_005220"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBI9794089.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
BASE COUNT          311 a          408 c          415 g          344 t
ORIGIN      
        1 tataaacgat gcgctgcccg cagccttcca ggccgatgca gtacagctca ccgtggcggg
       61 tggtttcaat atctatagaa acccacttga gcggcggacg atagtcggga tgcggtttca
      121 gacgggcatt aacgatagtg ccattgtgca tatcaccctc gacccacacc ggtgaggtga
      181 taaaccgctc catcagatag cgttctggcg ggcgcacatc ggcctcgtag acggtaacgc
      241 caccttcacg caggcgcttt tcgtaattca tcaattggcg atgggcgcga cagtaaaggc
      301 catacaccgg ctggcggtga aaatccttta acgccagcgg tgtcaggcga aagccttgtt
      361 caccctgcaa aatatgctga gcgcggggaa cctgatcggc gggaataaac gccacggact
      421 cttgcggtgc aagcgtaacc tgcaacggcc cgttgtccgt cgccagccag aaggagactt
      481 ctgtcccttg cggggtgtcc cgccagtgtc gggttaagat aaaacctgcc tgcgccacgc
      541 tgaaaatcca tcaaaaaacc aggcttgagt atagcctggt ttcgtttgat tggctgtggt
      601 tttatacagt cattactgcc cgtaatatgc cttcgcgcca tgcttacgca gatagtgttt
      661 atccagcagc gtttgctgca tatccggtaa ctgcggcgct aactgacggc agaatatccc
      721 catataagcg acctcttcca gcacgatggc gttatgcacc gcatcttcgg catttttgcc
      781 ccatgcaaac gggccgtggg aatggaccag aacgccgggc atttgcgctg catcgatacc
      841 ctgtttttca aaggtttcta cgatgacgtt accggtttcc cactcatatt cgccgttgat
      901 ttctgcgtcg gtcattttgc gggtgcaggg aatggtgccg tagaaatagt cggcgtgggt
      961 ggtgccgatt gctggaatcg actgacccga ctgcgcccag atggtggcgt ggcgcgagtg
     1021 cgtatgcaca atgccgccaa tggaggggaa tgcctgatag agcagccggt gagttggcgt
     1081 gtcggaggag ggctttttcg taccttcaac cacttcaccg gtttcgatgc taaccacgac
     1141 catatcgtca gcggtcatga cgctgtaatc gacgccggaa ggtttgatca caaagacgcc
     1201 gcgctcgcga tcaacggcgc tgacgttgcc ccatgtgagc gtgaccaggt tgtgttttgg
     1261 cagcgccagg ttggcttcta atacctggcg tttgagatct tctaacatgt tgactcctga
     1321 gaggtcgttt cccctcaccc cggccctctc cccaaagggg cgagggggaa aacaatgccg
     1381 gatgcgcttt gcttatccgg cctacaaaat cgcagcgtgt aggccagata agacgcgtca
     1441 gcgtcgcatc aggcattgtt ttccccctcg cccctttg
//
LOCUS       DAEVZA010000321         1464 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEVZA010000321 DAEVZA010000000
VERSION     DAEVZA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.325"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(70..474)
                     /locus_tag="LAF92_005221"
     CDS             complement(70..474)
                     /locus_tag="LAF92_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBI9794090.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(695..1426)
                     /gene="bluR"
                     /locus_tag="LAF92_005222"
     CDS             complement(695..1426)
                     /gene="bluR"
                     /locus_tag="LAF92_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HBI9794091.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          379 a          330 c          317 g          438 t
ORIGIN      
        1 tgtactttta ttcccattat gctaatgcct tactgaatta tgaagcattt cttaagcatc
       61 caactttagc tagattaatg gtttattatt ttctacatct tcaatatata aaagcgtatt
      121 atcaatggcg tagtaactgc gtttgttatg attaacatca gtaacccacc ggaaaacgcc
      181 cgcgcctgct agtgttgaac agtattcccg aaatgtagat tttccgcaaa tatgaagcaa
      241 tgcggcctct tttattttag cagggttctt ggtcgtacta acttttaaca ggttcctggt
      301 tcctcttaat aacaaaaccg tgtcatcgtg agtaataatt ctgatgttat ccgtagccag
      361 ataataaatg taatgtgcaa tacggtgatg ttttaattct gaataaaacc aggagaagtt
      421 ttgctctttt ctcacttgct caaacatctt ttgaaaaaca acgacctgat ccatcaggat
      481 aataacctct tgttagttgt gagactgcgt agtgtgcacg atcggtttta ccacttcaat
      541 ctggtctgtc ctttggctgt gatatgtaca gagtgtgata gagggaatat ctgaattctc
      601 ccggtgagca ttttgcaacg gaccagctcc ggtacaaacg ctgttgtggg ttcagattat
      661 aacattctgt ctaaggggcg ggataaaggt gaaattaggg ggcatgaaag atgactttat
      721 aaccttgctc accccaatgt tgtaaaagtt cgttttgcct tctcgttggt gccatgcctg
      781 tccagacaat caatgtttgc gtcgggaaca gttccgggcg cggcgattca atgggatcag
      841 caagaacaga aatgtgccat cctgacaatg ataaacgcca tgcttccagc cacaatcgtg
      901 ctctctcttc aacattccac gccatcagaa tggcttcttt accgggctta cggcgcattt
      961 cgaaaagcga ggttgctgcg tattcaatta atgcgccgtc aaacatactg ctcataatgc
     1021 gggaggtgtt gtgatcaagc acgagacgct ggcgaacagg aaggtaaaca tgattaatca
     1081 attgatctac tgggtactct cgacccagtg aaataattct cgcgcgtagt ttggcaggat
     1141 tagccatgcg aagaattgac atcatctctt cttgcaggcg gctccagtca tcttccgtat
     1201 cctggctggt ggtttccagt aatgctttaa ctttgcctac agggacgcca ttacttatcc
     1261 aacgcttgat ctcttcgatg cgttgtatgt cttcttcatc aaagagtcgg tgtccgcctt
     1321 cactgcgctg tggttttaac aaaccgtagc ggcgttgcca ggcccggaga gtgacaggat
     1381 taatcccgca acgttcagca acatcaccaa tgctgtaata agccacaatt cctccttgcg
     1441 gtcacaaatc tccgtcgcct gtac
//
LOCUS       DAEVZA010000322         1416 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEVZA010000322 DAEVZA010000000
VERSION     DAEVZA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.326"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            141..>1416
                     /gene="tldD"
                     /locus_tag="LAF92_005223"
     CDS             141..>1416
                     /gene="tldD"
                     /locus_tag="LAF92_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBI9794092.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGA"
BASE COUNT          324 a          348 c          426 g          318 t
ORIGIN      
        1 cagccgttgc ctgatgcgac gctgtgcgtc ttatcaggcc tacaaaccgg aacataaccg
       61 taggctggat agggcgttca cgccgcatcc ggcagccgta aaaaatcctc tactgcagta
      121 actaacgagt agcaaaaacg atgagtctta acctggtaag tgaacaattg ctagcggcga
      181 acggcctgaa acatcaggac ttgttcgcga tcctcggtca actggccgaa cgtcgccttg
      241 attatggcga tctctatttt cagtcgagct atcacgaatc ctgggtttta gaagaccgca
      301 ttattaaaga tggttcttac aacatcgatc agggcgttgg tgtgcgtgca atcagcggtg
      361 aaaaaaccgg atttgcttac gctgaccaaa tcagcctgct ggcgctggaa cagagtgcgc
      421 aagcggcgcg caccatcgtc cgtgatagtg gtgatggtaa agtacagacg ctgggcgcgg
      481 tagagcatag cccgttgtat acctcggtag atccgctgca aagcatgagc cgtgaagaga
      541 agctggatat cctgcgtcgc gtcgataagg ttgcccgcga agcggacaag cgcgtacagg
      601 aagtgactgc cagcctcagt ggtgtctatg aattaatttt ggttgcggcc accgacggca
      661 cgctagcggc ggatgtccgt ccgctggtgc gtctttccgt gagcgttctc gtcgaagaag
      721 atggcaaacg cgaacgcggt gccagtggcg gcggcggtcg ttttggttat gaattcttcc
      781 ttgccgatct cgacggcgaa gtccgtgcgg atgcatgggc aaaagaagca gtgcgtatgg
      841 cgctggtcaa tctttctgcc gttgctgcac cagcgggcac catgccggta gtacttggcg
      901 caggttggcc gggcgtgctg ttgcatgaag cggttggtca cggtctggaa ggcgacttca
      961 accgccgtgg cacttcagta tttagtggac aggtcgggga gctggtggct tcagaactgt
     1021 gtaccgtggt tgatgatggc acgatggtcg atcgccgtgg ttcggtggcg attgatgacg
     1081 aaggtacgcc aggccagtac aacgtgctga ttgagaacgg cattctgaaa ggctacatgc
     1141 aggataaact caacgcgcgt ttgatgggga tgacgccgac tggcaacggt cgccgtgaat
     1201 cctacgccca tctgcccatg ccgcgtatga ccaacaccta tatgctgccg ggtaaatcga
     1261 ccccgcagga aattattgaa tccgttgagt acggtatcta tgcaccgaac tttggtggcg
     1321 gtcaggtgga tatcacctcc ggcaaattcg ttttctccac ttcagaagca tatctgattg
     1381 aaaacggtaa agtaacgaag ccggtgaaag gcgcaa
//
LOCUS       DAEVZA010000323         1407 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEVZA010000323 DAEVZA010000000
VERSION     DAEVZA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.327"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..229)
                     /gene="smg"
                     /locus_tag="LAF92_005224"
     CDS             complement(<1..229)
                     /gene="smg"
                     /locus_tag="LAF92_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBI9794093.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPE"
     gene            complement(201..1325)
                     /gene="dprA"
                     /locus_tag="LAF92_005225"
     CDS             complement(201..1325)
                     /gene="dprA"
                     /locus_tag="LAF92_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBI9794094.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
BASE COUNT          354 a          385 c          326 g          342 t
ORIGIN      
        1 cctcaggggt ataaatacgc atggagagtg gatcagaggc cagttgcatc ggttctgcca
       61 gtccttcctg ataatccgca agcttttcca gccaaagcag ggcattgtag atatcttctc
      121 gatcaaatcc tgcgtcggta agatcctgtt caagtttgtc ttgatccaca cgcaactcag
      181 cttctgtgtg aatataggtt tcaaacaaat acattagtac gtcgaacatg gcatgccctc
      241 ctcaatcgga catagccgcc gggtacagct gcgatccatc ctgctaactc cagttcgagt
      301 agttgagtaa ctacctctgg cacaggttgg ccggcacgtt cagcgacgac gtcaacaggt
      361 gttacctcat ctcctacgtt agccaggagc tcaggaaatg gcaatgccac gtcttgctga
      421 tctggtgaat aaaatgaatt ttcaggggcg tctggcaacc agtgcaatcc aaattgcaag
      481 ttttccagaa tttcttccgg ttccgtcaca agaatcgcac cttgttttat taaccagtga
      541 ggcccttcgc ttcccggatt ccctattgga cctggcaagg caaaaacttc acgcccctgc
      601 tcaagcgcac aacgtgctgt caccagcgaa ccactgcgca aagccgcttc caccaccagt
      661 acacctttac ttagaccact gataatgcga tttcttcgtg ggaaattgta agcaaggggt
      721 ggaacatcga ggggaaattc cgagacgaga gctccgccat gttcaagcag actggcagcc
      781 agtcgggcat ggcggcgggg atgaatggta ttaagtccat tccccaatac agcaatgctg
      841 acgccattta cctgtaaggc cgctttatgc gccacaccat cgattccacg cgccagtcca
      901 ctcgtaattg tcactccacg cgtcgccaga gtttcgcaaa ataaccgtcc ccatcgctcg
      961 ccataccatg aatgcgcccg actccccact acggcaagct gaaatgaatg cagcgcgtgc
     1021 agttctcctt caacaaacag tgcgccgggg taatctgtcg tcaccagaag ttgaggagga
     1081 taaaattcgc tgtccgcagg gattaaatga tggttgggtt gctccaacca acaaagtgag
     1141 ctttcgatac tctttcgcgg aaatgaaaga aagcgttgtg cctgccgcaa tgtaagccct
     1201 gtttgctgca atccaaccgc atcaatttgc gactgttttg ccagccagtg agctatacgg
     1261 accatatcat cgccgtacaa gctgctgata ctcattaaac gcagccaaat ttctgtatcg
     1321 accatcctta tctccctgcc ataagcagcc ttagcaatct ttgcgattgg tcagtgatgc
     1381 tgtcaatcag agggggattt gtctaga
//
LOCUS       DAEVZA010000324         1351 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEVZA010000324 DAEVZA010000000
VERSION     DAEVZA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.328"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..409
                     /gene="yeeO"
                     /locus_tag="LAF92_005226"
     CDS             <1..409
                     /gene="yeeO"
                     /locus_tag="LAF92_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBI9794095.1"
                     /translation="TAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALFMPIWSA
                     SWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMFADWAVR
                     AVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            510..585
                     /locus_tag="LAF92_005227"
     tRNA            510..585
                     /locus_tag="LAF92_005227"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:543..545,aa:Asn,seq:gtt)
     gene            complement(1038..>1351)
                     /locus_tag="LAF92_005228"
     CDS             complement(1038..>1351)
                     /locus_tag="LAF92_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI9794096.1"
                     /translation="VIVFKGTDPDHIFEILLEAEVDVRDVTEEEGNIVIYTEPTDLHK
                     GIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGLVDALEDDDDVQKVYHNVAN
                     L"
BASE COUNT          367 a          261 c          306 g          417 t
ORIGIN      
        1 aacggccatc gcctggctaa cggctccctt tgccggggtt atggcatcgt tttacaccca
       61 ggatccacag gttaaacatg tcgttgtgat tctgatttgg ctaaatgctt tatttatgcc
      121 tatttggtcc gcctcatggg tgctacccgc tggatttaaa ggtgctcgtg atgcccgtta
      181 cgccatgtgg gtttcgatgt tgagcatgtg gggttgtcgg gttgtagtcg gttatgtgct
      241 gggaatcatg cttggctggg gtgtggttgg tgtctggatg ggaatgtttg ccgactgggc
      301 tgtgcgggcc gtgctgtttt actggcgaat ggttactgga cgttggctat ggaaataccc
      361 tcgacccgag ccgcaaaagt gtgaaaaaaa gccagttgtg tcggaataaa cgacaaaatg
      421 cagattattt cagcaaacga tttcaaattt aaaaaacagg ctttgacatt gtgggtgggc
      481 atcgctaata ttcgcctcgt tctcacgatt cctctgtagt tcagtcggta gaacggcgga
      541 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattt tagttttgag
      601 acatctctat gagtctcaaa acagttaaaa aataaaaatt atttttaccc ggttaccttg
      661 taaaaaagtg accgggtttt ttgatttctg tgtttcgtgc gcttaatcga gtctcacgct
      721 tcagtgagca aattgtccct ccccaacaga catcacgatt cgcaaggtca aaaactctgt
      781 tccatgaagt accgtacaaa gataaagaga aaaatgcctt atgcgggatg tcgctatcgc
      841 gtctttatcg ggaaactatg cgtaattgcc actttgcctt ctgtaatcag aaaaagaaga
      901 actggaattt taataaatat tatttctctg ggaacggggg ggtataatca atatgacgag
      961 ttgaatatta tattttttat aagaattata tgagattaaa gaaatctgcc gtaaagacag
     1021 atttctttaa aagataatta gagatttgcg acgttatgat aaactttttg tacatcgtcg
     1081 tcatcttcaa gggcatcaac aagcccttca aagatttcta aatcttctgg ggaaagctca
     1141 acttcagatt gagcaatcat ttctaattct gttgttgaga actcagtgat tccagctgct
     1201 tttagagccg cgattccttt atgaaggtca gtaggttcag tataaataac aatgttacct
     1261 tcttcttcag tcacatcacg aacatcaact tcagcttcaa gtaaaatttc aaaaatatgg
     1321 tcagggtctg tccctttaaa tacaatcaca c
//
LOCUS       DAEVZA010000325         1308 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEVZA010000325 DAEVZA010000000
VERSION     DAEVZA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.329"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..333)
                     /locus_tag="LAF92_005229"
     CDS             complement(<1..333)
                     /locus_tag="LAF92_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HBI9794097.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNA"
     gene            complement(308..1108)
                     /gene="otsB"
                     /locus_tag="LAF92_005230"
     CDS             complement(308..1108)
                     /gene="otsB"
                     /locus_tag="LAF92_005230"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBI9794098.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
BASE COUNT          297 a          351 c          318 g          342 t
ORIGIN      
        1 cgcatttacg cgtagatagc cgtcccaggc aggacgctga aattgcacca gatcgagccg
       61 ataatgaaaa gcgggccaga gaacggcatt ggagaattgg ttgtagtatt cgtcaaggtc
      121 ctgttcgctg aggttaaaag aggcccacgt aatgttacct tttttcacct tttttagcgg
      181 ctgatcctca ttccctgttt caccactcca gccaaaccac agtccgcctg cggctttcag
      241 tgcccccagt atgccaacgg caaggccacc ggcactggcg gcgtgctcgt ctggtggtgc
      301 aatccggtta gatactacga ctaaacgact catagtcatc actcctgtta ttttctcttt
      361 tttgttgtaa tgcggtggtt atcatttcaa gccagctcca gacatccggc acacccgcca
      421 gtcgccatga tgcctgagtt gcacctgtgc caatttttac tgacattccg cccagtcggt
      481 taacgactgc gaagccagat tcatcggtta aatcatcgcc cagaaatacg ggcgttcgcc
      541 cgataaaggg agcttcctgc ataaaagctg caattgcctc acctttactg gtacctctcg
      601 gtttgatctc gacaacacac tttccctgct gtaacgccat ttgtggccag atctgagtaa
      661 tacgttgcgc taatgtcatt aatgcgtctt catgctgcgg agcctgacga taatgcagcg
      721 caaaagccat ccctttcgtc tccagctccg caccgggata ctgggcgatg accgtatgca
      781 gttgcacgct aatatcacgc gcaatcgcat ccggcagatg aacgatatgt gttttaccat
      841 tgatgtcacg gcgctccgcc ccatgcacgc ccgctaacgg gaagcgataa ggttttgcca
      901 gtgcgtcaag ctccaccatt gagcgccctg atatcaatgc caatgcacca tcacttgcgg
      961 ttgccagtag ctgtagtcct tgcagaatat tgtcaggcac gacgacctga tcgggatgcg
     1021 gtttgatttc cgccagcgtt ccatcgagat caaaaaacca ggcatatttc gcggatagtt
     1081 caggggtttc ggttaacggt tctgtcaccc ggctctcctc cttctctttt cttattgttt
     1141 tgcgcagaac gcgctttaca gacatgtaag tatagacagt gtgacggggg tcgccatttg
     1201 caatgaagaa cagccaaccg ttgtggcggc tggctgtggt gggaaaaaac gttaaattgt
     1261 tgtggaaaaa agcatcagac agtgcgtttc gctttttgct tgtaacgg
//
LOCUS       DAEVZA010000326         1299 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEVZA010000326 DAEVZA010000000
VERSION     DAEVZA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.330"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..642
                     /locus_tag="LAF92_005231"
     CDS             <1..642
                     /locus_tag="LAF92_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBI9794099.1"
                     /translation="EVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLVPP
                     YPFVQLATLKQRTEKAARTAGRAPAGSQAHRLVPLSDHHYVSQLQMMVATLKIPLERR
                     NKRTGRTEKARIWDITDRTVRTWIGEAVEAAAVDGVTFSVPVTPHTFRHSYAMHMLYA
                     GIPLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFQMPEADAVAMLKGNI"
     gene            complement(700..957)
                     /locus_tag="LAF92_005232"
     CDS             complement(700..957)
                     /locus_tag="LAF92_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794100.1"
                     /translation="MKLETIQIDNTVRSGLNSFTDGVIIRDTKVLITLNLPDGQKTTI
                     EGEWRHMSDANRLSERITMLQHAIYEQYEEYRADIPVSWSR"
     gene            complement(1086..1190)
                     /locus_tag="LAF92_005233"
     CDS             complement(1086..1190)
                     /locus_tag="LAF92_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794101.1"
                     /translation="MRSFTIMSLLETFLTASQGATILFLAMLIYWQKR"
BASE COUNT          305 a          347 c          336 g          311 t
ORIGIN      
        1 gaagtcagcg ccctgctcca ttatgtgccc gatttgcacc gcaagatgct gctggcaacc
       61 ctctggaaca ccggcgcacg tatcaacgag gcgctggcgc tgacccgggg ggatttttcg
      121 ctggtgccac cgtatccgtt cgtgcagctg gccacgctta agcagcgcac cgaaaaagcg
      181 gccaggacag ccggccgagc tcccgccggt agccaggctc atcgtctggt tccgctttcc
      241 gatcaccact acgtcagcca gctgcagatg atggtggcca ccctgaaaat tccgctggaa
      301 agacgcaaca agcgtaccgg cagaacagag aaagcccgca tctgggacat caccgaccgg
      361 acagtacgga cctggattgg cgaagcggtt gaagcggccg ccgtcgacgg ggtgacattc
      421 tcggtgccgg tcaccccgca tacgttccgc cactcctacg cgatgcatat gctgtatgcc
      481 ggcataccac tgaaggtttt gcagagcctg atgggccaca aatcgattag ctcgacggaa
      541 gtctatacga aggtgttcgc acttgatgtt gctgcgcggc atcgagttca gtttcagatg
      601 cccgaagcgg atgcggtagc catgctgaaa gggaatattt aagtacttag gagaatgatg
      661 ctggcgaacg tcctgctcgc cggggaggtc tgtcagacgt tatctggacc agctgacggg
      721 aatatctgct ctgtattctt catactgctc atagatcgca tgctgaagca tcgtaatacg
      781 ttctgacaac ctgttggcgt ccgacatatg ccgccattcc ccctcaatag tggttttctg
      841 tccatccggg agattgagcg tgatcaatac ctttgtatca cgaataatca ccccgtcagt
      901 gaaagaatta agaccactcc ttacggtatt atctatctgt atagtttcaa gtttcatctt
      961 gttacctcct gttgaccgga tcccaagcat agatatctga attcaaattg cctagtgaaa
     1021 tgtctggata tgccgcattt ttccccttat acagcgaagc tggccaaagg ccagcccgct
     1081 tggatttatc gcttttgcca gtaaatcagc atggcaagga acaggattgt tgccccctga
     1141 gaagcggtca ggaaagtttc cagcaatgac attatcgtaa aactccgcat tattaatttc
     1201 aggtgtgtga ttcaggggcc ttagtccccc ttttacagga cgtagtgttc tgtcacgacg
     1261 gaagccaggg ttaataaaaa cgattcgaat atgtctgca
//
LOCUS       DAEVZA010000327         1259 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEVZA010000327 DAEVZA010000000
VERSION     DAEVZA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.331"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            69..371
                     /locus_tag="LAF92_005234"
     CDS             69..371
                     /locus_tag="LAF92_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBI9794102.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            418..840
                     /locus_tag="LAF92_005235"
     CDS             418..840
                     /locus_tag="LAF92_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBI9794103.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            837..1028
                     /locus_tag="LAF92_005236"
     CDS             837..1028
                     /locus_tag="LAF92_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794104.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
BASE COUNT          272 a          309 c          391 g          287 t
ORIGIN      
        1 ttctaccgta tctgtttggc aacgattttc tgtatgccga ggcgtatgtg tacgcgctgg
       61 cgcaaaaaat gatgccggaa tatcagggag gcttctggca cttcatccgc ctgccggacg
      121 gtggcggtta catgatgccg gatggcgacc gtttccacat ggtgaacggt gcaaactggt
      181 ttgaccgtac cgtgagtgct gatgccgcag gcatcattct gacttccctt gtgattaacc
      241 gccagttgtg gctgtaccac gacagcgggg atgcaggact gacccagctt taccggatgc
      301 gcgatgcgca gttgtggcgt cacatcgaat ttcaccccga atgcaacgcg atttacgcgg
      361 cactggactg attaacggac ggggcggcaa tgccgcccct gtaaaggaga cgagaacatg
      421 tactgtactg ttaaagaaat tatccgcgaa gtgctgaaca cagacgtgcc ggacagtgaa
      481 tgcgtttttg ccgtggtgct gacccgtggg gatgtgcgtc acatagccca ggactggagt
      541 ctgacagacg atgagctgga aaccgtcatg cagcgactgg acgatgcctt tgcgcatggt
      601 gcggatgtca gcattgttca cgacgttgtt cgtgagctga tggaagaaaa gcgcgccagc
      661 cgtcaggtga cagttccggc ggtgatgctg gaaaaagtga tggcgctggc aggcagtgaa
      721 atgaagcgcc tgtatgccgt cgggagcgag aacgggggcg acggtgacgc attcgtcagg
      781 gaagaacgcg aagcaatgga cgttgtgtta caggcgctgg acggggagca catgtcatga
      841 atatcagcac agaaacccgc gaaattctgc gcaattacaa agccgtgatt aatgcgcggc
      901 gtcgtgaaat ggggcagaaa ccgctcacca ctgcgcagat tgttgatgaa atctgcgatt
      961 ttgtggcgaa tcagcaggct gtattcctcg gcggtcacta catccttcag ggcagcataa
     1021 acaggtgata caggggcagg cggtggaggc cgcctgtcag cccgcgcgaa aacccctggc
     1081 aggctgacgc ctgccggaag ggaagcgggc cacgctggcg cgtcgcccgt tacccttccc
     1141 gtctgtgatg tgtctgactc aggagtgttt ctttcttatg ttatggaact gttcctgatg
     1201 ttgttgttcc tttatctact gctctgattc tgactactgc agtgttgttc tgtctgccg
//
LOCUS       DAEVZA010000328         1242 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEVZA010000328 DAEVZA010000000
VERSION     DAEVZA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.332"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            174..1154
                     /locus_tag="LAF92_005237"
     CDS             174..1154
                     /locus_tag="LAF92_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HBI9794105.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          305 a          305 c          369 g          263 t
ORIGIN      
        1 cagagaaaat cggccagttt tctctgcctg cagtccgcat gccgtatcgg gccttgggtt
       61 ctaacctgtt gcgtagattt atgcagcgga ctgcctttct cccaaagtga taaaccggac
      121 agtatcatgg accggttttc ccggtaatcc gtatttgcaa ggttggtttc actatggaac
      181 atgaacttca ttatatcggt atcgacaccg ctaaagagaa actggatgtc gatgtgttgc
      241 gtcctgatgg tcgtcatcgc accaaaaaat tcgctaacac cactaaaggg cacgatgagc
      301 tggtgagctg gctgaaaggt cacaagattg accatgcgca tatctgcatc gaagcgaccg
      361 gcacctatat ggaacctgtc gctgagtgcc tttacgatgc tggctacata gtgtcagtca
      421 ttaatcctgc gctgggtaaa gctttcgctc agagtgaagg actgcgtaac aagactgata
      481 ccgtggatgc gcgcatgctg gcagagttct gtcgtcagaa gcgccctgca gcctgggaag
      541 cgcctcaccc gcttgaacgc gcgttgcgtg ccctggtagt ccgccaccag gcgctgacag
      601 atatgcacac gcaggaactg aatcgcactg aaacggcgcg ggaagtccag agaccgagca
      661 ttgatgctca ccttctgtgg cttgaagcag agctgaagcg tcttgagaag cagataaaag
      721 acctgacaga cgatgatccg gatatgaaac accgcaggaa actgctggaa agcatcccgg
      781 gtatcggaga gaaaacatct gcggtattgc tggcttatat cggtctgaag gaccgcttcg
      841 cccatgccag acagttcgcc gcttttgcgg gtctgacacc acggcgttat gaatcaggta
      901 gcagtgtgag aggggcgagc cggatgagta aggccggaca tgtgtcgctt cgcagggcgt
      961 tgtatatgcc cgcaatggta gccaccagta agactgagtg gggacgggcg ttccgcgacc
     1021 gtctggcggc taatggcaag aaaggaaagg tgattctcgg cgcgatgatg cgcaagctgg
     1081 cacaggtggc gtatggagtg ctgaagtcag gcgtgccgtt cgatgcgtca cggcataatc
     1141 cggtagcggc gtaaaaatcg cggaagggat gaaaaaaaca gcgcctgacg gcgctgtgtc
     1201 tggcatgcct gcaatccggg aaaccggacc aggaaaaaac tt
//
LOCUS       DAEVZA010000329         1211 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEVZA010000329 DAEVZA010000000
VERSION     DAEVZA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.333"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>1211
                     /locus_tag="LAF92_005238"
     CDS             <1..>1211
                     /locus_tag="LAF92_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBI9794106.1"
                     /translation="VGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWD
                     GTLKPAYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGT
                     EPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWAYNRSNVV
                     MPDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDA
                     FGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGG
                     RVLAVNSQTRTLTLDREITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPD
                     GVAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQ
                     SGTVNGVTPPA"
BASE COUNT          255 a          321 c          409 g          226 t
ORIGIN      
        1 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
       61 tcttcgcggg cgcattctgc aggtgccgtc gaactataac ccgcagacgc gacaatacag
      121 cggtatttgg gacggaacgt taaaaccagc atacagcaac aacatggcct ggtgtctgtg
      181 ggatatgctg acccacccgc gctacggcat gggaaaacgt cttggtgcgg cggatgtgga
      241 caaatgggcg ctgtatgtca tcggccagta ctgcgaccag tcagtgccgg acggctttgg
      301 cggcacggag ccgcgcatca cctgtaatgc gtacctgacc acacagcgca aggcgtggga
      361 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
      421 gacgttcgtg caggaccgac cgtcggataa ggtgtgggcc tataaccgca gtaatgtggt
      481 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
      541 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
      601 tgaagatacg caggccattg cccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
      661 ctgtaccagc cgggggcagg cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct
      721 ggaaacgcag accgtggact tcagcgtggg tgcggaaggg cttcgccatg taccggggga
      781 tgtcattgaa atctgcgatg atgactatgc gggtatcagc accggcgggc gcgtgctggc
      841 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
      901 taccacgctg ataagcctgg ttgacggtca gggtaatccg gtcagcgtgg aggtccagtc
      961 cgtcaccgac ggcgtgaagg tgaaagtgag ccgtgttcct gacggcgttg cagaatacag
     1021 cgtgtggggg ctgaagctgc cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg
     1081 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtaccgg aaaaagaagc
     1141 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
     1201 gccgccagcg g
//
LOCUS       DAEVZA010000330         1208 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEVZA010000330 DAEVZA010000000
VERSION     DAEVZA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.334"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>1208)
                     /gene="traD"
                     /locus_tag="LAF92_005239"
     CDS             complement(<1..>1208)
                     /gene="traD"
                     /locus_tag="LAF92_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBI9794107.1"
                     /translation="EIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVK
                     SYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTI
                     FAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAV
                     LTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMW
                     LSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQ
                     LEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQYSYGADPVR
                     DGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARPKVAPEFIPR
                     DINPEMENRL"
BASE COUNT          285 a          333 c          312 g          278 t
ORIGIN      
        1 ctcaggcggt tctccatttc cgggttgatg tcacgcggaa taaactcagg ggcaaccttc
       61 ggacgtgcct gatatttcag tgagagtttt actgccggat acggtccggg cagggtgaca
      121 taacaggtca aatccggcag agactgaatg tcggaataac tgaccagcgt ctggcgctcc
      181 atatccttac cggtcgatac cccgtcacgt accggatcag caccgtagga gtactgctcg
      241 ctggctttca ggtgctcttt ctcaccaatc tcaccggcag cgaactctgc aatcttatgg
      301 ctgggagaac ggaaaaaggc acgggtgttc atgacgtcaa acagcgtggc agccgctttc
      361 tcaccgtaga tatcttccag ctgggcatag gactggatac caaacacata acagccaccg
      421 aacttacggg cttccggcag gatctccacc aggtccggca gtttgtgtaa cgtgggtagc
      481 tcatcacaga aaaaccacac acgacggtta cggttttctc ccattgccag cagaccacga
      541 atggcaatgg acagccacat ggagatcacc ggcttcaggg aggcatgggt gtcggcattc
      601 gacgagataa acagccagcc gtttttctga tcttcccgga caccccgcat ccagtcacgg
      661 atggtaaaag actcaccgtt atgctcaatc ccctgcaggt aacggatggc tttcacgtag
      721 ttggtcagca cagcacggat ggaaatcgcc gttttctcaa ttttctcttc caccaggttg
      781 gccgccggtg aattacgcag gaaggtacgc agtttttcga ttttgatgga aagcagtgtg
      841 tccaccagtt tgctgtagct gcggttgggg tcattacgca tcaggtacgc cgcttccgca
      901 aaaatggtac gtcctgaacc ctgccagaac gggtcctctt ttgtgcccat cgggatcagg
      961 gtatttgccg tattatcaaa atccggctgt gtcagacact ccttccataa atcccaggcg
     1021 gcacagcggg catccagcgg attcaggatc ttatcgatgg agggatcata gtaactttta
     1081 acaaattccc ctgaacggtc ataaatcacc accatatcac cgcgctgacg ggcgtagttg
     1141 gccagacgac ggataacttc cgacttaccg gccccgaccg tgccgtgcag acagaagttc
     1201 tggatttc
//
LOCUS       DAEVZA010000331         1194 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEVZA010000331 DAEVZA010000000
VERSION     DAEVZA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.335"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>1194)
                     /gene="rseP"
                     /locus_tag="LAF92_005240"
     CDS             complement(<1..>1194)
                     /gene="rseP"
                     /locus_tag="LAF92_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBI9794108.1"
                     /translation="ERFSIGFGKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVV
                     PELRHHAFNNKSVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSI
                     AAEAQIAPGTELKAVDGIETPDWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDL
                     RHWAFEPDKEDPVSSLGIRPRGPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLT
                     QWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPD
                     EYKVVRQYGPFNAIVEATDKTWQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMT
                     AELGVVYYLPFLALISVNLGIINLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYR
                     IGSIL"
BASE COUNT          303 a          344 c          266 g          281 t
ORIGIN      
        1 gcagaatcga gccaatgcga taacaaaagt cttgaacccg ctcggatacc ggtccgccct
       61 tgatcttttc gatcgcaagg aacagcagat gccccccgtc aagtacgggc aacggaaaca
      121 ggttaattat ccctaagttc acgctaataa gcgcaagaaa cggcaggtaa taaactaccc
      181 cgagttccgc tgtcatccca gcccccttgg cgatagagat cggcccactg aggttgttca
      241 gtttcacatc accggtgatc aattttccca gcatactgac cgtcagcttc atcagctgcc
      301 acgttttgtc cgtggcttcg acgatggcgt tgaacggccc atactggcgt acaactttat
      361 actcatctgg caaaggaatg actttcggct caataccgac aaaaccaatc gctttaccat
      421 tacccggttt actctccggg attaatgtca aagacaaggg acttccctgc ctttcgattt
      481 ctaacgctaa ggatttaccc gggttatccc ggacaagcat cacaaaggtc acccactgcg
      541 ttaagggctg accatcgact ttaacgatcc tgtcgcctgc ttgcaaacct gccttgcttg
      601 ccgccgagtt tggctgcaca ttttccagta caggttcaat ttgcggccca cgaggacgaa
      661 tccccagcga agataccgga tcttctttat caggctcaaa cgcccagtga cgtaaatcga
      721 gctttacatc ccgccgttga tcgctgccaa atggcgctac tgtaatggtg gtgctttcat
      781 cgccaatttt atcgaccaac tgcaaacgca cggcatccca atcaggcgtt tcgataccat
      841 ctacggcttt tagttccgta cctggtgcaa tttgtgcttc cgcagctatc gaattggctg
      901 ctatttcacc aaccaccgga cgtacgccag gcacaccaat aataaaaacc agccagtagg
      961 caaagatagc aaaaatgaag tttgcaaccg gacctgcggc aataatcgcc gctcgttggc
     1021 caacagattt attattgaag gcatggtggc ggagttccgg aacgaccggt tctgcgcgct
     1081 catccagcat tttgacataa ccgcccagcg ggatcagggc gataacatat tcggtgccga
     1141 gcttatcagt tcgccgccag agcgccttac caaaccctat tgagaaacgc tcaa
//
LOCUS       DAEVZA010000332         1191 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEVZA010000332 DAEVZA010000000
VERSION     DAEVZA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.336"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            3..599
                     /locus_tag="LAF92_005241"
     CDS             3..599
                     /locus_tag="LAF92_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI9794109.1"
                     /translation="MIIGYARKSTHLQDVTHQVDELTKAGCEQIYHEQISRGGTKRAK
                     NGAPELENCLKALREGDTLVVWALDRLGGSLSQVITLLDDLKKRGITFIAIKDRIDTS
                     APVIGEIYTHLMAIFSNFERNRNIERTRSGLAAARARGRVGGRKPSLSEDDIKQMKIL
                     LADPEMTVGAVAKRFNVSRMTIYRYTTKGNHSEKINDE"
BASE COUNT          280 a          342 c          340 g          229 t
ORIGIN      
        1 aaatgataat cggctatgcg cgtaaatcga cacatcttca ggatgtaacg catcaggttg
       61 atgaactgac aaaagcgggt tgcgagcaaa tctatcatga acagatctca cgcggcggca
      121 ctaaacgcgc caaaaatggt gccccggaac tggagaactg tctgaaggca ttgcgcgaag
      181 gtgataccct ggttgtttgg gcgcttgatc ggctcggcgg ttcattgagc caggtgatta
      241 cgttgcttga cgacctgaaa aagcgcggaa tcacctttat agccatcaaa gacagaatag
      301 atactagcgc tcctgttatc ggggaaatct atactcatct gatggctata ttctctaact
      361 ttgaacgcaa ccgcaatatt gaacgaacga gatctggact cgcagctgct cgcgcaaggg
      421 gccgtgtagg gggaagaaaa ccgtcacttt cggaagatga tattaagcaa atgaaaattc
      481 tgcttgctga tcctgaaatg acagtaggag ctgttgcgaa gcgcttcaat gtttctcgca
      541 tgactattta tcgctacacg acaaaaggca accattcaga gaaaataaat gatgaataac
      601 attctagcct gatgactccg gttaggttgg gtttcgtcga atgcgatgtg ccgccatatg
      661 cccgtcaggg catggaaggc gaccgcgccg gactccaccg gacaccggcc gcaaatcgcc
      721 ggaaactgcg ggactgaccg gagcaacagg ccaacccccc tccctgctaa gccataaccc
      781 agcccgccgc cacgcagctg ccgcacgtcc tccacggggg tgcgcagtgg gcgccgcgcg
      841 cctgcgcgcg ggtacggcgg cccgcctgcg ggtcgcggcg ccgtactgcg agttagcggc
      901 cgccgcgcgg ccggttacgg gggacaccgc accgtcacgg ccagcgcccc actgagctgc
      961 acaatccaca aataacacaa aggtataatt aaagttatgg aaatccgccc accttgccgg
     1021 gcgccggtgc cgtgtggaaa tccgcccacc ttgccgggcg ccggtgctgt gtagaaatcc
     1081 gcccaccttg ccgggcgccg gtgctgtgtg gaaatccgcc caccttgccg ggcgccggtg
     1141 ccgtgtggaa atccgcccac cttgccgggc gccggtgccg tgtggaaatc c
//
LOCUS       DAEVZA010000333         1181 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEVZA010000333 DAEVZA010000000
VERSION     DAEVZA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.337"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..392)
                     /locus_tag="LAF92_005242"
     CDS             complement(<1..392)
                     /locus_tag="LAF92_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI9794110.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFP"
     gene            complement(621..>1181)
                     /locus_tag="LAF92_005243"
     CDS             complement(621..>1181)
                     /locus_tag="LAF92_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HBI9794111.1"
                     /translation="RYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSS
                     MSMGWNFPLYEQVTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVD
                     SRLGDLRPWAQISYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAA
                     YAALSQAENTTNNSDYLYTMGVSARF"
BASE COUNT          322 a          304 c          279 g          276 t
ORIGIN      
        1 tccgggaacc acttcacgca gttagagagc gtcagcaggt aacccgcgcc atccgccaga
       61 ccgaccagca caccggcgct taaccacagc atcatcaggt tgttggaatg cgcggtcagg
      121 aagaagccta atcccagcaa aatgccggaa gccatggtga cgcgtttaac gccaaaacgt
      181 tcctgcaatt tgcccgcaac agaagacgaa attgccagcc ccagacttaa caagccgaaa
      241 gagaaagcga cctggcttac cggcgcatcc agcttggcag aaagcgcgcc attaaacagg
      301 ctccaggtat aaaccgaccc cagtgcaaac tgggtaatga tagtaccgat gagtgtcagc
      361 cagcgggtac gctgataatt tgaaggtgtc atggcagtat tcctgcagta ataaaaagga
      421 aaaatctctg ccgacaacca taacgaagtg cattttatgc ctggggaaaa acggcatgaa
      481 atgccatcag aacggaatga aatgccttag ttcaggaatg aatgacgcga caattattca
      541 ccaaatttat cattttgtaa gttagcaccc actatcacca caagcattgt tattcaatgg
      601 tttcaattgt gactgttgcg ttaaaatctg gcactaaccc ccatcgtata cagatagtcg
      661 ctattgttag tggtattttc tgcctgagat agcgcggcat aggcagcaat attttgattg
      721 agcaacatat cagcgcctac ggtgacatcc agccagttgc cgttctggtt tgtcgccgtc
      781 atccggctta agcctgattg cgccttccag atattctcgc caaattgctg gttatagctg
      841 atttgcgccc agggtcggag atcgccaagc cgggagtcaa cacgccagcc taagctactc
      901 acgctggcat gccataacgg atcggtcagc gtggtagtac tgtcgccaaa ctcgttatac
      961 atcgaggtgg ttgtgccatc gtaatgtaat gccgcgaccg ggccggttgt aacctgttca
     1021 tataaaggaa aattccagcc catactcata ctgctggtca catccacagg agtttcttcc
     1081 gccagattga cttccagtcc cagcgccctt tggctacttt gaatacgttg cgacaaaata
     1141 tcgttgtaat caggttgatg atcactatcc caggtgtaac g
//
LOCUS       DAEVZA010000334         1177 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEVZA010000334 DAEVZA010000000
VERSION     DAEVZA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.338"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(191..592)
                     /gene="pemK"
                     /locus_tag="LAF92_005244"
     CDS             complement(191..592)
                     /gene="pemK"
                     /locus_tag="LAF92_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HBI9794112.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(525..782)
                     /gene="pemI"
                     /locus_tag="LAF92_005245"
     CDS             complement(525..782)
                     /gene="pemI"
                     /locus_tag="LAF92_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HBI9794113.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(875..1171)
                     /locus_tag="LAF92_005246"
     CDS             complement(875..1171)
                     /locus_tag="LAF92_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBI9794114.1"
                     /translation="MILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGALITS
                     LLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
BASE COUNT          322 a          337 c          262 g          256 t
ORIGIN      
        1 acttcaaaac tgcgcaaaac acgcaaatac gcacaagaaa gtagggctgc gcttggaatt
       61 cggaaaataa accacatcaa aactactttt tagccaaata acgtttggga atcaccagaa
      121 tggtgggaca acagcggttt tagtgcccta aatcgtacgt tttcatccag ttgcccctca
      181 aaccccatgt tcaagtcaga atagtggaca ggcggccaag aacttcgttc atgatagtct
      241 ccggaacccg ttcgagtcgt tttccgcccc gtgctttcat atcaattgtc cggggttgat
      301 cgcaacgtac aacacctgtg gtacgtatgc caacaccatc caacgacacc gcaaagccgg
      361 cagtgcgggc aaaattgcct ccgctggtta cgggcacaac aacaggcagg cgggtcacgc
      421 gattaaaggc cgccggtgtg acaatcagca ccggccgcgt tccctgctgc tcatgacctg
      481 cggtaggatc aagcgagaca agccagattt cccctctttc catgtcagat ttcctcctga
      541 ccagtcgccg gtgcatccag ccattctcgt tcttcagctg atatttcagc attcggatca
      601 cactgtgcca gtagctcagc cagtgaatat tgcgggcgtc tgtacggctc aacaatcagc
      661 cggccattat caatgaccat gccaacttca ttatctgtgc ccagagacag cgcattcagc
      721 agtgccggtg ggacggtcag cataactgag ccgccaaccc tcttcagtcg ggtggtatgc
      781 attcttcacc tccataaaag ttatatttaa atataacatc cactaaaaaa acacaccagg
      841 ctttaacgca caatgtttaa taaaaatata actttcaacc aaacagtaaa cccagcgtgg
      901 ttgcttccat atgcagcaat accggcagca gcaggccacc acttctgatc ctggccactg
      961 atgtaatcaa ccccaccagg aacagttctg ccagtgtcag caggttctga tactggctgt
     1021 gcgcggcgac gaacaacaac gacgttatca gcgcccccag ccacatcgtc cagcagtacc
     1081 gtgaacggaa gacgttcagc ataatccccc ggaacagcgt ttcctcattc aacggggcaa
     1141 ggataaagat ggtcagcaac gtcaggatca cgtcagg
//
LOCUS       DAEVZA010000335         1149 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEVZA010000335 DAEVZA010000000
VERSION     DAEVZA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.339"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..682)
                     /locus_tag="LAF92_005247"
     CDS             complement(<1..682)
                     /locus_tag="LAF92_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBI9794115.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATG"
     gene            complement(1071..>1149)
                     /locus_tag="LAF92_005248"
     CDS             complement(1071..>1149)
                     /locus_tag="LAF92_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBI9794116.1"
                     /translation="AEDLAREASFAQGLRAMIGGILTAS"
BASE COUNT          314 a          295 c          257 g          283 t
ORIGIN      
        1 gtccagttgc aaccacctgt tgatccgcat cacgcagttc aacgctgaca tcaccattgg
       61 ccaccacctg ccagtcaaca gacgcgtggt tacagtcttg cgcgacatgc gtcaccacgg
      121 tgatatcgtc cacccaggtg ttcggcgtgg tgtagagcat tacgctgcga tggatcccgg
      181 catagttaaa gaaatcatgg aagtaagact gctttttctt gccgttttcg tcggtaatca
      241 ccattcccgg cgggatagtc tgccagttca gttcgttgtt cacacaaacg gtgatacgta
      301 cacttttccc ggcaataaca tacggcgtga catcggcttc aaatggcgta tagccgccct
      361 gatgctccat cacttcctga ttattgaccc acactttgcc gtaatgagtg accgcatcga
      421 aacgcagcac gatacgctgg cctgcccaac ctttcggtat aaagacttcg cgctgatacc
      481 agacgttgcc cgcataatta cgaatatctg catcggcgaa ctgatcgtta aaactgcctg
      541 gcacagcaat tgcccggctt tcttgtaacg cgctttccca ccaacgctga tcaattccac
      601 agttttcgcg atccagactg aatgcccaca ggccgtcgag ttttttgatt tcacgggttg
      661 gggtttctac aggacgtaac ataagggact cctcattaag ataaaaatac tggtcaacct
      721 ttaatctgat tagttaacca attctgtgtt gcacacagtt atctgtgaga aacccatgga
      781 agagaaatgg aaataaaaag aactgacacc gcgatcaaaa aaccaaaaac tgcaataccc
      841 acagcgtgat attgatcgca ttaatcttta aaacggcgtt gacagaagcc ataaaataag
      901 caaccgaacg ttcgttcgag tatattggga acgataaact ctcatgacat atggctacgg
      961 tgaatatttt tgggggggaa ttctccgatc ccggctagat tgtattttgt acagggaaat
     1021 taaagtatga aacgcgggca cagttatcac cagcccgcga aatgagagga tcaggatgcg
     1081 gttaagatac cgccaatcat cgcgcgtaat ccctgagcaa acgacgcttc acgcgccaga
     1141 tcttccgct
//
LOCUS       DAEVZA010000336         1131 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEVZA010000336 DAEVZA010000000
VERSION     DAEVZA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.341"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>1131
                     /gene="rhsB"
                     /locus_tag="LAF92_005249"
     CDS             <1..>1131
                     /gene="rhsB"
                     /locus_tag="LAF92_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HBI9794117.1"
                     /translation="DRLTHRTVNGETAEQWQYDERGWLTDISHISEGHRVTVHYGYDE
                     KGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAG
                     MKLGDTPLVEYTRDRLHRETLRRFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDN
                     GELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDST
                     LSMWPDNRIARDAHYLYRYDRYGRLTEKTDLIPEGGIRTDDERTHRYHYDSQHRLVHY
                     TRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTT
                     IQNDRSRIQTIYQPGSFTPLIRVETATGELARTQRRSLADAL"
BASE COUNT          264 a          367 c          337 g          163 t
ORIGIN      
        1 agaccgcctc acgcaccgca ccgtgaatgg cgaaaccgca gagcagtggc agtatgacga
       61 acgcggctgg ctgacagaca tcagccatat cagcgaaggg caccgggtga cggtgcatta
      121 cgggtatgat gagaaaggcc ggctgaccgg tgagcgtcag acggtgcatc acccgcagac
      181 ggaagcactg ctctggcagc atgagaccag acatgcgtac aacgcgcagg ggctggcgaa
      241 ccgctgtata ccggacagcc tgcccgccgt ggaatggctg acctacggca gcggttacct
      301 ggcaggcatg aaactcggcg acacaccgct ggtggagtac acccgcgacc gcctgcaccg
      361 ggaaacgctg cgccgcttcg gccgttatga actcaccacc gcttataccc ctgccgggca
      421 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
      481 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      541 cggcaggctg accggcgttc acaccaccgc agcgaacctt gatatccgca tcccgtatgc
      601 cacagacccg gcaggtaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      661 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccgtta
      721 cggcaggctg acggagaaaa ccgacctcat cccggaaggg ggtatccgta cggatgatga
      781 gcgcacccac cggtaccatt acgacagtca gcaccggctg gtgcactaca cgcggacaca
      841 atatgcagag ccgctggtcg aaagtcgcta tctttacgac ccgctgggcc gcagggtggc
      901 aaaacgggta tggcgacgtg aacgggacct gacgggctgg atgtcgctgt cacggaaacc
      961 gcaagtgacc tggtacggct gggacggcga ccggctgacc acgatacaga acgacaggag
     1021 ccgcatccag acgatttatc agccggggag cttcacacca ctcatcagag ttgaaactgc
     1081 caccggtgaa ctggccagaa cgcagcgccg cagcctggcg gatgcgcttc a
//
LOCUS       DAEVZA010000337         1128 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEVZA010000337 DAEVZA010000000
VERSION     DAEVZA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.342"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>1128
                     /locus_tag="LAF92_005250"
     CDS             <1..>1128
                     /locus_tag="LAF92_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="cleaves origin of transfer and unwinds; oriT
                     relaxase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBI9794118.1"
                     /translation="VREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLR
                     DMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDVQGETQVVRISSLDSSWSLF
                     RPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRAEPASLPVSDS
                     PFTALKLENGWVETPGHSVSDSAKVFASVTQMAMDKATLNGLARSGRDVRLYSSLDET
                     RTAEKLARHPSFTVVSEQIKARAGETSLETAISHQKSALNTPAQQAIHLALPVVESKN
                     LAFSHVDLLTEAKSFAAEGTSFADLGREIDAQIKRGDLLHVDVAKGYGTDLLVSRASY
                     EAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATR"
BASE COUNT          250 a          303 c          363 g          212 t
ORIGIN      
        1 gggtacggga acaggccata ctgacacagg ccattcgcag tgagctgaaa acacagggcg
       61 tgctcggaca cccggaggta accatgaccg ccctgtcacc ggtctggctg gacagccgga
      121 gccgttatct gcgggatatg taccgcccgg gaatggtgat ggagcagtgg aacccggaga
      181 cacgcagtca tgaccgctat gttatcgacc gggtgacggc gcagagtcac agcctgaccc
      241 tgcgggatgt gcagggcgaa acgcaggtgg tgcgtatttc ctccctggac agcagctggt
      301 cgctgttccg gccggaaaaa atgccggtgg cagacggcga gcgactgagg gtgaccggga
      361 aaattcccgg actccgagtc tccggcggtg accgcctgca ggtggcatcc gtcagtgaag
      421 atgtgatgac ggttgttgtg ccggggcggg ctgaaccggc ctccctgcct gtgagcgatt
      481 caccgttcac ggcactgaag ctggagaatg gctgggtgga aacgcccggg cattccgtca
      541 gcgacagtgc gaaggttttt gcctccgtca cacagatggc aatggacaag gccaccctga
      601 acggtctggc ccgcagcggt cgtgatgtcc ggctgtattc ctcactggat gaaacccgta
      661 ctgcggaaaa acttgcccgc catccgtcct ttacggtggt ttctgagcag ataaaggcgc
      721 gggccggtga gacatcgctg gaaacggcta tcagtcatca gaagtccgca cttaacacgc
      781 cggcacagca ggccattcat cttgcccttc cggtggtgga aagtaaaaac ctggccttca
      841 gccatgtgga cctgctgaca gaggcaaagt cgtttgctgc agaaggaacc agttttgctg
      901 acctggggcg ggaaatcgat gcgcagataa aacggggcga tttactgcat gtggatgtgg
      961 caaaaggtta tggcacagac ctgctggttt cccgtgcgtc gtatgaggct gaaaaaagca
     1021 ttcttcgcca tattctcgaa ggtaaggagg cggtcacgcc gctgatggag agagtacctg
     1081 gcgaactcat ggagaaactg acatcaggac agcgcgccgc cacccgca
//
LOCUS       DAEVZA010000338         1127 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEVZA010000338 DAEVZA010000000
VERSION     DAEVZA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.343"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(45..782)
                     /locus_tag="LAF92_005251"
     CDS             complement(45..782)
                     /locus_tag="LAF92_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794119.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQNNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNSLKPEAVSC
                     NLLSLCVLAPVFFTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFVQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHTGYTWSVSVN"
     gene            complement(985..>1127)
                     /locus_tag="LAF92_005252"
     CDS             complement(985..>1127)
                     /locus_tag="LAF92_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794120.1"
                     /translation="QTSTETGNQHKYQTRVVSNANKVNLKFEEAKPVLEDQLAKSIAN
                     IL"
BASE COUNT          305 a          267 c          262 g          293 t
ORIGIN      
        1 actccgggaa aatctggaac agggcagaaa aggatgatgt ggggttaatt cacagaaaca
       61 gaccaggtgt accctgtatg ccggtggtga tacccgcctg cagccctgcc ggaagagacc
      121 ttctctgtaa caatgtcagt caggcgcaat acttcagacc agtcactccc atgcggaaac
      181 tggatctgaa caaaaaggcg tacccgctca cggtgatcca gaactgtgtc cggttcataa
      241 aaacggccac tgtcaggaga aacacgggac gttttattga tgagatcata cttatcggca
      301 ggagactgcg atgaaatcgc ccaggtgata ttctccgcct ggtggccatc aatacgactg
      361 ctccccggcg tccatgcaat ttcctgcgca ctcgccacga tgatctccct ggaaacttca
      421 gccagctttc tgggtgtaaa gaataccggt gccagcacac acagggacaa caaattacag
      481 ctgaccgctt ccggtttaag cgaattgctc ccggattttt caggaaaaga tatttttgtc
      541 agtatttcag ttaattcctt atgcctgaga gaattaataa agtacatgat aagcatcatg
      601 agaaacaata taacaataag ccctccgata accactaaaa ggtcagtatc aatattattg
      661 ctgtacagat agtctttagt ggtcactgta ttattctgta tcaccggggg gtattcagtt
      721 acaggcttct cagtaaattg attattctca ccaacatatt cagcgatatt gtctgatgtc
      781 ataatgactt tctccggaag atcccggctt cgccggcaac acttcacggt cgcacaaaga
      841 atttgcacaa ccgctcagtg ttttcatatt tatttcggaa acgtcgggaa gtgacaaaat
      901 ttattttgtt acgtcccggg gttggacaac gagcaacgcg aggcgtcagc aaggaggccg
      961 gtcagaccag cctcctgacg aggttcagag aatatttgcg attgatttgg ccagctggtc
     1021 ttcgagaaca ggcttcgcct cttcaaattt caggttaacc ttgttcgcat ttgagaccac
     1081 acgggtctgg tatttatgct ggttacctgt ttcagtactg gtctgaa
//
LOCUS       DAEVZA010000339         1114 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEVZA010000339 DAEVZA010000000
VERSION     DAEVZA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.345"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>1114
                     /locus_tag="LAF92_005253"
     CDS             <1..>1114
                     /locus_tag="LAF92_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HBI9794121.1"
                     /translation="SRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYAN
                     QIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDAS
                     LKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDAADQRAETRTQAPGA
                     VSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAGIDEAIS
                     REQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYSDAVS
                     VLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERL
                     SGELITGRRQLPEGMAFTPGSTVIVDQGEKLSLKETL"
BASE COUNT          260 a          274 c          381 g          199 t
ORIGIN      
        1 agtcgcgatc aggaaccaca gttacacacg catgcggtgg tggctaatgt cacgcagcat
       61 aacggtgagt ggaagacact gagcagtgac aaagtgggga aaacggggtt cattgagaat
      121 gtgtacgcta atcagattgc ctttggcagg ctctatcggg aaaaactgaa agagcaggtt
      181 gaggcgctgg gctatgaaac cgaagtggtg ggtaagcacg gtatgtggga gatgccgggc
      241 gtaccggtgg aggccttttc cggacgctca caggctatcc gagaggccgt cggggaagat
      301 gcctcgctaa aatcccggga tgtggcggca ctggatacgc gaaaatccaa acagcacgtc
      361 gatcctgaag tcagaatggc tgaatggatg cagacgctga aggaaaccgg gttcgacatc
      421 cgggcatatc gtgacgcggc ggatcagcgt gcggagaccc gcacgcaggc gcccggggct
      481 gtttcacagg aggggccgga tgtgcagcag gcggtgacac aggcgattgc cggattaagt
      541 gaacgaaaag tgcagtttac gtacacggac gtgctggcca gaacagtcgg catactgccg
      601 ccggaaaatg gtgtgattga acgggcacgc gccggtatcg atgaggccat cagccgtgag
      661 caactcatcc cccttgaccg tgagaagggg ctgttcacgt ccgggattca tgtgctcgat
      721 gaactgtcag tccgggcact cagtcgcgat atcatgaaac agaaccgggt gaccgtacat
      781 ccggagaaaa gtgtcccccg gacggccggc tacagtgatg ccgtcagtgt tctggcacag
      841 gaccgtccgt cgctggccat tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg
      901 gtggctgaac tggtcatgat ggcccgggag caggggcggg aggtgcagat tatcgctgct
      961 gaccgtcgct cgcagatgaa cctgaagcag gatgaacggc tgtccggtga gctgataacc
     1021 ggacgtcgtc agctgccgga aggcatggcc ttcacgccgg gcagtactgt tatcgttgac
     1081 cagggcgaaa aactctccct gaaagagacg ttaa
//
LOCUS       DAEVZA010000340         1083 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEVZA010000340 DAEVZA010000000
VERSION     DAEVZA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.346"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>1083
                     /locus_tag="LAF92_005254"
     CDS             <1..>1083
                     /locus_tag="LAF92_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBI9794122.1"
                     /translation="LVKNRCKNGDHYWVSAYVTPIAKNGSIVEYQSVRTKPEPEQVLA
                     AEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSAMAAGMLTDTSISSLLLATLM
                     SGSLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGRTDEFGQIEFALRMMQAETG
                     AIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQIATAVNQMVASIQEVASN
                     AQHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQATQVIHELEGQSNEISKVLD
                     VIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAARTQQSTTDIQSMISAL
                     QERAQSAVTVMEQSSRQAHTSVAHAE"
BASE COUNT          312 a          237 c          285 g          249 t
ORIGIN      
        1 gctagtaaaa aatcgctgta aaaatggcga ccactattgg gtaagtgctt atgtaacgcc
       61 aatagctaag aatggttcga ttgttgaata ccagtctgta aggaccaagc ctgaacctga
      121 gcaggttttg gctgcggaaa aattatatgc tcaattgaga agcgggaagg ccgcgaggcc
      181 gaaattggct gctagctttt ccgtgaaaat actcttgctc atatggggta gtattatatc
      241 aagcgcaatg gctgccggca tgcttactga tacatcaata agcagcttat tgttagccac
      301 tttaatgtca ggaagcttaa gctctgttag tgttttggct attctctctc ctcttggaag
      361 actggttgaa agagccagga atatttccaa taacccatta agtcaatccc tctacactgg
      421 gcgcaccgat gagtttggcc aaatagagtt tgctttacga atgatgcaag ctgaaacagg
      481 cgccatagta ggtcgcatag gtgatgcatc aaatcggctt agcgaacaca cccgaggcct
      541 actaaaggat attgagtcaa gcaatgtact tacagttgag cagcaggcag agacagatca
      601 aatagcaacg gcagtaaacc aaatggtggc aagcattcaa gaggttgcga gcaatgcaca
      661 gcatgctgca gatgcggccg gaagagcaga cactgagacg gcatctggcc agcgtctggt
      721 agcccacaca agccagtcaa tcactgccct tgaaggtgaa attaggcaag ccactcaggt
      781 tattcatgag cttgaaggtc aaagtaacga gatatcaaaa gttcttgacg ttatacgagg
      841 gatcgccgag caaacgaatt tgttggcact caacgcagca attgaggccg cgcgtgctgg
      901 tgagcagggg cgtggttttg ctgttgtcgc cgatgaggtt cgcagtcttg ctgctcgcac
      961 acagcaatcg acaacggata ttcaaagcat gatcagcgct ctacaagagc gagcgcaatc
     1021 cgctgttaca gtcatggagc aaagtagtcg gcaagcgcac acgagtgtag ctcacgcaga
     1081 gga
//
LOCUS       DAEVZA010000341         1044 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEVZA010000341 DAEVZA010000000
VERSION     DAEVZA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.347"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(56..688)
                     /gene="traW"
                     /locus_tag="LAF92_005255"
     CDS             complement(56..688)
                     /gene="traW"
                     /locus_tag="LAF92_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HBI9794123.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(685..1008)
                     /gene="trbI"
                     /locus_tag="LAF92_005256"
     CDS             complement(685..1008)
                     /gene="trbI"
                     /locus_tag="LAF92_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBI9794124.1"
                     /translation="MPGYLAMVLLNAAISYGIVRLNAPVTAAFNMKQTVDAFFDSASQ
                     KQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVSPAVVQGAPDITREIQQDIARR
                     MRAEP"
BASE COUNT          207 a          337 c          279 g          221 t
ORIGIN      
        1 gctgcaggga catgaccggc gagaaggaat aacatcagca gccacaggct tcgcttcatt
       61 ttctgccctc ctctgccgga ataaattcca ccttcaggaa gcgatcgccg ggaacggcgc
      121 tcacccgtgc cgggacctga tcaatgccga ggcgctggca gagcacaccg ttctggtcaa
      181 agtaaacacg gctgtccaga gacttctgca tctccgggat actgccctgc acgaggataa
      241 ttttgctctc cagtgtgggc ggcgtctggc gtttcatcca ggccacctgc gccggatcat
      301 cgccattgat gaaatacagc gtctggttaa aaggcacata ctgcagcgga ttcatcactt
      361 caccctgacg ggcaaacacc cgcccttcgt tatcccggat atccgccgcc agtctgacgg
      421 acggatcaaa caaccggctg ctgtatttct ccgtgcgtcc gataccgggc acggcagggg
      481 gccgcagact gttgcggatc acccgctcct taaaggcatc cattttccgg cccatctcac
      541 cggactgctc cagtgccgtc aggcgctgca tgatcaccgt cagcatgtcc ggctccttta
      601 ccggccacag atcgccccag gtgccaagat cggcggcggc cacactctgc ccccatacca
      661 gcagggcaat cagcccccgg catctcatgg ttccgccctc attcgccggg caatatcctg
      721 ctggatttcc cgggtgatat ccggagcccc ctgtaccacg gcaggcgaca ccagaatgac
      781 cgcgtggtgt ttctgctgcc atgcctgcag gctggcttcc agtgccgtgt taaaacgcgc
      841 tgaaagggct tttgactgtg cttctgacag ttgtttctga ctggcgctgt caaaaaacgc
      901 atccacggtc tgtttcatgt tgaaggcggc agtcaccggt gcgttcagtc tgacaatgcc
      961 gtaactgatg gctgcgttca gtaacaccat tgccaggtat cccggcaccg tccaccacca
     1021 gtggctgcgt cgacgggctg taac
//
LOCUS       DAEVZA010000342         1040 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEVZA010000342 DAEVZA010000000
VERSION     DAEVZA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.348"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            164..421
                     /locus_tag="LAF92_005257"
     CDS             164..421
                     /locus_tag="LAF92_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794125.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            421..1011
                     /locus_tag="LAF92_005258"
     CDS             421..1011
                     /locus_tag="LAF92_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794126.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRLEGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
BASE COUNT          251 a          269 c          309 g          211 t
ORIGIN      
        1 cgcaaatgat atgataatta agaaatatat ataaaatcat atcagatatg gttttcatca
       61 ttgatgataa aacaatatct gatattatca gaaatcatga gcacacaaat catgcaggat
      121 attattttta cctgtcagta cattatcaga tggagcgtgc aggatggatg aagaaagggt
      181 gttttcactc agctatgaac aactgacccg gtttgcggaa aaacgcatcc gggaatgcaa
      241 tctggacagc cagggcgcga tttatctctg cgagtcagcc aaagccggag ccgtgctgat
      301 cttctggcat gaactggcca taaatggcta tgcctcaatg aatgccataa agagacagga
      361 gctcattgac gccgactttc agcgcctcag aaacctcatc tggccggagg atgacaggta
      421 atgacccaga gccgccgacc gtcgccactg cagcggagag tgctgattgt gctggctgcg
      481 cttgatgaaa aacggccggg ccccgtgctg acgcgggacc tggaacgggt gctggagcga
      541 agcggggaag ccccggtcta cgggccaaac ctgcgcgcct cctgccgtcg tctggaggat
      601 gccggctggc tgcgtaccct gcgcgccccg aatctgcagc tggctgtcga gctgacggac
      661 gccggccggg ccgtggcaca gcctctgctg ctggcagaac aggaccgcct ccgggcagaa
      721 cagcgtgcgg cagaagtcgt cgtactgccg ctggtcccgg cagcaggtct gcccgcagac
      781 ggcacttctg ccactgatct ggcggtgcag ctgaacggca taacgtatca ggcctgccgt
      841 ggggattttg tggtacgtct cgacggcagc acctgtctgc agttatggaa taaagaaggc
      901 agggtggtac gcctggaggg cgatccgctg gaagtggcgc agtggctgca ggcctgtcac
      961 gatgccggaa tggaggttcg tgtacaggtt aatgaaagcg tcaccccgta aaaacatacc
     1021 agcacggttt acacatcatc
//
LOCUS       DAEVZA010000343          997 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEVZA010000343 DAEVZA010000000
VERSION     DAEVZA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.349"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>997
                     /locus_tag="LAF92_005259"
     CDS             <1..>997
                     /locus_tag="LAF92_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBI9794127.1"
                     /translation="QAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQD
                     LAVIPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVRE
                     VFTAATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGL
                     FFISVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLS
                     QGGEFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEE
                     EDEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYK"
BASE COUNT          174 a          216 c          325 g          282 t
ORIGIN      
        1 caggcggcgg ttgtcggtgg cattggcctt gcgatgtctt caactgcaat ggcgttgcaa
       61 ttgatgcgtg agaaagggat gaatcgcagc gaatccgggc agctcgggtt ttcggttctg
      121 ctgtttcagg atctggcggt gatcccggca ctggcgttag tgccgttgtt ggcgggttcg
      181 gcagacgaac atttcgactg gatgaagatc ggcatgaagg tgctggcgtt tgtcggcatg
      241 ctgattggcg ggcgctattt actgcgtccg gtattccgtt ttattgcagc ttctggcgtg
      301 cgggaagtgt tcaccgccgc gacgctgctg ttggtgttgg gttccgcatt gtttatggac
      361 gcgctggggc tgtcgatggc gctcggtacg tttattgcgg gcgtgctgct ggcggaaagt
      421 gaatatcgcc atgaactgga aacggctatc gatcccttca aaggcttgct gctcggtttg
      481 ttctttatct ctgtcggcat gtcgctcaat ctcggggtgc tttataccca tctgttgtgg
      541 gtagtgataa gcgtggttgt gctggtggcg gtgaaaattc tcgtgttgta tctgctggcg
      601 cgattgtatg gcgtgcgtag ctcagagcgg atgcagtttg ctggcgtgtt gagtcagggc
      661 ggtgagtttg cctttgtcct cttttctacc gcttcttcac aacgtttatt ccagggcgac
      721 cagatggcgc tgttgctggt gacggtgacg ctttccatga tgaccacgcc gctgctgatg
      781 aagctggtgg ataaatggct atcccgccag tttaacggac cggaagaaga agacgaaaaa
      841 ccgtgggtca acgatgataa accccaggtc attgtcgtgg gcttcgggcg ttttggtcag
      901 gtgattggtc gtttgctgat ggcaaataaa atgcgcatta ccgtgctgga gcgggatatc
      961 agcgccgtta acctgatgcg taaatatggc tacaaag
//
LOCUS       DAEVZA010000344          946 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEVZA010000344 DAEVZA010000000
VERSION     DAEVZA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.352"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..178
                     /gene="rrrD"
                     /locus_tag="LAF92_005260"
     CDS             <1..178
                     /gene="rrrD"
                     /locus_tag="LAF92_005260"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBI9794128.1"
                     /translation="NVGAGNFRTSMLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMT
                     RREIEREICLWGQQ"
     gene            395..577
                     /gene="rzoD"
                     /locus_tag="LAF92_005261"
     CDS             395..577
                     /gene="rzoD"
                     /locus_tag="LAF92_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HBI9794129.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(668..>946)
                     /gene="iss"
                     /locus_tag="LAF92_005262"
     CDS             complement(668..>946)
                     /gene="iss"
                     /locus_tag="LAF92_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HBI9794130.1"
                     /translation="FSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVSGIGQE
                     KTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
BASE COUNT          260 a          220 c          236 g          230 t
ORIGIN      
        1 caacgtgggt gctggcaatt tcagaacatc gatgcttctt cgcaaaataa accagggcga
       61 tatcaaaggc gcatgtgatc agctacgtcg ctggacatat gctggcggta agcaatggaa
      121 aggtctcatg actcgtcgtg agattgagcg tgaaatctgt ttgtggggtc agcaatgaac
      181 agagtaaccg cgattatctc cgctctggtt atctgcatca tcgtctgcct gtcatgggct
      241 gttaatcatt accgtgataa cgccattacc tacaaagccc agcgcgacaa aaatgccaga
      301 gaactgaagc tggcgaacgc ggcaattact gacatgcaga tgcgccagcg tgatgttgct
      361 gcactggatg aaaaatacac gaaggagtta gctaatgcga aagctgaaaa tgatgctctg
      421 cgtgatgatg ttgccgctgg tcgtcgtcgg ttgcacatca aagcagtctg tcagtcagtg
      481 cgtgaagcca ccaccgcctc cggcgtggat aatgcagcct ccccccgact ggcagacacc
      541 gctgaacggg attatttcac cctcagagag aggctgatca ctatgcaaaa acaactggaa
      601 ggaacccaga agtatattaa tgagcagtgc agatagagct gcccatatcg atgggcaact
      661 catgcaatta ttgtgagcaa tacacacgcg cttccagcgg agtataaatg cctaaagtaa
      721 taaaaccgag caatccattt acgaatgttt gctgggtttc tgttttaaca acattttctg
      781 cgccgccaca aattttggct gcatcaacag ttttctcctg tccaattccc gaaacgaaga
      841 agtgatgggt gatggtttcc tttggtgtta ctgctgtcgg tttgtttcca acagtaaacg
      901 tctgttgagc acatcctgta ataagcattg ccagagcggc agaaaa
//
LOCUS       DAEVZA010000345          942 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEVZA010000345 DAEVZA010000000
VERSION     DAEVZA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.353"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..502)
                     /locus_tag="LAF92_005263"
     CDS             complement(<1..502)
                     /locus_tag="LAF92_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBI9794131.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQV"
     gene            complement(502..864)
                     /gene="trbJ"
                     /locus_tag="LAF92_005264"
     CDS             complement(502..864)
                     /gene="trbJ"
                     /locus_tag="LAF92_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HBI9794132.1"
                     /translation="MLPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGRKYRD
                     IPAEMSYAPGEQIKGGQE"
     gene            complement(794..>942)
                     /locus_tag="LAF92_005265"
     CDS             complement(794..>942)
                     /locus_tag="LAF92_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBI9794133.1"
                     /translation="PVATPTTFLVNVNTLEALPLLQGATDAAGFMARMDTVLQMYGGK
                     KGAK"
BASE COUNT          241 a          257 c          234 g          210 t
ORIGIN      
        1 acacctgcgt ccgggggaaa agcccgccga taatcccctg tgccgcctgg caggaactga
       61 ggttcatact gttaatgtcg cttgccattt tctgcaggaa gtcttttgcg gttttgattt
      121 ccggcaccgt tgtctgcagg gcaaggtcaa aaaagtaacc ggcagcattg ctcataatct
      181 gcttaacaaa acgctgcagc tgctcgccat taataaaact gaacgagccg aggtaggcgt
      241 cgatgccgcc acatccggca ttgatatccg gcagcgtcat ggaaatcagc tgaacatttt
      301 tgacctgagt acgggcatac agggagccac cgtaagcata accggcggcc tgcccctgcc
      361 agacgcccgg ctgtgtggta ttggaggcga agcccagctt gttaaagaac tgattcatgt
      421 cgctgttcac atccgctgac gctgcgggtg tgaccgtcag taaagcagca caaagaacca
      481 gaagaggttt aatgcgtggc atcattcttg ccctccctta atttgttctc ccggcgcata
      541 cgacatttca gcaggaatat cccgatattt ccgtccagtc ctgcggctgt aatatatttc
      601 agacaccgaa atccatccca caattgcccc cacaaaaaac acaacagaaa cagaaaaggt
      661 aaggcacagc gaaacgacaa tttctacagg gctggcggga taaaaaagcc cctgatggat
      721 aaaggggata ttcaggctga cgattattcc cgtcgcgatg ccggatattc cggcaatgag
      781 taacaccacc tgcttatttc gcaccttttt tccctccgta catctgcaga acggtatcca
      841 tccgcgccat aaaaccggca gcatccgttg ccccctgtaa aagcggtaat gcctcaagcg
      901 tgttgacgtt gaccaggaag gtggtcggtg tggccaccgg ga
//
LOCUS       DAEVZA010000346          936 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEVZA010000346 DAEVZA010000000
VERSION     DAEVZA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.354"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(1..325)
                     /locus_tag="LAF92_005266"
                     /pseudo
     CDS             complement(1..325)
                     /locus_tag="LAF92_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(356..682)
                     /locus_tag="LAF92_005267"
     CDS             complement(356..682)
                     /locus_tag="LAF92_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794134.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(682..>936)
                     /locus_tag="LAF92_005268"
     CDS             complement(682..>936)
                     /locus_tag="LAF92_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794135.1"
                     /translation="DTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPI
                     NLGLFKNCQDWSNDSLETCKMKCSGNNIGRFIRFVFTGVM"
BASE COUNT          274 a          215 c          190 g          257 t
ORIGIN      
        1 ccaccctgcg gcccagcggg tcgtaaagat agcggctttc gaccagcggc tctgcatatt
       61 gtgtccgcgt gtagtgcacc agccggtgct gactgtcgta atggtaccgg tgggtgcgct
      121 catcatccgt gcggataacc ccttccggga tgaggtcggt tttctccgtc agcctgccgt
      181 gacggtcata ccggtaaaga tagtgcgcgt cacgggcgat acggttatcc ggccacatgc
      241 tgagggcgct gtccgggtgc agctccgggt cgggcaggcg gttacctgcc gggtctgtgg
      301 catacgggat atcaatgccc aggttacata taataataag agcaattaac ttaatttatt
      361 tgtttataat gcgcttactt aatacaaacc attgaatata ccctcctgac agaaaataaa
      421 gccatattat tatcgccata atatacttac tatcaatcct tacgccaata atgtctgata
      481 cagtccctat aaatattaga gcaataaaga atactatgcc tgttggaaaa catattacca
      541 tcattccata agacatgaga ctaattaact cgctcacatc acgagtggag tctaatagtg
      601 aaagtctatg catggaaaag aatagtatta aaagcgataa agccagccat atagctctga
      661 ataaaaaaat aatatatttc attacatcac tccggtgaat acaaatctaa taaaacgtcc
      721 aatgttattt ccggagcact tcatcttaca tgtttctaaa gaatcattag accagtcttg
      781 gcagttttta aatagcccta aatttatagg tgtgaatata tcaggcaata cagaataacg
      841 cggccttacc tctttaaact tcccttttaa acagttctcc acacaactat tatcggtttc
      901 aacgaggtca caacggccag cagcttcttt tgtatc
//
LOCUS       DAEVZA010000347          904 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEVZA010000347 DAEVZA010000000
VERSION     DAEVZA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.357"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..186
                     /locus_tag="LAF92_005269"
     CDS             <1..186
                     /locus_tag="LAF92_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794136.1"
                     /translation="VAEVANAMCDLRTTMNEMEQRYSFNADTLPERLVRQTLFRANRL
                     LMEAYTEILELDACFKD"
     gene            200..634
                     /locus_tag="LAF92_005270"
     CDS             200..634
                     /locus_tag="LAF92_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBI9794137.1"
                     /translation="MYGTCETLCRELAAKYPGNAPLMLVIWSPEEIQALADGMEISLT
                     DHEIRTVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARTLLKNNTHENN"
     gene            685..>904
                     /locus_tag="LAF92_005271"
     CDS             685..>904
                     /locus_tag="LAF92_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794138.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIY
                     VRLDAPRLATLLINRHGGVLPPLLASAIQ"
BASE COUNT          211 a          253 c          270 g          170 t
ORIGIN      
        1 gtggcggagg tggccaatgc catgtgcgac ctgcgtacaa ccatgaatga gatggagcag
       61 cggtacagct ttaatgccga caccctgccg gaacgtctgg tgcgtcagac gctgtttcgc
      121 gcaaaccgcc tcctgatgga ggcatatacc gaaattctgg aactggatgc gtgctttaaa
      181 gattgagaag gagacgagaa tgtacggaac atgcgaaacg ctatgccggg agctggcagc
      241 aaagtatccg ggaaacgcgc cgctgatgct ggttatctgg tccccggaag agattcaggc
      301 actggctgac ggtatggaaa tttctctgac cgatcatgaa atcaggaccg tcctggcgcg
      361 catggaggac atcccggaag accagcggac tgaatccggt atttcttccg ctgcggtgat
      421 ggagattatc agcaacgtga gcgaaaaccg ccaggtgacc gtccctgctg aactgctggc
      481 gtccctgatt cagaccgctg aacaggcact gtggaaacgt gagtgggccg cccgtgataa
      541 cggcctcgcc gtcccggaat gcgttacccg ccgtcaggcg gtggttaatc aggcccgcac
      601 cctgctgaaa aacaacacac acgaaaacaa ctgatgttat cgccgcctgc cctgcgagcg
      661 gcgatacagg gagaacgctt aatcatgaac gaaaacagca cgctgaacgc actgatttgt
      721 cgtcacgccc gtaacctgct gctggcgcag ggctggccgg aagagacgga tgttgaccag
      781 cgaaatccga actatccggg ctggatcagc atttacgtgc ggctggatgc gccccggctg
      841 gcgacgttac ttatcaaccg tcacggcggc gtactgccgc cgctcctggc ctccgccatt
      901 caga
//
LOCUS       DAEVZA010000348          885 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEVZA010000348 DAEVZA010000000
VERSION     DAEVZA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.358"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(1..155)
                     /locus_tag="LAF92_005272"
                     /pseudo
     CDS             complement(1..155)
                     /locus_tag="LAF92_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
     gene            complement(219..347)
                     /locus_tag="LAF92_005273"
     CDS             complement(219..347)
                     /locus_tag="LAF92_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794139.1"
                     /translation="MRYLLDIVSTDGYYWYMSGKICERVSDYRTAAFFEIGRLLTL"
     gene            complement(501..845)
                     /locus_tag="LAF92_005274"
                     /pseudo
     CDS             complement(501..845)
                     /locus_tag="LAF92_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FipA"
BASE COUNT          215 a          232 c          204 g          234 t
ORIGIN      
        1 tagttttccg acaacttcat gattacatat cttgcggttt tgattatttt tgctgcaaga
       61 aatacatact tcaaacgaaa ggtctttatt tgctgtctgt attctgaaga gtccaaggaa
      121 tcaaacttga acaacaaaaa taggttatat gaaatccgaa ttctgcggtt cccccttaaa
      181 caggtgtcgg gaaatttcgt tgagatattt tgaaggaatc acagtgtaag caggcggcct
      241 atttcaaaaa aggcggctgt acggtagtca ctgactctct cgcagatctt cccgctcatg
      301 taccagtaat acccgtcagt gctgacaata tcgagcaaat accgcatatc ataaaccgta
      361 tcgggtatta cctcaccctc accggccaga acgccgctgg caatagaatg gttccctcct
      421 cgtacaaagc ctatccgcca gggcaaccac aaagtaacgc ggtggttaat atcctgtacc
      481 cacggattgc ccttagcgct gcctatatcg gctaaagcac tccggtagct tgattcaccc
      541 cacggccacg gcaggatctt ggccgtcgca agcgccaggg gaaaatcttc agctgcaagc
      601 ctgagtgatt tcatgtgcgt gtaatccatc gcccagatga tttttgtgaa gaagaactcg
      661 cgctcgttca tgtccgggcg cgcgtcctgg ccctccgtgc caacggccag caagtaatcg
      721 gcctgaattg gcagtatcag cgcgcgtagt aagtcatgga tcgccggttg cggcagtctg
      781 tgcgccagat taattacccg gtcgaacttc agtttatcct gcttgcgctt gtcgatctgc
      841 tccatcagat ttcatggccc ccttcttcat gctcatgggt gtgtt
//
LOCUS       DAEVZA010000349          883 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEVZA010000349 DAEVZA010000000
VERSION     DAEVZA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.359"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..144)
                     /locus_tag="LAF92_005275"
     CDS             complement(<1..144)
                     /locus_tag="LAF92_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794140.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSA"
     gene            524..775
                     /locus_tag="LAF92_005276"
     CDS             524..775
                     /locus_tag="LAF92_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBI9794141.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
BASE COUNT          233 a          223 c          203 g          224 t
ORIGIN      
        1 ggcactcttt ttggcagctg agccactggt cggcaaatcg cggatgtgac cgacgctgga
       61 tttcaccacg tagtcactac ccagatactt gttgatcgtt ttggcttttg ccggggactc
      121 aacgatgaca agagctttac ccatattcac cttacctaat ttgattcttc caggaatgcg
      181 tcgcacgttg ataaaccttc cactggcaac gagttaccga tatggactcg ttctctgcgg
      241 atatcaaccc cttgtcgtga ctctgttcac gaaaattcag gtgactgagt taacaggtct
      301 tggcctacag gcgctatagc acgacggaat tttggtcgaa tgtcaagcaa atctattgcc
      361 agattgacga aagcgtcaca ctgtacctga taaaatccga tacgcaactt tattagcatg
      421 caaaatccaa agttaccagt aaggcgctct tgccaccagc gtggtatatt tgtctgcaag
      481 ccatgcctgg cgtagactaa tgtccttgtt taaggagtaa ataatgaaca aagagacaca
      541 acccatcgac cgcgaaacgc tgttgaaaga ggccaacaaa atcattcgtg agcatgagga
      601 tacgctcgca gggattgaag ccaccggcgt cacgcagcgt aatggcgtgc tggtatttac
      661 cggcgactac tttttagatg aacaaggatt gccaacagcc aaaagcaccg cggtgtttaa
      721 tatgttcaaa cacctggccc atgtgctttc agaaaaatat catctggttg attaaagcaa
      781 aacgcgaggt cttagcctcg cgtttgtctt ttacatcaat ggcttttgac cccgctgcca
      841 ccagcgtagc aacaatcgat cggcgctctc tgccgcgctg ccg
//
LOCUS       DAEVZA010000350          866 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEVZA010000350 DAEVZA010000000
VERSION     DAEVZA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.360"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..99)
                     /locus_tag="LAF92_005277"
     CDS             complement(<1..99)
                     /locus_tag="LAF92_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBI9794142.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGQDFLYAEA"
     gene            complement(516..>866)
                     /locus_tag="LAF92_005278"
     CDS             complement(516..>866)
                     /locus_tag="LAF92_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794143.1"
                     /translation="AALTTTGQTLLTRQTRRFRLVVKESDHPCWLDEDDENLPVVLDA
                     IVNRGARFSSVEMYLVSECIEHILSSGLACDVLRIPDEPPRRWFDRGVLREVVREART
                     EIRSMADALAKIRK"
BASE COUNT          200 a          259 c          265 g          142 t
ORIGIN      
        1 cgcttcggca tacagaaaat cctggccaaa caggtaaggc agaaaagaca gacggtcgca
       61 ctcttcaacg ttcagagtga caggtttcgc atattgcatg atgttttcct ccgttgttca
      121 catattccgc gacaccgggc ggtgccgtgc ctccataaaa gccgggcgat aaggcgggga
      181 tgcacagggc gcaagggaag ccgcagcctg acgcacacgg ctggcgggag ttggagggtg
      241 agaccgcgaa acctgagcgg gtgaacgacc tgaaaatcac ggcagacggg aaggagggac
      301 cgggtgaccg cagcggcgaa gcgtaccctt gcatcaacgc cagcccggct aagatggagg
      361 cacggcacag ccagcacacg acgctgaggc cacataactg aaaaaccaca aggccatgcc
      421 ctgcatggcc gggtaaagcg gagccggcgg cgacttcctt tgtgcctgtc cggggaccgg
      481 caggaaaaag gcggggattt accccgccag tgtcatcatt tccggatttt tgccagggca
      541 tccgccatgc tgcggatttc ggtccgggct tcccggacca cctcccgcag aacaccacgg
      601 tcaaaccagc ggcggggcgg ttcatccggt atacgcagca catcgcaggc cagcccactg
      661 gacaggatat gctcaataca ctcactgacc agatacattt ccaccgacga aaaacgtgct
      721 ccccggttca cgatggcatc gagcaccacg ggcaggtttt cgtcatcttc atcgagccag
      781 caggggtgat cgctttcctt cacgaccagg cgaaagcggc gcgtctggcg ggtcagcaac
      841 gtctgaccgg tggtggtcag cgccgc
//
LOCUS       DAEVZA010000351          859 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEVZA010000351 DAEVZA010000000
VERSION     DAEVZA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.361"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..186
                     /locus_tag="LAF92_005279"
     CDS             <1..186
                     /locus_tag="LAF92_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794144.1"
                     /translation="VAEVANAMCDLRTTMNEMEQRYSFNADTLPERLVRQTLFRANRL
                     LMEAYTEILELEACFKD"
     gene            200..634
                     /locus_tag="LAF92_005280"
     CDS             200..634
                     /locus_tag="LAF92_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBI9794145.1"
                     /translation="MYGTCETLCRELAVKYPGNAPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            685..>859
                     /locus_tag="LAF92_005281"
     CDS             685..>859
                     /locus_tag="LAF92_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794146.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLIN"
BASE COUNT          202 a          233 c          259 g          165 t
ORIGIN      
        1 gtggcggagg tggccaatgc catgtgcgac ctgcgtacaa ccatgaatga gatggagcag
       61 cggtacagct ttaatgccga caccctgccg gaacgtctgg tgcgtcagac gctgtttcgc
      121 gcaaaccgcc tcctgatgga ggcatatacc gaaattctgg aactggaagc gtgctttaaa
      181 gattgagaag gagacgagaa tgtacggaac atgcgaaacg ctatgccggg agctggcagt
      241 aaagtatccg ggaaacgcgc cgctgatgct ggttatctgg tccccggaag agattcaggc
      301 actggctgac ggtatggaaa tttctctgac cggtcatgaa atcaggaccg tcctggcacg
      361 cctggaggac atcccggaag accagcggat tgagtccggt atttcttccg ctgcggtgat
      421 ggagattatc cgcaacgaga gcgaaaaccg cctggtgacc gtccctgctg aactgctggc
      481 gtccctgatt cagaccgctg aacaggcatt gtggaaacgt gaatgggccg cccgggatca
      541 tggcctcgcc gtcccggaat gcgtcacccg ccgtcaggag gtggttaatc aggcccgcac
      601 cctgctgaaa aacaacacac acgaaaacga ctgatgttat cgccgcctgc cctgcgggcg
      661 gcgatacagg gagaacgctt aatcatgaac gaaaacagca cgctgaacgc actgatttgt
      721 cgtcacgccc gcaacctgct gctggcgcag ggctggccgg aagagacgga tgttgaccag
      781 cgtaacccga agtatccggg ctggatcagc atttatgttc tgctggatgc gccccggctg
      841 gcgacgttac ttatcaacc
//
LOCUS       DAEVZA010000352          835 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEVZA010000352 DAEVZA010000000
VERSION     DAEVZA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.362"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..390
                     /locus_tag="LAF92_005282"
     CDS             <1..390
                     /locus_tag="LAF92_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794147.1"
                     /translation="LLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAAL
                     VEAIRNDRMPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVH
                     VLTPIASVRRIVNMVALAVVEAQTQPL"
     gene            683..>835
                     /locus_tag="LAF92_005283"
     CDS             683..>835
                     /locus_tag="LAF92_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="ethanolamine utilization protein; involved in the
                     degredation of ethanolamine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutS"
                     /protein_id="HBI9794148.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGET"
BASE COUNT          204 a          197 c          210 g          224 t
ORIGIN      
        1 ctgttgtcgc actccaactt tggttcttct gactgcccgt cgtcgagcaa aatgcgtcag
       61 gcgctggaac tggtcaggga tcgtgcgcca gatctgatga ttgatggtga aatgcacggc
      121 gatgcagcgc tggtggaagc gattcgcaac gaccgtatgc cggacagccc tttgaaaggt
      181 tctgccaata ttctggtgat gccgaacatg gaagctgccc gcattagtta caacttactg
      241 cgcgtttcca gctcggaagg tgtgactgtc ggtccggtgc tgatgggcgt ggcgaaaccg
      301 gttcacgtgt taacgccgat cgcatcggtg cgtcgtatcg tcaacatggt ggcgctggcc
      361 gtggttgaag cgcaaaccca accgctgtaa ttctttttta actctcacgc ttaccctgaa
      421 tattcagggt aagcggtttg gctgcaatat attagtaaag cttattactg agtttgcgaa
      481 taataaaaaa agcagtctat ataatatctc gatattattt tatttatatt catgcgttgc
      541 atatgaaagt ttatgcacca cagcgaatat ctctccattc ttagtgatct acctcacctt
      601 ttaaacgcgc ttgccgaatt ttgttattta ctctgacgaa aaattgtcac gatacacgaa
      661 agtttttcac aggcggcgac tcatggataa agaacgcatc attcaggaat ttgtgcccgg
      721 caaacaggtc acactggcgc atctcatcgc gcatcccggt gaggaactgg cgaagaaaat
      781 cggcgttccg gatgcgggcg caattggcat tatgacgcta actcccggcg aaacg
//
LOCUS       DAEVZA010000353          827 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEVZA010000353 DAEVZA010000000
VERSION     DAEVZA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.363"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1..573
                     /locus_tag="LAF92_005284"
     CDS             1..573
                     /locus_tag="LAF92_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBI9794149.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            634..>827
                     /locus_tag="LAF92_005285"
     CDS             634..>827
                     /locus_tag="LAF92_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794150.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVT"
BASE COUNT          178 a          207 c          269 g          173 t
ORIGIN      
        1 gtgaaaacgg gggccgaagc catccgcgca ctggccacac agctcccggc gtttcgtcag
       61 aaactgagcg acggctggta tcaggtacgg attgccgggc gggacgtcag cacgtccggg
      121 ttaacggcgc agttacatga gactctgcct gatggcgctg tgattcatat tgttcccaga
      181 gtcgccgggg ccaagtcagg tggcgtattc cagattgtcc tgggggctgc cgccattgcc
      241 ggatcattct ttaccgccgg agccaccctt gcagcatggg gggcagccat tggggccggt
      301 ggtatgaccg gcatcctgtt ttctctcggt gccagtatgg tgctcggtgg tgtggcgcaa
      361 atgctggcac cgaaagccag aactccccgt acacagacaa cggataacgg caaacaaaac
      421 acctatttct cctcactgga taacatggtt gcccagggca atgttctgcc ggttctgtac
      481 ggtgaaatgc gcgtggggtc acgcgtggtt tctcaggaga tcagcacggc agacgaaggg
      541 gacggtggtc aggttgtggt gattggtcgc tgatgcaaaa tgttttatgt gaaaccgcct
      601 ccgggcggtt ttatcgttta tggagcatga cgaatgggta aaggcagcag taaggggcat
      661 accccgcgcg aagcgaagga caacctgaag tccacgcagc tgctgagtgt gatcgatgcc
      721 atcagcgaag ggccggttga aggtccggtg gatggattaa aaagcgtgct gctgaacagt
      781 acgccggtgc tggacagtga ggggaatacc aatatatccg gcgtcac
//
LOCUS       DAEVZA010000354          814 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEVZA010000354 DAEVZA010000000
VERSION     DAEVZA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.364"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>814
                     /locus_tag="LAF92_005286"
     CDS             <1..>814
                     /locus_tag="LAF92_005286"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBI9794151.1"
                     /translation="NRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDL
                     DREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDRVNAQQARRF
                     MDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTT
                     PSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFIT
                     STLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYI"
BASE COUNT          203 a          194 c          237 g          180 t
ORIGIN      
        1 caaccgtatg ggggttgacc cgtggcacaa ttgggaggcg cactatgaag tgttgcctgg
       61 taaagagaag gtcgtctctg aactgaaaca actggctgaa aaagccgacc acatctatct
      121 cgcaaccgac cttgaccgcg aaggggaagc cattgcatgg cacctgcggg aagtgattgg
      181 tggtgatgat gcgcgctata gccgagtggt gtttaacgaa attactaaaa acgcgatccg
      241 ccaggcattt aacaaaccgg gtgagctgaa tattgatcgt gttaatgccc agcaggcgcg
      301 tcgctttatg gaccgcgtgg ttgggtatat ggtttcgccg ctgctatgga aaaagatcgc
      361 tcgtggtctg tctgccggtc gtgtgcagtc ggtggcggtt cgcctggtgg tcgagcgtga
      421 gcgtgaaatt aaagcgttcg tgccggaaga gttctgggaa gtcgatgcca gcacgaccac
      481 gccatctggt gaagcgttgg cgttgcaggt gactcatcag aacgacaaac cgttccgtcc
      541 ggtcaacaaa gaacaaactc aggctgcggt aagtctgctg gaaaaagcgc gctacagcgt
      601 gctggaacgt gaagacaaac cgacaaccag taaacctggc gctcctttta ttacctctac
      661 gctgcaacaa gctgccagca cccgtcttgg atttggcgtg aaaaaaacca tgatgatggc
      721 gcagcgtttg tatgaagcag gctatatcac ttacatgcgt accgactcca ctaacctgag
      781 tcaggacgcg gtaaatatgg ttcgcggtta tatc
//
LOCUS       DAEVZA010000355          755 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEVZA010000355 DAEVZA010000000
VERSION     DAEVZA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.366"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(223..414)
                     /locus_tag="LAF92_005287"
     CDS             complement(223..414)
                     /locus_tag="LAF92_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794152.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(414..>755)
                     /locus_tag="LAF92_005288"
     CDS             complement(414..>755)
                     /locus_tag="LAF92_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBI9794153.1"
                     /translation="VLTRGDVRHMAQDWNLSDDELETVMQRLDDAFVYGACDRVVSDI
                     VNELMEEKRVNRLVTVPAVLLEKVMVMAGSEIYRLHAVGSENGGDGDAFVREEREIMR
                     VMRQALDGENG"
BASE COUNT          185 a          241 c          172 g          157 t
ORIGIN      
        1 ggcagacagg acaacactgc agtagtcaga atcagggcag cagagaaaag aacaacaaca
       61 acaggaacag ggtcatacca tcagaaaaaa acattccgga cacatcacaa acgggaaggg
      121 taacgggcga cgcgccagcg tggcccgctt cccttccggc aggcgtcagc ctgccagggt
      181 tttcgcgcgg gctgacaggc ggcctccacc gcctgccctg tatcacctgt ttctgctgcc
      241 ctgaaggata tagtgaccac cgaggaaaac cgcctgctga ttcgccacaa aatcgcagat
      301 ttcatcaaca atctgcgcag tggtgagcgg tttctgcccc atttcacgac gccgcgcatt
      361 aatcacggct ttgtaattgc gcagaatttc gcgggtttcc gtgctgatat tcattacccg
      421 ttctccccgt ccagcgcctg acgcatcacc cgcataatct cacgttcttc cctgacaaat
      481 gcgtcaccgt cgcccccgtt ctcgctccca acagcatgga ggcgataaat ttcactacca
      541 gccatcacca tcactttttc cagcagcacc gcagggactg ttacaagacg gtttacgcgc
      601 ttttcctcca tcagttcatt gacaatatcg gaaacaactc tgtcacatgc gccatataca
      661 aaagcatcgt ccagccgctg catgacggtt tccagctcat cgtctgacag gttccagtcc
      721 tgagccatat ggcgcacatc cccacgggtc agcac
//
LOCUS       DAEVZA010000356          740 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEVZA010000356 DAEVZA010000000
VERSION     DAEVZA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.367"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(62..>740)
                     /locus_tag="LAF92_005289"
     CDS             complement(62..>740)
                     /locus_tag="LAF92_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI9794154.1"
                     /translation="NELTEAAYYLSLKAKRVLWLCLMQTYFTASVSEDDDEMAVLGDS
                     TFKVKVADYQQIFQVSRNQAIKDVKEGVFELSRSAVIFYPKEGSFDCVARPWLTEAGS
                     RSARGIWEIEFNHKLLRYIYGLTNQFTTYSLRDCGSLRNPRTIRLYESLAQFKSSGLW
                     VTTHAWLNDRFLLPESQQKNLAELKRSFLDPALKQINEKTPLLAKYSIDDSGKFLFSI
                     IDKQNPV"
BASE COUNT          209 a          162 c          165 g          204 t
ORIGIN      
        1 aattcgccca tcttcatgac aaaaatttgt caaatgacag gctcatgtgt gctgatttat
       61 gtcagacggg attttgctta tcaattattg agaacagaaa ttttcctgaa tcatcaatac
      121 tatacttagc aagtaaaggt gttttctcat ttatctgctt gagagcagga tcaaggaaag
      181 atcgtttcaa ctctgccaag ttcttctgtt gggattccgg caaaaggaaa cggtcattta
      241 accaagcatg agtagtaacc cataagcctg aagatttgaa ttgagcaaga ctttcataaa
      301 ggcggatcgt ccgtggattt cgaagactgc cacaatcgcg gagcgagtag gtggtgaact
      361 ggttcgtcag gccgtaaatg taccgcagga gtttatggtt aaattcgatt tcccagatac
      421 cacgagctga tcggctgcca gcctctgtta gccaggggcg cgcgacgcag tcaaaactcc
      481 cttctttcgg gtaaaagatt accgcagaac ggcttaactc aaacacgcct tctttaacat
      541 ccttgatagc ctggttacgg cttacctgaa aaatttgctg atagtcagcc acctttactt
      601 tgaaagtaga gtcaccgagt acagccatct catcatcatc ttcgcttact gaagctgtga
      661 aatacgtctg cataagacat aaccagagaa cgcgctttgc ttttagcgag aggtagtaag
      721 cagcttcggt aagctcgtta
//
LOCUS       DAEVZA010000357          732 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEVZA010000357 DAEVZA010000000
VERSION     DAEVZA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.368"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..290)
                     /locus_tag="LAF92_005290"
     CDS             complement(<1..290)
                     /locus_tag="LAF92_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBI9794155.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRD"
     gene            complement(434..732)
                     /locus_tag="LAF92_005291"
                     /pseudo
     CDS             complement(434..732)
                     /locus_tag="LAF92_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
BASE COUNT          203 a          183 c          154 g          192 t
ORIGIN      
        1 cagtcacgca tccagttaat aaagcactcg tgaaattttg caggactgat acaggataca
       61 actctggcaa tggtatcgtg aacaggaata ccattttcaa aatcaccata ttgcttcaaa
      121 aaatcgagat gtgtttcccc aaaatcctct atatcttccc aaccctctgc accagaaata
      181 acggcacaaa tagtcaacag tagaatatcc gataatttat gctccacttt ccaggcttgt
      241 ctgtaatcgg ggataataga aatatgttcc atcaattttt taagttccat tttgttctcc
      301 ttaattatgt aaggagtatt tgatcatgta taagcaataa aaaacagctt caggtaataa
      361 ggaatatctc aatttttaaa cataaaatgc gaattattta gtgcaaaaag tgaacttctg
      421 atgatctttc cctggtcaac tgactgaggg cagcaacaat gttcgcaaaa tcctcttttg
      481 ccacttccgg gtagcgggca agactgtcga gcatcttttc ccctgccagc cactccagcg
      541 ccattttccg gctggccggt cgcgagggca gcagttgcgt accgaagcgt tccaccagtc
      601 cggccagcag cgccagcccc tcagccagtc cgcgctcacc gtcgcggtgc agtcgcgccc
      661 agatgtagta agtggcgatg cgcacgtctt ttgcctgcgt cagcagcagg gattcggcaa
      721 gctggcagat ga
//
LOCUS       DAEVZA010000358          722 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEVZA010000358 DAEVZA010000000
VERSION     DAEVZA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.369"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            24..100
                     /locus_tag="LAF92_005292"
     tRNA            24..100
                     /locus_tag="LAF92_005292"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="LAF92_005293"
     tRNA            143..218
                     /locus_tag="LAF92_005293"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
     gene            402..>722
                     /locus_tag="LAF92_005294"
     rRNA            402..>722
                     /locus_tag="LAF92_005294"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          208 a          153 c          196 g          165 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
      361 caacacgatg atgaatcgca agaaacatct tcgggttgtg aggttaagcg actaagcgta
      421 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      481 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      541 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      601 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      661 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgcgcg
      721 at
//
LOCUS       DAEVZA010000359          698 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEVZA010000359 DAEVZA010000000
VERSION     DAEVZA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.370"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..637)
                     /locus_tag="LAF92_005295"
     CDS             complement(<1..637)
                     /locus_tag="LAF92_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HBI9794156.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINI"
BASE COUNT          153 a          176 c          151 g          218 t
ORIGIN      
        1 ctatgttaat cgtacctctt tcgcctttgc gggtatgacg cctgccatgg cgaaggctat
       61 gcgaccgtcg cagatgctgt atattcaggt ggtgtagcgc ttcacggcta cgaaagtaca
      121 gcgttttata aattgtctca ggtgatattc gcagcgtttt ttgacgtggt tttgttcgcc
      181 ttaaccatcc tgatatttgc tctggagacc atttcatctc cagcttttcc agaacaagct
      241 ttcgcaatgg taaattttga tccagtaagc acggttttgg ccttttcgcc attctgttgg
      301 ctcggttatt agcatcaaca gctttgtaat agcgtctgcc ccgattacgc tgaacttcac
      361 gtgagatcgt cgaaggactg cgattcagcg cagtagctat cgcacgaatg ctcattttgg
      421 ctgacaaacc agctcgtatc tcctcgcgct cagacagtgt caggtgagct acagcccgct
      481 tacgctcatg gggttttatg ccgccagtat cccttaacat agtgaagatc gttccgggtt
      541 ttgaacccag gatattcgct atttcactga agcctgttcc gttcttccat agttcaaaaa
      601 cagaggcttt ttcctctgct gtaaatgttc gtctcattca aaaaacctcc gcaaccccat
      661 gttttcacat aactgttgcg ttgaccaatt gaatctac
//
LOCUS       DAEVZA010000360          698 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEVZA010000360 DAEVZA010000000
VERSION     DAEVZA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.371"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(284..496)
                     /locus_tag="LAF92_005296"
     CDS             complement(284..496)
                     /locus_tag="LAF92_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794157.1"
                     /translation="MNGFRNSSQNGQVWCWQRAGSRAVILEVSGRWMEAAEGWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(629..>698)
                     /locus_tag="LAF92_005297"
     CDS             complement(629..>698)
                     /locus_tag="LAF92_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBI9794158.1"
                     /translation="RLDKIRRQNRIKAELQAVLDEK"
BASE COUNT          171 a          190 c          130 g          207 t
ORIGIN      
        1 tgaaaagata aataatacca gtggaatata tttcctcatg ttattctcca tatcagataa
       61 atagcacctg tatttcaggc atcctgaata ttgcagcaca cactgaataa caccttattt
      121 tgtgaaatta atcacaaaat ataaaatcac gacttaatac ggaaaaaact ctgtgctact
      181 cagaactgtc cggtcatact gcccgaccgg atattaaaaa aagcaaatct catgcattat
      241 tcagccttct gaatccctgg agtaatgaag gctgcttttt tcgttacacc cttccccggc
      301 agcgccggtg gcagtgttca gccctttttc gggcaaactg ctgccagtct gtccggggcg
      361 caacacaggc agcccgtcgc catccttctg ccgcttccat ccagcgtcca ctgacctcca
      421 gaataacggc ccggcttccg gcacgctgcc agcaccagac ctgaccgttc tgtgaactgt
      481 ttctgaatcc attcatcttt atcactcctc tttaacccgt cagtattatg gtgttaaata
      541 atctactgaa ttggcttcct ttcgggtacg gaagttattt ccttttaagc cttatttccc
      601 tgcctcctct ctctgaggga ggcttttttt atttctcatc aagcacggcc tgaagttctg
      661 cctttatccg gttctggcgg cggattttat ccagacgc
//
LOCUS       DAEVZA010000361          686 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEVZA010000361 DAEVZA010000000
VERSION     DAEVZA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.372"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>686
                     /locus_tag="LAF92_005298"
     rRNA            <1..>686
                     /locus_tag="LAF92_005298"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          155 c          226 g          130 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaacc
//
LOCUS       DAEVZA010000362          684 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEVZA010000362 DAEVZA010000000
VERSION     DAEVZA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.373"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            174..>684
                     /gene="yddG"
                     /locus_tag="LAF92_005299"
     CDS             174..>684
                     /gene="yddG"
                     /locus_tag="LAF92_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBI9794159.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWA"
BASE COUNT          177 a          151 c          171 g          185 t
ORIGIN      
        1 atctttttgc gcgaaatcaa aaagaacacg tgtttgccac aaaataatcc gcctgcgcaa
       61 attgacctac ctcaatagcg gtagaaaaac gcaccactgc ctgacagacc agttaaaaaa
      121 atgctataaa attcagctta atttttaacg gcaagagaga caaaacagcg agcatgacac
      181 gacaaaaagc aacgctcata gggctaatag cgatcgtcct gtggagcacg atggtaggat
      241 tgattcgcgg tgtcagcgag gggctcggcc cggtcggcgg cgcagctgct atctattcat
      301 taagcgggct gctgttaatc ttcacggttg gatttccgcg tattcggcag attccgaaag
      361 gctatttact cgccgggagt ctgttattcg tcagctatga aatctgtctg gcgctttcct
      421 tagggtatgc ggcgacccgt catcaggcga ttgaagtggg tatggtgaac tatctatggc
      481 ccagcctgac aattctcttt gccattctgt ttaatggtca gaaaaccaac tggctgattg
      541 taccaggatt attattagcc ctggtcggcg tctgctgggt gttaggcggt gataatgggt
      601 tacattatga tgaaatcatc aataatatta ccaccagccc attgagttat ttcctggcgt
      661 ttattggtgc gtttatctgg gcag
//
LOCUS       DAEVZA010000363          681 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEVZA010000363 DAEVZA010000000
VERSION     DAEVZA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.374"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            41..681
                     /locus_tag="LAF92_005300"
                     /pseudo
     CDS             41..681
                     /locus_tag="LAF92_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          177 a          193 c          181 g          130 t
ORIGIN      
        1 cgcggctaag tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa cagtatcaac
       61 cagtaaaaaa ccccgtaaac agcattcgcc tgaatttcgc agtgaagccc tgaagcttgc
      121 tgaacgcatc ggtgttactg ccgcagcccg tgaactcagc ctgtatgaat cacagctcta
      181 caactggcgc agtaaacagc aaaatcagca gacgtcttct gaacgtgaac tggagatgtc
      241 taccgagatt gcacgtctca aacgccagct ggcagaacgg gatgaagagc tggctatcct
      301 ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga agtatgtctt tattgaaaaa
      361 catcaggctg agttcagcat caaagcaatg tgccgcgtgc tccgggtggc ccgcagcggc
      421 tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc gtcagcagtt ccgccaacac
      481 tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac agcgttacgg tgccccacgc
      541 ctgacggatg aactgcgtgc tcagggttac ccctttaacg taaaaaccgt ggcggcaagc
      601 ctgcgccgtc agggactgat ggcaaaggcc tcccggaagt tcagcccggt cagctaccgc
      661 gcacacggcc tgcctgtgtc a
//
LOCUS       DAEVZA010000364          679 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEVZA010000364 DAEVZA010000000
VERSION     DAEVZA010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.375"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>679
                     /locus_tag="LAF92_005301"
     CDS             <1..>679
                     /locus_tag="LAF92_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBI9794160.1"
                     /translation="IPAVVVPVSLIGTFAAMYLCGFSLNNLSLMALTIATGFVVDDAI
                     VVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLLLMGGLPGRLLRE
                     FAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRMLVALQQGYGKSLK
                     WVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQADQSISFQAMRGK
                     LQDFMKII"
BASE COUNT          126 a          163 c          207 g          183 t
ORIGIN      
        1 attcccgccg ttgtggtgcc ggtttcgctg attggtacgt ttgcggcgat gtacctgtgc
       61 ggattcagtc tcaataacct gtcgttaatg gcgctcacca tcgctactgg tttcgtggtg
      121 gatgacgcca tcgtggtgct ggaaaacatt gcccgtcatc tggaagcggg gatgaaacca
      181 ctacaggccg cgctacaagg tactcgcgaa gtcggcttta cggtgctgtc gatgagtctg
      241 tcactggtgg cggtgttcct gccgctgctg ttgatgggcg gattgccggg ccgactgtta
      301 cgcgagtttg ccgtgacgct ttctgtcgcc attggtattt cgttactggt ttctctgaca
      361 ttaacgccaa tgatgtgtgg ctggatgctg aaagccagca agccgcgcga gcaaaagcga
      421 ctacgtggtt ttggtcgcat gttggtagcc ctgcaacaag gctacggcaa gtcactgaaa
      481 tgggtgctca atcatacccg tctggtgggc gtggtgctgc ttggcaccat tgcgctgaat
      541 atctggctgt atatctcgat cccgaaaacc ttcttcccgg agcaggacac tggcgtgttg
      601 atgggcggga ttcaggcgga tcagagtatt tcgtttcagg cgatgcgcgg taagttgcag
      661 gatttcatga aaattatcc
//
LOCUS       DAEVZA010000365          679 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEVZA010000365 DAEVZA010000000
VERSION     DAEVZA010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.376"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>679)
                     /locus_tag="LAF92_005302"
     CDS             complement(<1..>679)
                     /locus_tag="LAF92_005302"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Has polymerase, DNA-binding and 3'-5' exonuclease
                     activities. In Aeropyrum pernix this protein is sensitive
                     to aphidicolin and stable at 95#C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBI9794161.1"
                     /translation="YVASRPLLLEKLNAWFANHDPDVIIGWNVVQFDLRMLQKHAERY
                     RLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIIDGIEALKSAFWNFSSFSLETVAQ
                     ELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLKDCELVTQIFHKTEIMPFLLERA
                     TVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLGEVPPHASPGGYVMDSRPGLYDS
                     VLVLDYKS"
BASE COUNT          159 a          189 c          178 g          153 t
ORIGIN      
        1 ggcttttata gtccagcacc agcactgaat cataaagccc tggccgtgaa tccatcacgt
       61 agccgccagg gctggcgtgc ggtggcactt cgccgagatt aggcgcgaca taaccagcgc
      121 gatgcattcg cggaaaatag agatgaccaa acgctgccac cgaaccgccg tgccgatcca
      181 ccggcaggcc gttcaccgtt gctcgttcga gtaaaaatgg catgatttca gttttgtgga
      241 agatctgcgt caccagctcg caatctttca ggttataagt tgccagcgca ggtttatctt
      301 cggcgaaacg gcggtcaatt tcgtccattc gatcccacgg gttatcgata gattttcctt
      361 cgcctaatag ctcctgagcg acagtttcca gcgagaatga agagaaattc cagaacgcgg
      421 atttcagcgc ttcgataccg tcgataatta gccgaccttt agcctgggca aaaaagacgc
      481 cgtttttaaa gccgtgctcg cgccactcca gctcgctatt atcgcgccca agacgcagcg
      541 gaagacggta acgctcggca tgtttttgca gcattcgcag atcgaactgc accacgttcc
      601 agccgatgat cacatcggga tcgtggtttg caaaccaggc gttgagtttt tccagcagca
      661 gcgggcggct ggcgacgta
//
LOCUS       DAEVZA010000366          669 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEVZA010000366 DAEVZA010000000
VERSION     DAEVZA010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.377"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..86
                     /locus_tag="LAF92_005303"
     CDS             <1..86
                     /locus_tag="LAF92_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBI9794162.1"
                     /translation="FRERLCELNIHRGNTVVQVTLAYEARK"
     gene            370..627
                     /gene="repA"
                     /locus_tag="LAF92_005304"
     CDS             370..627
                     /gene="repA"
                     /locus_tag="LAF92_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBI9794163.1"
                     /translation="MSQTENAVTSSSGKKRPYRRGNPVPARERQKASLARRSATHKAF
                     HAVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
BASE COUNT          187 a          141 c          175 g          166 t
ORIGIN      
        1 tattcaggga acggttatgt gaactgaata ttcacagggg aaatacagtg gtgcaggtaa
       61 ctctggccta cgaagcacgg aagtaagctg ccgggcgggg acggaagtcc ccgctttccg
      121 gaagtgtgag gtatttcagg ggcagacacc cgacatgcca gaaacagccg gtcccgcccg
      181 gggccggcat cctggttaag gcatttcctg cttttcagtc atttcattat caaaatcaca
      241 ttaaacggtt gtaatcagac atgatttgtg cgccaacacc gatcatcgtc acaactttca
      301 agtcgctgat ttcaaaaaac tgtagtatcc tctgcgaaac gatctctgtt tgattattga
      361 ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct catcgggaaa aaaacgacct
      421 tacagaaggg gtaatcctgt tcctgcgaga gaacgacaaa aagcgtctct tgcaagaagg
      481 agtgccactc ataaagcgtt tcatgccgtc attcagcttc gactgaaaga aaagttaagt
      541 gagcttgctg atgaagacgg gatcactcag gcacagatgc ttgagtggct gatagagtca
      601 gaggttaagc gcagaaaatc tttgtgagta tttgcgtttc ttgctgtttc agtgatgaga
      661 tgttagatt
//
LOCUS       DAEVZA010000367          641 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEVZA010000367 DAEVZA010000000
VERSION     DAEVZA010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.378"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>641
                     /locus_tag="LAF92_005305"
     CDS             <1..>641
                     /locus_tag="LAF92_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI9794164.1"
                     /translation="VAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITIT
                     DSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDV
                     VTGLITRITTPDGRASAFYYNHHNQLTSATGPDGLELRREYDESGRLIQETAPDGDIT
                     RYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLSFTDCSGYQTRYGHDRFGQ"
BASE COUNT          154 a          196 c          195 g           96 t
ORIGIN      
        1 tggtggcgca ccgtcacacg ggccggccgg aaatccgtta ccgttacgac agcgacgggc
       61 gggtgacaga acagctaaac ccggcaggct taagctacac gtatcagtat gagaaagacc
      121 gcatcaccat caccgacagc ctggaccgcc gtgaagtgct gcacacgcag ggcgaagccg
      181 ggctgaagcg ggtggtgaaa aaggaacacg cggacggcag cgtcacgcag agtcagtttg
      241 acgccgtggg caggctcagg gcacagacgg atgccgcagg caggacaaca gagtacagcc
      301 cggatgtggt gacgggcctc atcacgcgca taaccacgcc ggatggcagg gcatcggcgt
      361 tttactataa ccaccacaac cagttaacgt cagccaccgg gcctgacggg ctggaattgc
      421 gccgggaata tgatgaatcc ggccgtctga ttcaggaaac cgcccctgac ggcgatatca
      481 cccgctaccg ttatgataat ccacacagtg acctcccctg cgcaacggac gatgccaccg
      541 gcagccggaa aaccatgacg tggagccgtt acggccagtt gctgagcttc accgactgtt
      601 ccggctacca gacccgttat ggccatgacc gcttcgggca g
//
LOCUS       DAEVZA010000368          622 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEVZA010000368 DAEVZA010000000
VERSION     DAEVZA010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.379"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>622
                     /locus_tag="LAF92_005306"
     CDS             <1..>622
                     /locus_tag="LAF92_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI9794165.1"
                     /translation="AVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDLTAT
                     VSPDGKRSTIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDR
                     LTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYD
                     DHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTDGEDGPHPDGCY"
BASE COUNT          151 a          172 c          206 g           93 t
ORIGIN      
        1 cgccgttcac cgggaagaag gcatcagcac ttacagcagt tataacccgc gcggtcaact
       61 ggtcagtcag agggatgcgc aggggcggga gacccgttat gagtacagcg ccgcaggcga
      121 cctgacagca acggtatcac cggacggtaa acgcagcacc atcgaatatg ataaacgcgg
      181 gcgtccggtg agtgtgacgg aaggcggtct gacgcgcagc atggggtatg acgctgccgg
      241 gcgcatcact gtgctgacca acgagaacgg cagccagtcc acgttccggt atgacccggt
      301 cgacaggctg acggagcagc gcggctttga tggcaggacg caacgttacc agtatgacct
      361 gaccggaaaa ctcacgcaga gtgaagacga gggacttatc accctctggc attacgatgc
      421 gtcggaccgt atcacccggc gcacggtgaa cggtgaaccg gcggagcagt ggcagtatga
      481 cgaccacggc tggctgacag aaatcagcca cctgagtgaa ggccaccgtg tggcggtcca
      541 ctacggctat gacgataaag gccgtctgac gggcgaacgg cagactgacg gagaagacgg
      601 accgcatccc gacgggtgtt at
//
LOCUS       DAEVZA010000369          599 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEVZA010000369 DAEVZA010000000
VERSION     DAEVZA010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.380"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>599)
                     /locus_tag="LAF92_005307"
     CDS             complement(<1..>599)
                     /locus_tag="LAF92_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794166.1"
                     /translation="SIAVDTAHFESTSGATSNHVQSSDVFIARSHHGSVSKEQRAITE
                     QALKSGELRCVVATSSLELGIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKG
                     LFFPRTRRDLVDSAVIVECMFAGRLENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYS
                     RVRRAAPWKDLPRRVFDATLDMLSGRYPSGDFSAFRPKL"
BASE COUNT          143 a          164 c          172 g          120 t
ORIGIN      
        1 cagtttgggg cgaaaagcag aaaaatcgcc agagggatag cgcccggaaa gcatatccag
       61 cgtggcgtca aaaacacgtc ttggcagatc tttccacggt gcggcacggc gtacgcggga
      121 gtaccattcg tctacctgta atgcctccat cgccgcggcg gcaacggttt gctgcgcaag
      181 gacgtcgagt ggattatgcg gcggtgtcag gttttccagc ctgcctgcga acatacactc
      241 tacaatgact gcggaatcga ctaagtcacg ccgggtacga gggaaaaaca gccctttaga
      301 tacaccgcca acctgatgtc cggcgcgacc aatgcgttgt aacccactgg caacagaaag
      361 cggcgttgcc acctgaatca ccagatccac cgcgcccata tcaatcccca gttcgagact
      421 ggaggttgcg accacgcagc gtaattcacc cgatttcagc gcctgttcgg tgattgctcg
      481 ttgttcttta gagacggagc cgtggtgtga gcgggcaata aaaacgtcgc tactttgtac
      541 atggttagag gttgcgccgg aggtcgactc gaaatgggcc gtatcaacgg cgatagacg
//
LOCUS       DAEVZA010000370          598 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEVZA010000370 DAEVZA010000000
VERSION     DAEVZA010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.381"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>598
                     /locus_tag="LAF92_005308"
     CDS             <1..>598
                     /locus_tag="LAF92_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HBI9794167.1"
                     /translation="NALESRLAQSGGMNAVVPQNSSSIPAASSPVDAPQVQTITSGRD
                     LLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKTRLVPPRTESRNQL
                     KRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGVPWDRWTAVIRQDLA
                     LLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDG"
BASE COUNT          123 a          175 c          203 g           97 t
ORIGIN      
        1 aacgcgctgg aatcgcgtct ggcgcagtcg ggtgggatga acgcggtggt gccgcaaaac
       61 agcagcagca tacctgcagc gtcatccccg gtggatgcgc cacaggtaca gaccatcacc
      121 tccgggcgcg acctgctcga ccaggcgaaa gtgctggccc gttatctgaa cgaacagccg
      181 cagggctggc tttcggcgca ccggctgatg aaaaccctgc gctgggatac ggtgcatgaa
      241 ctgccgccgg atgtggacgg caaaacccgg ctggtcccgc cgcgcaccga gtcacgcaat
      301 cagcttaagc ggctgtatgc ccagcagaac tggacggaac tgctggagca ggccgacctg
      361 atgttcagca ctggcgtcag ccatttctgg ctggacattc agtggtatct gcatcaggcg
      421 ctgactaagg cgggcgtacc gtgggacagg tggacagcgg ttatccgtca ggacctggcg
      481 cttctgctgg agcgcctgcc ggggctggaa aatctggcat ggaacgacgg cacaccgttc
      541 gccgacgagg tgacgcgcaa ctggatagcg cagcaggtca tgatgcggga agacggcg
//
LOCUS       DAEVZA010000371          598 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEVZA010000371 DAEVZA010000000
VERSION     DAEVZA010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.382"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1..598
                     /locus_tag="LAF92_005309"
                     /pseudo
     CDS             1..598
                     /locus_tag="LAF92_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          138 a          159 c          169 g          132 t
ORIGIN      
        1 gccggacatc agcgctatct ctgctctcat tgccgtaaaa catggcaact acagttcact
       61 tacaccgcct ctcagcccgg tacgcaccag aaaatcattg atatggccat gaatggcgtc
      121 ggatgtcgcg ccagtgcacg cattattggc gttggcctca acacgatttt ccgccattta
      181 aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagggac gtcatcgtct
      241 gcgcggaaat ggacgaacag tggagctacg tcggggctaa atcacgccag cgctggctgt
      301 tttacgcgta tgacagggta cgcaggactg tcgtggccca cgtttttggt gaacgcacaa
      361 tgttcacgct ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg gtatggatga
      421 cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgtt atcagcaagc
      481 gttacactca gcgcattgag cgacataatc tgaatctgag acaacatctg gcaaggctgg
      541 gacggaagtc actgtcgttc tcaaaatcgg tggagctgca tgacaaggtc atcgggca
//
LOCUS       DAEVZA010000372          591 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEVZA010000372 DAEVZA010000000
VERSION     DAEVZA010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.383"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..419
                     /locus_tag="LAF92_005310"
     CDS             <1..419
                     /locus_tag="LAF92_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBI9794168.1"
                     /translation="VCLSWGVNHYRDNAIAYKEQRDKNARELKLANAAITDMQMRQRD
                     VAALDAKYTKELADAKAENDALRDDVAAGRRRLRIKAVCQSVREATATSSVDNAVSPR
                     LADTAERDYFTLRERLVMMQAQLEGAQQYITEQCLK"
BASE COUNT          179 a          121 c          141 g          150 t
ORIGIN      
        1 tcgtctgcct gtcatggggt gttaatcatt accgtgataa cgccatcgcc tacaaagaac
       61 agcgcgacaa aaatgccaga gaactgaagc tggcgaacgc ggcaattact gacatgcaga
      121 tgcgtcagcg tgatgttgct gcgctcgatg caaaatacac gaaggagtta gctgatgcga
      181 aagctgaaaa tgatgctctg cgtgatgatg ttgccgctgg tcgtcgtcgg ttgcgcatca
      241 aagcagtctg tcagtcagtg cgtgaagcca ccgccacctc cagcgtggat aatgcagtct
      301 cccccagact ggcagacacc gctgaacggg attatttcac cctccgggaa cgactggtaa
      361 tgatgcaggc ccaacttgaa ggtgctcagc aatacataac cgaacagtgt ttaaagtaaa
      421 atcttaacta caatatgatt cattttgatg attgtttcat aaggaacagt gaagtaagat
      481 ctaagaggag ttaaatttta tacagtataa tcataatatt gcagcaaggt ggttataatt
      541 gaaagaatat ttagatatga atacatctca tgtaagagtt gttactcata t
//
LOCUS       DAEVZA010000373          587 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEVZA010000373 DAEVZA010000000
VERSION     DAEVZA010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.384"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..555
                     /locus_tag="LAF92_005311"
     CDS             <1..555
                     /locus_tag="LAF92_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBI9794169.1"
                     /translation="IVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIE
                     VGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQED
                     QLGALGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSF
                     TLAELVTKGHLRPLKEASEAENVA"
BASE COUNT          156 a          146 c          154 g          131 t
ORIGIN      
        1 atagtccttc agtgggacga aatgatccgg accgctggct ccctgaagct gggcaaagta
       61 caggcttcag tgctggtccg ttcattgctg aaaagtgaac gtccttccgg actgactcag
      121 gcaatcattg aagtggggcg catcaacaaa acgctgtatc tgcttaatta tattgatgat
      181 gaagattacc gccggcgcat tctgacccag cttaatcggg gagaaagtcg ccatgccgtt
      241 gccagagcca tctgtcacgg tcaaaaaggt gagataagaa aacgatatac cgacggtcag
      301 gaagatcaac tgggcgcact ggggctggtc actaacgccg tcgtgttatg gaacactatt
      361 tatatgcagg cagccctgga tcatctccgg gcgcagggtg aaacactgaa tgatgaagat
      421 atcgcacgcc tctccccgct ttgccacgga catatcaata tgctcggcca ttattccttc
      481 acgctggcag aactggtgac caaaggacat ctgagaccat taaaagaggc gtcagaggca
      541 gaaaacgttg cttaacgtga gttttcgttc cactgagcgt cagaccc
//
LOCUS       DAEVZA010000374          579 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEVZA010000374 DAEVZA010000000
VERSION     DAEVZA010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.385"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>579
                     /locus_tag="LAF92_005312"
     rRNA            <1..>579
                     /locus_tag="LAF92_005312"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          163 a          127 c          177 g          112 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcac
//
LOCUS       DAEVZA010000375          578 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEVZA010000375 DAEVZA010000000
VERSION     DAEVZA010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.386"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1..578
                     /locus_tag="LAF92_005313"
                     /pseudo
     CDS             1..578
                     /locus_tag="LAF92_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          214 a           79 c          139 g          146 t
ORIGIN      
        1 accattgatg ctatgggctg tcagaaagat atcgcttcga agatcaaaga taaaaaagca
       61 gattatcttc tggcagtaaa aggcaatcag gggaaattac atcatgcatt cgaggaaaaa
      121 tttcctgtaa atgtgttttc taattataaa ggcgattcgt ttagtacgca ggagataagt
      181 catggaagaa aagaaacacg tttgcatatt gtcagtaacg taacgcctga acttttgtga
      241 ttttgaattc gaatggaagg gattaaaaaa gctttgtgta gcattgtcat tcaggcagaa
      301 gaaagaagat aaatcagcag aaggtgtaag catccgatat tatatttcat caaaggatat
      361 ggatgctaaa gaatttgcac atgctatcag agcgcactgg ctgatcgagc acagtcttca
      421 ttgggtgtta gatgtaaaaa tgaatgaaga tgccagccgg ataagaagag gaaacgcagc
      481 ctaaataata tctggaataa agaagatggc actgaattta ttaagggatt gcaaagacat
      541 taagggtgaa gaggaaaaga aagaaggctg cgttaaac
//
LOCUS       DAEVZA010000376          578 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEVZA010000376 DAEVZA010000000
VERSION     DAEVZA010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.387"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>578
                     /locus_tag="LAF92_005314"
     CDS             <1..>578
                     /locus_tag="LAF92_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9794170.1"
                     /translation="RAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDE
                     SSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEI
                     VRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKL
                     AEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRT"
BASE COUNT          124 a          162 c          196 g           96 t
ORIGIN      
        1 cgtgcggtcg gggagatgcg cagcgccggc gtggatgcac agacactggc gtcctttctg
       61 catgacacgc aactgcagca gcgcagcgga gaaacgccgg atttcagcaa cacgctgttc
      121 ctgctcgatg agagctcaat ggtgggcaat accgacatgg cacgggcata cgccctgatt
      181 gcggccggtg gtggtcgtgc cgtggccagc ggtgacacgg accagctgca ggccatcgcg
      241 cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggc catcatgaag
      301 gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat taaccgggat
      361 gtggaaaggg cactgtccgg gcttgagagt gtgaaaccgt ctcaggtgcc acgtcaggag
      421 ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga agcaaaactg
      481 gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt tcccggatgt ccccatgaca
      541 ctgtatgaag ccattgtccg cgactatacc ggcaggac
//
LOCUS       DAEVZA010000377          578 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEVZA010000377 DAEVZA010000000
VERSION     DAEVZA010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.388"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..555
                     /locus_tag="LAF92_005315"
     CDS             <1..555
                     /locus_tag="LAF92_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794171.1"
                     /translation="RGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSS
                     FDYGTKSTNNTGAHTHSISGTANSAGAHQHKSSGAFGGTNTSIFPNGYTAISNLSAGI
                     MSTTSGSGQTRNAGKTSSDGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTI
                     TVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          160 a          152 c          148 g          118 t
ORIGIN      
        1 cgtggctgga cgattaaggg caaacctgcc agtggtcggg ccgtattgtc tcaggaacag
       61 gacggcatta aatcgcacac ccacagcgcc agcgcatcca gtacggattt ggggacgaaa
      121 accacatcgt cgtttgatta cggaaccaaa tccacgaata acaccggggc gcatacccat
      181 agtattagcg ggaccgcaaa tagtgccggt gcgcaccaac acaagagttc cggtgcattt
      241 ggtggcacga acacgagcat tttccctaat ggttataccg cgatttcaaa tctaagcgcg
      301 gggattatga gcacaacaag cggtagtggc cagactcgta atgcagggaa gacatcatca
      361 gatggtgctc atacccactc gctgtccggc actgctgcaa gcgcaggcgc acatgcacat
      421 actgtcggta ttggtgctca tacgcactcc gttgcgattg gctcacatgg acacaccatc
      481 accgttaacg ctgctggtaa cgcggaaaac accgtcaaaa acatcgcatt taactatatt
      541 gtgaggcttg cataatggca ttcagaatga gtgaacaa
//
LOCUS       DAEVZA010000378          562 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEVZA010000378 DAEVZA010000000
VERSION     DAEVZA010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.389"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..378
                     /gene="trbI"
                     /locus_tag="LAF92_005316"
     CDS             <1..378
                     /gene="trbI"
                     /locus_tag="LAF92_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBI9794172.1"
                     /translation="TQKTTDVTARRCSHWWWTVPGCLAMVLLNAAVSYGIVRLNAPVT
                     VAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVSPAV
                     VQGAPDITREIQQDIARRMRAEP"
     gene            375..>562
                     /locus_tag="LAF92_005317"
     CDS             375..>562
                     /locus_tag="LAF92_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794173.1"
                     /translation="MRCRGLIALLIWGQSVTAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERV"
BASE COUNT          128 a          144 c          186 g          104 t
ORIGIN      
        1 acgcaaaaaa cgactgatgt tacagcccgt cgatgcagcc actggtggtg gacggtgccg
       61 ggatgcctgg caatggtgtt actgaacgca gccgtcagtt acggcattgt cagactgaac
      121 gcaccggtga ctgtcgcctt caacatgaag cagaccgtgg atgcgttttt tgacagcgcc
      181 agtcagaaac aactgtcaga agcacagtca aaagcccttt cagcgcgttt taatacggca
      241 ctggaagcca gcctgcaggc gtggcagcag aaacaccacg cggtcattct ggtgtcgcct
      301 gccgtggtac agggggcacc ggatatcacc cgggaaatcc agcaggatat cgcccggcga
      361 atgagggcgg aaccatgaga tgccgggggc tgattgccct gctgatatgg gggcagagtg
      421 tgaccgccgc cgatcttggt acctggggcg atctgtggcc ggtaaaggag ccggacatgc
      481 tgacggtgat catgcagcgc ctgacggcac tggagcagtc cggtgagatg ggccggaaaa
      541 tggatgcctt taaggagcgg gt
//
LOCUS       DAEVZA010000379          549 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEVZA010000379 DAEVZA010000000
VERSION     DAEVZA010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.390"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>549)
                     /locus_tag="LAF92_005318"
     CDS             complement(<1..>549)
                     /locus_tag="LAF92_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI9794174.1"
                     /translation="ARRCAPAESCGFVVRTPEGERYFPCVNISGEPEAYFRMSPEDWL
                     SAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHKFRCVPHLTGRR
                     FEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEATGLYQVPLSVAQ
                     PGDVLLCCFGSSVPNHAAIYCGD"
BASE COUNT          127 a          198 c          132 g           92 t
ORIGIN      
        1 gtcgccgcag taaattgcgg cgtgattcgg cactgatgaa ccaaaacagc acagcagcac
       61 atcgcccggc tgtgccactg acaacggcac ctgatacagc cccgtcgcct ccagattatc
      121 cagatagaga ttctggccgt tacgccacca gtcatcctca cgataaaagt ccggcatctc
      181 aatccccgcc agatgataag catcccggaa cagtgtgtaa cagtccgtca caccgtgctc
      241 aaagcgccgc ccggtgagat gcggcacaca gcggaactta tgaatcgtcc cccggcagac
      301 cagccaccac ggcaaatcac tctgcacctg cagccgccgg tcggcctcac tcagccaggg
      361 cagaccaccg gggtggctgt ggaccagcgc cacaatctct ccctgcattt ctgcactcag
      421 ccagtcctcc ggcgacatcc ggaaatacgc ctccggctca ccagagatat tcacgcaggg
      481 aaaatatctt tccccctccg gtgttctcac cacgaagccg cacgactccg ctggcgcaca
      541 tcgccgggc
//
LOCUS       DAEVZA010000380          544 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEVZA010000380 DAEVZA010000000
VERSION     DAEVZA010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.391"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            19..>544
                     /locus_tag="LAF92_005319"
     CDS             19..>544
                     /locus_tag="LAF92_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBI9794175.1"
                     /translation="MDLRVKTGAEAIRALATQLPVFRQKLNEGWYQVRIAGRDAGENE
                     LSARLNEPLANGAVIHIVPRLAGAKSGGVFQVVLGAALIAVAWWNPVGWLGAAAVSGM
                     YAAGASMILGGVAQMLAPKARTPTAASTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMR
                     VGSRVVSQEISTADE"
BASE COUNT          109 a          134 c          189 g          112 t
ORIGIN      
        1 caacgatttg gtcgccgtat cgaccttcgt gtgaaaacgg gggctgaagc catccgggca
       61 ctggccacac agctcccggt gtttcgtcag aaactgaatg agggctggta tcaggtgcgc
      121 attgccgggc gtgatgcagg cgaaaatgaa ttatctgccc gtcttaatga gccgctggca
      181 aatggtgccg tgatccacat cgtgccgcgt ctggcgggag ctaaaagtgg cggtgtgttt
      241 caggtggtgc ttggggcggc gctgattgcg gtggcatggt ggaaccctgt gggctggctg
      301 ggggccgcgg ctgtatcggg catgtatgcg gcaggggcca gtatgatcct gggcggagtg
      361 gcgcagatgc tggcaccgaa agccaggacg cccacggcag ccagtacaga taacggcaaa
      421 cagaacacct atttctcctc actggataac atggttgccc agggcaatgt tctgccggtt
      481 ctgtacggtg aaatgcgcgt ggggtcacgc gtggtttctc aggaaatcag cacggcagac
      541 gaag
//
LOCUS       DAEVZA010000381          532 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEVZA010000381 DAEVZA010000000
VERSION     DAEVZA010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.392"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            86..334
                     /locus_tag="LAF92_005320"
     CDS             86..334
                     /locus_tag="LAF92_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794176.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(331..>532)
                     /locus_tag="LAF92_005321"
     CDS             complement(331..>532)
                     /locus_tag="LAF92_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794177.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          136 a          118 c          125 g          153 t
ORIGIN      
        1 gacttctggt tcgctcaatg gttcttacac aaacttaggt tcacatagag gttcattctc
       61 agggcggaat tcagggggca gtacattgtt tatttatgca tctggaggta atggaggatc
      121 tgccggaggt gcatgtgcaa atacatcccg actgcaggga tatgttggtg ggacgctaat
      181 tagcgtgaat gccagcaata accctgcata tggaaaaaca gcctttatca gctttgctgt
      241 acctgcaggt acttcctatc agataacatc ctatccaaca gaaaatacat catgtggtgc
      301 cggggtattt tcagtatttg gatatcaaac ttaattgagc gttacacacg acgcaataca
      361 acctgtctta tgctcgtatt tgtaccattt acgtgaacct gctgggctct caactacctg
      421 gcagtagtgg gcatcctctg cgttgcccat tctggcaatg gcacagaagt cgaattgccc
      481 cagataacct gtggtggaaa gctgagtaac tttgagtttt ccacctattg tc
//
LOCUS       DAEVZA010000382          519 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEVZA010000382 DAEVZA010000000
VERSION     DAEVZA010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.394"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>519
                     /locus_tag="LAF92_005322"
     CDS             <1..>519
                     /locus_tag="LAF92_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI9794178.1"
                     /translation="YGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEY
                     DEAGRLKAQTDAAGRRTEYRLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGL
                     RSSREYDERGRLTAETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLA
                     FTDCSGYTTRYE"
BASE COUNT          116 a          123 c          214 g           66 t
ORIGIN      
        1 agtacggaga gagccgggta atcatcacgg acagcctgaa ccggcgggag gtgctgtaca
       61 cggaaggcga gggtggcctg aagcgggtgg tgaagaagga gcatgccgac gggagcatca
      121 cccgcagcga gtatgatgag gcggggaggc tgaaggcgca gacggatgcg gcggggcggc
      181 ggacggagta ccggctgcat atggcgtcgg gcgcggtgac agcggtgacg gggccggacg
      241 gcaggacggt gcggtatggc tataacagcc agcggcaggt gacgtcagtg acgtacccgg
      301 acgggcttcg cagcagccgg gagtatgatg agaggggaag gctgacggcg gagacctcgc
      361 gcagcggtga gacaacgcgg tacagctatg atgacccggc gagtgagctg ccgacaggga
      421 tacaggacgc gacgggcagt acaaaacaga tggcatggag ccgttacggt caactgctgg
      481 ccttcacgga ctgctcgggg tacacgacgc ggtatgagt
//
LOCUS       DAEVZA010000383          515 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEVZA010000383 DAEVZA010000000
VERSION     DAEVZA010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.395"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            43..225
                     /locus_tag="LAF92_005323"
     CDS             43..225
                     /locus_tag="LAF92_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794179.1"
                     /translation="MMARKFGKQLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSH
                     TKSELTGGKRRYRIKN"
BASE COUNT          156 a           87 c          132 g          140 t
ORIGIN      
        1 tggcctgatt taggtggtgg atcagcagta tcacagtttg caatgatggc cagaaaattt
       61 ggtaaacaac ttattgtcgc tgtaagtgaa aaggatgcca tcaaattaaa agacaatttt
      121 gatattattg ggatgttgtc atatggtaaa gaaaatttta ttgccatgag tcatacgaaa
      181 agcgagttaa ctggagggaa acgtcggtac cggattaaaa attgatataa ataaaaagca
      241 tacttattgg ctggagtttc ggtagtaaaa agcgggaaat gaaggtaata gtcagcaaca
      301 gggaatgtgg tattatcgcg gcgggtgtct gagcctttct ggttcaggca agacgcaggt
      361 accagaaatg cgaagacccc actcgttaat ccattaactc gtgaggtctg catgaagtac
      421 cttaacacta ctgattgtag cctcttcctt gcagggaggt caaagtttat gacgaaatat
      481 gcccttatcg ggttgctcgc cgtgtgcgcc acggt
//
LOCUS       DAEVZA010000384          510 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEVZA010000384 DAEVZA010000000
VERSION     DAEVZA010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.396"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>510
                     /locus_tag="LAF92_005324"
     CDS             <1..>510
                     /locus_tag="LAF92_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBI9794180.1"
                     /translation="LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGR
                     VLAVNSQTRTLTLDREITLPSSGTTLISLVDGQGSPVSVEVQSVTDGVKVKVSRVPDG
                     VAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQS
                     GTVNGVTPP"
BASE COUNT          108 a          140 c          172 g           90 t
ORIGIN      
        1 ggctgattaa aacagaactg ctggaaacgc agaccgtgga cttcagcgtg ggtgccgaag
       61 ggcttcgcca tgtaccgggc gatgtcattg aaatctgcga tgatgactat gccggtatcc
      121 gcaccggcgg gcgcgtgctg gcggtaaaca gccagacccg gacgctgacg ctcgaccgtg
      181 aaatcacgct gccatcttcc ggcaccacgc tgataagcct ggttgacggg caggggagtc
      241 cggtcagcgt ggaggttcag tccgtcaccg acggcgtgaa ggtgaaagtg agccgtgttc
      301 ctgacggcgt tgctgaatac agcgtatggg ggctgaagct gccgacgttg cgccagcgcc
      361 tgttccgctg cgtgagtatc cgtgagaacg acgacggcac gtatgccatc accgccgtgc
      421 agcatgtacc ggaaaaagag gccatcgtgg ataacggggc gcactttgac ggcgaccaga
      481 gcggcacggt aaatggtgtc acgccgccag
//
LOCUS       DAEVZA010000385          508 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEVZA010000385 DAEVZA010000000
VERSION     DAEVZA010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.397"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            1..333
                     /locus_tag="LAF92_005325"
                     /pseudo
     CDS             1..333
                     /locus_tag="LAF92_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          142 a          118 c          141 g          107 t
ORIGIN      
        1 tacacgccgg cgcgaaaaat tcacctgtac cactgcgacc atcgcggcct gccgctggcc
       61 cttatcagca cggaagggac aacagcgtgg tacgcagaat acgatgaatg gggtaaccag
      121 ctgaatgaag agaacccgca ccagctgcag cagctcatcc gcctgccggg gcagcagtat
      181 gatgaggagt ccggcctgta ttataaccgc caccgctatt atgacccgct gcgggggagg
      241 tatatcactc aggatccgat tgggctgaag gggggatgga atttttatca gtatccgttg
      301 aatccgatct caaatataga tccattagga ttagaaacac taaaatgcat taagccactg
      361 cattcaatgg gcggaactgg tgaaagaagc ggtccagata tatgggggaa tccgttctat
      421 catcaatatc tttgtgtccc agatggtaaa ggggactata cttgtggtgg ccaagaccaa
      481 cggggagaat caaaaggaga tggtctat
//
LOCUS       DAEVZA010000386          476 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEVZA010000386 DAEVZA010000000
VERSION     DAEVZA010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.398"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>476
                     /locus_tag="LAF92_005326"
     rRNA            <1..>476
                     /locus_tag="LAF92_005326"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAEVZA010000387          474 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEVZA010000387 DAEVZA010000000
VERSION     DAEVZA010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.399"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(319..>474)
                     /locus_tag="LAF92_005327"
     CDS             complement(319..>474)
                     /locus_tag="LAF92_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794181.1"
                     /translation="APHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEV
                     DAVAKAA"
BASE COUNT          155 a          110 c          101 g          108 t
ORIGIN      
        1 atgcatgttt tacgtaaagt aaattaccgc cagagaggca aaatgggcag atttgagcag
       61 gctcacaaaa tgcgattaaa tgcctgaatt acaaaagcga ggaatttgtg acgggatgca
      121 cagaacagga acactaaaaa agggcagaaa ataatctgcc cttgaggaaa taaccttagt
      181 gtttgatgaa cgtttcaggt ttctactgaa agataaaacc atttaaattc ataaaattac
      241 atttacaaac cggtcattcc ctttgcgctc atccaatgat acagggcagg aaatttctgc
      301 cctgtaacac accttttatt acgctgcttt cgctactgcg tccacttccg gacgtttcag
      361 gaaggcatag gccaaacccg ccaccagcgt accggcaata attgctacca ggtaacccaa
      421 taccggcgta atagcgccag ggatcagcag aacaaacaga ccaccgtgcg gtgc
//
LOCUS       DAEVZA010000388          464 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEVZA010000388 DAEVZA010000000
VERSION     DAEVZA010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.400"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..228)
                     /locus_tag="LAF92_005328"
     CDS             complement(<1..228)
                     /locus_tag="LAF92_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI9794182.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADM"
BASE COUNT          130 a          112 c           97 g          125 t
ORIGIN      
        1 catatcggca gtgtcgcggc caagacggtc gagcttctta accagaatga catcaccttc
       61 ctccaccttc atcctcagca aatccagccc ttcccggtct gttgaactgc cggatgcctt
      121 atcggtaaat atacggtttg ctttcacacc tgcgtctttg agtgctctga cctgaagatc
      181 aagagactgc tgactggttg agacccgagc gtaaccaaaa agtcgcataa aaatgtacct
      241 taaatcgaat atcggacaac tcatgtctat tattacaaat ttacgattta atagacatat
      301 taatgtaaca gttttacgat gtccgataat ttataacatt tcgtacggtt ggaaaaatgt
      361 tactaaatgc ccgtcaggca gggaggccga tatgcccgtt gactttctga ccactgagca
      421 gactgaaagc tatggcagat tcaccggtga accggatgag cttc
//
LOCUS       DAEVZA010000389          454 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEVZA010000389 DAEVZA010000000
VERSION     DAEVZA010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.401"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>454)
                     /locus_tag="LAF92_005329"
     rRNA            complement(<1..>454)
                     /locus_tag="LAF92_005329"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           83 a          145 c           99 g          127 t
ORIGIN      
        1 gccttctccc gaagttacgg caccattttg cctagttcct tcacccgagt tctctcaagc
       61 gccttggtat tctctacctg accacctgtg tcggtttggg gtacgatttg atgttacctg
      121 atgcttagag gcttttcctg gaagcagggc atttgttgct tcagcaccgt agtgcctcgt
      181 catcacgcct cagccttgat tttccggatt tgcctggaaa accagcctac acgcttaaac
      241 cgggacaacc gtcgcccggc caacatagcc ttctccgtcc ccccttcgca gtaacaccaa
      301 gtacaggaat attaacctgt ttcccatcga ctacgccttt cggcctcgcc ttaggggtcg
      361 actcaccctg ccccgattaa cgttggacag gaacccttgg tcttccggcg agcgggcttt
      421 tcacccgctt tatcgttact tatgtcagca ttcg
//
LOCUS       DAEVZA010000390          448 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEVZA010000390 DAEVZA010000000
VERSION     DAEVZA010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.402"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>448
                     /gene="rsxB"
                     /locus_tag="LAF92_005330"
     CDS             <1..>448
                     /gene="rsxB"
                     /locus_tag="LAF92_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBI9794183.1"
                     /translation="ILPQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIA
                     ELLNVEPQPLDGEAQEITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMS
                     DLCTGCNLCVDPCPTHCISLQPVAETPDSWKWDLNTIPVRIIPVEH"
BASE COUNT          104 a          117 c          125 g          102 t
ORIGIN      
        1 aaatcttacc gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg
       61 aagccatcag ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga
      121 tgctaaaaat tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag
      181 agataacgcc tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta
      241 aatgtattca ggcgtgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg
      301 taatgagtga tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca
      361 tctcgttgca accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc
      421 ccgtgcgtat cattcccgtg gaacacca
//
LOCUS       DAEVZA010000391          442 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEVZA010000391 DAEVZA010000000
VERSION     DAEVZA010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.403"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            159..>442
                     /locus_tag="LAF92_005331"
     CDS             159..>442
                     /locus_tag="LAF92_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI9794184.1"
                     /translation="MDIEIRHCNNIVRAHITLTADKLNIKFAPNGTGKSTLSRAISCA
                     ARDDIQGLQALMPFRLRGENPDSTGPIVIGADGIGDVMCFNEEYVSQFTF"
BASE COUNT          126 a          104 c          115 g           97 t
ORIGIN      
        1 aagtcggatc gacctaatct ttgtttctga tatgaacacc tcggagaggt gtaagcaatg
       61 gtataaacga aatgtattat gtatctgcat cacagcatgc gggcacattt tgcggaagga
      121 tatgaatccc cccggaataa ctggcaaagg acagataaat ggacattgaa atacgccact
      181 gcaataacat cgtacgagca catatcaccc tcactgccga taaactgaac attaagtttg
      241 cgccaaatgg tactggaaaa agtacgctgt cacgggccat aagctgtgcg gcgcgggacg
      301 acattcaggg actacaggca ctgatgccct tccggctgcg cggagaaaac cccgatagca
      361 ccgggcccat tgtcatcggt gctgacggga ttggggacgt gatgtgcttc aacgaggagt
      421 acgtcagtca gtttacgttc ca
//
LOCUS       DAEVZA010000392          436 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEVZA010000392 DAEVZA010000000
VERSION     DAEVZA010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.404"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..185
                     /locus_tag="LAF92_005332"
     CDS             <1..185
                     /locus_tag="LAF92_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794185.1"
                     /translation="STTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSISD
                     LLVVVMKKSTAGISIS"
     gene            252..>436
                     /locus_tag="LAF92_005333"
     CDS             252..>436
                     /locus_tag="LAF92_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBI9794186.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDT"
BASE COUNT          113 a          105 c          110 g          108 t
ORIGIN      
        1 agtccacgac ggatgctcag ggcgtatttt cctcagttat agatatgcct gccggacagg
       61 ggacgctgac actgacattc accgtatctt catcaggagc gaataactgg acaccaacaa
      121 ccagtatcag cgatctgctg gttgtggtga tgaaaaaatc cacagcaggt atcagtatca
      181 gctgaatttt ataacccaga acgggcgtca gaaatgacgc cttttttatt gcagaaaagc
      241 gagaggtaat tatgcgtaaa ctttatgccg ccattttgtc cgcagccatt tgtctggccg
      301 tatccggtgc gcctgcatgg gcatctgaac atcagtccac gctgagcgcg gggtatcttc
      361 atgcccggac caacgttccc ggcagtgatg atctgaacgg gattaacgtg aaataccgtt
      421 atgagtttac ggatac
//
LOCUS       DAEVZA010000393          433 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEVZA010000393 DAEVZA010000000
VERSION     DAEVZA010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.405"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            82..>433
                     /gene="lplA"
                     /locus_tag="LAF92_005334"
     CDS             82..>433
                     /gene="lplA"
                     /locus_tag="LAF92_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBI9794187.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAE"
BASE COUNT          104 a          115 c          125 g           89 t
ORIGIN      
        1 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
       61 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
      121 ccgtggttta acctggcggt ggaggagtgt atttttcgcc agatgcccgc cacgcagcgc
      181 gttctgtttc tctggcgcaa tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg
      241 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcacg gcgcagtagc
      301 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
      361 ccggagtacg ataaaaccat ctccacgtcg attgtgctca atgcgctgaa cgcgcttggc
      421 gtcagcgccg aag
//
LOCUS       DAEVZA010000394          430 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEVZA010000394 DAEVZA010000000
VERSION     DAEVZA010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.406"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            44..>430
                     /locus_tag="LAF92_005335"
     CDS             44..>430
                     /locus_tag="LAF92_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBI9794188.1"
                     /translation="MSETIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQ
                     VVTSPDKAYYWIQANVLKADKMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGA
                     TLGVGLAAGLVGMAADAMVEDVNYTMI"
BASE COUNT          112 a           89 c          134 g           95 t
ORIGIN      
        1 gagcacagca atcaagaagc gtaaccttga ggtgaagact cagatgagtg agaccatctg
       61 gcttgaaccc gccagcgaac gcacggtatt tctgcagatc aaaaacacgt ctgataaaga
      121 catgagtggg ctgcagggca aaattgctga tgctgtgaaa gcaaaaggat atcaggtggt
      181 gacttctccg gataaagcct actactggat tcaggcgaat gtgctgaagg ccgataagat
      241 ggatctgcgg gaatctcagg gatggctgaa ccgtggttat gaaggcgcag cagttggtgc
      301 agcgttaggt gccggtatca ccggatataa ttccagttct gccggtgcca cactcggtgt
      361 gggccttgct gccggtctgg tgggtatggc tgcagatgcg atggtggaag atgtgaacta
      421 taccatgatc
//
LOCUS       DAEVZA010000395          415 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.407, whole genome shotgun sequence.
ACCESSION   DAEVZA010000395 DAEVZA010000000
VERSION     DAEVZA010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.407"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            105..>415
                     /locus_tag="LAF92_005336"
     CDS             105..>415
                     /locus_tag="LAF92_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HBI9794189.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SL"
BASE COUNT           84 a           95 c          125 g          111 t
ORIGIN      
        1 gcgctgctgg ttgtcgatcg tcagatgagc tacatgcgtc agcagctttc cgcccgcatg
       61 ctcagtcgtt accagaacaa ctatcacttc ggagggagca cgctgtgaat gaagtttatg
      121 tgattgccgg tggtgagtgg ttgcggaata acctgaacgc cattgccgcc tttatgggaa
      181 ccaggacgtg ggattccatt gaaaaaattg cgctcacatt gtctgttctc gcggtggccg
      241 tgatgtgggt acagcggcac aacgtgatgg atttgctggg ctgggtggcc gtgtttgtgc
      301 ttatcagcct gctggttaat gtccgcacat cggtgcagat tattgataac agtgacctgg
      361 tcaaagttca ccgggtggat aatgtaccgg tcggactggc gatgccactt tcact
//
LOCUS       DAEVZA010000396          414 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.408, whole genome shotgun sequence.
ACCESSION   DAEVZA010000396 DAEVZA010000000
VERSION     DAEVZA010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.408"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(262..>414)
                     /locus_tag="LAF92_005337"
     CDS             complement(262..>414)
                     /locus_tag="LAF92_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794190.1"
                     /translation="GAKIQTSTETGNQHKYQTRVVSNANKVNLKFEEAKPVLEDQLAK
                     SIANIL"
BASE COUNT          115 a           87 c           95 g          117 t
ORIGIN      
        1 aaaactcatg atgatattcc gaagaaaaaa agtcagtcgg actgattatt aatgatgaaa
       61 agattaataa ccgttaaatt ccggcttcgc cggcaacact tcacggtcgc acaaagaatt
      121 tgcacaaccg ctcagtgttt tcatatttat ttcggaaacg ccggggagaa ataaaattta
      181 ttttatttct ttccggggtt ggacaacgag caaagcgagg cgtcagtcag gaggccggtc
      241 aggccagcct ccggaagata atcagagaat atttgcgatt gatttggcca gctggtcttc
      301 gagaacaggc ttcgcctctt caaatttcag gttaaccttg ttcgcatttg agaccacacg
      361 ggtctggtat ttatgctggt tacctgtttc agtactggtc tgaattttcg cacc
//
LOCUS       DAEVZA010000397          402 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.409, whole genome shotgun sequence.
ACCESSION   DAEVZA010000397 DAEVZA010000000
VERSION     DAEVZA010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.409"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>402
                     /locus_tag="LAF92_005338"
     CDS             <1..>402
                     /locus_tag="LAF92_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794191.1"
                     /translation="QVLARWDTPKVVKGVSFLLRLTVAADDGRERLVSTARTTETTYR
                     FTQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAV
                     YDPTVQFEFWFSEKRIADIRQVETSARYLGTA"
BASE COUNT           75 a          113 c          137 g           77 t
ORIGIN      
        1 ccaggtgctg gcccgctggg acacgccgaa ggtggtgaag ggcgtgagct tcctgcttcg
       61 cctgaccgtg gcagcggatg acggccgtga gcggctggtc agcacggccc ggacgacgga
      121 aaccacttac cgcttcacac aactggctct ggggaactac aggctgacag tccgggcagt
      181 aaatgcgtgg gggcagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc
      241 agcgccgtcg cggattgagc tgacgccggg ctattttcag ataaccgcca cgccgcatct
      301 tgcggtttat gacccgacgg tacagtttga gttctggttc tcggaaaaac ggattgcgga
      361 tatcaggcag gttgaaacca gcgcgcgtta tcttggtacg gc
//
LOCUS       DAEVZA010000398          384 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.410, whole genome shotgun sequence.
ACCESSION   DAEVZA010000398 DAEVZA010000000
VERSION     DAEVZA010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.410"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>384
                     /locus_tag="LAF92_005339"
     CDS             <1..>384
                     /locus_tag="LAF92_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794192.1"
                     /translation="KAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLT
                     SATGPDGLEMRRKYDEYGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTW
                     SRYGQLLSFTDCSGYVTRYDHDRFGQ"
BASE COUNT           93 a          117 c          106 g           68 t
ORIGIN      
        1 aaggcacaga cggatgccgc aggccggaca acagagtaca gcccggatgt ggtgacgggc
       61 ctcatcacgc gcatcaccac gccggatggc agggcatcgg cgttttacta taaccaccac
      121 agccagttaa cgtcagccac cgggccagac gggctggaaa tgcgccgcaa atatgatgag
      181 tacggacgtc tgattcagga aaccgcccct gacggcgata tcacccgcta ccgttatgat
      241 aatccacaca gtgacctccc ctgcgcaacg gacgatgcca ccggcagccg gaaaaccatg
      301 acgtggagcc gttacggtca gttgctgagc ttcactgact gttccggtta tgtaacccgc
      361 tatgaccatg accgttttgg tcag
//
LOCUS       DAEVZA010000399          382 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEVZA010000399 DAEVZA010000000
VERSION     DAEVZA010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.411"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>382
                     /locus_tag="LAF92_005340"
     rRNA            <1..>382
                     /locus_tag="LAF92_005340"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           83 c          128 g           84 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc ta
//
LOCUS       DAEVZA010000400          377 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEVZA010000400 DAEVZA010000000
VERSION     DAEVZA010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.412"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>377)
                     /locus_tag="LAF92_005341"
     CDS             complement(<1..>377)
                     /locus_tag="LAF92_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI9794193.1"
                     /translation="AVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTAT
                     ISPDGKRSTIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDR
                     LTEQRGFDGRTQRYHYDLTGKLT"
BASE COUNT           59 a          119 c          106 g           93 t
ORIGIN      
        1 gcgtgagttt tccggtcagg tcatagtggt aacgttgcgt ccggccgtca aaaccgcgct
       61 gttcagtcag cctgtccacc gggtcatacc ggaacgtgga ctggctgccg ttctcgttgg
      121 tcagcacggt gatgcgcccg gcagcgtcat accccatgct gcgcgtaaga ccgccttccg
      181 tcacactcac cggacgcccg cgtttatcat attcgatggt gctgcgttta ccgtccggtg
      241 atatcgttgc tgtcaggtcg cctgcggcgc tgtactcata acggatttca cgcccctgcg
      301 catccttctg actgaccagt tgcccacgcg ggttataact gctgtaagtg ctgatgcctt
      361 cctcccggtg aacggcg
//
LOCUS       DAEVZA010000401          370 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEVZA010000401 DAEVZA010000000
VERSION     DAEVZA010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.413"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>370
                     /locus_tag="LAF92_005342"
     CDS             <1..>370
                     /locus_tag="LAF92_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794194.1"
                     /translation="LGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTGAS
                     AVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIG
                     SHGHTITVNAAGNAENTVKNI"
BASE COUNT          101 a          100 c           97 g           72 t
ORIGIN      
        1 ttggggacga aaaccacatc gtcgtttgat tacggcacta aatccacgaa taacaccggg
       61 gcgcacacgc acagtgtgag cggtacagcc gcaagtgccg gaaaccatac tcatagtgtc
      121 acaggcgcat cagcagtcag ccagtggtca caaaatgggt cagtacataa ggtagtgtct
      181 gcggccagtg tgaatacaag tgctgcagga gcgcacactc atagtgtcag cggcacagct
      241 gcatctgcag gtgctcacgc acatactgtc ggtattggtg ctcatacgca ctctgttgcg
      301 attggctcac atggacacac catcaccgtt aacgctgctg gtaacgcgga aaacaccgtc
      361 aaaaacatcg
//
LOCUS       DAEVZA010000402          362 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.414, whole genome shotgun sequence.
ACCESSION   DAEVZA010000402 DAEVZA010000000
VERSION     DAEVZA010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.414"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>362
                     /gene="traV"
                     /locus_tag="LAF92_005343"
     CDS             <1..>362
                     /gene="traV"
                     /locus_tag="LAF92_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HBI9794195.1"
                     /translation="LLLSGCAGTSTEFECNATTSDTCMTMEQANEKAKKLEQSSEAKP
                     VAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPRPLFTAAREVKTIV
                     PVSSVAPVTPPRPLRTGE"
BASE COUNT           87 a          110 c          102 g           63 t
ORIGIN      
        1 ttattacttt ccggctgtgc cggaaccagt acggaatttg agtgtaacgc caccacatcc
       61 gatacctgta tgacgatgga gcaggccaat gagaaggcca aaaaactgga gcagtcctca
      121 gaagcaaagc cggttgcggc atcactgccg cgcctggctg aagggaactt ccggacaatg
      181 ccggtgcaga ccgtcaccgc gaccactccg tccggcagca gacctgcggt gacagcacat
      241 ccggagcaga aactgctggc accacgcccg ctgtttaccg ctgcccggga agtgaaaacg
      301 attgttccgg tcagttcagt tgcgccggta acacctcccc gtccgctaag aacgggtgag
      361 ca
//
LOCUS       DAEVZA010000403          355 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEVZA010000403 DAEVZA010000000
VERSION     DAEVZA010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.415"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>355
                     /locus_tag="LAF92_005344"
     CDS             <1..>355
                     /locus_tag="LAF92_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI9794196.1"
                     /translation="GLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGS
                     RNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETG
                     FDGRTQRYHHDLTGKL"
BASE COUNT           93 a          107 c          102 g           53 t
ORIGIN      
        1 aagggctgag tcagtaccgc gcatacgaca gccgtggaca gttaattgcc gtgaaagaca
       61 cgcagggcca tgaaacgcgg tatgaataca acgccgccgg tgacctgacc accgtcattg
      121 ccccggacgg cagcagaaac gggacacagt acgatgcgtg gggaaaagcc atctgtacca
      181 cgcagggcgg tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga
      241 ccagtgaaaa cggcagccac accaccttcc gttacgatgt actcgaccgg ctgatacagg
      301 aaaccggctt tgacggccgc acacagcgtt atcaccacga cctgaccggc aaact
//
LOCUS       DAEVZA010000404          355 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEVZA010000404 DAEVZA010000000
VERSION     DAEVZA010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.416"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>355)
                     /locus_tag="LAF92_005345"
     CDS             complement(<1..>355)
                     /locus_tag="LAF92_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI9794197.1"
                     /translation="QEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQMDGWRNNAET
                     ARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENH
                     FAVIPADRTWRPQPLL"
BASE COUNT           46 a          128 c           94 g           87 t
ORIGIN      
        1 tcagcaacgg ctgtggtcgc catgttctgt ctgccgggat caccgcaaaa tggttctcca
       61 gcgccgtccc cgctccctgc cgtcctggca ccgcctgtgg ctgttcgccg tgcagttcac
      121 ttgccaccac ctgccattcc cggttcaggt tcgcctgcgg atgccccgtc agcacaattc
      181 gtcgtcccgg ccatatctcc ggcgagcgac tcattccccg cgcggtttct gcattgtttc
      241 gccagccgtc catctgccag cgggcaaagt tctgcccgtg ggcccccttg aaacgtccgg
      301 ggtagtcata cacttcatac tgtgtgcgct ggtaatcctg gtgctggcct tcctg
//
LOCUS       DAEVZA010000405          337 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEVZA010000405 DAEVZA010000000
VERSION     DAEVZA010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.417"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>337)
                     /locus_tag="LAF92_005346"
     CDS             complement(<1..>337)
                     /locus_tag="LAF92_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI9794198.1"
                     /translation="EEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLG
                     REGYTKVQNASYQVATYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLY
                     DLSERLRLRG"
BASE COUNT           79 a           94 c           91 g           73 t
ORIGIN      
        1 agccgcgcag acgcagacgt tcagagaggt catacagggt gtatcccgga tcttcaccat
       61 ctttcagttt gaagcaaacc gccgggatgc cttcgtccgg gcgaccagta cagatgaact
      121 catacggccc cagtttggcg atttcatccg ccagataggt agcgacctga taagaggcgt
      181 tctgtacttt ggtatagcct tcacgaccga ggcgcaggaa ttcatagtac tgtgcaatca
      241 cctgacctgc cgggcgggag aagttgatgg cgaaagtacc aatctgaccc cccaggtagt
      301 caacgttgaa caccagttcc tgcggcagcg cttcttc
//
LOCUS       DAEVZA010000406          323 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.418, whole genome shotgun sequence.
ACCESSION   DAEVZA010000406 DAEVZA010000000
VERSION     DAEVZA010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.418"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..231
                     /locus_tag="LAF92_005347"
     CDS             <1..231
                     /locus_tag="LAF92_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HBI9794199.1"
                     /translation="IGQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNV
                     QTGQNNLQSAKSVAQLTSALSELAKQFWEKRG"
BASE COUNT          101 a           71 c           75 g           76 t
ORIGIN      
        1 attggccaac gcgttaacga aattaccgac atgaacgcgc aaatagcgac tgcggtcgag
       61 cagcagggag cagtaagtga agacataaac cgcagtatta tcaatatacg cgatgctgct
      121 gataccaatg tacagaccgg gcagaataat ttgcaaagtg cgaaatctgt cgctcagtta
      181 actagcgctc tgagcgaact ggcaaaacag ttttgggaaa aacgaggata acgcttttca
      241 gtatctcgac agggataact tactttacca tcggttatcc cttttagccg cacaaatttt
      301 agccatggct tttcaggaag tca
//
LOCUS       DAEVZA010000407          318 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.419, whole genome shotgun sequence.
ACCESSION   DAEVZA010000407 DAEVZA010000000
VERSION     DAEVZA010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.419"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>318)
                     /locus_tag="LAF92_005348"
     CDS             complement(<1..>318)
                     /locus_tag="LAF92_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794200.1"
                     /translation="KDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKEL
                     LEQVKREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSL
                     DLN"
BASE COUNT           86 a           67 c           47 g          118 t
ORIGIN      
        1 tagtttaaat ctaacgaacc atcagtttta tcaagcttag ttttcagcct tttaacagcc
       61 tccaaggaag catcaagaac tggtgtctga ttcttcaaag cttctgaaaa acttttttgt
      121 gttgattttc gagcttgttg ttgagcagat tttcttaact ctttttcacg cttaacttgt
      181 tctaaaagct ctttacggtc atcagaacgt gaaccaaaaa atctatcagc aagttcagtt
      241 aacaaattag atataatagt tttcagttct cttgcagcct ggtttcttat gaatccctct
      301 ctccccgact tatctttc
//
LOCUS       DAEVZA010000408          314 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.420, whole genome shotgun sequence.
ACCESSION   DAEVZA010000408 DAEVZA010000000
VERSION     DAEVZA010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.420"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>314
                     /gene="tuf"
                     /locus_tag="LAF92_005349"
     CDS             <1..>314
                     /gene="tuf"
                     /locus_tag="LAF92_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI9794201.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAEVZA010000409          306 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.421, whole genome shotgun sequence.
ACCESSION   DAEVZA010000409 DAEVZA010000000
VERSION     DAEVZA010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.421"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
BASE COUNT           69 a           79 c           82 g           76 t
ORIGIN      
        1 ctcgcgctaa ctgcggcatt ctgcggcgca aatcagcata atcatggcct gctggtcagt
       61 tgggctttgc agggtgaagg gatggcctgt gcaccctggt acgttcgtca gcggctgcgc
      121 cgcgcttcgg ggcgatgtgg ctcctgtgct gccctggtat tttcgttcgc cccttccatc
      181 cgcttacgcg gattaaaaaa cgtgataaca atcacttatt gagtaaaaaa acgggagaac
      241 acttgcaaat acgacctaaa agtcgtatga ttgtttcatc gaaaggggaa tggcccctct
      301 cacaat
//
LOCUS       DAEVZA010000410          302 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.422, whole genome shotgun sequence.
ACCESSION   DAEVZA010000410 DAEVZA010000000
VERSION     DAEVZA010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.422"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            76..297
                     /gene="traR"
                     /locus_tag="LAF92_005350"
     CDS             76..297
                     /gene="traR"
                     /locus_tag="LAF92_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HBI9794202.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPV
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
BASE COUNT           92 a           57 c           86 g           67 t
ORIGIN      
        1 gcctgtaaaa cgagtgccct gtgcgtgaaa agggatgggc ggtagcgtgt cagaacatgt
       61 cgggaggaat attccgtgag tgatgaagcc gatgaagcat attcagtgac agaacaactg
      121 accatgacag gaataaaccg gatacgccag aaaataaatg ctcatggtat tcctgtttat
      181 ctctgtgaag catgcggaaa tcctgttccg gaagcccggc ggaaaatatt tcccggtgtg
      241 acgttgtgcg ttgaatgtca ggcgtatcag gaaagacaga gaaaacatta tgcataagtc
      301 ag
//
LOCUS       DAEVZA010000411          295 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.423, whole genome shotgun sequence.
ACCESSION   DAEVZA010000411 DAEVZA010000000
VERSION     DAEVZA010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.423"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>295
                     /locus_tag="LAF92_005351"
     rRNA            <1..>295
                     /locus_tag="LAF92_005351"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           75 a           73 c           83 g           64 t
ORIGIN      
        1 cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
       61 cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc cacccgagac
      121 tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg gaaagacccc
      181 gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg ataggtggga
      241 ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca ccctt
//
LOCUS       DAEVZA010000412          294 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.424, whole genome shotgun sequence.
ACCESSION   DAEVZA010000412 DAEVZA010000000
VERSION     DAEVZA010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.424"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            158..232
                     /gene="tap"
                     /locus_tag="LAF92_005352"
     CDS             158..232
                     /gene="tap"
                     /locus_tag="LAF92_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBI9794203.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            225..>294
                     /locus_tag="LAF92_005353"
     CDS             225..>294
                     /locus_tag="LAF92_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HBI9794204.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRE"
BASE COUNT           84 a           68 c           73 g           69 t
ORIGIN      
        1 cgtaaggtgg cagggaactg gttctgatgt ggatttacag gagccagaaa agcaaaaacc
       61 ccgataatct tcaccaggtt tggcgactaa gagaagatta ccggggccca cttaaaccgt
      121 atagccaaca attcagctat gcggggagta tagttatatg cccggaaaag ttcaagactt
      181 ctttctgtgc tcactccttc tgcgcattgt aagcgcagga tggtgtgact gatcttcaac
      241 aaacgtatta ccgccaggta aagaacccga atccggtgtt tacaccccgt gaag
//
LOCUS       DAEVZA010000413          294 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.425, whole genome shotgun sequence.
ACCESSION   DAEVZA010000413 DAEVZA010000000
VERSION     DAEVZA010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.425"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            43..>294
                     /locus_tag="LAF92_005354"
     CDS             43..>294
                     /locus_tag="LAF92_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBI9794205.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKV"
BASE COUNT           67 a           74 c           80 g           73 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtc
//
LOCUS       DAEVZA010000414          283 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.426, whole genome shotgun sequence.
ACCESSION   DAEVZA010000414 DAEVZA010000000
VERSION     DAEVZA010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.426"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>283)
                     /locus_tag="LAF92_005355"
     CDS             complement(<1..>283)
                     /locus_tag="LAF92_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794206.1"
                     /translation="RTQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQ
                     QAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPL"
BASE COUNT           44 a           91 c           94 g           54 t
ORIGIN      
        1 ccagcggcgc ggcaggtaaa ttctccgggt attccgggtc gtgcgtcagc cacacggcag
       61 acagacggat gccgttgtcc cggccatatt cggtgtaacc cggcagggta tcaggaaaag
      121 cggacggttc cgtcccgccg gaaatggcct gctgccgggc ttcttctgcc cgctgcgcct
      181 gcgtggtcag taccagccgg aagtgacgcc cggcaccatc cgtcacgccg gtgatttcgc
      241 cgctgaattc accggcggct tcgcggtgga acgtctgtgt gcg
//
LOCUS       DAEVZA010000415          261 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.427, whole genome shotgun sequence.
ACCESSION   DAEVZA010000415 DAEVZA010000000
VERSION     DAEVZA010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.427"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>261
                     /gene="hexR"
                     /locus_tag="LAF92_005356"
                     /gene_synonym="yebK"
     CDS             <1..>261
                     /gene="hexR"
                     /locus_tag="LAF92_005356"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBI9794207.1"
                     /translation="NEDDSVESYTGKIFESAMATLDHVRHSLDKSAINRAVDLLTQAK
                     KIAFFGLGSSAAVAHDAMNKFFRFNVPVVYSDDIVLQRMSCM"
BASE COUNT           68 a           62 c           59 g           72 t
ORIGIN      
        1 caatgaagat gacagcgttg aatcatacac agggaaaata tttgagtccg caatggcaac
       61 gcttgatcat gtccgtcatt cactggataa atctgccatc aaccgcgccg tcgacttgct
      121 cactcaggca aaaaaaatcg cctttttcgg attaggctct tcagccgccg ttgcccacga
      181 tgcgatgaat aagttctttc gttttaatgt tccggtggtg tactccgatg atatcgtgct
      241 gcaacgcatg agttgtatga a
//
LOCUS       DAEVZA010000416          260 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.428, whole genome shotgun sequence.
ACCESSION   DAEVZA010000416 DAEVZA010000000
VERSION     DAEVZA010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.428"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..213
                     /locus_tag="LAF92_005357"
     CDS             <1..213
                     /locus_tag="LAF92_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HBI9794208.1"
                     /translation="FLLFLEQIQVLNLETREMVIERVLALDTAEFDLEDLKWVILMVL
                     FNIPGCENAYQQMEELLFEVNEGMLH"
BASE COUNT           71 a           48 c           64 g           77 t
ORIGIN      
        1 tttctgcttt tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata
       61 gagcgagtgc tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc
      121 ctgatggtgt tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta
      181 ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt
      241 tatggcgaaa tcagcactgt
//
LOCUS       DAEVZA010000417          259 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.429, whole genome shotgun sequence.
ACCESSION   DAEVZA010000417 DAEVZA010000000
VERSION     DAEVZA010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.429"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>259
                     /locus_tag="LAF92_005358"
     CDS             <1..>259
                     /locus_tag="LAF92_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBI9794209.1"
                     /translation="KPALGCTEPISLALAAAVAAAELEGPVERVEAWVSPNLMKNGLG
                     VTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQAIADA"
BASE COUNT           51 a           62 c           98 g           48 t
ORIGIN      
        1 taaaaccggc gctgggatgt actgaaccga tttcactggc gctggcggcg gcggttgctg
       61 cggcagaact ggaaggtccg gttgaacgtg tagaagcctg ggtttcgcca aatctgatga
      121 agaacggtct gggcgtcacc gttcccggca cgggaatggt ggggctgccg attgcggcgg
      181 cgctgggggc gttaggtgga aatgccaacg ccgggctgga agtgctgaaa gacgcaactg
      241 cgcaggcaat tgccgatgc
//
LOCUS       DAEVZA010000418          257 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.430, whole genome shotgun sequence.
ACCESSION   DAEVZA010000418 DAEVZA010000000
VERSION     DAEVZA010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.430"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            complement(<1..>257)
                     /locus_tag="LAF92_005359"
     CDS             complement(<1..>257)
                     /locus_tag="LAF92_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBI9794210.1"
                     /translation="RDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEATGLYQVPLSS
                     AQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRER"
BASE COUNT           67 a           80 c           56 g           54 t
ORIGIN      
        1 tacctctctc gtttactcag ttgttcagga atatggtgca gcagctcgcc gtcaccacag
       61 taaatggcgg catgattcgg caccgatgaa ccaaaacagc acagcagcac atcgcccggt
      121 tgtgctgatg acaacggcac ctgatacagc cctgtggcct ccagattatc cagatagaga
      181 ttctgaccgt tacgccacca gtcatcctca cgatgaaaat ccggcatctc aatccccgcc
      241 agatgataag catcccg
//
LOCUS       DAEVZA010000419          255 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.431, whole genome shotgun sequence.
ACCESSION   DAEVZA010000419 DAEVZA010000000
VERSION     DAEVZA010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.431"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>255
                     /locus_tag="LAF92_005360"
     CDS             <1..>255
                     /locus_tag="LAF92_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HBI9794211.1"
                     /translation="DDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLL
                     LRLLMARVAEQYGKNEMALLLLEELDTAAQGITLTQWEPEL"
BASE COUNT           62 a           66 c           90 g           37 t
ORIGIN      
        1 agacgacgcc acaaatgacg tactggcgct ggagccggaa gcactggaga tggcagacag
       61 tcagggcgtg gaagcggcgc tgggctggat acagacccgt ccgggcatca ccactgcacg
      121 acagcgcctg ttattgcgac tgctgatggc gcgggtggcg gaacagtacg gtaaaaacga
      181 gatggcgctc ctgctgctgg aagaactgga tactgccgca cagggaataa cgctgacgca
      241 gtgggagccg gaact
//
LOCUS       DAEVZA010000420          246 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.432, whole genome shotgun sequence.
ACCESSION   DAEVZA010000420 DAEVZA010000000
VERSION     DAEVZA010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.432"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
BASE COUNT           74 a           57 c           39 g           76 t
ORIGIN      
        1 accaaaaatc cttctattag accaataata tcgccctgca ttccatgaac gtctttcttc
       61 tatctggaag aaatcattat ctactcgacc ataaggtaat actctcaagc tatccctaaa
      121 aatcattagt cctgcatact ttttggcctt aagatcaaag tgggaatgtt cacgttcagt
      181 atgtgatgta ttttgtgaaa ggaattcaaa cgttcctatc tgaagctcaa atgggccgac
      241 ccctgc
//
LOCUS       DAEVZA010000421          245 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.433, whole genome shotgun sequence.
ACCESSION   DAEVZA010000421 DAEVZA010000000
VERSION     DAEVZA010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.433"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>245
                     /gene="finO"
                     /locus_tag="LAF92_005361"
     CDS             <1..>245
                     /gene="finO"
                     /locus_tag="LAF92_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBI9794212.1"
                     /translation="RPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLAEKAAR
                     EAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPG"
BASE COUNT           74 a           65 c           75 g           31 t
ORIGIN      
        1 cgaccggtac tgacactgaa gcggaaaacg gaaggggaga cgcctgtccg caggcggaaa
       61 accatcatca atgtcaccac gccaccaaag tggaaggtga aaaagcagaa gctggccgag
      121 aaagcagccc gggaagcaga gctggcggca aaaaaagcgc aggccagaca ggcactgtcc
      181 atttatctga atctgcccac gctggatgag gccgtgaata ccctgaaacc ctggtggccg
      241 ggatt
//
LOCUS       DAEVZA010000422          243 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.434, whole genome shotgun sequence.
ACCESSION   DAEVZA010000422 DAEVZA010000000
VERSION     DAEVZA010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.434"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>243
                     /locus_tag="LAF92_005362"
     CDS             <1..>243
                     /locus_tag="LAF92_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI9794213.1"
                     /translation="GGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
                     CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGL"
BASE COUNT           63 a           47 c           73 g           60 t
ORIGIN      
        1 ggaggccagt actgttcagc agattatcag gcgcaactga agcggcataa tctgcgtgga
       61 agtatgagcg caaaaggttg ctgctacgat aatgcctgcg tggaaagctt ctttcattcg
      121 ctgaaagtgg aatgtatcca tggagaacac tttatcagcc gggaaataat gcgggcaacg
      181 gtgtttaatt atatcgaatg tgattacaat cggtggcggc ggcacagttg gtgtggcggc
      241 ctc
//
LOCUS       DAEVZA010000423          238 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.436, whole genome shotgun sequence.
ACCESSION   DAEVZA010000423 DAEVZA010000000
VERSION     DAEVZA010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.436"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>238
                     /locus_tag="LAF92_005363"
     CDS             <1..>238
                     /locus_tag="LAF92_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794214.1"
                     /translation="SETVLGTEVKYDTPITRTITSANIDRLRFTFGVQALVETTSKGD
                     RNPSEVRLLVQIQRNGGWVTEKDITIKGKTTSQYL"
BASE COUNT           63 a           65 c           68 g           42 t
ORIGIN      
        1 tccgagacgg tgctgggtac ggaagtgaaa tacgacacgc cgattacccg gaccatcacg
       61 tcggcaaaca tcgaccgtct gcgctttacc ttcggtgtgc aggcactggt ggaaaccacc
      121 tcaaaggggg accggaatcc gtcggaagtc cgcctgctgg ttcagataca gcgtaacggt
      181 ggctgggtga cggaaaaaga catcaccatt aaaggcaaaa ccacctcaca gtatctgg
//
LOCUS       DAEVZA010000424          221 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.denovo.437, whole genome shotgun sequence.
ACCESSION   DAEVZA010000424 DAEVZA010000000
VERSION     DAEVZA010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.denovo.437"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..>221
                     /locus_tag="LAF92_005364"
     CDS             <1..>221
                     /locus_tag="LAF92_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI9794215.1"
                     /translation="RSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNS
                     PQGPWWLLGWCERVPEADEVLPAPLPPYR"
BASE COUNT           32 a           65 c           87 g           37 t
ORIGIN      
        1 gccgcagcga gtcactgtgg ctggtgcgcg gcggcgtggc gaaactggat gaaggtcacc
       61 ggctggccgc actctggcag gcgctgccgg aagaactccg cttaagtccg catcgttatc
      121 tggcgacaaa cagtccgcag gggccgtggt ggctgctcgg ctggtgtgag cgggtgccgg
      181 aagcggatga ggtgctgcct gcgccgctgc cgccgtaccg g
//
LOCUS       DAEVZA010000425         2939 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.275, whole genome shotgun sequence.
ACCESSION   DAEVZA010000425 DAEVZA010000000
VERSION     DAEVZA010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.275"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..192
                     /locus_tag="LAF92_005365"
     CDS             <1..192
                     /locus_tag="LAF92_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HBI9794216.1"
                     /translation="GARTRATAHERTPQQERQKAAREAERGREAWTLGQGMKKPVAGC
                     YGRLTRWKGGGDVVYMALL"
     gene            complement(224..874)
                     /gene="tetR(A)"
                     /locus_tag="LAF92_005366"
     CDS             complement(224..874)
                     /gene="tetR(A)"
                     /locus_tag="LAF92_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBI9794217.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            980..2179
                     /gene="tet(A)"
                     /locus_tag="LAF92_005367"
     CDS             980..2179
                     /gene="tet(A)"
                     /locus_tag="LAF92_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HBI9794218.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2211..2909)
                     /locus_tag="LAF92_005368"
     CDS             complement(2211..2909)
                     /locus_tag="LAF92_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBI9794219.1"
                     /translation="MRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFI
                     SAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTR
                     KWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRS
                     NRTPRARIACRKSP"
BASE COUNT          540 a          928 c          913 g          558 t
ORIGIN      
        1 ggcgcgcgga cccgcgccac ggcccacgag cggacgccgc agcaggagcg ccagaaggcc
       61 gccagagagg ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat gaaaaagccc
      121 gtagcgggct gctacgggcg tctgacgcgg tggaaagggg gaggggatgt tgtctacatg
      181 gctctgctgt agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg tcaccctttc
      241 tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg acaatcaccg
      301 cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc
      361 gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt
      421 cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc atcagcgcat
      481 tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg
      541 tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg
      601 cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt
      661 cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc agtgcgtcga
      721 gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt
      781 ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg tccagggcgg
      841 cacggatcac tgtattcggc tgcaactttg tcatgcttga cactttatca ctgataaaca
      901 taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg accagcggag
      961 gctggtccgg aggccagacg tgaaacccaa cagacccctg atcgtaattc tgagcactgt
     1021 cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga
     1081 tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt
     1141 gatgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc
     1201 ggtcttgctc gtctcgctgg ccggcgctgc tgtcgactac gccatcatgg cgacggcgcc
     1261 tttcctttgg gttctctata tcgggcggat cgtggccggc atcaccgggg cgactggggc
     1321 ggtagccggc gcttatattg ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg
     1381 cttcatgagc gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat
     1441 gggcggtttc tccccccacg ctccgttctt cgccgcggca gccttgaacg gcctcaattt
     1501 cctgacgggc tgtttccttt tgccggagtc gcacaaaggc gaacgccggc cgttacgccg
     1561 ggaggctctc aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc
     1621 cctgatggcg gtcttcttca tcatgcaact tgtcggacag gtgccggccg cgctttgggt
     1681 cattttcggc gaggatcgct ttcactggga cgcgaccacg atcggcattt cgcttgccgc
     1741 atttggcatt ctgcattcac tcgcccaggc aatgatcacc ggccctgtag ccgcccggct
     1801 cggcgaaagg cgggcactca tgctcggaat gattgccgac ggcacaggct acatcctgct
     1861 tgccttcgcg acacggggat ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg
     1921 catcggaatg ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg
     1981 gcagctgcaa ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg gacccctcct
     2041 cttcacggcg atctatgcgg cttctataac aacgtggaac gggtgggcat ggattgcagg
     2101 cgctgccctc tacttgctct gcctgccggc gctgcgtcgc gggctttgga gcggcgcagg
     2161 gcaacgagcc gatcgctgat cgtggaaacg ataggcctat gccatgcggg tcaaggcgac
     2221 ttccggcaag ctatacgcgc cctaggagtg cggttggaac gttggcccag ccagatactc
     2281 ccgatcacga gcaggacgcc gatgatttga agcgcactca gcgtctgatc caagaacaac
     2341 catcctagca acacggcggt ccccgggctg agaaagccca gtaaggaaac aactgtaggt
     2401 tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc gatcaggccg
     2461 agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg atcaaacact
     2521 aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt aaaggtgagc
     2581 agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc
     2641 cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt caacaccaac
     2701 agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta
     2761 cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc taccgtcgcc
     2821 gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa tagcgaaata
     2881 ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat ctgtcggac
//
LOCUS       DAEVZA010000426         2093 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.297, whole genome shotgun sequence.
ACCESSION   DAEVZA010000426 DAEVZA010000000
VERSION     DAEVZA010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.297"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..483
                     /locus_tag="LAF92_005369"
     CDS             <1..483
                     /locus_tag="LAF92_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HBI9794220.1"
                     /translation="SQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(665..841)
                     /locus_tag="LAF92_005370"
     CDS             complement(665..841)
                     /locus_tag="LAF92_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBI9794221.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            1171..1986
                     /gene="sul2"
                     /locus_tag="LAF92_005371"
     CDS             1171..1986
                     /gene="sul2"
                     /locus_tag="LAF92_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBI9794222.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          477 a          552 c          557 g          507 t
ORIGIN      
        1 agccagttac tgctgaaatt acaacagcat tatcactatc ttgttgagca gattaaagat
       61 ctggaatctc agttgaaacg aaagttggac gaagatgagg ttggacagcg cttgctgagt
      121 attccctgcg ttggaacgct gactgccagt actatttcaa ctgagattgg cgacgggaag
      181 cagtacgcca gcagccgtga ctttgcggcg gcaacagggc tggtaccccg acagtacagc
      241 acgggaggtc ggacgacatt gttagggatt agcaagcggg gcaacaaaaa gatccgaact
      301 ttgttggttc agtgtgccag ggtattcata caaaaactgg aacaccagtc tggcaagttg
      361 gccgactggg tcagggagtt gttgtgtcgg aaaagcaact ttgtcgtcac ctgtgctctg
      421 gcaaacaagc tggccagaat agcctgggca ctgacggcgc gacagcaaac ttacgaagca
      481 taaaggcaga aatacaccag tttaaacaat cattcatctg gttttgcgaa tactgatatt
      541 gatgatacta acggcccacc ggcctgttga ggaacctgta aaacggaaag gctcattgaa
      601 gccgtatatt ttctggaggt tcatcaggcg cggaactcat cgaggcgcgg gaataaaatc
      661 ccattcagac gccggataga ttcaagcaag ccaacttgtc gtcaaaatcg gtgttgcaaa
      721 aacgggagtg accatagatt ccgttttctg aggcgacccc cacatagaga ccttcccggt
      781 cattcacttt gtagagtttg tccctgggct tgagattgcg cagcttggta tcggtcagca
      841 tgtaccttgt ccttcttcgt ctccgatacc cactctattg taaacaagac atttttatct
      901 tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat accatgctca
      961 gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac agctccatag
     1021 gccgctttcc tggctttgct tccagatgta tgctattctg ctcctgcagc taatggatca
     1081 ccgcaaacag gttactcgcc tggggattcc ctttcgaccc gagcatccgt atgagactca
     1141 tgctcgatta ttattattat agaagccccc atgaataaat cgctcatcat tttcggcatc
     1201 gtcaacataa cctcggacag tttctccgat ggaggccggt atctggcgcc agacgcagcc
     1261 attgcgcagg cgcgtaagct gatggccgag ggggcagatg tgatcgacct cggtccggca
     1321 tccagcaatc ccgacgccgc gcctgtttcg tccgacacag aaatcgcgcg tatcgcgccg
     1381 gtgctggacg cgctcaaggc agatggcatt cccgtctcgc tcgacagtta tcaacccgcg
     1441 acgcaagcct atgccttgtc gcgtggtgtg gcctatctca atgatattcg cggttttcca
     1501 gacgctgcgt tctatccgca attggcgaaa tcatctgcca aactcgtcgt tatgcattcg
     1561 gtgcaagacg ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat ggatcacatt
     1621 gcggcgttct ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa acgcaaccgc
     1681 cttgtccttg atcccggcat ggggtttttt ctgggggctg ctcccgaaac ctcgctctcg
     1741 gtgctggcgc ggttcgatga attgcggctg cgcttcgatt tgccggtgct tctgtctgtt
     1801 tcgcgcaaat cctttctgcg cgcgctcaca ggccgtggtc cgggggatgt cggggccgcg
     1861 acactcgctg cagagcttgc cgccgccgca ggtggagctg acttcatccg cacacacgag
     1921 ccgcgcccct tgcgcgacgg gctggcggta ttggcggcgc tgaaagaaac cgcaagaatt
     1981 cgttaactgc acattcggga tatttctcta tattcgcggt tcagcaggca tgtccccttt
     2041 gagggcgacc cgacgacagg ataatcgacc ttatggtgcg caaatatttc ggc
//
LOCUS       DAEVZA010000427         1794 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.311, whole genome shotgun sequence.
ACCESSION   DAEVZA010000427 DAEVZA010000000
VERSION     DAEVZA010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.311"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            <1..165
                     /locus_tag="LAF92_005372"
     CDS             <1..165
                     /locus_tag="LAF92_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="in conjunction with FrdC acts to anchor the
                     catalytic components of the fumarate reductase to the
                     cytoplasmic membrane; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit D"
                     /protein_id="HBI9794223.1"
                     /translation="VFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLAAILT
                     VVTLIGIVTI"
     gene            228..1361
                     /locus_tag="LAF92_005373"
     CDS             228..1361
                     /locus_tag="LAF92_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBI9794224.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTTGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNHRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1450..>1794
                     /gene="blc"
                     /locus_tag="LAF92_005374"
     CDS             1450..>1794
                     /gene="blc"
                     /locus_tag="LAF92_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBI9794225.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGG"
BASE COUNT          452 a          458 c          446 g          438 t
ORIGIN      
        1 gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac
       61 gcgatgcacg atctgaaaat tcacgtgcct gcgggcaaat gggttttcta cggtctggct
      121 gctatcctga cagttgtcac gctgattggt atcgttacaa tctaacgtat cgccaatgta
      181 aatccggccc gcctatggcg ggccgttttg tatggaaacc agaccttatg ttcaaaacga
      241 cgctctgcgc cttattaatt accgcctctt gctccacatt tgctgcccct caacaaatca
      301 acgatattgt gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg
      361 cggtggcggt aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg
      421 ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat
      481 ttacgggcgt gcttggtggc gacgctattg ctcgagggga aatcaagtta agcgatccca
      541 caacaaaata ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc
      601 tcgcaaccta cactactggc ggcctgccat tgcaggtgcc ggatgaggtg aaatcctcaa
      661 gcgacttgct gcgcttctat caaaactggc agcctgcatg ggcgccagga acacaacgtc
      721 tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga
      781 gttttgagca ggcgatgcaa acacgtgtct tccagccact caaactcaac catacgtgga
      841 ttaatgtacc gcccgcagaa gaaaagaatt acgcctgggg atatcgcgaa ggcaaggcag
      901 ttcatgtttc gccaggggcg ttagatgctg aaacttatgg tgtgaagtcg accattgaag
      961 atatggcctg ctgggtacga agcaatatga atcaccgtga tatcaacgac aaaacacttc
     1021 aacaagggat acaactggca caatctcgct actggcaaac cggcgatatg tatcagggtc
     1081 tgggctggga aatgctggac tggccggtaa atcctgacat catcgttaac ggcagtgaca
     1141 ataaaattgc actggcagca cgccccgtaa aagcgattac gcccccaact cctgcagtac
     1201 gcgcatcatg ggtgcataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta
     1261 ttccagaaaa agagctgggt atcgtgatgc tggctaacaa aaactatccc aatccagcga
     1321 gagtcgccgc cgcctggcag attctcaacg ctctacagta aaatttcatc gggtccgaat
     1381 tttcggacct tttctgcgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag
     1441 gaaacattta tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt
     1501 gcctgcagtt ctcctacgcc gccgcgtggc gtaacagtag tgaataattt cgacgccaaa
     1561 cgttatctcg gtacctggta tgagattgcc cgttttgatc accgttttga acgtggactg
     1621 gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg gcctgaatgt cattaataaa
     1681 ggctataacc ctgacagagg aatgtggcag cagagtgaag ggaaagcgta ctttaccggc
     1741 gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg cggt
//
LOCUS       DAEVZA010000428         1703 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.316, whole genome shotgun sequence.
ACCESSION   DAEVZA010000428 DAEVZA010000000
VERSION     DAEVZA010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.316"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            217..675
                     /gene="gpt"
                     /locus_tag="LAF92_005375"
     CDS             217..675
                     /gene="gpt"
                     /locus_tag="LAF92_005375"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBI9794226.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            767..>1703
                     /gene="frsA"
                     /locus_tag="LAF92_005376"
     CDS             767..>1703
                     /gene="frsA"
                     /locus_tag="LAF92_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBI9794227.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQ"
BASE COUNT          389 a          433 c          457 g          424 t
ORIGIN      
        1 acagggttag cagaaaatgt tgtcaacaca agacaggctt gcgagatatg tttgagaata
       61 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
      121 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
      181 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
      241 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
      301 tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt
      361 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
      421 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
      481 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
      541 tttgtcacca tctttgcaaa accggctggt cgtccgctgg ttgatgatta tgttgttgat
      601 atcccgcaag atacctggat cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
      661 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
      721 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
      781 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
      841 ccgctttaat cacggcgcac aaccgcctgt gcagtcggct cttgatggta aaaccatccc
      901 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggtattg acccacgtga
      961 aatcctagac gtccaggcac gtattgtgat gagcgatgct gaacgtaccg acgatgattt
     1021 atacgatact gtgattggct accgtggtgg caactggatt tatgagtggg ccacccaggc
     1081 gatggtgtgg caacaaaaag cctgtgcgga agaagatccg caactcagtg gtcgacactg
     1141 gctgcatgcg gctacgttgt acaacattgc cgcctatcct catctgaaag gagatgacct
     1201 ggccgagcaa gcgcaggctt tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc
     1261 gggcacgatg cggcagatgg agtttaccgt acccggcggt gcgcccatca ccggcttttt
     1321 gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg gtctggatgc
     1381 gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg gcattgcgat
     1441 gctgactatt gatatgccat cggtgggctt ttcttcaaaa tggaagctca cccaggactc
     1501 cagcctgttg catcagcacg tcttaaaggc gctgcctaac gtaccgtggg tggatcacac
     1561 tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct
     1621 tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg gtagttcata ccctgttgag
     1681 tgattttaag tgccagcaac agg
//
LOCUS       DAEVZA010000429         1135 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.340, whole genome shotgun sequence.
ACCESSION   DAEVZA010000429 DAEVZA010000000
VERSION     DAEVZA010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.340"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            29..904
                     /locus_tag="LAF92_005377"
     CDS             29..904
                     /locus_tag="LAF92_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-55"
                     /protein_id="HBI9794228.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(951..>1135)
                     /locus_tag="LAF92_005378"
     CDS             complement(951..>1135)
                     /locus_tag="LAF92_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin fold metalloprotein, WbuC family"
                     /protein_id="HBI9794229.1"
                     /translation="WHAVLSLDTGGIIFEVKHGGYQPVAADDYAHWAPAEGEPGTTEL
                     MAWYAQAQVGDSTFAV"
BASE COUNT          276 a          298 c          321 g          240 t
ORIGIN      
        1 tttcgtctct tccagaataa ggaatcccat ggttaaaaaa tcactgcgcc agttcacgct
       61 gatggcgacg gcaaccgtca cgctgttgtt aggaagtgtg ccgctgtatg cgcaaacggc
      121 ggacgtacag caaaaacttg ccgaattaga gcggcagtcg ggaggcagac tgggtgtggc
      181 attgattaac acagcagata attcgcaaat actttatcgt gctgatgagc gctttgcgat
      241 gtgcagcacc agtaaagtga tggccgtggc cgcggtgctg aagaaaagtg aaagcgaacc
      301 gaatctgtta aatcagcgag ttgagatcaa aaaatctgac cttgttaact ataatccgat
      361 tgcggaaaag cacgtcaatg ggacgatgtc actggctgag cttagcgcgg ccgcgctaca
      421 gtacagcgat aacgtggcga tgaataagct gattgctcac gttggcggcc cggctagcgt
      481 caccgcgttc gcccgacagc tgggagacga aacgttccgt ctcgaccgta ccgagccgac
      541 gttaaacacc gccattccgg gcgatccgcg tgataccact tcacctcggg caatggcgca
      601 aactctgcgg aatctgacgc tgggtaaagc attgggcgac agccaacggg cgcagctggt
      661 gacatggatg aaaggcaata ccaccggtgc agcgagcatt caggctggac tgcctgcttc
      721 ctgggttgtg ggggataaaa ccggcagcgg tggctatggc accaccaacg atatcgcggt
      781 gatctggcca aaagatcgtg cgccgctgat tctggtcact tacttcaccc agcctcaacc
      841 taaggcagaa agccgtcgcg atgtattagc gtcggcggct aaaatcgtca ccgacggttt
      901 gtaatagcgg aaacggaatg gggaaactca ttccgttttt gtttatcgcc ttagacggca
      961 aaagtgctgt cgcccacctg cgcttgcgca taccaggcca taagctccgt ggttcctggt
     1021 tctccttccg ctggagccca gtgcgcatag tcatcggcag ccacgggttg atagccaccg
     1081 tgttttactt caaaaattat gccaccggta tccagcgaca gcacggcatg ccagg
//
LOCUS       DAEVZA010000430         1114 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.344, whole genome shotgun sequence.
ACCESSION   DAEVZA010000430 DAEVZA010000000
VERSION     DAEVZA010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.344"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            36..779
                     /locus_tag="LAF92_005379"
                     /pseudo
     CDS             36..779
                     /locus_tag="LAF92_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TEM family class A beta-lactamase"
     gene            1038..>1114
                     /locus_tag="LAF92_005380"
     CDS             1038..>1114
                     /locus_tag="LAF92_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin fold metalloprotein, WbuC family"
                     /protein_id="HBI9794230.1"
                     /translation="MKQITMSDMQQQSAAAVQSPRLRAHR"
BASE COUNT          310 a          273 c          269 g          262 t
ORIGIN      
        1 acaaaaggct gcctcatcgc taactttgca acagtgcccg agtgggttac atcgaactgg
       61 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
      121 gcacttttaa agttctgcta tgtggtgcgg tattatcccg tgttgacgcc gggcaagagc
      181 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
      241 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
      301 gtgataacac tgctgccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
      361 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
      421 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
      481 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
      541 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
      601 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
      661 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
      721 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
      781 tgtcagacca agtttactca tactgaaata attggggggt gattaccacc gtaaattttt
      841 cccagacggt aaaaagaatt acagactttt atcaactaaa aaagtggcct gaatatcagc
      901 actaccgtca ataccatttc agtaaatcag gatagggaaa aattggcaaa cccagttctt
      961 cacttgctct gcgggttctg tcaccctgac gtcatacggt aatccccccc aattcattaa
     1021 tcgacaaggt catcctgatg aaacaaatta ccatgagcga tatgcagcaa cagagcgcag
     1081 cggctgtgca atcgcctcgg ctccgcgcgc accg
//
LOCUS       DAEVZA010000431          988 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.350, whole genome shotgun sequence.
ACCESSION   DAEVZA010000431 DAEVZA010000000
VERSION     DAEVZA010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.350"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            65..853
                     /locus_tag="LAF92_005381"
     CDS             65..853
                     /locus_tag="LAF92_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HBI9794231.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
BASE COUNT          206 a          286 c          283 g          213 t
ORIGIN      
        1 ctgcgcaaaa gtattccaca aagccgcaac ttaaaagctg ccgctgaact taacgttagg
       61 catcatgggt gaatttttcc ctgcacaagt tttcaagcag ctgtcccacg ctcgcgcggt
      121 gatcgagcgc catctggctg cgacactgga cacaatccac ctgttcggat ctgcgatcga
      181 tggagggctg aagccggaca gcgacataga cttgctcgtg accgtcagcg ccgcacctaa
      241 cgattcgctc cggcaggcgc taatgctcga tttgctgaaa gtctcatcac cgccaggcga
      301 tggcggaaca tggcgaccgc tggagctaac tgttgtcgct cgaagcgaag tagtgccttg
      361 gcgctatccg gcgcggcgtg agcttcagtt cggtgagtgg ctccgccacg acatcctttc
      421 cggaacgttc gagcctgccg ttctggatca cgatcttgcg attttgctga ccaaggcgag
      481 gcaacacagc cttgcgcttc taggcccatc cgcagccacg tttttcgagc cggtgccgaa
      541 ggagcatttc tccaaggcgc ttttcgacac tattgcccag tggaatgcag agtcggattg
      601 gaagggtgac gagcggaacg tcgttcttgc tcttgctcgc atttggtaca gcgcttcaac
      661 tggtctcatt gctcctaagg acgttgctgc cgcatgggta tcggagcgtt tgcctgccga
      721 gcatcggccc ctcatctgca aggcacgcgc ggcgtacctg ggtagcgagg acgacgacct
      781 agcaatgcgc gtcgaagaga cggccgcgtt cgttcgatat gccaaagcaa cgattgagag
      841 aatcttgcgt tgagcggcat gtgcgaaaag tgcatcgacc cgcgccgagg gcatctgatg
      901 cctaactcgg cgttcaagcg gacgggctgc gcccgccgct caactatgcg ttagatgcac
      961 taagcacata attgctcaca gccaaact
//
LOCUS       DAEVZA010000432          988 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.351, whole genome shotgun sequence.
ACCESSION   DAEVZA010000432 DAEVZA010000000
VERSION     DAEVZA010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.351"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            83..958
                     /locus_tag="LAF92_005382"
     CDS             83..958
                     /locus_tag="LAF92_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-65"
                     /protein_id="HBI9794232.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAVAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAERR
                     RDVLASAARIIAEGL"
BASE COUNT          226 a          257 c          313 g          192 t
ORIGIN      
        1 tgatttttga agctaataaa aaacacacgt ggaatttagg gaatactgat gtaacacgga
       61 ttgaccgtat tgggagtttg agatggtgac aaagagagtg caacggatga tgttcgcggc
      121 ggcggcgtgc attccgctgc tgctgggcag cgcgccgctt tatgcgcaga cgagtgcggt
      181 gcagcaaaag ctggcggcgc tggagaaaag cagcggaggg cggctgggcg tcgcgctcat
      241 cgataccgca gataatacgc aggtgcttta tcgcggtgat gaacgctttc caatgtgcag
      301 taccagtaaa gttatggcgg tcgcggcggt gcttaagcag agtgaaacgc aaaagcagct
      361 gcttaatcag cctgtcgaga tcaagcctgc cgatctggtt aactacaatc cgattgccga
      421 aaaacacgtc aacggcacaa tgacgctggc agaactgagc gcggccgcgt tgcagtacag
      481 cgacaatacc gccatgaaca aattgattgc ccagctcggt ggcccgggag gcgtgacggc
      541 ttttgcccgc gcgatcggcg atgagacgtt tcgtctggat cgcactgaac ctacgctgaa
      601 taccgccatt cccggcgacc cgagagacac caccacgccg cgggcgatgg cgcagacgtt
      661 gcgtcagctt acgctgggtc atgcgctggg cgaaacccag cgggcgcagt tggtgacgtg
      721 gctcaaaggc aatacgaccg gcgcagccag cattcgggcc ggcttaccga cgtcgtggac
      781 tgtgggtgat aagaccggca gcggcgacta cggcaccacc aatgatattg cggtgatctg
      841 gccgcagggt cgtgcgccgc tggttctggt gacctatttt acccagccgc aacagaacgc
      901 agagcgccgc cgcgatgtgc tggcttcagc ggcgagaatc atcgccgaag ggctgtaact
      961 ggttttgttg aataaatcga acttttgc
//
LOCUS       DAEVZA010000433          929 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.355, whole genome shotgun sequence.
ACCESSION   DAEVZA010000433 DAEVZA010000000
VERSION     DAEVZA010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.355"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            46..837
                     /locus_tag="LAF92_005383"
     CDS             46..837
                     /locus_tag="LAF92_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HBI9794233.1"
                     /translation="MRVAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQ
                     DLFEALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
BASE COUNT          243 a          227 c          248 g          211 t
ORIGIN      
        1 gatgttacgc agcagggcag tcgccctaaa acaaagttag acatcatgag ggtagcggtg
       61 accatcgaaa tttcgaacca actatcagag gtgctaagcg tcattgagcg ccatctggaa
      121 tcaacgttgc tggccgtgca tttgtacggc tccgcagtgg atggcggcct gaagccatac
      181 agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
      241 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
      301 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
      361 gaactgcaat ttggagaatg gcagcgcaat gacattcttg cgggtatctt cgagccagcc
      421 atgatcgaca ttgatctagc tatcctgctt acaaaagcaa gagaacatag cgttgccttg
      481 gtaggtccgg cagcggagga attctttgac ccggttcctg aacaggatct attcgaggcg
      541 ctgagggaaa ccttgaagct atggaactcg cagcccgact gggccggcga tgagcgaaat
      601 gtagtgctta cgttgtcccg catttggtac agcgcaataa ccggcaaaat cgcgccgaag
      661 gatgtcgctg ccgactgggc aataaaacgc ctacctgccc agtatcagcc cgtcttactt
      721 gaagctaagc aagcttatct gggacaaaaa gaagatcact tggcctcacg cgcagatcac
      781 ttggaagaat ttattcgctt tgtgaaaggc gagatcatca agtcagttgg taaatgatgt
      841 ctaacaattc gttcaagccg accgcgctac gcgcggcggc ttaactccgg cgttgtgctg
      901 acgaaaaaaa acaaaaatct gcgtatacc
//
LOCUS       DAEVZA010000434          929 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.356, whole genome shotgun sequence.
ACCESSION   DAEVZA010000434 DAEVZA010000000
VERSION     DAEVZA010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.356"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            80..901
                     /gene="lnu(F)"
                     /locus_tag="LAF92_005384"
     CDS             80..901
                     /gene="lnu(F)"
                     /locus_tag="LAF92_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(F)"
                     /protein_id="HBI9794234.1"
                     /translation="MLQLKMIELFKEGCHEDARIIAALMFGSFAIGEGDEFSDIEFAV
                     FIQDDHFENFDQRSWLNAVSPVAAYFPDDFGHHTALFENGIRGEFHFMRKSDIPVIST
                     WQGYGWFPSLEAAVLLDRSGELSRYASALVGGPPIREGAPLVEGLVLNLISLMLFGAN
                     LLNRGEYARAWALLSKAHENLLKLVRLHEGATDHWPTPSRALEKDISEDSYNRYLACT
                     SSAEPRALCAAYHQTWTWSLELFKSVTEPLNIELPRTVIAQAKRLLNESATPHNK"
BASE COUNT          237 a          228 c          240 g          224 t
ORIGIN      
        1 gcgttgtgct gacgaaaaaa aacaaaaatc tgcgtatacc tttcctcata atctgtggct
       61 tcaataaaag gatatttcta tgcttcaact gaaaatgatc gaactcttca aggaaggttg
      121 tcatgaggat gcacgaataa tcgcggcatt gatgttcggc tcatttgcta tcggagaggg
      181 tgacgagttc tctgatatcg aattcgcagt gttcatccag gatgaccatt ttgaaaattt
      241 cgatcagcgc tcgtggctta atgccgtaag tccggttgct gcttactttc cggacgactt
      301 cggccaccac accgcacttt ttgaaaacgg cattcgcggt gaattccatt tcatgcgaaa
      361 atcggacata ccggtcattt ccacttggca aggctatggg tggtttccct cgcttgaggc
      421 ggctgttttg ttggaccgat caggagagtt gtcaaggtac gcaagcgctc tcgtgggcgg
      481 tcccccgata cgtgaaggcg cgccgctggt ggaagggctt gtgttgaacc tcatcagcct
      541 gatgctcttt ggggccaatc ttttaaatcg gggagagtac gctcgcgcct gggctttgct
      601 cagcaaagca catgaaaacc tactcaagct ggttcgactc cacgaagggg caacagacca
      661 ctggccgaca ccttcacgcg cgctcgaaaa ggatatctcg gaggactcgt ataatcgcta
      721 tctggcatgc acaagcagtg cagaaccaag agcactatgt gcagcctatc atcaaacgtg
      781 gacgtggagt ctcgaattgt tcaagagcgt gacagaacct ctgaatatcg agcttccgag
      841 aactgtaatt gcgcaggcaa aaaggttgct caatgagtct gcgacgccgc acaacaagta
      901 aatccagcgg acgcataaaa acgcgccgc
//
LOCUS       DAEVZA010000435          810 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.365, whole genome shotgun sequence.
ACCESSION   DAEVZA010000435 DAEVZA010000000
VERSION     DAEVZA010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.365"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            362..778
                     /locus_tag="LAF92_005385"
     CDS             362..778
                     /locus_tag="LAF92_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosfomycin resistance glutathione transferase
                     FosA3"
                     /protein_id="HBI9794235.1"
                     /translation="MLQGLNHLTLAVSDLASSLAFYQQLPGMRLHASWDSGAYLSCGA
                     LWLCLSLDEQRRKTPPQESDYTHYAFSVAEEEFAGVVALLAQAGAEVWKDNRSEGASY
                     YFLDPDGHKLELHVGNLAQRLAACRERPYKGMVFFD"
BASE COUNT          175 a          170 c          253 g          212 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgccg taattattta ggatatagct
       61 ctgtgattta gtctttttag ggcgtttatt tatgtcttta tgctgtaatg tcggtgctga
      121 tttggtcttc aataaggcga tttttagccc gctttttccg gggggatgaa atgttattgg
      181 attgctaaat gatttcaaaa aaggggtaac atagagcggg gattagtgtg gcgaggcgca
      241 ggtttccgat ggcaggctaa acgcaaaaaa tgcgcttttt agccggtgat gaggtgaggc
      301 cgggagggga tgcgtcgccg atcacagttt acaacagggt ttgataacgg gaggaaaagt
      361 catgctgcag ggattgaatc atctgacgct ggcggtcagc gatctggcgt caagcctggc
      421 attttatcag cagttacctg gaatgcgcct gcacgccagc tgggatagcg gagcctatct
      481 ctcctgtggg gcgctgtggc tgtgcttgtc gctggatgag cagcggcgta aaacgccccc
      541 tcaggaaagc gactataccc actacgcctt cagcgtggcg gaagaagagt ttgccggggt
      601 ggtggctctg ctggcgcagg cgggggctga ggtatggaaa gataaccgca gtgaaggggc
      661 gtcttactat tttctcgacc ctgacggcca taagctggag ctgcatgtgg ggaatctggc
      721 gcagcggctg gccgcctgtc gcgaacgccc ctacaagggg atggtctttt ttgattgacg
      781 ggttagttca gcttactgcc ggatttcaac
//
LOCUS       DAEVZA010000436          530 bp    DNA     linear   BCT 14-SEP-2021
DEFINITION  TPA_asm: Escherichia coli isolate water
            SAMN20875998-rid12978473.guided.393, whole genome shotgun sequence.
ACCESSION   DAEVZA010000436 DAEVZA010000000
VERSION     DAEVZA010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20875998
            Sequence Read Archive: SRR15565866
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001129047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001129047.1
            Genome Coverage       :: 27x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/13/2021 22:05:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,300
            Genes (coding)                    :: 5,163
            CDSs (with protein)               :: 5,163
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 70 of 137
            Pseudo Genes (internal stop)      :: 32 of 137
            Pseudo Genes (multiple problems)  :: 26 of 137
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20875998-rid12978473.guided.393"
                     /isolate="water"
                     /isolation_source="irrigation water"
                     /db_xref="taxon:562"
                     /country="Ecuador"
                     /lat_lon="0.208677 S 78.493526 W"
                     /collection_date="2019"
                     /collected_by="Lorena Montero, Jorge Irazabal, Jay Grahan,
                     Gabriel Trueba"
     gene            9..482
                     /locus_tag="LAF92_005386"
     CDS             9..482
                     /locus_tag="LAF92_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HBI9794236.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
BASE COUNT          170 a           91 c          103 g          166 t
ORIGIN      
        1 gagttaaatt gaaaatatca ttgatttctg cagtgtcaga aaatggcgta atcggtagtg
       61 gtcctgatat cccgtggtca gtaaaaggtg agcaactact ctttaaagcg ctcacatata
      121 atcaatggct ccttgtcgga agaaaaacat ttgactctat gggtgttctt ccaaatcgca
      181 aatatgcagt agtgtcaaag aacggaattt caagctcaaa tgaaaacgtc ctagtttttc
      241 cttcaataga aaatgctttg aaagagctat caaaagttac agatcatgta tatgtctctg
      301 gcgggggtca aatctataat agccttattg aaaaagcaga tataattcat ttgtctactg
      361 ttcacgttga agtcgaaggt gatatcaaat tccctataat gcctgagaat ttcaatttgg
      421 tttttgaaca gttttttatg tctaatataa attatacata ccagatttgg aaaaaaggct
      481 aacaatgcgt tgcagcacca gtcgcttcgc tccttggaca gcttttaagt
//
